LOCUS       DASRBV010000001         9329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_132,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000001 DASRBV010000000
VERSION     DASRBV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFA02_00005"
     CDS             complement(<1..764)
                     /locus_tag="VFA02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU08255.1"
                     /translation="MQQLRYIPPPPLGQFVQCIWYCESDTAPSHTHERLMPTGASTIV
                     FNLRDDDIRIYRPNDITQYDSFGTAVFSGARSNFFVIDTVAQQRVVGIQFHPGGSFPF
                     FHIPADHAKNAEITLEDLWRARAGELRERMLAAPSAAAIFSILERDLLAQLARPLELH
                     PAVEYAVYHMRRVPHITTVRSITEKIGLSQRRFIQLFSQQVGLAPKTFCRVRRFQRVL
                     QKVYGMSEVEWTQLALDCGYYDQAHFIHDFQAFSGIT"
     gene            complement(835..2136)
                     /gene="aroA"
                     /locus_tag="VFA02_00010"
     CDS             complement(835..2136)
                     /gene="aroA"
                     /locus_tag="VFA02_00010"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZU08256.1"
                     /translation="MLDTKTERIIAPARNILGSLRLPGDKSISHRYALLSALASGTTK
                     LTNFSTGADCASSLGCAAALGAKISRLENGVIEITGTGGQLQESSAPLDCGNSGSTMR
                     MLTGLLAATPHTYTLVGDESLSRRPMERVRKPISQMGAEITLTDGHAPLTVRGGKLKG
                     IEYTTPVPSAQVKTALLFAGFQANGTTTVRESVRTRDHGELALRAFGAEVERTTDSIS
                     IAGGQQLHGIEATVPGDISSAAFFLCAAALFPGSNLIFDALGLNPTRAALLDVLTALG
                     AHIGVLNLEEKHGELVGTVQVNAPAHGLTGTTISGGLSAQLIDELPVLAAIAPYTQNG
                     IRIRDAKELRVKESDRIALVANNLRAMGAEVEEFEDGLDVPGGQQLHGAEINSGGDHR
                     IAMAFSVAALRASGETVVRGAESASISFPEFYDLLDRVAER"
     gene            2219..3379
                     /locus_tag="VFA02_00015"
     CDS             2219..3379
                     /locus_tag="VFA02_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU08257.1"
                     /translation="MKVLLVHNRYQQKGGEDSVVESEGKLLAKNGTEVELLETNNDGI
                     VGIRGKLDAAASVFYSFRGVDLLKEAIRRKRPDVVHVHNWFPTFSPGIFWACKKMKVP
                     VVHTLHNYRLLCAMASLYRDGQVCEDCIGTAFRTPGVLHGCYRGSRSGTAAATAGMLI
                     HWKLGTWRKVVDRFIALSEFAKSKLVQGGLPAEKIAVKANCLDYDPGMRPGDGGYFLY
                     VGRLTAEKGIPVLLECWKHGPGLPPLHIAGVGPLENEVRAASSTLTNVKWLGAKSSDE
                     VIEIMGRAKALVCPSLWYEGMPRVVIEAMAVGTPVIASSIGTYPEMIIPYKTGMLFEV
                     NEPSALLACLREIDDRSDYQGWRKQARNKFLTCYSPEVNLKALRTIYAQVMG"
     gene            complement(3382..4185)
                     /locus_tag="VFA02_00020"
     CDS             complement(3382..4185)
                     /locus_tag="VFA02_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZU08258.1"
                     /translation="MNTLAHDRVNILGVGISVLDVPSAVRSVDSFLASGRKGYICVTG
                     MHGIMEAQADPVFRQIQNNSIFTTPDGIPTVWIGHLMGHKNMKQVAGPQFMMEVCAAA
                     AKNGSRHFLYGGKPGVAERLKQILTERFPGLQIVGTYTPPFRPLNAEEEDELYRLVRD
                     SGADVMWCGISTPKQERFMAKYIDVLPVKLMIGVGAAFDINSGLLKDSPEWAKKCGLQ
                     WFHRLCQEPRRLWRRYLLNIPRFVGLYMMQWSGLRSYKLDIPSRPHAIL"
     gene            4697..6991
                     /locus_tag="VFA02_00025"
     CDS             4697..6991
                     /locus_tag="VFA02_00025"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU08259.1"
                     /translation="MGEDVSSGYIAGPAGEQSLKDIWRVLRKRKWWILSFCLGGTSLA
                     VLACILLTNQYKSTATVQVGKDETVEVSLNRNNGGPTLSESDTKTDIATHIAVLESDN
                     IALAVIQDLHLENYKPFAFKPTWLGWITGSNARIQDELNRHLPLSESPARRERLLTLF
                     SKKLQVKNPPDTRLITVSFLNPNPQLAASIANTVVDEYVKFESQSRSSTEASEWLESQ
                     LQQLKEKMDNSQKELADYERETGLNSLLLKSIGEAGGAGAVTHIPVLDKLDTLNQELT
                     QAEANRVAKEAIYHLTQTRDSNIVANLASSSLPQIATSAAITQGNGLELLQSLRQQKA
                     QLELAYSDANTRYGARNPRILELQNQLASVNKQIAEELEQINLRAKNDYLLALKNEEG
                     LRAAFANQQAAASKLNESTVRLQMLAQQATSSRQLYDALYGQLQEANVQAGLRATNIR
                     IADAARPPASPARPNPPLYVAIGLAAGLLFGVSSAFVREHMDETVRTHLHLLPLTRLP
                     VLASIPNVPDALLASSSKRGLLQRKTEQASPLLTRPRSAMAEAYRTLRTSIALASAGR
                     PLHTLLVTSPLAGEGKTSVSYNTAMAFACAGKKVLLVDADLRHPQLHEYFHCSQSPGL
                     SDVLAEDKPFASLIQPHPLVRNLYLLPAGEPSPTSGELLASAKLDDLLNMLKQSYGLV
                     ILDSPPLLLTADAVALSAKVDATLGVVRAEVTNKTAVERAAAVLERASRHSVGMVLNG
                     VNTHSIEYYQAYGHNGGGKYYEEN"
     gene            6993..8021
                     /locus_tag="VFA02_00030"
     CDS             6993..8021
                     /locus_tag="VFA02_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU08260.1"
                     /translation="MDNIARLLTNSMLCAALTGLATYAVPASAQQAAPIAPVSSSSLP
                     QSSSQPARQSGSTTNGALTAVPDDFSDLRLAPGFLLNISVYDEPEFDTHVRVDEKGDI
                     SLPLLGSIQVAGKTIPEAQKAIQDKFRAAQILKDPQVTLNVDQYVSSSVTVLGEVQSP
                     GRIQLLAPHNLLDVIGMAGGETNLAGDTIQVENPSEGGNAVHIYHYARGRDENEVRNV
                     MVKPGDIIRVKRAGIVYVLGAVNRPGGYVMQEDGALDVAQAISMAMGTTMQAKIGGIR
                     VVRHDQNGNITEIPVAYKDIVGGKEKPLQLEAQDIVYVPVSKLKSVFTAGSTIVGQTG
                     AATIYAVK"
     gene            8034..>9329
                     /locus_tag="VFA02_00035"
     CDS             8034..>9329
                     /locus_tag="VFA02_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZU08261.1"
                     /translation="MLNYVNTESEQVSIYPWDIESWRSAMRIAGIPGLALLSLLLGTM
                     IQYADPSSGGPAHTSTTMLVLVRGPLCVLAFLLLLFQPHAPRLRLKDTRVLFFAFAIL
                     YLVSTLWSTEHKGTLGKSVEIFLAGVVFWEVSRTRNALQRVEALRQIILLTISLVAFV
                     TVTGYFLHIHAFIQPRPGIFTRTTAQAPFLSGNGLGYVSSALFLVVFAEWQAKRLDCG
                     PALRQMTFAMFMFAFAASRTSFAILVLGVIFVVARKSSSLGSFLAAATFALGLTFQDN
                     IMVALQGKEATSDFTTLSGRTVMWTAAIRQWQKHPLLGAGGGVGGKETLKHIGNFSLQ
                     TASSLHNGFMELLTGLGAVGFALGVFLLVLVTLRTWKAWRENPQYAGTYVLIIHVWMT
                     TVMSTGVLGWMGYEMALFLCIMTNLDLVRHRPAYAYQRVV"
BASE COUNT         2243 a         2322 c         2585 g         2179 t
ORIGIN      
        1 gtaatcccgg aaaaggcctg aaaatcgtgg atgaagtgcg cctgatcgta gtagccgcag
       61 tcgagcgcca actgcgtcca ttcgacctcg ctcattccgt aaaccttctg cagaacgcgc
      121 tggaagcggc gcaccctgca aaatgtttta ggggccagtc ccacctgctg gctgaaaagc
      181 tgaataaagc gccgctggct cagcccaatc ttctcagtga tagagcgcac cgttgtgatg
      241 tgcgggacgc ggcgcatgtg gtacacagcg tattccacag caggatgcaa ttcgagaggc
      301 cgcgcaagct gggccagcag gtcccgctcc aggatggaaa agatggcagc cgcagatggc
      361 gctgcaagca ttcgttcccg aagttctccg gcgcgcgccc gccacaggtc ttcgagagta
      421 atctctgcat tcttcgcatg gtcagcaggt atatggaaga aaggaaacga tccgccggga
      481 tgaaactgga ttcccacgac ccgctgctgg gcgactgtgt caatgacaaa gaagttggag
      541 cgtgctccag agaagacggc agtgccaaag gaatcgtatt gtgtgatgtc attcgggcga
      601 tagatgcgga tgtcgtcatc acgcaggttg aagacaatgg tagatgcgcc tgtgggcatc
      661 agccgctcat gcgtatgcga tggggccgta tccgattcgc aataccagat gcactggacg
      721 aactgtccaa gcggaggagg cggaatataa cgcagctgct gcataagtcc aacacggcga
      781 gtgtacgccg ctttctaatt cgagaaaaga aggacagacc caggccccgg tttcttaccg
      841 ttcggcgacc cggtcgagca gatcataaaa ttcggggaag gaaatggacg ctgattccgc
      901 gccccgaacc actgtttcgc ctgaagcccg cagggccgcc actgagaaag ccatggcgat
      961 acgatggtcg ccgccggagt tgatctctgc cccgtgaagt tgctggcctc cagggacatc
     1021 caggccgtcc tcaaattcct cgacttcagc gcccatagcg cgcaggttgt tggcgacgag
     1081 cgcaatacgg tcggactctt tgactcgaag ctctttagcg tcgcggatcc ggatgccatt
     1141 ctgcgtgtat ggcgcaatcg cggcgagcac tggcagttca tcaatcaatt gagccgagag
     1201 accgcctgaa atggtggtgc ccgtcagccc gtgggcggga gcatttacct gtactgtccc
     1261 caccagctcg ccatgttttt cctcaagatt gagcacgcca atgtgggcgc caagtgcggt
     1321 gagcacgtca agcaaagcgg cgcgggtagg gttcagtcca agcgcgtcga agatgaggtt
     1381 tgagcctgga aagagcgcag cggcgcaaag aaaaaatgct gccgaggaga tgtctccggg
     1441 aacggtggcc tcaatgccat gcaattgctg gccgccggcg atgctgatgg agtcagtggt
     1501 gcgttcgact tctgcgccga aggcacgcaa cgccagctct ccgtggtcac gtgtccggac
     1561 ggactcccgt acagtggtgg tgccattcgc ctgaaatccg gcaaaaagca gcgctgtttt
     1621 tacctgcgca ctgggcacgg gcgtagtgta ttcaatgcct ttgagcttgc ctccacggac
     1681 agtaagcgga gcatgaccat cggtgagtgt aatctcagcg cccatctgcg agatgggttt
     1741 acgcacgcgc tccatgggac ggcggcttaa ggactcgtcg ccaaccagcg tataggtgtg
     1801 cggagtggcc gccagcagcc cggtaagcat acgcatggtt gagccggaat tgccgcagtc
     1861 aagaggggcg ctggattcct gcagctggcc gcccgtcccg gtgatttcga ttacaccatt
     1921 ttcgagacgc gaaatctttg cacccagggc cgcagcgcag ccgagactgc tggcgcagtc
     1981 cgccccggtg gaaaaattgg tgagcttcgt ggtcccggaa gcaagcgcgc tcaaaagagc
     2041 gtagcgatgt gaaatactct tgtctccagg caaacgaagg cttcccagga tgttccgcgc
     2101 aggtgcaatg atgcgttcag ttttcgtgtc gagcattact tttatcgtag caggatgcag
     2161 attctttgcg cggcggttgt agaaatggga ttctaacggt atatatccaa atatctgcat
     2221 gaaagtactg ctcgttcaca accgttacca acaaaagggt ggtgaagact ctgttgtcga
     2281 atcagaaggg aaacttctgg caaaaaacgg tacagaggtc gagctgcttg agacaaacaa
     2341 tgacggaatc gttggtatca ggggcaaact cgatgcagct gcctcggtct tttatagctt
     2401 tcgcggcgtg gacctgctga aggaagccat cagacggaaa aggccggatg tagtccacgt
     2461 gcataactgg tttcccacgt tctctcccgg aatcttctgg gcgtgtaaaa agatgaaagt
     2521 tccagtcgtg catacattgc ataactaccg cttgctgtgt gccatggcat ctttgtatcg
     2581 agacgggcag gtctgtgagg attgtattgg cactgctttc cgaactcccg gcgtccttca
     2641 tggatgctat cgaggcagcc gttccggtac agcggctgcc actgccggaa tgcttattca
     2701 ctggaaactt gggacatggc gcaaagtggt ggacagattc attgcgctat cagagtttgc
     2761 caaaagcaag ttagtgcagg gaggtcttcc ggcggaaaaa atcgcggtga aggcaaattg
     2821 cctcgattat gatccgggga tgcgtccggg cgatggcggc tactttcttt atgttggccg
     2881 gctgacagca gagaagggga tcccagtcct tttggaatgc tggaaacatg ggcccggtct
     2941 tccgccgctg cacattgcgg gtgtgggccc tcttgaaaac gaagtgcggg cggcctccag
     3001 tacgctaaca aatgtaaagt ggctgggcgc aaagagcagc gatgaagtga tcgagattat
     3061 gggacgggca aaagcattgg tttgtccttc tttgtggtat gaaggaatgc cgagggtcgt
     3121 gattgaagca atggcggtgg gtactcctgt gatagcttcg agtatcggaa cttatcctga
     3181 gatgataatt ccatataaga ctggaatgct gtttgaagta aatgaaccat ctgcactgct
     3241 ggcttgtttg cgggaaatag atgaccgttc tgactatcag ggatggagaa aacaggcaag
     3301 gaacaagttt cttacctgtt acagtccaga ggtaaatctt aaggccctgc gcacaattta
     3361 tgcacaggta atgggataag tttataagat tgcatgtgga cgcgacggta tatctaactt
     3421 ataagaccgt agtccagacc actgcatcat ataaagacct acaaagcgag gaatattgag
     3481 gagatagcgg cgccaaagcc tccgcggctc ttgacacaag cggtggaacc attgcaggcc
     3541 gcatttcttg gcccattcag gcgagtcctt cagaagtccg ctgttaatgt cgaaagcagc
     3601 gcctacgccg atcataagtt tgacaggcag gacatcaata tacttggcca tgaagcgttc
     3661 ctgcttcgga gtgctgattc cacaccacat aacgtccgct cctgaatcgc gcaccagccg
     3721 gtaaagctca tcttcttcct ccgcattcag cgggcgaaac ggtggggtat acgtgccgac
     3781 aatttgcagg ccaggaaagc gctctgtaag gatttgcttc aggcgttcgg ccactcctgg
     3841 cttgccgcca tatagaaagt gtctggagcc attttttgcg gcggcagcgc agacttccat
     3901 cataaattgc ggcccagcta cctgtttcat gtttttatgg cccattaagt ggccaatcca
     3961 aactgtaggt attccatccg gagttgtaaa aatcgagtta ttttggatct ggcggaatac
     4021 aggatctgcc tgggcctcca tgattccatg catgccagtt acacagatat agccttttct
     4081 tcccgatgcc agaaatgaat ctacagaacg gaccgccgag ggaacatcca gtacgcttat
     4141 gccaactccg agaatattta ctctgtcatg tgcaagtgta ttcattattt ttttgtcctt
     4201 ttagccagaa aaaaggcagc acggtccaga aaaaagaaaa ccggcagaaa tttacaaact
     4261 atgtggcaat tagttccagc ccgtaaatat tgctaaatgt ggcccattct gattcagcgt
     4321 tttggggccg tgttctttat gaaagtgacg ttaacatcct attaatatgc gcttcaatag
     4381 acggaatgta ctgatacaag cggcgcaagg ctatgagata tttttcctac ttatcggtat
     4441 ccgtatatct cattgacatg tgtgatgtac gacttagact gaaaaagcgt cttgattttt
     4501 gtatttagcg gcacaaaatt ttactgtttt attcaatact tatacgcatt agtacttgca
     4561 ttaaagcctt gtatcagcgc aatccttcag cgtgaaacga tgcgagttag atatttcgac
     4621 tttcagggag taactatcca tgcttgttcc tgtaataact gaacctggaa accaaggcag
     4681 gcttcgcgcc tcttacatgg gcgaggatgt ttcatccgga tatatagcgg gtccggccgg
     4741 cgagcaatcc ttgaaagaca tctggcgcgt tttgcgcaag cgcaagtggt ggatactttc
     4801 attttgcctg ggtggaacaa gcctagcggt gcttgcatgc attttgctga cgaaccagta
     4861 caagagcacg gcaacggtcc aggtgggcaa ggatgaaacc gtcgaggtga gcctgaaccg
     4921 caataacggc ggtcctacct tgagcgaaag cgataccaaa acggacattg cgacgcacat
     4981 tgccgtgctg gagagcgata acattgccct ggcagttatc caggacctgc atcttgagaa
     5041 ttacaagcca tttgcgttta agccgacctg gcttggctgg ataacaggaa gtaatgcgcg
     5101 aatacaggac gagcttaacc gtcatcttcc gttgagtgag tcaccagcca ggcgtgagcg
     5161 cctccttact cttttttcca agaagcttca ggtaaagaat ccgccagata cgcggctgat
     5221 tactgtaagt tttctaaatc ccaacccgca attggctgca agtattgcga atactgttgt
     5281 agatgaatac gtgaagtttg agtcccaatc gcgttcttca acggaagcat cggagtggct
     5341 cgaatcccag ctccagcaat tgaaggaaaa gatggataac tcgcaaaagg agctcgcaga
     5401 ttatgagcgc gaaaccggac tgaacagcct tttgctgaaa tccattggtg aagctggagg
     5461 agcgggcgcg gtaacgcaca ttcccgtact cgacaagctg gatactctca accaggaact
     5521 gactcaggca gaagcgaacc gggtagcaaa ggaagcaatt taccatctta cgcagaccag
     5581 agactcaaac atcgttgcaa atctggcatc gagctcactg ccgcagattg caacttcggc
     5641 tgcaattacg caaggcaatg ggcttgagct tctgcaaagt ctgcgccaac agaaggcgca
     5701 gttagagctg gcctattctg acgcaaatac cagatacgga gccagaaacc ctcgaattct
     5761 ggaactgcaa aaccagcttg cgagtgtaaa caagcagatt gctgaagaac tggagcagat
     5821 caatcttcgc gcaaagaacg actatttgct tgcgctgaag aacgaagaag gccttcgtgc
     5881 cgctttcgcc aatcaacagg ccgctgcgag caagctgaac gagagcacag tcaggctgca
     5941 gatgctggcc cagcaggcaa catcgagccg tcaattgtat gacgcattgt atggacaatt
     6001 gcaggaggcc aacgttcagg ccgggcttcg cgccacaaat atcaggattg ccgatgctgc
     6061 acggccgccg gccagtccgg cgcggcctaa tcctcctttg tatgtggcga tagggttagc
     6121 ggctggttta ttgtttggcg tgtcttctgc atttgtccgc gagcacatgg atgagacggt
     6181 gagaacacat ctccatctgc ttccacttac acggctccca gttctggcaa gcattcccaa
     6241 tgttcctgat gcactgctgg catccagcag taagcgagga ctgttgcaga gaaagacaga
     6301 acaagccagt cctttgctga ctcgtccgag gtccgccatg gcagaagcgt acaggacttt
     6361 acgcacctct attgcgctgg catctgcggg acgtcctctc cacacgcttt tggtgacgag
     6421 tccattggcc ggcgaaggga agacatctgt gtcctacaac acggcaatgg catttgcgtg
     6481 tgccggaaag aaggtgctct tagtggatgc cgacctgcgt catcctcagc ttcatgagta
     6541 ttttcattgc agtcaatctc caggcctgag cgatgtactg gcagaggaca agccgtttgc
     6601 ttcgctgatc caaccgcatc cgctggtccg caatttatat ctgctacctg caggcgagcc
     6661 gtcgcccaca tcgggtgaac tgcttgcttc tgctaaattg gatgaccttc tgaacatgct
     6721 gaaacagtct tacggactgg tgattttaga tagtccgccg ctgcttctga cggcagatgc
     6781 cgttgctctg tctgcaaaag tagatgccac gctcggagta gtacgggcag aagtgacaaa
     6841 caagactgcg gtagagcgcg cagcagctgt tcttgaacgg gccagcaggc attcagttgg
     6901 aatggtgctg aatggtgtga atactcattc catagagtat taccaggcct acggacacaa
     6961 cggcggtgga aagtactatg aggaaaatta gtatggacaa cattgcgcgc ttacttacca
     7021 actcgatgtt atgtgcggcg ctgactggcc tggcgacgta tgcagtgccg gcatcggcac
     7081 agcaggcagc gcctatagct cccgtaagca gctcctcgct tccacagtcg agcagccagc
     7141 cagcgcgcca gtctggcagt acgacaaatg gtgctcttac ggctgtgccg gatgactttt
     7201 ctgacttgcg gctcgcgcct ggatttttgc tgaacatttc tgtttacgac gagccggaat
     7261 tcgatactca cgtgcgcgta gatgaaaaag gagacatcag ccttccgctt cttggaagta
     7321 tccaggtggc cggaaagaca atcccggaag cgcagaaagc gattcaggac aagttccgcg
     7381 ctgcacagat actgaaagat ccgcaggtga cgctgaatgt agaccagtat gtatcatcca
     7441 gcgtgactgt gttaggagaa gtccagtcgc caggacgtat tcagttactc gctccgcata
     7501 acttgctgga cgtcattgga atggccggag gcgagacgaa tcttgcgggg gacacgatcc
     7561 aggtagaaaa cccttcagaa ggcggaaacg cagtccatat atatcactat gcgcgggggc
     7621 gcgatgagaa cgaagtccgc aatgttatgg tgaagcccgg agacatcatc agagtgaagc
     7681 gcgccggcat tgtctatgtt ctcggtgccg tcaaccgtcc gggtggatat gtaatgcagg
     7741 aagatggcgc gctggacgtg gcgcaggcca tctccatggc aatgggtacg accatgcagg
     7801 caaagattgg cggcattcgc gtagtccggc acgatcaaaa tggcaatatc actgagattc
     7861 cggtcgccta taaagacatt gtgggcggaa aagaaaaacc ccttcaactg gaggcccagg
     7921 acatcgtgta tgttcctgtc agcaagctga aatctgtttt cactgccgga tcaacgatcg
     7981 taggccagac aggtgcggcc accatctacg cagtcaaata aggccagcga ttcatgttga
     8041 attatgtgaa cacagaaagc gaacaggtct ctatttatcc atgggacata gaatcctggc
     8101 gttcagccat gcggattgcc ggcattcccg gacttgcgct gcttagttta ttgctgggca
     8161 caatgattca gtatgcggac ccttcaagtg gaggccccgc acatacaagc accacgatgt
     8221 tggtcctggt gcgcggtccg ctatgtgtgc tcgcatttct gctactgctt tttcagccgc
     8281 atgcgccgcg ccttcgcctg aaggatacgc gtgttctgtt ttttgccttt gccattctct
     8341 atctggtttc cactttatgg tcaacagagc ataaaggcac attaggtaaa tctgtggaga
     8401 tctttctggc gggggtggtc ttctgggaag tcagccgcac aagaaatgct ttgcaacgcg
     8461 tagaggcact taggcagatt attttattaa ctatatcgct tgtagcattt gttacagtca
     8521 ctggatactt cctgcacatt cacgccttta tccagccgcg gcccggcatc tttaccagga
     8581 ctactgcaca ggcgccattt ctttccggga atggattggg atatgtttcc agcgcgctgt
     8641 ttctggttgt atttgcagaa tggcaggcca aaagactgga ctgcggcccg gcacttcgcc
     8701 agatgacgtt cgcgatgttt atgttcgctt ttgctgcgag tcgaacgtct tttgccattc
     8761 ttgttcttgg tgtgattttt gttgtcgcca gaaaatcaag ttcactcggt tcgttcctgg
     8821 cagcagccac ttttgctctg ggactgacat tccaggacaa catcatggtg gcgctgcaag
     8881 ggaaggaggc aacaagcgac tttacaaccc tcagtggcag gacagtcatg tggactgctg
     8941 ccattcgaca atggcaaaag cacccgcttt taggcgccgg aggcggtgta ggaggaaagg
     9001 aaactctgaa gcatatcgga aacttttcac tacaaactgc aagctcttta cataacggat
     9061 ttatggagct gctgaccggt ctgggagcag ttggttttgc cttgggagtg tttcttttgg
     9121 tgctggtgac attgcgaaca tggaaggcat ggcgggagaa tccgcaatat gccggaacat
     9181 atgtgctgat cattcacgtc tggatgacta cggtgatgtc aacaggcgtc ctgggctgga
     9241 tgggatatga gatggccctg ttcctttgca tcatgacgaa ccttgatctt gtccggcacc
     9301 ggcctgccta tgcgtaccag cgtgtggtc
//
LOCUS       DASRBV010000002         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_456146, whole genome shotgun sequence.
ACCESSION   DASRBV010000002 DASRBV010000000
VERSION     DASRBV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456146"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFA02_00040"
     CDS             <1..413
                     /locus_tag="VFA02_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08262.1"
                     /translation="PSIIPWPSGPPRCGHTLSMTEIAPAALATHSVLSPATISRASPA
                     GGSSDFAQILTNSGMCFPNLLSFDSHATLIFRMPEPPSSNPAPGKEEGKDVRLWCPNC
                     GREVNDPLVCGDCSAVICRVCGTPLESADELAFG"
     gene            complement(410..1933)
                     /locus_tag="VFA02_00045"
     CDS             complement(410..1933)
                     /locus_tag="VFA02_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HZU08263.1"
                     /translation="MKTKRSLLLFATLLFVSFLFSRAEVRLPNVISSHAVLQRNRPIH
                     IWGWASPGETVTVRFHGQSRAATADDLGKWSLWLAPESAGGPYNLTAQGSGGAPVTLT
                     DILVGDVWLASGQSNMEMPLNGFPGSAVVKNGAQEIAKANLPQVRLLRLEKKSSLYPQ
                     TDVSATWTECTAQTAAEFSAVAYFFGREINQREHVPIGLIDSTWGGTPVEAWISMDTI
                     GSNAGMMPVFSYWAQFTDDLADRDLELAREKRQDEAAAQAGQPKPKHPWHPDGISWQP
                     AGLYNGMIAPLVPYTIQGVIWYQGETNSNPVRAPYYARFFRALISDWRTHWQEGNFPF
                     LYVQISNFYSPNEDWGMVRDAQRRTLSVANTAMAVTLDIGDRDNVHPADKQTVGSRLA
                     LAARALVYGEPQLEYSGPLFRTATVEGPAMRVWFDHAKDGLAVHGPQPEGFELAGEDG
                     KFVPATAKIDGQTVVVEASGVKHPTAVRYGWASYTTANLFNKEGLPASTFTSVEKPD"
     gene            complement(1988..2383)
                     /locus_tag="VFA02_00050"
     CDS             complement(1988..2383)
                     /locus_tag="VFA02_00050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HZU08264.1"
                     /translation="MVRYVVAALILRDGKVLICQRRPDQAMALKWEFPGGKMEPGEST
                     EEALVRELKEELGIDAEIGPQIAHIRHTYRNGGAVDLQFFAVHTFTGEITNHIFNDLR
                     WCRLTDLPKYDFLAADRGLVQDLAAGKLL"
     gene            complement(2421..>3131)
                     /locus_tag="VFA02_00055"
     CDS             complement(2421..>3131)
                     /locus_tag="VFA02_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU08265.1"
                     /translation="RSRNLAVVHDSWTTTTDELTALAPDLVVASVPYRQESLAAILKA
                     GFPVLALAPHSLADIYNDIRLIASVANAREQGETVISEMQSAIEEIRRKASSALHRPL
                     VYCEEWGKPLIHSQLWVKELVEIAGGQFLGKPGSVTDAETIAAANPSVILMAWCGAGH
                     RVPLERAVEKRGWQALDAVQQGRVFCIADELLNTPAPTLTEGLRAIASAIHPAIFGEM
                     ECNAGRRIEFQPAASKLK"
BASE COUNT          636 a          873 c          943 g          679 t
ORIGIN      
        1 cgccttcaat cattccctgg ccgagcggcc ctccgcgatg cgggcatacg ttgtccatga
       61 ccgagattgc tccggccgcg ttggcgacac acagtgtcct gtcgccagcg acgatctcgc
      121 gtgcctcccc ggcgggagga agctctgatt ttgcacaaat tcttacaaat tccggcatgt
      181 gttttcctaa tctgctttca tttgattctc atgctacgct catcttcagg atgcctgagc
      241 caccgtcatc gaatccagct cccgggaaag aagaaggcaa agatgttcgc ctgtggtgcc
      301 ccaactgcgg gcgcgaggta aacgacccgc tggtgtgcgg ggactgctcg gcagttatct
      361 gccgcgtgtg cggaacaccg cttgaatccg ccgatgagct ggcgttcggc taatcgggct
      421 tctccacaga agtaaaggtg gatgccggca gcccttcttt gttaaagaga ttcgcggtgg
      481 tatagctggc ccatccatag cgaacggccg tggggtgctt gacgccagat gcttcaacca
      541 ctaccgtctg accgtcaatc tttgctgttg cggggacaaa ttttccgtct tctccggcga
      601 gttcaaagcc ttccggttga gggccgtgaa cagccagtcc atcttttgcg tgatcgaacc
      661 agacgcgcat ggccgggcct tccacggtgg cggtgcggaa gagcgggccg gaatactcaa
      721 gctgcggttc gccatacacc agcgcacgtg ctgcgagcgc caggcgggaa cccacggtct
      781 gcttgtcagc cggatgcacg ttgtcgcggt cgccgatgtc gagagtcaca gccatggcag
      841 tgttggcgac agagagcgtg cgccgctggg cgtcgcgcac catgccccaa tcttcattcg
      901 gcgaatagaa gttggaaatc tgaacgtaga gaaaggggaa attaccttcc tgccagtgtg
      961 tgcgccagtc ggaaatgagt gcgcggaaaa atcgcgcata atacggcgcg cggacaggat
     1021 tgctgtttgt ctcgccctga taccagatga ctccctggat ggtgtagggg accaggggag
     1081 caatcattcc gttataaagc ccagcaggct gccatgagat tccatcggga tgccatgggt
     1141 gctttggctt tggctgtcca gcctgcgccg cggcttcgtc ctgtcgcttc tctctggcca
     1201 gttccagatc gcggtcggcc aggtcatcag taaactgcgc ccagtaggag aagacaggca
     1261 tcatgccagc gttggagccg atggtgtcca tgctgatcca ggcctcgacc ggagtaccgc
     1321 cccaggtgga atcaatgagg ccgatgggca catgctcgcg ctggttgatt tctctgccga
     1381 agaaataagc cacagcggaa aattccgcag cggtttgagc agtgcactct gtccaggtgg
     1441 cggaaacatc ggtctgagga tagagcgagc ttttcttttc aaggcgcagc aatcgtactt
     1501 gcggcaggtt tgctttggcg atttcctgtg caccgttttt gaccacggcg cttccgggaa
     1561 agccgttcag cggcatttcc atattcgatt gaccggaggc aagccagaca tcgcctacaa
     1621 gaatgtctgt caacgtgacg ggagcgcctc cgctgccctg ggccgtcagg ttgtatgggc
     1681 cgccagcgct ttcgggagcg agccacaggc tccattttcc gagatcgtca gcggttgccg
     1741 cacgcgactg gccgtgaaac cgcaccgtaa ctgtttctcc cggactggcc catccccaga
     1801 tatgaatggg cctgttgcgc tgcaggaccg catggctgct gatgacgttt ggcagacgga
     1861 cttctgcccg ggagaaaaga aaagatacaa agagaagtgt ggcgaataaa aggaggcttc
     1921 gtttggtctt cataggctgg aacaaccata gcatttgaag aagcgcgtgc gcgcggaaca
     1981 gacgtagtta tagtaatttt ccggctgcca ggtcctggac gagtccacgg tcagcggcga
     2041 ggaagtcata tttcggaagg tctgtaagcc ggcaccagcg gaggtcgtta aagatatggt
     2101 tggtgatttc gcctgtgaac gtatgcacgg cgaaaaactg caagtctacc gcgccgccgt
     2161 tgcgataggt atgacgtatg tgggcaatct gcgggccgat ttcggcatca attcccagtt
     2221 cttctttcag ctcgcgaacg agtgcttctt ccgtgctctc gcccggttcc attttgccgc
     2281 ccgggaactc ccatttgagc gccatggcct ggtcggggcg gcgctggcag atgagcacct
     2341 tgccgtcgcg tagaataagc gcggcaacaa cataacggac caaggcagcc gtttctctgg
     2401 aatgtgtacc agtccccacg ctattttagt ttagacgctg cgggctgaaa ttcgatgcgc
     2461 cttcctgcat tgcactccat ctcaccaaaa attgcgggat gaatggccga tgcgatcgcc
     2521 cgcaagcctt ccgtcagagt aggtgcaggt gtgttcagca gctcatcggc aatgcagaaa
     2581 acacgccctt gctgaacagc gtccagcgct tgccagcctc gcttctcaac tgcccgttcc
     2641 aggggaacgc ggtgtccggc accgcaccag gccatgagga tgacgctggg atttgcggcc
     2701 gcgatggttt cagcatccgt taccgagcct ggcttgccga gaaactgtcc acctgcaatc
     2761 tccaccagtt ctttgaccca gagttgcgaa tgaatcagcg gcttgcccca ttcctcgcaa
     2821 tagacgagcg ggcgatggag ggcagaggag gctttccggc gtatttcttc gatagcggac
     2881 tgcatttccg aaatgacggt ctcgccctgc tcgcgcgcgt tggcaacaga ggcaatgagg
     2941 cgaatgtcgt tatagatgtc ggccagcgaa tgcggggcga gcgccagcac cggaaagcct
     3001 gctttgagaa tggcggcgag cgattcctga cggtagggga cagaggcgac caccaggtcg
     3061 ggtgcgagcg cggtcaattc atctgttgtc gtcgtccatg aatcatggac gacagcaaga
     3121 ttgcggctgc g
//
LOCUS       DASRBV010000003         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_253551, whole genome shotgun sequence.
ACCESSION   DASRBV010000003 DASRBV010000000
VERSION     DASRBV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253551"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFA02_00060"
     CDS             complement(<1..767)
                     /locus_tag="VFA02_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08266.1"
                     /translation="MKKLPRLLASAAIVLFAPSFVFAQGPVQPSSGDKVEYFSLQHHT
                     PDQMDPADAAVLEKHQKKVFAAAEIYGYDPSPRSWTSEEVVCPFIPDYILMRYSSTNA
                     AGAESIFTALIPRRKGRVRIVPVLNNGAARFKPAPLDPRNFQLFSEVVPPDLAKKNSN
                     QDGQWLTLSACYAEMTGARSEIPKNPSADIHMITVPPPTLRITVGGKEQEASFAEPLS
                     KTGYRLWNITYNDAGRIIAVSDDQHSYGEPVVLHPSEP"
     gene            893..1711
                     /locus_tag="VFA02_00065"
     CDS             893..1711
                     /locus_tag="VFA02_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU08267.1"
                     /translation="MGKLLEIDQLSISFPARNARPAVDRLTAGIAPGEVLGLVGESGS
                     GKSVTALAILGLLDATARVEGSVRFDGRDLLRLSREEMRRLRGREISMIFQEPMTALN
                     PVMTVGEQIAEAVRTHHPELGRRQVREAVIEAMHAVAFPGPESRMRDYPHQFSGGQRQ
                     RIMIAMAVVNRPRLLIADEPTTALDVTVQAQILELLGGLQKRYGLSMLFISHDLAVVS
                     QIADRVAVMRHGVLLEEGTKAAIFHSPQHAYTRSLLGAVPTLQTDLSRPLATLQ"
     gene            1764..2516
                     /locus_tag="VFA02_00070"
     CDS             1764..2516
                     /locus_tag="VFA02_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZU08268.1"
                     /translation="MRALILSDIHANLEALEAVLAAAPGHDVVWNLGDVVGYGASPNE
                     VIDRVRALGNVFVRGNHDRVCSGLPGIDDFNPIAGRAARWTQCVLSAEHIAWLRQLAA
                     GPVMPDGPNVACTHGSPLDEDEYILSMRDAWQPLETVKTRINFFGHTHLQGGFATNGE
                     EWFKLVPDYATYNEAEEYEIQLRDGVRYLLNPGSVGQPRDGDWRPAFAIYDDAQGTVT
                     FYRVPYDVRLAQMRILRAGLPDRLATRLRDGR"
BASE COUNT          538 a          689 c          839 g          609 t
ORIGIN      
        1 ggttcagaag gatgcagtac gaccggctcg ccgtatgagt gctgatcatc gctgacggcg
       61 ataatacggc ctgcatcgtt gtaggtgatg ttccagagcc tgtagcccgt ttttgaaagc
      121 ggctcggcaa acgaggcctc ctgctctttg ccgccgacgg tgatgcgcaa tgttggaggc
      181 ggcaccgtga tcatgtgaat gtcggcgctg ggattttttg ggatttcaga ccgtgcaccc
      241 gtcatttccg cgtagcaggc cgagagagtc agccattgac catcctggtt gctgttcttc
      301 tttgccaggt caggcggaac tacttcgctg aacaactgaa aatttctcgg gtccagcgga
      361 gcaggtttga agcgcgccgc gccattgttc agcacaggaa cgatgcggac acgtcccttc
      421 cggcggggaa tgagggcggt gaagatggat tcggccccgg cggcattcgt actggaatag
      481 cgcatgagga tgtagtccgg aatgaaaggg cacacgacct cctcagacgt ccaggagcgc
      541 ggagacgggt catagccgta aatctctgct gccgcaaaaa ccttcttctg gtgtttttca
      601 agaacggccg catcggcggg atccatctgg tcaggagtat ggtgctggag cgagaaatac
      661 tcgaccttgt cgccagacga gggttgcaca gggccttgag caaaaacaaa tgaaggagca
      721 aaaagaacaa tggctgctga ggcaagcagg cggggaagtt tcttcatgcg cttttacttt
      781 accattcccg gggaacggcc caaagggttt attgcccggg gccgacgccg cgaaagtggt
      841 cctttaagcg atcacccggt gttctggttg tctatgccgt tatcatgctt gtgtgggcaa
      901 gcttcttgag atagaccagc tctccatttc atttcctgcg cggaatgcgc gtccggcggt
      961 agaccggctg acggccggca ttgcgcctgg agaagtgttg gggttggtgg gcgagtcggg
     1021 ttccgggaag tcggtgacag cgcttgccat tctgggattg ctggatgcca cagcgcgtgt
     1081 tgaggggtct gtccggttcg atggacgcga tctgctgagg ctaagtcgtg aagagatgcg
     1141 gcgtctgcgc gggcgcgaaa tttccatgat ttttcaggag ccgatgacgg cgctgaatcc
     1201 tgtgatgacg gtaggtgagc agattgcaga ggcggtgcgg acgcaccatc cggaactggg
     1261 ccgccggcag gtgcgcgagg ccgtgataga ggcgatgcac gctgtggcat tccctgggcc
     1321 ggagagcagg atgcgggact acccacatca attttccggc gggcagcgcc agcgcatcat
     1381 gattgcaatg gccgttgtga accgtccgcg tctgctgatt gccgatgagc caacgacggc
     1441 actcgatgtg accgtgcagg cgcagattct ggaactgctt ggcggtctgc aaaagcgcta
     1501 cgggctttcc atgctattta tctcgcatga tcttgccgtg gtttcgcaga ttgctgaccg
     1561 cgtcgcggtg atgcggcatg gtgtgctgct tgaagaaggc acaaaggccg ccatttttca
     1621 ctcgccgcaa catgcgtaca cgcgcagtct tctgggtgct gtacccacgt tgcagacaga
     1681 cctctccaga ccattggcta cactgcaata aaactgtgcc gggtgctggc cgtgtgacag
     1741 aagcatctat atttattgga aatatgcgcg cactgattct ctccgatatc cacgccaatc
     1801 ttgaggccct ggaggccgtt ctggcggctg ctccgggaca cgatgtggtg tggaacctgg
     1861 gtgatgtggt gggttatggg gcaagtccga atgaggtgat agaccgcgtg cgcgcgttgg
     1921 gaaacgtatt tgtgcgcgga aaccatgacc gggtttgcag cggactgccg ggaattgatg
     1981 acttcaatcc gatagccggg cgcgccgccc gatggacgca atgcgtgctt tcagcagaac
     2041 acatcgcatg gctgcgtcag cttgcggccg gccctgtgat gccggacggc ccaaatgtgg
     2101 cctgcacgca tggatcacct ctggatgaag atgagtacat tctttctatg cgtgacgcct
     2161 ggcagccact tgaaacagta aagacacgca ttaatttctt cggccacaca catctgcagg
     2221 gcggctttgc cacgaacggc gaggagtggt ttaagctggt ccctgactac gcaacatata
     2281 acgaggcgga ggagtacgaa atccagcttc gtgatggtgt gcggtacctg ctgaatcccg
     2341 ggtcggtggg gcaaccgcgc gatggagact ggcgtccagc ctttgcaatt tacgatgatg
     2401 cgcagggcac ggtgaccttc taccgggtgc cctacgatgt gcgccttgcg cagatgcgca
     2461 ttcttcgcgc tggattgccc gaccgcctgg cgacgcgctt gcgcgatggg cgatgagctg
     2521 tatcttgaat cactgatttt tatcggtgat gtttcccacg aaaatttcag gcatactgaa
     2581 agtacatgca agccttatat ccattcagac ttgctcctta ttttcggacc cgcatctggg
     2641 ggttccacga cctttcaccg tggtttgatt acaaa
//
LOCUS       DASRBV010000004         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_557620, whole genome shotgun sequence.
ACCESSION   DASRBV010000004 DASRBV010000000
VERSION     DASRBV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557620"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFA02_00075"
     CDS             <1..990
                     /locus_tag="VFA02_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZU08269.1"
                     /translation="GNVVDPVDIANRLGAEIVRLWVASVDFREDVSASENLMQRIADN
                     YRKLRNTFRFLLGNLHGFHPAEHAVPYEDLLPLDQYMLARTRELVEKVCGTDGKGGWY
                     GSFEFHRAYHAINEFCIVDLSATYLDVLKDRMYTFAPASMARRSAQTVLWKIAEALVR
                     MVAPVLSFTADEVWQYLPQVEGRPVSVHLTKFPTPDEIAPAAPGLLTEWEKLFAVRDE
                     ALKSLEEARKEKRIGKALEAKVRITADAETMAVLEKYESSLKELFNVSQVETGKHVTD
                     AVEAVTLPADGTKCERCWNYMPDVAAFGPWARVCGRCATALEQMGYKQTVLSS"
     gene            1054..1554
                     /gene="lspA"
                     /locus_tag="VFA02_00080"
     CDS             1054..1554
                     /gene="lspA"
                     /locus_tag="VFA02_00080"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZU08270.1"
                     /translation="MNKDRRGWLLLIAAFIIAFDRWTKILVTRHIPEGDLKSIIPGWL
                     WISHVLNPGAAFSLFADSASPEHTRWVLVGFSVLVAFAVFAFLVARVRRFSVTNFALA
                     LVLGGAIGNLWDRIRYGVVTDFLLVQLHFGHWSYHWPDFNVADSAITCGAVLVLLTSL
                     WPQRTS"
     gene            1713..4640
                     /gene="uvrA"
                     /locus_tag="VFA02_00085"
     CDS             1713..4640
                     /gene="uvrA"
                     /locus_tag="VFA02_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZU08271.1"
                     /translation="MTDQIIIRGARVHNLKNIDCDIPHGKLTVVSGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLERIEKPDADLIDGLAPAIAIKQKNSTRNPRSTVATA
                     TEIYDYMRLLYARCGTVYCTVCNGVVKRDTVDEIAAAVLALEEGTRLHALFPVQRAQA
                     QPVSPRTEEKPKRGRKTSAKKTEESPLTDALKERLTDLRKRGFNRLYQNGNIYEFSTP
                     ESLLEINFTLPVYVLVDRIVVSADNRARIVDAAEIGYRESGEIIFEIVLRDSESIERE
                     RLRFSGAFECKNCHRPYKEPEPRLFSFNNPFGACPRCQGFGNTIDFDLDLIIPDKSKT
                     LDEGAIDPWNRPKYRAYFTELKRAAKQHGIPMDVPWHDLTPDQQAFVLEGRGDFLGVH
                     GFFEYLERKKYKLHVRVMLSKYRGYALCPECKGQRLRAEARAVRIAGKNICETAALTI
                     SEANRFFSELTLTPMQEEIAGSVLKEVRQRLHFLDAVGLDYLTLDRLASTLSGGESQR
                     IQLATSLGSQLVGALYVLDEPSIGLHTRDTAKLIRILEDLRDLGNTILVVEHDPDVVR
                     AADFLLDLGPGAGEFGGRVLAAGAVEDVEHNPDSLTGRYLSGQLSIPVPTRRREPGKE
                     WLRLRGARSHNLKGIDVDIPLNMLVCITGVSGSGKSTLVHEVLYRAVAHALGKGEGAD
                     PTNLYKELKGVERLNDIVLVDQNPIGRTPRSNPVTYIKAFDAIRELFAALPEAQRRGY
                     SAGHFSFNVPGGRCDVCEGDGTVTVEMQFLADVELPCEECHGTRYKASTLEIKYKGKS
                     IHEVLNLTVKEALRFFSGQQKIQEKLAVLDEVGLGYVRLGQSATTLSGGEAQRVKLAA
                     HLGSVRAGGKSRVLYVLDEPTTGLHFDDVSKLLTAFRKLIDGGGSLLVIEHNLDVIKS
                     ADWVIDLGPEGGAKGGQIVATGTPEEITRNELSHTGYWLAKVLSPRGSARPREEAESE
                     VAV"
     gene            4763..>5776
                     /locus_tag="VFA02_00090"
     CDS             4763..>5776
                     /locus_tag="VFA02_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08272.1"
                     /translation="MTCSAGFAQSAPLPPGTSLDAPSKDSGPLAKAEDAIASKDFDQA
                     KNLLDPYLSAHPSDARALFDRGFIEDSQERDDLAAGYYEKAIAADPKQFESRLALGLI
                     LARQGKPQEAREQLKAATQLGPNPPNPAAKAQAFRALAQLDRNSDPDAAKQALIQALQ
                     ISPETPDDILLTGEIAEAEGDMASAETAYRRALQKQPESSAAAAGLAHVLIAQKKYPD
                     AEPLIKAALSRDPDDPALNTQLALVLNAEGKQDEAVGVLEKLHQLKPDDASISKMLAD
                     AYAQGGQADKADLLYTQLLKASPDDPALLAAHGDSLVRQQKYAEALAVLQRAVQLRPD
                     DGNA"
BASE COUNT         1230 a         1650 c         1747 g         1149 t
ORIGIN      
        1 ggcaacgtag tagatccggt ggacatcgcc aaccgtctcg gcgctgaaat cgtccggctc
       61 tgggtggcgt cggtggattt ccgcgaggac gtctccgcca gcgaaaacct gatgcagcgc
      121 attgcggaca actatcgcaa gctgcgcaac accttccgct ttctgctcgg aaacctgcac
      181 ggctttcatc ctgctgagca cgcggtgccg tatgaagacc tgcttccgct tgaccagtac
      241 atgctggcgc gcacacggga gctggtagag aaagtctgcg gtacggacgg caaaggcgga
      301 tggtacggca gcttcgagtt ccatcgcgcc taccacgcca tcaacgagtt ctgtatcgta
      361 gacctgagcg ccacctatct ggacgtgctc aaagaccgca tgtacacctt cgcgcctgca
      421 agcatggcgc gccgctccgc acagactgtg ctgtggaaga tcgccgaggc gctggtgagg
      481 atggttgcgc cagtcctgag cttcacggcg gatgaggtgt ggcagtattt accgcaggtg
      541 gaggggcgtc cggtaagcgt gcatctgacg aagtttccca cgccggacga aattgctccg
      601 gctgcgccag gattgcttac cgagtgggaa aagctctttg ctgtccgcga cgaggcgctg
      661 aagagcctgg aagaagcacg gaaagagaag cgtattggaa aggcgctcga agccaaagtc
      721 cgcattacag cagatgccga aacaatggcg gtccttgaaa aatatgaatc gagcctgaag
      781 gagttgttca acgtatctca ggttgaaaca ggaaagcatg tgacggatgc tgttgaagcg
      841 gtcacgcttc cggccgatgg tacaaaatgc gagcgttgct ggaactatat gccggatgta
      901 gcagcttttg ggccttgggc cagggtttgc ggacgttgcg caaccgccct cgaacagatg
      961 gggtataagc agaccgtgct gtcgtcgtaa ggagaatatc catagcgaag cagcgagaag
     1021 actgacattg gtcaggggag caaagaggtc caattgaaca aagaccgacg cggctggctt
     1081 ctgcttatcg ccgcctttat catcgcgttc gaccgctgga cgaaaatcct cgtcacgcgc
     1141 cacattcccg agggagactt gaagtccatc atcccaggat ggttgtggat ctcccacgtc
     1201 cttaacccgg gagcagcatt cagcctcttc gccgattcgg cctcgccgga acacacgcgc
     1261 tgggtccttg tcggattttc tgttcttgtc gccttcgcgg tctttgcctt tctggtggcc
     1321 agggtccgcc gctttagtgt gaccaatttt gccctggcac ttgttttagg cggggccatc
     1381 ggcaatctct gggaccgcat ccgctacggt gtggtcacag attttcttct cgtccagcta
     1441 cacttcggcc actggagtta ccattggccc gacttcaatg ttgccgactc agccattacc
     1501 tgcggcgctg ttctggttct gctgacttct ctgtggcccc agaggacttc gtaatattga
     1561 gttccctctc ctgtctgttt tttgtaaatt cttcaaaagg cgtgagttaa gaaggcattt
     1621 ttggggttga cggcgcgggg cgcggtcccc tggagcaaca ccgtataatc aaactgggaa
     1681 gtcttacaca aaactctccg gggcgcacag agttgaccga ccagatcatt atccgcgggg
     1741 cgcgggtcca caacctgaaa aacattgact gcgacattcc gcacggaaag ctcacggtcg
     1801 tttccggcgt ctccgggtca gggaagtctt cgcttgcctt cgataccatc tatgccgaag
     1861 gccagcggcg ctatgtcgag tcgctctcgg cctacgcacg ccagtttctg gagcgcattg
     1921 aaaagccgga cgcagacctg attgacggtc tggcgccagc cattgccatc aagcagaaga
     1981 actcgacccg taatccgcgc tccacggtag cgacggcgac ggaaatctac gattacatgc
     2041 gtctgcttta tgcgcgctgc ggaacggtct actgcaccgt ctgcaacggt gtggtgaaac
     2101 gtgacactgt agatgagatt gccgcggcgg tgctggcgct ggaagaaggc acgcgactcc
     2161 acgcgctctt tccggtgcag agagcgcagg cgcagcccgt ctctcccagg acggaagaaa
     2221 agccgaagcg tggccgcaag acttccgcga agaagacgga agaatccccg ctgacggatg
     2281 ctcttaaaga gagattgacc gacctgagga agcgcggctt caaccgtctt tatcagaacg
     2341 gcaacatcta cgaattttct acgcccgagt ctctgctcga aatcaacttc actctgccgg
     2401 tctacgtatt ggtggaccgc atcgtggtca gcgcagacaa ccgcgcccgc attgtggatg
     2461 cggcggagat cggctatcgc gagtcgggcg aaatcatctt cgagattgtt ctgcgtgatt
     2521 ctgaatccat cgaaagggag cgactgcgct tctccggtgc attcgaatgc aagaactgcc
     2581 atcggcctta caaggaacca gagccgcgcc ttttttcctt taacaatccg tttggagcgt
     2641 gtccacgctg ccagggcttt ggcaacacga ttgatttcga ccttgacctc atcattccgg
     2701 acaaatcaaa gacactcgac gaaggcgcga ttgacccctg gaaccgcccg aagtaccgcg
     2761 cctactttac tgagctgaag cgcgcggcaa aacagcacgg tattcctatg gacgtcccct
     2821 ggcatgacct gacgcctgac cagcaggcat ttgtgctcga aggccgcggc gactttctgg
     2881 gcgtccacgg attttttgag tatctggagc gcaagaagta caagctgcac gtccgcgtga
     2941 tgctgagcaa gtatcgcgga tacgcgctct gccccgagtg caaaggccag cgcctccggg
     3001 cggaggcgcg cgctgtgcgt attgcgggca aaaacatctg cgagaccgcc gcactgacga
     3061 tcagcgaagc gaaccgcttc ttctcagagc tgacgctcac gccgatgcag gaagagatcg
     3121 ccggcagcgt gctcaaggag gtccgccagc gcctgcattt tctggatgct gtgggcctcg
     3181 attatctgac gctcgaccgt cttgcctcga ccctttccgg tggagaatcg cagcgcattc
     3241 agcttgccac ctcgctcggc tcgcaactgg tgggcgcgct ctatgttctg gatgagccgt
     3301 ccatcgggct gcacacgcgg gacacggcga agctcattcg cattcttgaa gacctgcgcg
     3361 atctgggaaa cacgatcctg gtggtcgagc atgatcccga cgtggttcgc gcagcggact
     3421 ttctgcttga cctcgggccg ggcgcaggtg aatttggcgg gcgggtgctg gctgccgggg
     3481 cggtggagga tgtggaacac aatcctgatt cgctgactgg aagatatctc agcgggcagc
     3541 tttcgattcc cgttcccacg cgccgccgcg agccgggaaa agaatggctc cgcctgcgcg
     3601 gcgcgcgatc acacaacctc aaggggattg acgtagacat cccgctgaat atgctcgtct
     3661 gcattacggg tgtttccggt tcgggcaagt ccacgctggt gcatgaggtg ctttatcgcg
     3721 ctgtggcgca cgcgctgggc aagggcgaag gcgcggaccc gacaaacctc tacaaagagc
     3781 tgaagggcgt ggagcggctg aacgacattg ttctggtaga ccagaacccc atcgggcgca
     3841 cgccgcgctc gaacccagtg acatacatca aggcatttga tgctatccgg gaactttttg
     3901 ctgcgctgcc agaggcgcag cgccgcggct actctgccgg gcacttctct ttcaatgtcc
     3961 caggcgggcg ctgcgatgtc tgcgaaggcg atggtacggt cacggtcgag atgcagtttc
     4021 tggcagacgt ggagctgccc tgcgaggagt gccacggaac ccgctacaag gccagcactc
     4081 tggagatcaa gtacaagggc aagagtatcc acgaagtgct gaatcttaca gtaaaggaag
     4141 cgctgcgctt cttctctgga cagcagaaga tacaggaaaa acttgcagta ttggatgaag
     4201 tcgggctggg gtatgtccgg ctgggccagt ccgcgactac gctctccggc ggcgaggccc
     4261 agcgggtgaa gctggcagcg catctgggaa gcgtacgcgc cgggggcaaa agccgtgtgc
     4321 tctatgttct ggatgagcct acgaccgggc tgcacttcga tgacgtgagc aagctgctga
     4381 cggcattccg caagctcata gacggtggcg gttcacttct ggtcatcgaa cacaatctgg
     4441 acgtgatcaa gtccgcagac tgggtgattg acctggggcc tgagggcggt gcaaagggtg
     4501 ggcagattgt ggccaccggg actccggaag agattacacg aaatgagctt tcgcataccg
     4561 gatactggct ggcgaaggtg ctgtcgccgc ggggaagcgc gcgtccaaga gaagaagcag
     4621 aaagcgaagt agcggtatga agaagattct tgctccctca acgtttcata aagagcgcga
     4681 aaggacgggg tacccagaag gaaagagcag atccttcatc gcaccgctcc tcaggatgac
     4741 aatgttaggg tcagggctgc ttttgacttg tagtgctggg ttcgcccagt cggccccgct
     4801 gccgccgggg acaagcctgg acgcaccttc caaagattca ggcccgctgg ctaaggccga
     4861 agacgccatt gccagcaaag attttgacca ggcaaaaaac ctgctcgacc cgtatctttc
     4921 cgcacacccc agcgatgccc gtgccctgtt cgaccgcgga tttattgaag actctcagga
     4981 acgggacgat ttggcggctg gatactatga gaaggcgatt gcagccgacc caaagcagtt
     5041 tgagtcacgc ctggcacttg gcctgattct ggcccggcag ggcaagccgc aggaggcgcg
     5101 tgagcaactg aaggcggcca cgcagcttgg gcccaacccg cccaatcctg cggcgaaggc
     5161 gcaggccttt cgggcgcttg cccagcttga ccgcaatagc gaccccgatg cggcgaaaca
     5221 ggcgctgatc caggccctcc agatcagccc ggaaacgccc gatgacatcc ttttgacagg
     5281 agaaattgcc gaggcggaag gcgatatggc ctcggcagaa acggcttacc ggcgtgccct
     5341 ccagaagcag ccggagtctt cagccgccgc cgccggactt gcccatgtgc tcatcgcgca
     5401 gaaaaaatac cctgatgccg aaccgttgat caaagcggcg ctcagccgcg accctgacga
     5461 tccggcattg aatacgcagc ttgctcttgt gctgaatgcc gaaggcaagc aggacgaggc
     5521 tgttggcgtg ctcgaaaaac tgcatcagct taagccagac gacgcttcca tcagcaagat
     5581 gctggccgat gcttacgcgc agggcggtca ggcagacaag gccgacctgc tctacacgca
     5641 gcttctgaag gcatcgccgg atgatcctgc ccttcttgcg gcgcacggag acagcctggt
     5701 gcgtcaacag aagtacgccg aggccctggc ggtcctgcaa agggcagtcc agctcaggcc
     5761 ggatgacgga aacgcc
//
LOCUS       DASRBV010000005         8482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_152286, whole genome shotgun sequence.
ACCESSION   DASRBV010000005 DASRBV010000000
VERSION     DASRBV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152286"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3463
                     /locus_tag="VFA02_00095"
     CDS             <1..3463
                     /locus_tag="VFA02_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08273.1"
                     /translation="QTNDSGAYTAEHLIPDKYDVKVEASGFQSFEAKGIQVFADTSPK
                     VDAQLQVGGASQTVTVNADAIPVLKTDRADVSTEFTTKTITDLPIPDRNFTNIQLLLP
                     GAQPLGWGHAASENPQASKQIQVDGQAFGGVAFQLDGTDNQDPILGIIVINPPLDALS
                     ETKITTQNFDAEFGKAVSSFVMAQTKSGSNNFHGTAYDYRESNANLARDPYTQFQPDP
                     VTGHFLPPGLKNQFGGTLGGPIIKDKLFFFGDYEGIRQKVGISYTATVPTKLLTATCL
                     GQETGPSGIPGCDFSEYPNTIYQQTSSGSKAYPGNVIPTAQLSTQALALLKLLQPYAP
                     NTSGALNGTKNNYAASGTGGFNADQWGQRVDWQATSKTHVFERFSRFTDTLTGKTMFG
                     DAGGPGFGLQGYGGTSKGANDSLAAGMDIAISPTVLTDFRIGYYRYNIVTSKYDQGTD
                     FAHQLGISGLNMGTAVTSGAPAFNIADSDRSGNQTANNAQSQGPQYGSGLNINRCNCP
                     LTEKEDQFQIVNNWTKIIGNHSLKFGADLRYARNLRVPSDTNRAGELRFDPGPTSNNG
                     ETGGLGFASFVLGDVTNFGRYVSVSTNAKEFQKRFFFYGQDTWRVTPNLTLNLGVRYE
                     LYYPESVNAKGNGALMNLKDGYLRVAGYGNVPSNMGWSPASNAWNPRVGLAYQVDPKT
                     VIRAGYGRSFDIGVFGSIFGHVVTQNLPVLANQSITSPTTTSYAFNLNNGPAAYPFPS
                     VPSNGLLPAPGYAVSPKARPNSLRLPTLDAWNLSVQRSLTPTLSVTLAYVGNKGTHTL
                     SAGDGNNTNPNEAGIFLPAQYSVEGRTLHYDPNPKNPDALGIGADGGTNVSNFLSRYY
                     GGTLPACQDPNYANPGGPGVAPGMCGWTNGISYYGDDQDTEFNALQASMAKQFTHGLS
                     FNVNYAWQRGYNFASGYATWDKTAVKGRDDNIREQQIIGYGTWQLPFGRNQWIGSNVP
                     RIVDEFIGGWQFSPVVTWATGLPFTVGYSNCGESIPGSAPCYPNGKAGYIKTNLTKFD
                     PVSHTRTFFSGVTCSDQKTGLTPTSPCGQFTAPGLDQIGNMGRNSVFGPHFFNADLSM
                     QKNFPIYESLLAQFRVDAFNGFNHINAGNPSQTNIDGGPFVITGEPALNVYTNPRQLQ
                     FSLRLQF"
     gene            3568..4518
                     /locus_tag="VFA02_00100"
     CDS             3568..4518
                     /locus_tag="VFA02_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08274.1"
                     /translation="MGGQQQASAPSLDHVRQLWIQGRPNEALAEADTLRRQNPLPAGI
                     DRLRGFILYQQNKLSDADAAFAEAIKQDPKDTEAMEMRGVTLFRMGRAADAIPFLEAA
                     RASVPGANIDPNYVLGVSYLQVHRYDDARHTFAAQYGFAPDSAPAYLLTARMALHQEL
                     VAAAEQSAQKALQLDPRLPGAHLLLGEIALARADSTHAIAELEQERAINPLNGEVYDR
                     LGDAYVRSEQYAKAQQALNCAVLLEPNATGPYILLGKALLGQQNPVMAGTYLERALQM
                     DPGNYIAHSLLAQVYRAEGRKDDAAREFQIAERLQSGAQH"
     gene            4518..6278
                     /locus_tag="VFA02_00105"
     CDS             4518..6278
                     /locus_tag="VFA02_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08275.1"
                     /translation="MLCPTQCRVLQALKRYGLAALMVVNVHLGFAQQSNRLHEADAAF
                     RAGYAAASQGDLATARQQFERVVQLAPQIEEGHSALGSVLYQTGDYPAAIRELEKALA
                     LKPSDKAAQENLALAYTQTGNSQKALEFFVKSGGTDPQILAAWARALAATGKLTEAIE
                     KTQNAITAAPQNAALYDQLGSIYAQIQDWPQAQAAFEHALRIDPQLAAAHLHLGAVFN
                     STQQPQRAIEELTQALQLDPKNAVAHLELGKAFAAVNQDDAAIPHFQRAMTLAPSLTE
                     ATNQLAMALQRNGREQEAIPLFRKVISSTPKNAEALTNLGLALVQTGKAKEAIPCYQR
                     ALAIAPNDATIHQDLGVAYLQQSNIDDSIREFRAGLAMKPNSPELHYNLGLAYKLKDD
                     LVHAIPELETAAKLAPASPDPPYTLGILYMQQGRFDDAVAQFNTALDRRPENGDGWAM
                     LGSVYKQQAKYDAAANALRKAIALLPNQPGPHITLAGVLQEQGKREEAAVERKIAADL
                     TRVAVNRQRAVFATNTGNALLQKDQIADAVARYQEAISSDPAYAEAHRQLAAAYERQG
                     RSADAASERQKAASLDLQKQ"
     gene            6316..8193
                     /locus_tag="VFA02_00110"
     CDS             6316..8193
                     /locus_tag="VFA02_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU08276.1"
                     /translation="MNRRKFLQSLSRTALVLPFSDVLFMATPPWKRAAFFAAQDASQN
                     KPGAQPTYEAKPMPPPPGPKSPIAGTPLGLQFIDVATQSGLNAKTIYGGEGKNKYLLE
                     TTGCGVAFYDYDNDGWLDIFLVNGWRLEGFPKGQEPTCHLFKNNRDGTFTDVTTKAGL
                     ARSGWGQGCCVGDYDNDGHDDLFVSYYGQNALFHNNGDGTFTDVTAKAGLLQAKTRWN
                     SGCAFLDYDRDGHLDLFVANYIDFDLKTAPTPESGPCEYKGILVACGPPGLPGGKNIL
                     YHNNGDGTFTDVSEKAGMWNTIGNYALSVAVADLNNDGWPDIYVANDSTAATLYQNQK
                     DGTFKDIAIEAGAAFSPDGKPQAGMGVSIGDYDRDGNLDIVKTNFAGDTDSLYTNLGD
                     GTFEDHTYLSGLGVNTRYLGWGVGFFDMDNDGWLDLFMSNGHVYPEVSSSKTEAPYAE
                     HKYLYRNLRNGRFEEVTDKAGPGITTPVAARGCAFGDYDNDGDLDIVVNCVNSLPQLL
                     RCDSEWKRNWIKVKTIGTKSNRTGIGARVKVVAKCQPDSQQPLLQIDEIRSGGSYYSQ
                     NDLRLHFGLDQAKMVDLLEIRWPSGQVDAFRNIEANKLYIIEEGSKTPKAISLTGLKK
                     S"
     gene            complement(8171..>8482)
                     /locus_tag="VFA02_00115"
     CDS             complement(8171..>8482)
                     /locus_tag="VFA02_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZU08277.1"
                     /translation="HPTMAFIAGAFICWLIFKSAWKGANRGLAMGVVALLASQYFLGF
                     ADLALLAPTWMQIVHLLGADVLWLALVVLTARVCVIPVGCHESVVPCAKTAELRTSSA
                     Q"
BASE COUNT         2059 a         2532 c         2145 g         1746 t
ORIGIN      
        1 tcaaaccaac gattccggtg catacacggc agagcacctg attccggaca agtacgacgt
       61 gaaagtcgag gcatccgggt tccagtcctt tgaggcaaaa gggattcagg tcttcgccga
      121 cacctcgcca aaagtagacg cccaacttca ggtaggcggc gcttcgcaga ctgtcaccgt
      181 caatgctgac gccatcccgg tgctcaagac tgatcgcgcc gacgtttcta cagaattcac
      241 cacaaagacc atcactgacc taccgattcc agaccgcaac tttacgaata tccagttgtt
      301 gctgccagga gcacagcctc tgggctgggg ccacgcagct tcagagaatc cccaggccag
      361 caaacagatc caggtagacg ggcaggcctt tggcggtgtc gctttccagc ttgacggtac
      421 tgataatcag gacccgattc ttggaatcat cgtcatcaac ccaccgctcg acgcgctttc
      481 tgaaacaaaa atcaccacac agaacttcga tgctgagttt ggcaaagctg tttcgtcctt
      541 cgtgatggcg cagacaaagt ccgggtccaa caacttccac ggcactgcct acgactatcg
      601 cgagagcaac gcaaatcttg cgcgcgatcc ctatacgcag tttcagccgg acccagtcac
      661 aggccacttc ctgcctcctg gattgaaaaa ccagttcggc ggcacgctgg gcggtccaat
      721 cattaaagac aagctcttct tctttggcga ctacgaaggc atccgacaga aggtaggcat
      781 ttcctacaca gcaacagttc caaccaaatt gttgacagcc acctgtctgg gccaggaaac
      841 aggccccagt ggaattcccg gctgcgattt cagtgagtat cccaacacca tttatcagca
      901 aacatcttcc ggatcaaagg cgtatccagg caacgtaatt ccaacagcgc agttgtctac
      961 ccaggcgctt gctttgttga agttgctgca accatacgcg cctaacactt ctggcgcatt
     1021 gaatggaacg aaaaacaact atgctgccag cggaacaggt ggattcaacg ctgatcagtg
     1081 gggccagcgc gtagattggc aggcaacgtc caaaacccac gtctttgaac gcttcagccg
     1141 ctttacggat acattgacgg gcaagaccat gttcggcgat gcaggcggtc ctggatttgg
     1201 acttcagggc tatggcggca cctcaaaagg cgccaatgat agtctcgctg ccggcatgga
     1261 catcgccatc agcccgaccg tgctgaccga ttttcgcatt ggctactacc gctacaacat
     1321 tgttacttcc aaatatgacc agggtacaga tttcgcccat caactcggca tttctggact
     1381 gaacatgggt accgcagtca ctagtggtgc tcccgcattc aatattgccg actctgaccg
     1441 ttctggaaac cagaccgcga acaacgcgca gtcacaagga ccacaatatg gcagcggact
     1501 gaatatcaac cgctgtaact gcccgctgac tgaaaaggaa gaccaattcc agattgtcaa
     1561 caactggacc aaaatcatcg gaaaccactc cttaaagttt ggcgccgacc tgcgctatgc
     1621 acggaatctg cgtgttccca gcgacacgaa ccgtgctggc gagctgcgct ttgatccggg
     1681 cccgacttct aacaatggcg aaacaggcgg ccttggcttc gcttcgttcg tactgggcga
     1741 tgtcacaaat ttcggccgct acgtcagcgt ctccaccaac gccaaagaat tccagaagcg
     1801 cttcttcttc tatgggcagg acacctggcg cgtcaccccc aacctcacgc tgaatctggg
     1861 cgtccgctat gaactctatt atcccgagtc agtcaatgcc aaaggcaatg gcgcgttgat
     1921 gaacctcaag gacggctacc tccgcgttgc cggttacggc aacgtcccaa gcaatatggg
     1981 ctggagcccc gcctccaatg cctggaatcc gcgcgtggga ctggcttatc aggtagatcc
     2041 caagaccgtc atccgcgccg gatatggccg cagctttgac attggtgtct ttggctccat
     2101 tttcggccat gtagtcaccc agaatctccc ggttctggct aatcagtcca ttacatcgcc
     2161 aaccactacc agctacgcct ttaatttgaa caacggcccc gctgcctatc cattcccctc
     2221 agttccatct aatggactgt tacctgcccc gggttatgcg gtcagtccga aagcgcgtcc
     2281 gaacagtctg cgcctgccta cgctggatgc ctggaacctc agcgtccagc ggtcgctcac
     2341 accaacgctc tcagtcactc tggcctatgt tgggaacaag ggcacacata ctctaagtgc
     2401 tggcgacggt aacaacacta atccgaatga ggcaggcatc ttcctacctg cccaatacag
     2461 tgtcgaaggc agaacactcc actatgatcc aaatccgaaa aacccggacg ctctgggaat
     2521 cggcgctgat ggtggaacca acgtctccaa ttttctgtct cgttactatg gcggcacgct
     2581 tccagcttgc caggacccaa actatgcaaa tccaggcgga cctggcgttg cacccggtat
     2641 gtgcggatgg accaatggca tcagctacta tggcgatgac caggacaccg aatttaacgc
     2701 cctgcaggcc agcatggcaa aacaattcac gcacggtctg tccttcaatg tgaactacgc
     2761 atggcagcgt ggttataact tcgccagcgg ctatgccacc tgggacaaga ctgctgtcaa
     2821 aggccgcgat gataacattc gcgagcaaca gatcatcggc tacggcacct ggcagcttcc
     2881 attcggaaga aaccagtgga tcggcagcaa tgttccgcgc atcgtagatg agttcattgg
     2941 tggatggcag ttcagccctg ttgtcacttg ggctacgggt ctgccattta ctgtcgggta
     3001 cagcaactgc ggcgagtcca tcccgggcag cgcgccctgc tatccgaacg gcaaagctgg
     3061 atacattaaa accaacctga caaaatttga ccccgtaagc cacacgcgta cattcttcag
     3121 cggtgtgacc tgctccgacc aaaaaaccgg actgacccca acctcacctt gtggccagtt
     3181 cacggccccg ggcctggatc agattggcaa catgggaagg aacagcgtct tcggtccgca
     3241 tttctttaat gctgacctct cgatgcaaaa gaactttccc atctacgaaa gtcttctggc
     3301 gcagttccgt gtggacgcat tcaacggctt taaccacatc aacgccggga atccctcaca
     3361 gaccaacatt gatggcggcc cattcgtcat taccggcgag cctgcgctca acgtctatac
     3421 caatccgcgt cagctacaat tttcgctgcg attgcagttc tgacgcaacc gtagcatttg
     3481 atgaggggag catttgctcc cctcaattat tttcgagata cacttcattc agtcatgcac
     3541 aaggtctttc ttctgtgtct tctggcaatg ggaggacaac aacaggcttc cgctccatcg
     3601 ctcgatcacg tgcggcagct ttggatccag ggcaggccca atgaggcgct tgcagaagca
     3661 gacactttgc gcaggcaaaa ccctttgcct gcaggaattg accggctgcg tggcttcatc
     3721 ctttatcagc aaaacaagct gtctgatgcc gatgccgcct ttgcggaagc gatcaaacag
     3781 gaccccaaag acactgaagc gatggagatg cgtggcgtga ctcttttccg catgggacgc
     3841 gccgccgacg cgattccttt cctcgaagcc gcgcgcgctt cggttccagg ggccaacatt
     3901 gatcccaatt acgtactggg ggtctcatat ctccaggtac accggtacga tgacgcacgc
     3961 cacaccttcg ccgcgcaata tggattcgcg cccgattccg cccctgccta tctgctgacc
     4021 gcccgcatgg cccttcatca ggaactggtc gcagccgctg aacagtccgc acaaaaagca
     4081 ttgcaactcg atccacgact tcccggtgca cacctgctgc tgggcgaaat cgcgctcgca
     4141 cgcgccgact ctacgcacgc catcgccgaa cttgagcagg agcgcgccat caatcccctg
     4201 aacggtgaag tctatgaccg tcttggggat gcctacgttc gcagcgagca atacgccaaa
     4261 gcacaacagg cgttgaactg cgccgtattg ctggaaccga atgcgactgg accctatatc
     4321 ctgctgggca aagctctgct tgggcaacaa aacccggtga tggcaggcac atatctggag
     4381 cgcgccctgc agatggaccc cggcaattac atcgcgcatt ctctcctggc ccaggtttac
     4441 cgcgcggaag gccgcaagga cgacgctgcg cgagagtttc agattgccga aaggctgcaa
     4501 tcgggagcgc agcattgatg ctttgtccta cacaatgccg agtacttcag gccctgaaaa
     4561 gatatgggct ggctgctttg atggttgtca acgtccatct gggctttgcg caacaaagca
     4621 atcgcctcca tgaggcagac gcggcctttc gtgccgggta cgcagcagca tctcagggag
     4681 atctcgctac agcacggcag caatttgagc gcgttgtaca gcttgctccg cagattgaag
     4741 aaggacatag tgccctgggc agcgtcctct accaaactgg agactatccc gcggccatca
     4801 gggaacttga aaaggcactc gccctgaagc cttccgacaa agcagctcag gagaatctcg
     4861 cgctggcata tacacaaaca ggcaactcgc aaaaggcgct ggagttcttc gtaaaatccg
     4921 gcggcacaga cccgcagata ctcgcagcct gggcgcgcgc ccttgccgcg actgggaagc
     4981 ttaccgaagc catcgaaaaa acgcagaacg ccatcactgc ggcgccgcaa aatgccgcac
     5041 tctatgacca gctcggctcc atctatgcgc agatccagga ctggccgcag gcacaggcgg
     5101 cctttgaaca cgcgctccgg attgatccgc aactcgccgc tgcccatctg catctgggag
     5161 cggtcttcaa cagcacgcag cagccgcagc gggccattga agaactgacg caggccttgc
     5221 agcttgatcc gaaaaatgca gttgcccatc tggaacttgg gaaagcattt gctgcagtaa
     5281 accaggatga tgcagccatc ccacattttc agcgggctat gacactggcc ccttcactca
     5341 cagaagcaac caaccagctt gctatggctc tccagcgcaa tggaagagag caggaagcca
     5401 ttcctttgtt caggaaggtg atttcatcta caccgaagaa tgctgaagca ctcaccaatc
     5461 tgggccttgc gctggtgcag acaggcaaag caaaggaagc aattccctgc tatcagcgtg
     5521 cgctcgccat cgccccaaac gatgcgacga tacaccagga cctcggcgtc gcctaccttc
     5581 agcagagcaa catagatgac tccatccgcg aatttcgtgc cggactcgca atgaaaccaa
     5641 acagccctga gcttcattac aacctgggct tggcctacaa acttaaagat gatcttgtac
     5701 acgcaattcc agagctggaa actgctgcga aactggcccc tgcgtctcct gatcctccat
     5761 acaccctggg cattctttac atgcagcagg gacgctttga tgatgcagtc gcgcaattca
     5821 ataccgccct tgaccgccgt ccagaaaacg gagatgggtg ggccatgctc ggcagcgtct
     5881 acaaacaaca ggcaaaatac gatgctgcgg ccaacgcgct gcgtaaagcc attgcgcttc
     5941 tgcccaatca gccggggccc cacatcaccc ttgccggcgt tttgcaggag cagggcaaac
     6001 gggaagaagc ggcagtcgaa cgcaaaatcg ccgctgacct gacacgcgtt gccgtcaacc
     6061 ggcagcgtgc tgtttttgcc acaaacacag gaaatgcgct tttgcagaaa gaccagatcg
     6121 ccgatgctgt cgcacggtat caggaagcca tcagcagcga cccggcctat gccgaagcac
     6181 accgtcagct tgccgctgcc tatgaacgcc aggggcggtc ggctgatgcc gccagcgagc
     6241 gccagaaagc cgcgtcgctc gatttacaga aacagtagcc cgtcagctta taaagccgat
     6301 aaaatttctc cgtccatgaa ccgccgcaag tttcttcaat ctctcagccg caccgcgctg
     6361 gtcttgccgt tttctgatgt gctgtttatg gccacgccgc cctggaagcg cgctgcattc
     6421 tttgccgcac aggacgcgtc acagaacaag cctggcgcgc aacccaccta tgaagccaaa
     6481 ccaatgccac ctcctccagg cccgaagtct cccattgccg gaacgccgct cggtttgcag
     6541 ttcatagacg tggcaacaca atctgggctg aatgcaaaaa cgatttacgg aggcgaaggt
     6601 aaaaacaagt atctgttgga gacgactgga tgtggggttg ccttctacga ctacgacaat
     6661 gatggctggc ttgacatctt cttggtcaac ggatggcggc tggaaggctt ccctaaagga
     6721 caggaaccta cgtgccatct ctttaagaac aaccgcgacg gcacgtttac tgacgttacg
     6781 acaaaggctg gactcgcccg cagcggatgg ggccagggct gctgcgttgg cgactatgac
     6841 aacgacgggc acgatgacct cttcgtaagc tattacggac agaacgcgct ctttcataac
     6901 aacggagatg gcacctttac tgatgtcaca gcgaaagcag gcctgctgca agcgaaaaca
     6961 cgctggaact cgggctgcgc tttcctcgac tacgaccgcg atgggcacct cgatctattt
     7021 gtcgcaaact atattgattt cgatttgaag accgcaccta cgcctgagtc cggcccatgc
     7081 gaatataagg ggattctcgt ggcctgcggc cctcccgggc ttcccggcgg gaaaaatatt
     7141 ctctatcaca ataatggaga cggcacattc actgacgttt cagaaaaagc cggcatgtgg
     7201 aacactattg gcaattacgc cctcagcgtg gccgtcgccg acctcaacaa tgacggctgg
     7261 ccggacatct atgttgccaa cgactccact gcggccacgc tttatcagaa ccagaaagac
     7321 ggcacattca aggacatcgc tattgaagcc ggcgcggcct tttctcctga tggcaagccg
     7381 caggctggaa tgggcgtttc catcggcgat tatgaccgcg atggcaacct cgacatcgtt
     7441 aagacaaatt tcgccggaga cactgactcg ctttacacaa atcttggtga cggtacattt
     7501 gaggatcaca cctatctttc cggactcggc gtcaacacgc gctatctcgg atggggtgtc
     7561 ggcttcttcg acatggacaa cgatggctgg cttgatctct ttatgtcgaa tggccatgtc
     7621 tatcctgaag tcagcagttc aaaaacagag gccccttacg ccgaacataa atatctttac
     7681 cgcaatctgc ggaatgggcg ttttgaggag gtcacagaca aagccggccc gggcatcacc
     7741 acgcccgtag cagcccgcgg gtgtgctttt ggcgattacg ataatgatgg cgatctcgac
     7801 attgttgtga actgtgtcaa ttcccttccg cagcttcttc gctgcgactc ggagtggaag
     7861 cgtaactgga tcaaggtcaa aaccatcggc accaaatcca atcgcaccgg cattggagct
     7921 cgcgtgaaag ttgttgccaa atgccagcca gattcgcaac agccattgtt acaaatagat
     7981 gaaatacgta gtggtggcag ttactattca cagaatgacc tgcgtctgca tttcggacta
     8041 gaccaggcaa aaatggtaga tttgcttgaa atccgctggc cttccgggca ggtagacgcc
     8101 ttcaggaata ttgaggccaa caagctttac atcatcgaag aaggcagtaa aactccgaag
     8161 gccatatcac tcactgggct gaagaagtcc tgagttcggc tgtttttgcg catggaacca
     8221 cactctcatg gcatccgact gggattacgc acacacgcgc agtaagcacc accaatgcca
     8281 accagagcac atctgcgccc agcaaatgca caatctgcat ccaggtgggc gcaagcagcg
     8341 caaggtcggc aaatcccaaa aaatattgtg atgcgagcag cgcaaccact cccatcgcca
     8401 gcccacggtt tgcgcctttc catgcgcttt tgaaaatcag ccagcagatg aacgcaccag
     8461 caatgaaggc catcgtcgga tg
//
LOCUS       DASRBV010000006         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_557778, whole genome shotgun sequence.
ACCESSION   DASRBV010000006 DASRBV010000000
VERSION     DASRBV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557778"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFA02_00120"
     CDS             complement(<1..206)
                     /locus_tag="VFA02_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08278.1"
                     /translation="MHVFPLRSALSPQERQLRQRLILRDALALSSLFAIAIVLFFITL
                     ALFHSFSVHRERLKERWLARGERA"
     gene            278..1264
                     /locus_tag="VFA02_00125"
     CDS             278..1264
                     /locus_tag="VFA02_00125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU08279.1"
                     /translation="MQRREFLKSAALTALASTLTHEAHGAASGMPYRTLGRTGEKVSL
                     VGLGGHHIGRKYVSQEEGIRIIRTGLDSGVNFLDNCWDYNDGESERRMGLALKNGYRQ
                     KAFLMTKLDGRTVAAAQQQLEQSLQRLQTDHIDLIQIHEVIRMDDPERVFAPGGSLEY
                     LEKARKQGKVRYIGFTGHKSPEIHLHMIETAAKHNYTFDTVQMPLNPLDAHFDSFSAK
                     VIPAARKHNMGILGMKPLGDGLVLKSKTVTPVECLRFAMHLPVSVVITGCDSMQILQQ
                     ALDTAKNFHGMDETELNSILQKTATVAQNGQFELYKTSHNFDGTYHNPQWLG"
     gene            1252..2100
                     /gene="larE"
                     /locus_tag="VFA02_00130"
     CDS             1252..2100
                     /gene="larE"
                     /locus_tag="VFA02_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZU08280.1"
                     /translation="MAGLMELAEKHERLESRLRELGSVIVAYSGGVDSAYLAYAAHKI
                     LGENMLAVIADSPSLPRSHYRDAIAFAEEQCIPLRTITTHELEREEYAVNQADRCFHC
                     KDELFSVLEAERVKLGFAAVAYGLNTDDKKDFRPGQRAALKHSVAAPLAEAGLTKSDV
                     RALAHEAGLSVWDRPASACLSSRIEYGRRVTPETLRMVEDAEESLRALGLRNFRVRHH
                     GNIARIEIAVDEMEAVLAPETFRRMGAAVRTAGFDYVAVDCDGYRSGSMNEILPVEAL
                     AKAGGQ"
     gene            2214..>2785
                     /gene="larC"
                     /locus_tag="VFA02_00135"
     CDS             2214..>2785
                     /gene="larC"
                     /locus_tag="VFA02_00135"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HZU08281.1"
                     /translation="MRIGYLECFAGISGDMLLGALVDAGVSRALLEEAAQALNIGATL
                     KFSSVDRSGITAAKVDVLEAASGKIAEQHHHHHHEHEHHHEHSSEELPSRPGSRNDAM
                     EGHAVHDHTHHRHNHGHHHHSHVHGRTWNQIRELIHHAPLALETRELSLRAFEYLAQA
                     EAKIHGVPVEDVHFHEVGAIDTITDIVCAAA"
BASE COUNT          692 a          772 c          767 g          554 t
ORIGIN      
        1 aatgccctct cgcctcgcgc cagccagcgc tctttgagtc tctcacgatg cacagaaaat
       61 gagtgaaaca gcgccagcgt aataaaaaac aggacgatcg cgatagcaaa gagactggaa
      121 agggccagcg cgtcccgcag aatgagccgc tggcgcaatt gccgctcctg gggagaaagc
      181 gccgaccgta gggggaatac gtgcatcttt attcaggtta acctgcccat caatcaggca
      241 tctattcaaa ggagtaacat tatcggaagg agtatccatg caaaggcgcg aattcctcaa
      301 atccgcagct ctgaccgcac tggcctccac acttacgcat gaggcccatg gcgccgcatc
      361 gggaatgcct taccgcacac taggacgcac aggcgaaaag gtctcgcttg tcggtctggg
      421 cgggcatcac attggccgca agtatgtttc gcaggaagaa ggcatccgca tcattcgcac
      481 cggactcgat tctggcgtca atttcctgga caattgctgg gactacaatg acggcgaaag
      541 cgagcgccgt atgggtctgg ctttgaaaaa tggctaccgc caaaaagctt ttttaatgac
      601 caaactcgac gggcgcactg ttgccgccgc gcagcagcag cttgaacaat ccctgcaacg
      661 tcttcagacc gaccacattg accttatcca gatacacgaa gtcatccgca tggacgaccc
      721 ggagcgcgtc ttcgctcccg gtggatcact tgaatatctg gagaaggcgc gcaagcaagg
      781 caaggtgcgc tatatcgggt tcaccggaca taaaagcccg gagatacacc tgcacatgat
      841 tgaaaccgca gcaaagcaca actatacgtt tgataccgta cagatgccgc tcaatccact
      901 cgacgcgcac tttgacagct ttagcgcgaa ggtcattcct gcggccagaa agcacaacat
      961 gggcattctg ggaatgaagc cattaggcga tggccttgtg ctcaagagca agaccgttac
     1021 acctgtggaa tgcctgcgat ttgcgatgca cctgccggta agcgttgtca ttaccggatg
     1081 cgactccatg cagatattgc agcaggcgct cgatacagca aagaactttc acggcatgga
     1141 tgagaccgag ctgaacagca ttttgcaaaa gacggcaacc gttgctcaga atggacagtt
     1201 cgaactgtat aagacgtctc ataatttcga tgggacctac cacaatccgc aatggctggg
     1261 ctgatggaac tcgcagaaaa acacgagcgg ctggaatcac gcctgcgcga gctgggcagc
     1321 gtcatcgtgg cctattccgg cggagtggat tcggcctacc tcgcctatgc cgcgcacaaa
     1381 attctgggcg aaaacatgct cgccgtgatc gccgattcgc ccagcctgcc gcgcagccat
     1441 taccgtgacg caattgcctt tgctgaagaa caatgcatcc ctttgcggac gatcacgacg
     1501 catgaacttg aacgcgaaga gtacgcagta aatcaggctg accgctgctt ccattgcaag
     1561 gacgagcttt tctctgtgct cgaagccgag cgcgtcaaac tgggatttgc tgctgttgct
     1621 tacggattga acacggacga caaaaaagat ttccgccctg gacagcgcgc cgcgctgaag
     1681 cattcggtgg ccgcacccct ggcggaagcc ggactgacga agagcgacgt tcgcgcactg
     1741 gcgcatgaag ctgggctgag tgtgtgggac agacccgctt cggcatgtct ttcttctcgt
     1801 attgagtatg gacggcgtgt gactccggaa acattgcgca tggtcgaaga cgcggaagag
     1861 tctctgcgcg ctttggggtt gcgcaacttt cgcgtgcggc accacgggaa catcgcgcgc
     1921 attgagatcg cagtagacga aatggaagcg gtgttggccc cggagacatt tcgcagaatg
     1981 ggtgccgccg tgcgtaccgc cggtttcgat tatgttgcgg tagattgcga cgggtaccgc
     2041 tccggctcga tgaatgagat cctgccagta gaggcgctgg caaaggctgg cgggcaataa
     2101 aggatgtcaa tttcgaaaaa atgcaggcgt ttaaggcgct gacctgtttt tagctgcgca
     2161 gcgaagcact gacttgctga cgcgaagcgc tgacttgcct gacaataaga accatgcgaa
     2221 ttggatatct tgaatgcttt gccggaatca gcggcgacat gctcctcggc gccctcgtgg
     2281 acgccggtgt ttcccgcgcg ctgcttgaag aggccgcgca ggcgctgaat atcggcgcaa
     2341 cactcaaatt ctcttccgta gaccgcagcg gcattacggc ggcaaaagta gatgtgctcg
     2401 aagctgcaag cggtaagata gcagaacaac accatcacca tcatcacgaa cacgagcacc
     2461 accatgagca ttcctctgaa gaattgccat cccgaccggg atcacgaaac gacgcaatgg
     2521 aaggacatgc agttcacgac cacactcacc acagacataa tcacggtcat caccaccatt
     2581 cccacgttca cggacgtacc tggaatcaga ttcgtgaact gattcaccat gctccgctcg
     2641 cgctggaaac ccgcgaacta tcattacgag catttgagta tctggcgcag gccgaagcga
     2701 agatccatgg tgttccggtt gaagatgtcc atttccacga agtgggggcc atagacacaa
     2761 ttacggacat cgtctgtgcg gccgc
//
LOCUS       DASRBV010000007         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_51073,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000007 DASRBV010000000
VERSION     DASRBV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51073"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VFA02_00140"
     CDS             <1..1222
                     /locus_tag="VFA02_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HZU08282.1"
                     /translation="NCFPNTLDTTVEPGRFEGKPDTAVITGDIPAMWLRDSSAQVWPY
                     LPLAAHDGRLRELMEGVIRRQARCVLIDPYANAFMADLNAPPLKWSLGDKTALRRGVG
                     ERKWELDSLCYPIRLSYGYWKYTGDTRPFDATWKQAMALAIQTMTVQQRKHGNGPYHF
                     QRKSFVSTETLPDDGYGNPVNPVGLIASGFRPSDDACIFPFLVPSNLFAVTSLRQLAE
                     MSNTILHEASLANQAHSLAAEVESALRQYAIAQTSEGPIWAYEVDGFGSRLLMDDANV
                     PSLLGLPYLESSPDAALYQRTRRFIWSSHNPWFFSGTAGEGIGGPHEGQNMIWPMSQI
                     IYALTSNLDEEIRRMLAMLKTSSAGTGFIHESYDKDDPKKFTRAWFAWANSLFGELIE
                     RCATQKPELLRLTQ"
     gene            complement(1335..>5018)
                     /locus_tag="VFA02_00145"
     CDS             complement(1335..>5018)
                     /locus_tag="VFA02_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZU08283.1"
                     /translation="AIYPRLLEIARAQRSTIIFVNNRRIAERIAAAMNDLAGETIARA
                     HHGSLAAARRQEIEEQLKAGNIRALVATSTLELGIDMGAVDMVVQVEAPPSVASGMQR
                     IGRAGHRVGEASEGIIIPKYRSDLVACAAVTRAMHEGLVEATRYLRNPLDVLAQQIVA
                     VVAGLAVRAGMPLANPMQPGIFVDDLFALVRGAAPFATLSRAAFEGVLDLLAGRYPSD
                     EFAELRPRITWDRERNILTPREGAKSLAILNAGTIPDRGLYGVFLSKDEGRPVRVGEL
                     DEEMVFESRIGDTFVLGASTWRIDEIRQDRVLVSPAPGEPGRTPFWHGDRAGRPLEFG
                     KRIGRLVRELREMPRNTAMGRLVREHDLDPNAAENLLRFVADQEAATKLVPDDKTIVV
                     ERVRDEVGNWRVCVLTPFGARVHAPWAMAAAGKAGNAELMWSDDGFVLRFPDTEQPPD
                     AQVIALDASEASELVLQQLGSTAMFAAKFRENAARALLLPRRRAQGRSPLWQQRKRAH
                     GLLTVASQYPSFPIIVETYRECMRDVFDIPALEEILRSVEAGEVRVHVADTERPSPFA
                     SSLIFSYVMNFIYDGDAPLAERRAQALAINQEQLRDLLGDADLRELLDADAIAEVEEQ
                     LQLLVENYRVRSADGMHDLLLRLGDLSREEIARRLTSAELLPALDDLARTKRVVQIEI
                     AGEKRFIAVEDAARYREALGTMLPKGLPKQLLEPTSLSTPIRKNRVLEIVRRYARTHG
                     PFTLEEVTARFGLEAKQAEAALRELVADGRVVEGGFRPGGLHREWCDAEALRLIRRKT
                     LARLRKEVEPVEQPMLARFETHWQGVLQRRRGLDALLDTVEMLQGVPLPASLLEAQIF
                     PARTAKYSPADLDTLIAAGEIVWCGVDSLGERDGRIALYLADRMEKMLPPVAAETGPL
                     GPREEAILSELKRRGALFFAELHERVGGGYPGETLNALWALVWRGLVTNDTFHALRAY
                     TAKPVEKRAAKRVHNEQAFRSRRTTPPSAQGRWGLVPGASGSTTEWSHAIAQQLLQRY
                     GIVSRETVTQEGLPGGFSAVYDVFKAMEEGGRIRRGYFVTGLGGAQFALPAAVDLLRS
                     LRSRAPEKAEMVALAATDPANPYGAVMPWPENAVQSFLGRSVGATVILRNGELAAYLR
                     RNNPNLLIFLSGDEPSRDLAAFLFEMAQDELQKEGEGRRRGGLLISAINEQPAYRHPF
                     SRFLQQAGFSVAPAGFYVRRVMQ"
BASE COUNT         1036 a         1801 c         1240 g          941 t
ORIGIN      
        1 aaactgtttt cccaacacgc tcgacaccac cgtcgagcct gggcgctttg agggcaaacc
       61 cgataccgcc gtcatcaccg gagatatccc tgccatgtgg ctgcgcgatt cctccgcgca
      121 ggtctggcct tatctgccgc tcgccgcaca tgacggacgg ttgcgcgaat tgatggaggg
      181 cgtcattcgc cggcaggccc gctgtgtcct gattgatccc tacgccaacg cctttatggc
      241 cgacctgaat gcgcccccgc tcaagtggag cctcggagac aagaccgccc tcaggcgtgg
      301 agtaggcgag cgcaaatggg aactcgattc actctgctat cccatccggc tctcttatgg
      361 ctattggaaa tacaccggag acacacgccc ttttgacgcc acgtggaaac aggcgatggc
      421 gctcgccatt cagaccatga ctgtgcagca acgcaaacat ggcaatggac cataccactt
      481 ccagcgcaag tcctttgttt ctaccgagac actgcctgac gacggttacg gcaatccggt
      541 gaaccccgtc ggactcatcg cctccggctt tcgcccgtcc gatgatgcct gcatcttccc
      601 gttccttgtg ccttccaatc tgttcgctgt tacatctctg cgccagctcg cagaaatgtc
      661 gaacaccatc ctgcatgaag catcacttgc caaccaggcc cactcacttg cagcagaagt
      721 ggaatccgcg ctccgtcagt acgccatcgc gcagacgagc gaagggccca tttgggccta
      781 cgaagtggat ggcttcggca gccggctcct gatggacgat gcgaacgtcc ccagtctgct
      841 cggcctgcct tatcttgaaa gttctcccga cgccgctctt taccagcgca ctcgccgttt
      901 catctggagc agccacaacc cctggttctt ttcaggaaca gcaggtgagg gaattggagg
      961 cccgcacgaa ggccagaaca tgatctggcc catgtcacag atcatttatg cgctcaccag
     1021 caaccttgat gaagaaatcc gcagaatgct ggccatgctc aaaacctcgt ccgccggaac
     1081 ggggttcatc cacgaaagct acgacaaaga cgacccaaag aaattcaccc gcgcatggtt
     1141 cgcctgggcc aactcgctct tcggagaact gatagagcgg tgcgcgaccc aaaaacctga
     1201 gctgctgcga ctgacccaat aacgttgtca tcccggggag aagcgcaacg aaggctctgc
     1261 gttttcactg gttgtcacaa gtcacaacta ccggctgccc cgccctttgc gcagcaaaga
     1321 gcggagaccg ccacttactg catcactctc cggacataga accctgcggg ggccacagaa
     1381 aacccggcct gctgtaaaaa tcgagaaaat ggatgccggt acgcaggctg ttcattgatc
     1441 gccgaaatca gcaggccgcc ccgtcttcgt ccttcgcctt ctttctgcaa ctcgtcctgc
     1501 gccatctcaa acagaaatgc cgccaggtcc cgcgacggtt catcccccga caaaaaaatc
     1561 agcagattcg ggttgtttct tctcagatat gccgccagct cgccattgcg caaaatgact
     1621 gtcgccccca cgctgcggcc cagaaatgat tgcacagcat tctcgggcca cggcatcact
     1681 gcaccataag gattcgccgg gtccgtcgcc gccagcgcca ccatctctgc tttttccgga
     1741 gcacgcgacc gcagtgacct cagcaaatcc accgctgctg gcagggcgaa ctgcgccccg
     1801 ccgagtccag tcacaaagta tccgcgccgg atgcggccgc cctcctccat cgccttgaac
     1861 acgtcataca cggcactgaa tccgcccggc agcccctcct gtgtcactgt ttctcgcgac
     1921 acaatgccat atcgctgtag cagttgctgc gcgatagcat gactccactc cgtcgtcgat
     1981 ccacttgcgc ccggcaccaa tccccaccgc ccctgcgcgc tcggcggagt cgttctgcgc
     2041 gaacgaaacg cctgctcatt gtggacacgc ttcgccgccc gcttctccac cggcttcgca
     2101 gtataggcac gtagcgcgtg aaaggtatcg ttcgtcacca gcccgcgcca caccagtgcc
     2161 cacagcgcat ttaaagtctc tcccggatac ccgccgccca cgcgctcatg taattcggca
     2221 aagaacaatg ctcccctgcg cttcagctca gaaagaatgg cctcttcccg cggacccagt
     2281 ggccccgtct ccgccgccac aggaggcagc atcttctcca tcctgtccgc cagatacaac
     2341 gcaatgcgcc catcgcgctc gccgagcgaa tccacgccac accagacaat ctcacccgct
     2401 gcaatcagcg tgtctaaatc ggcaggagag tacttcgctg tccgcgccgg gaaaatctgc
     2461 gcttccagca gagatgcagg aagcggcaca ccctgcaaca tctccacggt atcgagcagc
     2521 gcatccagcc cgcgcctcct ctgcaggacc ccctgccagt gcgtttcaaa ccgtgcgagc
     2581 atcggctgct ccaccggctc gacttccttg cgcagccgcg ccaaagtttt tcggcgaatc
     2641 agccgcagtg cttcagcatc acaccactcc cggtgcagtc ctcctggacg aaacccgccc
     2701 tccaccacac gtccatcggc caccagctcg cgcaatgctg cttccgcctg cttcgcctcc
     2761 agtccaaagc gtgccgtcac ctcttccagc gtgaacggcc catgcgtccg cgcataccgc
     2821 ctgactatct ccaacacgcg atttttgcgt atcggggtag aaagagatgt gggttccagc
     2881 aactgcttcg gcaatccctt cggcaacatg gtccccagcg cctcccggta ccttgccgca
     2941 tcctcgacag caatgaagcg cttctctccg gcaatctcaa tctgcaccac acgcttcgtc
     3001 cgcgccagat catcgagcgc cggaagcaac tcggccgaag tcagcctccg cgcaatctct
     3061 tcgcgcgaca ggtcccccag ccgcagcagc aaatcatgca tcccatccgc cgaccgcacg
     3121 cggtaattct cgaccagcaa ttgcagttgc tcttccactt ccgcaatcgc gtccgcgtcc
     3181 agaagctcgc gcaaatccgc atcgcccagc aggtcgcgca gttgttcctg attgatggcc
     3241 agcgcctgcg cccttcgctc tgccagcgga gcatcgccgt catagatgaa attcatcaca
     3301 taactgaaga tcagcgacga cgcaaacggc gacggcctct ccgtgtccgc cacatgcacg
     3361 cgcacttccc ccgcctccac actgcgcaaa atctcttcga gtgcgggaat atcaaacaca
     3421 tcgcgcatgc actcgcgata cgtttccaca atgatgggaa acgaagggta ctgcgacgcc
     3481 actgtcagca gtccatgtgc gcgcttcctc tgctgccaca gtggagagcg cccctgcgcc
     3541 ctgcgtctcg gcagcagcaa agcgcgcgct gcattctcgc ggaacttcgc cgcaaacatc
     3601 gccgtcgagc caagctgctg caacaccagt tccgatgctt ctgaagcatc gagcgcaatc
     3661 acttgagcat cgggaggttg ctccgtgtcc ggaaagcgca gcacaaagcc atcatcggac
     3721 cacatgagct ccgcattgcc ggcttttcca gcagccgcca tcgcccacgg agcgtggaca
     3781 cgcgctccaa acggggtcag cacacacacg cgccagttgc ccacctcatc acgcacgcgc
     3841 tcgacaacaa tagtcttgtc gtccgggacc agctttgtcg ctgcctcctg atcagccaca
     3901 aatcgcagca ggttctccgc cgcattcggg tccagatcgt gctcgcgcac cagccggccc
     3961 atcgctgtat tgcgcggcat ctcgcgcagc tcgcgcacca gcctgccaat gcgctttcca
     4021 aactccagcg gacgccccgc cctgtcccca tgccagaacg gcgtcctccc cggctcgccc
     4081 ggagcaggtg acaccagtac cctgtcctgc cgaatttcat caatccgcca cgtggaagcg
     4141 cccagcacaa acgtgtctcc aatgcggctc tcaaacacca tctcttcatc cagctcgccc
     4201 acacgcaccg gcctgccttc gtcttttgag agaaacacgc cgtacagccc gcggtcggga
     4261 atcgtccccg cattcagaat cgccagactc ttcgcgccct cccggggcgt caggatattg
     4321 cgttcgcggt cccaggtaat gcgcggccgc aactcagcaa attcatctga cggatatcgc
     4381 cccgcaagca aatcgagcac gccctcaaat gccgcgcggc tgagcgtggc aaacggcgcc
     4441 gccccacgca ccaaagcaaa aagatcatcc acaaaaatcc ccggttgcat gggattcgcc
     4501 aaaggcatac ccgcccgcac ggccagtccc gccaccaccg ccacaatctg ctgtgccaga
     4561 acatccagtg gattccgcag ataccgtgtc gcctccacca gcccctcgtg catggcccga
     4621 gtcactgccg cgcatgccac caggtccgag cggtatttcg gaatgatgat gccctcgctt
     4681 gcctcaccaa cacggtgccc tgcgcgcccg atgcgctgca tcccgcttgc tacagaagga
     4741 ggggcctcca cctgcaccac catgtccacc gcgcccatat caatgcccag ttcgagcgtt
     4801 gaagtggcca ccagtgcccg gatgttcccc gccttcagtt gttcttcaat ttcctgccgc
     4861 cttgccgcag ctaatgaacc gtggtgcgct cgcgcaatcg tctcgcccgc cagatcattc
     4921 atggcggcgg caatgcgctc tgcaatacgc cggttgttta caaagatgat ggtggagcgc
     4981 tgcgcccgcg caatctcaag cagtcgcgga taaatcgc
//
LOCUS       DASRBV010000008         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_710059, whole genome shotgun sequence.
ACCESSION   DASRBV010000008 DASRBV010000000
VERSION     DASRBV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710059"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1352
                     /locus_tag="VFA02_00150"
     CDS             222..1352
                     /locus_tag="VFA02_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZU08284.1"
                     /translation="MFLLFLAVSAQAQRARDLGVPFEGTPGPLNAITDVRGVEVGNTT
                     LISGNGPLKVGIGPVRTGVTVIFPRGKANPDPVYAGWFSQNGNGEMTGTTWVEESGFL
                     EGPVAITNTHSVGVVRDAVIAWGLKHGTFHQDWALPVVAETWDGWLNDLDGFHVKPDD
                     VFHALEDAHTGAVPEGAVGGGTGMICYGFKGGIGTASRVLSKDEGGYTVGVLVQCNCG
                     ARRQLVIAGVPVGKEIPEDTPDAMFRDSAGTEKQGELGSIIIIVATDAPLIPTQLKRL
                     ARRATMGLARTGSISGNGSGDIFLAFSTANPHANDAPGPNTVKTVANERINPLFAATV
                     QATEEAIVNAMVGAKTMTGIDGHTVIGLPHEKLQQVLKKYGR"
     gene            complement(1403..2158)
                     /locus_tag="VFA02_00155"
     CDS             complement(1403..2158)
                     /locus_tag="VFA02_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZU08285.1"
                     /translation="MTQQTWNASQYARNARFVADLAGGVFELLDAKPGERILDLGCGD
                     GALTARMAATGADVTGVDSSPDMIAAAQSLGLNARVLNAEALDFDREFDAVFSNAALH
                     WVRNQDAMLSGVHRALKPSGRFVAEMGGHGNIAAIQVALAAVFTKYGIDTALDGQNYC
                     FPTPASYAARLRRHGFDVHYIELIPRPTPLPESGMRGWLETFCSALFARLSTSQRETA
                     LEETVALLRPVLCDERGQWTADYVRLRFLAIAQ"
     gene            complement(2188..2910)
                     /gene="bshB1"
                     /locus_tag="VFA02_00160"
     CDS             complement(2188..2910)
                     /gene="bshB1"
                     /locus_tag="VFA02_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HZU08286.1"
                     /translation="MPENSIADILAVAAHRDDVEQTCGGTLLKMKALGWRTAILDLTQ
                     GEAGTRGSAEDRAAEAAEAAKILGVSWRHALDIPDGRVENTYENRVKIVRVLREVRPR
                     VVILPYWTGRHPDHYTTSTLGYEACFLSGLAKLETGAPPHRPFKILYASLYADVRPSF
                     VVDITPYIEQRHEALMAYKSQYQNQQAGSGLFVPEEEVRERTFAEARHYGLLGGVRYA
                     EPFVQKEVGLVEDVMQIAVQSM"
     gene            3027..3890
                     /gene="bla"
                     /locus_tag="VFA02_00165"
     CDS             3027..3890
                     /gene="bla"
                     /locus_tag="VFA02_00165"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HZU08287.1"
                     /translation="MILLFMGTLLAPPPSHLYAQTNPNWTTPFPPHHIIGNVYYVGSR
                     DLAAFLITTPQGHILINSNLESSPDQIKKSVEQLGFHFSDIKILLISHAHFDHCAGSA
                     LIRKMTGAKYMVMDGDVSDVESGGRSNFYYSRSKDMYYPPAKVDRVLHDGDEVRLGGV
                     VLVAHLTPGHTKGCTTWTLKVQDSGKTYDVVIIGSPNVNPGYKLVNNSKYPQIAQDYD
                     RTFRVLKSLHCDVFLGAHGSYYGMQEKFARLQKGDANPFIDPDGYRSYVAEREQAFYK
                     ELKKQQAGDGI"
     gene            complement(3947..5356)
                     /locus_tag="VFA02_00170"
     CDS             complement(3947..5356)
                     /locus_tag="VFA02_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08288.1"
                     /translation="MSVLTACGKNYYFAGRNLPPSGVLNRVLVAVQNPSALAQGELLF
                     DDAFYDIRHSYNNQTNTFSISGYSGKLPVSIQNMPEEQQGAVYSIGDGSLTIIDYSGE
                     KVKSTFSASNLGGISQSVFVSRDLNYAYAAAQSSHSLTVYDHGSPVSLNLPGVYRVSV
                     NPGNTVVLAFVQNTNNVYSLVHLNDSQRQAAVNNPNWNGAVDCEPQNLPTYCVFPVAT
                     NNGASFDRPVKAVFSADGSAAWVLNCGPECGGTTAGVTKIPITSSALNPSGVGASGIA
                     LVATQTIAVPGGATNGVFDGNTFYVAGQAVLTAGNTYTVAGQQVQGGSNLLTGYLSVI
                     NTLTGQVTGQYSISDGTHNKMILADDNTLWIGSTQCESGERYNLSQTTAPGTPFGCIT
                     MFNTANNTALIESFKGDGTGIAAVTGLHKVYTAEGGQVYIYNTSDGSARDNSNVTVTG
                     TAYDVAYMDAGSDGNNTTY"
     gene            5523..>5929
                     /locus_tag="VFA02_00175"
     CDS             5523..>5929
                     /locus_tag="VFA02_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU08289.1"
                     /translation="MTVQQFGGPEVLAIGEAPVPTPGPGEVLLRVAAAGINRADILQR
                     QGHYPPPPGASPILGLEVAGNVIESGPGGTRWKPGDAVCALLPGGGYAEYCIASAGCC
                     LPVPKSVPLAEAAALPEAVFTVWANLFAQPWLRA"
BASE COUNT         1287 a         1714 c         1662 g         1266 t
ORIGIN      
        1 tgggctttgg atcagcatgg ggatcgtgca gaagcatggc ggcgccatcc agatcggcaa
       61 cagcaccgat cccgaatatc ccggcgcccg cgtgtgggtc tatctgccgg cgcacacgat
      121 ggccacgtct ttttcccgtt ccacgccata cattggataa atcttccctt ttccttccgt
      181 attttctgct gtagctttcc tgcatgcgga tttgctctgt tttgtttctc ctgtttctgg
      241 ccgtctcagc gcaggcgcag cgggcgcgcg atctgggtgt gccgtttgaa gggacccccg
      301 gaccgctgaa tgccatcacc gacgtgcgcg gagttgaagt cgggaacacc acgctcatct
      361 ccggcaatgg gccgctcaaa gttggcatcg gcccggtgcg cacgggcgtg accgtcatct
      421 ttccgcgcgg aaaagcaaat cctgaccccg tatatgcagg atggttcagc cagaacggta
      481 acggcgaaat gacaggtaca acatgggtgg aagaatccgg ctttctggaa ggccctgtcg
      541 ccatcaccaa cactcacagc gtgggagtag tgcgcgatgc tgtgattgcc tggggcttga
      601 agcacggtac tttccaccag gactgggcat tacctgtagt tgctgaaaca tgggacggat
      661 ggctgaatga tctggatggc ttccatgtaa agcctgacga tgtttttcac gctctcgagg
      721 atgcacacac aggtgctgtt cctgagggcg cagtgggcgg tggcacgggc atgatctgct
      781 atggcttcaa aggcggcatc ggcacggcct cccgtgttct gtcaaaagat gagggcggat
      841 ataccgtggg cgttctggtg cagtgcaatt gcggcgcgcg ccggcaactg gtgatcgcgg
      901 gcgtccccgt agggaaagag attcctgaag acacgcctga cgccatgttc agagatagcg
      961 ccggcacaga aaaacaggga gagcttggct ccatcatcat cattgtcgcc accgacgccc
     1021 cgctgattcc cacccaactc aagcggctgg cgcgccgtgc caccatgggc ctggcgcgga
     1081 ccggatcaat ctcaggcaat ggctccggcg atatctttct cgccttctcg acggcgaacc
     1141 ctcatgcgaa tgatgcaccg gggccaaaca ccgtgaagac cgttgccaat gagcgcatca
     1201 atccgctttt cgcagccacc gtgcaggcaa cggaagaagc catcgtcaat gccatggttg
     1261 gcgcaaaaac catgaccggc atagatgggc acacggtgat cggccttccg catgaaaaat
     1321 tgcagcaggt tctgaagaaa tatggacgat agcgagcaat gggccacccc gtccttcctg
     1381 ctccgcaaaa caccggaccg tcctattgcg caatcgccaa aaacctcagg cggacgtaat
     1441 ccgccgtcca ctggccacgc tcatcacaaa gaacaggccg caacaaagcc acggtctcct
     1501 caagtgcggt ttcgcgctgt gatgtggaca agcgcgcaaa gagggcggag cagaaagttt
     1561 cgagccatcc tctcataccg ctctccggca gaggtgtggg acgcggaatc agctcaatat
     1621 agtgaacatc gaaaccgtgg cggcgcagac gggccgcgta gcttgccggc gtaggaaaac
     1681 aatagttttg cccatcgagc gcggtatcaa ttccgtattt tgtgaaaact gcggccagcg
     1741 ccacctggat cgccgcaata ttgccgtgac ctcccatttc tgcaacaaat cgcccgctcg
     1801 gtttcagcgc gcgatgcacg ccggacagca ttgcgtcctg attgcgtacc caatgtagtg
     1861 ctgcgtttga aaaaacagcg tcaaactccc ggtcgaaatc gagcgcttcg gcattgagca
     1921 cgcgcgcgtt cagtcccaaa gactgcgccg cggcaatcat gtccggcgag ctgtccacgc
     1981 cagtcacatc tgcgccggtt gcggccatcc tggctgtgag cgcgccatcg ccgcagccga
     2041 ggtccagaat gcgctcgccc ggcttcgcat ccagcagctc aaacacgccc ccggcaagat
     2101 cagcaacaaa acgcgcattg cgggcatatt gcgaggcatt ccaagtttgt tgggtcatgg
     2161 atttatttta gggtgattgg ccctggttca catggactgc accgcaatct gcatcacgtc
     2221 ttcaaccagg ccaacctcct tctgcacaaa tggttcggca tagcggacgc cgcccagcaa
     2281 cccgtaatgt cgcgcttcgg caaatgtccg ctcgcggacc tcttcttccg gaacaaagag
     2341 accgctgccc gcctgctgat tttgatattg cgacttgtag gccatcagcg cctcgtgccg
     2401 ctgctcgatg tatggagtaa tgtcaaccac aaagctcggg cggacgtcag cataaagact
     2461 cgcatagaga atcttgaatg gacggtgcgg gggcgcgccg gtctcaagtt tggcgagccc
     2521 tgaaagaaag cacgcctcat accccagggt ggatgtggtg tagtggtcgg gatggcggcc
     2581 tgtccaatac ggaagaatga cgacacgcgg acgcacttcg cgcaacacgc ggacgatctt
     2641 cacacggttt tcgtaagtgt tttccacgcg gccgtccggg atgtcgagcg catgccgcca
     2701 gcttacgccc aggattttcg ctgcttcggc tgcttcggct gcacgatctt cggccgagcc
     2761 gcgcgtgccc gcttcgccct gcgtcagatc aagaatggcc gtgcgccatc ccagcgcctt
     2821 catcttcaga agagtgccgc cacacgtctg ctctacgtcg tcgcggtgtg cggcaacggc
     2881 aaggatgtct gctatcgagt tttcaggcat gtccctaaga atactgaagc gccggaggta
     2941 tctgaaagat tacaggtaac agaaattcac agccacgcac agcccgtccc ggtgtcatgc
     3001 tgttgcctat gctttcccgc ctgtacgtga tcttactctt tatgggcact ctgcttgcgc
     3061 cgcccccaag tcatctctat gcgcaaacca acccgaattg gaccacgccc tttccgccgc
     3121 accatattat tggaaacgtg tattacgtcg gaagcaggga cctggctgct ttcctcatta
     3181 cgaccccgca gggacacatc ctgatcaata gcaatctgga aagctcgccc gatcaaatca
     3241 aaaagagtgt cgaacaactg ggctttcatt tcagcgacat caaaatactg ctcatcagcc
     3301 acgcacactt tgaccactgc gccggaagcg ccctcatcag gaagatgact ggagcgaaat
     3361 atatggtgat ggacggcgat gtttccgacg tagaaagtgg cggtcgaagc aatttttact
     3421 acagcaggtc aaaggacatg tattatccgc ctgcaaaggt ggaccgcgtt ctgcatgatg
     3481 gcgatgaagt gcgtctggga ggtgtggtgc tggtcgcaca tctcacccca ggacacacaa
     3541 aaggttgcac cacatggacc ctgaaggtac aggacagcgg caaaacatat gacgtggtga
     3601 ttatcggcag tcccaatgtg aatcctggat acaaactggt aaacaactcg aaatatccac
     3661 agattgcgca ggattatgat cgcacgtttc gcgtcctgaa gtcattgcac tgcgatgttt
     3721 ttctgggagc gcatggcagc tattacggaa tgcaggaaaa attcgcccgc ttgcagaaag
     3781 gtgacgcgaa tccgtttatt gaccccgatg gatataggag ctatgtggca gaacgtgaac
     3841 aggcattcta taaggaactg aagaagcaac aggcaggaga cggaatatag atctcagggc
     3901 acaggacctg cttttattga gcgattttgt ccctatgccc tgtaccctaa tacgttgtat
     3961 tattcccgtc gctgcccgca tccatgtagg cgacatcata ggcggtcccg gttacggtaa
     4021 cgttggagtt gtcgcgcgcc gatccatcgc tggtgttata gatatagacc tgtccgcctt
     4081 ctgccgtgta gaccttgtgc agtccggtga ccgcggcgat tccggtgccg tcgcccttga
     4141 aagattcgat aagcgcggta ttgttcgccg tattgaacat ggtgatgcag ccgaagggcg
     4201 ttcccggggc cgtggtctgg ctcaggttat agcgctcacc cgattcgcac tgcgttgagc
     4261 cgatccaaag cgtgttgtcg tcggccagaa tcatcttgtt gtgtgttcca tcgctgatgg
     4321 agtactggcc tgtcacctgt cccgtaaggg tgttgatcac cgacagatat cctgtaagca
     4381 gattgcttcc accctgcacc tgctgccctg ctacggtgta cgtgttccct gccgtgagca
     4441 cggcctgccc tgcaacgtag aaggtgtttc catcaaagac accattcgtt gcgcctccgg
     4501 gtactgcaat ggtctgcgtg gccaccagag cgatgccgct ggcgcctaca cccgagggat
     4561 tcagtgcaga gctggtaatg gggattttgg taacaccggc agttgtacca ccgcactccg
     4621 gaccgcagtt cagcacccat gccgccgaac cgtcggccga gaagaccgcc ttcaccggac
     4681 gatcaaagct ggctccgttg ttcgtggcca ccggaaagac acaatatgtc ggaaggttct
     4741 gcggctcaca gtccactgcc ccattccagt tcggattgtt gactgctgcc tgtctctgac
     4801 tgtcgtttaa atgaacaagg ctgtatacgt tgttcgtgtt ctgcacaaag gccagaacga
     4861 ccgtgttgcc ggggttcaca gagacacggt atacgccggg aaggttcagc gagaccggac
     4921 tgccgtgatc ataaaccgtc agcgaatgcg aggattgcgc cgctgcgtag gcgtaattca
     4981 ggtcgcggga gacgaagacg ctctgtgaaa tgcccccaag attactggca ctaaacgtgc
     5041 tcttgacctt ctcgccggag taatcaatga tggtcaggct gccgtcgccg atggagtaga
     5101 cggccccctg ctgctcttcc ggcatgttct gaatgctgac cggcagcttt cccgaatagc
     5161 cgctgatgga aaaggtgttt gtctggttgt tatagctgtg gcggatgtca taaaaggcat
     5221 cgtcaaagag caattcgccc tgcgctaacg cgctcgggtt ctggaccgcc accagaacgc
     5281 ggttcaatac tccgctggga gggagattgc ggcccgcgaa gtaataattc ttaccgcaag
     5341 cggtcaggac cgacacggcg cacaaaatgc cagcagcgaa aaacagactt cttttcttca
     5401 aaaccttgag acctcaatcc gtttctaaat acggaatttg caggaaaagc caagtataac
     5461 aaagcgaaca gacatctaac gaagcgactg tcgttttcca atattctgga tctatgcgca
     5521 tcgtgactgt tcagcaattc ggcggcccgg aagttctggc catcggtgaa gcccccgttc
     5581 ccacgccagg tccgggtgaa gtcctcctcc gggtcgcggc agcaggtatc aaccgggccg
     5641 acatcctcca gcggcaaggg cactacccgc ctccaccggg agcatcgcca attcttggac
     5701 tggaggtcgc aggaaacgtc atcgaatccg gcccaggcgg tacgcgatgg aaacccggcg
     5761 acgcggtctg cgcgcttctc cccggcggcg gttacgccga gtactgcatc gccagcgccg
     5821 gatgctgcct gcctgttccg aagtccgttc cgctggcaga ggccgctgcg ttgccggaag
     5881 ccgtctttac cgtctgggcc aatctgtttg cgcagccctg gctccgcgc
//
LOCUS       DASRBV010000009         6510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_304424, whole genome shotgun sequence.
ACCESSION   DASRBV010000009 DASRBV010000000
VERSION     DASRBV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304424"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFA02_00180"
     CDS             <1..700
                     /locus_tag="VFA02_00180"
                     /inference="COORDINATES: protein motif:HMM:NF045135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HZU08290.1"
                     /translation="VRDGSFTYKFDPDSLYSLTTTTGQGRGTAEPPPQKPFPFPYRED
                     FEKTSLHRAPKYLSDQDGAFEAVSCNGRAGRCLEQVITEKPIPWGPLPDPWTLAGDVN
                     WTDYQLDTDARLDQPGNITLIGRVDSADVFKDGKAHYPSAYVFVVNEKGEWKLLSCAY
                     KRPDRVLAQGAVGFAPSKWHHLRLRFAGKQITASIDEHSTASVEDADHTHGMFGIGTG
                     WNRAQFDNVSVVRP"
     gene            complement(964..2844)
                     /locus_tag="VFA02_00185"
     CDS             complement(964..2844)
                     /locus_tag="VFA02_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZU08291.1"
                     /translation="MADVLNPEQTESTPLNTELETPTLEAAAEHETPSLETTHNPEAT
                     SSEHAPGPAEHSVAVQQEDEPDLNSMEDFAAVLESFDREQAEQAAAQAFDDQHIVQGT
                     VIKLTDKHVVVDVGLKSEGLIPIEQVLDHTGQPKLQPGETVEVVIEREEPEGGYLLSY
                     EKAVRHRVWDTIERAYNEKTNLTGTVLSRVKGGLTVDLGIKAFLPGSQVEVRPVRNLD
                     AYIGQPIEVRIIKLNKKRGNVVVSRKEILEEEISARRAQTLEHLEEGSILTGTVKNLT
                     DYGAFVDLGGIDGLLHITDMSWGRLTHPRDLVNVGDEIQVKVLKFDREKQRVSLGFKQ
                     LTPDPWLDAAERYPIGARVRGRVLSVTDYGAFVELEQGIEGLVHVSEMTWSKRMKHPS
                     KIVKPGDEVETVILAVNPQDRRISLGMKQLLENPWEHLSERYPAGTVVEGRVRNLTDF
                     GAFIEIEDGIDGLVHVSNLSWTKRVKHPSEVLKKGEKVRAVVLGVEPENRRLSLGVKQ
                     LQPDVWETFFATHRIGDVVHGKVLRTAQFGAFVEIAEGIEGLCHVSEATDTNGIPVKL
                     EAGQEHDFKIIKMNPDEKKIGLSLRAVGEEASRADVEAYKQPVSSSTTTLGDLVNWKR
                     EQ"
     gene            complement(3682..4851)
                     /locus_tag="VFA02_00190"
     CDS             complement(3682..4851)
                     /locus_tag="VFA02_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZU08292.1"
                     /translation="MYALSLIAGIACIALALLDSFQTVILPRRATGRFRITHLFYAMT
                     WRPWAWLSARVRNERKRETMLSFYGPLSLVLLIIVWAGALVLGFALIFYGLGSPFTDP
                     LGRAVGLRSDLYVSGTTLFTLGLGDVVPHSYVIRDLVILEAGMGLGFVAMVIGYFPVL
                     YSAFSRREVSISLLDARAGSPPTAAELLRRHSFDGGDEALTLLLEEWERWSAELLESH
                     ISYPLLCYFRSQHTHQSWISALTAILDTCALLIAGVQERPARQAQLTFAMARHALADL
                     SQIFSLGPANNMPDRLPEQQFHALYDQLCETSMRLCRDPQSGERLTRLRQMYEGHAQT
                     LSNYLCMPLPPWAPEHKRRDNWLLVATIRAEAEAAGNPKASTSDSIVLDEEHHAF"
     gene            complement(4898..5881)
                     /locus_tag="VFA02_00195"
     CDS             complement(4898..5881)
                     /locus_tag="VFA02_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZU08293.1"
                     /translation="MTTASGGTLIYRKNMQLLRNGNSLPRVDEIRAELVRKVAAHARQ
                     EGENPTPVPWFRLYRRSAPLACTSTTYEPGLVIFAQGRKRVHIGETTYTCDESTFLLT
                     TIDLPVVSQIVEASAENPQLALLFQLDLSVVRKLLSEEEFRLTHSSSGTRGMAVGTTS
                     REMLDASLRLVNLLDSPRDIPFLSRLLQQEILYRLLHTPQGQNLRAIATLGEQSNRVA
                     KAVYWLRANYDQPLRIEHLAEIAQMGISTLHHHFRQMTGMSPLQYQKRLRLHVARERM
                     LHNGLDAARAAFEVGYESPSQFSREYSRFFGLPPMRDVKTRRLEFTGAAGN"
     gene            5996..>6510
                     /locus_tag="VFA02_00200"
     CDS             5996..>6510
                     /locus_tag="VFA02_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU08294.1"
                     /translation="MYKAKAYSAASETAPLVSTTISRREVTEHDVQIQILFCGICHSD
                     LHQVRNEWREVMPTVYPCVPGHEIVGRVVKTGPAVTKFKPGDLAAVGCMVDSDGTCPE
                     CEAGLEQFCPNMTLTYNSRDKHLGGVTYGGYSDSIVVNERFVLRVPENLDLAGAAPLL
                     CAGITTYSPMR"
BASE COUNT         1358 a         1764 c         1968 g         1420 t
ORIGIN      
        1 ggtcagggac gggtcattta cttataagtt tgatccggac tcgctgtatt ccctcaccac
       61 gacaacaggg cagggcaggg gcacggctga accgcctccg caaaaaccgt ttccatttcc
      121 ttatcgtgaa gatttcgaga agacgtcgct tcatcgcgct ccgaagtatt tatctgatca
      181 agacggagca ttcgaggccg tttcgtgcaa tggccgcgcg gggcgctgtc tggagcaggt
      241 catcacggag aaacctattc cctgggggcc gctgccggac ccgtggacgc ttgcaggcga
      301 tgtgaattgg accgactatc agcttgatac tgatgcccgt cttgaccagc cgggaaatat
      361 aaccctgatc gggcgcgtgg attccgcgga tgtcttcaag gatggcaagg cgcattatcc
      421 gagcgcatat gttttcgtgg tcaacgaaaa aggcgaatgg aagctgctct cctgtgctta
      481 taaaaggcct gatcgtgtgc tggcgcaggg agcagtaggt tttgctccgt caaagtggca
      541 ccatctccgg ctcaggtttg ccggaaagca gattacggcg agcatagatg agcacagcac
      601 tgccagcgtg gaagatgcag accacacgca cggcatgttc ggcattggca cgggctggaa
      661 ccgcgcgcaa ttcgacaatg tttccgtggt gcgtccgtga caggaaaatc ttcgacgcga
      721 gtataggcag cttgtctgtg tgacccgcag cgcaattgtt cgcctccatg cgatttggga
      781 gattcgccgg ccggcaatcg ttctgcattc atgtgtggag gccgtctgtt caaagaaata
      841 ccgggtggct gctctgatgc ggctgcaata atccggattg gcctctgttg atttgaatgt
      901 atatggagaa aagaaaaacg ccatccgcat gggatggcgt tgtttgtgtc ttttatgggt
      961 gaattactgt tcgcgtttcc agttcaccag gtcgcccagc gttgtggttg agctggagac
     1021 gggctgcttg taggcttcca catcggcgcg gctggcttct tcgcccacag cgcggaggct
     1081 taggccgatc ttcttctcat ccggattcat cttgatgatt ttgaagtcgt gctcctgtcc
     1141 ggcttcgagc tttaccggaa taccgttcgt gtctgtggcc tctgagacgt ggcagagtcc
     1201 ttcgatgccc tctgcaattt ccacaaaggc cccgaactgc gcggtgcgca gtaccttgcc
     1261 gtggaccaca tcgccgatgc ggtgcgtggc gaagaaggtc tcccatacgt cgggctgaag
     1321 ctgcttgacg cccagcgaga ggcggcggtt ctccggctct acgccgagca ccacggcacg
     1381 gaccttctca cccttcttca ggacttccga aggatgcttc acgcgcttgg tccagctgag
     1441 gttgctcacg tggacgaggc cgtcaatgcc gtcctcaatc tcgatgaacg cgccgaagtc
     1501 agtcagattg cgaacacggc cttcgacaac agtgcctgcc ggataacgct cggagaggtg
     1561 ctcccacgga ttttcaagga gctgcttcat gcccagcgag atgcggcggt cctgtggatt
     1621 gacggccagg attactgttt ctacctcgtc gcccggcttg acgatcttcg acggatgttt
     1681 catccgcttc gaccaggtca tctctgaaac gtggacaaga ccttcaatgc cctgctccag
     1741 ctcaacaaac gcgccgtagt cggtcacgct caggacgcgg ccacggacgc gggcgccgat
     1801 cgggtagcgc tcggcggcat ccagccatgg gtccggggtc aactgcttga atccgaggga
     1861 aacgcgctgt ttttcgcggt caaacttcag gacttttacc tggatctcat cgccgacgtt
     1921 caccagatcg cggggatgcg tcagacggcc ccaggacata tctgtaatgt ggagcaggcc
     1981 gtcaattccg cccaggtcaa caaacgcgcc gtagtcggtg aggttcttca ccgttccggt
     2041 gaggatgctg ccctcttcga ggtgctccag cgtctgtgcg cgccgggcag aaatctcctc
     2101 ttcgaggatc tctttgcggg aaaccaccac attgccgcgc ttcttgttca gcttgatgat
     2161 gcgcacttcg atcggctgcc cgatataggc gtccagattg cgcacggggc ggacttccac
     2221 ctgtgaaccc ggcaggaatg ccttgatgcc caggtcaact gtcaggccac ccttgacgcg
     2281 gctgaggacc gtaccggtaa ggttggtctt ttcgttgtag gcgcgctcaa tggtgtccca
     2341 cacgcggtgc cgcacggcct tctcatagct cagaaggtag ccgccttccg gctcctcgcg
     2401 ctcaatgacg acctcaacgg tctcaccggg ctggagcttc ggctggccgg tgtggtccag
     2461 gacctgttcg atgggaatga gaccttcgga cttcaggccg acatccacga ccacgtgctt
     2521 gtcggtcaac ttaatgaccg tgccctgcac gatgtgctgg tcgtcgaagg cctgtgccgc
     2581 ggcctgctcg gcctgctcgc ggtcaaagga ctcgagcacg gcggcaaagt cttccatgct
     2641 gttcaggtcc ggctcatctt cctgctggac ggcaacagag tgctcagccg gcccaggagc
     2701 gtgttcagag gaagtagctt ccgggttgtg ggtggtttcc agggacggcg tttcgtgctc
     2761 cgccgctgct tcaagcgttg gggtttcaag ttcggtgttc agaggagtgc tctcggtttg
     2821 ttcaggattt aagacgtctg ccataacagc cagctcagtg ggactcggtc ggcggccccg
     2881 gcctccgccc tgcaaattgc cttgcagttt atttcttcgc ttacctaaat agattcaggt
     2941 ggatgtagcg aagattcggg aatcgtctta ctctgcgagt cagaccgcgc ggaagaaccg
     3001 cagcgagaca gatgcgtgcc tgaaacactc acgtccatct cagttgggaa ctccgccagg
     3061 caggaacatg caggagcggc gattacagat ctgtgtcaga atcccgcgca cacagacccg
     3121 atgaaaacag gatagaactg tacgctggga gtgtcaaacg aacggcattc gttttcatgg
     3181 atttccaccg ctttcaagga aaagcgcaaa attttaccgg aagatttgtc atgcaactgc
     3241 gcttccaggc cacgaaaggg cacgaccatg cttcggatgg atcgagcatg ggctgggttt
     3301 tccacagcag cgtcctggtc tcaggtacaa gaccgcacag aagacggcct tcaccctctg
     3361 cattactcac acaatttcag gtagttatcg aatctgcctt tggcagtgag atgcaaacgc
     3421 aaaattcttt ttaaagatct ggtcaagcct tttagcgtgg gagtaatata ggccgcaaat
     3481 caataaaaat tcatttttat tcgatggagt aaagatgcta gtgattgttg ctatgatcgc
     3541 cggagtctat ctggcttgct tcctctggtt ccgctcgttg tgcaggtccg caatccggat
     3601 ccatcgcttt cctgtgcatc aggaaagggt ctcgaagctg atcccttttg aacgcggtct
     3661 ggaaatacgg tccagaagcc atcaaaacgc gtgatgctct tcatccagca caatggaatc
     3721 tgaagttgag gccttaggat tcccagcggc ctcagcctct gctctgattg ttgcaaccaa
     3781 cagccagttg tcgcgtcgct tgtgttcggg cgcccagggc ggaaggggca tgcacaggta
     3841 attgcttaat gtttgtgcat ggccttcata catctgacgc agcctggtca ggcgttctcc
     3901 cgattggggg tcgcggcaga ggcgcatgct ggtctcgcaa agctggtcgt agagtgcatg
     3961 gaactgctgc tcaggcaggc ggtcgggcat gttgtttgcc ggccccaggg agaagatttg
     4021 tgacaggtca gccagggcgt ggcgggccat ggcaaatgtg agttgcgcct ggcgggcggg
     4081 gcgctcctgc actccggcaa tgagaagggc gcaggtgtcc agaatggcgg tgagggcgct
     4141 gatccagctc tggtgcgtgt gctgcgagcg gaaatagcaa agcagtgggt aggagatatg
     4201 gctctcgagc agctccgccg accagcgttc ccattcctcg agcagcagcg tcaacgcctc
     4261 atcaccgccg tcaaacgagt gccgacgcag aagttctgcc gcagtcggag gcgacccggc
     4321 gcgcgcgtcg agcagagaga tactgacttc gcggcgggag aatgcgctgt agaggactgg
     4381 aaagtagcct atcaccatgg cgacgaagcc caggcccatc ccggcttcca gaatcaccag
     4441 gtcgcggatg acgtagctgt ggggcaccac gtcgccgagg cccagggtaa agagtgtggt
     4501 tccgctgacg tagaggtcgg agcgaaggcc gaccgcgcgt cccagcgggt cagtgaaggg
     4561 cgagccgagt ccgtaaaaaa tcagggcaaa gccaagcacc agcgctccgg cccagacaat
     4621 gataagcagg accagtgaaa gcggaccgta gaagctgagc atggtttcgc gcttgcgctc
     4681 gttgcggacg cgagctgaga gccaggccca gggcctccat gtcatggcat agaaaagatg
     4741 cgtaatgcgg aaccttcccg tggccctgcg cgggaggatg acggtctgga aagagtcgag
     4801 cagcgcaagc gcgatacagg cgattccggc aatcaggctg agggcgtaca ttgttttcgt
     4861 ctggtgggct taggagaaca ggagaaacaa aggcgagtca attgccagca gcaccagtaa
     4921 actccaatct tcgcgtcttc acgtcacgca tgggtggcag gccgaagaag cggctgtatt
     4981 cgcggctgaa ctggctgggg ctttcatagc caacttcaaa ggcggcgcgc gcggcgtcga
     5041 ggccgttgtg caacatgcgc tcgcgggcca cgtgcaggcg cagacgcttc tggtattgca
     5101 atggactcat cccagtcatc tgacggaaat ggtgatgcag agtagagatg cccatttgcg
     5161 ctatttcagc cagatgttca atgcgcagag gctggtcgta gtttgccctc aaccagtaga
     5221 cggcctttgc cacgcggtta ctctgctcgc cgagcgtggc gatggcgcgc agattctggc
     5281 cctgcggagt atgcaacaag cgatagagga tctcctgctg taaaagccgg ctgaggaaag
     5341 ggatgtccct gggcgagtcc agaagattga cgagccgcaa agaggcgtcc agcatttccc
     5401 gtgaagtggt acctacggcc atgccgcgtg tgccggaaga ggaatgggtc aggcgaaact
     5461 cctcttcact cagcaatttg cgcacaacgg acagatcaag ctggaacagg agtgccaact
     5521 gaggattttc tgcgctggcc tccacgatct ggctgacgac gggaaggtct atagtggtca
     5581 gcaaaaatgt tgattcatcg cacgtataag tcgtttcacc aatatgaacg cgtttcctgc
     5641 cctgggcaaa gatcacgagt cccggctcat aagttgtgga agtgcaggcc agaggtgcgc
     5701 tgcggcggta gagcctaaac cacggaacag gtgttggatt ctcgccttcc tgtctggcat
     5761 gcgcagcgac tttacgcacc agttcagccc gtatctcgtc tacccgcggg agagaatttc
     5821 cgttcctgag caattgcatg ttctttctat agatgagagt acctccggat gcggtcgtca
     5881 tgctcgcaaa atttccggcc agcgagagaa tcaggcaaga agtcggaaga atcaggatat
     5941 acccgggcgc tggctttcat ctctaatcac gtaaagccaa aaaaggagca tgactatgta
     6001 caaggcaaag gcctactcgg cggcgagtga gacagcaccg ctggtctcga cgacaatctc
     6061 aaggcgcgag gtgacggagc atgatgttca gatccagata cttttctgtg gcatctgcca
     6121 ctctgacttg catcaggtgc gtaacgaatg gagagaagtg atgccgacgg tctatccctg
     6181 cgttccggga catgagattg ttggacgcgt cgtaaagact ggtcctgcgg tgacgaagtt
     6241 caagccgggc gacctggccg ccgttggctg catggtggat tccgacggga cctgtccgga
     6301 gtgcgaggcc gggctggagc aattctgccc gaatatgacg ctgacttaca attcccggga
     6361 caagcacctg ggcggtgtga cctacggtgg ttattccgac agcattgttg tgaatgagcg
     6421 atttgtactg cgtgtgcctg agaaccttga tctggccggg gccgcgccgc tgctttgtgc
     6481 cggaatcacg acgtattcgc cgatgcgcca
//
LOCUS       DASRBV010000010        12032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_653,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000010 DASRBV010000000
VERSION     DASRBV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="thrC"
                     /locus_tag="VFA02_00205"
     CDS             <1..1263
                     /gene="thrC"
                     /locus_tag="VFA02_00205"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZU08295.1"
                     /translation="EVVYPSWSAGFAAAEEKKPNASALRWLWKERCSSNEEADQSGVW
                     RFRELLHILRDEKNIVSLREGNTPLYSLRRTARSTGIAKLYAKHQGMNPTGSFKDTGM
                     TAALSVAYELGYEWVACASTGNTSAAMAAYAARAGMRSLVLIPQGKIAWGKLSQAIDY
                     GALTIQLRTDFDGCVRVLAEIVRRAPVYLLNSVNPYRLEGQKTPALEIAEALEWEVPD
                     HVIVPGGNLANSSALGKAFLEMKHLGLTARLPKISVIQAEGANPLYRAMRENGGEALY
                     PVEAHTRASAIRIGNPASWKKAVRVIRETGGWCEQASEAEIALAKAEIGEEGIGCEPA
                     SAVTLAGLKKLVREGKVQSQETVVLLLTGHTLKDSDYTIAYHRGDLLRPDEVGALRTE
                     IEVTRRNAVEMDASADLVLRELERAKER"
     gene            1260..2177
                     /gene="thrB"
                     /locus_tag="VFA02_00210"
     CDS             1260..2177
                     /gene="thrB"
                     /locus_tag="VFA02_00210"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZU08296.1"
                     /translation="MSLRPIRLKLPATSANLGPGFDALALALSLYLEIEAAPGEKYSI
                     EASGRNVDICGALEKNLLLHTYKTILEQQGVQEVPLTLKVRNGIPVGMGLGSSAATRL
                     AGAALAAHFGHLGWDRSRILTEASRLEGHPDNTAACWLGGYTVACWHGDEVVAISLTP
                     RAYWRALVVIPQNPLSTTLSRKVVPETFHRSDAVANIQRVALLTAAFASGHSDLLSTA
                     VEDRMHQPYRAEVCPLLKKLLPLSGRENVLSVTLSGAGPSVLLLVESDFQINGVKDMV
                     RQHLEGEPVEILTCHIELDPVVFQQNDSN"
     gene            2421..2753
                     /gene="trxA"
                     /locus_tag="VFA02_00215"
     CDS             2421..2753
                     /gene="trxA"
                     /locus_tag="VFA02_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZU08297.1"
                     /translation="MAGLAVVEVNDTNFEQEVLQSDQPVLVDFWAAWCGPCKALAPIV
                     DEVAAEYSGKLKVMKMDVDRNQATPMRYGIRGIPALLLFKGGKVADQIVGYVPKDTIA
                     RSIARVIG"
     gene            complement(2808..3413)
                     /gene="lexA"
                     /locus_tag="VFA02_00220"
     CDS             complement(2808..3413)
                     /gene="lexA"
                     /locus_tag="VFA02_00220"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZU08298.1"
                     /translation="MAVTRRQKEVLDFISGFVQRNGYSPSFEEIARGLGLKSLATVHK
                     HITNLQSKGLLERAHNRSRSIDVLPPRSKSAVPDRLPLAGRIAAGFPVEAPETAESIS
                     LSDIIGNRDVFVLQVRGDSMRDEHIVDGDYVLVEKTRTAHQGEIVVALVNGAETTLKR
                     YYIEGPMVRLQPSNAEMEPIYVPADQVAIQGRALGVLRRYA"
     gene            3659..4336
                     /locus_tag="VFA02_00225"
     CDS             3659..4336
                     /locus_tag="VFA02_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU08299.1"
                     /translation="MRFLVADDDQALARFLRRGFEADGHQVMLAPDGQTAMEMFVSES
                     PDLTVLDLDLPRKDGIEVLRFLRSVSDEAPVLVLTARNEMETRVRCLEMGADDYVLKP
                     FSLRELRARCRSLMRRRQGAGVVLRYADIEVNRVERTVVRAGDAVQLTNKEFALLEFL
                     LLNRGRCVSRAELLQRVWEMSPEAGANVVDVYVNYLRRKLKNAGGPSLIQTVRGHGYS
                     VGMKTNS"
     gene            4360..5583
                     /locus_tag="VFA02_00230"
     CDS             4360..5583
                     /locus_tag="VFA02_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU08300.1"
                     /translation="MSTAALVKRSVSVPARTAILASADLSFRQRVKEALAGLHWQVRE
                     AAGGAEVLAHLDAAPAEAVVLDSWLPDLEIQEFMAEMEQLYPAVDLVTVDGSCAKTAR
                     SPRRNEIHYALRRAQDADGAIWNSAPVISQPEDRAPAKYVSPVRPATEILSRLPEFIG
                     QHPAMLEVSRRIRMVAPRNTAVLIQGPTGTGKELVARAIHRLSARASRPLIALNCAAI
                     PEALLEAELFGHTRGAFTGAVQGRVGRIEAAHGGTLFLDEIGEMPLPLQAKLLRFVEC
                     GELQRVGENEPVRVDVRIVAATHRTLGQLAGKGEFRADLYYRLSVFQIKTPALSVHKE
                     DIPLLVEHFLEKMAEESPAKSMTQAAIEKLLQHGWPGNVRELEHVLERAWILAEDRPQ
                     IGVAEIEFGEQAENF"
     gene            5683..6072
                     /gene="flgB"
                     /locus_tag="VFA02_00235"
     CDS             5683..6072
                     /gene="flgB"
                     /locus_tag="VFA02_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZU08301.1"
                     /translation="MQITTPVSDALERYLDLSSLELKFTAQNMANIDTPGYKTVGFDF
                     ALEMERLLNGEISAQTKNGVARKPFTAPEARVGLVDGLLERPDGNNVSMDREGLNMAE
                     AQLQFRTGVELWKREMARVMDAIHVDK"
     gene            6099..6542
                     /gene="flgC"
                     /locus_tag="VFA02_00240"
     CDS             6099..6542
                     /gene="flgC"
                     /locus_tag="VFA02_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZU08302.1"
                     /translation="MNLFGVLEVSGSALKAERIRAEVVAANMANAETTRTSDGTPYRR
                     QHVVFSSTAIEPSGFAGELMSQAEPKIGGVEVSAVVQDPASTLRRYDPGHPDADKDGY
                     VNYPDINPLTEMVDMMGATRAYGLNASAVQAEKGMLSASLDILKS"
     gene            6561..6860
                     /gene="fliE"
                     /locus_tag="VFA02_00245"
     CDS             6561..6860
                     /gene="fliE"
                     /locus_tag="VFA02_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZU08303.1"
                     /translation="MQIQSGAITSSLSSLPDAIAQKDSAQEVPFVGFFQDAMQNMQQL
                     EDKASQTVQGLLDGSGVDVHTAMIAVSNADAAFELALAVRNKGVAAFQQMMNMQF"
     gene            6909..8501
                     /gene="fliF"
                     /locus_tag="VFA02_00250"
     CDS             6909..8501
                     /gene="fliF"
                     /locus_tag="VFA02_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZU08304.1"
                     /translation="MSAARTGGLFERASTMLAPMAARFRGMERQQKIWFASAAGLAAL
                     CFIGILWFATRTDWKVLYAGLEPADAREMASELTAANIPFDVSSDGTTLRVPASSLDK
                     ARLVTTAKGGPKSGRMGFELFDKPNWIGSEFDEKVNYQRALEGELEHTINTLSDVESS
                     RVHLVMPHDSLFTSQQRDAKASVVLKLRRRSLGEDQAEAIRNLVAGAVDGLQPENVTL
                     LDADGRVQLGKRGAGAEEEAHEQALADRLIATLEPVAGVGNVRASVNVEYDTSSADEI
                     DETYDPNNVVTLSMQRSEQVSGTQTGPSGVPGTASNAPNTKPPLFPSHDAGTESARQE
                     SGTYGASKRVRHTVQNAGKIRRITAAVLINHRMNASGSQVSWQPRTPEEMKQLTELAQ
                     TAIGFDGTRGDQVSVEDVAFEDNSAPAMGPMQQFLAQAARSTDLWKYGALLIALLCLV
                     FFVVRPAMRAQKPAEKLIATPNTGAVLPQSGEGPALVEAADEAMMLEKKRAQAMFDTV
                     TEHLRQEPAQATRLLQSWIHSE"
     gene            8511..9587
                     /gene="fliG"
                     /locus_tag="VFA02_00255"
     CDS             8511..9587
                     /gene="fliG"
                     /locus_tag="VFA02_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZU08305.1"
                     /translation="MATAAVPDVTSLVPPPMPERPSALRQIPGIKKAAILVVAVGDEL
                     GKKIFQNLPDGDVQRLTEEIADLRNIPPEVSMRVIEEFYEMLETQRYMMHGGLEYATK
                     LLVESFGKQRAEDLLALVKRAQEASHGDLAMLQKVDPQQLGKFLDGEHPQTVALVLAH
                     LDPKRASLVLNGLSEEKKVAVVHRLAEMRQFSPEMAQKVAVILHKRLQTVGDTGRRAY
                     AGFKAVADLLNRLDAEQAKKILEQIEDEDPEVAINIRNLMFTFEDFVTLPLPTMRELI
                     GAIDKKVLAMALKGTREDVRAHIFRAMSSRAVEMLREDMEVLGPVRTREVASAQAEIL
                     NIARKLEADGKIVLKVEQGDDLLV"
     gene            9607..10335
                     /locus_tag="VFA02_00260"
     CDS             9607..10335
                     /locus_tag="VFA02_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HZU08306.1"
                     /translation="MLKISPMEYRSAQTLAPVLMEPKPLFPVLEPEARRTDDVAAGRE
                     KLEERVAELEQQAQLREREFAQQIEAVRKAAFEEGRNAEASLRVSAVQRIAEQMGRAL
                     EEFRNTRDGYLAQVEREVVRLALAIAERILRREAQMDPLLLAGAVRVALGQLSETTEV
                     RMKVPSSECELWSEMISLMPSLPLHPQLVADDSLHPGECLLETALGSVDLGVKSQLAE
                     IERGFFDLLEHRERAALTVRQPGP"
     gene            10336..11646
                     /locus_tag="VFA02_00265"
     CDS             10336..11646
                     /locus_tag="VFA02_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HZU08307.1"
                     /translation="MELLSTYFARLEQRSPFRWQGRVLEAVGQTIESSGPLCSVGECC
                     EIVDSEGRVHPAEVIGFRGSHVLSMPLESTEGIRFGDKVSALGTQPYLEVGDALIGRV
                     LNASGQPIDHGAVPAVRAIIPLDRNIPHPLERVPIRQPLGTGIRAIDGMLTVGRGQRI
                     GIFGGSGVGKSTLIGMMTRNSDADVIVVGLVGERGREVGEFLEDCLGPEGRKRAVVLV
                     STSDQSPLLRQRAALAATAVAEYFAEQGKNVLLVLDSLTRFAMASREIGLAAGEPPTA
                     KGYTPSVFSRMARLVERAGQFRRGSITAFYTVLMEGDDQQDPVVDAARSFLDGHVMLS
                     RALAAEGWYPPIEVLDSVSRLMPAITTEEHKTAAALVRKMLSVYAKSEDLIRIGAYKP
                     GADADLDRAIRSRALVREFLVQQASEDVSFMQGCDALKSLAVQV"
     gene            11648..>12032
                     /locus_tag="VFA02_00270"
     CDS             11648..>12032
                     /locus_tag="VFA02_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08308.1"
                     /translation="MKSSLERLHRLRGLLEGTSRIELEMRLKETALLESEALALRQEQ
                     HRALTTAGDLEAAVVAQLARWREQVIEAALLKKLQEMECAKAEYLERRKERRQVESVL
                     SERRMFESVERNRREQRTMDDWFGQK"
BASE COUNT         2634 a         2986 c         3863 g         2549 t
ORIGIN      
        1 gaggtggttt atccctcgtg gagcgcggga tttgcggcgg ctgaggagaa gaagcctaat
       61 gcgagcgcgt tgcgctggct ctggaaagaa cgctgttcct caaacgagga ggccgaccag
      121 agcggcgtgt ggcgcttccg cgagctgttg catattctgc gcgatgagaa aaacatcgtc
      181 tcactgcgcg agggaaacac gccgttgtat tcattgcggc gcacggccag atctaccggt
      241 atcgcgaagc tttatgccaa gcaccagggg atgaacccga ctgggtcctt caaagataca
      301 ggaatgacgg ctgcgctttc ggtagcgtac gagcttggat acgagtgggt ggcctgcgct
      361 tctacgggaa acacatcggc tgcgatggcg gcctatgcgg cgcgcgcagg gatgcgcagt
      421 ctggtgctga ttccgcaggg aaagattgcc tggggcaagc tctcgcaggc gatagattac
      481 ggggcgctca cgatccagct caggacagat ttcgacggct gtgtgcgcgt gctggctgaa
      541 attgtcaggc gcgcgccggt atatctgctg aattccgtca atccttaccg gctggaaggg
      601 cagaagaccc cggcccttga gattgcagaa gcattggagt gggaagtccc tgaccatgtg
      661 attgtccctg gaggcaatct tgccaacagc tctgcgctgg gcaaggcatt tctggaaatg
      721 aagcatctgg gcctgaccgc gcggcttccg aagatatccg tcattcaggc tgaaggcgcg
      781 aatcctttgt atcgcgctat gcgggaaaat gggggcgagg ccctgtatcc ggttgaggcg
      841 catacgcggg cgtccgcgat tcgtatcggc aatccggcat cgtggaagaa ggccgtgcgc
      901 gtgattcgtg aaacgggagg ctggtgcgaa caggcgagtg aggctgagat tgccctggca
      961 aaggccgaga ttggagaaga aggaattggc tgcgagccgg catcggcggt cactttggcg
     1021 ggcctgaaaa agctggtgcg cgagggcaaa gtacaatcgc aggaaacggt agtgcttttg
     1081 ttaacgggcc atacgttaaa ggattccgac tacacgatcg cttaccaccg tggtgatttg
     1141 ctccggccag acgaggtggg agcgctccgc accgaaatag aagtaacgcg ccgcaatgcg
     1201 gtggaaatgg atgcctccgc tgatctggtg ttacgcgaat tagagagggc aaaggaacga
     1261 tgagtttgcg accgatcagg ctgaaactgc ccgctacttc tgccaatctg ggccctgggt
     1321 ttgatgcgct tgcgcttgcg ctttcgttat acctggagat tgaggcagcg cccggcgaga
     1381 aatattccat agaagccagt ggacgcaatg tggacatatg cggggcgcta gaaaaaaacc
     1441 tgctgctgca cacctataaa acaattctgg agcagcaggg agtccaggaa gtaccgctga
     1501 ctttaaaggt gcgaaatggc attcctgtcg gaatggggct aggttcgtca gcggccacac
     1561 gccttgcagg ggcggccctg gcggcgcact tcggccatct tggatgggac cgcagccgta
     1621 ttctgacaga agcttcgcgc ctggaaggcc atcctgacaa tacagcggcg tgctggctgg
     1681 gaggatacac agttgcctgc tggcatggcg atgaggtagt ggcgatatcg ctcacgcccc
     1741 gcgcgtactg gcgggcattg gttgtgattc cgcagaatcc gctctctacg acactctctc
     1801 gaaaagtagt tcctgagact ttccaccgta gcgatgctgt ggcaaatatc cagcgggttg
     1861 cgctgcttac ggcggcgttt gcttccgggc acagcgatct gctgtccact gcggtcgagg
     1921 acaggatgca ccagccttac cgggctgaag tatgcccatt gctgaaaaaa ttgctgccgc
     1981 tttccgggcg ggagaatgtc ctgagcgtta cattaagcgg agcaggccca tccgtgctgc
     2041 tgctggttga gtcagatttc cagatcaacg gggtgaagga tatggtccgg cagcatctgg
     2101 agggtgagcc tgtggaaatt ctgacctgcc atatcgagct tgatccagtg gttttccaac
     2161 aaaatgattc aaattaagac atatttctat gtggttacga taatgttact taagtttgct
     2221 ttacatcacc gacttaagag attagcttgc aattcgtggg cagttctggg ggagggctgc
     2281 tctagcgcct gagactccga tgtatagagt ccaggcgctt ttactttgct ccctcttccc
     2341 attgaggtcc ctgttgcgac cttacaggag catttatcat caaatggagt gaatttctgg
     2401 ggacccagta aaggaggagc atggcaggtc tggcagtagt tgaggtgaat gatacaaatt
     2461 ttgagcagga ggtgctccag tcagatcagc ctgttctggt agatttctgg gccgcctggt
     2521 gcgggccctg caaggcactg gcgccgattg tggatgaagt tgcggcggaa tatagcggca
     2581 agctcaaggt tatgaagatg gatgtcgatc gcaatcaggc gacaccgatg cgctacggta
     2641 tccgcggcat tcccgcgctg ctgctcttca agggcggcaa agtcgccgac cagatcgtag
     2701 gctatgttcc gaaagacacg attgcccgca gcattgcgcg cgtgatcgga taatctaaaa
     2761 cgagtccagg aagacacaag gcctgccgct gtgcaggcct ttgtatttca ggcatacctg
     2821 cgcaggacgc cgagcgcgcg tccctgaatc gcaacctggt ccgcgggaac ataaatgggt
     2881 tccatctctg cattggaggg ctgaagacga accatcgggc cctcgatgta atagcgctta
     2941 agcgtggttt ccgcgccatt taccagcgcg actacgatct ctccctggtg cgcggtgcgg
     3001 gtcttttcca ccagaacata atcaccgtca acaatatgct cgtcgcgcat agaatcacca
     3061 cgtacctgaa gcacaaacac atcccgattc cctatgatgt cagacagaga gatgctctcg
     3121 gcggtctcag gtgcttctac gggaaagccg gcggcgatgc gtccggccag cggcaggcgg
     3181 tctgggaccg ctgacttact gcgaggcgga agcacatcaa tggagcggct gcgattgtgc
     3241 gcccgttcaa gaagcccttt gctttgcaga ttggtgatgt gcttatggac cgtagcgaga
     3301 gacttgaggc ccaaaccccg ggcaatctct tcaaaggaag gtgagtagcc gttgcgctgc
     3361 acaaagccgg agatgaaatc cagcacttct ttttgccgcc gagtgactgc catgcccgta
     3421 ttgtagcgaa taaaaagcga atttccagaa aaaagttaca aagatatgca aaagcggcga
     3481 aatttttcaa tccatagctg tcaagacccg gaagcaaaaa tccttgtgcc tagtttcatt
     3541 ttgagacgca aaatgctctc attcccaatt caaaatcaag accgtttttc tgcttaagca
     3601 tttcttgtgt tccacccgcg ttacaggcga agttagggaa gcgcgaggga attccaggat
     3661 gcggttctta gtagcggatg acgatcaggc gctggcgcgg tttctgcgcc gcgggtttga
     3721 agctgacggg caccaggtca tgcttgctcc ggatggccag acggcgatgg agatgttcgt
     3781 gtcggaatcg cccgacctga ccgtgcttga tctggacctg ccgcgcaaag atggaatcga
     3841 agttctgagg tttttacgtt ctgtcagcga tgaggctccg gtgctggtgt tgacggcgag
     3901 gaacgagatg gaaaccagag tgagatgtct tgaaatgggc gcagatgatt atgtgctgaa
     3961 gccattctct ctgcgcgagc ttcgcgcccg ctgccgctct ctcatgcgtc gcagacaggg
     4021 agcaggagtg gtgctgcgct atgccgatat tgaggtgaat cgggtggagc gcacagtggt
     4081 gcgtgccgga gatgccgtgc agcttaccaa caaggaattt gcgcttctgg agtttctgct
     4141 gctcaatcgt gggcgctgcg tctcgcgcgc tgaattgttg caacgggtat gggagatgag
     4201 cccggaagcc ggcgccaatg tagtggatgt gtatgtaaat tacctgcggc gcaagctgaa
     4261 gaatgcaggc gggccttcgc tgatccagac cgtgcgcggg catggttatt cagtcggaat
     4321 gaagaccaac tcataaaaat aaattgctga ggaacactga tgtctactgc cgctctcgta
     4381 aaacgctccg tttctgttcc cgccaggacc gccattctgg ccagtgctga cctttctttt
     4441 cgccagcgtg tgaaggaggc gcttgccgga ttgcactggc aggtgcgcga agctgccgga
     4501 ggcgcggaag ttctggccca tctggatgcc gctccggcag aagctgttgt gctggattca
     4561 tggctgccag acctggaaat acaggagttc atggccgaaa tggaacaact ttatcctgct
     4621 gtggacctgg tgacagtgga tggcagctgt gcaaagacgg cccggagtcc gcgccgaaac
     4681 gagatccatt atgctcttcg gcgggcacaa gatgcagatg gggccatctg gaacagtgcc
     4741 cctgtgattt cgcagccgga ggaccgtgcg ccggcaaaat atgtaagtcc agtaaggccg
     4801 gccactgaaa ttttgagcag gctgccagag tttattggcc aacaccctgc catgcttgag
     4861 gtcagccgcc gcatccgtat ggtcgcaccg agaaatacag cagttttgat tcaaggcccc
     4921 accggaacag gaaaagaact ggtggcccgc gctattcacc ggttgagcgc tcgggcctcg
     4981 cgcccgttga ttgcgttgaa ctgcgcggca attccagaag ctttgctcga agccgagctt
     5041 tttgggcaca cccgcggagc atttaccggg gccgtacagg ggcgtgttgg gcgcattgag
     5101 gccgcgcatg gtggcacgct ctttctggac gaaattggcg agatgccatt gcccttgcag
     5161 gccaaactgc tgcggtttgt ggaatgcggc gagttacagc gggttgggga gaatgagccg
     5221 gtccgcgtag atgttcgcat cgtcgctgcg acacatcgta ctttgggaca gcttgccggc
     5281 aaaggggagt tccgcgcaga cctttactat cgcttgtctg tcttccagat taaaacaccc
     5341 gcgctttccg ttcacaagga agacattccg ctgctggtgg agcactttct tgaaaaaatg
     5401 gcggaagaaa gcccggcaaa atccatgact caggcagcga tagaaaagct gttacagcat
     5461 ggctggccgg gaaatgtccg cgagctggag cacgtgttgg agcgcgcctg gattttggcc
     5521 gaggacagac cacagattgg tgttgctgag atcgagtttg gcgagcaggc ggagaatttc
     5581 tgagattttc ctaatctgac tcccggcaac gcgggccgtc ccatgcaact gctgaaagcg
     5641 cgcccgctct acttatcggc agatgcggag aaaggatgaa cgatgcagat tacgactcct
     5701 gtaagtgatg cattggaacg gtatctcgac ctctcgagtc tggagttgaa atttacagcg
     5761 cagaacatgg cgaacattga tacgcccgga tacaagaccg tggggtttga ttttgctttg
     5821 gagatggagc ggttgctgaa tggggagatt tccgcgcaga caaaaaatgg cgttgcgcgc
     5881 aagccgttta cagccccgga ggcgcgtgtg ggcctggtgg atgggctatt ggagcgtccg
     5941 gatgggaata atgtttcgat ggaccgggaa gggctgaata tggcagaggc gcaattacag
     6001 tttcgcaccg gcgttgaact gtggaagcgc gagatggcgc gcgtgatgga tgccatccat
     6061 gtggacaagt aatccgggca tttgcgaaag gagaagcgat gaatctgttt ggagttctgg
     6121 aagtgagcgg ctcggcattg aaggcggagc gtatccgggc ggaggtggtg gcggccaaca
     6181 tggccaatgc agaaacaaca cgcacatcgg atggtactcc gtaccggcgt cagcacgtgg
     6241 tcttttcttc cacagccata gagccatcag gatttgccgg agagctgatg tcgcaggctg
     6301 agccgaaaat tggcggcgta gaagtctcag ccgttgtcca ggacccggcg agcacgttgc
     6361 gccgttatga tcctggacac cccgacgcgg acaaagacgg gtatgtgaac tatcccgata
     6421 ttaatccgct gacggagatg gtggacatga tgggggcgac gcgcgcttat ggtttgaatg
     6481 cctcggccgt tcaggcggag aagggcatgc tcagcgcgtc gctggacatt ttgaagtcgt
     6541 agtgaatttt gaggacatcc atgcagattc aatcaggagc aatcacaagt tctctctcat
     6601 ctttgcccga tgcaattgcg caaaaagata gtgcacagga agtgccattt gtcgggttct
     6661 ttcaagatgc aatgcagaac atgcagcaac tggaagacaa ggcatcccag accgtgcagg
     6721 gactgcttga tggcagcggt gtggacgtgc atacagcgat gattgccgtc agcaatgccg
     6781 atgcagcctt tgagcttgcg cttgcagtac gcaacaaggg cgtggccgcc tttcaacaaa
     6841 tgatgaacat gcagttttaa ggaaaagcgg gacagcgcag catggaagag aaccagcaga
     6901 tacaacctgt gtccgcggcc agaacgggcg gactgttcga gcgtgcaagc actatgctcg
     6961 caccgatggc ggcgcgtttt cggggcatgg agcggcagca aaagatctgg ttcgccagcg
     7021 ctgccgggtt ggctgcgctc tgcttcattg ggattttgtg gtttgcaaca cggacggact
     7081 ggaaagtgct ttatgccggg ttggagcctg cggatgcgcg tgagatggct tcggaactga
     7141 cggcggcgaa cattcctttc gacgtttcat ccgacggtac gactttgcgc gtgcctgcgt
     7201 ccagcctgga caaggcccgg ttggtaacca cggccaaagg cggcccgaag agcggacgca
     7261 tgggcttcga gcttttcgac aaacctaact ggattggctc cgagtttgac gagaaggtga
     7321 actatcagcg cgcgctggaa ggcgagctgg agcataccat caatacactt tccgatgttg
     7381 aatcttcaag ggtgcatctg gtaatgccgc acgattcgct ttttacgagt cagcagcggg
     7441 atgcgaaggc atcggttgtg ctgaagctgc gccgccgttc cctcggagag gaccaggcgg
     7501 aagccattcg taatctggtg gcgggcgccg tggatggtct tcagccggag aatgtcacgc
     7561 ttctggatgc agatgggcgt gtgcaacttg gcaagcgtgg ggcaggcgcg gaagaagagg
     7621 cgcacgagca ggccctcgca gacaggctga ttgctacgct ggagccggtg gcgggagtgg
     7681 gcaatgtgcg ggcctcagtg aacgtggagt acgacacctc ttctgccgat gaaatagatg
     7741 agacctatga tccgaacaat gtagtaacgc tttcaatgca gcgttctgag caggtttccg
     7801 ggacgcagac agggccatcg ggtgttcccg gcacagcaag taatgcacca aatacaaagc
     7861 cgccgctgtt tccatcgcat gatgccggaa cggaaagcgc gaggcaggaa agcggcacct
     7921 acggcgcttc aaagcgggtg cggcatacgg tccagaacgc gggaaagata cggcgcatta
     7981 cagcagccgt gcttattaat caccggatga atgcaagcgg gagccaggtc agctggcaac
     8041 ctcgcactcc ggaagaaatg aagcaactga cggaactggc acagacggcc atcggattcg
     8101 atggtacgcg tggtgaccag gtgagtgtgg aagatgttgc ttttgaggac aactcggcgc
     8161 cggcaatggg tccgatgcag cagtttctgg cgcaggcagc gcgttccact gacctttgga
     8221 aatatggcgc attgctgatt gcgctactat gcctggtctt ctttgtagtg agacccgcaa
     8281 tgcgggcaca gaagcccgca gaaaagctga tcgcgacgcc aaatacaggc gcggtactgc
     8341 cgcagagcgg agaggggcca gcactcgtgg aagcagccga tgaagcgatg atgcttgaga
     8401 agaaacgggc gcaggccatg tttgatacgg tcacagaaca ccttcgtcag gaaccggcac
     8461 aggcaacacg cctgctgcaa agctggattc attcggaata ggagaaaacc atggcaactg
     8521 ctgctgtccc agacgtaacc agcctggtac cgcctccgat gccggaacgc ccctctgcct
     8581 tacggcagat acccggcatc aagaaggcgg ccattctcgt agtggctgta ggcgatgaat
     8641 tgggcaagaa aatctttcag aaccttccgg atggcgatgt gcagcggctg accgaggaaa
     8701 ttgctgattt gcgcaatatt cctcccgagg tctcgatgcg tgtgatcgaa gagttctatg
     8761 aaatgctcga gacccagcga tacatgatgc atgggggcct cgagtatgcg acgaagctgc
     8821 tggtcgagtc ttttggcaag cagcgagcag aagacctgct ggctttggtc aaacgagcgc
     8881 aggaggcgag ccacggggat ctggccatgt tgcaaaaagt ggatccgcag cagcttggga
     8941 agtttcttga tggtgagcat ccgcagacgg tggcgctggt gctggcgcat cttgatccca
     9001 agcgggcttc tctggtcctg aacggcctca gcgaggaaaa gaaggtcgct gttgtgcacc
     9061 gtttggcgga aatgcggcag ttttcgccgg agatggcgca gaaggttgcg gtgattctgc
     9121 acaagcgtct gcaaactgtg ggcgacaccg gacgcagggc ctatgccggg ttcaaggccg
     9181 ttgccgattt gttgaaccgt ctggatgccg agcaggcgaa gaagatcctg gagcagattg
     9241 aagatgaaga tccggaagtg gccatcaata ttcgtaatct gatgttcacc tttgaagact
     9301 tcgtgaccct gccattgccc acgatgcgtg aactcatcgg tgcaattgat aagaaagtgc
     9361 tggcgatggc gctgaagggc acacgtgaag atgtgcgcgc tcatatcttc cgggccatgt
     9421 cgtcgcgcgc tgtggagatg ctgcgcgaag acatggaagt cctggggcct gtgcgcaccc
     9481 gtgaagtggc gtcggcacag gcagagattc tgaacattgc gcgcaagctt gaggctgacg
     9541 gaaagatcgt tcttaaggtt gagcagggcg atgaccttct tgtttagctg aatgaccgga
     9601 gagagaatgc tgaaaatcag ccctatggag taccgatctg cgcagacgct ggcgcctgtg
     9661 ctgatggagc ctaaaccgct gtttcctgtt ctggaacccg aggcccgtcg taccgacgac
     9721 gtggccgccg ggcgcgaaaa actggaggag agggtcgcgg aactggagca gcaggcccag
     9781 ttgcgggaac gcgaattcgc acagcagatc gaggcggtgc ggaaggcggc ctttgaggaa
     9841 ggccgcaatg ccgaggcttc gctccgtgtg tcggctgtgc agaggattgc cgagcagatg
     9901 ggccgtgcgc ttgaggagtt tcgtaacacg cgcgatggct atctggcgca ggttgagcgt
     9961 gaggtggtgc gtctggcgct tgcgattgcc gaacggattc tgcgacggga ggcgcagatg
    10021 gacccgcttc tgcttgcggg tgcggtgcgc gttgccctcg ggcagctttc tgaaacgacg
    10081 gaagtccgca tgaaagtgcc ttcatcagag tgcgagctat ggagcgagat gatcagcctg
    10141 atgccttcgc tcccgctcca tccacaatta gtagctgacg attcattaca tcccggtgaa
    10201 tgcttgctgg aaacagcgct gggcagtgtg gacctcggag tgaagtcgca gctggcggag
    10261 attgaacgag ggttttttga tctgttggag catcgcgagc gggcagcgct gaccgtgagg
    10321 cagccaggac cataaatgga actactgtct acatattttg cgcggttgga gcagcggtca
    10381 cccttccgct ggcaaggccg cgtgctggag gcggtggggc aaaccatcga atcctctgga
    10441 ccactctgct ccgtaggaga gtgctgcgaa attgtggaca gcgaaggacg tgtgcatccg
    10501 gcggaagtga ttgggtttcg tgggagccat gtgctttcga tgcctcttga aagtacagaa
    10561 ggcatccggt ttggcgataa agtttcggcg ctggggacgc agccttatct ggaagttgga
    10621 gatgcattga tcgggcgcgt cctgaacgcc tccggccagc ccattgatca tggggctgtt
    10681 cccgcagtcc gggccatcat tcctctggac cggaacatcc cgcatccgct ggagcgcgtg
    10741 ccgatacgcc agcccctggg cacgggcatt cgcgcgattg acgggatgct gacagtaggg
    10801 cggggacagc gcattggcat ctttggcggc tccggcgtag gcaagagcac gctgattggc
    10861 atgatgacac gcaacagtga tgcagatgtg attgtagtcg ggctggtagg tgaacgcgga
    10921 cgcgaggtgg gagagtttct cgaagactgc ctcgggccgg agggaaggaa gcgggccgtt
    10981 gttctggttt cgacgtctga tcagtctccg ttgctccgtc aacgtgctgc tctggcagcc
    11041 acagcggtcg cggaatactt tgccgaacaa gggaagaatg ttcttcttgt tttggattct
    11101 ctgacgcgct ttgccatggc ctcgcgtgag attggccttg ccgcaggaga gcctccaacg
    11161 gcaaaagggt atacgccttc tgttttcagc cgcatggcgc gattagtgga gcgggctggg
    11221 cagttccgcc gtggaagcat cacggcgttt tacacagtgc tgatggaagg ggatgaccag
    11281 caggaccctg tggtggatgc agccaggtca tttctggatg ggcacgtgat gctttcgcgc
    11341 gccctggctg cggagggatg gtatccaccg attgaggtgc tggattctgt gagccgtctt
    11401 atgccagcca tcacaacgga ggaacataag acagcggcag cgttggtgcg gaaaatgctt
    11461 tcggtgtatg cgaagtcgga agaccttatc cggatcggag cttacaagcc aggcgcggat
    11521 gcagatctgg accgtgctat acgatcgcgc gctctggtgc gcgaatttct ggtgcagcag
    11581 gcttccgaag atgtttcttt catgcaggga tgcgatgctc tgaaatcgct ggcagtgcag
    11641 gtgtgatgtg aagagttcgc tggagcgtct gcacaggcta cggggcctgc tcgaaggtac
    11701 gagtcggata gagctggaga tgcggctgaa ggagacggcc ttgctcgaaa gcgaagcact
    11761 cgcgttgcgg caagaacagc accgggcgct taccacagcc ggggacctcg aggcggctgt
    11821 tgtggcccag cttgcccggt ggcgagaaca ggtcatcgaa gccgcattgc tcaaaaagtt
    11881 gcaggagatg gagtgtgcaa aggctgaata ccttgagcgc cgcaaggagc gacgccaggt
    11941 ggagagcgta ctttctgagc gacggatgtt tgagagtgtt gagcgtaacc ggcgggaaca
    12001 gcggaccatg gatgattggt ttgggcagaa aa
//
LOCUS       DASRBV010000011        10499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_304443, whole genome shotgun sequence.
ACCESSION   DASRBV010000011 DASRBV010000000
VERSION     DASRBV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304443"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..721)
                     /locus_tag="VFA02_00275"
     CDS             complement(311..721)
                     /locus_tag="VFA02_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08309.1"
                     /translation="MVRRCKIPAVVIVFFLSVTTILFADHGQGHGRGKGHNKSSFVYF
                     RQQDYGPLVSYYQGPRNLPPGLAKKYYRTGTLPPGWQKKLQPFPPALVQQLPPLPPGC
                     NCAMGYVDGQAIVYDTTTRVILDSVDLISALSGH"
     gene            complement(912..2027)
                     /locus_tag="VFA02_00280"
     CDS             complement(912..2027)
                     /locus_tag="VFA02_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZU08310.1"
                     /translation="MKHSLRLGWTILMAGGAALVLSTNPGLAQPTTVFNDATAYLAHS
                     SQGYLGVELHDIDNERATSLKLKDVHGAEIVTVDHDGPANKAGLRPHDVILEMNGQRV
                     EGVEQLKRMLHETPAGHTVHFVVSRDGQQKEISVELADRSKVEAEAWSKHFSVPDPEE
                     APLPDGFLAPSTSHGFGSGFFGVFTGNSLYVGAEVDAISTQLANYFGVTDGSGLLVKS
                     VDENSPAAAAGLKAGDVITKVNNERMASRSDWFKALHNNRGKQVQLTVMRDKKEQKLT
                     MQAGESKKKGEMDWPCFTSGSQQLTAELNRQFAVLAPEINRATAAAMAAQADAIAKSI
                     DTKKLSDEIQKEINSKDIQKMQQELEQLQEQLHHDMN"
     gene            complement(2112..3095)
                     /locus_tag="VFA02_00285"
     CDS             complement(2112..3095)
                     /locus_tag="VFA02_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZU08311.1"
                     /translation="MAMKCDLAQQHIALAVYGELSDDASHQLEQHLKECEDCRREMEA
                     VRGLQAAMALYPVEEPSPSLIARARMRLDEALDTIPHSGWLERTVQLFSRGVGRLRSA
                     PVMASLLLFAGVSMGGYGGYRAGLNAHDAQQAKAVSTAVHTQSANDDASVQVANVSNI
                     TRDPNSNLITVSYNKLVPETMQGPPDDPRIHQLLLLGTQNSVNPGVRDNSVDLLANEC
                     RAGRQCDDGRVRDALMVALRHDKNASVRMKALEGLQPYIAEDMRVRDAVLETLMKDSD
                     PRVRTQAINLIEPVEADSSVREVLHTVAMQDDSPEIRTASRQVLQQMPQIQ"
     gene            complement(3229..3825)
                     /locus_tag="VFA02_00290"
     CDS             complement(3229..3825)
                     /locus_tag="VFA02_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08312.1"
                     /translation="MPTEVRDRTTETELIREAQQGSRAAFDILVRQYDQAVLRLALHL
                     TGSEQDAQDIHQEAFLKAFRYIGNFRFECSFYTWIYRIVTNLCLDQLRRRKSRREDPA
                     IMIDSSGEEMDLLGNVSDDRSIANPDRELQRKILGQRIQAALEKLTPRERMVFELKHY
                     QGLKLRTIGEMLNTTEETAKNTLFRATKKLRASLAEMR"
     gene            4007..4591
                     /locus_tag="VFA02_00295"
     CDS             4007..4591
                     /locus_tag="VFA02_00295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HZU08313.1"
                     /translation="MSSEREGELFSASNVSGEEKSWITAYCDGGSRGNPGPAGYGVYL
                     EDAQGRKLDELSEFLGRKTNNFAEYSSLLAALDYALQHGITHLKVIADSELLVRQING
                     QYRVNSPELRPLYEEALHRIRQLKGFEIKHVLREKNQNADRLANLAMDKGTGRSSPTA
                     PPQKTMRGFVKGGVIHLLDGELPDGVFVEIYPRR"
     gene            complement(4595..5983)
                     /locus_tag="VFA02_00300"
     CDS             complement(4595..5983)
                     /locus_tag="VFA02_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU08314.1"
                     /translation="MPATQETKQTRELPRVLSASHATAIVVGTIIGSGIFLVPHEMVR
                     AVGSSGLVYLAWVAGGLLSLFGAMTYAELGAMMPYAGGEYVYIRNAYGDMPAFLYMWA
                     WFSVAKPASIASVTSGLARTMAIFGPFAWLERPVLHNSEALYWSQLFAVAATWLITGL
                     NYLGMKKAGDFQLIFTWLKAVLIAVIAGFCFSSHSGTLINFKTAFTGAQGGFGGFMVA
                     MIAVLWAYDGWNDLTMVAGEVKRPERSLPIALIAGLFIVGALYMTTNAAIQYILPATQ
                     IADSPRPAVAAMAIVSGHAGAVLVSAAMALSIFVTLNGTIMSGARIPFAAARDRLFFQ
                     RMARVHPRFQSPSTSLVVQGLLSTLLLLVVGRFQQLFELAIFAEWLFYMVTATTIFVY
                     RKRLPDAPRPYRVWGYPMLPALFIAASAVLLYFSYVENLRNSLIGTAIIAAGIPLFGL
                     IRRHYGESSSSL"
     gene            6450..9842
                     /locus_tag="VFA02_00305"
     CDS             6450..9842
                     /locus_tag="VFA02_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08315.1"
                     /translation="MVVTSGMARGQTISGTITGTVQDSTGAVVPNATITITNSGQNVT
                     VFTGKTNSAGQYTAPFLPVGNYAVSAEAPGFKKVEHTGLKLNVNQDLTVDVVLEPGSA
                     QQTVNVSASALQVDLQSAQAQTVINGTQIRQLPLTTRNYEQLVALMPGVSTGLASDQL
                     YVGASNPVGTSNQINFSINGSRPTQNTWNIDGADNVDRGANLTLLAYPSVDSIAEFSV
                     QRSNYNAEFGRSSSGQINVITNAGTDQFHGDVYEFFRNDVLAANTYLNNRKQLRIAPL
                     RYNDFGGTIGGPIWKDHTFFFFSEEVRRVITYTNFIASVPSLAELKGDLSAAANPTAS
                     GKPGPGPVCVQWSAPGGTCLQQGYQVTNISPAAQAYIKDIFSKLPVPDNPNCTGGCTL
                     TSSGRNIFNMRQEIVRIDQVFSPKFRIFGRFENDTIPTTEPGGLFIGSPLPGVATSST
                     NSPGRIFTIHATNAISPTLINDAGYNYSHGGILSTPIGLTAGKNSPDIASAVKLPYTS
                     SLNVAPVLGFTYFSNVSDRGQYRDFNDNNNAFDHLSKVAGRHSLKFGVSYNWYQKDEN
                     SASGNQGNFGFPITAPNGNNSEYQEWANFLTGNASSFGQASVDFRAVVRQQQWEMYAQ
                     DTFQIRPNLTLSYGLRYSLFRQPTDAKEHLTNFDPATFDPAGAPPIDPNTGKIAGTGT
                     PLNGIIVNGKSSPFGDAVNQQNDLDFAPRVGFAWDPIGKGQMSVRGGYGMFYDSSAVS
                     RYEINVFTNPPFVNSLSLGPTLLDNPSGAAIVSSYPSPLAALGVKWHTPYSQEWNLDL
                     QQQLPAQWLFDIGYYGGVGKHLLGEVDLNQPLPGAYVTALAPYGVKGPPTRGKTTQQL
                     NYIRPYKGYDAINTQITEYMSNYNGLQVALQKRFGASSLLNVNYTWSRAMTNAFNDTT
                     AAQNTYDLKAEYGPARYDRRNIFNANFVYGLPFFQTQSGLVGHVLGGWELTGIAYAYT
                     GLPYTVTGVLNDPAGQGVLDGNSAVSGRPDLVGNPNQANAVSGPIHNNNGIQPWFNTS
                     AYAQVCPTVSGVTTCPTGARPGNSHPGSVRGPGLWRGDLSLHKNTKITETVNLQFRAE
                     ASNVFNHTNPDGISTSYTSSLFGKVTSYRDPRILQLALKLNF"
     gene            complement(10214..10489)
                     /gene="rpsT"
                     /locus_tag="VFA02_00310"
     CDS             complement(10214..10489)
                     /gene="rpsT"
                     /locus_tag="VFA02_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZU08316.1"
                     /translation="MANHVSALKRARQTERKTAVNRANKSRVRGAIRSLREALAKGDV
                     AAAHEQFRKTVSQIDKSVQKGILHDNTASRYKSRLAVRLKALSAKAA"
BASE COUNT         2465 a         2850 c         2784 g         2400 t
ORIGIN      
        1 acctcgtcgt actcattgca gtaggcacac gaaagattgc accggcgcat aggaacaatc
       61 tgcgccatca ccggatgcct tgtcgaaagc aacgcatgac ccaccagaga caactccctt
      121 acccggcggc tggcggcttt cattcggtat ctcaggttct tttgccgtgc tgctgacata
      181 tacctttctt attgaaacac agagagatag gaggaggcga ccctgccccg tggagcagcg
      241 tcggttctgg cccgaacggg taaagataag gccccaatgg aaaacaccgc cgggcccata
      301 tctgtatggt tcaatgtccg gaaagtgcgc tgatgagatc tactgaatcg agaatcaccc
      361 gtgtagtcgt gtcatagaca atggcctggc cgtctacata tcccatggcg caattgcatc
      421 cggggggaag cggaggcagt tgctgcacca gtgccggagg aaagggctgg agctttttct
      481 gccaccccgg cggcaatgtg cccgtccggt aatatttctt cgccagcccc ggaggtagat
      541 tgcgcggccc ctggtagtaa ctcaccaatg ggccataatc ctgctggcgg aaataaacga
      601 agctgctctt gttatgccct ttgcctctgc catgtccttg tccgtggtcg gcgaagagga
      661 tggttgtgac tgaaagaaaa aacacgatta ctacagctgg aattttgcag cgtcttacca
      721 tctgtaccct ccagaaagct caatggaaga acattatagg ggtatacgcg gacgatgggg
      781 aggcaaaatg aagtagggct gagtgcgaaa cactaaaccc tgccaacctg gttttgtacg
      841 gagggaatgt tccggagcaa caattgcttg ctggtctata gtttgcccaa acaaattttt
      901 gaagaaccaa attaattcat gtcgtggtga agctgctcct gcaactgctc aagttcctgc
      961 tgcatcttct ggatgtcttt tgagtttatt tctttctgga tttcatcgga gagcttcttt
     1021 gtatcaatgc ttttcgcgat ggcgtcagcc tgcgctgcca tggctgcggc cgtggcgcgg
     1081 ttgatctccg gggcgaggac cgcgaactgg cgattcagct ctgcggtcaa ctgctgcgaa
     1141 ccagacgtga agcagggcca atccatctcg cctttcttct ttgattcgcc cgcctgcatc
     1201 gtcagtttct gctctttttt gtcgcgcatg acggtaagct gcacctgctt gccgcgatta
     1261 ttgtgcagcg ccttgaacca atccgaccgc gaggccatgc gctcgttgtt cactttcgtg
     1321 atcacatcac ccgctttcag tcctgccgct gccgccggac tgttctcgtc cacgctcttt
     1381 accagaagac ctgacccgtc tgtcacgcca aagtagttgg ccagttgcgt gctgatcgcg
     1441 tctacttccg cacccacata gagactgttt ccggtaaaga caccgaagaa cccgctcccg
     1501 aacccatgcg aagtagaagg ggccagaaat ccgtcaggaa ggggcgcttc ctccgggtca
     1561 ggcaccgaaa agtgcttcga ccatgcttcc gcttccactt tggagcggtc tgccagttct
     1621 acggaaatct ccttttgctg cccatcgcgg ctcaccacaa aatgaacggt atgtcctgcg
     1681 ggagtttcat ggagcatgcg cttcagttgc tcaacacctt ccacacgctg cccattcatt
     1741 tccagaatga catcatgcgg cctcaatcct gccttgttgg cggggccatc atggtcaaca
     1801 gtcacaattt ctgctccgtg aacatccttc agcttcagcg acgttgcccg ctcattatct
     1861 atatcgtgga gctcaacgcc aagatatccc tgtgaactgt gcgccagata tgccgtagcg
     1921 tcgttgaaaa ctgttgtggg ctgcgccagc cccggatttg tgcttaagac aagcgctgca
     1981 ccgcctgcca tcaggatcgt ccaacccagc ctcaaagaat gtttcataag agccgccttg
     2041 tttttctgct tacttgttat gtagactcgc ccgctgtgga aaaagtcacc cggtcgaact
     2101 ccggctgaag cctactggat ctgcggcatt tgctgcaaga cctgccgcga ggccgtgcgg
     2161 atttccggac tgtcgtcctg catggcgacc gtatgcagga cctcacgcac actcgagtca
     2221 gcttccaccg gctcgatcag attgatggcc tgcgtgcgga cccgagggtc agagtccttc
     2281 atcagggtct ccagaacagc atcacgaaca cgcatatctt cggcaatata gggctgaagt
     2341 ccttcgaggg ccttcatgcg aacactcgca ttcttgtcat gccgcagcgc aaccatcagg
     2401 gcatcgcgca cacgcccgtc atcgcactgg cgcccggcgc gacattcatt cgccagaagg
     2461 tctaccgaat tgtcgcgcac tcccggattc acactgttct gcgttcccag aagcagcagc
     2521 tgatggatgc ggggatcgtc cggcggccct tgcatcgtct ccggcaccag cttgttgtag
     2581 ctcaccgtta tcaggttcga gttcgggtca cgcgtaatgt tgctgacgtt tgcaacctgc
     2641 acggaagcat catcgttggc agattgcgta tgcaccgcag ttgaaacggc cttggcctgc
     2701 tgcgcatcgt gcgcattcag tcccgcgcga taaccgccat atccgcccat cgaaacacct
     2761 gcgaaaagca gcagtgaagc catgacgggc gcggaacgca gccggcccac gccccggcta
     2821 aaaagctgga cggtgcgctc cagccatccg ctatgaggta tcgtatccag cgcctcatcc
     2881 aggcgcatgc gcgcccgcgc gatcagactg ggcgacggtt cctcaacggg atagagcgcc
     2941 atggccgcct gaaggccgcg gacggcttcc atctcgcggc ggcagtcctc gcattccttc
     3001 aggtgttgct cgagttggtg gctggcgtca tcggagagtt ctccgtaaac agccagcgca
     3061 atgtgttgtt gagccagatc gcacttcatg gccattgttc ctgtacctca ctgtcctcaa
     3121 aaagtgttgc ttccttcttt tttcctgccg gctttccgcc agtctttctt acatatgcat
     3181 cctggtcgta aaggacatgc atttcttcgc aggtcccggg tgccccaatc aacgcatttc
     3241 tgccaaactg gcccttaact tcttcgtcgc ccggaagagc gtattctttg ccgtttcttc
     3301 cgtcgtattc agcatttctc ctatcgtgcg cagcttcagc ccctgataat gcttcaattc
     3361 aaagaccata cgctcacgcg gcgtcagctt ttcgagcgcc gcctgaatgc gctggcccag
     3421 aatcttccgt tgcagctccc ggtccggatt cgcaatcgag cggtcgtcgc tcacattgcc
     3481 gagcaggtcc atctcttccc cggacgaatc aatcatgatc gccgggtctt cccgccgcga
     3541 cttgcgccgc cgcaactggt ccaggcacag gttcgtcaca atccggtaaa tccacgtgta
     3601 gaacgagcac tcaaaccgga agttgccgat ataccggaag gccttcagga acgcctcctg
     3661 gtggatgtcc tgcgcgtcct gttctgagcc ggtcaggtgc agggccaggc gcaacaccgc
     3721 ctgatcatat tgacgcacca gaatgtcaaa ggcagcgcgc gagccttgct gggcttcgcg
     3781 aatcagctcg gtttcagtgg tccggtcacg tacttcagtt ggcatccgtt gcgaggattt
     3841 taccgctcgc gccgcctcgt gtgccgtagc attggtgggg acggggatgc gtagtgcgtc
     3901 tgcagccatg cttcctactc cagaaaaaag caatgcaaat gcgatatata agacgtcctg
     3961 gcgaaggaaa ggtgagcgga atctatcact tatttacact ctttctatga gcagcgaacg
     4021 cgaaggcgaa cttttttcag cttccaatgt ctcaggcgaa gaaaaatcct ggattaccgc
     4081 atactgcgat gggggttcac gcggtaatcc aggcccagcg gggtatggtg tatatctgga
     4141 agacgcacaa gggcgcaaat tggacgaatt aagcgagttt ttgggcagaa aaacgaacaa
     4201 ttttgcagaa tattcaagtc ttctggctgc cctcgactat gccttgcagc atggaatcac
     4261 ccacctgaaa gtcatcgccg actccgagct gctggtaaga cagatcaatg gtcagtaccg
     4321 ggtcaacagc ccggaactgc ggccgttata tgaagaggcc ctgcaccgga ttcgccagct
     4381 caagggtttt gagatcaagc atgtgcttcg ggaaaagaac caaaacgctg accgtctggc
     4441 aaacctggcg atggacaaag gcacggggcg ctcttcccca accgcgccgc cacagaaaac
     4501 gatgcgcggt ttcgtaaaag gcggagtcat ccatctcctt gacggagaac tgcccgatgg
     4561 ggtgtttgta gaaatctatc ccaggcgata aaaatcaaag agatgaagag gactcgccgt
     4621 aatgcctgcg gatcaggcca aatagcggaa ttccagcggc aataatagcg gtaccgatga
     4681 gcgaattgcg cagattttcc acatatgaga aatagagcag aacagcggag gccgcgataa
     4741 aaagcgcagg cagcatagga tatccccaca cccggtaagg gcgtggcgca tccggtaggc
     4801 gcttgcgata gacgaaaatg gttgttgccg taaccatata aaaaagccac tcggcgaaaa
     4861 ttgccaactc aaaaagctgt tgaaaacggc ccacaaccag cagcagcagt gtactcagca
     4921 gtccctggac tacgagcgag gtggaagggc tttggaagcg gggatgcaca cgcgccattc
     4981 gttgaaagaa gaggcgatcg cgcgcggcgg caaaaggaat gcgcgctccg gacataattg
     5041 tgccgttcag agttacaaat atgctcagcg ccatagcggc tgataccagc accgcgcccg
     5101 cgtgtccgga gacaatagcc atggctgcca ctgcaggacg gggagagtcg gcaatctgcg
     5161 tggcaggcag aatgtactgg atggccgcgt tggtcgtcat gtatagcgcg ccgacgatga
     5221 acaatccggc gatgagggcg ataggcaggc tgcgttccgg ccgtttgacc tcgcccgcaa
     5281 ccattgtcag atcattccag ccgtcatagg cccagagtac ggcgatcatg gcgaccataa
     5341 acccgccgaa tccgccctgg gcgccagtaa acgccgtttt gaagttgatc agtgtgccgg
     5401 agtgcgagct aaaacagaat ccggcaatca ccgcaatcag cacggccttc aaccacgtga
     5461 atataagctg gaaatccccg gctttcttca taccgagata gttcagtccc gtaataagcc
     5521 atgtggccgc gacagcaaag agttgtgacc agtagagggc ctcggagttg tgaaggacag
     5581 gcctttccag ccatgcaaag ggcccaaaga tggccatggt gcgcgcaagt ccactggtaa
     5641 cgctggcaat agatgccggc ttcgccacgg aaaaccacgc ccacatatag aggaaggcag
     5701 gcatgtctcc ataggcgttg cggatataga catattcgcc tcccgcgtag ggcatcatgg
     5761 cgccgagttc agcataggtc atagcgccaa aaagcgagag caggccgccg gccacccatg
     5821 caagatacac gagtcctgat gagccgacag cgcggaccat ttcatgcgga accaggaaga
     5881 ttccactgcc aataatagtg ccgacaacaa tggcagtagc gtgagaagcg ctaaggacac
     5941 gggggagttc gcgggtttgc ttggtctctt gcgttgcagg caacaaagaa aactccagct
     6001 tgggctatag cattttcaca gatagtcaga actatgccac gaaacttgag tcgccgcgac
     6061 caacagggag tcggcgacac tgcacatgca aatgcagtgt atagaatctg tgaaaatgcc
     6121 gcagtgtaat ggcgaaccgt gtcctgaaaa aacatggcgt ttcatgcgat tacgaatcgc
     6181 cacatctttt taaaccgtgg ggtacccggt ttcttctatg gcggataaat ttcatttctt
     6241 gctcttccaa aagcagagcg catgtataag actgtcttag cagtccagtc ttatagatgt
     6301 atagattttt ttcttgacga agcatagtcg gccagtggat agttggttcc acatgaaaaa
     6361 caggttggat gcatgtaatt ttggagggca aaggcaaaat ggaatccagg ttagttaaac
     6421 gttttgtatt gaatggcttt tttgctgtct tggttgtaac ttcgggtatg gcccgcgggc
     6481 agaccatctc cgggaccatt acgggtacgg tccaggattc caccggtgca gtcgtcccaa
     6541 atgcaaccat tacaatcaca aattccggcc agaatgtgac tgtttttaca gggaagacca
     6601 attctgctgg acagtacacg gcgccgtttc tgccggtggg caattacgct gtgagcgccg
     6661 aagcaccagg gttcaagaag gtagagcata caggactgaa gctcaatgtg aaccaggacc
     6721 ttactgtgga tgtggttctg gagcctggca gcgcgcagca gacggtcaat gtttccgcat
     6781 cggccctgca ggtagattta caaagcgcgc aagcacagac cgtcattaat ggtacgcaaa
     6841 tccggcaact tcctttgacg acgcggaact atgagcagct tgtagcgctg atgccgggag
     6901 tctcgaccgg actggcctct gaccagcttt atgttggcgc aagcaatcct gtggggactt
     6961 ccaatcaaat caatttttcg attaacggca gccgtccaac tcagaatacg tggaacattg
     7021 atggtgctga taatgtggac cgcggcgcga atctcacatt gcttgcttat ccgagtgtag
     7081 attccattgc agaattctca gtccaacgaa gcaattacaa tgctgagttt ggccgcagct
     7141 ccagcggtca gattaacgtc atcacaaatg cgggcacgga tcagtttcac ggagacgtat
     7201 atgaattttt ccgcaacgat gtactcgccg ccaacacata tctgaataac cggaaacagc
     7261 tcaggattgc tccactgcga tataacgact tcggtgggac aattggcggc ccaatatgga
     7321 aagaccacac attcttcttc ttctccgaag aggtccggcg agtcattacg tacacaaatt
     7381 ttattgccag tgttccaagc cttgcagaac ttaaaggaga tctctcagcg gctgctaatc
     7441 ctacggcatc aggtaagcca ggtcctgggc ctgtttgtgt gcaatggagt gcgccaggcg
     7501 gaacttgcct gcaacagggc taccaggtca cgaacatatc tccggcagcc caggcgtata
     7561 tcaaagacat cttcagcaaa cttcctgttc ccgacaatcc aaactgcacg ggtgggtgta
     7621 ctttgacttc cagcggtcgg aatatcttca acatgcgtca ggaaatcgtg cgcattgacc
     7681 aggtgttcag ccctaaattc agaatatttg gacgttttga aaatgacaca atcccaacca
     7741 cagaaccagg cggcttgttt attggcagtc cgttacccgg agttgccact tcttctacca
     7801 attctcctgg gcgcattttt actatccacg ccacgaatgc tatttcacct actttaatca
     7861 acgatgccgg atacaactat tctcatggcg gcattctgag cacgccaatc ggcctcacgg
     7921 cgggcaagaa ttctcctgat atcgcttcgg ccgtgaagct tccttataca tcgagcttga
     7981 acgtcgcgcc tgtacttgga tttacttact tcagcaatgt ttcagaccgt ggacaatatc
     8041 gtgatttcaa tgacaacaac aatgctttcg accatctctc gaaagttgcc ggacggcact
     8101 cgttgaagtt tggcgtcagc tacaactggt atcagaagga tgaaaactca gcgagcggca
     8161 atcagggcaa ttttggtttt cccatcactg cgccgaatgg aaacaacagc gaataccagg
     8221 aatgggccaa cttccttacc ggaaatgcaa gctcgtttgg acaagcatct gtagattttc
     8281 gtgctgtggt ccggcagcag cagtgggaga tgtatgcaca ggacacattc cagatacggc
     8341 ccaatctgac cttgtcttat ggtttgcgct actctctctt tcgacagcca acagatgcaa
     8401 aagagcatct gacgaacttt gatccggcca cttttgaccc cgctggcgcg cctcccattg
     8461 atccgaatac gggcaagatt gccgggaccg gaacgccctt gaacggcatt attgtcaatg
     8521 gcaagagttc gccctttggc gatgcagtga accagcaaaa cgatcttgat tttgcgccgc
     8581 gcgtaggatt tgcctgggac ccaataggga aggggcagat gtctgttcgt ggcggatacg
     8641 gaatgtttta tgattcctcc gcggtcagcc gttacgaaat caacgtgttc accaacccgc
     8701 catttgtgaa cagcctgtct ctcggtccta cattgctgga caacccgagc ggagcagcga
     8761 tcgtatcaag ctatccttct ccgctggctg cactcggagt aaagtggcat actccttatt
     8821 cgcaggaatg gaacctggat ttgcagcagc agttgcctgc tcagtggctg ttcgatattg
     8881 gctactatgg tggcgttggc aaacatctgc ttggcgaagt ggacctgaat cagccgcttc
     8941 ctggcgccta tgtaactgca ctcgctcctt acggggtaaa aggaccgcca acgcggggta
     9001 aaacaacaca gcagttgaac tatatccgcc cgtacaaggg ttatgatgcg attaatacgc
     9061 agatcaccga gtacatgtcg aactacaacg gcctgcaggt ggcgttgcag aaacgcttcg
     9121 gcgcaagttc attactgaac gtgaactata cgtggtcaag ggcaatgacc aacgctttca
     9181 acgatacaac ggcagcgcag aacacttacg atcttaaagc agagtatggt cctgcgcggt
     9241 atgaccgcag aaatatcttt aatgccaact ttgtatatgg actgccattc ttccaaacac
     9301 agtctggtct cgtgggccat gtactgggcg gatgggagct tacaggaatt gcatacgctt
     9361 ataccggact tccctataca gtgactggag tgttgaatga tccggcggga cagggcgtgc
     9421 ttgacggaaa cagcgcggtc agtggacggc ctgacctggt aggcaatcct aatcaggcaa
     9481 atgccgtaag cggccccatt cacaacaaca atggcattca gccatggttc aatacgtcag
     9541 cctatgcgca ggtgtgtcca acagtgagcg gggtcacgac ctgcccaaca ggagcgaggc
     9601 cgggcaactc tcatccaggc agcgtgcgtg gaccgggatt gtggcggggt gatctttcgc
     9661 ttcataagaa cacaaagatc actgagactg tcaacttgca gttccgcgcc gaagcgtcga
     9721 acgtcttcaa ccacactaat cctgacggca tcagcacgtc atatacatca agcctgtttg
     9781 ggaaggtcac gtcatatcgt gatccaagaa ttttgcagtt ggctcttaag cttaacttct
     9841 aaccacttcc gtatttgcat tacttttgac ctccaaccgg cctgtgtaga aaacgcaggc
     9901 tggttttctt ttgcattgtc actggttaat ggattaattg tcaggagtac tcaaatcgaa
     9961 gatggcgggc cgacacggct gtagagccgg ggaagggcaa cattgcagac ctgtactact
    10021 tcgcgtgtgc ggcccttaat ccgctgcgta tgggttgaca ggcctcggga aaggcaaccg
    10081 gcgtgcttac tactatctga acagattcga tactttaatc ggtgaaatcg gtccgcgaag
    10141 acgagcgact ctttattatt gccaaacaaa aaaaagacca ggacgaatcc cggtctttcc
    10201 gttctggtcc gaattaagcg gcctttgccg acagcgcctt gaggcggacg gcaagacggc
    10261 tcttatagcg agacgcggtg ttgtcgtgca gaatgccttt ttgcacgctc ttgtcaatct
    10321 gggagacagt cttgcggaac tgctcgtggg ccgccgcgac atcgcccttg gcaagagctt
    10381 cgcggagaga gcggatggca ccgcggacac ggctcttgtt ggcgcggttg accgcggtct
    10441 ttctctctgt ctgacgggcg cgtttgagcg ccgaaacatg gttcgccatg atgcttcct
//
LOCUS       DASRBV010000012         7325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_507296, whole genome shotgun sequence.
ACCESSION   DASRBV010000012 DASRBV010000000
VERSION     DASRBV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507296"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFA02_00315"
     CDS             <1..738
                     /locus_tag="VFA02_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08317.1"
                     /translation="ASVNNLLAQAYVGQSQPQKALEAFHRAVALDPDNEKLYAFIADA
                     CTDHKNFDLGLQVVNEGLKNLPRSARLHYERAMFLAQTGNDVDSQAEFAEAAKLAPET
                     DIAYLALAQKNLFAGNISEAIRVAREAIRAGHNDPVLLDLLGEVLIHAGATPGQPEFA
                     EAQTLLEHAVAQKPDYSTAQIALGRLYLMEDRPADAVTHLEIGRRLEPENRAVYTSLA
                     DAYRKSGRKDKAREMMDVLGKLMANQQ"
     gene            complement(811..2727)
                     /locus_tag="VFA02_00320"
     CDS             complement(811..2727)
                     /locus_tag="VFA02_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU08318.1"
                     /translation="MRTTSVFFTALVLAAIGFVPGSVAQSDRVTPDMVQTGSDIPAKW
                     EEPHPGYDYERRDVMIPMRDGVRLHTVILVPKGAKGLPMLLERTPYNAANFSSDKPKM
                     RDAVWPAQREWVDDGYIIVDQDIRGKYGSEGDYVMTRPPMGPLNPTKTDDTSDAWDTI
                     DWLVKNVPESNGRVGMIGSSYDGWTVAMALLGPHPALKVAAPESPMIDGWMGDDWFHY
                     GAFRQVNLDYFTEQSSERGPGSPVPRPGDDDYKTFLDAGTAGHWAEEHGFKQLPWWNR
                     LSAHPAYDAFWQLQALDKLLAARPASIPTLWLQGLWDQEDIYGAVHAWEALKAAGHAG
                     NNHLVMGPWYHSQVNRSAETLGPLKWKGDTAADFRRDVLVPFFDAYLKDRKLPEPLPP
                     ALIYNPSENHWDKFADWPVVDEKHLTPLYFESNFGLGPEKPRGGEDSYVSDPAKPVPF
                     LTPPIEPGGGSRWRTWLVEDQRSVGTRTDVLSYETPVLQEPVRVQGAPFADLFVKTTG
                     TDADFVVKIIDVYPPTYAQQPELAGYELPVSLDIFRGRYRKSFAEPEAIPAGTVQEYR
                     FRLPTVNYIFKPGHRIMVQVQSSLFPLYDRNPQTFVPNIFFARPEDFRAATMTVEHGP
                     QGASAVLLPIVEEK"
     gene            complement(3012..3953)
                     /locus_tag="VFA02_00325"
     CDS             complement(3012..3953)
                     /locus_tag="VFA02_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZU08319.1"
                     /translation="MHVHVAPTGKMQRVLRFSLAATLIYVILTFVAGLRAHSLALISE
                     AGHNISDALALLLSFVAVYFQTRPATDEKTFGYQRAGVLAGFVNALSLIVIAVFIAIE
                     AVHRLSEPVQVQARLMMIVAAAGVLMNGVIAFLLSRVNTDVNIRSAFIHMLGDTLSTA
                     AVIVGGATILWTGHSWIDPVLSLGIAALIIWSSVAIVRETLNILLEGTPRGISLAEIR
                     RELQKIHGVEDVHDLHVWSLGSQTHALASHVTIADIPPSESGRILDQIKGVLHERFHI
                     DHTTIQFENAGCEVAHGCVMAVEEVHFHAHHGHSHSH"
     gene            complement(3968..4450)
                     /locus_tag="VFA02_00330"
     CDS             complement(3968..4450)
                     /locus_tag="VFA02_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08320.1"
                     /translation="MNSFSLRDPDVIGTLTVAALCLGGVAAWFMLRKRPNPEDIERGR
                     RSYLVSNGRIIDGTLLDISDLSPEESGRPTGMQLILYKYEIGGVEYECSQDVTSLRDL
                     VNIYECRLGFPCSVRYDTHKPENSIVVAETWSGLRDTASSVPVRRSMPKPMRSWRPTR
                     "
     gene            4702..5187
                     /locus_tag="VFA02_00335"
     CDS             4702..5187
                     /locus_tag="VFA02_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HZU08321.1"
                     /translation="MQCPSCHNEVSPQSTFCGFCGSPVSAAAAAPAGGGSATVPVATP
                     AASGLSENAAAAISYVTIIPAIIFLVMEPYNKMRLVKFHAIQCIALHVAWVAVWVAAT
                     ILQIVLHFIPFIGLLFVLVDLGIGVVFFIAWLMAILKASKGEYFKLPVIGEFAAKQAN
                     A"
     gene            5355..6011
                     /locus_tag="VFA02_00340"
     CDS             5355..6011
                     /locus_tag="VFA02_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08322.1"
                     /translation="MILSIRSLRVLFALSVCFFAFTSLHAQVQATAPVTILPQYEERW
                     DIYGGFAASHFNPGAGRNVQAIWLNGWNGDATVWVKQVFGVEFSARGFYGTIIVPPNN
                     LGISKSNMSEHLFLVGPNFRMLRRDNYAAGFHVLMGAANGSFDQGFKGTGVQPYQLTI
                     YNNKVAYGMAVGGWLDYNLNPRWAVRVTTDWQPTHYGYMTQNEFAGTVGVVYKIGHLK
                     "
     gene            6051..6926
                     /locus_tag="VFA02_00345"
     CDS             6051..6926
                     /locus_tag="VFA02_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZU08323.1"
                     /translation="MLNAWIVAAAVLALAPIFVFGFAGQRLQAAARHLSPGARLLSPA
                     ACVLPYLLVAVPLHLFHIQWMLLYIELPVAVALLLALAARFDPEQKGHWLDFLVLFSL
                     GLAVDLRWFEAAWPHTLAAMNKVLLLDAGLYGFHIIRQLDGVGFDLRWRWQDWKTGLR
                     EFLIYAPLAILLGLAIGFLHLHTHVQRPWTVPFVWIFTYVLIALPEEIFFRGWMQNLF
                     ERKLGRTTALFLTAVLFGLSHFNKRAAHFNWRYVVLAAIAGVFYGRAWRENRRVAASA
                     ITHASVDTIWGALLR"
     gene            complement(6915..>7325)
                     /locus_tag="VFA02_00350"
     CDS             complement(6915..>7325)
                     /locus_tag="VFA02_00350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HZU08324.1"
                     /translation="RAGTLHPLFGRKIPLAVRIGGYFLWSFLQEFILQSYVLLRLLRL
                     APGRKAAVAMAAAMFAIAHLPNPVLTGLTFIWGAIACPLYLRYRNLYVLGMAHAILGL
                     CVAITVPDSIHHHMRVGIGYITYPKDMHVPVSPQ"
BASE COUNT         1559 a         2176 c         1987 g         1603 t
ORIGIN      
        1 gcaagcgtga acaatctgct ggcccaggcg tatgtcggac agtcgcagcc gcaaaaggcc
       61 ctggaagcct ttcatcgcgc cgtggcactg gacccggaca acgagaagct gtatgccttc
      121 attgccgacg cctgcaccga ccataagaat ttcgacctcg gcctgcaagt ggtgaatgag
      181 ggactgaaaa accttccccg ttcggcgcgc ctgcattatg agagggcgat gtttctggca
      241 cagaccggaa atgatgtaga ttcacaggcg gaattcgcag aggcagcaaa gctcgctccg
      301 gaaacagata ttgcctatct cgcgctggcg cagaaaaatc tttttgccgg caacatttcc
      361 gaagccattc gcgtggcgcg cgaagccatc cgcgccgggc ataacgatcc ggtgctgctt
      421 gatctgttag gcgaggtgct gatccatgct ggagctactc caggccagcc tgaatttgct
      481 gaagcgcaaa cacttctgga acacgcagtg gcgcagaaac cggactactc caccgcgcag
      541 atcgccttag gcaggctata tctcatggaa gaccgtcccg cagatgcggt gacccatctg
      601 gaaattggac ggcgtctgga gccggaaaac cgggcagtgt acacgagtct ggcagacgcc
      661 taccgaaaat cgggccggaa agacaaggcg cgcgaaatga tggatgtcct gggaaaatta
      721 atggcgaatc agcagtaaag ccgcagcgga taccccatcc tgtcgcccct gtctgcgcga
      781 cagggatggg ataccacacc actgagaggc tcatttctct tccacaattg gcagcagtac
      841 tgcacttgcg ccctgcgggc catgctccac cgtcatggta gcggcgcgaa aatcctccgg
      901 tctggcaaag aagatgttag gcacaaaggt ctgcgggttg cggtcataga gcggaaacaa
      961 tgaagactgc acctgcacca tgatgcggtg tccgggcttg aagatgtagt tcaccgtcgg
     1021 cagcctgaag cgatattcct gcaccgttcc cgccggaatc gcctcgggct cggcaaagct
     1081 cttcctgtag cgtccgcgga aaatgtccag actgacaggt aactcgtaac ctgccagttc
     1141 tggctgttgc gcgtaggtag gtggatacac gtcaatgatc ttcacgacaa agtctgcgtc
     1201 cgtcccggtc gtctttacaa aaagatcggc aaaaggcgcg ccctgcaccc tgaccggctc
     1261 ttgcagcaca ggcgtttcat aactcagcac gtctgttcgc gttcccacgc tcctctggtc
     1321 ttcgaccaac caggtcctcc agcgcgagcc tccgccgggt tctatcggtg gtgtcaggaa
     1381 cggtacaggc tttgccggat ccgaaacgta gctgtcttca ccgcctcgcg gtttctccgg
     1441 ccccaaaccg aaattgcttt cgaaatacaa gggcgtcaaa tgcttttcat ccactacggg
     1501 ccagtcggca aacttgtccc agtggttctc tgaggggttg tagatcagcg ccggaggcaa
     1561 gggctcgggt agcttccggt ccttcagata agcatcgaag aaaggaacca gcacatcgcg
     1621 ccgaaagtcg gctgcagtgt cccctttcca cttcaacgga cccagcgtct cagccgatcg
     1681 gttcacctgg ctgtgatacc acggccccat caccaggtgg ttgtttcccg cgtgcccggc
     1741 cgctttcagg gcctcccagg catgcaccgc cccatatata tcttcctggt cccacagccc
     1801 ttgcagccac agagtgggaa tgctcgccgg cctggccgcc agcagcttgt ccagcgcctg
     1861 caattgccag aatgcgtcat acgccggatg cgccgagagc cggttccacc atggcaactg
     1921 tttgaagccg tgctcctcag cccagtggcc tgccgttcca gcgtccagaa aggttttgta
     1981 atcgtcgtcg ccagggcgcg gcacggggct gcctgggcct cgctcgctcg attgctccgt
     2041 gaaataatcc agattcactt gacgaaacgc gccatagtgg aaccagtcat cacccatcca
     2101 gccatcaatc atcgggcttt ccggcgcggc caccttcagc gccggatgcg gccccaaaag
     2161 cgccatggcc actgtccatc cgtcatacga tgagccgatc atgcctactc gtccgttcga
     2221 ttccggcaca ttctttacca accagtcaat cgtgtcccag gcatcgctcg tgtcatcggt
     2281 cttcgttgga ttcagtggcc ccatcggagg ccgcgtcatc acgtaatcgc cctccgagcc
     2341 atatttgccg cgaatgtcct ggtcaacgat gatgtagccg tcatctaccc actcacgttg
     2401 cgccggccac accgcgtcgc gcatttttgg cttgtctgag gagaagttgg cggcgttgta
     2461 aggcgtgcgc tccagcagca tgggcagtcc ttttgctccc ttgggcacca ggatgaccgt
     2521 gtgcagcctg accccatcgc gcatgggaat catcacatcg cgccgctcat agtcgtagcc
     2581 cggatgtggt tcttcccact tcgcaggaat gtcgctgccc gtctggacca tgtccggagt
     2641 cacccggtcc gactgggcaa cagaccccgg tacaaaacca atcgccgcga ggaccagagc
     2701 agtgaaaaaa acagacgttg tacgcatggc tcttatcttt tcaacagccc agcaagcatg
     2761 acaaccatat ttcttctttg cgttgacatt ctgccaggtg cctgccgccg ttgcgcgaaa
     2821 gggcggaagg ccaggaacca ctcccactct gattcgccta agaggcatgt actatagcat
     2881 tattcgtata tagacagtcc gtcattctga cgaccgaagg gaggaggaat ctcagcgatt
     2941 cagggtttac ccgatactgc taagatcttc ccaaccttgg aatgatgatc agcttttccg
     3001 tggattacat tctaatgact gtggctgtgg ccgtgatgcg cgtgaaagtg cacttcttca
     3061 accgccatca cgcaaccatg cgcaacttca catcctgcat tttcaaactg gatggttgtg
     3121 tggtctatgt gaaaacgttc gtgcagcaca cccttgatct ggtcgaggat gcggccgctt
     3181 tcagacggtg gaatgtccgc aatcgtcacg tggctggcca gagcgtgcgt ctgcgagccg
     3241 aggctccata catgcaggtc atgcacatct tccacgccat ggatcttctg caattccctg
     3301 cgaatctcag ccagcgagat accgcgcggc gtcccttcca gcagaatatt cagtgtctcg
     3361 cgcacaatgg caaccgatga ccagatgatc aacgccgcaa tgcccagaga caataccggg
     3421 tctatccatg aatgccctgt ccacagaatc gtcgcaccac ccacgatcac tgccgccgtt
     3481 gagagcgtgt ccccaagcat gtgaatgaat gcgctgcgga tgttgacatc ggtgtttacg
     3541 cgcgacagca ggaaggcaat caccccgttc atcaggacac ccgccgcggc cacaatcatc
     3601 atcagacgcg cctgcacttg caccggctca ctcaggcgat ggacagcctc gatggcaatg
     3661 aataccgcga tcacaatcag cgagagcgca ttgacaaacc ccgccagcac gcccgcgcgc
     3721 tgatagccaa aggtcttctc gtccgtcgct ggacgcgtct gaaaatacac agccacaaag
     3781 gaaagcagca gcgccagcgc gtcggaaatg ttgtgtccgg cctcggagat gagcgccagc
     3841 gaatgggcgc gcagtcctgc gacaaaggtg aggatcacgt aaataagcgt cgccgccagc
     3901 gaaaagcgca ggacacgctg cattttgccc gttggcgcga cgtgtacgtg catatatctt
     3961 ctttagttta tcgtgttgga cgccatgagc gcatcggctt gggcatcgag cgtcgcaccg
     4021 gaacggaact cgctgtatcg cgcagtccac tccacgtctc ggcaaccacg atgctgtttt
     4081 ccggcttgtg cgtgtcgtag cggaccgagc aggggaagcc caggcggcat tcgtaaatat
     4141 tcaccaggtc gcgcagcgaa gttacgtcct gcgaacactc atactcgacc ccgccaattt
     4201 catatttgta aagaatcagc tgcatcccgg tgggacgccc gctttcttcc gggcttaagt
     4261 ctgagatatc gagcagcgtc ccatcaataa tgcggccgtt actgaccaga taagagcgcc
     4321 tgcccctttc gatgtcctca ggattgggcc gttttcgcag catgaaccag gccgccacgc
     4381 cgcccaggca aagcgcagca accgtaagcg tgccgataac gtcgggatcg cgcagggaga
     4441 aactattcat ccgggtgctc tcgtgcctga tagcagaata ccccagatgt aagcgcggat
     4501 tctgcacagg atcgggccaa caccccaatt tttcataaca aagcctgaaa cggtctccag
     4561 agacgcagaa ctatccctcg agcgcagtga gacagcaaaa ttttgcgtgc ccggaccatc
     4621 ctgacaggcg tatgctaagg gcgttccgag gaaggtcccc ccgctcgctc cttagaaatt
     4681 ctgaattgag gagaaaactc catgcaatgt ccttcttgcc acaacgaggt cagtccccag
     4741 tccacattct gcggtttttg cgggtcaccc gtcagcgctg cagccgctgc tcccgccgga
     4801 ggcggctcgg ctacagtccc tgtagcgacg cctgcggcca gcggactctc agaaaacgcc
     4861 gctgcagcca tttcctatgt caccatcatt ccagccatca tctttctggt gatggaaccc
     4921 tacaacaaga tgcgcctggt gaagttccac gccatccagt gcatagcgtt gcatgtggca
     4981 tgggtggcgg tatgggtggc agctaccatt ctgcagatcg tcctccactt catcccgttc
     5041 attggtctgc tctttgtgct ggtggacctg ggaatcggcg tagtgttctt tattgcgtgg
     5101 ctcatggcaa ttctgaaggc cagcaaagga gaatacttca agctcccggt gattggtgag
     5161 ttcgcggcga aacaggccaa tgcctgaaga accggtcacg gtcagtgtac gccaggcgct
     5221 tttggccaac tcgcttgcgc cataagcgtg cgctgaccgc ccgtagctga tctgctttca
     5281 tctctggcct ggctgcgtcc aaccgtgtat cgtgaatatt tgtggtattc ccgaactgag
     5341 gttgaagaaa tagcgtgatt ttgagcatcc gttcgctgcg cgtcctgttt gcgctttctg
     5401 tttgtttttt tgctttcaca tctttgcatg cgcaggtgca ggccactgct ccggtgacca
     5461 tccttccgca atatgaagag cgctgggaca tctacggcgg tttcgccgca tcgcacttta
     5521 accccggcgc aggcagaaac gtgcaggcca tctggctcaa cggctggaac ggcgacgcca
     5581 ccgtatgggt caaacaggta tttggtgtgg aattctcagc tcgcggcttc tacggcacca
     5641 tcattgttcc gccgaataat ctgggcatct caaaatccaa tatgtcggaa caccttttcc
     5701 tggtcggccc caattttcgt atgctccgga gagacaacta tgcagcgggc ttccacgtgc
     5761 tgatgggcgc tgccaatggc tcattcgatc agggattcaa aggcacgggc gtccagccgt
     5821 atcagttgac gatctacaac aataaagtgg cttatggcat ggccgtcggc ggatggctgg
     5881 actacaacct caaccccaga tgggccgtgc gcgttacgac ggattggcaa cccacccact
     5941 acggctacat gacccagaat gagtttgccg ggacggtggg cgtggtctat aaaataggac
     6001 atttgaaata ggcttcatct gtaaacagca gctccttcgc ttacattttt atgttgaacg
     6061 catggatcgt ggccgcggcc gtcctcgcgc tcgctccgat tttcgtcttt ggctttgccg
     6121 gacaaagact gcaagcggcc gcgcggcatc tgtctccggg tgctcggctc ctgtcgcctg
     6181 ctgcctgtgt ccttccttat ctgctggtgg ccgtgcccct gcatctgttc cacatccagt
     6241 ggatgcttct gtatattgag cttcctgtgg ccgtggcgct gttgctggcc ctggccgcca
     6301 ggtttgatcc ggaacaaaag ggccattggc tggattttct ggtcctcttc tctctcggac
     6361 tcgccgttga cctgcgctgg tttgaagctg cctggccaca cacactggct gccatgaata
     6421 aagtcctgct gctcgatgcg ggactctatg ggttccacat catccggcaa ttggatggcg
     6481 tcggttttga cctgcgctgg agatggcagg attggaagac cggcctgcgc gagttcctta
     6541 tctatgcgcc cctggcaatt cttctgggac tggccatcgg gttcctgcat ctgcatacgc
     6601 atgtgcagcg cccgtggacc gtgccgtttg tctggatatt cacatatgtc ctgatcgccc
     6661 ttcccgaaga aatctttttc cgtggatgga tgcaaaacct ctttgaacga aagctggggc
     6721 gaacaaccgc actctttctc acagcagtcc ttttcggcct ctcccacttt aataaacgcg
     6781 cggcgcactt caattggcga tatgttgtgc tggcggcaat tgccggggtc ttttatgggc
     6841 gggcatggcg cgaaaaccgc agagtggccg cttccgccat cactcacgcg tcagtagata
     6901 caatctgggg cgcattattg cggtgaaacc ggcacatgca tgtctttggg ataagtaata
     6961 tagccgatgc ccacccgcat atggtgatgg atagaatcag gcactgtaat cgctacgcaa
     7021 agtccaagaa tggcgtgggc cattcccagc acatataaat tgcggtatcg cagataaagg
     7081 ggacatgcaa tggcccccca aatgaacgtc agtcccgtca gcactggatt cggcagatgc
     7141 gcgatggcaa acatcgcagc ggccatcgcc acagcagctt tacggcccgg cgccaggcgc
     7201 aacaaccgga gcagcacata ggattgcagg ataaactcct gtagaaacga ccagaggaag
     7261 tatccgccaa tacgcacggc cagcggtatc tttcgtccaa agagcggatg cagcgtgccc
     7321 gcgcg
//
LOCUS       DASRBV010000013         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_51224,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000013 DASRBV010000000
VERSION     DASRBV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51224"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFA02_00355"
     CDS             <1..280
                     /locus_tag="VFA02_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU08325.1"
                     /translation="TRDPAVPVENSLDLYRTLERAHVPVEMHLYDYANHGCGLCGDIP
                     ELSTWPLLLRNWLIHGGWLPKDAPPLPPIQVRYPSWPEGLEGPGKITR"
     gene            411..1385
                     /locus_tag="VFA02_00360"
     CDS             411..1385
                     /locus_tag="VFA02_00360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZU08326.1"
                     /translation="MSQTTYTVQPEAAGQRLDAWLTTQLENVSRSRVQMLLSQNKVLV
                     DGKAAKASYKLRGSEAIEVLGDPAPPPLRAIAEKIPLEIVYEDDDLSVVNKPAGMMVH
                     AGSGATEDARNRGTLVNALLHHYRQLSSAGGELRPGIVHRLDKQTSGLIIVARNDTTH
                     LKLAEMFSRRQIRKTYIALVHGHLKQDTGTINAAISRDLLRRTRMTTRRTTGGRTAIS
                     HYEVMERMESRFGKFTLVRVRIETGRTHQIRVHMAAIGHPIVGDTLYGAPARIAVPSR
                     GKEAESVALHRNFLHAAELAFAHPRTGKLLELSAPIPEELKKFLAQLG"
     gene            complement(1414..1875)
                     /locus_tag="VFA02_00365"
     CDS             complement(1414..1875)
                     /locus_tag="VFA02_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08327.1"
                     /translation="MQRKTVFLAMMGMLLASCLSAKAQELGYWRATSNTAQSITGDIA
                     FSDAKLLMNFSTFPIVQARELSSAEVSAVFDADSNSPAKGHLYKIDIPAQKKFLHKNT
                     LCGQESAQWMVTYAQGKSLSVAFFSGQKPPVFTLDAIQNSTSLCGTFFYTR"
     gene            complement(2053..>2997)
                     /locus_tag="VFA02_00370"
     CDS             complement(2053..>2997)
                     /locus_tag="VFA02_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU08328.1"
                     /translation="YLADHKVKVPVVVMAIENYGRVYRLLEAHVPVTIEMDVETKFTG
                     DHEHGYDTIAEIPGTDPKLKDEIVMVGGHLDSWIAGTGATDNGAGSVVAMEVMRILKA
                     LDVHPRRTIRIALWSGEEEGLFGSRGYVKQHFGYAAPLNTPDQQNLPEFLRKTGPLEI
                     KPEQKLVSAYFNVDNGSGKIRGVYLQENAAVAPIFAQWIAPLKDLGVTTLSMRNTGGT
                     DHLSFDAVGIPGFQFIQDDLDYESRTHHSNMDTYERLQAADLKQIATVEAIFVYNAAM
                     RDQMIPRKPLPHPELRDKQNAPLPNLFPGAVTPPEEKK"
BASE COUNT          648 a          843 c          867 g          639 t
ORIGIN      
        1 aacccgagat cccgcggtcc cggtagaaaa cagccttgac ctgtaccgca cgctggagcg
       61 ggcgcatgtg cctgtcgaga tgcacctgta tgactacgcg aaccacgggt gcggcctgtg
      121 cggcgatatt ccggagcttt ctacctggcc tctgctgctg cgaaactggc tgattcatgg
      181 cggatggttg ccgaaggacg ctccgccgct gccgccgatt caggtgcgtt atccatcatg
      241 gcctgaagga ctggaaggcc cgggtaaaat cacccgctaa ggcattttca cagattctgt
      301 acacaccatt tgcactttgc agtgccgccg actcgctgtt ggccgcggcg gctgtagttt
      361 cggggcatgg ttcagattat ctgtgaaaat gctataagtt tgttattttc atgtcgcaaa
      421 ctacatacac cgtccagccc gaagcagcag gccagcgcct tgacgcatgg cttacaacgc
      481 agcttgagaa cgtaagccgg tcgcgcgtgc agatgctgct ctcacagaac aaagttcttg
      541 tagacgggaa ggcggccaaa gcatcttaca agctacgtgg cagtgaggcc atcgaagtgc
      601 ttggagatcc tgcgcctcct ccgctgcggg ccatcgcaga aaagattccg ctcgaaattg
      661 tttacgaaga tgacgatctt tccgtcgtaa acaagccagc gggaatgatg gtccatgcag
      721 ggtcaggggc gaccgaggac gcgcgcaatc gcggcacgct ggtgaatgct ttgctgcacc
      781 attatcgcca gctttcgtca gcgggaggcg agctgcggcc aggcatcgtc catcgtctgg
      841 acaagcaaac cagcggactc atcatcgtcg cccggaatga cactacgcat ctcaagctgg
      901 ctgagatgtt ctcacgccga cagattcgca agacatatat cgcgcttgtg catggccatc
      961 tgaaacagga cacgggcacg atcaacgctg cgatcagccg cgacctcctg cgccgcacgc
     1021 gcatgaccac gcggcggacg accggaggac gcacagccat ctcgcattat gaggtgatgg
     1081 aacgcatgga atcgcgtttt ggaaaattca ctctggtgcg cgtgcgcatt gaaacaggcc
     1141 gcacccacca aatccgggtg cacatggccg ctatcggaca ccccattgtg ggcgatacgc
     1201 tctatggagc accggcgcgg attgcggtgc catcaagagg caaggaggcc gaatctgtcg
     1261 cgctccatcg caatttcctt catgccgcgg aactggcctt cgcccatcca cgcacaggaa
     1321 aactgctgga actcagcgcc ccaatcccgg aagagctgaa gaaatttctg gcgcagttgg
     1381 ggtaagctgt tcagcggagc acggtcctga gcttcagcga gtatagaaaa acgtcccgca
     1441 cagactggtc gagttctgaa tcgcatccag ggtaaacacg ggaggcttct gcccggagaa
     1501 aaatgcaacg gagagcgatt ttccctgggc atatgtgacc atccactggg ccgactcctg
     1561 gccgcagagg gtgtttttgt gcaggaattt cttttgcgca ggaatatcaa tcttgtacag
     1621 atggcccttg gccggagagt tgctgtccgc atcaaacact gcgcttactt ctgccgaaga
     1681 aagctcgcgc gcctgcacaa tgggaaaagt agagaaattc atcagcaact ttgcatcaga
     1741 gaatgcaatg tcgccggtaa tggactgggc cgtattgctt gttgcccgcc agtacccaag
     1801 ttcctgtgct ttggctgaaa gacaggatgc caggagcatc cccatcattg cgaggaacac
     1861 tgtttttcgc tgcattcttt aaggatgaca ctcctttccg ttcccggcaa tcagcaggga
     1921 gccaggtaca aggcagagcc tgtcgcggtc gcccgaaaaa tcgaaaaata agggagatat
     1981 tgctggtgtc gccggctgag tcatggactg acaggcgcag cgatgggccg cgcctttcag
     2041 taaccgcact ggctactttt tctcttccgg cggagttacg gcaccgggga aaagattcgg
     2101 gagcggcgca ttctgtttgt cgcgcagctc cggatgcggc agcggcttgc gcggaatcat
     2161 ctgatcgcgc atcgctgcgt tatacacaaa gatggcctcg acagtcgcaa tctgcttcag
     2221 gtcggcggcc tgcaggcgct cgtatgtgtc catattcgag tggtgtgtgc ggctttcgta
     2281 atccaggtcg tcctgaatga actggaagcc tgggataccg acagcatcga acgaaagatg
     2341 gtcggtaccg ccggtgttgc gcatggaaag cgttgttacg ccgaggtctt tgagcggggc
     2401 gatccactgc gcgaagatgg gcgcgaccgc agcgttttcc tgcaaataca caccgcgaat
     2461 tttgcctgag ccgttgtcca cattgaaata agcagaaacg agcttctgct cgggcttgat
     2521 ttccagcggc ccggtcttgc gcaggaactc cggcagattc tgctggtccg gggtgttcag
     2581 cggagcggca tagccaaaat gctgcttcac gtatccgcgc gagccgaaga ggccctcttc
     2641 ttcaccgctc cacagagcga tgcggatggt ccggcgggga tggacatcca acgccttcag
     2701 aatgcgcatg acttccatgg caaccacgga accggcgccg ttgtcggttg cgcccgtgcc
     2761 tgcaatccag ctgtccagat ggccgccgac catcacaatt tcatctttca gctttgggtc
     2821 tgtgccgggg atctctgcaa tggtgtcata gccgtgctca tggtctccgg tgaacttcgt
     2881 ctccacatcc atctcgatgg tcacagggac atgcgcttcg agcaggcggt atacgcgacc
     2941 gtaattctca atggccatca cgacgacggg gactttcact ttgtggtctg ccagata
//
LOCUS       DASRBV010000014        12304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_659457, whole genome shotgun sequence.
ACCESSION   DASRBV010000014 DASRBV010000000
VERSION     DASRBV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659457"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFA02_00375"
     CDS             complement(<1..1037)
                     /locus_tag="VFA02_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU08329.1"
                     /translation="METTEQLSARTLRRVSCRLLPFLFALYVFAYLDRVNVGYAALFI
                     RRELGFSDTVYGTGAGLFFLSYALFEVPSNLVLLRLGPRRWIGTLMVVWGVISASMPL
                     VRTPSEFYLLRFLLGAAEAGFFPGIILYLTYWFPQKQRARAVALFMAATAIAGVIGAP
                     LSGLLLRLDGVAGVAGWKWLFVGEGLPSVLLGALVFLVLTDSPERAAWLGPDERTWLT
                     ETLAAEQVSISASHSVHLRDVWRNAVLWQLAFIYFAISVALYSLSLWLPVVLKQLSRA
                     SDLRVILLTAIPYLLAAIAMLLIARSSDEKQERRWHLFASLTGAAAGFGLSVVLQSPW
                     VAMSALFLAAAG"
     gene            complement(1046..2200)
                     /locus_tag="VFA02_00380"
     CDS             complement(1046..2200)
                     /locus_tag="VFA02_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HZU08330.1"
                     /translation="MGLLAAPIWMLAQSSQIAATPPMGWNSWNHFAERVTDADVRAAA
                     DALVSSGMRDAGYIYVNIDDTWEGQRDANGVIHTNSKFPDMKALADYVHSKGLKLGIY
                     SSPGPKTCAGYEGSYGHEEQDAKTYAAWGIDYLKYDLCSFIEIMKKETGDPHSEKAFE
                     MQKAAYEKMHKALEATGRPIVFSLCQYGWNDVWKWGPEVGGNLWRTTGDISDRYSRMA
                     EIGFSQAGLSKYAGPGHWNDPDMLEVGNGGMKTEEYRTHMSLWAILAAPLLAGNDLSK
                     MSKETLDILTNKEVIAVDQDKLGKQGDRVWEEGPKEIWAKPLSGGAKAVGLFNRADSP
                     RNITLQLKDVGFSSNAKLRDLWKHEDVTARNGSYSVLVPGHGVVMLKISQ"
     gene            2355..3401
                     /locus_tag="VFA02_00385"
     CDS             2355..3401
                     /locus_tag="VFA02_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HZU08331.1"
                     /translation="MKLLLKMLALCVATATVYAQATPRDSLLVLSKVDRTLSIVDPVS
                     LKVMARIPAGNDPHEVAVSADGKLAFISNYGYGAYNTITVVDLAARQGLNTIDLGPLH
                     GPHGLAFAEGKLWFTAEVAKAIARYDPSTNKVDWILGLGQNRTHMIYVSQDASRIITT
                     NVNSGTVSVIDRVAVHPQGPVAGAQTHRPNYTQPHGPAFDWNETFIPVGKGPEGFAVS
                     PDGKEVWVGNGLDGTVSIIDIDAKKVIETISANIPGINRVKFTPDGRLVFISSLRSPN
                     VTVFDAATRKEIKRIPVGHASEGILVQPDGARVFISCSPDDYVAVIDTKSLRTTGRIE
                     AGHEPDGLAWAVQH"
     gene            complement(3330..4670)
                     /locus_tag="VFA02_00390"
     CDS             complement(3330..4670)
                     /locus_tag="VFA02_00390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU08332.1"
                     /translation="MGLEKYDVAVLGAGIAGLTGARILATQGKRVALIEARSRIGGRI
                     FSLPQQRSDLPVELGAEFIHGRPVEVWQLIEEAGLHAYEMDGTMLCFDENSLHECDEG
                     PGGFDLMRRLIDNGPDQSFAEWLAQQELPAPIVESAIAFVEGFNAADAARIGTAALAK
                     QQAAEDAIQGHLAFRLREGYSALPAFLLKQFQSHGGEVFLSAPVISVAWRRGNVRIHA
                     MQQYQERVFEADQCIVTVPLGVLQSGSIHFAPSPSKTFHAVSQMAMGSAKRITFLFRE
                     RFWQMKFPNAHFLFSQQDLMRVWWMPSPDLTPMLTGWIGGPKSLDASLFPDTDFAALG
                     LRTLAKIFSVPEEHLRELLVSWHTHDWQRDPFSLGAYSYAPKGALEASEVMSIPVEDT
                     LFFAGEHTDTTGHWGTVHGALRSGQRAARQVLMLDGPGKPIGFMSGFNTARGPQ"
     gene            complement(4683..5438)
                     /locus_tag="VFA02_00395"
     CDS             complement(4683..5438)
                     /locus_tag="VFA02_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZU08333.1"
                     /translation="MSEPNHNHETHLTMQVVNHIRRLIENGTLRPGDKIPPEREFAQK
                     LKISRASLRAGIGHLAAMGVLNVRHGVGTFVADGPPALGGVSLDILRALHGYTTAQMF
                     EARRILESHLAALAAERGREEHFTALAEEVTEMYATFHEPQEYLIHDMRFHRTIAEAS
                     GNPILAVLMETITAALYDERRKTVERSRDLKQSAEVHRELYRAIRSRNAREAREVMER
                     HLHMAESAQVNEQALAKEEKRPAAKKKSARASA"
     gene            5572..6966
                     /gene="uxaC"
                     /locus_tag="VFA02_00400"
     CDS             5572..6966
                     /gene="uxaC"
                     /locus_tag="VFA02_00400"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HZU08334.1"
                     /translation="MAILHEDRLFPADPETRKIARRLYATVRDLPIISPHGHTEAVWF
                     AKNEPFPDPAQLFILPDHYVYRMLYSQGVSMHELGIRQPKPPDPRKIWRIFAENYYLF
                     RGTPTRLWLDYEFETLFGLKDRLSAKNADHYYDVIADKLQTPQFLPRALYERFRIEVL
                     ATTDSPLDTLVHHRALRESGWKGRIVPTFRPDPVVDPAFAGFRENIAKLGELTGEDTS
                     KWSGYLNALRKSRERFKQLGCTATDHGHPTANTANMSTAEASALFDVVLSGKSSPPQQ
                     ETFRAQMLTEMARMSVEDGLVMQIHPGSFRNHNQKVYEQFGRDAGADIPTATDYVHAL
                     KPLLDLFGNEPSLTIILFTLDESAYSRELAPLAGHYPCLKLGPPWWFFDSPEGMLRFR
                     ELATETAGFYNTVGFNDDTRAFLSIPSRHDVARRMDCAFLARLVAEHRLDEDEAAEVA
                     QDLTVNLVRKAYRL"
     gene            7035..8348
                     /locus_tag="VFA02_00405"
     CDS             7035..8348
                     /locus_tag="VFA02_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU08335.1"
                     /translation="MTTSENSVGPQVISSVPEASRPAKSHVRWTVCALLFCATSINYM
                     DRQVLGILAPVLQHSIGWTEAQYGYIVSAFQFAYAAGLVLAGRMVDRFGTRIGYAVVM
                     GIWSISAMCHSLVRSAFGFGVARFFLGLGESGNFPAAIKSTAEWFPQSERSLATGIFN
                     SGANVGAILAPAIVPWVTIRFGWRVSFLITGVFSATWILWWLTHYRKPAEHPKVSRRE
                     LDLIHRDPPQPTIHIPWKKLIAYRQTWAFSLGKFLTDPIWWFYLYWLPKFFDARFHLG
                     LSHLGLPLIIVYNASAVGSIGGGWLPALIHRTGMDMVKARLTAMLLCAVAVTPIFFAS
                     STKSEWVAVGLLSLAAAAHQGWSANLFTTASDMFPKTAVGAVVGIGGMAGSVGGVLFS
                     LSAGWVLQITHSYASLFGIAASAYLIALVVVRLLAPGLKKVEVSV"
     gene            8409..9713
                     /locus_tag="VFA02_00410"
     CDS             8409..9713
                     /locus_tag="VFA02_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZU08336.1"
                     /translation="MVQSTLAKEAMPLYLNAGNSTAEFTPLQLKEMLFEALSALGERK
                     RVLVVPPDQTRAHSRAGELTRYTWEYYGDNLKAVLPALGTHSAMSATAITRMFGDVPQ
                     SLFYVHNWRTDIVTLGEVPAEFIHEKSEGKLNYTWPAQVNKLIARGDFDLVLSIGQVV
                     PHEVIGMANYNKNILVGTGGREGINRSHYLGAVYGMERIMGRADNPVRQVLNYASDHF
                     LKNIPIVYVHTVVSRNAEGRLAVRGLFIGDDVECFHRAADLSLEVNFEMLDKPIQKAV
                     VYLEPHEFKSTWLGNKAVYRTRMALADGGELIILAPGVEEFGEDPAIDKLIRKYGYLG
                     TPNTLAAVEKNSDLASDLSAAAHLIHGSSEGRFNITYCPGHLSKEEMESVGFRYADLA
                     SMTKRYDPDKLRNGYNTVDGEEIFYISNPGLGLWAYRGRFHS"
     gene            9732..11006
                     /locus_tag="VFA02_00415"
     CDS             9732..11006
                     /locus_tag="VFA02_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HZU08337.1"
                     /translation="MSTHLTLPKYSIGVGDRFAHQARAQLAACVLAANAGIEIVPVWN
                     KSNREHTIIGSEPSSTRAAADAAVKALGWNKPYFCDADHIGLATVDRFLPACDFYTID
                     VAEEIGKPAGKAAVEAFVKRHPELQGEIKVEGISEPLTITREFLERVANKFLAAVQQA
                     GAIYRKIEAAKGAGNFITEVSMDETDSPQTPAELLIILVAAADEKIPLQTIAPKFSGR
                     FNKGVDYVGEVAQFEKEFALDVAAIAYAVKHYGLPQNLKLSVHSGSDKFSIYAPIHRT
                     LQRFNAGVHLKTAGTTWLEELIGLAEAGGAGLALAKEIYAQAFDHREELCAPYATVID
                     IDPAKLPSPQHVAQWTSAQYTSALRHDPKSTAYNSSFRQLLHVGFKIAAKMGNRYLDL
                     LEANEGVVARNVTENLYVRHIAPVFSGKGQLA"
     gene            11003..11776
                     /locus_tag="VFA02_00420"
     CDS             11003..11776
                     /locus_tag="VFA02_00420"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU08338.1"
                     /translation="MNHPMGLEGKTAVVIGGTSGIGRALSLGLAEAGADVVASARRKE
                     QVEETAAEIEACGKRTLRVSSDVSDRPSLENLLSQTLEHFGKADILINCAGKIKRAPT
                     LDFPEEEWQSIIDTNLTGTLRACQIFGKHMLERGYGRIINIASLNTFVALSEVAAYAA
                     SKAAVASLTRSLAVEWSKHGVLVNAIAPGVFRTALNAQLLDSTPRGKELLMRTPMGRF
                     GKTEEVVGAAIYLASDAASYVTGQVLVVDGGFLASGVNQ"
     gene            complement(11803..12216)
                     /gene="atpC"
                     /locus_tag="VFA02_00425"
     CDS             complement(11803..12216)
                     /gene="atpC"
                     /locus_tag="VFA02_00425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZU08339.1"
                     /translation="MANQLHIRLVTPDRVLVDSTADAVELPARNGYMEALFGHAPLLS
                     ELGAGEVRTHGGRAGEQRFFVSWGFVEVLPDRVTILAETALKPEEINASEAQQELDRG
                     QKLWSEAGDDAEKYEEANQVIEGAEAKLASASSRS"
BASE COUNT         2729 a         3512 c         3393 g         2670 t
ORIGIN      
        1 ccggccgcgg ccagaaataa ggcggacatg gccacccagg gactttgcag tacaacactc
       61 agaccgaatc cggctgctgc gcccgtgagt gaagcgaaaa gatgccatcg gcgctcctgc
      121 ttctcatctg aagagcgtgc aatcagtagc atagcaatcg cggccagcag atagggaatg
      181 gctgtcagga ggatgacgcg cagatctgaa gcccgcgaaa gctgcttcaa aaccacaggc
      241 agccacagac tcaggctgta aagagcaaca gagattgcga agtagataaa cgccagttgc
      301 cacaatactg cattacgcca aacatcgcgg agatggacag aatgcgaagc tgagatactc
      361 acctgctccg cagccagtgt ttccgtcagc caggtgcgtt catcaggccc aagccaggca
      421 gcccgttcgg gagaatcagt cagcaccaga aacacgagtg ctccgagcag aacagagggc
      481 agaccttctc caacgaagag ccatttccat ccggccacgc ctgcaacccc atcgagccgc
      541 agcagaaggc ccgacaacgg agcaccaatg acgccggcaa tggctgtagc cgccatgaac
      601 aacgccacag cacgggcccg ctgcttctgc ggaaaccagt atgtgagata aaggatgatt
      661 cccgggaaga agcccgcctc tgccgcaccc agtagaaacc gcagcagata aaactcagat
      721 ggggtgcgca ccagcggcat actggcagaa atcacgcccc atacaaccat cagagtgccg
      781 atccagcgcc gggggccgag tctcagcaga acaagattgc tgggcacctc aaagagcgca
      841 tagctcagga agaagagccc cgcgccggtg ccatacaccg tatcgctgaa gcccagttcg
      901 cggcggatga aaagcgctgc gtagcccaca ttgacgcggt cgagatacgc aaagacatac
      961 agcgcaaaaa gaaaaggcag caatcggcag gaaacgcgtc gaagcgttct tgccgaaagc
     1021 tgctctgttg tttccatgca gaatcttact ggctgatttt cagcatcacc acgccatggc
     1081 cgggcacgag cacggaatag ctgccgttcc tggctgttac gtcttcatgc ttccaaagat
     1141 cgcggagctt tgcattcgag ctaaagccaa catctttcaa ttgcagcgtg atgttacgtg
     1201 gagaatccgc gcggttgaac aggccaacgg cttttgctcc accggagagc ggcttggccc
     1261 atatctcctt cggaccttct tcccacacgc ggtctccctg cttgcccagc ttgtcctggt
     1321 ctacggcaat tacttctttg ttcgtgagaa tatccaatgt ttcttttgac attttgctca
     1381 gatcatttcc ggcaagaagc ggcgcggcga gaatcgccca tagcgacata tgcgtgcgat
     1441 actcctctgt tttcatcccg ccattgccta cctcaagcat gtcagggtcg ttccagtgcc
     1501 ctggtccggc atattttgag agtcccgcct gcgagaaccc aatctcagcc atgcggctat
     1561 agcggtcgga aatgtcgcct gtcgtgcgcc acagattgcc gcctacctcg ggcccccact
     1621 tccatacatc gttccatcca tactggcaga ggctgaagac gatcggacgg cctgttgcct
     1681 caagcgcctt gtgcatcttt tcataagcgg ccttctgcat ctcaaaggcc ttttcgctgt
     1741 gcgggtcgcc ggtctctttc ttcatgattt cgataaagct gcacaggtcg tacttcagat
     1801 aatcaatacc ccacgcggcg taggttttcg cgtcctgttc ctcatggccg tagctgcctt
     1861 cgtatccggc acaggtcttt ggccccggag aagagtaaat cccaagcttg agtcctttcg
     1921 agtgcacata atcggccagc gccttcatgt caggaaactt gctgttggtg tggatgacac
     1981 cattggcatc acgctggccc tcccatgtgt cgtcaatgtt cacatagatg tagccggcat
     2041 cgcgcatacc gctggagacc agcgcatcag cggccgcgcg gacgtcagcg tccgtgaccc
     2101 tttcggcaaa gtggttccaa ctgttccagc ccatcggcgg agtagcggca atttgagatg
     2161 actgcgccag catccatata ggagcagcaa gcagacccat tccacacaca agagaggcga
     2221 ttttgttttt aagcatgctt tccgtggatt ctccttccac aaagcaagat attcgctgga
     2281 gatgaatatg taaacgtaaa catgattcat tcaaaaaatg ggataatgtt gcggtctgct
     2341 ctgcgaggcc atacatgaag ctccttctga agatgctcgc cctctgcgtc gctactgcca
     2401 ccgtatatgc acaagctact cccagagatt cgctgctggt gctgtccaaa gtggaccgaa
     2461 cgctctcgat tgtggatccg gtcagcctga aggtcatggc acgtatcccg gcaggaaatg
     2521 atccgcatga agtagcggtt tctgcagacg gcaagcttgc ctttatttcc aactatggat
     2581 acggcgcgta caacaccatc acagtagtgg accttgcagc ccggcaaggc ctgaacacga
     2641 tagacctggg gccattacac gggccacacg ggctcgcatt tgcagaaggc aagttatggt
     2701 ttacggctga agtggccaaa gccatcgcca gatacgatcc ttcaaccaac aaggtggatt
     2761 ggatactcgg cctgggtcag aaccgtactc atatgatcta tgtttcgcag gacgcatcgc
     2821 gaatcattac cacgaatgtg aattcaggaa cagtcagcgt gattgatcga gtagcggtcc
     2881 atccccaggg gccagttgcc ggagcgcaga cccataggcc caattacacg cagccacatg
     2941 gccccgcgtt tgactggaat gaaaccttta ttccagttgg taaggggccg gaagggttcg
     3001 ccgtttcgcc tgacgggaaa gaggtgtggg ttgggaatgg actggacgga acagtatcca
     3061 tcattgacat tgacgcaaag aaggtcatag agaccatttc tgcgaacata ccaggcatca
     3121 accgggtgaa atttactccc gatgggaggc tggtttttat ctccagcctg cgcagcccga
     3181 atgttaccgt cttcgatgcg gccacacgca aggaaatcaa gcgaattcct gtcgggcacg
     3241 cctcggaagg catccttgtg cagccggacg gcgcccgcgt atttatctcc tgctcgcccg
     3301 atgactatgt agctgtaatt gatacaaaat cactgcggac cacgggccgt attgaagccg
     3361 gacatgaacc cgatgggctt gcctgggccg tccagcatta acacctgccg cgctgcgcgt
     3421 tgtccggaac gcaatgcgcc atgtacggtg ccccaatgcc cggtagtgtc cgtgtgttcg
     3481 ccagcgaaga agagagtatc ttctacggga atgctcatga cttctgaagc ttcaagcgcg
     3541 cccttgggag catagctata tgcccccaga ctgaatggat cgcgctgcca gtcatgcgtg
     3601 tgccagctta ccagtaattc gcgcaggtgc tcttccggaa cagaaaatat cttagccagc
     3661 gttcgcaaac cgagagcagc aaaatctgta tcaggaaaaa gggaagcatc cagagatttc
     3721 ggtccgccaa tccatccggt gagcatcggg gttagatcag gagacggcat ccaccagacg
     3781 cgcatcaggt cctgctgaga aaacagaaaa tgcgcattcg ggaacttcat ttgccagaag
     3841 cgttcacgaa acaaaaacgt aatgcgtttg gcgctgccca tggccatttg cgagacagcg
     3901 tggaatgtct tagatggtga tggggcaaaa tgaatgctgc cggactgcaa tactcccagc
     3961 gggactgtca caatgcactg gtctgcttca aaaacacgtt cctgatactg ctgcatcgcg
     4021 tggatgcgaa catttccgcg ccgccatgcg acagaaatca caggcgcaga aagaaagact
     4081 tcgccgccgt ggctctgaaa ttgtttcaac agaaatgcgg gcagagcact gtaaccttcc
     4141 ctcaacctga aagcgagatg gccttgaatc gcgtcttctg ccgcctgctg ctttgccagc
     4201 gccgctgtcc cgattctggc agcatcggcg gcattaaagc cttcgacaaa ggcaatggcc
     4261 gattcaacaa taggagcagg cagctcttgc tgcgcaagcc attcggcgaa gctctggtct
     4321 gggccattgt caatcagccg acgcatcaga tcaaagccgc ctggcccctc gtcgcattcg
     4381 tgcaggctgt tttcatcaaa gcagagcatt gtgccatcca tttcataggc atggagaccg
     4441 gcctcttcga taagctgcca cacttctact gggcggccat gaataaattc cgctcccagc
     4501 tctacaggaa gatcagatct ttgttgtgga agtgagaaaa tccgtccgcc aatgcgcgaa
     4561 cgtgcttcaa tcagagcaac tcttttgcct tgggtagcca atatccgcgc acccgtcaga
     4621 ccagcgatgc ccgcgcccag aacggccaca tcatatttct caagtcccat gcagattgga
     4681 tgtcaggcgc tggcgcgagc gctcttcttt ttcgccgcgg gacgtttttc ctctttggcc
     4741 agggcctgct cgtttacctg tgctgactcg gccatgtgca gatgccgttc catcacttca
     4801 cgggcctccc ttgcattgcg agagcgaatg gcgcggtaca actcgcgatg cacttccgct
     4861 gattgcttca ggtcacgtga gcgctctacg gttttgcggc gttcgtcata gagggctgcc
     4921 gtgatggtct ccatcagcac ggcgagaatc ggattgccgg atgcctcggc aatggtgcga
     4981 tgaaaccgca tgtcgtggat gaggtattcc tgcggttcat ggaaggtcgc gtacatttcc
     5041 gtgacctctt ccgccagcgc agtaaagtgc tcttcgcgcc cgcgctctgc ggcaagggct
     5101 gcaaggtggc tttcgagtat ccgccgggcc tcgaacatct gcgctgtcgt gtatccatgt
     5161 aacgcacgca gaatgtcgag cgaaacaccg cccagagctg gaggtccatc ggcgacgaag
     5221 gtccccacgc catggcgcac gttcagcacg cccatggccg ccaggtggcc gattcctgcg
     5281 cgaaggctgg cacggctgat tttgagcttc tgcgcaaact cgcgctctgg cggaatcttg
     5341 tcaccgggcc gcagcgttcc attttcaata agccggcgga tgtggttgac cacctgcatg
     5401 gtcaaatggg tctcgtggtt atgattgggt tcgctcattg ccatattgcg tcttgccaga
     5461 gagattagca ttgtcacgtg gacatagcgc actactttct tagcagattt gaaaatttag
     5521 agatagacta acctggagtg gttagaccac tgccaccacg aggcccactg tatggcgatt
     5581 ctccacgaag accgactttt tcccgccgac cctgagacgc gaaagattgc gcgccgcctg
     5641 tatgcaaccg tccgcgacct gcccatcatc agcccgcatg gacatactga agccgtctgg
     5701 tttgcaaaga atgaaccatt ccctgacccg gcgcagctct ttatcctgcc tgaccattat
     5761 gtataccgaa tgctctacag ccagggcgtt tccatgcacg agctaggtat tcgccagccc
     5821 aagccgccgg acccgcgcaa aatatggcgt atctttgccg aaaattatta tctctttcgc
     5881 ggtacgccga cccgcctgtg gctcgactat gagtttgaga cattgttcgg attaaaagac
     5941 cgcctgtctg cgaaaaatgc tgaccactac tacgatgtta ttgctgataa gctccagacg
     6001 ccgcagttcc tgccgcgcgc actatacgag cgcttccgga ttgaagtgct tgcaacaacg
     6061 gattctccgc tcgatacgct tgtacaccat cgtgcccttc gcgaatccgg atggaaagga
     6121 cgaattgtgc ccaccttccg gcctgatcca gttgtagacc cggcctttgc aggcttccgc
     6181 gagaacatcg cgaaattggg cgaactgacc ggagaagata catcaaagtg gagcggctat
     6241 ctgaacgcgt tacgcaaatc gcgcgaacgc ttcaagcaac ttggctgcac tgcaaccgac
     6301 catgggcatc ccacagccaa taccgccaac atgagcacgg cggaagcatc ggctttgttt
     6361 gatgttgtgc tctcgggcaa gtcttcgccg ccgcagcagg agaccttccg tgcgcagatg
     6421 cttaccgaaa tggcccggat gagcgtggaa gatggcctcg tgatgcagat ccatccaggc
     6481 agcttccgaa accacaatca gaaagtctac gagcagtttg ggcgcgatgc gggtgcagac
     6541 ataccgacgg ccaccgacta tgtgcacgcg ctgaagccac tgcttgacct cttcggaaac
     6601 gaaccctcgt tgaccatcat tctctttacc ctggacgagt ctgcttacag ccgcgagttg
     6661 gccccgttgg ccggacatta tccatgtctg aagctggggc cgccgtggtg gttctttgac
     6721 agtccagagg gaatgctgcg cttccgtgaa ctggctacgg agacggccgg tttctacaat
     6781 accgttggat tcaacgatga tacgcgcgca tttctctcta ttccgtcgcg acacgatgtg
     6841 gcccgcagaa tggattgtgc ctttcttgct cgcctggtcg ccgagcaccg cctcgatgag
     6901 gacgaggccg cggaggtggc gcaggatttg accgtcaacc tggtccgcaa ggcataccgc
     6961 ttatagagac atagtgggcg gcgtgtcttg cccatcgtta cagcacacag atataacggg
     7021 acagactcaa ttgcatgact acttctgaaa actccgtagg accgcaggtg atttccagcg
     7081 tgcctgaagc atcgcgcccg gccaaaagcc atgtgcgctg gacggtctgc gccctgcttt
     7141 tctgcgcgac ctccatcaac tacatggatc gccaggtgct gggcatcctc gcgccggtct
     7201 tgcagcacag catcggctgg actgaggcgc agtatggcta catcgtcagt gccttccagt
     7261 ttgcttacgc cgctgggctg gtgcttgcag gacgcatggt agacaggttc ggcacgcgca
     7321 tcggttacgc cgtcgttatg ggcatatgga gcatctcggc gatgtgccat tcgctggtac
     7381 gctcggcctt tggctttggc gttgcgcgct tctttctggg gctgggcgaa tcgggtaact
     7441 tcccggcggc catcaagtct acggctgaat ggtttccgca aagtgagcgc tcgctggcta
     7501 caggaatctt caattcaggc gcgaatgttg gcgcaattct tgctcccgcg attgtgccct
     7561 gggtgaccat tcgcttcgga tggcgagttt catttttgat tacaggcgtc ttcagcgcca
     7621 cgtggatcct ctggtggctc acgcattacc gcaagcctgc cgaacacccg aaggtcagcc
     7681 gcagggaact ggaccttatc caccgcgacc cgccgcagcc cacaattcac attccatgga
     7741 aaaagctgat tgcctatcgc cagacgtggg ccttctcact gggaaaattt ctgacggatc
     7801 cgatctggtg gttttacctt tactggctgc ccaagttttt tgatgcgcgc ttccatctgg
     7861 gactctcgca tctcggcctg ccgctcatca ttgtctataa cgcatctgcc gtcggcagca
     7921 tcggcggagg gtggctgcct gctttgattc atcgcacagg aatggacatg gtaaaggcgc
     7981 gccttacggc catgctgctc tgcgcggttg ccgtgacgcc catttttttc gccagctcca
     8041 cgaaatcaga atgggtggct gtaggcctgt tgagcctggc cgcggccgcg caccagggat
     8101 ggtcggcgaa cctgtttacc accgcctctg atatgttccc aaagaccgca gttggggccg
     8161 tggtcggcat cggcggcatg gctggatcgg tcggcggcgt actgttctcg ctcagcgccg
     8221 gatgggtact gcaaattacg catagttatg cgtcgctgtt cgggatagcg gccagcgcgt
     8281 atctgattgc ccttgtggtc gtgcggctcc tggcccccgg actgaaaaaa gtggaagttt
     8341 ccgtctgatt tcagacaagt ccgccgaatt ctcaaaagtt tctttcgtaa tccagtggtc
     8401 ataccactat ggtccagagt acactggcaa aggaagccat gccgctttat ttaaatgccg
     8461 gaaacagtac ggcagagttt acacctcttc agctaaagga aatgcttttc gaggcgctaa
     8521 gcgcgttggg agagcgcaag cgcgtactcg ttgttcctcc agaccagacg cgcgcccatt
     8581 cccgcgccgg cgaacttacg cgttatacgt gggagtatta cggcgacaac ctgaaggcgg
     8641 tgctccccgc gctgggtacg cattcggcca tgtccgctac agccattacg cgcatgtttg
     8701 gcgatgtgcc gcagtcgctt ttctacgtgc acaactggcg gacggacatt gtgacgctgg
     8761 gagaggtccc ggccgagttc attcatgaaa aatccgaagg caagctgaac tacacgtggc
     8821 ctgcgcaggt gaacaagctg attgcgcgcg gcgattttga tctcgtgctt tccataggcc
     8881 aggtcgtgcc gcatgaagtc atcggcatgg ccaactacaa caagaacatt cttgtgggca
     8941 cgggcggacg cgagggcatc aatcgcagcc actatctggg cgcggtctat ggcatggaac
     9001 gcatcatggg acgcgcggac aacccggtgc gccaggtgct gaattatgcc tcagaccatt
     9061 tcctcaaaaa catccccata gtttacgttc acaccgtggt cagccgtaac gccgagggcc
     9121 gtctcgcggt gcgcggactg ttcattggcg atgacgtcga gtgtttccat cgcgctgccg
     9181 atctgagcct cgaagtgaat tttgagatgc tggacaaacc cattcagaaa gcagtcgtct
     9241 accttgagcc gcatgagttc aaaagcacat ggctggggaa caaggcagtt tatcgcacac
     9301 gcatggcgct ggccgatggc ggcgaactca tcattcttgc acccggagtt gaggagtttg
     9361 gcgaagatcc ggcgattgat aagctcattc gcaaatatgg atatctcggc acgccaaaca
     9421 ccctcgcagc ggttgagaag aattccgacc tggcatcgga cctgagcgcc gcagcgcacc
     9481 tgattcatgg ctcgtcggaa ggccgcttca acatcacgta ttgtccggga catttgagca
     9541 aagaagagat ggagagtgtc gggttccgtt acgccgatct agccagcatg acgaagcgtt
     9601 atgaccctga caagctcagg aacggataca acaccgtaga tggcgaagaa attttctata
     9661 tctccaatcc tggattgggc ctgtgggcgt atcgcgggcg atttcattca taaatcaagg
     9721 agcatgatga aatgagcact catcttacgc taccgaagta ttccatcggc gtaggcgaca
     9781 ggtttgcgca tcaggccagg gcacagcttg cggcgtgtgt tctggccgca aatgcgggca
     9841 ttgaaatcgt tcccgtgtgg aacaagtcga accgcgagca cacgatcatc gggtctgagc
     9901 cttcgtccac gcgggccgca gccgatgcag cggtgaaggc gctcggctgg aacaaaccct
     9961 acttctgtga tgccgaccac atcggactag ctacggtaga ccgctttctg cccgcatgcg
    10021 acttctacac cattgacgtc gccgaagaga ttggcaagcc cgccggcaaa gccgccgtgg
    10081 aagcatttgt aaagcggcac cccgaactgc agggcgaaat caaagtggaa ggcatcagcg
    10141 agcctctgac catcacgcgc gagtttctgg agcgggttgc caataagttt cttgccgcgg
    10201 tgcagcaggc cggggccatc tatcgcaaaa ttgaagcagc aaaaggggca gggaacttca
    10261 ttacggaagt ctcgatggat gaaaccgatt ctccgcaaac gcccgccgaa ctgcttatca
    10321 tccttgtcgc tgccgcggac gaaaaaatcc cgctgcaaac catcgctcca aaattcagcg
    10381 gacgcttcaa caaaggtgtg gactacgtgg gcgaagtggc gcagtttgag aaagaatttg
    10441 cgctcgacgt ggcagccatc gcctatgcgg taaagcacta tggacttccg caaaacctga
    10501 aactcagcgt gcattcggga agcgacaaat tttccatcta tgcgcccatt caccgcactc
    10561 tgcaaaggtt caacgccggg gtgcatctta aaacagctgg cacaacgtgg ctcgaggaac
    10621 tcattggcct ggcagaagca ggcggcgccg gactcgcgct ggccaaagag atttatgccc
    10681 aggcgttcga tcatcgcgaa gaactctgcg caccatacgc aacggtgatt gatattgatc
    10741 cggcgaagtt gccctcgcca cagcatgtcg cgcagtggac ttctgcgcag tacacctcgg
    10801 ccctacgcca tgatccgaag tctacggcct ataacagcag tttccggcaa ttgctgcatg
    10861 ttgggtttaa gattgccgca aagatgggca atcggtatct tgatttgctg gaagcgaatg
    10921 agggagtcgt ggcgcgcaat gttacggaga atctatacgt gcggcacatc gctccggtct
    10981 tttcagggaa agggcagctt gcatgaacca tcctatgggt cttgaaggaa aaacagccgt
    11041 cgttattggc ggaacatcgg gcattggacg cgccctcagc ctggggctcg ccgaagcggg
    11101 agctgatgtt gtcgcctctg cgcgccgcaa ggagcaggtg gaagagaccg cagcggaaat
    11161 cgaagcgtgc ggcaagcgta ctctccgcgt ttcgtctgat gtaagcgacc gtccttctct
    11221 tgaaaatctc ctcagccaga cactggagca cttcggcaag gccgacatcc tcatcaactg
    11281 cgcaggaaag atcaaacgcg ctcccacgct cgattttcct gaagaggaat ggcagagcat
    11341 catagataca aacctgaccg gaacgctgcg cgcctgccag atattcggca agcacatgct
    11401 tgagcgcggc tatggacgca tcatcaatat cgcctctctc aatacgtttg tggcgctctc
    11461 tgaggtcgct gcctacgccg cgagcaaagc agccgtggct tcgctgacgc gctcgcttgc
    11521 cgtcgaatgg tcaaagcacg gcgtactggt gaatgcgatt gctcccgggg tctttcgcac
    11581 agccctcaat gcgcaactgc tcgatagcac tccgcgcgga aaagaattgc tgatgcgcac
    11641 gcccatgggt cgcttcggta agacggagga agtcgtcggc gcggcaattt atcttgcttc
    11701 cgatgcagct tcgtacgtta ctgggcaggt gcttgtagta gatggagggt ttctggccag
    11761 cggagtaaac cagtaggaaa aggaaagctg gatccagcaa ttctacgagc gtgaagaagc
    11821 gctcgccagc ttcgcttcgg cgccttctat cacttggttg gcttcttcgt atttttctgc
    11881 gtcgtcgccc gcttcgctcc acagcttctg tccgcggtcg agttcctgct gggcctcgct
    11941 tgcgttgatt tcttccggct tgagcgcggt ttccgcaagg atagtcacgc ggtccggaag
    12001 cacctccaca aagccccagc ttacaaaaaa gcgctgctcg cctgcgcgcc ctccgtgcgt
    12061 gcggacctca cctgcgccaa gctcagaaag cagaggagcg tggccaaata gcgcctccat
    12121 gtaaccattg cgcgcaggca gctcgaccgc gtcggcggtg gagtccacca gaacgcggtc
    12181 cggggtcaca agccggatgt ggagttggtt cgccatggtc ttatgcgttt gccttcatct
    12241 tttctgcggc ttcgagtacg tcttcaatcg agcctttcag atagaacgcc tgctccggaa
    12301 tatc
//
LOCUS       DASRBV010000015        16548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_836,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000015 DASRBV010000000
VERSION     DASRBV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..5059
                     /locus_tag="VFA02_00430"
     CDS             20..5059
                     /locus_tag="VFA02_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HZU08340.1"
                     /translation="MFYYNGKYYLSTEGTDPTQQWVTIVYSSSSPKSGFTLVPGNPVL
                     ANGGACMFQQAFGNTLYNYNCQQTNGAWTLQMRTASLSAGPQAFGTFNPSKWKISGGG
                     VWAIVPATQPDGTQGHVAQAQTSSRQVLVTPNYSAGDYVVQASGKEILGDVWGVGVHA
                     TAWSNLYSVNLYGDMNGGYNLYSYGWFNNTSDIANTQILQLQAGNIQPNTWYKIVTKV
                     HQGTIEVDTNGQSAQGSDSTLATGGVALYAEGGTNAEWDNLLVRKYAIVDPTASVGQA
                     TQAGISSLTVSPSSVLGGATSQGTVTLAIAAPAGGATVSLSSSVSSASVPASVTVPAG
                     GTTATFTVTTTSVATVTSAKISATYGSSGQTTTLTINPYLSTVSISPSTVQGGSSSQG
                     TVTLYAAAPSGGATIGLSSGNSAATVPGSIVIPAGQTSGTFTIGTSAVNTTVAAQITA
                     NYAGASVSSTLTIQPPLSSFAIAPGTIIAGATAQGTVTLGSAAPSGGAVISLSSNNAA
                     AIVPASITIPAGSTSANFTVNGGSTAANATATITASYMGSTQTASITVLPTLASLTLS
                     QNNIFGGNSTQGTVTLNGAAPSGGTVVNLSSNNSAASVPASVTVAAGTTSASFTVTTT
                     TVSSNTTANITASSFGSSQAASLTIAPASGNWFSPSWQYRSAVTITNSNSSALSNFQV
                     KVQLGSNFDFTKAAANGADIRFTASDGLTAIPFWIESWNSTSAVLWVNVPSIPTSGTT
                     IFMYYGNPSATSASNGPATFLFFDDFSGGNISSSKWTSTGGTWSVISDTNENGVTSSV
                     AQGATTNRQILYSTYSGTDYVADVFGKQTAGRVWGLGVRATNASNLYSINLYDDLNGG
                     TNLYAYSWVNDPGGNATATLGGGNAGTVTANNWYRLTAKVHGNAIDILANNVPVVSVT
                     DSSNALPSGGIALYGETGTTAEFENVLVRQYAATDPATSVGPTTTQGGGTTGGGGGNA
                     RVYRICLTQAEVNGGASSPATCTLPSGVNVPAGHGVVVFVSFPSTTTIATNAVSDTEG
                     NTYTYLTTQQDEGTADTIWAWSSVLKNPLNAGDIITLVPPQFWGAWNLYIYDIGPVTG
                     TDTYAADPLYYNTSWTLGPTSATTGPKDVCIGAAGVNQVPTPPISDFSVTNNFTLLDV
                     TNFHNPQSANYPGKNLVTMWGEVTSGTQVSTTLTSTIGPDTGASILGCWIEASTSQTQ
                     AQIQVQPSQITFSDAVVGTTTTAQGVTVSNTGNASASLQSSSVSGDFQISGSTCGTAL
                     APNTSCTVSVAFTPKASGSRSGSLSIQDNLGTHLAQLTGRGLTQATADLSASGLNFFL
                     PVVVGQKSAAELITLANNGDAVLGQIAVSISGDFTMDNDCGTQLAGHTSCPILVTFAP
                     TQAGVEQGVLQVNTVFGAQQVRLSGTGMSPPALFLASTLPGFGSQGIGTASSTQFITV
                     VNRGGSTLTGLTFSISGDFTISTNTCTPGAALTSGSACILGVVFTPTQTGTRNGTLAV
                     SGANLASPLIEPLQGTGIDFELTILGQTSQTIQSGQSAKYQVQFTFIGGPGGVPYITC
                     SGLPKNASCSIDQSAGAVSTANAQTINATVTIAPAATTASTPEKTGLTLAFFLPFFAG
                     FARRRNLMRAVLLCFVSAMLLASAACGIQPNSFSQTADASGLQAGVYTIHISASLDSL
                     TKTVPVTLTVK"
     gene            complement(5120..6163)
                     /locus_tag="VFA02_00435"
     CDS             complement(5120..6163)
                     /locus_tag="VFA02_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU08341.1"
                     /translation="MVFSTGTKSPHTIEINLPPPDVIFGTTAAMKIVQLKLERAAGRN
                     IPVLIRGESGTGKEVIAKLLHHLSPWKHGPFVKVNCPAIPGTLMESELFGYERGAFTG
                     AYASKPGKVESARAGTLFLDEIGDLEPNLQAKLLQVLQDGRFCRIGGETEQHVDVRIV
                     SATNQNLEENIAAGSFREDLLYRLDGVTISLPPLRERRNDIPRIVEFLIEHHSEHLNC
                     MARPLSSSAVQALMDYPWYGNIRELENIVRRYVIFGQEDAVLADLTRGNAPFFAVTTS
                     FDGDFSLKRITRQTVQDLERRLILQVLASNNWNRKKAARVLKISYRSLMYKIREMNIP
                     PLRVKDAQQADSI"
     gene            6589..8040
                     /locus_tag="VFA02_00440"
     CDS             6589..8040
                     /locus_tag="VFA02_00440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU08342.1"
                     /translation="MTTAQSLSAVSQETDLSAKSGLEGAQIFLIDQAGVMEHLRSLLS
                     GHSCRVTGVSRSEDAMAKLKTSQRPNVILIDAHLPGPGGLSVLSAVREMYPSVPCVML
                     SCSYDPRLIVEAMKRGAVDIILKPFHKNDVLETLRRCIAIVDSSYENQAREIPLVDNT
                     SFVFASERMRTIFSQCSTIARVDLPVLILGESGTGKEVLAQFIHKMSPASHRTFLKVN
                     CAAMPADLLESELFGYEQGAFTGAVKAKPGKFEMCNNGTIFLDEIGEMPFPLQAKLLH
                     VLQDGSFSRLGGRTTMKVNVRMIAATNIDMKVAIAERRFREDLFYRLNGFSLHLPPLR
                     ERKDEIPVLVRHFVAKLSDKYAQKCPTVSDEMLRACMNYAWPGNIRELENFVKRFLVL
                     GDERMMMAELRGDRKLPAASGIGGQPEPAAMSLKKLVRRVKADAEAEAISAALDRNNW
                     SRRQAAAELKISYKALLYKMRDYGLSEPQAMRA"
     gene            8055..8567
                     /locus_tag="VFA02_00445"
     CDS             8055..8567
                     /locus_tag="VFA02_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08343.1"
                     /translation="MEEAAFVDVKEAKSTRKEEEKVVVHLTGGRIIKGFLPVESPKDL
                     QSLLEKPRLNFSGTITVFSAEGRTPVEIDAQQLKAIFFVNSFSGNSERNALRYYIHGP
                     EVGAIWVEVQFKDDEIMEGIVHNSIHHLIADGFFLHPSDPGSNNKLVYIHKNAIKDYR
                     VLGVRTLANG"
     gene            complement(8912..9160)
                     /locus_tag="VFA02_00450"
     CDS             complement(8912..9160)
                     /locus_tag="VFA02_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08344.1"
                     /translation="MLFKLWLPGALLLTAPFSSDWQFGHLLAIKQAQRGWKYLCKQKY
                     GTCPRSKNQNGKSITNQEENFVPEAIFGSAGLSAKVPV"
     gene            9374..11347
                     /gene="asnB"
                     /locus_tag="VFA02_00455"
     CDS             9374..11347
                     /gene="asnB"
                     /locus_tag="VFA02_00455"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZU08345.1"
                     /translation="MCGIAGIISQDSTALRTLALERMTEALSHRGPDEHGLHIEGSAA
                     LGHARLSIIDLAGGHQPMQLHDGRMWTTFNGEIFNYIELRQELEQKGHKFVTKSDTEV
                     ILHLYQEYGTDFVQRMNGQWALAIWDDARKQLFLSRDRMGIRPLYYTTVGGALIFASE
                     IKAILAHPRVPRQLDLQALDQIFTFWCPLPSRTTFKNIHQLPPGHSGIFKDGHLKTWR
                     YWKLNYEWQTDESPRAVERAADQLFELLLDAARIRLRADVPVGAYLSGGLDSAVTTAL
                     ITRLVPENLRTFSVGFEDADLDESVWQREAASFFGVGHESIRCSAEDIGEVFPDVIWH
                     MEQPVLRTAPAPLYILSRLVRESGFKVVLTGEGSDEILGGYDIFKEAKVRRFIARNID
                     SKFRPLLLRRLYPYIGNIQKQSPAYLQRFFHVNPSDVENPFFSHIPRWTLTARAKTFF
                     SDDVRQELGGYDCLSDMRSELPTEFSRWDGFSQAQYLETAHLLPNYILSAQGDRVAMA
                     HSVEGRYPFLDYRVVEFASHLSPALKMKALDEKYLLKRMAKGLVPESILSRPKQPYRA
                     PDGRSFVHGKAREYVDEILSAETIRNAGIFKPEPVRMLLEKFRSGRPTNTGDDMALVG
                     ILSTSLLMDQFIYQNRKQDCACTTYSESFATLS"
     gene            11308..11586
                     /locus_tag="VFA02_00460"
     CDS             11308..11586
                     /locus_tag="VFA02_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU08346.1"
                     /translation="MYNLQRELRDFIVTNFMFGDGAGLADNDSFINKGVIDSTGVLEL
                     VMHLEETYRIQVKDNELVPENLDSIDNLVAFISRKLAAEQELVTAAQA"
     gene            11599..12606
                     /gene="nadE"
                     /locus_tag="VFA02_00465"
     CDS             11599..12606
                     /gene="nadE"
                     /locus_tag="VFA02_00465"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZU08347.1"
                     /translation="MDQTENLAVLSHPLSLDVEAEAQRIEASIREMVFKRLKRKGAVI
                     GVSGGIDSSVVAFLCARALGPERVILLFTPELDSSSENLRLGQSVADALGVRSFLEDI
                     SPVLKGARCYERRDEAIRQVFPEYGPGYKMKIVLPSIDEDSKYSLFSLVVQAPDGAVH
                     KRRLTADAYLGILASTNFKQRARKMMEYHYADRYQFAVAGTPNRLEYDQGFFVKNGDG
                     SADFKPIAHLYKSQVYQLAEYLGVPEEIRRRPPTTDTYSLEQTQEEFYFSMPLQQFDL
                     CLFGKNNGLAPAEIADLAGLPAHAVERAYQQIESKRAVTHYLRMKPLMVEPVTECEIK
                     E"
     gene            12622..14175
                     /locus_tag="VFA02_00470"
     CDS             12622..14175
                     /locus_tag="VFA02_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU08348.1"
                     /translation="MSLATVSPEAIVRKEEYGSNVYDYLLAGKDPERVALILLDGEKT
                     YEDILFSSQLIASHLLQQGHSKGDRVLLIAENSFFWVASYLGILRAGLVCVPLPANTA
                     QETVSYIAEITEAKSACVPARVFLKNKLRLPVPVLTDVPVQEAEKPGAQHRNGFIQPQ
                     NFPQISRDDLAALMFTSGTTGKPRGVMVTHGNIIANTNSIIEYLRLSSRDRIMAVLPF
                     QYCFGASLLHTHLRAGGSLVVDPRFMFPEKILERMRETECTGFAGVPSHYQILLRKSA
                     LKDYCFPHLRYVQQAGGHLAPALIRELREALPGTEIFVMYGQTEATARLSYMPPEMLD
                     RKPGSIGKGVPGVKLRVIYAGRDAAPGEIGEIVAQGENITQGYWRAEEETATVFRDGM
                     LYTGDLATVDEDGYIFIVDRAKDFIKCGGNRVSCRQIEEKLLEFGDLLEAAVIGVPDE
                     ILGEAVKAFVVPRPGVVSDLEPLLRQFCTRNMLPAQVPREFVFLDALPKNASGKVAKS
                     VLREIRMAS"
     gene            14172..14969
                     /locus_tag="VFA02_00475"
     CDS             14172..14969
                     /locus_tag="VFA02_00475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZU08349.1"
                     /translation="MTRKLAVALTLVFLAISSCTKKEHPSQMISESQWSRIEGLRIYF
                     GHQSVGENILDGIRDIEKQSHRNSLRIVSSDDPSSIEGPALIESHVGRNGDPTSKNIA
                     FRDILDKGFGAQGGIALYKYCFVDIRPETDVAKMFAEYRDNMRDLRSRYPRLTIVHVT
                     IPLTTVEPGWKAVLKNLLGRPTERAIAEKRHQFNQLLRQEYAGKEPIFDLAEAESTLP
                     DGSRAYFISRGERIYTLAPEYTYDGGHLNESGRNVTALRLLQTLAQF"
     gene            complement(15034..15981)
                     /gene="fabD"
                     /locus_tag="VFA02_00480"
     CDS             complement(15034..15981)
                     /gene="fabD"
                     /locus_tag="VFA02_00480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZU08350.1"
                     /translation="MSDKKLAFLFPGQGSQAVGMGRDLYDKFPVAKQTFEEADEALGF
                     SLSKLCFEGPEEQLRLTEFTQPAIFTVSIAAQRVLAEKGITPGFAAGHSLGEYSANVA
                     AGVLSFADAVKTVRNRGRYMQEAVPAGQGAMAAILGLSSDAVIEVCAKAAAETEAVVS
                     AANLNSPEQTVISGTAAAVERAAALAKEAGARRAVMLPVSAPFHCALMQPAQDRLAED
                     LRRLNFSAPKVPVITNVDAALVTGAEALRDALIRQVTGSVRWVESMRLLIEQKPTHFI
                     EVGPGKVLCGLLRQIDRTQTALNVEDAASLEKTLAALHG"
     gene            complement(16132..>16548)
                     /gene="rnpB"
                     /locus_tag="VFA02_00485"
     ncRNA           complement(16132..>16548)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFA02_00485"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         3840 a         4393 c         4373 g         3942 t
ORIGIN      
        1 aaaaatggcc gcccccagca tgttttatta caacgggaag tactatcttt ctactgaagg
       61 aaccgatcct acccagcaat gggtcacgat agtgtattcg agctcttctc ctaaatccgg
      121 ctttactctt gtgcccggga atccggtcct cgcaaatggt ggcgcctgca tgttccagca
      181 ggcctttgga aacacactct ataactacaa ctgtcagcag accaatggtg cgtggacatt
      241 acagatgcgc acagcaagtc tgtctgcagg ccctcaggcc tttggcacat ttaatccttc
      301 caaatggaag atcagcggcg ggggtgtctg ggcaattgtt ccagccacgc agccggatgg
      361 aacacaaggg catgtcgcac aggcccagac ctcgtcgcgg caggtcctgg tcactccgaa
      421 ttactcggcg ggtgattacg ttgtccaggc cagtggcaag gaaatcctgg gtgacgtgtg
      481 gggggttggt gtccatgcaa cagcctggtc caacttatat tccgtcaatc tctatggaga
      541 tatgaacgga gggtacaacc tctactcgta cggttggttc aataacacca gtgatattgc
      601 gaacacgcag atactgcagc tgcaagccgg gaacatccag ccgaatacgt ggtacaagat
      661 tgtcacaaaa gtccaccaag gcacgatcga agtagataca aacggccaaa gtgcgcaggg
      721 ttctgattcc actctcgcta caggcggcgt tgccctgtat gccgaaggtg gcaccaatgc
      781 agaatgggac aacctgctgg taagaaaata tgccattgtt gatccaacgg cctctgttgg
      841 gcaagccacg caagcaggga tctcgtctct taccgtttct ccatcatcag tgctgggtgg
      901 cgcaacttcc cagggaacgg taacactggc gattgcagca ccagccggcg gagcgacagt
      961 ttccctttcc agtagtgtct cttccgcctc agttcctgcc agcgtaactg tgcctgccgg
     1021 cggaaccacc gcgactttta cggtaacaac aacatcggtt gcgacggtaa cctctgctaa
     1081 gatttccgcc acctacggca gttccggcca aaccacaaca ctcacaatca atccgtatct
     1141 ctctacagta tccatttcgc cctcaactgt gcagggagga agttcttcgc aggggaccgt
     1201 tacgctttat gcggcggcgc cttcaggtgg agcaacaatc ggtctgtcca gcgggaattc
     1261 cgcagcaaca gtgccgggca gcattgtgat tccggcaggc cagacttcag ggacctttac
     1321 gattggcacg agcgcggtga atacaactgt agcagcgcaa atcaccgcca attatgctgg
     1381 agcaagtgtc agctctacgc tgacaatcca gccaccgctg tcatcttttg caatagctcc
     1441 ggggacaatc atcgcaggcg cgacagcgca gggaacggta actctcggca gcgctgctcc
     1501 ttccggaggc gcggtcattt cactgagcag taataatgcc gcggcaattg ttcctgcatc
     1561 cattacaatt cccgccggtt caacttctgc gaactttaca gtcaatggtg gatctacagc
     1621 agcgaacgct acggcaacca ttactgcttc ttacatgggc agcacgcaga ccgcgtccat
     1681 tacagttttg cctacgctgg cttcgcttac actcagccag aacaatatct ttggcggaaa
     1741 cagtactcag ggaactgtga cacttaacgg tgcagcacct tccggcggta cggtggtgaa
     1801 tctatcgagc aacaacagtg cagcatctgt tccggcaagc gtgaccgttg ctgcagggac
     1861 gacaagcgcg agttttacag tcacaacgac cactgtttct tccaacacta cggcaaacat
     1921 tacggcttcg agcttcggat cttcgcaggc agccagcctg acgattgctc ccgcatccgg
     1981 caactggttc tcgccatcat ggcaatatcg cagtgcggtt acgattacaa attcgaacag
     2041 ctctgctctt tccaacttcc aggtgaaggt gcagcttgga tcgaactttg acttcaccaa
     2101 ggcagcggcg aatggggctg acatccgctt tacagcttct gatggactga ctgcaattcc
     2161 cttctggatc gagagctgga acagcaccag cgctgttctg tgggtaaatg tcccctccat
     2221 acctacatcc gggacaacca tattcatgta ctatggcaat ccgtcagcaa caagcgcatc
     2281 gaacggtcct gccacttttc tcttcttcga tgacttcagt gggggaaaca tcagttccag
     2341 taaatggacc agcaccggtg gaacatggtc tgttatctca gacacgaacg aaaatggtgt
     2401 gaccagcagc gttgcgcaag gggccacgac caaccggcag attttgtatt ccacctattc
     2461 aggaacagat tatgttgccg atgtattcgg caagcagact gccggacgtg tttggggcct
     2521 gggtgttcgg gcaacaaatg cctcaaacct gtattcaatc aatctctacg atgacctgaa
     2581 tggaggcacg aatctttatg cctacagttg ggtcaacgac cctggcggaa atgcgacggc
     2641 cactcttggc ggcggaaatg ccggaacagt caccgcaaac aattggtacc gcctaacagc
     2701 gaaagtccac ggaaacgcaa ttgatattct tgcaaataat gttccggtcg tttcagtaac
     2761 agacagctcg aatgcactgc cttcaggggg aatcgcgctc tatggcgaaa caggcacaac
     2821 cgcagaattt gagaatgttc tcgtccggca atatgccgct acggacccgg ctacaagcgt
     2881 tggccccacg acgacccagg gcggaggaac gaccggaggt ggcggcggta acgcgcgtgt
     2941 gtatcgcatt tgccttacgc aggcagaggt caatggagga gcaagttctc cggcaacctg
     3001 cactctgccc agcggcgtaa atgttcctgc cggacacggt gtggtggtct tcgtttcgtt
     3061 cccaagcaca acgacgattg caaccaatgc cgtctcggac acagagggca atacatacac
     3121 gtatctcacg acccagcagg acgaaggtac agcagatact atctgggcat ggtcttctgt
     3181 gctcaagaat cccttgaacg ccggcgacat catcactctg gttcctccgc aattctgggg
     3241 cgcatggaac ctgtatatct atgacattgg tccagtgacg ggcacagata cctatgccgc
     3301 tgatcctctg tactacaaca cgtcgtggac gcttggtcct acttccgcaa ccacgggacc
     3361 gaaggatgtc tgcattggtg cggccggagt gaaccaggtg ccaacgcctc caatatcaga
     3421 cttctcagta accaataatt tcacgttgct cgatgttaca aacttccata acccgcaaag
     3481 cgccaattat ccagggaaga acctggtgac aatgtggggc gaagttacat ctggaacgca
     3541 ggtctctacg acgctgacca gcacgatcgg tcctgatacc ggcgcgtcca tcctgggttg
     3601 ctggattgaa gcatccactt cccaaacaca ggcgcagatc caggtgcagc cgagtcaaat
     3661 caccttttct gacgcagttg taggaacaac tacaactgcg caaggcgtca cagtctcaaa
     3721 tacaggaaac gcaagcgcat ctctgcaatc ttcttcagtg agcggcgatt ttcaaatcag
     3781 cggcagcact tgtggaactg cactagcgcc gaacaccagt tgtaccgttt ctgtggcctt
     3841 tacgccgaag gcctctggat cgcggagcgg atctttaagt attcaggaca atcttgggac
     3901 acatctcgca cagcttaccg gacgaggtct gacgcaggcg actgccgacc tgtctgcctc
     3961 tggattgaac ttctttctgc cagtggtggt agggcagaag agcgccgccg aactcattac
     4021 tcttgctaac aatggtgacg ctgtcctggg acaaatcgct gtaagcattt ccggcgactt
     4081 tacgatggat aatgactgcg gcacgcagct ggcaggacac acctcctgcc ctattctagt
     4141 gacttttgca cccacacaag ctggagtgga gcaaggggtt cttcaggtaa acaccgtctt
     4201 tggtgcgcag caagtccggc tttcaggaac aggcatgtcg cctccggcgc tcttcctggc
     4261 ttccactctt cctggatttg gaagccaggg tattggcaca gccagcagca cgcaattcat
     4321 tacagtcgtc aatcgtggcg gctcaaccct taccggcctc accttttcca taagcggcga
     4381 tttcactatc tccaccaaca cttgtacacc tggcgctgcg ctaacatctg gaagcgcttg
     4441 cattcttggt gttgttttta ctcccacaca gacaggaaca cgcaacggaa cactcgccgt
     4501 ttcaggtgca aatcttgcat ctcctctgat cgagccgctt caaggtacag gaatcgattt
     4561 cgaattgaca atcctcggcc agacatccca gaccattcaa agcggacaat ctgctaagta
     4621 tcaggtgcag ttcacattta tcggaggacc tggcggagtg ccttacatca cctgctccgg
     4681 ccttccgaaa aacgccagtt gctcgataga tcaatcagca ggggcggtat ccacggcaaa
     4741 cgcacaaacc atcaacgcta cggtgaccat tgcacctgca gccacaactg cttctactcc
     4801 cgaaaagacg gggcttactt tggcattctt tctgccattt ttcgctggat ttgcaaggcg
     4861 acgcaatctg atgcgcgctg tgcttctgtg ctttgtttct gcaatgctgc tggcctcagc
     4921 cgcatgcggt atccagccta acagtttcag ccagacagcg gacgcgagcg gattacaggc
     4981 cggtgtgtac accatccata tcagtgcgtc gctcgacagt ctgacaaaaa ccgtacccgt
     5041 cacactgact gtcaaataac ggccattcac aggagccggc acatgaagtg tatgcttccg
     5101 gctgtcctct tgaaattatt tatatggaat cggcctgctg tgcatctttc acgcgcaagg
     5161 gtgggatatt catttcgcga atcttgtaca tcagcgagcg atagctgatt ttcagtacgc
     5221 gcgctgcctt cttacggttc cagttgttgg acgcgagcac ctgcaaaatc aatctgcgct
     5281 ccaggtcctg cacggtctgc cttgtgatgc gcttgagtga gaagtcgcca tcaaaggatg
     5341 tggttacagc gaaaaatgga gcattgccgc gtgtaagatc ggcaagcaca gcatcttcct
     5401 gtccaaaaat gacatatctg cggacaatat tctccagttc gcgaatattt ccgtaccatg
     5461 gatagtccat gagtgcctgc acggcagagg aagagagcgg acgcgccatg caattcagat
     5521 gttcgctgtg gtgctctatc agaaactcga caatgcgcgg aatatcattt ctgcgttcgc
     5581 gtaagggtgg cagggaaatg gtcactccgt cgaggcgata taagaggtct tcgcggaacg
     5641 atcctgctgc aatgttttct tccaggtttt ggtttgttgc ggaaacaatg cggacgtcca
     5701 cgtgctgttc cgtttcgccg cctattctgc agaaccttcc gtcctgaagc acttgcagca
     5761 gtttggcctg cagattgggt tccaggtctc caatctcatc cagaaacaaa gtcccggcac
     5821 gcgcgctttc gacctttccg ggtttgctgg catacgcgcc tgtgaatgcg ccgcgctcat
     5881 agccaaacaa ttcgctttcc atcaacgttc caggaattgc ggggcaattc accttgacga
     5941 atggaccgtg cttccacggc gaaagatggt gcaacagctt cgcaatcacc tctttgcctg
     6001 ttccactttc tccgcgaatg agcacgggaa tatttctgcc agctgcgcgc tccagtttaa
     6061 gctgcacaat cttcatggca gcggttgtgc caaatatcac atccggtggc ggaagattga
     6121 tttcgatcgt atgtggtgat tttgttcccg tgctgaaaac cataagctcg tgtatttact
     6181 tgcatttttc gaaccaaata ccatgcttcg gggtgggatt acacaagaaa aacagcgagt
     6241 aattatgaga agaacttctc tatgtagttg ctttgtctaa agttgcaaca caatgcagac
     6301 aaatgtttgt gtaaaaactt tttcatttga catgcaaatc tttgcctttg tatagttctt
     6361 tctgtcgtcg tactttcccc ctgtttattt tggcactgga gcatacatct taagtgtgtc
     6421 cggcagccct gaagtctcct ccctgcaagt aacgttgttt aacctggaac cactatctgg
     6481 cgagcattcg tgtcaagctc tcagtatggt gtgcccttaa acaacattca ggaagacgcc
     6541 gcatattgct tccatgagaa cggaatttga aatcgccttg gagaagttat gacaaccgca
     6601 caatcattgt ctgctgtaag tcaggaaacg gatctgtctg ctaaatcagg actagaggga
     6661 gcgcaaatct tcttaattga ccaggccgga gttatggagc accttcgctc actgctttcc
     6721 ggtcattcat gcagagtcac tggtgtttca agaagcgaag atgcaatggc aaagctgaaa
     6781 accagtcagc gcccgaatgt gatattgatt gatgcccatc ttccggggcc aggtggattg
     6841 tcagtgttgt cggcggtgcg cgaaatgtat cccagtgtgc cttgtgttat gttgtcatgc
     6901 tcttacgatc cacggctgat tgtcgaggcc atgaaacgcg gcgcagtgga tatcatcctg
     6961 aaacctttcc ataagaatga tgttttggaa acgctgcggc gctgcatcgc cattgtggac
     7021 agctcgtatg aaaaccaggc acgtgaaatt cccctagtag acaacacatc gttcgtcttt
     7081 gccagtgaac gcatgcggac gattttctcg cagtgctcca cgattgcccg ggtggacctg
     7141 ccagtgctaa tccttggcga gagcggcaca ggcaaggaag tcctcgcaca gttcattcac
     7201 aagatgtcgc ctgcttcgca ccgcaccttt ttgaaagtca actgtgccgc catgccggct
     7261 gatcttctgg aaagcgaact gtttggatac gagcagggcg catttaccgg cgcagtgaaa
     7321 gcaaagcccg gaaaatttga gatgtgcaac aacggcacga tctttctgga tgaaattgga
     7381 gaaatgccat tcccgctcca ggcaaagctg cttcatgttt tgcaggacgg cagtttttcg
     7441 cgcctgggtg gacgcacaac gatgaaggtg aacgttcgca tgatcgcagc caccaatatt
     7501 gatatgaagg ttgctattgc agaacgccgc ttccgggaag accttttcta tcgcctgaac
     7561 ggattttctc tgcaccttcc gcctttgcgg gagcgtaagg atgaaattcc tgtccttgtc
     7621 cggcatttcg tggcgaagct ttctgacaaa tatgctcaga agtgcccgac tgtttctgac
     7681 gagatgctgc gtgcctgcat gaactatgca tggcctggaa atattcgcga gctggaaaat
     7741 tttgtgaagc gctttcttgt gctcggcgat gaacggatga tgatggccga gctgcgtgga
     7801 gaccgtaaac ttcccgcggc cagtggtatc ggtgggcaac ctgagccagc agctatgagc
     7861 ctgaagaagc tcgttcgcag ggtgaaggct gatgccgagg ctgaagccat cagtgctgct
     7921 cttgaccgaa acaactggag cagaaggcag gcggcagcag aactgaaaat cagctataag
     7981 gcgctcttgt ataagatgcg cgactatggc ctgtcagaac ctcaggctat gagggcataa
     8041 gggtggagtt tgatttggaa gaggcagcat tcgtggatgt gaaagaagcg aaatctacca
     8101 gaaaagaaga agaaaaagtt gtagtccatc tgacaggcgg aagaatcata aaagggtttc
     8161 tgcctgtcga aagtccgaaa gacctgcaat cgctgcttga gaagccgcgc ctgaatttct
     8221 cagggaccat tacggtcttt tctgccgaag gccgtactcc ggtagaaatt gacgcgcagc
     8281 agcttaaggc catcttcttt gtgaactctt tttccggcaa tagtgaacgg aatgctttgc
     8341 ggtattacat tcacgggccg gaagtaggcg ctatctgggt tgaggtgcag ttcaaggacg
     8401 atgaaattat ggaaggtatt gtccataatt ccattcatca tttaattgca gacggtttct
     8461 ttctgcaccc ctctgacccg ggcagcaata acaaactggt gtatatccac aagaacgcca
     8521 ttaaagatta ccgggtgctg ggcgttcgca cattagcaaa cggataatgc gaggcggccc
     8581 ttgccagagg cgccgcttcg tcaatttcca tttcaacact gtagttgcag tctgcttgtt
     8641 tgtgcacctg aagaacgttc cctgttccag caatattcaa tgttggttcc agagtaattt
     8701 ctggcgggac actcaataca aacacaacgt ttcccggccg gacctcaaga ttcgtgtgaa
     8761 agaagatctt tctgccgtgg atctcttcga taacgccctc tatgacagac gggctgtcag
     8821 gacatcgaat ttctattggc atcctggctg gaaataatgc ggggctatgc tcctgcacaa
     8881 ggggcgaagt cactctcttt ctcctccgga tttaaactgg cactttggca gacagccctg
     8941 cagacccgaa aatggcttcg ggtacgaagt tttcttcctg gttggtaata gattttccat
     9001 tctgattctt gcttctgggg catgttccgt acttttgttt gcacaggtat ttccatcccc
     9061 tttgtgcctg ttttattgca agtagatggc cgaattgcca atctgaggaa aaaggcgcag
     9121 tgagaagcaa tgcacccggc agccaaagct tgaaaagcaa actttctccc actattaaga
     9181 aaagattttc ctctcccggc aacctgcgcg gttttgtccg gaaccggacg cgttgctttt
     9241 ctcaacggca gggccggcaa ggacattaag cccgggactt ctttcagcgt aagcagggtt
     9301 attgcgacaa aaccggcctt tcttttcgcc ctctggaagc caaattgctt gttaactggc
     9361 agcaggtcga attatgtgtg gcattgctgg aatcataagt caggactcta ccgcgcttcg
     9421 gacgcttgcg cttgagcgca tgacagaggc cctgtctcat cgtggtcccg acgaacacgg
     9481 gttgcatatt gaaggaagcg ctgctcttgg ccacgcccga ctcagcatta ttgacctggc
     9541 aggtggccac cagccgatgc agcttcatga cggccgcatg tggaccacct tcaacggaga
     9601 aatttttaat tacatcgagt taaggcagga gttagagcaa aagggccata agtttgttac
     9661 aaagtctgac acggaagtta tcctccatct ttatcaggaa tacggcacag actttgtcca
     9721 gcgcatgaac gggcagtggg ccctggctat ttgggacgac gcacgtaagc agttatttct
     9781 ctcccgtgac cgcatgggca ttcgcccgct ctattacaca accgtgggtg gcgcacttat
     9841 tttcgcctcg gagattaagg ccatcctcgc tcatcctcgt gttccgcgcc aacttgattt
     9901 gcaggcgctt gaccagatat tcaccttctg gtgcccgctg ccttcgcgca ctaccttcaa
     9961 aaatatccac cagcttcctc cagggcattc cggcatcttc aaggatggac acctgaaaac
    10021 atggcggtac tggaagctca attacgaatg gcagacagat gagtctccgc gtgctgtcga
    10081 acgtgctgct gatcaacttt ttgaattgct tctcgatgct gcgcgcattc gacttcgtgc
    10141 cgatgttcct gtaggcgcat atctcagcgg aggactggat tctgcagtca ccactgcgct
    10201 tattaccagg ctcgttcctg aaaatctgcg cacattctcc gttggatttg aggatgcgga
    10261 cctggacgaa agcgtctggc agcgcgaagc agcttctttc ttcggggtgg gccacgagag
    10321 catccgctgc tcggcagaag acatcggcga agtctttccc gatgtgatct ggcacatgga
    10381 gcagcctgtt ctgcgcactg cgcctgctcc tttatatata ttgtcgcgcc tggtgcgcga
    10441 aagcgggttc aaagttgtcc tgactggaga aggttctgat gagatactcg gcgggtatga
    10501 catcttcaag gaagcgaaag tccgccgctt tatcgccaga aacattgatt caaagtttcg
    10561 cccgctcctt ctcaggagac tctatccata tataggtaat atccagaagc aatcgcctgc
    10621 atatctgcag cgcttcttcc acgtaaatcc atcggacgtg gaaaatccgt ttttttcgca
    10681 tattccgcgc tggacgctga ccgcacgcgc gaagacgttt ttcagtgatg atgtgcgtca
    10741 ggagcttggc ggctatgact gcctctccga catgcggtca gaattgccaa cagaattttc
    10801 gcgatgggac ggttttagcc aggcgcagta tctggagacg gcgcacttgc tgccaaacta
    10861 tatcctttca gcgcagggag acagagtggc aatggcgcat tccgtcgagg gccgctatcc
    10921 atttcttgat tacagagtgg tcgagtttgc atcgcatctt tcacctgcgc tcaagatgaa
    10981 agcgcttgat gaaaagtatc ttctaaagcg catggcgaag ggcctggttc ctgaatccat
    11041 tctgtcgcgg cccaagcagc cgtatcgcgc accggacggc cggagcttcg ttcatggaaa
    11101 agcgcgcgag tatgtggacg aaatcctgtc tgcggaaaca atacggaacg caggcatctt
    11161 caagccagag ccggtcagga tgctattaga gaaattccgt tccgggcggc ctacaaatac
    11221 tggagacgat atggctttag ttggaattct ctccacatcg ctcctgatgg accaatttat
    11281 ttatcaaaat cgaaagcagg actgtgcatg tacaacctac agcgagagct tcgcgacttt
    11341 atcgtaacta acttcatgtt cggcgacggc gccggattgg cggacaacga ttccttcatc
    11401 aataaggggg tcattgattc cactggggtc ctcgaacttg tgatgcatct cgaagagacc
    11461 tatcggatcc aggtgaaaga caacgaactt gtccctgaaa atctggactc aattgataac
    11521 cttgttgcct ttatttctcg caagctcgcg gcggagcagg aacttgtcac cgctgcgcag
    11581 gcatagatgg agaagaacgt ggaccaaacc gaaaacctag ccgtgctgag tcatccactc
    11641 agtctcgatg ttgaagcaga ggcgcaacgc attgaagcct ccatccgcga gatggtcttc
    11701 aagcgattga agcgcaaagg cgcagtcatc ggcgtctccg ggggcattga cagcagcgta
    11761 gtggcgtttc tatgcgcgcg ggcgcttggc cccgaacgcg tcatccttct ctttactccg
    11821 gaactcgatt catcgagcga gaacctgcgc ctgggtcaaa gtgtcgcgga cgctctcggt
    11881 gtgcggtctt ttctggagga catcagcccc gttttgaaag gggcaaggtg ctacgagcgg
    11941 cgtgatgaag ccatccgcca ggtctttcct gagtatggtc ccggctacaa gatgaagatc
    12001 gtcctgcctt ctattgatga agacagcaag tattcactct tctcgctggt ggtacaggcg
    12061 ccggatgggg cggtccataa gagaaggctt acggctgacg cctatctcgg cattcttgca
    12121 tccaccaatt ttaaacagcg cgcccgcaag atgatggagt atcactatgc tgaccgctat
    12181 cagttcgcag ttgccgggac cccaaaccgt ctggaatatg accagggctt ttttgtgaag
    12241 aacggcgatg gatctgccga ttttaaaccg attgctcact tatataagtc ccaggtctat
    12301 cagttagcag agtacctagg cgttccggag gaaatacgca ggcgtcctcc tacaaccgat
    12361 acctattcac tcgagcagac gcaggaagaa ttttatttct ccatgccgtt gcaacagttt
    12421 gatctctgcc ttttcggaaa aaacaacggg cttgctcccg cagaaatagc agacctggct
    12481 gggttgccgg cccatgcagt ggaacgggcc tatcaacaaa ttgaatcaaa acgtgcagta
    12541 actcactacc ttcggatgaa gcctttaatg gttgagcctg ttacagagtg cgagatcaag
    12601 gaataagaga ggatttataa attgtcactc gcaactgtta gtccagaagc catagtgcgg
    12661 aaagaagagt atggatcgaa tgtctatgac tatcttctcg ccggtaagga cccggaacgg
    12721 gtggcgctca ttctgctcga tggcgaaaag acctatgaag acattctttt cagctcgcag
    12781 ttgattgcgt ctcatctgtt gcaacaaggg cacagcaaag gagaccgcgt tcttctcatc
    12841 gctgaaaaca gcttcttctg ggtcgcgagc tacctgggca ttctgcgtgc aggattagta
    12901 tgtgtaccgt tgcctgctaa tactgcgcag gaaacagttt cttatattgc tgagatcaca
    12961 gaagcaaagt ctgcatgtgt tccggcaaga gtgtttttaa aaaataaact gcggttacct
    13021 gtcccggtgc tcaccgatgt gccagtacag gaggcagaga aaccaggagc acaacatcgt
    13081 aacggcttca tccagcctca gaattttccg cagatctcgc gcgacgatct cgctgcgttg
    13141 atgttcacca gtggaactac aggaaagccg cggggagtga tggttacgca tggcaatatc
    13201 attgcgaata ccaactcgat cattgaatat cttcgcttga gcagccggga ccgcattatg
    13261 gcagtgctgc ctttccagta ttgttttggg gcttctctgc tgcacacgca tttgcgagca
    13321 ggcggaagcc tggttgtaga tccacgcttt atgtttccgg agaaaatcct ggagcgcatg
    13381 cgtgagacgg aatgcaccgg atttgccggc gtgccgagcc actaccagat attgctccgc
    13441 aagtccgcgc ttaaggacta ttgtttcccg cacctgcggt atgtccagca ggccggagga
    13501 catctggccc cggcattgat ccgggaattg cgtgaggcgc ttccgggcac agaaatcttc
    13561 gtcatgtatg gccagaccga ggccacggcg cggctttctt acatgccgcc ggaaatgctc
    13621 gacagaaagc ccggctcgat tggcaagggc gttccagggg taaagctgcg tgtgatttac
    13681 gctggccgcg atgctgctcc tggcgaaatc ggggaaattg tcgcacaggg agagaacatt
    13741 acacagggct attggcgcgc tgaagaagag acagcaaccg ttttccgtga cggaatgcta
    13801 tacaccggtg atctggccac ggtagatgaa gatggttata tctttatcgt ggaccgggcc
    13861 aaagacttca tcaaatgcgg cggcaaccgg gtaagctgcc ggcaaattga agaaaagctc
    13921 ctcgaatttg gcgacctgct tgaagcagct gttatcggtg tgcctgatga aatcctggga
    13981 gaagcggtca aggcatttgt tgtaccccgc ccaggagtcg tttcggacct tgaaccactg
    14041 ttgcgccagt tctgcacacg aaacatgttg cctgcccagg tcccgagaga atttgtcttc
    14101 cttgatgcct tgcccaagaa tgcctcagga aaggtggcaa aatctgtact gcgcgagatc
    14161 aggatggcat catgacgcgc aagctggcag ttgcgctcac tctggtattt cttgcaatct
    14221 cttcctgcac aaaaaaggaa catccatcgc aaatgatttc agaatcacaa tggagcagga
    14281 tcgaaggcct gcgcatctat tttggacacc agtcggtcgg cgaaaacatc cttgatggta
    14341 tccgcgatat tgagaagcaa tcgcaccgga acagcctgcg tattgtttcg agtgacgatc
    14401 cgtcgagcat cgaaggcccg gccctgatcg agtcccatgt gggacgcaac ggcgacccca
    14461 catcgaaaaa tattgccttt cgtgacattc tcgataaggg ctttggggca cagggcggta
    14521 ttgcgctgta taaatattgc tttgtggaca tacggccaga gacggacgtc gccaaaatgt
    14581 ttgcggaata ccgtgacaac atgcgcgatc ttcgctcccg ttatccgcgg ttgacgattg
    14641 tccatgtcac cattccgctt acaactgtcg agccaggatg gaaggcagtt ttgaaaaatc
    14701 tgctggggcg gcccaccgag cgtgccattg cagaaaagcg gcaccagttc aatcaactgc
    14761 tgcggcaaga atatgcaggc aaggagccaa ttttcgatct tgcagaggcc gagtctactc
    14821 tgccggatgg ctcacgcgca tatttcatct cccggggcga gaggatatac acgcttgcac
    14881 cggagtacac ctatgatggc ggtcatctga acgaaagtgg acgaaacgta acggcccttc
    14941 ggcttttaca gaccctggcg caattttagt ttgtcttcaa gtgagtgttg cttttgaaaa
    15001 ggaagagaac agcctttctt tctcttcgag cctttagcca tgcaatgccg ccagggtctt
    15061 ttccaggctt gctgcatctt ctacgttcag tgctgtctgg gtgcgatcta tctggcgcag
    15121 caggccgcag agcacctttc ccggaccgac ttcaataaag tgggtaggct tttgctcgat
    15181 cagcagacgc atggattcga cccagcggac agagcctgtg acctgacgga tgagggcgtc
    15241 gcgcagagcc tctgcgccgg tgacaagtgc cgcatccaca tttgtgatga cagggacttt
    15301 gggcgcggag aaattcaggc gccgcaggtc ttcagccaag cggtcctgcg cgggctgcat
    15361 cagcgcgcaa tggaagggcg cgctcacagg aagcatcacg gcgcgcctgg ctccagcctc
    15421 tttggcgagg gcggcggcac gctctacggc agcagcagta cctgagatca ccgtttgctc
    15481 aggcgaattg aggttcgcgg cggaaacaac tgcttcggtc tctgcggcgg cctttgcaca
    15541 aacctctatg actgcgtcag agctgaggcc cagaatcgcg gccatcgcgc cctgtcctgc
    15601 ggggacagct tcctgcatgt agcgtccgcg attgcgcacg gtctttactg catcggcaaa
    15661 ggaaagcacg cccgcggcaa cgttggcgga gtactcgccc agcgagtgtc cggcggcaaa
    15721 gccgggagtg atgccctttt ctgccagcac gcgctgcgcg gcgatggaaa cagtgaagat
    15781 ggccggctgg gtgaactcgg tcaggcgaag ctgctcctcg gggccttcaa agcagagttt
    15841 tgagagcgaa aagccgaggg cttcatcggc ttcctcaaag gtctgtttgg cgactgggaa
    15901 tttgtcatag agatcgcggc ccattccgac ggcctgcgag ccttgtccag gaaaaagaaa
    15961 tgccagcttt ttgtcactca tgtcataggt atttcaaatg attccaggcc tgaggcgcaa
    16021 ctgaatgaga tttcgctggc agagatacgc agtgcgctca ctcagtgcct attgcggatt
    16081 tggctttaaa ggggaaggcc ccgccctcat tttggagaag cggggccatc gttttgcaag
    16141 cagcctgtaa gccgaattct gtcgaggacg gtcattcctc caggcgacgc attgctgcgg
    16201 cgctcaagcg acctacccgg aggtttccgt tccacttaac ggcttgtgcc gccagtctgg
    16261 atgaccctcg ttggcgcgcc gggccggcac gccgcgcggc gaaacgggtc ttgccgcgca
    16321 tccctcccta tttggtcttg ctccgtgtgg ggtttaccct gccttcggcg ttaccgtcga
    16381 agcggtgcgc tcttaccgca ccttttcacc cttacctttc gccttgcggc ggaggcggta
    16441 tgttttctgt ggcactggcc gtcttcaggc cttgaggcct gaatcccgga cgttatccgg
    16501 cacactgccc tgcggagttc ggactttcct ctcctcagga acggcgcg
//
LOCUS       DASRBV010000016         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_405887, whole genome shotgun sequence.
ACCESSION   DASRBV010000016 DASRBV010000000
VERSION     DASRBV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405887"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFA02_00490"
     CDS             complement(<1..191)
                     /locus_tag="VFA02_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HZU08351.1"
                     /translation="MTMQETFLQKAYQAAAAAGHIFPEYAACEAALESGWGLSHLATE
                     ANNLFGQKQAHPPLPGTET"
     gene            complement(778..1131)
                     /locus_tag="VFA02_00495"
     CDS             complement(778..1131)
                     /locus_tag="VFA02_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08352.1"
                     /translation="MSAPSILHVCSRDLIRVLRDEILRLQGYHVTSTLSIEEAEALYK
                     QSKYDLVLVDVEGDGRIPLAEKLCDDIKKMDPEQKVAFVCNYRVSKASDCPDEIIHSE
                     FNPKALVEGIRDALA"
     gene            complement(1299..>3488)
                     /locus_tag="VFA02_00500"
     CDS             complement(1299..>3488)
                     /locus_tag="VFA02_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HZU08353.1"
                     /translation="LEDGEKRTSKVLPMAKRYGAAVVALTIDEQGMALTADKKIAIAK
                     RIYDLATKKYGLRGIDLIFDPLTLPISTGQEEYRTAGIETLEAVRRIKQELPEAKTIL
                     GVSNISFGLNTYARRVLNSVFLKEAVDRGLDAAIVNYSKIYPLYKIPEREVELARKLI
                     FFDQSEGDPLQSYMAHFSGLDKNATATEEVSLESLSIEDKLKQLIIRGERAIGQGAQK
                     QTLEAILEEALQTYTPLDLINTVLLDGMKTVGDLFGARKMQLPSVLDSAATMKAAVAY
                     LEPKMEKVEGTQKGTIVLATVKGDVHDIGKNLVDIILSNNGFKVVNLGIKQPADVIIK
                     AAQEHNADAIGLSGLLVKSTLEMKYVIQDLERLAMETPVICGGAALTRKYVEDDLRRE
                     YSSAVFYAEDAFAGLHVMTDLTTEDEEARKKRLEDGRTVKVFTKVTVAQEAPATTEVS
                     TTRSTWVSDAPDIPKPPFYGVRVRKDFCLDEVFSYINETALFKNQWQLKTASATDYIH
                     LVEEKYRPILEELKAEIKAKDWFHPKLVYGFFPAQADGNDLVVYEPADPATGTPKERL
                     RITFPRQREGRQLSIADFFLPKDSGKFDVVGFSVVTIGDRASQVTKELFESGDFTRYL
                     YVHGLSVETAEALAELAHKLIREELGIVGEDSSQIKDLFHQKYRGSRYSFGYPACPNL
                     EDQTKIFELLEPEKNIGVRLTEGFHLEPEQSTNAIIVHHPQAKYFVV"
BASE COUNT          646 a          871 c         1093 g          878 t
ORIGIN      
        1 atggtttccg tgcccggaag cggcgggtgg gcctgcttct gtccaaagag attgttggcc
       61 tcggtggcga gatgggagag tccccatccg gattcgaggg cggcttcgca ggcggcgtat
      121 tccgggaaga tgtgtccggc ggccgcagcg gcctggtatg ccttctgaag aaatgtttcc
      181 tgcatagtca tggtttttcc tccaaatgga aagagcggag cctgcggctc cgcccggttg
      241 tggggtacac cctacttagt tttttgtaaa ttcttgcaaa tacttgggtt actggttggt
      301 ggtttctaca gcattctcac agatcgtctg aaccatgccc gaaactacag tcgccgacac
      361 tgcaccgggc aaatggagtc tacggaatct gtgaaaatgc cgtaaggccg cgatccgcag
      421 agtggtgttg tgccaattct gcttatgccg gtcttccgac gcttttggaa aatcttctgg
      481 cccagggatc ccgggggacc taaatttctc aaattcttga aaagaataga cttgtctgcc
      541 tgtttgaagg gcttcgtttc actgtgattt acgaaaaggc tgatttgtca ttccggacgg
      601 aggcagaaga ttctttctct cttcggtcgc cagaatgacg ggaccctaca taagacactg
      661 caaacccaca tcttttcagg gtgtggggca ttccggctta ttctgcccct tatttttatt
      721 atttcagatt ggcggggtgc ttttggtatt tattcgttat aacgaataaa atttttttca
      781 agcgagggcg tccctgatcc cttcgacaag ggctttcggg ttgaattcgc tgtggatgat
      841 ctcatcgggg cagtcgctgg ctttggacac ccggtaattg cagacaaagg cgaccttctg
      901 ctccgggtcc attttcttaa tatcgtcgca gagcttttca gcgagaggaa tccgcccatc
      961 tccttccaca tccaccagca ccagatcgta cttggattgc ttatagagcg cctcagcttc
     1021 ttcgatggag agggtagagg tcacgtggta tccctggagg cggaggattt cgtcgcgcag
     1081 cacgcggatg aggtcgcggc tgcaaacatg caggatgctg ggggccgaca tacagcgaga
     1141 gcatagcaga atcctgcgat attggcactg ggtcttccat cccagtgtcc ggagttaccc
     1201 agccccgggg cagggctttg tatccttcac ttccccttcg ctccgctcag ggtccggcgt
     1261 tgccatctac aggatgacta tttgttttaa atcactgctt acactacgaa gtacttggct
     1321 tgcgggtggt ggacgatgat ggcgttggtg ctttgttcgg gctccaggtg gaagccttcg
     1381 gtcaggcgga cgccgatgtt cttttccggc tccagcagct cgaagatttt ggtctggtct
     1441 tcgaggttgg ggcaggccgg gtatccaaag gagtagcgcg agccgcggta tttctggtgg
     1501 aagaggtctt tgatctggga agagtcttcg ccgacgatgc ccagctcttc acgaatgagc
     1561 ttgtgggcaa gttcggccag agcttcagca gtctcgaccg aaaggccgtg gacataaagg
     1621 tagcgggtga agtcaccgga ctcaaacaac tctttggtga cctggctggc gcgatctcca
     1681 atcgtgacga cagagaagcc gaccacatca aatttacccg agtctttggg cagaaagaag
     1741 tcggcaatgg agagctggcg gccttcgcgc tggcgcggga aggtgatgcg caggcgctct
     1801 ttgggcgtgc cggtggccgg gtctgccggt tcgtagacga ccagatcgtt gccgtcggcc
     1861 tgcgccggaa agaacccgta gacgagtttg ggatggaacc agtctttggc tttgatttcg
     1921 gccttcagtt cttcgagaat aggccggtat ttttcttcga caaggtggat gtagtcggtc
     1981 gcggaggcgg tcttcaactg ccattgattt ttgaagagcg ctgtttcgtt gatataggag
     2041 aagacttcat cgagacagaa atccttcctg acgcgcacac cgtaaaaggg cggtttcggg
     2101 atgtccggcg catcgctgac ccacgtggag cgcgtggtac taacttccgt ggtggctggc
     2161 gcttcttgcg cgacggtgac tttggtgaag actttgacgg tcctgccgtc ttcgaggcgc
     2221 tttttgcggg cctcttcgtc ctcggtggtg aggtcggtca tgacgtggag gccggcgaag
     2281 gcgtcttccg catagaagac agcggaagag tattcgcggc gcaggtcgtc ttcgacatac
     2341 ttgcgcgtga gcgcggcgcc gccgcagatg acgggcgttt ccatggcgag gcgctcgagg
     2401 tcctggatga cgtacttcat ctcaagggtg gacttgacga gaaggccgct gaggccgatg
     2461 gcatcggcgt tgtgctcctg cgcggccttg atgatgacgt cggcaggctg cttgatgccg
     2521 aggttcacca ccttgaagcc gttgttggag aggatgatat ccacgaggtt tttgccgatg
     2581 tcgtggacgt cgcccttgac ggtggcgagc acgatggtgc ccttctgcgt gccctcgacc
     2641 ttctccatct tcggctcaag gtaggcgacg gcagccttca tggtggctgc tgaatcgagc
     2701 acggaaggaa gctgcatttt gcgcgcgcca aagaggtcgc cgacagtctt catgccatca
     2761 agcaggacgg tgttgataag gtcgagcggc gtgtaggtct ggagcgcttc ttcaaggatt
     2821 gcttcgagcg tctgcttctg cgcgccctgg ccgatggcgc gttcgccgcg aatgatgagc
     2881 tgcttgagct tgtcttcgat gctcagcgat tcgagcgaaa cttcttccgt tgcggtggcg
     2941 ttcttgtcca gaccgctgaa gtgcgccatg taggactgaa gcgggtcgcc ttcagactgg
     3001 tcaaagaaga tgagcttgcg ggcgagttcg acttcgcgct cagggatttt gtagagcgga
     3061 tagattttgg agtagttgac gatggcggcg tcgagtccgc ggtctacggc ttctttgagg
     3121 aagacgctgt tcaacacgcg gcgggcgtag gtgttgaggc cgaagctgat gttgctgaca
     3181 ccgagaatgg tcttggcctc tggcagctcc tgcttgatgc gccggacggc ttcgagagtt
     3241 tcgattcccg cggtgcgata ctcttcctgt ccggtggaga tgggcagggt gagcgggtcg
     3301 aagatgaggt caatgccgcg gaggccgtat ttctttgtcg ccaggtcata aatgcgcttt
     3361 gcgatggcga tttttttgtc agccgtgagc gccatgcctt gttcatcaat ggtgagggca
     3421 acgacggccg cgccatagcg cttcgccatg gggagcactt tgctggtgcg cttttcgcca
     3481 tcttcaag
//
LOCUS       DASRBV010000017        25748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_355260, whole genome shotgun sequence.
ACCESSION   DASRBV010000017 DASRBV010000000
VERSION     DASRBV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 25748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25748
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355260"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFA02_00505"
     CDS             <1..347
                     /locus_tag="VFA02_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08354.1"
                     /translation="HQLYISLYEELRGDYAGWLSRLLLFLGVNPAFVPAHSEVPSTPH
                     LLKLRGLQQFRHLGNRIIPGRIRTRIKKHVYQERLPELHAEDQAILVRYYRDNILKLQ
                     DLIQKDLTSWLH"
     gene            complement(356..1507)
                     /locus_tag="VFA02_00510"
     CDS             complement(356..1507)
                     /locus_tag="VFA02_00510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HZU08355.1"
                     /translation="MKFRSKHLRHPILAARMAMRLVAGYLDRAILAYRGAWRFRKDPR
                     YSGQTVWKGFAPRTSHASGDNALFERICAAYASAMEHQHTALEVYGPTQWWENLRQSA
                     LRPAMQALSTQNLDALRGMYGNFFRDNCSAGLVGRPCGLPFEKFCRAASSIHWRAYLA
                     DELFRLEYWARQTESRCSLHELAGPEIGNPFGIWLDDVLVQTSSAYHHYCAWKISHLL
                     RGGRAVVAEIGGGFGGMAYYLFRGRKDITYINFDLPESLALATYYLAKAFPEMQLLLY
                     GEDKQGVCPDIIMMPVTELDKMPADCADITFCSHVMSDLPEVAAGVYLNNVSRMTRYA
                     FLCVMENRVANIAAPRDFQIAEKVPAYWNSRGKRIASEMEYLFLRKKPT"
     gene            complement(1514..1744)
                     /locus_tag="VFA02_00515"
     CDS             complement(1514..1744)
                     /locus_tag="VFA02_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU08356.1"
                     /translation="MSAAQPRSDRHEARPKSVHEPQKSEGERVFDENCSRCHNAPEGF
                     SPRISGTIVRHMRVRANLSEKDEQALLRFLNP"
     gene            complement(1819..2862)
                     /locus_tag="VFA02_00520"
     CDS             complement(1819..2862)
                     /locus_tag="VFA02_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08357.1"
                     /translation="MKEPYIERMLRSFVSAMSFLFVMGFASVFYAQNTPLISGGMGFE
                     TSTNGGNTSYTPVIMPLLAAPLGDHLLVESRAILLDTVFPKTGKGYDSQHFIALSYLQ
                     GDYIATPHVTVVGGYFLIPFGTYNDRLSPIWISNFQDAPLISSLGTMTSGSATGGMLR
                     GNAWSTSKVSIDYAAYFSAGSTNEQFQSSRASGGRVNAYFPHARLEIGTSYGHLLEHK
                     QENFYGAHVWWEPAQVPLRVRSEYAHGEHSQGLWIEGDYRLSQFGGAESLIGRLEPVF
                     RWQQTFRNSPDATDGLPSVDTKRADFGLDYHLPHEVRINTSYARQFSSTGNRNIWETA
                     IVYRFLFPAWRGK"
     gene            complement(2876..3931)
                     /locus_tag="VFA02_00525"
     CDS             complement(2876..3931)
                     /locus_tag="VFA02_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08358.1"
                     /translation="MSTLVQITPAISGKKLQQHRIMQLPVLVLFPHNRCNCRCMMCDI
                     WRIRETRQLWRSDLAPHMESIRALGVRWVVLSGGEPLLHEELGGLLSLFRAENIRTTL
                     LSTGLLLKSCAQMVGSSVDDVIVSLDGPPAVHNTIRRVPDAYQRLAEGISAVRRLHPQ
                     IEIHARCTVQKRNHDALEATIEAAMVLGLNSISFLAADTTSSAFNRPDGWSDTRKSSV
                     QLNAEEIETLTVVVEKIIARYSGRFVREGPEKLRRIVEHFRAALGQQEPVAPRCNAPW
                     VSAILEADGTVRPCFFHQAIGNLDEAPLERILNSKRALEFRASLQINENPTCRNCVCS
                     LHYNEIDDNPMTKSETT"
     gene            complement(3928..5106)
                     /locus_tag="VFA02_00530"
     CDS             complement(3928..5106)
                     /locus_tag="VFA02_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU08359.1"
                     /translation="MKSRHPCLLLAGGRIAIDAQEAVHTDLSVCDSRISHATQTANII
                     RLDGYIVLPGLINAHDHLEFGMFPRLGSGPYPNWEAWAQDIYRPDGPPVRDLLAVPKH
                     LRLWSGGLRNLLCGATTVAEHNPYHAEIFTHDFPVKVLSRYGWAHSLKDEQTAKAAYH
                     QTPSEWPFFLHLAEGTDAAAAKEVDRLKQMVPLDDRLVLVHGIGIRRKQLHELSAAKV
                     WLVWCPSSNIFTLGKTFSAKTVRAYPYLLLGSDSPLSAVGDLLDELSFAHEHSGLSAE
                     ALYPMVTTRAAAALRLENGEGSIRAGAAADLIVLRDSGKSPAETLLWASRNDIQCVIR
                     DGALQFVSPVFSERVSAHLLDGFEESTWGGTTRLFPVSTCEKIREIQTYHEQIAEALV
                     "
     gene            complement(5103..5783)
                     /locus_tag="VFA02_00535"
     CDS             complement(5103..5783)
                     /locus_tag="VFA02_00535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU08360.1"
                     /translation="MITLSAVEAYQLWAPCYDAADNPVLALEERVLLPKIEELRGKHV
                     MDLGCGTGRWLRYAAECGAASLLGVDISPAMLSVAQRQCPPQVELICADVLSPLLKSA
                     SADVLAASFLLSYVPSLDAFVAQAARLLRTGGRLLISDLHPRARAHGWRSGFNVEGSS
                     YSIHTYPYSLDSLREGLYGAGLVQEFCKEPCFGRPERAIFERAGCEDMFDITSQQPAI
                     FVAGFAKK"
     gene            complement(5780..7243)
                     /locus_tag="VFA02_00540"
     CDS             complement(5780..7243)
                     /locus_tag="VFA02_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08361.1"
                     /translation="MIVLYYPRATRPANRRFPLSVLALAAVLEGREEYRIVDGNLDPD
                     PDKTIADLVQHHHVELLGVSVMPGPQMVAAMNTSKNIRTWFPQVPIVWGGYFPSIYPD
                     AALNAPYVDYIARGQGEVTLLELIEVVRGQRHVSSVAGLSYKTPDGRHHYNPERMMLG
                     PDVFPWSPFNSIPVEKYLRPSYFGKRTAAHQASVGCPFQCRFCGIGPAFGNRQKMESP
                     ARTEAILRHLVKEYRIDGVQFYDMNFFLREDHTRELMERLAPLGLQWWCEGRVDIVSR
                     YSDSTLALIRKAGCTMIFFGAESGSDWALKEMNKGITTEETLTVARRIRDFNIIPEFS
                     FVVGNPNDPERDTNETLNFVRRIKRLNPASEIIIQHYTPVPQREKMYGDIDGKIEFPT
                     TPEEWATPRWYNFTVRKDPATPWMRRRIRKLIDNFEVVVSCRWPTVQDIRAPRWGRVL
                     LKALSAWRYRFRIYAFPVELQLAHRWIQLRKPKVESI"
     gene            complement(7240..8229)
                     /locus_tag="VFA02_00545"
     CDS             complement(7240..8229)
                     /locus_tag="VFA02_00545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU08362.1"
                     /translation="MSTMVAKASVPPKAAMDDVLALLCPQCRRPSGSIDLFVSSQGPS
                     VLLCEHCGFVLEQKNGVWRALTFEQEREYAEFLRDYESIRLQEGRGSENSEFYRALPF
                     QDLTGNFSWQWKIRAKSYRVLEQKVLAKSDALRVLDLGAGNGWLSYRIALQGHTPVAV
                     DLSASAFDGLEAAHHYSEVLPCMFPRFQAAMDCLPFADEQFDLAIFNASFHYSADYSK
                     TLIETKRCLRHGAAIVIMDSPTYSKLESGMAMKRERHEAFCEKYGFSSDRMASQEFLT
                     PEIMDDLSRLGILWRKIQPWYGLSWAMRPWIAHWKGRREPSQFFLYIGYVVKS"
     gene            complement(8226..9020)
                     /locus_tag="VFA02_00550"
     CDS             complement(8226..9020)
                     /locus_tag="VFA02_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZU08363.1"
                     /translation="MTAAAFDAVAPAYDRIFTQSTIGILQRNQVWSVAREIFHPGDRI
                     LELGCGTGSDAVALPRLGASVVACDASPQMIAIAKQRAWEMGHSTHISFAVCANEDLH
                     KIAVDGEFDGVFSNFASLNCSRDLKAVVAGLAGKVRPGGKLLLCLFGRFCLWEVAWFL
                     SKGDAKKAFRRFCGSGASARVGGQEIQVCYPSVRRLKKIFSPEFSLIGWQGIGVAVPP
                     SYAESFFHGRPRLLSLLACLDENLRRMPGARAMGDHILLEFVRSAS"
     gene            complement(9017..10420)
                     /locus_tag="VFA02_00555"
     CDS             complement(9017..10420)
                     /locus_tag="VFA02_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08364.1"
                     /translation="MKAPELLLTHGYFLKEDPKELQIMKPYPPLGILYLCSHLRSKGH
                     AVEVFDSTFSTKAELIERLRTEQPSILGIYANLMTRSVVVQILQEAKQAGWKTVVGGP
                     EPGAYTDEYLEAGADIVVIGEGELTVEELLHAYRQSSSPDLSKIDGIAFRDSSGAVFR
                     TAPRAQIADIDAQPWPAREAINTAQYVETWRKHHGMGSISLITARGCPYQCQWCSHQV
                     FGHTHRRRKPHLVADELAWLRERYQPDMLWIADDVFTIHHGWLDAWAKELRERNLSLP
                     FECISRADRLNEKVIAALAELRCFRIWIGSESGSQRILDAMQRGVKIEAVRNAVALCR
                     QYGIQTGMFLMWGYEGEELEDIEATIDHVRSSNPDIFLTTVSYPIKGTPYYKNVASRL
                     VQIKPWAQTSDRELEITGRRSREFYKVADQLLRHEVELSRITNSSVQDKMTLLRRDEL
                     VRAVQQERKMLYAGGAA"
     gene            complement(10417..11883)
                     /locus_tag="VFA02_00560"
     CDS             complement(10417..11883)
                     /locus_tag="VFA02_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08365.1"
                     /translation="MSAMKHSAAVDVLCTHAYHLAFDRKQLERQQPYPPLGTMYAAAV
                     ARQHGFSVGLFDSMLDDPERGFAVALEEYRPRVVVIYEDSFNYLSKMCLGRMREVAWQ
                     MQAEASKRGIPVFIHGSDASDNAGVYLQQGFDAVLIGEGEQTLMELLNAVLHNGQVSE
                     WTVPGLAYRNPTGEIVRTPPRAPMKTIDHLPLPARDLVAMSRYASIWKTAHDRRSTNI
                     LTSRGCPFQCNWCAKPIFGNAFALRSAKSVVEEIELLRTEYGIEHLWFADDIFALDRH
                     WIQEFAALIEARECAIPYKMQSRASLMTPETVHALRRSGCEEVWMGAESGAQSVLNAM
                     DKGQTVENIVQAAHRLRDAGIRACFFLQFGYPGEGWKEIEQTIELVRRARPYDIGVSV
                     SYPLPNTVFYDRVKQQLGSKRNWVDSGDLSVLFQAAYSNEFYKALRDALHAEVDSWRE
                     LPSAKADIASLWSRVFNLEITCRNAAPTLLPVLCGGRA"
     gene            complement(11880..13391)
                     /locus_tag="VFA02_00565"
     CDS             complement(11880..13391)
                     /locus_tag="VFA02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08366.1"
                     /translation="MAKRPRRKIVFFFPSFSSSEATAPLGLLAVSTPLLRAGYEICLI
                     DSTITPNFKRRVLEEVKDALCLGISLVTGPMIRETVEIARAIKAWDPKFPIILGGWHP
                     SLLPVQTLAAPYIDYIVRGQGEDTLLELVQHIEDGASVDGIQGVGYKEDGKLRMNPER
                     PLRPLIEMPPKAYHIADFDAYERLCGRRWAMYTSSLACPFNCAYCTNGGVYGRKWNAL
                     PPDQFVEETMDLTRRYRLEMLWVVDDNFLVDLDRARGIAEGLVRAGADYTWSIQATTN
                     LTARLSIEDLKLLRRAGLRQICQGVDSGSPKILKLMNKEFQNFDSIYESAARCLEADI
                     RPSFNIIFGFPGEGPKERRETIQFIMDVCRRFPGSEFWTNIFTPYPGSPIMDRALELG
                     IKAPDSLEEWADYFPRYTVLPWLKGKQHERLQRMRDYLRIAFDRIPIAADTRKPWVRK
                     TQKALSLPARWRLDHDVYGFPAELWINKKLKQVSQAAKPAVDAKRLEPNPANA"
     gene            13576..13923
                     /locus_tag="VFA02_00570"
     CDS             13576..13923
                     /locus_tag="VFA02_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZU08367.1"
                     /translation="MLKKSSLLLLLVVISVFAHALDTTRTIEVHAKRYAFAPAEITVR
                     KGETVKLELISDDVPHSLLIKELGINQTITKDHPAEVTFTPASVGDFHGKCGRFCGSG
                     HGKMDFTVHVTQN"
     gene            13923..14912
                     /locus_tag="VFA02_00575"
     CDS             13923..14912
                     /locus_tag="VFA02_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08368.1"
                     /translation="MTSECVEVSGHGPLWLGAQFPLRARYFPLGFPLELATNSALVMA
                     AAGESWGHFSRKFDAPPLELSITVDATDKTPCALPHTPISRLQMDHLFHIVDAHNFMV
                     SDLENGRSFGWITESTASATSYLRYHFLEAAGLSMISALRAVAIHAACISLHGNGVLL
                     CGDSGAGKSSLAYALALRGWTLTSDDASYLPLGRRDRLIVGNPSKIRLRSSSPKLFPE
                     LSGRPVCSRFGGKPTIEVFLNEVPGISVSDTAFIRHVIFLDRTRPSVHELRPLDKNTA
                     FSWFRHSFLTGISSSAAQESALDHFLDVNVMRMSYQDLGWAVERLRQLVLTGA"
     gene            14916..16202
                     /locus_tag="VFA02_00580"
     CDS             14916..16202
                     /locus_tag="VFA02_00580"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZU08369.1"
                     /translation="MFLPAKPSSRPLRVPREIAALLRALQLGRPDASQLHALTDTEWK
                     SLLEFSDLAHLTLSLAQLDQQGFPDWVRHRLEKNLADNAARFDLVVNTYQEAAAALEL
                     AGVDYLVLKGFTQSPEYVPGPRLRMQSDLDLYCPQEEVPEARAALEALGYVSDDQMDY
                     SHADHVPTLYRKGRWTWRGNMFDPEMPLSIELHFCLWNEAELLFPAPQIDTFWNRKKT
                     RTLENISFPALDPIDQLGYTSLHILRDLLTADWMIHHVYELAFFLHHHAHDHAFWTRW
                     KELHPERLRQLQAVAFFLARDWFGCNLSAENKTQIHRLPAEILQWLERFSGSPIEGMF
                     KQNKTMLWMHTALLGTSGRKWPLVRHKLLPVIKPRINSPSLKWSYRRPRKIRHLPPRL
                     RYLVHFASRIFTHTRLVPATLFQGLVWKFHASRLPE"
     gene            16344..17966
                     /locus_tag="VFA02_00585"
     CDS             16344..17966
                     /locus_tag="VFA02_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU08370.1"
                     /translation="MAVPRHEINLPYKERLMLKHMFLACALAGSVAATSYAAQDSDDF
                     GYKTRDVKHVLLISVDGMHAIDLTNYISTHPDSTLAELTHHGIVYDNNATSTPSDSFP
                     GLAALLTGGSPTTTGFWYDVTWNRRLSPPAQTTPYGIPGSATACPNVQGTQVGFDEEI
                     DRDYTKLNGGGGINPKWLPRDPAHGCAPVYPHEYLRVNTIFEVAKSAGLYTAWSDKHP
                     SYEWVNGPSGRGVDDFYAPEINSIPVALPGIPGCDPLPDPANATPSNAWTDSIQNIQC
                     YDTLKVNAILNEIAGRTHDGKSSAPVPAIFGMNFQSVSVGQKLKGNGYYDVTGTPSVG
                     LESEFDFVDQSLGKMISELKQRGLWDSTVIIIGAKHGQSPIDVQKVTKIPGSTPQNII
                     GSAYAFDISDDGSLIWLKDPSLIADVLSNLSTPAAQEQLGVQEIFAGPSLRTRWNNPE
                     TDDRTPDIILKTNTGVIFTGGSKIAEHGGLNEDDYHTALLVSGAKIRHRVVKSPTTNQ
                     QVAPTILELLGLNPDKLQAVQKEGTPVLPFVR"
     gene            complement(18063..19952)
                     /locus_tag="VFA02_00590"
     CDS             complement(18063..19952)
                     /locus_tag="VFA02_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZU08371.1"
                     /translation="MSSCCTPATIEIETRPRVAGKIHTVALIGPPNSGKSTLFNRLTG
                     LRQKVANYPGVTVEQHYGKMSGIDRGDLILIDLPGIYSLNTHSEDARVAVDVLHGRMP
                     GAPAPDAVLLVLDSLHLQRQLMLAAPILSLGLPTMVLLNMSDLMETRGGEVDTLALAR
                     ELGVPVAKISAARGTGLDAIKHFLDQKSEPVKAAAPKLELPVMGNPRSYRQWATGIST
                     RTKYKAPLSSEWTRKLDSVLLGRITGPLFFIAVVFAVFQVVFSIGRPLSDALQDLLNA
                     SGDKVGLLLGHGWLQSLLIDGVWKGVASVLVFLPQILLLFLFIGILEDSGYLARAALI
                     ADRMMRAIGLNGKAFIPLLSAYACAVPAIMATRTIENKRDRFATILVTPFMTCSARLP
                     VYLLIIAAFVPNKPLLGNFFGLQAAVMVGLYVLGFLAALFTARLLKSSILKASSAPFI
                     LELPQYRVPTLRSLGLRLMDRGMVFVKQAGTVILSVMLVLWILSHIPFHADLPQSLIG
                     RVGQFIEPVIRPLGFNWKIGIGLLSSVIAREVIVGTLGNLYGADPATQSLTLQAALRH
                     DLTLGGAMALVVFFAFAMQCTSTLAVVKRETNSWKWPALQFAYMSVLAYLAALATNVL
                     VSKFF"
     gene            complement(19955..20191)
                     /locus_tag="VFA02_00595"
     CDS             complement(19955..20191)
                     /locus_tag="VFA02_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZU08372.1"
                     /translation="MAALSELRVGGTGVIEVLRLPDEIQHHLMHMGFIPEARVRAVRR
                     APAGDPTVYAVDGMEVALRRDTARWITVRELEEE"
     gene            complement(20418..21470)
                     /locus_tag="VFA02_00600"
     CDS             complement(20418..21470)
                     /locus_tag="VFA02_00600"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZU08373.1"
                     /translation="MFYPTNNLRIKATKVVLPPVFLQEEIPVTDQASATVFQARNEIS
                     AILNGKDHRLVVVVGPCSIHDPKAAREYAGLLKGAIAEFSKDLMIVMRLYFEKPRTTL
                     GWKGLINDPHLDESFRINDGLRIARHLLVDLAEMGVPAGTEFLDMISPQYVAELVSWG
                     AIGARTTESQVHRQLASGLSCPVGFKNGTSGNVQIAIEAIVSAGHPHTFLGTSETGQS
                     AILFTEGNPDCHIILRGGRQTTNYDAASVASTAEQMEKAGVQPRIMIDCSHANSGKDH
                     RKQGAVCRNVAEQIAGGNRRIIGVMLESNLVAGAQQLVSGKPLTYGQSITDACIGWEE
                     TYELLGNLAEAVRKRG"
     gene            complement(21554..22225)
                     /locus_tag="VFA02_00605"
     CDS             complement(21554..22225)
                     /locus_tag="VFA02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZU08374.1"
                     /translation="MRQPYFITHLVKDLRLEWRSKDAINSMLFFALLVVVLFSLAFDP
                     TRASSRQIAGGILCVAAMFASVSALNQAWARELRHQVLDAQRMTPAPAAALFLGKAIA
                     NFLFVTVVELILTPVFIIFYNLHALGNAWLLALVLPLGTWALVVNGTFFAALSIRTRN
                     RELLLPLLLFPLFIPALLAMVLATNYILTGEQAPGLWIRMLLGYDVVYTTICLLLFEV
                     VLHAE"
     gene            complement(22222..22731)
                     /gene="ybaK"
                     /locus_tag="VFA02_00610"
     CDS             complement(22222..22731)
                     /gene="ybaK"
                     /locus_tag="VFA02_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HZU08375.1"
                     /translation="MSTNVRRKTSPATFATHYLDSLHISYELRDYELDNENFSAILVA
                     EKIGLPPEQVFKTLLCVTSEKEHIFAVVPGNAELDFKKLARAAGARKVEMVPLKDIQP
                     LTGYIRGGVTVFGAKKDFPVFVDETIGLFDVVSVSAGTRGMQVVLSPEDYIRAAKATV
                     VPLTKEAAA"
     gene            complement(22787..23305)
                     /locus_tag="VFA02_00615"
     CDS             complement(22787..23305)
                     /locus_tag="VFA02_00615"
                     /inference="COORDINATES: protein motif:HMM:NF021163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2393 family protein"
                     /protein_id="HZU08376.1"
                     /translation="MDLSGQAPQEQERRSILPWIIAGGVIVVAVVLLLVFSGPKPRPA
                     NPGGAGLAAPDPYAASLPITGLQMSESANFAGGKVTYVDGHIANKGDKTVTGITVQVA
                     FRDIGNQLVQKETLPLMLIRTREPYVDTQPVSANPLKPGDERDFRIILDSVTQDWNGQ
                     YPEVRVIQVTSK"
     gene            23397..23729
                     /locus_tag="VFA02_00620"
     CDS             23397..23729
                     /locus_tag="VFA02_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08377.1"
                     /translation="MPPKTVNEAPPEFAAIAAAFAKTPDITAGKLMSSYGLKVNGKIF
                     AMYGRGKFVAKLPRQRVDELVASGAGERFNPGHGRLMKEWIAMASHNGDWLSLAREAY
                     SFVKTGKM"
     gene            complement(24032..24307)
                     /locus_tag="VFA02_00625"
     CDS             complement(24032..24307)
                     /locus_tag="VFA02_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HZU08378.1"
                     /translation="MRISRDKLNKLAHVVADTLAEIDEVEFLEDRNTIRQEARKALEH
                     LLVEEMRIDQAARQKIESQRKIILEGSQEWEILYRKYYNDEVKKLGI"
     gene            complement(24304..24582)
                     /locus_tag="VFA02_00630"
     CDS             complement(24304..24582)
                     /locus_tag="VFA02_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HZU08379.1"
                     /translation="MIFSKEYVGYLARHTVRHLITAKMIKTDKLPLVEERVYNGLLEE
                     LQLEDRINEEVRVILEAYQDEMRRTGASYPEMFKKVKQELARKYKAVL"
     gene            complement(24806..25171)
                     /locus_tag="VFA02_00635"
     CDS             complement(24806..25171)
                     /locus_tag="VFA02_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZU08380.1"
                     /translation="MAKFQRHIFVCTNAREPGSARPSCSHSGSNELHTLFKDKIKDAG
                     LKSAVRANKAGCLDQCEHGPTVVVYPEAVWYGFVKPEDVDEIVSEHLVHGRPVKRLML
                     DEDCINTANCPHRMVLVKG"
     gene            25253..25726
                     /locus_tag="VFA02_00640"
     CDS             25253..25726
                     /locus_tag="VFA02_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08381.1"
                     /translation="MMQPDNLISLKDDMIAFIAGHGMRRLPGYIGEEVPSVTWADSGN
                     PDSWKDFVETAKAAGAPFITMSDFVLEKDDLELLLEELHDLDFPNEESSDTEEAQTLI
                     NYVGKTGYIQLGFVHQGVAFLHESSTAWYDRYQRLLESVEDFGDIVFEQNEDEDE"
BASE COUNT         6194 a         7392 c         6380 g         5782 t
ORIGIN      
        1 cacatcaact ctatatttca ctttatgaag agctgcgcgg cgattatgca ggctggcttt
       61 cacgcttatt gttatttttg ggagtcaatc cggcatttgt tcccgcgcat tcagaggtcc
      121 catctacgcc gcatcttctc aaactcagag ggttgcaaca gttcaggcat ctcggaaacc
      181 gcatcattcc aggccgtatc aggacacgta taaaaaagca tgtctaccag gaacgtctgc
      241 ctgagttgca tgccgaggac caggcaattt tagtgcgcta ttatcgcgac aatattctga
      301 aattgcagga ccttatccag aaagacctga cctcatggct gcattaaacc cgcaattatg
      361 tgggcttctt tctaagaaac aaatactcca tctcgctggc aatccgcttt ccacggctgt
      421 tccaatatgc cggaaccttc tcagctattt gaaaatcacg gggagcagca atgttagcaa
      481 cgcgattttc cattacacat aagaaagcat accgggtcat gcgggagaca ttgttcagat
      541 atacgcctgc tgctacttca ggcaaatcag acatcacgtg ggaacaaaac gtgatatctg
      601 cgcaatcagc aggcattttg tccagttcag taaccggcat cattatgatg tctgggcaga
      661 cgccctgctt gtcttctcca tagagaagaa gttgcatttc tggaaatgcc ttcgcgagat
      721 aataagtggc caatgcaagg ctttccggga gatcgaagtt gatgtaagtg atgtcctttc
      781 gtccgcggaa aagataatag gccattccac caaaaccgcc gccaatttct gcaacgacgg
      841 cgcgtccgcc cctcagcaga tgtgaaatct tccacgcaca ataatgatgg tacgcagatg
      901 aggtctgtac caggacatca tcaagccaaa tcccaaaagg attccctatc tccggcccag
      961 caagctcatg cagagaacat cggctttccg tttgccgcgc ccagtactcg agccggaaca
     1021 actcatctgc gagataggcc ctccaatgga tactcgatgc agctctgcaa aacttctcaa
     1081 aaggcaatcc gcatggacgg ccaaccaatc ctgcagagca gttgtcccga aagaagtttc
     1141 cgtacatgcc ccgcaaagca tctaagtttt gcgttgataa tgcctgcatg gcaggacgaa
     1201 gcgcggattg ccgcagattc tcccaccact gggttggtcc gtacacttcc agggctgtgt
     1261 gctggtgctc cattgcgctg gcatacgcgg cacagattct ttcgaaaagc gcgttgtctc
     1321 cactcgcatg actcgtgcgc ggtgcaaatc ccttccaaac agtttgccca ctgtaacgag
     1381 ggtcttttct aaagcgccac gctccgcggt atgccagtat tgccctgtct aaatatccag
     1441 caacaaggcg catcgccatc cttgccgcga gaattggatg acgcagatgc ttggaccgga
     1501 atttcatgcg gagctatggg ttaagaaagc gcagaagcgc ctgctcgtct ttttcgctca
     1561 aattagcgcg aacacgcatg tgccggacga ttgtgccaga gatacgcggg gagaatccct
     1621 ccggagcgtt gtgacagcgc gaacagtttt catcaaatac ccgctcgcct tcgctctttt
     1681 gtggttcatg tacggatttg ggtcttgctt catgccggtc agaacgcggc tgagcagcac
     1741 tcatattgct tgtctgcgcg aatgttgctg acacagcaag caatgagaca acaataagca
     1801 agtgtttgca gcttttcact atttgcctct ccatgcaggg aaaagaaagc gatagacgat
     1861 ggctgtttcc cagatattgc gattgcccgt tgatgagaat tgccgcgcat agctggtgtt
     1921 aatgcgcact tcatgcggca gatgatagtc cagtccaaag tctgcgcgct ttgtgtctac
     1981 cgatggcaag ccatcagtag catcgggact gttgcggaaa gtctgctgcc agcggaacac
     2041 cggctccagc ctgccaataa gactctcagc gccaccaaat tgtgacaagc gataatcacc
     2101 ttcgatccaa agcccctgcg agtgttcacc atgcgcgtat tctgaccgga ccctcagcgg
     2161 aacctgtgct ggttcccacc acacgtgcgc tccataaaaa ttttcctgtt tatgctcaag
     2221 cagatgtcca taggaagttc cgatttcaag tcgcgcatgc ggaaagtacg cattgactct
     2281 tccaccagag gcccgcgaag actgaaactg ctcattcgta ctgcccgctg agaagtatgc
     2341 agcatagtca atcgaaacct tcgatgtgga ccaggcattg ccacgcaaca ttcctcctgt
     2401 ggcgctgccg ctcgtcattg tgcccaggct cgaaatgagc ggagcatcct gaaaattgct
     2461 gatccagata ggtgagagcc ggtcgttata cgtcccaaac ggaataagaa aataaccgcc
     2521 gacaactgtc acatggggcg ttgcaatata gtcgccctga agataagaga gagcgatgaa
     2581 gtgctgactg tcataacctt tacctgtctt cggaaaaaca gtatccagca ggatcgctcg
     2641 tgactcaacc agcaagtgat cgcccagcgg cgcagcaagc aaaggcataa tcacaggtgt
     2701 atatgaggtg ttgcctccgt tggtgctggt ttcaaagccc atcccgcccg aaatcagcgg
     2761 agtattctgg gcatagaaca cagaggcaaa acccattacg aacagaaaag acatagcgct
     2821 gacaaaagaa cgcaacatgc gttcaatgta tggttctttc atcaggaaaa tgtcactaag
     2881 ttgtctcaga ttttgtcatc ggattgtcat ctatctcgtt atagtgcaga gagcaaacgc
     2941 agttgcggca cgtgggattt tcattgattt gcagagacgc ccgaaattca agtgcgcgct
     3001 tgctgttcag aatgcgctct aaaggagctt cgtccaggtt tccaatggct tgatgaaaaa
     3061 agcatggacg cacagtaccg tccgcttcca gaattgccga cacccatgga gcattgcatc
     3121 gtggtgcgac tggctcctgc tgtccaagtg ctgcgcgaaa atgctccaca atgcgtcgca
     3181 gtttttccgg gccttcgcgg acgaatctgc cggagtatcg cgcaattatt ttttcgacta
     3241 caactgttag tgtttcgatt tcttctgcgt tcagctgaac gctgcttttt cgtgtgtcgc
     3301 tccagccgtc cgggcgattg aacgctgagg aggtggtgtc cgctgcaaga aatgagatgg
     3361 aattcaggcc gagtaccatg gccgcctcta tcgtggcttc gagagcgtca tgatttctct
     3421 tctgcacagt gcaacgggca tgaatttcaa tttgcggatg cagacggcga acagcagaga
     3481 ttccttctgc tagccgttga tacgcatccg gcacgcggcg gatagtgtta tgtacggccg
     3541 gaggaccatc gagagaaaca atcacatcgt ctacagatga cccgaccatc tgtgcacagc
     3601 ttttaagcag aagtccagtt gaaagcaacg tggttcggat gttttcggca cgaaaaagcg
     3661 atagtaaccc gcctagttct tcatgcaaca aaggctctcc accggaaagt acgacccacc
     3721 gtacaccgag agcgcgaatg gattccatat gtggcgcaag atcagaacgc cagagttgcc
     3781 ttgtctcgcg gatgcgccag atgtcgcaca tcatgcagcg gcagttgcag cggttatgtg
     3841 ggaaaagaac aaggacagga agctgcatga tcctgtgctg ctgcaatttc tttccggaaa
     3901 ttgccggcgt gatctgcaca agggtgctca taccaacgcc tctgcaattt gctcgtgata
     3961 cgtttgtatt tcgcgaatct tctcacatgt gctgaccgga aaaagccggg tagttcctcc
     4021 ccacgttgat tcttcgaaac catccagaag gtgcgccgaa acacgttctg aaaacaccgg
     4081 agagacaaat tgaagcgcgc cgtcgcggat gacacactga atatcattgc gcgacgccca
     4141 aagcagagtc tccgccggac tctttcctga atctcgaaga acaatcaaat ctgcagcagc
     4201 acccgcgcga atagagcctt cgccattttc aagacgcaag gcagccgcgg cccgcgttgt
     4261 caccatggga tacagggctt ccgcggacaa tccactgtgt tcatgcgcga aactcaactc
     4321 gtccagaaga tcgcctacag cagagagtgg cgaatcgctg cccaggagga gataaggata
     4381 ggctctcact gtctttgcag aaaatgtttt gcccaacgta aagatgttcg aagaagggca
     4441 ccacaccagc cagactttcg ccgctgaaag ctcgtgcagt tgtttccgcc gaatgcctat
     4501 tccatgcacc agaacaagcc ggtcatcaag agggaccatc tgcttcaaac ggtctacttc
     4561 tttcgctgct gctgcgtcgg ttccttctgc aagatgcaaa aagaaaggcc actcactcgg
     4621 agtctgatga taagcagctt ttgctgtctg ttcatctttc aaagaatgcg cccagccata
     4681 gcgtgacagc acctttacag gaaagtcgtg tgtaaatatc tccgcatggt agggattgtg
     4741 ctctgcaact gtagtggccc cgcagagcag attgcggagc cctcctgacc acaggcgaag
     4801 gtgtttagga acagcgagga gatcgcgtac aggcggccca tcaggccggt aaatgtcctg
     4861 cgcccaagct tcccagttcg gataaggccc tgaccccagg cgcggaaaca tcccgaactc
     4921 aaggtggtcg tgcgcattga tcagtccggg caaaacgatg tagccatcaa ggcggatgat
     4981 gttggctgtt tgtgttgcat gagaaatccg gctgtcgcag acagataaat cagtatgaac
     5041 tgcttcctgc gcatctatcg caatgcgtcc gcctgcaagc aaaagacatg gatgcctgga
     5101 tttcatttct ttgcaaaccc tgctacgaaa atggcgggct gctgtgatgt gatatcgaac
     5161 atatcttcgc atccggcccg ctcaaaaatg gcacgttctg gtctgccaaa gcacggctct
     5221 ttgcaaaact cttgcaccag ccctgcccca tacagtccct cgcgcaagct gtcgagagaa
     5281 tatggatagg tatgaatcga gtacgaactg ccctcgacgt tgaacccact gcgccagcca
     5341 tgtgcacgcg cccgcggatg aagatcagaa atcagcaatc ttcctcccgt gcgcagaaga
     5401 cgcgcagcct gtgcaacgaa ggcatcgagg gagggcacat atgaaagcag aaaagaagca
     5461 gccagcacgt cggcagacgc gcttttcaat aatggtgaaa gaacatctgc gcagatcagt
     5521 tccacttgag gcgggcactg ccgctgggcg acagagagca tcgctggcga tatgtcaact
     5581 cccaacagag atgcagctcc acactctgct gcatatctga gccagcggcc cgtaccgcat
     5641 cccagatcca tcacatgctt gcctcgaagt tcttcaatct tcggaagcag tacgcgctct
     5701 tccaaggcca gcacaggatt atcagcggca tcatagcaag gtgcccataa ctggtaagcc
     5761 tccacagcag aaagggtgat catatgcttt ccacctttgg cttgcgaagt tggatccagc
     5821 gatgcgccag ttgcaactca acaggaaaag cgtagatgcg aaatctgtag cgccatgcgc
     5881 tgagcgcctt cagcaatacg cgaccccatc tgggagcacg gatgtcctgc actgtgggcc
     5941 agcggcacga cacaacgact tcaaagttgt caatcagctt gcgaatgcga cgcctcatcc
     6001 acggcgttgc agggtctttc ctgaccgtga agttgtacca acgcggagtt gcccactcct
     6061 caggcgtagt aggaaattcg attttgccat caatatctcc gtacattttt tcgcgctgag
     6121 gaacaggcgt atagtgctga atgatgattt cagaggcagg attcagccgc ttgatcctgc
     6181 gcacaaaatt cagcgtctcg tttgtgtcgc gctctggatc attcgggttc ccgactacaa
     6241 aggaaaattc tggaatgatg ttaaagtcgc ggatacgccg cgccacggtc agtgtctctt
     6301 ccgtcgtgat gcctttgttc atttccttca gggcccagtc agacccggat tctgcgccga
     6361 agaagatcat ggtgcatccg gccttgcgaa tcagtgcgag cgtactgtca gaatagcggc
     6421 tgacgatatc tacgcggccc tcgcaccacc attgcagacc cagtggagcc agcctctcca
     6481 tcagctcgcg cgtatggtct tcacgcagaa aaaaattcat gtcataaaac tgcaccccat
     6541 caatgcggta ctcctttaca agatggcgca gaatcgcctc agtacgcgcc ggcgattcca
     6601 ttttctgacg gtttccaaat gctgggccaa ttccacagaa gcggcattga aaaggacatc
     6661 ccacgctggc ctggtgtgcg gcggtgcgct tgccaaagta ggacgggcga agatacttct
     6721 ctacaggaat gctgttgaac ggactccagg gaaagacatc cggtccaagc atcatccgct
     6781 caggattgta atggtgcctt ccgtccggag ttttataact gagtcccgca actgatgaga
     6841 catggcgttg cccgcggact acttctatca gctcaagcaa agtcacctcg ccctgtccac
     6901 gcgcgatgta gtccacataa ggagcattca gcgccgcgtc cggatagatg gatgggaaat
     6961 aacctcccca cacaatcggc acctgcggga accatgtgcg aatgtttttc gatgtgttca
     7021 tcgccgccac catctgcggg ccgggcatga cgcttacgcc cagtagttcc acatgatggt
     7081 gctgtacaag gtcagcgatg gtcttatcgg gatcgggatc gaggtttccg tccacaatgc
     7141 ggtattcctc ccgcccttcc agtactgcgg cgagcgccag cacagaaagc ggaaagcggc
     7201 ggtttgcagg ccgggtagcg cgaggataat aaagcacaat catgacttca ctacatatcc
     7261 gatatagaga aagaactgtg atggctcacg acggcccttc cagtgcgcga tccacggacg
     7321 catcgcccag gagagcccgt accagggttg aattttccgc cagagaatgc ccaggcgtga
     7381 aagatcgtcc ataatttccg gggtaagaaa ttcctggctt gccatgcggt ctgaagaaaa
     7441 tccatacttc tcgcaaaacg cctcatggcg ctcccttttc atggccatgc cgctttccag
     7501 cttgctgtat gtgggcgaat ccataatgac aatcgcagcg ccatgacgca ggcagcgctt
     7561 tgtttctatt agtgttttgc tgtagtccgc agaataatga aacgaagcat tgaaaatggc
     7621 gagatcaaac tgctcatctg caaatggcag acagtccatc gcagcctgaa accgcggaaa
     7681 catacagggc agaacttcgc tgtaatgatg tgctgcttcc agtccatcaa aggcgcttgc
     7741 tgaaagatcc acggccacag gcgtatggcc ttgcaaagca atcctgtagc ttagccagcc
     7801 atttcctgct ccaagatcaa gcacacgcaa tgcgtcgctt ttcgcaagca ccttctgttc
     7861 cagtacgcga tagctctttg ccctgatctt ccactgccag gagaagttcc ctgtgaggtc
     7921 ctggaatgga agtgcgcgat aaaactcact gttctctgaa cctctgcctt cctgcaagcg
     7981 aatgctctcg taatctcgca aaaactctgc atactctctt tcctgttcga aagtgagtgc
     8041 acgccagacg ccattctttt gttcgagcac aaacccgcaa tgttcacaga ggagtactga
     8101 tggcccctgc gatgaaacaa acaagtcaat tgacccagac ggcctcctgc attgtgggca
     8161 cagcagcgca agcacgtcat ccatagcagc tttgggtgga acagacgcct ttgcaaccat
     8221 cgtgctcatg atgcgctcct cacaaattcc agcaggatgt ggtcacccat ggcccttgcg
     8281 cccggcattc gccgcaaatt ctcatccaaa catgccagca aagagagaag gcgcggacgt
     8341 ccatgaaaaa aactttctgc ataagaaggc ggaacagcca ctccgattcc ttgccagccg
     8401 atgagagaga actccggcga aaagattttt ttcagccttc ttactgatgg ataacagacc
     8461 tgaatctcct gcccgccaac acgcgctgac gctcccgatc cgcaaaatct gcgaaaggct
     8521 ttttttgcat ctcccttaga aagaaaccag gccacttccc acaaacagaa cctcccaaac
     8581 aagcacaaca gaagcttgcc gcccggacgc accttccccg ccagtccagc tactacagct
     8641 tttaaatcgc ggctacaatt caggctggca aaattcgaga acacgccatc gaattcgccg
     8701 tcaacagcaa tcttgtgcag gtcctcgttt gcgcataccg caaaactgat gtgcgtacta
     8761 tggcccattt cccaggcacg ctgtttcgca attgcaatca tctgcggaga ggcatcgcac
     8821 gccacaaccg acgctcccag acgcggcaaa gccacggcat cagagcctgt gccacatccg
     8881 agttccagaa ttctatctcc cggatgaaat atctcccgcg caacactcca tacctgattg
     8941 cgttgcagaa tgccaatggt tgactgcgta aagatgcggt cataagcagg cgcgacagca
     9001 tcaaaggccg cagccgtcat gctgcacctc cggcatagag catcttccgc tcttgttgaa
     9061 cggcgcggac aagctcgtct ctgcgcaaca gcgtcatctt gtcctgcaca gaagagtttg
     9121 tgattcggct gagttctact tcatgccgca gcaactggtc agcaacttta taaaattcac
     9181 gcgagcggcg gccggtaatt tccagttcac ggtctgatgt ctgtgcccag ggcttgatct
     9241 gcaccagccg cgaagcgaca tttttgtaat aaggtgtacc tttaatcgga tatgaaaccg
     9301 tcgttagaaa gatgtccgga ttcgaagagc gaacatgatc aatcgttgcc tcaatgtcct
     9361 caagctcctc gccctcatat ccccacatca ggaacattcc tgtctggatg ccgtattgcc
     9421 tgcacaacgc cacggcattg cgcacggctt cgattttcac gcctcgttgc atcgcatcca
     9481 gaatgcgctg cgaaccgctc tcggaaccaa tccatatgcg gaaacagcgt agctcagcca
     9541 atgccgcaat gaccttctca ttcagccggt ctgcgcgcga gatgcactca aacggcaggc
     9601 ttagattgcg ctcacgcaac tcttttgccc acgcgtccag ccatccgtga tggatcgtaa
     9661 aaacatcatc tgcaatccac agcatgtctg gttgataacg ctcgcgcagc catgccaatt
     9721 cgtccgcaac cagatgcggc tttctcctcc ggtgcgtgtg cccaaagacc tgatggctgc
     9781 accactggca ttgatagggg cacccgcgcg ccgttatgag cgagatagac cccatgccgt
     9841 gatgtttacg ccatgtctct acatactggg ctgtgttgat ggcctctctg gcaggccagg
     9901 gctgggcgtc aatgtccgca atctgcgcgc gcggagcagt ccggaaaact gcgccagaag
     9961 agtcgcggaa ggcaatgcca tcaattttgc tcagatcagg agaagaagat tgccgataag
    10021 catgcagcaa ttcttcaacc gtcaactcac cttctccgat gacaacaatg tctgcgcctg
    10081 cttccagata ttcgtccgta tatgcgcccg gttccggacc gccgaccaca gttttccatc
    10141 cggcctgctt tgcttcctgc aagatttgca ccacaacgga ccgcgtcatc aaattggcgt
    10201 aaattccaag aatggatggc tgctctgtgc gcaatcgttc tatgagttcg gccttcgtag
    10261 aaaacgtgct gtcaaaaact tcaaccgcat gtccctttga cctgagatgt gaacagagat
    10321 aaagaattcc cagcggcgga taaggcttca taatctgaag ctctttgggg tcttccttca
    10381 ggaagtagcc atgcgtcagc agcaactcag gtgccttcat gctctgcctc cgcacaacac
    10441 cggaagcagg gtgggggccg cgtttctgca tgtgatttca agattgaata cacggctcca
    10501 gagtgatgca atatctgcct ttgcggaagg caactctctc cacgaatcca cttcagcatg
    10561 caaagcatca cgtagggctt tataaaattc gttggaatat gccgcctgaa acaggacgct
    10621 caggtctccg ctgtccaccc agtttctctt gctgcccaac tgctgcttca cgcgatcata
    10681 aaagactgtg ttcggcaagg gataagaaac agacacgcct atgtcatacg gcctggcacg
    10741 gcgcacaagc tcgatcgtct gttcaatttc tttccagcct tcgcctggat acccgaattg
    10801 caggaagaag catgcgcgaa tgccggcatc gcgcaagcgg tgcgcagcct gcacaatatt
    10861 ctcaacggtc tgccccttgt ccattgcatt cagcacagac tgggcgccag actcggcccc
    10921 catccacact tcttcgcacc ctgagcggcg caatgcgtgg actgtttccg gggtcatcaa
    10981 actggcgcgg ctttgcatct tataaggaat ggcgcattcc cgcgcctcta tcaacgcagc
    11041 gaactcctgg atccagtggc ggtcgagagc aaagatgtca tctgcaaacc agagatgctc
    11101 aatgccatac tctgtccgca acaattcaat ttcttctacg acactctttg cagatcgcag
    11161 tgcaaacgca tttccaaata tcggcttagc gcaccagttg cattgaaaag ggcatcctct
    11221 gcttgtaagg atgtttgtgg accgcctgtc gtgcgccgtc ttccagatag aagcgtaacg
    11281 agacattgca acaagatcgc gtgccggaag cggaagatga tcaattgtct tcattggcgc
    11341 gcgaggcggc gttcgcacga tctcgccggt gggattgcga tatgcaagcc ctgggaccgt
    11401 ccattcggaa acctgtccat tatgtagaac agcattgagc agttccatca gcgtttgctc
    11461 gccttcgcct atcaggactg catcaaagcc ctgctgtaga taaacaccag cattgtccga
    11521 cgcgtctgag ccgtgaataa aaacgggtat gccgcgtttg ctggcttccg cctgcatctg
    11581 ccacgctact tcgcgcatcc ggcccaggca cattttcgac aggtagttga aactgtcctc
    11641 gtagatgacg acgacacgag gccgatactc ttcgagcgcg actgcgaatc cacgctccgg
    11701 atcatcgagc atcgaatcga acagccctac agagaagccg tgctggcgcg ctaccgcagc
    11761 agcatacatc gtccccagcg ggggataagg ctgttgccgc tcgagttgtt tgcggtcgaa
    11821 tgccagatga taggcgtgcg tacaaaggac atccacagcg gcggaatgtt tcattgcgct
    11881 catgcgtttg ccggattcgg ctccaggcgc tttgcatcta ctgcgggctt ggctgcctga
    11941 gacacctgct tcagtttttt gttgatccac aactctgcgg gaaaaccgta gacatcatgg
    12001 tccagccgcc agcgtgccgg aaggctgagt gctttctgcg ttttgcggac ccagggtttg
    12061 cgcgtatctg ccgcaatggg gatgcggtca aaggcaatgc gcaggtaatc gcgcatccgc
    12121 tgtaaccgtt cgtgttgctt gcctttgagc cacggaagca cggtgtaacg cggaaaataa
    12181 tcggcccatt cttccaatga atccggcgct ttgatgccta gttcaagggc gcggtccata
    12241 ataggcgaac ccggataagg agtaaaaatg ttcgtccaga attcgctccc gggaaagcgg
    12301 cggcagacgt ccatgatgaa ctggatcgtc tcccggcgct ccttcggccc ttcacctgga
    12361 aatccaaaaa taatattgaa tgaaggccgg atatcagcct ctaaacacct ggccgcactt
    12421 tcgtagatcg aatcgaagtt ctgaaactct ttgttcatca gcttcagaat ttttggagaa
    12481 ccagaatcta cgccctgaca aatctgccgc agtcccgccc tgcgcagcag cttcaaatct
    12541 tcaatgctga gccgcgcggt aaggttcgtc gtggcctgta tactccaggt atagtccgct
    12601 ccggcgcgta caagtccttc ggcaattcct cttgcccgat ccagatcaac cagaaagttg
    12661 tcgtccacga cccacagcat ctccagacgg taccgccgcg tgaggtccat tgtttcctca
    12721 acaaactggt ccggaggaag ggcgttccac ttgcgtccat acacgccgcc gttcgtgcaa
    12781 taagcacagt tgaatgggca ggcaaggctt gatgtgtaca tggcccaacg ccgcccgcac
    12841 aagcgttcgt atgcgtcaaa gtccgcaatg tgatacgcct ttggtggcat ctcaatgagc
    12901 ggacgaagcg gacgctcagg attcatcctc agctttccat cctccttata tcccacgcct
    12961 tgaatgccat ccacggaagc gccatcttcg atgtgttgca caagctccag cagagtatct
    13021 tcaccctgtc cgcgcacgat gtagtcaata tagggagcag ccagcgtctg cacagggagc
    13081 agcgaagggt gccaaccgcc gagaatgata ggaaatttcg ggtcccacgc cttgatagcg
    13141 cgcgcgatct cgaccgtctc gcgaatcatc ggcccggtta ccagagaaat acccagacac
    13201 agcgcgtctt ttacctcttc cagcactctg cgcttaaagt tgggagttat cgtcgaatca
    13261 atcaggcata tttcgtatcc tgcccgcagc agcggtgtag agaccgccaa caggcccagc
    13321 ggggccgtcg cttcgctgct ggaaaacgac gggaaaaaaa atacgatctt gcgccgggga
    13381 cgctttgcca taatcgtatt tccgttatgg catggcgaaa tacctgctcg tgtcatgctg
    13441 ttgtcactgc tttgtcactt tgttgtccat catcgaatga cagactcatg acaaattcat
    13501 gacgtggcgg tgacagtcag cgtccgctgc tttcctatac tcacctcatt gaaataaaac
    13561 catcaggcag gaaacatgct gaaaaaatca tcgctacttc tcttactggt tgttatcagc
    13621 gtatttgcac acgcactgga cacaacacgg accattgagg tccatgccaa acgttatgcg
    13681 ttcgcgcctg cagaaatcac agtcaggaag ggcgaaacag taaaactgga attgatttca
    13741 gatgatgtcc cgcacagtct gctcataaaa gagctcggaa tcaatcagac catcaccaaa
    13801 gaccacccag cagaagtcac gtttacaccg gcaagcgttg gcgacttcca cggaaaatgc
    13861 gggcgcttct gcggaagcgg ccacggcaag atggacttca ctgtccatgt gacacaaaac
    13921 tgatgaccag cgagtgtgta gaggtttccg gccacggccc gctttggctt ggcgcgcaat
    13981 ttccactgcg cgcccggtat tttcctttag gctttcccct ggaattggcc accaactctg
    14041 ccctggtcat ggccgctgcc ggagaaagct gggggcactt cagcagaaaa ttcgatgccc
    14101 ctcccctcga gctttccatt actgtggacg ccacagacaa gaccccatgc gcgcttccgc
    14161 atactcccat cagcagactt cagatggacc atctctttca tatcgtggac gcgcacaact
    14221 ttatggtgag tgacctcgaa aacggccgct ctttcggctg gatcacagag tccacagctt
    14281 cggcaacatc gtatcttcgc tatcactttc tggaggccgc aggcttgtct atgataagcg
    14341 cactgcgagc cgtggccatc catgccgcct gcatcagttt gcatggcaac ggcgtgctgc
    14401 tctgcggaga ttcaggcgca ggcaaatcat ctcttgcgta tgccttggcc cttcgcggct
    14461 ggacattgac ctccgacgac gcaagctatc ttcctctcgg ccgccgtgac cggctaatcg
    14521 ttggcaatcc cagtaaaata cgtctgcgta gttcgagccc aaagcttttc cctgaattat
    14581 cagggcgtcc ggtctgctcc cgtttcggag gcaagcccac aatcgaagtg ttcctgaatg
    14641 aggtccccgg gatttctgtg tcggacactg cgtttatcag acacgtgatt tttcttgacc
    14701 gtacgcgccc ttctgttcat gaactcaggc cgctcgacaa gaacacggca ttttcatggt
    14761 tcaggcattc atttcttact ggaataagct cttctgctgc acaggaaagt gcgctggacc
    14821 actttctgga tgtaaatgtg atgcggatgt cttatcagga cctgggttgg gccgttgaac
    14881 gtctccgcca attagtgctg acaggggcgt gacctatgtt tttgccggca aaaccctcgt
    14941 ccagaccact gcgtgtccct cgagaaattg ctgcgcttct gcgcgccttg caattgggcc
    15001 ggccagacgc ctcacaatta catgcgctca cggacacaga atggaagtcc ctgctcgaat
    15061 tcagtgacct ggcgcatttg actctatcgc tggcgcagct cgatcagcaa ggctttccgg
    15121 attgggtacg ccatcggctg gaaaagaacc ttgccgacaa tgcagccaga tttgatttgg
    15181 ttgtgaatac gtatcaggag gccgcggcag cattggagtt ggctggcgtg gactatctgg
    15241 tgcttaaggg gtttacccag tctccggagt atgtccccgg tccgcgtctg cgtatgcagt
    15301 ctgacctgga tctgtattgc cctcaggagg aggtcccgga agcacgtgct gcactggaag
    15361 cccttggcta cgtttccgat gatcagatgg attacagcca tgccgatcat gtgccaacgc
    15421 tgtatagaaa gggccgctgg acctggcgcg gaaatatgtt cgatcctgaa atgccgcttt
    15481 ctattgagct gcacttctgt ctttggaatg aagcagagct gctctttccg gcccctcaaa
    15541 tagatacatt ctggaaccgc aaaaaaacca ggactttgga aaacatttca ttccctgctc
    15601 tggacccaat agaccagctt ggttacacat cgcttcacat tctgcgagat ctgctgacag
    15661 cagattggat gattcatcac gtctacgagc tggcattctt tctgcatcat catgcacatg
    15721 accatgcctt ctggactcgt tggaaggaac ttcacccgga acggctccgt cagttgcagg
    15781 ccgtggcctt ctttcttgcg cgcgattggt tcgggtgtaa tctttccgcg gaaaacaaaa
    15841 cacagatcca tcgtcttcca gcagaaatcc tgcaatggct ggagcgcttt tccgggtccc
    15901 cgattgaagg catgtttaag cagaacaaaa ccatgctatg gatgcacact gcacttctag
    15961 gtacctcagg caggaaatgg cctctggtgc gccacaaact gctgcctgtc atcaaaccgc
    16021 gcataaattc accctctttg aaatggagtt accgcaggcc gcgaaaaatt cgccatcttc
    16081 ctccccgtct gcgctacctt gtccattttg cttcgcgcat cttcacccat acccgtctcg
    16141 ttcctgccac cctctttcag ggacttgtat ggaaatttca cgccagcaga ttgcccgagt
    16201 aggtaaatct cccgctgcac tcaattcaga aaattcattt caaaataaaa actcgcaccg
    16261 caacttttcc ttataacgaa tttttaaact gccattcatt gtgtgtttac cttcagggcg
    16321 tctggtttca cttacgtttc gaaatggcgg ttccccgcca cgaaataaac cttccctata
    16381 aggaacgcct gatgctaaaa catatgttcc tcgcctgcgc tcttgccggc tctgttgccg
    16441 ctaccagcta tgccgcgcaa gacagcgatg acttcggata caaaacccgt gatgtgaagc
    16501 atgttctgct catcagcgtg gacggcatgc atgccattga cctcacaaac tatatttcta
    16561 cccatcccga ctctacgctc gccgaactca cccatcacgg catcgtctat gacaacaatg
    16621 caacgtctac tccatccgac tcgtttcctg gactcgctgc gctgctgacc ggaggctcgc
    16681 ctacgaccac tggcttctgg tatgacgtga cctggaaccg ccggctctct ccacccgcgc
    16741 aaactactcc ttacggaatc cccggatcag ctactgcctg ccccaacgtc cagggaacgc
    16801 aggtcggctt tgatgaagaa attgaccgcg attacaccaa gctcaacggc ggcggtggca
    16861 ttaatccaaa atggctgcct cgcgatccgg cccacggatg cgctcctgtt taccctcacg
    16921 aatatctgcg cgtcaacaca atctttgagg tggccaaatc cgccggactt tacaccgcct
    16981 ggtcagacaa gcacccgtcc tatgaatggg tgaacggccc cagtggtcgt ggcgtagatg
    17041 acttctatgc tccggaaatt aactcgattc cagtcgccct tcctggaata ccgggctgcg
    17101 atccgcttcc cgaccccgcc aatgccacac ccagcaacgc atggactgac agcattcaga
    17161 acatccagtg ctacgacacg ctcaaagtca atgccattct gaatgagatt gccggcagaa
    17221 cacacgacgg caagagcagc gcgccagttc ccgccatctt cggaatgaat ttccagtccg
    17281 tcagcgtcgg ccagaagttg aaaggcaatg gctactatga cgtgaccggg accccaagcg
    17341 taggacttga aagcgaattc gacttcgtag accagtctct cggcaaaatg atttctgaac
    17401 tgaagcagcg cggcctatgg gattcaaccg tcatcatcat cggggccaag cacggccagt
    17461 cacccattga tgttcagaag gtcaccaaaa tcccgggcag tacaccacag aacataattg
    17521 gttctgccta tgcttttgat atttccgacg atggctcgct catctggctc aaagatcctt
    17581 cgttaatcgc cgacgtgctc tcaaatctca gcaccccggc agcacaggaa cagcttggtg
    17641 ttcaggaaat cttcgcaggt ccatccttgc gcacgcgctg gaacaatccc gaaacagacg
    17701 accgcacgcc ggatatcatc ctgaaaacca atactggtgt catctttacc ggaggctcca
    17761 aaatcgccga acatggtggc ctgaatgaag acgactatca cactgcgctg cttgtctccg
    17821 gggcaaagat cagacaccgc gtcgtaaaga gtcccacgac aaaccagcag gttgctccca
    17881 caattctgga attgctgggt ctgaatccag acaaattaca ggccgtgcaa aaagaaggta
    17941 cgcccgtcct gcccttcgta cgataacaaa ttcgcctcaa gatagaaggg cgtcctgact
    18001 tcaggacgcc cttttgttcc taccgaaacg actggaggtc gcgtagtgga gcgacctgca
    18061 gttcagaaaa attttgacac cagtacattg gtagccaacg cagccagata cgccaaaacg
    18121 ctcatatacg cgaactgaag cgcaggccat ttccagctat ttgtttcgcg cttcacaacc
    18181 gcaagtgttg aagtgcattg catggcaaaa gcaaagaaga cgaccagcgc cattgcaccg
    18241 ccaagggtca gatcatgtcg caaagccgcc tgcagggtca gcgattgggt cgcaggatca
    18301 gccccatata aattgcccag tgtacccaca atcacctcgc gcgcaatgac tgagctgagc
    18361 agaccaatgc caatcttcca gttaaagccc aatggcctga tgactggttc gatgaactgc
    18421 cccacccgtc caatcaggct ctgcggcaag tctgcatgaa aaggaatatg gcttaatatc
    18481 caaagcacaa gcatcacgct caaaatcacc gtgcctgcct gttttacaaa caccatgccg
    18541 cgatccatta aacgcagccc aagcgagcgc agcgtaggca cacgatattg cggcagctcc
    18601 aaaataaacg gggccgatga cgctttcaga atcgaagatt tcagcagtcg cgccgtaaac
    18661 agtgcagcca ggaaccccag aacatacaac ccgaccatca ccgccgcctg caggccaaaa
    18721 aagtttccca gcagcggctt gttgggaaca aacgcagcaa taatcagcag atataccggc
    18781 aatcgcgcag agcacgtcat aaacggcgtc accagaatcg ttgcaaagcg gtcgcgctta
    18841 ttttcgatgg tacgcgtagc catgatcgcc ggaacggcac aggcatatgc ggaaagcaga
    18901 ggaataaatg ctttgccatt caggccgatc gcacgcatca tgcggtcagc aatcagggca
    18961 gcgcgtgcca gataacccga gtcttccagt atcccgataa aaagaaacag cagcagaatc
    19021 tgcggcagaa agacaagcac agacgccacg cctttccata cgccatcaat cagcagcgat
    19081 tgcaaccatc catggccaag cagcagtccg actttgtcgc cgcttgcatt cagcaaatcc
    19141 tgaagagcat cgctcagcgg acgcccaatc gaaaacacca cctgaaacac cgcaaagaca
    19201 acagcaatga agaagagcgg cccggtaatg cgtcccagca gcacagaatc gagcttgcgt
    19261 gtccactccg acgagagcgg cgctttatat ttcgttcgcg tgctgatgcc tgtggcccac
    19321 tgccggtacg agcgcggatt ccccatcacc ggcagttcca gctttggagc ggctgccttt
    19381 actggttcgc tcttctgatc gagaaaatgc ttgatggcat cgaggccggt gccccgcgcc
    19441 gcgcttattt tcgctaccgg aacgcccagt tcgcgcgcca gcgccagcgt gtccacttcg
    19501 ccgccgcgcg tttccatcag gtcactcatg ttgagcagca ccatggtcgg cagcccgagc
    19561 gaaagaatcg gagcagccaa catcagttgc ctctggaggt gcagcgaatc caggaccaac
    19621 aaaacggcat ccggagcagg cgctcccggc atccgaccgt gcagaacatc cactgcaaca
    19681 cgcgcgtcct ctgaatgggt gttcaggcta taaattccgg gaagatcaat caggataagg
    19741 tcgccccggt caataccgct catcttgccg tagtgctgct ctacggtaac tccgggataa
    19801 ttggcaacct tctggcgaag gccagtcagg cggttgaata gcgtagattt gcccgagttt
    19861 ggcggtccaa taagagctac ggtatgaatt ttgccagcga cacgcggtct ggtttctatc
    19921 tcaatcgtgg ctggcgtgca gcagctgctc atgctcactc ttcctcaagt tcccggacgg
    19981 taatccaccg cgccgtatcg cgtcgcagcg ccacttccat cccgtccacg gcatacaccg
    20041 tcgggtctcc cgccggagcg cgccgcactg ccctgacccg cgcttcgggt ataaatccca
    20101 tatgcatcag atggtgttga atctcgtctg gaaggcgcag cacttcaatc acgcccgtac
    20161 cgccgactcg taattcactt aacgcagcca aggtcttttg tctgatgagg gaaaaacccc
    20221 tttcactaca tcatacagca aaataggact aatttaatcc tagtttcgcc acacaagtac
    20281 ccaatcagca acattttccg ctagattccg cgtaaaacat ccaactgaga cagaaattcc
    20341 gctggattga ttgatgggtg ccccgcgcca agcgatgcaa aatcagaaga tcctgattga
    20401 cacagcagca cctatcctta acctctcttc cgcactgcct cagccaggtt tcccagcagt
    20461 tcgtatgtct cctcccatcc aatgcatgca tccgtaatgc tctgcccgta ggtgagcggc
    20521 tttccggaga cgagctgctg tgccccggca accagattgc tctccagcat cacgccgata
    20581 atacggcggt ttccacccgc aatctgttca gcgacattgc ggcaaactgc accctgtttg
    20641 cggtggtctt ttccactgtt tgcgtggctg caatcaatca tgatgcgagg ctgcacgcca
    20701 gccttctcca tctgctcagc agtagaggca accgaagccg catcgtagtt cgtcgtctgc
    20761 cttccgccgc gcagaatgat gtggcaatcc gggttccctt ccgtgaaaag aatggccgac
    20821 tgccccgttt ccgaggtccc cagaaacgta tgcggatggc ctgctgacac aatggcctca
    20881 atcgcaatct gtacgttgcc cgaggtgccg ttcttgaatc caactggaca cgacagcccc
    20941 gaagcaagct ggcgatgcac ctggctctcc gttgtgcgag caccaatggc gccccagctc
    21001 accagctccg ccacatactg tggcgaaatc atgtccagaa actctgttcc cgccggcaca
    21061 cccatttcgg caaggtccac cagcagatgc cgcgcaatcc gcaatccatc attaatccga
    21121 aatgactcat caaggtgtgg atcgttgatc agccccttcc accccaatgt cgtgcggggc
    21181 ttctcaaagt agagccgcat cacgatcatc aggtctttgg aaaattcggc aatagcgccc
    21241 ttcagcaggc ccgcgtattc ccgcgctgct ttgggatcgt ggatcgaaca gggtccgacg
    21301 actacgacca gccgatggtc tttaccgttc aggatggcag aaatctcatt gcgcgcctga
    21361 aataccgtcg ccgaagcctg atctgtgacc ggaatctcct cctgcaaaaa cacgggagga
    21421 aggacgacct ttgtcgcctt aatacgtaag ttgtttgtgg gataaaacat gggtctttgt
    21481 ttttaatgta tcagccggat gcgcctttaa gacctgtgcc ctgtccaggt gagggtcgaa
    21541 gaaacctcac tcctcactca gcgtgcagca ccacttcaaa caacagcagg cagatggtcg
    21601 tatagaccac gtcatatcca aggagcatcc tgatccacaa tccaggggcc tgttctcccg
    21661 taaggatgta attcgtcgcc agcaccatcg ccaacagggc gggaatgaac aacgggaaca
    21721 acaacagcgg cagaagaagc tcgcgattgc gtgtgcgaat cgagagagcg gcaaaaaatg
    21781 tgccattcac taccagcgcc catgtcccca gcggaagcac cagcgcgagc agccatgcgt
    21841 ttcccaaagc gtgcaggttg taaaagatga tgaataccgg cgtcagaatc aactccacga
    21901 cggtcacaaa taaaaaattt gcaatcgcct tgccaagaaa caacgccgca gccggagcag
    21961 gcgtcatccg ctgtgcatcc agcacctgat gccgcagttc ccgcgcccac gcctggttca
    22021 gcgcggaaac agaggcgaac atcgccgcta cgcacaaaat tcccccggca atctgtcgtg
    22081 aagatgcgcg cgtaggatca aacgcaaggc tgaagagcac tacaaccagc agcgcgaaaa
    22141 acagcatgga gttgatcgcg tcctttgacc gccactccag ccgcaggtcc tttaccagat
    22201 gcgtgatgaa atacggctgc ctcaagctgc ggcctctttc gtcagcggaa ccacagtggc
    22261 cttggccgcg cgaatgtagt cctccggcga cagaaccacc tgcattccgc gtgtccccgc
    22321 cgaaacggaa accacgtcaa aaagcccgat tgtctcgtcc acaaaaacgg gaaagtcctt
    22381 ttttgcgcca aagacggtca cgccgccgcg aatatatccg gtaaggggtt gaatgtcctt
    22441 gagcggaacc atctccacct tgcgcgcacc cgctgcgcgt gccagttttt tgaaatccag
    22501 ctcggcattt cccggaacga cagcaaagat gtgttctttt tcgctcgtca cacacagcaa
    22561 tgtcttaaag acctgttccg gaggcagccc gattttctcc gccaccagaa ttgcggaaaa
    22621 attctcgttg tccagctcgt aatcgcgcag ctcgtaggag atatgaagag aatcaagata
    22681 atgcgtcgca aaagttgccg gggaggtttt gcgcctgaca ttcgtagaca tgcttctgtt
    22741 gtacaccgga gtggggccgg ccaggcaatg aacgccccaa ggcacgctat ttgctcgtaa
    22801 cctgaatcac tcgaacttca ggatactgcc cattccagtc ctgggtcacc gaatcaagaa
    22861 tgatgcgaaa atcgcgttcg tctccaggct tcagcggatt tgcgctgacc ggctgcgtat
    22921 ccacgtaagg ctcgcgcgtg cgaatcagca tcaaaggcaa tgtctctttc tggacaagct
    22981 ggttgcctat gtcccggaag gccacttgga ccgtgattcc ggtcacggtt ttgtcgcctt
    23041 tattggcaat atgtccatcc acataggtca ccttgcctcc ggcaaaattc gcagattcgc
    23101 tcatctgcag acctgtaatt ggcagactcg ccgcataagg atcaggagct gccaatcccg
    23161 cgccgcctgg attcgcaggc ctgggcttcg ggccgctgaa cacgagcagt aggacaacgg
    23221 cgacaacaat gacccctcca gcaatgatcc acggcaaaat cgaacgcctt tcctgctcct
    23281 gaggtgcctg tccgctcaag tccaacgtgt tcctgcgatc atcagccatt aagcaggatt
    23341 ctacatcggc aggataaaat tttcgcgcgg cccaaaaccg cagaggatcc gaacctatgc
    23401 cgccaaaaac cgtaaacgaa gctcccccgg agtttgccgc catcgccgcg gcctttgcca
    23461 aaaccccgga catcactgcc ggcaaattga tgtcgtctta tggattgaag gtcaacggca
    23521 aaatttttgc tatgtatggc cgcggaaaat ttgtcgccaa gctccccagg cagagagtgg
    23581 acgaactggt cgcttccgga gcaggagagc gcttcaaccc cggccacggc cgattgatga
    23641 aagaatggat tgccatggcg tcacacaatg gcgattggtt atctctggcc agagaagcgt
    23701 acagcttcgt aaagaccggc aaaatgtagc ccgttttggg gatgcgtacc ccaaaaggta
    23761 cttcagcaga ttgattcaga acaaaattct gcaaaaaccg aatcggatgg gcgcaaactg
    23821 tgtctaaatg agcagaggcg gtgatgcatt ccgccgtgtc gtaaccgatg tagccaacga
    23881 ttttgtacga ggccgttatg gagcaggttc ccaatgcact gcattctggc gcacccgcca
    23941 cgcgcagaaa atcttccgag tatgtctatc aaatggcgac gctggcggcc gtcttgctgc
    24001 tcttgctgac ctccgttatc tgattcccgg ctcagattcc cagcttcttc acttcatcgt
    24061 tgtaatactt gcggtagagt atctcccatt cctggctgcc ttccaggata attttgcgct
    24121 gggactcgat cttctgacgc gctgcctggt caattctcat ctcttcgacc aaaagatgtt
    24181 caagcgcttt gcgcgcttcc tgccggatag tgttgcggtc ttccagaaat tcgacttcgt
    24241 caatttccgc cagagtatcg gcgaccacgt gggccagctt attcaacttg tcacgcgaga
    24301 ttctcacagc accgccttgt atttacgcgc cagctcctgc ttgaccttct tgaacatctc
    24361 aggatagctt gcacccgtgc gccgcatctc gtcctggtac gcttccagaa tgacgcggac
    24421 ctcctcatta atacggtctt ccagttgcag ttcctccagc agaccgttat aaacgcgctc
    24481 ttccaccagc ggaagcttgt ctgtcttgat catttttgcg gtgatcagat gcctgaccgt
    24541 gtggcgcgcg aggtagccaa catattcttt cgagaaaatc attgatccta atgggaatat
    24601 agcatgactg gccgtcaagg cggacgcgcc ttcgacgcag atttatcgtg tcatggagac
    24661 cggcacccag ggcttcgtca agcaatgggt cgggaccaca aatttcactc cagcagaaca
    24721 atgagcaatt tctgtcagca gcgtgcttct atccgccatg gtcttgcata gacgctatac
    24781 ccagcacctt ccacaaatca gcaaatcacc ccttcaccag caccatccgg tgcggacagt
    24841 tggcggtatt gatgcagtct tcatccagca tcagccgctt cacgggcctc ccgtggacaa
    24901 gatgttccga tacaatctcg tccacatctt caggctttac aaagccatac cagaccgctt
    24961 cgggataaac cactaccgta ggcccatgct cgcactggtc gaggcatccg gccttgtttg
    25021 cccgtacggc cgatttcagg ccagcatctt taatcttgtc tttgaaaaga gtatgtaatt
    25081 cgttactgcc gctgtgcgaa caggaaggcc gtgcgctgcc aggctcccgt gcatttgtgc
    25141 agacaaatat atgccgttga aatttagcca aatccaattc tcctttgaat atcgtacaat
    25201 tcggaaactt gttggttttc cactccgcag tttctgagag aataggcatt tgatgatgca
    25261 acccgacaat ctgatctcgc tcaaagacga catgatcgcc ttcattgctg gacatggaat
    25321 gcgccgtctc cccggctaca tcggtgaaga agtcccttcg gtcacctggg ccgacagcgg
    25381 caatcccgat agctggaagg actttgtgga gaccgccaag gccgccggag cgccttttat
    25441 taccatgagc gacttcgtgc tggaaaagga cgacctcgag cttctgctcg aagagcttca
    25501 cgacctcgat tttcccaatg aagagagctc agatacagaa gaagcgcaga cactcattaa
    25561 ttacgtaggc aagaccggat atatccagct cggctttgtt catcagggag tggccttcct
    25621 ccatgagtct tctacagcct ggtacgaccg ttatcagcgc ctgcttgaat cggtggaaga
    25681 ctttggcgac atcgtttttg aacagaacga ggatgaagac gagtagctgt gagcgctggc
    25741 gcagccat
//
LOCUS       DASRBV010000018        32723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_659850, whole genome shotgun sequence.
ACCESSION   DASRBV010000018 DASRBV010000000
VERSION     DASRBV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 32723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32723
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659850"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFA02_00645"
     CDS             complement(<1..131)
                     /locus_tag="VFA02_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZU08382.1"
                     /translation="MFENLSEKLQRAFKNLRGQGTVTEENIGEALREIRVALLEADV"
     gene            259..594
                     /locus_tag="VFA02_00650"
     CDS             259..594
                     /locus_tag="VFA02_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HZU08383.1"
                     /translation="MAGDLQLTCSDCGQEFTFTEADQAFFQERGYSTPKRCKSCRQAK
                     KSDSYGGGGQRSWSAPQGTPVICAGCGQQTTVPFEPRGDRPVFCKSCYQAQAGNRGRQ
                     QRDSGRRRY"
     gene            complement(598..1434)
                     /locus_tag="VFA02_00655"
     CDS             complement(598..1434)
                     /locus_tag="VFA02_00655"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HZU08384.1"
                     /translation="MTRKQALDLFLSSDLIGLGMEADAARRNAHPDDVVTYALGERIF
                     SGTDAGCCQAEAESSGIRIPLASQSSVEDLEETLRALRKKFPSLQILVCASEILLLAR
                     RSHFSLEDALFRLRSAGLDSIADNEALAMDDWLAVHRAAHRAQMKTTAHFIFGAGEGL
                     EQRLDLLDRVSDVQKETGGFIALAPRSHAPARELDAPTGVEYLKMMAICRLYLPEIPH
                     VQASSTAQGLKMLQMALRFGADDAGPVPSHATEEDVRRIIRDAGFRPAQRDLLYRMMV
                     LN"
     gene            1554..2759
                     /locus_tag="VFA02_00660"
     CDS             1554..2759
                     /locus_tag="VFA02_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU08385.1"
                     /translation="MEKYVIVGGGIAGLTAANALAGNGDRVTVLEQAHHLGGRAQTQQ
                     QSGYFMNLGPHALYCGGVAMRTFREWGIPFSGNFPPRGDRSYFVRENKLYPAVRDVTS
                     LMTTRLFSVREKLEAARIFSAFTNASAEEGETMRDWLERHARSERVRDFAATIARIAT
                     YTTDLDHLSAKAAVKQVQMAIRHNVWYLNGGWQTLVDGLRDRARSLGVEIRSEEHVTS
                     LRRLDADGIVLAVGPASVEKLTGVAVPDQRPLRMATLDIGMRRLPEGAAHVAFGLDQP
                     LYFSVHSESARVAPEGRALAHVAKYLGDSQPDPKALREELEDYASLVMPGWQREAEVV
                     RFLPNLTVSPMMVTPQGRPEVDFPGLENVTVAGDWVGTEGMLVDAAVASALKAARVIQ
                     GRKTRELIA"
     gene            2756..3637
                     /locus_tag="VFA02_00665"
     CDS             2756..3637
                     /locus_tag="VFA02_00665"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08386.1"
                     /translation="MTMDREAVFLELKPMLFSLAYRMLGTRADAEDVVQETYLRWREA
                     AEDEIRSPRAYLATVAARLSLDSLKSAQRKREVYMGTWLPEPLVGPLGQQSVDMAESL
                     SLAFLHLLESLSPPERVAFLLHEIFEMPYSELAAVLETSEANCRQLVTRAKKHIQQKK
                     RRFTVDREHHRNVLEKFLHACASGDPAQLANLLHQDVVLYSDGGGKVRAALNPIFGMD
                     KVMRFFAGIVKKNALRGYSAELAEVNGGPGAVFYKDGELAQVLDLEVDESGRIINIFL
                     VSNPEKLPHEVGPKLQR"
     gene            3669..4982
                     /locus_tag="VFA02_00670"
     CDS             3669..4982
                     /locus_tag="VFA02_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU08387.1"
                     /translation="MADQPPGTQLPGSGAVAFLSRDFRLYQLARVLVIIGAEAQTLAI
                     AWLVYQITNSALYLGYTGLTLFLPGLLFLLPAGHASDRFDRRQVIILCYTLQIFCTLA
                     LFLFAWHGLHRVWPIYAVLFLVGTGRAFSGPASSALIPHLVPKEHFVNAVTWGATVFQ
                     IATIAGPSLGGILYTLPLHGSIAGASMVFLFTLATLIVFVSLMLSLHVRPGRMEHRAA
                     SMEVLLAGLRYVWKTQLLLGSISLDMFAVLLGGATALMPIFAKEILHTGPRGLGLLRA
                     APAVGALTVSMWLTWKPIQRRSGAKMLICVGIFGTATVVFGLSKSVWLSLAALFFVGA
                     SDMVSVVIRSSILQLATPLEMRGRVSAVNSLFIGASNELGEFESGLTASWWGAVRAVI
                     YGGLGSLAITGLWSIFFPNLRRADRLTAEALLQVEEEETAQEVRR"
     gene            complement(5059..5382)
                     /locus_tag="VFA02_00675"
     CDS             complement(5059..5382)
                     /locus_tag="VFA02_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08388.1"
                     /translation="MGDSLKALEDQYMFLTQQLSMFLASCQTQDQRNAIMTQYVMARR
                     NYWNSINKIFHDDDPRIISLVDQMHREQKQIEDCTNHLDKIAQVIDTITEAVTVGTKL
                     AGMAG"
     gene            complement(5411..6667)
                     /locus_tag="VFA02_00680"
     CDS             complement(5411..6667)
                     /locus_tag="VFA02_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZU08389.1"
                     /translation="MTTAAIASEQLTHEQKLDRLAEVAIRVGLGLERGQELVMTASLD
                     ALPLVRRITEHAYRAGASLVTTLFSDDETTLMRYKFAPDESFDRAPAWLYDGMGAAFK
                     SGAARLAVAGANPSLLSNEDPEKVGRANRAVSQAYRPALELITRHEINWTIVACATPA
                     WAAAVFPNDEPAAALEKLWDAIFKASRVDADDPVAAWKSHDATLHKRAGYLNYKRYAA
                     LQYRGPGTDFRLGLAEDHLWLGGGTTAGNGLYCIPNMPTEEVFTTPHKDRADGVVTAT
                     KPLSHQGTMIEGIQVRFEKGRIVEAKATRGQEVLEKLIDTDEGARRLGEVALVPHSSP
                     IASSGLLFLNTLFDENAACHIALGQAYSSCLRDGDKLTQEQLAAKGANSSLIHVDWMI
                     GSDKLDIDGITASGTAEPLMRQGEWV"
     gene            complement(6672..7529)
                     /gene="prmC"
                     /locus_tag="VFA02_00685"
     CDS             complement(6672..7529)
                     /gene="prmC"
                     /locus_tag="VFA02_00685"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZU08390.1"
                     /translation="MNGNLNLKQALAEGAGRLEGASTTPRRDAELLLMRVLGYDRAFL
                     LTHPDATLTAEQSATYENWLRRRAAHEPIQYILGEQEFFGLTFAVTPDVLVPRPETEH
                     LVEAILERVAHNLPLRIADVGTGSGAIAVALAVKLPQAQITALDISEAALTVACRNAQ
                     AHHTAERIRFLLSDLLAAVAGETFDAIVSNPPYVAEADRDSLEPQVRDYEPDAALFAG
                     PSGLDVYKKLIPQAFDRLKPGGWLLLEMGQGQRDAIARLLAGWNNVGFIHDLQGIPRV
                     ACAQRPGLH"
     gene            complement(7545..8762)
                     /gene="hpnI"
                     /locus_tag="VFA02_00690"
     CDS             complement(7545..8762)
                     /gene="hpnI"
                     /locus_tag="VFA02_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZU08391.1"
                     /translation="MVSRHIFDLLLGLGILGLISSTVFQGMVFAGAIRFASERRKRQK
                     AALSEFCPAVSLLKPLHGDEPNLEAHLATFFEQDYPNFEVLFCARNANDPGLLVAKRV
                     AARYPHVKTKFLCTGEQTYINAKVSSLEKMAAAASTDIFIISDSDVRVTPKYIREVIA
                     PFSRENVGAVTCLYRGVADEGLWSKLEAAGMSIEMTSGVLVANMVEGMKFALGPTMAV
                     RRKCVDEMGGFGRLGPYCSDDFLLGNWVAAHGYEVVLSDHVIDHVILNLSFQTSIRHQ
                     VRWMRSTRFSRPKGHFGTSLTFAVLFGCMAFVAALRLDLPHLAWLLLGWSIVARMVMA
                     AVVGSAVIRERHLLRTTLLYPLRDLMGFFFWAASYIDNHVVWRGQVYRLAKDGLMLPA
                     SVREPEREPAVMT"
     gene            complement(8843..10201)
                     /gene="murC"
                     /locus_tag="VFA02_00695"
     CDS             complement(8843..10201)
                     /gene="murC"
                     /locus_tag="VFA02_00695"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZU08392.1"
                     /translation="MFAKAQRVHFIGIGGIGMSGIAEILLNLGYSVSGSDLRRSAITE
                     RLASLGATVFEGHAAANVVGSGVVVTSSAVNERNPEVVEARARKIPVIQRAEMLAELM
                     RLKYGIAVAGMHGKTTTTSMIATVLAAGGLDPTVVVGGRVDALGSNARLGKSQYLVAE
                     ADESDRSFLKLSPILAVVTNLDREHMDCYRDMEDVENAFVEFMDRVPFYGSCVACLDN
                     PQLAAILPRVQRRVFTYGMNPKADFVTRVLADRRFEVAANGQVFGPFELHVPGRHNVL
                     NATAAVAIAAQLEIKPAAILEGLNSFRGVDRRFQIKGCERGVTVIDDYGHHPTEIHAT
                     LQAAKECGYGKVHVIFQPHRFTRTRDLMPEFLTAFEDADSLEVLDIYAASEVPIAGIT
                     SKVLVEGMKHSQAHYAATTEEAITRVVSRANEGDAILTLGAGNVSQLAPLVLEKLKTN
                     AP"
     gene            10394..10735
                     /locus_tag="VFA02_00700"
     CDS             10394..10735
                     /locus_tag="VFA02_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08393.1"
                     /translation="MKRVIGAFILAAATVLPAARTHAQVGISVQIGPPVVAVPAYAPP
                     CPGPGYIWAPGYYAGAVWVPGHWVYRTYYHGPRYYYPYGYYREDHDWYRHDHGHHYGW
                     YKHHDDDDEDR"
     gene            complement(10808..11467)
                     /locus_tag="VFA02_00705"
     CDS             complement(10808..11467)
                     /locus_tag="VFA02_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU08394.1"
                     /translation="MKKALVSWSSGKDSAWALHVLRQSGEYEISGLLTTLNAAFNRVA
                     MHSTRRNVLEAQAAAAGLPLWTVPLPWPCSNELYEQAMSEACKKAVAAGIEVMAFGDL
                     FLEDVRRYREERLAGTGLTPVFPLWGLPTRELAQEMITAGLKARIVCVDPRKLPPEFA
                     GRDFNAALLRELPAGADPCGENGEFHTCVYAGPMFQHEIGIVSGEVVERDGFVFADVS
                     T"
     gene            complement(11464..12618)
                     /locus_tag="VFA02_00710"
     CDS             complement(11464..12618)
                     /locus_tag="VFA02_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZU08395.1"
                     /translation="MAKQNSGFSTRAIHDGQEPDPQTGAVSVPLYLTSTYAQEEVGKH
                     KGWDYSRAGNPTRDALEASLASLEGGANACVFGSGMAAIMALVTLLKSGDHVVCGENV
                     YGGTTRLFNQIVVNYGIEFTYVDTSNPENVRRAIRPTTKLVHIETPTNPLMTLTDIRA
                     VAGVCHEHGIDMSVDNTFMSPYLQRPIELGADIVMHSTTKFLNGHSDGLGGVLVGTRP
                     EHKQQFGFVQKSTGGILSPFECWLVLRGVKTLAVRMKQHDENGRKVAQFLAGHKKIRK
                     VFYPGLPEHPQHELAKKQAYGFGSMMSFETGSLENAKALLRNVRLCTLGESLGGVETL
                     ISHPATMTHAATGAEMRARLGITDGLVRLSVGIEDVDDLIADLDQALAKL"
     gene            complement(12770..13912)
                     /gene="hpnI"
                     /locus_tag="VFA02_00715"
     CDS             complement(12770..13912)
                     /gene="hpnI"
                     /locus_tag="VFA02_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZU08396.1"
                     /translation="MLAIIVESITTILALCGMGFYFVALWSARSFVRRKRVGAEGYCP
                     PVSILKPLKGFDRDMYAGFVSHCRQQYAGEYEVLFGVSGLEDPAVAAVQQLQAEFPEV
                     AIRLVVTPEVLGTNGKVSNLAQMLPQARYGHVLINDSDIRVSPQYLAHVMALFQDPKV
                     GMVTALYRGRSHGTVWSKMEALGISTDFAAGVLTARKIEGGIRFGLGSTLAMSRMALD
                     AIGGLAPLAEYLADDYELGARIAAKGFHVELCDEVVETTVPAYGFRPFWEHQLRWARG
                     MRDARKGGYAGVVFTFGLPWALLNVVASGADLFSIALLSLVFAARVMLALAVGVGVLR
                     DLEVLRDLWLLPLRDALALCVWAWSYAGNTVVWRGQEFGLKNGKLT"
     gene            complement(13947..15218)
                     /locus_tag="VFA02_00720"
     CDS             complement(13947..15218)
                     /locus_tag="VFA02_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08397.1"
                     /translation="MRVTDSREAVRMAIETLRTNKLRSGLTILGIVIGVTTVITISSV
                     INGLNNNVQQWVSSLGTNVLWIFHMPVIGVRPTAEMLARKKLTFDDVAAIRQLPHVVA
                     ADGGVRHVNPIFQAGIVGVKYGTKMAQGTMLQGDSYQLSEVNDVPLTAGRMFTEEEDQ
                     RRANVVVLGHDTAEELFGDENAIGKEVTIENNSFTVIGVLDKFKQAFGGGRNPEDNAA
                     YFPIGTFMKIHPEDATKAGGGVWVSVKYDDARNRSLVEDEVRELLRRRRKVRVEQPDN
                     FEIFGPDSLSRLWNQLTGGLAIFMVAVSSVGLMVGGVGVMNIMLVSVTERTREIGVRK
                     AIGATKRNILLQFTIEAVTLCALGGVIGIFVGGIMTLLIRLLVSALPASMSSLWAIVG
                     FSVSCLIGLVFGIYPAWKAANLDPIEALRYE"
     gene            complement(15215..16441)
                     /locus_tag="VFA02_00725"
     CDS             complement(15215..16441)
                     /locus_tag="VFA02_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08398.1"
                     /translation="MEFIEAIKIALQSLWANKLRSILTLIGVVIGVASVIAVITLTNG
                     VNKYVATKVYSYGADVFTLSKQPPVIFTYEEYEKYQKRKDIHLEDYEAIRDECKMCVD
                     VGALQATTGKVVYGSHSSTDTSIRGWTWNMPAMSNLNVVLGRSFTPSDQQYATHTAII
                     GYDIVDNLMPGMDPLGKEIRIDGEPYTVIGVGEREGKTLGQSQDNYVAVPITTYQRKY
                     GTNASITIYVKAGAVGAALDRASDEARAILRSRRHDAPGADDSFSLDTNATFVGLWTS
                     ISQTFFFVVIGIASISLVVGGIVIMNIMLVSVTERTREIGVRKALGARYTDVLLQFLI
                     ESATMSLIGGAIGVICGVGVAKLVTLAIGFPSDIQIWSVLLALFVSGGVGIFFGVYPA
                     RKAAMLDPIVALRSEL"
     gene            complement(16598..17341)
                     /locus_tag="VFA02_00730"
     CDS             complement(16598..17341)
                     /locus_tag="VFA02_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZU08399.1"
                     /translation="MADAVSQPVGTPLSEGQRIVDTFVAPSKTFTDILRKSSWWGPLL
                     ILIIVGTLFSFAVQTKVGWEKVFENNLHQTPKQEERFAQMPADQAATTRAVFTKVTAI
                     TTYGYWVFALIITAIFSLLTWATVNFGFGGTAKYGQVFAVYMYANLVMNLKYILALVA
                     LFAGLAPDSFLIQNPVGTNIGFYLSTDAPKWLWAFCTHLDVFEIWAVVLTVIGIAIVG
                     KVSRGKAAAAVVGWWVLIVLLLTAVAAAS"
     gene            17577..18389
                     /locus_tag="VFA02_00735"
     CDS             17577..18389
                     /locus_tag="VFA02_00735"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HZU08400.1"
                     /translation="MRCISFFLATALLAGTGTSAFAAAKKPRHRAHTAAARKSVHARH
                     TRHSRRAALEARDDSPKANIPEVPLKHAKLVLLPPLKGSRASLVRQNERAEAEGLERI
                     EEDEQLNELRRDHQLVAVPASMSLRVNPDLPANRRYCRPWTARFLSDLARVHYARFHR
                     PLQVNSAVRTVEYQRHLIEINDNAAPADGDIASPHLTGATIDIAKKGLSLSEIAWMRA
                     YLLPLQTAGKIDVEEEFYQSCFHITVYKSYAPTVKPAQKKHNGSTLLAAGVQ"
     gene            complement(18437..18913)
                     /locus_tag="VFA02_00740"
     CDS             complement(18437..18913)
                     /locus_tag="VFA02_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU08401.1"
                     /translation="MKAKNTVCLWFDKDALDAARFYAATFPQSEVIAVHTAPSDFPGG
                     KKGDVLTVEFTVAGIPCLGLNGGPAFRHSEAFSFQIATDNQEETDRYWNAIVGNGGQP
                     SACGWCKDRWGLSWQITPRALTDALAAGGDEARRAFEAMMTMGKIDIAAIEAARRG"
     gene            19129..20250
                     /locus_tag="VFA02_00745"
     CDS             19129..20250
                     /locus_tag="VFA02_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08402.1"
                     /translation="MKQPRLRGFSLFAIALTLLCFQHRAHAQGCVAAHSEQPVLSELD
                     MDSLPASSGHSPSVISGLSPSSQSSLRGLLRGLSVTVGFRTYSSYKHFVGTVYQAQRA
                     AKHNQVQNHVDLYDLALNYQITPRWSVIADIPAMTATRHQQGNINTYRSGGIGDVLVG
                     AQAWVWRPPTESNGNIAFQMQLKLPTGINDAKGTTILSNGQKQVRPFDESIQPGDGTW
                     GFSLGAEAYRQFWFRTTGYFTGSWLFSPQDTTGVKTYRSAPGEDVISATDQYLWRGGF
                     SHPVPKIRGLALSAGGRMEGVPVRDAFGSSNGFRRPGYVISFEPGLMYAHGRNFFDVS
                     GPWAMERNRKISVTDIENHTHGDAAFSDYTVIASYTRTF"
     gene            complement(20394..20846)
                     /gene="rplI"
                     /locus_tag="VFA02_00750"
     CDS             complement(20394..20846)
                     /gene="rplI"
                     /locus_tag="VFA02_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZU08403.1"
                     /translation="MEVILKEDIQNLGHRGDVVKVADGYGRNYLLPKKLAMEATPANK
                     AVIEQMKAAAVRRSAKEKAEAEQLLTQLNDVALVFERKVGEHDHLFGSVTSADIAQAL
                     EAKGFNIERRKVTLDEPLKSVGEFLIPVKLHREVTAHIKVTVKGEEAA"
     gene            complement(20925..21239)
                     /gene="rpsR"
                     /locus_tag="VFA02_00755"
     CDS             complement(20925..21239)
                     /gene="rpsR"
                     /locus_tag="VFA02_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZU08404.1"
                     /translation="MSDETTQIAPAAAAPRAAAAAQAGAPAAPGARKFFRRKKVCKFC
                     TEKIDSISYRDVNLLKQFVAERGKIVPRRLTGVCTTHQRRLTRAIKQARNIALLPFAV
                     KY"
     gene            complement(21323..21736)
                     /gene="rpsF"
                     /locus_tag="VFA02_00760"
     CDS             complement(21323..21736)
                     /gene="rpsF"
                     /locus_tag="VFA02_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZU08405.1"
                     /translation="MQRFYEVMFIVRPDVQDEDLDKLIAGFEATVTNGGGTVRSTEKL
                     GRRKLAYLVRKFSEGNYVLLTMDADGALVAELERRLRVTEQVIKFITVRMDEEQKRLD
                     KIKAIRATKKKVSPEAPVPAPEAPEAPAEAAQASA"
     gene            complement(21797..22411)
                     /gene="pth"
                     /locus_tag="VFA02_00765"
     CDS             complement(21797..22411)
                     /gene="pth"
                     /locus_tag="VFA02_00765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZU08406.1"
                     /translation="MHVGTGVFLVVGLGNPGIEYQFTPHNAGFLAIDRIADDYGAQVT
                     NRRCRAVTATIRMAGREVVLAKPETYMNLSGISVEALVRELEIDPERDLVVIYDELAL
                     PLGTLRVRTGGSDGGHNGAKSINGALRTQEWARIRIGVGPKPGEMNGRRGRDYLLTPM
                     RKADLAELGPVLDKAAKAVEMIVSQGIAAAMNEFNRREQNGLAE"
     gene            complement(22412..23083)
                     /locus_tag="VFA02_00770"
     CDS             complement(22412..23083)
                     /locus_tag="VFA02_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZU08407.1"
                     /translation="MITQEVVAATPREGKFNKNAARRVRAKGKIPAVVYGAQEPSVAV
                     EVDPKQILRILHSDAGHNSIFDLEIGGNKAKAMIVDWQYEPIKGSLLHIDLKRIAMDK
                     AIRVEVPIQLLGVPVGVKTQGGILDQVLREVEVECLPGDIPSHIDVDITDLAFGDVLR
                     VSDLPHAGKLKFLTDENTTVAHIVAVKEEAPATPEAEAAAAAPVEPEVAKKGKQETAE
                     AEAKK"
     gene            complement(23248..24276)
                     /locus_tag="VFA02_00775"
     CDS             complement(23248..24276)
                     /locus_tag="VFA02_00775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZU08408.1"
                     /translation="MSPETAKETTQQDGRVKPAAERKRSRGLNEDRRFKIFSGSANRE
                     LALEICRHIGVTLGETKMQRFSDGEVYFQLLENVRGVDVFVVQPTCYPVDQHLVELLI
                     MIDALKRASAGRITVVVPYYGYARQDRKDRPRVAISSKLVADLLTTAGANRALFMDLH
                     AAQIQGFFNIPVDHLFASPVMVSYFRELNLPDLTVVSPDAGGVERARHFATKMGVPLA
                     IVDKRRTDINVTEVMHVIGDVQGRTCLIIDDIIDTAGTLIKTVDALLEKGAKKVYAGA
                     SHAVLSGQAVERIANSRLEEVVVTDSIPLSEEAKKVSKIKVRSIAGLLGAAIESIHME
                     TSVSSLFS"
     gene            complement(24431..24504)
                     /locus_tag="VFA02_00780"
     tRNA            complement(24431..24504)
                     /locus_tag="VFA02_00780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24470..24472),aa:Gln,seq:ttg)
     gene            complement(24576..25583)
                     /locus_tag="VFA02_00785"
     CDS             complement(24576..25583)
                     /locus_tag="VFA02_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZU08409.1"
                     /translation="MSTRVRSFCKINLGLAIGPLRPDGFHALTTLYQTLAAHDLVTVR
                     VDEATLASRNLASGTQGEAPTPSRIAITASDERVPTDARNTVWKMLDRVLAAANFSAE
                     VHVHIEKRLPVQGGLGAGSANAIAALIGLERELGMRLPGAERLRIAEEVGSDVPLFLV
                     GGAVLGLGRGEEVCPMPDLPESHCVVALPAVGVSTPQAFRDWDASQEQRLTAGAASDR
                     LKESGRVYSACLSGAFSSGEGQLEDPLLALVRTGIQNDFEEVVFRQYPSLGQIKRILE
                     PGAVYAALSGSGSAIFGLYRTEEAAAEAGKRLSSHGTAFLRTKTLPREAYWKMMLAEN
                     E"
     gene            complement(25660..26496)
                     /locus_tag="VFA02_00790"
     CDS             complement(25660..26496)
                     /locus_tag="VFA02_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08410.1"
                     /translation="MKRFARVPAMAAAFLAMLTMVTGCNRLKARDQLNKGVAAYKNAR
                     YEEAIGHFQNAVNLDPSLPMAKLYLATAYAQQVVPDLKTPENLKNANLAIEGFQDVLK
                     KDPKDVSSLKGIASLYFQIDEFDKAKEWQQKVLAVDPQDAEAAYTIGVIDWGIAHKNQ
                     VEALSAVGLQDQGDGNPKMPKAACQKLAEQNGPLVSEGLEYLQKALTIRPNYDDAMAY
                     MNLMYRQKANLECGNDQARKEDVDQAVQWTQKTMETRKINEQKKNQTPGGIVMDNQGN
                     MK"
     gene            complement(26749..27186)
                     /locus_tag="VFA02_00795"
     CDS             complement(26749..27186)
                     /locus_tag="VFA02_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU08411.1"
                     /translation="MAMGTGSGPGGLSADINVTPLIDVLLVLLIIFMVIVPVTPHGLE
                     AQVPQPPKNPQQQKQNDLNIVVSIIAHGGAQPTYMINQDNVPKSDLQNRLNAIFSIRA
                     EKVIFIKGDADLNYQAVAEVVDMARGLGIDHIGLVTPKIEAGQ"
     gene            complement(27231..27668)
                     /locus_tag="VFA02_00800"
     CDS             complement(27231..27668)
                     /locus_tag="VFA02_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU08412.1"
                     /translation="MALVKRDEGKKVNSNINVTPMVDVMLVLLIIFMVITPMLQNKVN
                     VDMVRTENATAMPDADHEDAVVVAVTRDSKVFLGQNEVSLSDLGSKVGDLLQNKINKM
                     VYFRADARAHYGTVMDAIDAVRTSGVEQVGLLTEQRTNQIGGQ"
     gene            complement(27803..28543)
                     /locus_tag="VFA02_00805"
     CDS             complement(27803..28543)
                     /locus_tag="VFA02_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZU08413.1"
                     /translation="MILAQLTHAVLHPANLAFFQEQTVGFSPLELWGNMGWLARTVVI
                     ILFIESIWSLAVIIDRWLYFGAARKQSREFAPRVAGALKEGKLDEAVKVADRNKKSHL
                     AEVVTAGLQEFRSFGGGGAVNEEQIESSKRALERAEAIVHAKLKRGLGGLATIGATAP
                     FVGLFGTVVGILNAFRQIAVQKTSGIGAVAGGISEALVTTAMGLLVAIPAVMTFNYFT
                     NKVEAFDVEMDNSSSELIDYFLKQSNRR"
     gene            complement(28671..29393)
                     /locus_tag="VFA02_00810"
     CDS             complement(28671..29393)
                     /locus_tag="VFA02_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZU08414.1"
                     /translation="MFEDALMESGGKIKTRSKYFTIIGFVLNGSILAGMILYPLIYPE
                     ALPKAMMSTLLVAPPPPPPPPPPPPPQAPVVHKVVSELVNNQMTFTKIPKEIKMVHEE
                     APPPAAGVAGMEGMGGSSGGVLGGVLGGIGNGPAPVVKAAPPKKIAVSSGVMQGNLLV
                     KTQPQYPAIAKAARIQGTVVLQATISKQGTITNLHVVSGPPMLQQAALDAVKTWRYKP
                     YLLNGEPVEVETQVNVVFTLGG"
     gene            complement(29656..30858)
                     /gene="secF"
                     /locus_tag="VFA02_00815"
     CDS             complement(29656..30858)
                     /gene="secF"
                     /locus_tag="VFA02_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZU08415.1"
                     /translation="MELFHDVKIDWLGKKWYFLGFSLIFSVAGVLSMLFWHHIPLDVD
                     FRGGTVVRVKFDQTPDLDRIRSASDSAGIKDVRIQRYGRPADNEVIISLALSHTSENA
                     LDQGRAAILQALENHYVRNNQANAGKLDLDNAGQGTLANWLTQKDPEHLGQNDAAQQH
                     YSQQAAAVLSYRDEHSGLIGSVDELSGKVSPAVLGALKQDAYLSGFAIRNVEIVGPQV
                     GAQLRNQAMWAILYSLLGMLVYLWFRFELIYGVAAVVAVFHDTLITVGAFSLTNQEIS
                     LTVIAAILTLVGYSMNDTIVVFDRIRENLRMSRREPLADLVNRSINQTLSRTVLTSGL
                     TFLTVLSLYLFGGEVLHGFSFALVVGILIGTYSSIAVAAPMLVAYQDWRQRRGKAAVL
                     PAAKKAKV"
     gene            complement(30931..32550)
                     /gene="secD"
                     /locus_tag="VFA02_00820"
     CDS             complement(30931..32550)
                     /gene="secD"
                     /locus_tag="VFA02_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZU08416.1"
                     /translation="MRKNLTQKTILIIAILLVFLFGIFGIPRGLSGTALKEAILNRIH
                     LGLDLRGGTHLILQVMVDEAVGSQTDNDVARLQTDLQQNGITVGSITKPDSAHPETIL
                     ISGVPADRLGDARSVLNQRYGSQYDISSGENNSFTLTMKPTVVADLKKRALEQAIEVI
                     RTRVDSLGVSEPVIQEYNLGAYQILVELPGVDDPARVRDVIQSTARLELHLVEGGPWP
                     DEQTALQALGGSVPYDSVLMRAIPGAAGPNSNEAVYEIKRVPEVAGTDIRDAQPGRNP
                     NTNEPQVVFYLTAAAGNHFADFTTANKGKPLCVVLDNKIREVATIRDTIRDQGTISGG
                     GITEQYAKDLAMLLRTGALPASIHYLNESVVGPSLGADSIREGVLASVVGMLAVMIFM
                     LIYYRGAGINADLALFLNLVILLGFMGFTHSVLTLPGIAGVILTIGMGVDSNVLIFER
                     IREELRAGKSASAAVDQGFAHAWTTIVDTHVTTIVSAAILFIFGTGPVRGFAVTLTFG
                     LLANLFTAVFVSRVIFDAHLNKKRPGEPISI"
     gene            complement(32557..32718)
                     /locus_tag="VFA02_00825"
     CDS             complement(32557..32718)
                     /locus_tag="VFA02_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZU08417.1"
                     /translation="MKPGDRVTTSGGIRGTILSIKDDAIQLRVPPDNIKMEVVKSAIA
                     SVTTDEPAK"
BASE COUNT         6799 a         9843 c         9076 g         7005 t
ORIGIN      
        1 ttgacgtctg cttcgagcag agcgacgcgg atttcccgca gggcctcacc gatgttttcc
       61 tcggtgacgg tgccctgtcc gcgaaggttt ttgaaggccc gctgaagctt ttctgagaga
      121 ttttcaaaca tgatggcttg ctttcagttt atcagcgggg ccggaagtgc gataataagc
      181 caatcatcgg accgctgacc ggaatttctg cgtacttcag gaactctcca gccggacaat
      241 gaaaaaggag tgtattttat ggctggagac ctgcaactta cgtgcagcga ttgtgggcaa
      301 gagtttactt ttacagaggc cgatcaggcc ttctttcagg aacgcggcta ctctacccct
      361 aaacggtgca agtcctgccg gcaggcgaag aaaagcgact cctacggcgg tgggggacag
      421 cggagctggt ctgctccgca gggtacgccg gtcatctgcg ccggatgcgg acagcagacc
      481 acggtcccgt ttgagccgcg cggcgaccgt cctgtttttt gcaagagctg ctatcaggcc
      541 caggcaggaa accgcggcag acagcagcgc gattcaggac gcagacggta ttagacccta
      601 atttaaaacc atcattcgat aaagcaggtc ccgctgcgcc gggcgaaatc cggcatcacg
      661 gatgatccgg cgcacatcct cttctgtcgc atgggaaggg acgggacccg catcatctgc
      721 gccgaagcgc aaggccattt gcagcatctt cagcccctgt gcggtcgagc ttgcctgaac
      781 atgcggtatc tccggcagat acagacggca gatggccatc attttcaggt actcgacgcc
      841 cgttggggca tccagctcac gcgccggagc atggctacgc ggggccagtg cgataaatcc
      901 gcccgtttct ttctgtacat cactcactct atcgagaaga tccaggcgct gctcaaggcc
      961 ttcacccgca ccaaagatga agtgcgcggt tgtcttcatt tgcgcgcggt gcgcagcgcg
     1021 atgtacagcc agccaatcat ccatggccag ggcctcattg tcggcgatgg aatccagccc
     1081 ggcactacgc aagcgaaata gagcgtcctc caaagaaaaa tggctgcgtc tggccaacag
     1141 caaaatctct gacgcacaca caaggatctg caaagacgga aatttcttcc gcagagcgcg
     1201 cagtgtttct tccaggtctt ccacagaaga ttgcgatgcc agcggaatgc ggatcccgct
     1261 actttcagcc tcggcctggc agcaaccggc atcggtcccg gagaaaatcc tctcgcccag
     1321 agcataggta accacgtcgt ctggatgggc gttcctgcgg gcagcatcgg cttccatacc
     1381 cagaccgatc aggtcgctcg aaagaaaaag atcaagagct tgtttacgtg tcatgcgctt
     1441 tcttttcatt gtatgggctg tgcccgtctc tctgaaatgt tcaaaaaata tctgcatgcc
     1501 acctgtcaca aatgcacagg ccgtctcgtc ctggctgcgg agggaaacgg aagatggaaa
     1561 agtatgtgat cgttggcggc ggaattgcag gattaacagc ggcaaatgct ctggctggca
     1621 atggcgaccg tgtcacggtg ctggagcagg cccatcacct gggcggacgc gcacagaccc
     1681 agcagcagag cgggtatttc atgaaccttg gcccgcacgc gctctattgc ggaggcgtgg
     1741 ccatgcggac cttccgcgaa tggggcatcc ccttttccgg aaactttcct ccccgcggcg
     1801 accgcagtta tttcgtgcgc gaaaacaaac tctatcctgc tgtgcgcgat gtcaccagcc
     1861 tgatgacgac gcggttgttt tctgtgcgtg aaaagctgga ggcagcacgc atcttcagcg
     1921 catttacaaa cgccagcgct gaagaaggtg aaacgatgcg agactggctg gagagacatg
     1981 cccgttctga gcgcgtgcgc gattttgccg cgacgattgc gcggatcgct acctacacaa
     2041 ccgatcttga ccacctgagc gccaaggccg ccgtgaagca ggtgcagatg gcaatcaggc
     2101 acaatgtctg gtatctcaat ggaggatggc agacgctggt ggatggtctg cgtgatcgag
     2161 cacggtcgct tggggttgaa atccgcagcg aagagcatgt gacttcgcta cgccggctcg
     2221 atgccgatgg cattgtgctc gcggttggtc cggccagcgt ggagaagctg acaggtgtgg
     2281 ctgttccgga ccagcggcca ttgcgcatgg caacgttgga tatcggtatg agaagacttc
     2341 ctgagggggc ggcgcatgtg gcttttggac tggaccagcc gctgtatttc tcagtgcatt
     2401 cagagtctgc gcgcgtggcg ccggaaggcc gagcacttgc gcatgtggcg aaatatctgg
     2461 gcgactcgca gcctgacccc aaagccctgc gtgaggagct ggaagactac gcttcactgg
     2521 tcatgcctgg atggcagcgc gaggcagagg tcgtccgctt tcttccgaat ctgactgtct
     2581 cgccgatgat ggtcaccccg caagggcgtc cggaagtaga ttttccggga cttgagaacg
     2641 tgactgtggc cggcgattgg gtaggcacag aaggcatgct ggtggatgcc gctgtggcca
     2701 gcgcgctcaa ggccgcgcga gtgatacaag ggcgaaagac gagagaactg attgcatgac
     2761 gatggaccgc gaagctgtat ttctggagtt aaaacctatg ctcttttctt tggcctatcg
     2821 catgcttggc acgcgcgccg atgcagagga tgtggtgcag gaaacctatc tgcgctggcg
     2881 tgaggctgct gaagacgaga tccgctcgcc tcgggcgtat ctcgctaccg ttgcggcgcg
     2941 gctctctctc gattcactga agtctgcgca gcgcaagcgc gaggtgtaca tgggtacatg
     3001 gctgccggaa ccattggtcg gtcctttggg gcagcaatcg gtggacatgg ccgagtctct
     3061 gtcgctcgcc tttctccatc tgctcgaatc gctttcgcct ccggagcgcg ttgcgttcct
     3121 gctgcacgag attttcgaga tgccttactc ggagcttgct gccgtgctgg aaacctctga
     3181 agcgaactgc cgtcagcttg tgacccgcgc aaagaaacac atccagcaga agaaacggcg
     3241 ctttaccgta gaccgggagc accatcgcaa tgttctggaa aaatttcttc acgcctgcgc
     3301 ttccggcgat ccggcgcaac tggcaaatct gctgcatcag gacgtggtgc tctattccga
     3361 cggcggagga aaggtgcgtg ctgcgctgaa cccgatcttt ggcatggaca aagtcatgcg
     3421 cttctttgcg ggaatcgtga agaagaacgc gcttcgcggg tattctgcgg aactggccga
     3481 agtgaacggc gggcctggtg cggtcttcta taaagatggc gaacttgctc aggtactgga
     3541 tcttgaggtg gacgagtcag gacggatcat caatattttc cttgtctcca atccggagaa
     3601 gctgccgcac gaggtgggcc ccaagctcca gcgatagctg gcaggtatta taaagatata
     3661 catactgcat ggcagaccag cctccaggta cccagcttcc aggctctggg gcagttgcat
     3721 ttctgtcccg cgactttcgt ctttaccaac ttgcgcgcgt actggtcatt attggcgcgg
     3781 aggcgcagac gcttgccatc gcatggctgg tctatcagat tacgaacagc gcgttatatc
     3841 tcggctacac agggttgacg ctgtttctgc ccgggctgct gtttctcctg cctgcgggac
     3901 acgcttcgga ccgctttgac cggcggcagg tcatcattct ctgctatacg ctgcagattt
     3961 tctgtacgct ggcgctgttt ctgttcgcgt ggcatgggct gcaccgggtc tggcctattt
     4021 atgccgtcct tttcttagtt ggcaccggac gggccttcag cggcccggca agctcggctt
     4081 tgattcccca ccttgtgccg aaagagcact tcgtgaatgc cgttacctgg ggagctaccg
     4141 ttttccagat agcgactatc gccggcccgt ctcttggcgg cattctttat accttgccgc
     4201 tgcatggaag catcgccggc gcgtccatgg tctttctctt cactctggca acgctgatcg
     4261 tctttgtctc gctgatgttg tcgctgcatg tccgcccggg ccgcatggag catcgcgctg
     4321 cttccatgga agtcctgctg gccggactgc gctatgtgtg gaagacgcaa cttctgctgg
     4381 ggtctatttc actggatatg ttcgccgtgc tgctgggcgg ggcgacggcg ctgatgccga
     4441 tctttgcgaa ggaaattctg cataccggtc cgcgagggct gggcctgctg cgtgctgctc
     4501 ctgcggtggg ggcgcttact gtttccatgt ggcttacatg gaagcccatc cagcggcgct
     4561 cgggtgcgaa aatgctcatc tgcgtgggga tctttggaac agccacggtg gtgtttgggt
     4621 tgtcgaagag tgtgtggctc tcgcttgcgg cattgttctt tgtgggcgcc tcggacatgg
     4681 tcagcgtggt catccgttct tccattctgc aactcgccac accactggag atgcgcggcc
     4741 gtgtcagcgc cgtgaattca ctctttattg gcgcctccaa tgaattagga gaattcgaga
     4801 gcggccttac tgcttcatgg tggggagcgg tacgcgcggt gatttatggc ggcctcgggt
     4861 cgcttgcaat caccggcttg tggagcatct tcttccccaa tctgcgccgt gctgaccggc
     4921 tcacagcaga agcgctttta caggtggaag aagaagagac cgcgcaggaa gtacgcaggt
     4981 gataaagaat gttccacacc tttgatgtgg tcaccacgga acagggaccc cgattttcca
     5041 acctgggccg gctgtggctt atccggccat gcccgccagc tttgttccta cggtgacggc
     5101 ttcggtaatg gtgtcaatca cctgtgcgat cttgtccaga tggttcgtac agtcctcaat
     5161 ctgcttctgc tcgcggtgca tctggtctac cagcgagatg atgcgcggat cgtcatcatg
     5221 aaaaattttg ttgatgctgt tccagtagtt ccgccgggcc atgacgtact gggtcatgat
     5281 ggcattccgc tggtcctggg tctggcatga tgccagaaac atggagagct gctgtgttaa
     5341 aaacatgtac tggtcttcga gggccttcag ggagtctccc actgtgttcc tccagtgaca
     5401 ggactccagc ttatacccat tctccctgac gcatcagcgg ttcggccgtg ccggaagcgg
     5461 taatgccgtc aatatccagc ttgtccgagc cgatcatcca gtccacgtga atgaggctgc
     5521 tgtttgcgcc ctttgcggcg agctgttcct gggtaagctt gtcgccatca cgcagacagg
     5581 agctgtaggc ctggcccagc gcgatgtggc aggcggcatt ttcatcaaac agcgtattca
     5641 gaaagagcag cccgctggat gcaatcgggg aggaatgcgg cacaagtgcg acttcgccca
     5701 gtcggcgcgc gccttcatct gtatcaatca gtttttccag gacttcctgc cccctggtag
     5761 cttttgcttc gacaatgcgg cctttttcaa agcggacctg aatgccttca atcatcgttc
     5821 cctggtgcga gagcggcttg gttgcggtca caacgccgtc ggcgcggtct ttgtgcggag
     5881 tggtgaagac ctcttcggtc ggcatgttgg ggatgcagta aagaccattg cctgccgtcg
     5941 ttccgccgcc cagccagaga tggtcctcgg ccagtcccag gcggaagtct gtgccggggc
     6001 cgcgatattg cagcgccgcg tatcgcttat agttcagata gccggcgcgt ttgtgcagcg
     6061 tggcatcgtg tgacttccat gcggccactg gatcatcggc atctactcgc gatgccttga
     6121 agatggcgtc ccagagtttt tcgagggccg ctgcgggttc gtcattcggg aagacggccg
     6181 cggcccatgc aggcgtggcg caggccacga tcgtccagtt gatttcgtgg cgcgtaatca
     6241 attcgagcgc gggacggtat gcctgcgaaa cagcgcggtt cgcgcgcccg actttctccg
     6301 gatcttcatt cgagagcagc gatgggtttg ccccggcaac cgcaagcctt gcagcgccac
     6361 ttttgaaggc cgcgcccatt ccgtcataga gccatgcggg agcgcgatca aaggactcgt
     6421 ccggggcgaa tttgtaacgc atcagcgtgg tctcgtcatc gctgaaaagc gttgttacca
     6481 gtgaagcccc tgcgcggtag gcatgctcgg ttatgcggcg gacaagcggc agcgcatcaa
     6541 gcgacgcggt catgaccagt tcctggccgc gttcgaggcc gagtccaacg cgaatggcga
     6601 cttcggcaag gcggtcgagc ttctgttcat gcgtgagctg ttcggaggca attgcggctg
     6661 tggtcatgtc tttagtgtaa gcctggccgc tgtgcgcagg ccacgcgtgg gatcccttgc
     6721 agatcatgga tgaagcccac gttgttccac cctgcgagga gccgcgcaat cgcatccctc
     6781 tggccttgtc ccatttccag cagcagccat ccgccgggtt tcagccggtc gaatgcctgt
     6841 ggaatgagct ttttgtacac atcaaggccg gatggcccgg caaagagcgc agcgtcaggt
     6901 tcgtaatcgc gcacctgagg ctccagtgag tcgcggtcgg cttctgcaac atagggcggg
     6961 ttagagacga tggcgtcaaa agtctctccg gcaactgcgg ccagaaggtc ggacaagaga
     7021 aagcggatgc gctctgcggt atggtgcgcc tgggcgttgc ggcaagccac ggtcagggct
     7081 gcttctgaga tgtccagtgc ggtgatttgc gcctgcggca gctttacggc aagtgcgacg
     7141 gcaatcgcgc cagagcctgt gcccacatcg gcaatgcgca agggcaggtt gtgcgccact
     7201 cgctccagta ttgcttccac aaggtgctcg gtttcagggc gtgggacgag cacatccggg
     7261 gtgaccgcga aagtaagtcc gaagaactcc tgctcgccga gaatgtactg gattggctcg
     7321 tgcgcggcgc ggcgtcgcaa ccagttttcg tatgtcgccg actgttcggc ggtgagcgtg
     7381 gcatcaggat gtgtgagcag aaatgcgcgg tcatatccca gcacccgcat caggagaagt
     7441 tcagcgtcgc gtcgcggtgt cgtcgaagcg ccttcgagtc tgcccgcgcc ttcggcaagg
     7501 gcctgtttca gattgagatt gccgttcaaa ttttccggtt gcacttatgt catgacggcc
     7561 ggttctctct ccggctctct tactgaagcg ggcagcatca ggccatcttt tgccagacgg
     7621 tagacctgtc cacgccatac tacgtggttg tcaatataac tcgcggccca gaagaagaag
     7681 cccatcaggt cgcgcagcgg atacagcagc gtggtgcgaa gcaggtggcg ctccctgatg
     7741 acggctgagc caaccactgc cgccatcacc atacgggcca cgatgctcca gcccagcagc
     7801 agccacgcga gatgaggcag gtccaggcgc agcgcagcaa caaacgccat gcatccaaac
     7861 aggacagcaa aggtaagcga ggtcccgaag tgacccttcg ggcgcgagaa gcgggtggac
     7921 cgcatccatc ggacctggtg cctgatggaa gtctggaaac tcaggttcag aattacgtga
     7981 tcaatcacat ggtctgacaa cacaacttca tatccatggg cagccaccca gttgcccagc
     8041 aggaaatcat cggagcaata tgggcccagg cggccaaagc cacccatctc atctacgcat
     8101 ttacgccgca cggccatcgt agggccgagg gcgaacttca tgccctccac catgtttgcg
     8161 accagcacgc ctgaggtcat ttcaatggac atacccgcgg cctccagttt ggaccacaag
     8221 ccttcgtcag caacaccgcg atagaggcag gtgactgcac ccacattttc gcgtgaaaaa
     8281 ggcgctatga cttcgcggat gtacttcggg gtgacgcgca catcgctgtc actgatgata
     8341 aagatgtctg tgctggcggc agccgccatc ttttccagcg aagagacctt ggcgttgatg
     8401 taggtctgtt ctccggtgca gagaaattta gttttaacat gcgggtagcg cgccgccacg
     8461 cgcttcgcca ccagcaggcc cgggtcattt gcattgcgcg cgcagaaaag aacttcgaag
     8521 ttgggatagt cctgctcaaa aaacgtagca agatgcgctt ccagatttgg ctcgtcccca
     8581 tgcagaggct tgagcagact caccgcagga caaaactctg acagcgcagc tttttgccgc
     8641 tttcgccgct cgcttgcaaa gcgtatcgcg ccggcgaaca ccattccctg gaaaacggtc
     8701 gaactgatga ggcccagaat cccaagcccc agcagaagat cgaaaatatg tcttgaaacc
     8761 actgtattca gcgtacagca gagtatgacc ttacaggcca atgaatacat tcagaaataa
     8821 tatcttcttt gatgtcaatg catcagggcg cgtttgtttt caacttttcc agcaccagcg
     8881 gtgccaattg cgacacattt cctgctccca gcgtcaggat ggcatcgcct tcgttcgcac
     8941 gcgatacgac gcgggtgatg gcctcctctg tggttgctgc gtagtgtgcc tgagaatgct
     9001 tcatgccttc gacaaggact ttggatgtga ttcctgcaat cggcacctca ctggctgcgt
     9061 agatgtcgag cacttcgagt gaatctgcgt cttcaaatgc ggtaagaaac tccggcatta
     9121 aatcgcgggt acgcgtaaag cggtgcggct gaaaaatgac gtgcactttg ccatatccgc
     9181 attctttggc cgcttgcagg gtagcgtgta tttctgtagg gtggtggccg taatcgtcaa
     9241 tcacggtcac gccgcgctcg cagcccttga tctgaaagcg gcggtctact ccgcgaaaac
     9301 tgttcaatcc ttccagaatg gctgctggct ttatctcaag ctgtgcagca atggccacgg
     9361 ccgctgttgc gttcagaacg ttgtggcgtc cggggacgtg aagctcaaat gggccgaaga
     9421 cctgcccgtt tgctgcaacc tcgaagcgcc tgtcagccag aacacgcgtt acaaaatcgg
     9481 ccttcggatt cattccataa gtgaagacgc gccgctgcac gcgcggaaga atggcggcga
     9541 gttgcgggtt gtccagacag gcaacacaac ttccgtagaa gggcacgcgg tccatgaact
     9601 cgacgaaggc gttctctacg tcctccatgt cgcggtagca atccatgtgc tcgcggtcca
     9661 ggttcgtgac aacagcaaga ataggagaca gcttcagaaa agagcggtcg ctctcatcgg
     9721 cctctgcgac cagatattgc gatttgccca ggcgggcgtt cgatcccaac gcatccacgc
     9781 ggcctccaac cacgactgtc gggtccagtc cgcctgcggc cagcacggtt gcaatcatgg
     9841 aggtcgtcgt cgttttgcca tgcattccgg caacagcgat gccgtacttc aggcgcataa
     9901 gttcagcgag catctcagcg cgttggatca caggaatctt acgcgcgcgc gcctccacca
     9961 cttccggatt gcgctcattc acggcggagc tggtgacgac cacacccgag ccgacgacat
    10021 tcgccgccgc gtggccttca aagaccgtcg cgccaaggga cgccagccgc tctgtgatgg
    10081 cagaacggcg gaggtctgag ccggaaacag aatagccgag attgagcagg atctcggcta
    10141 tgccactcat gccgattccg ccaataccga tgaagtgtac ccgttgtgcc tttgcgaaca
    10201 ttttttcaga atgctgtgat tactgtatca gcggggctga atgcgatgcg ctgtaagcga
    10261 aaaggtttcc tgcctgggta aaaattttgt tgcgaatgta ccgggaaatc accgcaacat
    10321 cccgcagcat tttccgtttc ttcttcgtgt aagcaaagcg ccggaaatca ccaggcgcga
    10381 ggaggtataa aaaatgaaac gggtcattgg tgctttcatc ctggctgctg ctacagttct
    10441 tcccgctgcg cgtacccatg cgcaggtggg catctcggta cagataggcc ctccggtggt
    10501 cgcagtaccg gcctatgctc cgccgtgtcc ggggccggga tatatctggg cccccgggta
    10561 ttacgccggc gctgtctggg tgcctggaca ctgggtctat cgcacgtact accatgggcc
    10621 gcgttattac tatccatacg gctactaccg agaggaccat gattggtatc gtcatgatca
    10681 cggccatcac tacggctggt acaagcacca tgacgacgat gatgaggacc ggtaaggact
    10741 ggcctcagtc atttgcgcaa ttcagttgcc tcatcctttc ggcctgtttg gaccgaaagg
    10801 atgagagtta tgtgctgaca tcggcaaaaa caaacccgtc acgctccacc acttcgccgc
    10861 ttacaattcc aatctcgtgc tggaacatag gtcctgcata cacgcaggta tggaactcgc
    10921 cgttttcacc gcacgggtct gccccggcag gcaattcgcg caacagagcg gcgttaaaat
    10981 cgcgtccggc aaactcaggc ggcaattttc gagggtccac gcagacgatc cgcgccttca
    11041 gaccggcagt gatcatttcc tgcgcaagct ctcgcgtagg gaggccccag agtggaaaca
    11101 caggagtcag ccctgtacct gccagccgtt cttcacggta gcgccgcacg tcttcgagga
    11161 agaggtcgcc gaaggccatg acctcgatgc ctgctgcaac agcctttttg caggcctcac
    11221 tcattgcttg ttcatagagt tcattggaac agggccaagg caagggaaca gtccacagcg
    11281 gcagacccgc ggccgcggct tgtgcctcca gcacattgcg acgcgtgctg tgcatggcca
    11341 cgcggttgaa tgctgcattg agagtcgtca gcagtccgga gatttcgtat tcgccagact
    11401 gccggaggac gtgcagcgcc caggcgctgt ccttaccgct gctccaggag acaagggcct
    11461 ttttcacagt ttcgccaggg cctggtcgag atcggcgatg agatcgtcta cgtcctctat
    11521 tccgacggag aggcggacca ggccatccgt gatgccgagc ctggcgcgca tctctgcgcc
    11581 ggtggccgca tgcgtcatgg ttgcgggatg cgaaatcagc gtttcgacgc cgccgagcga
    11641 ttcgcccaac gtacacaggc gcacattcct gagcagcgcc ttcgcgtttt ccaacgatcc
    11701 tgtctcaaac gacatcatcg aaccaaaccc ataagcctgc ttctttgcca gctcgtgctg
    11761 cggatgctcc ggcagtccgg gataaaagac cttcctgatt ttcttgtgtc cggcaagaaa
    11821 ttgggccacc ttgcggccgt tttcatcgtg ctgcttcatg cggacggcga gcgtcttcac
    11881 tccgcgcagg acgagccagc actcaaacgg cgagaggatg ccgcctgtag acttctgcac
    11941 aaagccaaat tgctgcttgt gctctggccg tgtgcccaca agcactccgc caagcccgtc
    12001 actgtggcca ttgaggaact tcgtcgtaga gtgcatgacg atgtctgcgc caagttcgat
    12061 gggccgctgc aaatatggcg acatgaaggt gttgtccacg ctcatgtcaa tgccgtgctc
    12121 gtggcagacg ccggcgacag cgcgaatgtc ggtcagggtc atcagcggat tggtcggcgt
    12181 ttcgatatgc acgagcttcg ttgtcggccg gatggcgcgg cggacattct caggattgga
    12241 tgtgtccacg taagtgaact cgatgccata gttcaccaca atctggttga agaggcgcgt
    12301 cgtgccgcca tagacattct ctccgcagac cacgtggtcg cccgacttga gcagcgtcac
    12361 caaggccatg atggccgcca ttccgctgcc aaaaacgcag gcattcgcgc cgccttcaag
    12421 cgaggcgagg ctggcttcca gcgcgtcgcg tgtgggatta cccgcgcgcg aatagtccca
    12481 tcctttgtgc ttgccgacct cttcctgtgc ataagtagag gtaagataga gcggcacgct
    12541 caccgccccc gtctgcgggt caggctcctg cccatcgtga attgcgcgcg tagaaaaacc
    12601 agaattttgt tttgccatag ccgttgttgc tctattttat tcgggtgaga agaagcgtac
    12661 aaaaaacgct ataaacggac ccttcatcgc cttccccgct tctgaggatg acagaagaac
    12721 tggcgagatt tacatttttg cgccctgcga ccttcgtcct gagaccgggc tacgtcagct
    12781 tgccgttctt cagcccaaat tcctgaccgc gccaaacaac cgtgttcccc gcgtaactcc
    12841 acgcccagac gcacagggca agggcgtccc gtaacggaag cagccacaga tcgcgcagta
    12901 cttccagatc gcgcagaacc cccactccga cggccagtgc cagcatgaca cgagcggcaa
    12961 acaccaggct caagagggca atgctgaaga ggtccgcgcc cgaagcaacc acgttcagga
    13021 gcgcccatgg aagcccgaag gtgaagacca ctccggcata tccgcctttg cgtgcatcgc
    13081 gcattccgcg cgcccagcgc aactggtgct cccagaacgg gcggaagccg taagcgggga
    13141 ccgtggtttc cacaacctca tcgcacagtt ccacgtggaa gccttttgca gcgatgcgcg
    13201 cgcccagctc atagtcgtct gccagatact ctgcgagcgg cgcaagcccg ccaatcgcat
    13261 cgagggccat gcgcgacatg gccagcgtgg aacccaaacc aaaccgaatg cccccttcga
    13321 tttttcgcgc cgtcaacact cccgccgcaa agtccgtcga aatgcccagc gcttccatct
    13381 ttgaccagac cgttccatgc gaacgcccac gatagagcgc cgtcaccata cccaccttcg
    13441 ggtcctgaaa cagcgccatc acatgcgcca gatattgcgg agaaacccgg atatcgctgt
    13501 cattgatcag cacatggccg tagcgcgcct gcggaagcat ctgcgccaga ttgctgactt
    13561 tgccgttcgt gcccagcact tccggagtga ctaccaggcg gatggcaact tccggaaatt
    13621 ccgcctggag ctgttggacg gcggcgaccg cagggtcttc caggccactg acgccgaaaa
    13681 ggacctcgta ttcgcccgca tactgctgcc ggcaatggct gacaaaaccc gcatacatgt
    13741 cgcgatcaaa gcccttgagt ggcttcagaa tgctgacggg aggacaatat ccctctgcac
    13801 cgacgcgctt tctgcgcaca aaggagcggg cgctccacag cgcaacgaaa taaaatccca
    13861 tcccgcagag cgcaaggatg gtcgtgatgc tttcaacaat gatggcgagc atgaatgctc
    13921 agtttacaag gacagggcgc attgccctac tcatagcgca gggcctcaat gggatcgagg
    13981 tttgccgcct tccacgccgg ataaatccca aacaccagcc caatcaggca ggacacgcta
    14041 aaccctacga tggcccacag gcttgacatg cttgcaggca gagcgctcac cagcagccgt
    14101 atcagcaagg tcatgattcc gccaacgaag atgccaatga ctccgccaag agcgcagagc
    14161 gtgacggcct caatcgtgaa ctgaagcagg atgttccgct tggtagcgcc aattgccttg
    14221 cgcacgccaa tctcgcgcgt gcgctcggtg acagatacga gcatgatgtt catcacacct
    14281 acgccgccta ccataaggcc cacgctcgag acggcgacca tgaaaatggc gagtccaccg
    14341 gtaagctggt tccacaagcg tgacaatgaa tccgggccaa agatctcgaa attatccggc
    14401 tgttcgacgc ggacctttcg ccggcggcgc agcagctcgc gcacttcgtc ttccaccaga
    14461 ctgcggttgc gcgcgtcatc atatttcacg ctgacccaca cgccgccgcc ggcttttgtg
    14521 gcatcttccg gatggatctt cataaacgtg ccaatgggga aataggccgc gttgtcttcc
    14581 gggttgcgtc cgccgccaaa ggcctgcttg aacttgtcca ggaccccaat cacggtgaag
    14641 gaattgttct caatggtgac ctctttgcca attgcgttct cgtcaccgaa aagctcctcc
    14701 gctgtatcat gccccaggac aacgacattt gcgcgccgct ggtcttcttc ctcagtaaac
    14761 atgcggcctg cggtgagggg cacatcattt acttcgctca gttggtaact gtcaccctgc
    14821 agcatcgtgc cctgggccat tttcgtccca tacttcacgc cgacaatccc ggcctgaaaa
    14881 ataggattca catgccggac accgccatcg gcggccacca cgtggggcaa ttgccggatg
    14941 gcggcaacat catcaaacgt cagcttcttg cgtgccagca tctccgccgt cggacgcaca
    15001 ccaatcaccg gcatgtgaaa aatccacaga acattggtgc ccagcgagct tacccattgc
    15061 tggacgttat tgttgaggcc attgatgacg gaagaaatcg taatgactgt ggtaacgccg
    15121 atcacgatgc ccagaatggt gaggccagag cgcagtttat tggtacggag cgtttcgatg
    15181 gccatgcgca cggcttcacg tgaatctgtg actctcatag ctctgacctc aacgccacga
    15241 tcgggtccag catcgccgcc ttacgggcgg ggtagactcc gaagaaaatc cctacgccgc
    15301 cggagacaaa cagcgccagc agcaccgacc atatctgaat gtcggacgga aacccgatgg
    15361 ccagcgtcac cagcttggcc acgcccaccc cgcagataac gccaatcgcg ccacctatca
    15421 gagacatcgt tgccgactca atcagaaatt gcagcaggac atcggtatac cgcgcgccca
    15481 gggccttgcg cacgccaatc tcgcgcgtcc gctcggtcac gctcaccagc atgatgttca
    15541 tgatgacgat gccgccgaca accagcgaaa tcgaggctat cccaatgact acaaagaaaa
    15601 acgtctggct gatgcttgtc cacaggccga caaatgtggc attggtatcg agggaaaaac
    15661 tgtcgtccgc gcccggagca tcatgccgcc gcgagcgtag aatcgcccgc gcctcgtcgg
    15721 aagcgcggtc cagggcggcc cctacagcgc ccgccttcac ataaatcgta atgctggcat
    15781 tcgtgccgta tttgcgttgg taggtggtga tgggcacggc tacgtagttg tcctggcttt
    15841 gccccagggt cttcccctcg cgctcgccga cgccaatcac ggtataaggc tcgccgtcta
    15901 tgcggatttc cttgcccaga gggtccatcc ccggcatcag attgtctacg atgtcatagc
    15961 cgataatggc ggtgtgcgtg gcgtattgct ggtcagaggg agtaaagctg cgtcccagca
    16021 cgacattcag gtttgacatt gcaggcatgt tccacgtcca gccgcgaatg ctcgtgtcgg
    16081 tgctggaatg cgacccgtaa accactttgc cggtcgtggc ctgcaacgcg ccaacatcta
    16141 cgcacatctt gcactcatct cgaatggctt cgtagtcttc gagatggatg tctttacgct
    16201 tctgatactt ttcgtattcc tcgtaagtga agatcaccgg aggctgctta ctcagcgtaa
    16261 agacgtctgc cccatagcta tagacctttg tggcgacata cttgttcacg ccattggtca
    16321 gagtgatgac agcaatgacc gaagccaccc caatgaccac gccaatcagc gtaagaatgg
    16381 agcgcagctt gttcgcccag agcgattgca gcgcgatctt aatggcttca ataaattcca
    16441 tgctttcaat acttctataa atatcaaccc gatggcgagt tattttgtca tactgaagcg
    16501 ccagccgaaa gacctgctca actttaaagc atacgtccgc ccagccggag aaattctcct
    16561 tcgctgaacg atacgcagaa aaggaagggc cggttcccta cgatgccgcc gccacggcgg
    16621 tgagcagaag tacgatcaat acccaccaac ccacaacggc tgccgccgcc ttgccgcgag
    16681 aaactttccc cacaatggca atgccgatga cagtcagcac aactgcccat atttcaaaga
    16741 catccagatg ggtgcagaat gcccaaagcc atttcggcgc gtcggtagac aggtaaaagc
    16801 caatgttcgt gcccacaggg ttctgtatca gaaacgaatc cggtgccaga cctgcaaaga
    16861 gagcgaccaa cgccaggatg tatttgaggt tcatcaccag gttggcatac atatagacgg
    16921 cgaagacctg cccgtatttc gctgtccctc caaaaccgaa gttcaccgtc gcccaggtca
    16981 gaagcgaaaa aatcgccgta ataatcagcg caaagaccca gtagccatag gtcgtgatgg
    17041 cagtcacttt ggtgaaaact gcgcgcgtgg ttgcggcctg gtccgcaggc atctgagcaa
    17101 accgctcttc ctgcttcggg gtctggtgca gattgttctc aaagaccttt tcccatccca
    17161 cctttgtctg caccgcaaaa gaaaacagcg tgcccacaat gatgagaatg agcagcgggc
    17221 cccaccacga ggacttgcgc agaatgtcag taaatgtctt tgacggagcc acgaacgtat
    17281 ctacaatgcg ctgcccttcg ctcaggggtg taccgaccgg ctgggaaact gcgtctgcca
    17341 taaaaacctc acaatctaca acgattttcc aatggcgcga ttgtatcgtg aagcctggcg
    17401 tctggcgatg aaaatcctaa ggtgaagcgg ccagcacctg ttttgcaggc cggattgcca
    17461 caggaaaaat gctatggccg gggcagcggg tgcgccggaa ccaaactcaa tacttccggc
    17521 ccgggtccat ctccggtagt ctgaaaaagc tctcggaacc tccgcggtcc catccaatgc
    17581 gttgtatctc ttttttcctt gctaccgcgc tgctcgcggg cacgggaaca tctgcctttg
    17641 ccgccgcaaa gaagccgcgc catcgcgcgc ataccgccgc agcgcgcaag tctgtccatg
    17701 caagacacac ccggcattcc cgccgcgccg cccttgaggc ccgcgacgat tcgcccaagg
    17761 ccaatattcc ggaagtgccg ctgaaacatg caaaactggt gctgctgccc ccgctgaaag
    17821 gctcgcgcgc atcgcttgtc cgccagaatg aacgcgcgga ggcagagggg ctggagcgca
    17881 ttgaagagga cgaacaactg aacgagttgc gccgcgatca ccaactggtc gccgtgcccg
    17941 caagcatgtc gctacgcgtc aaccctgacc tgcctgcaaa ccgccgctac tgccgtccct
    18001 ggacggcacg ctttctgagc gatctggccc gcgtgcatta cgcgaggttc catcgcccgt
    18061 tgcaggtaaa ctccgccgtg cgcacagtgg aataccagcg ccatctgatt gaaatcaacg
    18121 acaatgcagc gcctgcggat ggagacatcg cttctccgca tctgaccggg gcgacgattg
    18181 atattgccaa gaagggactt tctctctcag agattgcctg gatgcgcgct tatctgctgc
    18241 ctttgcagac cgcaggcaag attgacgtgg aagaggagtt ttatcaatcg tgcttccata
    18301 tcaccgtgta caaatcgtac gcgccgacgg tcaagccggc acagaagaag cataacggca
    18361 gcactctgct ggctgctggc gtgcaataac gaagaaggtt cgccggcaaa ttcttcggcc
    18421 aatccagtgc ggcgagtcag cctcgccgcg ctgcttcgat ggcggcaata tcaatcttcc
    18481 ccatcgtcat catggcctcg aaagcacgcc tggcctcatc accaccagct gcaagcgcat
    18541 ctgtcagcgc gcggggcgta atctgccagg aaagccccca gcggtctttg caccagccgc
    18601 acgcgcttgg ctgaccgcca ttgccgacaa tcgcattcca gtaccggtcg gtctcttcct
    18661 gattgtctgt cgcaatctgg aaggaaaatg cttcgctgtg cctgaatgcc gggccgccat
    18721 tgagcccgag gcagggaatg cctgcgactg tgaactcaac cgtcaacaca tcgcctttct
    18781 tcccgcccgg aaagtctgac ggagcggtat ggaccgcgat gacctcgctc tgaggaaaag
    18841 tggccgcgta gaagcgcgca gcatccagcg catctttgtc gaaccagaga caaaccgtgt
    18901 tctttgcctt catattcgtg ttctccatat gctccatgag aaatgcaaaa agccgctttg
    18961 cgtgcccggc agcccattcc ccgaattcta tcccagccaa tccctgcctt gcgccggatg
    19021 ctccgccaaa aggctgactc caaaatctac ccagagactg aagaccactc ccatcccgcg
    19081 ctgttactgt gcaatcagtt ttccgaggcc atttctggag caacacacat gaaacaaccc
    19141 cgcttacgag ggttttctct ctttgccatc gcactcacgc ttctttgctt tcaacaccgc
    19201 gcccatgctc agggatgcgt ggcagcacac tctgaacagc ccgtcctctc tgaactggac
    19261 atggactctc tgccggcaag cagcggacat tcgccgtcgg tgatttccgg actcagcccc
    19321 tcgagccaat cgagcctgcg cggccttttg cgcggactct ccgttactgt tggctttcgc
    19381 acctatagct catacaaaca ttttgtcggt acggtctacc aggcgcagcg cgcggcaaag
    19441 cacaaccagg tccagaacca cgtagacctg tacgaccttg ctttgaatta ccagatcact
    19501 ccgcgctgga gcgtcatcgc cgatattcct gcaatgaccg caacccggca ccagcagggc
    19561 aatatcaata cttaccgctc gggcggaatt ggcgatgtgc tggtcggcgc gcaggcgtgg
    19621 gtgtggcgcc ctccaacaga gagcaacggc aacatcgcct tccagatgca actcaaattg
    19681 cccaccggga tcaacgatgc gaagggcacg accattcttt ccaatggaca gaagcaggta
    19741 cgtcccttcg acgaatccat ccagccgggg gacggcacat ggggcttttc gctgggagcg
    19801 gaggcataca ggcaattctg gttccgcacg acaggatact ttaccggatc atggctcttt
    19861 agcccgcagg acacgaccgg cgtgaagacc taccgatcgg ccccgggaga agatgtcatc
    19921 tctgcaaccg accagtatct gtggcgaggc ggattctcgc atcctgtccc gaagattcgt
    19981 ggcctcgcct taagtgccgg gggccgtatg gaaggggtgc ccgtgcgcga tgccttcggc
    20041 agcagcaatg gcttccggcg tccgggatat gtgatttcat ttgaaccggg attgatgtac
    20101 gcccatggac gcaacttctt cgacgtaagc ggcccctggg ccatggagcg caaccgcaaa
    20161 atcagcgtga cagacattga aaatcatacg catggcgatg ccgcgttttc agactacaca
    20221 gtgatcgcca gctatacacg tacattctga tctcctgtgg gatacagaaa gaaacatggg
    20281 tgccccatgt ctcgaaaggg acatggggct tttactggcg agcatttttc gtttcacttt
    20341 gagcagcgga caaggaccat caaacaagct gacagcgccg agggcgcgct gccttaagct
    20401 gcttcttcgc ccttgaccgt cactttgatg tgcgcagtga cttcgcggtg cagcttcacg
    20461 ggaatcagga actcgccgac cgacttcagc ggctcatcga gcgtgacctt gcggcgttcg
    20521 atgttgaagc ccttcgcttc gagggcctgt gctatgtccg ccgaggtgac cgagccgaag
    20581 aggtggtcgt gctcaccgac cttgcgttca aagaccagcg caacatcgtt caactgcgtg
    20641 aggagctgtt cggcctcggc cttttccttt gccgagcggc gcacggcagc agccttcatc
    20701 tgctcgatga cggccttgtt ggccggggtc gcttccatgg cgagcttttt gggcagcagg
    20761 tagttgcggc catagccgtc ggccactttc accacgtcgc cgcgatggcc caggttctgt
    20821 atgtcttctt tcagaatgac ttccattgca aaatctccaa ataacgctga cagccatggt
    20881 gccttctaca agccttctgt tggcttgcat ggaagggccg cggcttagta ctttactgca
    20941 aatggcagca gggcgatgtt gcgcgcctgc ttaatggccc gcgtcaggcg gcgctgatgc
    21001 gtggtgcaca cgcccgtcag gcggcggggt acgatcttgc cgcgctcggc aacaaactgt
    21061 ttcagcagat tgacgtcacg gtatgagatc gagtcaattt tttcggtgca gaacttgcac
    21121 acctttttgc ggcggaagaa cttccgcgct ccgggagcag ccggggcccc agcctgagca
    21181 gcagcggctg cacgaggcgc cgccgctgcg ggtgcaattt gtgttgtttc gtcagacata
    21241 attcctatcc tttttgcctg ccttcaggca atgtaaaaat ctgtatggca cgccaaaacc
    21301 acccggggcg tcagccggaa gattaagcgc tggcctgggc cgcttcagcc ggggcctcag
    21361 gagcttccgg ggccgggact ggggcttccg ggctgacctt cttctttgtg gcgcggatgg
    21421 ccttgatttt gtccaggcgc ttctgctctt catccatgcg cacggtgatg aacttgatca
    21481 cctgttcggt gacccgcaga cggcgttcca gctcggcaac cagcgcgcca tcggcatcca
    21541 tcgtcagcag gacgtagttg ccctcgctga atttacgcac caggtacgcc agcttgcggc
    21601 gacccagctt ttcagtggaa cggacggtcc cgccgccatt ggtcaccgtg gcttcaaagc
    21661 ctgcgatgag cttgtccaga tcttcgtcct gcacatcggg ccggacgata aacatcactt
    21721 cataaaaacg ttgcattgtt tctctcttct cttgaacgcc gcggaggctg tttgcaaccg
    21781 tggccgttac agattcttat tccgccaggc cgttttgctc acggcggttg aactcattca
    21841 tcgcagccgc aattccctgc gacacgatca tctcgactgc tttggccgct ttgtccagta
    21901 ccggaccaag ctcggccagg tctgctttcc gcatcggcgt cagcaggtag tccctgccac
    21961 ggcgaccgtt catctcaccc ggctttggcc caacgccaat ccgaatgcgc gcccattcct
    22021 gcgtccgcag tgcgccgttg atggactttg cgccgttgtg tccgccgtcg ctgccgcccg
    22081 tccgcacgcg aagcgttccc agtgggagcg ccagttcgtc gtaaatgacc accaggtcgc
    22141 gttcgggatc aatttccagt tcccgcacca gcgcctcaac cgaaatgccg ctgaggttca
    22201 tgtacgtctc cggtttggcc agcactactt cgcgtcctgc catccggatg gtcgccgtca
    22261 ccgcccggca ccgccggttt gtcacctgcg cgccgtagtc atcggcgatg cggtcaattg
    22321 ccagaaaccc ggcgttatgc ggcgtgaact ggtattcaat cccggggttc ccaagaccca
    22381 ccaccagaaa cacgcccgtt cccacgtgca attacttctt cgcctcggcc tcggccgttt
    22441 cctgcttgcc cttcttcgcc acttccggtt ctaccggagc agcagccgcg gcttcagctt
    22501 caggcgtcgc cggggcctct tccttcacgg ccacaatgtg ggcgaccgtc gtattttcgt
    22561 cggtcaggaa cttcagcttg cctgcgtgcg gcaggtcaga gacgcgcagc acatcgccaa
    22621 aggcaaggtc cgtgatgtcc acatcaatat ggctgggaat gtctccgggc aggcactcga
    22681 cttcgacttc acgcagcacc tggtccagaa tgccgccctg ggtcttgacg ccgacgggca
    22741 cgcccagaag ctgaatcggg acctcaacgc ggatggcctt gtccatcgcg atacgcttca
    22801 ggtcaatgtg gagcagggag cccttgatcg gctcatactg ccagtccaca atcatggcct
    22861 tggccttgtt gccgccgatt tccagatcga agatcgagtt gtggcccgca tccgagtgca
    22921 gaatgcgaag aatctgcttc gggtccactt cgacggccac cgaaggctcc tgcgcaccat
    22981 acaccactgc aggaatcttt cccttcgcgc gcacgcgacg ggccgcattc ttgttgaact
    23041 tgccctcgcg gggcgttgct gctactactt cctgtgtaat cattctgtcc ttttccttcg
    23101 ggcacggaga aacagcttgg cttttggcct tgagcttctt cttgccgctc cctttgagcg
    23161 cccgttaccg ctcgttcgat tctttcagtt cctctaacgg atgaggaacc acaaaccttc
    23221 catcagcgca tacacgccag aagccagtta actgaacaac gaactgacgc tcgtctccat
    23281 gtggatgctc tctatggccg ctcctaacag cccggcgatc gagcgcacct tgatcttcga
    23341 gactttcttt gcctcttcgc tcaaagggat cgagtcagtg acgacgacct cttcgagcct
    23401 tgagtttgca atgcgttcca cggcctgccc tgagagcaca gcatgggaag cacctgcgta
    23461 aaccttcttt gcgccttttt caagcagcgc gtctactgtt tttatcagcg tgccggccgt
    23521 gtcaataatg tcgtcaataa tcaggcacgt ccttccctgg acgtcgccga ttacgtgcat
    23581 cacttccgtc acgttgatgt ccgtccggcg cttgtccaca atcgccagcg gaacacccat
    23641 ctttgtcgca aaatgccgcg cgcgttccac acctccggca tccggcgaaa ccactgtcaa
    23701 gtccggcaga ttcagttccc tgaagtagct caccatcacc ggacttgcga acaagtgatc
    23761 taccgggatg ttgaagaacc cctgaatctg cgccgcgtgc aaatccataa acagcgcacg
    23821 gtttgctcct gccgtcgtca gcaggtccgc gaccagcttt gacgaaatcg ccacgcgggg
    23881 acggtccttg cggtcctgcc tggcatagcc gtagtacgga acaacaacgg taatccttcc
    23941 ggccgaggcg cgcttcagcg cgtcaatcat aatcagcagc tcgaccaggt gctggtccac
    24001 cggatagcat gtcggctgga ccacaaatac atccactccg cgcacattct caaggagctg
    24061 aaagtagacc tctccatccg agaagcgctg catcttggtc tcgcccagcg tgaccccgat
    24121 gtgcctgcag atttccaggg caagctcgcg attggctgaa ccggaaaata tcttgaaccg
    24181 ccggtcttca ttcagcccgc gtgagcgctt gcgttctgcc gctggcttca ctcgcccgtc
    24241 ctgctgcgtt gtttctttcg ccgtttcggg cgaaatgacc tctaccgttg cttcgttctt
    24301 cacaagaacc tcccaagctg gtttgcgttg gttgtcctca tgctggccgg actgcacagt
    24361 gaccagcggc ttccgttgtc gcgctgcgat ggcccgtgag cgttggtcac gaaacaccgc
    24421 agcgaaaatc tggctgggcg accaggattc gaacctggac aaagtgctcc aaaggcactt
    24481 gacctaccat tagtcgatcg cccagtgaaa cagttgcaag ctgttagatt ccagtacccc
    24541 gttgttcgtt ttgactgggg accaacaact cataactact cattctcagc caacatcatc
    24601 ttccagtatg cctcccgcgg caaggtcttt gtccgcagga aagctgttcc gtgggatgac
    24661 agccgtttcc cggcttctgc tgctgcctct tccgtccggt acagcccaaa gatggccgaa
    24721 ccagaaccgg agagcgccgc atagacagcc ccaggttcca gaatgcgctt gatttggccg
    24781 agagagggat actgacggaa aacgacctct tcaaagtcgt tctgaatccc ggttcggaca
    24841 agcgcgagaa gcggatcctc gagctggcct tctcccgaag agaaggcgcc ggagaggcac
    24901 gctgaataga cgcggcctga ctctttgagt ctatcggatg ccgcccctgc ggtcaatctt
    24961 tgttcctgtg acgcatccca atcgcgaaag gcctgggggg tcgaaacacc aacggcaggc
    25021 aaagccacga cgcaatgcga ctcaggcaaa tccggcatgg gacagacctc ttcgccacgc
    25081 cccaaaccga ggaccgcgcc gccaaccaga aacagaggca cgtccgagcc tacttcctca
    25141 gcgatacgca gccgttccgc cccgggaagc cgcattccca gctcacgctc cagaccgatg
    25201 agcgcagcaa tcgcattggc cgatccggcc cccagcccac cctgtacggg aagccgtttt
    25261 tcgatgtgga catgcacctc cgcagaaaaa ttggccgcag caagcacgcg gtcgagcatc
    25321 ttccagaccg tgttcctcgc atccgtggga acacgctcat cgctggccgt aatggcgatg
    25381 cgggacggag tgggcgcttc gccttgggtg cccgatgcaa gatttctgct ggccagtgtg
    25441 gcttcgtcaa cacgaacggt caccagatca tgcgctgcca gcgtctgata cagcgtcgtc
    25501 agtgcatgaa aaccatcagg ccgcaaaggc ccgatggcca gccctaaatt gatcttgcaa
    25561 aaggatcgaa cacgcgtaga catgcaaaat ggattgtaac cggacagaca taaaaaggcc
    25621 cccggaatgg acgggggcct tttgcaggtg cagaggtgtt tatttcatgt tgccctggtt
    25681 gtccatgaca attccgccag gggtctggtt cttcttctgt tcgttgatct tgcgtgtttc
    25741 catggtcttc tgtgtccact ggacggcctg gtccacatct tccttgcggg cctggtcgtt
    25801 accgcattcc agattggcct tctggcggta catgaggttc atgtaggcca tggcgtcgtc
    25861 ataattgggc cggatggtaa gggccttttg caggtattcc agtccttcgc tcacgagagg
    25921 cccattctgc tctgccagct tctggcaggc ggccttcggc attttgggat tgccatcgcc
    25981 ctggtcctgc aggccgacgg cggaaagggc ctccacctgg tttttgtgcg caatgcccca
    26041 gtcaatcaca ccgatggtat aggccgcttc agcatcctgc gggtcaacgg caaggacctt
    26101 ctgttgccac tctttggctt tatcaaattc gtcaatctgg aagtagaggc tggcgatgcc
    26161 cttcagactg ctgacatctt tcgggtcttt cttcagtaca tcctggaaac cctcgatggc
    26221 caggttcgca ttcttcagat tttcaggcgt cttcaggtcg ggaacaacct gctgcgcata
    26281 ggccgttgcc agataaagtt tggccatggg gagcgaggga tcgaggttta ccgcattctg
    26341 gaaatggccg attgcctctt catacctcgc atttttgtag gcagcaacgc ccttgttcaa
    26401 ctggtcgcgc gccttgaggc ggttgcaccc ggtcaccatg gtcagcatcg ccaggaacgc
    26461 tgccgccatt gccggaacac gtgcgaatcg tttcattctg tttctcatct ccttatgaat
    26521 cgcctgattg cctgcaagcg gacagtacca gggacctcag aacaattatt tgctacgagc
    26581 atctgcggcc atcaccggca cgccctgcga ttgcgccgtc ccggaaggcc atgtccaaac
    26641 cgcgcacaaa ccgggagccg aaatctctct gcgctgtgat tgtaattggt ttccgccgaa
    26701 tggcaaacct tcaggcgaca gaccgaaagt tggcgcgaaa aagcgggcct actggcccgc
    26761 ttcaatcttc ggagtcacca ggccgatgtg atcaattccc agtccacggg ccatatccac
    26821 gacttccgct accgcctgat agttcaggtc agcatcaccc ttgatgaaga tgaccttctc
    26881 agcgcgaatc gaaaagatcg cgttgaggcg gttctgcagg tcgctctttg gtacgttgtc
    26941 ctggttgatc atgtacgtcg gctgggcgcc gccatgcgca atgatcgaaa caacaatgtt
    27001 cagatcgttc tgcttttgct gttgcgggtt cttcggcggc tgaggcacct gcgcctccag
    27061 gccgtgcggg gtgaccggca cgatcaccat gaagataatc agcagcacca gcagaacgtc
    27121 aatcaacggg gtaacgttaa tatcggcact cagtccaccc ggaccagaac cagttcccat
    27181 tgccataagt caaatcctcg tttctacgcg gtccgatagt caaaaaccgg ctattgtccg
    27241 ccaatctggt tcgtgcgctg ttcagtcagc aggccaacct gctccacgcc cgaagtacgc
    27301 actgcgtcaa tggcgtccat cacggtcccg taatgcgcgc gcgcgtcagc gcggaaataa
    27361 accatcttgt tgatcttgtt ttgcagcagg tcgccgactt tagaacccag gtcggagagg
    27421 gacacttcat tttgtcccag aaaaaccttg ctgtcacggg tcacagccac aacgactgcg
    27481 tcttcatggt ccgcgtcagg catggcggta gcattttccg tcctcaccat gtccacgttc
    27541 accttgttct gcagcattgg cgtgatgacc atgaagatga tcaacagcac cagcatcacg
    27601 tccaccatgg gcgtgacgtt gatgtttgaa ttcaccttct tgccttcatc tctcttaact
    27661 aatgccatct ttgctcccct gaatcgggcc ttctgatcaa tttcctgaca tgcgggcgga
    27721 ccctgcaaaa aaccgctgga ggcggaacgc tatgcgcgtc ccgctccaaa cgcaggattc
    27781 ccgcaatcga accgggccgc aatcagcggc ggttgctctg cttcaggaag taatcaatca
    27841 gttcgctcga ggagttgtcc atctcgacgt caaacgcttc caccttgttg gtgaagtagt
    27901 tgaacgtcat aacggccgga atggctacca gcagccccat agcggtggtc accagggcct
    27961 ctgaaatacc gccagccacg gcgccgatac cagacgtctt ctggactgcg atctgacgga
    28021 aggcgttcag aataccgacg accgttccaa acagtccgac gaacggggcc gtggcgccga
    28081 tggtggccag accgcccaga ccgcgcttca gcttggcgtg gacgatggct tcggcacgct
    28141 caagcgcgcg cttcgaggac tcgatctgct cctcgtttac tgcaccgccg ccaccaaagc
    28201 tgcggaactc ctgcaggccg gcagtgacca cttcggccag gtgcgacttc ttgttgcggt
    28261 cagcgacctt cacggcctca tccagcttgc cttccttgag cgctccggcg acacggggag
    28321 caaactcgcg cgactgcttg cgggccgccc cgaaataaag ccagcggtca ataatcacgg
    28381 ccagtgacca gatggactca atgaacagaa tgatcacgac ggtgcgcgcc agccagccca
    28441 tgttgcccca gagttcgagc gggctgaatc ctaccgtctg ctcctggaaa aaggccaggt
    28501 tggccgggtg aagaactgcg tgtgtaagtt gagcgagaat cactgaatct ttcctcctgc
    28561 ggagtttttc ttctttgctg ctgcctgcgc aattttccgc gcacggacaa aattacggtt
    28621 cgccccgttt gccgaaacgg gaccggacga aaaagcgtga cggttgcgga tcagccaccc
    28681 agagtaaaga ccacgttgac ctgggtttcc acctcgaccg gctcaccgtt gagcaggtaa
    28741 ggcttatagc gccaggtctt cacggcatct aaagctgcct gctgcaacat cggcgggcca
    28801 ctcacaacgt gcagattcgt aatggtcccc tgcttggaaa tcgtcgcctg caacacaacc
    28861 gtgccctgaa tgcgcgcggc cttggcaatc gcgggatact gcggctgggt cttcacgagc
    28921 aggttgccct gcatgacgcc cgacgacaca gcaatcttct tcggcggagc ggcctttaca
    28981 acaggcgccg ggccattgcc gattccacca agcacgccac caagcacgcc gccagaagag
    29041 ccgcccatgc cttccatgcc ggccacgcca gcggccggag gcggcgcttc ttcatgcacc
    29101 attttgattt ctttggggat cttggtgaac gtcatctggt tgttcaccag ctcggaaact
    29161 actttatgca caacaggcgc ctgcggcgga ggtggcggag gcggcggagg cggcgggggt
    29221 ggcgcaacca gcaaagtaga catcatcgcc ttcggcagcg cctcaggata aatcagcggg
    29281 tacagaatca tcccggcaag aatagaccca ttcagaacaa acccaatgat tgtgaagtac
    29341 ttactcctgg tcttgatctt gccgcctgat tccatcaagg catcttcaaa catggcctta
    29401 cctcttccaa gcgaaactcg ttaccaccta tagacaccat gcaggtgtct atttgttccc
    29461 gttgcaatca atttttctgg agcagatgga gcacttccga aaaagctgag tgagaccacc
    29521 tatatcacat cgcgcatacg tctggaaacg tttcaccaca tcaaactttt acttttcgcc
    29581 gagatgcacc atttccggtg ccaggtgtct ggggccccac cctttgcgca gcaaagaatg
    29641 ggaagcacac gcacctcaga cctttgcctt ctttgccgca ggcagtaccg ctgccttgcc
    29701 gcgccgctgg cgccaatcct ggtatgccac caacatcggc gcagccacag caatagatga
    29761 atacgtcccg atgagaatgc ccaccaccaa tgcaaatgaa aatccatgca acacttcgcc
    29821 gccaaacaga tacagcgaga gcactgtgag aaacgtcagc cccgaggtca ggaccgtccg
    29881 gctcagcgtc tggttgatgc tccggttgac caggtcggcc agaggctcgc gccgcgacat
    29941 ccgtaaattc tcccggatgc ggtcgaagac cacaatcgtg tcgttcatcg agtagccaac
    30001 cagcgtcagg atggcggcaa tcacagtcag cgagatttcc tggttcgtca gactgaatgc
    30061 gccgaccgta atcagcgtgt catgaaacac agcgacgaca gccgccacgc cgtaaatcag
    30121 ctcaaagcgg aaccacaggt agacaagcat ccccagcagc gaatagagaa tggcccacat
    30181 cgcctggttt cgcagttgcg cgcccacctg cgggcccaca atttcgacgt tgcgaatggc
    30241 aaatccggaa agatacgcat cctgcttcag cgcgcccaac accgcaggag agactttgcc
    30301 ggaaagctcg tctactgagc caatcagccc gctgtgctcg tcgcggtagc tcaggacggc
    30361 cgcagcctgc tggctatagt gctgctgcgc tgcatcattc tggcccaggt gctcggggtc
    30421 tttctgcgtc agccagttgg ccagcgtgcc ctgtccggca ttatccaggt ccagcttgcc
    30481 tgcattcgcc tgattgttgc gaacgtaatg attttccagt gcctgcagaa tcgccgcgcg
    30541 cccctggtcc agggcgttct cactggtatg gctcaacgcc agtgagatga tgacctcgtt
    30601 atccgccggc ctgccatacc gctggatgcg gacgtctttg attccggcgc tgtccgaggc
    30661 agaccgtatc cggtcaaggt caggcgtctg gtcgaacttc acccgcacca ccgtaccgcc
    30721 gcgaaaatcc acatccagag ggatatggtg ccagaaaagc atggagagca cgccagcgac
    30781 gctgaaaatc agcgaaaagc ccaggaaata ccacttcttc cctagccagt caatctttac
    30841 gtcatgaaac agttccacgg aatttcctct tattcagcta aaagcggtca agctaagttg
    30901 gcggctttat gctgaccgct ttatcgcttt ttaaatcgaa atcggctcac ccggacgctt
    30961 cttgttcagg tgcgcatcaa aaatgacacg cgagacgaac accgctgtga acaggttcgc
    31021 cagcagacca aaggtcagtg tcacggcaaa tccgcgcacc gggcctgtcc cgaagatgaa
    31081 cagaatcgcc gccgatacaa tcgtcgtcac gtgcgtgtct acaatggtcg tccaggcatg
    31141 ggcaaagccc tggtccacgg ccgccgatgc tgacttgccc gcgcgcagct cttcccggat
    31201 gcgctcaaaa atcagcacat tcgagtccac gcccatacca attgtcagaa tcacaccagc
    31261 tatgcccggc agcgtcagca cggagtgcgt aaagcccatg aagcccagca gaatcaccag
    31321 gttcaggaac agcgccaggt ctgcattgat gcccgcacca cggtaataga tcagcatgaa
    31381 gatcatcacc gccagcattc ccacgaccga ggccagcact ccttcgcgga tggaatctgc
    31441 gcccagcgac ggccccacaa cactctcgtt caggtaatgg attgaagccg gaagcgcgcc
    31501 ggtccgcagc agcatcgcca ggtcttttgc atactgctcg gtgataccgc cgccggagat
    31561 tgtcccctga tcgcggatgg tgtcccggat ggtcgccact tcgcggattt tattgtccag
    31621 caccacgcac agcggcttgc ctttgttggc cgttgtaaag tctgcaaaat ggtttcccgc
    31681 cgccgccgtc agataaaaga ccacctgcgg ctcgttcgtg ttcggattgc gccccggctg
    31741 ggcatcgcgg atgtccgtcc cggccacttc cggcacgcgc ttgatctcgt ataccgcctc
    31801 attgctgttc ggtcctgcgg ccccgggaat ggcgcgcatg agcacagaat cgtaaggaac
    31861 agagccgccc agcgcctgca atgccgtctg ctcgtccggc cacgggccgc cttccaccag
    31921 atgcagctca agccgcgccg tggactggat cacgtctcgc acgcgcgccg ggtcatctac
    31981 gccaggcagc tccaccagaa tctgatacgc gccgagattg tattcctgaa tcaccggctc
    32041 gcttacgccc agcgagtcca cgcgtgtcct gatgacctca atcgcctgct ccagcgcacg
    32101 cttcttcagg tcggcaacca ccgtcggctt catcgtgagc gtaaaggaat tgttctcgcc
    32161 cgacgaaatg tcgtactggc tgccatagcg ctggttcagc accgagcggg catcgcccag
    32221 gcggtctgcc ggcactccgg aaatcaggat ggtctccgga tgggcagaat ccggcttggt
    32281 aatcgaacca accgtgatgc cgttctgctg caaatcggtc tgcaggcgcg ccacatcgtt
    32341 gtccgtctgc gagccgacgg cctcgtccac catcacctgc agaatcaggt gggtaccacc
    32401 cctgaggtca aggcccagat ggatgcggtt gaggatggct tccttgagcg ctgtaccgct
    32461 caggcccctc gggataccga aaatcccaaa cagaaagacc agcaggatcg caataatcag
    32521 gatcgttttt tgagtcaggt tcttacgcat ggatatctac ttcgcaggct cgtcggtggt
    32581 cacagaggcg atcgcgctct ttacgacctc catcttaatg ttgtccggag gcacgcggag
    32641 ctggatggcg tcgtccttaa tcgaaaggat ggtgccgcga atgccgcctg aggttgtcac
    32701 ccggtcgccc ggcttcagat tgc
//
LOCUS       DASRBV010000019         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_456699, whole genome shotgun sequence.
ACCESSION   DASRBV010000019 DASRBV010000000
VERSION     DASRBV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456699"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFA02_00830"
     CDS             complement(<1..360)
                     /locus_tag="VFA02_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08418.1"
                     /translation="MLQRRSFPVFLCASILMLLCGLRAAHADAALLLEEPFGEFGNMN
                     PTGHAAIYLDHVCAASLTQLRICQPGELGVVISRYHKIGGYDWLAVPLIPYLYAVDDP
                     AEVPESITPQKELLLRDR"
     gene            585..1298
                     /locus_tag="VFA02_00835"
     CDS             585..1298
                     /locus_tag="VFA02_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU08419.1"
                     /translation="MAYSSTITPGQTLLLDADDTLWENNIYFERAIADFISYLNHQSY
                     DREEVRAFLNEVERETVRERGYGLASFQHSLIVCFERLSNSPITPEKHERIVGFARSI
                     SRYEIQLLPGVAEVLPALASRHQLILMTKGNEAEQKDKLARSGLAHHFAAVEVPKEKD
                     PDAYHFVRQKYGLDHRSTWMVGNSPKSDINPALAAGLHAVFIPHPHTWMLEHEAVGSA
                     QNGQQLLQLDSFREMGLRF"
     gene            complement(1295..1654)
                     /locus_tag="VFA02_00840"
     CDS             complement(1295..1654)
                     /locus_tag="VFA02_00840"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HZU08420.1"
                     /translation="MAKKKTAASVASQVTSVDDPNVILFQGPKPLAKPGTVQLHSFGT
                     VIDKIFGRKVEDVKADFDKVSDQLNTILSTAFSKVTAGMKMDEVEIALGFTAEGKLAF
                     IAQAGVEASVKVTFKRA"
     gene            complement(1673..2716)
                     /locus_tag="VFA02_00845"
     CDS             complement(1673..2716)
                     /locus_tag="VFA02_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08421.1"
                     /translation="MPFMQEAAMGFEFEMDARRNEPRAQEQEYSEWVTKESFANTSMN
                     WSSGPKLLPESWPSEEALGPVHRVLQTNPALFDGFSEDQARTVFWTIAIQNAFELGGH
                     AKEIPGFRMASYGFFDPETELDGFGVIAYVNPSLHGPAKGEFQWVTVDGQSFPVIARA
                     AVWKLHAPSVHPSLGTSTCWANSRRPTLKNKPAIVTAKHVVGTAIPIGSAIPMTSGHG
                     TLLDLAPEGIDAALVQISRTHWPRNPTQLSCQKFVAQWTDVNVYSPTGVISTKVTEVS
                     SGRGTLDPSIPLRIFLANPGQSGDSGALIIDSTGKGIGLYMGEVTTPANLQEGFCQHL
                     GQVEEAMSVDLFL"
     gene            2918..>3451
                     /locus_tag="VFA02_00850"
     CDS             2918..>3451
                     /locus_tag="VFA02_00850"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU08422.1"
                     /translation="MDTCKQFVEPPVRGFLHQPADPGGDSLILTHGAGADCNAPLVVS
                     LAEAFCASGITVLRCDLPFRQSRPHGPPARGSAEHDQQGLRAAVAAMRKQTSGRIFLG
                     GHSYGGRQASMLAATGPGLVDSLLLLSYPLHPPRKPDELRTGHFPSLRTPALFVHGTR
                     DVFGSIAELANALRLIPA"
BASE COUNT          772 a          915 c          925 g          839 t
ORIGIN      
        1 gcggtcgcgt agcagcagct ctttctgcgg agtaatgctt tccggaactt cagccggatc
       61 atccacggca tatagatatg gaatcagagg gacagcgagc cagtcgtatc cgccaatttt
      121 atgatagcgg ctgatgacga ctcccaattc gcctggctgg cagatgcgta gctgcgtcag
      181 cgatgcagcg cagacgtggt caagataaat agctgcgtgt ccggtgggat tcatgttgcc
      241 aaattcgcca aacggctctt ccagcagcag agcagcatca gcatgggcag cacgaagacc
      301 acaaaggagc atgagaatcg aggcgcataa gaatacagga aagctgcgtc tctgaagcat
      361 gaaaaagtct ctccttatac agataggatg tttaaactgt tctgtattct tgctgacggc
      421 ttttcgccgt gagtggacta ataaagtaga cccggatttc taaaaaaaga tggcgatatc
      481 ttcaacaaaa aaaatgtctt tggaaaggca accccatcgt tgcattttag atgccgtgtg
      541 atgatgcagt gcgcgccggg tatgtacttt gttactctga aagagtggcc tactcttcga
      601 cgattactcc agggcagact ctattgctgg atgccgatga cacgctctgg gaaaacaata
      661 tctattttga gcgtgccatc gctgatttta tctcttatct gaaccaccag agttatgacc
      721 gcgaggaagt ccgcgctttt ctgaatgagg tagagcgtga gaccgtccgc gaacgcggtt
      781 atgggctggc cagttttcag cattcgctga ttgtctgctt cgagcgcctg agcaattcgc
      841 ccattacccc tgaaaagcac gagcgcattg tggggttcgc gcgctccatt agccggtatg
      901 aaattcagct tttgccggga gtggctgagg tgcttcctgc gcttgcatcc aggcaccagc
      961 tcattcttat gacgaaaggc aatgaggcgg agcagaaaga taagctggcg cgttctggcc
     1021 tggcgcacca ttttgcagcc gtagaagtac cgaaggaaaa agacccggat gcgtatcatt
     1081 tcgtgcgcca aaaatatggg ctggaccatc gctcgacatg gatggtcggc aacagcccaa
     1141 agtcagacat caatcccgcg ctcgcagcag gattgcacgc ggtatttatt ccgcatcccc
     1201 acacctggat gctggaacac gaagctgtag ggtcggcgca gaacggccag caattacttc
     1261 agcttgatag cttcagagag atggggcttc gattttaagc gcgcttaaag gtcaccttta
     1321 cgctggcttc cactcccgcc tgtgcaataa aggccagctt gccttcggca gtaaatccaa
     1381 gtgcaatctc tacctcatcc attttcatcc ctgctgttac ctttgagaat gcggtagaca
     1441 aaatcgtgtt cagttggtct gacactttat cgaaatcagc ttttacatct tccactttgc
     1501 ggccaaagat tttgtctatg acagttccga aggaatgcag ttgcaccgtg ccgggttttg
     1561 ccaaaggctt cgggccctgg aacaaaatta cattggggtc gtccacgctg gttacttgag
     1621 atgccacgga agctgcggtt ttcttctttg ccatgttttc acatcctttg ctttaaagaa
     1681 acaaatctac tgacatcgct tcttctacct gtccgaggtg ctgacagaag ccctcctgca
     1741 agttggcggg cgttgtcacc tcgcccatgt agagaccgat gcccttacca gtgctgtcaa
     1801 tgatcagtgc gcccgaatca ccgctttgtc ctgggtttgc cagaaagata cgcaatggga
     1861 tactcgggtc cagagtgcct ctgccgctgc tcacttctgt aactttggtt gagatgacac
     1921 ccgtcgggct atatacgttt acatctgtcc actgtgcaac gaatttctgg caggaaagtt
     1981 gggttggatt tctgggccag tgcgtcctgg aaatctggac aagtgccgca tctatgccct
     2041 ccggagcaag gtcaagcagc gtgccgtggc cgctggtcat tgggatagcg ctgccaatgg
     2101 gaattgcggt cccgaccaca tgcttggccg tgacaatggc aggcttgttc ttcagcgtag
     2161 ggcggcggga atttgcccag caggttgaag tacccagcga tggatggact gaaggagcgt
     2221 gtaatttcca caccgctgcc cgcgctatga ctgggaaact ctggccgtct actgtcaccc
     2281 actgaaattc cccctttgcc ggtccgtgca gtgacggatt tacataggca atcaccccaa
     2341 agccgtccaa ctccgtttca ggatcgaaaa agccatagct ggccatcctg aaccctggaa
     2401 tctcttttgc atgtccgcca agttcaaagg cgttctggat ggcaatggtc cagaacacag
     2461 tgcgggcctg gtcttcggag aatccatcga agagcgccgg attcgtctga aggacacgat
     2521 ggactgggcc tagggcctct tctgatggcc agctttcagg cagcagtttt gggccactgc
     2581 tccagttcat gctggtgtta gcaaatgact cttttgtgac ccattcagaa tattcctgct
     2641 cttgggcgcg gggttcattg cgacgagcat ccatttcaaa ttcaaagccc atggcggcct
     2701 cttgcatgaa aggcattttg gtgggggaac aatacgccta agatgagtgc tgcaactgta
     2761 cgacggagac ggtttctcgt caatcctgga atggccgaag gctggatttc tgtgcacctc
     2821 gaccacctgc cagcccagaa agaatgccgg agcagacccg cagcgctgtc catctgcggg
     2881 aatcaacgag ataatcagta gcgggttgtg acgtctcatg gatacctgta aacaatttgt
     2941 tgaaccgcct gtacggggat tcctgcacca gcctgctgac ccaggaggag attccctcat
     3001 cctgacgcac ggagcaggag cagattgcaa tgctcccttg gtggtttctc tcgccgaggc
     3061 attctgcgct tcgggtatca ccgttctgcg gtgcgatctg cctttccgcc agtctcgacc
     3121 gcatggcccg ccagcgcggg gaagcgccga acatgaccag caaggtctgc gtgccgcggt
     3181 tgcagccatg cgaaaacaga cttctggtcg gatatttctg ggcggtcact catatggagg
     3241 cagacaggca tccatgctcg ctgcaaccgg gcccggtctt gtggactctc ttctgttgct
     3301 ttcctatccg ctgcatccgc cacgaaagcc ggacgagttg cgaacaggac attttccatc
     3361 tctccggaca cctgcgcttt ttgtccatgg gacgcgagac gtctttggct ccattgctga
     3421 attggcaaac gctctcaggc tcataccggc c
//
LOCUS       DASRBV010000020         7727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_609075, whole genome shotgun sequence.
ACCESSION   DASRBV010000020 DASRBV010000000
VERSION     DASRBV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609075"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..892)
                     /locus_tag="VFA02_00855"
     CDS             complement(161..892)
                     /locus_tag="VFA02_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZU08423.1"
                     /translation="MSEVLPKLALLAGGLATRLGPLTRSVPKSLVRICGVPFLAYQLE
                     LFAGQGICEIVICAGHLGRQIEAFAGDGSRFGCSIAYSHDGDMLLGTGGALRRALPFL
                     GECFFVVYGDSYLTADFAEVWRAFLVSGKPALMTVFRNEGKWDTSNVEMRDGTIVRYS
                     KLARTAEMQHIDYGLSILRAEVLRHWPDGAQFDLAGVLSSIAEDGQLAAYEVNERFYE
                     IGSPEGLRMTEEHLAGTMVLAGGPQ"
     gene            complement(885..1886)
                     /locus_tag="VFA02_00860"
     CDS             complement(885..1886)
                     /locus_tag="VFA02_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZU08424.1"
                     /translation="MPVFSSAQYSGRGRRYFLTGGAGFIGSHFTDVLLGNSSVAAVTV
                     YDNLSSGREWHIQQHLENPQFQFIRGDISDLGFLTHVMAGHDVVIHLASNPDIARAVN
                     DPEIDFRQGTALTNNVLEAMRRSGVPRLLYASGSGVYGDLGEKALREDHGPLLPVSTY
                     GASKLAGEAMISAYCSMFGMTACVFRFANVVGARQTHGVGFDFVRRLMENPRRLRILG
                     DGNQSKPYIHVSDVVSAVLCAEERCSDAFSVFNVATDDAITVTEIACLASECLQIVPQ
                     PEFDYTGGDRGWKGDVPVVRLNADKISSLGWRPHANSYEAMRRALMEMIADERECYV"
     gene            2179..3153
                     /locus_tag="VFA02_00865"
     CDS             2179..3153
                     /locus_tag="VFA02_00865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU08425.1"
                     /translation="MQRCSVTVGLPVCNAMPFLPEAVESLLSQTETNFRILAIVDGGG
                     DGSLEYLLSVRDSRLRVISQAHSGLSFTLNRMLRETETPWLVRQDADDISHPARIERL
                     LEAIAKYPEAGMFYSLAEYHPRKRSVGLFRCSRGSPQQLRQIVQRGYLLSICHPSVAL
                     NVRKTLAIDGYRDLRHAEDTDLWWRMALHYDVHLIPEVLVGFRHNAGSVSSQHSYTQE
                     LHGLYIQYLLLSHLLGKTPQPLNVIAPLLQTSISDRRFAAKQHLRNLNMHLAARRYLR
                     ASASLARSFLSSPGYLLGRLKDELLPNGTIVNGIPPEMFYRRKEVFWP"
     gene            3156..3950
                     /locus_tag="VFA02_00870"
     CDS             3156..3950
                     /locus_tag="VFA02_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZU08426.1"
                     /translation="MHTRCNIAGVPVDNTTLESAATGIVEAVRKRALSRPLLIMGPNA
                     QLVVLAQRNARFAKALRSADLNVPDGISIVLASRLLGSPISGRVTGGDLMERLCAEAA
                     LHGLSIFLLGGLPTAARLAARRLERKYPKLKVAGTYCPPLGFEHDPEENARIRRLITE
                     ARPSLLFVAFGAPKQEIWIHENCPALPIGAAMSVGAAFDTQAGLRRRAPGWTHKIGME
                     WLYRLLREPRRLWRRYLFGNAYFIYCVLKQKLHSKDLHQIQPEFHP"
     gene            3950..4699
                     /locus_tag="VFA02_00875"
     CDS             3950..4699
                     /locus_tag="VFA02_00875"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZU08427.1"
                     /translation="MMTQLEHLGIKLFCDAADFSSLLAWSADPRIQGFTTNPTLMRKA
                     RVLDYEAFARDLVRVVPDRPISFEVLADDPGTIEEQALAIARWGKNIYVKVPVTNTWG
                     AFMGPVLRSLSKAGVQLNVTALLTLRQVEQVAACLDPSTPAIISVFAGRIADTGIDPV
                     PIMRQAARITAAASNIELLWASPRELLNIFQAEEAGCDIITLLPEILARLDLVGKDLE
                     EYSLETVKMFYKDACAAAYQIDTRKPIALTA"
     gene            complement(4660..5241)
                     /locus_tag="VFA02_00880"
     CDS             complement(4660..5241)
                     /locus_tag="VFA02_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU08428.1"
                     /translation="MKRAVFLDRDGTLNRNIWNPVTGAYESPLYPEQMELVPGAASAL
                     KLLRDAGFLLVLVSNQPNAAKGKATARALDAIHARFECLLREEGIVLDAVYYCFHHPE
                     FTGPCICRKPSPYFLFHMRDKSGVNLAESWMIGDRITDVQCGLAAGVKTIFLSQSNPA
                     ETSAGLAAPDIWVAAQMVVAHHAVRAMGFRVSI"
     gene            complement(5238..5846)
                     /locus_tag="VFA02_00885"
     CDS             complement(5238..5846)
                     /locus_tag="VFA02_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZU08429.1"
                     /translation="MTGYTERFLDEVRAITRLLNIEKIEEMVFHLAEVRENGGRVFFL
                     GVGGGAGHASHAVNAFRKIAGIECYAPSDNVSELTARINDDGWESCYANWLRGSRING
                     QDAVFVFSVGGGSRVRNISMNLVHSLELAQRVGARILGVVGRDGGCTAQVADTCIVIP
                     TISEDTVTPLVEAFQAVVWHLIVSHPRLKANEMKWESEAVLV"
     gene            complement(5815..6780)
                     /locus_tag="VFA02_00890"
     CDS             complement(5815..6780)
                     /locus_tag="VFA02_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU08430.1"
                     /translation="MPARPLVSAVIPTLERPQWVVRAVKSALAQSYAPVEVIVVIDGP
                     DEETESALKKITDVRLRVIPLAKHAGGAESRNIGVRAASGEWIAFLDDDDEWLVQKLE
                     RQMQAALQSGAKYPVVSSRLMAGCVLPRRLYRQGHDMSEYLFCRKGPAYGDGMMQTST
                     LLAKRELLLEVPLQKGLKRHQDWDWLLKIAQHKDVEIAMLPDALTIMRVGSCGPGVSN
                     SGDWRVSLEWARANRLRISARAYSFFITTECVTRARKAGAGIPALTELLWECLTRGRP
                     GFRQMMLFVLFSVLPGEKRRTLRDRFYRRAGKRFHNDGLHRAFSG"
     gene            complement(6881..7600)
                     /locus_tag="VFA02_00895"
     CDS             complement(6881..7600)
                     /locus_tag="VFA02_00895"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZU08431.1"
                     /translation="MGTPSIRVQLECALKAAGNPERAAFMARYFKTGKGEYGEGDVFL
                     GIAVPVLRKIALAHRDIDFADLQRLLDSKIHEYRAAALEILVSQYNGGDGKLRREIVA
                     FYLKNTARINHWDLVDTSCRPILGGYLKTRPRKILRRLAKSKSLWERRIAMVSTMALV
                     WDGELDDAIEIAEMLLDDDHDLIRKAVGWVLREVGVQDRAVLLAFIKKHYERIPRIAL
                     RYAIEHFTSAERKKVLAGKFT"
BASE COUNT         1745 a         2398 c         1910 g         1674 t
ORIGIN      
        1 tatttcagaa aatatcccgg caggaagctc ttgttaatgg tgatgtagac atatttattg
       61 attgccgccg aaatcacgaa tccgccaaat tcgcggtaat acgagggaag gtccgtgcct
      121 ccgccaccaa tcgaaatgcg cagaggagtg cgcgtaataa tcattgcggt cctccggcca
      181 gcaccatcgt tccggcaaga tgttcttcag tcatgcgcag cccttcagga gaaccaatct
      241 cgtagaaacg ctcgttgact tcataggcgg caagctgtcc gtcttccgca atactgctta
      301 acactcctgc aaggtcaaat tgcgcgccat ccggccagtg ccggagcacc tccgcacgca
      361 gaatgctcaa cccgtaatca atgtgctgca tctccgctgt gcgtgccagc ttgctatagc
      421 ggacgatggt cccatcgcgc atctcaacat tgcttgtgtc ccatttgcct tcattgcgaa
      481 agacagtcat cagcgcgggt ttgccagaca caagaaacgc acgccacacc tcagcaaaat
      541 cagctgtcag ataactgtcg ccatacacaa cgaaaaaaca ctcaccgaga aacggcagcg
      601 cacgacgcag cgcccctcct gttcccagca acatgtcccc atcatgcgaa taggcgatgc
      661 tgcatccgaa gcgcgaacca tctccggcaa aggcttcgat ctgcctgccg agatgcccgg
      721 cgcaaatcac gatctcgcaa attccctgcc ctgcaaacaa ctccaactga tacgccagaa
      781 acggcactcc gcagatcctt accagggact ttggcacaga ccgcgtcaac ggccccagtc
      841 gcgtcgccag tccgcccgca agcagggcga gttttggcag gacctcagac atagcactct
      901 ctttcgtccg caatcatttc catcaacgca cggcgcatcg cctcatagct gtttgcgtgt
      961 ggcctccagc ccagactgga aattttgtct gcattcaacc gcaccacagg aacatcgccc
     1021 ttccagccgc gatcgcctcc cgtgtaatcg aactccggtt gaggcacgat ttgcaggcac
     1081 tcagaagcaa gacaggcgat ttccgtaaca gtaatcgcgt catctgtggc tacattgaaa
     1141 acgctgaacg catcagagca gcgttcctcc gcgcacaaca cggcactcac cacatcagaa
     1201 acatgaatgt agggcttgct ctggtttccg tctcccagga tgcgcagccg gcgaggattt
     1261 tccattaggc gccgcacaaa atcaaacccg acgccatgcg tctgccgcgc ccccactacg
     1321 ttggcaaaac gaaagacgca cgccgtcatt ccgaacatcg agcagtaagc cgagatcatc
     1381 gcctcgccag ccagcttgct tgcgccatat gtcgagacag gcagcagagg cccatggtct
     1441 tcacgaagcg ctttttcgcc cagatcgcca tatacaccgc tgcccgaggc gtagagcaga
     1501 cgtggaacgc cgctgcggcg catcgcttcc agcacgttgt tcgtaagcgc tgtgccttgt
     1561 cgaaaatcaa tttccggatc attcaccgcg cgcgcaatat cggggtttga tgccaaatgg
     1621 atcacgacat cgtggccagc catgacatgg gtgagaaagc cgaggtcgga aatgtctccg
     1681 cgtataaatt gaaactgcgg attttcaagg tgctgctgga tatgccattc ccggcctgag
     1741 gagagattgt cgtagaccgt taccgctgcc acgctgctgt tcccgagcag aacatccgtg
     1801 aagtgactgc caataaagcc tgcgcctcca gtcaggaagt atcttcgtcc gcgaccgcta
     1861 tattgagcgg aactaaatac aggcatcaaa gacacagctc aactcccgta tggcctccct
     1921 tctgaaaaga agcacaagct attcagcttt gcatgattgg taagagcaac ttagcagaga
     1981 taggaagaga tacaaacatt acctaagttt gctgactact gcacagcaat cttgacgcac
     2041 gatacaggcg gcgcgatata catcatctaa ttaacaaaac aaactttcgt tgtactgatt
     2101 gccttatctg accttggccc agtctgtgtt agcaaaaaca ggcacgcctg cgcgcagaaa
     2161 ggcattgagg agtggcccgt gcagcgttgc tctgttaccg ttggtctgcc tgtctgcaat
     2221 gcgatgcctt ttctgccgga ggcggtcgaa agcctgcttt cgcaaacaga aacaaacttc
     2281 cgcatcctcg ccattgtgga tggcggcggc gatggaagcc ttgaatatct tctgtctgtc
     2341 cgtgactcgc gcctgcgcgt catttcgcag gcccattccg gcctgtcatt cacgctgaac
     2401 cgcatgttgc gggaaactga gactccgtgg ctggtccgtc aggatgcaga cgacatctcg
     2461 cacccggcgc gcattgaacg cctgctggaa gccattgcca aatatcctga agcaggcatg
     2521 ttctactcgc ttgccgagta tcatccgcga aaacgcagcg ttggactctt ccgctgctcg
     2581 cgcggttcgc cgcagcagct caggcagatc gtgcaacgcg ggtatctgct ttctatctgc
     2641 catccatctg tggccctgaa cgtccggaag acgctcgcca ttgatggata ccgcgatctg
     2701 cgccatgcgg aagatactga cctatggtgg cgcatggccc tgcattatga cgtccacctg
     2761 ataccggaag tgctggttgg ttttcgccac aatgcaggaa gcgtcagttc gcaacacagc
     2821 tatacgcagg aactgcacgg cttgtatatc cagtacctgc ttctctcgca cctgctgggc
     2881 aaaacacccc aaccactcaa cgtcattgct ccgctgttgc agacatccat ctcagaccgg
     2941 cgttttgctg caaaacagca ccttcgcaat ctcaacatgc acctagctgc gcggcggtat
     3001 ttgagagcat ctgcaagcct tgcgcgttcc tttctttcat ctccgggata tctcctcgga
     3061 cgcctcaaag atgagcttct gccgaatggc accatcgtca acggtattcc accggagatg
     3121 ttctaccgca gaaaggaagt gttctggcca tgaccatgca cacacgctgt aatatcgccg
     3181 gtgttccggt agacaacacc acgcttgaaa gtgcggccac aggcattgtg gaagctgtcc
     3241 gtaaacgcgc attatcgcgg ccgttattga ttatgggccc gaacgcgcag cttgtggtcc
     3301 tagcccagcg caacgcgcgt tttgcgaagg cgttacgctc tgctgatctg aatgttcctg
     3361 acggaatttc catcgtgctt gcctctcgcc tgctcggcag tcctatatca ggacgcgtga
     3421 ccggaggcga cctcatggag cgcctgtgtg ccgaagctgc tctgcacggc ctcagcatct
     3481 ttctgcttgg agggctgcct acggccgccc gactcgcagc ccgcaggctg gagcgaaagt
     3541 atccaaagct aaaggttgct ggaacgtact gcccaccatt ggggtttgag cacgatcccg
     3601 aagagaatgc acggattcgc cgcctgatta ccgaagctcg ccccagcctg ctctttgtcg
     3661 cattcggcgc tccgaagcag gaaatctgga tacacgagaa ctgtccggcc ctgcccatcg
     3721 gcgcagcgat gtctgtcggc gctgccttcg atacacaggc aggactgcgc aggcgcgctc
     3781 ccggatggac gcacaaaatc ggaatggagt ggctctaccg cctcttgcgt gaaccgcgcc
     3841 gcctgtggcg cagatactta ttcggcaacg catatttcat ctattgcgtt ctgaagcaga
     3901 agctgcattc caaggacctt caccagattc aaccggagtt tcacccataa tgatgaccca
     3961 actcgaacat cttggcatta agcttttctg tgatgctgct gatttcagca gccttctcgc
     4021 atggtccgcc gacccgcgca ttcagggctt caccaccaac ccgacactta tgcgcaaagc
     4081 gcgtgtcctc gactacgagg ccttcgcccg cgacctggtc cgtgtggttc ctgaccgccc
     4141 catttctttt gaggtgctgg ctgatgaccc agggacgatt gaagaacagg cgctcgcaat
     4201 tgcgcgatgg ggtaaaaaca tatatgtaaa agtcccggtc acaaatacat ggggtgcgtt
     4261 catggggccg gtcttgcgca gtctatcaaa agccggagtg cagttgaatg tcaccgcgct
     4321 gctgactctc cgtcaggtag aacaggtggc ggcgtgtctc gacccgtcca ctccggccat
     4381 catctcggtt ttcgccggca ggatcgccga tacaggcata gatcccgtgc ccatcatgcg
     4441 acaggcagca cgcatcaccg ctgcagcgtc aaatatcgag cttctgtggg ccagcccacg
     4501 cgagctgctc aacatctttc aggcagaaga agcaggttgc gatatcatca cgctgctccc
     4561 ggaaatcctc gcacggctgg acctcgtcgg gaaagacctt gaagaatatt cgctggaaac
     4621 agtaaagatg ttttacaaag acgcctgcgc ggcagcctat cagattgaca cccggaaacc
     4681 catcgccctg actgcatgat gcgcaacgac catctgcgcc gcgacccata tatcaggtgc
     4741 ggcaagaccg gcactggtct ccgctggatt gctctgcgag agaaaaattg tcttcactcc
     4801 ggcagcaagg ccgcactgaa catccgtgat gcggtcgcca atcatccacg actcggcaag
     4861 attaacgccg gacttgtctc gcatgtgaaa gagaaaatat ggcgatggct tacggcatat
     4921 acatggcccg gtaaattcag gatggtggaa gcagtaatat actgcatcca gaacgatgcc
     4981 ttcctcacgc aacaggcatt caaatcttgc gtggatcgca tccagcgccc gcgccgttgc
     5041 ttttcccttc gccgcattcg gttgattgga aacaaggacc agcagaaacc cggcatcgcg
     5101 cagtagcttc agcgcagatg cggccccggg gaccaactcc atctgctccg gatacagcgg
     5161 cgattcatac gcgcctgtga cagggttcca gatattacga ttcagcgtgc cgtcgcggtc
     5221 aagaaaaacg gcgcgcttca caccagcacc gcctccgatt cccacttcat ctcgttcgcc
     5281 ttcaggcgcg ggtgagaaac gatcagatgc cataccacag cctggaacgc ctctaccagc
     5341 ggcgtcaccg tgtcctcaga aatagtggga atcacaatgc acgtatcagc aacttgcgcc
     5401 gtgcatccgc cgtcgcgccc aaccacgcca agaatgcgcg cgcccacgcg ctgcgccagt
     5461 tccaggctgt gcacaaggtt catgctaatg ttcctcaccc ggcttcctcc gcctactgaa
     5521 aacacgaata ccgcatcctg accgttaatt ctgctgccac gcagccagtt ggcatagcag
     5581 ctctcccaac cgtcatcatt gatgcgcgcc gtcagctcgg acacattatc actgggcgca
     5641 tagcattcaa ttccggcaat cttgcggaag gcgttcacag catggcttgc gtgccccgcc
     5701 cctcctccca ctcccagaaa gaacacacga ccgccgtttt cgcgcacttc tgccagatga
     5761 aaaaccatct cttctatctt ctctatgttc agaagtcgcg taatggcccg cacttcatcc
     5821 agaaaacgct ctgtgtagcc cgtcattatg aaacctcttc cctgcccgtc tatagaatct
     5881 atctcgcaac gtcctgcgct tctctcccgg aaggacgctg aagagcacaa acaacatcat
     5941 ctggcgaaac cccgggcgtc cccgagtgag gcactcccag agcaattccg tgagagcagg
     6001 aattcctgcg cctgctttcc gcgcgcgcgt cacacattct gtcgtaatga aaaatgaata
     6061 tgctctggcg cttatgcgta gacggtttgc acgcgcccac tccagagaca cgcgccagtc
     6121 ccccgaattg ctgacgcccg gcccgcagct tcccacgcgc atgatggtca gcgcatccgg
     6181 cagcatcgca atctcgacat ctttgtgctg cgcgattttc agcagccagt cccagtcctg
     6241 gtgccgcttc agaccttttt gcagcggtac ttccagcagc agttcacgtt tggccaagag
     6301 tgtcgaggtc tgcatcatgc catcgccata tgcaggtcct ttgcggcaaa aaagatattc
     6361 gctcatatcg tgcccctggc gatacagcct gcgcggcagg acgcatccgg ccatcagccg
     6421 cgaacttacg accggatact tcgccccgga ttgaagggcg gcttgcatct gcctttccag
     6481 cttttgcaca agccactcat catcatcatc cagaaaagct atccattcgc cgctcgccgc
     6541 gcgcacgcct atattgcgcg actccgctcc acctgcatgt ttcgccagtg ggatgacccg
     6601 caatcgcaca tcagtgattt tctttaaagc gctttccgtc tcctcgtccg gcccatctat
     6661 caccacaatc acttccaccg gagcatagct ctgcgccagc gcgcttttca ccgcgcgcac
     6721 tacccattgt ggtctttcca acgtgggaat gaccgcgctt accagcggac gtgcaggcaa
     6781 tggctttcct taattggtga gatttgctca aagcatacaa aagctgcggg tgctctgaaa
     6841 gacttccgtg acaagcatct caaaccaaag taatgaagga ttacgtaaac ttacccgcaa
     6901 gcaccttctt ccgctctgca gacgtaaagt gctcaatcgc ataacgcagc gcgatccgcg
     6961 gaatgcgctc gtagtgcttt ttgatgaaag ccaagagcac ggcccgatcc tggaccccaa
     7021 cttcgcgcag tacccatcca acggctttgc gtatcagatc gtgatcatca tcgagaagca
     7081 tctctgctat ttcgatagca tcatccagtt cgccgtccca gaccagcgcc atcgtcgaaa
     7141 ccatcgcgat gcgccgttcc cacaggctct tcgatttcgc cagcctgcgc aagattttcc
     7201 gtggacgcgt tttcaggtac ccgccaagaa tcggcctgca tgatgtgtcc accaggtccc
     7261 aatgattgat gcgggctgtg ttcttcaggt agaaggcgac gatttctctc cgcagcttgc
     7321 cgtcgccgcc gttatattgc gacaccaaga tctcaagagc ggcagcacgg tactcgtgga
     7381 tttttgaatc cagcagccgt tgcagatcgg caaaatcaat atcgcggtgt gccagcgcga
     7441 ttttccgcaa caccggaacg gcaatcccca ggaacacatc accctcgccg tactcgcctt
     7501 tgccggtctt gaaatagcgc gccataaatg cggcccgctc aggattgccc gccgccttca
     7561 aggcacattc cagttgtacc cgtatagatg gggttcccat gcttttcttc tattgcgcag
     7621 caacctgcca aagcagctat tatgtcctac attcatgttg aatgcagccc aggcaattct
     7681 gaacaaaatt ttgtggccgg agcagccttc ttccgccagc tccagcc
//
LOCUS       DASRBV010000021         8908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_203942, whole genome shotgun sequence.
ACCESSION   DASRBV010000021 DASRBV010000000
VERSION     DASRBV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203942"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFA02_00900"
     CDS             <1..228
                     /locus_tag="VFA02_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08432.1"
                     /translation="LRTLYPYVQLVQSHIFGFSGEIAGTLSQAMMYLQFPLYGLLMSL
                     LLRARGFMTALLTTAIVHFGAVVIAVALAHL"
     gene            244..1116
                     /locus_tag="VFA02_00905"
     CDS             244..1116
                     /locus_tag="VFA02_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZU08433.1"
                     /translation="MADAVKIIECPRDAWQGLPKTIPAEVKAGYLRALIAAGFRHLDA
                     VSFVSPKAVPQMADSEQVLEYLDPPDDVEIIGIVVNQKGAERAIATEAVRTLGFPYSI
                     SSEFLKRNQGQTPEESLDVLETIGQSAYKAGLNLVAYISMAFGNPYNDAWSIDEVIAA
                     CDLLADMGIEQISLADTVGLATPDEVSLLVSAVLSANDGLEIGVHLHARPEEVAAKVA
                     AAYRAGCRRFDMAIGGLGGCPFAQDALVGNISSELAIAELERLGAELPSFQPLNSILA
                     ANRAIAEKFGPAKQ"
     gene            complement(1144..1914)
                     /locus_tag="VFA02_00910"
     CDS             complement(1144..1914)
                     /locus_tag="VFA02_00910"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZU08434.1"
                     /translation="MEPLVIAGKAFQSRLIVGTGKYKDGPETQAAIEASGAEMVTVAV
                     RRVNLDRSKESLLDYIDPQRYFLLPNTAGCYTAEEAIRAARLAREVGISDWVKIEVIG
                     DEATLYPDVQATLEATRILVKEGFTVLPYTSDDIVFAKRLIDAGASAVMPLAAPIGSG
                     LGIQNRATLQILREMVTEVPLIVDAGVGTASDAALAMEMGADGVLMNTAIAAAANPVL
                     MAEAMQHAVLAGRQAYLAGRMPRKLYATASSPLEGISK"
     gene            complement(1940..3952)
                     /locus_tag="VFA02_00915"
     CDS             complement(1940..3952)
                     /locus_tag="VFA02_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZU08435.1"
                     /translation="MTLLKCVVAGVVSFSFCGFAVAQRLNRADLSTVNAGPSTPALHA
                     YAQDGNLPVTARNNHSFTQAQSCAEEQTSGPCVDKIEPPNWWIHMPSPMLLLHGNHLN
                     GAHFLVRGRNVGLTKTQISQNGHWAFLWLDTTNASPQTLTIAATSSSGQIKKNFVLEQ
                     RKPASAGFKGFSPSDVIYLIMTDRFADGDPANDSIGGNDGGRAAPHGWHGGDLKGIEE
                     HLDYLQKLGATALWLTPVTSNAGMKDSYHGYGATDLYATDPHFGSIEDYQHLVNALHA
                     RGMKILMDIVPNHVGVAIPWANDPPAPEWFHGTLEHHLIAKSWFQYLSDPHASASNRK
                     VVTEGWFTDSLPDLNQENPLVAKYLTQNTIWWIETAGIDGLREDTFPYVGRHFWAEFH
                     QQIHALYPNMKTVGEVVPSSPPVAAFFAGGVSHSGIDTGLDTPFDDATYFAIRDVLLR
                     GKPMTTLEETLGEDWLYPHPERLVTFFGNHDTMRFLSEPGANIAKLKVAFGLVATLRG
                     TPEIYSGDEIAMRGGYDPDNRHDFPGGFASDKTNAFVESGRTGEQQDVYAWVQGLLHL
                     RAREIALTQGQQQNIFADDTVFAFVRGTKLDSGCRNGEDRLLIMTGRNESPRKVSLKT
                     AQTALEGCKRYMQLWPAPSQAQLRDTGGELTVDLPANGFAIYRAQE"
     gene            complement(4104..7475)
                     /locus_tag="VFA02_00920"
     CDS             complement(4104..7475)
                     /locus_tag="VFA02_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08436.1"
                     /translation="MDASLWVGLACLFVLFGAGRAFSQTDTARIQGTVLDQSGAAIPN
                     ATITLTNTDTGITQTATSDSAGNFSFNALVRGNYSAEVTAQGFTTQTQKLVLEVSQVQ
                     ALNFQLQPGAVSTTVTVTSAAPIVDTASSSLGDVIQGRQTTELPLNGRNFTQLALLTP
                     GVTRGQYGDISSGVNGNAETFRNQENGGAAISTNGLRQQANNYILDGIDNNEALVNTI
                     VFFPNVEATQEFRVNTSVAPAEFGRAGGSIVQTSIKSGTNDIHGSAFWFARSSLFDAN
                     PNYQFLGASHTPALPYRRNQFGGSAGLPLVRNHLFLFGDYQGTREYTPLNAQILTVPT
                     RLMRQGNFSELLGQSNVINLAGSSITGCQQHTLANGTVVSSANPPNGAIFDPITCSQF
                     DYNGQPNVIDPSRLNKAAVNYLNAYPLPNIPGTNNGTENNYRTIRKQVTHYNTFDTRL
                     DWVPNVNNQLFGRFSYDQSQFTRTSEFATLPAGFASGSNNVHARGIALGYTRTFTPNV
                     LNELRVGYNRYTLTNVPVFSNIPVSASLGIVNANRTPQLGGGALIGGYQNQLEYTGDY
                     GTYAVPENTYEVTDSLTFNHGTHSFKFGGTAIRRDVAFFRPIAGKGYFQIGNGDFTGY
                     EVSELLAGFVDNYSIGAQNGFFGTRNYEWGIFGQDDWKITRRLTLNLGVRYDIITYPT
                     EEHNRQAALNPVTGHIDLAGVNGVPRSIVDTDFNNVAPRFGFAYDVFGNGKTALRGGY
                     GIFYFLDRGGIDNQFGQQVPFGGSVEYAAANGYRITFTGQGPLDENDNRLATNPLPLP
                     GYPNFDPNNPPAGVNIFTTNRHNQIPMVQQYDLQVQQQIGPQTVFSLAYVGNRTVHLA
                     TGYNYNTLKLDGSPQLFPNLGQIVAEFNNGVSHYDSLQASINHRYSKGLTLTGSYTWA
                     HNLDTSDGYLGFYAVSPLYVFDTKLNKGNSSLDQRNVFVASALYELPFGRGRQFGNSW
                     NRGLDVIAGGWQLNTIVQAETGTPVTVVYPQYGGNFSIRASASRPIAEPHSISGHWFD
                     PTPFYQPAPGTDGNVSRNYLHGPGLAEGDVSLFKNLSITERVKAELRAEAFNVTNTPQ
                     FTNPDSNPQDGNFGKITATRQYSERQLQMAVRFTF"
     gene            7787..>8908
                     /locus_tag="VFA02_00925"
     CDS             7787..>8908
                     /locus_tag="VFA02_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZU08437.1"
                     /translation="MQAAEQSDQPPVLPEKYTFGLFEADLKTGELRKNGKRIRIQGQP
                     FRVLSILLEHHGEIVSKEQIQKRLWGDNTTVDFDHSLGIAINKLRDALGDSADNPRFI
                     ETLARRGYRFIAPIKEIHSAEAGPQSEAISSIAASATPVEEKHPTQYSGTMAWAVLTL
                     CFAILTLWLWLRTPAIQGPLRISAVTHHGKVLATQLDVENFSAMAVEGGRIYFSQLEG
                     GRPVLAEALTANGEVEDLRLPSEIVAPLICSISKDGSRLIVRNHLAREPEQPLWIVPA
                     LGGQAERFGNVLAHDAAWMPDGQHILYATGHALFLAREDGQESQQLVTLPGRAFWLRW
                     SPDGKLLRFTVLDPQTGDPSLWEYSASSRKLRRLLDGWSR"
BASE COUNT         2016 a         2568 c         2300 g         2024 t
ORIGIN      
        1 ctccgcacac tgtatccata cgttcagctt gtgcaaagcc acatatttgg cttctccgga
       61 gaaatcgccg gcacactttc ccaggccatg atgtacctgc aatttcctct ctatggtctg
      121 ctgatgtccc tgctgctgcg cgccaggggc ttcatgaccg cgctgctcac aacagccatc
      181 gtgcatttcg gcgccgtcgt cattgccgta gctctcgcgc atctataaag tagaaagttc
      241 ccaatggccg acgccgtaaa gatcattgaa tgcccgcgcg acgcatggca aggtctgcca
      301 aagaccatcc ctgccgaagt caaggccggc tacctgcgtg cgctcatcgc cgcaggattc
      361 aggcacttgg acgctgtttc tttcgtctcg ccaaaggccg tcccgcagat ggccgactcc
      421 gagcaggtac tcgaatacct cgacccaccc gacgatgttg aaatcatcgg catcgtcgtc
      481 aatcagaaag gcgcagagcg tgccattgcc accgaagccg tccgcacgct cggattcccc
      541 tattccatct cctctgaatt tctgaagcgg aatcagggac agactccgga agagtcgctc
      601 gacgtgcttg aaaccattgg ccagtcagcc tacaaagccg gactcaacct cgtcgcctac
      661 atctccatgg catttggaaa tccgtacaat gacgcctgga gcatagatga ggtcatcgcc
      721 gcctgtgacc tgctcgccga catgggcatc gagcagatct cgctggcgga caccgtcggc
      781 ctcgccactc ccgatgaagt gtccctcctt gtcagcgctg tgctttccgc aaacgatggt
      841 ctggaaatag gcgtccacct gcacgcgcgt cccgaagagg tcgcggcaaa agtagcagcc
      901 gcatatcgtg ccggatgccg ccgcttcgat atggccatcg gtggtctcgg aggctgtccc
      961 tttgcgcagg atgcactggt cggcaacatc tcctctgagc tggccatcgc agagctggag
     1021 cgcctcggcg ccgaacttcc gtccttccaa cccttaaaca gcatcctcgc agcaaaccgc
     1081 gcgatcgcag aaaaattcgg cccggcgaaa caatgaaact ttttatcgga actcagtttc
     1141 ggcctacttt gaaatccctt ccagcggaga actcgccgtc gcatacagtt tgcgcggcat
     1201 cctccctgcc agatacgcct gccgcccagc caggaccgcg tgctgcatgg cttccgccat
     1261 cagaactgga ttggcggcag ccgcaatcgc cgtattcatc agcacgccat cagcacccat
     1321 ctccatcgcc agggccgcat cagaagccgt acccacaccc gcatctacaa tcagcggaac
     1381 ttcagtgacc atctcgcgca gaatctgaag tgtggcgcgg ttttgaatgc ccaatccgct
     1441 gccaatggga gcagccagtg gcatcacggc agatgctcct gcgtcaatca gtcgttttgc
     1501 aaatacaatg tcatcagatg tatatggcag aactgtaaag ccttctttta ctaatatgcg
     1561 ggtggcttcc agagtggctt gtacatcagg ataaagcgtt gcctcgtcgc cgatgacttc
     1621 gatcttgacc caatccgaga tacccacttc ccgggccaga cgcgcggcgc gaatggcctc
     1681 ctcggccgtg taacagccgg ccgtgttggg cagcagaaaa taacgctgcg gatcaatata
     1741 gtcgagcaat gattcctttg agcggtcgag attcacacgt cgcaccgcca ccgtgaccat
     1801 ctccgccccc gaggcctcaa tggcggcctg cgtttccgga ccgtccttat atttgcctgt
     1861 gcctacgatc agccgcgact gaaacgcctt ccctgcaatg accagtggtt ccatacctct
     1921 attgtagggc cgcgccatcc tattcctgtg cgcggtagat tgcgaacccg ttcgccggca
     1981 aatctactgt aagctcccct ccggtgtccc ggagctgggc ctgggagggt gcaggccaca
     2041 attgcatata tcgcttgcat ccttcaagcg cagtttgcgc agtcttcaaa gaaaccttcc
     2101 gcggcgattc attcctcccg gtcatgatca acaagcggtc ttcgccattt ctgcagcccg
     2161 aatccagttt cgtcccgcgc acaaaagcaa aaacagtatc atcggcaaaa atgttctgct
     2221 gctgcccctg cgtcagcgca atttcgcggg cgcgaagatg aagcagcccc tgcacccacg
     2281 cataaacatc ctgctgttcc ccggtgcgtc ctgactcaac aaaggcattg gtcttgtcgc
     2341 tggcaaatcc gccaggaaaa tcgtgccggt tatcaggatc gtagccacct ctcatcgcaa
     2401 tctcatctcc gctgtaaatt tcaggagttc cgcgcagagt ggccaccaga ccaaatgcaa
     2461 ccttcagttt tgcaatgttc gcgcccggct cagacaagaa acgcatcgta tcgtggttgc
     2521 cgaaaaacgt caccagtctt tctggatgag gatagagcca atcttcaccc agtgtttctt
     2581 cgagggtcgt catcggcttg ccgcgcagca acacatcgcg gattgcaaaa tatgtggcat
     2641 cgtcaaaagg cgtatcaagc cccgtatcaa tgcctgagtg cgatacacct cctgcaaaaa
     2701 acgcagccac aggcggactg gatgggacca cctctcccac agtcttcatg ttcggataca
     2761 gcgcgtggat ctgctgatga aattccgccc agaaatgacg gccgacataa gggaaagtat
     2821 cttcgcgcag accgtcaatc cccgctgtct caatccacca gatcgtgttc tgggtgagat
     2881 actttgccac cagcggattc tcctggttca ggtcaggcag cgaatccgta aaccagcctt
     2941 cagtcacaac cttcctgttg ctggcagaag cgtgcggatc ggacaaatat tggaaccacg
     3001 attttgcaat caaatggtgt tccagcgtcc cgtgaaacca ctctggcgct ggcggatcgt
     3061 tggcccacgg aatcgccacc cccacatggt tcggcacaat gtccataagt atcttcatcc
     3121 cacgcgcatg aagtgcgttg accaaatgct gatagtcctc aatcgaacca aagtgtgggt
     3181 ccgtggcata gaggtctgtc gccccgtagc cgtgatacga atccttcatt cccgcattgc
     3241 tcgtaacagg cgtaagccac agcgcagtcg cgccgagctt ctgtaaataa tcaaggtgct
     3301 cttcaatgcc tttgaggtcg ccgccgtgcc agccatgcgg agcagcgcgt cctccatcat
     3361 tgccgccgat gctgtcattg gcagggtctc catcggcgaa acggtccgtc atgatgagat
     3421 aaatgacgtc gctgggtgaa aagcctttga atcctgccga agcaggcttt cgctgctcta
     3481 acacaaagtt tttctttatc tgtcctgaag atgacgttgc tgctatggtc agagtctggg
     3541 gactcgcatt cgtagtgtcc agccacagaa aggcccagtg tccattttgc gaaatctggg
     3601 tctttgtcag cccaacattt cgtccgcgaa caagaaaatg cgctccgttc aagtggtttc
     3661 catgcagcag aagcataggg ctgggcatat ggatccacca gttcgggggc tcaatcttgt
     3721 ccacgcaagg gccggaagtc tgctcttccg cgcaactctg cgcctgggta aatgaatgat
     3781 tgttccgggc ggttacaggc agattgccgt cctgagcata ggcgtgcagc gccggagtcg
     3841 aaggacctgc gtttacggtg ctcaaatccg cacgattcaa cctttgcgca accgcaaatc
     3901 cgcaaaaaga gaaagagaca acgccagcaa caacacactt cagcagcgtc atgaataatt
     3961 ccttatcggg aaaaagagcg ccacgccgga agagccggcg cagcgctgta aaaaatgact
     4021 aaaattcagc catgaagaat ctggatgttg gcccccccgc catggttttc cccttggatt
     4081 ggatggcccc ttccagatac cttctaaaac gtgaatcgta ccgccatctg cagctgccgc
     4141 tcagaatact gacgcgtcgc cgtgatcttg ccgaaatttc cgtcttgcgg attcgaatcc
     4201 gggttcgtga attgcggtgt atttgtcacg ttaaaggcct cggcgcgaag ctctgccttc
     4261 acacgctccg taatggaaag gttcttgaag agcgaaacat ctccctctgc caacccgggg
     4321 ccatgcaaat aatttctgct tacgttcccg tccgttcccg gggccggctg gtagaacggc
     4381 gtgggatcga accagtggcc tgaaatgctg tgcggttcag caatggggcg gctcgcactt
     4441 gcgcgaatac tgaagttgcc accatattgc gggtaaacca ctgtgacggg cgtcccggtt
     4501 tctgcctgga caatcgtgtt caattgccac cctccggcta tcacatcaag cccacggttc
     4561 cagctattac caaactgcct gccgcgtcca aaaggaagct catacagagc gctggccaca
     4621 aagacattcc tctggtccag gctcgaattt cctttgttga gttttgtatc gaacacgtac
     4681 agaggactta cagcatagaa gccaagatat ccatcgctgg tgtcgagatt atgcgcccat
     4741 gtatacgaac cggtaagcgt cagccccttc gagtaacggt ggtttataga cgcctgcaag
     4801 ctgtcatagt gggagactcc attgttgaac tcagccacaa tctgtcccag gttggggaaa
     4861 agctgcgggc tgccatcaag tttcagtgtg ttgtagttgt agccggttgc cagatgcaca
     4921 gtccggttgc ccacgtatgc aagtgaaaaa accgtttgcg gccctatctg ctgctgcact
     4981 tgcagatcat actgctgcac catgggaatc tgattgtgcc ggttcgtcgt aaagatgttt
     5041 acgcccgctg ggggattgtt cggatcaaag ttcgggtagc cgggcaatgg cagcggattg
     5101 gtcgcaagcc tgttgtcgtt ttcgtccagc ggaccttgtc cggtaaatgt aatgcgataa
     5161 ccattcgcag cggcatactc gacgcttcca ccaaaaggta cctgctgccc gaactggtta
     5221 tcaattcctc cgcggtcgag gaaatagaag atgccgtagc cgccgcgcag agcagtcttg
     5281 ccgttgccga aaacatcata ggcaaaccca aagcggggcg ccacattgtt gaagtccgta
     5341 tctacaatgc tgcggggaac accgtttacg ccggccaaat ctatatggcc cgtcacggga
     5401 ttcagcgcag cctggcggtt atgttcttcg gtcggatagg taatgatgtc gtaccgcaca
     5461 cccagattca gagtaaggcg ccgcgtgatc ttccagtcgt cctgcccaaa gatgccccat
     5521 tcataattgc gtgttccgaa aaagccgttt tgcgcgccga tgctgtagtt gtctacaaag
     5581 cccgccagca gttcagaaac ttcatacccg gtgaagtcgc cgtttccaat ctggaaatag
     5641 cctttgccgg caatgggccg gaagaacgca acatcgcggc gaatggctgt gccaccgaat
     5701 ttgaacgagt gcgtgccgtg gttgaatgtc agggagtccg taacttcgta ggtgttttca
     5761 ggaacagcat acgttccgta atcacccgtg tactcaagct ggttctggta gccgccgatc
     5821 aatgccccgc cgccaagctg cggagtgcgg ttggcattca caatgcccag gcttgcggaa
     5881 acaggtatgt tgctgaacac tggaacattg gtcagtgtgt aacggttata tcccacgcga
     5941 agttcattca gaacattcgg agtaaacgta cgcgtataac ccagcgcgat tccacgcgca
     6001 tgcacattgt ttgagcctga cgcaaaacca gcaggtaaag tggcgaattc tgacgttctt
     6061 gtgaactggc tctggtcata gctgaaacgt ccaaaaagct gattgttgac gttcggcacc
     6121 cagtcaagcc gcgtatcaaa cgtgttgtaa tgggtgactt gcttccggat ggtccggtaa
     6181 ttgttctctg tcccgttgtt cgtccccgga atattcggca gtggataagc attcaggtaa
     6241 ttcaccgctg ccttgttcag acgggacgga tcaattacat tcggctgacc gttgtaatcg
     6301 aattgggagc aggtgatggg gtcaaaaata gcgccattgg gcggattggc tgaactcacc
     6361 accgttccgt tggccaaagt atgttgctgg caccctgtaa tcgagctgcc cgcaaggtta
     6421 atcacattgc tttgccccag cagttccgag aaatttccct gccgcatcaa ccgtgtcggt
     6481 acagtcagga tctgcgcatt cagtggcgta tattcgcggg ttccctggta atcgccgaac
     6541 agaaaaagat gattacgcac cagaggaagg cctgccgatc cgccaaactg gtttctacga
     6601 taaggtaatg cgggtgtgtg gctggctccc agaaactgat aattgggatt ggcatcaaac
     6661 aaactgcttc gggcaaacca gaatgcagaa ccgtgaatgt catttgttcc ggatttaatg
     6721 gaggtctgca caatggagcc gcccgcacgc ccaaattccg ccggggcaac gcttgtgttt
     6781 acccgaaact cctgcgtcgc ttccacatta gggaagaaca cgatggtatt caccagcgct
     6841 tcattgttgt caattccatc cagaatgtaa ttgtttgcct gctgacgaag accattggtg
     6901 gaaattgccg cgccgccatt ctcctgattg cgaaacgtct cagcattccc attcacgccg
     6961 ctggaaatat cgccatactg tccgcgcgtg accccgggcg tgagcagggc cagttgggta
     7021 aagttgcggc cgttcagagg cagttcggta gtttgcctgc cctggatgac atctccaagc
     7081 gaagaactgg cggtatccac aatcggcgcc gcactcgtca cagtcaccgt ggtcgaaact
     7141 gcgcccggct gaagttgaaa gttcagcgct tgcacctggg agacttccag caccagcttc
     7201 tgggtttgtg tcgtgaatcc ctgcgcggta acctcagccg agtaatttcc ccggacgagc
     7261 gcgttaaagg agaaattccc cgcactgtca ctggtggccg tttgagtaat accggtatcg
     7321 gtattggtca gggtgatagt ggcgtttggg atggccgcgc cactttggtc aagcaccgta
     7381 ccctggatgc gggccgtgtc cgtttggcta aatgcccgtc cggccccgaa cagtacgaac
     7441 aggcaagcca ggccaaccca aaggctggca tccatagata gtctatgaaa aaaatgtgtc
     7501 gctttttgca tcgaaatgcc tccgaaagtg agggtcttac tgtgtttttg cttttacccg
     7561 acagacgcct tgaaatgctt acaatgcact cccgtccggg ccgacataca ccatcctctc
     7621 tctactcaac gattggcaat acacagagtt tctatttctt atcaaatatt tatctccttt
     7681 caaatcgctt attttcaatt atttatacag ataaatctct tcaccgacgg gattgactca
     7741 ggcccttcgg cgggagtata aaccgacagg agatgttcgt tttttcatgc aggcggcaga
     7801 acaatccgac cagcctccgg ttttgcccga aaaatacacc ttcggtctct ttgaagccga
     7861 cctaaagaca ggagaactgc gcaaaaacgg taaacgcatc cggatacagg gccagccatt
     7921 tcgcgtcctt tccatcctgc tcgaacatca cggcgaaatc gttagtaaag aacagattca
     7981 aaaaaggtta tggggagaca acactaccgt tgatttcgac cacagccttg gcattgccat
     8041 caacaagctg cgcgacgcac tcggcgattc agccgataat ccacgcttta ttgaaaccct
     8101 ggctcgccgc ggctatcgct tcatcgcccc gatcaaagaa atccactccg cagaagctgg
     8161 gccacaaagc gaagcaatct cctccattgc cgcctctgcc actcccgtcg aggaaaagca
     8221 tccaacccaa tacagcggaa ccatggcttg ggcggtgctg actctgtgct tcgccattct
     8281 gacgctctgg ctttggctcc gcacgcctgc aatacagggc ccgctgcgca tatccgcagt
     8341 aacacaccac ggcaaggtcc tcgccactca acttgatgtg gaaaattttt cggcaatggc
     8401 cgtggaaggt gggcgtatct atttctcgca gcttgaaggc ggccgccctg ttcttgccga
     8461 agcgctgacg gcaaatggtg aagtcgagga tctgcggctg ccttcagaaa tcgtcgctcc
     8521 cctgatctgt tccatttcaa aagatggatc acgactgatt gtcagaaatc atctggcgcg
     8581 cgagcccgag cagccgctct ggatcgttcc tgcactgggt ggacaggccg aacgattcgg
     8641 aaatgtcctg gcccatgatg ctgcctggat gccggacggc cagcacattc tctatgcaac
     8701 cggacatgca ttatttctgg cgcgcgaaga cggacaggag agccaacaac tggtcacgtt
     8761 gccgggccgt gccttttggc tgcgatggtc gccggacgga aaattgcttc ggttcaccgt
     8821 gcttgatccg cagactggag acccttcgct ctgggaatat agcgcatcaa gccgcaagct
     8881 gcgccgcctg ctcgatggat ggagccgg
//
LOCUS       DASRBV010000022        15528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_355500, whole genome shotgun sequence.
ACCESSION   DASRBV010000022 DASRBV010000000
VERSION     DASRBV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355500"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFA02_00930"
     CDS             <1..478
                     /locus_tag="VFA02_00930"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523260.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZU08438.1"
                     /translation="FGMFVRALAYILANGPDGLRQTTEDAVLNANYIRKKLEDVYELP
                     YKTPSMHEVVFSDRKQLKNGVKTGDIAKRLIDYGFHPYTVSFPLIVPGALMIEPTESE
                     SREELDLFIDAMRQIAREVEENPQLVLNAPHSTRVSRLDETTAARKPVLRWKPKAE"
     gene            848..1792
                     /gene="cysD"
                     /locus_tag="VFA02_00935"
     CDS             848..1792
                     /gene="cysD"
                     /locus_tag="VFA02_00935"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZU08439.1"
                     /translation="MATAVYDSPETITASAARLTHLKALEAESIHIIREVAAEFQKPV
                     MLYSIGKDSSVMLRLAQKAFYPGPIPFPLMHIDTGYKFREMIEFRDNYTRELGLKLIV
                     WRNEEAIANGANPISLGTQKCCGYLKTQALLDGLRAYGFDAAFGGARRDEERSRAKER
                     IFSFRDTNGQWDPKNQRPELWNLYNGRIGPGESIRVFPLSNWTELDIWHYIHLENIPI
                     VPLYFAKERRMLVRGDSLIPIEQPFVKGLLGEEQWVRCRLRSLGCSPCTGAIRSDADT
                     LPKIIAELISFRSSERANRIIDHDQDGSMEIKKREGYF"
     gene            1792..3429
                     /gene="cysN"
                     /locus_tag="VFA02_00940"
     CDS             1792..3429
                     /gene="cysN"
                     /locus_tag="VFA02_00940"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HZU08440.1"
                     /translation="MAALSNVMDDLREDAVPFTIEEFLAAEQQKDLLRFTTAGSVDDG
                     KSTLIGRLLYDSRSVYEDQVKAVTRSSSGDASAIDFALLTDGLRAEREQGITIDVAYR
                     FFATPKRKFIIADTPGHEQYTRNMVTGASTAELAIILVDARKGILTQSRRHAFIASLL
                     GIRHVVAAVNKIDLVDFSEEVFFKHKKELQELGAKLGIADLLAIPISALLGDNVVTRS
                     ERTPWYGGPTLLEHLENVPIAEDVQHAAFRLPVQRVIRPNQDFRGFAGQIASGTVRPG
                     DKIIALPSGRSSRVKGIHTFDGDLEAAFAPQSITLTLEDEIDISRGDVIAHVEDAPSV
                     SSSFTASLVWLHEEPFVPGKTYQLKHTTQWVKAHLRVTQKLNIETLEPQASPVLHLNE
                     IGEAEVRTSRPLMLDAYRENRVTGSFILIDPANNATVGAGMVRQPSLDKSGRPQINGT
                     LLLLPGEVLVSVLEEKLTASGRDVVITRSEKPEVWRALLYAGLTVLVPSKLHAQAEII
                     NLGPDAHELQRRVVPRQDGLKAQAEALLQELENESEF"
     gene            3413..4102
                     /locus_tag="VFA02_00945"
     CDS             3413..4102
                     /locus_tag="VFA02_00945"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZU08441.1"
                     /translation="MSQSFEVAGLTEKTGEAQALVAKYVTGLATECVTCSFQAEDMVV
                     LDMVRGLFPDVPVLFLDTGYHFAETYAYRDRMASEWKLNLINLLPRQTVAEQEAQFGI
                     LHQTAPDQCCKLRKVEPLFSALADYKTWFTGMRREQAKTRANMQAIDHFKLPSGHELI
                     KISPLADWTTRDVWYYAQERNIPLLPLYEKGYSSIGCEPCTSLPSDPNDPRSGRWAGR
                     KTECGIHIQAQ"
     gene            4113..4949
                     /locus_tag="VFA02_00950"
     CDS             4113..4949
                     /locus_tag="VFA02_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU08442.1"
                     /translation="MELLFGFLIAFAIAITGVGAGTVTAPALVLFLHVPLPIAVGTAL
                     VYASVVKLIVVPVQIIRNQVVWKILGMMLVGGLPGVLVGVVLFRRVVGHGFQAPLYFA
                     LGSIIVFASGLHFYKAFRARKEIEARKERRRWLAFLMLPIGAELGFSSSGAGALGTVA
                     LLGLTNLTATEVVGTDLAFGLCLSILGGGLHLLVHTPDTALLWKLLIGGVAGAIAGTL
                     VAPRLPHKQLRLVLTAWLLLIGLQFCYQAVRPPADASQHEKRVAVSGAVQNAMLSRPH
                     SQ"
     gene            complement(4969..6219)
                     /locus_tag="VFA02_00955"
     CDS             complement(4969..6219)
                     /locus_tag="VFA02_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08443.1"
                     /translation="MTEQSSPVIVEHLFRRQAGRIISWLAGLLGPQHLQLAEDAAQEA
                     MLRALESWSFHGVPEKPEAWLFRVAHNYAISVLRRDRKSGELSEVQDIKAGPASLDHP
                     ELDEQLRDDELRMVFMCCHPDLPENARVALGLQLVGGFSAGEIARVFLADERTIAQRL
                     VRAKRQMREQQVPLSMPSELHDRLDSVLHVIYLMFSSGYAAHSGEELMRRDICMEALR
                     LGQLVAGASLAAPRVDALVALMALQAARLPARTDASGDLVLLEEQDRNLWDDHLIGMG
                     FYFFDRSIRGNEISGWHIQAAIAAEYAKAKTAHFICWQTILENFDHLMAVNPSPVVAL
                     NRAVVIMKVHGAEAALAEILPLEAHASMRGYYLLPAVKGRLLMELGRHAEAARAFHAA
                     LACDCSQPERRFLEKQLSLARASE"
     gene            complement(6220..6564)
                     /locus_tag="VFA02_00960"
     CDS             complement(6220..6564)
                     /locus_tag="VFA02_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZU08444.1"
                     /translation="MPQYMILLRNDGDPFSRLSPEEAQRTTEKYFAWRQKPFTVGGAR
                     LDHQGRVIRKQNGSVSVSDGPFSEGREVLGGYYAIEAANFDEAVKFALTNPHIDFGTI
                     EVREVVPRPADK"
     gene            complement(6811..8454)
                     /locus_tag="VFA02_00965"
     CDS             complement(6811..8454)
                     /locus_tag="VFA02_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HZU08445.1"
                     /translation="MRARRTFLLLIAALCPFHLSAQSRQVTITVHADQTEGPYIPIWN
                     YFGADEPNYIYAPNGLKLLNELSALSPVPVYFRVHNLFTTGNGQGSLKWGSTNVYTEK
                     PDGTPVYDWTITDKIFDAFQTAHIRPLVEIGFMPQALSTHPQPYRHNFPNGDVFTGWS
                     YPPKDYAKWSNLVVSYVQHLKQRYGNDVHGWLWEVWNEPDIAYWHGTPEEYDKLYDVT
                     AAAIRKVLPDARIGGPEATGVHPGRSERFLRQFLEHCANGKNYATGQTGSPLDFISYH
                     PKGSPKFINSHVQMNIGEQLRAVDYGMRVIASYPQWKNTPIILGESDPEGCAACKGSQ
                     NGYRNGPLYGVSVAEAIARTYELARKNGMTVLGSVTWAFEFEDQPYFAGFRELATNGI
                     DKAVLNVFRMYGKLGGNWVRVESTGAVPVEDVLQHSVTAQPDVSAIATQNGRELNVLL
                     WNYHDDDVTAPPAEISVSVFGLPAKGVRVQEFRMDRDHSNAYAIWQKMGSPQHPSPAQ
                     QQQLEQAGKLEMIKQVRLRDAGMHINLPRQGVSLLHFYW"
     gene            complement(8512..9123)
                     /locus_tag="VFA02_00970"
     CDS             complement(8512..9123)
                     /locus_tag="VFA02_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HZU08446.1"
                     /translation="MASLLFIESSPRATSVSSAVAREYVTKWQQQNPTGTVVRRNVAA
                     NPVPHITEAWIAAVFTPAENRTPEQKQLLALSDTLIGELEAADTIVIAVPMHNFGISA
                     HLKTWIDQIVRAGRTFAYSSEGYKGLLDGNKKVIVITARGGAYPAGTPVGAFDQQEPY
                     LRAILGFLGLKDIHFIHTENQNRGQELAQQGFHAGVEAALALA"
     gene            complement(9291..10034)
                     /gene="kdsB"
                     /locus_tag="VFA02_00975"
     CDS             complement(9291..10034)
                     /gene="kdsB"
                     /locus_tag="VFA02_00975"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZU08447.1"
                     /translation="MAEKQRILGVVPARLASTRLPRKVLREIAGEPMLAWVYRAARAC
                     PRLDEVLIATDSDEVMEFAHSRSFPAMFTPAGCASGSDRVYAVAQLVPADIYVNIQGD
                     EPMLRPEHLDALLKLMERPEVEVATISTPCAPGHIQDPNAVKVVTAANGRALYFSRAA
                     IPYDRDKTGNVPFEKHLGIYAYRKAALDRFPTLPSRRLEQIEKLEQLRFLENAIDIYV
                     ASTPYDTIGVDTEEDRLAVEKLLLARQLK"
     gene            10168..10244
                     /locus_tag="VFA02_00980"
     tRNA            10168..10244
                     /locus_tag="VFA02_00980"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10202..10204,aa:Met,seq:cat)
     gene            complement(10255..11403)
                     /gene="tsaD"
                     /locus_tag="VFA02_00985"
     CDS             complement(10255..11403)
                     /gene="tsaD"
                     /locus_tag="VFA02_00985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZU08448.1"
                     /translation="MRTGLILGIESSCDETAAAVVREGRETLSNVVASQIATHAPYGG
                     VVPELASREHLRNIMPVVRTALAEANTSLQELDAIAVTEGPGLAGALLVGISYAKSLA
                     FALNKPLIAVNHLEGHIHAVLLEQTQLREEPLPLPALALVVSGGHTHLYLARHQRESW
                     RYKNVGHTVDDAAGEAFDKVAKLLGLGYPGGPWMDALAPHGDPKAVPFSFAQIKPKVH
                     RKETLREEFSQQFFFSFSGIKTAVLRYVQTHEMSASIEARRAALARIPHAKPADALSL
                     CDKETLDLIASFQRAVVDDLRRKTFQAAETFDAASILVSGGVAANRELRVRFTAEAAD
                     RGLPIGFPSLSLSTDNAAMIAAAAWPKFLAQEFAPPELTAVASLALGK"
     gene            11491..12897
                     /locus_tag="VFA02_00990"
     CDS             11491..12897
                     /locus_tag="VFA02_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZU08449.1"
                     /translation="MTKTHAQLAAERIVERLRAEGYDAYFAGGCVRDLLLGHAPADFD
                     VATSARPETVLGLFRRTFAVGAHFGVVLVADEVDGEQIVTEVATFRNDGSYSDGRRPD
                     EVSFSDSAQEDVLRRDFTINGMLLDPRMLTETGNMEAAVLDYVGGRADLSAGMVRAIG
                     EPERRFREDKLRMLRAVRFAARFEFAIEQVTETAICRHAGEISQVSNERVRDELTRML
                     TEGHARRAFELLDQTGLLKQVLPEIDRLHGVEQPPEYHPEGDVWAHTLLLLEKLPAGV
                     SPTLAWGALLHDVGKPATFERAPDRIRFNGHVEVGSRIAAEICRRLRFSNEQTAQILS
                     LVENHMRFGDVMRMKESTLKRFFRLPKFSEHLELHHMDCLSSHGDLTLYNFAKQRYET
                     MPEEVVKPRLLVTGADLIAVGYKPGPRFKEMLSLAEDAQLEGHIRTKEEGLKLVRDTF
                     GGLAADPEDERLRHPRGA"
     gene            13238..>15528
                     /locus_tag="VFA02_00995"
     CDS             13238..>15528
                     /locus_tag="VFA02_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU08450.1"
                     /translation="MKQTTAGMASRDLGALSRHVPFTRLCWSLAGRTIAVLVCIVFLF
                     ASAMRGQLTTGSLSGTVADQSGAVVPNARVTLTNEASHDTRLTTSNSAGYFSFAAVQP
                     GSYTVTIDANGFNIWKQSGIPMNPGDIREVSGIKLTTGGGTTTVEVQAAAAQILPTDS
                     GERAAVLSSKDIEKLALEGRNVSELLKVLPGVSMTSGGTSGGTSMDFSASAPTGSTVG
                     NGLTTSGAPYRGGTAYMLDGANIIDPGCNCYSIAVPNPDMTQEVKVQGSFGADSPNGP
                     EIVNVISKSGGSQYHGEGYFYARNQVLNANTWLNNNSGIKRPNGEYYYPGGNLGGPVK
                     IPGTSFNANNKLLFWVGYEYYKQTLPAAQPFESYIPSAGMMAGDFTPTGDGNAALCPN
                     GFSSSANNWCNDLSGAVAPDGTPITGGVIPAKYLDAGAAALMKMFPKPNANPATTPGG
                     FNYIQPITNEQNGYVFRARVDYNLNDNTKFFATYQQGNTTFITPGHVWWTPSYTIPYP
                     GGNITSPNTSRVVTANLVNIITPTLTNEFVFGWGWDDGPFNPSKLSAIYKSALGYPYA
                     TVYNTASLVAPSINSAGAQTFPDISQPAFFGTSGSYPLLKAIPSFADNVTKVWRSHTF
                     KFGAFTELVGNRQGVWSYPNGVLSFGSSPESNNVAGSQVASGTKIGSSNPTANLVMGI
                     ASGFSQNNYVPVQDMAYRTTAGYIMDNWHVNSRLNLNLGVRFDHIGRWYDRQGTGLAV
                     WLPQLYQQDVAANQTAGSMVVEYP"
BASE COUNT         3300 a         4075 c         4726 g         3427 t
ORIGIN      
        1 cttcgggatg tttgtgcgcg cgcttgcgta cattctggcg aacggcccgg atggtctgcg
       61 ccagaccaca gaagacgcag tgctgaatgc aaactatatc cgcaaaaagc tcgaagatgt
      121 ttacgaactg ccctataaga caccttcgat gcatgaggtc gtcttcagcg accggaagca
      181 gctaaagaac ggcgtcaaga ccggagatat cgccaagcgg ttgattgatt acggcttcca
      241 tccctatacg gtgtccttcc cgctgattgt gcccggtgcg ctgatgattg agccgaccga
      301 gagcgagtcg cgtgaggagc tggacctgtt tattgacgcg atgcgccaga ttgcgcgcga
      361 ggtggaggaa aatcctcagc ttgtgctgaa cgcgccgcat tccacgcgtg tatcacgtct
      421 ggatgagaca accgcggccc gtaagcctgt gttgcgctgg aagccgaagg cggagtaatt
      481 tcagcgcgca aagagaggca gagaagccag agcaggatgg gctaaaccta cggatggctg
      541 agattcttgc tccctacggc cgtcagaatg acggactaca tatttacgaa aaatgttata
      601 agaattgaca atccagccct ctgtgcgaga tgctaaatag cacatggcat ttatgctctg
      661 taccttcgct tgtcgcatgg actgtcgctg ttgttgcagc atcagcgcgt gtcggcgggg
      721 tatggacgct atttaagtag aatccaaggt ttttctgtaa aaccagaccc gcagccagac
      781 atctgagcgc gggttttctt gttttcccgc ctctttcatt cacccaagac aggaaatttc
      841 agcagccatg gcaaccgcag tctacgattc gccggaaact atcactgcaa gcgcagcgcg
      901 cctgacgcac ttgaaagcgc ttgaggcaga gagcatccac attatccgcg aggtagcggc
      961 cgagttccag aagccggtca tgctttactc catcggcaag gactcatcgg tgatgctgcg
     1021 cctggcacag aaggcgtttt atcccgggcc cattccgttt ccgctgatgc atattgatac
     1081 gggctacaag ttccgcgaga tgatcgaatt tcgcgacaac tacacccgcg agcttggcct
     1141 gaagctgatt gtctggcgga atgaagaagc gattgcgaat ggcgcgaacc cgatttcact
     1201 ggggacgcag aagtgctgcg gatacctgaa gacgcaggcg ctgctggacg gcctgcgggc
     1261 ctatggtttt gacgcagcgt tcggtggagc gagacgcgat gaagagcgtt cgcgagccaa
     1321 agagcgcatc ttttccttcc gcgatacaaa tgggcagtgg gacccgaaga accagcggcc
     1381 agagttgtgg aacctttata acggccgtat tggtccgggt gagagcatcc gcgtgtttcc
     1441 gctttccaac tggacggagc tcgatatctg gcactacatc catcttgaaa atatcccgat
     1501 cgttccgctg tattttgcga aagagcggcg gatgctggta cggggcgaca gcctgattcc
     1561 gattgaacag ccatttgtta agggccttct cggagaagag cagtgggtgc ggtgccgtct
     1621 gcgctcgctc ggttgcagtc catgcacggg ggcaatccgc tcagacgcgg acacgctgcc
     1681 gaagatcatt gccgagctga tttccttccg ctcgtcggag cgcgcgaacc gcattattga
     1741 ccatgaccag gacggctcga tggagatcaa gaaacgcgag gggtatttct gatggccgct
     1801 ttgtcgaatg tgatggatga tctgcgggag gatgcggtcc cattcacgat tgaagagttt
     1861 ctagccgccg aacagcagaa agacctgctg cgttttacca cagcgggcag tgtagacgac
     1921 ggcaagtcta cgctgattgg ccgtttgctt tatgattcgc ggagcgtcta cgaagaccag
     1981 gtaaaagcgg tgacgcgcag cagttccggc gacgcttctg cgattgattt cgcgctgctg
     2041 acggatggtc tgcgcgcgga acgcgaacag ggaatcacga ttgacgtggc ctaccgcttt
     2101 tttgcgacgc cgaagcgcaa gttcattatt gccgatactc cggggcatga gcagtacacg
     2161 cgcaacatgg tgacgggcgc gtccaccgcg gagctggcga tcattctggt ggacgcgcgc
     2221 aagggcatcc tgacgcagtc acgccgccat gcatttattg cttcgctgct tgggatccgt
     2281 cacgttgttg ccgcggtcaa caagatagat ctggttgatt tttctgaaga ggtgtttttc
     2341 aagcacaaga aagaattgca ggagcttggg gcgaagctgg gcattgcaga cctgcttgcg
     2401 attcctatca gcgcgctgtt gggcgataac gtagtgaccc gcagcgaacg cactccatgg
     2461 tatggcggcc cgacgctgct ggagcatctt gagaatgttc cgattgccga agatgtacag
     2521 catgcagcat ttcgtcttcc ggtgcagcgt gtgattcgtc cgaaccagga tttccgcggg
     2581 ttcgcggggc agattgcatc gggtacagta aggcccgggg acaagattat tgccttgcct
     2641 tcagggcgaa gcagccgggt gaaaggcatt catacattcg atggagatct ggaagcagct
     2701 tttgcgcccc agtccatcac ccttacactt gaagacgaga ttgacatcag ccgcggagac
     2761 gtcattgcgc atgttgagga cgctccttca gtttccagca gctttacggc gtcgctggtc
     2821 tggctgcatg aggagccctt cgttccgggc aagacttacc agttaaaaca cacgacacag
     2881 tgggtgaagg cgcatctgcg tgttacgcag aagctgaaca ttgaaactct ggaaccgcag
     2941 gcatcgccgg tgctccatct gaatgaaatt ggcgaggctg aagtcagaac gagccgcccg
     3001 ctgatgcttg atgcgtaccg cgaaaaccgc gtcacgggca gttttatcct gattgaccca
     3061 gcaaacaatg cgacggtcgg cgcaggcatg gtacggcagc cgtccctgga caagagcgga
     3121 cgcccgcaga tcaacggcac tctgctgctg ttgccgggag aagtgctggt gagtgtgctg
     3181 gaggaaaaac ttacggcaag cggtcgggac gtggtcatta cgcgcagcga aaagcctgag
     3241 gtatggcgcg cgttgcttta cgcgggcctg actgtgctgg tcccatcgaa attgcacgcg
     3301 caggcggaga tcatcaacct cggaccagac gcgcatgaat tgcagcgccg tgtggtcccg
     3361 cgtcaggacg gattgaaagc gcaggccgag gccttgttac aggagttaga gaatgagtca
     3421 gagttttgaa gtagcaggtc ttactgagaa gaccggagag gcgcaggcgc tggtcgcaaa
     3481 atatgtaacc ggtttggcta cagaatgcgt gacgtgcagc ttccaggccg aggacatggt
     3541 ggtcctcgat atggtgcggg gcctcttccc ggatgtacct gtgctttttc tggatacggg
     3601 ataccacttt gccgaaacct acgcttatcg cgaccgtatg gcttccgagt ggaagctgaa
     3661 cctgatcaat ctgctgccga gacaaaccgt tgccgaacag gaagcacagt ttggcattct
     3721 gcaccagacc gctccagacc agtgctgcaa actacggaaa gtggagccgc tgttttccgc
     3781 gcttgccgat tacaaaacgt ggtttacggg gatgcgccgc gagcaggcaa agacccgcgc
     3841 taacatgcag gcgattgacc acttcaagct gcccagcggt catgaactga ttaagatcag
     3901 tccgcttgcc gactggacga cgcgagatgt ctggtattac gcacaggagc gcaatatccc
     3961 gctgctgccg ttgtatgaga aagggtattc cagcatcgga tgcgagccgt gtaccagtct
     4021 gccttccgac ccaaatgatc cgcgatcggg acggtgggcc ggtcgcaaaa ccgagtgcgg
     4081 catccacatt caggcacagt aaagaaaccg gcatggaact gctttttggc tttcttatcg
     4141 cgttcgccat cgcaatcacc ggcgtcggag cgggcacggt caccgctcca gcgctggtgt
     4201 tgtttctgca cgtgccgttg ccaattgcag tggggacggc gctggtttat gcctcggtcg
     4261 taaagctgat tgtggtgccg gtgcaaatca tccgcaatca ggttgtctgg aagattctgg
     4321 gaatgatgct ggttggtggt cttccaggag tgctggttgg cgtggtcctc ttccgccgtg
     4381 ttgtcggaca tggatttcag gcgccgcttt atttcgccct gggcagcatt attgtatttg
     4441 cttccggact gcacttctac aaggcctttc gcgccagaaa agaaatagaa gcgcggaaag
     4501 agcgccgcag atggctagca ttcctcatgt tgcccattgg ggccgaactc ggcttttcat
     4561 cttccggagc cggcgcgtta ggaacagttg ccctgctcgg cctaacgaac cttactgcaa
     4621 ctgaagttgt cggcacagac cttgcttttg ggctatgcct ttcgatactc gggggcggac
     4681 ttcatctgct tgtccacact ccggacacag cgctgttatg gaagctgctg attggcggag
     4741 tggccggcgc gattgcggga acactggtgg caccccgtct tcctcataag cagttgaggc
     4801 tggtgttgac ggcatggctg cttctgattg ggttgcaatt ctgctatcag gcagtgcgtc
     4861 cgccggcgga tgcatcgcag cacgaaaaac gtgttgccgt ctccggcgcg gtgcagaacg
     4921 caatgctctc acggccccat tctcaataag tacggtccga ttcagctttt attccgaggc
     4981 cctggccagc gacaactgtt tttccagaaa gcggcgctcc ggctgggagc aatcgcaagc
     5041 cagggccgca tggaatgctc tggctgcctc ggcgtggcgg cccagttcca tcaacaggcg
     5101 gcctttcacg gccggaagca ggtaatatcc gcgcatcgag gcgtgcgctt ccaggggcag
     5161 aatttccgcc agcgccgctt cggccccatg cactttcatg atgacaaccg cgcgattcag
     5221 cgcgaccacc ggcgatggat ttacggccat gagatggtcg aagttctcca gaatagtttg
     5281 ccagcagatg aagtgggctg ttttggcctt tgcgtattcc gctgcaatgg ccgcctgaat
     5341 gtgccatccg gaaatttcat ttccgcggat ggaacggtcg aaaaagtaaa agcccatgcc
     5401 gatgaggtgg tcgtcccaaa gatttctgtc ctgctcttcc agcagaacaa gatcgcccga
     5461 ggcgtcagtg cgcgcgggca ggcgggccgc ctgcaatgcc atgagcgcta cgagtgcatc
     5521 cacgcgcggg gcggccagcg atgccccggc cacaagttgt ccgagacgca acgcttccat
     5581 gcagatgtcg cgccgcatca attcttcgcc agagtgggcg gcgtatccag agctgaacat
     5641 cagatagatg acatgcagga cggaatcgag gcggtcgtgt aattccgagg gcatcgaaag
     5701 agggacctgc tgttcgcgca tctgcctttt ggcacgcacg aggcgctgcg cgatcgtgcg
     5761 ctcgtcggcc agaaagacgc gtgcaatctc gcccgcgctg aagccgccca cgagctgcag
     5821 gcccaacgca acacgggcgt tttcaggcag gtccggatga cagcacatga agaccatgcg
     5881 cagctcatca tcgcgcagtt gctcatccag ttccggatgg tccagactcg caggcccggc
     5941 tttgatgtcc tggacttccg acaactctcc ggattttcgg tcgcgccgca gaacggaaat
     6001 tgcataattg tgcgcgacgc gaaagagcca tgcttccggt ttttcaggaa caccatgaaa
     6061 agaccatgac tccaaggcgc gcaacattgc ttcctgtgcg gcatcttcag ccagttgcag
     6121 atgctgtggt ccgagcagtc cggcaagcca ggagatgatt ctcccggcct gcctgcggaa
     6181 caggtgctct acgatgaccg ggctgctttg ttccgtcatt tacttgtcag cggggcgggg
     6241 gacgacttca cggacttcaa tggtgccgaa gtctatatgc ggattcgtga gcgcgaattt
     6301 gactgcttca tcgaaatttg ccgcctcgat ggcatagtat ccgccgagca cctcacgtcc
     6361 ttcactgaaa ggaccatcgg aaacggagac gctgccgttt tgtttccgga taacacgtcc
     6421 ctggtgatcc agcctcgcgc cgcctaccgt gaacggcttc tgccgccatg caaaatactt
     6481 ctcggttgtc ctttgggcct cttccgggct gagccgggaa aaagggtctc catcatttcg
     6541 caataaaatc atgtactgtg gcattgtttt cctcctggga actcccttcc gggaattctt
     6601 ccatgccaat aacgcagaag caggcgggaa ctcgacagta ttttggaatt tttttacatt
     6661 accggagagg atgcaaccgg ggcatctggt ttatggatac ttgcggaccc atgaggcacc
     6721 cggctgaacc cacatcgctg agattcttcc tcccttcggt cgtcagaatg acggattcta
     6781 cgcagtcgtc agagtgacgg aactctgtgg ctaccaatag aagtgaagca gagagacccc
     6841 ctgacgaggg agattgatgt gcatacccgc atctctaagc cgcacctgct ttatcatctc
     6901 taattttcca gcctgctcca gttgctgctg ctgcgcaggc gagggatgct gtggactgcc
     6961 catcttctgc cagatggcgt aggcgttgct gtgatcccgg tccatacgaa attcctggac
     7021 gcgtactccc ttcgcgggca ggccgaatac agaaaccgat atctctgcgg gaggggcagt
     7081 cacgtcatca tcatggtagt tccagaggag cacgttcagc tcacggccat tttgtgtggc
     7141 gatggcgctg acgtccggtt gcgcagttac gctgtgttga agaacatctt cgacaggcac
     7201 ggcccctgtg ctttctaccc gcacccagtt tccacccagc ttgccgtaca tgcggaagac
     7261 gttgagtaca gccttatcta tgccattggt tgcaagctca cggaaacctg cgaaataggg
     7321 ctggtcttca aattcaaagg cccaggtgac ggatcccaga acggtcatgc cgtttttgcg
     7381 ggccagttcg taggtcctgg caatggcttc tgccacgctg acgccgtaaa gcggcccatt
     7441 tcgatatccg ttctggctgc ctttgcaggc ggcgcatccc tcggggtctg actcaccgag
     7501 gatgatggga gtgttcttcc attgcggata ggatgcaatt acgcgcatgc cgtaatctac
     7561 ggcgcgcagt tgttctccga tgttcatctg cacatggcta ttgatgaatt tcgggctgcc
     7621 tttggggtgg tagctgatga agtcgagcgg cgacccggtc tggcccgtgg catagttttt
     7681 gccattcgcg cagtgctcca gaaactgacg caggaacctt tcggaacgcc cgggatgaac
     7741 acctgttgct tccgggccgc cgattctggc gtcgggcaat accttacgaa tggccgcagc
     7801 agtgacatcg taaagcttgt cgtactcttc gggagtgccg tgccaatagg cgatgtcggg
     7861 ctcattccag acctcccaaa gccagccatg gacgtcattg ccatagcgct gtttcagatg
     7921 ctgaacgtat gaaacgacaa gattgctcca cttcgcataa tctttcggcg gatagctcca
     7981 gccggtgaag acgtcgccat tgggaaaatt gtgacggtaa ggttgaggat gcgtagagag
     8041 ggcctgtggc atgaagccga tttcgaccag cgggcgtatg tgcgccgtct ggaatgcgtc
     8101 gaagattttg tcagtgatgg tccagtcata tacgggcgtg ccgtcgggct tttctgtgta
     8161 gacgttggtg gacccccatt tgagggaacc ctggccattt cctgttgtga agagattatg
     8221 gacgcgaaag tagaccggca caggactgag cgcactcagc tcgttgagca gcttcaggcc
     8281 atttggcgca taaatgtaat tcggctcatc cgcgccaaag tagttccaga tgggaatgta
     8341 ggggccttcc gtctggtctg cgtgaacagt gatggtcacc tgcctgctct gtgcagacag
     8401 gtgaaatggg cacagggcgg cgatgaggag gagaaacgtt cgacgtgctc tcacctgcgc
     8461 agagtagcac aatgcggcgg tcgtgtattc cggatggccg ccgctccatg cttacgccag
     8521 cgcgagcgcc gcttctaccc cagcatggaa gccctgctgg gccaattcct gaccacggtt
     8581 ctggttctcg gtatggataa agtggatatc tttcagtccg agaaacccca ggatggcacg
     8641 caaataaggc tcctgctgat cgaaggcgcc tactggcgtt ccagcaggat aggccccgcc
     8701 ccgcgccgta atcacaatga ccttcttgtt tccgtccaga aggcccttgt aaccctctga
     8761 ggaatacgcg aaggtgcggc ccgcgcgcac aatctgatca atccaggttt tcagatgggc
     8821 cgagataccg aaattgtgca tcggaacagc aatgacaatg gtgtctgctg cttccagctc
     8881 gccgatgaga gtgtcagaga gcgccagcaa ctgcttttgt tccggcgtgc ggttttccgc
     8941 gggggtgaag actgcagcaa tccacgcttc tgtaatgtgc ggaacaggat tggcggccac
     9001 gttgcggcgg accacagttc cggtgggatt ctgttgctgc cattttgtta cgtactcacg
     9061 cgctacggcg ctggaaaccg aagtcgcgcg cggactagat tcaatgaaca ataaactcgc
     9121 catttttttt gctcctggaa gtgttttttg gggtggaaat aacgcctgtc catgctcaag
     9181 cccggcagaa atcgcgccgg attttttaaa tcatctgtta caacgaataa attgccgttg
     9241 caaccgatag gattcaacgg cgcccaggaa agatgcagga tttttgaggt ctatttaagc
     9301 tgccgggcga gcagaagttt ctcaaccgcg agccggtctt cttccgtatc tacgccgatg
     9361 gtgtcatagg gcgtggaagc cacataaata tcaattgcat tttcaagaaa acgtaattgt
     9421 tcgagctttt cgatctgttc cagcctgcgg gaagggagcg ttggaaaccg gtcgagcgcg
     9481 gccttgcgat aggcgtagat gccgaggtgc ttttcaaagg gcacatttcc ggtcttgtcg
     9541 cggtcgtatg gaatggcagc gcgcgaaaag tagagggcgc gtccgttggc cgctgtgacc
     9601 actttgacgg cattcgggtc ctggatatga ccaggagcgc agggggttga gatggttgct
     9661 acttctacct cgggccgttc catgagtttg agcaacgcgt ccagatgttc aggccgtaac
     9721 atcggctcgt cgccctgaat attcacatag atgtcggctg gaaccaactg cgcgacggcg
     9781 tagacacggt ccgatccgct ggcgcaaccg gcgggggtaa acatcgcggg gaagctgcgc
     9841 gaatgcgcga actccatcac ttcgtcggag tcggtggcaa tcaggacttc atccaaccgg
     9901 gggcaggcac gcgccgcgcg gtagacccag gccagcatag gctcacctgc aatttcacgc
     9961 agtaccttgc ggggcaggcg ggtggaagcg aggcgggccg ggaccacgcc gaggatgcgc
    10021 tgtttttctg ccaatagatg ctcctgttaa caggcctagt gtaattaaag accgctgtgt
    10081 tccacaggta taagcatgat tggaatgcag tttgacccgc tgcatcatcg tccgtatcat
    10141 aaggaatagc ttttcccacc ttaaaatggc ggcgtagctc agatggttag agcgtaggat
    10201 tcataaccct aaggtcggtg gttcgatccc acccgccgcc accagagaca tcatttattt
    10261 tccaagcgca agcgaggcca cggcagtcag ctccggcggg gcgaattctt gtgccaggaa
    10321 tttgggccag gcggcggcag caatcatggc ggcgttgtct gtcgaaagcg acagcgatgg
    10381 aaatccgatg ggcagaccgc ggtcggcggc ctcggcggtg aagcgtacgc gcagctcgcg
    10441 gttggcggcc accccgcccg agacgaggat gctggcggcg tcaaatgtct ctgcggcctg
    10501 aaaggtcttg cggcggaggt catctacaac ggcgcgctgg aaagacgcga tgaggtccag
    10561 tgtttcttta tcgcaaagcg agagggcatc tgccggtttt gcatggggga tacgcgccag
    10621 cgcggcgcgg cgtgcctcaa tggacgcgga catttcatgt gtctgcacat agcgcaggac
    10681 ggcggtcttg atgccgctga aagaaaagaa gaattgttgg ctgaactctt cgcgcagcgt
    10741 ttctttgcgg tgcactttgg gctttatctg ggcgaaagag aatgggacgg ccttagggtc
    10801 gccgtgcggg gcgagggcgt ccatccaggg gccgcccggg tagcccagac ctaataattt
    10861 ggctaccttg tcgaaggcct ctccggcggc gtcatctact gtatggccca cgtttttgta
    10921 gcgccagctt tctcgctgat ggcgggcaag gtagaggtgt gtgtgtccgc cggaaacgac
    10981 cagcgcgagc gcgggcaagg gcagtggttc ttcgcgtagt tgcgtttgtt cgagcagcac
    11041 cgcgtgaatg tgcccttcga ggtgattcac tgcaatcagc ggcttgttca gcgcgaaggc
    11101 gagtgacttg gcgtagctga tgcctaccag caatgctcca gccagccctg gcccttcggt
    11161 gacggcgatg gcgtctaatt cctgaagaga tgtgtttgct tcggccaatg ctgtgcgaac
    11221 gaccggcatg atgttgcgca ggtgctcgcg cgaggccagt tcggggacga ctccgccata
    11281 aggagcgtgg gtggcgatct gcgatgcaac aacgttggag agagtttcgc ggccttcgcg
    11341 cacaacggcg gcggcggttt cgtcgcagga gctttctatg ccgaggataa gaccggtgcg
    11401 catctttcta tgatagaaga tagtgatttc ctataccccg gccttgacaa tagccaacgc
    11461 cgtcatacac cggatgacaa acgaatttcc atgacaaaaa cacacgcaca actcgcggcg
    11521 gaacggattg tggagcggct tcgggcagaa gggtacgatg cgtactttgc cggagggtgt
    11581 gtgcgcgatc tgctgctggg gcatgctcca gcggactttg atgtggcgac gagcgcgcgt
    11641 cctgaaacgg tgctggggct ttttcggagg acgtttgctg taggagccca ctttggcgtg
    11701 gtgctggtgg ccgatgaggt ggatggcgag cagattgtta cggaagtggc cacgtttcgc
    11761 aatgatggaa gctactcgga tgggcggcgt ccggatgagg tgagcttttc agacagcgcg
    11821 caggaagatg tgctgcggcg cgactttact atcaacggaa tgctgcttga cccgaggatg
    11881 ctgactgaaa cgggcaacat ggaggcagcg gtactcgatt atgttggcgg gcgcgctgac
    11941 ctgtcggctg gcatggtccg cgctattggt gagccagagc ggcgcttcag ggaagacaag
    12001 ctgcggatgc tgcgagccgt tcgttttgct gcacggtttg aatttgccat cgagcaggtg
    12061 acggaaactg ccatttgccg tcatgcgggg gagatttcgc aggtgagcaa cgagcgggta
    12121 cgcgacgagc tgacgcgcat gttgacggaa gggcatgcac ggcgcgcatt tgaattgctc
    12181 gaccagaccg gattgctgaa gcaggtgctg ccggagattg accgtctgca tggagtggag
    12241 cagccgccgg agtatcatcc cgaaggcgat gtgtgggcgc atacgctgct gttgctggaa
    12301 aaactgccag cgggagtatc gcctacactg gcgtggggcg cgctgctgca tgatgtgggc
    12361 aagccggcga catttgagcg cgcaccggac aggatccgct tcaacggaca tgtggaggtt
    12421 ggttcgcgga ttgcggcgga gatctgccgc aggctacgct tctcgaacga gcaaacggcg
    12481 cagattctgt cgctggttga aaaccatatg cgtttcggcg atgtgatgcg gatgaaggaa
    12541 tctacactga agcggttctt ccggctgccg aagttttctg agcatctgga actgcaccat
    12601 atggattgcc tttcgagcca cggcgatctg acgctgtaca actttgcgaa acagcggtac
    12661 gagacgatgc cggaagaggt ggtcaaaccc cggctgctgg tcaccggggc cgatctgatt
    12721 gcggttggct acaagcccgg tccgcggttc aaggagatgc tgtcgctggc cgaagatgcg
    12781 cagctggaag ggcatatccg gacgaaggaa gaagggttga agttggtgcg ggacacattt
    12841 ggcgggctgg cggctgatcc tgaagatgaa agacttcgtc atcctcgagg agcttaagcg
    12901 agcttcgatg ggtgagtccg cggagatatt cgttcccctc gtatcgctcc ggggtctaag
    12961 aatgacagcc cggactttta acgaagcggc tattagcgaa atggtaaatt attgaaaata
    13021 aagggaaatt tttgtctgtg aaaaacgttc ttgacaattg aattagcttt cgaatacctt
    13081 ttctctccgt gggccaataa aacgatttat tccgaacaga cgattttaat caagcgatta
    13141 agcagagctg ttggcgatga aaccgttttc cacaagggga ttgtgaatct ccctagatgc
    13201 agtttgaaat gaagtgcttt ttacacggag gcaggcaatg aaacagacga cagccggtat
    13261 ggcgtcgaga gatctcggtg ccctctcgag gcatgttcca tttaccaggt tatgttggtc
    13321 gcttgcggga aggacgattg ctgttctggt atgcatcgtg ttcctgtttg cgtctgcgat
    13381 gcgcgggcag ttgacgacgg ggagcctttc cggaacggtc gctgaccaga gcggcgcggt
    13441 tgtaccgaat gcgcgggtga cgctgacgaa tgaagcttca cacgacacgc gcctgaccac
    13501 aagcaacagc gcaggatatt tcagctttgc agcggtgcag cctggcagtt acacggtcac
    13561 gattgacgcc aacggattta acatctggaa gcagtcgggc attcccatga acccgggcga
    13621 tatccgggag gtcagcggca tcaagctgac gacgggcggc gggacgacca cagtcgaagt
    13681 ccaggctgcg gcggcacaga ttctcccgac agattctggt gagcgggccg cagttctgag
    13741 cagcaaagac attgagaagc tggcgctcga gggccgcaat gtctctgagc tgctgaaagt
    13801 gcttccaggt gtttcgatga cgtcgggtgg aaccagtggc ggtacctcca tggacttttc
    13861 cgcgtccgcg ccgacgggca gtacggtggg gaatggactg acgacgagcg gcgcgccata
    13921 ccgtggcggc acggcttata tgctggatgg cgcgaacatc attgatcctg gctgcaactg
    13981 ctactcgatc gcggtcccaa atcctgacat gacgcaggaa gtgaaagtgc aaggatcatt
    14041 cggcgctgac agtccgaatg ggccggagat tgtaaacgtc atcagcaaga gcggcggatc
    14101 gcaatatcat ggcgaagggt acttctatgc ccgcaatcag gtgctgaacg ccaacacgtg
    14161 gctgaataac aacagcggca tcaagcgtcc gaatggagaa tactactatc cgggcgggaa
    14221 cctgggtggt ccggtgaaga tacccgggac gagctttaat gccaacaaca agctgctgtt
    14281 ctgggtgggg tatgaatact ataagcagac acttcccgcg gcgcagccgt ttgaatcata
    14341 tattcccagc gcgggcatga tggcgggaga ctttactccc accggtgacg gcaatgctgc
    14401 gttgtgcccg aacggattca gctccagcgc aaacaactgg tgcaacgacc tgagtggcgc
    14461 agtggccccg gacggtactc cgattacagg cggtgtgatt cccgctaagt atcttgatgc
    14521 gggtgcggcg gcgctgatga agatgtttcc gaagccgaat gcaaatccag ccacgactcc
    14581 gggcggcttt aattacatcc agcccatcac gaacgagcag aacggttatg tgttccgcgc
    14641 gcgcgtggat tacaacctga acgacaatac gaagttcttt gcgacctatc agcagggcaa
    14701 tacaacgttc attactccgg gccacgtgtg gtggacgcct tcctacacga ttccttatcc
    14761 gggcggaaac attacgagtc cgaatacttc acgtgtggtc acagcaaacc tggtgaatat
    14821 catcacgcct acgcttacga atgagtttgt gtttggctgg ggatgggatg atggtccgtt
    14881 caatccgtca aagctgagcg cgatatacaa gtcggcgctt ggatatccgt atgcgacggt
    14941 gtacaacacg gcatcgctgg ttgcgccttc gattaacagc gcaggcgcgc agactttccc
    15001 tgatatctca cagcctgcat tctttggaac atcaggaagc tatccgctgt tgaaggccat
    15061 tccctcgttt gcagataacg tgacgaaggt gtggcgctcg cacactttca agtttggagc
    15121 gttcacggag ctggtaggga accggcaggg tgtgtggtcg tatcccaatg gcgttctgtc
    15181 tttcggaagt tcgcctgaat ccaacaacgt ggccggttcg caggtggctt ccggcacgaa
    15241 gattggttca tccaacccaa cggcgaacct ggtgatgggg attgccagcg gattcagcca
    15301 gaacaattac gtgccggtgc aggacatggc gtaccggacg actgcgggct acatcatgga
    15361 caactggcat gtgaattcca ggctgaacct gaacctgggt gtgcggtttg accatattgg
    15421 gcgttggtat gaccgccagg gcactggtct tgcagtatgg ctgccgcagc tttatcagca
    15481 ggacgttgcg gccaaccaga ccgcaggcag catggtggtg gagtatcc
//
LOCUS       DASRBV010000023        21403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_558168, whole genome shotgun sequence.
ACCESSION   DASRBV010000023 DASRBV010000000
VERSION     DASRBV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 21403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21403
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558168"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..618)
                     /locus_tag="VFA02_01000"
     CDS             complement(52..618)
                     /locus_tag="VFA02_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU08451.1"
                     /translation="MKAIRFFYIALLCCLMTPATLTYAHAQAATAQASPAQTFNNLMS
                     ALESEVVGAADAMPADKYNFAPTQGEFKGVRTFAQQVKHLTEANYGFFQGWNVPGAVK
                     REDIEKLTSKDDIMKALRDSYAYAHKALDTLTPANAFEEISFGQRKSTRAGIAAFCIA
                     HSMDHYGQMVVYLRMNGIVPPASRRNGM"
     gene            805..1965
                     /locus_tag="VFA02_01005"
     CDS             805..1965
                     /locus_tag="VFA02_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZU08452.1"
                     /translation="MKRVYMDANATTPLLPEVLDAMRPYFIEHFGNASSIHQQGQQAR
                     AAVEHARESVAALINCRPAEVVFTSGGTESDNMALFGLVQQGEHLITSSIEHHAVLHA
                     AEKLRDRGMEVTFLPVSSEGVVDPQDVRRAIRPNTKLISIMMANNETGAIQPVSEIGR
                     IAAEADVYFHIDAVQAAGKLPVDVQTLRCDLLSISGHKMHAPQGTGILYVRRGTQLEP
                     LFFGGAHERQRRAGTENLPGIVGLGKAAEIAQTALTDGTIERIRTLRDHLEQGILAQV
                     EETGVNSRNVARVANTTNIYFDSLEGEALVIALDLKGLCTSGGSACASGATEPSHVLT
                     AMGLPPARARASLRFSLSKLNTEDDIAFALEIVPAAAARLRQLAPVNAGTLG"
     gene            complement(1978..2427)
                     /locus_tag="VFA02_01010"
     CDS             complement(1978..2427)
                     /locus_tag="VFA02_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08453.1"
                     /translation="MKSTIAIIVLTVTTTAFAEKQIRKSEMPIAVQKTADQQSAGATV
                     RGYAKDVENGRVEYEVELLVNGHTKDVTMDPLGNVTEIEEQVEMTSLPASVSSALRKR
                     ANAGKITKVESLTKQGRLVAYEAQVVKNGRHSEIQVGPEGETLDHEE"
     gene            2530..3648
                     /gene="mnmA"
                     /locus_tag="VFA02_01015"
     CDS             2530..3648
                     /gene="mnmA"
                     /locus_tag="VFA02_01015"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZU08454.1"
                     /translation="MTGTFNNTVAVAMSGGVDSSTVAAMLRDEGYELVGLTLQLWNQR
                     RLAGREGMPEQVQGRCCSIDDVYDARRVAEALGIPYYLINEQERFERDVVRPFVSEYL
                     AGRTPIPCSLCNNHLKFDQLLVRARQIGADRIATGHYARNEYDPVRNRWILKRPADLS
                     KDQTYFLFGLTQEQLSRTLFPLGGYTKPEVREIARQHQLAIAVKPDSQEICFIPGGDY
                     KRFIDAYLDEQGEQMPNTSGELVSTSGEVIGHHQGIHNFTVGQRKGLGISSPSPLYVI
                     QIDSASHKVTVGHESETTINTLRARDLNWISIPSLDAPMRVKAKIRHRHEPAWATIEM
                     SGPDEVLATFDEPQRAVTPGQAAVFYDGDEVVGGGWIV"
     gene            3748..3966
                     /locus_tag="VFA02_01020"
     CDS             3748..3966
                     /locus_tag="VFA02_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08455.1"
                     /translation="MPFPIKTCVVCGEEFELKPDKPGFANRCPECSQPEEDSTGATTN
                     MVGGRKEASELNEARRKAMRDLLYRKDV"
     gene            4074..5153
                     /locus_tag="VFA02_01025"
     CDS             4074..5153
                     /locus_tag="VFA02_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU08456.1"
                     /translation="MPKQKSKASKEKSPFLPGRPISLKVLGDYLDLSPATISLVLNNA
                     PGVRSIPQETRDRVLAAAKKFDYRPSFYARSLRRKQTFSVGVLVPELSDGYSVLVMDG
                     IEEVLLEEGYFYLTASHRRKADLIEEYPRLLMDRAVEGFVAIDTALQHPLPLPVVAVA
                     NHKRIEGVTSIVLDHHRAAELAMHHLYQLGHRHIAFMRGQTFSSDSDDRWKSFTSVSR
                     KLGIEVHPELTVQLEVNVSSPELGYPVVHKLLAQKRPFTALVSFNDIAAIGAIRAFRD
                     HGLRVPEDVSIVGFDDIQGAAYHNPSLTTIRQPLHAMGTTAARMLLQRIRGEKEVPDQ
                     IAIVPELIIRESTCPPNAKRLSRRP"
     gene            5406..8858
                     /locus_tag="VFA02_01030"
     CDS             5406..8858
                     /locus_tag="VFA02_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08457.1"
                     /translation="MNFIPKKLLILAVTLIVVGVSSYAQLDTGSVNGTVTDQSGAVVA
                     GATVLLRNTQTNATRTITTTASGTYVASGLSTGSYQITVNAPGFQPFSGQVEVTVGGN
                     ATVDAKLNVTQATTEIQVVGEGGTQVNTESQELSQIVNTQQMAQLPSLTRNPYDFVSI
                     AGNVSNGDNTTPSGSQNLSSRGVSFSINGQRESGTEILLDGVENIDLFGANVGQQIPV
                     DAVQEYRVVTNNFSAEYGRASGGVVNVVSKAGTNSLHGSAWEFNRLSAYTANTYDNNA
                     LGQPKGAYTRNQFGYDIGGPVLKNKMFFFQSTEWTRVRSQPTESQLVPTPQFLAYTAP
                     NVQAYYSAYGATPYPITTTYSQSDLNISLPGVPSSTPILGQVNYKANADAGGDVPQNT
                     YSLVGRLDFNPTDRTQMYFRFAQQSIDQFAGATFYSAYPQYDIGQGTYNNSGIFSFTH
                     AFSPSVLSNTKASLSRLNTSNTYNKATQNVPMLMFTSNTANGFSIAFPGLQNLGQPGV
                     GGYPFGGPQNTLQAQEDLSWMKGGHSLRFGGQYTYIQLNKAYGAYAQAVEVLGNGLQS
                     GLNAMMAGTLQYYEAAVNPQGKYPCITNPDGSQTVTPSCEVNTPVTSPSFSRSYRYHD
                     WALYAQDSYKATPRLTLNYGVRYEHYGVQHNDNQNLDSNFYPAAGGNYYQSIRNGQVY
                     LAPQSPIGSLWAPSWGTVAPRVGFAYDVFGNGSTSLRGGFGISYERNFGNVTFNVIQN
                     VPNYATLQIFNTPVTTSNLGPLASPGVQPLPSTELRMPDPHIKTAQTQFWSLALERQV
                     LRNSVLALEYSGAHGVHLYDITVSNPIGGGQFYLGDPLSSAASCPYTNPATGAGACYT
                     RPNYQYAGINTRGSRGSSAYEALNVRFQTQDLHNTGLSMTANYTYAHSLDDLSSTFAD
                     SAQGGSGYIGNLGYLDPTNPGLDWGSSDFDIRNRFVVAPIWQTPWFKNGKGFARQALG
                     GWTLVGIFTTRGGTPFSAFDYSYNQNGYFGVSRLVPASPVTQYKTGKGVAVGPNQFQV
                     LSLPANNPFFNPALGLSDFGPFPSNMTRRNAFRGPGAWNTDLAASKSFALTERIGLLL
                     RAEGFDIFNHHNLYVNESALADFAPGSPALPVIALKGGLNTIALGGNHDERRFGQFSL
                     RVTF"
     gene            complement(9004..9183)
                     /locus_tag="VFA02_01035"
     CDS             complement(9004..9183)
                     /locus_tag="VFA02_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08458.1"
                     /translation="MAKIAKTADRKKVMDTSKSTDCPKCSKPTRIVKRVKDRERGIPG
                     GVYISCSACDFYEKL"
     gene            9444..10823
                     /locus_tag="VFA02_01040"
     CDS             9444..10823
                     /locus_tag="VFA02_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU08459.1"
                     /translation="MIVEDEENALTGLAELVSGWGYRTETARDGLEGLERAQAWQPGI
                     VITDLKMPRMDGLELLERLSELKQDNLAVIVLTAQGSIEQAVEAMKMGAYDFIQKPID
                     ANRLRTILGNASRQRETQLELEVTRRKLRETGVLGHLVGSSKKMQELFALIERIASNN
                     VSVLITGESGTGKELVARTLHDLSPRKAKPFVAVNCAAIPETLIESEIFGHEKGAFTG
                     ALERRAGCFELAEEGTLLLDEIGEMPIGTQAKLLRVLEDRKLRRLGSKVESPVDVRVL
                     AATNKDPETAVADGHLRGDLYYRLNVFNIHMPPLREHKEDIPEIVDSMLKDMNEKHGR
                     QVAGITDDMMARLMAYDWPGNVRELRNTIERAVILCGNGYLEPKHLPPGFGSKPAKHV
                     PESVGNIVQVEVGTTVDEAERRLIMKTLESTNNNKTRAAEILGISLKTLHNKLKEYGS
                     ASGSQEAEE"
     gene            10871..12811
                     /locus_tag="VFA02_01045"
     CDS             10871..12811
                     /locus_tag="VFA02_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU08460.1"
                     /translation="MRLKTKLVFAITGLVFVVVLVFSWLYFSQLLQQHIDQSYNATDI
                     VAHQLLFATRQAIENGTRNQQIDPNDPVALRAAVASALRNDAALNALLNSVISYSPTV
                     FDISIADQNGRVYLSTDPILQDQFVPQRVAYASLRKASLVQILEVVFGPPRVYDVSLS
                     LERNNQPFVTVQIGIRTTFLSHAFRPWFFEAASLIGFAVVITLLVSAMVANLAFKPIE
                     LISKRLDLLTAEVQSQENDPVVELENPDGDDTVLQVSHKIERLGRRMKNVEEVFSSLK
                     QNLDQILSNLQDGMILFTRDARAVLVSSSVERFLARNRDEILGASAHDIFDRSTILGR
                     VVREAFDRGIGLVQEEITTETGRRVEVSLDFIHDDRVPQQRDGLGALLTLHDLESVQE
                     IESELEISRRMAAIGQLTSGVGHEVKNPINAIVVHLELLRTKMTAPDPSATRHLDVIQ
                     NEIQRLDRVVQTLVDFSRPVELKLHEQDLRPVVNAVLALASVELETRNVRVLNSMPNH
                     PVIAKIDADLMKQAILNIVLNGAQAMVRGGILRVNLAEDGRWSYLSISDEGEGISPDV
                     REKIFNLYFTTKKEGSGIGLAMTYRIVQMHNGQIDVESEVGKGTTFTLRVPAFSTSEN
                     RLRGHLEAEAAKSASFVKEPWG"
     gene            12808..13413
                     /locus_tag="VFA02_01050"
     CDS             12808..13413
                     /locus_tag="VFA02_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08461.1"
                     /translation="MNTAFRTAVIVSACLLPLAGCSHRNKVLPPQQAQAPVPPPSMMV
                     HYPALPPLPPPSAPRVELASVQETPPAPRPHRASHRKPSPAKTAPGTETAAEKQPQQQ
                     TTEVASAAPAPDASPIGQLTAAGDATNIQQRRDIEQLITNTENGLDKISRNLNADEQK
                     TAVQIRTFLTKARQALTDNDLDGANTLATKAKVLLNELTKK"
     gene            complement(13438..14157)
                     /locus_tag="VFA02_01055"
     CDS             complement(13438..14157)
                     /locus_tag="VFA02_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZU08462.1"
                     /translation="MRKKLAVCFAVVASSLFSLQMVGQGKPAAANSSPASAALATRPW
                     EFGPFFQGGVGVGDRSDFTFASAGMRLGKVMTDEHLPGILRGQFEYAGEFMPYWQSFT
                     PAPHLQWTKYSFEGQTGEALLPYNGGTFTGVSITPIILRWNFKPGRRFAPWFQGAGGL
                     IWTNHKYPPDIIVQHGDAGGTSVWNFTPQAGIGFHYFVKDRQSLTFGVNAVHISSASL
                     GDRNPGVNASMQFQIGYTWWK"
     gene            complement(14244..14990)
                     /locus_tag="VFA02_01060"
     CDS             complement(14244..14990)
                     /locus_tag="VFA02_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU08463.1"
                     /translation="MSGHSKWHSIKHKKGALDAKRGKIFTRLIKEITIAARAGGDPDG
                     NPRLRTAIAAAKAENMPADNIKRAIQRGTGELPGATYEEIAFEGYGPGGVAIIVETTT
                     DNRNRTVSEIRHLFSKHGGNLGETGSVRFMFTKKGIIAVPKTAADEEKLMSIVLEAGG
                     EDLNDEGDTWEIITEPQNFEAVAQAVRDAGIETVMSEVTMIASTYTKLEGAAASQMVR
                     LLEALEDHDDVQNVYSNFDMDAKQLEEVAG"
     gene            15227..16642
                     /locus_tag="VFA02_01065"
     CDS             15227..16642
                     /locus_tag="VFA02_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU08464.1"
                     /translation="MKLFRRALFFLSAPCLLPLHPAWGQTTSRAEEIRELRQQLANLN
                     ARLTKLENEESAGTTPAGDPTANAAALSAASSALTAQPEPESTQSKQLTSADRSTLDS
                     LNGTTFTMGLDGYYEYNFNDPIGRANLLRVYDVSSNAFSLSQANFIIEHAPHPEHGQR
                     FGARLDVQYGQATQSLQGNPSNEPRSSIYRNIFQAYGTYVAPVGSGLTMDFGKWASSV
                     GIEGTYTKDQMNYSRSIWFGFLPSYHMGVRLNYGFTPWLSLRYATVNGIQQTEAFNGF
                     KDQMFDLDIKPRKSVDWNINYYLGQEHPDFQYVTNGPGTLPTQQGMPFEPIPHPAKGK
                     IHIFDSYATWSATQKLTLALEGDYAISRDQTWSAPQHVDGGAAYARYQFNPQFALAAR
                     AEYFADPNALYTGKSQAIKEFTLTFDHALTNGFLMREEFRHDFSNQPYFLTSTLGRLN
                     HDQTTATLGLLWWFGGKQGAW"
     gene            16787..20092
                     /locus_tag="VFA02_01070"
     CDS             16787..20092
                     /locus_tag="VFA02_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08465.1"
                     /translation="MLRNKTFILASALFVALLIALCPVLLLSQAVSVASVAGRVTDPQ
                     GAAVSGAEVKMTSTETGAVRSVITNSEGLYSIPNLPIGGYTLEASAPGFQTYVQKEIS
                     LRVNDNIQINVSLSVGNVSQQVTVSAAAQMVQTQDNSISQVIDQHRIAELPLNGRQAT
                     QLILISGAAVTTPGGDMTGSKNFYSSTTISVAGGQANQLNYLLDGGDNNDTFSNVNLP
                     FPFPDALREFSVETNALPARNGLHPGGVVNIVTQSGSNQFHGVVFEFLRNNVLNATNF
                     FSTKRDTLKRNQFGGTFGGPVIHDKFFFFGGYQGTRNRQTPNSATAYVPSQAALNGDW
                     SSMESAGCQSSGKARVIKNPYTGQTFANNQVPTSLYNPAALALLQYLPVSSDPCGKVV
                     YGIPTTGDEDQGILRLDYTFSPRQQLYGRYFIVDYRNPAVFDSKNILVTTNPGNLERA
                     QAFTLGDTWTISPNLVNSAHATITRRRDDRGPAPNMINGGKLGINTYFYVPNDLRLSV
                     SNGFNTSCGTCAPGFFNVTTGQAADDVDFLKGRHQFAFGADFIRTDNNLETGYLENGY
                     YQFNGQYSNDPLLDFMLGAMNGYGQSRPQQVAMRESIIGVYGQDTIHVNPHLVVNIGL
                     RWEPQLFPHDYFHRGSTFSQAAFNAGQKSTIFANAPAGSFYYGDKGVPAAFTKNTLTN
                     FSPRFGLVFDPRGDGRQTFRAGFGLLYDSGMLYATQRLTSNPPFVNEIDLTGANGPGG
                     SIPFNQPWSAYGNIPFPAPYPPPSDVSFPQFPLFVVLPDRVKPTYIEQWNASYQLGFA
                     NNWLFSMSYLGNHTMHVWGGLPINPAVYIPGNSTTKNTNQRRVLYLANPSQGQYYGSI
                     FLLRDEGVANYNGMLVSVEHRMSSNFTVLANYTWSKCLSDTDFDGDIRQSNYQNPANP
                     RADYSVCNFDVPNLFNLTAVAISHFSGGWKQKILGNWQIAPLIRISSGMPANITSGQD
                     YSLTGTGMDRPNRIGNPYLSHRTCTQAACQWLNPASFAKNAAGTFGNMPRNGVRTPTY
                     ADVDAALSRIFPITERWQIEGRFESFNLLNHPNFGNTAGNLSTNLNSSSTFGRITAAG
                     DPRIMQAALKIRF"
     gene            20261..21361
                     /locus_tag="VFA02_01075"
     CDS             20261..21361
                     /locus_tag="VFA02_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08466.1"
                     /translation="MKILKYAVLALGMLFPLSAFADFQYQETTQITGGSLMNMLKMAS
                     VFSSRARDQMNAPVVSAVYIQGNRMARINQYTMEIIDLDKETITNVDLQKHTYTQMTF
                     QQMREAMEREMQKAREQQAKQSGDQSQAANNVDIQFKINVRNTGATKQLGGLETSESI
                     LTMQMVGTDKTNGQQGAFAMTNDMWLTPSVPGYEEVREFYKKYALKMGQTFNGSNISQ
                     SLMAMHPGMSQGMSDMAKEVEKMKGIPMLQVLRIGATANGDPLPAASEAPLPASSGPQ
                     TPSAGEIAQRSAASAIASRLGGFGLGGFGHKKQQQQDGSANNAQANAAQQQTASVLME
                     MQTQMDSFSSAPIDASKFAVPAGFKQIEPQMH"
BASE COUNT         4907 a         6526 c         5406 g         4564 t
ORIGIN      
        1 ggcttgtatg gggtagtttg actaaatcgc aaccagaaac agggcttcag cctacatccc
       61 gttccgccgg ctcgccggag gcacaatccc attcatgcgc aggtacacca ccatctgccc
      121 atagtggtcc atcgaatgcg cgatgcagaa cgcggcaatg ccggcacgtg tgctcttgcg
      181 ctggccaaag ctgatttctt caaacgcatt tgccggagtg agcgtatcca gtgctttgtg
      241 cgcgtaggca taggaatcgc gcagggcctt catgatatcg tctttgctgg tcagcttctc
      301 aatgtcttcc cgctttaccg ctccgggaac gttccacccc tggaagaacc cgtaattcgc
      361 ctccgttagg tgcttcacct gctgggcaaa ggtgcgcacc cctttgaatt cgccctgtgt
      421 gggagcaaag ttatacttgt ccgcgggcat ggcgtccgct gcccctacca cctcactctc
      481 caacgcgctc atcagattgt taaatgtttg tgcaggagat gcctgtgccg ttgcggcctg
      541 cgcatgtgca taggtcaacg ttgccggggt catcagacaa caaagcaacg caatataaaa
      601 gaaccggatc gctttcacgt cgttcctcct ctttaaaatg actgcgcaaa aagcctagca
      661 gaagcggtat acgatgtgcg tcaaatcttc gttgaaccga ggcaaatata cgcctgaacg
      721 atagcatgga tgatccatat ctcgcagaga tgtgggttca cctgaaaacc acgtaaaata
      781 ctttcaggga gacttccacc atcaatgaaa cgggtataca tggacgcgaa cgcaacgaca
      841 cctctgctcc ccgaggtact cgacgccatg cgcccgtact tcattgaaca ctttggcaac
      901 gcctcttcca tccaccagca gggtcagcag gcgcgcgctg ccgtcgagca cgcgcgggaa
      961 tccgttgccg ccctcatcaa ctgccgtccc gcggaggtcg tcttcacatc cggcggcacg
     1021 gagagcgaca acatggccct ttttgggctg gtgcagcagg gcgaacacct catcacgtct
     1081 tccatcgagc accacgccgt cctgcacgct gccgaaaagc tgcgcgatcg cggcatggaa
     1141 gtcaccttcc tgcctgtgtc cagcgaaggt gtcgtagacc cgcaggatgt ccgccgcgcc
     1201 atccgtccaa atacaaaact catcagcatt atgatggcca acaacgaaac cggcgccatc
     1261 cagcctgtaa gcgaaatcgg acgcatcgcc gctgaagccg acgtttattt ccacatcgac
     1321 gcagtgcagg ccgcaggcaa gctgcccgtt gatgttcaga ccttgcgctg cgatctgctc
     1381 agcatctcag gacacaagat gcacgccccg cagggtacgg gaatcctgta cgtgcgtcgc
     1441 ggcacacaac ttgagccgct cttctttgga ggagcccacg aacgccagcg ccgcgccggt
     1501 acagaaaacc tccccggcat cgttggtctc ggcaaggcag cagaaatcgc tcagaccgca
     1561 ctcaccgacg gcaccatcga acgcatccgg acattgcgcg accacctcga gcaaggcatc
     1621 cttgcgcagg tagaagaaac aggcgtaaac agccgcaacg tcgcccgcgt cgccaacacc
     1681 acgaatatct acttcgacag cctcgaaggt gaagccctcg taatcgcgct tgacctgaag
     1741 ggcctctgca cctccggcgg ctcggcctgc gcttcggggg ccaccgaacc ttcacatgtc
     1801 cttaccgcca tggggttgcc tccggcgcgt gcgcgcgcca gcctgcgctt ctcgctcagc
     1861 aagctgaata cggaagacga cattgcattt gcgctcgaaa ttgttccggc cgcggcagca
     1921 cggttacgcc aactggcacc agtcaatgcc ggaacactcg gctaatcgcg gacttcctta
     1981 ctcctcgtgg tccagcgttt ctccctcggg tccaacctga atttcagagt gtctgccgtt
     2041 tttgacgacc tgggcttcat aagcaaccag tcttccctgc ttcgtcagtg attcaacctt
     2101 tgtgatcttc cctgcatttg cgcgcttcct gagagcactg ctcacgctgg cgggtaatga
     2161 cgtcatctcc acctgttctt ctatttccgt cacatttccc agcgggtcca tcgtgacatc
     2221 tttcgtatgt ccatttacca gtagctccac ctcatactcg accctgccgt tctcaacatc
     2281 ttttgcataa ccacggaccg tggctcccgc gctctgctgg tctgctgttt tctgtaccgc
     2341 tatcggcatc tccgatttcc tgatctgctt ttccgcgaaa gccgtggtgg tgaccgtcaa
     2401 aacaatgata gcgatggttg atttcattcc aggtttctcc tcgtgccatc aatgtacaaa
     2461 tgtaacctga agcagtcctg aaaaagacct gaagcgcagc ttaatgcgat actggaagaa
     2521 ggcgggaata tgaccggcac gtttaacaac acggtcgctg tagccatgtc cggcggagtg
     2581 gactcttcca ccgttgccgc catgctccgc gacgagggtt acgaactggt cggtctgact
     2641 ctccagctct ggaaccagcg ccgtcttgcc gggcgcgagg gcatgcctga acaggtgcag
     2701 ggtcgctgct gctccattga cgatgtctat gatgcgcgcc gcgtcgccga agctctgggc
     2761 attccctact acctcatcaa cgagcaggag cgctttgagc gtgacgtggt ccggcccttt
     2821 gtctctgagt acctcgccgg acgcacgccc attccctgct cactctgcaa taaccacctc
     2881 aagttcgacc agcttctcgt ccgcgcgcgc cagattggcg ccgaccgcat cgccactggc
     2941 cactatgcgc gcaacgagta tgatcccgtc cgcaaccgtt ggattctgaa gcgccccgct
     3001 gacctgagca aggaccagac ctattttctc tttggcctta cccaggagca gctctcgcgt
     3061 acactgttcc cgctcggcgg ctacaccaag cctgaagtcc gcgaaatcgc gcgtcagcac
     3121 cagctcgcca tcgccgtaaa gcccgattcg caggagatat gcttcatccc gggcggcgac
     3181 tacaagcgct tcattgacgc ttatctcgat gagcagggcg agcagatgcc caatacttcg
     3241 ggcgagcttg tctccacctc cggcgaagtc ataggccatc atcagggcat ccacaacttc
     3301 accgtaggcc agcgcaaggg actcggaatc tcatcgccct cgccgctcta cgtcattcag
     3361 attgacagcg cctcgcacaa ggtgaccgtg ggacatgagt ctgaaaccac catcaacacg
     3421 ctccgcgccc gcgacctgaa ctggatttcc attccctcac tcgatgcgcc catgcgcgtc
     3481 aaggccaaga tccgtcaccg ccatgagccg gcctgggcca ccatcgaaat gtcaggacct
     3541 gacgaagtcc ttgccacttt cgacgaacca cagcgcgccg tcacccccgg acaggccgcc
     3601 gtcttctatg acggagatga agtggtcggc ggcggctgga ttgtataaac ctgctctctt
     3661 cggggaaact ttgcagccct cccatgctgc aatccgcgca tggatttttt aaaataatga
     3721 aaagcaattg attcaacgtg gatagaaatg ccgttcccca ttaaaacttg tgttgtttgc
     3781 ggagaagaat ttgaattgaa gccggacaag cccggctttg ccaatcgctg ccccgaatgc
     3841 agccagccgg aagaagactc caccggagca accacgaaca tggttggagg ccgcaaagag
     3901 gccagcgaac tgaacgaggc ccgccgcaag gcaatgcgtg accttctcta ccgcaaagac
     3961 gtatagctcc tgtgaaaaac ccgtgccctc aggacgctgg acattaagcc cagcttcaca
     4021 tcatttccgc tttcctgttg aacatttcgt cagcaaacgt tacaaaagct gatatgccaa
     4081 agcagaagtc taaggccagc aaggaaaaga gcccttttct tcctggccgg cctatcagtc
     4141 tgaaagtcct tggcgactat cttgatcttt cccccgcaac tatttcactg gtgctgaaca
     4201 atgcccccgg cgtgcgctcc attccgcagg agacccgcga tcgcgtcctc gcagccgcca
     4261 aaaaattcga ctaccgcccc agcttctacg cgcgctccct ccgccgcaaa cagacgttct
     4321 ccgtcggcgt actcgtgccg gaactgagcg atggctactc cgttctggtc atggatggca
     4381 tcgaagaggt cctgcttgaa gaggggtact tctatctcac cgccagccat cgccgcaaag
     4441 ctgaccttat tgaggaatac ccgcgcttgc tgatggaccg cgccgtggag ggctttgtcg
     4501 ccattgatac ggccttgcag cacccgcttc cgttgcccgt ggtcgccgta gcgaaccaca
     4561 aaaggattga gggcgtcacc agcatcgtgc tcgaccacca ccgcgccgcc gaactggcca
     4621 tgcatcacct ctatcagctt ggacatcgcc acattgcctt catgcgcggg cagacattca
     4681 gttccgactc cgacgaccgc tggaagagct tcacctccgt ttcaagaaaa cttggcatcg
     4741 aggtccatcc agaactcacg gtgcaactgg aggtgaatgt ctcgtccccc gagcttggct
     4801 atccggttgt acataagctc ctggcccaga agcgcccctt cacggcattg gtttctttca
     4861 atgacatcgc cgccatcggc gccatccgtg ccttccgcga tcacggtctg cgcgtccctg
     4921 aagacgtctc aattgtcggc tttgatgata ttcagggtgc cgcctatcac aaccccagcc
     4981 tgaccaccat ccgccagcct ctgcacgcca tggggaccac ggctgcccgc atgttgctgc
     5041 aacgcatccg cggggaaaaa gaagtccccg accagatcgc catcgtaccc gaactcatca
     5101 tccgcgaatc cacctgcccg cctaacgcaa agcgtctgag cagaaggccg tagaaaaaaa
     5161 acgatcccag gccctcgctc tgataacttt aaagtaaatg attattaact gtttgataaa
     5221 cagtagttac agttttctga atttggacat tccaaaataa aaataattcc ttatatgtta
     5281 ccttgtttca aaatatatac gcgcagctat atgctgaaaa atgaataact aaattattac
     5341 tatccggcat acctggaatg gcagaaaaat tgcccttccc tatttctgaa gaaagggaaa
     5401 atggcatgaa ttttattccg aaaaagctgc tgattctggc tgtgaccctg atcgtagttg
     5461 gtgtatccag ctacgcccaa cttgatactg gttccgtcaa tgggaccgtc acggaccagt
     5521 caggggccgt ggtggccgga gcgacagttc tgcttcgcaa tacacaaacc aacgccactc
     5581 gcacgatcac cacgaccgca agcggcacgt atgttgcctc tggattgagc acaggttctt
     5641 accagatcac agtaaacgcg cctggatttc agccattttc cggccaggtg gaagttaccg
     5701 tcggcggcaa tgccacggta gacgccaaac tgaatgtgac ccaggcgacc actgagattc
     5761 aagtcgtagg agaaggcggc actcaggtca atacagaaag ccaggaactt tcccagatcg
     5821 ttaacactca gcagatggcg cagctcccca gcctgacccg caatccctat gattttgttt
     5881 ccattgcagg aaatgtcagc aatggtgaca acaccacacc cagcggctcg cagaacctca
     5941 gcagccgcgg tgtcagcttt tctatcaatg gtcagcgtga atccggcact gagattctgc
     6001 tcgatggagt tgaaaatatt gatctgtttg gcgccaacgt tggccaacag attccggtag
     6061 acgctgtcca ggaataccgt gtcgtcacca acaacttcag cgcggagtat ggccgcgcat
     6121 ccggaggggt ggtgaatgta gtcagcaagg ccgggaccaa cagcctgcac gggtcagcat
     6181 gggaattcaa ccggctctcg gcatacacgg cgaacaccta cgataacaat gctttaggcc
     6241 aaccaaaggg cgcctacact cgcaatcagt ttggatatga cattggcggc ccggttctga
     6301 aaaacaagat gttcttcttc cagagcaccg agtggacccg cgtgcgtagc cagccgacgg
     6361 aaagtcagct ggttcccact ccgcagtttc tggcctacac ggccccgaac gttcaggctt
     6421 attacagcgc ttatggagct acgccctatc cgatcacaac aacctattcg caatcggatt
     6481 taaacatatc gctgccgggc gtcccttcgt ccactcccat cctcgggcag gtcaactata
     6541 aggcaaatgc agatgcgggt ggagacgtcc cgcagaatac ttattccctt gtaggccggc
     6601 tggacttcaa tccgacagac cggacacaga tgtatttccg ctttgcccag caaagtattg
     6661 atcaatttgc aggcgcaact ttctacagcg cttatccgca atatgacatt ggacaaggca
     6721 catacaacaa cagcggtatc ttctccttta cgcatgcttt cagcccgagt gtgctgagca
     6781 acacaaaggc cagtctgtcc cggctgaaca cttccaatac ctataacaag gcaacgcaaa
     6841 atgtccccat gctgatgttc accagcaata ccgcgaatgg gttttccatt gccttcccgg
     6901 gacttcaaaa cctgggacag ccaggcgttg gtggctatcc tttcggcgga ccacagaaca
     6961 cactccaggc ccaggaagac ctctcatgga tgaagggcgg tcattctctc cgttttggag
     7021 gccagtacac ctatatccaa ctgaacaaag catatggcgc gtatgcacag gccgttgaag
     7081 tgcttggcaa tggcctgcag agtggactca acgccatgat ggcgggcacg ttgcaatatt
     7141 atgaagccgc cgtgaatccc cagggcaagt acccctgcat cacgaaccct gatggcagcc
     7201 agaccgtgac tccgagctgc gaggtcaaca cgcctgtcac atctcccagc ttttcccgca
     7261 gttatcgcta tcatgactgg gccctttacg cgcaggacag ttataaggct acgccccggc
     7321 tgaccctgaa ctatggcgtc cggtatgaac attacggcgt ccagcacaac gataaccaga
     7381 acctcgactc caatttctat ccggcagcgg gtggcaacta ttaccagtcc atccgaaatg
     7441 gacaggtgta tcttgctccg caaagtccaa tcggcagctt atgggcccca agctggggaa
     7501 cagtggcccc acgcgttggt tttgcctatg acgtcttcgg caatggttcg accagcctcc
     7561 gcggcggatt cggcatcagc tatgagcgca acttcggcaa tgtgaccttc aacgtcatcc
     7621 agaacgtgcc caattacgca acactgcaaa tcttcaacac gcctgtgacc accagcaatc
     7681 tcggcccgtt ggcgtctccc ggagtgcagc cattaccgag cacggaactg cgcatgcctg
     7741 atccgcatat caagacagcg cagacccagt tctggagcct tgcgctggaa cggcaggtac
     7801 tacgcaactc tgttcttgca ctggagtaca gcggcgcaca cggcgtccat ctgtatgaca
     7861 ttaccgtttc caacccgatt ggcggaggac agttctatct tggtgacccg ctctcttccg
     7921 ctgccagttg cccatacacc aatccggcca caggcgcagg ggcctgctat acccgtccca
     7981 attaccaata tgccggcatt aatacccgtg ggagccgcgg atccagtgca tatgaggccc
     8041 tgaacgtccg cttccagaca caagacctgc acaataccgg cttgagcatg acggccaact
     8101 acacctatgc ccattcgctt gatgacctga gttcaacctt cgccgacagt gcgcagggcg
     8161 gatccggcta tattggcaat cttggctatc tcgaccccac gaacccgggg cttgactggg
     8221 gcagctcgga cttcgacatt cggaaccgct ttgtcgtggc ccctatctgg cagaccccct
     8281 ggtttaagaa tgggaagggc tttgcacggc aggcgttggg aggatggacg cttgtcggca
     8341 tctttaccac tcgcggcggc acaccattct ctgccttcga ttattcctac aaccagaatg
     8401 gttatttcgg cgtctcgcgc cttgtgcccg cctcaccggt cacccaatac aaaaccggca
     8461 agggagtggc cgtcgggcca aaccagttcc aggttttgag cctgcctgca aacaacccgt
     8521 tcttcaaccc cgctctcggg ctctcggact tcgggccatt tccgagcaac atgacccgcc
     8581 ggaatgcctt ccgcggtccc ggcgcttgga acaccgatct ggcagccagc aagtcgttcg
     8641 cgctgacaga aagaatcggg ctgttgctcc gcgccgaagg ctttgacatt ttcaatcacc
     8701 acaacctgta cgtcaacgaa tctgcgctgg cggactttgc acctggcagt ccggcattgc
     8761 ccgtcattgc tctcaagggt ggattgaaca ccatcgccct gggtggcaat cacgacgagc
     8821 gccgcttcgg ccagttctct ttgcgagtta ctttctgaat ggatctattg gttgtgtcat
     8881 gaaaaaaggg tctggcataa taccagaccc tttttgattg gcactcaaag ctgtctcgac
     8941 ggaagtcctg ctgcgatgag tcaaaaattc tgaacgatgg aagaaacttc agaccctgga
     9001 cacttacagt ttctcgtaaa aatcacaagc cgagcacgaa atataaaccc cgcccggaat
     9061 gccccgctcg cggtctttga cgcgctttac gatgcgggta ggtttgctgc acttcggaca
     9121 gtccgtgctc ttggacgtgt ccattacctt cttgcggtct gctgtcttgg cgattttagc
     9181 catggaaact ctgtctcctc aaaaaatctt agatgtcgct atgttcaggg gcctggaaag
     9241 ctcgccaaca aagctcgggg cgtccggcct tccggagaga agaacatcgg aatctgcctc
     9301 tttattttac accaaaaact ggcggcaatt ggcagttgtt atccgcgcat ttcgggcagc
     9361 aaatttttcc tccccgtgtt acaaacgaaa gagcttcccc agtgaggtag tacgcagtaa
     9421 ctatgcccct tcaggaaaaa gtcttgatcg tcgaagacga ggagaacgcc cttaccggat
     9481 tggccgagct cgtctccggc tggggttacc gcaccgaaac cgcccgtgac ggactggaag
     9541 gcctggaacg cgctcaggcc tggcagccgg gaatcgtcat cactgacctg aaaatgccgc
     9601 gcatggacgg cctcgagctg ctggaacggc tcagcgagct taagcaggac aatcttgccg
     9661 tcatcgtgct taccgcgcag ggcagcattg aacaggccgt tgaggccatg aagatgggtg
     9721 cttatgactt catccagaaa ccgattgatg ccaaccgcct gcgcacgatc ctgggcaacg
     9781 ccagccggca gcgcgagacc cagcttgagc ttgaagttac gcgccgcaaa ctccgcgaga
     9841 ctggtgtgct cggccacctg gtcggctctt ctaaaaaaat gcaggagctt tttgctctca
     9901 tcgagcgtat cgcctcaaat aacgtctctg ttctgattac tggagaaagc gggacgggca
     9961 aagagctggt tgcgcgcacg ctgcatgacc tcagcccgcg caaggccaag ccttttgtcg
    10021 ccgtaaactg tgccgcaatc ccggaaactt taatcgaaag cgaaatcttc ggtcacgaaa
    10081 aaggcgcgtt taccggagcg ctggagcgcc gcgccggctg cttcgagctg gccgaggaag
    10141 gcaccctgct gcttgatgaa atcggcgaga tgcccatcgg cacgcaggcc aagctgctgc
    10201 gcgtgcttga agaccgcaag ctccgccgcc tgggcagcaa ggtcgaatcg cctgtggatg
    10261 tccgcgtgct ggccgcaacc aataaagacc cggaaaccgc cgtcgccgat ggccacctgc
    10321 gtggtgacct ttactaccgc ctgaatgttt tcaacattca tatgcctccg ctgcgcgagc
    10381 acaaagagga cattcctgaa attgtggact ccatgctgaa ggacatgaac gaaaagcatg
    10441 gacggcaggt cgccggcatt accgacgaca tgatggcgcg ccttatggcc tacgattggc
    10501 ctggcaatgt gcgcgaacta cgcaatacca tagaacgagc ggtaatttta tgcggcaacg
    10561 gctatcttga gccgaaacat cttcctcctg gtttcggcag caagcccgca aaacatgttc
    10621 ccgagagcgt tggaaatatt gtgcaggttg aggtagggac cacggtggac gaagcagagc
    10681 gccgcctgat tatgaagacg ctcgagtcca ccaacaataa taagaccagg gccgcggaga
    10741 tccttggcat cagcctcaaa acactgcaca acaagctgaa agagtatgga agcgccagcg
    10801 gcagccagga ggcggaagaa taacctgacg cggcgctcaa aaccctcttt cgattttcca
    10861 ggaatattga atgcggctga agacaaaact cgtttttgca atcaccggac ttgtatttgt
    10921 cgttgtcctg gtattttcct ggctttattt cagccagttg cttcagcagc acattgatca
    10981 gtcttacaac gccaccgata ttgtggccca ccagcttttg tttgccacgc gccaggcaat
    11041 tgaaaatggg acccgcaacc agcaaattga tcccaacgat ccggtggccc tgcgcgcagc
    11101 agtcgccagt gctctgcgca acgacgcagc gctgaacgcc ctcctcaatt ccgtcatcag
    11161 ctattcaccc accgttttcg atatctccat cgccgaccag aacggccggg tttatctgag
    11221 caccgatccc attctgcagg accagttcgt cccgcagcgt gtggcctatg cctcgctccg
    11281 gaaagcctct ctcgtgcaga ttctggaagt ggtcttcggg ccgccccggg tctacgatgt
    11341 ttcactctcg ctggagcgca acaaccagcc ttttgttacg gtgcagatcg gcattcgcac
    11401 gactttcctg agccatgcct ttcgcccgtg gttttttgag gcggccagcc tgatcggctt
    11461 tgctgttgtg atcacgctgc tggtatcggc catggtggcc aatcttgcct ttaaaccgat
    11521 cgaattgatc agcaaacggc tcgatctgct gacagcggag gtccagtcac aggaaaatga
    11581 tccagtcgtc gaactggaaa atccagacgg agacgatacc gtcttacagg tttctcacaa
    11641 aattgagcgc ctcggacgac gcatgaaaaa cgtcgaagag gtcttctcgt ctcttaaaca
    11701 aaatcttgat caaatcctgt ctaacttgca ggatggcatg attttattca cgagggatgc
    11761 gcgtgccgtt cttgtgtcca gctccgtcga gcggtttctc gccaggaacc gcgatgagat
    11821 cctcggagca agcgcccatg acatcttcga ccgctcaacc atcctgggcc gggtggtgcg
    11881 cgaagccttc gaccgtggaa tcgggctcgt gcaggaagaa attacgacgg aaaccggacg
    11941 gcgcgtggaa gtctccctcg atttcattca cgacgacagg gttccccagc agcgcgacgg
    12001 cctcggcgca ctccttacgc tgcacgatct tgaatccgta caggaaattg agtctgagct
    12061 ggagatttcc cgccgcatgg ccgccatcgg ccaattgact tccggcgtgg gacacgaggt
    12121 caagaacccg atcaacgcaa tcgtcgtgca tcttgagctg ttgcggacca agatgacggc
    12181 ccccgatccc tccgccacgc gccatctcga tgtgatccag aacgaaatcc agcgccttga
    12241 ccgcgtcgta caaacgctgg tggatttctc acgcccagtc gaactcaagc tccacgagca
    12301 ggaccttcgt ccggtagtga atgcggtgct ggccctggct tcggtcgagc tcgaaacgcg
    12361 caatgtgcgc gtgctcaact ccatgccgaa ccatccggtc atcgccaaaa tagacgcgga
    12421 cctgatgaag caggccatcc tcaatattgt gctgaatggc gcgcaggcca tggtgcgcgg
    12481 tggcatactg cgcgtcaacc tggcagaaga tggccgctgg agctatctaa gcatcagcga
    12541 tgagggcgaa ggcatctctc cggatgtccg cgaaaaaatc ttcaatctct atttcacaac
    12601 caaaaaagaa ggcagcggca ttggacttgc catgacctac cgcatcgtcc agatgcataa
    12661 tggccagatt gacgtagaat cagaagtagg caaaggaaca accttcacgc tgcgggtgcc
    12721 tgccttcagt acttcagaga atagattgcg cgggcatctg gaagcagaag cggccaaaag
    12781 cgcatcgttt gttaaggagc cttggggatg aacaccgctt tccgcactgc ggtgatcgta
    12841 tcagcatgtt tgctccccct ggcggggtgc agtcacagga acaaagtact gcctccgcag
    12901 caggcccagg cacccgtgcc gcctccatcc atgatggtgc attatccggc cctcccgccg
    12961 ctgcctccgc caagcgcccc ccgggttgag ttggccagcg tacaggaaac gcctccggcg
    13021 ccgcgcccgc atcgtgcttc acaccggaag ccatctccgg caaagactgc gccgggcact
    13081 gaaactgccg cggaaaaaca acctcagcag cagactactg aagtcgcatc tgctgccccg
    13141 gcgccggacg cttctcccat tggacaactc acagcagctg gagacgcaac gaacattcag
    13201 cagcgccgcg acatcgaaca gctcatcaca aatactgaaa acggactgga caaaatcagc
    13261 cggaacctga acgccgatga gcagaagacc gccgtccaga tccgcacatt tctgacaaag
    13321 gccaggcagg cactgaccga taacgatctc gatggcgcga acactctggc gaccaaagcc
    13381 aaagtgcttc tcaatgagct tacaaagaaa taagcgtaga cctctgcagt cccctgctta
    13441 cttccaccac gtgtacccaa tctgaaactg catactggca ttcactccag ggttgcggtc
    13501 gcctagcgaa gcactggaaa tgtggaccgc attcacgcca aatgtgaggg actgccggtc
    13561 ttttacgaag taatgaaaac caattcccgc ctgtggagta aagttccaca cgctcgttcc
    13621 tcccgcatcg ccatgctgca cgataatgtc cggcgggtat ttgtggttgg tccagataag
    13681 gccgccggct ccctggaacc acggcgcaaa tcgcctgccc ggcttgaagt tccaacgcag
    13741 aataatcggc gtaatgctta cgcccgtgaa cgttccaccg ttatacggca gcagcgcctc
    13801 acctgtttgc ccctcaaagc tgtattttgt ccattgcaga tgaggagcgg gagtgaacga
    13861 ctgccagtag ggcataaact cgcccgcgta ttcaaactgt ccgcgcagaa tgcctggcag
    13921 atgttcgtct gtcatcactt ttcccaggcg catacccgcg ctggcaaagg tgaaatcgga
    13981 gcggtcgcct acacccactc cgccttgaaa aaatggccca aactcccagg gacgggtggc
    14041 cagcgccgct gaagccggac tgctgttggc cgcagcaggc ttgccctgcc cgaccatctg
    14101 aagtgaaaac aaagaagaag caacaacagc aaagcaaacc gcgagttttt tgcgcatgat
    14161 gtgtgtattc tttatcggat ttcaaggatg aagtgtaaaa gaatggccgc ccagggcggc
    14221 cctcttgttt cattcagaaa agatcagcct gcgacctctt caagttgttt ggcgtccata
    14281 tcaaaattcg agtagacgtt ctgcacatca tcatggtctt ccagcgcttc gagcaaccgg
    14341 accatctgcg acgcggcagc accttccagt ttcgtatagg tcgaagcgat catcgtcacc
    14401 tcggacatca ctgtctcaat gcccgcatcg cgcactgcct gtgcgacggc ttcaaaattc
    14461 tgcggctcgg tgatgatctc ccatgtgtcg ccttcgtcgt tcaggtcctc gccgcctgcc
    14521 tcaagcacaa tgctcatcag cttttcttca tccgctgcgg tcttcggaac ggcgatgatc
    14581 ccctttttgg taaacataaa gcgcaccgac ccggtttcac ccaggttccc gccatgcttt
    14641 gagaaaagat gtcggatttc gctcactgtg cggttacggt tgtccgttgt agtctccaca
    14701 ataatggcaa cgccaccggg accatatcct tcaaaagcga tctcttcata ggttgcccca
    14761 ggaagctcac ccgttccgcg ctggatggcg cgcttgatat tgtccgcggg catgttttct
    14821 gcttttgcag cagcaatggc ggtgcgcaga cgcggattgc cgtcagggtc tccaccggcc
    14881 cttgcagcga tggtgatttc ctttatcagg cgagtaaaaa tcttgccgcg ctttgcgtcg
    14941 agagcacctt ttttgtgctt gatactgtgc cattttgaat ggccggacat gggaaacctc
    15001 ggaagctgtt tttaggattg aacctgcggc gaaatgctga aactggtatg gcttagagca
    15061 atctgccgca acagttaagt atagcaggac ggcccgttct cctactctgt gtataaattt
    15121 catttatagc cttactgaat ccttgcaaac tgcttatgcc cctttggcga cgctttggta
    15181 aggctggtct gtttgaaaaa acttcctcgc tcaggagatg tcaccgatga agctgttccg
    15241 ccgtgctctg ttctttcttt ccgcgccgtg cctgctgccg ctccatcctg catgggggca
    15301 aaccacttcc cgcgcagagg agatccgtga gcttcgccag cagcttgcca acctgaatgc
    15361 acggctcaca aagctggaaa acgaagaatc cgcaggcaca actcccgccg gagatcccac
    15421 agcaaatgcc gctgccttaa gcgccgccag ctcggcactg acagcacaac cggagccgga
    15481 atccacgcag tcaaagcagc tcacgagcgc tgaccggagc acgctcgact ccctgaacgg
    15541 gaccactttc accatgggcc tcgatggcta ttacgaatac aacttcaatg acccaatcgg
    15601 ccgcgcaaac ctgcttcgtg tgtatgacgt cagcagcaac gccttcagtc tgagccaggc
    15661 gaatttcatc attgagcatg ctccccatcc ggagcacggc cagcgcttcg gagctcggct
    15721 cgatgtgcaa tatgggcagg caacacagag tctccagggg aatccctcca acgagccgcg
    15781 ttccagtatc taccgaaata tctttcaggc ttatggaacg tatgtggctc ctgtcggcag
    15841 tggcctcacg atggatttcg gcaaatgggc cagcagtgtg ggcattgagg gcacctatac
    15901 caaagaccag atgaactact cccgctccat ctggttcgga tttctgccct cctatcacat
    15961 gggtgtccgc ctgaattatg gattcactcc ctggctctca cttcgctacg ccactgtgaa
    16021 tggaatccag caaacagaag cattcaacgg attcaaagac cagatgtttg atctggacat
    16081 caagccccgc aaatcggtgg actggaacat taactattac ctcggccagg aacatcctga
    16141 tttccaatac gtgacaaacg gccccggcac tcttcccacg cagcagggca tgccatttga
    16201 accgataccc catcccgcca aaggcaaaat ccatatcttt gacagctacg ccacatggag
    16261 cgccacgcaa aagttgaccc ttgctctcga aggcgattac gctatcagcc gcgaccagac
    16321 ctggtccgct ccgcagcacg tagatggggg cgcggcctat gcgcgctacc agttcaaccc
    16381 gcaatttgcc cttgcagcac gcgctgagta ttttgctgac ccgaatgccc tctacaccgg
    16441 aaaatcgcag gccatcaagg agttcaccct caccttcgac cacgctctca ccaatggctt
    16501 cctgatgcgc gaagagttcc ggcacgactt ctcgaaccag ccgtatttcc tgacaagcac
    16561 actgggcagg ctgaatcacg accagaccac tgccaccctg ggtcttctct ggtggttcgg
    16621 aggaaaacag ggagcgtggt gaaccgcgtt ctctccaaga tgaatggacc actctgtttc
    16681 gtatagcggt acggcaataa agaccgggta ttgacagtcc tttgttgccg tctctatacc
    16741 gtcatctcag aacggaaaga attccccatt tgaaaagggg tggtctatgc tgcgcaataa
    16801 aacgtttatt ctggccagtg cgcttttcgt agctcttctt attgcccttt gcccagtcct
    16861 gctgctctcg caggcggtct ctgtggcctc tgtcgccggt cgcgtgaccg atccgcaggg
    16921 cgcggcggtg agcggtgctg aagtaaagat gacctcgacg gaaacgggcg cggtgcgcag
    16981 cgtcatcacc aacagcgaag ggctttacag catccccaat cttccgattg gcggatacac
    17041 actcgaggcc tcggcccccg gcttccagac ctatgtgcaa aaggaaatct ccctgcgcgt
    17101 caacgacaac attcagatca atgtttcgct gagtgtcggc aatgtttctc agcaggtgac
    17161 ggtcagcgcc gccgcacaga tggtgcagac gcaggacaac agcatctcgc aggtcattga
    17221 ccagcaccgc atcgccgagc ttccactcaa tggacgtcag gcaacacagc ttatcctcat
    17281 ctccggcgct gccgtcacca cacccggcgg cgacatgacc ggcagcaaaa acttctacag
    17341 ctcgacaacg atttcggtcg ccggcggaca ggcaaaccag ctcaattatc tgctcgacgg
    17401 cggcgacaac aacgatacct tctccaatgt aaatctaccc ttccccttcc cggacgcgct
    17461 gcgcgaattc agtgtggaaa ccaatgctct gcctgcgcgc aacggattgc atccaggcgg
    17521 cgtggtgaac atcgtgaccc agtcgggcag caatcagttt catggagttg tctttgagtt
    17581 tctgcgtaac aacgtgctga acgccacgaa cttcttcagc accaaacgcg acacgctcaa
    17641 gcgcaaccag ttcggcggca ccttcggggg acctgtgatt cacgacaagt tcttcttctt
    17701 tggtggatac cagggaaccc gcaaccggca gacaccgaac tcggcaacag cctatgttcc
    17761 tagccaggca gcgctgaatg gcgactggtc ttcgatggag tcggcgggtt gccagtccag
    17821 cggaaaagcg cgcgtcatca aaaatcccta caccggtcag acatttgcca ataaccaggt
    17881 gcctacgtcg ctttacaacc cggcggcgct tgcactgttg cagtacctcc cggtcagcag
    17941 tgacccctgc ggcaaggtgg tctacggcat tcccactacc ggagatgaag accagggaat
    18001 cctccgcctc gactatacct ttagtcccag gcagcagctt tatggccgct actttatcgt
    18061 ggactatcgc aatcccgccg tgtttgactc taagaacatt ctggtcacga cgaaccccgg
    18121 caatctcgag cgtgcgcagg cattcacgct cggcgataca tggacgatct ctcccaacct
    18181 cgtaaattcg gcccatgcaa caattacgcg gcggcgcgat gaccgtgggc ctgccccaaa
    18241 catgatcaat ggcggcaaac tgggcatcaa cacgtatttc tatgttccga atgatctgcg
    18301 gctttccgtc agcaacggat tcaacacgag ctgcggaaca tgcgccccgg gcttcttcaa
    18361 cgtcaccacc ggacaagccg cagacgatgt ggacttcctc aaagggcggc accagttcgc
    18421 ttttggtgct gacttcatcc gtacagacaa caaccttgaa accggatacc tggagaacgg
    18481 ctactaccag ttcaatggac aatacagtaa tgacccgctg ctcgatttca tgctcggcgc
    18541 gatgaatggc tatggccaga gccgtccgca gcaggtggct atgcgcgaaa gcatcatcgg
    18601 cgtctatggg caggacacca ttcacgtcaa cccgcatctg gtcgtcaaca tcggcctgcg
    18661 ctgggagccg cagctcttcc cgcatgatta tttccaccgc ggcagtacat tttcgcaggc
    18721 cgcatttaat gccgggcaga agagcaccat cttcgccaat gctccggcag gctcttttta
    18781 ctatggagac aaaggcgtcc ccgcggcctt tacaaaaaac acgctcacca atttctctcc
    18841 gcgtttcggt ctggtctttg atccacgcgg cgatggacgc caaactttcc gcgccgggtt
    18901 tggcctgctt tacgactccg gaatgctcta cgccacacag cgcctgacgt cgaatccgcc
    18961 ttttgtgaat gaaattgatc tgacaggcgc caatggcccg ggcggctcta ttccgtttaa
    19021 tcagccgtgg tctgcatatg gcaacattcc cttcccggcg ccctatccgc caccatccga
    19081 tgtcagcttc ccgcagttcc ctctcttcgt cgtgctgcct gatcgcgtaa aacccaccta
    19141 catcgagcag tggaacgcaa gctatcagtt aggattcgcc aacaactggt tgttctcgat
    19201 gtcttatctt ggcaaccata caatgcacgt ctggggcgga ctgcccatca atccagcggt
    19261 ctatattcca gggaactcca cgaccaaaaa cacaaaccag cggcgtgttc tttatctcgc
    19321 caatcccagc cagggccagt attacggaag catctttcta ttgcgcgacg aaggtgttgc
    19381 gaattacaac ggaatgctcg tctcggttga acaccgcatg tcgtcgaact ttaccgtgct
    19441 cgccaactac acctggtcta aatgtctcag cgatacagac tttgacggtg acatccgcca
    19501 atccaattac cagaatccag cgaatccccg tgcggattat tctgtctgca atttcgatgt
    19561 cccaaatctg ttcaacctca cggctgtcgc catcagtcat ttctccggcg gctggaaaca
    19621 aaagattctc ggtaactggc agattgcgcc gttgattcgc atctccagcg gaatgcccgc
    19681 caacattacc tcaggccagg actactctct cacaggcaca ggcatggacc gtcccaatcg
    19741 cataggcaat ccttatcttt ctcaccgcac ctgcacgcag gccgcctgcc agtggctgaa
    19801 tcctgcctca tttgcaaaga acgcggcagg tacattcggc aatatgcctc gcaacggtgt
    19861 gcgcaccccc acctatgctg acgtggacgc agccctcagc cgcatctttc cgattacaga
    19921 gagatggcag attgaaggcc gcttcgagtc gttcaacttg ttgaaccatc cgaattttgg
    19981 aaacacagca ggcaatctgt ccacaaacct taattcctcc tccactttcg gacggatcac
    20041 tgcggcaggc gatccacgca ttatgcaggc cgcgctcaaa atccggttct gacacataaa
    20101 accgtgtgct ggggctacac catccctctt ttcacttcac tgaaattttt ccccgccttc
    20161 tgtccctatc tgaatggaca aagccgtccg caaggaggaa actgtctccg ctgcctgctc
    20221 tcccctgacg gcccaacttt acaggaaaag aaggaacccc atgaaaattc tgaaatatgc
    20281 cgtgctcgcc ctgggcatgc tctttcccct ctccgcgttt gccgacttcc agtatcagga
    20341 aaccacccag atcaccggcg gctctcttat gaacatgctg aaaatggcca gcgtcttcag
    20401 ctcgcgcgca agagaccaga tgaacgctcc ggttgtttcc gctgtgtaca tccagggaaa
    20461 ccgcatggcc cgcatcaacc agtacacgat ggaaatcatt gatctcgata aagagaccat
    20521 caccaacgta gacctgcaaa aacacaccta tacgcagatg acttttcaac aaatgcgcga
    20581 agccatggag cgcgaaatgc aaaaggcccg cgaacagcag gccaaacaaa gcggagacca
    20641 atctcaggcg gccaacaacg tagatatcca gttcaaaatc aatgtgcgca acaccggagc
    20701 aacaaaacag ctcggcggcc ttgagacaag cgaatccatt ctgaccatgc agatggtcgg
    20761 caccgacaag accaatggcc agcagggcgc cttcgccatg accaacgata tgtggcttac
    20821 ccccagtgtt cctggctatg aggaagtacg tgagttttat aagaaatacg cgttgaagat
    20881 gggccagaca ttcaacggga gcaacatctc gcagtccctt atggccatgc atcccggtat
    20941 gtcgcagggc atgtctgata tggcgaagga agtggagaag atgaaaggca ttcccatgct
    21001 gcaagtgctc cgcataggcg caacagccaa cggcgatcct ctgccggcag ccagtgaagc
    21061 tcctctgcct gcttcttccg gcccgcaaac gccttcagcc ggagaaatcg cgcagcgctc
    21121 tgccgcctcc gcaatcgcca gcagactcgg cggattcggg cttggtgggt tcggccacaa
    21181 aaagcaacag cagcaggacg gctccgcgaa caacgcccag gccaatgcag cacagcagca
    21241 gaccgcatcc gtcctgatgg aaatgcaaac tcagatggac tccttttctt ctgcccccat
    21301 tgatgcatcg aaattcgcag ttccggcagg cttcaaacaa atcgagccgc agatgcacta
    21361 aggccggccg ccacgcatcc tacttgccgg aagcgaaggc gga
//
LOCUS       DASRBV010000024         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_507882, whole genome shotgun sequence.
ACCESSION   DASRBV010000024 DASRBV010000000
VERSION     DASRBV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507882"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VFA02_01080"
     CDS             <1..1026
                     /locus_tag="VFA02_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosiduronase"
                     /protein_id="HZU08467.1"
                     /translation="LDAEQIAKEWTRLTFGNDPQVVKTISGMQLASWHIYESYTGPLG
                     AGTMTDITGPHYGPGIESAERNGWGQWFRADHQGIGMDRTVATGTGYIGQYHQPVAAM
                     YESLKTCPDNLLLFMHHVSYTYRLHSGQTVIQYIYDSHYWGAEAAASLVRQWQTLKGS
                     VDDERYDEVLKRLTYQAGHAIVWRDAICEWFLKMSGIPDEKGRAGHHPNRIEAETMQL
                     DGYTSVDVTPWETASGGKAIACNRASGCSATFQFEGRPGEYDLATQYFDLNTGTAHFR
                     LYVNGKLVDLWSADDTLPSEQMNGHTSTRHTIHNLALKPGDIIRIEGIPGGGDLAGVD
                     YIEELPL"
     gene            1237..3258
                     /locus_tag="VFA02_01085"
     CDS             1237..3258
                     /locus_tag="VFA02_01085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZU08468.1"
                     /translation="MNDRKKLSLFFSLASTLLTFPGLQAQTQIGPPKITMMSSDHGPA
                     QPSKKFTVTVHLKMHNEDAFDKALQALYTPGSPTWHKWMTAKDLAKYASTPEEIKAVK
                     DELTLHGLSVLSISPDNLSIRARGTVASMEDAFQTQIHEFEREGRVFHANVTSAKLTG
                     NAGNLIKSVSGLSSFPLRPMIQHPVDPITGKKLPTIPLADVPVGGGGLSDHFSNNCFP
                     GPASLNLTMLSGASLPVGQYYGNIYKSEALGKNHICAWTPGQVQAHYGLTSAYSKGID
                     GTGQTIVIVDGPSDPTVKDDLTQFAQLTGLPAITSSNFQIIYPDGQPSQWELSSVANW
                     DEEADLDIEWAHAIAPKAKIVLLITPTQDWSELEYAIQYAQEHKLGNVISNSYGYPEM
                     AWGAHTLEGFNQVLKLAAAQGIAVNFASGDYGDMGTGSPSGGGTEFPASSQYVTAIGG
                     TSIGIPDGSSHGAEIAWGHNAVVIANGYGVLDPPILHGFASGSGGGISGFIAKPSWQK
                     SLSGSYRQVPDISALADPYTGAIIVIGGQAGAIGGTSLACPIFSAIWALADQKAGRPL
                     GQAAPLIAKLPTGAVKDIIPFSSPTNPAGVVFDSNGSTYYSSDALLAPLYTTTAYYSA
                     LWFDQNIPGYVILSFGTDSSLTVTPGWDNITGWGVPNGWTFITAAAEKK"
     gene            complement(3329..3868)
                     /locus_tag="VFA02_01090"
     CDS             complement(3329..3868)
                     /locus_tag="VFA02_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08469.1"
                     /translation="MLPRIRLLLLPLLCFVLFAQQGDSLRPAVFPNLNAENLAKTKLN
                     LPSDFAGKANLLLIYFEPEQAKVVDTWMPFAQALQHMNFNFRYYKMPVSNPENLIFRW
                     WDTSSLRSVEADPETWPWIIPLYTNKQDFRRALEIPNEKDIVAVLVNKSGQVLWRSSG
                     KFTDDKKGPLSAAVAAATR"
     gene            4001..>4935
                     /locus_tag="VFA02_01095"
     CDS             4001..>4935
                     /locus_tag="VFA02_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HZU08470.1"
                     /translation="MTSLSSQRPLATTDAQLAPIAEKVFSGERLSFDDGLTLYRSRDI
                     FAVGWLANYVREKLHGDIAYFNVNRHINPTNVCVAACRLCAFGRKKDAPGAYTMALEE
                     AWAAAASGYTEAVTEFHIVGGLHPDLPLEYFVNLVRGLKERFPQVHIKAFTMVEVSFL
                     ARRAKLSIRETLEKLRDAGVDSLPGGGAEIFADRVRHIICDHKIDGDEWLETARIAHE
                     LGLKSNATMLYGHVENDEDRVDHLLKLRALQDDTHGFQTFIPLAFHPDHTPLEHLPRT
                     TGMMDLRQIAVSRLLLDNFPHIKAYWQMMTPQIAQ"
BASE COUNT         1161 a         1464 c         1286 g         1024 t
ORIGIN      
        1 ctggacgcgg aacaaattgc caaagagtgg acacggctta cctttggcaa tgacccgcag
       61 gtggtcaaga cgatttccgg gatgcaattg gcctcgtggc acatctacga gagctacacc
      121 gggccactcg gcgcgggtac catgaccgac atcaccggcc cgcattatgg cccgggcatt
      181 gaatcagccg agcgcaacgg atgggggcag tggttccgcg ccgatcatca aggcatcggc
      241 atggaccgca ctgtggcgac aggcacgggc tacatcggcc agtatcatca gccggtggct
      301 gcgatgtacg aatcgctgaa gacctgcccg gacaacctgc tgctcttcat gcatcacgtt
      361 tcatatacat acaggctgca ttcgggccag acggtcattc agtacatcta tgactcgcat
      421 tattggggtg cagaggccgc ggccagtctc gttaggcaat ggcagacgct caaaggcagt
      481 gtagatgatg agcgttatga cgaagttctg aagcggctga cctatcaggc aggacacgca
      541 attgtctggc gagacgccat ttgcgaatgg ttccttaaaa tgtccggtat ccccgacgaa
      601 aaaggccgcg caggacatca ccccaaccgc atcgaagccg aaaccatgca gttggatgga
      661 tacacgtctg tagacgtcac accgtgggaa acggcttcag gcggaaaggc cattgcctgt
      721 aaccgggcaa gcggctgctc ggccacgttc cagttcgagg gcaggcccgg cgagtacgat
      781 ctcgccactc agtactttga tctgaacaca ggcaccgcgc attttcgact gtatgtgaat
      841 ggcaagctcg tagatttgtg gagcgcagac gacaccctgc cctcagaaca gatgaacggc
      901 cacacctcca cgcgccacac catacataat cttgcgctca aacccggcga catcatccgc
      961 atcgaaggca tccccggcgg cggcgatctt gccggcgtag attacattga agagcttcct
     1021 ttatgagttc cgggtggcag cgctaagctc agaaagaaac cacacggtag caaatatctc
     1081 ttctttatcc ctcatttttc tcattgtctt tccaggtgct tgctgttatc tcttacgttc
     1141 aatggatttc cgttcaaaca attgaatccc gaacaggcgc tgacctgtgc cttccatcca
     1201 tgattttcca accttcagga gatgaaagtg ctgttcatga atgaccgaaa gaagctctct
     1261 ctgttttttt ccctggccag tacgctgctc acctttcctg ggctccaggc gcagacacaa
     1321 atcggaccgc ccaaaatcac gatgatgtcc tctgaccacg ggcctgctca accctcaaaa
     1381 aaattcaccg tcaccgtgca tttaaagatg cacaatgaag acgccttcga caaagcgctg
     1441 caagcgctct atacgcccgg ttcacccacc tggcataaat ggatgaccgc gaaggacctg
     1501 gcaaagtatg cgtcaactcc agaggaaatc aaagctgtaa aggatgagtt gaccttgcat
     1561 gggctttcag tcctctccat cagcccggac aatctctcca tccgcgcacg cggcaccgtt
     1621 gccagcatgg aagacgcatt tcagacacaa attcatgagt tcgagcgcga aggaagggtc
     1681 ttccacgcca acgtcacgtc cgcaaaattg actgggaacg ccggcaatct tatcaagagc
     1741 gtatcgggtc tgagcagctt tccgttaagg cctatgattc agcatccggt ggaccccatc
     1801 actggcaaaa agctcccgac tattccgctc gcagatgtgc ccgtaggggg tggcggtctg
     1861 agcgatcact tctcgaacaa ctgctttccc ggccctgctt ctctgaatct cactatgctc
     1921 tccggggcct cgctgcccgt gggccaatac tacggaaata tctacaaaag cgaagcgctt
     1981 ggcaaaaacc atatctgcgc atggaccccg ggccaagtgc aggcgcacta cggcctgacc
     2041 agtgcttaca gtaaaggcat tgatggcaca gggcagacca tcgttattgt ggatggcccc
     2101 agcgacccca ccgtaaaaga cgacctcacg caatttgcgc aactgaccgg acttcccgcc
     2161 atcacatcat ccaatttcca gatcatctat cccgatggac agccgagcca gtgggaactg
     2221 agcagcgtgg ccaactggga cgaggaagcc gaccttgata tcgagtgggc gcatgccatc
     2281 gctcccaaag cgaagattgt tttgctcatt accccaaccc aggactggtc agagcttgag
     2341 tacgccattc aatatgcgca ggaacacaag ctcggcaacg tcatctccaa cagctatgga
     2401 taccctgaga tggcctgggg ggcacatact ctcgagggct tcaatcaggt gctcaaatta
     2461 gcagcggccc agggcattgc ggtaaatttc gccagcggcg actacggcga tatggggacc
     2521 gggtctccct ccgggggcgg gacggaattt cctgccagct cccaatatgt caccgcaatt
     2581 ggtggaacat ccatcggcat ccccgatgga agcagccatg gggcggagat cgcatgggga
     2641 cacaatgctg ttgtgatcgc caatggttat ggtgttctgg acccgcccat cctccatggc
     2701 tttgcatccg gttccggtgg tggcataagt ggattcatcg ccaagccttc atggcagaaa
     2761 tccctctccg gatcgtaccg gcaagtaccg gatatttccg ccctcgccga cccatacacc
     2821 ggggccatca tcgtgatcgg cggacaagca ggcgcgatag gcgggaccag tctggcttgt
     2881 cccatctttt ctgcaatctg ggccctggca gatcaaaagg caggcagacc cctcggccag
     2941 gccgccccgc tgatcgcaaa acttcctacc ggggcggtga aagatatcat cccattcagc
     3001 agtccaacga atcccgcagg cgttgtcttt gattcgaacg gctccactta ctattcttct
     3061 gatgctttgt tggcaccgct ctacaccacg accgcgtatt acagcgcgct ctggtttgac
     3121 cagaacattc caggctacgt catcctcagt ttcggcacag attcatcgct cacagtgacg
     3181 ccaggctggg acaacataac cggatggggc gttcccaacg gatggacatt catcacagcc
     3241 gccgccgaaa agaagtaact ctacaaagca ggtccccctt tttgccatcc tggcactgag
     3301 cgtagcgaag ggcaaggacc tgcttttttc accgcgtggc agcagcaacg gccgccgaga
     3361 gcggcccctt cttatcgtcc gtaaacttgc cggaagagcg ccagagcacc tgtccgctct
     3421 tgttcaccag cactgcgaca atgtccttct cattgggaat ctccagcgcg cgccggaaat
     3481 cctgtttatt ggtataaagc gggatgatcc acggccacgt ctccgggtcg gcctccacac
     3541 tgcgcagcga tgatgtgtcc caccaccgga aaatcaggtt ctccgggttg gagaccggca
     3601 tcttgtaata gcggaaattg aagttcatat gctggagcgc ctgggcaaat ggcatccagg
     3661 tgtccacgac cttcgcctgc tccggctcaa aataaatcag cagcaggttt gcttttccgg
     3721 caaagtccga cggcagattc agtttggtct tcgcaaggtt ctcagcattc aggttaggga
     3781 agaccgcagg gcgcagagag tctccctgct gagcaaacag cacaaagcag agcagcggca
     3841 atagcagcaa tcgtatgcga ggaagcaatc aacaagacct taggtaacag aattctggaa
     3901 atttcttctt tgtggcatgt taccagtcac caggaccccg ggtgaaccgg gaagcgacaa
     3961 aacgatgcaa acaagtatca taaaaggaag gacataggcg atgacttctc tatccagcca
     4021 acggcctctt gctactactg acgcacagct tgctcccatt gcagaaaagg tcttttctgg
     4081 tgagaggctg agtttcgatg acggcctcac actctaccgt tcgcgcgaca tctttgccgt
     4141 gggctggctg gccaattatg ttcgcgaaaa gctccacgga gacatcgcct acttcaacgt
     4201 caaccgccat atcaatccca caaacgtctg cgtggcggcg tgccgcctct gcgcctttgg
     4261 ccgcaagaaa gatgcaccag gagcctacac catggcgctt gaagaggcct gggccgcagc
     4321 cgcctccgga tataccgaag ctgtcaccga gtttcacatc gtaggcggac tccaccctga
     4381 tttaccgctc gaatactttg tcaatcttgt tcgcggcctc aaagagcgtt ttccgcaggt
     4441 acatatcaag gccttcacca tggtagaagt ctcgttcctt gcccgcaggg caaagctttc
     4501 gattcgggag acactggaaa agctgcgcga cgcaggagtg gattcactgc ccggcggtgg
     4561 cgctgaaatt tttgccgacc gggtccgcca catcatctgt gaccataaga ttgacggcga
     4621 cgagtggctg gaaacggcgc gcattgcgca tgaactgggc ctcaaatcga atgccaccat
     4681 gctctatggc cacgtagaaa acgatgaaga ccgcgtagac catctgctga agctgcgggc
     4741 ccttcaggac gacacacacg gtttccagac tttcatcccg ctggccttcc acccggacca
     4801 cacaccgctc gagcaccttc cacgcaccac aggaatgatg gacctgcgcc agattgccgt
     4861 cagccgtctg ctgctcgaca acttcccgca catcaaggcc tactggcaga tgatgacgcc
     4921 gcagatcgcg cagat
//
LOCUS       DASRBV010000025        22681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_558313, whole genome shotgun sequence.
ACCESSION   DASRBV010000025 DASRBV010000000
VERSION     DASRBV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 22681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22681
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558313"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1033)
                     /locus_tag="VFA02_01100"
     CDS             complement(17..1033)
                     /locus_tag="VFA02_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZU08471.1"
                     /translation="MRLRDLLQWIVLLLPVSLPAQQQCALCHPREAWAQPRTPMANAM
                     QLAPLDLLFRTHPKLIFQQNGYTYSLEAREGHVTYTVSHGAQSISLPVKWALGAGNQT
                     WVIERNGKFYESLVSYYLSTDGLNITTGDEKLTPRNLEEAFGRELSPAEAMTCFHCHA
                     TNAIHEDRLALDGVHPGITCERCHQGAEAHSHDMMQGNRSSIPPDLGSMSSEDISNFC
                     GQCHRTWEAVIRSRERGPVDVRFQPYRLANSRCFNGNDPRISCIACHDPHGPLSHDGS
                     YYDAKCLECHTPEARTAHIRSCPVSSSKCVSCHMPKVQLPNGLLSFTDHQIRIVRPGE
                     PYPN"
     gene            complement(1020..2132)
                     /locus_tag="VFA02_01105"
     CDS             complement(1020..2132)
                     /locus_tag="VFA02_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08472.1"
                     /translation="MLGKHFGLWMVMLLLCSAVTARAQNGLSFDELVAKAAQAREQHD
                     ATDAISLYQQAVELNPKWAEGWWYLGSLQYANNDYAHARNALTHFIANNPTAGPAFAL
                     RGLCEYETGDYAQSLADLQRGLSLGAANRPENTRILRYHEAILLTLSGRFEEAMDRFT
                     FFAQNGISGPDIFLAIGLAGLRMPLLPKDVPAAKQDLFLAAGRATWIFMTGNTAGAQQ
                     AFQDLFQGSHDAENVHYLYGYLLFPSDPDAAIKQFRQEMQHYPANASCAAILAWAYLI
                     QNEPRMALPYAQEAIEELPSQMLPHLILGKALVETGNISAGTVHLEEALKMAPDNLEV
                     HIALAKAYSKSGRSEDAFQQRLWCLHATAGEQHETP"
     gene            complement(2132..3745)
                     /locus_tag="VFA02_01110"
     CDS             complement(2132..3745)
                     /locus_tag="VFA02_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU08473.1"
                     /translation="MLLSQNRPQQGLIRFDLKRIPFRLENDETPGRNAPESMAGGVAV
                     FDYNHDGRPDIFFTNGANIATLKKDSPKYSNHLFRNDGNSVFTDVTKQAGLEGSGYDI
                     GVAVADYDNDGCTDIFIAGVFHHTLYHNNCDGTFTDVTLKAGLDKSIDPQYGPLWSVA
                     AAWVDVNNDGLLDLFVVNYMQWKYSSQPLCSFRGVADYCHPRYYKGQPSQLFLNNGNG
                     TFRDVSKEWGIRDHVGKGMGVGVADYDHDGRSDLFVTNDAEYNFLFHNLGSKFEEVAF
                     QTGVALAEDGAFISGMGLDFRDYNNDGWPDIVFVALNNQTFPVFQNTGKGDFQEVTNA
                     SGMRVLSASMAGYGAGLYDFDNDGWKDLFVTRGHVESQPLPGTEIDQPNTVFRNLGPS
                     GKWEAFTAEAGLTAAPPARHRGCAFGDFDGDGRVDVIATALGQQAEIWMNRSPHSGHW
                     LDIALRGVKSNRDGIGASIKIVSKSGIQFNHMTTSVGYASSSDGPVHFGLGADTRVGL
                     VEIHWPSGIVQTLHDIAADQVLTVVEMQK"
     gene            complement(3871..7611)
                     /locus_tag="VFA02_01115"
     CDS             complement(3871..7611)
                     /locus_tag="VFA02_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08474.1"
                     /translation="MAEFSRVLSACFRNTTGKFRKQFLWVLLAMATSGFLLPSVRAQQ
                     LTGTISGIVYDQSGAVVPNAAIELRNQSSGDLRVSKSNNAGVFTITAVPPGTYTITIS
                     ATGFTTWQEQGVVMNQGDNRSLPNIALKVGSSSTSVQVVSSEAAVIPMDTGEVSTTLS
                     TEMIQDMTLGGRDAGELLKLIPGAAFTNGLTQGSSFNPKITGTNNGPVGAYSLNGTQP
                     YGSMAFMLDGANLVDPGNAGTQIANINQDMTSEIKILTSSYGAEFAKGPVIFQAFSKN
                     GGQAFHGEGYFYARNSTFNSIESYAKSQIAGNIKNYIANGMSASQAKSTALKQALPDE
                     HYYYLGGNIGGPVLFPFTGFNKNRNKLFFWGGYEYMWQQPAGVPILYNVPTPAQLSGD
                     FSNAGVPSGVLSSGNSYAYRTIDQTSNLPPGASPTYIPPADFDPNIMALVKAGAYPKP
                     NLTPSAVNGWNNYEYVNQTPQNRWEATGRVDYALSDNTKIFGTYAYQKEVDQHPIAIW
                     WAAPWTLPYPSPVVASTTAQDVMANFTHVFSPTTTNEVVFTFARYINPSKLSNPSAVD
                     RNKLGMNITGLFGHTTSQIPNFKGPWAAGLPNISEFSFDGTFNGGNTFGALKRDPAIY
                     DNFTKVVGQHTFKAGFYWDTSENIQSDSDGNAQDNGTYNIGWGQYGTGNVVADLLLGR
                     LSNGGQGTGSGYTQKSAIPVLDLKFHQWSIWGQDSFKAAKNLTLNYGLRFDHLGQWYG
                     TRPGFQAWDPTLYDNTPNAPANTGLTWHALNSRVPLSGWKSPLYYYSPRLGLAYDIFG
                     TGKTVLRMGFGIFPYQVSTQIANTAGGPLGAFVYGVSNNYASYNAVSNEKPPSSVSQS
                     SYLNGGSIAAVKEGDDRVPYTTSWNFVVSQALPWRSVFEAAYIGNKSIHEIIDGTNGK
                     YLDLDNVPLGAFFKPDPLTGQYCSPSAPSGASYQCNTTHFKPYLNYQDIFLQTHGSYA
                     KYNSLQLSWQKQSGQLYFLANYTFSKVLGIWDGQTSNGNGNGSTVDPFNLQNNYGPLA
                     YDHTHILNLSYVWNLPKPVHNNFFFAGLINGWQLSGYTTLQSGAPIQPNTGGTLNAQY
                     NLPSGQTITLPNGLQSTGINASTWLGSNAPPLLPLVTCDPRKGLKSGYYFNPNCFQVP
                     PQGQQGTYRWPYLRGPSYFGSDLGVFKNFHVRENQNIQFRVQAQNFLNHPNRQFNLTQ
                     NNQDIQLIFTALNGTNTNPNTTGKPAFTTGQRLLTFSLKYAF"
     gene            complement(7863..8963)
                     /locus_tag="VFA02_01120"
     CDS             complement(7863..8963)
                     /locus_tag="VFA02_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08475.1"
                     /translation="MFYTLVFLALFTAQIAGPDQLLQRAISEQQRGDYAAAIRDYQQL
                     LELRPDDVEARVNLGAALVHAGQFDAAIEQYQLALRQMPAADQKPVRLDLALAYYKKG
                     DFHSALPQFEAVHTADPRDGRTAVLLADTEIKLGKYTDALEMLAPLEAANEQNPDFEY
                     VLGLAMIRAGKLREGVAPLEKAAGATHSGDAWLLAGSALMQLNEFVQARRDLDAALKL
                     DPELPGIYTLAGMARDQTGDVEAAEVAFREALKRNPGDFQANLYLGTILYKRRDLSEA
                     RPYLEKALQLGPSSPLARYEMAMWKSASGDYQGAANDLAQLENDEPDWLDPHVELASL
                     YYKLHRPQDGARERQIVQRLTEKQQSEGPPKP"
     gene            complement(9000..10736)
                     /locus_tag="VFA02_01125"
     CDS             complement(9000..10736)
                     /locus_tag="VFA02_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU08476.1"
                     /translation="MSIRQPAPWTRRRLLQVVPCAVAPVFLPRHLRAAPSVSFSRFVD
                     VASSAGLTQTMYFGDPGHATYIVEVMGTGCAFFDYDNDGWMDIFIPGGRRLDGIPQGS
                     SNRLYKNNRDGTFSDVTEKAGLAGAGWAYGVCVGDYNNDGFEDLFITYYGQNRLYRNN
                     GNGTFTDVTEKAGLLEDRLRFGSGCTFVDYNRDGHLDLFVSNYVDIDLDHAPRPSLNV
                     PNCNYEGVPINCGPNGLKSPRHSLYRNNGDGTFTDVSKESGIGALQGSYGLTAVVLDA
                     DEDGWPDIFVACDSTPSFLLMNNHDGTFREEGLLRGVSLSGDGAEMGGMGVGIGDYDL
                     DGHLDIVKTHFSNQATGLYHNSGKGEFDDVTAQAGLSNERRFICWGTALLDFDNDGWP
                     DIFIAAGTVYPELERIYAKYPAKNPRMVFRNLGNGKFAELGDEAGPGVMARHMARGAA
                     FGDFDNDGDMDVLIMNMNEPPSLLRNDAPSGNHWIKVRLEGVRSNRSAIGARVLARYN
                     GKVQAQCVMSQTSYLSVNDPRLHFGLGLAKTADIEVHWPSGLIEGYKDLRADQLVNIR
                     EGAGIVKGQGLK"
     gene            complement(10761..12260)
                     /locus_tag="VFA02_01130"
     CDS             complement(10761..12260)
                     /locus_tag="VFA02_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08477.1"
                     /translation="MQGHPGVSEYSDLGAWFGDHRQFDCATMAFQKALALEPRSAKLN
                     YLLGLSLYSAGRSQDAIAPLLRSVEIDPQVVKSHLLLAAAYDQIHRNREAAQQWEAAL
                     ALDPKSSVALGGLSKTLLDGGDNTAVIDLLGSADRNPQLSLDLALAYSREGMLDDAAS
                     ELNEALRKAPSSLQLTNALVTVYVKQTRYQEATKIAAAAVKRHPRDLDAQKLYLRVLV
                     LNGDFDIARPLAARLLAKTPHDFDALYLNGILEHQAGDDIAARRHLEQAVKLDPGHYN
                     ARYNLGVVLSQSHDPAGARDQLEKAIELGAREPEVRFELSKVLRALGETDKAQKQLRL
                     YQQELKAKADRTLAAQKSGQAAQELARGNAQRATVLYREALEATPDNAMLAYKLAMAL
                     DSIGDLAAERTALEQAVKLDPDFALAQNQLGYVLSHTGDIQGAEQHFRRALQSAPEYL
                     QAWINLAATLGMESRFSEAQQAAAHALQLSPGNEEAQHLSRDLAAAARR"
     gene            complement(12453..13574)
                     /locus_tag="VFA02_01135"
     CDS             complement(12453..13574)
                     /locus_tag="VFA02_01135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZU08478.1"
                     /translation="MSIPLSRPDLTEEDVKAVLSVLRSDRLSIGPKQQEFEETFARLM
                     ELPHAVAVSSGTVGLHLALLSLGIRAGDEVIVPSFTFIAVANAIRFVGATPVFADIEL
                     TTLNLDPRKVEEAITPRTRALLVVHTFGVPADMDAILSVAHKNGLLVIEDACEALGAE
                     YQGKKVGALGDASVFAFYPNKQITTGEGGMLLTPHAEVAQRCRALRNQGRYNSDEWLQ
                     HSIVGYNYRLSEIACALGCSQMARLGSILSMRESVAKRYDRRLRQDARLVLPPLALAE
                     KRISWFVYVVRLADDFEQNHRDTIFHALASVGIGCARYFAPIHLQPAYRDMLPAQPLP
                     VTEHVGRRTLALPFHNRLSEDEMDFVCQHLLSSLDQMER"
     gene            complement(13571..14725)
                     /gene="neuC"
                     /locus_tag="VFA02_01140"
     CDS             complement(13571..14725)
                     /gene="neuC"
                     /locus_tag="VFA02_01140"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZU08479.1"
                     /translation="MKRIAVVTTSRADYSHLYWPLKDLEAHPDVDLKLIVLAAHLSPE
                     FGFTVREIERDGLPIAVKLECLLSSDSDIGMAKSIGVGILALADALGQMRPDVLLLIA
                     DRFEMLAPASVALALRIPIAHIEGGEVSEGAIDDAVRNALTKMAHVHFTSTHTARQRV
                     IGMGEEPWRVHHVGAPSLDHIRRSHLPSRRELEEALGLDLSTPPVLVAYHPVTLLRDT
                     TSETDALFQALRQLPSPLVFCYPNADAGSRKIIEHTKAFLQERGQGHLFINLPAVTYL
                     GLLRCVRLIVGNSSSGIMESGSFALPTVNVGIRQKGREHGPNVLHAGASPGSILEKIR
                     QAQTEDFLSGIQNMVNPYGDGHASERIVRVLTTLPSPETLLLKRPALETP"
     gene            complement(14725..15795)
                     /locus_tag="VFA02_01145"
     CDS             complement(14725..15795)
                     /locus_tag="VFA02_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZU08480.1"
                     /translation="MKNGPYCWQEFLGRPVISIDKEAVLQSLAAKRILITGAGGCIGS
                     ALAKQCALANLDSIVFFDCSEKSMYELDCQLNGNHFFLVGDVCDETLLEEIFSRFRPQ
                     IIFHAAACKHVPLMEHNPFTAARINALGTLHVAQVAGRHQCEQLILLSTDKAANPCSI
                     MGASKRIAELVVLANQTSVQMKAVRLGNVLGSSGSVVPLFEKQIAAGGPVTVTHPEAA
                     RYFMTLDEASSLLISVVLEPYASTVLVPQLNGPHRIQDLARFLIQKHGRLTDVVFTHL
                     RPGEKLQESLLSAGERFDSRTDGPLRPVATPYMPEGNIAANLRHLQQAVERRDPCLLL
                     ETVRALVPEYHPSTVVLENLSQ"
     gene            15903..17288
                     /locus_tag="VFA02_01150"
     CDS             15903..17288
                     /locus_tag="VFA02_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU08481.1"
                     /translation="MHVTLLPAVEQLTLLKQGKISPLELAEEYISRILRFNPRLNAFA
                     DFDPDRIKQQARSIRPGPLSGLPVTIKASIATAGYRCEIGSTLLRGEIPTQNAVAVSR
                     LLAAGAMILGTTNCPELLMAYETDNLLYGRTRNPWDASRTAGGSSGGEAAAIAAALSA
                     GGLGSDSGGSVREPAHFSGICAFKPTPGRIPSEGHLPSCTGPFSVLGSIGPMARTMAD
                     IRVLYEVLAGYAPCDPSSAPVTPRQTTVADVSNITIGWFEDDGLAPVTAETRQAVREA
                     AQALERRGLRVKPFRPAMLEAARKLWDIFFVQCGAMFYGPLIAGREQTLSPTFQDFLA
                     IAKSRLSLTAESLLQAWSEWDGVRRQFLYEMRECPILLLPVCSIPAFRHGERAWAVEG
                     QTLEYLDAMRFTQWFNVLASPAAVVPVGRSAEDLPIGVQIAGRPYEDELVLTVAELLD
                     KEFGYCPPLLV"
     gene            complement(17264..20710)
                     /locus_tag="VFA02_01155"
     CDS             complement(17264..20710)
                     /locus_tag="VFA02_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZU08482.1"
                     /translation="MFLLAFLSFSPILVVTALAGPGDANTLTAARANNTGVALLNQQL
                     LEKALAKFTEAGKIEPSSPIPVINQGIALLYLRKLPEAEAAFLRAAKMDPGNLHIWYS
                     LGLTHMAGNNLALAARDFERAIQIDPEDADSHYYLGSVYLNLREYNNAVQQFEAALRL
                     NPLHASAQFGLASALQRLGRVMEAREHLRRFQTIMQTKIGTPFTITYGEQGSGAKALD
                     MLAPEKPVGPMIPVRFVPQFIHAATNTSASEASPDAAGAGACVLDVEGQGKKDIIALG
                     SGDRALHIYRASADGSLEEIPAERTGLALSGHATACAVGDYDNDSLPDLAVALSDRIV
                     LFHNLGHGKFADVTSTVAIAQLNHPSGLTFVDFDHDGDLDLFVTGSAISPGKGPNVLW
                     RNNGNSTFTEWTAPTGLGGKTDTVAAMLSDINNDRAVDLLVTGSGPSPILYENQREGA
                     FRAVPLIAEPGLSPSRGIYVFDFNKDGWMDVVLTHSGAPGISLWKNIKGRQFERVPLP
                     AEGILSAWGITALDIDNDGWIDLAAIVQTTSGPTLHIWRNRGPQGFEDVSAATGADKI
                     RLSNPRALIAADMDGDGAADLIVTQWNAPPVVLRNTGGSRNHSLRILLAGLNDNKSAI
                     GTKVEVFSNGQVQKFEVAGSSGYLSQGPTEIIAGLGEAAEADVVRLLWPTGVPQDEMD
                     VAANKPEKLLELDRRGSSCPVLFAWNGSKYEFVTDVIGAAVIGHWISPTSTNHPDPDE
                     WIKVDGSQLRPHHGFLSLRFGEPMEEINYIDQLRLVAVDHPANTEVYPDERFLNGPPF
                     ASGKTVIAGPLTRLPSGAWDDHGHDVLTLLARADHQYVRDFTTLHYAGYANMHSLTLD
                     LGSWTSRNPLRLFMRGYIEYFSASSMYAAWQAGLRPVSPYMEAQMPDGSWKRVLDDMG
                     FPAGLPRTIVVNLTHRLPEGVHRIRIRTNLQIYWDQVLVDNGPDMSGQARETEAPLAS
                     AHLAFRGYPQQIEGATPGDLTYRYDRISTTGPFQWQRGDYTHYGDVTVLLQRVDNQYV
                     IFGSGEDIDAEFNASLLPPLPSGWKRDYFFYANGFVKDMDFYEAMPFTVAAMPFHEMS
                     TYPYPQTEHYPEDNRSVRYQLDWDDRFESGERKQLYQFQYGPEFSKPILPAASQTSRG
                     GQ"
     gene            21033..>22681
                     /locus_tag="VFA02_01160"
     CDS             21033..>22681
                     /locus_tag="VFA02_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZU08483.1"
                     /translation="MNTFVRRSGLFFLCLVPSLSLAQMPGQKIPGQPTITTAQTSSGL
                     RATIGKEQMEITVCGDSVIHVVATPDPEAKPSPKPWMLDHAQSCPGATFRYSQHDGRA
                     TLETSRLKVELSLDRGNVVFQTAAGETLLREGGSVPRTYEPAELNGEQTFHITDRFSP
                     NVTEAFYGLGQHQSGMFNYRGSTVELGQNNTDVAIPLLVSSNGYAILWNTAALTYVDN
                     RFPSELAFRSLAGHAVDYYFIYGPEMDSIIHQYRSMTGHTPMLPKWAYGLFQSKDRYV
                     SLDEILKIAGRYRAEHIPLDAMVQDWFWWKHEGDPVFNENYHDVPGDLKKLHQMNVHA
                     MISVWGLFDPASENFKELTENHFDVPGAHVYDATNPAARDFYWNKLAGKLFAQGWDAF
                     WLDSAEPEEYWPHMGDAILQNKRLYIGNGAEFTNIFPFMHTTGVQQHWKETTDRKRVF
                     LLTRSAFLGQQRVGATVWSGDVYTTYWALSHQVPAGLNFALSGYPYWTTDIGGYWPHE
                     NDVIHDPAYQELYARWFEFGTFCPVFRTHGHRAENEMWAYD"
BASE COUNT         5258 a         6037 c         6170 g         5216 t
ORIGIN      
        1 tacagcaatg ttccatttag ttggggtaag gctcccctgg gcgcacaatt ctgatctgat
       61 gatcggtgaa actcagcaag ccattcggaa gctggacttt aggcatatga cagcttacgc
      121 actttgaact ggagaccgga caactcctga tatgggccgt ccttgcctct ggagtatgac
      181 attcgaggca ttttgcgtca tagtaggacc catcatgcga caatggccca tgcggatcat
      241 ggcaagcgat gcaactgata cgcggatcat ttccgttgaa gcaccggctg ttggccaagc
      301 gatatggctg aaaacggaca tccactgggc cgcgctcccg gctcctgatg accgcttccc
      361 atgtgcgatg acactgcccg caaaaattag aaatgtcttc cgaggacata ctgccgaggt
      421 ccggtggaat agaggatctg ttgccctgca tcatgtcatg ggaatgcgcc tctgctccct
      481 gatggcaccg ctcgcaggtt atgcctggat gcacgccatc cagggccagt ctgtcttcat
      541 gaatggcatt ggtggcgtgg caatggaagc atgtcatggc ctcagcagga gaaagctcgc
      601 ggccaaatgc ttcctccaaa tttcgtggag tgagcttttc gtctccggtg gtgatattga
      661 gtccatcggt tgaaagatag taacttacca ggctttcgta aaatttgcca ttacgctcaa
      721 tgacccaggt ctgattgcct gcgcctaagg cccacttcac aggcaaagaa atgctctgtg
      781 cgccatgact gacagtgtat gtaacgtgcc cttctctggc ttccagagag taggtatagc
      841 cgttctgctg aaaaatcagc tttgggtggg ttctgaataa aagatccaat ggcgccagtt
      901 gcatcgcgtt tgccatggga gtccgcggct gcgcccatgc ttcacgcgga tgacagagcg
      961 cacattgctg ctgtgctggc aaggagaccg gtagcagaag gacgatccat tgcagaaggt
     1021 cacggagtct catgttgctc tccggcagtg gcatgcaggc accacaggcg ttgctggaag
     1081 gcatcttcac tgcggccaga ttttgaatat gcctttgcca atgcaatatg gacttcaagg
     1141 ttgtccgggg ccatcttcag ggcctcttca agatgtactg tgcccgcgct gatgtttccg
     1201 gtctccacca gagcttttcc aaggatcaga tgtgggagca tttgcgatgg caattcctcg
     1261 atggcctctt gtgcgtaagg gagcgccatc ctgggttcgt tctgtatcag ataggcccag
     1321 gccagtatgg ccgcgcaact ggcattggca gggtagtgct gcatctcctg gcggaactgt
     1381 tttatggcag cgtccggatc cgatggaaaa agcagatacc cgtaaagata atgcacattt
     1441 tcggcatcgt gggatccttg aaacaggtcc tgaaacgcct gctgcgcccc agcggtattc
     1501 ccggtcataa aaatccacgt ggcccttccg gctgccagaa agaggtcttg ctttgctgca
     1561 ggaacatctt tgggaaggag gggcattcgc aaccctgcca gccctatggc cagaaaaata
     1621 tccggaccag agatgccatt ttgagcaaag aatgtgaaac ggtccattgc ttcttcaaat
     1681 ctgccggaga gggtaagcaa aatggcttca tgatagcgca gaatgcgtgt gttttccgga
     1741 cggttggctg cacccagtga gagtccgcgc tgcaggtcag cgagagactg ggcgtagtcg
     1801 ccggtttcat attcgcaaag gccacgcagt gcaaaggcag gcccggccgt aggattgttc
     1861 gcaataaagt gggtcagcgc gttgcgggca tgggcataat cattgtttgc gtattggagg
     1921 gagcccagat accaccatcc ttcggcccat ttcgggttta actccacagc ctgctgataa
     1981 agagagattg catccgttgc atcatgctgt tctctggcct gcgctgcttt tgccaccagc
     2041 tcatcgaagg aaaggccatt ctgcgcacgc gctgttacag cactgcacaa caaaagcatg
     2101 accatccata aaccaaagtg tttgcccagc attacttttg catctccacg acagtgagga
     2161 cctgatcggc agcaatatcg tgaagtgtct gcacgatccc tgagggccaa tgtatctcaa
     2221 cgaggccgac acgcgtgtca gccccaagcc cgaaatgcac tggtccatcg ctggaggaag
     2281 catagcccac gctggtggtc atgtggttga attgaatgcc gctcttcgaa acaatcttaa
     2341 tgctggcgcc gatgccatcg cgattgctct tcacacccct aagtgcaata tcaagccaat
     2401 gaccagaatg cgggctacgg ttcatccata tctccgcctg ctgcccgagt gccgttgcta
     2461 ttacgtccac gcgcccgtct ccgtcaaaat cgccaaaggc acagccacgg tgacgggcag
     2521 gaggagcagc cgtcagtcct gcctcagcag taaacgcttc ccattttcca ctggggccaa
     2581 ggttacgaaa caccgtattg ggctggtcaa tttctgtccc cggaagtggc tgcgattcta
     2641 catgtccgcg cgtcacgaac aggtctttcc atccatcatt gtcaaaatcg tataggccag
     2701 cgccataccc ggccatggat gcgctcagca cacgcattcc cgatgcattg gtcacttcct
     2761 ggaagtctcc ctttccggtg ttctgaaaga ctgggaaggt ctggttattc agagcgacaa
     2821 agacgatgtc tggccaacca tcgttattgt agtcgcgaaa gtcgaggccc attcccgaaa
     2881 tgaatgcccc atcttcagcc agcgccaccc cagtctgaaa ggcaacttcc tcgaatttgc
     2941 ttcccaggtt atgaaagaga aaattatatt cggcatcatt ggtcacaaaa agatcagaac
     3001 ggccatcgtg atcataatct gccacaccaa cgcccatccc tttgcctacg tggtcgcgga
     3061 tgccccattc ttttgagaca tcgcgaaatg tgccattacc gttgttgagg aaaagctgac
     3121 tgggctggcc cttgtaatag cgcggatgac agtaatcagc cacgcctcga aaactgcaga
     3181 gcggctggct ggaatacttc cactgcatat agttgactac gaagaggtcc agcaggccgt
     3241 cattgtttac gtctacccag gctgcggcca cagaccagag cggcccatac tgagggtcaa
     3301 ttgatttatc caggccggcc tttagcgtga catcggtgaa ggtcccgtca caattgttgt
     3361 gatagagagt gtggtggaaa acgccggcaa tgaagatgtc tgtacaaccg tcgttatcgt
     3421 aatcagcaac agcaacgccg atatcgtagc cggatccttc cagaccggcc tgctttgtta
     3481 catcagtgaa gacactgtta ccgtcgttgc gaaaaaggtg gttgctgtac ttaggagaat
     3541 ctttttttaa agtggcgatg ttggcgccat tggtaaagaa gatatcgggc ctgccatcat
     3601 ggttgtaatc gaaaacagcg acgcccccgg ccatgctttc aggagcattt ctgcccgggg
     3661 tctcgtcgtt ctcaaggcga aaggggatgc gcttcagatc gaagcgtatc aggccctgct
     3721 gcggtctatt ctgagaaaga agcacacacg aaacaagtgc gaaaacccat gcaggcaaaa
     3781 gtgcaaattt cagagggcgc atcttctccc tatatagaag agaaggggtg gcaaaaccca
     3841 ccccaattag agaggcgata atttaaaaat tcagaaagca tacttcagtg agaaggtcaa
     3901 taaacgctgt ccggtcgtga aagccggctt acctgttgtg ttcgggttcg tatttgttcc
     3961 attgagtgca gtaaagatca gctggatgtc ctggttgttc tgagtcaggt tgaattgacg
     4021 atttgggtgg ttcaggaaat tctgggcctg aacccggaac tggatgttct ggttttcccg
     4081 gacatgaaag tttttaaaca cacctaaatc gcttccaaag taagatgggc ctcttaaata
     4141 aggccaacga tatgttccct gctggccttg cggaggcacc tggaagcagt tcgggttgaa
     4201 ataataacca gacttaaggc cctttcttgg atcacaagtt accaatggca acagaggcgg
     4261 tgcattagaa cccagccagg tagatgcatt gatacctgta gattgcaatc cgtttggcag
     4321 tgttatcgtc tggccggacg gcaggttata ttgagcgttc aaagtaccac ccgtgttggg
     4381 ttgtatagga gcgccactct gtaaggttgt atatccggaa agctgccagc catttataag
     4441 cccggcaaag aagaaattgt tatggaccgg tttgggcaga ttccacacat agctcaaatt
     4501 cagaatatgt gtgtggtcat atgccagagg cccataattg ttctgcaggt tgaatggatc
     4561 cacagtagat ccattaccat taccattact ggtttgcccg tcccagatac ccaatacctt
     4621 gctaaaggta taatttgcca agaagtagag ctgtcctgat tgcttctgcc aggatagctg
     4681 cagagagttg tatttagcat agcttccatg ggtctgcaaa aagatgtctt gatagttcaa
     4741 ataaggtttg aaatgtgtcg tgttgcactg ataacttgct ccgcttggtg cgctagggga
     4801 gcaatattgc cctgtaagcg gatccggctt aaagaatgcc cctaaaggaa cattatcgag
     4861 atccagatat ttcccgtttg tcccatcaat gatttcatgg atgcttttgt tcccgatgta
     4921 agccgcttca aagacagagc gccacggaag tgcttgtgac accacaaagt tccaactggt
     4981 agtgtatgga acacgatcat cgccttcctt aacggcagcg atacttccac cgttcaggta
     5041 agatgactga ctgacagagg aaggtggttt ttcattggat acagcgttat aacttgcgta
     5101 attgttagat acgccataga caaatgcgcc caaggggcca cccgccgtgt tcgctatttg
     5161 tgtggaaacc tggtatggga agatgccaaa acccatgcgg agaactgttt tgcctgttcc
     5221 gaagatatcg taggccaagc ctaggcgcgg cgaatagtaa tagagcggcg atttccaccc
     5281 tgataagggt acccggctgt taagcgcatg ccaggtgaga cccgtatttg ctggcgcatt
     5341 tggtgtattg tcatacaatg tgggatccca agcctgaaaa cctggccttg taccatacca
     5401 ctgcccgaga tggtcaaatc tcaatccgta attcagagta agattctttg ccgctttaaa
     5461 ggaatcctgt ccccagatag accattgatg gaatttcaaa tccagaaccg gaatcgcgct
     5521 cttctgggta taccctgaac ctgtaccttg ccccccattt gacaatcgtc ctaagagaag
     5581 atctgcgacg acatttcctg ttccatactg tccccagccg atgttatatg tgccattgtc
     5641 ctgtgcattg ccatcgctgt cggattgaat attttcgctg gtatcccagt agaatccagc
     5701 tttaaaggtg tgttgtccca caacctttgt aaagttgtca taaattgccg ggtccctttt
     5761 aagggcacca aaagtatttc cgccattaaa ggtgccgtcg aaagaaaatt cggaaatgtt
     5821 aggcaatcct gcagcccaag gtcccttaaa atttggaatt tgcgaagttg tatgaccgaa
     5881 caggccggtg atgttcatcc caagtttatt gcgatcaaca gcagacgggt tggacaactt
     5941 gcttgggtta atatagcggg caaatgtgaa aacaacttcg ttcgtggtgg ttggactgaa
     6001 cacgtgagta aagttggcca tgacatcctg tgctgtagtg cttgctacaa ctggagacgg
     6061 atagggtaat gtccagggag cagcccacca gatggcgatt gggtgctggt ctacttcctt
     6121 ctggtaggca tacgtaccaa atatcttggt gttgtcgctc aaggcatagt ccacccggcc
     6181 ggtggcctcc cagcggtttt gtggcgtctg gttcacgtat tcatagttgt tccagccatt
     6241 cactgccgaa ggagtcagat tgggcttcgg ataagcccct gcctttacaa gggccatgat
     6301 attgggatca aaatcggctg gtggaatgta cgttggtgaa gcaccggggg gaagattgct
     6361 agtctgatct attgttctat atgcatagct attaccactg gacaacacac ctgaggggac
     6421 gcccgcattg ctgaaatcgc cggaaagctg tgccggagta gggacgttgt aaagaatggg
     6481 aacaccagcg ggctgttgcc acatatattc atagcccccc cagaagaaca acttattcct
     6541 gttcttatta aaacccgtaa aaggaaagag cacgggtcca ccaatattgc cacccaaata
     6601 atagtaatgc tcatcaggga gggcctgctt cagtgcggta gacttagcct gagatgcgct
     6661 catgccattg gcaatgtagt tcttaatatt acctgcaatc tgacttttag cataagattc
     6721 aatggaattg aatgtgctgt ttcgagcata gaaataacct tccccatgga atgcctggcc
     6781 gccgtttttg ctgaatgcct ggaagatgac ggggcctttg gcaaactcag cgccatagga
     6841 agatgtcagt attttgattt cggaggtcat gtcctggttg atgttggcta tctgtgtgcc
     6901 cgcatttcca gggtctacca gattggcgcc gtccagcata aaggccatcg atccgtaggg
     6961 ttgggttcca ttcagcgaat atgcgccaac cgggccattg tttgtaccag ttatctttgg
     7021 attgaagctg gatccttgcg tcaaaccatt ggtgaatgct gcgcctggta ttaatttcag
     7081 cagctctccg gcatctcggc caccaagcgt catgtcctga atcatttctg tacttaatgt
     7141 cgtgctgact tcgcccgtat ccatggggat gactgcagct tcactggaaa ctacctgtac
     7201 agaggtagag ctagatccaa ctttgagtgc aatattcggc aggcttcggt tgtcgccctg
     7261 gttcatcaca acaccctgct cttgccaagt ggtaaaaccc gtggcagaga tcgtaatagt
     7321 gtaagtacct ggaggaacgg cagtaattgt aaaaacacct gcattgttcg acttggaaac
     7381 acgcaaatca ccactactct ggtttctaag ctcaatagcg gcattcggaa caactgcgcc
     7441 ggactggtca tagacaatgc cgctgatggt accagtcaac tgctgcgcgc ggactgacgg
     7501 caggaggaac cctgacgtgg ccatcgccag cagcacccaa agaaattgtt ttctgaattt
     7561 tcctgttgta ttccgaaagc aagcagacaa aaccctgctg aattccgcca tggtcctaac
     7621 ctcctgaatt aaaagccatt tgcatgaacc agaagtctgg tttgcagatg gtaccgttat
     7681 cattgagctt tagtaactcg cctgtcaata aagcgtccaa gttctctgaa tattattatg
     7741 gggggcaact tttaaaacgc tttattagga gcctataaat acgcgaacca aaatacgcct
     7801 gtcaatagaa atttaaagca aggtttctga taacgctatc atattgaaaa aataaattcc
     7861 ctttaaggct ttggcggccc ctcggactgc tgcttttctg tgaggcgctg gacaatctgg
     7921 cgctcccggg cgccatcctg gggccgatgc agcttgtagt agagcgatgc cagctcaaca
     7981 tgagggtcga gccagtcagg ctcatcgttt tccaactgtg caagatcgtt ggccgctccc
     8041 tggtagtcgc cggacgcgct cttccacatt gccatctcgt agcgagcgag gggagaggaa
     8101 ggccccagtt gcagtgcttt ttccagataa ggtcgggctt ccgacagatc gcgccgttta
     8161 taaagaatcg tcccgaggta aagatttgcc tgaaaatcac cgggattgcg tttcaatgcc
     8221 tcgcggaagg ccacctctgc tgcttccaca tctcctgtct gatctcgcgc catcccagcc
     8281 agggtgtaga ttccaggcaa ctccgggtcc agtttcagag cggcgtccag gtcacggcgc
     8341 gcctgtacga attcgttgag ctgcatgagc gctgaacccg ccagcagcca ggcatctccg
     8401 ctgtgcgttg cgcctgcagc cttttccaga ggggcaaccc cttcgcgcag cttccccgcc
     8461 cgtatcattg ccagacccag aacatactca aaatcaggat tttgttcatt tgccgcctcg
     8521 agcggcgcga gcatttccaa cgcgtcggta tattttccca gcttgatctc ggtatcggcc
     8581 agcaggacag cggttcgtcc atcccgggga tccgctgtat ggacagcctc gaattgaggc
     8641 agcgcgctat ggaagtctcc ctttttgtag tatgccaacg ccaggtccaa gcgcacaggt
     8701 ttctggtctg cggcaggcat ctggcgcaag gccaactgat actgctcgat ggcggcatca
     8761 aattgacctg catggaccag ggccgcgccc agattgacgc gtgcctcaac atcatccggg
     8821 cgcagttcaa gcaattgctg atagtcccgg atcgccgcag catagtcgcc cctctgctgt
     8881 tctgagatag cgcgctgcaa aagctggtcc gggcctgcga tttgtgccgt gaacaaagcc
     8941 aggaaaacga gcgtgtaaaa catggcctac agcatagccc attcagcctg cgaagtgatt
     9001 tatttcagtc cttgcccttt cacaattcca gcgccctcgc ggatgttcac aagctggtca
     9061 gcgcgcaggt ctttgtaacc ctcaatgaga ccagaaggcc agtggacttc tatgtctgct
     9121 gtttttgcca gccccagacc aaagtgcaga cgtggatcgt tcactgagag atagcttgtc
     9181 tgactcataa cacattgtgc ctgtactttg ccgttataac gggccagtac gcgtgcgcca
     9241 atagcactgc gattgcttct caccccttcc agtctgacct tgatccagtg attgccggaa
     9301 ggagcatcat ttcgcagcaa agacggaggc tcattcatgt tcatgatgag tacgtccata
     9361 tcgccatcgt tgtcgaagtc gccaaaagca gcgccccggg ccatgtgtct ggccatcaca
     9421 ccaggtccgg cttcatcgcc aagttctgca aactttccgt tgcccaggtt tcgaaagacc
     9481 atacgtggat ttttagctgg atatttggca taaatacgct cgagttccgg atagaccgtc
     9541 cctgcagcaa taaaaatgtc tggccagcca tcgttatcaa agtccagtaa tgcggtcccc
     9601 cagcagataa accgccgctc gtttgaaagg cccgcctgcg cagtgacatc atcaaactca
     9661 cctttcccgc tattgtgata cagtccggtg gcctggttgg agaagtgtgt ctttactatg
     9721 tccaggtggc catcgagatc gtaatctcct atgccgaccc ccattcctcc catctccgcg
     9781 ccatcgccgc tgagcgacac tccgcgcaac aaaccttcct cgcggaaggt gccatcatgg
     9841 ttattcatta agaggaaact cggtgtcgaa tcacaagcaa caaagatgtc tggccaacca
     9901 tcctcgtcag catcgagtac aacggccgtg agtccgtaag agccctgcaa ggcgccaatc
     9961 ccggactctt tcgagacgtc agtgaatgtc ccgtcgccat tgtttcgata aagtgagtgc
    10021 cggggagact tcagcccatt tgggccgcag ttgatgggta ccccttcata attacagttg
    10081 ggcacattca gagagggcct cggcgcgtgg tccaggtcaa tgtccacata attggagacg
    10141 aaaaggtcca agtggccatc gcgattatag tccacaaaag tacagccaga cccgaagcgg
    10201 aggcggtctt caagcagacc tgccttctcg gtcacatcag taaaggtgcc attgccgtta
    10261 ttccggtaga gcctgttttg gccataatac gtgataaaga gatcttcaaa cccgtcattg
    10321 ttgtaatcgc ctacacaaac accgtaggcc catcctgcac ctgccagacc tgccttctcg
    10381 gtcacatcag aaaacgtgcc atcacggttg ttcttatata gccggttact ggagccttgg
    10441 ggaatgccat ccagccgccg cccgccggga atgaagatat ccatccagcc atcgttgtca
    10501 taatcaaaga aggcacagcc agtgcccatg acctcaacga tgtaggtagc gtgtccggga
    10561 tcgccaaaat acatcgtctg tgtaagcccg gcactggacg caacatctac aaagcgggaa
    10621 aacgacaccg aaggtgcagc ccgaagatga cgtggtaaaa atactggagc gactgcgcaa
    10681 gggacgacct gcaacaatcg tcgcctcgtc cagggcgctg gttgccgaat gctcatatct
    10741 ggaacctact atacggcgaa tcaacgccgt gccgcagcag ccagatcgcg gctcaggtgc
    10801 tgcgcttctt catttccagg actgagctgc aatgcatgcg ctgcggcctg ctgcgcttca
    10861 gaaaagcgcg attccatgcc cagcgttgcg gccagattga tccatgcctg caggtattca
    10921 ggcgctgact gcaatgccct gcgaaaatgc tgttctgctc cctgaatatc tcctgtatgt
    10981 gacagcacat aacctaactg gttctgcgcc aaggcaaagt ctggatcgag tttcaccgcc
    11041 tgctcaaggg ccgtgcgctc tgctgccagg tctcctatag agtccaaggc cattgccaat
    11101 ttatatgcaa gcattgcgtt gtccggagtg gcctccaatg cctcccgata aagtacggtt
    11161 gccctttgcg cattgcctcg ggcaagctcc tgcgcagcct gtccggattt ttgtgcagca
    11221 agggtgcggt ctgccttggc ctttaactcc tgttgataga gtctaagctg tttctgggcc
    11281 ttatcggttt cacccagtgc acgcagcact ttggacagtt cgaaccggac ctctggttct
    11341 cttgccccca gttcaatggc cttttccagt tggtcgcggg cgcctgcagg gtcgtgcgac
    11401 tgggacagta cgacgccgag attgtagcgc gcgttatagt ggcccggatc gagcttgacc
    11461 gcctgttcaa gatgcctgcg tgcggcaata tcatcaccgg cctggtgctc cagaatgccg
    11521 ttcagataca gcgcgtcaaa atcatggggg gtctttgcaa ggagccgggc agcaagcggg
    11581 cgcgctatat caaaatcgcc gttcagtacc agcactcgca gataaagctt ctgcgcatca
    11641 aggtctcttg gatgccgctt cactgccgca gccgctattt ttgtggcctc ctgatagcgc
    11701 gtctgtttca cataaaccgt aaccagagca tttgtcagct gcagagaaga tggggcctta
    11761 cgcaatgcct cattcagctc gctggcggca tcatccagca ttccttccct gctataagcc
    11821 aacgccaggt ccagtgagag ttgcggattg cggtctgcgg aacccagtag atcaattaca
    11881 gcggtattgt ctccaccgtc cagcaacgtt ttgctcaggc cgccgagggc gacggaagat
    11941 ttgggatcaa gggcaagcgc ggcctcccac tgctgagcag cctcacggtt gcgatggatc
    12001 tggtcatagg ctgccgccag cagcaaatgc gatttcacga cctgaggatc aatctcgaca
    12061 gagcgtaaca atggggcgat ggcgtcctgt gaacggccgg ctgaatacaa actcaggcca
    12121 agcaaataat tcagctttgc tgagcgcggt tccaaggcca gggccttctg gaaagccata
    12181 gtcgcacagt caaactgacg atggtcgccg aaccaggcgc ccaaatcact gtattctgac
    12241 actcccggat ggccctgcat cttgacctgc aatgcttgcg gaacagaaca ccttgtttgc
    12301 gcatatacaa atgcatgcag taggaacgca caaaaaagac acgctttggt ggacattaca
    12361 gacaggccgt atttgtaaga ttactgcccg gacagatctc aggcaatccc tgaggtgcct
    12421 ggaacaatac gacgggcctc gagcgaagcg tatcagcgtt ccatctggtc cagcgatgag
    12481 aggagatgtt ggcaaacaaa atccatctca tcttcagata accggttgtg aaagggtaaa
    12541 gcgagtgtcc gtcggccaac atgctcggtg acgggaagcg gctgtgcggg cagcatatcg
    12601 cggtaggccg gttgcaaatg gatcggagcg aaataccggg cacagccaat gcccacagaa
    12661 gccagagcat ggaaaattgt atcgcgatga ttttgctcaa agtcatcagc cagccggaca
    12721 acatacacaa accaactgat gcgcttttcc gcaagggcga gtggaggtag aaccagccgc
    12781 gcatcctggc gcaatcgccg gtcatatctt ttcgcaaccg attcacgcat agagaggatg
    12841 cttcccaggc gcgccatctg gctgcaaccc agagcgcagg cgatttcaga aaggcgatag
    12901 ttataaccga cgatgctgtg ctggagccat tcatcggaat tgtaacggcc ctggttacgc
    12961 aaggcccggc atcgttgggc cacctctgca tgtggggtca ggagcattcc accttcgcct
    13021 gtggtgatct gcttgttggg gtaaaaggca aagacggaag catccccaag tgcgccaact
    13081 tttttccctt ggtattcagc gccgagagcc tcacaggcat cttcaatgac gagcaggcca
    13141 tttttgtgcg caacggagag aatcgcgtcc atatcagccg gaacgccgaa ggtatggaca
    13201 acaagaaggg cgcgcgttct gggcgtgatg gcctcttcta ctttcctcgg gtcaagattg
    13261 agggtcgtta gctcaatgtc ggcaaagaca ggtgtggcac cgacaaaacg aatagcattg
    13321 gctacggcaa taaaggtgaa ggaaggcacg atgacctcgt cccccgctct aattccaaga
    13381 gacagaaggg ccaggtgaag tccgaccgtt cccgagctga cagccaccgc atggggcagc
    13441 tccatcaacc gtgcgaaggt ctcttcaaac tcctgctgtt tggggccgat actcagccgg
    13501 tcgctacgca ggacgctgag gacggccttc acatcctctt ctgtcagatc tggccgcgaa
    13561 agaggaatgc tcatggtgtc tccaaagcag ggcgttttaa cagcagcgtt tctggggatg
    13621 gcagagtggt aagcacacgg acgatacgct cagatgcgtg gccatctccg tatgggttga
    13681 ccatgttttg gataccagac agaaaatctt ctgtctgtgc ctggcggatt ttttcaagaa
    13741 tggatcccgg ggaggctccc gcatgaagaa cgttcggtcc atgctcgcgt cccttttgcc
    13801 gaatgccaac gttgactgtg ggcaaagcaa aggagccgga ttccatgatg ccgctgctgg
    13861 aatttccaac tataagacgg acgcaacgca gaagccccaa gtatgttacc gcaggcagat
    13921 tgatgaagag atgcccctgg ccccgttcct gaagaaatgc ctttgtgtgc tcaatgatct
    13981 tgcggctgcc tgcatcagca ttggggtagc agaagaccag ggggctgggt aactggcgga
    14041 gcgcctggaa cagagcatcg gtttcactgg ttgtgtcgcg cagcagagtc acgggatgat
    14101 aagccacgag taccggcggg gtgctcaggt cgaggccgag tgcttcttca agttctctac
    14161 gggaaggcag atggctgcgg cggatgtggt ccagcgaagg tgcacctaca tgatgtacgc
    14221 gccatggctc ctctcccatg ccgataacac gctgacgcgc cgtatgggtc gaagtaaaat
    14281 gaacatgggc cattttggtg agggcgttgc gcacggcatc gtcaatggca ccttcgctca
    14341 cttctccgcc ttcgatatgc gctatgggaa tgcgaagggc cagcgcaacc gacgcagggg
    14401 ccagcatctc aaagcggtct gcaatcagca aaagcacatc gggacgcatc tgcccaagcg
    14461 catccgctag cgccagaatg ccgacgccaa tactcttggc catgccgatg tccgaatcag
    14521 agcttaacaa gcactcgagt ttcacagcga tgggcaagcc gtcgcgttca atttcgcgga
    14581 ctgtgaaacc aaattcaggc gagagatgcg ctgccagcac aatcagcttc aggtccacat
    14641 caggatgcgc ctcaagatct ttcagcggcc aatagaggtg gctgtagtcg gcacgggagg
    14701 tggtcacaac ggcgatgcgt ttcattactg tgaaagattt tccaatacaa ctgtactggg
    14761 atggtattcc gggacgagag cacggaccgt ctcaagcagc agacaaggat ctcgtctttc
    14821 cacggcctgc tggagatggc gcagatttgc ggcgatattt ccttcgggca tatagggcgt
    14881 ggccactggc cggagagggc catcggttct gctgtcgaat ctttcgccag ccgaaagcag
    14941 agactcctga agtttttccc cgggacgcag atgggtaaat acaacgtcag ttaacctgcc
    15001 atgtttttgt ataaggaacc gcgcgaggtc ctggattcga tgcggcccat tgagttgcgg
    15061 caccaggact gtcgaagcat acggctcaag gactacgctg attaacagcg aggaggcctc
    15121 atccagggtc ataaagtaac gcgcggcttc aggatgcgtt accgtcacag ggccacctgc
    15181 ggcaatctgt ttttcaaaca atggcacaac acttcctgag gacccaagaa cattccccag
    15241 gcggacggcc ttcatctgta cgctggtttg gtttgcaagc acaacgagtt cggcgatccg
    15301 tttgcttgct cccatgatgc tgcatggatt cgcggccttg tctgtggaaa gaaggatgag
    15361 ctgttcacac tggtggcggc ccgctacctg ggccacatga agagttccga gggcatttat
    15421 acgtgcagca gtaaaaggat tgtgttccat cagagggaca tgcttgcagg cagcagcgtg
    15481 gaagatgatc tggggtcgga agcgggagaa gatttcctca agcaatgtct catcgcacac
    15541 atctcccact aaaaagaaat ggttgccatt aagctggcag tcaagttcat acatgctctt
    15601 ctctgagcaa tcaaaaaaaa caatcgaatc caggtttgca agagcacact gctttgccaa
    15661 agctgaaccg atgcagcccc cggccccggt gataaggatg cgtttggcag caagagactg
    15721 gagcactgcc tccttatcta tagaaatgac tggccttccc aggaactctt gccagcaata
    15781 tggaccgttc ttcatgcttt gcgtcagaaa cactccctca cagccatcga aaacacaaat
    15841 tccagtgccc tatactggat tttatcggcc ctctgtccag tgacgctccc aggagaagat
    15901 ggatgcatgt aaccttactt cctgctgtcg agcagttgac gctgctgaag cagggaaaga
    15961 tttctcctct tgaactggcg gaggaataca tctcacgcat tttgcgattc aaccccaggc
    16021 tgaatgcctt tgctgatttt gatccggacc ggataaagca gcaggcgcgc tccatcagac
    16081 caggaccact cagcggacta cctgttacca tcaaggcctc cattgcaacc gccgggtacc
    16141 gttgtgagat tggcagcact ttgcttcgcg gagagatccc aacgcaaaac gccgtggccg
    16201 tgagcaggtt gctggctgct ggggccatga ttctgggaac caccaattgc cctgaactgt
    16261 tgatggccta cgagacagac aaccttttgt atgggaggac gagaaacccc tgggacgctt
    16321 cgcgtacggc tggaggatcc agcggcggag aagcagccgc cattgccgca gctctttccg
    16381 caggaggtct gggcagcgat agcgggggct ctgtgcggga gccggcccat ttttctggta
    16441 tctgtgcatt caaacccaca cctggacgca ttccctccga aggacacctg ccgtcgtgca
    16501 ccggaccgtt ttccgtcctg gggtccatag gcccaatggc gcgcacgatg gcagatattc
    16561 gtgtgttgta tgaggtactg gctggctatg ccccctgcga tccatccagt gctccggtga
    16621 cgccgaggca gacaacggtc gcagacgtca gcaacatcac catcggatgg tttgaagacg
    16681 atggtctggc gccggtaaca gcagagaccc ggcaggccgt acgcgaggct gcgcaggcgc
    16741 tggaacgccg cgggcttcgg gtgaagccgt ttcgccccgc catgctggag gcagcgcgaa
    16801 agctgtggga catttttttc gtacaatgcg gagcaatgtt ctacggacca ctgattgcag
    16861 gccgcgaaca gacactcagc ccgacattcc aggatttcct ggctattgca aaatctaggc
    16921 tctccctcac ggcggagtcc ctgttacaag cctggtcaga gtgggacggg gtccgcagac
    16981 agtttcttta tgaaatgaga gagtgcccaa tcctgttgtt gccggtttgt tcgattcccg
    17041 cctttcggca tggagaaaga gcatgggcag tcgagggaca aacgctcgaa tatctcgatg
    17101 cgatgcgatt tacgcagtgg tttaatgtgc tggcttcacc ggctgcggtt gtccccgtgg
    17161 gccgatcagc agaggacctt cccatcgggg tgcagattgc gggaaggccc tatgaggatg
    17221 agctggtgct gaccgtcgca gagcttctgg acaaagaatt tggctactgt ccccctctgc
    17281 ttgtctgact tgcggcagga agaatcggtt tagaaaactc aggcccgtat tgaaactgat
    17341 aaagttgttt tcgctcgccg gattcaaagc ggtcgtccca atccaactga tagcgtactg
    17401 agcggttgtc ttccgggtag tgctcggttt ggggataggg ataggtgctc atttcatgaa
    17461 atggcatggc agcaacggta aatggcatgg cctcgtagaa atccatgtct tttacaaaac
    17521 cattggcata gaaaaagtag tcgcgcttcc agccggatgg aagcggaggc agcaaggacg
    17581 cgttgaattc tgcgtcaatg tcttctccgc tgccaaagat gacgtactga ttatcaacgc
    17641 gctgtagcag cacagtcaca tctccatagt gggtgtaatc gccacgctgc cactgaaaag
    17701 gcccggtggt gctgatgcga tcgtagcgat aggtcaggtc accgggagta gcgccctcta
    17761 tctgctgcgg gtatccgcga aaagcgagat gggcggaagc cagaggtgct tctgtttccc
    17821 gtgcctgtcc ggacatgtca gggccattgt ccacaagcac ctggtcccag taaatctgaa
    17881 ggttggttcg gatacggata cgatgcacgc cttccgggag acgatgggtg aggttcacaa
    17941 caatggtacg ggggagacct gccggaaagc ccatatcatc cagaacacgc ttccagctac
    18001 cgtccggcat ctgtgcttcc atatagggcg aaacagggcg caaacccgcc tgccatgcgg
    18061 catacatgga actggcgctg aagtattcaa tgtaaccgcg cataaaaaga cgcaatggat
    18121 ttcgtgaggt ccagctgccc agatccaggg tcaggctgtg catattggca tagccagcgt
    18181 agtggagtgt tgtaaagtcc cgtacatact gatggtctgc acgggccagg agcgtgagca
    18241 catcgtggcc atggtcgtcc catgctcctg atggaagacg ggtgagcggg ccggcaataa
    18301 cggtctttcc tgaggcaaaa ggaggaccgt tgagaaagcg ctcatccgga taaacttcag
    18361 tattggcggg atgatccacg gcaaccaggc gaagctggtc tatatagtta atctcttcca
    18421 tcggctcgcc aaagcgaagg ctgaggaaac catggtgcgg gcggagctgg gagccatcca
    18481 ccttgatcca ctcatcagga tcgggatggt ttgtggatgt gggcgagatc cagtgtccga
    18541 tgaccgcagc gccgatgacg tctgtgacaa attcatattt gctgccattc catgcaaaga
    18601 gcacggggca ggagctgccg cgccggtcca gttccagtaa tttctctggt ttattggcag
    18661 caacgtccat ctcatcctga gggaccccgg tgggccagag caggcgcacg acgtcggcct
    18721 ctgccgcttc tccgagtccg gcaatgattt ctgtcggccc ctggctgaga taaccactgc
    18781 ttccggcgac ctcgaacttc tgtacctgtc cattggaaaa gacctcgact ttggtaccaa
    18841 tcgctgattt gttgtcattc aggccagcca gaagaatgcg cagagagtga ttccggcttc
    18901 ctcccgtgtt tcgcagcact actggtggcg cgttccattg tgtcacaatc aggtcagcgg
    18961 ccccgtcccc atccatgtcc gcggcaatca gagcgcgcgg gttggaaagc ctgattttgt
    19021 ccgctccggt tgcggcactc acatcttcaa agccctgcgg gccgcgattg cgccagatgt
    19081 gcagcgtagg tccgctggtg gtctgcacaa tggccgccag atcaatccag ccgtcattgt
    19141 ctatgtccag ggccgtgatg ccccaggcag acagaatgcc ctcggctggc aaaggaacac
    19201 gttcaaactg cctgcctttt atgttcttcc acaggctgat gccaggcgct ccggagtgcg
    19261 tgaggacgac atccatccat ccgtctttat tgaaatcgaa gacatagatg ccgcgggatg
    19321 gagaaagtcc tggctcggca atcaacggaa cggcccggaa agcgccttct cgctgatttt
    19381 catagaggat tggagacggg ccgcttccag ttaccagaag gtccacagcg cgatcattgt
    19441 taatgtcaga aagcatggcg gccacagtgt ctgtcttccc ccccagaccg gttggcgccg
    19501 tccactcagt aaaagtcgag ttgccattgt tgcgccagag aacattcggc cccttcccgg
    19561 gcgaaatagc cgacccggtg acaaaaagat cgaggtcgcc atcatgatca aaatccacaa
    19621 acgtcaggcc agatgggtga ttcaactgtg caatggctac agtgctggtg acatctgcaa
    19681 attttccgtg tccgaggtta tggaagagga caatgcggtc actgagtgcc acggcaaggt
    19741 caggcaggct gtcattatcg tagtctccaa cagcacaggc cgtggcatgg ccggagagcg
    19801 caagccccgt ccgttcagcc ggtatctctt ctaaggaacc atcggcggat gcgcggtaga
    19861 tgtgcagagc gcggtccccg cttcccagag caattatgtc ttttttgcct tgtccttcca
    19921 catcgagcac acaggcgcca gcaccagcgg cgtccggcga ggcttcgctg gcagacgtgt
    19981 tagtcgcagc gtgtatgaac tgcggtacaa atcgcaccgg tatcatggga ccaacgggtt
    20041 tttccggcgc aagcatgtcg agtgcctttg cgccactgcc ttgttcgcca taggtgatgg
    20101 taaacggcgt acctattttg gtctgcatga tggtctggaa gcgcctgaga tgttcgcggg
    20161 cctccatcac ccggcccagg cgctgcaggg cactggccag cccgaactgg gcggaagcat
    20221 gaagcggatt gagcctcagg gctgcttcaa attgctggac cgcgttgttg tattctcgca
    20281 ggttcaggta cacagagccg agatagtagt ggctgtctgc atcttccggg tcgatctgaa
    20341 tggcgcgctc aaagtccctg gccgccagcg caaggttatt tccagccatg tgtgtaagac
    20401 caagactgta ccagatgtgg agattgccgg gatccatctt tgcggcccgg agaaaggccg
    20461 cctcggcttc cggcagctta cgcagataca gaagtgcgat tccctggttg atgaccggaa
    20521 tgggggagga cggttctatc ttgccagcct ctgtgaattt ggctagggcc ttttccagca
    20581 gttgctgatt aagaagggcg accccggtat tgttggctct tgcagcggtc aacgtgttag
    20641 catcacccgg accagccagc gctgtcacga ccagaatcgg gctgaagctc aggaaagcca
    20701 gtaaaaacaa aattctgcag gatatgaaaa gaaaagacgc tctgatgcgt atggacatga
    20761 gcaaggcagt gtggttggac gttaccgcgt ttgattataa ctttacgagg acatgaagtg
    20821 atgccgggtt acgcatgagc gtcctgaggc cagtgagaaa tctgttcccg agtgtgctgc
    20881 tcgcgcatct tgctatcttt gccttgaacc tataacgtgg atggaaatca tttccccggt
    20941 agaggtattt cagcaagaat gttgcccacg gccatcggct caaccagact ataaaggacc
    21001 actatcctga aataaaagga cattgcacaa ccatgaatac atttgttcgc cggagcggtt
    21061 tgtttttcct ttgtctggtt ccttctctct cgctggcgca gatgccgggc cagaagatac
    21121 cgggacaacc taccatcaca accgcccaga caagtagtgg cttgcgtgcc accataggca
    21181 aagagcagat ggagattaca gtttgcggcg attctgtaat tcacgtggtg gcaacgccgg
    21241 atccggaggc gaaaccctct cctaaaccgt ggatgctgga ccatgctcag tcctgccctg
    21301 gggcgacctt ccggtattcg cagcatgacg gccgggcaac gttggaaact tcgcgtctga
    21361 aagtcgagct ctctctggac cggggaaacg ttgtctttca gacggctgcc ggggagacgc
    21421 ttctgcggga aggcggcagt gttccccgca cctatgagcc tgccgaactg aatggcgagc
    21481 aaacgtttca catcaccgac cgcttttctc cgaatgtcac cgaggccttt tacggattag
    21541 gccagcacca gagcggaatg ttcaattatc gtggctcgac ggtcgagctg gggcagaaca
    21601 atacggacgt cgccatcccg ctgctggttt caagcaacgg ctacgccatt ctctggaaca
    21661 ccgcagcgct gacatatgtg gacaaccgct ttccatcgga gctggccttc cgctcactcg
    21721 cgggccatgc cgtggactac tacttcatct atgggccgga gatggacagc atcatccacc
    21781 agtaccgtag catgaccggg cacacgccca tgctgcccaa gtgggcctat ggcctgttcc
    21841 agtccaaaga ccgctatgtt tctctggacg agattctgaa gattgcgggc cgctatcgcg
    21901 cagagcacat tcctctagat gcgatggtgc aggactggtt ctggtggaag cacgaaggcg
    21961 atccggtctt caatgaaaac tatcacgatg ttcccggaga cctgaaaaag ctgcaccaga
    22021 tgaacgtcca cgccatgatc tccgtctggg gactcttcga tcctgcttcg gaaaatttca
    22081 aggagcttac ggaaaaccac ttcgatgtgc ccggcgcgca tgtgtacgac gcgaccaacc
    22141 ccgctgcgcg cgatttttac tggaacaagc tggcaggcaa gctctttgcg cagggatggg
    22201 acgctttctg gttggacagc gccgagcctg aagaatactg gccgcacatg ggcgatgcca
    22261 tcctccagaa caagcggctc tatatcggca acggcgcgga gttcacgaat atttttccct
    22321 tcatgcacac caccggagtc cagcagcact ggaaagagac gaccgacagg aagcgtgttt
    22381 ttctgctgac gcggtcggcc tttctgggcc agcagcgcgt aggcgctacg gtctggtcgg
    22441 gtgatgtcta caccacgtat tgggccctgt cgcaccaggt cccggcgggg ctgaactttg
    22501 ctctctccgg ttatccctac tggacaaccg acattggagg ctactggcca catgaaaatg
    22561 atgtgattca cgaccctgcc tatcaggagc tttatgcccg atggtttgaa ttcggaacct
    22621 tctgccctgt cttccgcacg cacgggcacc gcgcggaaaa cgagatgtgg gcgtatgaca
    22681 a
//
LOCUS       DASRBV010000026         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_660328, whole genome shotgun sequence.
ACCESSION   DASRBV010000026 DASRBV010000000
VERSION     DASRBV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660328"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFA02_01165"
     CDS             <1..1128
                     /locus_tag="VFA02_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08484.1"
                     /translation="TYPLNRDPQVREAFDHFYIMDYNGAISRFEAVQGQHPTDPIATD
                     YLLYVTLFKELFRLDLLDSTFYANDGFLTGKHTVVQDPQVSDQIKSLTDKAVDQANTQ
                     LKSNANDVNALFARGWARSLEATYMAMVERSFGAALHLALAARTDHSRVLQLDPNYVD
                     AKLVVGVYEYVIGALPLPFKILIGIAGIHGSKATGMEMLRDAASRGIISSVESRTCMM
                     LFLRREAKYQEATQIAQSLVAQYPRDFLFRLEVANLQKDAGLGPVAIASYREVVDLSK
                     RPGYFPATHLELVYFGLGDSLRGQKMYVDAVQAYRDGAFQPTTSPELKRRCLLAAGEV
                     YDLMHDREKAKQQYQAVMELGADTVQGQQAQKYMRSAYSGR"
     gene            complement(1187..>2374)
                     /gene="pckA"
                     /locus_tag="VFA02_01170"
     CDS             complement(1187..>2374)
                     /gene="pckA"
                     /locus_tag="VFA02_01170"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZU08485.1"
                     /translation="KQLFVRPQPSELENHMAEFTVIAAPEFEAVPERDGTKSSTFILA
                     DFTRKIVLIGGTKYAGEMKKSIFSVMNFLLPLRDVFPMHCSANIGADGETALFFGLSG
                     TGKTTLSADPTRRLIGDDEHGWSPTGVFNFEGGCYAKCVDLSEEKEPQIYRAIRFGAV
                     LENVVIDPATRMPDYSDIRYTENTRAAYPIEFIENAVIPGMGGHPKNVLFLTADAFGV
                     LPPIAKLTPDQAMYHFLSGYTAKLAGTEAGLGSEPVPEFSTCFGAPFLPLAPRVYAEM
                     LGRRLKEHNAQCWLVNTGWTGGAFGTGKRMSLKHTRSIVHAVLDGSLAGAEYVTEDAF
                     GLSIPVSCKDVPGEILNPRNVWADKHAYEKQARMLATKFEENFAKFDAPENVRAAGPK
                     ARR"
BASE COUNT          545 a          703 c          621 g          505 t
ORIGIN      
        1 acttacccgc tgaaccgcga ccctcaggtc cgggaggcct tcgaccactt ctacatcatg
       61 gactacaacg gcgcgatctc gcgctttgaa gcggtccagg gccaacatcc cactgaccca
      121 atcgcgacag attacctcct ctatgtcact ttgttcaaag agcttttccg gctcgacctt
      181 ttggactcga ccttttacgc caatgacggc ttcctgacgg gaaaacatac ggtcgtccag
      241 gacccgcagg tgagtgacca gatcaaatcg cttacagaca aagccgtaga ccaggccaac
      301 acacagctca agtccaacgc taacgatgtg aacgcgctct ttgcgcgcgg atgggccagg
      361 agccttgaag ctacatacat ggccatggtg gagcgcagct tcggcgcggc cctgcatctg
      421 gcgctcgcag cacgcactga ccatagccgt gtcctgcaac tcgaccccaa ctatgtggac
      481 gcaaaactgg tagtcggcgt gtatgagtat gtcatcggcg cattgcccct tccattcaag
      541 attctcatcg gcattgcagg tattcacggc tcgaaggcaa ccgggatgga gatgctgcgc
      601 gatgccgcct cccgcggcat cataagctcg gtggagtcgc gtacctgcat gatgctcttc
      661 ctcaggcgcg aagcaaagta tcaggaagca acgcagatcg cgcagtcatt ggtggcgcaa
      721 tatccgcgcg atttcctctt tcgccttgaa gtcgccaacc tgcagaagga tgccggactg
      781 ggaccggttg caatcgccag ctaccgcgag gtggtggatc tgtcaaaaag gcccggctac
      841 ttccctgcta cccatctgga gcttgtgtac tttggtctgg gcgacagcct gcgcggacaa
      901 aaaatgtacg tcgacgccgt gcaagcctat cgcgacggcg cattccagcc cacaacaagc
      961 cctgagctga aacggcgctg cctccttgcc gctggagagg tctatgacct catgcacgac
     1021 cgcgaaaaag caaaacagca gtatcaggcc gtgatggaac tgggcgccga cactgtccag
     1081 gggcaacagg cccagaaata catgaggtct gcatattcag gacgttaatg tccgggcaaa
     1141 gcctgactct gccctcctgg ccggtccaat aacagccgcg atatggctac ctgcgtgctt
     1201 taggtccggc agcgcggaca ttctccggtg catcgaattt tgcaaagttc tcttcaaatt
     1261 tcgtggccag catgcgcgcc tgtttctcat aagcgtgctt gtccgcccaa acgttgcgtg
     1321 ggttcagaat ttcgcctgga acatctttgc atgaaaccgg aatggaaagc ccgaaagcat
     1381 cttctgttac atactcggcg ccggcaagac tgccatccag aacagcatgg acgatggagc
     1441 gcgtatgttt caggctcata cgctttcctg ttccgaatgc gccgcccgtc cacccggtat
     1501 tcacaagcca acattgagcg ttgtgctcct taagccggcg tcccagcatt tctgcgtaca
     1561 cacgcggcgc aagcggcaga aacggcgccc caaaacaggt ggaaaactcc ggaacaggct
     1621 cgctgccaag tcctgcttcc gtgcctgcga gttttgccgt atatcccgac agaaagtggt
     1681 acatggcctg gtcgggtgtg agcttcgcaa tgggcggcag aacaccaaat gcgtccgcag
     1741 tcagaaagag cacatttttc ggatggccgc ccataccagg aatcacggca ttttcaataa
     1801 actcgatcgg ataagcagcg cgcgtgtttt ctgtgtaccg gatatcagag taatccggca
     1861 tgcgcgttgc cggatcaatc acaacgttct caagcacggc gccaaagcgg atagcgcgat
     1921 aaatctgtgg ttctttttct tctgacaaat ctacgcactt ggcgtaacat ccgccctcaa
     1981 aattaaacac gcccgtagga ctccagccat gctcgtcatc gccaatcaga cggcgcgtcg
     2041 ggtctgccga gagtgtggtc tttccagttc cggaaagtcc gaagaaaagc gccgtctcac
     2101 catctgctcc aatgttcgcc gaacaatgca tggggaaaac atctcgcagc ggcaacagaa
     2161 aattcattac gctgaagatg gacttcttca tttcgcccgc atacttcgtg cctccaatca
     2221 gcacgatctt gcgcgtgaag tcagccagaa tgaaggtgct cgactttgtc ccgtcccgtt
     2281 cgggcaccgc ttcaaactcc ggagcagcga tgaccgtgaa ctcggccata tggttttcaa
     2341 gttcggaagg ctgcggtctt acaaaaagct gttt
//
LOCUS       DASRBV010000027         6599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_609359, whole genome shotgun sequence.
ACCESSION   DASRBV010000027 DASRBV010000000
VERSION     DASRBV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609359"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFA02_01175"
     CDS             complement(<1..797)
                     /locus_tag="VFA02_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU08486.1"
                     /translation="MRLCNNRKVFFFFLLIAWLPLSAQQQQQQPPLNVDRDPVVSPDA
                     ADNQPVSPSNPGRNAASSSETLEHGQHGGFTFRRDVDEVVLNATVLDDNGRIVNDLTK
                     DDFHVFEDGVPQTISSFQHQDIPVSMGILIDNSGSMRDKRAAVNTAALDLVRASNPQD
                     EAFVVNFSDEAYIDQDFTSAINKLQDGLAHIDSKGGTALYDAIVASADHLAQGAKRPK
                     QVLLVVTDGEDNASSLNLEQTIRRVQDLQGPVVYSIGLLFGDSGGGR"
     gene            complement(781..1860)
                     /locus_tag="VFA02_01180"
     CDS             complement(781..1860)
                     /locus_tag="VFA02_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU08487.1"
                     /translation="MALSSGRNTAISTAAPAGELSIPQLLAVLLVLVFTAGVLLPHAR
                     AQEDSLDTVHVQPPPPKPDPNAPPPPAEGDAAMSASRGERIRVDVNLVLVPLTVTDPM
                     DRLVTGLEKENFFLYENNKLQAIKTFSQEDAPVSIGIIFDMSGSMANKIIRARDSILQ
                     FMHTANPQDEFFVIGFNDRPELIEDFTSNPQDIEARLTNVRGQHRTALLDAIYFGLNK
                     MKQAKYERRALLIVSDGGDNRSRYTENEVRATVREANVQIYAIGIFDPYAATTEERLG
                     PVLLNDICSETGGRLFRVDDVSEMGDIAEKISAELRNEYVLGYKPDDPRKDGKWRKLK
                     VKLVPPPGLPQLTVHARTGYYAPLQ"
     gene            2171..3505
                     /locus_tag="VFA02_01185"
     CDS             2171..3505
                     /locus_tag="VFA02_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZU08488.1"
                     /translation="MARIYPFYALRYNPSLVRVQDCVTQPYDKITPAMQQAYYQKSPY
                     NLVRIILGLPELFDGKETGDVYTRAARDFKSWREQGILAQEPEPCIYAYSQKFTVPGS
                     NGKMLERRGFIALGGLHDYSEGVVFRHEQTLSKPKSDRLNLLRATRAHFGQIFMLYSD
                     PGLTAEKLLFSEDAIPEIEVTDEYDVLHRVWKISDPNTINILISALADKKLIIADGHH
                     RYETALNYSKEHAPAQPVSSERSTNSLPQPPYPEAAVMMTFVNMDAEGLVILPTHRVV
                     FGLKDFSPAAFLDKARQFFDIEPVASREAGLLTAKLAAEGTGKTAFVAVTKDGNSLLK
                     ARPDAIVNALSGLSERQRQLDVAQLHGIVLEKLLGLTPEAVREQTNLRYLRDAGEAVE
                     QVLRGDADVAFLMNPVTMAQLREVAFAGEVMPQKSTDFFPKLLSGLAIYPLD"
     gene            3531..4166
                     /locus_tag="VFA02_01190"
     CDS             3531..4166
                     /locus_tag="VFA02_01190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZU08489.1"
                     /translation="MAQQSAVPSVPLPPRAQVRPQYIVVLDAAHGGTDSGARLSDNLF
                     EKDVALALALRLRAALDAQGIQVVTTRDSDAAVPLQARAEAANHALAEACITLHATGT
                     GTGVHLFTSSLAPAPRVQFLPWRTAQAAFVTQSLRLSSEINAALAHVAVPVILGRTSL
                     QPMDSFACPAVAVEVANAKNVPLTDAAYQKKIVDALTSALTQWKNDWRPQP"
     gene            4163..4747
                     /locus_tag="VFA02_01195"
     CDS             4163..4747
                     /locus_tag="VFA02_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZU08490.1"
                     /translation="MIPRIQRILFWAMLLSAVIMAVVLIRLRERAHDRLMTAADNMPA
                     SAPLNAPVEKIELLVANDADGSLIPVERSFPMPKDPNARARVLLQKLVEEYSAPKSTH
                     PIPNASGVDEVFLMPLPQQKGEMAVVDLDSAFVEAHPSGIEPETLTLLSMIATLHANL
                     PQITEVRFLVNGQQKDTLAGHADLTRTYETAVRQ"
     gene            4744..5538
                     /gene="murI"
                     /locus_tag="VFA02_01200"
     CDS             4744..5538
                     /gene="murI"
                     /locus_tag="VFA02_01200"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZU08491.1"
                     /translation="MRIGVFDSGVGGLTVLRALLRQLPAADYIYLGDTARLPYGSKSQ
                     ATIARYAVSSAHFLVEEQGAEFLVIACNTASALALDAIKAAVSVPVLGVVETGANAAK
                     LASQTGDVLVIATAATVQSHAYSAACRERGLRAYEKACPLLVPLVEEGWIDHPVTQEV
                     LRIYLTELREEASQAQMSPDTLVLGCTHYPLLRTQIERMVPELQVIDSACVTAEQVTA
                     AINGKKTTGNGARRFFATDSIEKFQNMGTRFLNEPIEDVALVDLGG"
     gene            5642..6409
                     /locus_tag="VFA02_01205"
     CDS             5642..6409
                     /locus_tag="VFA02_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZU08492.1"
                     /translation="MLNLKDRVAVVFGVANKRSIAWAIVQKLHEAGAKLAIGYQNERL
                     KAEADSLIAELPGAEAFQCDVSSDAEIAQVFEQLKTRYGKIHTLVHSIAYAPADELKN
                     DFILTTREGFRIAHDVSVYSLIALSRAAAPLMTEGGSIMTLTYYGAEKVVPHYNVMGV
                     AKAALEATVRYLAADLGKQNIRVNAISAGPIKTLAARGIGGLSDMLKAHAERSPLGRN
                     VEQAEVGGTALYLASDLASGVTGETIYVDCGYNIMGF"
BASE COUNT         1378 a         2071 c         1818 g         1332 t
ORIGIN      
        1 tcgcgtcccc cgcctgaatc cccaaacagt agccctatcg aatagacgac cgggccttgc
       61 aggtcctgca cgcggcggat cgtctgttcc agattcaggc tcgacgcatt gtcctctcca
      121 tcggtgacca ccagcagcac ctgcttgggg cgttttgctc cctgcgcaag atggtcggcc
      181 gaggccacaa tcgcgtcata cagcgccgtg ccgcccttcg aatcaatgtg cgccaggccg
      241 tcctgcagct tgttgatggc cgaggtaaag tcctggtcaa tgtaagcttc atcagagaaa
      301 ttgaccacga acgcttcatc ctgcgggttc gatgccctca ccagatcaag cgcagccgtg
      361 ttgaccgcgg cccgcttgtc gcgcatcgag ccggagttgt caatcagaat gcccatcgaa
      421 accggaatgt cctggtgctg gaaggaagaa atcgtctgcg gcacgccgtc ttcaaaaacg
      481 tgaaagtcgt ctttcgtcag gtcgttcaca atgcggccgt tgtcatccag cactgtcgca
      541 ttcagcacaa cttcgtctac gtcgcgccgg aacgtaaagc cgccatgttg gccatgctca
      601 agtgtttctg aagaagatgc agcgtttctg ccgggattgg atgggctgac gggctggttg
      661 tcggccgcat caggtgaaac cacagggtcg cggtccacat tcagcggagg ctgttgttgc
      721 tgctgttgcg cgctaagcgg cagccacgcg atcagaagaa agaaaaagaa gactttccgg
      781 ttattgcaaa ggcgcatagt atccggtgcg agcatgcaca gtcagctgag gcaggcccgg
      841 gggcggcacc agctttactt tcaatttacg ccacttgcca tcttttcggg gatcatcggg
      901 cttatatccg agcacatatt cgttgcgcag ctcggcgctg attttctcag cgatgtcgcc
      961 catttctgaa acatcgtcca cgcggaacag acgccctccc gtctcagagc agatatcgtt
     1021 cagcagcacc ggccccaacc gctcttccgt agttgcggca taggggtcga agatgccgat
     1081 agcgtaaatc tgcacattgg cctctcgcac cgtcgcccgg acttcgtttt ccgtatagcg
     1141 gctgcggttg tctcctccat cagagacaat cagcagcgcg cgccgctcat acttggcctg
     1201 cttcatcttg ttcagaccaa aataaatcgc gtccagcagc gccgtgcggt gctgtccacg
     1261 cacgttggtc agccgcgcct caatgtcctg cgggttcgac gtgaaatctt caatcagctc
     1321 cgggcggtcg ttgaagccaa tcacgaaaaa ttcgtcctgc ggattggccg tatgcatgaa
     1381 ctggagaata gaatcgcgcg cccgaatgat cttgttcgcc atgctgccgc tcatatcaaa
     1441 aatgattccg atcgagaccg gagcatcttc ctgcgaaaaa gttttaatgg cctggagttt
     1501 gttgttctca tagaggaaga agttctcttt ctcaagcccg gtgaccaggc ggtccattgg
     1561 gtcggtcacg gtaagcggca ccagcacaag gttcacgtcc acgcgaatgc gctcaccgcg
     1621 cgaggcgctc atggcggcat cgccctccgc tggcggtgga ggagcattcg gatcaggttt
     1681 cggtggaggc ggctgaacat gcaccgtgtc gagcgaatct tcctgcgcac gggcatgtgg
     1741 aaggagaact cccgcggtga aaacaaggac aagcagcacc gcgagcaact ggggtatgga
     1801 aagctcgcct gccggagcgg cagtggaaat cgcggtattg cgccccgacg agagcgccat
     1861 gacgtgcttt acccccgggt cccttctgtg cagaatgcta gcacgcccag aaaaaggatg
     1921 cttctgttct tttcgatgtc atgggaagaa aaaacgtcca gcccttgccg aaaaggactt
     1981 ttacatgacc acagcatttc cggtgccttg ctgacggcct gcaagggcag gcgctggctg
     2041 cttttcagct tttccatctc ccgccttctt acaacgaaaa atcccgcccc ttcgggatac
     2101 aatgcagtca gaaccactct tttccagtac aacctgattc tgattttccg ggagatttac
     2161 aaccgctcgc atggcccgta tttatccgtt ttacgcactt cgctataacc cttcccttgt
     2221 tcgcgtacag gactgcgtga cccagccgta tgacaaaatc acgcccgcga tgcagcaggc
     2281 ctactaccag aaaagcccct acaacctggt gcgcatcatc ctgggcctgc ccgaactttt
     2341 cgacggcaag gagaccggcg acgtctatac gcgggccgcg cgcgacttca aatcgtggcg
     2401 cgagcagggc attctggcac aggaaccgga gccttgcatc tatgcctatt cgcagaaatt
     2461 caccgtgccc ggctcgaatg gtaagatgct cgagcggcgc ggcttcatcg ccctcggcgg
     2521 actgcatgat tacagcgaag gcgtcgtctt ccggcacgag cagacactct caaagcccaa
     2581 gtccgaccgc ctgaacctgc tgcgggccac ccgtgcgcac ttcggtcaga ttttcatgct
     2641 gtattctgac ccgggcctca ccgccgaaaa actgctcttc tcagaagacg ccattcccga
     2701 aatcgaagtt accgacgaat acgacgtcct gcaccgcgtc tggaagatca gcgaccccaa
     2761 caccatcaac atcctcatca gcgcgctggc ggacaagaag ctcatcatcg ccgacggcca
     2821 ccatcgctat gaaaccgcgc tgaattattc aaaagaacac gcccccgcgc agcctgtttc
     2881 aagcgagcgc agcaccaatt cgctgcccca gcctccttat cccgaggccg cggtcatgat
     2941 gacctttgtc aacatggacg ctgaagggct ggtcattttg ccgacgcacc gcgtcgtctt
     3001 cggcttgaag gacttttctc cggcggcatt tctagacaaa gccagacagt tctttgatat
     3061 cgaaccggtt gcctccagag aggccggtct gctgacggca aaactcgctg ctgaaggcac
     3121 aggcaaaaca gcctttgtcg ccgtcacaaa ggacggcaat tctctgctga aggccagacc
     3181 cgatgccatc gtcaacgcat tgtccggcct ttccgaacgc cagcgccaac tcgatgtcgc
     3241 gcaactgcac ggcattgttc tcgaaaaact gctgggcctc acgccggaag ccgtgcgcga
     3301 gcagaccaac cttcgctatc tgcgcgatgc cggcgaggcg gtcgagcagg tgctgcgcgg
     3361 tgatgccgat gttgccttcc tgatgaatcc ggtaacgatg gcgcagttgc gcgaagtggc
     3421 cttcgccgga gaagtcatgc cgcagaaatc cacggacttt ttccctaaac tgttgagtgg
     3481 ccttgcgatc tatccactgg attagttgcg tcctgctctg cgcgcctgtt atggcgcaac
     3541 agtcggcggt cccttccgtt cctctgccgc cccgggcgca ggtgcggccg caatatattg
     3601 ttgttctgga tgcggcgcat ggaggcacgg actccggcgc gcgcctctca gacaatctgt
     3661 tcgaaaaaga cgtggccctg gccctggccc tgcgcctccg cgcagcactc gacgcccaag
     3721 gcattcaggt cgtgaccacc cgtgattccg acgcggccgt gccgcttcag gcccgcgcag
     3781 aggctgcgaa ccacgcgctg gccgaggcct gcatcaccct ccacgccacc gggaccggaa
     3841 ccggcgtgca cctgtttact tcttcgctgg cccctgctcc gcgtgtgcag ttccttccct
     3901 ggcgcacggc gcaggcggcg ttcgtcacgc aaagcctgcg gctttcctct gagatcaatg
     3961 cagccctggc ccatgttgct gtcccggtca ttcttggcag gacctctctg cagcccatgg
     4021 acagctttgc ctgccccgct gtagcagtcg aagtggccaa tgcaaagaat gtccctctga
     4081 ccgacgcggc atatcagaaa aaaattgtgg acgcgctcac ctccgccctg acgcaatgga
     4141 aaaacgactg gaggccgcag ccgtgattcc ccgcatccag cgcattctct tctgggcaat
     4201 gctgctttcc gccgtcatca tggccgtggt gctcatccgt ctgcgcgagc gcgcgcacga
     4261 ccgcctgatg accgctgcgg acaacatgcc tgccagcgcc cccctgaatg ctccggtcga
     4321 aaagatcgaa ctgcttgtgg ccaacgatgc cgacggttcg ctcatccccg tcgagcgcag
     4381 cttccctatg ccgaaggacc ccaacgcccg cgcacgcgtc ctgctgcaaa aactggtgga
     4441 agaatactcc gcgccaaagt ccacccaccc catcccgaat gcctccggcg tggacgaggt
     4501 cttcctgatg ccgctcccgc agcaaaaagg ggaaatggcc gtggtagacc tcgacagcgc
     4561 gtttgttgag gcccatcctt ccggcatcga gcctgagaca ctcaccctgc tctccatgat
     4621 tgccacactg cacgcaaacc ttccgcagat tacagaggtg cgctttctcg tcaacggtca
     4681 gcagaaggac acactcgccg gacatgccga cctgacacgc acctatgaga cggcggtgcg
     4741 ccagtgagaa tcggcgtctt tgattcaggc gtgggcggcc tgacggtgct tcgcgcctta
     4801 ctgcgacagc ttcccgcagc ggactacatc tatctgggcg ataccgcgcg tctcccctac
     4861 ggctcaaaat cgcaggccac gattgcgcgc tacgccgtat ccagcgcgca ttttcttgtt
     4921 gaagagcagg gtgccgagtt tcttgtcatc gcctgcaata cggccagcgc cctggccctc
     4981 gatgccataa aagcagccgt ctcggttccc gtgctcggcg tcgtcgagac gggcgccaat
     5041 gccgcaaaac tggcctcgca gacaggcgat gtgctggtca tcgccaccgc tgctaccgta
     5101 cagagccacg catattctgc cgcctgccgg gagcgcggcc tgcgcgcgta tgaaaaggcg
     5161 tgcccgctgc ttgtccctct cgtcgaagaa ggctggattg accatccggt cacgcaggaa
     5221 gtcctgcgca tctacctgac tgagttaagg gaagaggcca gtcaggcgca gatgtcgccc
     5281 gacacgctgg tgctgggatg tacccactac ccgctgcttc ggacacagat agaacgcatg
     5341 gtgccggagc ttcaagtgat tgactcggcc tgcgtgacgg ctgaacaggt cacagcagcc
     5401 atcaacggaa aaaagacaac aggaaatggc gcgcgccgat tctttgccac tgactccatt
     5461 gaaaaatttc aaaacatggg aacgcgcttc ctcaacgagc cgatagagga cgtggccctg
     5521 gtagacctcg gcggctgata tccttacaag tgcttttgca tacttctcat ccttaggacc
     5581 cagagccgcg cgaaggagag aagggaactg ctttttgctt ttccggaggt aagaaaattt
     5641 catgctgaac ctgaaagacc gtgtcgccgt cgtctttgga gtcgccaaca aacgcagcat
     5701 cgcctgggcc atcgtccaga agctgcacga agccggcgcg aagctcgcca tcggctacca
     5761 gaatgagcgc ctgaaggccg aagccgacag ccttatcgcc gaacttcccg gcgcggaggc
     5821 cttccagtgc gatgtttcga gcgatgcaga aatcgcacag gtctttgaac agttgaagac
     5881 gcgctatgga aagatccaca ccctggtgca cagcatcgcc tacgccccgg cagacgagct
     5941 gaagaacgac ttcatcctga ccacacgcga gggcttccgc attgcgcacg acgtaagcgt
     6001 gtattcgctg attgcattaa gccgtgccgc tgctccgctg atgaccgagg gcggaagcat
     6061 catgaccctg acctactacg gcgcagagaa ggtcgttccc cactacaacg tgatgggcgt
     6121 ggccaaggcc gcgctcgaag ccacggtccg ctatctggct gctgacctgg gcaagcagaa
     6181 cattcgtgtg aatgccatct cggccgggcc catcaagacg ctggccgcgc gtggcatcgg
     6241 cggactcagc gacatgctga aggcccacgc cgagcgctcc ccactggggc gcaacgtgga
     6301 gcaggcagaa gtaggcggca ccgcgctcta tctggcctcc gacctcgcca gcggcgtcac
     6361 cggagagacc atctacgtag actgcggcta caacatcatg ggcttttagg gccgggcact
     6421 gaagaaacgt aagattcgct gatctgcatc cttccagaaa cagctctata acaccaattc
     6481 ccggatgaca atctttcagt gcctgcatct ggacaggcac ttcttttttc gtgacaccaa
     6541 agcaagtgca gtagcactat gtgtggtgcg ggcccccgct gcataagaag tcacgatga
//
LOCUS       DASRBV010000028        14313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_558363, whole genome shotgun sequence.
ACCESSION   DASRBV010000028 DASRBV010000000
VERSION     DASRBV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14313
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558363"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="VFA02_01210"
     CDS             complement(<1..1730)
                     /locus_tag="VFA02_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08493.1"
                     /translation="MIRRCRHHWVSLAALTLAGISSHAQQPAVKRDPAAATRAFLEGA
                     KALKKDDLRAAVKDFQRAAQLDPENRDYSTSFIIAKEHLLTKLVQEAGKARLLGNDAA
                     ARADLAEAYTLDPQNPIVAQHLDELARDNGPHDAVIMPAVEQAAPPVQLAPNPGKLSF
                     HLRSSATEVLRQVLHAYGIIPTLDASVPTPLVRFDVDDVDFAQAAQMLKLATGTFFVP
                     LDEKRVLVAADTKENRTKFERQVMETVYLPGLTTDEATEIGNAVRQVFEVQQATVQQE
                     QHTLTVRGPQERIEALNHTLAELLSGRGQVLLNLRLYEIDQSRMVNLGAQLPQQTTVF
                     NVPSEVNQIIQQNQSAVQQIIASGLANPGDNLAIVAILIASGAVSGTVFNQPFALFGG
                     GITQSGITLDQSATLNLSLNSSDTREIDNVQLRVQDQAEATFRSGTRYPIVTSTYSNL
                     AGTSLGIPGISQAGISSYLQNLGINSASLESSLNTTIPQVQYQDLGLTLTTTPRIQQD
                     HDVSLKLDLKITSLTGQTLDGNPILSNRQYTGVVTLAQGESALLVSNLTKQQSAAVSG
                     VPGLSELPGFL"
     gene            complement(1727..2461)
                     /locus_tag="VFA02_01215"
     CDS             complement(1727..2461)
                     /locus_tag="VFA02_01215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU08494.1"
                     /translation="MSTAPASAPANPRICIQDGFRWDRQNAYLFDIDGTLLRSRDRIH
                     YDSFFESVRSVMGRELVLDGVTLSGNTDPGILHDAFRLAELDPIHWEPHLDAVFRHMC
                     SLVAARREQMDLVKMPGVDEILAWLHNQGAILGVGTGNLEAIGWVKLEVIGVRQWFTF
                     GGFSDRFAKRADMIAHAASLARSIAGPEATVCVVGDTPFDIAAAKANHLPTIAVATGH
                     YSFDDLLVHQPEVCVTTLADLLASAR"
     gene            complement(2553..2849)
                     /locus_tag="VFA02_01220"
     CDS             complement(2553..2849)
                     /locus_tag="VFA02_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU08495.1"
                     /translation="MKRELDNLVAAMHAGGITYAEAVREFKRRYLTTVLAHHKGNQCK
                     AAKELGMHRNTLSRTLAELEIDTAQIRLGLKRPPRSERPVFEVKQGTTRFGVTS"
     gene            complement(2886..3422)
                     /locus_tag="VFA02_01225"
     CDS             complement(2886..3422)
                     /locus_tag="VFA02_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZU08496.1"
                     /translation="MALIDWAIVIILIVSVLSAAKHGFFVEAFSLAGVVLGVLLASWN
                     YQKLLPWIETWVHSSGAAEAIAFIAIAVAVMVAAGLTGRFIRWSVRTIGLGWADRLIG
                     AAFGLLKGCVLVTLGIMAVAAFLPHAAWIEKSRLAPYFLSAAHEAAIVTPAELSQRIR
                     DGVKLIRDAQPDWLKPQA"
     gene            complement(3522..4415)
                     /locus_tag="VFA02_01230"
     CDS             complement(3522..4415)
                     /locus_tag="VFA02_01230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZU08497.1"
                     /translation="MPALLETEFSDLVLHARGKVRDLYSVGEFLLLVATDRISAFDHV
                     LATGIPGKGKVLTQISLFWFDFLRDIVPNHLVTANVDEYPRELAGCKDELRGRSMLVK
                     RAQMFPVECVVRGYLSGSGWKDYQATGTVCGIRLPQGLRESDKLPEPVFTPATKSLHG
                     KHDENISFDEMVALVGARSAEKLRDLSLAIYRKASEHAESRGLILADTKFEFGTTAEG
                     IILADEVLTPDSSRFWPREGYKPGGAQPSFDKQYVRDYLESIRWNKQAPAPGLPDDVV
                     KKTQEKYFQAYELLTGKTLTL"
     gene            4622..4999
                     /gene="queD"
                     /locus_tag="VFA02_01235"
     CDS             4622..4999
                     /gene="queD"
                     /locus_tag="VFA02_01235"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HZU08498.1"
                     /translation="MYEVTVERGFSSGHYLRNYKGKCENPHGHNYKVRITLRGEKLDA
                     AGLLLDFKDLKHVMRPVIERLDHQMLNDLEPFTEINPSAENLARYFFDETNRQLAEMT
                     GGRVRVKDCTIYETDTTTATYYE"
     gene            5006..5686
                     /locus_tag="VFA02_01240"
     CDS             5006..5686
                     /locus_tag="VFA02_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU08499.1"
                     /translation="MYLIEIYKSVQGESSFAGRPCIFVRLAGCNLRCSWCDSEYTFKG
                     GYKLSEEEVVAEIEKLAPVKLIEFTGGEPLLQERELVPLMERLLANGYELMIETSGER
                     PLERVPAGVHKIVDVKCPASGEGGSFRMSNLEFLTQGDEVKFVIADRGDYEFARDFIR
                     QHALEVRVGHVLLSPAFNKAASTERSTANCLLDPRELVEWMLADGLDARMSLQIHKFI
                     WEPQKKGV"
     gene            complement(5720..6364)
                     /locus_tag="VFA02_01245"
     CDS             complement(5720..6364)
                     /locus_tag="VFA02_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08500.1"
                     /translation="MMRLRSLLSIVLLALCVPASPAFEHGPPPAKDATTYPAVDVHPD
                     EHVAIAAEPYNTAEKIKFFRVNYLDHQFLPIRIIITNSGDRPISLDQARIYFISAAGD
                     RIPAAEPDDIERRISLRDKKGADIPVGPIRLHTKPKTPNSKVEQDFSEFEYQALVVEP
                     HTTRAGFLFYDIQGLGSSPLHGAKLLLRQLKDTDGKELFYFEIPFDKYLQTQPQ"
     gene            6422..7678
                     /locus_tag="VFA02_01250"
     CDS             6422..7678
                     /locus_tag="VFA02_01250"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HZU08501.1"
                     /translation="MIFYSLGLALTLVLGAPYWLFRMAASGKYREGLGERFGRVPERL
                     LHGDGRPVIWVHAVSVGELLAASRMVLELRERMPEWRVVVSTTTRTGQALARERFGNE
                     NVFYFPLDFAFAVRRYLRALNPRMLVLMETEFWPRLLMECRRARIPVAVVNARISDRS
                     WPRYFRLRFFWKQMLNGLALVLAQSELDVERLRAIGAANVRYGGNLKYDIRTVVPAAV
                     TQKLKELIAAGTKVLVCGSTLEGEEKALLEAAPSDALVVLAPRHPERFEAVAQLLKQS
                     GRPWVRRSQWMEQPKPLRGGDIFLLDSMGELASVYSLATVAWIGGSLVPAGGHNPLEA
                     AQFRVPVVMGPHYENFRAIVDRLRAADAIRIANPNEVGSVLASLLSDRELAHNIGERA
                     YRIFTAESGATVRAAESLLELAGGKA"
     gene            7678..8643
                     /gene="lpxK"
                     /locus_tag="VFA02_01255"
     CDS             7678..8643
                     /gene="lpxK"
                     /locus_tag="VFA02_01255"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZU08502.1"
                     /translation="MRRLLWPLNPVYAASVAAKNVAYDRGWLKTKRTQWPVVSVGNLS
                     AGGSGKTPFVIALARLLVRNGVQADVLSRGYGRSADAIEKVNPSGDAARSGDEPLLIA
                     QSAGVPVYVGADRYEAGLVAEKNESGPRIHLLDDGFQHRRLARDLDIVLLHRTDFSES
                     LLPVGNLREPLSALRRASAVVLRQEDAAFEENLRERGIEAPVWLIRRVMNVPFDAGRV
                     VAFCGIARPGEFFSLLRANGAEVADTLAFRDHHRYTEDDLKRLIGLAKSAKADAFLTT
                     EKDSVRLPQEMKERLSAVAPLRLVPLETRFEDMSAVLKKVLPLAG"
     gene            8722..9780
                     /locus_tag="VFA02_01260"
     CDS             8722..9780
                     /locus_tag="VFA02_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZU08503.1"
                     /translation="MPIKTQQLRVLIVRLGAMGDILHALPAVTALRAAHPEWFLGWAV
                     EPQWRGLLAAHGATARGPEMPVVDQIHVVPAKQWGRNPLHVSTVEDIRRLRREFRAAR
                     YDVCVDLQGAVRSAVVGKWAHPQRLIGEAIPREWAAQYLFDERVETHGVHVIEQAIEV
                     ANEVAGESLPVMQPWLPLSESAERRAAEFHGPLVILNPGAGWGAKRWPAERYGAVARV
                     LAGKGFQVVINVGPGEEQLAQEVSGASGGVSLPVSPHLDELIALTRRASLVIAGDTGP
                     LHLASALGIPVVGIYGPTDPARNGPFGGKFRVLRHPESKRDHSRRTEPEAGLLTISAE
                     DVLAAAIDLLEENASNGR"
     gene            9770..10324
                     /locus_tag="VFA02_01265"
     CDS             9770..10324
                     /locus_tag="VFA02_01265"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZU08504.1"
                     /translation="MAADWSRIARRIRVPLGFVFAVVYILLARPTFVSMMWSLLLVLP
                     GLWLRAYASGYVKKNAELTVTGPYAYTRNPLYLGSMMIAFGFAEASRSIVTVLLLAVL
                     FAVIYLPVIGSEERFLRSTFKGFDEYAAKVPRLLPRLRPARLLQNGAGEFSFDLYRKH
                     REYNALMGAAAIYAVLALRIIYFH"
     gene            10382..11119
                     /locus_tag="VFA02_01270"
     CDS             10382..11119
                     /locus_tag="VFA02_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZU08505.1"
                     /translation="MLLPGGKAQSPQGLPSLPAPHPGLGLPARQTLNYSVDWRVFPAG
                     TVSFHQEAEGDAQHITAVADSIGAVNLIYRVSDRFQSSFSRRTGCSTAFSKQLQEGRR
                     QVNSTLNFNYGQGKAILEEKNLVSGIAKHQEAPVPSCVSDLLSAIFYTASQPLQVGQS
                     FQIPVYDAMRTIPVTMKVESRESVRTPSGTYQTVRVQPTADAGVVKNRGNIWIWYTDD
                     ERHIPVQMRARLFWGTLTFQLTGIEQK"
     gene            complement(11123..11461)
                     /locus_tag="VFA02_01275"
     CDS             complement(11123..11461)
                     /locus_tag="VFA02_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08506.1"
                     /translation="MSKKHAPYGGCTMGLSEAGNSPEFQQSHFDSSQQTPEEIQEEQR
                     KIRRLQIMMNMVMSVISQDNTLTIDEASEMVADTRRAALAMFPGKELAWDIIYRPRLQ
                     RLMRERYRIQ"
     gene            complement(11545..13029)
                     /locus_tag="VFA02_01280"
     CDS             complement(11545..13029)
                     /locus_tag="VFA02_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZU08507.1"
                     /translation="MTVTRRNFLVTSALTFGSLYASGEAVPPPAPFGALPSARQLRWQ
                     RMETNAFLHFTVNTFTDKEWGDGDEDPSIFNPQGFDPDAIVSALKAGGMKGVILTCKH
                     HDGFCLWPTKTTDHCIRNSPWKNGKGDIVRDISQAAQRHGLKFGIYCSPWDRNNASYG
                     KPAYIALYRRQLTELLTSYGPVFEVWFDGANGGSGYYGGARETRTIDKRTYYDWPGTF
                     ALVRKLQPGAVIFSDVGPDVRWVGDEKGIAGETCWATYDPVGEDGGPAAPGNVRASES
                     MQGTRFGSHWLPAECDVSIRPGWFWHERENDKVKTPQELVDLYFKSVGRGATLLLNIP
                     PNRHGQLSPQDVSSLESFHRHIQSIFHEDLAARARLQASNIRGQSHRYGPQFLLDEDP
                     GTFWATDDNVHTPELTVDFGSPCTFNVVRLREAIQLGQRIGAIALDAWQQGSWTQIAQ
                     ATSIGSCRLIRLQNAIETPRLRLRITDSPACIALSSLGVYLDQK"
     gene            13182..13955
                     /locus_tag="VFA02_01285"
     CDS             13182..13955
                     /locus_tag="VFA02_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZU08508.1"
                     /translation="MPEYSIVIPAYNESARIGNTLHEVLECVHRHNWNAEILVVNDGS
                     RDNTAAIIESFAAKDPMIRLIQNPGNRGKGFSVRNGMLHASGDIVMFTDADLSAPMDE
                     AERLFAAIREGADIAIGSRWLERRRQTIQQPLYRQFFGRCFNAVTRLIMRLPFADTQC
                     GFKAFRRQAAQTIFQLQQIERWGFDPEILFIALKRGFTVREVPVTWGHDERTRISYLK
                     DGVKMLQELVFIRWNAMTGVYDRPVEELAPPVAVAKGHR"
BASE COUNT         2967 a         3598 c         4539 g         3209 t
ORIGIN      
        1 aggaagccgg gtagttcgct gaggccggga actccgctga cggccgcgct ttgttgcttt
       61 gtcagattgc tgacgagaag cgcactttct ccctgggcca gagtgaccac tccggtgtat
      121 tggcgattgc tcaggatggg attgccgtcg agtgtctgtc cggtcaggga agtgattttc
      181 aggtcgagtt tgagtgatac gtcatggtct tgctggatac gcggcgtagt ggtgagcgtc
      241 agtccgaggt cctgatattg cacctggggg atggtggtat tgagactgct ctccagagat
      301 gcggaattga tgccgaggtt ttgaaggtag cttgagattc ctgcctgaga aattcctgga
      361 atgcccagag aggtgcctgc gagattcgag taagtggatg taacgatcgg ataacgtgtg
      421 ccgctgcgaa aggtcgcctc agcctggtcc tgcacgcgga gctgcacgtt gtctatttcg
      481 cgcgtatcgg aggaattcaa gctgagattg agcgttgcac tctggtcaag cgtgatgccg
      541 ctctgggtaa tgccgccgcc gaagagggcg aagggttgat tgaagaccgt tcccgagacg
      601 gcacccgatg ctatcaggat ggcgacgatg gcgagattgt cgcccggatt ggcaagcccg
      661 gaagcaataa tctgctgtac cgcgctttga ttttgctgaa tgatctgatt gacttccgag
      721 gggacattaa agacggttgt ctgctgaggc agttgcgcgc cgagattcac catgcgcgac
      781 tggtctattt cataaagccg gagattgagc aggacctgac cacgccctga gagcaactcg
      841 gccagagtgt ggttcagggc ctcaatgcgc tcctgggggc cgcgaacagt aagcgtgtgc
      901 tgctcctgct gtacggttgc ctgctggact tcaaagacct ggcgcacagc gttgccaatt
      961 tcagtggctt catctgtggt aaggccggga agatagacgg tttccattac ctgacgctcg
     1021 aattttgtgc gattttcctt tgtgtccgcg gcgacgagca cgcgtttttc gtcgagcgga
     1081 acgaagaaag tccccgtagc aagcttgagc atttgcgcgg cctgggcgaa gtccacatca
     1141 tctacatcaa agcggacaag gggggtaggt acagacgcat cgagcgtagg aatgatgcca
     1201 tacgcatgga gtacctgccg caggacttca gtcgctgaac tgcgcagatg aaagctcaat
     1261 tttccggggt tgggagcgag ctggacgggc ggcgcggcct gctcaacggc gggcatgatg
     1321 acggcatcat gcggtccatt gtcgcgcgca agctcatcca ggtgctgggc gacgatggga
     1381 ttttgcgggt cgagcgtgta agcctcggcc agatcggcac gcgcggccgc atcgttgccg
     1441 aggagccggg ccttgcctgc ttcctgaaca agctttgtga gcagatgctc tttggcgatg
     1501 atgaaggaag tggagtagtc gcggttttcc ggatcgagct gcgcagcgcg ctggaaatct
     1561 ttgaccgcgg cgcggaggtc gtcttttttg agggcctttg cgccttcaag aaatgccctg
     1621 gtggcggctg ccggatcgcg ctttacagcg ggctgctgcg catggctgct tatgccggcc
     1681 agcgtaagcg cggcaagaga tacccagtga tggcggcagc ggcgaatcat cgggcactcg
     1741 caagcaggtc agccagagtg gtgacacaga cttccggctg gtggaccaga aggtcgtcga
     1801 agctgtaatg tccggtggcg acggcgatgg tgggcaggtg gttggctttt gccgctgcaa
     1861 tgtcgaatgg agtatcaccc acaacacaga cggtcgcctc agggccggcg atggagcgcg
     1921 ccagagatgc ggcatgcgcg atcatgtcgg cgcgtttggc gaagcggtcg ctgaagccgc
     1981 cgaaggtgaa ccattggcgc acgccgatga cttccaactt gacccagccg atggcttcca
     2041 gattgccggt accaactcca aggatggccc cctggttgtg cagccatgcg agtatctcgt
     2101 ctactccggg cattttgacc agatccatct gttcgcgccg ggcggccaca agggagcaca
     2161 tatgcctgaa gacagcatcg agatggggtt cccagtggat ggggtcaagc tcggcgaggc
     2221 ggaaggcatc atgcaaaatg ccggggtcgg tgttgccgct aagggtgacg ccgtcaagga
     2281 caagctcgcg ccccattacg ctgcggacgc tttcaaagaa tgaatcgtaa tggatgcggt
     2341 cgcggctgcg cagcagcgtg ccatcaatgt cgaagagata ggcgttctgc cggtcccagc
     2401 ggaagccgtc ctgaatgcag atgcgtggat ttgccggcgc agaagcgggt gcagtgctca
     2461 acgaagcggt tgctccttag aaagtgtctg ctttcagtaa aacagaaagg cacacatccc
     2521 tgcgagagga gggcgctccg ggcgcttccg gatcaggagg tcacgccgaa gcgtgtggtc
     2581 ccttgtttta cttcaaatac aggacgttcg ctgcgcggcg ggcgcttgag tccgaggcga
     2641 atttgtgcag tatcaatttc aagctcggcg agggtgcggc tgagggtgtt gcggtgcata
     2701 cctagttctt ttgcggcttt gcactggttt cctttgtggt gcgcaagtac tgttgtgagg
     2761 taacggcgct taaattcgcg cacagcttca gcgtaagtga tgccacccgc gtgcatcgcg
     2821 gccacaagat tgtccagttc gcgtttcaca aatgcctctc taacgccgga tgtttttccg
     2881 gctgtttatg cctgtggctt cagccagtcg ggctgcgcat cacggatcag ctttactcca
     2941 tctctgatcc gctggcttaa ctctgccgga gtgacaatgg ccgcttcatg cgcggcggac
     3001 agaaaataag gcgcaagcct tgatttttca atccacgcgg cgtggggaag aaaagccgcc
     3061 acggccatga tacccagcgt taccaggaca caacccttca gaagaccgaa ggccgcgcct
     3121 atcaggcggt cagcccaccc aagtcctatt gtccggacgg accagcgaat gaatcgccca
     3181 gtcaacccgg cagcaaccat cactgcaaca gcaattgcta tgaaggcaat tgcttcggcc
     3241 gctccagagg aatgcaccca ggtttcaatc caggggagca gcttttgata attccagctt
     3301 gcaaggagta ctccaaggac cactccggca agcgagaagg cctcaacaaa aaagccatgt
     3361 ttcgccgcgc ttagtacgga aacgatcagg atgatgacaa tcgcccaatc tatcaacgcc
     3421 atgcgcggtc tattccttgc ttcaacccgg tctctacgag accaggggca ctctattttt
     3481 tctttcccaa cgcaccggca taagccagtg gccctgcaca cttacagtgt caatgttttt
     3541 ccagtgagca actcataggc ctggaagtat ttttcctgcg tttttttcac tacatcgtct
     3601 ggcaagccgg gcgcaggggc ctgcttgttc cagcgaatac tttcgagata gtcacgcaca
     3661 tactgcttgt cgaaggaagg ctgggcgcca ccaggcttgt aaccttcgcg cggccaaaaa
     3721 cgtgaggaat ccggcgtaag cacctcgtcg gcgagaatga tgccttcggc agtcgtgcca
     3781 aattcaaact tggtgtcggc aaggatgagt ccacggcttt ctgcgtgctc tgacgctttg
     3841 cgataaatgg caaggctgag atcgcggagc ttttccgcgg agcgcgcgcc gacgagagcg
     3901 accatttcat cgaacgaaat attttcatcg tgctttccgt ggaggctctt ggttgcagga
     3961 gtaaagaccg gttcgggcag tttgtctgac tcccggagac cctgaggcaa acgaatgccg
     4021 caaacagttc cggttgcctg atagtctttc cagcctgaac cggagagata gccgcgcacg
     4081 acgcactcga cggggaacat ctgtgcgcgc ttcacgagca tggagcggcc gcgcaactcg
     4141 tctttgcaac ctgccagctc acgggggtac tcgtccacgt ttgcagtgac gagatgattg
     4201 ggaacgatgt ccctgaggaa gtcgaaccag aagagcgata tctgtgtaag gaccttacct
     4261 ttgccgggga ttccggtggc aagcacatgg tcgaatgccg aaatgcggtc ggttgccacc
     4321 aggagcagaa actcacctac ggagtagagg tcgcgcactt ttccgcgagc atgcagaaca
     4381 agatcactga actcggtttc aagaagcgct ggcaattggg aaaaaccttt cacagagagg
     4441 ctgaaagttg attctgcttc tgtcagaaaa gtttgtcaac gcgtgatcaa tgtggatttc
     4501 acgcggagtc agcgcgtcat gccaccagcg aggggcggct gtcaagagga aatttctacg
     4561 ggggccatat gcagatacaa taagaagagg gccaatctgc ccgtagagag ccatcaaaga
     4621 catgtacgaa gtgactgtag agcgcgggtt ttcttccgga cattacctgc gtaattacaa
     4681 aggcaagtgc gagaacccgc acggccataa ttacaaagtc cgcattacgc tgcgcggcga
     4741 gaagctggac gcggcggggc tgctgctgga ttttaaagac ctgaaacacg tgatgcgtcc
     4801 ggtgatcgag cggcttgatc accagatgct gaatgacctg gagccgttta ccgagatcaa
     4861 tccttcggcg gagaacctgg cccgttattt ttttgacgag acaaacaggc agcttgcgga
     4921 gatgaccgga gggcgcgtgc gcgtgaaaga ctgcacaatc tacgagaccg acaccacaac
     4981 tgcgacctac tacgagtaac tgtctgtgta tctgattgag atttataaat cagtgcaggg
     5041 cgaatcgagt tttgccggtc gcccgtgcat ctttgtgcgg cttgcggggt gtaatctgcg
     5101 ctgctcgtgg tgcgattcag aatacacctt caagggcggt tacaagctgt cagaggaaga
     5161 agttgttgct gagatcgaga agctggcccc ggtcaagcta attgaattta ccggcggcga
     5221 accactgctg caggagcggg agcttgttcc gctgatggag cggctgctgg ccaatggtta
     5281 cgaactgatg attgagacga gcggcgagcg gcccctggag cgcgttcctg ccggggtaca
     5341 caaaattgtg gacgtgaagt gtcctgcatc gggcgaaggc ggcagtttca ggatgagtaa
     5401 tcttgaattt ctgacccagg gcgacgaggt gaaatttgtg attgccgatc gcggcgatta
     5461 tgaatttgcc cgcgacttca tccggcagca cgcgcttgag gtacgtgtgg gacatgtcct
     5521 gctttcgccc gcattcaata aagcagcttc aacggaacgc agcacggcaa actgtttgct
     5581 ggatccgcgg gaactggtgg agtggatgct tgccgacggg ctggatgcgc ggatgagtct
     5641 gcagatccat aagttcatct gggagccgca gaagaagggc gtttgaaggc ggcggtgcgc
     5701 gagtgaatgt ttgccggtct tactgcggct gggtttgcag atacttgtcg aagggaatct
     5761 cgaaatagaa aagctctttt ccgtcggtat cttttagttg ccgcagaagc agttttgctc
     5821 cgtggagggg agacgacccc agtccctgaa tgtcataaaa gagaaagcct gctcgtgtgg
     5881 tatgcggctc gacgacgagt gcctgatatt cgaattcaga aaaatcctgc tcaactttgc
     5941 tgttcggcgt tttgggtttg gtatgcagcc ggatggggcc gacgggaata tcggcgcctt
     6001 ttttgtcgcg cagagagatg cggcgttcga tatcatccgg ttcggcggca ggaatacggt
     6061 cacctgcggc gctgatgaag taaatccggg cctggtcgag cgagatgggc ctgtctccgc
     6121 tattggtgat gatgatacgg atgggcagga actggtggtc aaggtaattc acgcggaaga
     6181 atttgatttt ttccgctgtg ttatacggct cggcggcgat ggccacatgc tcgtccggat
     6241 gcacgtcgac cgcggggtat gtggttgcgt cctttgccgg aggcgggcca tgttcaaatg
     6301 ccggagacgc tggcacacaa agggccagca aaacaattga cagcagagaa cgcagacgca
     6361 tcattctgcc gaccattatc atcgttgatt aagacttcgc aaagcaggtt ttggtaagct
     6421 gatgattttt tacagcctgg gtctggccct gacgcttgta ttgggggcgc cgtactggct
     6481 cttccgcatg gccgccagcg ggaaataccg tgaagggctg ggcgagcggt ttgggcgcgt
     6541 accggagcgg ctgctgcatg gtgatgggcg gcctgtgatc tgggtccatg cggtttcagt
     6601 gggggaactg ctggcggcct cgcggatggt gctggaactg cgggagagga tgccggaatg
     6661 gcgcgttgtg gtctcgacca caacgagaac agggcaggcg ctggcgcgag agcggttcgg
     6721 gaatgagaac gtcttttatt tcccactgga ctttgcgttt gctgtgcgca ggtatttgcg
     6781 cgcgctgaat ccgcgcatgc tggtgttgat ggagactgag ttctggccgc gtttgctgat
     6841 ggaatgccgg cgggcgcgca ttccggtcgc agtggtgaat gcccggatat ccgaccgctc
     6901 gtggccgcgt tatttccggc tgcggttttt ctggaaacag atgctgaatg gccttgctct
     6961 ggtgctggcg cagagcgagc tggacgtgga aaggctgcgc gcaattgggg cggcaaatgt
     7021 tcgctacgga ggaaatctga agtatgacat tcgcacggtt gtgcctgctg ctgtaacgca
     7081 aaagctgaaa gaattgatcg cggcaggaac gaaggtcctg gtttgcggaa gtacgctgga
     7141 aggtgaagag aaggccctgc tggaggccgc gccttccgat gctctggttg tccttgcgcc
     7201 gcgtcatccc gagcgctttg aagctgtggc gcagttgctg aagcagagcg gcagaccgtg
     7261 ggtgcggcgg tcgcagtgga tggagcaacc gaagccgctg cggggaggag atattttcct
     7321 gctggattcg atgggtgagc ttgcgtcagt gtattcgctg gcgacggttg catggatcgg
     7381 cggaagcctg gtgccggcag gaggacataa tcctctggaa gcggcgcagt ttcgcgtgcc
     7441 cgtggtgatg gggccgcatt atgaaaactt tcgcgctatt gtggacaggc tgcgcgcggc
     7501 ggatgcgatt cggattgcaa atcccaatga ggtgggcagt gtattggcat ctctgctcag
     7561 cgacagggaa ttggcgcata acattggtga gcgagcatac cggattttta ccgcggaatc
     7621 gggagccact gtacgcgcgg cagagtcgtt gctggagctg gccggaggca aggcctgatg
     7681 cgccgtctgt tatggccttt gaatcctgtg tatgccgctt cagtggcggc aaagaatgtt
     7741 gcgtatgatc gcggatggct taagacgaag cggacgcaat ggcctgttgt gagtgttggc
     7801 aatctttcag ccggcgggtc gggcaagact ccttttgtga ttgctctcgc gagactgctt
     7861 gtgcgaaatg gcgtacaggc agatgtgctt tcgcggggat acgggcgcag tgcagatgcg
     7921 attgaaaaag taaacccttc cggcgatgca gcgcggtccg gagatgagcc gctgcttatt
     7981 gcgcagtctg cgggtgttcc tgtgtacgtg ggcgcagacc ggtatgaggc agggcttgtg
     8041 gctgaaaaga atgagagcgg cccgcggatc catctgctgg atgatggttt tcaacacagg
     8101 cggctagcgc gtgatctgga tattgtattg cttcaccgca ccgatttttc tgagagcctt
     8161 ttgcctgtcg ggaacctgcg tgagccgctg tccgcgttac ggcgggcttc ggcggttgtt
     8221 cttcgccagg aagatgccgc attcgaggag aacctgagag agcggggaat tgaagcgcct
     8281 gtctggctca tccggcgcgt catgaatgta cccttcgacg ccggcagggt ggttgcattc
     8341 tgtgggattg cgcggcccgg ggagtttttt tcgctgctac gcgcgaatgg cgctgaagtt
     8401 gcggacacat tggcctttcg cgaccatcac cgttataccg aagatgacct gaaacggttg
     8461 attggacttg caaagtcggc gaaagcagat gcttttctta cgactgagaa ggactctgta
     8521 cggttgcccc aggaaatgaa ggagcgcctg agtgcagttg ctccgctgag gctggtgcca
     8581 ttggagacaa ggttcgagga catgagtgcg gtattgaaga aggtcctgcc tcttgcggga
     8641 tgatgcgagg gcgcctttat gtgtggccgg agttggcaat cgacactgtg agtggatggt
     8701 caatcatgag aaaatcaaag tttgccgata aagactcagc aattgagggt gctcatcgta
     8761 aggctgggcg cgatggggga cattttgcac gcccttccag cggtcacggc attgcgtgcg
     8821 gcgcatccgg agtggttttt gggatgggcg gttgagccgc agtggcgcgg actgcttgcc
     8881 gcccatggag ctactgcacg cgggccggag atgccagtcg tagaccagat ccatgttgtt
     8941 ccagcaaaac aatgggggcg aaatccgctg catgtgagta cggtcgagga cattcggcgg
     9001 ttgcggcgcg aatttcgtgc tgcccgctac gacgtctgtg tggatttgca gggggctgta
     9061 cggtcggcgg tggtggggaa atgggcgcat ccgcaacggc tgatcgggga ggcgataccg
     9121 cgcgaatggg cggcgcaata cctgtttgac gagcgggtcg agacgcatgg tgttcatgtg
     9181 attgagcagg caatcgaggt ggcaaatgag gttgcagggg aaagccttcc ggtaatgcag
     9241 ccgtggcttc cgctgagtga aagcgcggag cgcagggccg ctgaatttca tgggccactg
     9301 gtcatcctca atcccggagc aggatggggg gcgaagcgct ggcctgcaga acggtacggc
     9361 gcagtggcgc gtgtactggc aggaaaggga tttcaagtgg tcatcaacgt tgggccggga
     9421 gaagagcagt tggcgcagga agtgagcggg gcaagcggag gcgtgtccct gccggtttca
     9481 ccgcatctcg atgaactgat tgcgctcacg cggcgagcat cgctggtgat tgcaggagac
     9541 accgggccgc tgcatctggc ttcagctttg ggtattccgg tggtcggcat ttatgggcca
     9601 actgacccgg cgcggaatgg gccgtttggc ggaaaattca gggttttgcg gcatcctgag
     9661 agcaaacgcg accactcacg ccgtacagag ccggaggctg gtttactgac gatttcagca
     9721 gaagacgtgc ttgcggccgc catagacctt cttgaggaga atgcctcgaa tggccgctga
     9781 ctggagcagg attgcgcgac gcatccgcgt gccgctgggg tttgtctttg ccgtggttta
     9841 catcctgctg gcgcggccca catttgtttc catgatgtgg agtctgctgc tggttcttcc
     9901 ggggctctgg ctgcgcgctt acgcttcagg atatgtgaag aaaaacgcag agttgactgt
     9961 aaccgggcct tacgcctata cgcggaaccc gttgtatctc ggctccatga tgatcgcgtt
    10021 tggatttgcg gaggcgtcca gaagcattgt gaccgttctg ctgctggctg tgctgtttgc
    10081 ggtgatttat cttcccgtca tcgggtcaga agaacggttt ctgcgcagca cattcaaggg
    10141 ctttgacgag tatgcagcga aagtcccacg gttgttaccg cgcttgcggc ctgcgcgact
    10201 gttgcaaaat ggggcaggag agttctcttt cgacctttac aggaaacacc gcgagtacaa
    10261 tgcactcatg ggagcggcgg ccatctatgc tgtgcttgcc ctcagaatta tctattttca
    10321 ttaagagtga gcacttattt tgacctggcg ttttcgattg gttcccgcag ttttgtgttt
    10381 tttgttgctt cctggcggaa aggcgcagtc tccacaaggc cttccatcgt tgccagcgcc
    10441 gcatcccgga ctagggcttc cggcacggca gacgctgaat tacagcgtag actggcgtgt
    10501 ttttccggcg ggcacggtca gttttcatca ggaagccgaa ggcgacgcac agcacattac
    10561 ggccgtcgcc gattccattg gcgcggtcaa cctgatttat cgtgtaagcg accgctttca
    10621 gtcctcgttt agccgccgga cgggatgctc cactgcgttc agtaagcaac ttcaggaagg
    10681 ccgccggcag gtgaacagca ctctgaactt caattacggg cagggcaagg ccatccttga
    10741 ggaaaagaac ctggtttccg gaattgcgaa acatcaggaa gccccggtgc cgtcctgtgt
    10801 aagcgacctg ctctccgcga ttttctacac ggcatcccag ccgttgcagg tggggcagag
    10861 tttccagatt ccggtgtatg acgcgatgcg gaccataccg gtcacgatga aggtggagag
    10921 cagggaatct gttcgtactc cttccggtac atatcagacg gttcgcgtac agccgactgc
    10981 cgatgccgga gtggtgaaga accgggggaa tatctggatc tggtatacgg acgatgagcg
    11041 gcacattcct gtgcagatgc gcgcacgtct cttctgggga acgctgacgt tccagttgac
    11101 gggcatagag cagaaataag ggctattgga tccggtagcg ttcgcgcatc aggcgctgaa
    11161 gacgcgggcg gtagatgatg tcccaggcaa gctcctttcc ggggaacatg gcaagcgctg
    11221 cgcgtcgagt gtcggcgacc atctcggagg cttcgtctat ggtgagagtg ttgtcctggc
    11281 tgatgacgga catgaccatg ttcatcatga tctgcaaacg gcggattttt cgctgctctt
    11341 cctgaatttc ctctggtgtc tgctgagagg aatcaaagtg cgactgctga aactcagggg
    11401 aattgccagc ttcactcagg cccatggtgc atcctccgta gggggcgtgc tttttcgaca
    11461 tcctacaacg gtagcgatgc gccggaaata gaaaaagatt ccctcaggtg cctgagttgg
    11521 cacttgccag cagggacaga gactttattt ttggtccagg tacactccca ggctggacag
    11581 agcaatgcag gcgggggaat cggtaatgcg caggcgcagg cggggagttt cgatagcatt
    11641 ttgcagcctg atcagacggc aagagccgat gctggttgcc tgagcaattt gtgtccacga
    11701 accctgctgc caggcatcga gcgcgatggc gccaatgcgc tggcccaact ggattgcttc
    11761 ccgcagccgc accacgttga acgtgcatgg actgccgaaa tccacagtca gctcaggtgt
    11821 gtgtacattg tcatcggtgg cccagaacgt accggggtct tcatcgagca ggaactgagg
    11881 gccatagcgg tgactctgcc cgcgtatgtt cgaggcctgc agacgcgccc tggcggcaag
    11941 gtcttcgtga aagatgcttt gtatgtggcg gtggaacgat tcgagagagg acacgtcctg
    12001 cggtgatagc tggccgtggc gattgggcgg gatattcagc agaagggttg cgccccttcc
    12061 aaccgacttg aaatagagat ccacgagttc ctgcggggtc ttgaccttat cgttctcacg
    12121 ctcgtgccag aaccagcctg ggcggatgga gacgtcgcat tcggccggca accaatgcga
    12181 gccgaagcga gtgccttgca tggattcgct ggcgcgcaca tttccgggcg cagccggtcc
    12241 tccgtcttcg cctacggggt cgtacgtggc ccagcaggtt tcgccggcta tacctttttc
    12301 gtcgccaacc cagcggacgt ccgggcctac atcgctgaag ataacggcac cgggctggag
    12361 tttgcgtacc aaggcaaaag tgccgggcca atcgtagtaa gtacgcttat caatggtgcg
    12421 ggtctctctg gcgccgccgt aatagccgct tccaccattg gcgccatcga accagacttc
    12481 aaagacaggg ccatacgatg tcagcaattc ggtgagctgc cgacgataca aagcaatata
    12541 cgcaggtttg ccgtagctgg cattgtttct gtcccagggg gagcaataga tcccgaactt
    12601 cagaccatgt ctctgcgcgg cctgggaaat gtcgcggaca atatcgcctt tgccattttt
    12661 ccaagggctg ttgcggatgc agtgatcggt ggtctttgta ggccagaggc agaaaccatc
    12721 gtgatgcttg caggtgagga tgacgccttt catgccgcct gccttgaggg cggaaacaat
    12781 ggcatcggga tcaaaaccct ggggattgaa gatggacggg tcttcatctc catcgcccca
    12841 ttccttgtca gtgaaggtgt tgactgtgaa atgaaggaat gcgttggttt ccatgcgttg
    12901 ccagcgcaac tggcgggcgg aaggcaaggc cccgaagggg gctggtggag gaacggcttc
    12961 tccagaagca tataagctgc cgaaggtcag tgctgaggtg accagaaaat tccgcctggt
    13021 tactgtcatg ccagagtctt tcctttcctg atggagcttc gctttttgat tctatattcg
    13081 aattcctgat gggaatgatt ccaagcgggg aagcatgttt ctatgaaaac tatgtaaatc
    13141 cggaatatga ttacgaagga aggctgcgcg atttttaagt gatccccgaa tacagtatcg
    13201 ttattccggc ctacaatgaa agcgcccgga ttggaaatac gctgcatgag gtcttagagt
    13261 gtgtccacag gcacaactgg aacgccgaaa tactggttgt gaatgatggt tcgcgcgaca
    13321 atacagccgc gattattgaa tcttttgcgg caaaagaccc gatgataagg ctgattcaga
    13381 accccggaaa ccgggggaaa gggttcagcg tgcgcaatgg aatgctccat gcctcaggcg
    13441 acattgtgat gttcacagac gccgaccttt ctgctcctat ggacgaggcg gagcggctgt
    13501 ttgccgccat tcgggaaggt gccgacattg ccatcggctc gcgttggctg gaacgcaggc
    13561 ggcaaaccat ccagcagccg ctctaccgcc agttctttgg ccgctgcttc aatgcagtta
    13621 cgcggcttat catgagactg ccatttgccg atacccaatg cggcttcaag gcgtttcgga
    13681 gacaggcagc gcagacgatc ttccaattgc agcagattga gcgctggggg ttcgatccgg
    13741 agattctgtt tattgcgctc aagagaggat ttaccgtgcg cgaggtcccg gtaacttggg
    13801 gacatgacga gcgtacaaga atcagctacc tgaaagatgg ggtaaagatg ctgcaggagc
    13861 tggtgtttat tcgctggaat gctatgaccg gggtctacga ccgtccggta gaagagcttg
    13921 cgccgccggt tgctgtcgca aagggtcacc gatagacaaa agaaaagcat ccctctgttc
    13981 gggacgcttt tctgaatttc ctgggccctt cttcaggaag ccaacgccat gacttccgga
    14041 ttccggacca gtgcaggtcc tacgcggcgt ggcaccaggc gaatccccga tccggcttcc
    14101 cgttcgatca tgaagcgctc agaacatggg ccgcaaagcc agtaatgttc caaacggcgg
    14161 ggtgcaccgt cagtttttcc cgttatgtcg aagacaaaga tcctgccttc gcgcaggtaa
    14221 tgtagcggct ttgcgcactc agggtttgcg caattattta ccataaccat ctccccgaaa
    14281 aactgctgcg ctcatgggct gcggcggatg agc
//
LOCUS       DASRBV010000029         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_1364,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000029 DASRBV010000000
VERSION     DASRBV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3304
                     /locus_tag="VFA02_01290"
     CDS             <1..3304
                     /locus_tag="VFA02_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08509.1"
                     /translation="FEVQHVRVGVAAHVGVNAKLEVGKSTNTVTVSAGKAARLDTQDA
                     VLGFTVESRSAMDLPILYSNPFELQLLAPAVISTSLSTGNHTYEGGSESSTVDGSQSG
                     RTEFTLDGAPDTRNGGAVTTAYIPSRDFIGEFKLITSPYDASLSHTSGASLDTSIKSG
                     TGQFHGNASVFYQDPNVDAPQFSQGPAVAPAIKYHRESIGVGGPVFPRRLFFFAGYEH
                     QYNLQAASTTTQTVPTPAEKQGDFSALLGIGKTQTAKYTCPSTHKTITTAPFNQYQIF
                     DPYTTKPDPNCPGLFVRQPVPGNILTNMDPVAKKILGYYPDPTGSATEQPNGQNNFVS
                     NAANVDRYWDVLARLDYNINNNQKVFGHFIISDRVQPGKNLFFPGASGKTNILKNKGG
                     VLDYVNTLNNTTLLNLRYSVTRFYTSATIDAKTTATQLGINPNATAGVPAVASGFPYV
                     SISGYAELGNADPSFEADTIHDAQVNLSKGLGAHQLRAGAEWRMYQANQADFTNEKLF
                     IQSKGTYTLGPSSALAASPLGQALAGFEFGIAEGTQERLNAQTANNTTYWSGYVQDDW
                     KVDPVLTLNLGVRYEYFGPVVERNGKSITYFDTSVPSPIAAQATANYATMATPSEQAL
                     VPVSQFAVNGGVRFASPGQGLWHSQTGNFSPRFGFAWNPVTPLVVRGGFGLFYQHLGE
                     YVQYGNPLGFTQTTNSIVTLDNGQTYQATLENPFPSGLVQPSGSSFGLLQNIGQSISQ
                     FFPQHPKNPMNERFSFGFQYALPAEIVLSADYVGSLGRHLRITRDFDGVPLRYLSRSP
                     VRDASMNAINAQLNAAYPNPFQGIPVPGSSSLTGSTISGSQLVKPFPHFTGLTAGDES
                     GMSNYNGLQVSAQKRFSHGYNMSVSYTWSRTLDALSFLNPADARPWYGTSNTDYPQVL
                     SVAGIYELPFGRGRMFFSSAPRWLDEIINGFQVEGTYRIQSGQPLTFNSSGTVLKPGA
                     KYSDIHGPAAHSISQWFNTHAIENYQDATAEGLNYGNDLALVSNVRTFPLRFNNVRQD
                     YQNLLNVGAMKKFTVFQERVHMDLRAEAINAMNHQVFAAPNTDPSSASFGKIGGPGNT
                     SRQLQFAVECQF"
     gene            complement(3376..5364)
                     /gene="uvrB"
                     /locus_tag="VFA02_01295"
     CDS             complement(3376..5364)
                     /gene="uvrB"
                     /locus_tag="VFA02_01295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZU08510.1"
                     /translation="MDFQLVSDYKPRGDQGRAIDELVSGIHAGEKHQVLLGVTGSGKT
                     FTMAKVIEELNRPVLVLAHNKTLAAQLYHEFKQFFPNNAVEYFVSYYDYYQPEAYIPS
                     GDLYIEKEATINEELDKLRLSATRSLFERRDVVIVSSVSCIYGLGDPDAYYGMLLLLE
                     KGQRVRREDITRKLVEILYERNDADFRRGTFRVRGDVIEVFPTYDENAYRIELFGDEV
                     DSLSQIDPLFGTVKQKYARLPVYPKSHYVVQPQRRQAAIDSILEELAWWEKELESQGR
                     LVESQRVHQRTRFDLEMIKSVGYCHGIENYSRHFSGRLPGEPPPTLLDYFPRDYLIFI
                     DESHATIPQLHGMWHGDRSRKQNLVDYGFRLPSAMDNRPLRFEEFEARTNQLVYVSAT
                     PGPYELTKSSGVVVEQIIRPTGLIDPEVEVRPVRGQIDDLLAEIRDRAKKNERVLVTT
                     LTKRMAEDLAGYYTEVGVRCRYMHSEIETLERVRLLRDLRKGEYDVLIGINLLREGLD
                     LPEVSLVAILDADKEGFLRSTGSLIQTIGRAARHVNGKAILYADNITDSMRQAMDETS
                     RRREIQRAYNEEHGITPTSVIRAADMSLAQILKADYADLTEEADEMPEFRSQEELDVY
                     ILKLENEMREAAKVFEFEKAAKLRDTIKELRTKEFLFS"
     gene            complement(5385..>6683)
                     /locus_tag="VFA02_01300"
     CDS             complement(5385..>6683)
                     /locus_tag="VFA02_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU08511.1"
                     /translation="FFCGIAPSLPLLIFARVLQGAGGGGLAPSEQAILADTFPVSKRG
                     QAFAVYGMAVVVAPAIGPTLGGWITDNFNWHWIFFINIPFGILSLFLSQRMVEDPPYL
                     KEEQKKTRGVDSVGLGLICVGVGCLEFVLDKGQEKDWFGDRMITGFFIAALCALVAFV
                     FWEWNHEDPVVDIKLLRVRNFGTAIFLQFILGMVLFGSTVLIPQFLQVLMGYTAERAG
                     MALSPGALVLIFMMPIAGRLITKMDARLLVSIGYAITAIGLYNLTRLDLETSFGTIIL
                     WRMFQVCGLAFVFIPISTLNYVGVPFNKNNQISSFSNFARNIGGSVGTALLTTFLQRN
                     AQAHQAVLGANVIAGSQRYMNFVEATKQSLMRLGQSSDQAAQTAVGHAYQVLLQQSSM
                     LSYVNAFWGLSIIIACLVPLPFIMRKPPKMQKPAAEAAGH"
BASE COUNT         1482 a         1795 c         1966 g         1440 t
ORIGIN      
        1 atttgaggtg cagcatgtcc gtgtgggtgt tgcggcgcat gtgggcgtaa atgcgaagct
       61 cgaggtgggc aagagcacca acaccgtgac tgtgagtgca ggtaaggcgg cgcggctgga
      121 cacgcaggac gcggtcctgg gctttacggt ggaatcgcgt tcggcgatgg accttccgat
      181 tctttacagc aatccctttg agctgcaact gctggcacct gcggtgatca gcacgtcgtt
      241 gtcaacgggc aatcatacgt atgaaggcgg gtcggaaagc tcaaccgtgg acggctcgca
      301 gagcgggcgg accgagttca cgctggacgg cgccccggac acacgcaatg gcggtgcggt
      361 gacgacggcc tatattccat cgcgtgattt tatcggggaa ttcaagttga ttacatcgcc
      421 gtatgatgct tcgctttcgc atacgtccgg ggcatcgctg gacacaagca tcaaatcggg
      481 caccgggcag ttccacggaa atgcgagtgt gttttatcag gaccccaatg tggatgctcc
      541 gcagttttcg caagggcctg ctgttgctcc tgcgattaaa taccaccgcg agagcattgg
      601 cgtcggcggg cctgtttttc cgcgcagact cttctttttt gccggatatg agcaccaata
      661 caacctgcag gcggcgagca cgacgacgca gacagtgccg acgccggctg aaaagcaggg
      721 agatttttct gccctgctgg ggataggcaa gacacagacg gcgaagtaca cgtgtccttc
      781 cacacataag acgatcacga cggcgccgtt taatcagtat cagatttttg atccgtacac
      841 gacaaagcct gacccgaact gtccggggct gtttgtgcgc cagcctgtgc cgggcaacat
      901 tctcacgaac atggaccccg tggcgaagaa gatactgggc tattatccgg acccaacagg
      961 ttcagcgacg gagcagccga acgggcagaa caactttgtt tccaacgcgg cgaatgtgga
     1021 ccgctactgg gatgtgctgg cgcgtctgga ttacaacatc aacaacaatc agaaagtgtt
     1081 cgggcatttc atcatcagcg accgcgtgca gccgggcaag aacctgttct ttcccggcgc
     1141 gagcggcaag acgaacattc tgaaaaacaa gggcggcgtg ctggactatg taaacacgct
     1201 gaacaacacg accctgctga accttcgcta ctctgtcacg cgcttttaca ccagcgccac
     1261 gattgatgcc aaaacgacgg caacgcagct tggcatcaat ccaaatgcta cggcaggggt
     1321 cccggcagtg gcgtcaggat ttccatacgt gagcatcagt ggatatgcgg agcttggaaa
     1381 cgcggacccg agctttgaag ctgacaccat ccacgatgcg caggtaaatc tgagcaaggg
     1441 cctgggcgcg caccagctca gagcaggagc ggaatggcgc atgtatcagg ccaatcaggc
     1501 agactttacg aatgagaagc tgtttattca gtcgaaaggg acgtacacgc tggggccatc
     1561 cagcgcgctg gctgcttctc cgttaggaca ggcgctggca ggatttgagt ttggcattgc
     1621 tgagggaacg caggaaagat tgaatgcgca gactgccaac aataccacgt actggtcagg
     1681 atatgtgcag gacgactgga aggtggaccc ggtactgacg ctcaatctgg gtgtccggta
     1741 tgaatatttc gggcctgtcg ttgagcgcaa cggcaaaagc attacatatt ttgatacatc
     1801 tgtgcccagc ccgattgcgg cgcaggccac agcaaactat gcgacgatgg ccacgccatc
     1861 cgaacaggcg ctggtgcctg tatcccagtt tgccgtcaat ggcggcgtgc ggtttgcgtc
     1921 gcccggacag gggctctggc attcgcagac cgggaacttt tctccgcgat ttggttttgc
     1981 gtggaacccg gttacgccgc tggtggtgcg cggcgggttt gggcttttct atcagcacct
     2041 gggtgaatat gtgcaatatg gaaacccgct gggctttaca cagaccacaa acagtatcgt
     2101 tactctggac aatgggcaga cataccaggc gacgctggaa aatcccttcc cgagtgggct
     2161 ggtgcagcca tcaggtagct cgtttggcct gttacagaac atcgggcagt ctatttcgca
     2221 gttctttcca cagcacccca aaaatccgat gaatgagcgc ttctcctttg gctttcagta
     2281 cgcgttgccg gcagagatag tgctgagcgc agactatgtg ggcagcctgg gacgccatct
     2341 cagaattacc cgcgattttg atggagtgcc tttgcgatat ctgagccggt ctcctgtgcg
     2401 tgatgcctcg atgaacgcca tcaatgccca gttgaatgcg gcctatccga acccatttca
     2461 gggcatcccg gtgccggggt cgtcatcgct caccggctcg accatttccg gcagccagct
     2521 tgtaaagccg ttcccacatt tcacggggct gactgcgggc gatgaatcgg ggatgagtaa
     2581 ttacaacgga ttgcaggttt cagcgcagaa gcggttttct cacggatata acatgagcgt
     2641 gtcttataca tggtcgcgca cactggatgc gctctcgttc ctgaatccgg cggatgcaag
     2701 gccgtggtat gggacttcca acacggacta tcctcaggtg ctttccgtgg ctggaattta
     2761 tgaactgcct tttgggcgtg ggcgtatgtt cttctcgtcc gctccacgct ggctggatga
     2821 gatcatcaac ggcttccagg tagagggcac gtatcgcatt cagagcgggc agccgctcac
     2881 gttcaacagc tcagggacgg tgctcaaacc aggagcaaag tattcggaca ttcatggacc
     2941 ggcggcccac agcatctcac agtggtttaa cactcatgcg attgagaact atcaggacgc
     3001 aaccgcggaa gggttaaatt acggcaatga tctggcgctg gtgtcaaatg tgaggacctt
     3061 tccgctgcgc ttcaataatg tgcgacagga ctaccagaac cttctgaatg ttggcgcaat
     3121 gaagaagttc accgtatttc aggagcgtgt gcatatggac cttcgtgccg aggccatcaa
     3181 tgctatgaac catcaggtgt ttgccgcgcc gaatacggac ccgtccagcg cgagtttcgg
     3241 caagattggc ggccctggaa acacttcacg acagttgcag tttgccgtgg agtgccagtt
     3301 ctgacgccct gagagagtgg ggacaccgtt gtggtcaggc ccacatcttt tcaggatggg
     3361 ggcccctggc tttgactaac tgaacagaaa ttcttttgtg cgcagttcct tgatggtgtc
     3421 gcgcagtttt gcggccttct caaattcaaa gacctttgcg gcctcgcgca tctcgttttc
     3481 gagtttcagg atgtagacat ccagctcttc ctgcgacctg aattctggca tctcgtctgc
     3541 ttcttcggtc aggtcggcgt aatcggcctt gagaatctgg gccagagaca tatcggccgc
     3601 acgaatgacg gatgttggag taatgccgtg ttcctcgttg taggcgcgct ggatttcgcg
     3661 ccggcgtgaa gtttcgtcca tcgcctggcg catcgagtct gtgatgttgt ccgcgtagag
     3721 aatggctttg ccattgacgt gacgcgcggc acgtccgatg gtctgaatga gggacccggt
     3781 ggagcgaaga aagccttctt tatctgcgtc gagaatggcg acaagcgaaa cttcgggcag
     3841 gtcaagacct tcacgcagca gattgatgcc gatgagaacg tcgtattcgc ctttgcgaag
     3901 atcgcgcagc agacgcacgc gttccagcgt ttcgatttcc gagtgcatat agcggcagcg
     3961 cacgccgact tcggtgtagt accctgcgag gtcctcggcc atgcgtttgg tcagggttgt
     4021 gaccagcacc cgctcgttct ttttggcgcg gtcacggatt tccgcaagca ggtcatcaat
     4081 ctgtccgcgc acgggacgca cctccacttc cgggtcaatc agcccggtag gccggatgat
     4141 ctgctcgacg acaacgccgg aggacttggt cagctcatac ggtcccggag tggccgacac
     4201 ataaaccaac tggttggtgc gcgcctcgaa ctcttcaaag cgcaaaggtc tgttgtccat
     4261 cgcagaaggc agacggaatc cgtaatccac caggttctgc ttgcgcgagc ggtcaccgtg
     4321 ccacatgccg tgcaactgcg ggatcgtagc gtgcgattca tcaataaaaa tgagatagtc
     4381 gcgggggaag taatcgagca atgtgggagg aggctcgccg ggcagtcttc cggaaaagtg
     4441 gcgcgagtag ttttcgatgc cgtggcaata gccgacggac ttgatcattt caagatcaaa
     4501 ccgcgtccgc tgatgcacgc gctgcgattc gacgagacgg ccttgtgatt ccagctcttt
     4561 ttcccaccag gccagttctt ccagaatgga atcaatggcg gcctgccgtc tctgcggctg
     4621 tacgacgtag tggcttttgg gatagacagg cagacgcgcg tacttctgct tcacagtgcc
     4681 gaagagcgga tcaatctgcg agagggaatc tacttcatct ccgaaaagct caatgcgata
     4741 agcattttcg tcgtaagtcg ggaagacctc gataacatcg ccgcgcacgc ggaatgttcc
     4801 gcgccggaaa tcagcatcgt ttcgctcata gaggatttct accagcttgc gcgtgatgtc
     4861 ttcgcggcgc acgcgctggc ctttttcaag cagcagaagc attccgtagt aggcgtcagg
     4921 gtcgccgagg ccgtaaatgc aggagacgga agagacgatg accacatcgc ggcgttcaaa
     4981 aagcgagcgc gtggcggaaa gccgcagttt gtccagctct tcgttgatgg tggcttcttt
     5041 ttcaatgtaa aggtcgccgg agggaatata cgcttccggc tggtagtagt cgtaatacga
     5101 gacgaagtac tcgacggcat tgttggggaa aaactgcttg aactcgtgat aaagctgcgc
     5161 tgcaagcgtc ttattgtggg ccagcaccag cacaggacgg ttgagttcct cgatgacctt
     5221 cgccatagtg aaggtcttgc cggagcccgt cacaccgaga aggacctggt gcttttcgcc
     5281 ggcatggatg ccggagacaa gctcatcaat ggcgcgcccc tggtcgccgc gcggcttata
     5341 gtcgctcact agctggaaat ccatcttcgc ctcaagtgtt catgttaatg ccctgcggcc
     5401 tcagccgcag gtttctgcat ctttggcggc ttgcgcataa tgaacggcag cgggaccagg
     5461 cacgcaatga taatgctgag gccccagaag gcattcacat agctcagcat ggaggactgc
     5521 tggaggagca cctggtaggc atgtcccacg gccgtttgcg cggcctggtc agagctttgg
     5581 ccgagacgca tcagcgattg tttcgtggct tcaacaaaat tcatatagcg ctggctgcct
     5641 gcaattacat tggccccaag cactgcctgg tgcgcctgcg cgttgcgttg caggaaggtc
     5701 gtcagaagcg cagttcccac gctgccgccg atgttgcggg cgaagttaga gaagctggaa
     5761 atctggttgt tcttattgaa gggcactccg acgtagttca gcgtgctgat agggataaag
     5821 acaaacgcca gtccgcagac ctgaaacatg cgccagagaa tgatggtgcc gaagctcgtc
     5881 tcaaggtcaa gccgcgtcag gttgtacaag ccaatcgcag taatggcata gccaatggag
     5941 accagcagac gcgcatccat tttggtgatg aggcgtccgg caatcggcat catgaaaatc
     6001 agcaccaggg cgccgggaga aagcgccatg ccggcgcgct cggcggtgta gcccatcagc
     6061 acctgtaaga actgggggat gagaacagtg ctgccaaaca gtaccattcc caggatgaac
     6121 tgcaaaaata tggccgtgcc gaaattgcgc actcgcagca atttgatgtc taccaccgga
     6181 tcttcatggt tccactccca gaaaacgaag gccacgagcg cgcacagggc ggcaatgaag
     6241 aagccggtaa tcatgcggtc gccgaaccag tccttctcct ggcctttgtc gagcacgaac
     6301 tcaaggcatc cgacgccaac gcagatgagg cccagtccaa cggaatccac tcctctggtc
     6361 ttcttctgct cttctttcaa atagggagga tcttcgacca tgcgctgaga aagaaacagc
     6421 gagagtatcc cgaaaggaat gttgatgaaa aagatccagt gccagttgaa gttgtcagtg
     6481 atccatccgc cgagcgtcgg gccgatggcc ggggccacga ccaccgccat tccgtagaca
     6541 gcaaaagcct gtccccgctt gctgacggga aacgtatcgg cgaggatggc ctgttcgctg
     6601 ggggcgagtc cgcctccgcc tgcaccctgc aaaacgcgcg cgaagatcag cagagggaga
     6661 gatggcgcga ttccgcaaaa aaa
//
LOCUS       DASRBV010000030         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_355917, whole genome shotgun sequence.
ACCESSION   DASRBV010000030 DASRBV010000000
VERSION     DASRBV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355917"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VFA02_01305"
     CDS             <1..1542
                     /locus_tag="VFA02_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU08512.1"
                     /translation="HIDDKKSPKDVDDSTDMYDTLEFLLKNVPNNNGKAGIWGISYPG
                     FYTSASMIDSHPALKAASPQAPMTDLFMGDDAYHGGAFMLDANFGFYTFFKPFDGPSV
                     PRPSVPFDMGTPNTYEFYLNAGPTSNLDKKYLKGENWLFADQLYHTTYDDYWKARDLS
                     QHMKNVKCAVLVVGGWFDAEDLSGPFKTYYAVSKFNPETPDTLVEGPWVHGGWARNNG
                     DHLGDVQFNSKTSEFFRKNIQFPFFEYYLKGKGSPVAKVYAFETGTNVWHTLDQWPPA
                     QAKPETLYFHANGKLSFDPPSADEGQDEYVSDPAHPVPFVGYVTDTVPQRYMVDDQRF
                     ASTRTDVLVYQTDPLPEDVTIAGPVSPKLKIATTGTDSDFVAKLIDVYPNDYPDPDDE
                     SRTGKRVIDTPAIRMGGYQQLLRGEPMRGKFRHSVEKPEPFAPGKMEEVDFTMPDLYH
                     TFRRGHRIMVQIQSSWFPLVDRNPQTFTDIPNAKPEDFRKATETVFHKANAASGVEVL
                     VMPKP"
     gene            complement(1578..2231)
                     /gene="udk"
                     /locus_tag="VFA02_01310"
     CDS             complement(1578..2231)
                     /gene="udk"
                     /locus_tag="VFA02_01310"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HZU08513.1"
                     /translation="MNGGPVIIGIAGCSGSGKTTLAQELARELEGTHFHLDHYYRDLG
                     HLTYAERCQQNFDHPDALESDLLIAHIRRLASGRSIHQPQYDFATHTRIPGKTVLLYE
                     PHFLLVDGILALHYPALRRLYHLRVYVDTPDEVCYQRRFARDVEQRGRTPESVAEHYA
                     ATVRPMAEKFVRPSLQYADLVVDGTASFDWSVEQVLGKLRQMKLLSAFELNSLAGHS"
     gene            complement(2228..3073)
                     /locus_tag="VFA02_01315"
     CDS             complement(2228..3073)
                     /locus_tag="VFA02_01315"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZU08514.1"
                     /translation="MLSQFEKVSAAADYIRAKIRIVPSVGIILGSGLGPFAEKIPDAV
                     AVPYSEVPHFPQSTVPGHSGRLVAGRLDAVPVIAMQGRVHGYEGYTPEEVAFPVRVLG
                     FLGVKTLVVTNAAGGIRPDMKQGDLVLISDHINLTQRNPIAGENDERFGTRFFDMSEA
                     YSRRLRKLAADIARGEGISLEEGVYLCVLGPSFETPAEIRAFRALGADLVGMSTVQEV
                     IAARQMGIEVLGISCVTNMAAGIQCEPLSHEEVIETGQRAAQKFTDLLTALVPALQQL
                     ETAKA"
     gene            3186..3635
                     /locus_tag="VFA02_01320"
     CDS             3186..3635
                     /locus_tag="VFA02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HZU08515.1"
                     /translation="MQHQTATQTLTYPATMYAPPALPDLSRADERRRLSPSALRAFFN
                     IMQKWQVRDEDARQLLGGVSNGTFYQMKQNPNRVLDQDRLLRISYLIGIFKALNILYS
                     ARLADQWVRLQNTNPIFAGRTPLEYMLQGGTPAMQVVRRLLDARRGG"
     gene            complement(3900..4676)
                     /locus_tag="VFA02_01325"
     CDS             complement(3900..4676)
                     /locus_tag="VFA02_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU08516.1"
                     /translation="MSEFLRMEHVNVARGEAVVLHDINLSIQTGEHVAILGPNGCGKS
                     TLIKTITCECYPIFTPEMHCRLLGRERWDVSQLRQHLGVVSADMPGERTPVSKGRDVV
                     VAGFFSASTIWPNLHITKEMRERAQEAMALMEATHLADKLVGEMSAGEQRRIMIARAL
                     VHKPEMLLLDEPSNALDLAAQRELRETLRVVAQQGTGILMVTHHLADILPEIDRVIMM
                     RSGSIVADGRKAELITAERLRGLFDVEVTLTQRDGYWHSW"
     gene            4727..5947
                     /gene="argJ"
                     /locus_tag="VFA02_01330"
     CDS             4727..5947
                     /gene="argJ"
                     /locus_tag="VFA02_01330"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZU08517.1"
                     /translation="MNDSSLLSDDALPLGFRFAAVKAGIKPSGNLDFAVALADAPASA
                     AAMFTSNRVVAAPIIVGRKHLERSKGRVRAVVVNAGNANCATGQAGIDACLQVCAETA
                     KTFACEGHEVFPSSTGIIGVPLPAEKPITALPQIEASLAATPEQFSLFARAIMTTDTK
                     PKVAHAQVQVNGKKVRIAGVSKGAGMIHPQLVPHATMLVYLFTDADVQPQDLRSLLQK
                     SVESSFNRISIDGDTSTNDTVLLLASGASGAKVSASDAAFSTTLTEVCTSLAKQIVDD
                     GEGVTHVVELQIEGASTDRDALAIAKAIAHSPLVKTAWAGCDPNWGRLMAAAGYSGAA
                     IDPERISIWFGNLPICRNGGRAPEFDEASAHEYLKQREFTIRLDFGLGSGSCRFWTTD
                     LTVEYVHINADYST"
BASE COUNT         1490 a         1857 c         1590 g         1306 t
ORIGIN      
        1 cacattgacg ataaaaagtc tccaaaagac gtagacgaca gtacagatat gtacgacacg
       61 ctcgagttcc tgctgaagaa cgttcccaat aacaacggca aggcgggaat ctggggcata
      121 tcctacccgg gcttttacac ctcggccagc atgattgatt cgcatccggc gctgaaggcc
      181 gcatcgccgc aggccccaat gactgacctc tttatgggcg atgatgcata tcatggcggc
      241 gctttcatgc tcgacgctaa ctttggtttc tacacttttt tcaaaccgtt cgatgggcca
      301 tcggttccaa ggccttcggt tccatttgat atgggcactc ccaacacata tgagttctat
      361 ctgaatgcgg ggcctacctc aaacctcgac aagaaatatc tgaagggcga aaactggctc
      421 tttgccgacc agctgtatca caccacatac gacgattatt ggaaggctcg cgacctctca
      481 cagcatatga agaacgtgaa atgtgctgtg cttgtcgttg gcggatggtt cgatgcggaa
      541 gacctctccg gaccattcaa aacctattac gccgtcagca aattcaatcc tgaaacaccg
      601 gacacgctcg tcgaaggccc gtgggtgcat ggcggatggg cgcgtaataa cggcgatcat
      661 ctcggcgacg tgcagttcaa ctccaagact tcagagtttt tccgcaaaaa cattcagttc
      721 cctttcttcg agtattacct aaagggaaaa ggctctccgg ttgctaaagt ttatgctttt
      781 gaaaccggca caaacgtctg gcacacactg gaccaatggc cacccgcaca ggcaaagccg
      841 gagacgctct actttcatgc aaatggaaag ctctcatttg acccgccgtc cgcagacgaa
      901 ggtcaggatg aatacgtgag cgacccggcc catccggtgc cttttgtcgg ctatgtgact
      961 gacaccgtcc cccagcgcta tatggtagac gatcagcggt tcgcctccac ccgcaccgat
     1021 gttctcgtct accagacaga ccccctccca gaagatgtga ccatcgccgg cccggtttcg
     1081 ccaaagctca aaattgcaac caccggtacc gattcagact ttgtcgcaaa gctgattgat
     1141 gtctatccaa atgactaccc tgatccggat gatgaaagca gaactggcaa gcgcgtgatt
     1201 gatacgccgg ccatccgcat gggcggatac cagcagctcc tgcgcggtga gccgatgcgc
     1261 ggcaaattcc gccatagcgt tgaaaaaccc gaaccatttg ctccgggcaa aatggaagag
     1321 gtggatttca ccatgcccga cctctaccac acgttccgtc gcggacaccg cattatggtg
     1381 cagatacaaa gctcctggtt cccgctggtg gaccgcaatc cgcagacgtt caccgacatt
     1441 cccaacgcca aaccagaaga cttcagaaag gccaccgaga cggtctttca caaagcaaat
     1501 gctgcttctg gagtggaggt cctggtgatg ccaaagcctt agtcagcagg tagaaagaga
     1561 agccaaaaat tctgaattta tgaatgtccg gcgagactgt tcagttcgaa ggcactgagc
     1621 agcttcatct ggcgcagctt tccgagcacc tgctcaaccg accagtcgaa agaagcggtc
     1681 ccgtctacaa ccagatctgc atattgcaat gaaggacgga cgaacttctc agccataggc
     1741 cgcacagtgg ccgcataatg ttcagcaaca gattcaggcg tccttcctct ctgctctaca
     1801 tcgcgcgcaa aacggcgctg atagcaaacc tcgtccggag tatctacata tacgcgcaga
     1861 tgatagagcc gccgcaatgc cggatagtgc agcgcaagaa ttccatctac cagcagaaaa
     1921 tgcggctcgt acagcaatac agtctttccg ggaatgcggg tgtgagtagc aaaatcatac
     1981 tgcggctgat gaatgctgcg gccagaagcg agccggcgga tgtgcgcgat cagcaggtct
     2041 gattccagcg cgtcaggatg atcaaaattc tgttgacatc gctcggcata ggtaagatgt
     2101 ccaagatcgc gatagtaatg gtccagatgg aaatgcgtcc cttcaagctc gcgggccagc
     2161 tcctgcgcca gtgtcgtctt ccctgacccg gaacagcctg caataccaat gatcaccggg
     2221 cccccattca tgctttcgct gtctccagtt gctgcaaagc agggaccagc gcggtgagca
     2281 ggtcggtaaa cttctgcgca gcccgctgcc cggtctcgat gacttcttcg tgcgagagcg
     2341 gctcacattg aataccagca gccatgttcg ttacacagga gatgccgagg acctcaatcc
     2401 ccatctgccg tgccgcaatc acctcctgca ccgtagacat gccaaccaga tcagctccca
     2461 gggcgcggaa ggcccgaatt tcagccggcg tctcaaaact tgggcccagc acacagaggt
     2521 acaccccttc ttcgagcgag atgccctctc cccttgcaat atcggcagcc aactttctca
     2581 gccgcctgga atacgcctcg gacatatcga agaaccgtgt gccaaagcgc tcgtcatttt
     2641 cgcccgcgat tgggttccgc tgcgtgagat tgatgtggtc ggaaataaga acaaggtctc
     2701 cctgcttcat atccgggcga atcccgcccg cagcgttggt aacaaccagc gttttcaccc
     2761 ccagaaaacc gagcacccgg accggaaatg ctacttcctc cggtgtatat ccttcatacc
     2821 catgcacccg gccctgcatt gcaatcacag gaacagcatc aagcctgcct gcaacgagcc
     2881 gtccagagtg cccaggtacc gtggattgag gaaaatgagg gacctcgcta tatggaacag
     2941 caaccgcatc cgggatcttt tctgcaaatg ggccaagtcc tgaaccgaga atgattccaa
     3001 cagaaggaac aatgcgaatc ttggcccgaa tgtagtccgc cgcggcggaa accttttcaa
     3061 actgagagag catgtttcct gtagattaca tttcccggtc cccctgaaca aagctgaaac
     3121 cagaaattta ttgcattcag agagttatgg agatatgata ctccataaat ggagccataa
     3181 gaggaatgca gcatcaaacc gccacccaga cccttacgta tccagcgaca atgtacgctc
     3241 ccccggcctt gcctgacctt tcacgtgccg acgagcgccg ccgcctctcc ccttcagcac
     3301 tgcgggcctt cttcaacatc atgcaaaaat ggcaggtgcg cgatgaagat gcgcgccagt
     3361 tgcttggcgg agtttcgaat ggcaccttct accagatgaa gcagaaccca aaccgtgtgc
     3421 ttgaccagga ccgcttgctg cgcatctcgt atctgatcgg aatcttcaaa gcactgaaca
     3481 ttctctatag cgccaggctg gccgaccagt gggtgcggct ccagaatacg aaccccattt
     3541 ttgcaggacg cacaccgctt gagtacatgc tgcagggtgg tactccggca atgcaggtcg
     3601 tgcgccggct gctcgatgca aggcgtggcg gatgaactgc ccaattctct ttcaataaaa
     3661 gcaaaggccc gcagccattt gcgggccttt gccggtgaac tgttgggatt gacctgaggc
     3721 acccttcgtc tcgcatgggc ctcggcggaa atgccaggac tggagcgtag agcgccccta
     3781 agcctcagtc acctcacgat gggtcatgta actactaccg tcagaattct tcattcgctg
     3841 gaccacctcc tttcccgtgt acttgtgacc ttaagctccg ccgcttgaaa aatcaagtgt
     3901 caccaggaat gccaatagcc atcgcgctga gtcagcgtga cctccacgtc aaacagcccg
     3961 cgcagcctct cggcagtgat caactcagct ttgcggccgt ccgccacaat actcccgcta
     4021 cgcatcatga tcacgcggtc tatctccggc agaatgtcag ccagatggtg cgtcaccatg
     4081 aggatgcctg tcccttgctg tgccacaaca cgcagcgttt cgcgtagttc ccgctgcgcg
     4141 gccaggtcca gcgcattcga tggctcatca agtagcaaca tctccggctt atgcacaagg
     4201 gcacgggcaa tcatgatgcg gcgctgctct ccggcggaca tctcgccaac cagtttatcc
     4261 gccagatgcg ttgcttccat cagggccatc gcctcctgcg cgcgctcacg catttctttg
     4321 gtgatatgca gattcggcca gatcgtggac gcagaaaaga accccgccac aacaacatcg
     4381 cggcctttgg agaccggcgt gcgctctccg ggcatatcgg cggaaacaac gccgaggtgc
     4441 tggcgcagtt gggaaacgtc ccagcgctcg cgccccagca gacggcagtg catctctggc
     4501 gtaaagatgg gataacactc gcaggtgatg gtcttaatca gagtggattt gccgcatcca
     4561 ttcgggccga gaatggcaac gtgttccccg gtttgaatgc tgaggttgat gtcatgcagc
     4621 actacagctt cgccacgcgc cacattgaca tgctccatgc gcagaaattc tgacaacact
     4681 cgctcctcga taaaagaatc ctttacagtt agagtaaata ctcactatga acgactcatc
     4741 gcttctttca gacgatgcac tgccgcttgg ctttcgcttt gccgcagtca aggccggaat
     4801 caagcccagc ggcaatctcg attttgccgt ggccctggcc gatgcgcctg ccagtgccgc
     4861 cgccatgttc accagcaatc gcgttgtggc tgcaccaatc attgtgggcc gcaagcatct
     4921 ggagcggagc aaaggacgcg tccgtgcagt cgtggtcaat gcaggaaacg caaactgcgc
     4981 taccggccag gcgggaatag atgcctgtct tcaggtctgc gctgagacag caaaaacctt
     5041 tgcctgcgaa ggacacgagg tcttcccctc ctccacagga atcattggcg ttccgcttcc
     5101 cgcagagaag cccatcaccg ctctgccaca gattgaagca tcgctcgcgg caacaccgga
     5161 acagttttct ctcttcgcgc gcgccattat gaccacagat acaaaaccga aggtggccca
     5221 tgcacaggtg caggtgaatg gaaaaaaagt ccgcatcgca ggcgtatcga aaggcgcggg
     5281 catgattcac ccgcagcttg tgccccacgc gacgatgctg gtgtacctgt tcacagatgc
     5341 agatgtccag ccgcaagacc tgcggtccct gcttcagaaa agcgtcgaga gcagcttcaa
     5401 ccgcatctcg attgatggcg atacctccac aaatgacacc gttcttctgc tcgcatccgg
     5461 cgcaagcggt gcaaaggtgt ctgcatcaga cgcagctttc agcaccaccc tgacggaagt
     5521 ctgtacatcg ctggcaaagc agattgttga cgatggcgaa ggcgtgacgc atgtggtcga
     5581 attacagatt gaaggcgcgt ctactgaccg cgacgcactc gccatcgcga aagccatcgc
     5641 gcattcgccg ctggtaaaaa cagcctgggc aggatgcgat ccgaactggg gaaggctgat
     5701 ggccgccgcc ggttattcag gcgctgccat tgacccggaa cgcatcagca tctggtttgg
     5761 caacctgccc atctgccgca acggcggacg cgcgccggaa tttgacgagg catccgcaca
     5821 cgaatacctg aagcagcgag agttcaccat tcgcttggac ttcggcctcg gcagtggctc
     5881 ctgccgcttc tggacaactg acctgaccgt ggaatatgtg catattaacg ccgactactc
     5941 cacttagaaa agggccttgt tgcggaactg ctgcactgca accaaaaatg tgcagagagc
     6001 tagaccccgc gtcattcctg aattttttga tttttgctcc gggaattgca caggcttttt
     6061 cacaattctg ttgaaaacgc ctctcaaaaa ttgcgcggac atccgcttca gattacgctg
     6121 ctaaaattcc agaaacgatg actcccaaat cgagccctcc aaggacattt tccctgatca
     6181 gcaatgcgaa gttgcgccag atgtatgtca cgatgctcca ataccgcatt ctcgcggcgc
     6241 atg
//
LOCUS       DASRBV010000031         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_305243, whole genome shotgun sequence.
ACCESSION   DASRBV010000031 DASRBV010000000
VERSION     DASRBV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305243"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3518
                     /locus_tag="VFA02_01335"
     CDS             <1..3518
                     /locus_tag="VFA02_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08518.1"
                     /translation="LNICFLLDMVLAFGVCAHGQVIGATISGTIMDQQHMVVPGATIM
                     AVQQQQNVLQATTSNNQGQFHLVQLKPGTYRLRVEKPGFKTYEQAGIVLEGNADITLP
                     AILLRVGSANETVTVLSQGMHLETENNERGFTVNNQQMEDILVNGRSWAALAALVPGV
                     VSNGNYKTASNNGLNSTYVNGNRDISNQVTVDGIGNQDQGNNADQLATVSMDNIQELR
                     IVTDNFQAQYGRNTGSQTTIVTKSGTREYHGEGYIYHRNESMNANNWFNKRNGQPRNP
                     FRFNDAGYNIGGPLQIPGLFTQWKDKAFFYLGQEWQEQLVPEGTHYARVPTDLERNGD
                     FSQSVDKNNNPWPYIRDYTTGQPCNASNTSGCFQDGGVLGRIPQGRLYALGVKAMQTL
                     PEPNFTGGVGFNYISAISGRYPRREDILRVDYNPSERWKIFYRWLGNEDNAYSWYGTN
                     QIDSGSNIPVSLDRYSRPATNMAIGITTIFNATTTNQFIAGYGKNWLHGSSVDNNKTK
                     AKLGLEDFPVLYPSVVQADLVPTFSFNGSRLANNAVMNTGGTNWHNAATDIELSDNFN
                     KLLGTHSTQFGVYFQHSRKDQIQYWYASGNYNFGDDPSNPYDSQYGLANFALGIYDTY
                     QQANSYIYPMFHTATVEWYGQDSWRVTNKWTISYGMRFSWMPPEYEQNNNSAVFDPAA
                     FQPSESVRLYQRRWSGSKWYAYDPVTGQTLPAYAIGDLVPNSGNQLNGIRIAGKAIDK
                     HLMEVRGVQYGPRLGMSYLLTDKTVLRGGGGIFYQRTSSNNEMNYSANVPFQYQETQY
                     YGLVSDLTSNTSLLSPFGTRAREWAAKIPTTYDFNLELQQELPYQMLWTVAYVGSNNS
                     HLYQQMNLNGIPYGATFLPQNQDPAKVAQNPSNRPGSNAYDENFIRPYVGYGDIPLVM
                     TGGSSNYNSLQITVNRRYAHGLFLGGAYTWGKALGVTTNEGDWIRIDGQTRKYNYGPL
                     GFDRRQIFAANYIYELPFLFHTNSLMHAIADGWQVAGITRFQTGGEFTPGASIPGWSN
                     ANVTGSYSEGWRIQLVGNPHAGTSHSPYHRLNPQAFQAPSIGNIGGGMGLNSITGPGI
                     NNTDLSLEKKFHIEERGEIQLRCDAFNVFNHPQFNGINASCTFTAPGQTGCTNPAGPG
                     NLTGFGSVSGANDPRILQLSTRIKF"
     gene            complement(3541..>4724)
                     /locus_tag="VFA02_01340"
     CDS             complement(3541..>4724)
                     /locus_tag="VFA02_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZU08519.1"
                     /translation="WKVALDRTAFYPASGGQPHDLGTLTATSRSGAVLEVPVIAVEED
                     GHGEVWHHTLKPLLAGTEITGEIDWTRRFDHMQQHSGQHLLSAVFLRELNAPTMSFHL
                     GEISSTIDLSLESLAPQSVERVERIANEIIAEDRPITMRTISREEAEALLAAGHLRKL
                     PDRQGNIRLIEIADCDLNACGGTHVRSTGQIGGLLIRGIERVRQGVRVEFVCGLRAVM
                     AARQDFSALTRSAGLLSTPRADVPVAIEKVLGESKASAKAKQKLYEELANYHTARLLV
                     EVWMKDGLRIVRRTFPDRDADYVKLLASRLTSAAPHTVALLASTLREPAFVVVAQSGD
                     LDFHAGQILKEALAKMGLRGGGSATLAQGDAPQGHLHSLLDSLETTVREAGTLVSNDT
                     A"
BASE COUNT         1144 a         1264 c         1306 g         1010 t
ORIGIN      
        1 tcctgaacat ctgttttttg ctggacatgg tgctggcttt tggcgtctgc gcgcatggtc
       61 aggtcatagg cgcgacgatc tccggcacca tcatggacca gcagcacatg gttgtgcccg
      121 gggcaaccat catggcagtg cagcagcagc agaacgtcct tcaggccaca accagtaaca
      181 atcaagggca gttccatctt gtacagctca agcctggaac gtacaggctc agggtggaaa
      241 agccagggtt caagacgtat gaacaggcgg gaattgtgct ggaggggaac gcagatatta
      301 ccctgcctgc cattctgctg cgggtcggtt cggcgaatga aacagtcacg gtgctttccc
      361 aggggatgca tctggaaaca gaaaacaacg aacgcggttt caccgtaaat aaccagcaga
      421 tggaggacat cttggtgaat gggcggagct gggccgcgct ggcggcgctg gttcccggcg
      481 tggtcagcaa tgggaactat aagacggcca gcaacaatgg cctgaattcc acttatgtga
      541 acggaaatcg cgatatcagc aatcaggtga cagtggatgg aatcggcaac caggaccagg
      601 gaaataatgc agaccagctg gccaccgtca gcatggacaa tattcaggaa ctgaggattg
      661 ttacagacaa cttccaggcg cagtatgggc gcaataccgg ttcacaaacc accatcgtta
      721 cgaagagcgg cacccgggaa tatcacggcg aaggctatat ctatcatcgc aacgaaagca
      781 tgaatgccaa caactggttc aataagcgaa atggccagcc cagaaaccct ttccgcttca
      841 atgatgcggg atacaacatc ggcggtccgc ttcagattcc agggctcttc actcaatgga
      901 aagacaaagc attcttttat ctggggcagg aatggcagga acaattggta ccggaaggca
      961 cccattatgc gcgcgtgccc accgacctcg agaggaacgg ggacttttcg caaagcgtgg
     1021 acaagaacaa caatccgtgg ccttacattc gcgattacac gaccgggcag ccctgcaatg
     1081 cttccaatac atcgggttgt tttcaggatg gaggagttct gggaaggatt cctcaaggca
     1141 ggctgtacgc acttggtgtg aaagcgatgc agaccctgcc tgagccaaac ttcacgggag
     1201 gagttggctt taactacatt tctgcgattt ccgggagata tccacggcgt gaagacattc
     1261 tgcgggttga ctataaccca tcggaacggt ggaagatttt ctatcgctgg ctaggcaatg
     1321 aagacaacgc ttatagctgg tatggaacga accagattga cagcggatca aacatcccgg
     1381 tttctcttga ccgctattca cgccctgcga cgaacatggc gattggcatc acgacaattt
     1441 tcaatgcaac tacgacgaac cagttcattg ccggatatgg aaaaaactgg ttgcacggca
     1501 gttcggtaga caacaacaag acgaaggcca aactcgggct ggaggatttt cctgtgttgt
     1561 atccttctgt tgttcaggcc gaccttgttc ctaccttttc tttcaacgga tcgcgtctgg
     1621 ccaataatgc agtgatgaat acgggcggca cgaactggca caatgcagcc accgatattg
     1681 aactcagcga caacttcaac aaactgctcg gcacccactc tacgcagttc ggggtctact
     1741 tccagcattc gcggaaagac cagatccaat actggtatgc atcaggaaac tacaactttg
     1801 gcgacgaccc cagcaatccc tacgacagcc agtacggtct ggcgaacttc gcgctgggaa
     1861 tttatgacac ctatcagcag gcaaactcct atatctatcc tatgttccac acggccaccg
     1921 tggaatggta tgggcaggat tcctggaggg tcacaaacaa atggaccatc agctatggga
     1981 tgcgcttttc ctggatgcct ccggagtatg aacaaaacaa caattccgcc gtgtttgacc
     2041 cggctgcttt tcagccctcg gagtcggtgc gtctttatca gagaaggtgg agcgggtcca
     2101 aatggtatgc ctacgatccg gttacggggc aaacgctgcc ggcgtatgca attggcgatc
     2161 tcgtgccgaa ctcgggcaat caactgaatg gcatccgcat agcaggtaag gccattgata
     2221 agcatctgat ggaagtgcgc ggcgtccagt atggcccgcg cctgggaatg tcatatctgc
     2281 tgacagacaa gacggtgctt cgtggcggag gaggtatttt ttatcagcgc acatccagca
     2341 acaacgagat gaattacagc gccaatgttc ctttccagta ccaggagacg cagtattacg
     2401 gtctggtcag cgatctgacc tccaatacct cgctccttag cccattcgga acgagagcaa
     2461 gggagtgggc ggcgaaaatt cccacgactt acgatttcaa tctggagctc cagcaggaac
     2521 ttccatatca gatgttgtgg accgtcgcat acgtcgggtc aaataacagc catttgtatc
     2581 agcagatgaa cctgaatggc attccttatg gggcgacctt tcttccgcag aaccaggacc
     2641 cggccaaagt agcgcagaat ccaagcaacc gcccaggttc gaatgcatac gatgaaaact
     2701 tcatccgccc atatgttggc tatggagata ttccgctggt catgactggg ggatcgagca
     2761 actacaactc tctccagatc accgtgaacc gccgctatgc gcacggactc tttttaggcg
     2821 gcgcctatac ctgggggaag gccctgggtg taacaacaaa tgaaggagac tggatccgta
     2881 ttgatggcca gacgcgtaaa tacaattacg gcccacttgg atttgacaga aggcaaatct
     2941 tcgcagccaa ttacatctac gagctgccat ttctttttca tacaaattcc cttatgcatg
     3001 cgattgccga tggctggcag gtggctggga tcacgcgctt tcagacagga ggagaattta
     3061 ctcccggcgc gagcatcccg ggctggtcta acgccaatgt caccgggtcc tattctgaag
     3121 gatggcgtat tcagcttgta ggaaatcctc atgcagggac cagtcatagt ccgtatcaca
     3181 ggttgaatcc gcaggcattt caggcgccgt ccattggcaa cattggcggc ggcatgggcc
     3241 tgaacagtat taccggcccc ggcatcaaca acacggacct ttcgctggaa aagaaatttc
     3301 atatagaaga gcgaggtgaa atccagctcc gttgcgatgc tttcaacgtg tttaatcacc
     3361 cgcaattcaa tggcattaat gccagttgca ctttcaccgc tccaggacag acgggatgca
     3421 caaacccagc cggtccgggg aatctgacgg gattcggttc agtgtcgggg gccaatgatc
     3481 cgcgcattct gcaattaagc acgcgaatca agttctagct gtcaagccgg ctgctgggct
     3541 tcaggctgtg tcgttggaaa cgagtgtgcc tgcttcgcgg acggtcgttt ccagtgagtc
     3601 cagcagcgaa tgcaggtggc cctgcggtgc atctccctgg gcgagagtgg cggagccgcc
     3661 cccgcggaga cccatttttg ccagtgcttc tttcaagatc tgaccggcat ggaaatcgag
     3721 atcgccgctc tgtgccacaa caacaaaggc tggttcgcgc agtgtagaag ccaataaggc
     3781 cacggtgtgg ggagctgccg aggtcaaccg tgaagccagc agcttgacat agtctgcatc
     3841 gcggtccggg aaggtccggc gcacgatgcg caagccatct ttcatccaca cttctaccag
     3901 cagacgggcc gtgtgataat tggccagctc ttcataaagt ttctgttttg ccttggcgga
     3961 cgctttcgac tcgcccagga ctttctctat cgccacggga acatcagcgc gcggagttga
     4021 aagcagtcct gcactgcgtg tgagcgcgga aaaatcctgc cgcgcagcca tcactgcgcg
     4081 caatccgcag acaaattcaa cgcgcacgcc ctggcggacg cgctcaatgc cacgaataag
     4141 cagcccacca atctgtcctg tcgaacggac atgcgtgccg ccgcaggcgt tgaggtcgca
     4201 gtccgcaatt tcaatcagcc ggatgtttcc ctggcggtca ggaagtttgc gcagatgtcc
     4261 ggcggcgagc aaagcttctg cttcttcgcg gctgatagtg cgcattgtga tcgggcggtc
     4321 ttctgcaatg atttcgttgg caatgcgttc gacccgctcg acgctttgcg gagcgagcga
     4381 ctccaggctg aggtcaattg tggaagagat ttctccaaga tggaaggaca tggtaggcgc
     4441 attcaattcg cgcaaaaaaa ccgcggagag aaggtgctgg cccgaatgct gctgcatgtg
     4501 gtcgaagcgc cgcgtccagt caatttcgcc agtgatttcg gtgccggcca gaaggggctt
     4561 cagcgtatgg tgccaaacct cgccgtgtcc gtcttcttcc accgcgatga caggcacctc
     4621 cagtaccgcc ccgctgcgcg aagtggccgt aagcgttccg agatcatggg gctgacctcc
     4681 gctggccggg taaaacgccg tgcggtcgag ggcgaccttc caga
//
LOCUS       DASRBV010000032        26426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_661081, whole genome shotgun sequence.
ACCESSION   DASRBV010000032 DASRBV010000000
VERSION     DASRBV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661081"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFA02_01345"
     CDS             complement(<1..717)
                     /locus_tag="VFA02_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU08520.1"
                     /translation="MTTTEFSAWIFLSSVLAGLLGALTGLGGGVVVVPVLTLLFHVDI
                     RYAIGASLVSVIATSSGAAAAYVREGFSNVRIGMFLEIATTLGALLGAYLTAIVSTHA
                     IGIIFGLVLLYSAWASFRAHPDTVERNPDTLALKLKLQGTYPGPSGEEAYVAQRVPAG
                     FSLMFLAGTLSGLLGIGSGAVKVLAMDQAMRLPFKVSTTTSNFMIGVTAAASAGIYLS
                     RGYISPGLAMPVMLGVLLGSL"
     gene            complement(724..2466)
                     /gene="treZ"
                     /locus_tag="VFA02_01350"
     CDS             complement(724..2466)
                     /gene="treZ"
                     /locus_tag="VFA02_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZU08521.1"
                     /translation="MHRFRVWAPKANTVDVKIAGQLHPMHKTTGGWWETNVPSASAGT
                     DYAFIVDRQTPDLPDPRSPWQPHGVHGLSRILDHAAFHWTDEHWQQPPLSSAILYEMH
                     VGTFTPEGTFDAARERLPYLRDLGITHVELMPVASFPGTRGWGYDGVDLFAPQEAYGG
                     PEALKRFVNACHEHELAVVLDVVYNHLGPSGNYLGKFGPYFTHLHNTPWGDAVNLEDG
                     GSHEVRRFFCDNALMWLRDYHFDGLRLDAVHAYMDRSAIHFMEQLAGEVRVLEAQTGR
                     QYVVIAESDLNDPRLVRAPEAGGYGMDAQWSDDFHHAIFALLTGDRNGYYADFGSMAD
                     LAKSLKSVFVYDGIYSAYRDRIQGRPLDGLPAWRFLGYSQNHDQVGNRAKGDRLCHIT
                     SIERAKIAAAIVFTAPFVPMIFQGEEWAASSPFQYFTDHEPELGRLVSEGRRREFAAF
                     GWKPEEVPDPQDEQTFLRSKLHWDELAKPPHSAMLEWYRQLISLRRSHPDLTEGSLEH
                     LHIEFSEEGRWLVMQRGSVEVLVNLGQRALVRQTSSTLLLLSSGTFVKLEDGIVSLSP
                     DSVAILKREQQVSC"
     gene            2592..3104
                     /locus_tag="VFA02_01355"
     CDS             2592..3104
                     /locus_tag="VFA02_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU08522.1"
                     /translation="MKLGEKIRYLREVEGNLRGLNRAMTQQEVVKAIRSELKAQGEAK
                     ISQSYLSQIESGARPHLTNTTRQLLAKFFKVHPGYLVDDPEGYHPELLSDARTLEDKL
                     DLWLVSGAERFRRDPALRQALLTLARHKETRRCLLLLEEIIETPGLVERLLEVLRPAA
                     KRRSVKENQK"
     gene            3101..3679
                     /locus_tag="VFA02_01360"
     CDS             3101..3679
                     /locus_tag="VFA02_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08523.1"
                     /translation="MTWEDFYLFCFAFGLCLSAIAFVGGFSHFHLGHLHVHHAGGAGH
                     AHGGSGQSINPINGFTLMIFLCWFGGTGYLLERYGGFVVPVVLLLSTLAGIAGAGAIF
                     WFLVRVLLPHERMLTAEETATAGMVARVSAPIRDHGTGEILFTQNGSRKFAAARSENG
                     LPIPRDTEVVVMRYEQGIAWVRRWDEVAGDMK"
     gene            3746..5170
                     /locus_tag="VFA02_01365"
     CDS             3746..5170
                     /locus_tag="VFA02_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZU08524.1"
                     /translation="MTNTIIVIVGLSLLALIILVGLLAKMFRKAGPNEALIVYGFRGP
                     RVIKGHGTVIFPMVESCRELSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVRSDQE
                     SILTAAEQFLTKTPPQREGLIRLVMEGHLRGIIGQLTVEQIVKEPEMVSDRMRSTCAD
                     DMSKMGLEVVSFTIKEVRDKNEYITNMGRPDIARIKRDADVAAAEAERDTAIRRANAQ
                     REAAIAKAAADQERVIAETASLAKQAEAQRDLDIRKAQFAEQSRKQQAQADKAYELQT
                     NVMQQQVVAEQVKVQQIEKEQQIKVQEAEIQRHEKELIATVLKQAEIERQRIENLAAA
                     EKSRLMIEAEGQAAAIRAQGEAEAAVIFQKGEAEAKAMNVKAEAYQEWNQAAVVDRLI
                     SNMADVVRAMSEPLSKVDKITVVSTGSDGSAGVNKLTGDIAKIAAQVPALFEALSGMD
                     MRELMNNVKTMRARPNGGPTVEGK"
     gene            5224..5892
                     /locus_tag="VFA02_01370"
     CDS             5224..5892
                     /locus_tag="VFA02_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZU08525.1"
                     /translation="MALLERVAALLRANVNDLMDRAEDPEKMMKQLVLDMENQLLQVK
                     TQVAIAIADQHLLEKKKKEHDDEAAEWHKKAELAVARGKDDLARAALERALGHEQMAS
                     GFAQQIEDQSAEAESLRSALRKLEQKLAETRSRCEMLIAQHRRARVVNRANQVRQKMD
                     ATSNSASIDRMKLRVIGTESENAAVHDLLKEDALEDRFAALEREDRIEQLLRDLKERQ
                     GKTA"
     gene            5936..7402
                     /locus_tag="VFA02_01375"
     CDS             5936..7402
                     /locus_tag="VFA02_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZU08526.1"
                     /translation="MDHSNGFDLQAAARQSMLDHGFEAGFSQAVKDEVVAIQEHPLSV
                     DGFDGVRDMRELPWSSIDNDTSRDLDQIEAIAEMNDGAVRVRVGIADVDAYVPKGSAI
                     DRFAGKQTTTVYTGVHNFSMLPEELSTGLSSLLENDDRLSVVYEFVVDAQGCVNSSAI
                     YRATVRNKAQLAYPSVGAWLETGANPPEKVAASEEIKQQLQIQNCVAQLLRSQRYKRG
                     ALNLQTIETNPLMRDGAPVEIQVHMRNPATDLIEDFMIAANEVVATSLEKAKLSSIRR
                     VVKTPKRWDRIVELAAQYGTKLPDTPDPKPLHDFLCERQKADPDHFPDLSLAVVKLLG
                     SGEYVLERAGETAEGHFGLAVQDYTHSTAPNRRYADLVTQRLVKAMLAKQKPPYSDDE
                     LAAIAQQCTRQEDAARKVEREMRKRIAAVVMHDRIGQQFAAIVTGVNEHGTFVRTKAP
                     HVEGMLVRGAKGVDVGDKIQVTLIHTDPGRGYIDFARS"
     gene            7412..9967
                     /locus_tag="VFA02_01380"
     CDS             7412..9967
                     /locus_tag="VFA02_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU08527.1"
                     /translation="MRPHLGTLVDDFERHGKEIAIVTYRGNRRIVATYGEVAGLARRF
                     AALLRQQDIVAGERVLIWGQNSAEWVAAFFGCVLRGVMAVPLDAACTPEFAKRVFDDT
                     QPKLVAGDPGLLSQLPEGPAKVILDDLKTSLPPEPQSRITEPSLSLDTPLQVLFTSGT
                     TSEPKGVVHTHRNVLASITPIEREMQKYLKYERPFHPLRFLHTLPLSHVFGQFMGLWL
                     PVLLAAEVHFESRLQAQRLTEMMQRERISVLAAVPRVLDLLRAHLLALFPQLPEQITR
                     AQGEPVWKRWWRFRKVHRLLGWKFWAFVCGGASLPVELECFWNTLGFALVQGYGMTET
                     TALITLNHPFKVGKGTIGKPLPGRDVRISEEGEILVRGDMVSTATWQRGQLIRSADEW
                     LATGDLARSDEEGRFQFLGRKGQMIVTSSGLNIHPEDVEAVLDKQPNVRASVVVPAKM
                     PGGTEAMAVLLFRGAEPEATETIRAANRQLAEYQRIRHWRLWPGLDFPRTSTGKIQRG
                     KVAQWANSVSGEKASPQQNGRDSLLDLIATITHSPAGKTTDDARLDEDLHLDSLGRVQ
                     LQGEIEQKLGITLDDASMIGMETLGELRRALGMNGEDHPGALPESSGAHPQHRPAKYI
                     YPRWPWSWPVQTLRFIFLECVARPLTRFLAKPRIACAPEFAVPQRPVLIIANHVTAYD
                     AALVLYALPGRMRRHVAIAMAADILEDFRHARGQGTWIQNVLAPIAYWLITALFNVFP
                     LPRSAGFRESFAHMGHALDRGYNVLIFPEGTRTSGQLQKFRSGIGMLVQESNAAVLPV
                     ALRGLGELKQAGKGWFRSGRLEVRTGKPIQFQPTDSPEGITATLENTLCSMLE"
     gene            complement(9986..11791)
                     /locus_tag="VFA02_01385"
     CDS             complement(9986..11791)
                     /locus_tag="VFA02_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08528.1"
                     /translation="MKALWLFLCCLLFAAPAIAQDNSEASSSAKTTIVVVGNPAPVTL
                     GESNRSVVIMDTQEHPLAFQLPEDRLRTDPSTFVEQRGAGGAQADIVMRGSSFSQTLV
                     LINGLRVNDAQSAHHNLDLPVPMDAMQSIEILHGSGSTLYGSDAIGGVVDFITASPET
                     TALRLRSGVGSFGENDQAFTGSFTAKRWSELASGARNFSTGFIADRDYRNENGSSETR
                     WKTLLGESDVLLAGSDRAFGADQFYGNFNSWERTKGWFASMRQEIGAKTTAAFGYRRH
                     SDIFVLLRNDPAFYKNQHIDQSWQGALRRSDHVGGRSSLFYGLEGDGDSIHSNNLGNH
                     ARNWGAGYLDADLQWKRASITAGLRQEIISGGHYVTSPTLAGSYWLNESLKLRSAFGY
                     GFRLPSYTDLYYSDPATVGNPGLKPESAWNYEAGLDWFAGAQSYLSITGFYSRQHDAI
                     DYVRANAFDKWHASNLTGLRFAGVESAWTWQAAHNQRVRLAWTLLSGAQQALHGLESE
                     YVFNYPVNNAFAEWTIAPLHGYMLHNRVGITQRYQKDPYPVWDAAIAREKGHIHPYLQ
                     MSNLSNTGYEEIEDVRMPGRSFTGGIDVVIGRMAK"
     gene            11886..12341
                     /locus_tag="VFA02_01390"
     CDS             11886..12341
                     /locus_tag="VFA02_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU08529.1"
                     /translation="MRIETFLEQSPLFEIYRVSRKLEAQLRQILGPNEISFFEALVLV
                     SILFEEPHAVRPSHLAETFSTGRGNISNCISSLEARGLLKRRIDPEDGRSIQLLLKPL
                     GRKCAMEAVRIFDQIQKQFEKKIGIMQLKAALSAVRDVEHLCEEAVSWI"
     gene            12356..13963
                     /locus_tag="VFA02_01395"
     CDS             12356..13963
                     /locus_tag="VFA02_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZU08530.1"
                     /translation="MTINIHHLELLMLLLLLFVVLLAVLARRIGQPYPIIFVLGGLVL
                     SFLSPVPDIALRPDFVFLVVLPPLVFAAAHNTSWRDFRENIISILMLAFGLVAFTVVG
                     VGFAGHYFFPQLTWKTGLVLGAVVSTTDTLAATSIARKLGIPARIVDILEGESLINDA
                     SGLLAVSFTVGIITSGHAPSFFSAVGQLLLLSSGGVVIGLVIAWVHSRLIYRIEAAQI
                     EMTMTLMTPYVAYLAAERLHTSGVMATVACGLYLGHHRARLLSLGARLESPAVWNTVE
                     FILNGLVFLLLGLQISSVLAGIEHLGTGSLIAGGVMVGLIVIALRFVWVFPGARIAQR
                     LRRRFGKEAIVENRQVFLVAWGGMRGVISLAAALSLPESLPDGTPFPARNIIIFFTFC
                     VILITLVGQGLTMPFIIRKLGLAQSGNLPNLEEQMALRRMIQNAISYLQQQKEKDSGS
                     KDVYEAVERAYKRRMALLKNPEEGAKEEVSSQAHRYLEIARAARNVERHTAGELLATN
                     QINDLVYQQLIFDIDLQDARFSAPFAS"
     gene            complement(13985..17377)
                     /locus_tag="VFA02_01400"
     CDS             complement(13985..17377)
                     /locus_tag="VFA02_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08531.1"
                     /translation="MTRKIFLFVFCFCICAVAARAQVNYAVVRGSVYDPQHHAIPGAT
                     VEITAIDTGARREVTSNDSGLYEAAGLLPGEYKVTVTHSGFAQAVQRLQLEVGQEATL
                     DVQLQVGGGTETVTATAIPETLKTTDTSVGEVVDRRSVQQLPLNGRMLIDLMLTVPGA
                     HVSHGAQTGDMNPLYWRPGQRSAVSIGGTRPNANYFLLDGATNTDPTFNTQNLSASPD
                     VVQEFQIEIGSYSADMGGAGGGQVNVVTRSGSSAFHGTAYEYLRNGAMDAHSFNDMGG
                     NKHLVQNNFGAAFGGPLLGKSRRTFFFVNYEALRHIAAMTMTDTVPTEDEVGGDFSMS
                     GVNIYDPATTKPNPNYNPNLPVSASNPQFTREQFQCNGMMNVICPDRISPVAKIMLTK
                     YAPRPNIMMGMNMGGMTMMGQPTVVGAGNDANNYLDVRNEVHYTDQGTLRFDHDFASG
                     DTSFIRYSAGGEHGFMPENLPGFGYLHDNLSQQGVLGWNHIFTPQMLNMATLAISRLS
                     MDHTTENANKNDIVSELGIQGTGYGGPGAWGAPYFNVQGYSPLGDSYSATPMHAWDTV
                     IEGRDTLNWQRGRHSMKFGGAYQKFIWPMWGFFQNRGYYQFTNGFTTDIGANDGTGSA
                     LASFLLGLPVARQGQAGIPQMNLRQWYANGYAQDTWRMTSTTTLNYGLRYEYMSALVD
                     IRYTNSNLVFNSDGVPSVFVGGQNGYPKGLMYPPKADFAPRLGIAQSVPHMGLVMHAA
                     YGIFYTPVDMNTWCNQRHNVPYVFPETAQSDNFTPSIQGFDFPQPVLGKVTVTFTGMP
                     LHPAPQYIQQWSASVEQALGKETTLEIGYLGSGGFHLQRSHLINNAQPGPGPIQPRRP
                     FPKISFVPNTVFPANTVVASTTFPVSTMNYLENTAQSWYDAGYVNLRRRYARGLSLLA
                     NYTFSKSLTNAPDFRSPMFEAAVPQNNYDLKAEKGPACDVRHRFALSAVYDLPAWTRD
                     RFTRGITQNWRASTVFQVQTGFPLTISVFGDTANAGTVLGENPIRANVTGKPVFGPGT
                     RNARTWFNPAAFKAPPAYTFGNAGRNSVYGPGMQTMDLAVVRTFSLTEALKFETRGEF
                     FNALNHTNLGTPDRFVNTAGFGSITEVTTPGREIQVSARLSF"
     gene            complement(17518..17934)
                     /locus_tag="VFA02_01405"
     CDS             complement(17518..17934)
                     /locus_tag="VFA02_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HZU08532.1"
                     /translation="MEPSGTAKVKRLYPVVAVEINPRYGWAVQNAPHPPPDCLKPIWE
                     EYQSLLARPELRIRKEVRQQLLQLIKKHSHTVLPQRKLAIARDPDDNIFLECADAARA
                     DYLMTGNQKHSPKFWKSTKVVTPREFISIAAPHLMG"
     regulatory      17899..18100
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            18188..20557
                     /locus_tag="VFA02_01410"
     CDS             18188..20557
                     /locus_tag="VFA02_01410"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZU08533.1"
                     /translation="MPELQTTLADIAPDFPQPEPSSQMQVRKRNGSLEPVDVNKIVRA
                     VQRCCHGLTHVDPMRVASRTIGGLYNGATTRELDSISIQTAASLIAEEPEYSKLAARL
                     LLSTIVKEVSGQNIYSFSQSIELGHQESLIGRATYGFVVENARKLNHAVDEDLSDRFE
                     YFGLRTVYDRYLLRHPITRRVIETPQYFFMRVSCGLALTVSEAIDFYRLMATHDYMPS
                     SPTLFNSGTRHAQMSSCYLIDSPKDSLEAIYDTYKQVAMLSKFSGGIGLAFHRVRSEG
                     SLIRATNGISNGIVPWLHTLDASVAAVNQGGKRKGACCVYLEPWHADIEQFLELRDNT
                     GDHARRTYNLNLANWIPDIFMRRVEADGTWSLFDPKEVPHFPDLYGEAFDRAYEEAEA
                     QKLYVKQIKARDLYARMVRTLAETGNGWMTFKDTTNLKCNQTGRPENVVHLSNLCTEI
                     TEVTSSGETAVCNLGSINLARHITHGSFDFEKLARTVRTAIPMLDRVIDINYYPVEKA
                     ASANSRWRPVGLGIMGLQDVFFQLGLPFDSSEASVLSTQIQEEIYFHALSASCDLAEK
                     HGPHPAFHETRAAQGKLQFDLWGVVPKDPARWDALKDRIRQNGLRNSLLIAIAPTATI
                     ASIVGCYECIEPQVSNLFKRETLSGEFLQVNRYLVEELKKLGLWTEEMRVRIKLAEGS
                     VQGIEELPEHIRKIYRTVWELPMRAILDMAAERAPWIDQSQSLNLFAESPNIGRLSSM
                     YMYAWKSGIKTTYYLRSRPATRIAKTTVQQSASAVACSLENPGSCEACQ"
     gene            20628..21665
                     /locus_tag="VFA02_01415"
     CDS             20628..21665
                     /locus_tag="VFA02_01415"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZU08534.1"
                     /translation="MSIVTESSVKVPDSILDPGLCLTLRPMRYPIFFEMFKDGIRNTW
                     TVEEVDFSTDLTDLRSKLTPAERHLIQRLVAFFATGDSIVSNNLVLNLYKHINSPEAR
                     LYLSRQLFEEAVHVQFYLTLLDNYIPDPAARAEAFAAVENIPSIARKAQFCMKWMDSI
                     QQLDTLQTEEHRRQFLLNLICFAGCIEGLFFFAAFAYVYFLRSKGLLHGLATGTNWVF
                     RDESCHLEFAFEVVKTVREQHPHLFDRAMEEQVIAMLKEAVDCEAQFAEDLLSGGVAG
                     LSLKDMREYLQYVADSRLQRLGIRPVFGSKNPFPFMDLQDVQELTNFFERRVSAYQVA
                     VQGEVAFHDDF"
     gene            21940..23088
                     /locus_tag="VFA02_01420"
     CDS             21940..23088
                     /locus_tag="VFA02_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HZU08535.1"
                     /translation="MSATVVSNPSAAGTKTRLVSLDVLRGITIAFMILVNNGNQNSYW
                     PLHHIDWNGWTPTDLVFPTFLFVAGVSLVFSFESRLSKGQSKASIFAHAVRRSAILFL
                     LGLVVNGYPYFHLGTLRIYGVLQRFAVCLLMAAVLELWDRGAKSKIAVLAACLIAYWI
                     IMRWVPVPGYGLPGRDIPFLDKDANWVAYLDRHIFPGRLYEGTRDPEGLLSNLPALGT
                     AILGMLTAIWLRSERTIRAKCLGLFAAAIACLLLGKIWGHWFPINKKLWTSSYVLFAG
                     GWSLLGLAICYWLIEIKKWTRGWTFPWIVFGSNAIAAYVFSELLAPTLWTFKFGAAHR
                     SIGQMIYPSVFAKVPDAAFGALLYSLAFVAVCFLPVLVLYRKKIFIKI"
     gene            23186..23701
                     /locus_tag="VFA02_01425"
     CDS             23186..23701
                     /locus_tag="VFA02_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08536.1"
                     /translation="MQEATARKNTEIVQTDSPDEAPQAEVVLEKPRGRFVRGVGKLKP
                     LLPYLTGGLRMIDHGAAQIVAQLLSLAANEGPASAEAPSEIQQELAEIEASHRDLRLS
                     VQDHSLELKRFEDQIVLLRQAVEHNAAEHAEVVEEVDSLRKLILGVGIGLGVLLIVLI
                     TFVGLLLIRHP"
     gene            24029..24808
                     /locus_tag="VFA02_01430"
     CDS             24029..24808
                     /locus_tag="VFA02_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZU08537.1"
                     /translation="MAWYAYCITEKQAFPELLRHRKPIPFESICGIAGNQVFLYPASD
                     LAVIVSEHSPDDTQHERAALEHARVIADCFKTSTVLPFRFGTVFSDDESLRRSIRSNQ
                     RQFLTNIERLRGKAEMHLKVMLDDCCREQIRAYPYSNGHSGTGREYLAQLRENATLQR
                     ERQTRARAVSVQMHRMFSPLAEEITCRRTDSGKMLLDIAHLIEHKSVERYQNKYSSAL
                     QLMKDCQMQLSGPWPPYHFVHRLTTRHHHNGHHATAHAVQA"
     gene            complement(24999..>26426)
                     /locus_tag="VFA02_01435"
     CDS             complement(24999..>26426)
                     /locus_tag="VFA02_01435"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZU08538.1"
                     /translation="SLKEISSQRAEQFLNTFYFQYGHRSIADLAHIAFAIERLSLLAA
                     ISLVDEQRWDGQERSTRYQNFKKSGWFLPEFGQNLAEKEQFSQQIESLFSAYQAISEG
                     MFEHLKTQVPRPAEMKPESYERTLRARAFDVARYLLPLATNTSLGQIVNARTLETQIS
                     RLLSSPYAEIRQLGERLRTAAAGAAWNVHGEELTALQQEIAAVNPALGEKAANLLTRE
                     VKTAPTLVKYAAANRYEIETRRELAQAARELMKDAAIAPAPVVDLVERGESLEVEIAT
                     TLLYRVSHYSYRQLRDHVAALDEAKCNELITLGMRHRGRHDELLREFAAGQALRFDIL
                     MDIGGFRDMHRHRRCVQIIQGLTTAHGYDVPEGIAEAGLLPQYNSAMDAAFTTHAQLT
                     KSAAQAADAADYVLPLGTRIRALFKMDFAEAVYISELRSQPQGHFSYRRVAWEMYEAV
                     KKQHPALAEYFRTNDVNEPVDLLKR"
BASE COUNT         5867 a         7043 c         7427 g         6089 t
ORIGIN      
        1 cagcgaaccc agcagcacgc ccagcatgac aggcatggcc agtcccggag aaatgtaacc
       61 gcgactgaga taaattcccg cgcttgctgc tgccgttaca ccgatcatga aattgcttgt
      121 cgtagttgag actttgaagg gaagccgcat tgcctggtcc atggccagca ctttgactgc
      181 gccggagccg atgcccagca gtccggacag tgttccggcc agaaacatca gactgaagcc
      241 agccggtaca cgctgggcca cgtaggcttc ttctccagat ggtccgggat aggtgccctg
      301 caatttcaac tttaaggcca gtgtgtccgg attccgctcg actgtatcag gatgggcgcg
      361 aaacgatgcc caggccgaat acagcagcac cagtccaaag atgatcccaa tggcatgagt
      421 ggagacaatg gctgttaaat atgcgccgag caatgcgccc agggtcgtcg caatctcaag
      481 aaacatgccg atgcgcacat tggagaaccc ctcgcgcacg taggccgcag ccgctccgga
      541 tgaggttgca atgacagaaa ccagcgacgc tccgatggcg tagcggatgt ctacgtgaaa
      601 gagcagcgtc agcacaggca ctacaacgac ccctccgccg aggccggtga gcgcaccgag
      661 cagtccggcc agaacggagc tgagaaatat ccatgcagag aattcagttg tagtcataat
      721 gacctaacag gatacctgtt gttctctctt gaggatggcg actgagtccg gagagagaga
      781 aacaatcccg tcttccagct ttacgaatgt tccggaagac agcagaagta gtgtggaaga
      841 ggtctgccgt acaagggccc gctgtcccag attgacaagg acctcgacgc tgccgcgctg
      901 catgacaagc catcggcctt cttcgctgaa ctcaatatgc aggtgctcga gcgatccttc
      961 cgtgagatca ggatgggacc tgcggagcga aatgagctgc ctgtaccact ccaacatcgc
     1021 agagtgtggt ggtttggcca gttcgtccca gtgcaacttt gagcggagaa atgtctgctc
     1081 gtcctgcggg tcgggaacct cctcgggctt ccagccaaag gcggcaaact cgcgccggcg
     1141 tccttcggaa acaaggcggc ccaattccgg ttcgtgatca gtgaagtact ggaacggcga
     1201 agaagcggcc cattcctcgc cttgaaaaat catgggcaca aacggtgcag tgaagacaat
     1261 ggcagcggcg attttggcac gttcgatgct ggtgatatgg cagagccggt cacctttggc
     1321 gcgatttcct acctgatcgt ggttttgcga atatcccagg aaccgccagg cgggcaggcc
     1381 atcgagcggg cgcccttgaa tgcggtcacg gtatgcagag taaatgccgt cgtagacgaa
     1441 gacggacttg agcgattttg ccaggtccgc catgctgccg aaatcggcgt agtagccgtt
     1501 gcggtctcct gtaagcagcg caaagatggc atggtgaaaa tcatcgctcc actgcgcgtc
     1561 catgccgtag ccaccagcct ccggcgcgcg gaccagccgt gggtcgttca ggtcgctctc
     1621 ggcaatcacg acgtactgcc tgccagtctg tgcttccagc actcgtacct caccggcaag
     1681 ctgctccatg aaatgaatgg cagagcggtc catataagca tggacggcat cgagccgcag
     1741 gccgtcaaaa tgatagtcgc gcagccacat cagagcgttg tcacagaaaa agcgccggac
     1801 ttcgtggctt ccaccatctt caagattgac ggcatctccc cagggcgtgt tgtgcaaatg
     1861 agtgaaataa ggaccaaact ttccgaggta gtttcctgaa gggccgaggt ggttgtagac
     1921 cacatcgagc accaccgcca gttcatgctc gtggcaggca tttacgaatc gcttcagcgc
     1981 ctccgggccg ccgtatgctt cctgcggagc gaacagatcc acaccgtcgt agccccaccc
     2041 ccgcgtgccg ggaaaggaag cgacaggcat cagctcgacg tgcgtaatgc ccagatcgcg
     2101 cagataaggt agccgttccc gcgcagcatc aaaagtgcct tcaggagtaa aagtgccgac
     2161 gtgcatctcg taaagaatgg cgcttgagag cggtggctgt tgccagtgct cgtctgtcca
     2221 atggaaagca gcgtggtcca ggatgcgcga taggccgtgc accccatgcg gctgccaggg
     2281 tgaacgcgga tcgggaagat cgggcgtctg acggtccacg ataaaagcgt aatcggtccc
     2341 tgctgaagca ctggggacat tggtttccca ccatccaccg gttgttttgt gcatgggatg
     2401 gagctgtccg gcaattttta cgtcaacggt gttcgccttg ggcgcccaga ctctgaatcg
     2461 gtgcatgttt ctcctttacg gtatctgatg caagtggctg ggctggtttg atttgcggac
     2521 ttttccacag cctatttcga tttttcttgc attacattgc aattagcgat ataaatatta
     2581 ctgattgcga tatgaagctt ggtgagaaaa tccgttatct acgcgaagtc gaaggaaacc
     2641 tgcgcggcct gaaccgggcc atgacacagc aggaagttgt gaaggccatc cggtcagaac
     2701 tgaaggccca gggtgaggca aagatcagcc agtcctatct ttcgcagatt gagagcgggg
     2761 cgcgtccgca cctgacgaac accacgcgac aactgctggc gaagttcttc aaggtgcatc
     2821 ccggatacct ggtggatgat ccggagggtt accacccgga attgctgagc gacgcgcgca
     2881 cgctcgaaga caagctggat ttatggcttg taagcggggc cgagcgcttc cgtcgcgatc
     2941 ctgcactacg tcaggcatta ctgacgttgg cgcggcacaa agagacgcgg cgctgtttgc
     3001 tgttgcttga agagatcatc gaaacaccgg gtctggtcga gcggctgctg gaggtgctgc
     3061 gccctgcggc aaagaggcgg tctgtgaagg agaaccagaa atgacgtggg aggattttta
     3121 tcttttttgt tttgcatttg gtctgtgtct cagcgcgatt gcatttgtag gcggcttctc
     3181 gcattttcac ctcggccacc tgcatgtgca tcacgcagga ggcgcaggcc atgcgcatgg
     3241 gggaagcggc cagtccatca accccatcaa tggctttacg ctgatgatct ttctgtgttg
     3301 gttcggagga acgggttatc tgctggagcg ttatggaggg ttcgttgttc ccgtagtgct
     3361 gttgctttct acgttggcgg gaattgcagg cgcaggagcg atcttctggt ttctggtgcg
     3421 tgtgctatta ccgcacgagc gcatgctgac cgcggaagaa actgcaactg cgggcatggt
     3481 ggcgcgtgtt agcgcgccta tccgcgacca cggaacagga gagatcttat tcacgcagaa
     3541 tggcagccgt aaattcgccg ccgcgcggag tgaaaacggc ctgccgattc cgcgtgacac
     3601 ggaagtagtg gtgatgcgct acgaacaggg catcgcatgg gtgcgccgct gggacgaagt
     3661 tgccggtgat atgaagtagt cctgcgcagg cgtgtaatga cgttgattca aacagtcttc
     3721 ccggtgagaa aggaccggga tcactatgac gaacacaatt atcgtaatag tcgggctatc
     3781 tcttctggca ctgatcattc tggttggttt gctggcgaag atgttccgca aggcgggtcc
     3841 gaatgaagca ctgattgtct atgggtttcg cgggcctcgc gtcatcaagg gccacggcac
     3901 ggtcatcttc cccatggtag aaagctgccg tgaactgtca ctggagttga tgtcatttga
     3961 cgtcgccccg cagcaggacc tttacacaaa gcagggcgtt gctgtcacgg tggaggctgt
     4021 ggcgcagatc aaggtgcgtt ccgaccagga gtctattctc actgcggcag agcagttcct
     4081 gaccaagacg cccccgcagc gcgagggtct gattcggctg gtgatggaag gtcatctgcg
     4141 cggcatcatc ggccagctta cggtcgagca gattgtgaaa gagccggaaa tggtctctga
     4201 ccgcatgcgt tccacctgcg ctgacgacat gagcaaaatg ggtctggagg tcgtctcgtt
     4261 cacgatcaag gaggtgcgcg acaagaacga atacatcacg aacatgggcc gccctgacat
     4321 tgcgcgcatt aagcgcgatg ctgatgtggc ggcggcagag gccgagcgcg atactgccat
     4381 tcgccgcgcg aacgcacagc gcgaggctgc gatagcgaag gccgcagctg accaggagcg
     4441 cgtgattgct gaaacggcat cgctggcaaa acaggccgaa gcccagcgtg atctcgacat
     4501 ccgcaaggcc cagtttgccg agcagagccg caaacagcag gcgcaggcag acaaggccta
     4561 cgaattgcag acaaatgtca tgcagcagca ggtggtcgct gagcaggtga aagtacagca
     4621 aatcgaaaaa gagcagcaga tcaaagtaca ggaagcggaa attcagcgcc atgaaaaaga
     4681 actcatcgca accgtgctga agcaggctga aattgagcgc cagcgcattg agaacctggc
     4741 tgccgccgaa aagtcgcgcc tgatgatcga ggctgaaggt caggctgctg cgattcgcgc
     4801 gcagggtgaa gctgaggccg cagtcatctt ccagaagggc gaggcagaag cgaaagcgat
     4861 gaacgtgaag gccgaagcct atcaggaatg gaaccaggcc gctgttgtag accgtctcat
     4921 cagcaacatg gcagacgttg tacgcgcgat gtctgagccg ttgtcgaaag tggacaagat
     4981 caccgttgtt tctactggca gcgatggctc agcgggagtc aacaagctga cgggggacat
     5041 cgcaaaaatt gcagcgcagg tcccggctct ctttgaggcg ctttccggaa tggacatgcg
     5101 cgagctgatg aacaacgtaa agacgatgcg ggcgcgcccg aacggcgggc ccactgtcga
     5161 aggtaagtaa cctcaaccgg acggggcctt gcgtcccgtc ccaatccttt taaggaggga
     5221 gatatggcac tcctggaacg cgttgcagcc ctgctgcgcg ccaacgttaa tgatctgatg
     5281 gacagggcag aggacccgga aaagatgatg aagcagcttg tcctcgatat ggagaaccag
     5341 cttcttcagg taaagacaca ggtagcgatt gccatcgccg atcagcatct tctggaaaag
     5401 aagaaaaaag aacacgacga cgaagcggct gaatggcaca agaaagcaga acttgcggtc
     5461 gccaggggga aagatgacct tgcccgcgct gcgctggaac gcgctcttgg gcatgaacaa
     5521 atggcctcag gctttgcgca gcagattgaa gaccagtcgg ctgaagctga gtctttgcgc
     5581 tccgccctgc gcaagcttga acagaaactg gcagaaaccc gcagtcgctg cgagatgttg
     5641 attgcccagc accgccgcgc ccgcgttgta aaccgggcca atcaggtacg gcaaaaaatg
     5701 gatgcaacca gcaacagcgc cagcatagac cgcatgaagc ttcgcgtgat tggaacagaa
     5761 tcagagaacg ctgccgtgca tgatttgctg aaagaagacg cgctggaaga ccgttttgca
     5821 gcattggaaa gggaagaccg catcgaacaa ttgttgcgcg atttgaaaga gcggcagggt
     5881 aagacggcct gagttttgcg aatcgaacag gagacctgta cctttatcac aagacatgga
     5941 ccattccaac ggatttgatc ttcaggcagc cgcgcgccag tctatgctgg accacggctt
     6001 tgaagctgga ttttctcagg ctgtaaaaga tgaagtggtc gcgattcagg agcatccgct
     6061 ctcggtagac ggctttgatg gcgtgcggga catgcgcgaa ttgccgtggt cttcgattga
     6121 caatgacaca tcgcgcgatc tcgaccagat tgaggccatc gcagaaatga atgacggcgc
     6181 tgttcgcgtc cgcgtaggca ttgcggacgt ggacgcatat gttcccaagg gaagtgcgat
     6241 agacaggttt gcaggcaaac agacgacaac ggtctacacc ggtgtgcaca acttctctat
     6301 gctgcccgag gagctttcga ccggactctc gtctctgctt gagaatgatg acaggttaag
     6361 cgtggtctat gaatttgttg tggatgcgca gggctgcgtg aattcgagcg caatttaccg
     6421 cgctactgtc aggaacaagg cgcagcttgc ttatccttct gttggagctt ggcttgaaac
     6481 cggcgcaaac cctcctgaga aggtcgccgc ttctgaagaa atcaagcaac agttacagat
     6541 acaaaattgc gtggcacagc ttttacgtag ccagcggtat aaacgcggcg cgctcaacct
     6601 ccagacgatt gagacgaacc cattgatgcg cgacggagcg ccggttgaga tccaggtgca
     6661 catgaggaac ccagccacgg acctcattga agattttatg atcgctgcga atgaagtggt
     6721 cgctacgagt cttgaaaaag caaagctctc ttccattcgg cgagttgtaa aaacgcccaa
     6781 gcgctgggac cgcattgtag agctggcagc gcagtacggc acaaaacttc cagatacgcc
     6841 tgatccaaag ccgttgcacg attttctctg cgaacggcaa aaggccgatc cggaccattt
     6901 ccccgacctt tcgctggcgg ttgtgaagct gctcggttcg ggcgaatatg ttctggagcg
     6961 cgcaggagaa actgctgaag gacacttcgg ccttgctgtt caggactaca cacactcaac
     7021 ggcccccaat cgccgctacg cagatttagt gacgcaacgg ctggtgaagg ccatgctcgc
     7081 gaaacagaag ccaccctatt ccgacgacga acttgcggcc atcgcccagc agtgtactcg
     7141 tcaggaagac gcggcccgaa aagtagagcg cgagatgcgc aaacgaattg cggctgtcgt
     7201 gatgcatgac cgcattggac agcagttcgc ggccattgtc accggagtga atgaacacgg
     7261 cacgtttgtc cgcactaaag cgccccatgt ggaaggaatg ctggtgcgcg gagcaaaggg
     7321 cgtggacgta ggcgacaaaa tccaggtcac cttgattcat accgatcctg ggcgcggcta
     7381 tatagatttt gcgcgcagct agaatgcaga catgcggccc catcttggaa cgcttgttga
     7441 tgatttcgag cggcacggca aagaaatcgc cattgttacg tatcgcggca atcgccgtat
     7501 tgttgctacg tatggtgaag tagctggcct tgcccggcgg tttgcggctt tgcttcggca
     7561 gcaggacatt gttgcgggag aacgcgtcct tatctggggg caaaactctg cggaatgggt
     7621 agcagcattt tttggctgtg tcctgcgcgg cgtaatggct gttccgttgg acgcagcctg
     7681 tactccggaa ttcgcaaaac gggtcttcga tgatacgcag ccaaagcttg tcgccgggga
     7741 cccaggccta ctctctcaat tgccggaagg tcctgcaaaa gtaatcctgg atgatctaaa
     7801 gacatcgctg ccgccggaac cgcagagccg tatcactgaa ccttcgctca gcctggatac
     7861 accactccag gtccttttca catcaggaac aacatcggag cccaagggtg tagtccatac
     7921 acatcgcaat gtgctggcaa gtatcacccc cattgaacgc gaaatgcaga agtatttgaa
     7981 atacgaacgg ccatttcacc cgctgcgatt tctgcataca ttgccgctga gtcatgtctt
     8041 cgggcagttc atgggcttgt ggctgccagt actgcttgct gcggaagtgc attttgaatc
     8101 gcgcttacag gcgcaacgac ttacagagat gatgcagcgg gagcgcatct cagtacttgc
     8161 tgccgtgcca cgtgtgttgg acctgctgcg cgcgcatttg cttgcgctgt tcccgcagtt
     8221 gcctgaacag attacgcggg cgcagggcga gcctgtatgg aagagatggt ggcgctttcg
     8281 taaggtccat cgccttctgg gatggaagtt ctgggcattt gtctgcggcg gcgcatcgct
     8341 tccagtggag ctggagtgtt tctggaacac gcttggcttt gcgctggtgc agggctacgg
     8401 aatgacggaa accaccgcac tgattacact caatcatccg ttcaaagtcg gcaagggcac
     8461 aatagggaag ccgctgcctg gccgcgatgt tcgcatctct gaggaaggcg aaattctggt
     8521 gcgcggcgat atggtttcaa ctgcgacatg gcagcgcggc cagcttatca ggtctgctga
     8581 cgaatggctt gcgacgggag atcttgcccg tagtgacgaa gagggccgtt ttcagttcct
     8641 gggcagaaaa ggccagatga ttgttacctc ctccgggctg aacattcacc ctgaagatgt
     8701 ggaagccgtg ctcgataagc agccaaacgt tcgcgcctct gtcgtcgttc cggcgaaaat
     8761 gccaggtgga acagaagcca tggcggtcct gctttttcga ggcgcagaac cggaagctac
     8821 ggaaaccatt cgcgcagcca atagacagct tgctgagtac cagcgcattc ggcactggcg
     8881 cctctggccc gggctcgatt ttccgcggac atccacagga aagatacagc ggggcaaggt
     8941 agctcagtgg gcgaactcag tctctggtga aaaggcgtct ccgcagcaaa atggcaggga
     9001 ttcgctgctt gatctgattg caacgattac gcacagccct gcaggcaaga ccacagatga
     9061 tgcgcgtctg gatgaagacc tgcacctcga cagcctgggc cgcgtgcagt tgcagggtga
     9121 gatcgaacag aagcttggca ttactctcga tgatgcgagc atgatcggca tggagaccct
     9181 tggcgagctg cgccgtgctt tgggaatgaa tggagaggac catcccggag ctttgcctga
     9241 atcttctggt gctcatccgc aacaccgtcc agcgaaatat atctatccgc gatggccgtg
     9301 gtcatggcct gtgcaaacgc tgcgctttat ctttcttgaa tgcgtagcgc ggccactgac
     9361 gcgctttctt gcaaagccac gtatcgcttg cgctccggaa tttgctgtgc cgcaaaggcc
     9421 agtgttgatc attgccaacc atgttacggc ctacgatgca gcgctggtgc tgtatgccct
     9481 tcccggcagg atgcgcaggc acgttgccat tgcaatggct gcggacatac ttgaggactt
     9541 ccgtcacgcg cgagggcagg ggacgtggat acaaaacgtg cttgctccca ttgcttactg
     9601 gcttatcact gcgctcttca atgtgtttcc gcttccgcgc agcgcaggtt ttcgcgagag
     9661 ttttgcgcat atgggacatg ccttggaccg gggatacaat gtccttattt ttcctgaagg
     9721 cacgcgcact tctggtcagt tgcaaaagtt ccgatccggc atcgggatgc tagttcagga
     9781 gtcaaacgca gccgtattgc ctgttgcttt gcgtggcctt ggtgaattaa aacaggcagg
     9841 caaaggttgg ttccgttcag gaaggcttga agtacgcacg ggtaagccca ttcagtttca
     9901 gccaacagat tcgccggaag gcatcactgc gaccctcgaa aatacgctgt gctcgatgct
     9961 ggaatgaata cgtctgtcgg ttacctcact tcgccattct gccaatgacc acatctatgc
    10021 ctccggtaaa actcctccct ggcatccgga cgtcttcaat ctcttcatac cccgtgttgc
    10081 tcaagttcga catttgcaga tatggatgta tgtggccctt ttctctggca atcgctgcgt
    10141 cccataccgg atacgggtct ttttgataac gctgtgtaat gccaacacgg ttgtgcagca
    10201 tgtatccgtg cagtggagcg atggtccatt ctgcaaatgc attgttgacg ggatagttga
    10261 agacgtactc cgattcaaga ccgtgcagtg cctgctgggc gccggaaagc agtgtccatg
    10321 cgagcctgac tcgctgattg tgcgcagcct gccatgtcca tgcactttcg acgcctgcaa
    10381 agcgcagccc agtcaggttg ctcgcatgcc acttgtcgaa ggcattcgcg cggacataat
    10441 caatcgcatc gtgctggcgc gaataaaatc cagttatcga aagataggac tgtgctcctg
    10501 caaaccagtc caggcccgct tcataattcc atgccgattc gggtttgagg ccaggatttc
    10561 ctaccgttgc cgggtcgctg tagtagagat cggtgtaact tggcagccga aagccatatc
    10621 cgaatgcaga ccgcagtttc aggctttcat tcagccaata gctgccagca agtgttggac
    10681 ttgtgacgta atgtccgcca ctgataattt cctgccgcag tcctgcggtg atagaagcgc
    10741 gcttccactg taagtcagcg tcgagatacc ccgcgcccca gttgcgcgca tgatttccga
    10801 gattgttgct gtgaatggaa tcaccatcgc cttctaatcc ataaaagagg gaactgcgcc
    10861 cgccaacgtg atcgctacga cgcagcgctc cctgccagct ttggtctatg tgctggtttt
    10921 tatagaacgc tgggtcgttg cgcagaagaa caaatatgtc agaatgcctg cgatatccga
    10981 aagcagctgt tgtctttgcg cctatttcct gacgcatgga ggcgaaccat cctttcgtgc
    11041 gctcccatga attgaagttg ccatagaact ggtcggcacc aaaggcgcgg tcgcttcctg
    11101 ccagcagaac gtcgctttca cccagcaaag tcttccatcg cgtttcggag ctcccgtttt
    11161 cattgcggta atcgcggtct gcgatgaatc cagtggagaa gttgcgtgcg cctgatgcga
    11221 gttctgacca gcgtttagcg gtaaatgatc cggtgaatgc ctgatcattt tcgccaaaac
    11281 tgcccacgcc gctgcgcagc ctcaatgcag tggtttccgg agatgccgta ataaagtcca
    11341 ccacgccgcc aatcgcatca gaaccataaa gcgtggagcc ggagccatgc agaatttcga
    11401 tggactgcat cgcgtccatc ggtacgggca ggtcgaggtt gtgatgggcc gactgtgcat
    11461 catttacgcg caatccattg atcagaacca gagtctgcga aaaagacgaa ccacgcatta
    11521 cgatgtccgc ctgagcgcca ccagcaccgc gttgttcaac aaacgtagat ggatctgtgc
    11581 gcaggcggtc ttccggaagc tgaaaagcaa gtgggtgctc ctgcgtgtcc ataatgacaa
    11641 ctgagcggtt tgattcgccc agtgtgactg gtgcggggtt gccaaccacg acaatcgtcg
    11701 tttttgccga agaggacgct tctgaattgt cctgcgctat tgctggcgcc gcgaaaagaa
    11761 ggcagcacaa aaaaagccaa agagctttca tgcgcttatc atcaggtggg tattgaacat
    11821 taacaatgtt aatttagtca tgaagtggat agttcatgga gtgaacattc cattaagctt
    11881 gaactatgcg gattgaaaca tttctggagc aaagccctct ttttgaaatc taccgggtat
    11941 cgcggaagct ggaggcgcaa ttgcgccaga ttctggggcc gaatgagatt tctttttttg
    12001 aagcacttgt gcttgtttcc attttgtttg aggaaccgca tgcggtaaga ccatcacatc
    12061 ttgcggaaac tttttccacc gggcgcggca acattagcaa ttgcatctcg tccctggaag
    12121 cgcgcgggct gttgaagcgc cgcattgatc cggaagatgg gcgcagcatt cagcttctgc
    12181 tgaagccgct gggccgcaag tgcgccatgg aggcagtccg tattttcgac cagatacaga
    12241 agcagtttga gaaaaagatc ggaatcatgc aactgaaagc cgcgctcagt gccgttcgtg
    12301 atgtcgagca tctctgtgaa gaagcagttt cctggatttg aaggttgagt ggccgatgac
    12361 aatcaatatc caccatctcg aattgctgat gttattgctg ttgttgtttg tagttctact
    12421 tgccgtgctg gcccgccgca ttgggcagcc gtatccgatt atttttgtgc tcggcggact
    12481 agtgctgagt tttctttctc cggtcccaga catagcgctg cgtccggatt ttgtttttct
    12541 ggtagtgctg cctccgctgg tgtttgcagc cgcccataat acgtcatggc gagattttcg
    12601 tgagaacatc atcagcattc tgatgctggc cttcgggctt gttgctttta ccgtcgttgg
    12661 cgtgggattt gccggccatt atttctttcc gcaactcacc tggaagacag gtctggtgct
    12721 tggcgcggtt gtttctacaa cagacacgct tgccgctacc agcatcgcgc gtaagcttgg
    12781 catccctgca cggattgtag acattctgga gggcgaaagc ctcatcaatg acgccagcgg
    12841 tctgctggcg gtgagtttta ccgttggcat tattaccagc ggacacgctc cctctttctt
    12901 tagcgcagtg ggccaactcc tgctgctttc aagcggaggc gtagtgattg ggcttgtgat
    12961 tgcatgggtg cattcgcggc tgatctaccg gattgaagct gcacaaattg aaatgaccat
    13021 gactctgatg acgccttacg tggcctatct ggctgcggaa cgcctgcata cttccggcgt
    13081 gatggcgacg gtggcctgcg gactttatct gggacatcat cgcgccaggc tgctttcgct
    13141 gggggcgcgc cttgaatcgc ccgccgtctg gaacaccgtc gaattcatat tgaatggtct
    13201 tgtctttctg ctgctgggcc tgcagatttc ttctgtgctc gcaggtattg agcacctggg
    13261 gaccggaagc ctgattgccg gtggtgtcat ggtgggtctg atcgtaattg cattgcgctt
    13321 tgtatgggtc tttccgggag ctcgaattgc gcaacggcta cgccggcgtt ttggcaaaga
    13381 agcgattgtc gagaaccggc aggtctttct ggtggcctgg ggcggaatgc gtggcgtgat
    13441 ctcgctcgct gctgcgcttt cgttgcctga atcgcttccg gacggcacgc cttttccagc
    13501 gcgcaacatc attatcttct ttaccttctg cgtcattctg atcacactcg tagggcaggg
    13561 gctgacaatg ccgtttatta tccgcaaact cgggcttgca caaagcggaa accttcccaa
    13621 tctcgaagaa cagatggcgc tgagaaggat gatccaaaat gcaattagtt atcttcagca
    13681 gcagaaagaa aaggattcag gcagtaaaga tgtatatgag gcggtagagc gggcctataa
    13741 gcggcgcatg gcccttttga agaaccctga agagggcgcc aaagaagagg tctcctcaca
    13801 ggcgcaccgt tatcttgaaa ttgcccgcgc ggcccgcaat gtggagcggc acacagcagg
    13861 ggaactgctg gcgacgaacc agatcaacga tctcgtctac cagcagctta tttttgatat
    13921 tgatttgcag gatgcacgct tctctgcgcc atttgcttct tagcagagaa gcgcagcagg
    13981 ccggctaaaa gctcaaccgc gcactcacct gaatctcgcg tccgggtgtc gtcacttccg
    14041 tgatcgagcc aaatccagct gtatttacaa agcggtcggg cgtgcccagg ttcgtgtggt
    14101 tgagggcgtt aaagaactcg ccgcgcgttt caaatttcag tgcctcggtc aggctgaagg
    14161 tgcgcactac ggcaaggtcc atggtctgca tgcctgggcc ataaacggaa ttgcgtcccg
    14221 catttccaaa cgtgtatgca ggcggggcct tgaatgctgc cggattgaac cacgtccgcg
    14281 cattgcgtgt cccgggacca aagaccggct ttccagtcac gttggcgcga atcggatttt
    14341 ctcccagcac cgtgccggcg ttggccgtat cgccaaagac agagatggtc agggggaaac
    14401 cggtctgcac ctgaaagacc gttgatgcgc gccagttctg cgttattccc cgggtaaacc
    14461 ggtccctcgt ccaggcagga agatcataga ctgcactcaa cgcaaagcgg tgacgcacat
    14521 cgcaggcggg gcctttctct gccttcaggt catagttgtt ttgcggcacg gccgcttcaa
    14581 acatgggcga acggaaatcg ggcgcattgg tcaggctttt ggaaaaggta tagtttgcca
    14641 gaagactgag tccacgggcg taacggcggc gcaggttcac atatcctgcg tcataccagc
    14701 tctgggccgt gttctcaaga tagttcatcg tgcttaccgg aaatgtcgtg ctggcgacaa
    14761 cggtgtttgc aggaaagacc gtattgggca cgaagctgat tttcgggaaa ggccgccggg
    14821 gctggatcgg gccaggtccg ggctgtgcgt tattgattaa gtgagagcgc tgtaaatgaa
    14881 atcctcctga accgagatat ccaatttcaa gtgtggtctc ttttcccagc gcctgttcga
    14941 cggaagcgct ccactgctga atgtactgcg gcgcaggatg cagcggcatt ccggtaaatg
    15001 tgaccgttac tttccccagt acaggttgag gaaaatcgaa accctggatg gaaggcgtaa
    15061 agttgtcact ctgcgccgtc tcaggaaaaa cataaggcac attgtggcgt tggttgcacc
    15121 aggtgttcat gtccaccggc gtgtagaaga tgccgtatgc tgcgtgcatc accagaccca
    15181 tgtgcggcac gctttgcgca attcctaaac gcggagcaaa atcggccttt ggcggataca
    15241 tcagtccctt gggatatcca ttctgtccgc cgacaaagac tgacggcacc ccatcactgt
    15301 taaagacaag attgctgttc gtataacgga tgtccaccag cgcgctcata tattcatagc
    15361 gcagcccgta attaagcgtg gtggtggaag tcatgcgcca ggtgtcctgc gcatatccgt
    15421 tggcatacca ctggcgcaga ttcatctgcg gaatgcctgc ctggccctgt cttgcaacag
    15481 gaaggcccag caggaagctg gcgagagcag agccagtgcc atcgtttgcg ccgatgtctg
    15541 tcgtgaagcc attggtgaac tggtaatagc cgcggttctg gaaaaatccc cacatgggcc
    15601 agataaattt ctgataggcc ccgccaaact tcatgctatg gcgtccgcgc tgccagttga
    15661 gggtatcgcg tccctcaatg actgtgtccc acgcatgcat gggcgtggcg ctgtagctgt
    15721 ctcccagggg ggagtaaccc tgcacattga aataaggtgc gccccacgct cccgggccgc
    15781 cgtatcctgt gccctggatg ccaagttcgc ttacgatgtc gttcttgttg gcattctctg
    15841 tcgtgtggtc catgctcagg cgcgagatgg ccagtgtggc catgtttaac atctgcggcg
    15901 taaaaatatg gttccacccc agaacaccct gttgagaaag gttgtcgtgc agatagccga
    15961 acccaggcag gttctcgggc ataaagccat gctcgccgcc cgcgctgtag cgaatgaagg
    16021 acgtgtcccc actggcgaaa tcatggtcaa aacgcagggt cccctggtcg gtatagtgga
    16081 cttcattgcg cacgtcgaga taattgttgg cgtcattgcc tgcgcctaca actgtaggct
    16141 ggcccatcat ggtcattccg cccatgttca tgcccatcat aatgttcggg cgcggcgcat
    16201 atttcgtcag catgattttt gctacagggc tgatacggtc cgggcagatg acattcatca
    16261 ttccattgca ctggaactgt tcgcgcgtaa actgcgggtt cgatgcactc accggaaggt
    16321 ttggattgta attggggttt ggttttgttg tcgcaggatc atagatattt accccgctca
    16381 tgctgaagtc accaccgact tcgtcctcag tcgggacggt gtctgtcatg gtcatggcgg
    16441 caatgtggcg cagtgcttca taattcacga agaaaaatgt acggcggctt ttgcccagca
    16501 gcggtcctcc gaaggcggcg ccgaaattgt tttgcacgag gtgcttgttt ccacccatat
    16561 cattaaagga gtgtgcgtcc attgcgccgt tgcgcagata ttcgtaagcg gtcccgtgaa
    16621 aggcagatga gccggaacgc gtgaccacat tgacctggcc gcctcctgct ccccccatat
    16681 cagccgagta gctgccaatc tcaatctgga attcctgcac cacatccggc gaagcgctca
    16741 ggttctgggt attgaaagta gggtctgtgt tggttgcgcc atcgagcaga aaataattcg
    16801 cattcgggcg agtgccgcca atactgactg ccgagcgctg gcctggccgc cagtaaagcg
    16861 gattcatatc acctgtctgc gcgccatgac tgacgtgcgc tccagggact gtgagcataa
    16921 ggtcaatcag catccgccca ttcagcggaa gctgttgtac tgacctgcgg tccacaacct
    16981 cacccacgct ggtgtcggtg gtcttgagtg tttccggaat ggccgttgct gtgactgtct
    17041 ctgtgcctcc cccgacctgc aactggacat caagcgttgc ttcctggccg acttccaact
    17101 gcaggcgctg gactgcctgc gcaaatccgg aatgcgtgac tgtgaccttg tattctccgg
    17161 gaagcagtcc cgcggcctca tacaggccgc tgtcgttcga tgtgacctca cgccgtgcac
    17221 ccgtatctat cgcggtaatc tcgacagtcg cccctggaat tgcatggtgc tgtggatcat
    17281 acacagaccc gcgcacgacg gcgtagttca cctgggcgcg cgctgcaaca gcacaaatac
    17341 agaagcagaa aacaaacaga aaaatcttgc gtgtcatggc tctcccgaaa gacaacaatg
    17401 ggccttacct ctcgtataaa tattgtgggg gaggcccagt cgaacgatat ctgcattggg
    17461 cgggcattgt cttgcatcag aagggcgaaa tgcgtctcca ccaaaaggtg gaggaaatca
    17521 gcccatcaga tgcggggccg caatactgat aaattcgcgc ggtgtgacca ccttcgtgct
    17581 tttccagaat tttggagaat gcttttggtt tccggtcatc agataatctg ccctggcagc
    17641 atcagcacat tcaagaaaga tgttgtcatc cgggtccctg gcaatcgcga gtttgcgctg
    17701 ggggagtaca gtgtggctgt gtttcttaat cagttgcagc aactgctgac gcacttcctt
    17761 ccgaatgcga agttcgggcc gtgcaagtag gctttggtat tcttcccaga ttggcttcag
    17821 gcaatctgga ggcggatggg gagcattctg cactgcccac ccatatctag ggttgatttc
    17881 cacagcgacc acaggatata gtcgctttac ctttgcggtg ccggatggtt ccactctctg
    17941 gctgaaaagg gaatccggtg taaatccgga gctgccccgc agcggtaagc aggaacgaaa
    18001 gcctcattca agcactggcc cgtcatgggc cgggaagccg aggcgagtag gtacgacaag
    18061 cctgcaagcc cgaagacctg ccgcagagaa acactcatgc gcctccgagg ggagtgccgg
    18121 gtgatgatgg ctttttctcc atcgttttcc tgttcagtct cacctctcag gcggaaagga
    18181 aatttccgtg cctgaattac agacaacgct cgctgatatc gcccctgact ttccgcaacc
    18241 ggaaccctct tcgcagatgc aggtccgcaa acgcaacggt tcgctggaac ctgtagatgt
    18301 caacaaaatc gtccgtgcgg tccagcgatg ctgccatggg ctgacgcacg tggaccccat
    18361 gcgcgtcgcc agccgcacca tcggagggct ttataatggc gcaaccacgc gcgagcttga
    18421 ctccatctcc atccagaccg ctgcatctct tatcgccgaa gagcccgaat actcaaagct
    18481 ggccgcgcgc ctgctgctgt ctacgatcgt gaaagaagtg tcggggcaaa acatctattc
    18541 gttttcgcag tccattgagc ttggtcatca ggaatcgctg attggccgtg caacatatgg
    18601 ctttgttgtg gaaaatgctc gcaaactgaa ccatgcagta gatgaagacc tctcggaccg
    18661 ctttgaatac tttggcctgc gcactgtgta tgaccgctat ctgctgcggc atcccatcac
    18721 gcgcagggtg atcgagacac cgcagtactt ctttatgcgt gtttcctgcg ggcttgcgct
    18781 tacggtcagc gaggccatag atttttatcg cctcatggcg acgcatgatt acatgccgag
    18841 ctcgcccacg ctctttaatt ccgggacgcg gcacgcacaa atgtcgtcct gctatttgat
    18901 tgattctcca aaggactcgc tggaagcgat ttatgacacc tataagcagg tggccatgct
    18961 ctcaaagttt tccggaggca tcgggcttgc ttttcaccgc gtgcgctcgg aaggctcttt
    19021 gattcgtgct accaatggca tttcgaatgg cattgttccg tggctccata cgcttgatgc
    19081 ttccgtggct gctgtaaacc agggaggcaa gcggaaaggc gcgtgctgcg tttatctcga
    19141 accctggcac gcagacatcg aacagtttct tgagttgcgc gacaatacgg gagatcacgc
    19201 gcgccgcacc tacaacctga accttgccaa ttggattcca gatattttta tgcgccgggt
    19261 agaggcggac ggaacgtggt cgctcttcga tccaaaagaa gttccgcatt ttcctgatct
    19321 ttatggcgaa gcatttgacc gggcctacga agaagccgag gcgcagaagc tctacgtaaa
    19381 gcagatcaag gcccgcgatc tctatgcgcg catggtgcgc acactggccg aaacgggtaa
    19441 tggatggatg acattcaaag acaccacgaa tctgaagtgt aaccagactg gaaggccaga
    19501 gaacgtcgtc catctctcga atctgtgcac ggaaatcact gaggtcacgt cctctggtga
    19561 gactgctgtc tgcaacttag gctcgatcaa ccttgcccgc cacatcacgc atggcagctt
    19621 cgattttgaa aagctcgccc gcactgtgcg cacggccatt cccatgctcg accgggtgat
    19681 tgacatcaac tactatccgg tggagaaagc agcttctgct aacagccgct ggcgtccggt
    19741 gggactcggc atcatgggac tgcaggatgt attctttcag ctcggcctgc cttttgattc
    19801 gtcagaagcc agcgtgcttt ccacgcaaat ccaggaagag atttactttc atgccttaag
    19861 cgcctcctgc gatctcgcag aaaaacatgg tccccatccg gcgttccatg agacccgcgc
    19921 cgcacaaggc aaattgcaat tcgatctttg gggtgtggtc ccgaaggacc ctgcgcgctg
    19981 ggatgcactc aaagaccgca ttcgtcaaaa cggactgcgt aattcgcttc tgattgcgat
    20041 agcgcctacg gccaccatcg catccattgt gggctgctat gagtgcatcg agccacaggt
    20101 atcgaatctt tttaagcgcg aaactctctc cggagaattt ctccaggtca atcgctatct
    20161 tgtagaagag ctgaaaaagc tcggcctctg gacggaagag atgcgggtgc gtatcaaact
    20221 tgcagaaggt tctgtgcaag gtatcgaaga gcttcctgag cacattcgca aaatttatcg
    20281 cacggtctgg gagctgccca tgcgcgccat cctggatatg gcggcggagc gcgctccctg
    20341 gattgaccag agccaatcgc tgaacctctt tgctgagtct ccgaacatcg gacgtctcag
    20401 ttccatgtat atgtatgcgt ggaagagcgg cataaaaacc acgtattacc tgcgctctcg
    20461 tcccgcaaca cgcattgcaa aaacaactgt gcagcaaagc gcctcggcag ttgcgtgctc
    20521 attggaaaat ccggggtcct gcgaagcctg ccagtaaaag agttctgctg cgacccaagc
    20581 ctactgaaga cttgagtggg ccaccacgtc aatcaaggag actttttatg tccatcgtta
    20641 ctgaatcatc cgtgaaagtg ccagactcca ttctcgatcc tgggctttgc ctcacattgc
    20701 ggcccatgcg ttatcccatt ttctttgaga tgttcaaaga tggaattcgc aatacctgga
    20761 ccgttgaaga agtagatttc tccactgacc tcaccgacct gcgctcaaag ctcacccctg
    20821 ccgagcggca tctcatccag cgcctggttg cctttttcgc taccggcgac tccatcgttt
    20881 ccaataacct cgtgcttaat ctttataagc acatcaactc tccggaagcg cgcctgtacc
    20941 tctcgcgcca gctatttgag gaggcagtgc acgtgcagtt ctatctcact ctgctggaca
    21001 attacattcc cgaccctgcg gctcgcgccg aggcctttgc cgctgttgaa aatatcccgt
    21061 ctatcgcaag gaaggcgcag ttctgcatga agtggatgga ctcaatccag caactcgata
    21121 cgcttcagac cgaagagcac cgccgccagt tcctgctgaa tctcatctgc tttgccggat
    21181 gcatcgaagg gcttttcttc tttgccgcct ttgcctatgt gtatttcctg cgcagcaaag
    21241 gccttctgca tggacttgca acgggcacga actgggtctt tcgcgatgaa agctgccatc
    21301 ttgagtttgc ctttgaagtc gtaaagacgg taagagagca gcacccgcat ctctttgacc
    21361 gggcaatgga agagcaggtc atcgccatgc tgaaagaggc tgtggattgc gaagcacagt
    21421 ttgccgaaga cctgctttca ggcggggtcg ctgggctctc cctcaaagat atgcgcgaat
    21481 atctgcaata tgtagccgat tcgcgcctgc agagacttgg tatccgtcca gttttcggct
    21541 caaagaatcc atttccattc atggatttgc aggacgtgca ggaacttacg aacttcttcg
    21601 agcgccgtgt ctctgcatat caggtcgcag tccagggaga agttgctttt cacgacgatt
    21661 tttaggccac ccatgccatg ggccttcagt ctaactaaag agaaaggagg ccctatggta
    21721 gcacttctcg gaatcatggc ggcaatcgtt tttctctgca ttatcgctgt gctctggagc
    21781 acaggtgtta tgacaacccg gcgctttaat ttcaggggca ggcaaaagtc ggaaggtcat
    21841 acacagccac cgcacccccg ggccagcgga gtgaactgag attttccctc cgcactttct
    21901 tatttgcgca ttccgagtta cagtagccct catacggaca tgagtgctac tgttgtctcc
    21961 aatccttctg ccgcaggaac aaagacccgt cttgtttcac tcgatgtgct gcgcggaatc
    22021 acgattgcct tcatgattct ggtgaataat ggcaatcaga attcctattg gccgctgcac
    22081 catattgact ggaatggctg gacgccaact gatcttgttt ttccaacctt tttatttgta
    22141 gccggagtct cactggtctt ttcctttgag tcgcggctca gtaagggcca atccaaagca
    22201 agtatctttg ctcatgccgt gcgccggtcc gccatattgt tcttactggg attggttgta
    22261 aatggctatc cttactttca tctcggcaca ctgcgtattt atggggtttt gcagcgcttc
    22321 gctgtctgtt tattgatggc cgcagtactg gagctgtggg accgtggggc aaagagcaaa
    22381 attgccgttc ttgcggcttg tctcatcgct tactggatca tcatgcgctg ggtcccggtg
    22441 ccggggtatg gtcttcccgg gcgcgacatt ccgtttctag ataaggatgc aaactgggtc
    22501 gcgtatcttg accggcatat cttccccggc aggctctatg agggtacgcg cgatcctgaa
    22561 gggcttctca gcaatctgcc cgccctgggc acggccattc ttgggatgtt gacggcgatc
    22621 tggttgcgct cggagaggac catccgcgca aagtgcctgg gtctgtttgc tgcggccatt
    22681 gcctgcctgc tgctgggcaa aatctgggga cactggtttc caatcaacaa aaaactctgg
    22741 accagctctt atgtgctgtt tgccggaggt tggtctttgc tcggtcttgc catttgctac
    22801 tggctgattg aaatcaaaaa gtggacgcgt ggctggacat tcccctggat tgtcttcggc
    22861 tccaatgcca ttgccgccta tgtcttttct gaactgctgg cgcctacctt gtggacattc
    22921 aaattcggcg ctgcgcacag atctataggt cagatgattt atcccagtgt cttcgcaaag
    22981 gttcccgatg cagcatttgg cgcgctgtta tactcgcttg ccttcgtcgc cgtgtgcttc
    23041 ctgccggtcc tggtgctgta caggaaaaag atcttcatca agatatagat caggccgggg
    23101 cccgcatcta ttttgagaga ataagtgggt gcccatttgt atcgccgtct gatgaacgaa
    23161 tggcgggaag gtcaagagaa aatttatgca ggaagcaact gccagaaaaa atactgaaat
    23221 cgtgcaaacg gattctcctg atgaagcgcc acaggccgag gtggttttgg agaaaccgcg
    23281 tggacggttc gttcgtggcg tgggcaaact aaagccgctc ctgccttatc tgaccggcgg
    23341 attgcgcatg attgatcatg gcgctgctca gattgtggcg cagcttctga gcctagccgc
    23401 caatgaaggc cccgcgtctg ctgaagcgcc ctctgaaata cagcaggaac ttgcagagat
    23461 tgaggccagt catcgcgatc tgcgcctctc cgtgcaggac cacagccttg aactcaagag
    23521 atttgaggac caaatcgtgc ttctgcgcca ggccgttgag cataacgctg ctgaacatgc
    23581 cgaagtggtg gaagaagttg attcactgcg caagctcatc ctgggcgtgg gaattggatt
    23641 gggagtcctt ctgatcgtgc tgattacctt cgtcggacta ttgctaattc gccatccata
    23701 aggcgcgaga ttttacacgg ccttcagagg atccagatcc agacccaaag ttgaacgaac
    23761 agggtcatcg tcctgctttg ttttatgttg tgaaattaaa tattaataaa attgaaataa
    23821 aaatcggccg gacagctaac cttttcttca tcacctgcaa aaaaaaaatt gccagttgtt
    23881 atctcaaagg actaggatac gtgtcagttc ggggtagtct gggcagtttc tgacacgcca
    23941 gaactgaaat gcagcagttt ttaatcgttt cacaggtacc cgatgtagat ccatttttaa
    24001 acaaagccag tgtacggagc gattccttat ggcatggtat gcctactgca ttacagagaa
    24061 gcaagccttt cctgaacttc ttcgtcatcg taaacccatt ccttttgaat ctatttgtgg
    24121 tattgcggga aaccaggtct ttttatatcc agcaagcgat ctggccgtca ttgtaagtga
    24181 acattctcct gacgatacac agcatgaacg cgctgctctt gagcatgccc gagtgattgc
    24241 ggactgcttc aaaacctcca ccgtgcttcc tttccggttt ggcactgttt tttctgacga
    24301 tgaaagtctt cgccggtcca tccggtccaa ccagcggcag tttttgacca acattgaacg
    24361 gcttcgcgga aaggccgaga tgcatctgaa agtgatgctc gatgattgtt gccgcgagca
    24421 gattcgcgcc tacccgtatt cgaacgggca ctctggaacg ggccgcgaat atctggcgca
    24481 actgcgtgaa aacgctactt tgcagcgcga acgccagacc agggcacggg ccgtttccgt
    24541 gcagatgcac cgcatgttct cccctcttgc agaagagatt acttgccggc gcactgattc
    24601 gggaaagatg ctgctcgata ttgcccacct gattgagcat aagtccgtag agcggtacca
    24661 gaacaaatac tcaagcgcgc tgcaattgat gaaagattgc cagatgcagt tgtccggccc
    24721 gtggccgcca taccattttg tgcaccggct caccacgcgc catcaccaca atggacatca
    24781 cgccacggcc cacgccgtcc aggcctgagg gcaaagacaa agccccggtg gacccacgaa
    24841 ccaccggggc ttctctcttc tggagcaaga gatcgaatca gcctgaacag ccctcgacct
    24901 ctatttagaa agacgcatca ctttaagaaa tgtttacaga aaatgcagat ctctccatca
    24961 acatggggag attgcaggtc tttacaagcc agacagattt acctcttaag caaatccact
    25021 ggttcattca cgtcgttggt acggaaatac tccgccagtg caggatgctg tttctttacg
    25081 gcctcataca tctcccaggc gacacggcga taagaaaagt gcccctgcgg ctgcgaccgc
    25141 agctctgaga tataaaccgc ctctgcgaaa tccattttga aaagagcgcg gatgcgggtc
    25201 ccaagcggaa gaacataatc tgcggcatcc gcggcctgcg cagcgctctt tgtgagttgg
    25261 gcgtgtgttg tgaaggccgc atccatggct gagttgtact gcggcagcag cccggcttct
    25321 gcaatgcctt caggaacatc atagccgtgg gcggtagtaa ggccttgtat gatctgcacg
    25381 cagcggcgat ggcggtgcat gtcgcggaag ccgccgatgt ccataaggat gtcaaaacgg
    25441 agtgcctgtc ccgcggcaaa ttcacgcaga agctcgtcgt gtctgccgcg atgcctcatg
    25501 cccagggtga tcagttcatt gcactttgct tcgtccagcg cagcaacatg gtcccggagc
    25561 tggcggtagg aataatggga aacgcgatag agcagtgtcg ttgcaatctc cacttccaga
    25621 gattccccgc gctcgactaa atccaccacc ggagcaggcg cgatggctgc atctttcatt
    25681 aactcccgcg ctgcctgtgc cagttcacgc cgggtttcga tttcatagcg attggcagcg
    25741 gcgtatttca ccagcgtggg tgcggttttg acttcgcggg tgagcagatt tgccgctttt
    25801 tcaccaagag cgggattgac agcggcaatc tcctgctgca atgccgtcag ttcttcccca
    25861 tggacgttcc aggcagcacc ggcggcagca gtccgcaggc gttcgcccag ttggcgaatt
    25921 tcagcatagg gacttgaaag caggcgtgag atttgcgttt ccagcgtgcg ggcattgacg
    25981 atttggccaa gggaggtatt ggtcgccagt gggagcagat agcgggccac atcgaatgcg
    26041 cgggcgcgaa gagtacgttc gtaactttcc ggcttcattt cggcgggtct gggtacctgt
    26101 gttttcaggt gctcgaacat gccttcggaa atggcctgat aagcgctgaa aagcgattca
    26161 atctgctgcg aaaactgttc tttttccgcc agattctgcc caaattcagg caaaaaccag
    26221 ccgcttttct tgaagttctg atagcgggtg gaccgttcct gcccgtccca gcgctgctcg
    26281 tctacaagag agatggctgc cagtagtgat agtctctcaa tggcgaaagc aatgtgcgcc
    26341 aggtccgcga tagagcggtg gccgtattgg aaataaaatg tgttgaggaa ctgttcggcg
    26401 cgctggctgc tgatctcttt gagcga
//
LOCUS       DASRBV010000033        26456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_52198,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000033 DASRBV010000000
VERSION     DASRBV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 26456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26456
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52198"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2801
                     /locus_tag="VFA02_01440"
     CDS             303..2801
                     /locus_tag="VFA02_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HZU08539.1"
                     /translation="MPKISRRRFLRDAALGSALLARQQRFAFASEIQANAAAVDWEQF
                     KLFYHRPAEVWPDALPVGNGRLGAMVFGNPAYERIQLNEESIWDGERRDRDNPRAGSA
                     VPAIRRLLFAGKAHEAEAMAVDDMLSIPRRMPCYQTLGDLYLDFSSGGLSRSDASEMT
                     DYRLELDLDRAIVAAKFTHNGIRHTREVFSSAPHQVIVVRLTTSKPGALSFTASLDRP
                     GNHETSISAPNELQLTGEALPVNDNPGLPDKERQAGIRFLARMRVLHKGGSLTQKIPS
                     QLQIHGATSATLLIDCATSFRFPAGEPAMNAAVARNLDNASRRSYSTLRTRHIADHRS
                     WFRRASIELGQSDPKRDMPTNERMDRVRAGEEDPGLLPIYFQFGRYLLISSSRPGTLA
                     ANLQGIWNDSVNPPWGSKYTVNINAEMNYWPAESTNLADLHTPFFDLLDMTREAGARV
                     AKTYYKASGFVVHHNTDIWGDAGPIDGLGGGIWPMGAAWMSTHLWEHFLYSRDMDFLR
                     NRAYPRLREIALFLLNYLTPSPDGHLVTGPSCSPENAYRLPDGTKANLCMAPAMDIEI
                     TRAIFQKATEAARLLETDAELCEKMKAAAERLPGFKIGRYGNLQEWQQDYEEVEPGHR
                     HISHLWALYPDDQITLRDTPELAQACRAALDRRLDHGGGSTGWSRAWIINCFARLENG
                     DRCHQQLMELLRHSTRDNLFDVCGMKKNSPYQIDGNLGAPAGMAEMLLQSHRGVIRFL
                     PALPSVWSDGQFAGLRARGGLTVDLTWRNGKAQKATLHAAIDHEHLLAAPSGRKIESI
                     SSRGKNVAFQPVGEGQYKLRVKSGERYEVRFVQE"
     gene            2845..5427
                     /locus_tag="VFA02_01445"
     CDS             2845..5427
                     /locus_tag="VFA02_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08540.1"
                     /translation="MPASAAVTVRTAPNATPRELYGAQQLRKTVAGIAENATILVAQR
                     NSPLLAHYDKQLATFWPNAEEAFILHRFGPTIIVTGYDASGTLYGALELADRISATHA
                     LPAALDYEDHPSLKLRAFCIGMQKPEITYEGAEYDYPYTPQNFPFFYDKSLWTRYLDM
                     LAGERINALFLWNGHPFTSLLKLPRYPEAQELSDAQLQQNIAMFRWLTAEADRRGIWI
                     IQGFYNIHLSHAFARAHHLPYHLSAPNPLATAYTRYAISEFIREYPHVGLMMTLGEAL
                     SPHEGATWLTQAIIPGVKDGLAEIHSAVQPPIVVRAHATDIDDDLKAALPLYSNIDTM
                     AKWNGESLTWTNVRGPILDKFKDLVSNSNIAIANIHLLSNLEPFRWGDPDFIRQIVLN
                     FQRIGIGGVHVYPLRYWDWPITADKTSPPLLQIDRDWLWFDAWGRYAWNPNRDPKKEQ
                     AFWAAQFADRFGTLAAGENLLRAYTLAGPCQPRLLPRIGITEGNRQAFALGMTMPELI
                     DAARFGPAETLWTGDAPKGERLSEYVEKEAKGLTHEGETPLQVADAVAEASAQALGAA
                     EAAAPSITKNRDEYQRIVNDMHAIHLLMLFYQAKTHAAEQVMLYGHDHNKEHLQKALP
                     LLEQSVADFQKLTELTDKTYRDAAALHTSQRRIPVTGGPGTLHWRNLLPVYQKELAIF
                     RSRLTNAGAPSFSQSEKDGKLQLLPQVAFTLAPDSKAQPFTLRSGEELQLSARKPFTI
                     ATVVPELQGMKGIRVDTRLHSPIHFTLSAPAQVLVAFLRGGPENRDAESWNPVIFNAL
                     TTAKNVPLTAWAKPLPAGDNDLDLSHGTGVVLGFVPQELHMQPHVAAALAGQPENLDW
                     LFDN"
     gene            complement(5451..7301)
                     /locus_tag="VFA02_01450"
     CDS             complement(5451..7301)
                     /locus_tag="VFA02_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZU08541.1"
                     /translation="MNKRSLTRREFLRSAVAVTAASASGPRFHTFAPEARARLQEFDY
                     RDVRLTGGPLKDQYDRIFASYLALDNDRLLKVYRQRAGLPAPGEDMGGWYDADGFVPG
                     HSLGQYISGLSRYACSTGGPLAYAKVQHLVEGFAETLGRDNNPFASPTASTTWPCYIL
                     DKHEIGLLDAYRLAGVQQAKALLPRVIHGALPYLPDHTYDRGPNSPKKAPYDETYILS
                     ENLFDSWELTGDPFYLDLAKKYLLNTEYYLPLAQGKDILPGKHAYSHVISLSSAAKAY
                     LVLGDPEYLEAIKNAWEMLETTQRYASGGWGPNETFVVPHDGKLFESLTATQNHFETP
                     CGFYAQAKLTRYLLCFTADARYGDSLERALYNTILGAKDPDGDGNYFYYSSYNPLAQK
                     SYYKKKWPCCSGTLVQSVADYVLNLYFHNHEGIYINLFAPSEVRWSCQGVPVIITQDT
                     AFPFGEDVSLRMKMPDAVEFSFFIRVPFWASSALKLRVNGKSAQDVSLHNGFARVRRR
                     WNTGDIIDFTLQRTLRTEAIDDKHPDTAAVMYGPLMMVAATPPAHLADRPLDLPRGLK
                     PLAYADAIFSYPFSATDVQFRPWFMLHDERYTTYFRQRQDEGLKVSSSWS"
     gene            complement(7294..8874)
                     /locus_tag="VFA02_01455"
     CDS             complement(7294..8874)
                     /locus_tag="VFA02_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZU08542.1"
                     /translation="MRIKEWPRWAGTALLLVQFVVSVHGQTASPALVSRLALVNQTVQ
                     SGPFYADWDSLGHYQVPAWFRDAKFGIFIHWGIYSVPAWGNEWYSRNMYVQGSPAYEH
                     HIATYGPQSKFGYKDFIPMFKAEKFDAEAWAGLFRRAGAKYIVPVAEHCDGFPMYASD
                     LTQWNAARMGPKRDVIAELARAARKQGLHFGASSHRAEHWWWHDGGAKFDSDVKDPGY
                     AGLYGPAQPMSLPGETSDKEPDPDHLEKWLPPDKAFLDDWLGRTTEIIDKYHPELLYL
                     DWWAGQPAFQPSLQRLAAYYYNRAALWKQGVVLTYKQHDFPENAAVLDIERGKLDSLR
                     LLPWQTDTSISIKSWGYVKDDQYRDARSLIDELVDVVSKNGNLLLNIGPKPDGTIPEE
                     EQRVLLQIGDWLRGNGEAIYGSRPWLVFGEGPTQDADSTAKGKDIKAYTAEDIRFTTK
                     GQRLYAIALGWPTSGKLTIHMLWKGTPYLRKPIAGIELLGTSGELHWEQTSTGLVIDL
                     PSTRPNDDAYVFRIRFHE"
     gene            9129..12626
                     /locus_tag="VFA02_01460"
     CDS             9129..12626
                     /locus_tag="VFA02_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08543.1"
                     /translation="MKSKIRFYFLFPLLAACMEAWAQVTSLGAIQGTVTDSSGAIIPA
                     ATVIVSNQATKAARTMTTSDKGVYVADALPGGDYTVTVSKPGFKQGVIQNIHLDPGQR
                     RGVDVSLSVGDVNTQITVNANTVQVQTESSESGGTISAKEVSNLMLNGRNFQTLAQIV
                     PGVSAVTGGGSLPQYGFGGYLGQTAVIVGGSSVEKSAYTIDGVYDADPSGLLNVNVVP
                     ELDAIAEFRILKSSYSARYGMAGSGQILVETKSGGNTFHGTGYEYLRNNQFATARPFI
                     STTANTPLHYHIFGYALGGPMIVPGVYNSDRTKNSYFFAAGEWRINHHYDGTHTRSMF
                     YQAMRNGDFSKSPTTPAKGLTLDANSQNLLASQGHNPATCITNGPDGRPDQIAMSCMD
                     PTAVALMNAYWPLPNATNPANLAAVNYTNTGTDSDTQNDQTYRIDQGIGKRNTVTGRW
                     MSEEVLDLRPRNYNDPAPVPTSKVYSRGLNAMLRVQTNISSSIINTAQIAETYSKWLL
                     GVSDYTLPSGASIAQQFPAADALNRIPDISLSGIWAWLGVGAQPNYNHTGEGIASDDL
                     SWVKGNHVLQFGALYIFGIRRANANAFPMGNFSFTGIHTGDPAADYLLGLNTSYTQSS
                     IQRSGVFHNRWGEAYFQDDWKALPRLTLNLGLRWTFLAPTTMSGAQVTNFDPSAFNPA
                     EAPVISTTGHETLNASNQPLTASGTVANPTNGLVFAGQNGTPYGFVKSRNYWGPRVGF
                     AYQLTNDGKTSIFGGYGLGYTPVAYEQITNLISNPPFVQSTTINNSLLSKPTIGVVGA
                     PPIPSLSVIGLQTRAVSTSTFSLTVEREVAPHAVASVAYAGNVTRHITLGGYDYNFPV
                     DGTSSTTPACAASASNPANGAYGTYAGPPVTHYSFDPCLNTGVVSSLYYRPYAGYSSI
                     SGSFSEGSGNYNSLQTGLVYRLTDLQLNMAYTWSKSLTNVIPGNPGANGAGVGYDEAA
                     SPQNPRNLAADYGLPDFDRTHVFTSAWVYQMPFQRHSTHILARELLSGWGTSGLAVLE
                     SGFAITPSLATAGTGLAARPNLIGKISHPGSGKPFDAKPYVSPAAFQPPAFGLFGNAA
                     PGTIRGPRDITFNVAADKNFPLTERVNFKLRAEAFNVFNHPNTVVTAVWSGTNGGSFG
                     TVNGAGDQRIMEFSGRFSF"
     gene            complement(12634..14685)
                     /locus_tag="VFA02_01465"
     CDS             complement(12634..14685)
                     /locus_tag="VFA02_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZU08544.1"
                     /translation="MKNLLLVLIVLINLPLAATTQKSGQETPPLLLGVAWYPEQWPES
                     RWDTDLSLMEAAHIHLVRIGEFAWSRIEPEEGKYDLDWMERAIALAAKHHIVVVIGTP
                     TDAPPAWMTQKYPDILRVNIDGHRDEHGNRRQFSYTSPRYLALCREVVERLAVRFGHN
                     PNVIGWQIGNEFSLDSYDSYTKHLFQQWLRNKYKTLDALNQHWTTAYWSQTYTQWDQI
                     PMEDTHGNPGLLLDHKRFVSDVWLHFEKNQITVLRAHIAPRQFITTNIGGLAWRDPWD
                     HYTIAQPLDLAAWDDYVGEGHVAPYKDGAMHDLIRGLKQKNFWVMEAQPGFVNWAPVN
                     NVLNRGEVRLMAWAAIGHGADAISYWQWRSALNGQEQYHGVLVGADGTPVPLYEEIKQ
                     IGEEFERIAPEFTGTSPQSDVAILHDYDSRWAIDFQPHTDRYDQLKVLLDYYRPLRDL
                     AQSIDVISPYVDLDRYKLVVAPDLNVIPDALGQHLLSYVQHGGHLVLGPRSGMKDAYN
                     ALDVHRQPGPLAGPLGGRVEQFYALEKSITAAGTWGNATVDIWAEALSTRNPATHVLM
                     RYGKSNGWLDGQPAVITRQIGKGSITYIGALFDAATMDAAARWMADESGVHPVFGPLP
                     PGVEVCRRVGQNSTIFILINHNPASVNVTLPHPMKDLLAGHTVTTVVLPVQGVAVLEE
                     R"
     gene            complement(14682..15458)
                     /locus_tag="VFA02_01470"
     CDS             complement(14682..15458)
                     /locus_tag="VFA02_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU08545.1"
                     /translation="MRRKIETSYSVPALDKGLDIVEALAAAQTPQSLTEIARTLKRSP
                     SELFRMLNTLEKRSYISRDPVSERYRLTLKLYELAHTHSPVEQLLRAASLPMRELAES
                     IHESCHLSVLSRQMLVVIAQEESPEPVRLSVEVGYRVQPLNTASGRVLIAFLGPQEQQ
                     YFLAGDAVYAEMTTSEKKALRRELEQIRANGHLMTLSTRRTGYDIACIAGNPETGVMA
                     ALAVPVIPGGTNAGKEKQLIPAVRKCAQQITAALGVSTPL"
     gene            complement(15488..16651)
                     /locus_tag="VFA02_01475"
     CDS             complement(15488..16651)
                     /locus_tag="VFA02_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZU08546.1"
                     /translation="MRPLEGLLIVDLSQFLSGPSASLRLADLGARVIKIERPQGGDLC
                     RQLYISNLELDGDSTLFHSINRNKESFAADLKNEEDREKLRALLRRADVMVHNFRPGV
                     IERLGFGYEAVSALNSRLVYAEITGYGKSGPWRDKPGQDLLVQALSGLTWLSGNADQP
                     PVPFGIAVADLYAGAHLAEGILACLVRRGVTGKGGRVEVSLLESVLDFQFEVLTTFLN
                     DGGKLPQRSAINNGHAYLAAPYGIYQTADGYLALAMNSITKLGELLDCKPLLHYTGPE
                     SHFEKRDEIKSILAGHLKTAPTQHWLSILEPADIWCADVLSWPQLMEQEAFQQLDMVQ
                     EVWRSNGISLKTTRCPIRIDGEILKSRRGAPRLGEQTEEIARDLISAAQGKNV"
     gene            complement(16639..17790)
                     /locus_tag="VFA02_01480"
     CDS             complement(16639..17790)
                     /locus_tag="VFA02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZU08547.1"
                     /translation="MNPQIELRGITWNHTRGYLPMVATAQRFLETHPDVSIQWDVRSL
                     QAFGDAPIEELVRRYDLLIIDHPFIGQAAENGLFLPLDEFLSCDFLDDQARNSVGASH
                     ASYQYEGHQWALAIDAAAPVSGWRPDLLAKSGFCVPATWSALLELARHGLVSIPAVPI
                     DSLMHFYMLCVALGGELFASEEQVAGVDAGCEALRMLRELLSFCGADCLRRNPFATWE
                     VLASSDSVAYCPFAYGYSNYARRGYAEHLLEFGGLVTLNDGTPLRSVLGGAGLAVSAH
                     CLHKEAAIEYAEFAASAVCQRTLYFDAGGQPGHRGAWLDAEANRRANGFFNKTLAVLD
                     AAWLRPRYPGYAEFQEQAAHIVHSYLQSGGSEKQVIQQLNHGARKICGL"
     gene            17877..18326
                     /locus_tag="VFA02_01485"
     CDS             17877..18326
                     /locus_tag="VFA02_01485"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZU08548.1"
                     /translation="MICEKYFEDYVVGERRQTSGRTITETDIVLHAGQTGDFYPHHMD
                     AEWCKAQPFGQRIAHGTLIFSMAVGMTAGEMNPRAMSYGYDRLRFLKPVFIGDTLHVV
                     VTVNEIKDDPKRPNHGIVVEHCDTMNQRKETVLVCDHLLLVEKRKID"
     gene            18612..22184
                     /locus_tag="VFA02_01490"
     CDS             18612..22184
                     /locus_tag="VFA02_01490"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08549.1"
                     /translation="MDAFFRKVLTASLFLIIPVCAWTQADRGSIAGKVTDTSGAVVPN
                     AAVDLKNEATQVTQSTFTNSAGDYIFQALNPGSYTLSISQPGFRQMERTHIVVDVNAT
                     NEQDIALEPGSTSQTVQVTAGVQNMSTTSASVGLVVEERSIQELPLIYGNPFSLEVLA
                     PGILPSGVNPNIHTYDSSTANVSVNGSALNSLEYRLDGAPDNRIRLSAYTPSTEMINQ
                     YRVETASYDATEGHSSGGFVNVSLKSGTDQFHGGAFVYYQNPSINANTWTIGNSSSAA
                     KPSWIREGADLGGPIWRQKAFFFFGWEHSRAGNPNVQVLTVPTLAERSGDFSALYALD
                     PKHPAGTSNTWQIYNPQSGHLCSSSGTEVCRNPFPNNQVTNISPIAQKILGYWPKPNA
                     TGNADGSNNYSYAASEPDYYYALAGRVDYTPTPSQNIFGHIVWSRRLQKGKNGYFYPV
                     SGTTLTYMNRGVALGYTWTISPATLLDSHLTWTRFVNQNVVSSQGILNATNLGMPDYV
                     VNGLPPAANAFPRIDATGYTSLNSDSGVLSHDDVTLASVQISHESGNHFLRAGFEYRM
                     YNTNGGSTTQSNGRYNSTGRYATANSNTSAQSIGQSLAELETGIVDSSAITINADFAS
                     RSNYMAGWIQDDWKATPNLVLNGGLRWEYEGPLSERNYKANTYFDFGATNPVAAPAQA
                     NYAKNASSYPSILPPAGQFQVNGGLRFAGQNGFGRQLYHSQILNLLPRVGLAYHFEQN
                     TVLHAGYGIFDDSLTTFYMSGGNAGSTTTFLLYQQGFSATTSQSATPDNGLTFPSTLA
                     NPFPNGIAQPTGSSLGLATFLGQSVSFQPMAPKTIYNQRWSMDMQHQFGAWLAMVGYV
                     GNHGVHLPVSRQFDAVPQQYLSRVTNAFDNTVYQRLVASVPNPFYKVLPSTVSLGSSK
                     TTSVAQLLLPYPEFTGVTAWMDTGMSNYHSLQAMLLRRFTNGASFTSAFTWSKTMDAT
                     QYLNNSDAKPWYGLSTNDRNFRLALSGIYEFPFGTGRRFLNQSNGVVRAIVSGWQVQG
                     LYQVQSGQPLSFNPESTSPVYTAGNPVQSAWGRSGYKKSIQPGAAGYWFNTANWLTST
                     SSSGCTTSICSGVLPNSYQVRNFPIRFDGLRSDFLDQLDAGLQRNFSVWRETQLQFRA
                     EAINALNHPVYSAPSTDWTNKAFGQITSQANQPRVYQFAAFLRF"
     gene            complement(22237..22323)
                     /locus_tag="VFA02_01495"
     tRNA            complement(22237..22323)
                     /locus_tag="VFA02_01495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22287..22289),aa:Leu,seq:cag)
     gene            22422..22670
                     /locus_tag="VFA02_01500"
     CDS             22422..22670
                     /locus_tag="VFA02_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08550.1"
                     /translation="MSRSRELPPIPTEQALHLRRLLHDLSNALEIILQTSYLLSTSNL
                     EGPALQWHQLLDQGVKQATNLNRELREYVRSHSEDAAA"
     gene            complement(22799..23227)
                     /locus_tag="VFA02_01505"
     CDS             complement(22799..23227)
                     /locus_tag="VFA02_01505"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZU08551.1"
                     /translation="MERTANAVWKGGLKDGKGVISTQSGVLKETQYSFTTRFENGIGT
                     NPEELIAAAHAGCFTMALSAQLGNANLTPESLETTASVKFEKTDAGFTITAIHLTTRA
                     KVPGASEAAFETAAQNAKTGCPISRLFKGNTEITLDAKLV"
     gene            23885..24235
                     /gene="hisI"
                     /locus_tag="VFA02_01510"
     CDS             23885..24235
                     /gene="hisI"
                     /locus_tag="VFA02_01510"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZU08552.1"
                     /translation="MSDTTIDFAKMDGLAPAIVQDAKTGQVLMVGFQNEASYQKTLET
                     GFVTFWSRTRQKLWMKGETSGNRLRVLEAATDCDRDTVLYRVEVEGDGLVCHEGTVSC
                     FTRPIALKAAQEAR"
     gene            24235..25110
                     /gene="hisG"
                     /locus_tag="VFA02_01515"
     CDS             24235..25110
                     /gene="hisG"
                     /locus_tag="VFA02_01515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZU08553.1"
                     /translation="MANKLKLGIPKGSLQDATIALFQRAGWNIYASGRSYFPAIDDPE
                     IECTLVRAQEMARYVDHGALDAGLTGIDWVVESGREVKSITSLTYAKQSRQKVRWVLA
                     VPDDSPYQKAEDLQGKTIATELVEVTRKYFAKKNVTVRVEFSWGATEVKPPTLADAIV
                     EVTETGSSLRANRLRIIDTVMESETHLIANPESYEDPWKKEKLDNIALMLRGAIAAQG
                     QVGLMMNVKKNDLANVLSVLPALTSPTVSALSDPNWVAVNTILEESVVRDVLPKLKAA
                     NATGIVEYPLNKVVL"
     gene            25110..26360
                     /gene="hisD"
                     /locus_tag="VFA02_01520"
     CDS             25110..26360
                     /gene="hisD"
                     /locus_tag="VFA02_01520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZU08554.1"
                     /translation="MRTVLTKGRDKLIAQLVARRNSVDERALPVAKRTIAGVRKGGDD
                     ALRRYAVRFDGITKQSQLRISQSELQQAWQGISPAFRNALETAAKNIRRFAQKQLPKE
                     WSFEQVPGLTVGQKISPLASVGCYVPSGRYPLPSTLLMTAIPAQVAGVPRIVAVSPKP
                     AKETLAAAAFLGITEFYRIGGAQAVAALAYGTESIPRVDKIVGPGNAFVTAAKKLVSF
                     DCAIDMLAGPTEIVVTSENGDPALIASDLVAQAEHDPDALAIFITTNKELAEKVSAGV
                     KERVRGNSIAAQSIKANGVIFVAGSVTEAREITNRLAPEHLTVDSESDLEWVQNAGSV
                     FVGRYSAQPLGDYISGPNHTLPTGGLARVRGGLSVYDFVKTITVQQYDRRALQALGPK
                     AITLAEAEGLKGHAEAIRARGARG"
BASE COUNT         6045 a         7691 c         7111 g         5609 t
ORIGIN      
        1 ccgtcaggcg agacaacaat gatgaccggg gacgtaagat tctcctgggc ggcacttcct
       61 tgcgcgaagg caagccctag ggccaataat gcgaactgta cgctgctgcg tatcattctc
      121 ttgtcgtact caacaacggg caaacatcat tgcggacaaa catctttgca gaaatcattg
      181 acaattttct tttattttat gttttatgtc tattatgaaa atatatttca caatcgcgga
      241 cgaatacaaa tacgttcgca acccaccctt ctgttacgca gagaaaagat aaaggtcact
      301 tcatgccgaa gatcagccgc cgccggttcc tacgtgacgc tgctctggga agcgctctcc
      361 ttgcccggca acagcgcttt gcttttgcat cagaaatcca ggcaaatgca gccgctgtag
      421 attgggagca attcaaactc ttctaccatc gcccggcaga ggtctggccg gatgcgcttc
      481 ccgtaggcaa tggacgtctc ggtgcaatgg tctttggcaa tcccgcatat gaacgtattc
      541 agttgaacga ggaatccatc tgggatggag agcgccgtga ccgtgacaat ccgcgcgcag
      601 ggtctgccgt gcctgcaatt cgcaggctgc tctttgcagg caaagcgcat gaagccgagg
      661 caatggccgt ggacgacatg ctctcgattc cccggcgcat gccctgttat cagacactag
      721 gcgacctcta tctagatttt tcttcaggcg gcctgtcgcg ttcagatgct tcagagatga
      781 cggattaccg tctcgaactt gatcttgacc gcgccatcgt agcggccaaa ttcacgcata
      841 acggcatccg gcacacgcgc gaagtcttct cctctgcacc gcatcaggtc attgtggtcc
      901 gccttactac aagcaaacca ggcgcacttt cctttactgc atcgctggac cgccccggca
      961 atcacgagac ctccatctcc gcgccgaacg aactgcaact gaccggagag gccctgccgg
     1021 tgaacgataa tcccggcctg ccggacaaag agcggcaagc cggtatccgc tttctcgccc
     1081 gaatgcgtgt gctgcataaa ggcggctcgc tcacacaaaa gataccatca cagttacaaa
     1141 tccacggggc cacatctgcc acacttttga tagattgcgc taccagcttt cgcttccctg
     1201 caggtgaacc agcgatgaac gcagccgtgg cccgcaattt agacaacgcc agccgccgca
     1261 gctactccac tctgcgcacg cgacacattg cagaccaccg ttcctggttt cgccgcgcaa
     1321 gcattgagct tggccagtct gacccaaagc gcgacatgcc cacaaatgag cgaatggacc
     1381 gcgtccgggc aggcgaagaa gaccctggat tgttgcccat ctactttcag tttggccgct
     1441 atctgcttat ctccagctca cgcccaggga cgctcgcagc aaacttacag ggcatctgga
     1501 atgactccgt aaatcctccg tggggctcaa aatacaccgt caatatcaat gcggaaatga
     1561 actactggcc cgccgagagt actaacctcg ctgatctaca cacaccattc ttcgatctgc
     1621 tggacatgac acgcgaagcc ggagcgcgtg tggcaaagac ctactacaag gcaagcggct
     1681 tcgtcgtcca tcacaacact gacatctggg gcgatgccgg cccaattgat ggcctcggcg
     1741 gcggcatctg gcccatgggc gcggcctgga tgtctaccca tctgtgggaa cactttctct
     1801 actcgcgcga catggacttt ctccgcaacc gcgcctatcc gcgtcttcgt gaaattgcgt
     1861 tgttcctgct caattacctt acgccttccc ctgacggcca tcttgtcacc gggccatcgt
     1921 gctcacccga gaatgcctat cggctgcccg atggcacgaa ggccaacctt tgcatggcgc
     1981 ctgcaatgga catcgaaatc acccgcgcca tctttcagaa agcgactgag gccgcgcggc
     2041 ttctggaaac agatgcagaa ctttgcgaaa aaatgaaggc cgccgctgag cgcctgccgg
     2101 gctttaaaat cgggcgctac ggcaatcttc aggagtggca gcaggactac gaagaagttg
     2161 agccgggaca ccgccatatc tcccacctgt gggcgctcta tcctgatgat cagatcacac
     2221 tgcgcgatac tccagagctg gcacaggcct gtcgtgcggc cctcgatcgc aggctcgacc
     2281 acggcggcgg aagcaccgga tggagccgcg cctggatcat caattgcttc gcgcgccttg
     2341 aaaacggcga ccgctgccac cagcagctca tggagcttct gcgccattcc acacgcgaca
     2401 atctcttcga cgtctgcgga atgaagaaga attccccgta tcagattgac ggtaatctcg
     2461 gcgctcctgc cggaatggcg gaaatgctgt tgcagagcca tcgcggcgtc atccggttcc
     2521 tgccggcgct gccttccgtg tggtccgatg gtcaattcgc tggcctgcgc gcgcgtggcg
     2581 gactcaccgt ggacctgacg tggcgcaatg gcaaggcaca aaaggccacg ctccacgccg
     2641 cgatagacca cgagcatctg ctcgcagctc cttcaggacg gaaaatcgaa tccatttcca
     2701 gcagagggaa aaacgtcgcc tttcaacctg taggagaagg ccaatacaaa ctacgcgtga
     2761 agagcggtga acgctacgag gtgcgttttg ttcaggaata aatctcaaat ttatcttctg
     2821 atcgtagttg tttttctatg cacattgcct gcaagcgcag ccgtcactgt ccgcactgcg
     2881 ccgaatgcca cgccacgtga actctacgga gcgcagcagc ttcgcaagac cgtcgcaggc
     2941 attgcagaaa atgccaccat cctcgtcgcc cagcgaaact cgccgctgct cgcacattac
     3001 gacaaacaac tggcaacttt ctggccgaac gcggaagagg ccttcatcct ccaccgcttt
     3061 ggcccaacga tcatcgtcac aggctatgat gcatccggaa cgctctacgg cgcgctcgag
     3121 ctggccgacc gcatcagcgc gacccatgct ttgcccgccg cgctcgacta cgaagaccac
     3181 ccttcgctca agctgcgcgc cttctgcatc gggatgcaga agccagagat cacttacgaa
     3241 ggcgctgaat acgactaccc ctacacgccg cagaacttcc ctttcttcta tgacaaatcg
     3301 ctctggacac gctatctcga catgctcgcg ggcgagcgca tcaatgcgct ctttctctgg
     3361 aatggccatc cgttcacttc gctgctgaag ctgccccgtt atcctgaggc gcaggagctt
     3421 tccgacgcac agcttcaaca gaacatcgct atgttccgct ggctcaccgc cgaggccgac
     3481 cgccgcggca tctggatcat ccagggcttc tacaatattc atctttcgca tgcctttgcg
     3541 cgcgcgcacc atctgccgta tcacctttca gcgccgaatc cgcttgcaac tgcatacacg
     3601 cgctacgcca tctctgaatt tatccgcgaa tatccgcatg tgggcctgat gatgactttg
     3661 ggcgaagcgc tctcaccgca tgaaggagct acatggctca cgcaggccat cattcccggc
     3721 gtaaaggacg ggcttgctga aattcactct gccgtccagc cgcccatcgt ggtacgcgcg
     3781 cacgccactg atattgatga cgacctgaag gccgccctcc cgctctacag caacattgac
     3841 acaatggcca agtggaacgg cgaatcactc acttggacaa atgtgcgcgg ccctatcctc
     3901 gacaagttca aagacctcgt ctcaaattcc aacatcgcca tcgccaacat tcaccttctc
     3961 tcgaatcttg agcctttccg ttggggcgat cctgatttca ttcgccagat cgttcttaac
     4021 tttcaacgca tcggcattgg aggtgtgcac gtctatccgc tccgctactg ggactggccc
     4081 atcactgccg acaaaacctc tcctccgctg ttgcagatag accgcgactg gctatggttt
     4141 gatgcctggg gacgttatgc gtggaatcct aatcgcgatc caaagaaaga gcaggcattc
     4201 tgggccgcac agtttgctga ccgcttcgga acgctcgctg ctggagaaaa tcttctgcgg
     4261 gcctacacgc ttgccggacc atgccagccc cgcctgctgc cgcgcatcgg catcacggaa
     4321 ggcaaccgtc aggccttcgc tctcggcatg actatgcccg aactcataga cgctgcgcgc
     4381 ttcggcccag ccgaaactct gtggacaggc gatgcgccca aaggcgagcg cctctctgaa
     4441 tacgtcgaga aggaagcgaa aggacttacg cacgaaggtg aaacgccatt gcaggtggcc
     4501 gatgcagtag ccgaagcgtc ggcccaggcc cttggcgcag cagaagccgc cgcgccgagc
     4561 atcacgaaaa accgcgatga ataccagcgc attgtcaatg acatgcacgc catccacctg
     4621 ctcatgctct tctatcaggc aaagacgcac gccgctgaac aggtcatgct ctacggacat
     4681 gaccacaaca aagaacatct gcaaaaggcg ctgccgttgc ttgaacaaag cgttgctgat
     4741 tttcagaagc tcaccgaact gactgacaaa acctaccgcg atgctgccgc cctgcacact
     4801 tcgcaacgca gaattcccgt caccggaggc ccgggcactc tccactggcg caaccttctg
     4861 cctgtttatc agaaggaact cgcaattttt cgcagccgcc tgacgaacgc gggtgcccca
     4921 tctttttcgc aaagtgaaaa ggacggcaaa ctgcaattac ttccgcaagt cgctttcact
     4981 ctggcgccag attcaaaagc acaaccattc acactgcgta gtggtgagga actacaacta
     5041 agcgctcgca aacccttcac catcgcaacg gtcgtacctg agcttcaggg catgaaaggc
     5101 attcgcgtag ataccaggct gcattctccc attcacttta ctctttctgc gccggcgcaa
     5161 gtcctggtgg cctttctccg cggcggccct gagaacaggg acgccgaaag ctggaacccg
     5221 gtgatcttca acgccctcac cacggcgaaa aacgtcccgc tcacagcctg ggccaaacct
     5281 ctgcctgccg gagacaacga tcttgacctc agtcacggca ccggagttgt gctcggcttt
     5341 gtgccacagg aactgcacat gcaaccacat gtcgcggcag cactcgccgg acaaccagaa
     5401 aatcttgatt ggctttttga taactgatga acgccggtac atcctcccat ttatgaccag
     5461 gatgatgaga ctttaagccc ttcatcctgc ctctgcctga aatacgtcgt gtaccgctca
     5521 tcgtgaagca tgaaccacgg acggaactgg acatccgtcg ctgaaaatgg ataggagaag
     5581 atggcgtcgg catacgccag cggtttcagg ccgcgcggca agtccagagg acggtctgcc
     5641 agatgagcag gtggcgtggc tgccaccatc atcagcgggc catacataac ggcggccgtg
     5701 tccggatgct tatcatctat cgcttctgtt cgcagagtac gctgcagcgt gaaatcaatg
     5761 atgtctccgg tgttccacct gcggcggacc cgagcaaagc cgttgtgaag agatacgtcc
     5821 tgtgcgctct tgccattcac gcgcaacttc aatgcactgc ttgcccagaa cggcacccga
     5881 atgaaaaagg aaaactcaac ggcgtcaggc attttcatcc gcaaacttac atcttccccg
     5941 aacggaaacg cagtatcttg cgtgatgatg acgggaactc cctggcagct ccaacgtact
     6001 tctgatggcg caaagaggtt gatataaatc ccctcgtggt tgtggaaata cagattcagc
     6061 acatagtccg ccacactttg cacgagcgtt cctgaacagc atggccattt ctttttgtaa
     6121 tagctctttt gggcgagagg gttgtaactg gagtagtaaa agtaattgcc gtcgccatcc
     6181 gggtcttttg ctccgagaat ggtgttgtag agagcgcgct cgaggctgtc accatagcgg
     6241 gcgtctgcgg taaaacacaa aagatagcgt gtgagtttgg cctgtgcata aaagccacag
     6301 ggcgtctcga aatggttctg ggttgctgtg agactctcga acagcttgcc atcatgcggc
     6361 acgacaaatg tttcgttcgg tccccatccg ccggaggcgt agcgttgcgt cgtttccagc
     6421 atctcccatg catttttgat ggcttcaaga tattcaggat ctccgaggac gagatatgct
     6481 tttgcagccg aactgagcga gatgacatga ctgtaggcgt gtttgccggg aagtatgtct
     6541 ttgccctgcg ccagaggtag atagtattcc gtgttcagca gatatttctt tgcaagatcg
     6601 agatagaagg ggtccccggt cagttcccat gagtcgaata ggttttctga gagaatgtat
     6661 gtctcgtcat aaggggcctt cttcggactg ttggggccgc ggtcataggt atgatcgggc
     6721 agatagggaa gtgccccatg gatgacgcgt ggcagaagtg ctttcgcctg ctgtactccg
     6781 gcaagcctgt aagcatcaag caggccgatt tcatgcttgt ccaggatgta gcacggccag
     6841 gtagtagaag cggtggggct ggcaaatgga ttgttatcac ggcctaaagt ctcagcgaat
     6901 ccttccacca gatgctgcac tttggcataa gcgagaggcc ctcctgtgga acacgcataa
     6961 cgggacaggc cggaaatgta ttggccgagc gagtgaccgg gcacgaagcc gtcggcatcg
     7021 taccagccgc ccatgtcttc tccaggcgca ggcaggcccg cgcgctgccg gtagaccttg
     7081 agcaggcggt cattgtcgag tgcgagatag cttgcgaaga tgcggtcata ctggtctttc
     7141 agcggaccgc ctgtcagccg cacgtcgcga taatcaaact cctgcaggcg cgcgcgagct
     7201 tccggcgcaa aggtatgaaa gcgaggccca gacgcgctgg ccgcggttac agcgactgcg
     7261 ctacgcagaa attcacggcg cgtaagagaa cgtttattca tggaaacgaa tccggaaaac
     7321 gtaggcgtca tcattgggcc tggttgaagg taagtcaatc actaatcctg tgcttgtctg
     7381 ctcccaatgc agttcgccgg acgtgccaag caattcgata ccggcgattg gtttgcgcag
     7441 ataaggcgtg cccttccaga gcatgtggat ggtgagcttt ccgctggtcg gccagcccaa
     7501 agcaatggca tagagtctct ggcctttggt ggtgaagcga atgtcttcgg ccgtgtatgc
     7561 tttgatgtcc ttgccttttg ccgtgctgtc ggcgtcctgt gtgggccctt cgccgaatac
     7621 gagccacggg cgtgagccat agatggcttc tccattgccg cgcagccagt cgccgatctg
     7681 caacagcact cgctgttctt cttcaggaat ggtgccgtcg ggctttgggc cgatattgag
     7741 cagcaggttt ccattcttgc tgacaacatc caccagttcg tcaatcagag agcgggcgtc
     7801 gcggtactgg tcgtctttga cgtagcccca ggatttgatg ctgatagatg tgtccgtctg
     7861 ccacggaagc aggcgaagcg agtcgagctt gccgcgctca atgtccagca ccgcggcgtt
     7921 ttccgggaaa tcatgctgtt tgtacgtcag cacaacacct tgcttccata atgctgcgcg
     7981 attgtagtag tacgcggcca gccgctgaag agacggttga aaggcaggct ggcctgccca
     8041 ccagtccaga tacaaaagct ccggatgata cttatcaatg atctctgttg tgcgccccaa
     8101 ccagtcatcc agaaatgcct tgtccggagg cagccacttt tccagatggt ctgggtcagg
     8161 ctccttgtca ctggtttcac cgggcaggga cataggctgc gcaggaccat acaagcctgc
     8221 atatccgggg tctttcacat cagaatcaaa ctttgctcca ccgtcgtgcc accaccaatg
     8281 ctccgcacgg tgagaggacg ctccgaagtg cagtccctgc tttcgcgcag cacgggccag
     8341 ttcggcaatc acatcgcgct ttggccccat ccgcgcggca ttccattgtg tcaggtcaga
     8401 agcatacatc gggaagccat cgcaatgctc agccaccggc acgatatatt tagctccggc
     8461 gcggcggaag agtccggccc atgcttcggc atcaaacttc tcagctttga acatgggaat
     8521 aaagtccttg tagccaaact ttgactgcgg gccgtaagtt gcaatgtggt gttcatatgc
     8581 agggcttccc tgcacgtaca tattgcggga gtaccactca ttgccccagg cgggaacaga
     8641 gtagatgccc cagtggatga agatgccgaa ctttgcgtcc cgaaaccatg cagggacctg
     8701 gtaatgccca agagagtccc agtctgcgta aaaagggccg ctttgcactg tctggttgac
     8761 cagagcaagc ctggagacaa gtgcagggga tgcagtctga ccatgtacgg agacaacaaa
     8821 ctgcaccaga aggagcgcgg tcccagccca gcggggccac tccttaattc tcattagtaa
     8881 aaacattttc tattgacatc ccaattttca acgtactaca gttttgccgg gttggagaac
     8941 gcaattctgc atgttctgaa tttctgagga ccggacagca gataaatctt gcgtcctgcg
     9001 tccctccagg aacaaggacc acccgttcgc caggaaaaat cagatgtaga catagaagca
     9061 aatattcgtg caaatgcgtt atgaaaatta attttatata caaaaattta agagagaggc
     9121 cggccatcat gaaaagtaaa atccgcttct acttcctgtt ccctctgctg gctgcctgca
     9181 tggaggcctg ggcgcaggtc acgagcctcg gcgcgattca gggcactgtg acggactctt
     9241 ccggcgcgat cattcccgcc gctacagtca tcgtttccaa tcaggcaaca aaggccgcgc
     9301 gcaccatgac aacgtctgac aaaggcgtat acgtggccga tgctcttcca ggcggcgact
     9361 ataccgtcac cgtctcgaag cctggattca aacaaggagt cattcagaac atccaccttg
     9421 acccgggcca gcgtcgcggt gtggatgtct cgctctccgt cggtgacgtg aatacacaga
     9481 taaccgtcaa tgccaacaca gtccaggtgc agacggaatc gtcggagagt ggaggcacca
     9541 tcagcgcgaa ggaagtctca aacctgatgc tgaatggacg caactttcag acgctggcgc
     9601 agattgttcc cggagtcagc gcggtgaccg gaggaggcag tctgccgcaa tatggctttg
     9661 gcggatatct ggggcaaacg gctgtcattg ttggaggttc ttctgtcgag aagtctgcgt
     9721 acacgattga tggcgtctac gatgccgacc ccagcgggct gctcaatgtg aatgttgtgc
     9781 ctgagttgga tgcaattgcc gaattccgca ttctgaagag cagctacagc gcgcgttatg
     9841 gcatggccgg ttccggacag attctggtag aaaccaaaag cggcgggaac accttccacg
     9901 gcacgggcta cgaatatctg cgcaacaacc agttcgctac agcgcggcct tttatctcga
     9961 cgaccgccaa cacgccgctg cactatcaca tttttggata cgcgctgggc ggtccgatga
    10021 tagtgcctgg cgtttataac tctgaccgca caaagaacag ctatttcttt gccgcaggcg
    10081 agtggagaat caaccaccac tacgacggca cacacacgcg ctcgatgttc taccaggcca
    10141 tgcgcaatgg cgacttcagc aaaagcccaa ccacgcccgc aaaaggactc acgctggatg
    10201 caaacagcca gaatctgctg gcaagtcagg gacacaatcc tgccacctgc atcacaaacg
    10261 gcccggacgg caggcccgac cagatcgcaa tgtcctgcat ggaccccacc gctgttgctc
    10321 tgatgaatgc ttactggccg ctgccgaatg cgacgaatcc cgcaaacctt gccgctgtga
    10381 actacaccaa cacgggcacg gacagtgaca cgcagaacga ccagacgtac aggattgacc
    10441 agggcattgg aaaacgaaat acagtcaccg gacgctggat gtcagaagaa gtgctcgacc
    10501 ttcgtccacg taactacaac gaccctgctc cggttccaac ctcgaaggtc tacagccgcg
    10561 gcctgaatgc gatgttacgc gtgcagacga atatttcatc gagcatcatc aacacagcgc
    10621 agatagccga gacctacagc aaatggctgc tcggtgtttc tgattacact ctgccctccg
    10681 gagcgagcat cgcgcagcag tttcctgcag cagatgctct caaccgcatt ccggacattt
    10741 cgctgtccgg catctgggcc tggctgggag tgggcgcgca gcccaactat aaccacacag
    10801 gcgaaggcat tgcatcggac gacctgagtt gggtaaaggg aaaccatgtc ctccaatttg
    10861 gagcccttta tatcttcggc atccggcgcg cgaatgcgaa tgccttcccg atgggcaact
    10921 tcagctttac tggtatccac accggcgatc cggcggcgga ctatctgctt ggtctgaaca
    10981 caagctatac ccagtcgagc atccagcgca gcggcgtttt tcataatcgc tggggcgagg
    11041 cctactttca ggacgactgg aaggcccttc cccgtctgac actcaacctg ggtctgcgct
    11101 ggacttttct tgctcccact actatgagcg gcgcgcaggt gacgaatttt gatccatctg
    11161 cttttaatcc ggccgaggcc ccggtcatca gcacaacggg ccatgagacg ctcaatgcca
    11221 gcaaccagcc gctgactgct tccggcacag ttgcaaatcc aacgaacggc ctggtgtttg
    11281 ccgggcagaa cggcacgccc tatggctttg tgaagagcag gaattactgg ggaccgcgtg
    11341 tgggttttgc ttatcaactt acgaacgacg gcaagacctc aatttttggt ggctatggtc
    11401 tgggatatac gcctgttgct tacgagcaga ttacaaatct catctcaaac ccacctttcg
    11461 tacaaagcac aaccatcaac aacagcctgc tcagcaaacc gacaattgga gtagttggag
    11521 cgccgccgat tccatcgctt tctgtaatcg gtctacagac aagagcggtc tctacttcca
    11581 ccttcagcct gactgtggaa cgggaagtcg cgccccatgc cgtggccagc gtggcctatg
    11641 cgggaaatgt gacccgccac attactctcg gcggctatga ctacaacttt ccagtggatg
    11701 ggacctcctc tacaacacct gcctgcgcag ccagcgcgtc gaacccggcc aatggagcgt
    11761 atggcacgta cgcgggacct ccggtcacac actactcttt cgatccctgc ctgaataccg
    11821 gagtggtatc aagcctgtat taccgtcctt atgcgggata ctcgagcatc tccggctcgt
    11881 tcagtgaggg ctcgggaaat tacaattcgc tccagacggg actggtgtat cgcctgacgg
    11941 acctgcaact gaacatggct tatacctgga gcaaatcgct gacgaatgtg attcccggca
    12001 atccgggcgc aaatggagca ggtgtgggct atgacgaggc ggccagcccg cagaatccgc
    12061 gcaatcttgc agccgattat ggtctgcctg attttgaccg cacgcacgtc ttcacttcgg
    12121 cctgggtgta tcagatgccg tttcagcgcc actccaccca catacttgcg cgcgagctgt
    12181 tgtccggttg ggggacctcc gggctggcgg tgctggaaag cggcttcgcc atcactccca
    12241 gcctggccac tgccggaaca ggtctggcgg cacgacccaa cctcattggg aagatttcgc
    12301 atccaggcag cggcaagcca tttgacgcaa aaccctatgt cagccccgcg gccttccagc
    12361 cgccggcatt cggtcttttt ggaaatgccg ctcccggcac cattcgcgga ccgcgcgaca
    12421 ttacatttaa cgtagcggca gacaaaaact ttccccttac agagcgggtc aacttcaaac
    12481 tacgcgcaga agcattcaat gtgttcaatc atcccaatac tgtggtgact gcggtgtgga
    12541 gcggaacgaa cggcggctcc ttcggaaccg tgaacggcgc aggcgaccag cgcatcatgg
    12601 agttttccgg aagattcagt ttttaagtgc gtgtcaacgc tcttccagca cggcgacccc
    12661 ctgaacaggc agcaccacgg tggtgaccgt gtgcccagca agcaggtcct tcatcggatg
    12721 cggcagtgtt acattgaccg acgcgggatt gtgattgatc aggatgaaga ttgtgctgtt
    12781 ttggcccact ctccggcaca cttccacacc cgggggcaga gggccaaaga ccggatgcac
    12841 accgctttcg tccgccatcc atctggctgc tgcatccatg gtggctgcgt caaaaagtgc
    12901 accaatatag gtgatgctgc ccttgccaat ctgacgggta atgacagcgg gttgtccatc
    12961 cagccagcca ttactttttc cgtagcgcat cagcacatgt gttgccgggt tgcgagtgct
    13021 cagggcttct gcccagatat ccactgtcgc attgccccag gtccccgctg cggtaatgga
    13081 cttttccagt gcgtagaact gctcgactct cccgccgagc ggtccagcca gagggccggg
    13141 ctgacgatgg acatcgaggg cattgtaagc atccttcatt ccggaacgcg ggccgagcac
    13201 aaggtgtccg ccatgctgaa catagctgag taagtgctgg cccagagcgt ccggaataac
    13261 attcaggtcc ggcgctacga ccaacttata ccggtcaagg tcaacatagg gactgatgac
    13321 atctatagac tgggcaaggt cacgcagcgg acggtagtaa tcgagcagga cctttagctg
    13381 atcataacgg tccgtgtgag gctggaaatc aatggcccag cggctgtcat aatcgtgcag
    13441 gatggcgaca tcggattggg gagatgtacc tgtgaattcc ggagcaatgc gctcgaattc
    13501 ttcgccaatc tgttttattt cctcataaag aggaacaggc gtcccatctg ctcccacaag
    13561 tacgccgtgg tattgctcct gtccgttgag ggcgctgcgc cattgccagt agctgatggc
    13621 gtcggcgccg tggccgatgg ctgcccatgc catcagccgg acttctcctc gattgagaac
    13681 attgttcacc ggggcccagt tcacgaagcc gggctgtgct tccatcaccc agaagttctt
    13741 ctgcttcaga ccgcgtatca ggtcgtgcat ggcgccatct ttatacgggg ccacatggcc
    13801 ctcacctaca tagtcgtccc acgcagcaag gtcgagcggc tgcgcgatag tgtaatggtc
    13861 ccaggggtct cgccaggcca gcccgccaat gtttgtagtg atgaactgac gcggggcaat
    13921 gtgcgcgcgc agcactgtaa tctggttctt ctcaaaatga agccagacat cgctcacaaa
    13981 acgcttgtgg tcaagcaaaa gcccgggatt cccatgtgta tcttccatgg gaatctggtc
    14041 ccattgcgta taggtctggc tccagtaagc ggtggtccag tgctggttca gagcatcgag
    14101 tgtcttgtac ttattccgca gccactgctg aaacagatgt ttggtgtagc tgtcatagga
    14161 atcaagagaa aactcatttc caatctgcca tccaatcaca ttcggattat ggccgaaccg
    14221 gacagccagc ctctcgacga cttcacgaca gagcgctaga taacgtgggc tggtatagga
    14281 aaactgccgg cggttgccat gctcatccct gtggccgtca atattgacac gcaggatgtc
    14341 cggatatttc tgcgtcatcc atgcgggcgg agcatcggta ggcgtgccaa tgactacgac
    14401 aatgtggtgc tttgctgcca gagcaatagc gcgctccatc cagtcaaggt catacttgcc
    14461 ttcttctggt tcaatacggc tccatgcaaa ctcgccgatg cgcaccagat gaatgtgcgc
    14521 ggcttccatc aggctgaggt ctgtgtccca gcgcgattca ggccattgct ccggatacca
    14581 ggcaacgcca agcagcagag gcggggtttc ctgtccggac ttctgtgtcg ttgcagccag
    14641 cggaaggttt atcagcacaa tgagaacaag aagtaggttc ttcataaagg cgtggaaaca
    14701 cccaatgctg cggtaatttg ttgtgcgcat tttcgtacag caggaatgag ttgcttttct
    14761 ttgccggcat tggtcccgcc gggaatgaca ggcacagcga gggccgccat cactccggtc
    14821 tcggggttgc cggcaataca ggcgatgtcg tacccggtgc ggcgggtgct gagcgtcatc
    14881 agatgtccat tggcccggat ctgctccagc tcacgccgca gcgccttctt ttccgacgtg
    14941 gtcatttccg catagaccgc atctccggcc agaaagtact gctgctcttg tggcccgaga
    15001 aaggcaatca gcacacggcc cgaagcagtg tttaagggct ggacgcgata gcctacctca
    15061 acagaaagcc gcacaggttc cgggctttct tcctgcgcaa tcacgaccag catctgccgg
    15121 ctcagcacgc ttaagtggca cgattcgtga atgctctcgg ccagctcccg cataggcagg
    15181 cttgcagccc tcagcagttg ttccacagga gaatgcgtgt gcgcaagctc atagagcttc
    15241 agcgtaagac ggtaacgttc agaaaccgga tcgcggctga tataggagcg cttctccaga
    15301 gtgttcagca tacggaaaag ctcacttgga ctgcgcttca gggtcctggc gatttcagtc
    15361 agcgactgag gtgtttgcgc cgcggccagg gcctccacga tgtcgagccc tttatcaagg
    15421 gccggaacgg agtagcttgt ttctatcttt cttcgcatag tgtccgtaag caagtgtact
    15481 gaagctgcta cacatttttt ccctgtgccg cagatatcag gtcccttgca atttcttcag
    15541 tctgttcgcc cagacgcggc gcgccccgtc tgctcttcaa tatctctcca tcaatgcgaa
    15601 tcgggcagcg cgtcgttttc agtgaaatgc cattgctgcg ccacacctcc tgcaccatat
    15661 ccagttgctg aaaggcctcc tgctccataa gctgcggcca gctaagcacg tctgcgcacc
    15721 agatgtccgc tggttcgagg atcgagagcc aatgctgcgt gggtgctgtt ttcagatggc
    15781 cggcaagaat gcttttgatc tcgtcccgtt tttcaaagtg tgactcagga ccggtgtagt
    15841 ggagaagcgg cttgcagtcc agcagttctc cgagctttgt aatggagttc atggccagcg
    15901 caagatagcc atccgctgtc tgataaattc cgtaaggcgc agccagatat gcatgtccat
    15961 tgttgattgc gctgcgttgc ggcagctttc cgccatcgtt cagaaaagtg gtcagtacct
    16021 cgaattgaaa atcaagaaca gactccagca ggcttacctc aacgcgtccg cctttccctg
    16081 tgacaccgcg ccgcaccagg caggccagaa tgccttcggc cagatgcgcg cctgcatata
    16141 aatctgcgac cgcaatccca aagggcaccg gaggctggtc tgcatttccg ctcagccagg
    16201 tcaggccaga gagcgcctgt accagcaggt cctgtccggg cttgtcccgc catggaccgg
    16261 acttgccata accggtaatc tccgcataca ccagccgcga gttcagcgcg ctgacagctt
    16321 catatccaaa gccaagccgt tcaattacgc cgggacggaa gttgtggacc atcacatcgg
    16381 cgcgccgaag caaagcacgc agcttctcgc ggtcctcttc gtttttcagg tctgccgcaa
    16441 aactttcttt gttgcggttg atggagtgaa agagcgtgct gtctccatcc agctcaaggt
    16501 tggagatgta aagctgccgg cagaggtcgc cgccttgtgg ccgctcaatc ttgatgacac
    16561 gcgcccctaa atcagccagc cgcagcgatg cggaaggacc agacaaaaac tggctcagat
    16621 caacgataag aagtccttct aaaggccgca tattttcctg gctccatgat tgagctgctg
    16681 tatgacttgt ttctcgctgc cgccagactg gagatagcta tggacaatat gcgcggcttg
    16741 ttcctgaaac tctgcatacc cgggatagcg tggccgaagc catgcggcat ccaggactgc
    16801 aagggtctta ttgaagaagc cattcgcccg gcgattcgcc tctgcatcga gccatgcgcc
    16861 gcgatgcccc ggctgcccgc ccgcatcaaa atacaaagtg cgctggcaaa cggcgctggc
    16921 tgcaaattcc gcgtactcga tggctgcttc cttatgcagg caatgcgctg aaaccgcaag
    16981 tcctgcgccc cccaggacag aacgaagcgg cgtgccgtca ttcaatgtaa ccaatccacc
    17041 aaattccaga agatgctcgg catatccacg gcgggcatag ttcgaataac catacgcaaa
    17101 aggacagtac gcaacagagt cgctggaggc gagcacttcc caggttgcga aaggattgcg
    17161 gcgcagacag tctgctccgc aaaaactcag caattcccga agcatgcgca gcgcttcgca
    17221 tcctgcatcc acaccggcca cttgttcctc tgaggcgaac agttcgcctc ccagcgccac
    17281 gcagagcata tagaaatgca tcaggctgtc aatcgggact gcgggaatgc tcaccaggcc
    17341 atgtctggcg agctccagca atgcgctcca ggtagcaggg acacagaagc cggacttcgc
    17401 aagcaaatca ggccgccatc cactgaccgg ggccgctgcg tctatggcca gcgcccactg
    17461 gtgcccctcg tactgatagc ttgcatggga cgcgcctaca gaattgcgcg cctgatcatc
    17521 cagaaagtcg cacgaaagaa attcgtccag cggcaaaaac aggccgttct ccgccgcctg
    17581 cccgatgaaa ggatggtcta tgatcagcag gtcataccgc cgcaccagtt cttcaatcgg
    17641 agcatcgcca aaggcctgca aagagcgcac gtcccactga atcgagacgt ccggatgtgt
    17701 ctcaagaaaa cgttgcgccg tagccaccat gggaagatac ccgcgtgtgt ggttccaggt
    17761 gatgccccga agctcaattt gaggattcat gattctctac atctggattc atcatatttt
    17821 atatgcaaaa ataaatttca taaagatgag attgcaatac agaggggtgg gaactgatga
    17881 tttgcgaaaa atattttgaa gattatgtgg tgggcgaacg ccgacagacc agcggccgca
    17941 cgattactga gaccgacatt gtccttcatg ccggacagac aggcgatttc tacccgcacc
    18001 acatggacgc cgaatggtgc aaagcccagc ccttcggcca gcgtatcgcg cacgggacgc
    18061 tgattttcag catggctgtt ggcatgacgg ccggagagat gaatccccgg gccatgtcat
    18121 atggatacga ccggctgcgc ttcctcaaac cggtctttat cggggacaca ttacatgtgg
    18181 tcgtaacggt gaacgaaata aaggacgacc caaagcgtcc caatcatgga atcgtagtgg
    18241 agcactgcga cacgatgaat cagcgtaaag agacagtgtt ggtctgtgac catctgttac
    18301 tggtggagaa acgaaaaata gattgaagtc ggaaggggcc cgccgtcgtc gtatgcggat
    18361 caacctcact cttaatacgc agcacatatg gactgaccac aaggccccgg aacatttcaa
    18421 tttgccggca gtatctccac ttgtaatttc atcttgacaa gcctttcttt accgcactag
    18481 cattcggttc gattcttcca ttgagagaag aaaagcgatt tatgcttcct gtgcaggaac
    18541 aactttaact caatagtggt ctgacctctt ttgagaaaag gagagggggc ccattttgaa
    18601 cgaggctagc aatggacgca ttcttccgga aagttttgac ggcatcacta tttctgatca
    18661 ttccagtctg cgcgtggacg caggcggacc ggggcagtat cgcaggcaag gtcacggaca
    18721 catccggcgc tgtggtcccc aacgcggcag tggacctgaa aaacgaagcg acgcaagtga
    18781 cgcaaagcac gttcaccaac agcgcaggcg attacatttt tcaggcgctc aatcctgggt
    18841 cctacacgct ctccatttcg caaccgggat tcaggcagat ggagcgcacg catattgtgg
    18901 tggacgtaaa tgccaccaat gagcaggaca ttgccctgga gccgggctca acttctcaga
    18961 cggtgcaggt cacggccggc gtccagaata tgtccacaac gtcggcatcg gtcggcctgg
    19021 ttgtagaaga gcggagtatt caggagctgc cgctcatcta cggtaacccg ttctccttag
    19081 aagtgcttgc gccgggtatt ctgccttcag gtgtgaaccc aaacattcat acgtatgaca
    19141 gcagcacggc aaacgtctct gtgaatggct cggcgctgaa ctcgctggaa taccgcctgg
    19201 acggcgcccc tgataaccgt attcgtcttt ccgcttatac gccttccacc gaaatgatta
    19261 accagtatcg tgtggagacg gcgagttacg acgcgaccga aggacactcg tctggcggat
    19321 tcgtaaatgt gagcctcaag tcgggaacag accagtttca tggcggtgcc tttgtttact
    19381 atcagaaccc cagcattaat gcgaacacct ggacgatagg gaattcaagt tcagcggcca
    19441 agccttcatg gatacgcgaa ggcgccgacc tgggcggccc aatctggcgg cagaaggcat
    19501 ttttcttctt tggatgggag cacagccgcg cgggcaatcc gaatgtgcag gtgctgaccg
    19561 tacctactct ggctgagcgc tcgggcgatt tttcggccct ctatgctctg gacccgaagc
    19621 atcccgccgg gacctcgaat acatggcaaa tctacaatcc gcagagtggg catctttgca
    19681 gcagcagcgg aacagaggta tgccgcaatc ccttcccgaa caatcaggtg acgaacatca
    19741 gcccgattgc gcagaagatc ctcggctact ggcccaaacc caatgcgaca ggaaatgcag
    19801 atggcagcaa caattattcc tatgcagctt ccgagccgga ttattactac gctctggctg
    19861 ggcgcgtgga ctatactccg actcccagcc agaacatctt cggacacatt gtatggagcc
    19921 gccgtttgca aaagggcaag aatgggtatt tttatcccgt ctcaggcacg acgctgactt
    19981 acatgaatcg tggcgtggcg ttagggtata catggaccat ttcacctgca acgctcctgg
    20041 attcgcacct gacctggacc cggtttgtaa accagaacgt agtgagttcg cagggaatac
    20101 tcaatgcgac caatctgggc atgccggact atgtggtgaa tggccttccg cctgcggcaa
    20161 atgctttccc gcgcatagat gccacaggct acacttcgct gaattcagac agcggagtgc
    20221 tttcacatga cgacgtgacg ctggcttcgg tccagatcag ccatgagtca ggcaatcact
    20281 ttctgcgcgc gggatttgaa taccgcatgt acaacacaaa cggcggctcg actacgcagt
    20341 cgaatgggcg atataactcg accgggcgct atgccacggc aaacagcaac acttccgctc
    20401 agtccattgg gcaaagcctg gctgaactcg aaacaggcat agtggacagc tccgccatca
    20461 ccatcaatgc cgattttgct tcacgctcga attacatggc gggttggata caggacgact
    20521 ggaaggcgac tccgaacctg gtcttgaatg gagggctgcg ctgggagtat gaagggccgc
    20581 tctcagaacg taattacaag gccaacacgt atttcgattt cggggcaacg aatccggttg
    20641 cggcgccagc gcaggccaat tatgcgaaga acgcatcgtc ctatccttca attctgccgc
    20701 ctgccggcca gttccaggta aatggagggc tgcgctttgc gggtcaaaat ggattcgggc
    20761 ggcaactcta ccactcgcag attttgaatc tcctgccgcg ggtcggcctc gcgtaccatt
    20821 ttgagcaaaa cacggtcctc catgccgggt acggcatctt cgacgattcg ctgacgacgt
    20881 tctatatgtc aggcggcaat gctggttcca cgacgacctt cctgttgtac caacagggtt
    20941 tcagcgcaac gaccagccag agcgcgacgc cggacaacgg ccttaccttc ccgagcacgc
    21001 ttgcaaatcc gtttccgaat ggcatagcgc agccgacagg aagctcactg ggactggcaa
    21061 ctttcctggg acagtccgta agctttcaac cgatggcacc gaagaccatt tacaaccagc
    21121 gctggagcat ggacatgcag caccagtttg gagcatggct ggcgatggtt ggctacgttg
    21181 gcaatcacgg agtgcacctg cccgtaagcc ggcagttcga tgccgttcca cagcaatatc
    21241 tctcacgggt caccaatgcg tttgataaca ctgtctacca acggctggtt gcaagcgtgc
    21301 cgaacccttt ctacaaggtc ctgcccagca cagtctcgct gggatcatcg aagaccacga
    21361 gcgtggccca attactgttg ccgtatccgg agtttaccgg tgtcacagca tggatggaca
    21421 cgggcatgtc caattaccac tctctgcaag ccatgttgtt acggcgcttc accaatggag
    21481 catccttcac ctcggccttc acctggtcga agacaatgga tgcgacacag tatctgaaca
    21541 actccgatgc aaagccgtgg tacggccttt caacgaatga tcgcaacttc cgtctggcgc
    21601 tttcaggcat ctacgagttt ccattcggga caggcagacg cttcctgaac cagagcaatg
    21661 gcgtagtgcg cgcgattgtg agcggatggc aggtgcaagg gttgtaccag gtgcaaagcg
    21721 gccagcctct ctcgttcaac ccggagagca caagcccggt ttacaccgcc gggaatccgg
    21781 tgcaatcggc atggggccgc agcggataca agaaatctat tcagccagga gcagcgggct
    21841 actggttcaa tactgccaac tggctcacaa gcaccagctc ttccggatgc acaacgagca
    21901 tctgctccgg cgtgctgccg aactcatatc aggtgcgcaa tttccccatt cgctttgatg
    21961 gattgcggtc tgatttcctg gaccagctcg atgccggctt acagcgaaat ttctccgtct
    22021 ggcgcgagac acagctccag ttccgcgctg aggccatcaa tgccctgaat catccggtct
    22081 acagcgcgcc gagtaccgat tggacaaaca aagcatttgg ccagatcact tcgcaggcca
    22141 accagcctcg cgtttatcaa ttcgcagcat ttctgcggtt ctgatggagg gcagccggaa
    22201 aaagcaccct gctattgctt tgaggaggaa aacgattggt gccgaagaag ggactcgaac
    22261 ccccacaccc ttgcgggtac atggacctga accatgcgcg tctgccaatt ccgccacttc
    22321 ggcaagggta ggaacaaaag cagcaattct tagattaagg agtcggggaa gatgtgtcaa
    22381 acttcgtcag cacttgtatc atcagcagtg agaggaaatg aatgtccagg tcacgagaac
    22441 tgcctcccat cccgaccgaa caagcgctcc acctgcgccg ccttctgcac gacctgagca
    22501 acgctcttga aattatcctg caaacaagtt atctgctcag cacttcaaac ctggaaggcc
    22561 ctgcactgca atggcaccag ctcctggacc aaggtgtaaa acaggccaca aatctgaatc
    22621 gcgaattgcg cgaatatgtc cgttcccaca gtgaggacgc cgcggcataa aaacatccca
    22681 ccgtgtggga aagccagtac ctctctcgtg ttctccgcgg tcaggaaact ccggtacagg
    22741 ctaccctgat gcatccgcgc aagccagaaa ctcgcgcgga taccttcagg accagttttt
    22801 acaccagctt tgcgtccagc gtaatttccg tgttgccctt gaagagccgc gaaatcgggc
    22861 atccggtctt ggcattctgc gcagccgtct caaaggccgc ctcactcgcc ccgggcacct
    22921 tggcgcgcgt ggtcagatgg atggccgtga tcgtaaatcc ggcatcggtc ttctcaaact
    22981 tcactgaagc tgtcgtctcc agcgactccg gagtcagatt ggcatttcca agctgtgcgc
    23041 tcaacgccat ggtgaagcat ccggcatgcg ccgctgcaat caactcttcc ggattggtgc
    23101 caatgccgtt ctcaaaccgc gtagtgaagg aatactgtgt ctctttcaaa acgccgctct
    23161 gggttgaaat tacgcctttg ccatctttca ggccgccttt ccagacagca ttcgctgtac
    23221 gctccataac tgctcctttt tcatagagaa tttctgtgcg cgtatgcgca ggtctgcgac
    23281 tttgaggata tcaatgccgc taaggttgcg tcgaatatac ctcagggcct gtaaagggcc
    23341 ggttaacact atcagggcca acagcgacaa gaacggatta accgggtgcg cgaagcaaac
    23401 cgcggcgaat accggctgta tttttgcggc gagcgacaaa gcagtcggaa aatccggcca
    23461 tatttctttg ttgtccccga ctgtagtccc gccacaccct tcggattcct cctccggttc
    23521 ccagcaacag atctccgccg ccgggttggt cctcgtctgg cctgattttc cctcgcagcg
    23581 ctgccgttct gattgtgtta acacaccctt agatgctgca cacttctatt gtgtggtatg
    23641 acagcaagca ttaacggtgt tgacatcttc agcttctgct tataacctca atcacagatg
    23701 cggattcatc gccatcatcg tcatcaccat catcacgtga cgagcgtgtc ggcgaatcct
    23761 aatggcctga agtaaaagcc agaaatcaag gaatcctaaa gcccgccgac gcgccaagcg
    23821 caggcgggct ttttgcatct gcccgcaggc gcaaggcccc ggcaagatat aaaaggaaaa
    23881 gcagatgtct gacacaacaa ttgattttgc caagatggac ggtctggctc cggcaatcgt
    23941 gcaggacgca aagaccgggc aggtgctgat ggtcggcttc cagaacgaag ccagctatca
    24001 aaagaccctc gaaaccggat tcgtcacctt ctggagccgc acccgccaga agctctggat
    24061 gaaaggcgag accagcggca accgcctgcg cgtgcttgaa gccgccaccg actgcgaccg
    24121 cgatacggtc ctctatcgcg tggaggtaga aggcgacggc ctggtctgcc acgaaggcac
    24181 cgtaagctgc ttcaccagac ccattgcttt gaaagctgcg caggaggccc gttaatggca
    24241 aataaactca agctcggaat cccgaaaggc agcctgcagg acgctactat cgctctcttt
    24301 caacgggcag gatggaacat ctacgccagc gggcgctcct attttcctgc gattgatgat
    24361 cccgaaattg aatgcaccct cgtccgcgcg caggagatgg cccgctacgt ggaccacggc
    24421 gcgctcgatg ccggactcac cgggattgac tgggtcgtcg aaagcggacg cgaagtaaaa
    24481 agcatcacca gccttaccta tgccaagcag agccgccaga aagtgcgctg ggtacttgcc
    24541 gttccagacg actcgcccta ccagaaggcc gaggaccttc agggcaagac catagcgacg
    24601 gaactggtcg aagtcacaag aaaatatttc gcaaaaaaaa atgtaacagt gagggtcgaa
    24661 tttagttggg gcgcgacgga agtgaagcct cccacgctgg ccgacgcgat tgtggaagtc
    24721 accgagaccg gaagctctct gcgcgccaat cgcctgcgca ttattgacac cgtcatggaa
    24781 agcgagaccc acctgattgc caatcctgaa agctacgaag atccctggaa aaaagagaaa
    24841 ctggacaaca ttgcgctgat gctgcgcggt gccatcgccg ctcagggaca agtaggcttg
    24901 atgatgaacg tgaagaagaa cgacctggca aatgttcttt ccgtgctgcc tgcgttgact
    24961 tcgcccaccg tctcagcatt gagcgatccg aactgggttg cggtgaacac gattctggaa
    25021 gagagtgtag tgcgcgatgt gctgccgaag ctcaaggcgg ccaatgcgac cggaattgtg
    25081 gagtatccgc tgaacaaggt ggtcctgtaa tgcggaccgt gctaacaaag gggcgcgaca
    25141 agctgattgc gcagctcgtc gcgcggcgca acagcgtgga tgaacgcgcg cttcccgtgg
    25201 caaaacgcac cattgcaggc gtgcgcaagg gcggagacga cgccctgcgc cgctacgctg
    25261 ttcgctttga cggaattacg aaacagtcac agttacgtat ttcgcaaagc gaactgcaac
    25321 aagcgtggca agggatttca cctgcattcc gcaatgccct tgaaaccgcc gctaaaaaca
    25381 tccgccgctt cgcgcaaaaa caactgccaa aggaatggag cttcgagcag gtcccagggc
    25441 tgacggtggg ccagaaaata agcccgcttg catccgtcgg ctgctatgtc cccagcgggc
    25501 gctatccact gccgtctacg ctgctgatga cggccattcc cgcgcaggtc gcaggtgtcc
    25561 cgcgcatcgt tgccgtctct ccaaagcctg caaaggaaac gctcgcagct gctgcctttc
    25621 tcggcatcac ggagttttac cgcatcggcg gagcgcaggc cgtcgccgcg cttgcttatg
    25681 gcacagagag cattccgcgc gtagacaaga ttgttggtcc gggaaacgcc tttgtcactg
    25741 cggccaaaaa gcttgtttct ttcgattgcg cgattgacat gcttgccggg ccgacggaga
    25801 tcgtggtcac cagcgagaac ggcgacccgg cgctgattgc ctcggattta gtggcacagg
    25861 cagaacatga tccggacgca ctggccattt tcatcacgac gaacaaagaa ctggcggaaa
    25921 aggtcagcgc gggagtaaaa gagcgggtgc ggggaaattc gattgccgcg cagtctatca
    25981 aggcaaacgg cgtgatattt gtagctggtt cagttacaga agcgcgagag atcaccaacc
    26041 gcctcgcgcc cgagcacttg accgtagatt cggagagcga cctggaatgg gtgcaaaatg
    26101 caggttcggt ttttgtgggc cgctactcgg cgcagccttt gggcgactat atctccggcc
    26161 cgaaccacac tttgcccact ggcggactgg cgcgcgtgcg cggcggactt agcgtctatg
    26221 actttgtaaa gaccatcacc gtccagcagt atgatcgcag ggccttgcaa gcgctggggc
    26281 caaaggccat tacgctggct gaagcagaag gactgaaagg acatgcagaa gcaattcgtg
    26341 caagaggcgc ccgtggctga gcttatccgg ccgcgcgccg ccgttctgcg cacaaaggaa
    26401 tatcacccgc cgcttggctc gcgcgacggc gtgcgtttgg attttaatga gaacac
//
LOCUS       DASRBV010000034         6547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_102836, whole genome shotgun sequence.
ACCESSION   DASRBV010000034 DASRBV010000000
VERSION     DASRBV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102836"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFA02_01525"
     CDS             complement(<1..292)
                     /locus_tag="VFA02_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08555.1"
                     /translation="MIVISWLHAPAKDIFVAQSATGSENGASCSSAYSLAWLNTSSNW
                     GTGATQVGPGDVVHLCGPITSPITFYGSGTNAAPITLYFESGASMQQPVWSTP"
     gene            complement(435..1094)
                     /locus_tag="VFA02_01530"
     CDS             complement(435..1094)
                     /locus_tag="VFA02_01530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZU08556.1"
                     /translation="MLFFDEADSIGKEQFRTLRSRLYQLREQRKLSALLISSAMAEEG
                     KSFTAANLAHVFALQRERRVVLVDCDLRRGSLASFLGTSHTPGLSEYLSGECSFEEIL
                     QTGGPGNFSLVPCGTRVQQPGELIASQRMAKLMEQLRARFDWVIVDTPPATLFSDASV
                     LADLCDGVLLVVKSGATPIRLAKNASLEFKKKSLLGVVLNKSADVLHDSSYYAYASAE
                     A"
     gene            complement(1304..2137)
                     /locus_tag="VFA02_01535"
     CDS             complement(1304..2137)
                     /locus_tag="VFA02_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU08557.1"
                     /translation="MYEKHFQLKTTPFSVSPDPKFLYPTSVIKEALATLAYGVNNRKG
                     FILLTGDVGTGKTTILNIFMQWLRSQNAATAFVYNPRLRPEELLDFMLADFHVRCPST
                     VKSRRLLRLTDWLLERHREGQPVVLVVDEAQQLSADVLEELRLLTNLETPTEKLLQIV
                     LSGQPELDTLLAHPSLRQLRQRITLRCRTGPFSEEQTKEYIEQRLRVAGAERIDIFSS
                     EAVASIHELSGGIARIINVLCEQALIHGFCENKTTIGPEAIEAIAREYNLTGTEQLSH
                     A"
     gene            complement(2183..3625)
                     /locus_tag="VFA02_01540"
     CDS             complement(2183..3625)
                     /locus_tag="VFA02_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZU08558.1"
                     /translation="MEEQGAQFRLQDLLAILKRRLWWIVIPVLLGPVIGLLVSFKIKP
                     VYTSQAFVLVEQQKVPDTIVSPVVTDQLEARLLTMQDQILSRSNLEPLITHFDLYHKD
                     RSRVPMETLVARLRKDIKVTPLRPDGTSDLRGFYIDVSADKPQTAQEVCSEILSMFMA
                     ENLKVRAQRAEDTTTFLAGQLQDAKSKLDDYDAKLAAFKSKYLGQLPTDEDRNLQMLS
                     TMSSQLDAVNQALAQTLQQKAAQEALIAQAVNTLQASQKGADSPVDLEKELSDLRMQL
                     ATLEARYTPEYPDVVRTKAQIQTLTQQLALARKNSGNDAKAASIPDTPELAQMRVALR
                     GIDEAVRLKRAEQARLEGQIRSFQSRVQLSPVVEEQYKSLTRDYESALQFYNQLLSKK
                     TQAEMATDLELRREGEQFRVMDSPDLPASPSSPNRLKCALAGFAAGIFLGACFAVMFE
                     IKEQFIRTEDDVTKHLDLITLIGIPDFAEK"
     gene            complement(3635..5071)
                     /locus_tag="VFA02_01545"
     CDS             complement(3635..5071)
                     /locus_tag="VFA02_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08559.1"
                     /translation="MKYFLRKFSFLLWMFLIATALIAQEQQQQQPAADPDDGSGVVLQ
                     SANDGPDATDGDVPTVTADERPLTGGRVIGTGSRGPRHSFFVPGFRVAQILDSNAEMV
                     ADSHYRGFSSLNGDLQLIHYFGSSTSIRYAGAVRYDTRAEVEGLQQFTNAHALSVSRL
                     DRFRSWDLLFDNETQYSQGSNFGATGMEGLGSVITATSTWGGVGNIQLESTALQPDLA
                     PNQSILTLRSGRVSNTVLGEADYRPDDKYTFTMTGFYGLLHFTDKGLIDTSQEGFVAG
                     ANRALSPTDSIAFEYGFSNFNFSGTTESLKNNYIGILYGRKLTGQIAVSAGAGPQFIS
                     DRTSISNDDRLGWQGRATLDWQMRRVGLHAAVLRNLTSGSGVLFGAYTNSIQGGMGYG
                     ISRNWDSSFIFGWARNTAVTSALRYNTQYFGASAGRHVGRDRSIFFSYDLQHQTSFGC
                     ASTTCAVNGVRHVFGVGFSWTHQPIGMF"
     gene            complement(5081..5689)
                     /locus_tag="VFA02_01550"
     CDS             complement(5081..5689)
                     /locus_tag="VFA02_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU08560.1"
                     /translation="MKLYGALLLSTLVPASAQTFVNKGAPDVQAGAHKPVVNDATDPA
                     YVIGADDVLAVTVWKEPELSATVPVRSDGKISLPLLNDVQAAGLTPAQLAADVSEKVR
                     KFITDPSVTVIVTQMNSRKAYIMGLVNHQGMVRLQANMTVLQALSAAGGLAPFANRKK
                     IYVLRTENGQEEKLPFNYDAVIKGKDSAQNVLLKPGDTVVVP"
     gene            complement(6179..>6547)
                     /locus_tag="VFA02_01555"
     CDS             complement(6179..>6547)
                     /locus_tag="VFA02_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08561.1"
                     /translation="EKADRNNIDFSRYMKQATVEGSKRVCWNSWIAPHNFEGFFMNMG
                     DMLVKAGDWQTAQKIYAQAKLSPTYDQWKYSEVLEGRIRDAQTNVANFNSNDPKLDKL
                     HQRIMIATPIACMACHENGN"
BASE COUNT         1498 a         1824 c         1616 g         1609 t
ORIGIN      
        1 ctggcgtact ccatacgggt tgctgcatgg aagcgccgct ctcaaaataa agcgtgattg
       61 gggccgcatt tgtacccgat ccataaaacg tgatcggaga agtaatgggc ccgcacagat
      121 gcaccacatc cccagggcca acctgggttg cacccgtgcc ccagtttgaa gacgtattca
      181 gccacgcaag cgaatatgcg ctggagcagc ttgccccatt ttcagatcca gttgcactct
      241 gcgcaacaaa gatgtccttt gccggagcat gaagccagct tatgacaatc atgatcagga
      301 caaacgcgac atttcggcat tttttaagtc gcgtgttacc agatgcggca ttcgcgacgc
      361 ctcgccttct gagcaaaaga cctcccaaca aaagatcaac cccggatgag cataaattgc
      421 acgtaaacac ctggctatgc ttctgcactt gcataggcat aataactgga gtcgtgaagc
      481 acatcggccg atttgttgag caccacccca agcaggctct ttttcttgaa ttccaggctt
      541 gcattttttg caaggcgaat cggtgttgct ccggatttca caaccagcag tacaccatcg
      601 cataaatccg caagtacgct tgcgtcggaa aaaagtgtgg ctggcggagt atctacaatc
      661 acccagtcaa aacgcgcgcg taattgctcc atcagcttgg ccattcgctg gctggcaatc
      721 agttccccgg gttgttggac acgtgttccg catggcacaa gggaaaaatt gcctggaccg
      781 ccggtttgaa gaatttcttc aaaagagcac tccccgctca gatactcaga gagtcctggg
      841 gtgtgcgaag tacccagaaa tgaagccagg gaaccacggc gtaaatcgca gtctacaagc
      901 acaacgcgtc gttcacgctg taatgcaaac acatgggcca gatttgcagc cgtaaagctt
      961 tttccttctt ccgccattgc gctggaaata agcaaagccg agagcttgcg ctgctcgcgc
     1021 aattgataga ggcgtgagcg taaggtccgg aactgctcct tgccgatgga gtcggcttca
     1081 tcaaaaaaaa gcatttttct tttctctggg gaccacacct tctgtgctga aagagcgggg
     1141 aaaggagcac ggtccagttc ttccctgatt ggcggcgcct cccctctggg acgaaccatc
     1201 tcttcaatca cgaaaacacc tgctgcttgt ccgttcctgt gttctttttg cgccttctgc
     1261 aatgcttcgt atatgcggct catgataatt ccttttcccg cgattatgcg tgggacagtt
     1321 gttctgttcc ggtcaggttg tactcccggg ctatggcctc aatggcttcc ggaccgattg
     1381 tggttttgtt ctcgcagaac ccatgaatca gcgcttgttc gcacagcacg ttgataatgc
     1441 gcgcaatacc cccagaaagc tcgtggatgc tcgcaacggc ctcggaagaa aagatgtcta
     1501 tacgttctgc tccggcaact ctcagccgct gctcgatata ttccttcgtc tgctcttcgg
     1561 aaaacggtcc ggtgcggcag cgcaaagtaa tccgttggcg cagttgccgc aatgaaggat
     1621 gcgcgagcag cgtatccagc tctggctgtc cagagagcac aatctgcagc agcttttccg
     1681 tcggcgtctc caggttcgtc aacaggcgaa gctcttcaag aacatcggcg gaaagctgct
     1741 gcgcctcgtc caccacgagc accacaggct gcccttcccg gtgccgctcc agcaaccagt
     1801 cagtaaggcg aagcagacgg cggctcttca cggttgaagg gcagcgtaca tggaagtcag
     1861 ccagcataaa gtcgagaagc tcttccggcc gaaggcgcgg attgtagaca aaggctgttg
     1921 ccgcattctg agaacggagc cattgcataa agatattgag aatggtcgtt ttgccggtcc
     1981 ctacatcccc cgtaagcaga atgaagccct tgcggttgtt tacgccatac gccagcgtcg
     2041 ccagcgcttc tttgatcaca ctggtgggat aaaggaactt cggatctgga cttacgctga
     2101 aaggcgtggt cttcaactgg aagtgtttct catacatggt tctgctgttc cgcctttcct
     2161 gctctgaaca aattctgcat gactactttt ccgcgaagtc agggatgcca atcagcgtga
     2221 tcaggtccag gtgcttcgtc acatcatctt ctgtccggat aaactgctct ttgatctcaa
     2281 acatcaccgc aaaacaggcg cccaggaaaa ttcccgcggc aaatccggcc agcgcgcact
     2341 ttaggcggtt gggagaactt gggctggcag gtaaatccgg cgaatccata acacggaact
     2401 gttccccttc tctgcgcagt tccaggtccg tggccatttc tgcctgggtt ttcttgctga
     2461 gcagttggtt gtagaactgc aatgcgcttt cgtaatcccg ggtaagcgat ttgtactgtt
     2521 cttctaccac tgggcttaac tgcacgcgcg attgaaagct ccgtatctgg ccttccagcc
     2581 gcgcctgctc tgctcgcttt aagcgcacag cttcgtctat tccgcggagt gctacccgca
     2641 tttgtgccag ttccggtgtg tctggaatac ttgcagcttt cgcatcgttg ccggaatttt
     2701 ttctggccag cgccaactgc tgggtcaaag tttgtatctg cgctttggtc cttacgacgt
     2761 caggatattc aggcgtgtag cgggcttcga gtgttgccag ttgcatcctc agatcactca
     2821 gttccttctc caggtcaacc gggctgtcgg cccccttttg ggatgcctgc aaagtattga
     2881 ctgcctgcgc gatcagcgcc tcctgcgcgg ccttctgctg caaggtctgc gccagcgcct
     2941 ggttcacggc atcaagctgc gagctcatcg tggaaagcat ctgcagattg cgatcttcat
     3001 ccgttggcaa ttggcctaga tatttgcttt taaatgctgc aagctttgca tcatagtcat
     3061 ccagtttgct ctttgcatcc tgcaattgtc cggcaagaaa ggttgttgtg tcctctgctc
     3121 gctgtgcccg gactttcagg ttttccgcca tgaacatcga aagtatctcg gagcagacct
     3181 cttgcgcggt ttggggctta tctgcgctta catcaatgta gaacccgcgc agatcgctcg
     3241 ttccgtctgg ccggaggggt gtgactttga tgtctttacg taaacgcgct accagagttt
     3301 ccatcggcac acgcgacctg tctttgtgat atagatcaaa atgcgtaata agcggttcca
     3361 gattcgagcg gcttagtatc tggtcctgca tcgtcagcaa ccgcgcttcg agctgatctg
     3421 taactacagg acttacaatt gtgtccggga ccttctgctg ctcgaccagt acaaatgcct
     3481 gcgacgtgta aaccggcttg atcttaaacg agaccagcag tccaatgact ggcccgagca
     3541 ggaccggaat tacgatccac catagccgcc ttttcagaat ggccagaagg tcctgaagtc
     3601 tgaattgtgc gccttgttct tccatttctt gtcctcaaaa catcccaata ggctgatggg
     3661 tccacgagaa acctacgccg aatacgtgac gaaccccatt gacggcgcag gttgttgaag
     3721 cgcaaccaaa tgaagtttgg tgttgcaggt catagctgaa gaaaatgctc cggtcgcggc
     3781 ctacgtggcg ccctgcagaa gccccgaaat attgcgtgtt atagcgcagc gcagaagtta
     3841 cagccgtatt gcgtgcccag ccaaaaataa aagacgaatc ccagttgcgt gaaatcccat
     3901 agcccatacc gccctgaatg ctattggtat acgcgccaaa gagcacgcct gatccggaag
     3961 taagattgcg tagcacagca gcatgaagtc cgacgcgcct catttgccag tccagtgttg
     4021 cgcgtccctg ccacccaagc cggtcatcat tactgataga cgtgcggtct gaaataaatt
     4081 gagggcctgc gccggcagaa acagcaatct gacccgtcag cttccggcca tacaaaattc
     4141 cgatgtaatt gttctttaac gactcagtag tgccagagaa gttgaaatta gaaaagccgt
     4201 attcaaaagc gattgaatcc gtaggagata gggcgcggtt tgctccggca acaaatcctt
     4261 cctgagaagt atcaatcaag cctttgtctg taaaatgcag cagcccgtaa aaccccgtca
     4321 tggtaaatgt gtacttatca tccgggcgat aatccgcttc tcccagaacg gtattgctca
     4381 ccctgccgga acggagagta agaatactct gattaggcgc aaggtcaggc tgcaatgcgg
     4441 ttgattcaag ctgaatattt ccaacgcctc cccaggtgct tgtggccgta atcacagatc
     4501 cgagcccttc cataccggta gcgccaaaat ttgacccttg tgagtactgc gtttcgttat
     4561 caaacagaag gtcccaggag cggaacctgt ccagacggga aacagaaaga gcatgtgcat
     4621 ttgtgaactg ctgaagtcct tctacctctg cccgcgtatc gtaccgcaca gcgcctgcat
     4681 agcgaatgct ggtgctgctg ccgaaatagt gaatcaattg caggtcgcca ttcagacttg
     4741 agaatccgcg atagtgactg tcagccacca tctcggcatt ggaatccaga atctgtgcaa
     4801 cccggaatcc agggacaaag aagctgtgcc gcggcccacg tgatcctgtt ccaatgacac
     4861 gaccccctgt cagcggtcgc tcgtccgcag tgaccgttgg aacatcacca tccgtcgcat
     4921 ccgggccatc atttgccgat tgcaggacca caccactgcc atcatccgga tccgcagcag
     4981 gctgttgctg ctgttgctcc tgcgctatta aagcggtcgc aatcaaaaac atccaaagca
     5041 aaaaagaaaa cttacgcaaa aaatatttca taggctcgcc tcatggaact acgacggtgt
     5101 cccccggctt cagaagcaca ttctgcgcag agtctttgcc ttttatcacc gcatcgtagt
     5161 taaacggcag cttttcttct tgcccgttct cggtccgaag aacatagatc ttcttgcgat
     5221 tggcaaacgg ggccaacccc ccagcggcag aaagggcctg caacaccgtc atatttgcct
     5281 gcaaacgcac catgccttga tgattaacca gccccataat gtaggctttg cggctgttca
     5341 tctgggtaac aattacggtg acgctcggat ctgtgatgaa cttcctgact ttctcgctca
     5401 catcagcggc aagttgcgcc ggcgtcagcc ccgccgcctg cacatcattc agaagcggca
     5461 gagaaatctt gccatcggag cgcaccggca cagtcgcaga caattccggc tctttccata
     5521 ccgtgactgc cagaacatca tctgcgccaa ttacataggc aggatctgtc gcgtcattta
     5581 ccaccggctt atgcgctcct gcctgtacat ccggcgcccc tttattcaca aatgtctgcg
     5641 ctgacgccgg gaccagcgtt gacaacaaga gcgcaccata aagtttcatc cttccaacct
     5701 ccctgacggg cttaggcctc cgcgactgtt atctagcacg gcgcgtgcca tagtccgccg
     5761 tagccggaag gttaggactc acacaacgag ggggcatttt tctgaagata tgtattggag
     5821 taaggtctgc tggaaaatcc tgtggtttgc agggacgcga cctgagtcct gccataaatc
     5881 gtctgaaatc aatgcggctt taatcactat ggaactgatt gttcagccat acggatcttc
     5941 cttgtaggtc tgcttcgata cacctgtttg cgccatatcc agtctgaaac aaccaaattc
     6001 ttgccggaac gaaggcaata ttctggcttc acaatggaaa tctttttcct atccatctgc
     6061 aggcctattc ctatttgata gggacgggcc ctctccggac ccgtattggg ctttatctta
     6121 ttggctcgcc atatggttag aactgctgcc ggtggaaacc atgcgtcttg cctctgcctc
     6181 aattgccatt ctcatggcag gccatgcacg caatgggtgt agcaatcata atccgctggt
     6241 gcagtttgtc cagtttcgga tcattgctgt tgaaattggc aacattggtc tgcgcgtctc
     6301 ttatgcgccc ctccaacact tcgctatatt tccattgatc atacgtaggc gaaagcttcg
     6361 cctgggcata aatcttctgt gctgtctgcc aatcgccggc cttcaccagc atatctccca
     6421 tattcatgaa gaacccttca aaattatgcg gagcaatcca cgagttccag cagacgcgct
     6481 tgctgccttc cacagttgcc tgtttcatgt acctggaaaa atcaatgttg ttgcggtctg
     6541 ctttttc
//
LOCUS       DASRBV010000035        12388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_712093, whole genome shotgun sequence.
ACCESSION   DASRBV010000035 DASRBV010000000
VERSION     DASRBV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712093"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VFA02_01560"
     CDS             <1..2052
                     /locus_tag="VFA02_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU08562.1"
                     /translation="SGPEPTRHVADVTVSKHDELDTYLPAFRATVTEAKAGSVMCAYN
                     SINGEPACANQFLLEDQLRGKWKFGGYVVSDCGAVVDIYQNHHFKPTQAQASAISLQR
                     GMDNECVDFTFKVKDNHDYKPYLDAVQQGYLKESDIDKALVRLFTARMKLGMFDPPGM
                     VPYTKINEDELNSPEHRELARKIADEAMVLLKNDGTLPLKTSGVKLAVIGPLASQTRV
                     LLGNYNGIPTHTVSILEGLRKEFAGDSIQYVAGTQFLNHNADPVPASALSGLKVSYSK
                     LDMTNINRPEAMKPLAESTVSTIDAANQPIPAEAANVHPLAIRYEGTLTAPETGDYNL
                     GLKANGFFRIQLDGKNVTSSYGGNASEAKLGRVHLEAGRPAALRVEYTPPEKGKPAAQ
                     LVWSKVDLRPQPEAIEAAKNADVVVAVLGISSELEGEEMQVSEPGFKGGDRTSLDLPK
                     PEEDLLEALVATGKPVVLVLTNGSALSVNWAKAHVNAILDAWYPGEEGGTAVAETLSG
                     KNNPAGRLPVTFYTGVDQLPPFESYAMKGRTYRYFDGTPLWPFGYGLSYTTFSYSGLK
                     LPSSVSAGQPMTAAVTVTNTGKVAGDEVAQLYLSFPDVKGAPIRALRGFRRVHLAPGQ
                     SQTVQFELKPRDLSMVTDDGKIIVPEGKYSVSVGGGQPNTGAPGVAGAFQVKGTVSLP
                     E"
     gene            complement(2359..2433)
                     /locus_tag="VFA02_01565"
     tRNA            complement(2359..2433)
                     /locus_tag="VFA02_01565"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2399..2401),aa:Phe,seq:gaa)
     gene            complement(2549..5005)
                     /locus_tag="VFA02_01570"
     CDS             complement(2549..5005)
                     /locus_tag="VFA02_01570"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZU08563.1"
                     /translation="MTSVWYGRILPVLFFALPAFAQSWLAPIADFPLSQSDITIRQHA
                     EAQKPFTVAGECGTFVGQQDGSFEAWIFPIKLLSHFRIQAELENYPIPIDVNQQAAEI
                     EISPDHTTITYSHAAFTIREILFATPCSQNGTGIMALFQVESVRPISLTFSFTPEVKR
                     MWPAPNYNAPYPEWVKLGNSGYYILHTDSQDLAAAIAMPNAQPGILAPYQERPKFWPL
                     QFVLHVDPKRDAGKFFPLLMAVGNDEASAKAPALTIQLERLNQQAQQLYAQTATYYAH
                     FFDKRLIADTPDKQFNDALRWAELSIDQLRVRHGDEVGLVAGFYSSGDSNRPGFGWFF
                     GRDTLYTLYAVNSYGDFALTREALEFLIKRQRSDGKIMHEYSQTADMVDWAKFPYEYA
                     AADATPLFIMAIEDYVNASGDTAFLRQHWDTVEKAWQFERTHDTDGDGIYDNAQGTGW
                     VESWPNGLPHQEIYLAALDQQASGAFSRLAKQMNKPDESSEAAARAQKIAGTIEQEYA
                     GPMYAFSRNADGTLDKAATVYPTVAWWDGHYALKQPDAMFERWASDEFSTDWGLRDVS
                     DREPFYDPISYHQGSVWPLFTGWTSLAEYRTGHTLSGYAHLMQNADLTWAQDLGAVTE
                     LLSGAFFQPLGRSTTHQLWSSAMVITPAIRGLFGLTVDAAHNTMTVAPHLPADWDHAT
                     LRHISVGTNLTDLEFQREGNTLLVRASNSNVKLSQTRIPLPPVEAGIPHELPLPGAET
                     QQLKILNQKEDAHSLMLELEAQGGSAYDLPLRLNGLRGTVHAEGATTSLPESRSGLGR
                     LHIVFPQGAGYQKQTVRITW"
     gene            5034..6572
                     /locus_tag="VFA02_01575"
     CDS             5034..6572
                     /locus_tag="VFA02_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZU08564.1"
                     /translation="MHWARRCQLVAVCLFCCSIAAAAQAVRSVHVPDAEERTARAFEE
                     ARKQGPLALRAFFYEMPKGGDLHVHLSGAVYAESFIRAAGEDGLCVDTVKLEFTRPER
                     GQCKAGEVPASDVPQNQKLYDALIDAFSMRTFVPVAGESGHDHFFNTFDKFSGTSKAH
                     TPEWIDEVASRAAAQNEQYLELMETPDFKPAAVLAAKVGFHSDFAQYREQLLAAGFRN
                     FVPAARAFFDDFETARRQREHCGQAGEQPACKVEVRYIYQVLRGLPKEAVFAQILLGF
                     EVASVDPSVVGINLVQPEDCYVCMRDYELHMQMIATLHALYPKVHITLHAGELAPGMV
                     PPDGLRFHIRSAVEVAHAERIGHGVDIMYEDRPYELLKEMAAKHVLVEINLTSNDVIL
                     NIKGEEHPFMLYRKYGVPVALSTDDEGVSRIDLTHEYVRAAVTYPLAYRDFKQMARAS
                     IEHSFLPGASLWQQSEQPVTQCRGQIGRDAPVGGCAEFVRSSKKAQQEWELERRFRVF
                     EASF"
     gene            6596..7867
                     /locus_tag="VFA02_01580"
     CDS             6596..7867
                     /locus_tag="VFA02_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU08565.1"
                     /translation="MRIVFSTFGTFGDVNPLIALALELKRRGHGPVLAVPEMFRLKIE
                     PLGIGFFPVRPDQPETDKELVAKIYDRRTGTEYGLRKFLFPVLRQSYEDLLAAVQADG
                     GADLLVTGELCYAGPIVAEKTGIRWASYVLAPFSFFSAYDPPVLPPYPALAKAQLALP
                     ALGHVTAWVARRVTRTWPEPVYALRRELGLARGKDPIFDAKHSDRLVLALFSRVFGEP
                     QPDWPAHTRVTGFVFYDGDAGRTELPSALAAFLEAGPPPLVFTLGSAAVLDAGDFYEQ
                     SARAAQMLGMRAVLLTGNDPANLPKAPLPESICVAPYAPYSRLFPRAAAIIHQGGIGT
                     TAQALRAGRPMVVMPYSHDQPDNARRVRRLGVAKTIQRRSYTAKRVARAIREVLGDSS
                     YLERASMIRKEMEQEDGVQAACDALEALLAR"
     gene            complement(8043..8867)
                     /locus_tag="VFA02_01585"
     CDS             complement(8043..8867)
                     /locus_tag="VFA02_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU08566.1"
                     /translation="MKLYRTTQGIFVEESGHFYPVPAASWDELLIRDDLHAYVRRAAQ
                     SGPELHFDPAGILAPAVSQEVWAAGVTYYRSRDARMEESRSAGGGDFYDRVYSAERPE
                     LFFKATGPRVVGPNQPVRIRSDARWSVPEPELTLFIQPTGKIVGYTIGNDMSSRDIEG
                     ENPLYLPQAKVYDGSCALGPCILLSDAPLAKTTAIQLEILRKGRTAYSGSTTLAELKR
                     EPEHLVEYLFRDNTFPQGVFLMTGTGIVPGDGFTLSSGDVVKISIDQIGLLENPVA"
     gene            9074..10594
                     /locus_tag="VFA02_01590"
     CDS             9074..10594
                     /locus_tag="VFA02_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU08567.1"
                     /translation="MTSSLHAVATAIFCACFLLVTIAGFWAARWRRADLHSLEEWGLA
                     GRRFGTLITWFLVGGDFYTAYTVIAVPAALYGTGAPGFFAIPYCILLYPYMMLVLPRL
                     WQVCHRRGYVTLADFVRGRFGHRPLAVAIALTGILATMPYIALQLVGMQEVISALGLR
                     GEWPLVAAFVILAAYTYSSGLRAPAVIAIVKDIMLYGMVVAAIVVIPAKLGGYAHVFS
                     AAEAALAQKKPPASIILQPSQYWSYATLAFGSAIAIVLYPHTATGVLSSSSGRVIKRN
                     AALLPAYNLLLALIALLGYFALAAGIHAADTSSAVPLLFVRMFPEWFSGFCLAAIGIG
                     ALVPAAIMSIAAANLFTRNLYGELIQRSMTPKQEAAQARLVSLFLKIGALAFVLFVRA
                     QYAIELQLLGGIWIAQIFPAVILGLFTRWFRGTALFWGWLAGMATGTGMAWARDLKSS
                     VYPLHFGGHTFAMYAAVPALLVNLLVSAAITLLMQALGSEHDKDATAATDYEMQEA"
     gene            10688..11209
                     /gene="fabZ"
                     /locus_tag="VFA02_01595"
     CDS             10688..11209
                     /gene="fabZ"
                     /locus_tag="VFA02_01595"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZU08568.1"
                     /translation="MENQNHIPEARQTLEITDIMAILPHRYPFLLIDRVVEIERRKRI
                     VAIKNVTINEPFFQGHFPNYPIMPGVLVVEAMAQAGGALLLTEVPDRHNKLMVFTGIE
                     RAKFRRPVVPGDQLRIEINVLQWKTRAVRMEGLVTVDGKLACEAIVMCQLVPRRKQEA
                     QSEAPVLATVTEE"
     gene            11206..11982
                     /gene="lpxA"
                     /locus_tag="VFA02_01600"
     CDS             11206..11982
                     /gene="lpxA"
                     /locus_tag="VFA02_01600"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZU08569.1"
                     /translation="MSIHPTAIVAPEAVIPDSCIIGPYCTVGPHVVLGDECELVSHVV
                     LDGHLKAGARNKFYSFACVGVAPQDLKYKGEPTGVVMGDDNIIRECVTISRGTAGGGG
                     TTHIGSHCLIMAYSHIGHDSVIGSHCILANAATLAGHVVVEDYATVGALNQVHQFCRI
                     GKYAYTGGGTTVVQDVLPFSLTSAKRETRAYGLNKVGLERRGFDRERLRALQHAYRYL
                     LAGKMNTSQALEKLKSEGVPTEDVAYLVEFIEKSERGVVK"
BASE COUNT         2490 a         3426 c         3838 g         2634 t
ORIGIN      
        1 agcggccctg agccgacgcg ccacgttgcc gatgtgaccg tcagcaagca tgacgagctg
       61 gacacctatc tgcccgcctt tcgcgccacg gtgacggaag ccaaagccgg ttcggtgatg
      121 tgcgcgtata acagcatcaa cggcgaaccg gcctgcgcca atcagttttt gctagaagac
      181 cagctgcgcg gtaagtggaa gtttggcggc tatgttgttt ccgattgcgg agcggttgtg
      241 gacatctacc agaaccacca cttcaagcca acacaagcgc aggcttcggc catcagcctg
      301 caacggggaa tggacaacga gtgtgtggac tttaccttca aggtaaaaga caatcacgat
      361 tacaagcctt atctcgatgc cgtccagcaa ggctatctga aagagagcga cattgataaa
      421 gcgctggttc gcctgtttac ggcgcggatg aagctgggca tgttcgatcc gccgggcatg
      481 gtgccgtata caaagatcaa cgaagatgaa ctgaacagcc ctgagcatcg cgagcttgcc
      541 cgcaagatcg ccgatgaagc gatggtgctg ctgaagaacg acggcacgct tccgctgaag
      601 accagcggtg taaagctggc cgttatcggg ccacttgcca gccagacgcg cgtgcttctg
      661 ggcaattaca acggaattcc cacgcacact gtttcgattc ttgaaggact gcgcaaggag
      721 ttcgcaggcg acagcattca gtatgtcgcg gggacccagt tcctgaacca caacgcagac
      781 ccggtaccgg cttcggcgct ttcgggcctg aaggtcagct actcgaaact ggacatgacc
      841 aacatcaacc gtccggaagc gatgaagccg cttgcagaaa gcactgtttc caccattgat
      901 gctgctaacc agccgattcc cgctgaggcg gccaacgtgc atccgcttgc cattcgttat
      961 gaggggacgc tgactgcccc tgaaaccgga gactacaatc tggggctgaa ggcaaacggg
     1021 ttcttccgca ttcagcttga tggcaagaac gtgacttctt cctatggcgg caatgccagt
     1081 gaggcgaaac tgggccgcgt acacctggaa gccggcaggc cggcggcgct gcgtgtggaa
     1141 tacactccgc cggagaaggg caagcccgct gcgcaactgg tctggtcgaa ggtagacctt
     1201 cgtccgcagc cggaagcgat agaagcggca aagaatgcgg acgttgtggt tgccgtgctg
     1261 ggcattagca gtgagctgga aggcgaagag atgcaggtga gcgagcctgg attcaaagga
     1321 ggcgaccgca ccagccttga tctgcccaaa ccggaggaag accttctgga ggcgttggtc
     1381 gcgaccggaa agcctgttgt tctggtgctg acgaatggca gcgcgctttc ggtgaactgg
     1441 gccaaagcgc acgtaaatgc cattctggac gcgtggtatc cgggcgaaga aggcggcacg
     1501 gcagtggctg aaacactttc cggcaagaac aatccagcag ggcgcttgcc ggtcaccttc
     1561 tataccggag tggaccaact tccaccgttc gagagctacg cgatgaaggg ccgcacgtac
     1621 cgctacttcg atgggacacc gttgtggccg ttcggttatg gccttagcta cacgacgttt
     1681 tcctatagcg ggctgaagct gccctcgagt gtgagtgcgg gccagccgat gacggccgcc
     1741 gtcactgtaa cgaacaccgg gaaggtggca ggtgatgaag tcgcgcaact gtacctcagc
     1801 ttcccggacg tgaagggcgc gcccattcgt gcgttgcgag gattccggcg cgtacatctc
     1861 gctccggggc aatcgcagac ggtgcagttt gagctgaagc cacgcgacct gagcatggtg
     1921 acggacgatg gcaagatcat cgttccagaa ggaaagtatt ctgtgtcggt gggaggcggc
     1981 cagccaaata caggagcgcc tggcgttgcc ggagcgttcc aggtgaaagg cacagtcagc
     2041 ttgccggagt agtccgatgc cccattcagg cgctcatggg ggcttgaatg gggtgtttgt
     2101 ttgcagctaa gctgttatga gcatttttga ccgtggccgc tgcagcacca cggctgcctc
     2161 gcctgcgctt gctcaggctg gatacggtat gcagagcggg ccacaatcag gcatatttcg
     2221 cgcggcctga gaacgaaaga caggagagaa ggtggtggcc agggacggga tcgaaccgcc
     2281 gacgccggcc ttttcagggc cgcgggccac aatcaggcat atttcgcgcg gcctgagaac
     2341 gaaagacagg agagaaggtg gtggccaggg acgggatcga accgccgacg ccggcctttt
     2401 cagggccgcg ctctaccact gagctacctg gccttgggat gtgtccacaa ggagcgtttc
     2461 cggatgtctg agaaaacgaa tgcagaggac gggacggccc ccagaaggca ctctgatatc
     2521 aaacaacctc acttagtata gcaaagactt accatgtgat tcgcacagtc tgcttctggt
     2581 atccggcccc ctgtgggaac acgatatgca atctgccgag gccggaacgg gattcgggga
     2641 gtgaggtggt cgcgccttcg gcgtggacgg tgccgcgcag tccgttgagg cgcaaaggga
     2701 ggtcatacgc gctgccgccc tgggcctcga gttcgagcat cagagagtgc gcgtcttctt
     2761 tctgattgag tatcttcaat tgctgtgttt ccgctccagg caggggcaat tcatgaggaa
     2821 tgcccgcttc gaccggaggc agcggaatgc gcgtctgcga taatttcaca ttgctgttgc
     2881 tggcgcggac aagcagtgtg ttgccttcac gctgaaattc gaggtcagta aggtttgtgc
     2941 ctacagaaat gtggcgcaat gtggcgtggt cccagtccgc tggcagatgg ggcgcgacgg
     3001 tcatggtgtt gtgcgcggcg tccacggtga ggccgaaaag cccgcgaatg gctggcgtga
     3061 tgaccatggc cgaagaccac aattgatggg tcgtgctgcg tcccagcggc tggaaaaacg
     3121 cgccggagag aagctcggtg actgcgccca ggtcctgcgc ccaggtgagg tctgcattct
     3181 gcatcaggtg cgcgtagccg gaaagagtgt gaccggtgcg atattcggcc agcgaggtcc
     3241 atccggtaaa gagcggccag acggagccct gatgatagct gatggggtca taaaaaggct
     3301 cgcggtcact tacgtctcgc aggccccagt cggtagaaaa ttcatccgat gcccagcgct
     3361 caaacattgc atcgggctgt ttcagcgcgt agtggccgtc ccaccaggcg acggtgggat
     3421 agacggtcgc ggccttgtcg agagtgccgt ctgcgttgcg gctgaaggca tacatcggcc
     3481 cggcgtattc ctgctcgatg gtgccggcga ttttctgtgc gcgggcggcg gcttcactgg
     3541 attcatccgg cttattcatt tgctttgcga ggcgcgaaaa tgcgccgctg gcctgctggt
     3601 cgagcgcggc gagataaatt tcctgatgcg gcaggccgtt gggccagctt tcgacccagc
     3661 cagtgccctg cgcattgtca tagatgccgt cgccgtcggt gtcgtgcgtg cgctcaaact
     3721 gccacgcctt ctcgaccgtg tcccagtgct ggcgcagaaa tgctgtgtca ccgctggcgt
     3781 ttacatagtc ttcgatggcc atgatgaaga gcggcgtggc gtccgcggcg gcgtattcat
     3841 aggggaactt tgcccagtcc accatgtctg cggtctgtga atattcgtgc atgattttgc
     3901 catcgctgcg ctggcgtttg atgaggaatt cgagagcctc gcgtgtgagg gcaaaatcgc
     3961 catagctgtt gacggcgtag agggtgtaga gggtatcgcg tccaaagaac caaccgaagc
     4021 cggggcggtt ggagtcgccg gaggaataga agccggcgac caggccaact tcatcgccgt
     4081 ggcgcacgcg gagctggtca atggaaagct cggcccagcg tagcgcgtca ttgaattgct
     4141 tatcgggagt gtcggcgatg aggcgcttgt caaagaaatg cgcgtagtag gttgcggtct
     4201 gggcatagag ttgttgcgcc tgttggttga gccgttccag ttgtatggtc agtgccggag
     4261 ctttcgcaga ggcctcatcg ttgcccacgg ccatcagcag cgggaagaat ttgccggcgt
     4321 cgcgcttcgg gtctacatgc aatacgaatt gcagaggcca gaatttcggc cgctcctgat
     4381 aaggcgcgag gatgccgggc tgcgcgttgg gcatggcgat ggcagcggcg aggtcctgcg
     4441 agtccgtatg cagaatgtaa tatccgctgt taccgagctt cacccactcc ggataaggcg
     4501 cgttgtaatt gggcgcgggc cacatacgct tcacctccgg cgtaaaggaa aaggtcagcg
     4561 agatgggccg caccgattcc acttgaaaaa gcgccatgat gcctgtgccg ttctgtgagc
     4621 atggagtggc gaagagaatt tcgcggatgg tgaaagccgc gtgcgagtag gtgatggtgg
     4681 tgtggtcagg cgagatttcg atctccgcgg cctgctggtt tacatctatg gggatgggat
     4741 agttttcaag ctccgcctgg atacgaaaat ggctgagcag cttgatggga aaaatccatg
     4801 cttcaaagct gccgtcctgc tggccaacaa aggtgccgca ttcgcctgcg acggtaaacg
     4861 gcttttgcgc ttcggcatgt tggcgaatag tgatgtcgct ctgagagagc gggaaatctg
     4921 cgatgggagc cagccaggac tgcgcaaatg caggaagcgc gaaaaataac accggcagga
     4981 tgcgtccata ccatacactt gtcatgaatt cttcctcgaa aaggaaatcg tatatgcact
     5041 gggccagacg ctgccaactc gtcgctgttt gtctgttctg ctgttccatt gctgctgccg
     5101 cgcaggcagt gcggtctgtc catgttccgg atgcagagga acggacggcg cgcgcctttg
     5161 aagaggcgcg aaagcagggg ccgctggcac tgcgcgcatt tttctacgag atgccaaagg
     5221 gaggcgatct gcatgtgcat ctgtccgggg ccgtgtatgc ggagtcgttc atccgtgctg
     5281 cgggggaaga cggcctgtgc gtggacacgg tgaagctgga attcacgcgc ccggaacggg
     5341 gacaatgcaa agcgggagaa gtccccgcga gcgatgttcc gcagaaccag aagctctatg
     5401 atgcgctgat tgatgcgttt tccatgcgca cgtttgtacc ggtagcgggc gagtccgggc
     5461 atgatcactt cttcaacacc tttgataaat tcagtgggac cagcaaggcg cacacgccag
     5521 agtggattga cgaggtggcc tcgcgcgccg cggcgcagaa cgagcagtat ctggaattga
     5581 tggagacgcc cgactttaaa cctgcggctg ttctggctgc gaaagtgggc ttccattcgg
     5641 actttgcgca ataccgtgag cagctgctgg ctgcgggctt ccgcaatttt gttccggctg
     5701 cgcgcgcctt cttcgatgat ttcgagacag cgcgccgcca gcgtgaacac tgcgggcaag
     5761 ccggcgaaca gcctgcttgc aaggtcgagg tgcgctacat ctatcaggtg ctgcgggggc
     5821 ttccgaaaga ggcagtgttt gcgcagatcc tgcttgggtt tgaggtcgcc tcagtagacc
     5881 ctagcgtggt gggcatcaat ctggtacagc cggaagactg ctacgtgtgt atgcgcgatt
     5941 acgagctaca catgcagatg attgcgacac tgcacgcgct ttatcccaaa gtacacatca
     6001 cgctgcacgc gggcgaactg gcgccaggga tggtcccgcc ggatgggctc aggtttcaca
     6061 ttcggtcggc agtagaggtg gcgcacgccg agcgcattgg gcatggcgtg gacatcatgt
     6121 atgaggacag gccctatgag ctgctgaaag agatggcggc aaagcacgtt ctggtggaga
     6181 tcaacctcac gagcaatgac gtgattctga acatcaaggg cgaagagcac cctttcatgc
     6241 tttatcgcaa atacggagtg ccggttgcgc tttccaccga tgatgaaggc gtttcgcgca
     6301 ttgacctgac acatgaatat gtgcgcgcgg cggttacata tcccctggct tatcgtgatt
     6361 tcaagcagat ggcgcgcgcc agcatcgagc acagttttct gccgggggcg agtttgtggc
     6421 aacaatcaga gcagcctgta acgcagtgcc gtggacagat tgggcgcgat gcgcctgtgg
     6481 gggggtgcgc agagtttgtc cggtcgagca aaaaggcgca gcaggagtgg gagctggaac
     6541 ggcgctttcg ggtgtttgag gcaagttttt agctgcgttg ttgacaagac ataagatgcg
     6601 cattgtcttt tctacctttg ggacttttgg agacgtgaat ccgctgattg cgctcgcgct
     6661 tgaactcaag cggcgggggc atggcccggt attggctgtg ccggagatgt ttcgcctgaa
     6721 gatcgagccg ctcggcattg gattttttcc tgtccgccct gaccagccgg agacggacaa
     6781 agagctggtt gcaaaaatct atgacagaag gacaggcacc gaatacggcc tgcggaaatt
     6841 tctgtttcct gtgttgcgcc agagctatga agacctgctc gccgccgtgc aggctgatgg
     6901 cggcgcggac ctgctggtga ccggagagct gtgctacgca gggccgattg ttgctgagaa
     6961 gaccgggatc cggtgggcct catacgtgct tgcgccgttc tcgttttttt cagcatatga
     7021 cccgccggtt ctgccgccgt atccagcgct ggcgaaggca cagcttgccc tgcctgctct
     7081 tgggcatgtg acagcgtggg tggcgcggcg cgtgacgcgc acatggccgg aaccagtcta
     7141 tgcgctgcga cgcgaattgg gtcttgctcg cggtaaagac cctattttcg atgccaagca
     7201 ctctgaccgt ctggtgctgg cgctattttc gcgcgtgttt ggagagccgc agcccgactg
     7261 gccggcccat acacgagtga cgggatttgt cttttatgac ggcgatgcgg gtaggacaga
     7321 actgccctct gcgcttgctg catttctgga agcagggccg cctccgcttg tctttacgct
     7381 gggatcggcg gccgttctgg acgcgggcga tttctatgag cagagcgcgc gtgccgccca
     7441 gatgctggga atgcgcgccg tcctgcttac gggcaacgat ccggcgaatc ttcccaaagc
     7501 gccgctgccg gagagcatct gtgttgcacc gtacgcacct tattccaggc tcttcccgcg
     7561 tgcggccgcc attatccatc agggcggtat cggcacgaca gcgcaggcgt tacgtgcggg
     7621 caggcccatg gtggtgatgc cgtacagcca tgaccagcca gacaatgcgc ggcgcgtccg
     7681 ccggctgggt gtggctaaga ccattcagcg caggagttac acggcaaaac gtgtggcccg
     7741 ggcgattcgc gaggtgctcg gtgattccag ttatctcgaa cgcgccagca tgatccggaa
     7801 ggaaatggag caggaagatg gagtgcaggc agcatgcgat gctctcgaag cattgctggc
     7861 gcggtgacat gcctttcgtg catttttcgt ttcactgcga tgcacgaaaa ggatgatggc
     7921 cattccggac ggcgcggaaa atcctggcag gattggctgg ccctctcaca tcgctacgat
     7981 tcttctgtct cctgctttgc cagcagtccc cgaggcgaaa agatccaatc cctgtgctcg
     8041 cctcatgcta ccgggttttc cagcaggcca atctggtcaa tactgatctt cactacatcg
     8101 ccactggaca aggtaaaacc gtcgccggga acaatgcctg tgcctgtcat gagaaaaaca
     8161 ccttgtggga atgtgttgtc ccggaaaaga tactccacca gatgttccgg ttctcgtttc
     8221 agttccgcga gcgttgtact gcccgaataa gcggtccgcc cctttcgcag aatttcaagc
     8281 tggatggcag tcgtttttgc cagcggagcg tctgacagca gaatgcacgg cccgagtgcg
     8341 cagcttccgt catagacctt tgcctggggt agatagagcg gattctcacc ctcaatatcg
     8401 cgcgagctca tgtcgttgcc aatcgtataa cccacaatct tgcccgtggg ctggatgaac
     8461 agagtcagct caggttcggg cacagaccag cgggcatcgg aacgaatgcg tacaggctgg
     8521 tttgggccca ccacacgtgg gcctgttgcc ttgaagaaaa gctccggacg ctcggcagag
     8581 taaacacgat cgtaaaagtc gccgcccccc gcgctcctgg attcttccat gcgggcatca
     8641 cggctgcgat agtaggtgac accggcggcc cagacctcct ggcttactgc cggagcaaga
     8701 atccctgcag gatcaaagtg aagctccgga ccgctctgtg ccgcgcggcg cacgtatgcg
     8761 tgcagatcat ccctgatgag gagctcgtcc cagctagcag caggtacagg atagaaatgg
     8821 ccactttctt ccacaaagat gccttgtgtt gtccggtaga gcttcaagtg tctcctcctt
     8881 atgcccgagt gcgcatatgt agttgtatcg cggactgtgt gcagagtcac gctcggtttg
     8941 gtccgccgtt tggtctggag aaagcaattc cttcgtcctc tctgcacgcg gggtaagggt
     9001 aacaatgact ggaaataaaa agccccgctt cttgatgtag ggatggtcat tgtttaccct
     9061 caaagacacc tgaatgacat cctcactgca cgctgttgcc actgcaatct tctgcgcctg
     9121 ctttctgctg gtgaccatcg ccggtttctg ggcagcgcgc tggagacgcg ccgacctgca
     9181 cagtctggag gaatgggggc ttgccggacg ccgctttggc acgttgatta catggtttct
     9241 ggttggcggt gacttctaca ccgcatacac ggttatcgct gttccggcgg cgctttatgg
     9301 aacaggtgct ccgggcttct tcgccattcc ctactgcatc ctgctttatc cctacatgat
     9361 gctggtgctg ccgcgattgt ggcaggtgtg tcatcgccgc gggtacgtca cgctggcaga
     9421 cttcgtccgc ggcaggttcg gccatcggcc cctggccgtt gccattgcac tcaccggaat
     9481 tctggcgacg atgccttaca ttgccctgca gcttgtaggc atgcaggagg tgatttctgc
     9541 cctcggactc agaggcgaat ggccccttgt ggctgcattt gtgattctag cggcctacac
     9601 ctactcaagc ggactgcgcg ctcctgccgt catcgccatt gtcaaggaca tcatgctcta
     9661 tggaatggtg gttgccgcga ttgttgtcat tccggcaaag cttggcggat atgcgcatgt
     9721 gttttctgcg gccgaggcgg cccttgcaca gaagaagccg cctgcttcca tcatcctgca
     9781 gccttcgcaa tactggagct atgccacgct cgccttcggc tcggccattg ccattgtgct
     9841 ctatccgcat accgccacag gggtgctcag ctcatccagc ggacgcgtca tcaagcgcaa
     9901 tgctgcattg ttgcctgcct ataatctgct gctggccctg attgctctgc ttggatactt
     9961 cgcacttgct gcgggaatcc atgcagcaga cacttcaagc gctgtgccgc tgctctttgt
    10021 ccggatgttc ccagaatggt tttcaggctt ctgccttgca gccatcggaa ttggagcgct
    10081 ggtcccggcg gcgattatgt ccattgctgc ggcaaacctg ttcacgcgca atctgtatgg
    10141 cgagctgatc cagcgctcca tgactccaaa acaggaggcg gcgcaggccc ggctggtctc
    10201 gcttttcctg aagattggcg cgctggcatt tgtgctcttt gtgcgcgcgc agtatgcaat
    10261 tgaactgcaa ctgctcggcg gcatctggat tgcccagatc tttcccgccg tgattctcgg
    10321 cctgtttacc cgctggttcc gcggcaccgc cctcttctgg ggatggcttg caggcatggc
    10381 aacaggcact ggcatggcct gggcgcgcga cctgaaaagc tcggtctacc cgctgcactt
    10441 tggcggacac accttcgcta tgtacgctgc ggtcccggcg ctgctggtca atctgctggt
    10501 ctccgccgcg attacgctgc tgatgcaggc actgggcagc gagcacgaca aagacgcgac
    10561 tgccgcaacg gactacgaga tgcaggaagc gtagggtgca tgccatttct tcatcctgct
    10621 ttcctttact gaaaagggaa caggatgaga cgggtccagc gggaaagtat aatgacctct
    10681 ggcagatatg gaaaatcaga accacatacc ggaggcgcgc cagacgctgg aaatcaccga
    10741 catcatggcg atcctgcccc atcgctaccc ttttcttctg attgatcgcg ttgttgaaat
    10801 tgagcgcaga aaacgcattg tcgccatcaa gaacgtaacc atcaacgagc cgttctttca
    10861 gggacacttt ccgaattacc ccatcatgcc tggcgtactg gtcgtggaag ccatggcgca
    10921 ggccggcggc gcgctgctgc tcaccgaggt ccctgaccgc cacaataagc tgatggtctt
    10981 taccggcatc gagcgcgcga agtttcgccg tcctgtcgtg cccggcgacc agctccggat
    11041 tgaaatcaac gtgctgcaat ggaagacgcg cgccgtgcgc atggaagggc tggtcacagt
    11101 ggatggaaag ctcgcctgcg aggccattgt gatgtgccag cttgttccgc ggcgcaaaca
    11161 agaggcacaa tcggaagccc cagtgctcgc gacggtgacc gaagagtgag catccatcct
    11221 actgccatcg tcgctccaga ggcggtcatt ccggactcgt gcatcattgg tccgtattgc
    11281 acagtcgggc cccatgtggt gctcggcgac gagtgtgagc tggtctcgca tgtcgtgctc
    11341 gacgggcacc tgaaggcagg cgcacggaac aagttctatt cctttgcctg tgtaggcgtg
    11401 gccccgcagg acctgaagta taaaggcgaa cccaccggcg tggtgatggg agatgacaac
    11461 atcatccgcg agtgcgtgac catttcccgc ggaacagcag gcggcggggg aacgacccac
    11521 atcggcagcc actgcctcat catggcctat tcgcatatcg gacacgacag cgtgatcggc
    11581 agccactgca ttctggcaaa cgccgccact ctggccggac acgtggttgt cgaagactac
    11641 gcgacggttg gcgcgctgaa ccaggtacac cagttctgcc gcatcggcaa atatgcgtat
    11701 acgggcggcg gaacgacggt cgtgcaggac gttctgccct tttctctgac atctgccaag
    11761 cgggaaaccc gcgcctatgg cctgaacaaa gtcggtcttg agcgccgcgg gttcgatcgc
    11821 gagcggctgc gggcgcttca gcatgcctac cggtatctgc tggcagggaa gatgaacacc
    11881 tcgcaggccc tggaaaagct gaagagtgaa ggtgtgccta cggaggatgt ggcctacctg
    11941 gtcgaattta ttgaaaaatc tgagcgcgga gtggtgaagt aagcactccg ccggaagccc
    12001 ctcgattctt ctgcgctgac ttcgcgccct cactgcggtc aagctttggg ccgttcgttt
    12061 tgataaacca agggcgatag catttcccgt atatgatatg gcccgtcatt ctgacgaccg
    12121 cagggagaaa ggacctcagc gatgcgcgag tccagccgtt actactcaaa attttccgcc
    12181 cacgcctgca atgacgatca tcctattcgt ggacagcagt gaaacgaagc actaaaccac
    12241 ttcttctccc agtgcaaatt tcagttcgtg ggactcgttc caggccatac gcagacctcc
    12301 ggcaaccggg cggctgccca tgagccgcac ggctttgatg cccatgctca gatagaacgg
    12361 cgcctgtcca gtgggccaca gttcgcgc
//
LOCUS       DASRBV010000036         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_406790, whole genome shotgun sequence.
ACCESSION   DASRBV010000036 DASRBV010000000
VERSION     DASRBV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406790"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFA02_01605"
     CDS             <1..580
                     /locus_tag="VFA02_01605"
                     /inference="COORDINATES: protein motif:HMM:NF036579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HZU08570.1"
                     /translation="ANVLAVWQDVAPRDEQQKILERVLASRENQSVQLDGSTVPPMSA
                     MSYYFRFYLARALEHAGLAGRYLDELTPWRGMLKLGLSTWAEQPEPTRSDCHAWSASP
                     NYDLLTVVAGIHPASPGFKTVRIEPHLGKLTHLDATMPHAGGEIRVGYQKQGNEWTAR
                     VELPSGLTGEFVWNGKSHPLHAGKQILRVGER"
     gene            complement(653..2608)
                     /locus_tag="VFA02_01610"
     CDS             complement(653..2608)
                     /locus_tag="VFA02_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZU08571.1"
                     /translation="MHRLQSKGWQRICFLLIFFFTLPIFAQQKPLIDITGYTIDAQLS
                     PTDHKLAATARITFTALDNSDNAIFELHGALKVEKVTDTSGTALTGERGQNATVIVTP
                     RSPLVKGQQYTWVFTYDGVLNDETGSPVEGLKLAHVGDPISYLLYPGRWFPMTGFLTD
                     RFTADIHVKVPTGYRVIGSGATTSASGEYDFHWAKSGFPGTIIAGKFSAPVSAAPNIH
                     IYTTDAHKQFAQDYGQAALREFAYFTSTFGVPDSTQLNVVELPDDTVHVWWAPEIAAV
                     AGSYIGSNINSYRLLANTIAHQWWGNQISPATLNDEWLENGMSRYAELMYLEDQAGRN
                     AMNAAIQDVSASALAYDTIPLTSAGRLAPFSPQFQSMTLDKGGMVFHMLRWEVGQDSF
                     LKILRAVLTQYKDQPVKTSDFEKLAEAQSQQQLTPFFAQWLDGTGAPQFTNKYAVYRL
                     GNNKGFRTIGQIQQDLDLFNMPVELRIETDGKTEERRVDVVGSDSQFVVDTFGRPRRI
                     TIDPDNWILKNSTDMQVRVHILKGQNLVAAGDLTGALAEYQQALKVNPNSSLASYRIG
                     EVLFTMRNYQASINAYRDALRGDDDPKWTEVWSHIQIGKIFDVTGQRDRAIPEYRQAL
                     QTNDNTGGALNEARKYLQTPFKRADNE"
     gene            complement(2728..>3534)
                     /gene="lpxB"
                     /locus_tag="VFA02_01615"
     CDS             complement(2728..>3534)
                     /gene="lpxB"
                     /locus_tag="VFA02_01615"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZU08572.1"
                     /translation="VIYFVSPQLWAWKKYRIRKVQQYVDRMLVIFPFEESFYKQHGVD
                     ARFVGHPLAEVPLPSISRNAFAAQHGLDPSKKWTGLLPGSRFKEIRLNLPEMIEAARQ
                     LDGAYEYLLPLAPTLTPEHVTHVRSMLPETSPRVTITHDARAVLYHSQASIVASGTAT
                     VEAALIGNPFIVVYRVSGLTYAVAKRVVKVPHVAMVNLIAGREIVPELIQDDFTAANT
                     VSHLRPLLEDEAARNKMKQDLAAVAQALRTEKSPIEQVARITIELASSAR"
BASE COUNT          709 a          905 c         1129 g          791 t
ORIGIN      
        1 ggcgaatgtt cttgctgtgt ggcaggacgt ggccccacgc gatgagcagc agaagatcct
       61 ggaacgcgtg ctggcttccc gggaaaacca gagcgtgcag ctcgatggaa gcacggttcc
      121 gccgatgtcg gcgatgagtt actacttccg gttctacctg gcacgggcgc tcgaacacgc
      181 tgggctggcc ggccgttatc tggatgaact gacgccgtgg cgcgggatgc tgaagctagg
      241 cctgagcaca tgggccgagc agccggaacc gacacgctcg gactgccatg cgtggagcgc
      301 cagtccgaat tacgacctgc ttacggtggt tgcgggcatt catcctgcgt cgcccggatt
      361 taagaccgtg cgcatagaac cgcatctggg taagctgacg catctggatg cgacgatgcc
      421 tcatgctggt ggtgagattc gtgtgggata tcaaaagcag ggtaatgagt ggactgcacg
      481 ggtggaattg ccttccgggc tgacggggga atttgtatgg aacgggaaga gccatccgtt
      541 acatgccggg aagcaaatat tgcgggtggg tgagaggtga tctcccaccc ttgcctggca
      601 aagacaggac accctggttc ctgcaaagcc cctttgctaa cctcgctccg ttctactcat
      661 tgtcggcgcg tttgaagggc gtttgcaggt actttcttgc ttcgttgagt gcgccgccgg
      721 tgttgtcgtt ggtctgcaat gcctggcggt attcggggat ggcgcggtcg cgctgtccgg
      781 tcacgtcaaa gattttgccg atctgaatat ggctccagac ctcagtccat ttcgggtcat
      841 catcaccacg cagagcatcg cggtatgcgt tgatggaagc ctgatagttc cgcatggtaa
      901 aaagcacttc gccgatgcgg tagctggcca gcgagctgtt cggattgact ttgagcgcct
      961 gctgatactc ggccagtgcc ccggtcaggt ctccggcggc aaccaggttc tggcctttca
     1021 gaatatggac gcgcacctgc atgtcggtcg agttcttcag tatccaattg tctggatcaa
     1081 tggtgatgcg gcggggacgt ccgaaggtgt ccacaacgaa ctgtgaatcg gagccgacga
     1141 catccacgcg gcgctcttcg gttttgccat cggtttcgat gcgcagctca acgggcatgt
     1201 tgaagagatc gaggtcctgc tgaatctggc cgatggtgcg gaagccttta ttgtttccga
     1261 ggcggtaaac ggcatatttg ttggtaaatt gcggagcacc agtgccgtcc agccattgcg
     1321 cgaagaacgg agtaagctgc tgctgagact gggcctcggc cagcttctca aaatcactgg
     1381 ttttcaccgg ctggtctttg tactgcgtta acacagcgcg caggattttc agaaagctgt
     1441 cctggcccac ttcccaccgg agcatgtgga agaccatgcc gcctttgtcg agcgtcatgg
     1501 actggaattg cggcgagaaa ggagcgaggc gtccggcgct ggtaagcggg atggtgtcat
     1561 aggcgagcgc gctggcggag acgtcctgaa tggccgcatt catggcgttg cgccccgcct
     1621 ggtcttcgag gtacatcagc tcggcatagc gcgacatgcc attttcaagc cattcatcgt
     1681 tgagtgtggc ggggcttatc tgatttcccc accactggtg cgcgatcgtg ttcgccagca
     1741 gacgatagct gttgatgttg ctgccgatgt acgagccggc gacggcagca atttccggcg
     1801 cccaccagac gtggacggtg tcatcgggga gttccactac attcaattgt gtcgagtcag
     1861 gcacgccaaa ggtgctggta aagtaggcaa attcgcggag ggcggcctga ccgtagtcct
     1921 gagcaaattg tttgtgtgca tcggtcgtat agatgtggat gttgggcgcg gcggagaccg
     1981 gggcagaaaa ctttcctgcg atgatggtcc ccggaaaacc agactttgcc cagtggaaat
     2041 cgtattcgcc gctggcggat gttgtcgcgc cgctgccgat gacgcgatat ccagtaggga
     2101 ctttcacgtg gatgtccgcc gtaaagcggt cggtgaggaa cccggtcatg ggaaaccagc
     2161 gcccggggta aagcagatag ctgatggggt cgccgacgtg ggccagcttc agtccttcga
     2221 ccggactacc ggtttcatcg ttaagcactc cgtcataggt aaagacccag gtgtattgct
     2281 gtcctttgac gagcggtgag cgtggcgtga cgatgacagt ggcgttctgg ccgcgctcgc
     2341 cggtcagcgc cgtgcctgaa gtgtctgtga ccttctcgac tttaagggcg ccgtgtagtt
     2401 caaaaatggc gttgtcggag ttgtcgagtg cggtgaaggt gatgcgggcc gtagctgcca
     2461 gcttgtggtc ggtgggagaa agctgcgcgt caatggtata tccggtgatg tcaatgagcg
     2521 gcttttgctg ggcaaagatc gggagcgtga agaaaaaaat gagaaggaag caaattctct
     2581 gccacccttt gctttgcaaa cgatgcacgc ggagtcgtgc aaaacccaca tctctgcgag
     2641 atgttgggca cccggttgtc cggagaggcc gggtaaaaaa gcgcgaaaaa aagccggggc
     2701 cggggcagta atcagataat ggtgtcttca tcgtgctgaa ctcgccaact ctattgtgat
     2761 gcgcgccact tgctcaattg gacttttttc tgtgcgcaat gcttgtgcta cagcggccag
     2821 gtcctgcttc attttgtttc gcgcggcctc atcttccagc agaggccgca gatgtgagac
     2881 ggtattcgct gcggtaaagt cgtcctgaat aagctcaggg acaatttcgc gtccggcaat
     2941 caaattgacc atggccacat gcgggacctt cacgacccgc tttgcaacag catacgtcaa
     3001 tccggagacg cggtagacca caatgaacgg attaccaatc agggcggcct cgaccgttgc
     3061 tgtgccgctg gctacgatgc tggcctggga atggtaaagg acggcgcgcg catcatgcgt
     3121 aattgtgaca cgcggggagg tttcgggcag catcgagcgg acatgggtga catgttccgg
     3181 agtgagggtg ggcgcaagcg gaagcagata ttcgtaagcg ccatcgagct gtcttgcggc
     3241 ttcgatcatc tccgggaggt tgagacggat ctccttgaag cggctgcccg gaagcagccc
     3301 tgtccacttt ttggacgggt cgagaccgtg ttgggcagca aaggcattgc gtgagatgga
     3361 aggcagtggg acttccgcca gcgggtggcc tacaaatcgg gcatccactc cgtgctgttt
     3421 gtagaaactt tcctcaaagg gaaagatgac cagcatccgg tctacgtatt gctgcacctt
     3481 gcggatgcgg tactttttcc aggcccagag ctgcgggctt acgaagtaga tgac
//
LOCUS       DASRBV010000037         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_205223, whole genome shotgun sequence.
ACCESSION   DASRBV010000037 DASRBV010000000
VERSION     DASRBV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205223"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /gene="purL"
                     /locus_tag="VFA02_01620"
     CDS             <1..1845
                     /gene="purL"
                     /locus_tag="VFA02_01620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZU08573.1"
                     /translation="VVHGIAGYGNCFGVPNLGGETRFEDCYSGNPLVNAFALGLVRRD
                     EIFYARATGVGNPVIYVGAKTGRDGIHGATMASEEFKEGSEQKRPNVQVGDPFMEKLL
                     LEACLEAMKTGAIVGIQDMGAAGLTCSTCEMGARGGVGLQVELDHVPQRETGMTAYEI
                     MLSESQERMLLVADKGREQEVLRVFEKWGLDAVIVGKVIAEPRLRILHHGELVADIPN
                     QSLTDDAPVYHRPVGEWKAPFPAGPTRSALDKLGFEDNFSVRDNTEDLRTLLASPNIC
                     SKRWVHEQYDSMVQTNTVQGPGGEAGVMRIKGTKRGLAMALDGNGRWCYLNPKLGAKH
                     AVAEAARKVACTGATPIAATNCLNFGNPEKPEIMAQLSAAIDGIAEACLALGTPITGG
                     NVSLYNETRGEGIYPTPVLGIVGLIEDITKTVPAGFRQEGDAVLIIRAAKQPPIDERL
                     REFGSSEYAKVVLGEIWGTPPSLDLAAEAALHKCLAELASAGLLHSASDISDGGIAVA
                     LAEAGFRNGIGVLTSLQETADLPAAVALFSEPATQVIVSCAMASVEKIKAIVQKYSEL
                     QVENLGKTVKDSFRIQVNGKTLVDERISGLEHLWSHALEAALHEEVLA"
     gene            1854..3338
                     /gene="purF"
                     /locus_tag="VFA02_01625"
     CDS             1854..3338
                     /gene="purF"
                     /locus_tag="VFA02_01625"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZU08574.1"
                     /translation="MVEGIPGEETRVTGSCEAGVGTPQDDKLREECGVVAIHGHPDAA
                     RQAYLGLYALQHRGQESAGIATADGTNLFNIKGMGLVADIFTEEVLSKLTGNMAIGHT
                     RYSTTGDSALLNAQPIRVDSTKGLIAIAHNGNLVNLGNVRARLEREGAFFQTTSDSEI
                     IVQLIAHSKENTLVEAIADSLRQVDGAFSIVMMTRDRVFAARDPRGFRPLSMGRIENP
                     DGPDTIVFASETCAFDLLRAKFERDVKPGELVMVTTDGVTSVQYASGIPQSSCIFEHV
                     YFARPDSKIYNRWVQESREEMGRQLARESHVDADLVVPVPDSGVTAAIGYAAESGIPF
                     RFGLIRNHYVGRTFIEPEQRVRDFGVKLKLNPVRNLLEGKRIILIDDSIIRGTTSRKI
                     VRMVRAAGAKEVHMRISCPPTTSPCFYGVDTPRKKELIAANNSIEEIRQFIEADSLAY
                     LSLPGLQKACDGGEGNRFCVACYTGDYPTDWVDVEEILPATVSQ"
     gene            3469..3735
                     /locus_tag="VFA02_01630"
     CDS             3469..3735
                     /locus_tag="VFA02_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZU08575.1"
                     /translation="MPAKKKSSGRKYGSKASKSVEREMRAMKQGKLKSGRSGKKVTNP
                     KQAVAIGLSEARKKGAKVPSPPKKKSTKKKSSAKKSSKKKSSRK"
     gene            3914..6424
                     /gene="galA"
                     /locus_tag="VFA02_01635"
     CDS             3914..6424
                     /gene="galA"
                     /locus_tag="VFA02_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HZU08576.1"
                     /translation="MPRISRRDLFASGLALSASSLISRSAWARTAALFDDPASAVPAL
                     APREQLLFDFGWKFTFGNSNDPSKDLGFGFSQDDFAKTGDFKFAKAGFDDSKWRELNL
                     PHDWAVELPFVRDDELKSHGYKPLGRRYPETSVGWYRREFDVPASDLGRRIWVEFDGA
                     FRDVLVFVNGCFVGRNNNGYIPFRFDLTDFLAYGQKNYIVLRVDASFGDGWFYEGAGV
                     YRHVWLTKMDAVHLGKWESTVRPKLNGNSATLELSTIVQNEGRQPANAKVSWKIQDAK
                     GATVATAEAPAQSIAVDGSASYTATAELSNPALWSVDEPNLYTAIVTVETDGSVRDAE
                     RVPFGVRTAVFDADKGFFLNGKPLKIQGTCNHQDHAGVGAALPDRLQWYRLGVLREMG
                     CNAVRTSHNMPTPEWVEACDRMGVMMMCETRQMSSSEEGLRQLELMVKRYRNSPSIII
                     WSIGNEEWLLQDKMAEQGAKIGASMVRLCHELDPTRVVSAAVNGNNEKGLSEALDIIG
                     FNYNTKKYPEAYHKEHPKRPIFGSETSSAISTRGEYASDPLRNTVNSYDGVVPWGETP
                     EQWWTFYGTHEWEAGGFAWTGFDYRGEPTPYGWPSINSQFGIVDMCGFPKDYFFYYKA
                     WWRKEPSLHLFPHWNWHGREGDEIPVWVYSNLDEVELLINGKSAGRQKVPQLRHVEWK
                     VRYEPGVIEARAYKGGKVVLTEKRETTGPAASIRLTADRTEINADGEDLALIKVEALD
                     KEGRLVPVADSKIAFNITGAGKLIGVGNGDPNCQESDKEPKRSLFHGLAQVIIQSTKQ
                     PGEIQIEASKDRESDGPELTPARLVITAKQTVPRPSVP"
     gene            6500..>7108
                     /locus_tag="VFA02_01640"
     CDS             6500..>7108
                     /locus_tag="VFA02_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08577.1"
                     /translation="MTTRRAFLFRSVPSAAALTITAPKLAFASPSHNSAPAAIADIDT
                     SSSELRPYIERFSADQELLRRYYSIPFAPQTRDRMHKFYGEWQSRLDEVNFDSLSEDA
                     RIDYILFRNYLDRELAHMEEDAQFQAETAPYTPFAGTIISLFFAQREIEPIDGKQSAA
                     TLAQIEQACKDKQKELEASLSANKEDTKSLAYQRRREAANRAV"
BASE COUNT         1636 a         2076 c         1984 g         1412 t
ORIGIN      
        1 gtcgtccacg gcattgccgg atatggaaac tgcttcggcg tgccgaatct gggcggcgag
       61 acccgctttg aggactgtta ctcgggcaat ccgctggtga acgcctttgc gctcggcctt
      121 gtccggcgcg atgagatttt ctatgccagg gccactggcg ttggcaatcc agtgatttac
      181 gtcggggcga agaccggacg cgacggtatc cacggcgcga cgatggcaag cgaggagttc
      241 aaggaaggtt ccgagcagaa gcggcccaat gttcaagtgg gcgacccctt tatggagaag
      301 ctgctgctcg aggcctgcct tgaggcaatg aagaccgggg ccatcgtcgg cattcaggac
      361 atgggcgcag caggcctcac ctgctccacc tgcgaaatgg gcgctcgcgg cggcgtaggt
      421 cttcaggtcg aactcgacca cgtgccgcag cgcgaaaccg gcatgaccgc ctacgagatc
      481 atgctgagcg aatcgcagga gcgcatgttg cttgtcgcag acaagggccg cgagcaggaa
      541 gtgcttcgtg tcttcgagaa gtggggcctt gacgcggtga tcgtcggcaa agtcatcgcc
      601 gaaccccgcc tccgcatcct gcatcacggt gaactcgtcg cagacattcc aaatcaatcg
      661 ctgaccgacg atgctccggt ctatcaccgt ccggtgggtg agtggaaagc gccttttccg
      721 gcagggccga caagaagcgc gctcgacaag ctgggctttg aagacaactt ttccgtccgc
      781 gacaacaccg aagacctgcg cacgctgctg gcttcaccca acatctgctc caaacgctgg
      841 gtgcatgagc aatacgactc gatggtacag accaacacag tacaaggccc gggcggagaa
      901 gccggtgtga tgcgcatcaa aggcacaaag cgtggtctgg ccatggcgct cgatggcaac
      961 ggacgctggt gttatctcaa tccgaaactt ggcgcaaaac acgccgtcgc agaagccgcg
     1021 cgcaaggtgg cctgcacagg ggcgacgccg attgcggcga ccaactgcct gaacttcggc
     1081 aatccggaaa aacctgaaat catggcgcaa ctctccgctg ccatagatgg cattgcagaa
     1141 gcctgccttg cattgggtac gcccatcacg ggaggcaatg tctcgctcta taacgaaacg
     1201 cgcggtgagg gcatctatcc cacacccgtg ctgggcattg tcggtctcat cgaagacatc
     1261 acgaagaccg tgcccgcggg cttccggcag gaaggcgatg cagtgttgat cattcgagcg
     1321 gcgaaacagc ctccgataga cgagcgcctg cgtgagtttg gctcatcaga atacgccaaa
     1381 gttgtgctcg gcgaaatctg gggaacgcct ccatcgctgg atctggctgc tgaagccgcg
     1441 ttgcacaaat gtctcgcaga gctggcaagt gccggtctgc tgcactcggc aagtgatatt
     1501 tcagacggtg gcattgccgt tgcgctcgca gaggccggat ttagaaacgg tattggtgtt
     1561 ctgacttcgc ttcaggagac cgcagatctg cccgcggcgg ttgcgttgtt cagtgagccg
     1621 gctacacagg tgattgtctc ctgcgcaatg gcttctgttg aaaagatcaa ggcgatcgtc
     1681 caaaaatatt ctgaattaca ggttgaaaat ctcggcaaaa ccgtgaaaga tagcttccgc
     1741 attcaggtga atggcaaaac attggtagac gaacgtatct ccggcctcga acacttgtgg
     1801 tcccatgcac tggaggccgc cctgcacgaa gaggtgctcg cgtgaaatac ctcatggttg
     1861 aaggtattcc cggagaagaa accagggtaa ccggttcttg cgaagccggt gtggggaccc
     1921 cacaggatga caagctccgc gaagagtgcg gcgtggtcgc catccacgga catcccgacg
     1981 cagcccgtca ggcatacctc ggcctctatg cgcttcagca tcgtggacag gagtccgccg
     2041 gaatcgctac tgccgatggc actaatctct tcaacatcaa aggcatgggt ctggtcgcgg
     2101 acatcttcac cgaagaagtc ctttccaaac tcactgggaa catggccatt ggccacacgc
     2161 gctactctac aaccggcgac tcggcgctgc tgaatgcgca gcccatccgc gtagattcaa
     2221 ccaagggcct tatcgccatt gcccacaacg gcaaccttgt aaacctgggt aatgtgcgcg
     2281 ccaggctcga acgcgaaggc gccttcttcc agaccacaag cgattctgaa atcatcgtcc
     2341 agctcatcgc gcattccaaa gagaacacgc tggttgaggc catcgccgat tcgcttcgcc
     2401 aggtagacgg cgctttttcc atcgtgatga tgacgcgcga ccgcgtcttt gccgcccgtg
     2461 atccacgcgg cttccgtccg ctctcgatgg gccgcattga aaaccccgac ggcccggaca
     2521 cgattgtctt tgcctcagaa acctgcgcct tcgatctgct gcgcgcaaaa ttcgagcgcg
     2581 atgtaaaacc gggcgaactg gtcatggtca ctacagatgg cgtgacctcc gtccagtatg
     2641 cgagcggcat cccccaatcg agctgcatct tcgagcatgt ttactttgcg cggcctgaca
     2701 gcaaaatcta caaccgctgg gtacaggaga gccgcgaaga gatgggccgc cagcttgcgc
     2761 gcgaatcgca cgtggatgcc gacctcgtcg tcccagtccc ggacagtggc gtgaccgcag
     2821 cgataggcta tgcagccgag agcggcatcc ccttccgctt cgggctgatc cgcaaccact
     2881 acgtggggcg caccttcatc gaaccggagc aacgcgtgcg cgatttcggc gtgaagctca
     2941 agttgaaccc ggtgcgcaat ctgctcgaag gcaagcgcat cattttgatt gatgattcca
     3001 tcattcgcgg caccaccagc cgcaagatcg tgcgcatggt gcgtgcggct ggcgcaaagg
     3061 aagtccacat gcgcatcagt tgcccgccga ccacatcgcc gtgtttctat ggcgtggata
     3121 ctccgcgcaa aaaagagctg atcgcggcca ataattcaat tgaagagatc cgccagttta
     3181 ttgaagcaga ctcgcttgcc tatttgtcac ttccagggtt acaaaaagcc tgcgatggtg
     3241 gtgagggcaa ccgcttctgc gtcgcctgtt acacagggga ctatccaacc gattgggtgg
     3301 atgtagaaga gattctgcct gcaacagtat cacaataaaa aactgtcatc atggtcttca
     3361 gcaccgcgaa ggaaagacca tgcttttctc ctcgcgcata aactgcatgc ccacatcgca
     3421 caaccctcct gctctcttct gcttcccatt tctctggagg tgcacacgat gcccgcaaaa
     3481 aagaaatcca gcggacggaa gtatggttca aaggccagca agtcagttga gcgcgagatg
     3541 cgcgccatga agcaaggcaa gctgaaaagc ggtcgcagcg gaaagaaagt cacaaatcca
     3601 aagcaggcag ttgcaattgg cctttctgaa gcgcgcaaaa aaggcgcaaa ggtgcccagc
     3661 cctcctaaaa agaagagcac aaaaaagaag agttctgcaa agaaaagctc aaagaagaag
     3721 tccagccgga aataacctct gcccttttgc gcgcggatgg gcgatgcctc tgctgttcgc
     3781 ccgcatttcc gcgcggttcc cctctctcaa caggcagaat ttccttcccg aacagatttc
     3841 ataccaacag gactgccctt tacagtattc taggcagcag atttgttctt caaaatttct
     3901 ctgcgaggtc tttatgccgc gtatctctcg ccgtgacctg tttgcctccg ggcttgctct
     3961 ctcggcctct tctttgatct ctcgctctgc gtgggcaaga acagccgccc tctttgacga
     4021 tcccgcatct gcggtccctg cacttgcacc gcgtgagcag cttctttttg atttcggctg
     4081 gaaattcacc ttcggcaaca gcaacgaccc atcaaaagac ctcgggtttg gcttcagtca
     4141 ggatgatttc gccaagaccg gtgactttaa gtttgccaag gcgggcttcg acgattcaaa
     4201 gtggcgcgag ttgaatctgc cgcatgactg ggccgtcgag ctgcccttcg ttcgggacga
     4261 tgagctgaaa tcgcatggct ataagccgct tgggcgccgc tatcctgaaa ccagcgtggg
     4321 atggtaccgg cgcgagtttg acgtccctgc cagcgatctc gggcgccgca tctgggttga
     4381 gttcgatggg gcttttcgtg atgtgctcgt ttttgtaaac ggttgctttg tgggccgcaa
     4441 caacaacggc tacattccct tccgcttcga ccttactgac tttcttgcct atggccagaa
     4501 gaactacatt gtcctgcgtg tagacgctag cttcggcgac ggctggtttt acgaaggcgc
     4561 aggcgtctat cgccacgtct ggctcaccaa aatggacgcg gtgcatctcg gcaagtggga
     4621 gagcacggta cgcccgaagc tcaacggcaa ttcggccacg cttgaactct cgaccatcgt
     4681 gcagaacgaa ggccggcagc ccgcaaatgc caaagtcagc tggaaaatcc aggacgcgaa
     4741 gggcgcgacc gtagcgacag ccgaagcgcc cgcgcaatcc atcgccgtag acggctcggc
     4801 gagttacacc gccacggctg aactctcaaa cccggcgctc tggtcagtag atgaaccgaa
     4861 cctctacacc gccattgtta cggtcgaaac tgacggcagc gtgcgcgacg ccgagcgcgt
     4921 gccttttggc gttcgcacag ctgtgtttga tgcagacaag ggcttcttcc tgaacggcaa
     4981 accgctcaaa attcaaggca cctgcaatca tcaggaccac gcaggtgtag gcgcggcgct
     5041 gcccgaccgc ctgcaatggt accgtcttgg ggtcctgcgt gaaatgggct gcaatgctgt
     5101 ccgcacttcg cacaacatgc cgacaccgga gtgggtcgaa gcctgcgacc gcatgggcgt
     5161 gatgatgatg tgcgaaaccc gccagatgag ttccagcgaa gaagggctgc gtcaactgga
     5221 gctgatggtg aagcgctatc gcaactcgcc ttccatcatt atctggtcga ttggaaatga
     5281 agagtggttg ttgcaggaca agatggccga acagggcgcg aaaatcggcg ccagcatggt
     5341 caggctctgc catgaactgg acccgacccg tgtcgtctct gctgccgtga atggtaacaa
     5401 cgagaagggt ctctcggaag cgctggacat catcggcttt aactacaaca cgaaaaaata
     5461 tcctgaggcc taccacaagg aacacccgaa acggccgatc ttcggctcgg aaacttccag
     5521 cgccatcagt acgcgcggcg agtatgcatc tgatccgctg cgcaataccg tcaacagcta
     5581 tgatggcgtc gttccctggg gcgaaacacc cgaacagtgg tggaccttct acggcacgca
     5641 tgagtgggag gctggcggct tcgcctggac tggcttcgat tatcgcggcg agcctacccc
     5701 ctatggctgg ccttccatca actcgcagtt cggcattgta gacatgtgcg gcttcccgaa
     5761 agactacttc ttctactaca aggcctggtg gagaaaagag ccatcgctgc acctgttccc
     5821 gcactggaac tggcacggaa gagaaggcga tgaaattccc gtctgggtct attccaacct
     5881 cgatgaagtc gagctgctca tcaacggcaa gagcgcaggc cgccagaagg tcccgcaatt
     5941 gagacacgtg gagtggaagg tgcgctatga acctggcgtc atcgaagcgc gtgcttataa
     6001 aggcggcaaa gttgttctga cagaaaagcg cgagaccacc ggccctgccg catccatccg
     6061 gctgaccgca gaccgcactg aaatcaacgc tgatggcgaa gacctggcgc tcatcaaagt
     6121 ggaggccctc gataaagaag gccgtcttgt cccggttgcg gacagcaaaa ttgcattcaa
     6181 catcactggc gcgggaaaat tgatcggcgt tggcaacggt gaccccaatt gccaggagag
     6241 cgacaaagaa ccaaagcgct ctctgttcca tggactggcc caggtgatca tccagagcac
     6301 aaagcagccg ggcgagatcc agattgaggc gtcaaaagac cgtgaatcgg atggccctga
     6361 actgacgcct gcaaggctgg tcatcacagc aaagcagact gtaccgcgtc cttcggttcc
     6421 ataaacaaaa gagcggcgag tgtcgtcttc accggacacg gaatctcaaa ttgtatacaa
     6481 ttgtttttcc ggagaagcca tgaccactcg ccgcgccttc ctttttcgca gtgtgccttc
     6541 tgccgctgct ctcaccatca ccgcgccgaa acttgcgttc gcatcgccat cccacaactc
     6601 tgcgccagcc gcaattgcag acatagacac ctcttccagc gaattgcggc catatatcga
     6661 acggttctcc gctgaccagg agcttttgcg gcgttattac agcatcccgt tcgcgccgca
     6721 aacccgcgac cgcatgcaca agttttatgg tgagtggcaa agcaggctcg acgaagtaaa
     6781 tttcgacagt ctcagcgaag acgcgcgcat agactacatc ctgtttcgca attatcttga
     6841 ccgcgagctg gcacacatgg aagaagatgc gcagttccag gccgagaccg caccatacac
     6901 tcctttcgcc ggcacaatca tctccctttt cttcgctcag cgcgagatag aaccgataga
     6961 cggcaagcaa tcagcggcca ccctggccca aattgagcag gcctgcaaag acaagcagaa
     7021 agaactggaa gcgagtctaa gcgcaaacaa agaagataca aaaagccttg cttaccagcg
     7081 ccgccgcgag gccgcaaacc gcgccgtg
//
LOCUS       DASRBV010000038        29617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_205522, whole genome shotgun sequence.
ACCESSION   DASRBV010000038 DASRBV010000000
VERSION     DASRBV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 29617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29617
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205522"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VFA02_01645"
     CDS             complement(<1..1243)
                     /locus_tag="VFA02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HZU08578.1"
                     /translation="MPRFCWSLVFISLFTSGFSTYAQDARHVTEPVVPPPCVVLKAQL
                     QANAGKLSERDEQQQDTKRIQQAIASCQPGKAVELRADGAKNIFLSGPLQLKKGVTLL
                     VDANTAIFASRNPRDYDITPGSCGIVSEKGHGCRPLIAVNNAPDSGVMGEGSIDGRGG
                     EKLIGQDETWWDLAHRAKIEDKGQSCFRLIEVNHSDNFTLYKITLRNSPNFHVAVNQT
                     NGFTAWGVKIMTPKTARNTDGIDPGSSTNVTIAYSYIHTGDDNVAIKSGKAGAATNIS
                     VLHNHFYTGHGMSIGSGTEGGVRNILVQDLTIDGADNGIRIKSDRSRGGLVQDVTYED
                     VCIRDTRNPIIFTPLYTTFSGNALPVYRDILLKNVHVLTPGTYTFLGLDAQHKLGVTL
                     DNVFADGLEQSKIIAKDAEITL"
     gene            1572..2348
                     /locus_tag="VFA02_01650"
     CDS             1572..2348
                     /locus_tag="VFA02_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HZU08579.1"
                     /translation="MKPSLPVPQNPALPTLFLIGDSTVRNGNGTGANGLWGWGDILPE
                     YFDLAKINVVNRARGGRSSRTFITEGLWDETLALIKPGDYVVMQFGHNDASPLDDAAR
                     ARGTLPGIGDETRDIWNPIMKKQETVHTYGWYMRKYVKDTLAKGATPILCSPIPRNNW
                     KDGKVVRNEDSYGGWARQIANEFHIGFVNLDEIIAETYEKMGEAKVAPLFPADHTHTS
                     REGAEINAEAVVSGMKALPGNPFKKYFSPKAASVPKWKGN"
     gene            complement(2403..3710)
                     /locus_tag="VFA02_01655"
     CDS             complement(2403..3710)
                     /locus_tag="VFA02_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HZU08580.1"
                     /translation="MMLPQKLFCVSIVFAFLICCCSGASAQSWQFTCTSTKHEKEKGA
                     AELTVHAVYSPEKQFGFDPGTVPDVKDSACFSDKPFFFSLTVPDGNYQVTVEMGGGPN
                     ASVTTVKAESRRLMLLSVATSPHQTTTRRFNVNVRTPQIEGDGEVHLKPREVGSLDWD
                     GKLTLEFIGRNPSFRSITIQKADVPTLYLAGDSTVVDQDKEPWAAWGQMLPVFFNDTV
                     SISDQAESGETIHSFTVENRFAKIFSTMRAGDYLFMQFAHNDQKPGKGFVSISDYKQY
                     LIKYIQMARSKGATPVLVTSMNRRTFDANGKIIQTLGDYPEAMREVAKEQNVALIDLN
                     AMSKTLYEALGPEGTLKAFVHFPANTFPDQPLPLKDDTHFTDYGAYELARCVVKGIRE
                     AGLPLASAIVKGEAEFDPAYPDNPNALKLPLDPQVGTVTPYGR"
     gene            complement(3823..5076)
                     /locus_tag="VFA02_01660"
     CDS             complement(3823..5076)
                     /locus_tag="VFA02_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZU08581.1"
                     /translation="MESSFLKHAIFAYPTLFPRLCVRKWKITIVPILLAFFSFVPCFA
                     QAQYPQPTPDQRAGIDKDNARHFGDDPVDGGPMAKDLSPALKVKDIHKAMTKVADWEL
                     KTWQPWFDRIWTSSVLYTGLMAASASTGDSRYRDAVREVGKKYNWELRSHLPNADDQS
                     VGQSYLELYFIDRDPAEIAPTRAELDALYPLEKDKQGRIQWWWCDALFMAPPVWARMY
                     AATNDGKYLDYIEKHWDETQSLLYDKQEHLFARDATYLDKREKNGQKIFWSRGNGWVM
                     GGIVRVLEYMPKNDARREKYVELLREMADRVKSLQDPKDGLWHSSLLDPADYPLPEIS
                     GSALFTYAMAWGVNEGILDRKVYKPVVARAWKGMLEHIYADGRLGCIQQTGAAPAWYR
                     PTASYDYGVGAFLLAGSEVARMTKR"
     gene            complement(5066..6307)
                     /locus_tag="VFA02_01665"
     CDS             complement(5066..6307)
                     /locus_tag="VFA02_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HZU08582.1"
                     /translation="MKTSMFLAKCSAVLALVSSSFAVLAQQTPKEWVDPDTGHRIVQL
                     SEEPGSGSLYFNLNGYTPNGDKLLITTPHGMAFVDLATRRIEPLNHDPHAHPIMVGHK
                     TGRVYFARDGAVWYLDPETKKETKVADMPPHGSIATVNADETLLAGTYVESQGIEKAQ
                     HLSPPPQTRDGRGHMLEERYNAHLPMALFTLNIKTGEVKVLNRSTEWLNHLLFSPTDP
                     NLLMFCHEGPWQKVDRIWLIHTDGSGLMLVHKRRMQMEIAGHEFWSADGKTIWYDLQT
                     PRGEDFWVGGYNVYDGHRTWWHLQRNEWSVHFNVSPDGTLFAGDGGDSKMVAHAPDGK
                     WIYLFHPKPVPNTGVQEKDLIEAGAFEAEKLVNMSKHDYALEPNVNFTPDGKWIVFRS
                     NMSGAEQVYAVEVAKEPGHGK"
     gene            complement(6498..10325)
                     /locus_tag="VFA02_01670"
     CDS             complement(6498..10325)
                     /locus_tag="VFA02_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08583.1"
                     /translation="MAVACFSVSGWAQTAGEGAIRGTVTDPSGAVVPNATVTAVNTAS
                     GTQYTRTTSSDGLYVISPVLPGTYTVTVTAAGFQSFKQENLTVNALTVTPLDVKLSLG
                     SQTQQVTVTAAPPALETTTATLGGVMENREYQNLPILMGGQQRDPTAFATLMPGTQGG
                     TRAPVIGGTGNYLAEVYLDGIPTTTINQQGDNRVIFNAVPVEAVDQFQVVTSVPGAEY
                     QGAGLMNFTVKSGGNQYHGTVADFVRARMFDTWGFSAKALTVKNAAGQTVPAPKPDEH
                     QNELVGAVGGPIPFTKNKGFFFVAYDRYHGRSGVNPNTLTVPTMKMRQGDFSELLTTT
                     NGITTGQIYDPTSEATCTAHSTNGPCRYQFGYGYGGIPGPNGNPVPTGSPNVIPQSYL
                     SPIAQYMQKFLPEPTNSQITNNYLGGVPSGYDNWEITSRVDYNLTGSQKLSGVFAYGY
                     RENVPFTVGNSGVVLPPPYTNGGKAIIKPVFADIEHSIVITPYLVNQFKFGYTRFAQP
                     VTAITDGVKQWEAVTAGITNLPQGQASTEFPGASFSASTLFPLVQSAWTANGASGATQ
                     NTVPNAFTLVDNLQWVKGNHSMTFGVQMQWLQDNLTPLAGPSGILTMGYNANSTANFA
                     PGSTSLATNTSGYSYASYLLGAVGGSPSIAIQPFSETGGRYHPISPYFQDDWKISKNL
                     TVNLGLRWDYFPPFKEVLDRWSFLNPTGINPATGTPGVLEFAGHRGADISCNCRTPVH
                     TYWKNFGPRIGFAYQPDPKSVVRGGFAIAYSHAGGVGGRAGAGNGTGQLGFTVNATAP
                     IELTTGSGAGPSFYLNNSPYFQSIGLANTAFGGPGFALPTPSAPGTASLTLSTGNYVD
                     PSTGKFVTASSAPGYADPYLSGRAPEFIFYSFGIQRAITDTLVASANYVGTQSHFVVP
                     GSSNIRGYWSNQLDPAYLAALGGVADAKGNPILNDAATPANVAIAKQAMPGLNIPSVF
                     VNNNTKATIATVLRAFPQYSGVSDTWGQNSANISYNSFQFSLQQRPWHGLSYMVNYTY
                     SKNLGDDGTFRSGFAIPGAAMSNGRSYKMDAIERSYTTLSMPQTLSAFGVYQLPFGKG
                     GIGNDSALVRALAGGWQLSSIYTYHSGTPLAITWGGCTAPGQGQCMPDVNPAFFGRGS
                     ARKNGSWGKGITAAQLGSTIQYIDVNAFSTPATFGNATKTPIYKIGDAPRTAPWGLWG
                     PSDWNMDATMQRAFQLTERWQLVVRADCSDVFNHNTKGGISTAWGPGSTTFGTVGSAR
                     GNRDWQFSGRINF"
     gene            10714..12027
                     /locus_tag="VFA02_01675"
     CDS             10714..12027
                     /locus_tag="VFA02_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HZU08584.1"
                     /translation="MILLTLVLSASAERRISVNDYGAKGDGIALDTHAIQSAIDAASK
                     AGGGTVTFRPGTYISGSIFLRSHVHLEVGNGVIIRGIQDTNQYPMMPTRVAGIEMTWP
                     AALVNVYKQEDVSISGPGTVDGNGKYWWDAYWALRHEYEPKGLRWAADYDAKRPRLIQ
                     IFDSKKVSLKNIRLERSGFWTVHICYSNDVTLAGVTIRNNIGGKGPSTDGIDIDSSRH
                     VLVEHADIEVNDDALCLKAGRDWDGLRVHRPTEDIVIRDSTVRAGAAGFTIGSETSGG
                     FRNVDVYRLTVLAPVPSGILFKSDRTRGGGAEDIRIHDITMDGVAIPIHFTMNWNPKY
                     SYATIPAGITNPPDYWKTITHPVEPPERGLPHFRDVKIWNIRATGAKKAFVVSAYPEA
                     PLTDFDFSHIDISAQNAGSITDAKNWKWKDVKIQTEDGSKLKLPE"
     gene            complement(12064..12999)
                     /locus_tag="VFA02_01680"
     CDS             complement(12064..12999)
                     /locus_tag="VFA02_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZU08585.1"
                     /translation="MSELDPEQLASAPLAYDNPAFLNSPDGRIVRILSEYLEPLARFR
                     RERIQDTVVFFGSARFRALDEANHQLELLENTGSQRPAPQEEQPATDGAVTELQLRRA
                     EAAVEMAHYYEDARRLAYLLTDWAKGLKSRRHRFVVTSGGGPGIMEAANRGAYEAGGK
                     TIGLNIKLPFEQQPNRYITPALNFEFHYFFMRKYWFAYLAKALVVFPGGFGTLDEMFE
                     LLTLAQTQKLAKKMTIVVYGSAYWKKVLNFDVLVEKGAISPRDVELFQFADTPEEAFQ
                     ILKDGLTKNHLLPEEQAAEADDEMALRPEIAKTRR"
     gene            complement(13003..13332)
                     /locus_tag="VFA02_01685"
     CDS             complement(13003..13332)
                     /locus_tag="VFA02_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08586.1"
                     /translation="MSYQPNLFSSNTPHGPAGPESKPAIPEEHRTPVWLRRIDLFLRV
                     VVRLYLGLLIVALPWTHLWTENHLFNLVSQVAWLASNGAVRGVVSGLGLLNIWIAITD
                     AIHYREP"
     gene            13510..14235
                     /locus_tag="VFA02_01690"
     CDS             13510..14235
                     /locus_tag="VFA02_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08587.1"
                     /translation="MPPAFAGNIQVPTAPVDHSSDHKLPNLRSLNPSAIHTTAALGGT
                     IGPHVTLVHGDEWTIITGSEKRTIQNDQTIEIKGNEAHTTTKNLTYKIIGQTVDTRIG
                     AHYQTNNSPRFETYVHTLTQDHHEKMIVHQPTTLMDIINLYFGRKWVSLNATGASFSA
                     TGTSASLTSLSGSGTVMSASNTVFSSSRESFSIKDLDIGLHVRGMITELKAMKLKAAL
                     AHLKAIIGNFNAGIAANMSSPFS"
     gene            14306..14749
                     /locus_tag="VFA02_01695"
     CDS             14306..14749
                     /locus_tag="VFA02_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08588.1"
                     /translation="MGDKLTIAVPADLPLFIIFLLPLLILFGVYREMNTSRGRKKCVV
                     YMLINIVILSMIFTPAKVELDARQGTATVRTVMFFVPRHHVIPLSSVQGAMVKTSELT
                     EALALVLTDGHAMQLTPFDQVRGTDVAAAAINDFLRTHGGVGKPY"
     gene            14797..15693
                     /locus_tag="VFA02_01700"
     CDS             14797..15693
                     /locus_tag="VFA02_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08589.1"
                     /translation="MSLRKKRSRIDPIRPVTDLSPFLRAAQITDFSLYWAPVTEYPVP
                     LNQREWMREFLKRLAGHLKQKDELREEWFLQFKALYPHLIDRFVECASHYVPVTGLGF
                     VPGAVPAELPDFEAVKKEVEKASKDGRHVEVQQWMPDHVHWFAAKQPEEQRRDFFGHG
                     GMFALYMRPNTKAARVFPQLPRFMTTHEAYTPDLERQFAMLNSLQDDFLAKSKEIFGT
                     PFRQKPGYRGLAFIVPLLSSRSFLDSTPEQRGTWFRLFQGYFIESRQDNGVLFASSDF
                     EFDEDLISILEGMRSDGFFYRT"
     gene            15851..16417
                     /locus_tag="VFA02_01705"
     CDS             15851..16417
                     /locus_tag="VFA02_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08590.1"
                     /translation="MQLSVWLLMDSLHKDGAVHALSRHGAEWLPDFGFGLWPDREPPR
                     MYEERDFLDLEFGAEMPRLMHSVAHITTENQDTVTHAWETMFGTGSTVLALSSVSADN
                     ILATMRVAALEKIQDETFRSFPWYFSLIGQKAMEAGPVLLDSWLGPVELYVRESEEDG
                     SIVLLGRSDFSLALEAAGFHLIEEPPQK"
     gene            complement(16425..18239)
                     /gene="typA"
                     /locus_tag="VFA02_01710"
     CDS             complement(16425..18239)
                     /gene="typA"
                     /locus_tag="VFA02_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZU08591.1"
                     /translation="MSTSIRNIAIIAHVDHGKTTLVDAMLRQSGTFRANEAVAERVMD
                     SNDLERERGITILAKNTAIYYKDTKINIVDTPGHADFGGEVERALKMVDGVVLLVDAS
                     EGPLPQTRYVLSKALEAKLTPVLVINKIDRPDARPQEVLNEVYDLFIDLDATEDQLDF
                     PVLYTNGKLGLASTDAAVPGKDLQPLFETILKTMPPPNGDANGPLQILVTNLDYSDYL
                     GRIAIARVFNGTLKTGEEVYISKRDGALQKTRITKLFSFAGLKRVDIETTEIGDIVAI
                     AGVEGITIGETITGIENPAPLPPIVIDEPTIAMQFMVNNSPFAGREGQFVTSRNLRER
                     LEKELLTNVSIRVEDTDSPDAFKVLGRGELQLAILIEMMRREGYELMVGRPEIVTRTI
                     DGKVMEPVEHLTIDIPENFVGTVIERLGPRKGEMVKMHNHGSGRVRLEFRVPSRGLIG
                     LRSELLTETRGTIVMNSLFDGYMPYQGEIPQRSTGALISDRAGTITAYALDGLQDRGV
                     LFVQPGVETYEGMIIGEHSRDNDLDVNAVREKKLTNMRASSADEAIRLAPPRLLNLEQ
                     AIEFIADDELVEVTPKSLRLRKKVLQANRRPKRNAVPA"
     gene            complement(18420..19094)
                     /locus_tag="VFA02_01715"
     CDS             complement(18420..19094)
                     /locus_tag="VFA02_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08592.1"
                     /translation="MFKRTLFSFLALAAMVSAASAQTQGTVSSPNQQPQVAPNYALAG
                     VHYDYKWELYGGFAYSHFNAGPNLLQGANLGGFDVQAARFFTRRWAAAANVRGYYGTS
                     GVVPNVYNIKGPFVSEHMFLAGPEWRGPSNEHASLTLHALVGGAYGNFQHDIGNVPPA
                     ELGLFSNQLTFGGAFGGSLDLNRSPRLVFRISPDATITKFGSGGIQDQFAISVGIVYR
                     MKRGLK"
     gene            19251..19889
                     /gene="infC"
                     /locus_tag="VFA02_01720"
     CDS             19251..19889
                     /gene="infC"
                     /locus_tag="VFA02_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZU08593.1"
                     /translation="MFAFNFRRKPIAQLDKRSAKQFIRINERIRAREIRVIDENGEQL
                     GILQPFEALKIARERGLDLVEVSPNAVPPVCRIQDYGKYLYEKDKSERAARKKQKVIT
                     VKEVKFSVTVDEHDYQTKKNQAIRFLHEGDKVKASLRFKGRQMAHRELGYNIINRLIQ
                     DVGDAGTVEFMPRMEGTTLHAILAPAKKEAPKKAAPPKPPAPQTAPAAQTQA"
     gene            20151..20588
                     /locus_tag="VFA02_01725"
     CDS             20151..20588
                     /locus_tag="VFA02_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZU08594.1"
                     /translation="MPDTTGTLKTHTEYLFFHTRKRREIVHITPQLEKIVVRSGIRDG
                     FCFVSPMHITAAIYVNDHESGLIADIEEWLETLAPARPDYRHHQTGEDNADAHLKSLL
                     LHHEVTLPITDGRLDLGTWQRAFYAEFDGQRRKRVIVKLMGVA"
     gene            complement(20560..21411)
                     /locus_tag="VFA02_01730"
     CDS             complement(20560..21411)
                     /locus_tag="VFA02_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZU08595.1"
                     /translation="MKQKGQKGHAPNHERWLVSYADFITLMFAFFVVMYASSKADMKK
                     QAAVAQAINDAFKSLGLFQSVSKDQTPKAAALSASPDAQVSPVNVVLGEQMLASPEVR
                     QDLERIKKQLEKLLSNQIAHHVVALRIGRDGLVISLREAGFYDSGSATPHPDSIRTLD
                     AIADALRPTPYDIRIEGHTDNVPIHNDQFDSNWELSTARATRLAKMFIVAHHFSPVRL
                     SASGYAEFHPVANNATPDGRSQNRRVDIIVLPSMTIPRAPAMNVSQARPEAVNAALTK
                     PLPSASQ"
     gene            complement(21408..22256)
                     /locus_tag="VFA02_01735"
     CDS             complement(21408..22256)
                     /locus_tag="VFA02_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HZU08596.1"
                     /translation="MMQRNGAAGTGKSKFQGKSMDKSSFTGVVLALAGILAGLLIEGG
                     HIGQVLQPTAAMIVLGGTMGAVMLQFPLATVGSAFKRLAQVFFEKKNDADATVKLLLG
                     FANKARRSGIVSLDQDLASIADPFMKQALMLAVDGTEPAELRKIMELHLENEAQMEDT
                     IPAVFESAGGFAPTIGIIGAVMGLIQVMGHLDHMDEVGRGIAVAFVATIYGVGSANIF
                     FLPAAGKLRYRVADERRLKEMVLEGVISILEGMNPRMIEIKLQGFHPAAGKKGKSAKP
                     VEAEAA"
     gene            complement(22222..22554)
                     /locus_tag="VFA02_01740"
     CDS             complement(22222..22554)
                     /locus_tag="VFA02_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZU08597.1"
                     /translation="MIELTRLNGSPMVVNSDLIKYVESSPDTTLTLLHGEKIVVAEPC
                     AEVVRRITAYRTKLLADAMKQATTDDLGAVAAASAKSTGLRIVAANSAMDEVPGADDA
                     AQRRRRDW"
     gene            complement(23064..24092)
                     /locus_tag="VFA02_01745"
     CDS             complement(23064..24092)
                     /locus_tag="VFA02_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU08598.1"
                     /translation="MKDIAKDLGLSVVTISKVLRNHPDIAEETRERVLRRIKELDYQP
                     NIMARSLVTGRSYLIGLIVPDLLHPFFAELAKALSAAIAKHGYSTIISSSEEDPELEA
                     REIQQLTGRRLDALVIASCGTGPQPFLRLDHHELPYVLIDREIPQLSANFVGINDEAA
                     GRLATEHLIDQGCRRIAHVRGRENSTGTRRFEGYKQALQRRGLPFTDSLVVSRSTVDI
                     DSEKMGAEAMRLLLKQRPRPDGVFVYNDPLAIGAMQAILDAGLRIPDDIAVIGCGNLH
                     YDPFLRVPLSSVDQNSQGIGERTAEVLLGILGAKIRPQPRSIILEPSLAIRASSMRKR
                     PARRTAPR"
     gene            24209..25177
                     /locus_tag="VFA02_01750"
     CDS             24209..25177
                     /locus_tag="VFA02_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU08599.1"
                     /translation="MPTRAVVRPGPSMKYRRLGQTDLQVSVLGFGASPLGDVFGRVDA
                     AEGARAVHLAIAEGIHFFDVSPYYGLTLAEERLGQALLGRRDKVVLATKCGRYGAAEF
                     DFSARRVRSSMEESLRRLRTDYVDLFQAHDVEFGDVEQIVHETIPAMRRMQEEGKARY
                     IGISGYPLKVLMRIAESVPVDTILSYCHYNLLNDTMASVLAPFAQKSGIGLINASPLH
                     MGVLTGNGAPQWHPAPPEVHRAVEKARSFCREHNISLPELALQFSLDCTHVASTLVGM
                     STEEEVRQNLRALEKPADTELLRQIKAILAPVANVEWPSGRAENHD"
     gene            25170..26018
                     /locus_tag="VFA02_01755"
     CDS             25170..26018
                     /locus_tag="VFA02_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU08600.1"
                     /translation="MIKRVDAHQHFWAYSPEEYGWIDGNMEEIRRDFLPPHLATEMAG
                     AGISTSIAVQARQTVAETEWLLHLAEEHSWIAGVVGWAPLISPDFPALLERLCAHPKF
                     CGLRHVLQDEPDDDYMLRDGFNRGIRLLNGKDLVYDILIYERHLPIALQFVDRHPNQK
                     FVIDHLAKPRIRAAEISPWRENIREIARRPNVWCKLSGIVTEANWQSWTTDTLRPYVE
                     IALEVFGPQRILAGSDWPVCTLATNYQRWWQTLEAMLSSLSASEQDAVFGGNAIEIYN
                     LKGLKA"
     gene            26015..27040
                     /locus_tag="VFA02_01760"
     CDS             26015..27040
                     /locus_tag="VFA02_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HZU08601.1"
                     /translation="MKALVLRGPGSAQIEDVPPPVRRSGEALLRVRYVGLCGTDLNSF
                     RGRNPLVSYPRIPGHEIAATIAEIDAGAPGLSVGMDVTLSPYTSCGKCASCRNNRNNA
                     CQFNQTLGVQRDGALTEYISVPVEKLCPARLSLRELCLVEPLTVGCHAVARARVTDAD
                     TVAIFGCGGVGLGAVAASAFRGAKTVAIDVDDSKLDIAKKAGAAFAVNGQKQDAKTAL
                     NELTDGLGPQVVIEAVGRPETFLSAVDIVAFTGRVVYIGYAKEPVTYETRLFVQKELD
                     ILGTRNALPQDFREVIAMLEQKRFPVDAAISTIVPMEEAPGVFAAWDAEPSRFTKIMV
                     SCSGDLR"
     gene            27130..28935
                     /locus_tag="VFA02_01765"
     CDS             27130..28935
                     /locus_tag="VFA02_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZU08602.1"
                     /translation="MQRRDFCKLLAATAASSALPTFGQSAAEKSSVLPREFNEYTQDY
                     AEFCALPPEKRTFYELSNGRIVPVKLDNATWKPAEWGRPPELPVAGGSWDGVPMESPI
                     PDLSGDGPYKPTWDSLLQYEAPEWYQDAKFGIWAHWSPQCVPEASDWYARNMYIEGQH
                     QYKYHLEHYGPPSRFGYKDLCAQWTLLNWEPDELITRYKNAGARIFIALANHHDGFDT
                     WNSKHHPWNASAIGPHRDVVGTWATAARRQGLRFGVTVHQARNWWWFQPSHGADSSGP
                     LAGVPYDGILTEAEGKEQWWQGLNPQLLYGVKHPLDALPDISYVRNFYDRTRDLIDQH
                     NPDLLYFDNSLLPLGWGGMNIGAYFYNNSLKQNGGKMEAVLNVKDVPERLVKAVVADF
                     ERGLTSGIMKYPWQSETCIGAWHYARFLYEKPGEYGGYLPPRDVIHWMIDTVSKNGTF
                     ILNIPGRPDGTIDSKEIAVLDGITAWMQTNGEAIYETRPWKVYGEGPNQIKAGSFQGN
                     SISKLGPKDIRFTRNKANTVVYAIALGWPQETIVIQSLGLSAEAKPGKVAKVELLGTG
                     AKVTWKQSADALRIDLPKQYQPKVDYAAALKVTLA"
     gene            complement(29003..>29617)
                     /locus_tag="VFA02_01770"
     CDS             complement(29003..>29617)
                     /locus_tag="VFA02_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08603.1"
                     /translation="IDAQLFNNLDDPMNPRYQYGTAGFDRRNIAVVSFDYNLPVFRHS
                     SGITRTLLGGWTVSGITLMQSGNPLSVNAANDNLGFGGGTTNHADLIGHITYPHKFQQ
                     WFSPTAFAQPAPLTWGNAPKNIVKGPGRDNWNLSLYKDFRFNDRAGFQFRAESFNTWN
                     HTQFTGVNTGVLTGNPSSPYNSTAGQINGTADPRVFQFGGKLYF"
BASE COUNT         6587 a         8561 c         7954 g         6515 t
ORIGIN      
        1 gcaacgtgat ttccgcgtct tttgcgatga tcttcgactg ctccagccca tcggcaaaaa
       61 cattgtccag cgtaacgccg agcttgtgct gggcatccag cccaagaaac gtatatgtgc
      121 ccggagtgag cacatgcaca tttttcagca ggatgtcgcg gtagaccggc agtgcattgc
      181 ctgaaaacgt cgtgtaaagc ggcgtaaaga tgattgggtt gcgcgtgtcc cggatgcaga
      241 cgtcttcata tgtcacgtcc tgcaccagcc cgccgcggct gcggtcagac ttgatgcgga
      301 taccgttgtc ggcgccatct attgtcaggt cctgcaccag aatgttgcgg acgccaccct
      361 ccgtcccgct gccaatggac attccgtgcc ccgtataaaa atggttgtgt agaacagata
      421 tatttgtggc cgcgcccgcc ttgcctgatt tgatggccac attgtcatcg cccgtatgaa
      481 tgtaggagta tgcaatcgtt acgtttgtag atgatccagg atcaatgccg tcagtattgc
      541 gcgcggtctt gggtgtcatg atttttacgc cccatgccgt gaatccattg gtctggttca
      601 ccgctacatg gaaattcggc gagttgcgca atgtaatttt gtacagcgtg aagttgtctg
      661 agtggttgac ctcaatcaaa cggaaacagc tctgcccctt gtcttcaatc ttggcgcggt
      721 gggccaggtc ccaccatgtt tcgtcctgcc cgataagctt ttctccgccg cgcccgtcaa
      781 tcgagccctc acccatcacg ccgctgtcag gagcattgtt caccgcgatc aatggcctgc
      841 acccatgccc tttttcgctc acaattccgc agcttcccgg ggtgatgtca taatcgcgcg
      901 gattgcgcga agcaaagatg gccgtgttcg cgtctaccag cagcgtgacg cccttcttca
      961 gttgcagcgg cccgctcaga aagatgttct tcgcaccgtc cgcacgcagc tccacagcct
     1021 ttcctggttg gcacgaagca atcgcctgct ggatgcgctt cgtgtcctgt tgctgctcat
     1081 cacgctcgct caacttccct gcgttggcct gcaactgcgc cttcagcaca acgcacggcg
     1141 gcggcacaac cggctcagtc acatgccgcg cgtcctgcgc gtaagtggaa aaaccagagg
     1201 taaataaaga tatgaaaaca agactccagc aaaaacgggg catgtatttc tccggaaaaa
     1261 gcactatatc gcatttgata gtggtcagac catagatgac aaccaaaaca catacattgt
     1321 cattctcggc ggagcaccgc acgtgcttag tcggagaacc cgcaccgtga ggtaggctga
     1381 tttgtcctgt gcagcacaga taagataccc ggatgcaggc gggccgtatc ctgtaaatct
     1441 tgacagccat gctccttagt tattaccctt cagtggactg accacatcct cacatgcgaa
     1501 ccagatttct gctcttcttt tttttctgct tattcttcgc cggctcaaca ttcccccaga
     1561 ctgggccaga gatgaagccc tcgctgcctg tcccgcagaa tcctgcacta cctacgctgt
     1621 ttcttatagg cgactccacc gtacgcaacg gaaatggcac cggcgccaat gggctttggg
     1681 gatggggtga cattctgccc gaatacttcg acctcgcgaa gatcaacgta gtcaatcgtg
     1741 cacgcggggg gcgcagcagc cgcaccttca tcacagaagg actctgggat gaaacccttg
     1801 cgctcattaa acccggcgat tacgtcgtca tgcagtttgg ccacaacgac gccagtccac
     1861 tcgatgatgc tgcgcgtgca cgcggaactc tccccggcat tggggatgaa acacgcgaca
     1921 tctggaaccc catcatgaaa aagcaggaga cagtgcacac ctacggctgg tacatgcgca
     1981 agtatgtaaa agatacgctc gccaagggcg caactcccat cctctgctcg cccattccgc
     2041 gcaacaactg gaaagacggc aaggtcgtgc gcaacgaaga ttcttatggc ggatgggcgc
     2101 ggcagattgc caatgaattt cacatcggct tcgtcaatct ggacgaaatc atcgccgaaa
     2161 catacgagaa gatgggggaa gcaaaggtcg caccactttt tcccgcggac catacgcaca
     2221 catcacgcga aggcgccgag atcaatgctg aagcggttgt ctccggcatg aaagcattgc
     2281 cggggaatcc ttttaagaag tacttctctc cgaaagcggc ctctgtaccc aaatggaaag
     2341 gcaactaaca ctctatgaat actgggtgct ttagcagctc tctcatctga accggagctt
     2401 acttaacgcc catacggcgt caccgtaccc acctgcggat ccagcggcag cttcagcgca
     2461 tttgggttgt ccggatacgc cggatcaaac tccgcctcac ccttcacaat tgccgaagcc
     2521 agaggcagtc ctgcctcgcg gatgcccttc accacgcagc gcgccagttc ataagcgcca
     2581 tagtcggtga agtgcgtgtc atctttcagg ggcagcggct ggtcaggaaa tgtattcgcc
     2641 gggaaatgca caaatgcctt cagcgttcct tccggaccca gcgcttcgta caaagtcttg
     2701 ctcattgcgt tcaaatcaat cagtgcaacg ttctgctcct tcgccacttc gcgcattgct
     2761 tccggatagt ccccaagcgt ctgaatgatc ttcccattcg catcgaacgt gcggcggttc
     2821 atcgaagtca ccagcactgg agtcgcaccc ttgctgcggg ccatctgaat gtacttgatc
     2881 aggtattgct tgtaatccga aatcgagaca aagcctttgc ccggcttttg atcgttatgt
     2941 gcgaactgca tgaagagata atcgcctgcg cgcatcgtgc tgaagatttt cgcaaagcgg
     3001 ttttcgaccg tgaagctgtg gatggtctct ccagattcgg cctgatctga aatgctcaca
     3061 gtatcattga aaaataccgg gagcatctgc ccccatgcgg cccacggctc tttgtcctgg
     3121 tctacgacgg tcgaatcgcc cgccagatag agagtaggca catctgcttt ctggatcgtg
     3181 atgctgcgaa acgaaggatt cctgccgata aattccagcg tcaacttgcc gtcccaatcg
     3241 aggctgccca cctcgcgcgg cttcaagtgg acctctccgt cgccctcaat ctgtggcgtg
     3301 cgcacattta cattgaatct gcgcgttgtg gtctggtgcg gcgatgtggc aacagaaagc
     3361 agcatcagcc tgcgcgattc agccttgaca gtggtaacag acgcattggg tccgccaccc
     3421 atctctactg tgacctgata atttccatcc ggcacagtaa gcgaaaagaa gaatggctta
     3481 tctgagaaac acgcgctgtc ttttacatcc ggcaccgtac caggatcaaa gccgaattgc
     3541 ttctctggag aataaacagc atgtaccgta agctcggctg cgcccttctc tttctcgtgc
     3601 tttgtagaag tacaagtaaa ttgccacgac tgcgcggaag caccggagca gcagcaaatc
     3661 aggaatgcaa agacaatgga aacgcagaac agtttttggg gtagcatcat gcgtggtatc
     3721 tcaggcaaaa atttgtcatc atgcggcagt agacaaggac ttgctttttc catgcacagg
     3781 tccttcactt cgctcaggat gacaacctgg ttagagcggc actcaacgct tcgtcatgcg
     3841 cgccacttct gaccccgcaa gcagaaacgc ccccactccg tagtcatagc tggcagttgg
     3901 tctgtaccag gcaggagcag cgccggtctg ctgaatgcag ccaagccggc catcggcgta
     3961 gatgtgctcc agcattccct tccacgcgcg cgccacaacc ggcttataca cctttcggtc
     4021 gagaatgcct tcattcacgc cccaggccat cgcataggta aagagcgcgg agccagaaat
     4081 ctccggcagc ggataatccg ccgggtccag caatgacgag tgccacagcc cgtctttcgg
     4141 gtcctgcagc gatttcactc tatccgccat ctcgcggagt aactcgacat acttctcgcg
     4201 ccgcgcatca ttcttcggca tatattcgag cacgcgaacg atgccgccca tcacccatcc
     4261 atttccccgt gaccagaaaa tcttttgtcc gttcttctcg cgcttgtcca gatacgtggc
     4321 gtcgcgcgca aacagatgtt cctgcttgtc atagagcagc gactgtgtct cgtcccagtg
     4381 tttctcaatg tagtcgagat atttgccgtc gttcgtggcc gcatacatgc gtgcccacac
     4441 cggaggcgcc ataaacagcg catcgcacca ccaccactgg atccgcccct gcttgtcctt
     4501 ttccagcgga tacagcgcgt cgagttccgc gcgtgtgggc gcaatctcag ccggatcgcg
     4561 gtctatgaaa taaagctcca gatatgactg gcccacgctc tggtcatcgg cattcggcag
     4621 atgcgagcgc agctcccagt tgtacttctt tcccacttcg cgcacagcgt cgcgataacg
     4681 cgaatcgccc gtggaggcag atgcagccat caagccggta tacagcacgc ttgaggtcca
     4741 gatgcggtca aaccagggct gccaggtctt cagctcccaa tcggcgactt tggtcatggc
     4801 cttgtgaatg tccttcacct tgagcgcagg cgaaagatct tttgccatag gaccaccatc
     4861 taccggatca tcgccgaagt ggcgagcatt gtctttatca atgcctgcac gctggtctgg
     4921 cgtcggctgc ggatactggg cctgcgcaaa acatggcacg aatgagaaga acgcaagcag
     4981 aatagggaca atcgtaattt tccactttct tacgcaaagg cggggaaaaa gagtggggta
     5041 cgcgaatatg gcgtgcttca ggaaactact ttccatgccc cggctccttt gccacttcca
     5101 cggcgtagac ctgctccgca cccgacatat tcgagcgaaa gacaatccac ttgccatcgg
     5161 gagtaaagtt cacatttggc tccagcgcgt aatcgtgctt tgacatgttc accagcttct
     5221 ccgcctcgaa tgctcctgct tcaatcaggt ctttttcctg cacccccgtg tttgggaccg
     5281 gttttggatg aaacagatag atccacttgc cgtcaggagc atgcgccacc atcttactgt
     5341 ctccaccgtc gcctgcaaaa agagtcccgt caggcgagac gttgaagtgc acggaccact
     5401 cattgcgctg caggtgccac catgtacggt gtccgtcata gacgttatat ccgcccaccc
     5461 agaagtcttc tccgcgaggc gtctgcaagt cataccagat ggtcttgccg tccgctgacc
     5521 agaactcgtg cccggcaatt tccatctgca tacggcgctt atggacgagc atcaacccgg
     5581 aaccatccgt atggatcagc cagatgcggt caaccttctg ccatggccct tcatggcaga
     5641 acatcagcag gttcgggtcc gtcggcgaga agagaagatg gttcagccat tccgtgctgc
     5701 gattcagtac tttcacttcg cctgttttga tgttcagtgt gaagagcgcc atgggcagat
     5761 gagcattgta gcgttcttcc agcatgtgcc cccggccatc gcgtgtctgc ggcggcggtg
     5821 aaagatgctg cgctttttcg attccctgcg attcaacgta cgtgcccgcc agcagcgttt
     5881 cgtctgcatt gacagtggcg atgctgccgt gtggcggcat gtcggcaacc tttgtctctt
     5941 tcttcgtctc cggatccaga taccacactg cgccatcgcg cgcaaagtat acgcgcccgg
     6001 tcttgtgtcc caccatgatg gggtgcgcat gaggatcatg gttcagtggt tcaatcctgc
     6061 gtgtggccag gtccacgaac gccatcccat gcggcgtcgt aatcagtagc ttatcgccgt
     6121 tcggagtgta tccgttgaga ttgaaataaa ggctgccgct gcccggttct tccgaaagct
     6181 ggacaatgcg gtgccctgtg tccgggtcta cccactcttt gggcgtttgt tgcgccagaa
     6241 cagcaaacga tgaagacacc agagcaagaa cagcagagca ttttgccagg aacatagaag
     6301 ttttcataaa agtttttctc agaccaggga aaagccgagg atatgctttt cacaacggtt
     6361 gcatcacgat ccatttccgc agcgcacatc ctctctccag caaacaccag cgccgaaggc
     6421 gcgcccgcct ggacggccag tcccaaaaaa gtgcggggcc tccgatgtcc tgaaaggccc
     6481 cgcgtattag cgggaggcta aaaattgatc cgtcctgaaa actgccagtc gcggttgcct
     6541 cttgccgaac cgacggtccc gaatgtcgtc gaacccggtc cccaggctgt actgattcca
     6601 cccttagtgt tgtggttgaa cacatccgag cagtcggcgc gcaccacaag ctgccagcgt
     6661 tccgtcaact ggaaggcccg ttgcattgtt gcgtccatgt tccagtcgct cgggccccac
     6721 agcccccatg gtgctgtacg cggtgcatcg ccaattttgt agatgggtgt cttcgtcgca
     6781 tttccaaagg ttgcaggagt gctgaacgcg ttcacatcaa tgtactggat ggtcgagccg
     6841 agctgtgccg cagtaatgcc tttgccccag ctcccgtttt tgcgcgccga gccgcgaccg
     6901 aagaaagcag gattaacgtc cggcatacac tggccttgcc ccggagcagt gcagccgccc
     6961 catgtaatcg ccagcggcgt gcctgaatga taggtataga tgctcgataa ctgccacccg
     7021 cctgccagcg cgcgcaccag tgcgctgtca ttgccaatgc cacctttgcc gaacggaagc
     7081 tgatacaccc cgaacgctga cagcgtctgc ggcatggaaa gcgtcgtata agaccgctct
     7141 attgcatcca ttttgtatga ccttccgttt gacatcgccg cacccggaat ggcaaagccg
     7201 ctgcggaagg tgccgtcatc gcccaggttt ttcgagtagg tgtaattcac catatagctc
     7261 agtccatgcc acggtctttg ctggagcgaa aactggaacg agttgtagct gatgttcgcg
     7321 ctgttctgcc cccaggtgtc gcttactccg ctgtactgag gaaatgcgcg cagaacagtc
     7381 gcaatcgtcg ccttcgtgtt gttgttgacg aatacggaag gaatattcag tcccggcatt
     7441 gcctgtttag caatggcgac atttgccgga gtcgctgcat cattcaggat tgggttcccc
     7501 ttcgcatccg ccacacctcc cagcgcggcc agataggccg gatcgagctg gttcgaccag
     7561 tatccacgaa tattcgagct gccagggact acaaaatggc tttgcgtgcc tacgtagttc
     7621 gcgcttgcca ccaacgtatc ggtaatggcg cgttgaatac cgaaactgta aaagatgaat
     7681 tcaggcgcgc gcccggaaag ataggggtcc gcatacccag gggccgagct tgccgttaca
     7741 aatttccccg tagaggggtc cacatagttc cctgtgctca aagtcaggct cgctgtgcct
     7801 ggcgctgaag gcgtaggcaa tgcgaacccc gggccaccaa acgctgtatt cgccagcccg
     7861 atagactgaa aatacggact attgttcaga taaaacgatg gtccggcacc tgaacccgtt
     7921 gtaagctcta tcggggcggt tgcgttcacc gtgaatccca gctgtccggt accattgcct
     7981 gcacctgcgc gtccgcccac gccgccggca tgggaataag ctatggcaaa ccctccgcgc
     8041 accacagact tcggatccgg ctgatacgca aagccaatac gcggcccgaa gttcttccag
     8101 tacgtgtgca ccggagtccg gcaattgcaa ctaatgtccg caccgcgatg tccggcaaat
     8161 tccagaacac caggcgtgcc cgtggctgga ttgatgcccg tcggattcag gaagctccag
     8221 cgatccagca cttctttaaa cggtgggaaa tagtcccagc gtagtcccag atttaccgtg
     8281 aggttttttg atatcttcca gtcgtcctgg aaataagggg aaatcggatg atagcgtccg
     8341 cctgtttcgc tgaaaggctg gattgcgatg cttggcgaac cacccactgc ccccagcaga
     8401 tagctcgcgt aagaataccc tgaggtgttc gtggccaggc tcgtcgagcc tggggcaaag
     8461 ttcgccgtgg aatttgcatt gtagcccatc gtcaggattc ccgaaggacc ggccaacggc
     8521 gtcaggttgt cctgcagcca ttgcatctgc accccgaagg tcatcgaatg gttgcccttt
     8581 acccattgca ggttgtccac cagagtaaag gcgtttggta ccgtattctg cgtcgcaccc
     8641 gacgcgccat tggccgtcca cgccgactgt accagaggaa agagagtgct cgcgctgaag
     8701 ctcgccccgg gaaattccgt tgaggcctgc ccctgcggca ggttcgtaat gccggccgtc
     8761 acagcttccc actgtttcac tccatccgta atcgccgtca ctggttgcgc aaagcgcgtg
     8821 tatccaaact tgaactgatt caccaggtat ggcgtgatca cgatagagtg ctcaatgtcc
     8881 gcaaacaccg gcttgatgat cgccttgccg ccatttgtgt aaggaggggg cagcaccacg
     8941 ccactgttgc ccaccgtgaa cggaacgttc tcgcggtagc catatgcaaa cactcccgag
     9001 agcttctggc tgcccgtcag gttataatcc accctcgacg tgatctccca gttgtcatat
     9061 cccgaaggca cgccacccag atagttgttc gtgatctgcg agttggttgg ctcaggtaag
     9121 aacttctgca tgtactgcgc gatgggcgaa agataagact ggggaatcac attcggcgac
     9181 cccgttggca cgggatttcc attcggcccc gggatgccgc catatccata tccaaactgg
     9241 tagcggcacg gcccgttcgt gctgtgcgcc gtgcaggtgg cctccgatgt tggatcgtaa
     9301 atctgccccg tcgtaatgcc attcgtggtc gtcagcagtt cactgaagtc accctgccgc
     9361 atcttcattg ttggcacggt caacgtgttc gggttgacac ccgagcggcc gtgataacgg
     9421 tcgtaagcca cgaaaaagaa gcctttattt tttgtgaatg gaatcggtcc acccactgcg
     9481 cctaccagct cattctgatg ctcatctggc ttgggagcgg gcactgtctg tcccgctgca
     9541 tttttcactg tcagcgcttt ggcagagaag ccccaggtat caaacatgcg cgcgcgcaca
     9601 aagtccgcca ctgttccgtg atactgattt ccaccagact ttaccgtaaa gttcattaac
     9661 ccggcaccct ggtactctgc acccggaaca cttgttacta cctggaactg gtctactgcc
     9721 tccacaggaa cagcattgaa gatcacgcgg ttgtcaccct gctggttgat cgtcgttgtc
     9781 gggatgccat ccagatagac ctccgccaga tagtttcccg tcccaccgat caccggcgcg
     9841 cgcgtgcctc cctgcgtacc cggcatcaga gtggcaaatg cagtgggatc gcgttgctgc
     9901 ccgcccatca aaatggggag gttctggtac tccctgtttt ccatgacacc gcccaaggtc
     9961 gcggtcgtgg tctctaaagc cggaggcgcc gctgtcactg tcacctgttg cgtctgcgat
    10021 cccagactca atttcacatc cagcggcgtc acagtcagtg cgttcaccgt caggttttct
    10081 tgtttaaaag actggaagcc agccgcagtt accgttaccg tgtaggtccc aggcagtacg
    10141 ggcgatatca catacaaccc atctgaagaa gtggtgcgcg tatattgcgt tccgctggcc
    10201 gtattgaccg ccgttaccgt cgcattcgga accaccgcgc cgctcgggtc tgtgaccgtt
    10261 cctcgaatcg cgccctcacc tgccgtctgc gcccagccgg aaacgctaaa gcacgcaact
    10321 gccatcaacc ataagaaaag catccactgg tcagaccgta acccggagaa cttcaagctc
    10381 ttattcgacc tcatacacca gcctcgaaat gaatccgcgc agaccgtctg ttgtctccat
    10441 ctttgttccg gcccggcttt gaattgaatt gactgcgaaa aagctacgtg cccgtgaaaa
    10501 cgattgtcaa taagaaaaca agttctcatt tggtgtagag tgaaaaagca ttcaaaaaag
    10561 aggccgattg taccgctatg tggtacgacc tctgcatgga aagagagaac gtcgtctcca
    10621 tcctctcgca tgtcgaaagg atagagacca cgattgcaaa ctatggagct caagcgggct
    10681 gctttattta gatacaggga ctccaccctg tggatgattc tccttacgct tgttctttct
    10741 gcttctgccg agcgccgcat ctctgtcaac gattacggcg caaaaggcga cggcatcgct
    10801 cttgacacgc acgccattca gtcggccatt gatgcggctt ccaaggccgg cggaggcacc
    10861 gtcacctttc gccccggcac atacatctcc ggttccatct ttctgaggtc gcacgtgcat
    10921 cttgaagtcg gaaacggtgt gatcatccgc ggtattcagg acacaaacca gtacccgatg
    10981 atgcccaccc gcgtagccgg aatcgaaatg acctggcccg ccgcgctcgt gaatgtctac
    11041 aagcaggaag acgtaagcat cagcggcccg ggcaccgtgg atggcaacgg caaatactgg
    11101 tgggacgcct actgggcctt gcgccatgaa tacgagccga aaggcctgcg ctgggcggcc
    11161 gactacgatg ccaaacgacc gcgccttatc cagatttttg attcaaaaaa ggtcagcctg
    11221 aagaacattc gtctggagcg ctccggcttc tggaccgtcc acatctgcta ctcgaatgac
    11281 gtaacactag cgggcgtcac catccgcaat aatatcggcg gcaaaggccc cagcaccgat
    11341 ggcatagata ttgattcctc gcgtcacgtc cttgtcgagc acgccgatat cgaagtcaac
    11401 gacgatgccc tctgcctcaa agccggacgc gactgggatg gcctccgcgt ccataggccg
    11461 accgaagaca tcgtcatccg cgactcaacc gtccgcgccg gggccgccgg attcaccatc
    11521 ggtagcgaga cctccggcgg ctttcgcaat gtggatgtct accgtcttac agtgcttgcc
    11581 cccgtacctt cgggaatcct cttcaaatca gaccgcacgc gcggaggagg cgctgaggac
    11641 atccgcattc acgacatcac gatggatggc gttgccatcc ccatccactt cactatgaac
    11701 tggaacccga aatatagtta cgccacgatt cccgccggca tcaccaatcc accagattac
    11761 tggaaaacca tcacgcatcc ggtcgaaccg cctgagcgtg gcctgccgca cttccgcgat
    11821 gtaaagatat ggaacatccg cgccactggg gcaaaaaagg catttgtcgt aagcgcctac
    11881 ccggaagcgc ctttaacgga cttcgatttt tcgcacatag acatcagcgc gcaaaatgca
    11941 ggctccatta ccgatgcaaa gaactggaaa tggaaggacg taaaaatcca gacagaggac
    12001 ggcagtaaac tgaagctgcc ggaatagctt ttctgcattc cgtgtcttca ggatttgtga
    12061 catttaccgt cgcgtcttcg caatctccgg cctgagcgcc atctcgtcgt ctgcctccgc
    12121 agcctgctct tccggcagaa gatggttctt cgtcagccca tcctttaata tctggaacgc
    12181 ctcttcagga gtatccgcaa actggaacag ttctacatcg cgcggcgaga tggccccctt
    12241 ctctaccagc acgtcaaagt tcagcacctt cttccagtaa gccgaaccgt agacgacaat
    12301 cgtcatcttc ttggccagct tttgcgtctg cgccagcgtc aacagctcaa acatttcatc
    12361 gagtgtgcca aagcctccgg gaaagaccac cagggctttc gccagatagg caaaccagta
    12421 cttgcgcatg aagaagtaat gaaactcaaa gttcagcgca ggtgtgatat agcggttcgg
    12481 ctgctgctca aacggcagct tgatattcag cccgatggtc ttgcctccgg cttcatatgc
    12541 gccgcggttg gccgcctcca taatgcctgg gccgccgccc gaagtcacca caaagcggtg
    12601 tcgccgcgac ttcaacccct tggcccagtc cgtcagcaga tacgccagcc gccgcgcatc
    12661 ctcatagtag tgcgccatct ccacagcggc ctcggcgcgg cgaagctgaa gctccgttac
    12721 tgcgccgtcc gttgcaggct gctcttcctg cggggctgga cgctgcgagc cggtattttc
    12781 cagcagctcc agttgatggt tcgcctcatc gagcgcgcga aaccgcgccg acccgaagaa
    12841 gacaaccgtg tcctggatgc gctcgcgccg gaagcgcgcc agcggctcca gatattcaga
    12901 aaggatgcgg acaatccgcc cgtccgggct gttcagaaaa gccgggttgt cataggcaag
    12961 gggagcgctg gcaagctgtt ctgggtcgag ttcagacata aatcaaggct cacgataatg
    13021 gatggcgtcg gtgatggcaa tccagatgtt cagcagcccc agtccggaca ccacaccgcg
    13081 cactgctcca ttcgatgcca gccacgcgac ctgcgaaaca aggttgaaca gatggttttc
    13141 cgtccagaga tgtgtccagg gcagcgccac aatcagcagt ccgagataca accgcactac
    13201 gacgcgcaga aacagatcta tgcggcgaag ccacacaggt gtgcggtgct cttcaggaat
    13261 tgcgggtttg ctctcaggac cagccgggcc atgcggagtg ttcgacgaaa acaggttcgg
    13321 ctgatacgac atgcgtcttt tttcaagcta tcacagccgt ctcccgagtc tcacacctgg
    13381 cgcctgcatc tcttcaagaa atgtggacca aatgcaggct tgttacaatt ttttctgcct
    13441 cactgtcgtg ctatgataac ggggttcggg aactgtccct gatatatctt tctcagtaaa
    13501 ggagcaccca tgcccccggc ctttgctggt aatattcagg tccccacggc acctgtagac
    13561 cattcctcag accacaaact ccccaacctg cgcagcctga atccgtcggc catccatact
    13621 accgctgcgc taggcggcac gattggccct catgtgacgc tggtgcatgg tgatgaatgg
    13681 accatcatta caggttcaga gaagcggaca atccagaacg accagaccat cgagattaaa
    13741 ggcaacgagg ctcataccac cacgaagaac ctgacctata aaatcattgg ccaaacagtg
    13801 gacacccgca ttggcgcaca ctatcagaca aacaattctc cccggtttga gacctacgtg
    13861 cacactctga ctcaggacca tcacgaaaag atgattgtgc accagccaac aacgctcatg
    13921 gacattatca acttatattt cggtcgaaag tgggtttcgc tgaatgcgac tggggcgagt
    13981 ttcagcgcaa caggtacttc tgcttctctg acctcgctta gtggttccgg aaccgtaatg
    14041 agtgcttcaa acacggtctt ttcatccagc agagaatctt tctctataaa ggaccttgat
    14101 attggtttac atgtacgcgg aatgatcacg gagttgaaag ccatgaagct taaagcggca
    14161 ctcgcccacc ttaaggcaat tattggaaac ttcaatgctg gcattgctgc aaatatgagc
    14221 agtccgttca gttaaatatt tgccttcgta caatatgagt tctgcgggga cttacatgct
    14281 tggattcagc gtccatgtcc tgaatatggg agacaaactg acgattgccg tgccggcaga
    14341 cctgcctttg ttcatcattt tcttacttcc actgctcatt ctttttggcg tttaccggga
    14401 gatgaatacg tcccgtggcc gcaaaaaatg cgttgtctac atgctcatca acattgtcat
    14461 cctgagtatg attttcaccc ccgcaaaagt agaactggac gccaggcaag gaacagccac
    14521 cgtacgaacc gtaatgtttt ttgttccccg gcatcatgtt attccactct cctctgttca
    14581 aggtgcaatg gtaaaaacat cagagctaac cgaggccttg gcgcttgttt tgactgatgg
    14641 acacgcgatg caactcactc cttttgacca ggtacgtggt acggatgtcg cagctgcggc
    14701 catcaatgac tttctgcgca cacacggagg agtggggaaa ccatactgaa atacacacat
    14761 gccaattagt cttacggatt cctgctgatg atggacatgt ccctccgtaa aaaacgtagc
    14821 cgcattgatc ccattcggcc agtaacggat ctctctcctt ttctgagagc agcgcagatc
    14881 accgacttct cgctttactg ggcacctgtc acggagtacc cagtcccgct gaaccagcgc
    14941 gaatggatgc gcgaattctt aaaacgtctg gcggggcatc tgaaacagaa agacgaatta
    15001 cgcgaagaat ggttcctaca gttcaaggcg ctctatccac atctgattga ccgctttgtc
    15061 gaatgcgcca gccactatgt tccggtgacg ggactgggat ttgtacccgg cgctgttcct
    15121 gccgaattac ctgattttga ggctgtaaaa aaagaagtag agaaggccag taaagacgga
    15181 agacacgtcg aagtacagca gtggatgcca gaccatgtgc actggtttgc cgccaaacaa
    15241 ccggaagaac aacgccggga cttctttgga catggtggca tgttcgcgct ctacatgaga
    15301 ccaaacacaa aagcagctcg tgtcttccct caactgccgc ggtttatgac cacccatgaa
    15361 gcctacactc ccgacctgga gcgacagttt gccatgctga attctttaca agatgatttt
    15421 cttgcgaaaa gcaaggagat cttcggcacg ccttttcgtc aaaagcccgg ttatcgtgga
    15481 ttggcattca tagtgccact gctttccagc agaagtttcc ttgactctac cccagagcaa
    15541 cgcggcactt ggtttcgctt gtttcaggga tatttcatcg aaagcagaca ggacaatggt
    15601 gttctgtttg catccagtga ttttgaattt gatgaagatt tgatctcgat tctggaagga
    15661 atgcggagtg acgggttttt ctatcgcaca tagcacgaaa aaaaactgga aggttcgcgt
    15721 ttttccatcg gaaggtccct ggcactatgc cagcctcatg gttcccgggt tcccactcaa
    15781 gtcccctctc agggaagatc tgcggagtac tctctggaca gccatcgagg cggtacgtgg
    15841 cgcgcagata ttgcagcttt cagtatggct attaatggat tctctgcata aagacggtgc
    15901 cgtccatgct ctaagcaggc atggtgcgga gtggttgcca gacttcggct tcggcctttg
    15961 gcctgatcgc gagccgcccc gcatgtatga agaaagagat ttccttgatc tggaatttgg
    16021 tgcggaaatg cccagattga tgcactcagt cgcccatatc acaacagaga accaagatac
    16081 cgtaactcac gcatgggaaa ctatgttcgg cactggtagc actgttcttg ccctttcaag
    16141 cgtttccgca gacaacattc tggccaccat gcgggtagcc gcacttgaaa aaattcagga
    16201 tgaaaccttt cgcagttttc catggtattt ttctctgatt ggacagaagg cgatggaagc
    16261 aggtccagtc ttgcttgatt cgtggctcgg cccagtcgag ctttatgtcc gcgagagcga
    16321 agaagatggt tccatcgtct tgctgggccg ttctgatttc agtctggcgc tagaagccgc
    16381 aggtttccac ctgatagaag aacctccgca aaagtaaacg catatcatgc cggaacggca
    16441 ttccgcttcg gcctgcggtt ggcctgcaaa actttcttgc gcagacgcag cgacttcggc
    16501 gtcacctcga ccagctcatc atcggcgata aactcgatcg cctgctccag attcagcagg
    16561 cgcggcggcg ccagacggat ggcctcgtcg gcgcttgatg cacgcatgtt agtcagtttc
    16621 ttctcgcgca cggcgtttac gtcgaggtca ttgtcgcgcg agtgctcgcc gataatcatg
    16681 ccttcgtatg tctccacgcc gggctggacg aagagcacgc cgcggtcctg caagccatcc
    16741 agtgcatagg cggtgatggt gcctgcgcgg tctgagatga gcgctccggt actgcgctgc
    16801 ggaatctcgc cctggtaggg catgtatccg tcaaagagcg aattcatcac gatggtgccg
    16861 cgcgtttccg tcagcaactc gctgcgcagg ccaatcagcc cacggctggg cacgcggaac
    16921 tccagacgga cgcgccccga gccgtgattg tgcatcttca ccatctctcc cttgcgtggc
    16981 ccaaggcgct caatcactgt tccgacaaag ttttctggaa tatcaatggt cagatgttca
    17041 accggctcca tcaccttgcc gtcaatggtg cgcgttacaa tctccggacg gcccaccatc
    17101 agctcgtagc cttcgcggcg catcatctca atcaggatgg cgagctgcaa ctcgccgcgc
    17161 cccagcacct tgaaggcatc cgggctgtcc gtgtcttcca cgcggatgga aacattggtc
    17221 agcagttctt tttccaggcg ctcgcgcaga ttgcgagagg tcacaaactg gccctcgcgt
    17281 cctgcaaagg gcgagttgtt caccatgaac tgcatcgcaa tcgtcggctc gtcaatcacg
    17341 atgggcggca gcggcgcagg gttttcgatt ccggtaatgg tctcgccgat ggtaatgcct
    17401 tccacacctg caattgcaac aatgtcgccg atctcagtgg tttctatgtc cacgcgcttc
    17461 aatccggcaa agctgaagag cttggtgatg cgcgtctttt gcagggcgcc gtcgcgtttt
    17521 gaaatgtaaa cttcctcgcc ggtcttcagc gttccgttaa agacgcgggc gatagcgatg
    17581 cggcccaggt agtccgaata atcgaggttc gtcaccagaa tctgcagcgg cccgtttgca
    17641 tcgccgttcg gcggaggcat ggtcttcaga atggtctcaa acagcggctg caaatctttg
    17701 cctgggaccg ctgcgtctgt gcttgccagg ccgagcttgc cgttcgtata aagaacgggg
    17761 aagtcgagct ggtcttcggt cgcatccaga tcaataaaga gatcgtagac ctcgttcagc
    17821 acctcctgcg gacgcgcatc agggcggtca atcttgttga ttaccagcac cggagtcagc
    17881 ttcgcttcaa gcgcctttga gagcacgtac cgcgtctgcg gcagcgggcc ttctgaagcg
    17941 tctacgagca gcaccacgcc gtccaccatt ttgagtgcgc gctccacctc gccgccaaaa
    18001 tcggcgtgcc cgggcgtgtc cacgatattg atcttcgtgt ctttgtagta gatggccgtg
    18061 ttcttggcca ggatcgtaat gccgcgctcg cgctccaggt cgttcgagtc catcacgcgt
    18121 tcggccacgg cctcattggc gcgaaaggtc ccggactggc gcagcatagc gtccacaagc
    18181 gtggtcttgc catggtccac atgggcgatg atggcgatat tgcgaatgct cgtgctcatg
    18241 gacacagatt tcctcttctc tacaaataaa gcttcaggaa tgtctcggaa tgccgtcagg
    18301 caggcaggtc agccgaaaat gtttcctcta tccagtttac ggcatgcaga ggacagagcg
    18361 ttcactgagt tgagatgtca gatcatccag cctgactgcg aaccggaata ccgggcccct
    18421 tacttcaatc ccctcttcat ccggtacaca atccctactg aaatcgcaaa ctggtcctgg
    18481 atgccaccgg agccgaattt tgtaattgtt gcgtccggcg agatgcggaa gaccagcctc
    18541 ggcgagcggt tcaggtcgag tgatccgcca aaggccccgc caaaagtaag ctggttgctg
    18601 aacaagccca gctctgcagg gggaacattc ccgatatcgt gctggaaatt accataggcg
    18661 ccacccacca gcgcgtgcag agtcagcgag gcatgttcat tgctgggtcc gcgccattcc
    18721 ggaccagcca gaaacatatg ctcacttaca aatggtcctt tgatgttgta cacgttcgga
    18781 acgacgccgc tggtgccata atatccgcga acattggcag ccgccgccca gcgtctggta
    18841 aagaaccgcg ccgcctgcac atcaaatccg cccaggttcg cgccttgcag caggtttggc
    18901 cctgcattaa agtgcgagta cgcgaaacct ccgtaaagct cccacttgta gtcgtaatgc
    18961 actccggcga gagcgtagtt gggagcaacc tgcggctgct ggttaggaga cgaaacggta
    19021 ccctgcgtct gggcagaagc agcggaaacc atggcggcaa gggccagaaa agaaaaaagc
    19081 gttctcttaa acaaaaaact cctccagaca tccggcgaac agaaattttg ccgtaaattc
    19141 taaagataca tggcagaatg gccttctgtc agatagacgg gcaccacccg tttcgggaag
    19201 ccctgccgcc gcacctccgc atcccacttg ctatactgag taggaatgca ttgtttgcat
    19261 tcaatttccg gaggaaaccc atcgcacaat tagacaaacg ttcggccaag cagtttatcc
    19321 gcatcaatga aagaatccgc gccagagaaa tccgcgtgat tgatgaaaac ggcgaacagc
    19381 tcggcatcct gcagcccttt gaagctttga aaatcgcccg cgagcgcggc cttgacctgg
    19441 tagaagtctc gcccaatgct gtcccgcccg tctgccggat tcaggactac ggcaaatatc
    19501 tctatgaaaa agacaagagc gagcgcgccg cgcgcaagaa gcagaaggtc attacggtaa
    19561 aggaagtaaa gttctcggtc accgtagatg agcacgacta tcagaccaag aagaaccagg
    19621 ccatccgctt cctccacgaa ggcgacaagg tgaaggcttc cctgcgcttc aaaggccgcc
    19681 agatggccca ccgcgagttg ggctataaca tcatcaaccg gctcattcag gatgtaggcg
    19741 acgccggaac ggtcgagttc atgccgcgca tggaaggcac cacgctccat gccattctcg
    19801 ccccagccaa aaaggaagcc ccgaaaaagg ctgctccgcc caagcctcct gcgccgcaaa
    19861 cagcgccggc cgcacaaaca caggcatagc ttttcccatg caaacccgat ctccggaacg
    19921 cgaggctttt gcgttccgga tggaagcctc cccgtctttg aacagctgat gccgggccat
    19981 atcctgtccc gctgccgctg cgcgatatcg tcagatgcca agtaatggcc gccccgggca
    20041 gcccgatgca aaaccagcat caggaaccat acaggtccca ccacgctccg ccgctgcact
    20101 gatggagcag tagtccatcc gcagttccac ttttgcggga aaataagcat atgccggata
    20161 caacaggaac gctgaaaacc cacaccgaat acctcttttt tcacaccagg aagcgtcgcg
    20221 aaatcgtcca cattacccct caactggaga aaattgtggt gcgcagtggc atccgcgacg
    20281 gcttttgttt tgtctcacct atgcacatca ccgcggcgat ttacgtcaat gaccacgaaa
    20341 gcggtctgat tgccgacatc gaagaatggc tggaaacact ggcgcccgcg cgtcccgact
    20401 accgccatca tcagactggg gaagacaacg ccgatgcgca ccttaagtcc ttgctgctgc
    20461 atcatgaagt cacgctgccc atcacggatg gccggctgga cctgggcaca tggcagcgcg
    20521 ccttctacgc ggaatttgac ggccaacgcc gcaagcgcgt cattgtgaag ctgatgggag
    20581 tggcttagtc agcgcagcgt tgaccgcctc aggtcttgcc tgagaaacat tcatggccgg
    20641 cgcacgcgga atggtcatgc tgggcagaac gataatgtcc acccggcggt tctgcgagcg
    20701 gccatccggc gtggcattgt ttgctaccgg atggaactca gcataccccg acgccgaaag
    20761 ccttacggga gaaaaatggt gtgccacaat aaacattttc gccagccgcg tggcgcgcgc
    20821 agtggaaagc tcccagttgg aatcgaactg gtcattgtga ataggcacat tgtccgtatg
    20881 cccctcaatg cgaatgtcat atggagtcgg gcgcagcgca tcggcaattg catctagggt
    20941 cctgatggag tccggatgag gcgtagcaga gccgctgtca taaaagcctg cctctcgcag
    21001 agagatgacc aacccgtcgc gtccaatccg gagggcgacc acatgatgcg caatctggtt
    21061 tgagagcagt ttttccagtt gctttttgat gcgctcgagg tcctgccgca cctcggggga
    21121 agccagcatc tgctcgccta atacaacatt gaccggcgaa acctgcgcat cgggcgacgc
    21181 agagagcgca gcggcctttg gcgtctggtc tttgctcacc gattgaaaca gtccaagcga
    21241 tttgaacgca tcattaatgg cctgtgccac tgctgcctgc ttcttcatgt ctgccttgga
    21301 actggcatac atgaccacaa agaaggcgaa catcagcgtg atgaaatcgg cataggaaac
    21361 cagccatcgt tcatggttcg gtgcgtggcc cttttgtcct ttctgtttca tgcggcctcc
    21421 gcttctacag gcttggcaga cttccccttt ttcccggccg caggatgaaa cccctgcagc
    21481 ttgatctcaa tcatgcgcgg attcatcccc tccagaatcg agatcacgcc ttccagcacc
    21541 atttccttca ggcggcgctc atcggcgacg cgatagcgca gctttcccgc cgcaggaaga
    21601 aaaaagatgt ttgcgctgcc gacgccgtaa atggtcgcaa caaaagcaac cgcgatgccc
    21661 ctgcccacct catccatgtg gtccaggtgg cccatgacct gaatcagccc catgaccgcg
    21721 ccaatgattc ctatggtcgg agcaaagcct cctgccgact cgaagacggc aggaatcgtg
    21781 tcttccatct gggcctcgtt ttcaaggtgc aactccataa tcttgcgcag ttcagccggc
    21841 tcggtgccat ccacagccag catcagcgcc tgcttcataa acgggtcggc aatagaggcg
    21901 aggtcctgat cgagggagac aatcccgctg cgccgcgctt tgttggcaaa accaagcagc
    21961 agctttacgg tggcgtcggc gtcgttcttt ttttcaaaaa agacctgcgc cagacgcttg
    22021 aatgccgagc ccaccgtcgc cagaggaaac tgaagcatga cggcgcccat cgtcccaccg
    22081 agaacgatca tggcggccgt cggctgcagg acctgcccga tgtgtccacc ttcaatcaat
    22141 agcccggcca ggattccggc cagcgcaagc accactccag taaagctgct cttgtccatg
    22201 gatttgccct gaaatttgga tttaccagtc cctgcggcgc cgttgcgctg catcatccgc
    22261 tccggggacc tcgtccatag cggaatttgc tgcaacaatg cgcagtcccg tggatttggc
    22321 cgaagccgct gcaaccgcgc ccaggtcgtc agtcgtggcc tgcttcatcg cgtcggccag
    22381 cagttttgtc cggtaggcgg taatgcgccg cacaacctcc gcgcacggtt cggccacaac
    22441 gatcttttcg ccatgaagca acgtgagggt cgtgtccggg gacgactcga catacttgat
    22501 cagatcgctg ttgacgacca tcggagaccc gttgaggcgc gtaagttcaa tcatgtatgg
    22561 atgacctctc gattacagag aggcttatcg gcaggagcag gaaaaggttc aaggaacgga
    22621 aggatgcgtg gtccggaggg gccatccata gattgtctat ggataaggag ggaaatcagc
    22681 gcgatttttt ctaaattcct gaaaacaaaa gcttaaaatg atgcgttctt tcttattttt
    22741 gcgcgatttg atatctggat tgtctgctta tgccaggccc gagtccaaaa atggagtgta
    22801 caggacctgt gaaaatgcct ttaaaccttc atatcgctaa gattcttccc cttcgatcct
    22861 agaatgacgg gactctccgc acaatcggtc tctgctgcgc gggcaagatg cggatttttc
    22921 cttctttctt tcaggatatc aggtggaagg gcgctctccg gccttgcggg caccagtata
    22981 tattcctgaa aacaggaagt ttagacagga gacgttacct ttgtaaggat tgacagagat
    23041 ggaatgcgag cgggcatcat cttttatcgc ggagcagtac gccgcgccgg gcgttttctc
    23101 atactcgatg cgcgtatggc gagcgaaggc tcaagaatga tgctcctcgg ttgaggccgg
    23161 attttggccc ccagaatgcc gagaagcacc tcggctgtgc gctctccaat cccctgactg
    23221 ttctggtcta cgctcgacag cggaacgcgc agaaaaggat cgtagtgaag gtttccgcat
    23281 ccgatcaccg caatgtcatc gggaatgcgc agcccggcat ccagaattgc ctgcatggcg
    23341 ccaatcgcca gtgggtcgtt gtacacaaaa accccatccg gccgtggccg ctgtttgagc
    23401 aaaaggcgca tggcctctgc tcccattttt tcgctgtcaa tgtctactgt ggaacgcgac
    23461 actaccaggg aatctgtaaa ggggagtcct ctgcgctgta gcgcctgctt atatccctca
    23521 aagcgacgcg ttccggtgct gttctcccgt ccgcggacat gcgcgatcct ccggcacccc
    23581 tggtcaatca gatgttcggt cgcaagccgt cccgcagcct cgtcgttgat accgacaaag
    23641 ttcgctgaaa gctgtggtat ctcacggtca atcagcacat acggcagttc gtggtggtcc
    23701 agacgcagaa aaggctgagg accggtgccg caggaagcaa tcaccagcgc atccaggcgc
    23761 cgtccggtaa gctgttgaat ttctctggcc tcaagctccg ggtcttcttc agaggatgaa
    23821 atgatggtcg aatatccgtg ctttgcaatc gccgccgaaa gcgccttggc cagctccgcg
    23881 aaaaatgggt gcagcaaatc agggacgatg aggccaatca gataactgcg cccggtaacg
    23941 agactccggg ccataatgtt cggttgataa tcaagctctt tgatgcgccg cagaacgcgt
    24001 tcccgggtct cttccgcgat gtcgggatgg tttcgcagga ccttggaaat ggtgacgacc
    24061 gagaggccca gatctttcgc gatgtccttc atgcggacag tcatatgtga ggactataca
    24121 atgttggaag tctgcgttga agttaaagat gaggtaaagt ttactttacc gatttccaaa
    24181 agaccggaaa ctatttttgc gagggtgaat gccaacacgc gcagttgttc gaccgggccc
    24241 ctctatgaaa tatcgcaggc tgggccagac agatttgcag gtttcggtac tgggctttgg
    24301 tgcttcgccg ctgggcgatg tctttggccg ggtggatgcc gctgaaggcg caagagcagt
    24361 ccatctggcc attgcggaag gcatccactt cttcgacgtc tctccttact atgggctgac
    24421 gctggcagaa gagcggctgg ggcaggcctt attgggcagg cgggacaagg tcgttctcgc
    24481 caccaagtgc ggaagatatg gcgctgctga gtttgatttc tctgccagac gggtccggtc
    24541 gagcatggaa gaatccttgc gccggctgcg caccgactat gtagacctgt ttcaggcgca
    24601 cgacgttgag tttggcgatg tggagcagat tgtccatgaa accattcctg cgatgcggcg
    24661 aatgcaggaa gaaggcaagg cccgctatat cggcatcagc ggatatccgc tgaaggtgct
    24721 gatgcgcatt gcagaaagtg ttcctgtgga taccatcctg agctactgcc actacaacct
    24781 tctgaacgac acgatggcat cggtcctggc gccctttgcg cagaaatcgg gcattggcct
    24841 tatcaatgca tccccgctgc atatgggagt gctgacggga aatggagccc cgcagtggca
    24901 tcctgctccg cctgaagtcc atcgcgcagt cgagaaagcg cgttcgttct gccgcgagca
    24961 caacatcagc ctgccggagc ttgcactgca atttagcctc gattgcacgc atgtggccag
    25021 cactctggtg ggcatgtcta cggaagaaga ggtgcggcaa aatctgcgcg cactggaaaa
    25081 accagcagat acggaattgc tgcggcaaat caaagcgatt ctggccccgg ttgcgaatgt
    25141 cgaatggccg tcaggaaggg cggaaaacca tgattaagcg cgtggatgct catcagcact
    25201 tctgggcgta ttcgcctgaa gagtatggct ggatagatgg aaatatggag gaaatccggc
    25261 gcgattttct gccgccgcat ctggccacgg aaatggccgg ggccggaatt tctacgagca
    25321 tcgctgttca ggcgcgccag acagttgcag aaacggaatg gctgctccat ctggcggagg
    25381 aacactcctg gattgcaggt gtggtgggat gggcgccgtt gatcagccct gattttcccg
    25441 cgctgctgga gcgcctgtgc gcgcatccga aattctgcgg cctgcggcat gttctgcagg
    25501 atgagccgga cgatgactac atgctcaggg acgggttcaa tcgcggaatc cgtctgctca
    25561 acggcaagga cctcgtctat gacattctga tctacgaacg tcatctgccc attgccctac
    25621 agtttgtgga ccgccaccca aaccagaaat ttgtgataga ccaccttgcc aagccccgca
    25681 tccgcgcagc agagatttca ccgtggcgcg aaaacattcg cgaaattgcg cgacggccaa
    25741 acgtctggtg caaactcagc ggaatagtga cggaagcgaa ctggcaaagc tggacgacgg
    25801 acacgcttcg gccttatgta gaaattgcac tggaagtttt cgggccacag cgcatcctgg
    25861 ccgggtctga ctggcctgtc tgtactctgg ctaccaatta ccagcgctgg tggcaaacgc
    25921 tggaggcgat gctttccagc ctttctgctt ctgagcagga cgctgttttt ggtggcaacg
    25981 ccatcgaaat ctacaacctg aaaggactga aggcatgaag gcgcttgtgc tgcgcggccc
    26041 gggatcggcc cagatagaag atgttcctcc tcccgtgcga agatccggcg aggcattgct
    26101 gcgcgtgcgc tatgttgggc tttgcgggac ggacctgaac tccttccgcg gacgcaaccc
    26161 gctcgtttcg tatccgcgga ttcctggaca tgagattgcc gcaaccatcg ctgagataga
    26221 cgccggtgct cccgggcttt ccgtggggat ggatgtgacg ctttctcctt acacaagctg
    26281 cggaaaatgc gcttcctgcc gaaacaacag aaacaacgcc tgccagttca accagacact
    26341 tggagtgcag cgcgacggtg ctttgacgga atacatctct gtgcctgtgg agaagctctg
    26401 cccggcacgc ctcagcctgc gcgaactttg cctggttgag ccgcttacgg ttggttgcca
    26461 cgctgtggcc cgcgcccgcg ttacagatgc ggacacggtc gccattttcg gctgcggagg
    26521 cgtcgggctg ggcgctgtgg ccgcatcggc cttccgtgga gcgaagaccg tcgccattga
    26581 cgtggatgac agcaagctgg acatcgcaaa gaaggccggt gctgcctttg ctgtcaacgg
    26641 acaaaagcag gacgcaaaga ctgcgctgaa cgaactgacg gacggcctcg gcccgcaggt
    26701 tgtgatcgaa gccgtgggca ggcccgaaac atttctctct gcggtggaca ttgtagcgtt
    26761 caccggacgc gtggtttaca taggctacgc caaagagcct gtgacctacg aaacccggct
    26821 ctttgtccag aaagagctgg acattctggg cacgcgcaac gcgcttccgc aggacttccg
    26881 cgaggtgatt gcaatgctcg agcagaagcg gttccctgta gatgcggcca tcagcaccat
    26941 agtgcctatg gaagaagcgc cgggggtttt cgccgcctgg gatgccgagc cttcgaggtt
    27001 cacgaagatc atggtttcct gcagtggtga cctcagataa cgataactgt atcgtagaca
    27061 aatttcaccc atgcttgcta tcatgggcac gcaccagcat tccataaaac ccatccttca
    27121 ggagccacag tgcagcgacg ggatttctgc aaacttctgg cggccacagc cgcatcgagc
    27181 gctctcccca ccttcggaca atctgctgct gagaaatctt ccgtgctgcc gcgcgaattc
    27241 aacgagtaca cgcaggacta cgccgagttc tgcgcgttgc cgccggaaaa gaggaccttc
    27301 tacgagctca gcaacggaag aatcgtcccg gtgaagctgg ataacgcaac gtggaaaccg
    27361 gcggaatggg gcaggccccc ggagctgcct gtcgccggcg gctcgtggga cggcgttccg
    27421 atggaatccc ccattcctga tttgtctggc gatggccctt acaagccgac atgggattcg
    27481 ctgctgcaat acgaagcccc ggagtggtat caggacgcga agtttggcat ctgggcgcac
    27541 tggagcccgc agtgcgtgcc cgaggccagc gactggtatg cgcgcaacat gtacatcgaa
    27601 gggcagcacc agtacaaata tcaccttgag cactacggtc ctccctcgcg cttcggctac
    27661 aaagacctct gcgcgcagtg gacgctgctg aactgggagc cggacgagct tatcacccgg
    27721 tacaaaaatg ccggcgcgag gatcttcatc gcactggcca atcatcatga cggcttcgat
    27781 acgtggaact ctaagcacca tccctggaat gcatctgcga ttggcccgca tcgtgatgtg
    27841 gttggcacat gggcgaccgc ggcgcgcaga cagggactgc gttttggcgt taccgtgcat
    27901 caggcgcgca actggtggtg gtttcagcct tcgcatggcg ccgacagttc cgggccgctc
    27961 gcaggtgttc cttacgacgg cattcttaca gaagcagaag gcaaggagca gtggtggcag
    28021 ggactcaacc cgcagctgct ctatggggtg aagcatcctt tggatgcgct gccggacatt
    28081 tcgtatgtca ggaacttcta tgaccgcacg cgcgatctga ttgatcagca caatcctgac
    28141 ctgctttact ttgacaattc gctgctgccg ctcggatggg gtggaatgaa catcggcgcg
    28201 tatttttaca acaacagcct gaagcagaat ggcggaaaaa tggaagccgt cctgaatgtg
    28261 aaggacgtgc ctgagcgcct ggtgaaagcc gtggtcgccg actttgagcg cgggctgacc
    28321 agcggcatca tgaaatatcc ctggcagtcg gagacctgca ttggcgcctg gcactatgcc
    28381 cgctttctat atgaaaagcc cggtgaatac ggtggttatc tgccgccacg cgatgtcatt
    28441 cactggatga ttgacacggt gagcaagaac gggaccttca ttctgaacat tcccggcagg
    28501 cccgacggca cgatagacag caaggaaatt gccgtgctcg acggcatcac cgcctggatg
    28561 caaaccaacg gcgaggccat ttacgaaacg cgcccgtgga aggtctatgg cgaagggccg
    28621 aaccagatca aggcaggatc tttccaggga aacagcatca gtaagctcgg ccccaaagac
    28681 atccgcttca cccgcaacaa ggcaaatact gttgtctacg caattgcgct gggctggccg
    28741 caggaaacca tcgtgattca gtcgctggga ctttctgccg aagccaaacc gggcaaagtg
    28801 gcaaaagtgg aactgctggg caccggcgcg aaggtcactt ggaagcagag tgcagatgct
    28861 ctccgcatag acctgcccaa acagtaccaa cccaaagtgg attacgcggc cgcgctgaag
    28921 gtgaccctgg cctgatttaa aaagaccctg atacctgggt gaccaggtac cagggtggag
    28981 gcagattgca ctgcaaaaag ctttagaaat acagctttcc tccgaactgg aaaactcgtg
    29041 ggtccgctgt gccgttgatt tgccctgcag tggagttata tgggctggaa gggttcccgg
    29101 taagcactcc tgtattcact ccggtgaatt gtgtgtggtt ccaggtgttg aagctctctg
    29161 cccggaactg gaacccggcc ctgtcattga agcggaaatc cttatacaaa gacaggttcc
    29221 agttgtcgcg ccccggcccc ttcacaatgt ttttaggtgc attgccccag gtgagtggcg
    29281 caggttgtgc aaaggcggtt ggactgaacc actgctggaa cttgtgcgga tacgtgatgt
    29341 gtccaatcag gtccgcatgg ttcgtagtcc cgccgccaaa gcccagattg tcgttcgccg
    29401 cattgacgct cagcgggttc ccggattgca tcagtgtaat accggagacc gtccacccgc
    29461 ccagcagggt cctcgtaatc ccgctcgaat gccggaagac aggcaggttg tagtcaaagc
    29521 tgactacggc gatgttccgg cggtcgaacc ctgctgttcc atactgatag cgcggattca
    29581 taggatcatc gagattattg aagagctggg catcaat
//
LOCUS       DASRBV010000039         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_255156, whole genome shotgun sequence.
ACCESSION   DASRBV010000039 DASRBV010000000
VERSION     DASRBV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255156"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /gene="ileS"
                     /locus_tag="VFA02_01775"
     CDS             complement(<1..2013)
                     /gene="ileS"
                     /locus_tag="VFA02_01775"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZU08604.1"
                     /translation="MSSKPEPGSAYPPLKETLTLPQTDFPMKANLPQNEPLRLKKWDE
                     EKLYQQVRETRKGSPRYLLHDGPPYANGPIHLGHALNKCLKDFIVKSKTMAGFDAPYI
                     PGFDCHGLPIEIKVDEQFGRKKLEMSSTEFLRACREYAQKYVDLQTSQFVRLGVFGRW
                     EHPYLTMSRQYEAKTLEIFYRFFEQGFVYKGLKPVYWCIHDRTALADAEIEYEMHTSP
                     SIYVKYALTSDPAAIGASLAGKNVSTIIWTTTPWTLPASLAVAFHPELDYVALEQDGR
                     VYIVAEALAASVRENCNLQDAKEVARFKGAKMERVTFQHPFLDRSILGVLADYVTTEQ
                     GTGAVHTAPAHGADDFYTGAKYGLDQTCNVDNAGRLRNGLPEYDGQTVFKANEPIIKL
                     LESRGALMGRSDIYHSYPHCWRCHKPVIFRATEQWFIAIDTPMGETTFRGLALEQIRK
                     NIKWDPAWGEDRIANMIETRPDWCISRQRIWGVPIAVFLCNKCHQPLKDAAINRTIVG
                     LFEREGVDAWHTREVEQLLPEGTKCPCGGSDFRKEMDILDVWFDSGVSWHAVLEAEQG
                     IAPPADLYFEGGDQYRGWFHTALLTSVGVSEKHQAPYTMVGTSGWTLDEQGRAMSKSL
                     GNVVDPVDIANRLGAEIVRLWVASVDFREDVSASENLMQRIADNYRK"
     gene            2298..>3406
                     /locus_tag="VFA02_01780"
     CDS             2298..>3406
                     /locus_tag="VFA02_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU08605.1"
                     /translation="MGNQVLTPPELEPGKSRPQDEQPELLVEADGSMWQSLLRNLRDA
                     FSSSKQPPLQLTSQPDANGELVETLEEEPIWKSLWTNLQDALFPKKLPPLELTSKPAE
                     TTAILNIKRNPKSSAYSIAAHVLIIGLIVLAIIEMRIHTKAIQKAQFAPVDISPYIPM
                     APAKDVMGGGGGGGNHELVDASKGKLPKFDKQQITPPQKLLVDKPKIPVPPTVVMPNI
                     KMPDANMPNLGVPQSTQVALASQGQGSGSGFGRGNGGGIGPGSGNGIGPGSGGGTGGG
                     IYHPGGGVSAPQVIYAPDPEFSDEARRAKYQGICVVALIVDAQGNPQNVHVVRPLGMG
                     LDEKAVEAVRQYKFKPAMFQGHPVPVEVNVEVNFR"
BASE COUNT          729 a          949 c          995 g          733 t
ORIGIN      
        1 cttgcgatag ttgtccgcaa tgcgctgcat caggttttcg ctggcggaga cgtcctcgcg
       61 gaaatccacc gacgccaccc agagccggac gatttcagcg ccgagacggt tggcgatgtc
      121 caccggatct actacgttgc caagcgactt ggacatggcg cggccctgtt catcgagcgt
      181 ccagcccgag gtgcctacca tcgtgtaagg cgcctggtgc ttttcgctga cgcctacaga
      241 agtcagcaga gcggtatgga accatccgcg atactggtcg ccgccctcaa agtaaagatc
      301 tgcgggcgga gcaatgcctt gttcggcttc cagcacggcg tgccagctta ctccggaatc
      361 gaaccagaca tcgagaatat ccatctcttt gcggaagtcg ctgccgccgc acggacattt
      421 tgtcccttcg ggcagaagct gttccacttc gcgcgtgtgc cacgcgtcca cgccttctct
      481 ctcgaacagc cccacaatcg tgcggttgat ggcggcatct ttcaagggct gatgacactt
      541 gttgcagaga aagacggcaa tgggcactcc ccagatgcgc tggcgcgaga tgcaccagtc
      601 cgggcgcgtc tcgatcatat tggcgatgcg gtcttcgccc catgccgggt cccacttgat
      661 gttttttctg atctgctcca gcgcaagccc gcggaaagtc gtttcgccca tcggtgtgtc
      721 aatcgcgatg aaccactgtt ccgtcgcgcg gaagatgacc ggcttatggc aacgccagca
      781 gtgcggataa gagtgataga tgtcgctgcg gcccatgagc gcgccgcgag attccagcaa
      841 cttgatgatc ggttcgttgg ccttgaagac cgtctggccg tcatattcgg gcagcccatt
      901 gcggagacgg cctgcattgt ctacattgca agtctggtcg aggccgtatt tcgcccctgt
      961 gtagaagtca tctgcgccat gtgccggcgc ggtgtggact gccccggttc cctgctccgt
     1021 cgtgacgtaa tcggcaagga caccgaggat actgcggtcc agaaacggat gctgaaaggt
     1081 cacgcgctcc atcttcgccc ctttgaagcg ggccacttct tttgcatcct gcagattgca
     1141 gttttcgcgg acagaagcgg ccagcgcctc ggcgacgatg taaacccgcc cgtcctgttc
     1201 gagcgccaca taatcgagtt cgggatggaa tgcgacagca agcgaggcag gcagcgtcca
     1261 gggcgtcgtc gtccagatga tggttgagac gtttttgcct gcgagagatg cgccaattgc
     1321 cgccggatcg ctggtcagcg cgtatttcac gtagatgctg gggctggtgt gcatctcgta
     1381 ctcaatctcg gcgtcagcca gcgctgtgcg gtcatggatg caccagtaga ccggcttgag
     1441 gcctttgtag acgaagccct gctcaaagaa gcggtagaaa atctcaagcg ttttggcctc
     1501 gtactggcgc gacatcgtga gataaggatg ttcccagcgg ccaaacacgc caagccggac
     1561 aaactggctc gtctgaaggt ctacgtattt ctgcgcgtat tcacggcagg cgcgcagaaa
     1621 ctcggtcgag gacatctcga gctttttgcg gccaaactgc tcgtctactt tgatctcaat
     1681 gggaaggcca tggcagtcaa agccaggaat atacggagca tcaaaccccg ccatggtttt
     1741 cgatttgacg ataaagtctt tcaggcactt attgagcgcg tggcccagat ggatagggcc
     1801 attggcatag ggcggaccat cgtgcagaag atagcgcgga gagcctttgc gcgtctcgcg
     1861 gacctgttgg tagagctttt cttcgtccca ttttttcagg cgcagaggct cattctgcgg
     1921 caggtttgcc ttcatgggaa aatcggtctg tggcagtgta agcgtctcct tgagcggagg
     1981 gtaggcactg ccgggttcgg gcttggagga catcacttct cccacggaaa atttgatagg
     2041 ggtaaaactt tattgtatct gtttaactgc gtgactttgt tgctgaccca tcgtctatat
     2101 tggaagaatc aggccggtga cagagcgtgg ccgcccgccc ccgtggatgc ttcgctcagg
     2161 atgacacgct aattttaggc agaacaggct tcgctgatga cgaaagaagg aatcatagtt
     2221 aaagacaagc gaggcacacg tcgtccataa tggagagata cggtcagatt aatcttttta
     2281 tctgaccgtg aagtgctatg ggcaatcagg ttttgacacc tccggaactg gagccaggaa
     2341 aaagccggcc gcaggatgaa cagcccgaac ttcttgttga agctgacggc tcgatgtggc
     2401 agtcgctgct gcggaacctg cgcgacgctt tttcctcatc gaaacaacca ccgctgcagt
     2461 tgacttctca gccagatgcg aacggcgaac ttgttgagac gctggaagaa gagccgatct
     2521 ggaaatcact ctggaccaac ctgcaggatg cgctctttcc aaagaaactt ccaccgcttg
     2581 aactgacttc aaagccggct gaaaccacag ccatcctcaa tatcaagcgg aacccgaaat
     2641 caagcgcata ctccattgct gcgcacgtgc tcatcatcgg cctcatcgtg ctggccatca
     2701 ttgaaatgcg catccatacc aaggccatac agaaggccca gttcgcccct gtggacatct
     2761 cgccgtacat tcccatggcc cccgcgaaag acgtcatggg aggcggtggc ggcggcggaa
     2821 atcacgagtt ggtggacgca tccaaaggca aattgcccaa gtttgataag cagcagatca
     2881 cccctccaca aaaactgctg gtggacaaac caaaaatccc ggtgcctcca acggtagtga
     2941 tgcccaacat caagatgccg gacgccaata tgcccaacct gggcgttccg cagtctacgc
     3001 aggtggccct ggcttcgcag gggcaaggct ctggctctgg attcggccgt ggaaacggtg
     3061 gcggtattgg cccgggaagc ggaaacggca ttggcccggg ttctggcggc ggcaccggcg
     3121 ggggcatcta tcacccgggc ggtggcgttt ctgccccgca ggtgatttat gcgcctgatc
     3181 cggagttttc cgacgaagca cggcgtgcaa aatatcaggg aatctgcgtg gtcgccctca
     3241 ttgtggacgc gcagggaaac ccgcagaacg tgcatgtagt gcggccgctg ggtatgggcc
     3301 tggatgagaa ggcagtcgag gccgtgcggc agtacaagtt caagcctgcc atgttccagg
     3361 gacatcctgt tccggtggaa gtaaatgtag aagtaaactt ccgtat
//
LOCUS       DASRBV010000040         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_205695, whole genome shotgun sequence.
ACCESSION   DASRBV010000040 DASRBV010000000
VERSION     DASRBV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205695"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFA02_01785"
     CDS             complement(<1..801)
                     /locus_tag="VFA02_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU08606.1"
                     /translation="MSHLYAAVVGTIILTLLIVSLTRLGKVKTRADYLVAGRSLPAFV
                     LIFTLLSSWIGSGSLLGGAENAYNNGIVALWQGAGGWAGLLLIYFIAPRARRSAQFTI
                     PDLLEARYNQLARVLGVIAVLFAYTAITSYQLIGGGDILSLIFPTVITPDLGKYIIAI
                     FVIVFTAIAGMSSVAYMDVVIGLLATITMLVAFPVLLHMSGGWSGFHAALPASHFRPL
                     GSGPFIRAMELFLPTCLLMLGNQAMYQKFFSAKSEKDARVAVVGWIIGT"
     gene            complement(891..1199)
                     /locus_tag="VFA02_01790"
     CDS             complement(891..1199)
                     /locus_tag="VFA02_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZU08607.1"
                     /translation="MISLKESSSGVSFAVRVQPRASRTAISGVLGEGDNAALKISLTT
                     PPVEGQANEALVRFFAELLNVPRSSVEIISGSQSRNKVVRIREMSAEQVRSRLRQSSG
                     "
     gene            complement(1196..1948)
                     /locus_tag="VFA02_01795"
     CDS             complement(1196..1948)
                     /locus_tag="VFA02_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZU08608.1"
                     /translation="MPDFLSIQERLSSVLSLIHAACRRAGRNPDEVHLMAVSKTHPAE
                     AIAAAFQAGIRLFGENRVQEFQQKSSGLKVFGIGVKEMDAGKQQLAEVHLIGHLQSNK
                     AAKAAEIFSAIDTVDSLRIAQRLNEAAARLNWTIPILLEIKLSAEETKTGLAPGSPEL
                     YQLLEHLPDLKNLALRGLMTVAPLDENPVTARTCFRTLRKLRDQLATQHPQLDFGELS
                     MGMSGDFEIAIEEGSTLVRIGTAIFGTRSKPA"
     gene            2192..>4038
                     /locus_tag="VFA02_01800"
     CDS             2192..>4038
                     /locus_tag="VFA02_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08609.1"
                     /translation="MRLAYALLVSLLLPGLLFAQGIVTGYITGTVVDPQGAVVPNASV
                     TAVQASTNARFVVNTLSNGDFELRNLPPGRYTVTISAPGFQSTTLSNVVVAVGVETAL
                     KNQTLSVGQSQQVVTVDATMTPLLSTTQSQISTEFTPQQIVALPTNAGFDTLALTAQP
                     GVASAHDAMFANSNGSFGYSGLTVNGERGRSVNFEIDGQNNNDNSVTGPQFFFNNGEA
                     LQAVQVVTGVYGAQYGRSTGAVVNYVTKSGTNEFHGSAFEFYTGSFLRSLTNDQKNPL
                     LGFCAPGQSPSTGCAVPTVPRYVEHQYGGTLGGPILKDKLWFFGSTFWDPIHEGAAPA
                     NSAPNLTPTPNGLQQLQAAFPNNPSVNALTQFGPFAIKYGNPVVAGATVMKPVTVGNT
                     TQMVEFGPVQRFAPATQRMQQHLGRVDWQPNTTDHLFLRYMYYDALQAGALSGTSSAD
                     IAQGDFYNVPSTNHSIGADWTRTFTSNWVNQLRYSFQQAKIVFTPGALTTCTINNLTA
                     CPASVVFSGNDESFGLASNMPQGRTVKVNQVQDNATWIHGNHSFNFGGEFQKQNSPNA
                     GLANYNGAYSFASFNSFMQGGSNANDLISLADGSPVLPFREPDVDGYIQ"
BASE COUNT          935 a         1048 c         1130 g          925 t
ORIGIN      
        1 tgtgccgatg atccacccca caaccgctac gcgcgcatct ttttccgatt ttgccgagaa
       61 gaacttctgg tacatggcct gattgcccag catcagcagg catgtgggca gaaacagctc
      121 catggcgcga atgaacggcc cggatccaag cggacggaaa tgcgatgcgg gcagcgccgc
      181 gtgaaagccg ctccagccac cagacatgtg cagcaaaaca ggaaaggcaa ccagcatggt
      241 gatggtggca agcagaccga tgacgacatc catgtatgca acggaagaca ttccggcaat
      301 ggcagtgaac acaatcacga agatggcgat gatgtatttg cccagatccg gagttatcac
      361 ggttggaaag atgaggctga ggatgtctcc gccgccgatg agttgatagc tggtgatggc
      421 ggtgtaggcg aagagcacgg caattacgcc aaggacgcgc gcgagctggt tgtagcgggc
      481 ttcgagcagg tctggaatag tgaactgggc cgagcgccgt gcgcgcggag caatgaaata
      541 aatgagcagc agtcctgccc agccgccggc gccttgccac agcgccacga tgccgttgtt
      601 gtaggcattt tccgcgcctc cgagcagcga tccggagcca atccaggaag aaagcagtgt
      661 gaagatcaaa acaaatgcag gaagcgagcg ccctgcgacc aaataatcgg cccgtgtctt
      721 tacttttccc agacgggtaa gcgaaacaat aagcagagta aggataatgg tgcccaccac
      781 tgcggcgtac aagtgactca tggtcttcct ttgttgaagg cgtaagcgac agtgtacggg
      841 gaaatgtaag gaagaggaaa tccaggtgcg cagaggcatt ttcggagagg ttagccgctg
      901 gattgtctca gtctggagcg tacctgttcc gcgctcattt cccggatgcg cactacttta
      961 tttctggact ggctgccgct tatgatttcg acggatgaac gcggaacatt cagcagttcg
     1021 gcgaagaagc ggaccagggc ctcattggcc tgcccttcaa ccggcggagt agtcagggaa
     1081 atcttcaatg cagcattgtc gccttcgccc agcacacctg agattgctgt ccggctggcg
     1141 cgcggctgca cacgaacagc aaagctcacg cctgatgaag attctttcag ggaaatcatg
     1201 cgggtttcga tcttgtgcca aagatggccg tgccaatgcg caccagcgtg gagccttcct
     1261 caatcgcaat ctcgaaatcg ccggacatgc ccatggaaag ctcaccgaaa tcaagctgcg
     1321 gatgttgggt ggccaactgg tcgcgcagtt tgcggagcgt ccggaagcag gtccgcgctg
     1381 ttacgggatt ctcgtcgagc ggagcgacgg tcatgaggcc acgtagggcg aggtttttca
     1441 ggtcgggcag atgctccagc aattggtaca gctcagggga gcccggggcg aggccggtct
     1501 tggtttcttc ggcgctgagc ttgatttcaa gcaggatggg gatggtccag ttgaggcgtg
     1561 cggcggcttc gttgaggcgc tgagcgatgc ggagtgagtc tactgtgtct attgcggaga
     1621 agatttccgc tgcctttgcg gctttgttcg attgtagatg cccgattagg tggacttcag
     1681 ccaattgctg cttgcctgcg tccatttctt tcacgccgat tccgaacact ttcagacctg
     1741 aggatttctg ctgaaattcc tgcaccctgt tttcaccaaa gagccggata ccggcctgaa
     1801 aggcggcggc gatggcctct gccggatggg ttttagagac cgccatcaga tgtacttcgt
     1861 ccgggttacg cccggcgcgg cggcaagcgg catgaatcag gctgagaacc gaggagaggc
     1921 gttcctggat ggaaaggaaa tcgggcattt tgtttagttt acggtagatg ttctacagaa
     1981 aaatgtatat ctctcacaag aaatcgtcga accaaagtga ttttttataa ggagaaggtg
     2041 ttttggcccg catactccta ttcttttctt ttaattaata agtttgacaa cttcttgacg
     2101 gaaaacggtt attaaattgc caccactgtt catgcaagta aaggatacaa tttccgagaa
     2161 gggaagaaaa atacatatgt ctcgatggac gatgagattg gcatatgcgc tgcttgtaag
     2221 cctgctattg cctggtttgc tgtttgcgca gggtattgtt accggctaca tcactgggac
     2281 agttgttgac ccgcaagggg ccgtggttcc aaatgcctct gtgactgcgg ttcaagcatc
     2341 aacgaatgcg agatttgtag tgaacacgtt gtctaatggt gattttgagc tgcgcaatct
     2401 cccgcccggc cgttatacag tcaccattag cgctccaggg tttcagtcca cgacgctttc
     2461 caatgttgtc gtcgcggtgg gtgtggaaac tgcgttgaaa aaccagacgc tctctgttgg
     2521 ccaaagccag caggtggtca cagtagatgc aactatgaca ccgctgcttt ctaccacgca
     2581 gtcccagatt tcgacagagt ttactccgca acagattgtt gccctgccta ccaatgccgg
     2641 gttcgacact ctggcgctga cggcgcagcc gggcgttgcc agtgcccatg acgcgatgtt
     2701 tgcaaacagc aatggaagct ttgggtattc gggacttact gtgaatggcg agcgcggccg
     2761 ctctgtcaat tttgaaattg acggccagaa caacaatgac aactcggtaa caggtccgca
     2821 gtttttcttc aataacggcg aagcacttca ggccgtccaa gtggtgaccg gtgtttacgg
     2881 tgcacagtat ggccgtagta ctggcgccgt agtcaattac gtcacaaaat ccggaaccaa
     2941 tgaatttcat ggttcggcat tcgagtttta tactggctct tttctgcgct cgcttaccaa
     3001 tgaccagaaa aacccactgc ttgggttctg cgctcccggg caatctcctt ctacaggctg
     3061 tgctgtgcca accgtgccgc gctatgtaga gcatcagtat ggcggtactt tgggtggtcc
     3121 tattcttaag gacaagctct ggttctttgg cagcaccttc tgggatccga tccatgaagg
     3181 cgcggccccg gccaactcgg cccctaatct tactccaact ccgaatggcc tgcagcagct
     3241 ccaggcggcc tttcccaata atccttctgt aaatgctctg acccagtttg gcccgttcgc
     3301 tatcaaatac ggcaatccag ttgttgctgg tgcaacggtc atgaaacccg taaccgtagg
     3361 aaacactacg cagatggtcg aatttggccc agtgcagcgc tttgctccag caacgcagcg
     3421 aatgcagcaa cacctgggca gggtggactg gcagccaaac acgacggacc atctttttct
     3481 gcgctacatg tattacgacg ccctgcaggc cggcgccttg tctggcacga gtagtgccga
     3541 tatcgcgcag ggagactttt acaatgtgcc gagcacaaac cactccatcg gcgcagactg
     3601 gacacgtact tttacctcaa actgggtaaa ccagctgcga tatagcttcc agcaggcaaa
     3661 gattgtattt actcctggag cattgactac ctgcaccatc aataatctga cggcttgccc
     3721 ggccagtgtg gttttcagcg gtaatgatga gagctttgga cttgcctcaa acatgcccca
     3781 ggggcgcacg gtcaaagtaa accaggtgca ggacaatgca acctggatcc acggcaatca
     3841 tagcttcaac ttcggcggcg aattccagaa gcagaattct cccaatgctg gtcttgccaa
     3901 ctacaatggc gcttactcgt ttgccagctt taactccttc atgcagggag gatcaaatgc
     3961 aaacgacctg atcagtcttg cagatggcag cccggtgttg ccattccgcg agccggacgt
     4021 agacggctac attcagga
//
LOCUS       DASRBV010000041        13747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_2068,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000041 DASRBV010000000
VERSION     DASRBV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..736)
                     /locus_tag="VFA02_01805"
     CDS             complement(68..736)
                     /locus_tag="VFA02_01805"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HZU08610.1"
                     /translation="MVRDGYYYGFALIAVAIVVYLLTDGWGWAILPLLLAAFFLWFFR
                     DPERAIPQGDGLVVSPADGKLTSIERITTPKGERLRLSIFLSVFDVHVNRSPVSGVLR
                     EVQYQKGLFLNALNPACSEKNEQNLAVLETPEGHEVAFKQIAGLLARRIVFRPQVGDY
                     LERGQRVGMIKFGSRVDVLLPGHAELRVKQGDRVKGGATILAQIPELILGPMLAELEV
                     ETLV"
     gene            complement(838..1047)
                     /locus_tag="VFA02_01810"
     CDS             complement(838..1047)
                     /locus_tag="VFA02_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HZU08611.1"
                     /translation="METFISPDSEAIRRLEEQHHHYSEKLESLLQKPYLSAEEQLEEV
                     RLKKLKLSVKDQIEALRNGMYHHVA"
     gene            complement(1115..1561)
                     /gene="rimI"
                     /locus_tag="VFA02_01815"
     CDS             complement(1115..1561)
                     /gene="rimI"
                     /locus_tag="VFA02_01815"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZU08612.1"
                     /translation="MTYFVRRMEGRDIDAVLKLERNVPEAPHWKRAAYEGRTGFVAES
                     GGQLIGFAMGRLVADVCELESIVVAVEARGQGVGKALFRAVVEWAQSCNALRLELEVR
                     ASNIRAVSVYRSAGMQSEGVRPKYYASPEEDAILMGMSLGGGGKPA"
     gene            complement(1558..2190)
                     /gene="tsaB"
                     /locus_tag="VFA02_01820"
     CDS             complement(1558..2190)
                     /gene="tsaB"
                     /locus_tag="VFA02_01820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZU08613.1"
                     /translation="MILGMDTCGPVGTLALAQLDGDRICLRGEAEIAGKTYSAMLMPR
                     IRELLDANGIGVHEITAIVVVNGPGSFTGVRVGLSAVKGLVEALGIPVIAVSRLAVLA
                     QKGGVKFAALDAGRGEFYFGDYADGQHEALLSVEEVRSACPEREIAICEDRVALALPE
                     ARVVGAPTATDALRFGLPKLLTQDFDDAVMLDGNYLRRADAEVKMGALKR"
     gene            complement(2296..2703)
                     /gene="mce"
                     /locus_tag="VFA02_01825"
     CDS             complement(2296..2703)
                     /gene="mce"
                     /locus_tag="VFA02_01825"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZU08614.1"
                     /translation="MTKIDHLGIAVKSIAAARGLYESLGLRVMQEETVEHEKVRVAMV
                     PVEDSRIELLEPVSDDSPVGKFLAKRGEGLHHVALHVDDISATLEGLKNKGVRLVSDE
                     LQIGAGGHLYFFVHPSSTGGVLLEICQDPIADV"
     gene            complement(2690..3646)
                     /gene="meaB"
                     /locus_tag="VFA02_01830"
     CDS             complement(2690..3646)
                     /gene="meaB"
                     /locus_tag="VFA02_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZU08615.1"
                     /translation="MSNPEIAQLLGRVRAGDVRALARAISLVENDSPLSQEVLSACFS
                     YAGRALRIGITGAPGAGKSTLVDRLARHYRGLGTTVGVIAVDPTSPFTGGAILGDRIR
                     MQEGLHDSGLYVRSMATRGSLGGLARTTADVVSVIEASEKKIILIETVGVGQDEIDIV
                     RLADITLVVLVPGMGDDVQSIKAGIMEIADIFVINKADREGADRVEREVRAMQSLGTK
                     HEDHVPAVIRTVATTGEGLDKLASAIEEMKEWLQAEGRLEARRRRYWRERLVQMVLDS
                     LMRQLREHGLTDAELDEHAGRIARGEEDPYLLVPRLVAYDKD"
     gene            complement(3872..5065)
                     /locus_tag="VFA02_01835"
     CDS             complement(3872..5065)
                     /locus_tag="VFA02_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU08616.1"
                     /translation="MPVEADLQSPATYPFGLTEEQQQLRKEIREFAQREIAPNVMRWD
                     EASEFPVGVVKQLGQMGLMGIIFPVEYGGSGLGYVEYVTAIEELSAVDGSIGIIVAAH
                     NSLCTNHIFLAGTEEQRRKYIPKLASGEWLGAWGLTEPNSGSDAAGAKTTAVRKGDKW
                     VLNGNKTFITNGHYADVAVVIAVTDRSQGTHGLSAFIVEKGTPGFRPGKKENKLGLRA
                     SDTSELIFEDCEIPAENLLGKLGEGFVDSMRVLDGGRISIAALSLGIARGALDASLRY
                     VKERRQFGKAIAEFQGVQWKLADMATELDAAKLLTLRAAVLKDAGQRVTRESSMAKLY
                     ASEVAVRICDQAVQLHGGYGFIKDYPAEKYYRDVKLCTIGEGTSEIQRMVIAREILKV
                     VPSRG"
     gene            complement(5167..5877)
                     /locus_tag="VFA02_01840"
     CDS             complement(5167..5877)
                     /locus_tag="VFA02_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08617.1"
                     /translation="MKKEALHVSSPPAPGPFDAALVETGRFPDHEETPEFVRNELRPW
                     LRKLAHEHSLQPAAWIQGADLHEGNAADLVRKHGAQWIPETGLGVWPDREPPRLWSAE
                     DFHELDPGQKIRPMMYTVFHVSGGTEASEHALSTLAGSGTLIYALLASAPEEFHRNAT
                     ERFLPEIREEALRGYPFYMPLLDIHSLQTGLSNPDTFHSWMGTARVYVRESAEDRGIL
                     LISGIRNALRDFLESIRL"
     gene            complement(5874..6770)
                     /locus_tag="VFA02_01845"
     CDS             complement(5874..6770)
                     /locus_tag="VFA02_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08618.1"
                     /translation="MNSMEVAQTDRRRRLEPVERDIQLKPFLRAHELEDVSLYWAPVD
                     SFPMSFRVRTWWMNFLTRFRKLMAERDLRQENFLQFKTVTAEKSGHFMSNALDFVPVA
                     GLGLKPGATLPPLPEADQLRKDSLQADFSMDKHIPDYTYWFVRKQDEEQRQHFFGMGG
                     MVFLWLKEDPITRPPDLKLPPSVRRMLESRGSNIQQHLQTTYALLDSFQKKSKIVFGK
                     NIEGDPAFMNASFVLPLLSSRNFFDAGPEIRQQWFEVFDGYCIESRDDKGILLALKDP
                     AFDAPLSDLLEQMHGEGMVYPL"
     gene            complement(6808..7287)
                     /locus_tag="VFA02_01850"
     CDS             complement(6808..7287)
                     /locus_tag="VFA02_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08619.1"
                     /translation="MLSLLASGFQVEHFGNGRLMIVNPPVMWVSVTCYVFAVIGLLLL
                     LSGINARSRSTGVMGALLLVCFALLGWTTSVPSTAVLDKDAGLLRIHIGWAHVTSTYP
                     LQQVKYATVESTTAAYRLVFVLDDGHRVGLGSYSDQQGQPEAAHAINQFLGANDIQP"
     gene            complement(7307..8038)
                     /locus_tag="VFA02_01855"
     CDS             complement(7307..8038)
                     /locus_tag="VFA02_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08620.1"
                     /translation="MSAFNGTVLTPLAVADPGTDHKLPAAGSLNYGAITSQTALAGTN
                     GVDTCLVKGNEWQHFAGNYTEFIDANYQLTVKGNRQETLQSDQKLTVVGKTNETFIGV
                     HNRTNIAPSNQTYVHTLTQQHSQPSHVNQPTTYQRHENNVLEYFQEHFEHAWYKIGVV
                     GLNIDTTALLKLQWASIYLQDSFVRNKNTAAINLEAEPLSVKIKGLMSKIDWVQVGLA
                     MAYAHGVPLDVNAGIDVNELVIPPF"
     gene            complement(8125..8766)
                     /locus_tag="VFA02_01860"
     CDS             complement(8125..8766)
                     /locus_tag="VFA02_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08621.1"
                     /translation="MSVGTAHCAEVIAHAQVVVSGGKKASNTGVVVWLQPPYLVSAAK
                     PSHFRLVQKDKKFTPHLLVIPLGSTVDFPNLDPFFHNVFSQFNGKRFDLGLYEAGSTK
                     AVRFDHEGVSYIFCNIHPEMSAVVIALATPYVAESAADGSVVIHNVPEGDYELHVWAE
                     GADTQQLQAFTRAVHVRSPRTDLGDIHITRSSDVLAHKNKFGEDYKPSENTLY"
     gene            complement(8820..10274)
                     /locus_tag="VFA02_01865"
     CDS             complement(8820..10274)
                     /locus_tag="VFA02_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08622.1"
                     /translation="MFTGLFLAGAAIAQQPVSGDAATVQLEKRLDQVLVQLDGMRHQL
                     DDSQKEIQQLRVELQHMRMQLAENNAGESAQIVSDLRSSVQQLQEQSDIVQSEVKQHD
                     QTKVESMSKYPLQVSGMVLMTSLLNDGAVDDADLPIIAVAKMPDNAHGSLTATARQSI
                     LGLNAEGPTLWGAHSAADLRVDFFGGIPYADYNTSAGILRLRTAHARLDWGHDSLAVM
                     LDAPVISPLNPTSYLSVGEPALAWSGNLWTWSPQLAWKHTLPVHGSKAGFEFALIDPA
                     SPGPPQSYAVRGPGAGERSRQPGYESRMSWNTHAGDQTIEIGAGGYYSRQSYPYGHHA
                     DAWAVTADWSVPFTSHLELSGELYRGRAIGGLGGSVYKDYVNYAEYPHFQGLDTEGGW
                     AQLKFRLLRNLEANFAAGQDGGFAQELRESQFASNQDNYGNLARNRTLLANFIFRPKT
                     YLLFSAEYRNIHSWPIVGSLNASQSVGLAAGYLF"
     gene            complement(10325..12112)
                     /locus_tag="VFA02_01870"
     CDS             complement(10325..12112)
                     /locus_tag="VFA02_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU08623.1"
                     /translation="MRMRTLLLLCLFSVSFGLTALSLLIVHTILEKQIRRDIVSDLHR
                     SIMTFRNMQVQRRNMLQREAALLADLPSLKSLMTAPDTLTIQDAGAEFFRVSGSDFFA
                     LADRNSHLIALYETNGPSRGIPRIASAEANRVFLPEEPNYVLNNGRLYEVSSQPLYLG
                     PPQTGTLLGYVAVGYLIDSRVAREVSEASAAEVIFKANNNIVATTLDQPRWQEFTKYG
                     DGAFHAQETATDLWLRREHYVGATVPLAQNNDNHVQLVVLKSYDEAAQYLRRLDQLLI
                     VLGLCVLFAGAVIAVYISSTITRPLDSLVAGARALGAGDFHFQFQNRGARELQELGVA
                     FDRMRMQLEQARKELIEAERLATIGRMASSISHDLRHYLSAVYANAEFLGYESTNAAE
                     RAELLAEVRLGVEGMLELIDSLLIFSRTGQSLQPGYESLALIAERAITLVRAHRDTQG
                     IAITWDALPQIEVWLDVRKIERAIYNLLLNACQAAKNGADIPAVHLAIAETPEWINIS
                     IQDNGPGVPESIRVTLFQPFVSEGKQAGIGLGLTIANRIAEEHGGSVTLVESQPGRTI
                     FNLSLSKAMLNKIAESAQEKHPATLLPHN"
     gene            complement(12136..12825)
                     /locus_tag="VFA02_01875"
     CDS             complement(12136..12825)
                     /locus_tag="VFA02_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU08624.1"
                     /translation="MNTILVIEDDTRIQKALHRLFASEGYEVKGVSDGSHGLEAFSSG
                     TPDAVILDLMLPGVSGRELCRAMKQSSPDTPVIVLSAIAEVADKVLLLEIGADDYVTK
                     PFSPRELLARVQAAMRRNKRTRTSPSMHFGEVQVDFARMQVTRSGKRVTLTAHEFKLL
                     RFFLDNPERVLTREELLNDVWGYNFYPTTRTVDNQILKLRQKLETDPANPVHFCTVHG
                     VGYKFVQSPEK"
     gene            13030..>13747
                     /locus_tag="VFA02_01880"
     CDS             13030..>13747
                     /locus_tag="VFA02_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU08625.1"
                     /translation="MPIPNFFIVGAPKAGTDALFYDLEQHPEIYMSPLKEPCFFSSEI
                     RPENFHPSLQPQIEASRDETRRYLDAGMPGKRFGGIISTWEDYLKLFSCVTHEKAIGE
                     GSVCYLWSETAAAAIASAVPHARIVIVLMDPAERAFHQYLKSVSDGTVRHSFRKHLQM
                     AMQSGPELGVYHPFLAFGNYSDQVKRYMDQFPTHQLYISLYEELRGDYAGWLSRLLLF
                     LGVNPAFVPAHSEVPSTPHLL"
BASE COUNT         3245 a         3954 c         3328 g         3220 t
ORIGIN      
        1 atatttcctg ccgtaaacaa cgaaggcagg atatacattc cccggcgccg gcgcttgcct
       61 ctagccatta aaccagcgtc tccacttcaa gttcagccag catcggcccc aaaataagct
      121 caggaatctg cgccagaatg gtcgctccgc ccttcacccg gtcgccttgt ttcacgcgaa
      181 gctccgcatg accgggcagc agaacatcta cacgcgagcc aaacttgatc atgcccacac
      241 gctgcccccg ctccagataa tcgcccacct ggggacgaaa gacgatacgc cgcgccagaa
      301 ggcccgcaat ctgtttaaag gccacctcgt gtccttccgg agtttccaga actgcaaggt
      361 tctgttcgtt tttctccgag caggcagggt tgagcgcatt caggaacagg cccttctgat
      421 actggacttc ccgcaaaaca ccgctcaccg gggagcggtt cacatgcaca tcaaatacgc
      481 tgagaaagat gctgaggcgc aggcgctccc ctttgggagt cgtgatccgt tcaatcgagg
      541 taagcttgcc gtcagccgga gagacaacca gcccgtcacc ttgtggtatg gcgcgttctg
      601 gatcgcggaa gaaccacagg aaaaatgcag ccagcagcag gggcagaatg gcccagcccc
      661 atccgtcagt caataggtaa acaacgattg cgactgcaat cagggcaaag ccgtagtaat
      721 atccgtcacg aaccatgaga gattaattct ctcattatag agtggtggaa accttttcct
      781 gagggcacag gaaccccttt gggcccctat gccctgatat gtcagacccg tgccggatca
      841 cgccacgtga tggtacatcc cgttgcgcag ggcttcaatc tggtctttga ctgacaattt
      901 aagcttcttt aaccggactt cttcgagttg ttcctcggca gagaggtagg gtttctggag
      961 caggctttcg agtttctccg aatagtgatg gtgctgctct tccaggcggc gaatggcttc
     1021 actatccggc gagatgaatg tttccatcag gacacctcct ttttctctaa gacgtaccaa
     1081 gctaacaccg cgtttgccca ctggcaatgg tttttcaggc aggttttcca ccgccgccca
     1141 gagacatccc catcagaatc gcatcttctt ctggggatgc ataatatttt gggcgcacac
     1201 cttcgctctg cattccggca ctcctgtaaa cactgacagc tctgatattt gaagcgcgaa
     1261 cttcaagctc aagtcgaaga gcattgcagg actgcgccca ttccactact gcccgaaaca
     1321 atgcctttcc cacaccctgc ccgcgcgctt ccaccgctac cacaatggac tccagctcac
     1381 aaacatcggc aaccagtctg cccatggcaa agccaatcag ctgcccgccc gattccgcca
     1441 caaaccccgt ccggccctcg taagccgcgc gcttccagtg cggtgcctcg ggaacattgc
     1501 gctccaactt gagcactgcg tctatgtcgc ggccctccat cctgcgcaca aagtatgtca
     1561 tctcttcaga gcacccatct tcacttcagc atccgcgcgc ctcagataat tcccatccag
     1621 catcaccgca tcatcaaagt cctgtgtcag cagcttcggc aacccaaacc tcagcgcatc
     1681 cgttgctgtg ggcgcaccga ccacacgcgc ctctggcaac gccagggcaa ccctgtcttc
     1741 gcaaatggcg atttcccgtt caggacacgc actacggacc tcctcaacgg aaagcaatgc
     1801 ctcgtgttgc ccatctgcgt aatcgccgaa gtagaactca ccacgcccgg catcgagcgc
     1861 agcaaattta acaccgccct tttgggcgag cacagcaaga cgcgaaaccg caatcaccgg
     1921 aatcccaagc gcctccacca gccctttcac cgcgctcagt cccacgcgga ccccggtaaa
     1981 acttcccggc ccgttcacaa caacgatggc agttatctca tgcacgccaa tcccattcgc
     2041 atccagcaat tcgcggatgc gcggcatcag catggccgaa taagttttcc cggcaatttc
     2101 tgcctcgcca cgaaggcaaa tgcgatcgcc atccaattgt gcaagcgcga gcgtcccaac
     2161 agggccgcag gtgtccattc ccagaatcat aagtttttaa tcgctgctga gatgccccac
     2221 atcttgaaga aatctggatg cagaattcca cgactacaaa acgcaaccaa cctgtcttgc
     2281 catgcttaca aaaaatcaaa catcggcgat cgggtcctga cagatttcca gcaacacgcc
     2341 acccgtgctc gatggatgca caaagaaata aagatgccct ccggcaccaa tctgcaactc
     2401 gtcagagacc agcctcacac ctttattttt caggccttcc agcgtggcgg agatgtcgtc
     2461 tacatgcagt gccacgtgat gcagtccctc gccccgtttt gccagaaatt ttcctacggg
     2521 tgaatcgtct gaaacaggtt ccagcagctc gatgcggctg tcctccaccg gcaccatggc
     2581 cacacgcact ttttcatgct ccacagtctc ttcctgcatc acacgcaggc ccagggactc
     2641 ataaagaccg cgcgcagcgg caatactttt tactgcgatt ccgagatgat caatctttgt
     2701 cataagctac aagccgcgga accagaagat atgggtcttc ttctccgcgc gcaatacgtc
     2761 cggcatgttc atccaattcg gcgtccgtca gtccgtgctc gcgcaactgg cgcatcaatg
     2821 aatccaacac catctgcacc agccgctcgc gccagtatcg ccggcggcgt gcttccagac
     2881 gcccctctgc ctgtagccat tctttcatct cttcgatggc cgaggccagc ttgtcaagac
     2941 cttcgcctgt ggttgcaaca gtcctgatga ccgcaggtac atggtcctca tgtttggtgc
     3001 ccagcgactg catggcgcgg acctctcttt ctacgcggtc agcgccttcg cggtcggctt
     3061 tgttgatgac gaagatgtca gcaatctcca tgatgccggc cttgatgctc tggacgtcat
     3121 cccccattcc gggcactagg accacgagag tgatatcagc tagacgaacg atatctattt
     3181 cgtcctgccc cacgccgacc gtctcgatca gaatgatttt cttctcgctg gcctcgatga
     3241 cagagacaac gtcggccgtt gtccgcgcca atccgccgag tgagccgcgc gttgccatgc
     3301 tgcgtacata caggccggaa tcatgcaggc cctcctgcat ccgaatacgg tcgccaagaa
     3361 tcgccccccc tgtaaatggg ctggttgggt caaccgcgat gacgccaacc gttgtcccca
     3421 gcccgcgata gtgccgcgca agacggtcca ccagcgtgct cttgcccgct ccgggagcgc
     3481 cagtgatgcc gatgcgcaga gcgcgtccgg cgtatgaaaa gcacgcggaa aggacctcct
     3541 gggacaaggg agaatcattt tccaccagtg aaatggcccg cgcgagagca cgcacatcgc
     3601 ctgcgcggac gcgcccaaga agttgcgcaa tttcaggatt gctcatgagt ggcggcgatg
     3661 aaattgcctg gcttcatttc tgaaaagggg cctttttggc aggccccctc ttcatagtgg
     3721 gctctttcag agcattgcga atagcttcgt tgaccacctg ttttaatgtc attccgcgct
     3781 cacgcattgc acttctcaac attgcgttga catcattatc cagtgtgatc gtctttctca
     3841 tggttgcatc atagcattac aaaatagtgt ttcaccctct cgatggcacc accttcagta
     3901 tctcgcgtgc gataaccata cgctggattt cgctggtgcc ttcgccgatc gtgcacagct
     3961 tcacatcgcg gtagtatttt tccgccgggt agtccttgat aaagccgtat ccgccatgca
     4021 actgcacggc ctggtcacag atgcgcacag ccacttcgct tgcatagagc tttgccatgg
     4081 aagattcgcg cgttacccgc tgcccggcat ctttcaggac ggcagcgcgc aacgtcagca
     4141 gctttgcagc atccagctcc gtggccatat cggccagctt ccactgcaca ccctgaaatt
     4201 cagcaatggc cttgccgaac tgccgccgct cttttacata gcgaagactg gcatccagcg
     4261 cgcccctcgc aatcccgagc gaaagcgcag caatcgagat gcgtccgcca tccagcacac
     4321 gcatggaatc aacaaaccct tcgcccagct tgcccagaag gttttctgcc gggatttcgc
     4381 agtcctcaaa aatcagctca gaagtatcac tcgcgcgcag acccagcttg ttttctttct
     4441 tgcccggacg gaacccgggc gtaccctttt ccacgatgaa agcagacaag ccatgcgtac
     4501 cctggctccg gtcggtcacc gcaatcacca ccgccacatc agcataatgc ccattcgtga
     4561 tgaaggtctt gttcccatta agcacccact tgtcgccttt gcggacggca gttgtttttg
     4621 ctccggctgc atccgatccg gagttcggct ccgtcagccc ccaggcgccg agccactcgc
     4681 cactggcgag cttaggaata tacttgcgcc gctgctcttc agttcctgca agaaagatat
     4741 ggtttgtgca aagtgaatta tgtgccgcca caatgatccc gatggatcca tctacggcag
     4801 acagttcttc aatcgcagtc acatactcca catatcccag tccggagccg ccatattcaa
     4861 cggggaagat gatacccatc agtcccatct gtcctaactg ctttacaacg ccaacaggaa
     4921 attcgctggc ttcgtcccag cgcatcacgt tcggcgcaat ctcgcgctgg gcaaactcgc
     4981 ggatttcttt gcgcagttgt tgttgctctt ccgtaaggcc aaagggatag gttgctggtg
     5041 attgcaggtc cgcttccaca ggcatggatg actcccaaaa atcgcaatta tgagaaaacc
     5101 tgcaatcgta acacttttca gggagacttg tcgcctgcca ttccaatgcc tgccgtgcgg
     5161 ccactatcaa agacgaatac tttccagaaa atcccgcagg gcatttctaa tgcccgatat
     5221 caaaagaatg ccgcggtctt ctgcgctctc ccgcacgtag actcgtgcag tccccatcca
     5281 ggagtggaaa gtatccgggt tggaaagacc agtctggaga gaatggatat ccagcaaagg
     5341 catataaaat ggatatccgc gcagcgcttc ttcgcgaatt tcaggcagga accgttctgt
     5401 tgcgtttctg tgaaactctt ccggagcaga ggcgagcaaa gcgtaaatca gcgttcctga
     5461 gccagccaac gtagaaagcg catgttcgct tgcctctgtg ccaccagaga catgaaaaac
     5521 ggtgtacatc atgggccgga tcttctgccc cggatcaagt tcatggaagt cttcagcact
     5581 ccagagccgg ggaggctcgc ggtctggcca tactccaagc ccggtttccg ggatccattg
     5641 cgcaccatgt ttacgcacga ggtcggccgc attgccttca tgcaggtccg ctccctgaat
     5701 ccaggcggcg ggttgcagac tatgctcatg tgccaattta cgaagccagg gccttaattc
     5761 attgcgcaca aattccggag tttcttcgtg atcaggaaac cgcccagtct caaccagtgc
     5821 agcgtcgaat ggtcctggcg caggaggcga agaaacatgc aaggcttctt tcttcataac
     5881 ggatacacca taccttcgcc gtgcatctgc tcaagcaaat cggaaagtgg cgcatcgaaa
     5941 gccgggtctt tcagcgccag aaggatgcct ttatcatccc tgctttcgat gcaatatcca
     6001 tcgaatactt caaaccactg ctgtcgtatt tccggacctg catcaaagaa atttctgctc
     6061 gataacagag gcagtacaaa agatgcgttc ataaacgcag gatcgccttc tatattcttc
     6121 ccgaacacga tcttgctctt cttctgaaag gaatccagaa gagcataggt cgtctgcaaa
     6181 tgctgttgga tgtttgaccc gcgcgattcc aacattcttc ttactgaggg aggaagctta
     6241 agatcggggg ggcgcgtaat cgggtcttct ttcagccaga gaaataccat accgcccatg
     6301 ccgaagaaat gctgccgctg ctcctcatct tgtttccgca caaaccagta agtgtagtcc
     6361 ggaatgtgct tgtccattga gaaatccgcc tgcagcgaat ccttcctgag ctggtctgcc
     6421 tcgggaagtg gtggaagcgt ggccccgggt ttcaacccaa gtccggcaac cggaacaaag
     6481 tccaaggcat tcgacataaa atgtccggac ttttcagccg tgactgtttt gaattgcagg
     6541 aagttctcct gacgaaggtc ccgctctgcc atcagttttc tgaatcgtgt caggaagttc
     6601 atccaccatg tgcgcacgcg aaaactcatg gggaaactgt ctaccggcgc ccaatacagt
     6661 gacacatctt caagttcatg cgcccgcaga aaaggcttca attgtatgtc tcgttcaact
     6721 ggttccagac gccggcggcg gtctgtttgc gcaacttcca tactattcat ctgcgatgat
     6781 ctctatcgtg gtccggaatt ggtgcctcta cggctgaata tcatttgcgc caagaaactg
     6841 attgattgcg tgggctgctt ctggctgtcc ttgttggtct gaataagacc ccaacccgac
     6901 gcgatggcca tcgtccagaa caaatacgag gcgatatgcc gcagttgttg attccacagt
     6961 agcgtatttc acttgctgca aaggatatgt tgatgtgaca tgcgcccatc cgatatggat
     7021 tctcaataaa ccagcgtctt tatccaggac cgccgtagag ggaacagacg tggtccatcc
     7081 aagcaaagca aagcagacca gcaaaagcgc tcccataact ccagtagaac ggctgcgcgc
     7141 attgatgcca ctcagcagca gtaatagtcc gatgaccgca aaaacatagc acgtgactga
     7201 tacccacatc acgggagggt ttacgatcat cagccgtcca ttgccaaaat gttcgacttg
     7261 aaatcctgat gccaaaagag acaacatgaa actccgtgtt ctggaatcag aaaggcggta
     7321 ttactaattc gttcacatca attccggcat ttacatcaag aggcactccg tgcgcatagg
     7381 ccatggcaag tcctacctgg acccagtcaa tctttgacat aagccctttg atcttgacgg
     7441 aaagcggctc agcttccaga ttgattgctg ccgtattttt gttgcggaca aacgaatcct
     7501 gcagatagat agaggcccac tggagcttca gcagcgctgt tgtgtcaata ttcaggccga
     7561 caacgccaat cttgtaccac gcatgctcaa aatgctcctg aaaatactcg agcacattat
     7621 tttcgtggcg ctggtatgtg gtcggctggt tgacgtggct tggttgcgaa tgttgctggg
     7681 tcagcgtatg cacatatgtc tggttggatg gcgcaatatt tgtgcggttg tgcacgccaa
     7741 taaatgtttc gttggttttt cctacaacgg taagcttctg gtcggactga agcgtctcct
     7801 gccggtttcc cttgactgtc aattgataat tagcgtcaat aaactctgtg taattaccag
     7861 caaagtgttg ccactcattt cccttcacca gacatgtatc taccccattt gtgcccgcta
     7921 acgccgtttg agaagtaatc gcgccgtaat tcaatgagcc agcagctgga agtttgtggt
     7981 cagtgcctgg atcggcaaca gccagaggag taagcaccgt tccattaaat gcactcatac
     8041 gtgagcaacc tcgtccagag tgacttacag aaacaatgaa attctatcga ggcgcattac
     8101 ctgcaaacat gaaaaatctg ttaatcaata aagggtgttt tctgatggct tatagtcttc
     8161 gccaaatttg ttcttgtgcg caagcacatc agagctgcga gtaatatgga tgtctccgag
     8221 gtcggttcgc ggagaacgaa catgcacagc ccgggtaaac gcctgaagct gttgtgtatc
     8281 agcaccttcg gcccaaacat gcagttcgta gtcgccctct ggaacattgt gtatgaccac
     8341 actcccgtct gctgcggatt cggcaacata gggtgttgcc aatgcaatga caacggcgct
     8401 catctccggg tggatattgc agaagatata ggagacccct tcatgatcga agcgtacggc
     8461 tttggtactg cctgcttcgt ataaccccaa atcgaaacgc ttcccgttaa attgagaaaa
     8521 gacgttatga aagaagggat cgagattggg aaaatccact gtgcttccaa gcggtatcac
     8581 cagaagatgc ggggtaaatt tcttgtcctt ctgcacgagc ctgaagtggg acggctttgc
     8641 cgctgagacc agatagggag gctgtagcca gacaacaacg ccggtatttg aagccttttt
     8701 gccgccagac acaacaacct gcgcatgcgc aatgacttct gcacaatgtg cagtgcccac
     8761 gctcataaga gtgcagctta gaaccaacgg taagcagtgc ttgcgaaaca tagtgcactt
     8821 caaaatagat atcccgctgc caatcccaca ctctggcttg cattcaggct tcccacaata
     8881 ggccagctat gaatattccg gtattcggca gaaaacagaa gatatgtttt aggccgaaaa
     8941 atgaaattcg ccagcagtgt gcggtttctt gcaagatttc catagttgtc ctggtttgac
     9001 gcaaattgtg actcacgcag ttcctgggcg aagcctccgt cctgcccggc cgcaaaattc
     9061 gcttcaagat tgcgcaaaag tctgaatttc aattgcgccc atccgccttc cgtatcaagc
     9121 ccctggaagt gcgggtactc ggcgtagtta acatagtcct tataaacact cccgccaagc
     9181 ccgccaatcg cgcgccctcg atacagttcg ccagataatt ccagatgtga cgtaaacgga
     9241 acgctccagt ctgccgtcac tgcccatgca tccgcgtggt gcccataggg atacgattgc
     9301 ctgctgtaat agccgcccgc gccaatctcg atagtctggt caccggcatg cgtattccaa
     9361 ctcattctgg attcgtatcc aggctgcctg cttcgctctc cggcaccggg cccacgtaca
     9421 gcatagcttt gtggaggccc gggcgaagca gggtctatca atgcaaattc aaatcctgct
     9481 ttgctgccat gaacgggcag agtgtgtttc cacgccagct gcggtgacca tgtccacaga
     9541 ttgcccgacc atgcaagcgc cggttcacct acgctaagat acgacgttgg attcaaggga
     9601 gaaatcacag gagcatccag catgactgca agtgaatcgt gcccccagtc cagacgcgca
     9661 tgcgctgtgc gaagccgtag gattcccgcg ctcgtattgt agtcggcata cggaatgcct
     9721 ccaaagaagt ccactctgag gtcggcggca gagtgtgcgc cccacagggt gggaccttcg
     9781 gcattcagcc cgagaatgga ctgtcgtgct gttgctgtca gacttccatg cgcattatca
     9841 ggcatctttg ctaccgcaat gatgggcaaa tccgcatcgt ccacagcacc gtcgttcagg
     9901 agagacgtca tcagcaccat gcccgaaacc tgcagaggat atttcgacat gctttctact
     9961 tttgtctgat cgtgctgctt tacttcagat tggacgatat cgctttgctc ctgtaactgc
    10021 tgcacgctgc tgcgcaaatc ggaaacaatc tgtgccgatt cgcctgcatt gttttctgcc
    10081 aactgcatgc gcatatgctg caattcaacg cgcaattgct ggatttcttt ctgtgaatca
    10141 tcgagttgat gacgcatccc atcgagctgg actaaaacct ggtccagtcg tttttccagt
    10201 tgtacagttg ctgcgtcccc acttacaggt tgttgcgcta ttgctgcacc ggcaagaaac
    10261 aagccagtaa acatgcatgc ccattttgag gtattacggt acattgttcc atctcctcga
    10321 tgactcagtt gtgcggcaac aatgttgcgg gatgcttttc ctgtgcggac tccgcgatct
    10381 tattcagcat tgctttagaa agagaaaggt taaatatggt ccgcccaggt tgtgactcta
    10441 cgagagtaac gctgccgcca tgttcttctg caatgcgatt ggcaatcgta agccctaatc
    10501 cgatacctgc ctgtttgcct tcgctcacaa aaggctggaa caaagtaacg cgaatggact
    10561 cgggtacccc agggccattg tcctgaatgc tgatattgat ccactctggt gtttctgcaa
    10621 tcgcaagatg gactgcggga atgtccgcac cgtttttcgc agcctgacag gcattcaaca
    10681 acaggttgta aatggcacgc tcaattttcc tcacgtccag ccatacttca atctgcggca
    10741 gtgcgtccca tgtaatggca atcccctgcg tgtcccgatg tgcgcggacg agggtaatgg
    10801 cgcgctcagc tataagtgcc agcgattcgt agccaggctg caatgactga ccggtacggc
    10861 tgaagatcag caatgaatca atcaactcca gcatgccctc tacacccaaa cgcacttctg
    10921 caagcagttc tgcgcgttca gcagcattcg tggattcgta tccgaggaac tctgcatttg
    10981 catagactgc ggaaagataa tgccgtaagt catgagaaat agaactggcc attcttccaa
    11041 tcgttgcaag gcgttcagcc tcaatcagct ctttgcgcgc ctgttccagt tgcatcctca
    11101 tgcggtcgaa cgcaacgccg agttcctgca attcccttgc cccgcggttc tggaactgaa
    11161 agtgaaaatc acctgctcca agcgctcgcg cccctgcaac cagggaatcc agcgggcgag
    11221 taatcgtgct ggatatgtag acagcaatga cagcacccgc aaacagcaca caaaggccaa
    11281 gcacaatcag caattggtcc agacggcgaa ggtactgcgc tgcttcgtca tacgacttca
    11341 aaacaaccaa ctgcacgtgg ttgtcattgt tttgtgcaag aggaactgta gcgccgacat
    11401 aatgctcgcg ccgaagccag agatcagtag ctgtctcctg tgcatggaag gccccatcgc
    11461 catactttgt gaactcttgc cagcgtggct gatcgagtgt tgttgccaca atattgttat
    11521 tggctttgaa tatcacctca gcagcagagg cctcgctgac ctcacgtgcc accctgctat
    11581 ctatcagata tccaacggca acatatccaa gcagtgttcc cgtctgcggc ggaccaagat
    11641 acagcggctg agacgaaact tcgtataaac gcccattgtt aagaacatag tttggctctt
    11701 ccggcagaaa gaccctgttc gcttccgcag aagcaatgcg cggaatgccc ctggaaggac
    11761 cattggtctc ataaagcgca atcaggtgac tgttgcggtc cgccagcgcg aaaaaatcgc
    11821 tgccgctgac gcgaaaaaac tccgctccag catcctggat ggtcagtgta tccggtgctg
    11881 tcatcagaga cttcaagctg ggcagatcgg ccaaaagagc ggcttctcgc tgtaacatat
    11941 tgcgccgctg tacctgcatg ttccggaatg tcatgatcga acggtgaaga tcagagacga
    12001 tgtctctgcg tatctgcttt tcaagaatgg tgtgaacaat gagcagactc agcgcggtca
    12061 atccaaaaga aacagaaaaa aggcagagca gcagcaacgt gcgcatgcgc attgcggtct
    12121 aggcctcctt tctggttact tctccggcga ttgcacaaac ttatatccaa ctccatgaac
    12181 ggtgcagaaa tgtactgggt tcgcaggatc ggtctctaat ttctggcgca gcttgagaat
    12241 ctgattatct actgtgcgcg tagttggata gaaattgtag ccccatacat cgttcaaaag
    12301 ctcttcgcgc gtgagcaccc gttcaggatt gtcgagaaag aaccgcagaa gcttgaattc
    12361 atgcgcagta agtgttacac gttttccgct gcgggtgacc tgcattcggg cgaaatctac
    12421 ctgtacttcg ccaaaatgca ttgaaggtga agtccgcgtg cgcttattac ggcgcatggc
    12481 agcctgtacg cgcgcaagaa gctcgcgcgg gctgaacggt ttggtcacat aatcatctgc
    12541 gccaatctcc agcaacagca ccttgtctgc aacctctgct atcgcactga gtacgattac
    12601 cggcgtatcg ggcgaagact gtttcatggc gcggcacagc tcgcggcccg agacaccggg
    12661 aagcatcaag tccagaatga cggcatccgg cgttccactg gaaaacgcct ctagcccgtg
    12721 cgacccatcc gagacaccct tcacttcata tccctctgag gcgaataaac ggtgcagggc
    12781 cttttgaatg cgcgtatcat cctcgatgac cagaatcgta ttcatggctt acatctgcgt
    12841 cctcttattg tctgctaatt caagggttta tagaagcctt tccaggaaaa gtgacaaaag
    12901 agtgacattt tcatgacata cctatcaccg actgctcgat aattgctatg agcagtcata
    12961 gaagagttct acctatggca tagctcattt atcagcactc ctgcaaaaca agtgtaattg
    13021 cccgccttta tgcctattcc gaatttcttt atcgtgggag cacccaaagc tggaacagac
    13081 gcgctcttct acgaccttga acaacatcct gaaatttata tgagcccgct caaagagcca
    13141 tgcttctttt caagcgaaat ccgccctgag aattttcatc cttctctgca gccgcaaata
    13201 gaagcatcgc gggatgagac ccgcaggtac ctggatgcgg gcatgcccgg caaacgattt
    13261 ggcggaatca tctcgacatg ggaggactac ctcaagctct tctcgtgcgt gactcacgaa
    13321 aaagcaattg gagaaggaag cgtttgctat ctctggtcag agacggccgc cgcagccatc
    13381 gcctctgctg tcccacacgc gcgcattgtt attgtcctta tggatccggc ggagcgtgcc
    13441 tttcatcagt atctgaaaag cgtatccgat gggactgtca ggcatagttt ccgtaagcat
    13501 cttcaaatgg ccatgcagtc tggtccggag ctaggcgtat atcatccctt tcttgctttt
    13561 gggaattact ccgaccaggt aaaacgttac atggaccagt tccctacaca tcaactctat
    13621 atttcacttt atgaagagct gcgcggcgat tatgcaggct ggctttcacg cttattgtta
    13681 tttttgggag tcaatccggc atttgttccc gcgcattcag aggtcccatc tacgccgcat
    13741 cttctca
//
LOCUS       DASRBV010000042         9719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_407080, whole genome shotgun sequence.
ACCESSION   DASRBV010000042 DASRBV010000000
VERSION     DASRBV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407080"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFA02_01885"
     CDS             complement(<1..884)
                     /locus_tag="VFA02_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HZU08626.1"
                     /translation="MRDKQLLSAFEDKKHEPQMSQNGKDKEGVVPVRKGLTLEEVRAR
                     LKGKTGRKYWRSLEELADTPEFHEMLGREFPQHASEWIDSVSRRGFLKMAGASLALAG
                     LAGCTKQPDEPIYPYVKAPEDLVLGKPMYFATAFPFPTGAVPVLVKSDAFRPIKIDGN
                     PEHPYIKGGSDPITQATLLDLYDPDRSQHVVYRGETRTWGAFQAAWRAKLADKKPSGG
                     QGIYILTGTITSPTLAAQWKKAQAAYPNAKLVQYDPVNRDSAYTASKAAFGDYLDAQY
                     KLDAADVILSLDADFLSGA"
     gene            complement(881..1759)
                     /locus_tag="VFA02_01890"
     CDS             complement(881..1759)
                     /locus_tag="VFA02_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZU08627.1"
                     /translation="MAQIFDRSSNALARMSLVLTGLIVIALGITLDEWQRSPWWTRQG
                     QRADQPVPFSHKHHVQGLGLQCQYCHTSVENSSYAGIPPTKTCMNCHAQIWTNAELLE
                     PVRQSWATNESIVWTKVHDLPDYVYFNHEIHVNKGIGCSSCHGRVDEMPIMYQQNTLQ
                     MEWCLNCHRNPAVNLRPTSEIYNMAWTGPSELKPVWCAVKQQAQPGVPTGQDVTCTES
                     DPSGKGGPEIAALQLPAGLHAQAAASDAGGGATAVPAVLNYQKFTSQEELGKFLIGQY
                     HIRNPRELSSCEVCHR"
     gene            complement(2115..2699)
                     /locus_tag="VFA02_01895"
     CDS             complement(2115..2699)
                     /locus_tag="VFA02_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU08628.1"
                     /translation="MARTRSESAHRKVIGAALALFAERGIDATSMDAIAGASGVSKAT
                     IYKHWRDKEALFLEAFSHLHGLDKEPPVFDSGDLRADLIAQLSYQPAAHRERMREKIM
                     PHLIAYSARNPAFGKQWRELVLERSRRRLREMIERSIQRGKLKPIPPEIAVPLLLGPM
                     LYQYIMLKQLDGKSVPDLVSYVVDTFLAAYGIRA"
     gene            complement(2703..3449)
                     /locus_tag="VFA02_01900"
     CDS             complement(2703..3449)
                     /locus_tag="VFA02_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08629.1"
                     /translation="MNAIYILWLRELKRYVRSRVQVVASLGQPVLYLLALGFGFGPVF
                     AQAGRGSYLQFMAPGVVGMTVLFSAVFSGMAILWDRQFGFLKETLVAPVSRLQIMAGR
                     TLGGATVAVIQGSLVFLICLLAGFRPQGWIAVGETALFMALVAVVFAALGTACGSLIQ
                     DMQGFQIIMNFMVLPIFFLSGALYPLSNLPRAMTIITHIDPLTYGIDGLRGALIGAWH
                     FVPALDVLVLGAVATVFLGLGAYLFSRIQL"
     gene            complement(3454..4239)
                     /locus_tag="VFA02_01905"
     CDS             complement(3454..4239)
                     /locus_tag="VFA02_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU08630.1"
                     /translation="MIRVQNLVKTFGEFTAVNDISFDVRQGEIFAFLGPNGAGKTTTI
                     KMLTTLLKPTSGTIELDGLNPSQKQKEARQRFGIVFQDPSLDGEQTAYENMELHGILY
                     RVPRKVRAERIERLLKQFELWDRRDDYVKQFSGGMKRRLEIARGLLHTPKILFLDEPT
                     LGLDPQSRNQLWTHVKKMNETEKTTVFLTTHYMDEADRVAHRIAIIDHGRIIAQGTAA
                     ELKERTNTDSLEGAFLALTGESLRDESATSTDAMRVMARMWRR"
     gene            4370..5542
                     /locus_tag="VFA02_01910"
     CDS             4370..5542
                     /locus_tag="VFA02_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08631.1"
                     /translation="MAATTSANPELAYAKEKSTQIPWYLWCAALAVTSVTIGAHWDVS
                     WHRSIGRDTFWTPAHMAIYACGVLAGICCSYLILNTTFRRSPEFVAASVSVLGFRAPL
                     GAFLAAWGGVAMLTSAPFDNWWHNAYGLDVKIVSPPHTLLMIGIFAVEIGALFLIAAA
                     MNRADGVLSRKLQRLLLYLGGLMLVLTMFFRMEYTWDVRLHSSGAYIATAIGVPTYFA
                     LFWQCSRNRWAATWMGAFYTVFGIGLVLVLPLFSAEPKLGPVYEPVHQFIPPPFPLLL
                     IVPAFFMDLLLRTTKSWKPWLVSLLAGPLFVLSFVAVEWPFASFLMTGAAKNRFFGTG
                     YLDFSTPPWSHDALRQFLHPEHGFVLWKGLAVAMLYAALSTWLGMAMGNWIRKVQR"
     gene            5543..7024
                     /locus_tag="VFA02_01915"
     CDS             5543..7024
                     /locus_tag="VFA02_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08632.1"
                     /translation="MRCLKQKLWLTGLLVLLCVLPARAHVGSPDVYAQGDAGPYKLFV
                     VVRPPLVIPGVAEIQVRAQTPGVESITAAPMLLTGEASKHPPVPDTLTKSPGDPQFFT
                     GKLWIMATGSWQVRFVAHGDQGQGVFSVPLPATALGTRKMSLGLGFLLAVLGAVLVLG
                     IVGIIGAAAREAQLKPGEKVPAATRNRAWVAMAVTFVLLISAVVLGGMWWRAEAADYS
                     SYIYKPLQMQAVVQPDHVLELRLKDPGWLSARKLDDFVPDHNHLMHLYAIRWPAMDVV
                     YHVHPQQTAAGDFQLALPDMPAGTYRLYADVVHANGFPETLTSTVTVPEIHGKALSGD
                     DSMGKATPAGTENAMVCKLSDGYTMVWKRPAALKANAAEDFTFQLLDPQGQAPKDMAL
                     YMGMAGHAAFVKTDGTVFAHIHPSGTVSMAALMMAEAQNPSSHSQMMMPDMPGMDMSG
                     GNTPVSNTVSFPYGFPRPGNYRIFVQMKHGSVIETGTFDASVE"
     gene            complement(7042..7926)
                     /locus_tag="VFA02_01920"
     CDS             complement(7042..7926)
                     /locus_tag="VFA02_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08633.1"
                     /translation="MIFRRLMICLPVFALLAGAPLIAQVSYTSMPLDSGFGKLDTSQP
                     DVPVQQIIDRFTEKESEFAHAMENYTYERTVKVETIDDDGKTDGQYYQVTDITLDPTG
                     RKYEKVVFAPESTLTRIMMSPADFEDIEHRLPFVLTKEGAAQYDISYVGKQRVDEVDT
                     YVFDVKPKVIEKNKRYFQGRIWVDQKDLQIVVTNGKNVPDDLRKGHEDLSPPFATYRE
                     QIDGKYWFPVYTRGEGVLHFTAGTGYLSQDVHIRETVTYRNYKRFGTSVKITYDGQEV
                     GKSDDKQQQQPAPQKPQQ"
     gene            8075..9208
                     /gene="mraY"
                     /locus_tag="VFA02_01925"
     CDS             8075..9208
                     /gene="mraY"
                     /locus_tag="VFA02_01925"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZU08634.1"
                     /translation="MLYWLLYQKLFPYFRPFRIFRYLTFRTAFASLTALLIALIIGPY
                     VIEKLREFQIGQYIREEGPKEHQKKAGTPTMGGVLICIAILLPTLLWADLSNPFVWLV
                     MGSTFAFGAIGFADDYIKVVKRRNLGLTARAKMGYQILASLAISLALVYMQTEGIYST
                     KLMVPFVKWLKPDFAIEALRHVPHFGLLAFLPFIVFTVLVIVFSSNAVNLTDGLDGLA
                     IGCTIIAAGALTVLTYVSGHVVFADYLELQRMPLVGELSIFCGSMVGASIGFLWYNAH
                     PAEVFMGDVGSLALGGAIGTVAVAIKQELLLPFIGGIFVLEALSVILQVGSYKLRKKR
                     IFKMAPLHHHFELMGWSESKVIARFWIGALVFALLALTTLKLR"
     gene            9261..>9719
                     /locus_tag="VFA02_01930"
     CDS             9261..>9719
                     /locus_tag="VFA02_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZU08635.1"
                     /translation="MEFKGKKVLIVGLGKSGLAAALFLRRRGAQVTVSDIRSAEQLGK
                     DIPALLEEGIAVEAGGHGLLTFRRQDLIVVSPGVPLNTPELVQVKTFGLPIIGELELA
                     SRFLKGKTLAITGSNGKTTTTSLTGEILKAGGLNTQVGGNIGVPVIALVDE"
BASE COUNT         1984 a         2830 c         2666 g         2239 t
ORIGIN      
        1 gcgccggaga ggaagtctgc atcgagcgac agaattacat ctgccgcatc gagcttatac
       61 tgcgcatcaa gataatcacc gaaagcggcc ttcgacgccg tatacgccga atcgcggttc
      121 accgggtcat actgcaccag cttcgcattt ggataggccg cctgcgcctt cttccactgg
      181 gctgcgagtg ttggcgaggt aatggtgccg gtcaggatgt aaattccctg cccgcccgac
      241 ggtttcttgt ctgccagctt tgcgcgccac gccgcctgga aggcccccca tgtgcgcgtt
      301 tctccgcggt aaacaacatg ctgtgagcgg tcaggatcgt ataggtcaag caaggtggcc
      361 tgcgtaatcg ggtcagatcc gcctttgata tacgggtgct cgggattgcc atcaatcttg
      421 attgggcgga acgcatcgct cttcaccagc actgggactg cgcctgtcgg gaagggaaat
      481 gccgtggcga aatacatcgg cttgcccaga accaggtctt ccggggcctt cacataggga
      541 taaatcggct cgtccggctg cttggtgcaa cctgccagtc cggccagcgc cagcgatgcc
      601 cctgccattt tcagaaagcc gcgccgggaa accgaatcta tccactctga agcatgctga
      661 ggaaactcgc gccccagcat ctcgtggaat tccggggtat cggccagctc ttccaggctg
      721 cgccagtact ttctgcccgt cttgcctttg agcctggcgc ggacctcttc cagcgtcagc
      781 cccttcctga ctggcaccac tccctctttg tcttttccgt tctggctcat ttgtggctcg
      841 tgtttcttgt cttcaaaagc ggaaagaagc tgcttgtccc tcatcggtgg cagacctcgc
      901 agctcgacaa ctcacgcgga ttgcgtatgt ggtattgccc aatcaggaac ttgcccaact
      961 cttcctgact ggtgaatttc tggtaattca gcactgcggg aacggccgtt gctcccccgc
     1021 ccgcatcgct ggctgcggcc tgcgcatgca gccccgcggg caactggaga gcagcaatct
     1081 ccggcccacc cttcccgctg ggatcgctct ccgtgcaggt cacgtcctgc cctgtcggga
     1141 cgcccggttg cgcctgttgc tttaccgcgc accacaccgg cttcagctcg ctcggaccgg
     1201 tccaggccat gttgtagatc tcgctcgtcg gccgcaggtt cactgccgga ttgcgatggc
     1261 agttcagaca ccactccatc tgcagcgtgt tctgttgata catgatcggc atctcatcca
     1321 ccctgccgtg gcaactcgaa cagccgatgc ctttgttcac gtggatttca tgattgaagt
     1381 aaacgtagtc cggcaggtcg tggaccttcg tccacacaat ggattcgttt gtcgcccagc
     1441 tttgccgcac aggctccagc aactcggcgt tggtccagat ctgcgcgtgg cagttcatgc
     1501 acgtcttcgt cggtggaatc ccggcgtagc tcgaattctc taccgacgtg tgacagtact
     1561 ggcactgaag cccgagtccc tgcacatggt gtttgtggct gaaaggcaca ggctggtcgg
     1621 ccctctgtcc ctgacgggtc caccatggcg atctttgcca ttcatccagc gtgatgccca
     1681 aagcgatgac aatcagtccc gttaagacca ggctcatgcg cgccagtgcg tttgagctgc
     1741 ggtcaaaaat ctgcgccatg tatgttcctg tttcctcttc agtccctgag aatcgcaggg
     1801 gttaagggtt tatgcccgta aaaaattttt cgtgcaccga aatgtatcta atttttttga
     1861 agcaacacta tagtggcgga aaggtattct tcgcaagtct ctcaggtgac aatacgccga
     1921 aaagtgctca aatcaacacc ataacggagc acagaacaca gggcgggccg caggacaata
     1981 tatagtccag ggctttggcg tacaaacgaa gcgcacaaca ctcagcgcat tttcactcgc
     2041 tgtgttcaaa tactgctcat aaattaaatt tgtcatcctg aaggcgcaac cagctcaggg
     2101 cctgccatca acccttacgc tcgaatgcca tatgcagcca gaaacgtgtc caccacatac
     2161 gaaaccaggt ctgggacaga tttcccgtcg agctgtttca gcatgatgta ttggtaaagc
     2221 atggggccaa gcagaagagg aacggcgatt tccggcggaa tgggtttgag ctttccgcgt
     2281 tgtatgctcc gctcgatcat ctcccgcaat ctacggcggg accgttccag aacaagttcg
     2341 cgccactgtt tcccaaaggc agggtttcgc gctgaatagg cgataaggtg cggcatgatt
     2401 ttctcacgca tcctttcgcg gtgcgcggcg ggctgataac tcagttgggc gataaggtcg
     2461 gcgcgcaggt caccggaatc gaatacgggc ggctctttgt ccagtccatg cagatgactg
     2521 aaggcctcca gaaacagggc ttctttgtcc cgccagtgtt tgtaaatagt agctttgctg
     2581 acgccggagg ccccggctat ggcatccata ctggttgcat ctatgccgcg ctcggcaaac
     2641 agcgcgagcg cggctccgat gaccttgcga tgcgcggatt cgcttctggt ccttgccatc
     2701 gcttacaact ggatcctcga aaagagataa gcacccagtc cgaggaagac tgtggcgact
     2761 gctcccagca caagcacatc gagcgccgga acgaagtgcc atgccccaat caatgcgccg
     2821 cgcagcccgt caatgccata cgtcagcggg tctatgtgcg tgatgatggt catcgcgcgc
     2881 ggcaggtttg acagtggata cagcgccccc gacaaaaaga aaatcggcag caccataaag
     2941 ttcatgatga tctgaaagcc ctgcatgtcc tgaatgagag agccgcaggc cgtccccaat
     3001 gcagcgaaga ccaccgccac cagcgccatg aacagcgccg tttctccaac cgcaatccag
     3061 ccctgtggac gaaagccggc cagcagacaa atcaggaaaa ccaacgaacc ctggatcacg
     3121 gcaaccgtcg caccgcccag ggtccgcccg gccatgattt gcagccgcga aacaggagcc
     3181 accagcgtct ccttcagaaa gccgaactgg cggtcccaga gaattgccat gccggagaag
     3241 accgcactga agagcacggt cattccaacc actcctggcg ccatgaactg caggtagcta
     3301 ccgcgcccgg cctgggcgaa gaccgggcca aagccgaaac ccaatgccag cagatacaac
     3361 accggctgcc ccagcgatgc tacaacctgc acacgcgagc ggacatagcg cttcagctcg
     3421 cgcagccaaa ggatgtaaat ggcgttcatg ctgctacctc ctccacattc tcgccatcac
     3481 gcgcatggca tcggttgagg tggcgctttc atcgcgcaga gactcacccg taagcgccag
     3541 gaatgcgcct tccagcgaat ctgtgttggt ccgctctttc agttcggcgg ccgtaccctg
     3601 ggctatgatc ctgccgtggt caatgatggc gatgcggtgt gccacacggt cggcctcatc
     3661 catgtaatgc gtggtaagaa agactgtggt cttctccgtt tcattcatct ttttcacgtg
     3721 cgtccagagc tgattacggc tttgcggatc aagccccagc gtcggctcat cgagaaaaag
     3781 aatctttgga gtatgcagaa gcccgcgggc aatctccagc cgccgcttca ttccgccaga
     3841 gaattgcttt acataatcat cgcggcggtc ccatagctcg aattgcttca gtagcctttc
     3901 aatacgctcc gcgcgcactt tacgcggaac gcgatagagg atgccatgca gttccatgtt
     3961 ttcataagcg gtctgctcgc catccaggct gggatcctga aacacaatcc cgaagcgctg
     4021 tcttgcttcc ttttgtttct ggctgggatt caatccatcc agctcaatgg tgccgctggt
     4081 gggcttgagc agcgtcgtca gcattttgat ggtggtggtc tttcccgccc cattcgggcc
     4141 gaggaaggcg aatatctcgc cctgccggac atcgaaagag atgtcattca cggcggtgaa
     4201 ttcgccaaag gttttcacca gattttgcac acggatcata aagttggttt ctccgtaaaa
     4261 acaatactga acagtttagt ttagttttgt caagacgcct gacgcccgga cgaaggcacc
     4321 ttccagtcgt agatcacacc aaggcgtatc ctgaaacaga ggggatgcga tggcggcaac
     4381 aacaagcgcg aatcctgagc tggcttatgc aaaagagaag agcacgcaga ttccgtggta
     4441 cctgtggtgc gcagcgctgg ccgtaacttc agtgaccatc ggagcgcact gggatgtctc
     4501 ctggcaccgc tccattggcc gcgatacctt ctggacgccg gcgcacatgg ccatctatgc
     4561 ctgcggagtt cttgccggca tctgctgcag ctatcttatt ctgaacacca cattccgccg
     4621 ctcgcccgaa ttcgttgcag catccgtaag cgtgcttgga ttccgcgccc cactgggagc
     4681 atttctggca gcctggggcg gcgtggctat gctcacctct gcaccattcg ataactggtg
     4741 gcataacgcc tacggcctcg atgtaaaaat cgtcagccct ccacatactc tgctgatgat
     4801 cggcatcttc gccgtcgaaa tcggcgcgtt gtttctgatt gccgccgcca tgaaccgcgc
     4861 agacggcgtg ctttccagaa aattgcagcg tctgctgttg tatctgggtg gattgatgct
     4921 ggtgctcacc atgttcttcc gtatggagta cacgtgggat gtccggctgc actcctccgg
     4981 tgcctatata gcaaccgcga ttggcgtacc cacatacttt gcccttttct ggcaatgctc
     5041 acggaaccgc tgggcagcaa cgtggatggg cgccttttac acggtgtttg gaattggcct
     5101 ggttctggtc ctgccgctct tttcagccga gcctaagctg gggccggtct atgaaccggt
     5161 gcatcagttt attccacctc cgtttccatt gctgttgatt gtcccagcat tcttcatgga
     5221 tctgctgctg cgaacaacga aaagctggaa gccatggctg gtttcactgc tggcaggccc
     5281 actgtttgtg ctttcctttg tcgctgttga atggccgttt gccagttttc tgatgaccgg
     5341 tgcggccaaa aaccggttct tcgggaccgg atatctcgat ttttccaccc cgccgtggtc
     5401 gcatgatgct ttgcgacagt ttctgcatcc ggagcacggc tttgtgctgt ggaaaggcct
     5461 ggctgtcgcc atgctttatg ccgccctgag cacctggctg ggcatggcaa tgggcaactg
     5521 gatacgaaag gtacagaggt aaatgcgctg tttgaagcag aagctgtggc tcacaggttt
     5581 gcttgttctg ctttgtgttt tgcctgcacg ggctcacgtg ggcagtcccg atgtctacgc
     5641 gcagggcgat gccggcccat acaaattatt tgtcgtcgtg cggcccccgc ttgtcattcc
     5701 aggagtggcg gaaatccagg tgcgtgcgca gacaccgggc gttgagagca ttacagccgc
     5761 tcccatgctg ctgacgggcg aggcgtcgaa gcatccgcct gttccggaca cgctgacgaa
     5821 gtcgcccggc gatccgcaat ttttcaccgg aaagctctgg atcatggcca ccggatcatg
     5881 gcaggtgcgc tttgtcgccc atggggacca ggggcagggc gtattttccg tgccgcttcc
     5941 tgcaacggcg ctgggtacgc gcaagatgtc cctgggcctg gggtttctgc ttgccgtctt
     6001 aggcgccgta ctggtcctcg gaattgtcgg cattatcggg gcagcggcgc gtgaggcgca
     6061 actgaagccg ggagagaaag ttcccgcggc aacaagaaac cgcgcctggg tggccatggc
     6121 agtcacattc gtcctgctca tttcggcagt tgtgcttggc ggcatgtggt ggcgcgccga
     6181 ggccgcggat tactcgtcct atatctataa gccgttacaa atgcaggcag tcgtgcagcc
     6241 ggaccatgtt ctcgaactcc ggctgaaaga cccgggctgg ctctctgcgc gcaagctcga
     6301 cgactttgtg cctgaccaca atcatctgat gcatctctat gccatccgct ggcccgcaat
     6361 ggatgtggtc taccacgttc atccgcagca gaccgccgca ggagactttc agcttgcgct
     6421 gccggacatg cctgccggca cctatcgttt gtacgctgac gtggtgcatg cgaacggctt
     6481 tccggagacg ctgacttcaa ccgtgactgt gccggaaatc cacgggaagg ccctgtccgg
     6541 cgatgattcc atggggaagg caacgccggc cggcacggaa aacgctatgg tctgcaagct
     6601 gtccgacgga tacacgatgg tctggaagcg tcccgcggca ttaaaggcaa atgccgcgga
     6661 agactttacc tttcagctgc tcgatccgca ggggcaggcc ccgaaggaca tggcccttta
     6721 tatgggaatg gccggacacg ccgcctttgt caaaaccgac ggtacagtct ttgcgcatat
     6781 ccatccatcg ggaacggtct cgatggctgc gctgatgatg gcggaagcgc aaaatccgtc
     6841 cagccactca caaatgatga tgcccgacat gccgggaatg gacatgagcg gcggcaatac
     6901 acccgtatcg aatacagtca gctttcccta tggatttccc cggccgggaa actatcgcat
     6961 cttcgtgcag atgaagcatg ggagtgtgat tgagacaggc acatttgatg cttcagtaga
     7021 gtaaactctg tttcgtctgt tttactgctg tggtttttgc ggagcaggct gttgttgctg
     7081 cttgtcatcg cttttgccca cttcctgtcc atcgtaagtg atctttacag acgtcccgaa
     7141 gcgtttgtaa tttctatatg tcaccgtttc acggatgtgg acgtcctggc tgagatatcc
     7201 ggtgcccgca gtaaagtgca ggacaccttc tccccgcgta tacaccggga accagtattt
     7261 tccgtcaatc tgctcgcggt aggtagcgaa gggcggagac aggtcttcgt ggcctttgcg
     7321 caaatcatca ggcacgtttt tcccgtttgt aacaacaatc tgtaaatctt tctggtctac
     7381 ccagatacgt ccctgaaaat agcgcttgtt tttctcgatc accttcggct taacgtcgaa
     7441 gacatacgtg tctacttcat ctactctttg tttgcctacg taggaaatgt catattgcgc
     7501 tgcgccttct ttcgtgagca caaagggcag gcggtgctcg atgtcctcga aatcggcagg
     7561 gctcatcata atccgcgtca gcgtgctttc cggagcgaac acgaccttct catatttccg
     7621 gcccgtggga tccagcgtaa tgtccgttac ctgataatat tgaccgtcgg tcttgccgtc
     7681 atcgtcaatg gtttcgacct taacggtgcg ctcgtaggta tagttctcca tggcatgggc
     7741 aaattccgac tctttttcag tgaagcgatc aatgatctgc tgcaccggaa catcgggctg
     7801 cgaggtatcg agtttgccaa agcctgagtc aagcggcatt gaggtataag agacctgcgc
     7861 gataagcggc gctccggcaa gcaacgcaaa gaccggcagg caaatcatca agcggcggaa
     7921 gatcacagga cactccaact actaatagga tagacgcgaa acggagcaaa agaggcgcgt
     7981 gcatccggtc ccggcgaatt ggtgaaatgt tgaggataca caggccgcaa gcctgtaaga
     8041 tgaagccagt atcccattgc gcgccggagg aagtttgctc tactggcttc tttatcagaa
     8101 actttttcct tacttcagac cctttcgcat cttccggtat ctgacgtttc gcacggcctt
     8161 tgcctccctg acggccctgc tgatcgcgct catcatcggg ccgtatgtga ttgaaaaact
     8221 gcgcgaattc cagattggcc agtacatccg cgaagaaggc ccaaaggagc accagaagaa
     8281 ggccggaacg cccacgatgg gcggcgtgct catctgcatc gcaattctgc tgccaacgct
     8341 gctctgggca gacttgtcga atccatttgt ctggctcgtc atgggctcca ccttcgcctt
     8401 cggagccatt ggctttgccg acgactatat caaagtagtc aagcgccgca atcttggcct
     8461 cacggcacga gcaaagatgg gctatcagat tctggccagt ctcgccatct cccttgcgct
     8521 cgtctacatg cagacagagg gcatctactc caccaaactg atggtacctt ttgtaaagtg
     8581 gctcaagccg gacttcgcca ttgaggccct ccggcatgtt ccgcacttcg gtctgctggc
     8641 ctttttgccg tttattgttt tcaccgtgct ggtgattgtc ttctcaagca acgccgtcaa
     8701 ccttaccgac ggcctcgacg gcctcgctat cggctgcacc atcatcgccg ccggcgcact
     8761 cactgtcctg acctacgtca gcgggcacgt tgtttttgcc gattacctcg aactgcaacg
     8821 aatgccgctc gtcggcgagc tttccatctt ctgcggctcg atggtcgggg ccagtatcgg
     8881 cttcctctgg tataacgcgc atccggcgga ggtctttatg ggcgacgtcg gctcactggc
     8941 gctgggcggc gcaattggta ctgtcgccgt cgccattaag caagagctgc tgctgccctt
     9001 catcggcgga atcttcgtgc tggaggcgct gtcggtcatc ctgcaggtgg gcagctacaa
     9061 gctgcgcaag aagcgtatct tcaagatggc cccgctgcac catcacttcg agctaatggg
     9121 ctggtcggag tcaaaagtca ttgcccgctt ctggattggc gctttggtgt ttgcactctt
     9181 ggcgctcaca acattaaagc tgcgctgaac acgtattcca catgacacaa tggcttgtca
     9241 ggccggaaaa tcataagcat atggagttca aaggcaaaaa ggttttgatc gtcggactgg
     9301 gcaagtccgg cctggcggct gccctctttc tgcgccgtcg cggagcacag gtcacggttt
     9361 ccgatattcg cagcgctgag caattgggca aggacatccc cgcgctcctg gaagaaggaa
     9421 ttgccgtcga agccggagga catggcctgc ttaccttccg acgccaggac cttatcgtcg
     9481 ttagccccgg cgtgccgctc aatacgcccg aactggtgca ggtgaagacc tttggcctgc
     9541 ccatcatcgg tgaactggaa ctggcctcgc gttttctgaa gggcaaaacg ctcgcgatta
     9601 ccggatccaa tggcaagacg acgacaacat ctctcaccgg agagattctg aaggccggcg
     9661 gcctgaacac ccaggtcggc ggaaacatcg gcgtgcctgt catcgctctg gtagatgaa
//
LOCUS       DASRBV010000043         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_559080, whole genome shotgun sequence.
ACCESSION   DASRBV010000043 DASRBV010000000
VERSION     DASRBV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559080"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFA02_01935"
     CDS             <1..547
                     /locus_tag="VFA02_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU08636.1"
                     /translation="HSQFCYEAARSPRTWIYDPKLALGGPVGDVAIHCFDALRFVLAT
                     DVTSVSTLARKDAESGDVEAFAVLAFAFGNGAMGSVSVTTRAAYRSLVEVSGEAGVIV
                     CENGLTVDHPVDVVLYRQGKVAAHQRVSNADAYRRMLDSFSAAIEGRGAYLATGEDGL
                     HNQRVLDAAYESWRTGKAQNL"
     gene            complement(599..>3247)
                     /locus_tag="VFA02_01940"
     CDS             complement(599..>3247)
                     /locus_tag="VFA02_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08637.1"
                     /translation="LSSCLLSAQSTSNSGTITGTVTDPTGAVVPGAAVTIVNPVSKYE
                     RTVTTDKMGHFQFPNVPFNPYHLTVTMTGFSNATQDVEVNSVVPVTANVSMKLGEASN
                     TTVVVTGEDLVENDTTMHTDIDRGLMQKLPLESQSSSVSSLVTLASPGVAADSNGLFH
                     GLGDHASNSFSVDGQPISDQQSKVFSNQIPADSVQSLEVIDGAPPAEYGDKTSLVIKV
                     TTRSGQGLKRPTGSINTSYGTFGSVNGGFDLGYGGDNWGNFITANGLNTSRFLDPPEF
                     KVFHARGNEQNIFDRVDYNFTQADSIHLNLNYTRSWFQTPNAYDNLNILDQFGNSVGE
                     TDQKSKIETFNIAPTYTRLINNYSVFNFGAYARKDAYNYYPSRNPFADFSPTQSETVQ
                     QRRTLLNTGAHTDISYVRGAHNFKAGAVYEHTFLRENDALGIVNPTLNSPCLDANGNP
                     VIGFTDPSQCAAAGDQPNANFNSDLLPYDLTRSGGLYGFHGRADIKEIALYAEDQITA
                     GNWLFSLGMRGDLYNGLTRERQAEPRLGVSYSIKPTNTVLRVSYARTLETPFNENLVL
                     SSTGCADPVLSPLLSCTPGVIGKLTPGFRNEFHAGFQQAFGKYAVISGEYIWKYTHNA
                     FDFSVLGNTPITFPINWHNSKIPGFTLRADVPNFHNISAFVVMSSVAARFFPPQIAGA
                     GATVGQSGFPFRIDHDERFNQTTHIQYQLPWRKSLWYGFNWRYDSGLVAGSVPCYNVT
                     DPNSGCGGTSITLPNGRPGIDLSGLTADQQFQAGLACDGVRATPTSGFTACDANGLTS
                     ELVKIPAPGTENDDKNPPRIAPRNLFDMAMGDDNLFGGDHYKWGLRLTAVNVTNKYAL
                     YNFLSTFSGTHYVTPRTVTGEINFSF"
BASE COUNT          718 a          864 c          924 g          741 t
ORIGIN      
        1 gcattcgcaa ttctgttatg aagccgcgcg tagtccgcgt acctggatat acgaccccaa
       61 actcgccctc ggtggccccg tcggcgatgt cgccattcac tgcttcgatg ccctgcgctt
      121 tgtcctcgcg accgacgtca ccagcgtcag cacccttgca cgcaaagacg cagaatccgg
      181 cgacgtggaa gcattcgcag tgcttgcttt tgcctttgga aatggtgcga tgggctctgt
      241 ctccgtcaca acgcgcgctg cttatcgcag cctggtagag gtttccggag aagcaggcgt
      301 cattgtctgc gaaaacggcc tgaccgtaga ccatccagtt gatgttgtcc tgtatcgtca
      361 aggaaaagtc gccgcgcacc aacgcgtatc caacgcagac gcctaccgcc gcatgctcga
      421 tagtttttct gccgcaatag aaggccgcgg cgcgtatctg gcgacgggcg aagatggcct
      481 ccacaatcag cgcgtgctgg acgccgcgta tgagagctgg cgtactggaa aggcacagaa
      541 tctctgagag ccaccccaca caagccagcc atcggcttgt atggggcgcc cgcatctttc
      601 agaaagagaa gttgatttct cctgtcaccg tcctcggcgt cacataatgt gtaccgctga
      661 aggtggaaag aaagttgtag agggcgtatt tgttggtcac gttgacggcc gtcaggcgaa
      721 gtccccactt gtagtggtca ccaccaaaca ggttgtcatc gcccattgcc atatcaaaca
      781 ggttgcgcgg agcaatgcgc ggcgggttct tgtcgtcgtt ttccgtcccc ggggccggaa
      841 tctttaccag ctccgatgtc aaaccgttcg catcgcatgc ggtgaaacct gacgttggcg
      901 tcgcgcgcac gccatcacag gcaagcccgg cctggaattg ctgatcagcc gtcagcccgc
      961 tcagatcaat ccccggtcgt ccgttcggca gagtgatcga ggtgccgcca caaccgctgt
     1021 tcgggtcggt cacgttatag caaggcaccg aacctgcgac cagaccacta tcgtatctcc
     1081 agttgaagcc ataccacagg ctcttgcgcc atgggagctg gtactggata tgggtagtct
     1141 ggttgaacct ctcatcatga tcaatgcgga aggggaagcc gctctggccc actgtcgccc
     1201 ccgcgcctgc gatctgcggt gggaagaaac gtgcggccac agaggacatg acaacaaacg
     1261 cactgatatt gtgaaaattc ggcacatcgg cccgcaacgt gaaaccgggg atctttgagt
     1321 tgtgccagtt gatgggaaaa gtaatcggcg tattgcccag tacgctgaaa tcaaaggcat
     1381 tatgcgtata tttccagata tactcgccgc tgatcactgc atatttgcca aaggcctgct
     1441 gaaatccggc atgaaactca ttgcggaagc ccggcgtgag cttcccgatg actcccggcg
     1501 tacatgagag cagcggagag aggactggat cggcgcaccc ggtactggaa aggaccaggt
     1561 tttcgttaaa tggggtctcg agcgtgcgcg cataggagac gcgcagaact gtatttgtag
     1621 gcttgatgct ataggaaacg cccagacgcg gttctgcctg gcgttcgcga gtcaggccat
     1681 tgtacaaatc gccgcgcatt cccaggctga agagccagtt tcctgcggtg atctggtctt
     1741 cggcatacag ggcaatctct ttgatatcag cgcgtccatg aaagccatat aaaccgccgc
     1801 ttcgcgtcag gtcatagggc agaagatcag aattaaagtt tgcattcggc tggtcaccgg
     1861 cagcagcgca ctgtgagggg tctgtaaatc caatcactgg attaccgttt gcatccaggc
     1921 aaggcgaatt cagcgtagga ttgactatgc caagcgcatc attttcgcgc aggaaagtat
     1981 gttcgtatac cgccccggcc ttgaagttat gcgctcccct tacataggag atgtctgtat
     2041 gcgctccggt attgagaagg gtacgacgct gctggaccgt ttctgactgc gtaggcgaaa
     2101 agtctgcaaa aggatttcta ctgggatagt agttgtaggc gtccttgcgc gcatacgcac
     2161 caaagttgaa gacagagtag ttattgatga gccgcgtata ggtgggagcg atgttgaagg
     2221 tctcaatctt cgatttctgg tcggtctcac cgacgctgtt gccaaactgg tccagaatat
     2281 tgaggttgtc gtatgcattc ggtgtctgga accaggaacg cgtatagttc aggttgagat
     2341 gaatggaatc ggcctgcgta aagttgtaat ccacgcggtc aaagatgttt tgctcattgc
     2401 cgcgtgcgtg aaagaccttg aattccggcg gatccagaaa gcggctggtg ttcagaccat
     2461 tagcggtaat gaagttgccc cagttgtccc cgccatagcc cagatcaaaa ccgccattga
     2521 ctgaaccaaa tgtgccatag gaagtgttga tgctgcctgt cggccttttc agtccctgtc
     2581 cggaacgtgt cgtgaccttg atgaccaggc ttgttttgtc gccatactcg gctggcggcg
     2641 caccgtcaat cacctccagg gactgcaccg aatctgcggg aatctggttg gaaaaaacct
     2701 tgctctgctg gtcgctgatg ggctgtccgt ctaccgaaaa ggagttggaa gcatggtcac
     2761 ctaatccatg aaagaggcca ttggagtcgg ctgccacacc tggcgacgcg agcgtaacca
     2821 gagagcttac ggaagaagac tggctctcaa gaggcagctt ctgcatcagg ccgcggtcaa
     2881 tgtctgtgtg catggtggtg tcattttcga ccaggtcttc ccctgtaaca accaccgtcg
     2941 tattcgaagc ttccccgagc ttcatgctga catttgcagt tacaggaacg actgagttca
     3001 cttccacatc ctgcgtggca ttactgaagc cggtcatcgt aactgtgagg tggtacggat
     3061 tgaagggtac gttggggaac tggaaatggc ccattttgtc cgttgtgacc gtgcgctcat
     3121 atttgctcac cgggttgacg atcgtaacgg cggcccctgg cacaactgct ccagtcgggt
     3181 cagtgactgt gcctgtaatc gtgccggaat tgctggtaga ttgtgcggac aacaaacatg
     3241 aagagag
//
LOCUS       DASRBV010000044         8303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_407284, whole genome shotgun sequence.
ACCESSION   DASRBV010000044 DASRBV010000000
VERSION     DASRBV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407284"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VFA02_01945"
     CDS             <1..1598
                     /locus_tag="VFA02_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU08638.1"
                     /translation="QDKSLYFKLPPLMTGSAPTAASDTSPAPFKTLEVAELADTGLAP
                     DYSKYLLTGATGLPQHVVDTRLKNVYDLREGVYQLTPSIKYDDYASSPVHRFFQMWQQ
                     ADCNVYYATEDNPSGCLNDLFPWVEVSVGAGSNGKAQPQGFNDLSTGEGSTAMGFYNV
                     QQGDAPYLKKLADEYTLSDNYHQAAMGGTGANHIFLGYGDALFYSDGKGHATTPPSNQ
                     IEDPDPQSGTNNFYKQDGYSGGSYVNCADPNQPGVGVIRDYLQSLDRPVKPNCQPGHY
                     YLVNNYNPGYLGDGTVDHTTPYAIPPSSVRNVGDALLAKNVSFRYYGEGWDLYVTDPT
                     YSSPYNQYCNICNIFQYSTRFMTDPALREEHIADTPRLYEDLATGELPAISYVKPSGF
                     NDGHPASSKLDLFEGFVKDVLERLQANPKLWASTAVFVTFDEGGGYYDSGYIQPLDFF
                     GDGTRIPLIIVSPYTRGGRISHDYADHVSILKFIEKNWKLSPLTGRSRDNLPNPIVTK
                     DNPYVPTNSPAISDLMDAFHFSK"
     gene            1747..3162
                     /locus_tag="VFA02_01950"
     CDS             1747..3162
                     /locus_tag="VFA02_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU08639.1"
                     /translation="MSVNRRDFLRSFATVSGAALTSTVAHSQPQTRPMDDILPNPDRS
                     GIEHIVVVMMENRSFDHLLGWMPNANGRQAGLTYLDNSGVAHPTYRLNYYVGCQHPDP
                     DHSYAGGRSEYNGGKMDGWLRTSTNDLFSIGYYYEEDLPLFSTLARNYTTLCNYFPSI
                     LSSTFPNRIFQHAAQTDRLDNSLHLSALPTIWDRLMGAGVSCRYYYSNVPFLALWGLD
                     YIGISALFEDFLEDAANGTLPAVSFVDPWFTILDDGTGNDDHPHADLRKGEIFLRQVV
                     EALSHGPGWANTVLVINRDEWGGFFDHVVPPRVIAPNSVDTDLVDGKALLGCRVPTLI
                     VSPFAKGNPAKPRVNSLLYDHTSVLKLIEWRWQLQPLTARDASNETGNLAYALNFQSQ
                     DTSLPNLPVIAEPPLDPCTLDDVLEGLDAQNKTVTVDEQKATQIATQFHNRGVDNETY
                     DFYLLLKSERTAGWRLPANLK"
     gene            complement(3324..3896)
                     /locus_tag="VFA02_01955"
     CDS             complement(3324..3896)
                     /locus_tag="VFA02_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZU08640.1"
                     /translation="MRQIKTCLGVAMISLALLCGARAEEQGPLMQPPPMERAMGGRML
                     HGRWWDNPKIAQQIGLTADQQKKMDDIFQQNRLKLIDLSAALEKQEVIMQPLIESDQP
                     DEGRILAQIDAIAQARAELEKNNARMLLEIRKVLTPDQWKKVKAIQPEEKRIVIHRFG
                     PGEDRRRDDAPPLPPLPDGGEGKQLQPPPQ"
     gene            complement(3883..4341)
                     /locus_tag="VFA02_01960"
     CDS             complement(3883..4341)
                     /locus_tag="VFA02_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08641.1"
                     /translation="MRNKTTIRQTEDDAATRRDLEAMAHAIGRFRSAVWHIAEQESSQ
                     PMQVQFEQAARRRRSVQRRVMLEWAFAGFAGVAALIPAAGYYRHHQEQARIEQQQQAL
                     KQREADAQLLDQIADEVSEAVPDAMRPLAEMDAMYGQNSTGQQGKTNATN"
     gene            complement(4338..4928)
                     /locus_tag="VFA02_01965"
     CDS             complement(4338..4928)
                     /locus_tag="VFA02_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08642.1"
                     /translation="MEQTAVTEMAPVAQADAQEHALLNLADFDEVVRLYRPRIFRFLL
                     ASLRDRDTAENLTQDCFLRAYMARERFRGDCSLSTWLMKIAMNLLRDHLRSRRMRFWT
                     RTRESALDPMDVSDWLPDERSSPEESFAARVQVKAVWDAVNKLPANQRSVFVLRFVEE
                     MELKEIAEVTGMNPSTVKTHLYRALAAVRARAGGGA"
     gene            complement(5038..6459)
                     /locus_tag="VFA02_01970"
     CDS             complement(5038..6459)
                     /locus_tag="VFA02_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZU08643.1"
                     /translation="MDLSIRSFALALVIAALSTVSDGATRDLDATGSPVRSAPVDPAI
                     AHVLQQISPQQIRHTIETLAGFHTRNTLSSMDTDLPKGQGINAAADWIESEFRRYSDA
                     CGGCLEVRRDTFTESPQARIPRPTTITNVYAILRGADPVQAKRMYLVTGHYDSRNTDV
                     LDDHGFAPGANDDASGVAVSLECARVLSKLKPPATLVFVAVAGEEQGLNGSRHLAKLA
                     KSEGWQLEGVLNNDIVGGNTTPGDQFQDKTAVRIFSEGIPSTATPEQTRLLESIGYES
                     DSPSRELARFIYDTGQTYHGIGRYFHPVLEFRRDRFLRSGDHVSFNQEGFPAVRFTEW
                     REDFNHQHQNVRTENGVEYGDLPKFVDYQYVASVAALNAATLASLAYAPPAPEDVRIV
                     TRNLDNNSTIEWKEGASASSGTKYEIVWRELTAPVWQFSLPVKAGEHSVTIPVSKDNV
                     IFGVRAVDALGHRSPAVAPIPER"
     gene            6599..6793
                     /locus_tag="VFA02_01975"
     CDS             6599..6793
                     /locus_tag="VFA02_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08644.1"
                     /translation="MPTCPECESVLDFEEEEVEEGDVVVCDECGTEFEVVATDPLELA
                     KVEEDYEDEDEDFSDEEEEE"
     gene            6790..7143
                     /locus_tag="VFA02_01980"
     CDS             6790..7143
                     /locus_tag="VFA02_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU08645.1"
                     /translation="MKQFCRTHFEKAGTVLALALCFLLAYGALAQDTRSVEGKVYAQG
                     SPVAGAVVYLQDSKTNNVKSFISTQDGSYRFGQLSTDVDYQIWAEYKGSKSEKKNISS
                     FNSKKQLVIDLHIKG"
     gene            complement(7311..>8303)
                     /locus_tag="VFA02_01985"
     CDS             complement(7311..>8303)
                     /locus_tag="VFA02_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08646.1"
                     /translation="SINNFQSDYLTRDDERAVREQVPSVMYSSPMLEMHDRISFGQGR
                     VKDVLVLGVSPEYRMVRNLKILSGRFFDDQDEETHTKVAVVTQDFAIAMFGSVESAVN
                     QSFQISGIPFTIIGTFRESVDTFGQSEIADQTILIPYSVARYFTGTDNVKQIFFSIRD
                     QSDVEGAAQEIVRVVSARHQKNSVYKANTLTALLTTAAKIANGLTVVLLLITTVTLAV
                     GGVGIMNIMLATVRSRIREIGIRKALGATSREIKLQFLIEAVFISLAGGIVGIVLGLA
                     LPFSVRFFTDYRIPISGWSVIIALLTSTVVGVVFGTLPANRAAQLDPVESLKYE"
BASE COUNT         1784 a         2457 c         2245 g         1817 t
ORIGIN      
        1 cacaggacaa gagtctttat ttcaagcttc ctcctctgat gaccggcagc gcgcccactg
       61 cggccagcga tacttcccca gcgccattta agaccctgga agttgcagag ctggccgaca
      121 ccggactcgc tccggactac agcaaatatc tgctcaccgg ggcaacgggc ctgccgcagc
      181 acgttgtgga tacccgtctt aaaaatgtgt atgacctgcg cgaaggcgtc tatcagctca
      241 cgccctccat taagtatgac gattacgctt ccagccccgt gcatcgcttt ttccagatgt
      301 ggcagcaggc ggactgcaac gtctattacg caaccgagga caatcccagc ggttgcctga
      361 acgatctgtt cccgtgggtg gaagtcagcg ttggcgcagg cagcaacgga aaagcgcagc
      421 ctcaaggatt caatgatctc tcgacgggcg aaggctccac agcgatgggc ttttacaacg
      481 tccagcaggg agacgcgcct tatctgaaga agctggccga cgaatacaca ctgagcgaca
      541 actaccacca ggcagccatg ggcggcactg gcgcaaatca catcttcctg ggttatggtg
      601 acgctctctt ttacagcgat ggcaaagggc acgccacaac tccgccgtcg aaccagatcg
      661 aagaccctga tccgcagagc gggaccaaca acttttacaa gcaggacggc tactccggcg
      721 gaagctatgt caactgcgcc gaccccaatc aacccggcgt tggcgtgatc cgcgactacc
      781 tccagtctct ggaccgccct gtaaaaccga actgccagcc aggccactac tatctggtga
      841 acaattacaa ccccggctat ctgggcgatg gaactgtgga ccacacaacg ccctacgcca
      901 ttcctccaag ctctgtgcgc aatgtgggcg atgctctgct tgccaagaac gtctccttcc
      961 gttactacgg cgagggctgg gacctttatg tcacggaccc aacctactcc agtccttata
     1021 accagtactg caacatctgc aatatcttcc agtactccac gcgttttatg acggaccctg
     1081 cactgcgtga agaacacatt gcagacacgc cgcgtctcta tgaagacctg gcgacgggcg
     1141 agcttcccgc tatctcttac gtaaagccaa gcggatttaa tgatggccac ccggcatcat
     1201 cgaagcttga tttgttcgag ggctttgtga aagacgtcct tgagcgtttg caggcaaacc
     1261 cgaagctctg ggcctcgacg gccgtcttcg tgacctttga cgaaggtggc ggttattacg
     1321 actccggata catccagccg ctggacttct tcggagacgg cacgcgcatt cccctcatca
     1381 tcgtcagccc ttatacgcgc ggagggcgca tctcgcacga ttacgctgac cacgtttcca
     1441 tcctgaagtt cattgagaaa aactggaaac tttcgcctct cactggccgc agccgcgaca
     1501 acctgcctaa ccccatcgtc acaaaagaca acccttacgt ccccaccaac agccccgcca
     1561 tcagcgacct gatggacgcc ttccacttct cgaaataacc gctgctgctt tcccggattc
     1621 cactccattc tgtggaatcc gggatttttt ttacattgcg ttcgatgcgt gttcatgcgc
     1681 cttttacctg tgacggcata taaagcaagc gcacgccatt ttctccttcc ggagggtccc
     1741 cccccgatga gtgtgaaccg cagagatttt ttgcgcagct tcgccactgt ttccggagca
     1801 gcactgacaa gcaccgtagc acacagccag ccgcaaaccc gccccatgga tgacattctg
     1861 cccaatccgg accgttccgg catcgaacac atcgtagtgg tcatgatgga gaaccgcagc
     1921 tttgatcatt tgctgggatg gatgccgaat gccaacggac ggcaggctgg gcttacctat
     1981 ctggacaaca gcggtgttgc gcacccaacg taccgcctga actactacgt cggatgtcaa
     2041 catcctgacc ctgaccactc ttatgccgga ggccgctctg aatacaacgg cggcaaaatg
     2101 gacggatggt tgcgcaccag caccaatgac ctcttcagca tcggctacta ttacgaagaa
     2161 gacctgccgc tcttcagcac attggcgcgt aactacacca cgctttgcaa ctactttccc
     2221 tctattcttt cttccacgtt tcccaatcgc atctttcaac atgccgcgca gactgaccgg
     2281 cttgacaact ctttgcatct ttccgctctg cctacaatct gggacaggct gatgggcgca
     2341 ggtgtgagtt gcagatatta ctactcgaac gtgccttttc tggcgttgtg gggactggac
     2401 tacatcggta tctctgcgct gtttgaggat tttctggaag atgcagccaa cggaacactg
     2461 cccgccgttt cctttgtaga cccgtggttc acgattctcg atgatggcac cgggaatgat
     2521 gatcatccac acgccgacct gcgcaaagga gagatctttc tgcgccaggt cgtagaggcg
     2581 ctgtcccatg gccccggctg ggccaacacc gtgctcgtta tcaatcgcga tgagtggggc
     2641 gggttctttg accatgttgt gcccccgcga gtaattgcgc cgaacagcgt agacactgat
     2701 cttgtggacg gaaaagcgct gcttggatgc cgtgttccca cgctgattgt ctcgccattt
     2761 gccaaaggca atcccgcaaa gccgcgtgtc aactcgctgc tgtatgatca cacatccgta
     2821 ttgaagctga tcgaatggcg gtggcaattg cagccactca ccgcccgcga tgcgtcgaat
     2881 gaaaccggta accttgccta tgcgctcaat ttccagagcc aggacacgtc cctgcccaat
     2941 ctgcccgtca ttgctgaacc accacttgac ccctgcacgc tcgacgatgt cctggaagga
     3001 ctcgacgcgc agaacaagac ggtaacagta gacgaacaga aggccacaca gattgccacg
     3061 cagttccaca accggggagt ggacaacgaa acttacgatt tttatctgct gttgaaatcc
     3121 gaaaggacgg ccggctggcg tctgccggca aacttaaaat gagacctcac agcatttctg
     3181 gttttactgt catccgcaaa aaggccgccc atcattctgg gcgtggaaaa tcttagcagt
     3241 attggcgaaa ccctcagccg gtttgcggaa cacttccagg tcaacagcag gaaagagcgg
     3301 cctccgaagg ccgctcctgt tttttactgt ggtggtggtt ggagttgctt gccttctccg
     3361 ccgtccggta gaggtggcag aggcggcgca tcgtccctgc gcctgtcctc tcccgggccg
     3421 aagcggtgaa tcacaatgcg cttctcttcc ggctgaatgg ccttgacctt cttccactgg
     3481 tcgggtgtaa gcactttgcg gatttccagc aacatgcgcg cattgttctt ttcaagctcg
     3541 gcgcgcgcct gggcgatggc atcaatctgc gccagaatcc tgccctcgtc cggctggtcg
     3601 gattcaatca gcggctgcat gatgacttcc tgtttttcca gcgccgcact caggtcaatc
     3661 aacttcagcc ggttctgctg gaagatgtcg tccatctttt tctgctggtc ggcggtcaat
     3721 ccaatctgct gcgcaatctt gggattgtcc caccagcgtc catgcagcat tcgtccaccc
     3781 atggccctct ccatcggagg aggctgcatc agcggtcctt gttcctcggc ccgcgctccg
     3841 cataaaaggg ccagcgaaat catcgccacc ccaaggcatg ttttaatttg tcgcattcgt
     3901 tttcccctgt tgtcctgttg aattctgccc atacatcgca tccatctctg ccagtggacg
     3961 catcgcatcc ggcactgctt ctgaaacttc atcagcgatc tggtccagca actgcgcatc
     4021 ggcttcgcgc tgcttcaatg cctgctgttg ctgctcgatg cgcgcctgct cctgatggtg
     4081 ccggtagtac ccagccgccg gaatgagcgc agccacaccg gcaaagcccg caaaggccca
     4141 ctccagcatg acgcgcctct gcaccgagcg cctgcgcctg gcggcctgct caaactggac
     4201 ctgcattggc tgggagcttt cctgttccgc aatgtgccac acggctgaac ggaaccggcc
     4261 aatggcatgg gccatcgctt ccagatcgcg ccttgttgcc gcgtcgtcct ctgtctgccg
     4321 gattgttgtt ttgtttctca tgctccgcct cctgcccgtg ctcttactgc cgccagagcg
     4381 cggtaaagat gtgttttcac cgtgctcgga ttcatccctg tgacctccgc aatctctttg
     4441 agttccattt cctccacaaa ccggagaaca aacacgctgc gttggtttgc cggtagcttg
     4501 ttgactgcgt cccagacggc ctttacctgc actcgcgccg cgaagctttc ttccggagaa
     4561 ctcctctcat cgggcaacca gtcgctcacg tccatcggat cgagtgccga ctcgcgcgtt
     4621 cgggtccaga aacgcattct ccggctgcgc agatggtccc gcagcaggtt catggcgatc
     4681 ttcatcagcc aggtgctcag gctgcaatca ccgcgaaagc gctcccgtgc catataagcg
     4741 cgcagaaagc agtcctgcgt caggttttcc gccgtatcgc ggtcgcgcag cgaagccagc
     4801 agaaacctga agatgcgcgg acgatagagc cgcaccacct cgtcgaagtc cgccagattc
     4861 agcagcgcgt gttcctgcgc atccgcctgg gccaccgggg ccatctcagt cacagcggtc
     4921 tgttccatgc aaaggaatag acgggagggg tagaggcgga gtttacgtgg ccatgcgtaa
     4981 atagcagcgg tgcaggagca ggggtttgcc gcccaaagcc cgctcaatac ctggcttcta
     5041 ccgttccgga atcggggcca cggcggggct gcggtgccca agggcgtcta cggcgcgcac
     5101 gccgaaaatg acgttgtctt tggaaaccgg aatggtcacg ctgtgctcgc ccgccttgac
     5161 cggaagcgaa aattgccaga ccggcgcggt caattcgcgc cagacgatct cgtatttcgt
     5221 tcctgacgaa gcgcttgcgc cttccttcca ttcaatggtt gagttgttgt cgagattcct
     5281 ggtcacgatg cggacgtctt ccggggctgg aggcgcatag gccagcgatg ccagcgtggc
     5341 ggcattcagc gcggcgacgc tggccacata ctggtaatcc acgaatttgg gcaggtcgcc
     5401 atattcgaca ccgttttctg tccttacgtt ctggtgctgg tggttgaagt cctcgcgcca
     5461 ctcggtaaag cgcacggcgg ggaagccttc ctgattgaaa gagacgtggt ccccactgcg
     5521 cagaaaacgg tcccggcgga actcaagcac cggatggaaa taccggccaa tgccgtgata
     5581 ggtctgaccg gtatcgtaaa tgaaccgcgc cagctcacgg gagggagaat cgctctcgta
     5641 cccgatagac tcgaggagcc tggtttgctc aggcgtggcg gtactgggta tgccctcgga
     5701 aaagatgcgc acggcggtct tgtcctgaaa ctggtcaccg ggagtggtat ttccgcccac
     5761 aatgtcgttg ttcaggacac cttccaattg ccagccttcc gatttggcca gctttgccag
     5821 atggcggctt ccgttcagtc cctgctcttc tccggccacc gcgacaaaga ccagggtcgc
     5881 aggaggcttc agcttcgaga gcacgcgggc gcattccagg cttacggcca cgccggatgc
     5941 atcgtcattg gccccgggag caaagccgtg gtcatccaga acatcggtat tgcgcgaatc
     6001 gtaatgtcct gtcaccagat acatccgttt tgcctggaca gggtcggccc cgcgcaggat
     6061 ggcgtagaca ttggtaatcg tggtcggcct ggggatgcgg gcctgtggac tttccgtaaa
     6121 cgtgtcccgt ctgacctcaa ggcagccgcc gcaggcatcc gagtagcgcc tgaattcaga
     6181 ctcgatccag tcagcggcgg cgttgatgcc ctgccctttg ggcagatcgg tgtccatgct
     6241 ggaaagcgtg ttgcgcgtat ggaagccggc cagcgtctct atggtgtgcc ggatctgctg
     6301 tggagaaatc tgctgcaaaa cgtgtgcaat ggctgggtcc acgggcgccg aacggacagg
     6361 ggaacctgtg gcatccagat cacgggtagc accgtctgag actgtagaca gggccgctat
     6421 cacaagcgca agggcaaacg atcgaatgct gagatccata atttcaatcc gaggtggaaa
     6481 taaattcgta agcaactctt gacgcaatcg cggattctct ttaaaaacta acgaagttgc
     6541 atcctgtaaa ttgttgtcat catcctaata ctccgactta tcgaagaggg aggaatttat
     6601 gcctacttgc cctgaatgtg agagcgtgct cgattttgaa gaagaagaag tggaagaagg
     6661 cgacgtggtc gtctgtgatg aatgcggcac tgagtttgag gtagtcgcca ccgatcctct
     6721 ggagcttgca aaagtagaag aagactatga ggatgaagat gaggacttct cagacgaaga
     6781 ggaagaggaa tgaagcagtt ttgccgtaca cattttgaaa aggccggcac ggtactggcg
     6841 ctggccttgt gctttctgct tgcttatggc gccttggcac aggacacgcg ctccgtcgag
     6901 ggcaaggtct atgcgcaagg cagtccggtg gccggggccg ttgtctatct gcaggacagc
     6961 aaaacgaaca acgtgaagag cttcatttcc acccaggacg gcagctaccg cttcggccag
     7021 ctttccaccg atgtggatta ccagatctgg gccgaatata aaggcagcaa gagcgagaag
     7081 aagaacatca gctcgttcaa ttccaaaaaa caactcgtga ttgacctgca catcaagggt
     7141 taaggcgttt tcacagactc tgtacacttc atttgcactg cgcggggtca ccggctccct
     7201 gctggccgca acgtctgtag tttccgggca tggttcagac gatctgtgac ggtaagtcat
     7261 agatttttca tcctgtccct gaacgcagtg aagcgaaggc ccctgctttt ctactcgtac
     7321 ttcagtgact ctaccggatc gagctgcgca gcgcggtttg caggcagcgt gccgaagacc
     7381 actccgacca cggtcgaggt gagcagcgcg atgatgaccg accatccgga aataggaata
     7441 cggtagtcgg taaagaagcg gacagagaac ggaagcgcaa gccccagcac aatgccgaca
     7501 atcccgcccg caagggagat aaagacagct tcaatcagaa actggagctt gatctcgcgc
     7561 gacgtagcac cgagtgcctt tcgtatacca atctcacgaa tacgcgagcg caccgtggcc
     7621 agcatgatat tcataatgcc gacgccgccc acggccagcg tcacggtggt gatcagcaga
     7681 agcacgactg tcaggccatt ggcgattttg gccgctgtcg taagcagcgc ggtgagcgta
     7741 ttggctttat agaccgagtt cttctggtgc cgcgcgctta cgacgcgcac aatctcctgc
     7801 gcagcgcctt ccacatcgct ctggtcgcga atggagaaga agatctgctt cacattgtct
     7861 gtacccgtga aataacgcgc aacagaatat gggatgagaa tggtctggtc ggcaatttcc
     7921 gactggccga atgtgtctac gctttccctg aaggtcccga tgatggtgaa cgggataccg
     7981 ctgatctgga agctctgatt aacggccgat tccacgctgc caaacatggc tatggcgaag
     8041 tcctgggtca cgacggcaac cttggtgtgg gtctcctcat cctggtcatc aaaaaaacgg
     8101 ccggagagga tcttcagatt gcgcaccatg cggtactcgg gacttacacc aagcaccagg
     8161 acatctttca cccggccctg cccaaagctg atgcggtcat gcatctccag catgggcgaa
     8221 gaatacatga cggacggcac ctgttctctt acggcgcgct catcatcacg cgtgagatag
     8281 tcgctttgga aattattgat aga
//
LOCUS       DASRBV010000045        10894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_407326, whole genome shotgun sequence.
ACCESSION   DASRBV010000045 DASRBV010000000
VERSION     DASRBV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407326"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1759
                     /locus_tag="VFA02_01990"
     CDS             71..1759
                     /locus_tag="VFA02_01990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU08647.1"
                     /translation="MARIVKAPLLFAAVFGVVLLAHLPLLHLPYYWDEAGYYIPAAYD
                     FFRTGSLIPFSTLSNAHPPLPPVYLALWWKVFGFAPWVTRTAVCAIASLGLLGVYRIG
                     LVSTRRWQVAVAVAILTAMYPIWFAQSSLAHADIFAAAATLWALAFFLEDRTWASVLC
                     FSLAALSKETAIVTPLALVAGGIYQRLASRSPGLLQSKKTGHSGPSPQTNLGAFLLPV
                     LPLACWYAWHWHRTGSIFGNPEYLRYNATATMNPVRILLAFAHRCLQITGHMNLFVPV
                     LITLACMMLPPVEEGLPSPAESTPLPAPLRNPCEQRRRISARIQAVFYVVIFVNLVLF
                     SVLGGALLTRYLLPLYPLVLLLCVNTFYRRVRRWPALVALSAAGFVAGIFVNPPYRFA
                     PEDNLAYRDVVLLHQAAIRQIEQHYPGATVLTAWPGTDELHKPELGYVSQPIQTVAID
                     NFSFNQMDAAAQLGTQYSVALVFSTKYDPPHLWLGLGRRNEALDVRYFDFHRDLPPEE
                     IARLLGGTIVWRGERQGEWAAVLHFDRAEEAWVKPGTSQHADRFTADLFQYNQP"
     gene            1770..3389
                     /locus_tag="VFA02_01995"
     CDS             1770..3389
                     /locus_tag="VFA02_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08648.1"
                     /translation="MNSFRSLVCVLLLMAAPFLAAQQSATTVSRPQSEAAPAAHGRLL
                     LVLPFENHSGHADLNWIGEAFPEIFNRRLSAAGFLPISRNDRMYALDRLGLPPNFQPS
                     RASTIRIAQTLDADYVVFGSFTTSGNSLNATAQVLDVTGLRLGNPINMQADMTRLLDI
                     VNTLAWYTARQLDPKYAVAQQTFLNADSDLRVEAFENYIRGLTEDSIGERLRHLREAV
                     RLAPHFSPAMLALGKAYFYNQNFDLAAATLGNLPKDDPNALEADFYRGLAFFYTGKYL
                     EAEDAFAFDTTRLPLPEVVNNQGVAAARRGRDGTSLFQQASAADPKDPDYHFNLAVSL
                     RRRGDIPGAVREIDQAVKLRPQDTDAVYFANSLHSEQQNPAPAAQKADLPLERIKRSY
                     NEASFRQAAFEMEQMQNMRLAMLPPAEHAEALVKDGTQFLNRGLLLEAEREFQAAIQA
                     DPNSASAHARLAEVRERTNDLDAARQEAQKSLQIKPNVAAHLVLARMDEQAHQLAPAA
                     NEISEALKIEPNNPNARVMKMALEQKGQQVP"
     gene            3437..4777
                     /locus_tag="VFA02_02000"
     CDS             3437..4777
                     /locus_tag="VFA02_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZU08649.1"
                     /translation="MPHTRPTDRLVWTTRHSLIVIFFLLFSACSLKAQPAPQIVLLRL
                     HDTIQPISEGYISRGLSHAGQLHASAVLIEMDTPGGMLESTRAIVHDILASPVPVIVY
                     VAPSGSRAGSAGFFILEAADIAAMAPGTNAGAAHPVVMGGNLSPEMKQKLENDTTAFL
                     RSYVSRRGRNTDAAQDAVLNSKSYTEREAEQLKLIDVVAPGDTALLDSLDGRTVTRFD
                     GTKTVLHTRNAHLVPVDTTIREEILDRMMDPNLAVLVLVIGALLIYLEFNTPGTIIPG
                     ALGTLLLLLALFSLNLLPVRYTSLLLIVAAFVLMLLEAKFGGHGVLAASGIICLVLGL
                     LTLVAGPVPEMRVSIGTAVGTGIAFGAITTFLVRLAWRARRNKSLTGPEALIGSIAIA
                     QQQLDPRGQVMVHGELWFAESPEPVLAGERVRVRDVRGLTLLVERVPDGKSTAK"
     gene            4829..5599
                     /locus_tag="VFA02_02005"
     CDS             4829..5599
                     /locus_tag="VFA02_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZU08650.1"
                     /translation="MGIPGLVVIAVIVLYLLNSIKILREYERGVIFRLGRALPQPKGP
                     GIILVFRPFDQIIRVSLRQEVLEVPPQDVITRDNVTIKVNAVITLRVLDPLKAVIEVS
                     NYVYQTSQFAQTTLRSVLGEVELDSLLAHRDALNQRIQTIMDQRTEPWGVKVVSVEVK
                     QVDLPEQMLRAMAKQAEAEREKRSKIINAEGEYAAAQRLVDAANLLATQPITLQLRYL
                     QTLTDIGTEKNTTVIFPLPVELLSLVQKFVPAKPEEQK"
     gene            5596..6264
                     /locus_tag="VFA02_02010"
     CDS             5596..6264
                     /locus_tag="VFA02_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU08651.1"
                     /translation="MRNVVEYEEENENESEITLNAGTLLGIFFGLVLVCGVFFGFGYS
                     MGRRGTTASANTTDAAPSTNTQAETPAPQPSGAPKPSAIQALQTDNSSPDVDTPAATQ
                     TVEGQSAPEEKPVPVLAKKPAPVPAVEQAASTVKPVLKPAALPVTPVTGTFMVQIAAV
                     SHQEDANTLANALRQHGYTVSVRNDPPDKLYHVQVGPFASRDAARAMRSRLLADGYNA
                     ILKP"
     gene            complement(6407..6616)
                     /locus_tag="VFA02_02015"
     CDS             complement(6407..6616)
                     /locus_tag="VFA02_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08652.1"
                     /translation="MATATLIIGLIFLGIGVLGHHFFTREKRQLHPDEHRARKWVVTI
                     GSLVAGLWLVAFSAAHLLHVYHPSF"
     gene            complement(6695..7444)
                     /locus_tag="VFA02_02020"
     CDS             complement(6695..7444)
                     /locus_tag="VFA02_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZU08653.1"
                     /translation="MRLKFAYILLFLPVAAYSQQVPSADQQRILDLTNQARAQQGLGP
                     LQWDPALAAAAQVHTQRMADQRTLTHQLPGEPDLAVRAGQQGAHFRAIAENIAMAGSL
                     NGLQNEWMKSPPHCANILDPRMDHIGVGLIQRGGYWYAAVDFDRAVASLSSSQIEQQV
                     AALLRQRGISQFRAPEAARKDCSLEEGDVSGTRPRFIMRWEGTSLDRLPVALEQRLRS
                     GRYTAASVGACSSQSAGNQGFTTYSIAVLLY"
     gene            complement(7523..7885)
                     /locus_tag="VFA02_02025"
     CDS             complement(7523..7885)
                     /locus_tag="VFA02_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZU08654.1"
                     /translation="MTEIIQNPKIVRAAGFAYRMRRRIATAAAIALAVFLGYHVIFGQ
                     NGITAYEQKRLEDKDLQKQIQQLQDENSRMKDHVDHLQNDPDAIEHEARARLHYTRPG
                     EVIYTLNDKPAADASAKK"
     gene            complement(7956..9353)
                     /locus_tag="VFA02_02030"
     CDS             complement(7956..9353)
                     /locus_tag="VFA02_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZU08655.1"
                     /translation="MATQIKFGTSGWRAIVAEEFTIANVRRAVSGIAKYVASQPGAKT
                     VLIGRDPRFMGEIFVAEAAKVLAANGVAPIVIPEAAPTPAIAYAVRTRKTGGSINFTA
                     SHNPPEYNGIKYSTPDGAPALPEVTSQIEAFIADLGFVAAPPGQKQKFEETDVRPDYL
                     KRLAELVDLKAIQKAGIKIVCDPFWGAGRGYASQLLAENGVSVATVHDYRDVLFGGHA
                     PEPDDHLLADCKAKMKETGAAIGIATDGDADRFGVVDGDGAFIQPNYIIALLFDYLVE
                     TRGWKNGVAKSVATTNLINALADHHHVQLYETPVGFKYIGALIDQDKIAIGGEESAGL
                     TIRGHVPEKDGIIAGLLIAEMVAVRGRSIGEQLKELFAKVGSFYPLRENFRLTPEVKE
                     KFTEKVKRDPHELGGRKVMQVVRTDGLKLILEDGSWVCYRLSGTEPVVRVYSEARKES
                     DFVPLSKAAKDWVLE"
     gene            9419..9736
                     /gene="cutA"
                     /locus_tag="VFA02_02035"
     CDS             9419..9736
                     /gene="cutA"
                     /locus_tag="VFA02_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZU08656.1"
                     /translation="MEQARIILSTTSSREEAQCIAHSLVDERLAACVNIMDGISSVYR
                     WQGAVEEAPEILLIIKSSVEKLEALEAALHRLHSYEVPEFLVLPVEGGSRAYLEWIRE
                     SVK"
     gene            9910..10056
                     /locus_tag="VFA02_02040"
     CDS             9910..10056
                     /locus_tag="VFA02_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08657.1"
                     /translation="MRVLFAVFVLSIVAIIWTAVAVARHVRRNGSQGERISIKASEPD
                     HEQE"
     gene            10122..10667
                     /locus_tag="VFA02_02045"
     CDS             10122..10667
                     /locus_tag="VFA02_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZU08658.1"
                     /translation="MLRWGTAIVAGSALVAVCAHISVPLFFTPVPMTLQPFAVLLLGL
                     FMEPAAAFAALVLYLAEGAAGLPVFTPQGLGGIAQILGPTGGYLMSYPFVAALVSFLY
                     RRLPVRNFWTALISTASGNALILAAGATWLAVLTHQTLGHTATLAVMPFLAGDALKVC
                     LAAAIAAGWLRMRRIEKTVVR"
BASE COUNT         2193 a         3045 c         3134 g         2522 t
ORIGIN      
        1 gaaaagcagg ctttttcatt ttgctgtttg aaaatggcgt atttctgcgt gatttttacc
       61 gggtctgtct atggcaagga ttgtgaaagc tccgctcctg tttgctgcgg ttttcggcgt
      121 cgtactgctg gcgcatcttc ccttgctgca cctgccctat tactgggacg aggcggggta
      181 ctacattcct gctgcgtatg atttctttcg caccgggtcg ctgattccct tctctacgct
      241 gtcgaatgcc cacccgccgc tgccgccggt ttatctcgca ctctggtgga aggtctttgg
      301 gtttgctccc tgggtgacgc ggacggcggt ctgtgcgatc gcttcgctgg ggttgctggg
      361 ggtatacaga ataggacttg tcagcacacg caggtggcag gttgccgttg ccgtagccat
      421 tctgacagcg atgtacccaa tctggtttgc gcagagttca ctggcgcatg ccgacatctt
      481 tgctgctgcc gcaactcttt gggcgctggc cttttttctg gaagacagga cgtgggcaag
      541 tgttctgtgt ttctcgctcg ctgctctttc caaagagaca gcgattgtga ctccgctggc
      601 gctggttgcc ggggggattt atcagcgctt ggcatcccgc tctcccggcc ttttgcaaag
      661 caaaaagacg gggcactctg gtccttcacc tcaaacgaat ttgggtgcat tcctgctacc
      721 tgttttgccg ttggcctgct ggtatgcgtg gcattggcac cggactgggt ccatctttgg
      781 aaaccccgaa tatcttcgct ataacgcaac agcgacaatg aatccggtgc ggatactgct
      841 ggcgtttgcg catcgctgtc tgcaaataac cgggcacatg aatttgtttg tgcctgtgct
      901 gattacgctg gcgtgcatga tgcttccgcc ggttgaagag ggccttccct cgccggctga
      961 aagcaccccc cttccagcac ctctgcgaaa tccatgtgag cagcgcaggc gtatttctgc
     1021 ccggatccag gccgtttttt atgttgtgat ttttgttaat ctggttttgt tttccgtact
     1081 tggcggagcg ctgctgacac gctatcttct gccgctttat ccgctggtgc tgctgctttg
     1141 cgtcaacacg ttttatcgaa gggtgaggcg atggcccgca ctggtggccc tgagcgctgc
     1201 cggatttgtc gctggtatct ttgtcaatcc tccgtaccgg tttgcgccgg aagacaatct
     1261 agcctatcgc gatgtggttc ttctacacca ggccgccatc cggcagattg agcagcacta
     1321 ccctggagcc accgtgctta ccgcatggcc tggaaccgat gagctgcaca agcctgaact
     1381 gggctatgtc tcacagccca ttcagaccgt tgcgattgat aacttctctt tcaaccagat
     1441 ggatgctgcg gcgcaacttg ggacgcagta ttctgtcgcc cttgtatttt caaccaagta
     1501 tgatccgccg catctctggc tgggtctggg ccgcaggaat gaagcgttgg atgtgcgcta
     1561 ttttgacttc catcgcgatc tgccgcctga ggagattgcg cggctgctcg gtggaacgat
     1621 cgtgtggcgt ggagagcggc agggagagtg ggcagcggtc ctgcactttg accgggcgga
     1681 ggaggcgtgg gttaagccag gaaccagcca acacgctgac cgctttaccg ccgacttgtt
     1741 ccaatataat cagccttgaa gtgcggtcat tgaattcatt tcgctctctt gtttgcgtgc
     1801 tgctgttgat ggcggccccg tttcttgcgg cacaacagtc tgccacaacg gtttcgcgtc
     1861 ctcagtcaga ggcagcgcct gccgcacatg gacggcttct tctggtcctg ccgtttgaga
     1921 accacagcgg ccacgctgac ctgaactgga ttggcgaggc ctttccggaa atctttaacc
     1981 ggcgtctgag tgctgccggg tttctgccca tcagtcgcaa tgaccgcatg tatgcgctcg
     2041 accgcctggg gcttccgccc aacttccagc cttcgcgggc aagcaccatt cggattgcgc
     2101 agacgctgga tgcggattac gtggtcttcg gctcgttcac aacgtcggga aattcgctga
     2161 acgccacggc tcaggtgctg gatgtaaccg gattgaggct cggcaaccct attaatatgc
     2221 aggctgacat gactcgcctg cttgatattg tgaacacgct ggcatggtat acggcgcgtc
     2281 agttggaccc gaaatatgcc gtcgcgcagc agacgtttct gaatgctgac tctgacctgc
     2341 gtgttgaagc ctttgagaac tacattcgcg gacttactga agactcgatt ggcgagcgcc
     2401 ttcgccacct gcgcgaggct gtgcggctgg ccccgcattt ctctccggcg atgctggcgc
     2461 tgggcaaggc atatttctat aaccagaact ttgatcttgc ggcggcgact ttgggtaatc
     2521 tgcccaagga cgaccccaac gcgctcgaag ctgattttta tcgcgggctg gctttcttct
     2581 ataccggaaa atatctggaa gccgaagatg ccttcgcttt tgataccacg cgcctgcctc
     2641 tgcctgaagt ggtgaacaat cagggagtgg ctgcggcacg gcgggggcgc gatggcacat
     2701 cgctgtttca gcaggcgagt gctgctgacc cgaaagatcc ggattatcac ttcaatctgg
     2761 cagtttccct gcgacggcgc ggagacattc ccggagcagt gagagaaata gaccaggcgg
     2821 taaagctgcg tccgcaggac acggacgccg tctactttgc caatagcctg cacagcgaac
     2881 aacagaaccc agctcccgcg gcgcagaaag ccgatcttcc actcgagcgt atcaagcgca
     2941 gctataacga agcgagcttc cgtcaggctg cctttgagat ggagcagatg cagaacatgc
     3001 ggctggccat gcttccgcct gcagagcacg cagaggcatt ggtgaaagat ggtacgcagt
     3061 tcctgaaccg cggactgctg ctggaggcgg aacgcgagtt tcaggcagcc attcaggccg
     3121 atccgaattc tgcctcagcc catgccaggc tggcagaagt acgcgagcgc accaacgacc
     3181 ttgacgcggc tcggcaggaa gcgcagaaat ctctgcagat taagccgaat gttgcggcgc
     3241 atctggtgct ggcgagaatg gacgagcagg cgcaccagct tgctcccgcg gcaaatgaga
     3301 taagcgaggc gctgaagatt gagccgaaca atccgaatgc ccgcgtgatg aagatggcgc
     3361 ttgagcagaa aggtcagcaa gtgccatgag cgtgtggagc ggggactcct ctgagaagcc
     3421 ccggtactcg gggccgatgc ctcatacaag gccaacagac aggcttgtat ggaccacccg
     3481 gcactctctg attgtcatct ttttcctgct cttttctgca tgctcactta aagcgcaacc
     3541 agcacctcag attgtgcttc ttcgtctgca tgacacgatt cagcccatca gcgaagggta
     3601 tatcagccgg gggctgtccc atgctgggca actccatgcc agcgctgtcc tgattgagat
     3661 ggacacacct ggaggcatgc ttgagtccac acgggcgatt gtgcatgaca ttctggcctc
     3721 acctgttccg gtgattgtgt atgttgcgcc ttccggcagc cgcgccggtt ctgccggatt
     3781 cttcattctt gaagctgcgg acattgcagc catggccccg ggaacgaacg cgggtgcagc
     3841 acatccggtg gtgatgggcg gtaatctcag tccggagatg aaacagaagc tcgaaaacga
     3901 tacgactgcg tttctgcgct cctacgtttc gcgtcgcgga cgcaacacgg atgccgcgca
     3961 ggatgccgtc ctcaactcga agtcgtatac cgagcgcgaa gccgagcagt tgaagctgat
     4021 tgatgtggtc gcgccgggcg ataccgccct tctggattcg ctggatggcc gcacggtcac
     4081 gcgctttgat ggcacaaaaa cagtgctgca tacgcgcaat gcgcacttgg ttccggtaga
     4141 tacaaccatc cgcgaagaaa tactcgaccg catgatggac ccgaaccttg cagtgcttgt
     4201 gctggtcatt ggtgcgcttt tgatttatct ggaattcaac actccgggca ccatcatccc
     4261 cggcgcgctg gggacgcttc ttttgcttct ggcgcttttc tcgctgaacc tgctgccggt
     4321 acgctacacc tctctgcttc tgattgtggc cgcgtttgtg ctcatgctgc tggaggccaa
     4381 gtttggcggg catggcgtgc tggcggcctc cggaatcatc tgcctggtgc ttggactgct
     4441 gacgctggta gcggggcctg tgccggagat gcgcgtgagt atcggcacag cggtcgggac
     4501 cgggatcgca ttcggggcca ttacgacgtt tcttgtccga ttggcgtggc gcgcgcggcg
     4561 caacaaatct ctcaccgggc ctgaggcgct gattggctcg attgccattg cccagcagca
     4621 gctcgacccg cgcggacagg tgatggtcca tggcgaactg tggtttgccg aatcgccgga
     4681 accagtcctg gccggcgagc gggtccgggt gcgcgatgtg cgggggctta cgctgttggt
     4741 agagcgggtt ccggacggca aatctacagc aaaatagacg cttttctttc gtcgcgctgc
     4801 gatacaatcc cagtgcgaga ggggaactat gggcattccc ggcctggtcg tcattgcagt
     4861 catcgtcctt tatctgctga attctatcaa gattctgcgc gaatacgagc gcggggtcat
     4921 cttccggctt gggcgcgcgc tgccacagcc caaaggcccg ggaatcatcc ttgtcttccg
     4981 gccctttgac cagatcattc gcgtttcgct ccgccaggag gtgctggaag ttcctccgca
     5041 ggacgtgatt acgcgggaca atgtgaccat caaggtcaac gccgtcatta cactgcgcgt
     5101 gctggatccg ctcaaggcgg tcattgaagt ctcaaattat gtctaccaaa cctcgcagtt
     5161 tgcgcagacc acgctgcgct cggtgctggg cgaggtagag ctggacagtc ttctggcgca
     5221 tcgcgatgcc ctgaaccagc gcatccagac catcatggac cagcgcaccg agccttgggg
     5281 tgtgaaggtg gtcagcgttg aggtaaagca ggtggacctt cccgaacaga tgctgcgagc
     5341 gatggccaaa caggccgagg ccgagcgcga aaaacggtcg aagatcatca acgccgaggg
     5401 cgaatatgcc gccgcgcagc gtctggtgga tgctgccaac ctgctggcca cccagcccat
     5461 tacactgcaa ctgcgctatc tgcaaacgtt gaccgatatc ggcacggaaa agaacacaac
     5521 ggtcatcttt cccctgcccg tggagttgct ttcgctggtc cagaagtttg ttcctgcaaa
     5581 gcctgaggag cagaaatgag aaacgtagtc gagtacgagg aagaaaacga gaacgaatca
     5641 gagattactt tgaatgccgg cacgctgctg ggaattttct tcggcctggt gctggtgtgc
     5701 ggagtgtttt ttggctttgg ctactcgatg ggacggcgtg ggaccaccgc ttcagcgaac
     5761 actacagatg ccgccccctc cacaaatacg caggcggaga cccctgcgcc gcagccttcc
     5821 ggcgctccca aaccatcggc gatacaggcc ctgcaaacgg acaattcttc tccggacgtg
     5881 gacacccctg ctgcaacgca gactgtagag ggccagagtg ctccggagga gaagcccgta
     5941 cctgtactcg cgaagaaacc agctcctgtc ccggcagtag aacaggctgc atccacggtc
     6001 aaaccagtcc tgaagccggc ggctttgccg gtcacccctg ttacgggaac atttatggtg
     6061 cagattgccg ctgtgtccca tcaggaagac gcaaatacgc tggccaatgc tctgcgtcaa
     6121 catgggtata cggtgtctgt gcgcaatgat ccgccggaca agctctacca cgtacaggta
     6181 ggcccgttcg cgtcgcgcga tgcagcccgg gccatgcgct cccggctgct ggcagatgga
     6241 tacaacgcca tcctgaagcc ttaagaaaat ttgcttcgcc gcaaatgcat cactggttcg
     6301 ccttcgaggt cgttcgcgtc aaggtatgac gcacacaggc accacgggcg ttgtggcaat
     6361 ctacacggtt gctctggcca tgcagagagt caaggcagtg aaaggcttag aagctgggat
     6421 gatacacgtg cagcaaatgc gctgcggaaa aggccacgag ccacagaccc gcaaccagtg
     6481 agccgatggt gacgacccac ttgcgtgccc tgtgctcatc cggatgaagc tgccgttttt
     6541 cacgggtgaa gaaatggtga cctagaacgc caattccaag aaaaataagg ccgataataa
     6601 gggtagctgt tgccatggcg caacactcta ctcttatgag atgacggttg ggaagagaaa
     6661 ttttaccgtt tatttcgcta tactgcaaat tctttcaata cagtaaaacc gctatgctgt
     6721 acgtagtaaa cccttgattt ccagcacttt ggctggagca cgccccgaca gaagccgcag
     6781 tatatcttcc ggaccggagc cgctgctcca gggccacagg cagccggtcg aggcttgtac
     6841 cttcccatcg catgataaag cgcggcctgg tcccgctgac atcgccttct tctaaagagc
     6901 aatctttgcg cgccgcctct ggtgcgcgga actggctgat tccccgctga cggaggagcg
     6961 cggccacctg ctgctcgatc tggctggagc ttaaagaagc cactgccctg tcaaaatcca
     7021 ctgccgcata ccagtatcct ccgcgctgaa tgagtccgac tccaatgtgg tccatacgag
     7081 gatcgagaat attggcacag tgcggtggcg atttcatcca ttcgttctgc aaaccattca
     7141 ggctaccagc catcgcaata ttttcagcaa tcgcgcggaa atgtgctcct tgttgccctg
     7201 cacggacagc aaggtcgggc tcacccggaa gctggtgggt cagtgtacgc tgatctgcca
     7261 tgcgctgggt atggacctgg gccgcagccg cgagcgccgg gtcccattgc agcgggccta
     7321 gtccctgctg cgcccgtgcc tggttggtga ggtcgaggat gcgctgctgg tcagcagaag
     7381 gcacttgttg cgaatatgcg gctactggaa gaaacaagag aatgtaggcg aacttcaaac
     7441 gcatagaaat ttcgatgcgg gaaaaagaag tttcccgctc tctgaagaga aaaacacctt
     7501 cgacggtgct ggagttgtgg ttttattttt ttgcggaagc gtctgcggcg ggtttgtcat
     7561 tcagcgtata aatgacctcg cctgggcggg tgtagtgcag gcgcgcgcgg gcctcatgct
     7621 cgatcgcatc gggatcattc tgaagatggt ccacatggtc tttcatacgc gaattttcat
     7681 cctgcaactg ctggatctgc ttttgcaggt ctttgtcttc cagacgcttc tgttcatagg
     7741 cggtgatgcc gttctggcca aagatgacgt ggtaccccag aaacacggcc aaagcaatcg
     7801 ccgcagccgt ggcaatgcgc cgccgcatgc ggtaggcaaa gccggcggcg cggacaatct
     7861 tcgggttttg gataatctcg gtcaatttct gatgtctttc gtctcggact tcccctcgtt
     7921 tcgcgcaaat acggcgcgaa ggatgaggca cccagttact ccagcaccca atctttggcg
     7981 gccttgctta atgggacgaa gtcactttct ttgcgtgctt ccgaatacac tcgcaccacc
     8041 ggctcggtcc ccgataagcg ataacagacc cacgacccgt cttcaagaat aagcttcagg
     8101 ccgtcggtgc ggaccacctg catgactttg cgcccgccca gttcgtgcgg atcgcgcttc
     8161 actttttcag taaatttttc ttttacttcc ggagtcaacc ggaagttctc tcttaatgga
     8221 tagaaggaac caactttggc aaataattcc ttcaattgct ccccaatgct cctgccgcgg
     8281 acggccacca tctctgcaat cagtagccct gcgatgattc catctttttc gggaacatga
     8341 cctcggatgg tcagcccggc gctctcttcc ccgccgatgg cgattttgtc ctgatcaatc
     8401 agcgcgccga tgtatttgaa gcccacgggc gtttcataaa gctggacgtg gtgatggtct
     8461 gcgagcgcgt tgatgagatt agtggttgca acggacttgg ccacaccgtt tttccaaccc
     8521 cgtgtctcga ccagataatc aaaaagcagc gcaatgatgt agttcggctg gataaatgcg
     8581 ccatcgccgt ctacaacacc gaagcggtcg gcgtcgccgt cggtggctat gccaattgcc
     8641 gcgcctgttt ccttcatctt tgccttgcaa tcggcgagca ggtggtcgtc cggctcaggt
     8701 gcgtggcctc caaagagcac gtcgcggtaa tcatgcactg tggccacaga gacaccattt
     8761 tctgccagaa gctgtgacgc atagccgcgc cctgctcccc agaaggggtc gcagactatc
     8821 ttaatgccag ccttctgaat agctttgaga tctaccagtt cggccaggcg cttcaggtag
     8881 tcgggcctga cgtcagtttc ctcgaatttc tgcttctgcc cagggggcgc tgctacaaaa
     8941 cccaggtccg ctatgaaagc ctcaatctgg ctggtgactt cgggcagagc tggcgccccg
     9001 tcaggcgtgg agtacttgat gccgttatat tccggcggat tatgcgaggc ggtaaagttg
     9061 atggagcctc cggtcttgcg cgtgcgcacg gcataggcaa tcgcaggagt tggtgcggcc
     9121 tccggaatta cgatcggcgc gacaccattg gcagcgagta ctttggcggc ttcggctaca
     9181 aatatttcgc ccataaaacg cgggtcgcgc cctatgagaa cggtcttcgc tccaggctgt
     9241 gatgcgacat attttgcaat cccgctaaca gcgcggcgga cattggcaat cgtaaactct
     9301 tcggcgacaa tggcacgcca gcctgatgtt ccaaacttga tctgtgtggc catgattctc
     9361 ctgttctaag atgcacaaag aagaccagtc attgtcatct taaactctgg aaatttttat
     9421 ggaacaggcg cgcatcattc tcagcacaac atcttcgcgc gaagaggcgc agtgcatcgc
     9481 ccactctctg gtagacgagc ggctcgcagc atgcgtgaat atcatggatg gcataagctc
     9541 cgtttaccgc tggcaaggcg ccgtggaaga agccccggaa atacttctca tcatcaagag
     9601 cagcgtggaa aagcttgagg ctctggaagc ggcgcttcac aggctgcact cgtatgaagt
     9661 accggaattt cttgttttac cggtggaggg tgggagcagg gcgtatctgg aatggatacg
     9721 tgaatcggtg aaatagggac cctccgaaga aaaagcaggc ccttcatccc cttcgcgcaa
     9781 ctcagggtct taaggatgac aaaatttgag gggagataca cccggctcgg gatgataatt
     9841 tttcgggagg gagccaccca gaagctttag gaaacgcaca gcggggtttg ttattctcta
     9901 catggttcca tgcgtgtgct ttttgcagta ttcgtcctga gtatcgttgc gattatctgg
     9961 acggcggtgg cggtggcgcg ccacgtccgg aggaacggca gccagggcga gcgcatatcc
    10021 attaaggcct cagagccgga ccatgaacag gaataggaga gtcacttatg gcgcagagca
    10081 atgcaggttc aatcgttcta aattctactc gcgcaaatgc cgtgttgcgt tggggaacgg
    10141 ccatagtagc cggcagcgcg ctggttgcgg tgtgcgcgca catttccgtg cctctgtttt
    10201 tcacgcctgt tcctatgacg ttacagccat tcgccgttct gctgctgggt ctgtttatgg
    10261 agccggcagc ggccttcgcg gcgcttgtgc tgtatctggc agaaggcgcg gcgggcctgc
    10321 ctgtattcac tccgcagggc ctgggtggaa ttgcgcagat tcttggccca accggcggat
    10381 atctgatgtc gtatccattt gtggccgcct tggtcagctt tctttatcgt cgtctgcctg
    10441 tgcgtaactt ctggacggcg ctcatctcca cggcatcggg caatgcattg attctggcgg
    10501 caggcgcgac atggctggca gtattgaccc atcaaaccct gggccatacg gcaacgctcg
    10561 cggtaatgcc cttcttggcc ggagacgcat tgaaggtatg ccttgctgcg gccattgctg
    10621 ccggatggct ccgtatgcgt agaatagaaa agacggttgt ccgctgaccg taccctctcc
    10681 tgttttgatc cataagagca ccagggaaga acaagtgtgc ctcaatatac gtagcagtag
    10741 aaaggaatgt tttcgcagtg accgcatccg cttcctcgaa ggttgaatcc tccgacccgc
    10801 atcacagcca gatacgcgag atcaccatcg cgcacagccc cgattctgac gatgctttta
    10861 tgttttatgg gctggcgacc aataaggtcc gcgt
//
LOCUS       DASRBV010000046         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_662314, whole genome shotgun sequence.
ACCESSION   DASRBV010000046 DASRBV010000000
VERSION     DASRBV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662314"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFA02_02050"
     CDS             <1..925
                     /locus_tag="VFA02_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZU08659.1"
                     /translation="RIIDAMYEMGVQPERVMKLSGIGCSSKSPAYFLSRAHSFNSVHG
                     RMPSVATGALLANKTMLALGVSGDGDTASIGMGQFVHLLRRNLPLIYIIENNGVYGLT
                     KGQFSATADIGSKLKTGVINDLPAIDTCALAIQLGATFVGRSFSGDKKQLLAMLKAAI
                     AHKGTVMLDVISPCVTFNDHEGSTKSYKYMQEHEEAISEVGFVPHFEDISIDYDPGST
                     YDVTMHDGSHLRLRKLHEDYNPTDRVAAVKALMEASQRGEVLTGVFYIETQKPSFTEL
                     LNLVDEPLGTLPQERVRPSKQVLEEIMAKLK"
     gene            1021..1533
                     /locus_tag="VFA02_02055"
     CDS             1021..1533
                     /locus_tag="VFA02_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08660.1"
                     /translation="MKKLYLLLCGVLLLPVSALTQTAVYANFSASDFDLPHVGWQYGP
                     TFGIYHDAWHLPFAGVGVDGRASLIGSGSTKVYSGLVGPRAQVRPHVVPFQPYVEALI
                     GGGNVQVGQGVARTDKNVFEYQVVAGGDLTVLPHIDWRVAEFSYSGFSGMDQSFHPRT
                     FSTGIVLRLP"
     gene            1664..1737
                     /locus_tag="VFA02_02060"
     tRNA            1664..1737
                     /locus_tag="VFA02_02060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1696..1698,aa:Gln,seq:ctg)
     gene            complement(1998..4088)
                     /locus_tag="VFA02_02065"
     CDS             complement(1998..4088)
                     /locus_tag="VFA02_02065"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZU08661.1"
                     /translation="MKQVRNWIFRGTRSGIGSKVLACGMLLSVALQPSSAQSASGHPN
                     WPGPGQLFVGTCYQPIERTPAEIDRDIALMKQSGFRVVRMGDLSWDSFEPEQGKFDFA
                     WFDKIVDKMQANGIRVILDIPGTPAPIWLHRAYPGVDIVAEDGKRMPPAERYMDDISD
                     PDYVREVGILAEAMMKHYAHNPAIIALGYDNEIGNGYMSYSEGDRQRFVAWLEKKYGT
                     IDALNQAWQTEWWSRRLNSFSQVYMPLASGPGPSESFLDLHRYWSDVAVARIEQLEAL
                     RRQYMPDVPTISNLWPDAERRGFDYLSSYKSYVSYGAEGFYPADPISGAFGALMMKGA
                     LPTPIWFNEFTAGGGGFYGTPGRSRMYAYLGLMMGAQGFLAWTFNSHLGGEEQALFGL
                     LDHDSTPSWKLKEWADIASEFRELTKYGFPRYTHPQVAIAYSFDSFFDSHPNGPSNTT
                     RQYFKPSYTDQVEGAFEPLFRANIDTAVINIGHDSLSPYKLVVVPADYVMDPASAKAI
                     RNYVSGGGTVLMTAFSAKVDEHGNWFDTPLPGLLSDVFGLRASAFYEVQQSLTFTLDG
                     RSVDTGVHEYEVPEPSTATVLARFTNTPGQTPAITVNRFGKGEAIYLATEPKPSAIGP
                     LLDSLYSRVGVQRGPQTPDGVYARVVDGRTLYVNTTDHQQRIPLSHAGKGLITGRLYH
                     GEIVLDPWQVDLVQ"
BASE COUNT          877 a         1200 c         1184 g          877 t
ORIGIN      
        1 gcgcattatt gatgcgatgt acgagatggg cgtgcagccc gagcgcgtga tgaagctctc
       61 cggcatcggc tgctcgtcaa aaagcccggc ctatttcctg tcgcgagcac acagcttcaa
      121 cagcgtccac ggacgtatgc cttcggttgc caccggtgcg ctgctggcca acaagaccat
      181 gctggcgctt ggcgtttccg gcgacggaga cacggcctcc atcggcatgg ggcagttcgt
      241 ccacctgctg cgccgcaatc tgccgctgat ttacatcatt gagaacaatg gcgtttatgg
      301 cctgaccaaa ggccagttct cggcaacggc cgacatcggc tcaaagctta agaccggggt
      361 catcaacgat ctgcctgcga ttgatacctg cgcgcttgcc atccagctag gcgcgacttt
      421 cgtaggacgc tcgttctccg gtgacaagaa gcagcttctc gccatgctga aggccgccat
      481 cgcgcacaaa ggcacggtga tgctcgacgt catctcgccc tgtgtgacct tcaacgacca
      541 tgaaggttcc accaagagct ataagtacat gcaggagcac gaagaggcca tcagcgaagt
      601 cggttttgtt ccgcacttcg aggacatcag cattgattat gatccaggct cgacctacga
      661 cgtgaccatg cacgatggct cgcatctgcg cctgcgcaag ctgcatgagg actacaatcc
      721 tacggaccgc gtggccgctg taaaagcgct gatggaagcc agccagcggg gcgaagtcct
      781 taccggcgtc ttctacatcg aaacgcagaa gcccagtttt actgagctgc tgaacctggt
      841 agacgagccg ctgggcacgc ttccgcaaga acgcgtgcgg ccctcaaaac aggtgctgga
      901 agaaatcatg gcaaagctga agtaattgca atcattaaaa tatgagggcc gcaacgttgc
      961 ttagcctgcg gcccttattc cttcatagaa ttacaatccc agatattttt tgaggtcagt
     1021 atgaagaagc tctatctctt gttgtgtgga gtgttgcttt tgccagtttc ggctttgacg
     1081 caaacagccg tttatgcaaa cttcagtgcg tcagatttcg acctccctca tgtaggctgg
     1141 cagtacggcc ctacatttgg catttatcac gacgcatggc acttgccctt tgcgggtgtt
     1201 ggtgtagatg gccgcgcatc cttgattgga tcaggttcaa caaaggttta tagcggactc
     1261 gtaggcccgc gtgctcaggt gcgtccgcat gtcgttccat tccagcctta cgtagaagca
     1321 ttgattggcg gaggaaatgt gcaggttggg caaggcgtag ctcgcaccga caaaaatgta
     1381 tttgaatatc aggtagtagc cggaggcgat ttgaccgtcc ttccgcatat tgactggcgt
     1441 gttgccgagt tttcttacag cgggttctcc ggcatggacc agtccttcca ccccagaact
     1501 ttcagcacag gtatcgtgtt gcgcctgccg taaaccagac cagcgccaga tataaagatg
     1561 agccaatgca aagagcagca ggaatttacc tgctgctctt gtgttgaccg gagttccgcc
     1621 agcctttact ataagaaaga ccaatctaag gttttctctg cactgccccc tcgttcaatg
     1681 gtaggacagc tgactctgga tcagcatatc ggggttcgaa tccctggggg gcagccaatt
     1741 agtgccggca cgcccgacct ggagtggaac tcgccgaatg gttgcagctg cagatcgaca
     1801 ccggcgcagg atgcgcctct ctcgtcgctg cgcgtgcagg ttcttcgggc gccggctgca
     1861 tagcccgggc caccacgcga taagccagca ggcagatgag ttacaaggcg gaggatggat
     1921 gtaaaggcgc agaggcgcgc cggtcaccgg ggcacccagt ggaggctgga taacatgggt
     1981 aaccgagtcg ctgcttctta ctggaccaga tcgacttgcc agggatcgag gacgatctcg
     2041 ccgtggtaga ggcggccagt gatcagacct tttccggcgt gtgacaaggg aatcctctgc
     2101 tgatggtccg tcgtgttgac atagagcgtc cgtccgtcaa ccactcgtgc gtacacgccg
     2161 tccggggtct gcggaccgcg ctggactcca accctggaat agagactgtc aagaaggggg
     2221 ccgatcgcgg atggcttcgg ctcggtggca agatagatcg cctctccttt gccgaatcgg
     2281 ttgaccgtaa tcgccggcgt ctgcccgggt gtattcgtga atcgcgcaag cacggtcgcc
     2341 gttgaaggct caggcacttc gtattcgtgc actcccgtat cgacagatcg tccatccagc
     2401 gtgaacgtca gggattgttg cacctcataa aaagcgctgg ccctgagccc gaagacatcg
     2461 ctgagaagcc ccggcagagg cgtgtcgaac cagttgccgt gctcatcgac tttggcggaa
     2521 aaagccgtca tcagcaccgt gcccccaccg ctgacgtaat tccgaatcgc ctttgcgctg
     2581 gcaggatcca tgacgtaatc cgccggcacc actaccagct tgtaaggaga gaggctgtcg
     2641 tggccgatgt tgatgactgc ggtgtcgata ttggcgcgaa ataggggctc gaaggcgccc
     2701 tccacctgat cggtatacga aggcttgaag tattgccggg tggtgttcga cggcccgttc
     2761 ggatgcgagt caaagaacga gtcgaaggaa taggcgatgg ccacctgcgg atgcgtgtaa
     2821 cgcgggaagc cgtactttgt gagttccctg aattccgaag cgatgtcagc ccattctttc
     2881 agcttccatg aaggggtgct gtcatggtcc aacaacccga agagcgcctg ttcctcaccc
     2941 ccgaggtggc tgttgaaggt ccaggccaga aagccctgcg cgcccatcat caggccaagg
     3001 taggcataca tccggcttct gcctggggtg ccgtaaaatc cgccgccgcc ggccgtgaac
     3061 tcattgaacc agataggagt gggcagggct cccttcatca tgagcgcgcc aaacgccccg
     3121 ctgatgggat ctgcagggta gaacccctcc gccccatagg agacatacga cttgtaactg
     3181 ctgaggtagt caaatcccct gcgttccgcg tctggccaga ggttggaaat cgtgggaacg
     3241 tcgggcatgt actggcgccg aagcgcttct aactgctcga tccgcgcaac ggcgacgtcc
     3301 gaccagtagc ggtgcaaatc aagaaagctc tcggatggtc cggggccgct ggccagcggc
     3361 atgtacacct gactgaagct gttcagccgc cgtgaccacc attctgtctg ccacgcctgg
     3421 ttgagcgcat cgatcgtccc gtatttcttc tccagccacg cgacaaagcg ctgccgatcg
     3481 ccttcggagt aagacatgta accgttaccg atctcattgt cgtagccgag ggcgataatg
     3541 gccggattgt gcgcgtaatg tttcatcatc gcttcggcca gaatgccgac ctcgcgcaca
     3601 taatccggat cactgatgtc atccatgtag cgctctgccg gcggcatgcg tttgccgtcc
     3661 tccgccacaa tatcaacgcc cggataggcg cgatgcagcc agatcggcgc cggcgtgccc
     3721 gggatgtcga gaatgaccct gatcccgttc gcctgcatct tatccacaat cttgtcaaac
     3781 catgcgaagt cgaacttccc ctgttcgggc tcgaaggagt cccaggagag atctcccatg
     3841 cgcaccacgc ggaagcccga ctgcttcatc aaagcgatgt cgcgatcgat ttccgcgggc
     3901 gtccgctcga tgggctgata gcaggtgccg acgaagagct gcccgggacc gggccagttc
     3961 gggtgaccgg atgcggactg agccgaactc ggctgcaacg ccacgctcag cagcattcca
     4021 cacgccaaca ccttcgatcc gattccggat cgcgttccac gaaaaatcca attgcgcact
     4081 tgcttcatgc tcgatctctg ccgatggcca gattcgcctg ttccagcaaa atctgaaa
//
LOCUS       DASRBV010000047         6678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_53000,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000047 DASRBV010000000
VERSION     DASRBV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53000"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..988
                     /locus_tag="VFA02_02070"
     CDS             323..988
                     /locus_tag="VFA02_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZU08662.1"
                     /translation="MSEKILAVLASFIIATISASGYAGVAFLMAIESACIPLPSEIIM
                     PFSGYLVHTGQLKLFWVATAGAIGCNLGSVVAYWIGAWGGRPFIDKYGRYVLMNHHDL
                     ERAEHFFHRFGGITVFLGRLLPVVRTFIALPAGIGRMPQLRFHIYTFLGSWPWCYALA
                     WFGMKLGQAWETDLRFKAAFHRFHLAVEILLLVGVVWFVWTHWRNRVGAGREAEEARA
                     SKG"
     gene            1138..2466
                     /locus_tag="VFA02_02075"
     CDS             1138..2466
                     /locus_tag="VFA02_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZU08663.1"
                     /translation="MSKPKAEATQVQPAKGKLGVMVVGMGSVATTLIAGVEAVRKGLA
                     KPFGSLTQMGTIRLGKRTDARTPLIKDFVPLADLNDIVFTGWDIFPENMYEAAEHAAV
                     LDRDHLKSIRPFLEAIKPMPAVFDQHYVKRLHGTHVKKGKSKCDLAQQVRSDIQAFKA
                     QVDRVVVIWCGSTEIFLEPSEVHATIESFEKGLVNDDPNIAPSQIYAYAALKEGVPFA
                     NGAPNLTVDLPCMQQLSKQTGAPICGKDFKTGQTFMKTVLAPAFKARMLGVSGWYSTN
                     ILGNRDGEVLDDPESFKTKEESKLGVLEYILQPELYPDLYKDIYHKVRINYYPPRGDN
                     KEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLALDLVLFLDLAQRTPELKHLGIQE
                     WLSFYFKSPMVAEGLYPEHDLFIQSMKLKNTLRHIMGEDLITHLGLEYYD"
     gene            complement(2537..2965)
                     /locus_tag="VFA02_02080"
     CDS             complement(2537..2965)
                     /locus_tag="VFA02_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU08664.1"
                     /translation="MSRIFWDTNLFIYLFEDYGKHSRAVENLRRRMLERGDQLVTSSL
                     TIGEILTKPHSVGNHLACEEYEEAIRATALVVNFDILAARNFAKLRAQNEINIRPPDA
                     IQLACAATAGVDLFLTNDTHLHHVHVDGIHFITSVDRAPL"
     gene            complement(2962..3462)
                     /locus_tag="VFA02_02085"
     CDS             complement(2962..3462)
                     /locus_tag="VFA02_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08665.1"
                     /translation="MAIYIAKTGRSFLAADRVWIATALLHKENPKRTGFSLNEICEKA
                     RQEGFLEETTNTFYLHANQHCVANRTPNPGNHRMLFEPSRGERRLYRPGDLCKPGRVG
                     KITPNPDDIPPKYRYLLDWYNDWSKRSAPQPPLDRLLQLKGSGKDLWKDEHADEYVDR
                     LRKGWE"
     gene            3692..>6678
                     /gene="dnaE"
                     /locus_tag="VFA02_02090"
     CDS             3692..>6678
                     /gene="dnaE"
                     /locus_tag="VFA02_02090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZU08666.1"
                     /translation="MAEFTHLHLHTDYSLLDGACDVEKLVNRVADIGQKAVAITDHGN
                     IYGAVHFFEAAKKRGVKPILGCELYICKKEDHRDDPQGDDYNHLLVLAENEEGYRNLV
                     RITSEASLYGFYRKPRVSKKYLAEHAEGLIGFSGCLAGELCENLMAGKYEAAKAVAGQ
                     YQDIFGKGNFFLEIQDQGLELEKKIHPDLFRLEKDLGIPLVATNDSHYLCDDDAHAHE
                     ILLCVQTASSIHDPKRFKFDSDQFYVKTAAEMEKLFSHAPEVVSRTMQFAERCNLKLS
                     KVDNPFPEFAVPPGHTIDSYFEHVCREGFKKRLETSIRHLQDRGLLRHPISEYEARLE
                     REISIIRQMKFPGYFLIVWDFIRYAREHNIPVGPGRGSAAGSLVGYVMEITNIDPLQN
                     ELLFERFLNPERISMPDIDIDFCMNRRGEVIDYVTRKYGREQVAQIITFNTMAAKAAI
                     KDVGRALDMPYGDVDRIAKLVPTTIGVTIEQALQDSPQLQEAYDTNPQVRELIDTAKK
                     LEGLVRGAGVHAAGVVIAPRPLTDLVPVSRSKNDEIVTAYDMKAVEKMGLLKMDFLGL
                     TTLTVIDDCLKMIERNQGQKLDIEKVPLDDQETYEKVFHRALTSGVFQFESGGMRDVL
                     RRYKPTTVEDLTALNALYRPGPIQGGMIDDFIERKWGRRKVEYELPELEAILKETLGV
                     IVYQEQVMQIANVLAGYSLGEADLLRRAMGKKIPEEMAKQRDRFVSGALEKGFPKDKI
                     TKIFDLMEQFAGYGFNKSHSAAYALLAYQTAYLKTHYPVEFMAALLTSEISKPENVVK
                     YIKECREMEIPVEPPDVVYSDADFTPHGKAIRFGLTAIKNVGRNAIDSILTARRELEE
                     QGKKFSSFWEFCEKVDLRLMNKRVIESLIKAGALDSFGRRAQLMAAVDKAIERAQKTQ
                     RDLEAGQHGLFGIFDDHPSVSAKADELPNVPDWDEHQRLQFEKEVLGFFVSGHPLDKY
                     YEKLRNLPGVIDTATALEMKPAP"
BASE COUNT         1519 a         1675 c         1930 g         1554 t
ORIGIN      
        1 acggagggtg ctcctccaat gcgttcatag attgtgcttt cgggcatgac tcacacctcg
       61 gcttctgagt tgaaaaatgg aattgggaac aaaatccaga ccgggcgcat cggggcgggg
      121 tcaatgctgg aaagccatcg caggcaaaac tggcgcatcc tggcgctgtg cgatgacctg
      181 gttctgaaac cagaatcaca cgggaatgct gatactgcac aaaaaggctg gagggcggcc
      241 ccagatttat tgcgtgagta aacctcgttt atggaccgtc atccgcatcc tttaagagtc
      301 tgccggatat actcaaagct gaatgagtga aaaaatcctg gccgtcctgg cttcctttat
      361 tattgccacg atctccgctt ctggttatgc aggggtcgcc tttcttatgg cgattgaatc
      421 ggcatgcatt cctctgccgt ccgaaatcat catgcccttc tccgggtatc tggtgcatac
      481 cgggcagttg aagctgttct gggtggcaac agccggtgcg attggctgta atcttggctc
      541 agtggtggcg tactggattg gtgcgtgggg cgggcggccg tttatcgata agtatggccg
      601 ttatgtactg atgaaccatc atgacctgga gcgtgcggag cacttcttcc atcgctttgg
      661 cggaatcaca gtgtttctgg ggcgactgtt gcctgtcgtc cgtaccttta ttgcgctccc
      721 tgcaggcatt ggcaggatgc ctcagcttcg cttccacatc tatacctttc tcggatcatg
      781 gccgtggtgc tatgccctgg cctggtttgg aatgaagctg ggacaggcgt gggaaacgga
      841 ccttcgtttc aaagcagctt tccatcgctt ccaccttgcc gtggaaattc tgctgctcgt
      901 tggcgtggtc tggtttgtgt ggacgcactg gagaaaccgt gtgggagcgg ggcgcgaggc
      961 agaagaggca agggcgagca aaggatagcg gttcagtgtg gaagtatttc cattcgcagt
     1021 gcgcattttg ccattaacat tctctgattt gccaaacctg caggcaaaag cacaaactga
     1081 agcctttggt gcagtccacc actttatctg agatagaagg acggttgaag caaggaaatg
     1141 tctaagccca aagctgaggc aacacaggtc cagccagcaa aaggaaaact gggagtcatg
     1201 gtcgtcggca tggggtcagt ggccacgacc ctcatcgccg gagtggaagc ggtccgcaaa
     1261 ggattggcga agccttttgg ctcgctgacg caaatgggta cgatccgcct cggcaagcgc
     1321 acagatgcgc gtactcctct tatcaaggat tttgtccccc tggcagactt gaatgacatt
     1381 gtctttaccg gatgggacat ctttccggaa aacatgtatg aggcagccga gcacgcggcc
     1441 gtgcttgacc gcgaccacct gaaaagcatc aggccgtttc ttgaggcaat caagcccatg
     1501 ccggcggtct ttgatcagca ctacgtcaag cgcctgcatg gcacgcacgt aaagaagggc
     1561 aagagtaaat gtgacctggc gcagcaggtg cggtcggaca ttcaggcctt caaggcgcag
     1621 gtagaccgcg tggtggtgat ctggtgcggt tctacagaga tttttcttga gccgtcggag
     1681 gtccacgcca cgattgagtc gtttgagaaa gggctggtga atgacgatcc gaacattgcg
     1741 ccttcgcaga tttatgctta cgctgcgctg aaggaaggcg ttccgtttgc caatggcgcg
     1801 ccgaacctta cggtggacct gccgtgtatg cagcagcttt caaagcagac aggcgcgcct
     1861 atctgcggca aagacttcaa gaccgggcag acattcatga agaccgttct tgctccggcg
     1921 ttcaaggcgc ggatgttggg tgtttccgga tggtattcga cgaacattct cggcaaccgg
     1981 gatggtgaag tgctggacga tccggagtcc ttcaaaacaa aggaagaatc gaagctcggt
     2041 gtgctcgagt atatcctgca accggagctc taccccgatt tgtataagga catttaccac
     2101 aaggtccgca tcaactatta ccctccgcgc ggggacaaca aagagggctg ggacaatatt
     2161 gatatcttcg ggtggctcgg ctatccgatg cagatcaaag tggacttcct ctgccgtgat
     2221 tccatcctgg cagcaccgct ggcgctcgac ctggtgctgt tcctggatct ggcgcagcgt
     2281 acgccggagt tgaagcacct cggcattcag gagtggctga gcttctactt caagtcaccg
     2341 atggtggccg aagggctgta tccggagcac gacctgttca tccagtccat gaagctcaag
     2401 aacacgctcc ggcacatcat gggcgaggac ctgatcacgc acctcgggct cgagtactat
     2461 gactgacgag ttgtaatggc ccccaaatgg caggtcgtgc attgcagacc tgccattttt
     2521 ctttgcgcga acaattttaa agaggggcgc gatcaaccga agtgatgaaa tggatcccgt
     2581 ctacgtgtac gtgatgcagg tgggtgtcat ttgtaagaaa aagatcaact ccagcagttg
     2641 cggcgcaagc aagctggatc gcatcgggcg ggcgtatgtt tatttcattt tgcgcgcgca
     2701 actttgcgaa attgcgtgcg gcaaggatgt caaaattgac cacaagtgcc gttgcgcgga
     2761 tggcttcttc atattcctca caggccaggt ggtttccaac agagtgtggt tttgtcaata
     2821 tttctccgat tgtcagggaa gaagtcacga gctgatctcc ccgttcaagc atcctgcgcc
     2881 gtagattctc taccgccctg gaatgtttgc cgtaatcttc aaacaggtag ataaagagat
     2941 ttgtgtccca gaagatacgg ctcattccca tcccttccgc aaccggtcaa catactcatc
     3001 tgcatgttcg tccttccaga ggtcttttcc cgaacccttc aattgaagga gcctgtcgag
     3061 gggtggttga ggagccgagc gcttgctcca atcgttgtac cagtcaagca ggtagcggta
     3121 ctttggaggg atgtcatcag gattgggggt gattttcccc acgcgtcctg gcttgcagag
     3181 atctcctggc cgataaagcc tccgctctcc acgtgatggc tcaaagagca ttctgtgatt
     3241 tccgggattg ggcgttcggt tcgctacgca atgttggttt gcatggagat agaaggtgtt
     3301 cgttgtttcc tccaggaatc cctcctgtct cgctttctcg caaatttcat tcaggctgaa
     3361 gccggtacgt ttcggatttt ctttatggag cagagccgtt gcaatccaga cacgatctgc
     3421 tgccagaaag ctcctcccgg tttttgcgat gtagatggcc atcagataac cctcttctgt
     3481 ttatatttaa ctttatcgta cgataaataa tctgtcaagc ataattttga taatcagaaa
     3541 atgcctaaag agcagcgggg agttccctac cgatagatac agatcggggg agccaaaaac
     3601 tgccctgctt ttcctcatcc agacacagat ttgcacctat cctgctgatg aatacccgct
     3661 ctcagcagaa ctaaaataga gccatactct catggctgaa tttacccacc tgcacctgca
     3721 tactgactat tcgcttctgg atggcgcctg cgacgtggag aagctggtca accgtgtggc
     3781 cgacatcggc cagaaggctg ttgccattac cgaccacggc aacatctatg gcgcggtgca
     3841 tttttttgag gcggcgaaga agcgcggcgt gaagccgatt ctgggatgcg agttgtatat
     3901 ctgcaagaaa gaagaccacc gcgatgatcc gcagggtgat gactacaatc acctgctggt
     3961 gctcgcggag aacgaagaag gctatcgcaa cctggtccgg atcacctccg aggcgtcact
     4021 ctatgggttt tatcgcaagc cgcgtgtgag caagaagtat ctggcggaac atgctgaagg
     4081 attgatcggg ttttccggct gccttgccgg cgagctgtgc gaaaatctta tggcagggaa
     4141 atacgaagcg gcgaaggcgg ttgcagggca gtatcaggac atcttcggca aagggaactt
     4201 ctttctagag atccaggacc agggactgga acttgagaag aaaatccacc ctgacctgtt
     4261 ccggctggag aaggatctgg gcattccgct cgttgccacg aatgacagcc actatctctg
     4321 tgatgatgat gctcatgcgc atgagattct gctgtgcgta cagaccgcct cttcgattca
     4381 tgatcccaag cgcttcaaat ttgattccga tcagttctat gtgaaaacag cggcggagat
     4441 ggagaagctt ttctcacatg ctccggaagt cgtcagccgc acgatgcaat ttgccgagcg
     4501 ctgcaatctg aagctcagca aagtggacaa tccgtttcct gagtttgctg ttcctcccgg
     4561 gcatacgatt gacagttatt ttgagcacgt ttgccgcgaa ggattcaaaa agaggcttga
     4621 aacttccatt cgccatttgc aggaccgtgg tctgctgcgc cacccgattt ctgagtatga
     4681 ggcgcggctg gagcgcgaaa tttccatcat caggcagatg aagttcccgg ggtacttcct
     4741 gattgtgtgg gacttcatcc gttatgcgcg tgagcacaac attccagtcg gccccgggcg
     4801 tggctcggcg gcgggatcgt tggtcggcta tgtgatggag atcacgaaca ttgatccgct
     4861 gcagaatgag ctgctgtttg agcgcttcct gaacccggag cgcatctcta tgccggacat
     4921 tgatattgac ttctgcatga accggcgcgg agaggtgata gattacgtta cgcggaaata
     4981 tggacgcgag caggtggcgc agatcatcac cttcaacacg atggcggcaa aggcggcgat
     5041 caaagacgta ggccgcgcgc tcgatatgcc gtatggcgac gtggaccgca tcgccaagct
     5101 ggttccgacc acgatcggcg tgacgattga gcaggcgctg caggattcac cacagttaca
     5161 ggaggcatac gacacaaatc cgcaggtgcg cgagctgatt gacacggcaa aaaagctgga
     5221 agggctggtc cgcggagcag gcgtccacgc agcgggtgtg gtgattgccc cgcgtcctct
     5281 gaccgacctg gtccctgtgt cacggagcaa gaacgacgaa atcgttacag cgtatgacat
     5341 gaaggcggtc gagaagatgg gccttcttaa gatggacttt ctcggcctga caacgctgac
     5401 ggtgattgat gattgcctga agatgatcga gcgtaaccag ggccagaagc tggatatcga
     5461 aaaggtcccg ctcgatgacc aggagacgta tgaaaaggtc tttcatcgag cgctgacatc
     5521 gggtgttttc cagtttgaat caggtggaat gcgcgatgtg ctgcggcgtt acaaacctac
     5581 gacggttgaa gatctgacgg ccctgaatgc gctctaccgt ccggggccca tccagggcgg
     5641 catgattgat gacttcatcg agcgcaagtg ggggcgcagg aaggtcgaat acgaactgcc
     5701 ggaactggaa gcaatcctga aggagacgct cggcgtcatc gtctaccagg aacaggtgat
     5761 gcagattgcc aacgtgcttg caggttactc gctgggcgaa gccgatttgc tgcggcgcgc
     5821 gatgggcaag aagattccgg aggagatggc gaaacagcgc gaccggtttg tttccggcgc
     5881 gctggaaaag ggcttcccga aagacaagat caccaaaatc ttcgacctga tggagcagtt
     5941 tgccggatat ggctttaata agtcgcactc tgctgcgtac gcgctcctgg cttatcaaac
     6001 ggcgtatctg aagacgcatt atcctgtcga atttatggcg gcgctgctga cttcagaaat
     6061 ctcgaagccg gagaacgtgg tcaagtacat caaggaatgc cgcgagatgg agatccccgt
     6121 cgagcctccg gatgtggttt acagtgatgc cgattttact ccgcatggca aagcgattcg
     6181 ttttggtctg actgccatta aaaacgttgg gcgcaatgcg attgattcga ttctaacggc
     6241 gcgcagggaa cttgaagagc agggaaagaa gttctcttcc ttctgggagt tctgcgaaaa
     6301 agttgacctg cggctgatga ataagcgcgt catcgagtcc ctcatcaagg ccggggctct
     6361 cgattctttc gggcggcgcg cgcagttgat ggctgcggtg gacaaggcca tcgagcgggc
     6421 acagaagacg cagcgcgacc tggaagcagg acagcacggg ctgtttggga ttttcgatga
     6481 ccatccctcg gtttctgcga aagcagatga attgccgaat gtacctgatt gggacgagca
     6541 ccagcgtctc cagtttgaga aagaggtgct tgggttcttt gtctccggac atccgctgga
     6601 caaatattac gagaaactgc gcaatttgcc gggcgtgatt gacacagcaa cggcgcttga
     6661 gatgaaacct gcccccag
//
LOCUS       DASRBV010000048         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_306440, whole genome shotgun sequence.
ACCESSION   DASRBV010000048 DASRBV010000000
VERSION     DASRBV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306440"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VFA02_02095"
     CDS             <1..1168
                     /locus_tag="VFA02_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU08667.1"
                     /translation="LIAWIIGWDLILEYAFSNMAVCVGFAAHIVDLMDWLGIHPSARW
                     ISPAYLPSGLQDFHGNTLYAPGWHFGFNWPAFLIVMILTVVLVRGIRESAETNNVMVL
                     LKIAAILIFIAFGAHFVHPSNWHPFSPNGWSGLLTGGSIIFFTYIGFDSVSTAAEECR
                     NPQRDVPIGILATLLICTVLYIGVAVVLTGLVKWDTLVGDAAPVVNTLRKLSATNHSE
                     TLHWGRLVILIGALLGMISSILVFQIGQARVWFAMSRDRLLPAVFSKVHPRYRTPAVS
                     TWVAGIVVGLPAGLLDIGTFSDLSNIGTLFAFVLVSAGVLILRYKDPDRHRSFRCPGG
                     PVFPVLSIFFCVLLMAGLPIMTWLRFFIWLGIGLVIYFFYSRRRSEFCPSERTR"
     gene            1298..2308
                     /gene="add"
                     /locus_tag="VFA02_02100"
     CDS             1298..2308
                     /gene="add"
                     /locus_tag="VFA02_02100"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZU08668.1"
                     /translation="MSDSLRTFLRRLPKAELHLHLEGTITPGTLVELSRRHDAEPLTP
                     AQAEALYQYTDFTGFLMAFKAVSERLRTPEDYELITYRMAGQLAAQGIIHAEVYVAVG
                     VVYFWGHTEFEPLFAGMERARIKAERDFGISICWIFDAVRHFGVEPAARVFRKAAEMR
                     REHSSIIGIGIGGDERRGPADLFRDLYAEARDAGLRLTVHAGEAAGPESIWGALNLGA
                     ERLGHALTAIHDPELMHVLAERQVPVEVCVTSNLRTGCCTQLPEHPVRKYFDSGLMVT
                     LNSDDPAMFGSNLEEEFLLAHNEFGFTQEQLRELAANSIEASFLPAEQKVRWLHQIEQ
                     SV"
     gene            2301..2630
                     /locus_tag="VFA02_02105"
     CDS             2301..2630
                     /locus_tag="VFA02_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08669.1"
                     /translation="MSKEIRSAADAHYNSEMRYDVGMKPLKQRLFRSGKKAEVIVLPG
                     LRKKDESAIALKAIGWTSVAVGVVALGIYVGRELRLRYKFNRRTPYDFYSHAGDQISA
                     AEYGVGV"
BASE COUNT          574 a          776 c          816 g          730 t
ORIGIN      
        1 gctgattgcc tggatcatcg gctgggacct gattctggaa tacgccttct ccaacatggc
       61 agtgtgtgta ggttttgccg cccatattgt ggacctgatg gactggctcg gcattcaccc
      121 atcggcacga tggatttcgc ccgcctatct tccaagcggt ctgcaggatt ttcacggaaa
      181 cacgctttat gccccggggt ggcacttcgg ctttaactgg ccagcgtttc tcatcgtcat
      241 gatattgacg gtggtgctgg tgcgcggcat tcgcgagtcg gctgagacga acaatgtaat
      301 ggtcctgctg aagattgcgg ctatccttat ctttattgcg ttcggagcac actttgtcca
      361 tccgtcgaac tggcacccgt tttctcccaa cggctggtcc gggctgctga caggcggctc
      421 cattattttc tttacctata ttgggttcga ttctgtttcg actgcggcag aagagtgtag
      481 aaatccgcag cgtgatgttc ccatcggcat cctcgccacg ctgttgattt gcacggtgct
      541 ttacatcgga gtggccgttg tactgaccgg gctggtcaag tgggacacac tggttggcga
      601 tgccgctcct gtagtgaata ccctgcgcaa gctctctgca accaaccaca gcgagacact
      661 gcactgggga cggcttgtca ttctgattgg cgcactgctc ggcatgatct catccattct
      721 ggtcttccag attggacagg cccgtgtatg gttcgccatg tcgcgtgacc gccttctgcc
      781 cgcggtcttc tcaaaggtgc atccacgcta ccgtacgccc gcggtttcta catgggtggc
      841 aggcattgtt gtcggccttc ccgccgggct gctagacatt ggcacattct ctgacttgtc
      901 aaacattggc acgctctttg cttttgtgct ggtatcggca ggtgtgctga ttctccggta
      961 caaagaccct gaccgtcacc ggagctttcg ctgtcctggc gggccggtct ttcctgttct
     1021 gagtattttc ttttgcgtgc ttctgatggc gggtcttcct atcatgacct ggctgcgctt
     1081 cttcatctgg ctgggaatcg ggttggtcat ttatttcttc tacagtcgca gacgcagcga
     1141 attttgtccc tcggagcgga caagataagg gccgctgttt tgcacatgaa ctgtaccact
     1201 tcaggaccgg gtttccttca aaagtgtttt tgcgccagag ccatgaatgg agggacagag
     1261 aagtataaac cgtgtcatcc aaagggtttg aaaaggcatg tctgactcac ttcggacttt
     1321 tctccgccgt ctccctaagg cggaactaca tcttcatctt gagggaacca ttacgcccgg
     1381 cactcttgtg gaattgagcc gcaggcatga tgccgaacca ctcacgccag cgcaggccga
     1441 ggccctgtat caatacaccg acttcacggg ttttctgatg gcatttaagg ccgtctctga
     1501 gcggctgcgc acgccggaag attatgaact gattacctac aggatggccg ggcagctcgc
     1561 cgctcagggc attattcatg ctgaagtcta tgtggctgtc ggcgtggttt atttctgggg
     1621 gcacacagaa tttgagccgc tcttcgctgg aatggaacgc gcccgcatca aggccgagcg
     1681 agacttcggt atcagtatct gctggatctt cgatgctgtc cggcattttg gtgtagaacc
     1741 agccgcgcgt gtcttccgca aagccgccga gatgaggcgc gagcattcca gcattatcgg
     1801 catcggcatc ggcggagacg agcgccgcgg gcccgccgat cttttccgcg acctgtatgc
     1861 agaagcgcgc gatgccgggc tgcgcctgac cgtccatgct ggcgaggcag ccgggccgga
     1921 gagcatctgg ggcgcgctga atcttggcgc cgaaaggctg gggcatgcgc tgacggcaat
     1981 ccacgatccc gaattgatgc acgtgctcgc cgaacggcag gtcccggtgg aggtctgcgt
     2041 gacatcgaac ctcaggacag gctgctgtac ccaacttccg gaacatcccg ttcggaaata
     2101 ttttgattcc ggcctgatgg ttacactgaa ctcggatgac ccggccatgt ttggcagcaa
     2161 tctcgaagag gagttcctgc tggcgcacaa cgaatttggc tttacacagg agcagctgcg
     2221 cgaactggca gccaattcca tagaagcaag tttccttcca gcggaacaaa aagtgcgctg
     2281 gttacatcaa atagaacaga gtgtctaaag agatacgtag tgccgccgat gcgcactata
     2341 acagcgaaat gcgctatgat gtcggcatga agccattgaa acaaaggctt tttcggtcgg
     2401 gaaagaaggc tgaggtgatt gttctaccgg gtctgagaaa aaaagatgag tctgccatcg
     2461 cactcaaagc catcggctgg acttccgttg cagttggagt agttgccttg gggatatatg
     2521 taggccgtga gctgcgtctg cggtacaaat tcaaccgtcg cacgccgtat gacttctact
     2581 cacatgccgg cgatcagata tctgctgccg aatatggcgt gggcgtctga ttttttgcct
     2641 gcctgctgcg tgctgccttt caggtagatt tgtgtcccgc atcctgcaaa ctgcacaagt
     2701 aaatttctga gcggaaaaag ccctcgaagg atgggctttt ctttccattc tcctggccgt
     2761 tccgggaaga aattcacgct atcattgcat accaatggag agataactgc tgtcgctgca
     2821 tagcgaggcc gtgcctggga tgaaaatgat tgtcagtgcg cttgccggat ttctggccct
     2881 gggtgcaatc caggca
//
LOCUS       DASRBV010000049         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_206842, whole genome shotgun sequence.
ACCESSION   DASRBV010000049 DASRBV010000000
VERSION     DASRBV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206842"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1712
                     /locus_tag="VFA02_02110"
     CDS             531..1712
                     /locus_tag="VFA02_02110"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HZU08670.1"
                     /translation="MAILFLSGVSRAEDQGARDRAYSVEVMQRIAAPVLTAISQNKLK
                     QTMPVEGPPDRASYTHLEALGRLLAGMAPWLELGPDNTSEGKLRARDIQLAVQAISHA
                     VDPKSPDSMNFDKGQQPLVDTAFLALALLRAPKQLWGNLGAVDKANVVAALKKTRAIH
                     PFESNWLLFSATIEATLWKFTGECNQTAIEYAINRHMEWYKGDGVYGDGAHFHWDYYN
                     SYVIHPMLLEVLRICAEKQHPLGKLYLVELERAQRYAVIQERLIAPDASFPVMGRSSA
                     YRFGAFQTLSDIALMRQLPPSLDPGAVRSALTAVIRRMIETPGTFDAHGWLRIGAVGH
                     QPHIGEGYISTGSLYLCTAGLLHLGLPPDDPFWTAPARPWTQKQIWAGEDVPADHAIA
                     N"
     gene            1843..>2576
                     /locus_tag="VFA02_02115"
     CDS             1843..>2576
                     /locus_tag="VFA02_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08671.1"
                     /translation="MSTNLVTALTVMLEKAAREAGLTVLSADAGTDFNGHPTAIFKLA
                     LSPDAAPERTLQLELSDAFDFQRPDLLPEMTSHLREEAKRLRNPRPNTYVTLAGLPIR
                     FSGFGWPFHRSTSGADTYIVHGVVHLEDGTNSPLHAKVSASMTVTFAEIVPAPEQPYA
                     ETFIYNAIRKTFDQGQLELLKSGNRQPVPVTTRYYSRWQKKFFFTDTDDNSRMEFLKL
                     KTYWLSGVLGNGQPVWIADPRDAQYL"
BASE COUNT          556 a          808 c          698 g          514 t
ORIGIN      
        1 ttgcctgtgc tgaaactctc agagaacccg cccagaagac cgcaaacagc gcgatcagca
       61 aacatatcag gtcccgctta tgctcatgtt ttttaacatg gcttgtttga aatgtagtgc
      121 tcatgcttag cctccatacc ccaaatccaa gtaccgcttc agtgctgctg gtcagtccag
      181 taaacgtatt ccacgtttta ctgctgctta aggtcaccga tgactgcttc gttttactaa
      241 agcgttttat gggtcgtgag cgagattctg tcggcgcgag aatacgattg tcaagaaaaa
      301 aaatgaatcc tccgcgccag cattggaagc gttagacttc cgacccacat tccgggccgg
      361 gggcacagag ggcaaggagt tgcaaccgaa atgcaccctt ttccgcgcag ccatccgcaa
      421 aaggcccgat caggaccttt ccatgcaagt tacctttaga atgaggtgtt taagcataaa
      481 tgtatgcatc tgggggccct gacatacgtt ttctctctgc gttttggctt gtggcaattc
      541 tctttctgtc cggtgtgagc agagcagaag accagggcgc cagagaccgc gcgtattccg
      601 tcgaagtcat gcagcggatt gccgctccag tgctcaccgc gatcagccag aacaagctga
      661 agcagaccat gcccgtcgaa ggccctcctg accgcgcgtc ttatacccat cttgaggccc
      721 ttgggcggct gctcgccggg atggccccct ggctggaact tggccctgat aatacttccg
      781 aaggaaagct gcgggcgcgc gacatccagc ttgcagtcca ggcaatttcg cacgctgtgg
      841 acccaaaatc gcccgactct atgaatttcg acaaaggcca gcagccgtta gtagacacgg
      901 cctttctcgc cctcgccctg ctgcgcgctc caaaacagct atggggaaat ctgggcgccg
      961 tggacaaagc caatgtggtc gctgctttga agaagacccg cgcgattcat ccctttgaaa
     1021 gcaactggct gctcttcagc gcgaccatcg aagccacgct gtggaagttc acaggcgagt
     1081 gcaaccagac cgccatcgag tacgccatca accgccatat ggagtggtac aaaggcgacg
     1141 gagtgtacgg cgacggcgcg catttccact gggactacta caacagctat gttatccacc
     1201 ccatgcttct ggaagtgctg cgcatctgcg cggaaaaaca gcatccgctg ggtaagctct
     1261 atctcgtgga gctggagcgg gcgcagcgtt acgcagtgat tcaggagcgc ctgatcgcgc
     1321 ccgacgcgag ctttcctgtg atggggcgtt cgagcgccta tcgcttcgga gcatttcaga
     1381 cgctttctga catcgcgctg atgcgtcagc ttcctccctc gcttgacccc ggcgcagtgc
     1441 gctccgccct gaccgccgtc atccgcagga tgatcgagac cccaggcaca tttgacgcgc
     1501 acggctggct ccggattggg gccgtaggcc accagccaca catcggcgag ggatatatct
     1561 caaccggaag cctgtatctg tgcacagccg gattgctgca cctgggtctg cctccagatg
     1621 acccgttctg gaccgccccg gcgcgtccgt ggacgcaaaa gcaaatatgg gcaggcgaag
     1681 acgtgcccgc cgaccatgcc atcgcgaatt agggcctgct acggagcaaa tcatagtcga
     1741 gggacaaaga gcctggcgac cgtcgagttt tacagctcct gaaagtggcc gcggcagcca
     1801 gcattcaccc cgaatccacg cacaccagta aaatagaaga cgatgagcac gaacctggta
     1861 acagccttaa cagtgatgct ggagaaggcc gcgcgtgagg ccggcctgac cgttctctct
     1921 gccgacgccg gcacggactt caatggccac cctaccgcca tcttcaagct ggcgctctcg
     1981 cccgacgctg ccccggagcg cacccttcag cttgagctga gcgatgcctt cgatttccag
     2041 aggcccgacc ttctgcccga gatgacctcg cacctgcgcg aagaggcaaa gcgtctgcgg
     2101 aatccgcggc ccaacactta cgttacgctg gccggactgc ctatccgctt cagcggtttc
     2161 ggatggccct tccaccgctc gacctccggc gcagacacct acatcgtcca cggcgtggtg
     2221 cacctggaag acggcaccaa ctcgccgctc cacgcaaagg tctcggccag tatgacggtc
     2281 acctttgctg aaatcgtgcc cgcgcccgag cagccctatg ccgagacgtt tatttacaac
     2341 gctatccgca agaccttcga ccagggccag cttgaactgc tcaagagcgg caaccgtcag
     2401 ccggttccgg tcactacgcg ctactacagc cgctggcaga agaagttctt cttcacggat
     2461 acggacgaca actcgcgcat ggaatttctg aagctcaaga cctactggct ctccggcgtg
     2521 ctcggcaacg gacagcccgt ctggatcgcc gacccgcgcg acgcgcagta tctgaa
//
LOCUS       DASRBV010000050        15382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_559416, whole genome shotgun sequence.
ACCESSION   DASRBV010000050 DASRBV010000000
VERSION     DASRBV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15382
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559416"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFA02_02120"
     CDS             complement(<1..537)
                     /locus_tag="VFA02_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZU08672.1"
                     /translation="MKTAISVSKEDYLKAILEAESEGQTVISATLAHWLEVSPPAVSM
                     ALRRLRRDGYVEVKADGIIRLTVKGRETAYRTALRHHLIERMLSEMFGMPWYEVHDEA
                     ERLEHAVSPAFEARLLEKLGEKGTCPHGNSVLPETPAQRARQGVQLLSGAKENAGYTV
                     CSLYERDPKLLRFLHQVGI"
     gene            complement(574..1389)
                     /locus_tag="VFA02_02125"
     CDS             complement(574..1389)
                     /locus_tag="VFA02_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08673.1"
                     /translation="MKASQGGNMRHALHLSGLAAAAFVCFLTFESAGQTPHSFIAIGS
                     YAQQPPLRAEDAPRFVLSAEMQGDLLMARQRYIAALDAYRQGPSNSPVLWNKMGIANH
                     HMFNLKAAENDYREAIKLDPKYPEAWNNLGAVYYGEKKYHDAEKAYRKAIKFSPKSAT
                     FYSNLGTAYVAQGKYKKGAEAYHTALSLDPNVFNGDPAAKVAEAGPSREMAYLNYLLA
                     KAYAETGRKDEALYYLRKALDEGFSDRKKLLEEKEFAGLRDNPEFRQMIAERR"
     gene            complement(1540..2823)
                     /locus_tag="VFA02_02130"
     CDS             complement(1540..2823)
                     /locus_tag="VFA02_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08674.1"
                     /translation="MSRMALLLCLFFAGPFLAWAQYQGPAAKDTSSAPTLRATAIFEW
                     TGDLAKPGAARLVPVAVWDGNEYQPGGLYLAQPEPLTLETGTLYELEKAGAPQGTFQI
                     NSAENLAGSWIGLGVIRPQPALAKPKSPMSKYPPQVVKEGAQSQQNAGPAKSSAPTPK
                     DDSGRPTLHRHDTADTTPPPVGDDSDRPTLHKRTSAANPDAPVTAAVAPDSGRPKLRY
                     GKPESPEGLVAPSKLEGFPPDLEQMAAVSDVKQSAPHSYAHIWYDPADEAKMKAAVEK
                     LAQDALAAENPATKYITAAGARKPARRKATAAPPLPVLTGEKFNALDLSWSSGVTIVF
                     SAQAAGQNGQTQYITLVAQPDFYGNPQIIFKQITRDDRLDVTPRMKLVDAVDTDGDGR
                     AELLFELRGVSGREFGIYRVMNRSVEQVFHTGPQL"
     gene            2888..3349
                     /gene="bcp"
                     /locus_tag="VFA02_02135"
     CDS             2888..3349
                     /gene="bcp"
                     /locus_tag="VFA02_02135"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZU08675.1"
                     /translation="MEVNDKVPDFALQDDQERTIHLSDYAGTPVVLFFYPRADTPGCT
                     IEACGFRDVFKKIQKAGAAVLGISRDTPKAQAKFKAKYDLPYPLLADVDEKVCNLFGV
                     LKDKNMYGKKVKGIERTTFLIGPDRTLLKIFPKVRPEGHAEEVLETLKALQ"
     gene            complement(3398..3967)
                     /locus_tag="VFA02_02140"
     CDS             complement(3398..3967)
                     /locus_tag="VFA02_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08676.1"
                     /translation="MHRFRYIFTRAAVVLVMTAMAIFAHAQDSASSGRIPAGTKLTVR
                     TSTELTSATAEAGQTWNGTLVNEVRSGGKVIAPAGSSVSGTIVDAKSSGRLKKPGVLS
                     LQITSVNGIAVNTDTLTRDGEGHTKSNVAKIGGGAAAGAILGGLIGGGKGAAIGTLAG
                     AGAGTAGAAATGKREAKIAPETALTFTVQ"
     gene            4001..4618
                     /locus_tag="VFA02_02145"
     CDS             4001..4618
                     /locus_tag="VFA02_02145"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZU08677.1"
                     /translation="MPTRNKVVSTVPRPFYEHSPEVVARKLLGKLIVRDYHGERLSGR
                     IVEAEAYFGLDDPAAHAFAGRTARNAVLFGPPGFAYVYFIYGMYYCLNVSCEPEGHAG
                     CVLIRALEPVQGLEMMAKLRKLPSAAKPRLLTSGPGRLCQALAITRAAHNGLDVTSTS
                     SILRIEDDGFQPGKISVTPRIGIRHAADRLARFVIEENKFVSRNL"
     gene            4683..5153
                     /locus_tag="VFA02_02150"
     CDS             4683..5153
                     /locus_tag="VFA02_02150"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU08678.1"
                     /translation="MKRLLLATATALFLTHVCATAQDAPPKQTSHQRMPLPKPTNLKV
                     LPKNISPEDLMKVMRGYSQALGVHCDFCHEVNEQTHQPNFASDAKPDKAIARTMIAMT
                     QTINQKYMSTINDPDATPEMKTVTCGTCHRGDTMPPPFQPKPEDHPKNASPQHP"
     gene            5482..5667
                     /locus_tag="VFA02_02155"
     CDS             5482..5667
                     /locus_tag="VFA02_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08679.1"
                     /translation="METGDAKRPLACIWTDADLRTPEAHMEEQEEPEPTAFLQILYTP
                     FALGYVADSLLLAVTVV"
     gene            5799..7259
                     /locus_tag="VFA02_02160"
     CDS             5799..7259
                     /locus_tag="VFA02_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU08680.1"
                     /translation="MREFAMIVCLILCPVLAGAQTSSLPNAPAPPERELLSQRSPSSE
                     AGVSPEERQAPDAPDVTMFPHSNTAPWWISGQTNIIFQAHPSFHSPYQGTNSLHGAGE
                     YKTSLLGTLFLGYQPHRNLRYNTDFLVDFESAGGRGLSQALGLAGFTNLDVVRNPNLS
                     SKPYLARGEIHQTIGLTDETTETDRGPLSLAAQVPVRRFEFRIGKMSMPDVFDINSIG
                     SDSHLQFTNWTIDNNGAWDYAADTRGYTVAAIAEYDDRDWSARYGIAAMPIVANGIDL
                     DWAFSRASGQNMEFELRRSFVPGRKGVTRILSYVNHAHMGSYREAVQAYLDGADPRPD
                     ITKHEHFSSVKYGFGWNNEQEITQDFRIFSRVGWNEGQHESFAYTEVDQTIAFGGDLQ
                     GIRWKRPVDKIGVAFVSNAIKRDHQNYLKYGGLGFLLGDGRLNYGRENIVESYYNWHT
                     WRGVFFALGLTHIDDPGYNRDRGPVWVPSVRMHVDY"
     gene            complement(7462..9747)
                     /locus_tag="VFA02_02165"
     CDS             complement(7462..9747)
                     /locus_tag="VFA02_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU08681.1"
                     /translation="MSTLIPEEKTHRTATFALIISVAVLHGMVLAFVPHPLLLSNVVQ
                     LASALFAVTVCILRWRSAEGPYFRWLWKQLCVAFLIWSVAQAYYLWYVIVRHRPTPYP
                     SIADFLWLSFSFPFLLAASRPYKLTVQGQRWTGHLDFAQACLSVLLLYGIIYVAPAGV
                     LDTVAYGIQALGLVLSCAIRFSTATVPEEYAFFWDLTLYGVIYGVIAVAGTLWQDYGS
                     PAGGITDLAWSFPLLAFCAIVYSFPARDRVSGFRTWKRNILPPHIHGVSSLGLALISL
                     AAGVILTAYRHVWGVAGLTVSCLLLVLRTAIREARLEQAQTQLQHAVLHDALTALPNR
                     SLLLSEIERVRSFSSSKFLLFLDLDRFKTINDSLGHKTGDKLLVHFAQVLRRTVWPEN
                     MVARLGGDEFVVLLERLDYGETAESVAERILSQARLPVLMDDRELYVTASIGIAPITK
                     EKSASELLRDADTAMYKAKALGKDRACTFNQSLVEMTMREMEMGSALRSSIEVGAVSV
                     AYQPVYSLKRAELAGFEALARWRHPLYGQVSPEEFVPLAERAGLITGLGRQVLRVACF
                     QIAAWNRHFKTRYSVSINVSGIELADHRFLPELEYVLRESHLDPALLALEVTESVLLN
                     DEHAAREVLGKAHDLGVSIYLDDFGTGYSSLKYVLQFPFDVIKIDKGFVHNVERDGKR
                     AELLKAIVYLSRNLHKRTIAEGIETPDQMRFLTSIDCEFGQGFLLSRPLLPDQVFYLL
                     QSRTSESMLQAAPLLLKPEHI"
     gene            complement(9920..10966)
                     /locus_tag="VFA02_02170"
     CDS             complement(9920..10966)
                     /locus_tag="VFA02_02170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZU08682.1"
                     /translation="MRFLFAMLMIACALPGFAQAAASSNGLPSDPKAQKAYSEALDWL
                     NHRDYAAAVSSFKKADKQDGGHCIACQNKIIELGEKTGDYKSAEFAAQEMIDEAQAPV
                     DQAEAHVRKGVLLLREGVVKNKEDMFAEADREFKAAEAADPKHMQAYYGDGFALAHLK
                     RDDEARDQFQQFVALKKTGVDSVRASRYAEHPELARARMAPPFATVTADGKRVSLDDF
                     TGKVVLIDFWATWCGPCRQALPHIQKIAEKFSGEPLVIISVSLDSDEQKWRSFIAQNH
                     MTWIQVRDGGFTGPLSRKFGVNAIPHTFTIDTDGVLQDEHIGDGSMEGKLKKLCAQAR
                     QQQEQPKQASAEMR"
     gene            complement(11074..11289)
                     /locus_tag="VFA02_02175"
     CDS             complement(11074..11289)
                     /locus_tag="VFA02_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08683.1"
                     /translation="MIPAITTTHAVPQKLLPARIGMVRSRIILCHHCCQVLGCAETPG
                     DRERLRAAHVCKEKLMDQQPGAAVPFN"
     gene            11575..12522
                     /locus_tag="VFA02_02180"
     CDS             11575..12522
                     /locus_tag="VFA02_02180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU08684.1"
                     /translation="MASDPQNRPSILLADPDPESVEVLVPLLQTWNYEIETVRDGVEG
                     FKRLLAPDAPAIAILDDNLPHMRGIEIIAEIKRRSRPYSSWLMLMGQAPDGETVSLAS
                     AAGVDDFLLKPVSETDLLVRLRTAERVQHLYREMQEQTQALHFRATHDPLTGLLNRES
                     TMRQLFQETDRAQRMRTPLCIMLLDMDDFSEINQEYGFSAGDAVLKELAQRLKRYMRS
                     YDVLGRCGNDDFLIGLPGCMVEDAIALAQRLQKSVLAKPCHIHRDVIPVTVSIGIASS
                     RGRLPLVVLREAEDALMQARMAGKGGIRFSGPAPQSNRL"
     gene            complement(12519..15290)
                     /locus_tag="VFA02_02185"
     CDS             complement(12519..15290)
                     /locus_tag="VFA02_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZU08685.1"
                     /translation="MKRNFLLLLLCSACAAQTIPLPTSKQIVAPVPGSPQRLNSLPMM
                     MAWSPDHRYLAMVNAGYGTVESGYAQSIAVLDTSTGKLMDFPEERAVLGSAQTLYNGL
                     VFSADGSRLYASLDSLAQPGTGNQIAVYRFDGATLAPEHTIPVPLQKLAPGHTQTRVS
                     KDVPEGMAVPAPTGLAVVKGDSGAEEILVADEFSDDVLLMDAASGNVLHRFDLADHPT
                     VPSEYPIALIATKNQRRAFVALWNGSAVAELDLTSGKVVQKLPLLPPDNPISPGSHPA
                     ALVFSPDERTLYVALANRDAVAAVSVRGQKMQLAGMFNTQLPGQHYFGAIPDALALSG
                     DGRTLYVANGGSDAVAVFSAVNLKTGAAVEAKAFIPTEWFPTALAIKGNHLFIATGKG
                     QSTVPNAAPQPQPEHPLPHSRNMRRAHTYIATMLHGSLADVDLNSAQNELPRLTSEVL
                     ASNMMQAASQHLKFLGGRNPIHHVIYIIKENRTYDQIFGDLGVGNGDPSLTMYGKSIT
                     PNLHKLALQFGVLDNFYDSGEVSGDGHVWSNAAISSDYTEKTWQQAYRGSARVYDFEG
                     IVEEGNPYKEGIPDVNEPASSYIWTDLAKHGKTLYHFGEYIATTFCEEHREQPKEKSP
                     LAGTPEPEPERCPVAAIHKGDSIPANYGGGVSPYPWAIPLIAKNVATKPELEGHYDPL
                     YPDFELAFPDQLRVEEFLTHFRKWVADRQQGHDTMPDFVMLRLPNDHTAGTRPGMPTP
                     RASVADNDLAVGRAVEAVSHSPYWDDTAFFILEDDAQDGADHVDAHRSTALIVSKYAP
                     RNPQPLVDSTFYTTVSFLRTMEDLLGVPPMNNNDALAPLVSSVFTGAGDQPPFTADAS
                     NRDNGLIYTANTPKSPGAKESGKMDFTHEDRADPVKLNVILWRDAMGDKPVPWMVLHP
                     HTGRKDDDD"
BASE COUNT         3407 a         4290 c         4248 g         3437 t
ORIGIN      
        1 aatgcccacc tggtgcagaa agcgcagcag cttcggatca cgctcataca ggctgcagac
       61 ggtgtagccg gcgttctctt ttgctcccga aagcaactgt actccctgcc ttgcgcgctg
      121 tgccggtgtc tccggcagaa cagaatttcc atggggacag gtgccttttt cccccaattt
      181 ttcaaggaga cgggcttcaa aagcgggtga gacggcgtgc tccaggcgtt cggcttcatc
      241 atgcacttca taccagggca ttccgaacat ctcagataac atgcgctcta taagatggtg
      301 gcgcagtgct gtgcgatagg cggtttctct gccctttacc gtaagacgga tgatgccgtc
      361 agctttgacc tcaacatatc catcgcgcct gaggcggcgg agcgccattg agaccgcggg
      421 gggagaaacc tcaagccaat gggccagtgt agcggaaatg accgtctgac cttcgctttc
      481 agcttcaagg atggccttga gatagtcctc tttagaaacg ctgatcgcag tcttcaccgc
      541 aattaggata caactactgg ctgttatgag accctagcgc cgctcggcta tcatttgcct
      601 gaactcagga ttgtcgcgta gtccggcaaa ttccttttct tctagcagct tcttgcggtc
      661 actgaaccct tcgtcgagcg ctttacgcaa ataataaagc gcctcgtctt ttcttccggt
      721 ttcggcatag gcctttgcga gaagatagtt cagataagcc atttcccggg atgggccagc
      781 ctcggcaact ttggcggcgg gatctccatt aaagacattg ggatccagac taagcgcagt
      841 gtggtatgcc tctgcgcctt tcttgtattt gccctgggcc acataggctg ttcccagatt
      901 gctgtagaag gtggccgatt tcggcgaaaa ctttatggcc ttcctgtatg ccttctcggc
      961 atcatgatat ttcttttctc cgtaatacac cgcaccaaga ttgttccatg cctccggata
     1021 ttttggatcg agcttgatcg cttcccggta atcgttctca gcggccttca ggttgaacat
     1081 gtgatggttg gcaataccca tcttgttcca gagcacaggt gaattcgatg ggccttgccg
     1141 ataggcgtct agagcagcaa tatacctctg gcgcgccatc agcaggtcgc cctgcatttc
     1201 cgcgctcagg acgaagcggg gcgcgtcttc tgctctgagc ggaggttgtt gcgcgtaact
     1261 gcctatcgca atgaacgaat ggggtgtctg tcctgcggac tcaaacgtga gaaaacaaac
     1321 aaacgctgcg gcagcaagac ctgagaggtg tagagcatgg cgcatattgc ctccttgcga
     1381 ggctttcatc ctgcatctgc cgggcgcggg ggatgagctg tgtccggaca gacccagcct
     1441 ttggccccta attgtaactc tctagataaa gaacaagcga taaaagcggt gttgactgga
     1501 taaaaagcgc caaaatggtc atttggcgcg gtcctggcgt tacaactgcg ggccggtgtg
     1561 aaagacctgt tcgacactcc ggttcatgac gcggtaaata ccgaattccc tgcccgaaac
     1621 gcctcgcagc tcaaagagca actcggcgcg gccgtcgcca tccgtgtcca cagcatctac
     1681 cagcttcatg cgtggagtaa cgtccaggcg atcatcccgg gtgatttgtt tgaagatgat
     1741 ctgtggattg ccataaaaat ccggctgcgc aactagcgtg atgtattgcg tctgtccatt
     1801 ttgaccagcg gcttgggcgc tgaagacgat ggtcacgccg ctgctccagg aaagatcgag
     1861 cgcgttgaat ttctcaccgg tcaggacagg caagggaggc gccgcagtgg ccttgcgtcg
     1921 ggccggtttt cttgcgcctg ccgcagttat atattttgtc gccggatttt ccgcagcgag
     1981 cgcatcctgg gccagttttt caaccgcagc cttcatcttt gcctcatccg ccgggtcata
     2041 ccaaatatgc gcgtaagaat ggggcgcgga ctgctttaca tcagaaacag cagccatctg
     2101 ttccaggtct ggaggaaatc cctcaagttt ggaaggcgct actaaccctt ccggggactc
     2161 aggcttgcca tagcggagtt tcggtcggcc actatcgggg gcaaccgctg ctgtcacagg
     2221 cgcgtccgga ttggccgcag atgtccgctt atgtaatgtg gggcggtcag aatcatcgcc
     2281 caccggaggc ggagtggtat cggccgtgtc atgacgatga agcgtgggac ggccggaatc
     2341 atctttggga gtgggcgcgc tgctcttcgc cggccctgca ttctgctgcg actgcgcccc
     2401 ttctttcacc acctgcgggg gatatttcga cataggcgac tttggtttgg ccaatgccgg
     2461 ttgtggcctg atcacaccca gaccaatcca tgaacctgca aggttctcgg cgctgttgat
     2521 ctgaaatgtt ccctgcgggg ctccggcctt ctctagttcg tatagagtgc cggtctccag
     2581 cgtcaagggc tcgggctggg ccagataaag acctcccggc tgatactcat tgccgtccca
     2641 gactgctacg ggaaccagac gcgccgcccc gggttttgcc agatcgcctg tccactcaaa
     2701 aatggcggtg gcgcgcagcg ttggcgccga gcttgtgtct tttgccgcag gcccctgata
     2761 ctgcgcccag gccagaaaag ggcctgcgaa gaacaggcag agtagcaatg ccatccgtga
     2821 catacgatgg cgttctgcga aacaatcgga gtggcccata catctatttt ggcggaagga
     2881 atgattcatg gaagtcaacg acaaagttcc tgattttgct ctccaggatg accaggagag
     2941 aaccatccat ctttcagatt acgccggaac gccggtcgtg ttgttctttt atccccgcgc
     3001 tgatactccg ggctgcacga ttgaggcctg cggcttccgc gatgtcttca aaaaaatcca
     3061 gaaggcggga gcggcagtgc ttggcatctc gcgcgacacg ccgaaggccc aggcaaaatt
     3121 caaggcaaag tatgacctgc cgtatcctct ccttgctgat gtagacgaaa aagtgtgcaa
     3181 tctgtttggc gtgctcaagg acaagaatat gtacggcaaa aaagtaaaag gcatcgagcg
     3241 gacgacattt ctgattggcc cagaccgcac cctgctgaag atctttccta aagtcagacc
     3301 ggagggccat gccgaagagg tcctcgaaac cctgaaggca ctgcaataaa gaaggcggca
     3361 ccatggccgc cttcacgaat gattgtccgc cagtccctta ctgcactgta aatgtcaggg
     3421 ccgtttccgg ggcgattttt gcttctcttt ttccagtagc tgctgcgccg gccgtgcctg
     3481 ctcctgcgcc tgccagcgtt ccgatggctg ctcccttgcc gccgccaatg agtccaccta
     3541 gaattgcgcc cgccgcggca ccgccaccaa ttttcgctac attacttttg gtatggcctt
     3601 caccatcgcg tgtaagagtg tcggtattca ctgcaatgcc attgactgaa gtaatttgca
     3661 gcgagagcac ccccggcttc ttcagccttc ccgatgattt cgcgtctacg atggtcccgc
     3721 tcacgctgga tcctgccgga gcaatgacct ttccgccaga acggacctca tttaccaaag
     3781 tgccgttcca tgtttgtcct gcctctgcgg tggctgaagt aagctcggtg ctggtgcgaa
     3841 cggtcaactt tgttcctgcc ggaatccggc ctgagctggc tgaatcctga gcatgagcaa
     3901 atatggccat cgccgtcatg acaagaacaa cagcggcccg tgtaaaaatg tatctgaaac
     3961 gatgcatctt tctttctcct ggaaaccttt ttaatcggat atgcctactc ggaataaggt
     4021 tgtctccacc gtgccgcgcc cgttctatga gcattctcct gaggttgtgg cccgcaagct
     4081 attgggaaag ctgattgtgc gcgattacca cggagaacgg ctctcgggcc gcatcgtgga
     4141 agctgaagcg tattttggtc tggatgatcc ggcggcccat gcctttgccg gacggacggc
     4201 ccgcaatgct gtgctgttcg gccctcccgg gtttgcctat gtttatttca tttacggcat
     4261 gtattactgt ctgaatgtct cctgcgagcc ggaagggcat gctggttgcg tcctgattcg
     4321 cgcgcttgag ccggtacagg gactggaaat gatggcgaaa ctgcgcaagc tgccatctgc
     4381 cgcaaaaccg cggctgctca cttccggtcc aggacgcctc tgtcaggcct tggccattac
     4441 tcgcgccgcg cataacgggc tggatgtaac cagcacctct tctattttgc gtattgaaga
     4501 cgatggtttc caaccgggga agatttctgt cactccacgc atcggaatcc ggcacgctgc
     4561 tgaccgcctc gcgcgctttg tcatagaaga aaacaaattt gtttcgcgaa atttgtaaac
     4621 tttccacata cttgtacgta tactttgcag caccagaaga cactgttcca cagggagacc
     4681 gaatgaagcg ccttcttctc gccaccgcca cagctttgtt tctgacccat gtctgcgcca
     4741 cggcccagga cgcgcctcca aaacagactt cgcatcagcg gatgcctctg ccgaaaccaa
     4801 ccaatctaaa ggtgctcccg aaaaacatct caccagagga cctgatgaaa gtcatgcgcg
     4861 gctactccca ggcgcttgga gtgcattgcg atttctgcca tgaggtcaac gagcagacac
     4921 accagccgaa tttcgcctct gacgcgaagc cggacaaagc cattgctcgc accatgatcg
     4981 ccatgacgca gacgatcaat cagaaataca tgtccaccat caacgatcct gacgctactc
     5041 cggagatgaa gaccgtcacc tgtggaacat gccatcgcgg tgacacgatg cctccgccgt
     5101 tccagccaaa accggaagac caccctaaga acgcgtcgcc acagcacccg tagtgtgatg
     5161 gcccgcaggt ccacatgtgt ttctgttcgt gacctgccaa aatttttcgc gcacttctat
     5221 atatcctacc cgggtatgat ataaaagaag aggtgcggga aacactccgc cagagcctga
     5281 aacctatgaa tactgaaggc cattcagacc atactcctgc gcagtgaagg gactgcaccc
     5341 tgctccctca cggcgcgcga cgtccacgtg agtgagcagt cttctgccca cctccatttc
     5401 acaaacagaa aggaggggcc cgtggtcctg aacacctgta ctttttttga aacgcagcgc
     5461 agtcagctgt cctgcgtctg gatggaaacc ggagacgcga agcgtccgct tgcctgcatc
     5521 tggacagatg cggatttgcg aacgccggag gcccacatgg aggaacagga ggaacccgag
     5581 cctacggcat ttttacagat tctgtacact ccatttgcac tgggctatgt cgccgactcc
     5641 ctgttgctcg cggtgactgt agtttagtgg catcagccag actatctgtg aaaatgctat
     5701 gtcctgctgt tgtgcgctta acacaattgc aacctttgta ttgaaacatc agaaaagctg
     5761 actttttctg gttgatttga tttatctcgg agaccttttt gcgcgaattt gccatgattg
     5821 tctgtctgat attgtgcccc gtattggccg gggcacagac ctcttccctg ccgaatgcgc
     5881 cggcgccacc tgagcgggag ttgctctcgc agcgcagtcc ttcgagcgag gccggggtct
     5941 caccagaaga gcgtcaggcg cccgatgcgc cagacgtgac gatgttcccg cacagcaaca
     6001 cggccccgtg gtggatctcc ggccagacga acattatctt tcaggcgcac ccctcctttc
     6061 actcgccgta tcagggcacg aacagccttc acggagcagg cgaatacaaa acttcgctgc
     6121 tgggcacact ttttctcggc taccaaccgc accgtaatct tcgttacaac accgatttcc
     6181 tcgttgactt cgagagtgcc ggaggacgcg gcctgagcca ggcactgggt ctggccggat
     6241 tcacgaacct tgatgtggtc cgcaatccga atctgagttc caaaccctat ctggcgcgcg
     6301 gagaaatcca tcagaccatc ggtcttacgg acgagaccac tgagaccgac cgtggcccac
     6361 tctcactggc agcccaggtg ccggtgcgcc gcttcgagtt tcgcatcggc aaaatgagta
     6421 tgccggatgt cttcgacatc aactccattg gaagcgacag ccatctgcaa ttcaccaact
     6481 ggaccattga taataacggc gcctgggact acgccgccga tacacgcgga tataccgtgg
     6541 ccgcgattgc cgaatacgat gaccgcgact ggagcgcgcg ttatggaatc gcagcgatgc
     6601 cgattgtggc caacggaatt gatctggact gggcgttttc gcgcgccagc ggtcagaaca
     6661 tggagttcga gctgcggcgg tcgtttgtac ccggacgcaa gggcgtcaca cgcattctca
     6721 gctatgtgaa ccatgcgcat atggggagtt accgcgaagc tgtgcaggct tacctggatg
     6781 gcgcagaccc tcggcccgat atcacgaagc atgagcattt cagctcagtg aagtatggct
     6841 ttggctggaa caacgagcag gagatcacgc aggacttccg catcttcagc cgcgttggat
     6901 ggaatgaagg ccagcatgag tcctttgcat acacggaagt agaccagacc atcgcctttg
     6961 gaggggatct tcagggcatt cgctggaagc gtccggtgga taagatcggt gttgcttttg
     7021 tttctaatgc cattaaacgc gaccatcaga attatctgaa atacggcggc ctcggctttc
     7081 tgcttggcga tgggcggctc aattatgggc gcgagaacat tgtagagagc tactacaact
     7141 ggcatacctg gcgcggcgtg ttcttcgcgc tcgggctgac acatattgat gaccccggat
     7201 acaaccgcga ccgcggcccg gtctgggtac cctctgtacg catgcatgtg gattattgaa
     7261 agaacggcaa agtctgccat gttctattca tcctgcccac gcgcaacgtc agccctactg
     7321 aagaactcgc cagtatgttg atgctatagc aatttcacag atagtctgaa ccatgcccca
     7381 aaaactacag tcgccgcgac cagcagggag tcggcgacac tgcatagtgc aaatggaggg
     7441 taaagaatct gtgaaaatgc cttaaatgtg ctcaggctta agtaggagag gcgctgcctg
     7501 gagcatactc tcgcttgtcc tggactgcag cagatagaac acctggtctg gaagcagcgg
     7561 ccgggagagc aggaagccct ggccaaattc gcagtcaatg ctcgtcagaa agcgcatctg
     7621 gtcgggggtt tcgattccct ccgcgatggt tctcttatga aggtttcgcg aaagatacac
     7681 aatggcttta agaagctcgg cccgtttccc gtcccgctcc acgttatgaa caaagccttt
     7741 atctatcttg atgacgtcga aaggaaactg aagtacgtac ttgagcgaag agtacccagt
     7801 accaaaatcg tccagataaa ttgagacgcc gagatcatgg gccttgccca gcacttctct
     7861 ggcagcatgc tcgtcattga gcagcaccga ttcggtgact tccagcgcaa gcaatgccgg
     7921 atccagatga ctctcccgca aaacatattc cagctccggc aaaaacctgt ggtccgcgag
     7981 ctcaattcct gagacattta tgcttacgct gtatctggtt ttaaagtgac ggttccatgc
     8041 ggcaatctgg aagcaggcga cccgcagcac ctgccgtcca agtcccgtaa tcagcccggc
     8101 gcgctcggcc agtggcacaa actcttcggg tgaaacctgc ccgtacaggg ggtgccgcca
     8161 tcgcgccagg gcctcaaacc cagccagttc tgcccgtttg agcgaataga ccggctggta
     8221 ggcaaccgaa acagcgccca cttcaatgga agagcgcagc gcggagccca tttccatttc
     8281 gcgcatcgtc atttcgacaa gtgactggtt gaaggtgcag gcgcggtctt tgcccaaggc
     8341 ctttgccttg tacatggctg tatcggcatc gcgaaggagt tcgctggcgc tcttttcctt
     8401 ggtaatggga gcaattccta tgctggccgt cacatacagc tctctgtcgt ccatcagcac
     8461 aggcaggcgg gcctgagaca aaattcgttc cgctacactt tcggcggttt cgccataatc
     8521 gagcctttcc agcaaaacta caaattcatc tccgccgagc ctggcaacca tgttttcagg
     8581 ccagacagtc cttcgcagga cctgcgcgaa gtgcaccagc agcttgtcac cagttttgtg
     8641 tcccagactg tcattgatcg ttttgaaacg gtctaagtca aggaacaaca ggaatttact
     8701 ggaagaaaaa gagcggaccc tctcaatttc tgaaagcagg agactgcggt tcggcaacgc
     8761 tgtgagcgca tcgtgcagga ccgcgtgttg aagctgtgtc tgtgcctgct ccagacgcgc
     8821 ttccctgatt gcagttctta ataccagcaa caggcaggag acggtgagac cagctactcc
     8881 ccacacatgg cggtatgcgg tcaggatgac accggcggcg agtgaaatca gggccagccc
     8941 cagagagctt acgccatgaa tgtgcggcgg caaaatgttt cttttccatg tgcgaaatcc
     9001 gctgacccgg tcccgggctg gaaagctata aacaattgcg cagaacgcca gtaaaggaaa
     9061 actccacgcg aggtctgtga tgccccctgc cggagaccca tagtcctgcc acagcgttcc
     9121 cgccacggcg atcacaccat agatgactcc atacaaagtc aggtcccaga aaaacgcata
     9181 ctcttcaggg actgtcgcag tagagaaacg gatggcgcat gacaagacca acccgagggc
     9241 ctgaatacca taggcaacgg tatccaggac cccggcaggt gcaacataaa tgattccgta
     9301 aagtagcagt acagacagac acgcctgggc gaaatccaga tgtcccgtcc agcgctgtcc
     9361 ctggactgtc aatttgtagg gccgcgaggc agccagcaga aatgggaaag aaaatgacag
     9421 ccacagaaaa tcggcaatcg agggataggg tgtgggccgg tgccggacaa tcacgtacca
     9481 caggtaatac gcctgagcca cgctccatat caggaaagcc acacaaagct gcttccaaag
     9541 ccagcgaaaa taagggcctt cagccgagcg ccatcgcaat atgcacacgg tgaccgcaaa
     9601 cagagcagat gctagctgca ccacattgga cagcaagagc ggatgaggaa caaaggccaa
     9661 caccatgcca tgcaggaccg ctacgcttat gatgagggcg aaggtggccg tccggtgcgt
     9721 tttttcttcc ggaatgagcg tgctcaaggc tataccaatc aagaaaaaca ggcttgtaaa
     9781 cggtctcagg tttctgtaat agtaatcggc tttttagaaa attttgagaa aaatttctta
     9841 atagggagca tctggcccat acagggccat tcccgatgta agcgcgggag aggccccagg
     9901 gagtcaagcc catgcttttt caacgcattt cagcgctcgc ctgcttaggc tgttcctgct
     9961 gctggcgtgc ctgggcgcac agttttttca gttttccctc catggaacca tcaccaatgt
    10021 gttcgtcctg cagcacaccg tcggtatcaa tcgtaaaggt atgcggaatc gcgttgacac
    10081 cgaacttgcg cgagagcggc ccggtaaacc cgccgtcgcg tacctggatc catgtcatgt
    10141 ggttctgggc gatgaagctg cgccacttct gctcgtcgga atcgaggctc acactgataa
    10201 tgaccagcgg ctcgccggaa aatttctccg cgatcttctg aatatgcggg agtgcctgac
    10261 ggcatggccc gcaccaggta gcccagaagt ctatcagcac gacttttcca gtgaaatcat
    10321 ccagagaaac gcgcttcccg tcggcggtca ccgttgcgaa cggaggtgcc atgcgcgcac
    10381 gcgccagctc gggatgctcg gcatagcggg aagcccgcac gctgtccacg cctgttttct
    10441 taagtgcgac aaattgctga aattggtctc tggcctcatc atcccgcttc aggtgcgcca
    10501 gggcgaagcc gtctccatag tacgcctgca tatgttttgg gtcggcggct tcagcggcct
    10561 taaattccct gtcagcttcg gcaaacatat cttctttgtt tttcaccacg ccttcgcgca
    10621 gcagcaacac gccttttcgg acatgggcct cggcttggtc cacaggggcc tgcgcctcgt
    10681 caatcatctc ctgcgccgcg aactccgcgg acttatagtc gccggtcttt tcgccgagtt
    10741 cgatgatttt gttctggcag gcaatgcagt ggcctccgtc ctgtttgtcg gcctttttga
    10801 aggaacttac agcagcggca taatcacgat ggttcaacca gtccaatgcc tcggagtagg
    10861 ccttctgcgc cttcgggtcc gaaggcagac cattggatga agcggcagct tgcgcaaaac
    10921 caggcagagc acatgctatc atcagcatgg caaaaagaaa tctcatcaac acagttcccc
    10981 tctgacggaa aaaccatcat gccatttcac agacgggctg aaaagagaaa agcgcgcccg
    11041 aagacgcgct ttctcagggg aggaattcca gctctagtta aacgggacgg cagcccccgg
    11101 ttgctggtcc atcaatttct ctttgcacac atgggccgcc cggagacgtt cacgatctcc
    11161 cggagtttcc gcgcatccca acacctggca acagtggtgg caaaggatga tgcggctgcg
    11221 gaccatgcca atgcgggcag gcagcaattt ttgcggaacg gcgtgcgttg ttgtgatggc
    11281 gggaatcatg gctcactcca acgttaagga ttagaggtgt tgtggaccgg cgcagcttta
    11341 acaccgaagg gaaaacttgc cagaatttct ctggttacta acgatctgga tgccaatagt
    11401 aacctgtcag gttggccgct tgtgtaaatt tttgcgcatt tgacgccaaa atggaggaaa
    11461 aatttacaaa attcgcccaa tttttcttta tctggtgaaa acggctgcat tgcttccata
    11521 cacttcatgg aattttgaaa attctccgat atgcggctat gagacaaccc tctcatggct
    11581 tctgatccgc aaaaccgtcc atctattctg ctcgcagacc ccgatccgga aagcgtcgag
    11641 gtcctggttc cgctgcttca gacatggaat tacgagatag aaaccgttag ggatggcgtg
    11701 gaaggcttta agcgtctgct ggctcccgat gccccggcga tagccattct ggacgataac
    11761 ttgccccata tgagagggat agaaattatt gccgaaatca agcggcggtc gcgtccttat
    11821 tcatcgtggc tgatgctgat gggtcaggcg ccggatggag agaccgtcag cctggcaagc
    11881 gccgccgggg tggatgattt tcttctgaaa ccggtgagcg aaaccgacct tctggtgaga
    11941 ttgaggacag ccgaacgtgt ccagcacctc taccgggaaa tgcaggaaca gacgcaggcg
    12001 ctgcatttcc gggccaccca tgatccgctg accgggcttt taaaccggga atccaccatg
    12061 cggcagctct ttcaggaaac cgaccgtgcc cagcggatgc gcaccccgct ctgcatcatg
    12121 ctgctcgata tggacgactt ttcagaaatc aatcaggaat atggtttttc cgcgggagat
    12181 gccgtcctga aagaactggc acaacggctt aaacgctata tgcgcagcta tgacgttctg
    12241 ggcaggtgcg gaaatgatga cttcctgatt gggctgcccg gatgtatggt cgaagatgcc
    12301 atcgcgctgg cacagcggct gcaaaagtct gtgctggcaa agccttgcca tattcaccgc
    12361 gatgtaattc ctgtgaccgt gagcataggc attgccagca gccgtggacg gctgccgctg
    12421 gttgtgctgc gcgaagccga agacgcgctg atgcaggcca gaatggccgg aaaaggcggc
    12481 atccgcttct ctggccccgc cccgcagtca aaccgccttt agtcatcatc atccttgcgc
    12541 ccggtatgcg gatgcagcac catccacggg acaggtttgt cacccatggc atcgcgccag
    12601 aggatgacat tgagttttac cgggtcagca cggtcttcat gggtgaagtc cattttgccg
    12661 gattctttcg ctcctggaga cttgggtgtg ttggccgtgt agatcagacc gttatcgcga
    12721 ttggaagcat ccgcggtaaa tggaggctgg tctcctgctc cggtgaaaac ggacgatacc
    12781 agcggcgcaa gtgcatcgtt attgttcata ggcggaacgc ccagaaggtc ttccatggtg
    12841 cgcaggaagc tcactgtggt gtagaaagtt gaatccacca gcggctgcgg attgcgcggc
    12901 gcatatttgc tgacgatgag cgcggtgctg cggtgcgcgt ccacatggtc ggcaccgtcc
    12961 tgcgcgtcat cttccaggat gaaaaaggcg gtgtcgtccc agtacgggct gtgcgaaacg
    13021 gcttccaccg cgcgacctac tgcgagatca ttgtcggcca ctgaggcgcg cggcgtgggc
    13081 attccgggac gggtcccggc ggtgtggtca ttgggcaggc gcagcatgac aaagtcaggc
    13141 atggtgtcat gaccctgttg gcggtctgcg acccacttgc ggaagtgcgt caggaactcc
    13201 tcgacacgaa gctggtccgg gaaggccagc tcaaaatcgg gataaagcgg gtcgtagtgg
    13261 ccttccagtt ccggcttggt ggccacgttt ttggcaatca gtggaatggc ccacggatac
    13321 gggctgacgc ctccgccata attggcagga atggagtcgc ctttgtgaat ggcagcaacg
    13381 gggcaacgct ccggttcggg ttccggtgtt ccggcaagcg gagacttctc cttcggctgt
    13441 tcccggtgct cctcgcaaaa tgtcgtcgcg atgtattcgc cgaaatgata cagcgtcttg
    13501 ccgtgctttg cgaggtcggt ccagatgtaa ctgctggccg gctcatttac atccggaatg
    13561 ccttccttgt acggattgcc ttcctcgacg atgccttcaa agtcatagac gcgcgcgctg
    13621 ccacgatagg cctgttgcca ggtcttctcg gtgtaatcac tgctaatggc ggcattggac
    13681 cagacatgtc cgtcgccgga gacttcgccg gaatcataaa aattgtccag cactccgaac
    13741 tgcaatgcca gcttgtgcag attgggtgta atgctctttc catacatcgt cagtgaagga
    13801 tcgccattgc ccacgccgag gtcgccgaaa atctggtcgt atgtgcgatt ttctttaatg
    13861 atgtagatga cgtgatggat ggggtttctc ccgcccagga acttcaggtg ctgcgaggcg
    13921 gcctgcatca tgttgctggc gaggacttct gacgtgaggc ggggtagttc attctgtgcg
    13981 ctgttcaggt ccacatctgc cagtgagccg tgcagcatgg tcgcaatata tgtatgtgcg
    14041 cggcgcatat tgcggctgtg gggcaacgga tgttccggct ggggttgcgg agctgcattc
    14101 ggcacggtgc tctgcccctt tcctgttgca atgaacaggt gattgccttt gatggcaagc
    14161 gcggttggga accattccgt aggaataaat gctttggctt ctactgccgc gccggttttc
    14221 agattgacag cagagaaaac cgcgacagca tcagagccgc catttgcgac atagagcgtt
    14281 ctgccgtcgc ctgaaagcgc cagcgcatcc ggaatcgccc caaaataatg ctgcccgggc
    14341 agttgcgtat tgaacatgcc ggcaagctgc attttctggc cacgtacact gacggctgcc
    14401 accgcgtccc ggttggcgag tgcaacatac agcgtgcgtt catcgggaga aaagaccaat
    14461 gcggcgggat gtgaaccggg agagataggg ttgtccggcg gcagcaaggg cagcttctgc
    14521 actacttttc ccgatgtaag atccagttcc gctacggccg agccgttcca aagggctaca
    14581 aaggcgcggc gttggttctt tgtcgcaatc aaggcaatcg ggtactcgga tgggacggtt
    14641 ggatggtccg ccagatcaaa ccggtgaagg acgtttcccg atgcggcatc cataagcaaa
    14701 acatcatcag aaaactcatc ggcaaccaga atctcctctg cgccggagtc cccttttacc
    14761 accgccagcc cggttggagc aggaacggcc attccttcag gaacatcttt gctaacacgg
    14821 gtctgcgtat gcccgggagc gagtttctgc aacggaacag gaatggtgtg ctctggtgca
    14881 agtgtcgcgc cgtcgaaacg ataaacagcg atctgattgc ctgtaccagg ctgcgcgagg
    14941 gaatccagac tggcataaag gcgcgagcca tccgcgctga agaccagtcc gttatagagc
    15001 gtctgcgcag agcccagtac agcgcgctct tcaggaaaat ccatcagctt tccggtgctc
    15061 gtatccagca cagcaatgga ctgggcgtaa cccgactcga ccgtaccata gccggcattc
    15121 accatcgcca gatagcggtg gtccggggac caggccatca tcatgggcag gctgttcagg
    15181 cgctgaggcg aacctggcac cggcgcgaca atctgtttgc tggtgggaag aggaatggtt
    15241 tgtgcggcac aggcagaaca caacagaagc agcagaaaat ttcgtttcat ggcacgatag
    15301 caaactatca cgacgacatt acaaaagtct tacatgcacc atgccggcac gctgatacgt
    15361 tggaatgagg aagatatttc ct
//
LOCUS       DASRBV010000051         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_713728, whole genome shotgun sequence.
ACCESSION   DASRBV010000051 DASRBV010000000
VERSION     DASRBV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713728"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFA02_02190"
     CDS             <1..428
                     /locus_tag="VFA02_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZU08686.1"
                     /translation="GMNLGGTWRHEDVAVSMVEAKHSSGIVDGDLKIYGGEPCGFVLT
                     IENGPVLYHAGDTAVFSDMQLVRDLYKPQLAMLPIGDHYTMGPKAAALAAKFLGVKTV
                     LPIHYGTFPVLTGTPEELRKHLEGSGIEVKAISPGESIR"
     gene            614..1654
                     /gene="tdh"
                     /locus_tag="VFA02_02195"
     CDS             614..1654
                     /gene="tdh"
                     /locus_tag="VFA02_02195"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZU08687.1"
                     /translation="MKALVKSRPERGLWIEDVPEPQTGINDVKIRVLMTGICGTDLHI
                     YEWDAWAQKTIPVPLVIGHEFVGQIVEVGSNVADFQPGDLVSGEGHVVCGRCRNCLGG
                     RRHLCAHTQGVGVNRAGAFAEYVVLPMTNIWRHAPGIPLDVAAIFDPLGNAVHTAAAF
                     PVLGEDVLVTGAGPIGIMAAAVAKHAAARHVVITDLNPYRLELARMAAGVTEAINPRE
                     TTLEQVQKQLGMQEGFDVGLEMSGNPHAFRDMLANLSHGAKIAMLGIPSPSEMAIDWA
                     RVIFNQYTIRGIYGREMYETWYKMTVMLQSGLDISKVITHRFAWNEFEQGFAAMRSGN
                     SGKVLLDWQHLS"
     gene            complement(1680..1769)
                     /locus_tag="VFA02_02200"
     tRNA            complement(1680..1769)
                     /locus_tag="VFA02_02200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1733..1735),aa:Ser,seq:gga)
     gene            1896..3263
                     /locus_tag="VFA02_02205"
     CDS             1896..3263
                     /locus_tag="VFA02_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZU08688.1"
                     /translation="MRVQPQDKNITRELRDLLALAIPVVLSELGWMSMSIVDTIMVGR
                     LSAEAIGAVGMGNAVYYAPALFGIGILLGLDTLVSQAYGRGDFDECHRWLSQGVYLAI
                     GFTPILMLLVWAAPLAFPYLGVNLVVSQQSTDYLRILNWGTLPLLLYAAFRRYLQGVK
                     RVKPVTFALISANLINWAGNWALIYGHLGCPAMGVRGSALSTCLARVYMAAVLIFASW
                     KNEAGRGHPLFAHWPGLALSRIRALMKLGIPAAGQIVMEVGAFGAATVMAGKLTPVAL
                     AAHQIALNCASFTYMVPLGTSAAAAVAVGHAVGAGHGSQARRAGWLALAVGAGFMACA
                     AIVFLLAPRLILDIYTNQEVVVGTGVRLLALAAAFQIFDGIQTIATGALRGLGETRAP
                     MLANFAGYWIFGLPLGYILCFVLGRGILGLWTGLTLALVLIAMLLLWKWSRHSSNLRY
                     SPACP"
BASE COUNT          719 a          969 c          989 g          804 t
ORIGIN      
        1 tcggcatgaa cctcggcgga acatggcgcc atgaagacgt ggccgtcagc atggtagagg
       61 caaagcacag ctctggcatt gtggacggcg acttgaaaat ctatggcgga gaaccttgcg
      121 gctttgtact gaccattgaa aacggcccgg tgctctacca tgcaggcgat acggccgttt
      181 tctctgacat gcagcttgtc cgcgatctgt acaaaccgca attggcaatg cttccgatcg
      241 gcgaccacta taccatgggc ccgaaggccg ccgcgcttgc cgcaaaattt cttggcgtga
      301 agaccgtatt accgattcac tacggaacat ttccggtact tacagggact ccagaagaat
      361 taagaaaaca tcttgaagga agcggaattg aagtgaaggc gatttcgccc ggagaaagca
      421 tccggtaaca gcaaaaatct gccggcatac cagccgtact ggatgtggaa atacggctgg
      481 tatggcaatt ccggagcgcg gcccttcagc attcgattct gtgtcccctg cggaagctgt
      541 gcgtctgctc atacacattt tctgctctgg attgccggac atccgaagac ggtattcttt
      601 tgaaagagga aaaatgaaag ctctggtaaa aagcaggccc gaacgcggcc tttggattga
      661 ggatgttccc gagccgcaga ccggaatcaa tgatgttaag attcgcgtgc tgatgacagg
      721 catctgcggc acagacctcc acatttatga gtgggatgcc tgggcgcaga aaactatccc
      781 ggtacctctg gtcataggac atgaatttgt cgggcaaatc gttgaggtcg gctccaatgt
      841 cgccgatttc caaccgggag atctggtcag cggtgaaggt cacgtcgtct gcggccgttg
      901 ccgcaattgt cttggcggac gccgccatct gtgcgcgcat acgcagggcg tgggcgtcaa
      961 ccgcgccggg gcctttgcag aatatgtcgt tctgccgatg accaatatct ggcgccacgc
     1021 gcctggtatc ccgctcgatg ttgccgctat ttttgacccc cttgggaatg cggtacacac
     1081 tgccgcggct ttcccggtgc tgggcgaaga cgtgcttgtc acaggcgccg gccccatcgg
     1141 cattatggcg gcagcagtcg caaaacatgc ggcagcgcgt cacgtcgtta tcacggatct
     1201 gaacccatac cggctggaac ttgcgcgtat ggcggcaggc gtaaccgagg caatcaatcc
     1261 gcgagaaaca acattggagc aggtgcagaa gcagctaggc atgcaggaag gcttcgatgt
     1321 gggccttgaa atgtcgggca atcctcacgc ctttcgcgac atgctggcca atctgagtca
     1381 cggtgcaaaa attgcgatgc tcggcattcc ttctccatcc gaaatggcaa ttgactgggc
     1441 cagggtcatc ttcaatcagt acacaattcg cggcatttat ggcagggaaa tgtatgaaac
     1501 ctggtacaaa atgacagtca tgctacagtc cggacttgat atttcgaaag ttatcacgca
     1561 caggttcgca tggaacgagt tcgagcaggg ctttgcagcc atgcgctccg gaaactccgg
     1621 caaggtcctt ctggactggc agcatctttc ctgaagctga tgaaacttgg gcctgtgagt
     1681 ggcggagaga gtgggattcg aacccacgat acgctttcac gtatacacgc tttccaagcg
     1741 tgcgccttca gccactcggc catctctccc ggggggaagc gttcttactt aacgttaaca
     1801 tatttttgtt gtgcgcctgg gcgcggaaaa cctctcggaa caatcgcatc cagacgcagt
     1861 gagcaatcca cacgcgcatt cgttacattg gaaggatgcg tgtccagcca caggataaaa
     1921 acattacgag ggaactgcgc gatctgctcg ctctggccat tcctgttgtg ctttcagagc
     1981 tcggctggat gtccatgtct attgtggaca ccatcatggt tggccggctc agcgccgagg
     2041 ctattggtgc tgtcggcatg ggcaacgccg tctactatgc gcccgcgctc tttggcattg
     2101 gcattttgct tggactcgat acgctggtct cccaggcata cggacgcggc gatttcgacg
     2161 agtgccaccg ttggctttcg caaggcgtct atcttgccat tggattcacc cccattctga
     2221 tgctgctcgt atgggctgcg ccattggcgt ttccatacct gggcgtaaac ctggtagtca
     2281 gccagcaaag cacagactat ctgcgcatcc tgaactgggg aacattgcct ctgcttcttt
     2341 atgccgcctt ccggcgctat ctgcagggtg taaagcgggt caaaccagtc actttcgcgc
     2401 tcatttccgc gaacctcatc aattgggcag ggaactgggc gctgatttac gggcacctcg
     2461 gttgtccggc catgggagta cgcggctcag cgctttctac ctgcctggcg cgcgtgtata
     2521 tggctgctgt gctcatcttt gcctcctgga agaatgaagc cggacgtgga catccactct
     2581 tcgcgcactg gcctggattg gccctcagcc gcatccgcgc actgatgaag ctcggcattc
     2641 cagccgcggg ccagatcgtc atggaagtcg gagcattcgg agcagctacc gtaatggcgg
     2701 gcaaactgac gcccgtggcc ctcgctgcgc atcagattgc actcaattgc gcttccttca
     2761 cctacatggt cccgctaggg acgtcagccg ctgctgccgt tgccgtgggg catgcagtcg
     2821 gcgctggtca tggctcgcag gcgcgccgcg ccggatggct cgcgctggcc gtgggcgcgg
     2881 gttttatggc ctgtgccgca attgtcttcc tgcttgcgcc gcggctgatt ctggacatct
     2941 acaccaacca ggaagttgtc gttggaacag gcgtgcgcct gctggcgctg gccgctgcct
     3001 ttcagatctt tgacggaatc cagaccatcg ccaccggtgc tctgcgcggc ctgggagaga
     3061 cgcgcgctcc catgttggcg aactttgcag gatactggat cttcggcctg ccgctcggct
     3121 atatcctgtg ttttgtgctg ggccgcggga ttctgggcct ctggaccggg cttacccttg
     3181 ctcttgtcct cattgccatg ctcctgttat ggaaatggtc acggcactct tccaatttga
     3241 gatacagtcc agcttgtccc tgagataata aactacttcc taactggatt tttgtccgca
     3301 ttacttcaaa acaggattac ttgagcagag aacaacaaaa actccatggc tgatacatat
     3361 aagatcgcaa tcgtaggcgc agcctcactg cgcggcaaag aactgaatga agcgctgggc
     3421 gattccgcgt tcggcgcatc cgattttgtg ctgatggacg acgagtcatc cctggggcag
     3481 c
//
LOCUS       DASRBV010000052        23553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_407662, whole genome shotgun sequence.
ACCESSION   DASRBV010000052 DASRBV010000000
VERSION     DASRBV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 23553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23553
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407662"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VFA02_02210"
     CDS             complement(<1..1508)
                     /locus_tag="VFA02_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU08689.1"
                     /translation="MPEKEVNPWLIAVSVMLATFMEVLDTAIASVALPYIAGSLSAST
                     DEATWVLTSYLVANAVILPASHWFSLRFGRKRFLLSCVVIFTIASFFCGAAPSLAFIL
                     IARVIQGAGGGALQPLSQAILLESFPPKKRAAAMAVFAFGVVVAPILGPTLGGWLTDT
                     YSWRYAFYINIPVGALAIFMINRFVHDPPYIKNAKASPFDNLGFGLLAVWTGCLQVIL
                     DKGQEDDWFGAVWIRWATLVAISTFLWFIVHSWRKKEPLVNLRTLKDWNFAIGCALML
                     LLGLSVYSTITVLPLFYQEVLGYTAFTAGLVVGPRGFGAIAAMPIVGTLGSRIDARFL
                     LTFGFGVFGVTSLIFGRVTPDTSPTTLLWPIIITGFAMSFVFVPITTQAYATLKNEQI
                     GNASGIFNLVRNIGGSVGISVAQTLLTRRADLHQHDLAAAISRSNYWLQQRMAGLTSY
                     LSHQTNSANAAAGSQAQIYQLLGHQSTLWAFVDVFRYIALTCFGCTLLVWMF"
     gene            1709..3649
                     /gene="dnaK"
                     /locus_tag="VFA02_02215"
     CDS             1709..3649
                     /gene="dnaK"
                     /locus_tag="VFA02_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZU08690.1"
                     /translation="MGHKFMAKIIGIDLGTTNSVVAVMEGGEPKVIANEEGGRTTPSV
                     VAFTKSGERLVGQVAKRQAITNPHNTIYSIKRFMGRRYNEVNDEMKMVPYKVVQQGDH
                     VAVDVEGKLYTPPEISAMILQKLKKAAEDYLGTSVTEAVITVPAYFNDAQRQATKDAG
                     KIAGLDVKRIVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVIEVKST
                     NGDTHLGGDNIDQRIVDWLIDEFKKDEGLDLRSKGNEMALQRLKDAAERAKIELSTAL
                     ETEINLPFITADASGPKHLVKKLTRAKLEQLVEDIIQRSVEPCKKAMADAGVDASKID
                     EVVLVGGQTRMPRIQQLVKELFGKEPHKGVNPDEVVAVGAAVQAGVLSGEVKDLLLLD
                     VTPLTLSIETLGGVATPMIPRNTTIPTKKTEVFSTAADNQTEVEVHVLQGERPMAAQN
                     RTLGKFKLGGIPPAPRGVPQIEVTFDIDANGILNVTAKDRATGKDQKITITSSSGLSK
                     EEVERMAKEAEAHSAEDKAKREEIEARNQLDNLVYNIEKMLKESGDKVSGSDKTDVES
                     ALAEAKKTLEGSPSVSELNAAREKLTAASHKLAEAMYKASAAGATAGAPTDGAQAAAG
                     TTDEKKDEGVIDAEYVDVDEKK"
     gene            3768..3938
                     /locus_tag="VFA02_02220"
     CDS             3768..3938
                     /locus_tag="VFA02_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08691.1"
                     /translation="MNETLWKCEQLRAGQVYTTLMFNTREEAEKFKAQMLRMAPDMFC
                     RIEPIPVKMVWN"
     gene            3991..4788
                     /locus_tag="VFA02_02225"
     CDS             3991..4788
                     /locus_tag="VFA02_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZU08692.1"
                     /translation="MPEGNEIHAFAQRHAAMFAGKKVRVDSPNGRVSDAEILDGRRLL
                     GVDAVGKHLGYNFGRDLVLHVHLGMYGDFREGKMPLPEVKGAVRLRIYTKTDWLELRG
                     GTDTSIFSHTKWESLLKRLGPDPLRPGAVPSPGFEKILKRNTPVGALLMDQSVFAGIG
                     NIYRAELLFRARMNPWRPGKNTTLVELKAIWKDAKKLLSDGVKNTRYVTTLPKDRPHK
                     NHKPVLEEELHYVYRRHGKPCFLCGTKIGRAEMAGRTVYWCPNCQKT"
     gene            complement(4876..5343)
                     /locus_tag="VFA02_02230"
     CDS             complement(4876..5343)
                     /locus_tag="VFA02_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZU08693.1"
                     /translation="MQLKKLIFAGGILLASAMGACAQIVRIPLPNSDFPISQGVWVGD
                     TLYLSGMIDSSIMHGTAGDTKTQTINVMKQIQKALEDQKLTLGDVVMMHVYLAGDAAK
                     GGKMDFAGFMQGYTQFFGTKDQPNKPARSAMQVAALAAPAALVEIEVIAVRPK"
     gene            complement(5333..6904)
                     /locus_tag="VFA02_02235"
     CDS             complement(5333..6904)
                     /locus_tag="VFA02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HZU08694.1"
                     /translation="MGLTRRDFLTKVGKAGGYSATFLVMQQLGLLPVANAEAQELKPV
                     AGNGTQIVILGGGIAGLVSAYELGKAGYRCTVLEARQRVGGRNWSIRNGTKVNFSDGT
                     EQTCAFSRDHYFNAGPARLPSIHTTMLGYCRELGVPLEVEVNTARGTLLQSDRMNGGT
                     PVEQRQMVNDTRGHVSELLAKSIRKGALDEDMTAEDKERMLAFLREYGDLDPDLIYKG
                     SQRSGFKVCPAAGPEKPEPKDPLPMHALLDADLWRGLLVEDMIDWQATMFQPIGGMDR
                     IPAAFEKRLGGVIRYGAEVKQIRQSSAGVTVTYINTKTGKPETVTADYCICAMPLSVV
                     KHLDADFSPDVKALLEGVSYDSGYKIAWESPRFWEKKYKIYGGLSYLQQTVDVVWYPS
                     AAFFSETGIIIGGYSIEDGTPFGKLQNMQAKLDASRAAIEKLHPGHSQYLKNPVYVNW
                     GKIPFNYGSWIHGRDEDYETIYQRVIRPDRRVYFAGDHTSRLIGWQEGAALSAYRTIN
                     QIGAAMQNGGQTSAA"
     gene            complement(6975..10283)
                     /locus_tag="VFA02_02240"
     CDS             complement(6975..10283)
                     /locus_tag="VFA02_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08695.1"
                     /translation="MMLLPSRRISFQTIAVAFLFVVFLWLGRAIDAQTFRGGINGVVT
                     DQAGAVISGAQVVATADDTQVAHTTITSSAGEFSFQDLPLGSYTVTASASGFQKLKVG
                     KVTVTAGTIYTLPMKLPVASEATTVEVSAAGLALDTTTTTQTTVIPDKTVQDIPLNGR
                     DFTQMIGLAPGFAGYSLGGYGSVNGTRANQVNWQIDGADNNDWWHNIPAVNQGGVENI
                     AGVTLPIDSIAEFSLQTQSAPETGRNPGGTVNLVTKSGTNQLHGTAYYYERNQALAVV
                     SPFVQGNPPLENVQWGASLGGPFWKDHTFWFANFEKQKFNIATGNAGTEPGVLYQQKA
                     QALLQQYNVPVNSATNALLSYLWPADLVNSSVPASPNNFSSAAPEFGYSYNGVMKLDH
                     TFNDRHSLSARAFLGQGNQTAPVGSTDVNPWYFEVGPIHVYNYSVAHNWVISPRMTNQ
                     VTIGVNYFNQTFSDAKTGFDVGSVGFVTNSPYSQAPNIQINGFESTGNTPPEGRNDIT
                     GHLDEAFSWTFGKHQLRFGGEYRQAQINEFYKRHSTGSFKFTGTAGPWNTSDGSCVVS
                     DSNVCALADFMAGYLSLQSIVVGNPERFVYSHAFNLFAQDSWQLTSQLNINYGVRYDF
                     MQPMYNHDKNLSVFRPGLTPDGLAFQGADISQVYPSDWLSISPRVGFSYQLSGNAGIV
                     VRGGFGMFFDTPNANPFLDNRPGNSAPNGLEGNPGGSDPVYTLTTAQYSTTINGNTVG
                     LVVPGQPIIQTTSLVCNASSQCGVFSVNKNFRSPYNFNFNLQIEKSLGSKATVQLGYV
                     GSEGRRLLSLLNINQPYLGAFNSGYTGQYALPYQAQYPGYSDINQIESIGTSNYNSLQ
                     AVLRSNNWHGLTAQAAYTWGHNLDEVTAYRGALPQDSTNFKGDYGNSDFDTRNTFVGY
                     ANYDIPVFKGPSRLTHGWQVNTAFTFKGGQPITIYNANDTSGTNEYTQRVNQVANPFA
                     GVSHKIITPVGGGTPYVQWFNPNAFVQPAANTWGTIARNSLYGPGFSDVDLSLFKNTS
                     LTEKINTQLRAEIYNLFNRLNLANPGCSQLCNAYLGNGGFGTIGSTIGSGNYSPGIGP
                     GEPFNVQLALKIIF"
     gene            10948..11691
                     /locus_tag="VFA02_02245"
     CDS             10948..11691
                     /locus_tag="VFA02_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08696.1"
                     /translation="MKAGFAVCALWISGGLLFAQDATSRQKLDLPKAPTLVMVKTLPD
                     KGTLVMQVNVGGVNIVRSDMEKTIRLTIDPKAFYDDATQITQTRFIHRVPTIDPKAFY
                     DDATVKSWVRRFDVAGDRAWIDLKLPDHGNNGNHHHSSPEITIAVPAQTDLKLELGVG
                     QLTVKGVEGNKELHVGIGELDVGLSDASQYHEIQTSSKLGEADDDTSNQHSGGFFPKT
                     HHTSVQGPYKLHATVGIGQVNVARNANER"
     gene            complement(11786..12406)
                     /gene="purN"
                     /locus_tag="VFA02_02250"
     CDS             complement(11786..12406)
                     /gene="purN"
                     /locus_tag="VFA02_02250"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZU08697.1"
                     /translation="MSAPKRLGILLSGRGSNFIAIADAITSGKLQGAEIAVVISNKAD
                     APGIAAARERGLSTHVIEARGRRRSEHDAEIIACLREHHVDLVCLAGYMRLLSPEFVR
                     AFPQRILNIHPSLLPAFPGLDAQQQALEYGVKVSGCTVHFVDEQLDHGVIVLQKTVSV
                     LDEDTAETLSARILEQEHIAYTEAIARVLSGKYEITGRRYLPVEPV"
     gene            complement(12403..13446)
                     /gene="purM"
                     /locus_tag="VFA02_02255"
     CDS             complement(12403..13446)
                     /gene="purM"
                     /locus_tag="VFA02_02255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZU08698.1"
                     /translation="MPEQISSVTYADAGVDITSGDRAKQRIKYLAQKTFTRNVLGEIG
                     GFGGLFKLDTAKYQQPVLVSSADGVGTKLKVAFELGIHHTVGADLVNHCVNDIAVQGA
                     TPLFFLDYLATGKLDGAVTEKIVSGLADACRANGCALIGGETAQMPGFYADGEYDLAG
                     FIVGIVEKDRIITGAHIQPGDVLIGLPSTGLHTNGYSLARKLFFEVAGYKAEQYVNAL
                     KEKAGAALMKIHRSYLNVIKKLTASEYVRGMAHITGGGITENLPRILPKNISAVVELG
                     SWPVLPIFEHLRDLGNVPQDEMLRTFNMGIGLICAVPADKFKRVKSTLERANEKFYVI
                     GRTVKGDRKVVYQ"
     gene            13950..14732
                     /locus_tag="VFA02_02260"
     CDS             13950..14732
                     /locus_tag="VFA02_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08699.1"
                     /translation="MHLNLLDDILWAASFFGNVVLLAVLLIKRRWKEFPVFTILIADF
                     AIETPALFLIYRYSSLHWYAIIYWTTYTLDFVLQVALVFEMARIVLRPTGTWVRDARS
                     TFLLCGVAGAAIALVLTYIVHPVAPSSLVAWGVRAYLFSSLLFCELFFAMMLAAQRLG
                     LVWRNHVMGLGQGLTAWSLATMLVHAAHTYFGAVNKYVFNTLEHVRIIVYISATIYWV
                     ITFWLPEPERRPLSPEMQKYLVALHDKVQYDASQVSGAQNPR"
     gene            14742..15272
                     /locus_tag="VFA02_02265"
     CDS             14742..15272
                     /locus_tag="VFA02_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08700.1"
                     /translation="MIPILLLLTFVVLLAAGLVWMQISNHRLHSLSWEDLVSQLQPVP
                     ARGLEIVALDYMEPRPNQLKLEPDHLWAFIGGMEGLHGMRRNANLLIALAVHVQRWNF
                     EEGVIVTERMRQDAVQLKRALFRIRIEMLLRRTQLRTPFYIQQAASSYYLMTKRLLAL
                     YETSHAGLLPVLKAAI"
     gene            15316..16614
                     /gene="hemL"
                     /locus_tag="VFA02_02270"
     CDS             15316..16614
                     /gene="hemL"
                     /locus_tag="VFA02_02270"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZU08701.1"
                     /translation="MRTLDRSLALQARAEKLFPGGVDSPVRAFRAVGGNPPFLVKGEG
                     AHLWDADGNRYLDYFGSWGPMILGHAFPPAVEAIRKAAAESASFGASTPAEADLAEAV
                     AQAFPSMEKMRFVSSGTEATMTAIRLARAFTGRKYVVKFEGCYHGHSDGLLVKAGSGV
                     ATFGIPGSAGVPEEIAHFTLALPFNDVPAVEAAFAKHRAEIACIIVEPVVGNMGCVPP
                     AEGFLQSLHEIKQREGALLIFDEVITGFRLAFGGAQEVYGIRPDLTTLGKILGGGLPC
                     GAFGGRTEIMDMLAPLGPVYQAGTLSGNPLAMAAGIATLSYLKEHRGEVYPQLEKLAA
                     AVANGVAAVAAKAGVPVAVNRVGSMFTWFFTPERVTDFASASSSDTAAYARFHRAMLE
                     GGVWLPPSQFEAAFVSTAHTMDDVDTTIAAAREALAVWTR"
     gene            complement(16651..17451)
                     /gene="mutM"
                     /locus_tag="VFA02_02275"
     CDS             complement(16651..17451)
                     /gene="mutM"
                     /locus_tag="VFA02_02275"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZU08702.1"
                     /translation="MPELPEVETVARGVHERVRGETVESVWFSSKPEPFKTSPRKMAR
                     ALTGKKIERVYRVGKHIVFDLSQDTQWIVHLGMTGRLLACNSADSVANHTHAVLHFSS
                     GRELRFIDPRRFGRLDIRERTAFTGPGSEPLTISFEEFAELFRGRKTSIKAALLNQKL
                     LHGVGNIYADEGLFRAGIRPTRMAKSLKKKELNLLHSALQTVLKDAIRLGGSSVSDYV
                     DANGARGFFQLEHKVYLRGGRPCLVCGTAIRRLVLAGRGTHYCPRCQK"
     gene            17520..18680
                     /locus_tag="VFA02_02280"
     CDS             17520..18680
                     /locus_tag="VFA02_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU08703.1"
                     /translation="MSDATPLTQLNEDEQLFRSTVREFAQQTIGPLVRQMDEEQHFAA
                     ELLPQLFSLGLMGIEVPVEYGGSGGTFFEAVLAVEEISAVDPAVGVLVDVQNTLCINA
                     LLRWGTEAQKKTYLPKMATDTVGAYALSEAGSGSDAFALQTRAELRDGHYVLNGQKLW
                     ITNAKEAGLFIVFATLDASAGYKGITAFLVEKDTPGFSVGKKEDKLGIRASSTCELIL
                     DDCRVPQENVLGEPGKGYKIAIETLNEGRIGIGAQMLGLAQGAWNCAAKYAQERRQFG
                     KPIAEFQAVQFALAEMATKIEATRLLVYNAARLKDAKQSYIREAAMAKYFSSQVAERV
                     ASQAVEIYGGYGFVKDYPVEKFYRDAKIGKIYEGTSNMQLATIGKLVLGGRG"
     gene            18836..20650
                     /locus_tag="VFA02_02285"
     CDS             18836..20650
                     /locus_tag="VFA02_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU08704.1"
                     /translation="MLGLYQRLILGFLLLIALVGGLGMLVRHSFVHLSALDEQQQTED
                     SAVSALAQIQASLAREQVLTGRLAVNPARPVYEQLMAQAEVTEQRFVAAVRTQAPALG
                     QLEQKHRQVVERLRGNLNNPNAWRDLTANVFRPQLDTIDEGLSDNLRDLFVRRASLMA
                     DLDHERSMLRARLIAISGFSLFVALAISAITTFLVIRPIRATTRTAQQIGQGDLHRRI
                     EWTMNDDLGALATEINRMAIRLRDLRETESGRRQMEQQLSDAVINSIFEPVIVTDAKG
                     HVLKLNQAATELLGKSDRMALANTPGGEKILNAVRNAVSMQRATTGEGEAALLPMRIG
                     SAERSYRLRTAPMRDADGKLLGAVTVLEDVTEMRDIDRFKTRFLSVASQKLRDPLQRL
                     RLALYALTRGHAGELRPLQFEILEDATQETERLDDLMADLIEVAELDTGRRQMKIERL
                     RPIDILEDACHRFREQAREKKIEIEVEAFADLSYVNADRRALRSILDNLLSNALRYTP
                     EGGQVRLKASELKDRIQFVVQDSGRGIEAERLPTIFGRFAGTSSDGTGLGLALVRRLV
                     ETLGGQVSVESRLGQGTTFSFTLPIATPAPARHEVEVG"
     gene            20653..21738
                     /locus_tag="VFA02_02290"
     CDS             20653..21738
                     /locus_tag="VFA02_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZU08705.1"
                     /translation="MDKGKLRVYLGAAPGVGKTYRMLEDAHLLRQQGVDVVIGLIETH
                     GRKDTAALIGDLEVIPLKEVPYRTVTLREMDIEAILARHPHTCIVDELAHSNVPGSRN
                     RKRYEDVLELMDAGINVMTAVNIQHLETLNDAVSRSASTQIRETVPDSFLKRADEVVN
                     VDVTIEELRGRLREGKIYAPEKIEQALANFFRKGNLNMLRELALRTTAEQVSTAASEY
                     RRIQGLEQAPIPEKVMVCISTRPGTERLIRLGARIAGRLATNWYVVYVDTEKDHHEDP
                     EARHRLEEYQRMSRDLGAKVIVLKDKNVADALINFTKKENISHVIFGQSARSRFEILL
                     HGSVINRFLAEVRDTTVQVVPMSREKK"
     gene            complement(21783..>23553)
                     /locus_tag="VFA02_02295"
     CDS             complement(21783..>23553)
                     /locus_tag="VFA02_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZU08706.1"
                     /translation="ARIRRREVIKLLRPQGREYGELFAGNGMGKKLLSFHAWSIAPDG
                     HPYTVRDNEIREVGADGGGMLYVDYRAKVATPPGADPGGVVASESENLLPQYMKESSW
                     DFQTDIPVARAVFEVDLPTGWKQRAVWHHYAAVQPQEIAPNHWRWELAHVPAVDLEDV
                     PLPPPEGALEGRMVVHYAAGDIPQGDQLWTSIGQWYDDLATSRSETTSEIAAKAQSLV
                     GGEGDFTARVEKIAEFLQRDIRYVGIEIGIGGYQPHPAADIYRNRYGDCKDKATLLIA
                     MLRAIGVHATYVLVDTRRGFVDADVPSIDGNHAIAAIELPQGYNDARLKTVVKTRAGE
                     HFLIFDPTNEYVPLGLLPTYLQGSYGTLVNGAHSQVVQLPVVPPDADVTEHTGKFTLK
                     EDGTLEGSVLETRTGASAAAERHYFALNAGKERQQYLERRLQQDFSDFALASENAENA
                     QNLEKDFVLHYQFTAQGYAKHAGDLLLVRPRVLGSVAEALDDKERKYPVNLEREGTWR
                     DSFTITLPSGYTVDETPEPVKIDAGFASYQSEVKVSKNELRYSRELVVRQLELPASEY
                     DALKKFEGAITADEHRSAVLRKE"
BASE COUNT         5312 a         6297 c         6813 g         5131 t
ORIGIN      
        1 ctgaacatcc agaccagcaa agtgcagccg aagcaggtga gggcgatgta gcggaagacg
       61 tccacaaagg cccagagggt ggactggtgg ccgagcaact gatagatctg cgcctggctt
      121 ccggcggcag cattggccga attagtctga tggctgagat agctggtcaa cccagccatg
      181 cgctgctgga gccaatagtt ggaacgcgag atggccgcag ccagatcatg ctggtggagg
      241 tcggcgcgcc gcgtcagtag cgtctgggcc acggaaatac cgacagagcc gccgatattg
      301 cgcaccagat tgaagatgcc gctggcattc ccgatctgct catttttcag cgtggcgtag
      361 gcctgcgtgg taatcggcac aaagacaaag ctcatggcaa agcccgtaat gataatgggc
      421 cagaggagcg tcgtcgggct tgtatcagga gtgaccctgc cgaaaatcag cgacgtaact
      481 ccaaaaacgc caaagccaaa cgtgagcaga aaacgggcgt ctatcctgct ccccaaagtg
      541 ccgacaatgg gcatagcggc aatggcgcca aatccgcgcg gccctaccac cagaccagcc
      601 gtgaaggcgg tataacccag cacctcctga tagaagagtg gtaggactgt aatcgtggaa
      661 taaacgctca gcccaagcag cagcatcagg gcacagccga tggcaaagtt ccagtccttt
      721 aacgtgcgca ggtttaccag aggttctttt ttgcgccacg aatggacaat gaaccacaga
      781 aaggtgctta tggccacaag agtggcccag cggatccaga ctgcgccaaa ccagtcgtcc
      841 tcctgccctt tgtccagaat cacctgaagg cagccggtcc acacagccag cagaccgaag
      901 ccgaggttat caaacgggct ggccttggca tttttgatat atggcgggtc gtggacgaag
      961 cggttgatca taaagatggc cagcgcgccc acggggatat tgatgtagaa ggcatagcgc
     1021 cagctgtagg tatccgtgag ccagccacca agcgtgggac cgaggatggg ggccacgacg
     1081 actccgaagg cgaagacggc catggcggca gcacgctttt tcggaggaaa actttccaga
     1141 agaatggcct gggagagcgg ctgcaaagcg cctcctccgg ccccctgaat cacacgcgca
     1201 atcaaaataa acgcgagaga tggtgcagcc ccgcagaaaa aagaggcaat cgtaaaaatg
     1261 acgacacaac tcagcaagaa gcgcttccgg ccaaatcgta aagaaaacca atgacttgca
     1321 gggagaatga cggcattggc gaccagatag ctggtcagga cccaggtggc ttcatccgta
     1381 gaagctgaaa gcgagccggc aatatatggc agggccaccg acgcgatggc cgtgtccagc
     1441 acctccataa aggtggccag catgacggaa accgcaatca gccagggatt tacctctttt
     1501 tcaggcataa gtacggcaaa cgagcggaat attccttaga tacaaacgcg cactgattgg
     1561 attcatattt cgtggaatca acgacttaca ttttctaaaa aatccagcat ccagcctgtg
     1621 aaattcgaca tcagataaat tgacaacaaa agactcaagt gcgaaactgg ctagagcaag
     1681 tcaactcaaa gccgcagcac agaaggcagt ggggcataag tttatggcaa aaattatcgg
     1741 aattgacctg ggcacaacga actccgttgt tgccgtcatg gaaggcggcg agccgaaggt
     1801 aatcgccaac gaggagggcg gccggaccac gccttccgtc gtggccttta ctaagagcgg
     1861 cgagcgcctg gtgggacagg tggccaaacg gcaggccatc accaacccgc acaacaccat
     1921 ttactccatc aagcggttca tgggacgccg ctataacgaa gtgaacgacg agatgaagat
     1981 ggtcccctac aaggtggtcc agcagggcga ccacgtagcg gtggatgtgg agggtaagct
     2041 gtacactccg cctgagatct cggcgatgat cctgcagaag ctcaagaagg cggcggagga
     2101 ctatctcggc acatcagtga ccgaagccgt catcaccgtt cctgcctatt tcaacgacgc
     2161 gcagcgccag gccacgaaag atgcaggcaa gattgcaggt ctggacgtaa aacgcattgt
     2221 gaacgagccg acggcagctg cgctggccta tggcctcgac aagaagaagg acgagacgat
     2281 tgccgtatac gacttcggcg gcggcacgtt cgatatttcc attctcgaag tcggcgaggg
     2341 cgtcattgaa gtcaagtcca cgaacggtga cacccacctc ggcggtgaca acattgacca
     2401 gcgcatcgtg gactggctga ttgacgaatt caagaaagat gaaggccttg acctacgctc
     2461 gaagggcaat gaaatggcgc tacagcgttt gaaagatgcc gcggagcgcg ccaagatcga
     2521 gctttcgacc gcgcttgaga ccgaaatcaa tctgccgttc attacggcag acgcgagcgg
     2581 tccaaagcac ctagtcaaga agctgacgcg cgccaaactg gagcaactgg tcgaggacat
     2641 cattcagcgc tcggttgagc cttgcaaaaa ggcgatggcc gatgcgggtg tagacgcgag
     2701 caagattgac gaagtcgttc ttgttggcgg acagacgcgc atgccgcgca tccagcaact
     2761 ggtgaaggag cttttcggca aggaaccgca caaaggtgtg aacccggacg aagtagtagc
     2821 cgtgggcgct gcggtacagg ctggcgtgct gtcgggcgaa gtgaaagacc tgctgttgct
     2881 cgatgtgact ccgctgacgc tctcgattga gacgctgggc ggtgtggcga cgccgatgat
     2941 tccgcgcaac accaccattc ctacaaaaaa gacggaggtc ttctcgaccg cggccgacaa
     3001 tcagacggaa gtagaagtgc atgtgctgca gggcgagcgt cccatggcag cgcagaaccg
     3061 cacgttgggc aagttcaagc tcggtggaat tccgcccgcc ccgcgcggtg tgccgcagat
     3121 tgaggtgaca ttcgatattg acgcgaacgg catcctcaac gtgacggcaa aggaccgcgc
     3181 tacaggcaaa gaccagaaga ttaccatcac gtcgtcttcg ggtctcagca aggaagaggt
     3241 cgagcgcatg gcgaaggaag ctgaagcgca ttcggccgag gacaaggcca agcgtgaaga
     3301 aatcgaagca cggaaccagc tcgacaacct cgtctacaac atcgaaaaga tgttgaagga
     3361 gagcggcgac aaggtttctg gcagcgacaa gacagatgtt gagtctgcgc tggccgaggc
     3421 caaaaagacg ctcgaaggct cgccttccgt aagcgagctg aatgcggcac gggagaaact
     3481 gaccgctgcc tcgcacaagc tggctgaggc catgtacaag gccagtgctg ccggcgcaac
     3541 cgcaggcgct cccactgacg gagcacaggc ggccgctggt actacggatg aaaagaagga
     3601 cgaaggcgta attgatgccg agtatgtaga cgttgacgag aagaaataac accgttcaag
     3661 gtgtcatccc tgagcgcagg cgaagggacc ttcctgaggg cgcgctcgac cgaaacgttg
     3721 acgcgccctg cgtgtttata agagtgaaga ctggagtagt tgcagttatg aacgagactc
     3781 tttggaaatg cgaacagttg cgcgccgggc aggtgtacac cacccttatg ttcaatacca
     3841 gggaagaagc tgaaaaattc aaggcgcaaa tgttgagaat ggccccggac atgttttgcc
     3901 gtattgaacc catcccggtg aagatggtct ggaattaaaa ccagcttctt ccctttctcc
     3961 ataacggcac tctgttataa atcgattgcg atgccggaag gaaatgaaat ccatgctttt
     4021 gcgcaacgcc atgcggccat gttcgcaggc aaaaaggtgc gtgtggattc gcccaatggc
     4081 agggtttcgg atgctgaaat cctcgatggc cgcaggctgc ttggcgtgga cgcagtcggt
     4141 aagcacctcg gctacaactt tggtcgcgac ctggttctcc acgtgcatct gggcatgtat
     4201 ggagacttcc gcgaaggcaa aatgcctttg ccggaagtga agggtgcggt acggctgcgc
     4261 atctatacca aaacagactg gctggaactt cgcggcggaa cggacacttc catcttttcg
     4321 cacaccaaat gggagtcgct gctgaaacgg ctcgggccag atccgctgcg cccgggtgca
     4381 gttccttcgc ccggttttga aaaaatcctg aagcgcaaca cgccggttgg cgcactcctg
     4441 atggaccagt ctgtctttgc cggaattggc aacatctatc gcgcggagct gctgtttcgc
     4501 gctcgtatga atccatggcg tcctggaaag aacaccactc tcgtggaact gaaggccatc
     4561 tggaaagacg cgaagaagct gctgagtgat ggcgtgaaga acacgcgcta tgtcacgacc
     4621 cttcccaaag accgtccaca caaaaaccac aagcccgtgc tcgaagaaga actgcactac
     4681 gtttatcgtc ggcatgggaa gccttgcttt ctgtgtggca caaaaattgg gcgcgcagaa
     4741 atggctggac gcacagtgta ctggtgcccg aactgccaaa agacctgaaa atatcactgt
     4801 cagagaggta cgaagtaaac gcaggtccag cagacagggt atgctgacta ctctcacacg
     4861 aaaaccgctc ggagatcatt tcggccggac ggcgatcacc tcaatctcta cgagtgcggc
     4921 aggagcagcc aatgctgcta cctgcatggc gctgcgtgct ggtttgttgg gctggtcttt
     4981 tgtcccgaag aattgcgtgt agccttgcat gaatccggca aagtccatct ttccgccttt
     5041 ggcagcatcg cctgcaagat acacatgcat catgactaca tcgccaagcg taagtttctg
     5101 gtcttcaagc gccttctgta tctgtttcat cacgttgatg gtttgtgttt tcgtatctcc
     5161 ggcggtgccg tgcatgatgg aagaatcaat catgccgctc agatagagcg tgtctccgac
     5221 ccagactccc tgggaaatgg gaaaatctga gttcgggaga ggaatgcgca cgatttgagc
     5281 gcaggcgccc atcgctgacg cgagcagaat ccctcccgca aagataagct tcttaagctg
     5341 cactggtttg tcctccattt tgcatggctg cgccaatctg gttgatggtt ctgtatgccg
     5401 aaagtgctgc accttcctgc cagccgatta agcggctggt atggtcaccg gcgaaataaa
     5461 cgcggcgatc aggacggatg acccgctggt agatggtttc gtaatcttca tcgcggccat
     5521 ggatccatga gccatagttg aagggaatct tcccccagtt cacataaaca gggtttttca
     5581 gatactggga atgcccggga tgcagttttt caatcgcggc gcgcgaggcg tcgagcttgg
     5641 cttgcatgtt ttgcagcttt ccgaaaggcg ttccgtcctc aatcgaatat ccgccaataa
     5701 tgatgcctgt ttctgaaaag aaggccgcgc tgggatacca gacaacatct acggtttgct
     5761 gcaggtagga aagaccacca tagattttgt attttttctc ccagaatctc ggcgattccc
     5821 atgcaatctt gtagcccgaa tcataactta ccccctcaag cagggccttt acgtcgggtg
     5881 aaaagtcggc atccagatgt ttgaccacgc tcagaggcat ggcgcagatg cagtaatctg
     5941 ctgtaactgt ttcaggtttg ccggtctttg tattgatata agtaaccgtg actccagcag
     6001 acgactgacg gatctgtttc acttctgcgc catagcgaat tacgccgccc agcctttttt
     6061 caaaggcggc aggaatgcgg tccattccac cgatgggctg gaacatggtc gcctgccagt
     6121 caatcatgtc ttcgaccagc aggccccgcc ataaatctgc atcgagcaaa gcgtgcatgg
     6181 gcagagggtc ttttggctct ggcttttccg ggcctgcggc gggacacacc ttgaacccgg
     6241 accgttgaga gcctttgtag atcaggtccg gatcaagatc accatattcg cgcaggaagg
     6301 ccagcatgcg ctctttgtct tcggcggtca tgtcttcatc gagcgcgccc ttcctgatac
     6361 ttttggcgag aagctcagag acgtgcccgc gcgtatcatt caccatctgg cgttgctcga
     6421 cgggagtgcc gccgttcatc cggtcacttt gcaacaaggt cccgcgtgcg gtgttcactt
     6481 ccacttccag tggaacacca agttcgcggc agtatccgag catcgtagtg tggatggaag
     6541 gcaggcgtgc cggacctgca ttgaagtaat ggtcccgcga gaaggcgcaa gtctgttcag
     6601 tgccatcgct aaagttgact ttcgtgccgt tacgaatgct ccagttgcgc ccgccaacac
     6661 gctggcgcgc ttcaagcaca gtacatcggt atcctgcttt gccaagctcg taggcagaga
     6721 cgagtccggc aattcctccc cccagaatga caatctgggt cccattgccg gcaactggtt
     6781 ttagctcctg tgcttcagca tttgcaacag gcagtagacc gagctgctgc atcaccagga
     6841 aagtggcgct gtatccacct gccttgccaa ccttcgtcag aaaatcacgt cgcgtcaatc
     6901 ccatttggtt gctctccgga gttgtttgtt cgcaacagaa ggacctttcc ggtcttccac
     6961 agaaaacgag caggttagaa gataatcttc agagcaagct gtacgttgaa gggttcacct
     7021 gggccaatac cgggtgaata gttgccggag ccaatggtgg agccaatcgt cccgaatccg
     7081 ccgttgccca ggtaagcgtt gcagagctgg ctgcatccag gattcgcaag attcaggcga
     7141 ttgaagaggt tgtagatctc tgcccttaac tgtgtgttga tcttttcagt cagagaagtg
     7201 ttcttgaaca aggaaaggtc tacgtcactg aatcctgggc cgtaaaggct gttgcgtgcg
     7261 atggtgcccc aggtgttcgc tgcgggttga acaaatgcat tggggttaaa ccactgcaca
     7321 tagggggtcc cgccgccgac aggagtgatg attttgtggc tgacgccagc aaatggattg
     7381 gcaacctgat tgacacgctg cgtatattcg ttcgtgccgc tggtgtcgtt cgcattgtaa
     7441 atggtgatgg gctggccgcc tttgaaagta aaggccgtgt ttacctgcca tccatgcgtt
     7501 aaacgggacg gccctttaaa gaccggaatg tcgtaattgg catagcctac gaacgtgttc
     7561 cgtgtatcga agtcgctgtt gccatagtcg cctttgaagt tggtgctgtc ctggggcaaa
     7621 gcaccgcggt atgcagtgac ttcatcgaga ttatggcccc aggtgtaagc cgcctgcgca
     7681 gttaaaccat gccagttgtt cgagcgcagg accgcctgca gagagttgta attggaagtg
     7741 ccaatactct cgatctggtt gatgtcactg tatccgggat actgcgcctg ataagggagc
     7801 gcatattggc ccgtatagcc ggagttgaag gcgccaagat atggctggtt gatattcagc
     7861 aggctcaata agcgccgacc ttcactgccg acatagccta gctgcaccgt ggctttgctg
     7921 cccagcgact tttcaatctg caggttgaaa ttgaagttgt agggagaacg gaaatttttg
     7981 ttgacgctga agacaccgca ctggctcgac gcattgcata cgagcgatgt ggtctggatg
     8041 atcggttgcc ccggcacgac gagtccgacc gtgtttccgt tgatggttgt gctgtattgc
     8101 gcagttgtga gcgtataaac cgggtccgat ccaccaggat tgccttccag tccgttggga
     8161 gcgctgttgc cgggacggtt atcgaggaag ggattggcat tcggagtatc gaagaacatg
     8221 ccaaagccac cgcgaaccac gatgccagca tttccggaga gctggtagct gaatccaaca
     8281 cgcgggctga tgctcagcca atcgctggga tatacctgac ttatatctgc cccctgaaat
     8341 gcaagcccgt caggtgtaag gcccggacga aagacggaaa ggttcttgtc gtggttgtac
     8401 atcggctgca taaagtcata gcggacgccg tagttgatat tgagttggga agtaagctgc
     8461 cagctgtcct gggcaaacaa gttaaatgcg tgcgaataga caaagcgctc gggatttcca
     8521 accacaatgg attggagcga cagatagccg gccatgaagt cggccaatgc gcacacattg
     8581 gaatcggaga ctacacatga gccgtcgctg gtgttccagg gacctgcggt tcccgtgaac
     8641 ttaaagctgc cggtcgagtg ccgcttatag aattcattga tctgggcttg gcggtattcg
     8701 ccgccaaacc gcagttggtg ttttccaaag gtccaggaaa atgcctcatc aagatggcct
     8761 gtgatgtcat tgcgtccttc cggaggcgta tttccagtgg actcaaaacc gttgatctgg
     8821 atatttggcg cttgtgaata gggagagttt gttacgaagc ccacgctgcc tacatcgaac
     8881 ccggtctttg catcgctgaa tgtctggttg aagtaattca ccccaatcgt gacctggttg
     8941 gtcatgcgcg gcgaaatgac ccagttatgt gcaaccgagt agttatatac atgaatcggg
     9001 ccgacctcga agtaccaggg atttacgtca gtactgccga ctggtgcagt ctggttgcct
     9061 tgtccaagaa atgcgcgcgc agacaggctg tggcgatcat tgaatgtgtg gtccagcttc
     9121 attacgccgt tgtagctgta accaaattcc ggagcagcgg aagagaagtt attcggactg
     9181 gcaggaacag atgaatttac caggtctgct ggccacaagt aactcaataa tgcatttgtg
     9241 gcggaattaa cagggacatt gtattgctgg agcaaggcct gtgccttctg ctgataaagc
     9301 actcccggtt ccgtgcctgc atttccagta gcgatattga atttctgctt ctcaaagtta
     9361 gcaaaccaga aagtgtggtc tttccagaat ggacctccaa gcgaggcgcc ccattgcaca
     9421 ttttcaagcg gcggatttcc ctgcacgaat ggggaaacaa ctgccagtgc ctggttgcgc
     9481 tcgtagtaat aagcagttcc atgcaattgg tttgtgccgg atttggtgac aagattgacg
     9541 gtgccgcctg gattgcgtcc tgtctctggg gctgattgtg tctgcaggct gaattctgca
     9601 atggaatcaa tcggcagggt cacgccggca atgttttcta caccgccctg gttaacggca
     9661 ggaatgttgt gccaccagtc attattgtcg gcaccgtcaa tctgccagtt gacctgattg
     9721 gcgcgggttc cattcaccga gccatagccg ccgagagagt atcctgcaaa accgggagca
     9781 aggccgatca tctgcgtaaa atcacggccg ttcagtggaa tgtcctgtac ggtcttgtct
     9841 ggaatcaccg tggtctgggt ggtagttgtc gtgtcaaggg caagccccgc cgccgatact
     9901 tccacagtgg tggcttcaga agcgacgggc agcttcatgg gcagggtgta gattgtccct
     9961 gcggttacgg taactttgcc gacctttaat ttctgaaatc cgctcgccga tgcagtcact
    10021 gtgtagctgc cgagaggcag gtcctgaaaa gaaaactcgc ccgcgctcga tgtgatcgtt
    10081 gtatgcgcga cctgcgtgtc gtctgcggtc gcaactacct gcgctccgct gatgaccgcg
    10141 ccggcctggt cagttacaac accattgatt ccgccgcgaa atgtctgcgc atctatcgcg
    10201 cggcccagcc ataagaaaac tacaaacaga aatgcgactg caatcgtttg aaatgaaata
    10261 cggcgactcg ggagtagcat catcacgacc tccaaaggat gaaattgcga aaagactgtt
    10321 tctttctgcg gtaggtgtgc tcaactgaag gacgcgcaaa gcagcgttgt ctcggaagac
    10381 ttgggatacc gcgatggatg aaacgtctga attggatcca tgagcaaact tagtccgtgt
    10441 aaattcgtcg tttcaacagg aaatgtggag tatttgctaa attaatccgc atttataagg
    10501 acgtttatgc agcaaggaat tttatgagta tggaaagtga ggtccgacaa gcggggcagt
    10561 gtataagcat caataagtta gaagaaaaac gagtgcgggc cgataaacaa ttgccaggga
    10621 ggcttatatc aaatgattat gcggttttgc cggaacagga actttgtaaa gagcgcttgc
    10681 aagtgagtac atacttcatg atgctttaca aataacgagg caacggttgc ggtgcagccg
    10741 tcgccttatt tcatgatctt ctcttagggg gactctgagc aaccggcaga ttggatgaat
    10801 gcgggaaggg cgagcggatt ttgaggatga tggcgcgctg aagttcgtta agggcctgtt
    10861 cacactatct gggcggatta gcgtccggga actgaattcc tttcctcttc gtacctttca
    10921 tcgaggccaa agatgagaga caacaaaatg aaagccggat tcgccgtctg cgcgctttgg
    10981 ataagcggcg gacttctttt tgcacaggac gcgacctcgc gacagaagct ggatctgccg
    11041 aaggcgccga cgttggtcat ggtcaaaaca cttccggaca aaggaacgct cgtcatgcaa
    11101 gtaaatgtgg gcggggtgaa catcgtccgc agcgacatgg agaaaaccat ccggctcacg
    11161 attgatccaa aggctttcta tgacgatgca acgcagatta cgcagacgcg cttcatccac
    11221 cgtgttccca cgattgatcc aaaggctttc tatgacgatg caaccgtaaa gtcatgggtg
    11281 cggcggtttg atgtggccgg ggaccgcgcc tggattgatt tgaaattgcc ggaccacggc
    11341 aacaatggga atcatcatca ctccagcccc gaaatcacga tagcggtccc tgcccaaacc
    11401 gacctgaagc tcgaattagg agtcggccag ttgacagtaa agggagtgga aggcaacaaa
    11461 gaactgcatg tcgggatcgg agagctggac gttggcttat ccgatgcctc gcaataccac
    11521 gagattcaga ccagctcaaa actgggcgaa gccgacgacg atacctcaaa ccagcattcc
    11581 ggcggattct tcccaaagac ccaccatacg tctgtccagg ggccgtataa gctgcacgca
    11641 accgttggaa ttgggcaggt gaatgttgcg aggaacgcaa acgaacgctg aggcccgcag
    11701 aaatggtgcc tgctcgggtg gagtgttgtt gtggtttcca cccttcgcgg aaacggagga
    11761 cacctgaaat cctgtgaagc gcgtttcaga caggttcgac gggcagatag cggcgtcccg
    11821 taatttcata cttgcctgag aggacccgtg cgatcgcttc cgtataggcg atgtgctcct
    11881 gctcgaggat gcgggccgac agggtttctg ctgtgtcttc atcgagcacc gagacggtct
    11941 tctgcaagac gatcacgccg tgatcaagct gctcatccac aaaatgcacg gtacagccgg
    12001 agacctttac tccatattcg agggcttgct gctgggcgtc gagtccggga aaggcgggca
    12061 gcagcgatgg gtggatgttc agaatgcgct gcgggaaggc gcgcacaaat tcaggagaga
    12121 gcaggcgcat gtagccggcc aggcagacca ggtccacatg gtgctcgcgc aggcaggcga
    12181 tgatttcagc gtcatgctcg gagcgcctgc gcccgcgcgc ttcaatcacg tgcgtggaca
    12241 ggccgcgttc gcgggcggcg gcgattccgg gtgcatcggc cttatttgag ataacaacgg
    12301 cgatttccgc gccctgcagt ttcccgcttg taatcgcatc cgctatggcg atgaagtttg
    12361 agccgcgtcc ggaaagcaga ataccgaggc gcttcggcgc gctcattgat aaaccacctt
    12421 gcggtcgccc ttgaccgtgc ggccaatcac atagaacttt tcgttcgcgc gctccagcgt
    12481 ggacttcacg cgcttgaact tgtccgccgg aacggcgcag atgaggccga tccccatatt
    12541 aaaggtgcgc agcatctcgt cttgtggcac attcccgagg tcgcgcaaat gttcaaagat
    12601 gggcagaaca ggccaactgc ccagttctac gacagcggag atgttttttg gcagaatgcg
    12661 cggaaggttt tccgtaatgc caccgccggt gatgtgcgcc atgcctcgca catactcgct
    12721 tgccgtgagt tttttgatca cgttgaggta gctgcggtgg atcttcatca gggccgctcc
    12781 ggccttttct ttaagcgcat tcacgtattg ctcggctttg tatccggcaa cctcaaagaa
    12841 gagcttacgg gcgagcgagt agccgttcgt gtgcagtccg gtggagggca agccaatgag
    12901 aacgtctccc ggttgaatat gggctccggt gatgatcctg tctttttcga caataccaac
    12961 aataaatcca gccagatcat actcgccatc ggcataaaaa ccaggcatct gggcggtttc
    13021 tccgccaatc agcgcgcagc cattggcgcg gcaggcgtcg gccaggccgc tgacgatttt
    13081 ttcagtcaca gcgccatcca gcttaccggt ggccaggtaa tccaggaaaa acaaaggagt
    13141 tgcgccctgg acggcaatat cattcacgca atgattgacc aggtcggccc cgacagtgtg
    13201 atggatgccc agttcaaagg ccactttcag cttggtgccc accccatcag cgcttgaaac
    13261 cagcacaggc tgctgatatt tggccgtatc cagcttaaag aggccgccaa aaccgccaat
    13321 ttctcctaaa acattgcggg taaaggtctt ttgggcaaga tacttgatgc gctgcttggc
    13381 ccggtcaccg ctggtgatgt ccaccccggc atcggcatag gtcacactgg aaatctgttc
    13441 tggcaatggt ctttccgctt caggaattag gttggaaaag ccagtttagc acctgcagag
    13501 cccggaaaat ctctcatagg agactttgca ttttgcaata aattttcaaa tttgcaatat
    13561 aaaaaagctt cactgaaggg aaatctggag ctaggctagt cgcaagcgca atttcttgcg
    13621 cattggagat tactgtgaaa aatgtgactc gtcttttcct ccccgcgctc ttggtgtgcc
    13681 tgtctgcctc ggcctttgca gcgaaaacaa cctccggccc tggggaccca agtcctatag
    13741 gacacgacag cttcacattc ggccctggag acccaagccc catcgggcat gattttgctg
    13801 cgtatggccc gggcgatccc agtcccattg gacacgatag catcgcgtcc ggtcctggag
    13861 atccgagtcc catagggcac tgacgctctg agcggttttt gaaaaattca gattgcaggc
    13921 catgtaggga aacagcgtaa gatgcggcta tgcatttgaa cctgctggat gacattctct
    13981 gggcggcaag ttttttcgga aatgttgtcc tgctggctgt tttgctgatc aagcgcagat
    14041 ggaaggagtt tcctgtcttt accattctga ttgctgattt cgcgattgag acacctgcct
    14101 tgttcctgat ttaccggtac agttcactgc actggtatgc aattatttac tggacaacct
    14161 atactctgga ctttgttttg caggtggcgc tggtatttga aatggcgcgg attgtgcttc
    14221 ggccgaccgg cacctgggtc cgggatgcgc gttccacatt tctgttgtgc ggggtggcag
    14281 gggccgcgat tgccctggtc ctgacatata tcgtgcatcc ggttgctcct tcatcgctgg
    14341 tggcatgggg agtgcgcgcg taccttttca gcagtttatt gttctgcgaa ttattttttg
    14401 ccatgatgct ggccgcccag cgtctggggc tggtgtggcg caaccatgtg atgggcctgg
    14461 ggcaggggct gactgcctgg tcgctcgcca cgatgctggt gcatgcggca catacttatt
    14521 ttggagcggt gaataaatac gtcttcaaca cgctggagca tgtccgcatc atcgtatata
    14581 tcagtgcaac gatttactgg gtgattacct tctggctgcc cgagcctgag cggcggccac
    14641 tttctccgga aatgcagaaa tatctggttg ccttacacga caaagtgcaa tacgatgctt
    14701 cacaggttag cggtgcccaa aatccccgtt gagagctttc cgtgattcca attcttctgc
    14761 tcctcacgtt cgtcgttcta ctggctgccg gactggtctg gatgcagatc agcaaccatc
    14821 gcctccactc cctgtcctgg gaagaccttg tgagtcaatt acagcccgta cccgcgcggg
    14881 gactggagat tgtggcgctc gattacatgg agccacgacc taaccagttg aagctcgaac
    14941 cggaccatct gtgggcattt atcggcggca tggaaggtct gcacggaatg cgcaggaacg
    15001 ccaatctgct gatagctctg gctgtccatg tccagcgctg gaatttcgag gaaggtgtca
    15061 ttgtcacaga acgtatgcgc caggacgctg ttcagcttaa gcgcgcgctg tttcgtatcc
    15121 ggatagaaat gcttctgcgg cgcacgcaat tgcggacgcc gttctatatt cagcaggccg
    15181 catcatccta ttatctgatg acgaagcgcc tgcttgccct gtatgagacc agccacgcag
    15241 gtctgctgcc ggtcctcaaa gcggccatat aagggcttcg cgccgcttca ttcataaacc
    15301 ctatactgaa gggaaatgcg aacacttgac cgctcccttg ccctccaggc ccgcgcagaa
    15361 aagctctttc ccggtggtgt agattcacct gtacgcgcat ttcgcgccgt cggcgggaat
    15421 ccgcccttcc ttgtgaaggg agaaggcgct catttgtggg acgccgacgg caaccgttac
    15481 ctcgattatt tcggctcgtg ggggccaatg attctgggtc acgcctttcc tccagctgtt
    15541 gaagcgatcc ggaaagcagc ggcggaaagc gcaagtttcg gcgcgtctac tccggcggaa
    15601 gcagacttgg cggaagccgt cgcgcaggcc tttccctcga tggagaagat gcgcttcgtc
    15661 agctccggca ctgaagcgac catgacggcc atccgcttgg cgcgcgcgtt caccgggcgg
    15721 aaatacgttg tgaaattcga gggctgctat catgggcact ctgatgggct tctggtgaag
    15781 gccggttcag gcgtggcgac gtttgggatt cccggctcgg caggtgttcc ggaagagatt
    15841 gcgcatttca cgctggcgct tccgttcaac gatgttcccg cagtcgaagc ggcatttgca
    15901 aaacatcgtg cagagattgc ctgcatcatc gttgagcctg tcgtcggcaa catgggctgc
    15961 gttcctccgg cagaaggctt tttgcagtcc ctccacgaga tcaaacagcg ggaaggcgcg
    16021 ctgctcatct tcgatgaagt gattaccggc ttccgcctcg catttggcgg cgcgcaggag
    16081 gtgtatggca tccggcccga cctgacgacc ctggggaaaa ttctcggtgg gggcctgccg
    16141 tgcggcgcat ttggcggacg caccgaaatt atggacatgc tcgccccgct tggacctgtt
    16201 taccaggcag gaacgctcag tggaaatccg ctggccatgg ccgcgggcat agcaacgctg
    16261 agttatctga aggagcatcg cggcgaagtt tatccacagc tggaaaagct tgctgctgcc
    16321 gtcgccaatg gagtggcggc tgtagccgcg aaagcagggg tcccagtggc ggtcaaccgc
    16381 gtcggttcca tgttcacgtg gttctttact cctgagcggg taacggattt cgcttccgcc
    16441 tcaagttctg acacggctgc ttatgcgcgc tttcatcgcg ccatgctgga gggcggagtg
    16501 tggctgcccc catcgcagtt tgaagcagca tttgtaagca ccgcgcacac aatggacgat
    16561 gtagatacca ccattgccgc tgcgcgcgag gcacttgctg tctggaccag ataagtccgg
    16621 cggcgcgtca atgctggcat gtccggcgag ttatttctgg catctggggc agtagtgtgt
    16681 gccccggcct gccagaacca gcctccggat ggccgtcccg cacaccagac agggtcttcc
    16741 gcctcgcaaa tagaccttat gttcaagctg aaagaagcct ctagcaccgt tcgcgtctac
    16801 gtagtcggaa acagaggagc cgcccagacg gattgcatct ttgagcacag tttgcagggc
    16861 cgagtgaagg agattcaact ccttcttttt cagcgatttg gccatgcgtg tgggacgaat
    16921 tccggcgcgg aagaggcctt catcggcata aatattgccc acgccgtgca acagcttctg
    16981 gtttaaaagc gcagccttaa tcgaggtctt gcgcccacga aataattctg caaattcttc
    17041 aaaagaaatg gttaaaggct cgctccccgg gccggtgaag gccgttctct ctcgaatatc
    17101 cagccgtccg aatcgccgag ggtcaataaa gcgcagctcg cggcctgagc tgaaatggag
    17161 gaccgcatga gtatgatttg cgacagaatc agcggaattg caggctaaaa gccgcccagt
    17221 catcccaaga tggactatcc attgcgtgtc ttgggaaaga tcgaagacga tatgtttgcc
    17281 cacccggtag acgcgctcaa tttttttgcc cgtaagggcg cgggccatct ttcgggggct
    17341 ggttttgaat ggctccggtt tggagctgaa ccagaccgac tcgacggtct cgcctcgcac
    17401 gcgttcatgg acgccacggg cgacggtttc gacttccgga agttcgggca tgagggtccc
    17461 tgcttttatt ttctcaggga gcagaacatt ttccattctg ctacactcaa tcgtttccta
    17521 tgtctgacgc tactccgctg acgcaactga atgaagatga gcagctcttc cgctccaccg
    17581 tgcgcgaatt cgcgcagcag accattggac cgcttgtgcg gcagatggac gaggagcagc
    17641 actttgctgc ggagcttttg ccgcaactgt tcagcctggg gctgatggga atcgaggtgc
    17701 cggtagagta tggggggagc ggcggcacgt tttttgaggc cgtgctcgct gtggaagaaa
    17761 tctctgccgt agatcctgcc gttggcgtcc tggtagacgt gcagaacaca ctctgcatca
    17821 atgcgctttt gcgctgggga acagaggcgc agaagaaaac atatctgcca aagatggcta
    17881 cggacacggt aggcgcatat gcgctttctg aggcaggctc cggttctgat gcgttcgcat
    17941 tacagacgcg cgctgagttg cgcgacggtc actacgttct gaatggccag aagttgtgga
    18001 tcacaaacgc gaaagaggcc gggctgttta tcgtcttcgc tacactcgat gcttccgccg
    18061 gatacaaagg cattacagcg tttttagtcg aaaaggacac gccgggattt tctgtgggga
    18121 agaaggaaga caaactgggc attcgcgcct cttctacctg tgagttaatt ctggatgact
    18181 gccgcgtgcc tcaggagaat gttctgggcg agccgggcaa gggatacaag attgccattg
    18241 agacgctgaa tgaaggccgt atcggcattg gcgcgcaaat gctgggtctg gcgcagggcg
    18301 catggaactg cgcggcaaaa tacgcgcagg agcgcaggca attcggcaag ccgattgctg
    18361 aattccaggc cgtgcaattt gcgctggcag agatggcaac caaaattgag gccacgcggc
    18421 tgctggtcta caacgctgcg cggctcaaag acgcaaaaca gagctatatc cgcgaagctg
    18481 cgatggcgaa gtatttctca tcccaggtgg cagagcgcgt cgccagccag gccgttgaga
    18541 tttatggcgg ttatggattt gtgaaggact atccggtaga gaagttttac cgcgacgcca
    18601 agattggcaa gatttacgaa ggcacatcaa acatgcagct cgccacaatt gggaagctgg
    18661 tgctgggtgg aagaggctga gggactttgc ctcctcgatc cctcgaataa gcgtcggcct
    18721 ggacaacaaa ttcagaacaa aagcaagaca gttaaaagca atttccggaa cggcaattca
    18781 tgggtgtgta cgctgcgcgc tgcagcctgt accctgtatt tagtagccac ctttcatgct
    18841 gggcctttat cagagactca ttctcggatt tctgcttttg attgcgctgg ttggcggact
    18901 gggcatgctg gtgcgccact cctttgtgca tctttctgca ttggacgagc agcagcagac
    18961 tgaggattcc gctgtcagcg cgctcgcgca gatacaggcg tcacttgcgc gcgaacaggt
    19021 gctgacagga agattggctg tgaacccggc ccgcccggtc tacgagcaac tgatggcgca
    19081 ggccgaagtc accgagcaac ggttcgttgc cgctgtgcgc acacaggcgc ctgcactggg
    19141 acaactcgag caaaagcaca ggcaggtggt ggaaagactg cgcggcaacc tgaataatcc
    19201 gaatgcatgg cgcgacctga cggccaatgt attccgtccg caactggaca cgattgacga
    19261 agggctttct gacaacctgc gcgatctctt tgtccggcgc gcttctctga tggccgatct
    19321 ggatcacgaa cgctcgatgc tgcgggcgcg cctgattgcc atttccgggt tcagtctgtt
    19381 tgtcgccctg gccatttccg ccattactac gttcctggtc atccggccca ttcgcgcaac
    19441 gacccggaca gcgcagcaga ttggccaggg agacctgcat cgccgcattg aatggacgat
    19501 gaatgacgac ctgggcgcgc tggcaactga gatcaaccgt atggctattc gcctgcgcga
    19561 tttacgcgag accgagtcgg gtcgccgcca gatggaacag caactcagcg acgccgtcat
    19621 caactccatc ttcgagccgg tcatcgtcac cgacgcgaag ggccatgtgc tcaagctgaa
    19681 ccaggcggcg acggagctgc tgggcaagtc tgaccgcatg gcgctggcga acactccggg
    19741 cggggaaaaa attctgaacg ccgtgcgcaa tgccgtctcc atgcaacgcg cgacgacggg
    19801 agaaggcgaa gcggcgctgt tgccgatgcg catcggcagt gccgaacgca gctaccgcct
    19861 gcgcacagca cccatgcgtg acgcggacgg aaagctgctg ggcgcggtga ccgtgctgga
    19921 agacgtgacg gagatgcgcg acattgaccg tttcaagacg cgctttctct ctgttgcatc
    19981 gcagaagctg cgcgatcccc tgcagcgtct gcggctcgcg ctttatgccc ttacgcgggg
    20041 acatgccgga gagctgcgcc cgctccagtt tgaaattctg gaagacgcaa cgcaggaaac
    20101 ggagcggctc gatgatctga tggccgacct gattgaagtg gccgagcttg acacgggccg
    20161 ccgccagatg aagatcgagc ggctgcgccc gatagacatt ctggaagatg catgtcatcg
    20221 ctttcgggag caggcgcgcg agaagaagat tgaaatcgag gttgaagcat ttgcagacct
    20281 ctcctatgtg aacgcagacc gccgcgcgct gcgcagcatt ctggacaacc tgctttccaa
    20341 cgcactgcgc tacacgccgg aaggaggaca ggtgcgcctg aaggcatctg agctgaaaga
    20401 ccgcattcag tttgtggtac aggactccgg acgcgggatt gaggccgaac gcctgcccac
    20461 gatttttggc cgttttgccg gcacttccag cgatggcacc ggtctggggc ttgcactggt
    20521 acgccgtctg gtggagacac tgggcgggca ggtttccgtg gaaagccgtc tcgggcaagg
    20581 caccacattc agttttactt tgcccattgc cacacctgct ccggcccggc atgaagtgga
    20641 ggtgggctga agatggacaa aggtaaactg cgcgtctatc ttggcgccgc tcccggagta
    20701 ggcaagacct accgtatgct ggaagacgcc cacctgctca ggcagcaggg cgtggatgtc
    20761 gtcattggcc tgattgagac gcatgggcgt aaagatacag cggcgcttat cggtgatctc
    20821 gaagtcatcc cgctcaagga ggtcccgtac cgcacggtca ctctgcgcga gatggacatc
    20881 gaggcaatcc ttgcgcgcca tccgcatacg tgcattgtgg acgagctggc gcactcaaac
    20941 gtcccgggct cgcgcaaccg caagcggtat gaggatgttc tggaactgat ggacgccgga
    21001 atcaacgtaa tgacggccgt caacatccag catcttgaga cgctgaatga cgcagtttca
    21061 cgctcggcca gcacccaaat ccgcgagact gttccggaca gctttctgaa gcgggccgat
    21121 gaagtggtga acgtagacgt taccattgaa gagctgcgcg ggcgcctgcg cgaaggcaag
    21181 atctacgctc cggaaaaaat agagcaggcg ctcgccaact tcttccgcaa aggcaacctg
    21241 aacatgctgc gcgagttggc gctgcgcacg acagcggaac aggtaagcac ggcggcctca
    21301 gaataccggc gcatccaggg gctggagcag gcgccgattc cggaaaaagt gatggtctgc
    21361 atctccaccc gtcccggtac agaaagactg attcgtctgg gagcgcgcat tgcaggacgc
    21421 cttgctacga actggtacgt ggtgtatgta gataccgaga aggaccacca cgaagacccg
    21481 gaagcgcggc accgcctgga agagtaccag cgcatgtcgc gtgatctggg cgcaaaagtc
    21541 atcgtgctca aagacaagaa tgtggccgac gccctgatta acttcacgaa aaaagaaaac
    21601 atctcgcatg ttatctttgg gcaatcggcg cgctcgcggt ttgaaattct gctgcacggc
    21661 tcggtcatca accgcttcct ggccgaagtg cgcgacacga ctgtacaggt ggtgccgatg
    21721 agcagagaga agaagtaaaa ctgtcccgct ccgctgcgcg aagttgtgcc tgcatggggt
    21781 gtttattctt tcctcaggac ggcgctgcgg tgttcgtccg cagtgatggc accctcaaac
    21841 tttttcagag catcgtactc gctggcggga agctctaact gccgtacaac cagctcgcgc
    21901 gaatagcgca attcattctt gctgactttg acctcgctct gataactggc aaatcccgca
    21961 tctattttta ccggctcggg cgtttcatcc accgtatagc cgctgggcaa agtgatggtg
    22021 aatgaatcgc gccacgtgcc ttctctttcc agattgaccg gatattttct ttccttgtcg
    22081 tcaagcgcct cggccacaga cccaagcacg cgcggacgca ccagaagaag gtctccggca
    22141 tgttttgcgt atccctgcgc tgtgaattga taatgcagga caaagtcttt ctcaagattt
    22201 tgggcgttct ctgcattttc tgatgcaagc gcaaagtcag aaaagtcctg ctggagccgt
    22261 cgttcaagat attgctggcg ctctttgccg gcgttcagcg caaagtaatg ccgttccgcc
    22321 gccgcggacg cacccgtgcg cgtttctagc acagagcctt caagagtgcc gtcctctttc
    22381 agtgtgaact tgcctgtgtg ttctgttacg tctgcgtccg gaggtacgac cggaagctgg
    22441 accacctggc tgtgcgctcc attcaccagc gttccgtaac tgccctggag ataggtgggc
    22501 agcagcccca aaggcacgta ttcattggtt ggatcgaaga taagaaagtg ttcgcccgcg
    22561 cgggtcttta ccaccgtctt caggcgcgca tcattgtagc cctgcggcag ttcgatggcg
    22621 gcgatggcgt ggtttccatc gatcgagggg acatcggcat ccacaaagcc gcggcgtgtg
    22681 tctaccaaaa cgtaggtggc atgcactcca atggcgcgca gcatggcgat gagcaatgtc
    22741 gctttgtctt tgcaatcgcc atagcggttg cgataaatgt cggccgccgg atgcggctgg
    22801 tatccgccga ttccaatctc gatgccgacg taacggatgt cccgctgcag gaactcagct
    22861 attttttcca cccgggcggt gaagtcgccc tctccgccca caagcgactg cgctttggcc
    22921 gcgatctcac tcgtggtttc actgcgcgat gtggcgagat cgtcatacca ctggccaatg
    22981 ctggtccaga gctggtcccc ctgcggaata tcgccggcgg cataatgcac gaccatgcgg
    23041 ccttcgagcg caccttccgg tggaggcagc ggcacatctt ccagatctac tgccggaaca
    23101 tgggccagct cccatctcca atggtttggc gcaatttcct gtggctgcac agcggcataa
    23161 tggtgccaga ctgcgcgctg cttccatcct gtaggaagat ccacctcaaa cactgcgcgg
    23221 gccacgggga tgtctgtctg aaagtcccat gagctttcct tcatatattg cggcagcagg
    23281 ttttctgact cagatgcgac gacgcctccc gggtctgctc ccggaggcgt ggccactttt
    23341 gcacgatagt ccacatagag catgccaccg ccgtctgcgc cgacttctct gatttcgttg
    23401 tccctgactg tatatggatg gccatcgggc gcgatgctcc atgcatggaa cgagagcagc
    23461 tttttgccca tgccgtttcc ggcaaagagt tccccatatt cacgcccctg agggcgcagc
    23521 agtttgatga cttctctgcg gcggattctc gct
//
LOCUS       DASRBV010000053         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_255826, whole genome shotgun sequence.
ACCESSION   DASRBV010000053 DASRBV010000000
VERSION     DASRBV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255826"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFA02_02300"
     CDS             complement(<1..1481)
                     /locus_tag="VFA02_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HZU08707.1"
                     /translation="MPPVAESQKKLNLKYLEDRWNESEAARMDEPELLRYRSNLLGSD
                     LRITNFGGGNTSSKINQADPLDGSIRRVLWVKGSGGDLGSIRRNGFATLYLDKLLGLE
                     KIYRGAEFEDEMVDYYPLCTFGINPVAASIDTPLHGFLPYAHVDHLHPDCGIALAAAA
                     NGREKMEQFNREYGHRLIWLMWQRPGFELAMMLKRAVVENPGCDGVILGGHGLFTWGD
                     TQRECYANTISIIDELTDFIEKHIQKKGAAIFGGQKRKVLDNRRELALEILPFLRGRV
                     SQQKRLIGTYCDLPEVLEFVNAVDAAKLAYLGTSCPDHFVRTKIRPLFVDWEPASGAD
                     VLKKQIESGLEQYRKDYASYYEDHRDPSSPAMRDPNPTVVLIPGVGMFSFGKNKPESR
                     ITGEFYVNAIHVMKGAAALEEGWVTGVVPQCGSAALARDFRVEDNYVALPASEAFRIE
                     YWKLEEAKLRRMPPEKELSRQIALVVGGGSGIGREVALLAAERG"
     gene            complement(1561..2751)
                     /locus_tag="VFA02_02305"
     CDS             complement(1561..2751)
                     /locus_tag="VFA02_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU08708.1"
                     /translation="MRVEDRFERVLGQLDSFVIEIPSWGFANTGTRFGKFLQPGAAGT
                     LEEKFADAAQVHNLTGATPSLALHVLWDLPGGVKDAPKVNQLVSRYGVRAGSINPNLF
                     QDQEYKFGSICNPDAGIRRKAVDHLLDSVEIAKALGSQDVSLWVADGSNYPGAQSIRR
                     RIEWLEEALRTAHDALDEGQRLLIEYKPFEPAFYFTDIADWGMSASLAGAVGPKAFVL
                     VDTGHHYQSQNIEQIVAWLLHLRLLGGFHFNDRRYADDDLTIGSIDPYQVFRIFHEIL
                     NERDKNVTDAVAYMIDQSHNIKGKLEAMVQTVATAQELYAKALLVDRERLAELQAAAK
                     TVEAEECLRDAFWTDVRPLLKTWRERRGLPADPLKALRESGYVEKIERERGARNAALI
                     SSYA"
     gene            2888..4366
                     /locus_tag="VFA02_02310"
     CDS             2888..4366
                     /locus_tag="VFA02_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HZU08709.1"
                     /translation="MTRNYLLAAPLLVAAVACAQQPQSISVDWSKTILVSRSTPTLQV
                     VVNPMLRRGSPIHQQSFAALKALGADYVRYVPWLPYPKLAVAELEPGSWDFSLIDPMT
                     TDFLAATEGHSTVMNFSTMPAWLFRTDKPVTWPDDPNQVTWDYTQGTELKDPSGKDLG
                     DYYARLVSWYTKAGFTDENGKEHHSGYHYSFPYWEVLNEPDFEHQTTPEQYTQRYDTI
                     VSAIHQVSPQTKFVGLALAMADRPDMYEYFLNHAHHKPGIPLDFISYHFYASPTDGHD
                     PNVWQYQFFDQADRFLSEVKFIEAIRKRLSPETKTDIDELGVILPGDGEENALRKALP
                     FTTPEIYWNAAGAMYAYLFIGLAKQGIDVIGESQLVGYPSQFPSVSMMDWRSGRPNAR
                     YWVLKLIKDNFHPGDKLTETQIVHDRHEAAVQAFLTPEGKKLLLINKRNQPVEITLPQ
                     EAANAKLSTVDETTGEGAAGATQANGTAVMLAPYAVTVVSWP"
     gene            complement(4378..>5229)
                     /locus_tag="VFA02_02315"
     CDS             complement(4378..>5229)
                     /locus_tag="VFA02_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU08710.1"
                     /translation="FGITFAISFTALLVWEWFAKNPIMELRLFKNRNFATCCFLMLFT
                     GGLLNATTVLQPQFLQAQLGYTATIAGLSLSAGGVVLLFMMPLAGQAVSRFPARNIIL
                     FGFTCFAFSYYFTATRINLGLSFGMASWLRIVQIFAIPFVFISVTTAAYFGMPVEKNN
                     QVSGLINFVRNIGGSILISLTNAGITELGQFHQNQMMKHLTPSSANLQGQISTLGSVF
                     TKTAGSANANYLAQGQIYNQLQQQSQTLAYKDMYFILCAASLIMIPLSLLLRKNKPGG
                     SGEIAVH"
BASE COUNT         1225 a         1600 c         1255 g         1149 t
ORIGIN      
        1 ccgcgttccg ccgcaagcag cgctacttct ctgccaatac cgcttccgcc gccaaccacc
       61 agcgcaatct gacggctcaa ttccttctcc ggaggcatac gccgcagctt ggcctcttcg
      121 agcttccagt attcaatgcg gaaggcctcc gaggcaggca gtgctacata gttgtcttcc
      181 accctgaagt ctctggccag cgccgcagaa ccacactgtg ggaccacgcc cgtaacccag
      241 ccttcctcca gcgccgccgc gcccttcatc acatgaatgg cgttcacata aaactcaccc
      301 gtaatgcgcg actccggctt gttcttgcca aagctaaaca tgccgacgcc gggaataagc
      361 acgaccgtcg ggttcggatc gcgcattgct ggagaggagg ggtcgcgatg gtcctcgtag
      421 tagctcgcgt agtctttgcg atactgctcc aggccgcttt caatctgctt cttcagaaca
      481 tccgcaccgc tcgccggttc ccagtccaca aacagaggcc gaatcttggt gcggacaaag
      541 tggtccggac agcttgttcc caggtacgcc agctttgccg catccactgc attgacaaac
      601 tccagcacct cagggaggtc gcagtatgtc ccaatcagcc gcttctgctg cgagacgcgt
      661 ccgcgcagga aggggagaat ttccagcgcc agctcgcggc gattgtcgag tacctttctc
      721 ttttgtccgc cgaaaatcgc agcgcccttc ttctgaatgt gcttctcaat aaagtccgtc
      781 agctcatcaa taatggaaat cgtgttcgca tagcattcgc gctgcgtatc gccccaggta
      841 aacagcccat ggccgcccag aatgacgccg tcgcaacccg gattctccac aaccgcgcgc
      901 ttcagcatca tggccaattc aaaccctggc cgctgccaca tcagccagat gagcctgtga
      961 ccgtactcgc ggttgaactg ctccatcttc tcgcgcccgt tggccgccgc cgccagcgca
     1021 atgccgcaat ccggatgcag atggtccaca tgcgcatacg gcagaaagcc gtgcaatgga
     1081 gtatcaatcg aggccgccac cggattgata ccaaaagtgc acagcggata gtagtccacc
     1141 atctcgtctt caaattccgc gccgcgatag attttctcca gccccagcag cttgtccaga
     1201 tacagcgtgg caaaaccatt gcgccggatg ctgcccagat caccaccgct tcccttcacc
     1261 cacagcactc gcctgatgct gccgtcgagc ggatccgctt ggttgatctt cgagctggta
     1321 ttcccgccgc caaagttggt aatgcgcagg tccgatccaa gcaggttcga gcggtaacgc
     1381 agaagctccg gctcgtccat tctggccgcc tcgctctcat tccagcggtc ttccagatat
     1441 ttcaggttga gttttttttg agattcggcg acgggaggca tagcaaactt ccttttctgg
     1501 tctcagaagc aattcagacc gattatatct atatagaata tatttaccaa tagaaattat
     1561 tcacgcatag ctactgataa gcgcggcatt cctcgccccg cgctcgcgct caattttctc
     1621 tacatatccg ctttcccgca gggccttcag cggatcagca ggcaatccgc gccgctcgcg
     1681 ccacgtcttc agcagcggac gcacatccgt ccagaacgca tcgcgcagac actcttctgc
     1741 ctcgaccgtc tttgctgctg cctgtaattc cgcaagccgc tcgcggtcca ccaggagcgc
     1801 cttcgcatac agttcctgcg ccgtggcgac agtctgcacc atcgcctcaa gctttccttt
     1861 aatgttgtgg ctctgatcaa tcatataggc aactgcatca gttacatttt tgtcgcgctc
     1921 gttcaggatt tcgtggaaga tgcgaaacac ctggtacgga tcaatcgaac caatcgtcag
     1981 atcgtcatcg gcatagcggc ggtcgttgaa gtggaatccg ccgaggagcc gcagatgcag
     2041 cagccacgcc acaatctgct cgatattctg tgactgataa tgatgtccgg tgtctaccag
     2101 cacaaaggcc tttggcccca cggccccggc caaactcgcc gacatgcccc agtctgcaat
     2161 atcagtgaaa taaaaggccg gctcaaatgg cttatattca atcagcagac gttgaccttc
     2221 atccagtgca tcatgcgccg ttcgtaacgc ctcttccaac cactcaatgc gtcttctgat
     2281 gctctgcgct cccggataat tcgagccatc ggccacccac agcgacacat cctgtgaacc
     2341 caacgccttt gcaatctcga ccgaatccag cagatggtct accgccttac ggcggatacc
     2401 agcatccgga ttgcagatgg acccaaattt gtactcctgg tcctgaaaaa ggttcgggtt
     2461 gatggatccg gcgcgtaccc catagcggga cacgagctga ttcaccttcg gcgcatcctt
     2521 cactcccccg ggcaggtccc acagtacgtg aagcgccagc gatggagtcg ccccagtcag
     2581 gttatgcacc tgtgcggcgt cggcaaattt ctcttcgagc gttccagcgg ccccgggttg
     2641 caaaaacttc ccaaaacgcg tgcctgtatt ggcaaatccc caggaaggaa tctcaatcac
     2701 aaagctgtcc agctgcccca gcacccgctc aaagcggtct tctacgcgca tcgcatcctc
     2761 tttccagaac ctgaatcagg caaagcggag tgtagctcca tttgcatctc attttcaata
     2821 gaaattctat ttcatatttg tattctggcg tactatcatt gcgccgttgc cttttggggc
     2881 cattctaatg acgcgaaatt atcttcttgc ggcaccgctg cttgtggccg ctgttgcttg
     2941 tgcccaacag cctcaatcca tcagcgtgga ctggagcaag accattctcg tctccagatc
     3001 cacgccgacc ttgcaggtgg tggtcaatcc catgctccga cgaggttcac ctatccatca
     3061 acagtccttt gctgcattga aagcacttgg cgctgattat gttcgctatg ttccctggct
     3121 tccctatcca aaactagccg tggccgaact cgaaccaggc tcatgggact tctcgctgat
     3181 tgatccgatg acaacagact tccttgccgc tacggaaggc cattccaccg tcatgaattt
     3241 cagcaccatg cccgcatggc tcttcaggac agacaaacct gtcacctggc ccgacgatcc
     3301 caatcaggtc acatgggact acacgcaagg caccgaactg aaagacccgt cagggaaaga
     3361 tctgggcgat tattatgctc gtctcgtcag ctggtacacc aaggccggct ttactgacga
     3421 aaatggcaaa gagcaccatt ccggctatca ctacagcttt ccctattggg aggtcctgaa
     3481 cgagccggat ttcgagcacc agaccactcc tgaacagtac acgcaacgct atgacactat
     3541 tgtttcagcc atccatcaag tcagcccgca gacgaagttc gtcggccttg cgctggccat
     3601 ggcagaccgg ccggatatgt acgagtactt cctcaaccac gcacatcata agcccggaat
     3661 cccgctcgac ttcatctcct atcactttta cgcatcgccc accgatggcc acgaccccaa
     3721 tgtctggcaa tatcaatttt tcgaccaggc cgaccgcttt ctgtccgagg tcaagtttat
     3781 tgaagccatc cgcaagcggc tctctcccga aaccaaaaca gacatagacg agttgggagt
     3841 cattcttccc ggcgatggcg aagaaaatgc cttgcgaaag gccctcccct tcaccactcc
     3901 cgagatttac tggaatgccg cgggcgccat gtacgcctat ctcttcatcg gcctcgcaaa
     3961 acaaggcatt gacgtcattg gcgaatcgca gctcgtcggc tatccatcgc agtttcccag
     4021 tgtctccatg atggactggc gcagcggaag gccaaatgcg cgctactggg tgctgaagct
     4081 gatcaaagac aacttccatc ccggagacaa actcacggaa acacagattg ttcacgacag
     4141 gcatgaagca gccgtacagg cttttcttac gcctgaaggc aaaaaactgc tcctcattaa
     4201 caaacgtaac cagccggtcg aaatcacatt gccacaagag gctgcgaatg caaagctgtc
     4261 caccgtagac gaaacaacag gagaaggcgc agcgggggca actcaggcga atggtacagc
     4321 ggttatgctc gccccttatg cggtcaccgt cgtctcatgg ccataagcag ttgttcacta
     4381 gtggacggca atctcaccac tgccgccagg cttgtttttg cgcaacaaaa gactcagcgg
     4441 aatcatgatc agcgatgcgg cgcaaaggat aaaatacatg tccttatatg caagcgtctg
     4501 cgactgctgc tgtagctggt tgtagatctg cccttgcgcc agataattcg cattggccga
     4561 tccagcggtc ttcgtaaaaa cgctgcctaa tgtgctgatt tgtccctgaa gatttgcgct
     4621 ggacggagtc aggtgcttca tcatctggtt ctggtggaac tgccctaact ccgtaatgcc
     4681 tgcatttgta agagaaatca aaatacttcc gccaatattg cgcacaaagt taatcaggcc
     4741 agaaacctga ttgttcttct caacgggcat tccaaaatac gcggccgttg tgacggagat
     4801 aaacacgaac ggaatcgcga aaatctggac gattctaagc catgaagcca ttccgaaact
     4861 aaggccaagg ttgattcgcg ttgccgtgaa gtaatacgaa aaagcgaagc aggtaaatcc
     4921 gaacaggatg atatttcgcg ccgggaatcg gctgactgcc tggccggcaa gcggcatcat
     4981 gaagagcagg accacgcctc cagcggaaag cgacaggccg gcaatggtcg cggtgtatcc
     5041 aagctgggcc tgaagaaact gtggctgcaa tactgtcgtc gcgttcaaca gcccgccagt
     5101 aaacaacatc agaaagcagc atgtagcgaa gttcctgttc ttaaacaggc gcaactccat
     5161 gatggggttc tttgcaaacc actcccacac cagcaacgcc gtaaaggaaa tcgcaaaggt
     5221 aataccaaa
//
LOCUS       DASRBV010000054         9278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_662918, whole genome shotgun sequence.
ACCESSION   DASRBV010000054 DASRBV010000000
VERSION     DASRBV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662918"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFA02_02320"
     CDS             complement(<1..155)
                     /locus_tag="VFA02_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZU08711.1"
                     /translation="MATGMTKTALVRHLAEKLELTNKQAAAFLDLLAETAIKETKKNG
                     LFVIPGLG"
     gene            complement(632..1369)
                     /locus_tag="VFA02_02325"
     CDS             complement(632..1369)
                     /locus_tag="VFA02_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HZU08712.1"
                     /translation="MPKNSVVPANPYFNEQALKFLRGLRRNNNREWFQARREIYEREL
                     KRPMLAVIEQVTDAMADFAPAHVRPAEKSMMRIYRDTRFSADKTPYKTHVAAWWARLG
                     MEKTSGGGYYFHFSPAEVIVAAGIYMPQREQLFAIRMQLLEQHEEFRKLLKDKKLRKM
                     MDLDLDVRALSRPPKGFPADHPGIDLIKQQQWGVFARLPAETALKSTLVKEIVTRFRL
                     AAPIVDFLNRPFRHEVEKKRQYIFKLM"
     gene            complement(1383..3869)
                     /locus_tag="VFA02_02330"
     CDS             complement(1383..3869)
                     /locus_tag="VFA02_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HZU08713.1"
                     /translation="MPTATRYICIHGHFYQPPRENPWLEQVETQDSAAPFHDWNERIT
                     SECYAPNGASRIVNQKGEIIRILNNYAHMSFNFGPTLLSWLEQCAPRTYRMILEADHA
                     SQMKFSGHGSAMAQVYNHMIMPLASRRDKTTQIRWGIADFERRFGRRPEGMWLAETAV
                     DSETLELLAEHHIQFTVLAPHQCARVRPMGKDKDAPWMETSAETLDTKHPYIIRLKNK
                     KKIAVFFYDGPRSRAVAFEGLLNSGETFAARLMGGFTDEDAPQLVHIATDGESYGHHH
                     KQGEMALSYALKLIEQNPDIALTNYGEFLEKFPPQWEAQIAENTSWSCSHGIERWRSN
                     CGCNAGHPGWNQEWRAPLREALDWLRDAVAPLTEETARPLFKDVWAARDGYIHVVLDR
                     ADLNQFLARYAKRELNAEERITALKLMELQRHALLMFTSCGWFFDEISGIEASQVIAY
                     AARVLQLAAQLFGPRGAGLEEHFIEILRKAKSNLPQEGDGASVYLRRISTLKVGLEQV
                     AAHYAISSLFSNYPDEATLYCYTARRLAYEVITSGHSRLLLGQARIISQITEESQEAS
                     FAVVHFGDQNINAVVQRYDESQSEKYREFAEKARAAVTNADFPEVVRLFDRTFSGPSY
                     SIQSLFKDEQRRILNMILQPPISEVESSLIAIYEGHASLLHFLSRNGFPKPRALELAA
                     DFAVNAGLRRAIDSSPIDALQVRTWLRIADTDKIALDSGLLRYIVDLKMKRVMVALHA
                     APERMDLLDDALLTARTVSELPFELNLWQAQNIWYDTLRLSWNQDFSPEWKEKFHELG
                     AQMKILVDELVVEEEATNGHQQEKAESAVS"
     gene            complement(3960..4598)
                     /locus_tag="VFA02_02335"
     CDS             complement(3960..4598)
                     /locus_tag="VFA02_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08714.1"
                     /translation="MKTDKYGRPISWLIAAWFILALTASGLGIFQNPPSTPPIALGLA
                     VLVPVLIFLAWYGSSQDFRAFVLSLDLRLLTMLQSWRIAGFGFLALYTYKILPGSFAL
                     PAGWGDLFIGVTAPLIALKLADRPHRAAFILWQILGMLDLVTAIGSGVTTGFISHGPV
                     TMDPITALPLSLIPTFGVPFFFILHIISIAQTQQWQKADYASRKVPLSSTAI"
     gene            complement(4595..5071)
                     /locus_tag="VFA02_02340"
     CDS             complement(4595..5071)
                     /locus_tag="VFA02_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU08715.1"
                     /translation="MDAYTSISDMKEPVLPSLPCLCASLRRAARALTQCYEEALRPHG
                     LRATQLTVLQVLSAVGEVSQGRLGEILAMDSTTLTRTLDILYREGWISRKKGTDRRQW
                     RLGLSSSGKEKLQRTLPAWKKAQQTMARQLGDKHWHDIFQLTDEIATLARNQERLL"
     gene            5270..7090
                     /locus_tag="VFA02_02345"
     CDS             5270..7090
                     /locus_tag="VFA02_02345"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZU08716.1"
                     /translation="MKRILATTLLFATVFIGQTSFAAQPHTFKAENGQFTLDGKPFQV
                     ISGEMHYPRIPRAYWRDRLRMAKAMGLNTITTYVFWNVHEPRPGVYDFSGNNDVAEFI
                     REAQQEGLYVILRPGPYVCAEWDFGGYPAWLLKDHDMVVRSRDPKFLAAAQRWIKRLG
                     QELAPLQIGNGGPIILVQVENEYGSFDKDHDYMEDIHHMLVDAGFTKAQLYTADGPEQ
                     VPDGSLPELPAAINFGPGESQKGFATLKKLRPEGPFMNSEYWDGWFDHWGSKHAKTDA
                     QQQNSDLDWTLRQGYSISLYMFHGGTSFGWMNGANSNGKNYEPDVTSYDYDSPLDESG
                     RPTPKYFLFRDTIAKATGKTPPPVPPVAPPMEVPAFSLTQSASLWDNLPRPTHSEQPL
                     SMEDLGQAFGYILYRTTLSGPVTGDLVLDQLHDYAQIYVDGKLVGTLDRRLHQNSLPL
                     NITAKRAKLDILVENTGRVNFTKVLRGERKGITKQVTLAGKPVTGWDIYSLPMTNTQN
                     LHYSSAPCAGACFYRATFQVEKPADTFLDTSAFTKGEVWLNGHALGRVWNIGPQKTLY
                     VPGPWLKAGENEVIVFDLDGAPGRSLQGLKKPVLDAQVKK"
     gene            7140..8351
                     /locus_tag="VFA02_02350"
     CDS             7140..8351
                     /locus_tag="VFA02_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HZU08717.1"
                     /translation="MTTRRDFLSQAAVTAAGWVAIPSGAQTADSVKRLAPPKKPVIVT
                     RDTGDNTIDEAWKMLLDGADTLDACNHICMGREDDPNDHSVGYGGLPNEDGVVELDAC
                     CMHGPTRMAGSVGAIQNIRHASLVARLIMQRSGHVMLTGQGAEHFAVMHGFKREDLLT
                     DDARKMWLLWKESRSSQNFWGPPMSAPGGKDANPAPVLNHSAIPRHPGPEWSEIIRVR
                     SQQLSAMAGDLGIPEHKRAVAVQQVLWPTTGTVHVSVVNTKGEMSGATSTSGQAFKIA
                     GRLGDSPIIGAGCYTDQDVGSAGATGSGEENIKVAGAHTIVELMRQGMSPKDAGLEAL
                     RRIVRNYNGDMKKLRYLDMIYYIVRKDGAYAGCSLWSTTASGSPKTFMVHDGEKRVEK
                     CASLLEGTTTN"
     gene            complement(8641..9111)
                     /locus_tag="VFA02_02355"
     CDS             complement(8641..9111)
                     /locus_tag="VFA02_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU08718.1"
                     /translation="MEAMREGLMPHITVSDAAAAIDFYKKAFDAIEVACHLAPDGKRI
                     MHARLDLFDSVLMLNDDFPEYCGGKSNTPQALTGSPVTLHLQVNDARKVWDSAVAAGA
                     NEMMPLQEQFWGDLYGVFTDPFGHRWSIGQRVKQLSDSEIQDGAKEAFQHAQPA"
BASE COUNT         2064 a         2783 c         2422 g         2009 t
ORIGIN      
        1 ccgagtcccg ggatcacgaa gaggccgttc ttcttggtct ctttgatcgc cgtttcggca
       61 agcaggtcca gaaatgcagc tgcctgcttg ttggtgagtt ccagcttttc cgccaggtgg
      121 cggacaaggg ctgtcttggt cattccagtt gccattttat gtagatcctc ctggttctta
      181 tttcgctttc ccaaggcctg gaaccctgtt ccggctggtt ccgcgttgct tcggttttct
      241 tgttccgcga ctcaaacagc gctgaagccc tgtattcatg cgggcattca aaaaaacctt
      301 ccgccgttta ccttgcggct ttttgagggc tttgtcaacg ggaaaagcgg catttccaca
      361 ggtttttttg ggttttttgg catttttgtc ttatttttgg ctatttttgt ggattttcag
      421 cggattcggc gcaatctgcg tccaccgaag gcctcgccgg cagcggctgc aagcgggctt
      481 ttcactggag gaatgatgga tagtgtacgg cattttcaca gattctatac actccatttg
      541 cactgtgcag tgccgccagg tcgctgttgg ccgcggcgac tgtagtttcc gggcatggtc
      601 cagactatct gtgaaaacgc tatagtcccg tttacattag tttgaaaata tattgccgct
      661 tcttttccac ttcatggcgg aagggccggt tcaggaaatc cacaatagga gcggccagcc
      721 ggaagcgcgt gacaatctcc ttcaccagcg tgctcttcag cgctgtttcc gcaggcagac
      781 gcgcaaacac gccccactgc tgctgcttga tgaggtctat tcccggatgg tcggcaggaa
      841 atcccttcgg cggacgcgaa agcgcgcgca catccagatc gaggtccatc attttgcgca
      901 gctttttatc cttcagcagt ttccggaact cttcgtgctg ctccagcaac tgcattctta
      961 tggcaaacaa ctgctcgcgc tgcggcatat agatgcctgc agccacgatg acttccgccg
     1021 gcgaaaagtg aaagtaatat ccaccgcccg aggtcttttc catacccaga cgcgcccacc
     1081 acgcagccac atgcgtctta tacggcgtct tatcagcaga aaagcgcgta tcgcgataga
     1141 tgcgcatcat gctcttttcc gccggacgca catgcgcagg cgcaaagtca gccattgcgt
     1201 ctgtgacttg ctcgatgacc gccagcatcg ggcgcttcag ctcgcgctca taaatttccc
     1261 gccgcgcctg gaaccactcg cgattgttgt tgcgcctcag tccgcgcagg aacttcagcg
     1321 cctgttcatt gaagtatgga ttggctggaa ccacgctgtt tttcggcatg gttccaatgt
     1381 acctaagata ccgcgctctc tgccttctcc tgctgatgtc cgttcgtagc ttcttcttcc
     1441 accaccagct cgtctaccag gatcttcatc tgtgcgccca gctcgtgaaa tttctctttc
     1501 cactctggtg aaaagtcctg gttccaggaa agtctcagcg tgtcgtacca gatgttctgc
     1561 gcctgccaaa gattcagctc gaaaggaagc tcgctcaccg tccgcgccgt cagcagcgcg
     1621 tcgtccagca ggtccatgcg ctcgggcgca gcgtgcaacg cgaccatcac acgcttcatt
     1681 ttcaggtcaa caatgtagcg cagcaggccg gagtcgagcg caatcttgtc cgtgtctgca
     1741 atgcgcagcc atgtccgtac ctgcaatgcg tcaatgggac tgctgtcaat cgcacggcgc
     1801 agccccgcat ttacggcaaa gtcggctgcc agttccagcg cgcgcggctt tggaaagccg
     1861 ttcctgctca ggaagtgcag caatgatgca tggccttcgt aaatcgcaat caggctcgac
     1921 tccacctcgg agataggtgg ctgaagaatc atgttcagaa tgcgccgctg ctcgtccttg
     1981 aacagtgact ggatggagta cgaaggtccg ctgaaagtgc ggtcaaagag ccgtacaact
     2041 tccggaaagt ctgcattcgt caccgcggcg cgcgccttct ctgcaaactc tctgtatttc
     2101 tcgctctgcg attcatcgta gcgctgcaca acggcgttaa tgttctggtc cccaaaatga
     2161 acaacagcaa aactcgcttc ctgactttct tctgtaattt gcgaaatgat gcgggcctgc
     2221 ccaagcagca gtcgcgagtg tcctgacgtt atgacttcat aggccagcct tctcgccgtg
     2281 tagcagtaga gcgttgcctc gtcaggataa ttgctgaaca gcgagctgat ggcataatgg
     2341 gccgccacct gctccaggcc gaccttcagc gtgctgattc tgcgcagata gacagatgcc
     2401 ccatcgcctt cctgcggcaa attgctcttt gccttacgca gaatttcaat aaagtgttct
     2461 tcaaggcccg ctccccgggg cccgaaaagc tgcgccgcaa gctgcaggac acgcgcggca
     2521 tacgcaatca cctgcgacgc ctcgatgcct gagatctcat cgaagaacca tccacagctt
     2581 gtgaacatca gcagcgcgtg gcgttgcagc tccatcagct tgagagcagt tatgcgctcc
     2641 tctgcattca gctcgcgctt tgcgtatctt gccaaaaatt gattcaggtc cgcgcggtcg
     2701 agcacaacat gaatgtagcc gtcgcgcgct gcccacacat ctttgaacag cggccttgcc
     2761 gtttcctccg tcagaggagc aacggcatca cgcagccaat ccagtgcctc gcgcaaaggc
     2821 gcgcgccact cctggttcca tccgggatgc ccggcattgc agccgcaatt cgaacgccag
     2881 cgctcaatgc catgcgagca gctccacgaa gtgttttcag caatctgcgc ctcccactgt
     2941 ggcggaaatt tttccaggaa ctcgccataa ttggtcaggg ctatgtctgg attttgttca
     3001 atcagcttca gtgcataaga aagcgccatc tcaccctgct tgtgatggtg tccatagctt
     3061 tcgccgtctg tcgcaatatg cacaagctgc ggagcatctt catctgtaaa accgcccatc
     3121 agtcgtgcgg caaatgtctc tccgctattc agcaggcctt caaacgcgac tgcgcgtgaa
     3181 cgcggcccat cgtaaaagaa cactgcgatt ttcttcttgt tcttcagccg gatgatgtat
     3241 ggatgcttcg tatcgagcgt ttcggcagag gtttccatcc agggcgcgtc tttgtccttg
     3301 cccatcggac gcacgcgggc gcactggtga ggggccagca ctgtgaattg gatgtgatgt
     3361 tccgccagca gctccagcgt ttctgaatcc accgcggtct cggccagcca catgccttcg
     3421 ggcctgcggc cgaaacgccg ctcaaagtcg gcaatgcccc agcgaatctg cgtggtcttg
     3481 tcccgccggc tcgccagcgg catgatcatg tggttgtaca cctgcgccat cgccgatccg
     3541 tgtccggaaa acttcatctg gctggcatgg tcggcctcca gaatcatccg gtaggttctt
     3601 ggtgcgcact gttccagcca ggaaagcagc gttgggccaa aattaaagct catatgcgca
     3661 tagttgttca gaatgcggat gatctcgcct ttctggttca caatgcgcga agcgccgttc
     3721 ggcgcataac actctgaggt gatgcgctca ttccagtcat gaaacggcgc agcggagtcc
     3781 tgcgtttcca cctgctccag ccacgggttc tcgcgcggag gctgataaaa atgtccatga
     3841 atacagatgt agcgggttgc ggtcggcatg aagtgggtcc ctcgttctct gttttactga
     3901 ttcagatgca gaaatgcacc ggttcccctg cttcataccc ttccccttcc aggagggctc
     3961 tagattgcag tgcttgaaag cggcaccttt cggcttgcat aatctgcttt ttgccactgc
     4021 tgcgtctgcg cgatggaaat gatgtgcaga ataaagaaga atggcacacc aaatgttggg
     4081 atcaggctca aaggcaatgc tgttatcggg tccatcgtca ccgggccgtg gctgatgaat
     4141 cctgtcgtta ctcctgaccc gatggccgta actaaatcca gcatccccag aatctgccat
     4201 agaatgaagg ccgcgcgatg cggacggtcg gccagtttca aggcgatcag aggtgcagtt
     4261 acaccaatga agagatcacc ccatccggcc ggcaatgcaa aactgccggg cagaatcttg
     4321 tacgtgtaga gcgcaaggaa cccgaacccg gcgatgcgcc agctttgcaa catcgtcaat
     4381 aggcgcaggt caagcgacaa cacaaaagca cggaagtcct gcgaagaccc gtaccacgca
     4441 aggaatatga gcacaggtac aagtaccgcc aatccaagcg caatgggagg cgtagatggc
     4501 ggattttgaa atatgccgag tcctgatgcc gtcagcgcta aaataaacca cgctgctatc
     4561 agccacgaaa tgggtctgcc gtacttgtcg gttttcataa taatctctcc tgatttctcg
     4621 ccagggtggc gatctcgtcg gtaagttgga aaatatcgtg ccagtgcttg tcgccaagct
     4681 gtctggccat cgtttgctgt gcttttttcc aggcaggaag cgtgcgctga agcttctctt
     4741 tgcctgaaga cgacaggccc agccgccact gcctgcggtc tgtgcctttt ttgcgagaga
     4801 tccacccttc ccggtaaaga atgtccagcg tccgcgtaag agtagtgctg tccatggcca
     4861 gaatttcgcc caaccgcccc tgagatacct cgccaacagc ggaaagcact tgcaaaaccg
     4921 tgagttgggt tgcccgcaat ccatgcgggc gcaaggcttc ttcatagcac tgggtcaaag
     4981 cgcgcgccgc gcgacgaaga ctggcgcaga ggcagggcag ggaaggcagg actggctctt
     5041 tcatatcaga aatggatgta tatgcatcca tattggactc acgctttgaa agaaataaat
     5101 ttaaactcct gaatcatgta gatataggtc gtgaagccct gccacgaaac tacagtcagc
     5161 gcgaccagca gggggtcggc gacactgcac agtacaagta cagaagctgt gaaaatgcct
     5221 taaaccctgt acactactct ttcgcagaca tcaggagaaa accgcaagca tgaaacgcat
     5281 tctcgccaca acactcctct ttgcaaccgt tttcatcggc caaacgtcct tcgctgcaca
     5341 accgcacacc ttcaaggctg aaaatggcca gtttacgttg gacgggaaac cattccaggt
     5401 catctccgga gaaatgcact atccacgcat cccgcgcgca tactggcgcg accgcttacg
     5461 catggcgaag gccatgggcc tgaacactat tacgacgtat gtcttctgga atgtgcatga
     5521 acccagaccg ggtgtctacg acttttcggg caataacgat gtggcggaat tcatccgcga
     5581 ggcccagcag gaagggctgt acgtcatact ccgccccggc ccctatgtat gcgccgaatg
     5641 ggacttcggc ggttaccctg catggctgct caaagaccac gacatggtgg tccgctcacg
     5701 tgatccgaag tttctcgcag ccgcgcagag atggatcaaa cgcctcggtc aggaacttgc
     5761 tccactgcaa atcggcaatg gcggccccat tattcttgtc caggttgaaa acgagtacgg
     5821 ctcttttgat aaagaccacg actacatgga agacatccac cacatgctcg tagatgccgg
     5881 attcaccaaa gcgcagctct acaccgccga tggccccgag caggtccccg acggctctct
     5941 gccagagctg cccgcagcta ttaattttgg ccccggcgaa tcacaaaagg gctttgctac
     6001 tctgaagaag ctgcgcccgg aaggcccatt catgaacagc gaatactggg atggatggtt
     6061 cgaccactgg ggatccaaac atgcgaagac ggacgcacag cagcagaact ccgaccttga
     6121 ctggacactg cgacagggct actccatcag cttgtatatg tttcacggag gcaccagctt
     6181 cggctggatg aacggcgcca actccaatgg caaaaactac gagccggatg tcaccagcta
     6241 cgactacgat tctccgctcg atgaaagcgg tcgccccacg ccgaagtact tcctcttccg
     6301 cgacacgatc gcgaaagcca cgggaaagac cccgccgcca gtcccgccgg tcgcgcctcc
     6361 aatggaggtc ccggccttca gcctcactca atcggcatcc ctctgggaca acctgcccag
     6421 gcccacacac agcgagcagc cgctctcgat ggaagacctc ggccaggcct ttggatacat
     6481 cctctatcgc acaaccttaa gcggcccggt tacgggtgac ctcgtcctcg accagctcca
     6541 cgattatgcg cagatttatg tagacggaaa gctcgtggga acgctcgacc gccgcctgca
     6601 ccagaactcg ctcccgctga acatcacagc aaaaagagcg aagctcgaca ttctcgtcga
     6661 aaatacaggc cgcgtgaact ttaccaaagt tcttcgcggc gagcgaaaag gcatcacaaa
     6721 gcaggtcacg ctcgcaggca agcccgttac cggatgggac atctattcct tgccgatgac
     6781 aaacacgcaa aacctgcact atagcagcgc tccctgcgcc ggagcatgtt tctatcgcgc
     6841 aacattccag gtggagaagc ccgccgacac cttcctcgac accagcgctt tcaccaaagg
     6901 agaagtctgg ctgaacggac acgccctggg ccgcgtctgg aatatcggcc cccagaaaac
     6961 actgtacgtt cctggcccct ggctgaaagc aggagaaaac gaggtcattg tcttcgatct
     7021 ggacggtgct ccgggacgca gcctgcaggg gctaaagaag ccggtgctcg atgctcaggt
     7081 gaagaaatag cgtttccgaa tgatatgctc cccttaaaac atctttcaga ggagcacgca
     7141 tgaccaccag gcgagacttt ctttctcagg cggccgtcac cgcagccgga tgggtggcca
     7201 taccctccgg tgcgcagacg gccgacagtg tcaagcgcct cgctccaccc aaaaagcccg
     7261 tcatcgtcac ccgcgatacg ggcgacaaca ccatagatga agcctggaaa atgctcctcg
     7321 acggtgccga cacactcgat gcctgcaacc acatctgcat gggccgcgaa gatgatccaa
     7381 acgaccacag cgttggatac ggcggcctgc ccaatgaaga cggagtcgtc gagcttgacg
     7441 cctgctgcat gcacggcccc acccgcatgg caggttccgt cggagccatc cagaatattc
     7501 gccacgccag cctcgtcgcc cgcctcatca tgcagcgctc cggacacgtc atgcttaccg
     7561 ggcagggcgc agagcacttt gccgtcatgc atggctttaa gcgcgaagac cttcttaccg
     7621 acgacgcccg caagatgtgg ctcctttgga aggaaagccg ctccagccag aacttctggg
     7681 gcccgcccat gtccgcgccc ggaggcaaag acgctaaccc cgcccccgtt ctcaaccaca
     7741 gcgccatccc acgccatccc ggccccgagt ggtctgaaat catccgcgtc cgttcgcaac
     7801 aactttcggc aatggccggc gacctcggca ttcctgagca caagcgtgct gtggccgtcc
     7861 agcaagtgct ctggcccaca acgggcactg tacacgtctc agtcgtaaac acaaaaggcg
     7921 aaatgtccgg cgcgacttcc acctccggac aggcattcaa aatcgccggc aggctgggtg
     7981 attcgcccat catcggtgca ggctgctaca ccgaccagga cgtcggttcc gcgggcgcaa
     8041 ccgggtctgg cgaagagaac atcaaggtcg caggcgcgca caccatcgtc gaactgatgc
     8101 gccagggcat gtcgcccaaa gatgccggac tcgaggccct gcgccgcatc gtccgcaact
     8161 acaacggcga tatgaaaaag ctgcgctacc tcgacatgat ctactacatc gtgcgcaaag
     8221 atggggccta cgccggctgc tccctctgga gcaccaccgc cagcggcagt cccaagacat
     8281 tcatggtcca cgacggagaa aagcgcgtcg aaaaatgcgc ttcgctactg gaaggcacta
     8341 cgactaactg atctcagatt tccggaacca gtaaaaaacc ggaaatcatt gccgtccaga
     8401 ctggagcggc cgaacatcgc gcagagcagg gaactgcatt tccgctaccc ctccattcaa
     8461 gccggagtgg aagttgagtg gggtatagcg catgtatatg tagagttcgt cattctacga
     8521 ccgaaggggg aagaatctta gcgatgtgag gctctggcca atcctgctaa gatactccgc
     8581 gcctccggca tgaatcatcc ttttcctgca tcacagtgaa acgaaaaata gtctgctgtt
     8641 ttacgcgggc tgggcgtgct ggaaggcctc tttggctcca tcctgaattt cagaatcgct
     8701 tagctgtttc actctctgcc caatagacca cctgtggccg aacgggtccg tgaacacgcc
     8761 ataaagatca ccccagaact gctcctgcaa cggcatcatc tcgtttgctc ctgcggcaac
     8821 ggccgagtcc cataccttgc gtgcgtcatt gacctgcagg tgaagcgtta caggcgatcc
     8881 agtcagggcc tgcggagtgt tgctctttcc accgcagtat tcagggaaat cgtcattcag
     8941 catcagtacg ctgtcgaaca aatccaggcg ggcatgcatg atccgcttgc catcgggagc
     9001 aagatggcaa gcaacctcaa tggcatcgaa cgcctttttg taaaaatcaa tggccgctgc
     9061 cgcatcgctc accgtgatat gtggcatcaa tccttcgcgc atggcttcca taggtgtttc
     9121 ctcctgagga agcgagtata cgcgcggaat actgcgcccc gcgacaaacc tggcacattt
     9181 tcaacagaat ttctcaaagg agagagagta agttcttatc ttatgtcccc ttctctcttg
     9241 gaagaaccgg gtaccgtgtg ggtgttccac tcaagcct
//
LOCUS       DASRBV010000055         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_255872, whole genome shotgun sequence.
ACCESSION   DASRBV010000055 DASRBV010000000
VERSION     DASRBV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255872"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /gene="bshC"
                     /locus_tag="VFA02_02360"
     CDS             <1..1095
                     /gene="bshC"
                     /locus_tag="VFA02_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HZU08719.1"
                     /translation="LGKGIRPVLDDAAEILGGSEVFEILEGCYTPDATFAQAFGCLLS
                     RIFADHGLILIDASAREFHQLGAPVFEKAITDADELRAALLARDKELAEAGYHSQVLV
                     SQQSSLLFLIDEETKARQPLRRKNGEWVAGKKIYSANDLLEILQVQPELLSPNALLRP
                     VFQDLILPTSLYIGGPAEIAYFAQSQVVYEKVLGRTTPILPRLSATLVEPAIAEVMAK
                     HGVAVNDVLSTHPDELAQRIGARSMPVEGKQKLAAAGNALDAELKSLTEWMHALDPGL
                     GRPADVAASKMRYQMNRLRRLAANFQLQKEQSLRRHVDAMYLALYPERHLQERVVGAI
                     YFLSRYGNGLVDTLVEHAAQECPGHKAIYL"
     gene            1142..1453
                     /locus_tag="VFA02_02365"
     CDS             1142..1453
                     /locus_tag="VFA02_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08720.1"
                     /translation="MPSKPQSAFEVSCPDCGAILKIDPETGAVIGHTSAPRKKTFEDF
                     ETAARAMREQDERRESLFRQAVDAEKNKNDVLEKKFAEALRKAKETPDTGKPLRDFDL
                     D"
     gene            1492..1812
                     /locus_tag="VFA02_02370"
     CDS             1492..1812
                     /locus_tag="VFA02_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08721.1"
                     /translation="MGLTKISHEIQERIPEPLAEFSWQRTVAAGSLVAGACLFLAGRR
                     KTALAVAAAGATVGLLEHPESAKELWDSLPQYLRKGQDFLVRVEDFVNEVAKQGSKLR
                     SAIG"
     gene            2104..>2643
                     /locus_tag="VFA02_02375"
     CDS             2104..>2643
                     /locus_tag="VFA02_02375"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZU08722.1"
                     /translation="MGAATVATDLRILTEGPHVHRNLSTPQLVESAIARGEASLAANG
                     ALVALTGARTGRSPKDKFTVEDDTTRDRVHWGKVNQRFDSDKFEALLERVVRHMAERD
                     MYVMDLYAGADPAYRLPITVIAEYAWHALFVKQLFVRPQPSELENHMAEFTVIAAPEF
                     EAVPERDGTKSSTFILADFT"
BASE COUNT          564 a          703 c          805 g          571 t
ORIGIN      
        1 ctgggcaaag gcattcgtcc ggtgctggac gatgcggctg agatactcgg gggcagcgag
       61 gtctttgaaa ttctggaagg ctgttacaca ccggacgcca cctttgcgca ggcctttggc
      121 tgtttgttgt cccgcatctt tgccgaccat gggttgattc tgatagacgc ttcagcacgg
      181 gagtttcatc agcttggcgc tcccgtcttc gagaaggcca ttacagatgc cgatgagcta
      241 cgcgctgcgc tgcttgcccg ggacaaagag ctggccgagg ccggatacca ttcgcaggtg
      301 ctggtcagcc agcaaagcag tctcctgttt ttgattgatg aagagacaaa ggcccgtcag
      361 ccgttacggc gcaaaaacgg ggaatgggtt gccgggaaaa agatttactc agcgaacgat
      421 ttgctggaaa ttcttcaagt acaacctgaa cttttaagcc cgaatgcgct gcttcgtccg
      481 gtctttcagg acctcattct gccgacgtcg ctctacattg gcggccctgc cgagattgcg
      541 tatttcgcgc agtctcaggt ggtctacgag aaagttctgg gccggaccac gccgattctg
      601 ccgcgcctga gcgccacgct ggttgagcct gccattgcgg aagtgatggc aaagcatgga
      661 gtggctgtga atgacgtttt gtctacgcat cctgatgagc tggcacagcg catcggcgcg
      721 cgttctatgc cggtggaagg caagcagaag ctggcagcag caggaaacgc cctcgacgcc
      781 gagttgaaat cgctgacgga gtggatgcat gcgctggatc ccggactggg gcggccggca
      841 gatgtggccg cttccaagat gcggtaccag atgaaccggc tgaggcggct tgcggcgaac
      901 tttcaactgc agaaagagca gagtctgcgc cgccatgtgg acgccatgta tctggcgctc
      961 tatccggagc ggcatttgca ggagcgtgtc gtgggggcga tctatttcct ctcgcgctac
     1021 ggcaatggtc tggtagacac tctggtggaa catgcggcgc aggagtgtcc cgggcacaag
     1081 gcaatttatc tctgagcgct gctggcgcaa gccccttcgg ctggcctaca atttcctcgt
     1141 aatgccgtcg aaaccacaat ccgcatttga ggtatcgtgc ccggactgcg gcgcgatcct
     1201 gaagattgat cctgaaacgg gcgcggtgat tgggcataca tctgcgccgc gtaagaagac
     1261 ctttgaagac tttgagaccg cggccagggc catgcgcgag caggacgagc gccgggagag
     1321 ccttttccgt caggctgtgg atgcggagaa gaacaagaac gacgttctgg aaaagaaatt
     1381 tgccgaggcc ctgcgcaagg cgaaagagac gccggacacc ggcaagccct tgcgggactt
     1441 cgatctggat tgagaaaaga tcttaaccca tatttttatt caggagatgc aatgggtctg
     1501 acaaagattt ctcacgaaat tcaggaaagg attcccgagc cattggctga attttcatgg
     1561 cagagaacag tcgccgccgg atcactggtg gctggagcat gtttgtttct ggctggacgc
     1621 cgtaagaccg cgctggcggt ggccgcggcc ggggccacgg taggtttgct ggagcatcct
     1681 gaatcagcaa aagagctgtg ggacagcctg ccgcagtacc tgcgcaaggg tcaggacttt
     1741 ctggtccgcg tggaagattt tgtgaatgaa gtggccaaac agggaagcaa gctgcgcagc
     1801 gcaattggct agtgtatttt catttcgctg tgatgcacgg aagggatgat ggtcattccg
     1861 gggtgtgggg aaaatctcag caggattgct gaagcctcgc atcgctaaga ttcttcctcc
     1921 cttcggccgg cggaatgacg gactctccat atacgaaaat gctctggccc attgctatga
     1981 accgccccgt ttgtagccgt acaggatgaa cgtgggggtg gatgtctttt ccgcctttcc
     2041 gggcgccttt gccatatcac tgcatcaggt ggcacaataa acggtttgcc tgaaggagag
     2101 ttcatgggag cagccaccgt ggccaccgat cttcggattc ttaccgaagg cccgcatgtt
     2161 caccgcaatc tttctacgcc acaactggtt gaatcagcca ttgcgcgcgg agaagccagc
     2221 ctggcagcca acggcgcgct ggttgcgctt accggagcgc gcacgggccg ttctcctaaa
     2281 gacaaattca ccgtagagga cgataccacc cgcgaccgag tgcactgggg caaggtgaac
     2341 cagcgcttcg attccgacaa attcgaggcg ctgctggagc gcgtcgtccg ccacatggcc
     2401 gaacgcgaca tgtatgtgat ggacctttac gcgggtgctg atccggcata ccgccttccc
     2461 attaccgtga ttgccgaata tgcatggcat gcgctctttg taaaacagct ttttgtaaga
     2521 ccgcagcctt ccgaacttga aaaccatatg gccgagttca cggtcatcgc tgctccggag
     2581 tttgaagcgg tgcccgaacg ggacgggaca aagtcgagca ccttcattct ggctgacttc
     2641 acg
//
LOCUS       DASRBV010000056        12794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_663013, whole genome shotgun sequence.
ACCESSION   DASRBV010000056 DASRBV010000000
VERSION     DASRBV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663013"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFA02_02380"
     CDS             <1..832
                     /locus_tag="VFA02_02380"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HZU08723.1"
                     /translation="YWPHENDVIHDPAYQELYARWFEFGTFCPVFRTHGHRAENEMWA
                     YDKVEPILIDFDKLRYRLMPYIYSLAWKVTSEDYTIQRPLVMDFRADPNTWNIGDQFM
                     FGPALMVSPVLKSNATRRSVYLPPAPFWYDFWTGAKVKGGQEMEAEAPLDRIPLYVRA
                     GSILPLGPEIEYAEQDPGGPIELRIYRGADGSFDLYEDSGDSYDYEKGQHAIIPIRWE
                     EKGGTLIIGAREGSFPGMTQSRKFRIVFVKEGHGTGEAVSASSDREITYNGKEIRIQQ
                     "
     gene            complement(1050..2597)
                     /locus_tag="VFA02_02385"
     CDS             complement(1050..2597)
                     /locus_tag="VFA02_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HZU08724.1"
                     /translation="MATSNQVAPEKSSSHALEEERREFEAVAAALTNSSRLLRLITYL
                     GEKYFGGDADQLQEYNIATEVFGRSRTTFNPGEDAIVRVEFHRLRKRLREYYEAEGKD
                     HPIQLTVPPGTYSPVFVRRQPLAETQPPNHEVPEAPVFAPLAPSLPPGRRRWPYLLTI
                     GVLVFAAVALYLTFRSQRNGRERASAAVPSPAATPPSASTAFASVPLRILAGYSGKPQ
                     MDSAGQIWEPDQYFQSGGNWHVSADYVANSTNPMLFEHWRNGQFSYNVPLRPGTYELH
                     LYFMALDRGLNSPPTFSVSMNGDLLLPDFDIYSDTMGTYVADERVFRDVHPEPDGFLH
                     LDFTGGRSTATLNAIEILPGIPHHQVPVRLVMQSRPVTDHSGRLWHPDDDYQGGYASV
                     KNVTVSGTADPDLFTAERYGHFSYSIPVDTRDRYTLVLYFAELYFGPNASGTGGVGSR
                     VFRVLCNGNTLLDNFDIYKEAGSLHAITKTFYHLKPTAQGKLNITFEPIVNYATVSAI
                     EVLDESQ"
     gene            complement(2897..4852)
                     /locus_tag="VFA02_02390"
     CDS             complement(2897..4852)
                     /locus_tag="VFA02_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HZU08725.1"
                     /translation="MSQQIRRVHSVYTCVLGALLLGCTPFAALLLGCTSFALPIKAQA
                     QATASVSINTSSAMGNVPAQGYGVDSSVYDNYMTSPDLGASLKTGGFNAIRYPGGSYA
                     DIFNFLSGSDNTLNNGGYFASGDTFDNWIADLIQPSGAKGVITVNYGSNPNNNGPASP
                     SEAASWVQYANITNNYGILYWEIGNEIYGNGYYSSGNWEYDLHVLNQSASARLGNPAL
                     SPTAYGTNAAEFIKAMKAVDPNIKCGVFVSTSPWVPGWNQDVLTAISNSLQGTGYTLD
                     FVILHWYPSGTNAQVLAAPGQQISSQVAQVRSDIQKYYTLSNGNQLQLLVTETAATSN
                     GGIFPYLFTADEFLTWFEQGASNVEYQELHSGVYEASSANTPIGPWYGVQFDSILARP
                     GDRLVSASSSNSLLRVHGVRRSDGQVGVVLINDDPSNDTTVSVNISGATLASSGIKYT
                     FGNANFPSGSDTPSSGIASNSISGVGNNFTITVPAYSTVAVVIPQTSNSSGNLIANGN
                     YVISNYQTGLALDDYQFSKTAGTFMDMWPVNGGSNQVWTVTNLGNNYIYLTNAYSGLA
                     LDVYQASTSPGAKIDQWYWSNQSNQIWKVVSMGNGTYELLSQHSGLALGIPSATTGSG
                     SSSLLNGAGLAQETVTGAADQLWIFNN"
     gene            5712..9449
                     /locus_tag="VFA02_02395"
     CDS             5712..9449
                     /locus_tag="VFA02_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU08726.1"
                     /translation="MYRPSFRAIWMAMIAISFSISLSAQTAGSIAGKVTDITGAVVPG
                     ANVMVQNEGSKSQWSITSNNEGLFRFAAIPPATYTMRISKPGFETWTVTGIVVHPGDS
                     LTVPAIALKVGRTDVSVTVTATGAGVSLDSPEHSNLITSAEINRLSTQGRDVAELLSI
                     TPGFTINAGTNLQNEGPGGLYGYQTMGFGSGQVGSWGANGAAPQQGLVNVTADGNNLI
                     DPGDMGGQVSNVNMDQVQEVKIQTSNFGADQSKGPIVVNAVGKSGSADFHGGLYTYFR
                     NSALNSNDWLSKYYGAARPTLRYFYPGGTLGGPVIIPHTHFNEKKNLTFWTGFEYYGQ
                     DASQGLATSFIPSPAMMSGDLSTETIAKELNVSATDLAANCTADYSQTPTYNNIGGLC
                     WSPNGSTDQMGATVNNGQVKNIDPGISTISRLWPVANRTPQPVITAGQTQYATDGINY
                     VKNVMQTHNGFQFHTRVDESFTDSLKFYVVYNWERINDESAMNNIYYNPGGTVPYPTP
                     LYSYGHAHYLTVDLTKTLGSSLTNELIASGVYMSQPEQFGNRARAQTTGTAWEAAGYS
                     QGHLNVGQTQLPRIISYDGGIPAFAFGYVPPTSQFLRKFSWNIADNLTRVYGKHTFKA
                     GVYAEQTGNSNATLGSQLNGTLSFMRWDGCYINQTSKTANPPGTANLGNVIGNFLIGC
                     PLGYAQDNSDPVINLRFRTFEGYITDEWKATSNLTLTLGIRLSHLGPWTDAHGIGIAV
                     WDPSSISQHTLYPDTADSTTWPGITWHKRNPRIPNAGVPTRALFYAPRVGLAYDLHGN
                     GRTVFRGGWGMYYSHDAAGFSGGGANTSTGLATYTTPGNISCTFGQLFTTQYVPCGAY
                     SSTPTTITPFSVSAVNPKDDRMPLTYNYNFTVDQQGPWSTLFEIAYVGNQSTDLSSLG
                     NLQNQNVIPLGAFFKPDPLTGQLNPPSSIPNSADYRPYPNYQFINVPSHIGWANYNSL
                     QASWNKQKGALIWGANYTWSKAMGVRGNWDTGSIADPVDPEHDYGIVSFDRPQALNFT
                     YSYDVGQKYNGNKILKQVINGWQISGINSIQSGPDLAVLNGNTNYNINGGVDYTSGGK
                     TVSVPIGAAEWLGSSDYLLQPAVTCDPRTNLKKDQFVNGGCFTLTPQGTQGWWNLPDN
                     HGPAYFRWDLSVFKTFNITERQNMQFRVSGFNFLNHPLTSFNNNNLSTLNLNVGNCAG
                     CNATTPEQAIQNATITNASTFGSTAFRTGVRIVELGFKYNF"
     gene            9625..11205
                     /locus_tag="VFA02_02400"
     CDS             9625..11205
                     /locus_tag="VFA02_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HZU08727.1"
                     /translation="MFRPVLIALGFSVFSVLPIAAQSVQTSPRQIVADLKDTTGPVDH
                     FFDLSVGSDYPGTLVRNDSQAQLKLAVDELGFRYIRFHAIFHDVLGTVQIENGRTVYD
                     WSGIDRLYDDLLARHIRPFVELGFTPQALATSQNSIFYWHGNTSHPKPQGWQDLVSAF
                     IRHIEQRYGKDEVRTWFFEVWNEPNLSGFWENADQQAYFQLYDLTARTIKSVDPALRV
                     GGPATAGAAWVPEFLAHVKQSGAAVDFVSTHTYGVDGGFLDENGKSDTKLSPSPDAII
                     GDVRRVREQISASPFPGLPLYFTEWSTSYTPRDSVHDSYISAPYILTKLKACQGLAQG
                     MSYWTYTDLFEEPGPPTAPFQGGFGLLNPEGIRKPAYFAYKYLHALQGSSIATNDAQA
                     MLATRDGDLTAVLWDFEQPTQKVSNRSFFTKPIPSHPSVPVQLKVKGLVPDAAYHLEI
                     HRTGYHANDAYTAYLEMGSPKELSAAQVAYLDRLTQDLPETDRTVHSSPDGSLDFVLP
                     MNSNDIVLVKLERARGAK"
     gene            11301..12044
                     /locus_tag="VFA02_02405"
     CDS             11301..12044
                     /locus_tag="VFA02_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU08728.1"
                     /translation="MPEHNKKVALITGANRGIGFETARQLGQKQVTIVVGARSPESAK
                     ETADKLTAQGIEAYPVVLDVTKEADRKAAASFIAKKFGRLDILINNAGIGGDGGLLNA
                     HTISTSQDELQRVFNTNLFSVVAVTREFLPLLKKSPAGRIVNLSSILGSLTLQAMPNS
                     PIAPMKAFAYNASKTALNQFTVHLAAELKDTNIKVNSVHPGWVKTELGTQHAQLEVAD
                     GAKTSVEFALLGADGPSGKFVHDGQELPW"
     gene            12207..>12794
                     /locus_tag="VFA02_02410"
     CDS             12207..>12794
                     /locus_tag="VFA02_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU08729.1"
                     /translation="MSKGTETRKRIITKAAPLFNRKGFEGCSMQDIVEAVGLEKGSLY
                     GHFATKEALALAAFEYSWGETCTARLAGMDTATNAIAKLRIHVDNFLSSPSIAGGCPL
                     LNTITDSDDGNPVLKRAAREALKQWRLLLVTIIEEGQRRGEIRSGVVPEEIAALLLSL
                     LEGAMALDRLDKKAGFLQNAVRHLKSYLDSISSEQR"
BASE COUNT         3012 a         3414 c         3477 g         2891 t
ORIGIN      
        1 ctactggcca catgaaaatg atgtgattca cgaccctgcc tatcaggagc tttatgcccg
       61 atggtttgaa ttcggaacct tctgccctgt cttccgcacg cacgggcacc gcgcggaaaa
      121 cgagatgtgg gcgtatgaca aggttgagcc aattctcatt gacttcgaca agctgcgtta
      181 tcggctgatg ccgtacatct attcgctggc gtggaaggtg accagcgagg actacaccat
      241 ccagcgtccg ctggtgatgg acttccgcgc cgacccgaat acgtggaaca tcggcgacca
      301 gttcatgttt ggcccggcgc tcatggtcag tccagttctg aagtcgaatg ccacgcgacg
      361 cagcgtgtat ctgcctcccg cgccgttctg gtatgacttc tggacgggcg cgaaagtaaa
      421 aggcgggcag gaaatggaag ccgaagcgcc gctcgacaga attccgctgt atgttcgcgc
      481 cggatcgatt cttccgctgg ggccggagat tgaatatgcg gaacaggacc cgggcggccc
      541 aattgagctg cgcatctatc gcggggccga tggcagcttc gacttgtacg aggattccgg
      601 tgattcttac gactatgaaa aaggccagca cgccattatc cccattcgat gggaggagaa
      661 gggcggcact ctgattatcg gcgcgcggga aggatcattt ccaggcatga cacaaagccg
      721 taagttcaga atcgtattcg tgaaggaagg tcatggcacg ggtgaggccg tttctgccag
      781 ttccgatcgc gaaatcacat ataacggcaa agaaatacgt attcaacaat gatgtctgcg
      841 tcgaggactc tgattctccg gaaataactg ggcactgttt gcatacaatt ctgcccacaa
      901 ccgtttgcca gcatgaaaca gtgctggcgg acagcagaat gttatatccg gaaagccgtc
      961 cgctccatac ttgcgcatgt gcgtacccgg gtcctcgagc aaggcaaaat ccagcgtata
     1021 tttgactcgc ccgcttgtga tgcggggcct tattgagact cgtcgaggac ttcaatggca
     1081 gaaactgtgg cgtagttcac gatcggctca aatgtaatgt tgagcttgcc ctgggccgtg
     1141 ggcttcagat gatagaaggt ctttgtaatg gcgtggaggc tgcccgcttc cttataaatg
     1201 tcaaaattgt ccagaagcgt attgccgttg cacagtacgc ggaaaacgcg gctgcccacg
     1261 cctccagttc cagatgcatt cggcccgaaa tataactctg caaaatagag gaccaatgta
     1321 tagcggtcgc gtgtatctac aggaatggaa taagaaaagt ggccgtagcg ctctgcggtg
     1381 aagaggtccg ggtcggcggt gccggaaact gtcacgttct ttactgaggc atatccgccc
     1441 tggtaatcat cgtccggatg ccagagcctt ccactgtggt cagtcaccgg tctggattgc
     1501 atcaccagcc ggacggggac ctggtggtga ggaattccag gcagaatttc tatcgcattc
     1561 aatgttgctg tagagcggcc gcctgtgaaa tcaagatgca gaaatccatc cggttcggga
     1621 tggacatcgc ggaagacacg ctcatcggcg acataggtgc ccattgtatc ggagtagatg
     1681 tcaaaatccg gtagaagcag atctccattc atggagacgg aaaacgtggg aggactattc
     1741 aggccacggt ccagggccat aaagtacaga tgcaactcgt atgtacccgg gcgcagaggg
     1801 acattgtagg aaaactgccc gttacgccag tgttcgaaca gcatggggtt cgtgctgttt
     1861 gcgacgtagt cggccgacac atgccaattg cctccggact ggaaatattg gtccggttcc
     1921 catatctgac cagcgctgtc catctgtggc tttccagagt agccggccag aatacgcaag
     1981 ggtacggacg caaaagcggt actggctgat ggcggtgttg ccgcagggct gggaactgca
     2041 gccgacgcgc gttctcttcc attcctctgt gaccggaagg tcaggtaaag cgccacagcg
     2101 gcaaagacca gtaccccgat tgttagcaga taaggccatc tccggcgtcc aggcggcaag
     2161 gatggagcaa gaggtgcaaa aaccggggct tccgggacct catggtttgg cggctgtgtt
     2221 tctgcgaggg gctgacgccg tacaaatacc gggctgtatg tgccgggtgg taccgtcaac
     2281 tggatgggat ggtcttttcc ttccgcttca tagtattctc gcagcctctt ccgcagacgg
     2341 tgaaattcca cgcgcacaat cgcgtcttcg cccggattaa atgtcgttcg ggaccgccca
     2401 aagacctcag tcgcgatgtt gtattcctga agctgatcgg catcgccgcc aaaatacttt
     2461 tccccaagat aggttatcag ccgcaacagt cgcgacgaat ttgtcagagc tgccgcaaca
     2521 gcttcaaatt cccggcgctc ttcttccagt gcatgtgatg aggatttttc gggtgcgacc
     2581 tggttgctcg tggccaaaat ttcttccccg gtagaacttg atgatacctg caggatgtac
     2641 gtgtttgcaa cgtaaccggg tggacagttg ggccccgagc tataaagatt gttttcaatg
     2701 agatacagct atacatccca gaaacattcc ggctggagat tgtacgcgtt attttataca
     2761 tcccgatgtt gcggaaatga catcaagtca cgatgactct gtgaatccgg tgtcaatccg
     2821 gtcgggcatg ggtctgaaga ccattgcccg gatgcggatt gacgccggtg ctgtggagtt
     2881 gcgggaaaat ggcaactcag ttgttaaaga tccaaagctg gtcagcggcg ccggtcaccg
     2941 tttcctgggc cagccccgcc ccgttcagca gagagctgga accgctcccg gtggtcgccg
     3001 aagggatgcc cagggccagg ccgctatgtt ggcttagcag ctcgtaagtg ccgttaccca
     3061 tgcttacgac cttccaaatc tggttgcttt ggttggacca ataccattga tctatctttg
     3121 ccccagggct tgtagaggct tgataaacat ccagcgccag cccactgtag gcattcgtta
     3181 aatagatata gttgttcccc agattggtta ccgtccagac ctggttactg ccgccgttga
     3241 ccggccacat atccatgaag gtcccggcag ttttgctgaa ttggtaatcg tcaagggcca
     3301 gtccagtctg atagttcgaa atgacatagt tgccattggc aatcagattg ccactgctat
     3361 ttgaagtctg agggatgacc actgcaaccg tggaataggc cggtaccgta atggtgaagt
     3421 tattgcccac tccgctgatg gaattggagg cgataccaga gcttggcgta tctgatccag
     3481 aagggaaatt ggcgttgccg aaggtgtatt ttataccgct tgaggccagt gtcgctccgg
     3541 agatgttgac ggaaacagtg gtgtcgttgc ttggatcatc gttgatcagg actacaccga
     3601 cctggccgtc ggaacgacgt actccatgca cgcgcaacag tgaattggaa gagctggcag
     3661 agaccagccg gtctcctggg cgcgctaaga ttgagtcaaa ctgcacgcca taccaggggc
     3721 cgattggcgt attggcggat gaagcttcat agaccccgct atgcaattcc tgatattcga
     3781 cattggatgc cccctgctcg aaccatgtaa gaaactcgtc agcagtgaac aggtagggaa
     3841 aaattccgcc attggatgtt gcggccgttt cggtaaccag aagctggagc tggtttccat
     3901 tgctcagagt gtaatacttt tggatgtcgg aacggacctg cgcaacctgg gagctgatct
     3961 gctgccccgg agcggccagc acctgcgcat tggtcccgct tggataccaa tggagaataa
     4021 cgaaatccag cgtatatccg gtcccctgga gcgagttcga aatggcagtc aggacatcct
     4081 ggttccagcc cgggacccag ggtgacgtgc tgacgaatac accgcacttg atgttcggat
     4141 ccaccgcctt catggctttg ataaattccg cggcgttggt tccataagcc gtgggggaaa
     4201 gggcggggtt ccccaagcgg gctgaggctg actgattcag gacgtgcagg tcgtattccc
     4261 agtttccgct ggagtagtaa ccgttgccgt agatctcgtt accaatctcc cagtacaaaa
     4321 tgccgtagtt attggtgatg ttggcatact gcacccagga cgcggcttcg ctgggcgagg
     4381 ccggaccatt gttgttcggg ttagaaccgt aattgaccgt aatcacgcct ttggccccgg
     4441 aaggctgaat cagatcggca atccagttgt cgaacgtatc gccggaggcg aagtatccgc
     4501 cgttattcag cgtgttatca ctaccactga gaaagttgaa aatgtccgca tacgaaccac
     4561 cggggtagcg aatggcatta aacccgcccg ttttcagcga tgccccaaga tcagggctcg
     4621 tcatatagtt gtcgtagact gacgagtcca cgccataacc ctgggccggt acgttgccca
     4681 ttgctgaaga ggtgttgatg ctgacgctgg ccgtggcctg tgcctgtgcc tttatgggca
     4741 aagcgaaaga cgtgcagccg agcaggagcg cagcgaaagg tgtgcagcca agcaagagcg
     4801 caccgagaac acacgtatag acgctatgca cgcgcctgat ttgctgagac aaatactcag
     4861 agttccttat catcgagaaa acgattcctt tcaagttggg atgacccgta gatctggtgt
     4921 tttctgtcgt cgtttactcg cagttttcgg cgggggagga cgacaatgcc ggaaaaggta
     4981 gatttcattt ttatagtttg ggaaggttaa aaatgtataa agttgtacca gtatgcggag
     5041 gacattgtac ttatcttaaa atcaatggct tagacaaaag ccgttaacag cactggaaca
     5101 caatgaacat taacatcggg actgcgaagc cccgaaaaac ggggccatgg cctgctattt
     5161 cttttccgcc atccgctggc ttgacgaagt gtgcttctac ctagactgat tcatgagagt
     5221 gcgccttaaa accgagggtc cctggagcaa gtacctcaaa tgcggcaggg gctctggacc
     5281 gcatactttt taatgcaatg gggttgggat gagtttttca tccccggatg gcccttttta
     5341 tctgaaatgt tacgtagcgc ctcccgcgct gttttatcag gtacatatat cgcatgtacg
     5401 tcttgtgttt ccaacgacat ggaatgttac agagccgttt tggcgtcgtt gtacaagatt
     5461 gtacgagata gaactttact actgttatca gcgctatgca ggattcgttc cggtttctct
     5521 tttaagggaa agaggtcgcc caaggcaaga tgctgcccat tggatctaag cgctgactga
     5581 aacgaattcc aaaagacctg ttgaagcgca ccggtttaac ggcagaagta gtacttcctg
     5641 cggccaatcc agacaaggag gaccgatttt cctctacgca actcaacgcg atgaggacct
     5701 tggaggccag aatgtacaga ccgtctttcc gcgcgatatg gatggcaatg attgccatca
     5761 gcttttccat ttcgctctcc gctcagacag caggaagcat cgctggcaag gtcaccgata
     5821 ttaccggggc ggtagtgccg ggtgccaatg tgatggtcca gaacgaaggc agcaagtccc
     5881 agtggagcat taccagtaat aacgaaggcc tattccgttt tgcggccatc cctcctgcca
     5941 cctataccat gcgtatcagt aagcctggct tcgagacatg gacagtaacg ggaatcgtag
     6001 tgcatcccgg cgacagtctg acggtccctg ccattgcact caaggtgggc cggacagatg
     6061 tttccgttac tgtgaccgca accggggccg gggtctccct ggactctccc gaacacagca
     6121 acctgatcac gtcggcagaa atcaaccggc tctcaaccca gggccgtgat gtggccgagc
     6181 tactcagcat cacacccggc ttcacaatca atgcgggcac gaatttgcag aatgaagggc
     6241 caggcgggct gtacggatat cagacgatgg gcttcggctc gggacaggta ggctcttggg
     6301 gcgccaacgg ggccgctccg cagcaagggc tggtcaacgt aaccgctgat ggaaacaatc
     6361 tgattgatcc tggcgatatg ggtgggcagg tctcgaacgt caatatggac caggtgcagg
     6421 aggtgaagat acaaacctcc aatttcgggg ccgatcagtc caagggaccg atcgtggtga
     6481 atgctgtggg caagtctggc agcgccgatt tccatggagg gctttacacc tacttccgaa
     6541 acagcgcgct gaactcgaat gactggctct caaaatatta cggggcagcg cggccaacgc
     6601 tcagatactt ttacccggga ggtaccctgg gggggccagt catcattccc cacacccact
     6661 tcaatgagaa gaaaaacctg accttctgga cgggatttga atattacggc caggatgctt
     6721 cccagggact ggcgacctcc ttcattccct cgccggcgat gatgagtggc gacctgagca
     6781 cagaaaccat cgcaaaagag ttgaacgttt ctgcgacgga cctcgccgcg aattgcaccg
     6841 ccgattatag ccagacaccc acctataaca atattggtgg cctttgctgg tctcccaacg
     6901 ggtctacgga ccagatgggg gccacagtca acaacggcca ggtgaaaaat attgatccgg
     6961 gtatctcgac aatttcccgt ctgtggccag tggccaaccg tactcctcag ccagtgatca
     7021 ctgcgggaca aacgcagtat gcgaccgacg gaatcaatta cgtgaagaat gttatgcaga
     7081 cgcataacgg gttccagttc cacacccgcg tagacgagag cttcacagat tctctgaaat
     7141 tctatgtggt ctacaactgg gagcggatca atgatgaatc cgccatgaac aacatttatt
     7201 acaacccggg tgggaccgtc ccgtatccga cgcctcttta ctcttacggg catgcgcatt
     7261 acctgacagt ggacctcacc aaaaccttag gctcatcgct gacaaatgaa ctgattgcct
     7321 cgggggtcta tatgtcacag cccgagcagt ttggtaatcg cgccagggcc cagaccacgg
     7381 gtacagcgtg ggaggcggct ggttacagcc agggacacct aaatgtcggc cagacacagc
     7441 tgcctcgcat tatcagctac gatggcggta ttcctgcgtt cgcatttggc tatgttccgc
     7501 caacatcgca atttctgagg aagttctcct ggaacattgc tgacaatctg acgcgtgtat
     7561 acgggaagca cacctttaaa gccggtgtat atgccgagca aacagggaac agcaacgcga
     7621 cgcttggcag ccaactgaat ggcacgctca gtttcatgcg ctgggacggc tgctacatca
     7681 accagacatc gaaaacggcc aacccgccgg gtaccgcaaa tctgggtaat gtcattggca
     7741 atttcctgat tggctgtcct cttggctatg cgcaggacaa cagtgatcca gtcatcaacc
     7801 tgcggtttcg cacctttgag ggctacatca ccgatgaatg gaaggcaaca agcaatctga
     7861 cgctcaccct tggcatccgg ctctcacatc ttggtccctg gacagatgcg catggaattg
     7921 gcatagctgt ctgggacccg tcatccatct cgcaacacac gctctatcct gacaccgcgg
     7981 acagcacgac gtggccggga atcacctggc acaagagaaa cccaaggatt cccaatgctg
     8041 gggtgcccac acgggccctg ttctatgcgc cgcgcgtagg gttggcctat gatcttcacg
     8101 gtaatgggag gaccgttttc cgcggtggat ggggtatgta ttattcacat gacgcggccg
     8161 gcttttcggg tggaggggcg aacacctcga ctggcctggc aacatatacc actccgggca
     8221 atatctcctg caccttcgga caactcttca cgacgcagta cgttccctgc ggggcttatt
     8281 cctcgacgcc tactaccatt actccattct cagtgagcgc agtgaatcca aaagatgacc
     8341 gtatgccgct cacctataac tacaacttca cggtggacca gcaggggccg tggagcacac
     8401 tgtttgaaat tgcatatgtc ggcaaccaga gcacagattt gtcgagtctg ggtaatctcc
     8461 agaaccagaa tgtgattcca cttggcgcat tcttcaaacc agaccctctg acaggtcaac
     8521 tgaatccacc gagcagtatc cccaactcgg cagactacag gccatacccg aactaccagt
     8581 tcattaatgt tcccagccat attggatggg caaactacaa ctccctccag gcctcgtgga
     8641 acaagcagaa gggcgcgttg atctggggcg caaattacac ctggtccaag gcaatgggcg
     8701 tgcgcggcaa ctgggacact ggctctatag ctgaccccgt tgatccagag cacgattacg
     8761 gcatcgtttc ctttgaccgc ccccaggcgc tcaatttcac ctactcctac gatgtagggc
     8821 agaaatacaa cggcaataag attctcaaac aggtcataaa tgggtggcag atctccggaa
     8881 tcaactccat ccagagtggg ccggatctcg ctgttctgaa tggcaacact aattacaaca
     8941 tcaatggcgg tgtggactat acgtcgggtg ggaaaactgt aagtgttcca atcggcgcag
     9001 cggaatggct cggttcatcc gattacctgc ttcagcccgc cgttacctgc gatccgcgca
     9061 ctaacctgaa gaaggaccag tttgtcaatg gcggatgctt caccctgaca ccgcagggca
     9121 cgcaggggtg gtggaacttg cccgacaatc atggccctgc ttacttccgg tgggacctct
     9181 cggtttttaa gacctttaac attaccgagc gtcagaacat gcagttccgg gtttcaggct
     9241 ttaacttcct gaaccatcca ctcacctcat tcaacaacaa caacctgagt acgctcaacc
     9301 tgaatgtcgg aaactgtgct ggttgcaatg cgaccacacc cgagcaagcg atccagaatg
     9361 caacgatcac caatgccagt acttttggtt ccacagcgtt ccggacaggc gtgcgaatcg
     9421 ttgaactggg gttcaagtac aacttctagc gtgtggagcg gctgccgtcc agccgcttct
     9481 gcttaaattt gcagaggcct cgcctccgtg cctccctcgg gatggcctgt tgccatcccg
     9541 atttttttta gacctgaacg ttataattgg gcctttatgt gcctcattcc aatccatcca
     9601 atccagggag aagtgcaata atctatgttt agaccagttc tgattgctct gggattttct
     9661 gtcttttctg tgcttccgat tgccgcccaa tcggtccaga cttcaccccg ccagattgtg
     9721 gcagacctca aagatacaac cgggcctgta gatcactttt ttgatttgtc cgtcggctcc
     9781 gattatccgg gcaccctggt tcgcaatgac agtcaggcac agctcaagct cgccgtagac
     9841 gagcttggtt ttcgctatat ccgtttccat gccatctttc atgatgttct gggaacagtg
     9901 cagatcgaaa acggcaggac tgtttatgac tggagcggta ttgaccgcct ctatgatgac
     9961 ctgctcgcgc ggcacatccg gccattcgtc gaattgggtt tcacgccgca ggcattggcc
    10021 acctcgcaga acagtatttt ttactggcac ggcaacacct cgcatccgaa accgcaaggc
    10081 tggcaggacc tcgtgtcggc cttcatccgg cacattgaac agcgttacgg aaaagacgag
    10141 gtgcgcacct ggttcttcga ggtctggaac gagccgaacc tttccggatt ctgggaaaat
    10201 gccgaccagc aggcttattt tcagctctac gatctgacag caaggacgat caagtccgtg
    10261 gatcctgcgc tgcgcgtagg cggacctgca actgcaggag cagcgtgggt gcctgaattt
    10321 cttgcccatg tgaagcagag cggcgcggcc gtggattttg tctctacgca cacctacggc
    10381 gttgacggcg gtttccttga cgaaaatggg aagagcgaca cgaagctctc gccttcgccc
    10441 gacgccatca tcggcgacgt gcggcgcgtg cgcgagcaga tctctgcgtc accttttcct
    10501 ggccttccac tgtactttac cgaatggagc acgagctaca caccgcggga ctcagtgcat
    10561 gactcctata ttagtgcgcc gtatattctc acaaaactca aagcctgcca gggattggcg
    10621 cagggtatga gctactggac ttacactgac ctgtttgaag aaccaggccc tcctactgcg
    10681 ccgtttcagg gaggcttcgg gcttctgaat ccggagggca tccgcaaacc ggcttacttt
    10741 gcctacaaat atcttcatgc gttgcagggc agcagcattg ccacgaacga tgcgcaggcc
    10801 atgttggcca caagggatgg tgaccttacg gcagtactct gggactttga gcagcccacg
    10861 caaaaggtaa gcaaccggtc gtttttcaca aagcccattc cctcgcatcc atcggtgccg
    10921 gtgcaattga aggtaaaggg ccttgtcccg gatgcagcct atcaccttga aatacatcgg
    10981 accggatacc acgcgaatga tgcgtatacc gcctatctgg agatggggtc cccgaaagaa
    11041 ctgagcgcag cacaggtggc ctatctggac aggctcacgc aagacctgcc ggagacggac
    11101 cgcacagtgc acagcagccc cgacggctct ctggactttg tcctcccgat gaatagtaac
    11161 gacatcgtgc tggtaaagct ggagcgcgcc agaggggcga agtaactggt gcgtcgcttc
    11221 gcattcaaat ttcttccgac tggtcgtttt tcttgaaact tgaattggta ccaatccatc
    11281 caatcacaca ggagggcaat atgcctgaac ataataagaa agtggcgctc attacgggcg
    11341 caaaccgtgg gattggattt gagacagctc gccagctcgg acagaagcag gtcacgattg
    11401 tagtcggtgc aagaagtccc gaatccgcga aagaaaccgc cgacaaactc accgctcagg
    11461 gcatcgaggc gtatcctgtt gttctcgacg tcaccaaaga agcagaccgc aaagctgcgg
    11521 cgtcgtttat cgccaaaaaa ttcggcaggc tcgatatttt gattaacaat gcagggatcg
    11581 gcggtgatgg cggactcctg aatgctcaca ccatttccac gagccaggat gagcttcaga
    11641 gagtcttcaa tactaatctc ttctccgtcg ttgccgttac acgagagttc ctacccctgc
    11701 tcaagaagag tccggcaggc cgaatcgtca atctgtcgag catccttggc tccctgacgc
    11761 tgcaggcaat gcccaatagc ccgatcgcac cgatgaaggc ctttgcgtac aatgcatcca
    11821 agactgccct gaatcagttc accgtccacc ttgcggccga gttgaaggac acgaacatca
    11881 aggtgaactc ggtccatccc ggatgggtga agacggagct cggcacccag catgctcagt
    11941 tggaagtggc ggatggtgcc aagaccagtg ttgaatttgc cctgctcgga gcagatggcc
    12001 ccagcggtaa attcgttcat gacggacaag agctgccttg gtaagttctt ctctgtatac
    12061 cgcgcggagc gaagttgctt cccactccgc gtagcgtaca cgccgagcaa gatatcgttc
    12121 atcatccaca tggcacattt agagtgaaac tcccaacctg gcatttttgc catgtcaaac
    12181 tgttaagaca ggcaactggg acgattatga gcaaaggaac agaaactcgg aaaaggatta
    12241 tcacaaaggc ggccccactg tttaaccgaa aaggattcga gggctgctca atgcaggaca
    12301 tcgttgaggc cgtcggatta gaaaagggca gcctttatgg ccatttcgcc acgaaggaag
    12361 ctttggcgct tgctgcgttt gaatactcgt ggggagaaac atgcaccgca cgtctggcag
    12421 gaatggacac agcgaccaat gcaatcgcga aactcaggat tcacgtcgac aactttttgt
    12481 ctagtccgtc aattgcgggt ggttgtccgc tgctaaacac cattacagac agtgatgatg
    12541 gcaatcctgt cctcaaaaga gctgccagag aggctttgaa gcaatggcgc ctattgcttg
    12601 tgacgatcat tgaagaaggc caaagacggg gagaaataag gtccggggtt gttccggagg
    12661 aaatagctgc cttgctgctc tctctccttg aaggtgcgat ggccttagac cgtcttgata
    12721 agaaagcggg gtttcttcag aatgccgtac gtcatctgaa atcttatctc gattcgatat
    12781 cttcggagca gcgc
//
LOCUS       DASRBV010000057         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_207334, whole genome shotgun sequence.
ACCESSION   DASRBV010000057 DASRBV010000000
VERSION     DASRBV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207334"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFA02_02415"
     CDS             complement(<1..588)
                     /locus_tag="VFA02_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZU08730.1"
                     /translation="MSEMKKVLGVYGPGSNHWVGDGFPVRNLFPSNGLRDEINPFLML
                     DYAGPQYFKPSGKAHGVGEHPHRGFETVTIAYQGAVEHRDSGGHSGVIYPGDVQWMTA
                     ASGVVHEEMHEREFARNGGTFEMVQLWVNLPKAVKMSQPRYQGIKKEQIPSIDLGNGS
                     YARIIAGELSGTKGPALTFTPVNVFDVRLKAGTKAE"
     gene            complement(601..1065)
                     /locus_tag="VFA02_02420"
     CDS             complement(601..1065)
                     /locus_tag="VFA02_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU08731.1"
                     /translation="MKPRNQFSTTGTSGVHLWLVLWKAFHAVQAHALRNIAGLGMAYS
                     DFGVLEALLHKGPLTIGELGAKVLLTSGSMTAAIHRLEKRGLVTRRENAGDRRARVIE
                     LTPKGRALIRDAFQDHKRAMEFATAGLSSVDRQTLLNLLRQLGLSAAERSKK"
     gene            1294..2442
                     /gene="opgC"
                     /locus_tag="VFA02_02425"
     CDS             1294..2442
                     /gene="opgC"
                     /locus_tag="VFA02_02425"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HZU08732.1"
                     /translation="MHIPKPQRQPELDALRGLFLVWMTLTHLPTRFSDFVNSPIGYVS
                     SAEGFVFLSALLVGRVYMRQAAEGGAELRAALWKRSLRIYAYHLLMLALTFTVAAAYA
                     VRTHRAALYNLLNFYIAHPFVAVVGSVLLLYCPPLLDILPMYVIFLFFTPLVLTSAVK
                     RGWAGILATSMAFWLFAQFGLRDVVHNVIVHVTHLPIPLQETGAFNLFAWQAVWISGL
                     WLGAKSAVGEMPLRRIPGYVVTLSAVVCLFFIGVRHSWLGPHLTQQALGMKLDKWQIG
                     PLRVINLVTFTIVFYWLRKYVKRVVAIEPFLTLGKASLHVFCAHVFFVFVGLALLVND
                     VPELHGWPAAVLLAGTFAGLILVAVREVRNQRAKRLKREQQQIVQVFR"
     gene            2499..>3272
                     /locus_tag="VFA02_02430"
     CDS             2499..>3272
                     /locus_tag="VFA02_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZU08733.1"
                     /translation="MKGRGHGGCGIVYRGQWRTMMHAEAAALTDVGRLRSTNEDAYGL
                     LPEANFFLVCDGMGGAAAGEVASRMAVATALACVRRQTGKGPRALLEKLIAAANQRVF
                     SRAEREPSLHGMGTTLVSLLVRDDHVWIAHVGDSRCYRFRAGGLERMTQDHSLVEEQV
                     RLGQLTPAEAEKSPFRNMITRAVGTRASVAAEIREFPAQSGDVFLLCSDGLTKEVPEP
                     RIAEILGQDGDLQNLCQQLVDAANTAGGGDNITVVVVKVE"
BASE COUNT          626 a          866 c          990 g          790 t
ORIGIN      
        1 ttcggccttt gttccggcct tcaggcgaac gtcaaagaca tttaccggag tgaaggtcag
       61 cgccggtccc tttgtaccgg acagttcgcc tgcgatgatg cgcgcatagg agccgttacc
      121 gaggtctatg gaaggaatct gttccttttt tatgccctga tagcggggct gtgacatctt
      181 tactgctttt ggcaggttta cccagagttg caccatttca aaggtaccgc cgttccgggc
      241 aaattcccgt tcgtgcatct cctcgtggac aacgccggag gcggccgtca tccactgcac
      301 gtcgcccgga tagatgacgc cggaatggcc gccggaatca cggtgctcaa ccgcgccctg
      361 ataggcgatg gtgacggtct caaagccgcg gtgcggatgc tcgccgaccc catgcgcctt
      421 gccggatggc ttgaaatact gcggcccggc gtagtccagc ataaggaacg gattgatctc
      481 gtcccgcagg ccattggatg gaaagagatt gcgcaccgga aagccgtccc ctacccaatg
      541 gtttgatcca ggaccgtaga cacccagcac ctttttcatt tcagacattt caatactcct
      601 ttacttctta cttcgttctg ccgcgctcaa cccgagttgc cgcagcaaat tcagcagggt
      661 ctggcggtcc acggaggaca ggccggccgt tgcaaactcc atggcccgct tgtggtcctg
      721 aaaggcgtcg cgaatcaatg ccctgccctt tggggtcagt tcaatgacgc gcgcgcggcg
      781 gtcacctgca ttttcccttc gcgtaaccag tccacgtttt tccaggcgat ggatggccgc
      841 cgtcatggaa ccactggtga gcagcacctt cgcgcccaac tcgccaattg tcagcggccc
      901 cttgtgcaac agcgcttcca gcacgccaaa gtcgctatag gccataccca gtccggcaat
      961 gttccggagg gcatgcgctt gcactgcatg aaaggccttc caaagcacca gccagaggtg
     1021 gactccgctt gtgccggttg tagagaactg gtttcgcggc ttcatttatc ttgatatcaa
     1081 gatgtttcca gagagcaaaa ggattcatac ggtttcactt cgcggggaag attgtgggtc
     1141 ttccgggaat gtgtccggcc cggaatgacg gtggtttgcg tcactcatca tttgtctttt
     1201 caccgggcgc aactagcatc tctggaatgg tttctaatat agaaagacgg acttcgatga
     1261 caatatgcgg ctgtcgtttt acgtcttttt tatatgcata tacccaagcc ccagagacag
     1321 cccgaactcg acgccttgcg ggggcttttc cttgtctgga tgacactgac gcatctgccg
     1381 acgcggttca gtgactttgt caattcgccc ataggatatg tctcttcggc ggaagggttt
     1441 gtctttcttt cagcattgct ggtagggcgc gtgtacatgc gtcaggcagc agaagggggc
     1501 gcggagctgc gtgcagcttt gtggaagcgg tcgctgcgca tctatgccta ccatctgctg
     1561 atgctggcgc tcacgtttac agtagcagcg gcctacgctg tgaggacgca cagggcggcc
     1621 ctctataatc tgctgaattt ctacattgcg catccgttcg tagcagtggt ggggtccgta
     1681 ctgctgctct attgcccgcc gctgctcgac attctgccga tgtacgtcat ctttctgttt
     1741 tttacgccac tggtgctcac ctcggctgta aagcgcggat gggcaggaat tctggccacc
     1801 agcatggcgt tctggttgtt tgcccagttt ggcttgcgcg atgttgtcca taacgtgatt
     1861 gttcatgtga cgcacctgcc aattcctttg caggaaacag gagcattcaa tctttttgca
     1921 tggcaggcgg tgtggatttc cgggttatgg cttggggcca aatctgctgt gggagagatg
     1981 cctctgcggc gcattcccgg atatgtggtt acgttgagcg cagtggtatg tttgttcttt
     2041 atcggggtac gacatagctg gctggggccg catctgacgc agcaggcgct gggcatgaag
     2101 ctggacaagt ggcagattgg gccgctgcgc gtcatcaatc tggtgacgtt tacgattgtc
     2161 ttctactggc tgcgaaaata tgtaaagcga gtggttgcaa ttgaaccgtt tctgactttg
     2221 ggaaaggcct ctctgcacgt cttctgcgcg catgttttct ttgtgtttgt aggactggcg
     2281 ctgctggtca acgacgtgcc cgaattgcac ggctggcctg ccgccgtact gcttgcaggc
     2341 acttttgcag ggttgattct cgtcgctgtc cgcgaagtgc gtaaccaaag ggcgaagcgg
     2401 ctcaagcgcg aacagcaaca gattgttcag gtattccggt agccattccc ggcctgattg
     2461 agcggatgtg gcccacgttc tttcatgcaa aaatcagcgt gaaaggacgg ggtcacggtg
     2521 gttgcggcat cgtgtatcgt ggtcaatggc gaaccatgat gcacgcggaa gcagcagcac
     2581 tgacagatgt aggccgtctt cgctcgacca atgaagatgc ctatggtctt ctgcctgagg
     2641 cgaatttctt tcttgtctgc gacggaatgg gcggcgccgc ggcaggcgaa gtcgccagcc
     2701 ggatggcagt ggccacggcc cttgcatgtg tcaggaggca gacggggaaa ggaccacgag
     2761 cgcttcttga aaagctgatt gccgcggcca accagcgggt gttttcgcgc gctgagcgtg
     2821 aaccgtcact gcatggcatg ggcactacgc tggtctcact gctggtgcgc gatgaccatg
     2881 tgtggattgc gcatgtgggc gacagccgtt gctaccgctt ccgcgcggga gggctggaac
     2941 gcatgacgca ggaccactcg ctggtggagg aacaggtgcg gctgggacag cttactccgg
     3001 cagaggcaga gaagtctcca tttcgcaata tgattacgcg agcggtgggg acgcgcgcga
     3061 gtgtcgctgc ggagatacgg gaatttccag cacagtccgg agatgtattt ctgttgtgct
     3121 ccgatgggct gacgaaggaa gtccctgagc cgcgcattgc ggagattctg gggcaggatg
     3181 gagatttgca aaacctctgt cagcagttgg tggatgcggc caacacagcc ggcggagggg
     3241 acaatatcac ggtggttgtg gtgaaagtgg ag
//
LOCUS       DASRBV010000058         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_559707, whole genome shotgun sequence.
ACCESSION   DASRBV010000058 DASRBV010000000
VERSION     DASRBV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559707"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFA02_02435"
     CDS             complement(<1..378)
                     /locus_tag="VFA02_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZU08734.1"
                     /translation="MKIHEYQAKDILARYGVAVPRGEMANTLDEALDVAKKLFSGGAN
                     GVVVKAQIHAGGRGKGGGVKVAKNLEEAEHYAKQILGMQLVTHQTGPQGQKVQRLLIE
                     ETAAIDRELYLGIVLDRAAAKLVF"
     gene            complement(552..923)
                     /locus_tag="VFA02_02440"
     CDS             complement(552..923)
                     /locus_tag="VFA02_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZU08735.1"
                     /translation="MSIAEVFSELSAREPIFHRPEFGTSRADFERMTTADFWEVSASG
                     RRYSRDEVLNVLEKRYSAPYEDVWETNDFKCQQLAPDVYLLTYTLVQDHQRITRRATI
                     WQRTSERWKVAYHQGTIVQDE"
     gene            complement(910..1728)
                     /locus_tag="VFA02_02445"
     CDS             complement(910..1728)
                     /locus_tag="VFA02_02445"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZU08736.1"
                     /translation="MSEVRLQKIIAQAGIVSRRKAEELILAGRVQVNGQTVTELGTKA
                     DPQHDHIRVDGKLLHGPERHRYYMMNKPKGYVTTVSDPEGRPTVMQLLRGAGERVYPV
                     GRLDYNSEGLLLLTNDGELANALTRASSRVRKTYLVKVSRKPSPEAIQQLRSGIMIER
                     DKPGSRQGRVMTAPAEIRLFRDAENPWYEVTLIEGRNREIRKMFEEIGHHVEKIRRVA
                     YGPLELDISPGEVRELSGKEVEELRRSASGKQSEKSPRPPRRTTKGRGAQIEHR"
     gene            complement(1725..2669)
                     /gene="scpB"
                     /locus_tag="VFA02_02450"
     CDS             complement(1725..2669)
                     /gene="scpB"
                     /locus_tag="VFA02_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZU08737.1"
                     /translation="MSLKAKIEAVIYAAEEPVTLAQLAALFADEALEEKRQREDASAS
                     EADTDAPVIPVEEVAPLTVEEAAVMAEQASEESAPSAEDEKKAARQRDREVREELRRI
                     IAELIGDYANNDRGMEIREIAGGYRVATKPEYHDAVRAFVKSLKPPMKLSLQALETLA
                     VIAYKQPITAPEVSEIRGVDSAGVIGSLISRKLVTTAGRKQVIGRPILYKTTKEFLLR
                     FGLKDLNELPSMEEFEKMAAGELAEPGELPFEGQTTGTPNSEGILETPDEGPGPQAAV
                     EVTEELEEEEEDVPGVAVDSQKDEEEAFEASSAEAGQP"
     gene            2789..4012
                     /locus_tag="VFA02_02455"
     CDS             2789..4012
                     /locus_tag="VFA02_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU08738.1"
                     /translation="MPATAPATAFARIGHLASQRRVHEAFGWLHLHEQQIMRWQRELV
                     AVPAPPFAEGPRAEWLCARFRELGLADVAIDGIGNAVGVLRGENPAAGCVVLSAHIDT
                     VFPAETPIQPQLEDTKLFAPGACDNGAGVAALLAIASALRHGHIRPAGDVVFAGNVGE
                     EGEGDLRGMRWIYEHAAWRDQIAAHIVLDGAGHEVAVTRALGSRRYLATVSGPGGHSW
                     TDAGRANPIVVLSRAIVRLGDFNLNGPPRTTLNVGMMEGGTAINAIPELAMARFDARS
                     TDAGQLIRLEVELHRAIEDAVMDANKDIRKELALRFAIQKIGDRPAGVLPENSSLLEN
                     LRAVDRHLGVRTETRIASTDANIPLALGVPALSMGAGGEGGGIHTRAEWYDARGRELG
                     LRRILLLLLACGHGA"
     gene            4083..>5007
                     /locus_tag="VFA02_02460"
     CDS             4083..>5007
                     /locus_tag="VFA02_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU08739.1"
                     /translation="MTPADAIYYHGNVLTGLSLEEAHPQRVSALAIRNGVIVATGSDA
                     DIQRWKGPATLMVDLDGAFVMPGFNDAHAHLGEAGRIKLSIDLTCVKSLSEMEQRIRA
                     AAKSAAPGAWLTGGGWDHTLWASKKLPTKSDLDAVTAGHPAFLVRVDGHIAIANSAAL
                     HAAGITKSTPDPQGGKIDRDAHGEATGIVRETAQALVESHIPPPSPELRRRGIELALA
                     DAVEHGVTSVQDFSDHEDFLVYEQLEREGRLPVRISEWIPFDLSVEQLKQYQASHLQS
                     DPMLHTTMLKGFMDGSLGSRTAALKAPYSDDP"
BASE COUNT          939 a         1483 c         1452 g         1133 t
ORIGIN      
        1 aaagaccagc tttgccgcgg cacggtcgag tacgatgccc aaataaagtt cgcggtcaat
       61 cgcggccgtc tcttcaatca acaaccgctg gactttctgt ccctgcggac cggtctggtg
      121 cgtgacgagc tgcatgccca ggatctgctt tgcgtaatgc tcggcttctt ccaggttttt
      181 tgcgactttt acaccgccac ccttgccgcg gcctccggcg tggatctgcg ccttgactac
      241 gacgccgttg gctccgccgg aaaagagttt tttggccaca tccagcgctt catcgagcgt
      301 gttggccatc tcaccccggg gcacggcaac tccgtacctg gccaggatgt cctttgcctg
      361 atattcatgg attttcatag cttgtctggt ggttcaaagg ccgcagattg acccgttgcg
      421 cggaagctcc gtgcagctac gggtgaattg acggctaacc ttttatagta caggggccag
      481 cggcgggttg tcaggtttgg tgcggtgatt gattggcaga atccccattg ccgaataata
      541 tttcgtcggt atcattcgtc ctgcacaatc gttccctgat gatacgcaac cttccaccgt
      601 tcagaagtgc gctgccagat cgtagcgcgt cgagtaatgc gctgatggtc ctggacaaga
      661 gtataggtca gaagataaac gtcaggcgca agttgctggc acttgaagtc gttcgtttcc
      721 cagacgtcct catacggtgc cgagtagcgc ttctccagca cattaagcac ctcgtcgcgg
      781 gaataacgcc tgcccgacgc actgacctcc cagaagtcag cggtcgtcat gcgctcgaaa
      841 tctgcgcgac tggtgccaaa ttccgggcgg tgaaaaatag gttcccgggc agacaattcc
      901 gaaaagacct cagcgatgct caatttgcgc gcctctacct tttgttgtcc tccgtggtgg
      961 tctcggcgat ttttctgact gctttccgct cgcgctcctc cgcagttctt ctacttcctt
     1021 ccccgataac tcccttacct cgcctggaga gatatccagc tccagcgggc cgtaggcgac
     1081 gcggcggatc ttctctacgt gatgcccgat ctcctcgaac atcttgcgga tttcgcggtt
     1141 gcggccttca atcagcgtca cttcgtacca agggttttct gcgtcgcgga atagcctgat
     1201 ttctgccgga gccgtcatca cacggccctg ccttgatcct ggtttgtcgc gttcgatcat
     1261 gattccggag cgcaattgtt ggattgcttc ggggctgggc tttctgctca cttttaccag
     1321 ataggtcttt cttacccgcg acgaagcgcg cgtaagagca ttggccagct caccatcgtt
     1381 ggtcagcaag agaagacctt cgctgttgta gtcgaggcgt cctacgggat agacacgctc
     1441 gcccgctccg cgcagaagct gcatgaccgt gggccgtcct tccggatcac tcaccgttgt
     1501 gacgtagcct ttcggcttgt tcatcatgta gtagcgatgg cgctccggcc catgcagcag
     1561 cttgccgtct acgcggatgt ggtcgtgttg gggatcggcc tttgtgccca gctcggtgac
     1621 ggtctgaccg ttgacctgga cgcgtccggc aagaatcagc tcctcagcct tacggcgtga
     1681 gacgatgcct gcctgcgcga tgattttctg taagcgaacc tcgctcatgg ctggcccgct
     1741 tcggcggaag acgcttcgaa cgcctcttcc tcgtcctttt gggagtcaac agcgacgccc
     1801 ggaacgtcct cttcttcctc ttccagctct tcggtcactt ccacggcggc ttgtggtcct
     1861 ggcccctcgt ccggggtttc gagaatgccc tcagagttcg gtgttcccgt tgtctgaccc
     1921 tcgaatggaa gctcacccgg ctcggccagt tcgcccgcgg ccatcttctc gaattcttcc
     1981 attgaaggca gctcgttgag gtctttcaat ccaaagcgga gcaaaaattc tttcgttgtt
     2041 ttatagagaa tcggacgtcc gatgacctgc ttccggcccg ccgttgtgac cagtttgcgg
     2101 ctaataagtg acccaatgac gcccgctgaa tccacgccgc gaatctcgct gacttccggc
     2161 gctgtaatcg gctgcttgta agcgatgacg gccaaagttt ccagcgcctg caacgaaagc
     2221 ttcattggag gcttcaggct ctttacgaat gcacgcacag catcgtggta ttcaggcttc
     2281 gtggcgacgc ggtatccgcc ggcaatctcg cggatttcca tcccgcggtc attattggca
     2341 tagtcgccga tgagttccgc gatgatgcgg cgcagttcct cgcgcacctc gcggtcgcgc
     2401 tgacgggccg ccttcttttc atcttcggca gagggtgcgc tctcttcgct ggcttgttcc
     2461 gccatcactg ctgcctcttc cacggtcagc ggagcaacct cttctaccgg aatgaccggg
     2521 gcatctgtgt cggcctcact tgcagatgcg tcttcgcgct ggcgcttttc ttcaagcgcc
     2581 tcgtcggcaa aaagagctgc gagctgcgcc agggttaccg gttcttcggc ggcgtaaatg
     2641 actgcttcga tctttgcttt cagactcatg ttcaggggcg gctttcttag atactaccga
     2701 agcaagggct gtttggtgaa aatcttcgcc tgtccttcac ccatcgggag ccgggaaaaa
     2761 gcagcgatgg gatgctacgc tgggatttat gcctgctact gctccggcga ccgcatttgc
     2821 gcgcatcggc catctggcct cgcagcgccg tgtgcatgag gcatttggct ggctgcatct
     2881 gcatgagcag caaattatgc gctggcagcg ggagctggtt gctgtgcctg ctccgccatt
     2941 tgcagaaggt cctcgggcgg aatggctttg cgcgcgcttc cgcgaactgg gcctggcaga
     3001 cgtagccata gatggcattg gcaacgcggt cggcgtcctc aggggagaaa atccggcagc
     3061 cggctgcgtg gtgctttctg cgcatattga tacagtgttt ccggcagaga cgcccatcca
     3121 gccgcaattg gaggacacaa agctgtttgc tcccggagca tgcgacaatg gcgcgggtgt
     3181 ggcagcgctt ctggccatcg cttctgcctt gcgccatgga catatccggc ctgcaggcga
     3241 tgtagttttt gcaggcaacg taggcgaaga gggcgaaggc gacctgcgcg gcatgcgctg
     3301 gatttatgaa catgccgcat ggcgcgacca aatcgcggcc catattgttc tggatggcgc
     3361 gggacacgaa gtggccgtaa cccgcgcgct ggggagcagg cgttatctgg ctacggtaag
     3421 cggccctgga ggacactcct ggaccgacgc cggacgtgcc aatccgattg tggtattaag
     3481 cagggccatt gtgcgcctgg gtgactttaa cctgaacggc ccaccgcgca ctacgctgaa
     3541 tgtcggcatg atggagggcg gaacagccat caatgccatt ccggaactcg ccatggcccg
     3601 cttcgatgcg cgctcaacgg atgccgggca actgatacgg ctggaagtgg aactgcatcg
     3661 cgcgatagag gacgcagtca tggatgccaa caaagacatc cggaaagaac tggcgcttcg
     3721 ctttgcgatt caaaagatcg gcgaccggcc cgctggagtg ctgccggaaa actcttcttt
     3781 gcttgaaaac ctgcgcgcgg tagacaggca tttgggagtt cgcacggaaa cgcgcattgc
     3841 ctcaacggat gcaaacattc ctctggccct gggggtaccc gcgctttcca tgggagcagg
     3901 cggtgaaggc ggaggcattc atactcgcgc cgaatggtat gacgcacgcg gacgcgagct
     3961 tgggctgcgg cgcattctac tgcttctgtt ggcctgcggc cacggcgctt gatagactcc
     4021 ggtgcgtgaa gatactagcg agtttgcttg ccgctttcgt ttccatggca ggagcccaga
     4081 ccatgacgcc cgccgatgct atctactacc acggcaatgt tcttaccggc ctaagtctgg
     4141 aagaggcgca cccgcagcgg gtttcagctc tggccatccg gaatggcgtg attgttgcca
     4201 ccggaagtga tgctgacatc cagagatgga aaggcccggc cacactgatg gttgacctcg
     4261 atggcgcatt cgtcatgccg ggattcaacg atgcccatgc gcatctgggt gaggccggac
     4321 gcatcaagct ctccattgac ctgacctgcg tgaagtctct ctcagagatg gagcagcgca
     4381 ttcgtgccgc cgcaaagtca gctgctccag gcgcatggct caccggaggc ggatgggacc
     4441 acacgctctg ggcatccaaa aagctgccaa cgaaaagtga ccttgatgcc gttacggcag
     4501 gccatccggc atttcttgtt cgcgtagatg gacacattgc catcgccaac tccgcagctc
     4561 tgcatgccgc cggaatcaca aagagcacgc ctgatccaca gggaggaaaa atagaccggg
     4621 atgcgcacgg cgaagctacc ggcatcgtcc gcgaaacagc gcaagcgctg gtcgaaagcc
     4681 acattcctcc gccaagcccg gagttacgcc ggcgaggcat tgagcttgcg ctggccgacg
     4741 ccgtcgagca tggtgtgact tcggtgcagg acttctcaga ccacgaagat ttcctcgtct
     4801 acgaacaact cgagcgcgaa ggcaggctcc cggtccgcat cagcgagtgg atacccttcg
     4861 acctttcggt agaacaactg aagcagtacc aggcgtcgca tctgcaaagc gacccgatgc
     4921 tgcacacgac gatgctcaag ggctttatgg acggctcgct tggttcacgc acggccgcgc
     4981 tgaaagcgcc gtatagcgat gatcccg
//
LOCUS       DASRBV010000059        16036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_611192, whole genome shotgun sequence.
ACCESSION   DASRBV010000059 DASRBV010000000
VERSION     DASRBV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 16036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16036
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611192"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFA02_02465"
     CDS             complement(<1..238)
                     /locus_tag="VFA02_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZU08740.1"
                     /translation="MAERLYYTDPFLRSFSARISEIHELSRAEGQSVWKVALDRTAFY
                     PASGGQPHDLGTLTATSRSGAVLEVPVIAVEEDGH"
     gene            complement(500..2017)
                     /gene="guaB"
                     /locus_tag="VFA02_02470"
     CDS             complement(500..2017)
                     /gene="guaB"
                     /locus_tag="VFA02_02470"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZU08741.1"
                     /translation="MIEHPVPEALTFDDVLLVPAYSEVVPALVSTQTRLTNRIRLNTP
                     LVSAAMDTVTESRLAIAMAQQGGIGIVHRNLTIEQQAAEIDKVKRSESGMIVDPVTMD
                     PGQLISDALEVMRKYKISGVPVTKGKKLVGILTNRDLRFETRTDIPIGDVMTKENLIT
                     VPVGTTLEEAERILHQHRVEKLLVVNDQYELKGLITVKDIQKKLKYPNAAKDEQGRLR
                     VGAAIGATGDFLERAAEMVRYRVDVLAIDSAHGHSSRVLEAVREVKKRFPEVDLLAGN
                     VATYDGAKALMQAGADAVKVGIGPGSICTTRMVTGAGMPQVTAISEAYRAGREFNIPI
                     IADGGIKYSGDIAKAIAAGASSVMIGSLFAGVDESPGETILYQGRSFKAYRGMGSLSA
                     MAQGSGERYFQSSRDIEQVSERSVVTRDGNGQNRLAKFVPEGIEGRVPHRGPLEAMVY
                     QLVGGLRSGMGYLGCATIEELQTRSRFVRITNAGLRESHVHDVIITREAPNYHVE"
     gene            2175..2864
                     /locus_tag="VFA02_02475"
     CDS             2175..2864
                     /locus_tag="VFA02_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08742.1"
                     /translation="MSRFWFRFAWLLLAFGGMAQARADDMNCNGQPVTTHKFQSQPGD
                     VLHPQHLEITRPYSGTGKLEVSVCNADLRIRPDSHAKELKLLVDMKSQTDGHPAEDYI
                     HTLDVTPDHGRIHLKFTKAAHASVTLVVPMDAGSHNEFDLGRGQLVFDAAGTAGQREI
                     NVGMGDMKLIVDGDQDYSGMEVNIGMGSLHDHRPGGHDGHFVVSRNYSGTGNGPLEIN
                     VGMGSLDINQE"
     gene            complement(2923..3858)
                     /gene="trxB"
                     /locus_tag="VFA02_02480"
     CDS             complement(2923..3858)
                     /gene="trxB"
                     /locus_tag="VFA02_02480"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZU08743.1"
                     /translation="MSDNIRDTVILGSGCSGLTAAIYTARANLKPLVLEGHEPGGQLS
                     ITTLVENFPGFPEGIQGPQLIENMKQQASRFGAELRMAHLVSADLSKRPFALNLGSET
                     IHTRTLIIASGASARWLGLPSEQALIGHGVSSCATCDGFFFSGKEIAVIGGGDSAMEE
                     ALFLTRFATKVFVIHRRDHFRASRIMLDRAIAHPKIQFVTNTTVEEVLGVEEKEVKGL
                     RTRNVLTGEESILQVSGMFLGIGHDPNAKMFAGQLDLDEDGYIKTQNYVHTRVPGVYA
                     CGDVQDRRYRQAITAAGTGCMAALEVEKYLEEHGR"
     gene            3953..4153
                     /locus_tag="VFA02_02485"
     CDS             3953..4153
                     /locus_tag="VFA02_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HZU08744.1"
                     /translation="MADLTLVYGLRLLPADEIAEVGEAPIQLKNGNTARVTMHILEGT
                     REQIEAQLRQSIDAFFDFFPEI"
     gene            4294..7179
                     /locus_tag="VFA02_02490"
     CDS             4294..7179
                     /locus_tag="VFA02_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZU08745.1"
                     /translation="MFRCLRKWVVLFSLLLVSQAGLCGGPRWVAGPAYFDPAVKGQVV
                     HWPNGTVSYYLDQGALSPYVDLGQVANMIAAAAAVWSQVPTAAVSITQGGTLDEDVNG
                     SNVSANPDGSVNVPSDVQPSATNKPVAIVFDADGSVINAFLGAGASDPSDCRDNGVVT
                     FVDNIGTDAAIRHALMILNGLCAWNSSQLTVMQYQVMRGFGRVLGLDWSQTNEENFAT
                     HTITSDDLVGWPVMHPVEYLCQFSGTFCSPDLTALRYDDIAAINRLYPVTASNVSAFP
                     GKLLTAAATVSVQGTIHFRNGQGMQGVNVVLRPINAGTGLPDVRYTVTAVSGRFFQGN
                     APNPVNGASAASNYGSDDPSLEGWFDLSGVPLPPGTNIADYQLSFEPINPLYTANYSV
                     GPYTTGQVTPSGTMPSIMLYGLTAGKNFTQDVVIQDSADEEYTGHDGTETAPARIPAG
                     GEWTGRITGYGHTAWFDWQVRPNRELTVEAVALDESGAPTLNKAQVVLGAWNGSDAPG
                     GPPVSATTQAFNGAQTGLTTLSVATVAAGHLRLGIADARGDGRPDYLYHARLLYADSV
                     MPTRISLSGGPIIIRGTGFRPNAIVTVNGVRAAVTGVTSTEITAVAPPANGKTGVVLL
                     TVSDPQTLAVTAIQDGLSYDALGGDALGIVTAPYGQTNLGVWTPFTVKALAADGQTPA
                     AGVIVTFSVTNGTAALGCGQAACSVLSGGDGLATIKVAANSTSPAQVTASLSDGASIM
                     TEFTGSGTPMIAALTPYLYVAAGASFSWDPQAQTLLNGVSMPGQPVLWSASAGATAGT
                     SSTLSDSSGTATGQINISPLAAGADVPVYACLSGNVQCAPFHIYSVHPEIAQLSAVSG
                     VGQTISASETPVPVVLRVTDAVGHPMAAGTVTFYETLKQWTPDCPPQGRCPSAPVIAT
                     QIVPAVSGADGLITLNPLTGNGVPTRLYVTAVTGNSASLNFEIEQHP"
     gene            7262..8668
                     /locus_tag="VFA02_02495"
     CDS             7262..8668
                     /locus_tag="VFA02_02495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU08746.1"
                     /translation="MAAEQIPAIREGRRPRVLILGGGFAGLHAAKGLAKLPVDITLVD
                     RRNHHTFQPLLYQVALAVLSPADIATPIRGILRRHKNVEVIMDEAIQIDKERHIVRMK
                     SGAEMEYDYLVVATGSTHSYFGHDEWAQVAPGLKTVEDATEIRRRVLLAFELAERQML
                     ETGSHPPLNFVVIGGGPTGVELAGAISDIAKLYMRHDFRHIDPSKARVILLEGGPRIL
                     TAYPEDLSKKAVEQLNDLGVEVHTGAQVTDVQPGYVVVGGQRLDAVVTLWAAGVQASP
                     LGKLLGVETDRRGCVMVNATLNPSGHPEIFVCGDLAHFEQDGKQVPGVAQPAMQMGDH
                     VARMIEQDLKGTARSGFHYFDKGDMATIGRMAAVAKIEWPFKAHWSGFMAWITWLTIH
                     IFFLIGFRNRIFVFWEWAWTYFTFTHGARLITGSQVLPGWTEQQGVEQEEVSKPLDPT
                     SPGFRNHHGEVAAAGEKR"
     gene            complement(8713..9567)
                     /locus_tag="VFA02_02500"
     CDS             complement(8713..9567)
                     /locus_tag="VFA02_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08747.1"
                     /translation="MAVSPFSSLARSMEPWLWLLVLIGYLRARGHLLSLFFGIFLGIR
                     ALTAFVLTVLFLRHPEPGQASVLPPIAFYTSWTGFLAGFVVLLFAFKEIFRHILAPLP
                     GLLHMGGIVFRWVALVSLLLSCAVVLPSLLHPHGSHIEFVLLQIATGICALELCLVAF
                     LVVAARALGFSAKSRIFCICIGLMALALADIAGFSASLAHAASVAVYICYSVTILCLV
                     VWIGFFLSPEPVREFAHAPMKALLRWNDLALALHPPEEPAEERTGFLHDVESMVDRVL
                     ARNASKAN"
     gene            9905..12511
                     /gene="gyrB"
                     /locus_tag="VFA02_02505"
     CDS             9905..12511
                     /gene="gyrB"
                     /locus_tag="VFA02_02505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZU08748.1"
                     /translation="MEHSSPEKNGRKSTNDLEKNYTGENIKVLEGLEAVRLRPAMYIG
                     STGEMGLHHLVYEVVDNSVDEALAGYAKKIDVVIHVDNSITVIDDGRGIPVDEMTLPG
                     GEKLPAVQVVLTKLHAGGKFDSSTYKVSGGLHGVGVSCVNALSEEFDVEIWRDGHTYE
                     QDYERGIPVSKLRQTGTSRKRGTKVHFLPDKTIFTTTEYNYDTLAQRLRELAFLNKGL
                     EITLSDERTTDPKTGEAKRAEFKYVGGIAEFVKHLNKGKQVLHDKPIVMEAVRDGVDM
                     EIALQYNDGYSETVFSFANNINTVDGGTHLSGFRTALTRTINAIGQQMGLFKDVKENL
                     SGDDVREGLVAVISVKLPQPQFEGQTKGKLNSDIAGTVQALVNEKLGMFLEQNPPVAR
                     RIINKAIEAARAREAARKARDLTRRKGALDSGGLPGKLADCSERQPDRCELYLVEGES
                     AGGTAKQGRDRRFQAILPLKGKILNVEKARYDKMLGHEEIRAMITALGTGIGKDDFDA
                     SKLRYGKIILMTDADVDGSHIRTLLLTFFFRHMNELIKRGHVYIAQPPLYRIKKGKFE
                     QYIKDDREFVRVMVKRASDGMIVRHGENATKLEGAALTKFIGQLNEYLGFFDKVNKRI
                     RNEEVTGLFAKLFAHEGKEPVKRADFEGDAKTVPAKLTALYDELKKIEKRYQFKSLDK
                     PVYDEEHQTWSLLFHDAQGAERKIDWVFASAPETRQMLAKYAQIKDAIEPPFLIEYAG
                     KAAVAEPDPEENEGETTGEAEAGKPAPKRAGRASHDPVEKQSPRELFEYVIEQGRRDY
                     QVQRYKGLGEMTSSQLWETTMDPERRTLLKVKLEDIAETETIFTTLMGEDVEARRKFI
                     EDNALDVKNLDI"
     gene            12855..13537
                     /locus_tag="VFA02_02510"
                     /pseudo
     CDS             12855..13537
                     /locus_tag="VFA02_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14219..15697
                     /gene="ltrA"
                     /locus_tag="VFA02_02515"
     CDS             14219..15697
                     /gene="ltrA"
                     /locus_tag="VFA02_02515"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZU08749.1"
                     /translation="MQRKLHQWSRNHPEDSYRDLWNWTTDIRNLRCAWRQIAQNQGRR
                     TAGVDGMTVAGICEAIGVGLFLEQIRDDLKSGRYKPSPSRRKLIPKPRQPGKFRPLGI
                     PTVRDRVVQCAVKNILEPIFEADFWHVSYGFRPGRGCHGALEHIRLSMCPKVKAEDGR
                     RQKTPYQWVIEGDIKGCFDNIDHHQLMERVRTRIIDGKVTTLITQFLKAGVLADGFLL
                     PTDTGTPQGGVISPLLANIALGVIEERYERWTHHRRKIQARRKSDARTAAMWARMGDR
                     KAGRPVFFPIRYADDFVVLVSGTCEQAEQEKAELARYLKDTMKLELSLEKTRVSDLKE
                     GFQFLSQRVRYKWHRRFGYMPRIEIPTSKRADLRYRVKQLTTRATTQWSLSELLQKLN
                     PILRGWGNYYRFCTGASRQFSALDFYVGDRIWRWLMKKHKHLHRKRRRLVRVPSLLRP
                     TRKVWREGRTEQFLLSMLRVERFRRGWMRTPAYICVPGEPDA"
BASE COUNT         3615 a         4267 c         4877 g         3277 t
ORIGIN      
        1 cgtgtccgtc ttcttccacc gcgatgacag gcacctccag taccgccccg ctgcgcgaag
       61 tggccgtaag cgttccgaga tcatggggct gacctccgct ggccgggtaa aacgccgtgc
      121 ggtcgagggc gaccttccag acagattggc cctctgcgcg cgaaagctca tggatctcac
      181 tgatgcgggc gctgaagctg cggaggaacg gatcagtgta atagaggcgt tctgccatgc
      241 ttaaaatcag tgtacccgag cggcgaatcc atccgcgatg ggacatttta ctcacgcacg
      301 gccggtgaag gaaagcggga gtatccctca aatctcgggc ggagtgtcat caccatatag
      361 atataattgc ccacggcgcg ggtttctgcc caaccgagaa gatggaagaa aagaaaaaca
      421 gcccaagcca gcagaatttc atcatggcgt acacgaaagt gcagccagta tagcttgcgt
      481 gcctcaggtt gtgcaggact tattccacgt gataattggg cgcttcgcgg gtaatgatta
      541 cgtcgtgaac gtgactctcg cgcaatccgg cattcgtgat gcggacgaaa cgcgacctgg
      601 tttgtaattc ctcgattgtg gcgcatccca gatagcccat tcctgaacgc agcccgccca
      661 caagctggta gaccattgct tccagcggcc cacgatgcgg cacacggcct tcaatgcctt
      721 cgggaacaaa cttggcgaga cggttctgtc cgttgccatc gcgggtaacc accgaccgct
      781 cggaaacctg ctcgatgtcg cggctgctct ggaaataacg ctcgccggac ccctgcgcca
      841 tcgcagaaag cgaacccatt ccacgatagg ccttgaagga gcgcccctga tacaggatgg
      901 tctcgcccgg actttcgtcc acaccggcaa agagcgaccc gatcatcacg ctgcttgcgc
      961 ccgcggcaat ggccttggca atgtctccgg agtacttgat gccgccatcg gcaatgatgg
     1021 ggatgttaaa ttcgcgtcct gcgcgatagg cttcgcttat ggctgtaacc tgcggcatgc
     1081 cagcgcccgt taccatgcgc gtggtgcaga tggacccggg accgatgccg acttttacgg
     1141 catccgcgcc tgcctgcatc aatgcctttg caccatcgta agtggccaca tttccggcga
     1201 gcaggtctac ctccgggaag cgctttttta cttcgcgcac agcctcaagc acgcgcgacg
     1261 aatgtccatg cgccgaatca atcgccagaa catccacacg ataacggacc atctcggcgg
     1321 cgcgttccag aaagtctccg gtagcgccaa tcgcagcccc aacgcgaaga cgcccctgct
     1381 cgtcttttgc ggcgtttgga tatttgagtt ttttctggat gtctttgacg gtgatgaggc
     1441 ctttgagttc gtactggtca ttgacgacga gcagcttttc cacccggtgc tggtgcagga
     1501 tgcgttcggc ctcttccagc gttgttccca ccggcacggt aatgaggttt tccttcgtca
     1561 tcacatcgcc aatggggatg tcagtgcgcg tctcaaagcg caggtcgcgg ttggtgagga
     1621 taccgacgag cttctttcct ttggtcaccg gaacaccgga aatcttgtat ttccgcatga
     1681 cttcgagcgc atcagagata agctgcccgg ggtccatcgt gacggggtcc acgatcatgc
     1741 cgctttccga gcgctttacc ttgtcaatct ccgcggcctg ctgctcgatg gtcagattgc
     1801 gatgcacgat gccgattccg ccctgctggg ccatggcaat cgccagacgc gattcggtca
     1861 ccgtgtccat ggccgcgctc accagaggcg tattcagccg gatgcggttg gtaagccgcg
     1921 tctgcgtgct gaccagtgcc gggaccactt cactataggc aggaaccagt aaaacgtcgt
     1981 caaatgtaag cgcttcgggg accggatgtt caatcattgt gtttgtcagg tgcgaagggg
     2041 accttcgcgg gttgtagggc tgttcttatg cacaagtagt tcttttgatt gtagagagat
     2101 tgagaataca gggcaaattc cgcagtggga acgaattcgg gccggttttc gtactgtcct
     2161 ttcaagagaa agatatgagc agattctggt ttcgttttgc ctggcttctg ttggcatttg
     2221 gcggcatggc gcaggcccgc gccgatgata tgaattgcaa cgggcagccg gttacaacac
     2281 ataaatttca atcgcagccg ggagatgttc ttcatccaca gcatcttgag atcacgcggc
     2341 cctattcggg gaccggaaaa cttgaagtaa gcgtctgcaa tgccgatctg aggattcggc
     2401 ccgattcaca tgcaaaggag ttgaagctgc tggtggacat gaaatcccag acagatgggc
     2461 acccggcgga ggactatatc catacgcttg acgtgacccc ggaccatggc agaatccatc
     2521 tcaagttcac aaaagcagcg cacgccagcg tgaccctggt cgtgcctatg gatgcaggct
     2581 ctcacaatga atttgacctg ggccggggcc agcttgtttt tgatgctgcc ggtacggcag
     2641 gtcagcgcga gatcaacgtc ggcatgggcg acatgaaatt gatcgtggac ggcgaccaag
     2701 attactccgg aatggaagtc aatatcggca tgggatcact gcatgaccac cgtcctggag
     2761 ggcacgatgg ccacttcgtc gtcagccgga attacagcgg caccggaaat ggcccacttg
     2821 aaatcaacgt cggcatgggc agcctggaca tcaatcagga atgacccccg aatcagtagg
     2881 caattcctac atctttcgat tccgggatgc ggcgctgcca tgttatcgcc cgtgttcttc
     2941 cagatacttc tccacctcta atgcggccat gcagccggtt ccggcggcgg tgatggcctg
     3001 acggtagcgg cggtcctgca cgtccccgca ggcgtaaaca ccgggaacac gggtgtgaac
     3061 ataattctgc gttttgatgt agccgtcttc atccagatcg agttgtccgg cgaacatttt
     3121 ggcgttcgga tcgtgtccaa tgccgagaaa cataccgctt acttgcagga tgctttcttc
     3181 tcctgtgagc acattgcgcg tgcgcagacc ttttacctct ttttcctcga caccgagcac
     3241 ctcttcgacc gtggtgtttg taacgaactg gatcttcgga tgggcgatgg cgcggtcgag
     3301 catgatgcgc gaggcgcgga agtggtcgcg gcggtgaatg acgaatacct ttgtggcaaa
     3361 acgcgtgagg aagagcgctt cttccatggc agagtcgcct ccgccgatga cggcgatctc
     3421 ctttcctgaa aagaagaagc cgtcacaggt ggcgcaggag ctgacgccgt gtccgatgag
     3481 cgcctgttcg gaaggcaggc cgagccatcg ggctgacgca ccgctggcaa taatgagggt
     3541 gcgtgtgtgg atggtctcgc tgccgaggtt gagcgcaaag gggcgtttcg agaggtcggc
     3601 ggagacgagg tgggccatgc ggagctcagc gccgaagcgg gacgcctgct gcttcatatt
     3661 ttcaatgagc tgcggcccct gaatgccttc cgggaaccct ggaaaattct cgaccagagt
     3721 cgtgatggag agttggcctc caggctcgtg gccttcgagc acgagcggct tgaggttggc
     3781 gcgggcggtg tagatggctg cggtcagccc ggagcagccg gaaccaagga tgacggtgtc
     3841 gcgaatattg tcagacatag tgggaaccaa acttcgattg tatccgttca ggacgggtag
     3901 gtgagagatg ctgccagatg gcagaaagat gcgggcggct aagataggca ttatggcgga
     3961 cctgactctt gtttatggct tgcggctttt gcccgcggat gagattgcag aagtgggcga
     4021 ggccccgatt caactgaaaa acggcaacac ggcgcgggtg acgatgcata ttctggaagg
     4081 cacgcgcgaa cagatcgagg cgcaattgcg gcagagcatt gatgcgttct ttgatttctt
     4141 tccggaaatc tgacccgaag attgtcaaga agaaagtgcg gtgcggttcc cattttggga
     4201 cggtcgttgg gtgcagtttc aggatgaaaa gggcgcgcga agacgcaatc gttttccgct
     4261 aaagttgcgg tcttttcatg gcggaatgga agcatgttcc gctgtttacg caaatgggtt
     4321 gtgctctttt cactgctgct ggtttcacag gccggcctgt gtggcggacc acgctgggtg
     4381 gccgggccgg cctatttcga cccggcggtc aaagggcagg tcgtccattg gccgaatggg
     4441 acagtttcgt attatctcga ccagggtgct ttaagcccat acgtagattt ggggcaggtg
     4501 gcgaacatga ttgccgcggc tgcggcggtc tggagccagg tccccacagc ggcggtgagc
     4561 attacgcagg gcggaacgct ggatgaggat gtaaacggca gcaacgtttc agcaaacccg
     4621 gatgggagcg tgaatgtgcc gtccgacgtc cagccttcgg caaccaataa gccggttgcg
     4681 attgttttcg atgcggacgg gtcggtcatc aacgcgtttc tgggagcagg agcaagcgat
     4741 ccgtcggact gccgtgacaa tggcgtggtc acatttgtgg acaacatcgg cacggatgcg
     4801 gccatccggc acgcgctgat gattctgaat gggctgtgcg cgtggaacag cagtcagctc
     4861 acagtgatgc aatatcaggt gatgcgaggc tttgggcgtg tgctagggct ggactggtcg
     4921 cagacgaatg aagaaaactt tgccactcat accatcacct cagacgacct tgtgggatgg
     4981 ccggtcatgc atccggttga gtatctctgc cagttttccg ggacattctg ttcgccagac
     5041 ctgacggccc tgcgctatga cgatatcgca gcgataaacc ggctgtatcc ggtcacggcg
     5101 tcgaacgtca gcgcctttcc gggaaagttg ctgacggcgg ctgcgaccgt ttcagtccag
     5161 ggcaccattc atttccgaaa cgggcagggc atgcagggcg taaatgtggt cctgcgcccg
     5221 atcaacgccg gtacgggact gcctgatgtc cgttacaccg tcaccgcggt cagcggaagg
     5281 ttttttcagg gcaatgcgcc aaatcccgtg aatggcgcaa gcgctgcaag caattacggc
     5341 agcgacgatc cttcgctgga aggatggttt gatctgagcg gggtgcccct cccgccgggc
     5401 acgaatattg cggactacca gctcagcttt gagccgatca atccactgta tacggcgaat
     5461 tactccgttg gtccatacac gacaggacaa gttacacctt caggcacgat gcccagcatc
     5521 atgctctatg gcctgacagc agggaagaac tttacacagg atgtggtcat tcaggattcg
     5581 gcagatgagg aatataccgg ccatgatggg acggaaacgg ccccggcgcg gattccggca
     5641 gggggcgaat ggacggggcg catcacagga tacggccaca cggcatggtt tgactggcag
     5701 gtgcgcccca atcgcgagct tacggtagaa gcggtggcgt tggatgaaag cggcgctccc
     5761 actctgaata aggcgcaggt tgtgcttgga gcatggaatg gctccgatgc gccgggcgga
     5821 ccgccagtca gtgcgaccac gcaggcattt aacggagcgc agaccggact gacaacgctt
     5881 tctgtggcaa cggttgccgc cgggcacctg cggctgggaa tcgccgatgc acgcggcgat
     5941 gggcgtcctg actatctcta ccatgcgcgt ctgctctatg ccgattccgt gatgcccacg
     6001 cgaatctctt taagcggagg accgatcatc attcgcggca ccggcttccg cccgaatgcc
     6061 atcgtgacgg tcaatggcgt ccgtgccgcg gtaacaggcg taacgtcaac agagattacg
     6121 gccgtcgctc ctcctgccaa cggaaaaacg ggagtggtac tgcttacggt gagtgatccg
     6181 cagacgctgg cagttacggc catccaggat ggcctgagct atgacgcgct gggcggcgac
     6241 gctctgggga ttgtgactgc gccgtatggg cagacgaatt taggtgtatg gacgccgttt
     6301 acggtcaagg cactcgcggc agatgggcag accccggcgg cgggcgtaat tgttactttt
     6361 tcagttacaa atgggacagc ggctctgggt tgcggacagg cagcatgctc cgtgctttcc
     6421 ggtggcgatg gtctggcaac catcaaggtt gctgcaaact cgacctcgcc ggcgcaggtg
     6481 actgcatcgc tcagcgatgg cgcaagcatc atgacggaat ttacagggag cggcacgccg
     6541 atgatcgccg cgcttacacc gtatctttac gtcgctgcgg gagcttcatt tagttgggac
     6601 ccgcaggcgc agactttgct caacggtgtt tccatgcctg ggcagccagt gctttggtcg
     6661 gcttccgcag gagcaacggc gggcacatct tccaccctga gcgattccag cggaacagcg
     6721 accggacaaa tcaacatcag ccccttggca gcaggggcgg acgttcctgt ctatgcctgt
     6781 ctgtcaggca atgtgcagtg cgctccgttt cacatttaca gcgtgcatcc ggagattgcg
     6841 caactgtccg cagtgagcgg agtggggcag acgatcagcg ccagtgaaac gcctgtgccg
     6901 gtcgtgctgc gcgtgacaga cgcggttggg catccgatgg ctgcgggcac ggtcaccttc
     6961 tacgaaacgc tgaagcagtg gacgccggat tgccctccgc agggacgctg cccatctgct
     7021 cctgtgatcg ctacgcagat tgttccagca gtatcgggag cggacggatt gattacacta
     7081 aatccattga cgggaaatgg cgtcccgacc cggctgtatg tcacagccgt aaccgggaat
     7141 tcggcatcac tgaatttcga gattgagcag catccataac aatttcctga atttgtgccg
     7201 gcggcagcac ttcccgcatc tttcttatag atacttttag acacttattt tgggggcttg
     7261 catggctgcg gaacaaattc cagcgattcg ggaaggacgg cgtcccagag tgctgattct
     7321 tggtggagga tttgccggac tgcatgcggc caagggcctt gcaaagcttc cggtagacat
     7381 tacgctggta gaccgccgca accatcacac cttccagccg ctgctctatc aggttgcgct
     7441 ggccgtgctt tcgccggcgg acatcgccac accgattcgc ggcattctgc gcaggcacaa
     7501 gaatgtcgaa gtcatcatgg atgaagccat ccagattgac aaagagcgcc acatcgtcag
     7561 gatgaagagc ggagcggaga tggaatacga ctatctcgtt gtggccaccg gttcaacgca
     7621 ttcgtatttc gggcacgatg agtgggcgca ggttgctccc ggcctgaaga ccgtggaaga
     7681 tgcgactgag atccgccgcc gcgtgctgct cgcctttgag ctggccgagc ggcaaatgct
     7741 ggagacaggc tcgcatcctc cattgaattt tgtcgtcatc ggtggcggcc ctacgggtgt
     7801 ggaactggcc ggggccatct cagacattgc gaagctttat atgcggcatg acttccgcca
     7861 tattgatccg tcaaaggcgc gagtgatttt gctggaaggt gggccgcgca ttctgactgc
     7921 atatcctgaa gacctctcga aaaaagcagt cgagcagttg aatgatcttg gggtcgaagt
     7981 gcatacagga gcacaggtga cagatgtgca gcccggatat gtagtcgtcg gcggacaaag
     8041 gctggatgcg gttgtaacac tttgggccgc gggcgtgcag gcttctccgc tgggcaagct
     8101 gctgggcgtg gagacggacc gccgcggctg cgtgatggtc aatgccacgt tgaatccatc
     8161 cgggcatccg gaaatttttg tctgcggaga ccttgcgcat ttcgagcagg atggcaaaca
     8221 ggtccccgga gtggcgcaac ctgccatgca gatgggtgat cacgtggcgc gcatgatcga
     8281 gcaggacctg aaaggcacag cgcgcagcgg ttttcattac ttcgacaaag gcgacatggc
     8341 caccattggg cgcatggcgg ctgtcgcaaa aattgagtgg ccctttaaag cgcattggag
     8401 cggctttatg gcctggatta cctggctgac aatccacatc ttcttcctga ttggcttccg
     8461 taaccgcatc tttgtgttct gggagtgggc ctggacatat ttcaccttca cgcatggcgc
     8521 acgccttatt accggaagcc aggtattgcc ggggtggacc gagcagcagg gcgtggaaca
     8581 agaagaagtg agcaagcctc tcgaccctac ctcacccgga ttcagaaatc atcatggaga
     8641 agtggccgcg gcaggcgaaa aacggtagca gaggacacgc ggtttcgtgt tgcgaatgca
     8701 gactgtcaag ggctaatttg ctttcgatgc atttcgcgct aggacgcggt ctaccatgct
     8761 ttccacatcg tgcaggaatc cggtgcgctc ctcagccggt tcttccggtg gatggagcgc
     8821 aagcgcgaga tcattccagc gcaacagggc cttcataggc gcatgagcaa attctctgac
     8881 tggttcaggc gagaggaaga aaccaatcca gacaaccaga cagaggatgg tgaccgagta
     8941 gcagatgtaa accgcaacgg aagctgcatg ggccaaactt gccgagaagc ccgcaatgtc
     9001 tgccagggca agcgccatca gtccaatgca gatgcagaaa atgcggcttt tggcggaaaa
     9061 gccaagtgcc cgggctgcca caaccagaaa agcaaccagg caaagttcca gggcacagat
     9121 gcctgtcgcg atctgaagaa gaacaaactc tatgtggctg ccgtgcggat gcagcaacga
     9181 aggcaggacc acggcacagg aaagcagcag ggaaacaaga gcgacccagc gaaaaacaat
     9241 gcctcccata tgaagcagtc cgggaagcgg agcaagaatg tggcggaaga tttccttgaa
     9301 tgcgaagagg agcaccacaa atccagccag aaaccccgtc cagctcgtat agaaagcgat
     9361 cggcggcagc acactggctt gtccaggctc gggatgccgt aagaagagga cagtcagcac
     9421 aaacgccgtt aatgcccgga tgcccaggaa gattccgaaa aataacgaaa gcaagtgtcc
     9481 tctggcccgt aagtatccaa tcagcacgag tagccacagc cagggctcca tgctgcgggc
     9541 cagcgaacta aacggactaa cggccaaaaa tgtcatcctg cggaaccagc cccaatggga
     9601 aaaataaaaa agagtacgac ttcttaggtt agactgcctt gactgggcaa gcaaggcaga
     9661 aatacagggg cttcatccgt aagtaatgtt cataatggtt accctgcagg gcgtgcagcg
     9721 caatccacag ttccagacgg tccaaagtgc tataattgaa taagaaaatc aaggcagaaa
     9781 cctcttcgct ttcaatcggt tatctaaaat caggccgttc tggtccgagg cgatggaagg
     9841 gctgctttac cggagtttca tgtccaccaa agagcttgcc gtttcaacaa cttccctcga
     9901 cgagatggag cattcctcgc ctgaaaaaaa tggcaggaaa tctacaaatg acctcgaaaa
     9961 gaactacacc ggcgaaaaca ttaaggtcct tgaggggctg gaggcggtcc gtctgcgccc
    10021 tgccatgtac attggctcaa cgggcgagat gggtctgcac catcttgtct acgaggtagt
    10081 ggacaactcc gtagacgaag cgcttgccgg atatgccaaa aagattgacg ttgttattca
    10141 tgtagataac tccatcaccg ttattgacga tggccgcggt attccggtgg acgagatgac
    10201 actgcccggc ggcgaaaagc tgcctgccgt ccaggtggtg ctgaccaagc tgcatgccgg
    10261 cggtaaattc gattcctcca cgtataaagt ttcaggcggt ctgcatggcg tgggtgtatc
    10321 gtgcgtcaat gcgctgagcg aagagtttga tgtcgaaatc tggcgcgatg gccacaccta
    10381 cgaacaggac tacgagcgcg gcattccggt ctcaaaactg cgccagacgg gcacctcaag
    10441 aaagcgcggg accaaggtcc atttcctgcc cgacaagacc atttttacca ctacggaata
    10501 caactacgac acattggcac agcgtctgcg tgagcttgcc ttcctgaaca aggggcttga
    10561 aattacgctc tcggacgagc gcacgaccga tcctaaaaca ggcgaggcaa agcgcgccga
    10621 gttcaaatac gtgggcggta tcgctgagtt tgtaaagcac ctcaacaaag gcaagcaggt
    10681 gctacatgac aagccgattg tgatggaagc agtgcgcgat ggcgtggaca tggaaattgc
    10741 tctccagtac aacgacgggt actcggaaac ggtcttttcg tttgccaaca acatcaacac
    10801 ggtggacggc ggcacgcatc tttccggctt ccgcacggcg ctgacgcgca ccatcaacgc
    10861 catcggccag caaatggggc tcttcaagga cgtaaaggag aacctctccg gcgacgatgt
    10921 gcgtgaaggg ctggtggcag tcatcagcgt aaagctgccg cagccgcagt ttgaaggcca
    10981 gaccaaaggc aagctgaatt cggatattgc agggaccgtg caggcgctcg tgaatgaaaa
    11041 gctgggcatg ttcctggagc agaaccctcc ggttgcccgc cgcatcatca acaaggcgat
    11101 tgaggccgcc cgcgcccgcg aggcagcacg caaggcccgc gacctgaccc ggcgtaaagg
    11161 cgcgctcgat tccggcggcc ttcccggcaa gctggccgat tgcagcgaac gccagcccga
    11221 ccgctgcgag ctatatctgg tcgagggaga gtcggcaggc ggaactgcaa agcagggacg
    11281 cgaccgccgt ttccaggcga tccttccact gaaaggtaaa atcctcaacg tagaaaaggc
    11341 ccgctatgac aagatgctgg gccatgagga gatccgcgcc atgattacag cgctgggcac
    11401 gggcatcggc aaagacgact tcgacgcctc gaagctgcgc tatggcaaga tcattcttat
    11461 gaccgacgcg gatgtggacg gctcccacat ccgcaccctg ctgcttacgt tctttttccg
    11521 ccatatgaat gagctgatta agcgcggtca cgtctatatt gcgcagccgc cgctgtatcg
    11581 catcaagaag ggcaagttcg agcagtacat caaggacgac cgcgaatttg tccgcgtcat
    11641 ggtgaagcgc gcctcggacg gaatgattgt gcgtcatggt gagaacgcga ctaagctcga
    11701 aggcgcggcg ttgacgaaat tcatcgggca gttaaatgag tatcttggct tcttcgataa
    11761 ggtgaataag cgcatccgca atgaagaagt gacagggttg tttgctaagc tgtttgcgca
    11821 tgagggcaag gagcctgtaa aacgcgcaga ctttgagggc gatgcgaaga cggtcccagc
    11881 caagcttact gcgctttatg acgagctgaa gaagattgag aagaggtatc agttcaagtc
    11941 gcttgacaag cccgtctatg acgaggagca ccagacatgg tcgctcctct ttcatgacgc
    12001 gcagggtgct gagcgcaaaa ttgactgggt cttcgcatct gctccggaga cgcgccagat
    12061 gcttgccaag tatgcgcaga tcaaggacgc aatcgagcct cccttcctga tcgaatatgc
    12121 gggcaaggct gccgtggctg aacctgatcc ggaagagaac gagggcgaaa ccacaggcga
    12181 ggccgaggcc ggcaaaccgg cgccaaagcg cgctggacgc gcctcgcacg atccggtgga
    12241 gaagcaatcg ccgcgcgagc tgttcgagta tgtgattgaa cagggccgcc gcgattatca
    12301 ggtacagcgg tacaaaggtc tgggcgaaat gacctcgtcc cagctatggg aaaccacaat
    12361 ggatcccgag cgtcgcacct tgctcaaggt gaagctcgaa gatattgcgg agacggaaac
    12421 catcttcacc acgctgatgg gcgaagacgt ggaagcgcgc cgcaagttca tcgaagacaa
    12481 cgcgctcgat gtgaagaacc tggacatctg agtggtttgt aaatggaagc ataaagtgaa
    12541 atatggctgg tttcgagata tttcattcac agttgagtaa cctttaacat ctctacatgg
    12601 cgcaggggta ctttcagcct ggaacgggtg gccggttttt tatactggaa ttgctggcct
    12661 ccattctgct ggaattgaca ctcttccacc tgccgccata gatgctctct cgctttcatg
    12721 acgatccaac ttctccacac ccccgacgac tgcggcggga tatctactcc gccagctgat
    12781 cggcctcgtt atttctgaac cagttgggat gttagtcttc ggccagaact gagcctgtca
    12841 aggcggaggg ttggctgatg aagaagcgct tcagtgtgga acagatcata ggcgtgttga
    12901 agcaggcgca ggtaggagtc ccggttgcag aagtgatccg gaaggctgcg atcagcgagc
    12961 agacgttcta ccgatggaag gcgaagtacg caggtctgga agtggacccg gtgcgccaga
    13021 tggcgcagtt gtaggaagag aaccagcggt tgaagagatt ggtggcagac ctgacgctgg
    13081 acaagacgat gttgcaggat gtgctcccac cccatttcgc tttgctcatg ggggccccgg
    13141 tgctctcaaa aaaatggtga agccttcggg gcgtggaccg atggtggagc atctggagcg
    13201 gatgtacggt gtagcggaac gcagggcatg ccgggttttg tccattccac gagcgacgta
    13261 ccgttatcgg agctgcctgg atccacgaat ggagctgcgg atgcgtatcc gggagattgc
    13321 gcaatcgaga gtgcgctatg gatatcgcaa gatccgggtt ctgttgaacc gcgcaggctg
    13381 gaacgtaggc aagtacctgg tgtaccggtt gtactgcgaa gagggcctct gcctgcagcg
    13441 cgtgcgacct gcaggcaagg gcaaggcatc acggccgcgt gctgagaagt tcaaggctac
    13501 ggctccggat caagcttgga gcatggactt cggtgcgcca tgacggcgga ggaggatgag
    13561 ttgacgtaga atgatacttc tctgcctgct ggcttctgac cgaccgttgc aatgcggtcc
    13621 gactcaggaa ggaacctgag ttgaaggggt ctgtccaagc gcagcgtgta ccgacgcagc
    13681 gcgtgaaaag ctgacagact gaactgtacc gggcgtcgcc atcatcgacg tgtcgggcta
    13741 ccacacatct tatggtgaga caacgaagca tacgccggaa gcctctgcct tgataaggct
    13801 caacgctccc gagtagacct gatggggttg ggagtcatgc gatcagaact ggtgcggcgc
    13861 gaagttcttc actggtgggt ccgcgaagcg gactcagcga cagcgaattc ctcatgggct
    13921 gttgctccga atcgccaggg gtaaagtatg ccacctacga gatgaaggtc gagagctgga
    13981 acatgggaac tcggatcaac cgcttggcaa cggagccggg ctatccacgg agactgggga
    14041 aggtggatca cgagggcgga gcctgcgtag tagttggagc acgggaaagc cgtgtacata
    14101 gcgaaggcag gcaaggaacc ggtgaagttg tggagacgga gaaaccactc atggacttgg
    14161 gtgatcaggc agacgatgtc tggcttctca tcaggcagac gatgtctggc ttctcaacgt
    14221 gcaacgcaag cttcatcaat ggagcaggaa tcatcctgaa gacagctatc gtgacctgtg
    14281 gaactggaca accgatatcc gcaatctacg atgcgcctgg cggcaaatcg ctcagaacca
    14341 agggcgacgc actgcaggag tcgacgggat gacagtagca gggatctgcg aggcaatcgg
    14401 ggtgggtctg ttcctggaac agatccgcga cgacctgaag agcggtcggt acaagccgag
    14461 cccctcacga aggaagttga ttccgaagcc gcgtcaaccg gggaagttcc gacccctcgg
    14521 tatcccgaca gtgcgtgacc gcgtggttca gtgtgcggtc aagaatatcc tggagccgat
    14581 cttcgaggcg gacttctggc atgtatcgta cgggtttcgg cccggacgcg gctgccatgg
    14641 tgccttggag cacatccgac tcagcatgtg tccgaaggtg aaggctgaag atggccgacg
    14701 ccagaagacg ccctatcagt gggtcatcga aggagacatc aagggctgtt tcgataacat
    14761 cgatcaccac cagctgatgg agcgggttcg tacccgaatc atcgatggaa aggtgacgac
    14821 gttgatcaca cagttcctaa aggccggagt tctggcagac ggctttctgc tgccaacaga
    14881 cacaggcact cctcaaggtg gtgtgatctc accgttgctc gcaaacatcg ctctcggtgt
    14941 catcgaagag cgatacgaac ggtggacaca tcatcgtcgt aagatccagg cccgccgcaa
    15001 gagcgatgct cgaacggcgg cgatgtgggc acgcatgggt gatcgtaaag ctggtcgacc
    15061 agtgttcttc ccgattcgat atgcagacga cttcgtggtt ctcgtctcag gaacttgtga
    15121 gcaggctgag caagaaaagg ctgaactcgc aagatacctg aaagacacca tgaagttgga
    15181 gctgtccctc gagaagacgc gggtctctga tctgaaagag ggttttcagt tcctcagtca
    15241 gcgcgtgcgc tacaaatggc accgtcgctt cggctacatg ccgaggatcg agataccgac
    15301 cagtaaacga gcggaccttc ggtaccgagt gaagcagttg accactcgcg caacaacgca
    15361 gtggtcgctg tctgaacttc tccagaaact caatcctatc ctgcggggat gggggaacta
    15421 ctatcgcttc tgcaccggag caagccgcca gttctcagcg ctcgactttt acgtcggcga
    15481 ccgtatctgg cgctggctga tgaagaagca caagcatctc catcgcaagc ggagacggct
    15541 agtacgagtg ccgagtctcc ttcgtccgac gcgaaaagta tggcgtgaag gaagaacaga
    15601 acagttcctt ctctccatgc tccgggtcga gcgattccgc agaggctgga tgcgcacacc
    15661 tgcatatatc tgtgttcccg gagagccgga tgcataacga aaggtgcatg tccggttcgg
    15721 agggcgagca tcagaatccg acgacagaaa tgtcgcacgg cgctgatgct cgaccctact
    15781 gtccggccag ctacagaacg gtacgaggtt ccgctcgctg acgatcgtcg agtcggccaa
    15841 agcctgaagg gcgacgatgt ggtgcgtacg ctgaatcgtg tgaagcaaga acggagtaag
    15901 cgttggtttc aggccgcatt gacatttggt tggtcgtaga gatcgtgtcc atgatgtttt
    15961 gctcgtgggc acgatagtcg gaagcggtta gtcgcaggcg gtcggagtgg cggcgaggtt
    16021 ccagggcaac agttca
//
LOCUS       DASRBV010000060         6804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_663464, whole genome shotgun sequence.
ACCESSION   DASRBV010000060 DASRBV010000000
VERSION     DASRBV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663464"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFA02_02520"
     CDS             complement(<1..470)
                     /locus_tag="VFA02_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU08750.1"
                     /translation="MKRIPFLAFILLAFAFPANAQDAIKKDAADKPAEPEMKVVLDSW
                     NEIGRKLTAIAEDFPEDKYDFKPTPAQRSFAEQLLHAAGSNYYFTDTVMGRNLPAEDP
                     KRDQYKTKADIVAFVKKSFADGAAAIQSQGEKGMMTEFVYFPHQKARVLDIAYG"
     gene            complement(515..1024)
                     /locus_tag="VFA02_02525"
     CDS             complement(515..1024)
                     /locus_tag="VFA02_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZU08751.1"
                     /translation="MTKSTVDETERMVITRVFDAPRELVWKAWTDPKYVMQWWGPSGC
                     TSPFCEMDFRVGGKFLICGRMPDGQEFWNAGEYHEIIPHEKIVSSMYFSDSKGNKIDP
                     AQIGIEHEAIDGAYDVITFEDLGNGRTKLTFIGNETMQNAIESGQAEGMNQVLDKVAV
                     VVAGLAQAK"
     gene            complement(1021..1359)
                     /locus_tag="VFA02_02530"
     CDS             complement(1021..1359)
                     /locus_tag="VFA02_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU08752.1"
                     /translation="MVVNSLDTTFAALSDPTRRAMIERLSRGPASVRGLTEPFALSQQ
                     MISKHIAYLVRAQIVIKTKRGRESICTLRPQAIKEVSDWAISYRRFWEESLDKLDVVV
                     NRMKKRGDQR"
     gene            complement(1457..1729)
                     /locus_tag="VFA02_02535"
     CDS             complement(1457..1729)
                     /locus_tag="VFA02_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HZU08753.1"
                     /translation="MSHSDREKAKLLNRVRKVRGQLDAVERALQADQDCGDILMLLAA
                     VRGGVASLMAEVLEDHIKYHLTDESKEKIAPELADDLIDLVRAYVK"
     gene            2111..4786
                     /locus_tag="VFA02_02540"
     CDS             2111..4786
                     /locus_tag="VFA02_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HZU08754.1"
                     /translation="MKTILNSALARLLFASLLLLSLPVLASDQNFQMKVNDKNYLDTR
                     GFSVFLYDSTYHPVFVDQKNAAMEMILHGQRIATNGDVRLVPTPEQWDLVATLKGRHA
                     DKANNRLTADLAFPSFDFSYTLEVAAEPGGVKVSINLDKPLPQKLAGRAGFNLEFLPS
                     IYMGKAYLVDGTNAGIFPRTPNDPMIKVLPLPDEPKKAYYLEDWDKAKGYTQPLPFAE
                     GRSITLGVDDNLARVKVTSDTTKLMLFDGRDRAQNGWFVLRSLIPSGKTTGAIVWHIH
                     PDVIPNWTRPPVIAHSQVGYVPGFSKVAVLELDPNYNGPKTARVLRLMEDGSYKQVFE
                     GAITPSTPWLRYVYSKFDFTPVNDPGLYVIEYADQRTAPFPISKNAYANIWQDSLDHH
                     LAEQMDHVSVREGYHMWHGASHLDDGRMAPVVGEQFDGWYQSTATDGKYKGGDHIPGM
                     NVGGWYDAGDFDLEEPAQLSVIQSLALAYREFNLKYDELTVDEAARSVEMHRPDGVPD
                     TVEQVRHGALYILAQFHNIGHAIRGTHEPDLRQYTQIGDGASKTDGRIYDPKLGPNEV
                     KGDYSGKPDDRWIFTTNNPFFQWNAIAALAAAADVLKGWDDTLAKDCLDTAIKAWNDE
                     KAHPTQFQGRVPGSGVLAASQGVVSHNRQGRPGTSTSGGTNAPPAPPAGFNVGLDWAA
                     ALELTIATHGAEPYRSRLKELFPQMITPQQMGIRGWTAVRALPYLDPGAKDQMREAVK
                     TYIANLDKQLDATPFGVPPSLGTWGGSGAVVDMAIRMYFLHKTFPDLVGPEYTLRAVN
                     YILGTHPVSSTSYVAGVGTVSKTKTYSNNRADNSYIPGAVIPGYVIIKPDFPECIDDF
                     GYLWFEDEAVVAGSASWVVAGNAADAITKESKQAQ"
     gene            complement(4818..5507)
                     /locus_tag="VFA02_02545"
     CDS             complement(4818..5507)
                     /locus_tag="VFA02_02545"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZU08755.1"
                     /translation="MKVRREQNLARWGKHACRAGFVSLCAFALAITPSAVFAGQHGGG
                     HPHFSPPPRMAQPRPSAPTFHPVQPQPRVMNPGANQARPGHLPEWLNKHQNLSPQQQE
                     DALRHEPGFKSLPPQQQQRLLQRLHTLDSKPPEVRQRLAQRNEMYEHLTPEQKANVRG
                     ALQAFQQMPPDRQTSMRRAFQDLRAVPPEQRQSVLNSARFQQEFSPRERTILGNMLTI
                     EPYQPRAGAPQ"
     gene            complement(5619..6122)
                     /locus_tag="VFA02_02550"
     CDS             complement(5619..6122)
                     /locus_tag="VFA02_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08756.1"
                     /translation="MICRKVKANLADLLLDSESVSAEVRNHLKDCAACRVELASLEAT
                     MNLMDEWHAPEPSPYFDSRMSALLREEQRAEPAGFFERVKARVLYGSNMHLRPVAAGA
                     LALLLIVGGGTYAGLMTSSHSTSNARVSAVVEDLKSLDENAQMLQQLNALDEEAQGGN
                     SASPDNL"
     gene            complement(6137..>6804)
                     /locus_tag="VFA02_02555"
     CDS             complement(6137..>6804)
                     /locus_tag="VFA02_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU08757.1"
                     /translation="KSSMATIALDRPVFNDVRNEVVRRERIADWSALSDAEIMLRVSQ
                     GDDAGFNYLIEKYRKPIIHFMFRMVHNQAIAEELAQEVFLRVYRSRETYRAEARFTTW
                     LYRIATNLGVNYARDTKHERAAQNVYLDQPDPETGTTPDLADTTPSVEHDLVREERMR
                     AIRQHVMALPERQRMAVLMHKYQGMDYKQIGEVLKLSESATKSLLFRAYQTLREKLKE
                     FVA"
BASE COUNT         1517 a         1942 c         1819 g         1526 t
ORIGIN      
        1 atgccgtatg cgatgtcgag aacgcgcgct ttctgatgag ggaagtaaac aaactccgtc
       61 atcatgcctt tttcaccttg cgactgaatg gcggcggctc cgtcggcgaa ggatttcttt
      121 acgaatgcga cgatatccgc ctttgttttg tactggtcgc gctttggatc ttccgcggga
      181 agattccgcc ccatcaccgt atcagtgaaa tagtagtttg aacccgccgc gtgcagcaac
      241 tgctcggcga agctccgctg cgcaggcgtg ggcttgaagt catatttgtc ctcgggaaaa
      301 tcttcggcaa tcgccgtcag cttgcggcca atctcattcc aggaatcgag cacgactttc
      361 atttccggtt cggcaggctt gtcggctgca tctttcttga tcgcgtcctg ggcatttgcg
      421 ggaaatgcaa atgccagcag gatgaaagcg agaaacggaa ttcttttcat ttctttctcc
      481 accattgagt ttcacgaatc aaagcttcag cttcttattt agcctgcgcc agtcccgcaa
      541 caactacagc aactttatcg agtacctgat tcatgccctc cgcctgaccg ctctcgattg
      601 cgttctgcat ggtctcattc ccaatgaagg taagtttcgt ccggccgttt ccaagatcct
      661 caaacgtgat cacgtcgtac gcgccgtcta tggcctcatg ttctattcca atttgcgcgg
      721 gatcaatctt gtttcccttc gagtcggaaa agtacatgga ggaaacaatc ttctcgtgtg
      781 gaataatctc gtggtattca ccggcattcc agaactcctg gccgtccggc attctcccgc
      841 aaatgagaaa cttccctccc acgcgaaaat ccatctcgca aaaaggcgaa gtacagccac
      901 tgggccccca ccactgcatc acatatttcg ggtccgtcca cgccttccag accagttcgc
      961 gcggggcatc aaaaactcta gtgataacca ttcgctccgt ctcatctacc gtgctttttg
     1021 tcatctctga tctcctcttt ttttcattcg attcacaact acgtccagct tgtccaaact
     1081 ctcttcccag aaccggcgat agctgattgc ccagtcgctg acctccttga tcgcctgtgg
     1141 cctgagcgtg cagatactct ctcgtccacg cttcgtcttg atcacaatct gcgcccgcac
     1201 caggtaggca atatgtttcg aaatcatctg ctgcgagagt gcaaacggtt ccgtcaagcc
     1261 acgcacagat gcaggcccgc gagagagtcg ttcgatcatc gcccgccggg ttggatcaga
     1321 caaagccgca aaggttgtat ccagagaatt cacaaccata tggtagtgga tttcagttga
     1381 atgtcaattg ttatttccgt cccggcttca atcgccaatt tcccagcggg cctctttacg
     1441 aggtctgggc aggactttat ttcacatagg cgcggaccag gtctatcaga tcatccgcta
     1501 attcgggcgc aatcttctct ttcgactcgt ctgtgaggtg gtacttgatg tgatcttcca
     1561 ggacctctgc catcagtgag gcgacgccac cgcggacggc ggccagcagc atcagaatgt
     1621 ctccgcaatc ctggtcggcc tgaagggcgc gttctactgc gtccagttgt ccacgtactt
     1681 ttctcacccg gttcagcaac ttcgcttttt cgcggtctga atgcgacatt tctctccttt
     1741 gaggaaaacc ctgtggccgt atggtcccca acccagaggg ggtatagatt actcctgcat
     1801 gaaggatata tggacagaaa tcctgcgcaa atggagcccg aatcagcatt ttcactcatt
     1861 gcctgcatgg atcaaagccc ggaacttcgg gagtaatcac aaaaataaat tgatttacct
     1921 tgacagttgt cgaaggcagg cgctaaaaag aacacggccc gaaaacaatc gccgaagcat
     1981 tcaggaagag tcgtcctcag caccataaag tgcaggctgt tcctctctgg gtgccaatgg
     2041 cggacgatca ccaaacactg aacttcctga cgtcagtgcc aggacctggc aagcccgcat
     2101 taggacaagc atgaagacga ttctgaattc tgcacttgct cgcttattgt tcgcatcttt
     2161 gctgcttctg tcgctccccg tcctggcctc agaccagaac ttccagatga aggtgaacga
     2221 caagaactac ctcgatactc gggggttcag cgtatttctc tacgacagca cataccatcc
     2281 ggtttttgta gaccagaaga atgcagccat ggagatgatt ctccacggcc agcgcatcgc
     2341 cacgaacggc gatgtgcgcc tggtgccaac ccccgagcag tgggacctgg tggcgaccct
     2401 caaaggacgc catgccgata aggccaacaa ccggctaacg gccgacctcg ctttcccgag
     2461 cttcgatttc agctataccc tggaggtcgc agccgaaccc ggtggggtga aggtcagtat
     2521 caatcttgac aagccgctgc cgcagaagct tgctggcaga gcgggcttta acctggagtt
     2581 tctgccttcg atctacatgg gcaaggctta cctggtggac ggaaccaacg ccggcatctt
     2641 tccgcgcacg ccaaacgatc cgatgatcaa agttttgcct ctgcccgatg aacccaaaaa
     2701 ggcgtactac ctggaagact gggacaaggc caagggatac acgcagccgt tgccgtttgc
     2761 cgagggccgg agtatcacgc tgggagtgga cgacaacctg gcccgggtca aggttacgtc
     2821 agatacaacc aagctgatgc tgttcgatgg acgcgaccgg gcacagaacg gctggtttgt
     2881 tttgcgctcg ctgattcctt ctggcaagac tacgggtgcc attgtctggc acattcatcc
     2941 agacgtgatt ccgaactgga cgcggccgcc agtcatcgcg cacagccagg ttgggtatgt
     3001 gccgggtttc tccaaagtgg cggtactcga actcgaccca aactataacg gtccaaagac
     3061 ggccagagtt ctgcgcctga tggaggacgg atcgtacaag caggtgttcg agggggcgat
     3121 cacgccgtca acgccatggc tgagatatgt ctattcaaaa ttcgacttta ctccagttaa
     3181 tgatccggga ctgtatgtca tcgagtatgc agaccagcgt actgccccct ttccaatctc
     3241 gaagaacgct tacgcgaaca tctggcagga cagcctggac caccaccttg cagagcagat
     3301 ggaccatgtt tccgttcgcg aaggctacca catgtggcat ggcgcttcgc acctggatga
     3361 tggccgaatg gcgccggtgg tgggcgagca attcgatggc tggtatcagt ccacagcaac
     3421 ggacggcaag tataaaggcg gcgaccacat accagggatg aacgtgggcg gctggtatga
     3481 tgcaggcgac ttcgacctgg aggagcctgc gcaactcagc gtgattcaga gcctcgctct
     3541 ggcataccgc gaattcaatc tcaaatacga cgagcttacg gtggacgaag cggcgcgttc
     3601 ggttgagatg caccggcccg acggcgtccc cgatacagtt gaacaagtca ggcatggagc
     3661 gctgtatatt ctggcccaat tccacaacat tggtcatgcc atccgcggca cgcatgaacc
     3721 ggacctgcgg cagtacaccc agattggcga cggagcatcc aaaaccgacg gccgcattta
     3781 tgacccgaag cttggtccga atgaagtgaa aggcgactac tccggcaaac cggatgaccg
     3841 ctggattttt acgacaaaca acccattttt ccagtggaat gccatcgcag ctcttgctgc
     3901 cgcggcagat gtcctgaaag gctgggacga tacgcttgcg aaagactgcc tcgatacagc
     3961 aatcaaagca tggaacgatg aaaaggccca cccaacgcag tttcaggggc gtgttcccgg
     4021 aagtggcgtg ctggctgcgt ctcagggggt ggtgtctcat aaccggcagg gcaggccggg
     4081 cacttcaact tctggcggaa cgaacgcacc gccagctcct ccggctggat tcaatgtcgg
     4141 actcgactgg gccgcggcac tggaattgac cattgctacg catggcgctg aaccttacag
     4201 gtcccgcctg aaggagttgt ttcctcagat gatcacgccg cagcagatgg gcatacgcgg
     4261 ctggacggcg gtccgggcgc tgccttatct ggacccaggt gcaaaagatc aaatgcggga
     4321 agcagtgaag acatacatag cgaatctgga caagcagttg gatgcaaccc ccttcggcgt
     4381 gcccccaagt ctgggcacat ggggaggatc gggtgccgtg gtggacatgg ccatccgaat
     4441 gtattttctg cacaagacct ttccggatct cgtaggtcct gaatatacgc ttcgcgccgt
     4501 caattacatt ctgggtacgc acccggtatc cagcacttcg tatgtggccg gcgtcggcac
     4561 ggtctcgaag acaaagacat acagcaacaa ccgcgccgac aattcctaca taccgggcgc
     4621 cgtgattccg ggatacgtca tcatcaagcc ggattttccg gagtgcatag acgacttcgg
     4681 atacctctgg ttcgaagatg aagccgtggt ggccggatcg gcaagctggg ttgtggccgg
     4741 caatgctgcc gacgcgatca cgaaagaatc gaaacaggcc cagtagggaa cgcctgattt
     4801 ggtaaaaggg gcggaagcta ctggggagcg cccgctctgg gctgataagg ctcaattgtt
     4861 agcatattgc ccaggatcgt gcgttcccgc ggagaaaact cctgctgaaa gcgcgcagaa
     4921 ttcagcacag actggcgctg ctctggcgga acagcgcgta aatcttgaaa ggcccttcgc
     4981 atagaagtct ggcggtcggg cggcatctgc tgaaatgcct gcaatgctcc gcgcacattg
     5041 gctttctgct ctggagtcag gtgctcatac atttcgttcc gctgcgcaag acgctgacgt
     5101 acctctggcg gtttgctgtc caaggtatgc aaacgctgca acaggcgctg ctgttgctgt
     5161 ggcggaaggc ttttgaatcc aggctcgtga cgcaaagcgt cttcctgctg ttgcggactc
     5221 aggttctggt gtttattcaa ccactccgga agatggcccg gacgcgcctg gttcgccccg
     5281 ggattcatga cccgtggttg cggctgtact ggatggaacg ttggagcaga tggccgtggt
     5341 tgcgccatgc ggggcggcgg agaaaaatgt ggatgccctc cgccatgctg ccctgcaaaa
     5401 acagcgctgg gggtaatcgc taaagcaaat gcgcagagag acacaaatcc ggcccggcaa
     5461 gcatgcttac cccatctggc caaattctgc tctctgcgga ctttcatgag tgtaacttcc
     5521 agcgcatgat ttcaaaatgt ttatacaccg ccacgcccac tgtgcgcgaa cgttgcatct
     5581 gctttctgag cgcggcgcca taggatatcg tttccggcct agaggttatc aggcgaagca
     5641 ctgtttccgc cctgtgcttc ttcatcaagc gcattcagct gttgcaacat ctgcgcattc
     5701 tcgtccaaag acttcaagtc ttcaacaaca gccgagacac gggcattcga agtggaatgc
     5761 gaagacgtca tcaaaccggc atacgtaccg ccgccaacaa tcagcaaaag ggccagtgcc
     5821 ccggcagcca ctggacgcag gtgcatatta ctgccataca gaacgcgcgc cttcacgcgc
     5881 tcaaagaagc cggcaggctc cgcgcgctgt tcctcccgca gaagcgccga catccgggaa
     5941 tcaaaatacg gcgaaggctc cggagcatgc cattcgtcca tcagattcat cgttgcttcc
     6001 agcgatgcaa gttctacgcg gcacgcggcg caatctttca gatgattgcg cacctcagct
     6061 gaaaccgact cagaatccag gagcagatca gcaagattcg cttttacttt acggcagatc
     6121 ataaaatttc cccgatttat gcaacaaatt cctttagctt ctcgcgcagc gtctggtacg
     6181 cccggaaaag cagtgacttg gtggcggact cactcagctt taaaacttcc ccgatttgct
     6241 tgtagtccat gccctgatat ttatgcatca gcacggccat gcgctggcgt tccggcaggg
     6301 ccatcacatg ctgacggatg gcgcgcatac gctcttctct gaccagatca tgctctaccg
     6361 acggcgttgt atcggccagg tcgggagtcg ttccggtttc cgggtctggc tggtcgaggt
     6421 aaacattctg cgcagcccgc tcgtgctttg tgtcccgcgc gtagtttact cccagattcg
     6481 tcgcaatgcg atagagccag gttgtgaacc gtgcctcggc gcggtacgtc tcgcgcgagc
     6541 ggtagactcg caggaagacc tcctgcgcta gctcctcggc aatcgcctga ttgtggacca
     6601 tgcggaacat gaagtgaatg ataggcttac ggtatttttc aatgagataa ttaaagcctg
     6661 catcatcccc ctggctgacc cgcagcatga tctctgcatc gctcagggcg ctccagtccg
     6721 cgatcctctc gcgccggaca acctcgttcc ggacatcatt gaataccggt cggtctaggg
     6781 caatggtcgc catactactc ttag
//
LOCUS       DASRBV010000061         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_207885, whole genome shotgun sequence.
ACCESSION   DASRBV010000061 DASRBV010000000
VERSION     DASRBV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207885"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFA02_02560"
     CDS             <1..243
                     /locus_tag="VFA02_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HZU08758.1"
                     /translation="AQVSRVLYQLPCYCRCDKALGHTSLHSCFEGTHGAICSTCAKEG
                     AYAYKMTKMGKTPKQIREGIEHNEYQTIDLNQLGGM"
     gene            268..918
                     /gene="tmk"
                     /locus_tag="VFA02_02565"
     CDS             268..918
                     /gene="tmk"
                     /locus_tag="VFA02_02565"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZU08759.1"
                     /translation="MACGFFLTFEGLDGSGKTTQIRKLTARLESQGHKVIVTRQPGGT
                     RIGDRIRQLLLDSRTENLAPLAELGLMFSDRAQAIAEVIRPALDAGHIVVCDRYTDST
                     EAYQGGGRQLGSEIVLKLHNAICEGLQPDLTILLLPDFDRSLHRARRRNERNAKSGAD
                     ENRFEREAEDFYRRVHDKYREIAQRDAQRVVAIEGDAGIEEIHEQIAKIVEERLKR"
     gene            1087..3510
                     /gene="lon"
                     /locus_tag="VFA02_02570"
     CDS             1087..3510
                     /gene="lon"
                     /locus_tag="VFA02_02570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZU08760.1"
                     /translation="MVTSKDVRDKNELRKLPMMPIRDMVIFPYMMTPFVVGRETSVRA
                     LEEALTGDRKIFLATQHDARVDEPKASDIYSVGTIGNIVQSVKMPDGNIKVLVEGLER
                     AKAVEMNDADGFFVATVRVPKIDIERASSIEQLTQRVTSLFEQYIKLQQSLNYETMIA
                     AVRTDEPGKLADTIAANLQLEIPEKQELLEIFDPAERLLRLADVLDIEIEKLNMERSI
                     QSRVKRQMERAQKEYYLNEKIKAIQKELGRSEKSEFDDLKKKIESAGMPRPVFDKAIQ
                     ELKKLEAMPPMSAESTVSRNYLDWLLAVPWKKKSKETRSIEKAEKVLNEDHYGLEKIK
                     ERILEFLAVRQLVKNPKGSILCFVGPPGVGKTSLGMSIAKATGRKFVRMSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQSMKKAGTKNPVFMLDEVDKMASDFRGDPASALLEVLDPE
                     QNHSFQDHYLDVEYDLSEVLFVATANVLHTIPAPLQDRMEILRLHGYTEAEKLEIARQ
                     YLIKKQRENTGLTEKNIVFTDEALIAMIRSYTREAGVRNLEREIGNICRKVARRVVKA
                     GAKHKEEITPDNLADFLGVARFRDSQVHEKSEVGLVTGLAWTEVGGSILPAEVQILDG
                     KGKLTLTGQLGDVMQESAQAALSYVRSRALHLGLSRDFYRNVDIHIHVPEGAIPKDGP
                     SAGITMATALASALTKIPVRRDIAMTGEITLRGKVLPIGGLKEKLLAAHRAGIFEAIL
                     PRDNEKDLAELPDNLKSAMKLHFVDSMDEVLQLALEGPLPELSEEPAAVLPSVPPPAV
                     TAPEQRPHQ"
     gene            complement(3624..>4291)
                     /locus_tag="VFA02_02575"
     CDS             complement(3624..>4291)
                     /locus_tag="VFA02_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU08761.1"
                     /translation="KFTLGQRIALAIVPRLTAVVLAVIGATLRFEVIAEPGATPATPP
                     ATGIFCFWHRCTLPCAWYFRKFRCSILISRSFDGELIARTLALLGFDSVRGSSSRGGA
                     AGLIALEQVLEQGLPVVFTADGPRGPIYQTKMGPVKLAQMTQQEIGSFYLLPERAWTM
                     RSWDHFLVPRPFSRVAVSWARPVAPPAEDADSAALEAKRQELNDALERARQNAERYFL
                     AQK"
BASE COUNT         1045 a         1180 c         1204 g          862 t
ORIGIN      
        1 gcccaggtgt cccgcgttct ctaccagctt ccctgctact gccgctgcga caaagcctta
       61 ggacacacaa gtctgcatag ctgctttgaa gggacccacg gtgccatctg ttccacatgc
      121 gcgaaagaag gcgcatacgc atacaaaatg acgaagatgg gcaagactcc aaagcagatt
      181 cgtgaaggca tagagcacaa tgaatatcag acaattgacc tgaaccagct cggtggaatg
      241 taggcgtacg tctgctacac tcgccgcatg gcatgtggtt ttttccttac ctttgaagga
      301 ctggacggct ccggtaaaac aacgcagata cgcaaactga ccgcgcggct ggaatcgcaa
      361 ggacataaag tcatagtcac acgccaaccg ggaggcacac gcattggcga ccgcatccgt
      421 cagcttctgc tggattcccg gactgaaaat cttgcgcctc tggctgaact gggtctgatg
      481 ttctccgatc gcgcgcaggc catagcagaa gtcatccggc cggcgctcga tgcgggacat
      541 atcgtggtct gcgaccgtta caccgactcc acggaagcct accagggtgg agggcgacag
      601 cttggcagcg agattgtgct gaagctgcat aacgccatct gtgaaggact tcaacctgac
      661 ctgaccatcc tgcttcttcc ggattttgac cgttcacttc atcgtgcgcg ccgccgcaat
      721 gagcgaaacg caaagtcagg cgcagatgaa aaccgttttg agcgcgaggc agaagacttt
      781 tatcgtcggg ttcacgacaa ataccgggaa attgcgcagc gtgatgcgca acgggtcgta
      841 gcgattgaag gtgatgcggg aattgaagaa atccatgagc aaattgcgaa gattgttgaa
      901 gaacgcttga aacgttgaat gaccgtctat gactgacaga aattgcccct ggaatttcga
      961 cagaatcaac attctcaaaa atttcgccaa atagaagcgt gtggttacca tcagcatccc
     1021 tctatggttc ccattctgcg ccggaatgcg ttacagttca tctaataggg cagaagcgaa
     1081 aggaacattg tgacctcaaa agacgtgcga gataagaacg aacttcgcaa gctccccatg
     1141 atgccgattc gggacatggt gatcttcccc tacatgatga cgccctttgt agtgggccgc
     1201 gaaaccagtg tgcgcgccct ggaagaggca ctgaccggcg accgcaagat ttttctcgcc
     1261 acacagcatg atgcgcgagt ggatgagccg aaggcctcgg acatctactc tgtcggcacc
     1321 atcggcaata ttgtgcagag cgtcaaaatg ccggatggca atatcaaagt ccttgtggaa
     1381 ggactggagc gcgccaaagc tgttgaaatg aatgacgctg acggcttctt cgttgccacc
     1441 gtgcgtgttc ccaaaattga tattgagcgc gcgtccagca ttgagcagct tacccagcgc
     1501 gttacttccc tcttcgagca gtacatcaag ctgcagcaat cactgaatta cgaaaccatg
     1561 attgctgccg tgcgcacgga cgagcctggg aaactcgccg atacaatcgc agccaatctc
     1621 cagcttgaaa ttcctgaaaa gcaagagctg cttgagattt ttgatccggc cgagcgcctt
     1681 ctgcgccttg ccgatgtgct cgacatcgaa attgaaaagc tcaatatgga gcgcagcatt
     1741 cagtcgcgcg taaagcgcca gatggagcgc gcccaaaaag agtattacct caacgagaag
     1801 atcaaggcca ttcagaagga actgggccgc agcgaaaaga gcgaattcga tgatctgaag
     1861 aagaaaatcg aatcggccgg aatgcccagg ccggtctttg acaaggcgat tcaggagctg
     1921 aagaagctcg aggcgatgcc gcctatgtcg gcagaatcca ctgtctcgcg gaattacctg
     1981 gactggctgc tcgcggttcc ctggaagaag aaatctaaag aaacgcgctc cattgaaaaa
     2041 gccgagaagg tactcaacga agaccattat ggccttgaaa aaatcaagga gcgcattctg
     2101 gaattccttg ctgtccgcca gcttgtgaaa aatccaaaag gctcgattct ctgctttgtc
     2161 gggcctccgg gtgttggcaa aacctccctg ggtatgtcca ttgccaaagc gactggacgc
     2221 aagttcgtcc gcatgtccct gggtggcgtg cgcgatgagg ctgagattcg tggacaccgc
     2281 cgcacgtata tcggcgcgtt gccaggccag atcatccagt ccatgaagaa agcggggaca
     2341 aagaacccgg tcttcatgct cgacgaagtg gacaagatgg catccgattt ccgcggtgat
     2401 ccggcatcgg cgctgctcga agtcctcgat ccggagcaga accacagctt ccaggaccat
     2461 tatctggacg tcgagtatga cctttccgag gtactcttcg ttgccacagc caatgtgctg
     2521 cataccattc ccgctccatt gcaggatcgt atggagatcc tgcgcctgca cgggtacacg
     2581 gaagcagaga aactggaaat cgccaggcag tatctcatca aaaagcagcg cgagaatacc
     2641 ggactgaccg aaaagaacat cgtattcacg gatgaagcgc ttattgcaat gattcgcagc
     2701 tatacacgcg aggccggggt acgcaatctc gaacgcgaaa tcggcaatat ctgccgcaag
     2761 gtggcccgcc gcgtcgtaaa agctggagcg aagcacaagg aagagattac gcctgacaat
     2821 ctcgcagact ttctgggagt cgccaggttc cgcgattctc aggtgcatga gaaaagcgag
     2881 gtcggcctgg tcactggcct cgcctggacc gaggtcggcg gcagcatcct tcctgccgag
     2941 gtgcagattc ttgacggtaa aggaaagctt acgcttaccg gccagctcgg cgatgtaatg
     3001 caggaatcag cgcaggccgc tctttcctat gtccgcagcc gtgcgctgca tcttgggctt
     3061 tcgcgcgact tctatcgcaa tgtagacatc cacattcatg tgccggaagg cgctattcct
     3121 aaagacggcc cttcggcagg cattacgatg gccacggcgc tggccagcgc gctgacgaaa
     3181 ataccggtac ggcgcgacat cgccatgact ggagaaatta ccctgcgcgg caaagtcctg
     3241 cctatcggcg gactcaagga gaagctgctg gctgcccatc gcgccggaat ttttgaggcc
     3301 attctgccca gggacaacga aaaggacctg gccgagctgc ccgacaatct gaagagcgcc
     3361 atgaagctgc actttgtgga ctcgatggac gaggtgctgc aactggcgct cgaagggcca
     3421 ctgcctgagc ttagtgaaga gccagcggct gtgcttcctt ccgttcctcc gccggcagtc
     3481 actgcgccgg agcagcgtcc acaccaatag ttgaaaacca gagggtaggc catcctggcc
     3541 tgccctggat aaactatgaa taaactacta tggatgaatt cccccggcca gatgtctggc
     3601 agcagctttt cgctcttacc tgcttatttc tgagctaaaa agtagcgttc ggcgttctgc
     3661 cttgcgcgtt ccagtgcatc gttcaattcc tgccgcttgg cttcaagggc ggcagagtct
     3721 gcgtcctccg caggcggggc cacagggcgc gcccagctaa cagccacacg agaaaacggc
     3781 ctggggacca ggaagtggtc ccatgagcgc attgtccagg cgcgttcggg aagaagatag
     3841 aagctgccga tttcctgctg cgtcatctgt gcaagtttta ccggccccat ttttgtctga
     3901 tagatggggc cgcgcggccc gtcggcggta aagaccacgg gcagcccttg ttcaagcacc
     3961 tgctcaaggg caatcaatcc ggctgcaccg ccgcgcgagc ttgagccgcg cacgctgtca
     4021 aaacccagaa gcgccagagt gcgggcaatc agctctccat caaaacttcg gctgatgagg
     4081 atcgagcagc ggaatttgcg gaagtaccag gcacagggca gagtgcagcg gtgccagaaa
     4141 cagaagattc ccgttgccgg aggcgtcgcg ggcgttgccc ccggttctgc gatgacttca
     4201 aacctcagcg ttgctccgat gaccgcaagg actaccgctg tgaggcgcgg cacaatggcg
     4261 agggcgatgc gctggccaag agtgaatttt g
//
LOCUS       DASRBV010000062         6536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_458950, whole genome shotgun sequence.
ACCESSION   DASRBV010000062 DASRBV010000000
VERSION     DASRBV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458950"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFA02_02580"
     CDS             <1..960
                     /locus_tag="VFA02_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU08762.1"
                     /translation="EYAALKPHDTQHEVFLVLGPWNHGEWARTTRYLGDVDFGAATTD
                     QFRTGTEAPFFAHYLKDEGSFDLEDTASFQTGTNRWMRYSHWPPKESTGRNLYLDSDG
                     TLSFEKPKGDDATSFTSYVSDPANPVPYRRRPIEATYAPGGSGWYTWLVQDQRFLNGR
                     KDIATWTTPALDHDVTVTGDVIADLFASTSGTDSDWILKLIDVYPNDPSLGKMSGYEL
                     MIVDEIFRGRYRNSFEHPQAIPANQPEEYKFSLHGADHVFLKGHRIMVQVQSTWFPLY
                     DRNPQTFVPNIMTASPSDYKAATQRIYGSTAHPSHLVLPVIEK"
     gene            1231..4800
                     /locus_tag="VFA02_02585"
     CDS             1231..4800
                     /locus_tag="VFA02_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08763.1"
                     /translation="MCRSRLHQWMLCASILGFVLVFLLASATTSQAQNAQGTIVGHVT
                     DPSGAVVPGAKVAITDINTGVTHNYVTSATGDYTASDLNPGQYSVTVQATGFSQASSP
                     SLTLQVAQTVRQDFKLTVGAETATVTVSAAAQMLQTEDASVGQVISGELMQALPMNGR
                     DFTNLMITNIGTNLMQSGSGMDWSYHGLNQEYYEVSANGAEAQSASYSIDGIYDADFF
                     FSVPINIPNESAIQEFRMLNGMYGAQYGQGSAQVNVAVKSGTNRLHGGAYETFQANWL
                     EPDNKKQAVQNQLSGENLPLSTPFHQHQFGGYLGGPFWKNKFFWFGSYDQGLYNKVNA
                     GNTAFVPTAAEMSGDFSSYPYPIYNPQTTVPNPNYNPNSNDPIISSPVLRTPFAGNQV
                     PSGMIDTVAQKMAAFYPKPNITSCTEAVHVQNGCSNYAANTFTKKRQGVGTARLDEYI
                     GQNDHLFETVNIGTLSQTSTSIAFGQGGQVYTRPRLYGITWTHTFNANLLNQATLGYS
                     RDHFLTGQNTAYGPNLSAEVGLANTVPNPATFDLPLLQFTQGYQSMGGGEPTSYTDNI
                     YQGVDTVTLTHGRHTLNFGIDFRRIQLYEIDNYLGTGSLSFNGEYTALNPSLAGTSVS
                     QNGAYQGNAFADFMLGDTSSATGPPPLGTDNYNLWGNNINLFFQDDIKATSRLTINAG
                     LRWERPGNFHSTNNDGFAFSTANGGQFVWANCSFAQSILKAGGNPNYLQCGAVNTLVQ
                     IDKLDFAPRLGFAWRPPVTDRLVIRGGYGIFYDTYNRYYDGTQFDKNELYTEAAATYS
                     PGTGKETQSPAVVKSLWYAPTTSTQTFSAPSYQFPYNQVNWPGNHNPYNQQWNLDTEY
                     ALAPTLMLDVGYVGAHGVHQPAWNVIGAARPPKVAGDPCNNLADASLATGSNASCASD
                     PNFEPIDTRTPYPNMPPYLYANQNGYNTNYNALQTQLIERGAHGLTLHLNYTWSKTMD
                     ITSAYNLVNGEPDLLQDNFHPGRMYGLAASNEKHRLVATYAYEVPRHIFHSRLMNTLA
                     GGWTTSGVYQLGSGFPFAVYGNEPADQMAIAYGGRFLANSTYRKTPGFKSTLSHYFDT
                     GKYSNPTLGTYGNTNKSPEISPYMTNWDLSVGKTTTTFEGQSLLIRAEYFNVMSTWHY
                     NPMNFCCNTNVSSSSFGTMIDPSLGNVSLFSPYTLQLTAQYTF"
     gene            complement(5098..>6536)
                     /locus_tag="VFA02_02590"
     CDS             complement(5098..>6536)
                     /locus_tag="VFA02_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU08764.1"
                     /translation="SRQGNQYAGAEACMPQKAATSGLARELTFRDLVLFYLAGGLSLR
                     WIATAAAAGPSTVLVWVFACCFFFVPLAGSVLELSSRHPEEGGLYVWTQRAFGDFAGF
                     IAAWTYWMSNLPYFPAVLYFGAGSALFAAGRHGEHLANNSAYYLLFALGWLVVITGLN
                     IIGLKQGKWLNNVCAMGAWISVAVLMTLACAEAGKYGPATDFSLRQLIPHPGLKNAIF
                     WSTIFFAFGGCETGSFMGEEIRDARRTIPRALIFAGVVMALCYIAGTVAMLVALPSSS
                     INGVQGFASAIQSLSTKLQLPWLVAPVMVLIVLNSVGGAAAFLSSTSRLPFVAGIDRY
                     LPAVFGRVHSRWRTPWVAIGVYGLAGMLCALLSQAGSTVRSAYDVLVSMSILTYFIPF
                     LFLFASMIRLQGEPAAEGVIRIPGGRRAAVLLASVGLLTTLLTIVLSVVPPEEEPHKW
                     MAIVKVIGSTLFLVGAGVVVYLASERKR"
BASE COUNT         1525 a         2237 c         1536 g         1238 t
ORIGIN      
        1 gaatacgccg ccctcaagcc gcatgacacg cagcacgaag tcttcctcgt cctcggcccc
       61 tggaaccacg gcgaatgggc ccgcaccacc cgctatctgg gcgatgtaga cttcggcgcg
      121 gccaccaccg accagttccg cactgggacg gaagcgccct tcttcgccca ctatctgaaa
      181 gatgaaggca gcttcgacct cgaagacacc gcttccttcc agaccgggac caaccgctgg
      241 atgcgttaca gccactggcc cccgaaagaa tccaccgggc gcaaccttta tctcgactcc
      301 gacggcacgc tcagcttcga gaaaccaaag ggcgacgacg ctaccagctt caccagctat
      361 gtctccgatc ctgcaaatcc ggtgccctat cgcagacggc ccattgaggc cacctacgcc
      421 cccgggggtt ccggctggta cacctggctc gtgcaggacc agcgctttct caacggtcga
      481 aaagacatcg ctacctggac cacgcccgct ctcgaccacg atgttaccgt taccggagat
      541 gtcatcgccg acctcttcgc ctcaacctcc ggcacagatt ccgactggat cctcaagctg
      601 atagatgtct atcctaacga cccctcgctg gggaaaatgt ctgggtacga gctgatgatc
      661 gtggatgaaa tcttccgcgg ccgctaccgc aacagcttcg agcatcccca agccattcct
      721 gccaaccagc ccgaggagta caaattcagc ctgcacggcg ccgaccacgt cttcctcaaa
      781 ggacaccgca tcatggtcca ggtgcagagc acatggtttc cgctctacga ccgcaatccg
      841 caaaccttcg tcccgaatat catgaccgcc tccccatctg actacaaagc ggccacccag
      901 cgcatctacg gatccacggc gcacccgtcc cacctcgtcc tgccggtcat cgaaaaataa
      961 tccggtggaa gtgcggcgca ggggtctgtt gaccgaatct catgttgatg ccataaaact
     1021 ctccggccat cctttatctg ccggtcagcc cctgcgcccc ataagcccat aggccgcata
     1081 aatagttttt atggattagg taacgctacc attttcctgt tgactgcctc ctgtctatac
     1141 aggtaacctg cttttcacaa ccgcattttc atccagctcg acgcggtttc ttaaataaaa
     1201 aataagagca tcggaaagga ggaccctcct atgtgtaggt cccgtctgca ccagtggatg
     1261 ctttgtgcat ccattttggg attcgttctt gtatttcttc tggccagcgc taccacctct
     1321 caagctcaaa acgcccaggg aaccatcgtc ggccacgtaa cagacccctc tggcgctgtt
     1381 gttccaggcg caaaagtcgc aatcacagac atcaataccg gcgtgaccca taactatgtc
     1441 acgtccgcca caggcgatta caccgcctca gacctcaacc ctggccagta ctctgttacg
     1501 gtgcaggcca caggcttttc gcaggccagc tctcccagcc tcactctcca ggtggcacag
     1561 accgtccgcc aggactttaa gctgaccgtg ggtgctgaaa cggcaacggt aacggtatcg
     1621 gcagccgcgc agatgctaca aaccgaagac gcgtccgttg gccaggtcat ctctggcgag
     1681 ctgatgcagg ccctccccat gaacggccgc gacttcacca acctgatgat caccaacatt
     1741 ggcaccaacc tgatgcagag cggttccggt atggactgga gctatcatgg tctgaaccag
     1801 gagtattacg aggtcagcgc caatggcgcc gaggcccagt ccgccagcta ctccattgat
     1861 ggcatctacg acgccgactt cttctttagc gtccccatca acatccccaa cgagtctgcc
     1921 attcaggaat tcagaatgct gaacggcatg tacggcgccc agtatggaca aggctccgcc
     1981 caggtcaacg tcgccgtcaa atccggcacc aaccgcctcc atggcggcgc ttatgaaacc
     2041 ttccaggcca actggcttga gccggacaac aaaaagcagg ccgtacagaa ccagttgtcg
     2101 ggcgaaaacc tccccctcag tacccctttt catcagcacc agtttggcgg atacctcggc
     2161 ggtccattct ggaagaacaa gttcttctgg tttggcagct acgaccaggg cctctacaac
     2221 aaagtcaatg cgggcaatac cgccttcgtc cctacggccg cagaaatgtc cggggacttc
     2281 agcagctatc cgtatcccat ctataatccg cagaccactg ttcccaatcc taactacaac
     2341 cccaatagca atgatcccat catcagttcg cccgtcctgc gcacaccttt cgcaggaaac
     2401 caggtcccca gcggcatgat agacactgtg gcccagaaaa tggccgcatt ctatcccaaa
     2461 ccgaatatca cctcctgcac agaggccgtc cacgttcaga atggctgctc caactatgcc
     2521 gccaacacct ttacgaagaa gagacagggc gtcggcaccg cccgtctcga cgagtacatc
     2581 ggtcagaacg accatctctt tgaaaccgtc aacatcggca ccctctccca gacctcaacc
     2641 agcatcgcct ttgggcaggg cggacaggtg tatacccgtc cccggctcta tggcatcacc
     2701 tggacccaca ccttcaacgc aaacctgctc aaccaggcca ccctgggcta cagtcgcgac
     2761 catttcctta ccggacagaa caccgcttac ggcccgaatc tctccgccga agtcggcctg
     2821 gccaatactg tgcccaaccc tgccaccttt gatctgccac tcctgcaatt tactcagggc
     2881 tatcagagca tgggcggagg cgagcctacc tcctacacag acaacatcta tcagggcgta
     2941 gacacggtca cccttactca tggccgccat accctgaact tcggcattga tttccgccgt
     3001 atccaactgt acgagattga caactatctc ggtaccggct ccctgagctt taatggcgag
     3061 tacacggcac tcaatccttc cctggctggg acaagcgtct cgcagaacgg tgcctatcaa
     3121 ggcaatgcct ttgccgattt catgctcggc gatacctcgt ccgccacggg accaccgcct
     3181 ctgggaactg acaactacaa tctctggggc aacaacatca acctcttctt ccaggacgac
     3241 atcaaggcca ccagccgtct gaccatcaac gccgggctcc gctgggagcg ccctggaaac
     3301 ttccatagca cgaacaacga tggcttcgcc ttctctacag caaatggtgg gcagtttgtc
     3361 tgggccaact gtagcttcgc ccaatctata ctgaaggctg gcggtaaccc caactatctc
     3421 cagtgcggcg ccgtcaacac cctggtccag attgacaagc tggacttcgc tccccgcctc
     3481 ggctttgcct ggcgcccccc tgttaccgac cggcttgtca ttcgtggcgg ctatggcatc
     3541 ttttatgaca cctacaaccg ctactatgac ggtacccagt tcgacaaaaa cgaactctac
     3601 accgaggcgg cagctacata ctcccctggc acaggaaagg agacccagtc ccctgcggtc
     3661 gtaaagagct tgtggtatgc cccgacgacc tccacacaaa ccttctcggc cccgtcgtac
     3721 cagttcccct ataaccaggt caactggccc ggcaatcaca acccctacaa ccaacagtgg
     3781 aacctggaca ccgagtacgc cctcgccccc acccttatgc tcgatgtggg atacgtaggc
     3841 gcgcatggcg tccatcagcc tgcctggaac gtcatcggtg cagcccgtcc ccccaaagtt
     3901 gccggcgacc cctgcaacaa cctcgccgat gcctctctgg ccacaggatc gaatgcttcc
     3961 tgcgcctcag accccaactt tgagccgatt gatacgcgca caccctaccc gaacatgccg
     4021 ccctatctct acgcgaacca gaacgggtac aacaccaact acaacgccct gcagacccag
     4081 ctcatcgagc gcggcgcgca cgggctcacg ctgcacctga attacacatg gtccaagacc
     4141 atggacatca cctcggctta taacctggtc aacggcgagc ctgacctgct ccaggacaac
     4201 ttccatcccg gacgcatgta cggactggcc gcctccaatg aaaaacatcg tctcgtggcc
     4261 acctatgctt acgaggtgcc gcgccacatc ttccactccc gcctgatgaa tacgctggcc
     4321 ggaggctgga ccacaagcgg cgtgtatcag ttgggcagcg gctttccctt tgcagtctac
     4381 ggaaatgaac cagctgacca aatggccatc gcctacggcg ggcgcttcct ggccaactcc
     4441 acctatcgca aaacgccggg cttcaagtcc accctctccc attacttcga caccggcaaa
     4501 tactccaatc ccactctggg cacctatggc aataccaata aaagcccgga aatcagcccc
     4561 tatatgacca actgggacct cagtgtcggc aagaccacta cgacctttga gggccagtcc
     4621 ctgctcatcc gggccgagta cttcaatgtc atgtccacct ggcattacaa cccgatgaac
     4681 ttctgctgca acaccaatgt ttccagctcc agcttcggca ccatgattga cccgtcgctt
     4741 ggaaacgtct ccctgttttc tccatatacg ttgcagttaa cagctcaata caccttctga
     4801 gttgctctcc ctccggcgcc gcgacccgtt cgcggcgctt ttttttgcca atatgggcga
     4861 cgcggtaaat agagtccgtc attctgacca cagatataga gcctgtcagc ctgtccgacc
     4921 actctatata acccgtcatt ctgacgaccg aagggaggaa gaatcttagc gatgtaacgc
     4981 tgtagccaat cctgctaaga tccccccccc cgctgccaga aagtccggtt ttgaaggggc
     5041 acggctttta gccgccgagg gttcgatgca gggtttccgc cccgcccgga atgacgatta
     5101 tcgcttccgt tccgaagcca gatacaccac cactccggcc cccaccagaa atagcgtcga
     5161 gccaatcacc ttaacgatcg ccatccactt gtgcggctcc tcctccggag gaacaacgga
     5221 gagcacaatc gtcaacagcg tcgtcagcaa acccacggaa gccagcagca cagcggccct
     5281 tctgcctccg ggaatccgta tgacgccctc agcagccggc tcgccttgca gccggatcat
     5341 cgaagcaaac aaaaacagaa acggaatgaa ataagtaagg atggacatgc tcaccagcac
     5401 gtcatacgcg ctgcgcaccg tgcttcctgc ctgactgagc agcgcgcaca gcatgcccgc
     5461 cagcccatac acgccaatcg ccacccacgg agtccgccac cgcgaatgca cccgcccaaa
     5521 cactgccggc agatagcggt cgattccggc aacgaagggc agccgtgaag tggatgacag
     5581 aaatgccgct gctccgccca cgctgttgag cacaatcaac accatcaccg gggccaccag
     5641 ccacggcaac tgcaattttg tgctcagcga ctggatcgcg ctggcaaaac cctgcactcc
     5701 gttgatggaa gagctgggca gcgccaccag catggccacc gttccggcaa tataacagag
     5761 ggccatcaca actccggcaa agatcagagc ccgcggaatc gtcctccgcg catctctgat
     5821 ctcctcgccc ataaacgacc ccgtctcgca tcccccaaat gcaaagaaaa tcgtggacca
     5881 gaagatcgca ttcttcaaac caggatgcgg gatgagttga cgcagcgaaa agtctgtcgc
     5941 cgggccatat tttcctgcct ctgcacaggc cagtgtcatc agcacggcaa cagaaatcca
     6001 cgcgcccatc gcgcagacat tgtttagcca cttgccttgc ttcagtccaa tgatattcag
     6061 tccggtaatc actacaagcc atcccagcgc aaacagcagg taatacgcgc tgttgttcgc
     6121 caggtgctct ccatgccggc ccgccgcaaa tagcgcgctg cccgcgccaa aatagagcac
     6181 tgcaggaaag tacggcagat tgctcatcca gtacgtccac gctgcaatga acccggcaaa
     6241 atcgccaaag gcgcgttgcg tccagacata gagaccgccc tcctccggat ggcgcgatga
     6301 aagctccagc acgctgccgg ccagcggcac aaagaaaaag cagcacgcaa acacccagac
     6361 aaggaccgtg ctgggaccgg ccgccgccgc ggtagcaatc caacgcaggc tcagcccgcc
     6421 tgcaagataa aacaaaacca gatcgcggaa agtaagctct cgcgcaaggc cggacgtggc
     6481 tgctttctgt ggcatgcagg cttccgcgcc agcatactgg ttgccctgac gcgaag
//
LOCUS       DASRBV010000063         7610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_207982, whole genome shotgun sequence.
ACCESSION   DASRBV010000063 DASRBV010000000
VERSION     DASRBV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207982"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..2426)
                     /locus_tag="VFA02_02595"
     CDS             complement(312..2426)
                     /locus_tag="VFA02_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08765.1"
                     /translation="MTVALAPLLLLVAGCGVQLNGMNSAGLLISSPSASISTTGQLRF
                     KATLPDGSPATVSWAVTSSQTNASMGIGSIDATGLYTPPNALANDALRVQITARLQSD
                     PSRFATSIITVTPGFLQSLLPENAALTAGASLEISAQIAEVGGGSVHWNLSDNNVGAL
                     GPSTCSRGPHQYTVCRVMYTAPSLVSAQQSVHVIATVNDTATQSQLHLLLNNNGVNSN
                     ALTNQSTQSGPVVLGASGSNDTDYDVYKDGSGNSYIADCCGGTLGALVEDSAHNQYIL
                     SNNHVLAESDQAKVGDTIDQPGLIDNACRPLTSRPVGTLRYYVPLASPQTNVDAALAS
                     VVPGAVSSDGSILQFGTQGTGMNASLGSAPPMAGTGETLTADNLSGLRVVKSGRTTGL
                     TCSTVETVDLNVKVDYYKDCAETQPYYTKTFVGQIGISGDNFSDSGDSGSLVVDAGNA
                     QAIGLFYAGGTDGDGNGLSVVNPISDVLNELGAETGSKLSIVGTHTPHSVACLDYDKN
                     METPPALPALSATAQTQASHATLIASAALVNHEKGILGVAPGQSADGPGDPAVIVYVD
                     RNNPNTAVPKTIADVRTVVVPADAASVANDSAPKLPVTSSGIHLSASILNTAKAVVDR
                     YASQLMSDPAIFGVGVTQSHDDPREAALLVLIDMDRMPKSMPATIGGLRVRYMRLHRF
                     HVTKSKYAGAHPVSSCSLKSGN"
     gene            complement(2522..2803)
                     /locus_tag="VFA02_02600"
     CDS             complement(2522..2803)
                     /locus_tag="VFA02_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08766.1"
                     /translation="MKKILQAILDFFYEIFLGCRHSHLTRPFTIQHETYKVCLDCGKR
                     IYYSPETLLPLSAREVRRLEAARAGELKVVPIASSASALAGDHKPRIAA"
     gene            complement(2916..3926)
                     /locus_tag="VFA02_02605"
     CDS             complement(2916..3926)
                     /locus_tag="VFA02_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZU08767.1"
                     /translation="MKFWQIPAFGIDNLQLAEKPVPHPGPNEVLIKVHAVSLNYRDLM
                     ITLGKYNPQMHLPRVPASDGAGEVVAVGEGVTEIKTGDRVAGIFMQNWLDGPPTPAKH
                     RGALGGDRDGMLSEHVVLPESGVVRVPEHLSWEEAATLPCAGVTAWNAVVHAGNIKAG
                     DTVVIQGTGGVSIFALQFAKLLGARVLGTSGSDEKLARARKLGLDAGVNYKQNSDWAK
                     WVYEQTSGEGADLVVEVGGAGTLTQSLQAVRVGGTVAQIGVLSQSNEPLRIAPILHKQ
                     VHIQGIYVGSRADFLEMDRAIVQNHLQPVVDEIFGFTELPRALRRMESGAHFGKLVVR
                     VD"
     gene            4048..4248
                     /locus_tag="VFA02_02610"
     CDS             4048..4248
                     /locus_tag="VFA02_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08768.1"
                     /translation="MAERESAVHVEDGRPDAATEAARKRERQMLELQRERILDERTSN
                     PHRRAALEAALKDIESKLAAIS"
     gene            complement(4288..5064)
                     /locus_tag="VFA02_02615"
     CDS             complement(4288..5064)
                     /locus_tag="VFA02_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU08769.1"
                     /translation="MEKLKGKTVFITGASSGIGEATAHAFAAQGARLLLAARRLDRLN
                     AMQPELLEKGATAVHCFALDVRDNAQVEKQLSGLPAEWKAIDVLINNAGLSRGLEKVH
                     EGKIQDWEEMIDTNIKGLLYVTRAVVPEMVSRGSGHIVNLGSTAGHLTYPGGAVYCGT
                     KAAEQSINDGLRQDLLGTPVRVTSIDPGMVETEFSEVRFHGDKERAAKVYQGLTPLTP
                     SDVADAIVWAVSRPAHVNIARILMTSIDQANSLLFNRRQS"
     gene            complement(5188..6069)
                     /locus_tag="VFA02_02620"
     CDS             complement(5188..6069)
                     /locus_tag="VFA02_02620"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU08770.1"
                     /translation="MRIAFLGTGQMGSAIALRLLEHGHKLIAWNRTASRLKPLLEKGA
                     SQAASPQEAVKDSDVIFSMFMDDAAATGTVLDENLIDAMPEGAIHVSLSTISVQLSQQ
                     LTVEHSVRKRIFVAAPVFGRPNVAAEGKLWTVVAGRQDAVEKIRPLLDTFSRGVTIMG
                     EKPYKAHAMKIGGNFLITAMIASLSEGFVYAESQSIDPAQYAEVVNSALFRSPFYEAY
                     SKVMLNPPEVPGATIALGQKDMRLFRESAAATSTRTPLADHFAGQLHGAAEAGLRDRD
                     WAAGYYQFVRSISHNPE"
     gene            complement(6187..7164)
                     /locus_tag="VFA02_02625"
     CDS             complement(6187..7164)
                     /locus_tag="VFA02_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HZU08771.1"
                     /translation="MKKARHSILAITLFTLLSAGLVRAQEPNPATTYQWPQNKPSLEI
                     DNGVPVYRIQVVGREIPAINYFHRSGKTEVGFAGTPLLPNAKGSAEVESHLGRTSIHV
                     RFKGLKPANAFGVEYLTYVLWAITPEGRPVNLGEVLPDGSEAELTATTNLQSFGLIVT
                     AEPYFAVTMPSDVVVLQNQVLPDKTQGIVQTVNAHATLLPRGSYGQITGGQPSRELIT
                     RNDQWPLELYEAINAVQIAEAAGATKYASDTLMTAKQQLQNAMDLQNHDSQRKQEITY
                     ARAAVQTAEDARIITLRKKMAEEQARSNPAPVTSPAPSSPATDANTSPH"
BASE COUNT         1619 a         1949 c         2259 g         1783 t
ORIGIN      
        1 tgccattgcg gtgcttctcc ttcgcgcgag atttgctcgc ggtttttgag tactgaaatt
       61 caggaattac ttaagttctc gagagcagac gaggtgctcg atagaggttt agcacttcca
      121 ctctccgact gctaatgtag aaaagggacc ggagagttgt caagcggcga aatgaaaaat
      181 atgagttaaa atcactcaaa tattctgcgc catttatttg cgcagagaaa ggaaggtttg
      241 ctggcagacg gattccagaa taataacctc ttcttttcaa tagtttaaag ataaaactct
      301 aaggtgggtt tttaattgcc ggacttgaga gagcagcttg agaccgggtg tgctccagca
      361 tatttggatt tcgttacatg gaagcggtgc aggcgcatat agcgcacgcg cagaccgccg
      421 atcgtagctg gcatggattt gggcatcctg tccatgtcta tgagaacaag cagagcggct
      481 tcacgcggat catcatggct ttgggtcacg ccgactccaa aaatggctgg gtcggacatg
      541 agctgcgaag catagcgatc cacaactgcc ttcgccgtat tcagaatgct cgctgaaaga
      601 tggatacccg agcttgtgac tggcagcttt ggcgcggaat cattcgccac gctagctgcg
      661 tctgcaggaa cgacaaccgt cctgacatca gcgatggttt tcggcactgc tgtgttggga
      721 ttgttcctgt caacgtaaac aatgacggcc ggatctccgg ggccgtcggc gctttggccg
      781 ggggccactc ccaggattcc tttctcatga ttcacgagtg cggcagatgc gatcagcgtg
      841 gcatggctag cctgggtctg ggcagttgcc gacagggcgg gaagcgccgg gggcgtttcc
      901 atgtttttgt cgtaatcgag gcaggcgacg gaatgaggtg tgtgcgtccc gacaatgctc
      961 agttttgatc ctgtttctgc gccgagctcg ttcaggacat cgctgatagg gttaacaacg
     1021 ctcaggccgt tgccatcgcc gtcggttcct cccgcataaa agaggccaat ggcctgggca
     1081 ttaccggcgt ctacaaccag ggagccggag tcgccgctat cggagaagtt gtcgccgctt
     1141 atgccgattt gtccaacgaa ggtcttggtg taatacggtt gtgtttcagc gcagtccttg
     1201 tagtaatcca ccttgacgtt caggtctact gtttcgacag tcgagcaggt gaggcccgtc
     1261 gtgcgtccac tcttgacgac gcggagacca ctgagattgt ccgcggtgag ggtctctcca
     1321 gtcccagcca tgggaggcgc ggagccaagc gaggcgttca tgcctgtgcc ttgtgttcca
     1381 aattgcaaaa tgctgccgtc tgaacttaca gctccgggga cgacagaagc aagggccgcg
     1441 tccacgttcg tctgaggtga agccagcggc acataatacc tgagcgttcc gaccgggcgc
     1501 gaagtcagcg gacggcaagc gttgtcaatg aggccaggct gatcaatcgt gtcgccaacc
     1561 tttgcctggt cggactcagc gaggacgtgg ttgttgctca aaatgtactg attgtgcgcc
     1621 gaatcttcta ccagagcacc gagagttcct ccgcagcaat cggcgatata gctgtttccg
     1681 gagccgtcct tgtatacgtc gtaatcggtg tcgttgctgc ccgatgcgcc cagcactacc
     1741 ggtccggatt gcgttgactg gtttgtgagc gcattcgaat tcacgccatt gttgttcagc
     1801 agaagatgaa gctgggactg agtagctgta tcgttgacag ttgcaatgac gtggacggac
     1861 tgctgcgcgg aaacaagcga tggcgcggta tacatgacac ggcaaacggt gtattgatgc
     1921 ggtccacggc tacaggtgga ggggccaagc gctcccacat tgttgtcgct gagattccag
     1981 tggactgaac cgccgccgac ctcggcaatc tgggcggata tctccagcga agcgcctgct
     2041 gtaagagcag cattttccgg caggagggac tgaagaaatc cgggagtgac cgtgatgatg
     2101 ctggtagcaa agcgcgatgg gtcgctttgc aggcgcgccg ttatctggac gcgaagagcg
     2161 tcattcgcaa gcgcattggg gggcgtgtag agtccggttg catctatgga gccaataccc
     2221 atagatgcat tcgtttgcga agatgtaact gcccaggaaa ctgtggcggg tgagccgtcc
     2281 ggcagcgtgg ccttaaaacg aagctgtcct gttgtgctga tgctggctga cggcgaggaa
     2341 atcaaaagtc ctgcgctgtt catgccgttt aattgcacgc cgcatcctgc caccagcaga
     2401 agcaggggcg cgagcgccac agtcacacga aatctgcgaa aaaaagacat gcgtggcaaa
     2461 caggaggcca agctctatta gacattctgg ccttcccgtt tgtcactgtc gtgaataagg
     2521 atcaggccgc aatgcgcggc ttatggtcgc ccgcaagcgc agacgccgag gaggcaatgg
     2581 gtacgacctt cagttctccg gcgcgcgcag cttcaagacg gcgcacttcg cgcgcgctta
     2641 aaggcagcag ggtttccggg gaatagtaaa tgcgctttcc gcaatcaagg cagaccttat
     2701 aggtttcgtg ctggatggta aaggggcgcg taagatgcga atggcggcac cccagaaaaa
     2761 tttcatagaa gaagtccagg atggcttgca gaatcttttt cattgctggc cctttctcac
     2821 cttcattgtg tttatgaagg aagcggcgtt tcagctccaa caacaaatgg cttcgaccgc
     2881 atttcataag atgcagtttt ggccagaatg tcgctctaat ccacacgaac gacgagtttg
     2941 ccaaaatggg cccccgactc catgcggcgg agggccctgg gcagctccgt gaagccaaat
     3001 atttcatcca caacaggttg cagatggttc tggacaatgg cgcgatccat ttccaggaag
     3061 tccgcgcgtg agccgacata aattccttgt atgtggactt gcttgtgcag gattggagcg
     3121 atccggagcg gttcattcga ctggctcagt accccgatct gggccacggt cccgcccacg
     3181 cgaacggcct ggagtgactg cgtgagggtg cctgcgcctc caacctcaac aacaaggtca
     3241 gcgccttcgc cgctggtttg ttcatagacc catttggccc aatccgaatt ctgcttatag
     3301 ttgacccccg catccaggcc gagcttcctg gcgcgggcga gtttttcatc actgcctgat
     3361 gtgccgagga cacgcgcgcc cagcagcttt gcaaattgca gcgcaaaaat cgagacgccg
     3421 cctgtgccct gaatgacaac cgtatctccg gccttgatat tgcctgcatg gacaacggca
     3481 ttccatgcgg tcactccggc gcaggggagc gtggcggcct cttcccatga gaggtgctca
     3541 gggacgcgga caacgccgct ttccggcaga accacatgtt ccgaaagcat tccatctctg
     3601 tcgccgccga gcgcgccacg gtgttttgcc ggggtaggcg gaccatcgag ccagttctgc
     3661 ataaagattc cggcgacacg gtctccggtt tttatctccg ttacgccttc gcctacagca
     3721 acgacttcac cagcaccatc agaggcaggt acgcgtggca ggtgcatctg ggggttgtat
     3781 ttgccgagcg taatcatgag gtcgcggtaa ttcagcgaaa cggcatggac tttgattaag
     3841 acttcgttgg ggcctggatg cgggacgggt ttttccgcca gttgcagatt gtcaatgcca
     3901 aacgcaggaa tttgccagaa tttcatcttc tatctccgtt tcatgggtaa caaatgagat
     3961 gagcggaccg cctttcacga tgcgtccatg gagcgatacc ataaacgtat ggcgagagga
     4021 tgggaaagca aatcagtgga ggagcaagtg gctgaaagag aaagcgcggt ccatgttgag
     4081 gatggcaggc cggacgccgc taccgaagcg gcccgaaagc gtgagcgcca gatgctcgaa
     4141 ctgcaacgcg agcgaattct ggatgaacgc acatcgaacc cgcaccgcag ggccgccctg
     4201 gaagccgcgc tcaaggacat cgagtcaaag ctggcggcaa tttcttagtg ccctcccacg
     4261 tctttttttt atgacgtggg gaagcagtta cgactgcctg cggttgaaga gcaaagagtt
     4321 tgcctggtca atgctcgtca taagtatgcg ggcaatattt acatgcgcgg ggcgcgaaac
     4381 ggcccagacg atggcatcgg ccacgtcaga gggcgtgagc ggggtaagcc cctggtagac
     4441 ctttgctgcg cgttccttgt ctccgtggaa gcgtacttcg ctgaattccg tttcaaccat
     4501 tccaggatca atgctggtga cgcgcacagg cgtgcccagc aggtcttgcc gcagaccatc
     4561 atttatggac tgctcggcag ctttcgtgcc gcaatacact gcgcctccgg gatatgtgag
     4621 atgtccggcg gtagatccca gattgacaat gtggccgctg cctcgggaga ccatttccgg
     4681 tacgacggcc cgggtcacgt agagcaggcc cttgatgttg gtgtcaatca tctcctccca
     4741 gtcctggatt ttgccttcat ggaccttctc caggccgcgg ctgaggccgg cgttgttgat
     4801 gagaacatct atggctttcc attccgcagg caggccacta agttgttttt ctacctgcgc
     4861 gttgtcgcgc acatcaagcg caaagcaatg gacggcggtg gccccttttt caagcaactc
     4921 gggctgcatc gcgttcaggc ggtcaaggcg gcgggctgcg aggagcagac gcgcaccttg
     4981 cgcggcgaag gcgtgggccg tggcttcgcc gattcctgag cttgctccgg tgatgaagac
     5041 agtttttccc ttcagctttt ccatgaagtt atcgtagctg aggtggtaga ttcgccgtct
     5101 gttggagcaa aaaaaaagcc caaagcagcc caggttctac ccttgcctgt ctatctataa
     5161 gacagtttat tgaaattgtg cggaacatca ctccgggtta tgggagatgc tgcggacaaa
     5221 ctggtagtat ccagcggccc agtcacggtc gcgcagtccg gcttcggcgg ctccatgcag
     5281 ttgcccggcg aagtggtcgg ccagcggagt gcgtgtgctg gtggccgcag cggattcgcg
     5341 aaaaaggcgc atgtctttct gaccgagagc aatcgtcgct ccaggaacct ccggcggatt
     5401 cagcatgact ttggaatatg cctcgtaaaa gggagagcgg aagagcgccg aattgaccac
     5461 ttcagcgtac tgcgccgggt caatactttg tgattcggca taaacaaagc cctcgctgag
     5521 agatgcgatc atggcggtga tgaggaaatt tccgccgatt ttcatagcgt gggccttata
     5581 tggtttttct cccatgatgg tgacgccgcg gctgaaggtg tcgagcaaag ggcggatttt
     5641 ttcgacagca tcctgtctgc ctgcgacaac cgtccagagc ttgccttccg cagcgacatt
     5701 ggggcgcccg aagaccggtg ctgcgacaaa aatgcgcttg cgcacggagt gctccacggt
     5761 gagttgctgg gacaactgaa cgctgatggt gcttagtgaa acgtgaatgg caccctccgg
     5821 catggcatca atcaggtttt catcgagcac agttccagta gctgctgcgt cgtccatgaa
     5881 catgctgaag atgacgtcgc tatcctttac tgcttcctga ggggatgcag cctgcgatgc
     5941 tcctttctca agcagcggct ttaatctgga tgctgtccgg ttccaggcga tgagtttgtg
     6001 tccgtgttca aggaggcgca aggcaatggc gctccccatc tggcctgttc caagaaatgc
     6061 aattcgcata ggtgattaga ttcgcgtcag aaatagcatg tcatattttt gggcggccag
     6121 atttgtgggt ctcccatcct tttgcgcgaa aagcggcgca ggatgggaga ccctgggctt
     6181 caggttttag tgaggggagg tgttcgcgtc cgtagcagga gaagaaggcg ccggggaagt
     6241 cacaggagcg gggttagacc ttgcctgttc ttctgccatt ttctttctca gtgtgatgat
     6301 gcgggcgtct tccgctgtct ggacggcggc gcgggcatag gtgatttcct gcttgcgctg
     6361 gctatcgtgg ttttgcagat ccatggcgtt ttgcagttgc tgtttggcgg tcatgagcgt
     6421 gtcgctagca tatttggttg cgcctgcggc ttcggcaatc tgcacggcgt tgatggcttc
     6481 atagagttcc agcggccact gatcgttgcg cgtgatgagt tcacgcgaag gttgtccgcc
     6541 tgtgatttga ccataagagc cgcgaggcag cagtgtggcg tgtgcgttta ctgtctggac
     6601 gattccctgc gttttgtctg gcaggacctg gttctgcaaa acaacaacgt cgctgggcat
     6661 cgtcactgca aaatatggtt cggcggtgac aatgaggccg aaggattgca ggtttgttgt
     6721 ggcggtaagc tctgcttcgg agccatccgg caggacttcg cccagattga ctggacgtcc
     6781 ttcaggagtg atggcccaga gcacatatgt cagatattcg acgccaaacg cattggcggg
     6841 cttgagtcct ttgaacctga cgtgaatgga agtgcggccc aggtggcttt caacttcggc
     6901 ggagcctttg gcattgggca acaggggagt cccggcaaag cctacttctg ttttgccgct
     6961 gcgatgaaag tagttgatgg caggaatctc acggccgacg acctggattc tgtaaacagg
     7021 cacgccattg tcaatttcaa gtgagggctt attttgcggc cattgatacg tcgttgcagg
     7081 gttgggttcc tgcgctcgaa caagccctgc agaaagcaga gtgaaaagcg taatcgccag
     7141 aatggaatgt cgtgctttct tcatcgtcct tgctccttca ttaaaaaagc gaaaagcggc
     7201 aaactatgcc cagagcggac cctcctggca cattgccgca gttgttaaag atgcgttttt
     7261 catggccgaa gatatccgga aaattcaagg gaaaattttt gggtgaggca accaaaattg
     7321 aaacgacagt ggggccttcc ttgcaaaagt agaggtgaag tgccggcctg gaattgatgg
     7381 aaaaaggtga ataccgtggt tgccgcgcat ttaccttctg tttacaggac attcaaaaac
     7441 tgttttcatt gcgccgttaa ggtgaatgta tgcaatcaca tcattctggt cagttccgcc
     7501 tgcttctggc aggcattgtt ctgtttttgt tctctttgtc tgccagtgcg cagttcgata
     7561 atggaagcct tgtgggcacc attcgagact caacaggcgc gagtattgct
//
LOCUS       DASRBV010000064        13574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_664121, whole genome shotgun sequence.
ACCESSION   DASRBV010000064 DASRBV010000000
VERSION     DASRBV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664121"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1088
                     /locus_tag="VFA02_02630"
     CDS             3..1088
                     /locus_tag="VFA02_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU08772.1"
                     /translation="MLACVLVPAFAFAQSANTPVRVAIVGLEHGHVAGFLHQFPLQHD
                     AELVAIVDSDKALTARYQQQFHLDPKLFYSTLDQAVAATHPQAVLVYTSVGEHRYVIE
                     QAAQHGLSVMVEKPLTISLADAIAIRHVAEQHHIQVLVNYETTWYASNRAVYQEINNG
                     KLGDLRKVVIHDGHEGPKEIGVQPEFLKWLTDPAQNGAGALYDFGCYGADLMTWWMRG
                     ETPLSVTAVAQTDKPETYPRVDDDATIILRYPHAQAVLMPSWNWPFSRKDSEVYGTQG
                     YLITVGPDKLRARYTGEKQEQEIAAPPLVPPQDTSLHYLAAVLRGELQPKADLTSLDT
                     NIIVMQILDAARESARTGRTVRLKPLP"
     gene            complement(1090..1365)
                     /locus_tag="VFA02_02635"
     CDS             complement(1090..1365)
                     /locus_tag="VFA02_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZU08773.1"
                     /translation="MNGWKLWAVFAIGVAAGATVALLYAPQSGERTRRQVRRKLEDAA
                     DYVRDAADTIGERATTAYKAGREAVEDALDSASSVYNTAAKRVQSIV"
     gene            1593..3161
                     /locus_tag="VFA02_02640"
     CDS             1593..3161
                     /locus_tag="VFA02_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU08774.1"
                     /translation="MRVSQPPVAVIIGAGPAGLTAALEFVRRSNVRPIVLEASHEIGG
                     ISRTIRYKGNRMDIGGHRFFSKSDRVMNWWTELLPVSADEDGEHSIRYQNKERTVTAS
                     DAVSNPDRVMLVRPRKSRIYFLRSFFDYPLSLNLQTIRQLGMWRMFKIGIGYLHARLF
                     PRKKEETLEDFLINRFGTELYRTFFKSYTEKVWGVPCDRISAAWGAQRIKGLSLRSAI
                     AHFLKKTFTRRKPSDIAQKQTETSLIEKFLYPKYGPGQLWEYVAELVTEKGGCVQLGW
                     RADKLRTSGDRITAVEAVSDAGERRVIQADYVFSTMPVRELIRSLDAPVPDEIRTISD
                     GLIYRDFITIGLLVNKLSVTEPDGSPLRDTWIYIQEPDVLVGRLQIFNNWSPYLVSDP
                     DKVWIGLEYFCYDSDELWRMPDDAIKNFAISEVEKIGILKASDVLDGHVVRVPKTYPA
                     YFGTYDRFDEIIRYMDRYENLFLVGRNGMHKYNNQDHSMLTAMTAVDQILAGKIDKSI
                     LWQINTEQEYHEEK"
     gene            complement(3170..3988)
                     /locus_tag="VFA02_02645"
     CDS             complement(3170..3988)
                     /locus_tag="VFA02_02645"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZU08775.1"
                     /translation="MLFSTEQEQAIAARLRYYRDMGVFDFYRRGNASVVASEDETASE
                     ISAQTIEETMPKTEIMTGAAEDFLTAQPGPVPPEQRAEALRVIQEDIGACTRCPLAFL
                     GRHTIVFGEGDPNARLMFVGEGPGADEDAQGRPFVGRAGQLLNNMIAAMGLKREQVYI
                     ANIVKCRPPQNRTPEPVEANTCTQFLFRQMDVIRPEVIVALGSTAATYLLGSKSSLNA
                     LRGRLHEVRGARLIITYHPAYLLRDPRQKKEAWKDLQMAMAELGLPVPKRSAEE"
     gene            complement(4193..5410)
                     /gene="coaBC"
                     /locus_tag="VFA02_02650"
     CDS             complement(4193..5410)
                     /gene="coaBC"
                     /locus_tag="VFA02_02650"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZU08776.1"
                     /translation="MKVTVGVSGGVAAYKAAELVRALQRQALDVHVVMTEAAQQFIQP
                     LTFASLTGHKVITGLWAGDPDEGHLASAIEHIDAATSTDALVVAPATANILAKFSQGI
                     ADDFLTTMYLATTAPVIIAPAMNVNMWNHPATQANLALLEGRGVRIVSPESGYLACGM
                     IGSGRLAETEMIVQTVLDVLHHRNDLAGETVLITAGGTREALDPVRFLGNRSSGKMGH
                     ALAEAAVRRGAKVILVSAPTALRPPLGCEVVSVTTTEEMRAAVLGRMEEATIIIKAAA
                     VADYRPVIQAEQKLKRTGPLTIELEPTEDILAEVVQRRHEGLLVIGFAAETENALAHG
                     RDKLLRKGADAIVLNDVSREGIGFDSDRNAVTFLTKQTAIEFPEMTKRELADRILEEV
                     MALRRPQKVVAER"
     gene            complement(5509..8055)
                     /locus_tag="VFA02_02655"
     CDS             complement(5509..8055)
                     /locus_tag="VFA02_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08777.1"
                     /translation="MQAIFEDIRYALRQFGKAPGFTITAILTLALGIGATTAIFTLVH
                     AVLLKSLPVTKPEELVRVGDHENCCINGGMQDNWSLFSYDQYKLFRDGNPEFLQLAAF
                     QSGRDQISVRRQGSGHPAESFISEFVSGNAFDTFGIQAYAGRLFRPSDDHKGAAPVAV
                     MSFRTWQQKFGQDPSVVGASFVINGFPYTIVGITPPGFYGDRLTNDPPSFWLPLSSEP
                     QIDGVMAVLERPDLDWLNVTGRIRPGTNLKQLEAKMQVQLRQWMLSPQSKVEQRDRSL
                     IPKQTLHLAPGGSGVQSMRDEYKDGLHLLMWISSFVLLIACANLANLMLVRAATRRQQ
                     TSIRSALGAPRSRLVRQALTESVVLAILGGIAGLAVAYGGSKLILHLAFQKSYVPIHP
                     TPSLPVLGFAFAVSLVTGMLFGVAPAWMMAHAHPIDALRGANRTTAQGSTIAQKSLVV
                     FQAAISLVLLCAAGLLTQSLRNMQRQHFGFETRNRYIVHIDPQMAGYKPEQMDSFYRQ
                     LHDLLAAIPGVKRAAYSLYSPMEGNNWGTGVFIEGQAPPPPGSNENNASWVRASADYF
                     DAVGTKIIQGRGISESDTEASQPIAIVNKTFAKKFFKDGNAIGKHFGIDGPEHTGTFE
                     IVGVTEDTQYWSPTEKIRPMFFLSAHQWVKYNDPDMNTFENVSHSKMDSIEIETRGFV
                     PGLEAQVRRAIAQVNPDLTVIDFNTFADQVKGNFNQQAMITQLTSLFGLVALALASIG
                     LYGVTSYSVERRTSEIGIRMALGADRLAVLKLVLKGAFLQVILGLAIGIPATIFGGRA
                     LSSQLFGIKPYDPVVLLLTTIVLLASAFVAAVVPARRAANTEPMQALRTE"
     gene            8306..9142
                     /locus_tag="VFA02_02660"
     CDS             8306..9142
                     /locus_tag="VFA02_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08778.1"
                     /translation="MNSFLAARVLLCAAALIPLNMSGHAQIHNTMPVVDASAPPSAVR
                     QMPASALTPPRPFSRFAFSGEASTLGVRLQTAAYLDPHLNLRASGSILGFSDTISTNG
                     FQVDTKLHLSSAGMSLDYFPFPAHGWRISPGLLFYNANGADMTFTALPGTSFSLNNYT
                     YYSSSSDPVKGIGGVGLHSHNPAFTITTGWGNMVRPKGHFSFPVEVGVALIGAPTFNM
                     ALTSGQVCDANGQNCVNVATDPDVQANLQAQVSKYRKDIEPLKTYPVVSFGVAYTFRR
                     QY"
     gene            complement(9143..10273)
                     /locus_tag="VFA02_02665"
     CDS             complement(9143..10273)
                     /locus_tag="VFA02_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08779.1"
                     /translation="MKILSPTDSIGPAFRRTREVMLKPFRLGFFLKIALVAALTQPSF
                     YSVVISYPMQGAQAAAGAAAAQHQHRMDFSTLWQPGFGTVAGLVILAIGLLVGLVVWV
                     GITYLFCRLRFTLFDLIVYKQGKVRQAWSKYGQQSWRYFGVMLLASLAFLIVAAVAIG
                     PLFLRMIRTMKELALQGAASNPFPVFAAMFPILGIVLLLGLLWMIVDTVLQDFVLPPM
                     AIEDAPIEAAFGRFFSLLRDEPGTFLGYLLLRFVLSIGLSWALLVVVGIFLLIAGLGV
                     VLGGVLLFHLMWHGGIALQVVFIILAAIVALILLALYFAAMIAIYGTVAVVKESYAAY
                     FFGSRYQELGNRLEPPEEEAVAVRAEPPLPPLPPLQEPPPVW"
     gene            10552..11139
                     /locus_tag="VFA02_02670"
     CDS             10552..11139
                     /locus_tag="VFA02_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU08780.1"
                     /translation="MFIRWRTHLTLLLLAVLGLVPALLAQSDSSSISGTITDSAGAVV
                     PRATVTIRNEATSESRTATSNDAGFYTVSSLPPGLYTIQIEAQGFQGFVQQHTHLDPS
                     IGLRVDAALKVGSSSTTVNVVANANVVQTETAAVGQLVTRQQVQAIQLNGRNPIYLAQ
                     MEPGVRRGNSMSAFGFGLDNPFNINGSNYRENGQI"
     gene            11152..>13574
                     /locus_tag="VFA02_02675"
     CDS             11152..>13574
                     /locus_tag="VFA02_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08781.1"
                     /translation="MVRSRGNGTSVGVADVDSTSEMQVLTAAYPAEYGRSSGGLIRIV
                     PRSGTSEFHGALFEYLRNSFFNANTWIRKQPANPANIRNHPQAFRFNQFGWNFSGPVY
                     IPGLHFNEGRHKLFFLAGQEYLRYRHDDTVTRKVPTNLMRTGNFSELLSPNIFYNSTV
                     QLVDPNTGQNYNGNIIPPSELSPNGLGLLNAYPAPNAVNPSYNWIDAAIYPEDQRKDT
                     LVVDYIPADNHHLRFSLLNYNYDSVSPHYGNFNRTPQVWHRPNQVAVFHYTWTINPTT
                     VNDAFVSGSADHVRIGYDLSKGLYDRTKYGINYPYLFPAGDKEIPNKIPTINIANFDT
                     LDGGPYPSHSGGVVWDIGDSLTKVIGKHTLKFGFLYERESQNDFDQINVSSTTPGATN
                     NQNGFFRFTDTHGGAPTSHAAVANAALGLFDTYGEIGVRSYTVYLSNMYEYFAQDTWR
                     ATPRLVIEYGVRHSIMLPYYAKWGNQSFFSTSVYDPTTAPTIDPTTGFVSGSNLMDGI
                     VIPGKGFPGSAKGHVPDSILNNSYYQTLFHGFDRGYNPLVWTNIQPRVGITYSLDPQT
                     VIRAGGGRFFDRLGISDTVHLGGNPPFQPSGTVSFGSVDAPGGVGTNNYPINMTSYAY
                     HFPSPEAWSWTVSAEHEFPQIGIFTLGYVGRRGIHLQHLENINQLLPGTLINTSVSKP
                     DTLRPYQGYSVIQQQTNDGRSIYHSLQADLRRRVTHGLMFGIAYTWSKSLDSTSDAGN
                     NIPNYFDPMEYWGPSDYDTRHIVVVNYVWDIPYASHASNWAVRNVFGRWQLSGTSQFQ
                     SGSPLSVSTG"
BASE COUNT         3110 a         4285 c         3329 g         2850 t
ORIGIN      
        1 ttctgctggc ctgcgttctg gttccagcct tcgcctttgc ccaatctgca aacacacccg
       61 tccgcgttgc tatcgtcggc ctcgaacacg gacacgtggc cgggttcctc caccagttcc
      121 ctcttcagca cgatgccgaa ctagtcgcca tcgtggattc tgacaaagca ctcactgcac
      181 gctatcagca gcagtttcac ctcgacccaa agctcttcta cagcacgctc gatcaggccg
      241 tggccgcgac ccatccgcag gccgtgctcg tctatacctc cgtaggcgag caccgttacg
      301 tcattgagca ggccgcccag cacggcctga gcgtgatggt cgaaaagcct ctgaccatct
      361 ctctcgccga cgcgatcgcc atccggcatg ttgcagaaca gcaccacatt caggtgctcg
      421 tgaattatga aaccacctgg tatgccagca accgtgccgt ctatcaggaa atcaacaacg
      481 gcaaattggg agacctccgc aaggtcgtca ttcacgacgg acacgaaggc ccaaaggaaa
      541 tcggcgttca gcccgagttt ctgaagtggc tgactgaccc ggcacagaac ggcgccggcg
      601 cgctctatga tttcggctgc tatggtgccg atctgatgac ctggtggatg cgcggagaaa
      661 ctccactcag cgtgaccgca gttgcgcaga ccgacaagcc ggagacctat ccgcgtgtgg
      721 acgatgacgc cactatcatc ctccggtacc ctcacgcgca ggctgttctg atgccttcct
      781 ggaactggcc cttttcacgc aaagattctg aggtgtatgg gacccagggc tatctcatca
      841 cggtcggccc ggacaaactc cgggcccgct atacaggaga gaaacaggag caggaaattg
      901 cagcaccgcc gctcgtccct cctcaggaca cttccctgca ctatcttgct gccgtgctgc
      961 gcggcgagtt gcagccaaaa gctgacctca cctcgctcga caccaacatc atcgtgatgc
     1021 agattctcga tgccgcccgc gaatcagcgc gcacaggacg caccgtccgc ctgaagccgc
     1081 tgccataaat cagacgatgc tctgcacccg ctttgccgcc gtgttgtaca cgctgctggc
     1141 cgagtccagc gcgtcctcaa ccgcttctct gcccgctttg tacgccgtcg tggcgcgctc
     1201 accaatcgtg tctgccgcat cgcgcacgta atcggcagcg tcctccagct tccgccgcac
     1261 ctgccgccgc gttctctcac ccgattgcgg agcatacaac aaggcaaccg tcgctccagc
     1321 cgcgactcca atggcaaata ccgcccacag cttccatcca ttcatggcgc atctccttaa
     1381 gacatagacg ctactcgaaa tgagacgcaa atttgcgccc agggtttgct gcgtaaacac
     1441 acatctttcc ccgtgcgtct atacaataaa agttatagac agaaagaaaa ggacatcttt
     1501 caaccaagta acttcatgca gatttgttat tttttccgcg tcatgcggtt cctggatagc
     1561 gcgtcagagt gccttgctct ctttcacgga gcatgcgcgt gagccaacca cccgtcgccg
     1621 tcattattgg agctggccca gccggcctta ctgccgccct ggagttcgtc cgccgctcca
     1681 atgtccgccc cattgtgctt gaggccagcc atgaaatcgg cggcatctcg cgcaccatcc
     1741 ggtataaagg caaccgcatg gacattggcg ggcaccgctt cttctccaag tcggaccgcg
     1801 tcatgaactg gtggacagaa cttttgcccg tcagcgcaga cgaagacggc gagcactcca
     1861 ttcgttatca gaacaaagaa cgcaccgtga ctgcttccga tgctgtatcc aatcctgacc
     1921 gcgtcatgct cgtgcgtccc cgcaaaagcc gcatctattt tctgcgcagc tttttcgatt
     1981 acccgctcag cctcaatctc cagaccattc gccagctcgg aatgtggcgc atgttcaaaa
     2041 tcggcatcgg ctatctccat gcccgccttt tcccccgcaa aaaagaagag acactggaag
     2101 acttcctgat aaaccgcttt ggaacagagc tttacaggac tttcttcaag tcttacacgg
     2161 agaaggtctg gggcgtcccc tgcgaccgca tcagcgcggc atggggcgcg cagcgcatca
     2221 agggactctc cttacgctcg gccatcgcgc attttctcaa aaagaccttc acgcgccgga
     2281 agccctccga tatcgcgcag aagcaaaccg aaacctcgct gattgaaaaa tttctttatc
     2341 ccaaatatgg cccggggcaa ctctgggaat atgtagctga actggttaca gaaaaaggcg
     2401 gctgcgtcca gcttggctgg cgcgcagaca agctccgcac ttccggcgac cgcatcacgg
     2461 cggtggaggc agtcagtgat gcgggcgaac ggcgcgtcat ccaggccgat tacgtcttct
     2521 ccaccatgcc cgtgcgcgaa ctcattcgca gcctcgacgc tcccgtcccc gatgaaattc
     2581 gcaccatcag cgacggcctt atctatcgcg actttatcac catcgggctg ctcgtcaata
     2641 aactttccgt cactgaaccg gacggctctc cgctcagaga cacctggatc tatattcagg
     2701 agccggacgt ccttgtaggc cgcctgcaaa tcttcaacaa ctggagcccc tacctcgtct
     2761 cagaccccga caaagtctgg atcggcctcg aatacttctg ttatgactcg gacgagctgt
     2821 ggcgaatgcc cgacgacgcc atcaaaaact tcgcgatttc tgaggtggag aagatcggca
     2881 tcctgaaagc gtccgatgtt ctcgacggcc atgtcgtgcg cgtgccgaag acctatccag
     2941 cctatttcgg cacctacgac cgtttcgacg agatcatccg ctatatggac cgctacgaga
     3001 acctctttct tgttggccgc aacggcatgc acaagtacaa caaccaggac cactccatgc
     3061 tgacggccat gaccgccgta gaccagattc tcgccggaaa aatagacaag agcatcctct
     3121 ggcagatcaa caccgagcag gagtaccacg aagagaagta gcttttcttt tactcctcgg
     3181 cagaacgctt cggcaccggt agtccaagct ccgccatggc catctggagg tccttccagg
     3241 cttctttctt ctggcgtgga tcgcgcaaca ggtatgcagg atggtaagta ataatgagcc
     3301 gcgctccccg cacttcatga agacgtccgc gcagtgcgtt cagcgacgac ttgcttccca
     3361 gcagataagt ggccgcggtg gatccaagcg ccacaatcac ctcgggacgt atcacatcca
     3421 tctgcctgaa cagaaattgc gtgcaggtat tggcttccac aggctcaggc gtccggtttt
     3481 gcggcggacg gcatttgacg atattcgcaa tgtagacctg ttcgcgcttc agtcccatcg
     3541 ccgcaatcat gttgttgagc aattgcccgg cgcgtcccac aaatggacgc ccttgcgcat
     3601 cttcatctgc cccgggtcct tcgcctacga acatgagccg ggcattcgga tcaccttccc
     3661 cgaacacaat cgtgtgacgc ccaagaaagg ccagcggaca gcgcgtacac gccccgatgt
     3721 cttcctgaat cacgcggagg gcctccgccc tttgttccgg agggacaggg ccaggctgag
     3781 cggtaagaaa gtcttcggca gcgccggtca taatctcggt ctttggcatc gtttcttcaa
     3841 tggtttgcgc tgaaatttca gaagcagtct cgtcttcact tgcaactaca gatgcattcc
     3901 cgcgacggta aaaatcaaag acacccatat cgcggtaata gcgcaaacgt gctgcaatgg
     3961 cctgctcttg ttctgttgaa aacagcatgg aaagaatttc tattctacag cgtgtttcat
     4021 aaactggatg cgatacagcg tttggtgaat gcaaagttct gcgtgaatgc ctaatccatc
     4081 attctggcgg tccgcaggga ggaagaatct tagcgatgtg ggccttagcc acggccctct
     4141 ttgaaaaatt ttctgattca aggcccacgg gcgaaaacac aatcatgctt ccctaccttt
     4201 cggccacgac tttttgcggc cttcgcaacg ccattacttc ttcaagaatg cgatcagcca
     4261 gctccctttt cgtcatttca ggaaactcaa tggctgtctg ctttgtaaga aaggtcacgg
     4321 catttcggtc tgaatcgaag ccaattcctt cgcgcgaaac atcattcaga acaatggcgt
     4381 ccgcgccttt gcgcagcaac ttgtcgcggc catgcgccag cgcattctcc gtctcggctg
     4441 caaatccaat caccagcaga ccttcgtgcc tgcgctgcac aacttcggca agaatgtctt
     4501 ctgtcggctc aagctcaatg gtcagcgggc cggtacgctt cagcttctgc tcggcctgaa
     4561 tcacaggacg gtaatcagcc accgccgcag ctttaataat gatggtggct tcctccatcc
     4621 gtccaagcac agccgccctc atctcctctg tggtggtcac agaaaccact tcgcagccca
     4681 gcggcggacg caacgccgta ggcgcgctta ccagaatcac cttcgcgcct cgccggactg
     4741 cggcctctgc cagcgcatgg cccatcttgc cgctcgaccg gttgccgaga aagcgcaccg
     4801 ggtcgagggc ttcgcgggtg cctcccgcag tgataagcac agtctctcca gcaagatcat
     4861 tgcggtgatg cagcacatcc agcaccgtct gtacgatcat ctccgtttcc gccagacgcc
     4921 cgctcccaat catgccgcag gccaggtatc cactttccgg cgaaacgatg cggacgccgc
     4981 gcccttcaag cagcgcaagg ttcgcctgcg ttgccggatg gttccacata ttcacgttca
     5041 tcgccggagc gatgatcaca ggcgcagtcg tcgccaggta catcgtcgta aggaaatcat
     5101 cggcaattcc ctgcgaaaat ttggccagaa tgttggccgt cgccggagca accaccagcg
     5161 catctgtgga agttgcagca tcaatatgtt caattgctga ggccagatgg ccctcgtccg
     5221 gatcacccgc ccaaaggccc gtaatgactt tgtgtccggt aagagaggca aatgtaagcg
     5281 gctggatgaa ctgctgcgcg gcctcagtca tgaccacgtg aacatcaagc gcctgccgct
     5341 gcaaggcccg cactagttcc gcggctttgt aggccgcaac gcctccggaa accccgaccg
     5401 tgactttcat agttaagaag attttacgct ggattcataa tggatagctc cccggaagct
     5461 gccctttcgg ggctgaggaa aacccgtaca caactatgaa atattgcctt actctgtcct
     5521 gagagcttgc attggctcgg tgttggctgc acgccgggca ggaacaacag cggcgacaaa
     5581 ggcagatgcc agcagaacaa tcgtagtcag caacaatacc accggatcat agggtttgat
     5641 tccgaagagt tggcttgaca gtgcccgccc gccaaaaatg gttgccggaa tgcctatcgc
     5701 caacccgaga atcacctgta gaaacgcacc tttcagcacc agcttaagga cggccagacg
     5761 gtcggcacct aatgccatcc ggatgccaat ctcgctggtg cgcctctcaa ccgaatatga
     5821 cgtcacgccg taaagcccga tagaagccag ggccagcgct acaagaccga aaagtgacgt
     5881 caattgcgta atcatcgcct gctggttgaa gttgcccttc acctgatcgg caaaagtatt
     5941 gaagtcaatg accgtcaggt caggattcac ctgagcaatg gcccgccgca cctgcgcctc
     6001 cagtccggga acaaagccgc gtgtttcaat ctcaatcgaa tccatcttcg agtgtgagac
     6061 attctcaaac gtgttcatgt ccgggtcgtt atatttcacc cactggtgcg cggagagaaa
     6121 aaacataggg cggatttttt ctgtggggct ccaatattgt gtgtcttctg ttacaccaac
     6181 gatttcaaat gtgcccgtat gctccgggcc atcaataccg aaatgtttgc cgatggcatt
     6241 gccgtcctta aaaaacttct ttgcaaacgt cttgtttacg atggcgatgg gttgcgatgc
     6301 ctcagtgtcc gactctgaaa tgccgcggcc ctgaatgatc tttgtcccga ctgcatcgaa
     6361 atagtccgcg ctggccctta cccaggaagc attgttttca ttgcttcccg gaggcggagg
     6421 ggcctggcct tcgataaaaa cacccgtgcc ccagttgttc ccctccatcg ggctgtacaa
     6481 cgaatacgcg gcgcgcttta cgcccggaat cgccgccagc agatcatgca gctggcggta
     6541 aaaggaatcc atctgttcag gtttgtagcc cgccatctgc gggtcaatat ggacaatgta
     6601 gcggttacgt gtctcaaagc cgaagtgctg cctctgcata tttcgcagac tctgtgtaag
     6661 cagccccgca gcgcacaata atacgaggga aatggccgcc tgaaacacaa cgagcgactt
     6721 ttgcgcaatc gtgcttccct gcgccgtcgt gcggttcgct ccacgcaatg cgtctatcgg
     6781 atgcgcgtgc gccatcatcc atgccggcgc cactccaaag agcatgcccg ttaccagcga
     6841 tacagcaaaa gcaaatccca gcacaggaag cgaaggcgtt ggatgaatcg gcacatagct
     6901 cttctggaac gccagatgaa gaatgagctt cgacccgcca taagcgaccg ctaatccggc
     6961 tatgcctccc agaatggcaa gcacaacact ttccgtcagt gcctgacgca ccagccgcga
     7021 acggggagcg cccagcgctg accggatgga agtctgctgg cgccgggtcg ccgcgcgtac
     7081 cagcatcaga ttggcaagat tggcgcatgc aatcagcaat acgaacgagg aaatccacat
     7141 caacagatgc agcccgtcct tatactcgtc gcgcatgctc tgcacgccgc tgcccccggg
     7201 agcaagatgc agggtctgct tcggaatcag cgagcggtca cgctgctcaa ccttgctttg
     7261 cggacttagc atccactgcc gcaactgcac ctgcatcttt gcttcaagct gtttgaggtt
     7321 cgttcccggc ctgatgcgtc cagtcacgtt cagccagtcg agatcaggcc gttcgagcac
     7381 cgccatcact ccgtctatct gcggctcgct tgaaagcgga agccagaacg aaggtggatc
     7441 attcgtcagc cggtcgccat aaaatcccgg cggcgtgatt cccacaatgg tgtaaggaaa
     7501 tccattgata acaaaactcg ctcccaccac cgaagggtct tgcccgaatt tctgctgcca
     7561 tgtgcggaag ctcatcactg caactggggc cgcgccctta tggtcgtccg acggacggaa
     7621 cagacggccc gcataggcct gaatgccaaa cgtatcgaag gcattgcccg agacaaactc
     7681 gctgataaaa ctttctgctg gatggccact tccctgccgg cgcacgctga tctggtcgcg
     7741 gcccgactga aaggccgcaa gctgcagaaa ttcgggatta ccgtcgcgga agagcttgta
     7801 ctggtcatag gagaagagcg accagttgtc ctgcatcccg ccattgatgc agcagttttc
     7861 atggtctcca acgcggacca gctcttccgg cttcgtgacc gggagcgact tcagcagcac
     7921 ggcgtggacc agcgtaaaga tggccgtcgt cgcgccgatt cccagcgcaa gcgtcagaat
     7981 cgcggtgatg gtaaagccag gcgctttgcc aaactgccgc aatgcgtagc ggatgtcctc
     8041 gaaaatggcc tgcatgaaac cttccttacg ttggcagtcg cccgaatgtt ccctgaaatg
     8101 tgcaacacgc cgccagcaac tccaggcgat ttcatgcgat gacactccgc tactgtctgg
     8161 cgaacaatac cgaaggtttt ctcagcatcc ggataaagag tgagtaaaat tgactatatt
     8221 ctggaacaat ccagccgatt aagcacactg taagccgtat ctgcctgtgt atttgaattc
     8281 caccagcttc ttggagcgta gcagtatgaa cagttttctt gccgcccgtg tccttctatg
     8341 cgccgcggcc ctcatccccc tcaacatgtc tggccatgct cagatacaca acaccatgcc
     8401 cgttgtggat gcttcagctc ctccgtctgc agtccgtcag atgccggctt ccgcgctgac
     8461 tccgccccgt ccgttttcaa gattcgcatt cagcggagaa gcctccactc tgggcgtaag
     8521 gctccagact gcggcctacc tggacccgca tctgaacctt cgcgcctccg gaagcattct
     8581 tggattcagc gatacgattt ccaccaacgg ctttcaggtg gatacgaagc tgcatctgag
     8641 ctccgccgga atgtcgctgg attacttccc gtttcccgct cacggctggc gcatcagccc
     8701 tgggctgctg ttttacaacg ccaatggcgc ggacatgacc ttcaccgccc ttcccgggac
     8761 cagcttcagc ctcaataatt acacctacta ttcctcgtcc agcgacccgg tcaaaggcat
     8821 cggcggcgta gggctccata gccataatcc cgcatttacc atcacgaccg gatggggcaa
     8881 tatggtgcgc ccaaaaggcc acttttcttt ccccgtggaa gtcggcgtgg ccctgattgg
     8941 agcgccaaca ttcaacatgg ccctcacttc aggacaggta tgcgacgcca atggtcagaa
     9001 ctgcgtaaac gtggccacag acccggatgt ccaggccaac cttcaggccc aggtaagcaa
     9061 atacagaaaa gacatcgaac cgctgaagac ctatccggtt gtgtctttcg gcgtcgcgta
     9121 taccttccgg cgtcagtatt gatcaccaga cagggggcgg ttcctgtaaa ggaggcagcg
     9181 ggggcaacgg cggctccgcc cttactgcaa ccgcttcttc ttccggaggc tccaggcgat
     9241 tgcccaactc ctgataccgc gacccaaaga agtacgccgc atacgattct ttcacaacag
     9301 ccaccgtccc atagatcgca atcatggccg cgaaataaag cgcaagcaga atcagcgcca
     9361 caatagccgc aagaatgatg aacaccacct gaagcgcgat tcccccatgc cacatgagat
     9421 ggaacaaaag cacgcccccc agcaccaccc ccaaacctgc gatcagcagg aaaatcccaa
     9481 caacaacaag cagcgcccag cttagcccga tcgagagaac aaaacgcagc agcaaatatc
     9541 ccagaaacgt ccccggttcg tctctgagca aactgaaaaa cctgccaaac gcagcttcaa
     9601 tcggagcatc ttcaatcgcc atcggcggca gcacaaagtc ctgcaatacg gtgtccacaa
     9661 tcatccacag caatcccaga agcagaacaa ttcccaaaat cggaaacatt gctgcaaata
     9721 ccggaaaggg attagaggct gccccctgta gcgccagctc tttcatcgtt ctgatcatgc
     9781 gcagaaagag cggccctatg gccaccgccg ccacaatcag aaacgccagc gaagccagca
     9841 gcatgacgcc aaaatatcgc caggactgct gtccgtattt ggaccacgcc tgccgcacct
     9901 tgccctgctt ataaacaatc aggtcaaaca gcgtaaaccg caaacggcag aaaagatacg
     9961 taatccccac ccacacaacc agaccaacca gcaacccaat ggcgaggatg acgagtccgg
    10021 ctaccgtacc gaaaccaggt tgccagagcg tagaaaaatc catcctgtgc tggtgctgtg
    10081 cggcggccgc tccagctgct gcctgcgcgc cctgcattgg ataggaaatc acgaccgaat
    10141 agaaggaggg ctgtgtcagc gcggccacaa gcgcaatctt caggaagaat cctaaacgaa
    10201 acggcttgag catgacctcg cgtgtccggc ggaaggcagg cccgatcgaa tctgttggtg
    10261 acagtatttt catttttcac atgagggtat gccattccgt cgccggctgc aatcaggaaa
    10321 taaatgctct ttcggttcca gcggctcatt ctctttgtgt tcacaaaaat tttcagagaa
    10381 taatgcgcat aacaccctga ccggcccatt gcacatcttg acaaccccac actcgaatca
    10441 atacattgcg actgatgctt tggaaacgat tccaagcatc acaaaagcca ataagtaacg
    10501 tttccatcga aggttgaagt acccacacat tttccccctt aggagggttg tatgttcatt
    10561 cgttggagga cgcatctcac gctcttgctc ctggcagtcc tcggactcgt tccggcactc
    10621 ctggcgcaat ccgactcctc atccatctca ggcaccatca ccgattccgc cggcgccgtc
    10681 gtcccgcgcg ccaccgtcac catccgtaac gaagccactt cagaatcgcg caccgctaca
    10741 tccaatgatg ccggcttcta cactgtttcc agcctgcctc ccggcctgta caccattcag
    10801 atcgaggccc agggatttca gggcttcgtc cagcagcaca cgcaccttga tcccagtatc
    10861 ggtcttcgcg tggatgcggc cctgaaagta ggcagctcat ccacaacggt caatgtcgtt
    10921 gccaatgcca acgtcgtcca gacagaaaca gccgccgtcg gccagttggt cacgcgccag
    10981 caggtacagg ccatccagct caacggacgc aatcccatct accttgctca gatggagcct
    11041 ggcgtgcgcc gcggcaattc catgtcagct ttcggtttcg ggttggacaa tccgttcaac
    11101 atcaatggct ccaactatcg cgaaaacggc cagatctgag acggcgcgcc catggtgcgc
    11161 agccgcggca atggcaccag cgttggtgtt gccgacgtgg attccacctc ggaaatgcag
    11221 gtcctcacag ccgcttatcc ggccgaatat ggccgctcct ccggcggact cattcgcatt
    11281 gtgccccgca gcggaaccag cgaatttcac ggcgcgctct ttgaatacct gcgcaactct
    11341 ttcttcaacg caaatacatg gataagaaaa cagcccgcca acccggcaaa tatcagaaat
    11401 cacccgcagg ccttccgctt taaccagttt ggatggaatt tcagcggtcc tgtctacatc
    11461 cccggcctgc atttcaatga aggacgccac aagctcttct tcctcgctgg acaggaatac
    11521 ctgcgctacc gtcatgacga taccgtcacc cgtaaagtcc cgaccaatct catgcgcacg
    11581 ggaaatttca gcgaattgct ttctcccaat atcttctaca acagcacggt ccagctcgta
    11641 gatccgaata caggccagaa ctacaacggc aacatcatcc cgcccagcga gctaagcccc
    11701 aatggactcg gccttctcaa tgcctatcct gctcccaatg ccgtgaaccc cagctataac
    11761 tggattgacg ctgccatcta ccctgaagac cagcgtaaag acaccctggt ggttgactat
    11821 attcccgccg ataaccacca cctgcgcttc tccctgctga attacaacta cgacagcgtt
    11881 tcgccgcatt acggcaactt taaccgcacc ccgcaggtat ggcaccgccc caaccaggtt
    11941 gccgtcttcc actacacatg gaccatcaat cccacgaccg tgaatgatgc ttttgtttcc
    12001 ggcagcgcag accacgtgcg catcggttac gacctctcca aaggactcta cgaccgcacg
    12061 aaatacggga tcaattaccc ttatctcttc ccggccggcg acaaggaaat ccccaacaag
    12121 atacccacca tcaacatcgc aaactttgac actctggacg gcggtcccta tccttcgcac
    12181 tccggaggcg tcgtttggga catcggtgac agcctcacca aagtcatcgg taaacacacg
    12241 ctgaagttcg gctttcttta tgaacgcgaa agccagaatg acttcgacca gatcaacgta
    12301 agctccacga caccgggcgc tactaacaac cagaatgggt tcttccgctt cactgacacg
    12361 catggaggcg cgcccacatc ccacgccgct gtggccaatg ccgccctcgg attgtttgat
    12421 acctatggcg aaatcggtgt ccgctcctac accgtctacc tgagcaatat gtatgaatac
    12481 tttgctcagg acacctggcg cgctactccc cgcctggtca tcgagtacgg cgtgcgtcac
    12541 agcatcatgc ttccctacta cgccaaatgg ggaaatcagt ccttcttttc tacatccgtc
    12601 tacgacccca caacagcgcc tacgatcgat ccgaccaccg gattcgtctc cggctcgaat
    12661 ctgatggatg ggattgtcat tcccggcaag ggctttcccg gctcggccaa agggcatgtt
    12721 cctgacagca tcctgaacaa cagctactat cagacgttgt ttcatggctt tgatcgtggc
    12781 tataacccgc tcgtctggac caatatccag cctcgcgtgg gaatcactta ctcactcgat
    12841 ccgcaaaccg tcatacgcgc aggcggaggc cgcttctttg accgcctcgg cataagcgac
    12901 accgtccacc tcggcggcaa tccgcccttc cagccctccg gcaccgtcag ctttggctcg
    12961 gtggatgccc cgggtggcgt cggcaccaac aattacccca tcaacatgac gtcttatgcc
    13021 tatcacttcc cttcacccga agcatggtca tggacagtct ctgccgaaca tgaatttcct
    13081 cagatcggta tcttcacgct gggatacgta ggccggcgcg gcatccacct ccagcacttg
    13141 gaaaacatca atcagttgct tcccggcaca ctcataaaca cctcggtaag caaaccagac
    13201 actctccgcc cgtatcaggg ttattcggtc attcagcagc aaaccaacga tggcaggtct
    13261 atctatcact ccttgcaggc tgacctgcgg cggcgcgtca cgcatgggct tatgttcggc
    13321 atcgcctata cgtggtccaa gagtctggac tcgacttcag acgcaggcaa taacattccg
    13381 aactacttcg atcccatgga atactggggc cccagcgatt acgatacccg ccacatcgta
    13441 gtcgttaact atgtctggga cattccttat gccagccatg cctcgaactg ggcagtcaga
    13501 aatgtcttcg gcagatggca gctctccgga acgtcgcagt tccagagcgg ctcacccttg
    13561 tctgtttcca ctgg
//
LOCUS       DASRBV010000065        14148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_307447, whole genome shotgun sequence.
ACCESSION   DASRBV010000065 DASRBV010000000
VERSION     DASRBV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307447"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFA02_02680"
     CDS             complement(<1..182)
                     /locus_tag="VFA02_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08782.1"
                     /translation="MAKIRKLSGTSLSPFLPKPIIHGPLFPPVTLNTLKHPSLRKYAL
                     HLASLLAALKVFSLCTL"
     gene            263..1693
                     /locus_tag="VFA02_02685"
     CDS             263..1693
                     /locus_tag="VFA02_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08783.1"
                     /translation="MQAAAEPGSPAAKTDGVAGKGGSPDLIAILTAMLSPQQGAVQST
                     GTGAATQGKAIDRQIAVIDIDDVVPAQEPLLTETVAQSASGTTTGKSLRSHAQAGKEK
                     YALPVKKEGKEVSFSALAGDAGTNILVPVAVPVPAHELVVHSQAAKSVSQVEMAVSSK
                     PASLSKPESQEKAPALPTMSGAEESGVDASTANPVLTAANSDKNAAMATLPESAVSTP
                     GVVEKTAKPDGIPAQMKPQDAAAGQPVASSSVPVQAFPSTAERTMEEHGQMLKEKSTG
                     KTEGVLSGSSSGKAVHSLQPEQAGVDATGVRMITTMNSIAAHNAPAKNSQDVPQINPF
                     QRMDTGASQATLLRTAPHEIAVGVRDPSLGWIEIQTQAASGHVNASLATSSTEAHTVL
                     TSEVPSLTQFMADRDVPVHSIHVAMQGGDAGSGQPGPGSENARQQAFTYHQSAPVFNT
                     PEAGPSEEESFYQERSGNSSRISVRA"
     gene            1710..2147
                     /locus_tag="VFA02_02690"
     CDS             1710..2147
                     /locus_tag="VFA02_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZU08784.1"
                     /translation="MQAQLTHPAVTALDTPSGSGSTSSSSSTSGSSTEITANDFLTLL
                     VAEMKNQDPTATTDPNAYIDQLVQVNSLEQLIQINQEVGGLATNSNSSTSSNSGSSAD
                     THSVTGRLAGAAHVLANALAPRHENTGAQGVEALSVQNQMQHA"
     gene            2171..3409
                     /locus_tag="VFA02_02695"
     CDS             2171..3409
                     /locus_tag="VFA02_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZU08785.1"
                     /translation="MPSFFIPLSGLEADNTALNTIANNLSNMSTVAYKSQDVQFSDLF
                     YQQVGESGSGNPEQVGAGTKIGAIVTNFSSGTTTPSTGNPQDVALNGDGFFVVQGNGT
                     QEYTRAGNFVLGSDGHLLTQAGQQVMGFPAVNGVVNTNAPLTAIQIPVGQVEQPKTTS
                     NLSVTANLDASAANGTSVQGQVTLYDSLGVGHVATVNFQKTGTNTWTYNISLPAGEAA
                     GSSGNTTGTLTFDTNGNLTAPAANLTNVQFTGLSDGASNLNFTWNLYGSNGKGLIDQV
                     ASASGSGIASMTQDGYEAGQYNGFTVDSSGVISATFSNSQTQVIGQLAVASITNEQGL
                     TRLGDGNYQTTLASGQATIGTAGTGGRGTIQDNAIEGSNVDISTQFADLIVAQRAFEA
                     NAKSVTTFDTVTQDTINMVH"
     gene            complement(4419..5555)
                     /locus_tag="VFA02_02700"
     CDS             complement(4419..5555)
                     /locus_tag="VFA02_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZU08786.1"
                     /translation="MAAAITGCRPHDFPQYPANYREYAYVTNGESNTVTVLDLINLRQ
                     DRVIQVGTNPSGIAANPVRNEVYAVNTGSNSVTVMDAEKNAVVATIPVHSKPYFIDVD
                     TKGERAYVANSGSNNVSVIDLKARREIAAIGVGEAPGLARISPDGDSLVVTNRVSGSV
                     TVIDPHTYKMRSSFSGCPGATDAAILPDSSKAFIACSSGHQVMVIGLARPASAIASEH
                     SDRLLTFLDVGQTPVHLALKPDGGEIFVSNFNSDTISEIATGTNEVGGAYLIGAHPVR
                     SIVSPDNSTLWVSNFNADAIGVYSIDDGKRVNTVPVGGGPDALAFSAEGHLLLAADTR
                     SGDISVVRVLSRTPSGTTRTGSLFTMLPAGRQPNAIVVKAFRLQ"
     gene            complement(5618..6676)
                     /gene="aroF"
                     /locus_tag="VFA02_02705"
     CDS             complement(5618..6676)
                     /gene="aroF"
                     /locus_tag="VFA02_02705"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZU08787.1"
                     /translation="MLVVMKAQATQEEIQAVCNYIQHLGFRPHPLPGAQRTAIGITGN
                     QGQIDQGILEELSGVAEVIQVSKPYKLVSRDAKEEDTIIRFSGTNASIGGRDLAMIAG
                     PCSIESREQAFAIAEAVAKAGAQFFRGGAYKPRTSPYSFQGLGEEALQIMAEVREKFG
                     LLIVSEAIDNEALELVDQYADVIQIGARNMQNFSLLKAAGRKRKPVLLKRGIAATLEE
                     FLMAAEYIMSEGNYQVILCERGVRTFADHTRNTLDLSIVPAVQRLSHLPIIVDPSHGT
                     GKRNKVIPLGRAAIAVGCDGLIIEVHHQPDKALSDGAQSIYPEQFAQLMDEASQIAAV
                     LHRHVPRGMHQPESVAAK"
     gene            complement(6869..7951)
                     /locus_tag="VFA02_02710"
     CDS             complement(6869..7951)
                     /locus_tag="VFA02_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZU08788.1"
                     /translation="MSAPLRHRSYLHEVSWFEWHEFRWTTATRSSIAIAFSLALGLFL
                     GHPGGGMVAAAGAFTAGMGSFQRIGRSCILPMLLASVATPVSTGIGMIMGHQNVWFVF
                     VAGLWALGYGLLAESAGGTSWVGQQCVIVLLVGSAFHVSAGGAIPRCLLMLAGGLLQT
                     GITTAVIYLTPDRPGAFRPKTAPFLDPMLRQQIGGWHRCFTLRSPGCIYAFRQALTVG
                     VSVEIFRRLNVPSGYWIGMTALLVLKRDFRTTLVRGFARLLGTLLGAGIAGVIANFLH
                     LSPVTLCILVVVFAWAAISVTNVNYALYVLNLTAYIAFLLALNGLPPAQVIHRRALCT
                     ALGGVLAILAHLDVLKKAALSFERAG"
     gene            8077..9087
                     /locus_tag="VFA02_02715"
     CDS             8077..9087
                     /locus_tag="VFA02_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HZU08789.1"
                     /translation="MSTKLTPGKLAGLKAVSDERGVIAAAAMDQRGSLKKSLAKEKGA
                     DVPDSALEEFKSLVTEVLTKHASAILLDPEYGLPASRKRNGKGLLLAYEKTGYDAALP
                     GRLPDLLDVWSVRRLKEAGADCIKILLYYTPFEKSSINDHKHAWVERIGDECRAHDIP
                     FFLETVGYDVHGEDEKGLAYAKRKPEIVTGSMEEFGKERYGVDVLKVEVPVQMEFVEG
                     TKAFKGEKAYTRKEALELFRTAASSTHKPFIYLSAGVSNPVFIETLELAAESGTSFNG
                     VLCGRATWKDGIPVYAKQGAKAFEQWLNTEGVKNIENVNNALKAAHSWFDKLGVKAEA
                     LV"
     gene            9198..10706
                     /locus_tag="VFA02_02720"
     CDS             9198..10706
                     /locus_tag="VFA02_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU08790.1"
                     /translation="MESETIQKESVTTRTETFALAGIAAAIALLHLLTNNRYGFHRDE
                     LQFLSDARHLDWGFVAYPPFTPFIERISMGLFGLSLVGLRLFSVLAQAVVVIVAGLMA
                     RELGGGRLAQITAAIAVAFSPLAIFEGTEFQYTSFGYFWWVLISYCFIRLLKDENPRW
                     WLAIGALTGLGLLTKYSIVFYIAGVLGGMLLTRTRRYFLSQWFWGGVAVALLIFLPNL
                     LWQVQHGFISYHFLQSIHKRDVAIGRADGFLFRQFVIGGCALLWVPGLIGFLRSERYR
                     LLAWMYLITLVLFWAGKGRDYYMGEAYPVLVAMGAVMGERWSAALSHTLRWVVRTTFF
                     ALLAGFAAYICAVLVPLASSGPLKDFALRRNGDLREEIGWTELVKTVADIRDSLAPEQ
                     RASVGILVGNYGEQGAIEILGQAYHLPAPISMTNSAWLRGYPEPPPSTLIMVGFSREW
                     VDRNFTSCRLAGHNGNSEGVRNEESQYHPEIFVCGPPRKPWAEFWKENQRFG"
     gene            complement(10703..10954)
                     /locus_tag="VFA02_02725"
     CDS             complement(10703..10954)
                     /locus_tag="VFA02_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08791.1"
                     /translation="MARNLYILAGTLLLFALVSCGMVYVGNSQPGLPGNGSLWKTTAM
                     FLTLGGLIVALAGSLTAMFEQVDRRTEERKYQERKRRHH"
     gene            complement(10957..12954)
                     /locus_tag="VFA02_02730"
     CDS             complement(10957..12954)
                     /locus_tag="VFA02_02730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZU08792.1"
                     /translation="MSTATPQAPTLPSLYDFFAPGGILSRSSLPYEFRKGQLEMAKAV
                     ERALTGQRHLIVEAGTGTGKTLAYLLPALRLGQRVIVSTGTKNLQEQLYFKDVPFLES
                     LLGPLRVCYMKGRNNYLCRHKLYALRDQPILNGLEEIEQYRAIAEWEKTTETGDRAEI
                     ASLPETSAVWSKLDARSDACLGQSCPNYERCFITEMRRRAAESDVVIVNHHLFFADLA
                     IKQQAGGAPDAGVLPDAAAVIFDEAHELEDVASSYFGISLSNLRFEELARDIETMLRA
                     KQALSDGLKSSAEMMRERARMFFGSLPLNMSGEGRVRFDAREEFLETQGDLYIALMNA
                     LHRLEGELERIRGVEETPGLRRRTTDVRAQLEFLLESNDRNTVFWLERRGGGRGRESA
                     RTTYLQATPIDVSDLLSDLLFESFPSVILTSATLTVQGGFVHIRKRLGMRDASELVVP
                     SHFRYGEQALLYLPPEMPDPRDPQFQDEAVRCVRRILEITQGRAFCLFTSYQQMRDIY
                     DRLLMEFSYPLLLQGNAPRKALLEEFRATPNAVLFGTSSFWQGVDVQGEALSCVIIDR
                     LPFAVPTDPVVQARMRSIEENGGMPFFEYQVPSAVITLKQGFGRLVRSLEDRGVLVLL
                     DPRLQKQRYGRIFLESLPPYRITQNIMDVEAFFQQKDAQLR"
     gene            complement(13060..13434)
                     /locus_tag="VFA02_02735"
     CDS             complement(13060..13434)
                     /locus_tag="VFA02_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU08793.1"
                     /translation="MMVLVDTDILIEVSRGRNHDVLTKWKELLESRVLVFYTPVTAAE
                     LWGGIFHHEQKALKALLDGLECLPLDYETGYIAGRYLKQYRKSHGLALGDALIAAAAV
                     QNDAALWTRNRKHFPMKDLVLY"
     gene            complement(13431..13688)
                     /locus_tag="VFA02_02740"
     CDS             complement(13431..13688)
                     /locus_tag="VFA02_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZU08794.1"
                     /translation="MHRTQLYLDDDVWVALRSRAKREGTTISELVRSAVREQYLNKWD
                     KRRKAMEAWIGVAKNRTDLPDATEYVRSLRRDNRWERLEKE"
     gene            13915..>14148
                     /locus_tag="VFA02_02745"
     CDS             13915..>14148
                     /locus_tag="VFA02_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZU08795.1"
                     /translation="MLTLETHVQFVKGIGPQIAQSLAAKGITTVEDLLYHLPFRYEDR
                     LHPRALNELQPGEMASVIAEVRGTALLRTRAMPI"
BASE COUNT         3192 a         3615 c         4167 g         3174 t
ORIGIN      
        1 agtgtgcaaa ggctgaatac cttgagcgcc gcaaggagcg acgccaggtg gagagcgtac
       61 tttctgagcg acggatgttt gagagtgttg agcgtaaccg gcgggaacag cggaccatgg
      121 atgattggtt tgggcagaaa aggcgaaagg gaagtacctg agagttttct tatctttgcc
      181 acattgcttc ggcaaagtat ttgcatcaag aggctacagg agacgaaaga ctgtgcaggt
      241 tagcgcagca ggtttactcg atatgcaagc ggccgcggag ccaggcagcc ctgcagccaa
      301 aacagatggt gtcgcgggga agggtggcag tcctgatctc attgccatac tcacagcgat
      361 gctttcgccg cagcaggggg ccgtgcaaag tacaggtacc ggtgcggcaa cgcagggcaa
      421 ggccatagac aggcaaattg cagtcataga tattgatgat gttgttcctg ctcaggaacc
      481 gctgctaacg gaaactgttg ctcaatctgc atccgggaca acaaccggca agagtctccg
      541 gtctcatgct caggctggca aagagaaata tgcgcttccg gtaaagaaag aagggaaaga
      601 agtctcattc tccgcgctcg ccggagatgc aggtacgaac attctggttc ctgtggctgt
      661 tcctgtccct gcgcacgaac ttgtcgtcca ttcgcaggcg gcaaagagtg tctcgcaggt
      721 tgagatggcg gtatcatcga aacccgcttc attgtcgaag ccagaatcgc aggaaaaggc
      781 acccgcactt ccaacgatga gtggagccga ggaatcaggg gtggacgctt ctacagccaa
      841 ccctgttctc accgcggcga attctgacaa gaacgctgca atggcaactc tgccggaatc
      901 ggcagtatcc acgccgggcg tagtggagaa gaccgcaaaa cccgatggta ttccggcaca
      961 gatgaagccg caagatgctg cggctggtca gcctgtggcc tcctcctctg tgcctgtaca
     1021 agcttttcca tccaccgcag agcggactat ggaagaacac gggcagatgc ttaaggagaa
     1081 atcaacaggg aaaacagaag gagtcttatc aggaagctct tccgggaaag ctgtgcatag
     1141 tctgcagcca gagcaggccg gagtggatgc cactggcgtt cggatgatta caacgatgaa
     1201 ttcaattgcc gcccataatg ctccggcaaa aaacagtcag gatgtgccgc aaataaatcc
     1261 atttcagcgc atggatacgg gagcatcgca ggccactttg ctcaggacgg ccccacatga
     1321 aatcgcggtc ggcgtccgtg atccgtcgct gggttggatt gagatacaga cgcaggccgc
     1381 ttccgggcat gtgaatgcgt cactggccac atcctcgacg gaagcccata cagtgcttac
     1441 gtcggaagta ccttcactga cgcagttcat ggcggaccgg gatgttccgg tccacagcat
     1501 acatgtggcc atgcagggag gcgatgcagg ttcggggcag ccaggtccag gctcggagaa
     1561 tgcccggcag caggcattta cgtatcacca gagcgctcct gttttcaata cgccggaagc
     1621 agggcccagc gaagaagaaa gtttttatca agagagatca ggaaattcgt cacgcatcag
     1681 cgtgcgtgca taaacagaaa ggacatccga tgcaggccca actcactcat ccggcagtca
     1741 cagccctcga taccccgtca ggttccggga gcacatcctc ttcttcttcc acttcgggaa
     1801 gcagtacgga aattacagcc aatgactttc tcacgctgct tgtagcggag atgaaaaacc
     1861 aggatcctac ggccacaact gatccgaatg catatattga ccaactagta caggtgaaca
     1921 gcctggagca gttgatccag attaaccagg aagtaggggg cctcgctacg aattcgaatt
     1981 ccagtacaag ttcaaattca ggttcgagtg ccgatactca ttctgttacc ggtcgtctgg
     2041 cgggcgctgc gcatgttctg gcaaatgcgc tggctccccg tcatgaaaac acaggcgcgc
     2101 aaggagtaga ggcactttcg gttcaaaacc agatgcaaca cgcgtagttc aaacgcacag
     2161 cggaggaaac atgccatctt tttttattcc actctcgggt cttgaggccg ataacacggc
     2221 gctcaatacc attgcaaaca atctttccaa catgagcaca gtggcttaca agagccagga
     2281 tgtgcaattc agcgatcttt tttatcagca ggtaggtgaa agcggctccg gaaatcccga
     2341 gcaggtggga gcaggcacta aaatcggtgc gattgttacc aacttttctt ctggaacgac
     2401 gacaccttcg acagggaacc cgcaggatgt tgccctgaac ggtgacggat tttttgtggt
     2461 ccaggggaat ggaacgcagg aatatacgcg cgccggaaat tttgttcttg gctcagacgg
     2521 acaccttttg acgcaagccg ggcagcaggt gatgggcttt ccggcagtca atggagtagt
     2581 taatacaaat gcgccgctga ctgccattca gattccggtg ggccaggtag agcagccaaa
     2641 gactacctcg aatctctctg tgacggcgaa ccttgatgcc tctgcagcca acggcacatc
     2701 ggtacagggg caggtgacac tctatgattc gcttggcgtg gggcatgtgg ctacggtgaa
     2761 ttttcaaaag accggaacaa acacatggac ttacaatatt tcccttcccg cgggcgaagc
     2821 ggcaggaagc agcggcaaca cgacgggcac tctcacattt gatacaaatg ggaacctcac
     2881 cgctccggca gccaatctga ccaacgtcca gtttactggt ctttcagacg gggccagcaa
     2941 tttgaatttc acctggaacc tgtatggttc caatggcaag gggctgattg atcaggtggc
     3001 gtctgcttcc ggatcaggga ttgcctccat gacgcaggac ggttatgaag ccggtcagta
     3061 caacggtttc acggtggatt ccagtggtgt aatatcggcc actttcagca attcgcagac
     3121 ccaagtcatt ggccagcttg ccgtagccag tattacgaat gaacagggcc ttacgcgcct
     3181 gggtgacggc aattatcaga ctactcttgc ttcggggcag gcgaccatcg ggacggccgg
     3241 caccggaggg cgcgggacga ttcaggacaa cgcgattgaa ggctctaacg ttgatatttc
     3301 gacgcagttt gccgacctga ttgtggcgca gcgtgctttt gaagcgaatg cgaaatctgt
     3361 gactacattc gacacggtaa cgcaggacac cattaacatg gtccactgat tcgtgttctg
     3421 tttttcgatt ttgccagtca tcttatttgc gcgatttcgc gtaataagat gactggcttc
     3481 gggcattcgg gcatttttac agattccatg tcgcttagat ttttcctccc ttccgccgtc
     3541 agaatgacgg acttgtgctt ctacgaaaag tgctaaattc ttgaaaatat ttgagttgtc
     3601 cgcaatagag tgaggccgcg tatcgtaggg ggtacccccc ctgttttgta cgggaatcct
     3661 ttattttcaa taagttgcgg gcagatgtct gtacggagat gctttgtttt caataagtta
     3721 gcaaagatgc ggccagcagc cagcgcctgc ttcgcaggct gtcagccaga gtttcttccc
     3781 agttctccgg cttccataga taaactgggt atccataaag gtcggggcga atatttttgt
     3841 aaattcttgc atctgtgaga gttagtaaag tttaggctct catttttgac gcatttttaa
     3901 agccgtgccc cttcaaaccg aactgccaga tgatttcaac cggatcgttg ggattggcta
     3961 aaccctcgca tcgctgagat ttcttcctcc cttcggtcgt cagaatgacg ggagttatgg
     4021 agtatatagt cggtcgtcag aatgagggac aataagtcca gaatgaccgg actgtaccta
     4081 tacgcggggg aaatgctata gatgcaaatt ttatgtaaat tcttgaaaat atgaggtttt
     4141 tagcgttctc ggccttattg agggcaaaat aatacctaga ttgtctacgt atcccggctc
     4201 gagtcttcgg caaaaattgc ttctttcttt caggatatca ggttgagagg agggaaatgg
     4261 acaatggccg ggttggtaat ctcattgaaa gaaataggct taacggaatt tatgactttt
     4321 gtaagggttg acacgctgtc ttgtcggctg agggggattt ttcgaggccc tctagcttca
     4381 tctgaaagtg cagcgaagcg acgggtatcc cggtaggttc attgcaggcg aaatgctttt
     4441 acgacgatgg cattgggttg tctgccagcc gggagcatgg tgaagagcga tccggtccgg
     4501 gtagttcctg atggtgttcg gctgaggacc cggactacag agatatcgcc ggagcgggtg
     4561 tcggctgcga gcagcagatg gccttctgcg gagaaggcga gtgcgtccgg gcctccgccc
     4621 acggggacag tattgacacg cttgccatca tcaatggaat agacgccaat ggcatcggca
     4681 ttgaagttgc tgacccagag tgtagagttg tccggactaa cgatggagcg cacgggatgc
     4741 gcgccgatga ggtaggcacc gccgacctca tttgtgccag ttgcgatttc agaaatcgtg
     4801 tcgctgttga agttggagac gaagatttct ccgccgtcgg gcttaagggc gagatggacg
     4861 ggtgtctggc ctacatcgag aaaggtgaga agccgatcag agtgctcact ggcgattgcg
     4921 cttgcggggc gggcgagtcc aatgaccatg acctgatgcc cgctggagca ggcgatgaat
     4981 gccttggatg aatcgggcag gatggcagcg tcggttgccc ccgggcatcc gctgaagctg
     5041 ctgcgcattt tgtaggtgtg tgggtctatc accgtaacgc tgccgctcac gcggttggtg
     5101 acgacgaggc tgtctccgtc cggagagatg cgggccagcc cgggcgcttc tcccacgcca
     5161 atggcggcga tttcacgtct ggccttcaaa tcaatgacgg aaacgttgtt tgatccggaa
     5221 ttcgctacat atgcgcgctc gccttttgtg tccacgtcaa tgaagtacgg tttgctgtgg
     5281 acgggaatgg tagcgacgac agcatttttt tccgcatcca tgacggtgac ggagttgctg
     5341 cctgtgttga cggcatagac ctcgttccgc accggatttg ctgcaatccc actggggttt
     5401 gtgcctacct gaatcacccg gtcctgacgg aggttgatca gatcaagaac tgtaacggtg
     5461 ttgctctcgc cgttggtgac gtaggcgtat tcgcgatagt tggcggggta ttggggaaaa
     5521 tcatgggggc ggcagccagt gatggcggct gccaatacca ggagatggac gaaacgcagc
     5581 cgttttccgc ctgaacgaca gatttttcgc atcatgtcta ctttgcagcg acactttcag
     5641 gctgatgcat gccgcgcggc acatgacgat gcaggacggc agcaatctgt gaggcttcgt
     5701 ccatgagctg cgcgaactgc tcgggataaa tggactgcgc gccatctgag agtgctttgt
     5761 ctggctgatg atgcacttcg atgatcaggc catcgcagcc gacggcgata gcggctcgcc
     5821 cgagtgggat gactttgttg cgttttccgg tgccgtggct cgggtccaca atgatgggca
     5881 gatggctcaa ccgctggacg gccggcacaa tgctgaggtc aagggtgttg cgggtgtggt
     5941 cggcaaaggt gcggacgccg cgctcgcaga gaatgacctg gtagttgcct tcgctcatga
     6001 tgtactcggc ggccatgaga aattcttcaa gggtcgctgc gataccgcgt ttcagcagaa
     6061 ccggcttgcg cttgcgtccg gcggccttga gcagtgaaaa gttctgcatg ttgcgcgcgc
     6121 caatctggat gacgtcggca tactggtcta cgagttccaa tgcttcattg tcaatcgctt
     6181 cagaaacgat gagcaggcca aatttttcgc ggacctcggc catgatctga agggcctctt
     6241 cgcccaatcc ctggaaagag tagggagagg tgcgcggctt gtaggcgcct ccgcgaaaga
     6301 actgggcacc ggctttggcg actgcttctg caatggcgaa tgcctgctcg cgcgattcaa
     6361 tggagcaggg gccggcaatc atcgccaagt cacgtcctcc gatcgaggcg ttggtgccgg
     6421 aaaagcggat gatggtgtct tcttccttag cgtcgcggct gacgagtttg tagggtttgg
     6481 aaacctgaat gacttcggcg acgccggaga gttcttcaag gatcccctgg tcaatctggc
     6541 cctgattgcc ggtgatgcca atggcggtgc gctgcgctcc gggcaaagga tgagggcgga
     6601 aacctaaatg ttggatatag ttgcagaccg cctggatttc ttcctgcgtg gcctgcgcct
     6661 tcattacgac aagcatgacg gcgcctttcc tgtttcatcc tcagtgtaat gcaaagcagc
     6721 gcaggaaaag aacttatcgt ttgcgcgaaa ctttctttct gagcgcgtgt ccctacggat
     6781 agaaacggtt gcattcggca acacgttttc catagcagca aaccctcctc ggacaacgag
     6841 gctgctgcga agtggcctcg ttgctttttt atcctgcacg ctcgaatgac agggcagcct
     6901 tcttcagaac atcgaggtgg gccaggatag cgagtactcc gcccagtgcc gtgcacagtg
     6961 ctctgcgatg gatgacctgc gcagggggta gcccattcaa tgccagtaga aatgcaatat
     7021 aggcagtcag gttcaggaca taaagggcgt aattcacatt cgtgacggaa atggccgccc
     7081 aggcaaagac aaccacaaga atgcagagcg tgacagggga aagatgcagg aaattggcga
     7141 tgacacccgc aattccggca cccagaagag tgccaagcag gcgggcaaaa ccacggacca
     7201 gggtggtgcg gaagtcgcgc ttcaatacca gaagggccgt catgccgatc cagtaaccgc
     7261 tagggacatt gaggcggcga aagatttcga cggaaacgcc tactgtcaag gcctggcgga
     7321 aggcataaat acatccggga gagcggagag tgaagcacct atgccagccg ccaatctgct
     7381 ggcgaagcat ggggtcgaga aaaggcgctg tcttgggccg gaatgcgcca ggcctgtcag
     7441 gagtgagata gatgacagcg gttgtgatgc ctgtctgaag caggccaccg gcaagcataa
     7501 gaagacagcg cgggatggcg ccaccggccg atacatggaa tgcgctgccc accaacagca
     7561 caatcacgca ctgctggcct acccagcttg ttccaccggc ggactcggcc aggagaccat
     7621 agcccaaggc ccataagcca gcgacgaaga cgaaccagac attttgatgt cccatgatca
     7681 tgccgattcc ggtagagacc ggtgttgcaa cgctggcgag aagcatgggc aggatgcagg
     7741 agcgcccgat acgctggaac gaacccatgc cagcggtgaa tgctccggcg gctgcaacca
     7801 ttccgccgcc cggatggccg aggaacagac cgagcgccag agagaaggca atggcgatgc
     7861 tggagcgcgt ggcggtcgtc cagcgaaact cgtgccattc aaaccacgag acttcatgta
     7921 gatagctgcg gtgccggagt ggggcgctca tgttgaatcc atcgtaattg gattgaagag
     7981 aaagagcagg ccaggactgc ctcaggttaa aatagtgggt ggaactcttt ttcaaaattc
     8041 ccaaaaccaa ataagagaag agcaggagaa tttacgatgt ccacgaagtt gactcccgga
     8101 aaactggccg gactcaaggc agtctcggac gagcgcggcg taattgccgc agcggctatg
     8161 gaccagcgcg gatcgctgaa gaagtcgctg gcgaaagaga aaggggccga cgttcctgat
     8221 tcggcgcttg aggaattcaa gagtcttgtg acggaagtgc tgacaaagca tgcttcggca
     8281 atccttctcg accctgaata tggacttccg gcttccagga agcggaatgg caaggggctt
     8341 ctgctggcct atgagaagac gggctatgat gcggcacttc cgggccgtct gcctgacctg
     8401 ctcgatgtct ggtcggtgcg ccgcctgaag gaagcgggcg cggattgcat caagatcctg
     8461 ctctattaca cgccttttga aaagtcctcc atcaatgacc ataagcacgc atgggtggag
     8521 cgcataggcg atgagtgccg cgcgcacgat atcccgttct tcctggagac ggttggatac
     8581 gacgtgcatg gagaggacga aaagggcctt gcatacgcaa agcgcaagcc tgagatcgtg
     8641 accggttcga tggaagagtt tggcaaagaa cgctatggcg tggatgtgct gaaggtcgaa
     8701 gtcccggtgc agatggaatt tgttgaggga acgaaggcgt tcaagggtga gaaggcctat
     8761 acgcgcaaag aagcactgga gctcttccgg acggcggctt cttcgaccca caaaccgttc
     8821 atttatctct cggctggtgt ctcgaatcct gtcttcattg agacgctgga actggccgcc
     8881 gaaagcggaa ccagcttcaa cggcgtgctc tgcggccgcg caacctggaa ggatggcatt
     8941 ccggtttacg cgaagcaggg tgccaaggca tttgaacagt ggctcaatac cgaaggcgtg
     9001 aaaaacattg agaacgtgaa caatgcgctg aaagctgcgc attcctggtt tgacaagctg
     9061 ggagtcaagg cagaagcact ggtttagttt tgctttatcc ggactggcga caacgccgca
     9121 gattttcgaa gtctgcggcg ttttttgtct ggaaatggaa ctgccgtcat gcaggctgcg
     9181 taggtcacta tacgactgtg gaaagcgaaa ccattcaaaa agagagtgtt actacccgaa
     9241 cggagacgtt cgcactggct ggaatcgctg ccgcaattgc gctgcttcat ctgcttacga
     9301 acaaccgcta cggatttcat cgcgatgagc ttcagtttct gagcgacgcc aggcatcttg
     9361 attggggatt tgttgcatac cctccattta cgccgtttat cgaacgcatc agcatgggcc
     9421 tgtttggcct ttctcttgtg ggactgcggc tcttttccgt tctggcgcaa gcggtggtgg
     9481 tcatcgttgc aggcttgatg gcgcgggaac tagggggcgg gcgactggcc cagataacag
     9541 cagccatcgc ggtggctttt tcaccactgg cgatttttga aggcacggaa ttccagtaca
     9601 ccagcttcgg gtacttctgg tgggtgctga tctcgtattg tttcatccgg ctgttgaagg
     9661 atgaaaatcc gcgatggtgg ctggcgattg gagcgctgac cggactgggc ctgctgacca
     9721 agtactcgat tgtgttttat atcgcaggcg ttctgggtgg aatgctgctg acgcggacgc
     9781 gacgttattt tctgagtcag tggttctggg gtggggtcgc ggtggccctt ctgatttttc
     9841 tgccgaacct gctgtggcag gtgcagcacg gttttatctc gtatcacttt ctgcaatcca
     9901 ttcataagcg cgatgtagcg ataggcagag cagacggatt tttattccgt cagttcgtca
     9961 tcgggggctg tgcgctgctt tgggtccccg gactgatcgg ttttctgcgc agcgagagat
    10021 accgcctgct ggcatggatg tatctgatca ccctcgtgct tttctgggca gggaagggca
    10081 gggactatta catgggagag gcgtatccgg tgctggtggc catgggcgct gtaatgggcg
    10141 agcgctggag cgcagcgctt tctcacacac tgcggtgggt cgtgaggacg acgttttttg
    10201 ccctgctggc cggttttgcg gcctatatct gcgcggtcct tgtgccgctg gcgtcaagcg
    10261 gaccgctcaa ggattttgcg cttcgtcgca atggcgattt gcgggaggag attggctgga
    10321 ctgagctggt aaaaacagtg gctgatattc gggattcgct cgcaccggag caacgggcca
    10381 gtgtggggat tctcgtaggg aattacggcg agcaaggcgc aattgaaatt cttgggcagg
    10441 cctatcatct gcctgcacct atcagcatga cgaactctgc ctggctccgg ggatatccgg
    10501 agccgcctcc ttccacattg attatggttg gcttctcccg tgaatgggtg gaccggaact
    10561 ttacctcatg cagacttgcc gggcataacg ggaattcgga aggcgtgagg aatgaagaga
    10621 gccagtatca tccggagatt tttgtgtgcg gccctccgcg gaaaccgtgg gcggagttct
    10681 ggaaggagaa ccagcgtttt ggctaatgat gcctgcgctt tcgttcctga tacttacgct
    10741 cttccgtgcg gcggtccacc tgctcaaaca tcgcggtcag cgacccggca agcgcgacaa
    10801 tcaggccgcc gagggtaaga aacattgctg tagtcttcca aagagagcca tttccaggaa
    10861 ggcccggctg cgagttgccc acgtagacca ttccgcagga aacaagcgcg aagagcagca
    10921 aagtaccggc gagaatgtac aggtttcggg ccatttttat cgaagctgtg cgtctttctg
    10981 ctgaaaaaat gcttctacgt ccatgatatt ttgcgtgatg cggtacggtg gcaggctttc
    11041 cagaaaaatc cgtccataac gctgcttctg gagccgcgga tcgagcagaa cgagtacgcc
    11101 gcggtcttcg agagagcgaa ccaggcgccc gaagccctgt ttcagcgtaa tgacggccga
    11161 cggcacctga tattcaaaga atggcattcc gccattctct tcaatggaac gcatccgcgc
    11221 ctgcacgaca ggatcggttg ggacagcaaa gggcagacgg tcaatgatga cacaactgag
    11281 tgcttctccc tgtacgtcaa cgccctgcca gaagctcgat gttccaaaga gtacagcgtt
    11341 gggtgtggcg cgaaattcct ccaataatgc tttgcgcggc gcgttgcctt gcagcagcag
    11401 ggggtaggaa aactccatca gaagacggtc gtagatgtcg cgcatttgct ggtagctggt
    11461 gaagagacag aaggcgcggc cctgcgtgat ttccaatata cggcggacac accggacggc
    11521 ctcatcctga aactgtggat cgcgtgggtc cggcatctcc ggcgggagat agagcaacgc
    11581 ctgttcacca tagcggaagt gcgaaggtac aaccagctcg ctcgcgtctc gcatgcccag
    11641 gcgcttccgg atgtgaacga atccaccctg tacggtgagt gtggccgagg taagaatgac
    11701 gctgggaaaa ctctcaaaaa gcagatcact cagcaggtct gagacatcaa tcggagtggc
    11761 ctggaggtat gtagtgcggg ccgattcgcg gccacgtcct ccgccacgtc gctccagcca
    11821 gaagacggta ttgcggtcgt tggattccag cagaaattcc agttgtgcgc ggacgtcggt
    11881 tgtcctgcgt cgcaggcccg gggtctcctc tactccgcga atgcgctcca gttccccttc
    11941 cagcctgtgc agtgcattca tcaaggcgat gtaaagatcg ccctgagttt ccagaaattc
    12001 ctcgcgggca tcaaagcgga cgcggccttc gccactcatg ttgagaggca gcgagccgaa
    12061 gaacatccgc gcccgttcac gcatcatctc tgccgacgat ttcagcccat cgctcagcgc
    12121 ctgtttggcg cgcagcatgg tttcaatgtc ccgcgccaac tcttcaaaac gaagattgct
    12181 caggctgatg ccgaaatagc tggaggcgac gtcttccagc tcatgcgctt cgtcgaagat
    12241 gaccgcggcg gcatcgggca gaacgcctgc gtcgggtgcg cctccggcct gctgcttgat
    12301 ggcgaggtcg gcgaaaaaga ggtgatggtt tacgatgacg acatcgcttt ctgccgctct
    12361 gcgccgcatt tccgtaataa aacagcgctc gtaattgggg cagctctgac ccaggcaggc
    12421 atcactgcga gcgtcgagct ttgaccagac ggcgctggtt tcgggcaaag aagcaatttc
    12481 ggcgcggtct ccggtttctg tggtcttctc ccactcggca atggcgcgat actgctcgat
    12541 ttcttcaagt ccattcagga tgggctgatc acgtagcgcg taaagtttgt gtcggcacag
    12601 atagttgttc cgtcccttca tgtagcagac gcgcagcggc cccagcagcg attcaagaaa
    12661 gggaacatct ttgaaataga gctgttcctg aagatttttg gtcccggtcg agacaatcac
    12721 ccgctgtccc aagcgcaaag cgggcagcag ataggccaga gtcttgcctg tgcctgtgcc
    12781 cgcttccaca atcaggtggc gttgcccggt cagggcgcgt tcgacggcct tcgccatctc
    12841 caattgccct ttgcggaact catagggaag gctcgatctg gaaagaatgc ccccaggcgc
    12901 aaagaagtca tagagagagg gcagcgtggg agcttgtggg gtagcagttg acacagacga
    12961 ttcgctttat gttctccagt gtagcttatg aggaaggcct tggttttgct ttttcctctt
    13021 cgctaaaccg cgtgacgaaa gatgaggtgc acaggcccat cagtaaagga ccagatcttt
    13081 catgggaaag tgcttccggt ttcttgtcca taatgcggca tcattttgca cggcggccgc
    13141 cgcgatgagg gcatcgccaa gggccaggcc atgggatttg cgatattgtt tgaggtagcg
    13201 tcccgcgatg taccctgttt cgtagtccag gggcaaacat tccaggccat ctaaaagtgc
    13261 tttcagcgct ttttgttcat ggtggaaaat acctccccac aactcagcag cagtaacggg
    13321 agtatagaaa accagaactc ttgattccag caattccttc catttcgtga gcacatcatg
    13381 gtttcgtcct ctggatactt caatcagtat gtcagtatcc accagaacca tcattctttt
    13441 tcaagcctct cccaacggtt gtcgcggcgc aaagaccgga cgtattctgt tgcatcaggc
    13501 aggtcagtcc ggtttttcgc aactccgatc catgcttcca ttgccttcct gcgcttgtcc
    13561 catttattca ggtactgctc acgaacagcc gaacgcacaa gttccgaaat cgttgttcct
    13621 tcgcgcttcg ctcgactgcg taaagcgacc catacatcat catcgaggta tagttgtgtc
    13681 ctgtgcatga tgtatatgat acatcgcgtt caatatcaga taaacaagaa ggggaccgtt
    13741 catattctcc atgcggtgtc ttacgaaatc tgcgtctatt cagggagggg acgtctgtga
    13801 tgtggttttc tactcaacag aaagggcttg agggcaccca ttcaaaagga cccgataggg
    13861 gtggtgcgca atgctcgaac gcctggcccg ccagtccctg ctactatcgc atttgtgctg
    13921 acgcttgaga cccatgtcca gttcgtgaag ggaattggac cgcagattgc gcaatcactt
    13981 gccgccaaag gtattacgac cgttgaggac ctgctgtatc atctgccgtt ccggtatgag
    14041 gacaggctgc atccgcgcgc gttgaacgaa ttacagcccg gcgagatggc cagcgtcatt
    14101 gccgaggtgc gtgggactgc actcctgcgc acacgggcga tgcccatc
//
LOCUS       DASRBV010000066         5707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_459235, whole genome shotgun sequence.
ACCESSION   DASRBV010000066 DASRBV010000000
VERSION     DASRBV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459235"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFA02_02750"
     CDS             <1..673
                     /locus_tag="VFA02_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HZU08796.1"
                     /translation="GNSYFADFTLSLPRQMTFQRSEQLVKDATSAVRRVLPDADVVIH
                     TVPRPSVAESIFDKVRAIAARNNVLLHDVSIQSVDGSLRVEQHIEVAENMPLLEAHQF
                     VRRIEDEIRRELPQVESVLTHIESEPATIEHTEMVTSKRAIEEHLRRAASLLPEIIDI
                     HEVTVGRIGDKIQVSCHCTLPDAMDMQRVHEVITDLEDHFKMECPEVHRVLIHPEPAS
                     DNQHH"
     gene            686..1102
                     /locus_tag="VFA02_02755"
     CDS             686..1102
                     /locus_tag="VFA02_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZU08797.1"
                     /translation="MIASAEWKQEMQFDGRSESGHIIHFDADAAHTAGPSPMEAVLAA
                     LCGCTSVDIVNILEKKREPFTSLTVSAEAKQAEEPPRVFTHIRLVYRIGGNVSRKAVE
                     DAVALSKNKYCSVSKMLEKSAEIEFVIEYADGETRP"
     gene            1099..1860
                     /locus_tag="VFA02_02760"
     CDS             1099..1860
                     /locus_tag="VFA02_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZU08798.1"
                     /translation="MTHFFRMALLVLLLCIVALVSAITTMHFAIHGAEVSVPDFRGMS
                     AADAMRKAAALDLSLSVDNRFYSAELPEGRILTQSPAPGTVVRREWHVRVTESLGPQR
                     IPIPNVIGQQERTATIEMRRAGLDLGSVARMPFAGATEGTVIAQNPAPNAEGVERPSL
                     GLLVSDGVPGDATGFVMPDFTGQMYSSASAAITHAGLKLAPAIDVPASVPAVPAASSA
                     QTPAAPTPPGTVIAQNPPAGHYVDANTPIQFTVVR"
     gene            complement(1861..3222)
                     /gene="rsmB"
                     /locus_tag="VFA02_02765"
     CDS             complement(1861..3222)
                     /gene="rsmB"
                     /locus_tag="VFA02_02765"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZU08799.1"
                     /translation="MKNVAPARAAAFEILSAIERGQGRSDELLHRPAINKLSEADRNL
                     VTNLVMGTLRWQVALDERIAMLLAKPKAQMDVAVRIALRLGAFQLLYLDRIPAYAAIG
                     ESVELAKSAGDRFAAGMVNAVLRKIAAQAKENVTVEVTNVAGLARATAHPLWMIERWT
                     KNFGLDKTKAICEYNQQPAPVFVRLVHAGAEEELLREGIELAPGSFLSNVRCIIHGDI
                     TSTKAFRNGHVRIQDEGSQLVAELAGKGSRVLDACAAPGGKTAILAERNPEAEITACD
                     VSEKRLKEMRRIFRHVPGMAHVKYVTLDATNLNLASEFDLILCDAPCSGTGTLARNPE
                     IRHRLVEKELARQHTRQVRILSSLMRVVKRGGRLLYSTCSLEPEENEAVVQECLRSHP
                     EFRPLPLEGELDRLYSAGILQSGAAEKLTNTALMNGCLRTIPGVHTCDGFFAALLVKT
                     VKT"
     gene            complement(3219..4154)
                     /gene="fmt"
                     /locus_tag="VFA02_02770"
     CDS             complement(3219..4154)
                     /gene="fmt"
                     /locus_tag="VFA02_02770"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZU08800.1"
                     /translation="MRLVFCGTPQFAVPTLKTLLRAGHDIRLVVTQPDRPQGRGMALV
                     APPVKQVALEAGLAIFQPEKIKNNADFRAHLEAIRPEAIIVVAYGRIIPKWMLDLPRY
                     GNLNLHASLLPKYRGAAPVQWAIANGEPVTGATTMRIDEGLDTGDILLQRELPIAPDQ
                     TAEDIFPLLAAMGADLMAETLEGLAAGTIVPKKQDDSKATVAPILKREDGQIDFDRPA
                     MEIYNRWRGFQPWPGVYAQFRGKKLALHRMMPIELRGEEGVPGTVHVDRARFFVDCGS
                     NTRLELIEVQLEGKKRMAAADFLRGHQLKSGEKLG"
     gene            complement(4406..>5707)
                     /locus_tag="VFA02_02775"
     CDS             complement(4406..>5707)
                     /locus_tag="VFA02_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZU08801.1"
                     /translation="HAFTSGIEITWTTQPTHWSNDYFKHLFEYEWELTQSPAGAHQWK
                     PKGDTGNGTVPDAYDSSKRHAPGMLTTDLSLRMDPVYEKISRRFYEHPEEFADAFARA
                     WFKLTHRDMGPRARYLGPEVPAEELIWQDPIPAVNHKLIDEQDIAALKKKILASGLSV
                     SQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEANQPEQLAKVLHTLEEIQGEF
                     NKAQSNGKKVSLADLIVLAGCAGVEQAAKNAGFNVTVPFTPGRMDASQEQTDVESFSV
                     LEPIADGFRNYIKFRNGVPPEFLLVDKAQLLNLTAPEMTVLIGGMRALNTNVGQTKHG
                     VFTKRPETLTNDFFVNLLDMSTEWKPVPDSQDVFEGLDRKTGQRKWTATRVDLVFGAN
                     AHLRALAEVYGAADSQEKFVHDFVRVWDKVMNLDRFDLVQA"
BASE COUNT         1245 a         1614 c         1524 g         1324 t
ORIGIN      
        1 cggcaactca tatttcgcgg acttcacgct ctctctgccg aggcagatga cgttccaacg
       61 ctcggaacaa ctggtaaaag acgcgacctc tgccgtccgc cgtgttttgc cggatgccga
      121 tgtagtcatc catacagtcc cgcgtccttc agtagctgag agcatctttg ataaagtacg
      181 cgccatcgcc gcccgcaata acgtgctgtt gcatgacgtc agcatccagt cagtggatgg
      241 aagtcttcgc gtggaacagc acatcgaagt cgcagaaaat atgccgctgc tggaagcgca
      301 tcaattcgtg cgccggattg aagatgaaat ccggcgcgag ttgccacagg tcgaatccgt
      361 gctcacgcac attgaaagcg agccggcaac gattgaacat acggagatgg tcaccagcaa
      421 acgcgccatt gaagaacacc tgcgccgtgc tgcgtcgttg ctgccagaaa tcattgacat
      481 tcacgaagtc actgttggac ggattggcga taaaattcag gtctcgtgcc actgtactct
      541 gccggatgca atggacatgc agcgtgtaca cgaggtcatt acagacctgg aagaccattt
      601 taagatggag tgccctgaag tccatcgcgt cttaattcat ccggaaccag cttctgacaa
      661 tcaacaccat taagaaagag agcttatgat tgcaagcgca gaatggaagc aagaaatgca
      721 gttcgatggc cgctccgaaa gcggtcacat catccacttt gatgccgacg cagcacacac
      781 ggccgggcca agccctatgg aagccgtgct ggctgctctc tgcggatgca cgtctgtaga
      841 catcgtcaat attcttgaaa aaaagcgcga gccgttcacc agccttactg tttcggctga
      901 agcaaaacag gcagaagagc cgccgcgcgt ctttacgcac atcaggctgg tctatcgcat
      961 tggcggcaat gtttcgcgca aagcagtgga agatgctgtg gcattgtcaa aaaataaata
     1021 ctgctctgtt tccaaaatgc tggaaaagag cgccgaaatc gagtttgtca tcgaatatgc
     1081 cgatggagag acgcgtccat gacacacttc ttccgaatgg cactgctggt cttgcttctc
     1141 tgcatagtgg cgctggtgtc tgccattacg accatgcatt ttgccattca tggagctgaa
     1201 gtctctgttc cggattttcg cgggatgagc gcagccgatg ccatgcggaa ggccgctgcg
     1261 cttgatctaa gcctgagtgt agacaatcgc ttctacagcg cagaattgcc ggaaggacgc
     1321 atacttacgc agtctcctgc gccaggtacg gtggtgcggc gcgaatggca tgtgcgcgtg
     1381 actgagagcc tgggtccgca gcgcattccc attcccaacg taatcgggca gcaggaacga
     1441 actgcaacca ttgaaatgcg ccgcgcaggg cttgatctgg gcagtgtggc gaggatgcca
     1501 tttgccggcg ccacggaagg gactgtcatt gcgcaaaacc ctgccccaaa tgccgaaggc
     1561 gtggagcgcc ccagcctggg cctgctggtg agcgatggcg tccctggtga cgcaaccgga
     1621 tttgttatgc ccgactttac aggccagatg tattcgtctg catcggcggc cattactcat
     1681 gccggactaa aacttgcacc tgcgattgat gttccggcca gtgtgcccgc tgttccggct
     1741 gccagctccg cgcaaacgcc agctgctcca acacctcccg gaactgtgat cgcgcagaat
     1801 ccacccgctg gacattacgt ggatgcaaac acgccgatac agttcaccgt ggttcgctga
     1861 ctatgttttt acagttttta caagcaaggc tgcgaagaaa ccatcgcagg tgtggacgcc
     1921 gggaatcgta cgcagacagc cattcatgag tgcggtattt gtgagttttt ctgcggcccc
     1981 ggattgcaga atgccggcgc tgtacagccg gtcgagttct ccttccagag gaaggggcct
     2041 gaattcagga tggcttctca gacattcctg aacgacagct tcattctctt ccggctcgag
     2101 tgaacacgtg gaatagagca agcgtccacc cctctttaca acccgcatca gagatgacag
     2161 gatcctgacc tggcgcgtgt gctggcgcgc cagttctttt tccaccagac gatgccggat
     2221 ttccggattc cgtgccaggg tccctgtgcc gctgcaggga gcgtcgcaga gaattaagtc
     2281 aaactcagac gccaggttta aatttgtagc atctaaagtt acatatttta catgcgccat
     2341 tccgggaacg tgccggaaga tacggcgcat ttccttgagg cgcttttcgc tgacgtcgca
     2401 tgcagttatc tcagcttctg gattgcgttc agcgagaatg gccgtcttac cgccaggagc
     2461 agcgcaggca tcgaggaccc tgcttccctt gccggcaagt tccgcaacga gctgcgaacc
     2521 ttcatcctga atgcgaacat ggccattacg gaaggccttt gttgaagtga tatcgccatg
     2581 tatgatgcag cggacattcg agagaaagct tcccggcgca agctcgatgc cttcgcgcag
     2641 caattcctct tcagcacctg catgcaccag tctcacaaaa acaggggcag gctgctgatt
     2701 atattcgcag atggcttttg tcttgtctaa gccaaaattt ttggtccatc gctcaatcat
     2761 ccatagcgga tgggccgttg cccgggccaa ccctgccaca tttgtaactt ctacagtgac
     2821 gttttctttt gcctgtgctg caatctttcg caatacggca ttgaccattc ctgcagcgaa
     2881 gcggtctcca gcgctttttg ccagttctac gctttctcca atggccgcat atgccggaat
     2941 gcgatccaga tagagcaact ggaacgcccc taaccgcagg gcgatacgca ccgccacatc
     3001 catctgggcc tttggttttg ccagcaacat tgcaatgcgc tcgtccagag caacttgcca
     3061 tcgcaacgta cccatcacga ggttcgtgac cagattgcgg tcagcttcgg agagtttgtt
     3121 gatagcagga cggtgcagca gttcatcact gcggccctgg ccgcgctcaa tcgctgagag
     3181 aatttcaaag gccgcggcgc gcgctggtgc tacgttcttc atcccagctt ttcgccgctt
     3241 ttcagttggt gaccgcgcaa aaagtctgcg gctgccatgc gctttttgcc ctcaagttgc
     3301 acctctatca actcaagacg cgtgttgctg ccgcaatcta caaagaagcg ggcgcggtct
     3361 acatgtactg ttcccggaac gccttcttcg ccacgaagtt ctatcggcat catgcgatgg
     3421 agagccagtt ttttgccacg aaattgtgca tacacgcctg gccagggctg aaagccgcgc
     3481 cagcggttgt atatctccat ggccggacga tcaaagtcaa tctgcccatc ttcacgcttc
     3541 agaatcggcg cgacggtcgc cttcgaatca tcctgttttt tcggaacgat ggttccggcg
     3601 gcaaggcctt ccagcgtttc ggccatcaga tccgcgccca tcgcggcgag caacggaaaa
     3661 atatcttcgg cagtctgatc aggcgcaatg ggcagttcac gttgcaggag aatgtccccc
     3721 gtgtccagtc cttcatcaat gcgcatggtc gttgcgccgg tcacaggctc accgttggca
     3781 atggcccact ggaccggcgc agctccgcga tattttggca ggagcgatgc atgaaggttc
     3841 agattgccat acctgggcag gtcaagcatc cactttggaa tgatgcggcc ataggcaaca
     3901 acgatgatgg cttccggcct gatggcttca aggtgtgcgc gaaagtcggc attgtttttg
     3961 attttttcag gctggaagat ggcgagtccg gcttcaaggg ccacctgctt taccggagga
     4021 gcaacgagag ccattccccg cccttgtggc cggtcaggtt gcgtaacgac aaggcggatg
     4081 tcgtgtccgg cgcgaagcag cgttttcaga gtagggacag cgaactgagg tgttccgcaa
     4141 aaaacaagac gcatcgtgct gaactctccc tgaatgaatg ttgttctctg aaatttctga
     4201 taacacagca caatcaaaaa cccgccagac gggcgggttg tacacgctgc tttgcttttg
     4261 attctaccag atatggtatt cagctccgca gcctttgggg cccggtgctt tcagccgctg
     4321 gttcccgttc atgatgcgaa agcgaagaaa gaatccccgc aaacatctgc agggattctt
     4381 ttgctgcgct cttttatcgt cgagcctaag cctgcacgag atcgaagcgg tcaaggttca
     4441 tcaccttgtc ccagaccctc acaaaatcat gcacaaactt ctcctgcgaa tctgcggccc
     4501 cgtaaacttc agccaacgcc ctgagatgtg cgtttgcgcc gaagaccagg tccacacgcg
     4561 tggctgtcca tttgcgctga cctgtcttgc ggtcaagccc ttcaaataca tcctgagaat
     4621 ccgggaccgg cttccattcg gtactcatgt ccagcagatt cacaaagaaa tcgttggtca
     4681 acgtttctgg ccgctttgta aaaacgccat gtttcgtttg tcccacgttg gtgttcagag
     4741 cgcgcatgcc gccgatcaaa actgtcatct ccggagcggt cagattcagc aattgcgcct
     4801 tgtctacaag caaaaattca ggaggcacgc cattcctgaa tttgatgtag ttgcggaagc
     4861 cgtcagcgat tggttccagt acagagaagg actccacatc ggtttgttcc tgcgatgcgt
     4921 ccatgcgccc cggagtgaaa ggtaccgtca cgttgaagcc cgcattcttt gccgcctgct
     4981 caacgccggc gcagccagca agaacaatca aatccgcaag agaaaccttt ttaccattcg
     5041 attgcgcctt gttgaattcg ccctggatct cttcaagtgt gtgcagcacc ttcgccaatt
     5101 gctccggctg gttggcttcc cagtctttct gcggggccag ccggatgcgc gcgccattcg
     5161 caccgccgcg cttgtcactg ccgcggaaag tagatgccga tgcccacgcg gtcgagacca
     5221 actgagagac agacaaaccc gatgcaagaa ttttcttctt cagcgcggcg atgtcctgtt
     5281 catcaatcag tttgtggttc accgcgggga tggggtcctg ccagatgagt tcttccgccg
     5341 ggacctccgg gccgagataa cgcgcgcgcg gccccatgtc gcggtgggtc agcttgaacc
     5401 acgcgcgggc gaatgcgtct gcaaactctt ccggatgctc atagaagcgc cgtgaaattt
     5461 tctcgtagac cgggtccatc cgcagcgata aatctgtggt cagcattccc ggtgcgtgcc
     5521 gtttcgagga atcatacgca tctggcaccg taccgttgcc tgtgtccccc tttggtttcc
     5581 actggtgcgc tcccgccgga ctttgcgtca gctcccattc atactcaaac agatgtttga
     5641 aataatcatt gctccagtgt gttggctggg tggtccaggt gatttcaatc ccgctggtga
     5701 atgcatg
//
LOCUS       DASRBV010000067         6862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_509913, whole genome shotgun sequence.
ACCESSION   DASRBV010000067 DASRBV010000000
VERSION     DASRBV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509913"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="accC"
                     /locus_tag="VFA02_02780"
     CDS             complement(<1..1361)
                     /gene="accC"
                     /locus_tag="VFA02_02780"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZU08802.1"
                     /translation="MFRKVLIANRGEIALRVICACKELGIRTVAIYSEADRNSLHVRF
                     ADEAICIGPPRSAESYLNVPAVISAAEIADVDAIHPGYGLLSENANFAEVCRASNIKF
                     IGPPPEVTRLMGEKEKARQAMKKAKVPILPGSDGVIQSEEEALEWAKSVGFPVILKAS
                     AGGGGRGMRVVRSAEELPGLFHAAQTEAANAFGNGDLYMEKFIERPRHIEFQVLADEH
                     GNVMSLGERECSIQRRHQKLIEEAPSLQVNQKLRDDIGKTLKRSLEAVGYQNAGTVEF
                     LMDEDGKLYFIEMNTRIQVEHPVTEMITGIDLVKAQLRIASGEKLSEIVPEPVTIRGH
                     AIECRINAEHPEKFTPSAGKITAFNVPGGNGIRVDTAQYSEGFVPPYYDSLIAKLIAY
                     GKDREEAMARMRRALGMFIVEGIHTTIPLHRGIFEDQDFREGKFDTKFMERYFEREKA
                     RREA"
     gene            complement(1436..1942)
                     /gene="accB"
                     /locus_tag="VFA02_02785"
     CDS             complement(1436..1942)
                     /gene="accB"
                     /locus_tag="VFA02_02785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZU08803.1"
                     /translation="MNADEMKELKELIEFLKENKIGEFDLERGDLKVRIKFAQEGVPA
                     NLAGLAPLLTAAPAVPATLPAVHAAAQVSAPAAAPPAPAESEDASLHIVKSPIVGTFY
                     ESPSPGSPAFAKIGDHVEQGQVLCIIEAMKLMNEIESDASGEVVKRFVENGQPVEYGQ
                     PLFALKPR"
     gene            complement(1972..3060)
                     /locus_tag="VFA02_02790"
     CDS             complement(1972..3060)
                     /locus_tag="VFA02_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZU08804.1"
                     /translation="MDYQKRLRALRGKIQEQKVEALLITHLPDVRYLCGFTGSNAALA
                     VTQQHAVMFTDGRYTAQAKQETRGARVVIAKKSALREACAWLEKSGMKKAYFDPEHTT
                     VFALGMMREAISKRRQQTFFQPLAKPLIAELRMVKDAEELLLMRKAALTGCQVFDAVL
                     PHIEPGVPEVELAATLEFFSRSLGADGMSFETIVASGKRSAFPHGRATAEKLPRKGFV
                     TLDFGVILNGYCSDMTRTVYLGKAGREERSAYEAVLEAQEAAVAAVRPGATCGDVDEA
                     ARQVLRKAGLAKYFTHSTGHGVGIEIHEPPRVAAKQEQVLTQGMVITIEPGIYIEGKF
                     GIRIEDMVAVTESGASILTSAPKAMIQL"
     gene            3180..3779
                     /gene="yihA"
                     /locus_tag="VFA02_02795"
     CDS             3180..3779
                     /gene="yihA"
                     /locus_tag="VFA02_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZU08805.1"
                     /translation="MTRLFAKFLLSAISPEQFPRPTVPEIAFLGRSNVGKSSLLNALL
                     GAKLAHVSSTPGRTRAINFFSITDSPQRQQPQMYFSDLPGYGYAKISKSISAQWPKFI
                     EPYLTERPTLTLCLCLVDSSIPPQARDAQLIAFLKNANRRFLVVGTKADRLSGNGRTK
                     AWTSLREGLAADGLLLCSSKTGAGLKELWAAIYSAIEEK"
     gene            complement(3808..4602)
                     /gene="rsmA"
                     /locus_tag="VFA02_02800"
     CDS             complement(3808..4602)
                     /gene="rsmA"
                     /locus_tag="VFA02_02800"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZU08806.1"
                     /translation="MPQKAKLGQNFLADQTARRRIVEALGDISDTTVVEIGPGKAAIT
                     ELLAQKARELIAVELDRDLAAGLRTRFAAYDHIRIVEQDILTVDLAALRRDKEKLMVV
                     GNLPYYITSDILLHLFRYHEAISRAVVMMQREVADRVVAVPGSRDYGLLSATAQMYAR
                     IERLMTLPPGAFSPPPQVHSSVLRLTFAPRFDELGTDAEGFITFLRKAFAQKRKTLLN
                     NLRSAGYEAAEMTSAAGHAGVSLQARAEALSLEEMAKLYSELAELE"
     gene            4686..6140
                     /gene="glgA"
                     /locus_tag="VFA02_02805"
     CDS             4686..6140
                     /gene="glgA"
                     /locus_tag="VFA02_02805"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZU08807.1"
                     /translation="MHIVFAASECLPFIKTGGLADVIGSLPREIVKRGHQVTVYIPFY
                     RQVRDHFPEKTIAIPSITIPFQYYNRFAAVVDGGKRDGVQFYFIDCPELFDRETPYAT
                     AAGDYPDNWERFGLFCRAVLEASKQLGVPDIFHVHDWQTSLLPVYLRTVYYFDPVLRN
                     VGTVLTIHNAGYHGLFPPVTVERLLLPWDIFTPDKVEFFDSFNFLKGGIVYSDMLTTV
                     SHKYAEEIQTPEFGHGLEGVLQNRSSDLRGILNGVDYAKWDPATDGRIAAHYSMDDLG
                     GKTECKRDLLHAFGAPHLPEDTAVLGIVSRLATQKGFDLTAQIVERLTFENVFLVVLG
                     TGEPYYENLFRTLHERFPEKISVRITYDETLAHKVEAGSDIYLMPSRYEPCGLNQIYS
                     LKYGTVPVVRATGGLDDTIEEWDPEKRTGTGFKFENYYAEDYLRAVQRALATFQDKGA
                     WLTLMRNGMKQDFSWTKPTQEYIEVYKEVARRRS"
     gene            6147..6566
                     /locus_tag="VFA02_02810"
     CDS             6147..6566
                     /locus_tag="VFA02_02810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZU08808.1"
                     /translation="MPQNSTITLRVRYAETDQMGVVYHANYFVWFEMGRVEFLRQLGF
                     EYKQMEVVDDCHLPVIEATCRYKAPARYDEVLALETRLSALRSSVVRFAYRLVRPAPE
                     GPQLLAEAETIHIAVDGKMQKRSLPEKYAAKLRSALT"
BASE COUNT         1481 a         2115 c         1721 g         1545 t
ORIGIN      
        1 gcttcccttc ttgctttctc gcgttcgaag tagcgttcca taaatttcgt atcaaatttg
       61 ccttcgcgga agtcctggtc ttcaaagatt ccgcgatgca gcgggatcgt agtgtgtatt
      121 ccctcgacta taaacatgcc cagcgcgcgg cgcatcctgg ccatcgcctc ttcgcggtcc
      181 ttgccgtagg caatcagctt ggcaatcagc gagtcataat acggcggtac aaagccctcg
      241 gagtattgcg ccgtgtctac gcgaatgccg ttgcctcccg gaacattgaa ggccgtaatc
      301 ttgcccgccg atggcgtaaa tttctccgga tgctcagcat tgatgcggca ttcaattgca
      361 tggccgcgaa tcgttaccgg ttccggcaca atctctgaaa gcttttcgcc agacgcgatg
      421 cgcaactgcg ccttcacaag atcaatgccc gtaatcatct ccgtcaccgg gtgctcgacc
      481 tggatgcgcg tgttcatctc gatgaagtaa agcttgccgt cttcgtccat cagaaactca
      541 acggtcccgg cgttttgata tccaacggct tccagcgacc tcttgagtgt cttgccgata
      601 tcgtcgcgca gtttctggtt tacctggaga gatggggctt cctcaatcag cttttgatgg
      661 cggcgttgaa tcgaacattc acgttcgccg agtgacatga cattgccgtg ctcatcggcc
      721 aacacctgaa attcgatgtg ccgcgggcgt tcaatgaact tctccatgta gagatcgcca
      781 ttgccgaaag cattggcagc ctcggtttga gcagcatgaa agagcccagg caattcttcg
      841 gccgaacgga caacacgcat gccgcgcccg ccgccgcctg ccgatgcctt gagaataacg
      901 ggaaaaccaa cagatttcgc ccactccagc gcctcttcct cgctctggat gacgccatcc
      961 gacccaggaa gaatcggcac cttggccttc ttcattgcct gccgtgcctt ctctttttcg
     1021 cccatcaggc gcgtcacttc cggcggaggc ccaataaact tgatgtttga tgcgcggcag
     1081 acttcggcga agtttgcatt ctcgctcagc aatccatagc cgggatggat cgcgtccaca
     1141 tccgcaattt ccgctgcgct gatgacggcc ggaacgttca gataactttc cgctgagcgc
     1201 ggaggcccga tgcagatcgc ttcatctgca aaacgcacgt gcagcgaatt gcggtcggct
     1261 tcactataaa tggccactgt gcgaatgccc agttctttgc atgcacaaat cacacgcagc
     1321 gcaatttcgc ctcgattggc aataagaact ttccgaaaca tagaagaagt cgtatttttt
     1381 ggttatcgtc cctgaccgga gcgccacagc gcgaagcgga gggacctgca acaatttatc
     1441 gcggcttcag tgcaaacaag ggctgtccgt attccactgg ctggccattt tctacaaagc
     1501 gcttcaccac ttcacccgaa gcgtctgact caatttcgtt catcagcttc atggcttcaa
     1561 taatgcaaag cacctgcccc tgctcgacat ggtcgccaat ctttgcaaac gccggcgaac
     1621 ccggtgatgg cgattcatag aaagtgccta cgatgggcga ttttacgatg tgcagcgaag
     1681 catcttcgct ttccgcaggt gcgggcggag cagcggcagg cgcgctgact tgcgccgcag
     1741 cgtggacggc aggcaaggtt gccggaactg caggagcagc agtcagcaat ggggccaatc
     1801 ctgccagatt tgccggtact ccttcctgcg caaacttgat gcgcactttc aggtcaccgc
     1861 gctccagatc gaactcgcct attttgtttt ctttcaggaa ctcaatcagt tccttcagct
     1921 ctttcatctc gtctgcattc atctttgttt ttctcgcttg ctttgactgc atcatagctg
     1981 gatcatggcc ttcggcgcag acgtcaggat gctcgcgccg ctttccgtca cggccaccat
     2041 gtcttcgatg cgaatgccga atttgccttc aatataaata ccaggttcga ttgtaatcac
     2101 catgccctgc gtcagcacct gctcctgctt tgctgccact cgtggaggct catgaatctc
     2161 gattcctaca ccatgcccgg tcgaatgcgt aaagtacttc gccaaccctg ccttccgcag
     2221 cacctggcgc gcggcttcgt ccacgtcacc acacgttgcc cccggacgca cggcagctac
     2281 tgccgcttcc tgcgcctcaa gcacggcttc atacgctgac cgctcctccc ttcccgcttt
     2341 gccaagatac acggtgcgag tcatgtcaga gcaataacca ttgaggataa caccgaagtc
     2401 caacgtcacg aagcccttgc gcgggagctt ctccgctgtc gcacggccat gcggaaacgc
     2461 tgaacgcttc cccgacgcaa cgattgtctc aaacgacatg ccatccgcgc ccaggctgcg
     2521 tgaaaaaaac tcgagcgtag ctgccagctc cacctctgga acacctggct caatatgcgg
     2581 cagcactgcg tcaaaaacct ggcatccggt caacgctgct tttctcatca gcaacagttc
     2641 ctctgcatct ttcaccatgc gcagctccgc gatcagcggc ttcgccaaag gctgaaaaaa
     2701 agtctgctgt ctgcgtttgc tgatggcttc gcgcatcatg ccgagtgcaa aaacagtggt
     2761 atgctcaggg tcgaaatacg ccttcttcat accggatttc tccagccagg cgcaggcctc
     2821 gcgcagcgca gatttctttg caatcactac ccgggcgccg cgtgtttcct gttttgcctg
     2881 tgcggtatag cgcccatcgg taaacatgac cgcgtgctgc tgcgtgacag cgagtgcggc
     2941 attggatccg gtgaatccgc aaagatagcg cacgtcgggc agatgcgtaa taagaagagc
     3001 ttctaccttc tgttcctgta tctttccacg caacgcgcgc aaacgcttct gataatccat
     3061 cccttcacca agatatcagg tggactgcgc ctccagcggc atgcgcgctt tgcgcgccat
     3121 tcgtgccagc atccctctgg taaccctcga tgcgctgcgc cttttatact ttcctttgca
     3181 tgactcgtct tttcgccaaa ttcctgctct cggccatttc tccagagcaa tttccacggc
     3241 caacggttcc tgaaattgcc tttttaggac gctcgaacgt aggcaaatca agccttttga
     3301 acgcgctgct cggagcaaaa cttgcgcatg tttcgtctac tccgggacgc acacgcgcca
     3361 tcaacttttt ctcgattacc gattcgcctc agcgtcagca gccgcagatg tatttctccg
     3421 atctgccggg atatggctac gcaaagatat cgaagtccat ctcggcacag tggccgaaat
     3481 tcatcgaacc gtatctcacc gaacgtccaa cgctaacgct ctgcctttgt ctcgtggaca
     3541 gcagtattcc tccgcaagcg cgcgatgcgc agcttatcgc ttttcttaag aacgcgaatc
     3601 gccgttttct cgtcgtgggg accaaggctg accggctctc cggaaacggc cggacaaaag
     3661 catggacgtc tctccgcgaa ggactggcag cagatggcct gctgctgtgc tcctcgaaaa
     3721 ccggagcggg gctgaaggaa ctctgggcgg cgatctactc tgccatagag gaaaagtaaa
     3781 tgtcccatat cttgaagcga tgtggaatta ctccagctcc gccagttcgc tatagagctt
     3841 cgccatttcc tcaagcgaaa gcgcctcggc cctcgcctga agcgatactc cggcatggcc
     3901 cgcagcactg gtcatctccg cagcctcata ccccgcagag cgaaggttat tcagcagtgt
     3961 tttgcgtttc tgtgcgaacg cctttcgcag aaaggtaatg aatccctccg catctgttcc
     4021 aagctcgtca aagcgtggag cgaaggtcag ccgtaacacg ctcgaatgca cctgaggagg
     4081 cggtgagaat gcacccggcg gcagtgtcat cagcctctca attcgcgcat acatctgcgc
     4141 cgtcgccgaa agcagcccat aatcgcgtga cccagggact gccacaaccc ggtcggccac
     4201 ctcacgctgc atcatgacga cagcgcgtga aattgcctca tggtaccgga acaggtgcag
     4261 cagaatgtcc gacgtgatgt aatacggcag gttcccaacc accatcagct tctctttgtc
     4321 cctgcgcagg gcagccaaat ctacagtcag gatgtcctgc tcgacaatcc gaatgtggtc
     4381 atacgccgca aatcgcgtgc gcagacccgc cgccaggtcg cgatccagct ccaccgcaat
     4441 cagctctctg gccttctgtg ccagaagctc cgtgatggcg gccttgccag ggccaatctc
     4501 gaccacggtc gtgtccgaga tatcacccag ggcctcaacg atacgcctgc gcgctgtctg
     4561 atcagcaaga aaattctgtc ctaattttgc tttctgcggc atcttatcta agttgctgat
     4621 ggaatgagtt tagacttctt ttgagacctg taaacgatca tacggacaag gagagagccg
     4681 cacacatgca cattgtcttc gctgcgtcag aatgcctgcc ttttatcaag accggaggac
     4741 ttgccgacgt catcggctca ctgccgcgcg aaatcgtcaa acgcggccac caggtcaccg
     4801 tctacatccc cttttatcgc caggtgcgcg accactttcc ggaaaagacc atcgccatcc
     4861 caagcattac catccccttt cagtactaca accgttttgc cgctgttgtg gacggaggca
     4921 agcgcgacgg cgtgcagttt tactttatag attgccccga gctcttcgac cgcgagaccc
     4981 catacgccac ggccgcaggt gattaccctg acaactggga gcgcttcggc ctcttctgcc
     5041 gcgccgtgct ggaagccagc aagcagcttg gcgtgccaga tatcttccac gtgcacgact
     5101 ggcagacctc gctgctgcct gtctatctgc gcacggtcta ttactttgac cccgtcctgc
     5161 gcaatgtcgg gaccgtgctt accatccaca acgccggata tcacggcctg tttccgcctg
     5221 tcaccgtcga gcgcctgctc ttgccgtggg acatcttcac tccggacaaa gtcgagttct
     5281 tcgattcttt taatttcctc aagggcggca tcgtttactc agacatgctg accactgtca
     5341 gccacaaata tgccgaagaa atccagacgc cagaattcgg ccacggactg gaaggcgtcc
     5401 tgcaaaatcg ctcctccgat ctgcggggaa tcctcaacgg cgtggattat gcgaagtggg
     5461 acccggccac tgacggacga atcgccgcgc actactcaat ggatgacctg ggcggaaaga
     5521 ccgagtgcaa gcgcgacctg ctccacgcct ttggcgcgcc ccacctgccg gaggacactg
     5581 ccgtgctcgg tatcgtatcg cgcctggcga cgcagaaggg ttttgacctc accgcgcaaa
     5641 tcgttgaacg tcttaccttc gagaacgtgt ttctggtggt gctgggcaca ggcgagccgt
     5701 attacgaaaa tctcttccgc acgctgcatg agcgtttccc ggaaaaaatc tccgtccgca
     5761 tcacttacga tgaaacgctt gcccacaaag tggaggcagg ctccgacatt tacctcatgc
     5821 cctcacgcta tgaaccctgc ggattgaacc agatttacag cctgaaatac ggcactgttc
     5881 ctgtcgtccg cgccaccgga ggactcgacg ataccattga ggaatgggac ccggaaaagc
     5941 gcaccggcac cggtttcaag ttcgaaaact actatgctga agattacctg agggccgtcc
     6001 agcgcgccct ggccacattt caggacaagg gcgcctggct tactctcatg cgcaacggca
     6061 tgaaacagga cttctcctgg acaaaaccca cccaggaata cattgaagtc tacaaagaag
     6121 tggctcggag gcgcagctag atccagatgc cgcaaaactc caccatcact ctgcgcgtcc
     6181 gttacgctga aacagaccag atgggcgtcg tctaccatgc aaactatttc gtctggtttg
     6241 aaatgggccg cgtcgagttt ctgcgccagt tagggtttga atataagcag atggaagtcg
     6301 tagacgactg ccatctgccg gtcattgagg caacctgccg ctacaaagcg cccgcccgct
     6361 acgatgaagt gctcgcattg gaaacacgcc tctctgccct gcgctccagt gtagtcaggt
     6421 ttgcgtatcg tcttgtccgg cctgcgccgg aaggcccgca actgctcgcc gaggcagaga
     6481 ccatacacat tgccgtagac ggcaaaatgc agaagcggtc cctgccggag aagtatgccg
     6541 caaaacttcg ctcggccctg acctgatttt ttttcgcgca ccggaacgat gcccctccac
     6601 agaagccgtt ccagcaatta cactggttgc gctatagcat tttcacagag ggtctcaacc
     6661 atgccacgaa actacagtcg ccgcgaccaa cagggggccg gcgacactgc acagtgcaaa
     6721 tgcagcgtat tgaatctgtg aaaatgccgt aaaaaatgcc cgcaggaaag gacatctgaa
     6781 tgaagttctc tctcgcccat gcaacgatgc tggccgcagc agcccttctg tttgtcaccg
     6841 gttgcgaaac aggacgcacc ca
//
LOCUS       DASRBV010000068         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_357625, whole genome shotgun sequence.
ACCESSION   DASRBV010000068 DASRBV010000000
VERSION     DASRBV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357625"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFA02_02815"
     CDS             complement(<1..237)
                     /locus_tag="VFA02_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HZU08809.1"
                     /translation="MAITNVNKTSVSTIASPGGTNKNAMAMVTTLFFVWGFLTSLNDI
                     LIPHLKSIFSLNYAEAMLVQFAFFCSYAAFGIPSG"
     gene            435..1556
                     /locus_tag="VFA02_02820"
     CDS             435..1556
                     /locus_tag="VFA02_02820"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HZU08810.1"
                     /translation="MRFARTVFALTISVSAAAVYAQAPSAARLIGTVTSVSGDTVTLK
                     SDAGVLSTVTVSDSTRILRAEPGQKDLSSAAPIHVQDLAVGDRILVRATPGPSANTYT
                     AQTIIAMKKSDIAQKQEQEREQWQHGVGGIVKSIDSAAGTIVVASGVRTITIHATPKT
                     VVRRYAQDSVKFDDARVSTFDQIRPGDQLRARGERTPDGGELTADEIVAGTFRNIAAT
                     VVSVNAGMQSITVMDLATKKPVTIRFTPDSQLHRLDAQVAQIIAMRLKGGARQGAATE
                     QTAGHPMAMNGQPQQHSGDLQQVIQRAPAIQITDLHKGDAVMIVATEGSPDSATAITL
                     LAGVEPMLQASTKESQSLFSSSWSLGGGGEAAAASGQPQ"
     gene            1667..>2277
                     /locus_tag="VFA02_02825"
     CDS             1667..>2277
                     /locus_tag="VFA02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08811.1"
                     /translation="MGLLLTPGAHAQAAKGSLRGQVTDPSGAVIPNAAVSLTTPDGHT
                     VATVTSNGTGAYRVNNLDPGTYIVIVTASGFAPSQSKAVTITAGESKQYDVSLQIQVE
                     QQQVQVQAETTTVDTSPENNANALVIKGKDLDALSDDPDELQNELQALAGPSAGPNGG
                     QIYIDGFTGGQLPPKSAIREIRVNQNPFSAQYDRLGYGRIEIFT"
BASE COUNT          541 a          586 c          653 g          497 t
ORIGIN      
        1 gcctgacgga attccaaagg ccgcatagga gcagaagaag gcaaactgca ccagcatggc
       61 ttcggcataa ttcagggaaa aaatggactt gaggtgtgga atcagaatgt cattcagcga
      121 ggtgagaaag ccccacacaa aaaacaatgt ggtgaccatc gccatggcgt ttttgttggt
      181 gccgcccgga gatgcaatgg tactgacgga tgttttgttt acgtttgtaa tggccattgg
      241 gtgttcggag cctggtcctt tgctgatgat aaatggaatg gagtcatgga gaggaaatca
      301 tatcttaata gaggcttaat ggcgcccgat cagtccttcc ggccttgttt ttctgagact
      361 ttaagacaac ctgttcgtct taccttgtgt agcggtattc ggaatacttt taagtcgtag
      421 aaaagaaaga gttcatgcga ttcgcacgta cagttttcgc gctcacgatt tcggtttcag
      481 cagcagcggt atatgcgcag gcaccttctg ccgcgcggct gattggcaca gtcacatccg
      541 tcagtgggga cacggtcacg ctcaaatcag atgcaggcgt gctttcaacg gtgacggtga
      601 gtgattcgac gcggattctg cgggccgaac ccgggcaaaa agacctaagc agcgcagctc
      661 ccatccatgt gcaggatctg gcggttggag accgcattct ggtgcgggcc acacccggtc
      721 cttctgcaaa tacctacacg gcgcagacca tcatcgcgat gaaaaaaagt gacatcgcgc
      781 agaagcaaga acaggagcgc gaacagtggc agcatggtgt aggtggaatc gtaaaatcta
      841 ttgactccgc agcaggaacc atcgtggtgg cttccggcgt gcgcacaatt accatccacg
      901 cgacccctaa gactgttgtc cggcgctatg cgcaggattc ggtgaagttc gacgatgcaa
      961 gagtttccac gttcgaccag atcaggcccg gagaccagtt acgcgcgcgg ggcgaacgca
     1021 ctccggatgg gggtgaactg acggcggatg aaattgttgc gggcacattc cgcaatattg
     1081 ccgcaacggt tgtaagcgta aatgcgggaa tgcagtccat tactgtaatg gatctggcta
     1141 caaagaagcc tgtgacgatc cgctttactc cggattcgca gttgcacagg cttgatgcgc
     1201 aggtggcaca gataattgcc atgcgtctga aaggcggcgc gcggcaaggc gcagctacgg
     1261 aacaaactgc cggccaccct atggcgatga acgggcagcc tcagcaacat agcggagact
     1321 tgcagcaggt catccagcgg gctcctgcca ttcagattac agatctgcat aaaggcgatg
     1381 cagtgatgat cgttgcaacg gaaggttcgc cggacagtgc cacggccatt actctgcttg
     1441 ccggagtgga acccatgctc caggcttcta ccaaagaaag ccagagcctt ttctcttctt
     1501 cctggagcct cggtggtgga ggcgaagcgg cggccgcgtc cggccaaccg caataaaaac
     1561 tcagggccga ttggcttgat ttcataacgg ccacaatgca cgactgaact aaatttttgg
     1621 agactttgtg aaacggagag ttctgctatc ctgcttttct gtccttttgg gtttactgct
     1681 gaccccaggc gcccatgcgc aggcggcgaa aggatcgctg cgcggccagg tgactgaccc
     1741 gtcgggtgct gtcatcccga atgctgctgt cagccttacc acgcctgatg gccacacggt
     1801 tgccacggtc acttccaacg gcactggcgc gtatcgcgtg aacaaccttg atcctggtac
     1861 ttatattgtg attgtcacgg cgtcgggatt tgcgccatcg caatcgaagg ccgttacgat
     1921 tacggcgggc gagtcgaagc agtatgacgt ttctctgcag attcaggtag agcagcagca
     1981 ggtacaggtc caggcagaga cgaccactgt agataccagt ccggaaaaca atgccaatgc
     2041 tcttgtgatc aagggcaaag acctggatgc cctctctgat gatccggacg aattgcagaa
     2101 tgaacttcag gcactggccg ggccatctgc agggccgaac ggcggccaga tttacattga
     2161 cggttttacc ggaggacagc tcccgcccaa gtctgcgatt cgtgaaatcc gcgtcaacca
     2221 gaacccgttt tccgcacaat atgaccggct gggttatggg cgtattgaaa tctttac
//
LOCUS       DASRBV010000069        17813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_715184, whole genome shotgun sequence.
ACCESSION   DASRBV010000069 DASRBV010000000
VERSION     DASRBV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 17813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17813
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715184"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFA02_02830"
     CDS             <1..445
                     /locus_tag="VFA02_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU08812.1"
                     /translation="VLLVVTDGEDNASSLNLEQTIRRVQDLQGPVVYSIGLLFGDSGG
                     GREARRAKRALQLLSSETGGTAYFPRSLSEVDQICAEVARDIRNQYTIGYHSTKPPSL
                     GGYRMVKVVASAPKHGKLVVRTRTGYYPKTDKSKTASATTQPSTQ"
     gene            complement(579..4358)
                     /locus_tag="VFA02_02835"
     CDS             complement(579..4358)
                     /locus_tag="VFA02_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08813.1"
                     /translation="MNRRIAAAAILAFAVFFFVFHTVNAQQATTSLSGVVTDSSGALV
                     PNASVTITRESTGQTLSTTTNSHGEYQFQQLAPGTWTVKVSASGFGSQSKVGNLLVSK
                     PASIDFQMGLESVSQTVNVSAETETLNTSDASVGNAYSNQTIQQLPIIDRNVPDLLSL
                     QPGVLYLGNQVGGPSDMNDSRTGAVNGIRSDQGNVTIDGVDNNDQKMGYAFTGVLRET
                     LDSVDEFRVTTAMSNSDQGRAAGAQINLVTKRGTDQFHGGVYEYNRNTATAANDWFNK
                     QAELASGLPNKPGSYIRNTYGFDVGGPILKQKLFFFVNYEASRIRENYQITQETPTDS
                     LKQGNIYYQSNGNNVELNQAQIAVMDPSCSANGTCPWGPGVDPNVLKLLQSYPTANGA
                     ALGDGLNLGSFSFSSPNPVNLNTTIVRLDWAPTSHNQLFFRGNLQDDTTLDTQQFPGQ
                     PPSHRIRDNTKGLAAGHTWTISNTLVNDFRYGYTRQGRSQAGLDCGSYVQTLRTVSSP
                     TAQSCTTIFNVPVHNVIDNASWAHGNHTLSFGVNWRGITNYSQTNANSYGFASMNIQW
                     LNTGGSIAGTGGSLDPAAFGYPAVDSGDQTTYNEEIGLLTGLVPFIQGQYNFKVGKGG
                     QSGTTIPEGAPISLTYHSNELEYYIQDQWKPQPNVTFTFGIRQVFLQPPYEVHGQQVQ
                     PTVDTHQWFVNRYTQMLRGVADQPDLSFAPSGKVNGRSSYWNMSWKNIAPRVSMVFAP
                     DNKTSVRVGGGVFFDHFGQGIVDTFAQYGSFGLQTQISNPAGQYSVDNSPRFTSLTSI
                     PPLQGVNIPSVIQYPYTPPNNVNTGMAITWGVDNRIKTPYAVTADFSIQRELPHGFSL
                     EANYIGTFGRHLLQQLDLAEPLDLVDPKSGMDYFQAGTLLAKATYAGQTNVAPIPYWE
                     DMFPWLATNGMSATQNIYTNQYQPLAAVGNDSLALANLDAFCASNLGCGPYQDASGNV
                     TTRFYQRQFSSLFAWSSIGSSDYHAMQLTLHRVSSSGLAFDFSYALAKSIDMGSDTER
                     ASEFTSSGGSFSYITNSFNPKGLRAVSDFDVRHLVTGNFIYPLPFGNGRQFAAGVNRA
                     VDAVIGGWTLAGIVRWSSGLPFSILPPLSYATNYQQNAFAVVTGPVKIHKHLVNGLPE
                     VFADPDALNNGIATGSPLRYPYPGEQGTRNAFRGDGYFEPDASLAKTWKTFHEQTLKF
                     AWEVFNVSNSARFDTSPISSRGGLNIQVTSGAGFGIYSSTLVQSRKQQFSLRYDF"
     gene            complement(4507..5367)
                     /locus_tag="VFA02_02840"
     CDS             complement(4507..5367)
                     /locus_tag="VFA02_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08814.1"
                     /translation="MAFFLVPFFVGARDPLYAQTDLPDAPSASITSSPDAGQSFPPNA
                     PQPPKSAFSPRSPFPSQQPACAQTQSKQTSPPGMSCGPAFDPFQRFLNSAEAHPMTPR
                     QKAVLAGKDVIDPFNLLTIGALSAISIGADSHSAYGPGMKGWAKLSGVSFTQDMTNEF
                     FGTFLVPSIAHQDPHYHRMPNASYKRRIAHCIYQVVWTQSDTGKGMFNYAAVIGGAIE
                     EGISDAYVPYRETGWAAASSRYGVALATDPIGNFITEFVPDLARHVNLRVVFVQRIIN
                     QVAKQEGQGQ"
     gene            complement(5455..6069)
                     /locus_tag="VFA02_02845"
     CDS             complement(5455..6069)
                     /locus_tag="VFA02_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08815.1"
                     /translation="MPDAPQNNTPQAVAKRKWSDIVEPGEKVPPLYSKDKLMFPIHEE
                     LRLTGWFAPFLSAGWGQLVDDHPKYGTDSGAFGQRFGAAAIRDLGMRAFSDGILPALL
                     REDPRYFRKRDGSIAHRGLYAASRVFVTQRDNGSSGFNNSVVIGHGIASALTLAYYPD
                     ESATASVVFETWGISLLGEAAGNLWSEFWPDVRQKIFHHHHPQP"
     gene            6333..6545
                     /locus_tag="VFA02_02850"
     CDS             6333..6545
                     /locus_tag="VFA02_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08816.1"
                     /translation="MAENKFEELGRKVEQKMNTVTPRIEEEIKKMIAYLNDEVVPKVR
                     QNSSETMRAAAQQLRKLADYLDRRQP"
     gene            6545..7315
                     /locus_tag="VFA02_02855"
     CDS             6545..7315
                     /locus_tag="VFA02_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZU08817.1"
                     /translation="MAYRAKFSFVAIAATVAIAGCSHKQSAVYVPPPPPSVAPAPSSP
                     ATTANVPPPSLGPGPAAADSTADADFVATHRPIYSETGMASWYGPPYHNHRGANGQIY
                     DQNALSAAHRTLPMGSLIRVTNLRTGQSAVMRVTDRGPFVQDRVIDLSVASAKAVGVW
                     RPGTAEVRIDVYAAPKPIDDGGRWCVQIGAFSKEKDAVKLEEHLEHKYHTANVIEFTG
                     PTGHWVRIRPLNDDKKRAMEIAKEIRPEEGGAYLVRLD"
     gene            complement(7411..8691)
                     /locus_tag="VFA02_02860"
     CDS             complement(7411..8691)
                     /locus_tag="VFA02_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU08818.1"
                     /translation="MMSSVAVAATDAHRAVEAVWRIESAKLIAGLARITRDIGLAEDL
                     AQEALVSALEQWPASGIPENPAAWLMTTAKRRAIDLFRRNAMLERKHEEIGRELEEKQ
                     MNAQPGFDAMDGEIGDDMLRLIFTACHPVLSTEARVALTLRMIGGLTTPEIARSFLIP
                     EATLAQRIVRAKKTLAEAHVPFETPRGAELTTRLSSVLSVIYLIFNEGHTATAGEAWT
                     RPSLCEEALRLGRMVAGLLPGEPEVQALIALMEIQASRLPARTAPDGSPVLLLDQDRS
                     RWDQLLVHRGLAALERAQALGGPRGPYLLQAEIAACHARALRPEDTDWNRIAGLYAEL
                     AQIMPSPVVELNRAVAVGMASGPAAGLALTEALVNHPSLKNYHLLPSVRGDFLYKLGR
                     LAEARDEFERAANMTRNERERRLLLDRAKKCMQG"
     gene            complement(8724..9077)
                     /locus_tag="VFA02_02865"
     CDS             complement(8724..9077)
                     /locus_tag="VFA02_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZU08819.1"
                     /translation="MRFVILRKSDATMESCAPVTPELIEAMRRYDDEMRKAGVLIAVE
                     RLQPTAKGARVHLSGGKYTVTDGPFSETKELIAGVTMIEVASREEAIAWIKRCPTLQS
                     GNIEAEFEIREVLDC"
     gene            complement(9097..9459)
                     /locus_tag="VFA02_02870"
     CDS             complement(9097..9459)
                     /locus_tag="VFA02_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU08820.1"
                     /translation="MTGRILSTLAILFLLMDGTMKLFRPAPVLKAQAELGWPGHLTVA
                     TGIVLLICTLLYAVPRTAVFGAILLTGYLGGAVAVQLRVGNPLFAETLFPVYFGILLW
                     AGIYLREPRLRAIIPLRS"
     gene            9605..10387
                     /locus_tag="VFA02_02875"
     CDS             9605..10387
                     /locus_tag="VFA02_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU08821.1"
                     /translation="MSYENILVEMRGAAALVTLNRPKVLNALNVATVRELDAAIAELA
                     GHKEVRAILLTGAGEKAFAAGADIQELALLPAVEGQELALRGQQVFDRIENCGKPVIA
                     CVNGFALGGGCELAMACTLRIASETAKFGQPEVKLGLIPGYGGSQRLPRLIGKGAALR
                     LLLTGEIISAAEALRLGLVEEVVAPDQLLARAEQIAQAIAAVAPVAVQKCMEAVHAGY
                     DLPLRQALDLEASLFGLCCATEDKAEGTKAFLEKRAAVWKGK"
     gene            complement(10508..11926)
                     /gene="dnaA"
                     /locus_tag="VFA02_02880"
     CDS             complement(10508..11926)
                     /gene="dnaA"
                     /locus_tag="VFA02_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZU08822.1"
                     /translation="MSFVATAPATLNPWVRILGALEKKINRQSFDTWLKPTRFSHING
                     KKLYVRIPTPEFQHVGDRYGDLIQEAVDNLQMEFDEVIFVTPEEDPTLVRVREDGGFA
                     PVPAHSPAAPQSAQRSVSAPGPLQKKFDWSTAAQLNPRYTFDAFVIGSGNQFARAAAE
                     AVAERPSKAYNPLFLYGGVGMGKTHLMHAIGHEVKRRDPQAAICYVTSEKFTNDTINS
                     LRYDRMTSFRDKYRSVDLLLIDDIQFLAQKERTQEEFFHTFNTLHENMKQIVIASDRP
                     PKELAEIEDRLRSRFEWGLIADIQPPDLETKVAILQKKAENDHVTLPTDVAFFIASNV
                     RTNVRELEGALTRLFAWCSMSGVEITLPAAQQCLKQFIDTQVRKITIEAIQRAVAEQF
                     GMRVAELKQKNNSRSVVVPRQIAMFLAKSMTEASLPEIGRQFGGKHHTTVMHSIAKIE
                     EQRRADKDLNRMINKLQEMLNN"
     gene            complement(12317..14317)
                     /locus_tag="VFA02_02885"
     CDS             complement(12317..14317)
                     /locus_tag="VFA02_02885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZU08823.1"
                     /translation="MFAQNASEKKLHALDPTLMDTSVDPCVNFYQYSCGGWLRQNPIP
                     ADESSYGRDTELANQNRLVLKSILERAAAGGTARTPNEQKIGDFYATCTDTSVINQAG
                     LKPLQPILDRIAALKSKNDLPELAAYLDSIGISSFFGFGSDQDFKDATQEIAEFDQAE
                     LGLPEKGYYERTDDKSVKLREQYKAHVARTFELIGEPQAQAAKDAEAVLKLETELAKH
                     SLTNVERRDPQALYHKMTLEKFEGSTPDFAFARYLRALDVPPVQSLNVTEPGFFTGLN
                     EVLASASLDSIKTYLRWAAIRQVPGTALPEALDEESFNFYGKILEGQQQQQPRWKRCV
                     RATDHALGEALGQVYVSERFSPQDKQRTLELTRDIESAMGRDIDQLDWMSAETKLQAK
                     AKLHAVANKIGYPDKWRDYSSLTIVRGDALGNAQRAAAFEVRRQINKIGKPVDRGEWG
                     MTPPTVNAYYNPLMNDINFPAGILQPPYFDSSQNDAVNYGDAGGVIGHELTHGFDDQG
                     RQFDAQGNLKDWWTPEDEKKFNQRADCIVKEYDGFVAVDDLHVNGRLTLGENIADLGG
                     LKLAFLAYLDRAQKDGTNLEKKAGPEYANLTPEQQFFVSYGQGWCQNNRPENLRLRVQ
                     TDPHSPEEFRVNGVVVNLPEFQKAFGCKTGSPMAPMSRCSIW"
     gene            14525..14680
                     /gene="rpmH"
                     /locus_tag="VFA02_02890"
     CDS             14525..14680
                     /gene="rpmH"
                     /locus_tag="VFA02_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZU08824.1"
                     /translation="MPKRTFQPNRRRRSKTHGFRTRMKTKSGAAVLSRRRAKGRHRVA
                     VSAGHRD"
     gene            14738..15136
                     /gene="rnpA"
                     /locus_tag="VFA02_02895"
     CDS             14738..15136
                     /gene="rnpA"
                     /locus_tag="VFA02_02895"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZU08825.1"
                     /translation="MQEQASERTPRTLRGARLSRHSDYQRVYQQSRKYFSASMTYFFY
                     RREDGLHSGPRIGLTAGRVLGKAVERNRIKRRMREAARLNLEHLRADVDVVLHPRRSV
                     LEMDFSRLRDEVGEIFAKIEAAVKQKEKSA"
     gene            15133..15411
                     /gene="yidD"
                     /locus_tag="VFA02_02900"
     CDS             15133..15411
                     /gene="yidD"
                     /locus_tag="VFA02_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZU08826.1"
                     /translation="MNSSRRSIVSPVAAVHVIYKFVLSPLLHLGAGMSGGACRFQPTC
                     SEYANIAIHEYGLLRGGWMALKRFSRCRPGCAGGYDPVPYTDRQSQLP"
     gene            15445..17196
                     /gene="yidC"
                     /locus_tag="VFA02_02905"
     CDS             15445..17196
                     /gene="yidC"
                     /locus_tag="VFA02_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZU08827.1"
                     /translation="MAEIQNPNQQGGGQDSKTLLTFTLVFFVLFLGLQFFMKPKKPET
                     PAQNQPQQQQQQKQEAADNPAASAAAPVNVSSGPVIQAEAESTTVVENDLYRITFTNR
                     GAQVTSWVLKKYKDDDGKPLDLVNHAAAAKYGYPLSLYTYDAKLRSQLQQALFVPSAT
                     GSVKAPGTLSFEYSSGGLSVHKTFRFDGSYVIHADVSVTQNGTPVAAMLAWPSGFGDQ
                     NTLPDYAKATVVTAQSGKADQIPAKKVSGGETLQGPFDWAGVSDLYFGAFFLPENPKD
                     LSLVSLHDTIAIPQNRKNPDPGRTEKASVLGAAMGAAQPYSVRLFAGPKELDVLASVP
                     TSGVGLQKAVSFGMWGPISKPLFLVLRFLYEHVVPNWGWAILILTVILNLAMLPTRFQ
                     MMKSSLKMQRIQPQIEAIKAKYAKYKATDPRRQEMNKEVFELQRREGVNMFGGCIPML
                     IQWPLLYAFYRMLENVIELRQAHWLWLPDLSAPDPLHILPIFFIVSMFLVQWLTPSPG
                     VDPSQQRMMALTMPAVFGFMTWNVGSGLALYWAGSNILGIVQQMLMNRTKLGREMRAL
                     ALKRAAKRAGKPALARR"
     gene            17284..17811
                     /locus_tag="VFA02_02910"
     CDS             17284..17811
                     /locus_tag="VFA02_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZU08828.1"
                     /translation="MPIDDYSAAAQKIAAFLKNLTALGGLRLKYRITAGAGAADPEGL
                     EAREIYVELAGPDAYLLTQRGGELLRALEHVAAKIVRLEADEHDKISFDANNFKTLRA
                     RELKMAAETAADKVRRTGQPYSFAPMSSRERRMLHLAFRSYDDVETASSGEGLRRYVV
                     AYPKGHAQAKAQMRR"
BASE COUNT         3725 a         4682 c         5465 g         3941 t
ORIGIN      
        1 ggtgctgctg gtggtcaccg atggagagga caatgcgtcg agcctgaatc tggaacagac
       61 gatccgccgc gtgcaggacc tgcaaggccc ggtcgtctat tcgatagggc tactgtttgg
      121 ggattcaggc gggggacgcg aagcgcgccg cgccaaacgc gcactgcaac tgctttcgtc
      181 tgaaaccgga gggactgcgt attttccgcg ttcgctttca gaagtagacc agatctgcgc
      241 cgaggtggcc cgcgatatcc gcaatcagta cacgattggg tatcattcga ccaagccgcc
      301 cagtctgggc ggataccgca tggtgaaggt cgtggcgagc gctccgaagc atggcaagct
      361 ggtggtgcgc acccgcacgg ggtattaccc gaagacggac aagagcaaga ccgcgtctgc
      421 aaccacccaa ccatccacgc agtgagtccg gtcattccgg gtggtacggc aatcttagta
      481 gtattggcta aaccttacat cgccagaatt cttcttctct ttggccgtca gaatgacgga
      541 cgtgtaggaa aaacgctata aggcactgcg taggcgcctt aaaaatcata gcggagagag
      601 aactgttgtt ttctcgactg cactaatgtc gagctgtaga taccaaaccc ggcgccgctg
      661 gtgacttgta tattcaggcc tccgcggctg ctgatggggc tggtatcaaa gcgggcggaa
      721 ttcgatacgt tgaacacttc ccaggcgaac ttgagcgtct gttcgtggaa ggtcttccag
      781 gtctttgcga ggctggcgtc cggctcgaag tagccatcgc cgcggaatgc gttgcgggta
      841 ccttgctctc ccgggtaggg ataccggagc gggctgccgg tggcaatgcc gttgttcaga
      901 gcgtcgggat cggcgaagac ctccggcagg ccattgacca gatgcttgtg aattttgacc
      961 ggtcctgtta ccacggcgaa cgcattctgc tggtagttgg ttgcatagct gagtggaggc
     1021 aggatgctga aaggcaagcc actggaccag cggacgatgc ccgcgagtgt ccagccgccg
     1081 atgacagcat ctacggcgcg gtttacgcct gcggcgaact gcctgccatt gccgaaggga
     1141 agcggataga taaagtttcc agtgacaaga tggcgcacat cgaagtcaga gacggcacga
     1201 agtcctttgg ggttgaagct gttggtgata tagctgaaac tgccgccgga agaggtaaac
     1261 tcgctggccc gttccgtatc ggaacccatg tcaatggatt ttgccagagc ataactgaaa
     1321 tcgaaagcaa ggcctgaact gctgacccga tgcaaggtga gttgcatggc gtggtaatca
     1381 ctactgccga tggaagacca ggcaaacaac gacgaaaact ggcgctgata gaagcgcgta
     1441 gtgacattgc cgctggcgtc ctgatagggg ccacagccaa gattgctggc gcagaatgca
     1501 tccaggttgg caagggcaag agaatcgttt ccgacggcag ccaggggctg gtattggttc
     1561 gtgtagatgt tctgggtggc gctcattcca ttggttgcca gccagggaaa catgtcttcc
     1621 cagtaaggga tgggcgcaac attggtctgg ccggcatagg tggctttggc caaaagcgtg
     1681 cctgcctgga aataatccat tccggacttt gggtctacga gatccagcgg ttcggccagg
     1741 tcgagctgct gcaacaaatg ccgtccgaaa gtgccgatgt agttggcttc cagagaaaat
     1801 ccatggggca actctctctg aatggagaaa tctgctgtaa cggcgtaggg tgttttgatt
     1861 ctgttgtcta cgccccaggt gatcgccatg ccggtattga cgttgttggg tggagtgtag
     1921 ggatattgaa tgacggacgg gatgttgacg ccctgtaatg gcggaatcga agtcaggctg
     1981 gtaaagcgtg gcgaattatc tacactgtac tggccggcgg ggttgctgat ctgtgtctga
     2041 agcccgaagg agccgtactg ggcgaaggtg tccacgatcc cctgaccgaa gtggtcgaaa
     2101 aacactccac cgccgacgcg gaccgacgtc ttattgtcgg gcgcaaagac catagagacg
     2161 cgtggagcaa tgtttttcca ggacatgttc cagtacgaag acctgccgtt tactttgccg
     2221 gaaggggcaa aggacaaatc gggctggtca gccacaccgc gaagcatttg ggtgtagcgg
     2281 ttgacgaacc attggtgagt gtccacagta ggctggacct gctggccgtg aacttcatag
     2341 gggggctgaa gaaatacctg gcggattccg aaagtgaagg tgacgttcgg ctgtggtttc
     2401 cactggtcct gaatatagta ttcaagctcg ttggaatggt aggtaaggct gatgggggcc
     2461 ccttcaggaa tcgtggtccc gctctggcct cctttgccga ctttgaagtt gtattgcccc
     2521 tggataaatg ggaccagccc agtgagcaga ccgatttctt cgttataagt agtctggtcg
     2581 cctgaatcta cggcgggata gccgaatgcg gcagggtcca ggctgcctcc tgtgcctgcg
     2641 atggaacctc cggtgttcag ccactggatg ttcatgctgg cgaagccata ggagtttgca
     2701 tttgtctgtg agtagttcgt gatgccgcgc cagttcacgc cgaaggacag agtgtggttt
     2761 ccgtgcgccc aactcgcatt atcaattacg ttgtgcacag gcacattgaa aatggtggtg
     2821 cagctttgcg ccgtggggga tgagactgtg cgcagggtct ggacataaga gccacaatcc
     2881 agaccggcct gacttctgcc ttgccgcgtg tagccgtagc ggaagtcatt gacgagggtg
     2941 ttggagatag tccaagtgtg gcctgctgcg agtcccttgg tgttgtctcg gattctgtgc
     3001 gagggaggtt gtccggggaa ctgctgtgta tccagggttg tatcgtcctg aaggtttccc
     3061 cggaagaaca actgattgtg cgatgtgggc gcccagtcaa gccggacgat cgtagtgttc
     3121 aggttgacag ggtttggcga ggaaaagctg aacgagccga ggttcaggcc atcgcctaac
     3181 gcggccccat tcgccgttgg atagctctgc agcagtttga gaacattggg atccacgccc
     3241 ggtccccagg gacaggtgcc gtttgcggaa cagctggggt ccatgacggc gatctgggcc
     3301 tgattcaatt cgacattgtt tccgttgctc tggtaataaa tattgccttg cttcaggctg
     3361 tcggtgggcg tctcctgcgt aatctgataa ttctcgcgta tgcgcgaggc ctcataattt
     3421 acgaagaaaa acagcttttg tttcaaaatg gggccgccga cgtcgaatcc ataggtattg
     3481 cggatatagc tgcctggttt gttgggcagg ccgctggcga gttcggcctg tttgttgaac
     3541 cagtcgttcg cggcagtagc ggtattgcgg ttgtattcgt acacgcctcc gtggaactgg
     3601 tctgttccgc gtttggtgac gaggttgatc tgggcgccgg cggcgcgccc ctggtcggaa
     3661 ttcgacatgg ccgtggtgac gcggaattca tcaacggaat cgagtgtttc cctgaggacg
     3721 ccggtaaagg cgtaacccat cttctggtcg ttattgtcta cgccatctat ggtgacattg
     3781 ccctggtcag agcggattcc attcaccgcg cccgtgcggc tgtcgttcat atcgctcggg
     3841 ccgcctacct gattgccgag atacaggacc cccggctgca ggctgagaag gtcaggcaca
     3901 ttgcggtcaa tgatgggcag ttgctggatc gtctgattac tgtaagcatt gcccacgctt
     3961 gcatcggatg tgttgagggt ctcagtttcg gcagacacat tgacggtctg cgagacagac
     4021 tcgaggccca tctggaagtc aatggaggcg ggcttgctga caagcaggtt tccaactttg
     4081 ctttggctgc cgaagccaga tgcgctgacc tttaccgtcc atgtgccggg ggccagttgc
     4141 tgaaactgat attcgccgtg cgagttggtg gtggtgctga gggtctggcc tgtggactcg
     4201 cgcgtgatgg tcacggaagc gttgggaaca agcgcgccgc tggaatccgt gacaactccg
     4261 ctgagcgatg ttgttgcctg ttgtgcgttg actgtatgaa aaacgaagaa aaacactgcg
     4321 aatgccagaa tggcagccgc tgcaatccgc ctgttcataa cgcctccgga gaaaaagccc
     4381 aggttcgcag gaccagttac cgcggtttcc tctggtcctt ttttctttgc acgagagtat
     4441 gcgcccgaat ggctggagaa tcaacagcaa aatgaatcgt ttaaaaaagt ttatatagcg
     4501 gaaaagctac tgcccctggc cttcctgttt agcaacctga ttaatgattc tttgtacaaa
     4561 aaccacgcgc aggtttacgt ggcgcgcaag atcaggaacg aactcggtga tgaagttgcc
     4621 gatggggtcg gtggccaggg cgaccccata acgggacgat gcggccgccc atccggtttc
     4681 gcgatagggc acatacgcat cgctgatgcc ttcttctatg gcgcctccta ttacggcggc
     4741 gtagttgaac atacctttgc cggtgtcgct ctgggtccag accacctgat aaatgcagtg
     4801 cgcgatgcga cgtttataag aagcattggg catgcggtga taatgcgggt cttggtgcgc
     4861 aatggatgga accaggaagg taccgaagaa ctcgttggtc atgtcctgtg tgaaggagac
     4921 gccgctgagt ttggcccatc ctttcatgcc aggtccatag gcggagtgtg aatcggcccc
     4981 aatggagatt gccgacagcg ctccgatggt caaaaggttg aagggatcta tgacgtcttt
     5041 gcctgcaagg acggccttct gccgcggtgt catggggtgc gcttcggcgg agttcagaaa
     5101 gcgctgaaat ggatcgaatg cgggaccgca gctcattcct ggaggtgatg tttgctttga
     5161 ctgcgtctgc gcgcatgccg gctgttgaga tggaaagggc gaacgcggcg aaaaggccga
     5221 ttttggcggc tgaggggcat tgggaggaaa gctctgtccg gcatctggcg aagatgtaat
     5281 gctggcagac ggcgcatcgg gcaagtccgt ctgtgcgtag agtgggtccc gcgcgcccac
     5341 aaagaagggc acaaggaaaa aggccatttt caaagaaagt aaagggaacg ggccggcatc
     5401 cttccggcaa aaccactgca tactacgttt gacgcataag agaacgtttt tcattcacgg
     5461 ctgtggatgg tggtgatgga agattttttg acgcacatcg ggccaaaact ctgaccatag
     5521 attgccggct gcttctccca gcagcgatat accccatgtc tcaaagacga cggatgccgt
     5581 cgcgctttca tccggatagt aggcgagcgt aagcgccgaa gctattccat ggccaatgac
     5641 gacggaattg ttgaatccac tgctgccgtt gtcacgttgg gtgacaaaaa cgcgtgaggc
     5701 ggcgtagagt ccgcgatgtg cgatgctgcc atcgcgctta cggaaatagc gtgggtcttc
     5761 gcgcagcagg gcggggagaa taccgtcaga aaatgccctc atgcccaggt cgcggatggc
     5821 cgctgctccg aatcgctgcc cgaatgctcc ggaatccgtc ccatatttgg ggtgatcatc
     5881 cacaagctgc ccccaaccgg ctgaaagaaa tggagcaaac catccggtaa ggcgcagttc
     5941 ttcgtggata gggaacatca gcttgtcttt tgaataaagc ggcgggacct tttctcccgg
     6001 ctcaacaatg tcagaccatt ttcgttttgc gacagcctgc ggagtattgt tctgcggggc
     6061 atcgggcaac gtttgctgcg agtaacaaca tgcggcagaa aagagaaaca gaaaaagtcc
     6121 tgcagaaaag agctttgata gtttgtgcat tgagtcaagg ggcggacaag tatttggaag
     6181 tcagaatgcg aggcgaggat gtatgccgtt tcaacaaagg taacgccttt ggcacctgtg
     6241 caggcgtaac ttattgacct tgcgaaagca ggaaagcaca ctgaaaaagg ccagaagcct
     6301 ttgggattgg cgcgagaagg agcagcgcac acatggcgga aaacaagttt gaggaactcg
     6361 ggcgcaaggt ggagcaaaag atgaacaccg ttacgccgcg aatcgaggaa gagataaaga
     6421 agatgattgc gtatctcaac gatgaagttg tgccgaaggt gaggcagaac tcatcggaga
     6481 ccatgcgcgc ggccgcacaa caactgcgca aactggccga ctatctggac cggagacagc
     6541 cttgatggcg tacagagcaa aattctcttt tgttgcgatt gcagcaactg tagcgattgc
     6601 cggatgctcg cacaaacaga gcgcggtcta tgtccctcct ccgccaccct cagtggctcc
     6661 agccccgtca tcgccggcaa ccacagcgaa tgttccgccg ccatctctcg ggcctgggcc
     6721 ggcggcggca gacagcacgg cagatgcgga ttttgtggcg acgcataggc ccatttattc
     6781 agaaacagga atggcaagct ggtatgggcc gccgtatcac aatcaccgcg gcgcgaatgg
     6841 ccagatttat gaccagaatg cgctttcggc agcgcatcgc accttgccga tgggttcgct
     6901 gatacgtgtc acgaatctgc ggacaggcca gagcgccgtg atgcgtgtta ccgatcgggg
     6961 gccctttgtg caggacagag tcattgatct ttcagttgca tccgccaaag cggtcggcgt
     7021 gtggaggccg gggaccgcgg aggtccgcat tgatgtatat gctgcgccca agcccattga
     7081 tgatggcgga cggtggtgcg tgcagattgg cgcgttcagc aaagagaagg acgcagtgaa
     7141 gctggaagag cacctggagc acaagtatca cacggcgaat gtcatcgaat ttaccgggcc
     7201 aaccggacat tgggtgcgga tccgtccgct gaacgacgat aagaagcggg cgatggagat
     7261 tgccaaagaa atcaggccgg aagaaggcgg ggcatatctg gtgcggctgg attaggggcc
     7321 tgtgcgcgct ccaacccaca tctctttcta ccccaacacg caaaaatcag gtgttgggtg
     7381 gccccggttc gagatatggg gcatccattg tcatccttgc atgcacttct ttgcacgatc
     7441 gaggagcaat ctgcgttcgc gttcattgcg ggtcatgttt gcggcgcgct caaattcgtc
     7501 tcttgcttct gcgagacggc ccagcttgta gagaaaatct ccgcgcacgc tggggagcag
     7561 atgatagttc ttcagcgatg gatgattcac aagcgcttct gtcagcgcaa gtccggcagc
     7621 ggggccagag gccatgccta cggcaacggc gcggttcagc tccactacag gtgaaggcat
     7681 gatttgtgcg agttctgcgt atagcccggc gatgcggttc cagtctgtat cttcagggcg
     7741 aagcgcgcgt gcgtggcatg cagcaatttc agcctgcagg agataaggcc cgcgcgggcc
     7801 tcccagtgcc tgggcgcgtt cgagggccgc gagtccgcga tggaccagga gctggtccca
     7861 gcgtgagcgg tcctggtcga gcagaagcac agggctgccg tcaggtgcgg ttcgcgcggg
     7921 cagacgggat gcctggattt ccatgagcgc aatcagtgcc tggacttccg gctctccggg
     7981 cagaagtccg gcgaccatgc gtcccagccg cagggcctct tcgcacaggg aagggcgcgt
     8041 ccaggcttct cctgctgtgg ctgtgtggcc ttcattaaag atgagataaa tgacgctgag
     8101 cacagaggaa aggcgcgtgg tcaactccgc cccgcgcggc gtttcaaagg gaacatgcgc
     8161 ttcagccagc gtcttctttg cgcgcacaat gcgctgcgcc agtgttgctt caggaatgag
     8221 gaaggaccgg gcaatctccg gcgttgtcag gccgccgatc atgcgcaatg tgagggcgac
     8281 acgcgcttcc gtgctcagca ctggatgaca cgcggtaaag atgagacgca gcatatcgtc
     8341 gccgatttcg ccgtccattg catcgaaacc gggttgtgcg ttcatctgtt tttcctcaag
     8401 ctcgcggccg atctcttcgt gcttgcgttc gagcattgca ttgcggcgaa agagatctat
     8461 ggcgcgccgc ttggcggttg tcatcagcca tgcggcgggg ttttcaggaa tcccggatgc
     8521 gggccactgc tccagcgcgg aaaccagggc ctcctgcgcg aggtcttctg cgagacctat
     8581 atcgcgggtg atacgcgcaa gccccgcaat gagcttcgca gattcgattc gccagacggc
     8641 ctcaacagcg cgatgtgcat cggtggcagc cacagctacc gatgacatca tgtccacagg
     8701 ctgaattcaa gcatctgctt ctgtcagcaa tccagaactt cacgtatctc aaactcggct
     8761 tctatgttgc cgctctggag agtgggacag cgtttgatcc aggcgattgc ctcttctctg
     8821 gaagcgactt cgatcatggt aacgcccgca atcagctcct tggtctcaga aaatggccca
     8881 tctgtgaccg tgtattttcc tccggacaag tggacgcgag cgccctttgc ggttggctgc
     8941 aatctttcaa cggcaatcag gacgcccgct ttccgcatct cgtcgtcata gcgacgcatt
     9001 gcctcgatga gttcgggagt gacaggcgcg caggactcca ttgttgcatc ggacttccgc
     9061 agaatgacaa aacgcatggt caggaatcct ccattgttag cttctcagtg gaatgattgc
     9121 acgtaaacgc ggttcacgta gatagatgcc ggcccacagc agaatgccga aatataccgg
     9181 gaacagtgtc tcggcaaaca atggatttcc tacacgtaac tgtacggcca ccgctccgcc
     9241 aagatagccc gtgagcagaa tggccccgaa gaccgctgtg cgcgggactg catacagcag
     9301 agtgcaaatg agcaggacga ttccggtggc aaccgtcagg tggccgggcc agcccagttc
     9361 ggcctgtgct ttcagtacgg gcgcgggcct gaataatttc atcgttccat ccataagaag
     9421 aaacaggatg gcgagtgtgc tgaggatgcg tcctgtcatg actcgttttc cggaaacgcc
     9481 tgtgctgttc acaatattgt cctttcaaag ttgagatggg gcttcttctg cctatatgtc
     9541 gaacggcaag accgcaaaac gacatccacg ggtgaaaaaa acatgctaga ctcacacgtt
     9601 cgccatgagc tacgaaaaca ttctcgttga aatgcgcggg gccgctgccc tggttacgtt
     9661 aaaccgtcct aaagtgctga atgcgctgaa tgtggcaaca gtgcgggaac tcgacgccgc
     9721 gatcgcggaa ctggcgggcc acaaggaagt ccgcgccatt ctgcttacag gcgccgggga
     9781 aaaggccttc gccgcgggag cagacattca agagctggcg ctgctcccgg cggtagaagg
     9841 gcaggagctt gcgttgcgcg gacagcaggt ctttgaccgc attgagaact gcggcaaacc
     9901 tgtgatcgcc tgcgtaaacg gctttgctct tggcggaggg tgcgagctgg cgatggcctg
     9961 caccctgcgc attgcaagcg aaacggcgaa gttcggacag cctgaggtga agcttggcct
    10021 cattcccggg tatggcggat ctcagcggct gcctcgcctg attggaaaag gcgctgcgct
    10081 gcgattgctg cttaccggcg agatcatctc tgccgccgaa gccctgcggc tgggtctggt
    10141 ggaggaagtg gttgctccag accagctgct tgcccgcgct gagcagattg cacaggcgat
    10201 tgcagcggtg gctccggttg ctgtgcagaa gtgcatggaa gctgtgcatg cagggtatga
    10261 tcttcccctg cggcaggcgc tggacctgga ggcatcactg tttgggctgt gctgcgctac
    10321 ggaagacaag gccgagggga caaaggcatt tcttgagaaa cgggctgcgg tctggaaggg
    10381 gaagtgacac cccaaaagca gggctcttca cccgtcggct acgtcccatc cttcgtgcaa
    10441 acggcgcgcg aaggacaggg gcacatgcaa aagagccaca agcccttacg atgagaagaa
    10501 agcccgccta attgttcagc atttcctgaa gtttgttgat catccggttc aggtctttgt
    10561 cggcgcgacg ctgttcttca atttttgcga tggagtgcat cacggtggtg tggtgcttgc
    10621 ctccgaactg gcgcccgatc tcgggcagag atgcttcggt catgctcttg gccaggaaca
    10681 tcgcgatctg acgcggcacc accacagagc gggagttgtt cttctgtttg agttcagcga
    10741 ccctcatgcc gaactgctcg gccactgcgc gctgaatggc ctcgatcgtg atcttgcgca
    10801 cctgcgtgtc aatgaactgc ttgaggcatt gctgcgcagc gggcagggtg atttccacgc
    10861 cgctcatgct gcaccatgcg aaaaggcgcg tcagcgcgcc ttccagctcg cgcacgttgg
    10921 tgcggacgtt cgacgcaatg aagaatgcca catccgtggg cagcgtgacg tggtcgttct
    10981 ctgccttttt ctgaaggatg gcgactttgg tttcgaggtc gggcggctga atgtccgcaa
    11041 tcaggcccca ctcaaagcgc gagcgcagac ggtcttcgat ctcggccagt tccttcggcg
    11101 gacggtcgga ggcgatgacg atctgcttca tgttctcgtg cagagtgttg aaggtgtgga
    11161 agaactcctc ctgcgtgcgc tctttttgcg cgaggaactg gatatcgtct atgaggagca
    11221 ggtccacgga gcggtactta tcgcgaaagc tggtcatccg gtcgtaacgc agcgagttga
    11281 tcgtgtcatt ggtgaacttt tcactggtga cgtagcagat ggccgcctgc ggatcgcggc
    11341 gttttacttc atggccgatg gcgtgcatca gatgcgtctt gcccatgccc acgccgccgt
    11401 acagaaagag cgggttgtat gccttcgatg gccgctcggc gacggcctcc gccgcggcgc
    11461 gcgcaaactg gttgccagag ccgatgacga atgcgtcgaa ggtatagcgc gggttgagtt
    11521 gcgccgctgt ggaccagtcg aattttttct gcagaggacc cggcgcagag acagagcgct
    11581 gcgcgctctg cggggccgcg ggcgaatggg caggaacagg agcaaaccca ccgtcttcgc
    11641 gcacgcggac cagcgtgggg tcttcctcag gggtgacgaa gatgacttcg tcaaactcca
    11701 tttgcagatt atccacggct tcctgaatca ggtcgccata acggtcgccg acgtgctgga
    11761 attccggcgt gggaattctt acgtaaagct ttttgccatt gatgtggctg aagcgagtag
    11821 gcttcagcca ggtatcgaac gactgacggt tgatcttctt ttcaagcgcc ccgaggatgc
    11881 gcacccaggg gttcagtgtt gcaggagccg tagcaacaaa agacatcgcg tttcctttat
    11941 ggcacagtcc agctcttcag ttgcgatcag gccgccgctc tggcgcttag cgaaaacaaa
    12001 gtcccggccc ggatgcctat ggcctccggg gagggtccgc tctggtattg ccagcagctt
    12061 atgtgagagt gactttcgct ctcacggcgc gatctgtaga aagcagcaag acgtgcagaa
    12121 aaatctcttg tgaacgttgc cgatagtagc acaattcaga agcgttgaaa ctgttgtttc
    12181 gatgcaaatt ttgtgtcacc ggcaaagttg caccaagtgt tgtgaaaatc aggaattttc
    12241 gtccttctta ttttccgtca gcggcataga atagggcaat attcccgccg tccgtatcga
    12301 aaaaattaat cctgcgctac cagatggaac atcggctcat gggcgccatc ggcgagccgg
    12361 ttttacagcc gaatgctttc tggaattccg gcaggttgac gacgacccca ttgacgcgga
    12421 attcttcggg cgagtgagga tcggtctgca cgcgaaggcg cagattctcc gggcggttgt
    12481 tctggcacca tccctgtcca taggagacaa agaactgctg ctccggagtg agattggcat
    12541 actccggccc ggcctttttc tcgaggttgg tgccgtcctt ttgcgcgcgg tcaagataag
    12601 ccaggaaggc gagcttcaat ccgccgaggt cggctatatt ttcgcctaac gtgagtctgc
    12661 cattcacgtg caggtcgtct acagcgacaa agccatcgta ttcctttacg atgcagtcgg
    12721 cgcgctgatt gaatttcttt tcgtcttcgg gcgtccacca gtctttgaga ttgccctgcg
    12781 cgtcgaactg gcgtccctgg tcgtcgaagc catgggtcag ctcatgaccg atgacgcctc
    12841 cggcgtcgcc gtaattgacg gcatcattct ggctggagtc gaaatagggc ggctgcagga
    12901 ttccggcggg gaaattgatg tcgttcatca ggggattgta atacgcattc acagtaggcg
    12961 gagtcatgcc ccactcgccg cggtcaacag gcttgccaat cttgttgatc tgacggcgga
    13021 cttcaaaagc ggcagcacgc tgtgcgttgc cgagagcgtc tccgcggaca attgtgaggc
    13081 tggagtagtc gcgccatttg tccggatagc cgatcttgtt ggcgaccgcg tgcagcttgg
    13141 cttttgcctg gagtttggtt tcagcgctca tccagtcgag ctggtcaatg tcgcggccca
    13201 tggccgactc gatgtcgcgc gtcagctcca gcgtgcgctg cttgtcctgc ggactgaagc
    13261 gctccgatac atagacttgt ccaagcgcct cgccgagcgc atggtcggtg gcgcggacac
    13321 agcgtttcca gcgcggctgc tgctgttgct ggccttcgag aatttttcca tagaagttga
    13381 acgactcttc atcgagcgcc tccggcaggg ccgttccggg aacctggcga atggccgccc
    13441 agcgcaggta agttttgatc gagtcgagac tggcagaggc gagcacttca ttcaatccgg
    13501 tgaagaagcc gggttcggtc acattgagcg attgcacggg cgggacatcg agcgcccgca
    13561 ggtaacgggc gaaggcgaag tctggcgtgc tgccctcgaa tttttccagg gtcattttgt
    13621 gatagagcgc ctgtgggtcg cgtcgttcta cgttcgtaag agagtgtttg gccaactcgg
    13681 tttcaagttt caggacagct tcggcatctt tggcggcctg cgcctgtggt tcgccgatta
    13741 gctcaaacgt gcgcgccaca tgggccttat attgttcgcg caattttacg gatttgtcat
    13801 ccgtgcgttc gtaatagcct ttttccggaa gcccgagttc ggcctggtca aattcggcga
    13861 tttcctgggt tgcatctttg aagtcctggt cactgccgaa tccaaagaag ctgctgatgc
    13921 cgatgctgtc caggtatgcg gcaagttcgg gcaggtcgtt tttggatttc agggcggcga
    13981 tgcggtcgag gatgggctga agtggtttca atccggcctg gttgatgacg ctggtgtccg
    14041 tgcaggtggc atagaaatcg ccgattttct gctcattcgg cgtgcgcgcg gtaccgcccg
    14101 cggcggccct ttcgagaata gacttcagca cgaggcggtt ctggttggcc agctctgtgt
    14161 cccgcccgta gctggattcg tcggctggaa tcgggttctg cctgagccat cctccgcagg
    14221 aatactggta gaagttcacg cacgggtcaa cgctggtgtc catcagggtg gggtcgaggg
    14281 catggagctt cttttccgaa gcgttctggg cgaacattgc gaaagggcct gcgaaaagaa
    14341 ggcaggaaag cagaaaaagg cgggacagat tcatgatggc aatgatatcc gcaaaatact
    14401 gtttttattt atcttctttt tgcagttgtc cgcgcattcg gatatacttg gaaattgctg
    14461 cgtagagcgt ttaccgcttt gtctgtagcg caatttccat tgagattttg aggagcgcac
    14521 ttccatgccg aagcgcacat ttcaacccaa tcgccgccgc cgctcgaaga cgcacggttt
    14581 ccgcacccgt atgaagacca aaagcggtgc agccgtcctg tcccgccgcc gcgcaaaggg
    14641 ccgtcatcgc gttgccgtca gcgcgggcca tcgcgactag tttctggtct tcttccgctg
    14701 tgattcactc cggtattccg ggaaaagcca caaagccatg caggaacaag cttctgaacg
    14761 gacgccgcgc acgctgcgtg gcgctcgcct cagcaggcat tccgattatc agcgggtcta
    14821 ccagcagagc cggaagtatt tctctgcttc gatgacatat ttcttttacc ggcgcgagga
    14881 cggcctccac tccgggccgc gcattggcct gactgccgga cgcgtgctgg gaaaagccgt
    14941 cgagcggaac cgcatcaagc ggcgcatgcg cgaggccgcg cggctgaacc tcgagcatct
    15001 gcgggcagat gtggatgtgg tgcttcatcc ccgaagaagc gttctggaga tggatttctc
    15061 acgcttgcgg gacgaagtgg gcgaaatctt tgcaaagatt gaggcggctg tgaagcaaaa
    15121 ggagaagagc gcatgaactc ttcccggcgt tccatcgtat ctcctgtagc ggccgtgcat
    15181 gtgatttata aatttgtgct ttctccgctg ctgcacctgg gggcgggcat gtccggtggc
    15241 gcgtgccgtt ttcagccgac ctgctcggag tatgcgaaca tcgccattca tgaatacggc
    15301 ctgctgcgcg gcggatggat ggcgctaaag cgtttttcgc gctgtcgtcc cggctgcgcg
    15361 gggggctatg accccgttcc ttacacagac cgccaaagcc agttaccata aaagaagcgc
    15421 tgaagatccc ataggaagta tctcttggca gaaatccaaa atccgaacca gcagggcggt
    15481 gggcaggact cgaaaacgct gctcacgttc actcttgttt tcttcgtact gtttcttggc
    15541 ctgcagtttt ttatgaagcc gaaaaagcct gaaactcctg cgcagaacca gccacagcag
    15601 cagcaacagc aaaagcagga agcggcagac aatcccgcgg cctcagcggc tgccccggtg
    15661 aatgtgtctt caggtccggt gatccaggct gaggcggaat ccacgacggt ggttgagaac
    15721 gatctatacc gtatcacctt caccaatcgc ggggcacagg tcacttcatg ggtgctgaag
    15781 aaatataagg atgacgatgg caagccgctc gatctggtga accatgcggc ggccgcgaaa
    15841 tacggatatc cgctttcgct ttatacctac gatgcgaagc tgcgcagcca gcttcagcag
    15901 gcgttgtttg tgccgtcagc gacaggcagc gtcaaagcgc cggggacatt gagctttgag
    15961 tattcgtccg gcggcctgtc ggtacacaag actttccggt ttgatggaag ttatgtcatt
    16021 catgccgatg tgtctgttac gcagaatggc actcctgtgg ctgctatgct tgcttggccc
    16081 tccgggtttg gcgaccagaa cacgctgccg gattatgcca aggccactgt ggtcaccgca
    16141 cagagcggca aggccgacca gattccggca aagaaagtat ctggaggcga gacactgcag
    16201 gggccgtttg actgggccgg agtcagcgac ctgtatttcg gagcattctt cctgccggaa
    16261 aatccgaaag acctgagtct ggtaagtctg cacgatacca ttgccattcc gcagaacagg
    16321 aagaaccctg atccgggccg cacggaaaaa gcttcagtac ttggcgctgc catgggcgct
    16381 gcgcaaccgt acagtgtgcg gctctttgcc ggccccaagg aactggatgt gctggcttcc
    16441 gttcctacca gtggagtggg actgcaaaag gcggtgagtt tcggcatgtg ggggccgatc
    16501 tccaagccgc tcttccttgt cctgcgcttc ctgtatgagc atgttgtccc gaactggggc
    16561 tgggcgattc tgattctgac ggtcatcctc aaccttgcca tgctgccgac gcggttccag
    16621 atgatgaaat catcgctcaa gatgcagcgc attcagccgc agatagaggc catcaaggca
    16681 aaatatgcca agtacaaggc caccgatccg cgccggcagg agatgaacaa agaagtattt
    16741 gaattgcagc ggcgcgaagg cgtgaatatg ttcggcggct gcattccgat gctgatccag
    16801 tggcccctgc tctatgcttt ctaccgcatg ctggaaaacg tgattgagct gcggcaggcg
    16861 cactggctgt ggctgcctga tctttccgct cctgatccgc tgcatattct gccgatattc
    16921 tttatcgttt cgatgttcct ggtgcagtgg cttacgcctt ctccgggcgt agacccttcg
    16981 cagcaacgga tgatggcgtt gaccatgccg gcggtcttcg ggttcatgac gtggaatgtt
    17041 ggctcaggtc ttgcactcta ctgggcgggc agcaatattc tgggcattgt ccaacagatg
    17101 ctgatgaacc gcaccaaact gggtagggag atgcgcgcac tggcactgaa gcgagcggcc
    17161 aagcgcgccg ggaagcctgc actcgcgcgg cgatgagggc cctcagaaaa tccattgaag
    17221 tctcgtgtgc ggcattgaga cgggattagc gccaaatcca gccctgatga gatacttaag
    17281 ccgatgccaa ttgacgatta ttctgccgcg gcccagaaga ttgccgcgtt tctgaaaaac
    17341 ctgactgccc tgggcggact ccgtttgaag tacagaatta cggcaggggc aggcgccgcc
    17401 gatcctgaag ggctggaagc gcgagaaatt tatgtggagc ttgctggtcc cgatgcttat
    17461 ctgctgacgc agcgcggagg tgaactgctc cgcgcgttgg agcatgtcgc cgccaaaatc
    17521 gtccgcctgg aggcagatga acacgacaag atttcatttg acgcgaacaa ttttaagacc
    17581 ctgcgcgcgc gcgagctgaa gatggccgca gagactgcgg ctgataaggt ccggcgtacc
    17641 ggacagccct acagctttgc tcccatgagc tcgcgagagc gccggatgtt gcatctcgcc
    17701 ttccgcagct atgacgatgt ggagactgca tccagcggcg aaggactgcg ccgctatgtt
    17761 gttgcctatc cgaagggaca cgcgcaggcg aaggcgcaaa tgcgcagata gct
//
LOCUS       DASRBV010000070         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_510219, whole genome shotgun sequence.
ACCESSION   DASRBV010000070 DASRBV010000000
VERSION     DASRBV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510219"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="murG"
                     /locus_tag="VFA02_02915"
     CDS             complement(<1..947)
                     /gene="murG"
                     /locus_tag="VFA02_02915"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZU08829.1"
                     /translation="MSEGPCDSSYQSGNMMRVLIAGGGTGGHIMPALAIAEALRQKYQ
                     TELLFVGTPRGLESRLVPQAGYHLELIQVGQFNNVSLATRLRTLLDLPASILHCRKLL
                     KQFHPDVVISVGGYASGPATIAAILAHVPTLAVEPNAYPGMANRLVGKHVSAAAVNFR
                     PALKYFRNAQVTGIPVRSEFFRLQPRPADGPPHLLVFGGSQGARALNRVVPLIAARLL
                     EAVPGLTILHQAGARHAEETQAAYAAGGADPARWQVHAFLDDMPKRFEAADLVLSRSG
                     ASTVAEEMAAGKPALLVPFPGAADDHQRKNAEVMAAANAA"
     gene            complement(1001..1417)
                     /gene="ndk"
                     /locus_tag="VFA02_02920"
     CDS             complement(1001..1417)
                     /gene="ndk"
                     /locus_tag="VFA02_02920"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZU08830.1"
                     /translation="MSQRTFSIIKPDAVRKGHSGAILAEIEKAGFRLIAIKKLSISKT
                     QAEGFYYVHKERPFFGSLTDFMSSGPIFVMVLEKENAIADLRKLMGATNPANAEEGTI
                     RKKFAASIEENAIHGSDGEDTAKFEIGYFFAGYELV"
     gene            complement(1561..2436)
                     /locus_tag="VFA02_02925"
     CDS             complement(1561..2436)
                     /locus_tag="VFA02_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU08831.1"
                     /translation="MQEISRRSLLKTGSAALAAFGVGGAKLFANPLGLPIGLQLYSVR
                     ALLPKDFAGTLKQLSAIGYKEVEAAGFFDHSAEDVKKAMSDAGLRCVSAHYPMPVLQK
                     DPEGTLRYAKNLGLKYIVCSSPSVADPSAGRHELTADDWRWNAEQLNRFGKQFKAEGI
                     RMGYHNHTPEFRDLGEGKIGYDVLLKDTDPALVTFEMDCAWVVAGGRNPADYLHQYPN
                     RISMLHVKDLKPVSGNSTERTSTVLGKGTIDYKPIFAAAKKANIQHYFVEQEEFDGDP
                     LEELRQDYQYLHALR"
     gene            2494..>2858
                     /locus_tag="VFA02_02930"
     CDS             2494..>2858
                     /locus_tag="VFA02_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU08832.1"
                     /translation="MEQFSLNNTQVSRIGLGTWAIGGSEWGEVPEKDAIATCLAALDY
                     GINLIDTAPIYGHGRAEEIIGKAMRQHGQRDKFYIATKAGLEWNERGVYANTTPARLR
                     KELEDSLRRLGTDYIDLYQ"
BASE COUNT          616 a          805 c          811 g          626 t
ORIGIN      
        1 gccgcattgg ctgccgccat gacctcggcg ttcttgcgct gatggtcgtc agctgctccg
       61 gggaagggga ccagcagcgc gggcttgcct gcggccattt cttctgcgac tgtactggct
      121 ccgctgcggg agaggacaag atcggcggcc tcaaagcgct ttggcatgtc atcgagaaag
      181 gcatgcacct gccatcgtgc tggatcggcc ccgccggcag cgtaggctgc ctgtgtttct
      241 tcggcatgac gcgctcccgc ctggtgcaga atcgtcaggc ccgggacggc ctcgagcaga
      301 cgcgcggcaa tcagaggtac gacgcggttc agcgcgcggg caccctggct tccgccaaag
      361 accagcagat gaggcgggcc atccgcagga cgtggctgta gacggaaaaa ctccgagcgc
      421 accggaatac ctgtgacttg cgcgtttcgg aaatacttca gcgcgggtct gaaatttacg
      481 gccgcggcag aaacatgctt gcctaccagc cggtttgcca ttcccggata agcattcggc
      541 tcgactgcca gcgtaggaac atgggcgagg atggcagcta tggttgctgg accggaggca
      601 tatccaccca cgctgatgac cacatcgggg tgaaactgct ttaagagctt gcggcaatgc
      661 agaatgctcg cgggaaggtc gaggagtgtc cgcaggcgcg ttgccaggct gacattgtta
      721 aattgaccca cctggataag ttccagatgg tatccagcct gaggtacgag gcgggattca
      781 agcccgcgcg gagtgccaac aaagagaagc tcagtctggt atttctgcct gagggcctcg
      841 gcaatggcga gggctggcat gatatggccg ccggttccgc ctcccgcaat gagaacgcgc
      901 atcatatttc cactttgata gctggagtcg catggacctt cgctcatgcg actccttcca
      961 ctgctcagca gcctccactc aaggatgaca aattgtctgc ttagacgagt tcgtatccag
     1021 cgaagaagta gccgatctcg aactttgcgg tgtcttctcc atcggagccg tggatggcgt
     1081 tctcctcaat tgaagcggcg aacttcttcc ggatggtgcc ttcctcggca ttcgccggat
     1141 tggttgctcc catcagtttg cgcaggtcgg cgatggcgtt ttctttttcg agcaccatca
     1201 cgaaaatcgg ccctgaagac atgaagtcgg tgagtgaacc gaaaaacggg cgctctttat
     1261 ggacataata gaagccttcc gcctgcgtct ttgagatgga aagcttcttg atggcaatga
     1321 gcctgaaccc tgccttttca atctcggcga gaatggcccc ggaatggccc ttgcggaccg
     1381 cgtctggctt aataatgctg aaggtgcgtt gggacacctg gtcgaatctc cttgcaaaaa
     1441 gtgcgttagt tccagtgtaa agcactgtcc ggtcctgtca ttcttacggc cgcggggacg
     1501 aagaatcctc gcaatataaa ctcgccaatc gcgttcacac agaatgacag gcccgccaac
     1561 tcaacgcagc gcgtgcaggt actggtaatc ctgccgcagc tcttcaagcg gatcgccgtc
     1621 gaattcttcc tgctctacaa agtagtgctg aatgttcgct tttttcgcgg cagcaaagat
     1681 gggtttgtaa tcaattgtgc cttttcccag tacggtcgaa gtccgctcgg tagagtttcc
     1741 tgaaacaggc ttcaaatctt taacgtgcag catggaaatg cggtttggat actgatgcag
     1801 atagtccgcc ggatttcttc ctccggcaac tacccaggcg cagtccattt caaacgtcac
     1861 cagcgcgggg tcggtgtctt tcagcagcac gtcgtaacca atctttcctt caccgaggtc
     1921 gcgaaactcc ggcgtatggt tgtgataacc catgcggatg ccctctgcct taaattgctt
     1981 cccaaagcgg ttgagctgtt ctgcattcca gcgccagtcg tccgccgtga gttcatgccg
     2041 cccggcagat ggatcggcaa ccgagggtga agagcagaca atatatttga ggcccagatt
     2101 tttcgcatac cgcagggtcc cttccgggtc tttttgcagc acgggcatgg gataatgcgc
     2161 gctcacgcag cgcagtcctg catcagacat ggccttcttt acgtcctctg cgctgtgatc
     2221 aaagaaccca gcggcctcca cttccttgta tccgatggca gagagctgct tcagcgttcc
     2281 tgcaaaatct ttaggaagca gagcgcgcac gctgtagagt tgcagcccaa tgggcaatcc
     2341 aagcggattg gcaaacaact ttgctccacc cactccaaac gcagccagcg ctgcggaacc
     2401 agttttgaga agcgaccgcc gtgaaatctc ctgcacatgt cctccttcga ttaaggcgtt
     2461 ttagcactcg acggcattgt aatctgtttg cttatggaac agttctctct gaacaacaca
     2521 caggtttcaa gaatcgggct gggcacgtgg gccataggcg gaagcgaatg gggcgaggtc
     2581 ccggaaaaag atgcgattgc cacgtgtctt gccgctttag actacggcat caacctgata
     2641 gatacggccc cgatttatgg ccacggacgc gcggaagaaa tcatcggcaa ggccatgcgc
     2701 cagcacggcc agcgcgacaa attctacata gccaccaagg ccggcctgga gtggaatgag
     2761 cgcggagtct atgcgaatac aactccggcg cgcctgcgaa aggaactgga agactcactg
     2821 cggcggctgg ggaccgacta cattgacctc taccagat
//
LOCUS       DASRBV010000071         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_612025, whole genome shotgun sequence.
ACCESSION   DASRBV010000071 DASRBV010000000
VERSION     DASRBV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612025"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="pstS"
                     /locus_tag="VFA02_02935"
     CDS             <1..711
                     /gene="pstS"
                     /locus_tag="VFA02_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZU08833.1"
                     /translation="GLEHPLQLSPQALAAIFLGTVKSWQDPAITKDNPGVQMPKLPVI
                     VAHRADGSGTTNIFTTYLSAVSSEWQSKVGKGNSVSWPVGIGGKGSEGVTGNIRQSPG
                     AIGYVELTYAQQNHLPVAKIKNQAGEYIAPTAQATTSAINAFSSQLAQDPRTPIVNPP
                     ATAKDAYPISGLTFLIIPKDGQGKVKRSALKGFVQYVITDGQATAGSLSYAPLPDGVK
                     QYDQQQLQQLTANGLPVQ"
     gene            complement(772..2805)
                     /gene="argS"
                     /locus_tag="VFA02_02940"
     CDS             complement(772..2805)
                     /gene="argS"
                     /locus_tag="VFA02_02940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZU08834.1"
                     /translation="MYLTEQKHLLDRLRDVLRVKYQIELENLAIEQPPDIKFGEYALP
                     VAFELARLLRKPPRKIAEEIVAELAPLPGFAGFEVAGAGYINAKLDRGRAAPEIAAGK
                     EILSPETPLHALVEHTSINPNKAAHVGHLRNAILGDTFVRLLRAAGHKVDVQNYIDNT
                     GVQVADVVVGLMHLEKLSLDGVRALMQQLEQRGEKIDYYCWDLYARVSQWYDADESQK
                     AARKQIRLDTLHAIEEGTSEAAQIGDLISTAILRRHLETMLRLGIEYDLLPRESEILH
                     LHFWNLAFEQLKESGVLYYEEEGKNKGCWVMRRPGTGTGRPGGVLRETQDADAAEENG
                     SQGPDEDAKVIVRSNGTVTYVGKDIAYHLWKFGLLGRDFAYRKFFEYPNHTCWISAET
                     GEPDHPHFGGAQAIYNVIDSRQADPQANVIEALRGLGYTEQASRYTHFSYEMVALTPR
                     CAEELGYEISEEDRGRTYIEVSGRKGFGVKADDLIDKLIAATQKEVDGRHPELGAAER
                     QQIAQKIAVGALRYFMLKYTRNTVIAFDFNEALSFEGDTGPYLQYAVVRTRSIFRKAG
                     TTAEHALADFTGVDATPYLSGEDGDGIWQVWLRAAKTSLLLDQCIATAEPAYLAKHAF
                     QLAQEFSNFYRKHHILTEPDPERKKFLLATAAITQRELIRSLEWLGIGAPEVM"
BASE COUNT          595 a          866 c          787 g          641 t
ORIGIN      
        1 ggtctggaac accccctcca gctttctccc caggcgctgg cggccatttt cctgggtacg
       61 gttaagagct ggcaggaccc ggccatcacc aaagacaatc ccggcgtgca gatgccgaag
      121 ctccctgtga ttgtggcgca ccgcgccgac ggcagcggca caaccaatat cttcaccaca
      181 tatctctcag ccgtcagttc agaatggcag tcgaaggtcg gtaaaggaaa ttccgtaagc
      241 tggccggttg gaatcggcgg aaaaggcagc gaaggtgtga ccggaaatat tcgtcagtcc
      301 ccaggggcca ttggttatgt tgaattgact tacgcccaac aaaatcatct gcccgtagca
      361 aagataaaaa accaggccgg agaatacatc gcacccacgg cacaggccac aacttcggcc
      421 atcaatgcct tcagcagcca gttggcgcag gatccacgca ctccgatcgt gaatccgccg
      481 gccacggcaa aggatgccta tcccatttcg ggcctgacat tcctcatcat ccccaaagac
      541 ggccagggca aggtgaagcg ttccgcgcta aagggctttg tgcagtatgt catcacggat
      601 ggccaggcga ccgccggttc actgagctat gctcccctgc ccgacggcgt aaaacaatat
      661 gaccaacagc agctacagca attgacggcg aatggcctgc cggtgcagta gtcgttagtt
      721 gttagtcccc agcccccggt acttactggc aaatgggggc tgggactcga tttacataac
      781 ttcaggcgcg ccaattccca accattccag tgagcgaatc agctcccgct gcgtaatcgc
      841 ggccgtcgcc agcagaaatt tcttccgctc cgggtcaggc tcggttagga tgtggtgctt
      901 gcgataaaaa ttgctgaact cctgcgcaag ctggaaggcg tgttttgcca gatacgcggg
      961 ttctgccgta gcaatgcatt ggtcgagtag cagcgaggtc tttgcagcgc gcaaccacac
     1021 ctgccagatg ccgtcgccgt cttctccgct aagatacggc gtggcgtcta ctccggtaaa
     1081 atcggcgagc gcgtgctctg ccgtggtccc ggctttgcgg aagatgctgc gcgtgcgcac
     1141 aacggcatat tgcagataag gccctgtatc gccttcaaaa cttagcgctt cattaaagtc
     1201 aaaggcgatg acggtattgc gcgtgtattt cagcatgaag tagcgcaggg cgcccacggc
     1261 gattttctgt gcaatctgct ggcgctctgc tgcacccagt tcaggatggc ggccgtctac
     1321 ttccttctgc gtggccgcga tgagcttgtc tatcagatcg tcggccttta cgccaaagcc
     1381 cttgcgccct gagacttcga tgtacgtccg cccgcggtct tcctctgaga tttcgtagcc
     1441 cagttcttcc gcgcatcgcg gagtcagcgc caccatctca taggaaaaat gcgtgtaccg
     1501 gctggcctgc tcggtgtatc ccaatccgcg cagcgcctca atcacgttcg cctgcgggtc
     1561 agcctgccgc gagtcaatca cgttgtagat ggcctgcgct ccgccaaagt gtgggtgatc
     1621 cggctcaccc gtctcagcag aaatccagca ggtgtggttc ggatattcga agaatttgcg
     1681 ataagcgaaa tcccgtccca gcaggccgaa tttccagagg tggtaggcga tgtccttgcc
     1741 gacgtaggtt actgttccgt tcgagcggac gatgaccttg gcgtcttcat cggggccctg
     1801 gctgccattc tcttccgccg cgtctgcatc ctgagtctcg cgaagcacgc cgcctggacg
     1861 gccagtcccg gtgccagggc ggcgcatgac ccagcagcct ttatttttgc cctcttcttc
     1921 gtaatagagg acaccgcttt ccttcagttg ctcaaaggcc aggttccaga aatgcagatg
     1981 aaggatctcg ctctcgcgcg gcagcaggtc gtattcaatg cccagccgca gcatcgtttc
     2041 cagatgcctg cgtagaatgg ccgtcgaaat gaggtcgcca atctgcgcgg cctcgcttgt
     2101 gccctcttca atggcgtgca gcgtatcgag ccgaatttgt ttgcgtgccg ccttctgaga
     2161 ctcgtcagcg tcataccact gcgagacgcg cgcatacagg tcccagcagt agtaatcaat
     2221 cttttcgccg cgctgctcta gctgctgcat cagcgccctg actccatcga gcgacaactt
     2281 ttccagatgc atcagcccca cgacgacatc ggccacctgc accccagtgt tgtcaatgta
     2341 gttctggacg tccactttgt gtcctgctgc cctcagcagc ctgacaaatg tgtcaccgag
     2401 gatggcattg cgcagatgcc ctacatgcgc ggccttattg ggattgatgc tggtgtgctc
     2461 caccagcgca tgaagcggcg tttccggcga gagaatttct tttccggcag caatctccgg
     2521 cgccgcccgg ccgcgatcca gctttgcatt gatgtatccc gccccagcga cttcaaatcc
     2581 cgcgaaccct ggcagggggg ccagctcggc cacgatttct tcagcaattt tgcgcggagg
     2641 ctttcgtaac aggcgtgcca gttcaaaggc cacaggcaga gcatactcgc caaacttaat
     2701 gtccggaggc tgctcgatgg ccaggttttc cagctcaatc tggtatttca cgcgcagcac
     2761 atcgcgcagg cggtccagca gatgtttttg ttctgttaaa tacacgtgga aagaaaagac
     2821 cagtcaaaag aagttgattt tcctgcctga ttttatcagg cagatgcgcg gcagggactt
     2881 ttgcaaaat
//
LOCUS       DASRBV010000072         8031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_510236, whole genome shotgun sequence.
ACCESSION   DASRBV010000072 DASRBV010000000
VERSION     DASRBV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510236"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFA02_02945"
     CDS             complement(<1..1565)
                     /locus_tag="VFA02_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08835.1"
                     /translation="MAMEHKYEDDIQKLIDTGKEKGYLTYGEVNDLIPNDLNSPDDLD
                     DLITTIGTQGIDLLDEGSPKFGGGGYEEGEEGEDVELDLTPGALEKTNDPVRMYLREM
                     GTVPLLTREGEVEIAKRIERGQLRVMKAISRSPIVIREIVALGEDLKRGVRNIKEVVI
                     FDEEEITEEVLQARIKQTVAKIDALVKHQKKAAQLEEKLAAIAGQKTKAKEQRKLRWA
                     IARERIYGHRIIRELKYTNQERKRLLDKVNKTVDSMRMLERQIKSLDQKYDASKSEEL
                     KKEYRRQQKNCKIDLEKLEQEAGVTIAELKRTQREMIQGDMDAEQAKRELIEANLRLV
                     VSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIAD
                     QARTIRIPVHMIETINKLIRTSRQLVQELGREPTSEEIAKRMDIPVAKVRKVLKIAQE
                     PISLETPIGEEEDSHLGDFIEDRMAVSPSDAVISVNLKEYTSQVLRTLTPREERVIKM
                     RFGLEDGSEHTLE"
     gene            complement(1991..2815)
                     /locus_tag="VFA02_02950"
     CDS             complement(1991..2815)
                     /locus_tag="VFA02_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08836.1"
                     /translation="MHRFHKRIFFALLAGCTIFPANAQQSFVNRWQERVNRTQAEQPH
                     WVTPLATVTPRLEQEFRTDFMRQITPTHTDTWNYGGGKGLELIPTRRIELLLNVPPYI
                     QHNSKAKDGFGDASFVMKYRFYASNEEGHNAIVTAFLGGSIPTGSYSNGSTDATVTPT
                     LAGGKGFGRFDVQSTLGATLPVANSRKLGRPIAWNTAMQWKANPHWWPEVEFNSTFFK
                     GGPNDGKIQNFVTPGIVSRWPLHRRVGVTLGAGMQMATSTYHSYNHGLIFSARMPF"
     gene            3044..3679
                     /locus_tag="VFA02_02955"
     CDS             3044..3679
                     /locus_tag="VFA02_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU08837.1"
                     /translation="MSKPRDPEDSKPLFGKFTRRSFLTQLGAAGLAVTTAPLANAAPA
                     APQTEAETAAAEPGAIPVILKVNGQTHKLMLEPRVTLLDALREDLRLPGTKKGCDHGQ
                     CGACTVHVNGRRVNSCLSFAVMHQGDEITTVEGLAQNGDLHPIQAAFIEHDGYQCGYC
                     TSGQIMSAVALLKEPIGREDNDVREAMSGNICRCGAYNNIVAAVQQVRRSA"
     gene            3698..4678
                     /locus_tag="VFA02_02960"
     CDS             3698..4678
                     /locus_tag="VFA02_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZU08838.1"
                     /translation="MQTFQYSQAKSPEAAVSVAGQQTRFIAGGTTLVDLMKLNVERPA
                     SVIDINGLPLDRIEPTSDGGLRIGALVRNSDLAWNAEVKERYPVLSEALLSGASPQLR
                     NMATTGGNLLQRTRCYYFRDTNYKCNKREPGSGCSAIDGFHRIHAILGTSEHCIATHP
                     SDMAVAMMALEATVHTRGAKGERSIPLNEFYLVPGTTPEKENVLEPGELITYVTLPAL
                     PHGTKSHYLKLRDRASYEFALASAAVVIQTSGGKIQFARVALGGVGTKPWRSKEAEQE
                     LHGREANERTFRAAAEAALKPAKPLRDNAFKVELAKRVIVRALQTTTQTA"
     gene            4692..6908
                     /locus_tag="VFA02_02965"
     CDS             4692..6908
                     /locus_tag="VFA02_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZU08839.1"
                     /translation="MSTSIIGSAVGRVDGRLKVTGAARYAVDHPLENIAYGAGVFSTI
                     ANGKIVRIDTSEAEKMPGVIAILYHGNSEPIYRPAGQFEHSRASEARPPFEDDQVYYY
                     GQFVALVVANTFEQAQDAASRVRVTYAEEKPVVHLEDAPTGEQVANYSRGNAESAFES
                     APVQIDETYITPVETHNPMEMHGTIAVWNGDKVTLYETTQGVVNHQQVMSEMLGVPLE
                     NVHVISPFCGSGFGGKLFPWPQSMLAAIAARRVKRPVKLYVPRSMMFTTVGHRPYTKQ
                     RMRLGATRDGKLVSIQHDVLSTTSKVDTYVEHCTSSTPMLYSCPNVKAVQHLVPLHVG
                     TPTPMRGPGTVPGLFPLESAMDELAIKLNMDPLELRLRNYAEKDEGQDLPFSGKHLRE
                     AYQTGAERFGWSRRNPKVASMRNGDLVLGWGMSTATWHAGRGGATVRVRLNADGTARA
                     SSATQDIGTGTYTVIAQVVADKTGIPLDKIEVVLGDSSLTPGPMSGGSTATASVMPAV
                     AGATNNAVNTLIQIAQRTPGSPFYVKETPGGDPPTLKMTNGYIHAADKAPDSGVPFHE
                     VLAMRRLSGIDGEAKTGPAEGMHKFSGHSFGSHFCEVTWDPGIARLRISRWVTVMDAG
                     RIINKKTATNQILGGVVMGIGMALFEETLYDPRNGKPLNNNFADYLVSTNADIPQMEC
                     IFIEYPDLNLNEYGARGVGEIGLAGVASAITMAVYHATGVRVRKLPVRIEDLLGHV"
     gene            6943..8013
                     /locus_tag="VFA02_02970"
     CDS             6943..8013
                     /locus_tag="VFA02_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZU08840.1"
                     /translation="MSELKQIVSLWRTAKARREEVCLATIVRTEGPSYRKAGTHMLLI
                     RGGQRAGTISGGCLEGEVSKKAWWLTEKGPTVQRYSSFFDDDSPLPYGSGCGGTVYIL
                     LDRGPAAHAILDALESSLTKRMGFVAIIDTNQGKTLLVQQSNGAVLFGDQSGVGQLAE
                     QAFRERRSLYAPDAPEIFVEYTAPPAALTIFGAGDDAKPLSEFAERIGWHVVIADGRS
                     HLARKERFPDADRVHVLDYESPAPLSTLDLDPEHAFVLMTHSYEQDRALLRELLPLAP
                     KYLGVLGPRARTERIVCEIADAVHLSPEECLARLHSPVGFDIGAHSPEGIALAIAAEI
                     FASLQERGPANQKYHHLLHVSK"
BASE COUNT         1648 a         2420 c         2261 g         1702 t
ORIGIN      
        1 tcttcaagcg tgtgctcgga gccgtcctca aggccgaagc gcatcttgat cacgcgctct
       61 tcccgtggcg tgagcgtgcg cagcacctgc gaggtgtatt ccttcaggtt cacgctgatg
      121 acggcatcgg aaggcgatac agccatgcgg tcttcgataa agtcgcccag gtgcgaatct
      181 tcctcttcgc cgatgggcgt ttcaagcgag ataggctcct gcgcaatctt gagcaccttg
      241 cggacctttg ccaccggaat gtccatgcgc ttggcaatct cttccgaggt aggctcgcgc
      301 cccagctcct gcaccagttg gcgcgaggtg cggatgagct tgttgatcgt ctcgatcatg
      361 tgcacgggga tgcggatggt gcgggcctgg tcggcaatgg cgcgggtaat ggcctgacgg
      421 atccaccatg ttgcataggt cgagaacttg tagccgcggc ggtactcgaa cttgtctacc
      481 gccttcatca ggccaatgtt gccttcctga atcaggtcaa ggaactggag gccgcggttg
      541 gtgtacttct tcgcaatcga gaccaccagc cgcaggtttg cctcaattag ttcgcgcttg
      601 gcctgttcgg cgtccatgtc gccctggatc atctcgcgct gggtacgctt cagttctgca
      661 atggtgacgc cggcttcctg ctccagcttt tcgaggtcaa tcttgcagtt cttctgctgg
      721 cggcggtact ccttcttcag ctcttcgctt ttcgaggcgt catatttctg gtcgagcgac
      781 ttgatctgcc gctccagcat gcgcatcgag tccactgtct tgttgacttt gtcgagcagc
      841 cgcttgcgtt cctggttcgt atacttcagc tcacggataa tgcggtgccc gtaaatccgt
      901 tcgcgggcaa tggcccaacg gagcttgcgc tgttcctttg ctttggtttt ctgaccggca
      961 atggcagcca gcttttcttc aagctgggcg gccttcttct ggtgcttgac cagagcgtca
     1021 atcttcgcga ctgtctgctt gatgcgcgcc tggagcactt cttcggtgat ctcttcttca
     1081 tcaaagatca ccacttcctt gatgttgcgg acgccccgtt tcaggtcttc gcccagcgct
     1141 acaatctcgc ggattacgat aggtgagcgc gaaatggcct tcatcacgcg caattgcccg
     1201 cgttcgatgc gcttggcaat ttctacttcg ccttcgcgcg tcagcagcgg cacggtaccc
     1261 atctcgcgca gatacatgcg gacggggtcg ttggtcttct cgagggcccc cggagtcagg
     1321 tcaagctcaa catcttcgcc ttcttcacct tcctcgtatc cgccgccgcc aaacttgggg
     1381 ctgccctcgt caagcagatc aattccctgc gtcccgatcg tcgtgatcag gtcgtcgaga
     1441 tcgtccggtg aattcaggtc attcggaatc aggtcattga cttcgccgta agtcagatag
     1501 cccttttcct tccctgtatc aatcagtttc tggatgtcat cctcgtactt gtgttccatc
     1561 gccaagcggg tcgtctcccg tggaaaattt cggatcgtgt ttatgcttgg atcggacgag
     1621 gtgcggttga ggccatatca ggcatgtccc ggagcgatcg ggtcgcaggg ttaaaggcgc
     1681 acgtccacca aagactttta gaataccacg gacgggacaa ttatcataca ttccgcgcgt
     1741 ccggagttcc ttgatcccaa tgaattagat gtggaaaacg ggattttgtt tctgtaatct
     1801 gcttaggaat tactgattgt ccgaaagggc ggcggcgtct tcttccaggc cagccataac
     1861 cataacagaa tcagcacaaa gcgctgttat ccgccttggg cttgctgaaa cgtgcagcag
     1921 aaaaaggacc gcatatctgc ccgaaacaga actactttta tcccttgcag atatgcggag
     1981 cgggggcttt tcaaaacggc atgcgtgccg aaaaaatcag accatggtta tagctgtggt
     2041 aagtggacgt cgccatctgc attccggcgc ccagagttac gccaacgcgg cgatgcagcg
     2101 gccaccggga cacgattccc ggagtaacaa aattttgtat cttcccatca ttcgggccgc
     2161 ctttgaaaaa ggtgctgttg aactcaactt ccggccacca gtgcggattc gctttccact
     2221 gcatcgccgt gttccaggca atcggtcggc ccagtttcct gctgttggcc accggtagcg
     2281 tcgcccccag ggtggattgc acatcgaagc ggccgaatcc ctttcctccc gccaatgtag
     2341 gcgtgaccgt tgcatccgtg ctgccattgc tgtagcttcc cgttggaatg ctgcctccaa
     2401 gaaaggcagt gacgatggcg ttatggcctt cctcattgct cgcataaaag cgatacttca
     2461 tcacaaagga agcatcgcca aagccgtctt tggccttgct gttgtgctga atatagggag
     2521 gcacattcag cagaagttca atcctccgcg tcggaatcag ctccagacct ttgccacctc
     2581 cgtaattcca tgtgtccgtg tgcgttggcg tgatctgccg cataaagtcg gtgcgaaact
     2641 cctgctcaag gcgcggcgtt accgtcgcca gcggagtgac ccagtgcggc tgctcggcct
     2701 gtgtgcggtt cacccgctcc tgccagcggt ttacaaaaga ctgctgtgcg ttggcgggaa
     2761 aaatcgtgca ccctgccagc aacgcaaaga aaattctctt atgaaagcgg tgcaaatacg
     2821 gtctcctcca gtaagctata gcgattttat atgaatcgaa ccgttattta tctccaattt
     2881 atatatgttt tcattggcca ataacccaat tccgctgtcc ggggccgtgc cctacacatt
     2941 tcgcccgcag agtggcatgc tggaagcaat gaatcctgcg ccctgggcgc gtatccaacc
     3001 gtaaaaaggt cagaccacca aaaccgcgac aaaggacaac cgcatgtcta aaccgcgcga
     3061 ccctgaggac tccaaaccgt tgttcggaaa atttacccgg cgctccttcc ttactcagct
     3121 aggcgctgcc ggactcgccg tcaccaccgc tccgctggcg aacgccgctc cggctgctcc
     3181 acaaactgag gccgaaactg ccgctgctga gcctggggcc attccggtca tattgaaagt
     3241 aaatgggcag acccacaaac tcatgctgga gccacgcgtt accctgctgg atgctctgag
     3301 agaggacctc cggcttccag gcacaaagaa gggttgcgac catggccagt gcggtgcctg
     3361 tactgtccac gtgaatggac gccgtgtgaa ctcctgcctg agttttgccg tcatgcacca
     3421 gggcgacgag atcaccacag tcgaagggct tgcacagaat ggagatctgc accccatcca
     3481 ggcggccttt attgagcacg atggctacca gtgcggatat tgcacttccg gccagatcat
     3541 gtccgctgta gcgctgttga aagagccaat cggcagggag gacaacgatg tgcgcgaggc
     3601 gatgtccggc aacatctgcc gttgtggggc ctataacaac atcgtcgccg ccgtgcagca
     3661 ggtgcggcgc tctgcctgat cgagaggaga agacgcaatg cagaccttcc aatatagtca
     3721 ggccaaatcc ccggaagcgg ccgtctcggt tgcaggccag cagacgcggt ttattgccgg
     3781 cggtaccacc ctcgtagacc tgatgaagct gaacgtggaa agacctgctt cggtcattga
     3841 catcaacggc cttccgctcg accgcatcga acccacctcc gatggcggcc tgaggatcgg
     3901 tgcactggtg cgcaacagcg accttgcctg gaacgccgag gtgaaggagc gctatccggt
     3961 gctttctgaa gcgctgcttt ccggggcctc tcctcaactg cgaaacatgg ccacgacggg
     4021 cggcaatctg ttgcaacgca ctcgctgcta ttactttcgt gacacgaatt acaagtgcaa
     4081 caagcgcgaa cctggctccg gctgctctgc gatagacggc ttccaccgca tccacgccat
     4141 tctgggcacg agcgagcact gtatcgccac ccatccttct gacatggccg tagcgatgat
     4201 ggcgctcgaa gccacggtcc acacacgcgg cgcaaaaggt gagaggagca ttccactgaa
     4261 cgagttttat ctggtcccgg gtacgacccc ggagaaagag aatgtgctcg aaccgggcga
     4321 actcatcacc tacgtcacgc ttccggcgct gccgcatggc acaaagtcac attatctgaa
     4381 gttgcgcgac cgtgcttcct atgagtttgc gctggcatcg gctgcggtcg tcatccagac
     4441 cagcggagga aagatccagt tcgcgcgcgt ggcgctcggc ggagttggaa caaagccgtg
     4501 gcgctccaaa gaggcagagc aggagctgca cggacgcgag gcgaacgagc gcaccttccg
     4561 cgcagcggca gaagcagcgc tgaaaccggc aaagcccctg cgcgacaatg cattcaaagt
     4621 agagctggca aagcgcgtga tcgtccgcgc ccttcagaca acaacccaga cagcatagtg
     4681 gggcagaaga catgagcaca tctatcatcg gatcagcagt tggcagggtt gacggcaggc
     4741 tcaaggtcac cggagcagcg cgttacgccg tggaccatcc gctggaaaac atcgcctatg
     4801 gcgcaggcgt tttcagcaca attgcgaatg gaaagattgt ccgcattgat acgtcagagg
     4861 ccgaaaagat gcccggcgtg attgccattc tgtaccacgg caattctgaa cctatctatc
     4921 gcccggccgg gcagtttgag cacagccggg ccagcgaagc acgcccgccc tttgaagatg
     4981 accaggtcta ttactatggc caatttgtag ctcttgtggt tgccaatacc tttgaacagg
     5041 cgcaggatgc ggcttcgcgg gtacgcgtca cgtatgccga agaaaagccg gtggtgcatc
     5101 tggaagacgc gcccacagga gaacaggtcg ccaactactc gcgtggcaat gcagagagcg
     5161 cgtttgagag tgctcccgtg cagattgacg aaacctacat tacgcctgtc gaaacgcaca
     5221 atcccatgga gatgcacggc accatcgctg tgtggaacgg cgataaagtc actctttatg
     5281 agacaacgca gggcgtcgtg aaccaccagc aggtcatgtc agagatgctt ggcgttccgc
     5341 tcgagaacgt gcatgtcatc tcgcctttct gcggctcggg tttcggtgga aagctctttc
     5401 cctggcccca atccatgctt gccgccatcg ccgcgcgccg cgtaaagcgc ccggtcaagc
     5461 tctacgtgcc gcgctcgatg atgttcacta ccgtaggcca tcgtccttat acgaagcagc
     5521 gcatgaggct cggcgcaacc agggatggca agctcgtttc catccagcac gatgtgcttt
     5581 cgaccacatc caaagtagac acctacgtag agcactgcac atcgtccacc ccgatgctct
     5641 atagctgccc gaatgtcaaa gccgtgcagc atctggtccc gttgcatgtc ggcacaccga
     5701 caccgatgcg cggccccggc acggttcccg gccttttccc gctcgagtcc gccatggacg
     5761 agcttgccat caagctgaac atggacccgc tggagctgcg tctgcgcaac tatgctgaaa
     5821 aggacgaggg ccaggacctg cctttctccg gcaagcacct gcgcgaggcc tatcagacag
     5881 gagcagagcg cttcggatgg agcaggcgca atccgaaggt cgcctccatg cgcaatggtg
     5941 atctggtcct gggctggggc atgtccacgg caacgtggca cgcaggacgc ggtggcgcaa
     6001 ctgtgcgcgt gcgcctgaat gccgacggca ctgcccgcgc atccagtgcc acgcaggaca
     6061 ttggcaccgg gacctacacc gtcatcgcgc aggtggtcgc cgacaagacc ggaatccctc
     6121 tggacaaaat tgaagtcgtg ctcggcgaca gctcgctgac tcccggccca atgtccggcg
     6181 gctctacggc aacagcttcc gtgatgcccg ccgtcgccgg cgcgacgaac aatgctgtca
     6241 acacgctgat ccagattgcg cagcgcacgc ccggctcacc cttttatgta aaggaaactc
     6301 cgggcggcga tcctcccaca ctgaagatga caaacgggta cattcacgcg gcagacaaag
     6361 cgccggacag cggtgtgccc tttcatgaag tcctggccat gcggcggctc agcggtattg
     6421 atggcgaggc gaagaccggc cctgcggaag gcatgcacaa attttccggc cacagcttcg
     6481 gctcgcactt ctgcgaagtc acatgggacc caggcatcgc gcgtctgcgc atctcccgct
     6541 gggtcacagt tatggatgcg ggccgcatca tcaataagaa gacggccacc aaccagatcc
     6601 tgggcggcgt ggtgatgggc atcggcatgg cgctgtttga agagacgctc tacgatccgc
     6661 gcaacggcaa gccgctcaac aacaacttcg ctgattacct ggtctccacc aatgccgaca
     6721 ttccgcagat ggagtgtatc ttcatcgaat atcctgacct gaacctgaac gaatacggcg
     6781 ctcgcggcgt gggcgaaatt ggcctggccg gcgtggcttc cgcgattacg atggcggttt
     6841 atcacgcgac aggggtgcgc gtgcgcaagc tgcccgtgcg tattgaagat ttactggggc
     6901 atgtgtaggg ccgggcacct ttctggtcaa tcgcgattat ccatgtccga gctgaaacaa
     6961 atcgtctccc tctggcgtac cgcgaaggcg cgcagagagg aggtctgcct ggcgacaatt
     7021 gtgcgcacgg aaggcccttc ctatcgcaaa gccggcacac acatgctgct catccgcggt
     7081 gggcaacgcg ccggcaccat cagcggggga tgccttgaag gcgaagtctc gaagaaagcc
     7141 tggtggctca cggaaaaagg ccccaccgta cagcggtaca gcagcttttt cgatgacgat
     7201 tctcccttgc cctacggctc cgggtgcgga ggaacggtct acattctgct ggaccgcggc
     7261 cccgcagccc acgccattct ggatgcgctc gaatccagcc tgacaaaacg catgggattt
     7321 gtagccatca tagatacaaa tcaaggaaaa actctgcttg tccagcaaag caatggcgcg
     7381 gtcttgttcg gggatcagtc cggcgtcggg cagctagcag aacaggcgtt ccgcgagcgc
     7441 cgctcgcttt atgctccgga tgctccggag atcttcgtcg aatacactgc gcctccggct
     7501 gcgctgacca tcttcggcgc gggcgatgac gccaagcccc tgtctgagtt cgctgaacgc
     7561 atcggatggc atgttgttat cgccgatggc cgctcgcatc tcgcccgaaa agagcgtttt
     7621 cccgatgcag accgcgtgca cgtgcttgat tacgaatcac cggcaccgct cagcacactg
     7681 gacctcgacc cggaacacgc ctttgtcctg atgacccaca gctacgagca ggaccgcgct
     7741 cttttgcgcg agctgctgcc gctcgcgccc aaatacctcg gcgtcctcgg gccccgtgcg
     7801 cgcaccgaac gcatcgtctg cgaaattgcg gatgcggtcc atctctcgcc ggaagagtgc
     7861 ctggcccggc tgcattctcc tgttgggttt gacatcggcg cgcactcgcc ggagggcatt
     7921 gcgcttgcca tcgctgctga aatcttcgcg tccctgcagg aacgcgggcc tgcaaaccag
     7981 aagtaccatc atcttcttca tgtcagtaaa tgactgcgcc gccatcgtgc t
//
LOCUS       DASRBV010000073        21493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_208967, whole genome shotgun sequence.
ACCESSION   DASRBV010000073 DASRBV010000000
VERSION     DASRBV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208967"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFA02_02975"
     CDS             complement(<1..971)
                     /locus_tag="VFA02_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08841.1"
                     /translation="MKRSASILLVLVLAWVFPALGEGANKRLILKDGSYQVVTDYQVV
                     GDRVRYKSAERGGEWEEVPKELVDWPATEKWNKEHSPEALAAQEAAAEAAAQNDAAAI
                     DKEAAADRANEQARMPVVSPGLRLPDESGVWVLDTFRGTPELVRLEQSNGDINKNLGH
                     NVLKAAINPMGGTKQLVQIEGARSKIQLHVSQPELYVSLDAGDDNAEAPDDALKIDTH
                     GASSKKDPHNYSSPASRYVIVRVQSRRNLRVVAAMNISMIGKVSHSENIVETNSQILP
                     GGHWMKVVPKEPLSFGEYALMELLSPEEVNLDVWDFGVDPTAPENKNT"
     gene            1112..2758
                     /locus_tag="VFA02_02980"
     CDS             1112..2758
                     /locus_tag="VFA02_02980"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZU08842.1"
                     /translation="MSAKYIFVTGGVVSSLGKGLAAASIGCLLEARGLKVNLMKFDPY
                     LNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHAKLTRDNNLTTGRIYEQIITK
                     ERRGDYLGKTVQVIPHVTNEIKNAMRKVAADCDVALVEIGGTVGDIESLPFLEAIRQM
                     RQELGRENTVFVHVTLVPWISAAQELKTKPTQHSVKEMLSIGIQPDILLCRSDRFLSR
                     EIKSKIALFCNLEEQAVITAKDVDSIYEVPLCFAQENVDALALRYLHIEAPEPDLTRW
                     KDLVYRCYHPKDEVSIGIVGKYVEYEDSYKSLKEALVHGSLAHNLKLRVTWIEAEGLE
                     GPNYEEQLKGFDGILVPGGFGKRGIEGMLNAIRYARENKVPYFGICLGMQTACIEFAR
                     NVCGLAEANSSEFDPATPHRVIYKLRELIGVEEMGGTMRLGAWTCILQPDSLAYKAYG
                     STEISERHRHRYEFNREYEALLTGGGLRITGTTPDATYVEIVEIPGHPFFLGCQFHPE
                     FKSKPLEPHPLFSAFVKASYENRMSGATQEQTEEERVLKS"
     gene            2825..3490
                     /locus_tag="VFA02_02985"
     CDS             2825..3490
                     /locus_tag="VFA02_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HZU08843.1"
                     /translation="MRIHSILDPVEYEHRTSIPAILLTLLIGLVLGAAALVWFVRHPK
                     NGFWNEMAARITGRSLALDSSLPTVVSKIQQLQRLETVVYTMDKVEEGSRESSYLPNF
                     LAGDKLLLVVHGQTVAGIDLSQLKASDVQIDGRFVRVHLPQPEVFSTTIDNTKTRVYS
                     RETGLLVGADPDLETEVRAKAQQDLQQAALDDGILEAAHKNAAATVTTMLLGLGFEQV
                     QVQ"
     gene            complement(3498..4733)
                     /locus_tag="VFA02_02990"
     CDS             complement(3498..4733)
                     /locus_tag="VFA02_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZU08844.1"
                     /translation="MAQSTHPVPPAIAALPNLSSQARPFTNDERLARIERAKELMAAN
                     KIDAVLLANHSTSSEYFANVRLDGSERLWALALTAKGRPFLVCPAFEEDRARDLLTAG
                     PFGKDADILTWQEDEDPFALTIQGLKDRGIATGTVGLDENMKYIFSSSLQKAGPQIQW
                     TSAVPVTAGCRMIKSQHELDCMRLACRATLLVYRAVYQSLQPGMTTRDVEKLIEAAYQ
                     KVGFEGEASLNIGEYTALPHGSDQVQTIREGTILMLDDGCSVEGHWSDITRTFVLGNP
                     TDKMKRVFEIVHSAQSTALAAAKPGVAAADVDAAARRVIVDAGFGPGFKYFTHRLGHG
                     IGMDMHEWPYFVKNNMFGWELEPKLQPGMVLSDEPGIYIRGEFGVRLEDDLHVTENGA
                     ELFTPQSPSLEDPFAGFAG"
     gene            complement(4843..4918)
                     /locus_tag="VFA02_02995"
     tRNA            complement(4843..4918)
                     /locus_tag="VFA02_02995"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4883..4885),aa:Ala,seq:cgc)
     gene            complement(4985..5059)
                     /locus_tag="VFA02_03000"
     tRNA            complement(4985..5059)
                     /locus_tag="VFA02_03000"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5025..5027),aa:Cys,seq:gca)
     gene            complement(5130..5204)
                     /locus_tag="VFA02_03005"
     tRNA            complement(5130..5204)
                     /locus_tag="VFA02_03005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5170..5172),aa:Gly,seq:gcc)
     gene            complement(5244..6509)
                     /gene="fucP"
                     /locus_tag="VFA02_03010"
     CDS             complement(5244..6509)
                     /gene="fucP"
                     /locus_tag="VFA02_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HZU08845.1"
                     /translation="MGVTRVSSSSTPQTGGPLTERRFIAPLMLITSLFFLWAVGVNLN
                     DILIPHLKGAFHLTDFQSSFVQVAFFGGYCLAAWPAGRLMERIGYQRGIVCGLIICAT
                     GAFLFIPAAAVRMYWVFLIALFIMACGQSFLEVSSNPYVTFLGPPESSERRLNLAQSF
                     NAVGAVVTPIFGSALILSRAHSAAASETDVVRLPYLLIVGIFLVMALIFWSAKLPVIQ
                     EEKAEEESPRFSGILAFPHLLKGVAAQFCYVGAQVGVASFVIRYVQHTHPGTLDTTAA
                     NYLKLHLVGFMIGRFTGSGLMKKIKPAHMLAAFAVGCIASVIPVIFLHGILSLAGIVL
                     LGFFHSIMFPTIFALSIRHLGPYTKRGSSLEVMAILGGAVFPALMGKISDLSNIQSAF
                     FVPFACYLVVLYFAVSGYKVRTAVMEREV"
     gene            complement(6519..7367)
                     /locus_tag="VFA02_03015"
     CDS             complement(6519..7367)
                     /locus_tag="VFA02_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU08846.1"
                     /translation="MRFVTFTGSDGNSRPGALVNDAVVDLSSRFRSVLSIILGGAEAF
                     SAAKELATAAASRFSLEEVKLLAPIPDPPRIFCIGLNYRDHAIESKMEIPKVPTVFLK
                     LPSALIGDREAIQLPSTSAQPDYEVEFACVIGKDGFRIRREDWQKYVFGYTILNDVSA
                     RDVQLATSQWTLGKSFNTFAPLGPVIVTKDEIADPHALDIRLSIDGEVLQHSNTRELI
                     FKLPDLIAYISSIVPLLPGDIISTGTPAGVGLGRTPQRWLKPGETVTCEVAGLGQLVN
                     PVVAGE"
     gene            complement(7371..8669)
                     /locus_tag="VFA02_03020"
     CDS             complement(7371..8669)
                     /locus_tag="VFA02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZU08847.1"
                     /translation="MSSDVKIINARVIDLRFPTSRDHIGSDAVNVDPDYSAAYCILET
                     DHGPSGYGLTFTLGRGTELCVEALKYLSRFVVGRPLSALTDDLGAFYRQLTSDSQFRW
                     LGPEKGVIHLATGALINAVWDLYARVEGKPLWKLLADMDSEEIVRAIDFRYITDALTP
                     EEAKQMLCERKTGMDARVKQLQQTGYPAYTTSVGWFGFSDEKIEHLCDKALAEGWTHF
                     KLKVGGRPEDDLRRARLVRSKIGPDRRLLMDANQKWDVAEAIERTRALAEVNPWWMEE
                     PTSPDDILGHARIRREVPNVRIATGEHCHNRVMFKQLMQAQAIDVCQIDSCRLAGVNE
                     NLAVILLAAKFNVPVCPHAGGVGLCEYVQHLSVFDYLRVSNTLEDRVTEFVDHLHEHF
                     TDPVRIRNAHYVLPEKPGYSAEIYPETLRDYAYPTGRVWS"
     gene            complement(8688..9446)
                     /locus_tag="VFA02_03025"
     CDS             complement(8688..9446)
                     /locus_tag="VFA02_03025"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU08848.1"
                     /translation="MNEGKEAFRLDGKYALVTGGASGIGEATCRELARAGAFVTVADI
                     NLEKAQQLAGTLPNAKAALMDVTKRASIESVAAQLEKLDILVNNAGIAHVGDIAHVEE
                     ADFDRLMEVNVKSVYLVTKAMFPLLLKSRGSIVNIASVAGLVGVKQRFAYCTTKGAVV
                     AMTRQLAVDYPKELRVNCICPGTVYTPFVEGYLDKYHAHEKDKMIAELNARQPMGRMG
                     KPEEIASMVRYICSDEAEFMTGSALTIDGGWTAA"
     gene            complement(9443..10678)
                     /locus_tag="VFA02_03030"
     CDS             complement(9443..10678)
                     /locus_tag="VFA02_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZU08849.1"
                     /translation="MPQIRLRIAVREFSDFENALAEEIDLYRASHPDVEFEPVPLDLH
                     KLHAELFEKHGLRNGTWDIGYITTDWLAEAAEEGALEELTPYMQRKPVLDWPQGWARS
                     IVDPLYFGDKLYCFPWHDGPECLIYRRDLLEDPKEQQAFRNKYGYELKPPVTWRQFGD
                     IAHFFTRPEQNLYGTLFAAFPDGHNTLYDFALQLWSRGGEFEDKDGNALLDTREAISA
                     LDFYRNTICDASMCYPDAEKYDSTRSGDVFLSGQVAMMVNWFGFAARCDRPGSPLRGK
                     VAIAPIPAEEGKPAASLSVFWTLGIGTGSKHKQAAYDFIHFLMQPELDLGIVKHGTVG
                     VRLSTWRNHEVQKQAPAYSRIEEVSLGARRLPRSRNLPAFAGILNDVAMEALTTQTPS
                     AEILKKAQRRITEMGINFR"
     gene            complement(10682..11764)
                     /locus_tag="VFA02_03035"
     CDS             complement(10682..11764)
                     /locus_tag="VFA02_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU08850.1"
                     /translation="MDMHFDLRQQALHGAMPPLPKKPRPVVVIGAGSIVHDAHLPAYR
                     KAGFPVAALVDANQERAASVAQKFAVPLATTSITEAISKSPQDSVFDVAVPAGAVLDV
                     LPHLPDGAAVLIQKPMGNTLEEAKQILALSRKKGLTAAVNFQLRYAPVMLAAKRICDA
                     GLLGEIHDMEVVVSVYMPWDLWTFLATAPRLEILYHSIHYVDLVRSWFGNPERVLAKT
                     VRNPLTAKLAATKSVITLDYGEWKRVYIATNHGHQYQDSQQSYVQWEGTEGALRAVMG
                     VNLDYPKGKPDSLSMAGRGEAWQTLQTEGNWFPDAFIGTMGSLQAYICGETNTLPTSV
                     EDAIDTMRTVEAAYISSERDGVPLPR"
     gene            complement(11835..13409)
                     /locus_tag="VFA02_03040"
     CDS             complement(11835..13409)
                     /locus_tag="VFA02_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZU08851.1"
                     /translation="MKISRPAGWLASALVALSATAFAQQPNQDDLIWQKSVSKFDKTR
                     QAFLSTVDRAAHEGPFQPDWDSLKGYKIPEWYQDAKFGIFIHWGLYSVPAFGNEWYPR
                     NMYRQGTPEFRHQMETYGPQTKFGYKDFIPMFHAENFDPKAWAALFKELGAQYVIPVA
                     EHHDGFQMYKSDLSDWNAAKMGPKRDIIGELRSAILAEGLHFGASSHRAEHYWFMNGG
                     REFPSDVQDPRYASFYGPAHVGIDPEKNGTGHPDKAYLDDWLARSAEIVERYHPELVY
                     FDWWVEQDTFQPYLKRFAAFYYNQAAKNGQQVVLYRKNDAFPDGTTVLDIERGQLDHI
                     RPQHWQTDTSVSDKSWGYVKGDTYKSPESLVWQLVDIVSKNGNLLLNIGPKSDGTIPD
                     EAANILRRMGAWLKVNGEAIYGTRPWTIYGEGPTKVVAGSFHDTSTQPYTPQDIRFTT
                     KGKTVYAIALGWPTDGHLTIHSLNTGSGFKVGSVSLLGSNAAIHFEQKDDGLHLDLPS
                     QAPGEYAYSFKLAPSE"
     gene            13558..14361
                     /locus_tag="VFA02_03045"
     CDS             13558..14361
                     /locus_tag="VFA02_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU08852.1"
                     /translation="MKTIKRRYPTPALEKGLDILELFAHEPSGLTKSDVARRLNRTVS
                     EIFRMLLCLEERGYIARSEDDDRFRLTLKMFRMSLEYPPTKRLVSEALPIMQEVVHQL
                     NQSCHLGVLEAGQVVIVAQADSPISPGFYVKAGSIVDLMHAATGHVILAHLPQEARER
                     AITTWQYNTGNRVPKDLDEHLEKIRAQGYEERDSYEIRGVINISYPVLDERGNAVAAL
                     TVPYLARIEGSTTTKQVRSVLRDASVKLSEAIGGVAHPATQGERKAAKK"
     gene            complement(14327..15688)
                     /gene="mtaB"
                     /locus_tag="VFA02_03050"
     CDS             complement(14327..15688)
                     /gene="mtaB"
                     /locus_tag="VFA02_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZU08853.1"
                     /translation="MEGYHVENFGCRASRSDGEAIAANLRHQGLGSAGDFSTASVVVV
                     NTCSVTEEADREARAFIRRVHRRNPQAKILVTGCYAQRAPEEVAAIEGVSAVIGNSHK
                     SLVVSEVLGASGFVPLARLVNNVPVLHDTSFAHTELSVLPFAEDARQTRPNLKVQDGC
                     ANRCSFCIIPETRGPSRSVALAECLASVHRFVENGGQELVLSGINLGRWGRDLGPRQR
                     FADLVAAILERTSLPRLRLSSIEPMDWTPDLLALYAQYAAQGRLARHAHLPLQSGSDT
                     ILRKMHRRYRPWHYAEKLAQIRALMPDAAIGADVMVGFPGETDALFQESYDFIAAQPF
                     TYLHLFPFSARPGTPAAAMHRDHPVPAKAVNERMTALRGLLAEKNRAFRESFLGRELS
                     VVTLENRTGASTSALSDNFIKVEISAALPANRIVTVYISGLGEMGLHAEFSTSSRPSS
                     LPV"
     gene            15768..16232
                     /locus_tag="VFA02_03055"
     CDS             15768..16232
                     /locus_tag="VFA02_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU08854.1"
                     /translation="MKRRILLVDDELAILLTLKAVLEINGFEVETAASAREAKSKIKS
                     GQYHMVITDMRMESDTAGLDVVQAARKAPYKPAIAMLTAFPASGAEIEDGADEVLVKP
                     MNTQDLLVQIEALLVTHEDKKRVRSERDLALAGVAHETGTTKRSSKAAGASK"
     gene            complement(16272..16592)
                     /locus_tag="VFA02_03060"
     CDS             complement(16272..16592)
                     /locus_tag="VFA02_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HZU08855.1"
                     /translation="MDFTDRILKCVDCGNEFVFTAGEQLFFHDKQFKNDPKRCKQCKA
                     RRSAGVSARTETRTVCSECGTETTVPFKPTQGRPVLCRQCFQKKRNPAASGTPAPADR
                     PAAL"
     gene            complement(16828..17025)
                     /gene="rpsU"
                     /locus_tag="VFA02_03065"
     CDS             complement(16828..17025)
                     /gene="rpsU"
                     /locus_tag="VFA02_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZU08856.1"
                     /translation="MAEVRVQEGEPLENALRRFKRKVQQEDIIKEVKRHSFYLKPGEK
                     RRMKEALARKRNRKKARKEQD"
     gene            17359..18486
                     /locus_tag="VFA02_03070"
     CDS             17359..18486
                     /locus_tag="VFA02_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZU08857.1"
                     /translation="MTALLATGCGTAVRSSSAGTSGAPVITSASSVQISTGVPFEYQI
                     TATNDPAVYSAQGLPSGLSLNPSTGLISGVPQEQGDFDVVVGAQNASGEGQMHLFLSS
                     GTLTGNCGSSIVPPRAAASAGFTVLSFCDDFDSLSTIDTASTGRAGYNWYTNLSKWSA
                     RSTQPSAYSIAGSVLSLTSTGWTSNYGLSTRDPLTGNGHAWIYGYFEARISFDPTLGT
                     NSQGWPSVWLFSAAHAQSGDPTIWPELDIFEANTGGYAPYSGDYYGTLHQWQDNSIIH
                     YQNSNNRQPTQTDWTRWHTVAVLWTQGEVTWYLDGTAMMTQRYSPTAPPSPLAMTKSG
                     VTPTPPGVFDILDTQAPGMELILGSSPGWPIQVDWVRVWQP"
     gene            complement(18467..19345)
                     /locus_tag="VFA02_03075"
     CDS             complement(18467..19345)
                     /locus_tag="VFA02_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08858.1"
                     /translation="MKESIRGPLVNAACSPLLLRNRFSGFSVVHPESNVVVSLTTHGV
                     RLKTAHVALESIGRGTVKPSRLILWIDKEDAAHGLPSTIQRLQKRGLEVMVSDGKYGP
                     HTKYYPYLKSTQDFTAPLVTADDDIVYPKWWLRDLLEAYHKNPRVVNCHRAHVVVCRN
                     GKIASYSEWQSCTTVEERFENFATGVSGVIYPPEFQRVLFQAGDGFQQCCPWADDVWL
                     HVQALRAGYKVRQVREKPAHFWWVPATGDSALMHRNVLSARPGNDDQIAATYTDADVQ
                     TILTGSVVSTFTAARP"
     gene            complement(19367..20218)
                     /locus_tag="VFA02_03080"
     CDS             complement(19367..20218)
                     /locus_tag="VFA02_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08859.1"
                     /translation="MADLLTRLKRKLRIHLWVARLATRNRTSKRRIVSANNDVIVSLT
                     TYALRIATVYLTIESIAAGDVLPGRIILWLDDAGLYHNPPETLRRLVRRGLEIKLCDN
                     YGPHTKYYGHLASGDPLTSPLVLADDDSLYHKEWLRDLLLAYQQFPEMLNCYLAKPVI
                     VREGKVAPYGEWRLCESTGPSLCNVAHGVAGVIYPPSLLQAVRKSGTSFQSICPKADD
                     IWLHWHALRQRVPTRQIHKTSLRPVFIPGTQDVGLWLQNYQGGNDEQIARTYQPQDIE
                     VLAGCRA"
     gene            complement(20232..>21493)
                     /locus_tag="VFA02_03085"
     CDS             complement(20232..>21493)
                     /locus_tag="VFA02_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZU08860.1"
                     /translation="RIKNSVMARNAGWVMAGTGTGIIMQGFTFILLARLLGPREYGIY
                     VAAFAFAMIASQYSALGSGTVLLRYVSIDRSQFAPYWGSVLLTLLTVSTLLIGGITFL
                     GRKTLKPESASLIIFAGIANCFFQQLAVEAGRVFQAFQKLKLTALVNAVTNTVRLIAV
                     VVLLVIERHSTAWQWSIMSVVVSGIAALLAFGLVTFQVGLPKFSLSNLFGNIGEGFGF
                     AFAGSTTAVYNDLDKTMLGHYGMDRANGIYTMAYRIVDIATTPISSIQAAAIPKFFQR
                     GKESLSAAAELSNTLLLRVVGVGAAVAVAMFLVAPVLPVVLGHGFQDSVEALRWLCWI
                     PIFRGIHLINGCALTGSGLQNYRTAAQVVASVFNFLLNLYLIPVHGWHGAAWSSLMTD
                     GGLALLNALILFFLLKKRERKEALVLH"
BASE COUNT         4826 a         6118 c         5913 g         4636 t
ORIGIN      
        1 gtgttcttgt tctcgggcgc ggtcgggtct acgccgaagt cccagacatc caggttcact
       61 tcttcggggg aaagcagttc catcaaagcg tattcaccaa agctaagcgg ttccttcggg
      121 acgactttca tccagtggcc gccgggaagt atctgtgagt ttgtctcaac aatgttttcc
      181 gaatgagaga ccttcccgat catgctgatg ttcatcgctg ctaccacacg cagattgcgc
      241 cgcgactgca cgcgcacaat cacgtagcgg ctggcgggtg agctgtagtt gtgcgggtct
      301 tttttagagc tggccccgtg cgtgtctatt ttcagggcat cgtcgggagc ttcagcgttg
      361 tcgtccccgg cgtccaggct gacgtatagc tccggctggc ttacgtgtag ctggattttc
      421 gaacgggcgc cttcaatctg gaccagttgt ttcgttccgc ccatcggatt gatggccgcc
      481 ttcagcacat tgtgaccgag atttttgttg atgtcgccat tgctctgttc cagccggacc
      541 agctccggcg ttccacgaaa ggtatccagc acccagacgc cgctttcatc cggcagccgc
      601 agcccgggag aaacgacggg catacgcgcc tgttcgttgg cgcggtctgc ggcagcttct
      661 ttgtctatcg cggcggcatc gttctgcgcc gcggcctcgg cagcggcttc ctgcgcggcc
      721 agcgcttccg gagaatgctc cttgttccac ttctcggtgg ccggccagtc tacaagctct
      781 ttggggacct cctcccattc gcctccgcgc tcggcgctct tgtagcgaac acgatcgcca
      841 accacttggt aatcggtcac aacctgatag gagccgtctt tgagaatgag acgtttgttt
      901 gctccttctc ccagagcagg aaagacccag gcgagcacga gaacaagcaa aatggaggca
      961 gagcgcttca tgctgttgat ttagacgccc ctgtatcccg cagagtcacg gggcgttaag
     1021 aatcatcttt cattctcttt caaatcacgg taaaatcaat cgtgcctacc tggtacgcca
     1081 atcattacaa caggaaaaag gtgaagcagc gatgtccgca aagtacatct ttgtgaccgg
     1141 cggagttgtg tccagcttag ggaagggttt ggcggccgcc tccatcggct gccttctgga
     1201 agcgcgaggg ctcaaggtca acctgatgaa gtttgaccct tacctgaacg tggacccggg
     1261 gacaatgtct cccttccagc acggcgaggt cttcgtcacc gacgatgggg ccgagaccga
     1321 cctcgacctc ggacattacg agcgcttcac ccacgcgaag ctcacgcgcg acaacaatct
     1381 cactactggc cgtatttacg agcaaatcat cactaaagag cgtcgcggcg actacttagg
     1441 caagactgtc caggtcattc cgcatgtcac caatgagatc aagaacgcta tgcgcaaggt
     1501 cgcggctgac tgtgatgttg ctctggtcga gattggcggc acggttggcg acattgagtc
     1561 gctgccgttt cttgaggcca tccgccagat gcgccaggag ctgggccgcg agaatacggt
     1621 gtttgtacac gtcacactcg ttccctggat cagtgccgct caggagctga agaccaaacc
     1681 gacgcagcat tcggtaaagg agatgctgtc catcgggatc cagcccgaca tcctgctctg
     1741 ccgcagcgac cgctttctct ccagggaaat taagagcaag attgcgctct tctgcaatct
     1801 ggaagagcag gccgtcatca ctgcaaaaga cgtagattcc atctacgagg tgccgctttg
     1861 ctttgcccag gaaaatgtgg atgctctggc gctcaggtat ctgcacattg aggcccccga
     1921 gccggacctg acgcgctgga aagaccttgt ttatcgttgc tatcacccga aggacgaagt
     1981 ctccatcggc attgtcggta agtatgttga gtacgaagac agctacaaat ctctgaaaga
     2041 agcgctcgtc cacggctctc tcgcacataa tctgaagctg cgcgttacat ggatcgaggc
     2101 cgaaggactt gaagggccga actacgaaga gcagctcaaa ggctttgacg gcattctcgt
     2161 tcccggagga tttggcaaac gcgggatcga aggtatgttg aacgccatcc gctatgcgcg
     2221 cgaaaacaag gtgccgtatt tcggaatctg cctgggcatg cagaccgcat gcattgagtt
     2281 tgcgcgcaat gtgtgcggtc tggccgaggc caactccagc gaattcgatc cggccacgcc
     2341 gcaccgcgtc atctacaagc tgcgtgaact gattggcgta gaggagatgg gcggcacgat
     2401 gcgcctgggc gcgtggacct gcattctgca accggattcg ttggcataca aagcttacgg
     2461 ttcgacagaa atcagcgagc gccatcgcca tcgctatgag ttcaaccgtg aatatgaggc
     2521 gcttctgacc ggaggcggcc tgcgcatcac cggcaccact cccgatgcca cttatgtgga
     2581 gattgtcgaa atccccggac atcccttctt cctgggctgc cagttccatc ctgaatttaa
     2641 atccaagccg ctggaaccac acccactctt cagcgcgttc gttaaggcga gctatgagaa
     2701 cagaatgagt ggcgctacgc aggagcaaac agaagaagaa cgggtcctga agtcctgagt
     2761 ttggtctggc agatttcaag gcctgccatt catcgggaat cctgcttatg ccatctaagc
     2821 tggcatgaga attcactcca tcctggatcc agtggaatac gagcaccgta ccagcattcc
     2881 agccattctt cttaccctgc tgattggcct tgttctgggt gctgcggcgc tggtctggtt
     2941 tgtgcgtcat ccaaagaatg gcttctggaa tgagatggcc gcacggatca ccggacgcag
     3001 tcttgcgctc gattcatccc tccctaccgt cgtctccaaa atccagcaac tccagcggct
     3061 agagaccgtg gtctatacca tggacaaagt ggaagaaggc tcacgcgaat cctcctatct
     3121 gccgaatttc cttgccggag acaagctgct gcttgtggtg catgggcaga ccgtcgcagg
     3181 aattgatctg agtcaactta aggccagcga tgtgcagata gacggacgct ttgtgcgcgt
     3241 gcatctgccg cagcccgagg tcttttcgac aaccattgac aacacaaaga cgcgtgtcta
     3301 ctcgcgcgag acagggcttc tggtaggtgc tgaccctgac cttgaaaccg aggtacgcgc
     3361 caaagcgcag caggacctgc aacaggcagc gctcgacgac ggtattctgg aagcagccca
     3421 caaaaatgct gccgccacag tcaccaccat gctgctgggt ctgggattcg aacaggtcca
     3481 agtccagtga tcgcgggcta tccggcaaat cctgcaaatg ggtcttcgag cgagggactc
     3541 tgcggcgtaa acagctctgc accgttttcc gtcacgtgca gatcatcttc gagacgtacg
     3601 ccaaactctc cgcgaatgta aatgccaggc tcatctgaca gcaccatgcc cggttgcagc
     3661 ttaggctcca gctcccatcc gaacatgttg ttcttcacaa agtagggcca ctcgtgcatg
     3721 tccataccga tgccgtgccc cagccggtgc gtgaagtact taaatcctgg gccgaatccc
     3781 gcgtccacga tgaccctgcg cgcggccgca tccacgtccg cggcagccac gcccggcttt
     3841 gccgccgcca gcgcagtgga ctgcgcgctg tgcacaatct caaagacgcg cttcatcttg
     3901 tcggttggat tgcccagaac gaatgttcgt gtaatgtctg accagtggcc ctcgaccgag
     3961 caaccgtcgt cgagcatcag gatcgtgcct tcgcggatgg tctgcacctg gtccgatcca
     4021 tgcggcaggg ccgtgtattc accgatattc aggctggctt cgccttcaaa tcccaccttc
     4081 tggtacgctg cctcaatcag cttctctaca tcgcgtgttg tcattcccgg ctggagggac
     4141 tgatacacag cgcggtagac aagcagcgtt gcgcggcacg ccagacgcat gcagtccagt
     4201 tcgtgctggc ttttgatcat gcggcaaccg gcggtcaccg ggacagcgct ggtccattga
     4261 atctgcggtc cggctttctg gaggctgctt gagaaaatat atttcatatt ttcgtcgagg
     4321 ccgaccgtgc cggttgcaat gcctcggtct ttcaggcctt ggatggtcag cgcgaacggg
     4381 tcctcgtctt cctgccacgt cagaatgtcg gcatctttac caaatggccc tgccgtaaga
     4441 agatcgcgtg cgcggtcctc ctcaaatgcc ggacaaacca gaaatggtct gcccttcgct
     4501 gtcagggcca acgcccacag gcgctcgctt ccatccagcc ggacattggc aaaatactct
     4561 gaagaagtgg aatgattcgc cagcagaacg gcatctattt tgttggccgc catcagctct
     4621 ttggcccgct cgatgcgggc gaggcgctcg tcatttgtaa acggtcttgc ctgtgaagaa
     4681 agattgggca gcgcagcaat ggcaggtggt acgggatgcg tactctgggc catcaaaggc
     4741 cggcttagtc caaaaacggc agcagccgaa gaaagatgaa agaacttccg gcgggaaaac
     4801 atggctccat gctacacaaa gtgctctgga ttcgggtctt tttggtggag ccaggcggga
     4861 tcgaaccgcc gacctcctcg ttgcgaacga ggcgctctcc cagctgagct atggccccga
     4921 ataggtgggt gagaactgct tttagtgtaa cagcgcagtg acgattcgca aagttacagc
     4981 caattggagg cgcgggtcgg aatcgaaccg acgcataaag gttttgcaga cctctccctt
     5041 accacttggg taccgcgccc tggacaggaa atcttacttc agaaggcgac tgtgaagcca
     5101 gtctgcgcgg agttttttct tcggctgact ggagcgggag acgggatttg aacccgcgac
     5161 cctcgccttg gcaaggcgat gctctaccac tgagctactc ccgcaggatt actttttgag
     5221 tatatcggtt gagggaagta cggtcaaacc tcgcgctcca taactgccgt ccttaccttg
     5281 tatccggaca cggcaaaata cagcacaacc agatagcagg caaacggaac aaagaacgcg
     5341 ctttgaatgt ttgacaggtc cgaaattttt cccatgagag cagggaagac tgcaccgccg
     5401 agaatggcca tcacctcgag cgaagaacca cgtttggtgt aggggccaag atgccggatg
     5461 ctcaacgcaa agatggtcgg gaacatgatg gagtggaaga agcccaggag tacgattccg
     5521 gcaagcgaca agataccatg caggaaaatt accggtatga ctgaggcaat gcagccaaca
     5581 gcgaaggcgg ccagcatgtg cgccggcttg attttcttca tcagtcccga gccagtgaag
     5641 cgtccaatca taaagccgac aagatgcagc ttgagataat tcgcggcagt cgtgtccagc
     5701 gtgcccggat gtgtgtgctg cacgtagcga atgacgaagc tggccacgcc cacctgcgca
     5761 ccgacatagc agaactgtgc ggccacgcct ttcaacaggt gcggaaaggc caaaatacct
     5821 gaaaagcgcg gtgactcttc ttcagctttt tcttcctgga tcacaggcag ctttgctgac
     5881 cagaagataa gcgccatgac cagaaaaatt ccgacaatca gcagataggg cagacggacg
     5941 acatcggtct ctgatgccgc cgctgaatgg gcgcgcgaaa gaatcagtgc cgaaccaaaa
     6001 atgggagtca caactgctcc cactgcattg aaggactgcg ccaggttcag ccggcgttcg
     6061 gaagactccg gaggcccgag aaaggtgacg taaggattcg aggagacctc aagaaatgac
     6121 tgtccgcagg ccataatgaa caaagcaatg agaaagaccc aatacatgcg gacagccgcc
     6181 gcgggaataa agagaaatgc acctgtcgcg cagatgatca ggccgcaaac gatgccgcgc
     6241 tggtagccga tgcgctccat cagacggccg gcgggccacg cggcaaggca atatccaccg
     6301 aagaaggcca cctgcacgaa ggacgactga aaatctgtaa gatgaaatgc gcccttcagg
     6361 tgcgggatga gaatgtcatt caggttgacg cctaccgccc acaaaaagaa caggctggtg
     6421 atgagcataa gcggcgcaat gaagcgccgc tccgtcagtg ggccgcctgt ttgaggcgtg
     6481 gatgaagatg agacgcgcgt gactcccatg agtgttgatt attctccggc cactacagga
     6541 ttgactaatt gcccaagccc tgcaacttcg cacgtgaccg tttcaccagg cttgagccag
     6601 cgctgcggtg tgcgtcccag gccaacgcct gccggggtcc ctgtgctgat gatatctccg
     6661 ggcaacagag gaacaataga cgagatatac gcaatcaggt cgggcagctt gaagatcagc
     6721 tcgcgcgtgt tcgagtgctg gaggacctcg ccatcaatgg aaagccggat gtccagagca
     6781 tgcggatcag caatttcgtc tttggtgaca atcacgggac caagcggagc gaaggtgttg
     6841 aagctcttac ccaatgtcca ttgtgaagta gcaagctgca catcgcgcgc gcttacgtca
     6901 ttcagaatgg tgtagccgaa gacgtatttc tgccagtctt ccctgcgaat gcgaaagccg
     6961 tccttgccga tgacgcaggc gaactccact tcataatcgg gctgtgctga cgtggaagga
     7021 agctggatgg cctcacggtc gccaatcagt gcagacggca gcttcagaaa cacagtcggg
     7081 accttgggaa tctccatctt cgattcgatg gcgtggtcgc gatagttcaa cccaatgcag
     7141 aagatgcgcg gaggatcagg aatcggtgcc agaagtttta cttcctccag cgagaagcgc
     7201 gaggcagcag ccgtcgccag ttcttttgcc gcgctgaagg cttctgctcc gcccagaata
     7261 atggagagca cgctgcgaaa gcgggaagaa aggtctacga ccgcatcgtt gacaagcgca
     7321 ccgggacggg aattaccgtc gcttccggta aaagtaacga atctcatttt ttaactccag
     7381 acacgtccgg tgggataagc ataatcgcgc agtgtctccg gataaatctc tgcgctgtag
     7441 ccaggttttt ccggcagcac gtaatgagcg ttgcgtatcc tcaccgggtc agtaaaatgt
     7501 tcgtgcagat ggtctacgaa ctccgtcacg cggtcttcca acgtattaga gacacgcaga
     7561 taatcgaaga ccgaaagatg ttgcacatac tcgcacagcc ccacgccgcc tgcgtgcgga
     7621 catacaggaa cattgaactt tgccgcaagc agaatcaccg cgaggttctc gttcactccg
     7681 gcaagacggc aactgtcaat ctggcagaca tcaatggcct gggcctgcat cagttgcttg
     7741 aacatcacgc gattatggca atgttctcca gtggcgatgc gcacattggg cacttcgcgc
     7801 ctgatgcgcg cgtgtccgag aatgtcatcg ggactggttg gctcctccat ccaccacggg
     7861 ttgacttccg ccaatgcccg cgtgcgctcg atggcttccg ccacgtccca tttctggttg
     7921 gcgtccatca ggagcctgcg gtccgggccg atcttgctgc gtactaagcg cgcccggcgc
     7981 aggtcatctt ccgggcgtcc gccgaccttc agtttgaaat gtgtccagcc ttcagccagc
     8041 gccttatcgc atagatgctc gattttctca tcgctgaagc caaaccatcc gacagaagtc
     8101 gtataggccg gatacccggt ctgctgcaat tgtttcaccc gcgcatccat gcccgttttg
     8161 cgctcgcaga gcatctgctt tgcttcctca ggagtaagcg cgtcggtgat gtagcggaaa
     8221 tcaatcgccc gtacaatttc ttccgaatcc atgtccgcaa gcagcttcca gagcggcttg
     8281 ccttctacgc gagcgtagag gtcccagacc gcgttgatga gcgcgccagt ggccagatga
     8341 atgacgccct tctccgggcc gagccagcgg aactggctgt cagaagttag ttggcgataa
     8401 aacgcgccga ggtcgtctgt aagtgctgaa agcggacgtc caacgacaaa gcgcgaaaga
     8461 tatttgagtg cttccacaca aagctctgtc ccgcgcccca acgtaaaggt aagcccgtac
     8521 ccacttggtc cgtggtctgt ttccagaatg caataggccg ccgaatagtc ggggtctacg
     8581 ttgaccgcgt ccgagccaat gtggtcccgc gatgtaggaa agcgcaggtc aatcacgcgg
     8641 gcgttgatga tcttcacatc actactcaat gaaatggttc tccttactta agcagcggtc
     8701 cacccgccat ctatcgtcag cgcagagccg gtcatgaact cggcttcatc tgaacagatg
     8761 tagcgcacca tggaagcaat ctcttccggt ttgcccatgc gccccatcgg ctgccgcgca
     8821 ttcagttcgg caatcatctt gtctttctca tgtgcgtgat acttgtcgag ataaccttcc
     8881 acaaacggcg tatacaccgt gcccggacag atgcagttca cgcgcagttc tttgggataa
     8941 tccaccgcga gctggcgcgt catggccaca accgcgccct tcgttgtgca gtaggcaaag
     9001 cgctgcttca cgcccaccag tccagcaaca gatgcgatgt tcacaatcga gccccggctt
     9061 ttcaacagca gcggaaacat tgccttcgtg accagataga ccgacttcac gttgacttcc
     9121 atcagccggt caaagtcggc ttcttctacg tgcgcaatgt cgcccacatg cgcaatgccc
     9181 gcgttgttta ccagaatatc gagcttctca agttgtgcgg ccacggactc aatggatgcg
     9241 cgtttggtta catccatcag cgcggccttt gcatttggca gtgtgccggc aagttgctgt
     9301 gctttttcaa gattgatatc agcaacagtt acaaatgctc cggcgcgggc cagctcccgg
     9361 catgtggcct cgccaatgcc gctggcgcct ccggtgacga gcgcgtattt tccgtccaga
     9421 cgaaatgctt cttttccttc attcatcgaa agttaattcc catttctgtg atgcgtcgct
     9481 gcgctttctt caggatttct gcggatggcg tttgtgtcgt cagcgcttcc atggcgacat
     9541 cgttcagaat tccggcgaag gccggcagat tgcggcttcg cggaagcctg cgcgcgccga
     9601 gcgaaacttc ttcgattctg ctgtaagcag gtgcctgctt ctgtacttca tgattacgcc
     9661 acgtggaaag ccgcacaccg actgtgccgt gcttcacaat gcccaggtca agttctggct
     9721 gcatcagaaa gtggatgaag tcataggcgg cctgcttgtg cttggatcct gtgccgatcc
     9781 ctagcgtcca gaagacagaa agcgatgcgg caggtttgcc ttcttccgcc ggaatcggcg
     9841 caatcgcaac cttgccccgc aatggtgagc cggggcggtc gcagcgcgcg gcaaagccaa
     9901 accagttgac catcatggcc acttgcccgg aaagaaagac atcgcccgac cgcgtagaat
     9961 catacttctc agcgtccggg tagcacattg aagcatcgca aatcgtgttg cggtagaaat
    10021 cgagcgcaga gatggcttcg cgtgtatcaa gaagcgcgtt gccatccttg tcttcaaatt
    10081 ctccaccccg gctccataac tggagcgcga aatcatacag cgtgttgtgc ccgtccggga
    10141 atgcggcgaa aagtgtccca tataaattct gttcaggacg tgtgaagaag tgggcaatat
    10201 cgccgaactg cctccaggtg accggcggct tcaattcgta accgtatttg ttacgaaaag
    10261 cctgttgctc tttcgggtct tccaaaaggt cgcgacggta aataaggcac tctggcccat
    10321 catgccaggg aaaacagtac agcttgtctc caaagtagag tggatccaca atcgagcgtg
    10381 cccatccctg tggccagtcg aggaccggct tccgctgcat gtaaggcgtc agctcttcca
    10441 gtgcaccttc ctctgcggct tcggccagcc agtcagtggt aatatagccg atgtcccaag
    10501 ttccattgcg aagaccatgc ttctcaaaca attctgcatg tagcttgtgc agatcaagag
    10561 gcaccggctc aaattccaca tccggatgcg acgcgcgata gaggtcaatc tcttcggcaa
    10621 gcgcattttc aaaatcagaa aactcacgaa cggcaatgcg tagacgtatt tgcggcatta
    10681 ttcacctcgg gagcggaact ccgtcgcgtt cactggaaat gtatgcggcc tcgaccgtgc
    10741 gcatcgtgtc tatggcatct tctacgctcg tgggcagagt attcgtttct ccgcagatat
    10801 aggcctgcaa cgaccccatg gtgccaataa atgcgtcagg gaaccagttg ccttctgtct
    10861 gcaatgtttg ccatgcttca ccgcgtccgg ccatgctgag tgaatcaggc ttccccttgg
    10921 ggtaatcaag gttcacgccc atgacggcgc gcagcgcgcc ttccgtgcct tcccactgca
    10981 cataactctg ctgcgagtcc tgatactgat gtccgtggtt tgtggcgatg tacacgcgct
    11041 tccactcgcc ataatcgagt gtaatgacgc ttttggtggc ggcgagcttt gccgtcaggg
    11101 gattgcgcac cgtctttgcc agcacccgct caggatttcc aaaccatgag cggacaaggt
    11161 ctacataatg aatgctgtgg tacaggattt ccaggcgcgg agcggtcgct agaaatgtcc
    11221 acaaatccca cggcatataa acacttacga ccacttccat gtcatggatt tcgcccagca
    11281 gcccggcatc acagattcgc ttcgctgcca gcatgaccgg agcatagcgt aactgaaagt
    11341 tcactgccgc ggtcagtccc ttcttgcgag acagcgccag aatctgcttg gcctcttcca
    11401 gagtatttcc catcggtttc tggatcagca cggccgcgcc atctggcaga tgcggcaata
    11461 cgtcgaggac cgcgcccgcc gggaccgcca catcaaagac gctgtcctgt ggtgatttgc
    11521 tgatggcctc cgtgatcgaa gttgtggcca gaggcacagc aaacttctgt gctacggatg
    11581 ccgcccgctc ctgatttgca tccaccagcg cagcaacggg aaatcccgcc ttccggtaag
    11641 caggcagatg cgcatcgtgc acaatgctgc ctgctccaat cacaaccacc ggacgtggct
    11701 tcttcggcaa cggtggcatg gcaccgtgca gcgcctgctg ccgtaaatca aaatgcatgt
    11761 ccatcagaaa tatctggtgt tagatttcta caaataaaaa catatgtttg tcaataaaat
    11821 taactggcac cttcctattc agaaggtgcc agcttgaagc tataggcata ttctcccggg
    11881 gcctgtgacg gaagatcgag atgaaggcca tcgtcttttt gctcaaagtg aatggcagca
    11941 ttcgatccca gcagcgagac agaaccaact ttgaatccgc tgcccgtatt cagagaatgg
    12001 atcgtcagat gcccatccgt gggccagccc agagcgatgg catacactgt cttgcctttg
    12061 gtggtgaagc gaatgtcctg cggagtgtac ggctgggtcg aagtgtcatg gaaagagcct
    12121 gcgactacct tcgtaggccc ttctccatag atggtccagg gtcgcgtgcc gtagatggct
    12181 tcgccgttca ccttcagcca cgcgcccatc ctgcgcagaa tgttcgcagc ttcgtccgga
    12241 atcgtgccgt cggacttagg cccgatgttc agcagaagat tgccgttttt gcttacgatg
    12301 tccaccagtt gccatactag gctttccgga gacttatacg tatcaccttt cacatagccc
    12361 caggacttgt cgctcacgga cgtatcggtc tgccagtgct gtggacgaat gtggtccagt
    12421 tgtccgcgct cgatgtcgag gacggttgtg ccatcgggaa aggcatcatt tttgcgatag
    12481 agcactacct gctggccatt cttcgcggcc tggttgtagt agaacgccgc aaaacgcttc
    12541 aggtaaggct gaaaagtgtc ctgctccacc caccagtcga aatagaccag ctcaggatga
    12601 tagcgctcga caatctcagc gctgcgcgcg agccagtcgt cgagatatgc tttgtccgga
    12661 tgcccggtgc cgttcttctc cggatcaatg cccacgtgcg ccggtccata aaacgacgcg
    12721 tacctcgggt cctgtacatc agaggggaac tcacgtcctc cattcataaa ccagtagtgc
    12781 tcggcgcgat gcgaagacgc gccaaagtgc aggccttccg ccaggatggc actgcgcagc
    12841 tcgccgatga tgtcgcgttt cggtcccatc tttgcggcat tccagtcact caggtcagat
    12901 ttgtacatct ggaagccatc atggtgctcg gcaacaggaa tcacatactg cgcgcctaac
    12961 tccttgaaaa gagcggccca tgctttggga tcaaagtttt ccgcgtggaa catgggaatg
    13021 aagtctttat agccaaattt ggtttgcggg ccataggttt ccatctggtg cctgaactcc
    13081 ggagtgccct gccggtacat attgcgcggg taccactcgt tgccaaaggc cgggaccgaa
    13141 tacaatcccc agtggatgaa gatgccaaac tttgcgtcct gataccactc aggaatcttg
    13201 tatcccttca gcgagtccca gtcgggttgg aaaggccctt cgtgcgcagc cctgtccaca
    13261 gtggaaagaa atgcctgtcg cgtcttatcg aacttgctta cagatttctg ccagatcaga
    13321 tcatcctgat taggctgctg cgcaaacgct gtagcggata gcgcgaccag tgcggaagcc
    13381 agccatcccg ctgggcggga aatcttcatg tgtatctctc cggaaatgcg aaaaagaaga
    13441 tgccttattt ttatacgcta aaagcagttt tgatgaacaa ttttgttttt gggtccgcgg
    13501 acttgtccgt ttacaatttc tgctaataaa aaaatcctgc tgttcaggaa atgcaagttg
    13561 aaaacaatca agcgacgcta tcccactccg gcgctggaaa aaggtctcga catcctcgaa
    13621 ctgtttgcgc acgagccttc cggcctgacc aagagcgatg tggcccggag gctcaaccgc
    13681 acagtttctg agatattccg tatgctgctc tgtctggaag agcgcggtta tatcgctcgc
    13741 tctgaagatg acgaccgttt ccggctaacg ctgaagatgt ttcgcatgtc gctcgaatat
    13801 cctccaacca agcggctggt ttcagaagcg ctgcccatta tgcaggaagt ggtgcatcaa
    13861 ctgaaccagt cttgccatct cggcgtactt gaagcaggtc aggtggtgat tgtggcgcag
    13921 gctgattcac ccatcagtcc gggcttttac gtaaaggccg gctcgattgt ggacttaatg
    13981 cacgccgcaa cagggcacgt cattctcgcg catcttcccc aggaagcgcg agaacgcgcc
    14041 attacaacct ggcagtacaa cactggaaac cgggtcccca aagacctcga cgagcacctt
    14101 gaaaaaatcc gggcccaggg atacgaggag cgtgacagct atgagattcg cggcgtgatc
    14161 aacatcagct atccggtgct cgacgaacgc ggaaacgccg tggccgcgct gactgttcct
    14221 taccttgcgc ggattgaagg ttccacaaca acaaagcagg tgcgctcggt gctaagggac
    14281 gcgagcgtga agctcagcga agccattggc ggcgttgccc atcctgctac acagggagag
    14341 aggaaggccg cgaagaagta gaaaactctg cgtgcagacc catttcgccc aaccctgaga
    14401 tatacacagt cacaatccgg tttgcgggca gcgctgcgct gatttccacc ttgatgaaat
    14461 tgtcagaaag cgcagatgta ctcgcaccag ttcggttttc cagtgtcacg acgctcagtt
    14521 cgcgcccgag gaacgattca cgaaaggcgc ggttcttttc agccaacaaa ccacggagcg
    14581 cggtcatgcg ctcatttacc gcctttgccg ggacaggatg gtcgcggtgc attgcggccg
    14641 cgggcgttcc cgggcgggcg gagaacggaa acaagtgcag ataagtaaat ggctgcgcgg
    14701 cgatgaagtc gtagctttcc tgaaagagtg cgtctgtttc tccgggaaat ccgaccatta
    14761 catctgcgcc aatggcagca tccggcataa gtgcgcgaat ctgcgcgagc ttttccgcgt
    14821 aatgccaggg gcggtagcgg cggtgcattt tgcgcagaat cgtatctgaa ccggattgca
    14881 ggggcaggtg cgcatgtcgc gcaaggcgtc cctgtgcggc gtactgcgcg tagagcgcca
    14941 gcaggtccgg cgtccagtcc atcggttcaa ttgagctgag gcgcagccgc ggcagggaag
    15001 tccgctccag aatcgcggcg acaagatctg caaaacgttg ccggggaccg aggtcgcgtc
    15061 cccagcggcc caggttgatg ccggagagca ccagctcctg cccgccgttc tcaacaaagc
    15121 gatggacaga ggcaaggcat tccgcaagcg caacagaacg gctggggccg cgtgtctccg
    15181 gaatgatgca gaaggagcag cgattggcgc agccgtcctg cactttaaga ttgggccgcg
    15241 tctgtcgcgc gtcttccgca aacggcagca cagacaattc cgtgtgagca aagctggtat
    15301 catgcagaac aggcacgttg ttgaccagcc gtgccaaggg cacaaagcct gaagctccga
    15361 ggacctcaga aaccacaagg gacttgtggc tgtttccgat gaccgcactc acgccttcga
    15421 tcgcagctac ttcttccggc gcgcgctgtg cgtagcatcc ggtaacaaga atcttcgcct
    15481 gtgggtttct gcgatgcacg cggcggatga aggcgcgcgc ttcgcggtcg gcctcttcgg
    15541 tcacgctgca ggtgttcacg accacaacgc tggcggtgga aaaatcacca gcggaaccga
    15601 gcccctgatg gcgcaaattg gcggcaatgg cctctccatc cgagcggctt gcgcggcacc
    15661 cgaagttctc gacgtgatat ccttccacat gttaagtgta acaaccggac aggagcagtc
    15721 gctggcaggt ccggtgtatg gcgtccccac cattcacagg agtacgtatg aaacggcgaa
    15781 ttttgcttgt ggacgatgaa ctggcgattt tgctgacgct caaggctgtt ctggaaatca
    15841 acggttttga ggtggaaacg gcggcctccg cccgcgaggc aaaatccaaa atcaaaagcg
    15901 gccagtatca catggtcata accgacatgc gcatggaaag cgatacggca ggactggatg
    15961 tcgtgcaggc cgcgcgaaaa gcgccatata agccggccat cgccatgctg accgcatttc
    16021 ctgcgtccgg cgcggagatt gaagacggcg cggacgaagt cctggtaaag ccgatgaata
    16081 cgcaggacct gctcgttcag atcgaggccc ttctggtgac ccatgaagat aaaaagcgtg
    16141 ttcgcagcga gagagatctt gcgctggcag gcgtggcgca tgagacggga acaacaaagc
    16201 gctccagcaa agctgcaggc gccagtaagt aaacccacct tcaggacgat gcagctaaaa
    16261 gagaagtctt tttatagtgc cgcgggccgg tcagcaggag ctggtgttcc ggaagcggcc
    16321 gggttgcgct tcttttgaaa acactgtcgg cacagcacag gacggccctg cgtcggttta
    16381 aagggcaccg ttgtttccgt tccacactcc gagcagacgg tgcgggtttc ggtccgggca
    16441 gacacaccgg cactgcgtct ggccttgcac tgcttgcagc gtttggggtc gtttttaaac
    16501 tgtttatcgt ggaagaagag ctgctcgcct gcggtaaaga caaattcatt gccgcagtct
    16561 acgcacttga ggatcctgtc ggtgaagtcc atattgcgtc tccggcaata agcgcagagc
    16621 ctccgcccgc tctgatggtg ggcgcaggga ctgtttcata acaactacga ctgcttattt
    16681 caaaggggcc cggattgcca gagatataag cacactggct ggcatgctga gaagggcatg
    16741 ccgatgagac atcttacggc tttcttgtaa ttcagcccca ttgcgcgcaa acggcacaat
    16801 ggggcttgat gttctcttaa ggtggagtta gtcctgttct ttgcgggcct tcttgcggtt
    16861 ccgcttgcgc gccagcgctt ccttcatgcg gcgcttttca ccgggcttca ggtagaagga
    16921 atgacgtttt acttccttaa tgatgtcttc ctgctgtacc ttgcgcttaa accggcgcaa
    16981 tgcattttca agcggttcgc cctcctgcac acgaacctct gccaatggaa ctacacctcc
    17041 agacttgccc agaaaggggc ctatatagat taccggattt taccggaaat agcgacataa
    17101 aaccggttct gacggcccaa ccaccctctc tctggagaac gaaaaagcag gcccgcggcg
    17161 ggtcgtaaaa gccaaaacat cggcagcaac ttacgccgtc cgggcctccg ttttgtccac
    17221 gaatgcgcca gtaaagccac cctttcagcg ggccgataac caggctgaag cagagaggga
    17281 cttcagatgg ttttctgtgg acagaaagag agatatagca cacattccca gtctggctgc
    17341 cttgctgcgt gtttcgttgt gacagcgtta cttgcaaccg gatgtggaac ggcggtccgt
    17401 tcgagcagcg ccggaacatc tggtgctcct gtgattacga gcgcatcgag tgtgcaaatc
    17461 agcacgggcg tgccgtttga gtatcagatt accgcaacaa acgacccggc cgtctacagc
    17521 gcgcagggcc tcccctctgg cctgagcctt aatccttcca ccggactcat ttcgggtgtg
    17581 ccacaggaac aaggcgactt tgatgttgtg gttggcgcac aaaatgcatc tggagaaggg
    17641 cagatgcatc tgtttttgtc cagcggcact ctgactggaa actgcgggag ttccattgtt
    17701 ccacccaggg cggcagcttc tgccgggttt accgtacttt ctttttgcga tgacttcgat
    17761 tcgctgtcca cgattgatac agcatccacc ggcagagcgg gatacaactg gtataccaac
    17821 ctctcgaagt ggtctgcccg cagcacccaa ccctccgcct actccatagc aggatctgtg
    17881 ctgtctctta ccagtaccgg atggacgtcg aattacggcc tgtctactcg agaccctctt
    17941 accggaaatg gacacgcgtg gatttacgga tactttgagg cccgtatcag cttcgacccc
    18001 actctgggga ccaactcaca gggatggccg tcagtatggt tgttttccgc cgcgcacgcg
    18061 cagagcggag atccaaccat ctggcctgaa ctggacatct ttgaggcaaa taccggaggc
    18121 tatgctccgt attcgggtga ttattacggc acattgcacc agtggcagga caattccatc
    18181 atccactatc agaacagcaa taaccgccag cccacacaga cagattggac cagatggcat
    18241 acagtggctg tgctctggac gcagggcgag gtgacatggt atctggacgg aacagcgatg
    18301 atgacccagc ggtactctcc gacggcccct cccagccctt tggcgatgac aaagtccggg
    18361 gtcacgccta cacctccagg tgtcttcgac atcctcgaca cgcaagcccc gggaatggaa
    18421 ctgattctgg gcagttctcc cggctggccc atccaggtgg actgggtcag ggtctggcag
    18481 ccgtaaatgt actcacaacc gaaccagtca agatggtttg gacatcggcg tctgtgtagg
    18541 ttgcggcaat ctggtcatca ttgccgggcc gggcagataa aacattccgg tgcatcaacg
    18601 cgctgtctcc tgtagcgggg acccaccaga aatgggccgg tttttcccgg acctggcgga
    18661 ccttataacc ggcccgaagg gcctgtacgt gcagccagac gtcatcggcc cagggacagc
    18721 actgttgaaa tccatcgccg gcctgaaaca gtacgcgctg aaattccgga ggataaatga
    18781 ccccggacac cccggtggca aagttttcga acctttcctc gacggttgtg cagctttgcc
    18841 attcagagta gctcgcaatc tttccattcc ggcagaccac aacatgcgcc cggtgacaat
    18901 tcacgaccct gggatttttg tgatatgcct ccaacaggtc cctgagccac cacttcggat
    18961 aaacaatgtc gtcgtccgcc gtgaccagcg gcgccgtaaa atcctgtgtg gatttcaggt
    19021 agggataata cttcgtatgc gggccatatt tgccgtcgct gaccatgacc tccagcccgc
    19081 gcttctgtaa gcgttgaatt gtggaaggca gtccatgtgc cgcatcttct ttgtcaatcc
    19141 acagaatgag tctggagggc ttcaccgtcc cccttccaat ggattccagt gcaacatgcg
    19201 ccgtcttcag ccggaccccg tgggtcgtta aggagacaac aacgttggac tctggatgga
    19261 cgacagaaaa accggagaag cggtttcgca gcaacaacgg cgaacaggca gcgtttacaa
    19321 gcggtcctcg aattgattcc ttcatgaaac tgttctccgc attaagttat gcccggcaac
    19381 cagcaagtac ctcgatgtcc tgcggctgat aggtgcgggc aatctgttcg tcgttgccgc
    19441 cctgataatt ctgcagccac aagcctacgt cctgcgttcc cggaataaac actggccgca
    19501 gactggtctt gtgtatttgc cgtgtgggta ccctctgccg cagagcatgc caatgcagcc
    19561 agatgtcgtc cgctttgggg cagattgact gaaaagaagt cccgctcttc cggacagctt
    19621 gcagcagaga tggcggataa atgactcctg ccactccatg cgcaacatta cacagtgacg
    19681 gtccggtact ctcacagagc ctccattcac cataaggagc gaccttgccc tcgcgtacga
    19741 tgactggttt ggccagatag caattcagca tttcagggaa ttgttggtaa gccagcagca
    19801 ggtcacggag ccattccttg tggtagagcg aatcatcgtc ggccagcacc aatggacttg
    19861 ttaaaggatc gccggatgca agatgaccat aatacttcgt atgtggccca tagttgtcgc
    19921 acagcttgat ttccagccct cttctgacca ggcggcggag cgtttcaggc ggattgtggt
    19981 atagcccggc atcatccagc cacaggatga tccgtcccgg caagacatcg cctgctgcaa
    20041 tggattcaat cgtgagataa acggtagcaa tgcgcaacgc atacgtcgtc agagagacga
    20101 tgacatcatt gttagcagaa acaatgcgcc gcttcgatgt ccggttgcgc gtagcaagac
    20161 gcgcgaccca gagatggata cgcagctttc gtttcaggcg agtcaggagg tctgccatag
    20221 atatgctcgt gctagtggag gaccagggcc tctttgcgct cccgtttctt caacaagaag
    20281 aaaagtatca acgcattcag cagcgccagg ccaccgtctg tcatcagact cgaccaggcc
    20341 gccccgtgcc acccatgaac gggaatcagg taaagattca gcaggaaatt aaacacgctt
    20401 gcgaccacct gggccgccgt cctgtagttt tgcagacccg agccggtcag cgcgcagcca
    20461 ttgatcagat ggattccgcg gaagattgga atccagcaga gccagcgcag tgcctccacg
    20521 ctgtcctgga agccgtggcc cagcaccacc ggcagcaccg gggcgacaag aaacatggcc
    20581 actgcgacag cagcgcccac accaactaca cggagcaaca aggtgttgga caattcagct
    20641 gcagctgaga gactttcttt tccgcgctga aaaaactttg gaatggcagc ggcctggatg
    20701 gacgaaatcg gcgtcgtcgc aatatcaaca attctgtacg ccatcgtata aatgccattg
    20761 gccctgtcca tcccatagtg gccaagcatg gtcttgtcca gatcgttata gaccgctgtc
    20821 gtggacccgg caaaggcgaa accgaaacct tccccaatat tcccaaagag gttggagagc
    20881 gaaaacttcg gcagcccgac ctgaaacgtt accagaccga aagccagcag ggcggctatg
    20941 ccagagacaa cgacggacat aatgctccac tgccacgccg tcgaatgtct ttcaatcacc
    21001 agcagcacga ccaccgcaat cagcctgacg gtatttgtca cggcattcac cagtgccgtc
    21061 agtttcagct tctggaatgc ctgaaacacc cggcctgcct cgactgcaag ctgctggaaa
    21121 aagcaattgg caatccctgc aaaaataatc agactggcgc tctcaggctt gagcgtcttt
    21181 cttcccagga atgtgattcc gccaatcagc aatgtgctga ccgtcagcag cgtcagcagc
    21241 acgctccccc agtaaggcgc aaattgtgag cggtcaatcg agacataccg cagcagcacg
    21301 gtgccgctgc ccagggcgct gtattgggag gcgatcatgg caaacgcaaa cgcagccacg
    21361 taaatgccgt attccctcgg tcccagcagg cgcgcaagca ggatgaaagt aaatccctgc
    21421 atgatgattc ccgtgccggt gccggccatc acccatccgg cattgcgcgc catcacgctg
    21481 tttttgatcc ttg
//
LOCUS       DASRBV010000074         6116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_459495, whole genome shotgun sequence.
ACCESSION   DASRBV010000074 DASRBV010000000
VERSION     DASRBV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459495"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..739
                     /locus_tag="VFA02_03090"
     CDS             410..739
                     /locus_tag="VFA02_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU08861.1"
                     /translation="MPDTLRRFKAEFFQALAHPTRIAILEQLRDGELSAGAIIEKLGM
                     EQANVSQHLAVLRGKQIVVNRKAGNQVFYSVRDPLIIEVLDLMRKYFHAHLSEALAML
                     EEMEAHR"
     gene            736..2445
                     /gene="sulP"
                     /locus_tag="VFA02_03095"
     CDS             736..2445
                     /gene="sulP"
                     /locus_tag="VFA02_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HZU08862.1"
                     /translation="MKLKDEWLPRSVLCFRNYTLSQFTHDLVAGITVGLVALPLAMAF
                     GIASGVTPQAGMYTAVVAGFLISALGGSRVQIGGPTGAFVVIVAGIVAKFGVSGLSLV
                     TLMAGVLLVIMGATGLGAAVKFIPRPVIIGFTNGIALLIASTQVKDFLGLKTGPVPSD
                     FLKRMHMLIAHLGTTQWQTAAVGCISLAIILLWPRWNRRVPGSILALFAATLAVPLLH
                     LPVETVGSRFGGIPQGFPPLTLPHLRAEQILPLLPSAFTVAMLAAVESLLSAVVADGM
                     IGDKHNSNVELVAQGVANLASPLFGGIPATGAIARTATNIRSGAGTPVAGIIHALTLL
                     AILLVAAPLAKFVPLATLAAVLFVVAWNMGEWREIGSILRLAKTDIAVWLVTFTLTVI
                     ADLTVAVGVGMALAALLYIYRIAETTTVAPVTEEYLRDGQAHILQDKEIPPDVAILRI
                     HGPFLFGTTEKLAEATADLKAFPSVVVLRLRNMTALDATGLHALETFAERLHKSGRTL
                     LLCGAREQPAELLAGSELVRLLGRENILPHVQAALARAREITGAFHGVGPEIAAELNR
                     VPL"
     gene            complement(2513..4369)
                     /locus_tag="VFA02_03100"
     CDS             complement(2513..4369)
                     /locus_tag="VFA02_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08863.1"
                     /translation="MKPITSAMSSLLLLLFVATASAQKKAFEDPGIPLVYNVENTGAH
                     YHAPVFPDFDHLPILRPLPDPFRFMDGWRDTSFQSWERHRNEIKAAIEKYEIGPKPDC
                     SDCTITASYVPPATSSAKGTLTVIVTRKGKSLTLTSGVYIPSGMGSGPFPALIAMTFF
                     ASASGPNYGFLPASVFSSRPVATVDFVHNQVAAYSFSSPSDHTKDPFYQLYPELCAGT
                     CTGTSNSGEYAAWAWGVSRLIDGMEIASHQAVNPLPVDMSHLAVTGCSYAGKMALFAG
                     AFDERIALTIAQENGGGGAPAWRVSREIEPQGSVEDVDDTDYDWFAGTMKQFAGDNVY
                     KLPADHHELMAMIAPRALLETGNTDYYWLSNRSNYISARATQRIYNMLGIGDRFGFYI
                     DGGHVHCGTLPAEAPSIAAFVDRFMLGKAVSTSVEVNPFPTLDYSRWTWWWGRSPLAY
                     FPDNWNTGGSVVMSLHPGWDLLWINSENPVQAGYQFSIPGEHPAATASLTGGNVQMDV
                     LCLDGSSYTLTVPLPAGQSWSIPANSSEWVPGKGVYQGSATAPGCGNMGLPGILVDAY
                     LSALGTSAGVGNPPSAPGFTTSDTADPLRVRFSCSANGDTVQSRPLTVNFQQ"
     gene            complement(4689..>6116)
                     /locus_tag="VFA02_03105"
     CDS             complement(4689..>6116)
                     /locus_tag="VFA02_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZU08864.1"
                     /translation="QSAATLAQIEQACKDKQKELEASLSANKEDTKSLAYQRRREAAN
                     RAVQNLETLRHVLHNWFEFYDGYDPMFTWWAEEPYKTADAALDAYGKFLSEKVVGLHP
                     APEAKNLPPSGEGDEEAYQRSMASAHPGDSSDIIGHAIGRDALIRELNFAMIPYTPEE
                     LIAIAEKEFAWNDNEMMKASREMGFGDDWKKALEKVKNTYVEPGKQTELARKYIFQAL
                     DFLDKHDLITVPPLARETWRMRMIPPQQQLISPFFLGGEVLQVSYPTNTMSYEQRITT
                     MRANNIAMSHATVFHEMLPGHELQGFMEERFRAYRQAFGGTPFLIEGWALYWEMLMWD
                     LGFDRTPEERVGALAWRRHRCGRIIFSLNFHLGKWTPQQCIDLLVDRVGFEKQAAIGE
                     VRRSLGGGYGPLYQAAYLLGGMQIYALHKELVGSGKMTNCQFHDAVLKENSIPVEMIR
                     ASLTNQKLAKDFHTQWKFYGDVKTS"
BASE COUNT         1270 a         1646 c         1760 g         1440 t
ORIGIN      
        1 gtgctttacc aacgcataac catgcatggg cttgaggcgt agcgactgga gaatcatgat
       61 ctccaacgcg ccgggaaaca ggtcgcggtg ttcggacttt ttcgccatga gcgaaagtat
      121 atattcaaaa tacctatttt agaaataggt attttaataa aaataatcca agaggtagtg
      181 ttcagagatt ccaggaacga gagaacattc ttccgcatgt gcaggctgtg ctgccgctcg
      241 cggcgctctt tctgccagac ggttgcgcca ttccatttct tctggcattt cggtgcgcag
      301 actgtatgca gactgtattt tgttgtttcc atgcactgcg gattacagag gagcaagtgt
      361 ttcggcgctc aacatatata tgaataatcg aatatgctaa aatatcctca tgccagacac
      421 cttacgacgc ttcaaggccg aattcttcca ggccctggcc catcccacgc gcattgccat
      481 cctggagcag cttcgcgacg gcgaactttc tgccggggcc atcatcgaaa agctgggcat
      541 ggagcaggcg aatgtctcgc agcatcttgc tgttctgcgg ggtaagcaaa ttgtcgtcaa
      601 ccgcaaagcg ggcaaccagg tgttctattc ggtgcgtgac ccgctgatta tcgaagtgct
      661 cgatctcatg cggaagtatt tccatgcgca cctgagtgag gcactggcca tgctcgaaga
      721 gatggaggcc catcggtgaa attaaaagac gagtggcttc cgcgctctgt tctctgcttt
      781 cgtaattaca cgctttcaca attcacgcat gatctggttg ccggaatcac agtcggcctg
      841 gttgctctcc cgttggcaat ggcttttggg attgcatccg gtgtgactcc gcaggctggc
      901 atgtatacgg cggttgttgc cgggttcctg atttcagccc tgggcggctc gcgtgttcag
      961 attggcggcc cgaccggagc attcgtcgtc attgtggcag gaattgttgc gaaattcggg
     1021 gtctccgggc tttctctggt gacattgatg gctggagtgc tgctcgtgat tatgggcgct
     1081 accggactgg gagcagcggt aaagtttatt ccgcggcctg tcatcatcgg gtttacgaac
     1141 gggattgcgc tgcttatcgc ctccacacag gtgaaggact ttctcggtct gaagaccggg
     1201 ccggtcccca gcgattttct gaagcggatg cacatgctga ttgcacatct gggaacgaca
     1261 cagtggcaga cggccgcagt gggctgcatt tcgctggcga tcattcttct ctggccacgc
     1321 tggaacaggc gagttcccgg ctccatcctt gctttgtttg cggcaacact tgccgtgccg
     1381 ctgctgcatc ttcccgttga aacagtaggc agccgctttg ggggaattcc gcaaggtttt
     1441 ccgcctttaa cactgccaca tctgcgcgca gagcagatac ttccgctgct gccttccgca
     1501 ttcacggtcg ccatgctggc tgcggtagaa agtttgctgt cagcagtggt tgccgatgga
     1561 atgatcgggg acaaacacaa ctcgaatgtt gagctggttg cgcaaggcgt ggcaaacctt
     1621 gcctcaccac tctttggggg cattccggca accggggcca ttgcgcgtac ggcaaccaat
     1681 attcgctcag gcgccggcac gcctgttgct ggaatcatcc atgcgctgac gctactggca
     1741 atcttgcttg tagctgctcc attggcaaag ttcgttccgc tggcaacgtt agcagccgtg
     1801 ctttttgttg tggcgtggaa catgggtgag tggcgcgaaa tcggctccat tctgcggctg
     1861 gccaaaacag acatcgcggt ctggctcgtg accttcacgc ttactgtcat tgctgatctt
     1921 actgtggcag ttggagtagg aatggcgctg gcggccctgc tctacatcta tcgcattgca
     1981 gaaacgacca cggtggcccc ggtgacggag gaatacctgc gcgatggaca ggcgcatatc
     2041 ttgcaggaca aggagattcc gccggatgtg gccatcctgc gtattcatgg cccgttcctg
     2101 tttggcacaa cggaaaagct ggccgaagcc acggcagatt taaaggcatt tccatctgtg
     2161 gtagtcctgc gcctgcgcaa tatgacggcg cttgatgcca ctggtctgca tgctctggag
     2221 acttttgccg aacgcctgca caaatcgggc cgcacgctgc tgctttgcgg tgcgcgcgaa
     2281 cagccagcgg agttactcgc tggatcagaa ttggtcaggc tcctgggacg agagaacatt
     2341 cttccgcatg tgcaggctgc gctggcgcgt gcgcgtgaaa tcaccggggc cttccacgga
     2401 gtcgggccgg aaatcgctgc cgaattaaac cgcgtacctc tataagtgtt gggtgtccta
     2461 catttttttg agatgtagga cacccatttc caaaccgtta aaaatccatc tgtcactgct
     2521 gaaaattcac cgtcagaggt cggctctgga cggtgtctcc attggcgctg caactgaacc
     2581 ggacccggag cgggtctgct gtgtcgctgg tggtaaagcc gggtgctgag ggtggattgc
     2641 ccacgcctgc gctggtccct aaagccgaga gataggcgtc taccagaatg cccggcagac
     2701 ccatattgcc gcatccgggc gctgtagccg aaccctgata cacgcctttc cccggtaccc
     2761 attcgctgct gttggcaggg atggaccagg actgacccgc cggaagcggg accgtcagtg
     2821 tgtaggagct tccgtccaga caaagcacgt ccatctgcac atttccgcct gtcagcgatg
     2881 cagtggccgc cggatgctct cccggaatag agaactgata acccgcctgg acaggatttt
     2941 cagagttgat ccacaagagg tcccagccgg gatgcaaaga catgaccacg gacccgcctg
     3001 tgttccagtt gtcaggaaag taagccaggg gagaccggcc ccaccaccat gtccagcggc
     3061 tgtaatcgag cgtggggaag ggattgactt caacactggt gctgaccgcc ttgccgagca
     3121 tgaatctgtc aacaaaggct gcaatggaag gggcctccgc tggcagcgtg ccgcagtgca
     3181 cgtgaccgcc gtcaatgtag aagccaaacc ggtcaccgat gcccagcatg ttgtagatcc
     3241 gctgcgtcgc ccgcgcagag atgtagttgg aacggttgga gagccagtaa taatcagtat
     3301 ttccggtttc aagcaatgcc cggggtgcaa tcatcgccat caattcgtgg tggtccgcag
     3361 gaagtttgta gacgttgtca cccgcaaact gcttcattgt ccccgcaaac cagtcataat
     3421 ccgtatcgtc tacgtcttcc accgaaccct gcggctcaat ctcacgcgag acgcgccatg
     3481 ccggtgcacc gccgccaccg ttttcctggg caatggtcag cgcgatacgc tcgtcgaacg
     3541 ctccggcgaa gagcgccatc tttccggcat acgagcaccc cgtcacggcc aggtgagaca
     3601 tatcaacggg cagtgggttg acggcctgat gggacgcgat ctccatgcca tctataaggc
     3661 ggctcacgcc ccaagcccag gccgcatatt cacccgaatt gctggtcccg gtgcatgttc
     3721 cggcgcagag ttcaggataa agctgataaa aggggtcctt cgtgtggtcg ctgggcgagg
     3781 aaaaggagta ggccgccacc tggttgtgca cgaaatccac ggtggccacc ggacggctgg
     3841 aaaaaacact cgcgggtaga aatccgtaat tcggaccgct ggcgctggcg aaaaacgtca
     3901 tggcaatcag cgcaggaaag ggtccgcttc ccattccgga tggaatgtag acgcctgaag
     3961 tgagcgtaag cgacttgcct ttccgggtga caatcactgt gagcgtgcct ttggccgatg
     4021 atgtggcagg aggaacatag ctggccgtaa tggtgcagtc tgagcagtcc ggcttgggtc
     4081 cgatttcgta cttttctatg gcggccttga tttcattgcg atggcgctcc cagctctgaa
     4141 aactggtgtc gcgccatcca tccataaaac ggaaagggtc cggcagaggt cgaaggatgg
     4201 ggagatggtc aaaatccggg aagaccggag catgataatg cgcgccggta ttctcaacgt
     4261 tgtagaccag cgggatgcca ggatcctcaa aggctttttt ctgggcggat gcagttgcaa
     4321 caaacaacag caacagcagt gaagacatcg cggacgtgat tggcttcata gtgtgcctcc
     4381 gggggcagga gttgcagacc acctcagaac agagggccag caggactttg ctggtggggc
     4441 ctgaggcaaa tttaattcgt gtcgcaaact atattgaaga gcaatcgctt gtcaagagaa
     4501 attttgattg aggatgaatg agcggaaacc aggcttttca taaaatttga aacacaatca
     4561 aaatgccgca cttgcgggaa agtggtgtgg cggaaaggcg atttttaaaa ggttttttgt
     4621 ggccaaacct cacatcgctg agattcttcc ttccttcggt cgtaagaatg agtggctcta
     4681 catatttact aacttgtctt tacatccccg taaaacttcc actgcgtatg gaagtctttc
     4741 gccagtttct ggttcgttaa gctggcgcga atcatctcaa ccggaatgct gttttctttg
     4801 agcactgcat cgtggaactg acagtttgtc atcttgccgg atccgaccag ctctttatgc
     4861 agtgcgtaaa tctgcattcc gcccaacagg tatgcggcct gatagagcgg cccatatccc
     4921 ccgccaagcg agcggcgcac ttctccgatt gcggcctgct tttcaaagcc aacgcggtct
     4981 acgagcagat caatgcactg ctgcggtgtc catttgccca gatgaaagtt gagtgagaag
     5041 atgatgcgtc cgcagcggtg gcgccgccat gccagcgcgc ctacgcgctc ttccggagtg
     5101 cggtcaaagc ccaggtccca catcagcatc tcccagtaaa gcgcccagcc ttcgatcagg
     5161 aatggagtgc ctccgaatgc ctggcggtag gcgcggaagc gctcttccat aaagccctgc
     5221 aattcgtggc cgggcagcat ttcatggaag accgtcgcgt gtgacatggc gatgttgttc
     5281 gcgcgcatcg tggtgatgcg ttgctcgtaa gacatcgtgt tggtcggata cgagacttgc
     5341 agcacttctc caccgagaaa gaacggcgag atgagctgct gctggggagg aatcattctc
     5401 atgcgccatg tctcgcgcgc cagcggcggt actgtaatca gatcatgctt gtcgaggaaa
     5461 tccagcgcct ggaagatata tttacgcgcg agttctgtct gctttcctgg ctcgacgtag
     5521 gtgttcttta ctttttcgag cgcctttttc cagtcgtcgc caaagcccat ttcgcgcgag
     5581 gccttcatca tctcgttgtc gttccaggcg aactcttttt ctgcgatggc gatgagttct
     5641 tccggggtgt agggaatcat ggcgaagttc agttcgcgta tcagcgcatc gcggccaatg
     5701 gcatggccga tgatgtcgct gctgtctccc ggatgcgccg aggccatgga ccgctgatag
     5761 gcttcctcat caccttcgcc gctgggcgga aggttttttg cttcgggagc aggatgcaga
     5821 ccgactactt tttctgagag aaatttgcca taagcatcga gcgcggcatc ggccgtcttg
     5881 tatggttcct cggcccacca ggtaaacatc ggatcgtagc cgtcgtaaaa ctcaaaccag
     5941 ttgtggagca cgtggcgcag cgtctcaagg ttctgcacgg cgcggtttgc ggcctcgcgg
     6001 cggcgctggt aagcaaggct ttttgtatct tctttgtttg cgcttagact cgcttccagt
     6061 tctttctgct tgtctttgca ggcctgctca atttgggcca gggtggccgc tgattg
//
LOCUS       DASRBV010000075         7583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_510571, whole genome shotgun sequence.
ACCESSION   DASRBV010000075 DASRBV010000000
VERSION     DASRBV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510571"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /gene="prfB"
                     /locus_tag="VFA02_03110"
     CDS             <1..151
                     /gene="prfB"
                     /locus_tag="VFA02_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265905.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZU08865.1"
                     /translation="MAKDHRTKVEVGDVDRVLDGYLEPFIRGYLLMKRNGGVPVAADA
                     GDDLE"
     gene            complement(430..1674)
                     /gene="fabF"
                     /locus_tag="VFA02_03115"
     CDS             complement(430..1674)
                     /gene="fabF"
                     /locus_tag="VFA02_03115"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZU08866.1"
                     /translation="MQRRVVVTGIGLLCGVGNTTPEVWNNLLAGKSGMAPITGFDTTG
                     FSVTFAAEVKNFDPLNFVDKKEARKMARFIHFALAATHEAMEMSGLKVTDAISDRVGV
                     HIGSGIGGFDIIEREHSAMLQGGPRKISPFFIPAAIINLAAGQVSIKYGARGPNEATA
                     TACTTSAHSIGDAYRIIERGDADVMIAGGAEAAITPMGVGGFAAMRALSTRNDEPERA
                     SRPFDKDRDGFVVGEGAGILILEDLEFAKARGAKILAEIIGYGMSADAHHITSMAPEG
                     EGCYRAMKHALDSAHVQPHEVDYINAHATSTPVGDVLESAALQNLFGEHAASGKLLVS
                     STKSMTGHLLGGAGGLEAGITIKALLEQVAPPTINLENPDPECRLNYVPNKPQQAKIH
                     IALSNSFGFGGTNGSLIFRKWE"
     gene            complement(1677..1922)
                     /gene="acpP"
                     /locus_tag="VFA02_03120"
     CDS             complement(1677..1922)
                     /gene="acpP"
                     /locus_tag="VFA02_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU08867.1"
                     /translation="MAAVEDKVKQIIVEQLQVDEAEVTPSASFQEDLGADSLDVVELV
                     MQFEEAFDLEIPDEDAEKIKTVKDAIDYIEKNAKGGK"
     gene            2156..3523
                     /gene="rmuC"
                     /locus_tag="VFA02_03125"
     CDS             2156..3523
                     /gene="rmuC"
                     /locus_tag="VFA02_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZU08868.1"
                     /translation="MPLVTLFVVLALLAGVAIGYWMRHLGARSELVEKSAALQSVTAQ
                     LAEREKSLREVIEKAESLGLEKARLEERLAAEQEALKQAKAALEESFRSAASRVLNEN
                     SQAFLQLARQELGKQQQVAEHVLSGKEQAIAELLKPVRETLEKLEANTQQLEVRREGA
                     YQSLKQLVEGMQVSQADLRRETSQLISALRAPKVRGNWGELQLKRCIEFAGMVEHASF
                     DTEVFVRGEDNAIRPDCVIHLPNERTIVIDVKTPFDAFLEAMSAQDEQTRSVKLAAHA
                     ARVKEHLSQLSAKSYWKQFKDSPDFVVCFLSSEVFFSAALEQDPGLIEFGSASNVILA
                     TPTTLIALLKAVAYGWQQLEITRNAQIIRDTGEKLYEKLATAHRYFSQMGNALTNAVK
                     HYNNLVGCLEGRGSVFTHARTLHELAIGQAELPEMPRLESVTRDLKASDWKPEEVEKL
                     FSETD"
     gene            complement(3555..3845)
                     /locus_tag="VFA02_03130"
     CDS             complement(3555..3845)
                     /locus_tag="VFA02_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08869.1"
                     /translation="MKDRRKTEPHDLEEHASTKHRPPTGVQSAPAPVEPEDAYLTGAS
                     RSLQRRLLGLNIENGQAQRHPDTPAGIHSTGSFTGKRRNPEPYPPLKTQNEG"
     gene            complement(3952..4929)
                     /locus_tag="VFA02_03135"
     CDS             complement(3952..4929)
                     /locus_tag="VFA02_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZU08870.1"
                     /translation="MSTFSIGVDLGGTNLRIAAFTQEWERLKAVSMPTRVSAGPQAVL
                     DDMSSAIRQIVSEFGTEREFAGVGIGCPGPLELPSGRVLTPPNLPGFEGLELKTELER
                     ILECPVVVECDANAAALAECHAGAGKATGLQSLCMLTLGTGVGSGIILNGHVWHGFAG
                     MAGEAGHVPVYHDGLLCGCGSRGCLEQYASATAIARAGRKLVAAGSAGNGHDTVTARS
                     IAEAALAGDAESQEIYDEVGRALGIGLAGLVSTLNLPLYVIGGGVAAAWALFAPAMFA
                     ALEADSYIYRLTKPADPNVPEKSKTNVVPASLGPDSGLLGAAMLPFFER"
     gene            5098..5751
                     /locus_tag="VFA02_03140"
     CDS             5098..5751
                     /locus_tag="VFA02_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU08871.1"
                     /translation="MDQFPLPDWCRALLDPQDEHGKRRQLLLQAAFDVIAEAGFEGLR
                     TRSVAARAGVNIATLHYYFPTKQDLIEGLAQFIGAKFVSIHGPRPRPSGHPALDRLRQ
                     EFSDARYYVAHEPKMLLVMQEFAMRGKRDPMVQKVVDQMHFYWRQGLEQMVLDGVASG
                     IFRTDIPPQELLAILMSIFSGIAITGGDRIGEIERVTETWILSSKSRKHSRRTGAKK"
     gene            5748..7022
                     /locus_tag="VFA02_03145"
     CDS             5748..7022
                     /locus_tag="VFA02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU08872.1"
                     /translation="MSGNTFDVAIIGGGIGGLCLAQGLKKAGIHVTVYERDETPTSRL
                     QGFRIHIDPQGSTALHECLPEHLWKIFDATGGDYAQGMTIVTEQLEELLRFRGDDVQN
                     DPVARHRSISRITLRHVLLAGLDNSVRFQKRFEHYEEMPNGRFALHFEDGSRAEADVV
                     IAADGVNSRIRKQYLPHAEPVDTGVVGLGGKVPLTDGVMALAPHYLLNGPVMVMPPGP
                     CSLFMAIWKRSPQASGPLRMLGIHEVPQGDEDYLILGFGGRPEYFGLTGNPDSISNQA
                     WKEAMRRIVMNWHPDLRKLVEMLDAGSISVNRLRTSRPVAPWETTRITLLGDAIHSMT
                     PYRGIGANIALKDAALLCSKLAEAHRGEKSLLEAIDEYETVMREYAFAAVEASRKSME
                     QSVGERKMFAVAKTAMRVINAVPPLRRKLLSA"
     gene            7183..>7583
                     /locus_tag="VFA02_03150"
     CDS             7183..>7583
                     /locus_tag="VFA02_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08873.1"
                     /translation="MAKIGSYQDGPAIAEHEQPDEFLRPAVKVRTRLQRPIPRMEEDG
                     PAEDEVFLRARKRVPVRKGILPPFLTKTIPGRVILGFSAVLVVILGAMIVMAVRNFLD
                     HDPHFRIESATNIQIDGNSQLTRPELLSVFGA"
BASE COUNT         1623 a         2162 c         2140 g         1658 t
ORIGIN      
        1 catggccaaa gaccatcgaa cgaaggtcga agtcggcgat gtagaccgcg tgctcgatgg
       61 ctacttagag ccatttattc gcggatatct tctgatgaag cgcaatgggg gagtgcctgt
      121 ggcagcggat gccggggatg atctggagta aaaccagggc gtggggctta agcatgtcca
      181 tcaggtgcag caagctttaa cactcgttga tttcaaaaac tcctcaatct cactgaactt
      241 gaaaatatgc cccttttgct tcgctgaatt cccagccgct tccacttgcg tcaaatcccc
      301 ggcttcgtat tgatatttat tcccggtctt aggggcaatg acgaagatgc tgcggacgcc
      361 catttctcgt aatcagccag ctataagtgg aaaatgctat gggccgcgcg cggagtgttc
      421 tccgcgcggt cattcccact ttcggaaaat aagcgagcca ttggtgccgc cgaaaccgaa
      481 cgaattggac aacgcaatat ggatctttgc ctgctgcggc ttgttgggaa cgtagttcag
      541 ccggcattcc ggatcggggt tttcgaggtt gatggttggc ggagcaacct gttccagcag
      601 ggccttgatg gtaatgcctg cttccagtcc gccggctccg ccgagcaggt gcccggtcat
      661 ggatttggtg gagctgacga gcagcttgcc gctggcggcg tgctcgccga agagattttg
      721 cagtgccgcg ctttcgagta cgtctcccac cggagtggaa gtggcgtggg cgttgatgta
      781 atccacctca tgcggttgga catgggcgct gtccaacgcg tgcttcatgg cacggtagca
      841 gccctcgcct tccggggcca tgctggtgat gtggtgggca tcggcgctca tgccgtagcc
      901 gataatttcg gcaagtatct tcgctccgcg cgctttggcg aactccaggt cctccagaat
      961 gaggatgcca gcgccttcgc cgacgacaaa accatcgcgg tctttgtcaa agggacggct
     1021 ggcacgctcc ggctcatcat tgcgcgtgga aagggcgcgc atcgcggcga atccgccgac
     1081 gcccatggga gtaatggcgg cttctgctcc gccggcgatc atcacgtcag catcgccgcg
     1141 ctcaataatg cgataggcat caccgatgga gtgcgcgctg gtggtgcagg cggtcgcggt
     1201 ggcctcattg gggcctctgg cgccgtattt aatgctgacc tgtccggcgg cgaggttgat
     1261 gatcgccgcc ggaataaaga acggcgatat cttgcgcgga ccgccctgca gcatggcgct
     1321 gtgctcgcgc tcgataatgt caaacccgcc gatgccggaa ccaatgtgga caccgacacg
     1381 gtcggaaatc gcatcggtga ccttcaggcc ggacatctcc atggcctcat gcgtggcagc
     1441 aagagcaaag tggatgaagc gcgccatttt ccgcgcttcc ttcttgtcca caaaattgag
     1501 cggatcgaag ttcttcacct cggcagcgaa ggtgacagaa aatcctgtcg tatcgaagcc
     1561 ggtgatgggg gccatcccgc tcttgcctgc aagcagattg ttccagacct caggcgttgt
     1621 gttgcctacg ccgcacaaca acccaatgcc cgtgactacg acgcgccgtt gcaaggttac
     1681 ttgccacctt tcgcgttttt ctcgatgtaa tcaatcgcgt ccttcacggt cttgatcttc
     1741 tcggcgtctt cgtcgggaat ctcaaggtcg aaggcctctt caaactgcat cacaagctcg
     1801 accacgtcga gcgagtctgc gcccaggtct tcctgaaagg acgcgctggg ggtcacttcg
     1861 gcttcatcca cctggagctg ctcaacaata atctgtttta ccttgtcttc tactgctgcc
     1921 atgcgtttct cctgtcttat gttacggcaa agaaatgaaa gaactttccg ggaacggcac
     1981 acacgaatac cgcactcaga aatttcaagt ctactgagga ctgggcatcc aggcaaggac
     2041 tggccgcgcc cgggcaatcg cgtttcaggg gactggcagg tggtcgcgct tccctcaata
     2101 tgaaatgctg ctgccctctt tcatctccca cgctttcagt gcgactatat tcgctatgcc
     2161 cctcgtgaca ttgtttgtag tcctcgccct cctggcggga gtggcgattg gttattggat
     2221 gcgccatctg ggggcgcgct ctgagctggt tgaaaaatct gctgctctgc aatcggtcac
     2281 ggcccagctt gcagaacggg aaaagtccct gcgcgaggta atcgaaaaag ctgaatctct
     2341 tggactggaa aaagcacgtc tggaagaacg cctggccgcc gaacaggagg ccctgaagca
     2401 ggcaaaagcg gcgctggagg aaagcttccg cagcgcggca tcgcgcgtgc tgaatgaaaa
     2461 cagccaggcc tttctgcaac tggcccggca ggaactaggc aagcagcagc aggttgccga
     2521 gcacgtcctt tccgggaaag agcaggccat cgcagaactg ctgaagcctg ttcgcgagac
     2581 cctggaaaaa ctggaagcca atacacagca actcgaagtc aggcgcgagg gcgcatacca
     2641 atcgctgaag caactggtcg aaggaatgca ggtatctcag gccgacctca gacgcgagac
     2701 ctcgcagctt atctcggccc tgcgtgcgcc aaaagtacgc ggcaactggg gagaattgca
     2761 actgaagcgt tgcattgagt tcgctggaat ggtggagcac gcctcgtttg acaccgaagt
     2821 gtttgtgcgc ggagaagaca acgccatccg gcctgattgc gtgattcatt tgcccaatga
     2881 gcgcacgatt gtgattgacg taaagacgcc gtttgacgct tttctcgagg cgatgagcgc
     2941 gcaggacgag cagacccgct cggtgaagct ggccgcccat gctgcgcggg tgaaagaaca
     3001 tctcagccag ctttctgcca agtcctactg gaaacagttc aaagacagtc cggattttgt
     3061 cgtgtgtttt ctttcaagcg aagtgttttt cagcgccgcg cttgaacagg acccaggcct
     3121 gattgagttc ggcagcgcgt cgaatgtgat tctggctact ccgaccacgc tcatcgccct
     3181 actgaaggcc gtcgcttacg gctggcaaca gcttgaaatt acgaggaatg cgcaaatcat
     3241 ccgtgatacg ggagaaaagc tatatgaaaa gctggcgacg gcgcaccgtt acttctccca
     3301 gatgggaaac gccctcacga atgccgttaa gcactacaac aacctggtag ggtgtctgga
     3361 agggcggggc agcgtcttta cccatgcccg gacattgcat gaactggcca tcggacaggc
     3421 cgaactgccc gaaatgccca ggcttgaatc cgtaacgcgc gacctgaagg ccagcgactg
     3481 gaaaccagaa gaagtggaaa agcttttttc tgaaacagac taaggatgaa tgcgaagaac
     3541 accctcggcg cacttcagcc ttcattttgt gtctttagtg ggggataggg ctcaggattt
     3601 cttcgctttc ccgtgaacga ccctgtcgag tggattcccg caggtgtgtc cggatgccgc
     3661 tgcgcctgtc cattctcaat gttcaaaccc agcaggcggc gttgcaggga gcggctggcg
     3721 cctgtcagat aagcatcttc cggctcaacc ggggcaggag cggactggac gcctgttggc
     3781 ggtctgtgct tcgttgaggc gtgttcttca agatcatgcg gctctgtttt tctgcgatct
     3841 ttcatgtggc accttctcaa ctctcagaag agttagatgc gcataaaaca tcgcctgcgc
     3901 cgctgaatat aaaaatccag ctctgcgttt cgcggtaatg tctttcattc ctcagcgctc
     3961 aaagaacggc agcatggcgg ccccaagcaa ccctgaatcc ggacccagac tcgcaggcac
     4021 cacattcgtc ttcgattttt ccggaacatt ggggtccgcg ggcttcgtca ggcggtagat
     4081 atagctgtcc gcctccaaag ccgcgaacat cgccggagcg aacagcgccc atgctgctgc
     4141 aacgccgccg ccaatcacat acagaggcag gttcagcgtg cttaccaggc ccgccagtcc
     4201 gattccgagc gccctgccta cttcgtcata aatttcctgc gattcagcat cgcccgccag
     4261 cgcagcctct gcgatcgagc gcgcagtcac ggtatcatga ccattgccgg cgctgcccgc
     4321 ggccacaagc tttctgcctg cacgcgcaat cgcagtcgct gaagcatact gttccagaca
     4381 gccccggctg ccgcatccgc aaagcaggcc atcgtgatac accggcacgt gcccggcctc
     4441 gccagccatt ccggcaaagc catgccagac atgcccgttc aggataatgc cactgccgac
     4501 gccggtccca agcgtcagca tacagagtga ttggagacct gtcgctttcc ccgcgccggc
     4561 atggcattcg gccagagcag ccgcattcgc gtcgcactcc acgactaccg ggcattccag
     4621 tatccgctcc agctccgttt tcaattcaag cccttcaaat ccgggaagat tgggcggagt
     4681 cagcacgcgt ccggaaggca gctccagcgg tcccgggcat ccgattccaa ctccggcaaa
     4741 ctcgcgctct gttccaaatt cagaaacaat ctgccggatg gccgaggaca tatcatcgag
     4801 cactgcctgc ggcccggcac tcacgcgtgt cggcatggaa accgccttta gccgctccca
     4861 ctcctgagta aatgcagcaa tgcgcaggtt tgtgccgcca agatcaacgc caatcgagaa
     4921 ggtactcatg tcagcttcag tgtatatgat aacgttatct atcattgttc ccggaaattc
     4981 ctgcaatgcc cctctgaaac cgatacgttt acccgacgtc gcaagtttct cgtgtgtcta
     5041 ctatataatc aatcagttga ttgacattca atcaactgat tgattatatt tctcctcatg
     5101 gaccagtttc cgcttccgga ttggtgccgc gccctgctcg acccgcagga cgagcatgga
     5161 aagaggcggc agctgttact ccaggcggcc ttcgacgtca ttgctgaagc tggttttgag
     5221 ggcctgcgta cccgctcggt tgcggcgcgg gcaggggtca acattgcaac cctgcattat
     5281 tacttcccca caaagcagga tctcatcgag ggtctggcgc agtttatcgg agcaaaattc
     5341 gtgtctattc atgggcctcg gcccaggcca tcagggcatc ccgcgctgga ccgcctgcgg
     5401 caggagtttt ctgacgcgcg atattacgtc gcccacgagc caaagatgtt gctggtgatg
     5461 caggaattcg ccatgcgtgg caagcgcgat ccgatggtgc aaaaggttgt agaccagatg
     5521 cacttctatt ggcggcaggg actggagcaa atggtgctcg atggagtggc cagcggcatc
     5581 ttccgtactg acatcccgcc acaggagttg ctggccattt taatgtccat tttctcaggc
     5641 attgccatca caggcggcga caggattggt gaaattgagc gggtcacaga aacatggatt
     5701 ctttccagca agagcaggaa acattccagg cggacaggag caaagaaatg agtggcaata
     5761 catttgacgt agcgattatc ggcgggggca ttggcggcct ttgtcttgca cagggattga
     5821 agaaggccgg catccacgtc actgtctatg agcgcgatga gacacccaca tcgcgcctgc
     5881 aggggttccg catccatatt gatccacagg gtagtactgc cttacatgaa tgcctgccgg
     5941 aacatctgtg gaagatcttc gatgccaccg gaggcgacta tgcgcaaggc atgaccattg
     6001 tgactgagca gcttgaggaa ctcctccggt ttcgcggaga cgatgtccag aatgatccgg
     6061 ttgcgcgcca ccgctccatc agccgcatca cattgcggca cgtactgctc gccggactcg
     6121 acaatagtgt gcgtttccag aagagatttg agcattacga agaaatgccg aatggccgct
     6181 tcgcgctgca tttcgaggac ggcagccgcg ctgaagccga tgttgtcatt gccgccgatg
     6241 gagttaattc gcgcattcgc aagcaatatc tgccccatgc cgagccagta gatacaggtg
     6301 tcgtcggtct tggcggtaaa gtccctctca ccgatggggt gatggccctt gccccgcatt
     6361 atctgctgaa tggtccggtt atggtaatgc cgcccgggcc ttgcagcctg tttatggcga
     6421 tctggaagcg ttctccacaa gccagcggcc ctctgcgtat gcttggaatt catgaggtcc
     6481 ctcagggcga tgaggactac ctgattctcg gttttggggg aaggccggaa tatttcggtc
     6541 tgactggcaa tcctgactcc atcagcaatc aggcatggaa agaagcgatg cgccgcattg
     6601 ttatgaactg gcatcccgac ctgcgcaagc tggtggaaat gttggatgca gggagtattt
     6661 ccgtcaatcg cctgcgcaca tcgcgtccgg tagcgccctg ggaaacgacc cgtatcaccc
     6721 tgctaggaga cgctattcac agcatgacgc cgtatcgcgg catcggagcg aacattgcgc
     6781 tgaaagatgc agcactgctg tgttcaaaac tcgccgaggc ccatcgcgga gaaaaatccc
     6841 tgctcgaagc cattgacgaa tacgaaaccg taatgcgcga atacgcattt gctgcggtcg
     6901 aggcatcccg gaaatccatg gagcaatctg tcggagagag aaagatgttt gctgtagcga
     6961 aaacggccat gcgagtcatc aacgcggtgc cgcccctgcg gagaaagcta ctctcggctt
     7021 agaccgaacc tggcaggagg cttttgaggt tccggatgcc gcaggtaaac tcggttttct
     7081 ctcaagattg ccttcttaga ttcaccctca gggaattttg ctctttccac agcacgcggc
     7141 acgctatagt ggtcatggcg actctcacga ctcggctcgg gcgtggcgaa aatcggttcc
     7201 tatcaagacg gccccgcaat tgcggaacat gaacagccgg acgaatttct gcgtcctgcg
     7261 gtcaaggtcc gcacgcggct ccagcggccc attcctcgta tggaagagga cggacctgct
     7321 gaggacgagg tgttcctgcg cgcgcgcaag cgcgtcccgg tgcgaaaggg aattttgcct
     7381 ccgtttctca ccaaaaccat tcctgggcgc gtcattctcg gtttttccgc cgttctcgtt
     7441 gtaatcctgg gcgccatgat cgtgatggcg gtcaggaatt ttctcgatca cgatcctcac
     7501 ttccgcatcg agtcggccac gaacattcag attgacggta acagccagct cacgcgaccg
     7561 gaattgcttt ctgttttcgg cgc
//
LOCUS       DASRBV010000076        10674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_612423, whole genome shotgun sequence.
ACCESSION   DASRBV010000076 DASRBV010000000
VERSION     DASRBV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612423"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFA02_03155"
     CDS             <1..137
                     /locus_tag="VFA02_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HZU08874.1"
                     /translation="ILKRVFGFEKVRYRGLSKNHNRLCACFALVNLYLHRKRLALAGA
                     "
     gene            448..1653
                     /locus_tag="VFA02_03160"
     CDS             448..1653
                     /locus_tag="VFA02_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08875.1"
                     /translation="MLLTGTANAQRYSNNEVTVGLQAQTWGTKYINSSNEFAYHHYLQ
                     YPTPYFTYTRNLSSTLAVEGTVEPWTQFFRTNALESGHGTLALGGVKAGWRGREWGFY
                     GKTQVGIASWSCGGWDYAPHPYSKCSRITNFALEYGGVVERHLFGRYALRMDAGHLLS
                     TEFDQVLKHFPNGGSVYRGGGTLQHLDVRIGITRSFGVVRDAGSERVPEKASWDLGAS
                     FALQPRDEALYSILDVYPGPGLWASWNFCRHVSWDSAIIHSGPYRKEQYVFSVPQAGG
                     RALEVLTGLKMGLRRDHMGYFAKLRGGTITFGEAERRIGLLPDGTFFIRRGMFTNPVL
                     DVGGVWEVYPSRHTVLRFDAGSATIFYQPKDLWQYVPVHGVEVGTKYSVPGQAQTSLL
                     LSFGVGIRF"
     gene            complement(1625..2062)
                     /locus_tag="VFA02_03165"
     CDS             complement(1625..2062)
                     /locus_tag="VFA02_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU08876.1"
                     /translation="MKATKNLWEQAKDCKRQVSLSFLPMERILRQPVFLFGNSLGGYL
                     SVALAFRRSEIAAISVVSCGRPEGHLTDRPRKLRVLISEGADDTIVPPSEAEALAQYC
                     RTYGLAIEIDMYAGENHYLSQGMKAEVLNKTVRLFKTVSLPRN"
     gene            complement(2019..2270)
                     /locus_tag="VFA02_03170"
     CDS             complement(2019..2270)
                     /locus_tag="VFA02_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08877.1"
                     /translation="MNCAEFQEKLPELFESGVDVSKDPHVKSCENCAALVRDLEYIAS
                     QAKLLLPIHDPSPKVWENIQSAIRGDEGNKEPVGASKRL"
     gene            complement(2303..2932)
                     /locus_tag="VFA02_03175"
     CDS             complement(2303..2932)
                     /locus_tag="VFA02_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08878.1"
                     /translation="MNADPHPAKPVQHMRRNPPIEGEAEAISRAQAGDARAFETLYAL
                     HKRRVYSLCLRMLGNVAEAEDLTQEAFLQLYRKIGTFRGDSAFSTWLHRLAVNVVLMH
                     LRKKGLPQVSLEETLEPSQDDGPRKDIGERDLMLSGSLDRVALERAVENLPPGYRLVF
                     VLHDVEGYEHNEIAEMLDCSIGNSKSQLHKARMKLRDLLRSGERKESLV"
     gene            complement(3093..4118)
                     /locus_tag="VFA02_03180"
     CDS             complement(3093..4118)
                     /locus_tag="VFA02_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZU08879.1"
                     /translation="MLLTQFTERYSRQVLYPGIGEEGQQKLARAHVAIVGCGATGAAA
                     ASLLARAGVGTLTLIDRDFVEESNLQRQVLFDEADAAQSLPKAEAARRKIASFNSAIT
                     VHAHVADLVPANIHDLLYDAAIVLDATDNFETRYLLNDYAVEQNRPWIYAAAIGAYAV
                     TMNVLPGETACLACIFPRPPGGTVETCDTAGILNTAVNLAASVEATEAIKLIVGARDK
                     VRRSLLSFDLWTNERSEVPTVRPRHDCEVCQHHNFAHLRGEGRPHITLCGRNSVQIHE
                     RHRPVDFSEMEKRLRPHGTVRYNNMLLRFEHGDYVLTLFADGRAIIQGTTDVTQARSL
                     YARFIGS"
     gene            4225..7356
                     /locus_tag="VFA02_03185"
     CDS             4225..7356
                     /locus_tag="VFA02_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZU08880.1"
                     /translation="MDFRCKSWWRICWICLLVFAWCEVALAKAQANESAGQTGDVTLR
                     TWSGLQIDGIQFKGIEAERLDPLPQKLAQQPGQPLDPDKVRQSLRRLYETGLYKTIQV
                     EGVRHGNTVTIIFDGTPNLFIGRITVEGVRAERLSGVLERSTRMNPGTVFTDDKLQQA
                     ESQLRQTLEENGYYEAKIFASTSIDQPNSQVNVAFQIELGKQARVGNVAVTGDSGMST
                     EKFRKTGKLKEGSKVTRNTVSNALTRLRKNYQKNDRLEAAIKLDSREYRPPVNHLDYG
                     FTANRGPLVKIQVEGTSLSKGKIKNLVPVYEEGTVDEDLLNEADRRIRDYFQRQSYFD
                     AKVTHSEQQVDPRHAVITFHVDLGSKHRVEAVHIKGNKYFDSDVIRQRLSVTPASVVN
                     RHGMYSYALAQADASSIEGLYQSNGFTDVKVTPEIQDVDTGPNGQRLKTDLLTVTYRI
                     EEGPQTRLGEYEIHGAQKIPLAQLTPLLNTQSGQPYSAANINNDRDTLLSYYLSHGFD
                     KAQVQVTQRRSSARPNTMDVAMDITEGEQVFVNRVLISGLHYTRPSTVEDRVLLHPGD
                     PLDQTALLDTQRKLYDLTLFNQVNTAVQNPTGDELRKNVLVQFSEARRWDLNYGFGFQ
                     AQTGNPQTECKDLTAEELIKLGINPSTFHCSPNGKTGVSPAFLFNVSRINLRGTDKSI
                     SLNTTYGTLEQRVQMIFSNPHLFDNPKFDAILSGGYTNAQDVTTYAASRLEASMRVTN
                     HVNKPNTLIYLWSYRRVRVDPNSVQVAPNEIPLVSQPVRVGGPGITWIRDTRVPTPLD
                     ATKGTYNTVQDTWADSHYGSQANFNRIDATNSSYYVFGKQNKWVVARSTRLGVERSYG
                     ADKYELIPLPERLYAGGAQSHRGFGINSAGPRDSLTGFPIGGAGAFINSTELRMPNPT
                     LPYVGNSLGFVLFHDMGNVFENSSDIWPSMFRIKQPHSYTCKDTNEADQSKPHEHSST
                     GGAGTCSFNYFSHAVGLGLRYHTPVGPLRLDFSYNLNPPIYPILIDYSGGPVPRIGQA
                     PHFNFFFSIGQAF"
     gene            7356..8114
                     /locus_tag="VFA02_03190"
     CDS             7356..8114
                     /locus_tag="VFA02_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU08881.1"
                     /translation="MAWRTQFGQRTACAILCVCLCTPVWPQTSSASASPVVLDRVIAI
                     INGDVLLESDVREEMRLSVLQPISVPANQNTEIRAAQRLITRTLILRQMKEQEAINYS
                     VSDEDVRKSLEELRRQIPGCGPMHCATEAGWQAFLQKNGLTEQEVDARWKERLEILKF
                     IDQRFGVSIHISRQDIENYYRKNFLPAFAKTKQKPPTLESVAPRIREILLQQQVNSQL
                     RDWLQSLREQGSVQILDPKYGQSTGNDDDTGGGA"
     gene            8111..>10674
                     /locus_tag="VFA02_03195"
     CDS             8111..>10674
                     /locus_tag="VFA02_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08882.1"
                     /translation="MSTQPALPATARRLWLRAGLVVVVVLLAVIGGLIWYASTPQFEN
                     LVREKLVAVLEQSTGGRVELQAFRWSLRHLAFEADGLTIHGLEAANEAPYVHVERIYV
                     RVRILSLLGPKKIDLNYLEADKPAIHLIIYPDGSTNQPKPKTQHQGKSVKDEIFDLQA
                     GRTEIRDGVALINERAIPFNFAANDLGVVVTYTPANDHYLATLQATDMVAQRGVRPPL
                     RSRLDLAADIGRNTLDLTKFELRTGPSSLEATAAVKDFANPHWTLIARGKVDVREVMV
                     LEPVEGVDSGVATLDLKGQGTKTQFLMDGLVRVAGAAYHTGAVHITGANADMALHMTH
                     DDLALTGIRVKLNRGGAVDAEMRIARWLEPARKGSIRARIGGFSLRSVMAMVAPPHYE
                     DLGFDTWVAGDTFVDWTGNASDLSGRANVTLTPSRQTPNGEVPLSGIVDATHFQKNGT
                     VVVKQLQAQTPATRFDVSGGLGVYPLSRPSALQVNVQTTNLAEFDKALSTLGVASHGK
                     SGVQALPVRLQGQAEFHGTVSGTLSSPDAKGHLTAGNFDLLLAGRTIHWDSLVTDAEY
                     SPQRIAVQQATLTHGTTTVHASGQLQAHRLSERHYAFDDESAIQADLRVQNAELADVI
                     AISGRNLPDTSGTVTLNAHAGGSLSNLNGGGHISIAGGKIYGEPYRSLNADLRFTGDE
                     VGATKLTFLENGGQLTAEGGYHVRDKQFHFHAQGKGFDLSHIERLQNKRIPVTGQLVF
                     TASGSGTLESPNLQVNAHLTHITVGNEINGAVDLDAHTGHGALLYTTTGHLGQALLQI
                     NGQTAITGDFQTQAQAQLTNLDIDPFLKMFEVEGVQGHSSIAGLVSVSGPLRAPRQ"
BASE COUNT         2447 a         2734 c         3115 g         2378 t
ORIGIN      
        1 ggattctgaa gcgggtgttc gggttcgaga aggtgagata ccggggactg agcaagaacc
       61 acaaccggct gtgcgcctgc tttgcgctgg tcaatctcta tctgcaccgc aaacggctgg
      121 ccctggccgg ggcgtagtgt cgctggaagc cgcataaaca acgggttcta agccgataga
      181 acgagcacaa cagcaaacat aagcgtttct atcatcctca aaatccgctc gcgcttcccg
      241 cgttcctcaa atcagccggt tgctcagagg ttccctaatt aatttattaa gagtatgtaa
      301 tttggcgcgc ccagtcggtg cttgtgtaga actgggcgca gtcaaaccgg tttgtttctt
      361 ctcccaatct aactctaagg cttttttgag aaaccaatgc attcacaaat ttggtacttg
      421 cttttcgcac tcttcacttt tctccccatg cttttgaccg gaaccgccaa cgcgcagcgg
      481 tactcaaata atgaagtgac ggttggactg caagcacaaa cctggggaac gaaatacatt
      541 aatagcagca acgaatttgc ctaccatcat taccttcagt atccgactcc gtactttacc
      601 tacacgcgta acttgtcttc cacattggcc gttgagggca cagttgaacc ctggacgcaa
      661 ttctttcgca cgaatgctct ggaatcaggg catggaacgc ttgcgctcgg gggcgtgaag
      721 gctggctggc gcggaaggga gtggggattt tatggaaaaa cacaagtagg tatcgcaagc
      781 tggagttgtg gaggctggga ctatgctccg catccatata gtaagtgctc acggattacg
      841 aatttcgcac tagaatacgg gggcgtcgtt gaacgtcatc tttttggcag atatgccctg
      901 cgcatggatg cagggcactt gcttagcacg gaattcgatc aagtgctaaa gcacttcccg
      961 aatggtgggt cagtctaccg agggggtgga acactacaac acctggatgt tcgtatcgga
     1021 atcaccagaa gttttggagt cgtgcgtgat gctggtagtg agcgtgtgcc agagaaggca
     1081 tcctgggact tgggcgcgag ttttgctctt caaccccgag atgaagcatt gtattccatt
     1141 cttgatgtgt atcccggccc cggcctctgg gcttcctgga acttctgccg gcatgtctca
     1201 tgggacagcg ccatcattca ttccggtcct taccggaaag agcaatatgt attttctgtt
     1261 cctcaggcag gagggcgcgc gctggaggtc ctgaccgggc tcaagatggg actccgccgt
     1321 gaccacatgg ggtacttcgc caaattgcgc ggcggaacca ttactttcgg ggaggccgag
     1381 cgccggatcg ggctattgcc agacggaact tttttcatca gacgtggcat gttcactaat
     1441 cccgtcctgg atgtaggcgg ggtctgggaa gtctatccat cccgccacac ggtcctgcgt
     1501 tttgacgcag gaagcgccac gattttttat caaccgaagg acctatggca gtatgtgcca
     1561 gtgcatggtg ttgaggtcgg cacaaaatac agcgtgccgg gacaggcgca gacgagcctc
     1621 ctgctcagtt tcggggtagg gatacggttt tgaacaggcg gactgttttg ttcagaactt
     1681 ctgccttcat tccctgggaa agatagtggt tctcccccgc atacatatct atctcgatag
     1741 caagaccgta tgtgcggcaa tactgggcca gcgcctcagc ctccgatgga ggcacgattg
     1801 tgtcatcagc gccctctgaa ataagaacac gcagtttacg tggtctatcc gtcagatggc
     1861 cctccggcct accacaactg actacgctga tggctgcaat ttccgagcgc cggaaggcaa
     1921 gggcaacact cagatacccg ccgagcgaat ttccgaaaag aaacactggc tgacgtaaaa
     1981 tgcgttccat cggcaaaaat gacagagata cctgtcgctt acaatctttt gcttgctccc
     2041 acaggttctt tgttgccttc atcgccgcgg atcgctgact ggatgttctc ccagactttg
     2101 gggctggggt cgtggatggg cagaaggagc tttgcctgag aggcgatata ttcgaggtcg
     2161 cgcaccagtg cggcgcagtt ttcgcagctt tttacgtgcg ggtctttgct gacatccacg
     2221 cccgactcaa ataactccgg tagtttttcc tgaaattccg cgcagttcat ggtttcccta
     2281 tcttgctcgt tacggcaact gctcatacca gcgactcctt gcgttctccg gagcggagca
     2341 ggtcgcgcag tttcatgcgc gctttgtgca gttgggactt gctgttgccg atggaacagt
     2401 cgagcatttc ggcaatttca ttgtgttcat agccttccac atcatgcaga acgaagacca
     2461 gcctgtaccc tggaggcaga ttttcgacgg cacgttccag cgcaacccgg tccagagagc
     2521 cagagagcat caggtcacgc tcgccaatgt ctttgcgcgg gccgtcgtcc tgtgaaggct
     2581 ccagagtttc ttcaagcgat acctggggca ggcccttttt gcgcaggtgc atcagcacta
     2641 cattcacggc aagccggtgg agccacgtgg aaaaggccga atcgccgcgg aaggtgccga
     2701 ttttgcgata gagctgaagg aaggcttcct gtgtgaggtc ttctgcctcg gctacattac
     2761 ccagcatgcg caggcacagg gaatagacgc ggcgtttgtg cagagcatac agagtttcaa
     2821 acgccctggc atcgcctgcc tgggcgcggg agattgcctc tgcttctccc tcaatgggag
     2881 ggttgcgacg catatgttgt actggtttgg cgggatgagg gtctgcgttc attcgcactc
     2941 taccattctt gaaagttcca tactttgagg cttccgtcaa tccactgcct gttccagatg
     3001 gcatagcgag gttcatgacg cattgtccgt aaaaagatgg tctggaaggt aaattagacg
     3061 tacgcaaata gcgaacggtt gcctgccctg cattatgacc cgataaagcg cgcgtacaaa
     3121 ctgcgggcct gggtgacgtc ggttgtgccc tggatgatgg cgcgtccgtc tgcgaagagt
     3181 gtgagcacat aatcgccatg ttcaaagcgc aaaagcatgt tgttgtaacg gacggtgccg
     3241 tgagggcgaa ggcgcttttc catctctgag aaatcaacgg ggcgatggcg ctcgtgtatc
     3301 tgcacggagt tgcgtccgca taacgtgatg tgcgggcggc cttctccgcg aaggtgggca
     3361 aagttgtgat gctggcagac ttcgcaatca tgccgcgggc ggacggtggg gacttctgag
     3421 cgctcattcg tccagagatc gaaggagagc aggctgcggc ggactttgtc ccttgctcct
     3481 acgatcagct tgatggcctc ggttgcttct acggaagcag cgagattgac ggcagtgttc
     3541 agaataccgg cggtgtcaca ggtctcgact gtgccgcctg ggggcctggg aaagatgcag
     3601 gccaggcagg cagtctcgcc tggaagcaca ttcatggtga cggcgtaggc gccgatggcc
     3661 gcggcgtaaa tccagggcct gttctgctcg acggcgtaat cgttcagcaa atagcgggtc
     3721 tcgaaattgt cagttgcgtc gaggacgatg gcagcatcat aaagtaagtc atgaatgttc
     3781 gcggggacga ggtcagcgac atgcgcgtgg acggtaatcg cactattaaa gctggcgatt
     3841 ttgcgccgcg cagcttccgc tttaggaaga gactgtgcgg catcggcttc atcgaagagc
     3901 acctgccgct gaagattgct ttcttccaca aagtcgcgat caatcaatgt gagcgtgccc
     3961 actccggcac gggcaagaag actggccgcc gctgcgccgg tggccccaca gccgacaatg
     4021 gcaacgtggg cgcgggccag cttctgctgg ccttcttcgc cgattccggg ataaaggacc
     4081 tggcgggaat aacgttcggt gaattgcgtt aaaagcatcg gatgatttct cgcgtcctga
     4141 ttagtataaa agtgggtggt gtgctctggt tgcagcatgg acctgatggg caccgcaaga
     4201 ctgcatggag tgaagagagg ctgattggat tttcgctgca aatcctggtg gagaatttgc
     4261 tggatctgcc tcctggtctt tgcatggtgt gaagtagcac tggcgaaagc gcaggccaat
     4321 gaatcagccg gacaaacagg cgatgtgact ctgcgcacgt ggagtggcct gcagatagat
     4381 ggcatccaat tcaaaggcat agaagccgaa cggctggatc cattgccgca gaaactggcc
     4441 cagcaaccgg ggcagccgct tgatccggac aaggtgaggc aaagtctgcg ccgcctgtac
     4501 gaaaccggcc tgtacaaaac gatccaggta gagggagtca ggcacggaaa taccgtcacc
     4561 atcatttttg acggtacgcc aaacctcttt atcggccgca ttacggtgga aggggtcagg
     4621 gccgaacgcc tttccggagt gctggagcgc tcgacgcgta tgaaccctgg cactgttttt
     4681 acggatgaca aattgcagca ggctgaatcg caactgcggc agacgctgga agaaaacggc
     4741 tattacgaag caaagatttt tgccagcacg agtattgacc agccaaattc acaggtgaat
     4801 gtggccttcc agattgagtt gggcaagcag gcccgcgtgg gcaatgtggc ggtcacaggc
     4861 gacagcggaa tgagtacgga aaaattccgc aaaaccggga aattgaagga aggctccaaa
     4921 gttacacgga acacggtgag caacgcgctg acccggctgc gcaagaatta tcagaagaac
     4981 gacaggttgg aagcggccat taaattagat tcgcgggaat accggcctcc ggtgaaccat
     5041 ctggattatg gattcacggc caatcgcggg cctctggtga aaatacaggt ggaaggcaca
     5101 tccctgagca aagggaagat taaaaacctt gttcctgtct atgaggaagg cacggtagat
     5161 gaagatctgc tgaatgaggc cgaccgccgc atccgggact atttccagcg ccagagctat
     5221 ttcgatgcga aggtgacaca ttcggagcaa caggtagatc cgcgccatgc ggtgattacc
     5281 tttcatgtgg acctggggag caagcaccgt gttgaagcag tgcatatcaa aggcaataag
     5341 tattttgaca gcgatgtgat ccggcagcgg ttgagcgtga ccccggcaag tgttgtaaac
     5401 cggcatggaa tgtacagcta tgcgctggca caggcggacg ccagcagtat tgagggtctg
     5461 taccagagca acggatttac ggatgtgaaa gtcacgccag agatacagga cgtagatacc
     5521 gggcccaacg ggcagcgcct gaaaacagac ctgctgacgg tgacataccg gattgaagaa
     5581 gggccacaga cgcgactcgg ggagtatgaa attcatggcg cgcaaaagat tccgcttgca
     5641 caactgaccc cgttgctgaa tacgcagtcc gggcagccgt attctgcggc gaacataaat
     5701 aatgatcgcg acacgctgtt gagctactac cttagccacg gttttgataa agcgcaggtg
     5761 caggtgacgc agcgccggtc atctgccagg ccaaatacga tggatgtcgc catggacatt
     5821 accgaaggcg agcaagtgtt cgtgaaccgc gtgttgatct caggcctcca ctacacgcga
     5881 ccgagcacag tggaggaccg cgtactactg catcccggcg atccgttaga ccagacggcg
     5941 ctgctggata cgcagcgcaa attgtatgat ctgacactgt ttaatcaggt gaacacggcg
     6001 gtgcaaaatc ctacaggtga tgaactgcgc aagaatgttc tggtgcagtt ttccgaagcg
     6061 cgccggtggg acctgaatta tggttttggc tttcaggcgc agacgggaaa tccgcagaca
     6121 gagtgcaagg accttacagc ggaggagctg atcaagctgg ggattaatcc aagcaccttt
     6181 cattgcagtc ccaatggcaa gacaggtgtg agtccggcgt tcctgttcaa tgtgtcacgc
     6241 atcaaccttc ggggtacgga caaatccatt tcgttgaata cgacttatgg cacgctggag
     6301 cagcgtgtgc agatgatctt ttccaatccg catttgttcg acaatccaaa gtttgacgcg
     6361 atcctttccg ggggctacac gaatgcgcag gacgtgacta cgtatgctgc gtcgcgcctg
     6421 gaagcctcaa tgcgcgtaac aaaccatgtc aacaaaccaa acacgctgat ttatctctgg
     6481 agttaccgcc gcgtcagagt agacccgaac agcgtgcagg tggccccgaa tgagattccg
     6541 ctggtttcac agccggtacg cgtgggtggc ccgggcatta cgtggatccg cgatacgcgc
     6601 gtgcctactc cgctggatgc gacgaagggc acgtacaaca cggtccagga cacgtgggca
     6661 gattctcact atggatcgca ggcaaacttc aaccgtattg atgcgactaa ctccagctat
     6721 tacgtgtttg gaaaacagaa caaatgggtg gtggcgcgca gcacgcggct tggtgtggaa
     6781 cgctcctatg gagcagacaa gtatgagctg attccgctgc ctgagcgcct ctatgcgggc
     6841 ggcgcgcagt cacatcgtgg attcggcatc aactccgctg ggccgcgcga ttcattgacc
     6901 ggatttccca ttggcggcgc cggcgcattt ataaacagca ccgagctgcg catgccaaat
     6961 cccacgttgc catatgtagg caacagtctc ggatttgtgc tgttccatga catgggcaac
     7021 gtctttgaaa attcttctga tatctggccc agcatgtttc gcatcaagca accgcacagc
     7081 tatacctgca aggacaccaa tgaagctgac caatctaagc ctcatgagca ctcgagcact
     7141 ggcggcgccg gtacctgcag cttcaactat ttttcgcatg cagttggact gggtcttcgc
     7201 tatcacacgc cagtagggcc attgcgtctg gatttcagct acaacctgaa ccctccgatt
     7261 tatccgattc tgattgatta ttccggaggg ccggttccgc gcataggcca ggcaccgcat
     7321 ttcaatttct tcttcagcat cgggcaggca ttctgatggc atggcgcaca cagttcgggc
     7381 aacggacagc atgcgccata ctgtgtgtat gcctctgcac ccctgtctgg ccacagactt
     7441 cctctgcttc tgcaagcccg gttgttcttg atcgcgtaat tgccattatt aatggggatg
     7501 ttttgctgga aagcgatgtc cgcgaagaga tgcgtctttc tgtgcttcag cctatctcag
     7561 taccggcaaa ccagaacacg gaaatccgcg cggcccagcg gctgattaca cgaacgctga
     7621 ttctgcggca gatgaaagaa caagaggcga tcaactattc cgtgagcgat gaagatgtgc
     7681 gcaagagtct ggaggaactg cggcggcaga ttcctggatg tgggcctatg cactgcgcaa
     7741 cggaagcagg atggcaggca ttcctgcaga aaaacggact gaccgagcag gaggtggatg
     7801 cgcgatggaa ggagcggctg gagatactga aatttattga ccagcgtttc ggcgtgagta
     7861 tccacatcag ccgtcaggac atcgaaaatt attacaggaa aaattttcta cctgcctttg
     7921 caaagacaaa acagaagcct ccgacactgg agagcgttgc tcctcgtatc cgcgaaatat
     7981 tgctgcaaca acaggtgaat tcgcaactgc gggactggct ccagagcctg cgggagcagg
     8041 gaagcgtgca gatccttgat ccgaaatatg gccagagtac tgggaatgat gacgatacgg
     8101 gaggcggagc atgagcacac agcccgctct acctgctact gcgcgacgcc tttggctgcg
     8161 tgccggattg gtcgttgttg tggtgctcct ggctgtgatt ggcgggctta tctggtatgc
     8221 gtctacgccg cagtttgaaa acctggtccg ggagaagctg gttgccgttc tggagcagtc
     8281 cacgggcggg cgtgtagagt tgcaggcatt tcgatggtcg ctccggcacc ttgcttttga
     8341 ggctgacggt ctgaccatcc atggccttga agctgcaaac gaagctcctt atgtgcatgt
     8401 ggaacgcatt tatgtgcggg tcaggattct gtcattgttg ggacccaaaa agattgatct
     8461 gaattatctg gaagcagaca agcccgccat tcacctgatt atttatcccg atggctcgac
     8521 gaaccagcca aagccaaaga cacaacatca ggggaaatct gtaaaagatg agatttttga
     8581 ccttcaggcg ggcaggacgg aaatccgcga tggcgttgct ctgatcaatg aacgggcgat
     8641 accgttcaat tttgccgcaa atgatcttgg cgttgttgtg acgtatacgc ctgcgaatga
     8701 ccattatctg gcaacgttgc aggcgaccga tatggttgcg caacgtggag tgcgtccacc
     8761 gctgcgatcc cggctcgatc ttgccgctga tattggccgt aacacacttg atttgacgaa
     8821 atttgagctg cggacaggcc cgtcgtcgct ggaagctacg gctgcggtga aggactttgc
     8881 caatcctcac tggacattga tagcgcgcgg caaagtagat gtgcgtgagg tgatggtcct
     8941 ggagcctgtt gaaggtgttg acagcggcgt tgccacgctc gatctgaagg ggcagggaac
     9001 aaagacgcag tttctgatgg acggacttgt tcgcgtagcc ggggcggcct atcacacggg
     9061 cgcggtccac attacgggcg cgaacgcgga catggccctg catatgacac acgatgatct
     9121 ggcactcaca ggcattcgcg taaaactcaa tcgcggcggc gctgtggatg cggagatgcg
     9181 catcgcgcgt tggctggaac ctgcgcgcaa gggcagcatt cgcgccagga ttggcggttt
     9241 ttctctgcgt tcagtgatgg cgatggttgc gcctccgcat tatgaggacc tgggctttga
     9301 tacatgggtt gcgggcgata cattcgtgga ctggacggga aatgcgtctg atctgagcgg
     9361 cagggcaaat gtgacgctta caccttcaag acaaacaccg aatggcgaag tgccgctttc
     9421 gggaattgta gatgctactc atttccagaa aaacggcact gtggttgtga agcaattgca
     9481 ggcgcagact ccggcaacgc gctttgatgt ttcgggcggg ctgggagtgt atccactcag
     9541 ccgtccttct gcacttcagg tgaatgttca gaccacgaac ctggcagagt ttgacaaggc
     9601 tctctcgacg ttgggagtgg cctcacatgg aaagagtggt gtccaggcat tgcctgtgcg
     9661 gcttcaaggg caagcagagt tccacggtac agtttcagga acgctgagca gcccggatgc
     9721 gaaaggccac ctgaccgcag gcaatttcga tctgctgctg gctggacgca cgatccactg
     9781 ggactcgctt gtaacggacg ctgagtattc gccgcaacgg atcgcggtcc agcaggcgac
     9841 gctgacgcat ggcacaacga cagtccatgc atcaggtcaa ctccaggcgc accgtttgag
     9901 tgagcggcat tatgcgtttg atgatgaatc ggcaatccag gcagatcttc gggtgcagaa
     9961 cgccgagttg gctgatgtga ttgctatttc tggcaggaat cttccggaca cttccggtac
    10021 agtgactctg aatgcccatg ctggcggttc actctcaaac ctgaatggcg gcgggcatat
    10081 ttccatcgca ggcggaaaaa tttatggtga gccataccgc agcctgaatg ccgatctgcg
    10141 gtttacaggc gatgaggttg gcgcgacgaa acttacattt ctggaaaatg gcggtcaact
    10201 cacagcagaa ggcggctacc atgttcgcga taagcaattt cattttcatg cgcagggaaa
    10261 aggcttcgat ctgtcgcaca tagagcggct gcagaacaag cgcattcctg taacaggcca
    10321 actcgtcttt accgcgagcg gatcagggac tctggaatcg cccaatttgc aggtgaacgc
    10381 acatcttact catatcaccg tcggcaacga aatcaacgga gcagtggacc tggatgcgca
    10441 tacgggccat ggcgcgcttc tttacacaac tacaggacat ttaggacagg cactgttaca
    10501 aattaacggg cagactgcaa tcaccggcga tttccagacc caagcacagg cacagcttac
    10561 gaacctggac attgacccgt ttctgaagat gttcgaggtg gaaggggtgc agggacactc
    10621 ctcgattgcc gggttggtgt ctgtttccgg gccactgcgc gcgccccggc agat
//
LOCUS       DASRBV010000077         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_3930,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000077 DASRBV010000000
VERSION     DASRBV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..905
                     /locus_tag="VFA02_03200"
     CDS             156..905
                     /locus_tag="VFA02_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08883.1"
                     /translation="MFRSNRFYPVLAFALVFSAAFELRAQQTPPIVDQQPAQTAPQQQ
                     TPQERAEVLRQAQERVKARRRLRDQQTIKDTYSHKYEVYFGGGYLRFRPGNVLQHINE
                     AAWNVGITDYAYGKIGLTADVRGYYGTAYTGRNPYGVYQPSISQYTFMAGPTVRFFEG
                     VHWGWTAHVLAGVGHGNFGTGTGGLPPTLINLYPDGNSFNLAAGASVDYNLSPALALR
                     LTPSYLMTRYGSSTQNNLGWQGSVVYRWGRQ"
     gene            1088..1351
                     /locus_tag="VFA02_03205"
     CDS             1088..1351
                     /locus_tag="VFA02_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08884.1"
                     /translation="MAYRYIWWMNAADALERPERIVAQVMELGDWEDLQIVVHSLGDD
                     YLRRVIAQAEPGQFNERSWHYWHYRLHLAELGTVPPLPMRVFP"
     gene            1435..2025
                     /locus_tag="VFA02_03210"
     CDS             1435..2025
                     /locus_tag="VFA02_03210"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZU08885.1"
                     /translation="MVLYGGTAIALRLGHRTSVDFDFFKSGDLPGREAMLAHFPFLTG
                     AKTLQERPNTLTVVTVPIKNAEPVKVSFFGGISFGRVGIPQTTNDGVMEVASLDDLIG
                     TKLKVILQRIEAKDYQDIAAMLRYGVTLERGLSSARAMYGPAFQPSEALKALVYFHGG
                     DLDRLPHEDRKTLIEAVQHVRDLPQVFLRDRELTLR"
     gene            complement(2104..3528)
                     /locus_tag="VFA02_03215"
     CDS             complement(2104..3528)
                     /locus_tag="VFA02_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZU08886.1"
                     /translation="MPETRQEKDSLGLVEVPVRAYYGAQTARAVENYPVSGMRAHPVL
                     IRAFGMVKRAAAEANKELGLLDTKRADAIIQAAQEVIAGKWNAEFVVDVFQAGAGVSF
                     HMNTNEVIANRANEILGGKLGEYAFIHPNDHVNYGQSTNDVFPTGMRLATLLNLETLY
                     PVLDALAASFAAKGKEFDSVMKSGRTHMQDAVPIRLGQEFAAYGVAIAKGKQALQRAA
                     DSLHELGLGGSAVGTGINTHPDYRAKAIANLSRISGQRLVPAEDMRWAMQSNACMGEV
                     SAALRGIALEVIRISNDLRLMSSGPNTGFNEIHLPSLQPGSSIMPGKINPVMPELAAM
                     VSFQVIGNDTAVAYAVQAGQLELNVMMPTMAYNVLQSTTILANMLRQFNDRCVSGITA
                     NVARCNFYAQSTVSLATALNPYIGYAKAAEIVKESISTGKSIIEIARSKKLLSEKEIA
                     EILDPVKMTEPQPPLEAAKKRDRR"
     gene            complement(3615..>4460)
                     /locus_tag="VFA02_03220"
     CDS             complement(3615..>4460)
                     /locus_tag="VFA02_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZU08887.1"
                     /translation="EELRIELYKKVNELGIGAQGLGGLTTVLDVKVRDYPTHAANLPV
                     AMIPNCAATRHAHFVLDGSGPVALEPPSLDDWPKLTYDASSARRVNLDTVTREEVAAW
                     KPGEVLLLNGKLLTGRDAAHKRLTDMLNRGEKLPVDFTNRFIYYVGPVDPVRDEVVGP
                     AGPTTATRMDKFTRQMLEQTGLLGMVGKAERGPAAVEAIRDNGAVYLMAVGGAAYLVS
                     KAIQSSRVLAFEDLGMEAIYEFEVKDMPVTVAVDSNGTSVHETGPKEWRAKIQSQLAG
                     VPILA"
BASE COUNT          958 a         1278 c         1231 g          993 t
ORIGIN      
        1 tgcgcaagcg atttcccgat ttccattttc ctgcaaaccc gtctaaggcc atagaataga
       61 aggagcttta catctggaaa accggtctgc cttgttggcc cgatgcggtg ccccagaatt
      121 tttgttttct ccggcagtgg aggcatttga ggttcatgtt tcgatccaac cgcttttatc
      181 cggttctggc attcgcactt gttttttccg cggcatttga attacgggcg cagcagacac
      241 cacccattgt ggaccagcag ccagctcaga ctgctcccca gcagcagacc ccgcaggagc
      301 gcgccgaggt gctccggcag gcacaggaac gcgtgaaggc ccgccgccgt ctgcgcgacc
      361 agcaaacgat taaagacacc tacagccaca aatacgaagt ctactttggc ggaggttatc
      421 tgcgcttccg gccaggcaac gttcttcagc acatcaatga agccgcctgg aacgtgggca
      481 tcaccgacta tgcttacggc aagatcggtc tgactgccga tgtccgcggc tactatggaa
      541 cggcctacac gggccgcaat ccatatgggg tctaccagcc cagcatcagc cagtacacct
      601 tcatggctgg cccgaccgtc cgcttttttg aaggtgtgca ttggggctgg accgcccatg
      661 tcctggcagg tgttggacac ggaaacttcg gcaccgggac cggcgggctg cctccaacgc
      721 tgatcaacct ttatccggac ggaaattcgt tcaacctggc cgcaggcgca tctgtggact
      781 ataacctgag cccggccctt gcattacgcc ttacccccag ctatctgatg acgagatacg
      841 gcagctccac ccagaacaat ctgggatggc agggaagcgt ggtttatcgt tggggacggc
      901 agtagcttaa gggcgataac atattggctg ctgtaaaaac tctcaattgg ggtaaaggct
      961 tcagccaggc ggtaaagagt taaaaattag tttgacttcg gccgctgagg taagctgcaa
     1021 gtctatgagg agcgtcaatc caaccaatct ccccctgaca gatgaacaac tgcaggttct
     1081 caaagagatg gcataccgct atatctggtg gatgaatgca gcagatgctc tcgaacggcc
     1141 tgaaagaata gtggcacagg taatggagtt gggagattgg gaggatttgc aaattgttgt
     1201 ccactcgctc ggagatgact atcttcgccg ggtcatcgcg caagcagaac cgggacaatt
     1261 caatgagagg tcgtggcatt actggcatta ccgcctgcac ctggcagagc tgggtacagt
     1321 cccgcctctt cctatgaggg ttttcccgtg agcagtttcc aacccgtgtt ggatatactc
     1381 ccttccagcc aacgagcgct gtggccctca ttggctggaa gccgcaagtt tgggatggtc
     1441 ctttatggcg gcactgcaat cgcactccgg ctaggtcatc gcacatcggt tgatttcgac
     1501 ttctttaaat caggtgacct gcctggccgg gaagcgatgc tggcgcactt tccgttcctg
     1561 accggagcaa agacgctgca agagcgccca aataccttaa ctgttgttac tgtgccgatc
     1621 aagaacgcag aaccggtcaa agtatcgttc tttggcggaa tcagctttgg ccgggttgga
     1681 attcctcaga ccacgaatga tggggttatg gaagtagcat ccttagatga tctgatcggt
     1741 acaaagctaa aggtcattct gcaacgtatt gaagccaaag actatcagga tatagcagca
     1801 atgctcagat atggtgtaac cctggaacgt gggctttcat cagccagagc aatgtacggt
     1861 cccgcatttc agccgagcga agcattaaag gcgttggtat acttccatgg cggggatctg
     1921 gataggctac cgcatgaaga tcgcaaaacg cttatcgagg ctgtgcagca tgtccgggat
     1981 ctgcctcagg tttttttaag agacagggaa ttgaccctcc gctgagtagg caaaaactgc
     2041 aggaattgac tcagatcatc gcaaagggca cagagagctc gcaaaggatg tcaacagagc
     2101 caactagcgt ctgtctcgct tcttcgctgc ttccagcgga ggctgcggtt cagtcatctt
     2161 taccggatcg agaatctctg caatctcttt ctcgctcaat agcttctttg agcgcgcaat
     2221 ctcaataatg gattttcccg tagagatgga ctccttcaca atctctgccg ctttcgcgta
     2281 cccaatgtaa ggattcagcg ctgtagccag tgaaactgtg ctctgcgcat agaaattgca
     2341 gcgggccaca ttagctgtga ttccggaaac gcagcggtca ttgaactggc gcagcatgtt
     2401 ggcgaggatc gtcgtactct gcaggacgtt gtaggccatt gtcggcatca tcacgttcag
     2461 ctcaagctgt ccggcctgca cggcatacgc tacggcggtg tcgttgccga tgacctgaaa
     2521 actgaccatc gccgccagct cgggcatgac gggattgatc ttccccggca taatggacga
     2581 ccctggctgc aagctcggta gatggatctc gttgaatcct gtattcggcc ccgaggacat
     2641 gagccgcagg tcattcgaga tgcggatgac ctcgagcgcg atgccgcgca gcgcggcgct
     2701 aacctcgccc atacaggcat tcgactgcat cgcccagcgc atgtcttcgg caggaacgag
     2761 cctctggccg gagatgcgcg acaagttcgc aatggctttg gcacggtaat cgggatgtgt
     2821 attgatgccc gtgcctacgg cgcttccacc cagccctagt tcgtgcagtg aatcggcagc
     2881 ccgctgcaaa gcctgcttgc ctttcgcaat ggccacgcca taggccgcga attcctgccc
     2941 taaacgaatg ggcaccgcgt cctgcatgtg cgtgcggccg gacttcatca cgctgtcaaa
     3001 ctctttgcct ttggctgcga aagatgctgc cagcgcgtcg agcaccggat agagcgtctc
     3061 cagattcagc agagtggcca gacgcatccc ggtcgggaag acgtcattcg tggactggcc
     3121 gtaatttacg tggtcgttgg gatgaataaa ggcatattca cccagcttac cgcccagaat
     3181 ctcattcgcg cggttggcga tgacttcgtt cgtattcatg tggaagctga cacccgctcc
     3241 cgcctgaaag acatctacga caaactcagc attccatttc cctgcgatga cttcctgcgc
     3301 cgcctggatg atggcatcgg cccgttttgt atccagaaga ccaagctctt tgttggcctc
     3361 ggccgccgct cgcttcacca tcccgaaggc gcggatgagc acaggatggg cgcgcatccc
     3421 tgagacggga tagttctcaa cagcccgcgc cgtctgcgca ccgtagtaag cgcggactgg
     3481 tacttctact aatccgagag agtctttttc ctggcgtgtt tcgggcatag cagatattag
     3541 atgccctgaa acatatcagg atcaacactc gacatcctga ggcgcgaagg atgaagcatc
     3601 ccgatgtcgc gctttcaggc caggataggc acacccgcaa gctgtgactg tatctttgcc
     3661 cgccactcct tcggcccggt ctcatggacc gaggttccat tggaatccac tgcgacggtc
     3721 accggcatgt ctttgacctc aaattcgtag atggcctcca tgccgaggtc ttcaaaggcc
     3781 agcacgcgcg atgactggat ggccttcgag acaagatagg ccgccccgcc aacggccatc
     3841 aggtatacgg caccattgtc tcggatggcc tctactgccg ccggaccgcg ctcggccttg
     3901 ccaaccatgc ccagaagacc ggtttgctcc agcatctggc gcgtaaactt gtccatacgc
     3961 gtcgctgtcg ttggcccagc agggccaacg acctcgtccc gcacgggatc cacagggcca
     4021 acgtagtaga taaagcgatt ggtaaaatcc acgggcagct tttcgccgcg gttgagcatg
     4081 tccgtaaggc gcttgtgcgc ggcgtcgcgt ccggtcagca gcttgccgtt cagcagcagg
     4141 acctcgcccg gcttccacgc cgccacttct tcgcgcgtga ctgtgtcgag atttacccgt
     4201 cgcgccgagg aagcatcata ggtcagcttt ggccagtcat cgagcgaagg cggctctagt
     4261 gcgactggac ctgagccgtc cagcacaaag tgcgcgtgcc gtgttgctgc gcagttggga
     4321 atcatcgcaa ccggcaggtt ggctgcatgc gtcgggtagt cgcggacctt cacgtcgagc
     4381 accgtcgtca gtccgcccag cccctgcgcg ccgatgccca gctcgttcac tttcttataa
     4441 agctcgatgc gcagttcttc
//
LOCUS       DASRBV010000078         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_459859, whole genome shotgun sequence.
ACCESSION   DASRBV010000078 DASRBV010000000
VERSION     DASRBV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459859"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1522
                     /locus_tag="VFA02_03225"
     CDS             38..1522
                     /locus_tag="VFA02_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU08888.1"
                     /translation="MSHKLYRNLIGGEWVAARTGKTFLNTNPANKNDVVGEFQSSGAD
                     DVDAAVRAAAEAFKTWRLVPAPKRAEILYRTGHLLEQRKEQYAKEMTREMGKVLAETR
                     GDVQEAIDTAYYMAGEGRRLFGQTTPSELPSKFAMSVRMPVGVCGMIAPWNFPMAIPS
                     WKLFPALVCGNTCVIKPAEDTPLSTFNLVQTLMDAGLPKGVVNIITGYGPDAGAPLVE
                     HPGVRAISFTGSSEVGRIVGQTAAAHFKPCSLEMGGKNAMIVLDDANLDLALDGAVWG
                     AFGTTGQRCTATSRIILHKNIAAEFTEKLVTRAKALKIGDGLDESVEMGPQINEQQIE
                     TSTKYCEIAQQEGAKLLCGGKRLTEGAYANGTFFAPTVFSGVKPNMRIAQEEIFGPVV
                     ALIECGSFDEAIEIANGIRYGLSTALYSRNVNNAYRAMRDLEAGITYINAPTIGAEVH
                     LPFGGVKQTGNGHREGGTGALDFYTTWKAVYVDYSDKLQRAQID"
     gene            1538..2653
                     /gene="ald"
                     /locus_tag="VFA02_03230"
     CDS             1538..2653
                     /gene="ald"
                     /locus_tag="VFA02_03230"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZU08889.1"
                     /translation="MIIGVPKEVKDHESRVGITPAGVKALTEAGHKVLVETHAGELSA
                     FTDDEYQSAGAEIAGSAHNVWGQADMVVKVKEPIEKEYGFFREGLVLFTYLHLAPIPV
                     LTEKLLEKKVVAIAYETIRDKAGTLPLLTPMSEVAGRMSVQVGASYLEKEKGGRGLLL
                     GGVPGVPPANVCIIGGGIVGTNAAKIAIGMGAKVTIVDLNLNRLRELDDIFNGKVFTL
                     ASNSYNISRAVQEADLVIGGVLIPGAAAPKIVTREMVSKMKKGAVIVDVAIDQGGCIE
                     TAKPTTHSNPAYEVNGVVHYCVTNMPAAVPNTSTLALTNATFPYVMRLAQMGARKAIL
                     ADDGIRDGVNTYEGTLTCEPVAVSQKKSWKPVRELLA"
     gene            complement(2724..3095)
                     /locus_tag="VFA02_03235"
     CDS             complement(2724..3095)
                     /locus_tag="VFA02_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZU08890.1"
                     /translation="MATTTVTPETTDVKKAPITLTGKAVEKVREIMATQDPLPAGLRI
                     GVVGGGCSGFQYSMSFENQSGMMDKVFNFDGLKVFVDATSLMYLNGCTVDYVETLEAA
                     GFKFDNPNTKSTCGCGSSFNV"
     gene            complement(3255..3746)
                     /locus_tag="VFA02_03240"
     CDS             complement(3255..3746)
                     /locus_tag="VFA02_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZU08891.1"
                     /translation="MATSCAGTDSILLIMIASRKLAPAQWIATLLAFVALLWFAGCTS
                     RPQSDEQLKEQAAKTTEQVKQGAKQAAADAKVAAANAERKINDVAAGVKEGLKNGSPV
                     DTSININSASAGQLETLPGITRSRAERIIKGRPYSDPQDLVSKGILTQVQLDRISGQI
                     TAK"
     gene            3893..4165
                     /locus_tag="VFA02_03245"
     CDS             3893..4165
                     /locus_tag="VFA02_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08892.1"
                     /translation="MSIMLVLWIVWAVVTAFLLVLLAYRGTLTRYEEDQLFLDPAGSH
                     EEQQQNEIVQKVNRIQPLVRIATGATCILSATIIGVYVWDAIKQFQ"
     gene            complement(4223..5272)
                     /locus_tag="VFA02_03250"
     CDS             complement(4223..5272)
                     /locus_tag="VFA02_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZU08893.1"
                     /translation="MRIAATSAVYPPHYYTQQQVVGALLEFWGSSPEKAQVLERLHSR
                     TGVEGRYFSRRLEEYRALDTWGKANDVWIEVAGQLGEQSLDCVLKAAGLAREQLHAIF
                     FVSVTGIASPSIDALLVNRMGLSPNIKRNPIFGLGCVAGAAGLARAADYVRAYPDQVA
                     VLLSVELCSLTWQRDDISVANLISSGLFGDGAAAVLIAGEKTKLAGPRIIANHQVFYP
                     GTKDVMGWKISEKGFTIVLSPDVPKVVEENLGRDVDVFLAAHGLTRKDIGSWIMHTGG
                     PKILEATANSLGLKNGELAVSWEMLRRVGNLSSASVLVVLDEVMKRQRPPAGTRSILA
                     AMGPGFCAEMLLLEW"
     gene            complement(5511..6203)
                     /locus_tag="VFA02_03255"
     CDS             complement(5511..6203)
                     /locus_tag="VFA02_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08894.1"
                     /translation="MAGLAQGQVAPGPNVLPDAPDAQTQQQQASGSMEGKQTKRILGV
                     IPNFRSVSADEKLPPQSAKEKFITATQDSFDYSAFIFAGALAGISMAQNSYPEFRQGA
                     VGYGRYYWHTLADQAVENYMVEAAFPAALHQDSRYYTLGRGGIVKRTAYSFSRILITR
                     TDAGGETFNASEVIGAGAASGISSLYYPARERTWTKVGQHWLTNVGLDGMTFIFKEFW
                     PDINNAIFHQKQ"
     gene            complement(6314..>6635)
                     /locus_tag="VFA02_03260"
     CDS             complement(6314..>6635)
                     /locus_tag="VFA02_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU08895.1"
                     /translation="ALSLPQSYLDDRLKEYRERRDLLVKGLNDLGLECDPPAGAFYAM
                     PNVTKIHKESRKAAALLLEKAHVATIPGVVFGAQGEGYVRFGYSVPKPAIEAGLEALS
                     KFLK"
BASE COUNT         1443 a         1916 c         1878 g         1398 t
ORIGIN      
        1 ggtttggctt atttcggcat ctctcaaggc gaattttatg tcacacaagc tttacaggaa
       61 cctgatcggc ggcgagtggg tcgcggcaag gaccggcaag acttttctga ataccaatcc
      121 tgccaacaaa aatgatgtgg tcggcgagtt tcagtcctcg ggtgctgacg acgtggacgc
      181 ggcagtccgc gccgccgcag aggccttcaa aacatggcgg ctggtccccg cacccaagcg
      241 ggccgaaatc ctctaccgca ccggacacct gcttgaacag cgcaaagaac agtatgccaa
      301 agagatgacc cgcgagatgg gcaaggtcct ggccgagacg cgcggcgatg tgcaggaggc
      361 cattgatact gcctactaca tggccggcga gggccgccgc ctctttggcc agaccactcc
      421 gtctgagctg cccagcaagt tcgccatgtc ggtgcgtatg ccggtgggcg tctgcggaat
      481 gattgcaccg tggaacttcc ccatggccat cccttcgtgg aagctcttcc cggcgctggt
      541 ctgtgggaac acctgcgtca tcaagcccgc ggaagacact ccgctttcga ccttcaatct
      601 ggtccagaca ctgatggatg caggcctccc gaaaggcgtg gtcaacatca tcaccgggta
      661 tggccctgat gccggagcgc cgctggtcga gcatcccggg gtccgcgcga tctccttcac
      721 cggatcgagc gaagtgggcc gcatcgtggg ccagacggcc gccgcccact tcaagccgtg
      781 ctcgctcgaa atgggcggca aaaatgccat gatcgtcctt gatgatgcga acctcgacct
      841 ggcgctcgat ggcgccgtct ggggggcctt cggcaccacc ggacagcgct gcacggcaac
      901 cagccgcatt atcctgcaca aaaatattgc cgctgaattt acggaaaagc tcgtcacgcg
      961 cgcgaaggcg ctgaaaatag gcgatgggct ggacgagtcg gtcgagatgg ggccgcagat
     1021 caacgagcag cagattgaga cctccaccaa atactgcgag attgcgcagc aggaaggcgc
     1081 aaagctgctc tgcggcggta aacgtctgac cgaaggcgcg tatgccaacg ggacattctt
     1141 tgcaccgacc gtcttctccg gcgtgaagcc aaatatgcgc attgcgcagg aagagatctt
     1201 tggcccggtg gtggcgctga tcgaatgcgg cagctttgac gaagcgattg aaatcgccaa
     1261 cggcatccga tacgggcttt cgacggcgct ctattcgcgc aacgtcaaca acgcctaccg
     1321 cgcgatgcgc gaccttgaag caggcattac gtacatcaac gcgccgacca ttggggccga
     1381 ggtgcatctg ccctttggcg gtgtaaagca gaccggcaat ggccaccgcg agggcggtac
     1441 cggagcactc gatttctata cgacatggaa ggccgtctac gtggattatt cagacaaact
     1501 gcaacgggcg cagattgatt aaggagagta gcaagagatg atcatcggag taccaaaaga
     1561 agtaaaagac cacgagagcc gcgtcggcat cacgcctgcc ggagtgaaag ctctgaccga
     1621 agcagggcac aaagtcctcg tcgagaccca cgcgggcgag ctttctgcct ttacggacga
     1681 cgagtaccag tccgcgggcg cagagattgc cggctcggcc cacaacgtat ggggccaggc
     1741 cgacatggtc gtcaaggtca aagagccaat cgagaaagaa tatgggtttt tccgcgaagg
     1801 acttgtgctg tttacctatc tgcacctggc ccccattccc gtgcttacag aaaagcttct
     1861 ggaaaagaag gtggtcgcca tcgcttatga gaccatccgc gacaaggcag gtacactgcc
     1921 gctgcttacg cccatgtccg aagtcgcagg gcgcatgtcc gtccaggtgg gagcgtccta
     1981 tctcgaaaaa gaaaagggcg ggcgtggact gctgctgggc ggcgttcccg gcgtgccgcc
     2041 tgccaacgtg tgcattatcg gcggaggtat cgtcggcacc aacgcagcga agattgccat
     2101 cggcatggga gcgaaggtga ccatcgtgga cctgaacctg aaccgcctgc gcgagcttga
     2161 cgatatcttc aacggaaagg tctttaccct cgcctcgaac tcctacaata tttcccgcgc
     2221 tgtgcaggag gccgatctgg tcatcggcgg tgtgctcatt cctggagcgg ccgcgcccaa
     2281 gatcgtcacc cgagagatgg tctcgaagat gaaaaagggc gcagtgattg tagacgtggc
     2341 cattgaccag ggcggctgca ttgaaacggc aaagcccacg acgcacagca accctgcgta
     2401 tgaggtcaac ggtgttgttc attactgcgt cacgaacatg cctgccgctg ttccgaacac
     2461 atccacgctg gcgctgacca atgctacctt cccgtacgtg atgcgattgg cgcaaatggg
     2521 cgcaaggaag gccatcctgg ccgatgacgg cattcgcgac ggcgtgaata cctacgaagg
     2581 cacactgacc tgcgaacccg tcgccgtgtc gcagaagaaa agctggaagc cggtgcggga
     2641 attgctggcg taaaaacagt ccgatgaaag cagaaagggc gtgagtgatt cacgcccttt
     2701 tattctccca tgccgggtga tacttacacg ttgaaagacg agccacagcc gcaggtgctc
     2761 ttggtgttcg ggttgtcgaa cttgaagccg gctgcttcca gcgtctccac ataatccacg
     2821 gtgcagccgt tcaggtacat gagcgatgtc gcgtccacaa agaccttcag gccgtcaaag
     2881 ttgaagacct tgtccatcat gcccgactgg ttctcaaacg acatggaata ctggaagccg
     2941 gaacatccgc cgcccaccac gccaatgcgc aatcctgccg gaagcggatc ctgggtcgcc
     3001 atgatctcgc ggactttttc gaccgccttg ccggtgagcg taatcggcgc ctttttcaca
     3061 tccgtggttt cgggcgttac cgttgtggtt gccatgcggt tctccttgag cgttgctgct
     3121 gctttaagtt tacggcattt tcacaggtgg catcaatgag ggcccagttt gcttgtgtcc
     3181 gtgaaaacct ctccacaatt ggatgaatcc ccttgcccag gggattcatc gcgcccgaac
     3241 ggaccgcatc cggcttattt tgcagtaatc tggcccgaga tcctgtccag ttgcacctgg
     3301 gtcagaatgc ccttgctcac caggtcctgc ggatcactgt aagggcggcc tttgatgatg
     3361 cgctctgcgc gtgaccgcgt gattccgggc agagtttcaa gctgcccggc tgaagccgaa
     3421 ttgatattga tggacgtgtc aacaggactg ccgtttttca ggccctcttt cactccggcg
     3481 gccacatcgt tgatcttgcg ctcggcgttg gcagcagcga ctttcgcatc ggcggcggcc
     3541 tgctttgcac cttgctttac ttgttcagtg gtctttgccg cctgttcttt caactgctcg
     3601 tcagactggg gccgggaggt gcatccggcg aaccacagca gggcaacaaa ggcaagcagg
     3661 gttgctatcc attgcgcggg ggccagttta cgggaggcga tcataatcag caatatggaa
     3721 tctgttccag cgcaagaggt tgccaaatca acttcacgtc acggaaaaag gtactattca
     3781 actcaggtat ttagggctaa tatgatttgc gaacggcctc acttaccccg ggccttccgt
     3841 tcggcccgct cgtacctggg accggctcta aatcgaatcc ttgaggtgct ccatgagcat
     3901 catgcttgtt ctgtggatcg tctgggccgt cgtcaccgcc ttcctcttag tcctccttgc
     3961 ttatcgggga acactgaccc gctatgagga agaccagctc tttctcgacc cggcagggag
     4021 ccatgaagag cagcagcaaa atgaaattgt gcagaaggtc aaccgcattc agccgcttgt
     4081 gcgcatcgcc acgggcgcaa cctgtattct gtccgcaacc attatcggcg tatatgtctg
     4141 ggacgctatc aagcagttcc agtaaagtgt gttcctgttg atttccggag cgcccagatg
     4201 tcccggcgct ccggctttct tcctaccact ccagcagcag catctctgcg caaaaccctg
     4261 gccccatggc ggccagaata cttcgtgtcc cagcgggagg gcgctggcgt ttcatcacct
     4321 catccagcac caccagcacc gatgcggaag acaggttacc aacgcggcgc agcatttccc
     4381 atgaaacagc aagctcgccg tttttcaggc cgagcgagtt tgccgttgct tccagaatct
     4441 tcggccctcc tgtgtgcatg atccatgagc cgatatcttt gcgggtaagc ccatgcgccg
     4501 caagaaatac gtccacatcg cgtcccagat tctcctcaac cactttggga acatcgggcg
     4561 acagcacaat ggtgaagccc ttttcagaga tcttccagcc catcacatct ttcgtaccgg
     4621 gataaaagac ctgatggttc gctatgatcc gcggcccggc gagtttggtc ttctcgcctg
     4681 caatcaaaac agcggccgcg ccgtcgccga agagccctga cgaaatcagg ttggcgacag
     4741 aaatgtcatc gcgctgccag gtgagcgagc acaactcgac agaaagcagc acggcaacct
     4801 gatcaggata agcgcggacg taatcggccg cccgggccag tcctgctgcg ccggcaacac
     4861 aacccaggcc aaaaatggga ttgcgcttga tattcggtga aagccccatg cgattgacca
     4921 gcagagcatc tatcgaagga ctggcaatgc cggtgacaga cacaaagaag atggcatgga
     4981 gttgttcgcg ggctagtccg gcagctttca gcacgcagtc cagggactgt tcgccaagct
     5041 gccccgcaac ttctatccag acatcgttgg ctttacccca cgtatccagc gcgcgatact
     5101 cttccagcct gcgcgagaaa tatctgcctt ccacgcctgt gcgcgagtgc aggcgctcca
     5161 atacctgcgc cttttccggg ctgctccccc agaactcgag cagcgcaccg actacctgct
     5221 gctgtgtata gtaatgcgga ggatagacgg cagacgtggc ggcgatgcgc aatggacagg
     5281 acctcctgtt caagattaga ggcaatcagg agcgcgcgga ggtcattctt ttgtctgctt
     5341 gatgtaatgg aaaaggggaa aagcaccatt tacccgctgt gctcacttcc agatcatgga
     5401 accgctgctt ccaattctgt gcgagggcaa gaaagactgc atatttggcc aacgaatctc
     5461 tgtgaccgtg cagccggatc cgtcctctga ccgcgacaag actgtagttt ctattgtttc
     5521 tgatggaaga tcgcgttgtt gatgtctggc cagaattcct tgaagatgaa ggtcattcca
     5581 tccagaccga cgttggtaag ccagtgctgg cctacttttg tccaggtgcg ctctctggcc
     5641 ggataataca ggctggaaat gccggaggcc gcgcccgcgc caatcacttc tgaggcatta
     5701 aacgtctcgc cccccgcatc ggtgcgtgta atcaggatgc gtgaaaacga gtaagctgtc
     5761 cgctttacga tgccgccgcg acccagcgtg tagtagcgcg aatcctgatg cagggcggcc
     5821 gggaaagcgg cctccaccat ataattttct actgcctgat cagccagggt gtgccagtag
     5881 tagcggccgt atccaactgc cccctggcga aactcagggt aagaattctg cgccatgctg
     5941 attccggcca atgctccggc aaaaatgaat gcggagtaat cgaagctgtc ctgtgttgcc
     6001 gtaataaatt tttcttttgc cgactgcgga ggcagcttct catcggcgga aacagaacga
     6061 aaattgggga tgactcccag gatgcgcttg gtctgcttac cttccatgct gccggaagcc
     6121 tgctgctgtt gcgtctgcgc gtcgggtgca tcgggaagga cattcgggcc cggtgcgacc
     6181 tgtccttgtg cgaggcctgc caaagaaaag acaaaaattc ctgctgtaat tttctttatc
     6241 catgcaaaaa agtgcatgaa gtttagacga tacccggcag tgttcgggtg cagagaattt
     6301 gcccttcaca tggctatttc aggaattttg acagcgcctc taatcctgct tcaatggcgg
     6361 gcttaggcac tgaataaccg aaacgcacgt atccttcacc ctgcgcgccg aaaaccacgc
     6421 cgggaatggt ggccacatgc gctttttcaa gaagcagcgc agcagctttc cggctttcct
     6481 tgtggatttt cgtaacattg ggcatggcgt aaaacgcgcc cgcgggaggg tcgcattcga
     6541 gccccagatc attcaacccc tttaccagaa ggtctcgtct ctcacgatat tccttcaggc
     6601 ggtcgtccag gtagctttgc ggcagcgaga gcgct
//
LOCUS       DASRBV010000079         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_209593, whole genome shotgun sequence.
ACCESSION   DASRBV010000079 DASRBV010000000
VERSION     DASRBV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209593"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VFA02_03265"
     CDS             <1..1578
                     /locus_tag="VFA02_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU08896.1"
                     /translation="EAHHNVRIKDSAIIAAAELSHRYISDRFLPDKAIDLVDEAAASL
                     AIQIGSVPTEIDQLEREATSLEIEKAALKRENDENSKKRLEEVERELAALREKVTGLR
                     ARWQKERDAITRLAELKKKVEALRFEAEEQTRQGNLERAAQIQYGELPKLEAELKQLN
                     AAQDGNANAQRMLKEEVDEEDIARIVSKWTGIPVSKMLEGEVKKLIQMEARLRERVIG
                     QDEALAIVANAIRRSRAGLSDPKRPIGSFIFLGPTGVGKTETARALAEFLFDDEQAMV
                     RIDMSEYMEKHAVARLIGAPPGYIGFDEGGQLTEAVRRRPYSVVLFDEIEKAHPDVFN
                     VLLQVLDDGRLTDSKGRTVDFKNTVIIMTSNLGAHILNAESLSNDVGFEKARQQVMDV
                     LRQSFRPEFLNRVDDIVIFRPLGEEQLTHIIDLRLADLKKLLADRHISITLTDAARHM
                     LFTQGYDRAYGARPLKRAMQKLVQDPLAMKILDGEILHGDEVKIDADTRTGKMTFEVT
                     SRAGVPLEGHHMAIGRA"
     gene            1645..2202
                     /locus_tag="VFA02_03270"
     CDS             1645..2202
                     /locus_tag="VFA02_03270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZU08897.1"
                     /translation="MSTPIQNIPVRRINGEETTLAEFAGKVVLVVNVASKCGLTPQYA
                     GLENLYRKYRERGFVVAGFPSNDFAGQEPGTNEEIQQFCSLNYNVDFPLFEKITVVGA
                     AKHPLYQALIEARPKARSTAIVPFAQDLMKYGIQPNPEPEILWNFEKFVIGRSGEVVA
                     RFSPDTKPDDTGLTSVIEKELAKPA"
     gene            complement(2223..2984)
                     /locus_tag="VFA02_03275"
     CDS             complement(2223..2984)
                     /locus_tag="VFA02_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU08898.1"
                     /translation="MGMRVITRPDGSSAAEPCECQQERRIARLVDRAKVPPRYRHCTL
                     QSFEVGRHSHESLRAAFLMADKFVTAYPLETDGKGLLFTGSMGVGKTHLAIGLLLALI
                     TERGAQGLFCDYRELLKQIQESYNPSTHITELQVLKPIFDAEVLLIDDLGYIKPTEWV
                     WDTVALILNARYNAKRTTLITTNYPNLPAAGVQEGQEAIPVSRERRMAVSAMREETLG
                     DRIGERMRSRLMEMCVEVKMEGKDLRQSVKRARFS"
     gene            complement(3063..4148)
                     /locus_tag="VFA02_03280"
     CDS             complement(3063..4148)
                     /locus_tag="VFA02_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZU08899.1"
                     /translation="MKVFIAVALEFLCTFAGQAQTQTTPPSTPQAVPANVPQINTSYI
                     DAQGTAHITRVVPVPQTVSTEAQKFLARPISDVAQSPPVAQQRAMTDAWQAKAGEAYR
                     QVYPATVSSATMAGVPVRIITPPVIGAGKSDRVLLNLHGGGFMVDSGSLTETIPIANI
                     TQTKIVAALYRLAPENPFPAAVDDAIAVYKELLKTYKPKNIAIYGTSAGAILTAETVA
                     KIKQLGLPMPGALGIFSGMGDFSRPGDSQAIFGLEGLSGHLAVPAQGVQLPEYVAKTD
                     AKDPVLSPVYSDLRGLPPTLFVTSTRDLLLSGTSILHQAFLKAGVDAQLIVFEGLPHA
                     FWNNVDLPESRDADEIMAHFFDTHLGK"
     gene            complement(4248..5873)
                     /locus_tag="VFA02_03285"
     CDS             complement(4248..5873)
                     /locus_tag="VFA02_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZU08900.1"
                     /translation="MDETTSAVISPGLSPAETLLDRKIKKSPGLIGSLIFGGVLLAGL
                     GYAGFHLASDISGVHVVSAWPYVLLGLALLIALGFEFVNGFHDTANAVATVIYTHSLE
                     PHVAVVWSGTWNLIGVLTSSGGVAFSVLSLLPVELVLQVGRGAGFAMVFALLIAAILW
                     NLGTWALGLPASSSHTLIGSIIGVGIANQLMNGRNGTSGVDWGQVSNVFRALLISPLV
                     GFVCAALLLVVLKFLIKDKALYESPKGDAPPPFWIRALLILTCTGVSFGHGSNDGQKG
                     MGLIMLILIGVVPTAYALNHAVGFNQVQDFAAVSEQAANVLSHYVSPDAVMGDARSDL
                     TEYIRTKEYTSNTPLAARQLAIDISHEVQLYKSLARVPASQQQNVRNDMYVEGEALRL
                     MAKSAQPGFTPQETAVITNYRKHLDTSTKFIPGWVKVAVALALGLGTMVGWKRIVVTV
                     GEKIGKEHLTYAQGASAEIITAATIIAADQFKMPVSTTHVLSSGVAGTMMANGSGLQL
                     STVRNIALAWVFTLPAAALLAGCLFWMLRQFIH"
BASE COUNT         1324 a         1595 c         1934 g         1193 t
ORIGIN      
        1 gaggcgcacc acaatgtgcg catcaaggac tcggccatca ttgcggcggc ggagttgtcg
       61 caccgctaca tctcagaccg ctttctgccg gacaaggcga ttgacctggt ggacgaggcg
      121 gcagcatcgc tggccatcca gattggctcg gtaccgacag agattgacca actggagcgc
      181 gaagccactt cgcttgagat tgaaaaggcc gcgctcaagc gcgagaacga tgagaattca
      241 aagaagcgtc tcgaagaggt cgagcgcgaa ctcgccgccc tgcgcgagaa ggtcaccgga
      301 ctgcgcgcgc gctggcaaaa agagcgggac gcgattacgc gtcttgctga attgaagaaa
      361 aaagtcgagg cactgcgctt cgaggctgaa gaacagacgc ggcagggcaa tctggagcgt
      421 gcggcgcaga tccagtacgg cgagttgccg aagcttgaag cggagctgaa gcagctgaac
      481 gctgcgcagg acggcaatgc aaacgcacaa cgcatgttga aagaagaagt ggatgaggag
      541 gacattgccc gcattgtgtc gaagtggacc ggaattcccg tctccaagat gctggaaggc
      601 gaggtaaaaa agctcatcca gatggaagct cggctgcgcg agcgcgtgat tgggcaggac
      661 gaagcgctgg cgattgtggc gaatgcgatt cgccgttcac gcgcaggact gagcgatccg
      721 aagcggccga ttggctcgtt catctttctg gggccgacgg gcgtgggtaa gacggaaacc
      781 gcgcgcgcgc tggccgagtt tctgtttgat gatgaacagg cgatggtgcg cattgatatg
      841 tctgagtaca tggagaagca cgctgtcgcg cggctgattg gcgcgcctcc gggctacatt
      901 ggctttgatg aaggcggcca gttgacggag gcggtgcggc ggcgtccgta ctcggtggtg
      961 ctcttcgatg aaattgagaa ggcgcatccg gacgtgttca acgtgttgct gcaagtgctc
     1021 gatgatggcc gtctgacgga ctcaaagggc cgcacggtgg actttaaaaa cacggtcatt
     1081 atcatgacgt caaatctagg cgcgcatatt ctcaacgcgg aatcgctgtc aaacgatgtt
     1141 ggctttgaaa aggcgcggca gcaggtgatg gatgttctgc ggcagagctt ccgtccggag
     1201 tttctgaacc gcgtggatga catcgtgatc ttccgcccgc tgggcgaaga acagttgacg
     1261 cacatcattg acctgcggct tgctgatttg aaaaagctgc tggctgaccg ccacatctct
     1321 atcacgctta ccgatgcggc acggcacatg ctctttacgc agggctatga ccgcgcatat
     1381 ggagcgcgtc cgctgaagcg cgcgatgcag aagctggtcc aggacccgct ggcgatgaag
     1441 atcctcgacg gcgagattct gcacggcgac gaagtgaaga tagacgcgga cacgcgcacc
     1501 ggcaagatga cgttcgaggt caccagccgc gccggagtac cgctggaggg ccatcacatg
     1561 gccatcggga gggcataaga ccgtgcggcg gaaagggtcc gggaacaggt agtcccgggc
     1621 cccttcacgt tacaattgca tccaatgagc acaccgattc aaaacatccc ggtccgccgc
     1681 atcaacggag aagaaaccac tttggcagag tttgcgggca aagtggtcct ggtggtcaat
     1741 gtagcttcca aatgcggact gacaccgcag tatgcggggc tggagaacct gtaccggaaa
     1801 taccgtgagc gcggctttgt ggttgccgga tttccctcca atgacttcgc cggacaggag
     1861 ccgggcacga atgaagagat ccagcagttc tgctcgctga attacaacgt ggatttcccg
     1921 ctttttgaga agatcacggt tgtgggcgcg gcgaagcatc cgctatatca ggccttgatt
     1981 gaagcacgac ccaaggcccg cagcactgcg attgttccgt tcgcgcagga cctgatgaaa
     2041 tatggcattc agccgaatcc ggagccggaa attctgtgga actttgaaaa gttcgtcatt
     2101 ggacgaagcg gagaggtcgt tgcgcgtttt tcgccggaca ccaaaccaga tgacacggga
     2161 ctgacgtcag tgattgagaa agaactggcg aaaccagcct gatggatttc ccgggatgcg
     2221 cttcaggaaa agcgcgcccg ctttacggat tggcgcaggt ctttgccttc catctttact
     2281 tcgacgcaca tttccatcag gcgcgacctc atgcgttcgc cgatgcggtc acccagtgtt
     2341 tcttcgcgca tggccgacac ggccattctg cgctcgcggc tgacgggtat ggcttcctgt
     2401 ccttcctgaa cgccggcagc gggaagattg gggtagtttg tcgtgatgag cgtggtgcgt
     2461 ttggcgttgt agcgggcgtt caggatgagg gccaccgtgt cccacaccca ttcggtcggt
     2521 ttgatgtatc cgaggtcgtc aatgagcagg acttcggcat caaagatggg tttgagcacc
     2581 tgaagctctg tgatgtgggt cgagggattg taggactcct ggatctgctt cagcaattcg
     2641 cggtagtcgc aaaagaggcc ctgcgcgccg cgctcggtaa tcagcgccag cagaaggccg
     2701 atggcgaggt gggtcttccc tactcccatg gaccctgtaa acaacagacc tttgccgtcg
     2761 gtttccagag ggtaggcggt gacaaatttg tcggccatca ggaatgctgc acgcaatgac
     2821 tcatgcgaat gccgcccgac ttcaaagctc tgcagagtgc aatgcctgta gcggggaggc
     2881 acctttgcgc ggtctacgag ccgggcgatg cggcgctcct gctggcactc gcaaggctcg
     2941 gcggcgctgc ttccatcagg gcgcgtgatg acgcgcatgc ccattccatc acatttcgca
     3001 caaacttcgg tcattgctct ggaaccagta tcgcagggcg tggtgtgcat atccagtccg
     3061 cgctactttc cgagatgcgt gtcaaagaag tgcgccatga tttcgtctgc gtcgcgcgac
     3121 tcgggcaggt ccacgttgtt ccagaaagcg tggggcagtc cctcgaagac aatgagttgg
     3181 gcgtccactc ctgccttcag gaaggcctga tgcagaatgg aggttccact caggagcagg
     3241 tcgcgggtgc tggtgacgaa gagggttggg ggcagtcccc ggagatcaga ataaacaggg
     3301 gagagcacgg ggtctttggc atcggttttg gcgacgtatt ccggaagctg cacgccctgg
     3361 gccgggacgg caagatgtcc tgaaagccct tcgaggccga agatggcctg cgagtcaccg
     3421 ggcctgctga agtcgcccat gccggaaaag atgccgagcg cgccgggcat gggtagtccg
     3481 agctgtttga tttttgccac tgtttccgcg gtgaggatgg cgcctgccga ggttccgtaa
     3541 atcgcaatgt tctttggctt ataggtctta agcaactctt tgtagacggc aatggcgtcg
     3601 tccactgcgg cgggaaacgg gttttccggc gcaagacgat agagcgcagc cacgattttc
     3661 gtctgcgtga tgttggcaat gggaatggtt tcggtcaggg agccggagtc caccataaag
     3721 ccgccgccat gcagattgag gagcacacgg tcgctcttcc ctgccccgat gacaggggga
     3781 gtgatgatgc gcacgggtac tccggccatg gtggcgctcg aaacggtggc gggatagacc
     3841 tggcggtagg cttctccggc cttggcctgc catgcatccg tcatggcgcg ctgctgggcc
     3901 acaggcgggg actgggccac gtcggaaatg ggccgcgcga gaaacttctg cgcctcggtg
     3961 ctgaccgtct gaggtaccgg gaccacgcgc gtaatgtggg ctgttccctg cgcgtcaata
     4021 tagctggtgt ttatctgggg gacattagct ggaacggcct ggggagtgga agggggtgtc
     4081 gtctgtgtct gggcctggcc ggcaaaggtg cagagaaatt caagagcaac cgcaatgaaa
     4141 actttcatgc tcgccaatac accacagaaa atggaaaaga tgcaagcgat cgggaaggcg
     4201 gcccagcaca agccacaata ttctgtaaaa gccaggccct tcagacatca gtggatgaac
     4261 tggcgcagca tccagaagag gcaaccggcg agtaaggccg ctgccggaag cgtaaagacc
     4321 caggccaggg cgatgttccg gaccgtggag agttgcaggc cgctgccgtt ggccatcatc
     4381 gttccggcaa cgcctgatga gaggacgtgc gtagtagaca cgggcatctt gaattggtca
     4441 gcggcgataa tcgtagcggc ggtgatgatc tctgccgaag cgccctgcgc atacgtcagg
     4501 tgctctttgc cgatcttctc gcctacggtc accacgatgc gcttccaacc gaccatggtg
     4561 cctaatccca gagccagggc cacagccact tttacccagc ctgggatgaa tttcgtagaa
     4621 gtatcaagat gcttcctgta gtttgtgatg acggcggttt cctgaggagt gaagccgggt
     4681 tgcgcgctct tcgccatcag acgcagggcc tcgccttcca catacatatc gttgcgcacg
     4741 ttctgctgct ggctggcggg gacccgggcg agcgacttat agagctggac ttcgtgcgaa
     4801 atatcaatgg ccagttggcg cgcagccaga ggcgtgttcg aagtgtactc ttttgtgcgg
     4861 atatattcag tgaggtcgct gcgggcatcg cccatgactg catcgggcga gacataatga
     4921 ctcagcacat tggctgcctg ttctgagacg gcggcgaaat cctgcacctg attgaagccg
     4981 acagcgtggt tgagggcata ggcggtgggc accacgccga tgagaatgag catgatgagt
     5041 cccatgccct tctgtccgtc attggagccg tgaccgaagc tcacgcctgt gcaggtgagg
     5101 atgagcagcg cgcgaatcca gaacggagga ggcgcatcgc cctttggtga ttcgtagagc
     5161 gctttgtctt tgataagaaa tttgaggacc acaagcagca aggccgcgca gacaaatcca
     5221 acgagcggcg agatcagcaa tgcgcgaaag acattcgaga cctggcccca gtccactcca
     5281 ctggtgccgt tgcggccatt cattaactgg ttggcaatgc ccacgccgat aatggacccg
     5341 atgagcgtat gcgaactgga ggcaggaagc cccagcgccc aggtgcccag gttccacaga
     5401 atggcggcaa tcagcagcgc aaaaaccatg gcgaaacccg cgccacggcc tacctgaagg
     5461 accagttcga cgggcagcag cgagaggacc gaaaaggcca cgccgccgga ggaagtgagc
     5521 acgccaatca ggttccatgt tccagaccag acgacggcga cgtgcggctc cagtgaatgc
     5581 gtatagatga cggtggcgac agcgtttgcg gtgtcgtgga atccattgac gaactcgaac
     5641 cccaacgcga tgagcagtgc aaggccaagc agcacgtatg gccaggcgct gacgacgtgc
     5701 acaccggaga tgtcactggc gagatggaac ccggcatagc caagtccggc aagcagaact
     5761 ccgccgaaaa tcagcgaacc aatgaggcct ggcgattttt tgattttccg gtcgagaagg
     5821 gtttctgcag gagacaatcc gggggaaatg acggcagagg ttgtttcatc catagttctc
     5881 ggctccgata gggcacgtgt gagagcaacc tatcagggcc ggtggagagt atgtgtaaaa
     5941 atgcgaggga aatctttatg aatttttggt aaatgagtca ggtgtttgtg gtctttcaag
     6001 cgagggcctg cgaacccaca ttccggagga gtggtacatc ctggca
//
LOCUS       DASRBV010000080        12038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_665572, whole genome shotgun sequence.
ACCESSION   DASRBV010000080 DASRBV010000000
VERSION     DASRBV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665572"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="nadA"
                     /locus_tag="VFA02_03290"
     CDS             <1..706
                     /gene="nadA"
                     /locus_tag="VFA02_03290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZU08901.1"
                     /translation="LPLTYMNSTAAIKAFCGERGGLVCTSSNAGRAFEWAFARAEKIL
                     FLPDQHLGRNTAYAMGIPLNEMAVWDPYQINGGLTPDRLRRAKIILWKGHCSVHQRFL
                     PEHVDKVRAKYPGIQVIVHPECRWEVCQKADALGSTERLISLVSDAPEGTMFAIGTEI
                     HLVNRLAKEFAPKNKKIITLDDTGCLCTTMYRISPQHLAWALENLVEGRVVNQIRVRD
                     DVKHWARVALDRMLEV"
     gene            703..1131
                     /locus_tag="VFA02_03295"
     CDS             703..1131
                     /locus_tag="VFA02_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HZU08902.1"
                     /translation="MKTFTLDEAQSLLPVLESLLKRAIEGKKAAEEIEAQLQQLRHRI
                     FLSGGMMVDILSVVRQKAEAEKFVRQAKDAVAEMDAIGVQVKDLDTGLLDFPCMLQDE
                     IVLLCWKMGESRIDFWHTVDAGFRGRQPVDERFGRTKKPN"
     gene            complement(1205..2002)
                     /locus_tag="VFA02_03300"
     CDS             complement(1205..2002)
                     /locus_tag="VFA02_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZU08903.1"
                     /translation="MSVLLGNGDGTFRNSINSKVLGEPANNTLNKQVYAVDVNHDGKA
                     DLIGDWGVAIGHGDGTFSKPVPFPSSVLPIAQLAVGDINLDGNLDIVTGNFLQGTIYT
                     FLGDGTGKFSLTHTEKLNYTTPWLQALTLADMNGDHIPDLVYVYSATPSSGSYDRVVV
                     ELGNGSGTFTFASGVRIDYNGYGYNFLLVDDFNRDGKPDVMVLTLSSSGKSALLRGTG
                     EGALSTAQDVSLQLYPGAAIDLNGDGAPDIVGANGPGVDRVLNTGAH"
     gene            complement(2015..3430)
                     /locus_tag="VFA02_03305"
     CDS             complement(2015..3430)
                     /locus_tag="VFA02_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZU08904.1"
                     /translation="MQRRVFRLVLAAAAGLVCVLAAQPSFSQTSFPKQFVTPQYLNLP
                     NPAYNNLVGVGDLNGDGRPDILYAYSELLATGNGTFQTIALPSNQYLGGGQLADVNGD
                     GKLDVVIPVPADVECDQYPDGTWYCNYNSYAGLNVYFGNGDGTFRSGPILNLNEYASG
                     TYTMAISDVNGDGKPDVLLSFQGVPEADGTEQSFVLLNNGDGSFHSVSNPFFSSVLTT
                     GDFNGDGKTDFVAGLNSVTIYFGKGDGTFTAGPLNGNGDFYDLTSATAADFNHDGHLD
                     LAIASPQSGVYLLLGDGSGRFTIQHTSTLSATQVTAADLNHDGYEDIIAGLRGFIAIF
                     TNHNGTLSYPQVFDMVQPAQPLSFGIADFNHDGYLDVVAGNSIAYGAPGAMFHAPVIT
                     QSAGAHSVVTGDFNRDGIQDVAVTSNDGWVTILPGSGKGYLNGGPRYYTGIRAGLITA
                     GDVNGDGIADLLVVRDGYYSP"
     gene            complement(3672..5132)
                     /locus_tag="VFA02_03310"
     CDS             complement(3672..5132)
                     /locus_tag="VFA02_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08905.1"
                     /translation="MKNIALAALLASAFVCASAQDGHVHWTRNGAPTETTITGGPWTL
                     EQSGAAVGLKSAGYCDANGNQAGNPGTERMQPYYFPVVRGHGKYLQGYFDWRPKDIDE
                     AVVAAYSNDAGQSWYFQQKALELRTTCPDQVQKYPNGQKEPGPDPNNSDNGDDDGQGH
                     QFVISIGGHTYLYTLDRANNHIDSDDLYIHELDPQPGLPLNGAPAINDGPTDETSSEP
                     EIAFHTTGLLNPDGILGVVPGSWPLKIIYEQKILNGDITGNTALPASQLCSTYWSNYY
                     ANNPYGTKTNDDITYLRLAETSDGIHFKDLGILQGLNNPGDVGLTGTRWLATAGTILK
                     LDHDRYGLFFSGGGCVDGDSDAFHYIGYAESHDLLHWNVVYGINNPVLSTTPITMTVG
                     SNGTPLTSSGPGTTITVPANTPVAGNTLGFFAGRVYAPSGAIYNHDTLTIIFAGYHTQ
                     KPKYGLGDYRTIGRLSLHASQPLTHDDDNWRDNDDR"
     gene            5323..5649
                     /locus_tag="VFA02_03315"
     CDS             5323..5649
                     /locus_tag="VFA02_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU08906.1"
                     /translation="MAKNDLQGSLDLLVLKTLSQTGPLHGYGIVLHIERASDALLNVE
                     EGSLYPALHRMEQSGWLRSEWATTETGRKAKYYKLTPSGRKQLEEAEKSFEQLVRGVR
                     AVLRYV"
     gene            5656..8391
                     /locus_tag="VFA02_03320"
     CDS             5656..8391
                     /locus_tag="VFA02_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08907.1"
                     /translation="MMPIFRRIANLFSRLRLERDTEAELQSHIEMRMEDNMAAGMSEE
                     EARRDALLRFGNPVVTKERVTAMDVPLILESLWRDLRYGVRQLVRRPGFTATAVLTLA
                     LGIGATSAIFTLFDQVLLRMLPVERPKELVRLEWRGSFSGSASGFGGDVSNYFSYPMY
                     KDLRERNQVFSGMLAAMRTSIGVAWHGQAEEKDAEIVSGNYFQVLELRPSLGRLFTSA
                     DETQKNANAVVVLSYNYWRTRFTASRDVIGQTLLINGHPFTIIGVAPENFQSAIGGYR
                     PGLFVPVTMSDIAMPWRASSNSLENHLSIWITVVARLKPGMSIARAEAGLAPLWHSLR
                     AQELTLYKSASQSFKERYAEKSRLMVKDDSLGFSPNRIDLKMPLMILMSMAGLLTAMC
                     ALNVATLLLLRAAARAREMSMRYALGAKLARIAAQLLVEGGLLGICGAVSGLVLTPLV
                     ARILVRLMTNSEPGSEPYSAAVDIRVLLFTLALSLVVSLLFSIAPVLHFLRPNLANAL
                     RQSTGTATKGPQIFRKIAVGVQIALSVLLLGGAGLFVRTLNHLRQQNVGFDTKHLVTF
                     GLNPTESGYDEAHTSPIVTTALDTIRKVPGVEMAAATTDPELAGDQEWNGYAIQGHKH
                     VEGERTDFENPWITPGYFATLKQPLLAGRDFTLLDANGAPKVAIVNLAFAKRFYGSAQ
                     NALGRMLGAADDHPHFDITIVGVVGDIKHQNLREDMGPAVYTPYFQKGHPAGVQIYIR
                     TTLEPEKVEASIRQAIHNLDSTLVVGDMRTMDVQVDISAFTERALAMLAISFAVLAMM
                     LAAVGLYGVLAYSTQSRTREIGVRLALGAQRRTVILLVVREMAWITVLAILIAVPSTA
                     ALARLFRSQLYGVTTFDPVTLASAVALAGVMLVLAAALPARRAASVNPVEALRNE"
     gene            8444..10177
                     /locus_tag="VFA02_03325"
     CDS             8444..10177
                     /locus_tag="VFA02_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZU08908.1"
                     /translation="MAVFRSSSAALIAAIICIAGHGGSALAQQGSGVQKSPSLPAISQ
                     RDQTLIESVEKAYQAGLSNYRDGHTTAARSNFDYAVDLILRSGIDIKGDPAISEEFDH
                     IVDSINTLEMDALRENGPQMAQQHPEDAPVDVANDVTFPVDPKVRAQAEAELKTTQSD
                     LPLVINDYVASYINFFSNTAKGHNTIVGALTRAGRYKNMIERILKEEGVPQDLIYQAV
                     AESGFRPQAVNPHSGAGGMWQFMPYGPYGLSRTGWYDERFDPEKATRAYARWIKQLYD
                     QLGDWYLAMAAYDWGAGNIQRAVERTGYADFWELYRRNNLPAETKNYVPIILAVTIMA
                     KNPKQYGLTDLTPDPPLMDDTVTTNYAIDLRLVADIVNAPLQEIVALNPSLLRMTTPP
                     DEPYDLHLPPGTKALFEKRIAEVPEDKRRYWRFRVLTPGESLEQVARQYHVSASEIAF
                     VNQLDSGSDLSGVDSLVIPVAPAAAPSSLRSSTYKTQRGDTLVTVADRFGVTVDQLRR
                     WNHLRGNTVTAGRRLYVAEPAHIASSSRHKKASSRSERTASQRTGKGAKEVHADTKAA
                     TPAHHHAKTSR"
     gene            complement(10225..10986)
                     /locus_tag="VFA02_03330"
     CDS             complement(10225..10986)
                     /locus_tag="VFA02_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08909.1"
                     /translation="MKDTAGKTTAGSGRKKAPLFAGLFCLPGAACYFFFAAFFVAFFT
                     AFFVAFLAAFFAGAFLAAAFFAAFFTAKLVLPRLVDFLLLALFFLTTDFFVALLPPRA
                     PLLGLAADFFAAFLAGAFFAAFFAGAFFAAFLAAFFGAAFFFAAGSADPGAAASPLST
                     SSAVAAGSSVSGRSSSSSSRSSSSEEIEMLTSSSSSSPKPSSTSSGSLWSKSLSSCAK
                     SLRSSCMKCPPAAAPEFSVLSQRARKVSAFPVRGL"
     gene            complement(11040..11375)
                     /locus_tag="VFA02_03335"
     CDS             complement(11040..11375)
                     /locus_tag="VFA02_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08910.1"
                     /translation="MELIRYLGAIHGVQAWLAYFNSDLSFAKSFNVTEHQNIQFRVSA
                     FNFLNHPLPQLGQGQDVNLHMSCVQSSSSAMGCDEGGTNVNTSTTGNVLYKAAQQHRY
                     MELSLKYNF"
     gene            complement(11465..>12038)
                     /locus_tag="VFA02_03340"
     CDS             complement(11465..>12038)
                     /locus_tag="VFA02_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU08911.1"
                     /translation="AKALEAVRRARRNGLHVTCEVAPHHFVLNEEAVGEYNTHAKMNP
                     PLRCEADRQAMLQGILEGSVDAIATDHAPHAAHEKEQEFERAPNGITGLETALGLTIA
                     YLHEKHKLPLSRVIALLSTQPAAVLNLKGRGSLAVGSFADVVIFDPKKQWTFHAADSK
                     SKSKNTPFDGWKLTGKVAVTISEGRIAYRG"
BASE COUNT         2779 a         3229 c         3367 g         2663 t
ORIGIN      
        1 tctgccgctc acttatatga attcgactgc ggccatcaaa gcattctgtg gcgagcgcgg
       61 tggactggtc tgcacctctt ccaatgctgg acgcgccttt gagtgggcgt ttgcgcgggc
      121 ggagaagatc ctgtttctgc ccgatcagca cctgggacgc aatacggcat atgccatggg
      181 gattccgctg aacgaaatgg ctgtgtggga cccgtaccag atcaacggcg gacttacgcc
      241 tgaccgtctg cgcagagcga aaatcattct ctggaaaggc cactgctctg tgcatcaacg
      301 ctttcttcct gagcacgtgg acaaagtacg cgcaaagtac cccggaattc aggtgattgt
      361 gcatcccgag tgccgctggg aggtctgcca gaaggccgat gcgcttggct cgacagagcg
      421 tttgatcagc ctggtcagcg atgctccgga aggcacaatg tttgccatcg ggacggaaat
      481 ccatctggtg aaccgcctgg caaaggagtt cgcgccgaag aacaaaaaaa ttatcactct
      541 cgacgacacg ggctgtctct gcactacgat gtaccgcatc agcccgcagc atctggcttg
      601 ggcgcttgaa aatcttgtcg aaggccgcgt tgtaaaccag atcagggtcc gcgatgacgt
      661 gaaacactgg gcgcgcgtag cgctcgaccg tatgctggaa gtatgaagac gttcaccctg
      721 gatgaggcgc agtcgttgct acctgttctc gaatcgctgc tcaagcgtgc cattgaaggc
      781 aagaaggcag cagaggagat cgaagcacag cttcagcaac tgcgccatcg catttttctt
      841 tccggtggca tgatggtaga catcctctcg gtcgtgcggc agaaggcgga agcagaaaaa
      901 tttgtgcgtc aggcaaagga cgccgttgct gagatggatg ctatcggcgt gcaggtgaaa
      961 gacctcgaca ccggactgct cgactttccc tgcatgttgc aggatgagat cgtcctgctg
     1021 tgctggaaga tgggcgaatc ccgtattgac ttctggcaca cagtagacgc gggtttccgt
     1081 gggcgccagc ccgtggatga gcgctttgga cggaccaaaa agccgaattg agaggtccag
     1141 gtaggcgaag gcccaacaac cgcagatggg cccttcgcca cactggatga aagcggaaca
     1201 ttcttcaatg cgctccggta ttcagcacgc gatctacgcc tggaccattg gccccaacga
     1261 tatcaggcgc gccatctccg ttcaggtcta tggccgcgcc ggggtacaac tgtaatgaca
     1321 cgtcctgagc tgtgctgagt gccccttctc ccgtgcctcg cagaagggct gattttccag
     1381 aactggagag ggtaaggacc attacgtcgg gttttccgtc acgattgaag tcatctacca
     1441 gaagaaagtt atagccatag ccgttatagt caatgcgtac tcctgacgca aaggtaaagg
     1501 tccctgagcc attgcccagc tcaaccacaa cgcgatcgta agatccgctg gatggcgttg
     1561 ctgaatatac gtacacgagg tcaggaatat ggtccccatt catgtcagca agagtcagtg
     1621 cctggagcca tggcgtggtg tagttgagct tttcggtatg ggtcaggctg aattttcctg
     1681 tgccatctcc aagaaaggta tagatcgtac cttgcaggaa atttcctgtc acaatatcca
     1741 ggttcccatc aaggttgatg tcccctaccg ctaactgtgc aatgggtaaa accgacgagg
     1801 gaaaaggaac cggctttgaa aatgttccgt ctccatgtcc gatggccacg ccccaatcac
     1861 caataaggtc ggctttgcca tcatggttca catccacagc gtaaacctgc ttgttcaatg
     1921 tattattggc aggctcgccc aataccttcg agttaatgga attacggaat gttccgtccc
     1981 cattgccaag caggacactc aagtcactgg gatactatgg gctgtaataa ccatctcgga
     2041 ccacgagcag gtctgcaatg ccatctccat tcacatctcc tgctgtaatc aggccagctc
     2101 gtattcctgt gtaatatcga gggcctccat tgaggtatcc tttgcctgag cctggaagaa
     2161 tcgtgaccca cccatcattg ctggtcacag caacatcctg aatgccatca cggttgaaat
     2221 cgcccgtcac gactgaatgc gctcccgcag actgagtaat aacaggcgcg tggaacatcg
     2281 cgccgggagc cccataagca atactgtttc ctgccaccac atcgagataa ccatcatgat
     2341 taaagtccgc aatgccgaag gaaagaggct gtgcaggttg caccatatcg aacacctgcg
     2401 gataggacaa tgtgccgtta tggttggtga atatcgctat gaatcctcgc aaacctgcga
     2461 tgatatcttc ataaccatca tgattcagat cagcagcagt gacctgggtt gctgacaacg
     2521 ttgaagtgtg ctgtatcgtg aaccggcctg agccatcccc caggagcaga tagactccag
     2581 attgaggcga tgctattgcc aaatccagat gaccatcgtg gttaaagtcc gctgctgttg
     2641 cagatgtaag gtcataaaaa tcgccattac cgttcagtgg tccggcagta aatgtcccat
     2701 cgcctttgcc gaaataaatg gttacgctgt taagcccggc aacaaagtct gtcttgccgt
     2761 ccccattaaa gtctccggtg gtcagcacac tggagaaaaa ggggttcgat accgaatgaa
     2821 agctcccatc accattgtta agcagaacaa aactctgttc cgttccatcc gcctcaggca
     2881 ccccctggaa actcagcaat acatccggct tgccatctcc atttacatcc gaaatggcca
     2941 tggtataggt tccacttgca tactcgttca gattaagaat tggaccgctt cggaatgtgc
     3001 catctccatt tccgaaatag acattcaggc cagcatagct gttgtaattg cagtaccagg
     3061 taccatcagg atactgatcg cattccacgt ctgccgggac cggaatgaca acatcgagct
     3121 taccgtcccc attcacatcc gctaattggc caccgccaag gtattgattc gatggaagtg
     3181 caattgtctg gaatgtacca ttccccgtgg caagcaattc actataggca taaaggatat
     3241 ctgggcggcc atcgccattc aggtccccta ccccaaccag gttgttgtat gcaggattag
     3301 ggagattcag atactgcggt gttacaaatt gcttaggaaa ggatgtttga ctaaatgaag
     3361 gttgagcagc aagaacgcat acgagccctg cggctgctgc aaggacaagg cgaaaaaccc
     3421 gtcgttgcaa ggggcacccc caattcgcgc tgaatcatat tcgatggtgc atacgctcta
     3481 ctatgggtag gtattctcat ctcatatttc gcgattgaga aatgcggcag agagcagacg
     3541 ggctgaacat cggaaaagcg gtaccgaatc cgggtcttgc attttcaccc tgttgcacgg
     3601 ttccggtaca acagggtgaa agcagctatc aactcatctc atcaagaacg tggagttgaa
     3661 aaggacagac cttagcgatc atcgttgtcg cgccagttgt catcatcgtg cgtgagcggt
     3721 tgggatgcat gcaggctgag acggccaatg gtacgataat cgcccagtcc gtactttggt
     3781 ttctgggtgt gatatccggc aaagataatt gttagtgtgt catggttgta gatggcccca
     3841 ctgggcgcat aaacgcggcc tgcaaagaac cccagcgtgt tcccggcaac cggagtgttc
     3901 gccggaactg tgatcgtggt gcctgggcca gacgaagtta gcggtgtgcc gttggagcct
     3961 accgtcatgg tgatgggcgt agtcgaaagg accggattat tgattccata gaccacgttc
     4021 caatgcaaca ggtcgtgcga ttcggcataa ccaatgtagt ggaaagcatc agagtcgccg
     4081 tccacgcaac cgccaccaga gaagaacagg ccgtaacggt cgtgatcgag cttgaggatg
     4141 gtgcccgcag tcgccagcca gcgcgtaccc gtcaggccca catctcctgg attattcagt
     4201 ccctgtagga tgccgagatc cttaaagtgg attccatcgg aggtttccgc gaggcgcaga
     4261 taggtgatgt catcgttggt cttcgttcca tacgggttgt tcgcatagta gttcgaccag
     4321 tacgtgctgc aaagctgtga agcgggaagc gcggtattgc ccgtgatgtc gccgttcagg
     4381 attttctgct cgtagatgat cttcaacggc cagctcccag gcacaacgcc aagaataccg
     4441 tcaggattca gcagacctgt ggtatggaag gcgatttccg gttcgctgct ggtctcatcg
     4501 gttggcccgt cgtttatggc aggggcgcca ttcagaggca atccgggctg cgggtcgagt
     4561 tcgtgaatgt acaggtcatc ggaatcaatg tgattgttgg cgcggtccag cgtgtagaga
     4621 taagtgtggc ctccgatgga gatcacgaat tgatggcctt gtccgtcatc atcgccgtta
     4681 tcgctattgt tcgggtcagg accaggctct ttctgcccat tgggatattt ctgtacctgg
     4741 tcggggcagg tggtgcgcag ttcgagtgcc ttctgctgga aataccagct ttggcccgca
     4801 tcattggagt aggcggcgac cacggcttcg tctatgtcct tcggacgcca gtcaaagtag
     4861 ccctggagat actttccgtg tccacgcaca accgggaaat aatagggctg cattcgttct
     4921 gttccaggat tgcctgcctg gttgccattg gcgtcgcaat agcctgccga cttgagtcca
     4981 acggcggcgc cggactgttc cagtgtccag gggccgcctg taatcgtggt ttcggtgggt
     5041 gctccgttgc gcgtccaatg gacatgcccg tcctgggccg acgcgcacac aaacgcagat
     5101 gcaagaagtg ctgcaagtgc aatgtttttc attgttttcg tctcctgcct cgcctgaaat
     5161 ggaagaaagg caatgaacag gaataacgga cgctcatgaa caagtggtga attttctgtg
     5221 acaacatcat gaaagcctgt ttcggcagca gttttttgca ttgacatacc tgtttgtcta
     5281 ggtatagtct ctctctctat accaagacgt ataggtataa gcatggcaaa aaacgacctt
     5341 cagggaagcc tcgatctcct tgttctcaag acgctctcgc aaacaggccc gctgcatggg
     5401 tacggcatcg tgctgcacat cgagagggcc tctgacgcat tgctgaacgt tgaagaaggc
     5461 tcgctctatc cggcgctgca ccgtatggag caaagcggat ggctgcgctc cgagtgggcg
     5521 acaactgaaa ccggccgcaa ggcgaagtat tacaagctca ctccctctgg gcgtaagcag
     5581 ttggaagaag cggaaaagag cttcgagcaa ctggtcagag gagtgcgggc cgtgctccgc
     5641 tacgtgtgaa ggtgaatgat gcccattttt cgacgcatcg ccaatctctt ctcgcgtctg
     5701 cggttagagc gtgatacgga ggccgaactt cagtcgcaca ttgagatgcg catggaagac
     5761 aacatggccg caggcatgtc cgaggaagag gcgcggcgcg acgctctgct gcggtttggt
     5821 aatcccgtcg ttacaaagga gcgcgttact gccatggatg ttccactcat acttgaaagc
     5881 ctctggcgcg atcttcgcta tggagttcgt cagcttgtaa gaaggcctgg attcaccgcg
     5941 accgcagtgc tgacgctggc attgggcatc ggggcaacat cggccatctt tacgctcttc
     6001 gatcaggtgc tgttgcgcat gttgccggtc gagcgtccga aggaactggt gcgactggag
     6061 tggcgcggct ctttctccgg ctcggcgagc ggcttcggcg gcgacgtcag caactacttc
     6121 tcgtatccca tgtataagga ccttcgtgag cgcaatcagg tcttcagcgg aatgctggct
     6181 gctatgcgca caagcatcgg tgtggcatgg catggtcagg cagaagaaaa ggacgctgag
     6241 atcgtcagtg gcaattattt ccaggtcctg gagctgaggc cctctctggg cagattattt
     6301 acatccgcgg atgaaacaca aaagaacgcc aatgcggtcg ttgtcctcag ctataactac
     6361 tggcggacac gtttcaccgc ctcccgcgat gtgattggtc agacactgct catcaatggg
     6421 cacccgttca cgattatcgg cgtagcgcca gaaaattttc agagcgctat tggcggctat
     6481 cgtccaggcc tctttgtgcc ggtaaccatg tcagacattg ccatgccctg gagggcttcc
     6541 tccaatagtc tggaaaatca cttgtccatc tggattacgg tggtggcacg gctgaaaccg
     6601 ggaatgagta ttgcacgtgc agaggcagga cttgctccgc tgtggcattc actgcgtgct
     6661 caggaactca ctctgtacaa gtcagcgtcc caaagcttca aagagcgcta tgccgaaaaa
     6721 tcccgtctca tggtgaagga tgattcactt ggcttttcgc caaaccgcat agatttgaaa
     6781 atgccgctta tgattttgat gagcatggca ggactgctga ctgccatgtg cgcactcaat
     6841 gtggccacgc tgctgctgtt gcgtgccgcg gcgcgtgcaa gggaaatgtc tatgcgctac
     6901 gcgctcggcg caaagcttgc gcggattgct gctcaactgc tggtggaagg cggcctgttg
     6961 ggcatttgcg gggcggtgtc tggcctcgtt ctgactccac ttgttgcccg gattttagtc
     7021 cggctgatga ccaactccga acctggcagc gagccgtact ccgctgccgt ggatattcgc
     7081 gtgctgctct ttacccttgc gctttcattg gtggtcagtc tgctcttcag cattgctcct
     7141 gtgctgcatt tcctgcggcc caatctggcg aatgctttgc gccagagtac aggcacggcc
     7201 accaaaggcc cacagatttt ccgcaaaatt gccgtcggtg tgcagattgc gctcagcgtt
     7261 ctgctgcttg gtggcgcggg actctttgtc cgcaccctga atcatctccg tcagcagaat
     7321 gtcggtttcg acacaaagca tctggtgacc tttggcctta atccgactga gtctggctac
     7381 gatgaggccc atacttcgcc catcgtcact actgcgctgg atacaatacg caaggtcccc
     7441 ggagtagaaa tggccgcggc aacaaccgat cctgagcttg ccggagatca ggaatggaac
     7501 gggtatgcca tccaggggca caagcacgtt gagggcgaac ggacagactt tgaaaatccg
     7561 tggatcacac ccggctattt tgcgacttta aagcagccgc tgctggcagg ccgcgatttc
     7621 actctgctgg acgcgaatgg ggcaccgaag gtggcgattg tgaatctggc atttgccaaa
     7681 cggttctatg gctctgcaca aaatgcgctg gggcgcatgc tcggcgcggc cgatgatcat
     7741 cctcatttcg atatcaccat cgtaggcgtg gtgggtgaca taaagcatca gaacctgcgc
     7801 gaagatatgg gaccggcagt ttacacgccg tatttccaga aaggtcatcc ggcgggtgtg
     7861 cagatttaca tccggacgac gctggagccg gaaaaggttg aagcctccat ccggcaagcc
     7921 atccacaatc tcgattccac gctcgtggtc ggcgacatgc gcaccatgga cgtgcaggtg
     7981 gacatcagcg cctttaccga gcgcgcgctg gcgatgcttg ccattagttt tgcagtgttg
     8041 gcgatgatgc tggccgccgt cggtttgtat ggagtgctgg cttactccac ccagagccgc
     8101 acgcgcgaga tcggtgtccg gctggccctg ggcgcgcaac ggcgaacggt cattcttctg
     8161 gtggtgcgcg aaatggcctg gattacggtg cttgccatcc tcattgcagt gccttccacg
     8221 gcggccctgg cgcggctctt tcgcagccag ctttatggag ttaccacctt tgacccagtc
     8281 acactggcat ctgccgttgc cttagcagga gtgatgctgg tcctcgcggc cgccctgcct
     8341 gcccgccgcg ctgcatcggt caatccagtg gaggcgctgc gcaatgaata gaagaatatg
     8401 acgcatcccg tggcgagcca ctacaataaa gcagtacatt tacatggctg ttttcaggtc
     8461 ttcatctgcc gctctaattg cggccatcat ttgcattgcg ggtcacggcg gcagcgcgtt
     8521 ggcgcagcag ggaagtggcg tgcaaaaatc tccttcgctt ccagccatct ctcagcgcga
     8581 ccagacgctg attgagagcg tggaaaaggc ctatcaggca gggttaagca attatcgcga
     8641 cggacatacc actgccgcgc gctccaattt cgactacgcc gtggacctga ttctccgcag
     8701 cggcattgat atcaagggcg atccggccat cagcgaagaa ttcgaccaca tcgtagactc
     8761 catcaatacc ctggagatgg acgctctgcg cgaaaacggt ccgcaaatgg cgcagcagca
     8821 tccggaagat gctccggtgg atgtcgccaa cgatgtgacc tttcctgtgg accccaaggt
     8881 ccgcgcccag gctgaagccg agttgaagac cacgcagtct gaccttccac tggtcatcaa
     8941 tgactacgtt gccagctaca ttaacttttt cagcaacacc gccaaaggcc ataacacgat
     9001 cgtcggcgca ctcacacgtg cgggccgcta caaaaacatg atcgagcgca tcctgaaaga
     9061 ggaaggcgtg ccgcaggacc tgatctacca ggccgttgct gaatccggct tccgcccgca
     9121 ggccgtcaat ccgcactcgg gagcaggagg catgtggcag ttcatgccat atgggcctta
     9181 tgggctttcc cgcaccggat ggtacgacga gcgctttgat ccggagaagg ccacacgggc
     9241 ctatgcccgc tggatcaagc aactctatga tcaactgggc gactggtatc tggccatggc
     9301 tgcgtatgac tggggtgcag gaaatatcca gcgcgctgtg gaacgcaccg ggtatgccga
     9361 tttctgggag ctttaccgtc gcaataatct ccccgccgag acgaagaact atgttccgat
     9421 tattcttgct gttaccatca tggcgaaaaa cccaaagcaa tacggactga ccgatctcac
     9481 gccggacccg ccgctgatgg atgataccgt tacgacaaac tacgcgattg atcttcgtct
     9541 tgttgccgat atagtgaatg ctccgttgca ggagattgtt gcgctgaacc caagtctgct
     9601 gcgcatgact acgcctccag atgagccgta tgatctgcat ctgccgccgg gaaccaaggc
     9661 gctgtttgag aaacgcatcg ctgaggtgcc tgaagacaag cgacgttact ggcgcttccg
     9721 cgtccttaca cccggagagt cgttggagca ggtcgcaagg caatatcatg tctctgcctc
     9781 tgagattgca tttgtgaacc agcttgattc cggcagcgac ctcagcgggg tggattcgct
     9841 cgtcattcca gtagctccgg ctgccgcgcc ttcatcattg cgcagcagca cttacaagac
     9901 gcagcgcgga gacacgctcg taacagttgc cgaccgcttt ggagtcaccg tagaccagtt
     9961 gcggcgctgg aaccacctgc gcggaaacac agtaacggca ggccgcaggc tttatgtcgc
    10021 agaacccgcg cacatcgcga gctcttcgcg gcataagaaa gcatcgtcac gcagtgagag
    10081 gaccgcatca cagcgtacag gcaagggtgc aaaagaagtg catgcggaca cgaaggctgc
    10141 aacgccggcg caccatcatg caaaaacgag caggtgaagc tgaataaata cttgctttca
    10201 ttggaaatgc gttgcatcct gttgctataa tccgcgcaca gggaaagcgg atactttgcg
    10261 cgccctctgt gatagaactg aaaattcagg cgccgcagcc ggaggacatt tcatgcagga
    10321 tgaccgcaaa cttttcgcgc aggacgacaa cgactttgac cacaacgaac cggaagaagt
    10381 agacgacggt tttggtgaag atgaagaaga ggaagtaagc atctcgattt cttctgatga
    10441 tgacgacctt gaagacgaag aagaggagcg accgctcact gaagaaccgg ctgccactgc
    10501 cgaagaagta gagaggggcg aggctgctgc acctggatct gcactcccgg cagcgaaaaa
    10561 gaaggccgct ccgaagaagg cagccaaaaa agctgcaaag aaagcgccag cgaaaaaagc
    10621 tgcgaagaaa gctccggcca agaaggccgc aaagaaatct gccgccaaac ccagcagggg
    10681 cgctcgcggc ggcagcaagg ccacgaaaaa gtccgtggtc agaaaaaaga gcgccagcag
    10741 taaaaaatca accaaacgag gtaaaactaa tttggcagtg aagaaagcag caaagaaggc
    10801 agcggccaag aaggcccctg caaagaaagc agccaagaag gcaacgaaga aggctgtaaa
    10861 aaaggcaacg aaaaaagcag cgaaaaagaa gtaacaagct gcaccaggca agcaaaagag
    10921 ccctgcaaaa agcggggctt ttttgcgtcc ggaacctgct gttgttttcc ctgctgtgtc
    10981 cttcaaaaaa aaaaaagggg accccttcgg gtccccagtt cgggaggcaa gcaaaccccc
    11041 taaaagttgt atttgagaga cagttccata tagcggtgct gttgcgctgc tttatacagt
    11101 acattgcccg ttgtggatgt attcacgttt gtgccgccct catcgcagcc catagcgctt
    11161 gacgagcttt gtacgcagct catatgcaga tttacatcct gtccctgacc caactggggc
    11221 agcgggtgat tgaggaagtt gaaagccgag acacggaact gaatgttctg atgctcggtt
    11281 acgttgaagc ttttcgcaaa tgacaaatcg ctgttgaagt acgcgagcca tgcttgaaca
    11341 ccatgtatag ctccaagata gcggatcagt tccacctacc ctagtacttc ctgctccact
    11401 gccactttgc agtgattgac atgtcgcgac atgtacagga gagcttttaa agggaatttt
    11461 ctgatcagcc gcggtaggca atccggcctt cactgatggt gacagccact tttcctgtca
    11521 gcttccagcc atcgaagggc gtgttctttg attttgattt tgaatcagcg gcgtggaagg
    11581 tccactgctt cttcgggtcg aagatcacta catcggcaaa cgaaccaaca gcgaggctgc
    11641 cgcggccctt caggttaagg acggcagcgg gctgcgtact cagcagcgca attacgcgtg
    11701 agagcggcaa cttgtgcttt tcgtgcaggt aagcgatggt gaggcccagt gccgtttcaa
    11761 gtccggtgat gccgttgggg gcgcgctcga actcctgctc tttctcatgc gcggcatgag
    11821 gagcatggtc tgtagcaatg gcgtccaccg atccttcgag gatgccctgg agcatggcct
    11881 gacggtcggc ctcacaacgc agaggcgggt tcatcttggc atgcgtgttg tattcgccga
    11941 ccgcttcctc attcagcaca aaatggtgcg gtgcgacctc gcaggtgacg tgcagcccat
    12001 tgcggcgcgc gcggcggacg gcttcgagtg ccttcgcg
//
LOCUS       DASRBV010000081        13090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_459964, whole genome shotgun sequence.
ACCESSION   DASRBV010000081 DASRBV010000000
VERSION     DASRBV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459964"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFA02_03345"
     CDS             <1..856
                     /locus_tag="VFA02_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZU08912.1"
                     /translation="EGERNPERKSGTRLHDRIVNAPNYPQKLSGFMITDVMQAVSYLR
                     TRPDVDPSRIAVLGYSMGSFVSALAGAVDPRIKVLVLSGGGDLDGPGGYWENSGKPMC
                     QSVPWKSLSVLGDKAAVVYALNQLRGPTFIMNGTKDTVVDIPGKHEQSFFDDLAHRTE
                     AITGTRKNIFETYFIPDASHRPNWVTRRAALWLQSKLHFPNWTAKTIMAMPETHISEW
                     AAKNHVDMDRAYLSEDKEGGIMALGSDVPNVPRTQLNVFTDQEFQEQKSRLVYQAWTA
                     NVLAAASE"
     gene            1576..5151
                     /locus_tag="VFA02_03350"
     CDS             1576..5151
                     /locus_tag="VFA02_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU08913.1"
                     /translation="MSKKLCNHHLCKTHFCWLFAAVLFLCSGTEAILPQTSSGTISGR
                     VVDQSGGAVTTADVRLINQWTGTMVRATVQPDGGFTFPDVQPGTFTVDVRANGYKEYL
                     KKDLVLSSAERLSAGTLVLEIGSVSQNVTVTAATTPVQTQSAEVSGLLDTQQLDNLLA
                     VGRDFMSMVRTIPGVVGGGANSLGTSGTPTINGLRNVNNSGNIDGVNGSPRGGDKIDT
                     PPNLDAIQEVKVLTANYQAEYGAGTAGPVINLVTKSGTQQFHGTAYYYVRNEAFNANN
                     WFNNYNNLPRPQYRYNDVGGNIGGPIYWPGHFNTNRNKLFFFFSMEYLPNKTPDGLKK
                     YMVPTALERQGDFSQTPVQGKVSPNPATDYINIKKPGADPGTCPASGTGGNHSGCYPG
                     NVLPANVINRQIQALLNVMPLPNFNNRAVSGGNYNYITNYTGNNPVNQEIFRIDYNPS
                     DKIHMFFRGEFMTVNDDSYSSPANKLPWLMRVNYQTTHPNLAYDMTYAFSPTLLNEIT
                     VGTSGFGETQLYNKSDLLKATKSSNGYNIGQLYPGNNPMNLFPAVSFGGISNAATFGW
                     DSRFPMYDRTRQYSLTDSLTKVLGAHNLKFGINLATDHYLQAHSSSGIPEGSFAFDRN
                     VNNPNDSNYAFANALQGLFNSYSEPTARSDYNPRIYIIEWYGQDQWRVTPKLTLDYGV
                     RFAWVKPPTLETGANFVPALYNPAEAPILYQPEVKTINGKKTTVAVDPTTGKTYPGAY
                     VGLFVPNTGNLSNGAITKQSKGYPAGLVYGTGIQVGPRFGFAYDPRGDGKTAIRGGFG
                     VFINPATQIGQEGDMTHNPPVEFVPQQYYGNVDDFLNAGTVIGPPNFGASFVLHPKEM
                     KVYGTSLGVQQDIGFGTVLSVGYVGNFARHLTGQRNINEVPYGAHLTQLYPSHANPAG
                     GVLPDNFFRPFPGYATITYRTTDLTSNYNSLQVQVTRRFKQGLEFGVAYTWSKAMDFT
                     DSYDGSVATYQDLRVWNYGPAGWDRRNNLVVNYLWSLPKGSRMWDNFATRAVLDNWQI
                     SGIAYYISGAPASFTLSTTDAADISAGGDGARVYLTGDPMRGAPHKFAEWFDTSVVHR
                     PSTATVSSSGTLVPSNGVSPFAPVVEPGYYNFDTALFKNFPIKEKFAVQLRLESYNTL
                     NHPEFNAVDEGAKFDPGGNQVNATFGQINGSNGPRVLQLAARINF"
     gene            complement(5184..6908)
                     /locus_tag="VFA02_03355"
     CDS             complement(5184..6908)
                     /locus_tag="VFA02_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HZU08914.1"
                     /translation="MKEHSPEDVLVPERLTRRQWLGRVPVPVIAATLGAGLMQERTAE
                     GQSNDAAADRGVRVYNIRNYGAKGNGITLDTTAVQLAINACFNDGGGTVLVPAGTFVI
                     GTVEIKSNVTLHISAGGKLLGSVDGKQYHAVEAIPLHGDSTLEDGNWALLFAVNAKNV
                     TIEGPGAIDGQGIQFHSPEHGVLPPSGLGGNRRPYHLLCYRCENLTVRNIDMLESAYH
                     SVRVIQSKRVHMDSIYIHNRVNGNNDGFHFISCEYVTVSNCIIQSQDDACALFGSCKY
                     ITITNSSFSTRWSVFRFGGGVAENITVSNCVLYQVYGCPIKFHGSPGSRFENMLFSNL
                     VLDDVTGPIHISIGPSNRTTTDDAAVEAKKGPAIARNISFSNIHGTVTTSPSQLPDFP
                     FHSGYRPGEGHSCITLNCVGDAVLENISFDDVHITFGGGGTAEEAARRELPQIAGEYF
                     MLGPMPAYGFYARNAKGITLQNVRFQVSAPDLRPAVILDHVEDAAISGLSVQGNTQAE
                     SVLRLIDSKQVLLTAARLLTPAAVFLQMEGRENEAIKIDGGDFSKASRLLAFKNGATE
                     GAIKVKDE"
     gene            7107..8294
                     /locus_tag="VFA02_03360"
     CDS             7107..8294
                     /locus_tag="VFA02_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZU08915.1"
                     /translation="MKILVQLSLVAVLTGTCFAQTTPATPVDLSKAVVDSTLARNPDP
                     KQFGGWGYAKALYLFGQYEVYMRTHEPRYLTFIKDWVDTHIDDNGNLDRQIDALDYVL
                     PANLVLILYQETHEEKYKLAAEKFRHRFDDYPRTTDGGFWHATVPSRQWQLWLDGIYM
                     SQPFLVHYGQIFGDKKYTEDEAVKQILIYHKHLQDPHGGLLYHAYDESGKQPWAAPVT
                     HHSSYFWCRAIGWYGMAIVDVLEVLPKDHPGRKELIKILDRLAKDLAKYQDPSTGLWY
                     QIVDKPNVSGNWPETSSSAMYTYILDVSVKRGYINKKYKKVAEKGHQGVLSKISIGTD
                     GLANLSDICEGTNVGDLNYYFNRKRNTNDFHGLGAFLLMNEEWNTSLTPTRVIENGLH
                     QSR"
     gene            complement(8277..9383)
                     /gene="dusB"
                     /locus_tag="VFA02_03365"
     CDS             complement(8277..9383)
                     /gene="dusB"
                     /locus_tag="VFA02_03365"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZU08916.1"
                     /translation="MKKHWDNPREHAMPEGVRVPAFVQIGNVTVAPATVLAPMAGVTD
                     TVFRRFIRNASVFSAEADFTAAVESEISNQQSGCGLIMTEFTSADGLTRMRESKRKRY
                     LTFYDDEHPISAQLFGSNPETLADAARIVEDTGFDMVDLNLGCPAKRVVSCNGGSGLL
                     RDLPLIAKIFEAVRAAVTIPFTVKFRLGWNDGHIICVELARLAQECGLQAVALHARTR
                     EQGYSGQARWEYIAAVKDAVRIPVIGNGDVRTPEDACALVAQTRCDAVMIGRAAPANP
                     WIFRQIAQYTATGRYDQPTDLDRYRMIRTYFQMLLEDGHPEAQGKMKQFASWFTHGVP
                     GGASLRKSIYESKTGAAILDAVERFFEPQLAALV"
     gene            complement(9439..9660)
                     /gene="rpmE"
                     /locus_tag="VFA02_03370"
     CDS             complement(9439..9660)
                     /gene="rpmE"
                     /locus_tag="VFA02_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZU08917.1"
                     /translation="MPKQGIHPNYHEIRVVCACGNTFTTRSTHKGDIHLEICSNCHPF
                     FTGKQKLVDTAGRVERFRRKYAKAETAAK"
     gene            10135..10222
                     /locus_tag="VFA02_03375"
     tRNA            10135..10222
                     /locus_tag="VFA02_03375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10169..10171,aa:Leu,seq:caa)
     gene            10323..11279
                     /locus_tag="VFA02_03380"
     CDS             10323..11279
                     /locus_tag="VFA02_03380"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HZU08918.1"
                     /translation="MPLDDRLGDLTLVQCTFTKAMPFAGTYFPLRTFYHDAMEIHPEP
                     LICGYPDQWSAFQLRHATFLQRLGHLKTALEVAFTRSYSSSSPEDRVIFTMGRLCAED
                     FWEIALLCANGYGFGALKLLRSLYELAVTMAYLCDNPSEVDAFLNYHWIAQHRLLEAL
                     KNSFGSGVLPEQVIEQTEREYAKHKEDYLVTICSKCDKKRVNHTWSKLDMVSMAKKTI
                     FGELIVPGYYEPMMHAHSTVNAIITRLEEINGSIGFNPDSQPKEADRALMTAHHVILG
                     VLDLQKKFFGLSVLNEPLQTCFQDFLDIWKEHRAKCQGLTVS"
     gene            complement(12418..>13090)
                     /locus_tag="VFA02_03385"
     CDS             complement(12418..>13090)
                     /locus_tag="VFA02_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZU08919.1"
                     /translation="HQRLQFEKEVLGFFVSGHPLDKYYEKLRNLPGVIDTATALEMKP
                     APSNGRRGQTPENDTAIAGIIAGLKVAKSKRSGELYAQASLEDITGKIDLICFPKDYE
                     RLKEVLKIEVPVLVRGQLRAEEDAAPKLCVSSIQALEDVKVKLPQNVRIRIPLERASD
                     ATLQELKQAIAASPGPGKLMLNLEQAGEFCVMLEPVGFSVAADRAFIERCEELLGRGM
                     VQAID"
BASE COUNT         3247 a         3580 c         3255 g         3008 t
ORIGIN      
        1 agaaggcgaa cgcaatccgg aacgtaaatc cggaacgcgg ctgcacgacc gcatcgtgaa
       61 cgcgccaaat tacccgcaga aactgagcgg cttcatgatt actgacgtca tgcaggcagt
      121 gagttatctg cggacacgcc cggatgtgga tccttcgcgc atcgcggtcc tcgggtattc
      181 gatgggttca tttgtgtccg cccttgccgg cgctgtggac ccccgcatca aagttcttgt
      241 cctctcgggc ggaggcgacc tcgatggccc gggaggttac tgggaaaaca gcggcaagcc
      301 catgtgccag tccgtcccct ggaagtccct ctccgttctg ggcgacaagg ccgccgtggt
      361 ctatgcctta aaccagcttc ggggcccgac ctttatcatg aacggaacaa aagataccgt
      421 cgtagacatc cccggcaaac atgagcaatc ctttttcgat gatctggcac accgcacaga
      481 ggccatcacc ggaacacgaa agaacatctt tgaaacgtat ttcataccgg acgcaagtca
      541 ccgccccaac tgggtaacgc ggcgcgccgc tctgtggctt caaagcaaac tgcactttcc
      601 gaattggact gcaaaaacca tcatggccat gccagagacc cacatcagcg aatgggccgc
      661 aaagaaccat gtagatatgg acagggctta tctttcagaa gacaaagaag gcggaatcat
      721 ggcgcttgga tctgatgttc cgaatgtgcc gcgcacacaa ctcaatgtgt ttactgatca
      781 ggaatttcag gaacaaaaat ccaggctggt ctatcaggcg tggacagcaa acgtactggc
      841 tgctgcgagc gaataaagat aggttccgaa acaggcttaa aattcaccgg agaacagggg
      901 aaatacaccc gcaatattgc cgtccaggca cagccatgcc ggaaatgaat gtaactatct
      961 ctgatgcaaa agagatttcg cgccttcacg atggcgagta gaaccatttc atagtctgag
     1021 ggattccgca ctgcctcgaa ctacccgggc cgccaacgga ctaacggaac tgatagtaat
     1081 attgcgcaca ggcctcctat gataaaaatg gccacgacct gctatatagc attttcacag
     1141 atagtctgaa ccatgccgcg aaactacagt cgccgcgacc aacagggagc cggcgacact
     1201 gcacaatgca aatggagtgt acagaatctg tgaaaatgcc gtaagcgccc ttcaatcgcg
     1261 gcataacgta cttctgcttc agtcctaatg ggagccatat gggtgatacc gtaatccggt
     1321 acgtttttct ttgagcaaaa ttcagcatac gtgctgacct ttcgcaccaa acgcgcagca
     1381 attgcaatca tttccagccc tgtatgccaa atctggaccc attcttcagt gaatcattat
     1441 ttttattcaa aaaaataatc gttgacactg ctggcttctg ctccctaact tgggtctccg
     1501 gcaaaggacc aattaaagat agtgtccttt tcctgtaaca acttcgaggc caggtaaaac
     1561 atgaaactct tcggaatgag caaaaaactt tgcaatcacc atctttgcaa aacacacttc
     1621 tgctggcttt ttgccgctgt tctgtttctg tgcagcggca cggaggcgat attgccccag
     1681 acatcaagcg gcacaatcag cggaagagtc gtagaccaga gtggcggtgc agtgacaacc
     1741 gcagatgtac ggctgatcaa tcaatggact ggcacgatgg tccgggcaac tgtacaacct
     1801 gatggcggat ttacctttcc cgacgtgcag ccgggcacat ttacagtaga cgttcgcgct
     1861 aatggatata aggagtacct gaagaaagac ctcgttctta gctcggcaga gcgcctctcc
     1921 gcaggcactc ttgtactgga gataggttct gtttctcaga acgtcaccgt cacggctgct
     1981 acgactcctg tccagacaca aagcgcggaa gtttccggcc ttcttgacac gcagcagctc
     2041 gataatctgc ttgccgtcgg gcgcgatttt atgagcatgg tgcgcacgat tcccggtgta
     2101 gtgggcggag gagccaatag tctgggaaca tctggaactc ctaccatcaa tggcctgcgc
     2161 aacgtcaata actcagggaa cattgatggc gtaaacggta gtccgcgcgg cggcgacaag
     2221 attgatactc cgccaaacct cgatgccatt caggaagtca aggtccttac ggcgaattat
     2281 caggccgaat atggagcggg gactgcgggg ccggtcatta atcttgttac caaaagcggc
     2341 acgcagcagt ttcatgggac tgcgtattac tacgtccgca atgaagcctt taatgcgaac
     2401 aactggttta acaattacaa caacctgccg cgtccgcaat accgctacaa cgatgttggc
     2461 ggcaacattg gcggccccat ctactggccc ggacacttca atacaaacag aaacaagctc
     2521 tttttcttct tctcgatgga atatctgccg aataagacgc cggatggatt gaagaaatac
     2581 atggtcccta cagcgctgga gagacaaggc gatttctcgc aaactcccgt ccagggcaaa
     2641 gtcagcccca atcccgccac cgactacatc aatatcaaaa agcccggcgc tgatccgggc
     2701 acctgccctg catcgggaac aggcggtaac catagtggct gctatccagg caatgtgctt
     2761 ccggcaaacg taatcaacag gcagatacag gcgttgctga atgttatgcc tctgcccaat
     2821 ttcaataacc gcgcagtcag cggaggcaac tacaactaca tcacgaatta cacaggaaac
     2881 aatcctgtaa accaggaaat ctttcgcatt gactacaatc cttccgacaa aatacatatg
     2941 ttcttccgcg gagagtttat gacggtgaat gatgattcct attcttcacc ggccaataaa
     3001 cttccctggc tgatgcgggt caattatcag acaacgcatc ctaatcttgc ttatgacatg
     3061 acctatgcat tcagcccaac gctgctgaat gaaattacag ttggaacttc aggatttggc
     3121 gagacgcagc tatacaacaa aagtgacctg ctcaaggcca ctaaaagcag caatggctac
     3181 aacattggcc agctctatcc cggcaataat cccatgaatc tgtttccggc tgtcagcttc
     3241 ggaggcattt ccaatgctgc gacctttgga tgggattcgc gcttccccat gtatgatcgc
     3301 acccggcaat acagtcttac agacagtctg acaaaggtcc tgggagcaca caatcttaag
     3361 ttcggcatca atctggcaac agaccattac ctgcaggcgc actcttcttc cggcataccg
     3421 gaaggctctt ttgccttcga ccgcaatgta aataatccga acgatagcaa ttacgctttt
     3481 gccaatgccc tgcaggggct gtttaacagt tacagcgaac ctaccgcccg ctccgattac
     3541 aatcctcgca tttacatcat cgaatggtac ggacaggacc agtggcgtgt aactcctaag
     3601 ttgaccctcg attatggtgt acgcttcgca tgggtgaagc cacccacact ggaaacaggc
     3661 gcgaattttg tccccgccct ttacaatccg gcggaagccc cgattcttta tcagccggaa
     3721 gtgaaaacta tcaatggcaa aaagacaacc gtggccgtgg atcctacaac tggaaaaact
     3781 tatcctggcg cttatgttgg attgttcgta cccaatacag gcaatctgtc caatggggcc
     3841 atcacgaagc aaagcaaagg ctatcctgca ggtcttgttt atggcaccgg tattcaggtg
     3901 ggaccgcgct ttgggtttgc ctatgatcct cgaggcgatg gcaagacagc aatccgcggc
     3961 ggcttcggcg tcttcatcaa tcctgccacg cagattgggc aggaaggcga catgacccat
     4021 aatccgccgg tggaattcgt accgcagcaa tattacggaa atgtagacga cttcctcaac
     4081 gcaggaacag taattggacc gccgaatttc ggcgcgtctt tcgtgctgca tccgaaagaa
     4141 atgaaagttt acggcacaag cctcggggtc cagcaggaca tagggtttgg cacggtcttg
     4201 agcgttggat atgtcggcaa ctttgcgcgt catctgaccg gacaacgcaa tatcaatgaa
     4261 gtcccctacg gcgctcatct cactcagctc tatccttccc atgccaatcc ggctggaggc
     4321 gtgctgcctg ataacttctt ccggcccttc ccgggatacg ccacaattac ctatcgcacc
     4381 actgacctga catcaaatta caactcactt caggtgcagg taactcgccg ctttaaacaa
     4441 gggctggagt tcggagtggc ttacacctgg tccaaagcaa tggatttcac cgacagctat
     4501 gacggcagcg tagcaacata tcaggacctc cgcgtctgga attatgggcc tgcaggatgg
     4561 gaccgcagaa ataacctggt tgtgaactat ctctggagct tacccaaggg gagccgcatg
     4621 tgggacaact ttgccacgcg ggccgttctg gacaactggc aaatctccgg aatcgcctat
     4681 tacatcagcg gggccccggc ttcgtttact ctctccacca cagacgccgc ggacatctct
     4741 gccggtggcg atggtgcgcg cgtttatctc acgggcgatc ccatgcgcgg cgctccgcat
     4801 aaatttgcgg aatggtttga cacaagcgtt gtgcaccgtc cctcaacagc cactgtaagc
     4861 tcatcgggca cattggtccc gtcgaatggt gtgtcgccct ttgctccggt cgttgagcct
     4921 ggatactaca acttcgatac agcgttgttt aaaaactttc ccatcaaaga gaagttcgca
     4981 gtacagcttc gtctggagtc atacaacacc ctgaaccatc ctgaatttaa tgcagtggat
     5041 gaaggcgcaa aattcgaccc cggcggcaat caggtcaacg caaccttcgg ccagatcaac
     5101 ggcagtaacg gcccgcgcgt cctgcaactg gcagcacgca tcaacttttg aacaggaggc
     5161 ggtctccagg ccgcctctac tcttcattca tcctttacct ttatggcgcc ttctgttgcc
     5221 ccattcttaa aggccagtaa tctggaggct ttggaaaagt cacctccatc tattttgatg
     5281 gcctcgtttt cccggccttc catctgaaga aatactgctg ccggagtcag aagacgcgct
     5341 gcggtcagca gcacctgttt tgaatcaata agacgcagaa cggattctgc ctgagtattt
     5401 ccctgcacgc tgaggccgct gatggcggcg tcttcaacgt gatcaagaat cacggcaggt
     5461 cgcaagtccg gcgcgcttac ctgaaaacgg acattctgaa gcgtgattcc ctttgcattg
     5521 cgcgcataga agccgtaagc cggcattgga ccaagcatga aatactcgcc tgcaatctgc
     5581 ggaagctcgc gccgggccgc ttcttctgcg gtgcccccgc caccgaaggt aatgtgaaca
     5641 tcgtcgaaag aaatgttttc aagcaccgca tcgcctacac aattcagagt gatacaggaa
     5701 tggccttcgc cggggcgata tccgctatga aatggaaaat ctggcaactg cgacgggctt
     5761 gtcgtgaccg ttccatgaat attgctgaat gagatattgc gcgcaattgc gggccctttt
     5821 ttggcttcta ctgctgcatc gtcggtagtt gtgcggttgg aggggccaat gctgatatgg
     5881 atgggcccag tcacgtcatc caggaccaga tttgagaaca acatgttttc aaagcgggac
     5941 ccgggagaac cgtgaaattt aatcggacat ccataaacct gataaagcac acagttcgat
     6001 acggtgatgt tctctgctac gcctcctcca aagcggaaaa cagaccagcg cgtgctgaaa
     6061 gacgaattgg taatcgtaat gtatttgcaa ctgccaaaga gggcgcaggc gtcgtcctgc
     6121 gattggatga tgcaattgct tacggtcaca tactcgcaac tgatgaagtg aaagccatcg
     6181 ttatttccgt tcacacggtt atgaatatag atactgtcca tatggacgcg cttactctga
     6241 attacacgga cgctgtgata agcgctctcc agcatgtcaa tgttgcggac cgtcaggttt
     6301 tcgcagcggt aacagagcag atggtatggt ctgcggtttc ctccaaggcc gctgggcgga
     6361 agaactccat gctcaggcga atgaaactga attccctgtc catctattgc gccagggcct
     6421 tctatcgtca cattctttgc attgacagca aacagcaatg cccagtttcc atcttccagc
     6481 gtcgaatcgc cgtgcagcgg aattgcttcc acggcatgat attgcttgcc gtccacgctg
     6541 ccaagtaatt ttccgcctgc agaaatgtga agcgtgacgt tgctcttgat ttcgaccgtc
     6601 cctatgacga aggtccctgc tggcaccagg actgtgcccc cgccatcgtt gaaacaggca
     6661 ttaatcgcca attggacggc tgttgtgtca agagtgattc cattgccttt ggcgccatag
     6721 ttgcgaatgt tgtacacgcg cacgccacgg tccgctgcgg catcattgga ttgcccttca
     6781 gcggtccgct cctgcatcag ccctgctcca agtgttgccg caatcacagg cacgggtacg
     6841 cggccaagcc actgtcggcg agtcaaccgc tcggggacga gcacgtcttc aggtgaatgc
     6901 tctttcaatg cactggccct cagttggatt tttcagcgta ggcgggcgaa agcagcgtgg
     6961 tcaatgccta tcttcgccat cgtcccgaat agcgggaaca tgggcgcatt gcaaacatct
     7021 ggacgtgtcc agtctcaaat agcggtactc ctgtggtgca agccgcaaaa ggaatgcgta
     7081 cagttgtacg tcatagagca gaaacgatga aaatccttgt acaactttct ctggtggctg
     7141 tgctcacggg cacctgcttt gctcagacca ctcctgcgac gcctgttgat ttgtccaaag
     7201 cagttgtgga ctccaccctt gcgcgcaatc cggatccaaa gcaattcggc ggttggggtt
     7261 acgccaaagc actctatctg tttggacaat atgaggtcta catgcggacg cacgagcctc
     7321 gctatcttac gttcatcaag gattgggttg acacacatat agatgacaat ggcaatcttg
     7381 accggcagat agatgcgctg gactatgttc tgcccgccaa tttagtgctc attctgtatc
     7441 aggagacaca cgaagaaaag tacaaactgg cagcagaaaa attccgccac cgcttcgatg
     7501 attatccgcg aacaacggac ggtggtttct ggcacgccac ggttccatcg cgccagtggc
     7561 agttatggct cgatggcatc tatatgtctc agccttttct ggtgcactat gggcagatat
     7621 tcggcgataa aaaatataca gaagatgaag ccgtgaaaca gatcctcatt tatcacaagc
     7681 atttgcagga cccacacgga ggcctgctct accacgcata cgatgagagc gggaagcagc
     7741 cctgggccgc tcccgttacg catcactcat cgtatttctg gtgccgcgcc ataggatggt
     7801 atggcatggc catcgtagat gttctggagg tattgccgaa ggaccatccg ggcagaaaag
     7861 aactgatcaa aatcctggac cgcctagcga aggacctggc gaaatatcag gacccatcaa
     7921 cagggctttg gtatcagatc gttgataagc caaacgtaag cggcaactgg cccgagacat
     7981 cgagttcagc aatgtatacc tacatcctcg atgtttcggt aaaacgcgga tacatcaaca
     8041 aaaaatataa gaaggttgcc gagaagggcc atcaaggcgt cctgagcaaa atttccattg
     8101 gcacggatgg tctcgcaaat ctctcagaca tatgcgaagg cacaaacgtc ggcgacttga
     8161 attattattt caatcgcaaa cgtaacacga acgactttca tggtctcgga gcattcctgc
     8221 tcatgaacga agaatggaac acgtcgctta caccaaccag agtcattgaa aacggcctac
     8281 accaaagccg ctaattgtgg ctcaaagaag cgttccaccg catccagaat cgccgcgcct
     8341 gtctttgatt cataaataga tttacgcaga gaagctccgc ccggaactcc atgcgtaaac
     8401 catgaagcga actgcttcat ctttccctgc gcttccggat ggccgtcctc gagcagcatc
     8461 tggaaatacg tgcggatcat ccggtagcgg tccaggtctg ttggctggtc atagcgtccc
     8521 gtggctgtgt actgcgcaat ctggcgaaaa atccaggggt tcgccggagc ggcgcgtcca
     8581 atcatgacgg catcgcaacg ggtctgcgca accagcgcac aggcatcttc cggtgtgcgc
     8641 acatcgccat tgccgatcac cgggatacgc accgcatctt ttactgcggc aatgtattcc
     8701 catcgcgcct gtcccgagta gccctgctcg cgcgtcctcg catggagcgc cactgcctgc
     8761 aggccgcact cctgagccaa acgcgccagc tccacgcaga tgatatgtcc atcgttccag
     8821 ccgagacgaa acttcaccgt aaacggaatc gtcactgccg cccgcaccgc ctcaaaaatc
     8881 tttgcaatca gcggaagatc gcgcagcagg ccgctgcctc cgttgcacga gacaacgcgc
     8941 ttcgcaggac aaccgaggtt caggtccacc atgtcaaagc cggtgtcctc aacaatgcgc
     9001 gcggcatccg ccagtgtctc cggattcgag ccgaacagct gcgccgagat cgggtgctcg
     9061 tcgtcataaa atgtcagata cctcttgcgc ttggattctc gcatccgcgt cagaccgtcc
     9121 gccgaggtga actccgtcat aataagcccg cagccggact gctgattgct tatctcagat
     9181 tccaccgcgg ctgtgaaatc cgcctctgct gagaaaacac tggcgttgcg gataaagcgg
     9241 cgaaagaccg tgtccgtcac cccggccatc ggcgcaagca ccgtcgcagg agcaaccgta
     9301 acattcccaa tctggacaaa cgcaggcaca cgcacgcctt ccggcatggc atgttcgcgc
     9361 ggattgtccc agtgcttctt catagcaaag cctccggcga gcggaggctt ctttcgtgca
     9421 acgcccttaa ccggttggtt acttcgcggc agtctccgcc tttgcatact tgcgacggaa
     9481 gcgctcaacg cggcctgcag tgtccaccag cttctgcttg cctgtaaaga acggatggca
     9541 attcgagcag atttccaggt gaatgtcgcc cttatgagtg gagcgggtcg taaatgtgtt
     9601 tccgcacgcg caaaccactc gaatttcgtg atagttagga tgaatacctt gtttcggcat
     9661 gattcaaaaa acctttccct gcaatctctt tattttacgg agattatgcc tatttcgcaa
     9721 cgatgacgat aggggtgttc gtagcaagtg atatgtccgg tttttcttag cagttgaaat
     9781 gtcctgttgc tttatagcat ttcatatgta gagtccgtca tcctgacgac cgatcgatat
     9841 aagtccgtca ttctgacgtc cgcagggagg aagattctta acgatgtagg gtttagttag
     9901 ccatccttct aagattttcc gcgctcccac gaatgatggc cggtgaaaaa actcaaatca
     9961 taaggcactt cagccctggc cttcgcttca agactatatg agaccgcttc tggtagatcc
    10021 aaacgaaacc catatgcacc tgctatgtct gcggaattcc gccctgctcg cttgaaaatc
    10081 catgcttatg tcatactgaa caagacgttc cggaaaatcc caccactcct acaagccggg
    10141 atggcggaac tggcagacgc agcggactca aaatccgccg gcccttgcgg ccatgggggt
    10201 tcgacccccc ctcccggcac cagcaaaact aagatttaga ttgattttgg cccctcttga
    10261 ggccaaaatc attggtggct gttggctgcg gcggtggcat acacctgatt ttcctgtgct
    10321 gaatgccgct ggacgatcga cttggcgatt tgacactcgt ccagtgcacc ttcactaaag
    10381 cgatgccatt tgcggggacc tatttcccgc tcaggacttt ttatcatgac gctatggaga
    10441 tacaccccga accgctgatc tgcggatacc cggatcaatg gagtgcgttt cagctgcgcc
    10501 acgcgacatt tctgcaacgc ttgggacatt tgaagaccgc gttggaggtc gcattcactc
    10561 gttcgtatag ctcaagcagt cccgaggatc gcgtgatctt cacgatggga agactgtgcg
    10621 ccgaggactt ttgggagatt gctttgctct gcgctaatgg ttatgggttt ggcgccctga
    10681 agctattgcg ctcgctctat gaactggccg tcacgatggc gtatctgtgc gataaccctt
    10741 cggaagtcga cgccttcctg aattatcact ggatcgccca acaccgactg ttggaggcgc
    10801 tgaagaacag tttcggttct ggcgtcctgc cagaacaggt catcgagcaa actgaacgag
    10861 aatacgcgaa gcacaaggag gactatctcg taactatctg ctcaaaatgc gacaagaagc
    10921 gggtaaacca cacgtggagc aaattggata tggtgtcaat ggctaaaaaa acgattttcg
    10981 gcgagctcat cgtgcctggt tattacgagc cgatgatgca cgcccacagt acggtcaacg
    11041 ccatcattac ccgcctcgaa gagataaacg gaagtatcgg ctttaatcct gattcgcagc
    11101 ccaaggaagc tgatcgtgcg ttgatgacgg ctcaccatgt cattctcgga gttctggatt
    11161 tgcagaagaa attcttcggg ctgagcgtgc tgaacgaacc gctccagacg tgctttcagg
    11221 attttctcga tatctggaag gagcataggg cgaaatgcca gggattaact gtgtcctgag
    11281 acgtttctct ggacggcccc gcggcatgct tcgtagaacg gctaactaaa gacatggaaa
    11341 aacttaggta ctctgaaatt ctcggccgct taaagggctt tgattcctga ctcaccagtc
    11401 tgaaaattac accccgtccg aacgatagaa tccatcaagc atttgaagta ctccgtagag
    11461 ctgacagggc gagtcgcacg aattgcgctg ggttaacggc caccgtgccg ttgcagacac
    11521 cctcccgatg aatgtcgttt ccaccgggaa aatccctgtc aagtctgccc tgtccttttt
    11581 ggacataact cacacaaaac aaaggtctga tttcgcaaaa tagatgtcca tttctttcgg
    11641 cccggtttgt tatcctgaat atagaggcaa atcttccaga aaactcccgc aagccatgaa
    11701 catcagccct ttgggtttga cattggcttt tgagcgaggg acaaacttct tagtttgcaa
    11761 tcggaacgcc cgcaaagccg cggacgtctt cttctgcact gaaccctgag atgagagacg
    11821 gcataagtag acaatccaga gatggaccgc tgcaaaaata cacgcaaatg ttttgttttc
    11881 aggaatttaa taaaaattaa gcctcattta tctacccata gatcatctat gaaaatttct
    11941 ccggctattg catttcacag atagcctgga gcctgcacga aactacagcc gccgcgacca
    12001 acaacgagtc ggcgaagccg tacagtgcaa atggagtgta cagaatctgt gaaaaatgcc
    12061 gtagagcatt tgttttcagg aatttaactt aaattacact cgtttctatg tatccataga
    12121 ttatctatgg gggctatttt tggatcttgc ttttcacata atccgaacca tgccacgtaa
    12181 gttattgaaa acaaacggtt cccgtacaga cagccacctg taacttattg aaaacaaagg
    12241 attcccgtac aaaagcccgg gggggggtag ggggttattc aagccgaagc ttgagtgaga
    12301 gcagcttcca gaaaggaatc cgggtgtcca aactaaccgc acggcttata aaaccaattc
    12361 ttgcaggaat ttacaaaaag taaaaacacc cggggcatac ctcgaagaaa tcgctcatca
    12421 atcaatcgcc tgcaccatgc ccctgccgag cagttcctcg catcgctcaa taaacgcgcg
    12481 gtctgcggcc acactgaacc ccacaggctc cagcattacg cagaattctc ccgcctgctc
    12541 caggttcagc atcagttttc ccggacctgg agacgcagca atcgcttgct ttaactcctg
    12601 cagagtggcg tcactggccc gctccagtgg aatgcgaatg cgaacattct gcggcagctt
    12661 taccttgaca tcttcgagtg cctggatgga tgaaacacac aacttagggg cagcatcttc
    12721 ttcggcgcga agctgcccgc ggacaagcac cggcacttcg atcttcagga cttccttcag
    12781 gcgctcgtag tctttcggga agcagatgag gtcaatcttt cccgttatgt cttcgagcga
    12841 agcctgggca tagagttcgc cggaacgctt ggatttggcg accttcagcc ctgcaatgat
    12901 tcccgcaatg gctgtgtcgt tttccggggt ctgcccacgg cggccattgc tgggggcagg
    12961 tttcatctca agcgccgttg ctgtgtcaat cacgcccggc aaattgcgca gtttctcgta
    13021 atatttgtcc agcggatgtc cggagacaaa gaacccaagc acctctttct caaactggag
    13081 acgctggtgc
//
LOCUS       DASRBV010000082        13800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_612972, whole genome shotgun sequence.
ACCESSION   DASRBV010000082 DASRBV010000000
VERSION     DASRBV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612972"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFA02_03390"
     CDS             complement(<1..198)
                     /locus_tag="VFA02_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08920.1"
                     /translation="MSLDLRIPMGLMFTIVGLILSLYGLFTRSSAIYERSAGMNINLI
                     WGIVMLVFGVTMFLLGRASDRR"
     gene            complement(223..2040)
                     /locus_tag="VFA02_03395"
     CDS             complement(223..2040)
                     /locus_tag="VFA02_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU08921.1"
                     /translation="MDLTLIDWLIMLLYFAFVLGIGFALKRFMRTSKDFFQAGRSLPA
                     WICGLAFISANLGAQEVIGMGASGAKYGIATSHFYWIGAIPAMIFVGVFMMPFYYGSK
                     ARSVPEFLRLRFDEKTRAYNACSFAVMTVFSSGISMYAMARLIQTLHVFDSLFIRLNM
                     PVEWIFHFAIFVSAIIVLGYIFAGGLTSAIYNEVLQFFLIVAGFLPLVWVGLKNIGGW
                     SGLKAALPAAYTHSWHGMTNPNHNPLGVEWFGLAMGLGFVLSFGYWCTDFLVIQTAMA
                     SDSEESARRVPLIAAIPKMFFPFLVILPGLIAIATPTVTQHAMLQTAPAATASVSTDV
                     SSTHYSKGLIPAKINPVTGKVMTDEHGNPVLDYDLAIPNMLLHYFPTGILGLGLTALL
                     ASFMSGMAGNVTAFNTVWTYDLYQSYIKKDAPDTHYLKMGRWTTVVGILISIGTAYIA
                     TSFNNIMDTLQLVFSFVNAPLFATFLLGMFWKRTTGHGAFTGLVSGTVAAVLHHGLTL
                     PVTAHPGIHGGWIAILHTYPSEMAQNFWTAIWAFSTNFVLTVIVSLMTKPRPEGELVG
                     LVYSLTPKPVEHHMAWYARPKVIAVGLLVLLVALNMIFR"
     gene            complement(2252..2695)
                     /locus_tag="VFA02_03400"
     CDS             complement(2252..2695)
                     /locus_tag="VFA02_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08922.1"
                     /translation="MKRFCNKVIAMMFALFLGAACAQAQYGPPPPPPPPRPAHPPMIF
                     LGMAHVDGLTDHDDIKVGPYAGRYHSIVLRVRYAPIQFEQVVIHYGNGTAQTLPVKAF
                     VRAGGQSNPVVLPGGDRVIASLELWYSRANPNNPNRPEVRLFGMP"
     gene            complement(2958..4958)
                     /locus_tag="VFA02_03405"
     CDS             complement(2958..4958)
                     /locus_tag="VFA02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZU08923.1"
                     /translation="MSAPFHSQLTAAPAKDEFAWVSDQQGHRNVWIAVKRGDGFSASQ
                     ITKYTEDDGQEISGVTFTPDAESVVYVRGGDSEFPEKPAPNPALISEGVEQQIWIVPA
                     AGGAPRKLADGAAPAVSPAGDSVAYILKGQIWGVPLRDMNAKPEQWLHTRGRASSLLW
                     SPDGKYLAFVSHRGDHDFIGVYVRETKTLHYLDPGTDHDGHPVWSPDSKQVAFIRVPY
                     NKKEEIFAPVRTGYPWSIRVADVETGKGREIWRAQEGMGSVFQGTESEHQLFWSAHTI
                     VFPWERDGWLHLYAVPADGDEAKLLTPGEFEVDAVTSSPDKKSIVYSSNQDDIDRRHI
                     WKVDVASGKVTQLTRGEGIETSPAVASDNQTIVVLRSDARVPIRPAVVEANGVLRDLA
                     PQSIPADFPAKSFVVPQQVIFSAADGLRIHGQLFLPSNAGDGKRHPAVVFFHGGSRRQ
                     MLLGWHYMQYYSNAYAMNQYLASLGYVVLSVNYRSGIGYGEQFREALNYGATGASEFN
                     DVLGAGLYLRGRPDVDPAHIGVWGGSYGGYLTALALARASDLFAAGVDLHGVHDWNLE
                     LGNWLPAYDPAADPEAARIAWNSSPMASLSTWRSPVLLIQGDDDRNVQFSQTVQLAAA
                     LRRQKVDVEELVFPDEIHDFLLHRDWVAAYSAGAEFFGKHLK"
     gene            5298..6245
                     /locus_tag="VFA02_03410"
     CDS             5298..6245
                     /locus_tag="VFA02_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZU08924.1"
                     /translation="MRILVTGAAGFLGSHLTDALLSEGHSVLGVDNLCTGSLENIRHL
                     KNEPRFEFQKHDICLPFDFGKIDYIFNFASPASPQDYSRLGIETLAVGSDGTRNTLEL
                     AEKYRAGFLHASTSECYGDPAVHPQTEEYWGNVNPIGPRSVYDEAKRFSEALVMAYHR
                     YRKVDTRLVRIFNTYGPRLQANDGRVISNFLVQALQGQDLTVYGDGSQTRSFCYVSDE
                     IDGILRLSRSDMHLPVNIGNPHEWTILECAKTVLRVTGATSKIVFEPLPVDDPMQRKP
                     DISRARKLLGWEPKVDLETGLRLSLEYFRETLPLRAGPA"
     gene            6347..6730
                     /locus_tag="VFA02_03415"
     CDS             6347..6730
                     /locus_tag="VFA02_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU08925.1"
                     /translation="MCTTILLLEDDPVQAATRRAILASAGNTVVVVKDPREALSILDD
                     PNSQIGLVITDHFMPGMNGPQFVSELRKKDPSMPVLVLSGMPGVEPEYNGLDVMHRVK
                     PFSPTDLILLTQTILGSRQRLSRTA"
     gene            complement(7122..9818)
                     /locus_tag="VFA02_03420"
     CDS             complement(7122..9818)
                     /locus_tag="VFA02_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU08926.1"
                     /translation="MKATKLCLSATVALMSALSGFCQSNAQKPLYLDPSQPAERRAAD
                     LVSRMTLEEKVSQMQNHAVAIPRLGVPEYDWWSEALHGVARAGYATVFPQAIGLAATW
                     DTDLEHRIASVISTEARAKFNEAQAEGDHSIYRGLDFWSPNINIFRDPRWGRGQETYG
                     EDPFLTGQMGAAFIRGMQGDDPRIFKTIATAKHYAVHSGPESTRHEANIDPLPHDVED
                     TYLPAFRMAMVDAKAGSIMCAYNSVNGEPACANKFLLDETLRKKWGFQGYVVSDCGAI
                     SDFFSANGHHFSPDAAHASAAAVKAGDDLSCGTEYAALVDAVHQHLIDEKAIDQAVER
                     LFTARFKLGLFDPPGSDPYSRISPGENATPEHAELALKAAQESIVLLKNEHNTLPLSN
                     AVKTIAVVGPNAESLITLEGNYNGAPLHPVTPLEGIEQKFKSAKVLYSQGAPLVAELP
                     VVVPSNVFRDKDGQPGALTAEYFSNADFSGTPVTQRDAQIDFDWNRVAPVPVRSAKQF
                     SVRWSGSFQPLAPGDVTFTVQPARCEPYCAAPYAYSVSVDGKQVASVTNDNPRHNNRP
                     LSFTVHFGDAKPHPFEMKYSRKGTDYGAGITLKWKPDETQLRQQAVEAAKQADVVVAF
                     VGLSSEVEGEEMPVHIPGFSGGDRTDIALPAAQRALLEAVAATSKPLIVVLLNGSALA
                     VDWAQEHAAAIVEAWYPGQAGGTAIADVLSGDANPAGRLPVTFYKSVDQLPPFDDYKM
                     EGRTYRYFRQKPLYGFGYGLSYSSFTYKNLRFSWQPMNAGALLHVTADVENTSSVPGD
                     EVAELYITPPAAPGAPNHWLAGFRRVHIGPHETAHVAFDLDARNLSMVDAKGERAMAA
                     GTYKVFIGAAQPGETSSGVEQKLTIAGPQNVSEDALGASTAK"
     gene            9920..10663
                     /locus_tag="VFA02_03425"
     CDS             9920..10663
                     /locus_tag="VFA02_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZU08927.1"
                     /translation="MRISLFITCYNDTLFPETGKAVVQLLERLGHEVEFALEQTCCGQ
                     MHYNTGYQQEAMPLMRRFVEIFGDAEVVCIPSSSCVAMIRDHYPKMAEATGNAALIKA
                     VEDLLPRVYELSELLVNKLGLEDVGAYYPHRVTYHASCHSLRSLHLGDLPLRLLRNVR
                     GIDLVELQDIDQCCGFGGTFAVKMGDVSAAMLADKIRSVEDTGAEVCTAVDNSCLMHI
                     FGGLHRLRAGVKTVHLAEILASTEEGMAR"
     gene            10660..12084
                     /locus_tag="VFA02_03430"
     CDS             10660..12084
                     /locus_tag="VFA02_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HZU08928.1"
                     /translation="MSIRVGQASEAPPFQKAALPLLDDTQLRKNVRHATNVITRKRAV
                     VVGEQQDWQQLRASASAIKEHTLRHLDHYLEQFEKNCTAAGGHVHWAADADEANRIIV
                     DLIRQHGGKEIIKVKTMTSDEIGLNRALEANGIHPHETDLADMIIQLGHDKPSHIVVP
                     ALHKNRQQVREIFMQAMGLKELGDRPEDLTTAARNYLREKFLKVKIGFSGANYLIAET
                     GGVCVVESEGNGRMCLTLPDTLITIAGIEKVIPTFRDLEVFLQLLPRSATGERMNPYN
                     SLWTGVHKGEGPQEFHVILLDNGRTRILSDHESRQTLRCIRCGACQNACPVYRQTSGL
                     AYGSIYAGPIGAILTPQLFDMQHAQTLPYASSLCGACYEVCPVKINIPEVLIHLRGRV
                     VREQQSSANLEALGMKAMSRIFLSRRSFEAAQRLGRIGQWPFVSDGWVRHLPGMLGNW
                     TAVRDLRPMPKQTFRSWWQERKKT"
     gene            12147..12776
                     /locus_tag="VFA02_03435"
     CDS             12147..12776
                     /locus_tag="VFA02_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZU08929.1"
                     /translation="MPETNAREVILDRIRHANADAAPSEYSVIERTYRQKAGMDRAEI
                     QKAFAERLHEYDAHVTEATAETLSAAIAESLRRNNQKSAIVASGFPQQLLPEGFLWQQ
                     ESSATTEQLNTAEGAICACEVAIAHTGTIVIRNARRLTLLPDRLLCVVYEDQVVETVP
                     EAFARLQPFASEPLTFISGPSATADIEMTRIRGVHGPRFLDVVLVRNSQ"
     gene            12860..>13800
                     /gene="ispG"
                     /locus_tag="VFA02_03440"
                     /gene_synonym="gcpE"
     CDS             12860..>13800
                     /gene="ispG"
                     /locus_tag="VFA02_03440"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZU08930.1"
                     /translation="MPEIQRRKTVTVRIGNVRVGSEAPVVVQSMTNTDTSDVQATIQQ
                     VAALAAAGSELVRVTVNNDDAAKAIPYIVEGLAKMGIHVPIIGDFHYNGHILLKKYPD
                     CAKALAKYRINPGNVSIGRKDDENFRTMIECAVENNKPVRIGVNWGSLDQALLTRLMD
                     ENSKRPDPLDARDVMMEAMCRSALDSAAAAERYGLGHDKIIISGKVSGVRDLIDVYQM
                     LAARCDYPLHLGLTEAGMGMKGIVASTAGLAPLLLKGIGDTIRVSLTPKPGGDRTEEV
                     LTAQQILQSLGIRSFTPQVTACPGCGRTTSTFFQELA"
BASE COUNT         3151 a         4038 c         3692 g         2919 t
ORIGIN      
        1 ccggcggtcg ctggcgcgtc ccagcaggaa catagtcact ccaaaaacca gcatcacgat
       61 accccagatg agattgatat tcatcccggc tgagcgctca tagatggcgc tgctgcgcgt
      121 aaacagccca tacagcgaaa gaatcaggcc gacaatcgta aacatcaggc ccatgggaat
      181 gcgcaaatca agactcattg cttttctcca agctgcattt ctctaccgga agatcatatt
      241 caacgctacc agcagcacca gcaatccaac ggcaattact tttggacgtg cataccaggc
      301 catgtgatgc tcgactggct ttggagtcag cgaatatacc aacccaacca gctcgccctc
      361 aggacgcggc tttgtcatca ggctcacaat caccgtcaga acaaagttgg tcgaaaacgc
      421 ccagatggcg gtccagaagt tctgcgccat ttcgctggga taggtatgca gaatggcaat
      481 ccatccaccg tgaatgccag gatgcgccgt caccggaagc gtcagcccgt ggtgcagcac
      541 agcggcaact gttccactta ccagtcctgt aaacgcgcca tgcccggtcg tgcgcttcca
      601 gaacatgccc agcaggaagg tcgcaaacag cggagcgttc acgaacgaaa agacgagctg
      661 gagcgtgtcc atgatgttgt tgaacgaggt ggcaatatac gctgtcccga tcgaaatcag
      721 gatgcccacc actgtcgtcc atcggcccat cttcaggtag tgcgtgtctg gagcgtcttt
      781 tttgatatag gactggtaca ggtcataggt ccagacagta ttgaaggctg tcacgtttcc
      841 tgccatgccg gacatgaagc tcgccagcag cgcggtcagc cccaggccca gaatgcccgt
      901 cgggaaatag tgcagcagca tgttcggaat ggcaaggtcg tagtcaagca ccggatttcc
      961 gtgctcatcg gtcatcacct tgcctgtgac gggattgatc ttggcgggaa tcagtccctt
     1021 gctgtagtgt gtgcttgaaa catcagtgct cacgctggcc gtagccgccg gcgctgtttg
     1081 cagcatggcg tgctgcgtga ctgtgggtgt agcgatggca atcagtcccg gcaggatgac
     1141 cagaaatggg aaaaacatct tcggaatcgc cgcaataagc ggaacgcgcc gcgccgattc
     1201 ttctgagtcg gacgccatcg ctgtctgaat cacgaggaaa tccgtacacc agtagccgaa
     1261 cgaaagcaca aaccccaggc ccatggccaa accaaaccat tccacgccca gcgggttgtg
     1321 attgggattg gtcatgccat gccacgaatg cgtgtaggcc gcagggagtg ccgctttcag
     1381 accgctccac ccaccaatat tcttaagccc tacccagacc aacggcagga acccggcaac
     1441 aatcagaaag aattgcagca cttcgttgta gatggcgctc gtcagaccgc cggcaaagat
     1501 gtacccgagc acaatgatgg ccgagacgaa aatcgcgaaa tggaagatcc actccacagg
     1561 catgtttagc ctgatgaaga gcgaatcgaa gacgtgcagc gtctgaatca gccgcgccat
     1621 ggcatacatc gaaatgcctg aagagaagac cgtcattaca gcaaaagagc aggcgttata
     1681 ggcgcgcgtc ttttcatcga agcgcagccg caaaaattca ggcacggagc gggcctttga
     1741 gccgtaatag aacggcatca taaagacgcc cacgaagatc atcgccggaa tcgcgcctat
     1801 ccagtagaag tggcttgtgg caatgccata ctttgcgccg gaagcgccca tcccgatcac
     1861 ttcctgcgcg ccaagattgg ctgagatgaa ggcgagtccg caaatccagg caggcagtga
     1921 gcggccggcc tggaagaagt ccttgctcgt ccgcataaac cgcttaagcg caaagccgat
     1981 gcccagcaca aaagcgaaat acagcagcat gatgagccag tctatgagtg tcaggtccac
     2041 gtgcccggtt cttcccttct atttcagata aaggtattga gggtaatgca cgcagccgtg
     2101 cccgcagcac caaggcaaat ctgagcagcg gctacggagg ctgttctcac tgcaatgtac
     2161 acgctttcat aagaggtcgt ccagagggaa aacttcgcgt ccggggccac ccgctccagg
     2221 ttttctgatt tgagcgggta gccgcatagc tttacggcat cccaaacagc cgcacctctg
     2281 gcctatttgg attgttcggg ttcgcgcgcg aataccacag ctccaggctg gcaatcacgc
     2341 ggtcgccgcc aggcaggaca accgggttgc tctgccctcc ggcgcggaca aaagccttca
     2401 ccggaagcgt ctgcgccgtg ccattcccgt agtggatgac aacctgctca aactggatcg
     2461 gagcatatct tacgcgcagc acaatcgagt gatagcgccc tgcatagggg ccaactttaa
     2521 tgtcatcgtg gtcggtcaga ccatccacat gagccattcc caggaaaatc ataggcggat
     2581 gcgcgggacg cggaggcggc gggggcggag gtggaccgta ctgcgcctgt gcgcacgcag
     2641 cacccaaaaa cagtgcaaac atcatcgcaa taactttgtt gcagaatctt ttcatgctct
     2701 ttcctttcac gttcccaaca gacacggaac cgcatgcaga agttgcgggc tgaaagtgct
     2761 gaaaacaaga tgcgacagga attctgctgg aacgggtgtc ccacatcttg aaaaaatgtg
     2821 ggacggtagc cttcacccat acgcaagaaa cgacgcagct ttataaagcc ggttccacaa
     2881 gcctgattgg gcagcccccc ggagttcaga ccgttgaatc agggccatcg gctcacgtag
     2941 ggatgatcct ttccctctca cttcaaatgc ttcccaaaga actccgcgcc agccgaatat
     3001 gctgcaaccc agtctctgtg cagtagaaaa tcgtgtatct catccgggaa aacaagttct
     3061 tccacatcta ctttctgcct gcgcagagca gccgccaact gcacggtctg tgaaaactgg
     3121 acgttgcggt catcatcacc ctgaatcagc agtacaggcg agcgccatgt gctcagcgaa
     3181 gccatcggcg aagaattcca cgctatgcgc gccgcctccg ggtccgccgc cggatcatac
     3241 gccggaagcc agttgcccag ctccaggttc cagtcatgga caccgtgcag gtctacacct
     3301 gcggcaaaca ggtcggaagc gcgtgccaga gccagtgccg tcaggtagcc gccgtagctt
     3361 ccgccccaca cgccgatgtg cgctgggtca acatctggac gcccgcgcag atacaatcct
     3421 gcgcccagaa cgtcattgaa ttcgcttgct ccggttgcac catagttcag agcttcgcgg
     3481 aactgttcgc catagccgat gccgctgcga taattcactg acaacaccac ataacccagg
     3541 ctggcgagat actggttcat cgcatacgca ttcgagtagt actgcatgta gtgccagccc
     3601 agaagcatct ggcggcgcga tcctccatga aagaagacta ccgccgggtg acgcttccca
     3661 tcgcctgcat ttgaaggaag aaaaagctgc ccatgaatgc ggagtccatc cgccgcagaa
     3721 aaaatcacct gctgcgggac tacgaagctt tttgcgggaa aatcagccgg aatagattgc
     3781 ggcgcaaggt cgcgcaacac tccattggct tccaccactg ccggacgaat tgggacacgc
     3841 gcatccgagc gcagcacaac aatggtctgg ttatcactgg ccactgccgg agaagtctca
     3901 attccctctc ctcgcgtcaa ctgcgtgacc tttccggaag ccacatccac cttccagata
     3961 tgcctgcggt ctatatcgtc ctggttcgag gagtaaacaa tgctcttttt gtctgggctg
     4021 ctggtgacgg catctacttc aaattcaccc ggagtcagca attttgcttc gtctccgtcc
     4081 gcgggaacgg catacagatg cagccatccg tcgcgctccc acggaaagac aatcgtgtgt
     4141 gcggaccaga aaagctggtg ctcgctttct gtcccctgga agacgctgcc cattccttcc
     4201 tgcgcgcgcc aaatctcgcg gcctttgccc gtctccacat cggccacgcg gatagaccat
     4261 gggtaacctg tgcgcacagg cgcaaaaatt tcttctttct tgttgtacgg aacgcgaatg
     4321 aacgcaacct gcttactgtc cggcgaccac accggatgtc catcatggtc tgtcccgggg
     4381 tcgagatagt gcagagtctt cgtctcgcgc acatacactc caataaaatc gtggtctccg
     4441 cgatggctca caaaggcaag atatttcccg tccggcgacc acagcagcga cgacgccctt
     4501 ccccgcgtat gcaaccattg ttccggcttt gcattcatat ccctcagcgg gacgccccat
     4561 atctgccctt tcaggatgta tgccacactg tctcctgccg gtgaaacggc aggcgcggcc
     4621 ccatcggcca gcttgcgcgg agcgccaccc gccgcaggca caatccatat ctgctgctcc
     4681 acgccttcgg aaattaatgc cggattcggc gcaggctttt ccggaaattc cgagtcgcct
     4741 ccgcgaacat aaaccacgga ttcggcgtcc ggagtgaatg tgactccgct gatctcctgt
     4801 ccgtcgtctt cggtgtattt ggttatctgg cttgcagaaa atccatcgcc ccgcttaacg
     4861 gcaatccaga cattgcggtg tccctgctga tcgctgaccc aggcaaattc atcttttgca
     4921 ggcgccgccg ttaattgcga atggaaaggc gcgctcatgg cctgttggag ggtgaaactc
     4981 tgcgcgaaaa caccgggtgc gactgtccag aaaaacaata tgagacatct gcgaacagca
     5041 ttcatgcggc ttctcctgaa agccgcatca tacaggaacg catatgaaaa tcagagggcg
     5101 ggagcaacat ttcattccat ccctgctatc ttagagatag ctcatttact tcaatcatga
     5161 gacactgatt tcgtctgggc ttgaagcgcc gcaagcatag atcgcttacc cggcctgatc
     5221 agagattttc ctgtatgcca tcgcggtccg taagtttaca ttaacttctt catatctcat
     5281 ttaaggcact tataacgatg cgaattcttg ttactggcgc agccggcttt cttggatctc
     5341 accttactga tgctctcctc agcgaaggcc attctgttct cggcgtggac aatctatgca
     5401 ccggctcgct tgagaatata agacatctga agaatgagcc gcgctttgag tttcagaaac
     5461 acgacatctg cctgcccttt gactttggca agatagatta catcttcaac tttgcttcgc
     5521 cagccagtcc gcaggactac agccgcctgg gaatcgaaac gctggccgta ggctcagatg
     5581 gcacgcgcaa tactcttgaa ctggcggaga aatatcgggc agggtttctc catgcgtcca
     5641 cttcagagtg ctacggcgat ccggcggtcc atccgcagac ggaagaatat tggggcaatg
     5701 taaaccctat tggaccgcgt tccgtatatg acgaagccaa gcggttttct gaagcgcttg
     5761 tcatggccta tcatcgttat cgcaaagtgg acacgcgcct ggtccgcatc ttcaatactt
     5821 atggtccgcg gcttcaggcc aatgatggcc gagtgatctc caatttcctg gtacaggcat
     5881 tgcagggcca ggacctgacg gtctatggcg atggctcgca gacgcgcagc ttctgctatg
     5941 tctcggacga gattgacggc atcctgcggc tctcgcgctc tgacatgcat ttacccgtca
     6001 atatcggcaa cccacatgaa tggaccatcc ttgagtgcgc aaagaccgtc cttcgtgtga
     6061 cgggagcaac aagcaagatc gtctttgagc cacttccggt ggatgatcca atgcagcgta
     6121 aacctgatat cagccgcgcc agaaagcttc tgggatggga accgaaggtt gatctggaaa
     6181 ccggcctgag actgagcctg gaatatttca gagagacgct gcccttgcgc gcaggcccag
     6241 cctgaaataa tctcgttttt ttccagagca ttcaggaaaa ccctattggg gagtcctgca
     6301 tctcaggtta gagttgtatt tctgttgggg gctgcaaccg ataacaatgt gtacaacaat
     6361 cttgcttttg gaggatgatc ccgttcaggc cgccaccaga agagccattc tcgcaagtgc
     6421 tggcaatacc gttgttgttg taaaagaccc tcgcgaagcc ctctctattc tggatgaccc
     6481 aaattcccaa attggcctgg taattactga ccacttcatg ccgggaatga acggcccgca
     6541 gtttgtctcg gaactgcgca aaaaagaccc ttccatgcct gttctcgttc taagcggtat
     6601 gcctggagtc gaaccggaat acaacggtct tgatgtgatg caccgggtaa aacccttttc
     6661 acctacggat ttgatcctgc ttacgcaaac gattcttgga tcgcgacaac gtttaagccg
     6721 cactgcctga tagaattcag aaaaataccc atatcagtac aaagaaatac tgttttttct
     6781 tgaccatttc ccgccccagg tctaacacta gctttttaag aattttggac ccgcccgaaa
     6841 gtcctgttgt ttcaggattt tgggtaagcc tgtaagtggt tttcttgttt cggaacttaa
     6901 catatccatg ggcaaaacca aacagatgtg tcccaagtgc aagagtatga cgatggtccg
     6961 cgtccatcgc cacggcttct tacaacagca ggtcttcccc aaactgggtc tctatccctg
     7021 ggaatgcctt acctgccgcg aggtcttcct taaaaagagc cgcgggcacg ggtataaaaa
     7081 agacctacag aatacctgac gcgccccgca accaatttcg gttatttcgc agttgaggcc
     7141 cccaaggcgt cttcactgac gttttgcggg ccagcaatcg tcagcttttg ctccacaccg
     7201 gaagacgtct cgccgggttg tgccgcgcca atgaacactt tgtaagtgcc cgcggccatg
     7261 gcccgctcgc cctttgcatc taccatgctc agattgcgcg cgtcgagatc aaatgcgacg
     7321 tgtgctgttt cgtgcggccc gatatgcacg cgcctgaatc cggcgagcca atgattcggc
     7381 gcaccgggag cagcaggcgg agtgatgtac agctcggcca cctcatctcc aggaacggaa
     7441 cttgtgttct ctacatctgc tgtcacatgc agcagagcgc ctgcattcat gggctgccac
     7501 gagaagcgca gatttttgta agtgaagctg gaataactca gtccgtagcc gaatccatac
     7561 agtggcttct ggcgaaagta gcggtaggtg cggccttcca ttttgtagtc gtcaaaggga
     7621 ggcagctggt caacggactt gtaaaaggtc accggaagcc ttcctgccgg attggcatcg
     7681 ccgctaagaa catcagcaat cgccgtgcct ccggcctggc ctggatacca cgcctccaca
     7741 attgcagctg catgttcctg tgcccagtct acggccagcg cgctaccgtt gagcagaacg
     7801 acaatgagtg gcttgctggt agctgccaca gcttccaaca gagcgcgctg cgccgcgggc
     7861 agggcgatgt ctgtgcggtc gcctccggaa aacccgggaa tgtgcaccgg catctcttcg
     7921 ccttccacct cagaggagag ccccacgaaa gccacaacca catcggcctg ctttgcggct
     7981 tcgacagcct gctgacgcaa ctgtgtttca tcgggtttcc acttgagggt gatgcccgcg
     8041 ccgtagtccg tgcccttgcg cgagtacttc atctcaaacg gatgtggctt cgcatcgccg
     8101 aaatgcacag tgaagctgag cggcctgttg ttgtggcggg gattgtcatt cgtaacactc
     8161 gcaacctgct tgccgtctac ggaaacgctg tacgcatacg gagcggcaca gtaaggctcg
     8221 caccttgccg gttgcacggt gaaggtcacg tctccgggag caagcggctg aaagctgccg
     8281 ctccagcgca cgctgaattg cttcgcactg cgtacgggga ctggcgccac gcggttccag
     8341 tcaaaatcaa tctgcgcatc acgctgtgtt accggagtgc cgctgaaatc cgcattcgaa
     8401 aaatattccg cggtcagcgc gcccggttga ccatccttat cgcggaaaac attgcttggc
     8461 acaaccacag gaagctctgc tactaacggt gctccctgcg aatagagcac cttcgcggat
     8521 ttgaacttct gttcgatgcc ttcgagcgga gtcacgggat gcagcggcgc cccgttgtag
     8581 tttccttcca gagtgatcag cgactcggca ttcgggccta ctacggcaat ggtcttgaca
     8641 gcgttcgaga gcggcagcgt gttgtgctcg ttcttcagca gcacaatgga ttcctgcgcc
     8701 gccttcagcg ccagttcggc atgttccgga gtggcatttt cgcccggcga aatacgtgaa
     8761 taaggatccg accccggcgg atcaaacaat cccagcttga agcgcgctgt aaagagccgc
     8821 tccactgcct gatctatggc cttttcatca atcaggtgct gatgcaccgc atccaccagc
     8881 gcagcgtatt ctgtgccaca actcaggtca tcgcccgctt ttacagcagc ggctgaagcg
     8941 tgggccgcat ccggtgagaa gtggtgccca ttcgcactaa agaagtcgct gatggccccg
     9001 cagtcggaaa ccacatagcc ctgaaagccc catttctttc gcagcgtctc atccagcaga
     9061 aacttgttcg cgcaggcagg ctcgccattc acgctattgt aggcgcacat tatggagccg
     9121 gccttcgcat ccaccatagc catgcgaaag gcaggcaagt aggtatcttc aacatcgtgc
     9181 ggcaaaggat caatattggc ttcatgcctc gtggattccg gcccgctgtg caccgcgtaa
     9241 tgcttggccg ttgcgatggt cttgaagatg cgcggatcgt cgccctgcat tccacgaatg
     9301 aaggccgcgc ccatctgccc ggtcagaaaa ggatcttcgc cataggtctc ttgtcctcgt
     9361 ccccagcggg gatcgcgaaa gatattgatg ttaggcgacc agaaatccag tccgcgatag
     9421 atgctgtggt cgccctcggc ctgcgcctcg ttgaactttg ctcgtgcttc ggtcgaaatg
     9481 acgcttgcga tgcggtgttc caggtccgtg tcccatgtgg cggccagccc gatggcctgg
     9541 gggaagaccg tggcatagcc cgcgcgcgcc actccgtgca gcgcctcgct ccaccagtcg
     9601 tattccggaa cacccagccg cggaatggcc actgcatggt tctgcatctg cgaaactttt
     9661 tcttccagcg tcatgcgcga aaccagatct gccgcacggc gttccgctgg ctgagacggg
     9721 tcaagataga gcggcttctg tgcgttagac tggcagaaac cggagagagc ggacatcaaa
     9781 gcgacggtag cagagagaca gagttttgtg gccttcatag gaatcctgca acagtgtaca
     9841 aaagcgattt tctttgtttc tgatttgaaa taatgtttct attaaattac catcagggca
     9901 accaggcgag gcaattgtag tgcgaatttc actcttcatc acctgttaca acgacaccct
     9961 cttccctgag acgggaaagg cggtcgtcca attgcttgaa cggctcggcc atgaagtgga
    10021 gttcgcgctg gagcagacct gctgcgggca aatgcactac aacaccggat atcagcagga
    10081 agccatgccg ctgatgcggc gcttcgttga aattttcggg gacgctgaag ttgtctgcat
    10141 tccatcttca tcatgtgtgg ccatgattcg tgatcactat ccgaagatgg ccgaagcaac
    10201 aggcaatgcc gcgctgatca aagcggtcga agacctgctg ccgcgcgtct atgagctttc
    10261 tgagttgctg gtaaacaagc tggggctgga agacgtcggc gcgtactacc cgcatcgtgt
    10321 gacgtaccat gcaagctgcc actcgctacg cagcctccat cttggcgatc tgccactgag
    10381 actgcttcgc aacgtgcgcg gcatagattt agtggagctt caggatatag accagtgctg
    10441 cggttttggc ggcaccttcg cagtgaagat gggcgatgtc tccgccgcta tgctggctga
    10501 caaaatccgc agtgttgaag ataccggcgc cgaggtgtgc acggccgtgg acaattcctg
    10561 cctcatgcac atctttggcg gactgcaccg cctgcgcgcc ggagtgaaga ctgtgcatct
    10621 ggctgagatt ctggcgtcca ctgaagaagg gatggcgcga tgagcattcg cgtcggacaa
    10681 gcttccgaag cgccgccctt tcagaaggcc gccctgccgc tgctcgatga cacacagctc
    10741 cgcaaaaacg tacgtcatgc cacgaatgtc atcacacgca aacgcgccgt ggttgtaggc
    10801 gagcagcagg actggcaaca actgcgcgca tcggccagcg ccataaaaga gcacacgctg
    10861 cgccatctcg accactatct cgaacagttt gagaaaaact gtactgctgc gggaggccat
    10921 gtccactggg ccgccgatgc cgacgaagcc aaccgcatca tcgtggacct gatcaggcag
    10981 catggcggca aagagatcat caaggtcaag accatgacct cagacgagat cggcctgaac
    11041 cgcgcgctgg aagcgaatgg catccatccg catgaaaccg accttgccga catgatcatc
    11101 cagctggggc acgacaaacc ctcgcacatt gttgtgcctg cgctgcacaa aaaccgccag
    11161 caggtgcgcg agattttcat gcaggccatg ggcttaaagg agcttggcga ccggcccgag
    11221 gacctgacca ccgctgcccg caattatctg cgagaaaaat tcctgaaagt gaagatcggc
    11281 ttcagcggag cgaattatct cattgcggaa accggaggag tctgcgttgt cgagtccgaa
    11341 ggcaacgggc gcatgtgtct cacactgccg gacacactta tcaccattgc cggaatcgaa
    11401 aaagtcattc ccacattccg cgacctcgaa gtgttcctgc aactgctgcc gcgctccgca
    11461 accggcgagc gcatgaaccc gtacaactcc ctctggactg gcgtgcataa aggcgaaggc
    11521 ccgcaggagt ttcatgtcat cctgctcgat aatggccgca cgcgcatcct ctctgaccac
    11581 gaatcacggc agaccttacg ctgcattcgc tgtggagcct gccagaacgc ctgcccggtc
    11641 taccgccaga ccagcggtct ggcctacggc tccatctatg ccgggccgat cggcgcgatc
    11701 cttacaccgc agctctttga catgcagcac gcgcagactt tgccttacgc atcctcactc
    11761 tgcggggctt gctatgaagt gtgcccggtg aagatcaata ttcccgaagt gctgattcac
    11821 ctgcgcggac gcgttgtccg tgaacagcag tccagtgcaa acctcgaagc cctggggatg
    11881 aaggcgatgt cgcgcatttt cctgagccgc cgcagctttg aagcagcaca gcgcctgggc
    11941 cgtatcggcc aatggccatt cgtttccgat ggctgggtca ggcatcttcc aggaatgctg
    12001 ggaaactgga ctgccgtacg cgaccttcgc cccatgccga agcagacatt ccgcagttgg
    12061 tggcaggaac gaaagaaaac ataaaatttc atggcccata caagcctggt gttggcttgt
    12121 gcggggtaat ggaccagaaa gtcacaatgc cagaaacaaa cgcacgcgaa gtcatcctcg
    12181 accgcatccg ccatgcgaat gctgatgcag ctccatccga gtactcggtc atcgagcgca
    12241 cctatcggca aaaggccggt atggaccgtg cggaaatcca gaaggcattc gccgaacgtc
    12301 tgcatgaata tgacgcgcac gttacagaag ccaccgcgga aacactgtct gctgccattg
    12361 ccgaaagtct tcgccgcaac aaccaaaaat ccgctatcgt tgccagcggc ttcccgcagc
    12421 agctcctgcc tgaagggttt ctctggcagc aggaatccag cgccacgaca gagcagctca
    12481 acaccgcgga aggagcaatc tgcgcatgcg aggtagcgat tgcacatacc ggcaccatcg
    12541 tcattcgaaa cgcgcgaaga ctcacgctgc ttccagaccg cctgttgtgt gtagtgtatg
    12601 aagaccaggt tgtcgagacc gttcctgaag cctttgcccg cttgcagcct ttcgcatccg
    12661 aaccgctcac gtttatctcc ggcccttcag ctacagccga catcgaaatg acccgcatac
    12721 gaggcgtgca tggcccgcgc tttctggatg tggtgctggt ccgtaattca caataggaac
    12781 catgaacgtc tgcgcgcaaa tcgtgctcag cgctacccat tgttttcagg ccaaagtgta
    12841 aactagtatt gagaaactta tgcccgaaat acaacgcaga aagactgtta ctgtcaggat
    12901 tgggaatgtt cgtgtcggct cggaagcccc tgttgttgtc caatccatga ccaacactga
    12961 cacctccgac gtgcaggcga ccatccagca ggtggccgcg cttgcagccg ccggatcaga
    13021 gctggtgcgc gtaacggtga acaatgatga tgctgcgaag gccatccctt atatcgttga
    13081 aggtctggca aagatgggta ttcacgttcc catcatcggc gacttccatt acaacggtca
    13141 cattctcctc aagaagtatc ctgactgcgc caaagcgctg gcgaagtacc gcatcaatcc
    13201 gggcaatgtt tcgattggac gcaaagacga cgaaaacttt cgcaccatga ttgagtgcgc
    13261 tgttgaaaac aacaagcctg tgcgcatcgg ggtcaactgg ggttcgctcg atcaggccct
    13321 gctgacgcgc ctgatggacg agaattcaaa gcgccccgat ccgctggacg cccgcgacgt
    13381 gatgatggaa gccatgtgcc gcagcgcgct cgattctgct gctgccgctg aacgttacgg
    13441 cctcggtcac gacaagatca tcatcagcgg aaaggtgagc ggcgtacgcg acctgattga
    13501 tgtgtaccag atgctcgccg cgcgctgcga ttatccgctg cacctgggcc tgaccgaagc
    13561 gggaatgggc atgaagggca tcgtcgcctc gaccgccggg cttgctccac ttttgctcaa
    13621 gggcataggc gatacgattc gtgtttcgct cacgcccaaa ccggggggcg accgcacgga
    13681 agaggttctg accgcgcagc agattctgca atcgcttggc atccgcagct tcaccccgca
    13741 ggtcaccgcc tgccctggtt gcggacgcac cacgagcaca tttttccagg agcttgcgca
//
LOCUS       DASRBV010000083        13287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_409866, whole genome shotgun sequence.
ACCESSION   DASRBV010000083 DASRBV010000000
VERSION     DASRBV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409866"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFA02_03445"
     CDS             complement(<1..194)
                     /locus_tag="VFA02_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU08931.1"
                     /translation="MKQKSTKPAGIKEIAAALNISIGTVDRALHDRPGVSPKTRAKVM
                     KMAEKLNYRPNVAARSLKLNR"
     gene            444..3983
                     /locus_tag="VFA02_03450"
     CDS             444..3983
                     /locus_tag="VFA02_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU08932.1"
                     /translation="MLRLLYGGPSLRTIRFTGWSAILMVLCVLCVLPACAQMDQGTIT
                     GTVTDATGAVVPGAEVTLISTDTGLVLKTTTNQSGVYVFSPLKIGNYSVSASAAGFAT
                     SNISGLVLNVNQRLAADLKLQPGNVSQTVTVNSGVEQLLQTQQSSTGQVVSAQVINDT
                     PLAGRNYVFIAQLTAGVAQSNGSRGLGKGDFSANGLRAEQNNFILDGVDNNSNAVDFL
                     NGASFVVKPPPDALAEFKVQTSTYDAEFGHSAGAVLNASIKGGTNSLHGDVWEYWRND
                     ALTAKDFFAQTTPEYRQNQFGGTLGGPIIRNHLFFFGDVEATRIIIGQTGTYTVPTQS
                     MRNGDFRELLNPALTGATGPITLYQPGSAGTALLACNGQQNVFCANQINPIAQRILNL
                     YPQPNANGGKTYNNYIINRKNADNTVSWDGRLDWNATANDQAFFRMSYYNERGNYAPP
                     LGPILDGGSYGSDGPTINMGENYALSETHEFNNALANEFRFGYNWGHFQFLQPNANNN
                     IAPTLGLGGIPYQKNNGGLPSTGVSGISSFGSPGFYPAIEYENVFQVLDNVTKITGNH
                     TLKMGVAFQHVRYSTTAPTSPHGSYTFNGFYTSKPGVTNTGSGVADFMADQMNSASIT
                     NFFNIDNVRWYNSGYFQDDWKVLPNLTLNLGVRYDYYQPPEERHDNQAVWYPTAITAP
                     GSGTANYVMAKSKQDLPLAPIFLNLIAKDNINLVYTGDRSILKPEYTDISPRVGFAYT
                     PFSRFVIRGGYGIFYGGLESIGGAPNPGFNYPFQFSSNFTAPGCSATNCPTNGLTLEA
                     GFSQALAQGIQNFLSTPSLVGSQPKFHSSYTEQFNLSTQYAFSPSLSLTLAYVGNLSR
                     RLQAFPDQNAPYGLLGPKDNAQRIRPFPDFGGSQFNAFEGVGSYNSGQATLEKRMSNG
                     LYFLATYTYGHALDDTATPLRGGSNTYRNALLIPMGYELASSDWDVRHRFTFNGNYQL
                     PFGRGRRYLNQGGVLNTLVGGWATDLVFVAQTGTPFTVSPNNPGVNGASRRALLKRDP
                     FASGGSQDPTNPGTTCATNTKNIQHWFNPCAFANPLSGGDIPNTQTAQNPLGNPIRGL
                     AQVLAYTGSARNQIHGPGYERINASVFKNFDTFEGQYLQFRADVFNLFNTPAFGDPSG
                     SINPNGGQITGTRNLGLFTPNARFIQLALKYYF"
     gene            4005..6200
                     /locus_tag="VFA02_03455"
     CDS             4005..6200
                     /locus_tag="VFA02_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZU08933.1"
                     /translation="MRRILMAVLILAAAPLASGAQTATSTAANTASDVCVQQQSAPVL
                     PLQTSGRYIVDSTGRRFKLSGAAWYGAEGTDFVVAGLQVEPLAAIVRHIRCMGINTVR
                     MLWSNEMYESNPIVPDYALAANPQLKGKHALQVFDIVVRELARQGILVILDNHNSNAE
                     WCCGNDGNQLWYNSQYPESSWIADWKAMVARFKDVPQVIGADLRNEPRISATWGGDPA
                     TDWHAAAERGGNAVLSVNPNLLIIVEGVNYALDLTGAANLPIQLNVANRLVYSAHDYP
                     FDHNGATTYAALKQSWDQQWGCLLTTTPVWIGEFGNCHNQSICLSDNNPGDGSGGFWF
                     QSFRTYLDQNDIDWSYWPLNGTEASGQGRTYNGEEGYGMLNPYWNSPNTPSELNPPPA
                     INTLGVMQPIMAPNQGPKLLASYPPVITITQPLPGTTIASGYPLALSANAALRSGSSD
                     SITKVNFYANGNLIGATTTAPYSITWQNIAPGSYQIQAEAVTAAGVKTRSETVPVLAI
                     NYATHPPAYSSSIAVNFVSYAVTPMASTEVAGVVPQPNWNQALGNTNSGTLTGLVDQN
                     GNATTASVTWTSPNTYSTAIPDQPGNNRMMKGYLDNNNVQPNTVQVAGLPSSFKNYDV
                     IVYFDGGNEGTNPPARAANYRLTTLENGQIHGCAGQTREGSTVSGLDAAGTDFSGTFI
                     QASAGSAGNYVEFVNCTGDNFSLAPVHGGSTDTQYRAPVNGIQILARSR"
     gene            6569..7582
                     /locus_tag="VFA02_03460"
     CDS             6569..7582
                     /locus_tag="VFA02_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU08934.1"
                     /translation="MNIKEVARLAKVSTATVSRTINGSNKVTPETANRVRKAIEALRY
                     YPNTNARALGSGRSSLYGLIISDITNPFFPELVKSFEDIAVQHGQEVLVANTNYDPHR
                     TEICVGRMLQRKVDGVAIMTSEMDKHLIEEFSHRNIPLAFLDTATPQKGISNIRVNYA
                     AGIDAAVEYLLGLGHTAIGFIAGPTSLSSARIRKRAFLNIMKRKGIQVDDRFLEQGNH
                     RTDGGHEAMLRLLNQSQRPTAVIASNDLTAIGAMGAIFEHGLRIPQDISLIGFDDIEL
                     CAFTQPALTTVRLSRQEIAKLAFRTLYNTHQLENWDGAEYVVQPVLVVRKSTGPVPEK
                     RWS"
     gene            complement(8117..10339)
                     /locus_tag="VFA02_03465"
     CDS             complement(8117..10339)
                     /locus_tag="VFA02_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU08935.1"
                     /translation="MKFVRKFIVSSGTLRLVIATSFFAVSVFCFHKTIHAGANPEAVR
                     SEYNAEVHKGYDYHFGKDKPFLPSNATTEDGEFIQPGAFPSAKYCGHCHEEAYHEWRE
                     SLHSNSFRAPFYLKDVQLLNKTQGIEFSRHCEGCHNPTALFTGALTKNTVVKDRSFDN
                     DGITCSVCHSIQKLQPTYGVGSYVMGVPSVMVDENGKPIPGEVPYKDILAHTDRHVQA
                     VMKDFYRTPEYCGACHKANLPTSLNHYKWLRAIGLYDEWQASSFSHRSPLPFYSKEYK
                     TCQDCHMPREAITRPDYGAKDKTLASHRWIGGNTAVPFFYGYEEQLQKTIAFLRDQKL
                     NVDLFAIRKNNDPKYIAPLGSVDFDLKPDDTVETFVVIQNKGIGHTLIPEQRDIYEAW
                     VQFTVKDASGRVLSQSGYLQPDGTLEPRAHSFITRMLDKKGNLLIKHEVWLRHTNATD
                     ATIRPGRSTIVRYQFRVPADAKGPLTVTAKVNYRHLNQVYTNFILGDKHPLYPVVEMA
                     SRTRTFEIGHNGAGKPDPSDNPDWMRWNNFGIALMDQQQYGDAVAAFEEVRKMRPDYA
                     DVYTNIGIAYLSWEKYALAGESIRKALELSPDNPRALYYQALVERNEGNLDHAIANLE
                     KVVAKYPQSPDAHRELGFSYYQLHKYELAEKQYQALQAIDPDDLAAHYILAIVYRRLG
                     MKEKASEESARFADEKEDPMANTASLQFLEKHPELSGESVPWHLHTALDAAPMTASGK
                     "
     gene            complement(10500..11204)
                     /locus_tag="VFA02_03470"
     CDS             complement(10500..11204)
                     /locus_tag="VFA02_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZU08936.1"
                     /translation="MKATILQASMGVDYRPDKGEAMNVTERAQVRRVPKRAAYDAETI
                     YSILGAGFLAHVGFCVGGQPFVIPTLYGRNGNKLYLHGSAASRMVRELENGVPACVSV
                     TIVDGLVLARSAFHHSMNYRSVVAFGTARKIEGNEQKTEGLRVISDHLLAGRWDDVRS
                     PYEKELKATSVLEFSIEEASAKIRTGPPVDEEEDYALPVWAGVLPLELKSDAPVADSR
                     LAEGMAVPDYVLRFRR"
     gene            11217..12650
                     /locus_tag="VFA02_03475"
     CDS             11217..12650
                     /locus_tag="VFA02_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZU08937.1"
                     /translation="MELLIPLSERNGPLYRQIYLSLRQAILSGTLRPGERLPSTREMA
                     EKLHVSRTVVLLAYDQLLAEGFLTGRHGSGTFIAEGLSLRQRKESTRPAKLRLSNFGR
                     YAAETVSPIAHSGRRAQSLRYDFAYGQTDTDIFPFAAWRRTLLRNERRTVLRDLDYGP
                     ATGNQNLQEAIAAHVRKSRAVACDPSQVIIVNGAQQAIDLIVRVLLARGDRVAIEDPQ
                     YRGARHALLAAEARLCPVPVDNDGIVTGKLPDRARAAFVTPSHQLPTGAVLTLSRRLE
                     LLAWARRQNAVIVEDDYDGEFRYEGQPLESLQGLDPEGRVIYIGTFSRTIFPSLRIGY
                     LIAPKSLVPAISAAKWLCDRHTGTLEQRTLAEFISSGMYERHLHQVRKRNTLRRTALL
                     DAIAKYIGNGAEITGEGAGAHIVLWPNRRLDEEIIIRKAAAQNVGIYGIAPYFLRTPS
                     RAGFMIGYSRMKEADIREGIKRLGNVL"
     gene            complement(12657..>13287)
                     /locus_tag="VFA02_03480"
     CDS             complement(12657..>13287)
                     /locus_tag="VFA02_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08938.1"
                     /translation="DTHLEKGPSTFDVAHAFNLSVVQDLHLDEVEFLEPITKKLTGGW
                     QLLSISSINSGPPFTVYSGIQQTGAGSNGADRPDQIAKPHLSSARKVREDYFGRGKDN
                     ASFFFVPVNLFGGTGPNSGRFGSLGRNTFRGPAYYNYDFALIKDTPFGTRPSGSEMMD
                     LQFRAEFFNLFNIVNMGLPANIIAGTGFGEISKTAGNSRQIQFSLKLIY"
BASE COUNT         3123 a         3998 c         3274 g         2892 t
ORIGIN      
        1 cggttcagct tcaggctgcg cgccgcaaca ttgggccggt aattcagctt ctcggccatt
       61 ttcatgactt tggcgcgggt cttggggctg acccccggcc tgtcatggag cgcccggtcc
      121 accgtgccaa tggaaatgtt cagcgctgcc gcaatttcct tgatgcctgc cggctttgtg
      181 cttttttgtt tcacgacgtg tatccatttt agccgcgcca gaaacgcttt cttcggagaa
      241 aaaagaagca aatatctagc aaacgtacac gcagtctatc cggcggcccc aggcttcagc
      301 gtaaatgtcc ctgttttccc tataaaaata tggaaataat gtcttgacaa ttatttttat
      361 gggagcgtac cgtgtacgac tcaagagaaa atttcgtgaa cgtacacgat attattactt
      421 cacgatttac gaggagaaag attatgctgc gtttgcttta tgggggtccc agtctgcgga
      481 ccattcgctt taccggatgg agcgcaattc tgatggtgct atgtgttctt tgcgtcctgc
      541 ctgcctgtgc gcagatggac cagggcacta tcaccggcac agtgacagat gcgaccggtg
      601 ctgtcgttcc cggcgcggag gtcacactga tcagcacaga taccggactt gtactgaaga
      661 ccacgacaaa tcagagcggc gtctatgtct tctctcccct gaagatcgga aattactctg
      721 tttcagcatc tgctgcggga tttgccacga gcaacatttc cggcctggtg ctgaacgtaa
      781 accagcgcct tgccgctgat ttgaagctgc aaccaggaaa tgtttcgcag accgtcactg
      841 tgaactcggg cgtggagcaa ctattgcaga cacagcagtc ttctaccgga caggtcgtca
      901 gcgcacaggt gattaacgac acgccgctcg ctggacgcaa ctatgtcttc atcgcacagc
      961 ttaccgccgg tgttgcacag tcgaacgggt cgcgcggact cggtaaaggc gacttcagcg
     1021 caaatggcct tcgcgccgaa cagaacaact tcatcctcga tggagtagac aacaactcga
     1081 atgccgtaga cttcctgaac ggcgcaagtt ttgtagtaaa gcctccgcca gacgcgctgg
     1141 ctgaattcaa agtgcagacc agcacctatg acgcagaatt tggccactcg gcaggcgcgg
     1201 tgctcaatgc ttccattaaa ggcggcacca actccctgca tggcgatgtc tgggaatact
     1261 ggcgcaatga cgcgctcaca gcgaaagact tttttgccca gaccactccg gaataccggc
     1321 agaaccagtt tggtggcacc cttggcggac ccatcatcag gaaccatctc ttcttctttg
     1381 gcgatgtgga agccacccgt atcattatcg ggcagacagg cacgtatacg gtgccgacgc
     1441 agagtatgag gaatggggac ttcagagaac tgctcaatcc cgcactcaca ggggccacag
     1501 gacccatcac tttgtatcag cctggttctg ccgggactgc tttgctggcc tgtaatggcc
     1561 agcagaatgt cttctgcgcc aaccagatca acccgattgc gcagcgcatc ctcaacttat
     1621 atccgcagcc aaacgcgaat ggcgggaaga cttacaacaa ctacatcatt aaccgcaaaa
     1681 atgcagacaa cacagtttca tgggacggcc gccttgactg gaatgccact gcgaatgacc
     1741 aggcgttctt ccgtatgagc tattacaacg agcgtggcaa ttatgctccg ccgttaggcc
     1801 ccattctcga tggcggcagt tatggctctg atggaccgac catcaatatg ggcgaaaatt
     1861 atgcattgag cgaaacgcat gagttcaaca atgctttggc caatgagttt cgcttcggct
     1921 ataactgggg acacttccag tttctgcaac caaacgcaaa caacaacatt gcgcccacgc
     1981 tcggactggg cggcattccg taccagaaga acaatggcgg cctgccaagc actggtgttt
     2041 ccggaatcag cagctttgga tcaccggggt tctatccagc cattgaatat gaaaatgtct
     2101 ttcaggtcct cgataacgtg acgaaaatta ccggaaacca cacgctgaaa atgggagtgg
     2161 ccttccagca tgttcgctac tcgaccactg ctcctaccag cccgcatggc agttatactt
     2221 tcaatggttt ttataccagt aagcccggcg tcaccaatac tggatccggt gtggccgact
     2281 tcatggccga ccagatgaac agcgcaagca tcaccaattt cttcaatatt gataatgtgc
     2341 gctggtacaa ctcaggctac ttccaggacg actggaaggt cctgcccaac ctgacgctga
     2401 acctcggcgt ccgttacgac tactatcagc caccggaaga acggcatgac aatcaggccg
     2461 tctggtatcc cactgccatc accgcacctg gctctggaac ggcgaactat gtcatggcga
     2521 agagcaagca ggacctgcct cttgcgccca tcttcctgaa cctgattgcc aaagacaata
     2581 tcaatcttgt ctatacggga gaccgctcca tcctgaaacc ggaatacacc gatataagcc
     2641 cacgggtcgg gttcgcctac actcctttca gccgttttgt gattcgcgga ggctatggca
     2701 tcttctatgg gggactggaa agcataggag gcgcgcccaa cccgggcttc aactatccgt
     2761 tccagttctc gtcgaatttc acggcaccag ggtgcagcgc aaccaattgc cccacgaacg
     2821 gtctgacact cgaagccgga ttctcgcagg ccctggcgca gggcattcag aacttccttt
     2881 ccacgcccag cctggttgga agccagccaa aattccactc ttcttataca gagcaattta
     2941 atctttcaac ccaatacgct ttctcgccgt cgctcagtct cacgcttgct tatgtaggca
     3001 atctctcgcg ccggttgcag gcgtttcctg atcagaatgc tccttatgga ctgcttggcc
     3061 cgaaggacaa cgcgcagcgc attcgtccct ttcctgattt cggcgggtcg caatttaatg
     3121 cgtttgaagg cgtaggcagc tacaactcag gacaggccac tctggaaaag cgcatgtcga
     3181 atggcctgta tttcctcgcc acctatacct atggccacgc cctggatgac acagcgactc
     3241 cgctaagggg tggctcaaat acttatcgca atgccttgct gattcctatg ggctatgagt
     3301 tggccagttc ggattgggac gtgcgccacc gcttcacctt caatggcaac tatcagcttc
     3361 cgttcggcag aggacgccgc tacctgaacc agggcggcgt tctgaatacg cttgtaggcg
     3421 gctgggccac ggatcttgtt ttcgtcgccc aaaccgggac cccatttacc gtaagcccca
     3481 acaacccagg cgtcaacgga gcatcccggc gggcactcct caaacgcgac ccgttcgcaa
     3541 gcgggggatc gcaggacccc accaatccgg gcaccacctg cgctaccaac acaaaaaaca
     3601 tacagcactg gtttaatccc tgtgcctttg ccaaccccct ctccggcgga gatattccga
     3661 atacgcaaac tgcacagaat ccgctgggga atcccatccg tggacttgct caggtcctcg
     3721 catacaccgg aagcgcccgc aaccagatcc acggccctgg atacgaacgc atcaacgcga
     3781 gcgtcttcaa aaacttcgac acctttgaag gccagtatct acagttccgt gcagatgtct
     3841 ttaacctctt caacacccct gcattcggcg atccgagcgg cagcattaat ccgaatggcg
     3901 ggcaaatcac aggaacacgt aatcttggtt tgttcacgcc aaatgcaagg ttcatccaat
     3961 tggcattaaa gtattacttc tgacaaaaaa gcacgggaga acatatgcga cgcattctga
     4021 tggctgttct cattctggct gcggccccac tcgcatccgg ggcgcagacc gcaacctcca
     4081 cggccgcaaa cacggcatct gacgtttgcg tccagcagca gtccgctccc gtgcttcctc
     4141 ttcaaaccag cggacgctac atcgtggaca gcaccggcag gcgcttcaaa ctcagcggcg
     4201 cagcatggta cggcgcagaa ggcaccgact ttgttgtcgc cggattacaa gtggagccgc
     4261 ttgccgccat cgtgcgccac attcgctgca tgggcatcaa tacggtccgc atgctctggt
     4321 cgaacgagat gtacgaaagc aatcccatcg tgcctgacta tgcccttgct gccaatccac
     4381 aactcaaagg caagcatgcc ctccaggtct tcgatatcgt cgtccgcgag cttgcccgcc
     4441 agggaattct cgtcatcctc gacaaccaca acagcaacgc cgaatggtgc tgcggaaacg
     4501 atggcaacca gctctggtat aactcgcagt accccgaatc aagctggatc gccgactgga
     4561 aagccatggt cgcgcgcttt aaagatgtgc cccaggtcat cggcgccgac ctccgcaatg
     4621 agccgcgcat ctccgccacc tggggaggtg accccgccac cgactggcac gccgcagccg
     4681 aacgcggagg caacgccgtc ctttccgtta accccaacct gctcatcatc gtggaaggag
     4741 tcaactacgc gctcgatctc actggcgcag ccaatctccc cattcagttg aatgtggcca
     4801 acagactcgt ctactccgct cacgattatc ccttcgacca caacggcgca accacctacg
     4861 ctgccctcaa acaatcgtgg gaccagcagt ggggctgttt attgacgacc actccggttt
     4921 ggatcggcga atttggaaac tgccacaatc agtccatctg cctaagcgac aacaatcctg
     4981 gcgatggctc cggcggcttc tggttccaga gctttcgcac ttacctcgac cagaatgaca
     5041 ttgattggtc ctactggccg ctgaacggca cggaagccag tggacaaggg cgtacctaca
     5101 acggagaaga aggctacggt atgctcaatc cttactggaa ctccccgaac actccttcag
     5161 agttaaatcc gcctccggcc atcaacacat tgggcgtcat gcagcccatc atggcgccca
     5221 atcaggggcc aaagctgctc gcatcctatc cgccggtcat taccatcaca cagccgctgc
     5281 ccggaacaac gatcgcttca ggatatccgc ttgccctcag cgccaatgca gcattgcgct
     5341 caggcagtag tgactctatt accaaagtga atttctatgc caacggcaac ctcatcgggg
     5401 ccaccaccac agcaccatat tccatcacgt ggcagaacat cgctccagga agctatcaga
     5461 tacaggcaga agctgtgact gctgcgggcg taaaaacacg ttctgaaaca gtacccgtcc
     5521 tggccatcaa ctacgccacg catccgcccg cctattcatc ttccatcgcc gtgaatttcg
     5581 tcagctacgc cgtcactcct atggcctcaa ccgaagtagc aggggtcgtt ccccagccca
     5641 actggaacca ggcattaggc aacaccaact ccggcactct caccggactt gtggaccaga
     5701 acggcaacgc caccactgca tccgtcacct ggacctcgcc caacacatac tccaccgcta
     5761 ttcctgacca gcccggaaac aaccgcatga tgaagggcta cctcgataac aacaacgtgc
     5821 agccaaatac tgtccaggta gcgggcctgc cctccagctt caaaaattac gatgtcattg
     5881 tgtatttcga tggcggcaat gaaggcacga acccaccggc gcgcgcggcc aattaccgcc
     5941 tcaccactct ggaaaatggg cagatccacg gctgcgctgg gcagacccgc gaaggcagca
     6001 ccgtctccgg tctggatgcc gcgggcacag acttctccgg aacattcatc caggcctcag
     6061 ccggttctgc aggaaattac gtcgagttcg tcaactgcac tggagacaac tttagcctcg
     6121 ccccggttca cggaggctcc acagacacac aatatcgcgc ccccgtaaac ggaatccaga
     6181 ttcttgcccg ctcgcgataa aagcgagaaa aaccgggtgg aaataagcat ttcgctgggc
     6241 cgggtgcccc atgtcttgaa aagatgtggg tttgcagcgt cttatggcct ttttcgtata
     6301 tggatagggt tccgatgcac atgctttcac aagacgatgg atagctgggt actggcaact
     6361 ggggacgaat tcttggcaca aagtccgtca tgctgacggc cgaagggagg aggaatcctg
     6421 gcgacctgcg ggtttgaccc aatattggca tcgggttttg tcccagtgaa atgacctccg
     6481 gcctgctgcg gaaacatatt caggagaaac ctccggaaca aattacaatt ctcttttgca
     6541 gtgattccat aatggaacca ggagctgcat gaacatcaaa gaagtagcca ggctggccaa
     6601 agtttccact gccacagtct ctcgcaccat taacggctca aacaaggtca ctccggaaac
     6661 cgccaaccgc gtgcgcaaag ccattgaggc cctgcgttat tatccaaaca ctaacgcgcg
     6721 cgccctcggc tcaggccgca gcagcctcta cggactcatc atttcagaca tcacaaatcc
     6781 cttctttccc gaactggtaa agagctttga agacatagcc gtgcagcacg ggcaggaagt
     6841 gctcgtcgcc aataccaatt acgacccgca ccgcaccgag atctgcgtag gccgcatgct
     6901 ccagcgcaag gtggatggcg tggccatcat gacctccgaa atggacaagc acctcatcga
     6961 agagttcagc caccgcaata tccccctcgc cttcctcgat acagcaacac cacagaaagg
     7021 catcagcaat atccgggtaa attatgccgc tggaattgat gctgccgtgg aatacctgct
     7081 tggcctgggc cacaccgcca ttggcttcat cgccggcccc acctcccttt cctccgcgcg
     7141 cattcgcaaa cgtgccttct tgaatatcat gaagcgaaaa ggcattcagg tagacgatcg
     7201 ctttctggag caaggcaacc accgcaccga cggcggccac gaagccatgc tgcggctgct
     7261 caaccagtcg caacgcccga cggctgtcat cgcttccaat gatctgaccg ccatcggtgc
     7321 catgggtgcc atctttgaac acggcctgcg tatcccacag gacatctcgc tcatcggctt
     7381 cgacgacatc gagttgtgcg cctttaccca gcccgctctg accactgtcc gcctctcgcg
     7441 ccaggaaatc gcaaagctcg ccttccggac cctctacaac acacatcagt tagaaaactg
     7501 ggacggcgca gaatacgtcg tccagccagt attggttgtc cggaaatcca ccggccccgt
     7561 tccggaaaaa agatggtctt aaaaaaatta tttgttctaa cgagtaatgt ccggaaatac
     7621 ctcccggatt tggtatcctg aaaataagaa gggtaatttg tacccaaagc ggactgcatg
     7681 acaagctcct cgtgattggc tgagatcgta tcaatccaac ttatttgttt tgaagaattt
     7741 acgaaaaagt aggagggctg tcacttccca ttcgccgcag aaaagcatca acagaaaacc
     7801 gccagcttcc gccccctgct tccagggctg aaacgggcct cttcaggccc agggtttaca
     7861 ttcctcgatg aaaaaggccg atgccaggca gatataaact ccctgctttg acgaatttac
     7921 gaaaaagtag ggggctgtca cttcccattc gccgcagaaa agcatcaaca ggaaaccgcc
     7981 agcttccgcc ccatgcttcc agggctgaaa aggtcctctt caggcccgaa gtttacattc
     8041 ctcaatgaaa aaggccgatg ccagacagat ataaactccc tgctttgacg aatttacaaa
     8101 aaagtaggga gtacctttac ttcccgctcg ccgtcatcgg agcggcatcg agcgcagtat
     8161 gcaggtgcca gggcacgctc tcgccggaaa gctccggatg cttctccaga aattgaagtg
     8221 aggcggtatt cgccatggga tcttctttct catcggcaaa gcgggccgac tcttccgacg
     8281 ccttttcttt catccccagg cgacggtaca caatggccag aatgtaatgg gcagccaggt
     8341 catccggatc aatcgcctgc aacgcctgat actgcttctc tgccagctca tatttgtgga
     8401 gctggtagta gctgaatcca agctcgcggt gcgcatccgg cgactgtgga tacttcgcga
     8461 ccactttttc cagattggca atcgcatgat caagattgcc ctcattgcgc tcaaccagcg
     8521 cctgataata cagggcgcgt ggattgtcag gcgaaagctc cagcgccttc ctgatactct
     8581 cgcctgccag cgcatatttc tcccagctca gataagcaat gccaatattg gtatatacat
     8641 cggcatagtc cggacgcatc ttgcggactt cttcaaacgc cgcaacggca tcgccatact
     8701 gttgctggtc catcagcgca ataccaaagt tgttccagcg catccagtcc gggttgtccg
     8761 atggatcggg cttgcctgca ccgttatgac ctatctcaaa ggtccgcgtg cgcgaggcca
     8821 tttccacgac tgggtacagc gggtgcttat cgccaagaat gaagtttgta tacacctggt
     8881 tcaggtgacg gtagttcacc ttcgccgtta cggtcagggg tccttttgca tctgccggga
     8941 cgcggaactg atatcgcaca atggtcgagc gccctggacg aatggttgca tccgtcgcat
     9001 tcgtatgccg cagccagacc tcatgcttga taagcagatt gcccttcttg tccagcatgc
     9061 gtgtgataaa actgtgcgcg cgcggctcca gagtgccgtc cggctgtaga tagccgctct
     9121 ggctcagtac acgccccgag gcgtccttca ccgtgaactg cacccaggct tcataaatgt
     9181 cgcgctgttc gggaatcagc gtatgtccaa tgcccttgtt ctggatgaca acgaaggtct
     9241 ccaccgtatc atccggcttc aggtcaaaat caacagaacc gagcggcgca atatatttcg
     9301 ggtcgttgtt tttccggata gcaaacaggt ccacattcag tttctgatcg cgcagaaagg
     9361 cgatggtctt ctgtaactgc tcttcatatc cataaaagaa cggaacagca gtattgcccc
     9421 caatccagcg atgcgatgcc agcgtcttgt cctttgcgcc ataatcaggc cgcgtaatcg
     9481 cctcgcgcgg catgtggcag tcctgacatg tcttgtattc cttggaatag aaaggcaatg
     9541 gcgaacggtg agagaacgac gatgcctgcc actcgtcgta cagcccaatg gcccgcagcc
     9601 atttgtagtg gttcaggctc gtcggcagat tggccttgtg gcaagcgccg caatattccg
     9661 gcgtgcggta gaagtctttc atcaccgcct gcacgtggcg gtccgtgtgc gccaggatgt
     9721 ctttgtaagg tacttcgccc ggaatcggct tgccattctc atccaccatc accgatggaa
     9781 cacccatcac ataggagccc acaccatagg tcggctggag cttctggata gaatgacaga
     9841 ccgagcaggt gatgccgtca ttatcgaagc tgcggtcttt gactactgta ttttttgtga
     9901 gcgctccggt gaacagggcc gttggattat ggcacccttc acagtgccgg gaaaactcaa
     9961 tgccctgggt cttgttcagc aactgcacgt ctttgagata aaacggcgca cgaaacgagt
    10021 ttgaatgcaa cgactcacgc cattcgtgat aggcctcttc atggcagtgc ccgcagtatt
    10081 ttgctgaagg aaaagcaccc ggctgaataa attctccgtc ctcagtcgtg gcattgctgg
    10141 gcaaaaaagg cttgtcttta ccgaagtgat aatcgtagcc cttatgaact tctgcgttgt
    10201 actcgctgcg gaccgcctct ggattggccc cggcatgaat tgtcttgtga aaacagaata
    10261 cggaaacagc aaaaaacgac gtcgcaatca ccagacgcaa tgttccgcta gaaacgatga
    10321 atttgcgaac aaacttcact ttttctggcc tgccaataat cctacgagag gtgcatcttg
    10381 aactttactg catcgcaggt acgacccaca agagagaaaa cctacgtata ttgctgaaag
    10441 caaaccagat acatcaaaaa gatgatacgt gtatccacct tttggtggat gccgcttctt
    10501 tatctccgga accgcagtac ataatccggc accgccatac cctccgcaag ccgcgaatcc
    10561 gccacaggag cgtccgattt cagttccaga ggcaacaccc cggcccacac gggcaaagca
    10621 taatcttcct cttcgtccac cggagggcct gtccggattt ttgccgaagc ctcctcgatg
    10681 gaaaactcca gcacagacgt cgccttcagt tctttctcgt acggactgcg cacgtcatcc
    10741 cagcgcccag cgagcagatg atcagagatg acgcgaagcc cttctgtctt ctgttcgttg
    10801 ccctcgattt tgcgcgccgt gccaaacgcc acgacggaac ggtaattcat ggaatgatga
    10861 aatgccgacc gcgccagcac caacccatcc acgatcgtga cactcacgca cgcaggcacg
    10921 ccgttttcca gttcgcgcac catgcggctc gccgctgagc catgcagata cagtttgttt
    10981 ccattgcgtc catacagcgt aggaatcaca aatggctgcc cacccacaca aaagcctaca
    11041 tgggccagaa agccggcacc cagaatcgaa tagattgtct ctgcatcata cgccgcgcgt
    11101 ttcgggaccc ttctgacctg agcccgctct gtcacattca tggcttcacc cttgtctggt
    11161 ctgtaatcca ctcccatgct cgcctgcaat atggtagctt tcaagaacca ctttttatgg
    11221 aattgctgat accactttcc gaacgcaatg gcccgctcta ccgtcagatt tatctcagcc
    11281 tgaggcaggc cattctctct ggaacacttc gtcccggcga acgcttacct tccacacgcg
    11341 agatggcgga aaaactccac gtctctcgta ccgttgtatt gcttgcgtat gatcagttat
    11401 tggccgaagg ctttcttaca ggccgccacg gctccggaac gttcattgca gaaggacttt
    11461 ccctgcgtca gcgcaaagaa agcacccgcc ccgcaaaatt gcgcttgtcg aattttggcc
    11521 gctacgcagc agagacagtt tctcccatcg cgcattctgg tcgtcgtgct cagtcgctgc
    11581 gctatgactt cgcctatggc caaactgaca cggacatctt cccgtttgca gcctggcggc
    11641 gcacgctact gcggaacgaa cgcaggaccg ttcttcgcga cctcgactac ggccctgcca
    11701 caggcaatca aaatcttcag gaggcgattg ccgcacatgt acgcaaatcg cgcgcggtcg
    11761 catgtgatcc atcgcaggtc atcatagtaa atggcgcaca gcaggccatt gatcttattg
    11821 tgcgcgtcct tctggcgcga ggtgaccgtg ttgctattga agacccgcag tatcgtggcg
    11881 cgcgccacgc attgcttgcc gcggaagcac gcctctgtcc tgtgcctgta gacaatgatg
    11941 gcatcgtgac cggcaagctc cctgaccgtg cccgcgcggc ctttgttact ccctcgcacc
    12001 agctgccaac aggggccgtc ctcacactca gccgccgtct tgaactgctc gcgtgggccc
    12061 ggcgccagaa cgccgtcatc gttgaagacg actacgatgg tgaattccgc tatgagggcc
    12121 aaccgcttga atcgctgcag ggactggacc ccgaaggccg cgtcatctat atcgggactt
    12181 tttcgcgcac catctttcca tcgctgcgta tcggatatct catcgcaccc aaatcgctcg
    12241 tccctgcaat ctccgcagcc aagtggctct gcgaccgcca tacgggaaca ctcgaacagc
    12301 gcacgctcgc cgagttcatc agcagcggaa tgtacgagcg ccatcttcat caggtgcgca
    12361 aaagaaatac attgcgccgg acagctttac ttgatgcgat tgcgaaatat attggcaacg
    12421 gcgctgagat taccggagaa ggagcgggtg cacacatcgt cctctggccc aaccggcggc
    12481 ttgatgaaga aatcatcatc agaaaggcgg cagcacagaa tgtaggtatc tacggaatcg
    12541 ctccctattt tctgaggacg cccagcaggg caggtttcat gatcggctac tcccgcatga
    12601 aggaagcaga cattcgcgaa ggcataaagc ggctgggaaa cgtgctctga aaagcatcag
    12661 taaatcagtt tcaacgaaaa ctgtatctgc cgcgaatttc ctgctgtctt gctgatctcc
    12721 ccaaaccccg tacctgcgat gatgttcgcc ggaagcccca tattcactat gttgaagaga
    12781 ttaaagaact cggcccggaa ctgaaggtcc atcatctcgc tgccactcgg ccgcgtcccg
    12841 aacggcgtgt ctttaatcaa agcaaaatcg tagttgtaat atgcaggccc gcgaaacgta
    12901 ttccggccca gcgagccaaa gcgtccgctg tttggccccg tgcctccgaa aagattgacc
    12961 ggaacaaaga agaacgaagc attgtccttc ccccggccga aatagtcctc acgtaccttg
    13021 cgcgcgctcg aaagatgcgg cttcgcaatc tggtccggac ggtctgcgcc gttcgatccc
    13081 gctcccgtct gctgaatccc tgaatataca gtaaacggcg gaccgctgtt gatgctcgat
    13141 atgcttagaa gctgccatcc acctgtcagt ttcttcgtaa tcggctccag aaattcaacc
    13201 tcgtctaaat gcaggtcctg caccacgctc aggttgaatg cgtgcgccac atcaaatgta
    13261 gacggccctt tctccagatg agtatca
//
LOCUS       DASRBV010000084         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_613037, whole genome shotgun sequence.
ACCESSION   DASRBV010000084 DASRBV010000000
VERSION     DASRBV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613037"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFA02_03485"
     CDS             complement(<1..539)
                     /locus_tag="VFA02_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZU08939.1"
                     /translation="MKRQKVVVLMVFFLGTISALVAYGKASASQFRQWRQTIVRTLFV
                     PAPLPPLSPDRHGSFAPAKGVIADKVTYGTEFGMRVPAVVYHPEKEKEKLPGMIVVNG
                     HGGDKYSWYSFYTGILYARAGAVVLTYDPIGEGERNPERKSGTRLHDRIVNAPNYPQK
                     LSGFMITDVMQAVSYLRTRP"
     gene            complement(536..2383)
                     /locus_tag="VFA02_03490"
     CDS             complement(536..2383)
                     /locus_tag="VFA02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZU08940.1"
                     /translation="MRRLFLALYLLTGPALISHAQLPAATQGPARTDLSGNWDFRFDP
                     KAEGEAAKWFTTNDQGTWQKAHVPGSFNVEFARDAGDTYRFYQGKVWYKTKFRGPDSL
                     RKDVFLHFSGTVLRQKVWLNGTYIGSSNLPWLDVSYNITKFIHRGPDNLLVVEVDNSV
                     LPNAIPDTKWRGWWDDGGLIWHVYLEERPRVRSEGFVTTTLQSDGEWLLGVHARVHED
                     APTKASVNVTLSKPGGQTIWQERRDVPPSADDVPVEINAVPKTIQAWSPEHPVLYRIT
                     LRTTAQGQPTDVISFRIGFRQIAVRDTRILLNGKPITLRGINRHEFAPGVGQSVPAAQ
                     NRKDLENIKALGANFVRLAHYSQSQDVYNDCDELGLLVWTEIPAWQTSAATLASPEVW
                     NDYAAPQLKAMIEQHRNHPSVIIWSVANEIPSDKPEAASYVAKAIDYVHSLDPTRLAT
                     FASDKRERDISMGPVDIIAVNEYYGWYYGKLDDVGPMLDKMHARYPDKPIIVSEFGSE
                     AVAHWSRETAKSGSKDYSYGYQVKLLSSHLAQIYAPMRRDYVAGGVIWVYSDFPDPHR
                     INGDHPDIARYRNSKGLVTADRVPKPAFATVQQFFRTLMREQQTGKGGQ"
     gene            complement(2396..>3365)
                     /locus_tag="VFA02_03495"
     CDS             complement(2396..>3365)
                     /locus_tag="VFA02_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08941.1"
                     /translation="YAYRCQGPDQRDFSPETETGNFDTKKGRARQDLLQFAENKPEDR
                     RVFAGIDEFVAEKLAGRADGRMGPFAVARIFADSAARTRILIHAMPHPTGRAAGEWRL
                     LRTDLLAASLLGDYYASRITGMTHLDFALHANSKQDYNIALRSLAQSRMAWKQLGETT
                     DAVYRPLSNPLRHQLEFTWSSQNAPLAELDATAEKFWMDRNTSSSHLPLTPKASDMKE
                     AGLYSASLESTTSGNTATVRCFLSSKQSASAVVLWWKPLPSELSWQSVPMRQVDHAWT
                     ATVPLNPRGLMYMVEVKHHEGEAENLPDETRETPYRIIQPFLRTRN"
BASE COUNT          739 a          879 c          923 g          824 t
ORIGIN      
        1 gggcgtgtcc gcagataact cactgcctgc atgacgtcag taatcatgaa gccgctcagt
       61 ttctgcgggt aatttggcgc gttcacgatg cggtcgtgca gccgcgttcc ggatttacgt
      121 tccggattgc gttcgccttc tccaatggga tcatatgtca gcactactgc gccggcacgc
      181 gcatagagaa tgcctgtata gaacgaatac cagctatatt tgtcgccgcc gtgcccgttg
      241 acgacgatca ttccgggaag tttttctttt tctttttcag gatggtatac gaccgcaggc
      301 acgcgcattc cgaactctgt accgtatgtc accttgtctg cgatcacgcc ttttgcgggg
      361 gcaaaactgc catgccggtc aggggagaga ggtggcagag gagcaggaac aaataaagtg
      421 cgcacgatgg tctgccgcca ttgccggaac tggcttgcag aagcttttcc ataagctacg
      481 agcgcggata ttgtgccgag aaagaagacc atcagcacaa ctactttctg ccgcttcatt
      541 gcccgccttt tcctgtctgc tgttcgcgca tcagcgttct aaaaaactgc tggactgtcg
      601 caaaggccgg tttgggaaca cggtctgctg taacaagccc tttgctgttg cgataccgcg
      661 caatgtccgg atggtcgccg ttgatccgat gaggatcagg aaaatcgctg tagacccaga
      721 tgactccgcc ggccacatag tctctccgca tgggcgcata gatttgcgca agatgcgaag
      781 atagcaactt cacctggtag ccgtagctat agtctttgct gccgcttttt gcagtttccc
      841 ggctccagtg tgccacagct tcagagccga actcgcttac gataatcggc ttgtccggat
      901 atctggcgtg cattttatcg agcattggtc caacgtcatc gagtttgcca taataccagc
      961 cgtaatattc gttcacggca atgatgtcca ccggtcccat cgaaatatca cgctcacgct
     1021 tgtctgaagc gaaagttgca agccgggtgg gatcgagact gtgaacataa tcaatcgctt
     1081 tggccacata agaagcggcc tctggcttat cggaaggaat ttcattcgcc actgaccaga
     1141 tgatgacact ggggtggttt ctatgctgct ctatcattgc cttcaattgt ggagcggcgt
     1201 agtcattcca tacttcaggc gaagcgagag tggctgcgga tgtttgccac gccggaattt
     1261 ccgtccagac caggagacca agctcgtcgc agtcattgta gacgtcctgc gattgcgagt
     1321 aatgcgcgag gcggacaaag ttggccccaa gcgccttgat gttttccagg tccttccggt
     1381 tctgtgctgc gggaacggac tggccgacgc ctggcgcgaa ttcatgccga ttgatgccgc
     1441 gcagtgtaat gggtttgcca ttcaataaaa ttcttgtgtc acgcactgca atctgacgaa
     1501 agccaatccg gaatgaaatt acatctgttg gttgaccctg cgcagttgtc cgcagcgtaa
     1561 tccggtaaag cacgggatgc tctggagacc acgcctgaat ggttttggga acggcgttta
     1621 tttctacggg cacatcatct gctgacggtg gaacatcgcg ccgttcctgc cagattgtct
     1681 gtcccccagg cttactgagc gttacgttca cagatgcctt cgtgggagcg tcttcatgca
     1741 cgcgcgcgtg gacaccaagg agccactcac catcagactg caatgttgtg gtaacaaagc
     1801 cttcgctgcg aacacgcgga cgttcttcga gataaacatg ccagatgagc ccgccatcgt
     1861 cccaccatcc tcgccatttt gtatcgggaa tggcattggg caggacagag ttgtccactt
     1921 cgacgacaag cagattgtca ggaccccggt ggatgaattt cgtgatgtta tatgaaacgt
     1981 caagccaggg cagattcgat gaacctatgt aggtcccgtt cagccatact ttctggcgca
     2041 gaacagtgcc cgaaaagtga agaaagacat ctttccgcaa gctgtccggg cctcgaaact
     2101 tggtcttgta ccagaccttg ccctgataaa aacggtacgt gtcacccgca tcccgggcaa
     2161 actcaacatt gaaagatccg ggaacatgcg ccttttgcca tgttccttga tcgtttgtag
     2221 taaaccattt tgccgcttcg ccttctgctt ttgggtcaaa gcggaagtcc cagtttcctg
     2281 ataaatctgt gcgcgccggc ccttgggttg cagcaggcaa ctgcgcatgt gaaatgagcg
     2341 cggggccggt gagcaaatag agagcaagaa agagtctgcg cattttgtgc ctgtgtcagt
     2401 tacgagtcct caagaatggc tggatgatgc gataaggtgt ttctctggtt tcatcaggca
     2461 agttttctgc ttcaccttca tggtgtttta cttccaccat atacatcagt ccacgcggat
     2521 tgagtggaac ggttgcagtc cacgcatgat caacctgtcg catgggtacg ctctgccagg
     2581 agagttcgct gggcaggggc ttccaccaga gaacgaccgc acttgcggat tgtttactgg
     2641 ataagaagca acgtacggtc gcggtattgc cgctggttgt gctttccaga gaagcagaat
     2701 aaagcccagc ttctttcata tccgaagctt tgggcgtaag aggaagatgt gaagaagagg
     2761 tgttgcggtc catccagaat ttctcggctg tcgcatccag ttccgccaag ggagcgttct
     2821 ggctgctcca tgtgaactcc agttggtggc gcaagggatt ggaaagcggc cggtacacag
     2881 cgtccgttgt ttctcctagc tgcttccatg ccatgcgtga ttgagcaaga ctgcgaaggg
     2941 caatgttgta atcctgtttg ctattggcat gcagggcaaa atcaagatgt gtcatgccgg
     3001 tgatgcggga ggcataatag tcacccagaa gagatgccgc caacaggtcg gtcctcagca
     3061 gtctccactc accggcagcg cgtcctgtgg ggtgtggcat agcatggata agaatgcggg
     3121 tcctggcggc ggaatccgca aagatacggg ccactgcaaa cggacccatg cgtccgtctg
     3181 cacgtccggc caatttttct gcgacgaatt cgtcaatgcc agcaaatacg cgccggtctt
     3241 caggcttgtt ttctgcgaat tgcagaaggt cctggcgcgc gcggcccttc tttgtatcaa
     3301 agttaccagt ctccgtttcc ggagagaagt cgcgctggtc cggtccctgg caacgataag
     3361 cgtaa
//
LOCUS       DASRBV010000085         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_511473, whole genome shotgun sequence.
ACCESSION   DASRBV010000085 DASRBV010000000
VERSION     DASRBV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511473"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1401)
                     /locus_tag="VFA02_03500"
     CDS             complement(19..1401)
                     /locus_tag="VFA02_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU08942.1"
                     /translation="MKKLLLLGPLIFLTACMVGPKYQRPPVSVPQVYRAQMTAATAAS
                     LGNEKWWQVYQDPILVQLIHTALSRNYDLRIAATRVIESQSALGITRANQFPFANVGA
                     GIFSQRNAKITKIFPAYKINAGRVDLSVIWDLDFWGKYRRETQAARADLLATEWGQRA
                     VVSSLVSSVATAYFQLRALDEELEIARRTLSARQESLRLILVLEKNGSASQLDVSQAQ
                     ELVDAAAEAIPDLERQIQQQENLIRNLLGEDPGPVPRGRPLTEQPAPARVPAGLPSEL
                     LERRPDIRMAEANLVAANARIGVVKAALFPDISLTGTGGFESFALNKLISTSGEMWNG
                     AVNLTQPVFEAGALRAGVRLARAQWQEMVLTYQQTIQQAFVQVSNALIACQKDREYRR
                     QQQKLARAAEEADRLSMVLYRNGGASYLQVLTSETNYFAAELNLVQAQLNERLALVEL
                     YQALGGGWQQ"
     gene            complement(1402..>2305)
                     /locus_tag="VFA02_03505"
     CDS             complement(1402..>2305)
                     /locus_tag="VFA02_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU08943.1"
                     /translation="QVYIEGQPAFRGKTDDIKRFYVRNYQGQMVPLSALVQFHPELGP
                     EFVMHYNGFNAAQINGGAAPGYSSAQAMDALEQVFHQTMPASMGFDYMGMSFQEKQAE
                     KGVSATTIFALSLLFVFLILAALYESWTLPFSVLLSTPVAVCGAFGMLLLRRLFVQKF
                     YPVYMVHIDNDVFSQIGMVMLIGLAAKNAILIVEFAKKQYEQGKSLAEAALGGARLRL
                     RPILMTSFAFILGCMPLWFASGTGSVARQIMGTTVIGGMLAASAFGVFFIPVIFYVVE
                     RLSGARKGEAAGQLATEEASDGIE"
BASE COUNT          477 a          699 c          650 g          479 t
ORIGIN      
        1 gacgttgcga gtcggtgctc actgctgcca gccgccgccg agcgcctgat aaagttcaac
       61 cagtgcgagc cgttcattga gttgcgcctg aacgaggttc aattccgccg caaagtagtt
      121 cgtttcactc gtcaggacct gcaggtagct ggccccgcca ttccgataga gcaccatcga
      181 gagacgatca gcctcctccg ctgcccgggc gagtttttgc tgctggcggc ggtattcgcg
      241 gtccttctgg caggcgatga gcgcattcga gacctgcaca aaggcctgct ggattgtttg
      301 ctggtacgtc agcaccatct cttgccactg ggcccgggcc agtcgcacgc cggcgcggag
      361 tgctccggct tcgaaaaccg gctgagtcag gttcacagcg ccattccaca tttctcctga
      421 tgtgctgata agcttgttaa gcgcgaagct ctcaaatccg ccggttcccg tgagtgaaat
      481 gtcagggaaa agggctgctt ttacaacgcc gatccgggcg tttgccgcta ccagattcgc
      541 ttccgccatg cgaatgtccg ggcggcgctc aagcaattcc gaaggtaacc ccgctgggac
      601 tcttgcggga gccggttgtt cagtaagcgg tcggcctcgc ggaacaggac ccgggtcttc
      661 gccgagcaga ttgcggatca ggttctcctg ctgctggatt tggcgttcca ggtcggggat
      721 cgcctcggcg gctgcatcga caagctcttg agcctggctc acatccagtt gcgaagctga
      781 accgtttttc tcgagcacaa gaataagccg aagtgattcc tggcgcgcgg aaagcgtgcg
      841 tcttgctatt tccagttctt catccagggc gcgaagctga aagtaggctg tcgcgacact
      901 cgagaccagc gacgaaacca cggcgcgctg tccccactcg gtggccagaa ggtcggcacg
      961 cgccgcctga gtttcccgcc ggtatttacc ccagaaatcc aggtcccaga tcacggaaag
     1021 gtccacgcgc cccgcgttga tcttataggc tggaaatatc tttgtgatct tcgcattccg
     1081 ctggctgaag atccccgcgc cgacgtttgc gaacggaaac tgattagcac gcgtgatgcc
     1141 aagcgctgac tgggactcga tcacccgcgt ggcggcaatg cgcaggtcgt aattgcggct
     1201 gagcgcggtg tggatcagtt gcaccaggat tggatcctga tatacctgcc accatttttc
     1261 atttcccagc gatgcggctg ttgccgctgt catttgcgca cgatagacct gcggcacact
     1321 gacaggcggt cgctggtact tcggcccgac catgcaggcg gtcaggaaga tgagcggtcc
     1381 gaggagtaag agctttttca tttattcaat gccgtccgat gcttcctcag ttgccagttg
     1441 gcctgccgcc tcgcccttcc tggcgcccga cagccgctct accacataga agatcacagg
     1501 aataaagaag acgccgaagg cgcttgccgc gagcattccg ccgatcacgg tcgttcccat
     1561 gatctggcgc gccaccgaac ccgttcccga ggcgaaccaa agcggcatgc agcccaggat
     1621 gaaagcgaat gaagtcatca ggatcggccg cagccgcaat cgcgctccac cgagcgccgc
     1681 ttcagccagc gatttaccct gctcatactg cttctttgcg aattcgacaa tcagaattgc
     1741 atttttcgcc gccagcccga tcagcatgac cataccaatc tgggaaaaga catcattatc
     1801 gatgtgcacc atgtagaccg ggtagaattt ctgtacgaag agccgccgca acagcagcat
     1861 gccgaaggcg ccgcaaaccg caacaggcgt gctcagcagc acgctgaagg gcagcgtcca
     1921 gctttcgtac agcgctgcga ggatcaggaa aacaaagagc agtgacaacg cgaaaatcgt
     1981 ggttgcggac acacccttct ccgcctgctt ctcctggaat gacattccca tatagtcgaa
     2041 gcccatgctg gccggcatcg tctggtggaa cacctgctca agcgcgtcca tcgcctgcgc
     2101 tgagctgtag ccgggagcag cgccgccgtt aatctgtgca gcattaaaac cgttgtaatg
     2161 catgacaaat tccggcccca gttccggatg gaactgaact agcgccgaga gcggcaccat
     2221 ttgcccctgg taattccgca cgtagaagcg cttgatgtca tcggtctttc cgcgaaaggc
     2281 cggctgaccc tcgatatata cctgc
//
LOCUS       DASRBV010000086         7400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_511527, whole genome shotgun sequence.
ACCESSION   DASRBV010000086 DASRBV010000000
VERSION     DASRBV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511527"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFA02_03510"
     CDS             complement(<1..479)
                     /locus_tag="VFA02_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU08944.1"
                     /translation="MAKTKGYAASAAKAPLAPFTFERREPGEHDVVIDIRYCGICHSD
                     IHQARDEWGGATFPMVPGHEIAGVVSAVGPKVTKYKVGDKVGVGCFVDSCRKCDECKA
                     GLEQYCSVLTSWTYNGIEQDGKTPTYGGYSERIVVDENYALRLPENLPLDASAPLLCA
                     "
     gene            complement(782..1465)
                     /locus_tag="VFA02_03515"
     CDS             complement(782..1465)
                     /locus_tag="VFA02_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZU08945.1"
                     /translation="MSTCGSIRSQFSEYLDGALPGVAMQKVARHLESCGKCSVEFDSW
                     RNTQRMLANLGPAKAPADLALKLRVAISREKAHTPKQSLVRWQMRWQNTIAPFLLQAS
                     AGFASAVLLVGTVAFLVGTVAAPVQASARDDEPLGNASAPRFLYSVPAATPAVLGERE
                     NPVIVEAFIDGSGRVYDYRILSGPMDPHTRTQVENQLLFSVFEPARFFGQPVPGTVIL
                     SFAGVSVQG"
     gene            complement(1462..2163)
                     /locus_tag="VFA02_03520"
     CDS             complement(1462..2163)
                     /locus_tag="VFA02_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU08946.1"
                     /translation="MQAGTAVGNLAGVIAVRPEEAEIVAELKAGSEEAFAWLISTYHQ
                     PIYSVIARTIQNPADAADVTQDVFIKVFRNIAGFHGESSLRTWLYRIALHEASNRRRW
                     WSRHCRQEVTIEAETGQSTEGQALCIKDTLVDEHESPFEMAAHEEVRTHVEAALREVS
                     EPFRTVLVLRDIEGFAYEEIAEILNMNIGTVKSRLLRGRAQLKARLAPFAEAAAKHAG
                     GAARLSAVACPKEAS"
     gene            2394..2597
                     /locus_tag="VFA02_03525"
     CDS             2394..2597
                     /locus_tag="VFA02_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZU08947.1"
                     /translation="MRTNIEIDDQLIEQAMRQSGLRTKKATVEAALRLLVKTRAQAAI
                     RQLRGKVQWEGDLNQSRLGRVKE"
     gene            2603..3004
                     /locus_tag="VFA02_03530"
     CDS             2603..3004
                     /locus_tag="VFA02_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HZU08948.1"
                     /translation="MPVTIVDSTVWIDYFRGESNPHVAWLDRELGRQPLGLTDLILCE
                     VLQGIRDDATFRRVRHHLSFFEIFETGGEKLAIVAAWHYRSLRTQGITVRKTIDCLIA
                     AFCLEYGHSLLHRDRDFDAFENHMGLQVVHP"
     gene            complement(3005..3586)
                     /locus_tag="VFA02_03535"
     CDS             complement(3005..3586)
                     /locus_tag="VFA02_03535"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZU08949.1"
                     /translation="MHPGRIEVITGPMFSGKSEELIRRIRRAKIARQRVLCFKPDIDL
                     RYHKTAIASHSAQTQDACVVPNVERLREALFPQMDEMEVLGVDEAQFFDDGLVSLALE
                     LVHLGKRVIIAGLDTTYAGEPFRPIPDLMAVADEVTKLSAVCMVCGAPAIHTQRLGGS
                     RELVVVGAAGLYEARCRAHFEPFVGSEQLELPT"
     gene            complement(3598..4425)
                     /gene="kdsA"
                     /locus_tag="VFA02_03540"
     CDS             complement(3598..4425)
                     /gene="kdsA"
                     /locus_tag="VFA02_03540"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZU08950.1"
                     /translation="MSHEFEVGTVHVGSGRLFLIAGPCVIESEAHVRRMADAIQRITS
                     DLGVPYIFKASYDKANRTSVTSFRGPGLVEGTAILRRLAEATGLPVLTDVHEPAHCEI
                     AAEGADVLQVPAFLCRQTDLLLSAGRTGRAVNVKKGQFVAPWDMKHAVEKIATTGNTR
                     VFLTERGASFGYNNLVVDMRSLPIMRQFAPVVFDGTHSVQTPSAANGVSGGQPEFIPV
                     LARAAVAAGVDGVFLEVHDNPAEAKSDGANALDLKYLPQVLEALLAIHEAVQAKVVR"
     gene            complement(4635..5885)
                     /locus_tag="VFA02_03545"
     CDS             complement(4635..5885)
                     /locus_tag="VFA02_03545"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZU08951.1"
                     /translation="MKLRKSGRVLLAAAASLGTGLVLTSCSPSNTVDYVYVAASKNNP
                     GQISVYRADSLSGALTQIQESPYPSGGRNPVAMVTSPNGKYLYVVNKDDNNIVQFAIG
                     TDAKLYPQQTCNTPGSAPVAIAINNTGTLLYVVDFYSPTTPGGPVYSSSNPGPGDLVV
                     YPLNSTQALTGCTPVTQTFVDSKGNTQTATYVQLGVNPTGVSSLANSGFVYVSDQNTT
                     SGLGQVEAFSVNSSGTLSGVSGSPFQSGTSPSSLIAVTSQNGTYLYVTDSRQNQLIPF
                     TILSSGALSPNPNGPVKTDTFPFNVTVDPRGQYLYVANYNANDVSAYAIGSNGTPSAV
                     SGASSYATGAGPSCVLVEPALGRFVYTANFIDNTVTGFELNPNTGVLTATQNSPYQAA
                     GQPTCAAAVPHGNHALQNVQSTAR"
     gene            complement(6021..7379)
                     /locus_tag="VFA02_03550"
     CDS             complement(6021..7379)
                     /locus_tag="VFA02_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08952.1"
                     /translation="MKFRKFGQMALAAVVTAVLVGGTVSCRNDYTTAFLFTVGSQYNQ
                     LASYRVSYETGGLSAAPGSPYGSGGTNPVRVLVLSGGRYIYVLNNGAQQTDSSGNISY
                     TGANISVFNVGGYGNLAFQTSYSSQGSDTIRILTDTSGKYLFALDRYVPVLTSGGTFT
                     STFAPQNNYSSTYPCQGPDGKYYPTGGITVFAIDSNTGRLSIVTNQQQQASTANGSVQ
                     LTYFPVGCFPVDFTLSGGYVLTADAGSSTNNDVQTVFPYQLNASNGQLTLTQNAPLAM
                     GTNSMTAISTAKASTLTGASAVYVLDGGTNQIRSYTVGSNGILQLQSSGVTQNASTAS
                     NPTALIVDSQGKYLYVANSGPTSGTVNPISQITGYVIVDNTSGALSPIAGEPYGSGSG
                     VSCILEDTSNQYIYTTSFNDSQITGRILDPKSGSLTLLRRGSTFNTVGNPTWCAISGR
                     TS"
BASE COUNT         1613 a         2189 c         2013 g         1585 t
ORIGIN      
        1 gcgcaaagca gcggtgccga tgcatcgagt ggaaggttct ccggcaggcg cagagcatag
       61 ttctcatcca cgacaatccg ctccgaatag ccgccatagg tcggcgtctt gccgtcctgt
      121 tcgatgccgt tgtaggtcca ggacgtgagc acagagcagt attgttcgag tcccgccttg
      181 cattcatcgc atttgcggca ggagtctaca aagcaaccca cgccaacttt atcgcctacc
      241 ttgtatttcg tgacctttgg gccgacggcg ctcacaactc cggcaatttc atgcccgggc
      301 accatcggaa atgtggcccc accccattca tcgcgcgcct gatgaatatc agagtggcag
      361 atgccgcaat atctgatatc aatcacaacg tcatgctcac ccggttcccg gcgctcgaat
      421 gtaaatggcg caagcggggc cttcgccgca gatgcggcat aacctttggt ttttgccatg
      481 gaagttcctt tccaggaaag agtttactgg tttagatgta tcagcaaggt aagtggattc
      541 cgacccagtg ttgccattca tcacaaagcc actttggcga ctttatagca ttttcacaga
      601 tagtctggac catgccccga aactacagtc gccgcgacca acagggagtc ggcgacactg
      661 gacagtgcaa atgggagtgt acacaatctg tgaaaatgcc ttagataagg ccgtccgcca
      721 tctcgccggc aatgaatatg ccagacacgt ccatgtcatc gagtaaccgg gaagatagaa
      781 cttatccctg caccgacacg ccggcgaacg acagaatcac cgttccagga accggctgac
      841 cgaagaaacg cgcaggctca aaaacagaaa acagcaattg gttttccacc tgcgtccgcg
      901 tgtgcggatc cataggcccg gaaaggattc ggtaatcgta gacacgcccg cttccgtcaa
      961 taaacgcctc gacgatgaca ggattctccc gttctcccag taccgccgga gttgccgcag
     1021 ggaccgaata caggaaccgt ggggctgaag cattccccag cggctcatca tcacgtgccg
     1081 aagcctgtac cggggccgcg actgtcccca ccagaaatgc caccgtccct acgagcaaaa
     1141 ccgcgctcgc gaacccggca gaggcctgca gcaggaatgg cgcgattgtg ttttgccagc
     1201 gcatctgcca gcgcacaaga ctctgctttg gagtatgggc cttctcccgg gaaatggcca
     1261 cacgcagctt cagggcaaga tcggcaggtg ctttcgccgg gcctagattg gccagcattc
     1321 gctgcgtatt gcgccagctg tcaaattcca ctgagcactt cccgcaggac tccagatgcc
     1381 tggcgacctt ctgcatggcc actccgggaa gagcgccatc cagatattcc gaaaactgcg
     1441 aacgaatgct gccgcacgtg ctcatgatgc ctcctttggg caggcaacag cagaaagcct
     1501 ggccgcgcct ccggcatgtt tggccgcggc ttcggcaaac ggggcaagac gcgccttcaa
     1561 ctgcgctcgt ccgcgcagaa ggcgtgattt tacagtgccg atattcatgt tcagaatctc
     1621 ggcaatctct tcgtaggcaa acccttcgat gtctctcaga acaagcaccg tccggaacgg
     1681 ctcggaaact tcgcgcagag cggcttccac atgggtgcgg acctcctcat gcgcggccat
     1741 ctcaaaggga gattcgtgct catctaccag agtgtctttg atgcagagcg cctgcccttc
     1801 cgtagactgt ccggtttcag cttcgatcgt cacttcctgg cggcaatggc gcgaccacca
     1861 gcggcggcgg ttggatgcct cgtgcagggc gatgcgatag agccaggtac gcaggctgga
     1921 ctcgccgtga aatccggcaa tgttgcggaa gactttgatg aacacgtcct gcgtgacgtc
     1981 tgctgcgtca gcggggttct ggatggtgcg cgcaatgaca ctgtagatag gctgatgata
     2041 ggtagagatc agccatgcaa atgcctcttc agagccggcc ttcaactccg cgacaatttc
     2101 ggcctcttcg ggccggactg caatcacgcc cgccagattt cccactgctg ttcctgcttg
     2161 cacccagttt ctccttatac agtaacaaac cggagaggtg tctggcatct ccgccttgcg
     2221 ggcggcctgt cctctgttcc tgtaaccgcc ggcggccgag tgtctcgctc actctacaga
     2281 cttagacacc ggaacagcca acagaggttc ccggaaaaga ggaaagaaat gatggtctca
     2341 gtcctctcga taactccgga tacacataaa cgtgctattc ttacacataa accatgagaa
     2401 caaatatcga gattgacgac cagttgatcg aacaggccat gcggcagagc ggtttgcgga
     2461 ccaagaaggc cactgtcgaa gctgccctgc gtttacttgt gaagacccgc gcacaggcag
     2521 caattcgtca gcttcgcgga aaagtacagt gggaaggtga tttgaatcag tcccgcctgg
     2581 gccgcgtgaa ggaataagtc ccttgcctgt aaccatcgtg gattccactg tctggattga
     2641 ttattttcgg ggagagtcca acccgcatgt tgcctggttg gaccgggaac taggccgaca
     2701 gcccctggga ttgactgacc ttatcttgtg tgaggtcctg caagggatac gcgatgacgc
     2761 cacgttcagg cgcgtcagac atcatctctc cttctttgag atatttgaaa ccggcggcga
     2821 aaagctggct atagttgctg cctggcatta ccgctcactt cgcacccagg gcattacggt
     2881 acggaaaact atagattgcc tgattgccgc attctgcctt gaatatggtc actcgctgct
     2941 tcaccgtgac cgcgattttg acgcgtttga aaaccatatg ggattacagg tggtccaccc
     3001 ataactatgt cggcagttcc aactgctccg accccacaaa tggttcaaaa tgcgctctgc
     3061 atcgcgcctc gtataatcct gcggccccca ccacaaccag ttcccggctt cctcccagcc
     3121 gttgtgtgtg gatggcaggc gcgccacaca ccatgcacac agccgagagc ttcgtcacct
     3181 catccgccac tgccatcagg tcaggaatcg gtctgaaagg ctcgcccgcg taagttgtgt
     3241 ccagcccggc aataatgacg cgcttgccca gatgcaccag ctcgagagca agcgaaacca
     3301 gaccgtcatc aaaaaattgc gcctcgtcta ctccaagcac ctccatctca tccatttggg
     3361 gaaagagggc ttcacgcaga cgctccacgt tgggaacaac acaggcatcc tgcgtctgcg
     3421 cgctatgcga agcaatggcc gtcttgtggt aacgaaggtc tatgtccggc ttgaagcaga
     3481 gcacgcgctg ccgggcaatt ttcgcccgcc ggatgcgccg gatcaactcc tcggacttgc
     3541 cggaaaacat cgggccggta atgacctcaa tgcggcctgg atgcatctcc tgggtcacta
     3601 ccgcaccacc ttcgcctgga cggcctcatg gatcgcaagc agggcctcca gcacctgcgg
     3661 caggtatttc aggtcgagcg cattggcccc gtccgacttc gcctctgccg gattatcatg
     3721 cacctccagg aatactccgt ctactccggc agccaccgcc gcgcgcgcca gcactggaat
     3781 aaactccggc tgccctccgg aaacaccatt ggccgctgag ggcgtctgca ccgaatgcgt
     3841 gccgtcaaac accactggag caaactgtcg catgatgggc agcgagcgca tgtccaccac
     3901 aagattgttg tagccaaagc tggccccgcg ttcggtcaga aagacgcgcg tatttcccgt
     3961 tgtagcaatc ttttcaaccg cgtgtttcat gtcccacgga gctacaaact gccccttttt
     4021 cacgttgact gcccgtcctg tgcgcccggc ggaaagcagc aggtcggtct ggcggcacag
     4081 aaaagccggg acctgtaata catccgcccc ttctgccgca atctcgcagt gcgccggttc
     4141 atgaacgtcc gtcagaacag gaagcccggt cgcttcggcc agacgccgca ggatggccgt
     4201 gccttccacc aggccgggcc cgcgaaagct ggtcaccgag gtacgattgg ccttgtcata
     4261 cgaagcctta aagatatacg gaacgcctaa gtccgatgta atacgctgaa tggcatcggc
     4321 catcctgcgt acgtgcgctt cactttcgat gacgcacggg ccggcgataa gaaacagtct
     4381 tccagagcca acgtgcacag tcccgacttc aaattcatga ctcacgtatt tgattgtaaa
     4441 gggggaccgc gcgtttacgg tgggcttgcc cgggcatggt cttgcgggaa gatgctctcc
     4501 tcaggaagga aagaggtaga gtccatccac ttcagggcac tgtcggatca acacccctta
     4561 tacagaaaat cagtttcaga aagtaaaaag gccagctcgt gagagcgggc cttcttacat
     4621 ggattcagct tggattacct ggccgtgctc tgcacattct ggagagcatg gtttccgtgc
     4681 ggaaccgcgg ctgcgcacgt cggctgtcct gcggcctggt acggagagtt ctgggttgcc
     4741 gtcagcactc cggtattcgg attcagctca aatccggtca cagtgttgtc aataaagttc
     4801 gcggtgtaga cgaagcgtcc caacgccggc tcaaccagta cgcacgaggg ccctgctcca
     4861 gtcgcgtagg aactggctcc cgatacggcc gaaggcgttc cattcgagcc gatcgcatag
     4921 gcgcttacgt cgttcgcatt gtagttggca acgtagagat attgcccgcg cgggtctact
     4981 gtgacattga acgggaaggt gtctgtcttg accggcccat tcgggttcgg actcagcgct
     5041 cccgaggaaa gaatcgtgaa cggaatcagt tggttctggc ggctgtccgt cacatacaga
     5101 tacgtgccgt tctggcttgt caccgcaatc agcgaagaag gcgacgtgcc ggactggaac
     5161 ggacttccgg acacacctga cagcgtaccg ctggaattta cgctgaatgc ctcaacctga
     5221 ccaagacctg acgtcgtatt ttggtcagac acgtagacaa atcctgaatt tgcaaggctc
     5281 gaaacacccg tcgggttcac acccaactgc acataggtcg ctgtctgcgt attgcctttg
     5341 gaatccacaa atgtctgcgt gaccggagtg cagccagtca gcgcctgcgt ggaattcagc
     5401 gggtaaacaa ccagatctcc ggggccggga ttcgagctgc tgtatacagg gcctcccggt
     5461 gtcgtcggcg agtagaagtc cacgacataa aggagcgtgc ccgtgttatt gatggcaatc
     5521 gccaccggcg ccgaccctgg cgtattgcag gtctgctgcg gataaagctt cgcgtccgtg
     5581 ccaatcgcga attggacaat gttgttgtcg tccttattca caacgtaaag atatttgcca
     5641 ttcggcgatg tcaccatggc cacggggttc ctgccgcccg aaggatacgg cgactcctgg
     5701 atctgcgtca gagcgccgga cagtgaatcg gcccggtata cactgatctg cccagggttg
     5761 tttttagagg ctgccacgta aacgtagtca accgtgttgc tggggctgca ggaggtaagc
     5821 acgagacctg ttcccagtga tgctgctgcg gccagaagca cccggcccga tttcctcaac
     5881 ttcatgcgct tccttcatcc tggcctcaag tggcgcaggt tttttctttt ctgataagga
     5941 ggattgctcc ccctgcattg taaagtcggg ggccgcattc ggccatgcgt ctgcggcccg
     6001 tactgcctca aaaatacccg tcagcttgtg cgtccgctga tcgcgcacca ggttgggttc
     6061 cccactgtat taaatgttga accgcggcgc aacagcgtca gactgccgga tttcgggtcg
     6121 agaatgcgcc ccgtaatctg gctgtcatta aagctcgtgg tgtaaatgta ctggttcgac
     6181 gtgtcctcca gaatgcagct cacgcccgag cccgacccat acggctcgcc agcaatagga
     6241 gaaagcgcgc cgcttgtatt gtccacaatc acatagcctg tgatctggct gatcgggttg
     6301 actgtgccgc tcgtcgggcc ggaattcgcc acatacagat acttgccctg gctgtccaca
     6361 atcagcgccg tcggattgga ggccgtcgaa gcattctgcg ttacgccgct cgattgcaac
     6421 tggaggattc cgttcgagcc aaccgtgtac gaccggatct ggttggtccc gccatccagc
     6481 acgtacacag ccgatgcccc ggtcagcgta gaggctttgg ccgtgctgat ggcagtcata
     6541 ctgttggtcc ccatcgccag cggagcattc tgcgtaagcg tcaactgccc attggaagca
     6601 ttcaactgat aaggaaagac cgtctgcaca tcattattgg tggagcttcc cgcatctgcc
     6661 gtcagcacat acccgcctga aagcgtaaag tccaccggga agcagcctac aggaaaatac
     6721 gtaagctgta ccgacccgtt ggcggtgctc gcctgctgct gctggttcgt cacaatcgaa
     6781 aggcgacctg tattgctgtc aatcgcgaag accgtaatgc cgcccgtcgg ataatacttt
     6841 ccatccggcc cctggcatgg atacgtactg ctgtaattgt tctgcggagc aaacgtgctg
     6901 gtgaaagtcc cgcccgaagt cagcacggga acatacctgt ccagtgcaaa cagatattta
     6961 ccgctcgtgt ccgtcaggat gcggatcgtg tccgaaccct ggctcgaata gcttgtctgg
     7021 aaggcaaggt tgccgtaacc acccacgttg aaaacggaga tgttcgcgcc ggtgtagctg
     7081 atatttccgc tcgagtcggt ttgctgtgct ccgttgttca gcacataaat gtagcgcccg
     7141 ccgctgagca caagcacgcg aacaggattt gtgccgccag aaccatatgg cgacccggga
     7201 gcagcactca gccctccggt ttcatagctg actctgtatg aagcaagctg gttgtattgc
     7261 gatccgactg tgaagaggaa tgccgtcgtg taatcgttcc ggcacgaaac agtcccgcca
     7321 accaggactg cggtaactac cgcggccaat gccatctggc caaattttct aaacttcatg
     7381 cgcttccttc atcctggcca
//
LOCUS       DASRBV010000087         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_156239, whole genome shotgun sequence.
ACCESSION   DASRBV010000087 DASRBV010000000
VERSION     DASRBV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156239"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VFA02_03555"
     CDS             complement(<1..1486)
                     /locus_tag="VFA02_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZU08953.1"
                     /translation="MAYNDLRDWIKTLEKSGELKRVREEVDPVLEIAEITDRASKSPN
                     GGPALLFENVKGHPGIPVLMNQFGSEHRMKLALEVDSLDEIAGRIRTLLDVKSPEGLL
                     EKLKMLPMLADIGRFFPKTISAKDAPCKEVILHENFNVLHFPVLKCWPLDGGRFITLP
                     CVITRDPKTGKRNVGMYRMQVYDGQTTGMHWQRQKNAAEHLRERLRVAAGESAAAHVH
                     VMAETAGGTTNITLTNQKEARLEVAVAIGTDPATTFSAIVPAPPEVEEFMIAGFLRQK
                     PVELVKCETVDLEVPAHSEIVLEGYVKLDELRTEGPFGDHTGFYTMEDEYPVFHITCI
                     THRKNPIYASTIVGKPPMEDAWMGKAVERIFLPLMRLTLPEIVDVNLPVEAVFHNLMV
                     VSIRKSYAGQARKVMNGIWAMGQAMFTKCIIVVDEDCDVQDLREVTLRVANNIDPERD
                     IQFTLGPIDSLDHASRLPNYGSKMGIDATRKWAAEGFTRPWPPMI"
     gene            1777..2571
                     /locus_tag="VFA02_03560"
     CDS             1777..2571
                     /locus_tag="VFA02_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08954.1"
                     /translation="MTYSHALEEPDNLEVAFKGTQGAPKYGNSFAGGTLELEYGARAW
                     WTTEVYLSGQTTANDSTVFTGFRWENRFRPLLEEHWINPVFYVEYEDHNTADRSLLEV
                     VGHDSVSDLRIGNAEARKDVEHEMEMKLILSSNAKGWNFSENFISEKNVTQSEPWEFG
                     YALGASRPLSLKATARKCVFCIENFAAGAELYGGLGDSDGFGLKQTSHYFGPNIQWNL
                     PNGPAITFSPNIGLNDNSLGVLYRVGVSYEVQQLFGRFHRGQGGLR"
     gene            2568..2981
                     /locus_tag="VFA02_03565"
     CDS             2568..2981
                     /locus_tag="VFA02_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU08955.1"
                     /translation="MSRRRLSGFRAQLLWMALVLCAAAAFAQDAKGWPQRVPEKDRQR
                     VDPYSGHADAIAAGAKLYAQHCSKCHGENLEGSGNKPSLHSEAVQQATDGELFWMLKN
                     GDLRHGMPSWSSLPEPARWQIIAFIRSAKTPMNEP"
     gene            3049..>4394
                     /locus_tag="VFA02_03570"
     CDS             3049..>4394
                     /locus_tag="VFA02_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08956.1"
                     /translation="MMCLLSAGIVSAGFVIGCGSGLAPNFPSENDSTPAADSIGEALF
                     LDTRFSEFFAAHMTGVNDPLPAGDPVVQQVQTTNGTLPGPFAGQSINCRSCHFVTEFQ
                     GVAGAGNRTYADFTTRSPIPRPLNGFDHTQRNAMQMVGSLQTHSGSTFLHFDGEFASP
                     EDLVEATITGRNFGWAPAQFQQAVAHIARVVREDDGLGQLAADRLNGLSYAVIFKGTD
                     PRIPSDLRLPESQRLDVQTATDQQILDEIALCISTYMKDLLFKQDEFGRYIASPYDVF
                     LRINHLPVQPRAGQSSAAYVQELLSALNSLSSPLWVDSSYGAFLYHNQKFQFGPQELA
                     GLKIFLRSAVNAADGSQHAGNCAACHLPPDFTDFQFHNTGVTQEEYDSVHGSGAFMKL
                     YIPPLSVRNQNYDQYLPVTTEHPNAQEPFRRAASAEQAAYADLGLWNIYLNPDRPKP"
BASE COUNT          925 a         1237 c         1242 g          990 t
ORIGIN      
        1 caatcattgg cggccatgga cgggtaaatc cttccgccgc ccacttgcgt gtggcgtcaa
       61 tgcccatctt gctgccgtag tttggcaaac gcgaggcatg gtcgagcgaa tctatggggc
      121 caagcgtgaa ctggatatcg cgttcagggt caatgttatt ggcgacgcgt agcgtgactt
      181 cgcgcagatc ctgcacgtcg cagtcttcat ctacgacaat gatgcacttg gtgaacatcg
      241 cctggcccat ggcccagatg ccgttcatca ccttgcgcgc ctgccctgca tagctcttgc
      301 ggatggagac aaccatcagg ttatggaaga cggcctcgac cggcaggttc acatctacga
      361 tctccggcag cgtaaggcgc atcagcggaa gaaagatgcg ttcgacggcc ttgcccatcc
      421 atgcgtcctc catcggaggc ttacccacaa tggtggaagc gtagatgggg tttttgcggt
      481 gcgtgatgca ggtgatgtga aagacagggt actcgtcctc catggtgtag aaaccggtgt
      541 ggtcgccgaa agggccttca gtgcgcagct catcgagctt gacgtaacct tcaaggacga
      601 tctcagaatg tgcgggaact tcaaggtcca cggtctcgca cttcaccagt tctaccggct
      661 tctgccgcag aaagcccgca atcatgaatt cttccacttc cggcggagcg gggacgatgg
      721 cagaaaacgt cgtcgcagga tccgtcccga tggcaactgc tacttccagg cgggcttctt
      781 tctggttcgt cagcgtgatg tttgtcgtac cgcctgctgt ttcagccatt acgtggacgt
      841 gggccgcagc ggattctccg gcggcgacgc gcaaccgttc gcgcaggtgc tcggcggcgt
      901 tcttctgcct ctgccagtgc atgcctgtcg tctgtccgtc gtacacctgc atccggtaca
      961 tgcccacatt gcgttttcca gtcttcgggt cgcgcgtgat aacacagggc agcgtgataa
     1021 aacggccccc gtcgagcggc cagcacttca gaacagggaa gtggagcacg ttgaagttct
     1081 cgtgcagaat gacttccttg cagggggcat cttttgcgga gatggtcttc gggaagaacc
     1141 tgccgatgtc ggccagcatg ggcagcatct tcagcttttc cagcagacct tcgggcgatt
     1201 tcacatccag cagggtgcgg attcgtccgg caatctcgtc gagtgaatcc acttcgagcg
     1261 ccagcttcat acggtgctca ctgccgaact ggttcatcag gacggggatg cccgggtgtc
     1321 ccttcacgtt ttcaaagagc agcgccgggc ccccgttcgg cgatttcgag gcacggtcag
     1381 tgatttcagc gatctcgaga acaggatcca cttcttcgcg gacacgcttc aactcgcctg
     1441 atttttcaag ggttttgatc cagtcgcgca ggtcgttgta ggccacaatg cctccagaat
     1501 caggtgcaaa agttcatgtt acctgaatca aaggccgtga ttttttatgc tcttttttgt
     1561 tgacgaataa atgcgaccga tttagtctgc aataaataag actgattcag tcttaattat
     1621 gacggcatgg caaagcctgc cgtaaatcct ttattttgag gctttccttt gtttctgttt
     1681 gcacggacct gtcttttgcg gctgtcgtgg ggtgctcttt tcgtcagcct tcttttcttg
     1741 tgccagaatg cgcaggcgca ggaacctcct tactttgtga cctactcgca cgctctggaa
     1801 gagcccgaca acctggaggt cgcctttaag ggaacccagg gtgcgccaaa gtacggcaac
     1861 tcgtttgccg gtgggactct ggagctcgaa tacggcgcgc gggcctggtg gacgacggag
     1921 gtctatctct ccggccagac gaccgcgaac gacagcacgg tctttaccgg gttccgctgg
     1981 gaaaaccgct tccgtccgtt gctggaagag cactggatca atcctgtttt ctatgtggag
     2041 tacgaagacc ataacacggc agaccgcagt ctgctggaag tggtcgggca tgacagtgtc
     2101 tctgacctgc gtatcggcaa tgcagaggcg cgcaaagacg ttgagcacga gatggagatg
     2161 aaactcatcc tttccagcaa cgcgaaagga tggaatttct cggaaaactt catctcagaa
     2221 aaaaacgtga cccagagcga accatgggaa tttggctatg cgctgggcgc cagccgtccg
     2281 ctctcgctga aggccactgc cagaaagtgc gtattctgca ttgagaactt cgctgcaggg
     2341 gccgagcttt atggcggcct cggcgatagt gacggcttcg ggttgaaaca gacttcgcat
     2401 tacttcgggc cgaacatcca gtggaacctg cctaatgggc ctgccattac cttctccccc
     2461 aatatcggcc tgaatgacaa cagcctcggg gtcttgtacc gcgtgggcgt gtcgtatgaa
     2521 gtccagcagt tgtttggacg gttccaccgc gggcagggag gtttgcgatg agcagaagac
     2581 ggttatcagg cttcagggcg cagctccttt ggatggctct ggtcctttgc gctgctgccg
     2641 cattcgcaca agatgcgaaa ggatggccgc agcgtgttcc ggagaaagac cgtcagcgcg
     2701 tagaccctta cagcggtcat gctgatgcga ttgccgccgg ggcaaagctc tatgcgcagc
     2761 actgctccaa atgccacgga gaaaaccttg agggcagcgg aaacaagccc agcctgcaca
     2821 gcgaagcggt gcagcaggcg acagatggag agttgttctg gatgctgaag aacggtgacc
     2881 tgcggcatgg aatgccctcc tggagcagcc tgcctgagcc agcgcgctgg cagattatcg
     2941 cctttatcag aagcgcgaaa acaccgatga acgagccgta agcgctctgc gcatcggcag
     3001 atggaaattt caaaggacaa gccaggaaag actatgcgca gaaaatggat gatgtgtctc
     3061 ctgtcagcag gcattgtgag tgccggattt gttattggat gtggaagcgg ccttgcgccc
     3121 aattttccct cggaaaatga ttcaacaccc gcagccgatt ccatcggtga ggcactcttt
     3181 ctcgatacac gcttttctga attctttgcc gcgcacatga ccggcgtgaa tgatcctttg
     3241 cccgcgggtg accccgtcgt gcagcaggtg cagacaacca acggaacact cccaggcccc
     3301 ttcgccggac aatccatcaa ctgccgctcc tgccactttg tcacggagtt tcagggtgtc
     3361 gcgggagcag gcaatcgcac atatgccgat ttcaccaccc gcagcccgat tccccggcca
     3421 ctgaatggct ttgatcacac acagcgcaat gccatgcaga tggtcggttc cttgcaaacg
     3481 catagcggct ccacattcct gcattttgat ggggaatttg cttctcctga agaccttgtc
     3541 gaagccacca tcaccgggcg caactttgga tgggcacctg cacagtttca gcaggcagtt
     3601 gctcatatcg cgcgtgtggt tcgggaagac gatggcttag gccagcttgc cgcagacagg
     3661 ctgaacggac tttcctacgc agtgattttc aaaggcacag acccgcgcat tcccagtgac
     3721 ctgcgcctgc cggaatcgca gcgccttgat gtgcagacgg cgaccgacca gcagattctg
     3781 gatgagattg cgctgtgtat ttctacctat atgaaggacc tgctcttcaa gcaggacgag
     3841 ttcggccgtt acatcgcctc tccttacgat gtgtttctgc gcatcaacca tctgcctgtg
     3901 cagccgcgcg cggggcagag ctccgctgcc tatgtccagg aacttctcag cgcgctcaat
     3961 tctctgagca gtccgctgtg ggtggattcc agctacggag cgtttcttta ccacaaccag
     4021 aaatttcaat ttgggccgca ggagcttgca ggactgaaga tatttctcag aagcgcagtc
     4081 aatgctgcgg atggttccca acatgcaggc aactgcgccg cctgccacct gccgccggac
     4141 tttaccgact ttcagtttca caacaccggt gtcacccagg aagaatacga ttctgtgcat
     4201 ggcagcggcg cattcatgaa gctttacatt ccgccgcttt ctgtcagaaa ccagaactac
     4261 gaccagtatc tgccggtgac cacggaacac ccgaatgcgc aggagccgtt ccggagggcg
     4321 gcatcggcgg aacaggcagc ttacgccgat ctgggcctgt ggaacattta tctgaatccg
     4381 gaccggccca aacc
//
LOCUS       DASRBV010000088         6752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_410000, whole genome shotgun sequence.
ACCESSION   DASRBV010000088 DASRBV010000000
VERSION     DASRBV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410000"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1160
                     /locus_tag="VFA02_03575"
     CDS             138..1160
                     /locus_tag="VFA02_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU08957.1"
                     /translation="MNTTSQSMPSITPERISRYFWAFAFPLIMKAATDNGIFDALKDG
                     PLTAEEISIRTQTSPRGTSAIVEALTGMELLEKMDRRYRLANDAAAFLVSDRPGYLGG
                     MLHHLTKQILPDWMQLSEITKTGRPAVGVNQEDYGAAFFEKFVEALFHFNFGAACALG
                     KALAPKYSGRPVRVLDIAAGSGVWGIGIAQALPQAQVTALDFARVLETTKRCVERVHL
                     TDQFTFVPGNALEADPGSGFDIAVLGHILHSEGAERSRQLLKRVFDQLAPGATVAIAE
                     FLVNQERTGPVNSLIFNVNMLVHTDAGAAYSFEDLKQWLEAAGFIHVRTLEAPAPSPL
                     VLAEKQ"
     gene            1175..1402
                     /locus_tag="VFA02_03580"
     CDS             1175..1402
                     /locus_tag="VFA02_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08958.1"
                     /translation="MEIREGLRQACAMLMIALALASGRIVRSYLYGVSARDGWTLASV
                     AAVLFLCGLLAAWPPARRAALVDPMEALRAE"
     gene            1462..4047
                     /locus_tag="VFA02_03585"
     CDS             1462..4047
                     /locus_tag="VFA02_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZU08959.1"
                     /translation="MKIKKASPNLLRFFLGLFILASLGYWISLSLSFWAVAAHPHDHV
                     RDPFRYDRDSRIIKEVQPEARSAGIQEGAVLESLNGVPYTAASWAEILNSARPGDMMD
                     VGFQRKDGSQGEATITLVTLAGDPHLPPLSVFKAQTVVFSIISLACLLMGYWVVMAKP
                     LEANAWLLLVLLSGPSVIVFNGSGLATGVFGFALQFWYVLLQIAMPPALLLFGIYFPE
                     PSRIDARFPWIKWIVLGPLLVCALIGFSAFYGEFYGSGNPAWLAQAATWAGHDQDFLN
                     LLCVLLYLAFTVDKLRTASTEDARRRLRVLTAGMSVSLVAVLVIFIILPHFGITATNS
                     RLLWLTYVGILCLLLAPFTLVYVVLVQRAMDVRILIRMGARYALAKATLWFVQIALIT
                     VLAVELLLPVFGKKQQSPMGVIGVLVFLAIVLVLRSGLQKRAQQWIDRRFFREAYDAE
                     RVLNDLAEEVRRFTETGPLLETVARRIAETLHVSQIAMLLRRGEQFVLQKSIGLSTQN
                     GLVLPVQSSSVRYLTNSNQPARLYRDDPDAWYLMAGTAERYTLDQINAELLLPLPGRN
                     RMMGVMALGPKRSEAAYSSADMKMLQALASQTGLALEVSELARSLAAEAAQRERANRE
                     MEIAREVQERLFPQEMPQLPGASVAGFCRPAQGVGGDYYDVIDMGDGRIGLAIGDVSG
                     KGISAALLMASLRASLRGVTLDGPRDYARLMHKVNRLVYEASASNRYATFFFGSYDPA
                     THMLECVNAGHNPPIVLRGKEVIRLNPDGPVVGLLPSAPYTEQKIQLRAGDALVLYTD
                     GISEAMNKTDEEWGEERMIAAAQTARDKTACALLETIFAAADGFTAGAPQHDDMTLLV
                     LKMDE"
     gene            4346..4627
                     /locus_tag="VFA02_03590"
     CDS             4346..4627
                     /locus_tag="VFA02_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08960.1"
                     /translation="MSTVALKEAELPLAADDFNALEQRILRTVELLKKEREARAAAEQ
                     NAATLQQLLDEQSLELSKTQDHVKALEQEREAARQRVERMLKQLDEISA"
     gene            4658..4996
                     /locus_tag="VFA02_03595"
     CDS             4658..4996
                     /locus_tag="VFA02_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZU08961.1"
                     /translation="MANSQQETKDAAQFVTVDIYDQAYRLRGPDPAYIQKLAEIVDTK
                     MRAVAAQGKTVDSLRVAVLAALNIADEMSALEERYKQLTGSAKETIRNRASRLTGLLD
                     SVLSEERKIS"
     gene            5051..5241
                     /gene="ssrS"
                     /locus_tag="VFA02_03600"
     ncRNA           5051..5241
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFA02_03600"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5259..6065
                     /locus_tag="VFA02_03605"
     CDS             5259..6065
                     /locus_tag="VFA02_03605"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZU08962.1"
                     /translation="MYPRLFQFGHIAIPAYGVFAALAILAALAVSTQTARRLSLNPET
                     VWNLGLTGIFTALIGARLLLALFHPRDFLAHPFWMMGLVSVRSNTAFYGSLVLAICIC
                     CGYIFASRLPLRRTLDCIAPAAALGLGIRALGAFAAGSDYGSPTSAPWGVTYTHRLAS
                     LWSGTPLGTRLHPVQLYEAAVLFALFALLMFLLPRYQHAGDLAALFLFVYGAALYFLD
                     FCRGAHSFVFAEFLSIAQICGILSVLVGLVLFWQTSGRQQNRGPATGKIS"
     gene            complement(6069..>6752)
                     /locus_tag="VFA02_03610"
     CDS             complement(6069..>6752)
                     /locus_tag="VFA02_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU08963.1"
                     /translation="MKELRLTDLNAKDVKDHLPMSPPEATDAALRDYAANGIKLTAVG
                     AVYFPKDEDDDIRTKFEYAKRAGVKVIVAGDPLPRALPRIEKFVKEYDIRFAIHNHGP
                     EDKIWPSPLDILKDIRNMDPRMGCCIDVGHCARTGTDPVEAIRKVGPRVFDIHMKDLA
                     DLRDKASQVAVGDGKMPVRAIFEALIAIRYKGHVDLEYEIHGDDPMPGVTKSFAYMRG
                     VLAGLGYQA"
BASE COUNT         1342 a         2130 c         1860 g         1420 t
ORIGIN      
        1 aacagctcta taacaccaat tcccggatga caatctttca gtgcctgcat ctggacaggc
       61 acttcttttt tcgtgacacc aaagcaagtg cagtagcact atgtgtggtg cgggcccccg
      121 ctgcataaga agtcacgatg aacacaacga gccaatccat gccatccatc acgcccgagc
      181 gcatctcgcg ctatttctgg gcatttgcct ttcccctcat catgaaagct gccaccgaca
      241 atggcatctt cgatgcattg aaagacggac cgctgacagc agaagaaatc tccatccgca
      301 cccagacttc cccccgcggg acctccgcta tcgttgaggc gcttacagga atggagcttc
      361 ttgagaaaat ggaccgccgc taccggcttg ccaacgatgc cgcagccttc ctggtaagcg
      421 acagaccagg ctatctgggc ggaatgctcc atcacctgac aaagcagata ttgcccgact
      481 ggatgcagtt gagcgaaatc acaaagactg gcagaccggc tgtaggcgta aaccaggaag
      541 actacggcgc cgccttcttt gagaagtttg tcgaggcgtt gtttcacttc aactttggag
      601 cagcctgcgc attaggcaaa gcccttgcgc ctaaatattc cggaaggccc gttcgcgtgc
      661 tcgacatcgc cgctggctcc ggcgtctggg gcatcggaat tgcgcaggca ctgccacagg
      721 cgcaggtcac ggcccttgac ttcgcgcgtg tgctcgaaac cacgaagcgc tgcgtcgaac
      781 gcgtccattt gacggaccag ttcaccttcg tcccgggaaa cgcactggag gccgacccgg
      841 gcagcggctt tgatatcgca gtcctcggac acatcctgca cagcgaaggt gcggaacgca
      901 gccgccagtt gctgaagcgc gtctttgacc agcttgctcc cggcgcgact gtcgccattg
      961 ccgaattcct tgtcaaccag gagcgcaccg gaccggtaaa cagcctcatc ttcaacgtca
     1021 acatgctggt ccacaccgac gcaggtgcag cctactcctt tgaagacctg aaacagtggc
     1081 tcgaagccgc cggtttcatc catgtccgca cactggaggc gccggcaccc tctccgctgg
     1141 tgctggcaga aaagcagtag acaatctaca gctcttggaa atccgagagg gcctgcgcca
     1201 ggcctgcgcg atgctcatga tcgctctggc gctcgcctcc ggacgcatcg tgcgcagcta
     1261 tctctacggc gtaagcgcgc gggacggatg gacgctcgca tccgtagccg ccgtcctgtt
     1321 cctctgcgga ctactcgccg catggccccc tgcccgccgt gcagccctgg tagacccgat
     1381 ggaagcgctc cgggccgagt gaaccgtacg aaagtttcat gcgtaaaagc atcaggccgg
     1441 atactatgaa ggccagacct gatgaagatc aaaaaggctt ctccgaacct gctgcgcttt
     1501 ttcctggggc tgtttatcct ggccagtctg gggtactgga ttagcttgtc tttaagcttc
     1561 tgggccgtag cagcgcatcc ccatgatcat gtgcgggacc cgttccgcta tgaccgggac
     1621 agccgcatca tcaaagaggt gcagccggag gcccgctccg caggaataca ggaaggcgca
     1681 gttctggagt cgctcaatgg cgttccatac accgcagcgt cctgggccga aatcctgaac
     1741 tccgcccgcc ccggcgacat gatggacgta ggctttcagc gcaaagatgg cagccaggga
     1801 gaagccacca tcacgctggt caccctggca ggagacccgc atcttcctcc gctctctgta
     1861 ttcaaagccc agacggtggt attttccatc atctcgctgg cctgtctgct catgggctac
     1921 tgggtggtga tggccaagcc gctggaagcc aatgcctggc tcctgctcgt gctgctcagc
     1981 ggccccagcg tcatcgtttt taatggaagc ggcctcgcca ccggcgtttt cgggttcgcg
     2041 cttcagttct ggtatgtgct gttgcaaatc gccatgcccc cggccctcct gctctttggc
     2101 atctatttcc ctgagccttc gcggattgac gccaggtttc cctggatcaa atggatcgtg
     2161 ctgggaccac tgctggtttg tgccctcatc ggcttctcgg ccttctatgg cgagttttac
     2221 ggcagcggca atcctgcatg gcttgcgcag gccgcaacct gggccggaca cgatcaggat
     2281 ttcctgaacc tgctgtgtgt gctgctgtac ctggccttca cagtagacaa gctccgcacc
     2341 gcctcgactg aggatgcaag gcgcagactg cgcgtgctga cagcaggaat gagcgtaagc
     2401 cttgtggcgg tgctcgtcat cttcatcatc ctgccgcact tcggcatcac agccacaaat
     2461 tcgcggctat tatggctcac ctacgtaggc attctctgcc tgctgcttgc gccctttact
     2521 ctcgtctatg tcgtcctcgt ccagcgcgcg atggatgtgc gcatcctcat tcgcatgggc
     2581 gcgcgttacg cgctggccaa ggcgacgctg tggttcgtgc agattgccct gattaccgtc
     2641 ctggcggtgg agctgctgct gcccgtcttt gggaaaaagc agcaaagccc gatgggcgtc
     2701 atcggcgtgc tggtctttct tgcgattgtg ctggtcctgc gctccggcct gcaaaagcgc
     2761 gcgcagcaat ggattgaccg caggtttttc cgcgaagcct atgatgccga gcgtgtgctg
     2821 aatgatctgg ccgaggaagt ccggcgcttt actgagaccg ggccgctgct tgaaaccgtc
     2881 gccagacgca tcgcagagac cctccacgtc agccagatcg ccatgcttct gcggcgcggc
     2941 gagcagtttg tgctgcaaaa gtccatcggc ctttccacac agaacggact ggtgcttccc
     3001 gtacagtcct cgtctgtccg ctatctcaca aactccaacc agccggcgcg gctttaccgt
     3061 gatgacccgg acgcatggta tctgatggcg ggcacggccg agcgttacac actcgaccag
     3121 atcaacgccg agcttctact gcctctgccg ggacgcaacc gcatgatggg cgtcatggcg
     3181 cttgggccga agcgatctga ggcagcgtac tcctcagcgg acatgaagat gctccaggcg
     3241 ctggcttcgc agacagggct ggcgctggag gtcagcgagc tggcgcggtc gctcgcggca
     3301 gaggcagcgc agcgcgaacg cgcgaaccgc gaaatggaga tcgcccgcga agtgcaggag
     3361 cggctctttc cgcaggagat gccgcagctc ccaggggcca gcgtcgctgg cttctgccgc
     3421 cccgcgcagg gcgttggcgg agattactac gacgtgattg acatgggaga tggccgcatc
     3481 ggcctcgcca ttggcgacgt ctccggcaaa ggcatttcag ccgcgctgct catggccagc
     3541 ctgcgcgcat cgttgcgcgg agtcaccctc gacggcccgc gcgattacgc aagactgatg
     3601 cacaaagtca accgtcttgt gtacgaggct tcggcatcga accgctatgc cacatttttc
     3661 tttggctcct atgatccggc aacgcacatg ctggaatgcg tcaatgcggg gcacaatccg
     3721 cccatagtgt tgcgcggcaa agaagtcatc cgcttaaatc cggacgggcc tgtcgtaggg
     3781 cttctcccct ctgcacccta tacagagcag aaaatccaac tcagggcagg agacgcgctg
     3841 gtcctttaca ccgacggcat cagcgaagcc atgaacaaga ccgatgaaga atggggcgag
     3901 gagcggatga tcgccgccgc acagactgcc cgcgacaaaa ccgcctgcgc gctgctggaa
     3961 accatcttcg ccgccgccga cggcttcacc gccggagcac cgcaacacga tgacatgacc
     4021 ctgctcgttc tgaagatgga cgaataagag tggaattacc acaaactctc gtcatccccg
     4081 ctgaaccgca aggagactca gaagagtccc tggcaatata aggacttagc caatacagct
     4141 gagatttttc tcgtcccccg gaatgacggg ccatcctttt tgtgcatcgc agtaaaacga
     4201 aaaatgccgt aagctcccgg ggcgtatcca accagtgccc gcctgcgcca ctttccagta
     4261 attctgtgca ttttcaccgc gaaaaaccag ctatactgcg cgaagaacac tttttgtatt
     4321 tcccccaggt taaagaggta ccctgatgtc cactgttgca ctgaaagaag ccgaactccc
     4381 gcttgccgcc gatgatttca atgccctcga gcagcgcatt ctgcgtaccg ttgagctgct
     4441 caaaaaagag cgcgaggccc gcgccgccgc cgaacagaac gccgcaacgc tgcaacagct
     4501 tcttgatgag caatctctgg aactgagcaa gacccaggac cacgtcaaag cgcttgaaca
     4561 ggagcgcgaa gctgcgcgtc agcgtgtcga gcgcatgctg aagcaactgg atgaaatttc
     4621 tgcctgagaa atatcgaaca ggagcaatca gtagcgaatg gctaattctc agcaagaaac
     4681 caaggatgca gcgcagtttg tcacagttga tatttacgac caggcgtatc gcctgcgcgg
     4741 accagacccg gcctacatcc aaaagctcgc cgaaatcgta gacaccaaaa tgcgcgcggt
     4801 cgccgcgcag ggcaagacag tagactccct gcgcgtcgcc gtgctcgccg ccctgaatat
     4861 cgccgacgag atgtccgctc tcgaagagcg ctataagcag ctcaccggca gcgcgaaaga
     4921 aacgattcgc aaccgtgcca gcaggctcac cggcctgctc gactcggtct taagcgaaga
     4981 acgcaaaatc agttgaagat tccgggctga cttgcaatcc ttctccgcac ccgctagcat
     5041 ccatgtagaa accggcctgc aaagcaggtg gttgtgattc atgccggttg aacctacatt
     5101 cattgaacag ggagctcgac tcgacaattc ggttcggtgc gcaatgtccg ccagtccgga
     5161 atgcctaaag agccgcgggg agttcccacc gtcttaggac gggttcaccg gcgcatcgct
     5221 ccacggcccc gcgggtcggt tttgtttttc ctgcgatttt gtacccacgc ctcttccagt
     5281 tcggccacat cgccatacct gcttacggcg tatttgcggc gctggccatt ctcgccgcgc
     5341 tggcggtcag cacacagaca gcacgccgcc tctcactcaa tcctgaaacg gtctggaacc
     5401 tcggcctgac cggcattttc accgcgctca tcggcgcgcg tctgcttctg gccctgttcc
     5461 acccgcgaga ttttctcgcc catccgttct ggatgatggg tctggtcagc gtccgcagca
     5521 acactgcctt ttacggaagc ctggtgctcg ctatttgtat ctgttgcgga tacatattcg
     5581 ccagtcgcct gccgctgcgg cgcacgctgg actgcatcgc ccctgccgcc gctctgggac
     5641 tcggcattcg cgccctcgga gcattcgccg ccgggtccga ctacggctcg ccgacgtccg
     5701 ctccctgggg agtcacctac acacaccgcc tcgcttcgct ctggtcaggc acaccgctgg
     5761 ggacccgcct tcatcccgtg cagctctatg aggcggccgt gctcttcgcg ctcttcgccc
     5821 tgctgatgtt tctgttgccg cgctaccaac atgccggtga cctcgccgcc ctgttcctct
     5881 tcgtctacgg agcagcgctg tattttctgg atttctgccg tggcgcccac agctttgtgt
     5941 ttgcggaatt tctttccatc gcgcagattt gcggtatttt atctgttctt gtgggcttag
     6001 ttcttttttg gcaaacgtcg ggaaggcagc aaaatcgcgg tccggcaaca ggaaagattt
     6061 cctgaaactt acgcctgata tcccaacccg gccagcacgc cgcgcatata cgcaaagctc
     6121 ttcgtcactc ccggcatcgg gtcatcgccg tggatctcat actccaggtc cacatggccc
     6181 ttgtaccgga tggcgatcag cgcctcaaaa atagcgcgga cgggcatctt gccatctcct
     6241 acggccacct ggctggcctt atcgcgcaag tcggccaggt ctttcatatg aatgtcaaat
     6301 acgcgcgggc caactttgcg gatggcctcc acaggatcag tacctgtgcg ggcgcagtgt
     6361 cccacgtcaa tgcagcatcc catgcgcggg tccatgttcc gaatgtcctt caaaatgtcc
     6421 agcggcgacg gccatatctt gtcttccggc ccgtggttat ggatagcgaa gcggatgtca
     6481 tactccttca caaatttctc aatccgtggc agcgccctgg gcaatgggtc gccggcaaca
     6541 atcaccttca ctccggcacg tttcgcgtac tcaaatttcg tgcggatgtc atcgtcctcg
     6601 tccttgggaa aatacacagc gcccacagcg gtgagcttga tgccattcgc agcgtagtcg
     6661 cgcagggccg catcggtcgc ctccggcgga gacataggca gatggtcctt cacgtccttt
     6721 gcgttcaggt ccgttagcct gagctctttc at
//
LOCUS       DASRBV010000089         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_308636, whole genome shotgun sequence.
ACCESSION   DASRBV010000089 DASRBV010000000
VERSION     DASRBV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308636"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFA02_03615"
     CDS             complement(<1..620)
                     /locus_tag="VFA02_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZU08964.1"
                     /translation="MSTEAKCPFHEGKIAGTSNRDWWPNQLNLSILHQHSPLSDPMGE
                     DFNYAKEFKSLDYPALKKDLLALMTDSQDWWPADFGHYGPLFIRMAWHSAGTYRIYDG
                     RGGGGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGKKISWADLMILAGNVALESM
                     GFKTFGFAGGREDTWEPDQDVNWGYETTWLGNDKRFSEERELTNPFG"
     gene            784..1695
                     /gene="corA"
                     /locus_tag="VFA02_03620"
     CDS             784..1695
                     /gene="corA"
                     /locus_tag="VFA02_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZU08965.1"
                     /translation="MSEGTPCSLYNASSTMEWHNITGANSPELDQLAQQYHLHPLHIE
                     DCRQRLESPKIEEGESYLFTVIKCVELKPAGGIDAHDICLFLGRDFLISVIEADDTDT
                     QQRIERLRASNNHPRADQLYYRIMDSTADAYFPVLDKFTEMIDALEDEAMEAATPKTL
                     SRIFEIKRILIDLRRIFVHMRDVCAHLQRVDSPYIKRDMWPFLRDIYDHIARNLDQVE
                     VMRDLLSGCLDVYLSSVANRTNQVMKVLTVLSTIALPALVISGIYGMNVKGLPASEAQ
                     HGLWFVLGAMAACTVGLLWILRRFGWL"
     gene            1760..>3552
                     /locus_tag="VFA02_03625"
     CDS             1760..>3552
                     /locus_tag="VFA02_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU08966.1"
                     /translation="MGGRFFFSGISCAFLLCLSSSGAAQTSTYPFQNPQLPVEQRISD
                     LLSRMTLDEKISALSTDPSVPCLGVVGTEHVEGLHGLALGGPGGWEGRFKPVIPTTQF
                     PQARGLGQTWDPELIEKAAAIEAYETRYADGKYHRGGLVVRAPNADLSRDPRWGRSEE
                     SYGEDPFLVGTIAVAFIKGLQGNDPHYWMTASLMKHFLANSNEDGRDGSSSDFDERLF
                     HEYYSVPFRMGVEQGGSNAFMTAYNAWNHVPMAAQPVLHDVVMKDWGFNGIICTDAGA
                     LTNMVTHHHYSKTMPEAAAAAIHAGINQFLDNYKQAVRDALNQKLITEHDIDENLRGV
                     YRVMIRLGMMDRHSMVPYSMIGISNLEKGDPWNWPEHKALARRVTDESIVLLKNEHDT
                     LPLDASKLKSIAVIGPYANIVALDWYSGTPPYTISPLEGITKRAGNSVKVQFATGNDL
                     NGAADLARKSDVAIVVIGNHPTCNAGWAKCPLPSDGKEAIDRKNLTLEQEAIAKAVFA
                     ANPHTIVVLNASFPYTTTWTQEHIPAILEMTHNSEEEGTGLADVLFGDYNPAGRLTQT
                     WVASMDQLPPMMDYDIRHGRTYMYLKTRPLYA"
BASE COUNT          814 a         1025 c          918 g          795 t
ORIGIN      
        1 ccaaaaggat tcgtaagctc gcgttcttct gagaagcgct tatcattccc cagccacgtc
       61 gtttcatagc cccagttgac gtcctggtcc ggctcccacg tatcttcgcg tcctcctgca
      121 aaaccaaagg ttttaaagcc catggattcc agcgctacgt tcccggcgag gatcatcagg
      181 tcggcccacg aaattttttt tccgtacttc tgcttgatgg gccacaacaa acggcgtgct
      241 ttgtccaggc tcacattgtc cggccaacta ttcagaggag caaatcgctg ctggcctctg
      301 cctccaccgc cgcggccgtc atagatgcga tacgtaccag cgctgtgcca cgccatgcga
      361 atgaacaagg gaccgtaatg gccgaaatcg gccggccacc aatcctgcga gtctgtcatg
      421 agcgcaagaa ggtccttctt caaggccggg taatcgaggc ttttgaactc tttggcgtag
      481 ttaaagtcct cgcccatggg atcggacaac ggtgagtgct gatgaaggat gctcaggttc
      541 aactggttcg gccaccagtc acggttcgat gtgcctgcga tctttccttc atgaaacggg
      601 cacttcgctt cagttgacat gcgttctctc ctttaggttc tggccaggtt tcagcacatg
      661 aatgagtgcg tacgtttact gctgcgtctg actgacagat gactgtatca ccccgttaaa
      721 agatagttcc aatacatatt tcccatagga tgtataggca gcatctatag acatcctgat
      781 ggaatgtccg aaggaacgcc atgcagcctt tacaatgctt ccagcaccat ggaatggcac
      841 aacattaccg gcgcgaacag tccggaactc gaccaactgg cccagcaata ccacctgcat
      901 cctctgcata ttgaggactg ccgtcaacgg ctggaaagcc caaaaatcga agaaggcgaa
      961 agctatctct ttaccgtcat taagtgcgtt gagcttaaac ctgctggcgg catagacgcg
     1021 cacgacatct gcctgtttct tggccgcgat tttctcatct ccgtcatcga agctgacgac
     1081 acagacacgc agcagcgcat cgaacgcctg cgcgcttcga acaatcatcc acgggccgac
     1141 cagctttatt accgcattat ggattccacc gccgacgcct attttcccgt cctcgacaaa
     1201 tttactgaga tgattgacgc gcttgaagac gaggcgatgg aggcggccac accgaaaacg
     1261 ctctcccgca tttttgaaat caagcgtatt ctgattgacc ttcgccgcat cttcgtccac
     1321 atgcgcgacg tttgcgcgca tttgcagcga gtggattcac cttacatcaa acgcgacatg
     1381 tggccgtttt tgcgcgatat ttacgaccac atcgcccgca atctcgatca ggtggaggtg
     1441 atgcgtgatc tgttgagcgg ctgtctcgat gtttatcttt cgagcgttgc caaccgcacc
     1501 aatcaggtca tgaaagtgct tacggtcctg agcacaattg ccttgcccgc ccttgtcatc
     1561 tctggtattt acggaatgaa cgtgaaaggg ctgcctgctt ccgaggcgca gcacgggctg
     1621 tggttcgttt tgggagcgat ggcggcatgc accgttggcc tgctctggat tttgcgcagg
     1681 ttcggctggc tttgacattc ccctcatcat ttcttacgat tgtctctacg cattaaaacg
     1741 ttttatcgga gaagacgctg tgggcggacg ttttttcttt tcaggtattt cctgcgcttt
     1801 tttactttgc ctctcttcat ccggtgcagc acaaaccagc acgtatccgt ttcagaatcc
     1861 gcaattaccg gtagaacagc gcatctccga cctgctgtca cgcatgaccc ttgacgaaaa
     1921 aatcagcgct ctcagcacgg accccagcgt gccatgcctg ggagttgtgg gaacagaaca
     1981 cgtcgagggg ttgcatggac ttgcgctggg aggacctggc ggttgggaag gccgcttcaa
     2041 gccggtgatt ccgacaacgc agtttcccca ggcgcgcgga ctgggtcaga cctgggaccc
     2101 ggagctgatt gaaaaagccg ctgccatcga ggcctacgaa acgcgctacg ccgatggcaa
     2161 atatcatcgc ggaggtcttg tcgttcgcgc tccaaatgct gacctcagcc gtgatccacg
     2221 ctgggggcgc agcgaggaat cctatggcga agatccgttt ctagtgggga cgatagcagt
     2281 tgctttcatc aagggacttc agggcaacga tcctcattac tggatgacgg cttcgctgat
     2341 gaagcacttc ctcgccaaca gcaatgaaga cggacgcgac ggatcctctt ccgacttcga
     2401 cgagcgcctg tttcacgaat actattcagt cccctttcgc atgggcgttg agcagggcgg
     2461 ctccaatgca ttcatgaccg cctacaatgc gtggaaccat gttccgatgg cggcgcagcc
     2521 tgtgcttcac gatgtcgtga tgaaagactg gggatttaac ggcatcatct gcaccgacgc
     2581 gggcgcgctg accaacatgg tcacgcacca ccattattcg aaaacgatgc cggaggccgc
     2641 agcagcggcc attcatgctg gcatcaacca gtttctggac aactacaagc aggctgtccg
     2701 cgacgccctc aatcagaagc tcatcaccga acatgacata gatgaaaacc tgcgtggcgt
     2761 ttatcgcgtc atgatcaggc tgggcatgat ggaccggcac tcgatggtcc cctactccat
     2821 gatcggcatc agcaatctcg aaaaaggcga cccgtggaac tggcccgagc acaaagcgct
     2881 ggcgcgccga gtcacagatg agtccatcgt actgttgaag aatgagcacg acacgctacc
     2941 gctcgatgcg tcaaaactga agtccatcgc agtcatcgga ccttacgcaa acattgtggc
     3001 gcttgattgg tacagcggca cgcctcctta caccatcagt ccgctcgaag gcattacaaa
     3061 gcgcgcaggc aacagcgtaa aggtgcaatt cgccaccgga aacgacctga acggagcggc
     3121 agatctggcg cggaaatccg atgttgcgat tgtcgttatc ggcaatcacc ccacctgcaa
     3181 tgcaggatgg gcgaagtgcc ctttgcccag cgatggcaaa gaggccattg accgcaaaaa
     3241 ccttaccctc gaacaggaag cgattgcaaa agctgtgttc gcggcaaatc ctcatacgat
     3301 cgtcgtactg aacgccagct ttccatacac gaccacatgg acgcaggagc acattccagc
     3361 cattctggaa atgacgcata acagcgagga agaaggcacc gggctggccg acgttctctt
     3421 tggtgactac aaccctgcag gacgcctgac acagacctgg gtcgcctcca tggaccagct
     3481 tccgcccatg atggactacg acatccgcca cggacgtact tacatgtatc tgaagaccag
     3541 gccgctctat gc
//
LOCUS       DASRBV010000090         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_561674, whole genome shotgun sequence.
ACCESSION   DASRBV010000090 DASRBV010000000
VERSION     DASRBV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561674"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFA02_03630"
     CDS             complement(<1..701)
                     /locus_tag="VFA02_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HZU08967.1"
                     /translation="MPASELKYNSGFGNEFATEAVAGALPQGQNAPQKHPLGLYTEQF
                     SGTSFTSPRHINRRTWTYRIRPSVTHKPYVEAPMKLIRSGPFTEVPTPPNQLRWDPVS
                     VPKEPADFVDGIITMGGNGDPAMQTGVGIHVYAANKSMEDRFFYNADGEMLVVPQDGA
                     LRFHTELGIVGVAPGEICVLPRGLKFRVVLEEKAARGYICENYGQQFRLPELGPIGAN
                     GLANARDFLYPHAAY"
     gene            805..2304
                     /gene="glpK"
                     /locus_tag="VFA02_03635"
     CDS             805..2304
                     /gene="glpK"
                     /locus_tag="VFA02_03635"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZU08968.1"
                     /translation="MSYILALDQGTTSSRAILFSQNGGIVAQAQQEFRQIYPDGGWVE
                     HDPFDILTTQLSSAIEALTRAGARPRDVAALGITNQRETVIVWDRETGRPIYNAIVWQ
                     DRRTASACRQFVDEGAEKMVVSRTGLLIDPYFSATKIAWLLENVLGARERAEAGKLAF
                     GTVDSWLIWNLTSGKKHVTDYTNASRTMLFNIAEGTWDDEMLKLFNIPRQMMPEVVWS
                     SEKIGEVTTTLGLGSIPIAGIAGDQQAALFGQLCLNPGDAKNTYGTGCFLLQNIGQKF
                     VASKNRLITTLVCSLDRRLEYAFEGSIFIGGAVVQWLRDNLKLIQKSSDVEALANTVS
                     DSDGVVLVPAFVGLGAPHWDAHASGLLIGLRRSTQPGHIARAALESIAFQVADVMDAM
                     NSESGNAFSELRVDGGAAVNDMLMQFQADLLGIPVVRPSVTETTALGAAYLAGLAVGF
                     WDSPKQIWSERKNDASFRPKCGRKEIMAMRERWRNAVERSKRWTEDASA"
     gene            2301..>3638
                     /locus_tag="VFA02_03640"
     CDS             2301..>3638
                     /locus_tag="VFA02_03640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZU08969.1"
                     /translation="MIREQMIAQITRRTTPWDIVVIGGGATGMGIAVDAATRGFDALL
                     LEAKDFGKGTSSRSTKLVHGGVRYLEQGNVSLVMEALKERGILRQNAPHLVNDLAFIV
                     PNYSWWEAPFYGIGLKLYDMLAGKYGFGKSRLLSCEEVLERLPTLQKEGLRGGVIYYD
                     GQFDDSRLLIHLAATAADHGAVLVNYMPVTGLTKDDEGYINGVIARDEEGGQQYTIAS
                     RVVINATGMFTDQVRHMADPEVPSMVEPSQGIHLVFERSFLSGDTAIMVPRTSDGRVM
                     FAIPWHQHTLVGTTDTPIEQPSYEPRPFEEEIRFILETAGQYLSRPPKRSDVLSVYVG
                     IRPLVKATGAAGKTSALSRDHTIHVDNSGLLTITGGKWTTYRHMAEDCVDHAITLGRL
                     REEPCVTRTLKIHGYRKHVDDLGDLWVYGSDADAVLQIAARDPQLGQPLHPALP"
BASE COUNT          815 a          969 c         1075 g          779 t
ORIGIN      
        1 tcataggctg catgcggata gagaaaatcg cgcgcgtttg ctaatccgtt ggcgccgatc
       61 ggcccaagtt caggcaagcg gaactgctgt ccgtagtttt cgcagatgta tccgcgggct
      121 gctttttctt ccagcacaac gcgaaacttc agtccgcgcg gcaaaacgca gatttcacca
      181 ggagcaacgc caacaattcc aagctctgta tggaagcgca gcgcgccgtc ctgcgggaca
      241 acgagcattt cgccatccgc gttgtagaag aagcggtctt ccatagattt gtttgcagca
      301 taaacatgaa tgccgactcc agtttgcatg gccggatcgc cattaccacc catggtgatg
      361 attccgtcca caaagtcggc tggttctttc ggaacagaca ccgggtccca tcgcagctgg
      421 ttgggcggtg tagggacctc agtaaatggc ccgctgcgaa tcagcttcat cggtgcttct
      481 acatacggct tgtgcgtgac cgaagggcga atgcggtagg tccaggtgcg gcggttgatg
      541 tggcgcggag aggtaaagga cgttccgctg aactgctccg tgtagagtcc gagcggatgc
      601 ttttgcgggg cattttgccc ctgtggcagc gcgccggcaa cggcttcggt agcaaattcg
      661 tttccaaatc cagagttgta tttcaattcg gaagcgggca taggtctctc cattgaaaac
      721 tctataagct atctgctggc atggtgattt ggcaaatgct tgcgcaaatg ccgcggacat
      781 tgtttactca tctttgccga aactatgtca tacattctcg ctctggacca gggaacaacc
      841 agctcacgcg ccattctctt ctcgcagaat ggcggcatag tcgcccaggc gcaacaggaa
      901 ttccgtcaga tttatccgga cggcggatgg gtggagcacg atccgtttga cattctgacg
      961 acacaactca gctcagcgat tgaggcgctg acacgggccg gagcgcgtcc gcgggatgtg
     1021 gctgcgctcg gcatcaccaa ccaacgcgag accgtcattg tgtgggaccg cgagacgggc
     1081 agacccatct ataacgccat tgtctggcag gaccgccgca ccgcttctgc ttgcagacaa
     1141 tttgtggatg aaggcgcgga aaagatggtt gtcagcagga cgggcctgct gattgatccc
     1201 tatttttccg caacaaaaat tgcatggctg ctggagaatg ttcttggcgc ccgcgaaaga
     1261 gctgaagcag gcaaactggc tttcggaacg gtagacagtt ggctcatctg gaacctgacc
     1321 agcggcaaaa agcacgtgac tgactatacc aacgcgtcgc gcacgatgct cttcaatatc
     1381 gctgaaggta cctgggacga cgagatgctg aagctgttca atattccgcg ccagatgatg
     1441 ccggaagtgg tctggtcaag cgaaaagatt ggagaagtga caaccacgct cggccttggt
     1501 tcgattccta ttgcagggat tgcgggcgac cagcaggcgg cgctctttgg acagctatgc
     1561 ctgaatccgg gcgatgcgaa aaacacctac ggcacgggat gctttctgct gcaaaacatc
     1621 gggcaaaaat tcgttgcatc gaaaaaccgc ctcatcacta cgctggtttg cagccttgac
     1681 cgcagactcg aatatgcctt tgaaggcagt atttttattg gcggagcggt tgtgcaatgg
     1741 ctgcgtgaca acctgaagct catccagaaa tcgtccgacg tagaagcact ggccaacact
     1801 gtttccgatt cagatggcgt agtgctggtg cctgcgttcg tcggtctcgg cgctccgcat
     1861 tgggacgcgc acgccagcgg acttctgatt gggctgcgtc gcagcacgca accagggcac
     1921 attgcccgcg ctgcgctgga aagcattgcc tttcaagtgg ccgatgtgat ggacgcaatg
     1981 aattccgaat caggcaatgc gttttctgag cttcgcgtag atggcggagc tgccgtgaat
     2041 gacatgctga tgcagtttca ggccgacctt cttggcattc cggttgtgcg cccgtcagtg
     2101 acagaaacaa cggcgcttgg cgcggcctat ctggctggac tggcagttgg tttctgggac
     2161 agcccaaagc aaatctggtc ggagcgaaag aacgatgcga gctttaggcc aaagtgtggc
     2221 cgcaaagaaa ttatggccat gcgagaacgc tggcgcaatg ccgtggaacg cagcaaacgc
     2281 tggacggagg acgcgagcgc gtgattcgtg aacagatgat tgcgcagatc acgcgtcgca
     2341 caactccgtg ggacattgtc gtgattggcg gcggcgctac aggcatgggt atcgctgtgg
     2401 acgccgcaac gcgtggcttt gatgcgctgc ttctcgaagc gaaagacttc ggcaagggca
     2461 catcgagccg cagcaccaag cttgtccacg gcggagtgcg ttatctggaa cagggcaacg
     2521 tctcactggt gatggaggca ctgaaggaac gcggcattct gaggcagaat gcgccgcatc
     2581 tggtgaacga tctcgcattt attgttccaa attactcatg gtgggaagcg cctttttatg
     2641 gaatcgggct gaaactctat gacatgctgg ctggcaagta tggattcggc aagtcgcgcc
     2701 tgctctcctg tgaagaagtg ctggaacgcc tgccgaccct gcagaaagaa gggctgcgtg
     2761 gaggcgtcat ctactacgat ggccagtttg atgattcgcg cctgctgatt catctggcgg
     2821 ccacggctgc agaccacggg gccgtgctcg tcaactacat gcctgtcacc ggactcacaa
     2881 aggatgacga gggttacatc aacggcgtca tcgcgcggga tgaggagggc ggccagcaat
     2941 acactatcgc atcgcgcgta gtcatcaacg ccacaggaat gtttacagac caggtccggc
     3001 acatggccga tcccgaagtc ccctctatgg tcgagccaag tcagggcatt cacctggtct
     3061 tcgagcggtc attcctttcc ggggacacag ctatcatggt cccccgcacg agtgatggac
     3121 gcgtgatgtt cgctattccc tggcaccagc acacgttggt cggcacaacg gacacgccga
     3181 ttgaacagcc ctcttatgaa ccacgcccgt ttgaggagga aatccggttc attctggaaa
     3241 cggcaggaca atacctaagc cgtccgccca aacgcagtga tgtattgagt gtgtatgtag
     3301 ggattcgtcc actggtgaag gcaacaggag cagcgggcaa gacttctgca ctctcgcggg
     3361 accacactat tcacgtagat aattcaggac tgctgacgat taccggcggc aaatggacaa
     3421 cataccgcca catggcagaa gactgcgtgg accatgcgat tacactcggg cgtctgcgcg
     3481 aagagccttg cgttacgaga acgctgaaaa tccacggata ccggaagcat gtggatgatc
     3541 tgggtgatct gtgggtctat ggatctgatg ctgatgctgt gctgcagatt gcagcgcgcg
     3601 atccgcagct tgggcaaccg cttcatccag cgctgccc
//
LOCUS       DASRBV010000091         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_613274, whole genome shotgun sequence.
ACCESSION   DASRBV010000091 DASRBV010000000
VERSION     DASRBV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613274"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..499)
                     /gene="msrB"
                     /locus_tag="VFA02_03645"
     CDS             complement(98..499)
                     /gene="msrB"
                     /locus_tag="VFA02_03645"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZU08970.1"
                     /translation="MAVEKIHRTEEEWRKLLTPEQFHITREKGTEPPFTGRLYYNHED
                     GIYRCVACGAELFTSDKKFESGSGWPSFWLPANTEAVETHEDLGHGMRRVEVTCARCG
                     AHLGHVFPDGPQPTGLRYCINSAALDFEKSK"
     gene            complement(526..918)
                     /locus_tag="VFA02_03650"
     CDS             complement(526..918)
                     /locus_tag="VFA02_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU08971.1"
                     /translation="MSEKQAVSTPRAPAAIGPYSQAVRIGDFLYTSGQVGLDPLSGTV
                     VEGGIEAQTTRAIENLKAVLEAAGTGLKNVVKTTVFLKNMNDFTAMNGIYGTYFAPDG
                     VVAPARSTVEVARLPKDALVEIEAIARL"
     gene            1055..1510
                     /locus_tag="VFA02_03655"
     CDS             1055..1510
                     /locus_tag="VFA02_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU08972.1"
                     /translation="MPAKAKTKHRSGCPVSIALEVFGDKWSLLIVRDLMVKGFRRYKE
                     FLESGEGIATNILADRLKKLEIAGIVQTEVDENDGRRLNYRLTEKGIDLAPVLLELLV
                     WSARHEETGVPCAVIVEMAKDRAGLLREVRRRWKERDPTPLFPWFEEGK"
     gene            1507..2685
                     /locus_tag="VFA02_03660"
     CDS             1507..2685
                     /locus_tag="VFA02_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HZU08973.1"
                     /translation="MSGVKVLVGTRKGAFILTADGKRDKWEVSGPHFAGWEIYHMKGS
                     PVDPDRIYASQTSGWFGQVIQRSSDGGKSWETPGGGVAKSPEGFPMGESNRFVYDTSP
                     DTGVPLTTHQWYDGTQHPWEFKRVWHLEPSLTDPDTVFAGVEDAAIFRSTDGAHSWHE
                     LPGLRGNGTGPRWQPGGGGMCLHTIVLDPDNPQRMYIAISAAGAFRTDDGGKSWKPIN
                     RGLRSQFLPDEDAEVGHCVHHIAMHPQHPDRLFMQKHWDVMRSDNAGDFWTEVSGNLP
                     TDFGFVIDVHAHEPETIYVVPIKSDSEHYPLDGKLQVFRSRAGGNEWEPLTKGLPQSN
                     CYVNVLRDAMAVDRLDKCGVYFGTTGGQIFASADAGDSWFPIVSHLPPVLSVEVQTLT
                     "
     gene            2920..3375
                     /locus_tag="VFA02_03665"
     CDS             2920..3375
                     /locus_tag="VFA02_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU08974.1"
                     /translation="MIRGITPFLWFRDNAEEAVDHYLSIFRDGMILSVSRANGKALVV
                     SFELEGQKFLALNGGAPSQFTESVSFLVECETQEEVDHLWEKLSEGGQTSRCGWLRDK
                     FGLSWQVVPTALSKLMSDPNPGKANAVMQAMMKMDKIIIADLQSAYQAA"
BASE COUNT          777 a          904 c         1048 g          740 t
ORIGIN      
        1 cccacgggag gagcacaggt cagcttcgat ctggtgggtg cgtcagccgt gctcgtggaa
       61 gatggcgatg caaacggaga cgagaattga ttcacatcta ctttgatttc tcaaaatcca
      121 gagcagccga attgatgcag tagcgcagac ctgtaggctg cgggccatcg ggaaagacgt
      181 ggcccaggtg cgcgccacaa cgggcgcagg tgacctcgac gcggcgcatt ccgtggccca
      241 ggtcttcatg ggtctccacg gcctctgtgt tggctggcag ccagaaactc ggccatccgc
      301 tgcccgactc aaatttcttg tcagaggtga acagttccgc accgcaggcc acgcagcggt
      361 agatgccgtc ttcgtggttg taatacagcc ttccggtaaa gggcggctcg gtgccttttt
      421 cgcgcgtgat gtggaactgc tccggagtca gcagcttgcg ccactcctcc tcagtgcggt
      481 ggattttttc aactgccata tgccccctcc tttctgtttc gatggtcaga gtctggcgat
      541 ggcttcaatc tccaccagag catctttcgg gagccgcgcc acttccacgg tagagcgtgc
      601 cggggccaca acgccatcgg gagcaaaata tgtaccgtag atgccattca tggcggtgaa
      661 atcgttcata ttctttagaa aaaccgtcgt ttttaccacg tttttgagtc ctgttcccgc
      721 cgcttccagc accgccttca ggttttcaat ggcgcgcgtg gtctgcgctt cgattccgcc
      781 ttccacgacg gtcccgctca atggatcaag acccacttgc ccggaagtgt aaagaaaatc
      841 gcctatgcgc acggcttgcg agtatggccc gatggccgcc ggagcacggg gagtagaaac
      901 agcctgtttt tcgctcatgc agactattgt gtcattttcc cgtgaaggac ttccagaaac
      961 gacaagactc tgcgctctgc atgacgcagc cggtctcatg gaatttctat agaatacttg
     1021 caaaacaaaa gtaaattttg atagggtggg ttgcgtgcct gcaaaggcaa aaacaaagca
     1081 caggtctgga tgtccggtaa gcattgcact cgaggtcttc ggcgacaagt ggtcgctgct
     1141 tatcgtgcgc gatttgatgg tgaagggttt ccggcgctat aaggagttcc ttgagtcggg
     1201 cgaggggatt gcgaccaata ttctggccga tcggctgaag aagctggaga ttgcgggtat
     1261 tgtccagaca gaagtggacg aaaacgacgg gcgcagactg aattaccgtc tcacagaaaa
     1321 agggattgat cttgcgcctg tgctgctgga actactggtc tggagtgcgc ggcatgagga
     1381 gacgggcgtc ccgtgcgctg taattgtgga gatggcaaag gaccgcgcag gtcttttgcg
     1441 agaagtgcgg cggcgttgga aagagcgcga cccaacgccg ctgtttccgt ggttcgagga
     1501 gggaaaatga gcggagtcaa agtgctggtt gggacaagaa agggcgcatt catccttacc
     1561 gccgacggca agcgcgacaa gtgggaagtg agcgggccgc attttgccgg atgggaaatc
     1621 taccatatga aaggctcgcc ggtagaccct gaccgcatct acgcatcgca gaccagtggc
     1681 tggttcggcc aggtcatcca gcgttcgagc gatggcggca agagctggga gacgccggga
     1741 ggcggtgtcg ctaaatcgcc ggaaggcttc cctatgggcg agagcaaccg ctttgtctat
     1801 gacacttctc cggacacggg cgtgccgctg acgacgcacc agtggtatga cggcacgcag
     1861 catccgtggg aattcaagcg agtgtggcac ctggagccgt cattaactga tcccgacacg
     1921 gtcttcgccg gcgtggaaga tgcagccatc ttccgctcga cggatggcgc gcactcctgg
     1981 catgagcttc ccggcctccg tggcaacggg accggccccc gctggcagcc cggaggaggc
     2041 ggcatgtgtc tgcacacaat tgttcttgac ccggataatc cgcagcgcat gtacattgcg
     2101 atttccgcag caggagcatt ccgcaccgac gatggcggaa aatcctggaa gcccatcaat
     2161 cgcggcctgc gctcgcagtt tcttccagat gaggacgcgg aggtgggcca ctgcgtgcac
     2221 cacatcgcca tgcacccgca acacccagac aggctcttca tgcagaaaca ctgggacgtg
     2281 atgcgcagcg acaatgcagg cgatttttgg acggaggtga gcggaaacct gccgacggat
     2341 tttggctttg tgattgatgt tcatgcgcat gagccggaga cgatctatgt cgtccctatc
     2401 aagagcgact ccgagcacta tccgctggat ggaaaacttc aggtctttcg cagccgcgcg
     2461 ggcggaaacg agtgggagcc actgacgaaa gggcttccgc aaagcaactg ttatgtaaac
     2521 gtgctgcgcg atgcgatggc agtggaccgt ctggacaagt gcggcgtcta ctttggcaca
     2581 accggcggac agatcttcgc ctcggcagac gcgggcgact catggtttcc catcgtgagt
     2641 catttgcccc cggtgctttc agtagaagta caaacactaa cgtaggagcg atgtctcagc
     2701 gcaaataaat attgaatcta ccgaagacat gaaggacaag ggaacatggg atgtgcaggc
     2761 aagctttggg aagaggcgaa acaactttat agatcgctac gattggaacc gggcgtttcc
     2821 gagtgtgatg cacatattcg caagctacag caactgtgat tttgaactgc gatagagacc
     2881 catacaaggc cagataactg tgcgcccagg agctttctaa tgattcgagg aattactcca
     2941 tttctgtggt ttcgtgacaa cgcggaagaa gccgtagacc attatctctc tatcttcaga
     3001 gatggcatga ttctgagcgt ctcgcgagca aacggtaaag cgctggtagt gagttttgaa
     3061 ctggaaggcc agaaatttct ggcgctcaac ggtggcgcgc cttcgcagtt tactgagtca
     3121 gtctcctttc tggtggagtg cgaaacacag gaggaagtag atcatctctg ggagaagctt
     3181 tcagaggggg gccagacgag ccgctgcgga tggctcagag acaagttcgg cttgtcctgg
     3241 caggtcgtcc caactgcgct gagcaagctg atgagcgacc ccaatcccgg aaaagcgaat
     3301 gcagtcatgc aggccatgat gaaaatggac aagatcatca ttgccgacct gcagagtgcg
     3361 tatcaggcgg cataggttga gcatggtccg tgtcattctt ccacagcatc tgcgcaccct
     3421 tgcgcatgtg aacggcgagg ttttgcttga ggtcgaaggt gcggtaacg
//
LOCUS       DASRBV010000092         8327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_716614, whole genome shotgun sequence.
ACCESSION   DASRBV010000092 DASRBV010000000
VERSION     DASRBV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716614"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VFA02_03670"
     CDS             complement(<1..1646)
                     /locus_tag="VFA02_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HZU08975.1"
                     /translation="MTRFSKKWTALLGALVLSTGLWAQTAQPNKTPWWKGAVFYEIYP
                     RSFQDSNNDGVGDLNGITKRLDYLKSLGVDAIWLTPIYPSPQVDFGYDISNYEDIDPQ
                     YGTLKDFDHLVAEAKKRNIRIIMDLVLNHTSDKHPWFIESSSSKTNPKRNWYVWRDGK
                     GPGQPPNNWESYFGHSAWQYDPKTGQYYYHKFYIQQPDLNWNNPEVRKAMYDVERFWI
                     NRGVAGFRLDAITTLFEDPEMRDEGVVKDTNGKPMINAYGDPVLDDTRTNDLPQVHDV
                     LRELREVADSYKGRDIVLIGETYLRSIDDLRKMYGAHDDELQLPMDMQIGFINKFDVA
                     LFRQRINEAQNDINGNEPLIVFDNHDNPRWDRYGDGKYHDDIGRVIATVLFATRGSAM
                     FYYGDEIGMVTTPPTRKEDVKDPIGITGWPKEKGRDGERTPMQWDDSPNAGFTGPNTK
                     PWLPIPPSYKTVNVAAEEKDPDSMLSWYKQMIELRRTVPALRYGNNIMLNTNDNKVLS
                     WLRILPGQAPVVVACNFTSEPQKISFDLSAQGVTGKHTRTL"
     gene            complement(1796..3427)
                     /locus_tag="VFA02_03675"
     CDS             complement(1796..3427)
                     /locus_tag="VFA02_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08976.1"
                     /translation="MRRILLWCAGIFFTLVISVGVVITILIHRSEPILRQRVIDTLSA
                     RFDSRVELDHFHVYFLNGFQVRGSGLKLWPNQISSDQPLFAVDEFGFRTDWHGLLHSP
                     MTIGLVRLKGLAIHLPPKEERHNLPKLSGSSGGGVKIFVDKILIDSAKLVLGTNKPGK
                     IPLDFDIHNLQLRSVGAGQPMQFHAILVNPKPIGNIDSSGHFGPFDAHSPGDTPVDGI
                     YSFTHADLNSIKGIGGILSSHGKYAGTLDNIVVDGETDTPDFDVDTGGHPMPLHTNFH
                     AIVDGTSGDTYLQPVDAMLAHTHILAKGDVVRAPQGGHNITLDVTVGPSRIEDVLQLA
                     VKTLPPLMSGDLKMKTKFFLPAGPEPVPERLQLKGNFSVSNVLFASDKVQAKVDELSL
                     RSQGHAKEAKEMNKQNPESIQSQMSGQFQLADGKAAFPALKFNVPGADISLQGLYTMN
                     GKEYDFHGNARLTAKVSQLTTGWKSILLKPVDPFFSKNGAGTEVPIRITGSRNSPKFG
                     LDFGHKDENHTQPNLKDKSKDYGIVEKVPKKQQPK"
     gene            3608..5242
                     /locus_tag="VFA02_03680"
     CDS             3608..5242
                     /locus_tag="VFA02_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HZU08977.1"
                     /translation="MRAQVAAPEAPPSVPVTTNINQYIHNAWDTLSRSMVDCHSLIDP
                     KVRTASVLYLPQEVPMPAQVAALKEHCNVRVERLPKRITHIGEIDPRSLKTPGLLYLP
                     NKYVVPGGRFNEMYGWDSYFILLGLLQDKRVELARGMVENFFYEIEHYGSILNANRTY
                     YLTRSQPPFLSSMIRDVYDADKPGKARDEWLEKAYSYAVRDHNLWMSSFHRAGDTGLA
                     RYFDLGEGPVPEQEDDSTYYPDVIRWLLAHPDVHTDYLVEAPDHPDAAEKAKLAQISC
                     DPRISVVCSRAHMDGHWLSHGYYKGDRAMRESGYDPSFRFGPFSGSTQDYAPICLNSL
                     LYKYERDLAWMAEKLGKSNQAKRWNGQGEARKAAINKYLWNAKEGMYFDYNYETHQQS
                     TYRYLTTFYPLWAGLADSEQQKGLEKALHWFEKPGGLAMSTYDSGTQWDLPFGWAPPT
                     WIAIAGLEKAGDIADARRISEEFSHTILTNFDRDHTVREKYDVVHGSSEMSITTGYKS
                     NEVGFGWTNAAYLKMQQLLATTGEKSEQTASDRGNR"
     gene            5208..6140
                     /locus_tag="VFA02_03685"
     CDS             5208..6140
                     /locus_tag="VFA02_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZU08978.1"
                     /translation="MSKQHRIVGIGELLWDLLPGGKQLGGAPANFAVMSARLGNHGVI
                     ASRIGKDQLGKEALARLSAFPADLSYLQTDSQHPTGSVSVALHQGQAEYVFHSPVAWD
                     FLECTPEWRELAQQADAVCFGTLAQRNDVSRKTVAAFLEATAPDCVRVFDVNLRRPHF
                     DAEVIAESLRKATLMKLNDAEMPPVLALLGLALECGLDEASLLKGAKLLLDKFPLSLV
                     CITMGPLGSLLVTCDAHDRHHGFAAKVADTVGAGDAFTAALVHYYLQGAPLSVLNEAG
                     NRWGAWVAAQPGAMPDLPEEVREEIEAGIRQAAG"
     gene            complement(6163..6672)
                     /locus_tag="VFA02_03690"
     CDS             complement(6163..6672)
                     /locus_tag="VFA02_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08979.1"
                     /translation="MKPIYRRLATVIFLLTVSLFASAQSSAPVILHAGDAAKILPDAV
                     FFRGQSAPVQARNSAGVRFPDGMYTLATLVDNSGYSTGIQQKYQAYFITEVPLDFSGH
                     TLHPGAYGTGFVNGKFLVMDVAANDIFSAPAVHDNEIKRPTPLQLVADGDHYRLYFGR
                     NYVVFSRAK"
     gene            complement(6715..7461)
                     /gene="pyrF"
                     /locus_tag="VFA02_03695"
     CDS             complement(6715..7461)
                     /gene="pyrF"
                     /locus_tag="VFA02_03695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZU08980.1"
                     /translation="MAVFGNIEDIPARDFRSAQGKLIVALDFPSGDKALAFVDQLEGL
                     CRWFKVGLELYLAAGNRIVGQLKEQGFSVFLDLKLHDIPNTVAGAVRSVSSLGVDLLT
                     VHASGGPAMLQAAAEAAASQPQAPRLLAVTVLTSMDAGQLSAIGVSDSPAEQALRLAR
                     AAHRAGIAGLVCSPEEAARMRRELPDSVLVTPGIRPAGAEIGDQKRIATPAAALSAGA
                     DYLVVGRPITQAARPAAAAEAILAEMSQGL"
     gene            complement(7475..8014)
                     /locus_tag="VFA02_03700"
     CDS             complement(7475..8014)
                     /locus_tag="VFA02_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZU08981.1"
                     /translation="MTAIATDTSRRNPKLSTYAGVLLAAVVGLFSVKTGTPVSTSNPQ
                     TPVAPDKSPDTKKDATKKRHWYQIGRASWYGEDFQGQQTASGEDFDMNKLTCAHRSLP
                     LGSLIRVTNLRNHKSVVVRVNDRGPMPQNRVVDLSYAAARFLGFSSKGTAPVRLDLVN
                     PKNDVAQLMYPLTPVTAKP"
BASE COUNT         1795 a         2252 c         2313 g         1967 t
ORIGIN      
        1 agggtccttg tatgttttcc agttacaccc tgcgcagaca aatcaaagct gatcttctgt
       61 ggctcagaag tgaagttgca cgccactacc actggcgctt gtcccggaag aatacgtaac
      121 catgacagta ccttattatc attcgtattc agcatgatgt tgttgccata gcgcagcgcg
      181 ggaactgtgc gccgcagctc gatcatctgc ttgtaccagc tcaacatgga gtccgggtcc
      241 ttttcctccg cggccacgtt cactgtctta taactcggcg gaatcggcag ccacggcttt
      301 gtgtttggtc cggtaaatcc tgcgtttggg ctgtcgtccc actgcatggg agtgcgctcg
      361 ccatcgcgcc ctttttcctt tggccatccg gtaatgccaa tggggtcctt cacatcttcc
      421 ttgcgcgtgg gcggcgtggt caccatgccg atttcatcgc cgtaatagaa catcgcgctg
      481 ccgcgtgtag caaagagtac tgtcgcaatt acacgtccga tgtcatcgtg atacttgccg
      541 tctccgtaac ggtcccagcg cgggttgtca tggttgtcaa aaacaatcag cggctcattg
      601 ccgttgatgt cgttctgtgc ttcgttgatg cgttgccgga agagcgcgac atcgaacttg
      661 ttgataaagc ctatctgcat gtccatcggg agttgcagct catcatcatg cgcgccgtac
      721 atctttcgta aatcgtctat gctgcgcaga taggtctcgc cgatgagcac aatatcgcgg
      781 cctttgtagc tgtcggcaac ctcgcgcagt tcacgcagaa catcatgcac ctgcggcagg
      841 tcgttggtcc tggtatcatc cagcaccggg tcgccatagg cattgatcat gggtttgccg
      901 tttgtgtctt tcaccacgcc ttcatcgcgc atttcagggt cttcaaagag cgttgtgatg
      961 gcgtccagac ggaacccggc aacgccgcga ttgatccaga agcgctccac gtcgtacatc
     1021 gccttgcgca cctccggatt gttccagttc aggtctggct gctgaatgta aaacttgtgg
     1081 tagtaatact gcccggtttt cggatcatac tgccacgccg aatgtccaaa gtaggattcc
     1141 cagttgttgg gtggctgccc tggtcctttg ccatcgcgcc agacatacca gttgcgtttg
     1201 ggattcgtct tcgagctgct cgattcaata aaccagggat gcttgtctga agtgtggttc
     1261 agcaccaggt ccataatgat gcgaatgttg cgcttctttg cttcggccac aagatggtcg
     1321 aagtccttca gtgtgccgta ttgcggatca atgtcttcgt aatttgagat gtcatagccg
     1381 aagtccacct gcggtgatgg ataaatggga gtcagccaga tggcgtccac gccaagtgac
     1441 ttgagataat ccagccgctt tgtgattccg ttcaggtcac ccacgccgtc gttgttggaa
     1501 tcctgaaaac tgcgtggata aatctcataa aacaccgcgc ccttccacca cggcgttttg
     1561 tttggctgcg ctgtctgcgc ccacagtcct gtgcttaaca ccagagcgcc cagcagggcc
     1621 gtccattttt ttgaaaagcg tgtcatgatg tctcccgaaa aattaagaac gatggttttg
     1681 ttttcttatc acctgcatac gcaagaaaca tcattctaat gcctgaaaca agcgaatggt
     1741 tctgccggga gcgtggtttt caaaagaaaa cgttatcgcc tgctgagcag cggtattact
     1801 tcggctgttg ctttttcggg accttttcga caatgccgta atctttggat ttgtctttga
     1861 ggttgggctg tgtatggttt tcgtctttgt gcccgaaatc gaggccgaat ttaggagagt
     1921 ttcggctgcc cgtgatgcga atgggcactt ccgttccagc gccgttcttt gaaaagaacg
     1981 ggtctactgg cttcagcaaa atagacttcc agccggtggt aagttgggag actttcgcag
     2041 tgagccgggc atttccatga aagtcgtact ctttgccatt catagtgtag agaccctgca
     2101 gactgatgtc tgcgccggga acgttgaact tcagagcagg aaaggccgcc ttgccatccg
     2161 ccaactggaa ctgcccgctc atctgcgact ggatggattc ggggttctgc ttattcattt
     2221 cctttgcttc ctttgcgtgt ccctgactgc gtagactcag ctcgtctacc ttggcctgta
     2281 ctttatcgct ggcaaaaaga acattcgaaa cagaaaaatt tcctttgagc tgcaatctct
     2341 ccggaacagg ttctggtccg gcaggaagga agaatttcgt cttcatcttc agatcgccgc
     2401 tcatcaacgg cggcagtgtc tttactgcaa gttgcagcac gtcttctata cgtgacgggc
     2461 caacggtcac atccagcgtg atattgtgcc ctccttgtgg agcgcgcacg acatctcctt
     2521 ttgccagaat gtgcgtgtgc gccagcatcg cgtctacagg ctgcagatag gtgtcgccac
     2581 tggtaccgtc cacgattgcg tgaaaatttg tgtgcagcgg catcggatgg ccgcctgtgt
     2641 ccacatcaaa atcaggagtg tcggtttcgc cgtcaaccac aatgttgtcc agcgtgcctg
     2701 catatttccc atgcgatgag agaatgccgc caattccctt gatcgaattc aggtccgcgt
     2761 gagtaaagct gtagatgccg tctaccggag tatcgcccgg gctgtgcgca tcaaacggcc
     2821 cgaagtgtcc gcttgaatca atattgccaa tcggttttgg attgacgaga attgcgtgaa
     2881 actgcatggg ctggccagca ccgacagatc gtaactgcaa attgtggatg tcaaaatcaa
     2941 gcggaatctt tccaggtttg tttgtgccca gcaccagttt ggcgctgtct atcaggatct
     3001 tgtccacaaa gatttttacc cctcctccgc tgctgcctga aagttttggc agattatgtc
     3061 tttcttcctt tggtggcagg tggatggcga gtcctttcag gcggacaagt cctatggtca
     3121 tgggagagtg cagcagtccg tgccagtctg tgcggaagcc gaattcatct acagcaaaga
     3181 gcggctgatc ggatgaaatc tggttcggcc acaacttcag accggagccg cgcacctgaa
     3241 atccgttcag aaaatagaca tggaaatggt ccagttcgac gcggctgtca aaacgggcgc
     3301 tgagtgtgtc aatgacgcgc tggcgcagga tcggctcgga gcggtgaatc aggattgtaa
     3361 ttacaacgcc aacgctgatg accagggtaa aaaaaattcc cgcgcaccat agcaggatgc
     3421 gccgcaaacg gctatgcttt tttgttgtcc cggcagaatg ttttgaggaa ctctgatctt
     3481 gcacgcgaag gcctttcaat aattacggag atatatgcaa ttagaggcac atttcttttc
     3541 tcgtgttgga tgcgcgatca agcggcttgg ctgctgtttt gtcctcgcgg ccctgtgtgc
     3601 ccccggaatg cgcgctcaag tcgctgcacc ggaagcgccg ccatctgttc ctgttacaac
     3661 aaatatcaat caatacattc acaatgcctg ggacacgctc tcgcgttcca tggtggattg
     3721 ccactcgctg attgacccga aagtccgtac cgcttccgtg ctgtatcttc cgcaggaagt
     3781 cccgatgcct gcgcaggtgg ccgcgctgaa agaacactgc aatgtgcgcg tcgagcgtct
     3841 gccaaaacga attacccata tcggagaaat tgatccgcgc tcgctaaaga ctcccggtct
     3901 gctctatttg ccaaacaaat atgttgttcc cggcggccgt ttcaatgaga tgtatgggtg
     3961 ggacagctac tttatcctgc tcgggctgct ccaggacaaa cgggtagaac tggcgcgcgg
     4021 tatggttgag aactttttct atgaaattga acactacgga agcattctta atgccaaccg
     4081 gacctactat ctcacacgct cgcagcctcc atttctttcg tccatgattc gggacgtcta
     4141 cgatgcagac aagcccggaa aggcgagaga tgaatggctg gagaaggctt attcctatgc
     4201 cgtgcgcgac cataatctct ggatgagcag ttttcaccgc gccggggata ctggcctggc
     4261 gcgctacttt gatctcggcg aagggcctgt tcccgagcag gaagacgact ccacttatta
     4321 tccggatgtg attcgatggc tgctggctca tcccgatgtg cacacagatt atcttgtaga
     4381 agcgcctgac catccggatg ctgccgaaaa ggcaaagctc gcgcaaatca gttgtgaccc
     4441 acgtatctcc gtcgtgtgct cgcgggcgca tatggatggc cactggctct cacacggcta
     4501 ctacaaaggc gaccgcgcca tgcgggaatc tggctatgac cccagcttcc gctttggccc
     4561 atttagcggc tccacgcagg actatgcacc tatttgttta aacagccttc tttataaata
     4621 cgaacgcgac ctcgcctgga tggcggaaaa gctgggcaaa tcgaatcaag caaagcgatg
     4681 gaacggtcag ggcgaagccc gcaaagctgc catcaataaa tatctttgga acgccaaaga
     4741 gggcatgtac tttgactaca actacgagac gcaccagcag tccacatacc gctatctgac
     4801 gacgttttat ccgctgtggg ccggactggc tgattccgaa caacagaagg gcctcgaaaa
     4861 agctctgcac tggtttgaga agccgggcgg actggccatg agcacatatg attccggcac
     4921 gcaatgggac ctgcccttcg gctgggcgcc tccaacatgg atcgccattg caggcctgga
     4981 aaaagcaggt gatatcgcgg acgcgcggcg catctctgag gaattcagtc acacaattct
     5041 cacgaatttt gaccgtgacc atacggtccg cgaaaaatat gatgtagtac atggctcgtc
     5101 agagatgagc atcaccaccg gatacaagag caacgaagtc ggatttggat ggaccaacgc
     5161 cgcctacctg aagatgcagc agttgctcgc aaccacagga gaaaagagtg agcaaacagc
     5221 atcggatcgt gggaatcggt gagctgctat gggaccttct gcccggtgga aaacagcttg
     5281 gtggagcgcc tgcaaatttt gccgtgatgt ccgcgcggct cggaaatcat ggtgtgattg
     5341 ccagccgcat cggtaaggac caacttggga aggaagcgct cgcgcggctg tctgcgttcc
     5401 cggcagacct aagctatctg caaacggatt cgcagcaccc aaccggcagt gtttctgtcg
     5461 cgttgcacca gggccaggcc gaatatgtgt tccacagccc cgtagcgtgg gactttcttg
     5521 aatgcacccc ggaatggcgg gaacttgcgc agcaggcgga tgccgtatgc tttggcactc
     5581 tggcccagcg aaacgatgta tcgcgcaaga cggttgccgc gtttctggaa gccactgcgc
     5641 cggactgcgt ccgtgttttt gacgtaaatc tgcgcaggcc gcattttgat gcggaagtga
     5701 ttgctgaatc gctgcgtaag gccacgttga tgaagctgaa cgatgcggaa atgcccccgg
     5761 tgcttgcgct cctgggactc gcgctcgagt gtggcctgga tgaagcctct ctgctcaagg
     5821 gcgcgaagct tctgctggac aagtttcctt tgtctctggt ttgcatcacg atggggcctc
     5881 tgggcagcct tctggtcaca tgtgatgcac acgatcgcca tcacggcttt gctgcaaagg
     5941 tggcagacac agtcggcgcg ggcgatgcct ttaccgctgc gctggtccac tactaccttc
     6001 agggcgcacc tttgtctgtt ctgaacgaag cagggaaccg ctggggcgcc tgggtggctg
     6061 cacaacccgg cgccatgccc gatttaccgg aagaagtacg cgaggaaatc gaagccggca
     6121 tcaggcaggc agccgggtaa aaagagtccc gcctcggacc tgctattttg cgcgggaaaa
     6181 gacgacgtag ttgcggccaa agtacagacg ataatggtct ccgtccgcga ccagttgcag
     6241 tggagttgga cgtttgatct cgttatcatg cacggcgggg gccgagaaga tgtcgtttgc
     6301 cgccacatcc atcacaagga acttaccatt tacaaaccct gtgccatagg ccccagggtg
     6361 cagagtgtgt ccgctgaagt cgagtggcac ctcggtgata aagtatgcct gatatttctg
     6421 ctggattccg gtggagtagc ccgaattgtc caccagggtt gcgagcgtgt acatcccatc
     6481 aggaaaacgg acgcccgccg agttgcgtgc ctgtaccggg gccgactgcc cgcggaaaaa
     6541 tacggcatca ggaaggatct tggcggcatc gcctgcatga agaatcaccg gagcggagga
     6601 ctgggctgat gcaaaaagag acacagtcag gagaaaaata acagttgcca gacggcggta
     6661 gattggtttc atttcctttt ccttgcaaag aagaagtata tgacagggaa caggtcacag
     6721 tccttgtgac atttccgcca gaatggcttc agctgcggct gccggcctgg ctgcctgcgt
     6781 aatgggtctg cccaccacca gataatcggc cccggcggaa agggcggcag cgggagtggc
     6841 aatgcgcttc tggtctccaa tctcggcccc ggcagggcga atgcccggag tcacaagtac
     6901 tgaatcaggg agttcacggc gcatccgggc ggcctcctcg gggctgcata caagtccggc
     6961 gattcctgcc cggtgggcgg cccgcgccaa tcgaagcgcc tgttccgctg gagaatcaga
     7021 cacgccaatg gcggaaagtt gccctgcatc catgctggta agcaccgtga ctgccagcag
     7081 acggggtgcc tggggttgcg atgccgccgc ctcggctgcg gcctggagca tggctggccc
     7141 tccgctggcg tggacagtca gcaggtctac ccccaggctg gagacggaac gcaccgcccc
     7201 ggcaacggta ttcggaatat cgtgcagctt caggtccaga aagacggaaa atccctgctc
     7261 cttgagttgc ccgacaatcc tgtttccggc agccagataa agctccagcc ctactttgaa
     7321 ccagcggcag aggccttcga gctggtcaac aaatgccagg gccttgtcac cggaggggaa
     7381 gtcgagtgcg acaatgagct ttccctgcgc ggaacggaaa tcacgcgcag ggatgtcctc
     7441 tatatttcca aaaacggcca tcacgaagat gattctacgg ctttgccgta actggcgtca
     7501 gcggatacat gagctgcgcc acgtcgttct tcggatttac caggtccagc cggaccgggg
     7561 ccgtgccttt gctgctgaaa ccaaggaagc gcgctgcggc gtaagaaagg tcaacgacac
     7621 ggttctgcgg catcgggccg cggtcgttta cgcgcaccac cacagatttg tggttgcgga
     7681 gattcgtgac gcggatgagc gaaccaagcg gcaggctgcg gtgcgcgcac gtcagtttgt
     7741 tcatgtcaaa gtcttcgccg ctggccgtct gttgtccctg gaagtcctcg ccataccagg
     7801 aggcgcgtcc aatctggtac cagtggcgct ttttcgtggc gtccttcttc gtatccggac
     7861 tcttgtcggg agctacaggg gtctgcgggt tagaggttga gaccggcgtt ccggtcttta
     7921 cgctgaaaag gccgacaacg gctgccagca acactccagc ataggtcgaa agcttcggat
     7981 tccgtctgct cgtatcagtt gcgatagcgg tcatagtcct cctttaggaa ttgtaagaag
     8041 gcagatgacc taagtcaaac aaaattaatg atttgctcca ttttctgctt gcaaaaatac
     8101 atcacttgtg gtagctgttc tgcctgcgaa aaactgccac atgttcctct atgtcagcat
     8161 ctggacatgt gaccttatga tgatagggtc atgaatcagg ctgctcctgc atctctcgaa
     8221 agagacgttc ggcccatgcc gcttgccctg accattgccg gcttcgaccc ctcgtcaggc
     8281 gccggtatct ccgcggattt aaaggtattt gcggcccatc gggtcta
//
LOCUS       DASRBV010000093         6193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_613380, whole genome shotgun sequence.
ACCESSION   DASRBV010000093 DASRBV010000000
VERSION     DASRBV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613380"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFA02_03705"
     CDS             complement(<1..247)
                     /locus_tag="VFA02_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZU08982.1"
                     /translation="MAKSRFRLLLHWIPALLAVLVIVIESTGFFSSGNTSRWLLPVWE
                     KLFGPVTPEQWATIHFYIRKAGHFVGYGLVSLAFFEGW"
     gene            complement(255..581)
                     /locus_tag="VFA02_03710"
     CDS             complement(255..581)
                     /locus_tag="VFA02_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU08983.1"
                     /translation="MLDYEISPFQLAELLKGPQKPVLLDVREPQEYALARIDGSILMP
                     MGEVPGRAHHELDPDAHIVTICHHGVRSMNVAVWLRNQGFDHVQSLRGGIDAWSSVMD
                     PAVPRY"
     gene            683..892
                     /locus_tag="VFA02_03715"
     CDS             683..892
                     /locus_tag="VFA02_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08984.1"
                     /translation="MVMLHNGQCGLCVHFGEPHPATPMLIQIHNSKQAPETFLDDCGH
                     PKHAPLHLKVSAMSGCDGFEPAKAA"
     gene            955..2640
                     /gene="recN"
                     /locus_tag="VFA02_03720"
     CDS             955..2640
                     /gene="recN"
                     /locus_tag="VFA02_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZU08985.1"
                     /translation="MLLELRAENYAVIDHAIAVFGPGLNLLTGETGAGKSILVDALAL
                     LMGEKASSDVVRHGADKAVVSCVFEGTPGAESVLEANGIDSEGHEMILRREIYANGKG
                     RVFINNQPATVTALRQLAPELALVHAQSETLGAFDQAQQRGLLDRFGAISTENVGLLY
                     EKWREIQQKLADLERDEQDRLRMVDLWSFQHREIGEIKPTEGEDEALETERRVLSNAE
                     KLYTAAMSAHELLYEGGTSAETALRAALKQLEELARYDPQFQDAIQQISSARATVQDV
                     SATVRDYAEDIQASPERLAEIEDRLAALDRLKRKYGPTLAGVIAYGEEVAARLNEVEN
                     RDEIVKSLRVELKAAEDEYRNAAQLLTNLRGEAAKKLEKLAEAHINDLAMKVHFAVMV
                     TPDEDQAHWTAYGWDTVEYRIATNAGEPLKPLDEIASGGEMSRVLLALKVSVEEGANK
                     AQKKKTQVPRTLVFDEIDIGIGGRAAEAVGQKLKALSRAQQVLCVTHLPQIAAFADQH
                     FLIEKKEQGGRTKTSVRLLEDHERVEEVARMLSGAKLTDASLKHAEQMVKASR"
     gene            complement(2734..3114)
                     /locus_tag="VFA02_03725"
     CDS             complement(2734..3114)
                     /locus_tag="VFA02_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU08986.1"
                     /translation="MLAYQDVNQAAAWLCEAFGFSVRLRIGNHRIQLNAGDGAIVVRE
                     IRPSEVAATLGVGFSVMVRVEDVDAHCNHARNHGAQIAQEPVTHSYGERQYNAIDFAG
                     YSWTFSQSVADVHPQDWGGTAEEL"
     gene            3226..5697
                     /locus_tag="VFA02_03730"
     CDS             3226..5697
                     /locus_tag="VFA02_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU08987.1"
                     /translation="MQWPTFESLWLDTKYALRQIFKSPGISLIAVLTLAFGIGANTAI
                     FTLTWAIVLRSLPVPHPEQLVQYGMRNGGTLIGLSGPEYNALRQMQKTSVDLLAWTSD
                     RPSVRTGNDTRREEVQFLTGNAFKTLGLRPYLGNFFSDAQQNAAILSYEYWQQQFHGS
                     SDVIGQSLFVGGHPVVITGVMPEAFTGLTANFHPAVYLPMKFGNVLYGKDYTTAPGHL
                     GFYAMGRLKPGATLTQATAEAKALEPTIRKEADPSGIYLGQFFQSFHLDVQSGRSGIS
                     WVKMAYARPLFVLELLVIFLIVLCCLNAALVMLARVSGRQQEFAVRSALGASRSNLIS
                     QVLLETILLTLPGLLGGIFVGWLAARMLVILLASRSPLTDITTRPNAIILAVNTGAAV
                     LVAVGAGLWPALRASRTDPSLDLKTGGHSVSARHSGGWAVSLQVAVCLTLVACATLVA
                     GVLSNLMAEHSGFRPQGAAMAKLDLQSLKLTDAEAAALENRLLNAVRQKPGVTAAGLI
                     DIPPFQGSFGASRMFSIDNRHAIHSDPSVFFAIASSGYFAAAGTGVIEGNAEASSSNI
                     SGCALSRSAAKFFFPDENAVGQLIYFSVAQQPDGTNLDLKNACRVAAVVDDANFVSLH
                     QPAPRMVYSIIRPEMPVKNFDQANHLVVRAGSDSLAVAALEQAGREVLPPLVEVKIQT
                     FTQLVHQDLARERMLVSLSGIFAILALLLTALGLYGLLMRDTVLRTREIGIRIAFGAP
                     KGAIVRAVSSRAGFQAAVGLVTGAAVSVLVIQAVRQLLELPQAGAFFVLAISAAIVSF
                     VILLAAMTPVSRALSIDPMEALRAE"
     gene            complement(5711..>6193)
                     /locus_tag="VFA02_03735"
     CDS             complement(5711..>6193)
                     /locus_tag="VFA02_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08988.1"
                     /translation="SEPCPYILRFDEDRPNPLYRKVCYTFAWNAVLSFALLNLAGLID
                     AVRSGSWHMMQIYRYGYFPLCGLVLLLGFFGVLPRVRRSTKGEGTERRYFYGAVWSVT
                     IAQAVLLLLWKTLPKTHATDVLKLVVYAGLLVILAAAAAFGLLPRTRPILPGEVMIAD
                     "
BASE COUNT         1429 a         1617 c         1785 g         1362 t
ORIGIN      
        1 gccatccctc aaagaaagcg aggcttacaa ggccatatcc cacaaagtgc ccggccttac
       61 ggatgtagaa atggatggtg gcccattgct ctggtgtgac cgggccaaaa agtttttccc
      121 aaacagggag cagccagcgg ctggtgttcc ccgaagagaa aaagccggtg gattcaatca
      181 cgatcaccag gaccgcaagc aaagccggaa tccagtgcag aagaagacga aaacgtgatt
      241 ttgccatgag ggcctcagta cctcggcacc gctggatcca taaccgaaga ccatgcgtct
      301 atgcctccgc gcagcgattg cacgtggtca aagccctgat tgcgcagcca gacggcaaca
      361 ttcatagagc gcactccgtg atggcagatt gtgacgatat gcgcatcggg atcaagctca
      421 tgatgggcac ggccggggac ttcgcccatg ggcatcagga tgctgccatc aatgcgggcc
      481 agcgcgtatt cctgtggttc acggacgtca agcaaaacag gtttttgagg gcctttcagc
      541 aactcggcca actgaaaggg tgagatctcg taatccagca tattgccaga atacaactct
      601 tcagaaaaac aaccctgcca tccaatgcat acaccggcaa gcgaagagta tgatgtttcc
      661 cgcagaggaa aggagccgtt ccatggtcat gctgcataac gggcaatgtg ggctctgtgt
      721 tcactttggc gagccgcatc ctgctactcc catgctgatt cagattcaca acagcaaaca
      781 ggcgcctgag acttttcttg atgactgcgg gcatccgaaa catgctccgc tgcatttgaa
      841 agtgagcgcc atgagtgggt gcgatggctt tgagccggcc aaggctgctt aaaggcgtcc
      901 ccgctttcaa aaccatgtgc ctgggtggcc agtccctgat atcctgcgcg caggatgctg
      961 ctagagctac gcgcggaaaa ctacgctgtt attgatcatg ccattgctgt gtttggtccc
     1021 ggattgaatc tgctcaccgg agagaccggg gcggggaagt ccattctggt agatgctctg
     1081 gccctgttga tgggcgagaa ggcgtcgtcc gatgtggtcc ggcatggcgc ggacaaggcc
     1141 gtggtgtcct gcgtcttcga gggtacaccc ggcgcagaat cagtgcttga ggccaacgga
     1201 attgattccg aagggcatga gatgattctg cggcgcgaaa tttatgcgaa tggtaaagga
     1261 cgtgtcttca tcaacaacca gcctgctact gtaactgcgc tgcggcagct tgctccggaa
     1321 ctggcgttgg tccatgcgca gagcgagacc ctgggggcct ttgatcaggc gcagcaacgt
     1381 gggctgctcg accgttttgg cgcaatttca actgagaatg tgggtctttt gtacgaaaaa
     1441 tggcgcgaaa ttcagcagaa gctggccgac ctggaacgcg atgagcagga ccggctgcgc
     1501 atggtagatc tctggagttt tcagcacagg gaaattgggg aaatcaagcc aactgaaggc
     1561 gaagatgagg cgcttgagac ggaacgccgt gtcctgtcga atgctgaaaa gctctacacc
     1621 gccgcaatga gtgctcacga gttgctttat gagggtggaa catcggctga gacggcatta
     1681 cgtgccgcgc tgaagcaatt ggaagaactg gcccgttacg acccacaatt tcaggacgct
     1741 atccagcaga tcagctcagc gcgcgctacg gtgcaggacg taagtgcaac tgtgcgcgat
     1801 tatgctgagg acattcaggc ttcgccagag cgccttgccg aaattgagga ccggcttgcc
     1861 gcgcttgacc ggctcaagcg caagtatggt cctactcttg ccggggtgat tgcatacggc
     1921 gaagaagttg ctgcgagact gaacgaagtt gagaaccgtg acgaaatcgt gaagtccctg
     1981 cgcgtggaat tgaaggccgc agaagacgag taccgtaacg ccgcacaatt gttaacaaac
     2041 ctgcgcggag aagcagcgaa aaaactggaa aagctggcgg aagcgcacat caatgacctc
     2101 gcaatgaaag tgcactttgc cgtaatggtc acgccggacg aagaccaggc ccactggacc
     2161 gcatatgggt gggacacagt cgaataccgc attgccacga atgcgggcga accgttgaag
     2221 ccactggacg agatcgcttc cggcggagag atgtcgcgcg tgctgctggc gctgaaagtc
     2281 agcgtggaag aaggcgcaaa taaggcgcag aagaagaaaa cacaggtgcc tcgcacgctg
     2341 gtattcgacg aaattgacat tggtattggc ggacgagctg ccgaggctgt tggtcagaaa
     2401 ctcaaggcgc tttctcgcgc acaacaggtc ctgtgcgtca cgcatcttcc gcagatcgcc
     2461 gccttcgcag accagcattt tcttatcgag aaaaaggagc aggggggccg cacaaagaca
     2521 tccgtccgcc tgcttgaaga ccatgagcga gtggaagagg tggcccgcat gttaagcggc
     2581 gcaaagctta cggacgcatc gttgaaacat gctgagcaga tggtaaaggc aagccggtag
     2641 tctgagcacg ggccggagtg tatatcgtat gcgtcaattg aaattacaaa aggattgttg
     2701 aggctgtgca ggaacaaagg acaaggcaca gcgttaaagt tcttctgctg taccacccca
     2761 atcctgcgga tgcacgtcag caactgattg cgaaaaggtc cacgagtaac ctgcaaaatc
     2821 tattgcgttg tactggcgct cgccatacga atgagtcact ggttcctgcg caatctgtgc
     2881 tccgtgattt ctggcgtgat tgcaatgagc atcgacatct tcaacgcgta ccatcactga
     2941 aaatcccacg cccaatgtag cggcgacttc gctcggacga atctccctca caactatcgc
     3001 tccatctccc gcatttaact gtatgcgatg gttgccgata cgcaggcgca cactaaaccc
     3061 gaacgcttca cacagccagg cagctgcctg gttcacatcc tggtatgcaa gcacagggat
     3121 tacagtcgca cgaggtatgg atctgttcga cagcattttt ccccctcact aaaacgtatc
     3181 tgcatattat tctttctgta tccaattgcg tgacaggaga ccgggatgca atggcccacg
     3241 tttgaatctc tctggctcga cacaaaatac gccttacgcc agatttttaa atcgcccgga
     3301 atttctctga ttgctgttct gacgctggcg tttggcattg gggcaaatac cgcgattttt
     3361 acgcttacgt gggccatcgt actccggagt cttcccgttc cgcaccctga acagcttgtc
     3421 cagtatggaa tgcgtaacgg aggaacactt atcggtttga gcggaccgga atacaacgca
     3481 ctgcggcaaa tgcaaaagac gtcagtggac ctgcttgcat ggacgagcga ccggccaagt
     3541 gtaagaacgg gcaatgacac gcgccgggaa gaagtgcagt ttctcacggg caatgcgttt
     3601 aagacgttag gattacgccc gtatctggga aatttcttta gtgatgcgca gcaaaacgct
     3661 gcgattctca gttatgagta ttggcagcag cagtttcatg gcagctctga cgtaatcggt
     3721 caatcgcttt ttgtgggcgg gcatccggtg gtcatcaccg gagtcatgcc agaggcattt
     3781 accgggttaa cggccaattt ccatccggct gtgtatctgc cgatgaaatt tggcaatgtt
     3841 ctttacggga aagattacac aaccgccccg ggacatcttg gattctatgc gatgggaagg
     3901 ctcaagcctg gagcgacgct gacccaggcc acagccgaag ccaaagccct ggagccaacg
     3961 attcggaagg aagccgaccc ttccggtatc tacctggggc aattctttca atcatttcat
     4021 cttgatgtgc agagcggacg cagcggcatt tcctgggtga agatggccta tgcgcgtccg
     4081 ctctttgtgc tggaactgct ggttatattt ctgattgtgc tgtgctgcct gaacgcggcg
     4141 ctggtgatgc tggcgcgagt cagcggacgc cagcaggaat ttgcagtacg aagcgcgctg
     4201 ggagcatcgc ggtcgaattt gatttctcag gttctgctgg agaccattct gctcactcta
     4261 cctggtcttt tgggcggcat ctttgtcggc tggctggcag cacgaatgct tgtcattctg
     4321 ctggccagca ggagtccttt aacagacatc accacgcggc ccaatgcaat tattcttgcg
     4381 gtaaataccg gggcggcagt gctggttgcc gtcggcgcag gactgtggcc ggcccttcgc
     4441 gccagcagga ctgatccatc tcttgatctg aaaacaggcg gacatagtgt gtcggcgcgc
     4501 cattccggag gatgggccgt cagcctgcag gtagctgtct gcctcacact ggtggcctgc
     4561 gcgacgcttg ttgctggtgt tctctcgaat ttgatggcag agcactccgg gtttcgtccg
     4621 cagggtgcgg ccatggccaa gctagattta cagagcctga aactgacgga tgccgaggcc
     4681 gctgctctgg aaaacagatt gttgaacgca gtacggcaaa agcccggggt gacggcggca
     4741 ggattgattg acataccgcc gtttcaaggc tcgttcggcg caagcagaat gttctcgata
     4801 gacaacaggc acgcgattca tagtgaccca agtgtatttt ttgctatagc atcgtcgggc
     4861 tacttcgctg ctgccggaac cggggtgatc gaaggaaatg cggaggcatc ttccagcaac
     4921 atttcaggat gcgctctcag ccgctctgct gcaaagttct tctttcctga tgaaaacgca
     4981 gtggggcaac tgatttactt ttctgttgcg caacaaccgg acggaaccaa tctcgattta
     5041 aaaaatgcct gccgtgttgc cgccgttgtg gacgacgcaa atttcgtctc tctgcatcag
     5101 cctgctccac gcatggtcta tagcatcatc cgtccggaga tgccggtgaa aaactttgat
     5161 caggcaaacc atctggttgt tcgcgctggc tctgattcac tggccgtggc tgcgcttgag
     5221 caggcgggcc gcgaagtgct gccaccgctt gttgaagtca aaatccagac ctttactcag
     5281 cttgttcatc aagaccttgc ccgcgagcgg atgcttgtta gtctttctgg aattttcgca
     5341 attctggcgc tgctgctgac cgcacttggt ctttatggat tgctgatgcg agacactgtt
     5401 ctgcgcactc gcgaaatcgg tattcgcatt gcttttggag cccctaaggg agcaatcgtc
     5461 cgggcagtta gcagccgtgc cgggtttcag gccgccgttg ggctagtgac tggagccgcc
     5521 gtttctgtat tggtgattca agctgtgcgc cagcttctgg aacttccgca ggcaggggca
     5581 ttttttgttc tggccatcag cgccgcgatc gtaagctttg tgatattgct ggcagccatg
     5641 actccggtca gcagggcatt gtctattgat ccgatggagg cgcttcgggc agagtagcgc
     5701 tattttgtca tcaatcggcg atcatcactt cgccgggaag gatcggtctg gtgcggggga
     5761 gaagcccaaa tgctgcggct gcggccaaaa tcacaagcag tccagcatat acaaccaact
     5821 tcaggacatc cgtggcatgg gttttgggca gggtcttcca gagcaggagc aggacggcct
     5881 gcgcgatggt gactgaccag actgctccat aaaaataacg gcgttccgtg ccctcgcctt
     5941 tggtggagcg gcgcacgcgt ggcagcacgc cgaagaatcc cagcagcaaa accagcccgc
     6001 aaagagggaa atagccatag cgataaatct gcatcatgtg ccatgatccg ctcctgacag
     6061 catctatcag cccggccaga ttcagcagcg cgaagctcaa cacggcattc caggcgaagg
     6121 tgtagcaaac tttgcggtag agcgggttcg gacggtcttc atcgaaacga agaatatagg
     6181 ggcaaggctc gga
//
LOCUS       DASRBV010000094         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_211011, whole genome shotgun sequence.
ACCESSION   DASRBV010000094 DASRBV010000000
VERSION     DASRBV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211011"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3054)
                     /locus_tag="VFA02_03740"
     CDS             complement(<1..3054)
                     /locus_tag="VFA02_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU08989.1"
                     /translation="MQGFIRTLLRYRNIVLILFLVSLAGGTIAFLHLDIEAYPDPSPP
                     MVEFITQNPSWSAEEMEQQVTIPIEAGLNGTPHLEQIRSISIFGLSDVKLYFTFDSDY
                     FTDRQEALNRLQSVNLPNNLQPQLSPESPIGEIYRYQLTGPGYTLNELKAVQDWFVVR
                     EIKQVPGIIDVATFGGTTRQYQAEVDPNKLLAYGVTMTQVLNAIQSSNANAGGNYLTL
                     GSQSVNVRGIGLLHSIADMENIVIAERNGVPVLLKDVADVKEGHQPRLGKVGYDDQPD
                     IVEGIVLLQKNEKSLPALAGLRQKLKELNEGNLLPPGMKIKTIYDRTNLINTTTKTVR
                     HVIITGLILVTLVLLSMLGDLRITAIAAITIPFAVLFSFAMMVLTDHSANLISIGAID
                     FGILVDASIVVLESVYRKISRRVPGEEVGELIMEGVGDAAKPVLFATLIILVAFIPLF
                     TMQGVPGKIFAPMSVTYGFALTGALIFALVFAPVLGSFAVPKEAKKEAREETWLSGFF
                     RRHYDNGLKRALKFGRTTWLIALAALIAAVVIFKFVGGEFMPALEEGNLWIRATLQQD
                     ISFQESEKLADKIRATLRTYPEVTQVISQMGRPDDGTDVSTFNNIEFLASLKPADEWR
                     ARFHGNKEELIAAIEHDFAQYPGVDFNFSQNIQDNVEEAMSGVKGENSLKLFGNDFDE
                     LTNLASRIEDVMAHVQGVTDIGVFKVTGQPSLMVSIDRSKAARYGLQAQDVNNVVQAA
                     VGGAPVTQIIDGDRRFDFAVRYSPQFRDTPEAISKILLPTPDGSQVQLGMIADVSIRN
                     GAFMIYHEGGRRYIPIKFSVRGRDLASTMQDLKQQLSDKVKLPSGYNYTWAGEFDSLQ
                     KEQRRLAYIIPVSLGVILVLLYIQFRTWRDAFIVLATLPFAAIGGILALFLTRTSFSI
                     SAAVGFTSLTGVATLGAVVFLSGIRRAQREEGFEHGLEKGCLDEMRPVVMACMAAGLG
                     LLPAAVSNGIGAQAQQPLARVVVGGMLTTVFAILFVMPLLVRRFSS"
     gene            complement(3056..>3848)
                     /locus_tag="VFA02_03745"
     CDS             complement(3056..>3848)
                     /locus_tag="VFA02_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU08990.1"
                     /translation="SSDAAQARSDFEKAKIEAARADLQLQRAKELLQHEVLAQKDYDD
                     LKALDDSDHAELERTRQALHNLGFSENDTSPNLPLRSPVSGVVLDVDTAPGEMQRSLD
                     NATNIATIANIDSIWVMGDLYPRDVDAVRRGQSVEVRVNGYPNEVRTGVIDNISDSVD
                     PTTLTLKVRVVLSNPGHKYKPQMYATISVANLKREAMVVPSTAVIRDGVKNYVFVATP
                     DHKYARRDVELGVTHQNDVEVTHGLEDGEMVVTTGAELLREAGVQ"
BASE COUNT          879 a         1136 c         1001 g          832 t
ORIGIN      
        1 ggacgagaac ctgcgcacaa gcagtggcat cacaaaaaga atcgcaaaca ccgtcgtcag
       61 cattcctccc acgaccacgc gcgctaaagg ttgctgcgcc tgcgccccaa ttccgttgga
      121 cacagcagca ggcagaagcc ccagcccagc agccatgcag gccatcacaa caggacgcat
      181 ctcgtccaga cagccttttt ccagaccatg ctcaaagcct tcttcccgtt gcgcgcgccg
      241 gatacctgaa agaaacacga ctgctcccag cgtggccacg cctgtgagcg aggtgaagcc
      301 gactgccgca gaaatactga acgacgtgcg cgtaagaaag agcgcaagaa tgccgccaat
      361 cgcggcgaaa ggaagcgtcg ccagcacaat aaaggcatcg cgccaggtgc gaaactgaat
      421 gtaaagcagc accagaatca cgccgagact gaccggaatg atatacgcca gacggcgctg
      481 ttctttctgc agactgtcga attcgcccgc ccaggtatag ttatatccgc tgggcagctt
      541 cactttgtct gaaagctgct gtttcaggtc ctgcatcgtg ctggccaggt cgcgtccccg
      601 cacgctgaac ttgatgggaa tgtatcgccg tccgccttcg tggtaaatca taaaagcgcc
      661 attgcggatg ctgacatcgg caatcattcc aagctgtacc tggctgccat cgggcgtggg
      721 cagcagaatt ttgctgatgg cctccggcgt atcgcggaat tggggggagt agcgcacggc
      781 aaaatcaaaa cgacggtccc catcaatgat ctgcgtcacc ggagcgccgc ccactgcggc
      841 ctgcacaacg ttgttcacat cctgcgcctg aagaccatac cgcgcggcct tgctgcggtc
      901 aatcgaaacc atcaggctgg gttgccctgt cactttaaac acaccgatgt cagtgacgcc
      961 ctgcacatgg gccatcacat cttcaatccg gctggcaagg ttcgtcagct cgtcaaaatc
     1021 gttgccgaag agcttgagcg agttctcgcc ctttacaccg gacatggcct cttccacgtt
     1081 gtcctgaatg ttctgggaga agttgaagtc cacgcccgga tactgtgcaa aatcatgctc
     1141 gatcgccgca atcagctctt ctttgttgcc atggaaccgc gcccgccact cgtccgcagg
     1201 cttcagactg gcaagaaatt caatgttatt gaaggtgctg acgtccgtcc cgtcatccgg
     1261 ccggcccatt tgcgagatga cctgagtcac ttcaggatac gtgcgcaggg tggcgcggat
     1321 tttgtcagcc agtttttctg attcctgaaa ggagatgtcc tgctgcaacg tggcacgtat
     1381 ccatagattg ccctcttcca gcgcaggcat aaattctccg ccgacaaact taaaaatcac
     1441 aaccgcggca atcaacgcag ccagagcaat cagccatgtg gttctgccga atttcaaagc
     1501 acgcttcagc ccattgtcat aatgacggcg aaagaatcca cttaaccatg tttcttccct
     1561 ggcctctttc tttgcctctt tggggacggc aaacgagccg agcacggggg caaaaacaag
     1621 agcaaaaatg agcgccccgg tcagggcaaa tccataggtc acagacatgg gcgcaaaaat
     1681 cttaccggga acgccctgca tggtaaacag cggaataaac gcgacaagaa taatgagcgt
     1741 agcaaaaaga acaggcttgg ccgcatcgcc cacgccttcc ataatcagct cgcccacttc
     1801 ttcacccggc acgcggcgcg aaattttccg atagacgctc tcaagcacca cgatggaagc
     1861 gtctaccaga atgccgaagt caatggcccc gatggaaatg aggttcgccg aatggtcggt
     1921 gagcaccatc atggcaaatg agaagagcac cgcaaacgga atcgtaatcg ctgcgattgc
     1981 agtaatacga aggtctccca acatgctcag cagcaccagc gtcaccaata tcaatcctgt
     2041 gatgatgacg tgccggacgg tcttcgtcgt ggtgttgatg aggtttgtgc ggtcgtagat
     2101 ggtcttgatc ttcatgcccg gcggcagaag attgccttca ttcagctctt tgagtttctg
     2161 gcgcagtccg gcgagtgcgg gcagtgattt ctcgtttttc tgcagaagca ctatgccttc
     2221 cacaatgtcc ggctggtcat cgtatccaac cttgcccaga cgcggttgat ggccttcctt
     2281 cacatccgcc acgtccttca gcagaacagg cacaccattg cgttcggcaa tcacgatgtt
     2341 ctccatgtcc gcaatggaat gcagcaggcc gatgccgcgg acgttcacac tctggctgcc
     2401 aagtgtgaga tagttgcccc cggcatttgc attgctgctc tggattgcat tgaggacctg
     2461 cgtcatcgtc acgccatacg caagcaattt gttcgggtct acttctgcct gatattgccg
     2521 cgtcgtaccg ccaaacgtgg ccacgtcaat aatgccaggc acctgcttga tctcacgcac
     2581 tacaaaccag tcctggaccg ccttcagttc attgagcgtg taccccggcc ccgtaagttg
     2641 ataacggtag atttccccaa tcggagactc gggcgaaagc tgcggctgca aattattcgg
     2701 caggttgacc gactgcagac ggttcagcgc ctcctgccgg tcggtgaagt agtcgctgtc
     2761 aaaggtgaaa tagagcttca catcgctcag accaaagatg ctgatcgagc ggatctgctc
     2821 caggtgcggc gtaccattca gtcccgcttc aatggggatc gtcacctgct gctccatctc
     2881 ctcggcagac cacgatgggt tttgcgtgat gaactcaacc atcgggggcg aagggtctgg
     2941 ataggcctcg atgtcaaggt gaaggaaggc aatcgtcccg cccgccagtg agactagaaa
     3001 cagaatgagg acgatattgc ggtagcgcag aagggtccgg ataaagccct gcattttatt
     3061 gcactcctgc ttcgcgcaga agctccgcgc ctgtggtcac aaccatttcg ccatcttcca
     3121 gaccgtgcgt gacttccaca tcgttctggt gtgtcactcc cagttcgaca tcgcgccgcg
     3181 cgtatttgtg gtcaggcgtt gcgacaaaga cataattttt cacgccatca cggatgacgg
     3241 ccgttgatgg aacaaccatc gcctcgcgtt tgaggttcgc aactgaaatc gtcgcgtaca
     3301 tctgcggctt gtacttgtgc ccgggattcg acaacaccac gcgtactttc agcgtgagcg
     3361 tcgttggatc aaccgaatca gaaatgttat ctatcacccc cgtgcgcact tcattcggat
     3421 atccattgac ccgcacttcg acggattgcc cgcgccgcac agcatccaca tcacgcggat
     3481 acaagtctcc catgacccag atcgaatcaa tattcgcaat cgtggcaatg ttcgtggcat
     3541 tgtccagcga acgctgcatc tcaccagggg ctgtatccac atcgagcacc acgccgctga
     3601 cgggcgaacg cagcggaagg ttcggcgatg tgtcattttc tgagaagccc agattgtgca
     3661 gcgcctgacg tgtgcgctca agttcagcgt ggtcggaatc gtccagcgcc ttcagatcat
     3721 cgtagtcttt ctgggccagc acttcgtgct gtaggagttc ctttgcgcgc tgcaattgca
     3781 ggtctgcgcg cgcagcttca atcttcgcct tctcaaaatc agaacgcgcc tgcgccgcat
     3841 cactgctc
//
LOCUS       DASRBV010000095        20466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_410315, whole genome shotgun sequence.
ACCESSION   DASRBV010000095 DASRBV010000000
VERSION     DASRBV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 20466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20466
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410315"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..1501
                     /locus_tag="VFA02_03750"
     CDS             497..1501
                     /locus_tag="VFA02_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU08991.1"
                     /translation="MVRMTVLASGSKGNSTIVSSSRTRILVDAGLSCRELFKRMKSVN
                     EDPDTLDAILVTHEHQDHVQGLSVTARRLGIPVYFTEATHRAWMRWMTPRKRLTYAEW
                     LEQRKQEAEKKKAEAEAKGIEPDADTANPFYREQELAECAAEEEELKSESKEEQSLDA
                     KKDADPCALPAVEYFRAGSQFSIGDITINAFTIPHDAIDPVGFVFQAEGVRIGIATDL
                     GYMPPNVGLQLRGCDVLMLESNHDLEMLRDGPYPWAVKQRVMSRVGHLSNDAAASFLE
                     SHYDGHASYIVLAHLSESNNLPELARLSAERALRDRMTLLGNKLVLAEQNAAIEPILL
                     "
     gene            1704..2072
                     /locus_tag="VFA02_03755"
     CDS             1704..2072
                     /locus_tag="VFA02_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU08992.1"
                     /translation="MRTDYEQHLEECQHCRSRQRLHRTVDVVLIGVATFSSMVFLLAL
                     AVIHRIEPLRNWAVVNLHLRQMEVVLTLQAAAVFGLLISILAWILVAIATPAPMYLTH
                     MALAQARGLQNRDRTTSKAA"
     gene            complement(2117..2491)
                     /locus_tag="VFA02_03760"
     CDS             complement(2117..2491)
                     /locus_tag="VFA02_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZU08993.1"
                     /translation="MTGVRMDKWLWAARFFKTRALASRACELGRISSNGQQAKPAREV
                     RVGDLLQIRNDSGEFQVEVLQLSEVRGPAAVAQTLYRETEASRELRQKLAEERKTMPQ
                     FDFLEGRPSKRDRRQLARLRGR"
     gene            complement(2500..3825)
                     /locus_tag="VFA02_03765"
     CDS             complement(2500..3825)
                     /locus_tag="VFA02_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU08994.1"
                     /translation="MFTRREFAKGLASTTAGLAVATTAKSYSQIMGANERINFAVAGL
                     NSRAYAHFDGLHANRDSARITHVADVDTNILGKYAGFVEKVTGSAPKADQDFRKTLES
                     KDVDALTIATPDHWHTPMAIMALEAGKHVYVEKPCSHNVHECELLVAAQKKYNKLVQM
                     GNQQRSSPHTIEIIEKIQSGLIGRAYFAKAWYSNTRKSMGTGKVVPVPPNLDWDLWQG
                     PAPRRPYKDNIHPYNWHWLRIYGTGEALNNGTHEVDVCRWALGVKWPERITSSGGRYQ
                     FKDDWQFYDTLVTSFEYSDKMISWENMSCNGMPLYDRDRGSAIHGTEGTVIVDRDGYE
                     VRDLKGKLINQYRVPKAGKTSSADLVGSDSMTQLHFKNLIDGIRTGAKLHSPIDEVYA
                     SVSILLMSNIAWEVQRELKLDSSTSAFIGDEEATRLRRRTYEKGWEPRV"
     gene            complement(4272..5390)
                     /locus_tag="VFA02_03770"
     CDS             complement(4272..5390)
                     /locus_tag="VFA02_03770"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HZU08995.1"
                     /translation="MLRRLFPIALALFSLAQLSAQKAAPPSSQQQQAFLPSDFAGWHA
                     SGPVQQSQSPEAADQANAPVLHEYGFAEFASANYVQPDNKLSVRAIRFEDATGAYGAF
                     TFYRRPGMRKEDIGAEGAFDGSHVLFWKGATLVDATFDHLTAMSAAQLRELAQALPNI
                     SGPSSIAPPLPKYLPPASLDAGSVRYALGPESYRQGGGVLPPAQVGFNRDAEAVTAHY
                     STTHGDGTLTLLMYPTPQIAIERTKAIQNFLKAGSTSQWPQPLSESLADALQVRRSGP
                     MVAVTSGNFSASQAHTLLEEVNYSADVTWDRPQGYVSESSKTARLLLGIAALTGIIGG
                     TAIILGIFFGGGRALVRRLRGKPASSLSDEQFIRLKLR"
     gene            complement(5441..6169)
                     /locus_tag="VFA02_03775"
     CDS             complement(5441..6169)
                     /locus_tag="VFA02_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU08996.1"
                     /translation="MTARLFAFILAAVFSFPLYATAQQTDVERFDLYSGFTFFNSPSL
                     NLAEHGYHLQAGYNATRILTLGFDYSIVTGHNSLTPNLLTDSLQLQVATALKDLALAG
                     LLPPGYQLSVPTDSTTQTFAAGTQFSYRHFVPVTLFVRPDLGAIREEATPHPRDPIAA
                     LIVQQLAPSGHKTSWTSFYGFGGGLDWNVTRHLALRFQADQVYNHLFNDLLKDSRWTT
                     RASAGLAFRFGGNILEGKAARPKP"
     gene            complement(6264..7217)
                     /locus_tag="VFA02_03780"
     CDS             complement(6264..7217)
                     /locus_tag="VFA02_03780"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZU08997.1"
                     /translation="MQVFRALSEVPANYGPTVIAIGNFDGVHCGHRWIIQHARERAGE
                     LGAKRIAVTFDPHPVRVLRPEIAPRLITPMAERLRLLAETGLDATVVLPFTEEFSHLS
                     AEDFARNVLRDGLNAVEVHEGDNFRFGHQAQAGITELMALGRVLGFAVTAHHTRIVRG
                     FPVSSSKLRELIAAGNMTAARALLGRPFSIRSTPARGRGIGSKLTVPTINLAQYDELL
                     PADGVYITRMKIGDGPVFDSVTNIGVRPTFGDASFTVETHLLDFRPIELTGTTSLELT
                     FLHRIRPEMRFPSPETLKEQILRDVKQARRYFHLAKLFKDN"
     gene            complement(7220..7999)
                     /locus_tag="VFA02_03785"
     CDS             complement(7220..7999)
                     /locus_tag="VFA02_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU08998.1"
                     /translation="MQATLTILGSGTSMGVPTLGCECHVCTSPDSRDRRTRPSAAVQW
                     GGHCVLIDTGPDFRTQALRENIRHVDAVFYTHSHADHILGLDDLRPLSFHHPSGHIPL
                     YADDFSARIIEKVFDYTFSAESRYPTRARVKMNRLERTESVTIQNAIFQRVPLIHGEQ
                     EAAGFRFGSAAYLTDMNRIPDGSLPLLQNLDVVIMDALRKKPHPSHANIDEALHWVEV
                     LKPRRVWFTHMSHDLLHAETEDELPPHVRLAYDGLRIPFEL"
     gene            complement(8109..8696)
                     /locus_tag="VFA02_03790"
     CDS             complement(8109..8696)
                     /locus_tag="VFA02_03790"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZU08999.1"
                     /translation="MENKPPAFTVTDRRKFTPEGERRDDPAQSEQQEKPAENGPRVIT
                     MPPPKQEAPRAEETPQPATEEHAGPTAQESAAQHEAYQQSTREIDDMLRQANPGMGAP
                     QEVAFEHVIQSIYLSALVAMGAGTEPGQQPRIDIVGARQSIDMLAVLEQKTKGNLTEK
                     EQRLLQNALFDLRMMYVEITNAISKSAQQPPLAKK"
     gene            complement(8711..9790)
                     /locus_tag="VFA02_03795"
     CDS             complement(8711..9790)
                     /locus_tag="VFA02_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZU09000.1"
                     /translation="MGQTPPLVENERTPLASLHVASDLQMHRGRLTPHVFEDCTIELG
                     HLMHSAAVYDLGYLTRIRITGKDRVRWLNGMVTNNTRDMAEGSGLYTFVLNAQGRIQG
                     DGYIFRSPDHYLFETDRLQLPRLLAHLDHFIIMDDVDLQELDAANTTLGLAGPEAPAI
                     LESLGIDIPEKMTFAQTAIQGIPVTLVSAYEVLVPRFELWLASEHAGEMWRILTEAGA
                     KPAGFAALNSLRIFEGVPEYGVDITDRALPQETNQMRALNFSKGCYLGQEIVERIRSR
                     ATVHRTLKQFDLHGDLPQSGAELHVEGDDKPVGEITSASHYALPGFTQTLALGFVRIE
                     VLERKAAITYAGGTATPLEGPLALP"
     gene            9823..11337
                     /locus_tag="VFA02_03800"
     CDS             9823..11337
                     /locus_tag="VFA02_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09001.1"
                     /translation="MKTAGRLVLVVMVAAGMCLGQTTDNAAAQKVAPHETHITPSQAK
                     ELFHSVGVILKFASEDTDLPIRHDVKRKLTTREDVEKYLIRKMDDDKDAKRMERSEIV
                     LKKFGLLDRDFQLRPFLLALLKEQIEAYYDSKTKTVNLLDWATPETQKPVLAHELTHA
                     LQDQHVDLDKWEDVTSEDLSHNAKQDNEHIQTDEVDTAREAVLEGQAMAVYVDYSLAP
                     SGKSLLNAPDLDTDDLGGDTSDSPVLSRAPLVLKESLLFPYQDGLHFEQVLLKNEGKD
                     GAFAGVLDRPPASSYEVMNPRAYLRKQKAPVLSMPDVHPLLDADYDPYDVGVMGQLDV
                     RMLSELFGGEQLSKVLTPAWDGGIYYAVQSKKAKTGAEKDSTASIGLLYLSRWKSPEA
                     ARAFAKMYADELSKKYSGVKRDPDAEGDPTEQVYQTSEGPALIVTKDNMVFTSESFDL
                     PLARKLAFLMMGAQEGGGSLEAAKFKAPGHPSHELTTSMVRFFTGCGMVRAAVR"
     gene            complement(11393..13072)
                     /locus_tag="VFA02_03805"
     CDS             complement(11393..13072)
                     /locus_tag="VFA02_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N4-asparagine amidase"
                     /protein_id="HZU09002.1"
                     /translation="MFFPVRQILAAALFGAAALSFGLAQTPVVGSSNTATADPPVRHP
                     NTTPCTVTLFSDFTFDDFSPRNFTYIPPANCPGPWAKVVLVADFNENAGRQFDRTAKM
                     FLNGVNIYFGTTPEPRKTVAPTWHIERDLTDYSALFTQPQSGDIHLDNVVDSTYTGVI
                     HGSAQLLFYPATFFSPAPHTADQVLALPTGATDSTYLNTPSNQLSQTVTLPTNVERAY
                     LDVLAQGQSTDEFWYTCVPNADTATLQSCGSTAFRETEITIDGQPAGVAPVYPWIFTG
                     GIDPYLWAPIPGVQTLNFVPYRVDLTPFAGLLSNGKPHTIALSVFNADSYFAVTANLL
                     VFEDHGGKSVTGAVIENTLAAAPAPVIQDQGVSSSGGTVDTSNLRNYRISGWVNTSHG
                     RIETTVEQNITFTNNQQFTISNTQYEQAIVQGTEISSRTITRDGWYVTEKHKKLSYPL
                     SLAYNYTTNTDGSSQQVTTVQQGFHEHETLLLNGFPLYQSETSNAVNTADTLQFGANG
                     SLTGTTDRSSSQKYFTRDSTGYCYSRELRSAAGLLTAVEDGKGCPKAWDGR"
     gene            13234..14103
                     /gene="mtnP"
                     /locus_tag="VFA02_03810"
     CDS             13234..14103
                     /gene="mtnP"
                     /locus_tag="VFA02_03810"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZU09003.1"
                     /translation="MQQAEIGIIGGSGLYAMPGLTNTREERVETPFGEPSDAFVLGEL
                     EGRRVAFLARHGRGHKLLPTELNFRANIYALKKLGVERIVSVSAVGSLKEEHKPTDFV
                     VPDQFIDRTFHRESTFFGDGLVAHVAFGDPVCATVAKAITDGCSASGVVGKQGGTYVC
                     MEGPQFSTRAESNLYRSWGADVIGMTNLQEAKLAREAEICYATMAMVTDYDCWHEGHD
                     DVTVEQIIAVLNQNAANAAKVVRAAVAAMPKERKCACATALQYAIITNPAAVPPETRK
                     RLDLLVGKYLKKD"
     gene            14109..15044
                     /locus_tag="VFA02_03815"
     CDS             14109..15044
                     /locus_tag="VFA02_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZU09004.1"
                     /translation="MSILVVGSVAFDSIETPSGRAERCLGGAATYFSLAASYFTDVRV
                     IAVVGEDFTAKEEDVLKRRDIDTQGLEHVAGKSFFWRGSYMKDLNEAQTQETQLNVFE
                     SFSPKIPAAYTDSEFLFLANIDPVLQDTVRRQMPKVRMVCGDTMNYWITGHRENLERV
                     LKGLDGLLINDGEAKLLANDNNLVCAAQKILAMGPKSLVVKHGEYGATAFFSNRLFPG
                     ETKHVSLPFRAPALPLSEVVDPTGAGDSFAGGFFGHLASQKEVTPASFKRAMFYGGVM
                     GSFAVERFGTERLQSLTREEIDQRFALFRELSHLD"
     gene            15067..16665
                     /locus_tag="VFA02_03820"
     CDS             15067..16665
                     /locus_tag="VFA02_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09005.1"
                     /translation="MSHPSHSKEWETESLRSETWLVACVALAVSLMALAYCSPRGMLL
                     LYGDAVAHLHIARRVFDSLDPGYKQLGSVWLPLSHILLIPFVQKMSWWQTGLAGAWPS
                     MACYVLGCAGIYRLARIWLRPAAAWIAVAFFALNPGLLYMQTTAMTEPLFLAEMVWSV
                     LLLAGYARALKSSDAVRASRLLIGLGFVLAGAVYTRYDGWIFACIAWWIALWNLRRHW
                     SSRAGGAFILFTAILVTAPLLWMAYNARQFGDPLDFMRGPYSAKAIEERTAAPGAPHY
                     PGWHSVRVAALYFLKAAELGAAPVRLANLMLWLSVAGTIAAFWKWRGQSITSALLLWV
                     PLPFYAYSIAYGSVPIFIPLWWPHSWYNTRYGMEMLPAFALFPAFLVAWAARQWPRAR
                     RWVIAVALALIVADSITLMRNRPIVLAEAIANSRTRIPYEESLAEALKSLPQQGMILM
                     YTSEHIGALQRAGIPLKRTINETDYYQWPAALQHPARAASIVVATDHDAVAKAIAAHP
                     EGLMLVDVVCSTGQPCARIYLSKW"
     gene            16684..17397
                     /locus_tag="VFA02_03825"
     CDS             16684..17397
                     /locus_tag="VFA02_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZU09006.1"
                     /translation="MMIEAPGHMLSFDALQEAILTGGAAGRLLLAAFLGGVIGIDREY
                     HHKPSGVRTNLLICFGAALFTFLSSVVAGEGSTNKGQIASNIVQGIGFLGAGLILHNR
                     DRVSGLTSAATVWAVASIGMACGAGLYIPAVFSAVLVLVALEGVGLLERKANLKLYSV
                     IYEARGRDPVKMSMTILHAMDRMKRRLGHLEQENIGDLNRICFTVYATRKGHDQLQAT
                     LNASPAIDEIHIFPNVEDD"
     gene            17394..18200
                     /gene="dapF"
                     /locus_tag="VFA02_03830"
     CDS             17394..18200
                     /gene="dapF"
                     /locus_tag="VFA02_03830"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZU09007.1"
                     /translation="MIPFVKAHACGNDFLVVEAKHLGAEKQRETAIRLCARNTGVGAD
                     GVEYLEWTGERSGRIRLFNADGSIAEISGNGTRCVAAWMAHEKGLQRGDVLTLETDAG
                     PRECRIVLNEDGRYQIASRMGVPEISRRGVDLNGTQIEGVAVSTGNPHFVIFVHDGTF
                     TALNRSWQEIGHAICFHPDFPQQTNVEFVHILNTNEIEIRIFERGVGPTASSGTGTCA
                     SAVAAISQRGAAKHLLVRAPGGEQRVEWDGEGSEILLTGPAQLIAQGEAF"
     gene            18201..19127
                     /locus_tag="VFA02_03835"
     CDS             18201..19127
                     /locus_tag="VFA02_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZU09008.1"
                     /translation="MLKPPAVAPDARVAVIAPASSSKPERLARGIEALRARGYDAEFG
                     QFAQGRLAPYFSGTAEERLADFHAAFSDPEIQAIFCTRGGYGSNYLLEDIDLDLIREN
                     PKPLFGYSDMTAVQTWLLDQTGLVAFHGPMVAADFAVSGGVHGPSLDAALDGDLISVS
                     AAEGLRILRPGRARGVMYGGCLSILTSTLGTRFAPQTEGKLLFLEDVGAKPYQIDRML
                     RQMVLAGKFDGVTGFIFGEMLECASPGAPPDLLEQVILRVLDWFDGPVAIGLRSGHVS
                     HSNITLPFGIEAELILEDAPELRYLEPAVENE"
     gene            19172..>20466
                     /gene="mpl"
                     /locus_tag="VFA02_03840"
     CDS             19172..>20466
                     /gene="mpl"
                     /locus_tag="VFA02_03840"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZU09009.1"
                     /translation="MQSKHIHLIGICGTAMASLAGLLQLKGHRVTGSDQAAYPPMSDL
                     LCSLRIPVAEPFSEENLKPHPDLVIIGNAISRGNVELEYVLDQRIPFTSLAALMHHEF
                     LRGRESLVVAGTHGKTTTTSMLAWMYEVAKLAPSFLIGGVAENFGTSFQLHDSRPFIM
                     EGDEYDTAFFDKGPKFMHYFPDALILTHVEFDHADIYRDLDQVKTAFKRLVNLVPRRG
                     RIVAFDGAANVSECVARAFCKVERYGFAPDSSWRITDLHFAGGQTRWKVLRDGKQWAD
                     LALNMAGEHNALNAMAAAALAAGQGVPMSAIQEALATFKSVKRRLEVRAVVDGVTIID
                     DFAHHPTAIRETLRALRTAYPDARLWAVLEPRSNTLRRNVFEAELVDSLSRADHIVLS
                     GVFKQESIPENERLHPETIVAALKQSGKSAALFANADAIV"
BASE COUNT         4349 a         5632 c         6017 g         4468 t
ORIGIN      
        1 ccggcttgtt gtccatgtgc cgatctcctg tgctggtttt tttagtttgt tactgcgtta
       61 aaaactcatg ccaaatgcca gaaggcaaat gcttctatgc gttaagtaaa cacaatacga
      121 ggtctgcaac aatcatgcaa actccacaac ggcctacagg tgcttgtaca caaaacacct
      181 taggcaatct tagatgcaaa acttcgctct gagatgcctg gacctattac tgtgaatccg
      241 tatacccgat agtggtcttt tcggtccttt cgtttcaaga aagtggactg accagagaaa
      301 ctgccccatc atctgcaagg agcaatctgg ggtgcgattc cgtccacccg ttttccggat
      361 cgaagggagc gcgccgcgga gtggatggag ctttgccgtt aaagatacag ataagctcag
      421 gaggcatgtt ccaaccaggt tcctgtctct gatttggccc gtttcttcca caccggttgg
      481 cagtatcctg aagaacatgg tcaggatgac ggtgctcgca agtggttcca aaggcaacag
      541 caccatcgtc tccagttcca gaacgcgcat tctcgtagac gccggacttt cctgccgcga
      601 gcttttcaag cgaatgaagt cggtgaatga agacccggat accctggatg ctattcttgt
      661 gacccacgaa caccaggacc acgtgcaggg cctttccgtg actgctcgca ggctgggcat
      721 tccggtttac ttcaccgagg caacgcatcg ggcatggatg cgctggatga ccccgcgaaa
      781 gcgccttacc tatgccgaat ggctggagca gcgtaaacag gaagctgaaa agaagaaggc
      841 cgaagccgaa gcaaagggca tagaaccgga cgcagacacc gccaacccct tttatcgcga
      901 acaggaactc gccgaatgtg ccgccgagga agaagagctg aagtcggaat cgaaagaaga
      961 acaaagttta gacgcaaaaa aggatgctga tccctgcgcc cttcctgctg tcgaatactt
     1021 tcgcgcaggc agccagtttt cgatagggga cattaccatc aacgccttca ctattccaca
     1081 cgacgccatt gaccccgtcg gctttgtctt tcaggctgaa ggtgtgcgca ttggcattgc
     1141 taccgatctc ggctacatgc cgccaaatgt cggcctgcaa ctgcgcgggt gcgatgttct
     1201 gatgcttgaa tccaaccatg acctggagat gctgcgcgac gggccatatc cgtgggcggt
     1261 caagcagagg gtcatgtccc gggttgggca cctgtcaaat gatgctgctg ccagttttct
     1321 ggaatcgcat tacgacggcc acgcgtctta tattgtgttg gcgcatttat cggagagcaa
     1381 caatctgccg gagcttgcgc ggctctcggc ggaacgggcc cttcgagacc gtatgacatt
     1441 gctgggcaac aagttagtgc tggcagaaca aaatgcggca atagagccta ttcttttgta
     1501 agcggaagtc atctatacta aagagagttt tcccacggcc catttttcca gcacccaggc
     1561 ttgttccagc agccgagcag gggccgtgat cgccagcaga cgaatgaaag cggagtgaat
     1621 gtactatgac atcctgcact gatccagtag ttggcgacat attatccagt tggcgatacg
     1681 atatctccgg tatttctccg gaaatgcgca ctgactatga gcagcacctg gaagaatgcc
     1741 agcattgccg ctccaggcag cgcctgcacc gcaccgttga cgtggtgctg attggcgtgg
     1801 ccaccttctc cagcatggta ttcctgctcg cgctggcggt cattcatcgc attgagccgc
     1861 tgcggaactg ggctgtcgtc aatctccatc ttcgccagat ggaagtagtc ctcacgctcc
     1921 aggctgccgc cgtgtttggt cttcttattt ccatcctggc ctggattctg gtcgccattg
     1981 caacgccagc cccgatgtac ctcacgcata tggccctggc gcaggcacgc ggcctgcaga
     2041 accgcgatag aacaacaagc aaagctgcct gatacacagg gaaagcaagg cccgtcgcct
     2101 gacgggcctt ctgcttttac ctgccgcgaa gccgcgcaag ctgtcgccgg tcgcgcttgg
     2161 acggccttcc ttcaaggaaa tcaaactggg gcatggtctt tcgctcttct gccagcttct
     2221 gccgaagctc ccggctggct tccgtttcgc ggtaaagcgt ctgcgcgacg gccgcagggc
     2281 cgcgcacctc gctcaattgc aggacttcca cctgaaactc gccgctgtca tttctgattt
     2341 gcagcagatc gcccacgcgc acttcacggg ctggtttggc ctgttgtcca ttggaactga
     2401 tgcggcccag ctcacaggcc cgcgaggcga gcgcgcgcgt cttgaagaag cgggcggccc
     2461 aaagccattt gtccatgcga acgccagtca tgaagtctct tacacccgcg gctcccagcc
     2521 tttttcgtag gtgcggcggc gcagacgggt ggcttcctcg tcgccaatga aggcactggt
     2581 ggaagaatcc agtttcagct cgcgctgcac ctcccaggca atgttcgaca tgagcagaat
     2641 ggagaccgaa gcatatacct catcaatagg agaatggagc tttgcgcctg tccggatgcc
     2701 gtcaatcagg tttttgaaat gcagttgggt cattgagtcg ctaccgacaa gatcagcgct
     2761 cgaagttttg cctgcctttg gtacgcggta ttggttgatg agtttgcctt tcaggtcgcg
     2821 gacttcatat ccatcgcggt ctacgatgac tgtgccctcg gtgccgtgga tggccgagcc
     2881 acggtcgcgg tcatacaatg gcattccgtt gcaggacatg ttttcccagg agatcatctt
     2941 gtccgaatac tcaaagcttg tcaccagcgt gtcataaaac tgccagtcat ctttgaactg
     3001 atatcggccc ccggaagagg taatgcgttc gggccacttc acaccgagcg cccagcggca
     3061 gacgtccact tcgtgcgttc cgttgttcag tgcttcgccc gttccgtaaa ttcgcagcca
     3121 gtgccagttg tagggatgga tgttgtcttt gtacggacgt cgcggagcgg ggccttgcca
     3181 taagtcccag tcgaggtttg gcgggacagg aaccacttta cccgtcccca tagactttcg
     3241 cgtattgctg taccacgcct tggcgaaata ggcgcggcca atcaagccgg attggatttt
     3301 ttcaatgatc tcaatcgtgt gcggtgagga gcgctgctga ttgcccatct gcaccagctt
     3361 gttgtatttc ttctgtgccg ccacaagcag ctcgcattca tgcacgttat ggctgcacgg
     3421 cttctccaca tagacgtgct tgcccgcctc cagggccatg atggccattg gagtatgcca
     3481 gtggtcgggt gtggcgatgg tcagcgcgtc tacatctttc gattcgagtg tcttgcggaa
     3541 gtcctggtcg gcctttggcg cagagccggt caccttctcg acgaagcccg cgtacttacc
     3601 caggatgttt gtgtctacat cggccacgtg tgtaatacgc gccgaatcgc ggttggcatg
     3661 gaggccgtca aagtgcgcat aggcgcggct gttcagtccg gcaacagcaa aattgatgcg
     3721 ctcgtttgcg cccatgatct gcgaataact ttttgcagtt gttgcaacgg caagtccggc
     3781 agtagtagag gcgagtcctt tggcaaattc gcggcgtgtg aacatggttc tccaggagaa
     3841 agttgaattt taaacgttct cggttttgaa aagcgtggcg cccgaaatgc ggcacgaaat
     3901 ttttcgcaag aacccagcgt atcaggatag agacatcgca tgtaccgtca ggcgaaatga
     3961 aggaagccgc ccggcaacag ggcggcttcc ttcgttaggg agaatagttc caacggaggc
     4021 aaaaccatca gaactttctg gcgaaatact atgggtgcgc cggaggggtg tcaaggcaaa
     4081 atccaagaaa catttaatta ttttgaaatc aatagcttgc aatatccatc cgtttcaggt
     4141 tacgaacaga gcttattcgc cttttcttcg tttctagtcg gagaaatata tgtaaagtca
     4201 tttaattttt tgcacttatg aaagtttgca cacatttatg tgcacccgag atgaaacaac
     4261 gagttttgca cctacctcag cttcagccgg ataaattgct catcactcaa cgaagaagct
     4321 ggttttccgc gaagacggcg gaccagtgcg cgtcctcccc caaagaatat gccgagaatg
     4381 atagctgtgc ctccaatgat tcctgtcagt gcagcgatgc cgagaagcag ccgcgcagtc
     4441 ttcgatgatt cgctcacgta accctgaggg cggtcccagg tcacatctgc gctgtagttc
     4501 acctcttcca gcagcgtgtg cgcctggctt gcagagaaat ttccactcgt gacggcaacc
     4561 atcggaccac tgcgccggac ctgcaaggca tctgcaaggc tctcggaaag cggctgcggc
     4621 cactgtgacg tgctgcccgc tttcaggaag ttctggatgg cctttgtgcg ttctatggcg
     4681 atttgcggtg tggggtacat gagcagagtc agcgtgccgt cgccgtgagt cgtggagtag
     4741 tgcgcagtga cggcctctgc atcgcgatta aagcccacct gcgcaggtgg cagcactcct
     4801 ccaccttgcc ggtaagattc cggacccaga gcataacgaa cgctgcctgc atccagcgac
     4861 gcgggcggaa ggtatttcgg aagcggcggc gcaatcgagc tggggccgga gatgttgggc
     4921 agcgcctgcg caagctcgcg caattgcgct gcggacatgg ccgtcaggtg gtcgaaggtc
     4981 gcatctacca gagttgcgcc cttccagaag agcacatgcg agccatcgaa agcaccctcc
     5041 gcaccgatgt cttcctttcg catccccggg cggcgataga acgtgaacgc tccgtaggcc
     5101 ccagttgcat cctcaaagcg gatggcccgg acactcagct tgttgtccgg ctgaacataa
     5161 tttgcagatg caaattcggc aaagccatat tcgtgcagca ccggggcatt ggcctgatcg
     5221 gcggcctcag gagattggct ctgctgaaca gggccagacg catgccatcc ggcgaaatcc
     5281 gaaggcaaaa aagcttgctg ctgctgcgaa gaagggggag cggccttttg cgcagaaagc
     5341 tgggccaggg agaacaaggc gagcgcgata ggaaacaagc ggcgcaacat aaatactgcc
     5401 tgacttgaga ctaccaccgc aggattttct gtgctggcgc ttatggtttg ggccgtgctg
     5461 cctttccttc aagaatgttt ccgccaaagc gaaatgccag acctgccgaa gcacgggtgg
     5521 tccagcggct gtccttcaat aaatcgttga acaggtggtt gtagacctgg tctgcctgaa
     5581 aacgcagggc gaggtgacgc gttacgttcc agtccagacc gccgccgaag ccgtaaaagc
     5641 tggtccagct tgtcttgtgc ccagatggcg caagctgctg cacgatgagg gccgcgatgg
     5701 gatcgcgcgg atgcggcgtt gcttcttcac ggatggcgcc gagatcgggc cggacgaaaa
     5761 gcgtgacagg aacaaagtgc ctgtacgaaa actgtgttcc cgcagcgaag gtctgggtcg
     5821 tagaatccgt ggggacggaa agctgatacc cgggcggaag cagtccggcg agcgcaaggt
     5881 ctttgagggc ggttgctacc tgcaattgca gagagtcggt cagcaggttt ggggtgaggg
     5941 agttgtgccc ggtcacgatc gagtaatcga agccaagtgt gaggatgcgg gtcgcattgt
     6001 atccggcctg aagatggtat ccatgctcgg ccagattcag gctgggcgag ttgaagaacg
     6061 taaatccaga gtagagatcg aagcgttcga cgtcggtttg ctgtgcggtt gcgtaaagcg
     6121 gaaaggaaaa cactgcggcg aggatgaaag caaacagtct ggcagtcatg cggtcctccg
     6181 tggggacaaa gaacagcgcc ttttcaaaga caatcgagat tactcacgcc ggagggaaat
     6241 tggcaagtaa aatcggttat caatcagtta tctttgaaca gctttgcaag atgaaagtac
     6301 cgtcgcgcct gcttcacatc acgaagaatc tgctctttca atgtctcagg cgacggaaag
     6361 cgcatctctg gacgaatacg gtgaagaaag gtgagttcga gtgacgttgt tccagtaagc
     6421 tcaatgggcc ggaagtcgag cagatgcgtc tcaacagtga aggaggcatc tccaaacgta
     6481 ggacggacgc cgatgttcgt gactgaatcg aagaccgggc catcgccaat cttcatccgc
     6541 gtgatgtata cgccatctgc cgggagcagc tcgtcatatt gcgcaagatt gatggtcgga
     6601 acggtgagct ttgagccgat gccgcgtccg cgcgccggag tggagcggat ggagaacggc
     6661 cgccccaata gagctcgcgc agcagtcatg tttccggcgg caatcagctc gcgtagcttg
     6721 ctgctggaaa ccggaaatcc gcgcacgatg cgcgtgtggt gggcagtcac ggcgaatccc
     6781 agaactctgc ccagcgccat cagctccgta atgcctgcct gcgcctgatg tccgaagcga
     6841 aaattatcgc cttcatggac ttccacggcg ttgaggccat cgcgcaggac attgcgggca
     6901 aagtcctcgg cggagaggtg cgagaattct tcggtaaagg gcagaacaac ggtggcatca
     6961 agaccggttt ccgcaagcag gcgcaggcgc tccgccatag gagtgatcag ccgcggcgca
     7021 atttcaggcc gaagcacgcg taccggatga ggatcaaaag tgacggcgat gcgttttgcg
     7081 ccgagttccc ctgcgcgttc acgggcgtgc tggatgatcc agcgatggcc gcaatgcacg
     7141 ccgtcaaagt tgccgatggc gatgaccgtg gggccatagt tggctgggac ctcagaaagc
     7201 gcgcgaaata cctgcatatc tatagctcaa acggaatgcg aagtccgtca tatgcgaggc
     7261 ggacatgcgg agggagttcg tcttcggttt ctgcatgaag cagatcatgc gacatatgtg
     7321 taaaccaaac acggcgcggt ttcagtactt ccacccagtg cagcgcctcg tcaatattgg
     7381 cgtggctggg gtgcggtttt ttgcgcagag cgtccatgat gaccacatca aggttttgca
     7441 gcaacggaag gctgccatcc gggatgcggt tcatatctgt cagatacgct gcgctgccga
     7501 agcgaaaccc ggctgcctcc tgttcgccat gaatcagcgg cacacgctga aagatggcat
     7561 tctgaatggt caccgactcc gttctttcca gccggttcat cttcacacgg gcgcgtgttg
     7621 gatatctgga ctcagcagaa aaggtgtagt cgaagacttt ttcgatgatg cgcgccgaga
     7681 aatcgtccgc atagagcgga atgtgaccgc tgggatgatg aaagctcagg gggcgcaggt
     7741 cgtcgaggcc gagaatgtgg tcggcgtgcg agtgggtgta aaaaacggca tccacatgcc
     7801 ggatgttttc acgcagcgcc tgcgtacgga agtcggggcc ggtgtcaatc aggacgcaat
     7861 gcccacccca ttgaacagca gcagagggcc gcgtgcgccg gtctcgtgag tcaggcgagg
     7921 tgcagacatg gcattcgcac ccgagcgtgg gcacgcccat ggatgtcccg ctgccgagga
     7981 tggtgagtgt ggcttgcatg tagcagtaaa caggtttgtc atcctgaggg gcgaagtgac
     8041 aggggactca gctgagattc ttcactacgc cttcggcttc gttcagggtg acggtaaatc
     8101 gaaaaatact acttctttgc cagtggaggt tgctgcgccg atttcgagat ggcgttggtg
     8161 atctcgacgt acatcatgcg caggtcaaag agggcgttct gcaacagacg ctgctctttt
     8221 tccgtgagat tgcctttcgt cttctgctcc agaacggcga gcatatcaat gctctgacgc
     8281 gcgccaacaa tgtcaatgcg tggctgctgg cctggttctg tgcctgcacc catggccacc
     8341 agcgccgaca gataaataga ctggatgaca tgctcgaaag cgacttcctg cggcgctccc
     8401 attccgggat tggcctggcg cagcatgtca tctatctcgc gtgtggactg ctgataggcc
     8461 tcgtgctgcg cggctgactc ctgcgcagtc ggtccggcgt gctcttcagt ggcaggctgc
     8521 ggtgtttcct ctgccctcgg ggcctcctgt ttcggtggag gcatcgtaat cacgcgaggg
     8581 ccattttccg ctggtttttc ctgctgctct gattgcgcag ggtcgtcgcg ccgctctcct
     8641 tccggagtga acttgcggcg gtcggtgacc gtaaatgctg gtggtttatt ttccatgatt
     8701 tccttcctga tcagggcaag gccaaaggtc cttccagggg cgttgcagtg cctccggcat
     8761 atgtaatggc tgccttgcgt tctaaaactt cgatgcggac aaatccaagc gcgagcgttt
     8821 gcgtaaagcc gggcagcgcg taatgggagg cgctggtgat ttcaccgaca ggtttatcgt
     8881 cgccttccac gtgaagctcc gcgcctgatt ggggtaaatc cccatgcaag tcaaactgtt
     8941 tcagcgtgcg gtggaccgta gcgcgggagc ggatgcgctc gacaatctcc tgcccgagat
     9001 agcatccttt actgaagttc agcgcgcgca tctggtttgt ttcctgcggg agggcacggt
     9061 cggtaatgtc cactccatat tccgggacac cttcaaagat gcgcagcgaa ttgagcgcgg
     9121 caaacccggc gggtttggcc ccggcttcgg tcaaaatgcg ccacatttcg cccgcgtgct
     9181 ctgaggcaag ccataattca aagcgcggaa ccagtacttc ataagcactg acgagggtga
     9241 ccggaatccc ctggatggcc gtttgggcaa aggtcatctt ttcgggaata tcaatcccca
     9301 gagattcaag aatggcagga gcttccggcc cggcgagacc tagagtggtg ttcgctgcgt
     9361 ctaactcctg taggtccaca tcatccatga tgatgaaatg atcaaggtgc gccaggagac
     9421 gcggcaactg caaccggtcc gtttcaaaca ggtaatggtc aggcgagcgg aagatatagc
     9481 catcgccctg aatgcgccct tgcgcgttca aaacaaaggt gtacaaaccg gagccttcgg
     9541 ccatatcacg ggtattgttc gtgaccattc cgttcagcca gcgcacgcgg tcttttccgg
     9601 tgatgcggat gcgcgtcagg taaccgagat cgtagaccgc ggcggagtgc atcagatgac
     9661 cgagttcgat ggtgcagtcc tcgaagacat ggggcgtaag ccggccacgg tgcatctgaa
     9721 ggtcagatgc aacatggaga gaggccaacg gagtgcgttc attctcaacc agtggtggcg
     9781 tttgacccat aacaaaatta tagtcgggta aggatgttca agatgaaaac agcaggcagg
     9841 ctggttctgg tggtgatggt tgccgcagga atgtgcctgg ggcagaccac ggacaacgct
     9901 gcggcgcaaa aagttgctcc tcacgaaacg cacatcacgc catcgcaggc aaaggaactc
     9961 ttccattccg ttggagtgat cctgaagttc gcttccgagg atacggacct gcccatccgg
    10021 cacgatgtga aacgcaaact aaccacgcgc gaggacgtcg agaagtacct catccggaag
    10081 atggacgatg acaaggatgc taagcgcatg gagcgctctg agatcgtcct gaagaagttc
    10141 ggcctgcttg accgtgattt ccagcttcgt ccattcctgc tggcccttct gaaagagcag
    10201 attgaggcct attacgacag caagaccaag acagtgaacc tgctcgactg ggccactcct
    10261 gagacgcaga agcctgtgct ggcccacgag ttgacgcacg cccttcagga tcagcacgtt
    10321 gatctggaca agtgggaaga cgtaaccagc gaagacttgt cccataatgc gaagcaggac
    10381 aatgagcata tccagactga cgaggtggat accgcacgtg aggccgtgct cgaagggcag
    10441 gccatggccg tttatgtgga ctactcactg gccccctcag gcaagagcct gctgaatgct
    10501 ccagacctgg atacggatga cctgggcggc gacacctccg attcgcctgt gctttcgcgc
    10561 gctccactgg tgctgaagga gtcgctgctg tttccttacc aggatggcct gcattttgaa
    10621 caggtcctgc tcaagaacga aggcaaagat ggagcctttg ccggtgtact cgaccgccct
    10681 ccggcgtcga gctatgaagt gatgaacccg cgtgcgtatc tccggaagca gaaggccccg
    10741 gtgttgagta tgccggacgt tcatcccctt ctcgatgcgg attacgaccc ctatgatgtc
    10801 ggcgtgatgg gccagttgga cgtgcgaatg ctgagcgagc tgtttggcgg agagcagctc
    10861 tcgaaagtgc tcactccagc gtgggacggc ggcatctact atgccgtgca gagcaaaaag
    10921 gcaaagaccg gcgcagagaa agattcaacc gcatccattg gactactcta tctttcgcgc
    10981 tggaagtcgc ccgaggccgc ccgtgccttc gccaaaatgt acgccgatga actgagcaaa
    11041 aagtattctg gcgtaaagcg cgatccggat gcagaaggag acccaaccga gcaggtctac
    11101 cagaccagtg aaggccccgc tctcattgtc accaaagaca atatggtttt tactagcgag
    11161 agcttcgatc tgccgctcgc ccgcaagctt gctttcctca tgatgggggc acaggaagga
    11221 ggcggaagcc ttgaagctgc aaaattcaag gccccgggtc atccttcgca tgaactgacc
    11281 accagcatgg tccgcttctt taccggatgc ggcatggtac gcgcggccgt gcgatagatt
    11341 taagcgaggt gcggaccgct gcatccttcg ctttgccgca tcaccggtat acttaccgcc
    11401 cgtcccaggc cttcgggcag ccttttccgt cttctacggc tgtcagcaat cctgctgcgg
    11461 atctaagctc gcggctgtag cagtagccag tcgaatcgcg cgtgaagtac ttttgcgaac
    11521 tgctgcggtc cgttgttccg gtgagtgatc cgttcgcacc gaattgcagg gtgtcggcgg
    11581 tattcacggc gttgctggtt tcagattgat acagcgggaa accattcagc aaaagtgttt
    11641 cgtgttcgtg aaatccctgc tggacagtgg tcacctgctg gctcgatcca tctgtatttg
    11701 tggtgtagtt atatgccaga gagagcgggt acgagagctt cttgtgtttc tccgtaacgt
    11761 accagccatc tctggtgatc gtgcgggaag atatctccgt gccctgcacg atggcctgtt
    11821 catactgcgt gttgctgatg gtgaattgct ggttgttagt gaaggtaatg ttctgctcga
    11881 cggtggtttc gatgcgtcca tgcgatgtat tcacccatcc ggaaatgcgg taatttcgca
    11941 ggttggacgt gtccactgtt ccaccgctgg agcttacgcc ctggtcctga atcacagggg
    12001 caggagcggc agccaaggtg ttctctataa ctgctccggt tacagacttg ccgccgtggt
    12061 cctcaaagac cagcaggttg gcggtcacgg caaagtagct gtctgcattg aatacgctca
    12121 gcgcaatggt gtgcggcttg ccattcgaga gcagtccggc aaacggtgtc aggtctacgc
    12181 ggtacggaac aaagttcaga gtctgtactc ccggaatggg cgcccataga taagggtcaa
    12241 tgccgccggt gaaaatccat ggatagaccg gagcaacgcc ggctggctgt ccatcaatcg
    12301 tgatttccgt ctcacgaaat gctgtagagc cgcagctttg caatgtcgcc gtgtcagcat
    12361 tgggaacgca ggtgtaccaa aactcgtctg tagactgccc ctgcgccaga acgtcgagat
    12421 atgcgcgctc gacattcgta ggcagcgtga cagtttgcga tagctggttg gaaggcgtat
    12481 tcagatatgt ggagtccgtt gcgcctgtgg gaagagccaa gacctggtca gccgtgtgcg
    12541 gcgccggaga aaagaacgtg gccggataga aaagcaactg cgccgagccg tgaatgactc
    12601 cggtataggt ggagtcaacg acattatcca ggtggatgtc tccactctga ggttgtgtaa
    12661 acagtgcaga gtagtcagtc aggtcgcgct caatgtgcca ggtgggagcg actgttttgc
    12721 gcggttctgg tgtggtcccg aagtaaatgt tcacgccgtt cagaaacatc ttcgcggtgc
    12781 ggtcaaactg gcgtcctgcg ttctcgttga aatcggcgac aagcaccact ttggcccacg
    12841 ggccggggca attcgcaggc ggtatgtaag taaagttcct gggactgaaa tcgtcaaagg
    12901 tgaaatcgga aaacagtgtt acggtgcacg gtgtcgtatt cgggtggcgg actggcgggt
    12961 cggcggtcgc tgtattggat gagccgacta caggcgtctg cgccagaccg aaagaaagag
    13021 ctgcggcgcc aaaaagcgcg gcagcaagaa tttgcctcac tgggaaaaac ataggtctgc
    13081 tcctttaaga ggggatgagc aatgacagca gtgttgggaa ggataccatg tgaaccgctg
    13141 ccctgccact atctgcaacg caccctgccc ggggcaacga tttagcggca tttatactga
    13201 gacttcatca ctctcaccca ttcggagcag gatttgcagc aagcagaaat cgggatcatc
    13261 gggggcagcg gactgtatgc catgcccggc ctcaccaaca cgcgtgaaga gcgcgtagag
    13321 acaccgtttg gcgagccaag cgatgcattt gttcttggcg aactggaagg ccgcagggtc
    13381 gcctttctgg cgcggcatgg acgaggccac aaactgcttc ctacggagct gaactttcgc
    13441 gccaatattt atgcgctgaa aaagctcggt gtggagcgca tcgtttctgt ttccgccgtc
    13501 ggctccctca aggaagagca caagcctacg gacttcgtcg ttccagacca gtttattgac
    13561 cgcacgtttc atcgtgaatc caccttcttc ggcgatggac tcgtggcgca cgttgccttc
    13621 ggcgatcccg tctgtgccac cgtagccaaa gccattacag acggatgctc tgcttccggc
    13681 gtagtaggca agcagggggg cacctatgtc tgtatggaag ggccgcagtt ttcgacccgg
    13741 gccgaatcga acctttaccg aagctgggga gccgatgtca ttggcatgac caacctgcag
    13801 gaggcgaagc tggcgcgaga ggccgaaatt tgctacgcca caatggccat ggtgaccgac
    13861 tatgactgct ggcatgaggg ccacgacgat gtaaccgtcg agcagattat cgccgtgctg
    13921 aaccagaatg cggcgaacgc cgcaaaggtg gtgcgagccg cagtggcagc catgccaaaa
    13981 gaacgcaaat gcgcctgtgc aacagcgctg caatatgcga tcatcaccaa cccggcggca
    14041 gttccgcctg aaacacgcaa acggctggat ttactggtcg gaaaatatct caaaaaggac
    14101 taagcagaat gtcaattctc gtagtgggca gtgtcgcatt tgacagcatt gagacccctt
    14161 caggaagggc agagcgctgc ctgggtggag cggcaacata tttctctctt gccgcgagct
    14221 atttcaccga cgttcgcgtg attgccgtag tcggcgagga cttcaccgcg aaagaagaag
    14281 atgtactgaa gcgacgcgac attgatacgc agggactgga gcacgttgct ggaaagagtt
    14341 tcttctggcg cggctcctac atgaaggacc tgaacgaggc gcagacccag gagacgcagc
    14401 tcaacgtctt tgagagtttt tcgccgaaaa ttcctgctgc atacactgat tcagaatttc
    14461 tctttctcgc caacattgat ccggtcctgc aggacaccgt ccgcaggcag atgccgaaag
    14521 tccgtatggt gtgcggtgac accatgaact actggatcac cggtcatcgc gaaaaccttg
    14581 aaagagtgct gaaggggctg gatggtcttt tgattaacga cggcgaagcc aagctgctgg
    14641 cgaacgacaa caatctggtt tgcgcggccc agaagattct ggcgatgggg ccgaagagcc
    14701 ttgtagtcaa acatggtgaa tatggcgcaa cggccttctt cagcaaccgc ctttttcccg
    14761 gagagacgaa gcatgtctcg ctgcctttcc gcgctcccgc actgccgttg agcgaggtcg
    14821 tagacccgac cggagcaggc gactccttcg ccggaggctt cttcggccat ctggcttcgc
    14881 agaaagaagt gaccccggca tccttcaagc gcgccatgtt ctacggaggc gtgatgggat
    14941 cgttcgccgt cgagcgcttc ggcaccgaac ggctccagtc cctcacacgc gaagagattg
    15001 accagcgttt tgcgctgttc cgtgagttgt cacaccttga ctgatcctgc aactcaaaaa
    15061 aaaaggatgt cccatccttc gcatagcaaa gagtgggaga ccgaatcgct ccgcagtgag
    15121 acatggctcg tggcctgcgt ggccctggct gtgtctctca tggcgctggc atactgctct
    15181 ccgcgcggaa tgctcctgct ctatggtgat gccgtggcgc atctgcacat cgcccgccgt
    15241 gtcttcgatt cgctcgatcc cggatacaag cagctaggct cggtgtggct tccgctgtcg
    15301 cacattctgc tcattccttt tgtgcagaag atgtcctggt ggcaaacggg tctggccggg
    15361 gcatggcctt ccatggcgtg ttacgtgctc ggatgcgcag gcatctaccg tctggcgcgc
    15421 atctggttgc ggccagcagc tgcatggatt gctgtcgctt tctttgcgct gaacccaggc
    15481 ttgctttata tgcagaccac cgccatgacc gagccgctct ttctggccga gatggtctgg
    15541 agcgtactcc tgctggccgg gtacgcccgc gctctcaagt cttcagatgc ggtgcgtgca
    15601 tcgcgtttgc tgattggcct gggattcgtg cttgcaggcg ctgtctacac gcgctacgac
    15661 ggatggatat tcgcctgcat tgcctggtgg attgcgttat ggaatctccg ccgccactgg
    15721 agcagtcgcg caggtggagc attcatcctc tttaccgcta ttcttgtcac tgctccgctg
    15781 ttatggatgg cctacaacgc gcgccagttt ggcgaccccc tcgacttcat gcgggggcct
    15841 tattccgcaa aggcgattga agaacgcacc gctgctccgg gggccccgca ttatcccggc
    15901 tggcacagcg tgagagtcgc ggcactttat tttctgaagg ctgcggagct cggtgccgct
    15961 cccgttcgtt tggccaacct gatgctctgg ctcagcgttg ccgggaccat cgctgccttt
    16021 tggaaatggc gtggacagag catcacgtcc gcgctgctgc tttgggtgcc gctcccgttc
    16081 tacgcatact ccattgctta tggatcggtg cctattttta tcccactctg gtggccgcac
    16141 tcctggtata acacgcgcta tggcatggag atgctgccgg cctttgcgct ttttcctgcg
    16201 ttcctcgtcg cctgggccgc aagacaatgg ccgcgcgcgc gaagatgggt aatagcggtg
    16261 gcactggctc tcatcgttgc agacagcatt acgctgatgc gtaaccgtcc gattgtcctg
    16321 gccgaagcca tcgccaattc gcgcacgcgt attccttatg aggaatcact ggctgaagcg
    16381 ttgaagtccc tgccacagca gggcatgatt ctgatgtata cctcggagca cattggggcc
    16441 ttacagcgtg cagggatccc gctgaagcgc accatcaatg agacggacta ctaccaatgg
    16501 cccgcagcct tgcagcatcc ggcaagggcc gcaagtattg ttgtcgccac agatcatgat
    16561 gccgtggcca aagccatcgc cgcgcaccct gaagggctga tgctggttga cgtagtgtgt
    16621 tccaccgggc agccttgcgc gcgcatttac ctttcaaagt ggtagcatca cgctcaaaca
    16681 gccatgatga tcgaagcccc cggacacatg ctgagtttcg acgccttgca ggaggccatc
    16741 ctcacaggag gcgctgcggg gcgcctgctg ctggccgcat ttctcggcgg cgtaattggt
    16801 attgaccgcg aatatcatca caagccgtcc ggcgtgcgga ccaatctgct catctgcttc
    16861 ggcgcggcgc tgtttacgtt tctttcgtcc gttgtagctg gcgaaggcag caccaataaa
    16921 ggacagattg cttccaacat cgtccaggga atcggctttt taggcgctgg cctcatcctg
    16981 cacaaccgcg accgcgtcag cgggctcacc agcgcggcga ccgtctgggc agtggcttcg
    17041 attggcatgg cctgcggcgc tggattgtac atcccggcag tgttctcggc cgtgctggtt
    17101 ctggtggcgt tagaaggcgt ggggttgctt gagcgcaagg ccaacctcaa gctctattct
    17161 gttatctatg aggcgcgcgg acgcgacccg gtaaaaatgt ccatgactat ccttcatgcg
    17221 atggacagaa tgaagcggcg tctcgggcat ctggagcagg agaatatcgg cgacctcaac
    17281 cgcatctgct ttaccgtcta cgccacgcgc aaagggcacg accagttgca ggcgaccctg
    17341 aatgcctcac cagcgattga cgagatacat atatttccga atgtggagga tgattgatcc
    17401 cctttgtaaa agcacatgcc tgtggcaatg attttctggt ggtcgaggca aagcatctag
    17461 gcgcggaaaa gcagcgtgaa acggccatcc gtctctgcgc ccgcaataca ggagtcggcg
    17521 cagacggcgt cgagtatctg gaatggacag gcgagcgctc cggccgcatc cggctcttca
    17581 acgccgatgg ctcgattgcg gaaatctccg gcaacggtac ccgctgcgtt gccgcatgga
    17641 tggcccacga aaagggcctc cagcgaggag acgttcttac gctcgaaacc gatgccggcc
    17701 cacgcgagtg ccgtatcgtc ctcaatgaag atggccgcta ccagattgcc tcacgcatgg
    17761 gcgtgccgga aatcagcagg cgtggcgtgg atctgaatgg cacgcagatt gaaggggtgg
    17821 ccgtctccac aggcaatccg cactttgtca tctttgtgca tgacgggaca tttacggcgc
    17881 tgaatcgcag ttggcaggag ataggccacg ccatctgctt tcatccggat tttccgcagc
    17941 agaccaacgt ggaatttgta cacatcctca atacaaacga aattgaaatc cgcatcttcg
    18001 agcgcggcgt cgggccaacg gcttcttccg gcaccggaac atgcgcttcg gccgtggctg
    18061 cgatttctca gcgcggcgca gcgaaacatc tgctggtacg cgcgcccggc ggcgagcaga
    18121 gggtcgagtg ggatggcgag ggaagcgaga ttctgctgac cgggccggcc cagttgatcg
    18181 cacaaggcga ggcattctag atgctgaagc cgcctgccgt agcgccggat gcacgtgtcg
    18241 ccgtgattgc cccagccagt tcttccaaac cagagcggct ggcgcgtggc attgaggcgc
    18301 tgcgcgcgcg cggatacgac gccgaatttg gccagttcgc gcagggacgg cttgccccct
    18361 atttttccgg aacggcagag gaaaggctgg cagatttcca cgccgctttt tctgatcctg
    18421 aaatacaggc catcttttgt acccgtggcg gttacggatc gaactacctg cttgaggaca
    18481 ttgatctcga cctcattcgc gaaaacccga agccgctctt cggctatagc gatatgactg
    18541 ccgtgcagac gtggcttctt gaccagaccg gactggtggc cttccacggc ccgatggtgg
    18601 ccgcggactt cgccgtgagc ggtggcgtac acggtcccag cctcgatgca gcgctcgatg
    18661 gcgaccttat ttctgtcagc gcggccgagg ggcttcgcat cctcaggcca gggcgcgcgc
    18721 gcggggtgat gtacggcgga tgcctcagca ttctcacttc aaccctgggc acccgctttg
    18781 cgccgcagac cgaaggcaaa ctgctcttcc tggaagatgt gggcgcaaag ccctaccaga
    18841 ttgaccggat gctgcgccag atggtgctcg cgggcaagtt cgacggagtg accggattca
    18901 ttttcggaga gatgctcgaa tgcgcatcgc ccggggcccc tccggattta ctggaacagg
    18961 tcatcctgcg cgtgctcgac tggtttgacg ggccggttgc cattggactg cgctccggcc
    19021 acgtttcgca cagcaatatt acgctgccgt ttgggattga agccgaatta attttggaag
    19081 atgctccgga gctgcgctat ctggagccgg cagtagagaa tgaatagcaa gagcagagag
    19141 cgcgccccgc tcaggtgaca atagttagaa gatgcaaagc aaacacattc atctcatcgg
    19201 aatctgcggc acagccatgg cttctcttgc cgggctgctg caactcaaag gacatcgcgt
    19261 caccggctcc gaccaggcgg cctatccgcc gatgtccgac ttgctgtgca gcttgcggat
    19321 tccggtcgcg gagccgttct cggaagagaa cctgaagccg catcccgacc ttgtcatcat
    19381 cggaaacgca atttcccgcg gcaatgttga gctggaatat gtgctcgacc agcgcattcc
    19441 attcacctcg cttgcagcgt tgatgcatca tgagtttctg cgagggcgcg agtcgctcgt
    19501 cgtagccggg acgcatggca agaccacaac cacctccatg ttggcgtgga tgtatgaagt
    19561 ggcaaagctg gccccatcat tcctgattgg cggcgtggcg gaaaacttcg ggacgagctt
    19621 tcagctccac gacagcaggc cgttcatcat ggaaggcgat gagtacgaca cggccttctt
    19681 cgataaaggc ccgaaattca tgcactactt tcccgacgcg ctcattctca cacacgtcga
    19741 gttcgatcat gccgatatct accgcgacct tgaccaggta aagaccgcct tcaagcgtct
    19801 ggtgaatctc gttccccggc gcggacgcat cgtcgccttc gatggcgcgg ccaatgtatc
    19861 tgaatgtgtt gcgcgcgcct tttgcaaagt agaacgctat ggtttcgcac cagactccag
    19921 ctggcgcatc acagacctgc attttgcggg cggccagacc cgttggaagg tgctgcgtga
    19981 tggaaaacag tgggcagatc tggcgttgaa catggccggt gagcacaacg ccctgaacgc
    20041 aatggccgcg gccgcactcg ccgcaggcca aggggtgcca atgtctgcca tccaggaagc
    20101 cctggcaacg tttaaaagcg tgaagcgcag gctggaagtg cgtgcggtgg tagatggagt
    20161 gaccatcatt gacgattttg cccaccatcc tactgcaatc cgtgagacac ttcgtgcctt
    20221 gcgtaccgcg tatccggatg cgcgcctctg ggccgttctc gaaccgcgct caaacactct
    20281 acgccgcaat gtttttgagg ctgagttggt agacagtctt tcgcgggctg atcacattgt
    20341 gttatcaggg gtctttaaac aggagagcat tcctgaaaat gaacgcctgc atccggaaac
    20401 gatcgtcgcc gcgctgaagc agtccggcaa aagcgccgcg ctctttgcca atgctgatgc
    20461 catcgt
//
LOCUS       DASRBV010000096         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_211162, whole genome shotgun sequence.
ACCESSION   DASRBV010000096 DASRBV010000000
VERSION     DASRBV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211162"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFA02_03845"
     CDS             <1..1098
                     /locus_tag="VFA02_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU09010.1"
                     /translation="SADITDLSSETCLPGLMDTHTHVLLQGDATAAEYAEQLLKQSLA
                     YRGIMGAVNAARALNYGFTTIRDLETEGALYADVDIKHLIEQGVIPGPRMQVATRALD
                     VTGAYPLLGYAYGVDVPHGVQTCDGPEECRKAVREQISYGADWIKVYADRGYFMRPDG
                     VLDDVPTFTLEELNAIVDEAHREDHPVAAHAMGLHGVHNAVTAGVDTLEHGEYIAPED
                     LKTMAEKGIWYVPTTYALEAVSEGRASDGNPVYKQMIKTHSETMRRAMEAHIRIAFGT
                     DAGAFPWTTNPAKEFAVLVRDGMTPAQAIQSATIQAAKLMRMDDQLGMVGMGKLADIV
                     AVPGNPLGQITLMEQVNFVMKDGVVYRSPKP"
     gene            complement(1031..1849)
                     /locus_tag="VFA02_03850"
     CDS             complement(1031..1849)
                     /locus_tag="VFA02_03850"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZU09011.1"
                     /translation="MGPLSGKTIIVTRARHQASRLTRELESLGARVVEIPAIEIVPPE
                     SYAPLDAALRNLHQYRWLIVTSANTVRVLGERIASFGLGPEAFAHINCVAIGSATADA
                     LRNFGLSVALVPKEYVAESLIEALHGQVAQSCILLARAAAARDIVPDELRKAGAIVDV
                     VDAYRTVIPASSVELIRKVNGIATDAITFTSSSTVTNFFQLLKSAGTDAMPDNIKAIS
                     IGPITSATLREHGWEPAAEANPHDVNGLVQAVLTALGCDTPPRPSSQNSPAPST"
     gene            2315..>2704
                     /locus_tag="VFA02_03855"
     CDS             2315..>2704
                     /locus_tag="VFA02_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HZU09012.1"
                     /translation="MAQQLAMMRSPAAYAGVTAYARAHTGEASAAAYIALGHAYLLDH
                     KYPEAVSAFRQANIQGNALDDYADYLEAQADLQSNNLPQAETILSAFVQKHPDSIFVS
                     SIPVLQANLFLQEGDPQAALRILDAHKG"
BASE COUNT          600 a          685 c          860 g          559 t
ORIGIN      
        1 agcgctgata tcaccgacct ttcaagcgag acatgtctcc ccggattgat ggatacacat
       61 acgcatgtac tactgcaagg ggatgcgacg gcagcggaat atgcggagca gcttctgaaa
      121 cagtctctgg cgtatcgcgg cattatgggc gcagtaaatg cagccagggc actgaattac
      181 gggtttacca caatccgcga tcttgagacc gaaggggcgc tctacgccga cgtggacatc
      241 aaacacctca tcgagcaggg tgtgattccc ggaccgcgga tgcaggtggc cacacgcgcg
      301 ctggatgtga cgggggcgta tccgctgctg ggatatgcgt atggagtgga tgtcccgcat
      361 ggcgtgcaaa cctgcgatgg gccggaggaa tgccgcaagg ccgtgcgcga gcagatttct
      421 tatggcgcgg actggatcaa ggtctatgcg gaccgcggat attttatgcg tcctgatggc
      481 gtgctggatg atgttcccac cttcacgctg gaagagctga acgcaattgt ggatgaagcg
      541 catcgggagg accatccggt ggcggcgcac gcgatggggc tgcacggcgt gcataatgct
      601 gtgacggcgg gggtggacac gctggagcat ggggagtata tcgctccgga agacttgaag
      661 acgatggcgg agaagggtat ctggtatgtg cctacgacgt atgctctgga agcggtatcc
      721 gaggggaggg cgagtgatgg aaaccctgtg tataagcaga tgatcaagac ccattctgag
      781 acgatgcggc gggcgatgga ggcacacatc aggattgcat tcggcacgga tgcaggggcg
      841 tttccctgga cgacaaatcc ggcgaaggaa tttgcggtgc tggtgcggga cgggatgact
      901 ccggcgcagg cgatccaaag cgcaacgatc caagcggcaa agctaatgcg gatggatgat
      961 cagctcggta tggtaggaat gggaaaacta gccgatattg ttgctgttcc gggcaatccg
     1021 ctggggcaga ttacgttgat ggagcaggtg aattttgtga tgaaggacgg ggtggtgtat
     1081 cgcagcccaa agccgtgagc acagcctgga cgaggccgtt gacgtcatgc gggttggctt
     1141 ccgcggccgg ctcccatccg tgttcgcgca aggttgcgga agtgatgggg ccgatggaga
     1201 tggctttgat gttgtccggc attgcgtctg ttcctgcgct tttcaaaagc tgaaagaaat
     1261 ttgtaactgt ggatgagctt gtaaaggtaa tggcgtccgt ggcaatgcca ttcactttcc
     1321 gtataagttc taccgaagac gcaggtatta ccgttctgta ggcgtccaca acatccacga
     1381 ttgcgcctgc ttttctcagt tcgtcgggga cgatgtcgcg ggcggccgcg gcgcgggcga
     1441 gcaggatgca cgattgggct acctgaccgt gcagtgcttc tatgagcgat tcagccacat
     1501 actctttcgg aacaagagca acggaaaggc cgaaattgcg cagcgcgtct gctgtagcgg
     1561 agccaatggc gacacagttg atatgggcga atgcttctgg gcccagacca aaagaggcga
     1621 ttctctcacc gaggacgcgg acggtgttgg cgctggtgac aatcagccac cgatactgat
     1681 gcagattgcg cagcgcggca tcgaggggcg cgtaagattc cggggggacg atctcaatgg
     1741 caggaatttc gacgacgcgc gcgccgaggg attcgagttc gcgggtgagc cggctggcct
     1801 gatggcgggc acgggtgacg atgatggtct ttccggacag aggccccata agaacctgta
     1861 ttcgattcaa cagagtaaca gtttttgagt cagctgaagc cggccatttt cgcgtttcct
     1921 ccgttcctga tcaggaacgt tttttcctct cgcccgcatt gcggagcaac atttgtcctc
     1981 atttcgtctt acattgaata caggcaggag tgtgcagaga gcagtgaggg gagtatcgtc
     2041 agcgtggttg cgggtcatcg tcatcgttac cgtcagcatg gtcacagcga gatgctggcc
     2101 ccagagtagt tcatctaccc ataaaaaaca taagacaaca gccacggcca gcagttccaa
     2161 aacacaccac acaagccata ccgcgcataa aaccacggcc gcaacgcgca gcaggcatgc
     2221 ggtacacaga cgtcggcgtc ctttgtcagc gcgggcacga gcaaagagcc acaagctgca
     2281 gcaggcgttc gttgcttcat ctcagttacg gccgatggca cagcaattgg ctatgatgcg
     2341 cagtccggcg gcatatgccg gcgtaactgc gtatgcgcgg gcgcatacgg gcgaggcgtc
     2401 ggctgcggct tacattgcat tgggccatgc ctatctgctt gaccacaaat atcccgaggc
     2461 ggtgagcgcc ttccgtcagg caaatattca gggcaatgct ctggatgatt atgcagacta
     2521 tctggaggcg caggccgatt tgcagtcaaa caatctgccc caagccgaga ccatcctgag
     2581 cgcctttgtg cagaagcacc cggacagcat tttcgtttcc agtattccgg tgcttcaggc
     2641 aaacctcttt ctgcaggaag gcgacccgca ggccgcattg cgaattctcg atgcacataa
     2701 aggc
//
LOCUS       DASRBV010000097        24888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_211313, whole genome shotgun sequence.
ACCESSION   DASRBV010000097 DASRBV010000000
VERSION     DASRBV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 24888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24888
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211313"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFA02_03860"
     CDS             <1..1410
                     /locus_tag="VFA02_03860"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU09013.1"
                     /translation="RNPELLASVAPGTTLTGLRQQQSELMVQYAQLKAKYGPEWPKLK
                     EAEKQLATVQTNIDAEINNLTNKFQEEYNAAVNTENLLRSRLDQIKQEAYRTNASEAE
                     FDILKHGADSASDLYDALQTKLKEAGITAGLNSNNIDVIDRATEPARPVSPKKRIDLA
                     VGFMGGLLIGVMIALVLESLDDTLRTSEDVEAVSGLPSLAVIPHFTSIRKIASSSADG
                     AAAMDKTGVSAEIVSYTEPHSIGAEGYRTLRSSILLSSVDREPRLILLTSSYAAEGKS
                     TCAANLAISFAHRSARVLVVDTDLRKGTLHLKFKTSNRAGLSTLLARESGEEAYERPL
                     PDLPNLVVLTRGPIAPNPGEMLASRSMEDLLLQWRLEYDHVILDTSPVLPVADTLSLA
                     PHVDVAFVIVRAGITRKKALLKVRDQLRRVNARLLGTVVNDVDLRLENYYTYSKRYDY
                     GYKGYGAGYGVKDAQDGEK"
     gene            1397..2341
                     /locus_tag="VFA02_03865"
     CDS             1397..2341
                     /locus_tag="VFA02_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU09014.1"
                     /translation="MAKSKAIVLSLFVFVSIWQCRLLFGQTASQEAQIPQSAQSPSIA
                     IGPGDLLSVVVFDTPELSTSVRVSQDGEVNLPVLGRIHLAGVNTIDAARKIEQELRTR
                     RLLLEPHVTVFIAEYASQGATVMGEVRTPGVYPTLGTRRLLDMISVAGGLQPTAGKTA
                     SIIHRDDPQHPVNITLQSHPGALSAQENPVILPGDTIVVAKAGIVYVIGDVLKPGGFL
                     IDNNTPISVMQSLSLAGGWDKTAALSKVKLIRKTPEGREEVDLDLKHVTNGSQADLKV
                     TDGDIIFVPSSVGKTMAYRGVEAAISTATGIATYGHIY"
     gene            2402..3862
                     /locus_tag="VFA02_03870"
     CDS             2402..3862
                     /locus_tag="VFA02_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09015.1"
                     /translation="MDLTKKIVLVLSAVTAIATVSQIALGADFVILMMALCAIASGLA
                     GFYLLGSYNLVGWWFLLYVIGHVGVALVAKTLYRQPLESYLYAPFHSYLVEAVTCFIA
                     LCAIILVIWTNTGKPLFEKITDPGLLLFLSWSCFFIGSAVMLLFRLIAGGDKSGGASY
                     GGVAFLESLAYMGIIARTAYLAQASHGKKFLDSKLLFMLVALTGLGILAGGKSSTAVP
                     WLCFLLTVVFFAGKLPWRYVGFMLAGGLLFVLFLSPFIHILRGLQLRDASLGEEISLM
                     EHYLPLILNPDEFSFFAQRASTDTNYFHYFGSPKEGILLPRFTSVQQIDPVIVARDAG
                     DRMDTKLALDAVKANLPKFLVPDKPTETNAYTILVHEGYVPVYSTGHYPTLPYSGEIY
                     LIFGWPGVIAGSFALFFLYFLELKKIGWTLYCNIFAVFLLGSSLYHSNEMSLQDFIGQ
                     IIRDFPIFVLLIIFLVRAYYVFSRRILTKQESYAET"
     gene            3917..5116
                     /locus_tag="VFA02_03875"
     CDS             3917..5116
                     /locus_tag="VFA02_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09016.1"
                     /translation="MKTSRYAVYLLFMAILLMSAPSFAQLDSTSVSTPISSPVGGPLS
                     ESNSSFAATTSTEIETGASNLLNPDVRDAMTTDSVGADVSVTAMNPSLEKSVMVPPVL
                     SLPDTYDEQDALTLTGARRYSVPPLFGVLPPTRPANTPEALAFSHVTLPVSRFNSTAF
                     RVSVNSINANSFDPGVKAGQTFTGNHVQTPAWGQSALSENGMPANLPSGVDVFSAQQV
                     PSYPQSVSAQKRVQRPQNNPLSLGRQQQLSEQSGFSPRYVVRNLSMAAVGTSNQLTPG
                     SMDWSAWNTWPQDGFLNGTSLFRLNTLGEGNVFSPDIFNPRLKGNVRDRGGNNQMRIA
                     REQANRILRFSETKSVDSQTSNRLNQTYTLFPHTRRSQTQFKRYRYHNPLLNNDVSSQ
                     YTPDSQY"
     gene            5318..6733
                     /locus_tag="VFA02_03880"
     CDS             5318..6733
                     /locus_tag="VFA02_03880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZU09017.1"
                     /translation="MKEQLTIGIVGSGYVGLVTAVCFAELGHRVICVDSDEKKIALLR
                     QGKPPIHEEYLPQLLERHCNGAIEFTTDLKSATQRSQALFIAVGTPQSQAGNADMSYV
                     DAVASEIARAITSYKLVVEKSTVPVYTNEWISRIMMRNGVPRDLFDVASNPEFLREGT
                     AVVDFLHPDRIVIGVDSGRAADVLRAVYEPLTSGNYYKQAGAVPGPRMASGLPPLLET
                     STKAAEIIKHASNAFLAMKISFINVVSNICEAVGADIEQVSKGIGSDGRIGHKFLKAG
                     IGYGGSCFPKDVAAFRYVAEQMGIEFGLLSEVEKVNTEQKQRFFQKVRSALWTFRGKR
                     LGVLGLAFKGGTDDIRESPAIDLVRMFLQEGCIISAYDPAAMEKAKQVLPASESLSFA
                     KDAYEAAADADALLVLTDWAEFAELDLDRLSRILRYPVVIDGRNLFDPALMRQYGFTY
                     LGIGRAAGAHEKDPAFAYKLP"
     gene            6862..7854
                     /locus_tag="VFA02_03885"
     CDS             6862..7854
                     /locus_tag="VFA02_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZU09018.1"
                     /translation="MKLTNEKVVVCGAGGFIGGHLVKALLNQGVNVVRAVDVKPLDEW
                     YQKSKEVENLVLDLRDKDNCYKAAKGMQVVFQLAADMGGMGFIENNKALCMLSVLTNT
                     HMLMAAKDSGVERFFYSSSACVYNGDKQKSPNVVALKEEDAYPALPEDGYGWEKLFSE
                     RMCRHFREDFGLETRVARYHNVYGPFGTYEGGREKAPAAICRKVIQAKISGKHEIEIW
                     GDGHQTRSFMYVDDCTKGTRAILESDDILEPINLGSSELVTINQLVDIVEEIAGVKLK
                     RNYKLDAPKGVNGRNSDNTKILKYLGWEPSIRLRDGMQRTYEWIESQMLATVAR"
     gene            7876..8994
                     /locus_tag="VFA02_03890"
     CDS             7876..8994
                     /locus_tag="VFA02_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZU09019.1"
                     /translation="MADTQSFESAGIGGPVNRVLIYRLGSLGDTVVVLPCLHLIARVF
                     PNARRIMLTNSPVHAKAPATASVIGSSGLVHDYITYQTGSRSLAGFARIWWKIREFSP
                     DILIYLAAPRGDKAIQRDLSFFRLCGIRTMIGAPVGELAEPLYDAQTGLWEHEAFRLA
                     RTLAPLGNVDLENSESWDLRLTPAEINTASRVLAGLNGKQFLVAAIGTKMQAKDWGTE
                     KWQALMQRLGEVFPDHAMVFLGARDDFATCEKARSKWQGEALNLCGMLTPRESAAVIQ
                     RAELLMGPDSGPMHVAAALGVPCAAVFAARARPGHWFPFGKGHQVIYHKTDCFNCNLE
                     TCIEQRKKCIESVQVDEMLNAAVKAWEYGRRKREMQLA"
     gene            complement(9019..9609)
                     /locus_tag="VFA02_03895"
     CDS             complement(9019..9609)
                     /locus_tag="VFA02_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZU09020.1"
                     /translation="MIKMMQNVAEQDKFANVRYVFLDRDGVINRKPPEGKYIAKWDDF
                     HILPGAEKAIAMLNHSGRKAIIISNQRGIALGLYSKDDVLHLHEKLNQHLTCFNAHLD
                     AIYFCPHDRNECRCRKPGTALFEQAFLDFPSASSHNSVMIGDSLSDIEAGITLGMPTI
                     FIKGDPKHQKPGAEKAASLATAVADSLLTAVEQFLL"
     gene            9672..10280
                     /locus_tag="VFA02_03900"
     CDS             9672..10280
                     /locus_tag="VFA02_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HZU09021.1"
                     /translation="MTIDNASVFKGAIREHLSVVQQVEALIPALERIAREMLRCVSGG
                     NTVLWFGNGGSAADAQHLAAEFVGRFRRERCGLPSIALTTDTSVLTSIANDYGYEDVF
                     RRQVEALCSTGDVAVGISTSGNSKNVCRALEEARKRGAFTVAMTGEGGGLLASIADAI
                     LCMPSRDTARIQEGHILCGHMLCDWVEIAICQQAAHQKGQQS"
     gene            10277..11746
                     /gene="hldE"
                     /locus_tag="VFA02_03905"
     CDS             10277..11746
                     /gene="hldE"
                     /locus_tag="VFA02_03905"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HZU09022.1"
                     /translation="MITDLHRVVQFIEGDWKNKRVLVVGDVMLDRYVWGDVERISPEA
                     PVPIVRAGHKSSRAGGAANVAANITGLGAGSTLIGVIGADEDGECLKSCLDSMGVIHK
                     LVVSQKRPTTAKLRVFGGNQQMLRLDVEEVTECDGEIEEQILQHVKQVLPAMDALVLS
                     DYAKGVLSEKVCQSLIASAYRAGIPVLVDPKQRSFERYQGATTICPNLQELSTATGVS
                     CKNIEQILSAGQELVAALKLQYLTVTLSEKGIAILRKDSKDSFPAMARQVYDVSGAGD
                     TVIATLALSLASGLEIETAAQLANVAAGIVVSKVGTVPVERDELLASLMPEIELHAEE
                     KVLSLDRLKTRVSAWRSQGEKIVFTNGCFDLLHIGHIRLLQDARREGDRLIVAINSDK
                     SVAELKGPTRPIVGEQERAYVLAALSVIDAVVIFGEPTPLKLIEALRPDVIVKGGDYT
                     EETIVGAREVRSWGGRVKIVPTVPGFSTTKLIEKATIEA"
     gene            11803..11894
                     /locus_tag="VFA02_03910"
     tRNA            11803..11894
                     /locus_tag="VFA02_03910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11839..11841,aa:Ser,seq:cga)
     gene            12036..12404
                     /locus_tag="VFA02_03915"
     CDS             12036..12404
                     /locus_tag="VFA02_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09023.1"
                     /translation="MDTIDTTSSAIVLLMRTPSINLKFKTKVNEKWTFVPVARAGGKY
                     VPDRVVIHGKTIKAPEGSYYLEWWEVLKRRQRAVGANWRHAMDAVRIQTHVLALRAEG
                     AEDGTSGPEKSLPRDNGLTF"
     gene            complement(12432..12821)
                     /locus_tag="VFA02_03920"
     CDS             complement(12432..12821)
                     /locus_tag="VFA02_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09024.1"
                     /translation="MAEDQKLESLLSNLQEQIEATQAKLASLQKAYDALAPDGSLRTK
                     YRGIRTWVAIREYLREVGSASLHDIVDALLAGGADLGKYPLRTVKISVSSPHMKKLFK
                     AETVGNDEVVTLLERKDGLRMKARLTK"
     gene            13314..13562
                     /locus_tag="VFA02_03925"
     CDS             13314..13562
                     /locus_tag="VFA02_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09025.1"
                     /translation="MAKLFVSSDKSSLQRSVSDDAQGTLTSQSFLFVVSFLAFLSLKF
                     AVDYRAQQYKVIQRAFLIPGRLSLQRPFFFRPPPAFIH"
     gene            13678..14913
                     /locus_tag="VFA02_03930"
     CDS             13678..14913
                     /locus_tag="VFA02_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09026.1"
                     /translation="MVKLISYGRQFSALAISFCLAATLPGCRRTKDVKASTEPTAPVV
                     AVKQGDLATHLVVAGQFQPYQEVDLHAKVSGYIREIRVDIGDRVKQGEVLAILEVPEL
                     DAQLQGTQAAVRHSQAQIQRAQGELKREEATHEALHAEYKRLAQAAEVRPGLIAQQEL
                     DDALAKDQSSEAQVLAAKSALEAAQQQLDEAKADNTRVRTLFDYSTITAPFTGVVTWR
                     YADTGALIQAGTSSSTQSMPVVRVAQSDLLRLRMPVPESDVPYIEKGGQVQIHIDATG
                     KTITGKIVRFTRSLDSSTRTMTTEVDIPNPDLSLNPGMYAETTIQLQARRNVLILPAQ
                     AVVQNGNSTYVLALNSQNQIQRRDVTIGLQTPNEIEIDSGLEEGDRIIAAGQSNFQVG
                     QTVRPKFITPLTMPKEESN"
     gene            14913..18089
                     /locus_tag="VFA02_03935"
     CDS             14913..18089
                     /locus_tag="VFA02_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU09027.1"
                     /translation="MSSFAIRFPYFILMMCLIILVVGVTAVTRMPVDLFPQVNIPVVV
                     VATFYSGMPPQQIESDITDTYERFFTLGSNIDHIESRSLPGVSLIKIYFQPGTDADTA
                     TSNIANLAMANLRRLPPGTLPPVVLKYDASNLPVCLITLKGRGLNETQLKDLAQFTVR
                     NQVANVPGASVPQPYGGRYRQIMVYVDPLKLEAHQMSVMDVVQAVNKSNLILPAGDVR
                     IGPKDYNIYTNSQVPVVDEINDMPLKTVGNANVLVADVGKAVDAGQLQTNIVRVDGQR
                     SVYIPVLKQGGNSNTITIVNGIRKAVANLLDVPKNLRTDVAFDQSLFVKMAVRNVIRE
                     GAIGLVLTALMILLFLANVRATISVLLSIPLSVLATFLAIDVWGVSINTMVLGGLALV
                     LSRIIDNSVVVLENIFRHMEMGKPPEEASEKGGKEVQLAVLAATVATSIVFSPVVLLY
                     GVSKYLFTALALAVVLALFASYFVAMTVVPLFCARFIRLGPEHTHDGSHKDVEEDLEA
                     SIDTSAKHHFFHRVVRGFNTVFLKMQEKYDASIRWCLLYPWKVTVAISLFVLLTIGLY
                     PFMGKAFFPRTDPGQFVVNVKVPAGTRIELTDEYVARMENDIRSIVSPEDLNMIVSNI
                     GITPDLSAIYTSNSGMHTAFIQVNLKQEHKIGSYEYMAKVRRRFATDFPEVSAYFQAG
                     GLVDSVVNQGKPAPIDIQVRGNSLRQSYAMAQEIARRVRGLSTVSDVLVPQDLDYPGL
                     ELNIDREHAALAGVSPQSVVDNVITALTSNGMIAPSYWIDPKTGNSYMLTVQYPETQI
                     QTLNDFKEIPLRSPDNGQTTPLESVVDIRQINTPTEVDHYQLRRVFDVYVMPKSEDLS
                     RVNSDVNRVLAGIDKPEGIIIAVEGTVRDMQESFSSFGLGLILAVVLVYLILMAQFAS
                     FSDPFVILLAVPPGISGAILLLMMTGSTMNVMSLMGVLMTTGIAVSNSILIVEFTGTL
                     IKDGMSLRDALREACKVRLRPILMTTLATLLGLIPMALALEPGSEQYAPLARAIIGGL
                     AVSTVLTVYIVPCVYLLIHRGEEKEVTA"
     gene            18086..19462
                     /locus_tag="VFA02_03940"
     CDS             18086..19462
                     /locus_tag="VFA02_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09028.1"
                     /translation="MKNLPVLVFFFASCALAQSSLPSAPQVSLTFSRSPMLSSAQSLP
                     NQTGGGPVRLTREQAEQIALKNNPRVSVAQLLAMAQHQVVRETRSAIYPDVSGNITAV
                     QAEEASRISSGALTASRLLQHAGLGVELSQLITDFGRTHNLISSAKLTEKSQQASAEA
                     TREQIVLATDFAFYNALEAQATLNVANQTVKERQAVEDQVNQLTKTKLKSDLDLSFAQ
                     VNLSQAKLLQLEAQDNYDTAIASLTTVLGSDTHQKYELIDDTTQLPSPPPDENSLEAQ
                     AMQQRPDLQAIDLKHQADQKFARAQWQQLLPTISALGVVGKTPVGSSQYFIGDWYGAV
                     GGNLNIPVFNGFRYTAQAHEAYLRAKASAEQIRDLRDQIVRDVQTAWLRANTAYQKVV
                     VQQELLREANMGLDLAQTRYQLGLSSIVELSQAQLQQTEAAIGDANARYQYQFAISTL
                     NYQIGVHP"
     gene            complement(19470..20804)
                     /gene="nhaA"
                     /locus_tag="VFA02_03945"
     CDS             complement(19470..20804)
                     /gene="nhaA"
                     /locus_tag="VFA02_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZU09029.1"
                     /translation="MTIPARTLGELKLQVRESLITRALQLPIQQFIHTQGVSSAFLLA
                     AAVVALIWANSPWSASYYHIWHIHLTLSGLELPVHEWINDALMALFFFLVGMEIKQEI
                     VHGELKDLRRASLPICGGLGGMVLPAMIYAALNYGRPGAHGWGVPMATDIAFSLGVLA
                     MVKGVPAELKVFLLSLAIADDIGAIAVIAIFYTDTLHLNYLLMAALLVTIILLCRRIG
                     IRRQIFYAALGFGVWLAILRSGVHATIAGVVLGFLVPVTSSVSLDQFGDVCSEVLEDF
                     KKARASGDNSLANRQLGAMEYLIANTESPAERITRKLHDWIAFLVLPLFALSNAGVTF
                     SLTTWKSLLHSPLAWGVLLGLLIGKPLGITAACWIAVKLKIAQLPGKVKGAQIVSVGT
                     IAGIGFTVSLFISALAFADPAQLDAAKTAVLTASLLAGVSGYVLLSRTTQKE"
     gene            20857..21105
                     /locus_tag="VFA02_03950"
     CDS             20857..21105
                     /locus_tag="VFA02_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09030.1"
                     /translation="MLLMLAPLRFLWNATRGHRFSPWRSEYLKWRIETYSGKKAETLQ
                     SRDVIAFLWQQKWILLRFLRWTGEMDSFRKISSRVNEE"
     gene            21108..22208
                     /locus_tag="VFA02_03955"
     CDS             21108..22208
                     /locus_tag="VFA02_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HZU09031.1"
                     /translation="MRNATRREFLKGAACLGLAGLMPLSAEKDIGDPTSLRTHAEARG
                     LLVGCAVNPEKLRDEPLYAQTVAEQESLLVAENAMKWPALHPAPDKYNFAPADSILAF
                     AEAHKQQLRGHNLCWHEALPDWFRSVVTKENANHVLAEHIRTVAGHFAGKLHSWDVVN
                     EAIDPKDGRPDGLRKSPWLELAGPYYIETAFRAARQADPKALLTYNDYGIELDTPDQT
                     AKREQVLALVQRLKKRNVPIDAVGVQSHLTAGDPTPGAGLRDFVRKLRSMDMQVFITE
                     LDVSDHKIQGTIAERDAAVAKLYGDYLRMMLAEPNVKAVLTWGITDKYTWLNHQHKRS
                     DGQPQRPLPFDAEYKPAPAFYAMRAAVDSRPS"
     gene            22244..22573
                     /locus_tag="VFA02_03960"
     CDS             22244..22573
                     /locus_tag="VFA02_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZU09032.1"
                     /translation="MFNPLKIQEMMAQAKQMQEQMQEKLGQTIVEASSGGGAVTVKMN
                     GQKQVLKVTIDPSAVASLSGNANDVEMLEDLIAAAFNEAGRRADEAIKSAASSMLGGL
                     NLPPGLL"
     gene            22752..23702
                     /locus_tag="VFA02_03965"
     CDS             22752..23702
                     /locus_tag="VFA02_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU09033.1"
                     /translation="MLTRRVFLKQASLAGAAWMTAPYMSRGQQTSVSNDRIRQFREGG
                     ATTPIHVTNLRDNLYLLQGVGGNMVVQTGPDGTLLIDSSASTAAPKIEDALKKLDAHP
                     LKFLVNTHWHYDHTDGNAALHGSTGVFIIAHENTRRRLSAPQEIAFLKMRFDANPPAA
                     LPQQTFTDNQKMFLNQDELRLGHMPAAHTDTDIYVHFVNGNVIHAGDIFFNGLYPFID
                     GSTGGTFHGMMRAAETCLALADNETKIVPGHGPLGDKASLQKYHDMLSGLAERVGKLK
                     ASGKSREEVIAAKPTADFDAEWGKGFLLPDQFAGLVYDTL"
     gene            23756..24568
                     /locus_tag="VFA02_03970"
     CDS             23756..24568
                     /locus_tag="VFA02_03970"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZU09034.1"
                     /translation="MFKIGEFSALTRISVKTLRFYDEVGLLKPAHVDTWTGYRYYSAQ
                     QLPRLHRILALKDLGFSLEQIVKALDSNISPQELRGMVMLRRAEQEGRVREEQERLNR
                     LETLIKFIEQESVMSADVVMKEVPPQWIVSVREVIPNYPSVGMLYGKVMGQIGGGCNL
                     GMPVAIWHDLEHKEKDVDAEAGFLLKEPLAAHGTAQMYQLQPALVASYMHHGAYNRLD
                     QAYQNLLRWVEENHYKLTGPFRELYHYCAQPVRQDDESYVTEIQIPVEKREG"
     gene            24656..>24888
                     /locus_tag="VFA02_03975"
     CDS             24656..>24888
                     /locus_tag="VFA02_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HZU09035.1"
                     /translation="MARLIDELRKLPGIGAKNAQRLAFHILRSSHEDAEALADAIHSL
                     KEKLQLCSVCNNITDMDPCVYCSSPTRNQRLVCV"
BASE COUNT         6140 a         6103 c         6613 g         6032 t
ORIGIN      
        1 cgcaatccgg agcttctggc atccgtggcc ccgggaacaa ctttgacggg gctgcgccag
       61 cagcaaagcg aattgatggt ccaatatgct cagttgaagg cgaagtatgg tccggaatgg
      121 ccaaagctaa aggaagcgga aaaacagctc gcgaccgtgc agacgaacat agatgccgaa
      181 atcaataatc tgacgaataa gtttcaggaa gaatataacg ctgctgtaaa tacagaaaac
      241 cttctacgct ctcgcctgga ccagattaag caagaagcct atcggacaaa tgcatctgaa
      301 gcggagttcg acatcctgaa acatggagct gactccgcct ccgacctgta cgatgcgttg
      361 cagaccaaat tgaaggaagc gggtattacc gctggcctga attcaaacaa catagatgta
      421 attgacaggg ccacagagcc agcacgtcct gtctctccta aaaagagaat agatcttgct
      481 gtggggttca tgggcgggtt gttgatcgga gtgatgattg ctctggtcct tgagtcgctg
      541 gatgatactc tgcggacatc agaagatgtt gaagctgtct ctggcctgcc gtctctggcg
      601 gtgattccgc attttacttc tatacgcaaa atcgcctcat cttctgccga tggggccgct
      661 gcgatggata aaaccggagt ttctgccgag attgtttcct atacagagcc tcattccata
      721 ggagcagaag gatatagaac tttacggtct tcgatcctgc tttcgtctgt ggatcgagag
      781 ccgaggctta ttttgctgac cagcagctat gcggcagaag ggaaatctac ctgcgcagca
      841 aacctggcga tttcattcgc gcatcggtca gcccgggtgc tcgtagtgga tacagacctt
      901 cgcaagggga cgctgcacct gaagttcaag acatcaaacc gtgcaggact gtctacgctg
      961 ctggcgcgtg aatcaggcga agaagcatac gagcggccat tgcctgatct gccaaacctc
     1021 gtagttttga cacgcggacc gatagcaccc aatcccggag agatgctggc atcacgcagc
     1081 atggaagacc tgctgctgca atggcgcctg gaatatgatc atgtgattct ggacacttct
     1141 cctgtactcc ccgttgccga tacgttgagc cttgctccgc acgtggatgt tgcgtttgtg
     1201 attgtccgcg ccggcattac acgaaagaag gccctgctga aagtacgcga ccagctgcga
     1261 agagtgaatg caagactcct gggaacagta gtgaacgatg tggaccttcg tctggagaac
     1321 tactacacgt attccaaacg ctatgattat ggctacaaag gatatggagc aggctacgga
     1381 gtgaaggatg cgcaagatgg cgaaaagtaa agcaattgtc ctgtcgcttt ttgtatttgt
     1441 atccatttgg cagtgccggc ttctcttcgg acaaaccgct tcgcaggaag cccagatacc
     1501 gcaaagcgca cagtccccta gcatcgcaat cggcccaggt gatcttctga gtgttgtcgt
     1561 ttttgatact cctgagttga gcacttcggt ccgtgtaagc caggatggcg aagtcaatct
     1621 tccggtactc ggacgtattc atcttgccgg agtgaatacg attgatgccg cgaggaaaat
     1681 cgagcaggaa ctgcgcaccc ggcgccttct gcttgaacca catgtgactg tttttattgc
     1741 cgaatatgca tcgcagggcg cgaccgtcat gggagaagtc aggaccccgg gagtgtaccc
     1801 aacgttagga acacgcaggc tcctggacat gatttcggtt gcaggcggat tacagccaac
     1861 cgcgggcaaa acggcgtcca ttattcatcg ggatgatcct cagcatccgg tcaatattac
     1921 gcttcaatcg cacccgggtg ctttatcggc gcaggaaaat ccggtgattc ttcctggaga
     1981 cacgatcgtt gtggcaaaag ccggtattgt ctatgtcatc ggagatgttt tgaaacccgg
     2041 cggttttctg atagataaca atactccgat ctccgtgatg cagtcgcttt cgctggcagg
     2101 aggatgggac aagaccgctg ctttgagcaa ggtcaagctg attcgcaaaa ctccggaagg
     2161 aagggaagaa gttgatcttg atctcaagca tgtgacaaat ggatcacagg ctgatttgaa
     2221 ggtgaccgat ggcgacatta tctttgtgcc ttccagcgtg ggaaagacca tggcctatcg
     2281 tggagttgaa gcagccatat caacggccac cggtatagca acatatggcc atatttatta
     2341 aattccaaga tcgccatggc ttaccagtgg agccctcatg atgtatcttc agcggcacac
     2401 gatggattta accaaaaaaa ttgtcctcgt actgagtgcg gtgacggcca tcgccacagt
     2461 gtcgcaaatt gcgctggggg ctgatttcgt cattttgatg atggcattat gtgccattgc
     2521 atcgggactg gctggttttt accttctggg ttcttacaat ttggtgggct ggtggtttct
     2581 gctttacgtt atcgggcatg ttggagtggc cctggttgcc aagactctgt acagacagcc
     2641 actggagtct tacctatacg caccatttca ttcctacctg gtagaagctg ttacctgctt
     2701 tatcgcgctg tgcgccatta ttctggtgat atggacgaat acaggaaagc cgctgtttga
     2761 gaaaataaca gatccgggac tgctgctgtt cttatcctgg agctgctttt ttatcggttc
     2821 ggctgttatg ttattgttcc gcctgattgc aggtggagac aagtctggtg gagccagtta
     2881 tggcggagtg gcctttcttg aatctcttgc ctatatggga atcatcgcaa ggacggcata
     2941 tcttgcgcag gcgagtcacg gtaaaaagtt cttagattct aagcttttat tcatgctggt
     3001 tgccttaaca ggactcggca ttttggctgg tggaaaaagt tctactgctg tcccatggct
     3061 ctgctttctt ctgacggtcg ttttttttgc aggcaaactg ccatggcgtt atgtcggctt
     3121 catgttagca ggcggccttc ttttcgttct ttttttgtct ccctttattc atatacttcg
     3181 cgggctacag ttgcgcgatg caagtctggg cgaggaaata agtttgatgg agcattacct
     3241 tccattgata cttaaccctg atgagttttc atttttcgcg caaagagcaa gcaccgatac
     3301 caattacttt cactattttg gaagcccgaa ggaaggcata ttgctgccgc gttttacatc
     3361 tgtgcagcag attgacccag tcattgttgc cagagatgcc ggtgacagaa tggatacaaa
     3421 gctggcgctt gatgcggtca aggcaaactt accgaagttt cttgttcccg ataaacccac
     3481 tgaaacaaat gcatacacta ttttggtgca tgaaggatac gttccggtat attcaaccgg
     3541 gcactatccc acattgcctt acagtggaga gatatatctc atctttggat ggccgggcgt
     3601 aattgcaggg tcatttgcgc tgtttttcct gtattttctg gagttaaaaa aaattggctg
     3661 gactttatac tgcaatatat ttgctgtttt cttgctcgga tcgagcttgt atcacagtaa
     3721 tgaaatgtcg ttgcaggatt tcattgggca aatcatccgg gattttccaa tatttgttct
     3781 tctgattatt tttctggttc gtgcatacta tgtgttctca aggagaattc tcacgaaaca
     3841 ggaaagctac gctgagacat gattctattt gtgctagtta taagcggagt tattcctgat
     3901 ttatggagga ggagaaatga aaacaagccg gtatgcagtc tacttgctgt ttatggccat
     3961 cttgctgatg tctgccccct cctttgccca attggattct acttcggtgt ccacaccaat
     4021 ttcatcgcct gttggtggtc ctctaagtga aagcaattcc agctttgctg caacgacttc
     4081 aactgaaatt gaaaccggcg caagcaatct cctcaaccct gacgtgcgtg atgcaatgac
     4141 aaccgattca gtaggcgcgg atgtttccgt tactgctatg aatccgtcgc ttgaaaagtc
     4201 agtcatggtt ccgccggttt tgtcgctgcc agatacatat gatgagcagg atgccttgac
     4261 gctcaccgga gcgcgtcggt acagcgtgcc gccgttgttc ggcgttctac ctccgacacg
     4321 tccggctaat acgcctgaag cactggcctt ttcgcatgta actttaccgg tatcgcggtt
     4381 caacagcact gcttttcgag tgtcggtgaa ttccataaat gccaacagtt tcgatccggg
     4441 cgtcaaagca ggtcagacgt ttactggaaa ccatgtccag acgccggcat ggggacaatc
     4501 ggctttatcg gaaaatggaa tgcctgcgaa tctcccgtcc ggggtggacg tattttcggc
     4561 gcagcaggtt ccttcatatc cgcaaagtgt ttccgcgcaa aagagagttc aaaggccgca
     4621 aaacaatcca ctgtctttgg gccggcagca acaattgtca gagcaatccg gtttctctcc
     4681 cagatacgtt gtcaggaatc tatctatggc cgctgtgggt acttctaacc agctgacgcc
     4741 aggtagtatg gactggtctg cctggaacac atggccccag gatggttttt taaatggcac
     4801 ttctctcttc aggcttaaca cgctgggcga gggcaatgtt ttttctcccg atatcttcaa
     4861 tccccggcta aaaggaaatg tgagagatcg cgggggcaat aatcaaatgc gcattgcgag
     4921 ggagcaggcc aatcgcattt tgcgctttag cgagactaaa tctgttgatt ctcaaacatc
     4981 gaatagactc aatcagacat atacgctatt tccgcacacc agacgcagcc agacgcaatt
     5041 caaacgttac cgttatcata atccgctttt gaataatgac gttagcagtc aatacacgcc
     5101 ggactctcaa tactgaatga ttgaaaatct gatgaaagcc aggatatcac ttcgttttca
     5161 gtgcgaatcc ggttacaata aagtcctgcc tgctagatag agcaaatcaa caaacttaga
     5221 ccatcactag ctattctctt ctgttctgcc tgataagttt ttttgatgat gagattcgaa
     5281 tgaaccatgt tccaactttt tctgagagct taaacttatg aaagaacaat tgacgattgg
     5341 tattgtaggt tctggatacg tggggttagt gactgcggtg tgttttgctg aactcggaca
     5401 ccgcgtcatt tgcgtggaca gcgacgaaaa gaaaattgct cttctgcgtc agggaaagcc
     5461 acccatccac gaggaatatc ttccgcagtt gctggagcgg cactgtaacg gcgccattga
     5521 atttaccacg gacctgaaaa gtgccacgca gcgctcccag gcgctcttca ttgcggtggg
     5581 cactccgcaa agccaggccg gcaatgctga tatgtcctat gtggacgctg tggcgagcga
     5641 aattgcccgc gccattacca gctacaagct tgtcgtggaa aagagcacag tcccggtcta
     5701 caccaatgaa tggatcagcc gcatcatgat gcggaatggt gtgccccgcg atctgttcga
     5761 tgtcgcttcc aacccggaat ttttgcggga aggaacggcc gttgtagact tcctccaccc
     5821 tgaccgcatt gtgatcggcg ttgacagcgg ccgcgccgct gatgtccttc gggcggttta
     5881 tgagccgctg acttcaggaa actactataa acaggctggg gcggtcccag gaccacgcat
     5941 ggcttctggg cttccaccgc tactagagac ttccactaaa gcagcggaaa tcatcaagca
     6001 cgcatcaaat gctttcctgg cgatgaagat ttcattcatt aatgtcgttt ccaacatttg
     6061 tgaggcggtc ggagctgata ttgagcaggt ttccaaaggt atcggatcgg acggcagaat
     6121 cgggcataaa ttcctgaaag cgggaatcgg gtatggcggc tcctgttttc caaaggacgt
     6181 tgctgcattc cgttatgtgg cggagcagat gggcattgaa tttgggctac tcagtgaggt
     6241 tgagaaagtc aacacggagc agaaacagcg gtttttccag aaggtccgtt ctgccctctg
     6301 gacattcaga ggcaaaaggc tgggcgtcct gggtttggca ttcaaaggcg gcacggacga
     6361 tattcgcgaa tctccagcca ttgatttggt gaggatgttt ctgcaggaag gttgcatcat
     6421 ctctgcctat gatcccgctg ccatggaaaa agccaaacag gtgcttcccg catcagaaag
     6481 cctttccttt gccaaggatg cctatgaggc ggccgcagat gcagacgcgc tgctggtctt
     6541 gacagactgg gctgaattcg ccgagcttga cctcgaccgg ctgtcccgta ttttgcggta
     6601 tcccgttgtt atagacggtc gtaatctgtt tgatcctgca ttaatgcggc agtatggatt
     6661 tacttatctg ggtattggca gagcagccgg ggcccatgaa aaggacccag cctttgctta
     6721 taaattaccg taattgtaag tggtaactat tacttatgta cttatagaca aacctattca
     6781 cacgtagtgt agtaaataac agattatcgc catacctgcg atttctgcag atgtggccat
     6841 atcaagggga gccgagaaga gatgaaactt accaatgaaa aagttgtagt ttgcggggca
     6901 ggtggattta ttggagggca cttggtcaag gcccttctca accagggcgt aaacgttgtg
     6961 cgtgctgtgg atgtgaaacc attggacgag tggtatcaga agtcaaaaga agtagagaac
     7021 ctcgttcttg acctgcgcga taaggacaac tgctataagg ccgcaaaggg aatgcaggtc
     7081 gttttccagt tggcggctga tatgggcggc atgggcttta ttgaaaacaa taaggcgctc
     7141 tgcatgctga gtgtgctgac caatacgcat atgcttatgg cggcaaagga ctcaggcgta
     7201 gagcggttct tctattcctc ttccgcctgt gtttacaacg gcgataagca gaaatcgccc
     7261 aacgtcgtgg cgctgaagga agaagatgcg tatccggcgc tgcccgaaga tggttatggc
     7321 tgggagaagc tgttcagcga gcggatgtgc cggcacttcc gtgaagactt tggccttgaa
     7381 actcgtgttg ctcgctacca caacgtttat ggcccgttcg gtacttatga gggtggacgt
     7441 gaaaaggccc ccgcagcgat ttgccggaag gtgattcagg ccaaaatttc cgggaagcat
     7501 gagatcgaaa tctggggcga cggccatcag acacgcagct ttatgtatgt ggatgattgc
     7561 acgaagggaa cacgggccat cctggaaagc gacgatattc ttgaaccgat caatcttggc
     7621 agtagcgagc tcgtcaccat caatcagctt gtggacatcg tagaagaaat cgcaggcgta
     7681 aagctcaaga gaaattacaa gcttgatgct ccaaagggcg tgaatggccg caacagtgat
     7741 aacacaaaga tactgaagta tctcggatgg gagccttcaa ttcgtttgcg cgacggtatg
     7801 cagaggacct atgaatggat tgaaagccag atgctggcga ctgttgctcg ataaaaaaaa
     7861 tcagaaggtt tgcttttggc agacacgcag tcatttgaaa gcgctggtat cggtgggcct
     7921 gtaaatcggg ttttgattta caggctgggc agtctggggg acacggttgt cgtgcttccg
     7981 tgtctccacc tgattgccag agtgttcccc aatgcgcggc gaattatgct tactaattcg
     8041 cccgtgcatg caaaggcccc cgcaactgca tcggtaatag gtagttcggg tcttgtgcac
     8101 gattacatca cctaccaaac gggatcgcga agtcttgctg gcttcgcccg tatctggtgg
     8161 aaaatacgag aattttcacc agatattttg atctatcttg cagcgcctcg cggcgacaaa
     8221 gctatacagc gcgatctttc ttttttccgg ctgtgtggca tccggacaat gatcggtgcg
     8281 ccggttggag aactggctga accgctctac gatgctcaaa ctggtctttg ggaacatgag
     8341 gcattccgcc ttgcccgcac cctggctcca ttgggaaatg tagatctgga gaacagcgag
     8401 agctgggacc tccggttgac gcctgcagaa attaacactg caagccgtgt tcttgccggt
     8461 ctgaatggaa agcagttttt ggttgcggca attggcacca agatgcaggc aaaagactgg
     8521 ggtactgaaa aatggcaagc actgatgcaa cggttaggcg aagtttttcc tgatcatgca
     8581 atggtctttc ttggtgccag ggatgatttt gctacctgcg aaaaggcaag atcgaagtgg
     8641 cagggagagg cattaaatct ctgcggtatg cttactcccc gggagtctgc tgcggtcatt
     8701 caaagggctg aattgctaat gggaccagat agtgggccta tgcacgttgc agcagcttta
     8761 ggcgtaccat gcgctgctgt atttgccgcg agggcaagac ctggtcattg gtttccgttc
     8821 ggaaaaggcc accaggtgat ttatcacaag accgattgct tcaattgcaa tctggaaacg
     8881 tgcatcgaac agcgtaagaa atgtattgaa tccgttcagg tagatgaaat gctgaatgcg
     8941 gccgtcaaag catgggagta tggaagaaga aagcgggaga tgcagcttgc ataaaggcaa
     9001 aaagtgggga ctgcggcctc ataaaagaaa ctgctcaact gctgtaagca aagagtctgc
     9061 gactgcagta gccagggaag ccgctttttc tgcccctggt ttttgatgtt tcggatctcc
     9121 cttaataaat atggtaggca tgccaagtgt gatgcccgct tcgatatcag agagagagtc
     9181 accaatcatt acgctgttat gagaggatgc ggaaggaaaa tcgagaaatg cctgctcaaa
     9241 tagagctgta cccggcttgc ggcaacggca ttcattccta tcatgcgggc aaaagtaaat
     9301 tgcatccaga tgtgcgttga agcaagtaag atgctggttt aatttttcgt gcagatgcag
     9361 aacgtcgtct ttactgtaga gccccaatgc aattccgcgt tgattgctga taataatggc
     9421 tttccgtccc gagtgattga gcattgcgat ggccttttcc gcgccgggca gaatgtgaaa
     9481 atcatcccac tttgcaatat atttaccctc gggaggcttg cggttgatta cgccgtccct
     9541 gtcaaggaag acgtagcgca catttgcaaa cttgtcctgt tcagcaacgt tctgcatcat
     9601 tttaatcata cccgcctgaa ggcagagcca tcggaaaaag cgggaactgt gctgtatcct
     9661 gagaatcggc tatgaccata gacaatgctt cagtttttaa gggtgccatt cgcgagcatc
     9721 tttccgtagt acaacaggtg gaggctctga tacccgccct tgaacgcatt gctcgcgaaa
     9781 tgctgcgttg tgtaagcggc ggaaataccg tcctctggtt tggcaatgga ggcagcgcgg
     9841 cagatgcaca gcatcttgcg gcagaatttg ttggccgttt ccgtcgtgaa cgctgtggtt
     9901 tgccttccat agctttgacc acggacacct ctgttctgac ctcgattgcc aatgactacg
     9961 gttatgaaga tgtattccgt cgtcaggtcg aggcattatg ctctacggga gatgttgcag
    10021 tcggcatctc gacttcagga aatagtaaga atgtttgccg tgctttggaa gaagcccgca
    10081 aacgcggcgc atttacagtc gccatgactg gagaaggtgg agggttgctc gcttctattg
    10141 cggatgcaat cctttgtatg ccctcgcgcg ataccgcgcg catccaggaa ggccacattc
    10201 tctgtgggca tatgttgtgc gattgggtgg aaatcgctat ctgccagcag gcagcacatc
    10261 agaaaggcca gcaatcatga ttaccgacct gcatcgggtg gttcaattca ttgaaggcga
    10321 ctggaagaac aagcgtgttc ttgttgtcgg cgatgtcatg cttgaccggt atgtttgggg
    10381 cgatgtcgag cgaatttccc ctgaagcccc tgttcccatt gtccgagcag gtcacaaaag
    10441 cagtcgggct ggcggtgctg caaacgttgc tgccaatatt actggacttg gggccggaag
    10501 cacccttatt ggggtaatag gagctgatga agatggcgaa tgtctgaagt catgtctaga
    10561 cagtatgggc gtaatccata agctcgtggt ttcacaaaag cgtcctacta cggccaaact
    10621 acgggtgttc ggcgggaatc agcagatgct gcgtctggat gtggaggaag tgacggagtg
    10681 tgacggagaa attgaagagc aaatactgca gcatgtaaag caagtgctcc ctgctatgga
    10741 tgctcttgtc ctttctgact atgccaaagg cgtgctttca gagaaggtct gccaatcttt
    10801 gattgcatcg gcgtatcgcg cgggtattcc tgtgctcgtt gatccgaaac agcgcagttt
    10861 cgaacgatac caaggggcca ccacaatatg ccccaatctg caggaactat ccaccgcaac
    10921 cggagtttcc tgcaaaaata tcgagcagat actgagtgcc gggcaggaac tagtagcagc
    10981 gcttaaattg caatatctga cagtgacatt gagcgaaaag ggaatcgcta ttttacgcaa
    11041 agacagcaaa gatagttttc ctgctatggc gcgccaagtg tacgacgtgt cgggtgctgg
    11101 cgatacagtc atagcaaccc ttgctctctc tcttgccagc ggtcttgaga ttgaaactgc
    11161 ggcccaattg gcgaacgttg cggccggaat tgtcgtgagc aaggttggaa ctgttcctgt
    11221 agagcgcgat gaacttcttg ccagccttat gcctgagatt gaattgcacg ccgaagaaaa
    11281 agttctgagt ctcgaccggc tgaaaacgcg cgtttctgcc tggcgctcgc agggggagaa
    11341 gattgttttt acgaatggct gcttcgattt gttacacatt ggccatattc ggcttcttca
    11401 ggatgcgcga agagaagggg accgcctcat tgttgccatt aacagtgaca aatctgttgc
    11461 cgaattaaag gggccaacac gtccgattgt gggagagcaa gagcgagcgt atgtgcttgc
    11521 tgctctcagt gttatagacg cagtggtgat ctttggcgaa cctacgccat tgaagctgat
    11581 cgaagcactt cgtccagacg tgattgtgaa aggcggagat tacacagaag aaaccattgt
    11641 cggggcgcgg gaagtccggt cctggggtgg aagggttaaa attgtgccca cggtaccagg
    11701 cttcagcaca acgaaattaa tcgaaaaggc aacaatcgaa gcatgatgga ttgaagcagg
    11761 aaaagtttat gatgatacac tgaaattaca gccattctac tcggagaggt ggcagagccc
    11821 ggttgaatgc acctgactcg aaatcaggca tacctttacg ggtatcgggg gttcgaatcc
    11881 ctccctctcc gccatctcta taaattgcca caatataatc aacaacttaa aggcattaag
    11941 tctgaaaacc ccagtaaaac cagcatgatt atcttgcagc cgtatgcaaa gttaacgtcg
    12001 gtctgcgaca aattacgaca tggaatctat aaagtttgga taccatagat accacgtctt
    12061 ctgctatcgt gttgctcatg cgtacccctt ccatcaatct caagttcaaa acgaaggtca
    12121 atgaaaagtg gacgtttgtt ccagtggccc gcgcaggcgg aaagtacgtt ccggatcggg
    12181 ttgtcatcca cggcaaaaca atcaaagcgc cagaaggctc ctattacctt gagtggtggg
    12241 aagtcctaaa gcggaggcag agagcggtcg gagcaaactg gcgacacgca atggatgcgg
    12301 taagaatcca gacgcacgtt ctggcccttc gcgctgaggg agcagaagac ggaaccagtg
    12361 gacctgaaaa atctttgccc agagacaatg gccttacttt ttgaggattg cgcgattgct
    12421 tcccgatccc gctatttggt gagacgggct ttcatgcgaa gtccgtcttt cctttccagc
    12481 aaagttacca cctcatcatt tcccacagtt tctgctttaa atagtttttt catatgtgga
    12541 gaactgacag atatcttgac ggtacgtaat ggatatttgc caagatcggc cccgcccgca
    12601 agaagcgcgt ccacgatatc atgaaggctt gcgctgccaa cttctcgcag atactcacgt
    12661 atggcgaccc aggtacgaat tcctctgtat tttgtccgca ggcttccatc cggagccagc
    12721 gcatcatagg ccttttgcag agaggctagc tttgcttgtg ttgcttcaat ctgctcttgc
    12781 agattgctga gcagcgattc aagtttttga tcttcagcca taaagattgt tcctcgtttt
    12841 aaacccttgt ggttttttaa agcgatatac taaaccggat acattgcagg ataaatagta
    12901 agaacttctc tgcaaccagt taattttact atgctaacat tacgagagta taacgaagtt
    12961 tcttttattt cgaattgaaa gatgtacaat tggatgagcg aatagctggt cgattcgata
    13021 ccaaaataga taccaagaca ataatgaaaa gtgaaactat tgaaaagaat agacttgtga
    13081 ttgaaaaggt tcgaatccct ccgtctccgc cagagttgtt acgatacgtt ctttcttagt
    13141 atgcgtccaa acagagtagt ggtgtaagcg agattatgca aagttctgca aaacggcaat
    13201 tttctatgtg gctgattgca gggctgtttt acctttcctg cgctgtattt gcatgggggc
    13261 tgcaatataa gctttctctg tatcacaatg cagacgggtc cgcggaggtc ccggtggcaa
    13321 agctattcgt cagctctgac aaatcttctt tgcagcgcag cgtcagtgat gatgcacagg
    13381 ggacattgac cagccagtcg tttttatttg ttgtctcatt tcttgcattt ttgtcgctga
    13441 aatttgcagt agattatcgg gcccagcaat acaaggtaat tcagagagct ttccttattc
    13501 caggaagact atcgctgcag agacctttct tttttcgccc tccccctgcc ttcatccatt
    13561 aatttactca tgcttgtttt taacaacagt cctccggcac tgttgttacc gttgcgtttt
    13621 gagtatcaag cgcatccggt cgtcacaacc tatctacggg acaggatgtg agtataaatg
    13681 gttaaattga tttcttatgg aaggcaattt tcagctttag caatctcttt ttgtcttgcg
    13741 gcgactttgc ctggatgccg ccgaacaaag gatgtgaagg catccacgga acccacagct
    13801 ccggttgttg ctgttaagca aggagatttg gcaacacatc tcgtcgtggc gggtcagttt
    13861 cagccgtatc aggaggttga ccttcatgcg aaggtgtcag ggtatattcg tgagatccgt
    13921 gtagacattg gggaccgggt aaagcagggc gaagtcctcg ccatcctgga agtgcctgaa
    13981 cttgatgctc aattgcaggg gacgcaggcc gcagtgcgac atagccaggc acagatccag
    14041 cgtgcacagg gtgaactcaa gcgtgaagag gccacgcatg aagcgcttca tgccgaatac
    14101 aagcgtcttg cccaggctgc agaggtaagg ccgggcctga ttgcgcaaca ggagctggac
    14161 gacgctctgg ccaaagacca gtcctctgaa gcacaggtcc tggcggcaaa atctgcatta
    14221 gaagcagcgc aacagcaact agacgaggct aaagctgata acactcgtgt ccgcacgctg
    14281 tttgattact ccaccatcac agcgcccttt actggtgttg tgacatggcg ttatgcggat
    14341 actggtgcgc tcattcaggc aggtacaagc tcatctacac aatccatgcc tgttgtgcgt
    14401 gtggcacaga gcgaccttct ccgactgaga atgccagtgc ccgaatcgga tgttccatac
    14461 attgaaaagg gcggccaggt gcagattcat atagatgcaa ctggaaagac gatcacagga
    14521 aagatcgtcc gcttcacccg ttcgcttgat agttccacgc gcaccatgac gactgaggtg
    14581 gacattccca atcctgatct ttctctcaac ccgggaatgt atgcagaaac aaccatccag
    14641 ttgcaggccc gcagaaatgt gctgatcctt cccgcacagg cggtagtcca gaatgggaac
    14701 agcacatatg tacttgcact gaatagccag aaccagattc agcgacgcga tgtgaccatt
    14761 ggactgcaaa ctccaaatga aattgaaatt gacagcggtc ttgaggaagg ggaccgtatt
    14821 attgctgcag gccagtcgaa tttccaggta gggcagacag tccggccgaa atttatcact
    14881 cctttgacga tgccgaagga ggagagtaac taatgtcctc ctttgcaatc cggtttcctt
    14941 atttcatttt aatgatgtgt ttgatcattc ttgtggttgg cgttacagcg gtcacaagaa
    15001 tgcctgtaga cctttttcct caggtgaata ttccagtcgt tgtggtggcg acgttttatt
    15061 caggaatgcc gccgcagcaa attgaatcag acattacgga tacctatgag cggttcttca
    15121 cgctgggtag caacattgac catatcgagt cccgctcact gcccggagta agcctgatca
    15181 aaatttactt tcagccaggt acagatgctg acacggcgac gagcaacatt gcaaacctgg
    15241 cgatggccaa tctacgaaga ctacctccag gtacgttgcc tcccgttgta ttgaaatacg
    15301 atgcgtcgaa cctgccagtc tgcctgatta cccttaaagg cagaggactt aatgaaacgc
    15361 aactgaaaga cctggcccaa tttacagtac gcaatcaggt ggcaaacgtt cctggcgcct
    15421 ctgtccctca gccatacggt ggccgctacc ggcagatcat ggtgtatgtt gacccgctca
    15481 agctggaggc acaccagatg agcgtgatgg atgtagtgca ggccgtcaat aaatccaacc
    15541 tgatcctgcc tgctggtgac gttcgcatag gccccaaaga ctacaacatc tataccaaca
    15601 gccaggtccc ggtagtagat gaaattaatg acatgccatt gaagacggtc ggcaatgcga
    15661 atgtgctggt tgccgacgtt ggcaaggcag tggacgcagg acagcttcag acaaacattg
    15721 ttcgcgtgga cgggcagcgg tcggtctaca ttccggtgct gaagcagggt ggcaactcca
    15781 acaccatcac gattgtgaat ggtatccgaa aggccgtggc caaccttttg gatgtgccaa
    15841 aaaacctgcg aaccgatgta gcgtttgacc agtcgctttt tgtaaagatg gcggtcagaa
    15901 acgtcatccg agaaggcgcc atcggtctgg tcctgactgc gttgatgatt ctgctcttcc
    15961 tggcaaatgt gcgcgccaca atctccgtgt tgctttcgat cccgctatcg gtattggcca
    16021 cgtttcttgc cattgatgta tggggcgttt ccatcaatac gatggtactg ggcggtttgg
    16081 cactcgtttt gtcccgtatc attgacaatt ccgtggttgt gctggaaaac atcttccggc
    16141 acatggaaat gggtaagccg ccggaagaag cctctgaaaa gggtggaaaa gaagtgcagt
    16201 tggctgtgct ggctgccact gtagcaacct caattgtttt ttcacccgta gttttgctgt
    16261 atggcgtcag taagtatctg tttacagcac tggcactggc agttgtgctc gctttgtttg
    16321 cctcttattt cgtggccatg accgtggtgc ccctcttctg cgcgcgcttc attcgcctgg
    16381 gaccggagca cacccatgat ggttctcaca aagatgtcga agaagatctt gaagcttcca
    16441 ttgacacttc agcaaagcac catttctttc atcgggtggt caggggcttc aatacggtct
    16501 tcctgaaaat gcaggaaaag tatgacgcaa gtatccggtg gtgtctgctt tatccctgga
    16561 aagtgacggt tgctatttct ctttttgtct tacttaccat cggcctttat ccgtttatgg
    16621 gaaaagcatt tttcccaaga acagaccccg gccagttcgt cgtcaacgta aaagtgccag
    16681 caggcactcg tatcgagctt accgatgaat atgttgcgcg catggaaaat gacatccgca
    16741 gcattgtttc tccggaagat ctgaacatga tcgtttccaa catcggaatc acgccagatt
    16801 tgtctgctat ctatacttcc aactcaggta tgcataccgc ttttatccag gtaaacctga
    16861 agcaggagca caagatcggc agctacgagt atatggccaa agtacggcgc aggtttgcca
    16921 cagattttcc tgaggtcagc gcatactttc aggccggtgg tctggtggat tcagttgtga
    16981 accagggcaa gccagcacca attgacattc aggtgagagg caatagtctg cggcaaagtt
    17041 acgcaatggc gcaggagatt gcaaggcgtg tgcgtggcct gagcacggtc agtgacgttc
    17101 tggtgccaca ggacctcgat tatcctgggc tggagttgaa tatagaccgc gagcatgcag
    17161 cccttgccgg ggtctcaccg caaagtgtgg tggacaatgt gatcaccgca ctgacatcga
    17221 atggcatgat tgctcccagt tattggattg accccaagac cgggaacagt tatatgctca
    17281 cggttcagta tcctgaaacc caaatccaaa cactgaacga tttcaaggaa attcctctac
    17341 gctcgcccga caatgggcag acaacgccgc ttgaatccgt tgtagacatc aggcagatca
    17401 atacgccaac ggaggtagac cactatcagc tccggcgcgt gtttgatgtt tacgttatgc
    17461 cgaaatccga agacctttca cgtgtcaaca gcgatgtaaa ccgtgtactc gcaggtatag
    17521 ataaacctga aggcatcatt attgccgtcg aagggacggt ccgtgatatg caggagtctt
    17581 tctccagttt tggactggga ctgatacttg ctgtcgtgct ggtctatctc attctcatgg
    17641 cccagtttgc atcattttct gatccatttg tgattctgct tgctgtacca cccggcatat
    17701 ctggtgcgat tctccttctg atgatgaccg gctccaccat gaatgtaatg tctctgatgg
    17761 gagttctgat gacaacgggt attgctgttt caaacagcat tctgattgtg gaattcaccg
    17821 ggaccctcat aaaagatgga atgtccctgc gtgatgcgct gagagaagcg tgtaaggtgc
    17881 gtctgcgtcc gatcctgatg acgacgctgg caacactcct tgggttgatt ccaatggcgc
    17941 tggcgcttga gcctggtagt gagcaatatg ctccgctggc cagggcgatc atcgggggac
    18001 tggctgtgtc caccgttctt accgtatata ttgtgccttg cgtttacctc ctgattcatc
    18061 gtggggaaga gaaggaggta acggcatgaa gaacttgcca gtacttgttt ttttctttgc
    18121 ttcatgtgcc ttggcgcaat cttcattgcc atcggcaccg caggtttcgc tgaccttctc
    18181 acgttcgccc atgctgtcat ctgctcagtc tctgcccaac cagacagggg gtggacctgt
    18241 gcggctgaca cgggagcagg ccgagcagat cgcccttaaa aacaacccgc gcgtaagcgt
    18301 ggcccagttg cttgccatgg cgcaacatca ggtcgtccgg gagacccgtt cggcaatata
    18361 tccggatgtt tccggcaata ttacggccgt tcaggcagaa gaggccagcc gcatttcttc
    18421 aggcgccttg accgcgtccc gtctgttgca acatgcagga ctcggggtag agctctcaca
    18481 actgattacg gacttcggac gaactcacaa cctgatttct tcggcaaagc tgactgagaa
    18541 atcacaacag gccagtgcag aagcaacccg tgagcagatt gttctggcga ctgactttgc
    18601 cttctacaat gctctggaag cccaggccac actcaatgtt gctaatcaga ccgtcaaaga
    18661 gcggcaggca gtcgaagacc aggtgaacca gttgaccaaa acaaaactta agtctgatct
    18721 ggatctgagt tttgcgcagg tcaatttgtc gcaggccaaa ctcttacaac ttgaagcaca
    18781 ggacaactat gacaccgcca tagcgagtct gaccactgtt ctgggttcgg atacgcacca
    18841 gaaatatgag ttgatagacg acacgacgca acttccgtcc ccaccgccgg atgaaaactc
    18901 tcttgaggcc caggcgatgc agcaacgccc ggatttacag gcgattgatc tgaagcatca
    18961 ggccgaccag aaatttgcgc gtgcgcaatg gcagcagctt ttgcctacca tcagcgcact
    19021 gggggtggtc ggcaagacgc cggtaggctc cagtcagtat ttcatcggcg attggtacgg
    19081 agcggtaggc ggaaacctca atatccctgt atttaatggc tttcgctaca cagcacaggc
    19141 ccatgaggca tatctgcgcg cgaaagcgtc agcggaacag atccgggacc tgcgtgatca
    19201 gattgtccgt gacgtgcaga ccgcgtggtt acgcgccaac acggcctatc aaaaggtggt
    19261 tgtgcagcag gagctgttac gcgaggccaa tatgggactt gatcttgcgc aaacacgcta
    19321 tcaacttggg ctaagttcaa ttgtggaact gagtcaggca caattgcagc agacagaggc
    19381 tgcgattggc gatgccaacg ccaggtatca gtaccagttt gcaatctcca cgttgaatta
    19441 ccagattggc gtgcatccat gaccatcgtt cactctttct gtgtggtgcg acttaacaat
    19501 acgtagcctg aaacgccagc caatagggat gcggtcagga cagccgtctt ggccgcatca
    19561 agctgcgcgg gatcagcaaa tgcaagcgcg ctgataaaga gagacaccgt aaagccgatg
    19621 cccgcaatcg tgcctacact tactatctga gcccctttca cttttccggg aagctgcgcg
    19681 atcttcagct ttaccgcgat ccagcaggcc gcagtaatgc cgagcggctt gccaatgagc
    19741 aacccaagca gtactcccca tgcaagaggg ctgtgtagaa gcgatttcca tgtggtaaga
    19801 gagaacgtca ctcccgcatt cgacaacgca aacagaggaa gcacgagaaa agcaatccag
    19861 tcatgcagct tccgcgtaat gcgctctgcc ggagattcag tgttggcaat aaggtactcc
    19921 atggctccca actggcggtt ggcaagagaa ttgtcaccag acgcgcgcgc ctttttgaag
    19981 tcctcaagca cctcgctgca cacatcgccg aactggtcga gcgaaacact cgacgtcacc
    20041 ggcacgagaa atccgagaac aaccccggca atggtggcat ggacgccaga gcgcaggatt
    20101 gccagccaca ctccgaagcc cagcgcagca taaaaaatct gacgacggat accgatcctg
    20161 cggcaaagca aaatgatcgt gaccagcaga gcagccatca gcaggtagtt cagatgcagg
    20221 gtgtccgtat agaaaatggc gatgacggca atggcgccga tgtcgtctgc aatggcgagt
    20281 gaaagcagaa aaacctttaa ttcagcggga acgcctttga ccatcgccag aacgccgaga
    20341 gaaaaagcga tgtcggtggc catgggaact ccccagccgt gcgctcccgg tctgccgtag
    20401 ttcagcgctg cgtagatcat ggcgggaagg accattccgc ccagaccgcc gcagataggc
    20461 agagaggcgc ggcgcaggtc cttgagttca ccgtgcacga tttcctgttt gatttccatt
    20521 ccaacgagaa agaagaacag cgccataagg gcgtcgttga tccactcatg cacagggagc
    20581 tcaagaccgg aaagagtgag atgaatatgc cagatgtgat agtaagacgc gctccagggc
    20641 gagttggccc agataagagc aaccaccgcc gcagccagca aaaatgcgct gctcacgccc
    20701 tgcgtatgaa taaattgctg aatgggcaat tgcagggcgc gcgtaatcag cgattcgcgc
    20761 acctgcagct taagttctcc aagagtacgc gccgggatcg tcattgcaga cagtgtatgg
    20821 aatgggcgga catacgacaa tcaaaaatgc agtatcatgc ttctgatgct tgcccctctg
    20881 cgcttccttt ggaatgccac acgcggccat cggttcagtc cgtggcgcag cgaatatctg
    20941 aaatggcgca tcgagaccta ttctgggaag aaagctgaaa cgctgcagtc acgcgatgta
    21001 atcgcctttt tatggcagca aaagtggatt ctcttgcgct tcctgcgctg gacaggtgaa
    21061 atggacagtt tcagaaaaat ttcttctcgg gtcaacgaag agtgaccatg cgtaatgcaa
    21121 cgcgccgtga atttttgaaa ggcgcagcct gcctggggct ggcgggcctc atgccgcttt
    21181 ctgccgaaaa agatataggt gatccgacct cgttgcgaac acatgcagaa gcacgcggat
    21241 tgctggtagg gtgcgctgtt aatccagaaa aactacgcga tgaaccgctg tacgcgcaga
    21301 ccgtggccga acaggaaagc cttttggtag cggaaaatgc gatgaaatgg cctgcgcttc
    21361 atcctgctcc agacaaatat aatttcgcgc cggcagattc catccttgcc tttgccgaag
    21421 cgcataaaca gcagttacga ggccataacc tgtgctggca tgaggctttg cctgactggt
    21481 tccgttctgt ggttacaaag gaaaatgcta accatgtcct tgcagaacat atccgcacag
    21541 tcgcgggaca ttttgctgga aaattgcatt catgggatgt cgtgaacgaa gccattgatc
    21601 ccaaagacgg gcgtcctgat ggactgcgca aatccccctg gctggagctt gccgggccgt
    21661 attacatcga aacagcattc cgcgctgcac ggcaggccga tccaaaggcg cttctgacgt
    21721 ataacgatta cggtatcgaa ctggacaccc ccgatcagac cgcgaagcgc gagcaggtgc
    21781 tggctctggt ccagagactg aagaagcgca acgtccccat tgacgctgtt ggagtacaaa
    21841 gccatctcac tgcgggagat ccaacccctg gagcgggcct gcgcgatttt gtccgaaagc
    21901 tgcgcagtat ggacatgcag gtgttcatta ccgagttgga tgtttccgac cacaagatac
    21961 aggggaccat cgctgagcgc gatgcagcag tggcaaagct ctatggcgac tacctgcgaa
    22021 tgatgctggc cgaaccaaac gtaaaagcag tgctcacatg gggcatcacc gacaaataca
    22081 cctggctgaa ccatcagcac aagcgcagcg acggtcagcc acaacgccca ttgcctttcg
    22141 acgcggaata caagccagct ccggcattct atgccatgcg ggcggcggtg gactcacgtc
    22201 cttcctgaga gaacaaatac aatcattctt tgaggaaaat gcaatgttca atcctttaaa
    22261 aattcaggaa atgatggccc aggcaaaaca aatgcaggag caaatgcagg aaaaactggg
    22321 ccagaccatt gttgaggctt cgtccggagg cggcgcagtt acggtaaaaa tgaacggtca
    22381 aaagcaagtg ctgaaggtca cgattgatcc gagcgcagta gccagtctca gtggcaacgc
    22441 aaatgacgtt gaaatgctcg aagacctgat tgccgccgcc tttaacgaag ccggacgccg
    22501 cgccgacgag gccatcaaat cggctgcatc ttccatgctg ggtggactga acctgccgcc
    22561 cggactgctg tagtcctttt ctttctggac aatcatgtcg gcccagagca tcatgaacac
    22621 acagaggcgt atatggcgat ggcgcccata ttccggtgag cgaatatctt ttcatatgag
    22681 cgatcaccgg tgaaaaacag cagtcccgga ccataaaaac aaacgcattc acaaggaagg
    22741 aaagaacagg aatgttgacg cggcgcgtgt ttttaaagca ggccagtctt gcaggcgcag
    22801 cctggatgac cgccccttat atgtcccggg gccagcagac atcggtctcc aatgacagaa
    22861 tcaggcagtt ccgcgaaggc ggggccacga ctcccatcca tgtcacaaac ctgcgggaca
    22921 acctatatct gctgcagggt gttgggggaa acatggtggt gcagacaggc ccggacggta
    22981 cactgttgat agattccagc gcatccacgg ccgcgccaaa gattgaagac gctttgaaga
    23041 agcttgatgc gcatccattg aagtttctgg tgaatacgca ttggcattac gaccatacag
    23101 atggcaatgc cgctctgcac gggagcaccg gagtgtttat cattgcgcat gagaacacac
    23161 gcaggcgcct gtctgctcca caggaaattg catttctgaa aatgcgcttt gacgctaatc
    23221 ctccggcagc gttaccgcaa cagaccttca cggacaacca aaaaatgttc ctgaaccagg
    23281 atgaactgcg tctcgggcac atgcccgctg cgcatactga tactgatatc tatgtccact
    23341 ttgtgaacgg caatgtcatt catgcgggtg atatcttctt caacggactg tatccgttca
    23401 ttgacggcag cactggcggt actttccacg gaatgatgag ggcagcggaa acttgcctgg
    23461 cccttgcaga caacgagacg aagatcgttc cgggacatgg accgctgggc gacaaagcct
    23521 ctctgcagaa ataccatgac atgttgagcg gcctcgctga acgggtgggt aagctgaagg
    23581 catccggcaa gagcagggaa gaggtgattg ccgccaaacc tacagcggac tttgatgcag
    23641 aatggggcaa aggctttctc cttccagacc agttcgccgg gctggtttac gacacgctgt
    23701 aatttcttat tttcctgatt gactctcccg taactggaga ccttacaact gactcgtgtt
    23761 caagattggt gagttttccg cgctgacgcg catttcggtc aagacgctgc gcttctacga
    23821 tgaagtgggg ctgctcaagc ctgcccacgt tgacacctgg accgggtacc gctactactc
    23881 agcccagcaa ttgccgcgtc tgcaccggat attggccctg aaagacctgg gcttttctct
    23941 ggagcagatt gtcaaggcgc tcgacagtaa catcagtccg caggagcttc gcggcatggt
    24001 gatgctgcgt cgggccgagc aggagggccg cgtgcgtgaa gaacaggaac ggctcaaccg
    24061 gttggagaca ctcattaaat ttatagaaca ggagtcagtt atgagtgcag atgttgtgat
    24121 gaaagaagtg cctccgcagt ggatcgtctc cgtacgcgag gtcatcccga actatccatc
    24181 cgtaggaatg ctctatggaa aggtcatggg ccagattggt ggtggctgca atctgggcat
    24241 gcccgtggcc atttggcatg acctggaaca taaagagaaa gatgtagacg cagaggccgg
    24301 tttcctgctg aaagagcctc tcgccgccca cggaacagcc cagatgtatc agctccaacc
    24361 cgccttagtc gccagctaca tgcaccatgg cgcctacaac cgtcttgacc aggcatacca
    24421 gaacctgctc cgttgggtgg aagagaacca ctacaaactg accggacctt tccgtgaact
    24481 ctaccattac tgcgcgcagc ctgtgcggca ggacgacgag tcctatgtga ccgaaattca
    24541 gattccggtg gagaaaagag aaggttaaat gggtctgtgc atgaatcgtg gcaaagcgaa
    24601 cggaatcctg ttaaaacagc ggtgaggtat cgttttgtca cgatttgcag aacccatggc
    24661 gcggctcatt gacgaactgc gcaaacttcc cggaatcgga gcaaagaacg cgcagagact
    24721 ggcatttcat atactgcgtt cgagccatga agatgcggaa gctctcgcag acgccatcca
    24781 cagtctgaaa gaaaaactcc agctttgctc tgtttgcaac aacattacag atatggatcc
    24841 gtgtgtctat tgttccagcc cgacacgtaa ccagcggctg gtctgtgt
//
LOCUS       DASRBV010000098         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_562257, whole genome shotgun sequence.
ACCESSION   DASRBV010000098 DASRBV010000000
VERSION     DASRBV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562257"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1321)
                     /locus_tag="VFA02_03980"
     CDS             complement(227..1321)
                     /locus_tag="VFA02_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZU09036.1"
                     /translation="MLHGKLRKKRFVYRQHLAPQIVEWQARASYRMKVFIGTNGFKMG
                     GRLKSCTRSFLANTRKLTVVVYNELWRTRAFTAAAGLAYYFLLSLVPLIIVFASLLRF
                     LPVNNIAFQLLGLIAALVPPQSMNLVLSAVAAILEPGHGKVLSFGVIGYLWAATGGFS
                     ALIEALNIAYDVEVSRPWWRDRLQAFLMTFTVGALGLFALLAILAGPHFGHLLMLIFP
                     LPQSFGMLWPVLRWTIIVVTFIAGIELLYYLGPHARHSYWSTLPGALLAVGIWFAGSF
                     GLSFYLSNMSSYNKTYGPLGAVIALMLWLYISGLAVLIGAELNAEWNKAKQTRKKQLE
                     EAPCAAPQEQRTTMQRQRSAQAWRRPGKQI"
     gene            1313..2053
                     /locus_tag="VFA02_03985"
     CDS             1313..2053
                     /locus_tag="VFA02_03985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU09037.1"
                     /translation="MKHLLAFVLLLLTTPVFASTPQQVTFPSGDGHASGLLYLPSGSG
                     RHPAIIVIHEWWGLNDWVKEQASNLASEGYVALAVDLYRGQVATDPEMAHELSRGLPQ
                     DRGVRDLVSAFAFLAKRKDVDPKRIGAVGWCMGGGYAAQFAVAEPDLRAVAINYGPLP
                     TDKTALEKIRAAILGNFGGQDRGIPPSSVNAFVDTMKSLGKQVDAKIYPDAGHAFENP
                     NNTQGYKPEDAKDAQERMRRFFAQTLKP"
     gene            2210..2557
                     /locus_tag="VFA02_03990"
     CDS             2210..2557
                     /locus_tag="VFA02_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU09038.1"
                     /translation="MKIGTTIRGYRLQKGLSQGDIEKRTGLLRCYLSRVENGHTVPSL
                     DTLSKIANALDLPLSQLFAEDALGQELNTQKLTDDELRFLTQIRRYSSNLNDSDRKLL
                     LAMVKKFAATAVR"
     gene            complement(2612..3511)
                     /locus_tag="VFA02_03995"
     CDS             complement(2612..3511)
                     /locus_tag="VFA02_03995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZU09039.1"
                     /translation="MGAWQNSGEHQDERPYSTHTGPEILPPEGREEEQGWYYQSPEPE
                     YTPPVEPKRRSRWAYAPATYVLVGINCFVFLVMLLTGGSALEPTLRSPLFSAFALNGG
                     VVLYYGEWWRLFTATFTHVGWIHLLSNMWCLWNLGLLGEPLLGPFGMLAAYVLTGLGG
                     NLLSTAVHPGLPGTADGIIGAGASGAVFGLAGVLIILLKSPLLPLPRMEVMRLRKSVI
                     WFAVLNFVIGAGTWVARTRVQIDNMAHLGGFLSGLALALPLVPRIGAPRETFLRRRRF
                     AVFGMGFLLLLIAFGVYSFYMGR"
     gene            3908..>4465
                     /gene="purH"
                     /locus_tag="VFA02_04000"
     CDS             3908..>4465
                     /gene="purH"
                     /locus_tag="VFA02_04000"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567520.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZU09040.1"
                     /translation="MSSYKQVRRALLSVTDKTGLVDFARRLAKHGVELVSTGGTAKAL
                     REAGLQVRDISELTGFPEMLDGRVKTLHPKVHGGILHIRENAQHVAAVKEHGIEPIDM
                     VVVNLYAFEKTASRPGAKFAEIIENIDIGGPSMVRSAAKNFEDVAIVTSPADYAALAE
                     ELDANQGALGRETRWRLAKKAFATTA"
BASE COUNT         1096 a         1278 c         1160 g          931 t
ORIGIN      
        1 ctctcccgag tggtatggag cgcggaaaga tgcggctgat agtctttgcg caaaaaggcg
       61 aaaatggtga agtcctggga gcagccgaac acgacattta acgtacaact gagcaaacag
      121 gcaaaggcaa attcaatggc ctgtctggac tatctgtcgc tgcgctggcg accgggcggg
      181 gtaattgggc tggttgtctc ggaaatgttg gtacagtatc cgggattcag atctgctttc
      241 ccggccttcg ccatgcttgc gctgatcgct gcctttgcat tgtcgttctt tgttcctgag
      301 gcgcggcgca cggcgcttcc tcaagctgtt tcttcctggt ctgttttgcc ttgttccatt
      361 ccgcattcag ctctgcgcca atcaatacag ccagtccgct gatataaagc cacaacatca
      421 acgcaatcac ggccccgagt gggccgtagg tcttgttgta gctcgacatg ttgctgaggt
      481 aaaagctcag tccaaatgac ccggcaaacc atatgcctac cgccagaagc gccccaggca
      541 gcgtggacca gtaactgtgc cttgcgtggg gcccaagata gtagagcagc tcaattcctg
      601 caataaaagt cactacaatg atcgtccagc gcagaacagg ccacagcatt ccaaaacttt
      661 gcggcagggg aaatatgagc atcagcaaat gaccgaagtg tgggccggcc agaattgcca
      721 gcaaggcgaa cagccccagt gcaccaacgg taaacgtcat caaaaaagcc tgcaggcggt
      781 cgcgccacca gggacggctg acttccacat cgtaggcaat attcaacgct tcaatcagcg
      841 ctgaaaaacc tcctgttgcc gcccaaagat atccaataac gccaaaagag agcaccttcc
      901 cgtgccctgg ctccaggatc gctgccaccg cagagagcac caggttcatg gattgtggtg
      961 gaactaaagc ggcaatcaat cccagcaact gaaatgcgat gttgttcaca ggcagaaacc
     1021 gcagcagcga agcaaacaca atgatcagcg gaacaagcga tagcaaaaaa taataggcca
     1081 atccggcagc ggcggtaaaa gcacgtgtgc gccacaactc gttatagaca accacggtca
     1141 actttcgagt gtttgccaga aagcttcgtg tacagctctt cagtcgtccg cccatcttga
     1201 acccatttgt tccgatgaac actttcatcc ggtaggatgc gcgggcttgc cattcgacga
     1261 tttgcggtgc aagatgctgg cgatacacaa acctcttttt gcggagcttt ccatgaagca
     1321 tctccttgca tttgttttgc ttctcctcac cactcccgta tttgcctcca ccccacagca
     1381 agtcaccttt cccagcggcg acggccatgc ctccgggctt ctttacctgc cttcaggatc
     1441 tgggcggcat cctgccatca tcgtgattca cgaatggtgg ggactcaacg actgggtcaa
     1501 ggaacaggcc agcaatcttg ccagtgaagg atacgtcgcg cttgccgtgg acttatatcg
     1561 cggacaggtg gccacagatc cggagatggc gcatgagctt tcccgcggac tgccgcagga
     1621 tcgcggcgtg cgcgacctgg tgtcggcatt tgcttttctg gcaaagcgca aagatgtaga
     1681 cccgaagcgc attggtgccg tgggctggtg catgggcgga ggctatgccg cgcagttcgc
     1741 cgttgccgag ccggatctgc gcgctgtcgc catcaactat ggacctttac caacggacaa
     1801 aacggccctg gagaaaatcc gcgcagccat cctgggaaac ttcggcgggc aggaccgcgg
     1861 aatcccaccc tcttctgtaa acgcctttgt tgacaccatg aaaagcctcg gcaagcaggt
     1921 agacgcaaag atttatcccg acgccggaca tgcctttgag aacccgaata atacccaggg
     1981 atataaacct gaagatgcaa aggacgcaca ggagcgcatg cgccgtttct ttgctcagac
     2041 actcaaacca tgagctttaa gccgcaccat ctttggttcc tggaaagtaa tagttacgaa
     2101 agtccgaaaa tttctcttgc ttctggctct gtggcgtgtt aactatgcag ccaacacaat
     2161 aaaaaaaacc gctcttccag agatggaaaa gcgggaaatg ggctaagcca tgaaaattgg
     2221 aactaccatt cggggttatc ggttgcaaaa agggttgtcg cagggtgaca ttgaaaagcg
     2281 cactggcctg ctgcgctgct acctgtcaag ggttgaaaac gggcataccg ttccttccct
     2341 tgacacgctc tccaagatcg caaacgcgtt ggatttaccg ctctcacaac ttttcgccga
     2401 agacgcgctc ggacaggaac tgaatacgca gaaactgacc gatgacgagc ttcgcttcct
     2461 gacgcagata aggcgctatt cttccaacct gaacgacagc gaccgcaagt tgctgctcgc
     2521 catggtgaaa aagttcgcgg ccacggccgt acggtaaacg cattttgaaa aagcagagtg
     2581 cctacatcgc aaaagatgta ggcacgctgt tttatctgcc catatagaac gaatacacgc
     2641 caaacgcaat cagcagcaga agaaaaccca tgccgaagac cgcgaaacgg cgacggcgca
     2701 gaaatgtctc acgcggcgcg ccgatgcggg ggactaatgg caaggccagg gcgagtccgc
     2761 tcaggaaacc tcccagatgc gccatattat ctatctggac gcgggtgcgg gcgacccatg
     2821 tccccgctcc aatcacgaag ttcaacaccg caaaccagat gacgctctta cgcagccgca
     2881 tcacttccat gcggggcagc ggcagcagag gcgatttcag cagaataatc agcactccgg
     2941 caagtccgaa gacagcccca gatgcccccg ccccgataat cccatccgca gtgcccggaa
     3001 gtccgggatg gactgctgtg ctcagcaggt ttccgcccag tccggtcagg acataagcag
     3061 ccagcattcc aaatggcccc agcaacggtt cacccagaag gcccaggttc cacagacacc
     3121 acatattact cagcaggtgg atccatccca catgagtaaa cgtcgccgta aagagccgcc
     3181 accattctcc gtaatacagg accacgcccc cattcagggc aaaagctgaa aacaaggggc
     3241 ttcgcaaggt cggttccagg gcggaaccgc ctgtaagcaa catcacgagg aagacaaagc
     3301 aattgatgcc caccagcaca tatgtcgccg gagcataggc ccagcgcgaa cggcgcttag
     3361 gctcaacagg tggcgtgtac tctggttccg gagactggta ataccacccc tgctcctctt
     3421 cccttccctc cggtggaagg atttccggcc cagtatgcgt ggaatatggg cgctcatcct
     3481 gatgttctcc gctgttctgc catgcgccca taaaatcagt ctcatttaac cttaccccac
     3541 ttctggccct tctgcagtcc agcaatgttt agatccacgg tccatctctt tataaacaga
     3601 aagcagtgat gttcttcgtg agagcgtgcg caatggtttt tctgtatcgc gtgatgagat
     3661 gaatgatcgt tttcctgctc tggagtcaaa gttggaccgc ttagatactg aggtccgcaa
     3721 agatgataaa aaccgcatcg ccagactgga agacgcgtat ttatgtgttc cgcgcgttag
     3781 tgttcccgtg gttctgccac ctgcccaaac ttcccggagc tcacccgata tgacaatttc
     3841 ggcctcgcct gcttacaatt ttcttttcac ctcttctttt cttctcacct caaaggagca
     3901 ttaggttttg agcagctaca aacaggtgcg gcgcgcgcta ctgagtgtca cagacaaaac
     3961 cgggctggtg gatttcgccc gccggttggc aaagcatggt gtggaactgg tttccaccgg
     4021 aggcactgca aaggccctgc gcgaagccgg gttgcaggtg cgcgatattt ccgagctgac
     4081 cggattcccc gaaatgctgg atggccgcgt caagaccctg cacccgaagg tgcatggagg
     4141 cattctccat atccgtgaga acgcccagca cgtggctgcg gtaaaagagc atggcattga
     4201 gccgattgac atggtagtgg tcaatctcta tgcctttgag aagaccgcct cccgccctgg
     4261 cgcaaaattt gccgaaatca ttgaaaacat tgatatcggc ggtccgtcca tggtccgttc
     4321 agccgccaag aactttgagg acgtggccat cgtaacatcc cctgccgatt acgctgcgct
     4381 cgctgaagaa ctcgacgcca atcagggagc tttgggacga gaaacccgct ggcgtctggc
     4441 aaagaaagcc ttcgcgacaa cagcc
//
LOCUS       DASRBV010000099         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_5064,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000099 DASRBV010000000
VERSION     DASRBV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFA02_04005"
     CDS             complement(<1..420)
                     /locus_tag="VFA02_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZU09041.1"
                     /translation="MAQDRILFFDTTLRDGEQSPGCSMTTQEKLTMAHALDDLGVDIL
                     EAGFAIASEGDFQAIEAVAKEIRRPCIASLSRARREDIEKAAAALEHAERARIHIFLA
                     SSDIHLKYKLKISREEALEQAHKAVALARIYVDEVEFS"
     gene            653..1594
                     /locus_tag="VFA02_04010"
     CDS             653..1594
                     /locus_tag="VFA02_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU09042.1"
                     /translation="MELSQLETFLTVVAEKSFSRAAARLHRTQPAVSQVIRKLEEELG
                     EALFERTSRDGTLTAAGEVLREYAEKLLRLRNEASSALAEVKALERGRITLAANEYTC
                     LYLLPVLHEFRRHCPQISILVQRSLARRIPDEVLGRTVEMGIVSYRPEEEQLGAIAVY
                     SDDLVFIVPPRHSLAREKNARIRDLGAENFIAHNVPSPMRRRVEESFAKHKTPLNIGV
                     ELPSLEAIKRFVASGNGVAFAPALTVQAEASRGELVIVDVPELAYARKLWLIYRKFGT
                     PSYAALAFLRAVRALAREKGPPYLYDEKGNTTSKKQQ"
     gene            1725..>2212
                     /locus_tag="VFA02_04015"
     CDS             1725..>2212
                     /locus_tag="VFA02_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZU09043.1"
                     /translation="MKKILLSFVLLPVLAVTGFAQESRQDVSASWSGLIPPHTVGNAV
                     QQDGTIGNYGVLASYRYMLTPRSALELNYQYNQDIQHFVGNFFNWRVHTRFQEISGAY
                     VYNFTFRNFNPFLEAGIGGYIFTPIKDPKTTNLDVKQNTNVGALYGGGIAYELSPSWD
                     IRV"
BASE COUNT          486 a          652 c          579 g          495 t
ORIGIN      
        1 cgaaaactcc acttcgtcca catagatccg tgccagggca acggccttgt gcgcttgctc
       61 caatgcctct tcgcggctga tcttcaattt gtatttgaga tgaatatctg agctggccag
      121 aaagatatgg atgcgcgccc gctctgcgtg ctccagcgct gccgcggcct tctcgatgtc
      181 ctcgcgccgc gcacgcgaca aggaagcaat gcacggccgg cgaatctcct ttgctacggc
      241 ctcgatcgcc tgaaaatcgc cttcggatgc aatggcaaat cctgcctcca gaatgtccac
      301 acccagatca tccagggcat gggccatggt cagcttttcc tgcgtggtca tgctgcatcc
      361 gggcgattgc tcaccgtcgc gcagggtggt gtcgaagaaa aggatgcggt cctgtgccat
      421 ttcggtgttc cttggtacct gactggatag aaatcggcta aaaagcggtt cgaaaaaaaa
      481 aagactactc ctcggctgac agctttatcg ctggcagttt ttagccgact ttccggtccc
      541 gtgtaccaag tgtcctactc ggcaacatat aagtcaaatt ttaattttct ttattttcca
      601 taaattttaa ttataattct ctattgaggt cacatcaaca cgtaaattgc ttatggaatt
      661 aagccaactt gaaaccttcc tcactgtcgt tgcggaaaag agcttttccc gcgcggccgc
      721 tcgtctgcac cgcacgcagc cggccgtcag ccaggtcatc cgcaagctgg aagaagagct
      781 gggagaagcg ctttttgaac gtacttcgcg cgacgggact ctcacggctg ctggtgaagt
      841 tttgcgcgaa tacgcagaaa aactgctgcg cctgcgcaac gaggcatcga gcgcgttggc
      901 cgaggtaaag gccctcgagc gcggacgcat cacgctcgcc gccaacgaat acacctgcct
      961 ctatctattg cccgtgctcc acgaattccg caggcactgt ccgcagattt ccattctcgt
     1021 ccagcggtcg ctcgccaggc gtatccccga cgaggtgctg ggccgcaccg tcgagatggg
     1081 catcgtctcg taccgcccgg aagaggaaca actgggcgcc atcgccgtgt attccgatga
     1141 tctggtcttc atcgtgcctc cgcgccactc gctcgcccgt gaaaaaaatg cgcgcatccg
     1201 cgacctgggc gcggaaaact tcatcgccca caacgtccca tcgcccatgc gccgccgcgt
     1261 ggaagagtcc tttgcaaagc acaaaacgcc actgaacatt ggtgtcgagt tgcccagtct
     1321 ggaggccatc aagcgcttcg tcgccagtgg aaatggcgtt gcctttgctc ccgcattgac
     1381 cgtgcaggcc gaagccagtc gtggcgagct tgtcattgta gatgtgccgg agctggctta
     1441 tgcacggaag ctctggctga tttaccgcaa attcggcacg ccttcctatg cggcgctggc
     1501 ctttctgcgc gcagtgcggg cactcgcccg cgaaaaaggc cctccgtatc tttatgacga
     1561 gaaaggcaac accacgtcta agaaacaaca gtagctgtgc aaaaaccgtc tggcaggtac
     1621 aattgcggag tgggcctttc cctggcccgc ttaactcaac aattgacagg cccaaggggc
     1681 attgcgcctg ccgggctttt gtgcaggcga gaggttcata acggatgaag aagattttgt
     1741 tgtctttcgt gttgctgccg gtgctggcgg taactgggtt tgcgcaggaa agccgtcagg
     1801 acgtaagtgc aagctggtct ggccttattc ctccccatac agtcggcaat gcagtgcagc
     1861 aggacggcac catcggtaac tacggagtat tggccagcta tcgttacatg ctcacgccgc
     1921 gcagcgcgct cgaattgaat taccaataca accaggacat ccagcacttt gttggaaact
     1981 tctttaactg gcgcgtccac acccgttttc aggaaatctc cggagcatac gtctacaact
     2041 tcaccttccg gaacttcaat ccgtttctcg aagccggaat cggcggatac atcttcaccc
     2101 caatcaagga cccaaagacc accaaccttg acgtcaagca gaacaccaac gttggcgcgc
     2161 tatatggcgg cggcattgcc tatgaattaa gcccaagctg ggacatccgc gt
//
LOCUS       DASRBV010000100         8247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_156469, whole genome shotgun sequence.
ACCESSION   DASRBV010000100 DASRBV010000000
VERSION     DASRBV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156469"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFA02_04020"
     CDS             <1..340
                     /locus_tag="VFA02_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09044.1"
                     /translation="QKKFFFTDTDDNSRMEFLKLKTYWLSGVLGNGQPVWIADPRDAQ
                     YLNTSAEELQRMAADLAKQGWLALKDDYAAATPALTALSGEYHAKLQTALDITKPTFN
                     EEMRHGHTNM"
     gene            478..1575
                     /gene="ychF"
                     /locus_tag="VFA02_04025"
     CDS             478..1575
                     /gene="ychF"
                     /locus_tag="VFA02_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZU09045.1"
                     /translation="MPLNCGIVGLPNVGKSTIFNALTSAKAQAANYPFCTIDPNVGVV
                     TVPDPRLDKIAAIFQPKRVVPTTMEFVDIAGLVAGASKGEGLGNQFLGHIRSTDAVAH
                     VVRCFDDPNVIHVAGKVSPLDDMDVINTELLLADLETIEKRYTKVEKAAKSSTDAKVK
                     AEASALAKLREAVQAGKPARVVELTEEEKLAARELQLITAKPVLYVANVDEAGLHSGN
                     EWVAAVEKRAAEEGSQVVRICGAMEAEIAQLDLPERAEFLQEMGLEEPGLNRLIRSAY
                     KLLDLITYFTAGPQEARAWTIRRGTKAPQAAGVIHSDFERGFIRAEAYYCDDLFTLGS
                     EQAVKEKGLYRSEGKEYVVKDGDILFFKFNV"
     gene            complement(1565..1840)
                     /locus_tag="VFA02_04030"
     CDS             complement(1565..1840)
                     /locus_tag="VFA02_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09046.1"
                     /translation="MLPIVHDAAQKQVLLAVSPVLKPRSLQGQFRLVALDKDHTLLEG
                     TTWYQNYFWPQMYWREWSDTIVHRIHMRVLEHVKAQAERQAIQKVTH"
     gene            2231..4423
                     /locus_tag="VFA02_04035"
     CDS             2231..4423
                     /locus_tag="VFA02_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZU09047.1"
                     /translation="MAASQDFQPYVPATESRPEFTVRAVVLGCIFALLFGAVTVYVGL
                     RAGLTVAASIPIAVLSISVLRALGKASILENNIVQTTGNAGQSIAAGVIFTLPALIFL
                     GFDLEYIRIFMLALLGGWLGVLFMIPLRRQLIVEEHGNLTYPEGTACADVLIAGERGG
                     SFASRVFFGLGLGALYTLFQNENLLGAWPGTPNYEPKWLPGAAVRAEATPEYLGVGYI
                     IGPRIAGTLFAGGIISWLVIMPAIHFFGAHYPQAIYPGTVPIAQMSPSDLWRTYIRPM
                     GAGAVAASGLITLLRTMPTIIGALRAGVKMMGKNAAADRSSRTERDIPMSIVLAGSAL
                     LVLAIFLFLTFKPVPGAYTGWLANLAAALLILVFGFLFVTVSSRIVGLIGSSANPISG
                     MTIATIMATAAIFLVKGWTAPAFGALAIMIGGVVCIAAANAGDTSQDLKTGYIVGATP
                     WKQQVAVMVGVIVSVFSIGITLNAMNKGLETFMLLKQPVPFNITEPHDGVQVQQQHFS
                     RQRVLLTKPDNSSSFVDGGQYVLLNALGSRTLADGKYLYNPKTGMIEVQWVQGIGSEK
                     AAAPQGRLMATVINGILSRKLPWGLVFLGVFLVLAIEVLGVRSLSFAVGAYLSIGTTL
                     AMFCGGIVRWMADRAVEKAGGSASDMESEVSPGSLYASGLIAAGGVMGLLGVARKLYE
                     TATGNELHVWFAEGSVVDRLLHHQGVSIAAFVLLAFSLYYFARKPLKE"
     gene            complement(4435..5583)
                     /gene="purK"
                     /locus_tag="VFA02_04040"
     CDS             complement(4435..5583)
                     /gene="purK"
                     /locus_tag="VFA02_04040"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0034028 - 5-(carboxyamino)imidazole
                     ribonucleotide synthase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZU09048.1"
                     /translation="MSALLPGSTIGILGGGQLGRMTAMAARSLGYRVQVMDPDPSCPA
                     RFVVDACFEGGWDDSRAAADLARGSDVVTLEIEQVSIASLEAAAKFAPVRPGIAMMQI
                     IQDRILQKNWLAENGFPVGEYRAIHSEADLQSAASALGGKLFVKSARGGYDGRSQAKI
                     GFGENASSGIAEAAEAWKSLGERQAIAEKALDLDKEISVMVARAPNGETKSFPTATNH
                     HERQILAWSVIPSVIPSSLEEKAQKLARDIADKMHLEGVLAVEMFLTRDGRLLINELA
                     PRPHNSYHASERACVTSQFEQIVRAVCDLPLGDTSVVAPAAIVNLLGDLWLNHEPRFD
                     QALRVPGVRLHLYEKHQPRKGRKMGHLSAVGKTPEEAVQRVLEAEALL"
     gene            complement(5580..6071)
                     /gene="purE"
                     /locus_tag="VFA02_04045"
     CDS             complement(5580..6071)
                     /gene="purE"
                     /locus_tag="VFA02_04045"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZU09049.1"
                     /translation="MEHAPLVGVVMGSKSDYEILKAAVDLLKELSIPYEVRVVSAHRT
                     PDWLFQYAEQAEERGLRVIIAGAGGAAHLPGMLAAKTLVPVLGVPVPATMLNGIDSLL
                     SIVQMPKGVPVGTLAIGKPGAANAALFAAEILATTDTELRHRLRAWREARKREVLAED
                     LPQ"
     gene            complement(6176..6457)
                     /locus_tag="VFA02_04050"
     CDS             complement(6176..6457)
                     /locus_tag="VFA02_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09050.1"
                     /translation="MANILLGVEHGIEVAAEDVLKFLTGVKDLTSKVDPDVIAGLGVL
                     LGTVAKAVEDVSAAAANPLNLTLDAATIQDLKAVWPALVKFAASIGIKL"
     gene            complement(6458..6667)
                     /locus_tag="VFA02_04055"
     CDS             complement(6458..6667)
                     /locus_tag="VFA02_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09051.1"
                     /translation="MNISTNNPQFFWIGVGVLWIFSAAVSAMDAPRADDSRFYRWLYR
                     FTHLLAANLDRAFGATINQQTTEKQ"
     gene            complement(6741..>8247)
                     /locus_tag="VFA02_04060"
     CDS             complement(6741..>8247)
                     /locus_tag="VFA02_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09052.1"
                     /translation="LWQVQDPGQHVSLTANGLTCSGGNGYDGQTFVSAISSFELGGSL
                     MVEATGLQFTAASTGILSGLYSGSVSAQNCIAGFQVTQNNGSNSVAAFVSGTVTGNAF
                     TPVSGHVYTLRTRIYAKEMQRVLQSYYSVDDTVVKLWGGTGIPCNIHCMLEIQDTTNG
                     VAQSPVILYDGVITNAPAAGVYAPLNSASLFCSIRTISITQQGPEWVISIPPGGSAVT
                     RRIGTTAQGADCRIERTGKLRFYATSVPQQGELIFVSYRTRRRAVARLANGAATSPDA
                     SSQVPATSRWMGTVTHPKPLSSVDCENAARAILDLASSRGAAWKGTYTGWNFETQGDV
                     WPGDLLAISATSSGMAANVVVRKVQIEMQNTSPQLNKYIIYFANDWAEPLAIKTSSTI
                     PADTWLPQQPQTTAPLDNLREMSVTSVSSSAIQISTNITPPSGGGFEVRRRDWSFGPG
                     TDSDLVLRSPVSNFTVPREAVMEQYYVRMYDSSTPPNYSRFSSAVFVNVPL"
BASE COUNT         1683 a         2298 c         2483 g         1783 t
ORIGIN      
        1 gcagaagaag ttcttcttca cggatacgga cgacaactcg cgcatggaat ttctgaagct
       61 caagacctac tggctctccg gcgtgctcgg caacggacag cccgtctgga tcgccgaccc
      121 gcgcgacgcg cagtatctga atacctccgc agaagaattg cagcgcatgg ccgcagacct
      181 ggcaaagcag ggctggctcg cgctcaaaga cgactatgct gcggcaacgc ccgcgctgac
      241 tgcgcttagc ggcgagtacc acgcaaagct ccagactgcg ctcgacatca ccaaaccaac
      301 atttaacgaa gagatgcgcc acggccacac caatatgtaa cggcccggtt gccgtcgtgg
      361 gcgaaacgac cgaagcgccg tcatcctgag cgaagtgtgg cggagtcgaa agccgggtgc
      421 cccgcatcct gaagggatgt gggctgttta caaatacttg tttaaaggaa caaaaccttg
      481 cctctgaatt gtgggatcgt tggcctgccc aacgtaggga aaagcaccat cttcaatgcg
      541 ctgacctcgg ccaaagcgca ggcggccaac tatcctttct gcaccattga tcccaacgtc
      601 ggtgtcgtga ccgttcccga cccgcgcctg gacaaaatcg ccgccatctt tcaacccaaa
      661 cgcgttgtgc ccaccaccat ggagtttgtg gacatcgccg gactcgtcgc cggggccagc
      721 aagggcgagg gactgggcaa ccagttcctc ggccacatcc gctcgaccga tgcggtggcc
      781 cacgtcgtcc gctgcttcga tgatcccaat gtgattcacg tcgccgggaa agtcagcccg
      841 ctcgatgaca tggacgtcat caacaccgaa ctgttgctcg ccgatcttga aaccatcgag
      901 aagcgttaca caaaagtaga aaaggcagcc aagtcatcca ccgatgccaa agtgaaggcc
      961 gaggcctccg cgctggccaa actgcgagag gccgtgcagg ccggtaaacc ggcgcgcgtg
     1021 gtcgagctga ccgaggaaga aaaactcgca gcgcgtgaac tccagctcat cacggcaaaa
     1081 cctgttctct acgtcgccaa tgtggacgag gccggcctgc actccggcaa cgaatgggtc
     1141 gcggcggtcg agaagcgcgc agcagaagag ggcagccagg tcgtccgcat ctgcggcgct
     1201 atggaggccg aaatcgcaca gctcgatctg cctgagcgcg ctgaattttt gcaggaaatg
     1261 ggcctcgaag agccaggact caaccgcctt atccgctcgg cctacaagct gctggatctg
     1321 attacctact tcaccgccgg accgcaggag gcccgcgcat ggaccatccg ccgcggcacc
     1381 aaagcgccac aggctgccgg agtcatccac tctgattttg agcggggatt catccgcgcc
     1441 gaggcctact actgcgacga cctcttcacg ctcggcagcg aacaggccgt aaaagaaaaa
     1501 ggcctctacc gctccgaagg caaagaatat gttgtaaaag acggcgacat tctcttcttc
     1561 aagttcaatg tgtaactttc tggatagctt gccgctcggc ctgcgctttc acgtgctcca
     1621 ggacccgcat gtgaatgcgg tgcacgatcg tatctgacca ctctcgccaa tacatctgcg
     1681 gccagaaata gttctgatac cacgttgtcc cttccagcag cgtgtggtct ttgtccaacg
     1741 caaccagccg gaattgtcct tgcagagagc gcggtttgag tactggactg acggcaagca
     1801 gcacctgctt ctgggcggca tcatgaacga tgggaagcat acatcgtgat tgcagcaggc
     1861 ggcagcaagt tcaggcacat ggacgacagc tattacgtgg cactcgtcct gcggcatttc
     1921 tgagcagacc gccgttgtgc ggtggagacg gcgtttcagg ccatggagca tctgaacaga
     1981 gcacgcaggc cacggccaac gggaccgaaa ggaaagcaac acaagctggt cagagagaaa
     2041 tggcgcaaag cgcatgtgcc tggacgggcc acggcatttt cacagattct gcacactcca
     2101 tttgcactgt gcagtgtcgc cgactccctg ttggtcgcgg cggctgtagt tgcgtggcat
     2161 tgtccagagt atctgtgaaa atgctacagt ccctgataca ctcatcagtt tcctggaggt
     2221 ttttcaaaga ttggctgctt cgcaggattt tcaaccttat gttccggcga cggagtcgcg
     2281 gcctgagttt acggtccgcg cggtcgtgct gggatgcatc tttgctctgc tttttggtgc
     2341 ggtcacggtg tatgtcgggc tgcgggccgg acttacggtt gcggcctcga ttccgattgc
     2401 tgtgctttcc atcagcgtgt tgcgggcgct gggcaaggcg tccattttag agaacaacat
     2461 cgttcagacg acgggaaatg ccggccagtc cattgccgca ggcgtgatct ttacgctgcc
     2521 cgcgctcatc tttctggggt ttgatctcga atacatccgc atctttatgc tggcgctgct
     2581 gggcggatgg ctcggcgtgc tcttcatgat tccactgcgg cggcagttga ttgtggagga
     2641 gcatgggaac ctcacgtatc ccgaaggcac ggcctgcgcc gatgtgctga ttgcaggcga
     2701 acgcggcgga tcgtttgcca gccgcgtctt ctttggactg gggctgggcg cgctctacac
     2761 gcttttccag aatgaaaatc tgctgggtgc gtggccggga actccaaatt atgaaccgaa
     2821 gtggctgccg ggagcggcag tgcgcgccga agcgacaccg gagtatctgg gtgtgggcta
     2881 cattatcggg ccgcgcattg cgggcacgct ctttgccgga ggcatcatca gctggctggt
     2941 gattatgccg gcgatccact tttttggtgc gcattatccg caggcgattt atccgggaac
     3001 ggtgccgatt gcgcagatgt cacccagcga tttgtggcgc acatacattc gcccgatggg
     3061 cgcaggcgca gttgccgctt ccggcctgat tacactgcta cgcaccatgc cgaccatcat
     3121 tggcgcgctg cgcgcgggcg taaagatgat gggcaaaaac gctgccgcgg accggtcttc
     3181 gcgcacggaa cgcgacatcc ccatgagcat cgttcttgcg ggcagcgcgc tgctggtgct
     3241 ggcgattttt ttgttcctga cgttcaagcc cgttcccggc gcgtataccg gatggctcgc
     3301 gaacctggcg gcggcgttgc tgattctggt ctttggattt ttgttcgtga cagtcagcag
     3361 ccgcattgtg gggctgattg gcagttcggc caaccccatc agcggtatga cgattgcaac
     3421 catcatggcg acggcggcga tcttcttagt caaaggctgg actgcacccg catttggcgc
     3481 gctcgccatc atgattggcg gagtggtctg cattgctgcg gcgaatgccg gagacacttc
     3541 gcaggacctg aagacgggtt acattgtggg cgcgactccg tggaagcagc aggtggccgt
     3601 gatggtgggc gtgattgttt cagtcttttc gattggcatt acactgaacg cgatgaacaa
     3661 gggcctggag acattcatgc tgctgaagca gccggtcccg ttcaatatca cggagcccca
     3721 cgatggcgta caggtgcaac aacagcactt ttcgcgtcag cgcgtccttc ttacgaaacc
     3781 ggacaactca tcttcctttg tggatggcgg ccagtatgtg ctgctgaatg cgctggggtc
     3841 gcgaacgctg gcagacggca aatatctcta caacccgaag acaggaatga ttgaagtgca
     3901 gtgggtgcag ggcatcggca gcgaaaaggc cgccgcgccg cagggccgtt tgatggcgac
     3961 ggtcatcaac ggaattctct cgcggaagct gccgtgggga ctggtgtttc tgggcgtgtt
     4021 tctggtactg gcgatcgagg tgctgggcgt gcgctcgctc tcgtttgcgg tgggcgcgta
     4081 tctttcaatc ggcacaacgc tggcgatgtt ctgcggaggc attgtgcgct ggatggccga
     4141 ccgcgcggtg gagaaagcgg gtggaagcgc ctcagatatg gaaagcgaag tttctcctgg
     4201 ctcgctgtat gccagcggac tgattgcggc aggtggtgtg atggggttac tgggcgtcgc
     4261 gcgcaagctc tatgagacgg ccactggaaa cgagctgcac gtctggtttg cggaaggcag
     4321 tgttgtggac cgcctgctcc atcatcaggg agtttcgata gccgcctttg ttctgctggc
     4381 gttttcgctc tactacttcg cacgcaaacc gctgaaggag tagagcggca taccttacaa
     4441 cagtgcctct gcttcaagca cacgctggac ggcctcttcc ggcgtctttc ctacagcaga
     4501 aagatggccc attttgcggc ccttccgtgg ctggtgcttt tcatacagat gcaggcgaac
     4561 gccaggcacg cgcagcgcct gatcgaagcg tggctcgtgg ttgagccata aatcgcccag
     4621 aaggttcacg atagcggcag gcgcgacgac agaagtatcg ccaagtggga ggtcacagac
     4681 cgcccgcacg atctgctcaa actggctggt gacgcaggcg cgctcgctgg cgtgataact
     4741 gttgtgcgga cgcggggcaa gctcattgat cagcaaccgt ccatcgcgcg tgagaaacat
     4801 ttccacggcg agaacccctt cgaggtgcat cttgtctgcg atgtcgcgtg cgagcttctg
     4861 tgctttttct tccagcgaag agggaatgac tgaagggatc acgctccagg caaggatctg
     4921 gcgctcatgg tgatttgtgg ctgtcggaaa gctttttgtt tcgccgtttg gagcgcgggc
     4981 caccatcact gagatttcct tgtcgaggtc gagcgctttt tctgcaatgg cctgccgttc
     5041 gccgagggac ttccatgctt ccgcggcctc ggcaatgccc gaagatgcgt tctcaccaaa
     5101 cccgattttt gcctggctgc ggccgtcgta tccgccgcgc gcgcttttga cgaagagctt
     5161 tccgccgaga gcagaggctg cgctttgcag atcggcctcg ctgtggatgg cgcgatactc
     5221 gccgactgga aagccgttct ctgcgagcca gtttttttgc aggatgcggt cctgaatgat
     5281 ctgcatcata gcgatgccgg gacgcacggg cgcaaatttt gccgccgctt ccagactggc
     5341 aatggagacc tgctcgattt cgagcgtgac gacatcggag ccgcgggcga gatcggcggc
     5401 agcgcgggag tcatcccacc cgccttcaaa gcacgcatcc acaacaaagc gcgctgggca
     5461 cgacgggtca ggatccatga cctgcacgcg atacccaagc gagcgtgcgg ccatggcggt
     5521 catgcggcca agctgcccgc cgccaagaat gccgatggta gaaccgggaa gaagggcgct
     5581 cattgcggca gatcctcggc gagcacttca cgtttgcgtg cctcgcgcca ggcgcgcagc
     5641 cggtggcgca attccgtgtc agttgtggcg aggatttccg ctgcaaacag ggccgcattt
     5701 gccgctccgg gcttgccaat ggcgagcgtg cctacaggaa cgcctttagg catctgcacg
     5761 atggaaagca gcgaatctat gccgttcagc atggtggcag gaacgggaac gcccaatacg
     5821 ggaacgagtg ttttggcagc aagcattccg ggaaggtgtg ctgctccacc tgctccggca
     5881 atgatgacgc gcaggccgcg ttcttctgcc tgctcagcat attggaaaag ccaatcggga
     5941 gtgcggtggg ccgagaccac tcgtacttca taaggaattg agagttcttt cagcaggtca
     6001 acggcggctt ttaagatttc gtaatcgctt ttgctgccca tgaccacgcc gacgagaggg
     6061 gcgtgctcca tagagatgat tatagtggga ggggatttga ggaaggattg tataaaatgc
     6121 ggctgcccca tcctttgcgc ctgcatgggg agcaaaggat gggaaggaaa cacccttaca
     6181 gcttgattcc gatcgaggcg gcgaacttta ccaacgcggg ccagacggct ttcaggtcct
     6241 ggatggtcgc ggcatccagc gtaagattca gcggatttgc agctgcggcg ctaacatctt
     6301 cgacggcctt tgccactgta ccgagaagta cacccagacc tgcaatgaca tcgggatcca
     6361 ccttgctggt caggtctttt actccggtga gaaatttcag aacgtcttct gctgctactt
     6421 ctattccgtg ttcgacaccc aaaaggatat ttgccattta ctgtttctcc gtagtttgtt
     6481 ggtttatggt tgcgccaaag gccctgtcga ggtttgccgc aagaagatga gtgaagcgat
     6541 agagccatct gtaaaagcgg ctgtcgtccg cgcgcggagc gtccatggcg ctcacggctg
     6601 cggaaaaaat ccacagcacg cccaccccaa tccagaagaa ctggggattg ttggtagata
     6661 tgttcatagg ctgtttcctt gtgttaagag aaaagcaggt gctaaggctg cgtggccggg
     6721 tggtccatgt cttgcaggat ttacagcggt acgtttacaa acaccgcgct tgagaagcgt
     6781 gaataattcg gcggggtcga gctgtcatac atgcgcacgt aatactgctc catgacggcc
     6841 tcgcgcggaa cggtgaagtt gctcaccgga ctgcgaagca ccaaatctga atccgtgccc
     6901 gggccaaatg accagtcccg ccgccgcacc tcgaatccac caccggaagg tggcgtgatg
     6961 ttggtgctga tctggattgc ggaagagctg actgaggtca cgctcatctc tcgcagattg
     7021 tcgagcggcg ctgttgtctg cggttgctga ggaagccatg tatctgcggg aatagtggac
     7081 gatgttttga tggcaagcgg ttcggcccag tcatttgcaa agtagatgat gtatttgttc
     7141 agttgtggtg acgtattctg catctcaatc tgcactttgc gcacgactac gttggccgcc
     7201 attccagagg aagtggccga gatggcgagc aggtctccgg gccacacgtc gccctgcgtc
     7261 tcaaaattcc agcctgtgta agtacccttc catgctgctc cgcgacttga ggccagatcg
     7321 aggatggctc ttgccgcatt ctcgcaatcc acagaactga ggggtttggg gtgggtgaca
     7381 gtgcccatcc agcgggaggt tgccgggacc tgcgaagatg catcaggtga ggtagctgcg
     7441 ccgtttgcca gacgcgcgac agcccggcgc ctcgtccggt aggaaacgaa aatcagctca
     7501 ccctgttgcg gcacgctggt tgcataaaaa cgcagtttgc ctgtgcgctc gatccggcag
     7561 tctgcgccct gcgctgttgt tccgatacgt cgtgttactg cgctgcctcc gggaggaata
     7621 ctgatgaccc attccggccc ttgctgcgta attgagatgg tgcggatgct gcaaaaaaga
     7681 ctggcgctat tcaagggagc atacactccg gctgccggag cattcgtaat cacaccgtca
     7741 tagagaatga cgggagattg cgccacacca ttggtcgtgt cctggatttc cagcatgcaa
     7801 tgaatgttgc acggaatccc ggttccaccc cagagcttta cgactgtatc gtctaccgag
     7861 taatacgatt gcaggacgcg ctgcatttct tttgcgtaaa tacgtgtgcg tagggtatag
     7921 acatgtccgg aaacaggggt gaatgcattt cctgtaactg tgcctgatac aaatgcagca
     7981 acagaattac tgccgttgtt ctgtgtgacc tgaaacccgg caatacagtt ctgcgcgctt
     8041 acgctgccgg agtacaaccc gctcagaatt ccggtgctgg ctgctgtaaa ttgcagaccg
     8101 gtggcctcca ccatcaacga tccgccaagc tcgaagctgc tgattgcact gacaaatgtc
     8161 tgtccgtcat agccgttgcc gccggagcag gtaagcccat tcgcggtaag cgatacgtgc
     8221 tggcctgggt cttgtacttg ccacagg
//
LOCUS       DASRBV010000101         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_156501, whole genome shotgun sequence.
ACCESSION   DASRBV010000101 DASRBV010000000
VERSION     DASRBV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156501"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFA02_04065"
     CDS             <1..438
                     /locus_tag="VFA02_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZU09053.1"
                     /translation="GKTNAIGRPTTVIYANDLSVHSLLDAIRKGHVFIDLSASRDRLL
                     DVSAEDNGEKAVMGDTLAAPANSTIKIFAHIVACEGLRLRFLLDGKPVDSLAADLTQP
                     DQTIPLTLTADGKKHWLRPDVVTPEGKLILLGNPIYLNDSESK"
     gene            435..1508
                     /locus_tag="VFA02_04070"
     CDS             435..1508
                     /locus_tag="VFA02_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09054.1"
                     /translation="MISQDKFVTLSEVQSAKSKDLILAAIVAVLLFLSPALCAQNAAH
                     PGARSVMDAHNCYPYYEWWSDRIDRALSAGTPLAIEQDLAWYTDKKTGKSWSIVTHGL
                     PGYGNEPTMKQYFFERVRPIAEKALKEGNHGDWPIITLNLDFKTEEPDHLRAVLALLD
                     EYRDWITTATKTSDASKVQPLTVRPILVLTGESDAQEKVFYDEVPVGGQLLLFGAVHT
                     HEKDPMAAPEVLEPERANNYRRWWNNPWSVVEQGGQNHAGDWTKADNLRLRALVEHAH
                     QNGLWIRFYTLDGVDNKQDLSCHGWFSSYNFGSLNAAEVRWKAALEAGVDYIASDQYE
                     LLGKFIREHGGENQSVVRQQAGR"
     gene            1558..1995
                     /locus_tag="VFA02_04075"
     CDS             1558..1995
                     /locus_tag="VFA02_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZU09055.1"
                     /translation="MLERARLKFFETFVRTASRRRKEPEHLATGRRGELAAYFYLRRQ
                     GYIITARGWRSGHLRGDIDLIGWEGDTLCFIEVKTRTTRDVATPEAAVDEDKRRTLRR
                     VARHYMRGLPQQDIPARFDILSIYLEKEKAAEFELFRGAFDWS"
     gene            complement(2116..2832)
                     /locus_tag="VFA02_04080"
     CDS             complement(2116..2832)
                     /locus_tag="VFA02_04080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU09056.1"
                     /translation="MLATLATAGALTLAAGGYAYAAMWPTSQIFGRAIIGGPDAREFA
                     LTYDDGPNDPYTQQLLDVLAEHNVRATFFLIGRFVRQRPDIARAIHAAGHLVGNHTMT
                     HPVLLFQSPRRVLDELKNCNAAIEDALGGPVRYFRPPHGARRPDVLRTASHLGLKPVL
                     WNAMGYDWKPTTAEAVLANLQKGIRRNQHRGIGSNLLLHDGGQTGIGQDRSHSVTATA
                     SLLRSARYEGIRFVTADAWG"
     gene            3013..3660
                     /locus_tag="VFA02_04085"
     CDS             3013..3660
                     /locus_tag="VFA02_04085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZU09057.1"
                     /translation="MAKLFEQPRFIAVEGPIRVGKSSLAQLLAERIHARHIIEPENNP
                     FLDRFYQGEAGMAFAAQMWFLKERHEQLRQAKNTDGPVVSDYIFEKDKIFAYVNLGDA
                     ELALYNRYYQQLREDVPTPDLVIYLQATPEVLKQRLKRKGMAGERAISDGYIEQLAAA
                     YEHFFFHYTASDLLIVNTSDIDFVNNNRDLQLLLKRLSEPVKGTQYFLPLESASG"
     gene            complement(4187..>4676)
                     /locus_tag="VFA02_04090"
     CDS             complement(4187..>4676)
                     /locus_tag="VFA02_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09058.1"
                     /translation="IFDGKQGWEITYRGKRPLPQDQLDDFIRRRDHSIETVVKVWMKD
                     PNTIYLYEGQTLAERHLADQVTLISSTNDSVTIQMDADTHLPLRRTFQWRDPVYKDKN
                     EEVEEYDDYHTIDGIPTPFTITRFHNGDETNQRYVYKAAYNVPLPPDAFNVDALAAKI
                     KK"
BASE COUNT         1048 a         1225 c         1324 g         1079 t
ORIGIN      
        1 ggaaaaacaa atgccatcgg acgtccaaca acagtcattt atgcgaatga cctttccgtg
       61 cattcgctgc tcgatgccat ccgcaaaggg catgttttta tagacctgag cgcctcgcgt
      121 gaccgcctcc ttgatgtttc agccgaagac aatggcgaga aggctgtgat gggagatact
      181 ctggcagcgc ctgcgaactc caccataaaa atcttcgccc acattgtcgc ttgcgagggc
      241 ctgcgtctgc gttttctgct cgacgggaag cctgttgatt cgcttgctgc ggatttgact
      301 caacctgacc aaacgatacc acttacgctt acggcggatg gcaaaaaaca ctggcttcgc
      361 cccgatgttg tgacacccga aggcaagctg atcctgctcg gcaaccccat ctatctcaac
      421 gattcggaaa gcaaatgatc tcacaagata aatttgtcac cctgagcgaa gtgcaaagcg
      481 cgaagtcaaa agacctgatt cttgccgcga tagtcgctgt ccttcttttc ttaagtcctg
      541 ccttgtgtgc ccagaacgct gcgcatcccg gcgcgcgctc tgtcatggat gcgcacaatt
      601 gctacccgta ttacgaatgg tggtctgatc gcattgaccg cgccctttct gcaggaactc
      661 cgctggccat tgagcaggac cttgcctggt acaccgacaa gaagacgggg aaatcatggt
      721 ctatcgtcac gcacggcctg cctggttatg gcaatgaacc aacgatgaaa cagtatttct
      781 tcgagcgtgt gcggcccatc gcggagaagg cgctgaaaga gggcaatcac ggtgattggc
      841 ccatcatcac gcttaatctc gatttcaaga ccgaagagcc ggatcatctt cgtgctgtgc
      901 tggccctgct cgatgaatac cgagactgga tcacgaccgc aacgaagaca tccgatgcat
      961 cgaaggtcca gccgctcact gtccgtccta tcctcgtgct tacaggagaa tctgacgcgc
     1021 aggaaaaagt cttttatgac gaagtccccg tcgggggaca acttctgctc ttcggggctg
     1081 tgcataccca tgaaaaagac cctatggctg cgccggaagt cctcgaacca gaacgggcca
     1141 acaattaccg ccgctggtgg aacaatccct ggagcgtcgt cgaacagggc ggacaaaacc
     1201 atgcgggcga ttggacaaaa gcagacaacc tgcgcctgcg cgccctcgtt gaacatgcgc
     1261 accagaacgg cctctggata cgcttctaca cgctggacgg cgtggacaac aagcaggacc
     1321 tgagctgcca tggatggttc agcagctaca atttcggttc attgaatgcg gctgaagtcc
     1381 gctggaaggc cgcgctcgag gcaggcgtgg attatatcgc cagcgaccaa tacgagcttc
     1441 tcggaaaatt catccgggaa cacggagggg aaaaccaaag tgtagtgagg caacaggccg
     1501 ggcggtgaat tttcggggta tcaacggcgg tctcagcgaa aatcgttgca gatgcctttg
     1561 cttgaacgcg cacgtctgaa gtttttcgaa acattcgtcc gcacggcttc ccggcgccgt
     1621 aaggagccgg aacatctcgc cactggccgc cgcggtgaac tggccgcata tttctatctg
     1681 cgtcgtcagg gatatatcat caccgcccgt ggatggcgct cgggccacct gcgcggggac
     1741 attgacctga tcggatggga gggcgatact ctttgcttta ttgaagtcaa gacgcgcacc
     1801 acacgcgatg ttgccacacc ggaagcggct gtagacgagg acaagcgtcg aacattgcgg
     1861 cgtgtggcga gacactatat gcgcggactt ccgcaacaag acattccggc gcgcttcgat
     1921 attctttcca tttaccttga aaaagaaaaa gccgcggaat tcgaactttt ccgcggcgcg
     1981 tttgactggt cttaatgatg tatgcgaaca aatgaatctg gttcccagcc ggtttcctgg
     2041 cctgtgcggc ctgcctgcag aaagtgcccg gcatcttacc tcctgctgcc acggcgacgc
     2101 gactgcttca gacaactatc cccaggcatc tgctgttacg aagcgaatac cttcatacct
     2161 cgctgacctg agcagcgatg cagtggctgt aaccgaatgg ctgcggtcct ggccgatgcc
     2221 tgtctgtccg ccatcgtgca gtagaagatt cgagccaatg ccgcgatgct gattgcgccg
     2281 aatccctttc tgcaggtttg cgagcacggc ctcggcggtg gtcggcttcc agtcgtatcc
     2341 catcgcgttc catagaacgg gtttaagacc aagatggctg gccgttcgca acacgtcagg
     2401 acggcgcgct ccatgcggtg ggcgaaagta gcggacaggg ccgcccagcg catcttcaat
     2461 tgcggcgttg cagtttttca gctcgtccag aacacgccgg ggcgattgga acagcagcac
     2521 cgggtgtgtc atggtatggt tgccgacaag gtgtccggca gcgtgaatgg cgcgggcgat
     2581 gtcggggcgc tggcgcacga agcggccaat cagaaagaag gtcgccctaa cgttgtgttc
     2641 tgccagcaca tcgagcagtt gctgcgtgta tggatcgttg gggccatcat catacgtcaa
     2701 cgcgaattcg cgcgcatccg gaccgccgat aatggcgcgt ccaaaaatct gcgaagtagg
     2761 ccacatggcc gcgtaggcgt atcctccagc ggccagagtc agggccccgg cagtggcgag
     2821 cgttgcaagc atgctgcttc cagtttatgg cgcaattgcc ttctttgctt ccgcagaagc
     2881 gggcaagctg cgtcaagatg cgcccctcgc gagaagtgga agggcgcgaa caattttgaa
     2941 agcacgctct gctacaaaca gggagtggcg gccttcttca aaacgtggta tcttcgcttt
     3001 ttattcgatt ctatggccaa actgtttgag caacccaggt ttattgctgt ggaagggccg
     3061 attcgggtcg ggaaatcgtc tctcgcgcag cttcttgctg agcgaatcca tgcgcggcac
     3121 attatcgagc cggagaacaa tccttttctt gaccgctttt atcagggcga ggctggaatg
     3181 gcgtttgccg cgcagatgtg gttcctgaaa gagcggcatg agcaactgcg gcaggcaaaa
     3241 aacacggatg ggccggttgt ttctgactac atctttgaaa aagacaagat ttttgcgtac
     3301 gtgaacctgg gggatgcgga acttgcgctg tacaaccggt actaccagca actgcgcgag
     3361 gatgtgccca ctccggacct ggtgatttat cttcaggcca cgcctgaagt tctgaagcag
     3421 cggctgaagc gcaaaggaat ggccggagaa cgcgccatca gtgatggata tatcgagcaa
     3481 ctggccgcgg cttatgaaca cttctttttt cactacacgg ccagcgatct gctgatagtg
     3541 aacacctcag acattgactt tgtaaacaat aatcgcgacc tgcaattgct gctgaagcgg
     3601 ctctcggagc cggtgaaggg aacacagtat tttctgccgc tggaaagcgc ttcgggatga
     3661 aagagcttcc gcctaccgtc ctcgtttttt tgtaaattcg tcaaaggaaa tgatttaagt
     3721 aggttttcgt tttccgtgat gcagccgaag gctggaagcc gtgccccttc aaaacccgat
     3781 tgccggatga ttgcaatcgg ggaaaaggcg ggattgggcc caaccttttc ttcagtcgta
     3841 agaatgacgg agcctacata gataaaaaaa tataaaatat tcaaaataaa aggcttgcgc
     3901 atcaagcacg ggttaaagtc ctggaacgaa agggtttaca ggtaaagtac tgattctaaa
     3961 caggttttta cggaaaaacc tcagcgggcc tggctaacct ttgcattggc tgagattctt
     4021 cctccctccg gccgatatcg gtcgtttatt cacaaaaaag cccacctgtg gtgggccttt
     4081 tttgactctc tatttttatt ttatcattcg tgggaagatg tttcggacat tgctcgttgt
     4141 gacgagtaat ttttggttgt gcgtggtcct tcagaccgcg tgacgcctat tttttgatct
     4201 ttgcggcgag cgcatccaca ttgaaggcat ccggcgggag tggcacgttg taagcggctt
     4261 tgtagacata gcgctggttt gtctcatcgc cattgtggaa gcgggtgatg gtaaagggcg
     4321 tgggaatgcc gtctatggtg tggtaatcat cgtattcttc cacttcttcg ttcttgtctt
     4381 tgtagacggg gtcgcgccac tggaatgtgc gccgcagggg caggtgtgta tccgcgtcca
     4441 tctgaatggt gacggaatcg tttgtggatg agatgagtgt gacctggtcg gcgaggtggc
     4501 gttcggcgag ggtctggcct tcgtagagat aaatggtatt cgggtctttc atccagacct
     4561 tcacgacggt ctcaatggaa tggtcgcggc ggcggatgaa gtcgtccaac tggtcctgcg
     4621 ggagggggcg cttgccgcgg tatgtaatct cccaaccttg tttgccgtca aaaata
//
LOCUS       DASRBV010000102         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_258686, whole genome shotgun sequence.
ACCESSION   DASRBV010000102 DASRBV010000000
VERSION     DASRBV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258686"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..679
                     /locus_tag="VFA02_04095"
     CDS             35..679
                     /locus_tag="VFA02_04095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZU09059.1"
                     /translation="MLAELLRIHPDEPEPERVEYVVSCLRSGKVVGMPTDTFYGLAVD
                     PVNLRAVERIYEIKSRLKHKPLSLLIGTLEQAYGLARDVDANFDRLAERFWPGPLTLI
                     VRASSRLPLRVTANTGNVALRIPDAAIPRAVTKAFGLPVTATSANLQGAPECTHAACV
                     RDQIGDRIPLIVDGGPTGRSLPTTIVDLSEGNGRWQILREGAIATHEIALALHR"
     gene            742..1317
                     /locus_tag="VFA02_04100"
     CDS             742..1317
                     /locus_tag="VFA02_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZU09060.1"
                     /translation="MNTVLVLIAAAAAWSAWVYGQIRYYADRDEAQPANAIAVFGAAE
                     YDGRPSPVLRARLDHALELYQRGLAPLMITLGGGDVGDTHSEGGVGHDYLLAHGVPES
                     AIIAETQSNNTAESAARLAVIARANHLLRIIVVSDGTHLFRIHALCEKNGLHVFTSPR
                     PAAGNLDWQESFARMAHEILSYTAWRLHLHG"
     gene            1510..3126
                     /locus_tag="VFA02_04105"
     CDS             1510..3126
                     /locus_tag="VFA02_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09061.1"
                     /translation="MLSVNNVTMRYGSKVLFEDVTTTFTPGRRYGLTGPNGAGKSTFM
                     KILSGELEPQKGTVVRPKKIGVLRQDQYAFDAYRVIDTVIMGNKALWVAMEERDAIYS
                     KPELTDEDGMRLGELEGIVGEEDGYTAESDAAILLQGLDIPDELHERKMSELQGGQKV
                     RVLLAQALFGNPQALLLDEPTNHLDLDSIHWLVDFLQRFEGTLITISHDRHFLNSVCT
                     HTADIDYQTIITYTGGYDDMVLAKTQVRARLEAQNEQREKKIAQLNEFIARFSAGTRS
                     SQVTSRKKEVERLQTTELAKSNIQRPYIRFEQARPSGKHVLEIEGLSKSYGDHVVFNG
                     FSGAVMRGEKVCLIGRNGQGKTTMLKSLLAGFPELAEKDFSLDAGTVKWGHEAQIGYF
                     PQDHAGVIEHGMTVNEWLHQFDPKATTEDIRGILGQMLFRGEEGLKPTAALSGGEAAR
                     LIFCKLMLQKPNILILDEPTNHLDLESINALNIALQRYEGTVLLVTHDQDLIEEVGTR
                     IWHFEKGAIEDFKGPYEEYTAASGSRSFVG"
BASE COUNT          772 a          875 c         1035 g          760 t
ORIGIN      
        1 cgccatctta ggatttctct cgattggagt tgccttgcta gccgagcttt tgcgcataca
       61 tccggacgaa ccggagccag agcgcgtaga gtacgttgtt tcctgtctgc ggagcggaaa
      121 agtggtcgga atgccgaccg ataccttcta cggtctggcc gtggacccgg taaatctgcg
      181 ggctgttgag cgtatctacg agatcaaaag ccggttgaaa cataagccgc tgtcgctgct
      241 aattggcacg ctggagcagg cctacgggct ggcgcgcgat gtagacgcca actttgaccg
      301 tctggcagag cgcttttggc ccggaccgct cacgttgatc gtaagggcca gctcgcgttt
      361 gccattgcgg gtgactgcaa acaccggcaa tgtagcgttg cgtattccag atgcggcgat
      421 tccgcgcgcg gtgacaaagg cgtttgggct tccggtgaca gcaacctccg caaatctgca
      481 gggagcgccc gaatgcacgc acgcggcgtg tgttcgggac cagattggcg accgcatccc
      541 attgattgtg gatggaggtc cgaccgggcg cagccttccg acgacgattg tagacctctc
      601 ggaaggcaac ggacgctggc agattctgcg cgaaggggcg attgctacgc acgaaatcgc
      661 actggcgctg caccgttgag cgcggccatg attgttccgg gcacaaatct gaagcgaggc
      721 catgctggca gacggttcat tttgaatacc gttctggtgt tgattgccgc cgcagccgca
      781 tggagcgcat gggtttatgg ccagatccgc tactatgcgg accgcgatga agcacaacct
      841 gcaaatgcca ttgctgtttt tggcgctgcg gaatacgatg ggcggccttc tccggtatta
      901 cgggcgcgtc tggaccatgc gcttgagcta taccagcgcg gccttgcgcc actgatgatt
      961 actcttggcg gaggcgatgt aggcgatacg cactccgaag gaggggtcgg gcacgattat
     1021 ctgttggcac atggagtgcc agagtcagcc attattgcgg agacgcagag caacaacacc
     1081 gcagaatcag cggcgcggct ggcggtgatt gcgcgggcga accatctttt acgcatcatc
     1141 gtggtgagcg atggaacaca cctgttccgc attcacgcac tctgcgaaaa gaacgggctg
     1201 catgtcttta cgtcgccacg gccggctgcg ggaaacctcg actggcagga gtcatttgcg
     1261 cgcatggcgc atgaaatcct gagctacacg gcctggagac tgcacttgca tgggtgaaac
     1321 ttattctgcg cacaggagag tgtctgaaat caggcaaaat cctgagattt agtaaaatag
     1381 aagagaagtg ctgagaaaat agctttttcc ttgatcctgc ctcggcggaa caaccagcaa
     1441 cattccagag cgtgcatctt ttgtagtgtg aagactacgt gcgctttgcc tcaataacag
     1501 gaatagacga tgctttcagt caataacgtt acgatgcgct acgggtcgaa ggtcctcttt
     1561 gaggacgtga ccaccacttt tactcccggc cggcgctacg gactgaccgg gccgaatggc
     1621 gctggcaagt ccacttttat gaagattctt tctggtgaac tggagccgca gaagggcaca
     1681 gtggtgcgtc cgaaaaagat tggcgttctg cgccaggacc agtatgcctt tgatgcctat
     1741 cgcgtgattg atacggtcat tatgggcaac aaggcactgt gggtagccat ggaagagcgc
     1801 gatgccattt attccaaacc tgaattgacc gatgaagacg ggatgcggct gggcgagctt
     1861 gagggaattg tcggggaaga agatggatac acggctgaaa gcgatgcggc aattcttctt
     1921 caggggctgg acattccgga cgagttgcat gagcgcaaga tgagcgagct gcagggcggg
     1981 cagaaggtcc gcgtgctgct ggcgcaggcc ctgttcggca atccgcaggc acttctgctg
     2041 gacgagccta caaaccacct cgaccttgat tccatccact ggctggtgga ttttctacag
     2101 cggtttgaag gcacgctgat tacgatttcg cacgaccgtc attttctgaa tagcgtttgc
     2161 acgcacacgg cagacattga ttaccagacc atcatcacat ataccggcgg gtacgatgac
     2221 atggtgctgg caaagacaca ggtccgcgca cgtctggaag cccagaacga gcagcgcgaa
     2281 aagaagattg ctcagctgaa tgagttcatc gcgcgctttt ccgccgggac ccggtcaagc
     2341 caggtgacga gccgtaagaa ggaagtggaa cgcctgcaaa cgacagagtt ggccaaatcg
     2401 aacattcaac gcccttatat tcggtttgaa caggcgcggc catcgggtaa gcatgttctg
     2461 gaaatagaag gtttgagcaa gagctatggc gatcacgtgg tgttcaatgg atttagcggc
     2521 gctgtgatgc gcggcgagaa agtctgtctg attggccgca acgggcaggg aaagacgacc
     2581 atgctcaagt cgctactggc cggttttccc gagcttgccg aaaaggactt ttcactggat
     2641 gctggcactg tgaagtgggg ccatgaggcg cagattggat attttccgca ggaccatgcc
     2701 ggagttatcg agcacggcat gacggtgaat gaatggctgc accagtttga ccccaaggcc
     2761 acaacggaag acattcgcgg cattctggga cagatgctct ttcgcggcga agaagggctg
     2821 aagccgacgg cggcgctgtc aggcggagag gcggcacggc ttatcttctg caaacttatg
     2881 ctgcaaaaac cgaacattct gattttggac gagccaacca accaccttga tcttgagtcc
     2941 attaacgcgt tgaatattgc attgcagcgt tatgagggaa cggtgctgct ggtgacccat
     3001 gatcaggacc tgattgaaga ggtcggaacg cgcatctggc attttgagaa gggtgcgatt
     3061 gaggacttca aaggtccgta cgaggagtac accgcagctt caggatcgcg ttcatttgtg
     3121 ggatagttct gctcacgtgg gctgaggtca ttgtccaaca tcagttgtgt gcagggcgcc
     3181 gacatcccga agcgcatccc acacatcctc tggacgggcg atcgtaacaa tatctctgcg
     3241 gatggatgcc atatgattca gttccacctc tgaatagatc tttgaccgcg gcaaatactt
     3301 acacccgcca gtggtctggc cgccagtttt tgattcgctg cttgatttct tttcctttca
     3361 ggttatgggc cagggtttct tctaccaatg cagggagttc ttcatcggaa gcaaagcgca
     3421 gggccacgag ctgtctttct gc
//
LOCUS       DASRBV010000103         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_461019, whole genome shotgun sequence.
ACCESSION   DASRBV010000103 DASRBV010000000
VERSION     DASRBV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461019"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFA02_04110"
     CDS             <1..522
                     /locus_tag="VFA02_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09062.1"
                     /translation="TDQLSGVVNGKAVSFQATSDPTGRQFLLDISANGQKQQNKIDRD
                     QNSVLLTDLDPAGYMLLMQVALRNPKDAWAIIPKENGFLVPVKFTTLEDTKAAYNGQT
                     MMVHHTSAAVSTQNAITIELFYSGDGQLLEADLPEQNFDVIREGFRLIHRPKPPAPPH
                     GEAPAPPPQNQQQ"
     gene            complement(524..2137)
                     /locus_tag="VFA02_04115"
     CDS             complement(524..2137)
                     /locus_tag="VFA02_04115"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZU09063.1"
                     /translation="MNRGRLLRGLFAATAAFCFAISASAQDTVTVETGKLKGATADGV
                     TSFKGIPYAAPPVGALRWRPPQPAEKWSGVRDASHYGHDCMQLPFPSDAAPLGATPAE
                     DCLVLNVWVPEHRTGKLPVMVWIYGGGFVNGGSSPAVYDGSQFARKGIVFVSFNYRLG
                     RFGFFAHPALTKESKDGLLGNYGFMDQIAALQWVQRNIQAFGGDPRQVTVFGESAGGF
                     SVHMLMTSPLANGLFQRAIVESGGGRLMLNSTGLHEGMHGRPSAEEMGVAFAKSKGIP
                     GDDAAALKKLRALPAEEIVDGLNMASMGNAESTYSGPMIDGKLVIGQTQTLYEQGKYQ
                     HVPFIVGANTMDIGFSHAKTVDELFAPFGPNAKAAEEAYDANGTAPPQAIFFLGMKVA
                     ADAFMVEPARFVAQTFSSQGNPVWEYRFGYVAQSMRDKWPGAPHATEIPFVFDTVKAK
                     YGAELTSQDEKTAQQVNEYWANFAKTGNPNGAGLPNWPQYNTSQDVLMDFSLDGPAAK
                     ADPWKKRLDLTEELVRQQEKSASERQQSK"
     gene            complement(2230..3279)
                     /locus_tag="VFA02_04120"
     CDS             complement(2230..3279)
                     /locus_tag="VFA02_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HZU09064.1"
                     /translation="MPIPTEPIGSIPRPASLIQAMAASGHAELENAYADALRDTMQRF
                     EQTGSPIITDGEQTKPSFATYPLSGLENLAPNGVVIPFADGHVRQLPRLTAGPFRYGI
                     HAEKYLRAARAYTKLPLKQAVISASALSLLYPSNGIPGYTREAFLEDLLNEAEADIRG
                     ALDAGAAVVQIDFTEARLSLKLDPSGGLLRSFIALNNQVLERFSPEERKRIGVHVCPG
                     GDLDSTHSADIPYTSLLPELFQLKAGRFYLQMASEPDKAPALRTVKQLLGPKQLVFIG
                     VIDPIHPKIETPEQVCERVLEAASILPVEQLGTTDDCGFAPFADDTSTARETAFGKIR
                     SRVVGTALAARELGL"
     gene            3365..>3825
                     /locus_tag="VFA02_04125"
     CDS             3365..>3825
                     /locus_tag="VFA02_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZU09065.1"
                     /translation="MPTQAEKAAAFRALHDRPEPFVIPNPWDAGTARILTALGFEALT
                     TTSGGLAITLGRRDGTASITRTEALENASLIVQATHLPVAADLENGYGHSPEAAAETI
                     RLAAEAGLVGGSIEDATGDPSSPIYEFNHAVERVAAAAETARALPFPFMFVA"
BASE COUNT          823 a         1204 c         1009 g          789 t
ORIGIN      
        1 accgaccagc tcagcggagt cgtaaacgga aaggccgtca gcttccaggc cacttctgat
       61 ccgacagggc gccagtttct gctcgacatc tccgccaatg ggcaaaagca gcaaaataaa
      121 attgaccgcg accagaactc ggtcctgctc accgacctcg atcctgcagg ctacatgctt
      181 ttgatgcagg tggccctgcg caatcctaaa gatgcgtggg ccatcattcc caaagaaaat
      241 ggatttctcg ttccagtgaa gttcacaacg ctcgaagata caaaggccgc atacaacggt
      301 cagaccatga tggtccacca caccagcgcc gccgtcagca cgcaaaacgc catcaccatc
      361 gagcttttct actcaggtga tggacaactg ctcgaagccg acctgcccga gcaaaacttt
      421 gatgtgatac gcgaaggctt ccggctcatc catcgcccga agcctcctgc tcctccgcac
      481 ggagaggccc ctgcgcctcc tccgcagaac cagcagcaat agctcacttc gactgctgcc
      541 gttcggatgc actcttctcc tgctgccgga ccagttcttc ggtcaaatcc agccgcttct
      601 tccacggatc ggccttcgca gccggaccgt ccagtgagaa gtccatcagc acatcctggc
      661 ttgtgttgta ctgtggccag ttgggcagcc ctgccccgtt cggattgccg gtttttgcaa
      721 agttggccca atattcgttc acctgctgcg ctgttttctc gtcctgcgaa gtcagttccg
      781 ctccatactt tgccttgacc gtgtcgaaga caaaaggaat ctcagtggca tggggcgcgc
      841 ccggccactt gtcacgcatg gactgtgcga catagccaaa gcgatactcc cacactggat
      901 tgccttgcga ggaaaaggtc tgtgccacga accgcgctgg ctcgaccatg aatgcgtctg
      961 ccgcgacctt cattcctaag aagaagatgg cctgaggagg tgctgtgccg ttcgcatcgt
     1021 aggcttcctc ggctgccttt gcgttggggc cgaacggcgc aaacaactcg tccaccgttt
     1081 tcgcatgaga gaagccgatg tccatcgtat tggcgccaac gatgaatggc acatgctggt
     1141 acttaccttg ttcgtagagt gtctgtgtct gcccaatcac gagcttgcca tctatcatcg
     1201 ggccggaata cgtggactcg gcattgccca tggaagccat gttcagaccg tccactatct
     1261 cttctgcggg aagcgcgcgc agttttttga gcgcggcagc atcgtctcct ggaatgcctt
     1321 tgcttttggc gaaggcaacg cccatctctt ctgctgaggg acgcccatgc atcccctcat
     1381 gcagcccggt gctgttgagc atcagccgcc cgccgccgga ttccacaatt gcccgctgaa
     1441 acagcccgtt tgccagcggc gaggtcatca gcatatggac ggagaaccct ccggcagact
     1501 caccgaagac cgtgacctgt cttgggtcgc ctccgaaagc ctgaatattg cgctgcaccc
     1561 attgcaacgc ggcaatctgg tccataaagc cgtagttacc gagcagtccg tctttactct
     1621 cttttgtgag cgccggatgc gcgaagaaac cgaaccgccc caggcggtag ttaaagctga
     1681 caaagacaat tcccttgcgc gcaaactggc tgccatcgta gaccgcgggc gagctgccac
     1741 cgttcacaaa accaccgcca taaatccaga ccatgaccgg aagctttcca gtccggtgtt
     1801 ccggtaccca gacattcaga acgagacaat cttcagcagg tgttgcgccc agtggcgccg
     1861 catcactggg gaagggaagc tgcatacagt cgtgcccata gtgtgaagca tcgcgcacgc
     1921 cgctccattt ctccgccggt tgcggaggac gccagcgcag cgccccaaca ggaggagcag
     1981 catagggaat gcccttgaat gatgtgactc cgtctgctgt ggcccccttg agcttgcctg
     2041 tctcgaccgt caccgtgtcc tgcgcgctgg cgctgatggc aaaacagaaa gcagcggtcg
     2101 ctgcaaaaag cccgcgtaac aaccttcccc ggttcataat gagtatgccc cctctgaaat
     2161 acgggagcat tgtatcaatt aacttctggc ctgaatctgg ccggacgctg gtgccccaca
     2221 cacatcccgt cagagaccaa gctcgcgtgc tgcaagtgct gtccccacga ctcttgatcg
     2281 tattttgccg aaggccgtct cccgcgccgt ggaagtatcg tcggcaaatg gcgcaaaccc
     2341 acagtcatct gttgtcccaa gctgctctac aggcagaatg gaagcggctt ccagtacgcg
     2401 ctcgcacacc tgctccgggg tttcaatctt cggatgaatc ggatcaatca ctccgatgaa
     2461 gaccaactgc ttcgggccga gaagctgttt taccgtgcgc agtgccggcg ctttgtccgg
     2521 ctcgctggcc atctgtaaat agaaccgccc tgcctttaat tgaaacaact ccggcagcag
     2581 agacgtataa ggaatgtccg cgctatgggt cgagtccagg tcgcctccgg gacaaacatg
     2641 caccccgatg cgcttccgtt cttcaggaga aaagcgttcc agcacctgat tgttcagtgc
     2701 aatgaagctg cgcagcaaac cacccgaagg gtccagtttc agcgacaatc tggcctcggt
     2761 aaagtcaatc tgcactacgg ccgcgcctgc atccagcgcg cctcgaatat cggcttcggc
     2821 ctcattcagc aggtcctcca gaaaggcctc tctcgtgtaa cccggaatgc cattggacgg
     2881 atagagcaga ctgagtgcag acgcagaaat cactgcctgc ttcagcggca attttgtata
     2941 cgcgcgtgcc gcccgcaggt atttctcggc atggatgcca taacgaaaag gccccgccgt
     3001 cagtcgtggc aactggcgaa cgtgcccgtc tgcgaatgga atcaccaccc cattcggagc
     3061 aagattctcc agcccggaaa gaggatacgt cgcaaagctg ggcttcgtct gctctccatc
     3121 cgtaatgata ggagaacccg tctgctcgaa ccgctgcatc gtatcacgaa gcgcatcagc
     3181 atacgcattc tcgagctctg catggccgga agcggccatt gcctggatca gcgaagctgg
     3241 acggggaata ctgccaatgg gttctgtcgg aatcggcata tgctgcggag tctaccgttg
     3301 cgcaaacgaa tgagcatcca tcgaaaggtg gatttgcagt accctgtttc ggagaggacc
     3361 acccatgccc actcaggccg agaaagctgc tgcatttcgc gcgctccatg accgcccgga
     3421 gccattcgtc attcccaatc cgtgggacgc aggtaccgcc cgtatcctga ccgcgcttgg
     3481 ctttgaggcc ctcaccacca ccagcggagg actcgccatt accttgggcc gccgcgacgg
     3541 aacagccagc atcacccgca ctgaagccct cgaaaatgcg agtctgattg tccaggcgac
     3601 acatcttcct gttgccgctg acctcgaaaa tggatacggc cactctccgg aggccgccgc
     3661 tgaaaccata cgtctcgcag ccgaagcagg gcttgtcggc ggttcgatcg aagatgctac
     3721 cggcgatcca tccagtccaa tttacgaatt caaccacgcc gtcgagcgcg tggccgcagc
     3781 cgctgaaacg gcccgcgcgc tgccgttccc atttatgttt gtggc
//
LOCUS       DASRBV010000104         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_614017, whole genome shotgun sequence.
ACCESSION   DASRBV010000104 DASRBV010000000
VERSION     DASRBV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614017"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2101
                     /locus_tag="VFA02_04130"
     CDS             233..2101
                     /locus_tag="VFA02_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HZU09066.1"
                     /translation="MHRKKALCLAGLILTIAASARAQYAELSPQRIHQIEAMLPAAPA
                     GFGRPCSDRGAWQPLAAQLASQVSRANALLTQPFPAWSDDAYLNFRRTGDRLVGEKMM
                     HARQDWLPTLVLAECAEGRGRFLSRIAMVLDELASQKSWTLPAHDGNLDSFSGRHYFV
                     ELNSADLAHTIAETLYLLGPQLPEATRRHMMDALEQRIFAPMRESFSTGKGNAWLHYK
                     MNWNAVCLDGVTGAALAVLPNRKDRALFAAAGEHYSQYYLEGFQNDGYGVEGMGYWNY
                     GFSHFVKLRETLWQATQGKIDLFASTKAKKIALFGAQFPMFPDNVASFEDAHYMLGPG
                     PGLMQYVERVFGLTASTPNADSGQIEATGLKTLTWIVMTLFPNHAAELKNAIPGGDTV
                     GLRTYYPESQVLVSRPQPGGKLAITIKGGGNTTHSHNDIGSFVIGLGATQPVGDPGGP
                     SYYTAETFSKNRLQSRLLNSYGHPVPVIDGHLQLDATKVTAQIMDTKFTDKQDSITIN
                     ATTAYDTPLLRKYLRTMHYSREGAGSIEIEDIFDLTGPADIEESLPTHGSWQQVDNKT
                     LEFTADEEHLRVVIDAPGTLAFSADKVDDYGNPFTRVAARVHLEHSGSVKMVFTPE"
BASE COUNT          528 a          706 c          598 g          462 t
ORIGIN      
        1 caatccaacg caggctcagc ccgcctgcaa gataaaacaa aaccagatcg cggaaagtaa
       61 gctctcgcgc aaggccggac gtggctgctt tctgtggcat gcaggcttcc gcgccagcat
      121 actggttgcc ctgacgcgaa gaccagagtt atttcgcagg gcggtacaac gctgcgtcct
      181 ccataaacct gcattgataa cctgaatgcg cccgcaaaag gagacccaac acatgcatcg
      241 aaagaaagca ctctgccttg ccggactcat tctcaccatc gctgcttctg cccgcgcaca
      301 gtatgcagag ctttcgcctc agcgcatcca ccagattgag gccatgctgc ccgccgctcc
      361 tgccggattc ggacggccct gctctgaccg cggcgcatgg cagcccctcg ctgcccagct
      421 tgcttcacaa gtcagccggg caaatgcgct cctcacccag ccattcccgg cgtggagcga
      481 cgatgcttat ctcaacttca ggcgcacagg agaccgcctc gttggcgaga aaatgatgca
      541 cgcgcggcag gactggcttc ccacactggt gctggcagag tgcgccgaag gacgtgggcg
      601 gttcctctcc cgtatcgcca tggtgctcga tgagcttgcg tcgcagaagt cgtggacatt
      661 gcctgcgcat gacggcaacc tcgacagctt cagcggcagg cattacttcg tggagctgaa
      721 ctcggccgat ctggcgcaca ccattgcgga aaccctctat ttactggggc cgcagttgcc
      781 tgaagcaacg cgccggcaca tgatggacgc tctggaacag cgcatctttg ctcccatgcg
      841 cgaatcgttc tcgacaggca agggaaatgc ctggctccat tacaaaatga actggaacgc
      901 tgtttgtctg gacggggtga caggcgcggc gctcgctgtt ctgcccaacc gcaaagaccg
      961 cgctctcttt gccgctgccg gagagcacta ctcccagtat tatctcgaag gttttcagaa
     1021 tgacggatac ggtgtagaag gcatgggcta ctggaactat ggcttctcgc attttgtgaa
     1081 gttgcgcgag acgctgtggc aggccacgca gggcaagatt gacctcttcg ccagcacaaa
     1141 ggcaaaaaag atcgcgctct ttggcgcaca attccctatg tttccagata acgtcgcttc
     1201 ctttgaagat gcgcactaca tgctcgggcc tggtcccgga ttgatgcaat acgtcgagcg
     1261 cgtctttggc ctgacagcat ccactccgaa tgctgactca ggacagatag aggccacagg
     1321 actcaagacc ctcacctgga tagttatgac actctttccc aaccacgccg ctgaacttaa
     1381 aaatgccatt ccaggcggag ataccgtggg cctgcgcaca tactatccgg aatcgcaggt
     1441 gttagtgtcg cgtccgcagc ccggcggcaa actcgcaatc accatcaaag gcggaggcaa
     1501 cactacccac agccacaacg acattggctc tttcgtcatc ggcctcggcg caacgcagcc
     1561 ggtcggcgat cccggaggcc cgtcgtacta caccgctgag accttcagca aaaacagact
     1621 gcaatcgcgc ctcctgaatt cttacgggca tcccgtgcca gtcattgacg gccatctgca
     1681 actggacgca accaaagtca cagcgcagat catggacaca aagttcactg acaagcagga
     1741 ctccatcacc atcaacgcaa ccactgccta tgacacgccc ctcctgcgaa agtatctgcg
     1801 caccatgcac tattcacgcg agggagcagg ctcgatcgag atagaagaca tctttgacct
     1861 gaccgggcct gcggacatcg aagaatcatt gccaacccat ggaagttggc agcaagtaga
     1921 caataaaaca ctcgaattta ctgccgatga agagcatctg cgagtcgtga tagacgcgcc
     1981 gggaacgctg gccttctccg ctgacaaggt ggacgactac ggcaatccgt ttacacgtgt
     2041 tgctgctcgg gtgcatctgg aacactccgg cagcgtgaag atggtcttca ctccagaata
     2101 ggcaatcact gttcaaacac ggcttctata aaaaagactc cggaaacttt tcgcctccgt
     2161 ctgctgttct tttcaccatg acatggaggt ggaacaaatg agattcagca ccgcgggact
     2221 tcttttgctt gccgctcttg cgcttcttgg cggatgcgcc agccatccgg catattatgt
     2281 tgcgcctccg ccac
//
LOCUS       DASRBV010000105         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_562644, whole genome shotgun sequence.
ACCESSION   DASRBV010000105 DASRBV010000000
VERSION     DASRBV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562644"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFA02_04135"
     CDS             complement(<1..939)
                     /locus_tag="VFA02_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09067.1"
                     /translation="MIPRLRHDFNQRFRPEVYRKMLHSLDACTRTHVKLPIAETPCFF
                     PKALLEEMAATGAGAIQKLVGNEELLRASRAAIPPQCRVADEAPHPHFAAADFLLSQE
                     TDGKTVLKLAGLHASPLTFGFQFVLNEVYRSAFELDSTLEHLFGGHTESSFWKLMERV
                     VLRGHQPEHVVLAGTNLQDREFFPDVQVIAERLGVEVEELASLEPEGTRLYYRRSGAR
                     VPIYRIYGYFHPAEIGCGQMELLFDPADSYDVEWAGHPNWCFHLGGSLARQTAKTECP
                     DSSVMIEAPHGKLKPEVRILYLWPEAGRLEAILAQVR"
     gene            1142..2275
                     /locus_tag="VFA02_04140"
     CDS             1142..2275
                     /locus_tag="VFA02_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU09068.1"
                     /translation="MSQRPSPLLLSALYFGFMLTGIGTTLLGCALPALSTDWRLTDSS
                     AGLLFAAQFVGSSSGALLVQSRLDSSILRGYSLIIAGGILISLLRGHLAPLFFVVFGL
                     GLGQAMTATSMTFGRIYSSSRGASLSLLNAAWGFGAVICPWIASFWARFRPSDWLFLG
                     IALASLPPFFYLVLHRDSLATHAEEAAQSTSRQADLSLLIPLAAFAFLYVGVESSVGG
                     WMMTYIHRLPVSGEVLAPVITSLFWIALLCGRTAAPAVLKRMTEPGLLTASLLVALAS
                     TGLLLLSHTSFSSLACATLIGLTLAPVFPLCISRLLGLTDKPSQSRWVFALSGLGGAV
                     LPWMTGQLASFQGSLRVGLLVPLFGTAIMLLLHLRTRPSAQTS"
     gene            complement(2283..4805)
                     /locus_tag="VFA02_04145"
     CDS             complement(2283..4805)
                     /locus_tag="VFA02_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HZU09069.1"
                     /translation="MKSKMWIGCGMVAVLSCIAAAQELKPTTIPLDQIDPARKITAPR
                     LQSETHKPLPEEYIWTQEDAISNDALLSYRRPGRNSETAPHYFRRVFTLDRVPPDATL
                     YIAGPRSVEVYVNGQLVGHDEANLDAPIGMQMFTVDVGNALREGQNIIAIEAVRGPGV
                     NGFTNSRFTMQLAAGKAMVVKLVGASRGADTPVLVISNKDWKASLHTAEGWQKADFDD
                     ASWKAADSLGPIEGSIDLFQWNADAGMYNWPGYEGISPFLAQAPLEPVSVEHVYGGVG
                     AVHNADALKGTSAGNEFTVDLPSRQVEDTQAPQVLLDFGRDANGRLHFISDSDRPAVV
                     TVQYGESEDEALKQPYLGMNAVYVPAHGSAYGPKSAFRYALLRFVEGGEGLRFKSINL
                     DLIYYPVKYQGFFESSDPKLNLMWTIGAYTAHLCMQDDIWDAPKRDRGRWMGDLDVSG
                     RTINDVFADHFLMEDTLNRLIGPAPVRQHVNGIAGYSAFWITGEAEYYRHFGNKQQLM
                     SFHDRLIQLMHYMEKELDGRNLYVNKTGSWPFVDWSPELNGDTPESRFGTQLEFIAAF
                     DQGVYLLKEMGDTANAEQFTRELDGLKAAAREYLLDRKTNAYSSRWQLNAMAVLSGLA
                     PVSQYADIWNASLSSVGKIKYNAFIITPYYNYYVISAMAKMGHRQEALNWIRQYWGGM
                     VDEGATSFWEGYDPAWYKNDAHGSLQADNMSGYRVSLAHGWSSGVTPWLMEEVLGIHA
                     TGPGFSTVDIRPDLIDLDWAKGGEPTPHGMLDVSLKKQGSGTSIAIELPAEVEAHVSV
                     PVSSTGQQVFVNGKAVKSESVEDGARAVVVLREAGHYEIAAK"
     gene            complement(4802..>5842)
                     /locus_tag="VFA02_04150"
     CDS             complement(4802..>5842)
                     /locus_tag="VFA02_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HZU09070.1"
                     /translation="GLMTRDLLAVLHQAPLSSIVWTFIFGVLWGFGGLTFGLTMRYLG
                     MSLGMAVALGYTAAFGTLMPPIFRGQFASEVLGTRSGIIILIGVVVCLAGIAFAGAAG
                     ISKEHELSEEEKRAAIEEFDLKRGLLVATFCGIMSACFAYGLAAGDPIKAITVQHGTS
                     PLWQGLPVLVVLLAGGFTTNFVWCVILHIRNRTGYQYLALQTWEYFPDRNEEPVIETA
                     IDAPGVEVATRTMTAVEQQGKVPILANYLFSALAGTTWYMQFFFYTMGETQMGRFKFS
                     SWTLHMASIIIFSTLWGIALREWKGVSLRTRILVACTLIVLVGSTVIVGYGNYLGVAT
                     TSTSASAQGASH"
BASE COUNT         1238 a         1789 c         1469 g         1346 t
ORIGIN      
        1 gcggacctgg gccaaaattg cttccaggcg tcccgcttct ggccagagat aaagtattcg
       61 gacttcaggc tttagctttc cgtgaggcgc ttcgatcatc acggaagagt ccggacattc
      121 tgttttcgcc gtctgcctgg ccaggcttcc acccagatga aagcaccagt ttggatgtcc
      181 tgcccactca acatcatagg aatcagcagg atcaaacaat agctccattt gtccgcagcc
      241 tatttctgct ggatggaagt agccatagat ccggtaaatg gggactcgcg caccactcct
      301 ccggtaataa aggcgagtgc cttcgggttc gagactggcc agttcttcca cttcaacccc
      361 aagccgctct gcaatgactt gcacatccgg gaagaattcc cgatcctgga gatttgttcc
      421 tgcaagcact acatgctccg gctgatgccc gcgcaggacg acccgctcca ttagcttcca
      481 aaagctggac tccgtgtgcc cgccaaacag atgttccaga gtgctgtcca gctcaaatgc
      541 agagcgataa acctcattca gcacgaattg gaagccgaac gtcagaggac tggcatgaag
      601 acctgccaat tttaggaccg tcttcccatc cgtctcctga gagaggagaa aatcggccgc
      661 cgcaaagtgc ggatgcgggg cctcgtcggc cacgcgacac tgcggcggaa tcgcagcacg
      721 cgaggcacgc aggagttcct cgttcccaac cagcttctgg atcgcgcctg cgccagtggc
      781 ggccatctct tccagcaatg ccttcgggaa aaagcaggga gtttctgcga tgggcagctt
      841 tacatgcgtg cgcgtacacg catccagact atgcagcatt ttgcggtaca cttccggacg
      901 gaagcgctga ttgaagtcgt gcctgagccg cggaatcatg gcatccggcc tgcaatgctg
      961 gccccggaac gcacgatctc cgggccggga accagaccaa acaagatacc gcaaatcacc
     1021 cggctttggc gcgtggggtt tctgatgatg aaaataaatt ttctatcatg ctttttatgc
     1081 gcgtattttg ccacggaaaa catggcggct tcgcgcccgg tctgctatat aagacgaagc
     1141 catgtcgcaa aggccgtctc ctctgctcct ttctgctctt tatttcggat tcatgcttac
     1201 gggcattgga acgacactgc ttggctgcgc cttgccagcg ttgagcacgg actggagact
     1261 caccgacagc tccgcgggcc ttctgtttgc cgcccaattc gtcggttcat cgtctggagc
     1321 gctgctggta cagtcgcgcc tcgattccag tattttgcgg ggctattccc tcatcatcgc
     1381 agggggcatt ctgatttcac ttttgcgcgg ccatctggcc ccgctgttct tcgtcgtctt
     1441 cggccttggc ctgggtcagg caatgacggc gaccagcatg acgttcggcc gcatctatag
     1501 ctcaagccgg ggagcatcct tgtcccttct gaatgctgcc tggggattcg gcgcagtcat
     1561 ttgcccgtgg attgcttctt tctgggcccg ctttcgtccg tcggattggc tgtttctcgg
     1621 aattgcgctg gcgtcgctgc caccgttctt ctatctcgta ctgcaccggg actctctggc
     1681 cacgcatgcc gaggaagccg cccaatctac cagcagacag gctgatctct cccttctgat
     1741 tccgctcgct gccttcgcct ttctttatgt aggggttgaa tcgtcggtgg gcggctggat
     1801 gatgacctac attcatcgcc tgccggtgtc cggtgaggtg cttgcccctg tcatcacatc
     1861 tttgttctgg atcgcccttc tctgcggcag gacagctgcg ccggccgtgc tgaagcgaat
     1921 gactgagccg ggcctgctga ccgcttcgtt attggtggcc ctagcaagca ctggactgct
     1981 gctcctcagt catacctctt tttcaagcct tgcctgcgcc acactgattg ggctgactct
     2041 cgctccagtc tttccgctct gtatttcgcg gctgctcggc cttaccgaca aaccatctca
     2101 gtcgcgatgg gtttttgcac tcagcggatt gggaggcgct gttctgccct ggatgacagg
     2161 acaacttgcc tcctttcaag gctcgctcag agttgggctg ctggtgccgc tgttcggaac
     2221 agcaattatg ctgttgcttc atctgcggac acggccttct gctcaaacaa gttaatcatg
     2281 gcctattttg ctgcaatctc atagtggccg gcctcacgaa gcaccacaac cgctcgcgca
     2341 ccgtcctcta cgctctcgct cttcactgct tttccattta cgaagacctg ctgcccagta
     2401 gaagaaaccg gtacggacac atgcgcttct acttctgcag gaagctctat cgcaatgctt
     2461 gtgccactgc cttgtttctt cagcgagaca tccagcattc catgcggcgt tggctctccg
     2521 cctttggccc aatccaggtc aatcagatcg ggacgaatgt ctacggtaga aaaacctggc
     2581 cctgttgcat gaatacccaa cacttcttcc atcagccacg gtgtcacgcc gctcgaccat
     2641 ccatgcgcca ggctcacgcg atagcctgac atattgtctg cctgaagcga cccatgcgcg
     2701 tcgttcttat accacgcagg atcataccct tcccagaagc tcgttgcgcc ctcatccacc
     2761 atgccgcccc agtactggcg tatccagtta agcgcctcct gtctgtggcc catcttcgcc
     2821 atcgcgctaa tgacgtaata gttgtaataa ggcgtgatga tgaacgcgtt gtacttgatt
     2881 tttcctacac tcgaaagcga tgcattccag atgtccgcgt actgcgatac aggcgccagt
     2941 cctgacagca ccgccatggc attcaactgc cagcggctgc tgtaagcgtt cgttttcctg
     3001 tccagaaggt actcgcgcgc cgccgccttc agtccgtcaa gctctcttgt aaactgctca
     3061 gcgtttgccg tatcgcccat ctctttcagc aggtacacac cctgatcaaa tgccgcaatg
     3121 aactccaatt gagtaccgaa gcgcgattct ggcgtgtctc cattcagctc tggcgaccaa
     3181 tctacaaatg gccatgagcc ggttttgttc acatacagat tgcgcccgtc cagctctttt
     3241 tccatgtagt gcatgagctg gatgaggcga tcgtggaagg acattaactg ttgcttgttg
     3301 ccaaagtgcc gataatattc ggcttctcct gtaatccaga aggccgaata gccggcaatt
     3361 ccatttacat gctggcggac cggggcaggc ccgatgaggc gattgagtgt atcctccatc
     3421 aggaagtggt cagcgaaaac gtcgttgatg gtgcgcccgc ttacatcgag atcgcccatc
     3481 cagcgaccac gatcacgctt cggcgcgtcc caaatgtcat cctgcataca gagatgcgcc
     3541 gtgtacgcac caatcgtcca catcaggttc agctttggat cagaagattc aaaaaatccc
     3601 tgatacttca ccgggtagta aatcaggtcg agattgatgg acttgaagcg aagcccttcg
     3661 ccgccttcca caaaacgaag cagcgcataa cgaaaagcgc tcttcggccc ataagccgaa
     3721 ccatgcgccg gaacataaac tgcattcatg cccagatacg gctgcttcaa cgcttcgtct
     3781 tccgattcgc catattgcac cgtcaccacc gctggcctat cagagtcaga aatgaagtgc
     3841 aatcgtccgt ttgcatcgcg gccaaagtcg agcagaacct gcggggcctg tgtatcttcc
     3901 acctgccggg atggcaggtc caccgtaaac tcgttacctg cacttgttcc ttttaatgca
     3961 tcagcattgt ggaccgctcc cacccctcca tacacatgct caacactcac aggttccagc
     4021 ggtgcctgcg ccagaaaagg cgaaatccct tcataaccgg gccagttgta catgcctgca
     4081 tcggcattcc actggaaaag atcaatcgag ccttcaattg ggccaagact gtcggcggcc
     4141 ttccatgatg catcgtcaaa atctgctttc tgccagccct ctgccgtgtg cagagacgct
     4201 ttccagtctt tgttgctgat gacaagcacc ggagtgtccg cgccacgaga cgctcccacc
     4261 agtttcacga ccatcgcttt tccggcagca agctgcatgg taaaacggct gttggtaaag
     4321 ccattcacgc ccggtccgcg caccgcttca atcgcgatga tgttctgtcc ctcacgcaga
     4381 gcgttgccca cgtccacggt gaacatctgc atgccaatcg gtgcatccag attggcttca
     4441 tcatgcccca ccagctggcc attcacataa acctcaactg aacgcgggcc tgcgatgtaa
     4501 agcgtcgcat caggagggac cctgtcgagc gtaaaaacgc gccggaaata atgcggcgca
     4561 gtctcgctgt ttcttcccgg ccttctgtag gacaacagcg catcattgct gatggcgtct
     4621 tcctgtgtcc agatatattc ctccggcagc ggtttatgcg tttctgattg caaccgcggg
     4681 gcggtaatct tacgcgcagg atcaatctga tcaagaggaa tcgtcgtcgg cttgagttcc
     4741 tgcgcggcag caatgcagga caaaacagca accattccgc acccaatcca cattttgctt
     4801 ttcaatgcga tgctccctgc gccgaagcgc tcgttgatgt tgtggccact cccagataat
     4861 tcccgtagcc cacaatcaca gtcgaaccca ccagcacaat cagcgtgcag gccaccagta
     4921 tgcgcgtgcg cagactcacg cccttccact cgcgcagcgc aatgccccac agcgtgctga
     4981 agatgatgat gctggccatg tgcagcgtcc agctggagaa cttaaagcgg cccatctgcg
     5041 tttcgcccat tgtgtagaag aagaactgca tgtaccacgt tgtcccggca agcgcggaaa
     5101 acagatagtt tgcgaggatg ggaacttttc cctgctgctc aactgccgtc atcgtgcgtg
     5161 tggccacttc cacgccagga gcatctatgg ccgtctcaat cacaggctct tcatttctgt
     5221 ccggaaaata ctcccaagtc tgtaacgcca agtattggta accggtacgg ttacgaatat
     5281 ggaggatgac gcaccacaca aaattggtcg tgaatcctcc agccagcagc accaccagca
     5341 caggcagccc ctgccacagc ggagaagtgc catgttgcac ggtaatggct ttgattggat
     5401 ctccagcggc aagaccatag gcaaagcagg cgctcatgat tccgcagaag gtcgctacca
     5461 gcagcccgcg tttcaggtcg aactcttcaa tcgccgcgcg cttctcttct tctgataatt
     5521 catgctcttt ggagatgccc gcggcaccgg caaatgcgat gcccgcaagg caaaccacca
     5581 cgccaatcag tatgatgatg cccgagcgcg tgcccagcac ttcagaagca aattgtccgc
     5641 gaaagattgg gggcatcagc gtgccgaagg cggccgtata ccccagcgcg acagccatcc
     5701 ccagcgacat gccgagatac cgcatcgtca gcccgaaggt caggccgcca aaaccccata
     5761 gcactccaaa aatgaatgtc cagacaatgc tgctcaaggg cgcctgatga agcacagcca
     5821 gcagatcacg cgtcatcaaa cc
//
LOCUS       DASRBV010000106        23120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_106172, whole genome shotgun sequence.
ACCESSION   DASRBV010000106 DASRBV010000000
VERSION     DASRBV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 23120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23120
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106172"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /gene="der"
                     /locus_tag="VFA02_04155"
     CDS             complement(<1..1417)
                     /gene="der"
                     /locus_tag="VFA02_04155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZU09071.1"
                     /translation="MKTPAQKSAPNAPLFAIVGRPNVGKSTLFNRITGSRRSIVGDEP
                     GITRDRIYGEVEWEGRIARVVDTGGIIPDEEALIPAEIFRQARVALEEAQAIVMVVDG
                     RTELASPDIDLARLLIRTGKPLFLAVNKIDTEALDASAENFRKLGIKNLVPVSAEHGH
                     NIGDLLDQVFAVLRFPELREETPDAAPEREEAIHRTHGKFQQQETRVAIIGRPNVGKS
                     TLLNALTGSSRAIVSPIAGTTRDAIDEVIEHAGQQLRIVDTAGIRRKGKTRLMAEKLS
                     VVMARKHLEAADVALLVIDATEGVTASDATIGGYAHESGRSVVIVVNKWDLVTTKRAD
                     GKPPAGRQIFEQQLRNVLKYLDYAPVVFISAVEGTNISRVLDTVIRVAKQRRHRISTS
                     QMNKFLERVDFERAGVPLAKKMRIYYMTQAAVAPPTFILFTNRDVKLHFSFERFLENQ
                     IRKAFGFEGSPIWFKVRAREKE"
     gene            1611..2516
                     /locus_tag="VFA02_04160"
     CDS             1611..2516
                     /locus_tag="VFA02_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09072.1"
                     /translation="MTSVVELDRISKSYENKIAVRELSLRIDSGTMFGLLGPNGAGKT
                     SSIRMMVGITMPDSGAVHLLGRPFARESLRRVGYLPEERGLYKKMKVIDQLIFMGELR
                     GLDPATAGNRAREWCERLQILEASEKKTEELSKGMQQKIQFIATLLHEPQLIIMDEPF
                     SGLDPVNASLLQDVLIELKKAGRAILFSTHRMDQVEKLCDSICVIHRGAAVLSGGMRE
                     IKSRYERNRVIIEFEGDNSFLQHPAIAEYKDYSGSVEIKLKPHADAQDLLHAAASKAK
                     IYKFELVEPSLEEIFIQTVGEKVDA"
     gene            2509..3753
                     /locus_tag="VFA02_04165"
     CDS             2509..3753
                     /locus_tag="VFA02_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09073.1"
                     /translation="MREIWLIAKREYLEQVRGKAFKITTILVPVLFAVIIGVSVLAGR
                     NSGSNKHIAVVATDAKVAEQIRQQLLADKDARMTVDLVTPASADDRQELIRQVDQKHL
                     DGFLWLDTGADGKTSAAYVARSSGDFMTESRLEDAVNHAVVQQRLAAHGIPTKDVDAI
                     TRTVKIKTEQVKNGKEVSSSAFGTFWAAYIMAFLLTFTLMMYGMNVGRSVIQEKTSRI
                     FEVMLSTVKPTHMLGGKLIGIGAVGLTQLAIWAAMGALFSGSALAATMMSGTVKIEIS
                     PQEIVLFALYFLLGYALYSTLFAGLAATCETEQELQQYTPLAAVPVWLSFSMIAFIVS
                     NPNSFWSVAISMFPPCAPIAMFLRIASQFPPLWQIAVSLALLAISVYIVVWFSSRIYR
                     VGILMYGKRATLPEMLRWLRYS"
     gene            3829..4830
                     /locus_tag="VFA02_04170"
     CDS             3829..4830
                     /locus_tag="VFA02_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU09074.1"
                     /translation="MSFAQERLFNLDGFHTRIATDVDAHAANLSGWNQQYDQLSSGKF
                     YGKINEIWMGRLQLFCEFTSHTVRQSCKPWQGSWWFGIPVHNFDLSRIGSYCIGKDAI
                     AVRPGSADFELLTPENFEILGVVVEQRELIEHAEMTEHVSLSPLCFNAFLLNTGQNKK
                     AQLQSLFQQILAEVKECPRLLEHPSSRQTIRSSILQALAEACSVQAQPIRPNRTEINH
                     HVLVLRIREYILEHQDEPVTISGICRHFFVSRRTLQNAFHSVTGMNPVAYLRAVRLNQ
                     VRRLLRDHNSGVSSVQDAAAQCGFWHLSQFACDYRKLFGELPSESLRRRSQFAERTG"
     gene            5136..8690
                     /locus_tag="VFA02_04175"
     CDS             5136..8690
                     /locus_tag="VFA02_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU09075.1"
                     /translation="MRTRCLFSRTELFFCIVLAAVSFFTGMASHAQSIQGSILGTVKD
                     SGGALVSGATVALLNLDDGSTRTTVSNSSGDYQFVDVKAGRYTVEIEQSGFQKWSATG
                     VVLAARQQLRVDAALEVGSVQQEVQVSGETISTIDTDTPSINAVYSSADVSNLPVNTR
                     ASASGTSALKIVGTLPGVQADHGDFSLQGGLPFQTEVSVDGVTVQSSTGNSPIADAFP
                     SSESISELRADGALNNAEFGQPGEITVTTKGGTNVIHGSAFWYHQNAAFNAIPYQYPK
                     ITQKPKLVANTYGASFGGPVVIPHLYNGHNKTFIYGAYEGWRHPSQSPYQFKVPSTLM
                     KQGDFSKYSSPGFSGLNNPFQGGSYGTRLPAINAAAQKLLSFYPDPNVGDPTAYVDDG
                     ISNYSVNKDSSGHSDQFDIRADQYFGSNQKFLLWGRFTWKNYPTNNPQPLTVPSSQYA
                     NQSRVLKISANWTITPHLINETGFGFTLFNTGQTNSFNGKTFTEGLGLQGLQNLFYNG
                     IPELDFNNVSPLNADRLTSITKSHTYVYTDSLTWVKSNHEFKFGADIRTLQAVTPLGF
                     NGSDNYGTFQFNTSNSTGMFTGVDFADFLLGLPNQTFYDVVQQDNDGRSVHYHFFAQD
                     QWKVNTDFTLSYGVRYELHPGYYDVGGDIGNFDPSLPLAGRSIYPDGKSKLLAQSFLA
                     SANACDPDGINFTNNSVVNGAPCMPVLTNSKAGYPAGLKKYPHLRFMPRFGFAWRPFH
                     DDKTAVRGGFGMYNITLLGANFYSLTGTLQAQTSQYTNTYNNTTHAIGYQWPVIYAGA
                     GNGGGCSNCYGTDYFGTANSTNWKDPYTEQWSLSVDHDFGSGYGVRLSYIGSETHQLV
                     WAPDENTLPFSNSVSANFQPLSARLFPNWGRINTRATGANESYHSLQAEFDHRFQRGL
                     QLNSSWTYAKALADNQGPANTGFAGESGGARATSILDRHADFGNVYGTRRHRWNTTAV
                     YDLPFGRGREFGSNMSRFADTVVGGWRLSSIFLWQTGPFESPYFPSGQGDPSGTGSGL
                     NGTQSGFDGGHRNQYPDQVAGTSYKPHHQTRFNWINPGAFTCPGYPNWQPGTACTTGS
                     GTGPSPNPIGRFGNAQPGSVVGPGTVNLSAGLSKTFSITERLKLRAEGTFTNVLNHTN
                     LSDPNMNISSPSFGLISSAISSDFGGARSGQVSMRLDF"
     gene            8795..10231
                     /gene="eat"
                     /locus_tag="VFA02_04180"
     CDS             8795..10231
                     /gene="eat"
                     /locus_tag="VFA02_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HZU09076.1"
                     /translation="MATEISGPQHGKVTHHELRRTLGPWQLWGIAVGLVISGEYFGWS
                     YGWASAGTLGFVVTSLLIAAMYTTFIFSFTELTTSIPHAGGPFAYARHAFGPFGGYLA
                     GAATLVEFVFAPPAIALAIGAYLSVQFPGLQPKHAAMGAYLVFMALNIIGVQIAAAFE
                     LFVTLLAILELLVFMCVVSPGFSIHNFMRGGWAGADHFSLHAIPGMFAAIPFAIWFFL
                     AIEGVAMAAEETKDPKRSIPIAYISGICTLVALAIGVMLFAGAAGDWTKLANINDPLP
                     QAMKIVVGPRSGWLHMLVWLGLFGLIASFHGIILGYSRQIFALARAGYLPRFLARVHP
                     RFKTPHVAILAGGAVGIAAIYSDQVITFGGQTLTANIVTMSVFGAILMYIISMMALFR
                     LRKTEPDAERPYRTPFYPLFPGFALLCAMICMLSMLYSNAKVAVLFVGLLFAGYIYFH
                     FTRHQRIVAPADDLLEKHEPLEPATGTE"
     gene            10244..11632
                     /locus_tag="VFA02_04185"
     CDS             10244..11632
                     /locus_tag="VFA02_04185"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HZU09077.1"
                     /translation="MAYSHIIGGKKYAFSDLKTLMAKASPARSGDSLAGIAATTEEER
                     MAAKMALADVPLTRFLNEALVPYEEDEVTRLILDSHDRAAFQPVQHMTVGEFRDWLLG
                     LDVDSAVLAQVAPGTTPEMAAAVSKLMRNQDLILAAQKCRVVTRFRNTIGLPGRLSVR
                     LQPNHPTDDVRGIAAAMLDGLLHGCGDAVIGINPATDSLPATVRLLRMMDEFRKRYEV
                     PTQSCVLTHVTSILHAIEMGAPVDLVFQSIAGTEKANASFGVHLALLREAQDAALSLH
                     RGTVGENVMYFETGQGSALSANAHQGVDQQTCEARAYAVARVFQPLLTNSVVGFIGPE
                     YLYNGKQIIRAGLEDHFCGKLLGVPMGCDICYTNHAEADQDDTDTLLTLLAVAGVNFI
                     MGVPGADDIMLNYQSTSFHDALYARRILHLKRAPEFEEWLQKMGITHANGDLNQAPSG
                     RLLEQATDLLKG"
     gene            11633..12367
                     /gene="eutC"
                     /locus_tag="VFA02_04190"
     CDS             11633..12367
                     /gene="eutC"
                     /locus_tag="VFA02_04190"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HZU09078.1"
                     /translation="MSENLWLQLRRFTHARIALGRAGDSLPTNRLLELGMAHAQARDA
                     VLKTINVEAVADQLKAAGFDSVSAHSAALNRAAYLGRPDLGRRLDEASRERLSNLHLS
                     RLPDVAFVIADGLSAVAAERHAVPVLRLTCATLAGWQIGPVIVAEQARVALGDEIGEL
                     LDAELLVMMLGERPGLSSPDSLGIYITYRPRVGKTDAERNCISNVRHKGLSYELAAHR
                     LHLLLNSARKLTMTGVGLKDVSELYL"
     gene            12578..13765
                     /locus_tag="VFA02_04195"
     CDS             12578..13765
                     /locus_tag="VFA02_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09079.1"
                     /translation="MSISDRLAARNQPQDIRKAIRLMPLHPDYRIQLANELFISDPDT
                     AQRALESAIRLDPFKASAWIKLGLLYEEKADLSGAEHALLNAAKVDKTYLPAWSLANF
                     YFRHDRTHEFWQWARTAAAIEPGDAQPLFRLAWYVEPDVDAIESHLQLRRSYMRVQFL
                     NFLMSQQETDAVGKASLHLLASDAGKNDTLPTLISACEWLLQYRRPDLALPLWNSLAE
                     QHLVPYPPISSGKENRITNAAFSRQPSSNGFDWHIGNAPGVSAFFNTSPNALGFEFSG
                     DEKEPVLLMYQVVPVTPGKAYKLTVDYSTSGISSGSGVAWVVSSFSSGAEIARTDSLS
                     AEQGGEVSACFVAPEDVKFINLLLEYQRQPGTVLVEGKITLYKTSLMATVNDACGTQK
                     VAQ"
     gene            14077..15033
                     /locus_tag="VFA02_04200"
     CDS             14077..15033
                     /locus_tag="VFA02_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU09080.1"
                     /translation="MLCVVAAGFIFAATALPQQQDHTPPSSIPYENDPGNLPAQPIGP
                     NDLLAVSVYDSPELTRTIRVNRDGEIRLPMLHDPIKVKGMYPAQVETAIAQALKDGKV
                     MVDPIVTVTIVEYQSRPINVVGAVKNPIVFQATHPITLLDAIARAGGISEDAGSEILV
                     RHEISQGDDPQWVTETVPVRQLIDQADQKLNITLHGGEQVLVPEAKKIYVVGNVRKPG
                     AFPVRNDENTTVLQALALSEGLMPFTAKEAYIYRHTSTGARQEVPIALDKIMKRKSPD
                     VALEANDILYIPDSKGKRLTAATIDRITGIGGQTAAYYVIWH"
     gene            15049..17262
                     /locus_tag="VFA02_04205"
     CDS             15049..17262
                     /locus_tag="VFA02_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU09081.1"
                     /translation="MRRQETISLQSRTVHSPEIDLVPAKIFASPGFGPPRVDATNRSY
                     LSQYLWIVKRHRWKLLAFIAFSVIATVIISKRLTPLYESTAVIDVDRQTPSAVLGDTT
                     TAAASAEDMQNFLATQERIIQSDAVLRPVVEKYHLMTKTLDKLKKDKILSSRLKDAPI
                     ELPGLKITQPPHTFLILISYRSPDPQLSADIANEIANSYIRHTYDIRFQAASQLTDYM
                     EKQLTELKAKMEQSASALAQFEKDLNVINPEAKTDVFSARLMQLNNDLTAAEADRASK
                     EAAYNAVKGGSMEAVEASEQGEQLRLLEGRLNEAQEKFAEVQTQFGPAHPAYKKAASQ
                     VKELQQQIDQLRDNIVQRVQTAYLQAVNKEALLQKDMLQAKAEFDQMNSKSFEYKALK
                     QEADTDKELYEQLIKKIREAGINASFESSSIRLADIARPALYPSFPKTKLNAVIALFG
                     STIIGLGIIFLMDAMNTVVFDSETLHRELHIPQLPSLPVGKFSPSTQPMQEFIEPGLT
                     KVETNGSGGIENSTFHEAIRTLRSSILLAGGLEQRPRSLLITSPSPAEGKSTVALYLA
                     VAHSQQQRKTLLIDGDLRRPANKLGLPRDRGLSDVVNGTLSWHEAVQTPEGYPDLDVL
                     SAGPASRLVADRVGIVMRSILEEAEREYDLVVIDSPPLLGFAEPLELASIVDSVVVIA
                     RAGRTTRSAIASAIGQLRQVHANIMGVVLNGIRADMSSKYYYYTTQYYSHYAKQD"
     gene            17452..18909
                     /locus_tag="VFA02_04210"
     CDS             17452..18909
                     /locus_tag="VFA02_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09082.1"
                     /translation="MLSRSRGKALVFYFRSLADSAYIELLKYIHFLHGCTETVLADEK
                     KQWVRINRNSLLRLFPAFVWGVIHDAAILLYWWIYLHWLLRNAGPVTERINDADLDLA
                     YLMPNPVDMGTTGGAISHIRGFLGGVRALGKKCRVFCGVPLAQESFRNEIVPPRGQRI
                     LFWEPPALAFNNTFIKGVRKQLENSHPKVFYQRHRRLSVAGAVLSRTTRVPLILEYNG
                     SEIWIADHWDPTSFRHWIVLCEEVSIRSASRILVVSEILKEELTRRGIPEERIVVNPN
                     GVDPEIFYPGCGGNKAREELEVYPEEILVAFVGTFSLWHGIEVLQNAIARILTEHPHI
                     RFKFVLIGEGILRDGMRMHLKKWESTRQVIFTGTLHHHKVAEYLDSADILVSPHIPMP
                     DGSPFFGSPTKLFEYMAMGKAIVASRLDQIAKVLEHEYTAWLVTPGSVNELVEAMLLL
                     ASNPDLRKQLGMAARLVAVQRHSWKHNAELALEGI"
     gene            18935..20245
                     /locus_tag="VFA02_04215"
     CDS             18935..20245
                     /locus_tag="VFA02_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU09083.1"
                     /translation="MRVLSQESSQVLRNVAETYSAQLFRIAGTLAFNIWFTRLLAPAD
                     RGSYGVAIAIALIGAQFGQTGLNTANTYFAAKKPHTSQALLGNSTLAAVTIGPVLVLV
                     TFLASKLFPNSIATTHNCFILAMCYIPFALGYFYFQGLLLGTHQIRTYNFLEIINRYV
                     PLVFLALLVWIGRVTSTTVLAGVLLGQIIACIWAWKSLYKTTDGVRISWPLMGDMFRY
                     GVKIHLATIFSFLLFRADLLMVARMRNAEEAGYYSLATSLAEYMSLPAFVVGTILLPH
                     LSALNEPARKFQMMLHWLGGIAIITIPLFLLAGYLGHPMIRWVCGAKYLPSFSGFLWL
                     LPGVYFLGLACVSVQFITSIGYKLSVSAAWFVALVLNLISNLYVIPHYGFVGASITSS
                     ACYGLAFLFIFLIGWKYRNSEESAPTITLAPIPPEESIPYIEPA"
     gene            20267..21511
                     /locus_tag="VFA02_04220"
     CDS             20267..21511
                     /locus_tag="VFA02_04220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09084.1"
                     /translation="MDYGLKNENIVCFAGEDWWYHHPHSKNHLLKRYSRKNKVLFVNS
                     ITMGLPSVSNPDFFLKIRRKLRSYMRWLKKAPEGLWVMTPVNLPFYGSRIGRWTNKVL
                     LVIQLRLAMALLNMRHPILWIAIPSAADMVGKLGEKAVLYQVSDKYDANEDSALSPRI
                     IREFDRELKSAADVVLYSGRKLFQEATEPNRYFLEQAVDFEHFSSLPDAIVEELEGVP
                     HPILGYFGAMDYVMDTELIEEVARLRPEWQWVFIGLKSNLIQITAPNVRFLGSKPYKD
                     LPKYIRYFDVCVLPWRHSNAFTSYGSAIKVREYLATGKPVVISPLYEYLEIPGLRIYR
                     GKDEFIRNVEDALAECGPKQSLARQSVVKNCTWDVRARELAELVQMVLDGKQYVFQNY
                     APWITNRAERIKRSPVMEGPEA"
     gene            21511..>23120
                     /locus_tag="VFA02_04225"
     CDS             21511..>23120
                     /locus_tag="VFA02_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU09085.1"
                     /translation="MKALLEDMKSGEVAMHEVPAPEPAHGCVLVRTAFSAISAGTERV
                     KIESGQKSLLGKAMARPDLVKQVIDSARANGIKTAWQKVQARLDTLSTLGYSCSGYVI
                     EVGEGVTEFHPGDRVACAGAGYANHCEINVVPKNLVARVPDNVDLDAAALTTIGAIAM
                     QGVRQANVTFGETIAVIGAGLVGILAIQILRAAGCRVIAIDLSPDRAKKAAALGAHLG
                     LSSVDAGMESAIYAFSRYGVDAAIITAATKSAEPLELAARLLRDRGRITIVGDVGMGV
                     SRGNMYMKELSLLMSRSYGPGRYDPEYEEEGHDYPIGYVRWTEKRNMEAFLDLLSCGT
                     LNVKPLLEHRYPVEKGGKAYSDLTTGIYTGIIDYHAPSGPTSFARPADAIQSPRPRAS
                     GMVRIGCLGAGSFARGIIFPHLRALKQVLFESVATNSGVAAESARKGFGFHVAETPSA
                     LLANPNVDMAFVLTRHDSHAFYVCSALEQGKAVFVEKPLATDLEQLDAVKATYERMIA
                     QGKHPFLMVGFNRRFAPFTEKVCNFFAGR"
BASE COUNT         5441 a         5789 c         6203 g         5687 t
ORIGIN      
        1 cttccttttc tctggcgcgc actttgaacc atattggaga accttcaaaa ccgaaggctt
       61 tgcgtatctg attttcgagg aagcgctcga aggaaaagtg cagctttacg tcccggtttg
      121 taaaaagaat gaatgttggc ggtgcgacgg cggcctgggt catgtagtag atgcgcattt
      181 tctttgccag agggaccccg gcgcgctcaa aatccacgcg ctcaagaaac ttgttcattt
      241 gacttgtgga aatgcggtga cgacgctgct ttgctacgcg aatgacggta tcgagcacac
      301 gcgagatatt ggtgccttca acagcagaga tgaaaaccac cggagcgtaa tcaagatatt
      361 taaggacatt ccttaactgc tgctcaaaga tttgccgccc ggccggaggc ttgccgtcgg
      421 cgcgcttcgt agtcacgagg tcccatttat tgacgacgat cacgaccgac cgcccactct
      481 catgcgcgta gccaccgatg gtcgcgtccg atgcggtcac gccctcagtt gcgtcaatga
      541 cgaggagggc cacatcggct gcttcaaggt gtttgcgcgc catcacaacg gagagctttt
      601 ccgccatcag gcgcgttttg cctttgcggc ggatccctgc cgtgtccaca atacgaagct
      661 gctggcctgc atgctcgatg acctcgtcta tggcatcgcg ggtggtgcct gcgatgggcg
      721 aaacaatggc gcgcgatgag ccggtgagcg cattgagtaa ggtggatttg ccgacattgg
      781 ggcgtccaat aatggcgacg cgcgtttcct gctgctgaaa tttgccgtgc gtgcggtgga
      841 tggcctcttc gcgttccggc gcggcgtcag gcgtttcttc ccgcagctca gggaacctga
      901 ggacggcaaa aacctgatcg agcaggtcac cgatattgtg cccgtgttca gcggagacag
      961 gaacgaggtt tttgattccc aatttgcgga agttttctgc cgaggcgtca agggcttccg
     1021 tatcaatttt gttgactgcg agaaagagcg gctttccggt tctgatcagc aggcgcgcga
     1081 gatctatgtc cggggatgcg agttcggtgc ggccatctac gaccatgacg atggcctggg
     1141 cctcttcgag agcaacgcgg gcctgacgga agatttctgc agggatgagg gcctcttcgt
     1201 cgggaatgat gccgcctgtg tccacgacgc gggcgatgcg gccctcccat tcgacttcgc
     1261 cgtagatgcg gtcgcgggta attcctggct catcgccaac gatggagcgg cgcgaaccgg
     1321 taatgcggtt gaaaagcgta gacttgccga cgttggggcg gcctacgatg gcaaaaagcg
     1381 gcgcattggg ggcggatttt tgtgcaggag tcttcaattt tcagaccaaa tgatgattca
     1441 gggcagagtt ggaagcattg gcaatgccag gccatgaaat taaatggggg cttcctgata
     1501 aattttaccg gaatgagaag tgcatccgga aaaaagcatt tattttgaac ctcttttcgc
     1561 tgttcttcgt atacactgac gccacatcct gatgggagaa atctgcctgc atgacttcgg
     1621 tcgttgagct ggaccgtatc tcaaaatcgt atgaaaacaa aattgctgtc agggagttga
     1681 gcctgcgcat tgactcaggg acgatgtttg gcctgcttgg accgaatggc gccggcaaga
     1741 cctccagcat tcgcatgatg gttggtatta cgatgccgga ttccggggcg gtgcatctgc
     1801 tgggcaggcc ctttgcccgg gagagtctgc gcagggtcgg ttatctgccg gaagagcgcg
     1861 gcctatacaa gaagatgaag gtcatagacc agctcatctt tatgggcgag ctcaggggac
     1921 tggatccggc gacagccgga aaccgcgcac gtgagtggtg cgaacgtctt cagatacttg
     1981 aagcgtcgga gaagaaaaca gaagagcttt ccaaaggcat gcagcagaag atccagttca
     2041 ttgcaacact gctgcatgag ccgcagctca tcattatgga cgagcctttt tcaggactgg
     2101 atccggtgaa cgcgtcgctt ttgcaagacg tgctgattga gttgaagaaa gcaggcaggg
     2161 ccatcctgtt ttccacgcac cgcatggacc aggtagaaaa gctctgtgat tccatctgcg
     2221 tgattcatcg cggagcagcc gtgctttccg gcggcatgcg cgagatcaaa tctcgctacg
     2281 aacggaaccg ggtcatcatt gagtttgagg gcgacaattc tttccttcag catcctgcga
     2341 ttgcggaata caaggactat tccggttccg tggagataaa gctgaagcct catgcagacg
     2401 cgcaagacct tctgcatgca gcggcgagca aagcaaaaat ttacaaattt gaactggtcg
     2461 agccctcctt agaggaaatc tttatccaga ccgtgggaga aaaagtggat gcgtgaaatc
     2521 tggctgattg caaaacgcga atatctcgaa caggtccgag ggaaggcctt caagattaca
     2581 accattcttg ttccggtgct gtttgcggtg attattggcg tttcggtcct tgcgggcagg
     2641 aattccggca gcaacaagca tattgctgtt gtagcgacag acgcaaaggt cgcggaacag
     2701 atccgccagc aattgctcgc agataaagat gcccgcatga cggtagatct ggtgacgcct
     2761 gcatccgcgg atgaccggca ggagcttata agacaggtag accagaagca tctggacggg
     2821 tttctatggc tggatacagg tgcagacggc aagaccagcg cggcctatgt tgcgcggtca
     2881 tccggagatt tcatgaccga gtcccggctt gaggacgcgg tcaaccacgc cgtggtccag
     2941 cagcgccttg cagcgcacgg catcccgaca aaagatgtag atgcaattac gcgaacggta
     3001 aaaatcaaga ccgagcaggt aaagaacggc aaagaggttt ccagcagcgc ctttgggacc
     3061 ttctgggcgg cctacatcat ggcatttctg ctgacgttca cgctgatgat gtatggcatg
     3121 aatgttggac ggtctgtcat tcaggagaag acctcgcgta tttttgaagt gatgctctcc
     3181 acggtgaagc caacgcatat gcttggcgga aagctcatcg gcattggcgc tgtcgggctg
     3241 actcaactcg cgatctgggc cgccatggga gcgctgtttt ccggtagcgc gctggctgca
     3301 accatgatgt ctggcacggt aaagatagaa atttcaccac aggaaattgt cctgttcgcg
     3361 ctttatttcc tgctgggata tgcgctctac agcacactgt ttgccggact ggcagcgacc
     3421 tgcgaaaccg agcaggagtt gcaacaatat actcctcttg ccgcggtccc tgtgtggctg
     3481 agcttcagca tgattgcctt tatcgtgagc aacccgaatt ccttctggtc ggtggccatc
     3541 tccatgttcc cgccatgcgc gcccattgcc atgtttctgc gcattgcgtc ccaattccct
     3601 cccctgtggc agattgcggt ttctctggcg ctgctggcaa tctccgttta catcgttgtc
     3661 tggttctcgt cgcgcattta tcgcgttggc attttgatgt atggcaagcg cgctactctt
     3721 ccggagatgc tgcggtggtt gcgatatagt taaccccctt ggacgaggag aagaagtcac
     3781 aatctgttag cactcttgcg ataaaatccc tgacacaggt atcgcaagtt gagttttgca
     3841 caagaaagac tgttcaacct cgatggattt catacccgca ttgccacaga cgtggatgcc
     3901 catgctgcaa acctgtccgg atggaaccag caatatgacc agctttcatc cggaaagttc
     3961 tatggcaaaa tcaatgagat ctggatgggc aggttacagc tgttttgcga atttaccagc
     4021 catactgtcc ggcagtcctg caagccgtgg cagggttcct ggtggtttgg aattcccgtt
     4081 cacaacttcg atctgagcag gattggcagc tactgcatcg ggaaagatgc catcgctgtg
     4141 cgtcctggaa gcgcagattt tgaactgctg acacccgaga actttgagat cctgggcgtt
     4201 gttgttgagc agcgtgaact gatagagcat gctgagatga cggagcacgt atcgctttct
     4261 ccgctttgtt tcaatgcatt tttgctcaac accggacaga acaaaaaggc ccagcttcag
     4321 tcattgtttc aacagatatt ggccgaggtg aaggaatgtc cccgcctgct ggaacatccg
     4381 tcttcgcgac agactatccg gtcttcgatt ctgcaggcgc tggcagaagc atgcagtgta
     4441 caggcgcagc caatccggcc taacagaacg gagatcaatc accatgttct ggttttgcga
     4501 attcgcgaat acattctgga acaccaggac gagcctgtga ccatatccgg catctgccgg
     4561 catttctttg tgagccgtcg aacgctgcag aatgccttcc acagcgtgac cggaatgaat
     4621 cctgtcgcat atcttcgcgc agtccgcctg aaccaggtgc gccgcttgct gcgcgaccac
     4681 aactctggag tcagctctgt gcaggatgcg gcagcacaat gcgggttctg gcatttgagc
     4741 cagtttgcct gtgattatcg aaagctcttt ggagaattac cttctgagtc gttacgccgg
     4801 cgttcacagt ttgcggaaag aaccggctga agaacgactg ctggctccag caaatactgg
     4861 gatgaaacgc tggaattctt cgtcctcctg ggatgcgttg cgtaagctga gattcatcaa
     4921 aattcacgag cgcagttctg tacaccggcc cataggaaaa gcgattcagc gagatttttt
     4981 attctctttt cttatgcttc ccataaggtt ttcgttgaat ttcatgtaaa catctgcgtt
     5041 tttgccgatt tttgataacg gcatcactcc cgctgcgcct attcttcccg tacacgatcc
     5101 cctgttgcta aagcattttt tcaggaggca ttctcatgcg aacgcgttgt ctgttttcgc
     5161 ggacagagtt gtttttttgc attgttctgg cggccgtcag tttttttaca ggcatggctt
     5221 ctcatgcgca atccattcag ggaagcattt tgggcacagt aaaagattca ggcggtgcgc
     5281 tggtgtctgg cgctacggtg gcccttctca atcttgatga tggttcgacg cgcacgactg
     5341 tttccaacag ttccggtgat tatcagtttg tggatgtgaa ggccggacgc tataccgttg
     5401 agatagagca aagcggtttt cagaagtggt cggccaccgg cgttgtgctg gctgcgcgac
     5461 agcagttgcg cgtagacgct gcgctggaag tgggaagtgt ccagcaggag gtccaggttt
     5521 caggagaaac catcagcacc attgatacgg acacaccatc tattaatgcc gtctattcct
     5581 ctgccgatgt ctcgaatctt ccggtaaaca cgcgcgccag cgcgagcggc acaagcgccc
     5641 ttaaaattgt gggcacgctg cccggggtgc aggcagacca cggcgatttc tctctgcagg
     5701 gtgggctgcc gtttcagacg gaagtctctg ttgatggcgt cacggtccaa agctcaacag
     5761 gaaattcacc cattgcagat gcttttcctt cgagcgaatc tatctcagag ttgcgcgccg
     5821 atggagcgct gaacaatgcc gaatttggcc agccgggcga gattacggtc acgaccaagg
     5881 gtggaaccaa tgtcatccac gggtcagcgt tctggtatca ccagaatgcc gcctttaatg
     5941 ctattccata tcagtatccg aagatcacgc agaagccaaa gctggttgca aacacttatg
     6001 gagcaagttt cggcggcccg gttgtcattc cacatctgta caacgggcac aacaaaactt
     6061 ttatctacgg cgcctacgaa ggctggcgtc atccatcaca aagcccttat cagttcaaag
     6121 tgccgagcac gttgatgaag cagggagatt tctcaaaata ctcttcaccc ggctttagcg
     6181 ggctgaataa tccttttcag ggcgggagct atgggaccag gctgcctgcg atcaatgcag
     6241 cggcgcaaaa gctactctcg ttttatcctg atcccaacgt tggcgatcct acggcatatg
     6301 tagatgatgg gatatcgaac tatagcgtaa acaaggattc aagcgggcac tctgaccaat
     6361 ttgatattcg ggcagaccag tattttggct caaaccagaa gttcctgctc tgggggcggt
     6421 tcacgtggaa gaactatcct acgaacaatc ctcagcctct tacagtgcct tcttcgcaat
     6481 atgctaatca gtcgcgtgta ctcaagatca gtgctaactg gaccattaca ccgcacctga
     6541 tcaatgaaac tggttttggc tttaccctct tcaacaccgg acagacaaac agctttaacg
     6601 gcaagacatt tacagaaggg ctgggactgc aggggctgca gaatcttttc tacaatggaa
     6661 tccctgaact tgacttcaat aatgtcagcc cgctgaacgc ggaccgcctg acttccatca
     6721 ccaagtccca cacctacgtc tataccgatt cactgacatg ggtaaaaagc aatcatgagt
     6781 tcaagtttgg cgcggatatc cggacgctgc aggcagtgac accgctgggc tttaatggtt
     6841 ctgataatta cggcacgttc cagttcaaca cgtcaaacag cacgggaatg ttcacgggag
     6901 tagattttgc cgatttcctg ctgggcctgc ccaaccagac tttctatgac gttgtgcagc
     6961 aggacaatga cgggcgctct gtccattatc atttcttcgc gcaggaccaa tggaaggtca
     7021 ataccgattt cacactcagc tatggcgtgc gctatgagct gcatcctggt tattacgatg
     7081 tgggcggaga cattggaaac tttgatccga gtctgccgct ggccggacgc tcaatttatc
     7141 cggatggcaa gtccaaactg ctcgcacaga gttttctggc aagcgcgaat gcgtgcgatc
     7201 cggacggcat caactttacc aacaattcgg ttgtcaatgg cgctccctgc atgccggtgc
     7261 tgacaaacag taaggcaggt tatccggctg gactgaagaa atatccgcac ctgcgcttta
     7321 tgccgcgctt tgggtttgca tggcgtccct tccatgacga caagacagca gtccgcggcg
     7381 gattcggcat gtacaacatt acgctgctgg gcgcgaactt ctactcgctt accgggacat
     7441 tgcaggcgca gacgtcgcag tacacaaaca cttacaacaa caccacacat gcgatcggct
     7501 atcaatggcc ggtgatctat gcaggcgccg gcaatggcgg cggatgctcc aactgctatg
     7561 ggaccgacta ctttggaaca gccaacagca cgaactggaa agatccatac acggaacaat
     7621 ggtcgctcag cgtagaccat gactttggtt cgggatatgg ggtgcggttg tcctacattg
     7681 gctctgaaac acaccaactg gtatgggcac ctgatgaaaa tactctgcca ttctccaatt
     7741 cagtctcggc aaacttccaa ccgttgagcg cccgtctctt tccgaactgg ggacgcatca
     7801 acacccgcgc aacgggagcc aatgaaagct accactcttt gcaagcggag tttgaccacc
     7861 gctttcaaag aggactgcaa ctgaactcgt catggacgta tgccaaggcc ctggcagata
     7921 atcaaggccc tgccaacacc ggctttgcag gagaaagcgg cggcgcgcgc gctacgtcca
     7981 tccttgaccg tcatgctgat ttcggcaacg tctacggcac acgccgtcac cgctggaaca
     8041 caacggcggt ctacgatctc ccttttggac gcggacgtga atttggaagc aatatgtcca
     8101 gatttgccga tactgttgtg ggcggatggc gtttgtcttc gatcttcctg tggcagacag
     8161 gcccatttga gtcaccttat ttcccgagcg ggcagggtga tccttcggga acaggttccg
     8221 gcctcaatgg aacacaatcc ggttttgatg gcggccatcg caaccagtac cctgatcagg
     8281 tagctggaac cagttataag ccacatcatc agacacggtt taactggatc aatccgggtg
     8341 cgtttacctg ccctggttat cccaactggc agcctggaac agcctgcacg acaggcagcg
     8401 ggaccgggcc atcaccaaat ccgattggcc gttttggaaa cgcgcagcct ggctctgtag
     8461 ttggccccgg aactgtgaac ctgtctgccg gactgagcaa gaccttctcc attactgagc
     8521 gtctgaagct gagagcggaa ggcacattca ccaacgtgct taaccatacg aacctgagcg
     8581 atccgaacat gaacatcagt tcaccgagct ttggattgat ttccagcgca atcagttctg
     8641 atttcggagg agcgcggtcg gggcaggttt cgatgcgcct ggacttttag caaaggcgct
     8701 tgcctgatgt aaggtaatgt cattctggcg gggcgtagaa ggcagcaggt tcttcgctcc
     8761 gctccgaatg acgcgttctt attgatcaaa aaaaatggcg acagagatat ccggtccgca
     8821 acacggcaaa gtcactcatc atgagttgag gcgaacgctg ggtccgtggc agctttgggg
     8881 cattgctgtg gggctggtca tctctggtga atattttggc tggagctatg gatgggcgtc
     8941 ggcaggcacg ctgggattcg ttgttacgtc attgctgatt gcagccatgt ataccacctt
     9001 tatcttcagc tttactgaac ttacgacttc gattcctcac gcaggcggcc cttttgccta
     9061 tgcgcgccac gcttttgggc cgttcggcgg atatcttgcg ggagcagcta cgctcgtgga
     9121 gtttgtgttt gctccgcccg cgattgccct tgcgattggc gcgtatttaa gcgtgcagtt
     9181 tccgggattg cagcctaagc acgcggccat gggcgcgtat ctggtgttca tggcgctgaa
     9241 tatcattgga gtgcagattg ccgccgcttt cgagctgttt gtaacgctgc tcgctattct
     9301 tgaacttctt gtgtttatgt gtgtggtctc gccggggttt tccattcaca acttcatgcg
     9361 cggtggatgg gccggagccg accatttcag ccttcatgcg attccgggca tgtttgccgc
     9421 gattccgttt gcgatctggt tcttcctcgc cattgaaggc gtcgccatgg cggcggaaga
     9481 aacgaaggac ccgaaacgtt caataccgat tgcgtatatc agtggaatct gcactctggt
     9541 ggcgcttgcg attggagtga tgctctttgc gggcgccgcc ggagactgga cgaaacttgc
     9601 gaacatcaac gatccactgc cacaggccat gaagatcgtc gttggcccaa ggagcggatg
     9661 gctgcacatg ctggtctggc ttggtctgtt tggcctgatt gcctcgtttc atggaatcat
     9721 tctcggctat tcgcgacaga tttttgccct ggcgcgcgcg ggttatctgc cgcggtttct
     9781 tgcgcgggtg catccgcgat ttaagacgcc gcatgtcgcc attcttgccg gaggcgcggt
     9841 cggcattgct gctatctaca gtgaccaggt cattacattt ggcggacaga cattgacggc
     9901 gaatattgtc acgatgtcag tgtttggagc gatcctgatg tatatcatca gcatgatggc
     9961 gctctttcgc ttgcgtaaaa cagaaccgga tgctgaacgc ccctatagaa caccttttta
    10021 cccgctgttt ccgggctttg cactgctatg cgccatgatt tgcatgttga gcatgttgta
    10081 tagcaatgct aaagttgcag ttctttttgt ggggcttctg tttgccggat acatttactt
    10141 tcatttcacc cggcaccaaa ggattgtcgc gccagcggat gatctgcttg aaaaacacga
    10201 acctctggaa ccagcaaccg ggacggagtg agcggagtaa gggatggcgt attcacatat
    10261 catcggaggg aaaaaatacg ctttcagcga tctgaaaact ctgatggcaa aagcaagccc
    10321 cgcgcgctcc ggggattcgc ttgccggcat tgctgcaact acagaagaag agcgcatggc
    10381 cgcgaagatg gcgcttgccg atgttccatt gactcgcttt ctcaatgaag cgctcgttcc
    10441 ctacgaggaa gatgaggtaa ccaggctgat actggacagc catgaccgcg cagcattcca
    10501 gcctgttcag cacatgacgg tgggcgagtt tcgagactgg cttctgggtc tcgatgtaga
    10561 cagcgcggtg cttgctcagg ttgcgcccgg aaccactccg gagatggcgg ctgctgtatc
    10621 gaaactgatg cgcaatcagg acctgatact tgcagcgcag aagtgccgcg ttgtgactcg
    10681 gtttcgcaat acgattggtc tacccggacg gctctcagta cgcctgcagc cgaatcatcc
    10741 gacagacgat gtaaggggca ttgcggcggc catgctcgat gggcttctac atggatgcgg
    10801 cgatgctgtg atcggaatca atcctgcgac agacagtctt cctgcgactg tccggctgct
    10861 caggatgatg gacgagttcc gcaaacggta tgaagtgcct acacaatcct gtgtgcttac
    10921 gcatgtgacg agcattcttc atgccattga aatgggagca ccggtcgatc ttgttttcca
    10981 gtccattgcc gggacagaaa aagcgaatgc cagcttcggg gtgcatcttg cgcttctgcg
    11041 cgaagcacag gatgcggcct tgtcactgca tcgcggcacg gttggcgaga acgttatgta
    11101 ttttgagaca gggcaaggca gcgcgctttc cgcaaacgca caccagggtg tagaccagca
    11161 gacctgtgaa gctcgcgcct atgctgttgc ccgcgtcttc cagcccttgc ttacgaactc
    11221 cgtggtcggt tttatcggcc cggagtatct gtataacgga aagcagatta tccgcgccgg
    11281 acttgaagac catttctgcg gcaagctgct gggtgttccc atgggttgtg acatctgtta
    11341 tacgaaccat gcagaagccg accaggatga tacggacaca ctgctgacgt tgctggctgt
    11401 agcgggagtc aacttcatca tgggtgttcc cggtgctgac gacattatgc tgaattacca
    11461 gagcacctca tttcatgacg ctttgtatgc caggcggatt ctgcatttga aacgcgcgcc
    11521 tgagtttgaa gagtggctcc agaagatggg gatcacgcac gcgaacggcg atctgaacca
    11581 ggcgccttcg ggacggttgc tggagcaggc cacggatttg ctgaaaggct gaatgagcga
    11641 gaatctgtgg ttgcaactca ggcgatttac acacgcgcgc attgctctcg gtcgcgctgg
    11701 agacagcctg cccacaaaca gattgctcga acttggcatg gcgcacgcgc aggcgcgcga
    11761 tgcagtgctg aaaacgatca acgtggaagc cgtcgccgat cagctcaaag ctgcgggctt
    11821 tgattctgtt tctgcgcata gcgcagcact gaaccgcgcg gcctatctgg gccgcccgga
    11881 ccttggccgc aggctggatg aggccagccg tgagcgcctg tctaacttac acctgtctcg
    11941 acttccggac gttgccttcg tcatcgctga tggcctttct gcagttgcag cggaacgcca
    12001 cgcagtgcct gtcctgcgcc ttacctgtgc cacgctcgcc ggatggcaga ttgggccagt
    12061 gattgttgcg gaacaggcac gcgtcgcact tggggatgag atcggcgagc tactggatgc
    12121 tgagctgctg gtcatgatgc tgggcgagcg tcctggcctg agttcgcctg acagcctggg
    12181 catctatatc acatacaggc ctcgagtggg caagacggac gcggagcgta actgcatctc
    12241 caatgtgcgc cataaaggac tctcatatga gcttgctgcg cataggctgc atctgcttct
    12301 caacagcgcc agaaaactta caatgactgg cgttggtctt aaggatgtaa gcgagctcta
    12361 cttgtgatac ttgcgatagc gcccttggaa cgtttctgat tcagatatag tcctgtcgcg
    12421 ctgctgcaat agagctgccg cacttttgtt ctaactgaat ccaggggtat tattttccgg
    12481 aagataaatc tcttccagtt ttatgcgcgc aggcacattg gcccttaagt ccgggtgttt
    12541 tgtcgtattc accgcaggca ttatctggaa taccggtatg agtatttcgg accggctggc
    12601 ggcacgcaac cagcctcagg acattcgcaa agcaatccgc ctgatgccac tccatccgga
    12661 ctatcggata caactggcca atgagctttt tatttccgat cctgatacgg cgcagagagc
    12721 gctggaaagc gcaatccggc tggatccgtt taaagccagc gcatggatca agcttggcct
    12781 tctctatgaa gagaaggccg atttgtccgg ggcggaacat gctcttctga acgctgcaaa
    12841 ggtggacaag acgtacctgc cagcatggtc tttggcgaat ttttacttcc gccacgacag
    12901 gacacatgaa ttctggcagt gggcgagaac ggcggcggcc atagaaccag gtgacgcgca
    12961 gccgctattc cgtctggcct ggtatgtgga accggatgta gatgcgattg aatcgcatct
    13021 gcagttacgg cggtcatata tgcgggtaca attcctcaac tttctcatgt cgcagcagga
    13081 aacagatgca gtggggaaag cttctcttca tttactggct tcagatgctg ggaaaaatga
    13141 cactttgccg acgctcatct ccgcatgtga atggctgctt caatacaggc ggcctgacct
    13201 tgcattgccg ttatggaaca gtctggcgga acagcatctg gtgccgtatc ctcccatttc
    13261 ttcaggcaag gaaaacagga ttacaaatgc cgccttctca agacagccca gctcaaacgg
    13321 atttgattgg catattggga acgcaccagg ggtttcggct ttctttaaca caagtccaaa
    13381 tgcattgggg tttgaatttt ccggggacga gaaagagccc gttttactta tgtatcaggt
    13441 tgtcccagtc acgcccggaa aagcctacaa actgacagtt gattactcaa catcaggcat
    13501 ttccagcggt agcggtgtgg cctgggtggt aagcagtttt tcttcgggcg cagagattgc
    13561 ccggactgac agtctttccg cagaacaagg cggagaggtc tcagcatgtt ttgttgctcc
    13621 agaggacgta aagtttatca atcttctgct ggaatatcag agacagccgg gtactgtgct
    13681 cgtcgaaggc aagataactc tttataagac gagtttgatg gcgactgtaa atgatgcctg
    13741 tggtacgcag aaagttgccc agtgaagata tttttgattt agtacaaagc tgagatgaca
    13801 agggccagcc tcaaagcgta taaagatagg ctcagtcgaa gctcacgatt gaagagcagc
    13861 taagtgtgtc cacaattagt taattggcgg tatgtgttcc ataccaacaa ggcagtaccg
    13921 gaaagacgat cctgtagtta ggggagtatt atggattgcc atgctgttca atatccagca
    13981 aaggcttttc gttgtgcatt ttgcacgata aggagttctt cgggcagcgg gtctggaagc
    14041 gggtgcccaa cctcaaccag gcgcgcaata cgacccttgc tttgtgtagt cgctgcggga
    14101 tttatttttg ctgccaccgc ccttccgcag caacaggacc atacaccacc ttcgtccata
    14161 ccctatgaaa atgatcctgg aaatttacct gcccagccca ttggccccaa tgacctgttg
    14221 gccgtgtcgg tttacgactc cccggagctt acgcgcacaa tacgtgtgaa ccgggatgga
    14281 gagatccggc tgccgatgct ccatgacccg atcaaggtca agggtatgta tccagcgcag
    14341 gttgaaactg cgattgccca ggcattaaag gatgggaagg tgatggtgga tcccatcgtc
    14401 actgtcacta ttgttgaata ccaaagcagg cccattaatg ttgttggcgc ggtaaagaac
    14461 ccgatcgtgt ttcaggcaac gcatccgatt actttgctgg atgctatcgc acgcgccggc
    14521 ggcatcagcg aagatgcggg ctcagagatt cttgtaagac atgaaatttc ccagggagat
    14581 gatccacagt gggtaacaga gaccgtgccg gttcgccagc tgattgatca ggccgaccag
    14641 aaattgaaca tcaccctgca tggcggagag caagtactgg ttcccgaggc aaaaaaaatc
    14701 tatgtggtag gcaacgtaag aaagcccggc gcattccctg tacgcaatga tgagaacacc
    14761 actgttctgc aggcgctcgc gctctcagag ggactgatgc cttttaccgc gaaagaagct
    14821 tatatctacc ggcacacgtc taccggagcc aggcaggaag ttcccattgc actggacaag
    14881 atcatgaagc gcaaatcgcc ggatgtagca ttggaagcca atgacattct ttacattccc
    14941 gacagcaaag gaaaacgact gactgccgcg accattgaca gaattaccgg tattggaggg
    15001 cagactgcag cttattacgt tatctggcac taaaaggggc ccaatttcat gcgcaggcaa
    15061 gaaacaattt ccttacaaag caggaccgtc cattcaccag agatagacct ggttcctgct
    15121 aagatttttg cttctcccgg cttcggcccg ccacgggtag acgcaacgaa ccggtcttat
    15181 ctttcgcaat acctttggat tgttaagcgc catcgctgga aactgctggc ctttattgct
    15241 ttttcagtga ttgcaacagt cattatttcc aagcgcctta cgcccctcta tgaatctaca
    15301 gctgtaattg atgtagaccg gcagacacca tcggcggtcc tgggagatac aactacagcc
    15361 gcagcttcag cggaagatat gcagaacttt ctggctaccc aggaaaggat tattcagtcg
    15421 gatgcagttc tgcgcccggt ggtggagaaa taccacctga tgaccaaaac actcgacaag
    15481 ctgaagaaag acaagatact ttcctcgcga ctcaaggatg ctcctatcga actgccgggc
    15541 ttaaaaatta cgcagccacc acatacattc cttattctga taagctaccg ctcgcctgac
    15601 ccccagcttt ctgccgatat agcaaatgag attgcaaaca gttatatacg gcatacgtat
    15661 gacatccgct ttcaggctgc ttctcaactg acggattaca tggaaaagca gctgaccgag
    15721 ttaaaggcga agatggagca atcggcgagt gccctggccc agtttgaaaa agacctgaac
    15781 gtcatcaatc ctgaagcaaa gacggatgtg ttttccgcgc ggttgatgca gctaaataac
    15841 gatctgaccg cggccgaagc tgacagggcc agtaaagagg ctgcatataa cgcagtaaag
    15901 ggcgggtcta tggaggcagt cgaggcttca gagcagggag aacaattacg cctgctggaa
    15961 ggaaggttga acgaggccca ggaaaagttt gccgaagtcc agacacaatt tggacctgcg
    16021 caccctgcat ataaaaaagc agccagccag gtaaaagaac tacaacagca gatagaccag
    16081 ttgcgcgata acatcgttca aagagtccag accgcctacc tacaggccgt taacaaagaa
    16141 gcgcttctgc agaaagacat gctgcaggcc aaggccgagt tcgaccagat gaactcgaaa
    16201 tcttttgaat ataaggcact caaacaagaa gcggacacag acaaggaact ttatgaacag
    16261 ctgattaaga agattcgcga agcaggaatt aatgccagct tcgaaagtag ttctattcgg
    16321 cttgcagata tcgcgcgacc cgctctttat ccttcattcc ctaaaacaaa actcaatgca
    16381 gtcatcgcgc tatttgggtc taccattatc gggcttggca ttatctttct gatggacgca
    16441 atgaatacgg tggtgtttga ttccgagacg ctacaccggg agctccatat tccacagttg
    16501 ccatcgctgc cagtggggaa gttttctcct tccactcagc ctatgcagga atttatagag
    16561 ccagggttga cgaaggtgga aacaaacggc tctggcggga ttgaaaacag caccttccat
    16621 gaagccattc gcacgctgag aagctccatt ctacttgcgg gaggactgga gcagcgccca
    16681 cggagtctac tcatcacgtc cccgtctcca gcagaaggaa aatccactgt ggctttatat
    16741 ctcgctgtag cgcatagtca gcagcagcga aaaacacttc tgattgatgg agacttacgc
    16801 cgcccggcca acaaattggg actgccacgg gaccgcggat tatctgatgt cgtgaatggt
    16861 acattatcgt ggcatgaagc cgtccagaca cctgaaggat atccagacct ggatgttctt
    16921 tcggctggac ctgcctcccg tttagtagcc gaccgggtag gaattgtgat gcgttccatt
    16981 ctggaagagg cagagcgcga gtacgatctg gtcgttattg attcgccgcc tctgctggga
    17041 tttgcagagc cgttggaatt ggcttccatt gtggacagcg ttgtggtcat cgccagagca
    17101 ggacggacaa cccggagtgc cattgcgagt gcaatcggtc agctacgcca ggtccatgcc
    17161 aacattatgg gggttgtatt gaatggaata cgggcagata tgagctccaa atattactac
    17221 tatacgaccc aatactatag ccactatgct aaacaggatt aagttgcagg ctacaatact
    17281 acaacaacta tagggtagcg aatggcaagc agtcagacag tatatctttg tgtctggaca
    17341 ggaaacacgg aaactgccag agacctggta agcagttact atccaggcgc tgacattata
    17401 gagttttctc atctgaggtt tcggagcagc agcttcaaag agcggcttca aatgctttcc
    17461 cgttctcgtg ggaaagcatt ggtattttac tttcgctccc tggctgattc ggcctatatt
    17521 gaacttttaa aatatatcca ttttctgcac ggctgcactg aaaccgtcct ggccgatgaa
    17581 aaaaaacaat gggtccgtat caatcggaat tccctactta ggctcttccc tgctttcgtt
    17641 tggggcgtga ttcatgatgc tgcgattctg ctctattggt ggatctattt gcattggcta
    17701 ctacggaatg cagggcccgt cacggaacgc ataaacgatg cagacctgga cttggcatat
    17761 cttatgccca accccgttga tatgggaact accggtggcg ctataagtca tattcggggc
    17821 tttttaggcg gagtgcgtgc gttaggaaag aaatgccggg tcttttgtgg agtacccctg
    17881 gcccaagagt ccttcaggaa tgagattgtc cctccacggg gacagcgaat tttattttgg
    17941 gaaccgccag cgcttgcttt taacaacact tttatcaagg gcgtccgtaa gcagttagag
    18001 aactcgcacc ctaaggtttt ttaccagcgc cacagacgat tatccgtagc aggcgcggtc
    18061 ctctccagga ccacacgcgt gcctctcatt cttgaataca atggttcgga aatatggatc
    18121 gcagaccatt gggacccaac atcttttcgc cactggattg ttctttgcga agaagtctcg
    18181 atacgttctg catcgcggat tcttgtcgtc tcagaaatat tgaaggaaga gttaacaagg
    18241 cgcggaattc cggaagagag aatcgtcgtc aatccgaatg gagttgatcc ggagattttt
    18301 tacccaggct gcggcgggaa caaagctcgc gaagaattag aagtctatcc cgaggaaatt
    18361 ttagttgcct ttgtcggaac attcagcttg tggcacggca ttgaggtact tcagaatgca
    18421 attgcccgaa tattaactga gcatccgcac atcaggttta aatttgtgct gattggtgaa
    18481 ggtattctgc gggatggaat gagaatgcat ttaaaaaaat gggagagcac ccgacaagtc
    18541 atttttaccg ggactttgca ccatcacaaa gttgctgagt atctagattc tgctgacata
    18601 ttggtttcac cccacattcc catgccggat ggaagcccat tttttggatc accgacaaag
    18661 ctttttgaat atatggcaat ggggaaagca attgtggcaa gcaggctgga ccagattgca
    18721 aaggtcctgg agcatgaata tacagcatgg ttagtaactc caggcagtgt gaatgagttg
    18781 gtagaagcta tgctgctact ggcatctaac cctgatctac gtaaacaatt aggaatggca
    18841 gcgcgtttgg tcgccgtaca acggcattct tggaaacata atgcagagct ggcactggaa
    18901 ggcatatagt ccggtattgt gagacttatt tttcatgcgt gttttgagcc aggaatcgtc
    18961 acaggtactc cgcaatgttg cggaaacata ttctgcacag cttttccgca ttgcagggac
    19021 cctggctttc aatatctggt ttacacggct cctggcccct gctgatcgtg gttcttacgg
    19081 agtagccatc gccatagcat tgatcggggc acaatttggc cagacagggc tgaatactgc
    19141 aaacacttat tttgccgcaa agaaacccca tacgagccag gctctattgg gaaacagcac
    19201 tcttgccgct gttacgattg gaccagttct ggttcttgtg acctttcttg cctcaaaact
    19261 ctttccaaat tccattgcta ctacccacaa ctgcttcatt ctggccatgt gctatatccc
    19321 gtttgcatta ggctatttct attttcaagg gcttctgttg ggaacacatc agatcagaac
    19381 atataacttt ttagagatta tcaatcgtta tgttccactt gtttttctgg cactgcttgt
    19441 ctggataggc agagtgacat ccacaactgt gctggccgga gtgcttcttg gccagataat
    19501 tgcctgtatc tgggcatgga agagcctgta taagactaca gatggagtac gtatttcgtg
    19561 gcccttgatg ggtgatatgt tccgctacgg agtgaagatc catctggcga ctatattttc
    19621 gtttttgctc ttccgggctg atctgctcat ggtggcgcgc atgaggaatg ctgaagaggc
    19681 tggatattac tctctggcaa catctttagc tgagtacatg agccttccgg cctttgttgt
    19741 aggcacaatc ctgcttcctc atttgtccgc gctcaatgaa cctgcaagaa agtttcagat
    19801 gatgttgcat tggcttgggg gcatcgccat catcacaatt cctttgtttc ttcttgcggg
    19861 atatttgggg cacccaatga ttcgatgggt atgcggtgcg aaatatcttc catcattttc
    19921 tggttttctg tggttgctgc ctggtgttta ctttttaggc ctggcatgtg tttctgtaca
    19981 gttcataaca agcattggat acaaattgag tgtatctgcc gcatggtttg tggcgcttgt
    20041 attaaattta atcagtaatt tgtacgtaat tcctcattat ggatttgtgg gggcatctat
    20101 cacatcgagt gcctgttacg gactggcgtt tctcttcatt tttctgattg gctggaaata
    20161 caggaactcc gaagaaagcg cacctactat aacactggct cctattccac cggaggagag
    20221 catcccgtat atagagcctg catagaccgg agaaccgcag aaacatatgg attacggctt
    20281 aaaaaatgag aacattgttt gctttgccgg tgaggactgg tggtatcacc atccacattc
    20341 caaaaaccac ctgctcaaac gctactcccg caagaacaaa gtgctctttg tgaactcaat
    20401 tacaatgggc ctgccctctg tttccaatcc tgacttcttt ttgaagataa ggcgaaagct
    20461 gcgcagctat atgcgatggc ttaaaaaagc cccggaaggt ttgtgggtga tgacgccggt
    20521 caatctgccc ttttatggct cacgcatcgg gcgctggacc aacaaagtat tgctggtcat
    20581 ccaattgcga ttggccatgg ccctgctcaa tatgcgtcac ccaattctgt ggatcgccat
    20641 tccttctgca gctgatatgg ttggaaaact tggcgagaaa gccgtccttt atcaagtatc
    20701 agacaaatat gatgcgaacg aggacagtgc tttgtctcca aggattatcc gcgagtttga
    20761 tcgcgagctg aaaagcgctg cggatgtggt cttgtattcc gggcgcaaac tatttcagga
    20821 agccactgag cctaaccggt actttttaga acaggcagtg gattttgagc acttctcttc
    20881 cctgcctgac gccattgtcg aagagcttga aggtgtgccg cacccgatcc ttggctattt
    20941 tggcgcgatg gattacgtta tggacactga gctgatagaa gaagttgcaa ggttgcgtcc
    21001 tgagtggcag tgggtattta tcgggcttaa atcgaacttg atacagatta cggcccccaa
    21061 tgtcaggttc ctcggatcaa aaccctacaa agatcttccc aaatacattc gttattttga
    21121 tgtctgtgtt cttccgtggc ggcactcaaa tgcttttaca agttacggaa gcgcaatcaa
    21181 ggtccgggag tatctggcga cgggtaaacc agttgtaatt tctccccttt atgaatatct
    21241 ggaaattcct ggtttgcgta tctatcgcgg caaagacgag tttattcgta atgtagagga
    21301 tgcgcttgca gaatgcggcc ccaaacaatc tttggcccgc cagagtgtgg taaagaattg
    21361 cacgtgggac gtaagggcac gcgagttagc tgaattagtc cagatggtgc tggacggcaa
    21421 acaatatgta ttccagaatt atgctccatg gataacaaac agagcagaga ggattaagcg
    21481 atctccagta atggaaggcc cggaggcgta atgaaagcat tgcttgaaga catgaaatcc
    21541 ggtgaagtag caatgcacga agtgcctgct cccgaaccgg cgcacggttg cgttcttgtg
    21601 cgcaccgcgt tttctgcaat cagcgcaggc acggagaggg taaagatcga atccggacaa
    21661 aagtcattgt taggaaaagc aatggcccgt cctgatcttg taaaacaagt aatagacagc
    21721 gcgcgtgcaa acggcataaa aaccgcatgg cagaaagtcc aggcgcgttt agatacactt
    21781 tctactctag ggtattcatg ctcgggctat gtaatcgaag tcggcgaagg cgtcactgaa
    21841 tttcatcctg gagaccgcgt cgcctgcgca ggcgctggat atgcgaacca ctgcgagatc
    21901 aatgtcgtcc cgaaaaacct ggtagcgcgt gttccagaca atgtggacct ggatgccgcc
    21961 gcattgacta caattggcgc cattgccatg cagggtgttc gccaggcaaa tgtcaccttt
    22021 ggagaaacta tagctgtgat tggcgctgga ctggttggca ttctcgcaat ccagattttg
    22081 cgcgctgccg gttgcagagt cattgctatt gatctttcac cagaccgggc gaaaaaggct
    22141 gccgctctcg gtgcgcatct cgggttaagc agtgttgacg ctggcatgga aagcgccatt
    22201 tatgcatttt cccgttatgg agtggatgca gccatcatta cagcagccac aaaatccgca
    22261 gaacccctag aacttgcggc aaggttgctc agagatcgtg gccgcatcac gattgttggt
    22321 gacgtgggaa tgggcgtctc gagggggaat atgtacatga aagagctttc cctgttgatg
    22381 tcacgctcgt atggtcctgg acgttatgac cctgaatatg aagaagaggg gcacgattat
    22441 ccgattggtt atgtgcgctg gacagaaaag cgcaacatgg aagcatttct ggacctgctc
    22501 tcctgcggta cgctgaatgt aaaacctttg ctcgaacacc gttatccggt agagaaagga
    22561 ggaaaggctt attccgacct tacaaccgga atatatacag gaatcattga ctatcatgct
    22621 ccatctggtc ctacatcgtt tgcgcggcct gccgatgcaa tacagtcccc tcgacctcgc
    22681 gcatctggca tggtcagaat aggatgccta ggagccggat cgttcgcccg ggggatcatc
    22741 tttccccatc tgcgcgcttt aaagcaggtc ctatttgaat ccgtagcaac caactcgggt
    22801 gttgctgctg aatctgcacg caaagggttt ggatttcacg ttgcagaaac accatctgcg
    22861 ttattagcta accctaatgt agatatggcc tttgttctta cccgccatga tagtcatgct
    22921 ttttacgtat gctccgcact tgagcaagga aaagcagtat ttgtagaaaa gccattggca
    22981 acggacctgg agcaattgga tgcggtaaaa gctacctacg agagaatgat cgcacaagga
    23041 aaacatcctt ttcttatggt tggattcaat cgcaggtttg ctccgtttac cgaaaaggta
    23101 tgtaactttt ttgcgggccg
//
LOCUS       DASRBV010000107         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_211878, whole genome shotgun sequence.
ACCESSION   DASRBV010000107 DASRBV010000000
VERSION     DASRBV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211878"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFA02_04230"
     CDS             complement(<1..1505)
                     /locus_tag="VFA02_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZU09086.1"
                     /translation="MSQHRRPALEELGDFTTTISVVPMSLLAILIGVLGACIAWFLIR
                     LIGLFTNLFYYHRVSVSFVSPAANQLGAYAVLVPVIGSIVVGLMARYGAERIRGHGIP
                     EAIESILINGSKVRPRLAVLKPLSAAISIGSGGPFGAEGPIIMTGGAFGSLISQFFHL
                     TSMERRTLLVAGASAGMSATFAAPLSSVLIGVELLLFEWKPRSAIPVALSSATACIVR
                     RYLLGLGPLFPVSAHPVFIGPAGIGGCALAGLAAGILSALLTLGVYACEDLFRKLPIH
                     WMWWPAIGGVFVGLGGLIFPQALGVGYDTIAVLLKGKVPLAIFAGVLLIKSAIWMISL
                     GSGTSGGVLAPLLMMGAALGGIESNFLPDFGPGFWPLVSMGAILGGTMRSPFTGIVFA
                     IELTHDLNMLTPLLVACFLAHAFTVLTLRRSILTEKISRRGYHLSREYSLDPLEILFA
                     REVMRTNIVALPADGTLEEARELVRHHGPGPQGQHLFPVVDANKVLIGVVTR"
     gene            complement(1502..1969)
                     /locus_tag="VFA02_04235"
     CDS             complement(1502..1969)
                     /locus_tag="VFA02_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU09087.1"
                     /translation="MVSPNHSVCPEETPHKDVSIAGYRQLAEIRYRIRQFLRFSEEAA
                     RSHGIEPQQHQLLLAIKGLPEDLRPTVTVLSQRLCLRHHSTVELVDRLVEHGAVVRRP
                     GKQDRREVLIELTPHGEALLHRLSVLHQQELRVSGPALSDALLAFARQWRKSA"
     gene            2136..4025
                     /locus_tag="VFA02_04240"
     CDS             2136..4025
                     /locus_tag="VFA02_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZU09088.1"
                     /translation="MNGHDRVSGVLHRNKRGWLNNEPLLVLGFALLTLYFARDLLIPF
                     AMALTLNFLLAPAVIQLEKLRIRRIPAVILVVITASAILGGVGWVVARQVLGVASDLP
                     NYRANIHDKLTSIHAPARGPIANAMSGLKALRQEISGTPAPQPLPSPSEVEKTRRNRR
                     GREAELQRQETNPNPQPVVVVPPKISDWAYAQQILKPVIKPLGMMGMVFVFTIYMLLK
                     REDLRNRVLLLAGMGRLNVMTQALNDAATRISSYLLLNVLVNASYGLVFGVGLFLLHV
                     PNATLWGVLLAILRMVPYVGMILGGGLPIAFAFAVFPGWWTPLVVLAFFIVLEVAVSN
                     FIEPWLYGSHTGISPLALVTTAMVWTLLWGVPGLILSTPLTVCLIVMGRYVPQMSFLH
                     ILLGDEAQLAPEAHFYERLLAMDQTEAHHIADKFLEGHDLVHLYDEVILPALSLAEQD
                     RHKGFLDDARSAFLFQSATELVTELTDYHPAEQENENATAQARACPVVCVPANDQADE
                     LAAVMLAQLLERKGHKTILLHANTLSPEILGRLAEEPGTTVCISALPPFAFVHARSLC
                     QRIRQFLPDNRIIIGLWGATGNAVALRERFGAVRPDGVATTLSEAIRLAPQCEGVSSA
                     IPQQV"
     gene            complement(4048..>4876)
                     /locus_tag="VFA02_04245"
     CDS             complement(4048..>4876)
                     /locus_tag="VFA02_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU09089.1"
                     /translation="EELERARRLVLKRHRNLHPDDVFVVAADLRNSPEAESLVRQVTE
                     RFGRVDILVNNAGIITAGPVENQAVEEFHNAMDANFFSALHCTLAVLPQMLARKSGAI
                     VNVTSIGGKIAVPHLLPYTASKFAAVGFSEGLYAELRSKGVRVTTVCPGLMRTGSHLN
                     ALFTGDAEREYRWFSLSAGLPGVSTSAAKAAAKIVRAVIAGRSEIAITPQAILAARLS
                     NLCPEATLRVMSAMNTALPKSVPGQSRHYKGTEVRTKEIRPALTLASAAAKRYNQVP"
BASE COUNT         1032 a         1309 c         1573 g          962 t
ORIGIN      
        1 cgtgtgacta cgccgatgag gactttgttt gcgtccacaa cgggaaagag atgctgaccc
       61 tgcggacctg gcccatgatg gcggaccagt tcgcgtgctt cttcaagggt gccgtcggcg
      121 ggcagggcga cgatgttggt acgcatgacc tccctggcaa agagaatctc gagtggatca
      181 agactatatt cgcggctgag gtggtatccg cggcgggaga ttttctcagt caggatggag
      241 cgccgcagtg tgagcacagt aaaggcatgt gccaggaaac aggcgaccag cagcggcgtg
      301 agcatgttca ggtcatgggt cagctcaatg gcaaagacga ttccggtaaa aggcgagcgc
      361 atggtgccgc caagaatcgc gcccatgctt accagcggcc agaagccggg gccgaagtca
      421 ggcaggaaat tgctttcgat accgccgagg gccgcgccca tcatgagcaa cggggcgagc
      481 acgcctcctg atgttccgga accaagggag atcatccaga tggcggactt gatcagtagc
      541 actccggcaa aaatggcgag aggaactttg cctttcagca gcacggcgat tgtgtcataa
      601 ccgacaccta atgcctgggg aaagatcaat ccgccgaggc cgacaaaaac gccgccgatg
      661 gcgggccacc acatccagtg aatgggcagt ttgcgaaaga ggtcttcgca ggcgtagacg
      721 ccgagcgtga gcaacgcaga aagaattccc gcggccagac ctgcaagggc gcagcctccg
      781 atgcctgccg gcccgataaa cacgggatgc gcagagacgg gaaacagcgg gccaagtccc
      841 aataaatagc gccggacgat gcacgcggtt gcgcttgaaa gggcaacggg aatggcgctg
      901 cgcggcttcc actcaaagag cagaagctcg acgccgatga gcacagaaga aagcggagcg
      961 gcaaaggtcg cagacatgcc tgcggatgct ccggcgacga gcagcgtgcg ccgctccatg
     1021 ctggtgaggt ggaaaaactg actgatgaga gagccgaaag ccccgcccgt catgataatg
     1081 gggccttccg cgccaaaggg gccgccggaa ccaatggaga tagcagcgga aagcggctta
     1141 aggacagcga ggcgcggacg caccttgctg ccattgatga ggatggactc aatcgcttca
     1201 ggaatgccgt ggccgcgaat gcgctccgct ccataacgcg ccatgaggcc caccacgatg
     1261 gaccctatga caggaacgag gaccgcatag gccccaagct ggttggcggc aggagagaca
     1321 aaggagacac tgacgcgatg gtaatagaaa agatttgtga acaggccaat gaggcggatg
     1381 agaaaccacg caatgcaagc gccgaggacc ccgatgagaa tggccagaag cgacatgggg
     1441 acgacgctta ttgtcgtggt aaagtcgccc agctcctcaa gggccggcct gcggtgttgg
     1501 ctcatgcgct ctttctccat tggcgcgcaa aggccagcag cgcgtcagac aaagcaggac
     1561 cggagacacg cagttcctgc tgatgcagaa cagaaagccg gtgcaggagt gcttctccgt
     1621 gaggagtgag ttcgatcagc acctcgcgcc ggtcctgttt gccgggccgg cggacgactg
     1681 ctccatgctc gaccagacgg tctaccagct cgactgtgct gtggtgccgc aggcaaaggc
     1741 gttgcgagag cacggtaacg gtcgggcgaa ggtcttcagg aagtccttta atcgccagca
     1801 gaagctggtg ctgttgcggc tcgattccgt gggaacgtgc ggcctcttca ctgaagcgga
     1861 ggaactggcg gatgcggtag cggatttcgg caagctggcg gtatcccgca atggaaacat
     1921 ctttgtgggg cgtttcttcc gggcaaacgg aatggtttgg ggaaaccatc ggggaagccc
     1981 tcgccaggaa atatcgtaac acgatataag ggcgtgcgcg ccagagaaaa aacaccgggt
     2041 tgattgtcat caaaacagag ggattcgcac aaaacgggtg ttgaggaaag agagagagag
     2101 gggcaatcgg cttatcctat agattccgca cctttatgaa tggacacgat cgcgtttccg
     2161 gcgtgctgca cagaaacaag cgtggatggc tgaacaacga gccgctgctg gtgcttggtt
     2221 ttgcgctgct tacgctctat tttgcgcgtg acctgctgat tccgtttgct atggcgctga
     2281 cgctgaactt cctgctggcg ccggcggtca ttcagttgga gaagctgcgg attcgccgga
     2341 ttccggcggt gattctggtg gtgataacgg cgtcggccat cctgggagga gtaggttggg
     2401 tggtggcgcg tcaggtgttg ggtgtggcca gcgacctgcc caactaccgc gccaatattc
     2461 atgacaagct gacgagcatt catgcgcccg caagaggccc gattgcgaac gcgatgagcg
     2521 gcctgaaggc attaaggcag gaaatctccg gtacgcccgc gccgcagccg ttgccttctc
     2581 cttcagaagt ggagaagacg cggcgcaacc gccgcgggcg cgaggccgag ctgcaaaggc
     2641 aggaaacgaa cccaaatccg cagccagtgg tcgttgttcc acccaaaata agtgactggg
     2701 cctatgcgca gcagatactg aagcctgtga tcaagccgtt gggcatgatg ggcatggtct
     2761 tcgtctttac catttacatg ctgttgaagc gcgaagacct gcgcaaccgt gtgctgctgc
     2821 tggccggaat gggccgcctg aatgtgatga cgcaggcgct gaatgatgca gcaacccgca
     2881 tcagcagcta tctgctgttg aatgtgctgg tgaatgccag ttatgggctg gtgttcggcg
     2941 ttgggctgtt tctgctgcac gttcccaacg ccacgctctg gggggtcctg cttgcgattc
     3001 tgcgcatggt gccctatgtg ggcatgattc taggcggggg actgccgatt gcttttgcct
     3061 ttgctgtgtt tcccggatgg tggacgcctc tggtggtgct ggcattcttt atcgtgctgg
     3121 aagttgcggt gagcaacttt attgaaccgt ggctttatgg cagtcatacg gggatttcgc
     3181 cgctagcgct ggtcacaaca gcgatggtgt ggacgctgct ctggggggtc ccggggctga
     3241 ttctttccac tccgctgaca gtgtgcctga ttgtgatggg gcgctatgtt ccgcagatgt
     3301 ccttcctgca cattcttctg ggcgatgaag cgcagcttgc cccagaggca catttctatg
     3361 aacgtcttct ggccatggac cagactgagg cgcaccacat cgcagacaag tttctcgagg
     3421 ggcacgatct tgtgcatctt tacgatgaag tgatactgcc cgcattgagc ctggccgagc
     3481 aggacaggca taaagggttt ctagacgacg cccgcagcgc gtttcttttt caaagcgcga
     3541 cggagctggt gacggagctg accgactatc atccagccga gcaggaaaat gaaaacgcga
     3601 ctgcacaggc gcgggcctgt ccagtggttt gcgttccggc caacgaccag gccgatgagc
     3661 tggctgccgt gatgctggcg cagttgctgg agcgcaaggg acacaagacc atcctgctgc
     3721 acgccaatac actttcgccg gaaattcttg gacgccttgc cgaagagccg gggacgacgg
     3781 tctgtatctc ggcgctgccg ccgtttgcct ttgtccacgc gcgatcactt tgccagcgca
     3841 tccggcaatt tctgccggac aacaggatca tcatcggctt gtggggagcg acaggaaacg
     3901 ccgtagcatt acgggagcgc tttggcgcgg tccgcccaga cggcgtggcc acaacgcttt
     3961 ctgaggccat tcgtctggcg ccgcagtgcg agggtgtttc ctcagctatt ccgcaacaag
     4021 tgtaagagtg gggcccagct tgttccatca ggggacctgg ttatatcttt ttgctgcggc
     4081 ggacgccagc gtgagtgctg gcctgatttc ctttgtgcgg acctcggtgc ccttatagtg
     4141 acgagattgg cccgggaccg atttgggcaa agcagtattc attgcggaca tgacccgcag
     4201 ggtcgcctcg ggacacaggt ttgagagccg ggcagccagg attgcctgcg gagtgatggc
     4261 aatttcgctt ctgcccgcaa tgaccgcacg cacgatcttt gctgctgctt tcgcagcgga
     4321 tgtggagacc ccgggaagcc ctgccgagag gctgaaccag cggtattcgc gctcggcgtc
     4381 tccggtaaac agcgcgttca gatgagagcc ggtgcgcatg aggccggggc agactgtggt
     4441 gacgcggaca cctttcgagc gcagttctgc gtagagtcct tcagagaacc cgacggcggc
     4501 gaatttgctt gcggtatacg gcagaagatg cggcacggca atttttccgc caatggacgt
     4561 gacattgacg atggcgccgc ttttgcgcgc cagcatctgc ggcaggacgg cgagggtgca
     4621 gtgcagggcg gagaagaagt tcgcgtccat tgcgttgtgg aactcttcca cagcctggtt
     4681 ttcaacgggg cccgcggtga tgatgcccgc gttattcacc agaatgtcaa cgcggccgaa
     4741 gcgttcggtt acctgcctta ccagtgattc tgcctctgga gaatttcgca ggtcggcagc
     4801 gacaacaaaa acatcgtccg ggtgaagatt gcgatgtctc ttcagaacaa gtctgcgggc
     4861 gcgctccagt tcttcc
//
LOCUS       DASRBV010000108         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_309406, whole genome shotgun sequence.
ACCESSION   DASRBV010000108 DASRBV010000000
VERSION     DASRBV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309406"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFA02_04250"
     CDS             complement(<1..669)
                     /locus_tag="VFA02_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZU09090.1"
                     /translation="MSTIASPALRKTALNAVHRQMGAKMVDFGGWDMPVEYSGLIAEH
                     MAVRTGVGLFDVSHMGDIQLRGPESFQAVQHICMNDAAKLQVGQAQYSAMLYPQGTFV
                     DDVIVHKFSDNDYLIVINAGTREKDYNWVRTNAQKFHCHVSDYSDYYTQLAIQGPKAA
                     ETLQKLTSVDLSTIKFYWFKWGTVCGLPNTLIARTGYTGEDGFEIYIPSDEATSKRVW
                     EEVLE"
     gene            complement(843..1700)
                     /locus_tag="VFA02_04255"
     CDS             complement(843..1700)
                     /locus_tag="VFA02_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZU09091.1"
                     /translation="MASTVWKGYISFGLISVPIRLFVAAREEHVSFNQIHNVCGTRVK
                     QQLYCPTCERVVERNEIAKGYPIDKDTYVQVTPEELKTLEAESSETMEIVQFVKLEDV
                     DPLYYQTSYYTVPEDPGRRAYTLIMKGMEHLKLGAIAKITLHQREQIVMIRPYHHGLV
                     LHTLYYPAEVREIAEYGVDEDSIKLQKPEIELAEQFMKQLTAKFKPDEFKDEYQDRVL
                     KLVEEKEAGMVPKAEKRQKKKLAPVINLMDALRKSMEEQEEAVGKKAPQRETAPAPAK
                     AAGRRKKVG"
     gene            1929..>5470
                     /locus_tag="VFA02_04260"
     CDS             1929..>5470
                     /locus_tag="VFA02_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09092.1"
                     /translation="MKQCWKFVFLALLFASINAALAQSTSRVAGTVQDSSGAVVPGAT
                     VTLTNEATRVSVQTTTSSAGAYVFDGIIPGTYTVTVEQSGFSTYVSTGNVLTIAQPLA
                     VNVTLQVGQATQKVEVSAAAETVETETSGNLGALIDMKAVETLPVVGSRGRSPLQLVE
                     LVPGVVDGGPYNSAGANIEGGGVIVNGSRDRAWNYTLDGIDINETSAPGSNFSPLRAN
                     PDSISGFRVITTNATAQYGVTSGGQVIMDTKEGTNDFHGNVFWFYQTPGLNANDPGFK
                     ENGQGRPQFIQHIPGFSIGGPIIRNKTFFFVNTQFLHASQDLYVNSTVLTQSARNGIW
                     RYVTQPANCGDNCPHNTPYGQSGASVDANGNPVVPINSYNIAANDPQHIGLDPAIQSF
                     IGLTPLPNNFQIGDGLNEAGYQFLAPEEERQVDFVVRIDHQFNQKNSIYGRWAHGHQN
                     TIGDSGNGGLQPFPNAPNIVNTLRQPRNLAIGWRYTPGPTVTNELIAGMNRFIFNFIN
                     PDPNYLHNPPYLFNNGLALPRQNYVGNLRVLTDIQLLDNFTLLHGAHAWKFGANLRYQ
                     RHIDDRGSIGNYDAQPLWLFDTGVARVDQSTGFTLPSDINGTYDLPTLKGDINNLLGR
                     VGTLEQGFVAANSNAYAPAGTHLRDDFRMPEYDFYVQDTWKLRPNFVLDAGLRWEIKL
                     APRTSANFNLHPNQPVDITAPATTTMAWVPGKIYKDAWHDLAPTIGFAWDVRGNGKTS
                     IRGNYRLAHDRINTFVLSSTIFQNLPGEALSAPYTGDAGMRVSQGGIGVPQPSARPID
                     FRQPPPFSTGGNTVVDPNWRPAQVSEWSMSIQRQITTDTVAEIAYVGHHAVHLFGAYD
                     ANQAEIFRNGFLQAYLTAQSGQDSPLIDQLLQNDPSRPAGEPGSQYLFDPNTSPYIGD
                     VNLGSVAMVAEDIGLALDANGNSINPNNLNFFYRFPQYAGGLIVLDSNDWSNYNALQA
                     SWRGRVFHDFSFQANYTWSKSMDTRSFDPTFATIVGGSSTYGSSSTPFNIYNRKLNYG
                     PSDADRTHVAQGVWTYEIPFGSNGRWAKHWGALADRLIGGWEIAGDAIMESGRPTTIY
                     SPAYTLTNLVRTPANCDHCSRNMLKVHRDPSSGLLTYVTPAQFAKFSSPGPGQFSNIG
                     RNYFRLGPYSVLNLSVGKVTHIAESHSLELRIEM"
BASE COUNT         1201 a         1761 c         1311 g         1197 t
ORIGIN      
        1 ttccagcacc tcttcccata cgcgcttact cgtcgcttca tccgaaggga tgtaaatctc
       61 aaagccatct tcgcccgtgt atcccgtgcg ggcaatcagc gtattgggca ggccgcagac
      121 cgtcccccac ttaaaccagt aaaacttaat tgtggaaagg tccacgctgg tcagcttttg
      181 cagcgtttcc gccgccttcg ggccttgaat cgcaagctgc gtgtagtagt cgctgtagtc
      241 gctgacgtga caatgaaact tctgtgcgtt tgttctgacc cagttgtagt ctttctcgcg
      301 cgtcccggca ttgataacga ttagataatc gttgtctgaa aatttatgga caatcacgtc
      361 atccacaaac gtcccctgcg gataaagcat cgccgaatac tgcgcctgcc ccacctgtag
      421 tttcgcagca tcgttcatgc agatgtgctg gacggcttgg aacgactccg gcccgcgcaa
      481 ctggatgtcg cccatatggc tcacatcgaa cagccccacc ccggtccgga cagccatgtg
      541 ctcggcgatc aggccggaat actccacagg catgtcccac ccgccaaaat ccaccatctt
      601 ggcgcccatc tggcggtgta cggcatttaa agcagttttg cgaagcgcag gagaagcaat
      661 tgtagacaca gcattgtcct tcaggcgtaa attgcagaac tttttacgct aaagcatttc
      721 gcgggtggtg taaaccggag aggcccgccg ctgcatccat cccgtgtcca gagatatgat
      781 ttaccgcaaa gaagatagaa accacggagc catcgcagac ctccgtgctc tctgtggtgt
      841 tcttacccaa ccttctttcg ccgtcccgcc gctttcgcag gtgccggagc agtctcccgc
      901 tgcggagctt tcttccccac cgcttcttcc tgctcctcca tgctcttcct cagcgcgtcc
      961 ataagattga tgaccggggc cagtttcttc ttctgccgct tctccgcttt cgggaccatt
     1021 cccgcttcct tctcttccac cagcttcagc acgcggtcct gatactcatc tttaaattca
     1081 tctggcttga atttcgccgt cagttgtttc ataaactgtt cggccagctc aatctcaggc
     1141 ttctgcagtt tgatggaatc ctcatccacc ccatactcgg caatttcgcg cacctctgcc
     1201 ggatagtaca gcgtatgcag caccagccca tggtggtaag gccggatcat cacaatctgc
     1261 tcacgctggt gcagcgtaat tttggcaatc gcgcccagct tcaggtgctc catgcctttc
     1321 ataatcagcg tataggcgcg gcgccctggg tcttccggaa cagtgtagta agaggtctgg
     1381 tagtaaagag ggtctacatc ctcaagcttc acaaactgca cgatctccat cgtctccgac
     1441 gattcggcct ccagcgtctt cagctcttcc ggtgtgactt ggacataagt gtccttgtca
     1501 atgggataac ccttggcgat ttcattccgt tccacaacac gctcgcaggt cgggcaataa
     1561 agctgctgct tcacccgcgt tccgcaaaca ttgtggatct gattgaaact cacatgctct
     1621 tcgcgcgccg ccacaaacag gcgaatcggg accgagatca gtccaaagga aatgtagcct
     1681 ttccagactg tagaagccat aaaaacgcaa accctttcgg caacaataag ttgggatgaa
     1741 attgtacacc gccgggatgg ccctttgcac cagaaatgat gcgctccgtg ggcctttcgt
     1801 acccgcttct cagttatctc gaaagcaaac tttcttcctg aaaaatagtt cttgttttac
     1861 taacagatgg ttgtatagtt atccatcaaa ccggattttt aaacgtttct atccctgagg
     1921 ccgcccccat gaaacagtgt tggaagttcg tgtttcttgc ccttctcttt gcatccatca
     1981 atgctgcgct ggcgcaatcc acctcgcgag tggccgggac cgtgcaggac tccagcgggg
     2041 ccgtcgtccc cggcgccacc gtgaccctta ccaatgaagc caccagagtt tctgtccaga
     2101 ccaccacctc ctccgctggt gcatacgtct tcgatggcat catccccggc acctacaccg
     2161 tcacggtcga gcagtctggg ttctccacct acgtctccac cggaaatgtg ctgaccattg
     2221 cgcaaccgct ggctgtgaac gtcaccctgc aggttggcca ggccacgcag aaagtagaag
     2281 tcagcgctgc cgccgagacg gtcgagactg agacctccgg caaccttggc gctttgattg
     2341 acatgaaggc cgttgaaacg ctgcccgtcg taggctcgcg cggacgcagc ccgctccagc
     2401 tcgtcgagct ggttcccggc gtcgtggacg gtggccctta caactccgca ggtgcaaaca
     2461 tcgaaggtgg cggcgtcata gtcaacggat cgcgcgaccg cgcctggaat tacaccctcg
     2521 atggtattga catcaacgaa accagcgccc ccggctcgaa tttctcccct ttgcgtgcca
     2581 atcctgactc catctccggc tttcgcgtca tcaccaccaa cgccaccgcg caatacggcg
     2641 tcaccagcgg cggacaggtc atcatggaca ccaaagaagg cacaaatgat tttcacggga
     2701 atgtcttctg gttctatcag actccaggac tcaacgcgaa cgatcccggc tttaaggaaa
     2761 atggtcaggg caggccgcaa ttcatccagc acatccccgg cttcagcatc ggcggcccca
     2821 tcatccggaa caagaccttc ttttttgtga acacccagtt cctgcatgct tcgcaggacc
     2881 tgtacgtaaa cagcaccgtg ctcacccaat ccgcgcgcaa tggaatctgg cggtatgtga
     2941 cccagccggc caattgcggc gacaattgcc cccacaacac tccctatggc cagtccgggg
     3001 cctctgtaga tgccaacgga aaccccgttg ttcccatcaa cagctacaac attgctgcta
     3061 atgacccgca gcacatcgga ctcgatccgg cgattcaaag ctttatcggg ctgactcctc
     3121 tgcccaacaa ctttcaaatt ggggacggcc tgaatgaagc cggctatcag ttcttggcgc
     3181 cggaagaaga gcgtcaggtg gatttcgtcg tgcgcattga ccaccagttc aatcagaaaa
     3241 attccatcta tggacgctgg gcccacggtc atcaaaacac cattggagac agcggcaatg
     3301 gtggccttca gccctttccc aatgctccga atatcgtcaa tacgctgcgc cagccgcgca
     3361 atctcgccat cggatggcgc tatacgccag gccccacggt caccaatgag cttatcgcgg
     3421 gcatgaaccg cttcatcttc aatttcatta atcctgaccc gaattacctg cacaacccgc
     3481 cctacctgtt caacaacggg ctggcgcttc cgcgacaaaa ttacgtcggc aatctgcgcg
     3541 tgctcactga cattcagcta ctcgacaact tcacccttct gcacggcgcg catgcatgga
     3601 agttcggcgc caacctccgc taccagcggc atattgatga ccgtggctcg atcggcaatt
     3661 acgacgcaca acccctctgg ctcttcgata caggagtggc ccgcgtagac cagagcactg
     3721 gttttacgct gccctctgac atcaacggca catacgatct ccccacgctg aaaggcgata
     3781 tcaataacct gcttggaaga gtgggcaccc tcgaacaggg ctttgtcgca gccaattcaa
     3841 atgcctatgc gccggcggga acgcacctgc gcgacgattt ccgtatgccc gaatacgact
     3901 tctatgtgca ggacacctgg aaactgcggc caaactttgt cctcgacgca gggctacgct
     3961 gggaaataaa actcgccccc cgcaccagcg ccaacttcaa cctgcatccc aaccagcccg
     4021 tagatatcac agctccggca acgaccacga tggcctgggt gccgggaaag atttacaaag
     4081 acgcatggca tgatcttgct cccaccattg gctttgcctg ggacgtgcgt ggcaatggca
     4141 agacatccat acgcggcaat taccgcctgg cccacgaccg gataaacacc ttcgtcctct
     4201 cctcaaccat cttccagaac ctgcccggtg aggccctctc cgcaccttat actggcgatg
     4261 caggaatgcg cgtctcccag ggcggcattg gagtacccca accctcggcc cgcccaattg
     4321 acttccgcca gcctcccccc ttttccaccg gtggaaacac ggtcgtggac cccaactggc
     4381 gtccggcaca ggtctctgaa tggagcatga gcatccagcg ccagattaca accgacaccg
     4441 tcgcggagat cgcttatgtg ggacaccacg ccgttcacct cttcggtgcc tatgatgcca
     4501 atcaggccga gattttccgc aatggcttcc tgcaagcgta tttaacagca cagtccggac
     4561 aggacagccc cctgattgat caactgttgc aaaacgatcc cagccgtccc gcgggtgaac
     4621 ccggctcgca gtatcttttt gaccccaaca ccagccctta catcggagac gtgaatttgg
     4681 gctcggttgc catggtggcc gaagacatcg gattggcact ggacgccaat ggcaactcca
     4741 tcaatccgaa caatctgaac ttcttctacc gcttcccgca atatgccggc ggcctcatcg
     4801 tgctggacag caatgactgg tctaactaca acgcattgca ggcgtcctgg cgaggacgtg
     4861 ttttccacga tttcagcttc caggcaaatt acacctggtc caagtccatg gacacgcgct
     4921 cgttcgatcc aaccttcgcc accatcgtgg gcggcagcag cacctatggc tcatccagca
     4981 cgccgttcaa tatttacaac cgtaaactca actacggccc ttctgacgcc gaccgcaccc
     5041 atgtcgccca gggcgtctgg acttatgaaa tccccttcgg cagcaatggg cgatgggcga
     5101 agcactgggg tgctctggcc gaccgcctga ttggcggctg ggaaatcgcc ggggacgcca
     5161 tcatggaatc cggccgccca accaccatct actcgcccgc ttacacactc acaaatctgg
     5221 tgcgcacacc cgcaaactgc gaccattgca gccgcaatat gctgaaagtg catcgcgacc
     5281 ccagttccgg cctgctgacc tacgtcactc cggcccagtt tgcaaaattc tcctctcccg
     5341 gaccgggcca gttcagcaac atcggcagaa actatttccg gcttgggccc tattccgtgc
     5401 tgaacctcag cgtcggcaaa gtcacccaca ttgcggaaag ccattcactg gaactgcgca
     5461 tcgaaatgca
//
LOCUS       DASRBV010000109        10721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_106503, whole genome shotgun sequence.
ACCESSION   DASRBV010000109 DASRBV010000000
VERSION     DASRBV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106503"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VFA02_04265"
     CDS             <1..1681
                     /locus_tag="VFA02_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09093.1"
                     /translation="SYGAIPAQPHGHSYFRRAPDGAPKILADTVAYSYSIVASRQPVK
                     LGYRRVVRWLWKEYGHPSLLDSPDTQQNVIRNELRSFDSWRKDAWLDYANQVYFGFDC
                     GRKRCGTLKSNRNNSGDWEHAEPDAWFNAWFQTLRTAYGWYIYGRETHNADILSKAES
                     VLNLALSAPQKDGAFPTIYMVNSHQWISDDGWAGYKNDYHAFCMSWTAYWMLRWAEDL
                     VPQRKTEILRFLKPYADFLLEHQLSSGVVPSWYDSTLLQPRAEFRDFNAETAVSALFL
                     ASLGTATGEQKYIVSAERAMDFVTNEVIPRQRWFDFETFLSCARKDYGFYDRWTAQFP
                     QNNLAEIQAPEAWLVLYHATHKREYLDRGVKALDYLLLTQQVWSNPLFSPKLLGGFTT
                     QNTDAEWSDARQCYAAMVLFDYYRETGDFEYLERAVAAARSTFAVAPWENWAHTGYKD
                     GPGALTGFHWGTGSAMTSVEMMSPVLGDAYIDLSSGRGVGFDECSIRDVHVANGWIAF
                     TIESPAKLRTFLVRFAGVNAGRQYRISWNGGPEHVVSGRELMARGYVVRIL"
     gene            complement(1825..2682)
                     /locus_tag="VFA02_04270"
     CDS             complement(1825..2682)
                     /locus_tag="VFA02_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09094.1"
                     /translation="MKKMMTVVLALCTLAAPMFAQTASSNHHALSWIVAYSTIEQMQA
                     QPGGPEVVKKLLADPHTIIVAANPVSGVDEAQRAVSVLSSDDCTAALDKSSNAKLTKL
                     FVDLENWPGTPPSDQKTPQEVTRKCHEHAHDKNRDFVVIAVPAMDLMSRLDPDFKGTQ
                     YQAAIHYDLEGKLATVSDGMEVQLENIEDKPDEFVRTLQTMVHQIREARIKAHQNPDI
                     PIYVGLSTAVVNKPIRADVLVNLLKEDVNRTRGMVTGYWMANPSKKLCPGCDSNPEVA
                     VKLLQSVDE"
     gene            complement(2683..3558)
                     /locus_tag="VFA02_04275"
     CDS             complement(2683..3558)
                     /locus_tag="VFA02_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU09095.1"
                     /translation="MDEWLIKTFEKNRPRLRAVAYRMLGSLTEAEDAVQETWLRLHRS
                     EAGAIDNLEGWLTTVIARVCLDVLRLRKARQEEPLDDHVPDLIISREDGMDPEHEALL
                     ADSVGMALLVILETLAPSERLALVLHDMFAVRFEEVASILGCSTVGARQLASRARRKV
                     QGATIFPDVGLKQQRTVVDAFLAAVRSGDLEALVAVLDPEIVIRSDEGPVRELRGART
                     VAEKAMMFSQFAQRAQPVLVNGVAGLLSWRSDGQVFSVMSFIVRGNRIAEMDVVRNPA
                     RLRRMDMASVIMRRI"
     gene            3662..4249
                     /locus_tag="VFA02_04280"
     CDS             3662..4249
                     /locus_tag="VFA02_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU09096.1"
                     /translation="MTAVLIVSLIVLATFAGVLSAKHEGDAPAQGRRAMICSIAYWLF
                     TVIVAFELAAGALWDLLRIEYVRVVLTHLGYPLYLLVILGVWRVPGALTLLVPRFPRL
                     KEWAYAGAFFDYTGAAASHLWAGDGVGQWIGPLIFAGFTLISWGLRPASRRLPEFAPP
                     VPEKRLVIWGVPILTVAAMMVVAIFTLPKGAPPQS"
     gene            complement(4255..5160)
                     /locus_tag="VFA02_04285"
     CDS             complement(4255..5160)
                     /locus_tag="VFA02_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZU09097.1"
                     /translation="MTRHRQSANKESGFILLAVIFMVALILIALAVAAPRIAKSIQRD
                     KEEELIHRGLQYRQAIKLYYRKFGRYPSSIDQLVNSNNIRFLRKRYLDPMTGKDDWKL
                     VRFGQAHVKAMGLFGQPISNSGTAGSSVLGGGIGQTSGFGGLQQSSSSPFSNSAGTSS
                     SSSINSNSSDNGSAGNSDGSSTPGTSGTSGLSSGNSGLGQSSNGFGNTNQTFGGAPIV
                     GVAIPVEKASIKEYKQQKHYNEWEFVYDPLEDMQRANFFGGTTNVNGTNGTGTNTPGT
                     SSPFSSGPGTSTPTTPTGSSPTSPQ"
     gene            complement(5163..5777)
                     /locus_tag="VFA02_04290"
     CDS             complement(5163..5777)
                     /locus_tag="VFA02_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09098.1"
                     /translation="MALKVGTEDKKKVIIAGALGLVVLVLLVRFLVDFFGGSPTPPPG
                     PPQTVVRPAAPTPAQRPAQSEHAAAKVPGAGASLDPTLHPEWMAQAESLEYTGTGRNI
                     FSQFSAPPVIEQVKAPIRPSQSVAQVPQGPPPPPPIDLKFFGYEAHKDGARKAFLLHG
                     DDVFIASEGDIVDHRYRVLKINPFSVEIEDIPYHNAQPLPLTQN"
     gene            complement(5777..6400)
                     /locus_tag="VFA02_04295"
     CDS             complement(5777..6400)
                     /locus_tag="VFA02_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09099.1"
                     /translation="MNLRRLFSLLNLHIAGVAVLLVLNLVLLTRLFFAWHDASSDKSE
                     EYESLHMTYMQLQAQMSHLQGLPQKLDQARTGADKFFDQRFAPNYSTMAGELGLLAAK
                     NNVRLTRAQYTPTPAIHGLTEVRIDASLSGEYAPLMRFINGIERDKNHVFFIINQVAF
                     NGQQGGLVNVRLRMTTYLRSSSDLPPATNEQPSMNTEAAALRFPEVR"
     gene            complement(6397..7053)
                     /locus_tag="VFA02_04300"
     CDS             complement(6397..7053)
                     /locus_tag="VFA02_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HZU09100.1"
                     /translation="MRITINLASHPYVELRPIYTRLRLWMLVLAVLAVPLGWLLHIEK
                     QRAAAATAHVRAMQTRVDQLQRQQQSYQTLMRQPQNAAVLDQSDFLNNLFRQKSFSWT
                     ATMTDLETVLPAGVQVQSIDPQMSPDGHVTIRMRVTGQRERALDLVKNLERSKHFASP
                     RLAGEALATSSQGNQPMPVSASATDVNFDILADYRPFPGDEKAAAKQPVQQAQKGAEQ
                     "
     gene            complement(7054..7932)
                     /locus_tag="VFA02_04305"
     CDS             complement(7054..7932)
                     /locus_tag="VFA02_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09101.1"
                     /translation="MPNTHRRPPLACEFLPDGIVAARMAEGEQPVTAFASLPEGALVP
                     SLRAANIADRAAVVAALKRAVEAVESRERHITLVVPDTAARVLLLDFDTLPAKPAEAL
                     PVVRFRLRKLVPFEIDDAAVSYQIMRQSAEEMRVLVTAMPHAVLTEYEEVARLAGLEP
                     GAVLPSTLASLAALSQQGAALVVNRNGRSVTTAITRGEELLLHRSLDLPADEAQCESD
                     VRQAVAVSLAYFEDTLSSTPQEVFYAGPGGAAELARVLEQEQFRIRDLVSPANVGETT
                     NVPRGLMAGVRGALAS"
     gene            complement(7950..9575)
                     /locus_tag="VFA02_04310"
     CDS             complement(7950..9575)
                     /locus_tag="VFA02_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZU09102.1"
                     /translation="MAEAPALIPIEQDEAEHAKRLAARYRCEFVDLKHFRIQHDLFRT
                     VPVDMMFRYNFVPLEQLEDRLVIAVSDPSRLMVLDEISSLLGQRIVTRVATLSQITDL
                     LKKTEQSQRVLEEASEGLTFDVLSSEDNADENLSIEKLTAEEDISPIIRLVDTTIFTA
                     LERRASDIHIETYDDGVIVKYRIDGVLQQAMAPIAREHHSTILSRIKVMSELDIAERR
                     VPQDGRFRVRYKGRLIDFRVSIMPTVHGENAVLRVLDKESMSEKFRRLTLDVVGFAED
                     DLRRFRRYIREPYGMVLVTGPTGSGKTTTLYAALNEIKSDEDKIITIEDPVEYQIRGI
                     TQIPVNEKKGLTFARGLRSILRHDPDKILVGEIRDQETAQIAINSALTGHLVFTTVHA
                     NNVFDVLGRFLNMGVEPYNFISALNCILAQRLVRTICEYCGRSVRYDEETLVNSGLDP
                     AEWREFDFREGAGCIECGGTGYRGRTAIHELLDLTDPIREMILDKKPGSEVRKLAAKE
                     GMRFLRESALDRVRRGITTLKEINKVTFIEATR"
     gene            complement(9585..>10721)
                     /locus_tag="VFA02_04315"
     CDS             complement(9585..>10721)
                     /locus_tag="VFA02_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZU09103.1"
                     /translation="SAASAEELRQRFSQAGYYVYSVKPRGLTAVRKKVKLETFLIFNQ
                     QFLTLIRAGLPILSSLEMLAKNQKNTNFAAQLQNVAARVRTGESISSAFEAQGGFPLI
                     YTTTLLAGERSGNLEEVLGRYLAFQRVSLAFRKKLQASLIYPALLITLVFGLFIFLIS
                     FVVPRFAQLYDQIGTKLPSITMYLLALGNAAQRYIFYVLPALVLAVYLLFRWSRTDSG
                     AERIDSVRIRLPIFGKIWLKYQVALFSRTLSTLLSGGLPLVPSLETAARSIASKRIAK
                     AVFASVASVREGKSLARSLEGTKVFPELSTEMIEVGESTGALPQMLNSVAEFFEEDVQ
                     TALTASLSLIEPAILIIMGVVVVIILIALYLPIISLSQASGFQT"
BASE COUNT         2252 a         2968 c         3096 g         2405 t
ORIGIN      
        1 ttcttatggg gccattccgg cgcagccgca tggacacagt tatttccgtc gcgctccgga
       61 tggtgctcca aagatcctgg ccgatacggt tgcgtactcc tattcgattg tggcttcgcg
      121 gcagccggta aagcttgggt accgccgtgt tgtgcgctgg ttgtggaagg agtatgggca
      181 tccttccctg ctggattcac cagacacgca gcagaacgtt attcgcaatg agcttcgctc
      241 tttcgattca tggcgaaaag atgcgtggct ggactatgca aaccaggtct acttcggatt
      301 tgactgcggc agaaagcgct gcggaacgct gaaaagcaac cggaacaact ccggcgactg
      361 ggaacatgcc gagccagacg catggttcaa tgcctggttc cagacactgc gcactgccta
      421 tggctggtac atctacggcc gagagacgca caatgcggac atcctgtcga aagccgagag
      481 cgtgctgaac ctggcacttt ctgctccgca gaaagatggg gcctttccca ccatctatat
      541 ggtgaacagc catcagtgga tctctgatga tggatgggcc ggttacaaga atgactatca
      601 cgctttctgc atgtcctgga cagcgtactg gatgctgcga tgggcggaag acctggttcc
      661 gcaacgcaag acggaaatac ttcgcttcct caaaccttat gctgattttc tgctcgaaca
      721 ccagttgtcc agcggtgtag tcccttcatg gtatgactcg actttgcttc agccgcgcgc
      781 ggagttccgc gactttaatg cagagactgc cgtctcggcg ctgtttcttg cttcgcttgg
      841 aacagctact ggtgaacaga aatatattgt ctctgccgaa cgggccatgg actttgttac
      901 gaatgaagtc attcccaggc agaggtggtt tgactttgag accttccttt cctgcgcgag
      961 gaaagattat ggcttctatg accgctggac ggcgcagttt ccgcagaaca atcttgcgga
     1021 gatacaggcg ccggaggcat ggcttgttct ctatcacgcc acccacaaac gcgagtatct
     1081 tgaccgcgga gtgaaggcgc tggactatct actgttgacg cagcaggtgt ggagcaatcc
     1141 gctgttctcg cccaaactgc tggggggctt caccacgcaa aatacagatg ctgagtggag
     1201 cgacgcccgg cagtgctacg ccgcgatggt cttgtttgac tactaccgcg agaccggcga
     1261 ctttgagtat ctggagcgcg ccgttgccgc ggcccgctcc acgtttgctg ttgctccgtg
     1321 ggaaaactgg gcgcataccg gatacaaaga tggaccgggg gcgctgacgg ggttccactg
     1381 gggcacaggc agcgccatga cttcagtgga gatgatgtct ccagtgctgg gcgatgcata
     1441 tattgatctc agtagtggca gaggtgttgg ttttgacgag tgctcgattc gcgatgttca
     1501 cgttgcaaat ggatggatag cattcacgat cgaatcacct gcgaagctga ggacatttct
     1561 tgtgcgcttt gcgggtgtaa atgcgggccg gcaataccgt atctcatgga atggagggcc
     1621 ggagcatgtt gtctccggac gtgagttgat ggcgcgtggg tatgtggtgc gtattttgta
     1681 attccgtgga acgggtggtt atagttccct actcaagcca acatcgggct tccactcctg
     1741 gaaccgcttt tttcaatgct gctggcgggc gctgagcgag ggctgggaac accgcaggac
     1801 aggcaccgat gtcctgcgaa gcccctattc atccacactc tgcaacagct tgacggcaac
     1861 ttccggattt gaatcgcatc cggggcaaag ctttttggaa gggtttgcca tccagtagcc
     1921 ggttaccata cctcttgttc tgttcacatc ttccttaagc aggtttacaa gaacatccgc
     1981 gcgaatcggc ttgttcacaa ctgctgtgct tagtcctaca tagatcggaa tgtcaggatt
     2041 ctggtgggcc ttgattcgtg cctctctaat ctgatgcacc atagtctgaa gcgtccgtac
     2101 aaactcgtcg ggcttgtctt cgatattttc aagctgcact tccattccat cagaaacggt
     2161 ggccagttta ccttccagat catagtgaat ggccgcctgg tactgtgtgc ccttaaagtc
     2221 cgggtcgagc ctggacatca gatccatcgc gggcaccgca atgacgacga aatcgcggtt
     2281 cttatcatgt gcatgttcat gacattttct tgtgacctct tgaggggtct tctggtcaga
     2341 tggaggcgtt cccggccaat tctccagatc cacgaacagt tttgtaagct ttgcattact
     2401 gctcttatcg agagcagcag tgcaatcgtc ggagctcaac accgataccg cccgttgcgc
     2461 ctcgtccacg ccggatacag gattcgcagc cactatgatc gtatgcggat cggccaacag
     2521 cttttttacg acttcaggcc cacccggctg ggcctgcatc tgctcgatcg tggaataggc
     2581 cacgatccac gacaaagcgt gatgattgct cgaagctgtt tgcgcaaaca taggtgctgc
     2641 aagagtacat agtgccagga caaccgtcat catcttcttc attcatatcc tcctcattat
     2701 tactgatgcc atatccatgc gccgcaaccg ggccggatta cggaccacat ccatctcggc
     2761 aattctgttg cctctcacga taaatgacat gactgaaaac acctgtccat ccgaacgcca
     2821 tgacagcagg ccagcaacgc cattcactaa taccggctgg gcccgctggg caaattgtga
     2881 aaacatcatt gccttttccg ccacggtacg cgctccgcga agctctcgca caggcccctc
     2941 gtcggaccgg attacgattt ccggatcgag cactgcgacg agcgcctcta aatcgccgct
     3001 tcggacggcg gcgaggaaag catccaccac tgtcctttgt tgcttcagac caacgtccgg
     3061 gaaaattgtt gcgccttgca ccttccggcg cgcgcgactg gcaagctgcc gtgcgccaac
     3121 ggtagagcag ccgagaatgg acgcaacttc ttcaaagcgc acggcgaaca tatcgtgcag
     3181 caccaaagcg agccgttccg agggcgccag tgtttccaga attacgagca gcgccatacc
     3241 cactgagtcg gcgagcaatg cttcatgctc gggatccatt ccatcttcac ggctgatgat
     3301 caggtcaggt acatggtcat caagcggttc ctcctgccgt gctttgcgta aacgcaagac
     3361 atcaaggcag acccgcgcga tgacggtagt cagccagcct tcgagattat caatggcccc
     3421 ggcttcagag cggtgcagcc gcagccaggt ttcctgtacc gcatcctccg cctcggtcaa
     3481 ggaaccaagc atccggtagg ccacagcacg cagacgaggc ctgttcttct caaatgtctt
     3541 tatgagccac tcatccatct attccaaacc ctgggccatc tgtcacactt atccaatttg
     3601 acccgtcatc tttatgacgg acaagcgcca gtttgtgtga cacgcagagg tgtactcatc
     3661 tatgaccgct gtactgatag tctcgctgat cgtacttgcc acgtttgccg gagtcctctc
     3721 ggccaaacac gagggggacg ctccggcaca agggcggcgg gcaatgattt gctccattgc
     3781 ctattggctg ttcactgtca ttgttgcgtt tgagcttgca gccggggcct tatgggacct
     3841 gttgcgtatt gagtatgttc gtgtggtgct gacgcattta ggctatccac tctatctgct
     3901 ggtcatcctg ggagtctgga gggttcccgg cgcgcttacg cttctggtcc ctcgttttcc
     3961 ccggctgaaa gagtgggcct acgcaggcgc gtttttcgat tacaccgggg ccgcagcctc
     4021 tcatctatgg gcgggcgacg gcgtgggtca atggattggg ccgttaattt tcgctggatt
     4081 tacgctgatc tcatggggat tacgtccggc atctcggcgt ttgcccgaat ttgctccacc
     4141 agtccctgaa aagagactcg taatctgggg agtccccatt cttactgtcg ctgcaatgat
     4201 ggtagtagca atcttcacgc tgcccaaagg cgccccaccg caatcatgac cacgttattg
     4261 cggtgaagtt ggactggaac cggtgggtgt agttggagta gatgtacccg gacctgagct
     4321 aaatggactg gatgttcccg gagtgttggt tccagtgcca tttgtgccat tcacgtttgt
     4381 tgttccaccg aagaagttgg ccctctgcat gtcttcaagc gggtcgtaaa caaactccca
     4441 ttcgttgtag tgcttctgtt gcttgtattc cttaatggaa gccttttcta ctggaatggc
     4501 gacgccaaca atcggcgccc ccccaaaggt ctggtttgta ttgccgaatc cgttgctgga
     4561 ttggcccagg ccagaattgc cggacgacaa gccggatgtt ccactcgttc ccggagtaga
     4621 cgaaccatca gaattgccgg cgctgccatt gtccgatgag ttgctattga tagaagaaga
     4681 cgatgacgtg ccagcactat tgctgaaagg gctggatgaa ctttgctgaa ggccgccaaa
     4741 tccactggtt tgccctatgc ctccgcccag cacgctgctg cctgctgtgc cgctgttgga
     4801 aatcggctgg ccgaagaggc ccatcgcttt tacgtgggcc tgcccaaagc ggacgagctt
     4861 ccagtcgtcc ttgccggtca tggggtcaag ataccgcttg cgcagaaaac ggatgttatt
     4921 gctatttaca agctggtcta tagatgaagg atagcggccg aactttctgt aatagagctt
     4981 gatggcctgg cggtattgga gtccgcgatg gataagctct tcttccttgt cccgctggat
     5041 ggatttggca atacgcgggg ccgcaacggc aagcgcaatc agaataagcg ctaccatgaa
     5101 tatgacggcg agcaggatga atcctgattc tttgttggcg gattggcggt gacgggtcat
     5161 tgtcaattct gcgtcagcgg caagggctgc gcgttgtgat acgggatgtc ttcaatttcc
     5221 actgaaaaag gattgatctt cagcacgcgg tagcggtggt ctacgatgtc cccttctgaa
     5281 gcgataaata cgtcatcgcc gtgcagcagg aatgctttgc gcgcgccgtc tttgtgcgct
     5341 tcgtagccga aaaacttcag gtcaatcggc ggtggaggcg gcggtccttg cggaacctgc
     5401 gctacgctct ggctgggacg aatcggcgcc ttgacctgct cgatcacagg gggcgcagaa
     5461 aattgcgaaa aaatgttgcg cccagtgccc gtgtattcga gcgactcggc ctgcgccatc
     5521 cattccggat gcagcgttgg atccaggctt gcgcctgctc cgggaacttt tgccgcagcg
     5581 tgttcacttt gcgcaggacg ctgcgcagga gtgggcgcgg caggccgaac cactgtctgc
     5641 gggggcccag gcggaggagt ggggcttcca ccgaagaaat ctaccaggaa gcgcaccagc
     5701 agcacgagga cgacaagccc caacgctcct gcaatgatga ccttcttctt gtcttcagtg
     5761 ccgaccttca gggccatcag cgcacctccg ggaagcgcag cgcggcagct tcagtattca
     5821 ttgacggctg ttcattcgtg gccgggggca ggtcgctgct ggagcgcaga tacgttgtca
     5881 tacgcagacg cacgttgacc agtccgccct gctgtccgtt gaaggcgacc tgattgatga
     5941 tgaagaagac atggtttttg tcgcgctcga tgccgttgat gaagcgcatg agcggcgcgt
     6001 attccccgct caggctggcg tcaatgcgga cctcagttag cccatggatg gccggcgtag
     6061 gcgtgtattg cgcacgggta agacggacat tgttctttgc cgccagcaag cctagctcgc
     6121 cggccatggt ggagtagttg ggcgcaaacc gctgatcgaa gaatttgtct gcgccggtcc
     6181 gcgcctgatc tagcttttgc ggaagcccct gcaggtgcga catctgcgcc tgcaattgca
     6241 tataagtcat gtgcagcgat tcatattctt ctgacttatc agaactggcg tcgtgccatg
     6301 caaagaagag cctcgtcaac aaaaccagat tcaataccag caggacagcc acaccggcaa
     6361 tatgcaagtt gagcagagaa aaaagcctgc gcagattcat tgttcggccc ctttctgcgc
     6421 ctgttgcacg ggttgctttg cggcggcttt ttcatctcct ggaaacggtc tgtaatcggc
     6481 cagaatgtcg aagttcacgt ccgttgcaga tgcgctgaca ggcataggct gattgccctg
     6541 gctggatgtg gccagagctt ctcctgcgag ccggggcgaa gcgaagtgct ttgagcgttc
     6601 caggttcttt accagatcaa gggcgcgctc gcgctgtccg gtgacgcgca ttcggatggt
     6661 gacgtgcccg tcgggtgaca tctgcggatc aatactctgt acctgcactc cggcggggag
     6721 gacggtttca aggtcggtca tggtggccgt ccaggagaaa gacttctgcc ggaagagatt
     6781 gttcaggaaa tcagactggt ccagcacagc agcattttgc ggctggcgca tgagcgtctg
     6841 atagctttgc tgctggcgct gcaactggtc cacgcgggtc tgcatggcgc gcacgtgcgc
     6901 cgtggccgca gcagcacgct gtttttcaat gtgcagaagc catccaagcg ggacagcgag
     6961 gacagcaagg acgagcatcc atagccggag ccgtgtataa atgggccgca gctctacata
     7021 aggatgcgaa gcgagattga tggtgatgcg catttagctt gccagcgctc ctcggacccc
     7081 ggccatcagg ccacggggga catttgttgt ttcgcccaca ttggcagggg aaaccaggtc
     7141 acgtatacga aactgctcct gctccaggac gcgcgccagt tcggcagccc cgccagggcc
     7201 ggcataaaac acttcctgtg gcgtagaaga aagcgtgtct tcaaaatagg cgagcgatac
     7261 ggccacagcc tgccgcacgt cactttcaca ctgggcttcg tcagcgggca gatccaggct
     7321 gcgatgcagc agcaactctt cgccgcgcgt gatggccgtg gtaacagaac ggccattgcg
     7381 attgacgacc agcgctgctc cctgctgcga gagagcagcc aacgatgcaa gagtgctggg
     7441 cagaacggcc cccggctcca atccggcaag gcgcgccact tcttcatatt cggtcaggac
     7501 agcgtgcggc atagccgtaa ccagcacgcg catctcctcg gcactctgcc gcatgatctg
     7561 gtagcttaca gcagcgtcgt caatctcgaa cggaacgagt ttgcgcaggc ggaagcgcac
     7621 aaccggaagc gcctctgcgg gctttgccgg aagcgtatca aaatcgagta gcaggacacg
     7681 ggcggcagta tcgggcacaa ccagagtaat gtgtctttcg cgcgattcca cggcctctac
     7741 agcgcgcttc aatgcggcaa ccacagcggc cctgtctgca atatttgctg ctcgcagtga
     7801 aggcacgagg gcgccttccg gcaacgaggc aaatgcagtc acgggttgtt cgccttctgc
     7861 catacgcgca gccacaatgc cgtcaggcag aaactcgcac gccagtggcg gacggcgatg
     7921 tgtattcggc atgaggaatg aaaaagccat cagcgggtcg cctcgatgaa tgtgacttta
     7981 ttgatttcct tgagtgtagt gatgccgcgc cgcacgcgat ccagggccga ttcgcgcagg
     8041 aagcgcattc cttcttttgc tgccagcttg cgtacttctg agcccggctt tttgtccagg
     8101 atcatctcgc ggatgggatc ggtcaggtcg agcagttcgt gaatggccgt tctgccacgg
     8161 tatcctgtgc cgccgcactc aatgcatccc gcgccttcgc ggaaatcaaa ctcgcgccac
     8221 tcggcgggat ccagtccgct gttgacgagg gtttcttcgt cgtaacgcac ggagcggccg
     8281 cagtattcgc aaatggtacg gacaaggcgc tgtgccagaa tacagttcag cgcggagata
     8341 aagttatacg gttcaacacc catgttgaga aatcggccca gtacgtcaaa gacgttgttc
     8401 gcatggaccg tcgtaaagac cagatgcccg gtcagcgccg aattgatggc aatctgcgcc
     8461 gtctcctggt cgcggatttc accgaccagg atcttgtccg ggtcgtggcg cagaatggag
     8521 cgcagtccac gcgcgaaagt caggcccttt ttttcattta cgggaatctg tgtgatgccg
     8581 cggatctggt actctaccgg gtcttcaatg gtgatgattt tgtcttcgtc gctcttgatt
     8641 tcattcagcg cagcgtagag cgtcgtggtt ttaccggagc cggttggtcc ggtgacgagc
     8701 accatgccgt aaggttcgcg aatatagcgg cggaagcggc gcaggtcgtc ctcggcaaag
     8761 ccgacgacat cgagcgtgag cctgcggaac ttctcgctca tggactcctt gtcgagcacg
     8821 cggagcaccg cattctcacc atgcacggtc ggcatgatgg aaacgcgaaa gtcaatgagg
     8881 cggcctttgt agcgtacgcg gaaacgcccg tcctgcggca cgcgacgctc ggcaatgtcc
     8941 agctcgctca tgaccttgat gcgggaaaga atggtcgagt ggtgctcgcg cgcgatggga
     9001 gccatggcct gctgcaacac gccgtcaatg cggtatttca cgatcacgcc gtcatcgtaa
     9061 gtctcgatgt ggatgtctga cgcgcggcgt tccagcgcag tgaagatggt cgtgtctacc
     9121 agacgaatga tggggctgat gtcttcttcc gcggtcagct tctcaatgga gagattctcg
     9181 tccgcgttgt cttcgctcga cagaacatca aatgtcaacc cttcggaggc ctcttcgagc
     9241 acgcgctgcg attgctcggt ctttttcagc aggtcggtaa tctgcgaaag ggtggccacg
     9301 cgggtcacaa tacgctggcc cagcaggctc gaaatttcat cgagcaccat caggcggctc
     9361 ggatcactga cggcaatcac gaggcggtct tccagttgct ccagcggcac gaagttgtag
     9421 cggaacatca tgtccaccgg cacggtgcgg aagaggtcgt gctgaatgcg aaagtgtttc
     9481 aggtccacga actcgcagcg gtagcgcgcc gccagacgct tcgcgtgctc ggcctcgtcc
     9541 tgctcgatgg gaatcaatgc tggtgcttct gccatggtac gtccttatgt ctgaaaaccg
     9601 cttgcctgac tgagcgagat aatgggcagg taaagcgcga tgaggatgat aacaacaacc
     9661 acgcccatga tgattaaaat tgccggctca atcagcgaga gcgatgccgt cagcgcggtc
     9721 tgcacatctt cctcaaaaaa ttcagcgacc gagttgagca tctgcggcag cgcgccggtg
     9781 gattcgccga cttcgatcat ctccgtcgaa agctcgggga aaacttttgt tccttccagg
     9841 ctgcgcgcca ggcttttgcc ttcgcgcaca ctggcaacag aggcgaatac cgctttcgca
     9901 atgcgcttgc tggcaatgga acgggcggcc gtctccagcg aaggaacgag cggcagaccg
     9961 ccggaaagca gggtcgagag agtgcgcgaa aagagcgcca cctgatactt gagccagatc
    10021 tttccaaaga tcggcagccg gatgcgcacg ctgtctatgc gttccgcgcc ggaatcggtg
    10081 cggctccagc gaaacagcag ataaaccgca agcacaagag cgggcagtac atagaagata
    10141 tatctctggg cagcattgcc aagcgccagc aaatacatcg taatcgaagg cagtttggtg
    10201 ccaatttggt cataaagctg ggcaaagcgt ggaaccacga agctgataag gaagatgaac
    10261 agcccgaaga ccagcgtaat cagcagcgcc ggataaatca gcgacgcctg caacttcttc
    10321 cggaatgcca gcgacacgcg ctgaaaggca agataccgcc ccagtacctc ttccagattg
    10381 ccgctgcgct cgccggcgag cagtgtcgtt gtataaatca gcggaaaccc tccctgggcc
    10441 tcaaaggccg aagagatgga ttcgccagtg cgcacacgcg cggccacatt ctgcaactgc
    10501 gcggcaaagt tggtgttctt ctggttcttg gccagcattt cgagcgagct gagaatcggc
    10561 agcccggcgc gaatcagcgt aagaaactgc tgattgaaaa tcagaaaggt ttcaagcttg
    10621 acctttttgc ggacggccgt cagtccacgt ggcttgaccg agtagacgta gtatccggcc
    10681 tgggaaaagc gctggcgcag ctcttcggca gaggctgcgg a
//
LOCUS       DASRBV010000110         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_212212, whole genome shotgun sequence.
ACCESSION   DASRBV010000110 DASRBV010000000
VERSION     DASRBV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212212"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFA02_04320"
     CDS             complement(<1..338)
                     /locus_tag="VFA02_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09104.1"
                     /translation="MPKKTKQPSFDEILANLRGFQFDVREAQGVAGQYRVEKNGCAAV
                     LARSDKGASLVDGPGVVLGGEISHLLDRGFQKFLKTSRLEITATADHLKALHRFSEEL
                     KEAIGSPSFY"
     gene            443..1378
                     /locus_tag="VFA02_04325"
     CDS             443..1378
                     /locus_tag="VFA02_04325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZU09105.1"
                     /translation="MATMTQSLGTLLLDRIGNTPLVRLERIAPHLPGIQLLGKAEWAN
                     PGGSVKDRAASSIVMEAIRKGRLGKGQHLLDATSGNTGIAYAMLGAALGFPVTLCVPA
                     NVSPERKRILNAYGAHVVWTDPNDGSDGAIRKARELAAAGPEKYYYADQYGNDANWQA
                     HYRTTANEIWEQTAGQITHFVAGLGTSGTFVGTTRRLKELNPKIQCISMQPDSPFNGL
                     EGLKHMATAIVPPIYDASLADRDIEMPTETAYRMAKQLARTEGLLVGVSAAGAVAAAL
                     QIAEEEAAAGREAVIVTILPDSADKYLSERFWEEE"
     gene            1383..1835
                     /locus_tag="VFA02_04330"
     CDS             1383..1835
                     /locus_tag="VFA02_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZU09106.1"
                     /translation="MLKLSETIYNALRKHGEETYPHECCGVLLGRSHEGVNEVVDAVR
                     AGNTRTDSAHNRYHIAPQELVRIQRQGRERGLDIVGFYHSHPDHPAQWSKTDFAEAHW
                     LGCSYVITAVEKGAAKQTNSFLLTGTTEEDKSFEDEAIQVEELVAERC"
     gene            1917..2216
                     /locus_tag="VFA02_04335"
     CDS             1917..2216
                     /locus_tag="VFA02_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZU09107.1"
                     /translation="MKIFIPTPLRVYVNKQDAVQVDARTVGEALNALTEQHPDLKKHL
                     YSDEGKLRAFVNVYLNDEDVRYLPEKEATTVKEEDSLSIIPSIAGGCGPQERTRL"
     gene            2246..3427
                     /gene="moeB"
                     /locus_tag="VFA02_04340"
     CDS             2246..3427
                     /gene="moeB"
                     /locus_tag="VFA02_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZU09108.1"
                     /translation="MPALTEEAVAQPQLTNEEIARYSRHLILPEVGLEGQKKLKAAKV
                     LCVGTGGLGAPLAYYLAAAGIGTLGLVDFDKVDESNLQRQIIHSTKDVGRPKIDSAAE
                     KLQALNPYLKIVKHETMLTSANALDIIKDYDIIADGTDNFPTRYLVNDACVLSGKPNA
                     YASIFRFEGQASVFATEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGLLGIIQATE
                     VIKLILGKGEPLIGRLLLVDALGMKFRELKLRKNPDCPVCGTHPTVTKLIDYQQFCGI
                     APEEPKAAPVQNGIPQITPKELKQRLDAGEDIFVLDVREPHEYQIVNIGAPLIPLGEL
                     PNRISELDPNREIVIHCKSGGRSQKAAEFLKKSGFKNVVNLAGGITAWATDVDPKLPK
                     Y"
     gene            3527..>3993
                     /locus_tag="VFA02_04345"
     CDS             3527..>3993
                     /locus_tag="VFA02_04345"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZU09109.1"
                     /translation="MQETEHLDQLLTESSNPATANLDQLSTLDLIHVMHTADREVLDA
                     VGRELPNIARAVDVIVDRIDQGGRLFYLGAGTSGRLGVLDASECPPTYNTPPELVQGL
                     IAGGDVALRRSVERAEDDAEQGARDLAAHGFCPKDVLVGIAASGRTPYVLGGI"
BASE COUNT          911 a         1178 c         1142 g          762 t
ORIGIN      
        1 ttataaaaac tgggcgagcc aatggcttct ttcaattcct ctgagaagcg gtgcagggcc
       61 ttcaggtggt cagcggttgc agtaatttcc agacggctgg tcttcagaaa tttctggaag
      121 ccgcggtcga gcaggtgcga aatttcgccc cccaggacca cgcccgggcc gtccacaagg
      181 gacgctcctt tgtcactgcg cgcgagcacg gcggcgcagc cgttcttctc cacgcggtac
      241 tgtcctgcga ccccctgggc ttcgcggaca tcaaattgaa aaccgcgtag gtttgcgagg
      301 atttcgtcaa aactgggctg ctttgtcttt ttgggcatag cgatccgggc gcagcacgga
      361 ccagacttgt taaactggat acgtaagctg ccgcgtactt tcactctcgc atattttcgc
      421 ggcctcctgc aatgcatcgg ctatggcaac gatgacgcag tcactgggca ctctgcttct
      481 cgatcgcatc gggaacacac cactcgtgcg cctggagcgc attgcccccc atttgcctgg
      541 aatccagctt ctgggcaaag cagaatgggc caatccgggc ggatcggtca aggaccgggc
      601 tgcatcctcg attgtgatgg aagccatccg gaagggaaga ttaggcaaag gccagcattt
      661 gttggacgct accagcggaa acactggtat tgcctacgcc atgctgggtg cggccctggg
      721 cttcccggtc acgctctgcg tacctgcgaa tgtctcgccc gaacgaaagc gcattctgaa
      781 tgcttacggc gcccatgtcg tctggactga cccaaatgac ggctccgatg gcgcgattcg
      841 caaggcacgg gaactcgcag ccgctggacc ggaaaagtat tactacgcag accagtacgg
      901 caatgacgcc aactggcagg cccactatcg cacaacggca aatgaaatct gggagcagac
      961 cgccggacag attacgcact ttgtcgccgg actcggcacc agcggcacat ttgtcggaac
     1021 cacgcgccgt ctgaaagaat taaatccaaa gatacagtgc atctccatgc agcccgactc
     1081 gccctttaac ggtctggaag ggctgaagca catggcgacg gcgattgtgc ctccgattta
     1141 cgacgcatcg ctggccgacc gcgacataga aatgccgacc gaaaccgcct accgtatggc
     1201 caaacaactg gcgcggaccg aaggcctgct ggtcggggtt tccgccgccg gagccgtggc
     1261 cgctgcgctt cagattgcag aagaagaggc cgcagcggga cgtgaggcgg taattgtgac
     1321 catcctgccg gattcggcag acaaatatct cagcgaacgc ttctgggagg aagaataagc
     1381 caatgctgaa gctcagcgaa accatctaca atgccctgcg gaaacatggt gaagaaacct
     1441 atccgcacga gtgttgtggt gtgttgctgg gccgctccca tgagggtgtc aacgaagtcg
     1501 tggacgcggt gcgtgcaggc aatacccgca ccgattcggc gcacaaccgt tatcacattg
     1561 cgccgcagga gctggtccgc attcagcggc agggccgcga gcgcgggctg gacattgtag
     1621 ggttctacca ctcccatccg gaccatccgg cgcagtggtc caagacagat tttgctgaag
     1681 cgcactggct tggctgctcc tatgtgatta cagcggttga aaagggggca gcaaagcaga
     1741 ccaattcgtt tctgctgaca ggaacaacag aagaagacaa gtcctttgag gatgaggcca
     1801 ttcaggtgga ggaactggta gcggaacgat gttgaccgcc gggtcacccg ctcccatgcg
     1861 caggcaggcg ccgcgctgtt cccaactgaa tatgcactta cgaaaggatt tgtaccgtga
     1921 agatctttat tcctacgcca ttacgtgttt atgtcaataa gcaggacgcc gtgcaggtag
     1981 acgcccggac tgtcggcgaa gcactgaatg cgctcacaga gcagcatcct gacctgaaga
     2041 agcacctgta ctcggacgaa ggaaaactgc gcgcctttgt gaatgtctat ctgaacgacg
     2101 aagatgtgcg ctatctgccg gagaaagaag ccacgacggt gaaggaagaa gattcactct
     2161 ctatcattcc ttctatcgcc ggcgggtgcg ggccgcagga acgtacgcgc ctttgacatc
     2221 aaaattctgg agatcgaggt caaccatgcc agcactgaca gaagaagccg tggcccagcc
     2281 acaactcacc aatgaagaaa tcgcccgcta ctcgcggcac ctgatcctgc ccgaggtcgg
     2341 cctcgaaggc cagaagaaac tcaaggccgc gaaggtgctt tgcgtcggca ctggtggact
     2401 cggcgcgcct ttggcctact atcttgccgc cgcgggcatc ggcacgctcg gcctggtgga
     2461 cttcgacaaa gtagacgaga gcaacctcca gcggcagatt attcacagca ccaaagatgt
     2521 tggccggccc aagattgact cggcggcaga gaagctccag gcgttaaacc cgtacctgaa
     2581 gatcgtgaag cacgagacga tgcttaccag cgccaatgcg ctcgacatca tcaaggacta
     2641 cgacatcatc gccgacggca cggacaactt ccctacgcgc tatctcgtca acgatgcctg
     2701 cgtgctcagc ggaaagccga atgcctatgc ctccatcttc cgctttgaag ggcaagccag
     2761 cgtcttcgcc accgaggaag gcccgtgcta ccgttgcctc tatcctgaac ctccgccgcc
     2821 ggggcttgtc ccttcgtgcg cggagggcgg tgtgctcggg atcctgcccg gactgctcgg
     2881 catcattcag gcgaccgagg tcatcaagct cattctcggt aaaggcgaac ctctgatagg
     2941 ccgcctgctg ctcgtagacg ccctgggcat gaagttccgc gagctgaagc tgcgcaagaa
     3001 ccccgattgc ccggtctgcg gaacacatcc gactgtgaca aaattaattg actatcaaca
     3061 gttctgcgga atcgctcccg aagaaccaaa ggcggcccca gtgcaaaatg gcatcccaca
     3121 aattactcca aaggaactaa agcagcgcct cgacgcaggt gaggacatct ttgtcctcga
     3181 cgtgcgcgag ccgcacgaat atcagatcgt caacattggc gctccgctca ttcctctggg
     3241 cgagctgccc aaccgcatca gtgagcttga tccaaaccgg gagattgtca tccactgcaa
     3301 gtccggcgga cgcagccaga aggccgctga gtttctgaag aagagcggct tcaaaaacgt
     3361 ggtcaacctc gccggaggca tcaccgcctg ggccaccgac gtagacccca aactgccgaa
     3421 gtactaaggc cagggtgccc catcctttta cgaagtaaag ggtgggacac cacaaatctc
     3481 ctcctgtgct gtttttgttg tgcaatcacg atatgatttt ctctgcatgc aggaaacaga
     3541 acaccttgac cagctcctga ccgaatcaag caacccggcg acagcgaatc tggaccagct
     3601 ctccacgctt gatctcatcc atgtcatgca caccgcagac cgcgaggtgc tggatgcggt
     3661 gggccgcgaa ttaccgaata tcgccagggc cgtagacgtc attgtggacc gcatagacca
     3721 gggcggacgg cttttctatc tcggggctgg aacatcgggc cgcctcggcg tgctcgatgc
     3781 ctcggagtgc ccgcccacct acaacactcc tccagagctg gtgcagggcc tgattgccgg
     3841 aggagacgtg gcgctgcggc gctcagtaga acgtgccgaa gatgacgccg aacagggagc
     3901 cagagatctt gcagcgcacg gattctgccc gaaggatgtc ctggtgggaa tcgctgccag
     3961 tggccggaca ccctatgttc tgggaggcat aga
//
LOCUS       DASRBV010000111        35184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_259118, whole genome shotgun sequence.
ACCESSION   DASRBV010000111 DASRBV010000000
VERSION     DASRBV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 35184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35184
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259118"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFA02_04350"
     CDS             <1..917
                     /locus_tag="VFA02_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU09110.1"
                     /translation="RSAGAGYNSKGRLLPLGVHAPMSGPGLALGAAAVWGASDFCGGL
                     VTRRASSALIVTIAHGLGLLLLLLALIVLHPGAPSQQTTIYGLLAGLAGGVGLLFLYK
                     GLSLGSMGLVAALSGVLTAVVPVVASLFSYGHPTALQSMGFAVAIVAIWLIAYAPGSG
                     AHPHGLGFGAASGICFGFLLYFLHLGGKESLLWALTFSRIGSVTCAVAFAVYIAWRGT
                     LPLRNPGVRWAAILPLAAVAGLLDVSGNLLYTASSLAGRLDVAAVLSSLYPAGTILLA
                     IWLLKERATRSQATGMALAVLAVAMISA"
     gene            1046..1252
                     /locus_tag="VFA02_04355"
     CDS             1046..1252
                     /locus_tag="VFA02_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09111.1"
                     /translation="MLNAQTPERGQLLAALERDLAAKKDQLNMLISMFHALPRMSRSY
                     IIPVFRSTRREIATLRRQIRSLRH"
     gene            1512..2327
                     /locus_tag="VFA02_04360"
     CDS             1512..2327
                     /locus_tag="VFA02_04360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZU09112.1"
                     /translation="MPRTAGIMSFPDFRGFTRSLILWNVGIYFLLLLLGVVATPVAQA
                     IVRVGALIPLYFLHGYLWQIITYCFIHTGILGTALEMLTLWFLGSFLESNHGARWLAE
                     LFFISVIGAGLAAIGLSLVAGANGFSLFAITGCFGGIFGLLVAFGVLYGDLEFMLFPF
                     PMTMKAKYLVIVYMLIALAMLFSSSRIYAFAELGGALAGYLFIKAAPRRGYAFAMSEG
                     YFGLRNSYYRWKRRRAAKKFEVYMRSQNRDAYFDKEGRYTGSEKDPNDRKWMN"
     gene            2426..3136
                     /locus_tag="VFA02_04365"
     CDS             2426..3136
                     /locus_tag="VFA02_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZU09113.1"
                     /translation="MMLASTWFLALPARAEDGPDDASAALIVTADFNHDGLPDFAKVA
                     APEKDHPGPAHLVILLGQADGPPVEVASLVAPGPAPTSIAAGDFNKDGHPDILLGDST
                     GALMLFQGDGTGRMAPAGTVAHFDSVVSIAVADFNHDGIPDIAVSDWRASRATVLLGD
                     GKGGFGPTWSFPLRMRGKAPHLAAADFNGDGIPDLAVVYDDDDGDTYDVMLNNGNGTF
                     SAAPDLGFVKDPNAHCVT"
     gene            3534..5345
                     /gene="lepA"
                     /locus_tag="VFA02_04370"
     CDS             3534..5345
                     /gene="lepA"
                     /locus_tag="VFA02_04370"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZU09114.1"
                     /translation="MDSKFIRNFAIIAHIDHGKSTLSDRLLELTGALTAREMQAQVLD
                     AMDLERERGITIKAHAVRMMYPAKDGQTYQLNLIDTPGHVDFSYEVSRSLASCEGALL
                     VVDASQGVEAQTLANAYLAINHGLEIIPVINKIDLPSADIPRTKEMVEQTVGLDGNDA
                     IPVSAKTGQGVPDILEAIVHRLPPPKGDPEAPLQALIFDSWFDAYRGVIVLARVVNGT
                     LRKGAKIRLMSNNKVFTVESLGILTPKPVEIDQLSAGEVGFFVATIKNVSDTKIGDTV
                     TEDDHPADTPLPGFEDIKPMVFAGLYTVDSHEHTLLRDALEKLRLNDSSFFFEPESSA
                     ALGFGFRCGFLGLLHMEIIQERLEREFNLDLITTAPSVRYKVTMTDGTLLEVDNPSRW
                     PDPSEIEKVEEPIITATILTNEEYVGGILKLVEEKRGRQKNFEYVSPTRVMLTYELPL
                     NEIVLDFYDRLKSVSRGYASLDYHLSGTWESPMVKMDILVSGEPVDALSIIVHRDHAY
                     ERGRALVSKMRELIPRQQFEVAIQAAIGAKIIARETVSALRKNVLAKCYGGDISRKRK
                     LLEKQKEGKKRMKRIGKVDIPQEAFLAVLKVGEESSR"
     gene            complement(5741..6409)
                     /locus_tag="VFA02_04375"
     CDS             complement(5741..6409)
                     /locus_tag="VFA02_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZU09115.1"
                     /translation="MKRSLAIVCMLASGLGLSAMAQAGATDPAPGAAPAVAPAGGTKI
                     AIIAFQPAVMQTNEGQRNFSELQKKYEPKRQQLKQMSDEIDGLKKQLQSAGNNLSDTE
                     RQNRLRTIDDKEKEYQRLGEDTQNDFQQDLQQTYQQLAEKVYNVLQAYAQQNGYSVVI
                     DSSAQSSPVLWASEATNITNAVIQAYNQKSGVPAPAAGSAASAPSAPAPHTTAHPTTT
                     APKK"
     gene            complement(6673..6945)
                     /locus_tag="VFA02_04380"
     CDS             complement(6673..6945)
                     /locus_tag="VFA02_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZU09116.1"
                     /translation="MAWTIKFDPSAEKELDKLDPQIARRILKFLFQRLAILEDPRSIG
                     EALHGPRFGELWKYRVGDYRLICSLQDEVLTILVLRIGHRREVYER"
     gene            complement(6929..7153)
                     /locus_tag="VFA02_04385"
     CDS             complement(6929..7153)
                     /locus_tag="VFA02_04385"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HZU09117.1"
                     /translation="MLAIRLPEEIEARLDKLARSTGRTKSYYVREAILHHLDELEDIY
                     EAEKRLEAIKAGKTKLVPVEKIMKRYGLDH"
     gene            7296..8051
                     /locus_tag="VFA02_04390"
     CDS             7296..8051
                     /locus_tag="VFA02_04390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZU09118.1"
                     /translation="MSPARKLQMEPGDPEQENVSAATRKMRMLRSLVCGNKYEKAARA
                     QGATLVAGVDEVGRGSLFGCVVAAAVILPENTRIRGLRDSKQLLREDRERLARIVEQK
                     AIAIAVEEVDVETIDRINIYQASRLAMMGAVARLAPQPDHLLIDALHLDLPCAQTSII
                     YGDSLSISIAAASVIAKVYRDQRMRELHEIYPQYGLASHKGYSTPEHLAALEKYGPTP
                     LHRKSFRPVSQTTLPWDMPLEDTIAEVPAIQVE"
     gene            8392..9009
                     /locus_tag="VFA02_04395"
     CDS             8392..9009
                     /locus_tag="VFA02_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU09119.1"
                     /translation="MPHTPEQVETLKHAPAADGVADVIYARWSPRAYADKQVSSEDLR
                     KIFDAARWAASSYNEQPWRFFVGRRGDATYQKIFDTLVEFNQQWAKSAPVLILSVASK
                     KFAHNGSPNYHALHDTGAATATLSLAATSLGLHTHSMAGFDHDKARKAFNVPEDYDIG
                     AVTALGYLGDPEGLPEQMKKTELSPRQRKDLGQFVFSEWERPAVL"
     gene            9021..10349
                     /locus_tag="VFA02_04400"
     CDS             9021..10349
                     /locus_tag="VFA02_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZU09120.1"
                     /translation="MSASITAQERRERGRLHRKQVRRQDHCLWYPKERRHDPVALLEE
                     SMRGRVPSLIALKYERMAASPFGFFRGAVPVMAADLSLLPHTSIINQICGDAHVRNLG
                     AYAAPDGRLVFDINDFDETIRGPFEWDLKRLATSLVLAGREVGDKRAVCEEAVHRLVF
                     QYRKYADLFARMPVLDMVRFQVHRLQRISPVSAALLKAERATPVHALEQLTVPAKGRA
                     GEEGKRVFKEEKPVLTRLSAAQARAVLASLQEYKQALLPERRHFFAQYRPVDVGFKVV
                     GTGSVGLRDYLVYMEGNGGGDPLFLQIKEEPASAYAKYLEYHGPHHQGQRVAEGQRAM
                     QFQSDPFLGWTTIGHRHYLVRQLNDHKASIEIEDLKGQGLLEYAEMCGELLARGHSRS
                     GDPCLIAGYLGNGKRFAEAILKFAEAYADQTERDWEALRRSKHMGRNRNA"
     gene            complement(10390..11619)
                     /locus_tag="VFA02_04405"
     CDS             complement(10390..11619)
                     /locus_tag="VFA02_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZU09121.1"
                     /translation="MKFFKTAVFCLSLLCASPGPALYAQRGFVHTKGTELVDDQGRPL
                     MLRGINLGNWFEPEGYMFHFDGGPQSPREIEELSYELIGPEKADAFWKQWRETYITQS
                     DIDRIRESGFNSVRVPLHWKFFDSENAEGFRLLDRLVTWARRDGIYLILDLHCAPGGQ
                     TGSNIDDSWGYPWLYRSAAAQAHTAAIWRRIAAHYRNEPVVLGYDLLNEPVPHYPRLQ
                     QYNPDLEPVYRKLVAAVRQVDKNHVVILGGAQWDSNFTVFGPPFDSNVMYTFHKYWTA
                     TDKSVIQEYLDFRDKYHVPIWLGESGENKDEWIAAFVKTLEANRVGWCFWPYKKMDAT
                     SSVVTFDRPAHWDDIISFAKLPTGTGNAEKRIAARPSVEEAQEAFDDLLKKIQFAHTR
                     VNGGYVKALGLTVAGPR"
     gene            11945..12214
                     /locus_tag="VFA02_04410"
     CDS             11945..12214
                     /locus_tag="VFA02_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09122.1"
                     /translation="MKFRLIFVLFCAVELFVLLIAFEQPAHAYVDPGSGLLIFQVAGS
                     MLTGACFMLRRKLRRLFGHWGQQNPQQENQVNSDEAQTCGANSSR"
     gene            12195..13580
                     /locus_tag="VFA02_04415"
     CDS             12195..13580
                     /locus_tag="VFA02_04415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU09123.1"
                     /translation="MQTPLADVSRIATFRDPAGSLRVENGRVLRTLRHEYADTVLKFI
                     QSKTGEEWVNSGRLVRTTVLPEQEGAGEHLLEHPHIFFPSYPWEWTPGQWAAAAELTL
                     DLCEALLDHRLILKDATPLNVLFDGPQPVFVDVSSVEQRDVSSPIWAAWAQFVRTFLL
                     PLAAYKYLGWPLAASLQKRDGYEPADLYPHLSLWQRWSSPLCSLVTLPYLLEKRKGAA
                     SRMPKQSPEIATAVLRRNLRLLRKMVRSLSPGKYGSRWSAYPENATHYSAEDHSVKQK
                     FVCNSLLKMRPSAVLDLGANTGTYSRIAAGLGARVVALDSDAQALERNWRDAQAQKLS
                     IQPVVADVARPTPAVGWRNAESLSLLDRARGRFDCVMALGLMHHLLLSDQVPLREISR
                     LMRDLTQRWLIVEWVPAADPKFQELLRGRDALYAHLTEETFLDAFGHDFARVLREPLK
                     NGRALFLLERR"
     gene            complement(13866..15506)
                     /locus_tag="VFA02_04420"
     CDS             complement(13866..15506)
                     /locus_tag="VFA02_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HZU09124.1"
                     /translation="MRIGLRLYSKYMAIVACAVLALICWACPLTLQAQAPQVAYTVTS
                     AWGSGFQTSVTITNTGSTPIQNWKLQFTLPYAISSIWNAQVAAQSENTYTIIGDSWDI
                     SIPAGGSVNFGFIGSAYAGNAPQNPANCLVNGEAVSSTTCTTGSGTVPPGAPTGLTAA
                     DVTSSSVTLSWTAAAKGTNPITSYNVYENGKLLTSSTTTSTKITGLTASTTYQFTVAA
                     VDSAGVISSQSSAITVTTSGGSGNSGGGADGAFPSHFFAPYVDVTVYPTFNLTQSAPS
                     AGPYFTMAFIVDGGGCSASWGGVVPLSQNWMLSDVQNLRAGGGDVIVSFGGEAGSELA
                     QTCSTEAALQAQYQAVIDMYQLKRIDFDIEGAAEADTTSIGLRDEAIAALQAANPGLE
                     VSFTLPVLPTGLTQDGLNVIQDAISKGVKLKTVNAMAMDYGQPDSQMGQDAINAGTAT
                     AKQLQSLYPELSSSQALAMVGVTPMIGVNDTQGETFSLNDAQLLLNWARSNGVGFLSM
                     WSATRDSACVDGSTSAEPTCSGVSQQPFAFSEIFKQID"
     gene            complement(15804..17138)
                     /locus_tag="VFA02_04425"
     CDS             complement(15804..17138)
                     /locus_tag="VFA02_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZU09125.1"
                     /translation="MFRPVAQRFLKRSVLGLVILAGSAGIAVAQVPAGFGTTPIFDEE
                     FNGSSLDTSVWSYRGEGTQRDDCFIDSSAVHVANGYARISIYTANNAQGVPTNYCGAI
                     TTQNGSFLHAYGYWEANVRYQYQQGIQCSFWIQSPTVGATIGNPQQSGVEMDVFEHTH
                     FNATPTGYDHAVIWNGYGAYSQSASHVGTLSNLNDGNFHRFGLAWTPGNLTFYVDGQQ
                     TWSLNASDAAISDIAEYIILDTELPNASYVPAGGYGPLGSSTNAYLDVDYVRIYPYST
                     TVTSTTLSDIADAYVRDGSYANTNFGGSSTLVVKQDATGYNRNSWLKFDLSQITGTVL
                     QATLYLTPTSAGENNIYNFANYATDNSWIADEITWNNQPKVSGRLSTGISFGAGILTY
                     FDVTAAAKAGQQLSIQIAPDTPTGSSAWVEYASQENGTVNYQPRLVVLSSAP"
     gene            complement(17330..18583)
                     /locus_tag="VFA02_04430"
     CDS             complement(17330..18583)
                     /locus_tag="VFA02_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09126.1"
                     /translation="MKAGSLGNDSLSREAWIPSSAEDQALIYNMLEEILASAPFRSSR
                     RYPAMLRYIVEKTLEGQQECLKERTIGVEVFGRDPGYDTNADPVVRFSAGEVRKRLAQ
                     LYHDTSKDYPVEVELPLGTYIPRFHRRAVSVLPGEGPALDSGLPANGPSPEPAVPYAG
                     KRRMVWPVYLILALLIATGIGGIYAYKKLQTPDPVMAVWTPLLKISGPVLISAGRPHQ
                     DMEPPELPNISIKDHILRPEFRVSITTVSAIANIVGFLQAHQKPFRIHEAYSNTLADL
                     HDRPVVLVNGNNNKWTLLLLRPLRFHFVQEGDFAYIEDAQHPAYRGWNVDFSKPYFGQ
                     TTDYAIVARFDSATTGGPVIVVAGISSNGTEAAGEFIVSPEKLAELEKSAPPGWASRN
                     FEAVLKVEVVGGNTGAATVVASQFW"
     gene            19038..22706
                     /locus_tag="VFA02_04435"
     CDS             19038..22706
                     /locus_tag="VFA02_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU09127.1"
                     /translation="MTFAQSGAGAIQGTITDSTGAVIPNAAVHVVNQGTGQSFDSTAN
                     TAGFYSVPALFAGNYTVTFTSPGMAKYETSIALQAAQTAVISPILTPGAVQQQVTVTA
                     NTIQLATYDSGTISSQLDNNRINQLPMNGRNVLTLTGMTTPGLESGGTRANGNMQAAI
                     EYVQDGAPLSDRNFGGPTIMPDPDSIQEVKVETVASNARFATPATAIVTTKSGTNQLH
                     GTFFETARNNAIGIAKSRQNAPNFAAPHLVRNEFGASIGGPIFLPKLYDGKNKSFFFF
                     AYERYSLRSEQDELVTVPTQAMRNGDFSGLINSAGQLQVLYDSNTTNPVTFQRQPFAN
                     NQIPMGRLSPLAKTLYSISPLPTSADNPMVSSNFRAPGINNSTVPNMTARFDHTFGSQ
                     NSAYLRFTSTQSTTLGLRNYPTNQADTIGGSGLPAGASNEGLTKVSVYSAGLGYMHVF
                     SPSFFSETVLSNEWESGHYGAGGNPSLNYEAMLGLPNNFGSTGFPQIGSTSLFMNYAG
                     TQFNYASSQILTNLDENLSKTIGRHQLFFGGRYRHERLGALPDRTPDALDFNGMGSAN
                     VNPASGKSYTALPNTGDANADLFLGAAYYYTIRLNPPYEHWRNQEFDSYFQDNFQVNS
                     KLTISGGLRWEIHPTATEKNNVFNGFNFKDKAIVLGQPVSRFIAKGFTTQAIITNLQN
                     LGVTFESPQQAGLPEHMVYSNNFVFSPRIGFAYAPFGSGRGTVIRSGFGRYAYPIPIR
                     NFYSVGKLNAPFATAYTQSYTAANQSPDGQPNYLLRSPQTVVAGQNSSNIVNSNQTNS
                     ILPGGPGEVVLNPHYPPNFVTQFNFTVEQPLKLDSVLRMSYIWDHGSNLDQLNNHNNA
                     MSSYVWQVTTGTLPPTGAYASVALNPYDNVTYGQLTTVDRTGWSNDNALQLNYQRLYK
                     KGYAYQIYYVFSRAMRVGGNTFRDSNIYPYADYAPGAAPSSDFDKLNRFQNYQIDSAT
                     PKHRISWNGIVDLPFGHGKTFFGNAGRLVDELIGGYQIAFDGTVFSQYFQPSSSYWGG
                     VNPQGAGSIGPLHVYKKKYKITDCSSGVCRPGYLWFNGFINPLQIHNPCGPNAYTGIP
                     SNYTPYQTPINMDPGTVTCVNGAVKTSNSNYLTNNVPVKLANGSTVQTAYNPGPAGVN
                     PFSHTFLSGPFNWTADLSLFKVFPITEKINLRVNVDAFNAFNVQGYTNPSPSTGIQTF
                     LSSYNTPRQLQFTARLTF"
     gene            22769..24193
                     /locus_tag="VFA02_04440"
     CDS             22769..24193
                     /locus_tag="VFA02_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09128.1"
                     /translation="MPFISRRALISRGMTVAAIGALCRSSSWARVIGEDTSPVHLSVS
                     VDWENVISSVSPEMLGLSYESSQLSAPDFFSAANTELIALFKKLSPQGVLRLGGNLSE
                     YTVWVSSRTEEKPRIPFRAVGADSGAKKAAMPSPVSVQAIRNLRDFLESTGWRAVYGL
                     NLAQADHARVIDEAVFVADTLRARLIAFQIGNEPDHYVMNGLRAPGYGFQDYFAEWSR
                     IYDAVRKAVPSAQFAGPDIAEDVAWVDAFAKAAPSSVAFLSGHHYAEGPPTDPTMTLE
                     RLLSPDPSFNHRLAVTEKIAQEARIPYIMAETNSCYNAGKAGVSDVFGSALWAMDYLL
                     QLAQAGTRGVYFHGGANSWYTPIAGGSGQPFYARPVYYGLLMCRDLLGSELVSVKVKA
                     DAANVSIYAVQRPKRLVVINKGPGNFTIELNRGECSEQVLRLLAPSPEAKTGIKVDLQ
                     SFHATEKRLFVPKYSATIFDIQSS"
     gene            complement(24500..25522)
                     /locus_tag="VFA02_04445"
     CDS             complement(24500..25522)
                     /locus_tag="VFA02_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU09129.1"
                     /translation="MAHTMKEVAKLAGVSPATVSRVLNKTHYIAAATEKRVLEAVSQL
                     NYYKNVHAKRLSTGQSDLFGLVISEIANPYFPEIIRGFQAAAWDQGFDVLLCNTEYSP
                     RRTQSVINKLIQSDVRGVAIVTSSIDNSMTPDLIEAGIGVVFCNLCPPGKLVSNITID
                     YQHGVSQAIKHVAALGHHRAAVIAGPETNRTAANIKQALLSGLKRRGIRPFPVTSTDY
                     RLDAGASAVKAILSAPEIPTVIFCGSDLIAMGAMNALEEAGVKIPEDISIVGIDDISF
                     AFLARPPLTTISVPRERLGITAFHALDKMLKLKRRIGADYRLETELVVRKSTAPARQR
                     NLQISR"
     gene            25859..29140
                     /locus_tag="VFA02_04450"
     CDS             25859..29140
                     /locus_tag="VFA02_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09130.1"
                     /translation="MIEKRNKCWYWLLVAVSLIPTRLAAQAVGSIGGTVTDPTGAVIA
                     QARVTATREDTKVSQSTLTNESGTFVIPNLTVGTYTVQVSAGGFTTRSITGISLDVSQ
                     QRDIDFRLTTAGTVETAEVTAAPPLINTSDGSLAGLVSEKQVESLPLNGRSIQNLVML
                     QPGMAQDSGSMGWLAPQWISNGNRGETEVATLDGSDATDTEMGTVQFWNFNLDAIAEF
                     KVQQANYSAEFGQGGGTITQIVSKSGTNQFHGSAFEFIRNSVFDAANYFSSSGVSPFQ
                     RNEFGATIGGPILRNRTFFFGEYAGFRQRYGTTTILEVPTADQRNGLVTINGFEYKVP
                     LNPVAQQILNKYPMPNQPAGIYGENTYNTLFKQPTDDDQFSVRIDHKISDKDSIFGRA
                     SYINNSQKAVDPTAAVEDPAFSAENINNPRNYAVSETHIFSPKLINNFTFSLNRQIEG
                     STPLSQQYTQTTTGDGTLSNWGPDTFITKYVETYYITSDKVDWNWGRQFFTIGGDYRY
                     GQDNGFGVTSAGPNGQYTFTAGTPLTVDIPSTNGGPTIPAGTGSPSGLISIMAGDAQS
                     YKRSTTIPGFGPAGGGGVHWGLRRWHIAPFVQDDIKFTPKLTVNLGLRYEYNSVPYEI
                     ENRLGGIADTGSLYGHFVLNPQPLYQPDRLSFAPRFGIAYRAKDKTVLRGGVAIFTNV
                     IPMVYPDQAAVNFPLASYSSLSNPPYSLTPLPVSLPTLTSTSGTVMPPNGGPKKIPPN
                     TPVNLAPIAAETGPIIGDWPSDKMKNGYTLTGNFTIEQQMPGDIALQMSFITNNGISL
                     YQSSYPNAFTGAQTANTPFTNVTPGLGEVEVFYNDAISHYNAMQLQMRKISPLHGLQY
                     QINYTWGKNLTDADAVWSAPGTSGGVTQNDPTCIRCEYARASYDLRQRFVANFSYNIP
                     GSWGLVPSPISKGWQVLGIYSAQSGFPFTIVGPYGTLQYGFDTLNGVGARPFFIKWAQ
                     RDPQHRAQFFTSDAINNTSAYFNEPTVTSNISGVGAVQTSPGNLGRNTYTGPGWWNMD
                     FSVLKDTRLTEVLNMQFRAEFFNIFNHPTFGTPGGTIGSSNFGLSTSTQSSERELQFG
                     LRFMF"
     gene            29280..30650
                     /locus_tag="VFA02_04455"
     CDS             29280..30650
                     /locus_tag="VFA02_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU09131.1"
                     /translation="MNKALFSRREFLQTTAGAAGASLISGANFLNAEPIPSASQPVAP
                     SDQVRFGIVGVGMEGSGLLSTAIQLRGVECVAAADLYDGRHQLAKEIVGKPIRTTRRY
                     QELLDAKDIDAIIVAVPDHWHKQVVVDALAAGKDVYCEKPMSHNPADGLAMVAAAKKT
                     DRIVQIGAQRTSSVLCAKAKELYEKGAIGELNLVEATYGRNDPNGAWEYPPPPDLSPE
                     NLDWDTWLGTAPKIPFDPNRFARWRCWKEYGTGVAGDLLVHLISGMQFVLGINEYPKR
                     VSAFGGIYHWKDGRNMPDVHPVLFEYGNGNSNIPVYMRLSLACESTEVTRFQGPKGAI
                     ELREFSVTYIPQAGVDLSPSYYTASFPAAMRNAYVKQWHEEHDPVPGQEPAPESITFN
                     GNDYDDLKPHLWKFFEAVRSRKPVVQDAVFGHNAALACHLANESYFRKSPVYWDPASE
                     TIKSLE"
     gene            30735..31700
                     /locus_tag="VFA02_04460"
     CDS             30735..31700
                     /locus_tag="VFA02_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZU09132.1"
                     /translation="MKLQRVGFITITAIALIFSPTFIHSQATGSSAASAAPFLGRWDL
                     TLKAPDKEYPSWLEIRQEDGRLKARMTGRWGNARPLPTVEVSNGELTFVSPKEEEGSK
                     TDLVFQGKLTGKTLTGTVNGPDGKTWQWVGVRAPALERTDTPKWGKPIQLFNGKDLTG
                     WKMSAPGPPVWTVQNGNLVSPGNGPELISDAKFQDFKLHLEFNCGKDSNSGVYLRGRY
                     EVQIETESEAEPPSHHTGGVYGFLAPNPEQPRTPDVWQTFDITLIGRKVTVVLNGKTV
                     ISNQEIPGITGGAIDSHEAQPGPIYLQGSEKGHVAFRNILITPAQ"
     gene            31777..32871
                     /locus_tag="VFA02_04465"
     CDS             31777..32871
                     /locus_tag="VFA02_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZU09133.1"
                     /translation="MTRFLQDILRQPDELLRVIDHLNKAEQKLLESAAGAIRGARHVY
                     ITGIGASWNAALGAGYIFHAGGWPVYMLDASELLLFAEIPPDAVILVLSRSGRSIEIV
                     KLLTKARAAGAAVIGVTNFEDGPLAQEADIPVIIPVKADHGISVNTYSALAAAAAAIA
                     NATVSSFGEELIAALSSSVAETAKRIPEWRQQLEKTSWLLPGAPYYFLARGASLASAY
                     EAGLLWEEGAKLPATAMGTGSFRHGPQEVMMKDVRIAIWIDGEQMREQDLAVANDLRD
                     LGASVMLTGYDLPEDAGDLVFQMAPSSRHWQFLTDIIPAQLAAERLAQVAGVDCDSFR
                     FASYIVEDDFGLLPNGATSLAKTGSNGNET"
     gene            32883..33827
                     /locus_tag="VFA02_04470"
     CDS             32883..33827
                     /locus_tag="VFA02_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZU09134.1"
                     /translation="MNAIGAVDIGGTKVAVGIVNEDGRLISRMEAPSRSVEVYSDGAR
                     LITDMLRQSTQATGQAIVGIGIGATGPVNPFTGRFGDLDFLPQWSGESLVDDLERDFR
                     VPVALENDADAAALAEAGWGAGRSKSRLIYVTVGTGIGGGIILDGRLYRGVDGAHPEI
                     GHQVIDTGGPLCSCGLNGCWEALAAGPAMVRWMESQPEYFSSEELTAKRICELAYVGN
                     ALALRAIEREIFYLGIGLANLVNVFTPDALILSGSVMKSADLFLNGIRKVMRQGCRFV
                     PLEKVKLTLASLGEDANLIGAARAWHERFAHGEKHVCQ"
     gene            33909..35156
                     /locus_tag="VFA02_04475"
     CDS             33909..35156
                     /locus_tag="VFA02_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU09135.1"
                     /translation="MVYLVLLTFFVISLLTNILGPIVPDIISNFHVSLTAAGFLVFSF
                     FIAYGVMSIPAGFLVERFREKPVMISAFVSAAVGSMSFAFFPEYKVAVVSLFVIGAGM
                     ASLQVAINPLLRVSGGEEHFAFNEVLAQLLFGTASFISPHIYSYLVLNLHRNPQGSNL
                     LVRGLNRVTPIALPWVSLYWIFSAIAVAMVLVLYLARYPRVQHTDEERPGTWQMYGCL
                     IRRRLVWLYFFAIFAYVGCEQGSANWISQFLTQYHGLDPHTTGARAVSWFWGLMTAGC
                     LAGVLLLKIFDSRRVLIGASAGALCCLSLALFGSGRISVVAFPLVGLFASVMWPILIS
                     LALNSVSEYHGSFTGILGTGIMGGAVMPAIIGRIGDHFGLRNGLSLLYLTFGFIFSVG
                     FWAKPLVNNATLRWKSTDLSAVV"
BASE COUNT         7867 a         9414 c         9662 g         8241 t
ORIGIN      
        1 gtcgaagcgc cggggctgga tataacagca aaggacggct actacccctc ggcgtccatg
       61 cgccaatgag cggtcccggt cttgccctgg gcgctgccgc cgtctggggc gcatccgatt
      121 tctgcggtgg actggtcacg cgcagggcgt cctctgccct catcgtcacg attgcccacg
      181 gtctcggcct gcttctgctt ctgttggcct tgattgtctt gcatcctgga gcgccgtcac
      241 agcaaaccac tatttacggt cttcttgcgg gcctggcagg aggtgtaggc ctgcttttcc
      301 tctataaagg actctcgctt ggctcgatgg ggctggtggc ggcgctctcc ggcgtcctga
      361 ccgctgttgt gcccgtcgtc gcatccttgt tcagttacgg acaccctacg gccctgcaat
      421 ccatggggtt cgccgtcgcc attgtcgcca tctggctgat tgcctatgcg cccgggtcag
      481 gggcgcatcc gcatggtctg ggttttgggg cagcctccgg aatctgcttc gggtttcttc
      541 tctacttcct tcatcttggg gggaaagaaa gtcttctttg ggcgttgaca ttttcccgta
      601 tcggatcggt cacctgcgct gtggcgtttg ccgtctatat agcatggcgt ggaacgcttc
      661 ctttacgaaa tcccggtgtc cggtgggcag ccattctgcc gttggccgct gtcgccggac
      721 tgctggacgt ctccggcaac ctgctttata cggcctcttc gctggccgga cggctggatg
      781 tggccgcggt gctgtcttcg ctttaccctg ccgggacgat tctgctggcc atctggctgc
      841 tgaaagaacg ggccacccgg agtcaggcga caggaatggc gcttgccgtg cttgccgtgg
      901 cgatgatttc ggcttaggct tatattcagt taattttaag caatctctgg atcgtgctgt
      961 ctttggttcc aatttgtaac cggccagaat acaatcagca ttttgcggga tgacaatgcg
     1021 cctccattat ggttaccatt ggataatgct caacgctcag accccggagc gcggtcagtt
     1081 gcttgccgca ttggaaagag acctggcagc gaaaaaagac cagttgaata tgctcatctc
     1141 tatgttccat gcgcttccac gcatgtcccg ctcctatatc attccggtgt ttcgcagcac
     1201 ccgccgcgaa attgccaccc ttcggcggca aattcgcagt cttcgccact agagcatttt
     1261 ttgtcattcc tggcggcggg gaaaactctt acatcgccgt aattctttct ccctacggtc
     1321 gtccgcagca tggcaccatg cggctgccat gaagccccgt ccgggatcag gcgcaagctc
     1381 cttcctgagg aaaatcagca aaccacgccg caaaactttt tatgtcagtg cgcgtaactg
     1441 taatggaagt ggtcatcacg gaagaactat aactgtacga caaaccgcgc gattcctgag
     1501 aaaatatcca tatgccacgt actgccggaa tcatgagctt tcccgatttc cgcggcttca
     1561 cgcgcagcct gattctctgg aacgtcggaa tttattttct cctgctgctg ctcggggtag
     1621 tggccacgcc tgtggcccaa gccattgtcc gcgtaggggc actgattccg ctctactttc
     1681 tccacgggta tttgtggcag atcatcacct attgcttcat tcacaccggg atcctgggta
     1741 ccgcgctcga aatgctcacg ctctggttcc tgggcagctt tcttgaatcc aaccacggcg
     1801 cgcgctggct cgccgaactc ttcttcatca gcgtgatcgg cgcggggctt gccgccattg
     1861 gactcagcct ggttgccggc gccaacggct tttcactgtt tgccatcacc ggatgctttg
     1921 gcggaatctt cggactgctg gttgcttttg gcgtgctcta tggcgacctt gagttcatgc
     1981 tttttccctt tcccatgacg atgaaggcca aatatctcgt catcgtctat atgctgatcg
     2041 ccctcgccat gctgttctcc tccagccgta tctatgcctt cgccgagctg ggcggggccc
     2101 tggctggata tcttttcatc aaggcggccc cgcgccgcgg atacgctttt gccatgagcg
     2161 aaggctactt tggtctgcgc aacagctact accgctggaa gcgccgccgc gccgcgaaaa
     2221 agttcgaggt ctacatgcgc agccagaacc gcgatgccta cttcgacaaa gaaggccgct
     2281 acaccggctc agaaaaagac cccaacgacc gcaaatggat gaactaaggc cgcaattcgt
     2341 ccgcacgccc gggcaaaatt cacgcctaga accagtccgc ttctgccgcg catggcgccc
     2401 gctccttgct gccgtgatga ttggaatgat gctcgcaagc acctggtttc tggctctgcc
     2461 tgccagggca gaggacggcc cggacgatgc ttctgccgcg ctgattgtga cggccgattt
     2521 taaccatgac ggtctgccgg atttcgccaa agtcgccgct ccggagaaag accaccccgg
     2581 ccctgcgcat ctggtcattc tgttgggaca agctgacggt cctcctgtag aagttgcctc
     2641 ccttgttgct cctggccctg ctcccacttc catcgctgca ggcgacttca acaaagatgg
     2701 ccacccggac atcctgctag gggacagcac gggcgcgctg atgctctttc aaggcgatgg
     2761 aacaggcagg atggcccccg ctggcacggt cgctcatttt gattccgtgg tctccattgc
     2821 agtggccgac tttaaccacg acggtattcc tgacatagca gtttccgact ggcgggccag
     2881 ccgcgccaca gtccttctgg gagatggcaa aggcggattc ggccctacct ggtctttccc
     2941 gctccggatg cgaggaaagg ccccgcatct tgccgcagcc gatttcaacg gagacggcat
     3001 ccccgacctg gccgtcgttt acgacgacga tgacggagat acctacgacg tgatgctgaa
     3061 caatggcaac ggcaccttct cagccgcccc ggatctgggc ttcgttaaag atccgaatgc
     3121 ccactgcgtc acctaaattt tcgtttcaat gggaaaggat ggtcgaaaat cttagcagga
     3181 tgcgcccatc tcacacatcg ctaagattct tcctgccttc ggtggttaga atgacagggt
     3241 ttatatatcg gccgtcagaa tggcgaggtt tatatattca gtcgccagaa gtacgggcta
     3301 tatatctgtc gtcaggatga tggactctgc atatacgaaa tgcttgtagc gcttagtatg
     3361 gtttgttcgg gctttgcctg aatgccccgc tccttttctc tgcgataata aaaccacagg
     3421 tttggaaagt cttgaaaaac gggtttttcc actcaggcaa aagaggcttg cgtgggtcac
     3481 cccaggtctc ttaaaggatg aaagggcctg gcttccgcct cacaagtttt cccatggaca
     3541 gcaagtttat tcgcaatttc gcgatcatcg cgcacattga tcatggcaag agtacgctct
     3601 cagaccgtct gctggagctg accggagcgc ttactgcgcg tgagatgcag gcgcaggtgc
     3661 tcgatgccat ggaccttgaa cgcgagcgtg gcatcacgat caaggcccat gccgtgcgca
     3721 tgatgtatcc ggcaaaagac gggcagacgt atcaactgaa cctgattgac acgcccggac
     3781 acgtggattt ttcctacgaa gtctcacgct cgcttgcctc gtgcgagggt gctctgctgg
     3841 tggtggacgc ctcgcagggc gtggaggcac agacgctcgc aaatgcgtat ctggccatca
     3901 atcatgggct cgaaatcatt cccgtcatca acaagattga cttgcccagc gccgatattc
     3961 cgcgcacgaa agagatggtt gagcagacgg tcggcctgga tggcaacgac gccatcccgg
     4021 tgagcgccaa gacgggccag ggcgtgcccg acattctgga agcgattgtg caccgcctgc
     4081 ctccgccgaa gggcgacccc gaggccccgc ttcaggcgct gatttttgac tcgtggtttg
     4141 acgcttatcg cggtgtgatt gtgctggcgc gcgtggtcaa tggcacgctg cgcaagggcg
     4201 caaagatccg tctgatgtca aataataagg tctttacggt ggagtcgctt ggcatcctga
     4261 cgccaaagcc ggttgaaata gaccagcttt ctgcgggaga agtcggcttc ttcgtcgcca
     4321 cgattaagaa cgtcagcgac acgaagatcg gcgatacggt gaccgaagac gaccacccgg
     4381 cggacactcc gctgcccggc tttgaagaca tcaagccgat ggtctttgcc ggtctttaca
     4441 cagtggattc gcacgagcat acgctgctgc gcgatgctct ggaaaagctg cggctgaacg
     4501 attcgtcgtt cttcttcgag ccggaaagct cggcggcgct gggttttggt ttccgctgcg
     4561 gctttctggg tctgctgcat atggagatca tccaggagcg gctggagcgg gagttcaacc
     4621 tggacctgat taccacagcc ccaagcgtgc gctacaaagt cacaatgacc gacggcacgc
     4681 tgcttgaggt ggacaatccc tcgcgctggc ctgatccgag cgagattgag aaggtcgagg
     4741 agccgatcat cacggcgacc attctgacga acgaggagta tgtcgggggg atactgaagc
     4801 tggtggaaga gaagcgaggg cggcagaaga acttcgagta tgtctcaccg acgcgggtga
     4861 tgctgactta tgagcttccg ctgaatgaga ttgtgcttga tttctacgac cggctgaagt
     4921 cggtctcgcg cggatacgcc tctctggatt accacctttc cggcacctgg gaatcgccga
     4981 tggtgaagat ggacatcctt gtttcgggcg agccggtgga tgcgctctcg attatcgtcc
     5041 accgcgacca tgcttacgag cggggacggg cgctggtctc aaagatgcgg gagctgattc
     5101 ccaggcagca gtttgaggtg gccattcaag cggccatcgg agcaaaaatc attgcccgcg
     5161 agacggtttc ggccctgcgc aagaacgtgc tggccaagtg ctacggaggc gacatttccc
     5221 gtaagcgaaa gctgctcgaa aagcagaaag aaggcaaaaa gcgcatgaag cgcatcggca
     5281 aggtggacat cccccaggag gcatttctgg ccgtgctgaa ggtgggagaa gaaagcagcc
     5341 gttaagggca cggcttcagg cttccggaac tgagtactgt cccacgtgat gcaaggtgag
     5401 agaaaagacc cattttgtgg gccttttctc cagccggagc gggtaccagg gttttcactc
     5461 tcctttaacc ctgcctgtgg aaagccgaag gcgtgttgct atagaggttt cgagggcctt
     5521 ttcaaggaga taattcggca tatgctggtt ctctattgaa aacaaagcag ttgcacagca
     5581 tctggcgtgc cgatcctgga atttacaaga ttttaaaaaa cttcaggcgg tgggagggca
     5641 attcttccac atttttttcc acagcaggac gggtttcgat tcagaaataa aaagaccccg
     5701 aagtaaccgg ggtctttctt ctggagagtg ccaaaagtat ctacttcttg ggtgcggtcg
     5761 tggtgggatg tgcagttgtg tggggcgccg gggccgacgg ggcgctggct gcgctgccag
     5821 cagccggagc agggacgcca gacttctggt tgtatgcctg gatgaccgca ttggtaatgt
     5881 ttgtggcttc gctggcccag aggacggggc tgctctgcgc gctgctgtca atcaccacag
     5941 aatagccatt ctgctgtgca tatgcctgaa gcacgttgta taccttctcg gccagttgct
     6001 ggtaggtttg ctgcaggtcc tgctggaagt cgttctgagt gtcttcgccg aggcgctggt
     6061 attccttttc cttatcgtca atggtgcgca ggcggttctg gcgctcggtg tcgctcaggt
     6121 tattgccggc ggactggagc tgctttttca ggccgtcaat ctcatcgctc atctgcttaa
     6181 gctgctggcg tttgggctca tacttcttct gcagttctga gaagttgcgc tggccttcgt
     6241 tcgtctgcat gacagcgggc tggaacgcga tgatggcaat ttttgtcccg cccgcaggtg
     6301 cgacagcggg ggccgcgccg ggcgcggggt cagtggcgcc tgcctgggcc atggctgaga
     6361 ggccaagtcc agacgcaagc atgcaaacga tagctaacga acgcttcatg gaaatcagaa
     6421 actccttcgg ttctcggatg tggtcgcccg catttctgtg cggaagcatg gttcttcgct
     6481 ccctgcattt ccggtctgag gcgcttcgtg caggatgccc tcaaatcccc tgaccgggga
     6541 gaaggcacag acagaaacac ggacagcgga cattttcagg aactcaagcg aattatatag
     6601 aaatttgggt atcggcggtc aaattggatt acgtatgaga tgaaaatata gctttgcaac
     6661 ggcctttggc ctttatcgct catagacttc gcggcggtgg ccgattctta aaacaaggat
     6721 tgtaagcacc tcatcctgca atgagcagat gaggcggtag tcgccgaccc ggtatttcca
     6781 tagttcgcca aagcgagggc catgaagagc ttcgccgatg ctgcgcgggt cttcgagaat
     6841 ggcgaggcgc tggaacaaaa acttcaggat acgacgcgcg atctgaggat caagtttgtc
     6901 gagttctttt tctgctgaag gatcaaactt aatggtccag gccatatcgt ttcataatct
     6961 tttctactgg aaccagtttt gtcttgccag ctttgatagc ttcgagccgt ttttcggctt
     7021 cgtagatgtc ttcaagctcg tccagatggt gcagaatcgc ctcgcgcaca tagtaggact
     7081 tggtgcggcc ggtgcttctt gcgagtttgt cgaggcgggc ttctatctct tcaggtaacc
     7141 ggatggccag cataaatctc tccctgctat acaagtatag caggctgacg gggcgctccg
     7201 gataagcgcc gcttccgcgg gagatgtttc gtcaatccgc aaaaggcggg gttttccgag
     7261 ttttcctcat tccgatatgc cgtaaactca tagcgatgtc tcctgcgcgg aagctccaga
     7321 tggaacccgg tgacccggag caggaaaatg tctctgccgc tacccgaaaa atgcgcatgc
     7381 tgcgctcgct tgtctgcggc aacaaatacg agaaagctgc gcgcgcccag ggcgccacac
     7441 tcgtcgcggg agtagacgag gtgggccgcg gatcgctttt tggatgcgtt gtggcggcgg
     7501 ccgtcattct gcccgaaaac acccgcatcc gcggactgcg cgactccaag cagctgcttc
     7561 gtgaagaccg cgagcgtctg gccaggatcg tcgagcagaa ggccattgcc attgcggtgg
     7621 aagaggtaga tgtggaaacg attgaccgca ttaacattta tcaggcgtcc agactggcca
     7681 tgatgggggc cgtcgccagg ctcgcgccgc agcccgacca tcttctgata gacgcgctcc
     7741 accttgacct gccctgcgcg cagaccagca tcatctatgg cgactcgctc tcgatttcca
     7801 ttgccgccgc atcggtgatc gccaaggtct accgcgacca gcgcatgcgc gaactgcacg
     7861 aaatctatcc gcaatacggg ctggcgtcac acaagggata cagcacgccg gagcatctgg
     7921 ccgcgcttga aaagtacggc ccaacccctc tgcaccgcaa gagctttcgc cccgtatcgc
     7981 agacaacgct gccgtgggac atgccgctgg aagacacgat tgcggaagtt ccggccatcc
     8041 aggtggaata acgcatctgg ttttgtcatt gccacgggcc agcttgcaca atgaactttg
     8101 cacccgtttt ttgcagggcg cctttcgcaa ggactgcccg atgtacagcg cacgctgaac
     8161 tgggtgcatc ccgcctcgtg ttttcctgtc cgcgtgcgca ttgaaaatgc ccccaccgac
     8221 attcttcgcc tgagcggatc cgccgtagtg gtcgttcgcg gggaatggac gaaaatgcag
     8281 gtcttttcat tcctgtgtga cggccggagg cagatcccca tcatacaatt acattggttt
     8341 tcaatcagga actttcgaaa tttgtgctct ctaacctgaa ggagtgattg catgcctcat
     8401 acaccggaac aggtcgaaac gctcaagcat gctcccgcgg cagatggagt cgcagatgtg
     8461 atctatgcgc gctggagtcc tcgcgcttat gcagacaagc aggtttcctc agaagatttg
     8521 aggaaaatct ttgacgcggc gcgatgggcc gcatcttcct acaacgagca gccctggcgg
     8581 tttttcgtgg gccggcgcgg agatgcgacc taccagaaga ttttcgacac gcttgtggaa
     8641 ttcaaccagc aatgggccaa gagcgcgcct gtgctgattc tttcagttgc cagcaaaaaa
     8701 ttcgcgcaca acggcagtcc gaactatcat gcgctgcacg acacgggagc ggcgacggcc
     8761 acgctttctc tggcggccac ttcgcttgga ctgcacacgc attccatggc cggatttgac
     8821 cacgataagg cgcgcaaggc attcaacgtt ccggaagatt acgacatcgg agcggtgacg
     8881 gcgttggggt acctgggtga tccggagggg ctgcccgagc agatgaagaa aaccgagctg
     8941 tcaccgcgcc agagaaaaga tctgggccag ttcgtatttt ctgagtggga aagacccgcg
     9001 gtgctttaat atcggaagca atgtctgctt cgatcacggc acaggagcgg cgcgagcggg
     9061 gacgcctgca ccgcaagcag gtgcggcggc aggaccactg tctctggtat ccgaaagaga
     9121 gacggcacga cccggttgcg cttctggaag agtccatgcg tgggcgcgtg ccgtcactga
     9181 ttgcgctgaa atatgagcga atggcggcct cgcccttcgg cttcttccgg ggagctgttc
     9241 cggtgatggc agcagacctt tcgctgctgc cgcacaccag catcatcaac cagatctgcg
     9301 gcgatgcgca tgtgcgcaat ctgggcgcct atgccgcgcc cgatggccgt ctggtctttg
     9361 acatcaatga tttcgacgag accattcgcg ggccctttga gtgggacctg aagcggcttg
     9421 ccaccagcct tgttcttgcc ggacgtgagg taggagacaa gcgcgccgtg tgcgaagagg
     9481 ccgtacaccg gcttgttttt cagtaccgca agtatgcaga cctgtttgcg cgcatgcctg
     9541 tgctcgatat ggtgcgcttc caggtgcacc gcctgcagcg catcagtcct gtttctgccg
     9601 cgctgctgaa ggctgaacgc gctactccgg tgcatgcgct ggagcaactc actgttccag
     9661 cgaagggcag ggccggagaa gagggcaagc gcgtctttaa agaagagaaa ccggtgctca
     9721 cacgtctctc cgcagcgcag gcgcgtgctg ttcttgcgtc attacaggaa tacaagcagg
     9781 ccctgctgcc cgaacgccgc cactttttcg cgcagtaccg cccggtggac gtgggcttca
     9841 aggtcgtcgg cacagggtca gtcgggctgc gcgattatct tgtatacatg gaaggcaatg
     9901 gcgggggcga cccgctgttc cttcaaatta aggaagaacc ggcgtcggcc tatgcaaagt
     9961 atcttgagta ccacgggccg catcatcagg gacagcgcgt ggccgaagga cagcgcgcga
    10021 tgcagttcca gtctgaccca tttctcggct ggacaacgat cggccatcgc cactatctcg
    10081 tccgtcagtt gaatgaccac aaggcgagca ttgagataga agacctgaag ggacaaggcc
    10141 tgcttgaata cgccgagatg tgcggagaac tgttggcccg cggccactcc cgttccggcg
    10201 acccatgcct gatcgccgga tatctgggca acggcaagcg ttttgccgaa gcaattttga
    10261 aattcgctga agcctacgcc gaccagaccg aaagagactg ggaagcactg cgacgatcaa
    10321 aacatatggg cagaaacagg aacgcctgaa gtttctgaag ctttcgtctg ctcaaaactt
    10381 gcttcttttt catcgagggc cagccacggt caatcccagt gcttttacat agccgccgtt
    10441 cacgcgcgta tgagcaaact ggattttctt cagcagatca tcgaaggctt cctgagcttc
    10501 ttctactgag ggacgggccg cgatacgctt ttcggcattt cctgttccgg tgggaagttt
    10561 agcgaaggag atgatgtcgt cccagtgcgc cgggcggtcg aaggtgacga cggacgaggt
    10621 tgcgtccatc tttttgtagg gccagaagca ccatcccaca cgattcgctt cgagcgtttt
    10681 gacgaaggcg gcgatccatt cgtccttgtt ctcgccggac tcgcccagcc agatgggcac
    10741 gtggtattta tcgcggaaat ccagatattc ctggatgacg ctcttgtcgg ttgccgtcca
    10801 gtatttgtga aaggtgtaca tcacgttgga gtcgaatggc gggccgaaga ccgtgaagtt
    10861 cgagtcccac tgggcaccgc ccagtatcac aacgtggttc ttgtccacct ggcggaccgc
    10921 cgcgaccagt ttgcggtaga ccggttccag atcagggttg tattgctgca atcgcggata
    10981 gtgcggaacc ggctcattga gcaggtcgta acccaggaca accggttcgt ttctgtagtg
    11041 cgccgcaatt ctgcgccaga tagcggccgt gtgggcctgc gccgcagcgc tgcggtagag
    11101 ccagggatag ccccaactgt cgtcaatgtt ggagccggtc tgtccacctg gcgcacaatg
    11161 cagatcaagg atgagataga tgccgtccct gcgcgcccac gtcaccaggc ggtcaagcag
    11221 gcgaaagcct tcggcattct cgctgtcaaa aaacttccag tgcaagggca cgcggacaga
    11281 attgaagccg ctctccctga tccgatctat gtcgctttgc gtgatgtagg tctcgcgcca
    11341 ttgcttccag aaggcgtcgg ccttctccgg gccaatcagc tcgtagctca gttcctcaat
    11401 ttcgcgcgga gactgcgggc cgccgtcaaa gtggaacatg tagccttcag gctcaaacca
    11461 gttgcccaga ttgatgccgc gcagcatcag aggcctaccc tggtcgtcta ccagttcagt
    11521 acctttggtg tggacaaacc cacgctgggc atacagggcc gggccaggag aggcacacag
    11581 caacgacagg cagaaaacgg cagttttgaa aaatttcatg atggcgaaag gatataggaa
    11641 agataaacgt ttgtcttagg cgcgccagtt ccattcaggg ccccctgcac caggatggga
    11701 agatcatctc catttcattt cagcgcaggc ttggattttt atttaaattc cgtcaacttt
    11761 atttacaggt agttcgtctc ctcttaagga ctacttaatg tttcattttc tgccctgtcc
    11821 agaaagcagc gggcaggctt ttcccgagga gaaatttcag accagccacc tgtgaggatg
    11881 gccgtaccac aactttgtct ttccggcgat gtttaaggag agagcagatt caggcaggtc
    11941 cattgtgaaa tttcgtctta tttttgttct attttgcgcg gttgagctgt ttgttcttct
    12001 tattgcgttc gaacaaccgg cccatgcata cgtagacccc ggttcaggac tgttgatttt
    12061 tcaggtggcc ggctccatgt tgaccggggc atgttttatg cttcgcagga aactgcgcag
    12121 gctgtttggg cattgggggc aacagaaccc gcagcaggaa aaccaggtca actcagacga
    12181 ggcgcagact tgtggtgcaa actcctctcg ctgatgtttc ccggattgca accttccgcg
    12241 atccggcagg atcgctgcgc gttgaaaacg gccgtgttct gcgcacgctg cgtcacgaat
    12301 acgccgatac cgtcctgaag ttcattcaat ccaaaaccgg cgaagagtgg gtaaactccg
    12361 gaaggctcgt ccggaccacg gtcctgcctg aacaggaagg cgcaggagag cacctgctgg
    12421 agcacccgca cattttcttt ccttcttacc cctgggaatg gacaccggga caatgggctg
    12481 ctgccgcgga actgacgctt gatctctgtg aagcccttct cgaccacagg ctgattttga
    12541 aagacgctac tccgctcaat gtactctttg acgggccgca gccggtcttt gtggatgtct
    12601 cttcggtcga gcagcgcgat gtttccagtc cgatatgggc cgcctgggcg cagtttgtac
    12661 gcacatttct gcttccgctt gccgcttata aatatctggg atggccgctc gccgccagtc
    12721 tgcaaaaacg cgacggttac gagcctgccg atctgtatcc gcatctctcg ctgtggcagc
    12781 ggtggagcag cccgctgtgc tcactggtga cgttgcccta tctgctggag aagcgcaaag
    12841 gcgctgccag ccggatgccg aagcaatcgc cagagatagc aacggctgtt ttgcgccgta
    12901 atctgcgctt gttacggaag atggtgcgtt ctctctcgcc gggaaaatat ggttcgcgat
    12961 ggagcgccta tccggaaaac gctacccact acagcgcaga ggaccattcc gtcaaacaaa
    13021 aatttgtatg caactcgctt ttgaagatgc ggccgtccgc tgtgcttgat ctgggtgcaa
    13081 acacgggcac atattcgcgg atcgctgccg gcctgggagc gcgtgtggtt gcgctcgatt
    13141 ccgatgcaca ggcattggag cggaactggc gcgacgcgca agcacagaaa ctgtctattc
    13201 agccggtcgt ggcggacgta gcgcgtccca ccccggcggt tggatggcgc aacgcggagt
    13261 cactctcgct gctcgaccgt gcccgcggcc gctttgactg cgtgatggcg ctggggttga
    13321 tgcaccatct cctgctctct gaccaggtgc cgctcaggga gatttcgcgc ctgatgcgcg
    13381 accttactca gcgctggctg attgttgaat gggtcccggc agcggacccg aaatttcagg
    13441 agctgttgcg aggcagagat gccctctatg cccatctgac agaagaaact tttctcgatg
    13501 catttggcca tgattttgcg cgcgtgcttc gggagccgct gaagaacggc cgcgctcttt
    13561 ttctgctgga aagacgatag tgaggtgggt ctgaagctcg ttgcattgag gaagctatca
    13621 gcgagtttga aaagctgtgg ccgcaaagcc cgattatgca gaagccatgc gcaatcaagc
    13681 acgctttctg cgtgcggcca gagaaaattc gcagaccgta cccgaaaata ccgccaacgc
    13741 agtcacaagg ctggtccaag gatgtgtcga agatagtacc ttccccatcc taacgcgcca
    13801 accgcggacg gattaggatg ggggtacccg aaagcctgca aaataaaagc attgcaggct
    13861 ttgctctaat caatctgctt gaagatttcc gaaaacgcaa acggctgttg cgaaaccccg
    13921 ctgcatgtgg gttctgcgga tgttgagcca tctacacagg cgctgtcgcg tgttgcagac
    13981 cacatcgaca ggaagccaac gccgttgctc ctggcccagt tcagcagaag ctgcgcgtcg
    14041 ttcaggctga aggtttctcc ctgcgtatca ttcacgccga tcattggagt cacacctacc
    14101 atcgccagag cctgcgaaga cgataactcg ggatatagcg actgaagctg ttttgctgtt
    14161 gctgttccgg cgttgatggc gtcctggccc atctgcgaat cgggctgccc gtaatccatg
    14221 gccattgcgt ttaccgtctt gagcttcacg cctttgctaa tggcgtcctg aatcacgttt
    14281 agtccgtcct gtgtaaggcc cgtgggcagc accggcaggg taaaggagac ctccaaccca
    14341 ggatttgctg cctgtaaagc agcgatggcc tcgtcacgca gaccaatcga agttgtgtcg
    14401 gcttcagctg caccttcgat gtcgaagtcg atgcgcttga gctgatacat atcgatcaca
    14461 gcctggtact gcgcttgcag cgcggcttcc gtcgagcagg tttgcgccag ctctgagccc
    14521 gcttctccgc caaaggagac aataacatcg cctccccctg cacgcaggtt ttgtacatcg
    14581 cttagcatcc agttctgcga aagtggaacc accccacccc atgatgccga gcagccgcca
    14641 ccatcgacaa taaacgccat cgtaaagtag gggcccgcgc tgggagctga ttgcgtcagg
    14701 ttaaatgttg gatagaccgt aacgtccaca taaggtgcga agaagtgtga tggaaatgcg
    14761 ccatcggccc caccgccact gtttccactg ccgcccgatg ttgttacggt aatggcgctg
    14821 ctctgtgaag agatgacacc cgcgctgtca accgcagcta ccgtaaattg ataggttgta
    14881 gatgcggtga gtcccgtgat ttttgtgctg gtggttgtag atgaagtcag gagtttgccg
    14941 ttttcgtaaa cgttatagct agtgattggg tttgtgccct tggcggcagc cgtccatgaa
    15001 agtgttacgc tgctgctggt tacatctgca gcagtcaggc cagtgggcgc tcctggcgga
    15061 acagtgccgc tgcccgtggt gcaggtcgtc gagctgaccg cctcaccatt caccaggcaa
    15121 ttggctggat tttgcggtgc attgcctgca taggcgctgc caatgaatcc aaagttcact
    15181 gaacctcctg caggaatact tatgtcccag ctatcaccga tgatggtgta agtgttttcg
    15241 gactgcgctg cgacctgtgc attccagatg ctgctgatgg cgtatggaag ggtgaattgc
    15301 agcttccaat tttggatggg tgtgctgcca gtgttggtga tggtcacgct tgtctggaaa
    15361 ccggaccccc aggctgacgt aacggtgtaa gcgacttgcg gggcctgcgc ctgcagggtt
    15421 aacggacacg cccaacagat gagagcgagc acagcgcagg caacaatggc catgtatttt
    15481 gaataaaggc gcaaaccaat gcgcatgggt tcctccagta aaaattgttt ctacagctat
    15541 cgaaaagcta agcaatgctt ctccgggttc ggattttaag gcagaaatat ggaagggatg
    15601 cagtggaagg tactgatatt ggggagtctt ctgcgcgctc ccagtctaaa gaatagaaat
    15661 aagtatcctg tatagacaac tggactgtca atgaaaattt tttgttcgat ggatatcgaa
    15721 tatctaaaaa aattagatgt aaatatgcaa tttggaagca taaagcgaat atctggattc
    15781 agaatccgct ggaccagaaa tcgctatggg gctgatgaca gaactactaa tcgtggctgg
    15841 taattgactg tgccattttc ctgagacgca tattctaccc aggcagaact accagttgga
    15901 gtatctggag cgatctggat cgaaagttgc tgtcccgctt ttgccgcggc tgtgacatca
    15961 aagtatgtca ggataccggc accgaaagaa atgccagtag acaatcgccc ggaaaccttc
    16021 ggttgattgt tccaggtgat ctcatcagca atccaggaat tatcagtggc gtagttggcg
    16081 aagttgtaaa tgttgttttc tccggcgctg gttggagtca gatatagagt ggcctgtaac
    16141 acagtgccgg tgatctgtga gaggtcaaac ttgagccagg aattcctgtt atagcccgtc
    16201 gcatcttgct ttacgaccag ggttgaagat ccgccgaaat tggtatttgc atagctcccg
    16261 tcacggacat aggcatccgc tatatcagag agcgtggttg aagttacagt tgtactgtat
    16321 ggataaatgc gaacatagtc cacatccagg tatgcattcg tcgaggaccc caaggggccg
    16381 taaccgcccg ccggaacata cgaggcattt ggaagctctg tgtccagaat aatgtactcg
    16441 gcaatatctg aaatggctgc atcgctcgcg ttcagagacc aggtctgttg gccgtcaacg
    16501 tagaaggtga ggttaccagg agtccatgca aggccaaacc gatgaaaatt gccgtcgttc
    16561 agattgctca acgtaccgac gtgtgaggct gattggctat aggctccata accgttccag
    16621 atcaccgcat ggtcgtaacc ggtcggagtg gcgttaaagt gggtgtgttc aaaaacatcc
    16681 atttccacac ccgactgttg tggattgcct atggtcgcgc caactgtcgg actttgtatc
    16741 cagaatgagc attgtatgcc ctgctgatac tgatagcgga cgttggcttc ccagtatcca
    16801 tatgcatgga gaaaggagcc attctgggtg gtaatagcgc cgcaatagtt tgtgggaaca
    16861 ccctgggcat tgttagcagt gtagatgctg atcctcgcat atccattcgc tacatggact
    16921 gcactggaat caataaaaca gtcgtcgcgt tgtgtccctt ctccacgata agaccacacc
    16981 gatgtatcga gggagctacc gttaaactct tcgtcaaaga tgggcgtagt cccgaaaccg
    17041 gcaggcactt gcgcgacggc aatgcctgcg cttccggcca atatgaccaa gcctagaaca
    17101 cttcgtttga ggaagcgttg cgccacaggt cgaaacatag aggatgctct ccttaaatca
    17161 tgaagaagct tctgtacgtc tttacgagat tgcgcgcaac gatctattgc aggggccctg
    17221 tttggcaagt gagatacggt agcctggtcc gttatttttg atttctcctg aaaaatgaat
    17281 atcttggccg ccacgtaaca aaagaagaag ggcgcagtaa cacggcgtct taccagaact
    17341 ggcttgccac tacggttgca gcacctgtat tgccgccgac tacctctact ttcaggacgg
    17401 cctcaaaatt gcggcttgcc catccaggtg gggcagattt ttccagttcg gcgagtttct
    17461 ctggtgaaac gatgaactcg cccgccgctt ccgtaccatt agagcttatc cccgccacca
    17521 cgatgactgg gccaccggtt gtggcactat caaagcgggc aactatggca tagtccgtcg
    17581 tttggccaaa atatggtttt gagaaatcca cgttccatcc gcgataggct ggatgctgcg
    17641 catcctctat atatgcgaag tccccttcct gcacaaagtg aaagcgaagg ggcctcagta
    17701 acagcaaggt ccatttattg ttgtttccgt ttaccaggac aaccggcctg tcgtggagat
    17761 cggcaagtgt attcgaataa gcttcgtgaa tacggaaggg tttctggtgt gcctgcagaa
    17821 aaccaacaat attggcaatc gcactgacgg ttgtgatgga aacccggaac tctgggcgca
    17881 aaatgtggtc cttgatactg atattaggca actcaggcgg ctccatgtcc tgatgagggc
    17941 gtcctgcgct gatcaataca ggtccggaga ttttcagaag cggggtccac actgccatga
    18001 caggatcagg tgtctgaagt tttttataag catagatgcc tccgatgcct gtcgctatca
    18061 gcaatgcaag tatgagatac accggccaga ccatgcgtct ttttccggca tatggaaccg
    18121 ctggctcagg cgatggccca ttggctggaa gacctgaatc caaggctggt ccttccccgg
    18181 gcaagactga tacggctcgc cggtggaacc tgggaatata ggttcctaaa ggaagctcaa
    18241 cctcaaccgg ataatctttc gaggtgtcgt ggtaaagctg ggcgagacgc tttctcacct
    18301 cgccggcact gaagcgcacg accggatcgg cattggtgtc atagcccgga tcgcgcccga
    18361 agacctcgac accaatggtt ctttccttta agcactcctg ttgtccttcc agtgtctttt
    18421 cgacgatata ccgaagcatg gcaggatagc gccgactact tcggaatggc gcgctggcaa
    18481 ggatttcctc aagcatgtta tagatgaggg cctggtcttc cgcactgctg ggtatccagg
    18541 cctcgcgact tagagagtca ttgcccaaag agcccgcttt catagaggaa atctgaaaag
    18601 agtctcccca aatgaaaagc cgcttgtcaa cacaggactt gttacgcccc gctttcgcgt
    18661 tgttacgtca cacgaaaata cttgattatc caacagataa ctccatcagt tcatgaaata
    18721 ccgtatacca ccttgccaat tcatgtgcgc aaaggagatg acataaagcc acagtaacaa
    18781 aacagtttct gaaggcaaat cagcaagaaa agttagcgca caaaaatggg ttggttcaag
    18841 gtcgaagctc aggcaaaaat tcaaaagcac caaagccatc cttatgactc acagtacagg
    18901 cacgcttctt agggggcaaa atgacggctg gacagtttcc atatttatca gacaaggtca
    18961 aaacactctc aagcaggcta tgccagggta tggtatttgc cattgctttt atcctggcag
    19021 ttgcgtgttg cggaaccgtg actttcgccc aaagcggcgc gggcgccatc cagggaacca
    19081 tcacggattc taccggtgcg gtaataccga atgcagcagt ccatgtggtc aaccaaggca
    19141 ctgggcagtc cttcgactcc actgcgaaca ctgccggatt ctattcagtt ccggcgctct
    19201 ttgcaggaaa ctacacagtc accttcacgt cgccgggaat ggcaaaatac gagaccagca
    19261 tcgccctgca agcggcccag accgcagtta tcagtccaat ccttaccccg ggggcggtgc
    19321 agcagcaggt tacagttacc gcgaatacca ttcagttggc gacgtatgac agcggcacca
    19381 tcagctcgca actggacaat aacagaatca accagcttcc aatgaatggc cggaatgtcc
    19441 tgacgcttac aggaatgaca acacccggcc tggaatcggg cggaacgcgc gccaatggca
    19501 acatgcaggc ggccatcgag tacgtacagg acggcgcgcc tttgtctgac cgcaacttcg
    19561 gcggcccaac catcatgcca gaccccgatt ccatccagga agtgaaagtc gaaactgtag
    19621 cctcgaacgc caggtttgct actccagcaa cagcgattgt tacaaccaag tcgggaacca
    19681 accaactcca cggaactttt tttgagacgg cccgcaataa tgccatcgga attgcaaagt
    19741 cgcgccagaa tgctccgaac tttgcagccc cgcatttggt acgcaatgag ttcggcgctt
    19801 cgatcggagg cccgattttt ctcccaaaac tttatgacgg gaaaaacaaa tcgttcttct
    19861 tcttcgctta tgaacggtac tccctgcggt cagaacagga cgaattggta acagtcccca
    19921 cgcaggccat gcgcaatggc gattttagtg gcctgataaa cagcgcaggc cagttgcagg
    19981 tgctttacga cagcaatacg acgaatcctg tgacatttca gcggcaacct tttgcgaaca
    20041 accaaattcc aatgggccgc ttaagtcccc ttgctaagac gctgtattcc atttcgccgc
    20101 tgcctacttc ggctgacaat ccgatggtca gcagtaactt ccgcgctccc gggatcaaca
    20161 attcaaccgt gccgaacatg accgcgcgat tcgatcacac atttggtagt cagaacagcg
    20221 cctatctgcg ctttacctcc acgcaatcaa ccacgctggg cctccgcaat tatccgacaa
    20281 accaggctga cacaattggc ggctctggtc tcccggcagg ggcaagcaac gaggggctta
    20341 ctaaagtctc cgtgtacagc gctggcctgg gttacatgca tgttttctca cccagcttct
    20401 tttccgaaac ggtactcagc aatgaatggg aaagcggcca ttatggagca ggtggaaacc
    20461 caagcctgaa ttatgaggca atgctgggcc ttccaaataa ttttggatca accggctttc
    20521 cccagattgg cagcacctcg ctgttcatga actatgctgg aacacagttt aactatgcgt
    20581 catcacagat tctgaccaac ctggatgaaa acctctcaaa aacaatcgga cggcaccagc
    20641 tctttttcgg ggggcgctac cgtcacgagc ggctgggcgc gctgcccgat cgcactccgg
    20701 atgcgcttga tttcaacgga atgggatcag ccaacgtaaa cccagcctcc ggaaagtcct
    20761 acactgcgct gcctaacacc ggtgatgcaa atgcggacct ctttcttggg gcggcatact
    20821 attacaccat ccgcctcaac ccgccgtatg agcactggcg caaccaggaa tttgacagct
    20881 atttccagga caacttccag gtaaacagca agctgacaat cagcggcggt cttcgctggg
    20941 aaatccaccc aacagcaaca gaaaagaaca atgtgttcaa tggatttaat tttaaggaca
    21001 aagccattgt tctcggccag ccggtttcca ggtttattgc aaaaggtttc accacgcagg
    21061 cgatcattac caatcttcag aatctcggcg tcacttttga gagtcctcaa caggcgggcc
    21121 tgccggaaca tatggtatac agcaataact tcgtgtttag cccccgtata ggatttgcat
    21181 acgcgccgtt tggaagcggg cgcggaactg taattcgctc agggtttgga aggtatgctt
    21241 atccaattcc gataagaaac ttctattccg tcggtaagct gaatgccccc tttgccacgg
    21301 cttacacgca gagctatacc gctgcaaacc agtctcctga tggccagccc aactatcttc
    21361 tgcgttctcc tcaaaccgta gttgctggcc agaacagctc gaatatagtc aactcaaacc
    21421 agacaaactc tattcttcct ggtggtcctg gcgaagtcgt gctgaatccg cactatcctc
    21481 ccaacttcgt cacgcagttc aatttcacag tggagcagcc gctgaagctg gattctgttt
    21541 tgcgaatgtc ctatatctgg gaccacggtt cgaacctgga ccagttgaac aaccacaaca
    21601 atgcgatgtc atcgtatgtc tggcaagtaa caacgggtac attaccgcct acaggtgctt
    21661 atgcaagcgt cgccctgaat ccctatgaca atgtgaccta tggacaactt actacggtag
    21721 accgtacagg gtggtcgaat gacaatgcgc tccagctgaa ttatcagcgc ctttataaaa
    21781 agggttatgc ttatcagatt tactatgtgt tttcgcgcgc aatgagggtg ggtggcaata
    21841 ctttccgcga cagcaatata tacccttatg cggattatgc gccgggagca gcgccgtcat
    21901 ccgattttga caagctaaac cgttttcaga actaccagat tgattctgcg actccgaagc
    21961 accgtattag ttggaacgga atcgtggacc tgccgtttgg gcacggaaag acattctttg
    22021 gcaatgcggg gcgacttgtg gatgagttga tcggcggcta tcagattgcc ttcgacggga
    22081 ccgtcttttc tcagtacttt cagccctcat cttcctattg gggcggagta aacccacagg
    22141 gcgcaggctc tatcggccct ctgcacgtct acaaaaagaa gtacaagatt acagattgca
    22201 gcagcggtgt ctgtcgccct ggatatctgt ggtttaacgg ctttattaat ccacttcaaa
    22261 tacacaatcc ctgcggcccc aatgcgtaca ctggcattcc gtcgaactac acgccatacc
    22321 agactcctat caatatggat cctggcacgg ttacctgcgt aaatggcgca gtcaagacaa
    22381 gcaattcaaa ctacctgacg aacaacgttc cagtgaagct ggccaatggt tcaactgtac
    22441 agactgctta taatccaggc cctgctggag tgaacccctt ttcgcacacg tttctctctg
    22501 ggcccttcaa ctggacggcg gacctctctc tcttcaaggt gttccccatt acagaaaaga
    22561 taaacctcag agtaaacgtg gacgcgttca atgcgtttaa tgttcaggga tacaccaacc
    22621 ccagtccttc aactggaata cagacctttc tgtcttcgta caacacaccg cgccaattgc
    22681 agttcacggc gcgtctgacc ttctgatctg attaccgacc ccttgcttca caaggggtca
    22741 tcactttgta aatttggagg aataacagat gccgtttatc agcaggcgtg cgctcatttc
    22801 acgcggtatg acagttgcag ccattggcgc actgtgccgg tcctcatcct gggcgcgcgt
    22861 aatcggggaa gatacgtcgc cagtacattt gagcgtgtca gtggactggg aaaatgtcat
    22921 ttcatctgtt tctcctgaaa tgcttggatt aagttacgaa tcatctcagc tctctgcgcc
    22981 agatttcttt tcagcggcca atactgagct aattgctttg tttaaaaagc tctctccaca
    23041 aggcgtcctg cggcttggcg gcaatctgag tgaatacact gtttgggtct ccagccgcac
    23101 agaagaaaag ccgcggatac cattccgggc tgtcggcgcc gattcaggag ctaaaaaagc
    23161 agccatgccc tcgccggtgt ctgtgcaagc aattcgcaat cttcgtgatt ttctggaaag
    23221 caccggatgg cgcgctgttt atggactgaa tcttgcacaa gccgatcatg cccgcgtgat
    23281 tgatgaggct gtttttgttg cagacacgct cagggcgcgc ctgatcgcat ttcagatcgg
    23341 caacgaaccc gatcactatg tcatgaacgg gcttcgtgcg ccaggttacg gtttccagga
    23401 ttattttgct gaatggagcc ggatatacga tgctgtccga aaagccgtac catcggcaca
    23461 gtttgccgga ccggacatcg cagaagatgt tgcatgggta gatgcttttg caaaagctgc
    23521 acctagttcg gtcgccttcc tctctggcca ccattacgcc gaaggcccac caaccgaccc
    23581 aacgatgact ctggagaggc tgctttctcc tgatccaagc ttcaatcaca gattagcagt
    23641 gacagaaaag atcgcacagg aagcccgtat tccctacatc atggcagaaa caaactcctg
    23701 ttataacgcc ggcaaggctg gcgtgagcga cgtattcgga tcggcattat gggcaatgga
    23761 ctatttgctg caactggcgc aagcaggaac acggggcgtc tattttcatg gaggagctaa
    23821 cagctggtac accccgattg cgggtggatc cggccaacca ttttacgcgc gccctgttta
    23881 ttacggattg ctcatgtgcc gagatttgtt gggatctgaa ttagtttctg taaaggtcaa
    23941 ggcagatgcg gcaaatgtat ctatatatgc tgtgcaaagg ccaaaacgtc ttgttgtcat
    24001 caataaaggc ccgggcaatt tcaccatcga attgaatcgt ggtgaatgtt cagaacaggt
    24061 actgcggctg cttgcgccat cgcccgaagc aaagaccgga ataaaagtgg atttgcaatc
    24121 gttccatgca acagaaaaac gcctctttgt tcccaaatat agcgctacta tttttgacat
    24181 tcagtcttca tagctgcaga gcgtggaaat ccagtttgct ccacttacct ccctgcgttg
    24241 agagcaggac cttcccttac ggatgctctc aacgcatctt tgtatggccc tggggacact
    24301 tcacgaaaca gaaatttatg tacggttagc cgctctttta ttttgagtgt catgtgtaat
    24361 cgcccgtgag gttgcagaac gtcctattcg tgctggggcc ctaactctat tcgatttgcg
    24421 agggtctgaa tccctctctc ttccgccaga attcctttat gcccctgcta catcctcaat
    24481 tcaagacaac tgcttcaggt tatctggaaa tttgcaggtt tcgttgtctt gcgggtgcgg
    24541 tagatttgcg gaccactaat tctgtttcca ggcgatagtc tgcgccaatg cgccgtttca
    24601 gcttaagcat cttgtctaaa gcatgaaatg ctgtaatgcc caggcgttca cgtggaacac
    24661 ttatggtcgt gagcggtggg cgggcgagga aggcgaagga tatgtcatca atgccgacaa
    24721 tagagatgtc ttcagggatc ttgacgcccg cttcttcaag agcattcatg gctcccatgg
    24781 cgataaggtc gcttccgcaa aagattacgg tgggtatctc gggagcggag agaatggcct
    24841 tgactgcaga agcaccggca tccagccggt aatcggtgct ggttaccggg aacgggcgaa
    24901 ttccccttcg tttcaagcca gacagcaatg cctgcttaat atttgctgcg gtgcgattgg
    24961 tttcggggcc ggcaatgaca gcagcacggt gatggccgag tgcggcaaca tgtttgattg
    25021 cctgcgatac tccatgctga taatcgatcg tgatattgct gacaagtttt ccgggagggc
    25081 acaggttgca gaagaccacg ccaatgcccg cctcgatcaa atcaggtgtc atgctgttgt
    25141 cgattgagga ggtcacgatg gcgacaccgc gaacgtcgct ttgtatgagc ttgttgatga
    25201 cagattgcgt gcgccgtgga ctgtattctg tattgcaaag cagcacatcg aatccctgat
    25261 cccatgcggc agcctggaag ccgcggatga tttccgggaa ataagggttg gcaatctccg
    25321 aaatgacaag accgaagaga tcgctttggc cggtcgataa tcgcttagcg tgaacatttt
    25381 tgtagtaatt gagttgactc actgcttcaa gaacgcgctt ctcggttgcg gcggctatgt
    25441 agtgagtttt attcagcaca cgcgacactg ttgcgggaga aactccagcc agcttcgcca
    25501 cttctttcat tgtgtgggcc atgttccctc ttatatcttg cccgatcagg cacctcagga
    25561 agaaggatct gcaacttcct gagagcctag aaccctccaa gtcactgaaa agttaccacg
    25621 attcggataa ctgcaggaat agcgccggac aagaaatatg gagcgccgca gaatttgctt
    25681 gacaatccag atcttcggat tgcacaatcc tgaaatcggt ttctgaaatc ggtttcctat
    25741 cggccaatat ggatacctct acgcagtgct gatggatgcc ttgccatgca tttattagat
    25801 agtctccagg cacgtttaga agctcgtttg agcaggttgt tttggaggat tgacgcatat
    25861 gatcgaaaaa agaaacaagt gttggtattg gctgctggta gcagtaagcc tgattccaac
    25921 gcggcttgca gcgcaagccg taggtagtat cggaggtacc gttactgacc cgaccggagc
    25981 tgtcattgcg caggcgagag taacggccac acgcgaggac acgaaagttt cgcaatctac
    26041 gttaacaaat gaatcgggca catttgtgat cccgaacctg acagttggta cgtataccgt
    26101 gcaggtgagc gcgggcgggt tcacgacaag gagcattaca gggattagcc tggatgtcag
    26161 ccagcagcgc gatatcgact tcaggcttac aaccgccgga acagttgaaa cggctgaggt
    26221 gactgcggcg ccgccgctga tcaatacgag cgacggatcg ctggcggggc ttgtctctga
    26281 aaaacaggtt gaatctcttc ctctcaacgg gcgcagcatc cagaacctgg tcatgttgca
    26341 gccaggcatg gctcaggact caggcagcat gggctggctg gctccgcaat ggattagcaa
    26401 cggcaaccgc ggagagacgg aggtagcaac gctggatggc tccgatgcta cggacacgga
    26461 aatgggcacg gtgcagttct ggaatttcaa tctggacgcc atcgccgagt tcaaagtgca
    26521 gcaggcaaat tattcggcag agtttggaca gggcggcggc actatcactc agatcgtaag
    26581 caagtccggc acgaaccagt ttcatggcag cgcgtttgaa tttatccgca acagcgtatt
    26641 tgatgcggcc aattatttct catcttcggg tgtttcgcct tttcaaagaa atgagttcgg
    26701 ggcaacgatc ggtggcccca tcctccggaa caggaccttc ttctttggcg agtatgccgg
    26761 ttttcgccag agatacggca cgacaacgat tctcgaggtc cctaccgccg atcaaagaaa
    26821 tggtcttgtc actatcaatg gctttgagta caaggtgcca ctgaatccag tcgcccagca
    26881 aattttgaac aaatacccga tgcccaacca gcccgcaggc atctatggcg aaaataccta
    26941 taacacgctc ttcaagcagc ccactgatga cgaccaattt tcagtacgca tcgaccataa
    27001 gatttccgac aaggacagca tctttggccg ggcatcctat atcaacaaca gccagaaagc
    27061 cgttgatcct acagcggctg tcgaagaccc agctttttcc gcggaaaata tcaataatcc
    27121 gaggaattat gccgtcagcg aaacgcacat cttttcgcca aagctaatca ataactttac
    27181 attttctctt aaccggcaga ttgaaggcag cacgcctctc tcgcagcagt acacgcagac
    27241 gacgacagga gacgggacgc tttcaaactg gggccctgat acttttatca ccaagtatgt
    27301 agaaacctac tacataactt ctgacaaagt agattggaac tggggccggc agttcttcac
    27361 aattgggggt gactaccgct atggccagga caatggtttt ggtgtgacca gcgcaggtcc
    27421 gaatgggcaa tacacgttca ccgccggcac accgctcacc gtcgatattc cttcaacaaa
    27481 cggcggccca acgattcctg cgggaacagg cagtcccagc ggacttatca gcatcatggc
    27541 gggtgacgca caaagctata agcgctcgac aactatacca ggcttcggtc cagcgggcgg
    27601 cggcggcgta cattggggat tgcgcagatg gcatatcgct ccctttgtgc aggatgacat
    27661 caagttcaca cccaagctta cggtgaacct cgggctgcgg tatgaataca attccgtgcc
    27721 ttacgaaatt gagaatcgcc taggggggat tgccgataca ggctctctct atggccattt
    27781 tgttctgaac ccgcaacctc tttatcagcc tgatcgcctt agctttgctc ctcgctttgg
    27841 cattgcctac cgagcgaaag ataaaacagt gctgcgcgga ggcgttgcca tcttcacaaa
    27901 tgtgattccg atggtttatc ccgatcaggc ggcggtgaat tttccgctgg ccagctatag
    27961 ttcactctct aatccgccgt attctttgac tccactgccg gtatctctac caactctaac
    28021 cagtacgtca ggcacggtga tgccgccgaa cggcgggcca aagaaaatcc cacccaatac
    28081 tcctgtgaat cttgccccca ttgcggccga gactggtccg atcattggtg actggccttc
    28141 agacaagatg aagaatggat acacgttaac cggcaacttt accatcgagc agcagatgcc
    28201 gggcgacatt gctctgcaga tgtctttcat cacgaacaat gggatcagcc tctaccagag
    28261 cagttacccc aatgccttta ctggtgcgca gacagcgaac accccgttca cgaatgtgac
    28321 cccgggactg ggcgaagtgg aagtttttta caacgacgcc atctctcatt acaacgccat
    28381 gcagctgcag atgcggaaaa tatcgcctct gcatgggctg caataccaaa tcaactacac
    28441 ttggggaaag aacctgaccg atgcggacgc tgtttggagc gctccgggca caagcggcgg
    28501 cgtcacgcag aacgacccta cgtgcattcg ttgtgaatac gctcgcgcaa gttatgacct
    28561 cagacagcgc ttcgttgcca acttttccta caacattccg ggttcgtggg gactcgtccc
    28621 ttcgccaatc agcaagggat ggcaggtcct tggcatctac tctgctcaga gtggcttccc
    28681 attcaccatc gtaggcccgt acggtacgct gcagtatggc ttcgacactt tgaatggtgt
    28741 tggagcgcgg cctttcttta tcaaatgggc gcagcgcgat ccccaacatc gcgcgcaatt
    28801 cttcacgagc gatgcgatca acaacaccag cgcctacttc aatgagccaa ctgtaaccag
    28861 caatatttcg ggtgttggcg cggtgcagac atcgcccggc aacctgggac gcaataccta
    28921 cacgggaccc ggctggtgga atatggattt ttcagtgctg aaagatacac gccttacgga
    28981 agttctcaac atgcagtttc gtgccgagtt tttcaatatc ttcaaccacc caaccttcgg
    29041 cacgccagga ggtaccatcg gaagctcgaa cttcggtctt tcgacttcca cgcaatcctc
    29101 tgaacgtgaa ttacagtttg gcctcaggtt tatgttttag cagcgatgaa ggctgatcca
    29161 ggcacaggag gggttctgga taaataagca cttgcaatgg gcactgtaga agcttgtaat
    29221 ggcatcgccc tgacaatctt cgcaggtcct gaaaaatcat agagggagaa atcgaaacca
    29281 tgaataaggc gctcttttca cggcgcgaat tcttgcaaac gactgctggc gccgcggggg
    29341 cttccctgat ctccggagcc aattttctca atgccgagcc aattccttcg gcatcacagc
    29401 cggttgcgcc cagcgatcag gttcgttttg gaatcgtagg tgttgggatg gaaggttcgg
    29461 ggctgctctc aacggccatt cagcttcgcg gagtagagtg cgtagcggcg gccgatcttt
    29521 atgacggtcg ccaccaactg gcaaaagaaa ttgttggcaa accgatccgg acaactcgcc
    29581 ggtatcagga gttgcttgat gcgaaagaca tcgatgccat catcgtggcc gttcccgatc
    29641 attggcacaa acaggtggtg gtggacgctc ttgcagcagg caaggacgtg tactgtgaaa
    29701 agcccatgtc ccacaatccg gccgatgggc tggcgatggt tgccgcggcg aagaagacag
    29761 acaggatcgt tcagatcggc gcgcaacgta ccagctccgt tctttgcgca aaggcaaaag
    29821 aactttatga gaagggggcg atcggtgagt tgaatctggt tgaagcgacc tatggacgca
    29881 atgatcctaa cggggcgtgg gagtatcccc ctcctcctga tttgtcgccc gaaaacctag
    29941 actgggacac atggctggga acagcgccga agattccctt cgatcctaat cgttttgcgc
    30001 gctggcgttg ctggaaggaa tacggaacgg gtgttgccgg ggacctgctc gtccatctga
    30061 tcagtggcat gcaatttgtc ctgggcatca atgagtaccc gaagcgcgtg tcggcatttg
    30121 gcggcattta tcactggaag gatggacgca atatgccgga cgtgcatcct gtcctctttg
    30181 agtacggcaa cggtaacagc aacattcccg tctacatgcg gctgtctcta gcatgcgaat
    30241 ccactgaggt cacgcgtttt caggggccga aaggtgctat cgagctccgc gaattcagcg
    30301 tcacgtatat cccgcaggcg ggagtcgatc tttcgcccag ctactacacc gcgtcgtttc
    30361 cggcagctat gcggaacgcc tacgtcaagc agtggcatga ggaacacgat cctgttcctg
    30421 ggcaggaacc cgcgccggag tcgattacgt tcaatggaaa cgactatgac gatctcaagc
    30481 cgcatctctg gaagtttttt gaagcggtcc ggtcgcggaa accggttgtg caggatgcag
    30541 tttttggaca taacgcggcc ctggcctgtc atcttgcgaa tgagtcctat tttcgcaaga
    30601 gcccggtgta ttgggatccg gcttcggaga cgatcaagtc actcgaataa gtttcggagc
    30661 ataaacagga gaacctcgtc tccggggccc tccagatcct gcggattagc aaaaagcaaa
    30721 ggagttctga tttcgtgaaa ttgcagaggg taggcttcat cacaattacc gccattgcat
    30781 tgatcttttc ccccaccttc attcattcgc aggcaacagg ttcaagcgcc gcttctgccg
    30841 cacctttcct ggggcgatgg gacctgacat taaaggcccc agataaggag tatccctcgt
    30901 ggctagagat acgccaggag gacggacggc tgaaagcgcg gatgacgggc cgttggggga
    30961 atgcgcggcc tctccccacg gtcgaagtgt cgaatggcga gcttacattt gtttcaccca
    31021 aagaagaaga gggcagcaag actgatcttg tatttcaagg caagctgacc ggcaaaacgc
    31081 tcaccggtac agtcaacggt ccagacggaa agacctggca atgggttggc gtaagggcgc
    31141 ccgcgcttga gagaacagac acgccgaaat ggggcaagcc gatccaacta ttcaatggaa
    31201 aagacctgac cggctggaag atgagcgcgc caggacctcc tgtgtggacg gtccagaacg
    31261 gaaaccttgt cagtccggga aacggtcctg agcttatcag tgacgcaaag ttccaggact
    31321 tcaagctaca tcttgaattc aattgtggca aggattcgaa cagtggagtg tatctccgtg
    31381 gccgctatga agtgcagatt gagaccgagt cggaggcgga gccgccaagt catcacaccg
    31441 gaggcgtcta cggttttctt gctcccaatc cggagcagcc gcgcacgccg gatgtctggc
    31501 agacgttcga tatcactctg attggccgca aagtcactgt tgtcttgaat ggcaagacag
    31561 ttatcagtaa ccaggagatc cctggcatta ccggtggcgc gatcgatagt cacgaagcgc
    31621 agccggggcc gatctacttg cagggcagtg aaaagggtca tgtcgccttt cgcaatatct
    31681 tgattacgcc ggcgcaatga aatgtctgga gagcagcgat gctctacagg cccttcagag
    31741 cagatccttc taagatgagc aatgttgagg aaatgaatga cccggttttt gcaggacata
    31801 ctccgccaac ccgatgagct gctgcgagtg atcgaccatc tcaataaggc ggagcaaaaa
    31861 ttgctggaat ccgctgctgg agcgatacgt ggcgctcgcc acgtctacat tactgggatc
    31921 ggtgctagct ggaacgcagc gttgggtgca ggatacatct tccatgctgg aggttggccg
    31981 gtgtatatgc tcgacgcatc agaattgctg ctattcgctg agattcctcc agatgcggtg
    32041 atcctggttt tgtcgcgtag cggcagaagc atagagattg tgaagttgct tacgaaggcc
    32101 cgcgcagctg gcgctgccgt gatcggcgta accaactttg aagatggccc attggcgcaa
    32161 gaagctgata tccctgtcat cattccagtc aaagcagatc atggcatttc cgtgaacact
    32221 tattcggcgc ttgctgctgc ggcagcggct atagccaatg ccacggtgag ttcatttggc
    32281 gaagagctca tagccgcgct ctccagctcc gttgccgaaa cggcgaagag aattcctgaa
    32341 tggcggcagc aactggagaa aacttcatgg ctcctaccgg gtgcgccgta ctactttctg
    32401 gcgcgggggg ccagtctggc tagtgcgtat gaagcaggtc ttctttggga agaaggagcg
    32461 aagttgccgg caaccgcgat ggggaccggc agctttcggc atggtccaca ggaagtcatg
    32521 atgaaggatg ttcgcatcgc gatatggatc gacggagaac agatgcgcga acaggacttg
    32581 gcggtagcga atgatctgcg agacctgggc gcttccgtca tgctgaccgg atacgatctt
    32641 cctgaagatg ccggagatct ggtttttcag atggctccat cgtctcggca ttggcaattc
    32701 ctgacggaca ttattccggc acagctcgca gccgagcgcc tggcgcaggt cgccggtgtc
    32761 gattgtgatt cattccgctt tgcttcctac atcgttgaag acgatttcgg actactgccg
    32821 aatggagcta catccctggc gaagacggga tcaaatggaa atgaaacata gttgaattag
    32881 ccatgaatgc aattggcgca gtagatattg gtggaacgaa agtagccgtt ggcattgtga
    32941 atgaagacgg acgtctcata tcgcgtatgg aagctccttc cagatcggtc gaagtctata
    33001 gcgatggagc gaggctcatc acagacatgc tacgtcagtc tacgcaagca acagggcagg
    33061 ccatagtggg catcggtatc ggtgcaaccg gcccggtcaa cccgtttacc ggacgattcg
    33121 gcgatctgga ttttctgccg caatggagcg gcgagagtct agttgacgat ctggagagag
    33181 atttccgtgt tccggttgcg cttgaaaatg atgcggacgc tgctgccttg gccgaagccg
    33241 gctggggcgc gggaagaagc aagtcgcggc ttatttacgt cacagtcggc actgggatcg
    33301 gcggcgggat catcctggat ggacggctct atcgcggagt cgatggggcg catcccgaga
    33361 tcggtcatca ggtgatcgac acgggtggtc ctctttgctc ttgcggattg aatggatgct
    33421 gggaagctct ggcagccggt ccggctatgg tgcgatggat ggagagccag ccagagtact
    33481 tcagcagtga agaactcaca gcgaaacgaa tctgcgagct tgcatatgta ggcaatgcac
    33541 tggcgcttcg cgctattgaa agagagatct tctatctagg tatcggtctc gcgaatctgg
    33601 tgaatgtatt cactccggat gcgttgattc taagcggcag cgtgatgaaa agcgctgacc
    33661 tctttctgaa tggcattcgt aaggtcatgc gtcaaggatg ccgattcgtt cctttggaga
    33721 aagtgaaact gaccttggct tctctaggcg aagacgcgaa ccttatcgga gcggcccgtg
    33781 cctggcatga acgcttcgcg cacggcgaga agcatgtctg tcaatgaaag atattcaatt
    33841 ggcgatcgct cttatggccg gcgagagaat gtaatgggag tcaagtgtat atggctagaa
    33901 atcggtacat ggtctatctg gtcttgctta cattcttcgt catatcccta cttacgaaca
    33961 tcctcggacc gatcgtgccg gatattatca gcaactttca tgtaagtctc actgccgcgg
    34021 gatttctggt cttctctttc ttcattgcat acggggtcat gtctatccca gcgggatttc
    34081 tggtagagcg cttcagggaa aaaccagtga tgatatcagc ctttgtgtcc gctgccgtcg
    34141 gctccatgag tttcgcgttt tttccggaat ataaagtggc agttgtttca ttgttcgtca
    34201 ttggcgctgg aatggccagc ttgcaggttg ctatcaatcc tttattgcgt gtctccggcg
    34261 gtgaggaaca cttcgcattc aacgaagtgc tggcccagct gctctttggc actgcgtcat
    34321 ttatcagccc tcatatctat tcttacctgg tcttgaacct gcatcggaat ccgcaaggtt
    34381 ccaacttgct ggtccgtggg ttaaacaggg tcactcccat tgcgctgccg tgggtctctc
    34441 tttactggat tttcagtgcc attgccgttg cgatggttct ggtgctgtat ctagcgagat
    34501 atccgcgcgt tcagcacact gacgaagaac gccctggcac atggcagatg tatggatgct
    34561 tgatacgccg tcgccttgtg tggctgtact tcttcgcaat ttttgcttat gtaggatgcg
    34621 aacaaggttc cgctaactgg atttcccagt ttctcaccca gtatcatggc ctcgatcctc
    34681 atacaaccgg cgcacgtgcg gtctcatggt tctgggggct aatgacggca ggctgcctgg
    34741 ctggagtcct gctgctgaag atcttcgata gcaggcgcgt tttgatcgga gcctcagcgg
    34801 gagctctctg ctgtctgtcg ctagcacttt tcggctccgg gagaatctct gttgttgctt
    34861 ttccgttggt tggtttattc gcttccgtca tgtggcccat cctgatctcc cttgcgctca
    34921 attccgtctc ggagtaccac ggctccttca ccggcatcct cgggaccgga attatgggtg
    34981 gcgcagtgat gcccgctatt attggcagaa ttggcgacca ctttggatta agaaacgggt
    35041 taagccttct ttacctgacc ttcggtttta tcttcagcgt tggcttctgg gcaaagcctc
    35101 tggttaataa tgcgacgctg cgttggaaga gcacagacct ttcagcagtt gtctgaaaag
    35161 tagcgacaaa gaatcagagg ttga
//
LOCUS       DASRBV010000112         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_212299, whole genome shotgun sequence.
ACCESSION   DASRBV010000112 DASRBV010000000
VERSION     DASRBV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212299"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VFA02_04480"
     CDS             <1..131
                     /locus_tag="VFA02_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZU09136.1"
                     /translation="AVTDYGGPFTAAVENANVMGVQFHPEKSGEAGLRILQNFVRL"
     gene            134..913
                     /gene="hisF"
                     /locus_tag="VFA02_04485"
     CDS             134..913
                     /gene="hisF"
                     /locus_tag="VFA02_04485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZU09137.1"
                     /translation="MLTRRIIACLDVDAGRVVKGVQFVDIVDAGDPAELARRHAEAGA
                     DEIVLLDITATHQGRGTLIDTVKRTAKQIFVPFTVGGGIRTANDAAAVFDAGADKVSI
                     NSAAIARPELIEEIGSRFGAQAVIVAIDARRAKESNDPVKDAEVFVSGGRKSTGRKVI
                     EWAREAESRGAGEILLTSMDADGTRAGFDCELTALVSEAVQIPVIASGGAGNAQHFAD
                     VLLAGKADAALAASIFHFGIADVRELKQEIARRGVPVRLPC"
     gene            907..1578
                     /locus_tag="VFA02_04490"
     CDS             907..1578
                     /locus_tag="VFA02_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZU09138.1"
                     /translation="MLIPSIDLMGGRIVQLVRGEKLKLAFDDFEYWIEKFSGYPMVQL
                     IDLDAAMRQGDNAALVEQIARRLPCQVGGGVATIERAKQLIAAGAKRVIFGSALFPSQ
                     INTALAQEIAEKIGTDQFVASIDTKAGRVAIKGWKEQAALTPDEAILALDPYCGAFLY
                     THVDTEGTMQGFPLETAMRLRALTRRQLIVAGGIREQAEIDALDRIGVDAVAGMAVYT
                     GALAT"
     gene            complement(1571..1735)
                     /locus_tag="VFA02_04495"
     CDS             complement(1571..1735)
                     /locus_tag="VFA02_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09139.1"
                     /translation="MRRSSVWFLIAGLWFVITAISTVRRIWPLAALQGVVALVFLLVG
                     VLTRHKETIR"
     gene            1891..>2882
                     /locus_tag="VFA02_04500"
     CDS             1891..>2882
                     /locus_tag="VFA02_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU09140.1"
                     /translation="MTTKKHTLRLAKRLDEVGFSDIVQVRNKVLELRAAGETVHAFHG
                     GEPFFETPDAVKYSMIRALVENKTRYAPSSGIPVLREALAEKLRRRNKLDVTSDNVLI
                     TAGGAHALYVAFQAVLDPGDDMLLFSPYWTPIRDMVTGTQARALLVPTNSARRNGLTK
                     TLEEFATPHTKAIYYNTPQNPTGIVFSRKEAEEVAAFAKKHNLIVIADEAYEDLVYEG
                     EHVSIASLPGMAERTITAYTFSKSYGMTGWRIGYVVAKEPFMTGLRKLVLYSANGVST
                     PTQWAALEALSLPQSYLDDRLKEYRERRDLLVKGLNDLGLECDPPAGAFYAMPNV"
BASE COUNT          653 a          820 c          840 g          569 t
ORIGIN      
        1 tagcagttac agattatggc ggcccgttta ccgcggcggt cgagaacgcc aatgtgatgg
       61 gcgtccaatt ccatccggaa aaatcaggag aagcgggact gcgcattctc cagaacttcg
      121 tgaggctgta gatttgctga ccaggcgcat cattgcatgt cttgacgtag acgcgggccg
      181 cgtcgtcaaa ggcgtgcagt tcgtagacat cgtagacgca ggcgaccctg ccgaactcgc
      241 ccgccgtcac gccgaagctg gcgcagatga aatcgtcctg ctcgacatta ccgcaaccca
      301 ccagggccgc ggtactctca ttgatacagt aaagagaacg gcgaagcaga tcttcgtgcc
      361 ctttactgtc ggcggcggca tccgcacggc aaacgatgca gcagcggtct ttgatgcggg
      421 cgcggacaag gtaagcatca actcggcggc cattgcgcgt cctgaattga ttgaagaaat
      481 cggcagcagg tttggcgcgc aggctgtcat tgtagccata gacgcccgtc gcgcaaagga
      541 aagcaacgat ccagtgaaag atgcagaagt attcgtcagc ggcggacgca agtccacagg
      601 ccgcaaagtc atcgaatggg cgcgggaggc cgagtcgcgc ggagcgggcg aaatcctgct
      661 cacctccatg gacgccgacg gcacgcgtgc aggcttcgat tgcgagctga ccgcgctcgt
      721 gagtgaggcc gtgcagattc ccgtcattgc ttcgggcggc gctggcaacg cgcagcattt
      781 tgcagacgtt ctgctcgcag gtaaagcaga cgcagcgctg gctgccagca tcttccattt
      841 cggaattgcg gatgtccgcg aactaaaaca ggagatcgcc cggcgcggcg ttccggtgag
      901 gctgccatgc tgattccttc cattgatctg atgggcggcc gcatcgtcca gctcgtgcgc
      961 ggcgaaaagc tgaagcttgc ctttgatgat ttcgaatact ggattgagaa attctctggc
     1021 taccccatgg tgcaactgat tgatctggat gctgccatgc gccagggaga caacgctgcg
     1081 ctggtcgaac agattgcgcg ccgcttgccc tgccaggttg gcggtggcgt tgcaacaatt
     1141 gaacgcgcaa agcagttgat tgcagcaggg gcaaagcgtg tcattttcgg ctcagcttta
     1201 tttccttctc agatcaatac tgctcttgcg caggagattg ctgagaaaat cggtactgac
     1261 caatttgtag ccagcattga tacaaaagcg ggccgcgttg ccatcaaagg ctggaaggag
     1321 caggcagcgc tcacgcccga cgaggccatc cttgcgttgg atccatactg cggggccttt
     1381 ctctacacac atgtagacac ggaaggcacg atgcagggct ttcccttgga aacagcaatg
     1441 cggctgcgcg ctctgacccg gcgacagttg attgttgccg gaggcatccg cgagcaggca
     1501 gaaattgacg ctctcgaccg gattggtgtg gacgccgtcg ccggaatggc cgtctacacg
     1561 ggcgcgctgg ctacctgatg gtttctttgt gccgcgtcag caccccaacc agaagaaaca
     1621 ccagtgcaac cactccctgc aaggccgcga gcggccagat gcgtctcact gtggagatag
     1681 cagtaatcac aaaccagaga ccagcaatca gaaaccagac ggacgatcgt cgcatacgca
     1741 gcagaatagc attgtgtatg aatgtcatcc tgacgcgata gcgtgttgct ccatacaacc
     1801 ggaaataccg ctattgaagt agtctcgcct gccatctccc actttcggtt aagctgaaga
     1861 gtcccgctct attccggagg cacgaccgag atgaccacaa aaaaacatac attacgcctg
     1921 gcaaaacggc tggatgaagt cggcttttct gatattgtgc aggtccgcaa taaagtgctc
     1981 gaactgcgcg cagcaggtga gactgtccac gcctttcacg gcggcgagcc attttttgag
     2041 acgccggatg ccgtgaagta ctccatgatt cgcgccctgg tcgagaacaa gaccaggtac
     2101 gcgccgtcct ctggtattcc cgtgctgcgg gaagctctgg ccgaaaagct gcgcaggcgc
     2161 aacaagctcg acgtgaccag cgacaacgtc ctcatcaccg caggcggagc tcacgccctc
     2221 tacgtcgcct ttcaggcggt gctcgatccc ggcgatgaca tgctgctctt ctcgccttac
     2281 tggacgccta tccgcgacat ggtgaccggg acgcaggcgc gggcgctgct ggtgcccaca
     2341 aacagcgccc gccgcaacgg cctcacaaag acgctggaag aatttgccac gccgcataca
     2401 aaggccattt attacaacac gccgcagaac ccgacaggca ttgtgttttc gcgcaaggaa
     2461 gcagaagagg ttgcagcttt tgcaaaaaag cacaatctca tcgtcattgc ggatgaggcc
     2521 tacgaagacc tggtgtatga gggcgagcat gtttcgatcg catcgctgcc cggaatggcc
     2581 gagcgcacca tcactgccta tacattttcc aagagctatg gcatgactgg atggcgcatc
     2641 ggatatgtcg tcgccaaaga gcctttcatg accggactgc gcaagctggt gctgtactcg
     2701 gcaaacggag tttcaactcc aacgcaatgg gcagcgctgg aagcgctctc gctgccgcaa
     2761 agctacctgg acgaccgcct gaaggaatat cgtgagagac gagaccttct ggtaaagggg
     2821 ttgaatgatc tggggctcga atgcgaccct cccgcgggcg cgttttacgc catgcccaat
     2881 gt
//
LOCUS       DASRBV010000113         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_106597, whole genome shotgun sequence.
ACCESSION   DASRBV010000113 DASRBV010000000
VERSION     DASRBV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106597"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..386
                     /locus_tag="VFA02_04505"
     CDS             45..386
                     /locus_tag="VFA02_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09141.1"
                     /translation="MRFSTAGLLLLAALALLGGCASHPAYYVAPPPPPPPAVAYVPPV
                     IQAARQNGYADGIRIGERDRLEGHSFRPTYSEHYANTPGYSPQLGGPFWQYQSEYREA
                     YIRGYRGYAQG"
     gene            420..1175
                     /locus_tag="VFA02_04510"
     CDS             420..1175
                     /locus_tag="VFA02_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HZU09142.1"
                     /translation="MQSRIQGTTMPVLELLLGPNESVISEAGELSWMSSSIQMTTHTQ
                     MAGGGGIFGAIKRVMGGGTLFMTEYRAIGTAGEVAFATKVPGHIVPVEVSGGHEYYIH
                     RHGFLCATPQIQLSVGFQQSLGAGIFGGDGFILQKVSGIGTAWLELSGELIVKDLAPG
                     EMLRVHPGHVGAFQGTVSFQITRIPGIRNMIFGGDGIFLAALTGPGRVWLQTLPISKL
                     AHQLMQYMPQEQARENAETGVVGGIVGSILKGM"
     gene            complement(1156..2286)
                     /gene="tgt"
                     /locus_tag="VFA02_04515"
     CDS             complement(1156..2286)
                     /gene="tgt"
                     /locus_tag="VFA02_04515"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZU09143.1"
                     /translation="MSLKFQLHSTSGNARRATMTLPHGAVETPVFMPVGTVASVKAVP
                     QHLLEELNAQIILSNTYHLYLRPGHELIRRMGGLHRFMSWHRPVLTDSGGFQVFSLNE
                     LRKVTEEGVEFRSHLDGGLHFFSPEHSMDVQIGLGSDIVMAFDECTEYPAEYTRARKS
                     LDLTLRWAERSKRHFDAHKHEVPWGAEDISPGPAPCDLRPSLFGIVQGGMYQDLRREA
                     AERLVEMDFPGYAIGGLSVGEPRELTREIIAETLPLLPKDKPRYVMGVGYPDEIVEYA
                     EMGVDMMDCVLPTRAARHGLLFTSEGRLNIKNRRFAEDQNPPDPECQCMVCGRYSRAY
                     LRHLMASQEPLAAVLNTLHNLAFYLDTMRRLRERLETTSPSE"
     gene            complement(2362..3492)
                     /locus_tag="VFA02_04520"
     CDS             complement(2362..3492)
                     /locus_tag="VFA02_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09144.1"
                     /translation="MFALSTVKTAVRAGGPVKGPQQQAMPDVRSTSVLWPHFSAGSIP
                     VFSKHDGGAGQDSQAESIPAAPTVDSAPQPEPTNPAPGASGTIPDIVTPPYEGKVTEP
                     EISVPKADQAAGRVPVAVNLSMGPLQQPKLDANSAGGKYDPVASGVSMGTFSQPGGRA
                     VSPFGAEFYEPAFSGISWAFSGGKCTISAKLDPVCPWGTNAGGDIDVPSATDPVVTAA
                     TWKAIHDDLQPSATSPFKSPRTKYYSQALVERHEKFHGTDDLGWTTSTGIGFVKTSLE
                     AGSVTAPASAADKARVDTDVANLLNTARTKLIAENLKFYKGAGADHDSFAGEIRAYAD
                     GKAEYQKLADAVEAQGKKLAAPPPVPAPAPGVFPPSPPPPKP"
     gene            complement(3577..4830)
                     /gene="glgC"
                     /locus_tag="VFA02_04525"
     CDS             complement(3577..4830)
                     /gene="glgC"
                     /locus_tag="VFA02_04525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZU09145.1"
                     /translation="MKDTLGVLLAGGAGERLFPLTRDRAKPAVPFAGNYRIIDITLSN
                     CINSGLRKVYILTQYKALSLNRHIREGWTSVVAHELGEFIEILPPMQRVSPNWYMGTA
                     DAVYQNIYSIGSEQPKHVLILSGDHIYKMDYGKMLQQHLESAADVTIATLPIDPSEVS
                     RFGVVEVARSGEVIGFQEKPKSTDLRSPFNPSMVDASMGIYLFNTDVLLPALIKDAED
                     PTSKHDFGHNILPALLGKYKMYAYNFVDENQQDALYWRDVGTLDAYFEANMDVASVSP
                     IFNLYDKNWPMRTRVRQYPPAKFVFGEPGRTGMAINSIVSAGSIISGALVRNSVLSQD
                     VRVNSYTEVDSSIIFSHVNIGRHCRIRRAIIDRDVHIPEGTVIGYDQNEDRKNYFVTP
                     SGLTVVTRDYSLYENPVAADFLQPV"
     gene            complement(4951..5250)
                     /locus_tag="VFA02_04530"
     CDS             complement(4951..5250)
                     /locus_tag="VFA02_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09146.1"
                     /translation="MNANLRKLVQQLGEAIHETVSESEHIAGVVQDIRRQGFEVLLML
                     EATIGLNELEQQPEGALESSNALDQQAESNGTFSSQDVHFLRSLRIAVQDEEQEE"
     gene            5354..>5730
                     /locus_tag="VFA02_04535"
     CDS             5354..>5730
                     /locus_tag="VFA02_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09147.1"
                     /translation="MEPNSVGRKFGIGVRVASKMMIERAARIGEKPAQVNPPGPKKTS
                     VEKPRKPAPLTQVYAHRSKAVAQGARKLTAALWRPVAHASGIIWLQITGLFFSVFAIV
                     FASNTWKLRQNWSAGPGHARFLA"
BASE COUNT         1231 a         1709 c         1574 g         1216 t
ORIGIN      
        1 cctccgtctg ctgttctttt caccatgaca tggaggtgga acaaatgaga ttcagcaccg
       61 cgggacttct tttgcttgcc gctcttgcgc ttcttggcgg atgcgccagc catccggcat
      121 attatgttgc gcctccgcca ccacctccac ctgctgttgc ctatgtcccg cctgtgattc
      181 aggcagcgcg ccagaatggc tacgccgatg gaatccgcat cggcgagcgc gaccggctgg
      241 aaggccacag cttcaggcca acatacagcg agcactacgc caacactccc ggatacagcc
      301 cgcagcttgg tggcccgttc tggcaatatc agtccgaata ccgcgaggcc tacatacgcg
      361 gataccgcgg ctatgcacag ggatgagcgt gtatagtcct tacagaggtt gaacgcacga
      421 tgcaaagccg cattcaaggc accaccatgc ccgtgctcga actgttgctc gggccaaacg
      481 aatccgtcat ctctgaagcc ggcgaacttt cctggatgag cagctccatc cagatgacaa
      541 cgcacacgca gatggccggc ggaggaggca ttttcggagc aatcaagcgc gtcatgggcg
      601 gaggcacgct ctttatgacc gagtaccgcg ccatcggcac agccggcgaa gtcgcctttg
      661 ccacaaaggt ccccggacat atcgttcccg tagaagtcag cggcggacac gaatactaca
      721 ttcatcgcca cggcttcctc tgcgccactc cgcagatcca gctcagtgtc ggctttcagc
      781 aatcactggg cgcgggcatc ttcggcggcg atggattcat tctgcaaaaa gtcagcggca
      841 tcggcacagc ctggctggag ctttccggcg agctgatcgt aaaagacctc gctccgggcg
      901 agatgctgcg cgtgcatccg ggccatgtgg gcgcattcca gggcaccgtt tcgtttcaga
      961 tcacccgcat cccaggcatt cgcaatatga tcttcggcgg cgacggaatc tttctcgccg
     1021 cgctcaccgg gccgggacga gtgtggctgc aaactctgcc catctccaaa ctcgcgcacc
     1081 agctcatgca atacatgccg caggaacagg cgcgggaaaa cgctgaaacc ggagttgtgg
     1141 gaggcatcgt cggttctatt ctgaagggga tgtagtctcc agccgctccc gcagcctgcg
     1201 catcgtgtcc agataaaatg ccagattgtg cagcgtgttc agcaccgcgg ccagcggctc
     1261 ctgggatgcc ataagatggc gcaggtaggc gcggctgtat cgcccgcaga ccatgcattg
     1321 gcattccgga tcaggcggat tctggtcttc agcaaagcga cggttcttga tgttcaggcg
     1381 gccttcggaa gtaaacagca gcccatggcg cgcggcacgc gtgggcagta cgcagtccat
     1441 catgtccact cccatttcgg catattccac aatctcgtcg ggataaccca cacccatcac
     1501 ataccgcggt ttgtctttgg gcagcagggg cagcgtttca gcaatgatct cgcgggtcag
     1561 ttcgcgcggc tcgcccacgc tcaggccgcc tatggcatat ccgggaaaat ccatttcgac
     1621 aagccgctcc gccgcctcgc gccgcaggtc ttgatacatc cctccctgca caatcccgaa
     1681 gagacttggg cgcaggtcac agggcgcagg tcccggggag atgtcttctg ccccccacgg
     1741 cacttcatgc ttgtgcgcat caaagtgacg cttgctgcgt tcggcccagc gcagcgtcag
     1801 gtcaagcgac ttgcgcgcgc gcgtgtactc tgccggatac tccgtgcatt cgtcgaaagc
     1861 catcacaatg tccgaaccaa gcccgatctg cacgtccatc gagtgctccg gcgaaaagaa
     1921 atgcagtccg ccatccagat gcgaacgaaa ctcgacgccc tcctctgtca ctttgcgcag
     1981 ctcattgagg ctaaagacct ggaacccgcc cgaatccgta agaacaggac ggtgccagct
     2041 cataaagcgg tgcaacccgc ccatgcggcg aatcagctcg tgaccgggcc gcaggtagag
     2101 gtggtacgta ttgctcagaa tgatctgggc attcagctct tcaagcagat gctgcggtac
     2161 ggccttcacc gaagccaccg tccccaccgg cataaaaacc ggcgtttcga ccgctccatg
     2221 cggcagagtc atcgtagcgc ggcgagcgtt gccgcttgtg gaatgaagtt ggaatttcaa
     2281 tgacactgct tccagcttaa agcagcgctc ctgcaatcac acaggatgca gaaagggatg
     2341 ccggatcctg aataccttct gttatggctt aggtggaggt ggcgatggag gaaatacacc
     2401 gggcgctggc gcaggcacag gtggaggcgc ggccagcttc tttccctgtg cttctaccgc
     2461 gtctgccagc ttctgatact ccgctttccc gtcagcatag gcgcgaattt ctccggcaaa
     2521 ggaatcgtgg tcggcccccg cccctttata aaatttgagg ttttccgcaa tcagctttgt
     2581 acgcgccgta ttcagaaggt ttgcaacatc cgtgtcaaca cgggccttgt ctgccgcaga
     2641 agcaggggct gttacagaac ccgcctcaag cgaggtcttg acgaatccaa tcccggtact
     2701 tgtcgtccat ccaagatcat ccgttccgtg gaacttttcg tgtcgctcta ccagcgcctg
     2761 agagtaatac ttcgttcgcg gcgatttgaa aggcgaagtg gcgctgggct gaagatcatc
     2821 gtggatggcc ttccaggtag cggcagtcac aaccgggtcg gtcgcagaag gaacatcaat
     2881 gtcgccaccg gcgttcgtcc cccacggaca aaccggatcc agcttcgcgg aaattgtgca
     2941 cttccctccc gagaatgccc agcttatccc gctgaatgcg ggctcataaa actccgcccc
     3001 aaatggcgaa acggcgcggc ctccgggctg gctgaatgtg cccatggaga cccccgaggc
     3061 cacaggatca tatttgcccc ccgccgagtt ggcatccaac tttggctgct gaagcggccc
     3121 catgctgaga ttcacagcaa caggtacccg cccggctgcc tggtccgcct tgggcacaga
     3181 gatttcaggc tcagtgacct ttccttcata cggcggcgtc acgatatcag gaatcgttcc
     3241 ggatgcgcct ggcgccggat tcgtcggctc cggctggggt gcagaatcca ccgtaggcgc
     3301 tgcgggaatg gactcggcct gcgaatcctg ccctgcgcca ccatcatgtt ttgagaagac
     3361 tgggatgctg cctgcactga aatgcggcca caggacagaa gtagaccgca catctggcat
     3421 tgcctgctgc tgtgggccct tcacaggccc tccggcccgg actgcggttt ttacggtgga
     3481 aagagcaaac acaactaccc tcccattcca ttgtgtacgg ggccgtgaag atgtgacaaa
     3541 aatagctctc ctgttctgca attgtcaaaa cagcgcctaa acaggctgca ggaaatccgc
     3601 agctaccgga ttttcataga gcgaatagtc ccgcgtcacg actgtcaggc ctgaaggagt
     3661 aacaaagtaa ttcttgcggt cctcattctg gtcgtagccg atcaccgtgc cctccggaat
     3721 atgcacatcg cgatcaataa tcgcgcgccg gatgcggcag tgccgtccaa tattgacgtg
     3781 agaaaagatg atgctcgaat ccacttccgt gtaggaattt acacgcacat cctgcgaaag
     3841 aacgctgttg cgcaccagtg cgcccgatat gatgctcccg gccgatacga tggagttaat
     3901 cgccatcccc gtgcgccccg gctcgccaaa aacaaacttt gccggcggat actggcgcac
     3961 gcgcgtccgc atcggccagt tcttgtcata cagattgaag ataggcgaga ccgaagccac
     4021 atccatattg gcttcaaaat atgcatccag cgtgcccaca tcgcgccagt aaagagcgtc
     4081 ctgctggttt tcatctacaa agttgtaggc atacatctta tacttgccca gcagcgcggg
     4141 aaggatgttg tgcccgaaat cgtgtttgga cgtagggtct tcggcgtcct taatcagcgc
     4201 gggcagcagc acatctgtgt tgaaaagata aatgcccatc gaagcatcta ccatactggg
     4261 attaaacgga gaccgcaggt ccgtagattt gggcttttcc tggaacccaa tcacctcgcc
     4321 ggagcgcgcc acttcaacga cgccgaagcg ggacacttcc gatggatcaa tcggcagagt
     4381 ggcaatcgta acatcggcag ccgattcaag gtgctgttgc agcatcttgc cgtagtccat
     4441 tttgtagatg tggtcgccag agaggatcag tacatgcttc ggttgctcgg agccaatcga
     4501 atagatgttc tggtagaccg catctgccgt gcccatatac cagtttgggc tgacgcgctg
     4561 catcggcggc aggatctcaa tgaattcgcc cagctcatgc gcgacgacgc tcgtccagcc
     4621 ttcgcggata tgacggttaa gagaaagtgc cttgtactgc gtaaggatat acaccttccg
     4681 caggccggaa ttaatgcagt ttgacagcgt aatatcaatg atccggtaat taccagcaaa
     4741 gggaacggct ggtttcgcgc ggtcgcgggt cagcgggaag aggcgttcac ccgcgccgcc
     4801 tgccaaaagt acgccaagag tgtctttcat ggtttacgac gccctgtttg ctgttgtttg
     4861 ctgctggttt ggtcccggga aacatgaggc ttctgggaac atcacacaaa ctgttattaa
     4921 cctaaaacag ccacagagag caataaacct ttattcctct tgctcttcgt cctgaaccgc
     4981 aatacggagt gagcgcagga agtggacgtc ctgcgaggaa aaagtgccgt tgctttctgc
     5041 ttgctggtca agggcattcg atgactcaag cgcgccctcg ggttgctgtt ccagctcatt
     5101 caggccaatg gtggcttcca gcatgagaag gacttcaaat ccttgtctgc gaatgtcctg
     5161 cacaacgccg gcaatgtgct ccgattccga cacggtttcg tgaatggctt ctcccagttg
     5221 ttgaacgagt tttcgcaggt tggcattcaa ggtgagtgtc tccgtttccg caggcgcgtt
     5281 ggaatagtcc ttacattact tctgatgatt ccgaagggca agggagattc gagcaggctg
     5341 ctaaactttt tttatggagc cgaactcggt cggccgcaaa ttcggaatcg gcgtccgcgt
     5401 cgcatcaaaa atgatgatcg aacgcgccgc gcgcatcggg gaaaaacccg cccaggtcaa
     5461 cccccctggc ccgaaaaaaa catccgtcga aaaaccccgg aagcccgcgc ctttaactca
     5521 agtgtacgcc catcgcagca aggctgtcgc gcagggcgcg cgcaaattga ctgctgcctt
     5581 gtggaggccc gtcgcccatg ccagcggtat catctggctt cagattaccg ggcttttttt
     5641 ctccgtcttc gccatcgttt ttgcctccaa tacctggaaa cttcgccaga actggtccgc
     5701 cgggcctgga cacgcgcgct tcctcgcctt
//
LOCUS       DASRBV010000114         7892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_411215, whole genome shotgun sequence.
ACCESSION   DASRBV010000114 DASRBV010000000
VERSION     DASRBV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411215"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VFA02_04540"
     CDS             <1..1554
                     /locus_tag="VFA02_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09148.1"
                     /translation="LIAGSDGSGLIYRIDGNGKGYVLYDAPKHEITSLAVADNGTIYA
                     SAVGGKGQTTLPPLPVQGQPSVSTTITFMQPGSVQAFNGNTVIPDGSEVYALPPHSAP
                     HKIWSDRDDIVYSLHWTTQGLLAATGNRGRIYRIQDNGDYADIAHIEASQAIGFADSP
                     QGLYIGTANTGKIYLLSHTVAAESTYISDVFDAGVFSRWGRAEVESSASNYELYARTG
                     NIENPERAWSDWKKVTPNNGPLGLEQARFLQWKLVLHPGASIGSVGINYLPVNVAPVI
                     DEIAVVPGARVTAPPQQQQNQPITISFNEPSRPTVQFNEPGHEPLTGIKDKSAVTVRW
                     AAHDDNGDELIYTLYYRGDGDKETNWQLLKDKITERFYSFDATTFPDGAYRLKVVASD
                     APSHNPGEALTTEKVSDRFILDTTPPSLSPIDAHMVNGKIHVNLTATDATSPISHAEY
                     SIDAGPWQYVEPVGKLSDSLTEHYDFDAVIPPHQPGPGAPETPSEHLITVRAYDRYDN
                     VAAAKAVVR"
     gene            1551..2807
                     /locus_tag="VFA02_04545"
     CDS             1551..2807
                     /locus_tag="VFA02_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU09149.1"
                     /translation="MRFELFVAARYLRAKRRQAVIGVITAISVIGVAVGVAALIIALA
                     ITNGMSRDLRERLLSSTAHVSLMRIQSDGIRDWRTLTDRLRKLPHVTAAAPGLYEPVL
                     ISRGALSGGAEIKGIIPSEENTVSDLLSRITQGSAAPLSKPEPPASTDHANRPTPPIV
                     VGYDLADSLNARVGDTVMVTSPQGELTPYGLVPKYQRFKIVGIFHSGFYQYDSSFAFV
                     RLEDAQRLFDEPDLVSIISFKVDDLYHADRIGKEIEQAAGPGFETTNWMEQNRELFRA
                     LKLEQIVTFIVIGLIVCVAALNILIALTMMVMEKTKDIAVLMSIGVRPEQVRRIFLMQ
                     GFLISLTGTIIGLLLGYALSWAGGHYRFIHLSADVYSIDYLPFAPRIRDGIIVALVSL
                     GVSLLATLYPSSSAARVLPAEALRYE"
     gene            2870..4249
                     /locus_tag="VFA02_04550"
     CDS             2870..4249
                     /locus_tag="VFA02_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZU09150.1"
                     /translation="MNCHPGPGETKWSEAGGRRTWFSSPDVGYSIILARTIVLLVLTI
                     LLPTQASSAYSVLTHQQIIDLAWEPAIKPLLLARYPSTTEAQLRVAQSYAYGGCEIQD
                     AGYYPFGHVFFSNLTHYVRTGDFVASLIRNARNVNELAFALGALSHYIGDSIGHQDAI
                     NPATAIAFPKLALQYGHSITYDESPHGHVRTEFAFDVDEMTKHHFAPASYLDHIGLRV
                     SPGLLERAFYETYGLHLSEVLGNKRRTAVIASYRHSVRSFLPAFAHAEAVVHQHDFPA
                     DLNTEEFQKLDTLIQQTSNQNGWDHMRRGPGLKTHLLAFVVILVPKVGPAQDLAIKIP
                     TQETETKYVASVNHTLDLYEKRLNDLRANPAQTPEAILDLENRDLDTGYIVQPGGYPR
                     TDKTYAQLLGSVTKYHDRPVPSGLKHDILAYYSNPDSPIITKKNRKAWQRVQDEIALL
                     QTMPVVPLK"
     gene            4494..5288
                     /locus_tag="VFA02_04555"
     CDS             4494..5288
                     /locus_tag="VFA02_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU09151.1"
                     /translation="MGTPLTSSHVLSAGPVPAGLAPLSPWTASEHPYVRVENLRKEYA
                     TGRGRLVLFDGLSFEIRKGEFLAIVGQSGAGKSTLLHILGALDVPTSGNVYCASTPLR
                     NLSPGQAAAFRNREVGYVWQFHYLLPEFTALENVAMPLLARGETRAEAFRRARGCLRE
                     VGLEDRAEHQAGELSGGEQQRVSLARALVTQPGLLLADEPTGDLDSQTADTVFSLLQR
                     LHEKEHLTSVIVTHNMTLARRCHRVLRLEKGRMEELSPQEIQRRVE"
     gene            5473..>7892
                     /locus_tag="VFA02_04560"
     CDS             5473..>7892
                     /locus_tag="VFA02_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZU09152.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     TNRFLRSHASVDSIRKQIEGHTTIREKVSTSVDLPLSNECKRVLAYAAEEAERLSHKH
                     IGTEHLLLGLLREEKSFAAEILHERGLKLIAIREELARTTQEKAPQQQRNRESSLLAE
                     FSRDLTQAALDGQLDPLVGRDSELERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIADGDVPSFLSDKRVLALDLSLIVAGTKYRGQFEERLKTIMKELMENQNCIIFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPGEYRKSIEKDRSLERRFQAVKV
                     PPPNEDDAVKIIMGIKDRYEKFHAVSYTDDSIQFAVSHSNRYISDRYLPDKAIDLIDE
                     AGARVKLRQTSLPDEITEVHKRIKFIVHRMDSAIANHEFEKARFYSDEERKERENLRA
                     LRDKYHLDDSAAGIVTREDIEEVVSRWTGVPVTSIKEEETQKLLRVEEELHKRVISQD
                     KAISALARAIRRSRAGLKSPHRPIGSFLFLGPTGVGKTEMARTLAQFLFGSEKALIRF
                     DMSEFMEKHSVSKLIGSPPGYVGYEEGGQLTERVKRSPYSVVLLDEIEKAHPDVFNIL
                     LQVFEDGQLTDGLGNTVDFKNTIIIMTSNIGARHLQRKQGLGFQSDREELVMEKIEEL
                     VKNEVKRTFNPEFLNRLDEIIIFQALTDADLIQILELLVQQLNTNLAQKAITISVTDE
                     AKKWILEKTVVDRTYGARPLRRAIQRYVEDPLSEALIAGQISERPAFIEVYMDNNQLF
                     YRPVGKEG"
BASE COUNT         1815 a         2366 c         2066 g         1645 t
ORIGIN      
        1 ctcatcgcgg gttcggacgg aagcggtctt atctaccgca tagatggcaa tggcaagggc
       61 tacgtccttt acgacgcgcc gaagcacgaa attacgtctc tcgccgttgc cgataacggc
      121 accatctacg cctcggccgt cggcggcaag ggacaaacca cgcttccccc gctacctgtg
      181 caggggcaac cttcagtatc caccacgatt acattcatgc aacccggatc tgtgcaggca
      241 ttcaatggca acactgtcat tcccgatggt tcagaggtct acgcccttcc gccacactct
      301 gctccacaca aaatctggtc agaccgagat gacattgtct actcgctcca ctggacaacg
      361 caaggtctgc ttgccgccac aggaaatcgc ggtcgtatat accgcattca ggacaatggc
      421 gattacgcag acatcgcgca cattgaagca tcacaggcca tcggctttgc cgactcacct
      481 cagggcctat acataggtac cgcaaacaca gggaaaattt atctgctgag ccataccgta
      541 gctgcggaaa gcacctatat cagcgacgtc tttgatgctg gcgtcttctc ccggtggggg
      601 cgggccgagg tggaatccag cgcctccaat tatgaactgt atgcgcgcac aggaaatatc
      661 gaaaatcctg agcgtgcatg gagtgactgg aagaaggtca caccgaacaa tggcccgctt
      721 gggcttgaac aggcccgttt tctccaatgg aagctcgtcc ttcatccggg tgcaagcatt
      781 ggttctgttg gaattaacta tcttcctgtc aatgtggcgc ccgttattga cgagatcgcg
      841 gtggttcccg gagcgcgggt gactgctcct ccgcaacagc agcaaaacca gccaattacc
      901 attagcttta acgaaccatc gcgtccaacg gtgcagttca atgagccggg acacgaaccc
      961 cttacaggaa tcaaggacaa atccgctgta accgttcgct gggccgcaca cgatgataac
     1021 ggcgacgagc ttatttacac cctctattac cgcggagacg gcgataagga aaccaactgg
     1081 cagctgctca aggataagat caccgagcgc ttttacagtt tcgacgccac gaccttcccc
     1141 gacggcgcat accgtctgaa agttgtggcc agcgatgcgc cctcacacaa tcccggcgaa
     1201 gcgctgacga cggaaaaagt cagtgaccgc ttcatccttg atacaactcc gccatcgctc
     1261 tcgcccattg acgcgcacat ggtgaacgga aagatccacg tcaacttaac ggcaacagac
     1321 gcaaccagcc ccatctcaca cgcagaatat tccatagatg caggcccgtg gcaatacgtc
     1381 gagcctgtcg gaaagctgtc cgattccctc accgagcact atgactttga cgcagtgatt
     1441 ccgccacatc agcctggacc aggcgcacca gaaactccat ccgagcacct gattacggtg
     1501 cgtgcctatg accgctatga caatgtggct gcagcaaagg cagtcgtccg gtgaggttcg
     1561 agctatttgt cgccgcgcgc tacctgcggg ccaaacgtcg gcaggcagtc atcggcgtca
     1621 tcacagccat ctccgtcatt ggggtcgctg tcggtgtggc tgcactcatc attgcgctgg
     1681 ccatcaccaa tggcatgagc cgtgacctgc gcgaaagatt gctcagttcc accgcgcacg
     1741 tttcgctcat gcgtatccag agcgacggta tccgtgactg gagaacactc accgatcgtc
     1801 tgcgcaagct gccgcacgtc actgcggccg ctcccggcct ctacgagcct gtgcttatct
     1861 cgcgcggcgc tctctccggc ggagcagaaa tcaaaggcat catccccagc gaagaaaaca
     1921 ccgtaagcga cctgctctcg cgcatcacgc agggcagcgc tgcgccgtta tcaaagccgg
     1981 agccgcccgc atccaccgat catgccaacc gcccgactcc gcctattgtt gtcggatacg
     2041 atctcgccga ctcgctcaac gccagggttg gtgacacggt catggtcacc agcccgcagg
     2101 gcgaacttac gccttatgga ctcgtcccca aataccagcg ctttaagatc gttggcattt
     2161 ttcattccgg cttctatcag tacgactcta gcttcgcctt cgtacgcctc gaagacgcgc
     2221 agcgcctttt tgacgaaccg gacctggtct ccatcatttc cttcaaagtg gacgacctct
     2281 accacgctga ccgcattggt aaagaaatcg aacaggccgc cggccctggc tttgaaacta
     2341 ctaactggat ggagcagaac cgcgaactct ttcgcgccct caagctcgag caaattgtga
     2401 cctttatcgt cattggcctc atcgtctgcg ttgccgcgct taacatcctt atcgcgctca
     2461 ccatgatggt gatggaaaaa accaaagaca tcgccgtgct catgtccatc ggtgtcagac
     2521 cagaacaggt ccgccgcatc tttctgatgc aagggtttct catcagcctg accgggacca
     2581 tcatcgggct gctcctcgga tatgccctct cctgggccgg aggccattac cggttcatcc
     2641 atctgtcggc agatgtctac tcgattgact atttaccgtt tgccccgcgc atccgcgatg
     2701 gcatcatcgt tgccctggtt tcgctcgggg tctcgctgct ggccaccctc tatccttcat
     2761 cctcagcagc gcgcgtcctg cccgcagagg ccctccgcta cgaatagagc aagcaaccag
     2821 ctatgatttt tgcgagtcga acaatcgaat gtttgcacgc cactatcaaa tgaattgtca
     2881 tcctgggcca ggcgaaacga agtggagcga agccggagga cggaggacct ggttttcttc
     2941 gccggatgtc gggtattcca taattctggc ccggacaatt gtcctcctag tgctgacgat
     3001 ccttctcccc acccaggcct cctcagctta ctccgttctc acccatcaac agatcattga
     3061 tcttgcatgg gagccagcca tcaaacccct gctgctggcg cgttacccca gcactactga
     3121 agcccagtta cgcgtggccc agtcttacgc ctatggcggc tgcgaaattc aagatgcggg
     3181 ctattatccg tttggccacg ttttcttcag caatctcacg cattacgtcc gcaccggaga
     3241 cttcgtcgcc agccttattc gcaatgccag aaacgtaaat gagctggcct tcgccctcgg
     3301 cgcgctttcg cactacatag gagacagcat cggccatcag gacgctatca atcctgccac
     3361 ggccatagca ttcccaaagc tggccctcca gtatggccac tccatcacct acgatgaaag
     3421 cccgcacggc cacgttcgca ctgaatttgc ctttgatgtg gacgagatga ccaagcacca
     3481 ctttgcaccc gcctcttatc tcgatcatat tgggctgcgc gtctcacccg gccttctgga
     3541 acgcgcgttt tatgaaacgt atggtcttca cctgagcgag gtcctcggca acaagcgccg
     3601 gacagccgtc attgcctcgt atcgccactc ggtccgcagt tttctgccag cctttgctca
     3661 tgcagaggcg gtcgttcatc agcacgactt ccctgccgat ctgaacacag aagaatttca
     3721 aaaactcgac acgttgatcc agcagacaag caaccaaaac ggatgggacc acatgcgccg
     3781 cggaccgggt ctcaaaactc acctgctggc ctttgttgtc atcctggtgc ccaaagtcgg
     3841 cccagcccag gaccttgcca tcaaaattcc gacgcaggaa acagaaacca agtacgtggc
     3901 aagcgtaaac cacacgcttg atctttatga aaagcggctg aatgacctgc gcgccaatcc
     3961 tgcgcaaaca ccagaagcca tcctggacct cgaaaaccgt gatctcgaca caggctacat
     4021 tgtccaaccc ggcggctacc cgcgcaccga taaaacctac gctcaactcc tgggcagcgt
     4081 cacaaagtac catgaccgcc cggtgccctc aggactaaag catgacattc tcgcctacta
     4141 ttccaatcct gactcgccca tcatcactaa gaaaaaccgg aaagcatggc agcgcgtgca
     4201 ggatgagatc gctcttcttc aaacaatgcc tgtcgttcct cttaagtagt ttctgtggcg
     4261 caggcggagg acttgttacg gtaaaaaatg tttcagcgtt ccaacaactg ctctcctgca
     4321 gcaagccaaa agattcaaaa tctttacaga aatgtttggc atatcaactg caagcattcc
     4381 agtaaaagga agcaatgtca tgagcgccgc ttgttgaatc taaatagtca gagaggaaaa
     4441 aatctggtgc gtcaagtctt tctttttggt gtccaacaat gatagaggta gttatgggaa
     4501 cccccttaac cagttcgcat gtcctttcag ccggcccagt accggcaggg ctggctccgt
     4561 tgagtccgtg gactgcctct gagcatccat acgttagagt agaaaatctg cgtaaagagt
     4621 acgcaaccgg acgcgggcgg ctggttttgt ttgacggcct tagttttgag atccggaagg
     4681 gggagtttct ggccatcgtg ggccagtccg gcgcaggaaa gagtacgctt ttgcacattc
     4741 ttggcgctct tgatgttcct acgagcggta acgtatactg cgcctcaact ccattgagaa
     4801 atctatctcc aggccaggcc gcagctttcc ggaaccggga agtcggctat gtgtggcagt
     4861 ttcattatct tttgcctgaa tttaccgctt tggaaaacgt ggctatgccg cttctggcgc
     4921 ggggcgaaac gagagctgag gcgttccgga gggcaagagg atgcctgcgc gaggtgggat
     4981 tagaagaccg ggcggaacat caggcgggag agctctcggg aggcgagcag cagcgtgtgt
     5041 cccttgcccg cgcattggta acacagccgg gattacttct tgctgatgag ccgacgggcg
     5101 atctggacag tcaaactgct gatacggttt tttcgctgct ccagcgtttg cacgaaaaag
     5161 aacatttgac atcggtgatt gtaacgcaca atatgacgct ggcacggcga tgtcaccggg
     5221 ttttgcggct ggaaaagggc cgcatggaag aactatctcc gcaggaaatc cagcggagag
     5281 ttgagtagta ccgaggtggg atggccgcca agaaggcgtc catcccaaag gccgtcttca
     5341 ccgggccagt tttcgatggg tgaaagcggc gagaagggcc gaaggcgtat gtgctttgcg
     5401 aaggagtcct cgtagcagaa ggacaccgaa aaaccgggct tcgacaaacg cagacgtctt
     5461 ctcaggggaa atatgttcga acgctataca gaaaaagcac gtcgcgtcat cttctttgca
     5521 agatacgagg ccagtcagtt tggctcgccc tatattgaga ccgagcacct gctgctcggc
     5581 ctgttgcgtg aggacaaggc gctgaccaac cgctttctcc gctcgcacgc ttctgtggac
     5641 tccatccgta agcagattga gggccacacc accatccgcg agaaggtatc cacttccgtt
     5701 gatctgccgc tctccaatga atgcaagcgc gtgctggcct acgcggctga agaagctgag
     5761 cgcctctcgc acaagcacat tggcactgaa caccttctgc tcggccttct gcgtgaagaa
     5821 aagagcttcg ctgccgaaat cctgcatgag cgtggactca agctcattgc cattcgcgag
     5881 gagctggccc gcacgacgca ggaaaaagcc ccgcaacagc agcgcaaccg ggaatccagc
     5941 ctgctggcag aattctcgcg cgatctgacc caggcggcgc tcgatggcca gcttgatccg
     6001 ctggtagggc gcgattctga actggagcgc gtcgtccaga ttctctgccg ccgtacgaag
     6061 aacaatccag tgctcatcgg cgaacccggc gtcggcaaga ccgcgattgt cgaagggctg
     6121 gcccagcgca ttgctgacgg agatgttccc agcttccttt ccgataagag ggtgcttgct
     6181 cttgatctct cgctgatcgt cgccggtacc aagtatcgcg gccagtttga ggagcggctg
     6241 aagaccatca tgaaagagct gatggagaac cagaactgca tcatcttcat tgatgagctg
     6301 catactctgg tcggcgccgg ttctgctgaa ggctcgcttg acgcggccaa tattctcaag
     6361 cctgcccttt cacgtggcga aatccagtgc atcggcgcaa ccacgccggg tgaataccgc
     6421 aaatccatcg agaaagaccg ctcgttggaa cggcgcttcc aggccgtcaa ggtaccgccg
     6481 ccaaacgaag acgatgcggt caaaatcatc atgggcatta aagaccgcta tgagaagttc
     6541 catgcggtca gctacaccga tgactcgatc cagtttgcgg tctcgcactc aaaccgttat
     6601 atctcagacc gttatctgcc cgacaaggcc attgatctga ttgacgaggc gggagcacgt
     6661 gtcaagctgc ggcagacttc cctgcccgac gaaatcacgg aagtccataa gcggatcaag
     6721 ttcatcgtcc accgtatgga ttcggccatc gccaaccacg agttcgagaa ggcccgcttc
     6781 tactccgatg aagagcgtaa ggagcgcgaa aacctgcgcg ccctgcgcga caaataccac
     6841 ctggacgact cggccgccgg cattgtcacc cgggaggaca ttgaagaggt ggtcagccgc
     6901 tggacgggtg ttcctgtcac atccatcaag gaagaagaga cccagaagct gctgcgtgtc
     6961 gaagaagagc tgcacaagcg cgtcatttcg caggacaagg ccatttcagc cctggcgcgg
     7021 gccatccgcc gttcgcgtgc cggactcaaa tcgccgcacc gacccattgg ctcgttcctc
     7081 tttctcggtc ccacgggtgt tggcaaaacg gaaatggcgc gtactctggc ccagttcctc
     7141 ttcggctcag agaaggcgct tatccgcttc gatatgtcgg agttcatgga gaagcactcg
     7201 gtctccaaac tcatcggctc gcctccggga tatgtgggat atgaggaggg cggccaactc
     7261 accgagcgcg tgaagcgttc gccgtactct gtcgtcctgc tcgatgaaat cgagaaggcg
     7321 catccggatg tcttcaacat cctgttgcag gtctttgaag atggtcagct cacggatggt
     7381 ctcggcaaca ccgtagactt caagaacacc atcatcatca tgacctcgaa tatcggggcg
     7441 cgccatctcc agcgcaagca agggctcggc ttccagagcg accgtgaaga gctggtgatg
     7501 gagaagatcg aagaactcgt gaagaacgag gtaaagcgta ccttcaaccc cgagttcctg
     7561 aaccgtctgg acgaaatcat tatcttccag gcgctcacgg atgcggacct catccagatt
     7621 ctcgagttgc tggtccagca gctcaacacg aacctggcgc agaaggccat cactatttca
     7681 gtcaccgacg aggccaagaa gtggatcctc gaaaagaccg tggtagaccg cacgtacgga
     7741 gcacgtccgc tacgccgcgc gatccagcgc tacgtggaag acccactctc cgaggccctg
     7801 attgccggac agatcagcga acgtccggcc ttcatcgagg tctacatgga caacaaccag
     7861 ctcttctacc gcccagtggg caaggaaggc ga
//
LOCUS       DASRBV010000115         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_563669, whole genome shotgun sequence.
ACCESSION   DASRBV010000115 DASRBV010000000
VERSION     DASRBV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563669"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFA02_04565"
     CDS             <1..632
                     /locus_tag="VFA02_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU09153.1"
                     /translation="ITCMVNKSRESFSTMLEIFGPDALLWRSVNRIMATTLATVEARE
                     VLRCEHCNLVQFRTSNSLCRRCHKPLEIEDPGPLFPQITPDAEQAAEGDGLQVARAVR
                     DMRRERRLSQRQLAGRMGVPRTYISKIENGKAVPTLSSLERLAHALEVDIRVLLQDAR
                     SRRYEEMTAIMADSFLREIAPYISRLDAYQRSIFLNQVRDMAMGRRKTA"
     gene            785..2206
                     /locus_tag="VFA02_04570"
     CDS             785..2206
                     /locus_tag="VFA02_04570"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HZU09154.1"
                     /translation="MTFALAAGMVLTGSSRLLAQDVPPPPPDAQDQQQPQDNTGGDQQ
                     VPQYGAPLPSNNSQQQQPQDQQTAPAQQQQAQPLTADQLDQLVAPIALYPDALVAQIL
                     AASTYPTQVVEADQWRQAQGNASPDQIASAANSQPWDPSVKALTAFPSVLKQMHDNMQ
                     WTTDLGNAYYNQPQDVMDAVQRMRQRAQQAGTLADTPQQTVAEDDGNIEIEPTNPDVV
                     YVPVYNPWVVYGPPIIAWPGYYYAPPPAFFVGVGFGWGFRMGFAWGVPLRPWAPWGWG
                     WGHWGCGWYNRTVVYNRTTYITRSTTVINRGVNRPGSPPLQLAGTRGSFVNRPQFANY
                     AHNVAMGVRPAPVASRPGMVSPGNRVINPGGPMRPAPSTSGFMNGNRPTPQNMPRPTY
                     SSPAPYRPSAPQMPTYSRPAPAYRAPQSFGNPAPVYRAPQNYHSVPAPHYSAPAPRIS
                     APAPHFSAPPAAHFSGGGGFHHR"
     gene            2306..>2676
                     /gene="uppS"
                     /locus_tag="VFA02_04575"
     CDS             2306..>2676
                     /gene="uppS"
                     /locus_tag="VFA02_04575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZU09155.1"
                     /translation="MAIYRRLDPERLPKHVAIIMDGNGRWAGKRSLKRFLGHQQGAEA
                     VQYVVETASRIDLPWLTLYAFSLENNLRRPKTEVNFLMRLLSTYLNNNVKRMNDNNVR
                     IQYIGRTHELPSEVQERMQWAA"
BASE COUNT          591 a          801 c          740 g          544 t
ORIGIN      
        1 acattacctg catggtaaac aagagccgcg aatctttcag tacaatgctg gagattttcg
       61 gcccggacgc cctgctctgg cgctccgtaa acagaattat ggcaactacc ttagcaaccg
      121 ttgaggcacg cgaggtactc cgttgcgaac actgcaacct ggtgcaattc cgcacttcga
      181 attcactatg cagacggtgt cataaacccc tcgaaatcga agaccctgga ccgctctttc
      241 cgcagattac gccggatgca gaacaggcag cggaaggtga tggtcttcag gtagcacgcg
      301 ccgtgcgtga tatgcgtcgt gagcgcaggc taagccagcg ccagcttgcg ggcagaatgg
      361 gagtgccgcg cacttacatc tcgaaaattg agaatggcaa ggccgtaccc actctgtctt
      421 cgcttgaacg gttggcccat gcacttgaag tggacattcg tgttctgctg caggatgcac
      481 gcagccgcag atatgaagag atgacggcca tcatggctga ttccttcctg cgggaaattg
      541 cgccatacat cagccgcctg gatgcatacc agcgttccat ttttctgaac caggtacgcg
      601 acatggccat gggacgcaga aagacggctt aagtctttct taaggaaaac tatacacggg
      661 gattggggtt cttatatctg ggcgacaatt ccaccggaat tgccgtatgg tgagaacgat
      721 gttccccaaa tggaaaaccc gaacgaacca tgattcccag cgtgtacgca gtttgcgttt
      781 atggttgacg tttgcccttg ccgccggaat ggttctgacc ggaagctcaa ggctgttagc
      841 acaggatgtg cctcctccgc cgccggatgc acaggaccag cagcaaccgc aagacaatac
      901 tggcggcgac cagcaggtcc cccaatatgg tgcgccgctg ccgtccaaca actcgcagca
      961 acaacagcca caagaccagc agacggcccc cgcgcagcag caacaggcac agccgttgac
     1021 ggccgaccag ctcgaccagc tagtagcgcc gattgcgctc tatcctgacg ctctggtggc
     1081 gcagattctg gctgcttcta cgtatccaac ccaggtggtt gaggccgacc agtggcgtca
     1141 ggcacagggc aatgcttccc ctgaccagat tgcctctgcc gccaattcgc aaccctggga
     1201 cccaagcgtg aaggctttga ctgcgtttcc ttccgtgctc aaacagatgc acgacaacat
     1261 gcaatggacg acggacctgg gcaacgcgta ttacaaccag ccgcaggacg tgatggacgc
     1321 cgtgcagcgt atgcgccagc gcgcgcagca ggcaggcacc ctggcggaca ctccccagca
     1381 gactgtggct gaagacgatg gcaatatcga gatcgaacca acaaatcctg atgtcgtata
     1441 cgtgcctgtc tacaatccat gggtcgttta tggacctccg attatcgcct ggcctggcta
     1501 ctactacgct cccccaccag cattcttcgt aggcgtaggc tttggctggg gcttccgcat
     1561 gggctttgcc tggggcgttc ctctgcgccc gtgggcgccg tggggatggg gatggggcca
     1621 ctggggttgc ggctggtata accgcaccgt agtctacaac cggacgactt acattactcg
     1681 aagcacaacc gtcatcaatc gcggcgtaaa ccgtcccggc tcgcctccgc tgcaactggc
     1741 cggaacacgc ggatcgtttg tgaatcgccc acagtttgca aactatgcgc ataacgtagc
     1801 gatgggcgtg cggccagctc ctgtggccag ccggcccgga atggtgtcgc ccgggaaccg
     1861 tgtgatcaat cctggtggcc cgatgcggcc agctccgtca accagcggct ttatgaacgg
     1921 taatcgtcct acgccgcaaa atatgccgcg gccaacatat tcttcgcctg ctccgtaccg
     1981 gccttcggcc ccccagatgc caacgtacag tcgtccggcc ccggcttaca gagcaccgca
     2041 gagctttggc aatccagccc cggtatatcg cgcgcctcag aactatcatt ctgtacccgc
     2101 gccacattac agcgctcctg caccacgtat aagtgctccc gcgcctcatt tcagcgcacc
     2161 tcctgctgcg cacttcagcg gaggtggtgg gttccatcac agataggaat gaaaggacgt
     2221 gcctgattgc agcaggcacg tccatgtgct acggtcggat gagaggataa ttttgagccg
     2281 tattcatgag ctaccgccgg aagagctggc gatctatcgc cgtctcgacc ctgagcgcct
     2341 cccgaagcac gtggccatca tcatggacgg gaatgggcgc tgggcgggga aacgcagcct
     2401 caagcgtttt ctcgggcacc agcagggagc agaggcagtc cagtacgtgg ttgagactgc
     2461 ttcacgcatt gatctgccgt ggcttacgct ctatgccttt tcgcttgaaa ataatctccg
     2521 gcggcctaaa acagaagtca atttcctcat gcgcctgttg agcacttacc tcaacaacaa
     2581 tgtaaagcgt atgaatgaca acaatgttcg tattcagtac attggccgaa cccatgagct
     2641 gcccagtgaa gtgcaggagc gcatgcagtg ggccgc
//
LOCUS       DASRBV010000116        12912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_718426, whole genome shotgun sequence.
ACCESSION   DASRBV010000116 DASRBV010000000
VERSION     DASRBV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718426"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1080
                     /locus_tag="VFA02_04580"
     CDS             16..1080
                     /locus_tag="VFA02_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZU09156.1"
                     /translation="MSSNGFSSRSSRSHGLRSLFSMFSSDLAIDLGTANTLVYARGKG
                     IVVNEPSIVAINKNTGEVEAVGKEAKEMLGRTPGNIVAIKPMKDGVIADFKVTEKMLN
                     YFIQKAHNRKMLVHPRIVIGVPSEITQVEKRAVEDSAYRAKASEVFLVEQAMVAAIGA
                     GLPITEPSGNMVVDIGGGTTDIAVISLAGIVYSRSVRMAGNQMDEAIMNYLKRKYNLL
                     IGERTAEQIKLEIGSAYPLEKPLTMEIKGRNLIEGVPKTITIDDGEIREALGECIATI
                     MNAIRVALERTPPELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIADDPLASV
                     VLGTGKMLSDFRLLRKIKID"
     gene            1129..2778
                     /gene="mreC"
                     /locus_tag="VFA02_04585"
     CDS             1129..2778
                     /gene="mreC"
                     /locus_tag="VFA02_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZU09157.1"
                     /translation="MESFFSRYKNALVLILVLLVQVIGVAIQVRRPLPREHAQPDAGG
                     VRLIRYWAISLVSPPERLVHWIGNGISGLWSNYIDLIHVRQQNKDLQAELRRLRIEQG
                     GVAEDARQGQRLQQLLAFTEKYIYKTVPAQIIGTSGLDQSRILYIDKGAKDGVAVDMP
                     VITPDGIVGRVREVFPHSAQVLEISDPTSGAGVILADTRIRGILRGNALGQPVIVGIM
                     PDERIKPGEKVVTSGGDQIFPRGLAVGVVDKVVPDPERDPMVDIVIRPAADLSRLEEV
                     LVITDMGSQITPKEKLDLMQSQAIADSEQLKASEILAERLPSRIDPDNPEAAKDTDTV
                     GADGEIARPIQPPPPLHPDHFSPNATPPAASLVPGARAAAVLQDTANKTTAGVPKPRP
                     AVPANGAGSTAPVTASTATAAPPRQKSPELVPDDGSRPPATAVQRAPLATQPTAGNGQ
                     LRRSVSDSTPVAAPSALPKTIIVTDGPRKMTPPAPATQKAPGAATTTPQKPPAQQHKG
                     PELVPDDGSRPPESMLHKPVKPQTQPQTQTAPQQPAGGPQR"
     gene            2775..3299
                     /gene="mreD"
                     /locus_tag="VFA02_04590"
     CDS             2775..3299
                     /gene="mreD"
                     /locus_tag="VFA02_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZU09158.1"
                     /translation="MSMLSAAVRRDSESQSHPLLVYVLAPLLALGLQSFVALHFHGFA
                     ILDLPLLVTIYFAISHRHPVAGTVTGALIGIAQDALTRHPLGIFGICKSIIGYLAASL
                     GVRIDTENPATRAALTFCFVFLHSGIYWLLVRRLLAQPFLWIWLHELLRAVVNAVVAV
                     VLFALLDKTRRKEY"
     gene            3350..4903
                     /locus_tag="VFA02_04595"
     CDS             3350..4903
                     /locus_tag="VFA02_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZU09159.1"
                     /translation="MKVRLFFEGAGIAILAFIAAVWVFLLPDHLVIYHNPRPLSTIAG
                     ALALDLFFVTILFFAVFIFLDHLGPAKTRIAWAAIAAWVLTRLVNITIFFLNYYDAEN
                     VPGRKQKSLLQFAIFGGVFLLWWLGREKFQAFVRAVRMGLALTGCCIVWMLPQLSVLA
                     LHTPAKEISTFTNPNITAVPPSSERIVWILFDELSYDQVFDHRQPDVLLPHFDQLRDE
                     SVAFANVQPAGYFTDIIVPSLFLGRELKGIRSSIEGDLYVRPAQTKHWEPFNPYATVF
                     YDAQQSGWSTGVAGWFNPYCRILSGVLDSCYWESINPFEHGLSDQKSTLANAMSFFKG
                     VGISRITGPASAERSAAEVHAQEYNNVLPAATALIRNDAIRFAFVHLPVPHPPGMYDR
                     HTHTFSKRGTYLDNLVLADQALGTLLDALARTADASHTVLIVSSDHSWRVGMWKMNNV
                     AWTDEEEHATGGKFDSRPVLLVHFPGENHGETRQRPFPEIRERALIDALLQGKLRSQS
                     DLDQWPVPK"
     gene            complement(4989..5612)
                     /locus_tag="VFA02_04600"
     CDS             complement(4989..5612)
                     /locus_tag="VFA02_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09160.1"
                     /translation="MRSLGILALGVFLVTGCSRHPSQPPPPAQQPQQAAENAIGVLQK
                     LVNEQNYKSLGFDSLDEVKRAQLATPIQVYNIGLDKLKGYQAGQDPNTLLAPSPETIY
                     PVTVDGQVKTGVMIVHKEQGYEPSGFGNADIVKRLAGNRHNEGEFAVRIPAFNMYFVG
                     RRVEDRLVLVPIVNDPRLKVEQGQAVPIEVVLDQLKPYVDAYNGLPM"
     gene            complement(5614..6201)
                     /locus_tag="VFA02_04605"
     CDS             complement(5614..6201)
                     /locus_tag="VFA02_04605"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HZU09161.1"
                     /translation="MWKAMQAVCVLALSGFLAGCCTPGNIGSVPVTLNGQQTNMWCWA
                     ASGQMTMNYIHPGSNVQQCDEANKEFGRSDCCNSPVPSACVNGGWPQYDKYSFKADQT
                     SDAPLTWAQIQSQIYCAKKPFAFSWHWNGGGGHMMVATGYVTIDGTNYVSVNNPWPPT
                     GGVQEVYTYDKYVGGASWDHTHWNDFYNITYTGGQ"
     gene            6346..8403
                     /locus_tag="VFA02_04610"
     CDS             6346..8403
                     /locus_tag="VFA02_04610"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09162.1"
                     /translation="MRTTRRLLRQRRWKKRAVFAQPDRNLKERTRAFCEASTLAAQQR
                     HIIALFSYIWNRPSDRGFAFVLLRWIRLLEQDRPLRAQFERSWITLLSGLDAVSVFSD
                     TGLPAHHALLPEAFRRIFQRLLPSAREESDMARLFTTVFPSPRAIGRFVQLDRTTFHR
                     LARVLWPKGGLDALPRLRDDLRQSLILLATRIAGRGASAPIRKRSSIASAEESSFYKL
                     VFATEAFVQTVEESPHTALHSLFDAIHACRAEMLQVHAHMEDAGVSTALVFDLSSMDA
                     SLDRMQQLAAAFAADAKRDLQPARQLLNTLINARLEDTRILPLIRQNVNLLARKTVER
                     SGHSGEHYIANNLKEYWQMWLAAAGGGLLTVFTAAVKLRVMQAPHPLFVEGFLIGTDY
                     AISFLIMHTFRLALATKQPSMTAAAFAGIVRENRGVSRWSKIADFAARICRTQLAAAF
                     GNVLAVCLGAVVFEQLWLHIFSAPYLPQKTATYVYETLHPLTSGTAIYAAVTGVVLWL
                     AAVVGGWCENFAVYNRIPQAIAQHPLGMKIGTRRMQRLAHWFEDNISGWSTNITLGYM
                     LGFTPEIAHFFGIPLDVRHVTLSTGMMALAAARFGVSAFGHHWFYMAVAGLALTFFLN
                     LGVSFFIAANTGLRAYNVPHAERMQILKYVFGQAFRSPLRFLIPAETKEARTVEAQDR
                     SSS"
     gene            8437..9402
                     /locus_tag="VFA02_04615"
     CDS             8437..9402
                     /locus_tag="VFA02_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZU09163.1"
                     /translation="MVKVGVPHWTTKELLAKLPDEADIHILPEKPSAPIEIDFWIAPS
                     SLRHAESVAPYLRGVKVVQSLLAGVDWLKTLAPAGALLCDAQGAHNVATSEWTVAAIL
                     AWTKFFPFYFDLQRQQKWITRDIADANYHTVYGGDVSPYPPVLIEELEGKTVLIVGYG
                     SIGRSIEARLHPFGVNVLRIARTAKPGVEAVDKLAELLPQADIVVLIVPYTAETRGLM
                     NAAMMAKMKQGALLVNAARGPVIETDALVKALGERRIRAALDVTDPEPLPSDHPLWKA
                     PNLLLTPHIGGSSPLFMPRAFAVAAEQVRRFIKGEPLMNVVEGDY"
     gene            complement(9396..12317)
                     /locus_tag="VFA02_04620"
     CDS             complement(9396..12317)
                     /locus_tag="VFA02_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZU09164.1"
                     /translation="MAVTLTARLPVLNNAQHPHEGFSAAKELEQKLRSVVRGEVRFDP
                     GSRALYATDASNYRQVPIGLVIPRDVDDVIAAVSICREVGAPVLARGGGTSLAGQCCN
                     VAVVLDFSKYMRNIHWLDAEKKQAHVEPGIVLDRVREAAEEHHLTFAPDPATHNRCTL
                     GGMIGNNSCGVHALMGGKTVDNIHSLDVLLYDGTRMTVGPTSEDELSAIITAGGRKGE
                     IYAGLRRIRDQYADLIRQRFPRIPRRVSGYNLDELLPENGFNVARALVGTEGTCVIVL
                     GATLELMPSPPHRRLVGLGFADPFIAADYVPFVLEHKPIGLEGFDGMLVDFMRRKRLA
                     LDDIALLPEGRGHLLVEFGGWSPEDVEAQVRQFLEEVKQIPGQARRYSEPEAPRVWHV
                     RESALGSTVFVPGEPHGWEGWEDSAVPPEKLGAYLRDIFHLMDEFSYRTPMYGHFGQG
                     CVHMRINFDLESEEGIGKFRVFLDRATDVVLKYGGSISGEHGDGQARGALLPKMFGPE
                     LMQAFGEFKSLWDPENRMNPGKMIDPVAVYGPHENLRLGAGYKSVKPVTYFQFPEDKG
                     SFGEATLRCVGVGACRKQNPGTMCPSYMATQEEMHSTRGRAHLLWEALEGHVLEHGWG
                     DEHVREALDLCLSCKACKSECPVNVDIATYKAEFLAHYYEKHRHALRDYAFGFMDRWA
                     HLASAVPGVTTRLANLPLQVPGVSSLIKSILGIAPQRRLPCFAPRSYQSSLAQSLGNG
                     QVFLWPDTWNNYYHPQVLAAARQVLADAGFSAYTPRQHICCGRPLYDFGFLKHARAYL
                     ETVLSMFAREIDSGMPFIFLEPSCASVMRDELLNFFPTNERAKRLSEQALLLSEFLAQ
                     RAPEYRPPSLAGKRIVLHGHCHHKSLMKMKDEVAILKAAGADVELLDSGCCGMAGPFG
                     FEKEKYDVSQMLGERVLLPAVRHAAAETLIVSNGFSCREQIAQNSSRRALHFAEVLGS
                     Q"
     gene            12453..>12912
                     /locus_tag="VFA02_04625"
     CDS             12453..>12912
                     /locus_tag="VFA02_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZU09165.1"
                     /translation="MKTHRGFALKCGAAAMSALLALSGTPIQALAQSNDANTTTPIKH
                     VIVIIGENRTFDHVFATYEPKGHQKIWNLLSKGIIHKDGTPGPNYYLSAQYNAQDTDK
                     YSNSPQDKSLYFKLPPLMTGSAPTAASDTSPAPFKTLEVAELADTGLAPDY"
BASE COUNT         2646 a         3958 c         3558 g         2750 t
ORIGIN      
        1 aaagctcttc ttccaatgtc atcgaacggt ttttcttcgc gctcttcgcg gtcgcacggt
       61 ctgcgctcgc tgttcagcat gttttccagt gacctggcca ttgatctggg tactgccaat
      121 actctcgtct atgcccgcgg aaagggcatc gttgtgaacg agccttccat tgtcgccatc
      181 aacaaaaaca cgggcgaagt ggaggcggtg ggcaaagagg ccaaagagat gctgggccgc
      241 acgcccggaa acattgtcgc catcaagccc atgaaagatg gcgtgattgc cgacttcaag
      301 gtcacggaaa aaatgctgaa ctacttcatc cagaaggcgc acaaccgcaa gatgctggtg
      361 catccgcgca tcgtgattgg cgtgccctct gagattacgc aggtggaaaa gcgcgccgtc
      421 gaggactcgg cctatcgcgc caaggccagc gaagtgttcc tcgtggaaca ggccatggtg
      481 gccgccatcg gcgcgggcct gcccattaca gaaccatccg gcaacatggt ggtggacatc
      541 ggcggcggga ccacggacat cgcggtcatc tcgctggccg gcatcgtcta ctcgcggtcg
      601 gtccgcatgg cgggcaacca gatggacgag gccatcatga actacctcaa gcgcaagtac
      661 aatctgctga ttggcgaacg caccgccgag cagattaagc tggagatagg ctccgcatat
      721 ccgctggaaa agccgctgac gatggaaatc aagggccgca acctgattga aggcgtgccc
      781 aagaccatta ccattgatga tggcgagatc cgcgaggcac tgggcgagtg catcgccacc
      841 attatgaacg ccatccgcgt cgcactggag cggacgccac ctgagctttc ggctgatatc
      901 agcgaccgtg gcattgtgtt gaccggtggt ggcgctctgc tcaagaacct ggacaagcgc
      961 atccgcgagg agaccgggct gcccgtttcc atcgccgacg acccgttggc cagcgtggtt
     1021 ctgggtacag gaaaaatgct cagcgacttc cgtttgctgc gtaagatcaa aattgactag
     1081 ttgtgagtat cttgctggca ggtcaacaat aaatcaggca tcagaaccgt ggaatccttc
     1141 ttcagccgct ataagaacgc cctggtcctg atcctggtgc ttctggtgca ggtcatcggg
     1201 gtggccatcc aggtgcgccg tcctctcccc cgggagcacg cgcagcctga cgccggaggt
     1261 gtgcgcctga ttcgctactg ggccatcagc ctggtctcgc cgcccgagcg tctggtgcac
     1321 tggatcggca atgggatcag cggcctgtgg tccaattaca ttgacctcat ccatgtccgc
     1381 cagcagaaca aggatcttca ggccgagctg cggcggcttc gcattgagca gggaggcgtg
     1441 gccgaggacg cgcgccaggg ccagcgactt caacaactgc tggcgtttac ggaaaaatat
     1501 atctacaaga ccgttccggc gcagatcatc ggcacctccg gcctcgacca gtcccgcatt
     1561 ctctacatag acaaaggcgc caaagatggc gtggccgtcg acatgcccgt cattacgccg
     1621 gacggcatcg tgggccgcgt gcgcgaagtc tttccgcatt cggcacaggt gcttgagatc
     1681 agcgacccga ccagcggagc cggtgtcatc ctggccgata cgcgcattcg cggcattctg
     1741 cgcggaaatg cgctggggca gccggtcatc gtaggtataa tgccggacga gcgtatcaag
     1801 cccggggaga aggtcgtcac cagcggggga gaccagattt ttccccgcgg ccttgccgtt
     1861 ggcgtggtgg acaaagttgt tccggatccg gagcgcgacc cgatggtgga catcgtgatt
     1921 cgcccggcgg cagacctttc gcgtctggaa gaggtgcttg tcatcacgga catggggtcg
     1981 cagattaccc cgaaagaaaa gcttgacctg atgcaaagtc aggccattgc tgattcagag
     2041 caactgaagg catccgagat tctggccgag cgcctcccca gccgcattga tccggacaat
     2101 ccggaagcag caaaagacac tgatacggtt ggagcagatg gcgagattgc gcggcccatt
     2161 cagccgccgc cgccgttgca tcctgatcat ttctcgccca atgccacgcc acctgccgcc
     2221 agtctggttc cgggggcaag ggcagcagcc gtcttgcagg atacggcaaa caagacaacg
     2281 gccggcgtgc ccaagccgcg tccggccgtg ccagccaatg gcgcaggcag taccgctccg
     2341 gtaacggctt cgacggccac ggcggccccg cccaggcaga aatcccctga gctggttccg
     2401 gatgacggca gccgcccccc ggccaccgct gtgcagcgcg ctcctctggc cacgcagccc
     2461 actgccggaa atggtcaact caggcggagc gtatcggatt cgactcctgt tgcggccccc
     2521 tctgctttgc ctaagaccat cattgtcacg gatggtccca gaaagatgac acctccggcc
     2581 cctgccacgc agaaggcccc gggagcagca accaccactc cacaaaaacc cccggcgcag
     2641 caacacaaag ggccggagct ggtcccggat gacggcagcc gtccgccgga aagcatgctg
     2701 cacaagccag tgaagcccca gacccagccg cagacccaga ccgccccgca acaacccgca
     2761 ggaggaccgc agcgatgagt atgttgtccg ccgctgtgcg gcgcgattcg gagagtcagt
     2821 cgcatcctct gctggtctat gttcttgctc cgctgctggc gttggggttg cagtctttcg
     2881 ttgcgctcca ctttcacggc ttcgccattc tggacctgcc gctcctggtc acgatttatt
     2941 tcgccatctc gcaccggcat ccggtcgcag ggaccgttac aggcgcgctg attggaatcg
     3001 cgcaggatgc gctgacacgc catccgctcg gcatcttcgg aatctgcaaa tctattattg
     3061 gatatctggc ggcttcactc ggcgtgcgca tagataccga gaaccctgcc acccgggcgg
     3121 ccctgacctt ctgttttgtt tttctgcata gcggaattta ctggcttctg gtgcgccgtc
     3181 ttctggcgca gccctttctc tggatctggc tgcacgagct tctgcgcgcc gtagtcaatg
     3241 ccgttgtggc tgtggtgctc tttgccttgc tggacaagac gcggcgcaag gaatattagg
     3301 gggcctccga gcaaccggca gatcgctgaa aacaattgct ggggttgaaa tgaaagttcg
     3361 tttgtttttt gaaggagcag gaatagctat cctggccttc attgctgctg tctgggtctt
     3421 tctgttgcca gaccaccttg tgatttatca caacccgcgg cctctttcta caattgcagg
     3481 cgcgctggcc ctggatcttt tcttcgtaac cattctgttc ttcgctgtat tcatctttct
     3541 ggaccatctt ggccctgcca aaacaaggat tgcatgggcg gccattgctg catgggtgct
     3601 gacccgcctg gtgaacatca ccatcttttt tctgaactac tatgacgcgg aaaatgttcc
     3661 cggacgcaaa cagaaatctc tgcttcagtt tgcgatcttt ggcggagttt ttctgctctg
     3721 gtggctggga cgtgaaaaat ttcaggcatt cgttcgcgct gtaagaatgg gcctggcgct
     3781 gactggttgt tgtatcgtat ggatgcttcc gcaattgtct gtgcttgcgc tgcacactcc
     3841 cgcgaaagag atatctacat ttacgaatcc aaatatcacc gctgtcccgc catcttcaga
     3901 aaggattgtc tggatactct tcgatgagct ttcttatgac caggtattcg atcatcggca
     3961 gcccgatgtg cttttgccgc acttcgacca gctcagagat gaaagcgttg ccttcgctaa
     4021 cgtgcagcct gccggatact ttacggacat cattgttccg tcgctctttc tcgggcgcga
     4081 actgaagggc atccgaagct ctatcgaggg cgatctatat gtccgcccgg cccagaccaa
     4141 acactgggag ccattcaatc cctatgcgac tgtgttctac gacgcgcagc agtccggctg
     4201 gagcacaggt gtagcaggat ggtttaaccc ctactgtcgt attctttccg gcgttctcga
     4261 ttcgtgctat tgggaatcca tcaacccctt cgagcatggc ctctcagacc agaagtccac
     4321 gcttgccaat gcaatgagtt tcttcaaggg cgtgggcatc tcgcgtatca ccggtcctgc
     4381 atcggctgag cggtctgccg cggaggtcca cgcgcaggaa tacaacaatg ttctccctgc
     4441 cgcaacggct ttgatacgca atgatgcgat tcgctttgcc tttgtccatc ttccagtgcc
     4501 ccatcccccg ggaatgtatg acaggcatac gcataccttc agtaagcgcg gaacatatct
     4561 tgacaatctt gtccttgccg accaggcgct gggcacactt cttgacgctc tggccaggac
     4621 cgcagatgcg tcgcacacgg tcctgattgt ctcatcggac cactcatggc gtgtgggcat
     4681 gtggaagatg aacaatgtcg catggacaga tgaagaagag cacgccaccg gaggcaaatt
     4741 cgattcccgc cctgtcctgc tggtccattt ccccggcgaa aatcatggag aaaccaggca
     4801 gcgacccttt cctgaaatca gggaacgcgc attgatagac gccctattgc agggaaaact
     4861 gcgctcgcag agcgatctgg accagtggcc cgtcccaaaa taattgcagg cgcccgaaag
     4921 gcgcctgcat ctttcagact tgcgactccc ctccccccag tgaagccgca tgtctgaaac
     4981 cctggaaatt acatcggcaa tccgttatat gcatccacgt agggtttaag ctggtcgagc
     5041 acgacttcaa tcggcacggc ctgcccttgc tctaccttca ggcgcggatc attgacgatc
     5101 ggcaccagca ccaggcggtc ttccacccgc cggcctacaa agtacatgtt gaacgccggg
     5161 atgcgaacgg caaactcacc ttcattgtga cggtttccgg caagccgttt cacaatgtct
     5221 gcattgccaa agcctgaagg ctcatatccc tgttctttat ggacaatcat cacgcccgtc
     5281 ttcacctggc cgtctacagt cacgggatag atggtttcag ggctgggcgc cagcagcgta
     5341 ttcgggtcct gccctgcctg atatcccttc agcttgtcca ggccaatgtt gtagacctga
     5401 atcggcgtgg ccagctgcgc gcgctttact tcatccagag aatcgaaccc gaggcttttg
     5461 tagttttgct catttactag tttttgcagc acgccaatgg cgttttccgc cgcctgctgc
     5521 ggctgttgtg ccggaggtgg cggctgactc gggtgtctgg agcaacctgt cacaagaaaa
     5581 acacccaggg caagaattcc gagggaccgc atgttattgt cctcccgtat acgtgatgtt
     5641 gtaaaaatcg ttccagtgag tgtggtccca actcgctccg cctacatatt tgtcgtaggt
     5701 gtagacctcc tgcacaccac cggtgggagg ccagggattg ttcacgctca cataatttgt
     5761 tccgtcaatg gttacatatc cggtggccac catcatgtgc ccgccgcctc cgttccagtg
     5821 ccaggaaaag gcgaaaggct tcttcgcgca atatatctgg ctctggatct gcgcccacgt
     5881 caacggagca tcggaggtct ggtctgcctt aaagctgtat ttgtcatatt gcggccagcc
     5941 gccgtttaca cacgctgaag gcacaggaga gttgcagcag tcgctgcggc cgaactcctt
     6001 gtttgcctca tcgcactgct gcacgttcga gcctggatga atatagttca tggtcatctg
     6061 tccgctcgcc gcccagcacc acatattggt ttgctgcccg tttaaagtca ccggaactga
     6121 gccgatgttg cccggagtgc agcaccccgc caaaaaacca gacagtgcaa gaacacacac
     6181 tgcctgcatc gctttccaca tggaacgcct ccctcacgcc tggccctccc ctcatgtcag
     6241 gggccatttg cttgcagggt agtgccgggc aaggggccgg cgtttcaccc ggttgcagat
     6301 atttcctcga aacacgctac tatttgtgat gaaacgatgc ccctgttgag gacaacccgc
     6361 cggcttcttc gccagcgccg ctggaaaaag cgcgccgttt ttgcccagcc tgaccggaac
     6421 ctgaaggaac gcacacgagc attctgtgaa gcatccaccc tggcggcaca acagcggcac
     6481 attatcgcgc tgttctccta tatctggaac cggccgtcag accgcgggtt cgcctttgtt
     6541 ttgttgcgct ggatcaggct tctcgagcag gacaggccat tacgcgcgca gtttgagcgc
     6601 tcctggatta cgctgctttc cggcctggac gctgtttctg tcttttccga taccgggctt
     6661 cccgcgcacc atgcgcttct tccggaggcc ttccgccgta tctttcaacg gctcctgcct
     6721 tcggcacgcg aagagtcgga catggcccgt ctcttcacca cggtttttcc ttcgccgcgc
     6781 gccatcgggc gctttgttca gcttgaccgg acaacatttc accgcctggc ccgtgtcctc
     6841 tggcctaaag gcggccttga cgccttgccg cgtctccgcg acgatctgcg ccagtcgctg
     6901 attctgcttg ccacccgcat tgccggacgc ggcgcttctg ctcccatacg caaacggagc
     6961 agtatcgcaa gcgcggaaga atcgtctttt tataagcttg tcttcgccac agaagcattt
     7021 gtgcagaccg tagaagaaag tccacacaca gcgctgcatt ctttgttcga cgccattcat
     7081 gcgtgccgcg cagaaatgct tcaggtacat gcgcacatgg aggacgccgg ggtgagcacg
     7141 gcacttgtgt ttgacctgag cagcatggac gcctcgctcg accgcatgca gcaacttgcc
     7201 gcggcctttg cagcggatgc gaagagggac cttcaacctg cgcgccagtt gctgaatacg
     7261 ctgataaacg cacgcctgga agacacccgc attcttccgc tcatccgcca gaacgtaaac
     7321 ctgctggcgc gcaaaaccgt cgagcgcagc ggacacagcg gcgaacacta catcgcaaat
     7381 aaccttaaag agtactggca gatgtggctg gccgcagccg gaggcggcct tctcacggtc
     7441 tttaccgcgg ccgtgaagct gcgcgtgatg caggccccgc atcctctgtt tgttgaaggc
     7501 tttctgattg gcacagacta cgccatcagc tttctcataa tgcatacctt ccgcctggcg
     7561 cttgccacca agcagccatc catgaccgcc gcggcctttg ctggaattgt gcgtgaaaac
     7621 cgcggcgttt cccgctggag caagattgcc gattttgccg cgcgcatctg ccgcacgcag
     7681 cttgcggcag ctttcggaaa cgtgctcgcc gtctgcctgg gcgcggttgt cttcgagcag
     7741 ctctggctgc acatcttctc cgcgccgtat ctgccgcaaa agaccgcaac ctacgtgtat
     7801 gaaaccctgc atccgctgac ctcgggcacg gccatctatg ccgcagtcac cggagtggtt
     7861 ctgtggctcg ccgctgtggt gggcggatgg tgcgaaaact tcgccgtcta caaccgcatt
     7921 ccgcaggcca ttgcgcagca tcctctggga atgaagattg gcacgcgccg tatgcagcgc
     7981 ctcgcccact ggtttgagga caacatctcc ggatggagca ccaacattac gcttggttac
     8041 atgcttggtt tcacgcccga gatcgcgcac ttcttcggca ttccgctcga tgtccgccac
     8101 gtgaccctga gcacaggaat gatggccctc gccgcggccc gcttcggcgt cagcgccttc
     8161 ggccaccatt ggttttatat ggctgtggcc ggactggctc tgacgttctt tctcaatctg
     8221 ggtgtcagct tcttcattgc cgccaatacc ggcctgcgtg cctacaacgt tcctcatgca
     8281 gaacgcatgc agatactcaa atatgtgttt ggacaggcct tccggtcgcc gctgcgcttc
     8341 ctgattccgg cagaaacaaa agaagcccgg acggttgaag cacaggaccg ttcctcttcc
     8401 tgaattagca tcaggagagg aggacatcat ctgcgcatgg taaaagttgg ggtccctcac
     8461 tggacaacaa aagagcttct ggcaaagttg ccggatgaag cagacattca cattctgccg
     8521 gagaagcctt cggcccccat cgagattgat ttctggattg ctccttcctc gctgcggcat
     8581 gccgaatccg tagcgcctta tctgcgcggc gtgaaggtgg tccagtcatt gctggccggg
     8641 gtggactggc tgaaaacgct ggccccggca ggcgcgctgc tttgcgatgc gcagggcgca
     8701 cacaatgtcg ccacatcgga atggacagtg gctgccattc ttgcctggac aaagtttttc
     8761 cccttctact ttgaccttca gcggcagcag aaatggatca cgcgcgacat tgcagatgcc
     8821 aattaccaca cagtctatgg cggagatgta agcccgtatc ctcctgtact gatcgaggag
     8881 ctggaaggca aaacggtgct gattgtgggc tatggctcga ttggcaggtc tattgaagcg
     8941 cgattgcatc cgtttggcgt gaatgttctt cgcattgcgc gcacggcaaa acccggagtc
     9001 gaagcagtgg acaaacttgc agagctgctt ccgcaggccg acatcgttgt cctcattgtg
     9061 ccctacaccg ctgaaacgcg cggcctgatg aatgcagcga tgatggcaaa gatgaagcag
     9121 ggagcgctgc tggtgaatgc ggcgcgcggc cccgtcatcg agacagatgc gcttgtcaaa
     9181 gcgctgggag aaaggcgcat ccgcgcagcc ctggacgtca ccgatccgga gccattgcct
     9241 tcagaccatc cgctatggaa agcgcccaac ctgctgctga ctccgcacat tggcggctca
     9301 agtccgcttt ttatgccaag ggcctttgca gtggcagcag aacaggtccg ccggtttatc
     9361 aaaggcgaac ctctgatgaa cgtcgtggaa ggcgattatt gactcccgag gacctcggca
     9421 aaatgcaacg cgcggcggct ggagttctga gcaatctgct cgcggcaact gaaaccattg
     9481 ctgacgatca gcgtttcagc cgcggcatgg cgcaccgcag gcagcaatac gcgctcgccc
     9541 agcatctgtg acacgtcata tttttccttc tcgaagccga acggcccagc cataccgcag
     9601 catccggaat cgagcagttc aacatccgcc ccagccgctt tcaggatggc cacttcgtcc
     9661 ttcatcttca tcaacgattt gtgatgacag tggccatgca gcacgatgcg ctttccagca
     9721 agcgatggcg gccgatactc tggcgcgcgc tgtgcgagaa attcgctgag caagagcgcc
     9781 tgttcgctca atcgctttgc gcgctcattc gtcggaaaga aattcagcag ttcatcgcgc
     9841 atgacgctcg cgcagctcgg ctcaagaaag atgaatggca tgccggaatc aatctcgcgg
     9901 gcaaacatgc taagcaccgt ctcaagatat gcgcgggcat gcttcagaaa gccaaagtca
     9961 tagagcggac gtccgcagca gatgtgctgc cttggcgtgt atgcggaaaa tccggcatcg
    10021 gccagcacct gccgcgcggc tgcaagcacc tgcggatggt agtagttgtt ccaggtatcg
    10081 ggccagagga acacttgacc attcccgaga ctctgcgcga ggctgctttg ataactgcgc
    10141 ggcgcaaaac atggaagcct gcgctgcggc gcaattccga gaatgctttt tatcagcgag
    10201 ctgacaccgg gcacttgcag aggcagattc gccagccgcg tcgtcacacc gggcaccgca
    10261 gaagccagat gcgcccagcg gtccatgaag ccaaatgcat agtcgcgcaa cgcatggcga
    10321 tgtttctcgt agtagtgcgc aagaaactcc gccttatacg tggcgatgtc cacgttcacg
    10381 ggacattcgc tcttgcacgc cttgcaggaa aggcacaggt ccaatgcttc ccgcacatgc
    10441 tcatcgcccc atccgtgctc aaggacatgc ccttcgagcg cctcccacaa aagatgcgcg
    10501 cgtccgcgcg tggagtgcat ttcctcctgc gtggccatat agctggggca catcgttccc
    10561 gggttctgct tgcggcatgc gcccacgcct acgcagcgca gcgtggcctc tccgaaagag
    10621 cctttgtctt ccggaaactg aaaatatgtc actggcttaa cagacttgta tcctgctccc
    10681 agacgcagat tctcatgcgg tccatacacc gcgaccgggt ctatcatctt gcccggattc
    10741 atcctgttct ccgggtccca cagcgatttg aactcgccga aggcctgcat cagctcaggc
    10801 ccgaacatct ttggcaggag agcgccgcgc gcttgtccat cgccatgttc gcccgagatc
    10861 gaaccgccgt acttcaggac cacatcggtc gcgcggtcga gaaatacgcg aaatttgccg
    10921 atgccttctt cgctctcaag atcaaagttg atgcgcatat gcacgcatcc ctgcccgaaa
    10981 tgcccataca tcggcgtgcg gtagctgaac tcgtccatga gatgaaagat gtcgcgcaga
    11041 tatgcgccca gcttctccgg aggaacagcc gagtcctccc atccttccca tccatgcggc
    11101 tcgccgggaa cgaaaaccgt tgatcccagc gccgattcgc gcacatgcca cacgcgaggc
    11161 gcttccggtt ccgaataacg gcgcgcctgg ccgggaatct gcttcacttc ttcaagaaat
    11221 tgccttacct gcgcctccac atcttcaggc gaccagccac caaactcgac cagcagatgc
    11281 ccgcgccctt ccggcaacag cgcgatgtca tcgagcgcca gccgtttgcg acgcataaag
    11341 tccaccagca ttccatcgaa gccttcaagt ccaataggct tgtgctccag cacgaaaggc
    11401 acataatcag cagcgatgaa tggatcagcg aagccgagac ccaccaggcg gcggtgcgga
    11461 gggctgggca tcagctccag tgtcgctcca agaacaatga cgcaagtgcc ttctgttccc
    11521 accagcgcgc gtgccacgtt gaatccgttt tcaggcagaa gttcatcgag gttgtagccc
    11581 gagacgcggc gcgggatgcg cggaaaccgc tggcgaatca aatcagcata ctggtcgcgg
    11641 atgcggcgca gtcccgcata gatttcgcct ttgcgcccgc ctgcagtgat gatggcactc
    11701 agttcgtcct cgctggtggg gccgacggtc atgcgtgtgc cgtcatacag aagcacgtcg
    11761 agcgagtgaa tattgtctac tgtcttgccg cccatcagcg catgcacgcc acaggagttg
    11821 ttcccaatca tgccgcccag tgtgcagcgg ttgtgtgtcg ccggatcagg cgcgaaggtc
    11881 aggtggtgct cttcggcggc ttcacgcaca cggtcgagca caatgcccgg ttccacgtgc
    11941 gcctgcttct tttccgcatc gagccagtga atgttgcgca tgtactttga gaaatcgagt
    12001 accacggcga cattgcagca ctgcccggcc aggctcgtgc ctccgccgcg cgccagcacc
    12061 ggagcgccca cttcgcggca gatcgagaca gcggcaatca cgtcgtccac atcgcgcgga
    12121 atgacgaggc caatcggcac ctggcggtag ttggaggcgt ccgtcgcata cagcgcgcgc
    12181 gagccggggt cgaaacgaac ttccccgcgg acgacagagc gcaatttctg ctccagctcc
    12241 tttgcggcgc tgaaaccttc gtgaggatgc tgcgcattgt tcaggacagg gagccgggcc
    12301 gtaagagtca cagccatatt ttaggcgcga gtctggttga aaccaatttc tagcaaaaca
    12361 gtaatgtccc attcatctgc ggcgtgtact cctgtggtgt cgcgcacctg ctctgcgtgg
    12421 caccctattg ctccatcccc aggaggactc tcatgaaaac ccacagaggc ttcgccctca
    12481 agtgcggcgc agcggccatg tcggccctgc tggcgctttc cggcacgccg attcaggcgt
    12541 tggcccaaag caacgatgcc aacaccacaa cacccattaa gcatgtcatc gtcatcattg
    12601 gcgaaaaccg caccttcgac catgttttcg ccacgtatga acccaaagga catcaaaaga
    12661 tctggaacct gctctcaaag ggaatcatcc ataaagacgg cacaccaggt ccaaactatt
    12721 atctttccgc ccagtacaac gctcaggaca ccgataagta ctccaacagc ccacaggaca
    12781 agagtcttta tttcaagctt cctcctctga tgaccggcag cgcgcccact gcggccagcg
    12841 atacttcccc agcgccattt aagaccctgg aagttgcaga gctggccgac accggactcg
    12901 ctccggacta ca
//
LOCUS       DASRBV010000117         8151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_106879, whole genome shotgun sequence.
ACCESSION   DASRBV010000117 DASRBV010000000
VERSION     DASRBV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106879"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..743)
                     /locus_tag="VFA02_04630"
     CDS             complement(9..743)
                     /locus_tag="VFA02_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZU09166.1"
                     /translation="MAEHAVVSRQEWLDARLALMAKEKALTHQYDALVRERQQLPWVR
                     VEKKYVFDSPEGKKRLADLFEDKSQLFIYHFMFGPEWEQGCPSCSLLMDSVNGNYIHL
                     TQRDVSLVLVSRAPMEKIAPFKKRMGWSVPWVSSYGNDFNRDYAVSFSHDEMNGNNYN
                     FGTLRPYADESPGVSAFYKDMDGSIYHTYSSYARGPENLMSIYAILDMSPKGRNEEGL
                     PWPMAWVRHRDRYETAKPAAACCTHE"
     gene            complement(777..1580)
                     /locus_tag="VFA02_04635"
     CDS             complement(777..1580)
                     /locus_tag="VFA02_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZU09167.1"
                     /translation="MATNKGTEKEHLTMNTPPVVSPQEWEAAWQQLLVKEKALTRAKD
                     ALAAERRRMPWMAVQKKYEFDGPDGKVSFLDLFEGRRQLIVYRAFFEPGVFGWPDHAC
                     RGCSLGADQVGHLSHLNARDTTLAWVSRAPQPDIARLKERMGWKMPWYTITDSFDADF
                     GVDQWHGHNVFLRDGDKVFRTYFINNRGDEALGPVWSYLDITPLGRQETWEDSPEGYP
                     QTPPYQWWRWHDDYGTETATDPRWLDIISDPYGAALRRQEAEKKRCGCS"
     gene            complement(1603..2064)
                     /locus_tag="VFA02_04640"
     CDS             complement(1603..2064)
                     /locus_tag="VFA02_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZU09168.1"
                     /translation="MLVTVSEQTLHTLEFLREEEIAAPVDVVFETVLEYMGPLNEAPG
                     SPMPMKLEAWPGGRWFRDLGDGAGHWWGTVQAIKPPTMLEICGPMFMSYPAISNVQYR
                     LKEENGVTLLKFVHRAMGYIAYDPQIEEHWRGVETGWGNILDKIRTASVKK"
     gene            complement(2088..2450)
                     /locus_tag="VFA02_04645"
     CDS             complement(2088..2450)
                     /locus_tag="VFA02_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU09169.1"
                     /translation="MARAATITDVFNAVAEPRRREIIGLLSDGREWTVNDVVARLRLG
                     QPTVSKHLGVLRKVGVVIVVRRGQHRMYRLEAANLKPIHDWASTFERFWNQKIDHIKE
                     RAEQKAMERIAKENLKLK"
     gene            2657..3706
                     /locus_tag="VFA02_04650"
     CDS             2657..3706
                     /locus_tag="VFA02_04650"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HZU09170.1"
                     /translation="MSQSAYQFPHRRYNPLRRQWVLISPHRTQRPWQGEVNQSSGFSD
                     VHYDPACYLCPGNERAGGHCNPRYENVFIFDNDYAALLPDSPAPEVEGASELLRAEQE
                     RGRCRVLCFHPNHSLTLALMEEADIRRVVDSWTGQYEELAANPAIRYVQIFENRGAMM
                     GASNPHPHCQIWATEHVPDEPATETESLKAYQASHGSCLLCDYLKIETEKQTRIVCEN
                     DEFLVVVPWWAVWPFETLVLAKKHVGSLAEFTGAQKAALANILKQTTTRYDNLFQTSF
                     PYTMGFHQTPCDGEKHGEWHFHAHYYPPLLRSATVRKFMVGFEMLGMPQRDITPESAA
                     ERLRVLSGVHFTQSR"
     gene            3759..4535
                     /locus_tag="VFA02_04655"
     CDS             3759..4535
                     /locus_tag="VFA02_04655"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZU09171.1"
                     /translation="MNVDLEWIPAMGAKHLALCALVFASLTCPAFGEDPKSSTADPKS
                     KITDKKLRDFADRNRNIYYKNKLEYSLESGWLPYNIPFVFDFLVGSKYTEWPLHYTLV
                     PNITSVRWHIDNIWGWGPLRGNTDFTFSGAYTAVVRGPESRYFAFDYGIRRNFIQPNW
                     RVVPYFEARGGVGDINAKGPYGILYAQGQDLTFTLMMGTGARYNFNSKYSFSAGMTYM
                     HVSNFYLSEPKYEDFGINVYGPIVGINVRIGKPKERPEQP"
     gene            complement(4582..4657)
                     /locus_tag="VFA02_04660"
     tRNA            complement(4582..4657)
                     /locus_tag="VFA02_04660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4622..4624),aa:Lys,seq:ttt)
     gene            complement(4898..5593)
                     /locus_tag="VFA02_04665"
     CDS             complement(4898..5593)
                     /locus_tag="VFA02_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU09172.1"
                     /translation="MNVFQLHEQLRRELLRRIQHGTLTGTLLARQTGLKPAHISNFLH
                     GKRRLSLEALDRILAAQDLQIENLLPQIRSRDVSSTAECIPLVAQSTAMHEAKLRSSS
                     ILEQIQLPSGFLQHLTARRSPDRRAWDRFAAIRVDYSQAEPMMPVIRPHTVLVLDRHY
                     TSLTPYRPPLPNIYAIHTLSKLIIRYAELHGSQIVLRPEKLEHPIQLLDLKPKDLPSR
                     FIIGRVCGALFEL"
     gene            6231..6662
                     /locus_tag="VFA02_04670"
     CDS             6231..6662
                     /locus_tag="VFA02_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09173.1"
                     /translation="MSAALALPYIWAEAEAVEPRPHDGIAFYRKQTEQLLRRYVLVSL
                     QTGRLPSLIGNSVFRGKVSSYRMHSFEDAVIFVFDIEKCLKKLNSFEQELITRIALQE
                     YTYEETAELMQQCLKTVIRKYRDTLDKLSRIFLQHEVIDRL"
     gene            7042..7284
                     /locus_tag="VFA02_04675"
     CDS             7042..7284
                     /locus_tag="VFA02_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09174.1"
                     /translation="MDSLVGKSIDGSIQHTRLLFDLGGVKEELEESSKERKLGPSLGR
                     LLLKDAAEFRKRKEAEKAAKLVAAAADSSRPVKSLN"
     gene            7490..>8151
                     /locus_tag="VFA02_04680"
     CDS             7490..>8151
                     /locus_tag="VFA02_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09175.1"
                     /translation="MRVIATIAAVAMICAGHADAQVSTTTVQGVVYRADGTPATGTLI
                     VSWPAFSTTNNQAIAAGSTTAAIGQDGFVSVNLAANAGASPAGTYYTAVYHLSDGTVN
                     KEYWVVPQAASASLTSVRAQLVPATVAMQSATKQYVDASIASLSGNYLQLSGGAMSGP
                     LTLAGSPTAGNQAASKQYVDQAVQTAVPVSGGNMTGPLRLPTMISKLPRVDVRHPDFV
                     GGA"
BASE COUNT         1905 a         2115 c         2240 g         1891 t
ORIGIN      
        1 acattgcatt actcatgggt gcagcatgcg gctgcgggtt ttgccgtttc gtaacgatcg
       61 cggtgtctta cccacgccat gggccacggc aggccctctt cattacggcc ctttggtgac
      121 atatcgagga ttgcatagat actcataagg ttttccgggc ctcgcgcata actcgagtat
      181 gtgtgataaa tactcccgtc catgtctttg taaaatgcac tgacccctgg cgactcgtct
      241 gcgtaagggc ggagtgtgcc gaagttatag ttgttgccgt tcatctcgtc atgactgaat
      301 gacactgcgt agtcacgatt gaagtcgttc ccataagatg acacccaggg cacagaccat
      361 cccattctct ttttgaaggg agcgatcttt tccatgggcg cgcgtgagac caggacgagc
      421 gagacatcac gctgcgtaag atggatatag ttgccattca cggagtccat cagcagcgaa
      481 caggatgggc agccctgctc ccattccggg ccaaacatga agtgataaat aaacaactgg
      541 ctcttgtctt caaacaggtc tgccagcctt tttttccctt ccgggctgtc gaaaacgtac
      601 ttcttctcaa cgcgtaccca aggaagctgc tggcgttcgc gcaccaacgc atcgtactga
      661 tgcgtgagcg ccttctcttt ggccatgagc gcaagccgcg cgtccagcca ctcctgccgc
      721 gatactactg cgtgttctgc cattgccatc tcctcgttac attgaaattg cggggtttaa
      781 gagcaaccac atcttttctt ctccgcttcc tgtctgcgga gagcagctcc atagggatcg
      841 ctgataatgt ccagccatcg tgggtcggtg gccgtttccg tgccgtaatc gtcgtgccat
      901 ctccaccact ggtagggtgg tgtctgagga taaccttccg gggagtcttc ccatgtctcc
      961 tgacggccta gtggtgtaat gtcgagataa ctccataccg ggcctagtgc ttcatcgccg
     1021 cgattattga tgaaatatgt gcggaacact ttgtcgccat cacgcaagaa gacattgtgg
     1081 ccgtgccact ggtctacgcc aaaatccgca tcaaagctgt cggtgatggt gtaccagggc
     1141 atcttccagc ccatccgctc cttcagccgc gcgatatcag gctgcggtgc tcgcgatacc
     1201 caggcaagcg tggtgtcgcg tgcattcaga tgcgacaagt gaccaacctg atctgcaccc
     1261 agagaacagc cgcggcacgc atgatccggc catccgaaga cgccgggctc aaaaaatgcg
     1321 cgatagacga ttaactgccg ccgcccttca aacaaatcaa gaaagctgac tttaccgtca
     1381 ggtccatcga actcgtattt cttctgaacc gccatccacg gcatccgtct gcgttcagca
     1441 gcgagcgcgt ctttggcacg ggtcaaggcc ttctctttta ccagaagctg ttgccacgcg
     1501 gcctcccact cctgcggtga cacgacagga ggcgtattca tagtaagatg ctctttctct
     1561 gtccctttgt tcgttgccat atctgttctc ctgatgcgcg tgttactttt tcacagatgc
     1621 tgtccggatc ttgtcgagaa tgttgcccca tcccgtctct accccacgcc agtgctcctc
     1681 gatttgcgga tcgtatgcga tataacccat ggcgcggtga acgaacttca gcaacgttac
     1741 tccgttttct tccttcagcc ggtattgcac atttgagatt gccggatacg acataaacat
     1801 cggtccgcag atttccagca tcgtcggtgg tttgatggcc tgcaccgttc cccaccaatg
     1861 cccggcccca tcgcccaggt cgcggaacca gcgcccgccc ggccaggctt ccagcttcat
     1921 cggcatcggt gaacctggtg cctcattgag cggccccatg tactccagta ccgtctcgaa
     1981 aacgacatca accggcgcgg caatttcctc ttcgcggagg aactccaagg tatgcaaagt
     2041 ctgttctgat actgttacca gcattgcaat ctgttctctc ctcatttcta tttcaatttc
     2101 aggttttctt ttgcgattct ttccatcgcc ttctgttcgg cgcgttcctt gatgtggtct
     2161 atcttctggt tccagaagcg ctcaaaggta ctggcccaat cgtgaatagg cttcaggttt
     2221 gcggcttcca gcctgtacat gcggtgctgt ccgcgtcgca ccacgatcac cacgccgacc
     2281 ttgcgcagca cgcccagatg ctttgagacc gttggctgcc ccagacgtag ccgggctacg
     2341 acatcgttta cggtccactc tcgcccatcg ctgagcaagc cgatgatttc gcgccgccgc
     2401 ggctctgcca cggcattgaa gacatcggtt attgttgcgg cccgtgccat tggtctacag
     2461 atatattcct atataggaat atatgtcaag ccatgccatt gggattcaat ttatgtatct
     2521 gtatccgtag agatagcaat gcagcctgaa acgcgagagt cggcctccgg ctcgaggaga
     2581 cagccaacac actcagcaat agccctgcag tgctttatcg tagaagaagc ccatccatct
     2641 gaaaggaaag accccagtga gccagtccgc ctaccagttt ccccatcgcc ggtataatcc
     2701 gctgcgccgc cagtgggtgc tgatttctcc gcaccgcacg cagcgcccgt ggcaggggga
     2761 agtgaaccag tcttcaggat tttctgatgt gcattatgat ccagcgtgct acctgtgtcc
     2821 gggaaatgag cgcgcagggg ggcattgtaa tcctcgttat gaaaacgtgt tcattttcga
     2881 caacgactat gccgcccttc tgccagactc gcctgctccc gaggtggaag gcgcgtcaga
     2941 gctgctacgc gccgagcaag agcgcgggcg ctgccgtgtg ctctgcttcc atcccaacca
     3001 cagcctgacg ctggcactca tggaagaggc cgacattcgc cgcgtggtgg attcctggac
     3061 cgggcaatat gaagagctgg cggccaatcc ggcgattcgc tatgtacaga tatttgagaa
     3121 ccgtggcgcg atgatgggcg ccagcaatcc gcatccgcac tgccagatct gggccacgga
     3181 gcacgttccc gatgaacctg ctacggagac ggaatcgctc aaggcatatc aggcaagcca
     3241 cggctcctgc ctgctgtgcg attatctgaa gattgaaaca gaaaagcaga cacgcatcgt
     3301 ctgtgaaaat gatgaatttc tggtcgtagt gccgtggtgg gcggtgtggc cctttgagac
     3361 cctggtgctg gcgaagaagc acgtcggctc tctggcagaa tttaccggtg cgcagaaagc
     3421 agcactggcc aacattctga agcagacgac cacccgctat gacaacctct tccagacaag
     3481 cttcccgtac acgatgggat ttcatcaaac tccgtgtgac ggggaaaagc atggcgaatg
     3541 gcatttccac gcgcattatt acccgccgct gctgcggtca gcgaccgtgc gcaagtttat
     3601 ggtaggattc gagatgcttg gaatgccgca gcgcgacatt acgccggaaa gcgcggcgga
     3661 gcggctgcgg gttttgtccg gggtccactt tacccagagc cgttgaagag cggccagtcg
     3721 cattatgggc cgccaaaaaa gttgccgggt aagctgcttt gaacgttgat ctggagtgga
     3781 taccagcaat gggtgcgaag catctggcgt tgtgcgcgct cgtgtttgcg tctctgactt
     3841 gcccagcgtt cggcgaagac ccgaagagca gtaccgcgga ccccaaaagc aaaatcactg
     3901 acaaaaagct ccgggacttc gccgaccgca accgcaatat ctactacaaa aacaagctcg
     3961 agtactcgct cgaatcggga tggctcccct ataacattcc gtttgtcttt gatttcctgg
     4021 taggcagcaa atacacggag tggccgctgc actatacgct cgtgcccaac atcacgtcgg
     4081 tgcgttggca cattgacaac atctggggat ggggccctct gcgcggcaac accgacttta
     4141 ccttcagcgg agcttacacc gcggtggtcc gtggcccgga atcacgctat ttcgccttcg
     4201 attacggcat ccggcgcaac ttcatccaac ccaactggcg cgtagtgcct tactttgagg
     4261 cgcgcggcgg cgtgggagac atcaacgcca aaggaccata tggcattcta tatgcgcagg
     4321 ggcaggacct gactttcaca ctcatgatgg gcaccggcgc gcgctacaac ttcaattcca
     4381 aatacagttt ttccgccgga atgacctata tgcacgtgtc gaatttttat ctctcggaac
     4441 cgaagtatga ggacttcgga attaatgttt acggtccgat tgtggggatc aatgtgcgta
     4501 tcggaaaacc gaaagaacgc ccggaacaac catagacagc gaccgcgtta gatttacacc
     4561 ataaaaataa atgggatttt atggtgagcc cgctgggatt cgaacccagg acccacgcct
     4621 taaaagggcg ttgctctacc agctgagcta cgagctcacg cagactgcta cttcttccaa
     4681 ggtaacatag attcgccata ttcacagatg gcgctgagac ccacgatgtg ctttaaggcc
     4741 ttccttttca ctgcattctg ttcagtttga cgaactcttc cggttgcgca aacaacgcgc
     4801 gaaaggatga gacacccgat ggagtgacaa tcaaaacgtc gcaagataaa aattcgcact
     4861 tatgctgtgc gctccaaaag caaccgggct ttgttcttta tagctcgaag agtgcaccgc
     4921 agacgcggcc aatgatgaat ctggagggga ggtctttggg tttcaggtca aggagttgaa
     4981 tggggtgctc gagcttttcg ggacggagga cgatttgact gccgtgaagc tctgcgtagc
     5041 gaatgataag tttcgatagc gtgtgtatag cgtagatgtt tggcagcggc gggcggtagg
     5101 gagtgagtga agtgtagtgg cggtcgagaa cgagcacggt gtgtgggcga atgaccggca
     5161 tcataggttc ggcctgagaa tagtccacgc gaatggcagc aaacctgtcc catgcgcgcc
     5221 ggtctggcga gcggcgtgcc gtgaggtgtt ggaggaaacc agaaggtaac tgaatctgtt
     5281 ccaaaatgga actggaacgg agtttggctt cgtgcatggc ggtagattgg gcgacaaggg
     5341 ggatgcattc tgccgtggat gacacatcac gactacgaat ctgcggcagg agattttcaa
     5401 tctgcaagtc ttgcgcggcg agaatgcggt cgagggcttc gaggctaagg cggcgttttc
     5461 catgcagaaa atttgaaata tgcgcaggtt tgaggcccgt ctgtcgcgcg agcagtgttc
     5521 cggtcagagt gccgtgctgg atgcggcgca gcaattcccg gcgaagctgt tcgtgcaact
     5581 gaaagacatt cattgtggaa atttttctta gagctataca acgtgagagg gtatgtttgc
     5641 tcgaagaaaa gtaattctga tgcgtttccc tttatccatg ggggacattc ctgctgaggt
     5701 tgcacatatt ctctgtttgc gtggccgtgt ggttgacctg catttactcc gcagataaat
     5761 tctttttcac tttcgcctga atacatctga aattttgcct tcacagggaa tgctctctgc
     5821 aagtggagcc atgttttcct gtgggccggc gctattttgg gtctggtctt caggggaaga
     5881 gtgtttggaa gtttgtgccc caatgatctg catttctctt ttttatcttt cagcggagag
     5941 ctgcggcgca acagaatgcc gcgttcgatg ctgttccatt ctctgccttg ctctggcgaa
     6001 ccagatagca agtaatccag gtactggaga actcacacga tgtatatgct gcttaacctg
     6061 aaagtctgtg agggatgcgg gggcctctgg ctgcgcgtcc atgaatttaa caatgtttat
     6121 tgcgcaagtt gcgcggagaa actgcgggca tttccgagga caaaaaagca tccgcacaag
     6181 aagcatccgc ggcggcggcc acacacggca gatgcgaagg gaggagcacg atgagcgcgg
     6241 cactcgcact cccttatatc tgggcagaag cagaagcggt agaaccgagg ccacatgacg
     6301 gcatcgcctt ctacaggaaa caaacagagc aattgttgcg acgatatgtt cttgtatcgc
     6361 tgcaaacggg gcgccttccc tcactgatag gaaacagcgt gtttcgcgga aaagtatcga
     6421 gctaccggat gcacagcttt gaggacgcag tgattttcgt attcgatata gagaaatgct
     6481 tgaagaagct caatagcttt gagcaggaac tgatcaccag aattgctctg caggagtata
     6541 cctatgaaga aacagcagaa ttgatgcagc agtgcttaaa aacagtcata agaaaataca
     6601 gggacactct ggacaagcta tccagaatat ttctgcaaca tgaggtgata gacaggcttt
     6661 aaattgccgc ttactccatg atgcatggaa ttacgcatca tgggcctgcg atatcgaaag
     6721 atttgaaagg aaaaattggt gtcaagaggc ggggtatgtg gaaaactgga taacttatta
     6781 gatttaaagt agatagctta tgtaagaaag ttgtccagtt tactctgttc aacctgatat
     6841 tctgaactta agagtaaaga tttccgaggg gagcaagtta ttgcttccct tttttatttt
     6901 tccgacatgg taacggataa aactatcacg acacagcttg aaccggaagt tactgcgaag
     6961 gacattatca gaaaacggac tacggcaaaa caattcagga aaaaagccaa agcgcaaatc
     7021 gaaaagaact acgaggctat tttggattca ttagtcggta aatccattga tgggagtatt
     7081 cagcacacca ggctgctgtt cgatctgggt ggagtaaagg aagaactgga agagtcctcg
     7141 aaagagcgca agctggggcc gagtctgggg aggctgctgt taaaagatgc ggcggaattc
     7201 aggaagagga aagaagcaga aaaagcagcg aaactggtag cggcagcggc agattcaagc
     7261 cgtccggtca aatcactgaa ttgagagcag gagtgtcttt cacagtagca aggaattttt
     7321 cgttcagatg tacagtcccg tcttctgcta aaaagaacct ctcttcgttc catttgctgc
     7381 ggggcagcta caacaaaaaa ctttctccag aacagcccgt taaggcaggg cacagccgga
     7441 accttcggtg aacaggtgtg ctatgaaaac tttctttgct cagaaaacga tgcgagttat
     7501 agcaacaatc gcggcagtcg cgatgatctg cgctggacat gctgatgccc aggtaagcac
     7561 gacgactgtt cagggagtgg tgtaccgcgc ggatggaacg ccggcaacgg gaacgctgat
     7621 tgtgagctgg cctgcatttt caacgacaaa caaccaggcg attgcagccg gtagcacaac
     7681 cgcagcgatt ggccaggatg gattcgtgag tgtgaatctt gccgcgaatg ccggggcctc
     7741 tccggcaggg acctactaca cggcggtcta ccacctgagc gatggaacgg tgaacaaaga
     7801 gtactgggtt gtgccacagg cggcgagcgc gtcgctgaca tcggtgcggg cgcaactggt
     7861 tccagcaaca gttgcgatgc agagtgcgac gaagcagtat gtggatgcct cgatagcatc
     7921 gttgagcggg aattatctgc aactcagcgg cggggcgatg tccggaccgc tgacgctggc
     7981 gggtagcccc acggcgggaa atcaggcagc gtcgaagcag tacgtggacc aggcggtaca
     8041 gacagcggtg ccggtatctg gcggaaacat gacgggacca ttgcgcctgc cgaccatgat
     8101 ttccaagctg ccgagggtag atgtgcggca tcctgatttt gtgggagggg c
//
LOCUS       DASRBV010000118         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_56898,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000118 DASRBV010000000
VERSION     DASRBV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56898"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..840)
                     /locus_tag="VFA02_04685"
     CDS             complement(19..840)
                     /locus_tag="VFA02_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09176.1"
                     /translation="MNPAGPGFQTVNGTPFYLNGMYIAGQNGFPRGIVTNDYSTIQPR
                     VGFSWDMMGTGKTVLRGGFGTFYERMQGNDIYNTATNPPFANDPSASNVYLSNPHTSW
                     VSGQTASTPIFPQGLTTLARTYPAPAVAQFSLGVQHEVAPSVIWVVQYVGNLAVLLFH
                     PEEKPRLCVCMGRGLNHQHILSVFKRAEHGVFAGSPGSQVVRSVQVGKPLGLMTGKTG
                     CIPGIMIRMVRALLRCLDCTQGQKSGKRTDNHFHPRHGLAMQRQQLSLHWYAMIA"
     gene            complement(1327..3417)
                     /locus_tag="VFA02_04690"
     CDS             complement(1327..3417)
                     /locus_tag="VFA02_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU09177.1"
                     /translation="MSKAVKYAERAVTIAANGAWAVFERLNRISPNPSFTPKWSDKPL
                     LKSYEKEKPPLGWPRTTDSLCPKCVPEIRQQILDGKLPHEILLNEKVGEIKAQIIERD
                     GKILMVKDCPKHGHFEDIMSIDPEFSRHLEEVFPGRDIRAHNDEKLHNHGTSTITHGR
                     GSVLTIDLTNRCNMMCDPCFMDANQVGFVHELTWEEIKTMLDNAISIKPRRQMSVQFS
                     GGEPTLSPYFLDAVAYARKVGYNSVQAATNGIEFAKSKEFCKAAAEAGLRYAYLQFDG
                     IGNAANSHRKVGNLFDVKLQAIHNLHEAGVDIVPVTTIVNGINNEQVGRIIQFALDNP
                     KKINFLSFQPVSFTGRDEAVTDERRMAQRYTLSHMAHDVKNQTGLGEPARDWFPISFM
                     STFSDWADLVHGPNADWGQLSCGCHPNCGIGMALMIDKETKEAVPVTAFLNADRLAKD
                     VAKINDAARGKFLSIVGVALALLRNYDPRKSPTHFRITDLLQKFDKCFGATGRDYGKV
                     TGDRTMADIEKRRADRWNFLFIAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFCAYN
                     TGIGWRNIIEKMHMTATLTKWYEEHGRHEIFAGGKKVGMGNAEHTLVLNEEHVHAAAN
                     DTLDKLGIAKNAREEKIRARDAKLKQDAENARMAQLYRQHILGEKPAEGIVPITSIAP
                     AKPVPAAEEPVMGD"
     gene            complement(3730..4896)
                     /locus_tag="VFA02_04695"
     CDS             complement(3730..4896)
                     /locus_tag="VFA02_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09178.1"
                     /translation="MSLLGLSEYSPLPPLEPERPEKPKSAAIAFLLSVLLPGIGQVYA
                     NRRTAGWVTFTFFYLSLLMGIATLIAKQFNLSGIAIFIAISLYIFGFLDAYFSALEYN
                     QGISTYLIGGNPRIACFLNLLTNGFGYFYLGDRGKGIAMFLIVGVLGRSVKALSKSPG
                     FTWFDFILLLLQFAFALDGYRIARKRLFESFPQLANHSWRSSIAGHLTPAVPIAFAII
                     IILPVIALVSLGLFAQTATGINLSAMDVQSTSNGVAYENKLLGLRLLLPDGWQVTPQP
                     GNTNFNAEKVNGNCKVILIREFSFRSPESFQAKIEHNLSLRPGLSVYGHSSGTLGNLP
                     AAIVRVGVGTSFTEQVVSAKAGLAMYTLIGMQTGEDETCPAELDQIRNSFSTKH"
BASE COUNT         1158 a         1278 c         1422 g         1182 t
ORIGIN      
        1 gccgttccgg gaagaaattc acgctatcat tgcataccaa tggagagata actgctgtcg
       61 ctgcatagcg aggccgtgcc tgggatgaaa atgattgtca gtgcgcttgc cggatttctg
      121 gccctgggtg caatccaggc agcgcagcaa ggcccggacc atcctaatca ttatgccggg
      181 gatgcagcct gtctttcctg tcatcaggcc caggggcttt cctacctgca cactgcgcac
      241 cacctgactt cccggactgc cggcaaaaac tccgtgctcg gcccttttga agacggaaag
      301 aatgtgttga tgattcaggc cccggcccat gcagacgcac aacctgggct tttcttcagg
      361 atggaagaga agaacggcaa ggttgcctac gtactgcaca acccagatga cggaaggcgc
      421 cacttcatgc tgcacgccca ggctgaactg cgcaacagcc ggggcaggat aggtgcgagc
      481 gagcgtagtc agcccttgcg gaaagatggg tgttgacgct gtttgtccgg acacccagga
      541 agtatgcgga ttagaaagat aaacgttgct tgccgatgga tcattggcaa agggcggatt
      601 ggttgcagtg ttgtagatgt cgttcccctg catgcgctcg tagaaggtcc cgaagcctcc
      661 gcgaaggacc gtcttacccg tacccatcat gtcccatgag aagccgacac gtggctggat
      721 ggttgagtaa tcgttagtga caatacctcg tggaaaaccg ttctgtccgg caatgtacat
      781 accgttcaaa tagaacggtg ttccgttcac ggtctggaag ccaggtccag cggggttcat
      841 cgagccatca gagtttcaga gtggtgactg gctttgcaaa tattggccag gatcatcgag
      901 ccatctcatg gattgtcccc tcccgaagtt gcacgcctcc ccgctgtctg gcgtggaagg
      961 cgctggcagt tctaaaacga tttatgagct aggttgaact gcgggcagaa ctgtatgcga
     1021 gaagaaaaac aaatgtcaag aaattttctg gaccatcaga agagaaagtt catagcttgc
     1081 aatgacttgg taatgtatta ataaccagat agttaatttt ctagagaata gaatattcat
     1141 actctatatc tctatgtagc ttgcctatat tcattttcta tcaagagcaa tggctctgag
     1201 atcctgcttt tcactctgaa aatccggaag ctgaacgaca gaagaaaaag cctgatgaaa
     1261 caaaaaagtc cggcgcgagg ccggactttt tctttcaact gcattcagga caggctgcct
     1321 ggacagttag tcccccatga cgggttcttc ggctgcgggg accggctttg ccggagcaat
     1381 ggatgtgatg ggaacgatgc cctcggccgg cttttcgccc agaatgtgct gacgatagag
     1441 ctgggccata cgggcgttct cggcgtcctg tttcagcttg gcgtcgcggg cgcggatttt
     1501 ttcttcgcgc gcattctttg cgatccccag cttgtccaga gtgtcattgg cagcggcatg
     1561 cacgtgctcc tcgttcagaa cgagtgtatg ctcggcattc cccatgccca ccttcttgcc
     1621 gccggcgaag atttcgtgac ggccatgctc ctcgtaccac ttggtgaggg tggcggtcat
     1681 gtgcattttc tcaatgatgt tgcgccagcc aatgcccgtg ttgtacgcgc agaagctgat
     1741 ttcgccttcc tgtgtggcat acggaatgat gcactgctcg gtacggcgga agtcgtagtt
     1801 gaagaggtcc tggaaccaca ttccggcgat aaagaggaag ttccagcggt cagcgcggcg
     1861 cttctcgatg tcggccatgg tgcggtcgcc agtgaccttg ccgtaatcgc ggcccgttgc
     1921 gccaaagcac ttgtcgaact tctgcagaag gtcggtaatg cggaaatgcg taggcgactt
     1981 gcgcggatca tagttgcgca gcagcgccag tgccactccc acaatggaga ggaacttgcc
     2041 gcgggccgcg tcgttgatct tggccacgtc ctttgccaga cggtctgcgt tcaggaaggc
     2101 tgtgactggg acagcttcct tcgtttcctt atcaatcata agcgccatgc cgatgccgca
     2161 gttcggatgg catccgcagg aaagctggcc ccagtcggcg ttcggtccat gcacgaggtc
     2221 ggcccagtct gagaaggtgc tcataaagct gatggggaac cagtcacgcg caggctcgcc
     2281 cagtccggtc tggtttttca catcgtgcgc catgtgcgag agcgtgtaac gctgtgccat
     2341 acggcgctcg tcggtaacag cttcgtcgcg gccagtgaag gagaccggct ggaagctgag
     2401 gaaattgatc ttcttaggat tgtccagggc gaactggatg atgcggccca cctgctcatt
     2461 gttgatgccg ttgacgatgg tcgtaaccgg tacgatgtcc acgccggcct cgtgcaggtt
     2521 gtgaatggcc tggagtttca cgtcaaagag gttgcccacc ttgcggtgcg agttggcggc
     2581 gtttccaatg ccgtcgaact ggagataggc ataccgcaga ccggcttccg cagcggcttt
     2641 gcagaactct ttggatttgg caaactcaat gccgttcgtc gccgcctgca cggagttgta
     2701 tcccaccttg cgggcataag cgaccgcatc caggaagtac ggcgaaagcg tcggctcgcc
     2761 acccgagaac tgaaccgaca tctggcggcg cggtttgatg gagatcgcgt tgtccagcat
     2821 ggtcttgatc tcttcccacg tcagctcatg gacgaagccg acctggttgg cgtccatgaa
     2881 gcaggggtcg cacatcatgt tgcagcggtt ggtcaggtca atcgtcagga ccgagccgcg
     2941 gccgtgggtg atggtcgaag taccgtggtt gtgcagcttt tcgtcgttgt gggcgcggat
     3001 gtcgcggcca ggaaatacct cttcgaggtg gcgcgagaac tcggggtcaa tagacatgat
     3061 gtcttcaaag tgcccgtgtt tggggcagtc tttgaccatc agaatcttgc cgtcgcgctc
     3121 aatgatctgg gccttgattt cgccaacctt ctcattcaga aggatttcgt gtggcagctt
     3181 gccgtcgaga atctgctggc ggatttccgg cacgcacttc gggcagagtg aatcggtcgt
     3241 acggggccag cccagaggcg gtttttcctt ttcatacgac ttcaaaagcg gcttgtcaga
     3301 ccatttggga gtgaaagaag ggttggggct gatgcggttc agtctctcga agacggccca
     3361 cgcaccattc gctgcgatag tgaccgcccg ttctgcgtat ttgacggctt tcgacatttt
     3421 ttcgatctcc tgatgctttc tggcccgctg acatcatgat gcaaaagcat ttggggcaga
     3481 aagccaagct taaatgctta ccggccctcg ggggaagtgt gagtttaggt aactgccgct
     3541 tccagaggac tttaccagca ttcagcctag tcccggggag gcaaaagagg aatccctttg
     3601 ggccgaatgg ggaaatttcg tgttcagacg ggaatggtca ggggcgaacg ggaagggaga
     3661 aacctgctgc tccacccatc ctgcccgggg caagttgaag gacgccttcg gcgttgaagt
     3721 gaactttctt tagtgttttg tgctgaagct gtttctgatc tgatccagtt cagccgggca
     3781 agtctcatcc tcccctgttt gcattccgat cagtgtatac atcgcgaggc cagctttggc
     3841 tgaaacaacc tgttctgtga acgatgtccc cactccgacg cgtacgattg cagcaggcag
     3901 attgccgagc gtgccactgc tgtggccgta tacgctcaat cctggcctca ggcttagatt
     3961 gtgctcgatc ttggcctgaa aactttcagg agagcggaaa ctgaattctc tgattaatat
     4021 aactttgcaa ttgccgttta ctttttcggc attaaagttg gtgttcccag gctgtggggt
     4081 aacttgccag ccatcaggca gcaaaagtct taaccccagt aatttatttt catacgctac
     4141 cccgttcgac gtactttgca catccattgc agaaaggtta atacctgttg ctgtctgcgc
     4201 gaacagccca agggaaacca acgcaatgac tgggaggata atgataattg caaaagcaat
     4261 aggaacggca ggcgtgagat gccccgctat cgaagatcgc cagctatggt ttgccaattg
     4321 tgggaagctt tcaaacaggc gttttcgcgc aatgcgatag ccatccaggg caaaggcaaa
     4381 ttgcaaaagc agcaggatga aatcgaacca cgtaaaacca gggctctttg acaaggcttt
     4441 cacgcttcgg ccaaggacac caacaataag gaacatcgca atgcccttcc cgcgatcgcc
     4501 gagatagaag tagccaaacc cgtttgtcag taaattcagg aagcaggcga tgcgaggatt
     4561 tccaccgatg agataggttg aaatgccctg gttgtattcc agcgcggaaa aataagcatc
     4621 aagaaaacca aaaatataca gcgatatggc aatgaaaata gcgatgccgg acaagttaaa
     4681 ttgttttgcg atgagcgtgg caatccccat cagaagcgaa agataaaaaa acgtaaaggt
     4741 gacccatcct gcggttctgc ggtttgcata aacctggcca atcccgggga gaagaactga
     4801 aagcaggaag gcaattgctg cggattttgg cttctctggg cgttccggtt ccagcggagg
     4861 taatggcgaa tactcactca gaccaagcag agacataaag acattccaca agaatgcggc
     4921 cacagtatca tgcgagcatg cgtatgagaa gaggaaaaaa ttgcgatttg ataagtattc
     4981 ttgctcatcc cttaaaagaa aatatttgcc ttctccgtaa cgttttcagg cagcatagat
//
LOCUS       DASRBV010000119        13015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_513656, whole genome shotgun sequence.
ACCESSION   DASRBV010000119 DASRBV010000000
VERSION     DASRBV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513656"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..280
                     /locus_tag="VFA02_04700"
     CDS             107..280
                     /locus_tag="VFA02_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09179.1"
                     /translation="MMRTLMTPPVPQPKPEPPPDPEPRPGDEPDVVLPPGPDDDPEET
                     PDVGPMPPEPLPA"
     gene            complement(288..1526)
                     /locus_tag="VFA02_04705"
     CDS             complement(288..1526)
                     /locus_tag="VFA02_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU09180.1"
                     /translation="MAGLKAFRESGHIPTLFAAFLYFDFSFAVWVLNGAMGPFISEQF
                     HLTQGQIGLMVSIPTLAGALMRFPLGVLSQYIGRKKAAIVEMSAIVIALLYGFLFVKT
                     YHSVLAMGVLLGIAGASFGVALSLGSGWFPKKYKGLAMGIAGAGNSGTAVAALLAPRL
                     AQHFGWHNVYGFAALFMLLPLLVMIFMAKEPPDIEHQSLKEHLSCLWERDGWAFNFIY
                     IITFGGFLGLATFLPSFYFKQFHVSKVQAGTLTVFATLTGSGTRVLGGWLADRFGGIN
                     TLTIVFLVAIAGLFGLTTSPSLAITTLLFMLCFAALGAGNGATFQLVPLRWPITTAVA
                     GGMIGEIGALGGSILPNLLGQAKQRTGSYSPGFLIYIGIACLVLIMMRLIARQWTRSW
                     VGAGGRAHASVEAEAVTVIS"
     gene            complement(1543..3708)
                     /locus_tag="VFA02_04710"
     CDS             complement(1543..3708)
                     /locus_tag="VFA02_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZU09181.1"
                     /translation="MNAIARLRRALGLDTLQGKYAYGYDPEAGPVSASRIAEKWVKTT
                     CGYCSVGCGMLVGIRKGKAVAVRGNPDHPVNAGKLCPKGLSEHHILTAPGRATRPLLR
                     KNGTLSPVSWDEALDTMLLRIRAIQQKYGPESFGVISTGQLVTEEFYTLGKLVQLGFG
                     TRNYDGNTTLCMASAVSGYKLSFGSDGPPGAYADMEHADVILLIGANIADNHPILCQR
                     LDRNKHRTLIVVDPRVTKTAMMADIHLPIKPRSDIALLNGIAHVLIHEGLIDAEYIAQ
                     HTTGYEELATHVAEYTPERVASITGLTPETILHVARLYGRAKASFIGWTMGVNHSTQG
                     AVTVAAINNLALMTGNIGRMGAAPFSITGQCNAMGTRESGFTSSLPGYRKFESAKDRE
                     ELAQLWNIPVERIPPARGLAYPDIIEAAVAGKIKALWIIATNPAVSFPNYGLFEDAVR
                     RLDFLVVQDGFHPTPTGEFADLVLPAAIWGEKEGTFTNSERRVSKVNRLVAPPGEARP
                     DFDIFLDIADRLRVRDQLYPNWRGPEDAFREWQRVTAGRLCDYSKFSWKEIEERGGVQ
                     WGGPRLYQDGIFQHPDGKARLYAVPWEPFIEQPDEEYPFVLNTGRTVEHWHTRTKTAN
                     VALLHNMVPNAWLEMNPSDASRLRLKPHDRVTIYSRRSSVKNVELRITGIVAPGQVFM
                     PFHFAESNSNLVTLGAYDPISREPNFKQCAVRVERSTPN"
     gene            complement(3705..5216)
                     /locus_tag="VFA02_04715"
     CDS             complement(3705..5216)
                     /locus_tag="VFA02_04715"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZU09182.1"
                     /translation="MSLPLHERIGTDENFVRVTLAEMDVPPPQSTSLVPDRLPANGEQ
                     YRFHFDMTKCIGCRSCEVACNEQNGNPAAIHWRRIGEIEGGAWPNTKRHYLSMGCNHC
                     LDPDCLKGCPVNAYTKDPMTGIVLHSAEACIGCQYCVWNCPYSVPQFNPERGVVGKCD
                     MCYGRLADGFEPACVNACPEKAIEVEIVNIAEWRADYSAAESPGMPAAGQTISTTRIT
                     LPENTATVLERVDTDRIHPEHPHLSLVAMTTLMQAIIGTLVGALLLQGAGKLAFTALL
                     LATGIALNISVFHLGRPAYAWRALKMWRRSWLSREVLLFGLFSGALFMATALSWLPRS
                     PHWAILSFAWMAALLGIAGNTASAFIYLVPARPAWNMVHTPIDFLMSAALLGFASAPL
                     LSANPAGSSAWPVILAGTLWLANFGAKLVRLGRASLFEKRASAALLNTPLLRALLFTS
                     CGLVALCMVLTANHLNVAAALAAVTGVLLSRCLFFISVVPLNMALTFVRGGGE"
     gene            complement(5309..7111)
                     /locus_tag="VFA02_04720"
     CDS             complement(5309..7111)
                     /locus_tag="VFA02_04720"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HZU09183.1"
                     /translation="MTQMVPFVPDDAPFSKEQRAWLNGFLAGMFSSAPVPEIAAKPAP
                     LKIAVFYGSQTGTAEGLARKLTKELKAKGFDATLTSLEAYTSAALALERYAIFLVSTY
                     GQGEAPDSAQFFYEQVCVEHFPRYENLQYAVFALGDRHYETFCKFGIDLDNKLLSLGA
                     TRILDRVECDVETDGPFARWKDELFKRLDAISKGGQQPAAATPEMPAMPPSVAIASPA
                     LVREAPPATVTRDNPYLAPLKEKLPLTRDGSTKTTLHLTWSIAESGIRYEPGDACGII
                     PQNDPALVEDILNLLRFSGEEQVEIQKAGQVSLKDALRHHLVITKLNRKLIERYATIG
                     QCKRLLGLLAPEQQTHLEQYVYERGLIDILEECPGVIHNPVDLVTMLPRLTPRLYSIS
                     SSPSVHAGEVHTTIAVVRYRSLNRERGGVCSTLVADRVPVGERVPIYIQPNKKFRLPE
                     NPDTPVIMIGPGTGIAPFRAFLHERRARGAKGKNWLFFGDRNAANDFLYREELESMVA
                     DGHLTRLDTAFSRDQEHKIYVQDRMLENAPLFWSWLQDGASIYVCGDASRMAKDVDAA
                     LHTIVSRQGNMDHDGAKEYVRQLKDDHRYHRDVY"
     gene            complement(7117..8901)
                     /locus_tag="VFA02_04725"
     CDS             complement(7117..8901)
                     /locus_tag="VFA02_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HZU09184.1"
                     /translation="MATQVFTNTPDFTEEQKYYLQGFFAGITQRGKLPYVGHTATGLI
                     THDPASGGANQAAKSDEAEETIFGTPLSDLSKEERWKYEENPLDIWDKILAHANENRP
                     PSGGDVFRFKFHGLFYVAPAQDSFMLRLRIPGCILHSHQMRGLAQMAEDWGSGRADIT
                     TRGNMQIREFQPKDIVRVLNKLQTLGLTSRGSGADNIRNITASPLTGIDPVELYDVAP
                     LAEALHFYILNSRDMYGIPRKFNVAFDNGGSLSVLADTNDIGFIATHVDEGNDIPAGI
                     YFRVLLCGITGHKQFATDCGVLLTPDQTVAVAAAMVRVFNENGDRTDRKKARLKYLID
                     RWGVEKFLEETEKRLAFPLLRFPAETCAPRRPIDRTGHIGVHPQKQPGLHYIGIAVPV
                     GKLPAAQMRALAEVADHFGTGEMRLTVWQNLIIPNIRTQDVEAATAAIHAAGLKTTAG
                     TVMRGTVACTGNKGCRYAATDTKHHAVELANFLDERFVIDQPVNLHVTGCPHSCAQHY
                     VGDIGLLGATVQGEEGYQVVVGGGADNEQGLARELIPSIKATDLPPVMERLFTAYMER
                     RDAGESFLSFTRRHDIETLKSFCMEKAV"
     gene            complement(9101..10588)
                     /locus_tag="VFA02_04730"
     CDS             complement(9101..10588)
                     /locus_tag="VFA02_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZU09185.1"
                     /translation="MKRLFRCALFLLGVAIAGVPAASIAQSKPSGGLPFSVSVYDRAR
                     VNAWQWFAAPPESETYGYLENLLRIGIAQRLQHWDWQLEVAQPSVLWAPDNAVSPITA
                     QGQLGLGATYYASNSNNSNAAAAFFKQGFLRYHFNGTDKNVRLGRFEFIDGQEMQPKN
                     ATIAWLQANRITHRLIGNFGFSTAQRSFDGADAHFGSSSWDITGFAARADQGVFNMNG
                     NPELNVDLQYLAFTQTTAKQHVLWRAFALGYHDGRTGITKTDNRALAVRAKDHGNIRI
                     GTYGGDLLAAVPVGAGQLDFLFWGAVQNGSWGKLDHSANAVALEGGYKFSKAAPSPWL
                     RAGWFRGSGDKNSADNTHGTFFQVLPTPRIYARMPFYNLMNSTDSFIQVAEKPVGKLA
                     LRTDLHWLQLTSGKDLWYLGGGAYDNKVFGYQGRPGNNSTSFASMFDMSADWQTTKNI
                     GLNFYYAHAWGKQVIEKIYPANHDAQYGYAELVYRFDVEQRGAKK"
     gene            complement(10669..11508)
                     /locus_tag="VFA02_04735"
     CDS             complement(10669..11508)
                     /locus_tag="VFA02_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HZU09186.1"
                     /translation="MDYVKPVQVAESFLANAVSKSQLPVGQLLMRGFLSGALLGFATT
                     IAFTSTSQSLPPLVGAILFPVGFAMIVVLGLELVTGSFAMLPTAWIAGKIGLPRVLAN
                     LALVFLGNLAGGLFYAWMYAMVITQFHHTPVAGAGALIVAAAQAKTLAYQKLGAAGIG
                     LSVVKAMLCNWMVCMGVVMGLTSRSTLGKIVACWLPIFTFFALGYEHSVVNMFIIPAG
                     MLMGAPVSLRDWWLWNQVPVTLGNLLGGLLFVGLPMLWMSGAELKSESAIASAAQPEV
                     VEV"
     gene            11838..>13015
                     /locus_tag="VFA02_04740"
     CDS             11838..>13015
                     /locus_tag="VFA02_04740"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZU09187.1"
                     /translation="MAHASFNGLRVLSLESRRATEVAKLIRTYDGEPIVVPSMREVPA
                     STNKQAFEFADRLIHRDFDLVVFLTGVGVRTLVNVVETRYDREAFLGALRTVKVASRG
                     PKPSSVLKEFKVPIAVTAPEPCTWREVLSSLDHAFGDSLKQFRVAVQEYGASNPELLT
                     GLTERSKELTKVPVYQWALPEDVQPLREAVLAIANGGIDIALFMTAVQVIHLFQVAEQ
                     MNYVEELRRGLQSIAVVSIGPTTSEELRHYGITPDFEPSHPKMGFLVNEAAQYAGRLI
                     EEKRNKNPDAAPSPLQIQRVPRVAPSTPAMAGFRDGLAAFDFLHEISSRIASADAFHA
                     VLDRIVDFVSSVIPCDSCFIYVLEQDKLVLRASKNPHADVVDRLDVRLGQGITGWVAQ
                     "
BASE COUNT         2888 a         3613 c         3683 g         2831 t
ORIGIN      
        1 agaaccagga aggctcactc gcgattacga ttgaagaaaa tgcacaggtt tcgtggggac
       61 cgcgcctgtc tgcatccaag taatcatggc aggatccagt tcgtacatga tgcggacgtt
      121 gatgacccct ccggtcccgc aacccaagcc ggaaccgccg ccagacccgg aaccacggcc
      181 gggcgatgag ccggacgtgg tccttccgcc cggcccggac gatgatccgg aggagacgcc
      241 ggacgttggc cctatgccgc cggaacctct tccggcatag ttgttgttta gctaataacg
      301 gtaacggctt cggcttcaac gctggcgtgc gcacgtccgc ctgctcccac ccaggagcgt
      361 gtccattgac gcgcgatgag gcgcatcatg atcagcacca gacatgcgat accgatatag
      421 atgaggaagc cgggcgagta ggacccggtg cgctgcttcg cctgccccag caggttgggc
      481 aggatggaac cgccgagcgc gccgatttct ccgatcattc ctccggcaac ggccgttgta
      541 atgggccagc gcagcggtac gagctggaag gtcgcgccat tgcctgctcc cagcgccgca
      601 aaacaaagca taaagagcag tgtggtgatg gccagtgatg gcgaagtggt aaggccgaag
      661 agaccggcaa tggcaaccag gaagacaatc gtgagggtgt tgataccgcc aaagcggtcg
      721 gctagccatc cgccgaggac gcgcgttccg ctgccggtca gagtggcaaa gacggtcagt
      781 gttccggcct gcactttcga aacatggaac tgcttgaagt aaaacgacgg cagaaaggtg
      841 gccagcccaa gaaatccgcc aaaggtgatg atgtaaatga aattgaaggc ccatccatca
      901 cgctcccaca ggcagctcag gtgttctttc aggctctggt gctcgatgtc tggcggctct
      961 tttgccatga agatcatgac cagcaggggc agcagcataa agagcgcggc aaatccatag
     1021 acgttgtgcc agccaaaatg ctgggcaagc cgcggggcca gaagggccgc aactgcggtg
     1081 ccgctgttcc ctgctccggc aatgcccatg gccagcccct tgtatttctt cgggaaccat
     1141 ccggagccaa gcgaaagcgc cacgccgaag ctcgccccgg caatgcccag caggacgccc
     1201 atcgccagca cactgtgata ggtcttcacg aacagaaacc cgtatagcag cgcgatgaca
     1261 atcgctgaca tctcaacaat cgcggccttc tttctgccga tgtactggga gagaacgccg
     1321 agtggaaagc gcataagcgc cccggccagc gttgggattg agaccatcaa cccaatctgg
     1381 ccctgcgtaa gatgaaactg ctcggagatg aacggcccca tcgcgccatt cagcacccac
     1441 actgcgaagc tgaaatcgaa atataagaaa gcagcaaaca acgtggggat gtgccctgat
     1501 tcccggaatg ctttcagtcc tgccattgtt ttgttttctc ctctagttcg gtgtcgagcg
     1561 ctctacgcgc acggcgcact gcttaaaatt tggctcgcgc gaaatcggat cgtatgctcc
     1621 gagcgtgacc agatttgagt tcgattccgc aaaatgaaac ggcataaata cttgtcccgg
     1681 cgcaacaatt ccagtaatgc gcagctcgac attttttaca ctgctgcgac gcgagtaaat
     1741 ggtgacacgg tcgtgcggct ttagccgcag gcgtgacgcg tcggaaggat tcatctcaag
     1801 ccaggcgttt ggcaccatat tgtgcagcag tgcgacattc gcggtcttgg tgcgcgtatg
     1861 ccagtgctcg acggtgcggc ccgtgttcag aacaaaggga tactcttcat cgggctgctc
     1921 aatgaacggc tcccatggaa ctgcgtagag acgcgcctta ccgtcaggat gttgaaagat
     1981 cccgtcttga tacaaacgcg ggccgcccca ttgcactccg ccgcgctctt cgatttcctt
     2041 ccatgaaaat ttgctgtaat cgcagaggcg tcccgccgta acgcgctgcc actctctgaa
     2101 tgcgtcttcc ggtccccgcc agtttggata aagttgatcg cgcaccctga gacggtctgc
     2161 gatgtccagg aagatgtcga agtccgggcg cgcttctccg ggcggagcga cgaggcggtt
     2221 gactttgctg acgcggcgct cggagttggt gaaggtacct tctttttctc cccagatggc
     2281 ggcgggcaaa accaggtcag caaactcgcc tgtaggcgtg ggatgaaagc catcctggac
     2341 gacaagaaaa tcgaggcggc gcactgcgtc ttcaaacagg ccgtaatttg gaaatgaaac
     2401 ggcgggattc gtcgcgatga tccagagcgc cttgatcttg cccgcgactg cggcctcgat
     2461 gatgtctgga taagcaaggc cgcgcgctgg tgggatgcgc tcgaccggga tattccagag
     2521 ctgtgccaac tcttctcggt ctttggcgct ctcgaatttg cggtatcccg gcaaactgga
     2581 tgtaaagccg ctttcacgtg tgcccattgc attgcactgt ccggtaatag agaacggtgc
     2641 tgcacccatg cggccgatgt tgcccgtcat cagagcgaga ttattgatag cagcaactgt
     2701 gaccgctccc tgcgtagagt ggttgacgcc catcgtccat ccaatgaacg atgccttcgc
     2761 cctgccatac aaacgcgcaa cgtggaggat ggtctctgga gtgaggccgg tgatggaagc
     2821 aacacgctcg ggcgtgtatt cggcaacatg cgtggcgagt tcttcgtatc cggtggtgtg
     2881 ctgcgcgatg tattcagcgt ctatgaggcc ttcatgaatc agaacgtgtg cgatgccatt
     2941 gagcagtgcg atgtcgctgc gcggcttgat gggcagatga atgtctgcca tcattgctgt
     3001 cttggttaca cgtggatcaa caacgataag tgtgcgatgt ttgttgcgat cgagccgttg
     3061 acacagaatc ggatgattat ctgcgatgtt cgcgccaatg agcagaatga cgtcggcatg
     3121 ctccatatcg gcatacgcgc cgggagggcc atcgcttccg aacgagagct tgtatccgct
     3181 gacagcgctc gccatgcaaa gagtagtgtt gccatcgtag ttacgcgttc cgaagccaag
     3241 ctgcacgagt ttgcccagtg tgtagaactc ttctgtgaca agctggcctg tgctgatgac
     3301 gccgaaggac tccgggccat atttctgctg aatcgctcga atgcgcaaga gcattgtgtc
     3361 cagcgcttcg tcccatgaaa caggggacag cgtgccgttt ttgcgcagca atggcctggt
     3421 cgcccgtccg ggcgcggtga gaatgtggtg ctcacttaac cctttcggac acagcttgcc
     3481 tgcgttcacc gggtggtcag gattgcctcg tacagcgacg gccttgcctt tacgaatgcc
     3541 cactaacatt ccgcagccta cggagcaata gccgcaggtg gtcttcaccc acttttcagc
     3601 gatgcgcgaa gcggagacgg gtccggcctc ggggtcgtat ccataggcat atttaccctg
     3661 gagcgtgtcc agcccaagcg cccggcgcag gcgcgcgatt gcgttcattc tccgcctccg
     3721 cgcacgaaag tgagggccat gttgagcggc acaaccgaaa taaagaagag acagcgcgag
     3781 aggagcaccc ctgtaactgc tgccaaagca gcggccacat tcagatgatt ggcggtcagc
     3841 accatgcaga gggccaccag accacatgat gtaaacagaa gtgctcgcaa tagcggcgta
     3901 ttcagcaggg ctgcgctggc gcgtttctca aagagtgagg cgcggcccag ccgcacgagc
     3961 tttgcaccga agttggccag ccagagtgtt ccggcaagta tgacgggcca tgcagaagat
     4021 ccagcaggat tggctgaaag cagaggcgca gaagcaaacc caagcagcgc agcgctcata
     4081 agaaaatcta tgggcgtatg caccatgttc cacgccgggc gggccggaac gagatagatg
     4141 aacgcgctcg cggtgtttcc ggcgatgccg agaagcgcgg ccatccatgc aaaactgaga
     4201 atggcccagt gcggtgaccg cggaagccag gacaatgccg tggccatgaa cagcgcgccg
     4261 gagaacaatc cgaacaggag cacttcgcgg ctcaaccatg aacgccgcca cattttgagg
     4321 gcacgccatg cgtacgctgg cctgccgaga tggaagacgg agatgttcag tgcgattccg
     4381 gttgcgagca gtaatgctgt gaatgcaagc ttccctgcac cttgaagcaa aagcgcgcca
     4441 acgagtgtgc caatgatggc ctgcatcagc gtagtcatgg ccacaagtga caaatgcgga
     4501 tgctcaggat gaatgcggtc tgtgtctacg cgctccagga cagtcgccgt gttttccggc
     4561 agcgtgatgc gcgtggtcga aatggtctgc ccggctgcgg gcattccggg agattccgca
     4621 gccgagtagt ccgcacgcca ctctgcgatg ttcacaatct caacctcgat tgccttctca
     4681 ggacacgcat tgacgcacgc aggctcaaag ccgtccgcga gacgcccgta acacatgtcg
     4741 cacttgccga caacgccgcg ctcaggatta aactgcggaa cactgtaagg acaattccag
     4801 acgcagtact ggcagccgat gcacgcttct gccgaatgca ggacaattcc tgtcatcgga
     4861 tctttggtgt atgcattgac ggggcatcct ttcaggcagt cggggtccag gcaatgattg
     4921 cagcccatcg agagataatg ccgctttgta ttgggccatg cgccgccttc aatttcgcca
     4981 atacgccgcc agtggatcgc cgccggattg ccattctgct cattgcatgc aacctcgcac
     5041 gaacggcagc cgatgcactt ggtcatatcg aagtgaaagc ggtactgctc accatttgca
     5101 ggcaggcggt ctgggacaag cgatgtgctc tgcggcggcg gcacatccat ttcggcaagc
     5161 gtcacacgca cgaagttttc gtctgttcca atccgttcat gtagcggcaa tgacacgtgt
     5221 gcgttccttt caatcgggac tcgactgaat agactccggc tgtcccatat ctcgcggaga
     5281 tccgggtttg ccctgcattg gatgttcgct agtacacgtc gcggtgatag cgatggtcgt
     5341 ctttcaactg gcgcacgtac tccttcgcgc catcgtggtc catgtttccc tgtctggaaa
     5401 caatcgtgtg cagcgcggcg tccacatctt ttgccatgcg cgatgcatcg ccacaaacat
     5461 agatgctggc accgtcctgc agccacgacc agaacaacgg agcattttcc agcatccggt
     5521 cctgcacgta aattttgtgc tcctggtcgc gcgaaaaagc agtgtccaga cgtgtgaggt
     5581 gtccatcggc aaccatgctc tcgagttctt cacgatatag aaaatcattg gccgcgttcc
     5641 ggtcgccgaa gaagagccag tttttgcctt ttgcaccacg cgcgcggcgc tcatgcaaaa
     5701 aggcgcggaa aggcgcgatc cctgttcccg gaccgatcat gatgacggga gtgtccggat
     5761 tttccggcag ccggaacttc ttgttgggct ggatgtaaat cggcacccgc tcaccgacag
     5821 gtacgcgatc tgcaaccaac gtagagcaca cgccgccgcg ctcgcgattt aaagagcggt
     5881 agcgcacgac agcaatagtg gtatgtactt ctccagcgtg cacagatggg ctggaagaga
     5941 tggaatacag acgtggcgtg agccttggca gcatggtgac gaggtccacg ggattgtgaa
     6001 tcactcccgg gcactcttcc agaatgtcaa tgaggccgcg ctcgtaaaca tattgttcaa
     6061 gatgcgtctg ctgctctggc gcaagcaggc cgagcaaccg cttgcactgg cctatcgttg
     6121 cataacgctc gatgagcttg cggttcaatt ttgtaatcac caggtgatgg cgcagggcat
     6181 ccttcagaga gacctgcccg gctttctgga tttcgacctg ctcttctccg gagaagcgca
     6241 gcaaattgag aatgtcctca acgagtgcag gatcgttctg cggaataatt ccgcaggcat
     6301 cacccggttc atatcttatg cccgattcag cgatggacca ggtgagatgc agcgtcgttt
     6361 tggttgagcc atcgcgcgtg agcggcagct tttccttaag cggagcgagg tagggattgt
     6421 cgcgtgtgac ggttgcgggc ggtgcttctc tcaccagtgc cggagacgca atcgcaaccg
     6481 acggcggcat tgccggcatt tcgggagtgg ctgctgcggg ctgttgacct cctttggaga
     6541 tggcatcgag ccgtttgaac agttcgtcct tccagcgcgc aaacggtccg tctgtttcta
     6601 catcgcactc cacgcggtcg agtatgcgtg ttgcgccaag cgacaagagc ttgttgtcga
     6661 ggtcaattcc aaacttgcag aaggtttcgt aatggcggtc accaagagca aagactgcgt
     6721 attgcagatt ttcgtagcgc ggaaagtgct cgacgcagac ctgctcgtag aagaactgcg
     6781 cagaatccgg cgcttctccc tgaccatagg tgctgacaag aaagatggcg taacgctcca
     6841 gcgcgagcgc ggcagacgta tatgcttcca gcgatgttag tgttgcgtca aagcctttgg
     6901 ccttcagttc ttttgttaat ttgcgcgcca gaccttcagc cgtaccagtc tgcgagccgt
     6961 aaaagacagc aatcttcagc ggtgcaggtt tcgcggcaat ctccggtacg ggggcgcttg
     7021 aaaacattcc ggcaagaaag ccattgagcc aggcgcgctg ctcttttgag aaaggtgcgt
     7081 cgtcgggaac aaatgggacc atttgcgtca tcgctgctaa accgcctttt ccatacagaa
     7141 ggatttgaga gtttcgatgt catgtctgcg cgtgaatgag aggaaggact ctcctgcatc
     7201 acgccgttcc atgtatgcgg taaagagtcg ctccatgacc ggaggcaggt ctgtggcctt
     7261 gatggaggga atcaattcgc gcgcaaggcc ctgctcattg tccgcaccgc ctccgaccac
     7321 gacctggtag ccttcttcgc cctgcactgt tgcaccgagt aggccgatgt cgccgacata
     7381 atgctgtgcg caggagtgcg ggcagcctgt aacatgcagg ttcacgggct ggtctatgac
     7441 gaagcgctca tcaagaaagt tggccagttc tactgcgtgg tgcttggtat cagtagcggc
     7501 atagcggcaa cctttgtttc cggtgcatgc gaccgtgccg cgcatgactg tgcctgccgt
     7561 ggtcttgagt ccagccgcat gaatggccgc tgtagccgct tccacatcct gcgtcctgat
     7621 attgggaatg atgaggtttt gccatacggt aagccgcatc tcccctgtgc caaaatggtc
     7681 agcgacttct gccaacgcgc gcatctgcgc tgcgggcaat ttgccaacag gaacagcaat
     7741 accgatgtaa tgcagcccag gctgcttttg cgggtgcacg ccgatgtgtc ccgtgcggtc
     7801 aattggcctg cgcggtgcgc acgtttccgc aggaaatctc agcagcggaa acgcaaggcg
     7861 cttttctgtt tcttccagaa atttttcaac accccagcgg tcaattaaat atttgagccg
     7921 tgcttttttg cggtcagtac ggtcaccgtt ttcattgaag acccgaacca ttgcagcggc
     7981 gactgcgaca gtctggtcag gggtcaggag cactccgcaa tcagttgcga actgtttatg
     8041 cccggtaata ccgcacagca gtacgcggaa atagatgcct gccgggatgt cattgccttc
     8101 atctacgtgg gttgcaataa agccaatgtc gttggtgtcc gccaacacgc tgagcgagcc
     8161 gccattatca aatgcaacat tgaacttgcg cgggattcca tacatatcgc gcgagttgag
     8221 gatgtagaag tgcagggcct cggccagcgg ggccacatcg tagagttcga ctggatcaat
     8281 cccggtgagg ggcgaagcag tgatgttgcg aatgttgtcc gcacccgagc cgcgcgaagt
     8341 cagcccgagc gtttgcagct tgttcagcac gcggacgatg tccttcggct gaaattcgcg
     8401 gatctgcatg ttgccgcgtg tcgtaatgtc cgcgcggcca gagccccagt cttcggccat
     8461 ctgggccagc cctcgcatct gatgcgagtg cagaatgcaa ccgggaatgc gcagccgcag
     8521 catgaaggag tcctgcgccg gagccacgta aaagagaccg tgaaacttga agcgaaaaac
     8581 atctccacca gagggcggac ggttttcatt tgcgtgcgcg agaatcttgt cccaaatgtc
     8641 gagcggattc tcttcatact tccagcgttc ttctttgctt agatcagaca gtggtgtgcc
     8701 gaagattgtc tcttccgctt catcggactt ggcggcctgg tttgcaccac ctgaagcagg
     8761 atcgtgcgtg atcaggccgg ttgctgtgtg cccgacataa gggagcttgc cgcgctgcgt
     8821 gattccggca aagaaaccct gcagatagta cttctgctct tctgtgaaat ccggcgtgtt
     8881 ggtgaacacc tgtgttgcca tgacagaaat ccttgtttcg atgtaaatat gcgttcgctg
     8941 aatcgagcag ctgttcaaag ctcttcacgg agcgcagtaa gttgttcagg aatttctgcc
     9001 atgacacggc gtggtgtcag gcggacttga tgattttgat catacttcgg ctgtttttgc
     9061 gaagcaagat gcgtccggaa caaatacgcg gtttttcgca ttatttcttt gctcctctct
     9121 gctccacatc aaagcgatat accaattctg cgtatccgta ttgcgcatcg tgattggctg
     9181 gataaatctt ttctatgacc tgttttcccc atgcatgcgc gtaatagaag ttcaaaccaa
     9241 tgttctttgt cgtctgccag tctgcgctca tgtcaaacat ggaagcgaac gatgtactgt
     9301 tgtttcccgg gcggccctgg tagccgaaaa ccttgttgtc ataggccccg ccgccgagat
     9361 accagagatc ttttcctgaa gtaagttgta accagtgcag gtctgtgcgc agggccagtt
     9421 ttcctactgg tttttcagct acctgaataa agctgtctgt gctgttcatc aggttgtaga
     9481 aaggcatgcg cgcgtagatg cggggcgtag gcaggacctg aaagaacgtt ccgtgcgtat
     9541 tgtcggcaga gttcttgtcg ccgcttccgc gaaaccatcc ggcacgcagc cacggggacg
     9601 gtgcggcctt gctgaacttg tatccacctt cgagggccac ggcattggca ctgtgatcaa
     9661 gcttgcccca gcttccgttc tgcactgctc cccagaagag aaaatccagt tggcctgcgc
     9721 ctaccggcac ggcagcgagc aaatccccgc cgtaagtccc aatacgaatg ttgccgtggt
     9781 ctttagcgcg tacagccaga gcgcggttgt ctgttttggt gatgcctgtg cgtccatcgt
     9841 gatagccaag ggcaaaggcc cgccacaaga cgtgctgttt tgccgtggtc tgcgtaaagg
     9901 caagatactg caagtccaca ttcagctcag gattgccgtt catgttgaag acgccctggt
     9961 cggcgcgggc tgcgaaccct gtgatgtccc atgaactgga gccgaagtgc gcgtcggcac
    10021 cgtcaaaact gcgctgggca gttgaaaagc caaagtttcc aatcaggcga tgcgtgatgc
    10081 ggtttgcctg tagccaggcg atagtcgcgt ttttcggctg catctcctgt ccatcaatga
    10141 actcaaaacg cccgaggcgc acgtttttgt ccgtgccgtt gaagtgatag cgcagaaatc
    10201 cctgtttgaa gaatgcggcg gctgcattgc tgttgttgct attcgaggca tagtaagtag
    10261 cgccaagccc aagctggccc tgcgcagtta tgggggaaac agcgttgtct ggcgcccaaa
    10321 gtacagaggg ctgcgcaacc tctaattgcc agtcccaatg ctgaagccgt tgcgcaatcc
    10381 ctatacgcag aaggttctca agatatccat aggtctcaga ctctggcgga gcagcaaacc
    10441 actgccatgc attcacacgc gcgcggtcat agacagaaac gctgaatggc aacccacctg
    10501 aaggtttgct ctgcgcaatg gaagctgccg ggaccccggc tatcgctact cccagtaaaa
    10561 acaaagcaca tcgaaatagt cttttcataa tctcttgccc ccataaaatt gagtgattca
    10621 gatacgcctc ttccttcttc ctgctgattg cgaaggaaca gcctgtcttc agacctctac
    10681 aacctcaggt tgtgcagcag aagctattgc tgactcagat ttaagctctg ccccgctcat
    10741 ccacagcatg ggcaggccaa caaaaagtag gccacccaac agatttccca acgtgactgg
    10801 aacctggttc cataaccacc agtcccgcag agacacaggc gcgcccatca gcattcctgc
    10861 cgggatgatg aacatattta cgaccgagtg ctcgtatccc agggcgaaga aagtaaagat
    10921 gggcagccag caggcgacga tcttgcccaa agtcgagcgc gaggtcaggc ccatcacaac
    10981 gcccatgcaa accatccagt tgcagagcat cgctttgacg actgacaatc cgatgcctgc
    11041 cgcgcccagc ttttgatacg ccagcgtctt ggcctgagcg gccgccacaa tcagcgcgcc
    11101 tgctccagca accggggtgt ggtgaaactg cgtgatgacc attgcataca tccacgcgta
    11161 gaaaagccct ccggccagat tgccgagaaa gaccagagca aggtttgcca gcacgcgcgg
    11221 caggccaatt ttccctgcaa tccatgctgt tggcagcatg gcgaagctgc ccgtcaccag
    11281 ttcgagtccc agaacgacga tcattgcgaa accaactgga aagagaattg caccaacgag
    11341 cggcggcagg ctttgcgacg tagatgtgaa tgcaatggtg gtcgcaaatc caagcaatgc
    11401 gccggaaagg aatccgcgca tcaacaattg ccccacaggc aactgcgact tgctgacggc
    11461 gttggccaga aagctttccg caacttgcac gggtttgacg taatccatag acactcctga
    11521 tgggtcgtga aagtgataca cgcatgcaca gaactgatcg gtgcagaaaa aatttctttg
    11581 gattgctcaa cggagagcag gagaaagaac agtggacact gcgaggtgcc cttcggaact
    11641 gttgctttca ttgagcaata ccatggtgca aatgtgattg caatgaagtt ttcgtctcaa
    11701 agcgcgaacg aaaagacttt tatggaagca ataaacaaat tcaattccag atggccttga
    11761 caacgattca tgacaagata gaactatcca ggcggccacg taagccgccc gtccagcaaa
    11821 accgcaggag gacaaccatg gcgcatgcaa gcttcaacgg acttcgtgtc ctgtcgcttg
    11881 aatcccgccg cgccacagaa gttgccaagc tcatccgtac ctatgatggc gaaccgatcg
    11941 tcgtgccttc gatgcgagaa gtccctgcat ctacaaacaa acaggcgttt gagttcgctg
    12001 accgcctgat ccatcgcgat ttcgatcttg ttgtttttct tacgggtgtc ggcgttcgca
    12061 cactggtaaa cgtggttgaa accaggtacg atcgcgaagc gtttcttggc gctcttcgca
    12121 cggtgaaagt agcaagccgg ggccccaagc catccagtgt tttaaaggaa tttaaagtac
    12181 cgatcgccgt gactgcgccc gagccatgca cctggcggga agtgctgtca tcgctcgatc
    12241 atgcttttgg agattcgctt aaacagttcc gtgttgctgt acaggaatat ggcgcgtcga
    12301 atccggagtt actcacgggc cttacagaac gcagcaaaga gctcaccaag gtccccgtct
    12361 atcagtgggc cctgccggag gacgtgcaac ctctgcgcga agccgtactt gctattgcaa
    12421 atggcggcat agatatcgcc ctcttcatga cggccgtaca ggtgatccac ctgtttcagg
    12481 ttgccgaaca gatgaactat gtagaagagc tgcggcgcgg actacagtcc attgcagtgg
    12541 tctccattgg accgactaca tcggaagagt tgcggcatta tggcatcaca ccggatttcg
    12601 agccgtccca tccaaagatg ggctttctgg tgaatgaagc agcacagtat gcaggacgtc
    12661 tgatagaaga gaagcgcaat aaaaatccgg atgcggcccc atcacctttg caaatacaaa
    12721 gagtgccgcg cgttgcgcct tctactccgg ccatggctgg ctttcgcgat ggcctcgccg
    12781 cctttgattt tctgcacgaa atttccagtc gcatcgcgtc ggctgacgcc ttccatgccg
    12841 ttttggaccg tatcgttgat ttcgtctcca gtgtcatccc atgcgattcc tgcttcatct
    12901 acgtgctcga acaggacaag ctggtgctgc gcgccagcaa aaaccctcat gctgatgtag
    12961 tggacaggct cgatgttcgt ctgggccagg gcatcacagg ctgggtagcg cagca
//
LOCUS       DASRBV010000120         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_513705, whole genome shotgun sequence.
ACCESSION   DASRBV010000120 DASRBV010000000
VERSION     DASRBV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513705"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..2015)
                     /gene="dnaG"
                     /locus_tag="VFA02_04745"
     CDS             complement(201..2015)
                     /gene="dnaG"
                     /locus_tag="VFA02_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZU09188.1"
                     /translation="MSGHAVPYKRETKFHTNVLYLVSDFAQSVKQQADIVKIIGEYVR
                     LRKAGAQNYTGLCPFHKEKTPSFSVHITRQIFYCFGCHEHGDVFTFIQKIENISFPEA
                     VRFVAQKCGIPLPKREFSSEEEAKEGRLRAKLLELHELACAWFEEQLRSPEGALAREY
                     LSGRGLRPEDIKTFRLGYAPDNFHALRERLQSVADTETLRLSGLFSFKEQEDGSPGPL
                     YSRFRKRVTFPIANESGRIIAFTARALEAGEKAGPKYLNSPETPLYSKSNVLFNLDKA
                     RQAIRELNFAVLVEGQMDCISAYLAGVRNVIATSGTAFTESQVRLLGRYTKQVIVNFD
                     PDTAGANAAEKSIALLTEEDFNVRVVTLEGGLDPDRYIREHGAQAYIAALRTAKRHQD
                     YLVERARQLFPPNTANAKIEALNFLLPHIRRVPSRIARDEFAADAAQKLGIDSALVRE
                     ELKQAAAKRRDSLSNTMPQLSEAERMMLRALASSQTDAVFQAAAAGFDANEAHFSGLT
                     TSHILAQLRHRDSQSPMDAVPDPRDRSLLAQALMDELTPLDKEHIAAALETLRRLYFE
                     RRQRELRAAISEAERRGDTTSVLQLTAEKISVDRALRE"
     gene            2127..4094
                     /locus_tag="VFA02_04750"
     CDS             2127..4094
                     /locus_tag="VFA02_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZU09189.1"
                     /translation="MNLLLRGFLLLSLTSIALFSATASDFQQPTREELSMTSDPAAPG
                     SDAVYLFRQETSDDKLHFHSLYARIKILTEKGKKYADVEIPYEGRTFTIRAVEGRTIH
                     PDGTAIPFTGKPIDKLLFKSGDRRIMSKVFSLPDVQVGSIIEYRYVLAYEDNIASSPQ
                     WYIQQPLFVHKAHYHFNPTTHELVSNSEHGNVVNRLLYTQVLPPGAKVRDGLDGFDLE
                     VENIPAISDEEYMPPMRSVSYRVLFYYTAAYSGDDYWKEMGKYWSKDVNRFAAPSAKM
                     QQAVQQIVSSGGTEEEKLKKIYAAVMQLENTSFTRERTAAENKALGLKIKTADDIWEQ
                     KRGNEDELTRLFIAMVRAAGMKAYDMIVTNRDRDLFVKSYLDWDQLDDEIAIVPVNGK
                     EMFFDPGQRYCEFGKLHWKHTMVQGIRQTDSGTEITNTPSPTYKDTVVSRFADLTVDA
                     EGKVSGVIRINYTGSDALYWRQFALRNDEMETKKAFEDALQRTMPPGIVVKTNHFIGL
                     ADYNTVLMALVDVSGGMGTATGKRIFLPGTFFEAGAKPLFAHEKRESPVDLHYPYMVT
                     DQVKLTLPAGMVIENTPKDTEIPLPKFADYVAKYKGSTNSYNYGRLLVMANIFYEAKE
                     YPDLRGFYAKVNAQDQQQIVLHMEAAATKEQ"
     gene            4101..>4423
                     /locus_tag="VFA02_04755"
     CDS             4101..>4423
                     /locus_tag="VFA02_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09190.1"
                     /translation="MKTLSLLGLAAWFSVAGARGSTPPVPDWVQQAASVPVTPPAGAK
                     AIVLLRDELLTVQPDGQARIRRREVIKLLRPQGREYGELFAGNGMGKKLLSFHAWSIA
                     PDGHP"
BASE COUNT          979 a         1192 c         1298 g          954 t
ORIGIN      
        1 gctacgctct gagttcttca acctctttaa cactccgcaa ttcaacaatc cgaatgccac
       61 ggttggcacg gttggttttg ggaccatctc ttcagcaggt tctccaaata cattgcagcg
      121 tgtatcgaga gaggtacagc tcgcggccaa ggtggtcttt tagaggagag gattctccgg
      181 gtatgtccaa tttctccggg tcattcgcgg agggcgcggt ctacggagat tttctctgct
      241 gtcagttgca gcacggatgt agtgtcgcct ctgcgctctg cctctgagat ggcagcgcgc
      301 agctcgcgct gccggcgctc gaagtacagg cggcgcaggg tttcgagcgc ggcggcgatg
      361 tgctctttgt cgagcggggt cagctcgtcc atcagtgcct gggcgagaag gctgcggtcg
      421 cgcgggtcgg ggacggcatc catcggcgac tgcgaatcgc ggtgccgcaa ttgcgcgagg
      481 atgtgggagg tagtcaaccc ggagaagtgc gcttcattgg catcaaagcc tgcggcggcg
      541 gcctgaaaaa cagcatctgt ttgcgatgag gcgagcgcgc gcagcatcat cctttctgcc
      601 tcgctcaact ggggcatggt gtttgaaagc gaatcgcgcc gtttggccgc ggcctgcttc
      661 agctcttcgc gcaccaaagc agaatcaatg ccgagcttct gcgcggcatc ggcagcgaat
      721 tcatcgcggg caatgcggct gggcacgcgc cggatgtgag gcagcagaaa attgagcgct
      781 tcgatttttg cattggccgt gttgggcggg aaaagctggc gcgcgcgttc tacaagataa
      841 tcctggtgac gttttgctgt gcgcagtgcg gcgatgtatg cctgcgcgcc gtgctcgcgg
      901 atgtagcggt cagggtcgag tccaccttcg agcgtgacca cccgcacgtt gaagtcttct
      961 tcagtgagga gcgcgatgga cttttcagct gcatttgcgc ctgctgtatc ggggtcaaag
     1021 ttcacgatga cctgttttgt gtagcggccc agcagtcgca cctgtgattc agtaaatgct
     1081 gtgccgctgg tggcaatcac gttgcggacg cccgccagat acgctgagat gcagtccatc
     1141 tggccctcga caagcacggc gaaattcagt tcgcggatgg cctgtcgcgc cttgtcgaga
     1201 ttaaaaagca cattgctttt ggaatagagc ggcgtctcag gcgagttcag atactttggt
     1261 ccggctttct cgcctgcttc gagagcgcgg gcggtaaagg cgatgatgcg gcccgattca
     1321 ttcgcaatag gaaatgtaac gcgcttgcgg aagcgcgaat agagcgggcc cggcgagccg
     1381 tcttcctgct ccttgaagga gaagagtccg gaaagacgca gggtttcagt atccgccacg
     1441 gattgcaggc gctcacgcaa ggcgtggaag ttgtctggtg catagccgag gcgaaacgtt
     1501 ttgatgtctt cgggcctgag gccgcgtccg gagagatatt cgcgcgccaa ggccccttca
     1561 ggcgagcgca actgctcttc aaaccaggcg caggcgagtt cgtgcagctc gagcagcttt
     1621 gcccgcaggc ggccttcctt cgcctcttct tctgaagaaa attcgcgctt cggcagaggg
     1681 atgccgcact tctgcgcaac aaagcgcacg gcctcgggaa agctgatgtt ttcgatcttc
     1741 tggataaagg tgaaaacgtc gccgtgctcg tggcagccga agcagtagaa gatctgcctg
     1801 gtgatgtgga cggagaagga cggcgtcttt tccttgtgga atggacacag tccggtgtag
     1861 ttttgagcgc cggccttcct gaggcgcacg tattcgccga tgatcttcac aatgtcggcc
     1921 tgctgtttta ctgattgcgc aaagtcagac acaagataca ggacattcgt gtggaatttc
     1981 gtttctctct tatagggtac ggcatggccg gacataccga attttttact tgccgtaaat
     2041 ggattttttc gagtaaaaca gagccactta cggtgatata ggtctcgccc cccacgaacg
     2101 cctgaatcca gcttgaggcc attcctttga acttacttct gcgcggattt ctgttgcttt
     2161 cactcacttc catcgccctt ttctctgcaa ccgcttctga ttttcagcaa cccacgaggg
     2221 aagaactcag catgacctcc gatccggcgg ccccaggttc ggatgcggtg tatctgttcc
     2281 ggcaggaaac atcggacgac aagctgcatt tccattcgct ctatgcgcgg atcaaaatcc
     2341 ttaccgaaaa agggaagaaa tatgcggacg tagaaattcc ttacgaaggc cggacgttta
     2401 ccatccgcgc cgtcgagggg cgcacgatcc atccggacgg gaccgccatt ccttttactg
     2461 gaaagcccat agacaagctg ctgttcaaga gcggcgacag gcgcatcatg agcaaggtct
     2521 tcagcctgcc ggacgtgcag gtaggcagca tcatcgaata ccgctacgtg ctcgcttatg
     2581 aagacaacat cgcgtcttcg ccgcaatggt acattcagca gccattgttt gttcacaagg
     2641 cgcattatca cttcaacccg acgacgcatg aactggtcag caacagcgag catggcaacg
     2701 tggtgaacag gctactgtac acgcaggtgc tgcctccggg ggcgaaggtg cgggacgggc
     2761 ttgatggctt cgatctggag gtagaaaaca ttccggccat ttccgatgag gagtacatgc
     2821 ctccgatgcg cagtgtctcc tatcgcgtgc tgttctatta caccgccgcc tattccggtg
     2881 acgactactg gaaggagatg ggcaaatact ggtcaaagga cgtcaatcgc tttgctgcgc
     2941 cttcggcaaa gatgcagcag gccgtgcagc agattgtttc ctccggcggc actgaggaag
     3001 aaaagctcaa aaagatttac gccgctgtaa tgcagcttga aaacaccagc tttacccgcg
     3061 agcgcacggc ggcggaaaac aaagcgctgg ggctgaaaat aaagacggcg gacgacatct
     3121 gggagcagaa gcgcggcaat gaagatgagc tgacgcgcct ctttatcgcc atggtgcgtg
     3181 ctgcgggaat gaaggcgtat gacatgatcg tgaccaaccg ggaccgcgat ctgttcgtca
     3241 agtcgtatct ggattgggac caactggacg atgagattgc gattgttccc gtcaacggaa
     3301 aggaaatgtt ttttgatccc ggccagcgct actgcgaatt cggtaagttg cactggaagc
     3361 acaccatggt gcagggtatt cgccagacag acagcggaac agaaattacc aatacgccgt
     3421 cgcctactta caaagacacg gtggtaagcc gctttgccga tctgaccgta gacgccgaag
     3481 gcaaggtcag cggcgtcatc cgtatcaact acacgggaag cgatgccctg tactggagac
     3541 agtttgccct gcgcaatgac gaaatggaga caaagaaggc cttcgaagac gcgctccagc
     3601 ggaccatgcc gccaggcatc gtggtcaaga caaaccactt catcggcctt gcggactata
     3661 acaccgtcct gatggcgctt gtagacgtca gcggcggcat ggggacggcc acagggaagc
     3721 gcatctttct gcccgggacg ttttttgaag cgggcgcaaa gcctctcttc gcgcatgaga
     3781 aacgcgaaag ccctgtagac ctccattacc cctacatggt gacagaccag gtgaagctca
     3841 ctctgcctgc cggcatggtc attgaaaata cgccgaagga cactgagatt ccgctgccga
     3901 aatttgccga ttatgtggcc aaatacaaag gctcgacaaa cagttataac tatggccgcc
     3961 tcctggtgat ggcgaatatt ttctatgaag caaaggaata ccccgacctg aggggcttct
     4021 atgcgaaagt gaatgcgcag gaccagcaac agatcgtgct ccatatggaa gcagcagcaa
     4081 caaaggagca gtaaatgcgc atgaaaacac tcagtctgct cggtttggcg gcatggtttt
     4141 ctgtcgcagg cgcccgcggc tccacaccgc ctgttccgga ctgggtgcaa caagccgcgt
     4201 ctgttccggt aactcctcca gccggagcaa aggccatcgt tcttttgcgc gacgaactgc
     4261 ttacagtaca gcccgatgga caagcgagaa tccgccgcag agaagtcatc aaactgctgc
     4321 gccctcaggg gcgtgaatat ggggaactct ttgccggaaa cggcatgggc aaaaagctgc
     4381 tctcgttcca tgcatggagc atcgcgcccg atggccatcc ata
//
LOCUS       DASRBV010000121         6272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_360594, whole genome shotgun sequence.
ACCESSION   DASRBV010000121 DASRBV010000000
VERSION     DASRBV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360594"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFA02_04760"
     CDS             <1..556
                     /locus_tag="VFA02_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09191.1"
                     /translation="NAVEVPMIGDGHLQAIAGNRNKWMIRYLPFKGEARLLTEVESSC
                     QPTEEPVSHDVTLIFTCPRSGNDHLVSAINLDGKLLWQQRWDARYIWPTFQLTQNGSR
                     FAYSSLEVSRPVGPMDPVDQDSILKQMVGVFDTASGKLLLVKNATPILSAGQNYALSP
                     DGMRFVILREGALEVYNLPPISEH"
     gene            complement(648..1457)
                     /locus_tag="VFA02_04765"
     CDS             complement(648..1457)
                     /locus_tag="VFA02_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZU09192.1"
                     /translation="MAMTALIIDDEELAREELKYLLDTTGSVEVLAQGTNGIEAVDLI
                     RTHQPDVVFLDVQMPGLDGFAVLKKLLEHGESEHLPQIIFATAYDQYAVKAFDVNAID
                     YLLKPFDRDRVLQAIERARQRLQEVHGHEKIGDLGDNASLSALLKLIEQQQQPQRHSG
                     KIVLQAHSRLFLIDQKDICFASIDEGVITVATPTLEGQSKCRTLEELLEQLDPAIFWR
                     AHRSYVVNINHIKEVVPWFKSSYQLRMDDKKQTEIPVSRAQTRRLRELFKL"
     gene            complement(1517..2059)
                     /locus_tag="VFA02_04770"
     CDS             complement(1517..2059)
                     /locus_tag="VFA02_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZU09193.1"
                     /translation="MGTNYPIRQEAETSFSNELRLIPRWSIAAAVLAFVGMEYLFWVV
                     FPRGAHHHPPIGIRLYLAITWGLLASLYMLMVGYVSNDSPRRGMSARIWIIICLVMPG
                     GIGAVLYFLLRQPIISLCPACGTRVQSEYHFCPQCAYQISASCGKCFRSVRITDVYCV
                     HCGHNLASDNRPARLHAFQD"
     gene            complement(2066..2437)
                     /locus_tag="VFA02_04775"
     CDS             complement(2066..2437)
                     /locus_tag="VFA02_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09194.1"
                     /translation="MNTQRIPDPQLIDMLAGMNTSASTPVVQRTRRAVMQAASELREQ
                     RRRNRRNIAIVSLVFIALSALLTPAIWSTIDDYFGGEQIFGLSEMITVLALVLFSAVL
                     GVLLISFRDQAPLRFGRHDRR"
     gene            complement(2434..3045)
                     /locus_tag="VFA02_04780"
     CDS             complement(2434..3045)
                     /locus_tag="VFA02_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU09195.1"
                     /translation="MSTASITLTGQAREESALIAQGLKRHDAGLLDELIVQYQHRLLR
                     YLLYLTGNRDLAEDLFQETWMRVLMRGDQFNGNARFETWLFTIARNLVIDLRRRRSMV
                     SLEELCDSSEDDRPFEVPGNDLNPFELYQSYENSRIVAELLLTLDPLHREVLVLRFHE
                     ELALEEIAQITRAPLSTVKSRLYRGLASLKPRLEAMTSGKEAQ"
     gene            3255..4058
                     /gene="rlmB"
                     /locus_tag="VFA02_04785"
     CDS             3255..4058
                     /gene="rlmB"
                     /locus_tag="VFA02_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZU09196.1"
                     /translation="MEVLYGVHPVEEALRAGSRRFDHVCFARERAGTHVDQRLQRVID
                     ACREAGVRLRFEPREHLTRLAKTPSHQGVVAVVREKATLELEDLLDAPEDGRARLFLA
                     LDGVEDPQNLGALLRSADGAGADGVVLTERRSAPLSPVAIKASAGAAEHVPIARVVNL
                     SRALEQMKERNLWCVGLDERGTMSYDEFDFTSNTVIVLGREGAGLHDLVKRHCDHLLR
                     IPMAGKVASLNVSVAGAVVLYEAARQRRAGQMKAASPPKKKEKKGLGLS"
     gene            4079..5890
                     /locus_tag="VFA02_04790"
     CDS             4079..5890
                     /locus_tag="VFA02_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09197.1"
                     /translation="MAAFLCGAAAFAQQAEQGHGKVLFSRTEDEAQKSTPEKTQTIQA
                     KATDAERSAITITRYDLDVHLMLKQQAMAAQARVTLRNDGNAPLTHLPLQVSSSLHFE
                     TIALHGKKLQFAQNLINSDADHTGQLHEAVIELPEALAPKSEITLDAGYSGEITVSTK
                     RLEQIGTPADIAKQSDWDQISEDFTGLRGFGDVVWYPVSSVPHLLSDGPQLFSEIGRE
                     KFRQQDARVAVRVTAEYVGAAPNAAVINGHYVPVPAPKVTPTASFPGVVTFSVPEQRL
                     GFGTLGLFLAPRTVHEANGMRILTSADDEPDVQAYQTAATMVQPLVEQWLGKLKQPLT
                     IFDLPEREDASFEQGGVTVTGLKALDPEKLTGTVCHGLAHAAFQSPREWLNEGVANFL
                     VTLWLEQSKGRTAALEYLESSRGALAIGEPASPGESAGEPLVTASDAIYYRSKATYVL
                     WMLRDLAGDKALSAALRGYDPAQDTTPEYFEHLVEQASGKDLKWFFDDWVYHDRGLPD
                     LSIAAVYPSATSQAGQYLVAIDLANDGYAAAEVPVTVHTRQGTQTERVQLPARSKVTR
                     RMLITGLPTEVVLNDGVVPEVQASIHKHEITITGSGN"
     gene            complement(6074..>6272)
                     /locus_tag="VFA02_04795"
     CDS             complement(6074..>6272)
                     /locus_tag="VFA02_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09198.1"
                     /translation="EASFAEPLSKTGYRLWNITYNDAGRIIAVSDDQHSYGEPVVLHP
                     SEPELKLKPVPPAPQPKPMPK"
BASE COUNT         1352 a         1601 c         1986 g         1333 t
ORIGIN      
        1 gaatgcggtg gaagtcccta tgatcggcga tggtcatctg caggccattg cgggaaaccg
       61 gaacaagtgg atgatccgct atctgccttt taaaggagaa gcgcgactac tgacggaagt
      121 cgagtcttca tgccagccga cggaagaacc ggtgagccat gatgtgacgc ttatctttac
      181 ctgcccacgg tctggaaatg accacctggt cagcgcgatc aatctggatg gaaaacttct
      241 gtggcaacag cgatgggacg cacgctacat ctggccgacg ttccagctca cgcagaacgg
      301 cagccgcttc gcctatagct cgttggaggt gtcgcgcccg gttggcccca tggatccggt
      361 ggaccaggat tcgattctga agcagatggt gggggtgttt gataccgcat caggcaaatt
      421 gcttctggtg aagaatgcca cgccgatact cagcgcggga cagaattatg cgctttctcc
      481 ggatggtatg cgttttgtta ttttgcgtga gggcgcgctt gaggtgtaca acctgccacc
      541 tatatctgag cattgagaac ggaacgtgcg gccacatcct caaagagatg cgggtgattg
      601 cagaatgatg ccaagcgggc cgcagctcgg ggtggcccag cattcattta taacttgaac
      661 aattcgcgca aacgtcttgt ttgggcgcga cttaccggta tctccgtctg cttcttatca
      721 tccatccgaa gctggtagct gctcttgaac cacggtacga cttccttgat gtggttgatg
      781 ttcaccacat aagagcgatg ggcgcgccag aagatggccg ggtcgagttg ttcgagcagt
      841 tcttcaagtg tgcggcattt ggattggcct tccagcgtgg gcgttgcgac tgtgatgacg
      901 ccttcgtcaa ttgaggcaaa gcagatgtct ttctggtcaa tcaagaagag gcggctgtgt
      961 gcctgaagga cgatttttcc gctgtgacgc tgcggttgtt gctgctgctc gatgagcttg
     1021 agcaaggcgc tgaggctggc gttgtcgccc aagtcgccga ttttttcgtg tccgtggacc
     1081 tcctgcaagc gctgacgggc gcgttctatg gcctgcagga cgcggtcacg gtcgaaaggc
     1141 ttgagcagat agtcaatggc atttacgtcg aatgccttga cggcgtattg atcgtaggcc
     1201 gtggcgaaga tgatctgcgg aaggtgctcg ctttcgccgt gttccagcaa ttttttcagg
     1261 acggcgaagc cgtccaggcc gggcatctgc acgtcgagaa atacgacgtc aggctggtgt
     1321 gtgcggatga ggtccacggc ctcgattcca ttggtcccct gcgccaggac ttcgacactg
     1381 ccggtggtgt cgaggaggta tttcagctct tcgcgggcga gctcttcgtc atcaataatg
     1441 agcgcagtca tggccatagg tggaacttaa agtataggaa ggactcgcca gccgaggcaa
     1501 acttccgcgc tgggcgtcaa tcctgaaaag cgtgcagacg cgcggggcga ttgtctgaag
     1561 caaggttatg tccgcagtgg acgcagtaga catcggtgat gcgcacgctg cggaagcact
     1621 ttccgcagga ggcggaaatc tgatacgcgc attgcgggca gaagtggtac tcactctgca
     1681 cccgtgttcc gcaggcaggg caaagcgaaa tgatgggctg gcgcaaaaga aaatacagca
     1741 cggcgccaat tcctccgggc atgacgaggc agatgatgat ccagatgcgc gcgctcatgc
     1801 cgcggcgcgg cgaatcgttg cttacgtaac ccaccatgag catgtaaaga gaagcgagaa
     1861 ggccccaggt gatggccagg tagagccgga tcccgatggg tggatgatgg tgcgcgccgc
     1921 gcgggaagac gacccagaag aggtactcca ttccgacgaa tgcgaggacc gcagcggcga
     1981 tagaccagcg cggaatcaac cttagttcat tggagaagga ggtttctgcc tcctgacgga
     2041 ttgggtagtt tgttcccatg ttgcattacc ggcggtcatg gcgcccgaag cgcaaaggcg
     2101 cctggtctct gaaactaatc aggagcacgc cgagtacggc agaaaaaaga accagggcca
     2161 ggaccgtaat catctccgac aatccaaaaa tctgttctcc gccaaagtaa tcgtcaatgg
     2221 tgctccagat ggccggcgta agcagcgcgg agagcgcaat gaagaccagg gacacaatgg
     2281 caatattgcg gcggttacgg cggcgctgct cgcgcaattc actggctgcc tgcattaccg
     2341 cgcggcgtgt gcgctgtact accggcgtac ttgcagaggt attcattccg gcaagcatgt
     2401 caatcaactg cggatccgga atgcgctgtg tgttcattgc gcctcctttc cgctggtcat
     2461 ggcttccaga cggggcttaa gcgacgcgag tccacgataa agccgggact tgacagtaga
     2521 caaaggcgcg cgggtaatct gggcaatctc ttcaagcgca agctcttcgt ggaagcggag
     2581 cacgaggact tcccggtgca gcgggtcgag cgtcaggagc aactcggcga cgatgcggct
     2641 gttttcgtag ctctggtaaa gctcgaacgg attcaggtcg ttgccgggta cttcaaacgg
     2701 gcggtcatct tcactggaat cgcagagttc ttccaggctg accatgctgc gcctgcgacg
     2761 gagatcaatc acaagattac gggcaatggt aaagagccac gtttcaaagc gggcattgcc
     2821 gttaaattgg tcgccgcgca tcagcacgcg catccaggtt tcctgaaaga gatcttcggc
     2881 gaggtcgcgg ttgccggtga ggtagagaag ataccgcagc agccggtgct gatattgcac
     2941 gatcagctca tcgagaagac cggcatcatg gcgcttgagt ccctgcgcaa tgagcgcgct
     3001 ctcttcgcgc gcctgcccgg tcagggtgat ggatgcggtg ctcattcctt tgtttgagac
     3061 ggaagaccca tcggcaagga cgcgcttctg caaaatattt tcgagctttt tgagaggact
     3121 catgagaaca gtagtgaatt gttggccgtc agttcgcagc tacatttact aaaacgataa
     3181 taggcccagc accagaacta ttcctttcat tatccggcag atagcggggg cgcggcaagc
     3241 gataaactga ggggatggaa gtcctttatg gcgtgcatcc tgttgaagaa gcgctgcggg
     3301 cgggaagccg ccgattcgat catgtttgct ttgcccggga gcgggctgga acgcatgtag
     3361 accagcggct gcagcgggta attgatgcgt gccgggaggc gggcgtccgg ttgcgctttg
     3421 agccgcgcga gcacctgacc cgtctggcga agacgcccag tcatcaggga gttgtagcgg
     3481 ttgtgcggga gaaggccaca ctggaactgg aagacctgct ggatgctcct gaggacggaa
     3541 gggccaggtt gtttcttgcg ctggacggcg tggaagatcc acagaatctg ggcgcgttgt
     3601 tgcggtcggc tgatggggcg ggcgcggatg gtgttgttct gacagagcgg cggtctgcac
     3661 cgctgagtcc ggtggccatc aaggcttcag ccggagcggc cgagcacgtc ccgattgcgc
     3721 gtgttgtgaa cctgagccgc gctctcgaac aaatgaaaga gcgcaacctg tggtgcgtgg
     3781 ggctggatga acgcgggacg atgtcttacg acgagttcga tttcacgtct aataccgtaa
     3841 ttgtgctggg gcgcgaggga gcaggcctgc atgatctggt aaagaggcat tgcgaccatc
     3901 tgctgcgcat tcccatggcg ggaaaggtgg cttcgctcaa tgtgtctgta gccggcgcgg
     3961 tggtgctcta tgaggcggca aggcaaagac gcgccggtca gatgaaggcg gcttctccgc
     4021 cgaagaagaa agagaaaaaa ggtctgggtt taagttgatg atgtttgcaa ggccgcttat
     4081 ggcggcattt ttgtgcggcg cggctgcctt tgcgcagcag gcggagcagg gccatggcaa
     4141 agtgctgttt tcgcgcaccg aggatgaagc gcagaaatcc actcccgaaa aaacgcagac
     4201 cattcaggca aaggcaacgg atgctgaacg ttctgcgatt accatcaccc gctatgatct
     4261 tgacgttcac ctgatgctga agcagcaggc aatggccgca caggcgcggg tgacgctccg
     4321 gaatgacggc aacgcaccgt tgacacatct gccgctgcaa gtatcgtctt cgcttcattt
     4381 cgagacgatt gctctgcatg gaaagaagct ccagtttgca cagaacctga tcaacagcga
     4441 tgcggaccat acagggcagc tccatgaagc cgtcattgaa ttgccggagg cgcttgcgcc
     4501 gaagtcggaa atcacgctgg acgcaggtta cagcggcgaa atcacggtct caacgaaacg
     4561 gctggagcag atcggcacac ctgccgatat tgcgaagcag tcagattggg accagatatc
     4621 ggaagacttc accgggctgc gcggctttgg cgatgtggtc tggtacccgg tgtcctcagt
     4681 tccgcacctg ttgagcgatg ggccgcagtt gttttccgaa atcggacgcg agaagttccg
     4741 gcaacaggat gcgcgtgttg ccgtgcgcgt tacggcggaa tacgttggcg cagcgccgaa
     4801 cgcagccgtt atcaacggac actatgttcc ggtacctgct ccaaaggtca cgccgacggc
     4861 atcgtttccc ggagttgtta ccttttccgt tcctgaacaa cgcctgggct ttggcacgct
     4921 gggcctgttt cttgcgcctc gcaccgtcca tgaagcgaac ggaatgcgca ttcttacctc
     4981 ggctgacgat gagccggatg tgcaggcgta tcaaacagcg gccacgatgg tgcagccgct
     5041 ggtcgagcag tggcttggca aattaaagca gccactgacc atcttcgatc tgccggaaag
     5101 ggaagatgca tcatttgagc aaggcggtgt tacggtgacg ggcctgaagg cgctcgatcc
     5161 ggaaaagctg acagggactg tatgccatgg ccttgcccat gcggcgtttc agtctccgcg
     5221 ggagtggctg aatgaaggtg tggcgaattt tcttgtgacg ctctggctgg agcagagtaa
     5281 aggccgcact gcggcactcg aatatctgga gtcttcacgc ggagcgctgg caatcggaga
     5341 gccggccagt ccgggggaaa gcgcgggcga gccgctggtt acagcatcag atgcaattta
     5401 ctaccgcagt aaggccacat atgttttgtg gatgttgcgc gatctcgccg gagacaaggc
     5461 gttgtctgct gcattgcgtg gatatgatcc ggcgcaggac acaacccctg aatatttcga
     5521 gcacctagta gagcaggcaa gcggcaagga cctgaaatgg ttttttgatg actgggtcta
     5581 ccatgaccgg ggtctgcctg acctctcgat agctgcggtc taccccagcg ccacttcgca
     5641 ggcaggacag tatctcgttg cgattgatct tgcgaacgat gggtatgcgg cagcggaagt
     5701 gccagtgacg gtgcacacgc gccaggggac acaaactgaa cgcgtgcagc ttcccgcgcg
     5761 ctccaaagtg acgcgccgca tgctcattac agggctgccg acggaggttg ttctgaatga
     5821 tggcgtagta ccggaagtac aggcgagcat tcataaacac gagataacca ttacggggag
     5881 tggaaattaa ggctgtttcc gtttcactgt ggtctacgaa gaggatggtt gtcattccgt
     5941 gcggagcgga aaatccaggc agttttggcc agggcctcgt gtcgctgaga ttcttcctcc
     6001 ctgcggttgt tgattgacgg acgcgacata tacgaaaaat gctgtaccgg cgagtgacaa
     6061 agcgaccggg aaatcacttt ggcatgggtt tgggttgagg ggccggggga accggtttca
     6121 gcttcagctc aggttcagaa ggatgcagta cgaccggctc gccgtatgag tgctgatcat
     6181 cgctgacggc gataatacgg cctgcatcgt tgtaggtgat gttccagagc ctgtagcccg
     6241 tttttgaaag cggctcggca aacgaggcct cc
//
LOCUS       DASRBV010000122         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_563965, whole genome shotgun sequence.
ACCESSION   DASRBV010000122 DASRBV010000000
VERSION     DASRBV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563965"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFA02_04800"
     CDS             complement(<1..1037)
                     /locus_tag="VFA02_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU09199.1"
                     /translation="MSEDKKEKPVALPKDGEPINLKQLAAILQLSQTTVSLVLNNSPA
                     AKSIPEHTRERVFQAARKYHYRPNYFARSLRKNRSMSIGVLAPDLSEGYFTMVMNGVE
                     EYLMRAQYFYFTASHYWDQDLMQEYPRLLVERAVDGFLLLNTPSEIRSPLPTVAISAH
                     NQSPGVTNIILDHKRAAELALKHLYDQGHRNIAFMKGPNIIPDTEYRWRSILEVARDL
                     GIKVHAEHCIQLEADSWSPGVGYGPMRDLLARTRDFTAIFCFNDISAIGVMRAIFDAG
                     LSVPGDISVIGFDDIMSAAFHKPSLTTVRQPLREMGQQGAQILLDMIAHPENNFPEEV
                     VMQPELIVREST"
     gene            complement(1088..2815)
                     /locus_tag="VFA02_04805"
     CDS             complement(1088..2815)
                     /locus_tag="VFA02_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZU09200.1"
                     /translation="MAPPSNPNTSEARKRWEEQTLNPALKKSPERPIGAASGINLDEN
                     GNARFTTISGVPVERLYTEDDLPPDWASQQDKYLGLPGHPPYTRGIHANGYRGKLWTM
                     RQFSGFASPEETNERYKYLLAQGGSGLSVAFDLPTLMGRDSDDPESEGEVGKCGVAID
                     SLDDMETLFSGINLEQTTVSMTINSPANVIWAMYLAVAEKQGASWKKISGTLQNDILK
                     EYIAQKEYIYPPAPSMRLVVDTFEFGAQHVPRFNPISISGYHIREAGSTAQQELAFTL
                     YDGIEYVEWALRRGLGIDDFAPRLSFFFNSHNDFFEEIAKFRAARKIWYRVMTGRFGA
                     KNPRSTWMRFHTQTAGVSLTAQQPKNNIARVAIQALAAVLGGTQSLHTDAYDEALALP
                     TEDAARIALRTQQILAYESGVADVVDPLGGSYFVERLTLDMEKAALTYFEKLDSMGGM
                     VRAIEHGFPQKEIAEASYAYQRAVEAGEKIIVGVNEYVMEEPQPQILYIDESVREAQT
                     AKLKRLRRERSNDAVARSLLALSHAAENEEENTMPCLLDCVKAYATVGEICNALKKVF
                     GTYEETSIA"
     gene            complement(2884..>3175)
                     /locus_tag="VFA02_04810"
     CDS             complement(2884..>3175)
                     /locus_tag="VFA02_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09201.1"
                     /translation="SGVGEEAKSFCRDYHLTKAEVRKMFRTYRELRPREMHDNYLYVP
                     CGIEGTVIVNGKTYTWRTQIGGTLDTTWPDGIEKTLGGEATDGLSEGEAGKK"
BASE COUNT          614 a          797 c         1000 g          764 t
ORIGIN      
        1 gtggattcgc gcacaatcag ttccggctgc atgacgactt cttcagggaa gttgttttca
       61 ggatgcgcga tcatgtcgag aaggatctgt gcgccttgct ggcccatttc acggagaggc
      121 tggcggaccg ttgtaaggct gggcttgtgg aaggccgcgc tcatgatgtc gtcgaagccg
      181 atgacggaaa tatcgccggg gacactgagt ccggcgtcaa aaatggcgcg catcacgccg
      241 atggcggaaa tgtcgttgaa gcaaaagatg gcggtaaagt cgcgcgtgcg ggcgagcagg
      301 tcgcgcatgg ggccatagcc tactccgggg gaccaactgt cggcttcaag ttgaatgcaa
      361 tgttcagcgt ggaccttgat gcccagatcg cgggcgactt ccagaatcga gcgccagcgg
      421 tactcagtgt ccggaatgat atttgggcct ttcatgaagg cgatgttgcg atggccctgg
      481 tcatacagat gcttcaacgc gagttcggcg gcgcgcttgt ggtcgagaat aatattggtg
      541 acgccagggg attgattatg cgccgaaatg gcgactgtag gcagcgggct gcgaatttca
      601 gacggcgtgt tcagcagaag gaagccatct acggcgcgct caaccagcag gcgcgggtac
      661 tcctgcatca ggtcctggtc ccagtaatgg ctggcggtga agtagaaata ctgtgcgcgc
      721 atgaggtatt cttcaacgcc gttcatgacc atagtgaaat agccttcgct gaggtcaggc
      781 gcgagcacac caatggacat gctgcggttc ttgcgcagcg aacgcgcgaa ataattcggg
      841 cgatagtgat attttctggc ggcctgaaag acgcgctcgc gtgtgtgctc agggatagat
      901 tttgcggcgg gagagttgtt caggaccagt gaaacggtgg tctgcgaaag ctgaagaatt
      961 gcggccagtt gcttgaggtt gatgggttcg ccgtctttgg gcaacgcgac tggcttttct
     1021 ttcttgtctt cactcatagg cgatttgtag cacgtttgga attgggaacg ctaccagata
     1081 attcggatca ggcgatgctg gtctcctcat aggtcccaaa cactttcttg agagcgttgc
     1141 atatttcacc gactgttgca taggccttca cgcaatcgag caggcagggc atggtgtttt
     1201 cttcctcatt ctcagcggca tgggacaaag caagcagtga gcgggcgacg gcatcgtttg
     1261 accgttcgcg gcgcagcctt ttgagctttg cggtctgggc ctcccggacg gattcgtcaa
     1321 tatagaggat ttgtggctgc ggttcctcca tgacgtattc atttacacct acgatgatct
     1381 tttctccggc ctcgacggcg cgctgatagg catagctggc ttcggcgatt tccttttgag
     1441 gaaagccgtg ctcgatggcg cgcaccattc cgcccatgct gtcgagcttt tcaaagtaag
     1501 tgagagcggc tttttccata tcgagcgtga ggcgctcgac gaaataggag cccccgagcg
     1561 ggtctaccac atcggccaca ccggattcat aggcgagaat ttgctgggtg cgcagggcga
     1621 tgcgggccgc gtcttctgtg ggcagggcaa gggcctcgtc gtaagcgtct gtgtggaggg
     1681 actgcgtgcc tccgagcaca gcggccagcg cctggatggc cacgcgcgcg atgttgttct
     1741 tcggctgctg tgcggtgaga gagacgcctg cggtttgcgt gtgaaagcgc atccaggtgg
     1801 agcgcggatt tttcgctccg aagcgcccgg tcatgacgcg ataccagatt ttccgcgcag
     1861 cacggaactt agcgatttct tcaaaaaagt cgttgtgcga gttgaagaag aagctcaggc
     1921 gtggagcgaa atcgtctatg ccaaggccgc ggcggagcgc ccattcgaca tactcgattc
     1981 cgtcatagag cgtgaatgcc aattcctgct gggcggtcga gccggcctcg cggatgtggt
     2041 atccggagat ggagatggga ttgaagcgcg gcacgtgttg cgcgccgaac tcgaaggtat
     2101 ctacaacaag gcgcatggag ggcgcgggcg ggtaaatgta ctccttctgt gcgatgtatt
     2161 ccttcaggat gtcattctgc agagtgccgg agattttctt ccagctggcg ccctgctttt
     2221 ctgcgaccgc gagatacatg gcccagatga cattcgcggg cgagttgatg gtcatggaga
     2281 cggtggtctg ctcgaggttg attccggaga agagcgtttc catgtcgtcg agcgaatcaa
     2341 tggcgacacc gcacttgccg acctcgcctt cgctttccgg gtcgtcggaa tcgcggccca
     2401 tcagcgtggg aaggtcgaat gcgacggaaa ggccgctgcc tccctgagca agcagatatt
     2461 tgtagcgctc gttggtttct tccggcgaag caaagccgga gaactggcgc atggtccaga
     2521 gcttcccgcg gtatccgttg gcatgaatgc cgcgcgtgta gggtgggtgt ccgggcaggc
     2581 cgaggtattt gtcctgctgc gaggcccagt ctggaggcag gtcgtcttcg gtatagaggc
     2641 gctcgacggg aacgccggag atggtggtga agcgagcgtt gccgttttca tcgagattga
     2701 tgccggaggc cgcgccaatg ggcctttccg gagatttttt caacgccgga ttcagcgttt
     2761 gttcttccca gcgctttctg gcttcggatg tgttcgggtt ggatggaggg gccatttgcg
     2821 aaactcctta gcataggtga taggagttat ccgaggcaaa ttcaggtgtc aggagtgttc
     2881 ctgttatttt ttccctgctt caccttcact aagaccgtca gttgcttcac cgcccagggt
     2941 cttttcaatc ccgtctggcc atgtggtgtc cagagtgccc cctatctggg tgcgccaggt
     3001 ataggttttt ccattcacga tgactgttcc ttctattcca caagggacgt aaaggtagtt
     3061 gtcgtgcatt tcgcgtggcc ttagttcccg gtacgtacga aacattttcc tgacttctgc
     3121 ttttgtcagg tgataatccc tgcaaaagct cttggcttct tcacctacac cagag
//
LOCUS       DASRBV010000123         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_107064, whole genome shotgun sequence.
ACCESSION   DASRBV010000123 DASRBV010000000
VERSION     DASRBV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107064"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFA02_04815"
     CDS             <1..504
                     /locus_tag="VFA02_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZU09202.1"
                     /translation="GEYEGIEDFKKKLKEHIANDKRRKLEAETKDKIIEALIAKFQFP
                     VPESLVQQQVDARLDRGLRALAAQGMRTEDMRKLNFERLREAQHDSAVNEVKGSLLLD
                     RIAEEEKVEVSDAEVDRELELFSLQSREPLESLRHRLTEDGGIARIREQIRREKTGNL
                     LYEKLTA"
     gene            577..1167
                     /gene="clpP"
                     /locus_tag="VFA02_04820"
     CDS             577..1167
                     /gene="clpP"
                     /locus_tag="VFA02_04820"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZU09203.1"
                     /translation="MALVPMVIEQTNRGERAYDIYSRLLRDNIIFLGTPIDDQVANLI
                     IAQMLFLSAEDPEKDIQLYINSPGGSITAGLAIYDTMQFIKNNVVTYCIGQAASMAAT
                     LLLAGTKGKRFALPNSRILIHQPLMGGLSGQATDIDIHAREIIRIREIINKLTAKHTG
                     QSLERIERDVERDFIMNAEQAKEYGIVDEIIDRPRT"
     gene            1208..>2026
                     /gene="clpX"
                     /locus_tag="VFA02_04825"
     CDS             1208..>2026
                     /gene="clpX"
                     /locus_tag="VFA02_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZU09204.1"
                     /translation="MKTRTGPDDALRCSFCHKSQDAVAKLISSPSDYPRAYICDECVA
                     VCNSILEDDRGETQQGAAPPHLPKPTEVRAFLDEYVIGQDQTKKKLAVAVYNHYKRIQ
                     MNRMRGNDVELTKSNILLVGPTGSGKTLLAHTLAKMLDVPFAIVDATTLTEAGYVGED
                     VENIILKLLQAADGDVARAQTGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKIL
                     EGTVANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLERVVGRRVGKKALGFK"
BASE COUNT          519 a          505 c          589 g          413 t
ORIGIN      
        1 ggtgagtacg aaggcatcga agatttcaag aagaaattga aggaacacat tgcaaacgat
       61 aagaggcgca agctcgaagc cgagacaaag gacaaaatta tcgaggcgct gattgcgaag
      121 ttccagttcc cggtgccgga atctttggtg caacaacagg tggatgcgcg tcttgaccgt
      181 ggactgcggg cgctggctgc acagggaatg cgaaccgaag acatgcgcaa gctgaatttt
      241 gagcggttgc gcgaggcgca gcacgattcg gccgtgaatg aagtgaaggg atcgctgctg
      301 ctggaccgta tcgcggagga ggagaaagtc gaggtctctg acgcggaagt agaccgcgag
      361 ctggagctgt tctcgctgca atcgcgtgaa cctctggaga gtctgcgcca ccgtttgact
      421 gaggacggtg gtatcgctag aatccgtgaa caaattcgca gggaaaaaac aggcaatctg
      481 ctttatgaaa agctgactgc ttaaaaagag gcgttggatt ggctggccgc tggcctttac
      541 gaatgggaat gactgtcccg gagtgagagt agaaatatgg cactggtacc aatggtgata
      601 gagcagacga accggggcga acgcgcctac gatatttact cccgtctgct gcgcgacaac
      661 atcatttttc tcggaacgcc cattgacgat caggtggcga acctgatcat tgcgcagatg
      721 ctcttccttt cagcagaaga cccggaaaag gacatccagt tgtacattaa ctctccaggc
      781 ggctcgatta ccgcgggact ggccatctat gacacaatgc aattcatcaa gaacaatgtt
      841 gtgacatact gcattggtca ggcggcgagc atggcagcga cgcttcttct tgccgggacc
      901 aaaggcaagc gttttgcgct tcccaactcg cgtattctga tccatcagcc tctgatgggc
      961 gggttgtccg gtcaggcaac ggacattgat atccacgcca gagaaatcat tcgtatccgg
     1021 gaaatcatca ataaattaac agcgaaacat accgggcagt ctctggagcg gatcgaacgc
     1081 gacgtcgagc gcgactttat catgaatgcc gagcaggcga aggaatacgg catcgtggac
     1141 gagattattg acaggccgcg aacatgagta tgagtaaaaa gtagtattct gtaggagcag
     1201 gtaacaaatg aaaacgcgca cgggacccga cgatgccctc cgctgctcgt tctgccataa
     1261 gtcgcaggac gccgtggcaa aactgatttc ctcgccaagc gactacccgc gtgcgtatat
     1321 ctgtgacgag tgcgtcgccg tatgcaactc cattctggaa gatgaccgtg gtgaaacgca
     1381 gcagggagca gccccgccac atctgcccaa gccaaccgag gtccgcgcgt ttctggatga
     1441 atacgtcatc gggcaggacc aaacgaagaa gaagctggcg gttgccgttt ataaccacta
     1501 caagcgcatt cagatgaacc ggatgcgcgg caatgatgtt gagcttacca agtccaacat
     1561 tctgcttgtt gggccgactg gaagtggcaa gacccttctg gcacacacac tggccaagat
     1621 gctggatgtt ccgtttgcga ttgtggacgc gactacactc accgaagcgg gatacgttgg
     1681 cgaagatgtt gagaacatca ttctgaagct tttgcaggcc gccgatggtg atgtggcccg
     1741 cgcccagacc ggaatcattt acattgatga aattgacaag attgggcgca aggacgaaaa
     1801 cccgtccatt acccgtgacg tctccggcga aggtgtgcag caggcacttc ttaaaatcct
     1861 tgaaggcacc gtagccaatg tgcctccgca gggaggacgc aagcatccgc accaggaatt
     1921 tactcctgta gacacaacca acatcctctt catctgcggc ggcgcgtttg taggactgga
     1981 gcgcgtggtt ggccgccgtg tggggaagaa ggccctgggt ttcaag
//
LOCUS       DASRBV010000124         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_310222, whole genome shotgun sequence.
ACCESSION   DASRBV010000124 DASRBV010000000
VERSION     DASRBV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310222"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /gene="thiC"
                     /locus_tag="VFA02_04830"
     CDS             complement(<1..1406)
                     /gene="thiC"
                     /locus_tag="VFA02_04830"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZU09205.1"
                     /translation="MSTNGISTPQSVTEILKPRAEWIAKRKAEAARTGDTNMSQMHFA
                     RKGIITEEMEFVARRENLPADLIRSEVAAGRMIIPANINHPELEPMAIGVASKCKVNA
                     NIGNSAITSNIDEELRKLHMAVHFGADTVMDLSTGGNIPEIREAILRHSPVPIGTVPI
                     YEALNRVKKLEDLNIDVYLEVIEEQAQQGVDYFTVHAGVLIQYVPLVAKRITGIVSRG
                     GAILAQWMTHHHKQNFLYENFDRIVKVMARYDVSFSLGDGLRPGSVADASDEAQFAEL
                     KTLGELTKAAWESDVQVMIEGPGHVPLDKIKEQVDKEVELCHGAPFYTLGPLVTDIAP
                     GYDHITSAIGAALIGWHGASMLCYVTPKEHLGLPNEKDVKDGMIAYKIAAHAADIARH
                     RPGARDRDDAISYARYTFDWEKQFALSIDPETARSMHDETLPDDYYKEAAFCSMCGPK
                     FCSMNWSSKVDEYNKTVHG"
     gene            1508..1975
                     /locus_tag="VFA02_04835"
     CDS             1508..1975
                     /locus_tag="VFA02_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZU09206.1"
                     /translation="MKSNQGTRISAAVVIVLGVIIYLAISGVQANKSYYVTIQELHGM
                     GDKAYTRHLRVAGFVQPGSIQRNGSDAQFVLVENNLTLPVTYRGDEPPPDTFKDNSQA
                     LAIGTFGRDGVFHATQLQAKCASKYAPAQPGQQSTGLTAQGGGMNTAPAQAAR"
     gene            2129..2329
                     /locus_tag="VFA02_04840"
     CDS             2129..2329
                     /locus_tag="VFA02_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09207.1"
                     /translation="MFVVSALDASLIPFPVPVSTDILVLLLAVAAGCSSRGRQRTGFI
                     INTFFGTVVLVTRLAMLGKVYF"
     gene            complement(2444..2962)
                     /locus_tag="VFA02_04845"
     CDS             complement(2444..2962)
                     /locus_tag="VFA02_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZU09208.1"
                     /translation="MKCALLTVCCIVLCSAAALAQEKKAAAGNAMSDQQFVDFAAQTD
                     MVEANLGQLAGTVASSQQVKDYAQMLVTDHTNDFDQLYKVAQQANLNRPNAIDAQHNK
                     AMIDPFQKLKGAAFDHRYVHEMIAGHTKALAVYKKEAAEAHDPALKSYAEQSLPVLQK
                     HLDGAKALEKAK"
BASE COUNT          627 a          830 c          937 g          768 t
ORIGIN      
        1 ccgtggacgg tcttgttgta ctcgtccact ttgctcgacc agttcatgga gcagaacttg
       61 gggccgcaca tggagcagaa ggctgcttcc ttgtagtaat cgtccggaag cgtctcgtcg
      121 tgcatggagc gggcggtttc cggatcaatg gagagcgcga actgcttttc ccaatcgaag
      181 gtgtagcggg cgtagctgat agcatcgtcg cggtcgcgcg cgccggggcg gtggcgggcg
      241 atgtcggcgg cgtgggcggc gattttgtag gcaatcatgc cgtctttcac atctttctca
      301 ttaggaaggc cgaggtgctc cttcggagtg acgtagcaga gcatggaggc gccatgccaa
      361 ccgatgagcg ccgccccgat ggccgaggtg atgtgatcgt atccgggggc gatgtcggtc
      421 acgagcggcc caagcgtgta gaaaggtgct ccgtggcaga gttcgacttc cttgtctacc
      481 tgctccttga ttttgtcgag cgggacgtgg ccggggcctt caatcatgac ctggacgtcg
      541 ctttcccagg cggctttggt cagctcgccg agtgtcttca attcggcaaa ctgggcctcg
      601 tctgaggcgt cggcgacaga gccggggcga agcccgtcgc ccagcgagaa gctgacgtcg
      661 tacctggcca tcaccttcac gatgcggtca aagttttcgt aaaggaagtt ctgtttgtgg
      721 tgatgggtca tccactgggc gagaatcgcg ccgccacgac tgacgattcc ggtgatgcgc
      781 tttgcgacca gcgggacgta ctggatcagc acaccggcat ggacggtgaa gtagtccacg
      841 ccctgctggg cctgctcttc gatgacttcg aggtaaacgt caatattcag gtcttcgagc
      901 tttttgacgc ggttgagggc ctcgtagatg gggaccgtac caatcggcac gggcgagtgg
      961 cgcaggatgg cttcgcggat ttccggaata ttgccaccgg tggagaggtc catcacggtg
     1021 tcagcaccga aatgcacagc catgtgcagc ttgcgcagct cttcgtcaat gtttgaagtg
     1081 atggcagagt tgccgatgtt ggcgttgacc ttgcatttgg aggcgacgcc aatggccatt
     1141 ggctccagct cggggtggtt gatattggca ggaatgatca tcctgcctgc ggccacctct
     1201 gagcggatga gatcggcggg aaggttttcg cgccgggcaa cgaactccat ctcttcggtg
     1261 atgatgcctt tgcgcgcaaa gtgcatttgc gacatgttgg tgtctccggt gcgcgcggcc
     1321 tcggctttgc gtttagcgat ccactccgcg cgtggcttca ggatttctgt gacagattgc
     1381 ggagtgctga tgccgttggt gctcataatc acctcgtatg aagtcgtttt gctgttagat
     1441 gtctgatgat gaatgcagga tgaatcctga ggggattata aattccggag taaaataaat
     1501 cacagtcatg aagagcaatc agggcacacg aatcagcgcc gcagtcgtaa tcgtactggg
     1561 cgtgatcatc tatcttgcca tcagcggagt gcaggccaac aagagctatt acgtcaccat
     1621 tcaggagctg cacgggatgg gagacaaggc ctatacgcga catctgcgcg tggccggatt
     1681 tgtgcagccc ggctcaatcc agcgcaacgg ttccgacgcc cagtttgttc tggtggaaaa
     1741 taatctgacc ctgcctgtga cttacagagg tgatgagcct ccgccggaca cattcaagga
     1801 caattcccag gcactggcga ttggcacctt cggacgcgat ggcgttttcc atgccacgca
     1861 gctccaggcc aaatgcgcgt caaagtatgc tcctgcgcag ccgggacagc agagcacagg
     1921 tcttacggcg cagggtggtg ggatgaatac ggcgcccgcc caagcggcgc gataatccag
     1981 gtctcaagcg gcacttttca atcccctgaa tctgcgcagt atggaagcct ggcggaggaa
     2041 actcgcgtcc tacagtgcgt gagtttttcc gagcttccgc gcaggcacca ttctccctca
     2101 ctggctgatt cagtacggtg cgctcggagt gtttgtggtc tcggccctgg acgcatctct
     2161 cattccgttt cctgtgccgg tgagcacgga catccttgtc cttctgctcg ctgtggctgc
     2221 tggttgcagc agccgtggga ggcagcgtac cggattcatc atcaatactt tcttcggcac
     2281 ggtggtattg gtgacgcgat tggcgatgtt gggcaaagtc tatttttgac tgaatgaaac
     2341 tgcttccaga gattttttat agacagtcac gtcagtcaac ggcctttgca atgcttctcg
     2401 tgcccttttt ccagaactgt gcggcgacgt tgattcctgg ctgctattta gctttttcaa
     2461 gcgctttggc gccatcgaga tgcttttgta gaacaggaag actctgttcc gcataggact
     2521 tcagggctgg gtcatgggct tctgccgctt ctttcttata gacggcgagt gctttggtgt
     2581 gtccggcgat catctcgtgg acatagcggt ggtcaaatgc agcgcctttc agtttctgga
     2641 aagggtcaat cattgcctta ttgtgttggg catcaattgc gttcggcctg ttcagattgg
     2701 cctgttgcgc aaccttgtat agctggtcga agtcgttcgt atgatcggtg accagcatct
     2761 gagcgtagtc cttgacttgc tgtgaggagg ccacagttcc tgccaactga ccaagattgg
     2821 cttccaccat atcggtttgt gcggcgaaat ccacaaactg ctgatcactc atcgcatttc
     2881 ccgccgcggc cttcttctcc tgggccaagg cagccgcaga acagagaacg atgcagcaaa
     2941 cggtcaaaag tgcacatttc atctgatttc ctccggtcga aaatacatct gccaaagtta
     3001 gatgctgatg tgcacggcaa aagtcaactt aacagatgat gctccatttc tacatcggca
     3061 acatcctgtg gtttgccttc aaaaaatgcg tacagacacg gcacaatcac cagcgtcagc
     3121 agcgtagatg tgaccagtcc gccaatcacc acgatggcca ga
//
LOCUS       DASRBV010000125         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_411592, whole genome shotgun sequence.
ACCESSION   DASRBV010000125 DASRBV010000000
VERSION     DASRBV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411592"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFA02_04850"
     CDS             complement(<1..806)
                     /locus_tag="VFA02_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZU09209.1"
                     /translation="MKKHNPPYHPQKTTHPKKHHPVKKPTPSPSSNATDPVFGQPQPS
                     PDPSSFKTPHPSDSGLYKKVNNSLVQPIPTARGGATEPVLTLEEVYGSQGTAKVGAIQ
                     QAGQIVFHSVGDTGSVIGPKTETLVADKMMSDFNEPNAADVPSFFFHLGDVVYSFGEA
                     KYYFDQFYEPYRNYQGPIVAIPGNHDGLVYTGDPAPSLNAYLRNFCADAPTHTSDAGG
                     LLRTAMIAPGVYFTFDAPFVRILGVYSNVLEDPGVISSEGGTRPLSDVQL"
     gene            1176..1721
                     /locus_tag="VFA02_04855"
     CDS             1176..1721
                     /locus_tag="VFA02_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09210.1"
                     /translation="MVPEDHALRQFFHDVVNDCYNEYLQMDDSEIASYVADLLTDFCS
                     SDRLYPIRDAEGRRLKDIGEMLLASDPVNGTAASFDVERRIRKHIGDYALFCTGMYPE
                     AVHKWRENENANLYELIRTGKESYYIVSQFDLFEYAEEAPLFARLSEAFESCMYGLNQ
                     VREELEKRMPGLPKKTPKFLM"
     gene            complement(1733..>3541)
                     /locus_tag="VFA02_04860"
     CDS             complement(1733..>3541)
                     /locus_tag="VFA02_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU09211.1"
                     /translation="FDWHRVFLMLKPGVDPATALGPLSQHLSAVNHAFEAGCSTCFRG
                     ATQESIARFLNQQLVIAPAGAGISDLQKDYRRFLGILGLMAALVLLIACVNIANMTTA
                     QAAARGQEMALRISIGAGRRRLVQLVLVQSALLALFAFGLGAVFAARSAPFVLSLINP
                     PEDPARLALSADWRVLFFGFGLILAVVLLLGLFPALRASAVRPVTALKGGEDPHLPRR
                     LMHGAIGLQVAFCFFVLFLSSLFLTSFKRLEHRPLGFSTDRLLLLETVADKGQPPVVW
                     SQTAEALRAVPGVDSVAISGWPLLGHIRINSDISIHGAPPSPTPAWFLNISPGWLATM
                     KIPLVSGRDFRPQDTSPGAAIINETFARTFFPGQDPIGLTFARGANQPLNTIVGVTPD
                     VPYSDLREPNRPVFYVPFNEMDGKSGMRTIDFSTFIVHTDTQDPLVLANSLRQLIAQR
                     HNGLRVSNVTTQLDLVRDQTIRERLIAMLAIFFAAVALLLAGIGLYAVLNYSVQQRRR
                     EIGIRMAVGAPRSRIVGLVTHDVFLTVVLGSCAGLALGFGAARYVESLFYQVKATDAE
                     MIALPACAILLTAVAAMFPAVLRALRTDPTEILRAE"
BASE COUNT          882 a          907 c         1076 g          676 t
ORIGIN      
        1 ttgagctgca catcgcttaa gggccgcgtg ccgccttcgc tggagatcac cccggggtct
       61 tcgagcacat tcgaatacac gccaagaata cgcacaaacg gcgcatcgaa ggtgaaatac
      121 actccgggcg caatcatcgc agtgcgcaga aggccgcctg catcactggt gtgcgtcgga
      181 gcatcagcgc agaagttccg cagataggca ttgagcgaag gagcaggatc gccggtatag
      241 accagaccat cgtgattgcc gggaatggcg acgataggac cttgataatt ccggtatggt
      301 tcgtagaact gatcgaaata atacttcgcc tcgccaaagc tgtagaccac gtcacccaga
      361 tggaagaaga acgagggcac gtcagctgca tttggttcat tgaagtccga catcattttg
      421 tcggcaacca gcgtttccgt ctttggccca atgacgctgc ccgtgtcccc cacagaatga
      481 aacacaattt gccccgcctg ctggattgcg ccgactttgg ccgtcccctg gcttccatag
      541 acttcctcaa gcgtgagcac aggctctgtg gcaccgccac gcgcagtggg aatgggctgc
      601 accagactgt tgttgacctt tttgtagagg ccggaatcgg acggatgggg cgttttgaac
      661 gacgaaggat cggggctggg ttgcggctgc ccgaacactg ggtcagtcgc attcgaagag
      721 ggcgacgggg ttggcttttt tactggatgg tgctttttcg gatgggtggt tttctgaggg
      781 tgatatggtg gattgtgctt tttcatggaa gtgcttctcg tattagatgc tcaaccctga
      841 agatacgccc attgccatag tgaaggtcat taagaaaacg tgacatgggc ctggaaaagc
      901 gccggtcccg gtcgtaaatc ctccatggat ttctgccatc ggtgaagcca gggaactttc
      961 ccctggactg gaacatttgc ggcaacctct cttcaagaag gctgcgcact ctgccgatga
     1021 aactgctaac aaagttttgg cggaatcaaa gaagtatctt gtgcttcgca acgaattgct
     1081 tcatcactaa tccataggag tgatgacttt tccggggaaa gcgggttgat aacagtagtt
     1141 gcttttccgg agcagcaggg aaggagaagt cagatatggt tcccgaagat catgcattgc
     1201 gccagttctt tcacgatgta gtgaacgatt gctataacga atatctccag atggacgaca
     1261 gtgagattgc gagctatgtg gctgatctgc tgactgattt ctgttcgtct gaccggcttt
     1321 atccgatccg tgacgctgaa ggccgccggc tgaaagatat aggcgagatg ctgcttgcat
     1381 ctgaccccgt gaatggaacg gctgcatctt ttgacgtaga gcggcgcatc cgcaaacaca
     1441 tcggcgatta cgcgctgttc tgcacgggaa tgtatccgga agctgtccat aaatggcgcg
     1501 agaacgaaaa tgccaacctg tatgagctga ttcgcacggg caaggagagc tattacattg
     1561 tttcgcagtt cgacctgttt gagtatgcgg aagaagcgcc gctgtttgca cgcttgtcag
     1621 aggcctttga aagctgcatg tacggactga accaggtacg cgaggaactc gaaaagagaa
     1681 tgccgggact tccaaagaaa accccgaagt ttctgatgta gacaccaaca gctcattctg
     1741 cgcgcagaat ttctgtcggg tctgtgcgaa gcgcgcgaag caccgccgga aacatagcag
     1801 caaccgcagt cagcagaatg gcgcatgcgg gcagagcgat catctccgcg tcggttgctt
     1861 ttacctgata gaagagcgac tcgacatagc gcgcggcccc aaatccgagc gcgagccccg
     1921 cgcagcttcc gagtacaacc gtaaggaaga cgtcgtgtgt gacgaggccc acaatccggc
     1981 ttcgcggagc gcccacagcc atgcggatac cgatctcgcg ccggcgctgc tgcacggaat
     2041 agttgaggac agcatagagc ccaatgcctg caagaagcag cgcaacagcc gcaaaaaaga
     2101 tagcgagcat ggcgatcaga cgttcgcgaa tggtctggtc gcgcaccaga tcaagctgcg
     2161 tagtgacatt tgaaacacga aggccgttgt ggcgctgcgc aataagctgg cggagtgagt
     2221 tggcaagcac aagcgggtcc tgggtatcgg tatgaacgat gaaagtggaa aaatcaatag
     2281 tcctcattcc ggacttgccg tccatttcgt tgaaaggaac atagaacacg gggcggttgg
     2341 gctcgcgcag atcgctgtaa gggacgtccg gggtaacgcc gacaatcgta ttgagtggtt
     2401 gattggcgcc gcgtgcgaaa gtaaggccga tggggtcctg accggggaag aaggtcctgg
     2461 caaaggtttc gttgataata gcagctccgg gagaagtgtc ctgggggcga aaatcgcggc
     2521 cggaaacgag gggaatcttc atcgtcgcca gccagcccgg agaaatattc agaaaccagg
     2581 caggagtcgg actcggaggc gcgccatgga tggagatgtc gctgttaatc cgaatgtgcc
     2641 ccagaagcgg ccagcccgag atggcaaccg aatcaacgcc aggaacagca cgcagcgcct
     2701 cggctgtctg gctccagacg acagggggct ggcccttatc ggcgaccgtt tcgagcagca
     2761 gcagccgatc ggtagaaaaa cccagtggac gatgttcaag gcgtttgaac gacgtgagaa
     2821 aaagcgaaga cagaaatagg acgaaaaaac agaaagcgac ctgaaggccg atggccccgt
     2881 gcataagtcg ccgtggcaaa tgcggatctt caccgccttt cagcgcggta acgggccgga
     2941 ctgcagaggc acgcagagca ggaaacaatc caagcagcag aacgacggca aggataagtc
     3001 cgaagccgaa gaaaagcacg cgccagtcgg cggaaagggc aagacgcgca gggtcctcag
     3061 gcggattgat gaggctgagt acaaatggcg ctgaccgcgc agcgaagaca gctcccaggc
     3121 cgaacgcgaa caaagcaagc agcgcgctct gaacgagaac aagctggacg agacggcggc
     3181 gtccggcgcc gatggagatt cgcagcgcca tctcctgccc gcgcgcggct gcctgggccg
     3241 tggtcatatt ggcaatattg acgcaggcaa tgagcaggac aagagcggcc atcaatccga
     3301 gaatgccgag gaaccgccga tagtccttct ggagatcaga gatacccgcg ccggctggag
     3361 cgatcacaag ctgttgattt aaaaagcggg caatggactc ctgggtcgcg ccgcggaaac
     3421 acgtagagca tccggcctca aaggcgtgat tgactgctga gaggtgctga ctaagcggcc
     3481 caagcgcagt ggccggatca acgcccggtt tcagcatcag gaaaacacgg tgccaatcga
     3541 a
//
LOCUS       DASRBV010000126        10155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_462131, whole genome shotgun sequence.
ACCESSION   DASRBV010000126 DASRBV010000000
VERSION     DASRBV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462131"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFA02_04865"
     CDS             complement(<1..687)
                     /locus_tag="VFA02_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZU09212.1"
                     /translation="MTKTKTHKVTLIPGDGIGPEVTDAVVRILEATGVKFDWERFAAG
                     AEAFEKYKEYIPKELYESIERNKLALKGPVTTPVGGGFPSINVTLRKKFELYANFRPI
                     RNLPGLETRYPNVDLIIIRENTEGEYVGLEHEVVPGVVESLKVITEKGSTRIARFAFE
                     YARKHGRKKIHAIHKANIMKMSDGLFLKCAREVGKGFPEITYGEHIIDNTCMQLVLNP
                     YQYDVLLLENL"
     gene            complement(801..1283)
                     /gene="nrdR"
                     /locus_tag="VFA02_04870"
     CDS             complement(801..1283)
                     /gene="nrdR"
                     /locus_tag="VFA02_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZU09213.1"
                     /translation="MKCPYCGFTQDRVVDSRESKEADSIRRRRECERCNKRFTTYERI
                     DEIPYMVVKKDGRRERFERQKVLSGLLHACEKRPVSASKLESLVDEAESFLMDSPERE
                     RTTTEIGELLMNKLKALDTVAYIRFASVYRDFKDVREFKEELEQLLSGKEAGKKGKGS
                     "
     gene            1490..3151
                     /locus_tag="VFA02_04875"
     CDS             1490..3151
                     /locus_tag="VFA02_04875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZU09214.1"
                     /translation="MADAKLQLIPLGGLGEFGMNCLVLRYKDDILVIDAGLMFPESEL
                     LGVDIVVPDITYLIENREHVRAIILTHGHEDHIGGLPWILSELNVPVYGTEFTLAYVE
                     GKLEEHKLLDQTELIEIIPGRKFSLGPFTIDPIRVTHSLVDCVALAIETPVGVVVHTG
                     DFKIDLSPPDGKAFDLHKFAEYGKRGVLALLQDSTNVDRPGYTPSEWAVKPRLDELFS
                     RTKKKLFFSCFSSSIYRIGIAVDLAHRHGRKVAIVGRSMVESADIAQDLGYLDVPPGV
                     IINPGQIADYAPEEVMVLISGTQGEPMSALSRAAVDNHKHARIQPGDTVVLSSRVIPG
                     NEKSIFRVIDHLCRRDAHVIYDDGASGLIHVSGHASQEEQRLMINLLRPKFFIPVHGD
                     YRHLKKHAELAQSMGVVDLALVIENGDVLELDHDDARTTGKVTAGRVCIDSGSTADVV
                     EDLVIRDRRHLSEDGIVLPILTINKLSGKVERMPEIVSRGFVGADPELLEAARKVVTQ
                     TLENSSDEEKADYGVIKEKIRIDLKRYIQKNTSRRPLIMPVILEI"
     gene            3155..4120
                     /locus_tag="VFA02_04880"
     CDS             3155..4120
                     /locus_tag="VFA02_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZU09215.1"
                     /translation="MLVSLEEFRAAQKRIQGVAMHTPLVRLQISGGAENVFMKAEGLQ
                     PVGSFKLRGAYNKIAQLSAEERRRGVITYSSGNHAQGVAYAARALGTKAVIVMPSNAP
                     EVKKRATAALGAEIVEVGPSSSERKQKAEDLEAKFGYVMVPPYDDRAIIAGQGTCGLE
                     ILEDLPDVDLVLAPVSGGGLLSGVATAIKLIKPDVRVLGIEPELAADAQESFRTGRLV
                     TWPAELTTRTAADGLRTQSMGDLNFEHIRAYVDDIVTVKEEEIAEAMRVLLYQARLTP
                     EPSGAVAAAAVLFHREALLPFRNCVSIMSGGNVEPDVLRRLLAMG"
     gene            complement(4136..4528)
                     /locus_tag="VFA02_04885"
     CDS             complement(4136..4528)
                     /locus_tag="VFA02_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU09216.1"
                     /translation="MLPLLFGPLAWADHDQPVPSFAKPFDQLPVHANGANISRPILNG
                     MTHSGTHLEVHETTLAPGAMPHPPHHHVHEELFLLSKGTVEVTIAGKSTALGPGSAAF
                     VHSGEEHGVRNTGQEPAQYFVVAIGQES"
     gene            4648..5811
                     /locus_tag="VFA02_04890"
     CDS             4648..5811
                     /locus_tag="VFA02_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZU09217.1"
                     /translation="MDLSAIQSALREQKLDAWLFYDHHHRDPIAERILGLDPHAHITR
                     RWYYVVPAQGEPHKLVHRIEQGRLDVLPGGKTQYSSWQELQSGLEMMLAPYLRIAMQY
                     SPRNAIMYVSMVDAGTIEFLREIGKTIISSADLVSQFEAVLSQEQVASHYEAQRALDE
                     ILAEGFRRIGERSSSGITEYEMKTWFLEAIERAGMITENGPNVSVNENSSDSHYEPTA
                     ERSRKIRKGDFLLIDIWSRINRPGSCWYDITWTGVVGRPPSEREQQVFTTVRNARDAA
                     IRAVKDAFAKKQPIAGWQADDAARAVIKEAGFAQYFTHRTGHNIATELHGNGAHLDNL
                     ETHDERRILPFTCFSVEPGIYLPEFGVRSEVNMMTTRDKAEVTGRIQQDLVQI"
     gene            complement(5831..7384)
                     /gene="nadB"
                     /locus_tag="VFA02_04895"
     CDS             complement(5831..7384)
                     /gene="nadB"
                     /locus_tag="VFA02_04895"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZU09218.1"
                     /translation="MADFVVVGAGIAGLRAAIGLAEAGHVLVITKEQLGESNTQYAQG
                     GIAVALGGEEDVSLHLEDTVNAGDGLVNREAAKVLVQEGPRRVEELLAWGTRFDRENG
                     KLMFTREGAHSRNRILHAHGDATGAEIGRSLLAHARSIKSITLMEWTMCVDLLVEGDE
                     VLGLTVLDRSGNLQTICARAVLLASGGAGQVYSDTTNPAVASGDGIAMAYRAGAEISD
                     MEFYQFHPTALGLPGVPRFLLSEALRGEGAYLRNAKGERFMERYHPLLELAPRDVVAR
                     AITQEGIGPNRETLPVYLDMRHVKGIDLHQRFPGISGFLSKYGLELGRDLIPVRPAAH
                     YLMGGVRTDVHGRASLKRLYAAGEAACTGVHGANRLASNSLLEGLVFGARAAEAMSEE
                     PDPAAPEILDVPLPAGKLTNTGDLLQRLQQTMWQKAGLLRDAAGLNDAVSVLRQIQEQ
                     TPEGKNRASLELRNLHTVAELIVKSALAREESRGAHYRNDFPQKDDSHFQKHSIVSRA
                     RDSVEFSLF"
     gene            7426..8511
                     /gene="aroB"
                     /locus_tag="VFA02_04900"
     CDS             7426..8511
                     /gene="aroB"
                     /locus_tag="VFA02_04900"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZU09219.1"
                     /translation="MHTIQVKTASADYPVHVGSGLLANLYEKLPKQKRVFVLTSPEIW
                     GLWAERFLASFPKNEQPVVLFLPPGEQHKRMAQVERLATELSSAGADRSSLLIAFGGG
                     IIGDLGGFLAAIYMRGIDYVQVPSTLLAQVDSSVGGKTGVNLATGKNLIGSFHHPLAV
                     FADIDLLQTLPDRELRAGLFESIKAGIIRDEQLFEFMERNVNAILGRQTEALEHVIAA
                     SIRMKAEVVVIDERESGLRMILNFGHTIGHAIEAVTRYKKLLHGEAIAWGMIAALHVA
                     EARKTITPEQALRMNRTIHLYGPLPSFKTSAEKLLEAAGRDKKNRLGVRRFILPVGIG
                     DAKVVEDVTNQELLSASEWMLKQARKA"
     gene            8511..9251
                     /locus_tag="VFA02_04905"
     CDS             8511..9251
                     /locus_tag="VFA02_04905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HZU09220.1"
                     /translation="MPAQGAQPAGATDTSSAASAVREMFDSIAPRYDLLNHVLSCNID
                     RLWWRRTARAFRDVLSRPDAAVLDICCGTGDMTLALLRLRPEKAAPVLAADFSHQMLV
                     RGKAKLAGHNAVVLEADALHLPLPDGSVDLITTAFGFRNLANYRAGLEEFHRVLKPGG
                     QLGILDFSEPGGLVGKLYAFYFRRVLPAIGSRISGVSGPYAYLPASVHRFPPPAEMLA
                     LMHESGFTGSSWEPYSFGIAGLYRGMKP"
     gene            9257..>10155
                     /locus_tag="VFA02_04910"
     CDS             9257..>10155
                     /locus_tag="VFA02_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZU09221.1"
                     /translation="MQAATAHASHRAGAEKRAVAIVSVMAATLMTLLKLVTGVITGSL
                     GMLSDAAHSGIDLAGAGLTFLSVRVSDRPADENHPYGHGKIENVSAFVETFLMLASSV
                     WITYEAVARIFFHPVEIRYSFWPLLVLALSMSVDYWRSKQLMAVANRYGSQALEADAL
                     HFRSDIWSSIAVFAGLSASWLGSHLGFPALRIADPIAAICVSVLILYFGWKLARRTVA
                     ELVDTIPPETRRRVLYELRHTNGVLAVDRARMRRSGNSYFADFTLSLPRQMTFQRSEQ
                     LVKDATSAVRRVLPDADVVIHTVP"
BASE COUNT         2144 a         2913 c         2871 g         2227 t
ORIGIN      
        1 gaggttttca agcagcagca catcatactg gtatgggttc agcaccaatt gcatacaggt
       61 gttgtcaatg atgtgctcgc cataggtgat ctcgggaaag cccttgccca cttcgcgcgc
      121 gcacttcaga aacaggccat cggacatctt cataatattg gccttgtgga tggcgtggat
      181 cttcttgcgt ccatgtttgc gcgcatattc aaaggcaaat ctggcaatgc gcgttgagcc
      241 tttttccgtg atgaccttca gagattcgac aacgcccggg accacttcgt gctccaggcc
      301 gacgtactcg ccttcagtat tttcacggat gatgatgagg tccacattgg gatagcgcgt
      361 ttcaagacca ggaaggttcc tgataggacg gaagttcgca tatagctcaa acttcttgcg
      421 cagggtcacg ttgatggatg gaaacccgcc gcccaccgga gtggtcaccg ggcctttcag
      481 cgccagcttg ttccgttcaa tggattcgta aagctccttt gggatgtact ccttatattt
      541 ctcaaaggct tcagctcccg ctgcaaaacg ttcccagtcg aacttcaccc ctgtggcttc
      601 cagaatgcgg accaccgcat cggttacctc cgggccaatt ccatcgccgg gaatgagcgt
      661 tactttgtgt gtttttgttt tggtcataat ctttcagccg cgccacaaaa cggcgcatgt
      721 cagcaagtca gccagtcggc gctgtcagcg aaaaagcgga tagcggcgct ttgcgcctgt
      781 cagccttcgc cgaccgccgt ttagctgcct ttccccttct ttcccgcttc cttcccgctc
      841 aacaactgtt ccagttcttc cttaaattcg cgcacgtcct taaaatcgcg gtaaacactg
      901 gcaaagcgga tatacgccac ggtatcgagc gccttgagtt tgttcatcag cagctcgccg
      961 atttccgtcg tggtgcgttc gcgctcgggc gaatccatca ggaagctctc ggcctcgtct
     1021 accagtgatt ccagcttgct ggccgaaacc ggacgcttct cgcaggcgtg cagcagaccg
     1081 ctcaacacct tttgccgctc aaagcgctcc cggcggccgt cctttttcac caccatgtat
     1141 gggatttcgt ctatgcgttc atacgtggta aaacgcttgt tgcagcgctc acactcgcgg
     1201 cggcggcgga tggaatcagc ctctttgctc tcgcgcgagt ccaccacccg gtcttgcgta
     1261 aacccgcagt aaggacattt catcttgtct gtctttcttc attctaacaa ggagatgaag
     1321 tttgctttgt ccttcccagg cctgccttca cgctcctgat gagagattgc tcatgaaacg
     1381 gtatggctct ccctatcagt gccggtccat ttctatggtt tactaagaga agaacccgtc
     1441 ctcaaagaac tcatgcgcca gaggcgcgtg cgcctggacg gcaagtccaa tggctgacgc
     1501 aaaactgcaa ttgatcccgc tgggcgggct gggggagttc ggtatgaact gcctcgtcct
     1561 ccgctataaa gacgacattc tcgtgattga cgccgggctg atgttccctg aatcggaact
     1621 gcttggcgtg gacattgtcg tccccgacat cacctatctg atcgaaaacc gtgaacacgt
     1681 acgcgccatt attctgacgc acggacacga agaccacatc ggcggactgc cctggattct
     1741 ctccgagctg aatgttccgg tctatggcac cgagttcacg ctggcatatg tggaaggcaa
     1801 actcgaagag cacaaactgc tcgaccagac ggagctgatt gagatcattc cgggcagaaa
     1861 gttctcgctc ggtccgttta cgattgatcc catccgcgtt acccacagtc ttgtggattg
     1921 cgtggccctg gccattgaga ctccggtcgg cgtggtggtg catacgggtg atttcaagat
     1981 tgacctctcc ccaccggacg gcaaggcctt tgacctgcac aagtttgccg agtatggcaa
     2041 gcgtggcgtg ctggccctgt tgcaggactc aaccaacgtg gaccgtcccg gctatactcc
     2101 cagcgagtgg gcggtaaagc cccgtctgga tgaactgttt tcccgcacga aaaagaagct
     2161 cttcttcagt tgcttttcgt cgtctattta ccgtattggc attgcggtag acctggcgca
     2221 ccggcacggc cgcaaggtag cgattgtcgg ccggtcaatg gttgagtcgg cggacattgc
     2281 gcaggacctc gggtatctgg acgtgccgcc cggagtcatc atcaaccccg gtcagattgc
     2341 ggactatgcg ccagaagagg tcatggtcct tatcagcggg acgcagggcg agccgatgag
     2401 cgccctgagc cgcgccgccg tggacaatca taagcacgcg cgcatccagc ccggagacac
     2461 tgtcgtcctc agctcgcgcg tcatccccgg caatgaaaag agcatctttc gcgtcattga
     2521 ccacctctgc cgccgtgacg ctcacgtgat ttacgatgac ggcgcatccg gcctcatcca
     2581 cgtcagcggg cacgccagcc aggaagagca gcgactgatg atcaacctgc tgcggccaaa
     2641 gttcttcatc cctgtgcacg gcgattatcg ccacctgaaa aaacacgccg aattggccca
     2701 gagcatgggt gttgttgatc tggcgctggt cattgagaac ggtgatgttc tcgaactgga
     2761 ccatgacgat gcgcgcacaa ccggcaaagt caccgccggc cgcgtctgca ttgattccgg
     2821 ttcgacggca gatgtggtgg aagatctcgt catccgcgac cgccgacatc tgagcgagga
     2881 cggcattgtg ctgcccatcc tgactatcaa taagctcagc ggcaaggtcg aaagaatgcc
     2941 ggaaattgta agccggggct ttgtgggcgc cgatccggaa ttgttagagg ctgcccgcaa
     3001 ggtcgttacg cagacgctcg aaaactccag cgatgaagaa aaagcggact acggagtcat
     3061 caaagaaaag atccggattg atctgaagcg gtacatccag aagaacacca gccgcaggcc
     3121 attgatcatg ccagtgattc ttgagatata agccatgctc gtgtctctcg aagagttccg
     3181 cgctgcccaa aagcgcattc agggcgtcgc catgcatacg cccctagtgc ggctgcaaat
     3241 ttccggggga gcggaaaatg tgttcatgaa agccgagggg ctccagcctg ttggcagttt
     3301 caagctgcgc ggcgcataca acaagatcgc gcagttaagt gcagaagagc gcaggcgcgg
     3361 ggtcattacc tattcgagcg gcaatcatgc gcagggagtg gcatatgcgg cacgggcgct
     3421 ggggacaaaa gcagtaatcg taatgccatc gaatgctcca gaagtgaaaa agcgcgcaac
     3481 ggcagcatta ggggccgaaa ttgtcgaagt aggcccttcc agctcagagc ggaagcagaa
     3541 agccgaagat ctggaggcga agtttggata tgtgatggtg cctccttatg atgaccgcgc
     3601 catcatcgcc ggacaaggca cctgcggtct tgaaattctt gaagacctgc cagacgtgga
     3661 ccttgtcctc gctcctgtca gcggcggagg actgctcagc ggcgtggcca cagctatcaa
     3721 gttgatcaaa cccgatgtgc gcgtcttggg cattgaaccc gaattggccg ccgatgcgca
     3781 ggagagcttc cgcactggga ggctggtcac gtggccagca gaactgacca cacgcactgc
     3841 cgcggacggc ctgcgcacgc aaagcatggg tgatttgaat ttcgaacaca tacgcgctta
     3901 tgtagatgac atcgtcacgg tgaaagaaga agaaatcgcc gaagccatgc gtgtgttgct
     3961 gtatcaggca cgtctgacgc cagaaccaag cggcgccgta gcagcagcag cggtactctt
     4021 ccaccgggaa gcattgctcc cgttccgcaa ttgcgtctcg attatgagcg gcgggaatgt
     4081 cgaaccggac gtattaaggc ggctattggc aatgggctaa gaagcgttgg gaatgtcagc
     4141 tctcctgccc aatcgcaact acgaaatact gcgccggttc ctgcccggtg ttgcgaacac
     4201 catgctcttc cccggaatga acaaaggccg ctgatcccgg tccgagggcc gtgctcttgc
     4261 ctgcaatcgt gacctcgacc gttcctttgg aaagaagaaa cagttcttca tgcacatgat
     4321 gatgcggcgg atggggcatc gcaccaggag ccagtgttgt ctcatgcact tcaagatgcg
     4381 tgcctgagtg cgtcatgcca ttcagaatcg gccgcgagat gtttgcccca ttcgcgtgga
     4441 cgggcaattg atcaaagggt ttggcgaatg atgggaccgg ctgatcatgg tctgcccatg
     4501 ccagcggacc gaaaagcagg ggaagcatct gacaaagccc ccgtcgtgaa atctccatag
     4561 ccacctctca agtgtctgcg cactgattgt aatggagcaa ggtccggcca tgacgcacag
     4621 tttcccgctc cggtacactc tttccttatg gacttgtctg cgattcaatc ggcactccgc
     4681 gagcagaagc tcgatgcatg gcttttttac gatcatcatc accgcgaccc tatcgccgaa
     4741 cgcatcctcg gcctggaccc gcacgcgcac atcacgcggc gctggtatta cgtggttccc
     4801 gcgcagggag aaccgcataa gctggtgcat cgcattgaac aggggcggct tgacgtcctg
     4861 cccggaggca aaacgcagta ttcctcatgg caggaactgc aatccggcct ggaaatgatg
     4921 cttgcgcctt atctgcgcat cgccatgcag tactcaccgc gcaacgcgat catgtatgtc
     4981 tcaatggtgg acgcagggac cattgaattt ctacgtgaaa tcggcaaaac catcatcagc
     5041 tctgccgatc tggtcagcca gtttgaggcc gtgctttcac aggaacaggt ggccagccac
     5101 tatgaggccc agcgcgccct ggatgagatt ctggcggaag gctttcgcag aatcggcgag
     5161 cgctcctcat ccggaattac ggaatacgag atgaagacat ggtttctgga agcgattgaa
     5221 cgcgcgggaa tgatcacgga aaacggcccg aatgtctcag tgaatgaaaa ttcttcagat
     5281 tcacattacg agcccacggc agagcgctca cgcaaaatcc ggaagggcga ctttcttctg
     5341 attgatatct ggtcgcgcat aaaccgcccg ggatcctgct ggtatgacat tacatggacc
     5401 ggagtggtag gccgcccgcc ctctgagcgc gaacagcagg tctttacgac ggtcaggaat
     5461 gcgcgggacg ctgctattcg cgccgtgaag gatgcctttg caaagaagca gcccattgcc
     5521 ggatggcagg ccgatgatgc tgcccgcgcc gtcatcaaag aagcaggctt tgcgcagtac
     5581 tttacccacc ggaccggaca caatatcgca accgagcttc acggcaatgg cgcgcacctc
     5641 gataatcttg agacacacga tgagcggcgc attcttccat ttacctgctt ttccgtggag
     5701 ccgggcattt atcttccgga atttggcgtg cgcagcgaag tgaacatgat gaccacgcgc
     5761 gacaaggccg aagtcaccgg acgtatccaa caggacctgg ttcagattta acgccgcggg
     5821 cccgggcgaa ttaaaaaagc gagaactcca ccgaatccct ggccctgctc acaatggaat
     5881 gtttctgaaa atgcgaatca tccttttggg gaaagtcgtt gcgatagtgc gcgccgcggc
     5941 tctcttcccg cgccagagct gacttcacaa tcagctccgc cactgtgtgc agattgcgca
     6001 actccagaga tgcgcgattc tttccctctg gcgtctgctc ctggatttgc ctgagcacgc
     6061 tcacggcgtc gttcaatccg gcggcatcgc gcaggaggcc cgctttttgc cacatggtct
     6121 gctgtaatcg ctgcaaaaga tctccggtgt tggtcaactt cccggcgggc agcggaacat
     6181 cgaggatttc aggagcagcc gggtccggtt cttcgctcat cgcttctgct gcccgcgcgc
     6241 cgaagacaag cccttcgaga agcgagttgc tcgccagacg gtttgcgccg tggacaccgg
     6301 tgcatgccgc ttctcctgcg gcgtagaggc gcttcagcga agccctgccg tggacatcgg
     6361 tcctgacccc gcccatcaga tagtgcgccg caggacgcac aggaatgagg tcgcggccca
     6421 gctcgagtcc gtattttgac aggaaccctg aaataccggg gaagcgctga tgcaggtcta
     6481 ttcccttgac gtgccgcatg tcgaggtaga cgggtaaagt ttcgcgattg gggccaattc
     6541 cctcttgcgt aatggcgcgt gccaccacgt ctctcggagc aagctcgagc aatggatgat
     6601 accgctccat aaagcgctcg ccctttgcat tgcgcagata tgccccttcg ccgcgcaagg
     6661 cttctgaaag cagaaagcgc gggacgccgg gcaggcccaa cgccgttgga tgaaactgat
     6721 aaaactccat atcagagatt tcagcgccgg cgcggtatgc catggcgatg ccgtccccac
     6781 tggccaccgc agggttcgtc gtatcactgt agacctgacc tgcgcctccg ctggccagca
     6841 gaacggcacg cgcgcagatg gtttgcagat taccgctgcg atcgagcacg gtcaggccga
     6901 ggacttcatc gccctccacc aaaagatcca cgcacatcgt ccattccata agagtgatgc
     6961 ttttgatgga gcgggcatgt gcaagcagtg agcggccaat ctcagcaccg gtagcgtctc
     7021 catgcgcgtg caggatacgg tttcggctgt gcgcgccctc gcgcgtgaac atcagcttgc
     7081 cattttcgcg gtcaaaacgc gtaccccagg caagcagttc ttctacgcgg cgcggtcctt
     7141 cctgaacaag cacctttgcg gcctcacgat tcacaaggcc atcgcctgca tttacggtgt
     7201 cttcaagatg cagcgagacg tcctcttcgc cacccagggc gacagcgatg ccgccctggg
     7261 catactgcgt gtttgattcg ccaagctgct ctttggtaat gaccaggacg tgtccggctt
     7321 cggcgagtcc aatggccgcg cgcagaccgg caatgcctgc gcccacaaca acgaaatctg
     7381 ccataaaatt tgaggctatc atgactgcga tacgatgaaa gaagcgtgca tacgattcag
     7441 gtcaaaacag cttctgcgga ttatcccgta catgttggca gcggactgct tgccaattta
     7501 tacgagaaat tgccaaagca aaaacgcgtc tttgtcctga cctcgccgga gatatggggg
     7561 ctgtgggcgg agcgctttct tgcatctttc ccaaagaatg aacaaccagt cgtgctgttt
     7621 ctgccgccgg gagagcagca caagcggatg gcgcaggtag agcggctggc gacggagctt
     7681 tcttccgcag gggcagaccg cagctcgctg ctgattgcct tcggcggcgg cattatcggt
     7741 gacctcggcg gatttctcgc tgccatctac atgcgcggca ttgactatgt tcaggttcca
     7801 tctaccctgc tggctcaggt agattcgtcg gtgggcggaa agacgggggt gaatcttgca
     7861 acaggcaaaa accttatcgg cagctttcac cacccgctcg cagtctttgc ggacattgac
     7921 ctgttgcaga ccctgccgga ccgcgagctg cgcgccggac tcttcgagag catcaaagcg
     7981 ggcatcattc gtgatgagca gctctttgaa tttatggagc ggaatgtcaa cgccattctt
     8041 ggccggcaga cagaggccct cgagcatgtg attgccgcct ccatccgcat gaaggccgaa
     8101 gtggtggtca tagatgagcg cgagtcgggt ttgcgtatga ttctgaactt tggccatacg
     8161 attggacatg cgatcgaggc tgtcacacga tacaaaaagc tgctgcacgg cgaagccatt
     8221 gcctggggca tgattgcggc gctgcatgtg gccgaggcgc gcaaaaccat tacgccggaa
     8281 caggcactgc gcatgaacag gaccattcat ctgtatggcc cgctgccatc gttcaaaacc
     8341 agcgcagaaa aactgctgga agcggcgggg cgcgacaaaa agaaccgctt gggcgtgcga
     8401 cggttcatcc ttccggttgg catcggtgat gcgaaggtcg tggaagacgt gacaaaccag
     8461 gaacttttgt ctgcatcgga atggatgctg aaacaggcgc gcaaagcata atgccagcac
     8521 aaggagcaca acccgccggg gccaccgaca catccagcgc agccagcgcc gtacgcgaaa
     8581 tgttcgacag cattgcgccg cgttacgacc tgctcaacca tgttctttcc tgcaatattg
     8641 accgtttgtg gtggcggcga acggcgaggg cgtttcgcga tgttctgtcc cgtcctgatg
     8701 ctgccgttct cgatatctgc tgcgggaccg gagacatgac gctcgcgctg ctgcggctgc
     8761 gtccggaaaa agcagcccct gtgctggccg cagatttttc tcaccagatg ctcgtgcgcg
     8821 gaaaggcaaa gctcgctggc cataacgctg ttgttctgga ggccgatgcg ttgcacctgc
     8881 cattgccgga cggctcggtt gacctgatta ccactgcatt tggctttcgc aatctggcga
     8941 attatcgtgc cgggctggaa gagttccatc gtgtactgaa gccaggcggg caattgggca
     9001 ttctggattt cagcgaaccg ggagggcttg tgggcaaact ttatgctttc tatttccgcc
     9061 gcgttcttcc ggcgattgga tcccgcatct cgggagtttc cggaccttac gcctatctgc
     9121 ctgcgtcggt ccaccgcttt cctcctccgg cggaaatgtt ggcgctgatg cacgaaagcg
     9181 gatttacggg gagttcatgg gagccttaca gctttggcat tgcgggcctc taccgcggta
     9241 tgaaacccta agaacaatgc aggctgctac agcccatgcc agccatcgag caggcgctga
     9301 aaagcgcgcg gtcgccatcg tgtctgtcat ggcagcgact ttaatgacgc tgctgaagct
     9361 cgtgacgggg gtcattaccg gaagtctcgg tatgctctcc gatgcggcgc attccggcat
     9421 tgatcttgcg ggcgcggggc ttacgtttct ttcggtccgc gtgtcagaca ggcccgcgga
     9481 cgaaaaccat ccctatgggc acggcaagat cgagaatgtc tcggcatttg tcgagacttt
     9541 tctcatgctg gcttcgagtg tctggatcac ttacgaagct gttgcccgca tcttctttca
     9601 tccggttgag attcgctact ctttctggcc actactggta ctggcgctct ctatgtcagt
     9661 agattactgg cgatcgaagc agttaatggc ggtcgccaat cgttatggaa gccaggcgct
     9721 tgaggctgac gcgctgcatt tccggtcaga catctggtcc agcattgccg tctttgccgg
     9781 actttctgct tcatggcttg gttctcatct cggctttccc gcactgagga ttgccgaccc
     9841 aattgctgct atctgcgtct ctgtgttgat cctctacttt ggatggaagc tggctcgacg
     9901 taccgtggcc gagttggtag acaccatccc tccggaaaca cggcgccgcg tgctttacga
     9961 gcttcggcat acgaacggcg tgcttgcagt agatcgagcg aggatgcgcc gttccggcaa
    10021 ctcatatttc gcggacttca cgctctctct gccgaggcag atgacgttcc aacgctcgga
    10081 acaactggta aaagacgcga cctctgccgt ccgccgtgtt ttgccggatg ccgatgtagt
    10141 catccataca gtccc
//
LOCUS       DASRBV010000127         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_411695, whole genome shotgun sequence.
ACCESSION   DASRBV010000127 DASRBV010000000
VERSION     DASRBV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411695"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFA02_04915"
     CDS             complement(<1..974)
                     /locus_tag="VFA02_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HZU09222.1"
                     /translation="MNSAVVKAASLFAVAVLASLVCACGPVTGRPAASASPPQIPLSA
                     SDPLANYALTGNVQPVHDPSIIRQGDTYYAFTSDMLALVPGNYLPIRCSQDKVNWSSC
                     GSVFPKQMPAWTTGAVPGTLCLWAPDVSYFHGLYHVYYAASTAGSQLSVIGLATNPTL
                     NPADPDYLWTDQGKVLSSQPGDDFNAIDPNILVDTDGSVWLTYGSYWGGIQQRRIDPE
                     TGKLSMTDTTRYSLAARPGVANDPVEGASLVQHNGYYYLFVSLDYCCNANYQTDNYKE
                     AVGRSTSPHGPFTDEQGVPMMQGGGTVLLKGDGVWMAPGGGTAYVDAG"
     gene            complement(1082..2662)
                     /gene="murJ"
                     /locus_tag="VFA02_04920"
     CDS             complement(1082..2662)
                     /gene="murJ"
                     /locus_tag="VFA02_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZU09223.1"
                     /translation="MTATTSSKTSSSKAAAVAVAAGIFSSRIVGLVRERVFAHYFGTS
                     AAADAFRAAFRIPNFLQNLFGEGVLSASFIPVYARLRAEGRDEEAAKLAEAIFALLFL
                     VTSVLVLGGIFAAPWLIDLIAPGFRGDTRLLTIHLVQVLFPGAALLVFSAWCLGILNS
                     HRRFFLSYAAPVVWNLTIVAVLLWKGGHQAEARLAVTTAIGSVIGSGLQFIVQLPTVL
                     KFLWPLRLHLQFASEQVRAVVHSFTPVFFSRGVVQISAYVDSMLASFLPQGAVAVLGY
                     AQTLTLLPVSLFGMAISAAELPAMSSELGTQEEIAAALRQRLARGLENIAFFVVPSAA
                     AFFILGDMVATTIYRSGHFQGHDVTLVWRVLAGSAVGLLASTSGRLYSSAFYALRNTR
                     TPLNFAIVRVTLTLALGYLFALPLPHLLGIDRLWGVAGLTLSAGIAGWVEFALLRRAL
                     HARIGAVPFSGARLAKLWLAAIVSAAVGYGVKLIFPIRQPLVIGPGVLAVFAGCYLVL
                     VSMAGVAMPGRLKNLLRR"
     gene            2771..>2987
                     /locus_tag="VFA02_04925"
     CDS             2771..>2987
                     /locus_tag="VFA02_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZU09224.1"
                     /translation="MEKLIAGYKKFRSEVFPEKKELFQQLAQTQSPQALFLTCSDSRI
                     VPDLVLQTGPGDLFICRDVGNIVPPYGE"
BASE COUNT          754 a          847 c          840 g          546 t
ORIGIN      
        1 ctgcctgcat ccacataagc tgtaccgccg cctggagcca tccacacacc atcgcctttc
       61 agcagaacag tccctccgcc ctgcatcatc ggcacaccct gctcatccgt aaatggacca
      121 tgcggactgg tagagcgccc aacggcttct ttgtagttgt ccgtctgata atttgcgtta
      181 cagcagtaat ccaacgaaac aaacagatag tagtagccat tgtgctggac gagcgaagcg
      241 ccctcgacag gatcattggc tacaccagga cgggcggcga gactgtatct cgttgtgtct
      301 gtcatcgaga gcttccctgt ctccggatca atgcgtcttt gttgaatgcc accccaatag
      361 ctgccatacg tgagccatac gctgccatcc gtgtccacca ggatgtttgg gtctatggcg
      421 ttaaagtcat caccgggctg cgatgaaagc accttgccct gatcggtcca cagataatcg
      481 gggtccgccg gattaagcgt cggatttgtt gccaggccaa tcacagagag ctgcgatcct
      541 gccgtggacg cggcatagta tacgtgataa agcccgtgga aataggaaac atccggcgcc
      601 cataggcaga gggtgcccgg gactgcgcct gtggtccatg ctggcatctg tttcggaaaa
      661 acgctgccgc atgaactcca gttcaccttg tcttgcgagc agcgaatcgg cagataattg
      721 ccgggaacaa gcgcaagcat gtcgctggta aaagcgtaat aggtatcgcc ctgacgaatg
      781 atgcttggat catgtaccgg ctgaacattc cccgttagcg catagttggc gagtgggtct
      841 gaggcagaaa gcggaatttg cggtggggat gcagaggccg cgggccttcc ggtaacgggg
      901 ccgcaggcac atacgagaga agcaaggaca gcgacagcaa aaagcgatgc agccttaaca
      961 acggctgaat tcatttttgt gggctttcac gaaggcaaca ctgagatgac gttaggctgg
     1021 aatcggttgc ttgtcaatca gatttttttt gatattgccg taagtctctc gatcgcaagg
     1081 cctaacgtcg caaaagattt tttagacgac caggcatcgc taccccagcc atactcacaa
     1141 gcaccagata gcatccggca aacacagcca gcacgcccgg gccaatcacc agcggctgcc
     1201 gtatgggaaa gataagtttc acgccatatc ccacggccgc ggaaacaatt gcagccagcc
     1261 agagtttggc cagacgggct ccggaaaaag gcacggcccc gatacgggca tgaagggcgc
     1321 gcctcagcaa cgcgaactcc acccatccgg caattccggc agaaagcgtc agtcctgcca
     1381 caccccacag gcggtctatt cccagcaggt gcggcaatgg caaagcaaac agatacccca
     1441 gcgcgagcgt caacgttacc cggacgatcg caaaattcag cggtgtgcgc gtgttgcgca
     1501 gcgcgtagaa agcagaagaa tagagccgcc ccgaggtgga ggcgagcagg ccaacggccg
     1561 accccgccag caccctccag acgagagtaa cgtcgtgtcc ctgaaagtgc ccggagcggt
     1621 aaatagttgt cgcgaccatg tcgccaagaa tgaaaaaagc agccgcggaa gggaccacga
     1681 agaaggcgat attttcaaga ccacgcgcaa ggcgctgacg cagggcggcc gcgatttctt
     1741 cctgcgtgcc cagttcgctc gacatcgcag gcagttcggc ggctgaaatc gccatgccga
     1801 agagactgac cggcagcagg gtcagggtct gcgcatagcc aagcaccgca acggcacctt
     1861 gtggcaggaa gctcgcaagc atagagtcta catacgcact gatctggacg acgcctcggc
     1921 tgaaaaatac gggcgtaaag ctgtggacaa ccgcacgcac ctgctccgag gcaaattgca
     1981 gatgcaggcg cagaggccac aaaaatttca ggactgtcgg cagttgcacg atgaactgga
     2041 gtccgctgcc aatgaccgag ccgatggctg ttgtaaccgc tagcctggcc tctgcctgat
     2101 gtccgccctt ccacaacagg actgcgacga tggtgaggtt ccacaccacc ggggctgcat
     2161 aagagagaaa aaacctgcga tggctgttga gaattcccag gcaccaggca gagaaaacca
     2221 gcagcgcagc gccgggaaac aggacctgga ccagatggat ggtgagcagg cgcgtgtcgc
     2281 cgcgaaagcc tggggcgatg aggtcaatca gccagggagc ggcaaaaatc ccgcccagca
     2341 ccagcaccga cgtaaccaga aacagcaacg cgaagatggc ttcagccagc ttcgctgctt
     2401 cctcgtcccg cccctcagcg cgtagacggg cataaacagg gataaatgaa gccgaaagca
     2461 cgccctcgcc aaagaggttc tgcaaaaaat tgggaatgcg aaaagcggca cggaaggcat
     2521 cggccgccgc cgaagtgcca aagtaatggg caaatacgcg ctcgcggacc agaccgacga
     2581 tacgacttga aaaaattccg gcagctacag caacggccgc cgctttcgag gaagaagttt
     2641 tgctggaagt ggttgcggtc aaaccatgat tatacgagtt ggattttgtg ccgggaatgc
     2701 tggctgtttc gcctttgtcc cgtcgaatgt ggtgccatcc atctccgtgc ttcagtaaga
     2761 taaaactgcc atggaaaaac tcatcgccgg atacaaaaaa ttccgttcag aagtatttcc
     2821 cgaaaagaag gaactgtttc agcaattggc ccagacgcaa agccctcagg ccctctttct
     2881 tacctgctcc gattcccgca tcgttcccga tctggttttg cagaccgggc cgggagatct
     2941 gttcatctgc cgcgatgtag gcaacatcgt gccgccttat ggagaaa
//
LOCUS       DASRBV010000128         6020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_614867, whole genome shotgun sequence.
ACCESSION   DASRBV010000128 DASRBV010000000
VERSION     DASRBV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614867"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFA02_04930"
     CDS             <1..555
                     /locus_tag="VFA02_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU09225.1"
                     /translation="GNEVPKEPLIFLKAPSSLLAHKQEIRLPAISQRVDFEGELAIVI
                     GKRCSKLGPDEDVRPYIRGYTIVNDVTARDLQKPDGQWWRAKGFDTFCPVGPLVTDEI
                     GPEAGVTVQTRLNGALKQSGNTRDFIFSIAHLLRHISAAMTLFPGDLIPTGTPSGVAP
                     MQAGDTVEVTIDGIATLSNPVVMG"
     gene            625..1629
                     /locus_tag="VFA02_04935"
     CDS             625..1629
                     /locus_tag="VFA02_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZU09226.1"
                     /translation="MPKTLLEQLRSMTTVVADTGDIEAIEKVKPQDATTNPSLITTAA
                     QMPQYQSIVDGVLLDAHKQLGDGASDKDVANLAFKHLAIEFGKRILQIIPGRVSTEVD
                     ARLSYDTEGTIKTAREIISLYEKAGISRERILIKIASTWEGIRAAEKLEKEGIHCNLT
                     LLFGLHQAIACAEAGVTLISPFVGRILDWYKKNTGKDYKGAEDPGVQSVTRIYNYFKK
                     FGYKTLVMGASFRNTGEIIELAGCDLLTISPQLLHELEATQGELPRKLDPEKAKTMDI
                     QKITVDKATFDKMHAEDKMASDKLSEGIEGFSKALENLEQLLAKRLCELESRQPVSAD
                     "
     gene            complement(1709..4762)
                     /locus_tag="VFA02_04940"
     CDS             complement(1709..4762)
                     /locus_tag="VFA02_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HZU09227.1"
                     /translation="MSIVSSRRDFLKGAATAGLLFSTRGLLVAEAQTPSATRELFLPD
                     EGWRMWPDTKAAWKEDTIYLPGEFSLSQLPVNPPTGGWAALSSSQGTSVTLPATVEQF
                     YWGAFGHRPYKNEYKFEETDHEVKNGSYCGVSWWWREIEIPEDFKGRRIFLHVRAARL
                     RAEVYLNQKLVGYSILEELPFECEITDAARPGERNQLAIRITNPGGEMDWVDGRELQW
                     GDVRLQSSHGFGGLDRALRLTAHGDVRVKDSWVLNTPDARAITVHAELQNLSTQPAAG
                     SIVFSVIDPASGHAVAKKDLPARVEANAAATFHADLRYDGAKLWDLDTPHLYHLRVEW
                     EGPGASSDFQQIWFGFRWFAPEGIGTNAMFRLNGRRTRIYTSISWGFWGVNGIFPTPE
                     LAKREVEAAKTFHLNCLNFHRNVGKPEVFDLQDRMGLLRCMEPGGGYQAVSASGFAQR
                     YMEAKIVHMIRAFRSHPSLVEYILQNEAKPDLKNPNLYRILRLMHQEDPSRSIVANDG
                     FASRSAQAWMGPYSDRVRTSAEGGAGGWWDEHQGPFSDVWNDRYYNGPDDFLYRSTDQ
                     GEIVEWGEMKGAASPDNHALLIQQIEQHGGKSYDLEDHKEILAAYEKFLDKWGFRTAF
                     PSASALFRSISRRAYESWGQFMENIRICDVNDYAAISGWESTAMENHSGLLDNMRDFK
                     TDPQLLAKSLLPVRPVAKQYHLVLRKGERAKFDIYLLNDTGRPVMGKLVFSITGPEGK
                     RTRLGTWACPEVEKDKLSYLVQKAVESDPLNGTGVWTTRLELNGSASSAHECRLWVVE
                     TAPYSLSGLKIGLIGAEQSLPFAGVTAEPYSASQKYDILLAMDAPQKSEPLVPTDDMG
                     AYRQSGQVREDVLPEGVIEQVRNGTPLVVVTGKDSIASGFAKQLAGAGAFTFYGMVGA
                     SRASWMGSWYFVRRHPLYAGMPVDEGMSIHYQVKGAGSNGWLVDGPQVEVVAGYSRDH
                     DRNIGAGTFTASLGQGRIVMQRIADMHPVFQERFLANALAYGMAR"
     gene            complement(4765..>6020)
                     /locus_tag="VFA02_04945"
     CDS             complement(4765..>6020)
                     /locus_tag="VFA02_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HZU09228.1"
                     /translation="VHVLTPGTYTFLGLDAQHKLGVTLDNVFADGLEQSKIIAKDAEI
                     TLRNRGNFAPGGEDVTVTESDTNKGRPLPCEGRFVPFPEIRTAPEAANTVLPEDKTFY
                     VAADGTGDYYSIQKALDEAPATGGATVLVAPGTYREVLHIDKPGIRLRSAKPDASRTV
                     VVNDLSAGTSGGTLHSATVNVTANDFFAENITFQNDWNRTHPQLPAGSQALALLVTGD
                     RAVFHNVRLLGNQDTLYTGSHCLAGETTCAPTRQYFSDCYIAGNVDFIFGDGKSFFDH
                     CEIHSTPHSEGFVTAQGKHEAKEDSAYVFHQCRLTADAGVTNVYLGRPWRPYATVIFL
                     NTWMGEHIQPAGWREWHPGETHSLDTAFYAEFQSTGPGAHTQERDPHTKFITQAEAAK
                     YTVQAFLSGNDHWDPIAVIAKNKLE"
BASE COUNT         1352 a         1826 c         1552 g         1290 t
ORIGIN      
        1 ggcaatgaag tcccgaaaga gccgctgatt tttctcaaag cgccgtcttc tctccttgcg
       61 cacaagcagg agatccgcct tccggcaatc tcgcagcgtg tggactttga aggtgaactc
      121 gccatcgtca tcggaaagcg ctgttcaaag ctcggcccgg acgaagacgt gcgcccgtac
      181 attcgcggat acaccatcgt gaacgacgtg actgcgcgcg acctgcaaaa gccggacggt
      241 cagtggtggc gcgccaaagg ttttgatact ttctgccctg taggtcctct cgtgactgat
      301 gaaattggtc ccgaagctgg agtgaccgtg cagacgcgtc tgaatggcgc gctgaaacag
      361 agcggcaaca cgcgcgattt catcttctct attgcgcacc tgctgcggca tatttccgct
      421 gcgatgacac tctttcccgg cgatttaatc cccaccggaa ctccgtcagg cgtggccccc
      481 atgcaggcag gagatacggt cgaagtcacg attgacggca ttgcaacact ctcgaaccct
      541 gtagtcatgg gataatgaaa agagaccgct atcagctctg cggcatccaa aagatagaca
      601 ccccgcgcca gatgaggagg aaccatgccg aagaccctgc ttgaacaact ccgctcgatg
      661 accaccgtcg tcgctgatac cggagacatt gaagccatcg aaaaggtcaa gccacaggat
      721 gcgaccacga acccgtcgtt gattaccact gctgcgcaaa tgccgcagta tcaatccatt
      781 gtggatggcg tgctgctcga tgcacacaag cagctcggcg acggggccag cgataaggac
      841 gttgccaatc tcgctttcaa gcacctcgcc atcgaatttg gcaagcgcat tctccagatc
      901 attcccggcc gcgtttcgac agaagtagat gcgcgcctct cctatgacac cgaaggcacc
      961 atcaagaccg cccgcgagat tatctccctc tacgaaaagg ccggaatttc gcgcgagcgt
     1021 atcctgatca agattgcttc cacgtgggaa ggcatccgcg cggctgagaa gcttgaaaaa
     1081 gaaggcatcc actgcaacct gacgctgctc ttcggattgc accaggccat tgcctgcgcg
     1141 gaagcgggcg tcacgctgat ttctccgttt gtgggacgca ttcttgactg gtacaaaaag
     1201 aacaccggca aggactacaa gggcgcagaa gacccgggcg tgcagtctgt gactcgcatc
     1261 tacaactact tcaagaaatt tggctataag acgctggtca tgggcgcaag cttccgcaac
     1321 accggcgaaa tcattgaact tgccgggtgc gatctgctga ccatctcgcc gcaactgctg
     1381 cacgagcttg aggccacgca gggcgagttg ccgcgcaagc tcgatccgga aaaagccaaa
     1441 acaatggaca tccagaagat taccgtagat aaggcaacct tcgacaagat gcacgctgaa
     1501 gacaagatgg cctcggacaa gctctccgaa ggcatcgagg gcttcagcaa agcgctcgaa
     1561 aaccttgagc agcttcttgc caagcgcctc tgcgaactcg aatcacgcca gcccgtcagc
     1621 gcggactgaa gcatatctgc aaccagatgc aaacaagggg tggcggttgc caccccttca
     1681 gtttcctgca caccagacag caaggcgatt accgcgccat tccatacgcc agagcattcg
     1741 ccaggaaccg ttcctgaaaa acaggatgca tatctgcaat cctctgcatc acaatgcgcc
     1801 cctgccccaa agaggcagtg aatgtgcccg cgccgatgtt gcggtcatgg tcgcggctgt
     1861 atccggccac aacttccacc tgcggtccat ccaccagcca tccgttcgaa ccagcacctt
     1921 tcacctgata atgaatgctc atgccttcgt ccaccggcat ccccgcatac agcggatgcc
     1981 ggcgcacaaa ataccatgac cccatccagc ttgcgcgtga agcaccgacc attccgtaaa
     2041 aggtaaatgc acccgcacca gcaagctgct ttgcaaaacc gctggcaatg ctgtcttttc
     2101 ccgtaaccac caccagtgga gtcccattgc ggacctgttc gatgactccc tccggcagca
     2161 cgtcttctct cacctggccc gattggcgat aggcccccat gtcgtccgtc ggcacaagag
     2221 gctcggactt ttgcggagca tccatcgcaa gaagaatgtc gtacttctga cttgccgaat
     2281 atggttcggc ggttacgcca gcgaagggca atgattgttc agccccaatc agaccaatct
     2341 tcagaccaga taaagaatat ggagccgtct ccaccaccca aagcctgcac tcatgcgcgg
     2401 atgacgcgga cccattcagt tcaagtcgcg tcgtccatac ccctgttccg ttcagaggat
     2461 cagactccac tgccttctgc acaagatatg acagcttgtc tttttctact tccggacacg
     2521 cccacgttcc aagcctcgtc ctcttgcctt caggacctgt aatagaaaac accagctttc
     2581 ccatcaccgg tctgcccgta tcgttaagca aatagatatc aaactttgcg cgctcgcctt
     2641 tcctgagcac caaatgatat tgcttcgcca cagggcgcac gggcaacagg cttttcgcca
     2701 gcaattgcgg gtccgtcttg aagtcgcgca tgttgtcaag caggccgctg tggttttcca
     2761 tcgctgtgga ttcccatccg ctgatggctg cgtaatcatt cacgtcgcag atgcggatgt
     2821 tctccatgaa ctgcccccag gattcatacg cgcggcggct gatggagcgg aagagcgcgc
     2881 tggccgaggg aaacgccgtg cggaatcccc acttgtcgag aaacttttca tatgcagcca
     2941 gaatctcttt gtggtcttca aggtcgtagc tcttgccacc atgctgctcg atctgttgaa
     3001 tcagcagcgc gtgattatcc ggcgaggccg cacccttcat ctcaccccat tcgacaatct
     3061 cgccctggtc ggtggagcgg tacaggaaat cgtccggccc gttgtaatag cggtcgttcc
     3121 agacatcaga gaacggccct tgatgctcgt cccaccatcc gccagcgccg ccttcagcag
     3181 aggtgcgcac gcggtctgaa tacggcccca tccatgcctg cgctgagcgc gaggcaaaac
     3241 catcattcgc cacaatggaa cggctcgggt cttcctgatg catcagcctg aggatgcgat
     3301 acagattggg gtttttcaga tcaggcttcg cttcattctg aagaatgtat tcgaccaggg
     3361 atggatggct gcgaaaggcc cttatcatgt gcacgatctt cgcttccatg tatcgctgcg
     3421 cgaagcctga ggcgctgacg gcctggtatc cgccgcccgg ttccatgcag cgcagcaggc
     3481 ccattctgtc ctgcaaatcg aagacctcgg gtttgcccac attgcggtgg aagttcagac
     3541 agttcagatg aaatgtcttt gctgcctcga cctcacgctt tgccagttcc ggcgtaggga
     3601 atattccgtt caccccccag aaaccccatg aaatggatgt gtagatgcgc gtgcgtcgtc
     3661 cattcaaccg aaacatcgcg ttcgtgccga tgccctcggg agcaaaccag cgaaagccaa
     3721 accagatctg ctgaaaatct gaagaggcac ctggtccctc ccattccacg cgcagatgat
     3781 acaaatgcgg agtgtccagg tcccagagtt ttgcgccgtc atagcgcagg tctgcatgga
     3841 aagtggccgc agcattcgcc tccacacgcg caggcagatc cttcttcgca acagcatggc
     3901 ccgatgcagg atctataaca gaaaacacaa tgctgcccgc ggcaggctga gtgctaaggt
     3961 tttgcagctc agcatgtaca gtgatggcgc gagcgtccgg cgtgttgagc acccaactgt
     4021 ctttcacccg gacatcgcca tgcgcagtca ggcgaagcgc gcggtccagc ccgccgaagc
     4081 catgactgct ctgcaaacgg acatcgcccc actgaagctc acggccatcc acccagtcca
     4141 tctcgccacc tggattcgtg atgcgaatgg caagctggtt cctttcgccg ggtcgggccg
     4201 catctgtgat ttcgcactcg aacggcaatt cttcgagaat gctgtagccc acgagtttct
     4261 ggttcagata aacctcggcg cgcaagcgcg ccgcgcgaac atggagaaaa atgcgcctac
     4321 ctttgaagtc ttccggaatc tcaatctcac gccaccacca tgagaccccg cagtacgagc
     4381 cgttctttac ttcgtggtcc gtctcttcaa atttatattc gtttttgtaa ggacgatggc
     4441 cgaacgctcc ccaatagaac tgctccactg tcgcaggtag cgtcacgctc gtgccttgcg
     4501 aagaagaaag ggccgcccat cctcctgttg gcggattcac tggcaattgc gacagagaaa
     4561 attcacccgg cagatagatc gtgtcttctt tccaagcagc tttcgtgtcc ggccacatac
     4621 gccagccttc atcgggaaga aagagttcgc gcgtggccga aggcgtttgt gcctctgcta
     4681 ccaacaggcc gcgtgtagaa aacagaagcc ctgcggtcgc cgcgcccttc agaaaatcac
     4741 ggcggctgga aacaatgctc atgtttactc cagtttgttt tttgcaatga cggcaatcgg
     4801 gtcccagtga tcattgccgc tcaggaatgc ctgcactgtg tactttgctg cctctgcctg
     4861 tgtaataaac ttcgtgtgcg gatcccgctc ttgcgtatgc gcccctgggc cagtggattg
     4921 gaattccgca tagaaggccg tgtccagcga gtgggtctct cccggatgcc actcgcgcca
     4981 tcctgcaggc tgaatgtgct cgcccatcca tgtattcaga aaaatcaccg tcgcataagg
     5041 gcgccacggg cgccccagat agacgtttgt cactccggca tctgccgtca gtctgcactg
     5101 gtggaagaca tacgccgagt cttccttcgc ctcatgcttg ccctgcgccg tcacaaatcc
     5161 ctcagagtga ggcgtgctgt ggatctcgca gtggtcgaaa aaagattttc cgtctccgaa
     5221 gatgaaatcc acattgcccg caatgtagca atcagaaaaa tactgccgcg ttggtgcaca
     5281 tgtagtttcc ccagcaagac agtgactgcc cgtatacagc gtgtcctgat tgcctagcag
     5341 acggacattg tgaaacactg cgcggtcacc tgtgacaagc aatgccagtg cctgcgaccc
     5401 agccgggagc tgcggatgcg tccggttcca gtcattttga aaagtaatgt tctcggcgaa
     5461 gaaatcattt gccgtcacat ttaccgtcgc ggaatgcaat gtcccgcccg atgtacccgc
     5521 gctcagatcg ttgacgacga ccgtcctgct tgcgtccggc ttagcactgc gcagacgaat
     5581 gccgggctta tcaatgtgca gcacctctcg gtatgttccc ggagcaacca ggacagtcgc
     5641 accgcccgtt gccggagctt catccagtgc tttctggatg gagtaatagt cacctgttcc
     5701 atcggctgcc acatagaagg ttttgtcttc cggcagaacg gtattggctg cctctggagc
     5761 agtccttatc tcgggaaaag gaacaaagcg cccttcgcac ggaagcggcc ttcccttatt
     5821 tgtatctgat tcagttacag ttacatcttc gccgcccgga gcgaaattgc cgcgattgcg
     5881 caacgtgatt tccgcgtctt ttgcgatgat cttcgactgc tccagcccat cggcaaaaac
     5941 attgtccagc gtaacgccga gcttgtgctg ggcatccagc ccaagaaacg tatatgtgcc
     6001 cggagtgagc acatgcacat
//
LOCUS       DASRBV010000129        24799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_107249, whole genome shotgun sequence.
ACCESSION   DASRBV010000129 DASRBV010000000
VERSION     DASRBV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 24799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24799
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107249"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2906)
                     /gene="bcsA"
                     /locus_tag="VFA02_04950"
     CDS             complement(<1..2906)
                     /gene="bcsA"
                     /locus_tag="VFA02_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HZU09229.1"
                     /translation="MKRALAFWNTLGTRSSLIAGVARFAVYAALLFLAIECITIRFNW
                     PEQLVLGTLTILLTYAIHRVSDAEVVTLALMFASMLATARYAYWRVATVAEAIAHQGS
                     ALGWANIFFMLVLLSAEFYAFVILFLGYIQTIRPLRRRPVPLPRNIEEWPHVDVLVPT
                     YNEPLSVVRATAFAALNMDYPQEKLHVYVLDDGRREEFRKFCEGAGVGYITRKDNKHA
                     KAGNINHALKLLGSPYVAIFDCDHVPTRSFLQVTLGWFLKDKKLGMLQTPHFFYSPDP
                     FERNLHQFMSIPNEGELFYGVVQDGNDLWNATFFCGSCAVLRRTALEEIGGIATETVT
                     EDAHTSLRMQMRGWNTAYINIPQAAGLATESLSSHVGQRIRWARGMVQVMRTDNPLTA
                     KGLKWPQRLCYFNAMVHFLYAGPRLVFLTAPLVYMLLGRINIPGYWIAIAAYALPHLF
                     LANVTNFRVQGRHRYSFWNEVYETVLAPYILGPTLLAFLNPKLGKFNVTAKGGIVKRS
                     YFDKRIALPYIILILLDVAALVVAPVRFFYWNAGHPGTVLMNVVWVLFNMVIVGTANA
                     VAFESKQLRKDVRISLKMPVQIKLPNGKSLFGETEDISRGGAWIKMEIPLDLPPATRV
                     QVVYPLRGQEAEFPAEVVRADGLNLRLHHAELSLEEEELLTLVLYSGADTWLSRSEFR
                     EPDKPMHSFLRLVRLSVKGVGLALSVLLPRRKAVSLKTARVEAVILLAAVLMGGCSFL
                     HAQSSVKGVSAPVQAEGTFHTSFSLKDLGVPEAVVFRGVASGRNISFALPPSNIAQQA
                     SLHLQYAFSPGLIPQMSHLSVLLNGTLVATVPVPQDRNQVQSNLSLTVPLPAELLVRN
                     NVLGFQFVGHYTQQCEDPANTTLWARVENTSQVEVSGSLLPLADDLKILPLPFYDGAV
                     SGTSAAVPFAFASSVADPAVQQAAGIVASWFGVTAKSRPLSFPVSTGDILPKGNV"
     gene            complement(2903..3961)
                     /locus_tag="VFA02_04955"
     CDS             complement(2903..3961)
                     /locus_tag="VFA02_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HZU09230.1"
                     /translation="MVNPQNQDGEVEVVDHELARDVARLYSWANVEEPVYRSFVRRRK
                     LHSSQTASDTEDRREEPKGHPSGISATAPLVEMEAPVSVPVPDVGAEKTRAASVQPAV
                     SQRQDPAHAAIAVYSLAGGVGKTTLCANLGRIFCSQGERVLLVDASGCGLLPFYYGAS
                     DFRPGLRTFVAPDGKYAPVQILGAEEMTSEWLETKVKPAMQKAQRVIFDLGPASAEML
                     PEILSMCAVVLVPVLSDLNSILTISRIESIWKHVRANGLRVPDPVYVFNEFDEQDPIE
                     QKARSLVARQVGERLLPQSIRYSAAVANAIADRMTVADHAPDSPVAQDYMELAYWLRE
                     AAPVAESPSVVRRRWSEQ"
     gene            complement(4366..5625)
                     /locus_tag="VFA02_04960"
     CDS             complement(4366..5625)
                     /locus_tag="VFA02_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZU09231.1"
                     /translation="MKAIIFAAGSDGEIHPHLGIGRALAARGHDVVFITTFNYVSVAR
                     ECGFDVLSFLGEEEKLEFLRGAEGLGPLAKIKQYCRFLADKATEICDLAASRIDRQTV
                     LIAPPFFYAIARLLHIRYATPYISTVLVPAHLYSLKSPPAFKSTQWFSGLPYFLRKPI
                     FFSGERLVLDPFFRMLLKGPCQHLDLPLPRRVISQWWYSPQKIVSLFYDWFCPAPDDW
                     PKQAMFAGFPMFYPNGTGTLSDGLLDFLAAGPAPVVFNPGTETQNPRLFFETALKTVQ
                     SLGVRAIFLTRLTEHLPPNLPETIWHETYTSLPLLLRRSSVLVHHGGIGTIALALEAG
                     IPQLVVPTWTDQLDNGRRVERLGCGVVQQKPLDSAALMEKLQYLLTSSQIRQRVDSMR
                     ARIEPGAKVCNRIAEMIEQSGPMMHDR"
     gene            complement(5711..7009)
                     /locus_tag="VFA02_04965"
     CDS             complement(5711..7009)
                     /locus_tag="VFA02_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZU09232.1"
                     /translation="MKRKLSAIEHMIDGNIAYIATVEGSVTPDRLRFALRQVQRKHPA
                     LRSVIREERDGLYYEEDCAPEITLRVLPRITEHDSQRECRAELAIPFAYGQPLLRIVL
                     LRAEQESDVLFISSHRICDGMSMLTIVREVLRLLYRDEDLVPYEAVTTRDMIGDYEPP
                     QPWKRKLTAHLLNGALRLVPVSHSKPENKEYYLEWSLDRFQTEALKQRCKAEGTSIHA
                     ALLVALGRSLFSVLGKKKLPAWIESPMDARRGRLARLKSDMLFFGGGSFKIQTEGCLE
                     KEFWTTAREINEEIRRKIEQELLDIPRRYHFNELLRPVPNGRIQTMVRAGDLLKVNGS
                     WNRFALSNLGNVVVSDDASPFRLKDLRIYVHSFNFRLLGLVIYTLHGEMRFYYAGDEN
                     CLGRDQADALRQVFTKLLKQQTGETAEERGFHYALSGTSG"
     gene            complement(7006..7986)
                     /locus_tag="VFA02_04970"
     CDS             complement(7006..7986)
                     /locus_tag="VFA02_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09233.1"
                     /translation="MKKFGVIFVLYQPTEEFLGNLERVSAICPTVVAVDNSPECDARL
                     HASLRERGIQVIFNRNQGGLAGAYNRGAETLLAQQCEVIFLLDQDSEIDGSFFEKMMQ
                     SCSELGRDEFLVGPKIYEVNLQRCMPVFEPGKRLPVRVRIDDCNEGMFPSLCVISSGS
                     AISAAAYRRLGPFREDYFIEYVDIEYSLRAISQNIPVYMNAAVTLRQITGKIERHGKH
                     YTTNHSAWRRYYGARNAVHCLRLHREHWGLHWVSTFLAYLQIVRVLQYEPQKLRKVTA
                     IVCGYLDGLFGRLGTFEDLHPHIAAFCKATERGQVSFDNNTSVTMAGDGA"
     gene            complement(7983..9401)
                     /locus_tag="VFA02_04975"
     CDS             complement(7983..9401)
                     /locus_tag="VFA02_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZU09234.1"
                     /translation="MKIAEYTSEDRQTTIAPRIPSPMTRRSRPLLLPERTMFREGRTP
                     FCSVFTVRLCGSLEEPQLWWALERVQNKHPLLRCVVDNDALNGPHFVLQEHPAPIPLR
                     IVERRNENDWQREARREWVTPFDTGRRPLLRLVWLRSITVHELLLIGHHCICDGQSGI
                     ILLRDLLAACDHPEQGIGAYDALGAMEDIVPADLLRSPRFQRRVRLKANMLRLAFHLK
                     RARRGEKPATRISHEQIYFHRWALDETWVQSLTERSRAENVTVLAPLALALMQAFRDV
                     RGAEGLRKTYAMVNARRFVPRLHPDALFGIVPGIELRMKDMPAPREISAGDFWLRARV
                     LKQEMTRRIARLGASVYNYLAALETLHDKYSRLVADTENAPAVRHLTISNMGRIDLPQ
                     QYRSFRLEAVYSPLVMVSPTPANTVVLSSFAGQMEFAIMSDEVSLPYADALKIQQRTM
                     EILDVCMEKPGLAYKSITEQRA"
     gene            complement(9398..10726)
                     /locus_tag="VFA02_04980"
     CDS             complement(9398..10726)
                     /locus_tag="VFA02_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZU09235.1"
                     /translation="MDRPLTMFERALYHGEGFHVNVMTTVRISGEITEQQIRQALARI
                     QEKHTVLRCLVVQKGGRPWFVQQEQPAPVPLRMIPRQTENDWLEAATLESLKRFDGSR
                     GPLARVVWLRGETVSELLLICSHALCDGRSLMTLLREILLLCDQPDADIGDPTTLNSL
                     DEVFPKEVLTDRHLQRRIRWKAAGAKLMLRLPRRATAWTYGKVYRCLWTLDEQASQLL
                     VARCKAENTNVFAALGLSCMLAFRDACGPKHIERFEAPVDFRRYLPNLRSDSLFAIAP
                     TITLSLQELRNTAPGPAGFWEMARALRGRMSRKVEKLKSTVFPLFLGIEHLHDVYDRM
                     AVYAQSKRAGRKVSLSYLGRVDGEQNYQKFRLEEICDISAMMTPTPANLIAIYSFDGR
                     FYFSLSSDESSLPYAKALEIKEQIISTLHACVADFAISGAMSVAVGAEAS"
     gene            complement(10753..12075)
                     /locus_tag="VFA02_04985"
     CDS             complement(10753..12075)
                     /locus_tag="VFA02_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09236.1"
                     /translation="MPEVKPVVIFTIGTQGDVRPCVALGQGLHRAGYPVRIATSSNFA
                     VMVRQAGLEFFPMTADFQAMLETDRSIADHGLNIRAMVRIFRERYACWAPHWVQEGLA
                     AAEGSGLLIGVSNSILLAKALSEALGIPFAIARLQPCTLSRILPPIMLIKSQRRLPGM
                     VSLAAHSVMFKLLWSVMRPAVNDIVRPRLGLPPYPWYGPYFRDLHQSKVINGFSQHVL
                     PRPADWTQNSQVTGYWFLDQNEWRPSDALLEFLAAGRKPVYIGFGSMVSNQVKAFTDT
                     VLEAVKKSGLRAVLATGWGGLEGKDGPQGDDIFFLRHAPHDQLFPLMAAAVHHGGAGT
                     TAAAVRAGIPSIIVPFFGDQPFWAARLNCLGVAPPALERKTLTPDALATALTAAQQPA
                     LVKKAELLGRVVRQEDGVSEAVRWLRVWNLLPATEPDITVEQSVEATA"
     gene            complement(12068..16099)
                     /locus_tag="VFA02_04990"
     CDS             complement(12068..16099)
                     /locus_tag="VFA02_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZU09237.1"
                     /translation="MIECDQSSFPLTVAQRGLWFSQKIHPDALMNIAEAVEICGPVKP
                     EVFQQALHRVISEAEQLRVSVIEEDGKPLQVLRSCYEGDLPYIDMSREPDPRAASEAW
                     MRDELRRPVDLAHDPLWTSALLKVDDNRYFWYQRAHHIVHDGYGGGLVARRVAEVYTA
                     YIQGEEPAPNRFCTVREMIEAETRYRSSDRFRRDREYWLEHLARMPEAVTLSRRNCRH
                     ELSSSLQRSTGHLPAAIVRQLAELGKQAGASLPQVLIGWIAAYYHRMTGAEDLVFRMP
                     VSGRLNPVLRSSVGVEANMVPVRLSFIPETTAADLFAQVSRVVLQALRHQQYRYEELR
                     RDLGLFGQEQNIAWLGVNIEPFDYRLNFDGAETITHNLSNSGAEDLTVFVYDRGTETD
                     VRFDLDANPDLYSMAELDEHRRRLMGLVEQLLAHPDKPLRQLDILGQEERRRLLVTWN
                     NTARALPYISLPTLVEQWAQKTPDAPAVVFGDAVVTYRQLNEYSTRYARHLLASGIKP
                     GDIVAVALPRNEQLLMVLLGIMRAGASYLPLDLDGPIERMLLVLADASPAALIAQAQV
                     HMRLASGDFLRLLPELPDTAFSDTPPAADLSSPDDVVYVLYTSGSTGKPKGVEVTHRN
                     LWNFLEGMQVELKPKASDRFLAITTLIFDIAGLELYLPLTVGACVVMGSSDALRNPPV
                     LARLVQRSGVTHMQATPSLWRILLANSKTKIEHVHALVGGEALSAELAARLKSMAARV
                     TQFYGPTETTIWSTAQELKEVGDTPPPIGRPILNTQLYVLDENQQLVATGVTGELYIG
                     GEGVAKGYHNKPELTAMRFLPDPFAGNGRRMYRTGDLVRWNEDGELEFIGRADNQVKI
                     NGHRVELGEIESVLLQHAIVAEAAVTAHRNDDGVISLHAYLVPLGGRPVDISSVRLFL
                     ASRLPNHMMPASFTVLDAMPLTPNGKLDRKALPVPERAITNIYVEPVTPTEKKLAQIW
                     RQILKVERVGLHDNFFDLGGDSLSAAEFIAHFHACFDMELPMGSLFEAPTVASLAAMV
                     ERLSSADTDPLGVVLPLRKIHQTTHRPLFCIHPMAGLSLGFSSLLRHLSSDLPVYGLQ
                     SRGLRGGHLPSSIEEIADDYLEQIRRIQPRGPYRLLGRSLGGLIAHCIAGQMELQELE
                     VELLAMIDSFLFLPDELSRPLTESDEVKAALSFMNIHFDEEKTQRTLRELGEFLLSPD
                     QGHPVPLAYQGMMRMRQMVMKNNPEFLENLTAVMLNNLKLARQYRPSRVGSGLLYFRA
                     TEMTGKVDDIIHRSPSAWQPFVGNMEVHDLACHHEAVLDPGPAAQMGKVLQQRFVHPD
                     VRQVLEIPRLVLEETGESSAAYA"
     gene            complement(16550..17917)
                     /gene="murF"
                     /locus_tag="VFA02_04995"
     CDS             complement(16550..17917)
                     /gene="murF"
                     /locus_tag="VFA02_04995"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZU09238.1"
                     /translation="MKLSLEQIARWTGGTLHGADGFSTGYSIDSRTLQSGDLFFAVRG
                     ERFDAHDFAGGALDRGACAAVVSKNKLRELPEAVRQHALIIVDEPLTALQTLAAAVRR
                     HWGKLVVGVTGSAGKTTTKEAIAAVLGAKFRVLKSQGNLNNGYGLPLQLLRLEQEHDI
                     AVIEMGMSNAGEIMALARIAAPNWGVVTNVGNAHAENFSDGIAGVARAKYELIASLPA
                     DGVAFLNCDDAYVSQFGRDFRGKTVYFGKGPCADPRAESIEELGTEGLRIRVRSGEQV
                     ADLRLQLLGQHNAANAMAAIAAGLEAGVPLRDCVSALAGLVPGDKRGQTFQVRGATII
                     NDCYNSNPEALKAMIQALMSIPGQRHILIAGEMLELGSEGASLHESCGEAAASAGVDL
                     VLGVRGSAERIADGAKKAGTEGIFLESPELAGAWLDRNLRAGDVVLLKASRGVRLERA
                     LEGLV"
     gene            complement(17914..19419)
                     /locus_tag="VFA02_05000"
     CDS             complement(17914..19419)
                     /locus_tag="VFA02_05000"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZU09239.1"
                     /translation="MKIHEALRGVEAVRCSGPEVEITGIEYDSRRIAPGALFVAMRGG
                     MTDGNRYIASAIERGASAVVTDTAAAFDETVQKYPQIAVTEVAHGRRTLAVVSANFFG
                     HPEKQLALSGVTGTNGKTTTAFLLDAMLNSAGRKTVLVGTIEYHVAGKVVESPHTTPE
                     SRDLLALLADGVKAGATEAVMEVSSHALEQGRVWSLPFDVAIFTNLTRDHLDYHGTME
                     NYFAAKAMLFDGRNGLPPRVAVVNVEDPYGAELAIKARETGAEVFSYGLGVGEFRAED
                     VSMSASGMGFTLMTPVGRGEIQTKLTGHVNVYNLLAASAAAYARGLSPEEIAMGAASL
                     KCVPGRFQAVDVGQPFTVVVDYAHTDDALRNLIRLAREFVKLQGGRVITLFGCGGDRD
                     RTKRPMMGRAAGEGSDFVVLTSDNPRSEDPEAIIRDALAGLKRTGVKFVTEADRAKAI
                     RIAVEEAKPGDIVLLAGKGHEKTQTLRDRVIRFDDAEVAAKAIRERMGAKA"
     gene            19534..19869
                     /locus_tag="VFA02_05005"
     CDS             19534..19869
                     /locus_tag="VFA02_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HZU09240.1"
                     /translation="MDPNTSILFVFSYLALFTGIALYLSPALVAILRTHPKRGSIFLL
                     NLLLGWTIIGWIAALVLARKSFVKPPPQKVNYKLLVEQVVLPCKPDYNFRAHGDHRAG
                     SRDLFPGNS"
     gene            complement(19786..22125)
                     /locus_tag="VFA02_05010"
     CDS             complement(19786..22125)
                     /locus_tag="VFA02_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZU09241.1"
                     /translation="MKQFSFQSGKKAKAQPPLSVEPGVVTPIRRLRLVYALAVFAVWA
                     GLIGLRLVWLQVIRHHEFVERAARQQQRTFEVAPRRGVLYDRNLHELAMTVLADSIYA
                     VPSELGDARAATAAALAKVVHTDASDSFTSEQQILARFNASKNFAWVARKQDPSIIAK
                     VKALNLKGVYFQKEFKRFYPDNDLAAQVLGYVGLDDTGLGGLEQNFDDELHGTPGRML
                     TAIDARRHVLGSEERDPLPGDNIVLTLDANIQFMAEQALDRNMERTHSESGTIVVQDP
                     HTGQILALAIRPTFNPNDFRHATTDLLKDHAVSDVYEPGSTFKPVTYSAALEEKAITP
                     DTMIDCGGGQLFIAGHTVHDAPGEHYGQVPAWKALAVSSNVGAIHVGQRIGPEKLYKY
                     IRAFGFGERSGIELPAETRGLLEPVKRWGPAEMGAIPMGQGIAVTPIQIATMVSTIAN
                     GGVYLPPHILLKSTELMKGDPRLQPVAFHPGNSLPDPLPDGAHRVISEMTAAEMRKMM
                     QDVVTIGTAHQAVHLNGYSAAGKTGTAQKIDPRTHTYSKTKFVASFVGFAPVNNPAIT
                     VVIVMDSPEHSMHFGAQASAPVFQDLAQQILEYLGVPHDQEMTPQTMSARNQPPVAED
                     DNPEESNGDLNSLFATVNDLPADDPLRAPQQAPGEAAPVAKDVAQAQAPTPQQDASGG
                     RMGGLPAKSAFETPAPPMSAAVVTSPAPQTKGPVVVNAGKQVAVPLFTGQTVRNVIEN
                     AEAAGLGVQVLGSGIAREQVPAAGTMVPVGTEVVVRFTR"
     gene            complement(22438..22998)
                     /locus_tag="VFA02_05015"
     CDS             complement(22438..22998)
                     /locus_tag="VFA02_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZU09242.1"
                     /translation="MKKEPELVARGWQETQMATQVIATHAVITGGRKGIEAVAAERNL
                     SLMAQQQRARRGPTPEVFFAKYFDNSRLVKAADPQRVKEMRSFACAMATLLLLVMVYG
                     WQHFSAIEYGYRVEAEKQQVQQLEEQNRQLRLTEAQLASPERIDKIARQYGLQAPAPG
                     QVVRPDGMADPNAPVMAKMTPVAPVR"
     gene            complement(23193..24122)
                     /gene="rsmH"
                     /locus_tag="VFA02_05020"
     CDS             complement(23193..24122)
                     /gene="rsmH"
                     /locus_tag="VFA02_05020"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZU09243.1"
                     /translation="MTERERHVPVLFKEAIRYLNVRPGGTYCDATLGLAGHSTAIARQ
                     IGPLGTLIAFDRDPEAMALARGRLDALRETLGPAMPKVVLHDVAFSEAAHLIGPESLD
                     GLLADFGVSSMQFDEAHRGFSFQAEGPLDMRMDPRQGLTAEQVVNQIGEKELADLIYE
                     FGEERRSRRIARAIVRARPVTTTAQLARVVAAAAPAMKSERIHPATRTFQALRIYVNA
                     ELDEIRALLEAAPRLLKRGGRLVAISFHSLEDRLVKDALREGARQGVYEVLTRKPVTA
                     EAEETDRNPRARSAKLRAAERKTVVSYKSPVSE"
     gene            complement(24150..24590)
                     /locus_tag="VFA02_05025"
     CDS             complement(24150..24590)
                     /locus_tag="VFA02_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZU09244.1"
                     /translation="MFRGNHPARVDEKGRLKLPAEFKRRIEESYGTQFYITSKDGRVA
                     EIYPLQEWEKIEQKIAAIPSFNPAKKKLLDRVNYYGQMAEMDQQGRVLIPQILRQTAK
                     VEGDVVVFGMQTYLEVTNRESFEHKLATEPLTEDDAQTLASFGL"
BASE COUNT         5615 a         7609 c         6163 g         5412 t
ORIGIN      
        1 acattgccct tcggcagaat gtctcccgtg cttaccggga aactcagcgg cctcgatttt
       61 gccgtcactc cgaaccatga ggccacgatc ccggcggcct gctgcacagc cgggtctgcc
      121 acagaagaag caaaggcaaa ggggaccgcg gccgaggtcc cgctcaccgc gccatcgtaa
      181 aacggcagcg ggagaatctt cagatcatcg gccagaggca gcaatgagcc tgacacctcg
      241 acctgtgaag tattttccac ccgcgcccaa agggtagtgt tggccggatc ttcgcactgc
      301 tgcgtgtaat gccccacaaa ctggaacccc agcacgttgt tgcgcaccag cagctctgcc
      361 ggaagcggca ctgtcagcga tagattgctc tgtacctggt ttcggtcctg cggtaccggc
      421 accgttgcca ccagagtgcc gttgagcagc acgctcagat ggctcatctg cggaatcagc
      481 ccaggcgaaa acgcatattg cagatgcagt gacgcctgct gcgcaatgtt cgacggcggc
      541 aacgcaaagc tgatgttgcg ccccgaggcc accccgcgaa agaccactgc ctccgggacg
      601 ccaaggtctt tcagcgaaaa gcttgtgtga aaagtccctt ctgcctggac cggcgcagaa
      661 acacctttta cagaagattg tgcatgaaga aaactacatc ctcccatcaa cacagccgcc
      721 agcaaaatca cggcttccac ccgcgccgtc ttcaggctga cagcctttct tctgggaaga
      781 agaacagaca aagcaagccc aacgcctttc acggaaagcc gcacgaggcg tagaaagctg
      841 tgcatcggct tgtctggctc gcggaattcg ctgcggctca accatgtgtc tgcgccggaa
      901 taaagcacca gagttaaaag ctcctcttct tcaagagaca gctcggcatg atgcagacgg
      961 agattcaacc cgtctgccct gaccacttcc gcaggaaatt ccgcctcttg tccacgtaac
     1021 ggatagacca cctgcacacg cgttgcaggt ggcaggtcaa ggggaatttc cattttgatc
     1081 cacgcgcctc cgcgagaaat gtcctcagtt tcaccaaaga ggcttttgcc attcggcagt
     1141 tttatctgca cgggcatttt tagagaaata cgcacgtcct tgcgcaactg cttcgattca
     1201 aaggccacag cattcgccgt tcctacaatg accatgttga acagcaccca gacgacattc
     1261 ataaggactg tcccgggatg gcccgcattc cagtagaaaa agcgcacagg agctaccacc
     1321 agtgcggcga catccagcaa aatcagaatg atgtatggaa gagcaatacg tttgtcgaaa
     1381 tagcttcttt tgacaatgcc gccctttgct gtcacgttga acttgcccag ctttggattg
     1441 agaaatgcca gcagagttgg accaagaata tagggtgcga gcactgtttc atacacttca
     1501 ttccagaacg aatatctgtg ccttccctgc acccggaagt tcgtgacatt ggcaagaaac
     1561 aggtgcggaa gtgcatatgc ggcaatcgca atccagtaac ctgggatatt gatcctgccc
     1621 agaagcatgt agaccagcgg tgccgtaaga aaaacaagcc ggggtccggc atacagaaaa
     1681 tgcaccatcg cgttgaaata gcacagccgc tgtggccact tcagcccttt cgctgtcagc
     1741 ggattgtccg tccgcatcac ctgtaccatg ccgcgcgccc agcggatacg ctgccccaca
     1801 tggctggaaa ggctttccgt cgccagccct gccgcctgcg gaatattgat gtatgccgtg
     1861 ttccatcccc gcatctgcat ccgcaatgag gtgtgcgcgt cttctgtgac cgtttccgtc
     1921 gcaatcccgc caatctcctc cagcgccgtc cggcgcagca ccgcgcacga tccacagaaa
     1981 aacgtcgcgt tccacaaatc attgccgtct tgcaccactc cgtaaaacag ctccccttca
     2041 ttgggaatag acatgaactg gtgcaggttc cgctcaaacg gatcaggcga ataaaagaag
     2101 tgtggtgttt gcagcattcc cagcttcttg tctttcagaa accagcccag cgttacttgc
     2161 agaaaactgc gcgtcggcac gtggtcgcag tcgaaaatag cgacatacgg agaaccaagc
     2221 agcttcagtg catgattgat gtttcccgct ttggcgtgtt tattgtcctt gcgggtgata
     2281 tagcccaccc ccgctccttc gcagaacttg cggaactcct cgcggcggcc atcatccaga
     2341 acatagacat ggagcttttc ctgcggataa tccatgttca aagcagcaaa tgccgtggca
     2401 cgcacaaccg aaagcggctc gttatacgta gggaccagaa catccacatg cggccattct
     2461 tcaatgttgc gcggcagtgg aaccggtctt cggcgcaacg ggcgtatggt ctggatatac
     2521 ccaagaaata gaatgacgaa tgcgtagaac tcggctgaaa gcagcacgag cataaaaaag
     2581 atgttggccc agcccagcgc gctgccctgg tgcgcaatcg cctccgccac cgtcgcaaca
     2641 cgccaatatg cataccgcgc cgtcgccagc atggaagcaa acatgagggc aagagtcaca
     2701 acttctgcat cagatactcg atggatggca taggtgagca gaatggtcag cgtgcccagc
     2761 accaactgct ccggccagtt gaaacggatg gtaatgcact caatggccag gaaaagcagc
     2821 gcggcataaa cggcaaagcg cgccacaccg gcaatcaggc tggagcgggt gccaagggtg
     2881 ttccagaagg ccagcgcccg tttcattgct cgctccaccg cctgcgaact accgacggtg
     2941 attcagcaac cggggcggcc tcgcgcagcc aataggccag ctccatataa tcctgggcca
     3001 caggcgaatc cggtgcatgg tcggcaaccg tcattcggtc tgcaatggcg ttggccacag
     3061 ccgcactgta ccggatggac tgaggcaata accgctcacc tacctggcgc gctaccagcg
     3121 atcgcgcctt ctgctcaatc gggtcctgtt catcgaattc attgaagaca taaaccggat
     3181 caggaacgcg aagaccattc gcgcgcacgt gtttccatat ggattcaatg cgcgaaatcg
     3241 tgagaatgga gttcaggtct gagagcacag gcaccagtac gacagcgcac atactcagaa
     3301 tctccggcag catctccgcc gatgccggcc cgaggtcgaa aatcactctc tgcgcctttt
     3361 gcatggctgg tttcaccttt gtctccagcc attcactggt catttcctct gcccctaata
     3421 tctgtaccgg agcatatttg ccgtccggcg ccacaaacgt cctcagtccc gggcggaagt
     3481 cgctggcgcc ataataaaag ggcaacagcc cacagcctga tgcatccacc agaaggaccc
     3541 gttccccctg agaacagaag atgcgcccaa gattcgcgca caacgttgtc ttccctactc
     3601 caccggccag cgagtaaaca gcaatggccg catgggcagg gtcctgccgc tgcgacacgg
     3661 ctggctgcac tgaggccgcg cgggtcttct cagccccaac atcaggcact ggtacagaaa
     3721 caggagcttc catctcaacc agaggcgcag tcgccgaaat ccctgaaggg tggcctttgg
     3781 gctcttctct acggtcctca gtatcgctgg ccgtttgact ggagtgcagt tttcgcctcc
     3841 gcacaaaact gcgatacacg ggttcttcca cattcgccca tgaatacaat cgcgccacgt
     3901 cgcgcgctaa ttcgtggtcc acaacttcta cttctccgtc ctgattctgc ggatttacca
     3961 tccgtcacac acctggcctg ttgcgattta gagttccttg cggatggaat tggacttcgt
     4021 atgcgttttc ttcactcaaa caccaagtct gagacgacag catccgcaca cacatgtacc
     4081 tttcccgcac actggggtaa agttgcaagt acagcagtgt atgaaactta gatttttatt
     4141 taacttttga ttcctactgt acacagttcg gcctctccat gcacgaacaa tttaagaagt
     4201 tattctcagg tagtagtcga aacgggaaaa catcccaaaa cagaacgcaa gctgctgaaa
     4261 ttcaacagtt taatatggaa acctttccac ttcctggcct gcgaaactat ctcgatttcg
     4321 ccataaaatc accgtccctt caccattctc atatccacct tctcattacc tgtcatgcat
     4381 cataggccca ctttgctcaa tcatctctgc aatgcgatta catacttttg cgcctggctc
     4441 gattctcgcc cgcatcgagt caactctttg ccggatttgt gatgaagtta acaggtactg
     4501 taatttctcc atcaaagcgg cgctgtccag cggcttctgc tgtactactc cacaaccgag
     4561 ccgctccacc cgccggccat tgtccagttg gtccgtccat gtggggacaa ccaattgcgg
     4621 aatacctgct tcgagcgcca gagcaatcgt gccaataccg ccatgatgta ccagtacgct
     4681 gctcctccgc agcaggagcg gcaaagaagt gtatgtctca tgccatatag tttcaggtaa
     4741 attcggcggc aaatgctcgg taagccgcgt aagaaaaatg gcccgcaccc caagtgactg
     4801 gacagttttg agagcagttt caaaaaacag gcgaggattt tgcgtctccg ttccaggatt
     4861 gaagacaaca ggagccggcc cggcagcaag gaaatcaagc agcccgtcgc tcagtgtccc
     4921 ggtgccgttc ggataaaaca taggaaaacc ggcaaacatc gcctgcttcg gccaatcatc
     4981 cggggctgga cagaaccagt catagaacaa gctgacaatt ttctggggag aataccacca
     5041 ttgactgatg acccgtctgg gcaggggcag atccagatgt tgacaaggcc ccttcagcaa
     5101 catgcggaag aaagggtcca ggaccaggcg ttctccgctg aagaagattg gcttacgtaa
     5161 gaaataaggt aggcctgaaa accactgcgt ggatttgaac gctggcgggc tttttaatga
     5221 ataaaggtgt gcagggacca gcacggttga gatgtatggt gttgcgtatc gaatgtgcag
     5281 caacctggca atcgcgtaaa agaaaggcgg ggcgatgaga acggtttgac ggtcgatccg
     5341 gctggcagcc aggtcgcaaa tctcagtagc tttatcggcc aggaacctgc agtactgctt
     5401 gatctttgcc aatgggccca gaccctccgc gccgcggaga aactccagct tttcttcttc
     5461 gccaagaaaa ctcagaacat caaacccgca ctcgcgcgct acagagacat agttaaacgt
     5521 agtaatgaaa actacatcgt gaccacgtgc tgctaatgcg cggccaatcc ccaggtgagg
     5581 atgaatttcg ccgtccgacc cggctgcaaa aataatcgcc ttcatagaaa ttgagcattt
     5641 accgaaacat cgccatatat cggacctcaa gccggaccag cgaatcaatc cccttacagg
     5701 gcatgtagca ctagccagag gtcccactca acgcataatg aaatccacgc tcctctgctg
     5761 tttcccctgt ctgctgcttc aataatttcg tgaacacctg acgaagcgca tccgcctgat
     5821 cgcggcccag gcaattctca tcgccggcat agtaaaagcg catctcgcca tgcagtgtat
     5881 aaataacaag cccaagcagc ctgaaattga aggaatgcac ataaatgcgc aggtccttta
     5941 agcgaaatgg agatgcatcg tcactcacca caacattgcc cagattggac aaagcaaacc
     6001 ggttccagct tccattcact ttcagcaagt ctcctgcccg caccatggtc tggatccgcc
     6061 cattcggcac aggccggagc agttcgttga agtggtaccg gcggggaata tcgagcagtt
     6121 cctgctcgat ttttctgcgt atctcttcgt ttatttcgcg cgccgtcgtc cagaattctt
     6181 tctcaagaca tccttctgtc tgtatcttga aactccctcc accaaagaac agcatgtcgc
     6241 ttttcaatct cgccagacgg ccgcgacggg catccattgg gctttcaatc cacgccggca
     6301 acttcttttt gcccagcaca gaaaagagag agcggcccaa tgcgaccagt aaagctgcat
     6361 gaatggaagt accttcggcc ttacagcgtt gcttcagtgc ctctgtctga aagcggtcga
     6421 gactccactc cagatagtat tccttgttct ccggtttgct gtgcgagacc ggaaccagcc
     6481 gcagcgctcc attcagcaga tgcgctgtca gctttcgttt ccacggctgc ggaggctcat
     6541 agtctccaat catgtcgcgt gtcgtgaccg cctcatatgg caccagatcc tcatccctgt
     6601 agagcagccg taatacttct ctcacgatcg tgagcatgct cataccatcg catatgcggt
     6661 gtgaagatat aaacaataca tcgctttcct gttcggccct taacaacaca atccgtaaca
     6721 gaggttggcc ataagcaaag ggaattgcga gttcagcccg gcactcgcgc tggctatcgt
     6781 gctccgttat acgcggcagg acccgtagag tgatttccgg agcacagtcc tcttcgtaat
     6841 acagcccatc cctttcttcc cgaatgaccg aacgcagagc aggatgcttg cgctgcacct
     6901 gccgcagcgc aaagcgaagt ctgtcgggtg taacacttcc ttccacagtg gcaatatagg
     6961 caatattgcc gtcaatcatg tgctcaatgg cagagagctt ccgcttcacg cgccatctcc
     7021 tgccatcgtt acagatgtgt tgttgtcgaa cgatacctgt ccgcgctccg ttgctttgca
     7081 gaaggcagct atgtgcggat gcaggtcttc aaatgtaccg agccggccaa acaatccgtc
     7141 gagataacca caaacaatcg ctgtgacctt cctcagcttc tgcggctcgt attgcagcac
     7201 gcgcacaatc tgcagatatg ccagaaatgt gcttacccaa tgcagtcccc agtgttcgcg
     7261 atgcagacgc agacaatgca cggcattgcg cgccccgtaa tatcgccgcc atgccgaatg
     7321 gtttgtcgtg tagtgtttgc catggcgctc gattttgcct gtaatctgcc gcagtgttac
     7381 agcagcattc atatatacgg gaatgttctg gctgatggcg cgcagactgt attcgatatc
     7441 aacatactcg ataaaatagt cttccctgaa aggcccgagc ctgcggtaag cggcagccga
     7501 aatggcagaa ccggaagaaa tgacacacag ggacgggaac attccttcat tgcaatcatc
     7561 aattcgcacc cgcacaggga gacgcttgcc aggctcaaat acaggcatgc acctttgcag
     7621 gttgacctca tatattttgg ggcccaccag aaactcatct ctgcccagct cgctgcatga
     7681 ctgcatcatc ttctcaaaaa acgacccgtc aatctcagag tcctggtcaa gcagaaaaat
     7741 gacctcgcac tgttgcgcca gcagtgtttc ggcccctctg ttataagctc cggcgagtcc
     7801 tccctgattg cgattaaaga tgacctgaat tccccgctcc cgcaaagatg catgcaggcg
     7861 ggcatcgcat tccggagagt tgtccacggc caccaccgtc gggcagatag cgctcacacg
     7921 ctcaagattg cccagaaatt cttccgttgg ctgataaagc acaaagatga caccgaattt
     7981 tttcatgccc tctgctctgt gatggatttg taggcgaggc ccggtttctc catacataca
     8041 tccagtattt ccatcgtccg ctgctgaatt ttgagagcat ccgcataagg cagtgagact
     8101 tcgtctgaca taatcgcgaa ctccatttgt ccggcaaaac tggacaggac aacggtattt
     8161 gcaggagtcg gcgacaccat gaccagtggg ctgtacaccg cctcaagtcg aaagctccgg
     8221 tattgctgcg gcaggtcaat cctccccata ttcgatatcg tgagatggcg cacggcgggg
     8281 gcattttctg tatctgcaac aagccgcgaa tacttgtcat gcagagtttc cagcgcagcc
     8341 agatagttgt acacgctggc gccaagacgt gcgatgcggc gcgtcatctc ttgtttcaac
     8401 acgcgcgcac gcagccagaa gtcgcccgcg gagatttctc gtggcgcagg catatctttc
     8461 atgcgtaatt caatcccagg cacgattcca aacagggcgt ccggatgcaa ccgcgggaca
     8521 aacctgcggg cattcaccat ggcgtaggtc ttgcgcaatc cctcggcgcc gcgcacatcg
     8581 cgaaatgcct gcatcaaagc gagtgccagc ggagcaagca cggttacatt ctccgctctg
     8641 gagcgctctg tcagggattg cacccatgtc tcatcaagcg cccaccggtg aaaatatatc
     8701 tgctcgtggg aaatgcgcgt cgcgggcttc tctccgcgcc gggccctctt caggtgaaag
     8761 gccagtcgca gcatgtttgc tttcagccgg acacgacgct gaaagcgcgg gcttcgcagc
     8821 aggtccgcag gcacaatgtc ctccatcgcg ccaagcgcat cgtaagcgcc aatgccttgt
     8881 tccggatgat cgcaggcggc aagtaaatcg cgcaggagga tgatgcctga ttggccatcg
     8941 cagatacagt ggtgaccgat gagcaataat tcgtggaccg ttatggatcg cagccacacc
     9001 agacgcagta agggcctgcg ccctgtatcg aagggggtca cccattcgcg gcgggcctct
     9061 ctctgccagt cattttcatt cctgcgctca acaatacgca gcggaatcgg cgcagggtgc
     9121 tcctgtagca cgaaatgcgg accgttgaga gcatcattgt ccacaacaca gcgcagcagc
     9181 gggtgtttgt tctgtacacg ctccaacgcc caccacaatt gaggttcttc taaactgccg
     9241 caaagcctga ccgtgaaaac actacagaac ggcgtacgcc cctcacggaa cattgtgcgt
     9301 tccggcagga ggagcggacg tgagcgtcgg gtcatcggag aaggaatgcg tggcgcgatg
     9361 gttgtctgtc tatcctccga tgtgtactcg gcaatcttca tgatgcctcc gcgcccactg
     9421 caactgacat ggctccgctg atggcaaaat cagcaacgca ggcatggagt gtgctgataa
     9481 tttgttcttt gatctcaagg gccttcgcat agggcaatga cgattcgtcg gaagaaagcg
     9541 agaaataaaa cctgccgtca aaactatata tggcaatcag attggctggg gtcggcgtca
     9601 tcatggccga aatgtcgcat atctcctcaa gacggaattt ctgataattc tgctctccgt
     9661 ctactctccc caggtagctg agcgagacct tccgcccagc gcgtttggat tgggcgtaaa
     9721 cggccatgcg gtcatataca tcatgcaaat gctcgattcc cagaaaaaga ggaaaaacag
     9781 tggatttcag cttctctacc ttacgggaca tcctgcccct gagcgctcgc gccatctccc
     9841 agaaaccagc cggccccggt gctgtgttac gtaattcctg cagcgacaaa gtgatggttg
     9901 gcgcaatggc aaacaggcta tcgcttctca ggtttggaag ataccgccgg aaatccaccg
     9961 gggcctcgaa cctctcaata tgcttgggcc cgcaggcatc gcgaaaggcc agcatacatg
    10021 aaaggccaag tgcagcgaac acgtttgtgt tctcagcctt gcaccgcgcc accagcaact
    10081 gtgatgcctg ctcatcgagc gtccacaggc aacgatagac cttgccatag gtccatgcgg
    10141 tagcgcgccg cggaagccgc aacatcagct ttgcgccagc tgctttccaa cgaatccgcc
    10201 gttgcaggtg gcggtccgtc agcacctctt ttggaaatac ctcatcgaga gaattgagtg
    10261 tggttgggtc accgatatct gcatcgggct gatcacataa caaaagaatc tcgcgcagaa
    10321 gcgtcatcag cgaccgtcca tcgcagagag catgactgca aatcagaagc agttcgctca
    10381 ctgtttcacc ccgcagccag accacccggg ccaatggtcc tctactgccg tcaaaacgct
    10441 tgagtgattc cagagtagct gcttccagcc agtcattctc tgtttggcgt ggaatcattc
    10501 gcagtggtac cggtgcaggt tgttcctgct gcacaaacca tgggcggcca cccttctgaa
    10561 caaccagaca gcgcaaaacg gtgtgctttt cctggattcg cgccagtgct tgcctgattt
    10621 gttgttctgt tatctctccg ctgatgcgca ccgtcgtcat cacattcacg tggaaaccct
    10681 cgccatggta gagcgcgcgc tcaaacatcg tcagcggacg gtccaagctt ctgtttcctt
    10741 cgcgcatttc cgtcatgccg tggcctcgac actctgttcc accgtaatgt ccggctctgt
    10801 cgccggaagc agattccata cccgcaacca acgcaccgct tcactgacgc catcttcttg
    10861 gcgtacaacc cgccccagca attctgcttt ctttaccaac gctggttgct gtgcggcagt
    10921 cagcgcggtg gccaacgcgt caggagtcag cgttttgcgt tccaacgcag gcggggccac
    10981 gccaagacaa ttcagacgcg cggcccagaa tggctgatcg ccaaagaacg gcacaatgat
    11041 ggatggaatc cctgcgcgca ccgcagccgc agtcgttccg gccccgccat gatgcaccgc
    11101 cgcagccata agcggaaaca actggtcatg cggagcatgg cgcagaaaaa aaatatcgtc
    11161 gccctgcgga ccatctttgc cttcaagacc gccccaccct gtggccagca cagctcgcag
    11221 accgctcttt tttactgctt caaggactgt gtccgtaaat gccttcacct gattgctcac
    11281 catgcttcca aagccgatat agacaggctt ccggcccgca gccagaaatt cgagcaatgc
    11341 atcggaagga cgccattcat tctggtccag gaaccagtaa cccgtcacct gagaattctg
    11401 cgtccagtcc gcgggacggg gcagcacatg ctgactgaaa ccgttgatca cctttgactg
    11461 gtgcagatca cggaaatacg ggccatacca cggataagga ggcagtccaa gccgaggccg
    11521 gacaatatcg ttcaccgccg gacgcatcac actccataac aacttgaaca tcacagaatg
    11581 cgccgcgaga ctcaccatgc ccggcaatct gcgctgagac ttgatcagca taatcggcgg
    11641 caaaatccgc gagagtgtgc acggttgcaa ccgcgcgatc gcgaaaggaa tgccaagcgc
    11701 ctccgagagt gctttggcca gtagaatgct gttgctgacg ccgatcaaca gccctgagcc
    11761 ttccgccgcc gccagtccct cctgcaccca atggggggcc cagcacgcat agcgctcgcg
    11821 gaagatgcgg accatcgcgc gtatgttcag gccatggtcg gcaatggaac ggtccgtttc
    11881 cagcatggcc tgaaaatcag cggtcatagg aaagaattcc agtcctgcct gacggaccat
    11941 cacagcgaaa ttactgctgg ttgcgatgcg gaccggatac cctgcgcgat gcagcccctg
    12001 ccccagagct acacacggac gaacatcgcc ctgcgttccg atggtaaaaa tcactacagg
    12061 ctttacttca ggcatatgct gctgagcttt ctcctgtttc ttcaaggaca agccggggaa
    12121 tctccagcac ctggcgaaca tccggatgga cgaagcgctg ctgcagtacc ttgcccattt
    12181 gtgcggcagg ccctggatca agcacggcct cgtgatggca ggccagatcg tgtacctcca
    12241 tatttcccac gaatggctgc cacgccgatg gactgcgatg gatgatgtcg tccaccttgc
    12301 ccgtcatctc tgtcgcgcga aaatacagca agccggatcc aactcgagat ggccggtatt
    12361 gccgcgccag tttcaggttg ttgagcatca ccgcagtcag gttctcaagg aactccggat
    12421 tgtttttcat caccatctgc ctcatcctca tcattccctg ataggccaga ggcactggat
    12481 ggccctgatc gggactcagc aaaaactctc caagctccct cagagtgcgc tgcgtctttt
    12541 cttcatcaaa atgaatattc atgaagctca gcgctgcctt tacctcatct gattcggtaa
    12601 gcggccggct taattcatca ggaagaaaca aaaagctgtc tatcatcgcc agcaattcca
    12661 cttccagttc ctgtagctcc atctggccgg caatgcagtg agcaatcaag ccacccagtg
    12721 aacggccaag aagccggtaa ggccctctgg gctggatacg tcgaatctgc tcgaggtaat
    12781 cgtctgcaat ctcttcaatg ctcgaaggaa gatggccgcc acgcagcccg cgcgactgca
    12841 ggccatatac aggcagatcg gaactcaaat gacgcaacag gcttgagaag cccaggctga
    12901 gccccgccat aggatgaatg cagaacaaag gacggtgcgt agtctgatgg attttacgca
    12961 atggcagtac gacgcccagc gggtcggtgt cagcactgct gaggcgctcg accatcgctg
    13021 ccaggctggc aacagtaggt gcctcaaaca aactgcccat tggcagttcc atatcgaagc
    13081 aggcatgaaa atgtgcgatg aattcagccg ctgaaagcga gtcgccacca agatcaaaaa
    13141 aattatcgtg cagacccact cgctccactt taagaatctg acgccatatc tgtgccagtt
    13201 tcttttccgt aggcgtcacc ggttcaacat aaatgttcgt gatcgctcgc tccggcacag
    13261 gcaatgcctt gcggtccagc tttccgttcg gtgtcagggg catggcatcg aggacggtaa
    13321 aactggctgg catcatgtgg tttggcaggc ggctggcaag gaacaggcgc acggagctga
    13381 tgtccaccgg cctgccgccc aatggaacca gataagcatg aagcgatatc acaccatcat
    13441 catttcgatg tgcagtaact gctgcctcgg caacgattgc gtgttgcagc aaaacacttt
    13501 caatctcgcc cagctcaacg cggtgaccgt tgatcttcac ctgattgtct gcgcggccaa
    13561 taaattcaag ctctccatcc tcgttccagc gcaccaggtc ccctgtgcga tacatgcggc
    13621 ggccattccc tgcaaacgga tcgggaagaa agcgcattgc cgttaattca ggcttattgt
    13681 gataaccttt ggcaactcct tcgccaccga tatacaactc gccggtaact ccagtcgcaa
    13741 caagctgctg attttcatcc aatacataca gttgcgtatt cagaatcggt cggccaatcg
    13801 gtggcggcgt gtctccaacc tctttcagtt cctgcgcagt ggaccagatt gtggtttctg
    13861 ttgggccata gaactgtgtt acacgcgcag ccatgctttt aagccgtgca gccagttcag
    13921 cgctgagtgc ttctcctcca accagtgcat ggacgtgctc gatcttcgtc ttcgagttgg
    13981 ccagaagaat tcgccacagc gagggagtgg cctgcatatg ggtaacaccg ctgcgctgca
    14041 ccagccttgc caatacgggc ggattgcgaa gcgcatcgct gctgcccatg accacgcagg
    14101 cacccacagt caacggcaga taaagctcaa gcccggcaat atcaaagata agagtcgtaa
    14161 tcgccagaaa ccggtcgctc gccttcggct ttaattcaac ctgcatgccc tcaagaaaat
    14221 tccataggtt tctgtgtgtg acctcgacgc ctttcggctt tcctgttgat cccgatgtgt
    14281 acagcacata aacaacgtca tcgggcgagc ttaggtcagc agccggtgga gtatcgctga
    14341 atgctgtatc cggcaattca ggcagcagcc ggagaaaatc gccgcttgcc aggcgcatat
    14401 ggacctgggc ttgtgcaatc agcgccgccg gcgatgcgtc cgccagcacc agcaacattc
    14461 gttcaatagg accatccaga tcgagcggca gataactggc gcctgctcgc ataataccca
    14521 gcaataccat caataactgc tcgttgcgcg gcaatgctac agctacaatg tctccgggtt
    14581 ttataccgct ggcgagtaaa tggcgcgcat atcgagtact gtactcattc aactgccggt
    14641 aagtaaccac ggcgtcgcca aataccacag cgggagcatc cggcgtcttc tgtgcccatt
    14701 gttcaacaag agttggaaga ctgatatagg gcagcgctct tgccgtattg ttccatgtca
    14761 ccagcaaacg gcggcgctct tcctgtccga gaatatccaa ctgtcgtagc ggtttgtccg
    14821 gatgcgccag caactgctcg acaagaccca tcagtctacg cctgtgctcg tccagttcag
    14881 ccatgctgta taaatctgga tttgcatcca gatcaaagcg gacatccgtc tccgtgccgc
    14941 ggtcatacac aaacaccgta aggtcttccg caccgctatt ggaaagattg tgcgtgatgg
    15001 tctccgctcc atcaaaattg agcctgtagt caaatggctc aatatttacg ccaagccacg
    15061 cgatattctg ctcctgaccg aaaagaccaa gatcgcggcg gagttcttca tatctgtatt
    15121 gctgatggcg caaggcctgc aagaccaccc gcgatacctg cgcaaacagg tctgcggcgg
    15181 tcgtctccgg aataaatgag aggcgcaccg gaaccatatt ggcctctacc cctacggaag
    15241 aacgcaagac aggattgaga cgccctgaga ctggcatccg aaaaaccagg tcctctgcgc
    15301 cagtcatgcg gtgatagtaa gcggcgatcc aaccgatcag gacttgcggc agactggccc
    15361 cagcctgctt gcccaactct gccagttggc ggacaatcgc agccggaaga tggcctgtgc
    15421 tcctctgcag gctgctgctc aattcatgcc ggcaattgcg ccgcgagagc gtcactgctt
    15481 ccggcatccg ggcaaggtgc tcgagccagt attcgcggtc acgccgaaaa cggtctgagc
    15541 tgcggtagcg ggtttcagct tcaatcatct ccctcaccgt gcagaaacgg tttggggccg
    15601 gctcttctcc ctggatatac gctgtgtata cttccgccac acgccgggcc accagtccgc
    15661 cgccataacc atcatgcaca atatgatggg cgcgctgata ccagaaatac cggttgtcat
    15721 ccactttcag cagcgctgaa gtccacaagg gatcgtgagc aaggtccacc ggacggcgca
    15781 gctcgtccct catccacgcc tcgcttgcag cccgtggatc gggttcgcga ctcatgtcaa
    15841 tgtaaggcaa atcgccctca tagcagcttc gcaggacttg cagcggctta ccgtcttctt
    15901 caatcacgga tacgcgtaac tgctctgcct cggagatgac ccgatgaagt gcctgctgaa
    15961 acacttccgg ttttaccggg ccgcatatct ccacggcctc ggcaatattc ataagtgcgt
    16021 ctggatgaat tttctggcta aaccacaggc cgcgttgtgc aactgtcaac ggaaatgagc
    16081 tctggtcgca ctcgatcatt cggttctcca ttcaagtcaa taacaggccg acatccctct
    16141 gtaacataac ggtttacaaa gcacagatat atgcagttat tcagatacag ctgagactta
    16201 gaaagctgag gcacgaaacc gattacttct cttaaagaga agcagtaaca ttactaattt
    16261 gttacaacgt ggactggggg ccttttataa cattgctatt ctgtgcgacc gtagtcttgc
    16321 acacacaatg taaaaaggat gaaatctctg atgaaatgca agttaaatag cgcgctctcc
    16381 cggtccctgc aggccagaac tgccctgcaa aaaccctgac ttcattcaga cgttacgcaa
    16441 ccgggagaac gagaggaaag atattcaatg agtaatttct taatagaaga gatagtacca
    16501 tcattaaaat aaccaggaca atattatttt tacttctata acttgggctt caaaccagtc
    16561 cttccagtgc ccgttccagc ctcacacccc tggaagcctt aagcagcacc acatcgcctg
    16621 cgcgcaggtt tctatccagc caggccccgg ccagttccgg cgattccaga aagatccctt
    16681 ccgtccccgc tttttttgcg ccatctgcaa tgcgttccgc gctgcctcgc actcccagca
    16741 ccaggtctac tccggcggat gcggcggctt ctccgcaact ttcgtgcagg gacgcgccct
    16801 cggaccccag ttccagcatc tcgccggcta tcagaatatg gcgctgtccc ggaattgaca
    16861 tcagcgcctg aatcattgcc ttcagcgctt ccggattgga gttgtagcaa tcattgataa
    16921 tcgttgcgcc gcgcacttgg aacgtctggc cccgtttgtc tccgggaaca agcccggcca
    16981 gcgcagaaac acaatctctt aaaggaactc ctgcttccag accggctgca atcgctgcca
    17041 tcgcgttggc cgcattgtgc tgccccagca attgcaaccg aagatcggct acctgctcac
    17101 cggagcgcac acgaatccgc agtccctccg tccccagttc ttcaatggat tcagcgcgtg
    17161 gatcggcgca aggccccttg ccgaaataga ctgtcttgcc ccgaaagtcc cgcccaaatt
    17221 gcgacacgta agcatcatcg cagttcagaa atgcgacgcc atcggccggc agactggcga
    17281 tcaattcata cttggcgcgg gcgactcctg caattccatc agaaaaattc tccgcatggg
    17341 cattgcccac gttcgtcacg actccccagt ttggcgcagc aatgcgggcc agcgccataa
    17401 tttctcccgc attggacatc cccatctcga tcacagcaat atcgtgctcc tgctcaagcc
    17461 gaagcaattg cagcggaaga ccatatccat tgttaagatt gccctgcgat ttcaaaacac
    17521 gaaatttcgc gccaagcacg gcggcaatcg cttctttcgt tgtcgtcttg cctgcgcttc
    17581 ccgtcactcc caccacaagc ttgccccagt ggcggcgcac cgcagccgcc agcgtctgca
    17641 gggcagtcag aggctcatca acaatgatga gcgcgtgttg ccgtaccgct tccggcagtt
    17701 cgcggagctt gttcttcgag acaacggccg cgcatgcgcc acgatcgagt gctccgccgg
    17761 caaaatcatg agcatcaaag cgctctccac gtactgcaaa gaacaaatcg ccggactgga
    17821 gcgtacgcga atcaatcgaa tatccggtgg aaaaaccgtc tgcgccatgg agcgtaccac
    17881 cagtccatcg cgcaatctgc tcgagagaaa gtttcatgcc ttcgctccca tccgctcgcg
    17941 aatggccttc gctgccactt ctgcatcatc aaaccggatg acacggtcac gcaacgtctg
    18001 tgttttttcg tgtcctttgc ctgcaagcag cacaatgtct cctggtttcg cttcttccac
    18061 cgcaatccga atcgccttcg cgcggtccgc ttctgtcaca aatttgactc cggttctctt
    18121 caggccggca agcgcatctc tgatgatcgc ctccgggtct tcgctgcgcg gattgtctga
    18181 ggtcagcacc acaaagtcgc ttccctcgcc tgctgcgcgt cccatcattg gacgcttcgt
    18241 ccggtcacgg tctcctccac agccgaagag cgtgatcacc cgtccacctt gcagctttac
    18301 aaattcgcgc gccagcctaa tcaggtttcg cagagcatca tccgtatgcg cgtaatccac
    18361 aactaccgta aacggctgcc ctacatccac cgcctggaag cgcccgggca cgcatttgag
    18421 cgatgccgcg cccatcgcaa tctcctcagg agagagtccg cgcgcatatg ccgccgccga
    18481 agctgccaga aggttataca cattcacatg gcccgttagt ttggtttgga tttcccctct
    18541 tcccacagga gtcatcaacg taaaacccat gcctgatgca gacatactta catcttcagc
    18601 ccgaaactcg cccacgccca acccatagga aaaaacctcc gcgccagttt ctctggcttt
    18661 gatggccaat tccgcgccgt aagggtcctc cacattcacg acggccactc gcggaggaag
    18721 gccatttctg ccatcaaaga gcatcgcctt ggccgcaaaa tagttctcca tggtgccgtg
    18781 ataatccaga tggtcccgcg taaggttcgt gaaaattgcc acatcaaatg gaagcgacca
    18841 cactctccct tgctccagcg cgtgcgacga gacctccatc accgcctcgg tcgccccggc
    18901 cttcacgccg tccgctaaca gcgccagcag gtcgcgcgac tccggcgtag tatgcggcga
    18961 ctccacaacc tttcccgcca cgtgatactc aatcgtgccc accaggactg tcttgcgtcc
    19021 tgcgctgttc agcatcgcat ccagcagaaa tgccgtcgtg gtcttgccat tggtgcccgt
    19081 cactccgctc agggcaagct gcttttccgg atgaccaaag aagttcgccg aaacaacagc
    19141 cagggtacgg cgcccatgcg caacttccgt caccgcaatc tgcggatact tctgcaccgt
    19201 ttcatcaaat gccgcagccg tgtccgtcac caccgcgctc gccccacgct caatggccga
    19261 agcaatgtag cggttgccgt ccgtcatacc tccgcgcatg gccacaaaca gcgctcccgg
    19321 cgcaatccgg cgcgaatcat actctatgcc cgtaatttca acctctgggc cggaacagcg
    19381 aacggcctcg acaccacgca atgcttcatg gattttcata aaagattcat ctggttccag
    19441 gccgcaaaca agcttctgcc ctgtttctgc acatcctcaa tattttctaa tttccttccg
    19501 atttccagac aagaccgttc caaaacggga actatggatc ccaatacatc catcctcttc
    19561 gtgttttcct atctggcgct cttcaccgga atcgcactct acctttcccc tgcgcttgtg
    19621 gccatcctca ggacacatcc caaaaggggc agcatctttc tgctgaatct attgctgggc
    19681 tggacgatca tcggctggat tgcggccctg gttttggccc ggaagagctt tgtgaagcct
    19741 ccgccacaaa aagtgaacta caaattattg gtcgagcaag tcgtcttacc gtgtaaaccg
    19801 gactacaact tccgtgccca cggggaccat cgtgccggca gcagggactt gttccctggc
    19861 aattcctgaa ccgagcactt gcacccctaa acctgccgcc tccgcgtttt caatcacgtt
    19921 tctcaccgtt tgcccggtaa acaacggcac ggcgacttgc ttgcccgcat tcacaactac
    19981 cgggcctttt gtttgcggag caggtgacgt cacaaccgcc gcagacatcg gcggggccgg
    20041 tgtctcaaat gcagattttg ccggaagccc gcccatcctc cctcctgatg catcctgctg
    20101 cggcgtcggg gcctgtgcct gcgcaacatc ttttgccacc ggagcggcct cccccggcgc
    20161 ctgctgtggc gcgcgcagcg ggtcatctgc cggaaggtca ttcaccgtgg caaaaagcga
    20221 gttcaggtcg ccattgcttt cttccggatt gtcatcttcc gccaccggag gctggttcct
    20281 ggccgacatc gtttgcggcg tcatctcctg gtcatgcggc acgccaagat attccaggat
    20341 ctgctgggcc aggtcctgaa agaccggtgc gctggcctgc gccccaaagt gcatactgtg
    20401 ctccggcgaa tccatcacaa tcacaactgt aatggcagga ttattcaccg gagcaaagcc
    20461 tacaaacgac gcaacaaatt ttgtcttcga atacgtatgc gtgcgcggat caatcttctg
    20521 tgccgtccct gttttgcctg cggcgctata gccgttcaga tgtaccgcct ggtgcgctgt
    20581 cccaatcgtg accacgtcct gcatcatctt ccgcatctcg gccgctgtca tctctgagat
    20641 gacgcgatgt gcgccatccg gcaatggatc aggcaggctg tttccaggat gaaaggccac
    20701 cggctgtaat ctcgggtcgc ccttcatcag ttccgtgctc ttcagcagaa tatgcggcgg
    20761 cagatacacg ccgccattgg caatggttga aaccatggtg gcaatctgga tcggcgtcac
    20821 cgcgattccc tgccccatgg ggatggcccc catctccgcc gggccccagc gctttaccgg
    20881 ctccagcagt ccgcgtgtct ctgcgggaag ctcgattccg ctgcgctcgc caaatccaaa
    20941 tgcccggata tatttgtaga gcttctccgg gcctatccgc tgccctacat ggatcgcccc
    21001 cacattgctt gaaactgcaa gggccttcca cgccgggacc tgcccgtaat gctcgcccgg
    21061 cgcatcatgc accgtgtgtc ccgcgatgaa aagctgacct ccgccacagt caatcatcgt
    21121 gtccggtgta atcgccttct cctccagtgc cgccgaatat gtcactggct tgaacgtcga
    21181 acccggctca taaacatcgc tcaccgcatg gtccttcagc aggtccgttg tagcgtggcg
    21241 aaaatcattc ggattaaatg taggccggat ggcaagggcc agaatctgcc ccgtatgcgg
    21301 gtcctggacc acgatcgtgc cgctttctga atgcgtcctc tccatgttcc ggtcgagcgc
    21361 ctgctcggcc atgaactgga tgttcgcatc cagcgtgagg acgatattgt ctcccggaag
    21421 cgggtcgcgc tcttcgctgc ccagcacatg acgccgcgca tcaatcgccg tcaacatgcg
    21481 cccgggcgtt ccatgcagtt cgtcatcgaa attctgctcc agtccgccca ggcccgtatc
    21541 atccagccct acatagccga gcacctgcgc tgcaagatca ttgtccggat aaaaccgttt
    21601 gaactctttc tggaagtaaa cacccttcag gttcagcgcc ttcactttgg caataatcga
    21661 aggatcttgc ttgcgcgcca cccatgcgaa attttttgat gcattaaaac gcgccagtat
    21721 ctgctgctct gaggtaaacg aatcggaagc atccgtatgg acaaccttcg ccaatgcggc
    21781 cgccgtggcc gcgcgcgcgt ctcccagttc tgacggaacg gcataaatcg aatccgccag
    21841 caccgtcatc gccagctcat gcagattgcg gtcgtacagt acaccgcgcc gtggagcaac
    21901 ttcgaatgtc cgctgctgct gtctggcggc gcgctctacg aattcatggt gccggatgac
    21961 ctgcaaccac accagacgca acccaatcag ccctgcccat acagcaaaaa ccgccagcgc
    22021 atacaccaga cgcagccgcc ggatgggcgt gaccaccccc ggctcaacag ataaaggtgg
    22081 ctgtgctttt gctttttttc cggactggaa cgagaactgc ttcaaggcgt gctcagtttt
    22141 atttcacact atacaaacta aatcaggtgc cccatacacc caaaacttcg tcatcctgag
    22201 cgaatcttcc ggtagagaac gtgttaaaaa tgctcaaatc gcgagaagct tacagccccg
    22261 ttcttctttt caggactctg gcatttttcg tatatgtaga gtccgtcatt ctggcggccg
    22321 aagggagaaa gaatctcagt gatgtgaggt ttgggccaat actgctaaga ttttcgagtt
    22381 ttactccctc cgtcctccac caaaatcatc cttgggatgg cacagataag cccgctccta
    22441 ccgcacaggc gcaaccggcg tcatcttcgc catcaccgga gcattcgggt ctgccatacc
    22501 gtccggacgt accacctgtc ccggtgccgg ggcctgcagc ccatactgcc gcgcgatttt
    22561 gtcaatgcgt tccggagaag caagctgcgc ttccgtcaga cgaagctggc ggttctgttc
    22621 ctctaactgc tgcacctgct gcttttccgc ttcaacgcga taaccgtatt caatcgctga
    22681 gaagtgctgc catccataga ccatcaccaa cagaagcagc gtcgccatgg cacaggcaaa
    22741 actgcgcatt tccttcacgc gctgcgggtc ggccgccttc accagccgcg aattgtcgaa
    22801 atactttgca aagaaaacct ccggcgtcgg cccccggcgc gcccgctgct gctgcgccat
    22861 aagagacagg ttccgctcgg cggccacggc ctcaatgccc ttgcgtccgc ctgtgatgac
    22921 tgcatgtgtt gcgatgacct gtgtcgccat ttgtgtctcc tgccagcctc tggctactag
    22981 ctctggctct ttcttcaaaa aatttttccc tcttgctgac agctaatagc tggtagctgt
    23041 cagctaaaag ctaccccctc gaacgaccgg gcctgggtcg ctatttctga ggaggaacac
    23101 aactgaagtc gtcttacttt cgccttgtac tacggtggga cgaccccggc ccgatccctt
    23161 tccatcagca gtctccagtt gccagtccct ggctattcag aaactgggga cttataacta
    23221 acaactgtct tcctctccgc tgcccgcagc ttcgcgctgc gtgcccgcgg attgcggtcc
    23281 gtctcctccg cttcggccgt caccggcttc cgcgtcagta cctcgtaaac tccctgccgc
    23341 gccccttcgc gcagcgcatc cttcaccagc cggtcttcga gagagtgaaa gctgatcgcc
    23401 accagccttc cgccccgctt cagcagccgc ggcgccgctt ccagcagtgc tcgtatctcg
    23461 tccagctcgg cattcacata aattcggagg gcctgaaagg tccgtgtcgc cggatgaatt
    23521 cgctcggatt tcatcgccgg ggccgcggcc gccacaactc tggctaattg cgccgtcgtc
    23581 gtcaccggcc gcgccctgac aatggctctg gcgattctcc gcgacctcct ttcctctccg
    23641 aattcgtaaa tcaggtcggc aagctctttt tcgccgatct ggtttaccac ctgttcggcg
    23701 gtgaggccct gacgcggatc catccgcata tccaaaggcc cttccgcctg aaaactgaat
    23761 cccctgtgcg cctcgtcaaa ctgcatcgag ctgacgccaa agtccgccag cagcccatcc
    23821 aggctttctg gcccgatcaa atgggccgcc tcggaaaagg ccacatcgtg caggacaacc
    23881 ttcggcatcg ccggccccag cgtctcgcgt aatgcatcca gccgcccgcg cgccagcgcc
    23941 atcgcctccg gatcgcggtc gaacgcaatc agcgtgccca atggcccaat ctgccgcgca
    24001 atcgccgtcg aatgtcctgc cagccccagc gtcgcgtcac aatacgttcc acccggccgc
    24061 acattcagat atcggatcgc ttctttaaaa agaaccggca catgccgttc acgttccgtc
    24121 atgtgctccc cctcaggggt ttgcattccc tacaaaccaa aacttgccag cgtctgcgcg
    24181 tcgtcctcag tcaacggctc ggtcgccagc ttatgctcaa agctctcgcg attggtcact
    24241 tccaggtacg tctgcatccc gaacaccacc acatcgcctt ccaccttcgc cgtctgacgc
    24301 aggatctgcg gaatcagaac tcgcccctgc tggtccatct ctgccatctg gccgtagtag
    24361 ttcacccggt ccagcagctt cttcttcgcg ggattaaaac tcggaatcgc cgcaatcttc
    24421 tgctcgatct tttcccactc ctgcagggga taaatctcag ccaccctgcc gtccttgctc
    24481 gtgatgtaaa actgggtccc gtaactttct tctatccggc gcttaaactc cgccgggagc
    24541 ttcaaacggc ccttctcatc aacccgagct ggatggttac cccgaaacat ctcattgccc
    24601 ttatcggcga aaagcgtaga atttcgatgt gtctgtgcca aaattcatac gctttagacc
    24661 actttgaacc actttggccc actcacatcg taacccagta gatgctgcgc tcaaagcatg
    24721 ttttgctgtg gagattcaga tttttcgggc tggaaatgat aaggaacagc agcgcagggc
    24781 gaaaatccaa ttactcagg
//
LOCUS       DASRBV010000130        11738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_107363, whole genome shotgun sequence.
ACCESSION   DASRBV010000130 DASRBV010000000
VERSION     DASRBV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107363"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..2248
                     /locus_tag="VFA02_05030"
     CDS             59..2248
                     /locus_tag="VFA02_05030"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HZU09245.1"
                     /translation="MSTTYTYPGVYIQELPSPVHTIAAVATSITAFVGYTARGIDNRA
                     EQLFSFSDYERLFGGLASDSELSYAVQQFFQNGGAEAWVVRVPKHGATGAQVVFSNMT
                     FTALSSGAWANGKVLLDVDYQGVNQTSDATAFNLTITDLTTNTVEYFPNLSLNPAKSN
                     YVVAVINDPDNGSQIVNVAMKSPAPTTPPAVTGITGNALSIPAVLASASNTIALPDKA
                     GVKQNQTQVNATTGDVQSLRVGQYVTFSGDPGTPYQVTGVSPGAGTFTVSPAYGKADD
                     ANSTVTLLAGTANADFTLVLTTTKPAPTAPLPLTITVVPKNSTIPLTVAGLAAIVQQR
                     INSALALQMPGASAVCSAIANGTGQALRLSVLLPQTPDAIVSISGGTGANLLGFPGTA
                     NNAHYAQGTGNSFGNQTSSTTGSDGTDLPYTADIIGDELSFTGLYALNRVDLFNLLSI
                     PDATRAATGDPSSLDPNIDPNAIYSAAIALCDQRRAFLLVDAPPNVSTVQSAVDWKTT
                     HLAVSDANGAAFFPRLRLPDPLNNYQLRTFAPSGVVAGVYARIDSTRGVWKAPAGTEA
                     TLNGVQSMVYKLTDPENGVLNPLGLNCFRTFPVYGSVLWGARTLVGADAQTSQWKYVP
                     VRRMALFLEESLYRGTKWVVFEPNDEPLWAAIRLNVGAFMHDYFRKGAFQGQTPDEAY
                     FVKCDSETTTQSDIDKGVVNILVGFAPLKPAEFVVIQIEQLAGQLQS"
     gene            2284..2811
                     /locus_tag="VFA02_05035"
     CDS             2284..2811
                     /locus_tag="VFA02_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZU09246.1"
                     /translation="MAEFVVNAQRFDPYKNFKFRVKWDGQYVAGISKVSSLKRTTEVV
                     KHREGGDPSTSRKSPGRTEFDAITLERGVTHDPTFEAWAAKVWQLGAGLGSEVSLKDF
                     RKDLILDVYNEAGQLAISYKIYRAWVSEYQALPDLDANANAVAIQHIKLENEGWERDT
                     SVTEPSEPSFTSTRA"
     gene            2827..3513
                     /locus_tag="VFA02_05040"
     CDS             2827..3513
                     /locus_tag="VFA02_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09247.1"
                     /translation="MHALTGERLLSAWDQGMTEHDIERALTMLALAMPEASREHLADL
                     PITERNKLLLRLRALTFGPLLKGFAICSGCETPLEFTLRVDELLTHAEQLKAETVQHS
                     HLRAVNSHDLLAILAAQDAARAEDLLLTRCLVSGGPEVLSFPAIREEFETLNAAAEMM
                     FALDCPNCSRREMADFDIARFLWAETRRAAARLLSEIHELASAYGWSEEAIAAMSAQR
                     RSAYMEMLNA"
     gene            3837..4220
                     /locus_tag="VFA02_05045"
     CDS             3837..4220
                     /locus_tag="VFA02_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09248.1"
                     /translation="MSEDSDQKADNPKQLQPPTSDVRPLKIHASPSPVSLKEFVPEEG
                     PAPALFLKQRKREPHATSSPGRYGPTAKAIEQNAPGSRAPEITISIGHIELRAAHAPQ
                     ATPKPAFRPQLSLDEFLNKKPGVRP"
     gene            4217..5464
                     /locus_tag="VFA02_05050"
     CDS             4217..5464
                     /locus_tag="VFA02_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HZU09249.1"
                     /translation="MSDYLAVGGVSAVLRSLLTTALTTGGPSSLLGAPLSITATSPDL
                     VATGTNEIPRLNLFMYYASPNAAYRNFDLPAYDSAGRRASNPPLALDLHYLLTAYGST
                     QFDPEILLGWGMQVFHEMPIVPREIVQDALTALGTATPENKLLSASTLANQVESLRIT
                     PEALSNDDISRLWMAFQTNYRPTASYLVTVVLIQETQAVKTNLPVQTRTLVALPLQPP
                     VIDSISPATVAPGEMLTIAGSNFVGDVVADTLVAFDQGDPVAPDTIQGNLIRVKVPGT
                     LPAGGRTVRILRQVSFGEPTDPHKGFSSSLAAFQLVPTISSPPASVAAGSTLTLTIVP
                     PVGNTQKAILYLGDFAVPIDERSATGPATSSTLDFPIPPGCAAGNYPLRISIDGADSR
                     LTLDTTQGSPTFGQFLPQIKVTP"
     gene            5461..7380
                     /locus_tag="VFA02_05055"
     CDS             5461..7380
                     /locus_tag="VFA02_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09250.1"
                     /translation="MTTIAPEMQIASLPDSLDRIYRTITRNLSDALRAEAHLPEAAPE
                     TGPCLALERMEYAFQITPFERDILLLCAGASLESRFAKACAAIHNDTHATWPTFGLAL
                     AMLDDPHWSAISRTRPLRYWKLIEVSANHTLLNAPLRIDERVLQFLMDVPTSDEHLEA
                     MVRPLPSVAGERRRYADVIYHAARHWSSPQQGKLHPVLLVGGHESDRQAVFEELCYAT
                     GLRPYTLDAAALPTSPCDLEPLARLWTREAVLTGAGLYITTASLENTCSLSVFLDSLR
                     TPVAVEAAHGSPIEHIEGVRVHVPTLDASGRKALWAESLGPLAAQMNGQLDRLAEYFQ
                     FDEQSIRAAAVHARDANDPDPARATWRICREHARRSLDLLATRIEPRAKWQDLILPAT
                     QMETLRQLAAHVRQRAVVHEAWGFADKHARGLGLSVLFAGASGTGKTMAAEVLAGELD
                     LDLYQIDLSAVVSKYIGETEKNLRRIFMAAEKSGSVLLFDEADALFGKRSEVRDSHDR
                     YANLEISYLLQQMESYRGIAILTTNMQHALDPAFLRRIRFLVQFPFPDATARRQIWER
                     IFPAATPAGTLNFDQLAQLNVSGGAIRNIAIHAAFLAAEDSSPVEMRHLLAAARTEYQ
                     KMEKPLTTAETRGWV"
     gene            7377..7685
                     /locus_tag="VFA02_05060"
     CDS             7377..7685
                     /locus_tag="VFA02_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09251.1"
                     /translation="MRMQTAATLHAPQIQRMPKINFRIERLTLENYSHADQVRFTRTL
                     EKRLRNYAQTVRLNWSAVKSTGRIDAGTLPAGATPEQAARQVARQIVMNLREEENFHA
                     "
     gene            7678..8280
                     /locus_tag="VFA02_05065"
     CDS             7678..8280
                     /locus_tag="VFA02_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09252.1"
                     /translation="MPDISPILRKGAILSLDPTTGVPLGKILLQYNPDSITRSLKPQS
                     VGDQPDRTEILRLKGPPIETIKCDAEIDATDLLAQGDANTIAFGIQPQLSALELLVYP
                     SSAVLIANEVLSFLGTIEILPMESALTLFAWSKNRITPVRITDIEVTEEAFDPQLNPI
                     RAKVTLNMRVLNVNDVGFLTPAGALYMAYQISQETMARLS"
     gene            8297..8662
                     /locus_tag="VFA02_05070"
     CDS             8297..8662
                     /locus_tag="VFA02_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZU09253.1"
                     /translation="MNTALASLIQSAAGNGATTNNNSRYYGAAVQTYTLANGTPVKYL
                     ARRIIPQPDIYASVQNYAVADGDRLDNLAAKFLGDPTLWWMICDANGVTDPDELTAQA
                     GRTILIPVAAGIPAGARNG"
     gene            8655..9791
                     /locus_tag="VFA02_05075"
     CDS             8655..9791
                     /locus_tag="VFA02_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09254.1"
                     /translation="MASGLYLTLLAGPMVPFPVPKPLIDALTSVEIEVSATGRSGFQL
                     TFTLADRSILQTFFLLTANSPIPLLRVVLVVTLGGLPQAIMDGIVEHHEVVPDAMHGS
                     SKLIVSGHDLSAMMDLIDLTGLPFPGMTPDVRVLTILSKYAAFGIIPEVVPVITPDIE
                     VPVEKIPTQKGTDFSYINQLAQEAGYVFYIQPGPVPGTSQAYWGPQVKAGVPQPSLNV
                     NLDAWTNVENLTFHYQPKDAVLPIVFIQEPITKATIPIPIPPITPLNPPLGLAVAVPQ
                     KIVPMEDTAKATPAQALMRGMAKASDAADIVTGEGSLDVLRYGQILNARSLVGVRGAG
                     LAFDGLHYVNSTKHHIKPGEYKQDFTLRRNALISNLPLVPTMPF"
     gene            9801..10307
                     /locus_tag="VFA02_05080"
     CDS             9801..10307
                     /locus_tag="VFA02_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HZU09255.1"
                     /translation="MAKYYGKYRGTVLDNVDPMQMGRLMVQVPDVSNVLPSTWAMPCL
                     PFAGIQAGMYAVPAIGSGVWVEFEQGDIDYPIWVGCFWGSAAEVPALALAGPPVLQQI
                     VIQTTGQNTLMISDVPGPTGGILLKSATGALISITDTGITISNGQGATIAMNGPAVTV
                     NEGALEVI"
     gene            10321..10638
                     /locus_tag="VFA02_05085"
     CDS             10321..10638
                     /locus_tag="VFA02_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09256.1"
                     /translation="MPGFLIQQGATVMCSHGGQAQPTVPNASVTIDGMPSLLVTSPWV
                     VAGCPLVPPPIPPCVTAQWITGTTRVTSFGQPLLVQSGTSICAPTGTPLVPVVTQTRV
                     SAM"
     gene            10646..11011
                     /locus_tag="VFA02_05090"
     CDS             10646..11011
                     /locus_tag="VFA02_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HZU09257.1"
                     /translation="MNLAFPYQFDAGGRTAQTDTASHIRDMIEQLLFTSPGERVNRPT
                     FGTGTAQLVFDPNSDVLAAAQQQVIQASLQQWLSDLIRVQSVTVTAEDATLLVTVQYI
                     IIQSQQQQTQQFVYGGGAQ"
     gene            11008..>11738
                     /locus_tag="VFA02_05095"
     CDS             11008..>11738
                     /locus_tag="VFA02_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HZU09258.1"
                     /translation="MIYFCSQKDRRALVLQSPSLNGIDYLEVIGSPCGNQLALTMLKD
                     ARTLPLAPSQISISGGPDSAVLSVVSVATGTDAQPNVVTITLNQTGDFSAYTLSLVAF
                     PGAIEPPAGLDPALSTVTFSFKAGCPSAADCAPDNCCAPVVRPAPDINYLAKDYDGFR
                     QVMLDRIATLVPGWSESHVPDIGIAMIETLAYAADHLSYQQDAVSTEAYIGTARSRIS
                     LRRHARLVDYYLNEGCNARAWVALSV"
BASE COUNT         2621 a         3386 c         3243 g         2488 t
ORIGIN      
        1 ccccctcacc ctgcggcaag cgatcacaaa caaacaacgg tgagaaaggc cggtgcttat
       61 gtccacgaca tatacttacc ccggtgtcta tattcaggag ctgcctagtc ctgtccacac
      121 gatcgcagca gtagccacgt ccatcactgc atttgtgggc tacactgcgc gcggcattga
      181 caatcgcgca gaacaactct tcagcttctc tgactatgag cggctctttg gcggactcgc
      241 ttcagacagc gaactcagct atgccgtaca gcaatttttc cagaacggcg gcgcggaagc
      301 ctgggtggta cgcgtgccaa aacatggggc cacgggcgca caggtggtct tcagcaacat
      361 gacatttacc gcattgagca gcggcgcatg ggcaaatggc aaagtcctgc ttgacgtgga
      421 ttatcaggga gtaaaccaga catcggacgc aacggccttc aacctgacca ttaccgatct
      481 gaccacgaat acggttgagt actttcccaa tctctctctg aatccggcca aaagcaatta
      541 cgttgttgcg gtcatcaatg atccggacaa tggctcgcaa attgtaaatg tcgcgatgaa
      601 gtctccggcg ccgacaactc cgcctgctgt tacaggaatc acaggcaatg cactgtctat
      661 tcccgcagtg ctggcgtctg cgagcaacac aatcgccctg ccggacaaag caggcgttaa
      721 gcagaaccag acacaggtga atgctactac tggcgatgtc cagtcattgc gtgtggggca
      781 gtacgtgact tttagcggag atccggggac cccatatcag gtgacgggtg tttctcctgg
      841 cgccgggact ttcacagtat ctccggccta tggcaaggca gatgacgcaa actcgacggt
      901 caccctgctg gccggcacgg caaatgctga tttcactctt gttctcacta cgaccaaacc
      961 agctccaact gcgccattgc ctttgaccat tacagttgtt ccaaagaaca gcacaattcc
     1021 gctaaccgta gccgggttgg cagcaatcgt ccagcaacga atcaattccg cgctcgcgtt
     1081 gcagatgccg ggcgcctccg ccgtgtgcag tgccattgcc aacggtaccg ggcaggcgct
     1141 gcgtctgagc gtgctgctgc cgcaaactcc tgatgcgatt gtgagcatct caggcggcac
     1201 gggggcaaac ctgcttggtt ttcccggcac agccaacaat gcccactatg cgcaaggcac
     1261 aggaaattcc tttggaaacc agaccagttc gactacaggc agcgatggaa cagatttgcc
     1321 ttacaccgcg gacatcattg gcgatgaact cagcttcact ggtctgtatg cgttgaatcg
     1381 cgtagacctg ttcaatctgc tgagcattcc cgatgcaaca cgcgccgcaa ccggtgatcc
     1441 atcttcgctt gatcccaata ttgaccctaa cgctatttat tctgccgcca tcgcgctatg
     1501 cgatcagcgc cgtgcattcc ttctggtgga tgctccgcca aacgtcagca cggtgcagtc
     1561 tgctgtggac tggaagacga cccatcttgc ggtttcagat gcgaacggcg cggccttctt
     1621 cccccggttg cggttgcctg atcctttgaa caattatcaa ttgcgtacgt ttgcgccatc
     1681 cggagtagtc gcaggagtct acgccaggat tgacagcacg cgcggcgtgt ggaaagcgcc
     1741 tgccggcacc gaggccacac tgaatggcgt gcagagcatg gtctacaagc tcaccgatcc
     1801 tgagaatggg gtcttgaatc ctctcggact gaactgcttc cgcacgttcc ctgtgtatgg
     1861 ctctgtcctt tggggagcac gcacactggt gggcgcagac gcgcagacca gccagtggaa
     1921 atacgttcca gtgcggcgca tggcgctctt tctggaagaa agcctctacc gcggaacgaa
     1981 gtgggtggtc tttgaaccca acgatgagcc gctgtgggcg gccatccggt tgaacgtagg
     2041 cgcatttatg catgattact ttcgcaaagg cgccttccag ggacaaaccc ccgatgaggc
     2101 ctacttcgta aaatgcgaca gcgagaccac cacgcagtct gacatagaca aaggtgtggt
     2161 gaacattctg gtaggctttg cgcctctcaa accggcggag tttgtcgtga tccagattga
     2221 gcagctggcc ggccagctcc aatcgtaagc aaaaacagcg gccatctaac tgaggaggaa
     2281 gccatggcag agtttgtcgt caatgcacag cgttttgatc cgtataagaa ctttaaattc
     2341 cgcgtgaaat gggacgggca gtatgtggcc ggcataagca aggtgtcttc tttaaagcgc
     2401 accaccgaag tggtaaagca ccgcgaaggc ggcgacccca gcaccagccg caaatcgccc
     2461 ggacgcacgg agtttgacgc cattaccctt gagcgcggtg ttacacacga tcccaccttt
     2521 gaagcctggg cggccaaagt atggcaactg ggcgcagggc ttgggtcgga agtctctctg
     2581 aaagactttc gcaaagacct gattctcgat gtgtataacg aggccggtca gcttgccatc
     2641 agttacaaaa tatatcgcgc ctgggtatcg gaatatcagg ctttgccgga ccttgatgca
     2701 aacgcgaacg cagtcgccat tcagcacatc aagctggaaa acgagggctg ggaacgcgac
     2761 acgtctgtga ccgagccaag cgaaccgagc ttcacttcca cgcgggcata agcaagggca
     2821 agtaccatgc acgcgcttac cggcgaacga ttgctttcgg cctgggacca gggcatgacg
     2881 gagcacgaca tagagcgtgc tttgaccatg ctcgcgctcg ccatgcctga ggccagccgc
     2941 gagcacctgg ccgatctgcc catcacagag cggaacaagc tgctgctccg gcttcgtgcg
     3001 cttactttcg gccctttgct gaaagggttt gccatctgct caggctgcga aacaccgctt
     3061 gagtttacat tgcgcgtaga cgaattactg acgcacgcag agcaacttaa agcagagact
     3121 gtgcagcaca gccatctgcg ggcagtcaac agccatgatc tgttggcaat actcgcagcg
     3181 caggatgccg cacgcgcaga ggacctgctg cttacccgct gcctggtttc cggtgggcct
     3241 gaggtccttt cattccctgc cattcgcgaa gaatttgaaa cattgaatgc ggcagcggaa
     3301 atgatgttcg cactcgattg tccaaactgc tccagacgcg aaatggcaga tttcgatatc
     3361 gcgcgctttc tctgggctga gacgcgacgc gcggcagcgc gcctgctcag tgaaatccat
     3421 gaactggcca gcgcctatgg atggagcgag gaagccatcg ccgctatgag cgcacagcgg
     3481 cgcagcgcat atatggagat gctgaacgca tgagcgactt attgcaacgc atcgtgcagc
     3541 ggacactggc accttcggca gtgattcagc caatccctgt ctctcgcttt gaacctgcgg
     3601 gaatttctgg gggcactttt cagattgagg aagatgcacc agcgactacg tatccgcaac
     3661 agttacatat gcagaaaaca atctcaggtg aagcgccttt ggagatacaa caatcacaca
     3721 acgtgacaaa agcagaacat ctggcaccat ctccaaatcc ttctagcgaa aaggcaggaa
     3781 cgccgagtca ggtttcacta cgacaaaaaa cacggcaatc tgtggcgcgg gacattttga
     3841 gcgaagattc cgaccagaag gcagacaacc cgaaacagtt gcaaccgccc acttccgatg
     3901 ttcggcctct gaaaatacac gcaagcccat cacctgtttc gttgaaagaa tttgtgccgg
     3961 aagaaggacc tgctccagca ttgtttctga agcagcgcaa acgcgaaccc catgcaactt
     4021 catcgcctgg gcgatatggg ccaactgcca aagccatcga gcaaaatgcg cccggctccc
     4081 gcgcccctga gatcactatc tccataggtc acatcgaact acgggctgca cacgcgccgc
     4141 aagcaacgcc caagcctgca ttccgtccac agttgtctct cgatgaattt ctcaacaaaa
     4201 agcccggagt gcggccatga gcgactacct ggcagttgga ggagtgagcg cagtgctgcg
     4261 gtcgctgctg acaactgcgc tgacgacggg aggaccgtcg tcccttctcg gcgcgccgct
     4321 gagcatcacc gcgacttcgc ccgaccttgt tgcgacaggt acaaatgaga ttccgcgact
     4381 gaacctcttc atgtattacg caagcccgaa cgcagcgtat cgcaatttcg acctgcctgc
     4441 ctatgattcc gcaggccgtc gcgccagcaa tccgccgctt gcgcttgatt tgcattatct
     4501 gctcacagcg tatggcagca cacagttcga cccggaaatt ctgctgggat gggggatgca
     4561 ggtctttcat gagatgccca ttgtgccgcg cgaaattgtg caggatgcgt tgactgcact
     4621 tggcaccgca acaccggaaa acaagctgct ctctgcaagc acgctggcca atcaggtgga
     4681 atcgctgcgc attacgcccg aggccctctc gaacgatgac atcagccgcc tgtggatggc
     4741 ctttcagacg aactatcgcc cgacggcttc gtatctggtc acagtggttc tgattcagga
     4801 aacgcaggcg gtgaagacaa atttgcctgt ccaaacgcgc acgctggttg cgctaccctt
     4861 gcagcctcca gtgattgaca gtatttcgcc cgcgacagtt gcgccgggag aaatgctgac
     4921 gattgcagga agcaatttcg ttggcgatgt tgtagctgat accctggttg ctttcgatca
     4981 gggtgatcct gttgccccag acaccatcca gggcaatctg attcgcgtca aggtccctgg
     5041 cactcttccg gctggcggcc gcacggtccg cattttgcgg caggtgagct ttggcgaacc
     5101 gactgatccg cataagggat tttcctccag tctggctgcg tttcaactag tgcccacgat
     5161 cagttctcca cctgcgagtg tggccgccgg atctacgctt acgctgacaa ttgttcctcc
     5221 agtgggcaac acgcagaagg caatcctgta cctgggcgat tttgcggtcc cgattgatga
     5281 acgctctgca accggtccag ctacttcttc aacgctggac ttccccattc cgccgggatg
     5341 tgctgctggg aattacccac tgcgtataag cattgatgga gcagacagca ggctcacgct
     5401 cgacacaacg caaggcagcc cgaccttcgg gcaatttctg ccgcagatca aggtgactcc
     5461 atgactacga ttgcgccgga aatgcaaatc gcttctcttc cagattcgct cgaccggatc
     5521 tacaggacga ttacgcgcaa tctcagcgat gcattgcgtg cggaagcgca tttgcctgaa
     5581 gcagcgccgg aaactggacc ctgccttgcg ctggaacgga tggaatatgc atttcagatc
     5641 actccgtttg agcgcgatat tttgctgctt tgcgcgggag caagtcttga aagccgcttc
     5701 gcgaaggcct gcgcagctat tcacaacgac acgcacgcta cctggcccac gtttggattg
     5761 gcgctggcga tgctcgacga tccacactgg agcgccatca gccgtacacg tccgctgcgt
     5821 tactggaagc tgattgaagt ctctgcgaac catactttgc tgaacgcgcc gctgcgcata
     5881 gatgagcgtg tactacagtt cctgatggac gtgcctacca gcgatgagca tctggaagcg
     5941 atggtccgtc cattgccatc tgtggcaggc gaacggcggc gttatgcgga cgtaatctat
     6001 cacgcggcaa ggcactggag cagtccgcaa cagggcaaac tgcatcctgt tctcctggta
     6061 ggagggcatg aaagcgatcg tcaagctgta tttgaagagc tttgctatgc aacggggctg
     6121 cgcccataca ctctggatgc tgctgcgctt cccacctcgc cttgcgatct ggagccattg
     6181 gcgcggctgt ggacccgtga ggctgttcta acgggcgctg gtctgtatat cacaacggct
     6241 tcgcttgaaa acacgtgcag tctgagtgtt tttctcgatt cgctgcggac accagtggct
     6301 gtggaggcag cacacggttc acctatcgag catattgaag gtgtgcgcgt gcacgtgcca
     6361 acgctcgatg ccagcggacg aaaggccttg tgggcagaat cgctgggccc tcttgcagca
     6421 cagatgaacg gtcagctcga caggctggcc gaatacttcc agtttgatga gcagtccata
     6481 cgcgccgctg cggtccatgc gcgcgatgca aatgaccctg accccgccag ggcgacatgg
     6541 cgcatctgcc gcgaacatgc gcgccgctcc ctggaccttc ttgcgacgcg tattgagccg
     6601 cgcgcgaagt ggcaagacct tatcctgcct gccacacaaa tggaaacact gcggcaactt
     6661 gccgcgcacg tgcggcagcg cgctgttgtg catgaggcct gggggttcgc agacaaacat
     6721 gcgcgtggtc tcggcctgag tgttctgttc gcgggcgcaa gcggcacggg caaaacgatg
     6781 gctgcggagg tccttgccgg cgagcttgat ctcgatctct accagattga cctttccgca
     6841 gtcgttagca aatacattgg cgaaacggag aaaaacctcc gccgcatctt tatggcggca
     6901 gagaagagcg gctctgttct gctgtttgac gaagctgacg cgctgtttgg aaagcgcagc
     6961 gaagtacgtg acagccacga ccgttacgcg aaccttgaaa tcagctattt gttgcagcag
     7021 atggagtcgt atcgcggtat cgcgattctt actacaaaca tgcagcatgc gttggatccg
     7081 gcattcctgc ggcgaattcg ctttcttgtg cagtttccat ttccggatgc gacagcacgg
     7141 cggcagatat gggagcgcat tttccctgct gcaacgccgg ccggcacact caatttcgac
     7201 caactggccc agttaaatgt atcgggcggg gcgatccgca acattgccat ccatgctgct
     7261 tttcttgcag cagaagacag ttcgccggtg gaaatgcgac atcttctggc cgccgcgcgc
     7321 acggaatatc aaaagatgga aaagccgctg accacagcag aaacgcgagg ctgggtatga
     7381 ggatgcagac agcagccaca ctccatgcgc cacaaataca gcgcatgcca aagatcaact
     7441 tccgcatcga gcggcttacg cttgaaaact attcgcacgc agaccaggtc cggttcacca
     7501 gaacactcga gaaaaggctg cgcaactacg cacagactgt ccggcttaac tggtcagcag
     7561 taaaaagcac aggacgcatt gatgcaggaa ctctgcccgc gggggccaca ccagagcagg
     7621 cagcacgcca ggtcgctcgg caaatcgtta tgaatctgcg cgaagaggag aacttccatg
     7681 cctgatattt ctcccattct ccgcaaaggt gcgattctct cgctcgatcc aactacgggg
     7741 gtgccgctgg gaaagatcct gctgcaatac aatcctgatt ccatcacgcg atcgctcaaa
     7801 ccgcaaagtg taggagacca gccggaccgc acggaaatcc tgcgactgaa gggccctccg
     7861 attgaaacga tcaaatgcga cgcagaaatt gatgcaaccg accttctggc gcagggcgat
     7921 gccaacacga tcgcgttcgg cattcagccg cagctttcgg cgcttgagct gctggtgtat
     7981 ccgtcaagcg cggtactgat tgcaaacgag gtcctttcat tcctgggtac gattgagatt
     8041 ctgccgatgg aatctgcgct tacgctcttt gcctggagca agaaccgcat cactcctgtt
     8101 cgcattaccg acattgaagt gacagaggaa gcattcgatc cacaactcaa tcctatacgc
     8161 gcgaaagtca cactcaacat gcgtgtgctg aatgtgaacg atgtcggctt tctgactccg
     8221 gcaggcgcgc tttacatggc ttaccagatc agccaggaaa caatggcccg gttgtcgtga
     8281 caaggggaga tggaagatga acaccgcgct tgcatcgttg atacagtccg ccgctggaaa
     8341 tggcgctacg acaaacaaca acagccgcta ttacggtgct gcggtgcaga catacacgct
     8401 tgcaaacggc accccggtca aataccttgc gcgccgcatc attccgcagc ctgatattta
     8461 cgcatccgtg cagaattacg ctgttgcaga cggagaccgt ctcgacaacc tggctgcaaa
     8521 attcctggga gatccgactc tctggtggat gatctgtgat gctaacggcg taactgatcc
     8581 tgatgaactg acggcgcagg caggccgcac gattttgatt ccggtggccg caggcattcc
     8641 tgctggagcg cgcaatggct agcggactct acctaacgct gctggccgga ccgatggtgc
     8701 cgttcccggt gcccaagcct ctcattgatg cgcttacatc ggtggagatc gaggtgtccg
     8761 cgacgggccg cagtggtttt cagctgacgt ttacgctggc agacaggtct atcctgcaaa
     8821 cattttttct tcttaccgcc aactcgccga ttccattgct acgcgtggtc ctagttgtca
     8881 ctctgggcgg gcttccgcag gccattatgg acggcattgt cgagcaccac gaagttgtgc
     8941 ccgatgctat gcatggctca tcaaagctga ttgtcagcgg acacgacctt tccgccatga
     9001 tggatctgat agacctcacc gggctgccat ttccgggcat gactcctgat gtgcgcgtac
     9061 ttacgatcct cagcaaatat gcggctttcg gcattattcc tgaagtcgtg cccgtgatta
     9121 cgccagacat cgaagttcca gtggagaaga tacctacaca gaaaggcacc gatttctcct
     9181 atatcaatca gcttgcgcag gaggccggat atgtcttcta cattcaacct ggacctgtgc
     9241 cgggaaccag ccaggcgtat tgggggccac aggtaaaagc aggagtgccg cagccttcgc
     9301 tgaatgtgaa tctcgatgcg tggacaaatg ttgaaaacct cacatttcat taccagccaa
     9361 aggatgcagt gctgccgatt gtcttcatcc aggaacccat cacgaaggcc accattccga
     9421 ttccaattcc gccgattacg ccgctgaatc ctccgctggg gctggccgtg gccgttccac
     9481 aaaaaattgt tcccatggaa gacacggcga aagcgactcc ggcacaggcg ttgatgcgcg
     9541 gaatggcgaa ggcctccgat gcggcagaca ttgttacagg tgaaggttcg ctcgacgtac
     9601 tacgttacgg acagatactc aacgcgcgga gcctggtggg cgtgcgcgga gcggggctgg
     9661 ccttcgacgg cctgcattac gtgaacagca caaagcacca cattaagcca ggcgaatata
     9721 agcaggattt tacgctgcga agaaacgcgc tgatttcaaa cctgccgctt gttccaacca
     9781 tgccgtttta ggagaaagga atggcgaagt attacggcaa atatcgcggg acggtgctcg
     9841 acaacgtgga ccccatgcag atgggccggc tgatggtgca ggtgccagac gtgtcgaatg
     9901 tgctgccgtc cacgtgggcc atgccgtgcc tgccatttgc gggtatccag gcaggaatgt
     9961 atgccgtgcc tgcaatcggt tcgggcgtgt gggtggagtt tgagcagggc gatattgatt
    10021 atcccatctg ggtgggctgc ttctggggtt cggcagcgga ggtccctgcg ctggcgctgg
    10081 cagggccacc ggtgttgcag cagatcgtca tccagaccac gggccagaac acgctgatga
    10141 tcagcgatgt tcccgggcct acaggaggca tcctgctgaa gtctgcaacc ggcgcgctga
    10201 tctcgattac agacacgggc atcaccatca gcaatggaca aggcgcaacc attgccatga
    10261 atggtcctgc cgtcaccgtg aacgaaggcg ccctggaagt gatttaaggg aggaagctca
    10321 atgccgggat ttttgattca acaaggcgca accgtgatgt gcagccacgg cggacaggcg
    10381 cagcctacag ttcccaacgc ttccgtaacg atagacggta tgccgtcact actggtaaca
    10441 agcccgtggg tggttgcagg atgtccgctt gttccaccgc cgattccacc gtgcgttacg
    10501 gcgcaatgga tcactggcac aacgcgcgta acttctttcg ggcagccgct gttagtgcag
    10561 agcggtacgt ccatctgtgc gccgacagga acgccgctgg tcccagtcgt gacccagacg
    10621 cgtgtgtccg caatgtgagg caaagatgaa tctggctttc ccgtaccaat tcgacgctgg
    10681 cggccgcaca gcgcagaccg atactgcctc gcacatccgg gacatgattg agcagttgct
    10741 cttcacttcg ccgggtgagc gcgtcaaccg tccgactttt ggcactggaa ccgcacaact
    10801 tgtttttgac ccgaacagtg atgtgcttgc cgccgcgcag cagcaggtga ttcaggcgtc
    10861 actccagcaa tggctttcag atttgattcg cgtgcagtcg gtcactgtca ccgctgaaga
    10921 cgcgacgctg ctggtcaccg tgcaatatat catcatccag tcgcagcagc agcagacaca
    10981 gcagtttgtt tacggcggag gcgcgcaatg atctacttct gctcgcaaaa agaccgccgc
    11041 gcgctcgtgt tgcaaagtcc atcgctcaac ggcattgatt atcttgaagt tattggcagc
    11101 ccatgcggaa accagctcgc cctgaccatg cttaaggatg cgagaactct tccgcttgcg
    11161 ccttcgcaga tttcgatttc gggcggcccg gacagcgctg ttctcagtgt agtttccgtc
    11221 gcaaccggaa cagacgcgca gcccaacgtg gtgaccatca cacttaacca gactggtgat
    11281 ttttctgcct acacgctttc gctcgttgcg tttcccggag ccatcgaacc gcctgcgggg
    11341 cttgatccgg cactttctac cgtgactttt tccttcaaag cagggtgccc aagcgccgca
    11401 gattgtgcac cggacaattg ctgcgcacca gttgtcagac ctgctcctga tatcaactat
    11461 cttgcaaaag actatgacgg ctttcgccag gtgatgctcg accgtatcgc aaccttagtt
    11521 cccggatggt ctgagagcca tgttcccgat atcggaatcg cgatgatcga aacactcgct
    11581 tacgctgctg accacctcag ctaccagcag gacgccgtca gcacggaggc ctatattggc
    11641 acggcgcgca gccgcatctc attacgccgg catgcgcgtc tggtggacta ctatctcaac
    11701 gaaggatgta atgcgcgcgc gtgggttgcg ctttcggt
//
LOCUS       DASRBV010000131         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_158176, whole genome shotgun sequence.
ACCESSION   DASRBV010000131 DASRBV010000000
VERSION     DASRBV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158176"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..622)
                     /locus_tag="VFA02_05100"
     CDS             complement(317..622)
                     /locus_tag="VFA02_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZU09259.1"
                     /translation="MLAGKWKVLIVWHLSFGPRRFSGIRELLPGVSEKVLTAQLRELE
                     RDGVIKRIPDGNTPPRVDYMLSDAGTELIPVMEAMCSWGSKHLGVPPNLVHRPEMRP"
     gene            808..1212
                     /locus_tag="VFA02_05105"
     CDS             808..1212
                     /locus_tag="VFA02_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZU09260.1"
                     /translation="MATKLSLEQMKQRVRDHFEDFVNRRNASVIHKNMTPDFYDHDGP
                     GGRPAGVTKDEQMMLGMYNAMPDLHLTIEEMIAEGDKVVCRNIWRWTDPASGRKMQFH
                     GFVLWRFEGDKIAERWATVTQPAEGTSWTTQA"
     gene            1252..2100
                     /locus_tag="VFA02_05110"
     CDS             1252..2100
                     /locus_tag="VFA02_05110"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU09261.1"
                     /translation="MILITGASGNVGREVLKQVAQTGVKVRAAYQSAGKAAEAPAGVE
                     TAIVDYNQPETLRAALKGVERVFLVGPPTAQVTALERSAMDVIKQSSVRHVIKLSAMG
                     GRDAIFPGLHAESEDYIRSTGVPYTFLRPNGFMQNMIIYNAGTIKAQNAFYGSEGEGR
                     VSQIDIRDVAAVAVKALTEEGHTGKAYTLTGPEALAGSETAKILSEAIGREIRFINLP
                     PEQLRQALLATGMSEWSADALLDLQRYYREGKAATVTNDVEQVVGRKPVRFAQFVHEY
                     KSAFIP"
BASE COUNT          526 a          568 c          585 g          470 t
ORIGIN      
        1 agtcttccgg aaaatagata ttcccttcta ccgtctcgta agtgtcactt tctgcaagca
       61 cttttccgtt ccagactgct tttgccatcc gttcttttca atccttctat gaatcgaatt
      121 cccgtataca ttagcagatg ctggcaccat aacaaaagac gcaaggcggc gttaagttgg
      181 cccacagttg tttctgttga gtaaacagcg atagcagcag tagtcaattt tggaataccg
      241 ggcacttcat tccatgcaca ataaaatcac gtcgcagcaa acaaaaactg cccgcgtatc
      301 ctcgccagct tgcaggctaa ggccgcattt ccgggcgatg cacaaggtta ggtgggactc
      361 ccagatgttt ggatccccaa ctgcacatgg cttccatgac cgggatgagt tcagtcccgg
      421 catcgctcag catgtagtcc acacgaggtg gggtgtttcc gtccggaatg cgctttatga
      481 ccccgtctcg ctccagttcc cgtaactggg cagttagcac cttctcactg actcccggca
      541 acagctcacg aatcccggag aagcggcggg gcccgaagga caaatgccag acaattagta
      601 ccttccactt tcctgcaagc acatttgaag tggcctacac cggacatcca aaatggtttt
      661 tcatctgcct gccgttcttc tggaaatacg ggcctgacac gacggactta caaaattgtg
      721 cgtacttccg gaaatgtccg tcctgggcta tctttgataa cgcatgggaa tcatacgtcg
      781 gagtcatcat aatttcagga gcaaaacatg gcaaccaagc tctctttgga gcagatgaaa
      841 cagcgagtga gagaccactt cgaggatttc gtcaacaggc gcaatgcaag cgtgatccac
      901 aaaaacatga cgcccgattt ctacgatcat gatggccccg gtggtcggcc agccggagtt
      961 accaaagacg agcagatgat gctcggcatg tacaacgcca tgccagacct gcacctgacc
     1021 attgaagaga tgattgctga gggggacaaa gtagtctgcc gtaacatctg gcgctggacc
     1081 gatccggcat ccggcaggaa gatgcaattt cacggttttg tgctctggcg gtttgaggga
     1141 gataaaattg cggagcgctg ggccacggtc acgcagcctg ctgaaggcac atcctggaca
     1201 acgcaggcct gattcattcg aggggcctga aaatcaaata tggagggctg catgattctg
     1261 attacaggag cgagtggaaa tgtcggcaga gaagttctca agcaggtggc acaaacaggt
     1321 gtcaaagtgc gggcagctta ccagtcggcg ggtaaggccg cagaagcacc tgcgggagtt
     1381 gaaaccgcga tcgttgacta caatcagccg gaaacgcttc gagcggcgct taagggcgtg
     1441 gaacgggtgt tcctggtcgg tccgccaact gcgcaggtta ccgcgcttga gcgcagcgcg
     1501 atggacgtca tcaaacaatc cagcgtccgg catgtcataa aactctcggc catgggaggg
     1561 cgcgatgcaa tctttcccgg cctgcatgcc gagtctgaag actacatccg ctctaccggt
     1621 gtgccgtata cgtttttgcg tccgaacgga ttcatgcaga acatgatcat ctacaacgcc
     1681 ggaaccatca aggcgcagaa cgcattttac ggcagtgaag gcgaagggcg cgtcagccag
     1741 atagatattc gtgatgtagc ggcagttgcc gtcaaggcgt taacagaaga gggccacacg
     1801 ggtaaggcat ataccctgac cggtcctgaa gcccttgcgg gcagcgaaac cgcaaaaata
     1861 ctttcggaag ccattggtcg tgaaatccgg ttcatcaacc tgccccctga gcagttacgg
     1921 caggcgttgc tggcaacagg tatgtccgag tggagtgctg atgcgttact tgatctccaa
     1981 cggtattacc gtgagggcaa ggcggcgacc gttaccaatg atgtggaaca agtcgtggga
     2041 cgtaagccgg tccgctttgc gcagtttgtc catgagtaca aatccgcttt tatcccctga
     2101 cgtcatcctg acgctgaccg ggaacgatgg cagccgtcat cacttgtgg
//
LOCUS       DASRBV010000132         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_58102,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000132 DASRBV010000000
VERSION     DASRBV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58102"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="dnaN"
                     /locus_tag="VFA02_05115"
     CDS             complement(<1..601)
                     /gene="dnaN"
                     /locus_tag="VFA02_05115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZU09262.1"
                     /translation="MPGVGVEQEKQTAQGALEISVSRQELLRELTATQSVVERKTTIP
                     ILSNFLLEAENDRLTITGTDLDQSMKTSCAAKVKKPGSCTIPARKLYDYIKLLGDGDI
                     SIKLLENHWVQIRAGRSNTKMVGMARANFPQVPEFPETSLTKIPASSLKNLISKTIFA
                     ISNEESRYTLNGALLVLKAESLTMVATDGHRLAHIEKTGE"
     gene            1255..>2139
                     /locus_tag="VFA02_05120"
     CDS             1255..>2139
                     /locus_tag="VFA02_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZU09263.1"
                     /translation="MLSSIQMDPVTHFLTGAVLARSGFNRKAAYATLAMTLAAEAPDL
                     DVVWSFRGPVAAFEHHRGITHTFLAAPFIALITVAVVWGIHRFRKKPTKAPVRWGLLW
                     CFALIATLSHLLLDFTNNYGLRPFYPFNPRWYSWDIVFIVEPLILIALILALVVPSIL
                     GLADREIGAKRAVFRGRGWAIAALSFMALLYAVRNGEHDHAINLVRNTGVVNGRILRI
                     AAQPYPINPFRWYVITETPDFYQTEWVYTRADRIDTDTDQTIYKPEVTKAVAAAKQSY
                     LGKVYLDWAKFPITEDKGP"
BASE COUNT          446 a          638 c          542 g          513 t
ORIGIN      
        1 tctcgcccgt cttctcaatg tgcgccaggc gatgaccatc cgtcgccacc atcgtcaggc
       61 tctcggcctt cagcaccagc agtgcgccat tcagcgtata gcgcgactct tcgttcgaga
      121 tggcaaaaat tgtttttgag atcaggttct tcagcgagga ggccggtatt ttcgtcagac
      181 tggtctcagg aaactcaggc acctgcggga aattggcgcg cgccatgccc accattttcg
      241 tattggaccg cccagcgcgg atctgtaccc agtggttttc cagcagtttg atcgaaatgt
      301 caccatcgcc cagcagcttg atgtagtcat acagcttgcg cgccggaatg gtgcaggagc
      361 cgggcttttt cacctttgcc gcgcaggatg ttttcatgct ctggtcaagg tcggtcccgg
      421 taatggtgag cctgtcgttt tctgcttcca gcagaaagtt ggaaaggatc gggatcgttg
      481 tctttcgctc gaccacgctc tgcgttgccg tcaattcgcg cagcagttcc tgcctgctga
      541 cgctgatctc caaggcgcct tgcgccgttt gtttttcctg ctctacgcct acgccaggca
      601 taccatttgc cccctttagg gatttgcctc aatttacacc actgcgcgcg gaagaatgaa
      661 atacgcagcc tttggcattc attctagtga taaattgcct caattgtacc tgcggaaaac
      721 gtggtctcgt gtaggaaatc gtgcgcccgg ttttttagga tgccgaatgg gccacggctg
      781 tttctaagaa aaagaaaagt agttttaaac cagcagtagt agtagatgag tgcggaaaag
      841 tggaaagctc gccccaaatc aatccagaca ttggttccgg aaggtacacg ttttagaaaa
      901 cccgtgctat gcaaaaagca aaacttggaa tcctgggttg aaactcccaa tcgcataccg
      961 cgatctccga attcttcaca agcatccgga aaacccaatg tggaaacccg ggcggcagcg
     1021 tggaaaccac gtcctctgcc aggaatgatg cgcgttccgc gacgagcaga attccaccga
     1081 aacccggtta tccaaagatt ccacaactcc tttaccgccc tgagcataaa ccgtcacttt
     1141 gtccttttcg ggccggaatt ttttttagtt gtcatcccgg ccggagcggc attccgtgga
     1201 ttgaggcaat cttttggcac tatctctgcg gagcgatccc atgtttcaag tggcttgcta
     1261 tcatccatcc agatggatcc ggtcacacat ttccttaccg gagcagtcct ggcccgttcg
     1321 ggattcaacc gcaaagccgc ttatgccact ctggccatga cgctcgcggc agaggcgccc
     1381 gatctcgacg tggtctggag ctttcgcggt cccgttgctg cctttgagca ccatcgcggc
     1441 atcacgcata cctttcttgc cgcacccttc atcgcgttga ttacagtcgc ggtcgtctgg
     1501 ggcatccacc gtttccgaaa gaagcccaca aaggccccgg tgcgctgggg cctgctgtgg
     1561 tgctttgccc tgattgctac cctcagccat ctgctgcttg acttcaccaa caattacggc
     1621 ctgcgcccgt tctatccctt taatcccagg tggtattcat gggacatcgt cttcatcgtt
     1681 gagccactta tcctcattgc gctcatcctc gccctggttg tgccctccat cctaggcctg
     1741 gccgaccgcg agattggcgc aaagcgcgcg gtctttcgcg gccgcggatg ggccatcgca
     1801 gcgctcagtt ttatggcgct gttatatgcc gtgcggaacg gcgagcatga ccacgccatc
     1861 aacctggtgc gcaataccgg cgtggtcaat ggacgcattc tgcgtattgc cgcccagcct
     1921 taccccatca acccgttccg ctggtatgtc attacagaga cgccggactt ctaccagacc
     1981 gaatgggtct acacacgcgc tgaccgcata gatacggaca cagaccagac catctacaag
     2041 ccggaagtga caaaggccgt cgccgcagcc aaacagtctt atctgggcaa ggtatacctc
     2101 gactgggcaa agttccccat caccgaagac aaaggcccc
//
LOCUS       DASRBV010000133        10696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_462723, whole genome shotgun sequence.
ACCESSION   DASRBV010000133 DASRBV010000000
VERSION     DASRBV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462723"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFA02_05125"
     CDS             <1..337
                     /locus_tag="VFA02_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HZU09264.1"
                     /translation="EGSMGALVASPQQARFGRAKAVALPSDCQTCDVRFACNGECPKH
                     RFAQTSAGEYGLNYLCAGYKHFFRHIDPYMRFMANELNNHRAPARVMEWARSGRAAVA
                     QSAFSACRQ"
     gene            complement(911..2209)
                     /locus_tag="VFA02_05130"
     CDS             complement(911..2209)
                     /locus_tag="VFA02_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09265.1"
                     /translation="MGLAYAPDFLHGKTVFRGGMGLYYNPFNDYYTPQAYGYSIANTL
                     VSSNDNMLTPAATLSDPFPGASNPLRQPFGSSQGINTYLGQAITFTNPKPRAAYSIRW
                     SFDIQHDLGHNTLVQVGYIGNHQVHMSYTAPLNYFPLNLLSRSAYYDPAVTKTYGSAV
                     TNPFYGLIQGSSIGTGKTMTLSSLFSRFPEYSSVSEQLVPAASSNLNMLVARLTKRLS
                     SGLSANVNYEYSRLLAAASPLNAGGPLWYGTNASDFPQHLAVTATYELPFGRGRAFGR
                     DAGRLTDEIIGGWQVAGIYMWESGTPLSWGNVIYTGNWHDFHNTPHNYQQSFNTSVFD
                     TRSKDANGNPVQPNQYNYRTFPLYALRSDPSNNFDCSVLKDFSVMERATLQLRVEAYN
                     ALNHPQFNSPNVSPTSQSFGTSTSQINSPRVLQLGARFVF"
     gene            complement(2272..4386)
                     /locus_tag="VFA02_05135"
     CDS             complement(2272..4386)
                     /locus_tag="VFA02_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU09266.1"
                     /translation="MGAVCAAALLGMTAGAQEFRATLTGRVMDPSGAVVPNAAVTITN
                     TGTGAATVVQSGASGDYYAPFLAPGAYNLSVVAPGFQKYVRVGVQLDTGAKVTVNIPL
                     VVGSASQTVTVAAQTPLLETASADAGQVLTTKEVEDLPSNGRSALGFARDEYAVVPKL
                     KHATSQVRPFDNSGASDFSLGGGNSQSNEIELNGTPNMEDSGRVSAFSPMLDAVDQVR
                     VDVFSSDASLGDTSGGTVDITTKGGTNQFHGSLLEFYETSALAAQPFFSTTSQSTHQN
                     QFGAAIGGPVWVPKVFNGRNKLFFFYAYEGFSTKSPGTTITSVPTEAERLGDFSALLG
                     VNKSYQLYNPYNATMSGGNVTRQPVPGNVFANAGLTLSPVAQAYLKYIPLPNYSGPTT
                     KSDGENNFIANTPTIYDYRSSQGRLDYNLSQADKLIFEVHRSNNEQTQTGVFPNIAEG
                     SSSITNIWGGMLDNVYVFTPKLFLDTRLGFSRTLQQSAIPSQGFDASALGYPSYMAAN
                     SKLPAMPRISFSDGASIPSLSTQPGSIEKFVTYQLFTNLTKDWGQHTLKTGVDFRLNK
                     LGTQNPGYSAGTFSFTNAAGNWVTAGTNKAAAPFGDSLAEFLLGLPMSGEYDINNSFL
                     YNNWYFAGFLQDHWKATHEISMDFGLRLEHETPITESHDRAVVGFDPNAVNSATAAAE
                     AAFAAESSKYPAVPGSFSRRAD"
     gene            4650..6245
                     /locus_tag="VFA02_05140"
     CDS             4650..6245
                     /locus_tag="VFA02_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZU09267.1"
                     /translation="MTWLRFLNPAIIGLYMAAIIALGLYFSRRQRTTDRYFVASRSIP
                     GWAMGISLLATIITSVTFIAYPGAAYAGDWSLIVPGIMIVAIPASIGVVVVPFFRHVV
                     GMSAFEYFALRFGAGVRLYGSAMFAAGHLCKMAFVLYLLALTVNSITGWNSTLLLVAT
                     AFVGILYALVGGFEAIIWADVIQGALLWVGVLLAIGFLLYLTPASPSAIFHAAVQAHK
                     FSFGPTAWNLRGPTILVLLIYGTFFYLQKYTADQTVVQRYLVARSDRQALRGILLGAA
                     LCLPVWTLFMLAGTLLWGYYHFSHEALPTFITRNDQVFPFFLSSHLPPGVGGIFLAAL
                     FGAGMAMLSSDLNCLSAVLVRDFYQRWKPGATDRQALSMGRIFVLINGAVALAGAGAL
                     AHSHGTVLALYYAVTSIVAGGLAGIFLLAFLSRRTTARAVYAAIFANLAFTIWATLTS
                     GKDPLISGSRFRFPWHEYMIGATGQMLVIAVGLAASRFLKPQSDTEEPMTLWKWLRAR
                     QRPGPVAVSSPTPLFKPHEWEKL"
     gene            6242..7066
                     /locus_tag="VFA02_05145"
     CDS             6242..7066
                     /locus_tag="VFA02_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZU09268.1"
                     /translation="MNRFHRALEHSKRPLLGASIYFYNPLFLEIVAELGFDVLWIEME
                     HAFITFAEAADLCRMAHGRNLLTMIRVPDARRESVLKAAECGPDMIDLPMVDSPEQVK
                     ELLSYAKFPPLGQRGFFSVSRSLNYGLVDNLSKAQQKLNDELSLLVQVETAEALDRLD
                     ELCSIDGVDIFIGPADLSASLGFPGETTHPKLLEAGRHITETAKKYGRRVATAVNHRE
                     AAAWVEMGVDLIFCANDIVCLRNGASTVMRETSELRNQRETVEGLVNHLEKPCAHG"
     gene            7128..7967
                     /locus_tag="VFA02_05150"
     CDS             7128..7967
                     /locus_tag="VFA02_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU09269.1"
                     /translation="MPVQEGKSKKAARRRPKHTSTDGLRESPDRYQSRAVTRALDVLE
                     RFPDEATAYSLKELSQQMRLPESSLFRLLVTLESRGYLRQDADGAYRLTDRVLRGKQF
                     ERASLIREKIHPLLLQMARSCNETTSLAYLFGHHIMVLDSIESFHDIRVINRVGRVLP
                     PYASSMGKAITAFQDRATMDRILDAYGVFRRTERTITDHRAIFEEFEKIRRQGHAFDR
                     GEATEGGVCIGVPILGPNKRVEAAVSVSVPDIRIDKAREQQLIQELQQATQKMASLLN
                     DLR"
     gene            7992..9185
                     /locus_tag="VFA02_05155"
     CDS             7992..9185
                     /locus_tag="VFA02_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZU09270.1"
                     /translation="MRFLKIFAIASVISIAGFAQTTTPGPATLRRNYARDIGHPLDGR
                     MRNEGHGLRAAYLSPIFESSHAANLLSLQNGDLLCFWFSGHWEGESGTAIVMSRLAAG
                     SPRWSTPVVVDRHEGESYQNPVGFQSPDGTIWLLHTTQPAGKGESQSRVLVLQSKDNG
                     KTWSSPAVLFDTPGSYVRNPVLISPGGNWMLPMYFTSEGSAGVAKIDRPIVAISSDQG
                     KNWKICDIPDAIARVQPSIFLSSGKGYVALLRSRRADFIYRSTSQDGCHWSAPVATSL
                     PNNNASIQTLRLPDGKILLAFNNTSAQMVDGKRRTGPRKPLSLALSSDDGETWHSIRD
                     LETGRPGVTDISKLPGREEYSYPALALGHDGRIYAAYTFRRETIKVVSFPEKWLTSGT
                     AAFKR"
     gene            9261..>10696
                     /locus_tag="VFA02_05160"
     CDS             9261..>10696
                     /locus_tag="VFA02_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HZU09271.1"
                     /translation="MHLRFSLFLAGTLLLSAALTVCAAENSPNGAFLLNPEDYWHYFS
                     EFSRQQLEATGHADASPWRWMQANIPWFDSSDKAFEEMYYFRWFSFQKHVVSTPRGFV
                     ITEFLSHVKWGGYYNTIVDAAPFHLYEARWLRDPHIAADDARFWMSADAAPRNYSIAI
                     ADAIRAVTLASGDTRLGAELLPAMKANYLAWEATHQDANGLFWSIDTRDAMEHSISGD
                     GYRPTLNSYMYADAAAISALAKQAGDSATASEFAAKAEDQRARIEKYLWNPSDQFYEV
                     LSPARDSGIRKEKKFVDPGTVLQFSGVRELIGYIPWDYNIPAPDHAVAWKQLFDPQGF
                     AGKFGPTTAERRSPRFRYKNSDKCQWNGPSWPFATTQVLIGMANLENNQQQNYVTRED
                     YYRLFQTYVRSQHLRLPSGKVIPWMDEDLDADTGEWIARDILAQRHSPEAGRGAYYNH
                     SGFADPLITGLIGLRPSEENTVVLHPLLP"
BASE COUNT         2265 a         3245 c         2795 g         2391 t
ORIGIN      
        1 ggaaggcagc atgggcgcgc tggttgcatc gccccagcag gcccgcttcg gacgcgccaa
       61 agctgtcgcg ctgccatccg attgccagac gtgcgatgtg cgctttgcct gcaacggcga
      121 gtgccccaag caccgctttg cacaaacatc tgcgggcgag tacgggctga attatctctg
      181 cgccggatac aagcacttct ttcgtcacat tgatccgtac atgcgcttca tggcaaatga
      241 actaaacaac catcgcgcac ccgcgcgggt gatggagtgg gcccgctcgg gccgcgctgc
      301 ggttgctcaa tctgcctttt ctgcttgtcg tcagtagcct gcctgaacgc agttccttgt
      361 tccgccttca gcatcaacgg cccggggcgc agtctgtccc atccatccac ccttttcaac
      421 aggagaccta tgaaagagaa acacattgtc agagtgttaa catccgcgca gtgtcacaag
      481 gcctcatccg gtgacactgc acagcgcaaa cggagtgtac aaaacctgtg aaaatgccgc
      541 tattgcagtc ttcgtttccc tgggatgcac gaaaaagtat gatcgtcgtt tcgggggcga
      601 ggagagtgtt ggcaggattg gctggatcct cacattgcta agattcttcc tctcttcagt
      661 cgtcagaatg acggagactg catagacgaa aaatgggcgt aagatgctgc aaacccaaat
      721 cttctcaaga tgtggagcac ccagccatcc cacgcaggcc cctaaaacgc aggccccttc
      781 acatttcggg gggcttgcat gggtacaacc aggtcaaaac tcaaagcggg caaagaactg
      841 ggcgagacga ggatgggtga gcgtgccgga ttctgagaaa atcagtttct catctggcca
      901 tgcgatccac tcagaagaca aagcgggccc cgagttgcag cacacgcggc gaattgatct
      961 ggctcgtaga tgtcccgaat gactgtgaag tcgggcttac attcggcgaa ttgaattgcg
     1021 gatggttcaa cgcgttgtat gcctcgacgc gaagctgcaa cgtggcgcgc tccatcacgc
     1081 tgaagtcctt cagcacagag caatcaaaat tgttcgatgg atcggagcgc agcgcataca
     1141 gaggaaacgt ccggtagttg tattggttcg gctgcaccgg attaccgttt gcgtccttgc
     1201 tgcgcgtatc aaacacagat gtgttgaagg actgctgata attgtggggc gtgttgtgaa
     1261 aatcgtgcca gttgcccgtg tagatgacgt ttccccaact cagcggcgtg cccgattccc
     1321 acatataaat gcccgccacc tgccatcctc caatgatttc gtccgtcagg cggcctgcat
     1381 ctctcccgaa ggccctgccc cggccaaagg gcagctcgta tgtggccgtc accgccaggt
     1441 gttgcggaaa gtcagaagcg ttggtcccat accagagtgg ccctcccgcg ttcagaggac
     1501 tggcggcagc cagcagccgc gaatactcat agttcacatt ggctgaaagc cccgatgaca
     1561 gacgcttcgt caaccgcgcc accagcatat tcaggtttga ggatgccgca gggaccagtt
     1621 gctcgctcac agaagagtat tccggaaatc tgctgaacag gctgctcagg gtcatcgtct
     1681 tgcctgtccc gatgctgctg ccctgaatca gcccgtaaaa cggattggtc actgcggacc
     1741 cgtatgtctt cgtcactgcc ggatcgtaat aggccgagcg gctcagcagg ttgagcggaa
     1801 aataattcag cggcgcagtg taagacatat ggacctggtg gttgccgata tagcccacct
     1861 gcaccaacgt attgtggccc aggtcgtgct gaatatcgaa actccagcgg atgctgtagg
     1921 ccgcccttgg ctttggattg gtaaacgtaa tcgcctgccc cagatacgta ttgattccct
     1981 ggctgctccc aaacggctgc cgcaacggat tcgacgcccc cggaaacgga tcagacaaag
     2041 tagcggcggg cgtcagcata ttgtcattgc tggacaccag tgtatttgca atgctgtacc
     2101 cataggcctg tggcgtgtag tagtcattga atgggttgta atacagcccc ataccacccc
     2161 gaaacaccgt ctttccatgt agaaaatccg gcgcataggc aagtcccatg cgcgggctta
     2221 cataaagcgc ttgcgttgag taattgctcc ggtggcccga tgaggcataa atcagtccgc
     2281 ccgtcgcgag aagctgccgg gcaccgctgg atactttgac gactccgcag caaatgccgc
     2341 ctccgcagcc gccgtcgcac tgtttacggc attcggatcg aagcccacaa cagcgcggtc
     2401 gtggctctca gtaattggag tctcatgctc cagtctcagg ccaaagtcca tcgaaatttc
     2461 atgggtcgcc ttccagtggt cctgcagaaa accggcaaaa taccagttgt tataaagaaa
     2521 ggaattgtta atgtcgtatt cgccgctcat cggcagcccc agcagaaact ccgccagtga
     2581 atcgccaaaa ggagcagcgg ctttgttcgt tcccgcagtc acccagttcc ctgctgcatt
     2641 cgtaaacgaa aatgttccag ccgaataccc cgggttctgc gtgccgagtt tgttcagacg
     2701 gaaatcaaca ccggtcttca gcgtatgttg tccccagtct ttcgtcaggt tcgtaaaaag
     2761 ctgatatgtg acaaacttct caatgcttcc cggctgcgtg ctcaggctgg gaatactcgc
     2821 tccatcactg aacgaaatac gtggcatggc cgggagcttg gaatttgcag ccatataaga
     2881 cgggtacccc agcgcggagg catcgaatcc ctggctggga atcgcgctct gctgcagcgt
     2941 gcgtgaaaag ccgagtcgcg tatccagaaa cagtttcggc gtaaagacgt acacgttgtc
     3001 cagcatcccg ccccagatgt tggtaatgct ggaggagcct tccgcaatgt taggaaagac
     3061 gccggtctgc gtctgctcat tattgctgcg atgtacttca aagatgagct tgtcggcctg
     3121 actcaggttg tagtccagac ggccctggct ggaacggtag tcataaatgg tcggcgtgtt
     3181 cgcgatgaag ttgttctcgc catctgattt ggtcgttgga ccgctgtagt tcggcagagg
     3241 aatgtatttc agataggcct gtgctacagg actaagcgta agaccggcat ttgcaaaaac
     3301 gtttccagga accggctgtc gcgtcacatt gccgcccgac attgtcgcgt tgtacgggtt
     3361 gtagagctga tagcttttgt tcacccccag cagagcagaa aagtctccca accgctccgc
     3421 ttccgttggc accgacgtaa tcgtcgttcc cggcgacttc gtgctgaacc cttcataggc
     3481 atagaagaaa aacagcttgt tgcgtccgtt gaacactttg gggacccaaa ccggtccgcc
     3541 aattgccgcg ccaaactggt tctggtgcgt ggactgcgaa gtagtactga aaaaaggctg
     3601 tgcagccaat gctgatgtct cataaaactc cagcagagag ccgtggaact ggttcgtacc
     3661 gcctttcgtc gtaatgtcta cagtgccgcc tgacgtgtct cccagcgacg catctgaaga
     3721 gaaaacatcc acccggacct ggtccactgc atccagcatc gggctaaagg ctgaaacgcg
     3781 tccgctgtct tccatattcg gcgtgccgtt cagctcaatt tcgttgctct gtgagtttcc
     3841 gccgcccaga gaaaaatcgc tcgcccccga attgtcaaaa ggccgcacct gcgaagtggc
     3901 atgtttcagc ttgggcacca ctgcatattc atcgcgcgca aaacccagcg cagagcgtcc
     3961 attgctgggc aggtcctcta cttcttttgt tgtgagcact tgtccggcat cggccgatgc
     4021 cgtttccagc aaaggagtct gcgcggccac ggtcacagtt tgcgaagcgc tgcccacaac
     4081 caaaggaata tttaccgtca ccttcgcgcc tgtatcgagc tggacgccca ctctcacata
     4141 tttctgaaac ccgggcgcaa caaccgacag attgtatgca ccgggagcga ggaagggcgc
     4201 ataatagtcg cccgacgcgc cggactgcac cacggtagca gcccccgtgc ccgtattcgt
     4261 aatggtgact gccgcattcg gcacaactgc tcctgaagga tccatcaccc gcccggtcaa
     4321 tgtcgcccgg aactcctgcg cacctgcggt catcccaagc agggccgcgg cgcagaccgc
     4381 ccccaaaaac atcctgaaac gcatggcact ccctcgaaat caaggcgcaa ttgaaaatca
     4441 tctgttttga aattgtcaag atattatgaa aataattttc aaattattcg gaaaatttaa
     4501 aaaaatttgc atatttgatg gtacagttgc atattcagaa gtactggcag cctgggcgca
     4561 aacatgcatt tttccttacg cgcaaaatct tccggcaggg ccctttgtag atcaggtcat
     4621 gtaccatgcc cgcaggagaa gcccagccga tgacctggct tcgtttcctc aacccggcca
     4681 tcatcggcct gtacatggcc gccatcattg ctctgggcct gtatttctcg cgccgccagc
     4741 gcactacaga ccgttacttc gtcgccagcc gcagcattcc cggatgggcg atgggcattt
     4801 ctctgctcgc cactatcatc accagcgtca cgtttattgc ctatcccggt gcggcctatg
     4861 cgggcgactg gtctctgatc gttccgggca tcatgattgt ggccattccc gccagcatcg
     4921 gcgtagtggt cgttcccttc ttccggcacg tcgttggcat gtcggctttt gaatattttg
     4981 ccctgcgctt tggcgcagga gtccggctct atggctcggc catgtttgcc gccggtcatc
     5041 tctgcaaaat ggcatttgtt ctttatctgc tggcgttgac ggtcaacagc atcactggct
     5101 ggaacagcac tctgctgttg gtcgctacgg cctttgttgg catactgtac gctctcgtcg
     5161 gcggttttga ggccatcatc tgggccgacg tcattcaggg cgcactgctc tgggtcggcg
     5221 tccttctggc gattgggttc ctgctctatc tcacgccggc ctcgccatcg gccatatttc
     5281 atgcagccgt acaggcgcac aaattcagtt tcggtccaac tgcatggaat ttgcgcgggc
     5341 ctaccatcct tgtccttttg atatacggga cgtttttcta cctgcaaaag tacaccgcag
     5401 accagaccgt tgtgcagcgg tatctcgtag cgcgcagcga ccgtcaggca ctccgcggaa
     5461 ttctgctcgg ggccgcgctc tgcctgcctg tatggacact cttcatgctg gccggaacgc
     5521 tactatgggg ctattaccac ttcagccatg aggcgcttcc cacttttatt acgcgcaatg
     5581 accaggtatt ccctttcttt ctctcctcgc accttccgcc cggagtgggt ggcatttttc
     5641 ttgctgcgct cttcggtgca ggcatggcca tgctctcctc tgacctgaac tgcctttccg
     5701 ccgtactggt gcgcgacttc taccagcgct ggaagcccgg agctactgac cgccaggccc
     5761 tctcgatggg ccgcatcttc gtgcttatca atggagcggt tgcgctcgct ggagcaggtg
     5821 cgcttgccca cagccatggc acagtactgg ccctctacta tgcagtcact tcgattgttg
     5881 ccggcgggct tgccggcatc tttctgctgg cattcctcag cagacggaca acagcgcgcg
     5941 cggtctatgc agcgattttt gccaacctgg ccttcaccat ctgggccacg ctcacctcgg
     6001 ggaaagaccc tctgatttct ggcagccgct tccgttttcc ctggcacgag tacatgatcg
     6061 gcgccaccgg tcagatgctt gttatcgctg ttggacttgc cgccagccgg ttcctgaaac
     6121 ctcaaagcga tacagaagag cctatgaccc tctggaaatg gctccgggcg cgtcaaagac
     6181 ccgggccagt tgcagtctct tcacctacgc ctcttttcaa gccacatgaa tgggagaaac
     6241 tatgaaccgg tttcaccgcg ctcttgagca cagcaagcgt ccgctcctcg gagcatccat
     6301 ctacttctat aatccgctct ttctcgaaat cgttgccgag ttgggttttg atgtgctctg
     6361 gatcgagatg gaacacgcct ttatcacctt tgccgaggcc gccgacctct gccgcatggc
     6421 gcacggccgc aatcttctca ccatgattcg cgtgcccgac gcccgtcgcg aaagcgtgct
     6481 gaaggctgcc gaatgcgggc ctgacatgat tgacctgccc atggtggatt cgcccgaaca
     6541 ggtgaaagag ctgctgagct acgcaaagtt tccacccctc gggcaacgcg gcttcttcag
     6601 cgtctcccgt tcgctgaatt acggcctcgt agacaatctc agcaaggccc agcagaaact
     6661 caacgacgag ctttccctgc ttgttcaggt cgaaaccgcc gaggccctcg accggctcga
     6721 tgagctttgc tcgatagacg gcgtggacat cttcatcggt ccggcagacc tctccgccag
     6781 cctcggcttt cccggcgaaa ccacgcatcc aaagctgctc gaagcaggcc gtcacatcac
     6841 cgaaacagca aagaaatacg gccgtcgcgt ggcaaccgcg gtcaatcatc gtgaagccgc
     6901 cgcatgggtg gagatgggcg tggacctcat cttctgcgcc aatgacattg tgtgcctccg
     6961 caacggcgcg tctacggtca tgcgcgagac ttcagagctg cggaaccagc gggaaaccgt
     7021 cgaaggtctg gtgaaccatc ttgaaaagcc ctgtgctcac ggctaatatc ggagcagttt
     7081 attatttttt tgaaagtcac tttcaatttt ttgaggacat cttcgccatg ccagtacagg
     7141 aaggaaaaag taaaaaagcg gcccgccgac gtcctaaaca caccagtaca gatggcctgc
     7201 gggaatcacc agaccgctac cagtcgcgcg ccgtcacgcg cgcgctcgat gtgcttgagc
     7261 gttttcctga tgaagccact gcttactccc tgaaagagct ttcccagcag atgcggctgc
     7321 cggagtcctc tctttttcgt ctgctggtta cgcttgaatc ccgcggatat ctccgtcagg
     7381 acgccgatgg cgcataccgc ctcaccgacc gcgttctgcg cggaaagcag ttcgagcgtg
     7441 catcgctgat acgcgaaaaa atccatccac ttctgctcca aatggcgcgg tcttgcaatg
     7501 agaccaccag tctcgcttat ctcttcggcc atcacatcat ggtgctcgac agtatcgaga
     7561 gttttcatga tattcgcgtc atcaaccgcg tcggccgcgt gctgcctcct tacgccagtt
     7621 ccatgggcaa ggccattacg gcctttcagg accgcgccac catggaccgc atcctcgatg
     7681 catatggagt tttccgccgt acagagcgca ccattaccga ccaccgcgcc atcttcgaag
     7741 aattcgagaa gatccgccgc cagggccatg ctttcgaccg cggcgaagcc accgaaggtg
     7801 gcgtctgcat cggcgttccg atcctcgggc ccaacaagcg ggtcgaggcc gccgtcagtg
     7861 tttccgtacc cgatatccgc atagacaagg cccgcgagca gcagctgatt caggaactcc
     7921 agcaggccac acaaaagatg gcttcgttgc tcaatgatct tcgctgaacg atctggacaa
     7981 aggaatttgc tttgcgattt ctgaaaattt ttgccatcgc ctctgttatt tctatcgccg
     8041 gttttgccca gacaaccacg cctggtcccg ctacactgcg ccggaattat gccagagaca
     8101 ttggccaccc actcgacggc agaatgcgaa atgagggcca tgggctgcga gcggcgtatc
     8161 tttctcccat ctttgaatca agccacgcag ccaatctgct gtcattgcag aacggagacc
     8221 tgctctgctt ctggttcagc ggacactggg aaggtgaatc cggcaccgcc atcgtgatgt
     8281 cgcgcctggc tgccggaagc ccgcgctgga gcactcctgt cgtcgttgac cggcatgaag
     8341 gcgagtccta tcaaaacccg gtaggatttc agtcgcccga tgggaccatc tggttgctcc
     8401 atacaacaca gcctgccggc aaaggtgaga gccagtctcg cgtgcttgtg cttcaatcaa
     8461 aagacaacgg aaagacctgg agctcgccgg cggtcctctt cgacacgcca ggttcgtatg
     8521 ttcgcaatcc ggtcctgatc agccccggcg gcaattggat gctgcctatg tacttcacca
     8581 gcgaaggcag cgccggagtc gccaaaatag accgacccat tgtcgccatc tcttcagacc
     8641 agggaaaaaa ctggaagatt tgcgacatcc ctgacgccat cgcacgggtg cagcccagca
     8701 tctttttgag ttccggcaaa ggatacgtgg cactcctgcg cagccgtcgt gccgatttca
     8761 tctatcgcag cacgtcgcag gacggctgtc actggtctgc tcccgtggcc accagcctgc
     8821 ccaacaataa cgcttcgatt caaaccctgc ggctgcccga tggcaaaatc ctgctggcct
     8881 tcaacaacac ttctgcgcaa atggtggacg gcaaacggcg cactggcccg cgtaaaccac
     8941 tttccctcgc cctgtcttcc gatgacggcg aaacctggca ttccatccgc gatctcgaaa
     9001 ccggccgccc cggcgtaaca gacatcagca aactccccgg acgcgaagag tattcttatc
     9061 cggcccttgc tctgggccac gatggacgca tctacgccgc ctacaccttt cgccgtgaaa
     9121 ccatcaaggt cgtctctttc ccggagaagt ggctgacatc aggcactgca gctttcaaac
     9181 gataaagtta ttttcatttt catgcatagt ttttatttta ttgaagattc atccactttc
     9241 cagttacagt tattttgcca atgcatctgc gcttttccct gtttctggca ggcacgctcc
     9301 tgctttccgc tgcactgaca gtctgtgctg cggaaaactc ccctaacggc gcgtttctgc
     9361 tgaatcctga agattactgg cactacttca gcgaattttc ccgccaacaa ctcgaggcca
     9421 ccggccacgc agacgccagt ccctggcgct ggatgcaggc caacatcccc tggttcgact
     9481 cctctgacaa ggccttcgag gagatgtact acttccgttg gttttccttc cagaagcatg
     9541 tcgtttctac gccacgcggc tttgtcatta ccgagttcct gtcgcatgtg aaatggggcg
     9601 gatactacaa caccatcgtg gatgccgccc cgttccatct atacgaagca cgctggctgc
     9661 gcgaccccca tatcgctgcc gatgacgccc gcttctggat gtctgcggat gctgccccgc
     9721 gcaactatag catcgccatc gccgacgcca tccgtgccgt cacgcttgcc tcaggcgaca
     9781 cgcgcctcgg cgcagagctg cttcccgcaa tgaaagccaa ctacctcgcc tgggaagcga
     9841 cccaccagga cgccaacggc ctcttctggt ccattgatac ccgcgatgcg atggagcaca
     9901 gcatcagcgg cgatggatac cgtcctaccc tgaacagcta tatgtatgcc gatgctgcgg
     9961 cgatttctgc gttggcaaag caggccggcg attccgcaac ggcaagcgaa tttgccgcca
    10021 aagccgagga ccaacgcgcg cgcatagaga aatatctctg gaacccgagc gaccagtttt
    10081 acgaagtact ctcacctgca cgcgattccg gcattcgcaa agagaaaaaa ttcgtagacc
    10141 ccggcaccgt gctccagttc tctggcgtcc gcgaactcat cggctatatc ccctgggact
    10201 acaacatccc tgccccagac cacgccgttg catggaagca gctcttcgat ccgcagggct
    10261 ttgccggaaa gtttggcccc acgacggctg agcgccgcag cccacgcttc cgctacaaaa
    10321 acagcgacaa atgccagtgg aacggcccct cctggccttt tgctacaacg caagtgctta
    10381 tcggaatggc caaccttgag aacaatcagc agcagaatta cgtcactcgc gaggactact
    10441 atcgtctgtt tcagacctat gtgcgttcgc agcacctccg cctgcccagt ggcaaagtga
    10501 ttccgtggat ggatgaagat ctcgacgccg atactggcga atggatcgcg cgagacatcc
    10561 tcgcccagcg ccacagcccg gaggccggac gcggtgccta ctacaaccac tccggctttg
    10621 ccgacccgct gattaccggg ctgattggcc tgcgcccgtc agaagaaaat acggttgtgc
    10681 ttcatccgct gctgcc
//
LOCUS       DASRBV010000134         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_565199, whole genome shotgun sequence.
ACCESSION   DASRBV010000134 DASRBV010000000
VERSION     DASRBV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565199"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFA02_05165"
     CDS             <1..459
                     /locus_tag="VFA02_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09272.1"
                     /translation="WQPQWGQYQKRDKAGHHFVDGGVLSNFPLRLIAESTPDVVAMMG
                     PTDPNGALNLGLFLDASVMVPGAELSDTVRPRLRVADRVTHLMDTLTDSSDLAVMRQY
                     QSDVCRIPVGGYGTTEFRMSSARQQLLIASGKAAMEQYLKCKVPVTADAS"
     gene            466..639
                     /locus_tag="VFA02_05170"
     CDS             466..639
                     /locus_tag="VFA02_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09273.1"
                     /translation="MRGRSSYIIWFVGAVAWFFDAALQVYSGTRVHAFVAIVICLLFL
                     VAGVYFRRQVLRK"
     gene            complement(691..2085)
                     /locus_tag="VFA02_05175"
     CDS             complement(691..2085)
                     /locus_tag="VFA02_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HZU09274.1"
                     /translation="MQTTEILKKFGPKLNGPLPGPNAKRIVAEDDRLISPSYTRSYPM
                     VAKSGKGVRVTDVDGNEFLDFAAGIAVTSTGHCHPEVVAAIQKQAAELIHMSGTDFYY
                     ESLVTFARRLSAIAPMPGPHKFYYGNSGAEAIECALKIARYHTQRQHVIAFFGAFHGR
                     TMGALSLTASKPQQRRRFAPLVPGVTHVPYPNVYRGCTGGSEEQEKFALDCARYIEEK
                     LFKTTLPPEEVAAIFVEPIQGEGGYVVAPAIFMQELRRICDKYGILLVADEVQSGAGR
                     TGKWWAIEHSGVQPDIVCSAKGIASGMPLGICMTRAEIMDWKPGSHASTFGGNPVSIA
                     AALATMDVLEREGIANAANVGEQMLGRLRQWPSKHAIVGDVRGLGLMIGVEVVKNQAT
                     REPAGAWRDRIVELAFERGLLVLGCGETSIRLAPPLIVNEHEAAIALDILEECISAVE
                     KEHTEAGVLAASAV"
     gene            complement(2229..2369)
                     /locus_tag="VFA02_05180"
     CDS             complement(2229..2369)
                     /locus_tag="VFA02_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09275.1"
                     /translation="MCSKGRISGTIPSPHSWNRAFARAIAPRKHLRVLPTRTRPSLFG
                     SD"
     gene            complement(2431..>3693)
                     /locus_tag="VFA02_05185"
     CDS             complement(2431..>3693)
                     /locus_tag="VFA02_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09276.1"
                     /translation="DYIASMKQFNMRRWNDGDDFLVNYVGHPMQGAVGAFIEIQNSPW
                     QSRVRWNQPGYWNSRWKAFWWTTLYSTHSEISPLGEAGLGNEGGYTYDVKCILHCGPK
                     DPPSGKYTNNTGWVDFIVTPTVGMLWVFAEDWIDKDISDRIADHAGPDAVYPKVIRGA
                     LNPARTFANLLRGHKPWYRDYDHPIMEFGSGIHFTRLENATGDYGEQRRRWPRFQVAP
                     FFRSYSTTVTTPNCTYCRRTVTGGGAQVSVHLWRWLYADTEVARYPGASPAPSDRAGG
                     DLITGFFGFRGGVNTPNYALNLSLRPGFLRFNDAYLTSPEEGGPAPERGTITHFAWNI
                     MLAGDYKLTRNLAIRAGIEETLVRYRQSCLDASGTGTPYPLRWLVQGTECSQHPGIGQ
                     PPYLSWLSREDFTNRGSWAYQIGPVFSF"
BASE COUNT          736 a         1067 c         1104 g          786 t
ORIGIN      
        1 tggcagcccc agtggggcca atatcaaaag cgggacaaag ccgggcacca ttttgtagat
       61 ggtggagtgc tgtcgaattt tcctctgcgg ctgatcgccg aatccacgcc agacgtggtg
      121 gccatgatgg gccccactga cccgaatggc gccctgaatc tggggttgtt tctcgatgcc
      181 tcggtgatgg tcccgggcgc ggaactttca gacaccgtgc ggccgaggct gcgtgtggcc
      241 gaccgtgtga ctcacctgat ggacaccctg accgacagct ccgaccttgc ggtcatgcgc
      301 caataccagt ccgatgtctg ccggattccc gttggcggct acggcacaac agagttccgc
      361 atgtcttccg cgcggcagca attgctgatt gcttccggaa aggccgcgat ggagcagtat
      421 ctgaaatgca aagtacctgt aactgctgat gcatcttaat gagaaatgcg aggacggtct
      481 tcctacatta tctggtttgt tggagcagtt gcctggtttt tcgacgcggc actgcaggtc
      541 tattccggga cccgcgtcca cgcctttgtg gccattgtga tttgcctgct ttttcttgtc
      601 gcaggggtgt atttccgcag acaggtcctg agaaaataga aggaccgtga gcggatgccc
      661 caggtctcgc ggagacctgg gttcgaatcc ttacacagcc gacgcagcca gaactcccgc
      721 ctccgtgtgc tctttctcca cggcggagat gcactcttcc agaatgtcga gcgcaatcgc
      781 tgcctcatgt tcgttcacaa tcagcggcgg agcaagccgg atcgaagtct cgccacagcc
      841 cagcaccagc aggccgcgct cgaaggccag ctcaacgatg cggtcgcgcc atgctccggc
      901 aggttcgcgc gtggcctggt ttttcaccac ctcgacaccg atcatcaggc cgagtccgcg
      961 cacatcgccc acgatggcgt gctttgacgg ccactgccgc aggcggccaa gcatctgctc
     1021 gccgacgttc gcggcattgg caatgccttc gcgttccagg acatccatcg tcgccagcgc
     1081 ggcggcaatc gagacggggt ttccgccaaa ggtagaggcg tgcgatccgg gtttccagtc
     1141 cataatttcg gcgcgcgtca tgcagatgcc cagcggcatt cccgaagcaa ttcctttggc
     1201 gctgcaaact atgtcgggtt gcacgccgga gtgctcaatc gcccaccatt tgcctgtgcg
     1261 cccagcgccg gactgcactt catccgcgac aagaagaatg ccgtatttgt cgcagatacg
     1321 ccgcagttcc tgcataaaaa tcgcaggtgc aaccacgtag ccgccttcgc cctggattgg
     1381 ctcgacgaag atggcggcca cttcctcagg cggcagtgtg gtcttgaaga gcttttcttc
     1441 gatgtagcgg gcgcagtcca gtgcgaattt ttcctgctct tcggagcctc ccgtacagcc
     1501 gcggtagaca ttcggatacg gaacgtgtgt cacaccgggc accagcgggg cgaagcggcg
     1561 gcgctgctgc ggctttgatg ccgtgagcga aagcgcaccc atggtgcgtc cgtgaaatgc
     1621 tccgaagaag gcaatcacat gctggcgctg cgtgtggtag cgggcaatct tcaatgcgca
     1681 ctcaatcgct tctgcgccgg agttgccgta atagaacttg tgcggcccgg gcatgggagc
     1741 aatcgcagac agccgcctgg caaatgtgac aagcgattca tagtagaagt ccgtgcccga
     1801 catatggatc agctcggcgg cctgcttctg gatggcggca accacttcag gatggcagtg
     1861 tccggtagaa gtcacggcga ttccggcggc aaagtccaga aactcatttc cgtccacgtc
     1921 agttacgcgg acacccttgc cgctctttgc caccatggga tacgaacgcg tatagctggg
     1981 cgaaatcagg cggtcatctt cggcgacgat gcgtttcgca ttcgggccgg gaagcgggcc
     2041 atttaacttt ggcccgaatt tcttcagaat ttcagtcgtc tgcatgtgct ttctccttaa
     2101 tatgcctggg ttttggttga tggatggcgc atggggagaa gtctctcttc cggcgttgat
     2161 actctggcgg ggtacactgc gctggtttgg ggcggtgttt ggccgtcgag cacaacgggc
     2221 agcggtgact agtcgctgcc gaagaggcta ggtcgcgtgc gggtaggcag gacgcgaaga
     2281 tgcttcctgg gagcaatggc gcgcgcaaag gcccggttcc agctatgcgg gctggggatg
     2341 gtcccggaaa tgcgtccttt gctgcacatt gcgttgcact gagaatagca cgccagagaa
     2401 gccgctgtga agataaggca acactgaagc tcaaaagctg aacaccgggc ctatctgata
     2461 ggcccagctt ccccggttcg tgaagtcttc gcgcgaaagc catgacagat acggtggttg
     2521 tccgattccc ggatgctgcg agcactctgt cccttgtacc agccagcgca gcggataagg
     2581 cgtgccggtt ccggaagcat ccaggcagga ctggcggtat cgtacaagcg tctcctcaat
     2641 tccggcacga atggccagat tgcgcgtgag cttgtaatct cctgccagca tgatgttcca
     2701 ggcgaagtgc gtaatggtcc cgcgttcagg cgcaggccct ccttcttctg gtgaggtgag
     2761 atacgcatca ttaaatcgca ggaacccagg gcgcagagaa agattcagcg cgtaattcgg
     2821 cgtattcacg ccgccgcgaa aaccgaagaa cccggtgatg aggtcgcctc ccgcacggtc
     2881 ggatggcgca ggacttgctc ccggatagcg cgccacttcc gtatcggcat aaagccagcg
     2941 ccacagatgg acagagacct gcgcgccccc gcctgtgaca gtgcgccggc agtaggtgca
     3001 gttcggtgtg gttacagtgg ttgaataaga ccgaaagaag ggcgccacct gaaagcgcgg
     3061 ccagcggcgc cgctgctcac catagtcgcc tgtggcgttt tccagcctgg tgaaatgaat
     3121 gccggagcca aactccatga tggggtggtc gtagtcgcgg taccagggtt tgtgtccccg
     3181 gagaagattg gcgaaagtac gcgcaggatt cagagcgccg cgaatcacct tcggatatac
     3241 agcatcaggc ccggcatggt cagcgatgcg gtcgcttatg tctttgtcaa tccaatcttc
     3301 ggcaaagacc cagagcatcc caaccgttgg cgtcacaata aagtccaccc atccggtgtt
     3361 gttcgtgtat ttgccacttg gcgggtcctt cggtccacaa tgcagaatgc actttacgtc
     3421 ataggtgtag ccgccttcgt tgcccagtcc ggcttcgccc agcggagaga tctcagagtg
     3481 tgtgctgtag agcgtcgtcc accagaaggc cttccagcgc gagttccaat acccgggttg
     3541 gttccagcgg acacgcgatt gccacggcga gttctgtatt tcgataaatg cgcctactgc
     3601 tccctgcatg ggatgaccga cataattcac cagaaaatcg tccccatcat tccaccggcg
     3661 catgttgaac tgcttcatgg aagcaatgta gtc
//
LOCUS       DASRBV010000135         7006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_615684, whole genome shotgun sequence.
ACCESSION   DASRBV010000135 DASRBV010000000
VERSION     DASRBV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615684"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFA02_05190"
     CDS             <1..502
                     /locus_tag="VFA02_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09277.1"
                     /translation="YTWSHYGNNTSGIYWFPENQYAPDAEWGPADWDQRHHFGFYGMF
                     NPEHLLNLGVGVFANTGKPWTITTGTDAYGTNLFNARPDDVPRNSETGPDYADLDLRW
                     GYDFKLHPKEMDKSPTLGLSASAFNVLNHPNGSYVDTVEGSPDFGDVTSAYPPRRMQM
                     AMRFVF"
     gene            complement(1711..2577)
                     /locus_tag="VFA02_05195"
     CDS             complement(1711..2577)
                     /locus_tag="VFA02_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU09278.1"
                     /translation="MELHQLRYICAIAETGSFSRAAERCHVAQPSLSQQVSKLEDELG
                     ARLFDRLGRRVRLTDTGRAFLPRARAILQEVEAARFEVDDVRNDSKGSITVGVIPTIA
                     PYFMPPRVATFARKFPEATLRIVEETTPILVEGVRDLSLDMAILSLPLRHREFEITPL
                     LKEPLYAVLPCEHALAKRREISLKDLRAEPFVLLRDSHCFHDVAVAACQRARVSPHVV
                     FESGQFTSLLGMVSAGVGVSIVPAMAVDEAPDRAFVRIGDQKAIRTIVAVVLRGRSLN
                     RIQKSFLEHLRG"
     gene            complement(2645..3442)
                     /locus_tag="VFA02_05200"
     CDS             complement(2645..3442)
                     /locus_tag="VFA02_05200"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZU09279.1"
                     /translation="MYPFIHIGRFSIGTFGIMLWLAAVCACWVLHRNFRRWKIDADAI
                     GIVAISTVAGVIGAKLWHVLESPQLLRQDPFGMLLDRAGFAWFGGLVLGILSLLWQSR
                     GAGLSKLGMLDLSAPAAAVGYGVGRLGCLTSGDGDYGIPTNLPWGMSFPNGLVPTPPG
                     VRVHPTPIYELIVALLIAWFLWKRGRPEAAHPAGQITGEYLILSGVARFLVEFVRINP
                     KIYWGMSNAQMASLGSVLAGILLIVWARRTGRSVLASPAVSAVKANP"
     gene            3477..4022
                     /gene="hpt"
                     /locus_tag="VFA02_05205"
     CDS             3477..4022
                     /gene="hpt"
                     /locus_tag="VFA02_05205"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZU09280.1"
                     /translation="MSTQTTPALEVLFSREQIAQRVAELGRQIDQDYAGQNIVLLGVL
                     KGAAIFLADLARNITVNNTFDFVAVSSYGKGQKTSGAVRLIKDVDQPIEGKNVIVVED
                     ILDTGLTLDFLRKLFQQHNPRSLRIAALLDKPSRRLVKIEADYVGFSIPNKFVVGYGM
                     DYAERFRNLPDICLMPPDFPE"
     gene            4046..4795
                     /gene="deoC"
                     /locus_tag="VFA02_05210"
     CDS             4046..4795
                     /gene="deoC"
                     /locus_tag="VFA02_05210"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZU09281.1"
                     /translation="MTTKVVSIEEEFSNGFFDIAAFTHHAFSSRSNLASIFDHTLLKP
                     DATRDQVVRLCEEAAQYGFACAMVNPAWVSTACSVLSGTGIRVGTVLGFPLGASLSIT
                     KREEAVELIKLGAHDLDMVLNIGLLKSGMNDRVQQDIHGVVEVAHEAGAIVKVILETC
                     LLNVEEKLRASEVAIAAGADFLKTSTGFSTGGATPEDVALLRGVAGGRSGVKASGGIR
                     TLAETRAMLEAGANRIGASASVSILREFDAL"
     gene            4899..6737
                     /locus_tag="VFA02_05215"
     CDS             4899..6737
                     /locus_tag="VFA02_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZU09282.1"
                     /translation="MSKEQVRARTGDEARPEDHAFEGDYRPPSPIPAKTQVRVEPLQT
                     EFLVRLADALNTTLDLQTLMQRTADMVRAVIDYRIFAILLLNDRTNELRMRFQIGHTP
                     EAERMRIRMGKGIVGQAAQTREPVLVPDVTKTENYINVNPDVRSELAVPLIVKNRVIG
                     VIDIQSEQPDYFQQEHLHLLKLTASRIGQAIENARLYTRVARQAQMLQVLNEISREIT
                     SILDLESLLERIGTLLRRIIDYQMFSIWIVNERDHVLENRFALRFGERFYPDEKLPLD
                     QGLVGAAIVERRLIHAPDVRKDPRYRMFNPETRSEMAMPLIYKGKVIGILDIEHTRPH
                     FFNEDHERAITTLAAQVAISIENARLYQRVAQQEQRLEDDLAMAREVQLRLLPSVKPK
                     HARAEFSARFLPARTLGGDLYDFISYDGNRSAIVLGDVSGKAAPAALYAALVSGIMRS
                     EATQKLSPSEMLKTINDALQERHVDSQYVTMLYAVWNDENLTLQLSNAGAVQPLFCRD
                     GEVETVKAEGFPLGLFPNVKYEEFTLSTQPGDSLIFYSDGIVDAQNAVGDMFGSERLA
                     AVVQKNMHKSASKIADAIMSEVGKFQKGVERFDDETVVVLKVLGGK"
     gene            6876..>7006
                     /gene="rpsT"
                     /locus_tag="VFA02_05220"
     CDS             6876..>7006
                     /gene="rpsT"
                     /locus_tag="VFA02_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZU09283.1"
                     /translation="MANHVSALKRARQTERKTAVNRANKSRVRGAIRSLREALAKGDV
                     "
BASE COUNT         1622 a         1897 c         1857 g         1630 t
ORIGIN      
        1 gtatacctgg agccactacg ggaacaacac cagcggcatt tactggtttc cggaaaacca
       61 atatgctcct gatgcggaat gggggcctgc cgactgggac cagcgccatc attttgggtt
      121 ctatggcatg tttaacccgg agcaccttct gaatctgggg gtaggcgtgt ttgcgaatac
      181 aggtaaaccg tggaccatca ccaccggtac agatgcctac gggacgaacc tgtttaacgc
      241 gcgccccgat gacgtaccgc gcaattcgga aaccggcccc gactatgctg atcttgatct
      301 gcgctggggc tatgatttca agctgcatcc caaagagatg gacaaaagtc cgacgcttgg
      361 tctttctgca tctgccttca acgtgttgaa tcatccgaat ggctcctatg tggacacagt
      421 ggaagggagt cctgatttcg gagatgtgac cagtgcgtat cctccacgga gaatgcagat
      481 ggcgatgcgg tttgtgtttt agagtatttt cgctgattgt cattctgttg gcggggaatc
      541 ttagcaggat tggctaaatc cttatcgcta agattcttcc tccctgcggt cttcagaatg
      601 acggacttta cataaaacga taaattcgtc catttactga agttagctgg aatttgttct
      661 cgcacttgct tcgccttgcg ccctgaacga ccctaccccc ctcttttgta cggggatcgt
      721 ttattttcaa caacttacat ccgtttctct gtacggagat cctttatttt caatgagtta
      781 cactgcattg gtgcaaatca ttgattttaa aggacaagtg taaggggtta cctgcgcttt
      841 cagtcgctga gattcttctt ccctgcggtc gtagaggccc tcggtagtgg atacgggtca
      901 ggtaccccct cccctggctt gtactggaat cctttgtttt cagtaactta cgagcaatgg
      961 cttgtacggg aatcgtttgt tttcaatgag ttagctagaa gcggtcagca gtcagcgcct
     1021 gcttcgcagg ctgttagcgg tcagcgcccg gttgccagtt gccagttccc cagttcactg
     1081 gctttccata gataaaccag gtatatagaa aagtcatgcg attattttga tgtaagttgt
     1141 tgaaagtatt agatattcgt tgctttttat accttcgttt ttcgtgaaat ttgataccta
     1201 gattgtctac tgatagccag gcaaatgggt ggcaggtttt tctgctgtat gttattgaaa
     1261 atgagctggt tttgcaatgt ttttgtatct catttctgca tcccgcagct tccctagact
     1321 gtctacttat ctggggcatc cgggacgcgg cgtaattttc gactaaattc ttcaaaaaac
     1381 tcgcgtaatt tcttttttat cgcccatttc tgcgttgttt atccatagat tgtctatgga
     1441 tgccggtgtc cagtgctttt catttgttcg cgcttcgaga cgtgggcacc cggcttctgc
     1501 cacgaccatt tatttttgcc ctttctttca ggatatcaag cggaagggag gaatccggac
     1561 aaaggttggt ggggcttatc ttattgaaag agaataaatt cgatggattt attactttcg
     1621 taagggttga cagccggatt tacggatttc gtttcactgg gattccacga aaaggacgcc
     1681 cggcatgtac aaatgtcagc tctggattcg ttaaccccgc aggtgttcca aaaatgactt
     1741 ttgtatccgg ttgagggaac gtccacgcaa aactacagcc actatcgtcc gaattgcctt
     1801 ctgatcgccg attcggacaa aggcccggtc gggcgcctca tccacagcca ttgcagggac
     1861 aatggaaaca cccacgcctg cggagaccat tcccagcaaa ctggtgaact gtccactttc
     1921 aaagacaaca tgcggactta cacgagcgcg ctggcaggcc gcaacggcaa catcgtgaaa
     1981 gcaatggcta tctcgcaaca gtacgaatgg ttcggcccgc aagtctttca aggaaatttc
     2041 gcgacgtttt gccaaagcat gttcgcatgg cagaacggcg tagagcggct ctttcagcaa
     2101 cggtgtaatc tcaaactccc ggtgccgcaa gggcagagac aagatcgcca tgtcgagtga
     2161 taaatcgcgc acgccttcta ccaggattgg cgtcgtctct tccacaattc gcagcgtagc
     2221 ttcaggaaat ttacgcgcga atgtggcaac acgtggaggc atgaagtaag gtgcgatagt
     2281 tgggatgact ccgacggtga tactgccttt actgtcattg cgcacatcgt ccacttcaaa
     2341 gcgcgcggcc tctacttcct gcagaatggc ccgggcgcgt gggagaaacg cccggccagt
     2401 atcagtaagg cgcacacggc ggccaagcct gtcaaacaaa cgcgcaccca gttcatcttc
     2461 gagtttcgat acctgttgcg aaagagacgg ctgggcgaca tggcagcgct ccgcagcgcg
     2521 gctgaagctg ccggtttcgg caatagcgca aatgtatcgt agttgatgaa gctccataga
     2581 taacgtctat agtttatatc tcattcatag acgtaatcct tcgggcttcc cgcggggggc
     2641 cggctcatgg atttgctttc actgcactta ccgcaggaga ggccagcaca gacctgcctg
     2701 tccggcgtgc ccatacaatc aggagaatgc ctgcaagcac cgatccgaga ctggccatct
     2761 gggcgttgct cattccccag taaattttgg gattgatacg gacgaactcc accaggaaac
     2821 gcgcgacgcc gctcaaaata agatattccc ctgtaatctg gcctgccgga tgcgccgctt
     2881 ccggccgtcc acgtttccag agaaaccagg caatcagcag cgccacaatc aattcgtaaa
     2941 tgggtgtggg atgcacgcgc actccgggag gcgtgggcac caggccattc ggaaaactca
     3001 tgccccaagg caggtttgta ggaattccgt aatcgccatc tccggaagtg aggcaaccca
     3061 gacggcccac tccataacct acagccgctg cgggagcgga gaggtccagc atccccaact
     3121 tacttaaacc agcacctctg ctttgccaca gaagcgacag gattcccagt acaagcccgc
     3181 caaaccatgc gaacccggcg cggtccagaa gcataccgaa agggtcttgc cgcagaagct
     3241 gcgggctttc gagaacatgc cacagcttgg cgccgatgac tccggcaaca gtggaaatcg
     3301 caacaattcc aattgcatct gcgtctatct tccagcggcg aaagttgcgg tgcagtaccc
     3361 agcaggcgca gactgccgcc aaccagagca tgatgccaaa agtgccgatg gaaaaccggc
     3421 caatgtggat aaaggggtac acgtaattga gtatagcggg ctattctaaa tcagtgatga
     3481 gcacgcaaac cacgcccgcc cttgaggtcc ttttcagccg cgaacaaatt gcgcagcgcg
     3541 ttgccgaact gggccgccag attgaccagg actatgccgg acagaacatc gttttgctgg
     3601 gagtgttgaa gggcgcggcg atttttctcg ccgaccttgc gcggaacatc actgtgaaca
     3661 atacatttga ttttgttgca gtctccagct acggcaaggg ccagaaaacc agcggcgcgg
     3721 tcaggctcat caaagacgta gaccagccta tagaaggtaa aaacgttatt gttgtagaag
     3781 acattctcga taccgggctt acgctcgatt tcctcagaaa gctcttccag cagcataacc
     3841 cgcgttcgct gcgtatagcc gcgctgctcg acaagccgtc gcgacggttg gtgaaaattg
     3901 aagctgatta tgtaggattc agcatcccga acaaatttgt cgtaggctac ggcatggatt
     3961 atgccgagcg ttttcgtaat ctgcctgata tttgcctgat gccgccggat ttccctgaat
     4021 aggcatcctg gagtttgtaa ttaagatgac gaccaaagtt gtatctattg aagaagaatt
     4081 cagtaacgga ttttttgata ttgctgcatt cacacaccat gcgttttcca gcagaagcaa
     4141 tctcgcctca atattcgacc ataccctgct taagccagac gctacccgcg accaggtcgt
     4201 ccggctttgc gaagaggcgg cgcaatatgg ctttgcctgt gccatggtca acccggcatg
     4261 ggtgagcacg gcgtgctccg tactgtccgg aaccggaatt cgcgtaggca ccgtgctcgg
     4321 tttcccgctt ggggcctcgc tttccattac gaaacgggaa gaagctgtcg aactcatcaa
     4381 gctcggtgcg catgacctgg acatggtgct gaatatcggc ctgctcaaat ccggcatgaa
     4441 tgaccgcgtc cagcaggaca tccatggcgt agtagaagta gcacatgaag caggcgcaat
     4501 tgtgaaagtc atccttgaaa cgtgcctgct gaacgtcgag gaaaagctgc gcgcctctga
     4561 ggtggccatc gctgcaggcg cggacttcct caaaaccagt acagggtttt ccaccggagg
     4621 cgccactcct gaagatgttg ccctgctgcg cggcgtcgcc ggcggccgct ccggagtaaa
     4681 ggcctcggga ggcatacgga cacttgcgga aacgcgcgcc atgctcgaag ccggggcgaa
     4741 ccgcataggg gccagcgcgt ctgtgagcat tctgcgcgaa tttgacgcac tgtaagcagg
     4801 aggaacccat ttctacatcc atgcggcgtg acagcatacc attgtgccgc gatatcatgg
     4861 ctcctgtact ttcttaattg gggcgtgtca ggagtttcgt gagtaaagag caagtcaggg
     4921 ccaggaccgg cgacgaggcg cgccccgaag accacgcctt cgagggcgat taccgtccgc
     4981 cttcccccat tccggccaaa acccaggtgc gggtcgagcc gctgcagacc gagttcctgg
     5041 tgcgccttgc cgacgcactc aacacaacgc tcgacctgca aacgctgatg cagcggacgg
     5101 cagacatggt ccgggcggtc attgattacc gcatctttgc catcctgctg ctcaatgaca
     5161 ggaccaatga actgcgtatg cggttccaga tcggccatac acccgaggct gagcgcatgc
     5221 gcattcgcat gggaaagggc attgttggcc aggcagcaca gacccgcgaa cctgtgctgg
     5281 ttccggatgt aacgaagaca gagaactata tcaacgtcaa tcccgatgtg cgttcggagt
     5341 tggcggtacc gctcatcgtg aagaaccggg tgattggcgt gattgatatc cagtccgagc
     5401 agcccgatta ttttcagcag gagcacctgc atcttcttaa gctcaccgcc tcgcgtatcg
     5461 ggcaggcgat tgaaaacgcc cgcctttata cgcgcgtcgc gcgccaggcg cagatgctcc
     5521 aggtattgaa tgaaatcagc cgcgaaatca cctcgattct tgacctcgaa tcactgctgg
     5581 agcgtattgg aacactgctg cgccgcatca ttgattacca gatgttctcc atctggattg
     5641 tcaacgagcg cgatcatgtg ctggagaacc gcttcgcgct gcgctttggc gaacgcttct
     5701 accccgatga aaagcttcct ctcgaccagg gactcgttgg ggcagccatt gtcgagcggc
     5761 gcctgatcca tgcgcctgac gtccgcaagg acccacgata tcgcatgttt aatccggaga
     5821 cgcgctcgga gatggccatg cctttgatct acaaaggcaa ggtcatcggt attcttgata
     5881 tcgagcacac acggccacat ttctttaacg aagaccatga gcgcgccatt actactcttg
     5941 ccgctcaggt ggccatttca atcgagaatg cgcgtctcta tcagcgagtg gcccagcagg
     6001 aacagcgtct ggaagacgac cttgccatgg cccgcgaagt acagcttcgc ctgttgccat
     6061 cagtcaaacc aaagcatgcg cgcgctgagt tctctgcacg gtttttgccg gcccggacgt
     6121 taggaggcga tctttatgac tttatctcct atgacggaaa ccgcagcgcc attgtgcttg
     6181 gagacgtaag cggcaaggcc gctcctgctg ccctctatgc ggcccttgta agcggcatta
     6241 tgcgttcaga agcgacacag aagctctcgc cctcagagat gctgaagacc atcaacgatg
     6301 ctcttcagga acgccatgtg gattcgcagt acgtcaccat gctgtatgcc gtatggaatg
     6361 acgaaaacct cacgctccag ctatccaatg ccggggccgt gcagccactc ttctgtcgcg
     6421 acggcgaagt agaaaccgtg aaggccgagg gatttccgct tggactcttt ccaaatgtga
     6481 agtacgaaga attcactctc tccacccagc ccggagactc tctcatcttt tactccgatg
     6541 gcatcgtgga cgcgcagaat gccgtaggcg acatgttcgg aagtgaacgc ctcgcagccg
     6601 tcgtccaaaa gaacatgcat aagtcggctt caaagatcgc agatgccatt atgagcgagg
     6661 taggaaagtt ccagaaaggc gtagagcgct tcgacgatga aaccgtcgtt gtgttgaagg
     6721 tgcttggcgg aaaataggac catccgctgg cgaaagtcat ggcatttgtt ccttgacctg
     6781 ctcacctgaa atccgtacac ttatattttg cggcggatgc ggcaagcgtc tcttctgcct
     6841 gcatcccatt tccaaaggaa aattaaggaa gcatcatggc gaaccatgtt tcggcgctca
     6901 aacgcgcccg tcagacagag agaaagaccg cggtcaaccg cgccaacaag agccgtgtcc
     6961 gcggtgccat ccgctctctc cgcgaagctc ttgccaaggg cgatgt
//
LOCUS       DASRBV010000136         4556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_311349, whole genome shotgun sequence.
ACCESSION   DASRBV010000136 DASRBV010000000
VERSION     DASRBV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311349"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFA02_05225"
     CDS             <1..195
                     /locus_tag="VFA02_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09284.1"
                     /translation="ARQTAKTECPDSSVMIEAPHGKLKPEVRILYLWPEAGRLEAILA
                     QVRLISAGKKPAISAAFYSP"
     gene            529..1395
                     /locus_tag="VFA02_05230"
     CDS             529..1395
                     /locus_tag="VFA02_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09285.1"
                     /translation="MDALPLLIAQAGLSAAMRKAGITSQPRLLKNTGSNNDRWSWKIM
                     VSQIKPLSADGKSHNPSRPAIRVALADSQVIYRVGMRKIFATEPDIEVVAQFDNLVDL
                     QASLADHQPDVLILEGALATSQTVPEILAHSPGTKIIVQAAAMDEARTVELYRGGARG
                     IIPRAISPDLLVKCVRKVFEGETWIDNKAVAWVIEAYRSQAAGHAGIKGQPRLSPKEL
                     MIIHYITQGKRNKEIASELGTSEQVIKNYLRKIYDKLGVSDRLELALYSLNHKLHLKG
                     APVEANTGSDSR"
     gene            complement(1408..1878)
                     /locus_tag="VFA02_05235"
     CDS             complement(1408..1878)
                     /locus_tag="VFA02_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU09286.1"
                     /translation="MLHTRSATVQDAAIITHHRHRMFVDAGRKDNQVLHVMSQHFEPW
                     VRHMITEGKYLGWLTEEDGKVVAGAGLLLLDWPPHPLDPRSTHRGYLLNVYVEPEYRR
                     RKLASHLIEYALAEARRRKIRVVALHSTDEGRPLYESNGFRPTNEMFYVEPVEP"
     gene            1909..3282
                     /gene="tilS"
                     /locus_tag="VFA02_05240"
     CDS             1909..3282
                     /gene="tilS"
                     /locus_tag="VFA02_05240"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZU09287.1"
                     /translation="MSQAKTNPLPLLTGCLHPGMRLGVAVSGGADSVALLRVLARRRQ
                     ELGLVLTALHVHHGIRGAEADGDASFAEGLAKDLGVRFLRNDVNTPERAQKAGESIEE
                     AARNLRYAWFDELLGQGELDAVATAHTLDDQAETVLLKLLRGAWTEGLAGIFPVLKRE
                     KGAIVRPFLETRRSEIQAWLREVGQSWREDSTNQDVVYARNRVRHHLLPILEEYNPQI
                     ASHLACLSEIARDEEAYWQAELARILPSLLLPGKPVRGGGRAISTHPEQASVGMEVER
                     LRGVHPAMRRRVLRAAAEQLGACLNFDQTELLLELCEGKAGRRQVLTAELRAERSPRE
                     LRLVRSASAKADAPPEYEFTVPGQVVAEAFGLRLTVSVLAEEFSGSTARLRAYRSGDR
                     VQLRYSSGPKRVKEVLERMQVPSAERAGWPVLEWEGEIVWMKGAEVESKAGSRAGLKV
                     VMQESRT"
     gene            3338..3580
                     /locus_tag="VFA02_05245"
     CDS             3338..3580
                     /locus_tag="VFA02_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09288.1"
                     /translation="MRTTVNIDADAYDYAYCYAYGKGISLGKAISELIRKAEKAPQPE
                     MSSRLKLNEHGYYEIVGPGDPITIEDVKKYSEDPIE"
     gene            3582..4007
                     /locus_tag="VFA02_05250"
     CDS             3582..4007
                     /locus_tag="VFA02_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZU09289.1"
                     /translation="MRYLLDVNTLIALIFPLHVHHSVVKPWFNADPFLSWAICPFTEA
                     GFLRYAMAPHLGQVKLEEATIALEKLAKHPGYHYLPAIAEWRSLCQPFFQRLFGHKQI
                     TDAYLLGLAVRENYKLVTLDRAILHLAGEYQDHVLFLGE"
     gene            4194..>4556
                     /locus_tag="VFA02_05255"
     CDS             4194..>4556
                     /locus_tag="VFA02_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HZU09290.1"
                     /translation="MNSTVKTIIFWVFVFACLVLLWQVFEKGTGSGKEQEITFSQFLQ
                     DAQQGQLSDVTVMGSEVRGHYKNDKSQFHVTVPANYPHLYDILSENKVSVTVKDQNGS
                     SWLPIIINFAPIIIIGLLW"
BASE COUNT         1081 a         1091 c         1342 g         1042 t
ORIGIN      
        1 gccaggcaga cggcgaaaac agaatgtccg gactcttccg tgatgatcga agcgcctcac
       61 ggaaagctaa agcctgaagt ccgaatactt tatctctggc cagaagcggg acgcctggaa
      121 gcaattttgg cccaggtccg cctgatttcg gcaggcaaaa agccagcgat ttccgcggcc
      181 ttttactctc cataaataaa tcaggttctc ccaggcaaca aaaaatattt ttccgcaccc
      241 tttagttacg ccttagactt agactttagt aatcgagggc ggttgttttg agtgggcata
      301 ttccaaaatt ggaaagcgag aacggcaaag aaggaggcga ggaaggattt cgctcctttg
      361 cccgctatgc tttgaatttg cccgtccgca tcctatcagg aacagaggag tttcacgcaa
      421 tcacggaaaa tatttccgca aatggagtgt tgttccgcct ggagaagtgc cttcctgtag
      481 ataccccgat agagtttttg atggaaattc cagaaaaagc attgggaaat ggatgcgctg
      541 ccgctgctca ttgcgcaggc aggattgtcc gcagctatga ggaaggccgg aattacttcg
      601 cagccgcgat tattgaagaa tacaggttcc aataacgata gatggtcatg gaagattatg
      661 gtgtcacaaa taaaacctct ttctgccgat ggtaaaagtc acaacccgtc caggccggcc
      721 atacgcgtgg ccctggcaga ttcacaagtc atctaccgcg tgggcatgcg gaagattttc
      781 gccacagagc ctgatatcga agtagtcgcg caatttgaca atcttgtgga cctgcaggct
      841 tctttggccg accatcagcc cgatgttctg attctggaag gagcactggc aaccagccag
      901 actgtgcctg agattctggc ccattctccg ggaaccaaaa ttattgtgca ggcggcagcg
      961 atggacgaag cccggactgt ggagctttat cgcgggggtg cccggggaat tattcctcgc
     1021 gcgatttctc cggatttgct ggtgaagtgc gtgcgtaaag tatttgaagg cgagacctgg
     1081 attgacaaca aagcagtggc atgggtgatt gaggcgtatc gctctcaggc cgccggtcat
     1141 gcaggtatca aaggccagcc caggctgtct ccaaaagaat tgatgatcat ccattacatc
     1201 acccagggca agcgcaataa ggaaatcgcg agtgaactgg gaacaagtga gcaggtcatc
     1261 aaaaactatt taaggaaaat ttacgacaag cttggagttt ccgacaggtt ggaactggcg
     1321 ctttattcac tgaaccataa gctgcacctg aaaggtgcgc cggttgaggc aaacactggt
     1381 tcggattccc gatagcaagg gttgcaatca cggctctact ggttccacat aaaacatttc
     1441 gtttgtagga cggaagccat tgctttcata aagcggacgg ccttcatccg tggagtgcaa
     1501 cgccacgaca cggattttcc gccggcgggc ttcagccaga gcatattcga tgaggtggct
     1561 ggccagtttg cggcggcggt attccggctc aacatagacg ttcaacagat agccgcggtg
     1621 cgttgagcga ggatcaagcg gatgtggcgg ccagtcgaga agaagcagac ctgcgcccgc
     1681 gactaccttt ccgtcctctt ctgtaagcca gccgagatac ttgccttccg tgatcatgtg
     1741 cctgacccac ggttcaaaat gctgagacat cacatgcagc acctgattgt ctttacggcc
     1801 tgcatctaca aacatgcgat ggcggtgatg ggtgatgatg gcagcatcct gaactgtggc
     1861 ggagcgggtg tgtagcatac gcagccagta taggatgtct ttctgagtat gtctcaagct
     1921 aaaacgaatc cgttgccgct cctgactggc tgtctgcatc ccggaatgcg cctgggagtg
     1981 gctgtttctg gtggtgcgga ctccgtggcg ctgctgcggg tgctggcacg gcgaaggcaa
     2041 gaactaggcc tggtgctgac ggcgcttcat gtgcatcatg gtattcgcgg cgctgaagcg
     2101 gatggagatg cgtcgtttgc agagggcctg gcgaaggatt tgggggtccg ttttctgcga
     2161 aacgatgtaa atactccgga gcgtgcgcaa aaggcaggag agagcatcga agaggccgca
     2221 cgcaacctgc gctatgcatg gtttgacgaa ctccttgggc aaggcgagct ggatgcggtg
     2281 gcgaccgcgc acactctcga tgaccaggcc gagactgttc tgttgaaact gcttcgcgga
     2341 gcatggacgg aagggcttgc tggaattttt cctgttctga aacgtgaaaa gggggcgatt
     2401 gtccggccat ttctggaaac gcggaggtcg gaaattcagg cttggttgcg cgaggttggt
     2461 caatcgtggc gtgaagattc cacaaaccag gatgtggtct atgcccgcaa cagagtccgc
     2521 caccatctgt tgcccattct cgaagaatat aatccgcaga ttgcttcaca tctggcctgt
     2581 ctgtcagaaa tcgcccgtga cgaggaggcc tattggcaag ctgagctggc gaggattttg
     2641 ccgtcactac tcttgcccgg aaagccggtg cgcggcggag gccgggccat ctccacccat
     2701 ccggaacagg cttctgtagg aatggaagtg gagcgcctgc gcggagtgca tccggcgatg
     2761 cggcgcagag tgcttcgagc agcagccgaa caactggggg cttgcctgaa ttttgaccag
     2821 acagagctgc ttctggagtt gtgcgaaggc aaggccgggc gacggcaggt tctgacggct
     2881 gagcttagag cagaacgttc cccgcgggaa ctccgccttg ttcgcagcgc ttccgcaaaa
     2941 gctgatgccc cgcccgaata tgaatttaca gtccctgggc aggtggtagc ggaggctttc
     3001 gggctacggt tgactgtgtc ggttttggcg gaggagtttt ccggatctac tgcgcggttg
     3061 cgggcttata ggtccggaga ccgcgtccag cttcgttaca gcagcggccc caagcgggtg
     3121 aaagaagttc tggagcggat gcaggtccct tcggcagagc gggcaggctg gcccgtcctc
     3181 gaatgggagg gtgagattgt ctggatgaaa ggggcagaag tcgagagcaa ggcaggaagc
     3241 agagccggat tgaaagtcgt gatgcaagaa agccgaacgt gaaggcgcat caaagttcat
     3301 atcggcatca atataagaaa tgatgtaaaa tatgaatatg agaacaacgg tcaacattga
     3361 tgctgatgct tacgactatg cgtattgcta cgcgtatgga aagggaatct ctctggggaa
     3421 ggccatcagt gagcttatcc gtaaggcaga aaaggccccg cagcctgaga tgtcttccag
     3481 gctgaagctg aatgagcacg ggtactatga gattgttgga ccaggcgacc ccatcactat
     3541 tgaggatgtg aaaaagtact cggaggaccc gattgaatag ggtgagatac ctccttgatg
     3601 taaatacgct gattgcctta atttttccgc ttcatgtaca tcactcagtc gtaaaaccct
     3661 ggtttaacgc agatccattt ctttcgtggg caatttgccc ttttactgaa gcgggctttc
     3721 ttcgttatgc gatggcgcct catcttgggc aggtgaagct tgaagaagca acgattgcac
     3781 tggaaaaact tgcgaaacat cctggctatc attacctgcc tgcaatagcg gagtggcgta
     3841 gcttatgcca gccgttcttt cagcgtttgt ttgggcacaa acagatcaca gatgcgtatc
     3901 tactggggct tgcagtgcgc gaaaactata aattggtgac ccttgacagg gccattctgc
     3961 atctggccgg cgaataccag gaccatgtcc tgtttctggg cgagtaagag taaaaccgtt
     4021 ccgctttcgc cgtatctatt tccgctgatg cgggtttcaa gagagtagaa tgattgcaac
     4081 gctcttcgag ggagggcgta aggacgtttt tgcggcactt ttggccgggg aaaagcgtct
     4141 aatcagaagg cgcgcctgcg cgagggctgg cgtgtagaaa aagaggattt ttcgtgaatt
     4201 caacggtcaa gaccatcatc ttctgggtgt ttgtcttcgc gtgcctggtg ctgctgtggc
     4261 aggtattcga gaagggcacg ggcagcggca aggaacagga gatcaccttt tcacaatttc
     4321 tgcaggacgc ccagcaggga cagttgagcg atgtcaccgt catgggcagt gaagtgcgcg
     4381 ggcactataa gaacgacaag agtcagttcc acgtaacggt ccctgcaaat tacccgcatc
     4441 tgtacgacat tctttcagag aacaaagtgt ctgtgaccgt aaaggaccag aacggaagct
     4501 cgtggctgcc gatcattatc aactttgcgc cgatcatcat tattggcttg ctgtgg
//
LOCUS       DASRBV010000137         9152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_670572, whole genome shotgun sequence.
ACCESSION   DASRBV010000137 DASRBV010000000
VERSION     DASRBV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670572"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="VFA02_05260"
     CDS             complement(<1..1982)
                     /locus_tag="VFA02_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZU09291.1"
                     /translation="MNFRSSAICLSVSALTVSLWAQTQPQSLFGYRDFSAQARIDQQF
                     LAVPNAKLAGEELKTLTAAPHIAASKEDHDTALYVASKFKAAGLETEIVPYKVWLNLP
                     RKISVTATDSAGKPLMTGPTREHVEGDPFDKDPRVVTAFNGSSPSGDVTAEVVYANYG
                     RPEDFKKLDDLGISVKGKIVIVRYGQNFRGVKVYIAQQRGAAGVIIYSDPADDGYYRG
                     DMYPNGPWRPETGVQRGAVQYLFQYSGDASTPGIAARPDLPDSQRINPATAPNMPQIP
                     STPLSYHDAAPILKALAGPSVPQGWQGALPFTYHVGPGGVKVHMVLQQDYAYRTIWDV
                     IGKVKGTEWPDEWVIAGNHRDAWVYGAVDPNSGTAAMLEAVHGIGQLLKSGWKPRRTL
                     IFASWDAEEEGLIGSTEWAEDNAAALRHAVAYFNTDVGVAGPNFDASAVPSLKQFIRE
                     IAKEVPSPHGGSVYDRWKKSEAEESSRRRLMNEHASHPAAPESDVHVGDLGSGSDYTP
                     FIQHLGVPSTDIGSAGPYGVYHSVFDNYNWFIKNADPTFVFEQQQARIFGLEALHMAD
                     ADVLPYDYVTYGKEITEYLNAAKKKADDEKVSGLDFSPALSAAQRFTAAAEAVYKKQQ
                     SIPSDLPQFNTQLRQVESDLLSSAGLPNRPWFKHTI"
     gene            2210..3502
                     /locus_tag="VFA02_05265"
     CDS             2210..3502
                     /locus_tag="VFA02_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU09292.1"
                     /translation="MAAKESAPVVLDPELKNDLVMDLPVAGKTWPAIVRALRNDDYRL
                     FWGGCLLSNIGTWMQNVAQGWLVLELSNSSFWLGMVGFAASFPFLLFTLFGGVIADRV
                     SKRKLLMGTQAAQMIFAFVLALLTYFKVITIRQLVLIAFCNGIANALNAPTYQAMVPR
                     LVPPEDLPNAIALNSAQFNMSRILGPTLGGYAMAWIGMAGNFFLNAVSFLAVLWPLHR
                     MRYPEEIQERHGSVLKSLRDGIDYVRKSAQMSAVVLLIMSASLLLLPFITFIPYFAKD
                     VLHAGERGLGFLMACSGIGALTGAGIIAYLGKIRWRGRVIVFAGLFVMAAVLVLCYSK
                     VFALSAAMCFCEGLGMIISLSTVNVTMQQLSSDAMRGRVMSIYAASFLGLPPVGCLIV
                     GEMSRHILTEHAIAILTGLAMVCYVGFYAASKELRQLD"
     gene            complement(3571..5700)
                     /locus_tag="VFA02_05270"
     CDS             complement(3571..5700)
                     /locus_tag="VFA02_05270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZU09293.1"
                     /translation="MGRVRNFEARVFHAVRREPPQSKVHRLAFWLLMIYLALIPERLL
                     PGMAGEFFNGISIFTLVLLVIFCVPLFFRWIFGRFLWKLRNRLILTYLLMALTPVVLF
                     VMLALISLYVFSGQFAIFAASAEMNTQLEHFAAENRAFSMHVAHVLAQQPSAKTVALP
                     EMVDTSPDSPDAGLQVAAFYDGRPVAVTPQNLNAGYITQVPDWVKHGFEKGVAWDRNK
                     FWLRAVDTQTINGHTTTLISSIPLDKSTIDQVAHGIGRIEIIPVLQEEPSSAGQSQPA
                     QSSRQSVENELSRRKRVREEIRENSISGGTLPPGQHFYDIPITFYAPLEPRDWSTGKL
                     HDTSVIVTSRPSLLYNRLFITSLRIATVIQDILIGVAIFFGLLELIAFIMAIRLNRTI
                     TRSVNDLYEATIEIDKGNLAHRIKVTRNDQLAALSRSFNRMSYSLERLLAEQREKERL
                     QSELSIAQEVQANLFPRGAISLPMLELHGACYPARTVSGDYYDFLVFGDSQLGLALGD
                     ISGKGISAALLMATLHSAVRAYRFAGEDLLVTARHASSNGNGNGDPFLEPARILTQLN
                     RHLYRSTQPEKYATLFLAHYDGTNQQLTYSCGGQLPPLVLRADDTVATLACGGTVVGL
                     LENATYDQGVEQLHPGDILIAYSDGVTEPENDFGEFGEDRLLEVVRRHRHKPLDAISE
                     QVMQALRNWIGGQEQPDDITLVLARQK"
     gene            5898..7853
                     /locus_tag="VFA02_05275"
     CDS             5898..7853
                     /locus_tag="VFA02_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZU09294.1"
                     /translation="MIRFLQKDSRIVKWIFIVIIAIACVTMVITLVPGIFSNTETSSD
                     TYATIRGGGIWGRFFGSGDQVSMQDVQMLAQRMMQRQQLPDFVMPYMVQRVGQGLIQQ
                     HIELEEAGRLGIQVTDDDLRNFLHTGMWGQVLFPNGQYIGDERYASLVSENFGISREK
                     FESEVKKELLEQRLRSFVTAGVTVSDQQVRDAYTQQATRIKFQYAVLSSDDLRKQINP
                     TDTELQTFFKQNAARYAKAIPEKRRIEYIAFTTANLPGGVPQVTDAEVQQYYSQHQSN
                     YHVDDQVKVRHILIKVDPNADAKADAAAKQKAEDILKQLHNGGNFAELAKKYSDDPGS
                     KDQGGELGFIKHGETVPEFDKTAFALQPGQMSDLVRTKYGYHIIQAEEKQAAHTRPLD
                     EVKPEIVALLTRQKEAQAEQAFASQLAQEAQKNGLDKTAQAHHLQAVTTDYLEQNAVV
                     PGLADGSQLLTSAFSAKPGSAPLVASTGDGYAVFQVKDVQAAHAPTFEEYKSHILDDY
                     REEQIPQLLARKTNELADKAHAENDLEKAAKEVGATVKTSDLVGRDAQVPDIGQLASV
                     APTLFDLNTGQVSKAINTGRTGVVAKLLDKQLPTPDDIQKHFESTRDALLDQQREQMF
                     EVFLSNLMAKYEKEGRIRINRRAQQQS"
     gene            7933..>9152
                     /gene="malQ"
                     /locus_tag="VFA02_05280"
     CDS             7933..>9152
                     /gene="malQ"
                     /locus_tag="VFA02_05280"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZU09295.1"
                     /translation="MPFKRASGLLLHITSLPSYGGTGDLGPAAYKFADFLAAAKQRLW
                     QVLPLSPVGYGNSPYAALSAFAGNPLLISLEKLSDWGWIEGKRIAGLPGRSGNVHFDE
                     VAQKKVPLLREAARNFLHNHGHLQEQWSRFEGYCGINGSWLNDFVRFTVLRRKFDFAS
                     WNTWPQEFARRNPEALARFDQEHAEELAIERIIQFAFDEQWAALRQYCAARSIRFIGD
                     VAIFVSYDSADVWTHPDIFELKEDLSPLRVAGVPPDYFSKTGQRWGNPLYRWSVLEQR
                     GFDWWVDRIRRALALYDMVRLDHFRGFEAYWSIPAEEETAVNGEWVKAPGSALFSKLK
                     EALGELPFIAEDLGVITPEVDALREQFGFPGMRVLQFGFSNRGAHNYLPHRFEKNAVV
                     YTGTHDNDTTRGWW"
BASE COUNT         1942 a         2348 c         2861 g         2001 t
ORIGIN      
        1 tagatggtgt gcttaaacca cgggcgattg ggcagtcctg cggaagaaag caggtcactt
       61 tctacctggc ggagctgcgt gttgaactgc ggcaagtccg atgggatgct ttgctgtttc
      121 ttatagacgg cctcggcggc cgcggtaaat cgctgcgcgg ccgagagcgc cggggagaaa
      181 tcgagaccag agactttttc atcgtcggcc ttcttctttg ccgcattcag gtattctgtg
      241 atttctttcc cgtaggttac atagtcatag ggaaggacgt cggcatcagc catgtgcagg
      301 gcctcaaggc caaagatgcg ggcctgctgc tgctcgaaga cgaaggtggg atcggcgttt
      361 ttgatgaacc agttgtagtt atcaaagacg gagtggtaga ccccataggg gcctgcggag
      421 ccgatgtcgg tagagggtac gccgaggtgc tggataaaag gagtgtagtc ggatccactg
      481 ccgaggtcgc caacatgaac atcgctttcg ggcgccgcag gatgggaggc gtgttcgttc
      541 atcaaccggc gacgggatga ttcttccgct tcgcttttct tccaccggtc gtagacagaa
      601 ccgccgtggg ggctggggac ttccttcgca atttcgcgga tgaactgttt gagcgagggc
      661 acggcggagg catcgaagtt cggaccagcc actcctacat ctgtgttgaa ataagcaacg
      721 gcatgccgga gggcggcagc gttgtcttcg gcccattcag tagagccgat gaggccctcc
      781 tcttcggcgt cccaactggc aaagatgagg gtgcgcctgg gtttccatcc ggacttgagc
      841 aattgcccga tgccgtggac ggcctccagc atggcagcgg tcccgctgtt ggggtccact
      901 gcgccgtaga cccaggcgtc gcggtggttt ccggcaatga cccattcgtc cggccattct
      961 gtgcctttga ctttgccgat gacgtcccag atggtgcggt aggcgtagtc ctgctgaagg
     1021 accatgtgga cttttactcc gccagggccg acgtgatagg tgaaggggag cgcaccctgc
     1081 catccctggg gcacggaggg gcctgcaagg gccttcagaa tgggcgctgc atcatgatag
     1141 gagagcggtg tggaggggat ttgcggcata ttgggcgctg tggcagggtt gatgcgctgg
     1201 gaatccggaa gatcaggccg ggcggcaatg ccgggagtgc tggcgtcgcc cgagtattgg
     1261 aagagatact gcacggcgcc gcgctggacc ccggtttccg gacgccaggg gccgttcggg
     1321 tacatatcgc cgcgatagta tccgtcatct gccgggtcag aatagatgat gactccggca
     1381 gctccgcgct gctgcgcgat gtagaccttc acgccgcgga agttctgtcc gtaacggacg
     1441 atgacgattt tgcctttcac gctgatgccg aggtcgtcga gttttttgaa gtcttcaggg
     1501 cggccgtagt ttgcgtagac cacttcggcg gtcacatctc cagaggggga agagccgttg
     1561 aaggcagtga cgacacgcgg gtctttatca aacgggtcgc cttcgacgtg ctcgcgcgtg
     1621 gggccggtca tgaggggttt tcccgcggag tcggtggcag tcacgctgat ttttctgggc
     1681 agattgagcc agaccttgta ggggacgatt tctgtttcca gccctgcggc tttgaacttg
     1741 ctggcgacat acagggcggt gtcgtggtct tcctttgagg cggcaatgtg cggagctgcc
     1801 gtgagtgttt tcagttcttc tccggcgagc tttgcgttgg gcacggcaag gaattgctgg
     1861 tcaattctgg cctgggcgga gaagtcacgg taaccgaaga gggactgagg ctgcgtctgc
     1921 gcccagagtg aaacagtgag ggccgaaacg gaaaggcaga tggcggaaga gcgaaagttc
     1981 aagtatctgc tccttgaagg acattcaaaa tgccgatgaa acagtcagct tacactatct
     2041 gagagggaag cggcggaaga agtacgcaga ttgtcgcatt ttcggcacgt gtgggcattc
     2101 ttacatgtct tgtcaggaaa ttggcagtct ataaaacatt actcttgaag agatggcagg
     2161 acggaatgtg tcccggtaaa cgatgtagaa acgacgagag gagcgcgcct tggctgcgaa
     2221 ggaaagcgct ccggtagtgc ttgatccgga actgaaaaac gatctggtga tggacctgcc
     2281 agtggcgggc aagacatggc ctgcgattgt gcgggcgctc cgcaacgatg attaccgcct
     2341 cttctggggc ggatgcctgc tttccaatat tggcacctgg atgcagaacg tggcccaggg
     2401 ctggctcgtc cttgaacttt ccaattcatc tttttggctg gggatggtgg gatttgccgc
     2461 atccttcccg tttttgctgt tcacgctttt tggcggagtg attgccgacc gcgtaagcaa
     2521 gcgcaagctg ctgatgggta cgcaggcagc gcagatgatt ttcgcgtttg tgctggcgct
     2581 gcttacctat ttcaaagtca ttaccatccg gcagcttgtt ctgattgcct tttgcaatgg
     2641 cattgccaat gcgctgaatg cgccgacata tcaggcgatg gtgccgaggc tcgttccgcc
     2701 ggaagacctg cccaacgcca ttgcgctgaa ctcggcgcag ttcaacatgt cgcgcattct
     2761 cgggccgacg ctgggcggtt acgcgatggc gtggatcggc atggccggaa atttctttct
     2821 gaacgccgtc agctttctgg cagtgctgtg gccgctgcac aggatgcgct atccggaaga
     2881 gatacaggag cggcatggga gcgtattgaa gagtctgcgc gacggaattg attatgtgcg
     2941 gaagtcggcg cagatgtcgg cggtggtgct gctgattatg agcgccagcc tgctgctgct
     3001 gccgtttatc acttttattc cgtattttgc gaaggacgtt ctccatgcgg gtgagcgcgg
     3061 gctgggcttt ttgatggcct gctcggggat tggagcgctg accggggcag gaatcattgc
     3121 atatctggga aagatacgct ggcgtgggcg ggtgattgtc tttgccggac tgtttgtgat
     3181 ggcggcggtg ctggtgcttt gctactcgaa agtatttgcg ctttcggcgg cgatgtgctt
     3241 ctgcgagggc ctggggatga ttatcagcct ctcgacggtg aacgtcacga tgcagcagct
     3301 ttcgagcgac gccatgcgcg ggcgggtgat gagcatttac gcagcgagct ttctgggact
     3361 gcctccggtg gggtgcctga ttgtgggcga gatgtcacgc catattctga cagaacacgc
     3421 aattgcgatt ctgaccgggt tggccatggt gtgttacgtg gggttttatg cggcgtcgaa
     3481 ggaattgcgg cagttggatt agattttctt ctgcgggcaa tcctaaaatg cgcagtccgc
     3541 aaaaggccgg gcactctttc ctgattgcat ttatttctgg cgggcgagga cgagggtgat
     3601 gtcgtcgggt tgttcctggc cgccgatcca gttacgcagc gcctgcatga cctgctctga
     3661 gatagcatcg agcggcttgt ggcgatggcg gcgcacgact tcgagcaagc ggtcttctcc
     3721 aaattcgccg aagtcatttt ccggctcggt tacgccatcg ctgtaggcga tgaggatgtc
     3781 gcccggatgc agttgctcta caccctgatc ataggttgcg ttctccagaa ggccgacgac
     3841 ggtaccgcca caggccagag ttgccacggt atcatctgcg cgcaatacga gaggcggcag
     3901 ttgtccaccg caggaatacg tgagctgctg gttggttccg tcgtaatgtg ccaggaacag
     3961 ggttgcgtat ttttccggct gggtgctgcg gtacagatgc cggttgagtt gggtgaggat
     4021 cctggccggc tccagaaacg ggtcaccgtt cccattgcca ttgctgctgg catgtctggc
     4081 tgtgacgagc agatcttctc ctgcaaaccg gtaagcgcgc acggccgagt gcagggttgc
     4141 catgagcagg gccgcggaaa tccccttgcc gctgatgtct ccaagcgcca ggccaagctg
     4201 cgaatcacca aagacgagga agtcatagta atcgccactg acggtacgtg ccggatagca
     4261 tgcgccatgc agctcaagca tgggcaggga aatagcgccg cggggaaaga ggttggcctg
     4321 gacttcctga gcaatggaaa gctcgctttg caggcgttcc ttttcccgtt gttcggccag
     4381 aaggcgctcc agagaataag acatgcggtt gaaagaacgg ctgagggcgg cgagctggtc
     4441 gttgcgcgtg actttgatgc ggtgcgcgag attgcctttg tcaatctcta tcgtggcctc
     4501 atagaggtca ttgactgaac gcgtgatggt gcggttgagg cgtatggcca taatgaaggc
     4561 aatcagttcc agaaggccaa agaagatggc gaccccaatc agtatgtcct gtatgacggt
     4621 ggcaattctg agcgaggtaa taaacaggcg gttataaagc agcgatggcc gggatgtaac
     4681 aatcactgaa gtgtcgtgga gcttgcctgt gctccagtct ctgggttcca gcggagcata
     4741 aaaagtaatc gggatgtcgt aaaaatgctg gcctggtggg agcgttcctc cagagatgga
     4801 attttcgcgt atttcttccc gtacccgctt gcggcggcta agctcgtttt cgacagactg
     4861 gcgtgaagat tgggcgggtt gtgactggcc ggctgaagac ggttcttcct gaagaacggg
     4921 aatgatctca attctgccga tgccgtgcgc cacctggtca atggtggact tgtccagcgg
     4981 gatgctggaa ataagcgtag tggtgtgacc attgatggtc tgggtgtcaa cggcgcgcag
     5041 ccagaacttg tttctgtccc aggccacacc tttttcaaac ccgtgtttta cccaatcggg
     5101 tacctgggtg atgtagccgg cattcagatt ttgtggtgta acggcgacgg gacggccatc
     5161 ataaaaggcg gccacttgca ggccggcgtc cgggctgtcc ggtgaggtgt ctaccatttc
     5221 cggaagcgcg acggttttgg ctgaaggctg ctgggccaga acgtgcgcga cgtgcatgga
     5281 aaaggcgcgg ttctccgcgg caaagtgctc aagctgagtg ttcatctcgg ctgaagcggc
     5341 aaagatggcg aactgtccgc tgaaaacata cagcgagatg agggccagca tcacgaacag
     5401 gacaacgggc gtgagcgcca tcagcagata ggtcaggatg agccggttgc gcagcttcca
     5461 caggaaacgg ccgaagatcc agcgaaagaa gagtgggacg caaaaaatga ccaacagaac
     5521 aagggtgaag atgctgattc cgttgaagaa ctctccggcc attccgggca gcaggcgctc
     5581 cggaatgagg gcgaggtaga tcatcagcag ccagaaggcg aggcgatgca ccttgctttg
     5641 tggcggctcg cgtcttacgg catggaaaac gcgggcttca aaatttctga cccgtcccat
     5701 aaatggatag ctgatggaat tgagtgtatc gcaatggagc agtgtccgat ggcagtgccc
     5761 ggattgctca ggacggcgga gctttcccca tcaacgcgaa aggcatggag gacgggaaac
     5821 ccaaggcaaa ccatcctgac gctgatttat aatcaaacag atactcacgt tctcacagcc
     5881 aaaggccgta accattcatg attcgttttc ttcaaaaaga cagccgcata gtcaaatgga
     5941 tttttatcgt catcatcgct attgcctgcg tcacaatggt gatcacgctt gtacctggta
     6001 tcttcagcaa cacagagaca agctctgaca cctacgccac gatccgcggc gggggcatct
     6061 ggggccgctt ctttggttcg ggcgaccagg tttccatgca ggacgtgcag atgctcgcgc
     6121 agcgtatgat gcagcgtcag cagcttcccg attttgtgat gccctacatg gtgcagcgtg
     6181 tgggccaggg gctgatccaa cagcacattg aacttgaaga ggccgggcgc ctcggcattc
     6241 aggtgaccga tgatgacctg agaaactttt tacatacggg aatgtggggc caggtgctct
     6301 ttccgaacgg ccagtatatc ggcgacgagc gctatgccag tctggtttca gaaaacttcg
     6361 gcatttcgcg cgaaaagttt gagagcgaag taaagaaaga gctgcttgag caacggctgc
     6421 gctcttttgt tacggcggga gttacagttt cagaccagca ggtgcgcgat gcttacacgc
     6481 agcaggccac caggatcaag tttcagtatg cagtgctaag ctctgacgat ttgcgcaagc
     6541 agatcaatcc gacagatacc gagctacaaa ccttcttcaa acagaatgcc gcgcgctatg
     6601 caaaggccat tcctgaaaag cgcaggattg agtacattgc gtttaccacg gcgaacctgc
     6661 cgggcggcgt tccgcaggtg actgacgcgg aagtgcagca gtactacagc cagcaccaaa
     6721 gcaactacca cgtagacgac caggtgaagg tacgtcacat tcttatcaaa gttgacccga
     6781 acgctgatgc caaagccgat gcggccgcaa agcagaaggc cgaggacatc ctgaagcagc
     6841 tccataacgg agggaacttt gctgagctgg caaagaagta ctccgatgac ccaggcagca
     6901 aagaccaggg cggggaactt ggctttatca agcatggcga gactgtgccg gaatttgata
     6961 agacagcgtt cgcgctccag cccggacaga tgtccgacct cgtccgcact aagtacggat
     7021 accacatcat ccaggcggaa gaaaaacagg ctgcgcacac tcgcccgctt gatgaagtga
     7081 aaccggaaat tgtggccctg ctgacccgtc agaaagaagc gcaggccgag caggcctttg
     7141 ccagccagct tgcccaggaa gcgcagaaga acggtctgga caagaccgcg caggcgcacc
     7201 atcttcaagc tgtgaccacc gattatcttg aacagaacgc cgtagtcccg ggcctggctg
     7261 atggttcgca gttgctgacc agcgcatttt ctgcaaaacc cggttctgct ccgctggtgg
     7321 cctccaccgg cgatggatat gccgtgttcc aggtgaagga cgtacaggcg gcacatgctc
     7381 ctacgttcga ggaatacaag tcgcacatcc tggacgatta tcgcgaagag cagattccgc
     7441 agttgctggc gcggaagacc aacgagctgg cggacaaggc gcacgccgag aatgatctcg
     7501 aaaaggcggc caaggaagtc ggcgcaactg taaagacaag cgatctggtg ggacgggatg
     7561 cgcaggtgcc ggacataggc caactggcat cggtcgcacc tacactcttt gacctcaata
     7621 cagggcaggt cagcaaggcc atcaacaccg gacgcacagg ggtggtcgca aagctgctcg
     7681 ataagcaatt gccgacgccg gacgatatcc agaaacactt tgagagcaca cgcgatgcgc
     7741 tgctggacca gcagcgggag cagatgttcg aggtcttcct ttcaaatctt atggcaaaat
     7801 acgagaagga agggcgcatc cgcatcaacc ggcgcgcgca gcaacagtcg taaaactgaa
     7861 caaaagcaac taaggcccgt gaatgagaat ctctcgaacc gcgggccttt tctttcttta
     7921 aggaaaatct ttatgccatt taagcgtgct tccggactgc tgttgcacat tacctcactg
     7981 ccttcttatg gcggcaccgg cgatctgggg ccggccgcat ataagtttgc tgatttcctg
     8041 gctgccgcga aacaacgcct ctggcaggta ctgccgctga gtcccgttgg gtatggcaac
     8101 tcgccttacg cggcgctttc tgcctttgcg ggaaatccgc tgctgatcag cctggaaaag
     8161 ctctctgact ggggctggat cgagggcaaa cgcattgctg gcctgcccgg gcgatcagga
     8221 aacgtgcatt ttgatgaagt tgctcagaaa aaagtcccgt tgttgcgcga ggctgcgcgt
     8281 aacttcctgc acaaccacgg ccatcttcag gaacagtggt cgcgttttga aggttactgc
     8341 ggcatcaatg gttcctggtt gaacgatttt gtccgcttta ccgtgctgcg ccgcaaattt
     8401 gatttcgcaa gctggaacac ctggccgcag gagtttgcac ggcgcaatcc agaggccctt
     8461 gcgcgcttcg atcaggaaca tgccgaggag cttgccatcg aacgcatcat tcagtttgcc
     8521 tttgatgagc agtgggcggc gctgcgccag tactgtgctg cgcgcagcat ccgcttcatc
     8581 ggagacgtgg cgatctttgt gagttatgac agcgccgatg tgtggacgca ccccgacatc
     8641 ttcgagctga aggaagacct ttcgccgctg cgcgtggccg gtgtgccgcc ggactatttc
     8701 agcaaaacgg ggcagcgctg ggggaatcct ctctaccgct ggagcgttct ggagcagcgc
     8761 ggcttcgact ggtgggtaga ccgtatccgg cgcgcccttg cgctttatga catggtgcgg
     8821 ctggaccact tccgcggctt tgaggcgtac tggtctattc ctgccgaaga agagacggct
     8881 gtgaatggcg aatgggtaaa ggcacccgga tctgctctgt tttctaaact gaaagaagct
     8941 ctgggcgagc tgccatttat tgccgaagac ctcggcgtca tcacgccaga agtggatgcg
     9001 ctccgggaac aatttggctt ccccggaatg cgtgtcctcc agtttggctt ttccaatcgc
     9061 ggagcgcaca actatctgcc gcaccgcttt gagaagaacg cggtcgtcta cacgggaacg
     9121 cacgacaatg acacgacccg cggctggtgg ga
//
LOCUS       DASRBV010000138        12364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_565729, whole genome shotgun sequence.
ACCESSION   DASRBV010000138 DASRBV010000000
VERSION     DASRBV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565729"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFA02_05285"
     CDS             <1..975
                     /locus_tag="VFA02_05285"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZU09296.1"
                     /translation="TLGWPLTTKEYGGAWIYGISDTQISIGYVTGLDYEDPRTDPHHV
                     FQSFKEHPFTRRLLEGGKMLRYGAKSLPYGGWLTIPRIYGNGWMLLGDSASFLNSQRL
                     KGIHLAIKTGMLAAETAFDALLSGDSSADTLSEYKASVDASWIRDELYPVRNFHQAFE
                     HGRTEGIIRAGIQYVLRGSNLGPDYQNEAGHLRMRPLDELGNWPGERAPFLYNAKGDG
                     KLTFDRLTDLYHSGTRHDDDQPTHLVVADLNICNTRCTKEYGSPCQYFCPAAVYEMVE
                     SSESPSGKELHINFANCVHCKTCDIMDPYQIINWVPPEGGGGPNYDGM"
     gene            1119..1658
                     /locus_tag="VFA02_05290"
     CDS             1119..1658
                     /locus_tag="VFA02_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU09297.1"
                     /translation="MPFIHIRVATNNDVPALKDLIERSVRELQSGWYTPQQIEAAVHS
                     VFGVDTQLIADGTYYIAEIEGRIAGCGGWSRRGTLYGASHFDHSRNDVLLDPARDPAR
                     IRAFFTHPEFARRGIGRALLKHCENAAREAGFRSMEMAATLPGVPLYEANGYKTLETF
                     DVPLPDGQTIQVKRMMKPI"
     gene            1724..2287
                     /locus_tag="VFA02_05295"
     CDS             1724..2287
                     /locus_tag="VFA02_05295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZU09298.1"
                     /translation="MVFVLDNYDSFTYNLVQYMGELGAEMLVRRNDELTPAEVEALHP
                     ERILISPGPCTPQDAGISIELVRHFAGKVPILGVCLGHQAIGAAFGGRVIRAPQLMHG
                     KTSQVTHDGKTIFKDLSSPMTCTRYHSLIVSEKGLPADLEISARTADGTIMGLRHRRY
                     PVEGVQFHPESVLTQEGKQLIRNFLEL"
     gene            2291..3226
                     /locus_tag="VFA02_05300"
     CDS             2291..3226
                     /locus_tag="VFA02_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HZU09299.1"
                     /translation="MWLKYPLLKGRLKYLLLFGTAVAAGAQQKPIAYVPTDGVTVSGP
                     LEVANGKATIGNNGSITAGDKTARITLARGGELRLCASTQVHITADQSIAPDSSALMF
                     ALDRGAIEASYQPGKYSDVLMTPDLRILISGPGDADLKVRVNKQGDTCIDNHGDNAPY
                     VTVTSQFEDGVYRVKPNQRVLFEHGSLDQVVDNEQEPCGCPPEKPVEMANTNGKPVGG
                     PSSTPADTAFPLAVSEGLKPPPSPPTQPVVPPGTPHAQVDVPLTYNGQAADMPSAEAP
                     PVPAAAPRQTQAKGASSRGFFHKVGHFFAHIFGRG"
     gene            complement(3242..5272)
                     /locus_tag="VFA02_05305"
     CDS             complement(3242..5272)
                     /locus_tag="VFA02_05305"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZU09300.1"
                     /translation="MVLLTLLYACVVFLSAFLLFLVQPMVAKQLLPVLGGSAAVWTTC
                     LVFFQSALLLGYLYADRVATRLRPRTQAIVHVLLLTAALATLGLRVHPNAWAATWHPF
                     ITVFVLLTLIAGLPYFALSATTPLLQAWYSGSFQAPMPYRLFSLSNAGALLALVIYPV
                     LVEPYFALKTQTRCWSAGFFLFAVLCGAIAWSVRHTLPRREAAIHAQKIPTGTLALWM
                     LLPCCSSLLLCAFTTHLGQNIAAIPLLWILPLTAYLLSFILAFHSPRTYPRWLMLRLT
                     ACTLGVLGYVAYGTQTTLPVQVLVPLYCISLFVICFFLHGELYRLRPAAEEATKFYLA
                     LSFGSALGALFAGVAAPILFRANYELVGGLVLAMVLALATTWSFGFMARLFWATGILA
                     LAWIGFVQAKAWRQDTLVQLRSFYGTLRVTQTHWPPKAKVTRTLYHGTIQHGTQMFGN
                     GMRMQPASYYGPGSGVGLAMRLCCVGRLKHVGVVGLGTGTLAVYGRPGDTFRFYEIDP
                     LVERLARELFTYLRETPAQADIILGDARYSLDRDRTGPFDVLVLDAFSGDAIPVHLLT
                     AQALTLYRKHLTPDGILAFHISSQYLNLEPVLAREAQQAGLHAITVRSAGNESLGIFA
                     AEWVLLTRNEQFLSQPEVVNASNPSLLQANVRLWTDDYASLLPVLHWQPRTH"
     gene            complement(5349..5750)
                     /locus_tag="VFA02_05310"
     CDS             complement(5349..5750)
                     /locus_tag="VFA02_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU09301.1"
                     /translation="MAVNTRFATGVQALLLLAAGPEVLHTSDDVAKALNTNPVVVRRV
                     FLQLQEAGLVKSIKGPSGGTRLARPAKEITLADVYRALDGGDIFHANQTGKGSSALAQ
                     VFRGAQKALEKELAQTTLNQLAKKVAKKEKH"
     gene            5883..6680
                     /locus_tag="VFA02_05315"
     CDS             5883..6680
                     /locus_tag="VFA02_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZU09302.1"
                     /translation="MRILFVGDIFGSAGRRIVREHIGHVIEEREIDLVIINGENAAGG
                     FGLTPAIADELFDLGAHVITTGNHVWDKREIIEYLQSVPADSHERPRRVIRPANYVAG
                     TPGHGFYQGQLPSGQPYAVINLQGRVFMGQSDDPFRTADALLQKITAKVIFVDFHAEA
                     TSEKAALGWYLDGRVTAVIGTHTHVPTADDRILPGGTAYQTDVGMSGPYDSIIGVEKE
                     LVLQRFLTGMPGKFEAAKGDPRMAATIIECDGETGKARSIQRLLLGE"
     gene            complement(6847..8700)
                     /locus_tag="VFA02_05320"
     CDS             complement(6847..8700)
                     /locus_tag="VFA02_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU09303.1"
                     /translation="MRAERSGRYVGSARTAADASKKKTDLKKVLPEIWKLIRPRRWLL
                     FFGLFLMAINRVAGLVLPASTKFFIDTVLRQHRNDKLLPLVGVVFAATAIQAVTSFSL
                     TQLLSKAAQRMIADLRKQVQRHIGLLPVSFYDSNRTGTLVSRIMTDVEGVRNLIGTGL
                     VEFLGGIMTAVLAFAVLMHTSRKITLIVFAVIAAFVLILQKAFKTIRPIFRERGRINA
                     EVTGRLTESLGGVRVVKGYYAEAREAGVFAQGVQRLLDNIMRSLTATSALSFAATTVL
                     GIVGGLVMLLGGRDYFAHRLTLGDYFQYNMFLAFMIAPVFQIVNIGTQLTEAVAGLDR
                     TIEIMSEMDEFSDPQRTVTIGEIRGDVEFQDVRFSYEPDKPVLHGISFRSQPGTVTAL
                     VGSSGSGKSTIISLVCGFHKPDSGRVLVDGIDLATVRLDSYRSQLGVVLQESFLFDGT
                     IRENVMFSCPEATEEQLLEACRIARVDEFAERFPDGYDTIVGERGVKLSGGQRQRLSI
                     ARAILADPRILILDEATSSLDSESEQMIQHGLSYLMQGRTTFVIAHRLSTIRRADQIL
                     VVESGAIVERGTHESLYALGGRYYDLYTRQHGLETNLFLAPGEGDTVEERA"
     gene            complement(8734..9108)
                     /locus_tag="VFA02_05325"
     CDS             complement(8734..9108)
                     /locus_tag="VFA02_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09304.1"
                     /translation="MARTTILFGVILILLGIFAFVATGSHAPTALIPAYVGIVLGILG
                     FLSITEDAGRRKLYMHIAVTIGLLGFLGTAKSIYDYIRLVQGRQYPHPAAIEAKAAMA
                     AVLLVFVILCVRSFIAARRARA"
     gene            complement(9202..9705)
                     /locus_tag="VFA02_05330"
     CDS             complement(9202..9705)
                     /locus_tag="VFA02_05330"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZU09305.1"
                     /translation="MIVIVMGVSGSGKTTTALALQKLTGWQFAEGDDYHSEANRQKMA
                     SGIPLNDEDRAPWLAALHNLIHGWHERGENGILTCSALKQAYRDMLVTGLPQDAYCFA
                     LMEAPKGVIAERMRARQHFMPPALLDSQLATLQPPKEALHVSALQSPEEAAREILDAL
                     GIVPAAQ"
     gene            complement(9702..10637)
                     /locus_tag="VFA02_05335"
     CDS             complement(9702..10637)
                     /locus_tag="VFA02_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZU09306.1"
                     /translation="MKSVNWAVAHYFSSLSCAVLFACLLLAGGCRKTAPQTAQSGSDA
                     GANHYAVRGKIITVDAQHGEIVLDAAAIPGYMEAMTMPYKLKNPSIISELHPGDSITA
                     TLIATDSSDLLDQIVIVAQAKPDYKPSFYYHPLQPGDKVPDFKLLNQEGKQIHLAQFH
                     GKVLLMTFIYTRCPLADYCPRMSRNFAEIDKALAKDPELYKKTHLLSVSFDPDYDTPK
                     VLRSYGSAYTGNYTKETFAHWDFAAPGKNDLPAVLRYFDVGATPEKDRTITHSLSTLV
                     IGPDGKVFKWYPTNDWTPDQILNDVKQLVAEQGKA"
     gene            10638..>12364
                     /locus_tag="VFA02_05340"
     CDS             10638..>12364
                     /locus_tag="VFA02_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09307.1"
                     /translation="MLPLFDSPTPQTLAEKVCNALRDGAIVVAANARAARFLRLIYAE
                     QQRANGLEMWATPQIHDWETWAANLWRELSFMQRELPLLLTSLQEHSLWKQVQQGDAR
                     LVVSPDGMASLAEEAYALLSAYRAHDARRSPWAEQDAERFRQWAADFERLCRSHNWTS
                     RSHLEDELATAVKAGALPLEKNILLVGFYRLTPQQQRFIAVLREAGAVVETFEPELSE
                     SCAGPIVVADDLRDELTTCAQWCRNLLEKKGARRIGVIVPDVRSVRAEADRIFRAVLM
                     PESLDIAKDPRPTAYEFSLGMPLSEVPVVRAALLMLRWTTTPLKEEEVTWLMLSGFFC
                     KYAAEVRKLAWADFELRGSGTLSPEISLENFAKKIKPCAFRTRVLHVQRVLEKHRGAR
                     PSAAYAHWCEITERLLRTSRWPGHRQPDRVQYQAQTRWQRLLDEITLLDFSGRSVPFK
                     EFVNGLEQRATETIFTPESHHAPVQIMGAFESSGQTFDAVWFLGADDSQWPAAGRPHP
                     LLPQALQREAGMPHSSAVIDTGLAHTVTQRIANSAPEFVFSYARQNKEGEQRPSPLLA
                     RIFGTNYKTL"
BASE COUNT         2803 a         3478 c         3592 g         2491 t
ORIGIN      
        1 acattgggct ggccgctgac cacaaaggaa tacggaggcg catggattta cggcatcagc
       61 gacacgcaga tttctatcgg ctacgtcacc ggactcgact acgaagaccc gcgtactgat
      121 ccgcaccatg tcttccagtc cttcaaggag catccgttca cgcggcgtct gctcgaaggc
      181 ggaaagatgc ttcgctatgg cgcgaagtct ctgccgtatg gcggatggct gaccattccg
      241 cgcatctacg gcaatggatg gatgctgctg ggtgactcag ccagttttct caattcgcag
      301 cggctcaagg gcattcatct tgctatcaag acgggaatgc tcgccgctga gacggccttc
      361 gacgcgctgc tttccggcga cagctccgcc gacacactga gcgagtacaa agcatcggta
      421 gatgcaagct ggatacgcga tgaactttat ccagtgcgca attttcatca ggcgttcgag
      481 catggacgaa cggagggaat catccgggcg ggcatccaat atgtgctgcg cggcagcaat
      541 ctggggccgg actaccagaa cgaagcagga catctgcgca tgaggccgct tgacgagctt
      601 ggcaactggc cgggcgagcg ggcgccgttc ctctacaacg cgaagggtga cggtaagctg
      661 acttttgacc gtctcaccga cctctaccac tctggcacgc gccatgatga cgaccagccc
      721 acgcatctgg ttgtggccga cctgaacatc tgcaatacgc gctgcacaaa agagtacggc
      781 agcccgtgcc agtacttctg ccccgcggcg gtgtatgaga tggttgagtc cagtgaatcg
      841 ccctcgggca aggagctgca catcaacttt gccaactgcg tgcattgcaa gacgtgcgac
      901 atcatggacc cgtatcagat tatcaactgg gtgcctccgg agggcggcgg cgggcccaat
      961 tacgacggga tgtgagtcat ggccgcacgg gcggacgccg cttcgcggcc atttactact
     1021 gaatgaacga actgcatgtc cttcgtcgct ttgctcctca ggatgacagc ttttacttac
     1081 tgaaagatcc ttttcgttat atcgcttaac aagaccctat gcctttcatc cacattcgcg
     1141 tcgcaacaaa caacgatgtg cctgcgctca aagacctcat cgagcgctcg gtgcgcgaat
     1201 tgcagtccgg atggtatacg ccgcagcaaa tcgaggcagc ggtgcattcg gtcttcggag
     1261 tggacacgca gcttatcgct gacggcactt actatattgc tgaaattgag ggcagaatcg
     1321 caggctgcgg aggatggagc cggcgcggca ccctctatgg cgcaagccac tttgaccatt
     1381 cccgcaacga tgttctgctc gacccggcac gcgatcctgc gcgcatccgc gccttcttca
     1441 cgcatcctga atttgcgcgc agaggcatcg gcagggccct gttgaagcat tgcgaaaatg
     1501 cggcgcgcga agctggattc aggagcatgg agatggcagc caccctgccc ggcgttccgc
     1561 tttatgaggc gaatggatat aaaactctgg aaacattcga tgtccctctg cctgacgggc
     1621 agaccatcca ggtgaaacgg atgatgaagc cgatttagtg atctgtttct gaatcgtttt
     1681 gagaacagtt cgcctactca gatagactgg aagacaagcc ggtatggttt ttgttctcga
     1741 caattacgac tccttcacct acaacctcgt gcaatacatg ggtgagttgg gggcggagat
     1801 gttggtccgc cgcaatgacg agttgacgcc agccgaggta gaggccctgc atcctgagcg
     1861 cattctgatt tcgcccggac cctgcacgcc gcaggatgca ggaatttcca tcgagttggt
     1921 tcgtcacttt gcgggcaagg tcccgatttt aggcgtatgc ctcggacacc aggccatcgg
     1981 cgcggccttt ggcggaagag tcatccgcgc gccccagttg atgcacggca aaaccagcca
     2041 ggtcacgcat gatggcaaga ccatcttcaa agacctgagc agtccgatga cctgcacccg
     2101 ctatcactcg ctgatcgtct ctgaaaaagg actccctgcc gatcttgaaa tctcagcgcg
     2161 caccgcagat ggcaccatca tgggcctgcg gcaccgcagg tatccggtcg agggcgtgca
     2221 gtttcatcct gagagcgtgc tcacacaaga gggcaagcag ctcatccgga attttctgga
     2281 gctctaggct gtgtggctga agtatccact tttgaaaggc cggttgaagt atctgcttct
     2341 gttcggcacg gccgtggccg caggtgcaca gcagaagccc atcgcttatg tgcctacgga
     2401 tggagtgacc gtctccggcc cgcttgaagt agcaaacggc aaagcgacca ttggaaacaa
     2461 cggtagcatc actgccggcg acaaaaccgc aaggattact ctggcgcggg gcggcgagct
     2521 tcgcctgtgc gcctctacac aggtccacat caccgccgat cagtccattg ctcccgactc
     2581 ttctgcgctg atgtttgctc tcgatcgcgg cgcgattgag gccagctatc agccgggaaa
     2641 atactccgac gtgctgatga cgccggacct ccgcatcctc atctccgggc cgggggatgc
     2701 cgacctgaag gtgcgcgtca acaaacaggg cgacacctgc attgacaacc acggggacaa
     2761 cgcgccgtat gttacggtca ccagccagtt tgaagacgga gtttaccggg taaaaccgaa
     2821 ccagcgggtg ctctttgagc atggcagcct tgaccaggta gtagacaatg agcaggaacc
     2881 atgcggctgt cccccggaga agcctgtaga gatggccaac acgaatggca agcccgtcgg
     2941 cgggccgtct tcgacgccag cggacacagc ttttcctctc gctgtaagtg aagggctgaa
     3001 gccgcctcct tcgccaccca cacagcctgt tgtgcctccc ggaacacctc acgcgcaagt
     3061 agatgtgcca ctcacttaca acggacaggc agcagacatg ccttcagccg aagctccacc
     3121 ggtccccgcg gcagctccgc ggcagacgca ggcaaagggc gcgtcttcac gcggattctt
     3181 ccataaagtt ggccacttct tcgcgcatat ctttggccgc gggtaagaag cactgccacc
     3241 atcaatgcgt gcgcggctgc cagtgcagga caggcagcag gctggcgtaa tcgtcggtcc
     3301 acaaacgaac gtttgcctgc agcagcgaag ggttggacgc gttgactact tccggctgcg
     3361 acagaaactg ttcgttcctg gtaagcagca cccattctgc tgcaaagatt cccagggatt
     3421 cgttccccgc tgaccggact gtaatggcat gaaggccggc ctgttgcgct tcgcgcgcca
     3481 gcaccggctc cagattcagg tattggctgg agatgtggaa cgcgagaatg ccgtccggtg
     3541 tgagatgttt tcgatataag gtaagcgcct gcgcggtaag cagatgcacg gggatggcat
     3601 ctccggaaaa cgcatccagc acaagcacat cgaaaggccc ggtgcggtcg cggtccagcg
     3661 aatagcgtgc atcgccaaga atgatgtctg cctgcgcggg cgtttccctc agataggtga
     3721 acaattcgcg ggcaaggcgc tccaccagag gatcaatttc gtaaaaccgg aaggtgtctc
     3781 cgggccgtcc gtagaccgcc agagtgcccg tgcccaagcc aacaacacca acgtgtttca
     3841 accgtcccac gcagcaaaga cgcatggcaa gtccgactcc cgaacctggg ccatagtagg
     3901 aggcgggttg cattctcatg ccattcccaa acatctgtgt gccgtgttgg atggtcccgt
     3961 gatagagcgt gcgcgtgact ttggcctttg gcggccagtg cgtctgggtc acgcgcagag
     4021 tcccatagaa gctgcgcaac tggacgagcg tgtcctgccg ccacgctttg gcctgcacga
     4081 accctatcca tgcgagagca aggattcccg tggcccagaa gagacgcgcc ataaaaccga
     4141 agctccaggt agtggccagg gcaaggacca tggccagcac caggccgcct accaattcat
     4201 agttcgcgcg gaagagaatg ggagcggcca ctccggcaaa tagtgctccc aaggctgagc
     4261 caaatgagag cgcaagataa aactttgtag cttcttcagc agcgggccgc agcctgtaca
     4321 actcgccgtg cagaaagaag caaatcacaa acaacgagat gcagtagaga ggcaccagta
     4381 cctgcacggg cagtgttgtc tgcgttccgt aggcaacata tcccaaaacg cccagcgtgc
     4441 aggccgtaag ccgcagcata agccatcgcg gataggtccg cggcgaatga aaagcaagga
     4501 tgaaactcag cagataagcg gtaagtggca atatccagag cagcggaatc gcagcaatgt
     4561 tctggcccag gtgggtagta aaagcgcaaa gcaacagcga cgagcagcaa ggcagaagca
     4621 tccagagggc gagcgtcccg gtgggtattt tttgcgcatg tatggccgct tcacgcctgg
     4681 gcaaagtatg gcggacactc caggcgattg ctccacagag cacggcaaac aggaagaacc
     4741 cggcagacca gcagcgggtc tgggtcttca gcgcgaagta tggttccaca aggacgggat
     4801 aaattaccag cgccagcaac gcgcccgcat ttgagaggga aaacagccgg tacggcattg
     4861 gggcttgaaa acttccggaa taccacgcct gcagcagcgg agtcgttgcg gaaagcgcaa
     4921 aatacggcag gccggcaatc agcgttaaca acacgaatac agtgataaat ggatgccatg
     4981 tggcagccca cgcattcgga tgaacacgca gtcccagcgt tgccagcgcc gcagtcaaca
     5041 gcagcacatg cacgatggcc tgtgtacgcg gacgcaggcg cgtggccact ctgtccgcat
     5101 acagataacc gagcagcagc gcggattgaa aaaaaacgag gcatgttgtc cagacagcgg
     5161 ctgatccgcc aagcacaggc agaagctgct tcgccaccat gggctgaacc agaaaaagta
     5221 aaaatgcact caggaaaaca acacaggcgt agagaagtgt gagcaggacc atgagtgtgc
     5281 ggctggaacg gtatccagcc tacgatgaag atgcccttct ggtctacttt cggacctgag
     5341 agcaggcctt aatgcttttc ctttttcgcc accttctttg cgagctggtt caaagtagtc
     5401 tgcgccagtt ccttttccag tgccttttgc gcgcctctaa agacctgcgc cagggcggaa
     5461 cttcccttgc cagtctggtt cgcatggaag atgtcgccgc catccagagc acgatagaca
     5521 tcagcaagcg taatttcctt tgccgggcgc gccagccgtg tgcctccgct tgggcctttt
     5581 atgcttttca caagaccggc ttcctgcaat tgcaaaaaga cacggcggac gacgaccgga
     5641 tttgtgttca gtgccttcgc cacgtcatct gacgtatgca gtacttccgg tccagcagcg
     5701 agcaaaagca atgcctgtac tccggttgcg aatcgggtat tgacggccat aaagccacgc
     5761 taccatagct ttgcgccgaa ttcgtatcag aaatattaca aaccccgctc gtgcgacaat
     5821 gaaaaattac gctgccatga cgcggcatca taaattctta tgcagtcgaa aggttcctct
     5881 ttttgcgcat tctgttcgta ggagacatat tcgggagcgc gggacgccgc atcgtgcgcg
     5941 agcacatcgg ccacgtcatc gaagagcgtg agattgacct cgtgatcatc aacggcgaaa
     6001 acgccgcagg cggcttcggg cttactccag ccattgccga tgagttgttc gatttaggcg
     6061 cgcatgtaat taccaccgga aaccatgtct gggacaagcg cgagattatc gagtatcttc
     6121 agtctgttcc tgctgacagt catgagcggc cgcggcgcgt cattcgtcct gcgaactatg
     6181 tagcgggcac gcccgggcac gggttttatc aggggcagct tccctcaggg cagccatatg
     6241 ccgtcatcaa tcttcagggc cgtgtcttta tgggacagag tgacgatccg ttccgcacgg
     6301 cggatgccct cctgcaaaag atcacggcca aggtcatttt tgtagacttc catgccgagg
     6361 caacttcaga aaaagcagca ttgggatggt atctggatgg ccgcgtcacg gccgtcatcg
     6421 gcacccatac gcacgttccc acagcagacg accgtattct tcccggaggc acagcttacc
     6481 agaccgatgt cggcatgagc ggcccctacg acagcattat cggcgtggaa aaagagctgg
     6541 tgctccagcg ttttctgacc ggaatgccag gaaagttcga ggccgccaaa ggcgacccgc
     6601 gcatggcagc cacaatcatt gaatgtgatg gagaaaccgg caaggcgcga agcatccagc
     6661 gcctgctgct cggtgagtag gattccgccc gggtctggag gtctccctac tttttggata
     6721 aaagtcctga aacggaagga gggggaaatc ttagcagaat tggctaaatc cttcatcgct
     6781 aagtttcttc ccccttcggt cgtcagaatg acggattcta cgtggatgaa aaatgctgag
     6841 gaatgatcag gccctctctt ccacagtatc tccctcaccc ggagcgagga acaggttcgt
     6901 ttccagtcca tgctggcgcg tgtagagatc ataataacgg cctccgagcg catacaggct
     6961 ttcatgagtg ccgcgttcta cgatggcccc agactcgaca accagtatct gatcggcgcg
     7021 gcgaatggtg gacaaccggt gggcgatgac aaacgtagta cgaccctgca tcaggtagct
     7081 gaggccatgc tggatcatct gctcggattc ggaatcgagc gaagaagtgg cctcatccaa
     7141 aatcagaatg cgcgggtcgg caaggatggc gcgcgcaatg gagagtcttt ggcgctggcc
     7201 gccggaaagc ttgacaccgc gttcaccgac gatcgtgtcg tacccgtcag ggaaacgttc
     7261 ggcgaattca tctacgcggg caatgcgaca ggcctccaga agctgctctt ctgttgcctc
     7321 aggacatgag aacatcacgt tctcgcggat ggtgccgtcg aaaagaaacg actcctgcag
     7381 gacgacgccg agctggctgc ggtagctgtc gagccggacg gtggcaagat ctatcccatc
     7441 caccagcaca cgacctgaat ccggcttatg gaagccgcag accaggctga tgatggtaga
     7501 tttgcctgac cctgaggagc cgaccagcgc cgtgactgtt cccggctgtg agcgaaaaga
     7561 aatgccatgc aggaccggtt tgtccggctc atagctgaag cggacatctt gaaactcaac
     7621 gtcgccgcga atctcgccga tggtcactgt cctctgcggg tcgctgaatt cgtccatctc
     7681 gctcatgatc tcaatggtgc ggtcgagtcc ggcgacggcc tcggtcaact gtgtgccgat
     7741 gttcacaatc tggaataccg gagcaatcat gaaggcgaga aacatattgt attgaaagta
     7801 gtcgccgagc gtaaggcgat gggcgaagta atcgcgtccc cccagaagca tgaccaggcc
     7861 gcccacaatg cccagaacag ttgtggctgc aaatgacagt gcgctggtgg cagtcagaga
     7921 gcgcatgatg ttatcgagaa ggcgctgcac accctgggca aagaccccgg cctcacgagc
     7981 ttcggcgtaa tagcccttta cgacacgcac tccgccgaga gattcggtca ggcggcctgt
     8041 gacttcggcg ttgattcttc cgcgctcccg aaaaatgggc cggatggtct tgaatgcctt
     8101 ttgcagaatg agcacgaagg cggcaatgac ggcaaacaca atcagcgtaa tcttgcggct
     8161 ggtgtgcatc agaacggcga aggccaggac ggcggtcatg attccgccca gaaactcaac
     8221 caggcctgtg ccgatgagat tacggacgcc ttctacatcc gtcatgatgc gcgaaaccag
     8281 agtgcctgtg cgattgctgt cataaaagct gaccggaagc agaccaatgt gccgctgcac
     8341 ttgtttgcgc agatcggcaa tcatgcgctg tgccgccttg gaaaggagct gcgtgagcga
     8401 gaacgaagta acggcctgaa tagccgtagc cgcaaagaca actccgacca aaggaagcag
     8461 cttgtcgttg cgatgttgcc gcaaaactgt atcaatgaaa aatttggtgg acgcgggcag
     8521 gacgagtccg gcaacgcggt tgatggccat cagaaacagg ccaaagaaca agagccagcg
     8581 gcgtgggcga atgagcttcc atatttcagg aagcactttt tttaagtcgg tcttcttttt
     8641 cgaagcgtcc gcagcagtgc gggcagagcc aacgtaccta ccgcttcgtt ccgcacgcat
     8701 cgttgtcatg gagccgggcc gcatacaaaa tacctaagcc cgcgcccgcc gtgccgcgat
     8761 aaacgaacga acgcagagaa tgacaaagac aagcaggaca gcagccatgg ccgctttggc
     8821 ttcaatcgcc gcgggatgcg gatattgccg gccctgcacc aggcgaatgt agtcatagat
     8881 gcttttcgcc gtgccgagga aacccagcag tccaatggtg accgcgatat gcatatagag
     8941 ctttcgcctg ccggcatcct cggtaattga aagaaaaccc aggatgccca gcacgatgcc
     9001 tacataggcg ggaatcagcg ctgtcggcgc atgactaccc gtagcaacaa aggcaaaaat
     9061 gccgaggagg atcaaaatta caccgaaaag aatggtcgtt cttgccatgc ctggaagggc
     9121 ctccattgat tggaaaaagt gtatatgtta agcaatctga cctatcttca taagactcgg
     9181 aagatgaagc ggggactcaa tttattgtgc ggcgggaaca attcccagcg catctaaaat
     9241 ttctctggct gcttcttcgg gcgactgaag cgccgaaaca tgcagggcct ctttgggagg
     9301 ctgaagggtg gcaagctggc tgtcgagcag cgcaggaggc ataaagtgct gccgcgcccg
     9361 catgcgttcg gcgataacgc cctttggggc ctccatgaga gcaaagcagt acgcatcctg
     9421 cggaaggcca gtcacgagca tgtcccggta tgcctgcttg agcgccgaac aggttaggat
     9481 gccgttttcg ccgcgctcgt gccagccgtg aatcagatta tgcagggcag ccagccacgg
     9541 ggcgcggtcc tcatcattga gcgggattcc ggatgccatc ttctggcggt tggcttcaga
     9601 atgataatca tctccctcgg cgaactgcca tccggtaagt ttttgcaggg ccagagcggt
     9661 cgtggttttc cctgagccgc tgacccccat cacaatcacg atcatgcttt tccctgctcc
     9721 gcgaccaact gtttcacgtc gttaagaatc tgatctggcg tccagtcgtt tgtgggatac
     9781 cacttaaaca ccttgccgtc cggcccgatc accagcgtcg aaagcgaatg cgtaatggtg
     9841 cggtcctttt ccggcgttgc gcctacgtca aaatagcgca ggaccgcagg caggtcgttc
     9901 ttgccgggcg cggcgaagtc ccagtgcgca aaggtctcct tcgtgtagtt cccggtatag
     9961 gcactgccgt agctgcgcag caccttcggg gtgtcgtaat caggatcaaa gctcacactc
    10021 agcaggtgcg tcttcttgta aagctcgggg tctttggcaa gggccttgtc tatctcagca
    10081 aaattgcggc tcatgcgcgg gcagtaatcg gccagggggc agcgcgtata gatgaacgtc
    10141 atcagcagga ctttgccatg aaactgcgcc aggtggattt gctttccttc ctggttcagg
    10201 agcttgaagt cagggacttt gtctcccggc tgaagggggt ggtaatagaa actcggctta
    10261 tagtcgggtt tggcctgcgc gacgatgacg atctggtcca gcaggtcgga gctgtcggtc
    10321 gcaatcagtg ttgccgtgat gctgtccccc ggatgtaatt cgctgatgat actggggttc
    10381 ttcagcttat atggcatcgt catcgcttcc atatagccgg ggatggccgc cgcgtccagg
    10441 acgatttccc catgttgcgc gtccacagtg atgattttgc ctcgcactgc gtaatggttt
    10501 gccccggcat cggaaccaga ctgcgcggtt tgcggagcgg tcttgcggca tccaccagca
    10561 agcagaagac aggcaaaaag aacagcgcag gacaggctgg aaaaatagtg ggccacagcc
    10621 cagtttacac ttttcatgtg cttccactgt tcgattcgcc cacgccgcag acactggcgg
    10681 aaaaggtctg caatgcactg cgcgatggcg ctatagttgt cgctgccaat gcccgcgcag
    10741 cccggtttct gcggctgatt tatgccgagc agcagcgcgc caacgggctg gagatgtggg
    10801 cgaccccgca gatccatgat tgggagacgt gggcggcgaa tctgtggcgc gaacttagtt
    10861 ttatgcagcg ggaacttcca ctgcttctga cctcgcttca ggaacattct ttatggaaac
    10921 aggtgcagca aggtgatgcg cgccttgtgg tttcgcctga cggaatggcc tcgctggcag
    10981 aagaggcata tgcgttgctc agcgcatacc gcgcgcacga tgcgcggcga tcaccgtggg
    11041 ctgagcagga tgccgagcgc ttccgccaat gggccgctga ttttgaaagg ctctgccgca
    11101 gccacaattg gaccagccgc agccatctgg aagatgagct tgccactgcg gtaaaggctg
    11161 gggccttacc actggagaaa aatattctgc tggtgggctt ttaccgcctg acgccacaac
    11221 agcagcgttt tatagcggtc ctgcgcgaag ccggggctgt tgtagaaacc ttcgaaccgg
    11281 agctatctga aagttgcgct ggccccatcg tagttgcgga tgatctgcgc gatgagctta
    11341 cgacgtgtgc gcagtggtgc cggaatctgc tcgaaaaaaa aggggcccgg cgcattggtg
    11401 taattgttcc ggatgtccgc agtgttcgcg ccgaagctga ccgcatcttc cgggcagtgc
    11461 tgatgccgga atcgctcgat atcgccaaag acccgaggcc gacagcttat gaattttctc
    11521 tgggcatgcc gctgtcggag gtccctgtgg tccgagccgc gctgctgatg ctgcgctgga
    11581 caaccactcc gctcaaggaa gaagaagtaa cgtggctgat gctgtcaggc ttcttctgca
    11641 agtatgctgc tgaggtccgc aaactggcat gggcagactt cgagctgcgt ggttcgggaa
    11701 cgctttctcc ggagatttct ctggagaact tcgcaaaaaa aataaagcca tgcgcatttc
    11761 gcacgcgcgt gctccatgtg cagcgggtgc tggaaaagca ccgcggggcg aggccgtccg
    11821 ctgcttatgc gcattggtgc gaaattaccg aacgcctgtt gcgcacaagc cggtggccgg
    11881 gacatcgcca gccggacaga gtgcagtatc aggcgcaaac gcgctggcaa aggctgctgg
    11941 atgaaattac gctgctcgac ttctctggcc gcagcgtacc gttcaaagaa tttgtgaatg
    12001 gcctggaaca gcgcgcgaca gagaccatct ttacgcctga gtcgcatcat gctcctgtgc
    12061 agatcatggg cgcatttgaa tcatctggcc agacatttga tgctgtgtgg ttcctgggcg
    12121 cagatgactc gcaatggcct gccgcgggaa gaccgcatcc acttctgccg caagccctcc
    12181 agcgggaagc agggatgccg cacagcagcg ccgttataga caccgggctg gcacacacag
    12241 ttacacaacg catcgcaaac agcgctccgg aattcgtctt cagctacgcg cgccagaaca
    12301 aggaaggcga gcagcggcct tctccgttgc tggcccggat ttttggaaca aactacaaga
    12361 cgct
//
LOCUS       DASRBV010000139         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_361705, whole genome shotgun sequence.
ACCESSION   DASRBV010000139 DASRBV010000000
VERSION     DASRBV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361705"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFA02_05345"
     CDS             <1..469
                     /locus_tag="VFA02_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09308.1"
                     /translation="LCFAMLLAGTFVPRAHASADRVSFFHDITVGQSEEADDLVCFFC
                     SIRADGKINGDAVSFFGGVRLNATEISGDVVSFFGPVRLDGEAHIGGDCVVFGASLHR
                     SEDASIAGDTVQLPLILFLLPLLVLFLIIYGGIALARRRKYAAYPMQPPPPIR"
     gene            548..1825
                     /gene="lysA"
                     /locus_tag="VFA02_05350"
     CDS             548..1825
                     /gene="lysA"
                     /locus_tag="VFA02_05350"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZU09309.1"
                     /translation="MNSSRPFLHPDAQSPLACDGVRLDTLAKKYGTPLYVYSANQVLH
                     RLRLFTDAFQSVPHLVCYAVKANSSLAILKLLAEHGAGFDIVSGGELERVLRVSKQAT
                     GRVVFSGVGKTAEEIDLALRSGILMFNVESAGELELLAQRASKLRIHARVALRVNPDV
                     FAETHPYISTGLREHKFGIDIHEARKVYKFAARQKYLATHGVSVHIGSQIRSADPFGA
                     AAARVAQLVLELRADGLPIHSVDVGGGLGIEYHSGHSFDPEQKVRQYADALLKALRPV
                     EDLQLLLEPGRFIVAQAGALVTRVLYVKKNGRKTFVITDAAMNDLVRPALYQAHHEIV
                     PVVRRRGARKRAVDVVGPVCETGDFFARDRELPELQPGDLVAVLDAGAYGFSLSSNYN
                     SRPRPAEVLVEGKRHRLVRRRESIDDLLAPELM"
     gene            2227..3633
                     /locus_tag="VFA02_05355"
     CDS             2227..3633
                     /locus_tag="VFA02_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU09310.1"
                     /translation="MPEESRIRTSVLVVDEDAAVRSACCEIVTAKGFLSQSAENVNDA
                     LSLLKGNGIDIVLLDLKGPQGRHLDLLEQVRALYPETAVIVMTAYASISSAVEAMRAG
                     ASDYLTKPFTVDELSTVLERVAERRALDIAGRRLREKLRTEQGLSNIIGRSAEMDKIY
                     RILSKVALTTHPVLILGESGTGKELVARAIHSNGPNAGKPFVPVDCSSIVPTLIESEL
                     FGHVKGAFTGANRSKDGLLVSASGGTVFLDEIGEMPLDLQAKLLRALQEKEVRPVGAT
                     HRVPINVRVLAATNRNLAEMVEQGRFRKDLYYRLNVVNLRLPPLRQRRDDIPLLIAHF
                     LERKSREYGKTFRVSDEMLRVLLEYDWPGNVRELEHLIDRACAYCSGNLLQLGDAPTS
                     IVDYYSNMRPRTFNPGAQTDTSLIAPSVTPLVELEKQAIQQALEALRGDKLAAARLLG
                     IGKTTLYRKIKEYGLGEA"
     gene            3690..>4141
                     /locus_tag="VFA02_05360"
     CDS             3690..>4141
                     /locus_tag="VFA02_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09311.1"
                     /translation="MQTMLMITQILGAENCASVLAKQLGMEVEIAPNRRAGMAHLRRR
                     EYSIVVVDSALAEADPEGTETLWKHTGLAVPMQFNFAISGTARLVREARAVLGRREQE
                     QSIAMRAATVAVENELRDVVTGLVLHSQLALTEPEVPAALSARLRTVA"
BASE COUNT          844 a         1191 c         1166 g          940 t
ORIGIN      
        1 tttgtgtttt gcaatgctgc tcgccgggac atttgttccg cgcgcccacg cctctgctga
       61 ccgcgtttcc ttcttccatg acattactgt cggccagtcg gaagaagcag atgatctggt
      121 ctgtttcttt tgttcgattc gtgcagatgg caaaatcaat ggcgatgcgg tgtccttctt
      181 tggcggcgtt cgcctgaatg ctacggaaat cagcggcgat gtcgtttcct tttttggccc
      241 cgtcaggctg gatggcgagg cacacatcgg cggagattgc gttgtctttg gcgcgtctct
      301 tcaccgcagc gaggatgcct ccatcgccgg agataccgtg cagctccctc tcatactctt
      361 cctgctgccc cttctcgtct tgttcctgat catttatggc ggcatcgctc tggcgcgtcg
      421 ccggaaatat gcggcatatc ccatgcaacc tccgccacct atacggtaat gttgtctgca
      481 agactggacg tagacttgaa agatatttag gctcaaatta cttcctgatt tatcttcttg
      541 aggctgcttg aactcgtcac gtccctttct tcatcctgat gcgcagtcgc ccctggcctg
      601 cgacggtgtc cgcctggata ctttggcaaa gaaatacggc acaccgcttt atgtctattc
      661 agccaatcag gtccttcacc ggctgcgtct gttcactgat gccttccagt ctgtgccgca
      721 cctcgtctgt tacgcggtaa aggccaactc atcgctggcg attctgaaac tgctggcgga
      781 acatggtgca ggatttgata ttgtctccgg aggggaatta gagcgcgttc tgcgtgtcag
      841 caaacaggcc actggccgcg tggtcttttc cggagtcggc aagaccgccg aagagataga
      901 cctggcgctc cgctccggga tcctgatgtt caacgtggag agtgcgggtg aactggaact
      961 gctcgcccag cgtgccagca agctgcgtat ccacgcgcgg gttgccctga gggtcaatcc
     1021 cgatgtgttt gccgaaacgc acccatacat ttccaccggg ctgcgcgaac acaagtttgg
     1081 cattgatatt cacgaagcgc gaaaagtcta caaattcgcg gcccggcaga agtatctcgc
     1141 gacgcacggg gtcagcgtgc atattggctc acaaatccgc agcgccgacc catttggcgc
     1201 cgcagccgcg cgcgttgctc agcttgtgct ggagctgcgt gccgatggcc tccccattca
     1261 ctcagtggac gtcggcggag gcctggggat tgagtatcac tccgggcaca gttttgatcc
     1321 ggagcagaaa gtcaggcaat atgcagatgc cctgctcaag gcattgcggc ccgttgagga
     1381 cctgcaattg cttttggagc caggccgctt tattgtggcg caggccggtg ccctcgtcac
     1441 tcgcgtgctc tacgtgaaaa agaatggcag gaagaccttc gtcattaccg atgcggccat
     1501 gaatgacctg gtccgccccg ccctgtatca ggcccatcat gaaattgtcc ccgtggtccg
     1561 gcgcagaggc gcgcgcaagc gtgcagtgga tgtagtcggc cccgtgtgcg agaccggcga
     1621 cttctttgcc cgcgaccgcg aactgccgga attacaacca ggagacctgg ttgcagtgct
     1681 tgacgccggg gcctatggtt tttcgttgag ctccaattac aattcccgcc cccgtccggc
     1741 tgaagtgctg gtcgaaggca agcggcatcg cctggttcgc cgccgcgaat ccatagacga
     1801 tctcctggcc cccgaattga tgtgaaccca ttctgccagg ttcaattcgg gaattccgtt
     1861 gagctgtccc aatttgggat tttcccctaa gtacatgaaa gcactgtggt tatgattttc
     1921 tcccgtccgc cccagtccca ttctgggaaa ttctgttttc ctgctcagac ctgtgcccac
     1981 tgcataactc tttgtatttg agatggttat ctgccccgtc ccattttggc aatcctcatg
     2041 caatttatat gggcgaatcg ggtcgcacaa tcgccagttc ggagggaacc agtacccgga
     2101 gcagagcggt ctgcagtccg gaacggagtt actaatgttt tcctcggcag atgctgcgat
     2161 gcagtttctc tcgtaaaaac gacggaagga tttcatacaa tgctaaccac tacttctttt
     2221 ttacatatgc ctgaagaaag ccggatacgg acaagtgttc tcgttgtgga tgaggatgcg
     2281 gcagtgcgct ccgcctgctg cgaaattgtt acggcaaaag gatttctgtc tcagagcgcg
     2341 gagaatgtga acgacgcgct ctccctgctg aagggcaacg gcattgatat cgttctgctc
     2401 gatctcaagg gcccgcaggg ccggcatctg gatctgctgg aacaggtcag ggccttgtat
     2461 ccggaaacgg ccgtcatcgt catgactgcc tatgcaagca tatcctccgc agtcgaggcc
     2521 atgcgcgcgg gtgcatccga ttatctgacc aaacccttta ctgtagatga gctttccact
     2581 gttcttgagc gtgttgccga gcgacgggca ttggatatcg ccggccgccg cctgcgggaa
     2641 aagctgcgca ccgagcaggg actgagcaat atcatcggcc gcagcgcgga gatggacaag
     2701 atctaccgga ttttgtccaa agtcgccctg accacacatc ccgtactcat tctgggagag
     2761 agtggcacag gaaaggaact ggtggcgcgc gccatccaca gcaatgggcc aaacgcggga
     2821 aaaccgttcg ttccggtaga ttgcagttcg atcgtgccca ccctgattga aagcgagctg
     2881 ttcgggcatg taaaaggcgc attcaccggg gccaaccgct cgaaggacgg cctgctggtc
     2941 tccgcttccg gcgggacggt atttctggat gaaattggcg agatgccgct tgatcttcag
     3001 gccaaactgc tgcgcgcttt gcaggaaaaa gaggtgcgcc cggtaggcgc cacgcaccgc
     3061 gtccccatca acgtgcgtgt ccttgcagcg acaaaccgca atctggcgga gatggtcgaa
     3121 caggggcgct tccgcaaaga cttgtactac cggctgaacg tggtcaatct gcgcctgcct
     3181 cctctcaggc agcggcgcga cgatatacca ctgctgattg cgcattttct ggaacgcaaa
     3241 agccgggaat acggtaagac atttcgcgtc agcgatgaaa tgcttcgcgt gctgctggag
     3301 tacgactggc cgggaaacgt gcgcgaactg gagcatctga ttgaccgtgc ctgcgcttat
     3361 tgctcaggaa atctgctgca actcggcgac gctcccacat ctatcgtgga ctactactca
     3421 aacatgcgtc cgcgcacatt caaccctggt gcgcagacag acacctccct gattgcaccc
     3481 tccgtgaccc cgcttgtgga attggaaaag caggcgattc aacaggcact ggaagccttg
     3541 cgtggagaca agctggctgc tgccaggctt ttgggaatcg gcaaaaccac gctctatcgc
     3601 aagatcaagg aatacggcct gggtgaggca taacagccac caggtcatca tgattcacca
     3661 agaaagaccg ttctcgaagg agccagacaa tgcaaaccat gttaatgatt acccagattt
     3721 tgggcgcgga aaactgcgcc tccgtgctcg caaaacagct tggcatggaa gtagaaattg
     3781 cgcccaatcg ccgcgccggc atggcgcatc tgcgccgcag ggaatattcc attgttgtgg
     3841 tggacagcgc ccttgccgaa gccgatcccg aaggcacaga gacactctgg aaacacactg
     3901 gcctcgctgt tccgatgcag tttaatttcg ccatttccgg caccgcccgg ctcgtgcgcg
     3961 aggcgagggc cgttcttggc cgtcgcgagc aggaacagtc aattgccatg cgcgccgcaa
     4021 ccgttgcggt tgaaaatgaa ctgcgggacg tggtgaccgg gctggtcctg cattcgcagc
     4081 ttgcgctcac cgagcccgag gtcccggcag cgctgtctgc ccgcctcagg acagttgctg
     4141 a
//
LOCUS       DASRBV010000140         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_515265, whole genome shotgun sequence.
ACCESSION   DASRBV010000140 DASRBV010000000
VERSION     DASRBV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515265"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VFA02_05365"
     CDS             complement(<1..1748)
                     /locus_tag="VFA02_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZU09312.1"
                     /translation="MNLEEFQRILRQAFLVPVLALLALAGILLWQITDAQKAQRWLDQ
                     SDEVSAEIAELESLIMDQETGLRGYELTGDPGMLAPYKTAAPLITNHFASLRQMISDN
                     PAQQANLSRVSERYQAWLAFAGGVLTGPAAFRNSGLDRRGAELMAGTRSAIRQMTQTE
                     VRLRQARLENTILLERREITVLLSGTLLVGLLLGFFSLSRLHRVSTAYRQSLALLDKR
                     REKLRTILRSIGDAVIVCEADGSIEFMNPVAEALTGWPENEAAGHLLDEVFHIVNEYT
                     NEVAENPVEKVRRLNKVVGLANHTVLIARSGSRHVIDDSAAPILNQQGELVGIVLVFR
                     DVTEKRRAETALAASEKLAVAGRLAASIAHEIHNPLDSIGNLHYLLAQETDPSRRAEY
                     LELAQQELDRTLQISRSLLNLYREPNAPVQVNVAEMIGSVLLLLKRRLDNQGIKVAQH
                     SADNVAIEGFPAELRQVLTNLIINAADAAGANGKIHVRSYAAQAEEFRGTGAIIEILD
                     NGPGIPAEIGPKLFQPFFTTKGNHGTGIGLWISMGIVQKHGGAIQIGNSTDPEYPGAR
                     VWVYLPAHTMATSFSRS"
     gene            complement(1745..2128)
                     /locus_tag="VFA02_05370"
     CDS             complement(1745..2128)
                     /locus_tag="VFA02_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU09313.1"
                     /translation="METNAPFTVLCVDDEAIGLQIRKALLEHAGYHVLTALSGVEGLV
                     IFESSPVDLVLLDYLMPGMNGGEVARRMRAVRSEVPILMHTACVDLPADVREFVDDVL
                     SKGEGPEALLHRLRQVLEAALKRGM"
     gene            2465..3166
                     /locus_tag="VFA02_05375"
     CDS             2465..3166
                     /locus_tag="VFA02_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09314.1"
                     /translation="MNQTIFVLEDDADISRLVQHHLEAAGFGARVYANPSNVIADAER
                     QPPSLFLLDIMVPGGDGLDVCRRLRNHAALSTVPIIFLTARASENDRVLGLELGADDY
                     ITKPFAPRELLARVKAVLRRFERPSTPSIISFEDIVIDAGAMQLKVRGELATTTATEF
                     RLLDYLARHPGRVFSRDHLLDAVWGDARFVTPRSVDVYVRRIREKIEADPENPRYLKT
                     VRGAGYRFEIPRPVQ"
     gene            3163..>3878
                     /locus_tag="VFA02_05380"
     CDS             3163..>3878
                     /locus_tag="VFA02_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZU09315.1"
                     /translation="MTGKVFTKLLLSFILVLLAGTAIFDLSLRPILERTVREQSHGAL
                     DLLLASLLALVVAILLAALLARRMSRRFDRIVLFANRIAAGDLTARLQEEDLDELSDV
                     ARALDMTASRLEQSFRELETSRRELTALLDSMQEAVVAVNAQGQVIWSNSVMQRICSS
                     AVREGRPLVELVRDPEVLSCVEGALRERQNFTGKALSVAPGKIFEVNAAPTPEGGAVA
                     VLHDVTSVERAEKTRRDFIA"
BASE COUNT          794 a         1167 c         1092 g          825 t
ORIGIN      
        1 gaacgggaaa aagacgtggc catcgtgtgc gccggcagat agacccacac gcgggcgccg
       61 ggatattcgg gatcggtgct gttgccgatc tggatggcgc cgccatgctt ctgcacgatc
      121 cccatgctga tccaaagccc aatcccagtg ccatgattgc ccttcgtcgt gaagaagggc
      181 tgaaagagtt tcggaccaat ctcggccggg ataccggggc cgttgtccag tatttctata
      241 atggccccgg tcccgcgaaa ttcttccgcc tgggcggcgt aggagcgaac atgaattttt
      301 ccattcgctc ccgcagcatc ggcagcattg ataatcaggt tggtcaagac ctggcggagt
      361 tctgcgggaa acccttcaat ggccacatta tcagccgagt gctgggcgac tttaatgccc
      421 tggttatcga gtcttcgttt gagaagcagc agcacgctgc cgatcatctc cgcaacattt
      481 acctgtaccg gtgcattcgg ctcgcgataa aggttcaaca agctgcggct gatctgcaaa
      541 gtacggtcca gctcctgctg cgccagctcc agatattcag cgcgccgcga agggtcggtc
      601 tcctgtgcca gcagataatg caggttgccg atcgaatcca gggggttgtg aatttcatgc
      661 gcaatgctgg ccgcaaggcg tcctgccacg gcaagttttt cgctcgcggc cagagcagtt
      721 tccgcgcggc gtttttccgt aacgtcacga aagacgagaa caatgcctac aagttctccc
      781 tgctgattca gaatgggcgc agcgctgtcg tcaatcacat ggcgcgatcc actgcgggcg
      841 atcaatacag tgtggttcgc aaggccaaca accttgttga gccggcgcac tttttcaacc
      901 ggattctccg ccacttcatt cgtgtactca ttcacgatat gaaagacttc gtccaacaaa
      961 tgccccgcag cttcgttttc gggccatccc gtcagcgctt ccgccaccgg attcatgaac
     1021 tcgatggagc catccgcctc gcagacaatc accgcatcgc caatcgaacg gagaatcgtg
     1081 cgcagcttct cgcgcctttt gtccagaaga gccagcgact gccggtaagc ggtggaaacg
     1141 cggtgcaggc gggagagcga gaaaaagccc agcagaaggc caaccaacaa cgttccactc
     1201 aagagcaccg tgatctcgcg ccgctccaga agaatggtgt tttccagccg tgcctgcctg
     1261 aggcggactt ccgtctgggt catctgccgg atggcgcttc gtgtgccagc catcagctct
     1321 gcgccacgcc gatccaggcc gctgtttctg aatgcagcag gtccggtaag tacacctcca
     1381 gcaaaggcga gccacgcttg atagcgttca gagacgcgcg acaagtttgc ctgctgggca
     1441 ggattatcgg aaatcatctg gcgcagcgaa gcaaaatgat tggtaatcag cggcgccgca
     1501 gttttataag gagcaagcat gcccgggtcg ccggtcaatt cgtaaccgcg aagcccggtc
     1561 tcctggtcca taatcaggct ttccagctct gcgatttctg cgctgacctc gtcgctctgg
     1621 tcaagccagc gctgggcctt ttgcgcatcc gtaatctgcc ataaaagaat accggccagt
     1681 gccagaagcg ccaggacggg gacgagaaat gcctgcctca gaatccgctg aaattcctcg
     1741 agattcacat ccccctcttc aacgccgctt ccaggacctg acgcaggcga tgcaggagcg
     1801 cttccggccc ttcgcccttg gacaggacat catccacaaa ttcgcggaca tcagcaggca
     1861 ggtctacaca ggccgtgtgc atcaggatcg gaacttcaga acggacggcg cgcattctgc
     1921 gggccacttc gcccccgttc attcctggca ttaaatagtc aagaaggacg agatccaccg
     1981 ggctgctctc aaaaatgacc aggccttcta ctccgctcaa cgcagtcaaa acatgataac
     2041 ctgcgtgttc cagtaaggcc tttctgattt gcagtcctat ggcttcatca tcaacacaaa
     2101 gaaccgtaaa tggagcgttt gtttccatga ccgcatgggg actgaaagct tttggtttca
     2161 atgaacttgc tgccagagta tagcattaac gcattttcac aggttctgta cactccattt
     2221 gccctgcgcc gtgtcaccgg cttccagttg gtcgcggcga ctgtagtttt ggggcatggt
     2281 cctgactatc tgtgaaaatg ctatagccgc tgactggcag aagcaccggg accctggttc
     2341 ccgcccggcg ctgtggactc gcaccttcgg cgtgccctgg gtaccaaatt cacaaaacat
     2401 tagcattcct tttgcagaca tggagtaacc tgagagcggg aagctagtga ggtaagaaac
     2461 cagcttgaac cagaccatat tcgtccttga agatgatgcc gacatctcac gcttagtgca
     2521 gcatcatctg gaagcagcgg ggtttggcgc ccgtgtttat gccaacccct ccaacgtcat
     2581 tgcagacgcc gagcgtcagc caccctcgct ctttctgctc gacatcatgg tccccggcgg
     2641 agacggcctg gatgtttgcc gccgcctgcg caaccacgcg gccctcagca ccgtgccgat
     2701 tatttttctg acagcgcgcg cttctgaaaa tgaccgtgtt cttggcctgg agctgggcgc
     2761 tgacgactac attacgaagc ccttcgcccc ccgggagctg ctggcgcgcg tgaaggcagt
     2821 tctgcggcgg tttgagcggc cgtctacgcc atctattatc agctttgaag acatcgtgat
     2881 tgacgccggg gcgatgcagc tcaaagtccg cggcgaactg gccacgacca ccgccacgga
     2941 gttccgcctg cttgattatc ttgcccgcca tccgggccgc gtcttcagcc gcgaccactt
     3001 gctggacgcg gtctggggtg atgcccgatt cgtgacgccg cgctcggtgg atgtttacgt
     3061 gcggcgcatc cgtgagaaaa tcgaagctga tccggagaac ccgcgttatc tcaaaaccgt
     3121 ccgcggcgcc ggataccgtt ttgagattcc gcggcctgtg caatgacagg caaagtattc
     3181 accaaactgc tgctctcatt catcctggta ctgcttgccg ggacggccat cttcgatctg
     3241 tcccttcgcc cgatccttga gcgcacggtg cgcgaacaat cgcatggggc gcttgatctg
     3301 ctactggcct cgctgctggc gctggtggtg gcaatcctac tggccgccct tctggcccgg
     3361 cgcatgtcca ggaggtttga ccgcattgtt ctctttgcca accgtattgc cgcgggtgat
     3421 ctgaccgcgc gcctgcagga agaggacctg gacgagcttt ctgacgttgc ccgcgcgctc
     3481 gacatgactg ccagccgtct ggaacagagc tttcgggaac tggaaaccag ccgccgcgaa
     3541 ctgacagcgc tgctcgacag catgcaggag gcggtcgtcg ccgtaaatgc ccaggggcag
     3601 gtcatctggt cgaacagcgt catgcagcgg atatgctcca gcgccgtccg tgaagggcgc
     3661 cctctggtag agctggtacg tgaccctgaa gtcctctcct gcgtggaagg cgcgctgcga
     3721 gagcggcaaa actttaccgg aaaggcgctg agcgtggctc ccggaaagat ctttgaggtc
     3781 aatgccgcgc ctactccgga aggcggcgcc gttgccgtgc tgcacgatgt gaccagtgtg
     3841 gagcgcgcgg aaaagacgcg ccgcgatttc atcgccaa
//
LOCUS       DASRBV010000141         6069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_261081, whole genome shotgun sequence.
ACCESSION   DASRBV010000141 DASRBV010000000
VERSION     DASRBV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261081"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="VFA02_05385"
     CDS             complement(<1..1881)
                     /locus_tag="VFA02_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09316.1"
                     /translation="MLLLILLLCGAAEAQMFSGTVVDAQDAPVANAIVVNTYGAMLAA
                     SGRDGRFSFHLNVQGPIRIEVRALGYDTANTTLAEGMAARIVLTRQVEQVVVTAYRSP
                     LETLDSPASARVLNQQQLREAAAPALDGKLRQVPGLELFRRSSSLVANPTSQGISLRG
                     LGSTAASRTLVVSNDVPLNDPYGGWIHWEELPELAVQSVEVVRGGASDLYGSSAIGGV
                     VNVITAQPRQNALLLTTSYGAENTTDNGLLASVKRGPWAGLLAVGLLGTDGYTLVAPH
                     LRGPVDRPSNVHAQNGLVDLEREIRNRYSIFLRGSGLNEARDNGTPLQKNATRLWRYS
                     AGADVQQLSLRLYGSTEHYRQTFSSIAPGRASETITRYAEDPANELGASARWRRPIGP
                     KALFLTGADTHDVRASDDELLFTTHSLQNTTARQRQTGVYGEALATPANWTLSASARA
                     DHFSNFDAKQWTPSGPQTMPSFSETVFDPRIGISRRITTDFALHASAFRAYRAPTQNE
                     LYRTGQVGQQTTFANPNLRSERATGWETGFQADIPQWNSMLRVSYFWTQVNRPITALT
                     LQVTPTSTLLRRENLGQIESRGVSLDYEVHPAHWAALTGGYQFANATVTRYDQQPQLV
                     GNW"
     gene            complement(1919..2809)
                     /gene="gluQRS"
                     /locus_tag="VFA02_05390"
     CDS             complement(1919..2809)
                     /gene="gluQRS"
                     /locus_tag="VFA02_05390"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZU09317.1"
                     /translation="MPQTYRGRLAPSPTGLLHLGHARTFWTAYQRALAAGGTLVMRNE
                     DLDPQRSKPEFARAMFEDLRWLGIQWQEGPDVGGPYAPYEQSCRRALYLEAWRRLVEA
                     QVVYPCRCSRKDLTRSAQAPHDEDDEPVYPGTCRPQGRITAPESPAGMNWRFRVPDGV
                     EISFEDGHFGPRSYRAGRDFGDFLVWRRDDVPAYQLAVVVDDAAMRITEVVRGVDLLR
                     STARQLLLYRALRLTPPVWYHCPLVTDEQGQRLAKRHDSLSLRALREHGVQPEELISN
                     MTSAILRPGEKYEKLRYFLK"
     gene            2970..4310
                     /locus_tag="VFA02_05395"
     CDS             2970..4310
                     /locus_tag="VFA02_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09318.1"
                     /translation="MTSQRKNPYSAPWMIFWVALVVRIAYVVLAHMYRIRPYVDHFSF
                     GWEMGRIGRALATGYGFADPFHGHTGPTTWVGPAYPLIIAGVFKLFGVYTALSAFVLI
                     SINCVLNALMVRTTWEIAARCFRNAGITRWSAWIWALYPAAMQYAVKWIWEMTLSAFL
                     FSWVLVLSLRMRNIGETPEEGPGTLPFAKQRSGKQWAMFGLLWGLIALSNPSLLLFLP
                     VNGLWILAGFRKEHIVLKRQISGAVFAALIFLACIAPWTYRNWRAFHHFIPMRGNFGV
                     EFYLGNGPGATGLLMAYDHPAQDPIELEHYRQMGELAYAKWRGELAKNYIRQHPGHFA
                     DLCLRRIYFFWFSVPHPFDEGATNEYGRVVNFQFTSIVGLLGLALALKRHIPATGLFA
                     WAFLLLPLVYYVVTVHARFRHPLEPLIAILGVYLFHSAEMTWRVRWFSRSDSVV"
     gene            4307..4942
                     /locus_tag="VFA02_05400"
     CDS             4307..4942
                     /locus_tag="VFA02_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09319.1"
                     /translation="MTAYWIQRTRRHFLLAFTAAVITMIAFAATPPPDVRHRASLATA
                     YAALVFLAASMWLGPWNVLRRRANPVSFDLRRDVGIWAGLLALVHTGIGLTVHLRGRM
                     WMYFFRKLHPLSIQNTLFGFANYTGLIASLLFLMLLVLSNDVSLRWLGTRRWKSLQRW
                     IYLAFGLTVAHAIAYQMVESRRLPWVIVFAVCALAVIVVQMAGLVRKRAGS"
     gene            5039..>6069
                     /locus_tag="VFA02_05405"
     CDS             5039..>6069
                     /locus_tag="VFA02_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HZU09320.1"
                     /translation="MSLNHSRKNLSRIALAFAAFCMLAFTSLPLSAQADKDKPAQEKA
                     GAEKPSLPPLPPDAHVEQTMELNGKTLHYTVTVGTIPVYDKENKEAGRVVYTAYTVPG
                     GNRPVTFAFNGGPGASSVYLNFGAIGPKHLSVGNEGDSPSDPPTLTDNPGTWLDFTDL
                     VFIDPIGTGFSRSLVPEAETKKLFYSTIPDIEYLSRVVYDWLLKNGRMDSKKYLVGES
                     YGGFRGPRITHYLQSQLGVAMNGVVLVSPYLNPTIEDNGDVSPLPWLMTLPSITAAHL
                     EREHKLTPEVMQQVISYTLGEYATDILKGRSDPDATARIVKKVTEMTGLDPEFVKRSG
                     GRLETGAYLR"
BASE COUNT         1117 a         1737 c         1856 g         1359 t
ORIGIN      
        1 ccagttgccg acgagctgcg gctgctggtc atagcgggtc acggttgcgt ttgcaaactg
       61 atatccgcca gtgagagcgg cccagtgtgc cggatgtact tcgtagtcga gagaaacgcc
      121 gcggctttca atctggccca ggttttcgcg ccgcaacaag gtagatgtgg gcgtgacctg
      181 aagcgtgagc gcggtgatgg ggcggttgac ctgcgtccag aaatagctga cgcgcagcat
      241 ggaattccat tgcggtatgt ctgcctggaa tccggtttcc caaccagtgg cacgctcgga
      301 gcgcaggttc ggattggcga atgttgtttg ctggccgacc tgtcccgtgc ggtacagttc
      361 gttttgcgtt ggggcgcgat aggcgcgaaa tgccgaagcg tgcagggcga agtcagttgt
      421 gatgcggcgc gagattccga tgcgcggatc gaagaccgtt tcgctgaaag acggcattgt
      481 ttgtggccca gatggcgtcc attgcttggc atcgaagttt gagaagtggt ctgcgcgcgc
      541 ggacgcactc aatgtccagt ttgccggggt agcaagcgct tcgccgtaca cgcccgtctg
      601 gcgctgccgc gcagtggtgt tttgcagact atgcgttgtg aagagaagtt cgtcgtcaga
      661 agcgcggaca tcgtgcgtat ctgctccggt gagaaataat gccttgggcc cgatgggccg
      721 ccgccagcgc gcggacgcac ccagctcatt tgcggggtct tcagcataac gggtgattgt
      781 ttctgatgcg cgaccggggg cgatggacga gaaagtctgc cggtagtgct ccgtgctgcc
      841 gtagagacgc aggctgagct gctgcacgtc ggcacctgcg gagtaacgcc agaggcgcgt
      901 ggcgtttttc tgcagcggag tgccgttgtc gcgggcctca ttcagtccgc tgccgcgcaa
      961 aaagatactg taacggttac gtatttcacg ctccaggtcc accagcccat tctgcgcgtg
     1021 gacattggat ggacggtcta caggcccacg caggtgtggg gcaaccaacg tatatccatc
     1081 cgtgccgaga aggcccactg ccagcagtcc tgcccagggg ccgcgtttta cggatgcaag
     1141 cagcccgttg tctgttgtgt tttctgcgcc gtagctggtc gtgagcaaaa gcgcgttttg
     1201 tctgggctgc gcggtgatca cgttgacgac gccgccgatc gcgctcgagc cgtacaagtc
     1261 agacgcgccg ccgcgcacta cttccaccga ttgcacggcc aattcgggaa gttcctccca
     1321 atggatccat ccgccatatg gatcattgag gggcacatcg ttggaaacaa cgagcgtgcg
     1381 gcttgcagcg gttgacccaa gtccgcgcag agagatgccc tgcgatgtgg ggttggccac
     1441 cagcgagctg gaccgccgga agagttcgag cccggggacc tggcgcagtt tgccgtcaag
     1501 cgcgggggcc gcggcttcgc gtaactgctg ctggttgaga acacgcgcac tggcgggact
     1561 gtcaagcgtt tcgagtggcg agcggtaggc ggtgacaact acctgctcga cctggcgggt
     1621 gagcacgatg cgcgcagcca tgccttctgc gagcgtggta ttcgcagtgt cgtagccgag
     1681 ggcccttacc tctatccgaa ttggcccttg cacattcaga tggaaagaga agcggccatc
     1741 tctgccgctt gccgcgagca tggcgccgta cgtattaaca acgatggcgt tggcaacagg
     1801 cgcgtcctgt gcgtccacta ctgttccgga aaacatctgc gcctctgctg cgccgcacaa
     1861 cagcagaatg aggagcagca cgaatcttct ggcgcaattc tgcatttttt gtctttacct
     1921 atttaagaaa atagcgtagc ttttcgtatt tttcgcccgg gcgcaatatt gcggatgtca
     1981 tgttcgaaat aagttcctct ggctgcaccc cgtgttcccg cagtgctctg agtgagagcg
     2041 agtcgtgacg tttggcgagg cgctggccct gttcgtcggt caccagcggg cagtgatacc
     2101 agacgggagg cgtcaggcgc agggcgcggt agagcagaag ctggcgtgcg gtggagcgca
     2161 gaagatcgac gccgcggaca acctctgtga tgcgcattgc ggcgtcgtct acaacaacgg
     2221 cgagctgata ggcaggaaca tcgtcgcgcc gccagacgag gaaatcgccg aagtcgcgcc
     2281 ctgcccgata agagcgcggg ccgaagtggc catcctcaaa actgatttca acaccgtcgg
     2341 gcacgcgaaa gcgccagttc attccagcag gcgattccgg cgctgtgatt ctgccctgag
     2401 ggcggcaggt gcctggatag acaggttcgt catcttcgtc gtgcggggcc tgtgcgctgc
     2461 gggtcaggtc tttgcgcgag cagcggcagg gatagaccac ctgcgcttcc accagtctgc
     2521 gccatgcttc gaggtacagt gcgcgccggc agctctgctc atagggagcg taaggacctc
     2581 ccacgtctgg cccctcctgc cactggatgc cgagccagcg caggtcttca aacatggccc
     2641 gcgcaaattc cggcttcgag cgctgcgggt cgaggtcttc attgcgcatg accagggtcc
     2701 cccctgccgc gagggcgcgc tgatacgctg tccagaaagt gcgggcgtgg cccagatgga
     2761 gaaggccggt aggggatggc gccagacgtc cgcgatatgt ttggggcacg aggtcagttt
     2821 acggtgagtt ggagatatgt ggactggggc agacctgtcc ccggcaccca gccgggcaag
     2881 ttttatccat tcgtacgcga tcatgccgag agtgcctgcg gttgcgtccc ggatgggttc
     2941 cctgcgcttg tgtcctacaa ttccttttag tgacgtctca acggaagaac ccctattccg
     3001 cgccctggat gatcttctgg gtggcgcttg ttgtgcgtat cgcgtatgtt gtgctggcgc
     3061 atatgtatcg gatccggccg tatgttgacc atttcagctt tggctgggag atggggcgca
     3121 ttgggcgcgc tcttgctacc ggatacggtt ttgccgatcc tttccacgga cacaccggcc
     3181 cgacgacctg ggtcggcccc gcctatccgc tcatcatcgc cggggtcttc aagcttttcg
     3241 gcgtctatac ggcgctttct gcatttgtgc tgatttccat caactgcgtg ctgaatgcgc
     3301 tgatggtgcg cacaacatgg gagattgcag cgcgctgttt ccggaacgca ggtattacgc
     3361 gctggtcggc gtggatatgg gcgctctatc cggcggcaat gcagtatgcc gtcaaatgga
     3421 tatgggagat gacgctgtcc gcctttctct tttcgtgggt gctggtgctc tcgctccgta
     3481 tgcgcaacat cggcgaaacc ccggaagaag ggccgggcac cctgcctttt gcgaagcagc
     3541 ggtcaggaaa gcaatgggcg atgttcggcc ttctctgggg actgatagcg ctcagcaatc
     3601 cttcgctatt gctgttcctc ccggtgaacg gattgtggat cctcgccgga ttcagaaaag
     3661 agcacatagt gctgaagcgg cagatttccg gcgcggtctt tgcggccctt atcttcctgg
     3721 cctgcatcgc cccctggacc tatcggaact ggcgcgcctt tcaccatttc attcccatgc
     3781 gcggcaactt tggtgtggag ttctatctcg gcaacggtcc gggggccaca gggctgctga
     3841 tggcatatga ccatcccgca caggacccca tcgagctgga acattaccgg caaatgggtg
     3901 aactggctta cgccaaatgg cgcggtgagt tggcaaagaa ctacattcgc cagcacccgg
     3961 gacattttgc agacctgtgt ttgaggagaa tttacttctt ctggttcagt gtgccgcatc
     4021 ccttcgacga gggagcaacg aatgaatacg gccgggtggt gaactttcag ttcaccagta
     4081 tcgttgggtt actcggactg gccctggcgc tcaagcggca catccccgcc accggactct
     4141 ttgcctgggc ctttcttctg ctgccgctgg tctattatgt cgtgaccgtc catgcgcgct
     4201 tccgtcatcc gctcgagccg ctgattgcta tcttaggtgt atacctcttt cattcagcgg
     4261 aaatgacctg gcgcgtgcgc tggttttccc gctctgactc cgttgtatga ctgcatattg
     4321 gattcaacgc acacgcaggc atttccttct ggccttcaca gccgcggtca tcacgatgat
     4381 cgcctttgcc gcgacacctc caccagatgt acgccatcgg gcaagcctgg ccacggcgta
     4441 tgcggcactg gtgtttcttg ctgcttcgat gtggctgggg ccgtggaacg tgttgcgccg
     4501 gcgggcaaat ccggtgagct ttgatctgcg ccgcgacgtg ggcatctggg ccgggctgct
     4561 ggcgcttgtg cacaccggca tcggcctcac cgtccacctc cgtggacgca tgtggatgta
     4621 cttcttccgc aagttgcatc cgctttcgat tcagaacacg ctgtttggat ttgccaatta
     4681 cacggggctg attgcatcgc tgctcttcct gatgctgctt gtgttgtcga atgatgtttc
     4741 gctgcgctgg ctcggcacac ggagatggaa gtcgctgcaa cgatggattt atctggcctt
     4801 tggactgaca gttgctcacg caattgccta tcagatggtg gaaagccgga ggcttccatg
     4861 ggtgattgtg tttgctgttt gtgcgctggc tgtgattgtg gtgcagatgg ccgggcttgt
     4921 ccggaagcgc gccggttcgt aaggccccgg tccatctggc ttaaaaatat accgcctgaa
     4981 cacaacctgg acacaagctg gtatacagtc cgggatgcct tccgtgtatg gtatccacat
     5041 gagcctgaac cactcgcgaa agaatctcag ccgcattgct ctggccttcg cggctttttg
     5101 tatgcttgct ttcacctctc tgccgctctc tgcacaggct gacaaggaca agcctgccca
     5161 ggaaaaagca ggcgcagaaa agccttctct gccgccgctt cctcccgatg cgcacgttga
     5221 gcagacaatg gagctgaacg gcaagacgct gcactacacc gtgaccgtgg gcacgattcc
     5281 cgtctatgac aaggagaaca aggaggccgg acgggttgtc tacactgcgt acaccgtgcc
     5341 cggaggcaat cgcccggtca cgttcgcctt caatggcggc cccggcgctt cttctgtcta
     5401 tctcaacttt ggcgcgattg gaccaaaaca tctctcggtg ggaaatgaag gagacagccc
     5461 ctctgaccca cccacactca cagacaaccc gggcacatgg ctcgacttca ccgaccttgt
     5521 ttttattgat cccatcggca ccggcttcag ccgctcgctc gtgcctgaag ctgagacgaa
     5581 aaaactcttc tacagcacca taccggacat cgagtatctc tcccgtgtgg tgtatgactg
     5641 gctgctgaaa aatggccgca tggactcaaa gaagtatctt gttggcgaaa gctatggcgg
     5701 cttccgcgga ccgcgcatca cgcattatct gcaatcgcag cttggcgtag cgatgaacgg
     5761 cgtggtgctg gtctcgccct atctcaatcc gaccatcgag gacaacggag acgtctcgcc
     5821 tctgccgtgg ctgatgactt tgccctccat tacagcggcg catcttgagc gcgagcacaa
     5881 gcttacgccg gaggtcatgc agcaggtcat ctcctacacg ctgggcgaat acgcaacaga
     5941 tatcctgaag ggccgttccg accctgatgc tacggcccgt atcgtgaaga aggtcaccga
     6001 gatgaccggg cttgaccctg aatttgtaaa acgctccggc gggaggcttg aaaccggggc
     6061 ctatctacg
//
LOCUS       DASRBV010000142         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_721138, whole genome shotgun sequence.
ACCESSION   DASRBV010000142 DASRBV010000000
VERSION     DASRBV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721138"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="thiD"
                     /locus_tag="VFA02_05410"
     CDS             <1..847
                     /gene="thiD"
                     /locus_tag="VFA02_05410"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZU09321.1"
                     /translation="VMNQAAPASLERDVRPMPLALTIAGFDPSSGAGISADLKVFAAH
                     RVYGMACITALTVQSTLGVRRVEAVAPAVLRETLECLCEDALPDGVKIGMLATAENVG
                     EVVAFLASSRMAREKIVLDPVIRSSSGRELLSPEGISRVRSELLAQVGWITPNLDELA
                     VLTQQTRPLRREDVPPVAAELHRAYPHLHILVTGGHLDRPDDFLFLPSGGSFWFQGDR
                     VETTSTHGTGCALSSALLCALMSGRRPQEAVRHAKNYVTGALKAAFPTGRGRGPMHHL
                     FQCNF"
     gene            915..>2551
                     /locus_tag="VFA02_05415"
     CDS             915..>2551
                     /locus_tag="VFA02_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU09322.1"
                     /translation="MAEQEKPKQSQMQEDDVVGKAYDSRLMRRLIRYLYPYKWAALTS
                     LVAVLIKAGLDVLGPYLTKVAVDRYMTAQPPAKQSWLAHHLSANAVTGITQLATLYLG
                     ALVLSYFLEFVQTYLMQWTGQKVMFDMRSQIFRHLQYMHVGFFDRNPVGRLVTRLTSD
                     VDALNEMFTSGVFAIFEDIFVLAGIVIVMLHMSWWLALLAFAVLPFILIVTRIFRKHV
                     RDSYRRIRTAIAKINSYTQEHISGMTVVQLFNRQKRSFDQFAKVNRQHMDAFKDAILA
                     YALYYPAVELLSSIAIAIVIWRGGYGVLHATVSLGVLVAFMQYAQRFFRPIQDLSEKY
                     NILQAAMAASERIFKLLDTQPEIVAPAHPKEGDGSGRIEFRNVWFTYQKLTDEQRSRI
                     ARATSTDLAAMEDIEWILRGVSFTVEPDQTAAIVGHTGAGKTTIISLMMRFYDIQHGS
                     ILIDGVDVREHDLKQLRQRFGVVLQDPFLFTGTIAENIRLGSCWITDQRMEEAADEVN
                     VREFIHSLPGGFAEPLYERGNGLSTGQKQLINFARALA"
BASE COUNT          504 a          762 c          729 g          556 t
ORIGIN      
        1 ggtcatgaat caggctgctc ctgcatctct cgaaagagac gttcggccca tgccgcttgc
       61 cctgaccatt gccggcttcg acccctcgtc aggcgccggt atctccgcgg atttaaaggt
      121 atttgcggcc catcgggtct atggaatggc ctgcatcacg gcgctgactg tgcaatccac
      181 cctgggggtg cggcgggtgg aagctgtggc cccggcggtg cttcgcgaaa cccttgaatg
      241 tctgtgtgag gacgccctgc cggatggggt gaagatcgga atgctcgcga ccgcggaaaa
      301 tgtaggtgaa gtggtggcgt ttctggcttc gagccgaatg gcccgggaaa agattgtcct
      361 ggatccggtc atccgttcca gttcaggacg ggagctgctc tcgccggaag gcatctcccg
      421 ggtgcgttcc gaattgttgg cccaagttgg ctggatcact ccaaatctgg acgagctggc
      481 tgtgctgacg cagcagaccc gccctttgcg ccgggaagat gttcctcccg tagcagcgga
      541 gttgcaccgg gcctacccgc acctgcatat attggtaacc ggaggccatc tggatcgccc
      601 ggatgatttc ctgttcctgc catcgggagg gtccttctgg ttccagggag atagggtcga
      661 aacgacttct acccatggga ctggttgcgc cttgtcttct gccttgctct gcgcattgat
      721 gtccggcagg cggccgcaag aagccgtccg ccatgccaag aattatgtga cgggagcatt
      781 aaaggccgcc tttcccacag gcaggggaag gggccccatg caccacttgt tccaatgcaa
      841 cttctgacag catcgctgaa tcaaggccat cgagcgccgc atctgtatct cagtacaata
      901 aatacagcgc gtctatggcc gagcaggaaa agccgaaaca gtcccagatg caggaagatg
      961 acgttgtcgg caaggcgtac gacagccggc tgatgcgccg tctcatccgc tatctttatc
     1021 catataaatg ggccgcactg acgtcgctgg ttgccgtcct gatcaaagcc gggctggacg
     1081 tactcgggcc atacctgacc aaggttgccg tagaccgcta catgacggcg cagcctccgg
     1141 cgaaacagtc ctggctggcg caccatctca gcgccaatgc cgttaccgga atcacgcaac
     1201 tggccacgct ctatctgggt gcgctggtgc tgagctattt ccttgagttc gtgcagacct
     1261 acctgatgca gtggaccgga cagaaagtca tgttcgacat gcgcagccag atcttccggc
     1321 acctccagta catgcacgtc ggcttctttg accgtaatcc ggtggggcgc ctggttaccc
     1381 ggcttaccag cgacgtggat gctctgaacg agatgttcac ctccggcgtc ttcgccatct
     1441 ttgaagacat ctttgtgctc gccggaatcg tgattgtgat gctgcacatg agctggtggc
     1501 tggcgctcct ggcctttgcc gttctgcctt ttatcctgat tgtgacccgc atcttccgca
     1561 agcatgtacg cgattcctac aggcgcatcc gcaccgccat tgcgaagatc aattcctata
     1621 cgcaggaaca catcagcggc atgaccgtgg tccagctctt caaccggcag aagcgctcgt
     1681 ttgatcagtt cgcaaaggtc aaccgccagc acatggacgc tttcaaggac gccatcctgg
     1741 cctatgcgct ctactatccg gccgtagaac tgctcagctc gattgcaatt gccattgtga
     1801 tctggcgtgg tggttacgga gttctgcacg ctacggtttc cctcggagta ctggttgcct
     1861 tcatgcagta tgcgcagcgc ttcttccgcc ccatccagga cctgagcgaa aagtacaaca
     1921 ttctgcaggc ggcgatggcg gccagcgagc gcatctttaa attgttagat acgcaacctg
     1981 agattgtcgc tccggcacac cccaaagaag gcgatggctc aggacggatc gagttccgca
     2041 acgtctggtt tacctatcag aaactcacgg atgagcagcg gtcgcgcatt gcccgtgcca
     2101 ccagcactga ccttgccgcg atggaggaca tcgaatggat cctgcgcggc gtgtctttta
     2161 ccgtcgagcc ggaccagacg gcggccatcg tcggacatac cggggccggc aagaccacca
     2221 tcatcagcct gatgatgcgc ttctatgata tccagcatgg aagcattctg attgatggtg
     2281 tagacgtccg cgagcatgat ctgaagcagt tgcgccagcg cttcggcgtt gttttgcagg
     2341 acccgttcct ctttaccggg acgattgcgg agaacatccg cctgggctcg tgttggatca
     2401 cagaccagcg catggaagag gccgccgacg aggtcaacgt ccgcgaattc atccactcgc
     2461 tcccgggcgg ctttgccgaa ccactgtatg aacgcggcaa cggcctctct accggacaaa
     2521 aacagctcat caacttcgcc cgcgccctcg c
//
LOCUS       DASRBV010000143        10980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_566298, whole genome shotgun sequence.
ACCESSION   DASRBV010000143 DASRBV010000000
VERSION     DASRBV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566298"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..4114
                     /locus_tag="VFA02_05420"
     CDS             20..4114
                     /locus_tag="VFA02_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HZU09323.1"
                     /translation="METPRIQSRSGRLDEAGRFAQEGNYTEAMRVYREVFGDKPPAGD
                     YALAYYDTEAAIPSERAHAVEGLRNLAKQFPADSRYAVTLGRVLTYDPKTRTEGMAIL
                     RQYPQVPEAQTALHQAEIWNSQAGNAVATAVKTVAPATPAPAGNPLEAAAYRALNSGR
                     LDEAKDKFQELLSKQPRNAHALAGMGYLYMRQQDFVSAADYFERARAAGEHGLDSAIT
                     NAHFWQKMTDAGNELGSGRAEAAIVDYNAALQIKPNSVDALEGLGNALQQTGDYVQAA
                     QTLDKAVHLDPQRATAWRNLLLVQSSANDSQAVIDANARMPRNVRAQLASDPDYLRVL
                     AQAYQALGRKSDADKVISEALALPFPNEGRDLPVAKQLQYAGLLLTAKRYAPAIRLYR
                     QVVAQDPENAGAWRALIAAQHETNQDEEALTTVGQMPQQVLDQQQNDPAFLVLVGSVY
                     QSRHDYERAAKYLEKALALNPSAPASVVLQLADVYAAQGNTQKAYNIYRSELDQDQSN
                     QQAWRGLLNALHQSGRDREALRQIASMPEAVRLRLEADPGYLQTLAAIQVSTGQSQAA
                     LRNFQRVIDIYHDQNQDIPVGTQIQYGWALLKAGDDQRLYSLVSSLANTAEMTDEQQA
                     DFDNLWAAWSIQRANTLLATGDQRRALTILQTAAQAFPKNTQIYTALAGAYLKAGEAK
                     QAVAIYSSLDMEHATLPQYQGAIGAALAARDLKHAEEWLEGALSQYKTDSTILKMAAQ
                     YEQARGNSDRAAAYYRAALDAMGPGPTRDILSPSGPSATQQLMQLLAPGGRAALSDQT
                     NINQHGVDVSWQNAPGERTPTLGDFAEPGTPDTIATRRDQRDTATLDNFSADAAPLME
                     PEQKRSAIPENAETSAHIEYLRSASTQITTVQPVASQSQKQGRHLNFANDPNPASRLQ
                     AAVQEMSGQAQTPDTGMPAPLEPAPQIGRNLLSTPAPSAKAPELPPLTGPAPLHVVRQ
                     KTEREQIEEQLAMIQGASSAWIGGSSGVDYRSGQPGYDRLANYTTQMESSGMMAPNVR
                     VTVIARPVLLDSGTATSTATFQQGTLPATSTPYLQSAAGIGGEFQLRAPSFGASIGYT
                     PHGFLIENTTGSLYVHPPQSHFTLDFNRAPIMDTQLSYAGLRDLGSKGPSYPGNIWGG
                     VIANAGELQLAFGDARSGWYIQGGGQYITGRHVQDNRRIDGDAGAYWQVWQHADYGTL
                     TLGMNFFGMHYDHNLRYFTYGQGGYFSPAAYMLAGVPFTFNGHRGPRFHYRATGSFGV
                     QAFQENSTPYYPLDPSIQMARNNPYYPENTSVSGNYSLDAEGAYAVAEKWYVGGYLNF
                     NNTRDYAADKVGFYVRYLFRPQPVGESGPTGLFPIQGIRPLQVP"
     gene            4204..6024
                     /locus_tag="VFA02_05425"
     CDS             4204..6024
                     /locus_tag="VFA02_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZU09324.1"
                     /translation="MQRIENYGVIGNMRSIALASTQGAIDFFCFPKFDSPTVFAALLD
                     GKNGGTFCIEPQLENLRTKQLYLPDTNILLTRFLSEAGVVELTDFMPILAGKKGEIYA
                     HQIIRILRVIKGEVRFHVRCAPRFNYARSGHKAVRAENAVRFHPDCPECPEMALHATV
                     PLELDGNDAVCHFTLKPGETATFTFGEANPKIKILDPDEIEGHFQETAHYWREWISRS
                     SYKGRWREIVNRSALVLKLLCSQEYGSVIAAPTFGLPERIGGERNWDYRYTWLRDASF
                     SLYAFLRLGFTEEARGFTEWLRDRLHEDAEHGPLQIMYREDGSQELDESVLENFKGYR
                     NSRPVRIGNGASKQLQLDIYGELMDAIYLSNKYGDSISYDDWRGVWRILDWLGTNWQR
                     PDEGIWEVRGGPRHFLHSRLMCWVAFDRAIRLGRKRSLAAPFADWLAIRDAIYESVFK
                     DFWDDSLKAFVQYQGSKNLDAATLLMPLMRFISPVDPRWLSTLKVIEERLIEDTLVYR
                     YDTQAESIDGMSGSEGSFVACSFWYIECLARAHQVEKARLLFDKMLGYANHLGLYSEE
                     LGSSGEHLGNFPQAFTHLALISAATYLDRALSGNYLSPWH"
     gene            complement(6025..6417)
                     /locus_tag="VFA02_05430"
     CDS             complement(6025..6417)
                     /locus_tag="VFA02_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZU09325.1"
                     /translation="MALHHTGLANAVSIASIIVAATTGDVLIASAMRQIGDLDEIKAH
                     SGLIGAIKAVITNARFFCGVTAMAVSFFSLLFALSHADLSLVAPASASLTVVTNTIAA
                     KIFLKEDVDKRRWIAALCVCAGVVLLAH"
     gene            complement(6417..6866)
                     /locus_tag="VFA02_05435"
     CDS             complement(6417..6866)
                     /locus_tag="VFA02_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZU09326.1"
                     /translation="MTRCTMTFRRYLVLAFVTVSAPLGDSFLDKGMKQIGPVSLAHIT
                     TLFHAVATPWIAGGILLLIGFFASWLTALSWADLTYVLPMTSFANVIVALLAKFWRHE
                     NISLSRWLGIFLITAAVGFVMRGPSYTEHEKPGPARAPAKSPEGACL"
     gene            complement(6926..8401)
                     /gene="hpnJ"
                     /locus_tag="VFA02_05440"
     CDS             complement(6926..8401)
                     /gene="hpnJ"
                     /locus_tag="VFA02_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HZU09327.1"
                     /translation="MPLKTLFLNPPSFENFDGGASSRWPATREIESYWYPVWLAYPAG
                     MLEGSRLLDAPPHHVSAQETIEIAKGYEFLVLFTSTPGWTGDQKLAEAIKRANPDIRI
                     AFVGPPVTTSPDRALQECEVIDFVCRREFDYSVVEYANGKPLHEILGISYRKDGKVVH
                     NPDRPQVDNLDAMPWVTDVYARDLDVTKYNVPFLLHPYVSLYSTRGCPAQCTFCLWPQ
                     TLSGHAWRKRSTDDVAAEMAHAKELFPHVKEYFFDDDTFNIQKARTIELCSKLKPLGL
                     TWSCTSRVTTDRETLKAMRDAGCRLLIVGYESGDPQILKNIKKGATVERARDFTRDCH
                     DLGLTIHGDFILGLPGETKESIRNTINFAKGLDVETIQVSIAHAYPGTEFYEFAKEHG
                     FIINNGQMVDDQGHQLAHIEYPGLPKEYVLEMVHRFYDEYYFRPKAAWRVVSKAIANR
                     DLPRLYQEAKSFLKLRAQRNKIVKESKQQKSNESKPVSAEV"
     gene            complement(8489..9790)
                     /locus_tag="VFA02_05445"
     CDS             complement(8489..9790)
                     /locus_tag="VFA02_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09328.1"
                     /translation="MKPLHTGWIALGLTWFMSAQASAQVSLYTVVDMALRNSTAVRIA
                     QADVMRAAAALTETKDAYIPNLMLGSSLGYSYGFPLGEPSVFNVTSQSLLFTFSQPDY
                     VRSARAALKAAEYSLKDSRQQVILDTALDYVQLDKIQNELAALGQEKQYADKLVSIEQ
                     DRVFAGVDNRMELTKAELTAAQISLKRIHLQNDAELLRQQLSHLTGLAPSSFITNSAS
                     IPSAPDFVQANDLPQRANTLNAGIQAAQANAKSKMYIAFGDSRQIFRPQLSFGADYSL
                     FAKFNNYDQYFRSFQYNNFNIGIQIRVPLFDASLKAKQQRSAADSAHALAEADQTRNN
                     VNEQVLQMQKSLAELTARQRVAQLQAELAQEHLEAISTQLNGSAPGATLTPKDEQQAR
                     IQERQRYEDVLDANFDVFRTELNLMRSIGTIEDWAKSGPKQ"
     gene            complement(9837..10805)
                     /locus_tag="VFA02_05450"
     CDS             complement(9837..10805)
                     /locus_tag="VFA02_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09329.1"
                     /translation="MKISFRILTLALLLCAIAAARADVLDSVRGDLNHGRANQGLDAL
                     DQIIAQQPRNAEAYNLQCRIYMQEERWDNAIRACKSAVDLAPGNSNYHLWLGRALGEK
                     ADRVSFITAFRMARQVRQEFETAAKLDPHNAEALSDLGEFYVDAPAIVGGGMDKAGTI
                     AQQLDAFAPDRAHYIRARMAEGQRDYATAEQEYKAAIAGSKDPADAWMDLASFYRRRQ
                     RWDDMTQAVRAGAAADPKHGVALADGASVLIRAGRDLPLARQLMEQYLASPNKSEEAP
                     AFRVQVQLSKLLAVLGDKQGAQEQIAAAQGLARDYQPATQATNSGR"
BASE COUNT         2385 a         3041 c         3223 g         2331 t
ORIGIN      
        1 ggaggaagtc acaaaaattt tggaaacgcc tcgcatccag tcgcgctccg ggcgtctgga
       61 tgaagcagga cgcttcgccc aggagggaaa ttacaccgag gccatgcgcg tttaccgcga
      121 ggtctttgga gacaaacctc ctgcaggcga ctatgcgctg gcttactacg acactgaggc
      181 cgccattccc tccgagcgcg cccatgccgt ggaagggctg cgtaatctgg caaagcaatt
      241 tcctgcggat tcacggtatg ccgttactct gggccgtgtg ctgacgtatg atccgaagac
      301 ccgcacggaa ggcatggcga ttcttcgtca gtacccgcaa gtgccggagg cgcagacagc
      361 actgcaccag gctgagatat ggaattcgca ggcgggaaat gcagtcgcaa cggcggtaaa
      421 gacagtcgct ccagctactc cggcccctgc cggcaaccca ttggaagccg cggcttatcg
      481 tgcgctgaac agtggccgtc tggatgaggc aaaagacaaa tttcaggagc tgttgtcaaa
      541 gcagccgcgc aatgctcacg ctcttgcagg aatggggtat ctctacatgc ggcagcagga
      601 ctttgtttca gccgccgatt attttgagcg tgcccgcgcc gccggagagc acggcctcga
      661 cagcgccatc accaacgccc acttctggca gaagatgacc gatgcaggaa acgagctagg
      721 ctcgggccgc gcagaagctg cgattgtgga ctacaacgcc gcgctccaga tcaagcccaa
      781 tagtgttgat gcgcttgaag gactcggcaa tgccctgcag cagaccggcg actatgttca
      841 ggcagcgcaa actctggaca aagccgtgca tcttgatccg cagcgcgcaa ctgcatggcg
      901 gaacctgctc ctggtacagt cttcggccaa cgattcacag gctgttatag atgcgaatgc
      961 gcggatgccg cgcaatgtac gcgcgcagct tgccagcgat ccggattatc tgcgcgtgct
     1021 ggcacaggcg tatcaggctt tgggcagaaa atcagatgca gacaaggtga tctctgaggc
     1081 actggcactg ccttttccga acgaaggacg cgacctgcct gtggcaaagc aattgcagta
     1141 tgcaggactt ctgctgactg cgaagcggta tgcacccgcc atccggcttt accggcaggt
     1201 ggttgcgcag gacccggaaa atgccggagc atggcgcgcg ctgattgcgg cccaacatga
     1261 aacaaaccag gatgaagagg cgttaacgac ggttggacag atgccccagc aggtgctgga
     1321 ccaacagcag aacgatcctg cctttctggt gctcgttggc tcagtgtatc aatctcgtca
     1381 tgactatgag cgtgcggcga aatatctgga gaaggcgctg gccctgaatc cgagtgcgcc
     1441 ggcgagcgtg gtcctgcagc ttgccgatgt ctacgctgcc caggggaaca cccaaaaggc
     1501 atataacatc tatcgcagcg agcttgacca ggaccagtcg aaccagcagg catggcgcgg
     1561 actgctgaat gctttacacc agtcgggacg cgaccgggag gcccttcgcc agattgcttc
     1621 catgccggaa gctgtacgcc tgcgtcttga agccgacccc gggtatctgc aaacgctggc
     1681 tgcgatccag gtttctacag ggcagtcgca ggccgcactc agaaatttcc agagggtcat
     1741 agatatttat cacgaccaga accaggacat tcctgttggt acccagatcc agtacggctg
     1801 ggccctgttg aaagccggtg atgaccagag gttgtattcg ctggtttcaa gtcttgccaa
     1861 cacggcagag atgaccgacg agcagcaggc ggacttcgac aatctgtggg cagcgtggag
     1921 catccagcgc gccaatacac tgcttgctac cggagaccag cgccgtgccc tgacaattct
     1981 gcaaacggcc gcgcaggcgt ttccaaagaa cacgcagatt tacaccgcgc tggcaggcgc
     2041 atatctcaaa gcgggagagg caaagcaggc ggtcgccatc tactcgtcac tggatatgga
     2101 gcacgccaca ctgccgcaat atcagggtgc gattggggcc gcgcttgctg cccgtgacct
     2161 gaagcacgcg gaggaatggc tggagggggc gctcagccag tacaagaccg attccacgat
     2221 tctgaagatg gccgcgcagt atgagcaggc gcgcggcaat tcagatcgcg ccgcggccta
     2281 ctaccgcgct gctcttgatg cgatggggcc cggcccgacg cgcgacattc tttccccatc
     2341 cggcccctcg gccacacagc aattgatgca gttgcttgct cccggcggac gcgcagccct
     2401 ttcagaccag accaacatca accagcatgg tgtggatgtt tcctggcaga atgctcccgg
     2461 cgaacgcact cctactcttg gcgatttcgc ggaaccgggc acgccggaca cgattgcaac
     2521 acgcagggat cagcgggaca cggcgacgct ggacaatttc tccgcagatg cagccccgct
     2581 gatggaacca gagcagaaga ggagcgccat accagagaat gcagaaactt ccgcccatat
     2641 cgagtatctg cgctctgcct ccacgcaaat tacgaccgtg cagccggttg cttcgcaatc
     2701 gcaaaaacaa ggccgccatc ttaattttgc caatgacccc aatccagcca gccgtctcca
     2761 ggccgcagtg caggagatga gcggtcaggc gcagacgcct gatacgggga tgccagcccc
     2821 cctcgaaccc gctcctcaga tagggcgaaa tctgttgagc acacccgcgc catctgcaaa
     2881 agcacccgaa cttccaccgc ttaccggccc tgctccattg catgtagtgc gccagaagac
     2941 cgagcgcgag cagattgaag agcagcttgc gatgattcag ggcgcttcga gcgcctggat
     3001 tggcggctct tcgggtgtgg attatcgcag tggtcagccc ggatacgacc gtctggccaa
     3061 ctatacgacc cagatggaaa gctcaggcat gatggcgccc aatgtgcggg tgactgtcat
     3121 cgcgcgccct gttctgcttg attccggaac agctaccagc accgcaacct tccagcaagg
     3181 gaccttgcct gcgacttcaa ccccatatct tcaatccgcc gcaggcatcg gcggagagtt
     3241 ccagctacgg gcgccctcct ttggggcgag cattggatac acgccgcatg gcttcctgat
     3301 tgagaacacc accggcagtc tgtacgtcca tccgccgcag tcacacttta cgctcgactt
     3361 caaccgcgcg ccgataatgg acacgcagct ttcctatgcg ggactgcgtg atcttggcag
     3421 caaagggccg agttatcctg gcaatatatg gggcggagtc atcgccaatg caggagaact
     3481 gcaactggct ttcggtgacg cccgctcagg ctggtacatt cagggcggcg gccagtacat
     3541 taccgggaga catgttcagg acaaccgccg tatagacggc gatgcaggcg catactggca
     3601 ggtctggcag cacgcggatt acggaacgct tacccttggc atgaatttct tcggtatgca
     3661 ctatgaccac aacctgcgct acttcaccta tggccagggt ggatacttca gtccggcggc
     3721 ctacatgctt gcgggcgtgc cgttcacctt caatggacat cgcggaccgc ggttccatta
     3781 ccgcgctacg ggaagctttg gcgtgcaggc gtttcaggaa aactcgacac cttattatcc
     3841 gctggatccg tctattcaga tggcacgtaa taatccttac taccctgaga acaccagcgt
     3901 gagcggcaat tacagtttag acgcagaagg cgcgtacgct gtcgccgaga agtggtacgt
     3961 aggcggttat ctcaacttca acaacacacg cgactatgcc gcggacaagg tcggctttta
     4021 cgtgcgctat ctgttccgtc cgcaacctgt gggagagagc ggaccaacgg gactgtttcc
     4081 cattcagggc atccgtccgc ttcaggtacc gtaattgaaa gcagaagcca gcaactccgg
     4141 cccgtaaacc ttttcgggcc atgcacatct aagcatgggg agtgggaagg aaagaccatc
     4201 ctcatgcagc ggattgaaaa ttatggcgta attgggaaca tgcgctccat cgcgctggca
     4261 agcacgcaag gtgcaattga cttcttctgc tttcccaaat tcgactcgcc tacagtattt
     4321 gctgcgttgc tggacggcaa gaatggcggg acgttctgca ttgagccaca gcttgaaaat
     4381 ctgcgcacca aacaactcta tctgccagac acaaatattt tgctgacgcg gtttctgtcg
     4441 gaagcaggcg tcgtcgaact cacggatttc atgccgattc tggccgggaa aaaaggcgaa
     4501 atttatgccc accagatcat tcgcatcctg cgcgtgatta agggtgaggt caggtttcat
     4561 gtgcgctgcg cgccgcgttt taactacgcg cgcagcggac ataaggccgt ccgcgcagag
     4621 aacgcagtgc gctttcatcc tgattgcccg gagtgtccgg agatggccct gcatgcaact
     4681 gtcccgctgg aactcgacgg taacgatgcg gtgtgccact tcacgctgaa gccaggtgag
     4741 acggcgacat ttaccttcgg agaggccaac cctaagatca aaattcttga tccggacgag
     4801 atcgaaggcc attttcagga gacggcccat tactggcgcg aatggatcag ccgttcgagt
     4861 tacaaaggcc gctggcgcga gatcgtaaac cgttcggccc tggtgctgaa attgctgtgc
     4921 agccaggaat acggctctgt gattgccgcg cctacgtttg gcctgccgga aagaatcggc
     4981 ggtgagcgca actgggacta tcgctacacc tggttgcgcg atgcctcctt cagcctctat
     5041 gcctttcttc gcctcggatt cacggaggaa gcgcgcggtt ttactgaatg gctgcgcgac
     5101 cgtctgcacg aagatgctga gcatggcccc ctacaaatca tgtatcgcga ggacggcagc
     5161 caggagctgg acgagtcggt ccttgaaaac ttcaaaggct acaggaattc gcgtccggta
     5221 cgcatcggca atggcgcgtc aaaacaattg cagctcgata tttacggcga actgatggac
     5281 gccatctatc tgtcaaacaa gtatggcgac tcgatttcct atgacgactg gcgcggcgta
     5341 tggcgcattc tggactggct gggaacgaac tggcagcgtc cggatgaagg tatctgggaa
     5401 gtacggggag ggcccagaca ttttctgcac tcccgcctga tgtgctgggt ggcctttgac
     5461 cgcgccatcc gtctgggaag aaagcgctcg ctggccgcac cttttgcaga ctggctggcc
     5521 attcgcgatg cgatttacga gagcgttttc aaggatttct gggacgactc gttgaaagcg
     5581 tttgtgcagt atcagggatc aaagaacctg gatgctgcca ctttgctgat gccattgatg
     5641 cgttttatca gtcctgtaga tccgcgctgg ctgtccacgc tgaaagtgat tgaagagagg
     5701 ctgatcgagg acacgctggt ctaccgttat gacacacagg cagaatccat tgatggaatg
     5761 tccgggtccg agggcagctt tgtcgcctgc tctttctggt acatcgaatg cctggcccgc
     5821 gcccaccaag tggaaaaagc gcggcttctc tttgacaaaa tgctaggcta cgctaatcat
     5881 ttgggacttt attcagaaga gctggggagc agcggcgagc atctggggaa tttcccgcag
     5941 gcgtttacac accttgcact gatcagtgcg gcgacctacc tggaccgcgc attgtcagga
     6001 aattatttaa gtccctggca ttgatcaatg cgccagcagg acaactcccg cgcagacgca
     6061 caaagcagcg atccagcggc gcttgtccac gtcctctttg aggaagattt ttgcagctat
     6121 ggtgttggtc accaccgtga gcgacgcgga agccggagct acaaggctca aatctgcatg
     6181 gctcagcgcg aacaacagag aaaagaagct gaccgccata gcggtgacac cgcagaaaaa
     6241 gcgcgcattg gtaatcaccg ctttgatggc ccctatgagt ccggaatggg ccttgatttc
     6301 atccagatca ccaatctggc gcatggcgga tgcaataagc acatcgcctg tggtggcggc
     6361 aacaataatg ctggcaatgc taaccgcatt ggccagtccg gtatggtgaa gtgccatcac
     6421 aggcaggctc cttccggaga ttttgcagga gcgcgcgctg gtcccggttt ttcgtgttcg
     6481 gtataggacg gccctcgcat gacaaaccct acagcggctg taatcagaaa gatgcccagc
     6541 cagcgggaaa gagagatgtt ctcatgccgc cagaactttg ccagcagcgc cacaatcacg
     6601 ttggcaaacg atgtcatcgg cagaacatag gtgagatcag cccatgacag cgcggtgagc
     6661 cacgatgcga agaagccgat cagaagcaga atgcccccgg caatccaggg cgtagccacg
     6721 gcatgaaaga gagtggtgat gtgcgccagg gataccgggc ctatctgctt catgcctttg
     6781 tcgagaaaag agtcaccaag cggggctgag accgtgacaa aggccaggac tagatagcgc
     6841 cggaatgtca ttgtgcatcg cgtcatggaa aagagaaacc cacatctctt tgagatgtgg
     6901 ggtacccata caactggtga gacttctaaa cttccgcgct tacaggcttg gattcgttcg
     6961 atttctgctg cttgctttct ttcacgatct tattgcgttg cgcacgcagt ttcaggaacg
     7021 atttcgcctc ctgatacaga cgaggcaggt cgcggttggc aatcgctttg cttactacgc
     7081 gccatgccgc cttcggacgg aagtagtact cgtcatagaa gcggtgcacc atttcgagta
     7141 cgtactcctt gggcaatccg ggatattcga tatgggcaag ctggtggccc tgatcgtcta
     7201 ccatctgacc gttattgatg atgaagccat gttctttggc aaattcgtag aattctgttc
     7261 cgggatacgc gtgtgcaatc gagacctgaa tggtttctac gtcaaggccc ttggcgaaat
     7321 taatggtgtt gcgaatggac tcttttgttt ccccgggcag gccgaggatg aagtcgccgt
     7381 ggatggtcag gccgaggtcg tggcagtcgc gggtaaagtc gcgggcgcgc tcgacagtag
     7441 cacctttctt gatgttcttg aggatctgcg gatcgcctga ttcgtatcca acgatgagca
     7501 gccggcatcc ggcgtcgcgc atggccttta aagtttcgcg gtcggtggtg acgcgcgaag
     7561 tgcaggacca tgtcaacccg agcggcttca gcttcgagca caactcgatg gtgcgtgcct
     7621 tctgaatatt gaaggtgtcg tcgtcaaaga agtattcctt aacatgcggg aagagttcct
     7681 tcgcatgggc catctctgcg gcaacgtcat ctgtggagcg tttgcgccat gcatgaccgg
     7741 agagtgtctg cggccagaga cagaaggtgc actgggccgg gcatccgcgt gtggagtaaa
     7801 gcgaaacata gggatgcagc aaaaagggta cgttgtactt cgtcacgtcg aggtcgcgcg
     7861 catagacatc tgtgacccac ggcatggcgt ccaggttgtc cacctggggg cggtcagggt
     7921 tatgcaccac tttgccatct ttgcggtagc tgatgcccag aatttcgtgg agcggcttgc
     7981 cgtttgcgta ttcgacaacc gaatagtcga actcgcggcg acatacgaaa tcaatgactt
     8041 cgcactcctg aagcgcacgg tcgggcgagg tggtcaccgg aggtccaacg aacgcaatgc
     8101 ggatgtcggg attggcacgc ttgatggcct cggccagttt ctggtcaccc gtccagcccg
     8161 gtgtgctggt gaacagcacg aggaactcgt agcctttggc gatctcgatg gtctcctgcg
     8221 ccgagacgtg gtgcggcggg gcatcgagca gccgcgagcc ttcgagcatc ccggccggat
     8281 aggcgagcca caccggatac cagtaggact cgatctcacg cgtagcaggc cagcgtgaac
     8341 tggcgccacc gtcaaaattc tcgaaggagg gcgggttaag gaacagtgtt tttaaaggca
     8401 tgtgcgtatc tttaagttta acatctggtg caggatttcc cggtcaaaag gtgcctttgg
     8461 agctactcct ggtaagccgg tgattttctt actgctttgg gccagatttc gcccaatctt
     8521 cgatggtgcc gatcgagcgc atcagattca gttcagtgcg gaacacatcg aaattcgcgt
     8581 caagtacatc ctcatagcgc tgacgttcct ggatgcgcgc ctgctgttcg tccttaggtg
     8641 tgagggtggc gcctggcgca ctgccattca attgagtaga aatagcctcg aggtgctcct
     8701 gggccagttc cgcctgcagc tgggcaacac gctggcgggc cgtgagttcg gcaaggctct
     8761 tctgcatctg caaaacctgt tcgttcacat tgttgcgggt ctggtctgct tcggccaggg
     8821 cgtgcgcaga atccgcagcg gaacgttgct gtttggcctt gagcgaagcg tcgaagagcg
     8881 gcacgcggat ctggatgccg atgttgaagt tgttgtattg gaagctgcgg aaatattgat
     8941 cgtagttatt aaatttggca aatagactgt agtcggcccc aaaggaaagc tgtggacgaa
     9001 aaatctgccg tgaatctccg aaggcgatat acattttgga ttttgcattt gcctgcgctg
     9061 cctggattcc ggcatttaaa gtatttgccc gttgtggaag atcgtttgcc tgaacaaagt
     9121 caggcgcaga gggaatgctg gcgctgttgg taatgaagct ggaaggcgcc agtccagtga
     9181 gatgagagag ctgctggcgc aaaagctccg catcattctg caagtggatc cgcttcagag
     9241 aaatctgggc ggctgtaagt tcggccttcg tcaattccat gcggttgtct acgccggcaa
     9301 agacgcggtc ctgctcaatg ctcacgagct tgtcggcgta ctgtttttcc tgaccaaggg
     9361 cggccagttc attctgaatt ttatcgagct gtacataatc cagcgccgta tcaagtatga
     9421 cctgctggcg gctgtctttc aggctgtact cagctgcttt gagcgcagcc cgggccgagc
     9481 gaacatagtc cggctgcgaa aaggtgaaga gcagtgattg cgaggtgaca ttaaaaaccg
     9541 acggctcacc cagcggaaaa ccataggaat aaccaagact tgagccgagc atgagattcg
     9601 gaatataggc gtctttggtt tcggtaaggg ctgcggcagc gcgcatcacg tctgcttgtg
     9661 caatgcggac ggccgtgctg ttgcgcaggg ccatgtccac gacggtatac agcgaaacct
     9721 gcgcggatgc ctgggcagac atgaaccatg tcaggcccag cgctatccat ccggtatgta
     9781 acggtttcaa ccgcaaatgt cctctttcag cgattgcaag ttctgatttt atctgcttat
     9841 ctgccgctgt tggttgcctg tgtagctggt tggtaatccc ttgcaaggcc ctgcgccgca
     9901 gcgatctgct cctgggcgcc ttgcttatcg ccaagtaccg ccaacaattt gctcagttgc
     9961 acctgcacac ggaaggccgg ggcttcttca gatttattgg gagaagcgag atactgttcc
    10021 atcaactggc gggccagggg aaggtcgcgt ccggcgcgaa tgaggacaga agctccatcc
    10081 gcgagcgcga caccgtgctt tggatcggcg gctgctcctg cacgcacggc ctgtgtcatg
    10141 tcgtcccatc gctggcggcg gcggtagaag gaagccaggt ccatccatgc gtcggcgggg
    10201 tctttggatc ctgcgatcgc agccttgtat tcctgttcgg cggtggcata gtctctctgg
    10261 ccctcggcca tgcgggctcg gatatagtgg gcgcggtcag gggcaaaagc atcgagctgc
    10321 tgcgcgattg tgcctgcctt gtccattccg ccgccgacaa tcgccggcgc atccacgtag
    10381 aactcgccca gatcagaaag agcctcggca ttatgcgggt cgagtttcgc ggcggtctca
    10441 aactcctgac gtacctgccg cgccattctg aaggcggtga tgaaactgac acggtcggcc
    10501 ttttctccca gggcccgccc cagccacagg tggtagttgc tgttaccggg tgcgagatcc
    10561 accgctgatt tgcaggcgcg gatggcattg tcccagcgct cttcctgcat gtagatgcgg
    10621 cactggaggt tgtacgcctc ggcattccgg ggctgctggg caatgatctg gtccagagcg
    10681 tccagccctt gattcgcgcg cccatgattc aggtcgccgc gcacagaatc cagtacgtcc
    10741 gcgcgcgcag ccgcgatggc gcacagcaac agtgcaagag tcaggatgcg aaaagatatc
    10801 ttcatttact gcaaaatatt tacgacggcc ccgtcgctga gcggctgccc attggtcgtg
    10861 cccagcgcga cggcatcgcc ttcattgagt ccggagagga tttctatctg cgtaatattg
    10921 gatgcgccag tcgtcacctg ggtgcggcga agcactccat ctactatgcg atagacatag
//
LOCUS       DASRBV010000144         6108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_464127, whole genome shotgun sequence.
ACCESSION   DASRBV010000144 DASRBV010000000
VERSION     DASRBV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464127"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFA02_05455"
     CDS             <1..352
                     /locus_tag="VFA02_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU09330.1"
                     /translation="MYYELLPELKAVPEVLEHIEDAHGRVPFAVVSGSTRHSVTASLA
                     ALNLLEKFDTLVCAGDYKRSKPHPECFLLAAERLGVAPENCLVFEDTEMGIQAATSAG
                     MASVKVPMPWERTE"
     gene            935..2266
                     /locus_tag="VFA02_05460"
     CDS             935..2266
                     /locus_tag="VFA02_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU09331.1"
                     /translation="MGLVPLIAATYFMVSGGPYGIEDILSGAGYSKAFLILLVFPFLW
                     SFPTTLMLGELSSAIPAEGGFYVWVRRALGPFWGYQEAWLSLTASIFDMAIYPTIFVL
                     YLGRLFPAWTAGHRGMMWELALVAACCVWNLCGAPAVGKGSVALFLALLSPFVVLVIL
                     GFLHGPHWRGETTAFTGGSISTAVIVAMWNYMGWDNASTVAEEVENPRRNYPLAMLLC
                     AALVAISYLLPVAAAGYAGISVTQFSTGSWADAASTLGGPLLAGAVVLCGTINGLGMF
                     NALVLSYTRLPMVLAQDGMLPSCFALKNRRGVPWVAVIVCGAAWAAALGFRFEELISI
                     DLILYGSSLLLEFIALAVLRFTEPALPRPFRAGNKFMACTLGLPPALLILYVMYSARG
                     ERMAHMPALLFGLLIALAGPVFYWLSRKCWTPEMDCAASRTAIIHSKNKSY"
     gene            2282..3802
                     /locus_tag="VFA02_05465"
     CDS             2282..3802
                     /locus_tag="VFA02_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZU09332.1"
                     /translation="MPTAHPLPTTLGDLRKSQEFSESRLAARSVKDELRENLIARLKS
                     RETIFPGIVGYEDTVVPQIVNAILSRQNFILLGLRGQAKSRILRALTGILDDQMPFIA
                     GCEIRDNPYKPLCRRCRDLIAEKGDATPIDYMGRDERYVEKLATPDVTVADLIGDLDP
                     IKAARGGHHLASELTMHFGLLPRANRGIFAINELPDLAGKIQVALFNIMQEGDVQIKG
                     YPVRLALDVALVFSANPEDYTARGKIVTPLKDRIGSEIRTHYPETLEEGIAITAQETW
                     SNRGLGTLEVPHYLREIVEQIAFTAREDKKVDKRSGVSQRLPISTMDLVISNAERRSL
                     LHGEPLIMPRIGDIYASLPGITGKLELEYEGEMRGADTVVREILRAAIAKIFDKYFGG
                     TNTQQIEQWFNLGGTIKFDDDQSSKSIASELKQIQGLVEKLAPLSITAKSDPEHLVAA
                     AEFLLEGMYAHHRISRTEERAFTAAEKPQRSTMRPERDLERDLEDWPQRRTRRGFN"
     gene            complement(3994..5451)
                     /locus_tag="VFA02_05470"
     CDS             complement(3994..5451)
                     /locus_tag="VFA02_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU09333.1"
                     /translation="MPRKYVLFASMAVCATLHCVAQSPSIQDRLQRLMNTSTGAWTAD
                     QIATMEKLRDAAMQDPYALDELRHLTDNIGPRLSGSPQAQKAVEYVAAEMKALGADVH
                     LEKTMVPHWVRGVETGELVAWPGMAEGTTQKIVLTALGGSVATPANGVTADVVVADSF
                     DDVKKLPEGAVKGKILLLNEKFDKRLAAQGDGLAAYGQAVVYRGAGPSVAASLGAVAV
                     LVRSVGNADFRLPHTGATFYAEGLPKIPAAAVTAEDADLLKDLSQQGPVRMHLTLTPQ
                     NLPKVPSYNVIADWKGSEHPEQVVIVSGHLDSWDLATGAIDDGAGIAVSMQAIHLLQK
                     LGIHPKRTVRFVAWMDEEQGGSGAETYSKEHAGDMNNHTGAIESDLGAGHPIGMMAAG
                     NPQLEEWLRPVAHALAPIGATVVTMSPDAGEDISFLKGVPQFAPNQDSRFYFNYHHTA
                     ADTFDKVNPQELNENAAVMTVLAYALADSGTPAPR"
     gene            5729..>6108
                     /locus_tag="VFA02_05475"
     CDS             5729..>6108
                     /locus_tag="VFA02_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09334.1"
                     /translation="MYNECRHILTNGNRCQSPALRHHDFCYFHSVHRAQPPVEATFDL
                     NLPPLEDPDAIQLALSQLAQALAANKIDIRRARALAMVLRIASANFRHTIAGQRLLQK
                     KSEPQQDPAPALYEQQMVRETCSHS"
BASE COUNT         1255 a         2109 c         1493 g         1251 t
ORIGIN      
        1 catgtactat gagctgctcc ctgaattgaa ggcagtacct gaggtcctgg agcacatcga
       61 agacgcccac ggcagggtcc cctttgccgt cgtttccgga agcacccgcc attctgtgac
      121 ggcatcgctc gctgcgctca atcttctgga aaaattcgac acgctggtct gcgcaggcga
      181 ttacaaacgc agcaaacccc acccggaatg ctttctcctc gcagccgaac gcctcggagt
      241 tgcccctgaa aactgtctcg tctttgaaga cactgagatg ggcatccagg ccgcaacctc
      301 cgcaggcatg gcctcagtca aagtccccat gccctgggaa cgaacagaat gacaagtatg
      361 taggacccgt cattctacga ccgaagggag gaagaatccc agcgatgtgc ggctcagcca
      421 atcccgcctt tcccggcgtt gaatcatccc gaccatccgg ttttgaaggg gcacggcttt
      481 cagccgtgcc gttgcatcct gcaaacatgc ggctttagcc gctgagggat cgcctttcca
      541 gctttgaaat catacattcc ttcagaccag cccctggggg aactctcacg caatccggta
      601 agccgactcc cgccattccg acgatcgctc tattgccgtc attctacgat gcggataact
      661 gcggcgctcc ttgatgacct cgccgccatt ccgacgatcg ctctattgcc gctatttctg
      721 acgacggata catagccccg tcattctgat gaccgaagag aggaagaatc tcagcgatgc
      781 gagtctctgg ctgatagtct gactgctgac cgcttcttag ctcgcattcc atacaattct
      841 ccttcccatc gaattgcatc ttgcacaata atttcttcga gagggaccag cagtagccac
      901 taccatcaca tctccgaaat cccgcacccg ccgtatgggt cttgttccct tgatcgcggc
      961 aacctatttc atggtttcag gcggacctta tggtatcgag gatatcctca gcggagccgg
     1021 atattccaaa gcgttcctca tcctccttgt cttccccttt ctctggagct ttcccacgac
     1081 cctcatgctc ggcgaactct cgagcgccat tcccgccgaa ggcggattct atgtctgggt
     1141 gcgacgcgcc ctcggcccct tctggggata tcaggaagca tggctctccc tcacggccag
     1201 catctttgac atggccattt atcccaccat cttcgtgctc tatctcggca ggctctttcc
     1261 cgcatggaca gccggccacc gcggcatgat gtgggagctg gcgctcgtcg ccgcctgctg
     1321 tgtctggaac ctctgcggcg cgcccgccgt cggcaaaggc tccgtcgcgc tgttcctcgc
     1381 attgctcagc ccctttgtcg ttctggtcat cctgggattt ctccacggtc cacactggcg
     1441 tggcgaaaca acagcattca cagggggaag catcagcacc gccgtaatcg tcgcgatgtg
     1501 gaattatatg gggtgggaca acgcctccac tgtcgccgaa gaagttgaaa atccgcgccg
     1561 caattatccc ctggccatgc tgttgtgtgc cgcattggtg gccatctcct atctgcttcc
     1621 cgtcgcagca gccggatatg ccggcatttc cgtcacgcaa ttctctaccg gctcatgggc
     1681 cgatgcggcc agcacactcg gcggcccgct cctggcaggg gccgtcgttc tctgcgggac
     1741 catcaatggc ctcggcatgt ttaacgctct cgtgctctct tatacccgcc tgcccatggt
     1801 ccttgctcag gacggaatgc tgccctcatg cttcgccctg aaaaaccgtc gcggcgtccc
     1861 ctgggtcgcc gtcatcgtct gcggagcagc atgggcggcg gcacttggct ttcgcttcga
     1921 agagctgatt tccattgacc tcatcctcta cggcagcagt ctccttctcg aattcattgc
     1981 cctcgccgtc ctgcgcttca ccgaacccgc cctgccgcgc cccttccggg ccggaaataa
     2041 attcatggcc tgtacgctcg gcctcccgcc cgccctgttg attctttacg taatgtattc
     2101 cgcccggggc gagcgcatgg cccacatgcc tgccctgctc tttgggcttc tcatcgccct
     2161 tgcaggccct gtcttctact ggctttcccg taagtgctgg acgcctgaaa tggattgcgc
     2221 cgcttcccgc accgccatca tccattcaaa aaacaagtca tactagtaaa caggagccat
     2281 aatgcctacc gctcatcctc tgccaacgac actcggtgat ctgcgtaaga gccaggaatt
     2341 ttcagaatcc cgcctcgctg ctcgcagcgt gaaagatgag ctgcgcgaaa acctcatcgc
     2401 ccgcctcaaa tcgcgcgaga ccatctttcc aggcattgtc ggatatgaag acacggtcgt
     2461 gccgcagatt gtcaacgcca ttctttccag gcagaacttc atcctgctcg gtctgcgcgg
     2521 acaggccaag agccgcattc tgcgcgccct tacaggcatt ctcgatgacc agatgccctt
     2581 catcgccgga tgcgaaatcc gcgacaaccc ctacaagccg ctctgcaggc gctgccgcga
     2641 cctcatcgct gaaaaaggcg acgccacccc cattgactac atgggccgcg atgagcgtta
     2701 cgtcgaaaag ctcgccacgc ccgacgtcac cgtggccgac ctcatcggcg acctcgaccc
     2761 tatcaaggcc gcacgcggcg gacatcacct cgccagcgag ctgaccatgc acttcggcct
     2821 gttgccccgc gccaaccgcg gcatcttcgc catcaacgag ctgcctgacc tcgccggaaa
     2881 aatccaggtc gcgctcttca acatcatgca ggaaggcgac gtgcagatta aaggctaccc
     2941 cgtccgcctc gcgctcgatg tcgcgctcgt cttcagcgcc aatcctgaag actacacagc
     3001 gcgcggcaaa atcgttaccc cgctgaaaga ccgtatcggc tcggagatcc gcacgcacta
     3061 tcctgaaaca ctcgaagaag gcatcgccat cacggcacag gaaacatgga gcaaccgcgg
     3121 cctcggcacg cttgaggtcc cgcactactt gcgcgaaatc gtcgagcaga tcgccttcac
     3181 tgcgcgcgaa gacaaaaagg tagacaagcg cagcggcgtc agccagcgcc tgcccatctc
     3241 cacaatggat ctcgtcatct cgaacgccga acgccgttcc ctgcttcatg gcgaaccgct
     3301 catcatgcct cgcatcggag acatctatgc ctcactgcct ggaatcacag gaaagctgga
     3361 gcttgaatac gaaggcgaaa tgcgcggcgc agacaccgtc gtccgtgaga ttctccgcgc
     3421 cgctatcgcc aaaatcttcg acaaatactt cggcggcacc aacacacagc aaattgaaca
     3481 gtggttcaat ctcggcggca ccatcaagtt cgatgacgac cagtcctcaa aatccatcgc
     3541 atctgagtta aagcagattc agggactcgt cgaaaagctc gctccgctca gcattacagc
     3601 caaatctgac ccggagcatc tggtcgccgc cgccgaattt cttctggaag gcatgtatgc
     3661 acaccatcgc atcagccgca cggaagaacg ggccttcacc gccgccgaaa aaccacagcg
     3721 cagcaccatg cgcccggaac gcgacctcga acgcgacctg gaagactggc cgcagcgccg
     3781 gacccgtcgc ggctttaact aagacaacgc tggcattccg ggcagagagt cgtcatccta
     3841 acggccaata atatatcccg tcattctgac gaccgaaggg aggaagaatc ccagcgatct
     3901 gagcgttagg ccaatttcgc ttcaccgtgg tccacgaaaa gtggtgacgt cactccaggc
     3961 gagcgcacaa tctttagctg ctttgggccg tccctatctc ggcgcgggcg ttcccgagtc
     4021 ggccagcgca taggccagca ctgtcatcac cgctgcattc tcattcagtt cttgcggatt
     4081 gactttatcg aaggtgtccg ccgctgtgtg gtggtagttg aagtaaaacc ggctgtcctg
     4141 gttcggagca aactgcggca ctcccttcag aaatgaaatg tcctctcctg catccggact
     4201 catcgttacc accgtcgccc caatcggcgc cagcgcatgc gccactgggc gcagccattc
     4261 ctccagttgc ggattgcccg ccgccatcat tccaatggga tgcccggctc ccaggtccga
     4321 ctcaatcgcg ccggtgtggt tgttcatatc gcctgcgtgc tctttggaat aggtctccgc
     4381 gcccgacccg ccctgctctt cgtccatcca tgccacaaaa cgcaccgtgc gtttgggatg
     4441 aatacccagc ttctgcagca gatgaatcgc ctgcatggaa acggcaattc ccgcgccatc
     4501 gtcaatcgcg cctgtcgcca ggtcccatga atccagatga ccggaaacaa ttaccacctg
     4561 ctccggatgc tcgcttccct tccagtcagc aatcacgtta tagctcggta ctttgggcaa
     4621 attttgcgga gtcaacgtaa ggtgcatccg caccggccct tgctgtgaca ggtctttcag
     4681 caggtcagca tcttccgccg tcactgccgc cgccggaatt tttggaagcc cctcagcata
     4741 gaaggttgct ccggtgtgcg gcaaacggaa gtcggcgttt cccacagacc gcaccaggac
     4801 tgcaactgcg cctaacgaag cggcaacact cggcccagct ccgcgataga ccactgcctg
     4861 tccataggcg gcaagcccgt ctccctgcgc cgccaggcgc ttgtcaaact tctcattcag
     4921 cagaaggatt ttgcctttga ctgcgccttc cggcagcttc ttcacatcat cgaaggaatc
     4981 cgccacaacc acgtccgccg tcaccccatt cgccggagtc gccacactgc ctcccaacgc
     5041 ggtcagcacg atcttctgcg ttgtaccctc agccatgcct ggccatgcaa ccagctcgcc
     5101 cgtctccaca ccacgcaccc agtggggcac catggttttt tcaagatgca catccgcgcc
     5161 cagcgccttc atctcggcgg caacatactc gaccgctttc tgcgcctgcg gagaaccact
     5221 cagccgcggt ccgatattgt ccgtcaggtg tcgcagttca tccagcgcat aggggtcctg
     5281 catcgccgca tcgcgcagct tttccatggt cgcaatctgg tctgccgtcc acgcgccggt
     5341 ggatgtgttc atcaaccgct gcaatctgtc ctgaatcgaa gggctttgcg caacgcaatg
     5401 aagcgtcgcg cacacggcca tagaggcaaa aagaacgtac tttctgggca taggcgcatc
     5461 ctaccatgcc agacgcacgg accgttcccg tccctttccc cacgcctgtc aagcatcacg
     5521 aaagtaacaa agccctctaa ctccttccca cagcaggaga tagccaacaa cccgcaatgc
     5581 cggaacaccc ctctcaacca gctatactga acatagaggg taaaaattct cccaaagggc
     5641 ccgcccacaa agcgggcctt gcgcattttt cagctaaccg gcttttcagc tacccgcttt
     5701 tcagccaccg caacgcaaag gaaccgccat gtacaacgag tgccggcaca ttctcaccaa
     5761 cggaaaccgc tgccagtccc cggccctgcg ccatcacgac ttctgctact tccactccgt
     5821 ccaccgcgcc cagccccccg tcgaggccac tttcgacctc aacctgcccc cgctcgaaga
     5881 ccccgacgcc atccagctcg ccctctccca gctcgcccag gccctggccg ccaacaagat
     5941 tgacattcgc cgcgcccgcg ccctcgccat ggtcctgcgc atcgcctccg ccaacttccg
     6001 ccataccatc gccggccagc gcctgcttca gaaaaagagc gaaccacaac aagaccctgc
     6061 cccggccctc tacgaacaac agatggtccg cgaaacctgc tcccactc
//
LOCUS       DASRBV010000145         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_362478, whole genome shotgun sequence.
ACCESSION   DASRBV010000145 DASRBV010000000
VERSION     DASRBV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362478"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFA02_05480"
     CDS             <1..1112
                     /locus_tag="VFA02_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZU09335.1"
                     /translation="SCQRTMLDLLREMVEIESPSDDKAAVDRCSSFLAKQCKSLGGRV
                     RLHKQKAFGDLLEARFGPAAGKSRRKPILLLGHLDTVWPLGTLKSMPFRMAQERAWGP
                     GVLDMKAGVVMALTALSILQHQKRLKRPVIMLLNSDEEIGSTVSRPVTEELALECEAV
                     YVLEPAQGLDGAYKTARKGIGNYRVSVKGVAAHSGVDFERGHSAIAELARQVVRITGF
                     TELERGTTVNIGTIQGGTRSNVVAAEAHADVDVRIVRAREAGRVEKQFRSLRVFDNKC
                     SLHVEGGLNRPPMERTRGTVALYKRATALARALGVDLKEAATGGGSDGNFTSALGVPT
                     LDGMGAVGEGAHAAHESILLEHLAPRTALLAAMLE"
     gene            1120..1266
                     /locus_tag="VFA02_05485"
     CDS             1120..1266
                     /locus_tag="VFA02_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09336.1"
                     /translation="MLKFIEFALLAVSLLIIFGLVAVSPRTQASVPEIAESTEAEAPV
                     LPKL"
     gene            1482..2987
                     /gene="gltX"
                     /locus_tag="VFA02_05490"
     CDS             1482..2987
                     /gene="gltX"
                     /locus_tag="VFA02_05490"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZU09337.1"
                     /translation="MEKKETRVRFAPSPTGLLHVGNARTALYNWLVARHTGGKFVLRI
                     EDTDVERSREEHEKQLIEDLRWLGLDWDEGPEMGGPHGPYRQSERLEIYRKHTEQLLT
                     EGKAYRCFCTAEELEAERQKAIVEHRPQVYSGKCRSIAKTNSDGRAAAGEPFAVRLRI
                     PDKPLRFHDMVRGEVEFPNETVSDPILVRSSGIPVYNYVVTVDDALMEITHVIRGDDH
                     ISNTPKQVAIYEAFGWPVPEFAHLSTILGSDRERLSKRHGATSIASFREMGILPEALV
                     NYLALLGWGAEGGTRETFTREELIKEFRLERVTASPAVFDFDKLHWLNRHYLKLSSPA
                     HVLDLAWPYFAKRQWLPARDVADKQTVDWFGKLLTLFLPAVDRLEQLPEKAQFIFHFD
                     AMAAKANEENTQILQSGSAQKVLGAFAGCVRAHHGVVTPELFKSWMNEVKSETGRKGK
                     ELFHPVRIALTGAHSGPEFDKLIPLFEEGSALALPEHVLSVRERIEQFIPA"
     gene            2984..3541
                     /locus_tag="VFA02_05495"
     CDS             2984..3541
                     /locus_tag="VFA02_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09338.1"
                     /translation="MSVRLEVSFSHSWTVKVLKERPLIAPARQFVYPRQAEEVERGAL
                     ELLIQPADGAKFLATCAAGFADPSAPTGVWSCPHPDWLCAVSGGYAYLIHTLQPETWQ
                     MVPYRPVLGIRALPAQQLLLFIGHHSILAWGREGLAWQSERLSWEGVQVTGIVDDALH
                     GLGWDLMTDKDVPFALDLRTGKRIG"
     gene            complement(3435..3818)
                     /locus_tag="VFA02_05500"
     CDS             complement(3435..3818)
                     /locus_tag="VFA02_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZU09339.1"
                     /translation="MAQAIYLPILKFCLRHRWLTIAVNVIFLLVTISLATRLGSQFMP
                     PLFEGSALYMPIALFPEVLEHFKSTGPGWQTRINQTLKSAIRKKKTGLFSQSAFPSSN
                     PTQTAHLCRSSGPSRGRGVHRPQCL"
     gene            complement(3850..>5596)
                     /locus_tag="VFA02_05505"
     CDS             complement(3850..>5596)
                     /locus_tag="VFA02_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZU09340.1"
                     /translation="CYFCKNNCLRTFIDVDVNGEHTDRAATPEEVTAAVTNIQNLPSL
                     EQIQANLPEAEAHTHGSSCSTHASPFKAKAEPLVQIQLAPGSSKVVELPKPVEFQPRK
                     TKVPLRKGEQRLIIATCEKGTVEDLDEMKDIKADIDKIKAANPNFVDIAAHDVFRHRE
                     VQVVADPVPNAKGLFVSKAIKERAALMEHRKDFRIGIPRLLNIYTYAPLFNAYFASLG
                     IKAENIIYSDYTTPELYRAGASRGAIDPCYPSKIGIAHVYNLLTTKHNKKPLNAVWFP
                     MYDVLHTPLVNLTGSNACPTVTATPEVVKAAFTKENDIFAENGISYLDPILNLQDQKI
                     CADQMYKAWAPILGLSREENDRAIEVGFTALLDYEAEIRRKAREVLDQLEREDRIGIV
                     MLGRVYHHDPGLNHEIMEEFQKLGYPVFSQSTLPLDEDLLERLFGDEVRAGVITHPLD
                     ISDVWKNRYSTSTNHKVWAAKFTARHPNLVALEVSSFKCGHDAPIYGVIEGIIEESGT
                     PYFSFKDLDENKPSGSIRIRVETIDYFLRRYREDIIQRRIKEKDIEAQLAKLEQELRN
                     RKYIQTEQEMAIAGD"
BASE COUNT         1160 a         1462 c         1721 g         1253 t
ORIGIN      
        1 aatcgtgcca gcgaaccatg ctggatctgc tgcgcgagat ggtcgaaatc gaatcgccca
       61 gcgacgataa ggccgcagtg gaccgctgct cttcctttct cgcaaagcaa tgcaaatcac
      121 tcggagggcg agtgcggctt cataaacaaa aggcattcgg cgacctgctg gaagcgcggt
      181 ttggccccgc agccggaaaa tctagacgaa agcccatcct tttgctggga catctggata
      241 cggtttggcc tctgggaaca ctaaagagta tgccctttcg catggcccag gaacgggcgt
      301 gggggccggg tgttttggac atgaaggccg gagtggtaat ggccctgacg gctctttcta
      361 ttttgcaaca ccagaagcgg ctgaagcgcc cggtgatcat gctgctgaac agcgatgaag
      421 aaatcggcag cacggtatcg cgtcctgtca ctgaggagct ggcgcttgag tgtgaggccg
      481 tttatgttct ggaaccggcg cagggactgg acggcgcgta taagactgcg cgcaagggca
      541 tcggtaacta tcgcgtaagc gtaaaaggcg tggccgcgca cagtggagtg gattttgagc
      601 gcggacacag cgccatcgct gaacttgcac ggcaggtcgt gcgtataaca ggttttaccg
      661 aattggagcg cgggaccacg gtgaatattg gaaccattca ggggggaacg cgatctaacg
      721 tagtagcagc cgaggcccac gccgacgtgg atgttcgtat tgtgcgggcc agagaggcgg
      781 ggcgcgtgga gaaacaattt cgttctttgc gcgtctttga taacaagtgc agtctgcatg
      841 tagaaggcgg actgaaccgt cctccgatgg agcgcacgcg cggaacggtg gcgctctaca
      901 agcgggccac agccttggcc cgcgcgcttg gagttgattt gaaggaagca gcgacgggag
      961 gcggttcaga cggcaatttt acttccgccc tcggcgtgcc cacattagac ggaatgggag
     1021 cagtgggaga aggcgcgcat gctgcgcatg aatccatcct gctcgaacac ctggctccgc
     1081 gaacggccct gctggcagcc atgctggaat gaggtctaca tgttgaagtt cattgaattc
     1141 gcactgctgg ctgtatcgct gctgattatc ttcggcctgg tggccgtttc tccgcgcacg
     1201 caggcgtctg tcccggagat tgcggagagt acggaggcag aggcgccggt cctgccaaag
     1261 ctgtaagttt ttggtccctg ctaagaacta ccccacccaa gccagaatta tgcctgaagc
     1321 agccaccccg gcaatttggt agcgcactgt gaaaagcaga tacttgccct ttggctcagg
     1381 atgacgaata gatttataga tatggtgagc atcaggctga gagtgccgct ggcttgctaa
     1441 tccgctgacc tgcttccatt cctgataccc tcaaatcagc tatggagaaa aaggaaacgc
     1501 gcgtccggtt tgcgccttca cccaccggac tgctgcacgt gggcaatgcc cgcacggcgc
     1561 tttataactg gctggtggcc cgccatacag gcggaaaatt tgtccttcgc attgaagaca
     1621 ccgacgtgga gcgcagccgc gaagagcacg aaaagcagct catcgaagac ctgcgctggc
     1681 tgggccttga ctgggacgaa ggtccggaaa tgggtggtcc gcatgggccg taccgtcaat
     1741 cggagcggct tgaaatctat cgcaaacata cagagcagct tttaaccgaa ggcaaggcct
     1801 accgctgctt ctgcacggca gaagagctag aagccgagcg ccagaaggcc atcgtggaac
     1861 accgcccgca ggtatattcg ggcaagtgtc gctccattgc gaagaccaat tccgacgggc
     1921 gcgccgctgc cggcgagcca tttgccgtgc gtctacgcat tccggacaaa ccgctgcgct
     1981 ttcatgacat ggtccggggc gaagtggaat ttccgaatga aaccgtgagc gaccctatcc
     2041 ttgtccgttc aagtggcatc cccgtttaca actacgtggt gacggtagat gacgcgctga
     2101 tggagatcac gcatgtcatt cgcggggatg atcacatctc gaacacgccc aaacaagtgg
     2161 ccatttatga ggccttcgga tggcctgtgc ccgagtttgc ccatctctcc acgattctcg
     2221 gcagcgatcg cgagcggctt tccaagcgcc acggcgcgac ttcgattgcg agcttccgcg
     2281 agatgggcat tctgcctgaa gcgctggtga actatcttgc gcttctgggc tggggcgcgg
     2341 agggcggcac gcgcgagacc tttacccgcg aagagctgat caaagaattc aggctggagc
     2401 gtgtgaccgc atcccctgcc gtttttgact tcgataagct gcactggctc aaccggcatt
     2461 atctgaagct gtccagcccg gcgcacgttc tcgatctggc atggccttac tttgcgaaga
     2521 ggcaatggct gcccgcacgc gatgttgcgg acaagcaaac tgtggactgg tttgggaaac
     2581 tgctgacgct ctttctgccc gcggtggacc gcctggagca attgccggag aaggcgcagt
     2641 ttatctttca tttcgatgcg atggctgcaa aggccaacga agaaaatacg cagatcttgc
     2701 aatcagggtc tgcgcagaag gtgcttgggg cctttgcagg gtgcgtccgc gcacatcacg
     2761 gggtagtcac gccggagctt tttaaaagct ggatgaatga agtgaaatcc gaaaccggga
     2821 ggaagggcaa agagttgttt catcccgtgc gcattgctct taccggggcg cattccggcc
     2881 cggagttcga caagctcatc ccgctgtttg aagagggcag tgcactagcc ttgcctgagc
     2941 atgtgctgag cgtgcgcgag cggatagaac aatttatacc ggcatgagtg taaggctgga
     3001 agtgagtttc tcccatagct ggaccgtcaa ggttctcaaa gaacgcccac tcatcgcgcc
     3061 tgcgcggcag ttcgtctatc cgcgacaggc ggaggaagtc gagcgcggtg cgctggagct
     3121 cctcatccag ccagcagacg gagccaaatt cctggccacc tgcgctgcag gttttgctga
     3181 tccctcggcg cctacgggcg tgtggagctg ccctcaccct gactggctct gtgctgtctc
     3241 cggaggctac gcctatctca tccatacgct tcaaccggaa acatggcaaa tggttccgta
     3301 ccgcccggtg ctgggaattc gcgcattgcc tgcacagcaa ctgctgctgt ttatcggaca
     3361 tcattccatc ctcgcctggg gacgggaagg gctggcgtgg cagtcagagc gcctttcatg
     3421 ggagggcgtg caggtcacag gcattgtgga cgatgcactc cacggcctcg gctgggacct
     3481 gatgaccgac aaagatgtgc cgtttgcgtt ggatttgagg acgggaaagc ggattggcta
     3541 aataagcccg tcttcttttt ccgaatggcg gatttgagtg tttggttaat gcgtgtctgc
     3601 cagcctggtc cggtggactt gaagtgctcc agtacctcgg gaaagagcgc aatcggcata
     3661 tatagggcag agccctcaaa gagaggcggc atgaattggc tgcccaggcg ggtagccagc
     3721 gaaatcgtca cgagcaggaa gatcacattg actgcgatgg tgagccagcg gtgacgcaaa
     3781 cagaatttca gaatgggcag ataaatcgcc tgggcgatgc ggaagtaacg atattatgct
     3841 gtatgcctcc taatctccgg cgatggccat ttcctgctct gtctggatgt acttcctgtt
     3901 gcgaagctcc tgttccagct tggcgagctg ggcctcaatg tctttctcct ttatcctgcg
     3961 ctggataatg tcctcgcggt agcggcggag gaaataatct attgtctcca cgcgaatgcg
     4021 gatggaaccg gacggcttgt tctcatcgag atccttgaag gagaaatacg gggtcccgga
     4081 ctcttcgatg atgccttcga tcactccata gatgggtgcg tcgtgcccgc acttgaagct
     4141 tgatacttca agtgcgacga ggtttggatg gcgcgctgta aatttcgcgg cccagacttt
     4201 gtgattggtg ctggtggagt agcggttctt ccacacatcg ctgatatcca acgggtgggt
     4261 gatgacgccc gcccgaactt catcgccgaa gaggcgttcg agcaggtctt catcgagagg
     4321 aagcgtgctc tgcgaaaaga ccggataacc aagcttctgg aactcttcca taatctcgtg
     4381 gttcaggcca ggatcgtgat gatagacgcg tcccagcatg acgatgccga tacggtcttc
     4441 gcgctcaagc tggtcgagca cttcgcgcgc cttacggcga atttcggctt cgtagtcgag
     4501 aagggccgtg aagccaactt caatggcgcg gtcgttttct tcgcgcgaaa ggccaagtat
     4561 cggtgcccag gccttgtaca tctggtcggc gcagatcttc tggtcctgca gattgagaat
     4621 tggatcgaga tacgagatgc cgttctcggc aaagatatcg ttttcttttg tgaatgctgc
     4681 tttcacaact tccggagtgg ctgtcacagt cgggcaggcg ttcgagccgg tcagatttac
     4741 cagcggcgtg tgcaggacgt catacatcgg gaaccagacg gcgttgagcg gcttcttgtt
     4801 gtgcttcgta gtgagcaggt tgtaaacgtg cgcgatgccg atcttcgatg gatagcaggg
     4861 gtcaatcgcg ccgcggctcg cgcctgcgcg gtaaagctcg ggcgtggtgt agtccgaata
     4921 gatgatgttt tcagccttga tgcccagact ggcaaagtaa gcattgaaca gcggcgcgta
     4981 tgtgtagatg ttcagcagac gaggaatgcc gatgcggaag tccttgcggt gttccatcag
     5041 cgcggcgcgc tctttgatgg cctttgagac gaagaggcct tttgcgtttg ggaccggatc
     5101 ggcgacaact tggacctcgc ggtgacggaa aacatcgtgt gctgcaatgt ccacgaagtt
     5161 cgggtttgcg gccttgatct tgtctatgtc cgccttgatg tccttcatct cgtcgaggtc
     5221 ttcgacggtg cctttttcgc aggtggcgat gatgaggcgc tgctcacctt tgcgcagagg
     5281 cactttggtc ttacgcggct ggaattcaac tggtttgggc agctcgacga ctttgctaga
     5341 tccgggcgca agctgaatct gcacgagagg ctcggccttt gctttaaagg gagaggcatg
     5401 ggtgctgcat gacgaaccgt gtgtatgggc ttctgcttca ggcaggtttg cctgaatttg
     5461 ttcaaggctg ggcaggttct ggatatttgt aactgccgcg gttacttctt ccggagtagc
     5521 ggcgcggtcg gtatgctctc cattcacatc cacgtcaata aatgtgcgca ggcagttgtt
     5581 tttgcagaag tagcag
//
LOCUS       DASRBV010000146         8184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_616665, whole genome shotgun sequence.
ACCESSION   DASRBV010000146 DASRBV010000000
VERSION     DASRBV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616665"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1304
                     /locus_tag="VFA02_05510"
     CDS             453..1304
                     /locus_tag="VFA02_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZU09341.1"
                     /translation="MGRRMSSRFVAGMDIEEVIRSARALGNLGIASTLDSLGENVTTP
                     EEARRSAEIYHRLMDAIERQKLNANVSLKLTQMGMDLDPALAEEIVAELVQHAVAANT
                     FVRVDMEGSEYTQATIDMVQRLHAIAGNRGHVGVVIQAYLYRSADDIRTLTGDGIRIR
                     LCKGAYKEPASIAFPEKKDVDANFVKLMRVLLTSGIYHGIATHDEHMIAATKQFVREH
                     GLDPNCFEFQMLYGIRRDLQKSLVREGFHMRVYVPFGAEWYPYFMRRLAERPANALFL
                     ARNLLKK"
     gene            complement(1312..1800)
                     /locus_tag="VFA02_05515"
     CDS             complement(1312..1800)
                     /locus_tag="VFA02_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU09342.1"
                     /translation="MASKLQAEIKQTRPFPSLEDEAVLNIVRTADRLQYHFQQTLKPH
                     GITPTQYNVLRILRGAGENGLRCSDIGERLISSDPDITRLLGRLQKQRLVRRRRNPKD
                     RRVIYTRISPRGLELLEELEPVVNNHAKTLLKGFSKEQLSTLISLLEEIRGPLVQEGP
                     AE"
     gene            complement(1854..2450)
                     /locus_tag="VFA02_05520"
     CDS             complement(1854..2450)
                     /locus_tag="VFA02_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZU09343.1"
                     /translation="MQSVNKNTLFAALLALAASAAHAQATKWQIDPAHSQAEFSIRHM
                     AISNVHGRFGNINGTVVLDEKDIAKSSVNAVIDVTTVDTGVAQRDQHLKSPDFFDVAK
                     YPQMTFTSKGLSNANGQLQLTGDLTIHGVTRPVSLALESPGKEQVDPWGKTRRAFSAT
                     TTLHRQDFGLVWNGNLKSGDSVIGDDVKVELDIELIKQ"
     gene            2577..3833
                     /locus_tag="VFA02_05525"
     CDS             2577..3833
                     /locus_tag="VFA02_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU09344.1"
                     /translation="MQIRIFEHFRDLTAAQRHAFTAAFLGWSLDAFDYFLLVFCISAI
                     AVDFHARASDVAEALFLTLAFRPVGAFLFGALADRFGRRPVLMLNIACYSIFELSCAF
                     APSLHALLVLRALFGIAMGGEWGVGAALAFETLPKEGRGFFSGLLQEGYAVGYLVAAA
                     AYALLYRPLAHISWHGHAVGWRGLFVIGALPALLIFYIGSRVEESPVWSEGRQRRDKV
                     SISRDDIGKYAATFLFLVLLMTGFNLFSHGTQDLYPTFLQKDHGYSAGLTGTIAVVYN
                     VGALLGGVFFGGISERFGRKRAIIGAALLSLPVVPLFAFGRSAEVLAAGAFLMQFMVQ
                     GAWGVVPAYLTELSPAPVRATFPGLAYQLGNLLASRNAVIQAKAAEHYGNYAVVLAAT
                     VVIVALYLTCVTALGREAKGTEMSAS"
     gene            4046..4747
                     /locus_tag="VFA02_05530"
     CDS             4046..4747
                     /locus_tag="VFA02_05530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZU09345.1"
                     /translation="MQRRDFMKGLMAASVGAKAMLAQQPSPAAPSTPPPAPTAPGPVP
                     WMRGLSQIQPNAIRQVVPDAVAGTHARFFSDTQFATLRKLSEILMPPMRGYPGALDAG
                     APEFLDFLIGVSPQEQQQMYQSGLDRLESEAKHRFGKPFASVSAEEADQLIRPWLRTW
                     MSDHPPKDGHERFINVAHSDIRTATVNSREWAEAAKEKGKQPPDQGIFWYPVDPDMRR
                     EGQAACKAAGKKRHL"
     gene            4784..6355
                     /locus_tag="VFA02_05535"
     CDS             4784..6355
                     /locus_tag="VFA02_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZU09346.1"
                     /translation="MAEEKVDVLIIGSGHSGGMAAKILTEKGISCLMLNAGPIPDPRK
                     DTEWKPAYALPYRGFKEPGKVPHIFQANEFNANTWVDEQEVPYTYNPQHPYNWVRVRL
                     FGGRSLFWSRQSFRLSDYEFKAKSHDGYGDDWPISLADVAPYYSRVEEIFRVTGTKEG
                     VPQYPDGNFIPDDSPWTGCMKRFIAAGKKLGVPVYKARQSLGVNGLASSVNLLLPDAF
                     ATGRLKAISNVVVRELGVDKNTGLVNEAHFVERHSRREMSVKARVVVLAAGTLESTRL
                     LLNSKLANSSGVMGHYLIDQVYGPGIVCSVPEARDGKATPELIGGTALVPRFRNIDTK
                     AANFIRGYALNVYSGPGPMDPRNFAAYGEELQRKMDSYHGSGFSTSIMGEVLARYENQ
                     VSIDPHVVDAWGIPVLHIDTKYTDNEFNMARDAVDTSIAMAEAAGFEVLTKNYDPNPP
                     GYSIHELGTCRMGDDPKTSVLNKWNQSHDIKNLFVVDGACFVSGGWQNPTMTILALAM
                     RASEYLAEQMRQGNV"
     gene            6598..6870
                     /locus_tag="VFA02_05540"
     CDS             6598..6870
                     /locus_tag="VFA02_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09347.1"
                     /translation="MQVFCLATAEERADVCCSICGQKYALYFERKSMEERMAAMQMVK
                     ETLDRHHLLGEGKDVHPQKAFTVPEWHGPAHMSGAAILGGAPPWAA"
     gene            complement(7079..>8184)
                     /locus_tag="VFA02_05545"
     CDS             complement(7079..>8184)
                     /locus_tag="VFA02_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09348.1"
                     /translation="ASTYSGIRFLVVLEPLLSPAQTNARVYGPDYVVVTSPVNGSIRM
                     QEVRHTYLHYMIEPLLYSRASAMDRMLPILKTVRDAPLDFQYRADIVSLVIECLIRAV
                     EARTMDTGIAEYKIPPDVRRADLERETQKRNASLQKIAAIRQQAVNKDMVSGFVLTQY
                     FYDQLIAFEHTPDSLKEAIGPMVYGMDVDQQVHRARDIEFAEEGSSDVISHVSRKPHG
                     LDLAEIKLHQGDAAGAQQLAQQALREHTPDPGRANYVLALTSLMQGHMDEAEKSFNET
                     IRLSKDPRLLAWSHIYLGRIHDVRDERDQALVEYQTALSVRDGQPDTKAAAEKGLKQP
                     FELPKRTQPEDDGSGDAPAAQSGAQSPANSPHL"
BASE COUNT         1716 a         1971 c         2517 g         1980 t
ORIGIN      
        1 gtttttcccg ttatgtcgaa gacaaagatc ctgccttcgc gcaggtaatg tagcggcttt
       61 gcgcactcag ggtttgcgca attatttacc ataaccatct ccccgaaaaa ctgctgcgct
      121 catgggctgc ggcggatgag caaaagtgtt gtgaactggc tggagaactc gactctccaa
      181 cagaatactt ttcgctggta ctaagtttga tgccctgcta tgacaggcag gtttctcagc
      241 gggtaaaaat tacgagaaat ttttgctata cgaggggaaa agttactgcc agcccgacat
      301 taaaccggtt ttgtgggcgg gaggtgtgcc cttattgtgt gagagaataa aaagttcgtc
      361 taaaaagcct gaaagatatg ccgattcttc gttctttttt tattgctctt tcccgtaatt
      421 catctataag aaagttttcc gaacggtcag ccatgggccg ccggatgtca tcgcgctttg
      481 ttgccggaat ggacattgag gaggtcatcc gttcagccag ggcgctggga aatctgggca
      541 tcgcttcgac cctggacagt ctgggcgaaa atgtgaccac tccagaggag gcgcggcgtt
      601 cggctgagat atatcaccgg ctgatggatg ccatcgagcg ccagaagctg aatgccaatg
      661 tgagcctgaa gctgacgcag atggggatgg accttgatcc ggcgctggct gaggaaattg
      721 tggcagaact agtgcagcat gcggttgccg ccaatacttt tgtcagggta gacatggagg
      781 gcagcgagta tacgcaggcc accattgata tggtgcagcg cctccatgca attgctggaa
      841 accgcggcca tgttggggtg gtgattcagg cctatcttta tcgaagcgca gacgatatcc
      901 ggacactgac aggagatggc atccgcatcc ggttatgcaa aggcgcctat aaggagccag
      961 ccagtattgc ctttcctgag aagaaggacg tagacgcaaa ttttgtgaag ctgatgcggg
     1021 tactgctcac cagcggtatc tatcatggca tcgccacgca tgatgaacac atgattgctg
     1081 cgacaaagca gtttgtccgg gagcatgggc ttgacccaaa ctgctttgaa tttcagatgc
     1141 tgtatggtat tcgccgcgac ttgcagaaat cgctggtacg ggaaggcttc catatgcgcg
     1201 tgtacgtgcc ctttggggca gagtggtatc cgtactttat gcgacggctg gcggagcgtc
     1261 ctgcaaatgc gttgtttctt gccaggaacc tgttgaagaa ataaccagca cctactctgc
     1321 cgggccttcc tgcacgagcg gtccacgaat ctcttcgagc agggaaatga gtgtcgaaag
     1381 ctgttctttg ctgaagcctt tcagtaacgt ttttgcgtgg ttattcacaa ccggctccag
     1441 ttcttccaga agttccaggc cgcgcggcga gatgcgcgta taaatgacac ggcggtcttt
     1501 gggattacgc cgcctgcgga cgagacgctg tttttgcaag cggccgagga ggcgggtgat
     1561 gtcggggtcg ctgctgatga ggcgttcgcc gatgtcagaa catcgcaggc cattctctcc
     1621 ggcccctctc aaaatgcgta gcacgttgta ttgcgttggc gtaatgccat gcggttttaa
     1681 tgtttgctgg aaatgatact gaagacggtc agcggtgcga acaatgttga gcacggcctc
     1741 atcttcgagg ctagggaatg gacgggtctg cttgatctct gcctggagct tgctggccat
     1801 acatgctttt ataacccaaa tacgggcacg ccggggcgac gcgcccggtg gatttactgt
     1861 ttgatgagtt cgatgtcgag ttcgaccttg acgtcatcgc cgatgacaga atcaccggac
     1921 ttgagattcc cgttccagac cagaccaaag tcctggcggt ggagggtcgt ggttgcagag
     1981 aaggcacggc gtgtttttcc ccagggatca acctgctctt tacctggact ttcgagggcg
     2041 agcgagaccg gccgggtgac gccatggatc gtcaggtctc ccgtcaattg caactggcca
     2101 ttcgcgtttg aaaggccttt gctggtgaag gtcatctgag gatatttggc gacgtcaaag
     2161 aaatcagggc ttttcagatg ctgatcgcgc tgggcgacgc ccgtatccac ggttgtgaca
     2221 tcaatgactg cgtttacgct ggattttgcg atgtcctttt cgtccagtac tacggtcccg
     2281 ttgatattgc cgaagcgtcc atggacgttg gaaatggcca tatgcctgat gctaaattcg
     2341 gcctggctgt gagcggggtc aatctgccat tttgttgcct gggcgtgcgc agcagaggcg
     2401 gccagggcca gcagggcggc gaagagggtg tttttgttta cggattgcat ggaaattatc
     2461 gttctccctg gaaatttgaa gcagtgatgc tgtttagata caggagaatt ttattcgttg
     2521 caacagttat tttgcggaaa aattctgctt ttgttctgct actctcttga tttcgcatgc
     2581 agatacgcat tttcgagcat tttcgtgacc tgactgcggc gcagcggcat gcctttacgg
     2641 cggcttttct gggatggtcg ctcgatgctt tcgactattt cctgctggtc ttttgtattt
     2701 ctgccattgc agtggacttc catgcccgcg cgtcagatgt ggccgaggcg ctgtttctga
     2761 cactggcctt ccgccctgtg ggagcgtttc tctttggcgc attggcagac cggtttggaa
     2821 ggcggccggt gctgatgttg aatatcgcgt gctactcgat ttttgagttg tcctgcgcct
     2881 ttgctccgtc tcttcatgcg ctgctggttt tgcgcgcctt gtttgggatt gcgatgggtg
     2941 gcgaatgggg cgtgggtgct gcgctcgcat tcgagaccct gccgaaggag ggacgtggct
     3001 tcttttccgg tttgctgcag gagggctatg cggttgggta cctggtggcg gcagcggcct
     3061 acgcgctgct gtacagacct ctggcacata taagctggca cggacacgct gtgggttggc
     3121 gcggactctt cgtgattggg gcattgcctg cgctattgat tttttatatc gggtcgcgtg
     3181 tggaagagtc tccagtatgg agcgaaggac ggcaaaggag ggacaaggtt tcgatttccc
     3241 gggacgatat tgggaaatat gcagcgacat ttctattttt agtgctgctc atgacgggat
     3301 ttaacctgtt ctcgcatggc acccaggacc tctatccaac ctttctgcaa aaagaccacg
     3361 gttattcggc cgggctgacg ggcacgattg ctgttgttta taacgttggc gcattgcttg
     3421 gaggcgtctt tttcggaggg atttcagaac ggtttgggcg taaacgcgcg attattggcg
     3481 cggccctgtt gtctttgcct gtggttcctt tgtttgcctt tgggagatcg gccgaagtat
     3541 tggctgcggg cgcatttctg atgcagttca tggtgcaggg cgcgtggggt gtggttcctg
     3601 cttatcttac cgagctttcg cctgctcccg ttcgtgctac ttttcctgga ctggcgtacc
     3661 agcttggaaa tcttttggct tcccgcaatg cggtgatcca ggcgaaagcg gcggagcact
     3721 acgggaacta tgccgtagtg ctggcagcca cggtggtgat tgtggcgctg tatctgacgt
     3781 gcgtgactgc attgggcaga gaagcgaagg gcacggagat gagcgcgtcc tgaaagaggt
     3841 ctgagtgcgt cctcatcgcg tcagggagcg aatggaagcg cttgcaattc agtgtgaata
     3901 cttttaatct catcatccaa agagacaatt cggaaaaact gcgctctgaa aaattacaag
     3961 cggaggcaat ggattgtctg cttaccgggt gatgaaggcc ggtggatcca tctgactcaa
     4021 tattcattct gcaggagagg aatgcatgca gcggcgtgat tttatgaaag ggctgatggc
     4081 tgcctcagta ggcgcaaaag ccatgttggc gcagcagccc agtccggcgg caccttcaac
     4141 gcctccacca gcacctactg cgcccgggcc tgtgccgtgg atgcgtgggt tgtcgcagat
     4201 ccaacccaat gcgatccggc aggttgttcc tgacgctgtt gccggaaccc atgcgcggtt
     4261 tttttctgac acgcagtttg cgacgctacg gaagttgagc gaaattctga tgcctccgat
     4321 gaggggctat ccgggtgcgc tggatgcggg agctccggag tttctggatt tcctgatcgg
     4381 tgtctcaccg caggaacagc agcaaatgta ccagtccggt ctggacaggc tcgaatctga
     4441 agcgaaacac cgtttcggaa aaccatttgc ttctgtcagc gcagaggagg cagaccagct
     4501 tatccggccc tggctgagga cgtggatgag tgatcatccg cccaaggacg gccatgagcg
     4561 gttcatcaat gtggcgcata gcgacatccg cacggcaacg gtgaattcac gggagtgggc
     4621 ggaggctgcg aaggagaaag gcaagcagcc tccggaccag ggaattttct ggtatcccgt
     4681 tgatcctgat atgcgccggg aaggccaggc ggcatgcaag gccgccggca aaaagaggca
     4741 tctctgaagc ggacatcttc gcaggagcat atagaggaaa ttgatggcgg aagaaaaagt
     4801 tgacgttctg attattggct cgggccattc cggcgggatg gcggcgaaga tactgacgga
     4861 gaagggtatt tcctgcctga tgctgaatgc aggtccgatt cctgacccgc gcaaggacac
     4921 ggaatggaaa ccggcctatg ctctgcctta tcgcggattt aaagaaccag gcaaggtccc
     4981 gcacattttt caggccaatg agttcaatgc aaacacctgg gtggacgagc aggaagtccc
     5041 gtatacatac aatccgcagc acccatacaa ctgggtgcgg gtgcggttgt ttggcgggcg
     5101 gtcgctcttc tggtcgcggc agtcgtttcg cctcagcgat tatgagttca aggcgaaatc
     5161 gcatgacggt tatggagacg actggcctat cagccttgcc gatgttgcac cgtattactc
     5221 gcgggtagaa gagatctttc gagtgacggg aaccaaggaa ggtgttccgc aatatcctga
     5281 tggcaacttc attccggatg attcgccgtg gaccggatgc atgaagcggt ttattgcggc
     5341 gggcaagaag ttgggagtgc cggtttataa ggcgaggcag tcgctgggag tgaatggatt
     5401 ggcaagctca gtgaatctgc tgctgcccga cgcttttgcg acgggcaggc tgaaggcgat
     5461 ttccaatgta gtggtacgcg aactgggcgt ggacaagaat actggtctgg tgaatgaggc
     5521 ccattttgtg gagcggcatt caaggcggga aatgtcagtc aaagcgcgtg tagtggtgct
     5581 ggcggcgggg acgctggaga gcacgcgcct gctgctgaat tccaaactgg cgaactcaag
     5641 cggcgtgatg gggcactatc tgatagacca ggtgtatgga ccagggattg tctgctcggt
     5701 gcccgaggcg cgcgatggca aggcgactcc ggagctgatt ggcggaacgg cacttgttcc
     5761 gcgttttcga aatattgata cgaaggccgc gaattttatt cgcggatatg ctctgaacgt
     5821 ctacagcgga cctgggccga tggacccgcg aaattttgcc gcctatggag aagagctgca
     5881 gagaaagatg gacagctacc acggcagtgg cttttccacc tcgattatgg gcgaagtgct
     5941 tgcccgctat gaaaaccagg tcagcattga tcctcatgtg gtggatgcgt gggggattcc
     6001 tgtgctgcat attgacacaa agtacaccga caacgaattc aacatggcgc gcgatgcagt
     6061 ggacacgagt attgccatgg cggaggcggc ggggtttgaa gtcctgacca agaattatga
     6121 tcccaatccg ccggggtaca gcattcacga actcggcacg tgccgcatgg gagacgatcc
     6181 gaagaccagc gttttaaaca aatggaacca gagccacgat atcaagaacc tgtttgtggt
     6241 ggacggagcg tgttttgtca gcggcggctg gcaaaatccc acgatgacga ttctggcgct
     6301 ggcgatgagg gcctcagaat atctggcaga acagatgcgg cagggaaatg tttagggcct
     6361 gatgttttcc tgatagtgcg cataattgtt gctcataaca gcagtttgca gcttgtctat
     6421 ctctggaaat ccatgggatg atgacgcacc ggagaaggca ggatgcgcat aagatttcat
     6481 cattcattca agtgaaaagg agttattaac aaccagcgtc ctccttcaaa tgcatctaaa
     6541 ccaggaaacg atgtgaaacc gtaagacact atctgcttac gagtgaagga ggacagaatg
     6601 caggtttttt gccttgcgac ggctgaggag cgcgctgacg tttgctgcag catctgtggc
     6661 cagaaatacg ccctttactt cgaacgcaaa tccatggaag agcgcatggc cgctatgcag
     6721 atggtgaagg agactttgga tcgtcaccat ctgctcggcg aggggaaaga tgtgcatccc
     6781 caaaaggcat ttaccgtgcc tgagtggcat gggcccgcgc atatgtccgg ggctgcgatt
     6841 cttggcggcg ctccaccgtg ggccgcctga gggcgctaaa gccacctgtt tgcgggattc
     6901 aacggcacgg ctaaaaagcc gtgcccttca aaaccggact ttctgacggc ggggaaaatc
     6961 ccaacaggat ttggctaaag ccttacatcg ctaagattct tcctcccttt ggccgtcaga
     7021 atgacggatt ctgcatatac aaaaaatgct acagcccgcc gcactgggtt tttcgttatc
     7081 agagatgggg tgaatttgcg ggtgactgtg ctccggattg agcagcagga gcatcgccgc
     7141 tgccgtcatc ttctggttga gtgcgttttg gcagttcaaa gggctgcttg agtccttttt
     7201 ctgctgctgc ttttgtgtcg ggctggccgt cacggacgct cagcgctgtc tggtattcca
     7261 ccaaggcctg gtcgcgttcg tcgcgtacat cgtggatgcg gccgaggtaa atgtgggacc
     7321 aggccagcag gcgcgggtct ttcgagaggc gaatggtttc attgaagctc ttttcggcct
     7381 cgtccatatg cccctgcatc aggctggtca gtgcaaggac atagttggcg cggcccgggt
     7441 cgggcgtgtg ctccctgagg gcctgttggg caagctgttg tgctccggcg gcgtcgccct
     7501 gatgcagttt gatttccgcc agatccagtc catgtggctt gcgcgagaca tggctgatca
     7561 catcagacga accttcttct gcaaattcga tatcgcgggc gcggtggacc tgctggtcta
     7621 cgtccattcc atagaccatg gggccgatgg cttccttcag gctgtccgga gtgtgctcga
     7681 aggcaatgag ctggtcatag aaatactgcg tgagcacaaa gccggagacc atgtctttat
     7741 tgacggcctg ctggcggatg gcggcgatct tctgaagcga ggcattgcgc ttttgtgttt
     7801 cacgttcgag atcggcgcgg cgcacgtcgg gcggaatttt gtattcggcg atgcctgtat
     7861 ccattgtgcg cgcttcgaca gcgcggatga ggcattcaat gaccagcgag acaatatcgg
     7921 cgcggtactg gaagtcgagt ggggcatcac gcacggtctt gagaatgggc agcatgcggt
     7981 ccattgctga ggcgcgcgag taaagcagcg gctcaatcat gtagtgcagg taggtatggc
     8041 gcacctcctg catacgaatg gagccgttta cgggcgaggt cactacgacg taatccggcc
     8101 cgtaaacacg ggcgtttgtc tgcgcgggtg aaagcagcgg ctcgaggacg acgagaaagc
     8161 ggatgccgct ataggtgctg gcgg
//
LOCUS       DASRBV010000147         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_671915, whole genome shotgun sequence.
ACCESSION   DASRBV010000147 DASRBV010000000
VERSION     DASRBV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671915"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1658
                     /locus_tag="VFA02_05550"
     CDS             159..1658
                     /locus_tag="VFA02_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09349.1"
                     /translation="MSTITAVPGIQPDGKAPVLPRQSAPRRCDATRYMCASIFLGHDK
                     EIRKNLRAFAADRHHASVPNYGLNVRLLVRVLDFFDRRKFRFALWHGPLGLLIFVVLV
                     VMYATADETTQPMTIFAIVLAAVLLAIPEYRQVMRNRYKYPLRYFSRATFDPERAMEA
                     FRNSHGKLEREIAGREFGGNTTVYGGFEPFTGEGGALGDWSLVIDTRKGKEGESPMKF
                     SEQELEDAIQASFDKLALPGLEIKEHLFVHGCDIGQIQELLPDRFTAPRSDVTEATLS
                     AFRHSDSHDARVYKSLSAWSWNDQVRISFFYRTIFQGPMLYIEAHSRVLHPVAASYRE
                     IDNLTEPTAGKKFSIFIGLAIITPVLCLGESFGLFLRMRDASLLRKLEKVEKKKIEEQ
                     ARYNYGATSSLRESFSALVYDHYFEKSDRELYLTAIEKQLLDSILEFLESKNVDTSDL
                     KEERTYIINSGLIVHGGLQTAALAVGDQAQAMAGKKPRAQKEKTGGKAA"
     gene            1655..2008
                     /locus_tag="VFA02_05555"
     CDS             1655..2008
                     /locus_tag="VFA02_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09350.1"
                     /translation="MTQKSNANYGIQADSVKAGAIAVGPHAHAVATPAATPGAEQENA
                     IRKLKETVQQLSLASTQQKELLDHLDTLSKGPPPQRASTIDKIVSICTQAGHLAAVVA
                     PLKAVAAAFGLVLPF"
     gene            complement(2005..3156)
                     /gene="alr"
                     /locus_tag="VFA02_05560"
     CDS             complement(2005..3156)
                     /gene="alr"
                     /locus_tag="VFA02_05560"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZU09351.1"
                     /translation="MNTRPVLAEISRSALLNNWNILRAAAPADAEVVAVLKANAYGHG
                     VLLCGPVLAKAGAGWFGVTCVEEGIALRKACPEARILVMSGLWEGEEEAVIEHRLTPQ
                     VWEPFHFELLEKAAGGQRVAVHLEIDTGMSRQGVRSLDELKGLLQRYGAGPARIEGVM
                     THFCAPEVLEPDTTRVQIVRFAAALEVLNQHGIRPEFIHGGNSATVLAAQHRGPLAAL
                     AKKYGARLMLRPGIALYGYPPRFAPSSPDRLLVQPVLSWKTRIVSMRDVEAGATAGYN
                     MTFRAERASRLALVPVGYADGLNRMLSNCGQALVRGEKVRIAGRVSMDQTILDVTDVP
                     EASIGDEVVLIGEQGGARITAFDLADATGTIPYEVTCAISARVPRVLVD"
     gene            complement(3277..6762)
                     /locus_tag="VFA02_05565"
     CDS             complement(3277..6762)
                     /locus_tag="VFA02_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HZU09352.1"
                     /translation="MAASAIAISGVAQTMQAPDITKEPTLYVVPYAHLDTQWRWEFPQ
                     VISEYLLKTMRVNFDYFQKYPHYVFNWTGSNRYRLMKEYYPNDYEKMKEYVEKGNWFP
                     AGSSVEEGDVNLPSAESIFRQVLYGNTYFRKEFGKASNEYMLPDCFGFPASLPTILAH
                     AGVKGFSTQKLNSGWQPAPQVGGPDSPEQTPEGIPFNVGLWEGPDGSTVLAALNPGGY
                     GSQIRTDLSKAPPPSPEADPNAPRGFRPPREQDWPKRIDLDGKVTGVFADYHYIGTGD
                     IGGAVDEESAKLLEATVTQGTAVLPGDGSTPVRMGDGPVHVISSAADQMFNDIKPEMQ
                     TKMPRYKGDLELINHSAGSLTSQAYHKRWNRENELLADAAEKASVAAEWMGGRPYPQK
                     RLNDAWTLVMGGQFHDTAAGTATPRAYEFAWNDDVIALNQFAGVLTSATQAIASGLNT
                     QVSAGIPVVVYNPLNIERQDVVEATLHFPGAQPKAVRVTGPDGEEIPAQVETNKVLFL
                     AKVPSVGYAVYNVRPAATATQKHTSELKVTENSLENARYKVELNKDGDVSSVFDKKLK
                     KELLSAPVRLAISTDAPHMWPAWNMDFDQEQAAPRSYVSGPAKVRIVEDGPVRVAIQV
                     SRETEGSKFVQTVSLAAGDAGNRVEFGEAIDWRTLSANLKAVFPLAAGNKMATYNWEV
                     GTIQRPNAYDRQFEVASHHWIDLTDDSGSFGATILTGPKNGSDKRDDHTIRLTLLRSP
                     GYRPDDKRHAYSDQQNQDWGHHEIVFGLAGHAGDWRQGQTDWQGYELSSPLIAFETEK
                     HPGTLGRSFSLLTINNPRIRVLALKKAETSDEYILRMVELDGKQQENVQVKFAGPITE
                     AREVNGQEQPLGPAIVTGGALDVSFKPYEPRTFALKLGTPPAHLDGVQSQPVTLKYDL
                     AAASNDDTRSEGGFDAKGNALPAEMLPTELPFHGVTFQLAPAGTAKPNALVAQGQTIQ
                     LPDGNYDTVYILAASDDGDQKVTFAVGKKPVELNIQNWGGFIGQWDTRLWKPAPDTVT
                     VRQFRNGPEKQVALRKDWAVSANHATWDLSDRGSPDWAPRYPEDYLGLRPGYIKRAEL
                     GWYASHHHTPDGLNEPYQYSYLFAYAIPLPPHATTLKLPANDKVRILAISMAHVGPAV
                     TPAQPLYDTLKQDQQ"
BASE COUNT         1426 a         1985 c         1885 g         1467 t
ORIGIN      
        1 taaaaaaata ttctgtcacg caagccaaaa caggagcaca atatccaaga cagtattccg
       61 gcccagttcg tcgaacaccg gtcgcaacct ttgcctcgtc cttctgctct acgttccatc
      121 cggcgatcgc gatttcaaaa aacctaacgg agggaagtat gagcacgatt actgctgtcc
      181 ccggcattca gcctgacgga aaagcacccg tattgcccag acagagtgca ccacgccgtt
      241 gcgatgcgac acgctatatg tgcgcgagta tcttcctcgg tcacgataag gagattcgca
      301 aaaacctacg cgcattcgct gccgaccggc accatgcctc ggtacccaat tacgggttga
      361 atgttcgtct gctggtcaga gtgcttgatt tcttcgatcg gagaaagttc cgctttgctc
      421 tctggcatgg gcctctgggg ctgcttattt tcgttgtact tgtcgtgatg tacgccactg
      481 ccgatgaaac aacgcagccg atgaccatct ttgcgattgt gcttgccgcc gtcctgctgg
      541 ccattcctga atatcggcag gtcatgcgca accgttacaa atatccgctt cggtatttct
      601 ccagagcaac attcgacccg gaaagggcga tggaagcgtt ccggaacagc cacggcaagc
      661 tggagcgcga gattgccgga cgcgagtttg gcggaaacac gaccgtttat gggggctttg
      721 agccgtttac aggagaaggt ggagcactgg gcgactggtc gcttgtcata gacacgcgaa
      781 aaggcaaaga gggagaatcg ccgatgaagt tcagcgagca ggaactggaa gatgcgattc
      841 aggcgtcttt tgataagctg gcgctccctg gcctggagat caaagagcac ctctttgttc
      901 acggatgcga tattggccag atacaggagc ttctgccgga cagattcaca gctccgcgtt
      961 cagacgttac tgaagccact ctgtccgcat ttcgccattc cgactcgcac gatgcgcgcg
     1021 tctataaatc gctttcagca tggagctgga atgaccaggt gcgtatctcc tttttctacc
     1081 ggaccatctt tcagggcccg atgctctata tcgaagctca ttcgcgcgtt ctgcatcctg
     1141 tggccgccag ttaccgggag attgataatc tcaccgagcc aacggcaggg aaaaagttct
     1201 ctatctttat tggcttagcc atcattacgc ctgtcctctg cctgggtgaa agttttggtc
     1261 tttttctgcg catgcgggac gcgtctcttt tacggaagct cgaaaaagta gagaagaaga
     1321 agatcgaaga gcaggcccgt tataactacg gggccacaag cagccttcgg gagagctttt
     1381 ctgcgctcgt ttacgaccac tactttgaaa aatcagaccg cgagttgtat ctgactgcca
     1441 ttgagaagca gcttctcgac agcattctgg agtttctgga gtcgaagaac gttgatacgt
     1501 ctgatctcaa agaagagcgc acatacatca tcaacagtgg tcttattgtt catggaggac
     1561 tccagaccgc agcgctggct gttggcgacc aggcccaagc catggctgga aagaagccga
     1621 gggcccaaaa agagaaaacg ggaggtaaag ccgcatgacg cagaaatcaa acgccaatta
     1681 cggcattcag gctgacagcg tcaaagcagg ggccatcgcg gtcgggccac acgcccacgc
     1741 agtggccacg cctgccgcca cacccggcgc agaacaggaa aatgcgatca ggaagctcaa
     1801 ggaaactgtt cagcaacttt cgctcgccag cacacagcag aaggaattgc tcgaccatct
     1861 ggatacgctc agcaaaggac cgcctccaca gcgcgcttcg acgatcgaca agatcgtctc
     1921 tatctgcaca caggcaggac atcttgcagc ggtagtggct cctttgaagg cggtggccgc
     1981 tgcgtttgga cttgtgcttc ccttttaatc aaccaaaact cttggcacgc gggcgctgat
     2041 cgcgcaggtc acttcataag gaatcgtgcc tgtggcatcg gcaagatcaa aggccgtgat
     2101 gcgcgcgccg ccctgttcgc cgataagcac aacttcatct ccaatggagg cctcaggaac
     2161 atccgtcaca tccagaattg tctggtccat ggaaacgcgg cctgcaatgc gcactttctc
     2221 cccgcgcacg agagcttgtc cgcaattgga aagcatccgg ttgagaccat cggcatagcc
     2281 tacagggacg agtgccaggc gcgaggccct ctccgcgcgg aaggtcatgt tgtatccggc
     2341 ggtcgcgcct gcctcgacat ctctcataga gacaatgcgc gtcttccatg acaaaacagg
     2401 ctggaccagc aatctatccg gcgatgacgg cgcaaaccgc ggcggatatc cataaagagc
     2461 aataccgggt cgcagcatca atcgcgctcc atattttttt gccagtgctg ccaaaggacc
     2521 gcggtgctgc gctgcaagca ccgtcgccga gttgccgcca tggataaatt ccggcctgat
     2581 gccatgttga ttcagcactt ccagcgccgc tgcgaaacgc acaatttgca cccgcgtcgt
     2641 gtccggttcc aggacctccg gcgcgcagaa gtgcgtcatc acgccttcga tacgggccgg
     2701 gccagcaccg tatcgctgaa gcaatccctt tagctcgtcc agcgaacgca cgccttgccg
     2761 cgacataccc gtgtctatct caagatgcac agcaaccctc tgcccgcccg cggccttttc
     2821 cagcagctca aagtgaaacg gctcccacac ctgtggcgtc aggcggtgct cgatgaccgc
     2881 ctcctcctcg ccttcccaca gcccggacat gaccaagatc cgtgcctccg gacacgcttt
     2941 gcgcagcgca atcccttcct cgacgcaggt gacaccaaac cagcccgcgc ccgctttggc
     3001 aaggacaggc ccgcacagca gcaccccatg gccataggca ttggccttga gtacggccac
     3061 aacctcggca tccgccggag cggccgcgcg cagaatgttc cagttattga ggagggccga
     3121 acgggagatc tccgccagaa cggggcgcgt gttcatcgaa tatgcaaatt cctggaaaca
     3181 aaggattgtg tcatctcacg cccggcaaat ttctgaaata agccaggacc cacgcttcca
     3241 tcatagtgcg ggaagcgtgg gcggaggagg gaagattcac tgctggtcct gcttaagtgt
     3301 gtcgtagagc ggctgggcgg gtgtgaccgc tggtcccaca tgcgccatag agattgccag
     3361 aatgcgcact ttgtcattcg caggcagctt caacgtagtc gcgtgcggag ggagcggaat
     3421 cgcgtatgcg aacagatagc tgtactgata cggctcgttc aaaccgtcag gagtatggtg
     3481 gtgcgaagcg taccagccaa gctcggcgcg cttgatgtag ccgggacgca gaccgaggta
     3541 gtcctcagga tatcgcggcg cccagtccgg cgagccgcga tcgcttaggt cccatgtggc
     3601 gtggttcgcc gagacggccc agtctttgcg aagagccact tgcttctccg ggccattccg
     3661 aaactgacgt accgtcacgg tatccggagc aggtttccac agacgtgtgt cccattgacc
     3721 gatgaatccg ccccagtttt ggatgttcag ctcgactggc ttctttccaa ctgcaaacgt
     3781 caccttctgg tcaccatcgt ctgaagctgc aagaatatac actgtgtcgt aattgccatc
     3841 tggcagctgg atggtctgtc cctgcgcaac caacgcattg ggtttggccg ttcccgcagg
     3901 agcaagctgg aaggtcactc catggaaagg cagctccgtg ggcagcattt cggcaggtag
     3961 agcattgcct tttgcgtcga atccgccttc agatctggtg tcgtcattgc tggcggctgc
     4021 aaggtcatac ttcaaggtga ccggctgcga ctgcacacca tcgaggtgtg ccggaggagt
     4081 acccagcttc agtgcaaagg tgcgcggttc atatggtttg aaggacacat ccagcgcgcc
     4141 accggtcaca atcgcaggac cgagcggctg ttcctgtccg ttcacctctc gggcttcggt
     4201 gattgggccg gcgaatttta cctgtacatt ttcctgctgc ttgccgtcga gttccaccat
     4261 tcgcagaatg tattcatcgc tcgtttcagc cttcttgagc gccagcacgc gaatgcgcgg
     4321 attgttgatg gtcaggagcg agaagctgcg gcccagcgtt cccggatgct tctccgtctc
     4381 aaaagcaatc agcggagagc tgagttcata gccctgccag tcagtctgtc cctgccgcca
     4441 gtcacccgcg tgtccggcca acccgaagac gatttcgtga tggccccagt cctggttttg
     4501 ctggtcagaa taggcgtggc gcttgtcatc cggacggtaa ccgggcgagc gcagcagcgt
     4561 cagacggatg gtgtggtcat cacgcttgtc cgatccgttc ttcgggccag taaggatggt
     4621 cgcgccgaac gagccgctgt cgtcggtcag atcaatccag tggtgcgagg ccacttcaaa
     4681 ctgacgatca tacgcattcg ggcgctggat ggtaccaact tcccagttgt acgtcgccat
     4741 tttgttgccg gcagcgagcg gaaaaaccgc cttcagattg gctgaaagcg tgcgccagtc
     4801 aatcgcttcg ccaaattcca cacgattgcc tgcgtctccg gcagcgaggc ttacggtctg
     4861 cacgaacttc gagccttctg tttcgcgcga aacttgtatc gccacgcgca ccgggccgtc
     4921 ctccacaatg cggacctttg ccggaccgct gacataggag cgcggagcag cctgctcctg
     4981 gtcgaagtcc atgttccacg caggccacat gtgcggcgca tcggtggaga tggcaaggcg
     5041 caccggcgcg gagagcagct ctttcttcag cttcttgtca aagacgctgc ttacatcgcc
     5101 gtctttgttc aactcaactt tgtaacgtgc attctcaagt gagttttctg tgaccttgag
     5161 ttcggaagtg tgtttttgag ttgcagtagc agcaggacgc acgttataga ccgcataccc
     5221 caccgacggt actttggcca ggaacagtac cttattggtt tcgacctgtg cgggaatttc
     5281 ctcgccatca ggcccggtca cgcggacggc cttcggctgc gctcctggaa aatgcagtgt
     5341 ggcctccact acatcctgac gttcgatatt cagcggattg tagaccacta ccggaatccc
     5401 cgcagatacc tgcgtattca gtcccgaagc aatggcctgc gtcgcactgg taagcacgcc
     5461 cgcaaactga ttgagcgcaa tcacatcgtc gttccaggca aattcatagg cgcgcggggt
     5521 tgcggtgcct gcggcggtgt catggaactg gccgcccatc accagcgtcc aggcatcatt
     5581 cagacgcttc tggggataag gccggccgcc catccattcg gcagcgactg aggccttttc
     5641 tgccgcgtcg gccagcagtt cgttctcgcg gttccagcgc ttgtgatagg cctgcgaggt
     5701 gagtgatccc gcggaatgat tgatgagttc gagatctccc ttgtagcgcg gcatcttcgt
     5761 ctgcatctcc ggcttgatgt cgttaaacat ctggtctgcc gccgaggaga tgacatgcac
     5821 cggtccatcg cccatgcgca ccggagtcga accatctccg ggcagaacag ccgtgccttg
     5881 cgtcaccgtc gcttcgagca gcttcgccga ttcttcatcc accgcgccgc cgatatcacc
     5941 tgtcccgatg tagtggtagt cagcgaagac gccagtgact ttgccatcca gatcaatccg
     6001 cttcggccag tcctgctctc gtggaggccg gaagccgcgc ggcgcattcg ggtccgcttc
     6061 cggagaaggc ggtggggcct tgctcaggtc agtgcgtatc tggctgccat atccaccggg
     6121 attgagcgcg gccagtaccg tcgagccatc cggcccttcc cacaacccga cattgaacgg
     6181 aattccctcc ggcgtctgct ccggagaatc cggcccgcct acttgcgggg ccggctgcca
     6241 gccagaattc agcttctgtg tggagaagcc ctttacaccc gcgtgcgcaa gaatcgtggg
     6301 cagcgaggcc ggaaacccga agcagtcggg caacatgtat tcattgctgg ccttgccgaa
     6361 ttccttgcga aaatacgtgt tcccatagag cacctgtcgg aagatggatt ctgcgctggg
     6421 cagatttacg tcaccttctt caaccgaaga accagccgga aaccaatttc ccttctcgac
     6481 gtactccttc atcttttcgt agtcgttcgg gtagtactcc ttcatcaggc gataacggtt
     6541 tgatccggtc cagttgaaga cgtagtgggg atacttctgg aaatagtcga agttcacacg
     6601 catggtcttc agcagatact cgctgatgac ctgcggaaac tcccagcgcc attgcgtatc
     6661 gaggtgcgca tagggcacga cgtaaagtgt cggttctttg gtgatatcag gtgcctgcat
     6721 ggtctgtgca acgccgctga tggcaatggc agaggcggcg atg
//
LOCUS       DASRBV010000148        25358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_59812,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000148 DASRBV010000000
VERSION     DASRBV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59812"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..617)
                     /locus_tag="VFA02_05570"
     CDS             complement(33..617)
                     /locus_tag="VFA02_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09353.1"
                     /translation="MTKRKWAVFVVIILVFLAVFWLVAGRHQTPPGQPPLADINQQSL
                     AQLRQEFNASSDSERVLLLLSPTCPVCVAGTSEVNAVLRRHPRSHVRVFAVWEPILPT
                     DWSRPTSVVLHRLSDTRITQMWDKQHLVADLVKNSTDGLDPGCCKHDGVLWDIIAVYP
                     PGAQWTDSLPAPELFAGPVVRGAPQWNTKLLQAN"
     gene            complement(633..965)
                     /locus_tag="VFA02_05575"
     CDS             complement(633..965)
                     /locus_tag="VFA02_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09354.1"
                     /translation="MKNIRTSAALTPVAAILSALATLTCCLPWGIGAALSALGLSVFF
                     ARFQIWFLILSVALLFLGLFQMLRKRQNCQRHSRTEIVLLSVAALVVIAIALFPDWVA
                     GLFVGHLP"
     gene            complement(969..1190)
                     /locus_tag="VFA02_05580"
     CDS             complement(969..1190)
                     /locus_tag="VFA02_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09355.1"
                     /translation="METPITDLDQAQMHGFPGSPTLRVNGQDIEDIPPERLSVGFACR
                     TYLVNGKPQGVPPRSWLEQAIRAARTKEE"
     gene            complement(1289..1726)
                     /locus_tag="VFA02_05585"
     CDS             complement(1289..1726)
                     /locus_tag="VFA02_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HZU09356.1"
                     /translation="MNGLFQIGDVAAQTGLTVDAVRFYERERLLRTAARSSGGFRLFS
                     KSDVEDVAFIRNAQELGFSLQEIRELISLKQTLHPDCKQVEQLLKHKIATVRGKIASL
                     RKLERELQRAMANCESNLRRASDGEADDCPVLMDISHAKRRKK"
     gene            complement(2022..2630)
                     /locus_tag="VFA02_05590"
     CDS             complement(2022..2630)
                     /locus_tag="VFA02_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZU09357.1"
                     /translation="MSSKTALKEVPSTDKIDRITPHWRQSAKEVQKFGTVIRDLPLGI
                     SEDVRREITGRLNVLLADTASLRDLYKKAHWHVSGPTFYQLHLLFDKHFEQQVELVDL
                     LAERIQILGGVSIAMAHDIAETTRLERPPKGREEVPVLISRLLEAHKIILEDARKLAE
                     KASDLGDEGTNDLLISDVVRTNEMQVWFLSEHLVEMPLVFAK"
     gene            3006..4097
                     /locus_tag="VFA02_05595"
     CDS             3006..4097
                     /locus_tag="VFA02_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09358.1"
                     /translation="MERKGTFILGTLFVASLLTACSKPAQSAGAGAQAGGAQAMPVQT
                     EVVAPQPVPRADEYVSTIKSRRSATINPRVSGNLISISVRSGDRVKAGQVMMVIDPVQ
                     QEATVQSLKATEQQKLAVYQYNQVEVERQRKLYQEGVTSRDALDQAEQSYKNSKADYE
                     AAVNSRQAGEKELGFYTIRAPYDGIVGDIPVHVGDYVSSSTLLTTVDENKDLEAYIYI
                     PTERAHEVHMGLPVVIEDNSGREVERTAIDFVSPQVDDQLQGILAKAPVHSPQLRTEQ
                     LVKARVIWSTAPAPTVPVLAVTRIGGQTFVYVAQDQGGKYFAKQRAVKLGDTIGNSYA
                     VEGGLENGDKVIVSGIQFLVDGAPVHPLG"
     gene            4271..7477
                     /locus_tag="VFA02_05600"
     CDS             4271..7477
                     /locus_tag="VFA02_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZU09359.1"
                     /translation="MVDFFIHRPVFATVCALLIILAGAAVIPTLPISLYPQLAPPQVT
                     VTSNYVGANAQVVESAVTIPLEQQINGVEGMHYMTSTSSNDGTSSINVTFNTGYDLNI
                     AAVDVQNRVNTAQGRLPQEIKNTGITITKANPNFVFAAGFYSPDGSLSNQYISNYLDV
                     YVKDPLKRIKGVGDVVIFGERKYAMRIWLDPAKLAARNLTATDVINAIQEQNIEIPSG
                     QLGRPPAPSTQDFQITLRVVGRLSDPKEFEQIILKSTPNGLVQLKDVGHAELGAETYD
                     SNLEYSGHEAIGIGVQQLSDANALDVDKAAKAALVELSKSFPPGMKYVIAFDTTTVIG
                     DSIKEVISTLEEAIVIVIAVIFLFLLDWRATVIPAITIPVSLVGTFAFIKAFGFSINS
                     LTMFGIILATGLVVDDAIVVIENVQRHISLEHCDPHKATSDAMGEVTSAVIATSLVLI
                     SVFIPVSFFPGTTGILYKQFAMTIAFSIAISAFNALTLSPALAALFLRGEEEKFRMLD
                     WTRIRFLSEGYAAFVRAIDGAIHWLGRTYARIIHVTLKLRYVVLLLFFVGLAATIYMY
                     RTVPTGFIPQEDQNYLICVVQTPPGASLNYTTELSHRAEQLILQDPDVFGTFAVPGFS
                     LSGGSAPNYGLIFVPLKPMDERRGKGHSASDIAQRLSPKLFGVPGGIIAVFEPPAVQG
                     IGSFGGFEFQLQDMGRNTLQDLDMVAHKIVAASRQRKDLTGLFTSYTANDPQLLIQID
                     REKAKAMQVPISEVTNALNVYMGSEYINDFDFNNRSYRVYVQADQPFRMKQSDLRQFY
                     VRANNGSMVPLDNIVNISQTSGPQVIYHFNMFRSAEIDGSAAPGYSSEQGLKAMEELF
                     EQNKLQGMTYSWSGLALEEVESQGKAIIIFGLGIIVVYLTLAAQYESFALPFIIMLSV
                     PMAVLGALGLISVRGFPNDVYVQIGLLMLIGLAAKNAILIVEFAEQLREHGRSIMDAA
                     IEAAELRLRPILMTSIAFILGVLPLYFATGAGAKGRNSVGTVIVGGMVVSTFLNLIFI
                     PVLYVLLSTLLSKMKRQRRRPEGCDEQEVGEESAALSS"
     gene            7671..7901
                     /gene="rpsP"
                     /locus_tag="VFA02_05605"
     CDS             7671..7901
                     /gene="rpsP"
                     /locus_tag="VFA02_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZU09360.1"
                     /translation="MIRLARFGARKQPYYRVVVIEKDRARNGRSVEVVGTYNPRTNPA
                     TVNLKRERIDYWTSKGAQLSDTVQRLLKTAVA"
     gene            8052..8339
                     /locus_tag="VFA02_05610"
     CDS             8052..8339
                     /locus_tag="VFA02_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZU09361.1"
                     /translation="MTQVQKPDDMQKLVTEIAKALVDEPGAVVVEAVEKEESTVLRLR
                     VAPSDVGKVIGKQGRTARSMRTILGAVSMKHHHRYTLDILEEHKQVATPGE"
     gene            8366..8923
                     /gene="rimM"
                     /locus_tag="VFA02_05615"
     CDS             8366..8923
                     /gene="rimM"
                     /locus_tag="VFA02_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZU09362.1"
                     /translation="MNNVPQWIVIAHLVRPQGRRGEVLADILTDFPERFSDRRELMLL
                     DAGGRPLRAVSLEAHWLHKGRVVLKFAGCDSISDAEALRGLDVAISREQRVPLDADSV
                     YISDLIGCCVVLPDGAEAGKIVAVDRETTDAPLLVLETGRSDQVLVPFAKSYLRQINL
                     ETRQIVMDLPEGLLDVNSSSSAEEN"
     gene            complement(9039..10604)
                     /locus_tag="VFA02_05620"
     CDS             complement(9039..10604)
                     /locus_tag="VFA02_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09363.1"
                     /translation="MALLQKAAAQERLLVKTISEHPPLVQTYIQHMQPDPQLGSVPYS
                     DEYLLGRVDFSGVFNENLFAQKGQKKRMFSGSKGFLGMLTGMFRLRFSANGFMDMMFV
                     DASSFDQNHYSFAFVRNEFLGEVKTSVFDVQPKPHAGMGRFIGRVWIDDESGNIVRFT
                     GTFSGGNKRNLYFHFDSWRANVQPDIWLPVAIYVQDLDFRGQTYIWGYGLKLPSRESE
                     NESVIVENATDASENTQDVNPLQATRQWQNQSEQNVLERLTRAGLLAPPSDFDHVLET
                     VTNNIIIGNKLNLPEPVHCRVMLTSTLESVAIGNTIVLSKGLIDVLPYEEDLAAVLSF
                     QLAHIVLGHGIDTRYAFNDRLLFPDESTFQRIAMNHSLEQNEEAAKKAVELFKGSVYG
                     DKSGNVGLFLAQLVNMQQRIPSLLMPRLGDSELRSDGQPWLAALQQGAPALQVNNPQQ
                     IAALPLGSHLRIDAWDDRVYQLHAPAAVIVSASDKMPFQVTPIYYRLTRYRPPQSTST
                     PEQTQTQAPAPGH"
     gene            complement(10850..11353)
                     /locus_tag="VFA02_05625"
     CDS             complement(10850..11353)
                     /locus_tag="VFA02_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09364.1"
                     /translation="MKAAHNVVLGLILISGAALKCSAQEPQSPHVAASHAELASASSD
                     NKVPAALLPSAPSSATVAPTPSPLQVPRVANKKYFLLNGFQLGMAVFDVEMTQLCIRN
                     HRCREANPLMPSSHVGQLSVNFALVAYSSGVSYWLKKRRSKVWWLPPCAGIVVHSIGV
                     ATGFEHQ"
     gene            complement(11448..12263)
                     /locus_tag="VFA02_05630"
     CDS             complement(11448..12263)
                     /locus_tag="VFA02_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZU09365.1"
                     /translation="MSTVEILHEPSEISRTEVVNVLGIPITNVTLDEAISRVAVFLSE
                     PYLHHIVVVNTNKFWLADHHPGLGRIIRNAEMVITEYGPVWASRILGTPLKANVRGIG
                     LFRSLLPWLQEWREPVYFFGARQEVLNRMLQQVSACYPHLQIAGARNGFFQSHEEDSI
                     ISSINQSGASVLFVAMGSPRQELFIENHREELAPRVAMGVGGSFDVLAGLKRDAPSWT
                     HHGMEWIYRLWQDPRNLLGRYARVHSWFVYHTLHEKLAHSYGHRTTLHPGGHH"
     gene            complement(12315..14258)
                     /locus_tag="VFA02_05635"
     CDS             complement(12315..14258)
                     /locus_tag="VFA02_05635"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZU09366.1"
                     /translation="MRPLRAILPLVRRQPSGALVREAAWRVWKPYRSSRLRAAIRRGD
                     AKVRFRDLPYYQPRLEQIAPESAKAITDFANLICSGRFPFPGYQTIFFGLTPQWNRDF
                     VSGFEWEQLPAAQLQPVVRHNGSDVKAPWELSRLQFLPVLAKAYLLSGEKQYREIAKD
                     LLSDWLAKNPVGMGVNWTLAMESALRGMSLCFTLALLQPLRPEEEDWGRKVTQAIWEH
                     LLYTESCLEFSHLVRSNHYLGNIAGLLCMAMFLDGPGMERRRHLYLSRIQNEILRQVY
                     EDGGDYEASLGYHVLVLQMFTSAYLLMRAANLKPEQQFAERLQQMYRYLAEIAGPSAT
                     LPHVGDSDDGRVELLTSDLKQMTSLAPGERNSLFVPGMIGMGDGLFGLEYDGDPSDAA
                     WYGLSPVKKRVSFSQKTIFPQSGIGVVRRDNAEVVFCVIPNGIHGGGSHTHNDKLSVI
                     FRLYGKELFSDSGTFCYTRDTARRNQYRSTASHNTVVIDGEEQNTINLDPHYVFCIGN
                     EASVSAIEVTDNADAIAMCASHSGYERLGVEHRRTVRLSNGALRVQDDLLGEGEHSVD
                     LRWIIPSPWQVTAQDNGRFLLRGPGNASMQITSLLPLKWAETPIQISRTYGGATVKGT
                     LVQASGRGPFPCSLVTETHWSQS"
     gene            complement(14605..16560)
                     /gene="asnB"
                     /locus_tag="VFA02_05640"
     CDS             complement(14605..16560)
                     /gene="asnB"
                     /locus_tag="VFA02_05640"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZU09367.1"
                     /translation="MCGIAGMAGTRNADLLDKMLEITRHRGPDDWGTYLSSGNAPDQS
                     VAIGNNRLKILDLSSAGHQPMASPDGNIWVVYNGELFNFHELREELLADGCVFRSHAD
                     TEVLPYLWLKYGPEMVQHLNGMFAFAIWDTRDHTLFIARDRMGVKPLYYTLQGSKLYF
                     ASEIKAILLAPDICPELDIASLRSFLSLLYVPHPDTMFRNIRKLQPGHSLIWKKGKIQ
                     IERYWDNSKPAGLLPGNERDLTQQCREVLKDAVRRQLVSDVPVGFFLSGGLDSSTLLA
                     CAAEVHSGPLRCYTIAFREEHARLEQCGEDPFYARKVAQHFGAECHEIVVDPDVTSLL
                     PKAIWHLDDAIADHAAIATWLICKEARANVTVLLSGQGGDELFAGYRVHLVEKFPSWI
                     RTLPRPVREKFLLAALQTISKNTSRVPGLQPGLVMAYCRYLTRVLKLIDLPPAGQFIG
                     MRSYLRTDELQELLNPDIFAISKPYEEILQEHFDEAASLDFLEQMLYVDQQTFLPDLN
                     LAYSDKLSMAASIEARVPFLDNAVVDFMRRVPSHLKLHGHIQKYILKKAMEGVLPRDV
                     IYRRKAGFSLPVRSWLRSELSEMLGDVLSEKRVRERGLFRPGRVAKMIEDNRTGQRDY
                     TLQLWSLLTLELWQQTFIDASRPCLTA"
     gene            complement(16612..17787)
                     /gene="wecB"
                     /locus_tag="VFA02_05645"
     CDS             complement(16612..17787)
                     /gene="wecB"
                     /locus_tag="VFA02_05645"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZU09368.1"
                     /translation="MPDASLRIMNIVGARPNMMKIAPLIAEMRRYPEIGPVLVHTGQH
                     YDYSMSQVFFDQLRMPDPDYNLEVGSGTHHVQTAEIIRRFGDLVQQDRPNIVLVVGDV
                     NSTIGCALVAAKEKIPVVHVEAGLRSFDRTMPEEINRILTDSISDLLFTTEESGNQNL
                     AHEGIPAEKVHFVGNLMIDSLVGALELAHKSALRGELGLVPRQYAVLTLHRPSNVDNK
                     QQLSSTLDAIAQVAKRVPVIFPVHPRTAVKISEAGLDCVKTWNGGRIPEHGLWMIPPA
                     SYLDFLGLVEQAAMVITDSGGIQEETTYLGIPCLTYRNNTERPVTVLLGTNKLIGADP
                     HRLLRAAMEVLDKLGTNGHRPAHPPLWDGKTAPRIVRVLKEYWQNHKTTIPEAMALS"
     gene            18024..19973
                     /gene="asnB"
                     /locus_tag="VFA02_05650"
     CDS             18024..19973
                     /gene="asnB"
                     /locus_tag="VFA02_05650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZU09369.1"
                     /translation="MCGISGVLAFQNSRFNVTEEFVLRMRDTMTHRGPDGGGLWISPD
                     RKVGLGHRRLSIIDLSNAASQPMSNADGSLQVVFNGEIYNHAEIRQELVQLGHRHWKT
                     DHSDTEVILHAFEQWGIQCLERFRGMFAIGLWDSNAKRLWLVRDRIGIKPLYYSLHHG
                     RITFASEIKALLQDPDQVRAVNEEALFHYLSFVTTPPPGTLFEGICKLPPGSFLTVDA
                     SGQYRVERYWDVLDHTSPLTNVPEEEIAERLITELRTSVALRTLSDVPVGVFLSGGID
                     SSTNAALFSECASGRVKTFSVGYDKNYRGCESELPLAKKFADSIGAEHYGRVLTQDDF
                     LEFLPRMVHLQDEPIADPVCMPVYYVSKLARDNGVIVAQVGEGSDELFWGYGYWRQMF
                     DLVRWNSYPVPRAAKKLGLAGLRMLGKAQSPQYDFLWRATQGLPVFWSGFEGFTHNAK
                     IEVLSPRLRERFRGRTSWEAIRPTYERFQQKCWEIAPVKWMTYADLNLRLPELLLMRV
                     DKMGMGVSLEARVPFLDHKFVELAMSIPESIITKGRQSKHILKKTVRGIVPDEIIDRK
                     KQGFGVPIYEWFFEQFGAFARRELTEFCEKTDFLDPASVMKLVEEGRGWHAWFLLNFA
                     LWWNEFIDARGPGFASAEEPLAVAR"
     gene            complement(19984..20223)
                     /locus_tag="VFA02_05655"
     CDS             complement(19984..20223)
                     /locus_tag="VFA02_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU09370.1"
                     /translation="MNNLIEKLTDIFREVFDDDSIVLRNELTADDVENWDSLTHINLI
                     VAIEKEFKIRFTTGEVSGLKNVGDLISLVSRKISA"
     gene            complement(20226..22007)
                     /locus_tag="VFA02_05660"
     CDS             complement(20226..22007)
                     /locus_tag="VFA02_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HZU09371.1"
                     /translation="MQTDNLPDLDYRRLLAASRKLSAASPKPALKLALLSDAATQQFV
                     PLLKAILDRNGFSAEIYEGAFDAVELEAYNPSSRFYAFGPDVVVILNATQALRASFAK
                     RNGSAASFTDGFLEKTQSIWNAIQSHCNATIIQSNFALPLEKYFGNYDLKAADSFYSA
                     VHALNTGLTQKARGQNGVLLLDIEGVASYIGRKHFFDDRFWDMWKTFCSFDALPYVAQ
                     NLVDILMAMRGRVVKCMVLDLDNTLWGGVIGDDGLEGIKLSAHGEGEAWHRFQCFLKE
                     LKQRGILLAVCSKNDEQNALLPFQSHPEMVLKKEDITIFVANWNDKAENIRAIQQALN
                     IGFDSMVFVDDNPFERNMVRELVPGIIVPELPDDPSEYVRFLCELNLFETTSFSSEDL
                     QRAEMYKQEASRRAVAASYASVEEFLQSLDMRIVVSRFDSFHLPRIAQLIQRSNQFNL
                     TTRRRSEAECERLMHDPDMFPLYVKLSDKLGDHGLISVVILKPDNECLEITDWLMSCR
                     VLSRGVEQYVMNEVFAIAAKNGLKHVAGTYIPTAKNGMVRDFFRSFGFLQTADTPEAT
                     RWILAVDSYVPRNTYIKSCEPEAEAVA"
     gene            complement(22101..22907)
                     /locus_tag="VFA02_05665"
     CDS             complement(22101..22907)
                     /locus_tag="VFA02_05665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZU09372.1"
                     /translation="MTSPVMDASAAVSSQVSPVALYFDLLKATLTNTLFQPEPDADDE
                     NQLRYVNAFIRHYMEGTAISMCPLSRFQNVQYCIEDVLRHGVPGDLIETGVWRGGMTI
                     FMRAVLKAYGVEDRTVWVADSFEGLPAPDADKCPLEAQVHNGKVMKTVYNHFAVSMEE
                     VQRNFQAFGLLDDRVRFLKGWFCDTLPSAPISKLAIVRLDGDYYDSTRDSLTYLYPKL
                     SIGGYVIIDDYGEDTWTYCRRAVDEFRQEHGIKDLLVRVDSKCYFWQRTQ"
     gene            complement(22960..23973)
                     /locus_tag="VFA02_05670"
     CDS             complement(22960..23973)
                     /locus_tag="VFA02_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09373.1"
                     /translation="MYPVDAQETVTQAQSSAQTLLAGTDGRQLRGVWLRLIAFFLPVV
                     LVVAPIIYWVDPFSFFAKPSPVPEHTRFGYAAPLDQALWDVLTYNRGPKPNILVGDSQ
                     TDRLPAETISQITGEPYANLATGGGSLREAISMFWFAAQRTKLRRVYFGVDFMEYSAY
                     SSDRVPGAVAILHNPLLYFMNSTVLEAGLYDISDAVFHHKTNLGPTVNKAAFWQSQLQ
                     YVKVKINRDAYLGSLTEDLKKVVAYCQQNHISFVFIIPPQHADVRRKINELGLDGQYQ
                     NFKQSLEKLAPVYDCDVDNEFTLNEENYNDPFHLTDEAARKAARDIWSGNSQWCRILS
                     EKQ"
     gene            complement(23980..>25358)
                     /locus_tag="VFA02_05675"
     CDS             complement(23980..>25358)
                     /locus_tag="VFA02_05675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZU09374.1"
                     /translation="CPARLRPALLAIVSILFYAVSSAISAGVLLIAALAVFAVARYMD
                     QMDTASLQRRLIFTVALVVLLAYLLFIKLLPDMLAAGTVPSSFTHTFLALGVSYYTFK
                     LMGYLIDVYWRKYPAWSSPVEFVAFASFFPQLPAGPIQRANEFKLPRSSIETSALMRA
                     GLRRILLGIVKKTVLADQLASIISYIDGMQPHYSNMLWFAAILYALELYFDFAALTDI
                     AVGTAGLFGIRSPENFAHPYFAPSISQFWRRWHMSLTFWLTDYVFTPLRMATRNLGNW
                     GLALSITANMVLIGLWHGINIGFLLFGVIQSIYLMVDSFTSAARKRFYRKHRWADVLT
                     NLIGPFFVFLMIAFTLVFFRAGSFVNISYELRHLWDGLESPVASVTRVYYGFGRMHFG
                     LTGIAVTAALVLELCSYLRAQQSRVVAFLPSFSSLPLAMRWAVYYAALATVITLHQQS
                     THFIYVQF"
BASE COUNT         6135 a         6482 c         6727 g         6014 t
ORIGIN      
        1 gccgagacca tgcttcgccg caacgagtct actcaatttg cctgcagaag tttcgtgttc
       61 cactgcggcg ctccacgaac aaccggaccc gcgaacaatt ccggtgcggg aagcgaatcc
      121 gtccattgtg ctcctggagg atagacagca atgatgtccc acaggactcc atcgtgtttg
      181 cagcagcccg gatccaggcc gtccgtgctg tttttgacta agtcagcaac cagatgttgt
      241 ttgtcccaca tctgcgtgat gcgagtgtcg gaaagcctgt gcaggacgac actcgtcggt
      301 ctgctccaat cggtaggaag aattggctcc catacagcga aaacacggac gtggctgcga
      361 ggatgacgcc tcaacactgc gtttacctca gaagtgcccg ccacacaaac agggcacgtg
      421 ggagatagaa ggagaagtac ccgttcgctg tccgatgaag cgttgaactc ctgcctgagt
      481 tgcgctaacg attgttggtt tatgtcggca agcggaggtt gtccaggcgg ggtctgatgg
      541 cgaccggcaa ccaaccagaa gaccgccaga aacaccaaga taatgaccac gaatacagcc
      601 catttgcgtt tcgtcataca ccgccccaaa atttatggaa gatgacccac gaacaagcca
      661 gcgacccagt ccggaaatag agcaatggcg atgacgacca gggccgcgac gctcaacaag
      721 acgatctctg tccggctgtg tcgctggcag ttctgccttt tgcgtagcat ctgaaaaagc
      781 ccgagaaaaa gcagcgccac cgacaaaatc aaaaaccata tctggaacct ggcgaagaaa
      841 acactcaggc ctaatgcact aagggccgct ccgatgcccc agggtaaaca gcaggtgagc
      901 gttgccagag cgctcaggat agcggccacg ggagtgagtg ctgcggatgt tcgtatgttc
      961 ttcatggcct attcctcctt agtgcgcgcg gcgcgaatcg cctgttccaa ccacgagcgc
     1021 ggcgggacgc cctgaggttt tccgttcacc aggtaagtgc ggcaagcgaa gcccactgat
     1081 aatcgctctg gaggaatgtc ttcaatgtcc tgtccattga ctcgcagcgt gggcgaccct
     1141 gggaagccgt gcatctgtgc ctggtccaga tcagtaatag gagtttccat cagttccatc
     1201 ctcaatcctt ctgtttcgag gacatcccgc acaagttgca ccgccgcgct atgattcgga
     1261 caaccaggca aaaacaacag ctccagcgtc atttcttcct cctttttgca tgagaaatat
     1321 ccatgaggac agggcaatca tctgcctcgc cgtccgatgc tctacgcagg ttcgactcac
     1381 aatttgccat cgcccgctgc aattcgcgct ccagcttgcg cagggaggcg atctttccgc
     1441 gcacagttgc tatcttgtgt ttcagcagtt gttccacctg tttgcaatcg ggatgcaagg
     1501 tctgcttcag gcttatcagc tcgcgaatct cctgaagcga aaacccaagc tcctgagcat
     1561 tgcggatgaa tgccacgtct tcaacatccg attttgagaa cagccgaaag cctccgctgg
     1621 aacgcgcagc ggtccgcaat agccgctccc gttcatagaa gcggaccgca tctacagtca
     1681 gtcccgtttg tgcagccaca tcgccgattt ggaagagacc gttcataaac ccatctcatt
     1741 tcgcagatta cactctggag ttaactccag agtcaaggcg tttttgatgc agcttcaagg
     1801 accttgctga taccaaaggg tggcctcgtt tggctctatg cgcgaacgtt tagggatgtc
     1861 ctgttttcat gaccggaaga gccatttggg tgctgcgttg ccgggattcc agatagaaat
     1921 ttttatgaag aacgtttccg tgcaggcgcg cttcgaactt cacctctgaa taaaaaaggc
     1981 cagacatcat gtccggccct ttatgctgag ctaagaacgt tttacttcgc gaagaccagc
     2041 ggcatttcca ccaggtgctc gctcaggaac cacacctgca tttcgttggt gcgaacgaca
     2101 tcgctgatca gcagatcatt cgtcccttca tcgcccagat cggaagcctt ctccgccagc
     2161 ttccgcgcat cttccaaaat aatcttgtgc gcttccagca ggcgtgaaat cagcactggg
     2221 acctcttcgc gtccctttgg tggacgctcc aggcgggtcg tttcagcaat atcgtgcgcc
     2281 atggctatgc tgactccgcc gaggatctga atgcgctcag ccagaaggtc cacgagttct
     2341 acctgttgct cgaagtgctt gtcgaagaga agatgaagct gataaaaagt cggcccgctg
     2401 acgtgccagt gtgctttctt gtagaggtcg cgcaggctgg cggtatcggc aagaaggacg
     2461 ttgaggcgtc cggtgatttc gcggcgcaca tcttcgctga tgccaagggg cagatcacga
     2521 attacggtac caaacttttg cacctcttta gcagattggc gccaatgtgg ggtgatgcgg
     2581 tcaattttat cggtggacgg aacttctttc agggcagtct tgctactcat aattcgattc
     2641 ctccggtttt cctgtttaga tgctttcttc ggggaatctg ccagatattc ttatgacctg
     2701 ggacggggta tttacagaat tcttactttg ggaaacttcc gaccgcttga ctcaggtttt
     2761 cattcagaat gaagaattta caaaaatttg cagaaccagc cactggaacc atgcctgctt
     2821 gaaattgttt gaagcctgag ggagtctgcc ttcatccaag aaagcaatcc aggcgaagaa
     2881 aatttgaaat cacgataatc gtttcaagtt ttgttccggg aagagtagat tggtattcca
     2941 gagtttatat ttgagtaacc ccattggaaa agacttcaat tcgaggaaaa ggtttgaggc
     3001 ccgccgtgga acgtaaaggc acatttatct taggtacgtt gttcgttgca tcacttctta
     3061 cagcttgcag caagccggcg cagtccgcgg gagccggagc gcaagccggc ggcgcgcagg
     3121 ccatgccggt gcagacggaa gtggtggcgc cgcaaccggt accacgcgcc gatgagtatg
     3181 tttcgaccat caagtcgcgc cgttcggcga ccatcaaccc acgtgtatcc gggaacctga
     3241 tcagtatcag cgttcgttcc ggagaccgcg tgaaagctgg acaagtcatg atggtgattg
     3301 atccggtaca gcaggaggcg acggtccagt cgctgaaggc gaccgagcag cagaagctcg
     3361 cagtctacca atacaaccag gtggaggtag agcgccagcg caagctctat caggagggcg
     3421 taaccagccg cgacgcgctg gaccaggccg agcagtcgta taagaactcc aaggccgatt
     3481 atgaagcggc ggtaaattcc cgccaggcgg gcgagaaaga gctgggtttt tacacgattc
     3541 gcgctcccta tgacggaata gtgggcgata ttcctgtcca cgtgggcgat tatgtctctt
     3601 cttctaccct actgaccacg gtggacgaga acaaggacct tgaagcctat atttatattc
     3661 caacagagcg tgcccacgag gtacacatgg ggctgccggt tgtgattgaa gacaacagcg
     3721 gcagggaagt tgagcgtaca gccatagatt ttgtttcgcc gcaagtggat gaccagctac
     3781 agggcatcct ggcaaaggcc ccggtgcatt ctccgcaatt gcgtaccgag caattagtca
     3841 aggcgcgagt gatctggtct acagcacctg ccccgacagt cccggtgctg gctgtaaccc
     3901 gcatcggcgg acagaccttt gtctacgttg ctcaggacca gggcgggaag tatttcgcaa
     3961 agcaacgcgc agtgaagctc ggcgacacaa ttggcaacag ctatgcggtc gaaggtggat
     4021 tggagaacgg agacaaggtg attgtctcgg gaatacagtt cctggtggac ggcgctccgg
     4081 tacatccttt gggatgagca gattctgctc ataatgcctg aggacaattc gggcacgctg
     4141 caattacagt cacacatctg gatggagcgg cgttctacgc cttcgttcaa gatgacaaac
     4201 atttatctaa gtcgagaggc agcatagcca ctccgtgaca agtttttaga gggtttgcga
     4261 ggggtttcgc gtggtagatt tcttcataca ccggccagta tttgcaacgg tctgcgcgct
     4321 gctgattatt cttgcgggcg cggccgtgat tccgacgttg ccaatttcac tctatccgca
     4381 gcttgcgcca ccgcaggtga cggtgacgag caattatgtc ggcgcaaacg cgcaagtagt
     4441 ggaatcggcg gtcacgattc cgctggagca acagatcaac ggcgttgagg gaatgcacta
     4501 catgacctcg accagctcaa atgacggcac cagcagcatc aacgtcacct tcaacaccgg
     4561 atatgacctg aacattgccg ctgtagacgt gcagaaccgc gtgaacacgg cgcaggggcg
     4621 tctgccacag gagatcaaga acaccggtat caccattaca aaggcgaacc cgaacttcgt
     4681 ctttgcagcc ggattttatt cgcctgacgg aagcctctcg aaccagtaca tctcgaatta
     4741 tctggacgtt tatgtcaaag acccgctgaa gcgcatcaag ggcgtcggcg atgtcgtgat
     4801 ctttggtgag cgcaaatatg caatgcggat ctggctggat ccggcgaagc tggcagcacg
     4861 caatctcact gcgacagatg tcatcaatgc tatccaggaa caaaatattg agattccttc
     4921 agggcagttg gggcgtccgc ctgctccttc aacgcaggat tttcagatta cgctgcgcgt
     4981 cgtgggccgc ctttccgacc cgaaggaatt tgaacagatc attctgaaga gcactccgaa
     5041 tgggcttgtc cagctaaagg acgtaggtca cgcagaactg ggcgctgaga cctacgatag
     5101 caatctggag tacagcggac atgaagccat cggtattggc gtgcagcagc tttccgatgc
     5161 caatgcgctg gatgtagaca aggcggcaaa ggcggcgctg gtcgaactgt cgaaatcctt
     5221 cccgcctggc atgaagtacg tcatcgcctt tgataccacc acggtcatcg gcgactctat
     5281 caaggaagtc atctctactc tggaagaggc gattgtcatc gtcattgcgg tcatcttcct
     5341 cttcctgctg gattggcggg cgacagtcat cccggccatt acaattccgg tgtcactggt
     5401 gggaacgttc gcgtttatca aggcgtttgg cttctccatc aactcgctga ccatgttcgg
     5461 catcattttg gcgacaggtc tggtggtgga tgatgccatc gtcgtgattg aaaacgtgca
     5521 gcgccacatt tcgctggagc attgtgatcc gcacaaggca acatctgacg ccatgggcga
     5581 ggtgacgagc gccgtgattg cgacatcgct ggtgctgatt tcagtgttca ttccggtgtc
     5641 gttcttcccg ggcacgacgg gcatcctata caagcagttc gcgatgacga ttgccttctc
     5701 catcgcgatt tcagccttca acgcgttgac gctctctccg gcgctggctg cgcttttcct
     5761 gcgcggtgag gaagagaaat tcaggatgct ggactggacg cgcatccgtt tcctctccga
     5821 aggatatgcg gcatttgtgc gcgccattga cggcgctatc cattggctgg gcaggaccta
     5881 tgccagaatc attcatgtaa cgctgaagct tcgctatgtg gtcctgctgc tcttctttgt
     5941 aggacttgca gcaacgatct atatgtatcg gactgtgccg accgggttta ttccgcagga
     6001 agaccagaac tatctgatct gcgtagtaca aacgcctccc ggagcgtcgc tgaactacac
     6061 gactgaactt tcacacaggg ccgagcagtt gattctgcag gacccggatg tgttcgggac
     6121 ctttgccgtg cctgggttca gcttgtccgg cggcagcgcg ccgaattatg ggctgatctt
     6181 tgttcctttg aagccgatgg acgagcggcg cggaaagggg cacagtgcat ccgacattgc
     6241 gcagcgtttg tctccgaaat tatttggcgt gcctggcgga attatcgcgg tcttcgagcc
     6301 gccagcggtg cagggtatcg gcagctttgg cgggttcgag ttccagttgc aggacatggg
     6361 ccgcaatacg ctgcaggacc tagacatggt ggcgcacaaa atcgtggcag ccagcagaca
     6421 gcgtaaggac ctgactggat tattcaccag ctataccgcg aatgatccgc aactgctgat
     6481 tcagattgac cgagaaaaag caaaagcaat gcaagtgcct atcagcgagg tcacgaacgc
     6541 gttgaatgtg tacatggggt cggagtacat caatgacttc gatttcaaca accggtcgta
     6601 tcgcgtgtat gtccaggccg accagccctt ccgcatgaag cagagcgacc tgcgtcagtt
     6661 ctacgtgcgc gcgaataacg gaagcatggt gccgctggac aatatcgtga acatctcgca
     6721 gacgtctggg ccgcaggtga tctatcactt caatatgttc cgctcggcgg agattgatgg
     6781 ttcggccgcg ccgggataca gctcagaaca gggtttgaag gcaatggaag agctgttcga
     6841 gcagaacaaa ctgcaaggca tgacgtattc ctggagcggc cttgcacttg aagaagtgga
     6901 atcgcagggc aaggcgatca tcatcttcgg tctgggaatc atcgtcgtgt atctgacgct
     6961 tgcggcccag tatgagagct ttgcgctgcc tttcatcatc atgctgtcgg tgcccatggc
     7021 cgtgctgggt gcgctcggat tgatttccgt gcgtgggttc ccgaatgacg tctatgtaca
     7081 gattggtctg ctgatgctga ttggactggc ggccaaaaac gcaattctga ttgtggaatt
     7141 tgccgagcag ttgcgcgagc atgggcgctc cattatggat gccgcaattg aggcggcaga
     7201 gttgcggtta cggcccattc tgatgacttc gatcgcattc attctgggcg tgttgccgct
     7261 ctatttcgca accggggccg gggccaaagg gcggaactcg gtgggaacgg tgattgtagg
     7321 cggcatggtg gtgtccacct tcctgaacct catcttcatt ccggtgctgt atgtgctgtt
     7381 gagcacgctt ttgagcaaga tgaagcggca gcgccgcaga ccggaaggtt gcgatgaaca
     7441 ggaagtaggc gaggaaagcg ccgcattatc gtcgtaagtc ctggtgcttc accccggctc
     7501 aggaggggcc aaatgtcggc ccctttttct ttcatcacct ttcaaagggc agattgcgat
     7561 atacttaagc gttgggaaac cttgtgcctg tgcttccgcg cgggcccgtg tcgaagagac
     7621 gtctccccaa gataagaaac aaggactgaa aggaagattt atctgtgctg atgattcgtt
     7681 tggcgcgttt tggcgctcgc aaacagcctt attaccgcgt agtagtgatt gagaaggacc
     7741 gcgcccggaa tggccgctca gtggaagtgg ttggaacgta caacccccgt accaatcccg
     7801 ccacagttaa tctgaagcgc gagcgcattg actattggac cagcaagggc gcgcaactct
     7861 cagataccgt tcaaaggctc ttaaagactg ccgttgcctg aaggtcaagc gtttgttctg
     7921 ctccaggcag tggatggtaa ctgtctgaag taataccaaa accggcacca gaaagcactg
     7981 tcctcccccg caatgaactg ttactatggg ccagcttgac acacaaaaat gtgtttatgc
     8041 gggggcaagg aatgacgcaa gtacagaaac cagacgatat gcagaaattg gtcaccgaga
     8101 tagcgaaggc tctggtggac gaaccgggtg cggttgttgt tgaagcggta gagaaggaag
     8161 aaagcacggt gctcaggctc cgtgttgcac cttcggatgt tggtaaggtc ataggaaagc
     8221 agggacgcac ggcccgttcg atgcgtacga tcctgggcgc agtcagcatg aaacaccatc
     8281 accgttatac gctcgatatt ctggaggagc acaagcaggt agcgactcca ggcgagtgat
     8341 tctctgattt tcccctacag agttcatgaa caatgttccg caatggattg taattgcgca
     8401 ccttgtccgc ccccaggggc ggcggggtga ggtccttgcg gacattctga ccgattttcc
     8461 cgagcgattc agcgaccgca gggaattgat gctgcttgat gccgggggca gacctttgcg
     8521 ggctgtttcc ctagaagctc attggctgca caagggcagg gttgtgctca aattcgctgg
     8581 atgcgattcc atttctgatg ctgaagcgct gcgggggctt gatgtggcaa tttcgcgtga
     8641 gcagcgtgtt cctcttgatg cggattccgt ttatatctca gacctgattg ggtgttgtgt
     8701 tgttctgcct gatggcgccg aagcgggcaa aattgtcgct gtagaccgtg aaacaacgga
     8761 cgcgccgctg cttgttctgg aaacgggtcg cagcgatcag gtgcttgttc cttttgcgaa
     8821 gtcctatctg cggcagataa accttgagac gaggcagatt gtaatggatc tgccagaagg
     8881 acttctggat gtgaattcga gttcttctgc tgaagaaaac taaaaacccc ttccttaatc
     8941 cgcggcgttg cattcacgaa tgcctgcatg ggcagtgggc gattcattcg ccccatcggt
     9001 ggaaacaacg cggaggcccg gcttgcgatg ggctggcttt aatgcccggg agcaggggcc
     9061 tgagtttggg tctgctccgg ggtggatgtg ctttgtggag gacggtacct cgtcaatctg
     9121 taatagatgg gtgtgacctg gaaaggcatc ttgtcgctgg cgctgacaat gactgcagcc
     9181 ggcgcatgca actgatagac gcggtcgtcc catgcatcaa tcctgagatg gcttcccaga
     9241 ggcagggccg caatctgttg cggattgttt acctgaagcg caggagctcc ctgctgcagg
     9301 gcagcaagcc agggctggcc atcgctgcgt aattccgaat ctccgaggcg tggcatcagc
     9361 aatgagggta ttctttgctg catattgacc agttgggcca ggaacagtcc tacattcccg
     9421 gacttatctc catacaccga gcctttgaac agttcgactg ccttctttgc agcttcctca
     9481 ttctgttcta atgagtggtt catggctatg cgctggaagg tggattcgtc gggaaacagc
     9541 aagcggtcgt taaaggcata gcgggtatca attccatgcc ccaggacaat gtgggccaac
     9601 tggaaactca gaactgccgc caggtcttct tcgtaaggaa gtacatctat taggcctttg
     9661 ctcaaaacaa tcgtgttacc aatggcaacc gattcaaggg tagaggtaag catcacgcgg
     9721 caatgtactg gttctggaag attaagctta ttgccaatga tgatgttgtt tgtgacggtt
     9781 tccagcacat gatcgaagtc gctgggaggc gcaagcaatc cggcgcgggt cagacgttcc
     9841 agaacatttt gttcgctctg gttttgccat tggcgcgttg cctgcaatgg atttacatcc
     9901 tgcgtgtttt cgctggcatc ggtcgcgttc tcaacaatga cgctttcgtt ttcgctttca
     9961 cgcgagggaa gttttaaccc atagccccag atataggttt gaccacggaa gtcgaggtcc
    10021 tgcacgtaga ttgcaacagg cagccagatg tccggctgca cattcgccct ccagctatcg
    10081 aaatggaagt agaggttccg cttgtttcca ccgctgaatg tgcctgtaaa gcggacaata
    10141 ttcccgctct catcatcaat ccagacgcgc ccgatgaacc gtcccattcc agcatgtggt
    10201 ttaggctgga catcaaatac agaggtttta acttctccaa ggaattcgtt gcggacaaaa
    10261 gcaaagctat agtggttttg atcaaagctg ctggcatcca caaacatcat gtccataaag
    10321 ccatttgccg agaaacgcag gcggaacatg ccggtcagca ttccaagaaa gcctttggag
    10381 ccggagaaca tcctcttctt ctggcccttc tgagcaaaaa ggttttcatt aaacacaccg
    10441 ctgaaatcta cccggcccag caggtattca tccgagtatg gaacgctgcc taactgcgga
    10501 tctggctgca tatgttggat ataggtctgg accagtggcg gatgctcaga aatcgtcttg
    10561 acgagaagtc tttcctgggc cgcagctttt tgcaaaagcg ccatctgctc gggggtaagc
    10621 ggaggtgtct tctgctggac tactttgtca gcgctgcaaa cgaatgaaaa acaagccagt
    10681 attgccaggc acgtcaattt aaattgctta ttagtacttc tcattaccag accctttcta
    10741 aacttctaac ttactatgag cgagtcaacc caataccagg ccttggcttg catccggact
    10801 ttcacggaag aggacaaccc tgtagtctca tcaagaacga tttccatacc tactggtgtt
    10861 caaaccctgt tgccacgcct atactatgga ccacaatccc ggcgcaagga ggcagccacc
    10921 atactttcga tctgcgcttc ttgagccagt aacttactcc tgaactgtat gcgaccaagg
    10981 cgaaattcac actgagttgt ccaacatgtg aagacggcat aagaggattt gcttcgcggc
    11041 agcgatgatt gcgaatgcag agctgggtca tttcaacgtc aaatactgcc atgcccaact
    11101 ggaatccatt taataggaag tatttcttat tcgcaactcg cggaacctgg aggggagatg
    11161 gagtgggcgc cacggtggca gaggaaggag ctgatggcaa aagtgctgcg gggactttgt
    11221 tgtcgctgct tgccgacgcc agttctgcat gactggccgc cacatggggg gactgcggtt
    11281 cctgcgccga acacttcaat gctgccccag agatcaatat aagccccaat actacgttat
    11341 gcgccgcttt catctcttct ccattcgccg ctggagcggc tgatggagtt atcggcgcga
    11401 agaggaagcg aaacaggcag atactacgcc tgtttgctta agattttcta atgatgtcct
    11461 cccggatgaa gtgttgtcct gtgcccgtaa gaatgggcca gcttttcatg caaggtgtga
    11521 tacacaaacc aggaatgcac acgggcataa cggcctaaca aatttcttgg atcttgccat
    11581 agccgataaa tccactccat cccatggtgg gtccaggaag gagcatcgcg tttcagtccg
    11641 gcaagcacat caaagctgcc tccaacgccc atggcaacac gcggagctag ctcttctctg
    11701 tggttttcga taaacaactc ctggcgcgga gaccccatag caacaaataa gacggacgct
    11761 ccactctggt taatactcga gatgatcgag tcttcttcat gagattggaa gaagccattt
    11821 ctggcccctg cgatttgcaa atgcgggtag caggcggaga cttgctggag catcctgttc
    11881 agtacttcct ggcgcgcgcc gaagaagtag accggttccc gccattcttg cagccaggga
    11941 agcagggaac gaaagagtcc tatgccgcgg acattggctt tcagaggggt ccctaagatt
    12001 ctggatgccc ataccggacc gtattctgtg atgaccatct cggcattccg gatgatcctg
    12061 ccaagaccag gatgatggtc cgcgagccag aatttgtttg tattaacgac cacaatatga
    12121 tgcagatatg gctcggacaa aaagacggcc acgcgggaaa tcgcctcatc cagagtcacg
    12181 ttcgtgatgg gtatccctag cacattgact acttctgtgc gggatatttc actgggttca
    12241 tgcagtatct caacagtgga catcgtggtc tcctgaactg ctgtttttac atcagcttca
    12301 ctcaaaaaaa tctgtcagct ctgactccaa tgcgtctcag ttactaaaga gcaggggaat
    12361 gggcccctgc ccgaagcctg aaccagggtt ccttttactg ttgctccccc ataagtacgc
    12421 gaaatctgta tgggtgtctc ggcccacttc aggggaagga gcgaagtgat ctgcatagat
    12481 gcatttcccg gtccgcgcag aaggaacctt ccgttatctt gtgccgtaac ctgccatgga
    12541 gaagggataa tccagcgaag atcaacgcta tgttctccct cgccgagcag atcatcctgt
    12601 acgcgcaggg cgccattgga cagccgtaca gtccggcggt gttccactcc cagcctttca
    12661 tagccgctat gcgaggcgca catcgcgatt gcatccgcgt tgtctgttac ttcaatggcg
    12721 ctaacggaag cctcattgcc aatacagaac acgtaatgcg gatcgaggtt aatagtattt
    12781 tgctcctcgc catctataac tactgtgttg tgggatgccg tggaacgata ttgatttcgt
    12841 cttgccgtat ctcgcgtgta gcagaaggtc cccgaatcgc tgaacagctc ttttccatag
    12901 aggcggaaaa tcacactgag tttgtcgttg tgcgtgtggc ttccaccgcc atgaatacca
    12961 ttcggaatga cgcaaaaaac aacttcagcg ttgtctctgc gcaccacgcc aattccagat
    13021 tgcggaaaga tggtcttttg tgaaaacgaa acgcgctttt ttaccggaga caggccatac
    13081 catgccgcgt cagaagggtc gccatcatat tccaaaccaa agagaccatc acccatgcca
    13141 atcattccgg gaacaaagag agagttccgc tcaccggggg caaggctcgt catctgcttc
    13201 aggtcactgg tcagcagttc gacgcgtcca tcgtcggaat ctccaacatg tggcagagtt
    13261 gcagatggcc cggcgatttc cgcaagatac cggtacattt gttgcaagcg ttctgcaaat
    13321 tgctgctctg gtttaaggtt tgctgcccgc atcagaagat atgcgctggt gaacatctgg
    13381 agcacgagga cgtgatagcc caacgaagcc tcgtaatcgc caccatcttc ataaacctgt
    13441 cgcaagattt cattttgtat tcgtgacaga tacaaatgcc tacgccgttc catgcctggg
    13501 ccatcgagaa acatggccat gcacagaagc ccggcgatgt tgcctaaata atggttgctg
    13561 cggacgaggt gggaaaactc aagacaggat tcggtataaa gcaggtgctc ccaaatggcc
    13621 tgtgtaacct tccgccccca gtcttcttct tcaggccgaa ggggctgcag gagcgcgagc
    13681 gtaaagcaga gactcattcc gcgcagggcg ctctccatgg caagtgtcca attcacgccc
    13741 atgcctacag gatttttagc gagccaatct gagagcaggt cttttgcaat ctcacgatat
    13801 tgtttttcgc cagagagcag ataagctttg gccagcactg gaagaaattg caaccgtgag
    13861 agttcccatg gtgcttttac atcggagcca ttgtgccgca ccacgggctg gagctgggct
    13921 gctggcagtt gttcccactc aaacccgctg acgaaatcgc ggttccactg gggagtaagg
    13981 ccgaaaaaga ttgtctgata accaggaaaa ggaaaccggc cgctgcaaat cagattggca
    14041 aaatccgtaa tggctttagc ggattcagga gcaatctgtt ccagtctggg ctggtaatat
    14101 ggcaggtctc gaaaccggac ctttgcatcg ccgcggcgga ttgctgcccg caaccgggag
    14161 gagcggtaag gcttccatac acgccatgcg gcttcgcgca ctaaagcgcc cgagggctgt
    14221 ctgcgtacta gggggagaat ggcgcgcaga ggtctcatga tccgatcgct accaaatcag
    14281 atgtccttct ttgaatattt ctggtccaaa taccgcaaaa tattgcgtga acctgaatcg
    14341 tagatatcga acgggcatga tcctggccag gaactgctgc atcaattttc ctttccatct
    14401 aactaacgat gacttactga aattgcactg gaattttgag tgcaggtttg ccctgcaaat
    14461 tttagcgaaa aaaatgtaaa tgcttccatg cagacttggc aagcaggaaa ggcaaatttc
    14521 aatactggtc tctggacagt aacttactct gccagagata ggattttgac ttttatgaaa
    14581 gagttatacg gtcagacaaa agaattatgc agtcaaacaa ggcctggagg catcaatgaa
    14641 ggtttgctgc catagctcca atgtaagcag agaccatagc tgcaaggtgt aatcgcgctg
    14701 ccctgtgcga ttgtcttcta tcatcttcgc cacgcgccca gggcgaaaaa ggccgcgttc
    14761 ccggacccgt ttctctgaaa gaacatcgcc tagcatttcg ctgagctcac tgcggagcca
    14821 actacgcact ggcagactga atccggcttt ccggcgatag atcacgtctc tgggtagcac
    14881 gccttccatg gcttttttca gaatgtattt ctggatatga ccgtggagct tgaggtgcga
    14941 aggaacccgt cgcataaaat caacaacggc attatcaaga aacgggacgc gcgcttcaat
    15001 tgaggctgcc atgcttaact tgtcagaata ggccaggttc aggtcaggca aaaatgtctg
    15061 ctggtccaca tagagcatct gttccaggaa atcgagggat gcagcctcgt cgaaatgttc
    15121 ttgtagtatt tcttcataag gttttgatat ggcgaaaata tctggattta ataactcctg
    15181 caactcatct gttcgtaagt aggaacgcat tccaataaac tggccagctg gcggtaaatc
    15241 aataagcttt aatacccggg taagataccg gcaatatgcc atgaccaggc caggttgcag
    15301 gcctggcact cgagatgtat tttttgagat ggtttggagt gccgcaagaa gaaacttttc
    15361 ccgtacaggg cgcgggaggg tgcgtatcca tgaaggaaat ttttctacta agtggacgcg
    15421 ataaccagca aataattcat ctcctccctg cccactaagc aggaccgtta cgttggccct
    15481 ggcctcttta caaatcagcc atgtagcgat ggcggcatgg tcggcaatag catcgtccag
    15541 gtgccagatt gctttcggga gcaagctggt tacgtccggg tccactacga tttcatggca
    15601 ctcggcgccg aagtgctgtg caaccttcct tgcgtaaaac gggtcttcgc cacattgttc
    15661 aagtctggcg tgttcttcgc ggaaagcgat ggtgtaacag cgcaggggcc cgctgtggac
    15721 ctcggctgcg caggcaagta aagtgctgga atccagtcca ccagaaagaa agaagccaac
    15781 gggcacatca cttactaact gccggcgaac agcatctttc aggacctccc ggcattgctg
    15841 cgtcagatcg cgctcgttgc cgggcagaag gcctgctggt tttgagttgt cccagtaacg
    15901 ctcaatctgt atctttcctt ttttccagat aagagaatga ccgggctgaa gtttccggat
    15961 gttccggaac attgtatccg gatgaggaac atacaaaaga gacaaaaagg aacgaagaga
    16021 agcgatatca agctcgggac agatgtcggg cgccaataga atcgctttga tttcggaggc
    16081 aaagtacagt ttgctgccct gcagggtgta atacagcggc ttgacgccca tacggtcgcg
    16141 ggcgataaaa agcgtgtggt ctcgtgtgtc ccagatggca aatgcaaaca tgccgttcag
    16201 atgctgcacc atttccgggc catatttcag ccagaggtat ggcagtactt cagtatccgc
    16261 gtgggagcga aagacacatc catctgcgag tagctcttcg cgcaattcgt gaaaattgaa
    16321 taactcgccg ttataaacaa cccatatgtt tccatcaggg cttgccatcg gctggtgtcc
    16381 agctgaactg aggtcgagga ttttcaggcg gttatttccg attgcaacag attgatctgg
    16441 agcgttgccg ctgctcaggt atgttcccca gtcgtccggg ccgcggtgcc gggtaatctc
    16501 gagcattttg tcgagcaggt cagcattcct tgtaccggcc atgccagcaa tgccgcacac
    16561 ttatacctcc gttcacctgt tgctcatcca aaataaaacc aggatgcctg ttcaggaaag
    16621 cgccatagct tcggggatgg tggttttgtg gttctgccaa tattctttca agacccgcac
    16681 aatgcggggc gcagttttgc cgtcccaaag aggcggatgc gctggcctgt gcccattcgt
    16741 tccgagctta tcgagcactt ccatagcggc ccgcaaaagg cggtgaggat cagctccgat
    16801 caacttgttc gtccccagaa gaacagttac cgggcgttca gtgttgttgc gatacgtaag
    16861 acaaggtatc cccagatagg ttgtttcttc ctgtatcccg ccagaatcag taatgaccat
    16921 agcagcctgt tctaccagtc ccagaaaatc cagatacgag gccgggggta tcatccataa
    16981 gccatgctcc gggatgcgtc ctccgttcca ggtttttacg caatccagcc cagcttcaga
    17041 aatcttcacc gcggtcctgg gatgcacagg gaaaatgacc gggacccgtt ttgcaacctg
    17101 agcaattgca tccagggtac tggacaactg ctgcttgtta tctacattgg atggccgatg
    17161 aagcgtcaag accgcatact gccggggaac gagaccaagc tcaccacgca gcgcggactt
    17221 atgtgctagc tccagcgcgc cgaccagaga gtcaatcatc agattgccga caaagtgtac
    17281 tttttccgct ggaattcctt catgagcgag attctgattg ccgctttcct cggttgtaaa
    17341 gagaagatcg gaaatcgagt ctgtaagtat ccggttgatt tcttccggca tagtgcggtc
    17401 aaagctgcgc agacccgctt ccacatggac cacaggtatc ttttcctttg cagcgaccag
    17461 agcgcatccg atggttgaat ttacatcccc gacaaccagg acaatattcg gacggtcctg
    17521 ctgcaccaga tcgccaaagc gtcggataat ttctgctgtt tgtacatggt gcgtcccgga
    17581 gcccacttcg aggttgtaat cggggtcagg catccggagc tggtcgaaaa acacctggga
    17641 catcgagtag tcatagtgct gcccggtatg cacaaggaca ggcccgattt caggataacg
    17701 tcgcatttca gcaattaagg gtgcgatctt catcatattc gggcgcgcac caacgatgtt
    17761 cataatccgc aaagaggcgt ctggcatttg gggagggccc tccggtttgg gatcactgca
    17821 gattcataaa atttatccgg cgtatttgta acaggctgta tgttgaagat gcaagttatt
    17881 atgtttgcgt ttaccaggaa ttttgcctgg taaccccctg attgttgttt atcaggccta
    17941 taatcgttcg atagttagga ttttaggcgc gcccgcgagc gtagacggga ctgtttcaaa
    18001 tttccccata aagggacctg ctcatgtgcg gaatttccgg agtcctggca tttcagaaca
    18061 gccgctttaa cgtgaccgaa gaatttgttc tccgcatgag agacacgatg acgcatcgtg
    18121 ggcccgacgg aggcggacta tggatatctc ctgaccgcaa ggttggtttg ggtcacagaa
    18181 ggctctccat cattgatttg tctaatgccg cctcgcagcc aatgagtaac gctgacggct
    18241 cgctccaggt tgtatttaat ggcgagattt acaaccatgc tgaaattcgc caggagttgg
    18301 tgcaactcgg acatcgccac tggaaaactg atcattcaga caccgaggtc attctgcatg
    18361 cttttgagca gtggggaatt cagtgcctgg aacgcttccg tggaatgttc gccatcgggc
    18421 tttgggactc aaacgcaaaa cgcctttggc tggtccgcga ccggataggg atcaagccgc
    18481 tttactacag ccttcatcat gggcgtatta cattcgcatc agaaattaag gcgctgcttc
    18541 aggaccccga tcaggtccgt gcagtcaatg aagaagcctt gttccactat ctctcgtttg
    18601 tgactacgcc accgcccggc actctgtttg aggggatatg taagttgccg cccggttcat
    18661 ttctgaccgt ggatgcttct ggacaatacc gcgtagagcg ctactgggat gtgcttgacc
    18721 acacctcacc tttaacaaac gtgccagaag aagaaatcgc cgagcgattg atcacggaac
    18781 tgcgcaccag cgtggccctg cgaacgctca gcgatgttcc ggtaggcgtc tttctttccg
    18841 gcggcattga ttccagcacc aatgccgctc tcttctcgga atgcgcttct ggccgtgtaa
    18901 agacattttc tgtcggttac gacaaaaatt atcgtggctg cgaaagcgaa ctgcctcttg
    18961 caaagaaatt tgcagacagc atcggggcag aacactatgg gagggtcctg acacaagatg
    19021 actttctgga atttctgccg cgcatggtcc atttgcagga cgagccgatt gccgatccgg
    19081 tctgcatgcc ggtgtattac gtgtcgaagc ttgcccgaga caacggcgtg attgttgcgc
    19141 aggtgggtga ggggtcggat gagcttttct ggggatacgg atattggcgg cagatgtttg
    19201 atctagtccg ctggaattct tatccagtgc cgcgcgctgc aaaaaagctt ggtctggccg
    19261 gactaaggat gctgggcaaa gcacaaagtc ctcagtacga ctttttgtgg cgtgccacac
    19321 aggggctgcc ggtcttctgg agcggctttg aaggatttac ccacaatgca aagatcgaag
    19381 tgctttcacc gagattgcgc gagcggttcc gcgggaggac ttcctgggag gcaatccgcc
    19441 caacttacga gcgctttcaa cagaagtgct gggaaattgc tccggtaaag tggatgacat
    19501 atgcagattt gaatcttcgc ctaccggagt tgttgctgat gcgcgtggac aagatgggca
    19561 tgggcgtaag cctggaggca cgcgtcccgt ttcttgacca caaatttgtg gaactggcga
    19621 tgagtattcc ggaatccatc attacgaaag gccggcaatc gaagcacatc ctgaagaaga
    19681 ctgtgcgcgg aattgtgcct gacgaaatca ttgaccggaa aaaacaagga ttcggcgttc
    19741 cgatttacga gtggttcttt gaacaatttg gagcatttgc aaggcgagaa cttacggaat
    19801 tctgcgagaa aacggacttt ctcgacccgg cctccgtcat gaaactggtg gaagaaggcc
    19861 gtggatggca tgcctggttt ttgttgaatt ttgcgctgtg gtggaacgag tttattgatg
    19921 cacgcgggcc gggctttgcc agcgcggaag aaccgctggc cgttgcccgg taaatggccc
    19981 ggattacgcg cttattttcc tggacacgag cgaaatcagg tcgcctacat ttttcagtcc
    20041 agagacttct cccgttgtga agcggatctt aaactccttt tcaatcgcca cgatcaggtt
    20101 gatatgggtc agggaatccc agttctcaac atcatcggcc gtcagttcat tccgcaggac
    20161 gatggaatca tcatcaaaca cttcacgaaa aatatcagtc aatttttcaa ttaagttgtt
    20221 cataatcatg cgacggcctc agcctcaggt tcacaagact tgatataggt atttctggga
    20281 acataggagt ccaccgctag tatccagcgt gttgcctccg gcgtatcagc agtttgcaaa
    20341 aatccaaacg aacggaaaaa atcgcgtacc atcccgtttt tggccgtcgg gatatatgtg
    20401 ccggctacat gctttaagcc atttttagca gcgatggcaa aaacttcatt cataacatac
    20461 tgctccacgc cacgagacag aacacggcaa ctcatcagcc agtcggtaat ttccagacac
    20521 tcattgtccg gtttcagaat aacgactgaa atcagtccgt ggtcccccag tttgtcagac
    20581 aatttgacgt atagcgggaa catatccgga tcatgcatca ggcgttcgca ttctgcttcg
    20641 ctccggcggc gcgtagtcag attgaattga ttgcttctct ggatgagctg cgcaattctg
    20701 ggcagatgga aagaatcaaa acgggagacc acaatacgca tatcaagaga ctggagaaat
    20761 tcttcgacag aagcatagct ggcagcaaca gcgcggcggc tggcctcctg tttatacatc
    20821 tcagcgcgtt gcagatcttc gctggaaaaa gacgtggttt caaaaagatt cagctcacaa
    20881 agaaatctca catattctga tggatcgtcg ggtaattccg gaacaatgat gcctggaaca
    20941 agctcccgta ccatgtttcg ttcaaaggga ttgtcgtcaa caaacaccat tgaatcaaat
    21001 ccgatattca gggcctgctg gattgccctg atgttttcgg ccttatcgtt ccagttggca
    21061 acaaaaatag taatgtcttc ttttttcagc accatttccg ggtgggactg aaagggcaac
    21121 aatgcgttct gctcatcatt tttactgcat actgccagaa gaatgccgcg ctgcttcagc
    21181 tctttcaaaa agcattgaaa acggtgccag gcttcacctt cgccatgggc gctcaatttg
    21241 atgccttcaa ggccatcatc gccaatcacg ccgccccaaa gggtattgtc cagatcgagg
    21301 accatgcact tgaccactcg tccgcgcatc gccatcaaaa tatccaccag attctgtgca
    21361 acatagggaa gagcatcgaa agaacagaat gtcttccaca tgtcccagaa gcggtcgtcg
    21421 aaaaagtgct ttcttccgat ataagaagct actccctcga tatcaagcag caagacacca
    21481 ttttgtccac gcgccttttg tgtaagtcct gtattcagtg catgaacagc cgaataaaac
    21541 gagtctgcgg ctttaaggtc gtaattcccg aaatactttt ccaatggaag ggcgaagttg
    21601 ctttgtatga tagtggcgtt gcagtgggac tggattgcat tccagatact ctgcgttttt
    21661 tcaagaaagc catccgtgaa gctcgctgcg ctcccgttcc gttttgcgaa cgatgcgcgc
    21721 aatgcctgtg ttgcattcag aatgaccaca acatctggtc caaacgcata gaatctggaa
    21781 gatgggttat aggcttccag ttcgacagca tcgaatgcgc cctcgtagat ttccgccgaa
    21841 aaaccgttcc tgtccaaaat ggccttaaga agagggacaa actgttgcgt tgccgcatcg
    21901 ctaagtaacg ccaatttgag ggcaggtttg ggtgatgctg ccgataattt cctgcttgcc
    21961 gctagaagcc gacgataatc gaggtcgggg aggttgtctg tttgcatgtt tctctgatgt
    22021 acaaccagca atcatgcatt tccgggacaa tgaaaaactt caagtctcta ataaaatggc
    22081 gagcgcttct ctgtgttgga tcattgtgtt cgctgccaaa aataacactt agagtctacg
    22141 cggaccagaa gatcttttat gccatgttcc tgccgaaatt catccactgc tcttcggcag
    22201 tatgtccatg tatcttcacc ataatcatca ataatcacat atccgccaat agaaagttta
    22261 ggataaagat aagtcaggct gtcccgggtc gagtcgtagt aatcgccgtc cagccgtacg
    22321 attgccagct ttgaaatcgg cgctgaaggt aatgtgtcgc agaaccatcc cttcagaaaa
    22381 cggacccgat catccaacag accaaacgcc tgaaaatttc tctgcacttc ttccatgctg
    22441 acagcaaagt gattgtagac cgtcttcatc acttttccgt tatggacctg ggcctccagt
    22501 ggacacttgt cggcatctgg agcgggcagt ccttcaaagg aatcagccac ccataccgtc
    22561 ctgtcctcta ctccgtaagc tttgagcacg gcacgcatga aaatagtcat tcctccacgc
    22621 cagacccccg tttcaatcag gtctccaggg acgccgtgcc gcagcacatc ttcaatgcag
    22681 tattggacgt tttgaaacct gctcagtggg cacatggaaa ttgccgtgcc ttccatgtaa
    22741 tgacggataa aagcattcac ataccggagt tggttttcat cgtccgcatc cggttcaggt
    22801 tgaaacaatg tgttggtcag cgtcgctttc aagagatcga aatagagggc gaccggactg
    22861 acctgagaac tgaccgcagc actggcatcc atgacggggg aagtcatcct gtttgtcctt
    22921 ctacgctggc gtgcgtgaaa ttagatgcaa ataagcgcat cattgttttt cactcaaaat
    22981 gcgacaccac tgagaattac cagaccatat gtcccgtgca gccttccttg ctgcctcgtc
    23041 tgttaaatga aaaggatcat tataattttc ttcattcaac gtgaattcat tgtctacatc
    23101 gcagtcatag acgggggcca acttttccag actctgctta aagttctggt attggccgtc
    23161 cagcccaagt tcatttattt tgcgccttac atccgcatgt tgaggcggaa tgataaatac
    23221 aaatgaaata tggttttgct gacaatatgc aactactttc ttcaaatcct cagttaagga
    23281 acccagatag gcatcccggt ttattttcac tttcacgtat tgaagctggc tctgccagaa
    23341 cgctgctttg tttactgtag gcccaagatt ggtcttatgg tgaaagacag catctgaaat
    23401 gtcataaagg cctgcttcca acacggttga attcatgaaa tagagcaaag gattatgcag
    23461 tatggccact gctcctggta cgcggtcaga agagtaagcg ctgtactcca taaaatcaac
    23521 tccgaaatac acgcgacgta gcttcgtcct ttgcgccgca aaccagaaca tcgaaatggc
    23581 ctcgcgcaat gatccgccac ccgtagccag gtttgcgtaa ggctcgcctg ttatctggga
    23641 aattgtctct gcgggaaggc ggtctgtttg cgagtctcct accaggatat ttggcttggg
    23701 accgcggtta tatgtaagga catcccacag ggcctggtcc aacggtgcag catacccaaa
    23761 tcgcgtgtgt tctggcactg gagatggttt ggcaaaaaaa gagaaaggat ctacccagta
    23821 gataatcgga gcaaccacaa gaacaactgg aagaaagaag gcgatgagcc ggagccacac
    23881 acctcttagc tgacggccgt ctgtaccggc cagcagagtt tgggcgctgc tttgtgcctg
    23941 cgttaccgtt tcctgtgcat caacgggata catagagatt taaaactgca cataaataaa
    24001 gtgagtactt tgctggtgta acgtgattac agtagccagc gcggcatagt aaacggccca
    24061 gcgcatggcc aacggcaatg atgaaaacga cggcagaaat gcaacgacac ggctttgttg
    24121 ggcccgcaaa tagctgcaca attccaaaac aagagctgcc gtcaccgcaa tgcccgtgag
    24181 tccaaaatgc atacggccaa atccataata gacccgggtg acggatgcta cgggactttc
    24241 caggccatcc cagagatggc gtagctcata agagatattc acaaagcttc ctgctctgaa
    24301 gaatacaagt gtgaacgcaa tcatcagaaa cacgaaaaac ggaccaatca ggttcgtcaa
    24361 aacgtctgcc caccggtgtt tgcgataaaa acgtttgcgc gcagccgacg taaagctgtc
    24421 taccatgaga taaatgcttt gtatgacgcc aaacagcagg aacccaatat taattccatg
    24481 ccagaggccg ataagtacca tgtttgcagt gatgctgaga gcgagacccc aattgcccag
    24541 atttcgtgtg gccatgcgca aaggtgtaaa tacataatct gtcagccaga aggtcagaga
    24601 catgtgccag cgcctccaga actggctgat gctgggcgcg aaatacggat gagcaaaatt
    24661 ttcaggagac cggataccaa aaagccccgc tgtgcctacc gcgatgtctg tgagtgcagc
    24721 aaaatcaaaa tacaattcca gcgcatacaa aatggcagca aaccaaagca tgttggaata
    24781 gtggggctgc attccgtcta tgtaagagat gattgacgcg agttggtctg ccagtacggt
    24841 tttcttcaca attcccagaa gaatgcgccg gaggccagcg cgcatgagcg ccgaagtttc
    24901 tatgctgctc ctgggcagtt taaactcgtt tgcccgctgg atcggccctg ctggaagctg
    24961 agggaagaag ctggcgaagg cgacaaactc aaccggactg gaccacgcag gatatttgcg
    25021 ccaataaacg tctatcagat atcccatcag cttgaacgtg tagtaggaaa ctcctaaagc
    25081 cagaaaagta tgcgtaaacg aagagggaac cgtccctgcg gcgagcatgt ccgggagcag
    25141 tttgataaaa agcaaatacg ccagaagtac gacgagggcc acagtgaaga tgagccgacg
    25201 ctgaagactg gcggtatcca tctggtccat gtatctggcc actgcgaaaa ccgccagtgc
    25261 tgcaataaga agtacgcctg ctgaaatcgc actggaaacg gcataaaaca atatcgaaac
    25321 tatagcaaga agcgctggcc gtaagcgcgc tgggcaca
//
LOCUS       DASRBV010000149        18092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_159920, whole genome shotgun sequence.
ACCESSION   DASRBV010000149 DASRBV010000000
VERSION     DASRBV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 18092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18092
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159920"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2421
                     /locus_tag="VFA02_05680"
     CDS             <1..2421
                     /locus_tag="VFA02_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09375.1"
                     /translation="TPGFTLTAILTLALGIGVNAAMFSVMDQVLLRPLPYPNAQRLVL
                     MGAVRQQGGFAAVSLPDMKDWQARNHTFQAIGYFESNPVTLRFGDSSNVTTQVMTSTN
                     LFELLGVHPAMGRSFTAEDGKSGRSNIIIVTDRIWRNYLHSDPNILGKAVFINGDPYT
                     VIGVLPPAVGFPGDGLESIYAPVNPDDNGLSDRSSSALLAIGLLRPGVTVEQAGHEIN
                     SIHQQLLREYPKDESTALIRVVNYRDALTENSRSAIFALYGAIIAVWLIACANVAGLM
                     LTRTNNRRREIAIRGALGAGRDRLVQQFLTESLLISLAGGAAGAGFAAIALGLLKHYF
                     AHTLLYGGFIHINGAVCAFLLLASCISALLFGVVPAWMAANTPLQEGLREGTAAAGVS
                     RKQAFWRDASVVAEITLTLALLIAAGLMMRTLYQLRRAEKGFIADQVVTGMLFMPTHG
                     AWWTSPDPEKAANLVKMFYRPLQEKLLHTPGISGVGFTTVRPLEPGWDFVDNIIVNGR
                     PKPNKSEQPTAQLRGTDEGYFRVFGIRLLKGRYFDSQDTPDSPVVAVVNQSFVKEVFP
                     REDPIGKQIEIADAPGPRHWATIVGVAEDVRQRSMGEAPVPELDLNLMQFSPKDEMYP
                     IISSFMNIAVRTNLPAADSERIIANAIHDVMPDIALQNFKPMPQVVDDSMGNQTLAAR
                     LLGIFGIAALAIAIAGIYGLLSYSVSQRMREFGVRLALGSPRGAVIWLVLRHALLLLA
                     VGIAAGITIAIAASGVMRAFIYGFHGYDVLTVFAVALVLAACALLASYLPARKAARVD
                     PVVALRTE"
     gene            complement(2484..3692)
                     /gene="manD"
                     /locus_tag="VFA02_05685"
     CDS             complement(2484..3692)
                     /gene="manD"
                     /locus_tag="VFA02_05685"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HZU09376.1"
                     /translation="MKITKASVNICSPGRNFVTLKIETDEGIYGLGDGTLNGRELAVV
                     SYLTEHVVPCIIGRDPFQTEDIWQYLYRGAYWRRGPVTMAAIAAVDVALWDIKGKTLG
                     TPVYNLLGGKSREGVLVYAHAHGATVEEAIADVQKHMEMGYRAVRAQSGIPGLSSTYG
                     VPKGAQPYEPAERGLPSESRWSTELYLRHTPRLFEKLRMAVGEDVHLLHDVHHRLTPI
                     EAARLGKELEPFRLFWMEDPVPAELQEGFKIIREHTTTPIATGEVFNTIWDAHDLIRN
                     QWIDYIRMSVIHAGGLTHLKKVADFASLYHIRTGCHGATDLSPVTMAAALHFGIAVHN
                     FGIQEHMPHSTETDAVFPHGYRFDSGYMYPGDAPGLGVELDEKLATKYPYQRAYLPIA
                     RKLDGTLTDW"
     gene            complement(3689..4852)
                     /locus_tag="VFA02_05690"
     CDS             complement(3689..4852)
                     /locus_tag="VFA02_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZU09377.1"
                     /translation="MRHVDLTSAELASSETARRINRDIILQIIRTRQPISRADLARLS
                     GLQRSTVSQIIEQLLEEKWVREGALAHLPRGRRPTLVHLNDDLVVLAADIHPHQATVA
                     LVDLNGRLLSRSTLPIGKDPAKAVHGMTECMKRIRDAYPRKSVEGIGISLPGRVDPET
                     QRLVFAPNLHWPDFDIKGAFEREMRLHTEMENAATACLLSELWFGRMDGIRDAVLITV
                     SEGIGSGLMANGQIISGRNGMAGEFGHVTLDPSGPRCACKGTGCWEMFASCNAALRYY
                     SELEPKSKRMTFHELLSLAEEGNTQAAAALAKQARFLGKGLRTVVAGLSPEVVLVAGD
                     VTSAWGRFAPLIEAELKEVVLAGGPPRLLPTHEGDVARLRGAAALLLQRHSVQ"
     gene            complement(4928..5704)
                     /locus_tag="VFA02_05695"
     CDS             complement(4928..5704)
                     /locus_tag="VFA02_05695"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU09378.1"
                     /translation="MSQCIFNLAGKTAVIIGGTSGIGRSIALGLAAAGADVVASSRRL
                     EEVKQTAAEIEGLGCRSLAVASDVLDRASLQSLHDKVLEKFGRVDILVNSAGTTQRVP
                     TLECSEKDWNYILETNLTGTFRSCQIFGRTMQAQRYGRIINIASLATFVAFHEVAAYG
                     ASKAAVASLTRSLAVELAHDGICVNALAPGIFPTSLNADLLDSTPRGREMHMRIPMHR
                     FGHTDELVGAAVFLASDAASYVNGQIIVVDGGYLASGVNQ"
     gene            6031..9543
                     /locus_tag="VFA02_05700"
     CDS             6031..9543
                     /locus_tag="VFA02_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09379.1"
                     /translation="MLYLLLSSAVYAQVLYGSLTGTVTDSTGAVLEGTQVTVLDVQTG
                     TTQSATTDSSGIYRFTTLLPGTYKVTIAASGFTSQETAGVMVRANEIARVDAQLKVAT
                     ATQSITVTTAPPLLQTDKADVHTDLTSREIENLPIMGSQGANFQSLLRTIPGAGLTAE
                     TNSLAGNPQRAINTNMNGLSNQGNNTRIDGAQDAYPWLPANVAYVPPADAIESVNVVT
                     NSFDAEQGMAGGAAINVQIKSGTNKFHGSAHEYHTDQNFAARNYFQTDPKIFPHKNRN
                     NQNQFGGTIGGPVVKDKLFFFADYERTTQRQKAGPDTRTLPTAAMIAGDFRGLPGSPI
                     IYDPATGDQHGANKQQVSCNGVPNVICPNRIDPAAQAMVKLLQPLVSQEFQTSNGMNN
                     WTGSGTALFNRDNADFKVTYVPNQNSTVFGRYSFSKTLVFDPPLLGAAIGDATNGGQL
                     GNAPGLVQSVGLGATHAFSPTLLIDWNFGFTRQRLGSTFDLTSARGLNDLHIPGTNNA
                     GATGDPSLYYGWPGFTFPTGGASLGNAQPANPFLFRDQQFVTGANLSWNKGKHGFRGG
                     IEWNHSQINHFQPQGGTFQQPRGAFQFNGNVTALQGTTPTWFNSWADFLLGLPSTTGK
                     ARALFNPTALRWSQWAWYLQDRYQVTPKLTLTYGVRWEFYPFGYSDNGKGLRVLDLAT
                     GKVLLGGYGSIPRDDGMDVGSGQFLPRIGVAWRLSQNTVVRAGYGMSADPYTWHVLRN
                     AYPSVLLDTNTPANTADFIPAASLTGLNSTGLGGGSYSVPTGIVLAPLPDLSSGTIPL
                     PTNISTTTVPNPFHRGYINSYNLSLEQEIQNNFTYTLGYVGTYAVRPVVNMNANASPP
                     GTGSAGGLLSQAYGANYTGTINKLNPFKHSRYDSLQTSLTYRFAGGSNIRAAYTWSKA
                     MDYADNEDLGFLSFPYPDYWSKNYAPAGFDRTNNFEFAGVIALPFGKEGLWAKSGIGG
                     ALLGGWLVNPVLSIMSGAPFTVSAGGNLNANGSGQTADLVKPFRLTHGKPPRTGVTCA
                     LGDTSCEYFDPTSFVAPWICTNPSPSACPAGISPPHYGNTNRNEFRGPGFFNMNLSVV
                     RDFKVREWATLQVRADAFGLTNTPHFANPNTSCPANATIPGQLCNTGTNNNFGVITGT
                     AQPGGFFGPDPGNRTMWLGARVQF"
     gene            9642..11000
                     /locus_tag="VFA02_05705"
     CDS             9642..11000
                     /locus_tag="VFA02_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZU09380.1"
                     /translation="MLYFRRSVFHTMVALYFSTALAQTGQTPPVHLTSEQDHQRMMDL
                     LHITSLRPGVSGNPKAQNAANYDESKAGPFSPLPDPLLLKNGKRVTSAKMWWKQRRPE
                     LVEEFDREIYGRQPANTPKVHWELLSTTHETNGDIPVITKKLAGHVDNSSYPAVSVTI
                     PLTLTIPANAKGPVPVIIEFTFDPAFLAELAKHGIKLPPSPPGPTWQQQILAKGWGYA
                     VLIPTSYQADNGAGLTEGIIGLMNKGQPRKPDDWGTLRAWAWGASRVLDYLETDKSVD
                     AKRVGISGHSRYGKAALVTMAYDPRFSIAYISSSGAGGAKIFRHNFGEPLENVAATNE
                     YHWMAGNFLKYAGPLTVKDLPVDAHELIALCAPRPVFIGVGSAEGDGWADARGMFLAE
                     VAAGPVYQLLGKKGLGTDKFPPMGTALIDGDLGFRQHSEGHTQLPNWPTFLDFASKYW
                     GQ"
     gene            11103..11312
                     /locus_tag="VFA02_05710"
     CDS             11103..11312
                     /locus_tag="VFA02_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZU09381.1"
                     /translation="MPTPKAEKTTKPRKAPARKKTSAPPEPTVIRPTYEQIAQRAHQL
                     WIERGRTHGRHEEDWFRAEQELMAS"
     gene            complement(11369..12385)
                     /locus_tag="VFA02_05715"
     CDS             complement(11369..12385)
                     /locus_tag="VFA02_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HZU09382.1"
                     /translation="MIALLTSLFCAAAPAQQPKALNLSGEYWGTHDPSIIKEGDTWYV
                     FATGKATDGGQFQVRCSRNLTDWKLCGHVFDGIPAWIQKDSPGTKELWAPDISYVNGE
                     YRLYYAYSLFGKNTSGIALATNKTLDPSSPDYKWIDRGLVLKSTAADDFNAIDPNFVR
                     DAQGHAWLAFGSFWSGIKMRRLNDRTGLLSATDTELYSLASRRRPENPPPAKPDLPPD
                     WQAVEAPFIVHHGSYYYLFVSWDLCCRGTHSTYRTMVGRAKSVTGPYLDRNGTLMTEG
                     GGMELLHGNSRWLGPGGESVWMGKDGTCLLVFHAYDAKTGRPALQISTITWEDDWPHV
                     ALQQ"
     gene            complement(12527..12748)
                     /gene="xseB"
                     /locus_tag="VFA02_05720"
     CDS             complement(12527..12748)
                     /gene="xseB"
                     /locus_tag="VFA02_05720"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZU09383.1"
                     /translation="MPGFEESLKRLEKIVEQLERGDLPLEESIRLFEEGTRLSAECKD
                     YLDKAEGKVQILIKQRDGSAKPEPFSPAK"
     gene            complement(12735..14645)
                     /locus_tag="VFA02_05725"
     CDS             complement(12735..14645)
                     /locus_tag="VFA02_05725"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HZU09384.1"
                     /translation="MANRLQDIFANRTVLFDGAMGTMLYARGVFINRCYDELNLSQPE
                     LVRSIHDEYLQAGAEIIETNTFGANSFRLTRYGLQDQVTAINQAGVRIARQAVNQLQE
                     KQAGTAWVAGSVGPLGIHLEPLGKTSLEEARAAFAEQIRALAAGGPGVGADLIVIETM
                     PALNEAEQAILAAREAAPGLPVIVLVTVDEDAHCLDGATAEVAGTKLTAWGADAIGVN
                     CSTGPATVLTAIECMAAVSDLPLVVMPNAGMPRAVDGRNIYLCSPEYMASFARKFIKA
                     GAQFIGGCCGTTPNHVRAMKSAMRAQEAQKAAHAHASRAPIVTETPPPPLEERSRVGR
                     LIHDGKFVTMVEIVPPRGIDCSKELGGATLLAGLGIDVINVPDSPRASARMSAQSLCI
                     QIQQKVGIETILHYTCRDRNVLSIQSDLLGAASIGLKNVLCLTGDPPKLGNYPDATAV
                     FDVDAIGLVNIVNRLNHGLDIGGNPIGASTGFTIACAANPGVPDIDNEVRRFAYKVEA
                     GAEYAITQPVFDLRVLESFLKRIEDFRIPVIAGIWPLTSLRNAEFMKNDLRVSVPDSV
                     MLRMQAAPSPEAARAEGIRIAQEMLEAARTMVQGVQVSAPFGRYASAAEVLAAVLPSE
                     KEPVEGASVARL"
     gene            complement(14737..15447)
                     /locus_tag="VFA02_05730"
     CDS             complement(14737..15447)
                     /locus_tag="VFA02_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZU09385.1"
                     /translation="MPEKKTLHTLCIDIGGTGLKMMVLDENGHPLTERLRTPTPADPS
                     PAKVLAALDQLKKKAPAFDRVSVGFPGVVKRGILYTAANLHPKWVEFNLQAELEKRWK
                     KPVRVANDAAVQGYAAIRGRGVEMVLTLGTGLGSAIYTDGRLCPGLELGHHPWMKGKT
                     YEDFLGKRGLKKHGAKKWNKLLARAIQQTTHTFNWDYLYIGGGNTKFISVPLGENVQV
                     VPNEDGLLGGVALWKHNA"
     gene            complement(15467..16021)
                     /locus_tag="VFA02_05735"
     CDS             complement(15467..16021)
                     /locus_tag="VFA02_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU09386.1"
                     /translation="MKILQKKSAGALITWVLLAGLPATGKSTLARRLAAQLGRAAVLD
                     KDRVREALFPGEMTDYSAEQDALCMKAIIEAATYLTQHNLANYLFFDGRTFSRAAHIE
                     EVLTAAESAGAQWRILHLTCADQIAESRLRAPDSAHPARNRDAALYYRVKASFQPLCR
                     PKLDLDTSAGIEDILPHALAYISA"
     gene            16544..17482
                     /locus_tag="VFA02_05740"
     CDS             16544..17482
                     /locus_tag="VFA02_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09387.1"
                     /translation="MFSGRMLRLLLSALMTALTLTTAYAAPRSNPLLGLVPPGSEIVA
                     GIEDPATDHILLVTASNTYDFSDFLALIGVDAHRRINKMVEVAASSPRGELTERLLLA
                     AGQFDRDHVFHAAVANGATTTNFSGVNVLVVAPFAREKQQMQDTRWMAILDNRVLAFG
                     TPVMVKTAIGRYQTGRGPDEVLAQRIAQLQGDVGSWNVLAMPAAVAARHVAPGQIEPF
                     WMRLMQHGNVLTFGIRDGAKMRLYVNLETASEGEAAGVAESLRSSGVLRANLGQGRGM
                     RVDSVSVEGNRVHAVLSATGKERYAQLLPAAQSLIP"
     gene            17569..>18092
                     /locus_tag="VFA02_05745"
     CDS             17569..>18092
                     /locus_tag="VFA02_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HZU09388.1"
                     /translation="MAKATLNRMVPELLLAASVAIAPAVFAQSGSSDPAQAQTQQPAA
                     TPAQTQQNQVPQQQPGTTPPTIPSGQTDDKANADKENQPQLPPPAQEPKEVHVKKGSI
                     DDVSAVGTRNIGGRGLGNWYSTESEIKMGKSAADEIEKSTKMITDPVVTEYVNRIGQN
                     IVRNSDCKVPFTIKV"
BASE COUNT         3932 a         5038 c         5144 g         3978 t
ORIGIN      
        1 acaccaggtt ttacgctgac tgcgattctc actctggcgc tcggcatcgg cgtaaacgca
       61 gccatgtttt ccgtcatgga ccaggtgctc ctgcggcctt tgccgtaccc caatgcgcaa
      121 aggctggtgc tgatgggggc cgtccggcag cagggtgggt tcgcggctgt gtccttgcca
      181 gacatgaaag actggcaggc gcgcaatcat acctttcagg caatcgggta tttcgagtcg
      241 aacccggtca cattgcgttt tggggattct tccaacgtta cgacccaggt catgaccagc
      301 accaacctct ttgagctgct cggtgttcat ccagctatgg ggcggtcctt tactgctgaa
      361 gacggcaaat cgggaagaag caatatcatc atcgtcaccg accgcatctg gcgcaattat
      421 ctccacagcg acccgaatat tctgggcaag gctgttttca tcaacggcga cccatatacg
      481 gtcatcggcg ttctgcctcc cgccgtgggc tttcccggag atggccttga aagcatttat
      541 gcgcccgtca atccggacga caacggcctg tccgaccggt caagctctgc tctgctggcg
      601 ataggtctgc tacgtcctgg ggtgaccgtt gaacaggccg gccacgaaat caactccatt
      661 caccagcagt tgctccgtga atatccaaaa gacgaatcaa cagcccttat tcgtgtcgtg
      721 aactatcgcg acgcactcac agaaaattcg cggtctgcca tttttgcgct gtacggcgca
      781 atcatcgcag tctggctgat tgcctgcgcg aacgttgccg ggctgatgct tacccgcacc
      841 aacaaccgtc gccgcgaaat cgccattcgc ggcgcgctcg gagcaggcag agacagactt
      901 gtccagcagt ttcttaccga aagcctgctg atttctcttg cgggcggcgc tgcaggagca
      961 ggtttcgccg caattgccct ggggttgtta aaacactatt tcgcgcatac gctgctttat
     1021 ggcgggttca tccatatcaa cggtgcagtc tgcgcctttc tcctgctggc ctcctgtatc
     1081 tcagctttgc tcttcggggt cgttcctgcg tggatggccg ccaatactcc tttgcaggaa
     1141 gggctgcgtg aaggcaccgc ggcagcaggc gtgagcagga agcaggcatt ctggcgcgat
     1201 gcttccgtcg tggcagaaat caccctgact ctggctttgc tgattgccgc aggactcatg
     1261 atgcgcacgc tttaccagtt gcgccgtgca gaaaagggct ttattgccga ccaggttgtg
     1321 actggaatgc tgtttatgcc cacacatggc gcctggtgga cttctcccga tcccgagaaa
     1381 gcagccaacc tggtaaagat gttctatcgt ccgctccagg aaaagctgct gcacactccg
     1441 ggaatttccg gcgttggctt caccaccgta cgcccgcttg agcctggctg ggattttgtt
     1501 gacaacatta ttgtgaatgg aaggccaaag cccaataaat ccgagcaacc cactgcgcaa
     1561 ttgcgcggta cggatgaagg ctatttcagg gtattcggca tccgactgtt gaagggccgc
     1621 tatttcgatt cccaggacac acccgattcg cctgttgttg cagtggtcaa tcagtccttt
     1681 gtaaaagaag tcttccctcg agaagacccg attggtaaac agatagaaat cgccgatgct
     1741 cccggcccgc gtcactgggc gaccattgtg ggtgttgcag aggatgtccg gcagcgctcc
     1801 atgggagaag ctcccgtccc ggagctggac ctgaacctga tgcagttcag cccaaaggac
     1861 gagatgtatc ccatcatctc gtctttcatg aacatcgctg tgcgcacaaa ccttcccgcg
     1921 gccgattccg agcgcatcat cgccaacgcg atccacgacg tgatgcctga cattgctttg
     1981 caaaacttca agcccatgcc gcaggtggta gacgattcca tgggcaacca gacccttgcc
     2041 gcacgcctgc tcggcatctt tggcatcgcc gcgctggcca ttgccattgc aggaatctac
     2101 ggactacttt cttattcggt cagccagcgt atgcgcgaat tcggagtgcg tctggcactg
     2161 ggctctccgc gaggcgccgt catctggctg gtactgcgcc atgctctgct gctgcttgcg
     2221 gttggtattg ctgccggaat caccattgcc attgccgcca gcggagtgat gcgcgccttt
     2281 atttatggtt tccacggcta tgacgtcctc actgtcttcg ccgtcgccct ggtgcttgcc
     2341 gcctgcgcac tgctggccag ctatctcccg gcccgcaagg ccgccagggt agacccggtg
     2401 gtcgctctgc gcaccgaata ggaagagtag cgcagtgctt acggcatttt cattggatct
     2461 accgcaaaaa cagcagacgg ctttcaccag tctgtgagtg tcccatccag ctttcgggca
     2521 atcggcagat acgccctctg atacggatat ttcgtagcca gtttttcatc cagttccacc
     2581 cccagaccag gagcatcccc cggatacatg tatccggaat cgaagcggta cccatgcgga
     2641 aaaacagcat cggtttccgt cgagtgcggc atgtgctcct gaataccgaa gttgtggacg
     2701 gctatgccga agtgcagcgc agcggccatc gtcaccggag aaagatcggt cgccccgtgg
     2761 catcccgtgc gaatgtggta gagtgacgca aaatcggcaa cttttttcag atgggtcagc
     2821 cctccggcat gtatcactga catgcggatg taatcaatcc attggttgcg aatcaaatca
     2881 tgcgcgtccc agatggtatt gaagacctcg ccagtagcaa tgggagtagt tgtgtgctcg
     2941 cggataattt tgaagccttc ctgcaactcg gcaggcacag ggtcctccat ccagaagagg
     3001 cgaaacggct ctagctcttt gccaagacgt gctgcttcga ttggtgtcaa acgatgatgg
     3061 acatcgtgca aaagatgaac atcctcgccc actgccatcc gcagcttctc aaacagcctg
     3121 ggagtatgcc gcaagtatag ttccgtagac cagcggcttt ccgaaggcag tccgcgctcg
     3181 gccggctcat agggctgcgc gcctttcgga acaccgtaag tcgaggaaag tccgggaatg
     3241 ccgctctgcg cgcgcactgc gcgataaccc atctccatgt gcttctgtac gtcggcgatg
     3301 gcttcttcta ccgtagcgcc atgcgcatgt gcatagacca acactccttc gcggctcttg
     3361 ccgccaagca ggttgtaaac aggagttccc agcgttttgc ctttgatgtc ccacagcgcg
     3421 acatctacag cggcgatagc agccatcgtg accggcccac gccgccaata cgctccacga
     3481 tagagatact gccagatgtc ttcggtctga aacggatcgc gtccgataat acagggaaca
     3541 acgtgctcgg taagatagct gacaaccgcc agttcgcggc cattcagagt gccatcaccg
     3601 aggccgtaaa tgccttcatc ggtctcaatt ttcagcgtga cgaagttgcg tcccggtgag
     3661 cagatgttga cagaagcctt cgtgatcttc attggacaga atgcctttgc agaagcagtg
     3721 ccgcagcgcc gcgcagacgg gccacatcgc cttcgtgtgt gggcagaagc cgcggcgggc
     3781 ctccggcaag aacaacttct ttgagttcag cctcaatcag cggagcgaaa cgtccccagg
     3841 cagaggtcac atctcctgcg acgagtacca cttccggcga aagtccggca accactgtgc
     3901 ggagtccttt gcccagaaac ctagcttgct ttgccagcgc tgccgcagcc tgggtatttc
     3961 cttcttcggc cagacttaaa agctcatgga aggtcatgcg ctttgacttt ggctcaagct
     4021 cgctgtagta gcgcagggcg gcgttgcagg aagcgaacat ctcccagcac cctgtgccct
     4081 tgcaggcgca acgcgggccg gaagggtcaa gcgtgacgtg gccgaactct ccagccattc
     4141 catttcttcc cgagatgatc tgcccgtttg ccatcagccc ggagccaata ccctctgaaa
     4201 ccgtaatcag cacagcatcg cggatgccgt ccattctgcc gaaccataat tctgagagca
     4261 ggcaggcggt tgcagcattt tccatctctg tgtgcagacg catttcacgc tcaaaggcac
     4321 cttttatgtc aaaatccggc cagtgaagat tgggggcgaa gaccagacgc tgcgtttcgg
     4381 ggtctacgcg accgggcagg ctgatgccaa tgccttcgac agatttcctc ggatacgcat
     4441 cgcgaatgcg cttcatgcac tcggtcatgc cgtggacggc cttcgccgga tcttttccaa
     4501 tcggcaacgt ggaacgcgag agcaggcggc cattgagatc tacaagcgcc accgtggcct
     4561 ggtgcggatg aatatctgct gccagcacca caaggtcatc attcagatga acaagcgtag
     4621 gacgccgccc acgaggcagg tgggccagcg cgccttcgcg cacccacttt tcttccagca
     4681 gttgttcaat gatctgcgaa acggtgctgc gttggaggcc ggagagacgt gccagatcgg
     4741 cccgcgaaat aggctggcgc gtacgaatga tttgcagaat aatgtcgcgg ttgatgcggc
     4801 gcgctgtctc actcgatgcc agttcggcag aggtgaggtc cacatgccgc attccttttc
     4861 ctgtttgtct cgtccgtccc aagtctttcc ttgtctgatg tccaatgtac accggaatgc
     4921 ttccggttta ctggttcact ccgctggcca gatagcctcc atctacaaca atgatctgcc
     4981 cattcacata agaagcagca tcggaggcaa gaaagaccgc cgcaccaacg agttcatccg
     5041 tgtgcccgaa ccggtgcatg gggatgcgca tgtgcatttc gcgtccgcgc ggagtactgt
     5101 ccagaaggtc ggcattcagt gatgtaggaa aaattcccgg cgcaagggca ttcacgcata
     5161 tgccgtcgtg cgccagctcc acggcaagcg aacgggtgag ggaagcaacg gccgctttgc
     5221 tcgccccata agcggcgact tcatgaaaag caacaaacgt ggccagagaa gcgatattga
     5281 tgatccggcc atagcgctgc gcctgcatgg tgcgtccgaa aatctggcac gaacgaaatg
     5341 tacccgtaag gttggtttcc aggatgtaat tccagtcttt ttcagagcac tcaagcgtag
     5401 gcacgcgctg tgtggtcccg gcagaattca cgagaatgtc cacgcgcccg aatttttcta
     5461 ggactttgtc atgcaatgac tgcagagatg cccggtcgag cacgtccgac gctactgcca
     5521 gactccggca gcccagccct tcaatttcag ctgctgtctg ttttacttct tccaggcgac
     5581 gcgaagaggc cacgacatct gcccccgctg cggccaaccc taaagcaatg gagcggccaa
     5641 tcccggaagt tccgccgata ataacggctg tttttcccgc cagattaaaa atgcattgac
     5701 tcaaatggtc cccgctggat ctgttgatag tggatttaat ttgtattcaa tcgaaaaatc
     5761 tagtgtaaag ccctgtcctc gcctgctgga ataaaaaact gctctacgcg aaaaacgatc
     5821 atgcaaatta aataatcatc tccacccctt ttcaaacgat aaaaattacg gttgacaatt
     5881 atcattgctg ctcaatatag tttgcgtcgc gtactaaatt gatttaaccg agagcctttc
     5941 gaggacaggt acgcgctcga gtcaaaaatt ttggaggcag tcatgatgag ggttcgcttt
     6001 aggggtcttt gggtctgtaa tcttctgctt ttgctttact tgctgttgag cagtgcggtc
     6061 tacgcgcagg tgctttatgg ctcgttgaca ggcacggtca ccgacagcac gggcgcggtc
     6121 ctggaaggca cccaggtaac agttctggat gtccagactg gaacgacgca atctgcaacg
     6181 acggattcca gcggcatcta ccggtttaca accctcttgc cgggaacata caaggtgaca
     6241 attgcggcgt ctggttttac ttcacaggag accgctgggg tcatggtgcg cgccaatgag
     6301 atcgcccgtg tggatgcaca actgaaggtg gcaacagcaa cgcaaagtat cactgtgacc
     6361 actgcgcctc cccttcttca gaccgacaag gcggacgtgc acaccgatct tacttcgcgt
     6421 gagattgaaa acctgcccat catggggtcg cagggggcga attttcagtc tctgctgcgg
     6481 acgatcccgg gcgcagggtt gacggcggag accaactctc ttgccgggaa tccgcagcga
     6541 gccatcaaca cgaacatgaa tgggctttca aatcagggaa acaacacgcg cattgatggg
     6601 gcacaagatg cctatccctg gctgcctgcc aacgtggctt atgtaccacc tgcggatgcc
     6661 attgaatcag tcaacgtggt gaccaactcc tttgatgccg agcagggaat ggctggcggg
     6721 gccgccatca acgtgcagat caagtcaggt acaaacaagt tccacggcag cgcgcacgaa
     6781 tatcatacgg accagaactt tgctgcgcgc aattatttcc agaccgaccc caaaatcttc
     6841 ccccacaaaa acagaaacaa tcaaaaccag tttggaggga ccatcggtgg tccagttgtg
     6901 aaagataagc tgttcttctt tgccgattac gagcgcacga cacaacggca aaaggccgga
     6961 ccggatacgc gcacccttcc caccgcagcc atgattgcgg gcgatttccg aggtctgccg
     7021 ggaagcccca taatctatga tccggcaacg ggcgaccagc acggggccaa caagcagcag
     7081 gtctcgtgca atggggtacc caacgttatt tgcccaaacc ggattgatcc tgcggcacag
     7141 gccatggtca aacttttgca gccattggtt tcgcaggaat tccagacaag caatggaatg
     7201 aacaactgga ccggaagtgg aactgctttg tttaaccgcg acaatgctga cttcaaggtc
     7261 acctacgttc caaaccagaa ctccacggtt tttgggcgct atagcttttc aaagaccctg
     7321 gtttttgacc cgcctcttct tggcgctgca ataggggacg ccaccaacgg aggccagctt
     7381 gggaatgctc ccggcctggt acagagcgtt ggtcttggtg caactcacgc gttcagtccc
     7441 actctgctga ttgactggaa ctttggtttc acgcgccagc ggctggggtc cacattcgat
     7501 ctgacctcag caagggggct gaatgacttg catattcccg ggaccaataa tgccggtgcg
     7561 acgggggatc cttcgcttta ttatgggtgg ccgggtttca catttccaac cggaggggcc
     7621 agcctcggta acgcgcagcc tgccaatcca tttctgtttc gtgatcagca gtttgtgact
     7681 ggagccaacc tgagctggaa caaaggcaag catggtttcc gcggcggaat tgaatggaac
     7741 cattcgcaga tcaaccattt ccagccacaa ggtggaacat tccagcaacc tcgcggcgca
     7801 ttccaattca atggcaatgt cactgctttg caggggacca cgccaacctg gtttaactcc
     7861 tgggctgact tccttcttgg attgcccagc acgacaggaa aagcccgggc actttttaac
     7921 cccactgctc tgcgttggtc acaatgggcc tggtatctgc aggaccggta tcaggtgact
     7981 ccgaaactga cgctgaccta cggagtacgt tgggagtttt atcctttcgg atacagcgat
     8041 aacggaaaag ggctccgcgt cctcgatctg gccaccggca aggtccttct cgggggatat
     8101 ggaagcattc cccgggatga cggcatggat gttggctcag gtcagtttct gccgcgcata
     8161 ggagttgcct ggcgcttatc tcagaacact gttgtccgtg ctggttatgg aatgagcgcc
     8221 gatccgtaca cctggcatgt gttgcgcaac gcttatccgt ctgtgctgct ggatacaaat
     8281 accccagcaa atacggccga ttttattcct gcggccagtc tgactgggct gaatagcaca
     8341 ggtctgggag gcggaagcta ttccgtgcct accggcattg ttcttgcgcc attgcctgac
     8401 ctgagtagtg gtaccattcc actgcccacc aatatcagca ccacaaccgt tcccaacccc
     8461 ttccatcgtg ggtatatcaa ctcctataac ctctcactgg agcaggagat acaaaacaat
     8521 ttcacgtaca cccttggcta tgttggcact tacgctgtcc ggccagtagt gaacatgaat
     8581 gcaaatgctt caccgccggg taccggcagt gccggcggtc tgctgagtca ggcatatggc
     8641 gctaactata cgggaacaat caacaagctg aatcccttca aacatagccg ctatgattca
     8701 ttgcagacca gcctgactta ccggtttgct ggcggttcga acatacgtgc ggcctatacg
     8761 tggtcaaaag caatggacta tgcggacaat gaggacctgg gctttctttc cttcccctat
     8821 ccggactact ggagcaaaaa ctatgcgcct gctggtttcg accggaccaa taactttgag
     8881 tttgcaggcg taatagccct gccttttgga aaggaaggac tgtgggcaaa gagcggcatt
     8941 ggaggtgcac ttcttggagg ctggctcgtc aaccccgtgc tgagcatcat gagcggtgcg
     9001 ccatttaccg tcagtgcggg cggcaatctg aatgcaaatg gctccggtca gacggctgat
     9061 ctcgtcaagc catttcgtct gacgcatgga aagccgcctc gcaccggggt gacctgtgcg
     9121 ttgggtgata catcctgcga atattttgat ccaacctcct ttgtggcccc atggatttgt
     9181 acgaacccca gcccgagcgc gtgcccggca ggaatttcac ctccgcatta cggtaatacc
     9241 aatcgaaatg agtttcgcgg cccaggcttc ttcaacatga acctgagtgt ggtacgggac
     9301 ttcaaggtca gagagtgggc cacattgcag gtaagggcag atgcttttgg gctgacaaat
     9361 actccccatt ttgccaatcc gaatacttcc tgcccggcca atgcgaccat accaggccaa
     9421 ttgtgcaaca cgggcacgaa caacaacttt ggcgtcatta ctggaactgc gcagccgggt
     9481 ggtttctttg gccctgatcc tggtaaccgg acaatgtggc tcggcgcccg tgtgcagttt
     9541 taaacagcca ccaatttgct tccgtggggg aggctttctt agctgaaagc ctcccttctt
     9601 tttcagttat ctttatcttt tgcaaaagag gagaatctcc gttgctatat ttccggcgat
     9661 ctgtttttca tacgatggtg gcgctgtatt tctcaacagc acttgctcaa accggacaaa
     9721 ctcctcctgt gcatctgacc tccgagcagg accatcagcg catgatggac ctgctgcaca
     9781 tcacttctct gcgtccggga gtgagcggca atcccaaagc tcaaaatgct gcgaattatg
     9841 acgaatcgaa ggcagggccg ttttctccgc tgcctgaccc gctccttttg aaaaatggga
     9901 agcgcgtgac ctcagcgaaa atgtggtgga agcagcgccg tccggagctg gtggaagagt
     9961 tcgaccgcga aatctatggg cgccagcctg caaacacgcc aaaggtgcac tgggagcttt
    10021 taagtacaac acatgagacg aatggcgaca ttccggtcat tacgaaaaaa ctcgccgggc
    10081 atgtggataa ttcctcgtat ccagcagtga gtgtcaccat cccgcttacg ctgaccattc
    10141 cggcgaatgc gaaaggaccg gtccctgtca tcatcgagtt cacctttgat cccgcctttc
    10201 ttgccgagct ggccaagcat ggcatcaaac tgcctccttc gccaccaggg ccaacatggc
    10261 agcaacagat tcttgccaaa ggctggggat acgcggttct cattccaacc agctatcagg
    10321 ccgataacgg agcagggctg accgaaggca taatcggcct gatgaacaaa ggacaaccgc
    10381 gcaagccaga tgactgggga actctgcgcg catgggcctg gggagcaagc cgcgtccttg
    10441 attatctgga aacggacaag tccgtggacg cgaagcgcgt tggtatcagc gggcattcac
    10501 gctatggcaa ggccgccctg gttacgatgg cctacgatcc gcgattttct atcgcttata
    10561 tcagctcatc cggagcaggc ggagcgaaga ttttccggca caacttcggt gaaccgctcg
    10621 aaaatgtcgc ggccacaaat gaataccact ggatggccgg aaactttctg aaatatgccg
    10681 gccctctgac ggtgaaggac cttcctgtag atgcccatga actgattgcg ttgtgtgcgc
    10741 cgcgcccggt atttatcggg gttgggtctg ccgaaggcga tggatgggcc gacgcgcgcg
    10801 gaatgttttt ggccgaggtg gccgcaggac cggtctatca gttgctgggc aaaaaggggt
    10861 taggaacgga caaatttccg ccgatgggca cagcattgat tgacggcgac ctggggtttc
    10921 gccagcatag cgaagggcac actcagcttc cgaactggcc aacatttctg gacttcgcca
    10981 gtaaatactg gggccagtaa ggttcctact tcatcaatca caggaacaga agatgttttc
    11041 agagcaaggc agtgcctcgt atcatagaag aaactactct tgaggaggaa ctcattaccc
    11101 ccatgcctac tccgaaagct gagaagacca cgaaaccccg caaggccccg gccaggaaaa
    11161 agacctctgc gccgccggag ccaacggtca tccgtcccac ctatgaacag attgcgcagc
    11221 gtgcccacca gctctggatt gagcgcggac gcactcacgg caggcacgaa gaagattggt
    11281 ttcgcgccga acaggaactt atggcgtcgt agtctctgcc atcgttctca ttcttaatct
    11341 tagtggcgct gcggcttgca gcgcttttct attgctgcaa agcaacgtgc ggccaatcat
    11401 cttcccaggt gatggtggaa atctgcagcg ccggcctgcc tgtctttgcg tcataggcat
    11461 gaaacaccag aaggcaggtg ccatctttgc ccatccagac agattcgcct ccagggccaa
    11521 gccagcgcga attgccgtgg agcagttcca tgccgccgcc ttcagtcatg agagtgccat
    11581 ttcgatcgag gtaggggccg gtcacgcttt tggcgcgccc aaccatcgtc ctgtaggtac
    11641 tgtgggtgcc acggcagcaa aggtcccagg aaacaaacag ataataataa ctgccatgat
    11701 ggacgatgaa aggcgcttcc acggcctgcc agtccggagg aaggtctggc tttgcaggag
    11761 gcggattttc cggcctacgg cgggaggcca aagagtagag ctctgtgtcc gtggcggaaa
    11821 gcaatccggt tctgtcattc aggcggcgca ttttgatgcc gctccagaag ctgccgaagg
    11881 caagccacgc atgcccctgg gcatcgcgaa caaaattggg atcaatggcg ttgaagtcat
    11941 cggcggcagt ggacttcagc accagtccac ggtctatcca cttgtaatcc gggctggatg
    12001 ggtcgagggt cttgttcgtt gcgagcgcaa tgcctgacgt attttttcca aagagtgaat
    12061 aggcgtaata gaggcggtac tcgccattta cgtatgagat gtctggcgcc caaagttcct
    12121 ttgtgcctgg actgtctttc tgtatccagg caggaatccc gtcaaagaca tggccacaga
    12181 gcttccagtc ggtcaggttt ctggaacagc ggacctggaa ctggccacca tctgtggcct
    12241 ttccggtggc gaagacgtac caggtatcac cttctttgat gatggatggg tcgtgcgtgc
    12301 cccagtattc accggacagg ttgagtgcct tcggttgctg ggcaggtgcg gcagcgcaaa
    12361 acagggacgt caggagggca atcatgtatt tgcgaaacat atattgagaa accgttttct
    12421 ctgcatggag agtatcacac gcttcaaacc agagactttg cggcaaacgg caccacggcg
    12481 aaactgcatc tggatgccct ttttgcagag atggacggga accggactat ttagccgggg
    12541 aaaacggttc gggtttggca gagccgtcgc gttgttttat cagaatctgc actttgcctt
    12601 cggccttatc aagatagtcc ttgcattcgg cggaaagacg cgtgccttcc tcaaacaacc
    12661 ggatggattc ttcgagcgga aggtcgccgc gctccagttg ctcgacaatc ttctccagac
    12721 gcttcagtga ttcttcaaag cctggcaacg gatgctcctt cgactggttc tttctcagac
    12781 ggcaggacgg cggccaatac ctcggcggcg gaagcataac ggccaaaagg cgcgctcacc
    12841 tgtacaccct gcaccattgt ccgcgccgcc tcaagcatct cctgcgcaat acgaatgcct
    12901 tcggcacggg ctgcttccgg agaaggagcg gcctgcatcc gcagcatgac ggaatccggc
    12961 acagagaccc ggaggtcgtt cttcatgaac tcggcattcc gcaggctggt cagcggccag
    13021 atgcccgcaa tcaccggaat gcggaaatct tcgatgcgtt tcagaaatga ttccagcacg
    13081 cgcagatcaa atacgggctg cgtaatagca tattctgcgc ccgcttccac tttgtaggca
    13141 aaacggcgga cttcgttatc tatatccgga actcccgggt tggcagcgca ggcaatcgtg
    13201 aatcccgtag atgcgccaat ggggttgccg ccaatgtcca gtccgtgatt caggcggttc
    13261 acgatgttga caaggccaat ggcgtccacg tcgaaaacag cagtcgcatc gggataattg
    13321 cccagcttgg gcgggtctcc ggtgaggcaa aggacatttt tcaggccaat ggatgccgcg
    13381 ccgagcaggt cgctctgaat gctcaggacg ttgcggtcgc ggcaggtgta gtgcaggatg
    13441 gtctcaatgc cgaccttctg ctgaatctga atacagaggc tctgtgcgct catgcgggcc
    13501 gaggcgcgcg gggaatccgg cacgttgatg acatctatgc cgagacctgc gagcagagtt
    13561 gctccgccca gttcttttga gcaatcaatg cctctgggcg ggacgatctc gaccatcgtc
    13621 acgaatttgc cgtcatgaat caacctgccc acgcgtgagc gttcttccag tggcggcgga
    13681 ggcgtttctg tcacgatcgg cgcgcgcgat gcatgcgcgt gggcggcctt ctgtgcttcc
    13741 tgcgcgcgca tggccgactt catggcccgg acatggttcg gcgtagtgcc gcaacagccg
    13801 ccgatgaact gcgctccggc cttgatgaat ttccgcgcga agctcgccat gtattcaggc
    13861 gagcagagat aaatattgcg tccgtccacg gcgcgcggca ttccggcatt gggcatgacg
    13921 accaggggaa gatcgctcac tgcggccatg cactcgatgg cggtcagcac ggttgcaggc
    13981 ccggtgctac agttcactcc gatagcgtcc gcgccccatg cagtcagttt ggtgccggcc
    14041 acttcggcgg ttgctccgtc gaggcagtgc gcatcttcat ctacggtgac cagtacgatc
    14101 acgggcaggc caggagcggc ctcgcgcgcg gccagaatcg cctgctctgc ttcattcagc
    14161 gcgggcatgg tttcaatcac aatcaagtcg gcgcccactc cggggccgcc cgcggccagg
    14221 gcgcgaatct gttctgcaaa agcggcgcgg gcttcttcca gagaggtttt gcccagcggt
    14281 tccagatgaa tgcccagagg accgactgac ccggcgaccc aggcagttcc ggcctgtttc
    14341 tcctggagtt gattcacggc ctgccgcgca atgcgaacac ctgcctgatt gatggccgtg
    14401 acctggtcct gaagaccata gcgagtaagc cggaagctgt ttgcgccgaa ggtgttggtc
    14461 tcgatgatct ctgcgcccgc ctggaggtac tcgtcgtgga tggagcgcac gagttccggc
    14521 tgggaaaggt tcagctcgtc atagcagcgg ttgataaaga cgccgcgcgc gtagagcatg
    14581 gtgcccatcg cgccatcgaa cagcacggtg cggttcgcaa aaatgtcctg cagacggttt
    14641 gccatttact tcctgtgaga tcgttggtcc tcgcctttcg gggcaaggac caggggcctt
    14701 ctagtcatgc taagacactg gctgcgggaa agccagtcat gcgttgtgct tccatagggc
    14761 aacgccgccg agcaaaccgt cctcattggg gaccacctgt acattttcgc ccagcgggac
    14821 actgatgaat ttggtgttac ctcctccgat atagaggtag tcccagttaa aggtatgtgt
    14881 agtctgctgg atggccctgg ccaaaagctt gttccatttc tttgcgccgt gctttttcag
    14941 gccgcgtttg cccagaaagt cttcataggt cttgcctttc atccagggat ggtggccaag
    15001 ttcaagcccg gggcaaagcc tgccgtctgt gtaaatcgca gagccgagtc ccgttcccag
    15061 agtgaggacc atctcaacgc cgcgtccgcg aatggccgcg tagccctgca cagcggcatc
    15121 attcgctacg cggacgggct ttttccagcg tttttccaat tcagcctgaa gattgaactc
    15181 cacccatttg ggatgaaggt tggcggccgt atacaagatg ccgcgcttta ccactccggg
    15241 aaaaccgacc gaaacgcggt cgaaggcggg ggccttcttt tttagctggt caagggcggc
    15301 aagcactttt gccggggacg ggtcagcagg cgttggcgtg cgcaggcgtt cggtcagcgg
    15361 atgcccgttc tcgtcgagca ccatcatttt caggcctgtt ccgccaatgt caatgcagag
    15421 ggtgtgcagc gtcttcttct ctggcatacg aaaggaatat acatctttac gctgagatgt
    15481 acgcaagcgc gtggggcaga atgtcttcga tcccggctga ggtgtcgaga tcgagctttg
    15541 gacggcagag cggctggaag gacgccttga cgcgatagta aagggcggca tcccgattac
    15601 gggccggatg ggcagaatca ggcgcgcgca ggcgagactc ggcgatctgg tcggcacagg
    15661 taagatgcag gatgcgccat tgcgctccgg cgctttcggc agcagtaagg acttcttcga
    15721 tgtgcgcggc gcggctgaag gtgcgcccgt cgaagaagag atagttggcc aggttgtgct
    15781 gcgtaagata agtggccgct tcgatgatgg ccttcatgca cagggcgtcc tgttcagcgg
    15841 agtagtcggt catttctccg ggaaagagcg cttcgcggac gcggtctttg tcgaggaccg
    15901 cggcgcgacc aagctgggca gcgagcctgc gggcgagagt gctttttccg gtggcgggga
    15961 gtccggcgag gaggacccag gtgatgagag cacccgctga ctttttctgt aaaatcttca
    16021 taaatcagga gttaagcgga aggccgaagc agtgcatttt tctaaattcg tcaaaaagaa
    16081 agacttacgg atgcgttgct ggcaaaatct tcaaaacaaa agaattacgc gttcaactct
    16141 gctcatgaag gcgctcttat tgcttggccc cctattttca gtatagccga tgcaatgtag
    16201 gcttttggaa catattcgtt caaacgaatg atttaatgca tccgaactcc tgctggtttt
    16261 catcgcagga cagtatcacg atgacgcagg cggcggggcg ggtcaaggga agggtcaagg
    16321 gaaatttgca cccacagcgg ggccgcatcc tttcgcgcgg cctttgcgca agagggcagg
    16381 cctattccgt aaagattccg tctggtttgc gttgtgaaga aaccgtgggc gtggagaatg
    16441 tgtccatttc gcgtcagacc gtaaacaaat ctctgggccg cccgtagttg cagacaggaa
    16501 ggacttcggt ctgaacctgt ttccggcaat ggaggctgag aatatgtttt ctggacgaat
    16561 gctccggctt ctgctgtcag cgcttatgac agctttgaca ttgaccaccg cgtatgccgc
    16621 gccgcggagt aacccgcttc tggggctggt accgcctgga tcggagattg tggcgggaat
    16681 tgaagacccg gcgactgacc atattctgct ggtcacagcc agcaatacat atgatttttc
    16741 tgattttctc gccctgattg gggtagacgc gcaccggcga atcaacaaga tggtggaagt
    16801 tgccgcgtca tcgccgagag gggaactgac ggaacggttg ttgctggccg ccgggcagtt
    16861 tgatcgcgac catgtgtttc atgccgctgt ggcgaacggg gccacgacca cgaacttcag
    16921 cggcgtgaat gttctggtag ttgcgccttt tgcccgcgaa aagcaacaga tgcaggacac
    16981 gcgctggatg gcgattcttg acaaccgggt cctggcgttt ggcactcctg tgatggtgaa
    17041 gacagcaatt ggacgctatc agacagggcg ggggccggat gaggtgctgg cgcagaggat
    17101 tgcgcagttg cagggagatg tcggttcctg gaatgttctg gccatgccgg cagcggtggc
    17161 agcgaggcac gttgctcccg ggcagataga gcctttctgg atgcggttga tgcagcacgg
    17221 gaacgttttg acattcggga ttcgagatgg ggcaaaaatg cggctctatg tgaatcttga
    17281 gacggccagc gagggcgaag cagctggtgt ggctgaatca ctccggtctt cgggtgtttt
    17341 gcgggcgaat ctgggccagg gccgcggaat gcgggtggac agcgtgtctg tggagggaaa
    17401 ccgcgtgcat gctgttcttt cggccacagg aaaagaacgg tatgcgcaac tgcttcccgc
    17461 ggcgcagtct ttgattccat gaaaggaagc aaatatcgag ccggtgggga aagcaggagc
    17521 agacaaaaag gaggatacta tcgtctatag acacaggagt gtgcttttat ggccaaggcg
    17581 actctcaatc gaatggttcc ggaattgctg ctggctgcca gtgtggcgat tgcgccagca
    17641 gtttttgcgc agagcggcag ctctgatccg gcacaggcac agacccagca gcctgcggcc
    17701 actccggcac agacacagca gaaccaggta ccgcagcaac agcccgggac cacacctccg
    17761 acgattccgt cggggcagac agacgataag gccaacgcag acaaggagaa ccagccgcag
    17821 ttgcccccgc cggcgcagga gccaaaggaa gtccacgtaa aaaagggaag cattgacgat
    17881 gtgagcgcgg tggggacgcg caacatcggt ggtcgcggtt tgggaaactg gtactcgact
    17941 gagtctgaga tcaagatggg caagtcggcg gccgacgaga ttgagaagag cacaaaaatg
    18001 atcacagacc cggtggtgac cgagtatgtc aaccgcatcg ggcagaacat tgtgcgcaac
    18061 tctgactgca aggtgccctt caccatcaag gt
//
LOCUS       DASRBV010000150         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_516055, whole genome shotgun sequence.
ACCESSION   DASRBV010000150 DASRBV010000000
VERSION     DASRBV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516055"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFA02_05750"
     CDS             complement(<1..303)
                     /locus_tag="VFA02_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09389.1"
                     /translation="MIEVRGLTKTIRNGTRTVEILRGIDLTVPRGQFIAIMGASGSGK
                     STLLGLMAGLDAPTEGEVLIDGTEISRLPEDKLAQVRGKKIGFVFQSYQLIPTLTAL"
     gene            328..960
                     /locus_tag="VFA02_05755"
     CDS             328..960
                     /locus_tag="VFA02_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZU09390.1"
                     /translation="MDVTRRYFLLFLFTLFAACVFPRAGAADAPVIACFGDSLTAGYG
                     VEPNHTYPDDLQKLLDEHGYHYRVVNKGISGNTSKDGADRLKDVLALHPRIVVLEFGG
                     NDGLRGLPLSATKQNLEEMITTLQKAGIKVLLAGITLPPNYGPDYIQQFNGIYASLAK
                     KYNTPLVPFLLKDVYSVPGSMQEDGIHPTAQGCRQVAANVFEALKPLLRK"
     gene            complement(1033..1563)
                     /locus_tag="VFA02_05760"
     CDS             complement(1033..1563)
                     /locus_tag="VFA02_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU09391.1"
                     /translation="MSDSGKVAPAQQPEPWLRGTLTDVPPVARAVLHALELAKEDVHR
                     WCGPLTDADFDARPLELPSLAFHLRHIVRSCDRLLTYAEGNQLSGEQIAVLKTEMDAG
                     AGREALFAEFEKGLEDAVNRVRVLGAGDLSQPRFVGKKQLPTTVGGLLVHVADHTQRH
                     VGQAVTTAKILLAQKA"
     gene            complement(1563..2402)
                     /locus_tag="VFA02_05765"
     CDS             complement(1563..2402)
                     /locus_tag="VFA02_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZU09392.1"
                     /translation="MLVSCGPPRPDYPVIGAKNFTEQVILGELLAQHIEAKTGMKVER
                     RFYLAGSYICHQALISGRIDAYVEYTGTALTAILKRPVERDPQTVLNSVRNLYQQRYQ
                     ITVMPPLGFENTFAMVVRGEDAQRLHLNTLSDAARYTPQWRLGVGYEFEQRPDGLPGL
                     SKVYGLHFKEPPRTMDLGLLYRALHAHQVDMIAANSTDGQIAEFGLKVLEDDRHYFPP
                     YEAVPLVRDDALKRWPALRSALGDLANKISADEMRAMNEAVDVEHRDPAEVVREFRQK
                     KGL"
     gene            complement(2504..3145)
                     /locus_tag="VFA02_05770"
     CDS             complement(2504..3145)
                     /locus_tag="VFA02_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09393.1"
                     /translation="MSNFLPQYGAEIRTLTLEHLWLTGAAMLFASLIGIPVGVWLTRH
                     ERWARPILGFVNVIQTIPSLAMFGFLLPLPWLGERAARIAIVALTGYALLPIIRNTYA
                     GIRGIDPTIRDVACALGMTEMQLLTKVELPLATGVILAGLRTATVTCVGIATIAAAVG
                     AGGLGEFIFRGVASVDNRLVLAGAIPAALLALGADGLLGLVEKWARVPGAGKP"
     gene            complement(3142..>3684)
                     /locus_tag="VFA02_05775"
     CDS             complement(3142..>3684)
                     /locus_tag="VFA02_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09394.1"
                     /translation="NDIAHQDAVLLRRGIGYVIQETGLFPHFTVERNAGLVPELNGNH
                     ADKGRIHELLKLVGLEPEKFAQRWPHQLSGGQRQRVGLARALAADPAILLMDEPFGAL
                     DPLTRAEMQEMLRDLLQRLKKTVLLVTHDLDEALFLASRIVLLEEGRVVADLPAEDFL
                     GSDVPAVREYAQAFHRGVAR"
BASE COUNT          824 a         1178 c          938 g          744 t
ORIGIN      
        1 aagcgccgtc agcgtaggaa tcagttggta cgactgaaag acaaaaccga tcttctttcc
       61 gcgcacctgt gcgagtttgt cctccggtaa acggctgatt tccgtgccat caatcaacac
      121 ttcgccttcc gtgggagcat ccagtccggc catcagaccc agcaaggtac tcttgccgct
      181 gcccgatgcg cccatgatgg cgatgaactg tccgcgcgga acggtcaggt caatgcccct
      241 cagaatctct actgtgcgcg tgccgttgcg gatggtcttg gtcaaccctc ggacttcaat
      301 cattcagaac acctggctgc ttacaatatg gatgtgacac gacggtattt tctcctcttt
      361 ctttttacgc ttttcgccgc ctgcgtgttc cctcgcgccg gggccgctga tgctcccgtc
      421 atcgcctgct tcggcgactc gctcactgcc ggatatgggg tcgagccgaa ccatacgtat
      481 cctgacgacc tgcaaaagtt gctggacgag cacggatatc actaccgcgt cgtgaacaag
      541 ggcattagcg gcaatacttc aaaagacggc gcagaccgcc tgaaagacgt tctggcgctg
      601 catccgcgga tcgtcgtgct ggaatttggc ggaaacgatg gcctgcgcgg tctgccgctt
      661 tccgctacaa agcaaaatct tgaagaaatg atcactacac ttcagaaggc cggaataaag
      721 gtccttttgg ccggaattac cctgcctcca aattacggcc ccgactacat ccagcagttc
      781 aacggcatct acgcgtcgct tgcgaagaaa tacaatacgc cgctggtccc atttctcctg
      841 aaagacgttt acagtgtccc cggctcgatg caggaagacg gcattcatcc taccgcgcag
      901 ggatgcaggc aggtcgcggc gaatgtgttc gaagcgttga agccgctgct gagaaagtag
      961 ccactacatt ctctgaattc ttcgtcttca tcgcatggct cagggtcaga atgacacccg
     1021 ttgcccgatg ccttacgcct tctgcgccag cagaatcttc gccgtcgtca ccgcctgccc
     1081 aacatgccgc tgcgtgtggt cggccacgtg cacgagcagt ccgccgacag tcgtgggaag
     1141 ctgcttcttc cccacaaaac gcggctgtga aagatcgcct gcgcccagca cacgcacgcg
     1201 attcacagca tcttcgaggc ccttctcaaa ttcggcgaag agcgcttccc tgcctgctcc
     1261 ggcatccatt tcagtcttaa ggaccgcaat ttgttcaccg gaaagctggt tgccttccgc
     1321 gtaggtcagc aggcggtcgc agctgcgcac aatatgccgc agatgaaacg caagcgaagg
     1381 tagctccagc ggacgcgcat caaaatccgc atccgtcagc ggaccacacc agcgatgcac
     1441 gtcttccttc gccagttcca aagcgtgcaa aaccgcgcgc gccacaggcg gaacatcggt
     1501 caacgtcccg cgcagccacg gttcaggttg ttgcgctgga gctacttttc ctgaatcact
     1561 catcaaagtc ccttcttctg ccggaattcg cgcaccacct ctgccggatc gcgatgttca
     1621 acatccacgg cttcgttcat ggcgcgcatc tcgtctgctg atattttatt tgccagatca
     1681 cccagtgcgc tgcgcagggc gggccatcgt ttcagcgcat catctcgcac cagcggaaca
     1741 gcctcatacg gcgggaaata atgcctgtcg tcttcgagca ccttcagacc aaactccgca
     1801 atctggccat cggtcgaatt agcagcgatc atgtccacct gatgcgcatg gagagcgcga
     1861 tacaacagtc cgagatccat ggtgcgcggc ggctccttga aatgcaggcc atagacttta
     1921 ctcaatccgg gaagcccatc cgggcgctgc tcaaattcat agcccacgcc caaacgccac
     1981 tgcggagtgt atcttgccgc atcgctcaac gtgttcaggt gcaaacgctg cgcatcctct
     2041 ccacgaacga ccatagcaaa tgtgttttca aatccgagcg gtggcataac ggtgatttga
     2101 taccgctgct gatacaaatt tcgcacagaa ttgagaacag tctgcggatc acgctccact
     2161 ggccgcttca ggatggcggt cagcgccgtg cccgtgtact ccacgtaagc gtcaatgcgc
     2221 cctgaaatca gtgcctgatg gcagatatag cttccggcga gataaaagcg gcgctccacc
     2281 ttcatcccgg ttttggcttc aatgtgctgg gcgagaagct ctcctaagat cacctgctcc
     2341 gtaaagttct ttgcgccgat gactggataa tcgggcctcg gaggaccaca acttaccaac
     2401 aaaaagcaca gcaatagtcc aggcaaaatc aaaacccgga tgccccatcc tttcgccgct
     2461 ggtttcgaca aaaggacgga ccttttcgca ggaaatcgcc tgttcacggc ttcccagctc
     2521 ccggcacccg cgcccatttc tccaccaatc caagcaggcc atccgcaccc agcgctagca
     2581 gcgctgcggg aatggccccg gcgagcacca gcctgttgtc caccgacgca actccacgaa
     2641 aaataaattc gcccagccca ccggccccaa cagccgccgc aatcgtagca ataccgacgc
     2701 aggtcacggt cgccgtgcgc aatccggcaa ggatcacgcc tgtggcgagt ggcaattcga
     2761 ctttcgtgag cagttgcatc tctgtcatgc ccaatgcgca cgccacatca cgaatggtcg
     2821 gatcaattcc tcggattccc gcataggtat tacgaatgat gggcaacaat gcgtatccgg
     2881 tgagcgcaac aatcgcaata cgcgcagccc gctcgcccag ccacggcagc ggcagaagaa
     2941 atccgaacat ggccaggctg ggaatggtct gaatcacatt cacgaagccg agaatcggcc
     3001 ttgcccagcg ctcatggcgc gtcagccaga ctccaacagg aatgccaatc agcgaagcaa
     3061 acagcatggc cgcgccggtc agccagagat gctccagcgt cagcgtgcgg atttccgcgc
     3121 catactgcgg aaggaaattg ctcaccgcgc tactccgcga tgaaatgcct gcgcgtattc
     3181 acgcacggct ggcacatcag aacctaaaaa gtcctcagca ggcagatcag ccacaacccg
     3241 cccctcttcg agcagcacaa tgcgcgaagc cagaaacagc gcctcatcca gatcgtgcgt
     3301 caccagaagc accgttttct tcagccgctg gagcaggtcc cgcaacatct cctgcatctc
     3361 tgcgcgtgtc agcgggtcga gcgcgccaaa tggctcgtcc atgagcagaa tcgccggatc
     3421 agccgcaagc gcccgggcca gaccaacccg ttgtcgttgt ccccctgaaa gctggtgcgg
     3481 ccatcgctgc gcaaatttct ccggctcaag cccaaccagc ttcagcaatt catgaattcg
     3541 gcccttgtcg gcgtggtttc cgttcagctc tggcacaagt ccggcatttc gctcgaccgt
     3601 gaaatgagga aagaggcccg tctcctgaat cacataccca atcccgcgcc ggagcaatac
     3661 tgcgtcctga tgggctatat catt
//
LOCUS       DASRBV010000151         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_567118, whole genome shotgun sequence.
ACCESSION   DASRBV010000151 DASRBV010000000
VERSION     DASRBV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567118"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFA02_05780"
     CDS             <1..241
                     /locus_tag="VFA02_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09395.1"
                     /translation="RKFVSFPKSVSCPEWGRKGGEFSVMASDLLTNYVRSSILKKQTS
                     ASHGKVAANPDQCPAAGAIHGCGRNLRGFQFHSGK"
     gene            249..2969
                     /locus_tag="VFA02_05785"
     CDS             249..2969
                     /locus_tag="VFA02_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09396.1"
                     /translation="MPTDGVVWTEGHRGLEAQRVLKDSAGQKAGVKVGDILVAANDKP
                     LPRYAALAREMFFTGPYGTIHYTIERGGVKLTNVQVILDVKDRSINQGFRLIALVYLG
                     IGFYVLFRRWTAPHAMHFYIFCLASFVLYAFKYTGKLNALDEIIYWGNIAANALQPAL
                     FLHFAQAFSEGRGKRRWLTGLIYLPGCCLTGLYVYAVQQWSATAVLEHRLDQIAIGYL
                     SFYYVLAAAVFFISYRREDQPLRRQQLKWLTRGTLVAIVPFTLLYAIPYMADWATPDL
                     LTKLAGISLLFLPLTFSWAIIRYRLMDTDLIFKRGVTYTLATAALVGLYFGLVAVAAE
                     IVHTRLPSAGVWGLIAAIIITAQLFDPLKRAIQDRVDRVFDRKRYDYRETLIEFGRGL
                     SSQTDLKALLRSIVERLPHTLLVARVAVFLAEKPGAYRLAEGHGIPQSALDGKEALDL
                     SFLDFDQPDAGSHIFLENPQQALHLTESERRTAALLDLNYYLPCRLQDRTIAVIGLGR
                     TTDGDFLSSEDMELLESLAGYIGIAIQNARLYQSLEQKISEYERLKEFNENIVESINV
                     GILAVDLEERIESWNAQMEVLCALPRREALGKRLAEIFPASFMDEFYRVKDEPGVHNL
                     YKFRLEMREGEPRTANIAIAPLVSRNFETVGRIILVDDITERTELEAQLSQSEKLSSI
                     GLLAAGVAHEVNTPLAVISSYTQMLAKQLRGDERLSPLLEKITSQTFRASEIVNNLLN
                     FSRTSSTEFRETDVNAIIRETLTLLEHQFKTAKVQTVVELEPELPPILGNAGKLQQVF
                     LNLFLNAKDAMADGGVLRISTEANGHVSVSIADSGSGIAPEHLQRIYDPFFTTKTAPK
                     QGQRRGTGLGLAVTYGIIQEHAGKIHVESQVGSGTTFFLEFPMLRKPAHV"
     gene            2962..4398
                     /locus_tag="VFA02_05790"
     CDS             2962..4398
                     /locus_tag="VFA02_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU09397.1"
                     /translation="MSEGTLAVTPPVASSHTEPAHRILIIDDEAAIRESLETLLTLEG
                     YRVDTAPDGEAGLERLGRATYDLVLLDLALPGKNGLEVLPLIREQHSELPVIMITAYG
                     TVDNVVEAIRAGAQNFIQKPWDNEKLLADIRSAIARYRVEEENIQLKRALKQRYNFEN
                     IVGKSDPMLRIFDLVAQVAPSRSTVLIQGESGTGKELIAKALHANSPRKDKPFVAVNT
                     GAMPSELLESTLFGHVKGAFTSAIAHKKGLFEIANGGTLFLDEIATMGLDTQAKILRV
                     LQDRRFMQLGGVQEIQVDVRIIAATNVDLRQAVREGKFREDLYYRLNVITVDLPPLRS
                     RREDIPLLATHFLKRFSEENNLPVRSLSPDAMRALIDYDWPGNVRELENVIERGVVLS
                     SGQTINSDLLPGHITGRNYSSSLLEHNPNASLFDIIEDVERRIIIDKLERCNWNQTDA
                     AEQFRIPLSTLNQKIKRLNIEIRKRVKE"
     gene            4643..5422
                     /locus_tag="VFA02_05795"
     CDS             4643..5422
                     /locus_tag="VFA02_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09398.1"
                     /translation="MPLTTLDRAAKKMVSAVQQYALLSGRALGDLFRRPFYGADIFTQ
                     ADVIGVGSIPIVILTGFFTGGVLALQTSASLSQFGAAGMTGQLVSFSMIKELGPVLTS
                     LMVSGRNASGMASELGSMMVTEQIDAMRALGTDPLKKLVMPRIVATIVMLFFLTIISD
                     VVGISGGGAVAVLMLGQDSSQYFHTAYQALRYADLIQGLVKPIFFGFIISTIGCYYGM
                     STRGGTQGVGRSTTQAVVAASVLIIATDFVISRFMIGIFGR"
     gene            5430..>6247
                     /locus_tag="VFA02_05800"
     CDS             5430..>6247
                     /locus_tag="VFA02_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09399.1"
                     /translation="MSQAAVAPELEQRPPDTPVVVFEHVSIGFEGKPVLQDISFSVRH
                     GETRILLGPAGVGKSVLLKLADGLLCPDEGRIFIFGEEISAMREKDLYPLRARIGIVF
                     QESALFDSLTVRDNVAYRLMEERVPQNEVEQRVTEALRFVELEHTLDKFPSELSGGMR
                     RRVAIARAIVSRPDLLLYDSPTGGLDPITSTTIIELIVKQRDVYHTTSLMVTHRLQDA
                     FLLASQRFNREANKMEPLPEGHSDPQTSFMVLNEGRLVFDGSTHDLVHSDDPFL"
BASE COUNT         1260 a         1567 c         1989 g         1431 t
ORIGIN      
        1 acggaaattc gtatctttcc cgaaatcagt aagttgtccg gaatggggaa ggaaaggcgg
       61 cgaattttct gttatggcaa gtgatttgct cacaaattat gtgcgttcgt ctattctgaa
      121 gaagcaaacc tcggcatcgc atggaaaggt cgctgcaaac ccggatcagt gtcctgctgc
      181 tggcgctatt cacggttgcg gccgtaacct tcgcggtttt caatttcatt caggaaaata
      241 attacacgtt gcccaccgat ggtgtggtgt ggacggaggg ccacagaggg ctggaagcgc
      301 agcgcgtcct gaaggacagc gccgggcaga aggccggggt gaaagttgga gacattctgg
      361 ttgcggcgaa cgacaagccg ctgccgcggt atgcagcgct ggcccgcgaa atgtttttta
      421 ccgggccgta tgggacgatc cattacacga tcgagcgcgg cggggtgaag ctgacgaatg
      481 tccaggtgat tctggacgtg aaggaccgca gcatcaatca ggggttccgg ctgattgcac
      541 tggtgtatct gggcatcggg ttctatgtgc tgttccggcg ctggacggcc ccgcacgcca
      601 tgcattttta tattttctgt ctggcgtcgt ttgtactgta cgcgttcaag tacacgggca
      661 aactgaatgc gctggacgag atcatttact ggggcaatat tgctgcgaat gctttgcagc
      721 cggcgttgtt cctgcatttt gcgcaggcat tttccgaagg gcggggcaag cggcggtggc
      781 tgaccgggtt gatttatctg cctggttgtt gcctcacagg gctttatgtt tatgcagtgc
      841 agcagtggtc ggcgacggcg gtcctggagc atcgcctcga ccagattgcg attggatatc
      901 tttccttcta ctacgttctt gccgcagcag tcttcttcat cagttaccgc cgggaagacc
      961 agcctttacg gcgccagcaa ctgaaatggc tcacgcgcgg cacgctggtt gcgattgttc
     1021 ctttcacgct tctgtatgcc attccttaca tggcggactg ggccacgcct gatctgctga
     1081 cgaagctggc cgggatatcg ctgttgtttc tgccgctgac cttcagttgg gcgattattc
     1141 gctaccggct gatggacaca gacctgatct tcaagcgcgg cgtgacctat actctggcca
     1201 cggcggcgct ggtcgggttg tattttgggc tggtggcggt ggcggcagag attgtccata
     1261 cgcggctgcc gagcgcaggc gtgtgggggc tgattgcggc catcattatt acagcgcagt
     1321 tgtttgatcc gctgaagcgc gcgattcagg accgcgtaga ccgcgttttt gaccgcaagc
     1381 gctacgacta ccgcgagacg ctgattgaat ttggacgcgg actgagttcg cagaccgacc
     1441 tgaaggcgct gctgcgctcg attgtggagc ggctaccgca tacgctgctg gtagcgcgcg
     1501 tggcggtttt tctggcggaa aagccagggg cgtaccggct ggccgagggc catgggattc
     1561 cgcaatctgc gctggatggg aaagaggcgc tggacctgag ttttctggat ttcgaccaac
     1621 cggatgcagg gtcgcacatc tttctggaga acccgcaaca ggcgctgcat ctgacggaaa
     1681 gcgagcggcg cacggcggcg ctgctcgatc tgaactatta tctgccgtgc cggctgcagg
     1741 accgcacgat tgcggtgatt ggcctggggc ggacgacgga cggggatttt ctttccagcg
     1801 aagacatgga actgctggaa tcgctggccg ggtatatcgg tatcgcgatc cagaatgcgc
     1861 ggctgtatca gagcctggaa cagaagattt cagaatacga gcggctgaag gaatttaacg
     1921 agaacattgt ggagtccatt aatgtaggca ttctggcggt ggacctggaa gagcgcatcg
     1981 agagctggaa tgcgcagatg gaagtactgt gcgcgctgcc gcggcgcgag gcgttgggga
     2041 agcggctggc ggagatattt ccggcatcgt ttatggacga gttttaccgt gtgaaggacg
     2101 agccgggggt gcataacctt tataaattcc ggctggagat gcgtgaaggc gagccgcgga
     2161 cggcgaacat tgcgattgca ccgctggttt cgcggaattt cgagacggta gggcgcatca
     2221 tcctggtaga tgacattacc gaacgcactg aacttgaggc gcaactgagc cagtcagaga
     2281 agctgtcgtc tattggcctg ctggcagcgg gagtggcgca cgaagtcaat actccgctgg
     2341 cggtgatttc gtcatatacg cagatgctgg cgaaacagtt gcggggcgat gaaaggcttt
     2401 cgccactgct tgagaagatc acgtcacaga ctttccgtgc gtcggagatt gtgaataatc
     2461 tgctgaattt ttcgcgcacc agcagcacgg aattccggga gacggacgtg aatgcgatta
     2521 tccgtgagac gctgacgctg ctggagcacc agttcaagac agcgaaggtg cagaccgtag
     2581 tagagctgga gccggagctg ccgccgattc tgggcaatgc gggcaagctg cagcaggtgt
     2641 tcctgaacct gtttctgaat gcgaaggacg cgatggcgga cgggggcgtg ctgcgcattt
     2701 cgacagaggc caatggtcat gtttcggttt cgattgcgga cagcggctcg ggaatcgcgc
     2761 cggagcattt acagcgtatc tatgacccat ttttcacgac aaagaccgct cccaaacaag
     2821 gacagcggcg cggcacgggg ctaggcctgg cagtgacgta cggcattatt caggagcacg
     2881 ccggaaaaat ccatgtagag agtcaggttg ggtccggcac tacgttcttt cttgaattcc
     2941 cgatgttgag gaaacctgcg catgtctgaa ggcacccttg cagttacgcc acctgtcgct
     3001 tcatcccata cagaacctgc gcaccggatt ttgattattg atgatgaagc ggcgatccgt
     3061 gagtcgctgg agacgctgct gacgcttgaa ggctatcgcg tggacacggc gcctgacggc
     3121 gaggcaggac tggaacgtct ggggcgcgca acttacgatc ttgtgctgct ggaccttgcg
     3181 cttccgggca agaacgggct ggaggtgctg cctctcatcc gcgagcagca cagcgagctt
     3241 cctgtgatca tgattacggc ctacggcacg gtagacaatg tggtcgaggc gatccgcgcc
     3301 ggggcgcaga actttatcca gaagccgtgg gacaacgaga agctgctggc cgatatccgc
     3361 tcggcgattg cgcggtatcg cgtagaagaa gaaaacatcc agctcaagcg ggcgctgaag
     3421 cagcggtata actttgaaaa cattgtgggc aaaagcgacc cgatgctgcg catcttcgat
     3481 ctggtggcgc aggtggcccc aagccgctcg acggtgctga ttcagggcga gagcggaacg
     3541 ggaaaagagc tgatagccaa ggcgctgcat gccaattcgc cgcgaaagga caagccgttt
     3601 gtcgctgtca atacgggggc gatgccctcg gagctgctgg aatccacgct tttcgggcat
     3661 gtgaagggcg catttacttc ggcgattgct cacaagaagg ggctgtttga gattgccaac
     3721 ggtggcacgc tcttcctgga cgaaattgcg accatgggcc tggacacgca ggcgaagatt
     3781 ctgcgcgtcc tgcaggaccg ccggtttatg cagcttggcg gggtgcagga gatacaggtg
     3841 gatgtgcgca ttatcgcggc cacgaacgtg gatttgcgcc aggcagtgcg ggaagggaag
     3901 ttccgcgaag acctttatta ccggctgaac gtcatcacgg ttgatcttcc gccgctgcgc
     3961 agccggcgcg aggacattcc gctgctggcg acgcattttc tgaagcggtt ttcggaggag
     4021 aataatctgc cggtgcgttc gctttcgcca gatgcgatgc gggcgctgat tgattacgac
     4081 tggccgggca atgttcgtga gctggagaat gtgatcgagc gcggcgtggt gctttcttct
     4141 gggcaaacga tcaactcaga cctgctgccc gggcatatta cggggcgcaa ttattcgtcg
     4201 agcctgcttg agcacaatcc caatgcttcg ctgttcgaca ttattgaaga cgtggagcga
     4261 cgcatcatta ttgacaagct tgaacgctgc aactggaacc aaaccgatgc tgctgaacag
     4321 tttcgcattc cactttccac gctgaaccag aagatcaagc ggctgaacat tgagatacgg
     4381 aagcgggtga aagagtagag cgcttcttca gttgttagtc cccagttgct ggttcgtgat
     4441 tgttgcagtt ttcgtttcac tgtgatccac gaaagggatg gggtcggcat tccgggtggg
     4501 gtgaagatct taagactgga gctgaggact ggcaaccggg gctaactaac aactataccc
     4561 gcgtctggcc ccgggacggc ggagtccgtg gtaccctcac tttgtaagca gcatctaagc
     4621 gataagcaaa atgaacggga ccatgccact taccacgctg gatcgagctg caaaaaagat
     4681 ggtcagcgca gtgcagcagt atgcgttgct ttcggggcgc gcgctgggcg atctgtttcg
     4741 ccggccattt tatggagcgg acatcttcac gcaggctgac gtgattggtg ttggctcgat
     4801 tccgattgtc atactcaccg gatttttcac cggaggcgtg ctcgcgctgc aaacatctgc
     4861 gagtctctcg caatttggcg cggccggaat gacggggcag cttgtcagct tttccatgat
     4921 caaggagctg gggccggtac tgaccagcct gatggtctcc ggacggaacg catccgggat
     4981 ggcgagcgag cttggctcga tgatggtgac cgagcagatt gacgccatgc gggcgctggg
     5041 caccgatccg ctgaagaagc tggtgatgcc gcgcattgtg gcgacgattg tgatgctgtt
     5101 ctttctgacc attatttccg acgttgttgg catctcaggc ggtggagcag tggccgtgct
     5161 gatgctgggg caggacagtt cgcagtattt ccacactgct tatcaggcgc tgcgctatgc
     5221 agacctgatc caggggctgg tgaagcctat tttcttcgga tttattattt ccaccattgg
     5281 ttgttattac ggaatgtcca cacgcggcgg gacgcaggga gtgggccggt ctacaacgca
     5341 ggccgtggtt gccgcgtccg tgctgattat tgctacggat ttcgtcatca gccgctttat
     5401 gatcggcatc tttggcagat gaggggcgca tgagccaggc cgcagtcgct ccggagctgg
     5461 aacagcgccc gccggacacc ccggttgtcg tcttcgagca tgtctcgatt ggctttgagg
     5521 gtaagccggt gctacaggac atctcttttt ctgtccgcca tggcgaaacc cgcattctgc
     5581 ttggccctgc gggggtaggc aagagcgtgt tgctgaagct ggccgacggt cttctttgtc
     5641 cggacgaagg acgtattttt atttttggcg aagagatcag cgcgatgcgg gagaaggacc
     5701 tgtatccctt gcgtgcgcgg atagggattg tttttcagga gagcgcgctg tttgattcgc
     5761 ttaccgtgcg tgacaatgtg gcctaccgtc ttatggaaga gcgtgttcct caaaatgaag
     5821 ttgagcagcg cgtgactgag gcgcttcgct tcgtggaact ggaacacacg ctcgataaat
     5881 ttccttccga gctttccgga ggcatgcgcc gccgcgtggc gattgcgcgg gccattgtga
     5941 gccgtccgga tttattgctt tatgattcgc cgacgggcgg gcttgatccg attacatcta
     6001 cgacgatcat cgagctgatt gtgaagcagc gcgatgttta ccatacgacg tcgctgatgg
     6061 tgacgcaccg tttgcaagat gcctttctgc tggcgtcaca acgtttcaat cgcgaagcga
     6121 acaaaatgga gccgctgccg gaagggcatt ctgatccgca gaccagcttt atggtcctga
     6181 acgaagggcg gcttgtgttt gatggatcca cgcatgatct ggtgcattcc gatgatccgt
     6241 tcctgaa
//
LOCUS       DASRBV010000152        13393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_672493, whole genome shotgun sequence.
ACCESSION   DASRBV010000152 DASRBV010000000
VERSION     DASRBV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672493"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFA02_05805"
     CDS             <1..1197
                     /locus_tag="VFA02_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CadC family transcriptional regulator"
                     /protein_id="HZU09400.1"
                     /translation="RAFWLRWSPDGKLLRFTVLDPQTGDPSLWEYSASSRKLRRLLDG
                     WSRPSAECCGSWTPDGKVYVFGSTHDGPADIWQMRGGNLDRNPEKLTNGPLEYRSPAT
                     GTESNRVFFTGVDYRREMLKYSAAAHRFLPLENNLGSTALVEYSRDGQWVAWLNASDN
                     TLWRSRADGTERLQLTTPSTPALSIFSMRWSPDNRSLALMARKAGGLWAIYLIDANGG
                     NLQPVLEENRGQADPNWSPDGGSILFGRVPDIMGGESQPKAIFSVDLQNHAVEKVPGS
                     DGLFSPRLSPDGRYIAALRSDQKTMMLFDRQTQRWTALVEDGAADPVWASNSRAIYYQ
                     NFLEPGEPVYRIDLPSGATHKIATLTDLQPAYASDYRLIALAPGDQPIVNTQSSSVNI
                     YSLGLK"
     gene            1290..3485
                     /locus_tag="VFA02_05810"
     CDS             1290..3485
                     /locus_tag="VFA02_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZU09401.1"
                     /translation="MRKWLKVFVCGVVLVLTQTSILLAQNNKRPRIGLVLDGGGALGL
                     AHIGALLWMEEHHIPIDMVAGTSMGGLVGGAYAAGETPEQIQQLVEGIDWNEILSDVP
                     PYRDLSFRRKEDRSQYPVSLLLGVGRKVRSQEGYKAGQQVQLLLDKIALPYSQVENFD
                     DLPTPFACVATDLVSGKEHVFRSGSLALAMRATMSLPGIFAPVRTDGALYADGGLLNN
                     LPVDVAKQMGADITIAIYLQTASITPTERLSTVGVLGRSLSVMIAANELKSMEQADVL
                     VTVPLADYTALDYDKTTELIRKGYEAAQSKAAVLNRFSVDDATWKEYLAERAKRMRTV
                     PVPQFVAVSGVSKQDAQQIRQELAPDLRKPIQPDKVARQLTAVASDERVSNLSYGLTT
                     NLAGTPGIAVTGRPDPYGRNILRPLLLIDGWDYKNVTLSVGARLTMFDLGRYGAELRN
                     DVLLGSEYLASSEYMWPLTASRRLFVAPRLFGDNAPLNIYSRKGLLAEYRNRTMGGQI
                     DAGYFFGRSAELRVGYEAADQRLYPNVGDPQLYPRVTGREGDLHVRFTLDRLDNPLTP
                     MRGLRLKARSEWWDAKPEAGTSFPLLEVSSINFAPLTDRSSLYLGISGGSTLWEKPGG
                     LPPFTLGGSFRIPAYNTNELLTDQYFLFQGGYVRQTGALSPLEGGKVLFFAGADVGKG
                     YYLEKTSHLPSDGSAGFIINTLFGPVVFGGAIGDVGHYKFFIELGKTYF"
     gene            complement(3595..4833)
                     /locus_tag="VFA02_05815"
     CDS             complement(3595..4833)
                     /locus_tag="VFA02_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZU09402.1"
                     /translation="MGRFTGVLGLLTMLCLAWIFSTNRKAIRWRTVFWGIGLQLLFAF
                     IVLDFSWGQRALAMAGAAVTKLLSYAYAGSGFVFGELGKQHSSFGSIFAFQVLPTIIF
                     ISAFFAVLYHVGIMQLLIRGFAWLMQWTLRVSGAESLNVAASIFMGQTEAPLTVRPFL
                     PDATRSELMTIMTSGMAHVSGGIMAAYILYGIEAQHLLAAVIMTAPGTILVSKMLVPE
                     TEIPATEGTVHMPKDSEHKEENLLGAIARGTIDGGRLAFNVAIMLISFIALIALTNGI
                     LGGIHNWLGMYHVPFPSRLDSILGAIFAPVAWLIGVPWHDAKIVGNLLGTRMVINEVV
                     AYSLLGAQKAMLDFRSFTIATFALCGFANLSSIGIQIGGIGALAPNRRNDLARLGLRA
                     MLAGTMANLLSASIVGILMR"
     gene            complement(4858..6213)
                     /locus_tag="VFA02_05820"
     CDS             complement(4858..6213)
                     /locus_tag="VFA02_05820"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZU09403.1"
                     /translation="MHAIDLIRKKRDGEELSAEEIRFLAEGAARSFVPEEQLAAWLMA
                     AFLRGLSLAELDALTNAMRFSGETFDPSPLKKFAVDKHSTGGVGDKTSLLVAPIAAAA
                     GLAVPMISGRALGHTGGTLDKLESIPGYRTQLSLREMERVLTEAGASIVGQTKALVPA
                     DRVLYSLRDRTSTVESPYLICASIMSKKLAAGLNGLVLDVKTGSGAFLRNKQDALFLA
                     ALMVETGERAGTRTAALLTNMDQPLGSFAGNWVEIWESFDLLQGKRNPLNEDLRELSL
                     VLAGWMLYLGGITSTAENGHERAEEILASGAALDVFIKMVAAHGGDISVFTYPEKFHQ
                     PAASKDFLSDRSGYLAQIDCTAVGWAVQRLGAGRERAGGPIDPHAGIRMHVKSGSKVE
                     AGHKLCTMFSSDARRFAEPEALLRKALVIADKPGPRQPLVQEIITTENRKEFLKTTNR
                     P"
     gene            complement(6200..6628)
                     /locus_tag="VFA02_05825"
     CDS             complement(6200..6628)
                     /locus_tag="VFA02_05825"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZU09404.1"
                     /translation="MPQLTPEKLEQLREAATAAAKHAYAPYSGFRVGAALLLSDGSIV
                     TGCNVENISYGLTICAERSAVVRAIAESGLKRKIAAVAVTNLEGAASPPCGACRQVLG
                     EFVTQDALVYFADGKGGMTAVPFAELLPHSFSDWKSDARH"
     gene            6876..9044
                     /locus_tag="VFA02_05830"
     CDS             6876..9044
                     /locus_tag="VFA02_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZU09405.1"
                     /translation="MDDLTREFLIESQEGLDRMERCLTELEELPEDRELLADIFRSVH
                     TIKGTTGFLGFLRLEKLAHAGENLLGLLRDGKMVANAEIISALLSLLDALRGILSNIE
                     ASGSEGEGDDAPMIARLQELQESAAEAKADAPEKPKKKRRLKAKKQPKESAMTIQVAS
                     PVPESKAIEVYQAEPQVPQPSRAMANTAESTLRVDIDLLNRMMNLVGELVLTRNQILQ
                     RVGAESDLTLLSRRLDMVTADLREAVMKARMQPVSHVFSKFPRMVRDLAQQLNKRVRL
                     VMEGQETELDKSLLEAIKDPLTHSVRNAIDHGIEAPHLREAAGKDAEGTVKLRAYQEG
                     SYVIIEVSDDGGGMKVERIRDKALERKLITRERAAQLSDRELMQLIFLPGFSTAEAIT
                     NVSGRGVGMDVVRTNVEKIGGKVEIDSRPGKGTTLRLRIPLTLAIVPALIVFSRGQSF
                     ALPQGALSELVHLSAEQAKTQVEWMEGAALYRLRGKLLPLVFLDSLLKQREEQAREGE
                     AYIAVLNAEGRRYGLIVDGLADPEEIVVKPLSSVLKQIGLFSGATVLGNGEMALILDP
                     GAIATQANIGMALEEDAHEVIEAGTRNGATEYLLMQSGQEHAAVPLDKVLRIERIPRA
                     RMEWLGGVPVLRFEGSLLPLRDTGSAGEEMTVVVCRDGSRHVGVAVSQVLDVADGRPL
                     EEAGTHAAARNVTLLHEKVTSIVPLEVIPALPVQTLEVTQ"
     gene            9041..9508
                     /locus_tag="VFA02_05835"
     CDS             9041..9508
                     /locus_tag="VFA02_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZU09406.1"
                     /translation="MSAIMLAEDVIEMCSVRVGETLFGVPVMRVLEILGKPATHPVPL
                     APEFIGGLAHYRGEVLTTVSLRRLLKMPPCGSSEDVLVFEGEDGYFGLLVDEVSEVLT
                     VSPADFEGNPSTLDDRRKALFAGTYKLKDGLLVMLDPERLDPVRLTEMDLRSA"
     gene            9505..9873
                     /locus_tag="VFA02_05840"
     CDS             9505..9873
                     /locus_tag="VFA02_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU09407.1"
                     /translation="MKALIVDDSSFIRSFLRALLESHALECVEAENGQVALDILAGST
                     FGLALVDVNMPVMNGLEFVKRLRGNPEHKDMKVVMVTTEADHAFIEAALEFGADEYLM
                     KPFDEESLLEKLQMVGLRQA"
     gene            9878..10963
                     /gene="cheB"
                     /locus_tag="VFA02_05845"
     CDS             9878..10963
                     /gene="cheB"
                     /locus_tag="VFA02_05845"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZU09408.1"
                     /translation="MQARPVRVLVVDDSLVMRSLLRMVMASDPTIELAGMAADGVSAL
                     DAVERLKPDLVLLDIEMPRMNGIEMLAELRARKHPVKVIMCSTLTRRGAAITLEALAR
                     GATDYVTKPTAQHGAADAVATLTRELLPKIRGLFPSGRNETDRAVTREAQAEGQRKPA
                     SIVVIGVSTGGPTALEALLPQLPQEFPAPIVIAQHMPRLFTSLLAERLDRVCDLRVRE
                     AVAGERIAAGCIYLAQGDQHLQLTRAAFGQGHVFRLFPPAAGDFCRPSVDMLFRSAAD
                     VLGDGVVGVVLTGMGSDGLEGCRAIRKAGGQVIVQDRATSAVWGMPGAVMEAGLAHRV
                     LPLPSIAAELARVCAVPSISVLTRATA"
     gene            10963..11778
                     /locus_tag="VFA02_05850"
     CDS             10963..11778
                     /locus_tag="VFA02_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZU09409.1"
                     /translation="MPCSEIDLTFLRHFLQERSGNVLGPERDHLFEARLYPIWQSLGM
                     SGLDELVEKLRFATDPAFEQAVVEAMTINETSFFRDHAPFDLLREELLPKLIRARAEE
                     RRLRFWSAACSSGQEAYSLAMLLRESFPQVADWNIEILGTDIHAAMVRRAHAGRYQRM
                     EINRGLPARYLLKYFRRDGEEWEAVPELRAMCRFEQRNLAYFSPFLTQFDGILLRNVL
                     FYFSEARQMSILRSVRSCLYPDGFLLLGPSERADISGQWRPFFHGNVCYYRPC"
     gene            12770..>13393
                     /locus_tag="VFA02_05855"
     CDS             12770..>13393
                     /locus_tag="VFA02_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZU09410.1"
                     /translation="MARQTATDVLDLFHPVTAEWFQTALGEPTAPQRMGWPAIARGES
                     TLILAPTGTGKTLTAFLWCLDRLVFGERARGCRVVYVSPLKALAVDVERNLRMPLAGI
                     AERAQRMGVAFHGPEISVRTGDTPQNERSRFRRRPGEILITTPESLYLLLTSESAAAL
                     RTVETVIVDEIHALVPTKRGAHLALSLERLEAMVERPLQRIGLSATQR"
BASE COUNT         2865 a         3439 c         4067 g         3022 t
ORIGIN      
        1 cgtgcctttt ggctgcgatg gtcgccggac ggaaaattgc ttcggttcac cgtgcttgat
       61 ccgcagactg gagacccttc gctctgggaa tatagcgcat caagccgcaa gctgcgccgc
      121 ctgctcgatg gatggagccg gccttctgcc gaatgttgcg gaagctggac gccggacgga
      181 aaggtatacg tcttcggttc cacacatgac gggccagcag atatctggca gatgcgtggg
      241 ggaaatctcg accgaaatcc tgaaaagctg acgaatggac cgctcgaata ccgttctcct
      301 gcgaccggga ccgaatccaa ccgtgtcttc tttactggcg tggattaccg gcgcgagatg
      361 ctcaagtact ctgctgctgc gcaccgcttt cttcctttgg aaaataacct cggttcgacg
      421 gcgctggtcg agtattcgcg cgacggtcag tgggtagcgt ggctcaatgc atcggacaat
      481 accctctggc gcagccgcgc cgacggaaca gagcggcttc aactgaccac accttcaacc
      541 ccggcgcttt cgattttcag catgagatgg tcgccagaca atcgctcgct ggcactgatg
      601 gcacgcaaag caggagggct ttgggccatt tatctgatag acgctaacgg cgggaacctt
      661 cagccggtac tcgaagaaaa tcgcggacag gccgacccta actggtctcc ggatggaggc
      721 tcgattctct ttggccgcgt gccagatatt atgggcggtg agtcgcagcc aaaagctatc
      781 ttcagtgttg accttcagaa ccatgccgta gaaaaggtcc ctggttcaga tggattgttc
      841 agtccgcgcc tctcgccaga cggccggtac atcgccgctc tgcgttcaga ccagaagacc
      901 atgatgctct ttgaccgaca gacgcagcgc tggacggcac tggtggaaga tggtgcagca
      961 gatccggtat gggccagcaa cagccgtgcc atctattacc agaattttct ggagcctggt
     1021 gagccggttt atcgtattga tctgccatca ggcgcaacac ataagattgc aactctgacc
     1081 gacctgcaac cagcctacgc ttccgattac cgcctcatcg cgctggcccc cggagaccag
     1141 cccattgtga acacccagag ttcttcggtc aatatctatt ctctgggcct gaaatagcgg
     1201 gtattcatcc ggatacatat tctggggatt tctgcttctt tttctggaaa atgtgcctgt
     1261 ttctttggtc agatagactc tgggacatca tgcgcaagtg gctgaaagtc ttcgtgtgcg
     1321 gggttgttct tgttctgacg caaaccagca ttctattggc gcagaacaac aagagacccc
     1381 gtattggcct ggtgctcgat ggcggaggcg ctttagggct cgctcatatc ggtgcgctcc
     1441 tctggatgga agaacaccac atccccatag atatggttgc cggtactagt atgggcgggc
     1501 tggttggagg cgcatacgct gcgggcgaga ctccagagca gattcaacaa ctggtcgaag
     1561 gcattgactg gaatgagata ctcagtgatg tcccgcctta tcgagacctc tcctttcgcc
     1621 gcaaggagga ccgcagccaa tatcctgtat ctctacttct aggcgttggg aggaaggtcc
     1681 gctcgcagga aggttataaa gcaggacaac aagtgcaatt gctactcgac aagattgctt
     1741 tgccttattc acaggtcgag aacttcgatg atttgcctac tccatttgcg tgcgtggcga
     1801 cagacctggt gtccggtaag gagcatgtct tccgttccgg ctctctggcg ctggcaatgc
     1861 gcgcaaccat gtcgctgcct ggaatctttg cgcccgtacg gaccgacggt gctctttatg
     1921 cagatggcgg gctgctgaac aacctgccgg tagatgtcgc aaaacagatg ggcgcagaca
     1981 tcacaattgc catctatttg caaactgcat ctatcacgcc cactgagcgg ctttccacag
     2041 tcggtgttct tggacgcagc ctgagcgtga tgattgcggc gaatgaactc aaaagcatgg
     2101 agcaggccga cgttctggta actgttcctt tggcagacta tacggcgctc gattatgaca
     2161 aaacaaccga acttatcagg aaaggttatg aggcagcgca gagcaaggcc gctgtgctca
     2221 acagattttc agtagatgac gcgacgtgga aagagtatct ggcggaacgc gcaaagagga
     2281 tgcggacagt accggtgccc cagtttgtgg cagtaagcgg agtgtcgaaa caggacgcgc
     2341 agcaaatcag gcaggagctt gcgccggacc tccgcaagcc gatccagcct gacaaagttg
     2401 cacgccagct tacggctgta gcgagtgacg agcgcgtttc caacctgagc tatgggctaa
     2461 cgacaaatct tgctggcact ccgggaatag ccgttaccgg caggccagat ccgtatggac
     2521 gcaatattct gcggccactg ctgctgattg atggctggga ctataagaat gtgacgctga
     2581 gtgtgggcgc ccggcttacg atgtttgatc tggggaggta tggcgcggag ttgcgcaatg
     2641 acgtattgct gggttcagaa tacctggcgt cttcagaata tatgtggccg ctgacggcat
     2701 cgcggcgtct gtttgttgct cccagattgt ttggcgacaa tgctccgctc aatatctatt
     2761 caagaaaagg gctgcttgcg gaatatcgta accgcacaat ggggggacag atagacgccg
     2821 gctatttctt tgggcgctcg gctgagttac gggtgggcta cgaagctgcc gatcaaaggc
     2881 tctaccccaa tgttggtgat ccgcagcttt atccgcgcgt aaccggccgc gaaggcgacc
     2941 tgcatgtgcg ctttaccttg gatcggctgg ataatccgct gacaccgatg cgcgggcttc
     3001 gcctgaaggc ccgttctgaa tggtgggacg cgaaaccgga agcgggaacg tcatttcctc
     3061 tgctggaagt ttccagtatt aactttgcac cgcttactga caggtcttct ttgtatctcg
     3121 gcatttccgg tgggagcact ctttgggaaa aacctggagg cttacctccg tttaccctgg
     3181 gagggtcgtt tcggattcct gcttacaaca cgaatgagct tttaacggac cagtattttc
     3241 tctttcaggg tggatacgta cggcagaccg gagcactttc tccactggaa ggaggcaagg
     3301 tattgttttt tgccggagcg gacgttggaa aaggctatta tctggaaaaa acttctcatc
     3361 tgccttcgga cggttcggca ggattcatca tcaacacgct ctttggtccg gttgtctttg
     3421 gaggagcaat tggagatgta gggcactata aatttttcat cgaattagga aaaacctatt
     3481 tttaggaagc atctctcagg acctggttgg gaaactcatc cagcctcgaa gaaaaaaata
     3541 cagcaacgtt ccgattacgc tcaaaatagc aactcacatc ggatattgca tacattagcg
     3601 catcaggatc ccgacaattg aagctgacag caggttcgcc atcgttccgg cgagcatggc
     3661 tcgaaggccg aggcgtgcga ggtcgttacg gcggtttggt gcgagcgctc cgatgccgcc
     3721 aatctgaata ccgatggagc tgagattggc gaatccgcaa agtgcaaagg tggcaatggt
     3781 aaaggaacga aagtcgagca tggccttctg cgctcccaga agcgaatacg ccacaacttc
     3841 attgatgacc atgcgcgtgc ccaacaaatt gccaacgatc ttcgcatcat gccacgggac
     3901 gccgatcagc catgcgacag gagcaaagat ggcgccgagg atggaatcga gcctggatgg
     3961 aaatggaaca tggtacattc ccagccagtt gtgtatcccg ccaagaatgc cgttggtcag
     4021 cgcaatcagc gcaataaagg agatgagcat aatggcaacg ttaaaggcaa gccgcccgcc
     4081 atcaatcgtg cctcgtgcaa tggctccgag cagattttct tctttgtgct cggaatcttt
     4141 gggcatgtgg actgtgcctt ccgtcgcggg tatttctgtt tccggcacca gcattttgga
     4201 gacgagaatc gtacccggcg cagtcataat gacagctgcc agcaagtgct gcgcttcgat
     4261 gccataaaga atgtaggccg ccataatgcc tccggaaaca tgcgccatac cgctggtcat
     4321 gatggtcatc aactcggagc gtgttgcatc aggcagaaaa gggcggaccg tgagcggcgc
     4381 ttctgtctgg cccatgaaaa tgctagccgc gacattcagg ctttcggcgc cgctgacgcg
     4441 caatgtccat tgcatgagcc acgcaaaacc gcggatgagc aactgcatga ttcccacatg
     4501 gtagagcaca gcaaaaaacg ccgaaataaa gataatagtc ggcagaacct gaaaggcgaa
     4561 gatgcttcca aaagaagagt gctgtttacc tagttcccca aagacaaagc cggacccggc
     4621 ataggcgtag gaaagcagct tcgtaacagc agcgccagcc atagccaggg cgcgctggcc
     4681 ccaggaaaaa tcgaggacga taaaggcaaa gagcagttgc agcccaatgc cccagaaaac
     4741 cgtgcgccag cgaatggcct tgcggttggt ggaaaagatc catgccaggc agagcatggt
     4801 gagcagtcct aaaacgccgg tgaatcgtcc caaattgttc tcctgttcac aactatccta
     4861 tgggcggttt gtggttttga ggaactcttt tctgttttct gtcgtgatga tttcctgaac
     4921 gagtggttgc cgcggtcccg gtttgtcggc gatgacgagt gccttgcgta aaagtgcttc
     4981 tggttcagcg aagcgcctgg cgtcgcttga aaacatcgtg cagagcttat gaccagcttc
     5041 gactttcgag ccagatttta cgtgcattct gattcccgca tgaggatcaa tgggcccacc
     5101 tgccctttcg cggcctgctc cgaggcgttg cacggcccag ccaacggccg tgcagtctat
     5161 ttgtgcaaga tagccagagc gatcggaaag aaagtcttta ctcgctgcag gttgatggaa
     5221 tttttccgga taagtgaaaa cagatatgtc tccgccgtgg gctgcgacca tttttatgaa
     5281 gacatcgagc gctgcaccgg aagcgaggat ttcttcggca cgttcgtgtc cgttttcggc
     5341 cgtgcttgtg atgccgccca ggtagagcat ccagccagcg aggacaagcg aaagctcgcg
     5401 caggtcttca ttcagcgggt tgcgcttgcc ctgaagaagg tcgaacgatt cccatatctc
     5461 gacccagttt ccagcaaagc taccgagcgg ctggtccatg ttggtcagca gcgcagcggt
     5521 gcgtgttcca gcgcgttctc cggtctcaac catcaaggca gccaggaaaa gagcatcttg
     5581 tttgttgcga agaaatgcac cggaaccggt ttttacatcc agcacaaggc cgttgagtcc
     5641 ggcggcgagc ttcttgctca taatagaggc acagatgagg taggggcttt ctaccgtgga
     5701 tgtgcggtcg cgcagcgaat agagaacgcg atcggcgggg accagggcct tcgtctggcc
     5761 gacgatgctt gcccctgctt cggtgagcac gcgctccatc tctctgagcg aaagctgcgt
     5821 gcggtagccg gggatgcttt cgagcttgtc gagagtgcct ccggtgtgac cgagggcgcg
     5881 gccgctgatc atagggacgg cgagtccggc agcagcggcg atgggcgcaa ccaaaagtga
     5941 ggttttatcg cctacgccgc cagtggaatg tttgtccacg gcgaattttt taagcggcga
     6001 agggtcgaag gtctcgccgg agaagcgcat cgcgttagta agcgcgtcca gttcggcaag
     6061 agaaagtcct cgaagaaaag cggccatcag ccaggcggca agctgttctt cgggaacaaa
     6121 gctgcgcgcc gctccctcag caaggaagcg gatttcttct gcggagagct cttctccatc
     6181 gcgctttttg cgaatcagat caatggcgtg catcggactt ccagtcagaa aagctgtgcg
     6241 gcagcagttc ggcaaaagga acggcagtca taccaccctt accgtctgcg aaataaacca
     6301 gggcatcctg tgtgacgaac tcgcccagca cttgccggca tgctccgcag ggtgggctgg
     6361 cagcaccttc aagatttgta actgcaacgg cggcgatttt gcgtttcaga ccggattctg
     6421 caatggcgcg gacaacagca gaacgttcgg cgcaaatcgt caggccatag gagatgttct
     6481 cgacgttgca tccagtgacg atggagccat cgctcagaag cagtgccgcg cctactcgaa
     6541 agccggaata cggcgcatag gcatgttttg cagcggcggt ggcggcttca cgcaattgct
     6601 ccagcttttc cggtgtaagt tgaggcatcg ctttttgagg ctaccgggag agagggctgg
     6661 cgtgcaactt cagcggttcg caagatacca ccacctgtac gaggcaattc cgcgggccgc
     6721 agtgacgaaa gttacgtcaa cccgaagttt gtacgcggtg tgtcctgttt ttgctctgtt
     6781 ccacaatgtt ccccgtggaa cattcctcct ccagcacgtg cagccaatga gagcacgttg
     6841 ttcaagtttt ttcttctttt cccgataacc cactcgtgga cgatctgaca cgcgaatttc
     6901 tgattgaaag ccaggaaggt ctggatcgca tggagcggtg cctgacggag cttgaggagc
     6961 ttccggaaga ccgcgagctt ctggccgata tcttccggtc ggtccatacc atcaagggga
     7021 cgaccggatt tctgggcttc ctgcgtctgg aaaagcttgc ccatgctggg gaaaacctac
     7081 tgggattgtt gcgcgacggc aagatggtag ccaatgcgga gatcattagc gcgctgttgt
     7141 cgctgctcga tgcgctcaga ggcattctga gcaatattga agcatccgga agcgaaggcg
     7201 aaggtgatga cgcgccgatg atcgcgcgtc tgcaggaatt gcaggagagc gcggcagagg
     7261 cgaaagcgga cgccccggaa aagccaaaga agaagcgcag actgaaggcg aaaaagcagc
     7321 ccaaagagag tgcgatgacg attcaagtcg catcgcctgt cccggaatca aaggccattg
     7381 aggtctatca ggctgagcct caggtgccgc agccgtcgcg cgcgatggcc aacacggccg
     7441 aatccacctt gcgcgtagat atagacctgc tcaatcgcat gatgaatctg gtaggcgagt
     7501 tggtgttgac gcgcaatcag attctccagc gcgtaggtgc agagtccgac ctgacgttgc
     7561 tttcacggcg tcttgacatg gttacggcgg acctgcgtga agcggtaatg aaggcacgca
     7621 tgcagccggt aagccatgtg ttctccaagt ttccgcgcat ggtgcgtgac ctggcgcagc
     7681 agttgaacaa gcgcgtacgg ctggtgatgg aagggcagga gacagagctt gacaaaagcc
     7741 tgcttgaggc catcaaggac ccgctgaccc attcggtgcg caatgccatt gaccatggca
     7801 tcgaggcccc gcatttgcgc gaggccgcag gcaaagatgc tgagggcacg gtaaagctgc
     7861 gggcctatca ggaaggcagc tacgtcatca ttgaggtctc cgatgatggc gggggaatga
     7921 aggtcgagcg cattcgtgac aaggcgctgg agcgcaagct catcacgcgt gagcgagcgg
     7981 cgcagctctc tgaccgcgaa ctgatgcaat tgatctttct gcctgggttt tctactgctg
     8041 aggccattac gaatgtttcc ggacgcggtg tgggcatgga tgtcgtccgc acaaacgtgg
     8101 aaaagattgg cggcaaagta gaaattgaca gccgtcccgg aaagggcacc actttgcggt
     8161 tgcggattcc gttgacgctg gcgattgtgc ccgcactgat cgtcttcagc cgcggacaga
     8221 gcttcgccct gccgcagggc gcgctctccg aactggtcca tctttcagcc gagcaggcaa
     8281 agacccaggt ggaatggatg gaaggggctg cgctttacag actgcgcggt aagctgctgc
     8341 cgctcgtgtt tctcgactcg cttttaaagc agcgggaaga gcaggcgcgc gaaggcgagg
     8401 catatatcgc cgtactcaat gcggagggac ggcgctatgg actgattgtg gatggactgg
     8461 ccgatccgga ggaaatcgtc gtgaagccgc tttcttccgt gctcaaacag atcggattgt
     8521 tttccggcgc aacagtcctt ggcaatggag agatggcgct gattctggat ccgggggcga
     8581 ttgcgacgca ggcaaacatt ggcatggcgc tcgaagaaga tgcgcacgaa gtcatcgagg
     8641 ccgggacgcg aaatggggcc accgaatatc tgctgatgca gagcgggcaa gagcatgcgg
     8701 ctgtgcctct cgataaagtg ctgcggattg agcgcattcc gcgcgccagg atggagtggc
     8761 tgggcggagt tcccgtactg cgttttgaag gctcgctgtt gccgctgcgt gataccggca
     8821 gcgcaggcga agagatgacg gttgtggtct gccgtgatgg gtcacgccat gtgggagttg
     8881 cggtttcaca ggtgctggac gtggccgacg gaaggccgct ggaagaggcc gggacgcatg
     8941 cagcggcacg caacgtgacg ctgctgcatg aaaaggtaac gagcattgtc ccgctggagg
     9001 tgattccggc gctgcctgtt caaacgctgg aggtgacgca gtgagcgcga tcatgctggc
     9061 tgaagatgtc attgagatgt gttcggtgcg tgtgggcgaa acgttgttcg gtgttccggt
     9121 gatgcgggtc ctggagattc tgggcaagcc tgcaacccat ccggttccgc tggcgcctga
     9181 gtttatcggc ggactggcgc attaccgcgg cgaggtgctg acgacggttt cgctgcgcag
     9241 gctgttgaag atgcctccgt gcggatcgag cgaagatgtt ctggtctttg aaggcgagga
     9301 cggatatttc gggctgctgg tggatgaagt cagcgaggtg ctgacggttt ccccggcgga
     9361 cttcgagggc aatccgtcca cgctcgatga ccgcaggaag gcgctgtttg cagggaccta
     9421 caagctgaag gacgggctgc tggtgatgct ggacccggag cggctcgatc cggtgcgcct
     9481 gacggaaatg gatctgaggt ctgcatgaaa gcgttgattg tggacgactc gagctttatt
     9541 cggagctttt tgcgcgcgct gctggaaagc catgcactgg agtgcgtgga ggccgaaaac
     9601 gggcaggttg cgctggacat tctggccgga agtacgtttg gtctggcgct ggtggacgta
     9661 aacatgccgg tgatgaatgg tcttgaattt gtaaagaggc tgcgagggaa tcctgagcat
     9721 aaagacatga aggtggtaat ggtgacgaca gaggcagacc acgcttttat agaggctgcg
     9781 ctggaatttg gcgccgatga atatctgatg aagccattcg atgaagagtc cctgcttgaa
     9841 aaactccaga tggttggact gagacaggca taggtgtatg caagcacgtc ctgttcgtgt
     9901 cctcgtggtg gacgattctc tggtcatgcg cagtcttctg cgtatggtga tggcttctga
     9961 tccgacgatt gagcttgccg ggatggccgc cgacggcgtg agcgcgcttg atgctgttga
    10021 gcgcctgaaa ccggaccttg ttctgcttga tatcgagatg ccgcgcatga acggcattga
    10081 aatgctggca gaactgcggg cgcgaaagca tcctgtcaaa gtaattatgt gcagcacgct
    10141 cacgcggcgc ggcgcagcca tcacgctgga ggcgctggcg cgtggggcaa cagactacgt
    10201 taccaagcca acggcccaac acggcgcggc agacgcagtg gccacattga cgcgggagct
    10261 gctgccaaaa atcagaggtc tgttcccttc cggcaggaat gaaactgacc gagccgtcac
    10321 cagagaggcc caggctgagg gtcagcgtaa gccggccagc attgtggtca tcggcgtttc
    10381 gaccgggggg ccgacagcgc tggaagcgtt attgccgcag cttcctcagg agtttcctgc
    10441 tccgattgtg atagcgcagc acatgccgcg cctgtttact tccctgctgg cggagcggct
    10501 tgaccgcgtc tgtgatctga gggtgcgcga ggcagtggct ggcgagcgta tagcggcggg
    10561 atgcatttat ctggcgcagg gcgatcaaca tcttcagttg acgcgggccg cttttggcca
    10621 gggccatgtc ttccgtctgt ttccacctgc cgcaggtgat ttctgccggc cttctgtaga
    10681 tatgttgttc cggtcggccg ccgatgtatt gggcgatggc gttgtcggcg tggtgctgac
    10741 cgggatgggc tcggatggcc tggaaggatg cagggccatt cgcaaagccg gaggccaggt
    10801 gattgtgcag gaccgcgcca ccagcgcagt gtggggaatg cccggggcgg tcatggaagc
    10861 tggtctggca catcgtgttc tgccgctgcc ttcgattgcg gcggaactgg cgcgcgtgtg
    10921 cgccgtacca tccatatctg ttctcacgag ggccactgcc tgatgccatg ttctgagatt
    10981 gatctgacgt tcctgcgaca ctttctccag gagcgttccg gaaacgtcct cggccctgag
    11041 cgagaccact tgtttgaggc gcgactttat ccgatatggc agagcctggg gatgagcggt
    11101 ctcgatgagc tggttgaaaa gctacgcttc gccaccgatc cggcatttga gcaggcagtg
    11161 gtggaagcca tgaccatcaa cgagaccagc tttttccgcg accatgctcc gtttgacctg
    11221 ttacgggaag aactcctgcc aaagctcatc cgggcgcggg cagaggagcg gcggttacgc
    11281 ttctggagcg ctgcctgttc gagcggacag gaggcgtatt cgctcgcgat gctgttgcga
    11341 gagagctttc cacaggtcgc agactggaac attgaaattc tgggcacgga catccacgcg
    11401 gcgatggtgc ggcgcgccca cgcaggacgg tatcagcgca tggagatcaa ccgtggtctt
    11461 cccgcgcggt atctgctgaa gtatttccgg cgcgatggcg aagagtggga ggctgttccg
    11521 gaattgcgcg ctatgtgccg ctttgaacag cgaaatcttg cttacttttc gccgtttttg
    11581 acacaatttg acggtatttt gctgcgcaat gtgctgtttt atttctccga ggccaggcag
    11641 atgtcaatcc tgagaagtgt gcgcagttgt ctgtacccag acggtttcct tttgcttggt
    11701 cctagtgagc gggcggatat ctccggacag tggcggccct tctttcatgg aaatgtctgc
    11761 tattacaggc cgtgttgacc tcccctgggc gtttttgatg gctgtccgcc gggagcgtaa
    11821 tggggtaccc cctccccctc ttttgtacgg ggatgcttta ttttcaataa cttacaaggg
    11881 cgggtctgta cgggaatcgt ttattttcaa tgagttagct aagaagcggt cagcagccag
    11941 cgcctgcttc acaggctgtc agcccagagt ttcttctccc tttaggctct cttttccggg
    12001 gattcgtttc ccagatcctc tggcttccca tagataatcc aggtatatat aaaattcaga
    12061 tttaattttt ttgtaaattc tagaatcttt gagagttaga aaagtttagg ctctcatttc
    12121 tgactcattt tcatacctag attgtctatt tatgattctg attcttcata agggattcca
    12181 tggggcaccc ggctggatga tttatttttg ccctttcttt caggatatca agtggaaggg
    12241 agggatccgg acaatatcgg gtgggcctat cttattgaaa aatgacggat tcggtggatt
    12301 tattactttc gtaagggttg acagccggat ttttccttca gattccagtt tttacgatgt
    12361 gcatttcatt ctctggcttc taccggactc ctcctttact ccaggagggc ggtcccccag
    12421 cggctaaagc cgtgcccctt caaaacccga ttttctggcg gcggggaaaa tcttggcagg
    12481 attggctaaa cctgcatcgc tgagactctt cctcccttcg tgcgtcagaa tgacggggct
    12541 atatatgtgt cgtcggaatg acggactaca tagatcgcac atcggtcgtc agtaatatat
    12601 ctgttgtcag aatgacggac tctcctatat gggaaaggcc atatagtctt tcatttcagg
    12661 gcaacaaaat cagaacaggt ttccggaaat tatttgtttc aacagatgat gtttttattt
    12721 gagacttaca aaaacggccg tttcctccgt cctacactga agtgagacga tggctcggca
    12781 gacggcaaca gacgtccttg atttatttca tcccgtaacg gcggagtggt tccagacggc
    12841 actgggagag ccgactgccc cgcagcgcat gggatggcct gccattgcgc gcggcgagag
    12901 cacgctgatt ctggcgccga ccggaacagg aaaaacattg acagcatttt tgtggtgtct
    12961 ggaccggctt gtctttgggg agcgcgcgcg gggatgccgc gtggtctatg tgagtccgct
    13021 gaaggcattg gcggtggatg tggagcgcaa tctgcgcatg ccgcttgcgg gcattgcaga
    13081 gagggcgcag cgcatgggtg tggcgtttca tggtccggag atcagcgtgc gcacggggga
    13141 cactccacag aacgagcgca gccggttccg gcggcgtcct ggggaaattc tgattactac
    13201 gccggagtct ctgtatctgc tgctgacgtc ggagtcggcg gcggcactgc gcacagtcga
    13261 gacggtcatt gtggatgaaa ttcatgcgct ggtgccgacg aaacgcgggg cgcatctggc
    13321 gctgagtctg gagcggctgg aggcaatggt cgagcggccg ctccagcgga ttgggctttc
    13381 ggcgacacaa aga
//
LOCUS       DASRBV010000153         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_516131, whole genome shotgun sequence.
ACCESSION   DASRBV010000153 DASRBV010000000
VERSION     DASRBV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516131"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFA02_05860"
     CDS             <1..636
                     /locus_tag="VFA02_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZU09411.1"
                     /translation="AVILQWLLERKKWPGDVTRAFNTTKMLDRICARYGRTLHEHGIG
                     FKYVCDLMLEKDILIGGEESGGIGIKRHLPERDGLLNALLLANVMAEEGRTLGQLVSR
                     LQEEYGEHQYGRIDLHINDELKNATIAKARAGVKAFAGMPVERVETLDGIKFFLKNPE
                     AASKANAAETWLLLRASGTEPLLRIYSESCSKESVNKLLEAAREFAMNSLG"
     gene            780..2414
                     /gene="pgi"
                     /locus_tag="VFA02_05865"
     CDS             780..2414
                     /gene="pgi"
                     /locus_tag="VFA02_05865"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZU09412.1"
                     /translation="MATQVQSVPLTQLTAWKALEAHYQQIKDVHLRSLFADDPKRGER
                     FSQEAIGIYLDYSKNRITGETLRLLIQLAEESGLRQHIDAMFRGDKINVTENRAVLHT
                     ALRAPKDAVIHVDGKNVVPEVHEVLDRMAAFADRVRSGAWKGFTGKRIKNVINIGIGG
                     SDLGPVMAYEALRHYSQRDMVFRFVSNVDGTDFAEATIDLDPEETLFIISSKTFTTLE
                     TMTNAQTAREWSLKKLGDAKAVAKHFVAVSTNVAEVSKFGIDTANMFGFWDWVGGRYS
                     MDSAIGLSTMIAIGPDNFTKMLAGFHEMDEHFRNAPFEKNLPVLMGLLTVWYADFFGA
                     QTVAVLPYDQYLKRFPAYLQQLTMESNGKSVTLSGTRVSYQTGPIFWGEPGTNGQHSF
                     YQLIHQGTHLIPCDFIGFAKTLNPLGNHHDLLMANLFAQGEALAFGKTPEQVKAEGTP
                     DWLVPHRVFEGNRPSNTILLEKLTPEGLGKLVALYEHSVFTQGVIWQINSFDQWGVEL
                     GKVLAKNIISELEGKGEGKHDSSTSTLIARYRKLKG"
     gene            2566..2838
                     /locus_tag="VFA02_05870"
     CDS             2566..2838
                     /locus_tag="VFA02_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HZU09413.1"
                     /translation="MPTRNVNLTPEMDKFVSARIKSGQYANASEVVRAGLRALEREEQ
                     EEKAKLEALRRAIKEGDESGEFEGNPFDEVRKYIGELLSAKKVNVA"
     gene            2831..3127
                     /locus_tag="VFA02_05875"
     CDS             2831..3127
                     /locus_tag="VFA02_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZU09414.1"
                     /translation="MPEVRLSKRALRDFKSISHYTLETWGEAQAFRYVEDLELFLNQL
                     AQRPMLGRLCTDIASGLRRFEHGKHIIFYRPRRDGIFVVRILHRKMDPQKHISE"
     gene            3238..4368
                     /locus_tag="VFA02_05880"
     CDS             3238..4368
                     /locus_tag="VFA02_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZU09415.1"
                     /translation="MKKLRVLAIALAFATGVTGPFFFASNAVAQAARSDADIQSDVAY
                     ALTNSPQLQDQNITAATIHGDVTLSGTVRDEASKRLAEELVSRVNGVRSVTNNLTVSH
                     AAPQQTSPAPDQQQEEVPPPPPYNQGVSEPDQAENTQNNASPAQQTLNPVTIPAGTLL
                     TVRTSESLDARKLQIGSMFQATVANDIYSGNVLAIPRGAVVTGQVVNVQKPGDLAGKS
                     GLQLQITQLQLGGQTYSLMTDAWKADAPNKAGYSAGNTAGGAMLGAVIGAIAGGGPGA
                     AIGAVAGGTTGLAASAATKGPQVVIPPEALLNFHLANPVTVTPVSSQEAQRLAASAPA
                     PRQPRLVRRPVYVAAPYPYPYPYYYPYPYYYGPGFYYYHYWR"
     gene            complement(4584..>4782)
                     /locus_tag="VFA02_05885"
     CDS             complement(4584..>4782)
                     /locus_tag="VFA02_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZU09416.1"
                     /translation="NPIETPLLRMAREKGIPVVTGVEMFVHQGARQFEIWTGKPAPEE
                     EMFRVVVHALRQRAEEAKAGK"
BASE COUNT         1070 a         1231 c         1421 g         1060 t
ORIGIN      
        1 gccgtcattc tgcaatggct gctcgaacgc aaaaaatggc ccggcgatgt gacccgcgcc
       61 ttcaacacaa cgaagatgct cgaccgcatc tgcgcccgtt atgggcgtac gctccatgaa
      121 cacgggattg gatttaagta tgtctgcgac ctgatgctgg aaaaggacat cctgattggc
      181 ggtgaagagt cgggcggcat tggcatcaag cggcacctgc cggagcgcga tggcctgctg
      241 aacgcgctgc ttctggccaa tgtgatggca gaagagggcc gcacacttgg ccagttggtt
      301 tcgcgtctac aggaagaata cggggagcac cagtatgggc gcattgatct gcacatcaac
      361 gatgaactga agaatgcgac catcgcgaag gcccgcgctg gcgtgaaagc atttgccgga
      421 atgcctgtgg aacgcgtaga gacgctggac ggcattaaat tctttttaaa gaacccggaa
      481 gcggcatcga aggcgaatgc ggctgaaaca tggctgctac tgcgggcgtc ggggacagaa
      541 ccgctgcttc ggatttattc tgagtcgtgc tcgaaagagt cagtgaacaa gctgcttgaa
      601 gccgcgcgcg aatttgctat gaatagcctg gggtagggag cggtgggacc ttcccgccgt
      661 tcctgggttt ttacattctc acaggcactc cggcccttca ggacgcagaa tagatattcc
      721 atgttcggag agccagcgcg agaatctaac aggcagaagg agagaaagaa gggagcaaca
      781 tggccactca agtccaatcg gttccgttga cgcagttgac tgcatggaaa gcgctggaag
      841 cacactatca acagatcaag gacgttcatc tgcgctcgct ttttgccgat gatccgaaac
      901 ggggcgagcg cttttctcag gaagccatcg gcatttatct tgactactcg aagaaccgga
      961 tcaccggcga aacgctgcgg cttctgattc aacttgctga agagagcggt ctgcgccagc
     1021 acattgacgc catgtttcgc ggcgacaaga tcaatgtgac ggaaaaccgc gccgtgctgc
     1081 acactgcgtt acgcgccccg aaagatgcgg tcatccacgt ggacggaaag aacgtggtgc
     1141 ctgaagtgca tgaagtgctc gaccgcatgg ctgcctttgc cgaccgcgtg cgcagcgggg
     1201 cgtggaaggg ctttacgggc aagcgcatta agaatgtcat caatatcggc attggcgggt
     1261 ccgatcttgg tccggtgatg gcctatgaag cgctccgtca ttacagccag cgcgatatgg
     1321 tcttccgctt cgtgtccaat gtagacggga cggactttgc cgaagcgacc attgatctcg
     1381 atcccgaaga aacgctcttc atcatctcgt cgaagacctt taccacgctt gagaccatga
     1441 ccaatgcgca gaccgcgcgg gaatggagcc tgaaaaagct gggcgatgcg aaggccgtgg
     1501 caaagcactt tgtcgcggtt tcgacgaatg tggcggaggt gtcgaaattc ggcattgata
     1561 cggcgaatat gttcggcttc tgggactggg tcggagggcg ctattcgatg gattcggcga
     1621 ttggtctttc gacgatgatt gccatcgggc cagacaactt cacgaagatg ctggccgggt
     1681 tccatgagat ggatgagcac ttccgcaatg ctccgtttga gaagaacctg cctgtgctga
     1741 tgggtctgct tactgtctgg tatgcggact tttttggcgc acagaccgtt gccgtgctgc
     1801 cctatgatca atacctgaag cgttttccgg cttatttgca gcagctcacg atggagagta
     1861 acggcaagtc agtgactctg agtggaacgc gggtgagcta tcaaacgggg ccgatcttct
     1921 ggggagagcc ggggaccaac gggcagcact cgttttatca gcttatccat cagggaacgc
     1981 atctgattcc gtgcgatttt atcggctttg cgaagacgtt gaatccgctc ggaaaccacc
     2041 acgatctgct gatggcgaac ctttttgcac agggcgaggc actggccttt ggcaagacgc
     2101 cggagcaggt gaaggccgag ggcactccgg actggcttgt gccgcaccgc gtttttgagg
     2161 gaaaccggcc gtcgaacacc atcctgctgg aaaagctcac gccggagggg ctgggcaagc
     2221 tggtggcact ctatgagcac agcgtcttta cccagggcgt catctggcag atcaactcct
     2281 ttgaccagtg gggcgtggaa ttgggcaagg tgctagcgaa gaacatcatt tctgaactgg
     2341 aaggaaaagg cgagggcaaa cacgatagtt cgaccagcac gctgattgca cggtaccgga
     2401 agctaaaagg ttgaggctga ggggccggcc tttcgggccg cttttgcgcg aaaggatggg
     2461 cacccggaaa atcctgccat atttctggtg agatgtgggg ctccccattg tgtagtattg
     2521 ccaattattg ccaactgaat ttagcatgga ttccgaggtg atttcatgcc tacgcgcaac
     2581 gtcaatctga cgcctgaaat ggataaattt gtttctgcca gaatcaagag cgggcaatat
     2641 gccaatgcca gcgaagtggt gcgcgcggga cttcgcgcac tggagcggga agagcaggaa
     2701 gagaaagcga aactggaggc tttgcgtcgg gccattaaag aaggagatga aagcggggaa
     2761 tttgaaggta atccatttga tgaagtccga aagtacatag gagagttgct atcagccaag
     2821 aaggtgaacg ttgcctgaag tcaggctctc caagcgtgct ctgcgcgatt tcaaatctat
     2881 ttctcattac acgctggaaa cctggggtga agcacaagcc tttcgctatg tagaagatct
     2941 ggagttattt ctcaaccagc ttgcacaaag acccatgctg gggcggttat gcactgacat
     3001 cgcttcaggt cttcgccgct ttgagcacgg caaacatatt attttctacc gtcctcgccg
     3061 cgatggcatt tttgtggtcc gtattctgca tcgcaagatg gacccacaga aacatatttc
     3121 tgagtgaccc ctcccgtaac tttttcctgt caaacgtgtt tttataagcg acgcggaaac
     3181 acagcctacg tctatccaga aggtggcagg ttccgcgccg ctttcgaggt tgctgggatg
     3241 aagaagctac gggttttggc gattgcactt gcctttgcta ctggtgttac aggcccattc
     3301 ttctttgcgt cgaatgctgt ggcgcaggct gcgcgttctg acgctgatat ccagtcggat
     3361 gtcgcatatg cgctaacgaa ctctcctcag cttcaggacc agaacatcac cgctgcaacc
     3421 attcatggcg atgtgacgct ttccgggacc gtgcgcgatg aggcatccaa gcgcctggcc
     3481 gaagagctgg tatcgagggt gaatggtgta cgttccgtga cgaacaatct gacggtcagc
     3541 catgcggccc cacagcagac ttcgcctgct ccagaccagc agcaggaaga agttccgccg
     3601 cctccgccct ataaccaggg agtttcggag cctgaccagg cagagaacac acaaaacaac
     3661 gcgtctccgg cacagcagac tttgaacccg gtaacaattc cggcaggaac gctgctgact
     3721 gtgcgtacca gcgagtcgct cgatgcgcgt aagctgcaaa tcggctcgat gtttcaggcc
     3781 acggttgcca acgatattta ctcgggcaat gtgctggcga ttccgcgcgg cgctgtggtt
     3841 acggggcagg tcgtgaatgt gcagaagccg ggcgatcttg ccgggaagtc aggcttgcag
     3901 ttgcagatta cccagctcca gttgggcggg cagacgtatt cgctgatgac ggatgcgtgg
     3961 aaggcggatg ctccgaataa agctgggtac tcggcgggca atacggccgg cggggccatg
     4021 ctgggcgcgg tcattggcgc gattgccggg ggagggccgg gtgcagctat tggtgcggta
     4081 gccggcggga cgactggact tgcggcctcg gccgcgacga aaggtcctca ggtcgtaatt
     4141 cctccggaag cgctgctgaa ttttcatctg gcgaacccgg tgacggtcac gcctgtttct
     4201 tcccaggaag cacagcgtct ggcggcttct gcacctgctc cgcgacagcc gagactggta
     4261 cgccgtccgg tctatgtggc cgcgccttat ccttatcctt atccttacta ttatccgtac
     4321 ccgtactact atggaccggg attttattac taccactact ggcggtaaat cagagtttca
     4381 ttcagcttcg gtattctttc aagagcaggt ttgttgaaaa gtaactgaat gctgagaata
     4441 atgcacggct gctgcaaacc ggaaaagaaa cacacgtccc cagcctgtca tcagttatga
     4501 cagcatagga cggtatgaac gagtgttgtg gttcttcgct gggcttctca gaagggcagc
     4561 ggcttacaca aattcagcgc cagttatttg ccagccttag cctcttctgc gcgctgtcgt
     4621 aaggcatgca ccacgacgcg aaacatttct tcttccgggg cgggtttgcc ggtccagatt
     4681 tcaaactgac gcgcgccctg gtggacgaac atttccactc ccgtaacgac aggaatgcct
     4741 ttttcacggg ccatgcgcag cagcggtgtt tcgattgggt tg
//
LOCUS       DASRBV010000154        16658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_414012, whole genome shotgun sequence.
ACCESSION   DASRBV010000154 DASRBV010000000
VERSION     DASRBV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414012"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..966
                     /gene="rpsB"
                     /locus_tag="VFA02_05890"
     CDS             76..966
                     /gene="rpsB"
                     /locus_tag="VFA02_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZU09417.1"
                     /translation="MATITMKELLEAGVHFGHQTKRWDPRMKEYIFGERNGIYIIDLQ
                     KTLKMFKDAAKYVTDIAAQGKIILFVGTKRQAQDAIAEEANRCGMFYINNRWLGGLLT
                     NWVTVQKSVKRLQELDEMATDGRYDLLTKKEVIRLERERKHLQANLAGIKNMKRLPDA
                     LFVIDSNNEAIAVKEARKLGIPVVAVVDTNCDPTVVDYVIPGNDDALRAIRLFASKIA
                     DSVIEGVQLVGDKQFAEAVGGTTEAQTEGEAGTVENIEASTSDYEEDVDLEAALGGVI
                     RKSPSVVSSLDEAEAAESSY"
     gene            1094..1753
                     /gene="tsf"
                     /locus_tag="VFA02_05895"
     CDS             1094..1753
                     /gene="tsf"
                     /locus_tag="VFA02_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZU09418.1"
                     /translation="MSTVTEKIDAKLVKELREKSGAPMGDCLKALQEAKGNMEEAFVI
                     LRKRGMASAQKKASRTTNEGAVGTYIHAGGKIGVLVEVNCESDFVARTTDFQELLKDI
                     AMHIAATDPRYIRREDVTAEDLEREKDIFRAQAAETGKPAPVIEKIVEGKMSKFYEEV
                     CLLEQPFIKDQTVSIKDLIAQKVGKLGENITVRRFARFKVGDPNWTVAQTKAVAAEEQ
                     A"
     gene            complement(1962..4262)
                     /locus_tag="VFA02_05900"
     CDS             complement(1962..4262)
                     /locus_tag="VFA02_05900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HZU09419.1"
                     /translation="MTFTRSCGFLTAILVAGCGLYAQQKRPVDYVNGLVGTAPLDDQR
                     LIGNAPPPGELLYSGFTSPNAVLPHGSTDPGPINANLDLEYPAGVRAPYFYPNRMMYA
                     FTAGMRGGPIVMPVVGNWTPPPQRSASVYDKTKEISAPGYYSVYLDDFHTQAEMTATT
                     WTSLFRFTFPQSEKSHVLLDLGRGGGEIEIVDDHNVRGKSRYGSRYFVAEFSRPFTSY
                     GTFKQHPPDDPSHGDILGWDEVDPDAKTISGRFAGAYLRFTTKPGEQILVKIASGFSY
                     EMAEQHLQNEDPGWAFDRIRQQAEQAWAKKLDQIEVEGGTEKEKTLFYSCLYHSFASP
                     HLLARKGEQFIGEDHKLHTADYDRYDQVPLWDTGRNQVVLLTMLEPDTKVNILRSQLD
                     IARESGYMGTSFHGDNADLMFLGDWERGLHFDWAAAYEYLLKNATDPNGPRGHLAEYM
                     QKGWISDFIPKDQDPSPPYADGNAGVATTLEYSWDDHALALYAKKLGKEDDYEMFLKR
                     AHNYKNVFDPSIGFMRGRTEDGKWISPFDPREPYYNFMMKEASGWSTLWLVPHDVQGL
                     MDLLGGREKFAAKLDEFFTTPYTAKGICRDCTGVIGQYVQGNQPDQQAAYYYDWAGQP
                     WKTQELTRRILKLMYGSDYTGLAFPGMDDQGATSSWYVMSALGFYPVDPSSPDYIIGS
                     PIFNRAVIHMGNGKDFTIIARNNSEKNIYIQSATLNGKPWNKPWFSHSDIANGATLVL
                     TMGPSPNKSWGSAPEAAPPSMTQAGQ"
     gene            complement(4348..8100)
                     /locus_tag="VFA02_05905"
     CDS             complement(4348..8100)
                     /locus_tag="VFA02_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09420.1"
                     /translation="MRNGCIPASCRQPGKKYRAALLAIVLAFLVYPSHAQEFRSVLTG
                     LITDPSGALITNATVTAVNNASGTSYTAQTSSKGVYYIPYMLPGTYTVTVQANGFKTA
                     VQDKVLLLASQTFNQNFTLELGSVAQQVVVNTAPPQLETTTGSGGTVIDQRTLQSVPL
                     NGGQSYMLIGTTPGSQFTQTQFGTGGYSGTRGWDVTNSYTIGGGIVGNNQFTLNGTNI
                     TSQFGYDNHSPGEWTVSPNIDSIEEVNVMTTTYDARYGRTSGGTVNVVTKSGGDQYHA
                     SARYAYEGAFMNANTFQNNLAGSPRQGEVQNQWWITAGGPIIRRKLFFFFGFEGYRQS
                     LAGSTLLNVPPAYLRPGYNGNPGVDFSQVQKLDPQEFPNGLTIFQPGTATCLDGGSVT
                     NCQSNHVVQTPYPNNTLPGSQINATSQAVLKYIPLPNIPGTENLARGANYLAHTPDLY
                     NYNQPQVRVDYNLTDKTKLYSYFLYWKGNENRSQNGLTGIASHGNINWIHQNWVATQD
                     VTHVFSPTLVGDFKVAFDRFFESSPDGDFSHQTDPSTIGLAMPLPDTTSGKYLPEFNV
                     NDGWGTGFLSGNTVFGNQLNADVTNNYVFNADFTKTAGAHTLEFGGEIDEFQYGGRPY
                     SGGHPNGSFGFNSGWTQFNPHNGHCYPITPGNGNSNGCTSNTPNGSSLASFYLGLPSS
                     GGVDWIYSLMEGYPVYAVYFQDNWRVNHRLTLNLGLRYDVQRGLRERYNHLNRGMCLT
                     CVNPLTNDPAYQANVANAANIAAWQAAGINPGSLQQVLGGIQFAGSGGQSRDAYDTDW
                     SDVGPRIGFAFVIDPKTVVRGGWGLMYSYGLEGGSSVGEAQTTNYTASLDGGNTPTNY
                     FQTGQPFASGLIKPTGNSLGLLTDVGNGGVQVDFPNRRVPTEQILSFGIQRELPWAMV
                     LDARYAGNFSSRLRTFLWINGTATLAQLKAAQANPQIWDQQVPNPYFNVPGISGPGQC
                     GTSSTVQAIALVLPLSQYCSPGGTGLVGQYNAALGRQWYDGLEVKLTKRANHGLTFNM
                     AYTYSKTMNGNGYQNGWPYQDISQIHQIADTDRTHVFSLTSVWDLPFGKGGLVGANSS
                     RLVNSIIGGWTLSGVFNAQSGMPVGINTGWWYTCPGQSYRPKGGTSVGQGRWFNQDLS
                     CWQSIPEWGLMNLKGKTAELRNPMMPNLDASIQKSVPITEKLDFQLRLDAFNAFNSVL
                     FGGPNTDPGSGPASYSPTSGWSGFGTVGPTQQNFPRILQISGKISF"
     gene            8117..8302
                     /locus_tag="VFA02_05910"
     CDS             8117..8302
                     /locus_tag="VFA02_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09421.1"
                     /translation="MAKVILLWYGFHSGMVAIPPSRDTEVTVPEEQLAIRQMARLKMV
                     QMNYFSGHWHKGRKIDA"
     gene            8299..9918
                     /locus_tag="VFA02_05915"
     CDS             8299..9918
                     /locus_tag="VFA02_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09422.1"
                     /translation="MKAMFADTRLFQRWLLVAATGAVAAFIAFWVYRTYAGNTVPFHT
                     YALQTGSKDWTPIGGDWIITNGVVVNNSPERGAKLLTGSDYWQNLTFTTEMKFTGTNA
                     DMGVIIRVNVPRQGADSYNGYYIGLRTLDGTIVVGRSDFGWREAPPVHVPGGVVSNVW
                     YRLRVTAYGCNIAASVQNSVTLQTAWIAFRESGCVSSGRIGLRSLNAGGMWRNISIAP
                     ATWNDYLQLSQHATAVEYPEIVPGPPWWTPVHAGILFGGLLAFLLLLQLVYFRIQQWK
                     AYTITRERERLAHDIHDTMAQSFAGIGYQIQGIYHHMRCGGPVDSAEIADQLHVAYQL
                     VHRCHEEASRTIAMLGSSLPLTRQNILDALKETATKIAGGRITVVAQSHGTSARLNLR
                     LADALLHIGREAIVNAVDHSGLSTLEIVLSYNKTSVELAIKDNGCGFEFSQKTIGFGI
                     LGMQKRARDVGATLQILSRPGMGTRVKVSAGIHPENLIARIVAGIRRALSPSGIAFHL
                     LRKPLSRLSFRDAYQPSPDGSRYCFGAIIRT"
     gene            complement(9847..10494)
                     /locus_tag="VFA02_05920"
     CDS             complement(9847..10494)
                     /locus_tag="VFA02_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09423.1"
                     /translation="MARPLKPPTAIKVLIVDDHPVVRVGLRTMLESEENISVTGLAES
                     AAEAFREVERDPPDVVLMDLRMPEMEGIDAIAKLRRIHSDIRILVLTNYETDDYVFRA
                     LQAGAMGYLLKSTPQAEIVRAVEKVHDNQRCIPPEIQERLAEIIGREELTQREREVLG
                     LAAAGMTNKEIAQQLFISDKTARNHIASCLVKLGANDRTEAVTTAIRRGLIRVPE"
     gene            11069..14197
                     /locus_tag="VFA02_05925"
     CDS             11069..14197
                     /locus_tag="VFA02_05925"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09424.1"
                     /translation="MAHANDIYIAQSAAGANSGASCSSAHSVAWLNSSSNWGTGSAQV
                     GPGDTVHLCGVISSPITFYGSGTSSAPIKLFFESGASMQAPVWNPAAITLNGQSWLVI
                     DGGANGRIFDTNSGTGKIAQDSVCVLTDSNAANVTIQNIECGPLYVRTSSSDSNQLAS
                     GVSSVSGPNLTIRNSKFHDAYYCIDTGGPNASGLNVYNNDVYNCSTGIVLGDGGSGTT
                     ISNAQIHGNHIHDGANWDGCWNGCSIWNHNDGIHVWTSSGGKQNAPKVYGNTIDGSWG
                     AHTTAFIFMEADQGPMPSPMVYNNVLNNTGPNSPTNGLVEMKDNITSGQLYNNTFDCQ
                     TAGFNGIHWEGGSSSKALNNVFLNCGTAFANDDGTGTMSHDYTAYWNTPLQGDAHIVI
                     ANPLLSALLAPLAGSPLTAAGENLTSYFSTDITGATRPSSGGWTLGAYQATGTTTTSY
                     TLNVGTSGSGSGTVTSSPAGISCGTTCSTTYAAGTSVTLTASPDSNSTFAGWSGSCSG
                     TGACTVNMNSNASVSATFNPSSSGSSGGTSGGGSGSGTGSGSGSGNQISWVQGKPCVS
                     NSASSMTCGFSSLVTAHDLIVVGMSWTGSGSPVISDSLHSKFTQAVTRSWNNGAATSS
                     VYYAVAPAGGPDTLSISFGGTVSYPTVYVHEYKGADPNSPLDGATGSSGVASANPVTG
                     NTTASDSGELLFAYGDSLDYNMTAGSGFALRITSSFSAASQDAIVSKSGTYGATFGLP
                     GNTSWVALLASFKPATAAPVLTVTKNGAGSGTVTSLPSGISCGTKCSATYASGTKVVL
                     TANPASGSTFSGWSGACSGTGTCSVTLSASTSVTATFTPISSAKIAFVQSQSCIAGSG
                     TSIKCTFPANVASNDLLVIGTSWSGSASLSISDSLGSGWKQAISKTWNGSASTSRIYY
                     AVAPKGGSDTITLTFNGSVNYPILYIHEYHGENSSKPLDVVSSSAGTSSGNFNSGSAT
                     LTAPYDLVFGYADAVSNQVGAGPGYKRRGSSGFSAISEDALAVYAASTSATFTDPGPD
                     TWVALMAAFKAQ"
     gene            complement(14322..15194)
                     /gene="rsmI"
                     /locus_tag="VFA02_05930"
     CDS             complement(14322..15194)
                     /gene="rsmI"
                     /locus_tag="VFA02_05930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZU09425.1"
                     /translation="MPEAEERPAPGLYLVGTPIGNLEDITLRALRVLRSVDRIACEDT
                     RQTQKLLNHFGIATPTISCHEHNENERIPELVSALKQGARIAVVSDAGMPGISDPGMR
                     LVTAAIRGGIAVCPVPGANAAISALVASGLNTKRSFFAGFLPAKPGARRAELERLAAA
                     VPDETTLIFYEAPHRILETLSDVEIVWGPACRIVIARELTKLHEEFLRGTVSGVAAAL
                     KQRDRVRGEITLLVEARLKSEETRAVGISERLAELQQSENLDEKDALKRIARERGLSK
                     SDVYRQLQRERGKR"
     gene            15307..>16658
                     /locus_tag="VFA02_05935"
     CDS             15307..>16658
                     /locus_tag="VFA02_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09426.1"
                     /translation="MEHIFRYFAVSALTASLILGAAGCHKQSDQQAQDQTQDQNADPA
                     AANMAPVDSSQSTPTNEAETAPSRTPAATENTQAAQPSSQPSYSTETTPPPPDETASS
                     YPENTTYPEDTAVPTSDISPDQSGYYDDSAYQQPVYAAQPPPPIPVYIQPPCPGPNYI
                     WTPGHWAWSPMGYYWVPGAWVMAPYTGALWTPGFWLFVSIGHFFWHPGFWGPHIGYYG
                     GINYGNGYFGHGYDGGYWNHGNFYYNRSVTNVNTTIVKNVYVHNVTINNYNINRVSYN
                     GGPGGVQAHPLPAEMVAMREPHLPPLRVQTQHIQQAAQNRAQFFNVNHGRPQTVALER
                     PLATPFRAPAAQPPVRGVANPGFSARQQQHVTATPQFNRLEQHAGQQQAHRPEMLQPA
                     PTSQVRPQQPQQNRPNLIRPGTHPQAQQQQRPASPQRIEARPQEQEYRPAPQQRQETR
                     P"
BASE COUNT         3733 a         4562 c         4623 g         3740 t
ORIGIN      
        1 gcaagtaaat cttcccaaaa aggggaatca atcccggccc tgaactgtcg ggatgcaatc
       61 cacgaaggag gctgtttggc cacaatcacg atgaaggagc tgctcgaagc gggagtccac
      121 ttcgggcatc agacgaagcg ctgggaccct cgcatgaagg agtacatctt cggcgagcgc
      181 aacggcattt atatcattga cctgcagaag accctgaaga tgttcaagga cgcggccaag
      241 tatgttaccg acatcgccgc ccagggaaaa atcattctgt ttgtcggtac caagcggcag
      301 gcgcaggacg ccattgccga agaagccaac cgctgcggca tgttctacat aaataaccgt
      361 tggctgggtg gtctgctgac aaactgggtg accgtgcaga agtcggtgaa gcgccttcag
      421 gaactggatg agatggccac agacggccgc tacgacctgc tgacgaagaa agaagtgatt
      481 cgtctggagc gcgagcgcaa gcaccttcag gcgaacctcg caggcatcaa gaacatgaag
      541 cgtctgccag atgcgctctt cgtgattgat tcgaacaacg aagccattgc cgtgaaggag
      601 gcccgtaagc tgggcattcc cgtggtcgcc gtggtggaca cgaactgcga cccgacggta
      661 gtggactatg tgattccggg taatgatgac gcgctgcgcg ccatccgatt gttcgcttcc
      721 aagatcgcgg actccgtgat cgaaggcgtc cagttggttg gcgacaagca gtttgccgaa
      781 gccgtgggcg gcacgacaga agcgcagacg gaaggcgaag ctggcactgt cgagaacatc
      841 gaagccagca cttcggacta tgaagaagac gtggacctcg aagcggctct gggcggcgta
      901 atccgcaagt cgccgtctgt ggtcagttcg ctggatgaag ctgaggcggc ggaaagcagc
      961 tactaagacc tgggcaggta cgggtatctg aaagcgtggc tgcggttgaa aagggccacg
     1021 cttttgttgt gtctggggca aatgctttgt ttacgatgac aaagttttta ggaaaaagat
     1081 tggggaaaat ggaatgtcaa ctgtgactga gaaaattgat gcaaagctgg taaaggaact
     1141 gcgcgagaag tcgggcgcgc ccatgggcga ctgtctgaag gcgctccagg aagccaaggg
     1201 caacatggag gaggcgtttg tcatcctgcg taagcgcgga atggcttcgg cccagaagaa
     1261 ggcttcgcgc acgacgaacg agggtgcggt cggaacctac atccacgctg gtggcaaaat
     1321 cggggtgctg gtggaagtga actgcgagag cgatttcgtg gcccgaacta cggacttcca
     1381 ggagcttctg aaggacatcg ccatgcatat tgcagcgact gatcctcgct atattcgccg
     1441 cgaagacgtg acggcagaag acctcgagcg cgagaaggac atcttccgtg cacaggccgc
     1501 tgagacgggc aagcccgctc cagtgatcga gaagatcgtt gaaggcaaaa tgagcaaatt
     1561 ctacgaagaa gtttgcctgc ttgaacagcc cttcattaag gaccagaccg tttcgatcaa
     1621 ggacctgatt gcgcagaagg tcggcaagct cggcgagaac atcactgtgc gccgcttcgc
     1681 ccgtttcaag gtcggtgacc cgaactggac ggtagcacag acgaaggccg tcgcagcaga
     1741 agagcaggca taaacccact tcagtcatcg aacccaaaga cccggccaat gcgccgggtc
     1801 ttttactgtt cataaaaata aggattgcca ttccgactga gaagggggcc taagccatga
     1861 ccggtcgcgg acttaacggt ggggacctat gcaaggttcg caaccgatca gatcttcgat
     1921 gaacattcct gagggctccc gttccacaag ttcgtggcct ctcactgtcc ggcctgtgtc
     1981 atagaaggag gtgcagcctc aggtgcgctg ccccagctct tattgggaga gggtcccatc
     2041 gtgagcacca gtgttgctcc gtttgcgata tcagaatgac tgaaccaggg cttgttccac
     2101 ggtttgccat tcagggttgc agactggata tagatgttct tttcggagtt gtttcgggca
     2161 atgatggtga agtctttgcc gttgcccatg tgaatgaccg cccggttgaa gatcggactg
     2221 ccgatgatgt agtccgggct ggacgggtca accggataaa atcccagcgc gctcatgaca
     2281 taccacgatg aagtcgcgcc ctggtcatcc attcccggaa atgccagccc tgtgtagtcg
     2341 ctgccataca tcagtttcag gatgcggcgc gtcagttcct gcgtcttcca cggctgtccg
     2401 gcccagtcgt aataataggc cgcctgctgg tcaggctggt taccctgcac gtattggcca
     2461 atgactccgg tgcaatcgcg acagatccct tttgcggtat atggagtcgt gaagaattca
     2521 tccagcttgg cggcgaactt ctctctacca cccagcaaat ccatcaggcc ctgcacatca
     2581 tgaggaacca gccaaagagt agaccatccc gaggcctcct tcatcatgaa gttgtagtat
     2641 ggctcacgcg gatcaaaggg cgaaatccat ttgccatctt cggtcctgcc acgcataaaa
     2701 ccaatcgaag gatcaaagac gtttttgtaa ttgtgtgcgc gcttcaggaa catttcatag
     2761 tcatcctctt tgccgagctt ctttgcatag agcgcgagtg cgtggtcgtc ccaactgtat
     2821 tcgagcgtgg tggcaacccc ggcatttccg tcagcatacg gcgggctggg gtcctggtct
     2881 ttgggaatga agtcagatat ccagcccttc tgcatgtatt cggcaagatg tcctctgggg
     2941 ccgtttgggt cagttgcatt cttaagcagg tactcatagg ctgcggccca gtcgaaatgc
     3001 agtccgcgct cccagtcgcc cagaaacatc aggtcggcgt tgtcaccatg aaatgaagtg
     3061 cccatgtagc cggattcacg cgcgatgtcg agttgcgagc gcaggatatt gaccttcgtg
     3121 tccggctcca gcatcgtgag cagcacgacc tgattgcggc ctgtgtccca cagcggcacc
     3181 tggtcgtagc ggtcgtagtc cgcggtgtgg agtttgtggt cctcgccgat gaactgctcg
     3241 cccttgcgcg ccagcaggtg cggactggca aaggaatgat agaggcagga gtagaacagt
     3301 gtcttttcct tttctgttcc gccttcgact tcaatctgat cgagcttttt cgcccatgcc
     3361 tgctctgcct gttgacggat gcggtcgaat gcccagcccg gatcttcgtt ctggaggtgt
     3421 tgttccgcca tttcgtagct aaagcccgag gcgatcttca caaggatctg ttcgcccggc
     3481 tttgtagtga agcgaagata cgcccctgca aaacgtccgg aaattgtctt tgcgtccgga
     3541 tctacttcat cccagccaag gatgtctcca tgagatggat cgtcgggtgg gtgctgtttg
     3601 aaagtgccat agctagtgaa aggccgcgag aattctgcga cgaaatagcg gctgccgtag
     3661 cgcgattttc cgcgaacgtt atggtcgtct acgatttcga tttcaccacc gccacggcca
     3721 aggtcgagca ggacatgcga tttttctgat tgaggaaagg tgaagcgaaa gagagatgtc
     3781 caggtagtgg ctgtcatctc ggcctgggtg tggaagtcat cgagatagac gctgtagtat
     3841 ccgggtgctg atatttcctt tgttttgtca tagacagaag cgctgcgctg cggcggaggg
     3901 gtccagttcc cgaccactgg catcacaatt ggaccgccgc gcattcccgc ggtaaaggca
     3961 tacatcattc tgtttggata gaaatacggc gcgcgcacgc ctgcgggata ttcgaggtcg
     4021 aggtttgcat tgattgggcc tgggtccgtt gagccatgcg gcaggactgc attgggagaa
     4081 gtaaagcctg aatacagcaa ctctcccggt ggcggcgcgt tcccaatcaa cctctggtca
     4141 tcgagcggcg cagtgcctac caggccattc acataatcta cgggccgttt ctgctgagca
     4201 tacaatccac atcctgccac gagtatggca gtcagaaatc cgcaggagcg cgtaaacgtc
     4261 atgatacctc tgttcaattc atcgaaatga tatcgtcttg caaaaagaaa aagtcccttg
     4321 cctgggcgcg gcaagggact ttgtttgtca gaagctgatc ttccctgaga tttgcagaat
     4381 tctcgggaag ttctgctgcg ttggcccgac agtgccgaag ccgctccaac cagaagttgg
     4441 actatagctt gcagggccag agccgggatc ggtatttggc cctccaaaca ggaccgagtt
     4501 gaaagcatta aaagcatcca gacgaagctg gaagtccaac ttttcagtaa tgggcacgct
     4561 cttctgaatg gacgcatcca ggttcggcat cattggattg cgcaattcag ccgtctttcc
     4621 tttcagattc atgaggcccc attccggaat actttgccag caactcaagt cctgattgaa
     4681 ccagcggccc tgacctacag aagtgccgcc ttttggacgg tagctctggc caggacaggt
     4741 ataccaccaa cccgtattga tcccgaccgg cattccgctc tgggcgttga agacaccgct
     4801 gagtgtccag ccgccgatga tggaatttac caggcgagag gaatttgctc cgaccagacc
     4861 gccttttccg aagggcaggt cccagacact cgttaaagaa aagacatgcg tacggtcggt
     4921 atctgcaatc tgatgtatct gagaaatgtc ctgataaggc caaccgttct gataaccatt
     4981 gccattcatg gtctttgaat atgtgtaagc catattgaac gtgaggccgt ggttggcgcg
     5041 cttggtcagc tttacttcca gcccgtcata ccactggcgt cccagtgctg cgttatattg
     5101 cccaaccaga ccagttccgc ccggcgagca atactgcgac aacggcaaca ccagagcaat
     5161 ggcctgcacg gtcgaggaag ttccgcactg tcctggaccg gagattcccg gaacattgaa
     5221 gtacggattt ggcacctgct ggtcccatat ctgcggattg gcctgcgctg ccttcagttg
     5281 ggcgagagtg gccgtgccgt tgatccagag gaacgtgcgc agacggctgc tgaaattacc
     5341 tgcataccgg gcatccagga ccattgccca cggcagttcc ctctggatgc caaaagagag
     5401 gatctgctct gttgggaccc ggcggttcgg gaagtctacc tgtacgccgc cattgcctac
     5461 gtctgtcagc aatccgagac tgttgcccgt gggtttaatc aggccgctgg caaatggctg
     5521 tccggtctgg aaatagttgg tgggtgtatt gccgccgtca agagatgcag tgtaattggt
     5581 ggtctgtgct tcgcctacgc tggagccgcc ctctaaccca taggagtaca tcaggcccca
     5641 tccgccgcga acgaccgttt tgggatcaat cacaaacgca aaaccgattc tcggacctac
     5701 gtcggaccag tcggtatcgt aagcatcacg cgactgcccg cctgagcccg caaactggat
     5761 gccgcccagt acctgctgca gggaacccgg attgatgccg gccgcctgcc atgcagcaat
     5821 attggccgca ttcgcaacat tggcctggta tgccggatca ttcgtcagcg gattgacaca
     5881 ggttaggcac atgccgcgat tcaggtggtt gtagcgctca cgcaaaccac gctgcacgtc
     5941 atagcgcagc ccgaggttca gggtcaggcg atgattgacg cgccagttgt cctggaagta
     6001 gaccgcgtaa accggatacc cctccataag ggaataaatc cagtcaacgc cgccgctgga
     6061 tggaagtccg aggtaaaaag acgccagcga agaaccattg ggagtgttcg atgtgcatcc
     6121 gttagagttc ccattgccgg gagtaatggg atagcagtgt ccgttatggg gattgaactg
     6181 tgtccatccg gagttgaacc caaaactgcc attgggatgt ccgccactat aggggcggcc
     6241 gccatactgg aattcatcaa tttcgccccc gaattccaga gtatgtgcac cggctgtttt
     6301 ggtgaaatct gcgttgaaaa catagttatt cgtaacgtcc gcgttcaact gattgccaaa
     6361 aacagtgttt ccgctcagaa acccggtccc ccaaccatca ttcacgttga actccggaag
     6421 atattttcct gaggtcgtgt cgggcagagg catggccagt ccaatcgtgc tgggatcggt
     6481 ctgatgcgaa aaatctccgt ccggggaaga ttcaaagaaa cgatcaaatg caactttaaa
     6541 gtcgcctacc agtgtcggcg aaaagacatg cgtcacatcc tgggttgcta cccagttctg
     6601 gtggatccag ttgatgtttc cgtgtgaggc gattccagtc aatccgttct gactgcggtt
     6661 ctcattgcct ttccagtaca ggaaatagct atacaacttt gtcttgtctg tcaggttata
     6721 gtccacgcgg acctgcggct gattgtagtt atagaggtca ggggtatggg ccaggtagtt
     6781 tgcgccgcgt gccaggtttt ccgtgcccgg aatattgggc agcggaatgt actttaatac
     6841 tgcctgtgag gtggcgttaa tctgcgaacc gggaagggtg ttgttgggat atggcgtttg
     6901 cactacatgg ttactttggc aattcgtcac agagccgccg tcgaggcagg tcgctgttcc
     6961 cggttggaag atcgtaagcc cattcggaaa ctcctgcgga tccagctttt gtacctggct
     7021 gaagtccact ccgggattcc cgttataacc cggtcttaaa tatgcgggag gaacattgag
     7081 cagcgtggat cctgctagag actgccggta gccttcaaat ccaaagaaga aaaagagctt
     7141 cctgcgaatg atgggtccac ctgccgtgat ccaccactgg ttctggactt ccccctgacg
     7201 tggtgaacct gcgagattgt tctggaaggt attcgcgttc atgaacgcgc cttcgtatgc
     7261 gtaccgggcc gaagcgtgat actggtcgcc accgcttttg gtcaccacat tgaccgtgcc
     7321 gccactggtc cggccatagc gggcatcgta tgtggtggtc atcacgttga cttcctcaat
     7381 gctgtcaatg ttcggcgaga ccgtccattc gccggggctg tgattgtcat agccgaactg
     7441 gctggtaata ttggtcccgt tcagcgtgaa ctgattgttt ccaacaatgc cgccgccgat
     7501 ggtgtaggaa ttggttacgt cccagccacg cgtgccagaa tatcctccgg taccgaattg
     7561 cgtctgtgta aactggctgc caggcgtcgt cccgatcagc atgtaagact ggccgccgtt
     7621 cagtgggacg ctctgcaatg tgcgctgatc aatcacagtt ccgcctgatc cggtggtcgt
     7681 ttccagttgc ggaggagcag tgttcaccac cacctgttgg gcgaccgacc caagctcaag
     7741 agtgaagttt tgattgaagg tctgagaagc aagcaacagg accttgtcct gtacggcggt
     7801 cttgaaccca ttcgcctgaa cagtgaccgt gtaagtccca ggcagcatgt agggaatgta
     7861 gtaaacgccc ttgctgcttg tctgcgcggt atagctggtg ccggaagcat tattgaccgc
     7921 cgtcaccgtg gcgttggtga tgagtgctcc ggaagggtct gtgataagtc ctgtcaaaac
     7981 ggagcggaac tcctgtgcat gggatggata aaccaggaaa gcaaggacaa tggctagcag
     8041 tgcggcccgg tatttctttc cgggttgtct gcaggatgcg ggaatacagc cgttgcgcat
     8101 gggcgcctcc taatgattgg ccaaggttat cttgttgtgg tatggattcc attcaggaat
     8161 ggtagcgatt ccaccaagcc gtgacactga agttactgtt cctgaagaac aactcgcaat
     8221 ccgccagatg gcccgtttaa aaatggttca aatgaactat ttttctggac actggcataa
     8281 aggcaggaaa atagacgcat gaaagccatg ttcgcagaca cacgattgtt tcagcgatgg
     8341 ctactggttg ccgcaacagg cgccgtggct gcattcatcg ctttctgggt ctacaggact
     8401 tacgccggca acacagtgcc tttccacacc tacgcattac agaccggtag caaagactgg
     8461 actccgattg gcggggactg gatcattacc aatggtgtgg tcgtaaacaa ctctcctgaa
     8521 cgcggcgcca agttactgac cggttctgac tattggcaaa acctgacatt cacaacggag
     8581 atgaaattta ccgggacgaa tgccgatatg ggtgtcatca ttcgtgtcaa tgttcccagg
     8641 cagggggcag actcatacaa cggctattac atcggcctcc gcacgctcga tggaacgatc
     8701 gtggttggcc gatccgactt tggatggcgt gaggcgcccc ccgtccatgt acccggcgga
     8761 gttgtatcga atgtctggta caggttgcgt gtgaccgcct atggatgcaa tatagctgct
     8821 tccgtgcaga attctgtcac tttgcagacc gcgtggattg catttaggga atcgggctgt
     8881 gtttcgagtg gccgcatcgg gctgcggtca ttaaatgcag gtggaatgtg gcgcaacatc
     8941 agcattgcac ccgcaacatg gaacgattat ctgcaactaa gtcaacatgc gactgcggtc
     9001 gaatatccgg aaatcgtccc cggcccgccg tggtggacgc cagtgcatgc aggaattctc
     9061 tttggtggcc tcctcgcctt cctcctgctg ctgcaacttg tctattttcg tatccagcaa
     9121 tggaaggcct acaccatcac ccgtgaacgc gagcgcctgg cgcacgatat tcacgacacc
     9181 atggcgcaga gctttgccgg aatcggatac cagatccagg gaatctatca tcacatgcgg
     9241 tgtggcggac cggtagactc cgccgaaata gcagaccaat tacatgtcgc ctatcagctt
     9301 gtacacaggt gccatgaaga agccagcagg accatcgcca tgctcggttc ctcccttcct
     9361 ttgacccggc agaatattct ggacgcgctg aaagaaacgg caacaaaaat tgcaggagga
     9421 agaatcacgg tggtcgccca gtcccatggg acgtctgctc ggctgaacct tcgtctggcc
     9481 gatgctctgc tccacattgg ccgcgaggcc attgtgaacg cggtggacca ttcgggcctt
     9541 tccacgctgg aaatcgttct cagttacaac aaaaccagtg tggagcttgc gattaaagac
     9601 aatggatgtg ggttcgaatt cagccagaag accatagggt ttggcattct tggcatgcag
     9661 aaacgggcgc gggatgttgg tgctacactg caaatcctca gcaggccagg catgggcacc
     9721 agggtaaagg tatcggcagg aattcatccg gaaaatctta ttgcgcgtat tgtcgcagga
     9781 atcaggcgtg cgttgtctcc gtctggcatt gccttccatc ttctgagaaa gccactgtcg
     9841 cgcctctcat tccgggacgc gtatcaaccc tcgcctgatg gcagtcgtta ctgcttcggt
     9901 gcgatcattc gcacctagct tcaccagaca gctcgcaata tggttgcgcg ctgttttatc
     9961 gctgataaag agctgctgtg ctatctcctt attggtcatg cccgctgctg caagccccag
    10021 gacttccctt tcacgttgcg tcagttcttc ccgcccgata atttcagcca ggcgctcctg
    10081 aatctcagga ggaatgcagc gctggttatc atgcaccttt tcgacggccc ggacaatttc
    10141 cgcctgcgga gtgcttttca acaaatagcc catggcacct gcctgaagcg cgcggaagac
    10201 ataatcgtct gtctcataat ttgtaagcac cagaatgcga atgtcagaat ggatacggcg
    10261 cagctttgcg atggcgtcta tgccttccat ctccggcatg cgcaggtcca tcagaacaac
    10321 atctggtgga tctcgctcca cttcccggaa cgcctctgcc gctgattctg ccagtcccgt
    10381 gacactgatg ttttcttcac tctccagcat cgtccgcagt ccaacgcgga cgaccggatg
    10441 atcatccaca atgagcacct taatggcggt aggaggctta agaggccggg ccatgctgat
    10501 aattttatcg tccaggttgc tggaccaata tttccagaaa agtaacagat gctgatatga
    10561 acagattctg aatcagtcac cgtgttagta acaaatcagc gtattttttt cctgctcttt
    10621 gtaatagcta catattttcc ggggaagggc atagttataa gtgattgaat aatttatgga
    10681 tagagggtga atatgcattg gtagcctgct tgcatgcgga attgcaaatc ccgaaatttc
    10741 atagaaaggc agtgatctcc cccatgaata cgatccaact gctcgtatcc gacgcgggtc
    10801 ctcctgacac cgttttcata taaattcatc atcttgccga taaccaatgc atcggtatgg
    10861 atggggagaa aatccttctt ccactcgctc ttctgctctt gttgtctccc ccttccgtta
    10921 aagtttcacg tttctcagtt ttagcttgga ccagcagttt caattgttga tgtttctgcg
    10981 ttgctgtgta tttcgagcga gaggtttgta gtgcatgttg tttaaaaaat tattctcagt
    11041 tatatgtttt ctctttgcga caactctgtt ggcccatgcc aacgacatct atattgcgca
    11101 aagtgccgcc ggcgccaact cgggtgcaag ctgtagcagc gcacattccg tggcgtggct
    11161 caattcatct tccaactggg gtacgggatc ggcgcaggta gggcctggag atacagtcca
    11221 tctgtgcgga gtcatcagct cccctattac gttctatgga tcgggcacaa gttctgcgcc
    11281 gatcaagctt ttttttgaga gcggcgcttc catgcaggcg cctgtctgga acccggcggc
    11341 cattacgctg aatgggcaga gctggcttgt aattgatggc ggcgcgaacg gccggatttt
    11401 tgacacaaat tcgggcaccg gaaagatcgc ccaagacagt gtatgcgttt tgacagactc
    11461 gaacgcggcg aatgtcacga tccagaacat tgaatgcggg ccgctctatg tgaggaccag
    11521 ttcgagcgac agcaatcaac tggcctcggg tgtatcgtcc gtaagcgggc cgaatctcac
    11581 catccgcaac tccaaatttc atgacgccta ctattgcata gataccggcg ggccaaacgc
    11641 cagtgggctg aacgtatata acaacgatgt ctacaactgc tcaaccggca tagtgctggg
    11701 cgatggaggc agcgggacca ccatcagcaa tgcgcagatc catggcaacc atattcacga
    11761 cggcgcaaac tgggacggct gctggaacgg ctgctcaatc tggaaccata atgacggaat
    11821 tcatgtatgg acctcttcgg gaggcaagca gaatgctccg aaggtctatg gcaacacgat
    11881 agatggcagt tggggcgcgc atacaacggc gtttatcttc atggaggccg accagggccc
    11941 catgccttcg ccgatggtct acaacaatgt gctgaacaac acaggaccga actctccgac
    12001 aaacggcctg gtggagatga aagacaatat tacatcggga cagctttata acaatacatt
    12061 cgattgccaa actgcaggct tcaatggaat ccactgggag ggaggctcat cgagcaaggc
    12121 cctcaacaac gtcttcctga attgcggaac ggcttttgcc aatgatgacg gtaccggcac
    12181 gatgtctcac gactacaccg cttactggaa cacgcccctg cagggggatg cccacattgt
    12241 catcgccaat ccgttgctct ctgcattgct ggccccgctt gccggttctc cactgactgc
    12301 ggcaggcgag aacctcacct cgtacttctc aacggacatc acaggtgcga cgcgtccttc
    12361 atctggcgga tggacactcg gcgcatatca ggcgacgggt actaccacga ccagctatac
    12421 gctgaacgtg ggcaccagtg gttccggttc cggcacggtg acgagctcgc cggctggcat
    12481 ctcctgcggc accacatgca gtacgaccta tgcagccgga acatcagtga cgctgacggc
    12541 ctccccagat tcaaactcca cttttgccgg gtggagcggc tcctgctcgg gcacaggagc
    12601 gtgcaccgtc aatatgaaca gcaatgcttc cgtctcggcc acgtttaacc cgtcatcgag
    12661 tggcagctca ggcgggacct cgggtggcgg ttctggctcg ggtaccgggt ctggttctgg
    12721 gtcaggaaac caaatttcgt gggtgcaggg aaagccttgt gtgtcgaatt cggcatcctc
    12781 catgacctgc ggtttctcaa gtctggtgac tgcgcatgac ctgatcgtag ttggtatgtc
    12841 atggacaggc agcggatctc cggtgatttc cgattcgctg catagcaagt tcacccaagc
    12901 agtaacacgc tcgtggaaca atggcgcggc cacatccagt gtctattatg ctgttgctcc
    12961 tgcgggaggc ccggatacgc tgagcatttc gtttggagga acggtcagct atccaacggt
    13021 ctacgtgcat gaatacaaag gcgcggaccc gaattctccg ctggatggtg caaccgggag
    13081 ttcgggcgtg gcctctgcaa atcccgttac cgggaataca accgcttctg attccgggga
    13141 gcttctgttt gcctacggag acagcctgga ctacaacatg acggcgggca gcggatttgc
    13201 cctgcgcatt acaagctcgt tctcagcggc ttcgcaggac gccatcgtct caaagagcgg
    13261 tacttacggc gccacctttg gcctacctgg aaatacaagt tgggtggcgt tgctggcttc
    13321 ctttaaaccg gcaactgcag caccggtact cacagtcaca aagaatggcg ccggcagcgg
    13381 cacagtcaca agcctgccga gtggaatctc ctgcggaacc aaatgctcgg caacctatgc
    13441 gtccgggacc aaagtcgttc tgacggcgaa tcctgcgagc ggttccacgt tttccggatg
    13501 gagtggcgca tgcagtggca ccggcacctg ttcggtcacg ctgtcagctt cgacatcggt
    13561 aactgccacc tttactccaa tctcttcggc gaagattgca tttgttcagt cgcagagttg
    13621 catcgcaggc tccgggacct cgataaaatg caccttcccg gccaacgtcg ccagcaacga
    13681 cctgctggtg attggcacaa gctggagcgg gtcggcatca ctgagcataa gcgattctct
    13741 gggctcgggc tggaagcagg ccattagcaa gacgtggaac ggttcagcct ccacttcacg
    13801 tatctactac gcggtggccc cgaagggcgg ttcggacaca attacgctca ccttcaatgg
    13861 tagcgtgaat taccccatcc tctacatcca cgaataccac ggagaaaact cttccaagcc
    13921 tctggatgtc gtcagctcgt cggccggcac cagctcgggt aacttcaaca gcggttcggc
    13981 tacgcttaca gcaccgtatg atcttgtgtt tgggtatgcg gatgcagtgt caaaccaggt
    14041 aggcgcaggt ccgggctata aacggcgggg aagcagcggc ttctctgcta tttcagagga
    14101 tgctctggct gtatacgcgg cgagcacgtc ggccacattc accgatccag ggcctgatac
    14161 ctgggtcgcg ctcatggctg cgttcaaggc gcaataaaac agcgcttctc accgcggaac
    14221 gcgcagtttc gcgttccggg tgagaggcca acccaggccc gcagtggttc ggtgcccaac
    14281 ctttcgcgct gattttgcgg aaagggtggg agggcacgga gtcatcgttt gccgcgttcg
    14341 cgttgcagct ggcggtaaac gtcgcttttt gataggccgc gctcgcgtgc gatgcgtttt
    14401 agcgcgtctt tttcgtctag attttctgat tgctgcaatt cggccaggcg ctctgaaatg
    14461 ccgactgctc tggtttcttc agatttcagc cgcgcttcca ccagcagcgt aatctctccg
    14521 cgcacacgat cgcgctgctt tagcgcggct gctacaccgc ttaccgtgcc gcgcaggaat
    14581 tcctcatgca gcttggtcag ctcgcgcgcg atgacgatgc ggcaagccgg tccccacaca
    14641 atttccacat cactcagcgt ttccaggatt cggtgaggcg cttcataaaa gataagcgtt
    14701 gtttcatctg gaacagcggc ggcaagccgc tccagttccg cgcggcgcgc gcctggtttc
    14761 gcaggcagaa aaccagcaaa gaaggaccgc ttcgtattca atcctgaagc aacaagcgcg
    14821 ctgatggctg cattcgcgcc cggaacagga cagacagcaa tgcctcccct gatggcagca
    14881 gtaacaagac gcatcccagg gtcagaaatt ccgggcatac ccgcatctga aactactgca
    14941 atgcgcgcgc cttgctttag agcactgaca agctccggaa tgcgctcgtt ctcattatgc
    15001 tcgtgacaac tgattgtagg cgtggcaatg ccaaagtggt tgagaagttt ttgtgtctgg
    15061 cgcgtgtcct cgcaggcaat gcggtctaca gaacggagca cgcgcaaggc gcgcagcgta
    15121 atgtcttcga gattaccgat gggcgtgccg acaagataga gtcccggagc gggcctttct
    15181 tctgcttcag gcataagtat tcttacgcct acggtatacc gatgaaacgc atagcgcaaa
    15241 gtatgctgtt tatgcctgta ccacgacttt ttacccaaaa gtgcatctaa gccttcggag
    15301 caagagatgg aacatatctt cagatatttc gcggtatctg ccctgactgc cagcctcatt
    15361 ctgggcgcag ccggatgcca caagcagagc gaccagcaag cgcaggacca gacacaggac
    15421 cagaatgccg atcctgcggc tgccaacatg gccccggtgg attcatcgca atctaccccg
    15481 acaaatgagg ccgaaacagc cccatcccgc acgccggctg caacagagaa cacacaggca
    15541 gcgcagccgt cttcgcaacc ttcctacagc actgagacga cgccgcctcc accggatgaa
    15601 actgcgtctt catatcctga aaacacgaca tatcctgaag atactgcggt tccaaccagc
    15661 gatatcagcc ctgaccagag cggctattat gatgactcgg cataccagca gccagtgtat
    15721 gcagcgcagc cgcctccacc gattccggtc tatattcagc caccctgccc aggtcccaac
    15781 tatatctgga cacctggaca ctgggcgtgg tcacccatgg gctattactg ggtccccggg
    15841 gcctgggtca tggcgcctta taccggcgca ttatggacgc cgggattctg gcttttcgtt
    15901 tccattgggc acttcttctg gcatcccgga ttctgggggc cgcatattgg ttactacggc
    15961 ggcatcaatt atggaaacgg ctactttgga cacggctatg atggtggcta ctggaaccac
    16021 ggcaactttt actacaaccg ctctgtgacc aacgtgaaca caacgatcgt taagaacgtg
    16081 tatgttcaca acgtcaccat caataactac aacattaacc gcgtaagcta taacggcggc
    16141 ccgggaggcg tgcaagcgca tcctctacct gccgaaatgg ttgccatgcg tgagccacat
    16201 ttgcctccgc tccgggtaca gacgcagcac atacaacagg cggcacaaaa ccgcgcgcag
    16261 tttttcaatg taaatcacgg acggccgcag accgtagcac tggagcgccc gctggcaaca
    16321 ccgttccgcg ccccggcggc acagcctcct gtgcgtggcg tggcaaaccc cggcttttct
    16381 gcgcggcagc agcagcacgt cacagctact ccgcagttca atcgcttgga acagcatgcc
    16441 ggacagcaac aggcgcatcg tccggaaatg ctgcaacccg cgccaacgag ccaggttcgg
    16501 ccacaacagc cgcaacagaa caggcccaat ctgatccgtc ccggcacgca tccccaggcc
    16561 caacagcagc agcgcccggc ctctccgcag cgtatcgaag cccgtccgca agagcaggaa
    16621 taccgccctg ctccgcagca gagacaggaa acgagacc
//
LOCUS       DASRBV010000155         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_465240, whole genome shotgun sequence.
ACCESSION   DASRBV010000155 DASRBV010000000
VERSION     DASRBV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465240"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFA02_05940"
     CDS             <1..1200
                     /locus_tag="VFA02_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09427.1"
                     /translation="YTSSAPTLRATAIFEWTGDLAKPGAARLVPVAVWDGNEYQPGGL
                     YLAQPEPLTLETGTLYELEKAGTPQGTFQVNSAENLAGSWIGLGVIRPQTALAKPKPP
                     MSKYPPQVVKEGAQSQQNAEPAKSSAPTPKDDSGRPTLHRHDTADTTPPPVGDDSDRP
                     TLHKRTSATNPSAPVTAAVAPDSGRPKLRYGKPESPEGLVAPSKLEGFPPDLEQMAAV
                     SDVKQSAPHSYAHIWYDPADETKMKAAVEKLAQDALAAENPAAKYITAAGARKPARRK
                     ASAAPPLPVLASEKFNALDLSWSSGVTIVFSAQAAGQNGQTQYITLVAQPDFYGNPQI
                     IFKQITRDDRLDVTPRMKLIDAVDTDGDGRAELLFELRGVSGREFGIYRVMNRSVEQV
                     FHTGPQL"
     gene            1351..2166
                     /locus_tag="VFA02_05945"
     CDS             1351..2166
                     /locus_tag="VFA02_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09428.1"
                     /translation="MKASQGGNMRHALHVSGLAAAAFVCFLTFESAGQTPHSFIAIGS
                     YAQQPPLRAEDAPRFVLSAEMQGDLLMARQRYIAALDAYRQGPSNSPVLWNKMGIANH
                     HMFNLKAAENDYREAIKLDPKYPEAWNNLGAVYYGEKRYHDAEHAYKKAIKLSPKSAT
                     FYSNIGTAYVAEGKYKKGAEAYRTALMLDPNVFNGDPAAKVAEAGPSREMAYLNYLLA
                     KAYAETGRKDEALYYLRKALDEGFSDRKKLLEEKEFAGLRDDPEFRQMMAERR"
     gene            2203..2877
                     /locus_tag="VFA02_05950"
     CDS             2203..2877
                     /locus_tag="VFA02_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZU09429.1"
                     /translation="MKTAISVSKEDYLKAILEAESEGQTVISATLAHWLEVSPPAVSM
                     ALRRLRRDGYVEVKADGIIRLTVKGRETAYRTALRHHLIERMLSEMFGMPWYEVHDEA
                     ERLEHAVSPAFEARLLEKLGEKGTCPHGNSVLPETPAQRARQGVQLLSGAKENAGYTV
                     CSLYERDPKLLRFLHQVGIEPGGMVRVLRKNYDQTVAIETPTGTFTIGAPAADKVWVR
                     KRRAKA"
     gene            2943..5948
                     /locus_tag="VFA02_05955"
     CDS             2943..5948
                     /locus_tag="VFA02_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZU09430.1"
                     /translation="MLTRRHLWIACCLGVFFLAVLRASALDPRYPLRHYGYQAWQTDD
                     GLPQNTVHAVLQTRDGFLWFATEAGLVRFDGAQFAVFDKSNTPQFTSGVINSLLEDKD
                     GRLWISTADGLVEFEKGRFRAFREGDGLPSAMVFSVYQDHNGRLWALTAVGLACYENG
                     RFVNLPEVPAASLVTESPDSTIWFGTGNGVFNDHGGSLFAGEQIQALTAGPDGRVWIG
                     SHQGLKFVITGKAPQDFHLPANLGSDITSLLVASDGHLWIGTANGLAEFDGKTTRVLL
                     SAERISILFEDHEHAIWGATGHGITRIFGGKAETMTSQQGLSSNQALAFYEDREGNLW
                     IGTESGGVGVLRDRKFTTFTSQDGLTDDLVRSVFQARNGIIYAGTNSGGVDQYADGKF
                     MPLSAEKGLSSQVALALADDAQGNLWVGTPDGLDRISKRQTKIFTSADGLADDFVRSL
                     YEDRDGVLWIGTRRGLTRYKNGEFTSWSALDGLGSDLVGAVIQDPDGSHWIGTLAGLT
                     HFTNGHFTNYPMPQAVTALYEDREGTLWIGTNGGSLDARQDGKFVSFPAARSHLPPNI
                     YGILEDANQHLWLATNKGVYRVSKRGLRDLAAGAVAEIHPEAYGTSDGMRISECSSGG
                     HPAAWKAKDGTLWFATLRGVAMVDPAHMPVNRVPPLVAIEQMVVDDSPLSAQPGMKIA
                     PGHTRFAFQYAGLSFAAPQKVRFRYKLDGVDRDWVDPGPRRTAYYTNVPPGEHTFRVM
                     ACNNDGVWSEQAASLTFRLLPRFYQTWWFYLLLLLLVALLVYAVYRWRVRNVESRFNA
                     VLAERNRIAREIHDTLAQGFIAVSVQLEVVARLLNTSVDAAKEHLEQARKLTRDCIQE
                     ARSSIWNLRSQGAGHDDLAAAITSSAERVTANSGTKARIHVGGTRRPLPAAMAAELVR
                     IAQEAMTNAVRHAQAEHIDVTLLFEEKLLRMTIKDDGCGFEGGAEVFRSDGHFGLTGM
                     RERAAQVGAKFEIISKKGCGTEVKVDVPI"
     gene            6006..6632
                     /locus_tag="VFA02_05960"
     CDS             6006..6632
                     /locus_tag="VFA02_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09431.1"
                     /translation="MTEAIRIMVVDDHHIVRQGLVALIRTVPGMLVVAEAADGEQAIE
                     LYRKHKPDVTLMDLRLPKKSGVEAITFIREEFPTARIIVLTTFDGDEDIYRALQAGAK
                     GYLLKGMDTDELTDAIRTVYAGKSRIPAVVAERLAGRMGGPSLTARELEVLKRIVSGR
                     SNKEIAGDLFISEATVKTHINSILSKLGVNDRTQAATTALQRGIVHLD"
     gene            6625..>6918
                     /locus_tag="VFA02_05965"
     CDS             6625..>6918
                     /locus_tag="VFA02_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09432.1"
                     /translation="MTDLNTKHGPGRPTLSRFVCAIVWTVALVPGLFTAVTVVSFAQG
                     TWRAATDAELKALVPVRAPVVQERIETELRTASGITDGKGKYVAGVVLITAGYS"
BASE COUNT         1529 a         1931 c         2021 g         1437 t
ORIGIN      
        1 tacacaagct cggcgccaac gctgcgcgcc accgccattt ttgagtggac aggcgatctg
       61 gcaaaacccg gggcggcgcg tctggttccc gtagcagtct gggacggcaa tgagtatcag
      121 ccgggaggtc tgtatctggc ccagcccgag cccttgacgc tggagaccgg cactctatac
      181 gaactagaga aggccgggac cccgcaggga acatttcagg tcaacagcgc cgagaacctt
      241 gcaggttcat ggattggtct gggtgtgata aggccacaaa cggcattggc caaaccaaag
      301 ccgcctatgt cgaaatatcc cccgcaggtg gtgaaagaag gggcgcagtc gcagcagaat
      361 gcagagccgg cgaagagcag cgcacccact cccaaagatg attccggccg tcccacgctt
      421 catcgtcatg acacggccga taccactccg cctccggtgg gcgatgattc tgaccgcccc
      481 acattacata agcggacatc tgcgaccaat cctagcgcgc ctgtgacagc agcggttgcc
      541 cccgatagtg ggcgaccgaa actccgctat ggcaagcctg agtccccgga agggttagta
      601 gcgccttcca aacttgaggg atttcctcca gacctggaac agatggccgc tgtttctgat
      661 gtaaagcagt ccgcgcccca ttcttacgca catatctggt atgacccggc ggatgagaca
      721 aagatgaagg ctgcggttga aaaactggct caggatgcgc tcgctgcgga aaatccggcg
      781 gcaaaatata taactgcggc aggcgcaaga aagccggcac gacgcaaggc ctctgcggcg
      841 cctcccttgc ctgtcctggc cagtgagaaa ttcaacgcgc ttgatctttc ctggagcagc
      901 ggcgtgacca tcgtcttcag cgcccaggcc gctggtcaaa atggacagac gcaatacatc
      961 acgctggttg cgcagccgga tttttacggc aatccacaga tcatcttcaa acaaatcacc
     1021 cgggatgatc gcctggacgt tactccacgc atgaagctga tagatgctgt ggacacggat
     1081 ggcgacggcc gcgccgagtt gctctttgag ctgcgaggcg tttcgggcag ggaattcggc
     1141 atttaccgcg tcatgaaccg gagtgtcgaa caggtctttc acaccggccc gcagttgtaa
     1201 cgcaaggacc gcgccagatg accattttgg cgctttttat ccagtcaaca ccgcttttat
     1261 cgcttgttct ttatctaaag agttacaatt aggggccaaa ggctgggtct gtccggacac
     1321 agctcatccc ccgcgcccgg cagatgcagg atgaaagcct cgcaaggagg caatatgcgc
     1381 catgctctcc acgtctcagg tcttgctgcc gcagcgtttg tttgttttct cacgtttgag
     1441 tccgcaggac agacacccca ttcgttcatt gcgataggca gttacgccca acaacctccg
     1501 ctcagagcag aagacgcgcc ccgtttcgtc ctgagcgcgg aaatgcaggg cgacctgctg
     1561 atggcgcgcc agagatacat tgctgcgctt gacgcctacc gccagggtcc atcgaattca
     1621 ccggtactct ggaacaagat gggtattgcc aaccatcaca tgttcaatct caaggccgct
     1681 gagaacgatt accgggaagc gatcaagctc gatccaaaat atccggaggc atggaacaat
     1741 ctcggcgcgg tctattatgg cgagaagcgg tatcatgatg ccgagcacgc ctataaaaag
     1801 gccattaagc tttccccgaa gtccgctacc ttctatagca atatagggac agcttatgtg
     1861 gccgaaggca aatataagaa aggcgcggag gcctatcgaa cagcactcat gcttgatccc
     1921 aatgtcttta atggagatcc cgccgccaaa gttgctgagg ctgggccatc acgagagatg
     1981 gcttatctga actatcttct tgcaaaggcc tatgctgaaa ccggaagaaa agacgaggca
     2041 ctttattatc tgcgtaaagc gctcgacgag gggttcagcg accgcaagaa gctactggaa
     2101 gaaaaggaat ttgcgggact gcgcgacgat cctgagttca ggcaaatgat ggccgaacgg
     2161 cgctagggcc tcatagcagc cagtagttgt atcctgattg cggtgaagac tgcaatcagc
     2221 gtttccaaag aggactatct caaggccatc cttgaagctg aaagcgaagg tcagacggtc
     2281 atttccgcga cgctggccca ttggcttgag gtttctcccc cggcggtctc aatggcgctc
     2341 cgccgcctca ggcgcgatgg atatgtcgag gtcaaagccg atggcatcat ccgtcttacg
     2401 gtaaagggca gagaaaccgc ctatcgcacc gcactgcgcc atcacctgat agagcgcatg
     2461 ttatctgaga tgttcggaat gccctggtat gaagtgcatg atgaagccga acgcctggag
     2521 cacgccgtct cacccgcttt tgaagcccgt ctccttgaaa aattggggga aaaaggtacc
     2581 tgtccccatg gaaattctgt tctgccggaa acaccggcgc agcgcgcaag gcagggagtg
     2641 cagttgcttt cgggagcaaa agagaacgcc ggctacaccg tctgcagcct gtatgagcgt
     2701 gatccgaagc tgctgcgctt tctgcaccag gtgggcattg agccgggcgg aatggtgcgt
     2761 gtcctccgga aaaattatga ccagacggtg gccattgaga cgccgacagg cacgtttacc
     2821 atcggcgctc cggctgccga caaggtctgg gtaaggaagc gacgggcaaa ggcctgatct
     2881 ccaccttttg gttgagtgcc ggccactact ctggatgtgt actattcctg taagtcccgc
     2941 tcatgctgac acgccgccat ctttggatcg cctgctgcct gggtgtgttc tttttggccg
     3001 tactccgggc gtccgcgctc gacccgcgct acccgctgcg gcactatggt tatcaagcat
     3061 ggcagaccga cgacgggctt ccacaaaaca ccgtccatgc tgtgttgcaa acccgcgacg
     3121 gctttctctg gtttgcgacc gaggctgggc tggtccgctt tgatggcgct caatttgctg
     3181 tcttcgataa gtcgaacacg ccacagttca caagcggagt catcaacagc ctgctggaag
     3241 ataaagacgg aaggctctgg atcagcacgg cggacggcct ggtcgagttt gaaaagggtc
     3301 gtttccgcgc ttttcgtgaa ggtgatggac tcccctcggc aatggtcttc tccgtctatc
     3361 aggaccataa cggaagattg tgggccctca ccgctgtggg tttggcatgc tacgagaatg
     3421 gccgttttgt aaacctgcct gaagtgccgg cggcaagcct tgtgaccgaa tcccctgaca
     3481 gcaccatctg gttcggaaca ggaaacggcg tattcaatga tcatggaggc agcctttttg
     3541 caggagagca aattcaggcg ctgactgccg gccctgatgg ccgcgtctgg attggttcac
     3601 accaggggct gaaatttgta atcactggca aggccccgca ggactttcac ctccctgcaa
     3661 acctgggcag cgacatcacc agcctccttg tcgctagcga tggacatctc tggatcggca
     3721 ctgccaatgg tctggcagaa tttgacggca agaccactcg cgttcttctg tctgcggaac
     3781 ggatctcaat attgtttgaa gatcacgagc acgccatctg gggcgcaacc ggacacggca
     3841 ttacccgcat ctttggcgga aaagcggaaa caatgacctc gcagcaaggg ctttccagca
     3901 accaggcgct tgctttttat gaagaccgcg aaggcaatct ctggattggc accgagtctg
     3961 gcggtgtagg cgttctgcgc gaccgcaagt tcaccacatt tacttcgcag gacgggctga
     4021 ccgacgatct tgtgcgctct gtctttcagg cgcgaaacgg cataatctat gcaggaacaa
     4081 acagtggcgg cgtggaccaa tatgccgatg gaaaattcat gccgctttct gccgaaaagg
     4141 gtctgtcgag ccaggtagca ctggcgctag ccgatgatgc gcaggggaac ctctgggtgg
     4201 gtacgccgga tgggctggac cgcatcagta agcgccagac gaaaatcttt acctctgccg
     4261 acggtcttgc cgatgatttt gttcgctctc tttacgaaga tcgcgatggc gtactgtgga
     4321 ttggcacccg gcgcggattg acgcgctata aaaatggcga gtttacatcc tggtcggccc
     4381 tggacggtct gggtagcgac ttggtgggcg ctgtgattca ggaccccgac ggcagtcact
     4441 ggattggtac gctcgctggc ctcactcact ttaccaatgg ccatttcacc aactatccca
     4501 tgccgcaggc cgtgactgcc ttgtatgaag accgcgaggg cactctctgg attggcacca
     4561 atggcgggag tctcgacgca cggcaggatg gaaaatttgt cagcttcccg gctgcgcgtt
     4621 cgcacctccc accaaatatc tacggcattc tggaagatgc aaaccagcat ctttggctgg
     4681 ccacaaacaa aggcgtatat cgcgtgagca aacgcggtct gcgcgatctg gctgctggtg
     4741 cggtcgcaga gattcatccg gaagcctatg ggaccagcga cggaatgcgc atcagcgaat
     4801 gcagcagcgg cggacatcct gccgcgtgga aagcaaaaga cggaacgctg tggtttgcaa
     4861 cgctgcgcgg tgttgccatg gtagatccgg cccatatgcc ggtgaaccgg gtgcctccgc
     4921 tggttgcgat tgagcagatg gtggtggatg actcgcctct gtccgcgcag ccgggaatga
     4981 agatcgctcc cggacatacg cgctttgcct ttcaatatgc cggactcagc tttgcagcgc
     5041 cgcagaaggt ccgcttccgc tacaagctcg atggtgtgga ccgcgactgg gtagatccgg
     5101 gcccgcgccg cacagcctac tacacgaatg ttcctccggg cgagcacaca tttcgtgtga
     5161 tggcctgtaa caacgacggc gtctggagcg agcaggccgc gtcactcacc tttcgactgc
     5221 ttccgcgctt ttatcagacg tggtggtttt acctgctgtt gctgcttctg gtcgcattgc
     5281 ttgtatatgc ggtctaccgc tggcgtgtgc gcaatgttga atcgcgcttc aacgctgtgc
     5341 tcgcggaacg gaatcgcatc gcgcgcgaga ttcacgacac attggcccag ggattcatcg
     5401 ccgtttctgt gcagctggaa gtcgtggcgc gcctgctgaa cacatctgtg gacgcagcaa
     5461 aagagcacct ggaacaggcg cgcaagttga cgcgagattg cattcaggag gcccgcagct
     5521 ccatctggaa cctgcgttca cagggcgcag gtcatgatga tctggctgct gccattacca
     5581 gctctgccga acgggtgact gcaaacagcg gtacaaaggc gcgtattcat gtgggcggaa
     5641 cgcgccggcc acttcctgcc gcaatggcgg cggagctggt ccgcatcgcg caggaggcga
     5701 tgaccaacgc ggtgcgccat gcgcaggcgg agcacataga tgtgactttg ctctttgaag
     5761 aaaagctgct ccgcatgaca atcaaagacg acggctgtgg ctttgaaggc ggtgcggaag
     5821 tcttccggag cgatgggcat tttggattga caggaatgcg cgagcgggcg gcgcaggttg
     5881 gagcaaaatt tgaaatcatc agcaaaaaag gatgtggtac agaggtgaag gtggatgtgc
     5941 cgatttaaac gaccagcaga catctctttc ccctctggat ggaagcaccc attcaggaaa
     6001 gttctatgac agaagcgatt cgcattatgg tcgtggatga ccatcacatt gtgaggcagg
     6061 gactggtggc actcattcgc accgttcctg gaatgctcgt cgtggctgaa gctgcggacg
     6121 gcgaacaggc catcgagttg tatcgcaaac acaaaccgga tgtcacgctc atggaccttc
     6181 gtctgccgaa gaagagcggc gtcgaggcca ttacattcat ccgcgaagag ttcccaacgg
     6241 cccgcatcat cgtccttacg accttcgacg gcgatgaaga catctatcgg gccctgcagg
     6301 caggtgcaaa gggctatctt ctcaaaggaa tggacaccga cgagttgacc gacgcgattc
     6361 gtaccgtata tgcaggaaaa tcgcgcattc ccgccgttgt tgccgagcgc ctcgccggac
     6421 gtatgggcgg cccttcgctg acggcgcgcg agctggaagt cctgaagcgc attgtgagtg
     6481 gccgcagtaa taaggaaatc gccggagacc tgttcatctc cgaggcgacg gtaaagaccc
     6541 acatcaacag cattctcagc aagctgggag tgaatgacag aacgcaggcg gcaacaaccg
     6601 ccctgcagcg ggggattgtg catcttgact gacctgaata caaaacacgg ccccggccgc
     6661 cccactctgt cgcgcttcgt ttgcgcgatc gtgtggacgg ttgctcttgt tccgggcttg
     6721 ttcacggccg taacagttgt ttcatttgcg cagggaacat ggcgcgctgc caccgatgca
     6781 gagctgaagg cactggttcc cgtccgcgct ccggttgtgc aggagcgcat tgaaaccgag
     6841 cttcgcactg catccggtat cacagacggt aaaggcaaat acgtcgccgg ggtggtgctg
     6901 attacggccg gttattca
//
LOCUS       DASRBV010000156         6371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_567411, whole genome shotgun sequence.
ACCESSION   DASRBV010000156 DASRBV010000000
VERSION     DASRBV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567411"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFA02_05970"
     CDS             complement(<1..473)
                     /locus_tag="VFA02_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09433.1"
                     /translation="MKNIALRFSFPHGLKTWLASVAFCAMCAGHVCAQSGAAQDAGAK
                     KAHDLLNAMIQAMGGDRWLNLQNTYIEGRTSGFYQGKPTGMIGDFYWWHVFPSRDRID
                     LSKKHDVVDIFDGKQGWEITYRGKRPLPQDQLDDFIRRRDHSIETVVKVWMKDPNT"
     gene            complement(491..1255)
                     /locus_tag="VFA02_05975"
     CDS             complement(491..1255)
                     /locus_tag="VFA02_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU09434.1"
                     /translation="MSAVSLSLAGKVALITGGSRGIGAECVRLFHEAGAKIAFNYRAA
                     RNQAESLAASCGAVAIEQELSSPEDGRSLVAKTIAAFGQIDCLVVNHGVWPPEEVSIA
                     KMSQAQWRRTLAVNLDSVFGLVQAAVAQMKKQHTGGHIVLVSSTAGQRGEAFHADYAA
                     TKGAIISLTKSLSSELAREGIYVNCVAPGWVATDMSAPALNDPVTREKVFAAIPLGRV
                     ATPREIAGPILFLCTPFAGFISGEIVNVNGGAVLVG"
     gene            complement(1300..2310)
                     /gene="era"
                     /locus_tag="VFA02_05980"
     CDS             complement(1300..2310)
                     /gene="era"
                     /locus_tag="VFA02_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZU09435.1"
                     /translation="MKPWRKQAAQPRRGNRPLRSGFVSILGRPNVGKSTLLNQLVGEK
                     VAIVTSKPQTTRTRIHGIVEVPAKKGKHPAAQIVFVDTPGVHRPDSWLDKRMMQEVHD
                     ALESRDIVLLMVDATRRQEFSAPESEDSFTLSLVRKLDCPVFLLINKIDLLPKDKLLP
                     MIAELSALHSFAEVIPVSARKGDGLDRLLVKLVEYLPEGERYFPKDQFTDQPLRFMVA
                     ELIRERILQETGEEVPYASAVVVERFEEPGPGAKNPVTRISAAIYCERSGQKAILIGK
                     GGSKLKSIGMNARREIESLLGTRVFLELRVIVEEGWRERRGFVESLDWRKQIEELGKR
                     EQ"
     gene            complement(2264..2884)
                     /locus_tag="VFA02_05985"
     CDS             complement(2264..2884)
                     /locus_tag="VFA02_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU09436.1"
                     /translation="MLRSFLTIVLAAVVMAPAAHPQSSPQTALKPPAGARVAIIEFAD
                     LECPSCAHENPVLKDAAAKYRVPWVRHDFPIQYHRWSTQAAINARWFDEKSKKLGDDY
                     RDAVFANQINIATPDDLRSFTEKFAKDHGMALPFIMDPQGKLAAAVKADYDLGVRLGV
                     HQTPTVWIVTDNHGSINARQVNDFNKLYSMLDEAVAETGSATQARK"
     gene            2917..3318
                     /locus_tag="VFA02_05990"
     CDS             2917..3318
                     /locus_tag="VFA02_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZU09437.1"
                     /translation="MRYIIAFFAIAGIVISILALRVHYSTDVQPCDINAHWDCGIVNH
                     SRYAVVHGFPVAAIGIAGYAIIAILALTERHKLLLVAAIAGLAYSLYLTSIEARILEV
                     WCLYCVISQTIIAIITLLAIIAALRPGRARA"
     gene            complement(3281..4597)
                     /locus_tag="VFA02_05995"
     CDS             complement(3281..4597)
                     /locus_tag="VFA02_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZU09438.1"
                     /translation="MTIAAAVLIACLLVVLTLASYADRIYSEMGKFLAREFQENLDAW
                     EELIEPRIGLSRDHLAISAAVLSQLSLACLTLIFGMLLFQRNSPADRVTFPEVAQAVL
                     GVVLVIVLFNRLLPYVLFVRTRGAWVAHFRWLFRLLFYLVLPVTFFMGFLSSIAALAE
                     APRHEEQPGASEAVEALIEAGEEEGIIEESDRELVRSAVEFGDKVVREVMTPRPEMIA
                     ISGKATLEELLQLIDKHPVSRVPVYDGSLDQITGIAFAHDLLRVTDEAARTRSVASIQ
                     RPAAFVPETKKVNELLREMQREKQHMRIVIDEYGGVAGLVTIEDLLEEIVGSIADEHE
                     TDELETPRRESDGSWLVPGSLEVPRIEDLFGDNWQMPEGYQATTVGGLVSEIAGRIPM
                     PGEVVEEDGLRFEVLSSTDRLIERLRISPRQDGLPTPGHGLDATRQ"
     gene            complement(4594..5073)
                     /gene="ybeY"
                     /locus_tag="VFA02_06000"
     CDS             complement(4594..5073)
                     /gene="ybeY"
                     /locus_tag="VFA02_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZU09439.1"
                     /translation="MILIEPDADTSQNRNALKMRDLRSFLRKAASAARLQGEVSVLLA
                     TDASIRTLNRDFRNKNKPTDVLSFPADADHPNPKVAGDLAISLDTARKQAEEHGHPLQ
                     VELKILLLHGVLHLAGYDHESDTGQMARRESALRKQLGLPVGLIQRSNAKKSRGRVP"
     gene            complement(5123..6136)
                     /locus_tag="VFA02_06005"
     CDS             complement(5123..6136)
                     /locus_tag="VFA02_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZU09440.1"
                     /translation="MMKKALEITPDLGPLFGTRDENLRLMEDGLNVRIDLKSDAVQVE
                     GPQDGLDRVERIFSDYEYLRRNGVNLHNGELNGMLRLVVADPSVTLRGLVESGKQRSA
                     GIKRMVQPRSLNQRHYVEAIEQADMVFGIGPAGTGKTYLAVAMAAAALLAKKVSRIVL
                     VRPAVEAGERLGFLPGSLQEKVDPYLRPLYDALYDLLEPERVDKMLERNVIEVAPLAF
                     MRGRTLNDAFIIMDEAQNTTSEQMKMFLTRLGNNSKAVITGDVTQIDLPNPRKSGLIE
                     AMNVLEGVDGIRFIHFEDGDVVRHHLVQRIIRAYDSFGRAQQELPLGIPETDDTRTSL
                     KPQ"
BASE COUNT         1311 a         1769 c         1896 g         1395 t
ORIGIN      
        1 gtattcgggt ctttcatcca gaccttcacg acggtctcaa tggaatggtc gcggcggcgg
       61 atgaagtcgt ccaactggtc ctgcgggagg gggcgcttgc cgcggtatgt aatctcccaa
      121 ccttgtttgc cgtcaaaaat atcaaccacg tcgtgttttt tgctgaggtc aatgcggtcc
      181 cggctgggga agacatgcca ccagtaaaaa tcgccaatca tcccggtcgg tttgccctgg
      241 tagaagcctg aagtccggcc ctcgatatag gtattttgaa gattgagcca gcggtcaccg
      301 cccatcgcct gaatcatcgc attcagaaga tcatgggcct tcttcgcgcc agcgtcctgg
      361 gccgcgcctg attgcgcgca tacatggcct gcgcacattg cgcaaaaggc aacggaagca
      421 agccatgtct taagcccatg cggaaaagaa aacctcaatg caatgttttt catgaatgct
      481 ttcaacctca tcagcccacc aggacggccc cgccgtttac attgacgatt tcgccgctga
      541 taaagcctgc aaatggcgtg cagaggaaca gaatcggccc tgcaatctcg cgcggagtgg
      601 cgacgcgccc gagcggaatg gcggcgaaga ccttctcccg ggtcacggga tcgttcaatg
      661 ccggagcgga catatcggtg gccacccatc ctggagcgac gcagtttaca taaatgcctt
      721 cgcgggccag ttcgctggag agacttttgg taagactgat gatggcgcct ttggttgcgg
      781 cgtagtcggc gtggaaggcc tctccgcgct gtcctgccgt ggagctgacg agaacaatgt
      841 gtccgcctgt gtgctgtttt ttcatctgcg ccacagccgc ctgcaccagg ccgaaaacgc
      901 tgtcgagatt gacggcgagc gttctgcgcc actgcgcctg gctcatcttt gcgatggaaa
      961 cctcctccgg tggccagacc ccatgattta ccacgaggca atctatctga ccgaaagccg
     1021 cgatggtctt tgcaaccaaa gagcgcccat cttcaggaga agaaagctcc tgctcgatgg
     1081 caacggcgcc acatgaagcg gccagcgatt ctgcctgatt tctggcagca cggtaattga
     1141 atgcaatctt tgcgccggcc tcatgaaaca ggcgcacgca ttcagcgccg attccgcgcg
     1201 acccgccggt aatgagggcc acctttcctg caagtgacaa cgaaactgcg ctcatgtttc
     1261 gacgctaaat ggaatccgcc atccaggcaa tccggcaagt tactgttcgc gtttgccgag
     1321 ttcctcaatc tgtttgcgcc agtcaagcga ttcgacaaag ccccgacgtt cgcgccagcc
     1381 ttcttcgacg atgacgcgga gttccagaaa gacgcgcgtt ccgagcagtg actcaatctc
     1441 cctgcgggca ttcatgccga tacttttgag cttgcttccg cccttgccga tgaggatggc
     1501 cttctgtccg gaacgctcgc agtaaatggc ggcagagatg cgcgtgaccg gattttttgc
     1561 gcctggcccc ggttcttcaa atcgctctac gaccacggcg gaggcgtagg ggacttcttc
     1621 tccggtctcc tgcaggatcc gctcgcgaat caattctgcc accataaagc gcagaggctg
     1681 atccgtgaat tggtctttgg gaaaataccg ctcgccttcc ggcagatact cgaccagttt
     1741 taccagcagg cggtccagac catcgccttt gcgcgcagaa accggaatga cctcggcaaa
     1801 ggagtgcagc gcgctgagtt ctgcaatcat cggcagcagc ttgtcttttg gaaggaggtc
     1861 aattttgttg atgaggagga agacagggca gtcgagtttg cggacgaggc tgagagtgaa
     1921 tgaatcttcg ctttcggggg cggaaaattc ctgccgacgg gtggcgtcta ccatgagcag
     1981 aacaatgtca cggctttcca gcgcgtcgtg tacctcctgc atcatgcgct tgtcgagcca
     2041 tgaatcgggt ctgtggacgc cgggagtgtc aacaaagacg atctgtgctg ccgggtgctt
     2101 gccttttttt gccgggactt cgacgatgcc gtgaatgcgc gtgcgcgtcg tctgcggctt
     2161 gctggtgaca atggcgacct tttcgccgac gagttgattg agcagtgtgg atttaccgac
     2221 attgggccgg ccaagaatcg agacaaaacc tgagcgcaag ggcctatttc ctcgcctggg
     2281 ttgcgctgcc tgtttccgcc acggcttcat ccaacatgga gtaaagcttg ttgaagtcgt
     2341 tgacttgccg ggcgttgatg gagccgtggt tatcggtcac aatccaaact gtgggtgtct
     2401 ggtggacgcc aagccggaca ccgagatcat agtcggcctt gactgccgcg gcgagtttgc
     2461 cttgcgggtc catgataaag ggcagggcca tgccgtggtc tttggcgaac ttttctgtaa
     2521 aggagcgcag atcatccggc gtagcgatat tgatctggtt ggcaaagaca gcgtcgcggt
     2581 aatcgtcgcc gagcttcttt gacttttcat cgaaccagcg cgcgttgatg gctgcctggg
     2641 tgctccagcg atggtattgg atggggaaat cgtgccgcac ccaggggacg cgatatttgg
     2701 cggcggcgtc tttcaatacc ggattttcgt gcgcgcagct cggacactcg agatcggcaa
     2761 attcgataat tgccactctt gcgcccgccg gaggcttcag tgctgtctgc gggctggatt
     2821 gcgggtgggc tgccggcgcc atgacaaccg cagccagaac aatggtcaga aaagaacgca
     2881 gcatatctct catttcattc tattagactg gttgcaatgc gttacatcat tgcatttttt
     2941 gccattgcgg gaatcgtcat ttccatcctg gcgctgcgcg tccactactc gaccgacgta
     3001 cagccgtgcg acatcaatgc tcactgggac tgcggcattg tgaaccatag ccgctatgcg
     3061 gtggtgcatg gttttccggt agcggcgatc ggcattgccg gatatgcaat catcgcgatt
     3121 cttgcgctga ccgagcgcca taaactgctg ctggttgcgg cgattgcggg gctggcgtac
     3181 tcgctttacc ttaccagcat tgaggcgcga attctggagg tctggtgcct gtattgcgtg
     3241 atttcgcaga cgatcatcgc aatcattaca ctgctggcca ttattgccgc gttgcgtcca
     3301 ggccgtgccc gggcgtaggc aggccgtcct gccgggggga gatacgcagg cgctcaataa
     3361 gacggtctgt ggatgagagc acctcgaagc gcaaaccatc ttcttccaca acttctcccg
     3421 gcataggaat acggcctgca atttcactga cgaggccgcc gactgtcgtg gcctgatagc
     3481 cttcgggcat ctgccagttg tcgccaaaca ggtcttcaat gcgggggact tccaggcttc
     3541 cgggaacgag ccaggagcca tcggattcgc gcctgggggt ctccagctcg tcggtttcgt
     3601 gttcgtcggc aatggagccg acgatttctt cgagcaggtc ttcaatcgtg accaggccgg
     3661 cgacgccgcc gtattcatca atcacaatgc gcatgtgctg cttttcacgc tgcatttcgc
     3721 gcagaagttc gttcactttc tttgtttcag gaacgaaggc agcagggcgc tgaatgctgg
     3781 caacggagcg agtccgcgca gcttcatcgg ttacgcgcag caggtcgtgc gcaaaggcga
     3841 tgccggtgat ctggtcaagc gagccatcat aaacaggcac gcgcgaaacc ggatgcttgt
     3901 cgatcaattg cagcaattct tcaagggtgg ccttgccgga gatggcaatc atctccgggc
     3961 ggggcgtcat gacctcgcgc acgactttat cgccgaactc gacggcagaa cgcaccagct
     4021 cgcggtcgct ttcctcgata atgccttctt cttcgcctgc ttcaatgagc gcttctacgg
     4081 cttctgacgc gccgggttgt tcttcgtgcc tgggcgcctc agccagggca gcaatcgagg
     4141 agaggaaccc catgaaaaag gtgacaggca acacgagata aaacagcagg cggaaaagcc
     4201 agcggaagtg cgccacccag gcgccgcgtg tgcgcacgaa cagaacgtag ggcagcaggc
     4261 gattgaaaag cacaatcacg agcactacgc caagcacggc ctgagcaact tcaggaaagg
     4321 tgacgcggtc ggccggcgaa ttccgctgaa acaggagcat tccgaagatg agcgtgaggc
     4381 aggcaagcga aagctgcgaa agcactgccg cagagatggc cagatggtcg cggctcaacc
     4441 cgatccgcgg ctcgatcagc tcttcccatg cgtcgaggtt ctcctggaac tcgcgcgcaa
     4501 gaaatttgcc catctcggaa taaatgcggt ctgcataaga ggccagcgtg agcaccacca
     4561 gcagacatgc aatcaggact gcggctgcaa tggtcatggc acacgccctc tggacttctt
     4621 tgcattgctc ctctggatca gtcctacggg caggccaagc tgtttgcgca aagcgctttc
     4681 cctgcgggcc atctgtccgg tgtccgattc gtgatcataa ccggcaaggt gcaggacgcc
     4741 atgcaggagc aggattttca actcgacctg caggggatgg ccgtgttctt cggcctgctt
     4801 tcgggccgta tccagcgaga tggccagatc gcctgcaact ttggggttgg ggtggtctgc
     4861 atctgccggg aaagagagca cgtccgtggg cttgttcttg ttgcggaagt cgcggttcag
     4921 agtgcggatg gaggcatcgg ttgccagcag aacggagacc tctccctgca accgggcggc
     4981 gctggcggct tttcgcagaa aactgcgaag atcgcgcatc ttgagcgcgt tccggttctg
     5041 ggaagtatcg gcgtcaggct cgatgaggat catgcagaat gaggaggact ggaacagccc
     5101 tcctcaagga aattatcgca aattactgcg gcttcagtga tgtacgtgta tcgtctgttt
     5161 cgggaatccc aagtggaagc tcctgctggg cgcgaccgaa gctgtcataa gcgcggataa
     5221 tgcgctgcac cagatgatgg cgcacgacat cgccatcttc aaagtgaatg aagcgaatgc
     5281 catctacgcc ctcgagcacg ttcatggctt caatcagacc ggacttgcgc gggttcggca
     5341 gatcaatctg ggtgacgtcg ccggtaatga cggccttcga gttgttgccg aggcgcgtca
     5401 gaaacatctt catctgctcg ctggtggtgt tctgggcctc gtccatgatg atgaaggcgt
     5461 cattgagcgt gcggccgcgc ataaaggcaa ggggcgctac ttcgatgacg ttgcgctcca
     5521 gcattttgtc cacgcgctcc ggctccagaa ggtcgtagag tgcgtcatat aacgggcgca
     5581 gataggggtc taccttctcc tgtagactgc cgggcaggaa gccgagacgt tcaccggcct
     5641 cgacggcagg gcgcaccagc acgatgcggc tgactttttt ggcaagcagt gcggcggcgg
     5701 ccatcgccac cgcaagatag gttttacctg tgcctgccgg gccgatgccg aagaccatgt
     5761 ccgcctgctc gatggcttca acatagtgac gctggttcag cgagcggggc tggaccatgc
     5821 gcttgattcc ggcagagcgc tgctttccgc tttccacaag gccgcgcagt gtgaccgagg
     5881 ggtcggccac aacaaggcgc agcatgccat tcagttcgcc attgtgcaga ttgacaccat
     5941 tgcggcgcag gtactcgtag tcggaaaaga tgcgctccac gcggtcgagg ccgtcctgcg
     6001 ggccttctac ctggacggcg tccgatttca ggtcaatacg cacgttcagg ccatcttcca
     6061 taagtcttag attttcatcg cgggtcccga agagaggccc aagatcaggc gtaatttcga
     6121 gcgctttttt catcaagcgg agagacaacc tcctgtggcc ggcaaagcgg tcacgatgac
     6181 tgaagtggat tgtggggctt gcgaagcaga gttcaagcgg aaaggcacca cattttttag
     6241 atgcggctgc cggagcttcg gattggcgga atgcaccaaa attcggctcg tattgcgctc
     6301 agagtagccc cgctctggtt tcacgtcaat agggggcaga taaaattttt gtaagagtgt
     6361 atcaaaacag a
//
LOCUS       DASRBV010000157         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_109282, whole genome shotgun sequence.
ACCESSION   DASRBV010000157 DASRBV010000000
VERSION     DASRBV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109282"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..720)
                     /locus_tag="VFA02_06010"
     CDS             complement(241..720)
                     /locus_tag="VFA02_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZU09441.1"
                     /translation="MYTLVRKTLVALFAVILFAGLCGHAQARNIYSDTADAHKDIQQA
                     LELAKREHKRVILDFGGNWCGDCQVLDIYFHQQPNLDLLNKYFVLVHINVGRFDKNTD
                     LAEKYQVPLKKGVPALAVLSASGKLLYSQKNGEFEAMRRMDAASVTQFLNEWKPAKV"
     gene            complement(775..1254)
                     /locus_tag="VFA02_06015"
     CDS             complement(775..1254)
                     /locus_tag="VFA02_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09442.1"
                     /translation="MTLLDAPAYDARRARRNRNIVVSIVIVIVVIGLTGVLGFVTGHG
                     WFFRTIPAEHRVNKFFSALESQDYKKAYALWTHDQNWDQHPDQYKPYDYSQFYKDWGP
                     SGDYGVIKGHSIVISKAVGNGVVMGVDINGGKTPIFLRVDDQSKQIGFSPVELYVGP"
     gene            complement(1310..2491)
                     /locus_tag="VFA02_06020"
     CDS             complement(1310..2491)
                     /locus_tag="VFA02_06020"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZU09443.1"
                     /translation="MSKLRVGVLFGGRSGEHEVSLLSAASVLKAIDRKKYDVIPIGIT
                     KEGRWVTATDAERLLAGEPVPGRHFRAGDPEATAPAAVLAKGEAVIVPPVPSESKTGS
                     LVPLETERHPAHQALSVDVIFPVLHGTFGEDGTIQGLFELAGIAYVGSGVLGSATGMD
                     KDVMKRLFASAKLPITKHVTFLRGEWESSRKKITARIEAALRYPVFVKPANLGSSVGI
                     SKAHDRKELGPAIEMAANYDRKIVVEQGVGGKRGKARELEVAVLGNDSPKASVVGEVV
                     PVKEFYDYEAKYLSEGSDLIIPAKLTKQQTKQVQQMAVAAFKVCDCSGLARVDFLMEP
                     GKSGRIYLNEINTLPGFTAISMYPKLWAASGVPYPELIDRLIALALERRAEKDRTQYS
                     R"
     gene            complement(2642..3610)
                     /gene="rocF"
                     /locus_tag="VFA02_06025"
     CDS             complement(2642..3610)
                     /gene="rocF"
                     /locus_tag="VFA02_06025"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZU09444.1"
                     /translation="MSKIGETLAASHSGVQTLPPREICIIGVPLDLGASRRGVDMGPS
                     AVRVAGLEARLEALGHNVTDGGNIGVAIAETRHVGEESARYLKEITETCTRAAEHVIQ
                     ALEEGKTPVIIGGDHSVAAGSMSGVAEFYRRRNQKVGLIWIDAHSDLNTPETSPSGNV
                     HGMPLAALLGLGPDSLANICGFSPKVLPENTVLIGVRDIDATEKENIRRAGLTEVYTM
                     RDIDERGMRAVMEEALRAAGRGTAGYHVSLDMDWVDPEDAPGVGTPVRGGATYREAHL
                     AMEIIADHGRMLSLEVVEVNPVIDEHNRTADLAVELISSAFGKKIL"
     gene            complement(3687..5042)
                     /gene="asnS"
                     /locus_tag="VFA02_06030"
     CDS             complement(3687..5042)
                     /gene="asnS"
                     /locus_tag="VFA02_06030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZU09445.1"
                     /translation="MSEPTTVAATTEAPVSTIARVGEHEGKSVTLKGWLYNLRESGKL
                     LFPIFRDGTGTIQGVVPKAAVTPEVFETVKGLTQESSVIVTGKVRADKRAPGGYELDV
                     ENVEAVQRVSEETPFPISLKEHGTDFLMEHRHLWIRTPRQSAILRIRAEIIKAARDFL
                     DDNGFVLTDSPVLTPAACEGTSTLFPVEYFGDEAFLTQSGQLYIESTAMALGKVYSFG
                     PTFRAEKSKTRRHLTEFWMVEPEWAYATLDDLMGLAEQFISFIVDRVLTRRAADLKVI
                     GRDTNKLEAIKAPFPRISYDEAAQMLKDAHAKGLLEQPFEYGNDFGSPDETYISSQFD
                     RPVMVHRYPAEVKAFYMEPDPQDPKYALCVDVLAPEGYGEIIGGSQRIASFDLLKKRI
                     EEHNLPMDAFQWYLDLRRYGSVPHSGFGMGIERAVAWICGLEHVRETIPFARTLNRIY
                     P"
     gene            complement(5081..6628)
                     /locus_tag="VFA02_06035"
     CDS             complement(5081..6628)
                     /locus_tag="VFA02_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09446.1"
                     /translation="MRSVMLLFVHVAAFFACGMAQTVTYPLTIDDASIENAYILHAKN
                     WPSKFGLPNPVIIRSNAGERWYQIETSRGNFSPTFGNLFTPETGWVPVAEKNHTQLIY
                     TFNTVPAWAAGEPGPGPAKRAPLDIDQKNETCKAPLDGVVSPDGDCIWKEWVTALMQK
                     NCGVSSAPSSPLTGRCHIHYFEAWNEFNAPIFWDDSLSHLAKMANDMAIIVRIYCGDC
                     KVIGGSTSAGGVGRSGDGPAGSGEFDKALGEFLDAWHHIPNASLPDIVSFHAYPSRTN
                     VFPVPMPETNVSEGDAKCAQFSVPNVSCQYAIVDQPGRVRAVLAKRQSFLSPSTPVWD
                     TESGWYGNKNLFHGVDANGYADEITGRMRQAFLARETILLANEGVGVNLWYEADHQCN
                     GTLIGFGYPQSSPEMHQCPNDPVIPPGLTPAGRALVVVHGWLHGGVFNGPCRPQGGVW
                     SCPITTQQGQRGLIAWTAKIGATATVTPPQGGFSYAHNLDGQTQRLTPGTPLPIEMRP
                     TLFDNAP"
     gene            complement(6673..>7016)
                     /locus_tag="VFA02_06040"
     CDS             complement(6673..>7016)
                     /locus_tag="VFA02_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZU09447.1"
                     /translation="LPREAPQQFFIREGERAWMVRPADIRLLESEGNYTRICFGEHRP
                     LVLRSLQALHERLGSQHFFRANRNQVINLRWVESMELEPDLSLTAVLKDGTRVGLSRR
                     QSQLFREQMAL"
BASE COUNT         1493 a         2068 c         1903 g         1552 t
ORIGIN      
        1 gccggaaaga gactcagaat acctgaatac gccgcagctt tggcaatgcc aaaggcgccg
       61 tggttgaagg cactccacgc cgcccgccgg agatgtgtcc agagctgccg caacatacgt
      121 gtcgagggta actgaagtga aaggaaggtg gaagccgatt ttattttttg cacaaggggt
      181 ctggatcgta agagaaggat ccagacccaa gatcctcatc ctgcggaacc cggaatacgc
      241 ctaaaccttc gcgggcttcc actcattcag aaactgcgtg actgaagcag catccatccg
      301 gcgcatggcc tcgaattcac cgtttttctg gctgtagagc agtttgccgc tggcgctgag
      361 aacggccagc gcaggcaccc cctttttcag cggaacctgg tacttttcag caagatcggt
      421 gttcttgtcg aatcggccca cgttaatgtg gacaagaaca aaatacttgt tcaacaggtc
      481 caggttcggt tgctgatgaa agtagatatc gagtacctgg cagtcaccgc accagtttcc
      541 gccaaaatca agaattacac gcttgtgctc gcgctttgca agttcgagtg cctgctggat
      601 atctttgtgc gcgtcggcag tatcggaata aatattgcgc gcctgcgcgt gaccacacaa
      661 accggcaaac agaatgacgg caaataaagc gacgagagtt ttacggacaa gtgtgtacat
      721 ggttgtagtt aggatgccac agaatgctgg ctggctcctg aatttcaccc ggatctatgg
      781 tccaacgtaa agctcaaccg gtgaaaatcc aatctgcttg ctttggtcat ctacccgcag
      841 gaagataggc gttttgcctc cattgatgtc cacacccata acgacgccat tccctactgc
      901 tttggagata acgatgctgt gccccttaat gacgccataa tcgcccgaag gcccccagtc
      961 tttatagaac tggctatagt catagggttt gtactggtca ggatgctggt cccagttctg
     1021 gtcgtgggtc cacagagcat aggccttttt gtagtcctgt gattccagtg ccgaaaagaa
     1081 tttgttcacg cgatgctcgg caggaatggt cctgaaaaac cagccatgcc cggtgacaaa
     1141 gccaagtacc cccgtcagcc cgataactac aatgacgatc acaatcgaaa ccacaatgtt
     1201 gcgatttcgc cgggccctgc gggcatcata ggcaggcgca tcgagaaggg tcatggggca
     1261 cagctctcct tttttgtttt aacaccacca gagtaccgga tgtttcgcgt caccgtgaat
     1321 actgcgtcct gtccttctcg gcgcggcgct ccagagcaag cgcaatcagg cggtcaatca
     1381 gttccggata cggcaccccg gaagccgccc acagcttggg atacatgctg atggcagtga
     1441 atcccggcag cgtgttgatc tcattcagat agatgcgtcc tgattttccc ggctccatca
     1501 gaaagtccac ccgcgccagc cccgagcagt cgcagacttt gaatgccgca actgccatct
     1561 gctgtacctg ttttgtctgc tgctttgtaa gctttgcggg gatgatgagg tctgagcctt
     1621 cactcaggta cttggcttcg tagtcgtaga actcttttac cggaactact tcgccaacga
     1681 ccgaggcctt tggactatca ttacccagca cggcgacttc gagttctcgg gccttgcccc
     1741 gtttaccgcc aactccctgc tccacgacaa tcttgcggtc gtagtttgct gccatctcaa
     1801 tggcaggccc aagttccttg cggtcgtgcg ccttgctaat cccaacagaa gagcctaagt
     1861 tcgcaggctt cacaaaaacc ggatacctca gtgctgcttc aatccgggct gtgatttttt
     1921 tacgggagga ttcccactcg ccgcgcagaa atgtgacgtg ctttgtgatg ggcagcttag
     1981 ctgaagcaaa aagccgcttc atcacgtcct tgtccatgcc ggtggctgag cccagtaccc
     2041 ctgagccgac gtaggcgatg ccggcaagct caaacagccc ctggatggtg ccgtcttcgc
     2101 caaatgtccc gtgcagaacg ggaaagatga cgtccacgct cagcgcctgg tgcgccggat
     2161 ggcgctcagt ttcaagcgga accagagagc ctgtttttga ttctgagggc acaggcggaa
     2221 caattaccgc ctctcctttg gccagaacag ctgccggggc tgtcgcttcc gggtcgcctg
     2281 cacgaaaatg gcgccccgga acgggttcac ctgcgagcag ccgctcagca tcggtggccg
     2341 tgacccagcg gccctctttg gtgatgccga taggaatcac atcgtatttc ttacggtcaa
     2401 tggccttgag caccgaggcc gctgaaagca aagagacttc atgctcgccg ctgcggccgc
     2461 caaaaagaac acctacacgc aatttggaca tactcgataa cccgagtgta tcgtgacgcg
     2521 ctgactgtat gcataagcat aggaatgatg acagtccgtc cccataccca cattggccgc
     2581 aaagtgttgc ggcctggctt cttgctggtg tggtgcccca gcgattgggg ggccatggaa
     2641 cctagagaat cttctttccg aatgcggaag agatcaactc gactgcgagg tctgctgttc
     2701 tgttgtgctc gtcaatgaca gggttgacct cgaccacctc caggctgagc atgcgtccgt
     2761 ggtcggcgat gatttccatc gccaggtgtg cttcgcggta agtcgcgccg ccccgtaccg
     2821 gagtaccgac ccctggagca tcttccgggt ctacccagtc catgtccaga gagacgtgat
     2881 atcccgccgt gccccgtcct gccgcgcgca acgcctcttc catcacggcc ctcatgccac
     2941 gctcatcaat atcgcgcatg gtgtagacct cggtgagtcc cgcacgtctg atgttttctt
     3001 tttcggtggc gtcaatgtcg cgcacgccaa tcagcactgt gttttccggc aggaccttcg
     3061 gcgaaaagcc gcagatattg gccagcgaat ctggccccag ccccagcagc gcagccagcg
     3121 gcattccatg aacattgccg ctgggcgaag tctccggcgt gttcaggtcc gagtgagcat
     3181 ctatccagat aaggccaacc ttctggtttc tgcgccgata aaactcagcc actccggaca
     3241 tgctgccagc ggccaccgaa tgatcaccgc caatgatgac aggggtcttg ccttcttcga
     3301 gtgcctgaat cacgtgctcg gcggctctgg tacaggtctc tgtgatctct ttcagatacc
     3361 gtgcgctctc ctcgccaacg tgtctggtct cggcaatagc aacgccaatg tttccaccat
     3421 cggtaacgtt gtggccgagt gcttcaaggc gcgcttccaa cccggcaacg cgcacagcag
     3481 acggccccat gtccacgccg cgccgcgaag cacccagatc cagaggtact ccaatgatgc
     3541 agatttcgcg tggcgggagc gtctggactc cgctatgtga ggctgcaagt gtttcaccaa
     3601 tcttagacat aggtcgttct ttcatccaca tcccttcagg atgtgggaca accggttttt
     3661 tccagatgcc gggtgccgga gaaattttac ggataaatcc ggtttagtgt cctggcaaag
     3721 ggaatcgtct cgcgcacgtg ctccagaccg cagatccacg ccaccgcgcg ctcaatcccc
     3781 attccgaaac ctgagtgcgg taccgaacca taccgccgca aatccagata ccactggaaa
     3841 gcatccatcg gcaggttgtg ctcttcaatg cgcttcttca gcaggtcaaa cgatgcgatg
     3901 cgctgcgacc cgccaatgat ctcgccatag ccttcaggtg ctagtacgtc cacgcagagc
     3961 gcatactttg ggtcctgcgg atcaggttcc atgtaaaacg ccttcacctc tgccgggtag
     4021 cggtgtacca ttaccggacg gtcaaattgc gatgagatgt aggtttcatc cggggagccg
     4081 aaatcgttgc catattcaaa cggctgttcg agcaggcctt tcgcgtgcgc atctttcagc
     4141 atctgcgcgg cttcatcgta gctgatgcga gggaacgggg ccttgattgc ttccagtttg
     4201 ttcgtgtctc gtccaatcac tttcaggtcg gcggcgcggc gcgtcagcac gcggtctacg
     4261 ataaagctga tgaattgctc ggccagcccc atcaggtcat ccagtgtggc gtaggcccat
     4321 tccggctcga ccatccagaa ctcggtcagg tgccgccgtg tcttcgattt ttccgcgcgg
     4381 aaggtcggcc caaagctgta gaccttgccc aaagccatcg ccgtgctttc aatgtagagt
     4441 tgtccgctct gcgtcagaaa ggcctcgtca ccgaagtact ccaccggaaa cagcgtgctg
     4501 gtgccctcac aggctgcggg cgtcaggacg ggcgaatcgg tcagcacaaa gccgttgtcg
     4561 tccagaaaat ctcgtgcagc tttgatgatt tccgcacgaa tgcgcaagat tgctgactga
     4621 cgcggtgtgc ggatccacag atggcggtgc tccatcagga aatccgtccc gtgctctttg
     4681 agcgaaatcg gaaacggcgt ttcttcagac acgcgctgca cggcttcgac attctcgaca
     4741 tcaagctcat agccgccggg agcgcgcttg tccgcgcgga ccttgcccgt aacaatgacg
     4801 cttgactcct gcgtcagtcc ctttaccgtc tcaaagacct caggcgtaac ggcagctttg
     4861 ggcactacgc cctggatcgt gcctgttcca tcgcgaaaaa taggaaacag tagctttccg
     4921 ctctcacgca ggttgtagag ccagcccttc agcgtgactg acttgccttc gtgctctccg
     4981 acgcgggcga tggtcgaaac aggcgcttcc gtggttgcag caacagtggt tggttcagac
     5041 atagcaatct tcagcggcaa aagccgaacc atccagtgta ttacggagcg ttgtcgaaaa
     5101 gcgtgggcct catttcgatt ggcaggggcg tgcccggtgt aaggcgctgc gtctggccat
     5161 ccagattatg tgcatagcta aagccaccct gcggaggcgt taccgttgct gtcgcaccga
     5221 ttttcgcagt ccacgcaatc agcccacgct ggccctgctg tgtagtgatg gggcaactcc
     5281 atactcctcc ctgcggcctg cagggaccgt tgaagacccc tccatgcagc catccatgga
     5341 ctacgaccag agcgcgtccg gctggcgtaa gtccaggtgg gatcacagga tcattggggc
     5401 attggtgcat ctcaggagag ctttgcggat agccaaatcc gatcagcgtg ccgttgcact
     5461 ggtggtcggc ttcgtaccag agatttacac ctacgccctc attcgccagc agaatcgttt
     5521 cccgcgccag aaatgcctgc cgcatccttc ccgtgatttc gtccgcatag ccattggcat
     5581 ccacaccgtg aaagaggttc ttgttcccgt accagccgga ttccgtgtcc cagaccggcg
     5641 tggaaggaga cagaaaggac tgccgctttg ccaacacagc gcgcactctt ccaggctggt
     5701 ccacaatcgc atattggcaa gagacattcg gcacgctgaa ctgcgcgcac ttggcatcgc
     5761 cctctgacac attggtttcc ggcatcggta cagggaaaac attcgtgcgc gatgggtacg
     5821 cgtgaaagct gacgatatcc ggaagcgatg cattcgggat atggtgccac gcatccagaa
     5881 actctcccaa agctttgtcg aattctccag aaccggctgg cccgtctccg gaacgcccga
     5941 cgcctccggc agaagtggat ccgccaatca ccttgcagtc accgcaataa atgcgcacga
     6001 taatcgccat atcattcgcc attttggcca gatgactcag cgagtcgtcc cagaaaatgg
     6061 gcgcgttgaa ctcattccag gcctcaaagt aatggatatg acacctgccc gtcagcggac
     6121 tggagggagc ggaagaaaca ccgcagttct tctgcattag cgccgtcacc cactctttcc
     6181 agatgcaatc gccgtccgga ctgacaacgc catcaagagg cgctttgcag gtctcattct
     6241 tctggtctat atcgagtggc gcgcgctttg caggccccgg ccccggttct cccgcggccc
     6301 aggcgggcac tgtattgaat gtatagatga gctgcgtatg gttcttctca gccaccggca
     6361 cccatcctgt ttccggagta aacagattgc caaacgtagg actgaagttt ccgcgtgacg
     6421 tttcaatctg ataccagcgc tctccggcat tggagcgaat gatgaccggg ttcggcagcc
     6481 caaacttcga tggccagttt ttggcgtgca gaatataagc gttctcgatt gaggcgtcgt
     6541 caatcgtgag cggatatgtg actgtctggg ccattccgca ggcaaagaaa gcagccacgt
     6601 ggacgaaaag caacatcaca gatcgcatga tgcatttcct catcatgcaa gcatcctaac
     6661 tcttttatgg attcacagcg ccatctgctc gcggaagagc tgcgactggc ggcgcgaaag
     6721 cccgacgcgg gtgccgtctt tgagcacggc tgtcaggctg aggtcaggct ccagttccat
     6781 ggattcaacc cagcgcaggt taattacctg attgcgattg gcacggaaga agtgctggct
     6841 gcccagccgc tcatgcagcg cctggaggct gcgcaggacc agcgggcggt gctctccaaa
     6901 acagatgcgc gtgtagttgc cttcgctctc aagcagacgg atgtcagcag ggcgcaccat
     6961 ccaggcccgc tcgccttcgc ggatgaaaaa ctgctgcggg gcctcgcgcg gcagag
//
LOCUS       DASRBV010000158         8025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_160445, whole genome shotgun sequence.
ACCESSION   DASRBV010000158 DASRBV010000000
VERSION     DASRBV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160445"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFA02_06045"
     CDS             <1..293
                     /locus_tag="VFA02_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZU09448.1"
                     /translation="NGVPASSIVEEDQAHDTIQNIYYSDKIMEAHGWHSAEVISSPYH
                     LPRTALILGSYPQLQWETHPAHWPPEYSLWRRIKLDWKEALSCFRIRIHTLA"
     gene            complement(345..2792)
                     /gene="lon"
                     /locus_tag="VFA02_06050"
     CDS             complement(345..2792)
                     /gene="lon"
                     /locus_tag="VFA02_06050"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZU09449.1"
                     /translation="MASDFLSVIHPSDPRQREGRNEGRVLPVLPVRDTVLFPHAVLPL
                     TVGRESSIQLIQSLGEEKTIVVVAQRDAHQDTPKPADLHIFGTMATVHKVVKMPNQSL
                     FVFTEGTERVKLGEFTQVEPFMTAHVDPVHDVPAQPGPELEALQRNILAQFQQIVAAS
                     PTLSDELSTIAAQMEDPGRLSDFVTANLPFLATPDRQELLETPDVHTRLDKLHKHLVK
                     EIEVQQLRNKIQSEVQDQVQQSQRDYYLREQLKAIQKELGEQDESQKDIEELRQKIEA
                     AGMPEETKKEAMKELNRLSRMSPMAADYSLTRNYIEWLAALPWSKSSGGEVDIAKAKE
                     ILDEDHYDLKKVKDRILDYLAVRRLKPDMKGPILCFVGPPGVGKTSLGRSIARALDRK
                     FQRISLGGMHDEAELRGHRRTYIGALPGQIIQNLRRAGANDPVFMLDEVDKLGRDFRG
                     DPASALLEVLDPEQNNTFRDNYLDQPFDLSKVLFICTANMLDTMPAPLLDRMEIIELQ
                     GYTEEEKRHIAFRYLIPRQIKENGIQPENIEFPEESVSYIVRHYTREAGVRKLEQLIG
                     TICRKQARRIAEGKMEKLVVTQEVIHEFLGGIKVRVDTEIAERTRRAGVAVGLAWTPA
                     GGDVLFVEANRMKGKGGFTMTGQLGQVMQESMQAALTWVRSNGKALGLEEDFMKDIDL
                     HIHVPAGAIPKDGPSAGVTMATALVSLLTDTPVRPLTAMTGEITLSGNVLPVGGIKEK
                     FLAAKRAGVKDVVLPAENKLNVEEDLTPDQTDGVNIHYARHIEDVLAVALPKSRAEEK
                     KDAQVREDVLQEAAAQA"
     gene            complement(3095..3919)
                     /gene="pheA"
                     /locus_tag="VFA02_06055"
     CDS             complement(3095..3919)
                     /gene="pheA"
                     /locus_tag="VFA02_06055"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZU09450.1"
                     /translation="MKVAIQGEPGSFSHEAAGKMLREATILPCAFSADVFRSLVEGKA
                     DAAVIPIENSLAGPVTEHYDLLLQNPVAIEREGLLRIRHNLIAVPGTKMETIERVYSH
                     PVALAQCRAFFAKHPHMQASPFYDTAGSVKHVMETRDPGAAAIASTRAAEHYGAQILT
                     AGIEDNQENYTRFFLVRRRENVRPEPDADKVSLAFTVENRPGTLVAALQVFAGQGTNL
                     TKIESRPVQGRPWEYVFYADYQLSPGARTDAALELLAQHCSMVKELGRYRSGLKLE"
     gene            4010..4159
                     /locus_tag="VFA02_06060"
     CDS             4010..4159
                     /locus_tag="VFA02_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZU09451.1"
                     /translation="MLWTIFVILLVLWLVGLVSFHAIGWYIHILLVIALVVLIIQLIT
                     GRRPV"
     gene            4177..4452
                     /locus_tag="VFA02_06065"
     CDS             4177..4452
                     /locus_tag="VFA02_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZU09452.1"
                     /translation="MNKHHISGKMDQATGKVKEKMGTALNNQRLANSGVADQIKGAAK
                     EVWGNVKDATKDEARRQATEARHRTTEKMQEMKNKANQKVQEFRKSA"
     gene            complement(4511..5377)
                     /locus_tag="VFA02_06070"
     CDS             complement(4511..5377)
                     /locus_tag="VFA02_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZU09453.1"
                     /translation="MAFLRSVGTTLEMIKWEHSVFALPFALTGAMLAAHGWPRPAQLL
                     WIIVCMVTARSAAMAFNRWADADLDAVNPRTANRAIPAGLLSRGFVGFFTIVMAVLFV
                     LAAAQLNRLTLLLSPVALAIVLSYSYTKRFTRWSHLVLGLSLGIAPSAAWIAVRGTLD
                     WRILVLTVAVLFWVAGFDILYACQDAEHDRSHHLYSIPATVGIPAAFWLVRVMHAVML
                     GLLLWLVEIFALGPVALLGVALVAGLLLYEHLIVSPKDLRRLNAAFFTMNGVISIVFF
                     VFVAAALLLRQS"
     gene            complement(5466..6278)
                     /locus_tag="VFA02_06075"
     CDS             complement(5466..6278)
                     /locus_tag="VFA02_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZU09454.1"
                     /translation="MRKAPRSLVPALAAVLLSVSPSAIAPHSAVAVAQSAQDAQIKAD
                     IINNALNKSKFKNVQVNVQNGVAILTGTVDVYDTKIDADRRVHRIKNIKGVENEIEVA
                     GPEVPDQQLREKIAKAIEYDRVGYWGNSRIISDVTPFNAISVSVHNGVVTLGGHAYGP
                     VDADSAVAVAANTPGVKDVINDIQVDPVSPMDDRIRIAVFRAVYGFPSLNKYAIDPGK
                     PIRISVQNGHVTLYGVVDSQADKETAGIRANSVPGVFSVTNNLQVAGQPEKK"
     gene            complement(6426..>8025)
                     /locus_tag="VFA02_06080"
     CDS             complement(6426..>8025)
                     /locus_tag="VFA02_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZU09455.1"
                     /translation="QTQAQAQLTNLDIDPFLKMFEVEGVQGHSSIAGLVSVSGPLRAP
                     RQMQGEAQISQLSVSLGGVPLKSEGPLHAQLRNGNLHLDPLHITGDDTDLRAQGDAGI
                     LEERHPMNFQAQGSINMKLLQTLRSDVDSSGQVTFTMNAGGTFKNPALTGQVKLSNVA
                     MSVESFPNGLSQLNGTLQFTQDRLQVQSLTGTTGGGQVNVTGYITYQQGIYADLTATG
                     KDIRIRYPAGISSMIDTKLRLQGTQSAFMLSGNVQLTRFTINPNLDFASFNRVSAISA
                     APPDPNAFSNRVRLNIHITSSPDLDFQNSYAKLAGDVDLRIRGTVAQPSVLGRITITE
                     GSATFAGTKYELQHGEIFFSNPVRIEPVIDIDATTHVEDYDITIGVHGTPSNLTPTFR
                     SEPPLTQQDIFSLLAMGRTQEEQQIYSQQAQQAGVNTTADAILGGALNATLSSRIQKL
                     FGGGSVKIDPSWVGSLGNSTARITVSQQVSKNATLTYATNINSTAQQLIQAEVNVTPT
                     VSILAVRDESGVFSLLFKVHRRYR"
BASE COUNT         1799 a         2371 c         2057 g         1798 t
ORIGIN      
        1 ccaatggggt acccgcttca tccatcgtgg aggaagacca ggcccacgat accatccaga
       61 acatttacta ctcagacaaa atcatggaag cgcacggctg gcattcggct gaagtcataa
      121 gctcaccgta tcatctcccg cgcaccgccc tcatacttgg aagctatcca caactgcaat
      181 gggagacaca cccggcacac tggcctcctg aatacagcct ctggaggaga atcaagctgg
      241 actggaagga agcgctgagt tgcttccgca ttcgcattca cacgttggct tagaaaataa
      301 aggcccgcat cttatcgcgg gcctgttctg atgatctgat tgtattacgc ctgggctgca
      361 gcttcctgca acacatcttc cctcacctgt gcgtccttct tctcttccgc ccttgatttt
      421 ggcaaggcca cagccagcac atcttcaatg tgcctggcat agtggatgtt cacgccatct
      481 gtctggtccg gtgtcaggtc ttcttccaca ttcagcttgt tttctgcagg aagaaccacg
      541 tctttcacac ctgcccgttt cgcggccagg aatttctctt taatgccgcc cacaggcaat
      601 acatttccgc tcaatgtgat ttcacctgtc atcgccgtca gtggacgcac tggtgtatcc
      661 gtcaacagcg aaaccagtgc cgttgccatc gttacgcccg cagaaggccc gtccttggga
      721 atcgcgccag ctggaacatg gatgtgcagg tctatgtctt tcatgaagtc ctcttccaga
      781 cccagtgcct ttccattcga ccgcacccat gtcagcgcgg cctgcatgga ttcctgcatt
      841 acctgtccaa gctgtccggt catggtaaat ccacccttgc ccttcatgcg gttggcttct
      901 acaaacagaa cgtcaccgcc cgctggggtc caggccagac ctacggccac tcccgcgcgc
      961 ctggtgcgct cggcaatctc cgtatccacg cggaccttga tgccacccag gaactcgtga
     1021 attacttcct gcgtaacaac cagcttttcc atcttgcctt ctgcaatgcg gcgcgcctgc
     1081 ttgcggcaga tcgtgccaat cagctgctcc agcttgcgca caccggcttc gcgcgtgtaa
     1141 tgccgcacga tgtatgagac ggactcttcc ggaaactcga tgttttccgg ctgaataccg
     1201 ttctccttaa tctgccgcgg aatcagataa cggaacgcga tgtgccgctt ctcttcttcc
     1261 gtgtatccct gcagctcgat gatctccatg cggtccagca gcggagcagg catcgtgtcc
     1321 agcatgttcg ccgtgcagat aaacagtacc tttgacaagt caaacggctg atcaagatag
     1381 ttgtcgcgga acgtattgtt ctgttccgga tccagcactt caagcagtgc cgacgcagga
     1441 tcgccgcgga aatcccgccc cagcttgtct acctcatcga gcatgaaaac cggatcattg
     1501 gcgcctgctc gccgcaggtt ctggatgatc tgccccggca acgcgccaat atacgtgcga
     1561 cgatgcccgc gcagttcggc ctcatcgtgc atgccgccga gagaaatgcg ctggaattta
     1621 cggtcgagtg cgcgcgctat actgcggccc agcgaggtct tccccacgcc aggaggccca
     1681 acgaagcaca gaatcgggcc tttcatgtcc ggcttcagac ggcgcaccgc aaggtaatcc
     1741 agaatgcggt ccttcacctt cttcaggtcg tagtggtctt cgtcgaggat ctccttcgcc
     1801 ttggcaatgt ccacttctcc gcccgaagat ttcgaccacg gcaacgccgc aagccactca
     1861 atatagttgc gcgtcagcga gtagtccgca gccattggag acatgcgcga gagccggttc
     1921 agctctttca tcgcctcttt ctttgtctcc tccggcattc ctgcggcctc aatcttctgc
     1981 ctcagctctt caatgtcctt ctggctctcg tcctgctcac ccagttcctt ctggatggcc
     2041 ttgagctgtt cacgcaggta gtaatcgcgc tgcgattgct gcacctggtc ctgcacttca
     2101 gactggattt tgttgcgaag ctgctggact tcaatctctt tcaccagatg cttgtgcaac
     2161 ttgtccagcc gggtatggac gtccggggtc tccagcagct cctggcggtc aggcgtggcc
     2221 agaaacggca gattggccgt aacaaaatcg ctgagcctgc ccgggtcttc catctgcgcc
     2281 gcaatggtcg aaagctcgtc agaaagcgta ggagatgcgg ccacaatctg ctgaaactgc
     2341 gccagaatat tgcgctggag cgcctccagc tccggccccg gttgcgctgg aacatcatgc
     2401 accgggtcta catgggccgt cataaacggc tcaacctggg tgaattcgcc cagcttcacg
     2461 cgctccgtgc cctctgtaaa cacaaagagg ctctggttcg gcattttaac gaccttatgc
     2521 acggtcgcca ttgtcccaaa tatgtgcaga tcggcaggtt ttggagtgtc ctggtgggca
     2581 tcacgctgtg ccaccaccac aatcgtcttc tcctcgccga gtgactggat caattgaatc
     2641 gagctttcgc gtcccaccgt aagcggcagc acggcatgag gaaacagtac ggtgtccctt
     2701 acaggcagga caggcaatac ccgcccttcg ttacggccct cgcgctgcct ggggtcgctt
     2761 ggatgaatta cgctgagaaa atcgctagcc atgagtgtct ctttatcaga ttatatgatc
     2821 tgaatacgat gaatttcttc cgtttgattc atctccagca aaaaaggtcg caaattgcag
     2881 cccgcatgac cgcttgcaac gcctttcctt tcttggatgc aagccctcgg tcacttcttt
     2941 ccgctcatca gccctatcgg caaatccacc tgggagtgaa gcggaaagag gcaggaaaac
     3001 tctgttctca gctcttgggg ggatggaatg caacctactg attcgggacc cgttcatctg
     3061 accctggacc tgcccgaaaa cgccggaata gaccctactc cagtttcagc ccggaccggt
     3121 accgccccag ttccttcacc atcgaacagt gctgcgccag cagttccaga gcagcatccg
     3181 tccgcgcccc gggagaaagc tgataatcag catagaagac atactcccac ggccggccct
     3241 gcactggacg cgactcaatc ttggtcaggt ttgttccctg cccggcaaaa acctgaagtg
     3301 ccgccaccag cgtccccgga cggttctcca ctgtaaaggc aaggcttacc ttgtctgcat
     3361 ctggttcagg acgaacattc tcccggcggc ggaccagaaa gaagcgcgta tagttctcct
     3421 ggttgtcctc aattccagcg gtcagaatct gcgcgccata atgctctgcc gcccgcgtgc
     3481 tggcaatggc tgcagcgccg ggatcgcgtg tttccatcac gtgcttcacg cttcctgccg
     3541 tgtcgtaaaa gggcgatgcc tgcatatgcg ggtgctttgc gaaaaacgcc ctgcactgtg
     3601 ccagtgccac cggatgcgaa tacacacgct caatcgtttc catcttcgtt ccaggcaccg
     3661 ctatcagatt gtgccggatg cgcagcagtc cctcgcgctc aatggcaact ggattctgaa
     3721 gaagcagatc atagtgctcg gtcaccggcc cagccaggct gttttcaatc ggaatcacgg
     3781 cagcatccgc cttcccttct acaagtgagc ggaagacatc ggcggaaaag gcacagggca
     3841 gaatggtagc ctcgcgaagc atttttcctg ctgcttcgtg actgaaagac cccggttccc
     3901 cctgaatggc aaccttcatt gagcggttct ccttatgatt ttgtttccat cttgcggcaa
     3961 cccggatgcc gtcaagcgca ccataccaca ccataggaga acaaagttta tgctatggac
     4021 catttttgta attcttctgg tgctttggct ggtgggcctg gtcagctttc atgcgattgg
     4081 ctggtacatc catattctcc tggtcatcgc gctggtcgtg cttatcatcc agctgatcac
     4141 cggtagaagg ccagtgtgat ccagaaagga ggagcgatga ataaacacca catcagcggc
     4201 aaaatggacc aggcaaccgg caaggtaaag gagaaaatgg ggacggccct caataaccag
     4261 cgtcttgcca acagcggcgt cgccgatcag ataaaaggcg cggcgaaaga ggtatggggc
     4321 aacgttaagg atgcaacgaa ggacgaagcc cgcagacaag ccaccgaggc ccgccaccgc
     4381 accacggaaa aaatgcagga gatgaagaac aaggccaacc agaaggtcca ggaattcagg
     4441 aaatcggcgt aaatgcccaa tagctccgca actgaaccgg cgcgaaggaa acatcttcct
     4501 tcgcgttttt tcacgattgc cgcaacagaa gggccgccgc aacaaaaaca aagaagacga
     4561 tggagatgac accattcatc gtaaagaatg cggcattcag tctccgaagg tccttcggtg
     4621 aaacaatcag gtgctcataa agcagcagtc cggccaccag cgcaacaccc aacaatgcca
     4681 ccggccccag cgcgaagatt tcaaccagcc agagcaacag ccccagcatc actgcatgca
     4741 tcacgcgtac aagccaaaat gccgccggaa tgccgactgt ggcaggaatg ctgtaaagat
     4801 gatgactgcg gtcatgttcc gcatcctggc aagcataaag aatgtcgaat cccgccaccc
     4861 agaaaagaac ggccaccgtg agcaccagga tccgccagtc cagcgttccg cgaacagcaa
     4921 tccacgcagc cgaaggcgca atgcccagtg aaagacccag caccagatgt gaccaccgcg
     4981 taaagcgctt tgtataggaa taagaaagca caatcgctag tgccactgga gacagcaaca
     5041 gcgttagccg gttcagttgc gcggcagcca gcacaaacag caccgccatc actatggtga
     5101 agaagcccac aaatccacgc gacaacaaac cggcaggaat ggcgcggttg gccgttcgcg
     5161 gattcaccgc atccaggtcc gcgtcggccc agcgattaaa ggccatcgcc gccgaacgcg
     5221 ccgtaaccat acagacgata atccagagca gttgggcagg acgcggccag ccatgtgcgg
     5281 ccagcatggc cccggtcaat gcaaaaggga gtgcgaagac agaatgctcc cacttaatca
     5341 tttcgagcgt cgtgcctacg ctgcgcaaaa atgccatcgt tgatagatgc ctctcctcca
     5401 gtttaaagca caaataaagc ggccccgctt tccggcgagg ccgcttatcg ttcagaaaag
     5461 aagcgctact tcttttccgg ttgccctgcg acttgcagat tgtttgtcac gctgaagacg
     5521 cctggaacag agttggcgcg aatgccggct gtctctttgt cggcctgact gtcaacgaca
     5581 ccatagaggg tgacgtgtcc attctggaca gaaatgcgga tcggtttacc cggatctatc
     5641 gcatacttgt tcaggctggg gaaaccatat accgctcgaa atactgcaat ccggatgcgg
     5701 tcatccatgg gagaaacggg gtctacctga atgtcattga tgacatcttt gactccggga
     5761 gtatttgcag ccaccgccac ggctgaatca gcatccaccg gaccgtatgc gtgaccaccc
     5821 agcgttacca ccccattgtg cacgctgact gaaatggcgt tgaagggggt gacgtcgctg
     5881 atgatcctgc tattacccca atacccgacc cgatcatatt cgattgcctt ggcaattttt
     5941 tcccgcaatt gctggtccgg cacttcaggc ccggccactt caatttcatt ttcaacgccc
     6001 ttgatgttct tgatgcgatg aacacgccgg tcagcatcta tcttggtgtc atacacatcc
     6061 accgtgcccg tcagaatggc aactccattt tgcacgttta cctgcacgtt cttgaatttt
     6121 gacttgttca gcgcattatt gatgatgtca gctttgatct gcgcatcctg cgcgctctgt
     6181 gccacagcaa ccgctgaatg cggcgcaatg gcagaaggag agacactcag caaaaccgct
     6241 gccaaagctg gaacaagact gcgtggtgct ttcctcatgt caatctttct cctgggcggg
     6301 ctgcgcttta ctttctggca aacataacaa cagctccagc cgagtcaaag gcagcctgga
     6361 tgtaaactac ggacatattg tactcatgtt tgatgcagaa aaggccaatt ttctgcctgt
     6421 gtgcttcacc gatagcgcct gtgtacctta aacagcagac tgaatacgcc tgactcatcg
     6481 cgcacggcaa gaatagacac cgtaggggtc acgtttactt ccgcctggat aagctgctgg
     6541 gccgtagagt tgatattggt tgcatatgtc agcgtcgcgt tttttgatac ctgctgcgaa
     6601 acggtaatac gtgccgttga gttgcccagc gagcccaccc acgatggatc aatcttgacg
     6661 ctgccgccgc caaacagctt ctggatacgg ctgctgagcg tcgcattcag cgctccgccc
     6721 agaatggcgt ccgcagtagt attcactccg gcctgctgtg cctgctggga ataaatctgc
     6781 tgttcttcct gtgtgcgccc catcgccagt agcgagaaaa tgtcctgctg tgtcaacgga
     6841 ggctcggagc ggaacgtggg cgttaaattg ctcggtgttc catgcacacc gatcgtgatg
     6901 tcatagtcct ccacatgggt cgtggcgtca atatcaatca ctggctcaat gcgtaccgga
     6961 ttgctgaaaa atatctcgcc atgttgaagc tcatattttg ttccggcaaa tgtggcactg
     7021 ccttcggtaa tcgtgatgcg ccccaggact gaaggctgtg ccacggtccc acgaatgcgc
     7081 agatccacgt cccctgccag tttcgcgtag gaattctgga agtccaggtc cggcgatgaa
     7141 gtaatgtgaa tgttcagccg gacgcgattg gaaaacgcat ttggatcggg aggggccgct
     7201 gaaattgcag aaacacgatt gaaactcgca aagtccagat tgggattgat cgtaaaacgc
     7261 gtcaactgaa cattgccgct cagcataaag gcgctctgcg ttccttgcag gcgaagcttc
     7321 gtatcaatca tggaactgat gccagccgga taccggatgc gaatgtcttt tcccgtggca
     7381 gtcagatcag catagatacc ctgctgatat gtaatatatc cagttacatt tacctgtccg
     7441 ccacctgttg tcccggtcag gctctgtacc tgcagcctgt cttgcgtgaa ctggagcgtg
     7501 ccgttcagtt ggcttaatcc attcggaaag ctctcaacac tcatggccac attcgacaac
     7561 tttacctgtc cggtcagggc ggggtttttg aaggtacccc cggcattcat cgtgaacgtg
     7621 acctgtccgg aagaatctac atccgagcgc agcgtctgca atagcttcat gttgatggag
     7681 ccttgcgcct ggaaattcat tggatggcgc tcttccagaa ttccggcatc gccctgtgcc
     7741 cgcaggtctg tgtcgtcgcc tgtaatatgc agcgggtcga gatgcagatt gccattgcga
     7801 agctgtgcat gaagaggccc ttcgctcttc agcggaactc ctcccaggga tacagaaagc
     7861 tggctgatct gcgcttcacc ttgcatctgc cggggcgcgc gcagtggccc ggaaacagac
     7921 accaacccgg caatcgagga gtgtccctgc accccttcca cctcgaacat cttcagaaac
     7981 gggtcaatgt ccaggttcgt aagctgtgcc tgtgcttggg tctgg
//
LOCUS       DASRBV010000159        13459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_363519, whole genome shotgun sequence.
ACCESSION   DASRBV010000159 DASRBV010000000
VERSION     DASRBV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13459
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363519"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFA02_06085"
     CDS             complement(<1..372)
                     /locus_tag="VFA02_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09456.1"
                     /translation="MAIPIKVEYAAQRRIRERNTQLYRLAHWPIWIWVFFLAPGWLTF
                     DLFAKGGSRWNLLWLAAVLLGTGIAAYRGKLPGSEPCPYILRFDEDRPNPLYRKVCYT
                     FAWNAVLSFALLNLAGLIDAVR"
     gene            complement(429..1166)
                     /locus_tag="VFA02_06090"
     CDS             complement(429..1166)
                     /locus_tag="VFA02_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU09457.1"
                     /translation="MFFTVLEALLHFFVLRLRKGRPTLYDRAQWLHETSILVLRRIGI
                     TVRSEGPVPQTGLIVSNHLGYLDIPFHAALGPRIFVSKDDVRSWPVFGLLARLGGTIF
                     VKRGIRASATEAAIEIEFALRSGVPVMLFPEGTSTDGTTLLPFHAFLFEPAVETASPV
                     SAAAIRYEAPGTDERELCYYADVRFAPHLLETLGRKHVSGRLRFDGKPRIYSHRKRAA
                     NDCWERVARLRLRAEYIPHNDMAEQWH"
     gene            complement(1169..2050)
                     /locus_tag="VFA02_06095"
     CDS             complement(1169..2050)
                     /locus_tag="VFA02_06095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HZU09458.1"
                     /translation="MARLLHHDGVATQLRPARPALVPGHFPQLPSVFSPRIFAAAGKY
                     QMRLAISEQEREAACRLRFRVFNLELGEGLRSSYSTGLDMDQFDYVCDHLLVEDRASN
                     EIVGTYRMQSGNSAKQRLGYYSAQEFDFSPYEAIRSQILELGRASIAKEHRSSEVLTL
                     LWRGIAQYCKLHRLRYLIGCSSLNSQDPGEGWAVYEQLRPFLAAPESLTVPNPSYRLP
                     EKISTGDLAPRIPRLLKTYIGIGARICGEPAWDREFGTIDFLTLLDLQQLTPAARDRF
                     LLDGEHQATTAAASCAS"
     gene            2402..2623
                     /locus_tag="VFA02_06100"
     CDS             2402..2623
                     /locus_tag="VFA02_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09459.1"
                     /translation="MSREEIIAAIDEEISRLEKVRALLEGAGTTRIFAGAFANGRKKR
                     HLSAEARRRIAEAQKRRWAKQKKQPVSSQ"
     gene            2655..3149
                     /locus_tag="VFA02_06105"
     CDS             2655..3149
                     /locus_tag="VFA02_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZU09460.1"
                     /translation="MIKVKLLHPDARVPTVNYPGSDLGYDLYSIEDITLHPGVPVKVR
                     TGIALEAADGYGFVLGDRSSMAAKGITYAGGRIDAGYRGEILVCLINVNQPRYSLRVT
                     HDAQGRPTDVDLQQTDVSVTIRKGDKIAQMSPILPATHLPVLAVEELAESSRGAKGFG
                     SSGR"
     gene            3185..3973
                     /locus_tag="VFA02_06110"
     CDS             3185..3973
                     /locus_tag="VFA02_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU09461.1"
                     /translation="METGLKDRVAIVAASSQGIGKAAALAFAAEGAHLALCARQPGPL
                     DSVAREATDRYGVKVFTEAFDVSDDAAVRSFVKSAHDHFGHVDVCVTNAGGPPAKPFL
                     ATTLDEWERAWQLNLRSIVSFAQAVIPYMQKKKWGRIVTLTSYTVKQPVPELVLSNSI
                     RAGVMGLVRSLAGQFGPDGITVNNVGPGFTSTARAEELLEKRAAARNTNIEQIRQELL
                     QDIPVRRMATPEEVAAAIVWLASEQASCITGQTLLVDGGAYRGL"
     gene            4118..4741
                     /locus_tag="VFA02_06115"
     CDS             4118..4741
                     /locus_tag="VFA02_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09462.1"
                     /translation="MKPTRTTVLSMAIFALSTTLGLAQTASTQTQTQTDSKMTVNQRR
                     ENQQDRIANGVQSGQLTAAETKNLESREANLNREIKDDRKADDGHLTTAEKQQINHQQ
                     NNLSRSLYDDKHNANTAHFGNGEVGQRRENQQDRIAQGIRSGQLTAGETSRLENREQG
                     INQQIHADRQANGGKLTAGEKAQMNRQQNRASRAIYRNKHNGAHGPR"
     gene            5048..8545
                     /locus_tag="VFA02_06120"
     CDS             5048..8545
                     /locus_tag="VFA02_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09463.1"
                     /translation="MRGILILPLVLLCTVALHAQFRGSLRGTVTDPQGAIIPGATVTL
                     TNTDTNQSQTATTDNNGIYIFNGLPPAHFRLTVDASGFKQKVLEHVQIIPEQLNSLNV
                     QLEVGGAQQTVTVSGTTQALDSETATVSATVSSNQIQHLPSFNRDVFQLAQLTPGVFG
                     DGSQGGGGGTFNLPGNQGPGGSGGGQAGIFATENGPQIQTRGGQYETNGISIDGISTV
                     SAVWGGTSVITPSEDSVDSMKVVSNSYDAENGRFSGAQIQVTSKSGSNELHGSAFFKM
                     SRPGLNAYQRWNGVSSNFAGTAADRGVNRDNDRFNNYGGSLGGPIWRDKVFAFFNWET
                     SPLSAVNTAQGWYETSQFDSLTGIGPIAWKYLSYPGEAVSATGILQRACASIGLQEGV
                     NCRTTSQGLDVGSPMKNGIGLQDPTYGGNSNQPGVGGGLDGIADLAYFNSISPSKTSQ
                     NQYNGRLDVNVTQNDHLAFAIYWVPVDSTFYNGPVRSANLWHHSQINDAFSLIWNHTF
                     SPTLLNQARANAAGWRWNEINSNPQEPFGLPQANIDAIGTIGNQGGFQFFGAPGPSNF
                     NQWTYSYNDVLTKVLGRHNIKAGGELTRLYYLNNPVYAARPAFNFNNLWTFANDAPYF
                     ESGQFDSKTGIPFGNRQDNRENIWGGFVQDDYKIKPNLTINLGLRWSYFGAFYSKENN
                     LGVLRFGSGPNPLDGLNIRIGGGIYSPQKNNWGPQVGFAWQPKQANGNLVLRGGFGIN
                     YNQNEIAITANGNGNPPFVVQANFTCSYPYTNNPSCAGTGIVYQTATDVHSLFGYPPN
                     PNAVKTFGPDNLPLAGSAPIFVTGFLPNPKTIANYHYSLDMQYQLPLGLVASLGYQGS
                     QMRHLLIQNNWNAIAAARGLSLNPKVNFLDFYQNSGTGNYNAMIATLDHSFSHGFQAE
                     VMYTWAKAMDENSGPYSEDYYPYDTHAAYGRSDYNVANAVKIFGLWQPIFFHGSHSWI
                     EKVAGGWSLSGIWNWHTGFPWNPIYNAAGSLYYPGSGYGQLRPAARLRGYGTNTSNKT
                     FQQDTNPNFGGDGTKFFPGPTYVAATGSSFTDTVPAPVAGIQRNSLNGPNYNDVDASL
                     AKAFGLPGMPILGENAKFEIRADAYNLFNKLNINPSSIDNNLGSVAPNGTVTPNPDFG
                     VAGSALGSRTVQIQARFSF"
     gene            8629..10125
                     /locus_tag="VFA02_06125"
     CDS             8629..10125
                     /locus_tag="VFA02_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HZU09464.1"
                     /translation="MKFLHAVAVCATYIFLLLAQAFAQEQVNINAQAQTIPLPHFWEE
                     MFGSGRANLTLRESYRNDLRAVKKITDFHYVRFHAILHDENGVYNEDEHGNPVYNFQY
                     VDQIYDGLLKNSVRPVVEISFMPKKLAFNPDALHPFWYKQNVSPPKSMERWDDLIRHL
                     AQHLVDRYGIDEVSQWYFEVWNEPNIDFWNGIPRKQSYFELYAHTARDLKSVSPRLRV
                     GGPATAAAAWIPDFLKYTAENHVPVDFVSTHGYADDTVENLFGTHENIPMDDRVCRAV
                     AKVKGEIKASAMPGLPLLWTEWNVPGEMEARDTIYVGPALANTIRECAGEGVEALSFW
                     TFSDVFEEGGPIPAPFTGQFGLRAQGGINKPSFYAFALLHQLGNQLLPNASKNVIVTK
                     TPEGGLAIAAWNLVDPGEHGATKQITLVFQGLREDAPVSINRVDENHGNVLKAYAAMG
                     RPVYPTPEQVEQLNRETDLGVPEQRHLNHGKLTVDLTPNALLLIKIEK"
     gene            10183..12477
                     /gene="bglX"
                     /locus_tag="VFA02_06130"
     CDS             10183..12477
                     /gene="bglX"
                     /locus_tag="VFA02_06130"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HZU09465.1"
                     /translation="MSASVYAGAVLAQSARPSNAQLASPQIERKVDDLLKRMMLDEKL
                     GQLVQYNDAGYIAATAAQLAINPEARNKVDAMQLAAEGKLGSMLNTVGAEHTNAFQRA
                     AVEKSRLHIPLLFGADIIHGFRTIYPEPLGLAATFDPELVRELSRMSAEEATTAGIRW
                     FYSPMVDISRDARWGRTAEGAGEDPYLGAAMARAYVRGYQQDDLSKPDSVAACVKHFA
                     AYGAAEAGREYNTTDMSDIRLRQVYLPPYKAAVEAGAATIMSAFNALNGVPATANPYL
                     LQKILRDEWGFNGFVVSDYTAVMELQNHGIALTPAEAAKKAFLAGVDVDMMSHYYDTL
                     LPELIRSGQVPMNAVDEAVRRVLRVKFALGLFEHPYARGTEVTAAVPEHRALVRRAAE
                     ESFVLLKNDANILPLVQKAQKIALVGPLADNPADMLGAWSGGPQMKDVVTIRAALEAR
                     AKQYGGQVLYAKGTDIQGTSESGFAQAVQAAKQADVVVMALGESGTMSGEAGSRAHLD
                     LPGNQEQLLETIAQTGRPVVLLVFSGRPLVLTWAALHIPAIMEVWFPGTEAGNAIANV
                     LYGDVSPSGKLPMSFPWTVGQEPLYYNQFPTGRPPAPEMDLTRPPGPDSRFVSRYIDA
                     PNAALFPFGFGLTYTSFSYGKVSLSRNAVPLNEANHEDAKKLVIATATVTNTGTREAT
                     EVVQCYVRNLGASIEQPVRSLKGFARVTLKPGESKRVSFDLGFPELSFYNSEGKQVIE
                     PTHYTVWIGGSSLAKEEASFDVKQ"
     gene            complement(12481..13380)
                     /locus_tag="VFA02_06135"
     CDS             complement(12481..13380)
                     /locus_tag="VFA02_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU09466.1"
                     /translation="MRESLEFAIVWLLVRILGALPRSLARFIGAALGAVAFHTVPRLR
                     KVGLKNLELAFPEKSSAERQQILRKLYRNLGWLLAEFCQMSYYTPENTRNFIRYEGLD
                     RYLAARDKGKGVLIVTGHLGAWELSSFYHSLMGHPMSMVIRRLDNPKVDHLVNSIRCL
                     HGNRVLHKDDFARGLLAAMRKGETVGILMDTNMTPPQGVFVPFFGMLACTASGLARVA
                     LKTGAAVLPGFMLWEESRQEYVLHFGEEIQMVSTGDDEADTIVNTARCTAAIEACIRS
                     YPDQWLWVHRRWKTRPDGEPKIY"
BASE COUNT         3293 a         3451 c         3724 g         2991 t
ORIGIN      
        1 cctgacagca tctatcagcc cggccagatt cagcagcgcg aagctcaaca cggcattcca
       61 ggcgaaggtg tagcaaactt tgcggtagag cgggttcgga cggtcttcat cgaaacgaag
      121 aatatagggg caaggctcgg agcccggcag cttgccgcgg tacgcagcaa ttccggtgcc
      181 cagcagcacg gcggccagcc agaggaggtt ccagcggctg ccacctttgg cgaacagatc
      241 aaaggtcaac cagcctggcg cgagaaagaa aacccatatc cagatgggcc agtgcgccag
      301 acgatatagc tgtgtattgc gctcgcgaat ccgtcgctgc gcggcgtact ccactttaat
      361 gggaatggcc atgttgaccg cagtgtgtca ggggaacaga tggcgggtca agcggcgtat
      421 gctttctttc agtgccactg ttcggccata tcattgtgtg gaatatattc ggcgcggagc
      481 cgcaatcttg ccacgcgctc ccagcaatcg ttggcagcgc gcttgcggtg actgtaaata
      541 cgcggcttcc catcgaaacg aagtcttccg ctgacatgct tgcgacccag cgtttccagc
      601 agatgcggcg caaagcggac atcggcgtaa tagcaaagct cgcgctcatc tgtgcccggc
      661 gcctcatagc gtatagctgc ggcgcttaca ggagatgctg tttcgactgc gggctcgaaa
      721 agaaaagcgt ggaacggaag cagggttgtt ccgtctgtgc tggtgccttc aggaaacagc
      781 attacgggaa cgccggagcg tagtgcgaat tcgatttcaa tagcagcttc cgtcgcgctc
      841 gcgcgaatcc cgcgttttac aaagatggtc ccgcctaagc gggccagtaa cccgaacacg
      901 ggccatgaac gcacgtcgtc ttttgaaaca aagattctgg ggcccagagc ggcatgaaat
      961 ggaatgtcaa gataaccgag atggttggag acaatgaggc cggtttgtgg aacaggccct
     1021 tcgctgcgga cggtaatgcc gattcgccga agcacgagaa tggatgtctc atgcaaccac
     1081 tgggcgcggt cgtacaacgt tgggcgtcct ttacgcaggc gcagcacaaa aaaatgcagc
     1141 agcgcttcca gcactgtaaa gaacaagact acgatgcgca ggatgctgcg gcagtagtcg
     1201 cctgatgttc tccgtccagt agaaatcggt ccctcgctgc tggcgtcaac tgttggagat
     1261 cgagcagggt aagaaaatca atcgtgccga actcgcggtc ccatgctggt tctccgcaaa
     1321 tgcgcgcccc aattccaata tacgtcttca gaaggcgggg aatcctgggc gcgaggtccc
     1381 ccgtcgagat tttctccggc aatctatagg atgggtttgg aaccgtcaga gattcaggtg
     1441 ctgcgagaaa cggcctcagc tgttcataaa cggcccagcc ttcgccgggg tcctgcgaat
     1501 tgagtgatga gcagccaatc agatagcgca accgatgcag cttgcagtat tgtgcaatgc
     1561 ctcgccatag cagcgtcagc acttcgcttg agcggtgctc ttttgcaatt gaggcacggc
     1621 ctagctccag aatctggcta cgaattgctt cataagggct gaaatcgaat tcctgcgcgc
     1681 tgtagtatcc cagcctttgt tttgctgaat ttccgctttg catacggtac gtgcccacga
     1741 tttcgttgct ggcccggtct tcaacaagca gatgatcgca gacataatca aactggtcca
     1801 tatcaagacc agtggaataa gaagaccgaa gcccttcacc cagttccaga ttgaataccc
     1861 tgaagcgaag cctgcatgct gcctcgcgtt cctgctctga aatggcgaga cgcatctgat
     1921 attttcctgc ggcggcaaaa atacgaggag aaaagacaga cggcagttgt gggaagtgtc
     1981 ctggaaccaa cgctggtctg gctgggcgta gttgggtagc cacgccatca tgatgcaaca
     2041 gacgggccaa agcaatatta cagggcagag aaaaatggag gaattcctga tgaatatgtt
     2101 tcaggccggc aacgacttca tgattcgccg cgggcaatgc agcacatcaa cgtgtgacct
     2161 gaaagcgcca ttttgctcca gcagaacgta ttgagagaaa agtctcctgt gaaatgggag
     2221 gaaagttcta agcttataag gagagatgga agcacttact tgcatataat cttccgtaat
     2281 aatcaacatt aaagtttcat ctatccggaa tccatgatgg tattgcactg tttttaaaaa
     2341 ttaatttaga ttgaattagt tcccctttta cggacttaaa cacatataaa aggacagaga
     2401 aatgtcacgg gaagaaatta ttgcagctat agacgaagaa ataagccgtc ttgagaaagt
     2461 tcgtgcgttg ttagaaggcg ctggcacaac ccggattttt gccggggcgt ttgccaacgg
     2521 ccgtaaaaaa cgccatctga gcgctgaagc acgcaggcgc attgctgaag cacagaagcg
     2581 ccgctgggcc aagcagaaga aacagcctgt ttccagccaa taattcctgg cgggacagct
     2641 tatgattgca gtagatgatc aaagtaaagc tccttcatcc tgacgcccgc gtgcctacgg
     2701 tgaattatcc cggaagcgat ttaggttatg acctctattc aattgaggac ataactcttc
     2761 acccaggggt gcccgtaaaa gtccggaccg ggatagcttt ggaagcggcc gatggatatg
     2821 gttttgtcct cggcgaccgc tcttctatgg ctgcgaaggg tataacttat gccggcggaa
     2881 ggattgacgc gggttatcgc ggggaaattc ttgtctgcct gatcaatgta aaccaaccgc
     2941 gttactccct gcgtgtgacc cacgacgcgc agggaaggcc cactgatgta gatctgcaac
     3001 aaacggacgt aagtgtcacc atccgtaaag gcgacaaaat cgcgcagatg tccccgattc
     3061 tgcctgcaac ccaccttcct gtgcttgcag tagaagaact ggctgagagc agccgtgggg
     3121 ccaaagggtt tggctccagc gggcgttaac aacgtggatc ttcgccctct tttaccatgt
     3181 attcatggaa acagggctga aagaccgtgt tgctattgtt gctgcttcga gtcagggaat
     3241 tggaaaagcg gctgcgctgg cttttgctgc tgaaggcgcg catcttgctt tgtgcgcaag
     3301 gcagccgggg cccctggatt ccgtggcacg tgaagccacg gaccgctatg gcgtgaaggt
     3361 cttcacggaa gccttcgacg tcagcgatga tgccgctgtt cgtagcttcg taaagtctgc
     3421 acacgaccat tttggtcacg ttgacgtctg cgtaacgaat gcaggcggcc ctccggcaaa
     3481 accgttttta gcaacgaccc tggacgaatg ggagcgggca tggcaattaa acctgcgtag
     3541 catcgtttcc tttgcacagg ccgtcattcc atacatgcag aaaaagaaat ggggccgcat
     3601 tgttacgctt acctcttata cagtgaagca gccggtgccc gaacttgtgc tgtccaattc
     3661 cattcgcgca ggagtgatgg gccttgtgcg ttcgcttgcc ggccagtttg gcccagacgg
     3721 cattaccgtg aacaatgttg gtccaggatt cacctccact gcacgagccg aggagctgct
     3781 ggagaaacgc gcggctgccc gcaacacgaa catcgagcaa atcaggcagg aactgcttca
     3841 ggacattccc gttcgccgca tggcgacacc ggaagaggta gctgccgcta tcgtctggct
     3901 ggcttcagag caggcatcct gcattacagg acagacactt ttggtggacg gtggcgcata
     3961 tcgcgggctg tagccggttg ccaaaccgac gagtggtgaa aagtcgcgca atgaaaaact
     4021 tttctttttt gcatttttgc cgcgacatct gcataaacac tggtgttctt ctgaatcgaa
     4081 acgggcgccc ggatacagaa gagatcagga gcattttatg aaacccacga gaaccacggt
     4141 tctgagcatg gcaatctttg cgttaagtac gacgctgggg ctggcccaga cggcttccac
     4201 acaaacccag acgcagaccg acagcaaaat gactgtcaat cagcgccgtg aaaaccaaca
     4261 ggaccgtata gcgaacggcg tgcagagtgg acaacttacc gccgctgaaa cgaagaacct
     4321 tgagagcagg gaagcaaacc tgaaccgcga aatcaaagac gaccgaaaag cggatgacgg
     4381 ccatctgacg accgcagaga aacagcagat caatcatcag caaaacaacc tgagccgttc
     4441 cctctatgat gacaagcaca atgccaacac tgcgcatttt ggaaatgggg aagttgggca
     4501 gcgccgcgaa aaccagcagg accgtattgc gcaaggtatt cgcagcggcc agttaacagc
     4561 cggggaaaca tcccgtcttg aaaaccgcga gcaggggatc aatcagcaaa tacacgccga
     4621 ccgccaggca aacggcggca aactgacggc tggggaaaaa gcacagatga atcgccagca
     4681 gaatcgtgca tcacgcgcca tctatcgtaa taagcacaat ggcgcgcatg gcccacgcta
     4741 gttgtcctcc cgcgcagcca gcgtgccatg cagagaaagg gccttcattc ccacgagtga
     4801 aggtcctttc ctttcggtga atcgaaatct tctcagtcgt ttttggttat caaatcaatt
     4861 aacaaattat gaaataaatg ttaattgcat tagaccaccc cagccctctg aggctcatcc
     4921 taagcatccg aagtaagttg tgtttcgaga tttctttgtt tgctatggag gccctatgca
     4981 acaaaagtct gacgcgattt cccgtccttt ttttgtttca gtcctaagag catctctcag
     5041 gaaacacgtg cgcggaattt tgatattgcc gctcgtgctc ttgtgcacag tggcgctgca
     5101 tgcgcagttt cgcggatcgc tccgcggcac agtgactgat ccgcagggtg cgattattcc
     5161 cggagcaacc gtcacgctta ccaatacaga tacgaaccag agccagaccg cgacgacgga
     5221 caacaatggt atctatatct tcaatggatt acctccggcg catttcaggc taacggtgga
     5281 tgcatcagga tttaagcaaa aagtgctgga acacgtccag atcattcctg agcagttgaa
     5341 ctcgttaaac gttcagcttg aagtgggcgg agcgcagcag accgtgactg tttccggcac
     5401 tacgcaggcg ctggactctg aaacagcaac ggtaagcgcg acggtgagca gcaaccagat
     5461 acagcatctg ccatcattta accgtgacgt atttcaactt gcgcaactca cccctggagt
     5521 gtttggtgat gggtcgcagg ggggcggcgg ggggacattt aatctaccgg ggaaccaggg
     5581 accgggtggc agtgggggtg gtcaggcggg aatttttgca actgaaaatg gccctcaaat
     5641 tcaaacacgt ggtggacaat acgagaccaa cggtatttcc attgatggca tcagcacggt
     5701 gagtgcagtc tggggcggca catccgtcat cacgcccagt gaagattctg tagacagtat
     5761 gaaagttgta tcgaacagtt acgatgctga aaatggacgc ttcagcgggg cgcaaataca
     5821 ggtcacatcg aaaagcggca gcaacgagtt gcatgggagc gcattcttca agatgtcgcg
     5881 tccgggattg aatgcttacc aacggtggaa tggtgtgagt tccaattttg caggcacggc
     5941 tgcggaccgc ggggtgaacc gtgataacga ccggttcaat aattatggtg gaagcctggg
     6001 tggacccatc tggagagaca aggtatttgc ctttttcaac tgggagacaa gtccgctctc
     6061 agcagtcaat actgcacaag ggtggtatga aacatctcag tttgattccc ttaccggaat
     6121 tggtccgatt gcttggaaat acttgagtta tcccggagaa gctgtttcgg caaccggtat
     6181 tttacaaagg gcatgtgctt cgattggcct gcaggaaggt gtgaattgcc ggacaacttc
     6241 ccaagggttg gatgtcggtt cacctatgaa gaatggtatt ggtcttcagg acccaactta
     6301 tggtggcaac tccaaccagc ccggagttgg aggtggtctt gatggcatag cagaccttgc
     6361 ttattttaac tcgattagtc cctcaaaaac ctctcaaaat cagtacaatg gccgtctgga
     6421 tgtgaacgtt acacaaaatg accatctggc ttttgccatt tactgggtgc ctgttgattc
     6481 aaccttctat aacggtcctg tgaggtcagc aaatttatgg caccactcac agatcaatga
     6541 tgcattttca ctcatctgga accacacttt ttctcccact ttgctgaacc aggcacgcgc
     6601 caatgcagcc ggatggcgct ggaatgaaat caactcgaat cctcaggaac cattcggact
     6661 accgcaggca aatattgacg caattggaac gatcggcaac cagggaggct ttcagttctt
     6721 cggtgcgccc gggccaagca attttaacca gtggacttac agttacaatg acgtgcttac
     6781 gaaggtcctg ggaaggcaca acattaaggc aggtggtgaa ttgacccggc tatattacct
     6841 gaacaacccg gtgtatgctg cgcgtcctgc cttcaatttc aacaatctct ggacattcgc
     6901 aaatgatgct ccatattttg agagcggaca atttgactca aagaccggaa ttccttttgg
     6961 caaccgccag gacaaccggg aaaatatctg ggggggtttc gtacaagatg actataagat
     7021 caagccaaat ctcacaatca atctcgggct gcgctggtct tatttcgggg ccttctattc
     7081 caaggaaaat aatttagggg tgctgcgctt tggcagtgga ccaaatccgc tggatggcct
     7141 gaacatcaga atcggcggag gtatctactc gccgcaaaaa aacaattggg gccctcaggt
     7201 tggctttgcc tggcagccga agcaagccaa tggaaatctc gtcctccgcg gcggattcgg
     7261 catcaactac aaccaaaacg aaattgccat tacggccaat ggaaatggca atccgccttt
     7321 tgtggtgcag gcaaacttca cctgtagtta tccatacaca aacaacccat cctgcgctgg
     7381 gaccggaatt gtgtatcaga cagccaccga tgtgcattct ttgtttggtt atccgccgaa
     7441 tcccaatgcg gttaaaactt ttggtcccga taatctgcct cttgccggat cagcgcccat
     7501 ttttgtgacg ggctttctgc ccaacccaaa gaccattgcc aactatcact actctttgga
     7561 tatgcagtat cagttaccgc tgggcctcgt ggcttcattg gggtatcagg ggagccagat
     7621 gcgtcatctg ctgatccaga acaactggaa tgcaattgca gcagcaagag gactgtcgtt
     7681 aaatccaaaa gtcaatttcc tcgactttta tcagaacagc ggaaccggta actacaacgc
     7741 gatgatagca acactggatc acagttttag tcatggtttt caagcggaag tcatgtatac
     7801 gtgggccaag gcgatggacg aaaactccgg cccctactct gaagattact atccttatga
     7861 tacccatgcg gcgtatgggc gttctgatta caacgtggcc aatgctgtaa aaattttcgg
     7921 tttatggcag ccaatcttct tccacggaag tcatagctgg atcgagaagg ttgcaggtgg
     7981 atggtcactt agcggaatct ggaactggca tacaggtttt ccatggaacc ccatttataa
     8041 cgctgcggga agcctgtact atccaggcag cggttatgga cagcttcgtc cggcagccag
     8101 gttgcgcggc tatgggacaa atacgagcaa caagacattt caacaggata cgaatccaaa
     8161 ctttggagga gacggcacca aattcttccc gggaccgacc tatgttgcgg caacaggatc
     8221 gtccttcaca gatacagtgc ctgcaccggt agccggcatt cagcgaaaca gcctgaacgg
     8281 cccaaattat aacgacgtag acgccagcct ggccaaagct tttgggcttc caggtatgcc
     8341 gattctggga gaaaacgcta agtttgagat tcgagcagac gcgtacaatc ttttcaataa
     8401 gctgaatatt aacccctcat ccattgacaa caacctcggc tcggtcgctc ccaatggaac
     8461 ggtaacgccg aacccggatt tcggggtggc cggctcagca ttgggcagta gaacagtaca
     8521 aatacaggcc cggttcagct tctagccaga gtgatctgtg tggggtgcgc ccagtgacgc
     8581 accccgatgt ttctcctttt gtattgctcg ttgtccggag gacctcgctt gaaatttctg
     8641 catgcagtcg ctgtctgtgc gacctacatt ttcctcctgc tagcgcaggc ctttgcgcag
     8701 gagcaggtca atattaacgc tcaggcgcag accattccat taccgcattt ctgggaggag
     8761 atgtttggct caggccgcgc aaaccttacg ttgcgcgaga gctatcgtaa cgacctgcgg
     8821 gcggtgaaaa aaatcacaga cttccactat gtgcgttttc acgcaattct gcatgacgag
     8881 aacggcgtct acaacgaaga tgagcatgga aatccggtct ataacttcca gtatgtagac
     8941 cagatctacg atgggctgct aaagaacagc gtgcgtcctg tcgtcgaaat cagcttcatg
     9001 ccgaagaagc tcgcgttcaa tcccgacgca ctgcatccgt tttggtacaa gcagaatgtt
     9061 tctccgccaa agagcatgga gcgttgggac gacctaatcc ggcatctggc gcaacatctg
     9121 gtggaccgtt acggcattga tgaagtctcg caatggtact tcgaggtgtg gaacgagccg
     9181 aacattgatt tctggaacgg cattccgcgc aagcagtctt actttgaact ctacgcgcat
     9241 acggcgcgcg atctgaagtc ggtgagtccg cgtctgcgcg taggcggacc tgcaactgcc
     9301 gctgctgcgt ggatccctga ttttctgaaa tacacggcag aaaaccatgt tccggtggac
     9361 tttgtttcaa cccatggcta tgcggatgat acggtcgaga accttttcgg cacgcatgaa
     9421 aatattccga tggatgatcg agtatgccgc gcggtcgcca aagtaaaagg cgagatcaag
     9481 gcatcggcca tgccaggctt gccgctgctg tggacagagt ggaacgttcc cggagagatg
     9541 gaagcgcgcg acaccatcta cgttggccct gcgctggcga acacaatccg tgaatgcgcg
     9601 ggtgaaggcg tcgaggcact ctcgttctgg accttctccg atgtttttga agagggcggc
     9661 ccgattcctg ctccgtttac tgggcagttt ggattgcgtg cgcaaggcgg catcaacaag
     9721 cccagctttt atgcctttgc actgctgcat cagcttggga accagttgct gccgaatgcg
     9781 tcgaagaacg tgattgtgac aaaaacaccc gaaggcggcc tggccattgc cgcatggaac
     9841 cttgtagacc cgggcgagca cggtgcaaca aagcaaatta cgcttgtgtt tcagggattg
     9901 cgcgaggatg ctccggtttc aatcaatcgc gtggatgaaa accacgggaa tgtcctgaag
     9961 gcatatgcgg caatgggcag gccagtgtat cccactccgg agcaggttga gcagctaaac
    10021 cgcgagacag acctgggtgt gccggagcag cgtcatctga atcatggcaa gctgacagtg
    10081 gatttaactc cgaacgcgtt gttgctgatt aaaatcgaaa aataagtaga aaaggagcag
    10141 cctttgttct ccgcacttgt ttgcagagta ggtgtacttg gattgagcgc gagtgtctac
    10201 gcaggtgctg tcctcgcgca aagtgcgcga ccttccaacg cgcagcttgc ctcccctcag
    10261 atcgagcgca aagtagatga tctgctgaag aggatgatgc tcgacgaaaa gctgggtcaa
    10321 ctggtgcaat acaacgatgc tggatacatt gcagcaaccg ctgcgcaact tgcgatcaat
    10381 cccgaggcgc gcaacaaagt ggacgccatg cagcttgcgg ctgaagggaa gctcggctcc
    10441 atgctcaaca ctgttggcgc agagcacaca aatgcttttc agcgcgcagc cgtagagaag
    10501 agccgtctgc atattccgct gctttttggc gcggacatca ttcatggctt tcgtacgatt
    10561 tatccggagc cgcttgggct tgccgctacg tttgatcctg aactggtaag ggaactgtcg
    10621 cgcatgtccg cggaagaagc gaccacggcc ggtattcgct ggttctattc gccaatggtg
    10681 gacatctcac gcgacgcgcg ctggggccgc acggcagaag gtgcaggtga agatccatat
    10741 cttggcgcgg cgatggctcg tgcctacgtg cgcggctacc agcaggacga tctttccaaa
    10801 ccagacagcg tcgccgcctg tgtaaagcac tttgccgcgt acggtgcggc cgaagcaggt
    10861 agggagtaca acacgacgga catgtcggac atccgcctgc ggcaggtgta tttgcctccg
    10921 tataaggctg ccgtggaagc aggggcggct acgatcatga gcgcatttaa cgcgctgaac
    10981 ggcgtgcctg caacggcgaa cccttatctg cttcaaaaaa ttctgcgtga tgagtgggga
    11041 ttcaacggct tcgttgtcag cgactacaca gcagtgatgg aactgcaaaa ccacggcatt
    11101 gcgctgacac cggcagaggc agcgaaaaaa gcatttctgg caggtgtgga tgtagacatg
    11161 atgtctcatt actacgacac gctgcttcct gagttgattc gcagtgggca ggtcccgatg
    11221 aacgcggtgg atgaggcggt gcgccgcgtg ctgcgtgtta agtttgcgct cggactgttt
    11281 gagcatccgt atgcgcgtgg gacagaagtg acagccgcag tgcctgagca tcgtgcactg
    11341 gtccgtcgcg cggcagaaga gtcgtttgta ctgttaaaga acgacgcgaa catattgccg
    11401 cttgtgcaga aggcgcagaa gattgcattg gttggtccgc tggcggacaa tccggctgac
    11461 atgctcggtg cctggtcagg cggcccgcaa atgaaagatg tagtgacaat ccgtgctgct
    11521 ctcgaagccc gggcaaaaca gtatggcggg caggtgttgt atgcaaaagg cacagatatc
    11581 cagggcacat ctgagtctgg cttcgcacag gctgttcagg cagcaaaaca ggctgatgtt
    11641 gtagtaatgg cattaggtga atccggcaca atgagcggag aagccggatc gcgcgcacat
    11701 ttggatttgc ccggaaatca ggaacagctt cttgaaacaa ttgctcaaac cggacggccc
    11761 gttgtgctgc ttgtcttctc ggggcgtcct ctcgtgctta cgtgggcagc cctgcatatt
    11821 ccggccatca tggaagtgtg gttcccggga acagaagcag gtaacgccat cgccaatgtg
    11881 ctgtatggcg atgtatcacc gagtggcaaa ctcccgatga gttttccctg gactgtggga
    11941 caggagcctc tttactataa ccagtttccg accgggcgtc cgcctgcccc ggaaatggat
    12001 ctgacgcgac ctccggggcc agattcgcgt tttgtgtccc ggtatattga tgctcccaat
    12061 gctgcgctct ttccgttcgg atttggtctg acctatacca gcttttctta tggaaaggtg
    12121 tcgctctcgc ggaacgctgt tcctttgaac gaagccaatc atgaggacgc gaaaaaattg
    12181 gtcattgcaa cagccacggt tacaaataca gggacccggg aggccacgga agtagtccaa
    12241 tgctacgtgc gcaatctggg agcgagtatt gagcagccag tgcgtagcct gaaaggtttt
    12301 gcgcgtgtca cactcaagcc gggtgaatcg aagagggtgt cattcgatct ggggtttcct
    12361 gagctttcgt tttataacag cgagggaaag caggtgatcg aaccgacgca ttacactgtc
    12421 tggattggcg gaagctcact ggccaaagag gaagccagct tcgatgtgaa gcagtaaccg
    12481 tcagtaaatc ttcggttcgc cgtcgggacg ggtcttccat ctacggtgga cccagagcca
    12541 ctggtcggga taactccgaa tgcaggcttc aatagcggca gtacagcggg ccgtgttgac
    12601 gatggtgtcg gcttcatcgt ctcctgtgct gaccatctgg atttcttcac caaagtgcaa
    12661 cacgtattcc tgcctggatt cttcccagag catgaatccg ggcagcaccg ccgcacccgt
    12721 cttcagcgcc acgcgcgcaa gaccggaagc agtgcaggcc agcattccaa aaaacggaac
    12781 gaagacccct tgcggcggag tcatgttcgt gtccatcagg atgccgacgg tctcaccttt
    12841 gcgcatggca gcaagcaggc cgcgtgcaaa atcgtcctta tgcagaacgc ggttgccatg
    12901 caggcagcgg atgctgttta caaggtggtc tactttggga ttatcgagcc ggcggatgac
    12961 catgctcatg ggatggccca tgagtgagtg ataaaagctt gacaactccc acgcgccgag
    13021 gtgtccggtg acaatgagca cacctttgcc tttatcacgc gcggcaagat aacggtccag
    13081 accttcataa cgaataaagt ttcgcgtgtt ctctggcgtg taataggaca tctgacagaa
    13141 ctcagccagc aaccagccca ggttccgata gagtttgcgc agaatctgtt gccgctctgc
    13201 gctgctcttt tccggaaaag ccagttcgag gtttttcaag ccgacttttc tgagacgagg
    13261 aactgtgtga aaggccactg ctcccagcgc cgctccgatg aagcgagcaa ggctgcgtgg
    13321 aagcgcgcca agaatgcgca ccagcagcca gacgatggcg aattcaaggc tttcacgcat
    13381 gaataatgga gtaagtgtca gtgtaaatca gcaaaggacc tgcggcattg cgatgaagaa
    13441 gcaagtcctt ctccgtctt
//
LOCUS       DASRBV010000160         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_567745, whole genome shotgun sequence.
ACCESSION   DASRBV010000160 DASRBV010000000
VERSION     DASRBV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567745"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFA02_06140"
     CDS             <1..219
                     /locus_tag="VFA02_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09467.1"
                     /translation="DANHWWMAMLGDVPCFEEGQPAQRFTLLPWNIAESRNPEMYHLC
                     GQGCVMQAVERFMSSGKIEPEMSAHARG"
     gene            complement(226..708)
                     /locus_tag="VFA02_06145"
     CDS             complement(226..708)
                     /locus_tag="VFA02_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZU09468.1"
                     /translation="MQQRATRKKGPATAVIPDKLYFRIGEVARLCRVETYVLRFWESE
                     FPQLKPNKSGTGQRLYRKRDVELALKIKRLLHEEGYTIAGARHVLQSENREGKKQNTQ
                     PELPLNKSSDKTQARLQHLRAELKELLGMLSSGRPAPVRHQPKARKGHMHSAPNLFGE
                     "
     gene            complement(913..1104)
                     /locus_tag="VFA02_06150"
     CDS             complement(913..1104)
                     /locus_tag="VFA02_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09469.1"
                     /translation="MMHPQQPSTTYRDAPQPQPEADGQQPPQHHSVYCPNCSSHLTGH
                     RCKLICNRCGYYLSCADYY"
     gene            1318..2379
                     /gene="thiL"
                     /locus_tag="VFA02_06155"
     CDS             1318..2379
                     /gene="thiL"
                     /locus_tag="VFA02_06155"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZU09470.1"
                     /translation="MRGKANKALVSGERAFISAIRRRAGRSGIVRTGIGDDCAVLRPP
                     RGSEILVTTDFSLEQVHFRLDWHPPESAGHRCLARGLSDLAAMGAKPVAAFLSLALPA
                     ELTSDWAKRFLDGLLALADEYGVPLAGGDTAQSPAFDESGKRQTGMVLADMVLIGAAP
                     QGKALLRSGARAGDIIYVTGALGGAAAELLALGREPKAFRRLTKAASGHPHLYPVPRL
                     EVGARLRGMAGAAIDLSDGLSTDLAHVCEESGLSAEINEAAVPIHPLASKAEADGWVP
                     SALHLALNGGEDYELLFTVPAGRRVPGKMAGIPIRAIGRIKPRTRGKSLVEMVTATGK
                     RVPLHAGGWEHFRPKTGQH"
     gene            complement(2454..2942)
                     /gene="folK"
                     /locus_tag="VFA02_06160"
     CDS             complement(2454..2942)
                     /gene="folK"
                     /locus_tag="VFA02_06160"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZU09471.1"
                     /translation="MTAYIGIGSNLASRAGSPAQIVQAAMSALNGLGKVVARSSLYET
                     EPVGFTDQPAFVNAVAALETELSPEDLLEKLLDIERRFGRDRKSSVPKGPRTLDLDLL
                     LADNLVINTPTLTLPHPALAARRFVLAPLAEIAPGLRHPLLQRTMRELLQSLPEKGVR
                     LL"
     gene            2973..3644
                     /gene="nadD"
                     /locus_tag="VFA02_06165"
     CDS             2973..3644
                     /gene="nadD"
                     /locus_tag="VFA02_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HZU09472.1"
                     /translation="MRIAFFGGTFDPPHCGHLRIAQAAADRLHLDKVLFAPVGQQPLK
                     HDPSSASFQDRMAMVELAIASFPRFEVTALDAPRADGRPNYTIDTLVRLRESVDAQDR
                     LFCLLGADSFLTLRHWYRASDILFVCGFIVAGRPGAMLGELSAALPKGVSAEGDAPES
                     AEVVRMKLRGSEGRHSELFLLPDLHEDVSATQIRAALAGGRQAAQVLPEDVAAYIREH
                     GLYRK"
     gene            3712..>4046
                     /gene="rsfS"
                     /locus_tag="VFA02_06170"
     CDS             3712..>4046
                     /gene="rsfS"
                     /locus_tag="VFA02_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZU09473.1"
                     /translation="MASTETRQMVLAAASACEDKKAENTRILELDAADSGFTDFFLIT
                     SAANDRQAVAISDEIELRLKRDFHTYPNSVEGRRMGEWILMDYVDFVVHIFLHEKRAF
                     YDLERLWKSA"
BASE COUNT          740 a         1109 c         1334 g          863 t
ORIGIN      
        1 gacgccaatc actggtggat ggcgatgctg ggagatgtcc catgttttga ggaaggccag
       61 cctgctcaac ggtttacgct gctcccctgg aacattgcgg aaagccgcaa tccagagatg
      121 tatcacctct gcggacaagg ctgcgtgatg caggcggtgg agcggttcat gtcctctgga
      181 aaaatcgagc cggaaatgtc ggcccacgcg aggggttgaa ctgaactatt ctccaaacag
      241 attcggggcg gagtgcatat gccctttccg ggcctttggc tgatgacgca cgggggccgg
      301 ccttcctgaa gaaagcattc ctaacagttc tttcagttcc gcgcgaaggt gttgcagccg
      361 tgcctgggtc ttatcgctgc ttttgttcag aggaagttcc ggctgagtgt tttgcttttt
      421 cccttcgcgg ttttccgatt gcagcacatg gcgcgcgcct gcgatcgtgt agccctcttc
      481 atgcaaaagg cgcttgatct tgagcgcaag ctctacatca cgcttgcggt agagacgctg
      541 ccctgtgccg cttttgttgg gcttgagttg cgggaactcc gattcccaga agcgcagcac
      601 ataggtctca acgcggcaca gcctggctac ctcgccgatg cgaaagtaca gcttatccgg
      661 aatgactgcc gtggccggcc cttttttgcg cgtggcgcgt tgctgcataa ctactgccgt
      721 cctgaaaagt ataggccaca ggacagcatt cttagcaagt caggaaattt gtcccgactc
      781 agcgtgatta gctataagac gccgcggccg tctcaagcgt gatgtcctgc gccatgtgga
      841 gagcgcaagc gttgtaagtc cacgcaggcc gtctggtttt cctatgagtc cggaaccggt
      901 tgatggcacg tgtcagtaat aatcggcgca gctcagataa tagccacatc ggttgcagat
      961 gagtttgcag cgatgtccgg tcagatgcga ggagcaattc gggcagtaca cggagtggtg
     1021 ctgcggaggc tgctgcccgt ccgcttccgg ctgcggttgc ggcgcgtcgc ggtatgttgt
     1081 ggatggctgc tgcggatgca tcatgaaaaa gattagccga gaatttacct ctgcggtagt
     1141 gtgcagcatc tcaggtctca tatccacgat attgtgtcac ggcgcttctc cggtctcggc
     1201 ctccgcggcc tcgcgcacat acacgttctt ccagtccgtg tacaaagcgc gtgggctatt
     1261 ggggtccgac atcgggattg attgtttccc cagctttttt ctcagtatgc ttgttctgtg
     1321 cgcggaaagg cgaacaaggc gctggtttcg ggcgagcggg ccttcatctc cgccattcgc
     1381 aggcgcgcgg ggcgctccgg catagtgcga acgggcattg gcgatgattg cgctgtgctg
     1441 cggcctccgc ggggaagcga gattcttgtc acgacagatt tttcgctgga acaggtccat
     1501 ttccggctcg actggcatcc gccggaatcg gccgggcaca gatgtcttgc gcgtgggttg
     1561 agcgaccttg cggcgatggg ggcaaagccg gttgcggcgt ttctgtcgct ggccctaccg
     1621 gcagagctta catcggactg ggccaagcgc tttctggatg ggctgcttgc tctggccgat
     1681 gagtatggcg tcccgctggc cggaggcgat acggcgcagt ctcctgcctt tgatgaaagc
     1741 ggaaagcgcc agacgggcat ggtgctggca gacatggttc tgattggagc tgcgccgcag
     1801 gggaaggcac ttctgcgctc cggcgcaagg gccggagaca tcatctatgt taccggagcg
     1861 ttgggcgggg ctgcggcgga actgctggcg ctggggcgtg agccgaaggc gttccgcagg
     1921 ctgacgaagg cggcctctgg tcatccgcat ctttatcctg tgccgcggct ggaggtgggt
     1981 gcgcggctcc ggggaatggc aggcgctgcc atagatctga gcgacgggct gtctactgat
     2041 ctggcccatg tgtgtgagga atcgggcctg tcggcggaaa tcaacgaagc agcggtgccc
     2101 attcatccgc tggcaagcaa ggcagaggcg gatggatggg tgccttcggc gctgcatctg
     2161 gcgctgaatg gcggcgagga ctatgagctg ctcttcactg ttccggcggg cagacgagtg
     2221 ccggggaaga tggcgggcat cccgattcgc gcgattgggc gaatcaaacc gcgcaccagg
     2281 ggaaagtctc ttgtggagat ggtgacggcg accggaaagc gggtgccgct ccatgcagga
     2341 gggtgggagc atttccggcc gaagaccggc caacattgac tcaacgactc aacacaggca
     2401 ctgttccctc aggtctcttc gagacctgtg cattcgtcct ttttacgggt tcactacagc
     2461 agccgcactc ctttttccgg cagggactgc aggagttcgc gcatagtcct ttgcagcagt
     2521 ggatgccgca ggccgggggc gatttctgcc agaggcgcca ggacgaaccg ccgggctgca
     2581 agcgccgggt gtggcagcgt gagggttggc gtgttgatga cgagattgtc cgccagcagc
     2641 agatcgaggt cgagcgtgcg cgggcctttc gggacgctgc ttttgcgatc gcgtccaaac
     2701 cggcgctcga tgtccagcaa tttttccagc agatcctctg gcgatagttc tgtttccaga
     2761 gcggccaccg cattgacgaa ggcaggctgg tcagtgaatc ccaccggctc ggtttcatac
     2821 aacgacgagc gggccaccac tttgcccagt ccgttcagcg cagacattgc ggcctggacg
     2881 atttgcgccg gagaccccgc gcgcgatgcc aggttcgagc cgatgccgat gtacgcggtc
     2941 ataatgacag actacgttac gatcacaaga ggatgagaat tgcgtttttt ggcggcacgt
     3001 tcgatccgcc gcactgcggg catctgcgca ttgcccaggc cgccgctgac cggctgcatc
     3061 tggacaaggt gctgttcgcg ccggtggggc agcaaccact caagcatgac ccatccagcg
     3121 ccagcttcca ggaccgcatg gcgatggtcg agctggccat tgcgtctttt ccgcgctttg
     3181 aagtgacggc gctcgatgct ccgcgggcgg atgggcgacc gaattacacc atagatacgc
     3241 tggtgcgcct gagagagtcg gtggatgcgc aggacaggct tttctgcctg ttgggagcgg
     3301 actcgtttct tacgctgcgg cactggtatc gggcgtccga catacttttt gtgtgcggat
     3361 tcatcgttgc gggccggcct ggagcgatgc tgggtgaact cagcgcggcg ctgccaaaag
     3421 gagtcagcgc agaaggcgat gctcccgaat cggcagaggt ggtgcggatg aagctgcgag
     3481 gcagcgaagg gcggcattcg gagctttttc tgttgcctga cctgcacgaa gatgtttcgg
     3541 ccacgcagat acgcgcggcg ctggcaggag gaaggcaggc cgcgcaggtg cttccggaag
     3601 acgtggccgc gtatatcagg gagcacggcc tgtatcgaaa gtaggcaggg cggcgctggc
     3661 gtttcccgcg gctccacgct aggatgaagc gttgggagaa cccactaccg aatggcatcc
     3721 acagaaaccc gtcagatggt gctggccgcc gcctcggcct gcgaagacaa gaaggctgaa
     3781 aacacgcgca tccttgaact ggacgcggcg gactccggct ttactgattt ctttctgatc
     3841 acgagcgcgg ccaatgaccg ccaggcggtg gccatcagcg acgagattga actgcgcctg
     3901 aagcgcgact tccacacata tccgaattcg gtggaaggcc ggcggatggg cgagtggatc
     3961 ctgatggact atgtggactt cgtcgtccat atttttctgc atgagaagcg cgcgttctat
     4021 gaccttgagc ggctgtggaa gtcggc
//
LOCUS       DASRBV010000161        18249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_415210, whole genome shotgun sequence.
ACCESSION   DASRBV010000161 DASRBV010000000
VERSION     DASRBV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415210"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFA02_06175"
     CDS             complement(<1..377)
                     /locus_tag="VFA02_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HZU09474.1"
                     /translation="MMRHFWKIALCLAFCSPVFSAPGQKLVWSDEFNNSTTPSAPDPH
                     NWTYETGAGGWGNHELEDYCAYASQRPPCDSSRPNVFVWKGHLHIVARRDEHGHYTSA
                     RLKSQGLRSFQYGRIEARIKIPKG"
     gene            425..835
                     /locus_tag="VFA02_06180"
     CDS             425..835
                     /locus_tag="VFA02_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HZU09475.1"
                     /translation="MLVNFPPRDSVSEEQIATLVDTFYTRVRQDEVLGPVFEHVIGDE
                     WVPHLEKMRAFWSSLVLASGRYKGNPMMAHLMIAPRIGAEHFQRWLNLWRQTTAEIFP
                     QQIAAIFVRKAESMAERLLETIDQYHASLAFSQN"
     gene            complement(822..1706)
                     /locus_tag="VFA02_06185"
     CDS             complement(822..1706)
                     /locus_tag="VFA02_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09476.1"
                     /translation="MDILSPTVFHVCNFMAGYRAIRLFLQSGRFLLALPMIAYGIVHW
                     RYAHFITSFVPPWVPCHLFWIYFTGTALIAAGLAIVARKVAWLAGVLLGAMVLAFVVL
                     IHTRLLLSLPGDAYAHQKIFDFPLYGRISNACKDLALSGAAFLFAGTQVSGRNRALTL
                     GRILFALPVIGFGILHFASTVYAPGIPPMLPSVHSPLPWQPFWDYLTGGIFLITGVWI
                     LLGRNLFFAGTMLGATILVFGLIVWGPPLAAHPGNILTSNFLKDVGVAGGAWCLAASV
                     CCKLAEAPVLVEKTVSSG"
     gene            1861..2862
                     /gene="hpnA"
                     /locus_tag="VFA02_06190"
     CDS             1861..2862
                     /gene="hpnA"
                     /locus_tag="VFA02_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZU09477.1"
                     /translation="MKAFVTGSTGFVGSHIAKALAKEGAHLHLLVRRNSNMANLSGMH
                     APPSFSTTTGDLLEPESLRPGIRGCDYVFHVAADYRLWVRDPKQMYAANVDGTRELLR
                     ICREEGVKRVIYTSSVATMGFRKDGTIVDENTPVSLNDMVGHYKRSKFLAEQEALKAA
                     YDGQEVMVLNPTTPIGPGDIKPTPTGRIIVDFLKRKFPAYMDTGLNLVDVDEVARAHI
                     AAMTRGTPGERYILGGENLTLKQILDKMSAITGLPSPSVKVPHTVALIFAFFDETING
                     RLLGKEPRATVEAVRMGRKKMFASSAKAVRELGFKQVPVYHALRAAIDWFRANGYAP"
     gene            2859..3515
                     /locus_tag="VFA02_06195"
     CDS             2859..3515
                     /locus_tag="VFA02_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HZU09478.1"
                     /translation="MKRIGIVAALHGELKPLVRGWEKRGQLWIGRTGETHCMATAGGM
                     GARAAARACEVLLKEGPLDALISVGWVGALSCGLKPPQAHVISEVIDARTGERFVTAS
                     GEGQRLLTTGHVVRPDEKRKLAQQFQTPLVDMEAAAVARIAREQQLPFYCFKAISDGY
                     TDRLPDFNPFIGPEGQMRMANFVAYAMLHPQYWGALMRLGRNSSIAARNLAALIMRQL
                     "
     gene            3512..3727
                     /locus_tag="VFA02_06200"
     CDS             3512..3727
                     /locus_tag="VFA02_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09479.1"
                     /translation="MKQEDEITFRRKRQGCEGHSEIGLPGLDVERRAGQRWQKIPAVV
                     YTCDSVMMPATTMSEMEMSISTATMAV"
     gene            complement(3983..4420)
                     /locus_tag="VFA02_06205"
     CDS             complement(3983..4420)
                     /locus_tag="VFA02_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU09480.1"
                     /translation="MSVQEGLLSPRISWGILLVRVLVGWVFLSEGIQKFLFPAALGAG
                     RFLKIGIPMPQFSGPFVGVVEIVCGTLLIVGFFTRWASLPLLIDIAVAIATTKLPMML
                     KQGFWAAMHDGRTDFCMFVGLISLLFFGSGRFSLDEKLGLRSK"
     gene            complement(4446..5933)
                     /locus_tag="VFA02_06210"
     CDS             complement(4446..5933)
                     /locus_tag="VFA02_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZU09481.1"
                     /translation="MQSSSTTRRNFLKLAGAAAALIPSLQLDAQASIQAPEQAGQKAE
                     YTLHIQNGLIEIGKNRYVSTTTFNGQFPGPLLRFREGEQTTVEIHNDTDIPEQLHWHG
                     QNVPVNVDGAAEEGTPFVPAHGMRRISFVPQPSGLRFYHTHLRAGGDLNRGQYTGQVG
                     PVYIEPKQNPGHYDREVFLTLKEFEPWFSHGGDMANDFLAGQEVKELKDRGESEMATS
                     VKEGKPHGFEVNYRTFTINGRMLGHGEPIRVKQGERVLFHVLNGSATEIRSLALPGHA
                     FSVVAMDGNPVPKPAQVPVLWLGTAERISAIVEMNHPGVWILGDMDDDDRHHGMGIVV
                     EYAGQQGKPQWIAPKQVHWNYLHFANSAEQTAQPDDVFEMTFAKRNAALDGFNQWTIN
                     GIPYPDTAMMASPLFHLKEGKRYRIRMRNASDDIHPVHLHRHSFELTRIAGQATSGLM
                     KDVVMLGGYQEAEVDFIANNPGMTLFHCHQQLHMDFGFMCLFDYV"
     gene            6170..7528
                     /locus_tag="VFA02_06215"
     CDS             6170..7528
                     /locus_tag="VFA02_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09482.1"
                     /translation="MRTNSFRTQPSGDGRLTLAIVIQEAEAKYPAIRAAQEQQEAARN
                     AIGVAKTAYLPRADVLWQANRATTNKPNIAPLPQGIVPIPSTPARATTGHSDWNTLTG
                     VLLAWQPFDFGVRHAQVGIARFAYQSAQHSTDLSRLDVASAAAGAYLDVVTGHQMVIV
                     QQANVDRMEAFAKTVHVLVDNKLRPGADASQADAQLALARTQLIQAQTQEQVRLEALA
                     NLLQTSSQQIVIDGKDVLSTPSASTLNETPIEAHPQAEQLAALLNQQKEQVHLLNRSY
                     VPTFTLYGSASGLGAGLSSTNSSPVFEGGTSGLAPTEFNWMAAFQVTFPAFQIFTIHR
                     QTEQQKAQVLSTQSSYQQLLGDLSAQVKEAKAMLDGARRVASNTPVEVAAARDAERQQ
                     RARYKSGLATVVDVTAAEAALAQAEGDDAVARVNIWRGLAGVAEAQGDLTPFLQLLNK
                     QP"
     gene            7555..10737
                     /locus_tag="VFA02_06220"
     CDS             7555..10737
                     /locus_tag="VFA02_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU09483.1"
                     /translation="MWLIRAALRRPVTVIVFAAALALLSVIALLRTRIDIFPSLDLPT
                     IYVAQPYGGLSPQQMEGFIDYYYEYHFLYINGIQSVESRSIQGTGLIRLNFHPGTDMS
                     QALAQTISYVNRARAFMPPGTVSPFVIRFDAGTLPVGYMVFSSPSRTLSEIQDLALNR
                     VRPAFATLPGVSSPPPFGANQRTIILNVNPQKMHAYGMSPDDVVKALSSGNSIQPAGN
                     ADIGTTNTLVTTDSTVTNYDDLLNIPLHLGSGAAVYMRDVASISDTSDILAGYALLNG
                     KRTVYIPVTKRPDASTITVVNEVRNSLDRFQSLIPSDIKITYEFDQSQYVRAALGSVL
                     REALIGAALTGLMILLFLRDWRSALIVVITIPFALISSVVALSVTGQTINIMTLGGLA
                     LAVGVLVDEGTVLLENIHVHLEAGETKARAVLLASKEVATPRFLAMIAVFAVFLPSFF
                     MTGPARSLFLPLSLAVGFAMGASYVLSSTLVPVLTNWFLREIHHEDSAPNHGKQESEH
                     GFALFRKRFHGVLERIMDHPVLLLTGYAAFVLLVFLLVGPNLAQEIFPSSASQQFRLR
                     IDAPDGTRVAVTEDLVKQVLGVISHDAGDNNLDLTLSYIGTQGSSYPINAVFLWTSGS
                     HQAVVNVGLRPGSSIKLANLEEQLRKDLAQQFPTLRFSFDPGDLISQILNFGTPSLAE
                     VAVSGPQYADVQTYAEKVREHIAQLKSLRDVQIEEPLHYPTVDVRINRVVAGQLGATA
                     DQVGAAIGSATASSRFVSPNYWRDPRSGVSYQVQVQVPQTQMTSIEDIANIPVSSSTG
                     AHPLLSQVAQLRPTTVPGELDRINGLWTLEISANVGGNNLGAAARDLRRAIKNAGEPP
                     RGVSVTMRGQVNALSGIFGELSIGLAAAIVVILLLLTANFQSFKLALVVVSTTPAVLC
                     GVILMLFITGTTLNLQSFMGTIMAIGVAVANAILLVTFAEQNRRNGVESHAAARSAAA
                     ERLRPVLMTSLAMIAGMIPMALAIGQGSDEVAPLGRSVIGGLAVATLATLFLLPTIFG
                     IVQSRSPIGSRSLDPEDPESRFAQPMEPSR"
     gene            10848..11888
                     /locus_tag="VFA02_06225"
     CDS             10848..11888
                     /locus_tag="VFA02_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09484.1"
                     /translation="MKTVAVVSKKLDAPVSLPAQLLPYETVDVYPKVTGFIETIKVDR
                     GSRVKKGDVLVRLTAPELVAQRSQAESALHAAESQLASAQAKLAADQGTYLHLASAAK
                     TPGVVAENDLLVANQTAVADKGAVAAAESNANAARDALRSVTQMEAYLTIVAPFNGVV
                     TTRNLHPGALVGPASGPAGAMPIVQIVGTDRLRLVVPVPEALVGIMKEGQAVSFTVPA
                     YPGQSFHAPIARISHDIDVNTRTMPVELDVHNPDGRLSPGSFATVTWPVQRGYPTLFV
                     PTTAVTSDQQHSFVIRVRNGKTEWVTVQTGQVVNTEVEVFGRLQAGDAVVRTASDSIR
                     DGQEVSTEAAKQ"
     gene            11986..12282
                     /locus_tag="VFA02_06230"
     CDS             11986..12282
                     /locus_tag="VFA02_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU09485.1"
                     /translation="MHVLHHAADGPIFGLGIIEELGRHGYRLGPGTIYPLLHSMERRG
                     WLRGDAEIVEGRKRKIYEATTEGRRALNLARHRVRELYEEMFEQSLSSNKKARK"
     gene            complement(12380..12928)
                     /locus_tag="VFA02_06235"
     CDS             complement(12380..12928)
                     /locus_tag="VFA02_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09486.1"
                     /translation="MRCLLFAVFCALCTTAFHGQEIINVPRGSAIMVDGKVGPDEWSD
                     ARKTALANGVFLYSKLHEHFLLLAVVFPDGNYGFTDLLVNHDLDLHASAKLGERKQNS
                     NGTWPEWTWWNNNGWTANVSRVEEFAGPKLIPENVREYQIDLRRFPGGDLLVEVVSTV
                     LRETQVRAEWYSGPLLLKFTRQ"
     gene            complement(12972..13613)
                     /locus_tag="VFA02_06240"
     CDS             complement(12972..13613)
                     /locus_tag="VFA02_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09487.1"
                     /translation="MNRHENKAVRILIVDDHPVVQAGLASMLATHKDIEVIGSASSGE
                     EALTMAQQDTPDIILLDLRMPGMSGIDTLRALKEAQTTARTIILTSFETDENIYRAIQ
                     AGAQGYLLKDTSQDQMLEAIATVHAGKRYIPRPIAARLAERMLRSDLTARELEILEML
                     AKGLTNKQIGHALAISENTVRNHVNSIMEKLEVSDRTEAVAVAIQQGIIQVAS"
     gene            complement(13600..15738)
                     /locus_tag="VFA02_06245"
     CDS             complement(13600..15738)
                     /locus_tag="VFA02_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU09488.1"
                     /translation="MKRRPQIWKWLAISLSSLAVLAGAAFLFLLYLHSPKHGLPYHDS
                     FAQGKADEWEAFGGTWAVYDGGMRNDSDERGAKLMSGSPYWKNYLLEADVMLLGEDGD
                     AGLIIRASDEELGVDSYSGYYAGLRTRDNMLTLGRASHGWLEYQNVPMPESVQAFRWY
                     HLKLLAYECQIVASATFPLSGGHTTTAAMADKDCLSSGRIGLRSYSAGGIWRNVHARL
                     ATEDDLLAMLGNKTRQPFSSERAAGNGITIFGSGSLPSHVNQQVRPATNVQPISGLRL
                     VSGSQPAPITIRGVVSLTAPLYVQDSTGGIAVKAASSPPLKVGDEVQATGTVRSGAFH
                     SNLENATVQLLWARTPVQPVSVTASQAATGAFAAMFIEVEGHLLSKERDAQNTLVLNL
                     EDGQQSFRALVHGGRGDLLFNKLKLGSLLRLRGVCVVDSEYTNNLTPFVLLLRSTDDI
                     EVVTGPPWWNTRHVVAAGFALLLLLFIGQFIHSRIEQWRLRAILEEREMLAHEMHDTL
                     AQSFAGLGFQLQAIRNRMPEDMPAVHKQLDLACDLVRHSHEEARRDIMMLRSESFEQM
                     ELTRMLEQCARKMLDGGPVEIEVSQAGDIRPIPLRVNDTLFRIGQEALANAVRHATPT
                     RISIATAYAKDFVQMVIEDDGTGYDAGSEKKGFGVRGMKRRAAAISAALEIATSPNKG
                     TRVQVTAPLPARMAFRWKTHPKGSASHEQA"
     gene            complement(15735..16685)
                     /locus_tag="VFA02_06250"
     CDS             complement(15735..16685)
                     /locus_tag="VFA02_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU09489.1"
                     /translation="MVLSCARRISAPLLFVLAIGCRSGPPVIAVVPRTTGMSLWEPEH
                     TGAEAAAGAHGMKIYWNAPTREDDVQGQIALVEKIVDQRYAGLVLAPDQSLALMTPVR
                     RAVSKGIPTVVIGSALPLEPGGRLSYIVSDDEEAGHMAALRMGAILHGRGMVAIIGIN
                     PDIAGVLDRVRAFEATLAAQFPAIHIAEKRMGSFNVPYEQQITEEVLIANPNLNAIFG
                     VTADATRGAYSALSELHKAGAVKLVGCDQDLLAPLMTGEMDSVIAEDTYEMGRRAVEW
                     IALERQGKPVPPFQKIKPRLVTKENLNSPDIRRILGLVAQ"
     gene            16859..>18249
                     /locus_tag="VFA02_06255"
     CDS             16859..>18249
                     /locus_tag="VFA02_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09490.1"
                     /translation="MLNRREFVSMLAAASAALKTQASFAGAGLPNAASVTANSPGGGF
                     PLSDYTPFGYLDNPWHTWNMHQSGVLRSLPGIGFGLYYPAGPGGYFDYHRNGVYVAEL
                     ALGFRIGDRVFINPEDFRPEQLTSSHHSKNILQYRFEEGGIAIESSFFLVNEDALATY
                     VKIIEQSGRAQSIEVLALHAYQLGGSQWWGGDGIAGDFDQDAGALWMRSFAAGTVFAV
                     TADLHPVEHFFSAKEEDRRTWLSSNTKAGEKLAYSTQPLYSGIRYGLSLAPHAQKEFF
                     ILMARGANLPAALKHARASLQQCAAVLREKLSEDASFWNSAPRLTGDWPKHWQHGWVY
                     DFETLRTMVRRPIGLYKHPWDAMQIQAPRNVLAETSMDMWALSYANPTMAKEVFLGQF
                     LDAIEDNVPCMREDGVMNMVAADGSECGTAISWCFPFFCAESIYNRTRDLAWLRQLYP
                     RLSAFLRWTLRHRT"
BASE COUNT         4125 a         5119 c         4940 g         4065 t
ORIGIN      
        1 cctttgggaa ttttgatgcg cgcctcaatg cgtccgtact gaaagcttct gagcccttga
       61 gatttaagcc gcgcggaggt gtagtggcca tgctcatctc tgcgggccac gatgtgcaga
      121 tgacctttcc agacgaaaac gttgggccgc gaagagtcac atggcggcct ttgggaagca
      181 taggcgcagt agtcttccag ttcatgattg ccccagccac cggccccggt ctcgtatgtc
      241 cagttgtgcg ggtcgggagc agatggcgtt gtactgttgt tgaactcatc gctccagaca
      301 agcttctggc ccggcgctga aaaaaccggc gagcagaagg ccaggcacag agcaatcttc
      361 cagaaatgcc gcatcatgca catctttgct gaatccttgc ccggggacaa agtatcataa
      421 aagtatgctg gtaaatttcc ctcctcgcga ttctgtctca gaggagcaga tcgcaaccct
      481 tgtagatacg ttttataccc gcgtgcgtca ggacgaggta cttggccctg tctttgagca
      541 tgtaattggt gacgaatggg taccccatct ggaaaagatg cgggccttct ggtcttctct
      601 ggtgctggcc agtggccgct ataagggcaa tcccatgatg gcgcatctga tgatcgcgcc
      661 ccgcatcggc gcagaacact ttcagcggtg gctgaatctc tggcgccaga caacagcaga
      721 aattttcccg cagcagatag ccgcgatctt tgtacgtaag gctgaaagca tggcagaacg
      781 cctgctggag accatagacc agtaccacgc ttctttggcc tttagccaga actaacggtc
      841 ttttccacca gtacaggggc ctctgccagt ttgcagcata cagacgccgc aaggcaccat
      901 gccccgcctg ctacgcccac atccttcaga aaattcgatg tgaggatgtt gcccgggtgt
      961 gccgccagcg gagggcccca tacgatcagc ccgaagacga gaatggtagc accgagcatt
     1021 gttccggcaa agaacagatt ccggcccagc aggatccata caccggtaat caggaatatg
     1081 cccccagtca ggtagtccca gaagggctgc cacggcagtg gagaatggac tgatgggagc
     1141 atcggcggaa taccaggcgc atagacggta ctggcaaaat gcagaatgcc aaagccaatg
     1201 acaggcaggg caaagaggat tcggccaagc gtaagggccc ggtttcggcc agagacctgg
     1261 gtcccggcaa acagaaacgc cgctccgctg agcgcgaggt ctttgcaggc attgctgatc
     1321 cggccataca aaggaaaatc aaatatcttc tgatgtgcgt aagcatcccc gggcaaagat
     1381 aagagaaggc gggtgtgaat cagcacgacg aacgccagga ccatcgcacc aagcaacaca
     1441 cccgccagcc aggccacttt gcgcgctacg attgccagtc cggccgcaat cagcgcagtg
     1501 cctgtgaaat aaatccaaaa caggtgacag gggacccacg gcggcacgaa tgaagtaata
     1561 aaatgcgcat accgccagtg gacaatgcca taggcaatca tcgggagggc tagaagaaag
     1621 cgtcctgatt gcagaaacag acggattgcg cgatagcctg ccatgaagtt gcagacatgg
     1681 aaaactgtgg gcgatagaat gtccatctct attcgccaga agggtcagga acgtcggcag
     1741 gacgaaatat caagtattct gatgtgagac ggatgccgaa aatgaagcct gcttcatctc
     1801 tcatctgcta cattaagttt ctgcgctaat ctataaaaat taagcagatt gccttttaga
     1861 atgaaagctt ttgtaaccgg ttctacggga tttgtcggca gccatattgc caaggcactc
     1921 gccaaagaag gagcgcacct tcatcttctt gtgcgcagga acagcaatat ggcgaacctt
     1981 tccggcatgc atgctccgcc atcgttttcc acaacgaccg gagaccttct ggagccggaa
     2041 tctctgcgcc cgggcattcg tgggtgcgat tatgtctttc atgttgcggc agattaccga
     2101 ctgtgggtgc gcgatccaaa acaaatgtat gccgccaacg tggacggtac acgcgaactg
     2161 ctgcgtatct gccgcgaaga aggcgtaaag cgcgtgatct atacgtccag cgtggcgacg
     2221 atgggcttca ggaaagatgg caccatcgtg gacgaaaata cgcctgtctc tctgaacgac
     2281 atggtaggcc attacaagcg gtcaaaattt ctggccgagc aggaagccct gaaagccgcc
     2341 tatgatggac aggaagtgat ggtattgaac ccgacgaccc ccatcgggcc aggcgacatc
     2401 aagccaaccc ccacaggccg catcatagtg gatttcctga agcgcaagtt ccctgcatac
     2461 atggacacgg ggctgaacct ggtggatgta gatgaagtgg cgcgtgcaca tatagccgcc
     2521 atgacccgcg gtacaccggg cgaacgttac attcttggcg gcgagaacct gacgctgaag
     2581 cagattctgg acaagatgtc cgccattacc ggacttccct cccccagcgt gaaggtgccg
     2641 catacggtgg cacttatttt tgctttcttt gacgaaacca tcaatgggcg tcttcttggc
     2701 aaggagcctc gcgcaaccgt tgaggccgtg cgcatgggca ggaaaaaaat gtttgcatcg
     2761 tcggcaaaag ctgtgcgaga gcttggattc aaacaggtgc cggtttatca tgcgctgcgg
     2821 gcagccattg actggttccg cgccaacgga tatgcgccat gaagcgtatc ggaattgtgg
     2881 ccgcgctgca tggcgaactg aaacctttgg tgcgcggatg ggaaaaacgc gggcagctct
     2941 ggattggccg gacaggcgaa acgcactgca tggctactgc cggaggcatg ggagcacggg
     3001 ctgcagcacg cgcctgtgaa gtgctcctta aagaaggccc gctggatgcg cttatctcag
     3061 ttggatgggt tggagcgctt tcctgcggat tgaaaccgcc gcaggcccat gtcatctcag
     3121 aagtgataga tgcacgcaca ggcgagcggt ttgtgaccgc atcgggtgaa ggccagagac
     3181 tgctgacgac cgggcatgtg gtccgtccgg acgaaaagcg caagcttgcc caacagttcc
     3241 agactccgtt agtggatatg gaagccgcag cagtagcgcg catcgcccgc gaacagcagc
     3301 ttcccttcta ctgcttcaag gcaatctcag acggctacac ggaccgcctg ccggatttca
     3361 acccatttat cggccccgag ggccagatgc gcatggcgaa ttttgtggcg tatgcgatgc
     3421 tgcatccaca gtactggggc gcgttgatgc gattgggcag aaacagcagc atcgcagcca
     3481 ggaaccttgc agcactcatc atgcggcaac tttgaaacaa gaggatgaaa tcactttcag
     3541 aagaaaacgc caaggatgcg aggggcactc ggagatcggc cttccgggac tggatgtaga
     3601 acgtcgcgcc ggacagcgtt ggcagaaaat cccggccgtg gtctacacct gtgattctgt
     3661 gatgatgcct gccacaacga tgagcgagat ggagatgagc atttcgactg ccacgatggc
     3721 cgtgtaagga atgatgccat cgcgccagga aaccggaaac ggtcagtatc gaatatcgat
     3781 actttctctc tcgtcgctgc tccgctcccg tatatcccag aaacgccgca acccaatact
     3841 tgcccgtctt gtccgcaacg atacggaacg cagtccatag ccgaccatct cgccgaaccc
     3901 tgccacaaag cctacgaatg tcgcgcttgc gccaagagag ccgagaaatg gcctatgatg
     3961 cttcgagcgc cctgcgttcg agtcacttgc ttcggagacc gagtttctca tcgagagaaa
     4021 atcgtccgct cccgaaaaag agcagcgaga tgagtccaac gaacatgcag aaatctgtgc
     4081 gcccatcgtg cattgctgcc cagaatcctt gtttcagcat catcggaagc tttgtggtcg
     4141 cgattgcaac cgcaatgtcg atcaaaagcg gtaaagaggc ccaacgagtg aagaacccga
     4201 cgatgagaag ggttccgcaa acaatctcaa caactccgac aaagggtcct gagaattgcg
     4261 gcatcgggat tccgatcttc agaaatcggc cggcgcccag cgctgcagga aacaggaact
     4321 tctgaattcc ctcggacaag aagacccatc cgacaagaac tctgaccagc aagatgcccc
     4381 acgaaatgcg cggagagaga agtccttctt gaacgctcat gcggttcctc catttttcgg
     4441 gcggactaga cgtagtcaaa aaggcacatg aatccaaaat ccatgtgtaa ctgctggtga
     4501 cagtgaaaaa gggtcatgcc agggttattg gcaataaagt ccacctcagc ttcctgatat
     4561 ccgccaagca tcacgacatc cttcatgagc cccgacgttg cctgtccggc aattcgggtc
     4621 aactcaaagc tgtgacgatg caggtgcacg ggatggatgt catcgctggc attccgcatg
     4681 cggatgcggt agcgcttgcc ttctttgaga tgaaacagag gactcgccat cattgccgtg
     4741 tctgggtaag gtattccgtt gatcgtccat tgattgaacc cgtcgagcgc ggcattccgc
     4801 ttggcgaagg tcatctcgaa cacatcgtcg ggctgtgctg tctgttccgc tgaattcgcg
     4861 aagtgcagat aattccagtg aacttgtttc ggagctatcc attggggctt gccctgctgc
     4921 cctgcatact caacgacaat gcccatgccg tgatgacggt cgtcgtcgtc catgtcacca
     4981 aggatccaaa cgccgggatg gttcatttca acgattgcag agattctttc agcagttccc
     5041 aaccagagaa cgggtacctg ggccggtttg gggaccgggt ttccatccat tgccacgact
     5101 gagaatgcgt gcccaggaag agcaaggctt cggatctccg tcgcacttcc gttcaagaca
     5161 tgaaaaagga ccctctcgcc ctgctttact cggattggtt caccgtggcc cagcatccgg
     5221 ccgttgattg tgaacgtgcg gtaattcacc tcaaatccgt gaggcttgcc ttccttgacc
     5281 gacgttgcca tctctgactc gccgcggtcc ttgagttcct tgacttcctg gccagcaaga
     5341 aagtcgttcg ccatgtctcc gccatggcta aaccagggtt cgaattcctt gagcgtgaga
     5401 aacacttcgc ggtcgtagtg gcccgggttt tgtttcggct cgatatagac cggaccgact
     5461 tgccctgtgt actgacctcg attcagatcg cccccggcgc gaagatgcgt gtgatagaaa
     5521 cgcaatccag atggctgtgg gacaaaggaa atgcgtctca ttccatgcgc gggcacgaac
     5581 ggggttcctt cttctgctgc cccgtctacg ttgaccggaa cgttctgacc gtgccagtgg
     5641 agttgttcag gaatgtccgt gtcgttgtga atctcaacag tggtctgttc gccttcccgg
     5701 aatctcagga gcgggcctgg aaactgccca ttgaaggttg tcgtggaaac ataacggttc
     5761 ttcccaattt cgatcagtcc attctgaatg tgcagcgtat actccgcttt ttgacccgcc
     5821 tgttcaggag cttgtatgga tgcttgtgca tcaagctgca gggaaggaat caatgccgca
     5881 gccgcgccag ccaacttgag gaagttccgc cgcgtggtcg aggatgattg cattcttcgc
     5941 ctcctctcgt atgaggaaaa caatatcgca agtcggtatc gaatgtcgcg ataccttctc
     6001 tctcgtcgac gctctctaga ttgacagcgt tgcgccctgc gtgcaattat cgaactccga
     6061 tgatgaccta atcgaaaaat tcaaatatga ggccgcttaa agtgaggcag cgacatcttt
     6121 tcgcgtatgc gggacttgca atcgcggtgg cattgccgac catgctggcg tgcgcacaaa
     6181 cagcttccgc actcagccgt caggtgatgg tcgtttaaca ctggcaattg tcatccagga
     6241 ggctgaggcc aaatatccgg caattcgcgc tgcccaggaa cagcaggaag ccgcccgcaa
     6301 cgcaattggt gtggcgaaga cagcctatct acctcgtgcc gatgtgctgt ggcaggcgaa
     6361 tcgcgccaca accaacaaac caaacatcgc gcccctgccc cagggaattg ttccgattcc
     6421 ttctaccccc gcgcgtgcca ctaccggcca cagtgattgg aatacgctga ccggggtatt
     6481 gctcgcatgg caaccgtttg attttggagt gcgtcacgcc caggttggca tcgccaggtt
     6541 tgcttatcag tctgcgcaac actccacaga tctgagccgc ctggacgtag catccgctgc
     6601 tgccggcgct tatcttgacg tggtgacggg acaccagatg gtgattgtgc agcaggcgaa
     6661 cgttgaccgc atggaggcgt tcgccaaaac cgtccacgtc ctggtggata acaaactccg
     6721 tcctggcgcc gacgcttcgc aagccgatgc acaacttgct ttggcacgaa cccaactcat
     6781 tcaggcccag acacaagaac aagtgcggct ggaggccctt gcaaatctgc tgcaaacctc
     6841 ttcgcagcag atcgtcattg acgggaagga cgttctcagc accccctctg catccacctt
     6901 gaacgaaacg ccgatcgagg cccatccaca agcggaacaa ctggctgcgc ttcttaatca
     6961 acagaaggaa caggtccatt tgctcaatcg gtcgtatgtg ccgaccttta ctctgtacgg
     7021 gtctgcttcc ggtttaggcg cggggctttc gagcacaaac tcatctccgg ttttcgaggg
     7081 tggaacttcc gggctggctc ccactgaatt caattggatg gcagccttcc aggtgacatt
     7141 tcctgcgttt cagatcttta ccatccaccg acagacagaa caacagaagg cacaggtcct
     7201 gtccacgcag tcttcctatc agcagttgct tggggatttg tctgctcagg taaaggaggc
     7261 taaagcgatg cttgatggcg cgaggcgcgt ggcctccaat acacctgtcg aagtggcggc
     7321 cgcgcgcgat gccgaaagac aacaacgagc gaggtacaaa tctggcctgg caactgttgt
     7381 tgatgttaca gcggcagagg ccgccctcgc ccaagcggag ggggatgacg ctgtcgcacg
     7441 cgtgaacatc tggcgaggcc ttgccggagt cgcggaggcg caaggagact tgacgccatt
     7501 tcttcagttg ctgaacaagc agccgtaaac ggacttcgcc aggagtaaga atctatgtgg
     7561 cttattcgtg ctgcactacg cagacccgtt accgtgatcg tcttcgccgc cgcgcttgca
     7621 ctgttgtcag tgatcgcgct cttgcgtaca cgcatcgaca tcttccccag ccttgatctg
     7681 cccacgattt atgtagcgca gccttacgga ggcttgagcc cgcaacagat ggaagggttc
     7741 atcgattact actacgagta ccacttcctt tacatcaatg gcattcaaag cgtcgaatcg
     7801 cgatcaatcc aagggacagg actgattcgt ctcaactttc atcccggaac cgacatgagc
     7861 caggcgctgg cccagaccat ttcatacgtg aatcgcgcac gggccttcat gcctccggga
     7921 accgtttctc cgttcgttat tcggtttgac gcgggaacac tgccggtcgg atacatggtg
     7981 ttctcaagcc cgtcgcgcac gctgtctgag atccaggatc tcgcgttgaa tcgggtccgg
     8041 cctgcgttcg caactcttcc cggcgtctcg tcgccgcctc catttggcgc gaaccaacgc
     8101 accattatcc tcaatgtcaa tccgcagaag atgcatgctt acggaatgtc tccggatgat
     8161 gttgtgaagg ccctcagcag cgggaatagc atccagccgg caggcaatgc agatatcggt
     8221 acaacgaaca cgctggtcac cacggattcg acggtgacga attacgatga tctgctgaat
     8281 attcctctgc atcttggctc cggcgcagca gtttacatgc gagacgtcgc cagcatctcc
     8341 gatacttctg acattcttgc cggttacgcg ctgttgaacg ggaagcgtac cgtgtatatt
     8401 ccggtgacca agcggcccga cgcatccacg atcaccgtgg tcaatgaagt ccgaaacagc
     8461 ctcgaccggt tccaatcgct gattccatct gacattaaaa ttacctacga atttgaccaa
     8521 tcccaatacg tgcgcgccgc actgggctcg gtgctgcgtg aagcactgat cggcgccgca
     8581 ttgaccggac tgatgatcct gcttttcctt cgcgactggc gatcggcgct gattgtggtc
     8641 attaccattc cgtttgcact gatatcgtct gtcgtcgcat tgagtgtcac cggccaaacc
     8701 atcaacatca tgacgctcgg aggcctggcc ctggctgtag gcgttctggt ggacgaaggg
     8761 acggtgcttc tcgaaaacat ccacgtacat cttgaagccg gggagacgaa agcgcgagca
     8821 gtcctactgg caagcaaaga ggtcgccacg cctcggtttc tcgcaatgat tgctgtattc
     8881 gctgtgttcc tgccttcatt cttcatgacc ggcccggcgc gctcgctgtt cctgccgctg
     8941 tctctggcag taggcttcgc gatgggtgct tcctatgtgc tctcaagtac tctcgtgccc
     9001 gttttaacga actggttctt gcgcgagatc catcatgagg acagtgcgcc caatcacggg
     9061 aaacaagaga gtgagcatgg gttcgcactg tttcgcaagc gctttcacgg tgtgcttgag
     9121 cgcatcatgg accatccggt gctcctgctg actggttacg cagctttcgt gttactggtc
     9181 tttctcctgg tcggaccgaa tctggcgcag gagatctttc cttcctccgc gtcccagcag
     9241 ttccggctgc ggatcgatgc cccggacggc acgcgcgtgg ctgtcacgga ggacctggtc
     9301 aaacaggttc tcggcgtaat cagtcacgac gctggagaca acaatctcga tctgaccctc
     9361 agctacattg gaacccaagg atcttcgtat ccgattaacg ccgtctttct ctggaccagc
     9421 ggatcgcatc aggccgtagt taacgtcggt ctaaggcccg gtagttccat caagctggca
     9481 aatctcgaag agcaactgcg caaagatctc gcgcaacaat tcccaaccct ccgtttctcc
     9541 tttgatcctg gcgacctgat cagccagatc ctgaactttg gtacaccgtc attggccgaa
     9601 gtggcggtca gcgggcctca atatgccgac gtacagacct acgccgaaaa ggtgcgcgag
     9661 catatagctc agttgaagtc gctgcgcgat gttcaaatcg aggagcccct gcattacccc
     9721 accgttgacg tacgaatcaa tcgcgtggtc gcgggacagt tgggagcgac tgcggatcag
     9781 gtaggcgctg ccataggatc ggcaaccgca tcaagccgat ttgtttctcc taattactgg
     9841 cgcgacccgc gctcgggcgt cagttaccag gtgcaggttc aggtaccaca gacacagatg
     9901 acgtcgatcg aggacatcgc gaacattccg gtgtcgagtt ccaccggcgc tcatccattg
     9961 ctcagccagg ttgcgcagtt gcgccctacg acagttcccg gtgaactcga ccgcatcaac
    10021 gggctttgga cgttggaaat ctctgccaat gttggcggca acaatctggg cgcagcagcg
    10081 cgtgaccttc ggcgagcgat caagaatgcg ggcgaaccgc cgcgcggtgt ttctgtgacc
    10141 atgcgtggac aggtcaacgc gctgagcggg atcttcggcg aattgtctat cggcctcgcg
    10201 gcggctatcg tcgtgatctt gctcttgctg acagcgaatt tccaatcctt caaactcgct
    10261 ctggtcgttg tctcaacgac cccggcagtg ctgtgtgggg tcattttgat gctgttcatt
    10321 accggtacaa ctctgaatct ccagagtttc atgggaacga tcatggccat cggtgtcgca
    10381 gtagccaatg cgattctgct cgtcaccttc gcggaacaga atcgcagaaa cggcgtcgaa
    10441 tcgcatgcgg ccgcacgttc cgctgccgct gagcgtctgc gtcctgtgct gatgaccagc
    10501 ctcgcgatga ttgccggtat gattcccatg gcgcttgcta tcgggcaagg gagcgatgaa
    10561 gtagctcctc ttggccgctc tgtcatcggc ggtctggcag tggctaccct ggcgactctg
    10621 ttcttattgc cgaccatatt cggtatcgtc caaagccgct ctcctatcgg ttcccgttcc
    10681 ctcgatccag aagatcccga aagccgcttt gcccaaccta tggagccctc gagatgaaga
    10741 gactagcccg catatcatct tgcgctttaa cgcttttagt tcccgtcctg acaggctgcg
    10801 acggaggaaa gcctgctgcg tcaaatgtgg cgcaagcggc tgtctcagtg aaaacagtcg
    10861 ctgttgtctc gaagaaactg gatgctcctg tgagcctgcc tgctcaattg ctgccctatg
    10921 agaccgtgga tgtgtatccg aaggtcactg ggttcattga aaccatcaag gttgaccgcg
    10981 gctcccgcgt gaagaaagga gatgttctgg tacgccttac cgcgccggaa ttggtcgcgc
    11041 agcggtcgca ggccgaatcc gcactacacg cggccgagtc tcaacttgcc tcggcccagg
    11101 ccaagctggc agccgatcaa ggaacgtacc ttcacctggc ttcggcggcg aaaacgccgg
    11161 gagtggtcgc agagaacgac cttctggtgg caaatcaaac agccgttgca gacaagggtg
    11221 cggtcgccgc agcggaaagc aacgccaacg ccgctcgtga cgcgctgcgc agcgtgacgc
    11281 agatggaggc ttacctcacc atcgtggcgc cattcaatgg agtggtcacg acacggaacc
    11341 tgcatcccgg cgctctcgtt ggacctgcct ctggtccagc cggcgcaatg cccattgttc
    11401 agattgttgg cacagatcgc ttgcggctgg tcgtgcctgt tcctgaagcc ctggtaggaa
    11461 tcatgaaaga aggtcaggca gtcagcttta cagtaccagc ctacccggga cagagcttcc
    11521 acgcaccgat tgccagaatc tcgcacgaca tcgatgtgaa cacacgtaca atgccagttg
    11581 agcttgacgt tcacaatccg gatggacggc tctcgcctgg aagctttgct actgtaacat
    11641 ggcccgttca acgaggttat ccgaccctgt tcgttccgac gacggcggtc accagcgacc
    11701 agcaacacag ttttgtgatc cgtgtacgca acggaaagac tgaatgggtc acagttcaaa
    11761 ctgggcaggt agtaaacacc gaggtggaag tgttcggccg cctccaagca ggagacgctg
    11821 tcgtgcgcac cgcttccgat tcgattcgtg acgggcaaga agtgagcaca gaggcagcaa
    11881 agcaataacc acgatcttaa ttcggctttg cccgagagtg atgacacaat gacttcaagg
    11941 gacaccaaag gacgaaatca agagctctac atcgggctcg tacgattgca cgttttgcat
    12001 cacgcagcag atggcccaat cttcggtctc ggcatcatcg aggaacttgg ccggcatgga
    12061 tatcggctag gccccggcac catctatcct ctgcttcaca gcatggaacg cagaggatgg
    12121 ctacggggag atgcagagat cgtcgaaggt cgcaagcgca agatttatga agcgaccaca
    12181 gaaggtcgca gagcgctcaa cctcgcgcga caccgggtta gagaactcta tgaagagatg
    12241 ttcgaacaga gtctcagctc gaacaaaaag gcccggaaat gattctgagc agtcgagatc
    12301 tccctcagtc caaggtccgt tctgctgtcg ggaccatcca tatatagcgc tcgccataga
    12361 cgctatcggg tataaacaat cactgccgtg taaatttcaa caggagcggg ccggaatacc
    12421 attctgctcg aacctgggtt tcacgcaaaa ccgtggagac cacttcaacg agtaggtctc
    12481 ctccagggaa acgcctgagg tctatctggt attcacggac attctcgggt ataagcttcg
    12541 gtcctgcaaa ttcttctacc ctggaaacgt tggctgtcca gccgttattg ttccaccatg
    12601 tccactcagg ccatgttcca ttagagtttt gtttgcgttc gccaagtttg gcagaagcat
    12661 gcaaatccag gtcatggttc accaaaagat cagtaaagcc ataattccca tctggaaaga
    12721 caactgctaa taacaaaaag tgctcatgca gtttactgta gagaaaaacg ccgttcgcca
    12781 gagcagtttt tctggcgtca gaccactcgt ctggcccaac cttcccatca accatgatgg
    12841 ctgacccgcg cggaacatta attatttctt gcccgtgaaa agcagtcgta caaagcgcac
    12901 agaaaacagc aaacaaaagg catctcataa agtcttacct catcgccaca tttatgtttg
    12961 caagttaatc gtcagctcgc gacctgaata attccctgct gaattgccac ggcgacggct
    13021 tctgtgcggt cggaaacctc cagcttttcc ataatactgt tgacgtgatt gcggacggtg
    13081 ttttcgctga tggcgagagc atgaccaatc tgcttgttcg taagcccttt tgcgagcatt
    13141 tcaagaattt ccaattcgcg ggcggtcagg tctgagcgga gcatccgctc tgcaagccgt
    13201 gccgcaattg ggcgaggaat atatcttttt cctgcatgga ctgtagcaat cgcttcaagc
    13261 atctggtcct gcgaggtgtc tttcagcaga tatccctgcg ccccggcctg gatggcgcga
    13321 tagatgtttt cgtcggtttc aaagctggta agaatgatgg tccgtgccgt ggtctgggcc
    13381 tctttcaaag cgcgcagcgt atctatgccg ctcattcccg gcatacggag atcgagcaat
    13441 atgatgtcgg gcgtgtcctg ctgcgccatt gtcagcgcct cttcgccact cgatgctgag
    13501 ccaatcactt cgatgtcctt atgcgtggcc agcatacttg ccagtccggc ctggactacc
    13561 ggatggtcat caacaatcag aatacggact gccttgtttt catgcctgtt catgagatgc
    13621 gcttcctttg ggatgtgtct tccatctgaa agccatgcgg gcaggcagcg gtgccgttac
    13681 ctgtacgcgt gttcctttgt taggtgatgt ggcaatttca agtgcagcag agatggctgc
    13741 tgcacgtctc ttcattccgc gcacgccaaa gcctttcttt tcactgcctg cgtcatatcc
    13801 agtaccgtca tcttcaatga ccatctggac aaaatctttc gcgtaggctg tagcaataga
    13861 gatgcgcgta ggtgtggcat ggcgcaccgc attggcgagc gcttcctgcc cgatgcggaa
    13921 cagagtgtca ttcacccgca aaggaatggg gcgaatatct ccggcttgcg acacctcgat
    13981 ttcaacaggc cctccatcca gcattttcct tgcgcattgt tcaagcatac gagtcagctc
    14041 catctgttcg aaggactcgc tgcgcagcat catgatgtcg cgccgcgctt cttcatgact
    14101 gtggcgcact aaatcacagg caaggtcaag ttgtttgtga acggcaggca tgtcctcggg
    14161 catacgattg cgaatggcct gaagctgaaa gccgagtccg gcgaagctct gcgccaaagt
    14221 gtcgtgcatt tcatgcgcaa gcatttcgcg ctcttccagg atggcgcgca gccgccactg
    14281 ttcgatccgg ctatggatga actggccaat aaaaagaagc agcagaagag caaatccagc
    14341 cgccaccaca tgccgcgtat tccaccatgg aggtccggtg accacttcaa tgtcatcggt
    14401 ggagcggagc agcaggacaa atggagtgag gttgttcgta tattcggaat ctacaacgca
    14461 tactccacgc aggcgcagaa ggctgcccaa tttcaatttg ttgaagagca ggtctccgcg
    14521 cccaccgtga acaagcgcac ggaaagactg ctggccatct tcaagattca gaaccagcgt
    14581 attttgcgcg tcgcgctctt tgctgagcag atgcccttcg acctcaatga acatcgcggc
    14641 aaatgctccg gttgcagcct gcgatgcagt cacggaaaca ggctgtaccg gcgtccttgc
    14701 ccacaataac tggactgttg cattttccag gttggaatgg aaagcccccg accttacggt
    14761 tcctgtagcc tgtacttcat caccaacttt caatggagga ctcgaggctg ctttaacggc
    14821 aatgcctccg gtagagtctt gcacatacaa cggcgctgtc agcgagacaa caccccgaat
    14881 cgtaatgggc gcgggctgcg aacctgaaac cagacgaagg ccgctgatgg gctgaacatt
    14941 cgttgctgga cggacctgct ggtttacatg cgaaggcaga gaaccagaac caaaaattgt
    15001 aattccattt cccgctgctc gttcgctcga aaatggctgt ctggttttgt tgcccagcat
    15061 ggcgagaaga tcatcttccg tcgccagccg cgcatgaaca ttgcgccaga ttcctccggc
    15121 ggaataagac cgcagtccta tgcgtcctga agagaggcag tccttatcag ccattgcagc
    15181 ggtcgtggta tggccgccgc tcaatggaaa tgtagccgaa gcaacaatct ggcactcata
    15241 agcgagtagc ttgaggtgat accaacggaa tgcctgcacg ctttccggca tgggcacatt
    15301 ctggtattca agccagccat ggcttgcgcg tccgagtgtc agcatgttgt cccgcgtgcg
    15361 cagtccagca taatagccgc tataggaatc cactcccagt tcttcgtcgc ttgcacggat
    15421 gatgagtccg gcatcgccat cttctcccag cagcatcaca tctgcttcca gaaggtaatt
    15481 tttccagtaa ggagagccac tcatcagctt ggcgccgcgc tcgtcggaat cgttgcgcat
    15541 tccgccgtca taaacggccc aggtgccgcc gaatgcttcc cactcgtcgg ctttcccctg
    15601 cgcaaaggag tcatgatacg ggagtccatg tttcgggctg tgcaaataaa ggagaaaaag
    15661 aaaggccgcc ccagcaagca cggcaaggct ggacaaagag attgccagcc atttccatat
    15721 ttgcggacga cgcttcattg cgcaacaagt cctaatatcc gccggatgtc aggagaattc
    15781 aaattctcct ttgttaccag gcgaggcttg atcttctgaa atggcggcac tggtttaccc
    15841 tgacgctcaa gagcaatcca ttccacagcg cggcggccca tctcataggt gtcctcggca
    15901 atgacggaat ccatttctcc ggtcatcagc ggcgcaagca ggtcttgatc acagcccacg
    15961 agtttcacgg cccctgcttt gtgcaattca cttaatgccg agtatgcacc gcgagtggca
    16021 tccgcagtca cgccaaagat ggcattcagg tttgggttgg ctatcaatac ttcttccgta
    16081 atctgctgct cataagggac attgaatgat cccattcttt tttctgcgat gtgaatggct
    16141 gggaattgcg cggcgagtgt cgcttcaaac gcacggacac ggtccagcac cccagcgatg
    16201 tccggattga tgccaatgat ggctaccatg cctctaccgt gcaggatggc gcccatgcgg
    16261 agcgctgcca tgtgtccggc ctcttcatca tcgctgacga tatatgacaa cctgccgcct
    16321 ggttccaatg gaagggcgga gccaatcact actgtgggaa tgcctttgga aacagcgcgc
    16381 cgcaccggtg tcatcaaggc aagcgattgg tccggcgcaa ggaccagtcc tgcatatctc
    16441 tgatcaacaa ttttttccac cagcgcgatc tggccctgca cgtcatcttc gcgtgtggga
    16501 gcgttccagt atatcttcat gccatgcgcg ccggccgcag cttctgcgcc tgtgtgttcc
    16561 ggttcccaca aagacatgcc tgttgttcgg ggaaccactg caatcactgg aggaccagac
    16621 cggcagccaa tcgcgagaac aaaaagtaga ggggcagaaa ttctgcgcgc acaggaaagt
    16681 accatcacat tcagaatgca ctccgctgcg ggtatatggc cagtccattc atactatatg
    16741 gctatggtct gactggccca ggcactgttg acctgcctgc ccatatcgtc actttatcta
    16801 acattttgca aagaacagtt cccttccata tacgaatagc cataggagtt ctctctgaat
    16861 gctgaaccgc cgcgaatttg tctccatgct tgctgctgca tctgctgctc tgaaaacgca
    16921 ggcttccttt gcaggtgccg gcttaccgaa cgcagccagc gtcaccgcaa acagtccggg
    16981 cggtgggttt ccgctcagtg attacacacc ttttggctat ctggacaatc cgtggcacac
    17041 atggaacatg caccagagcg gtgttctgcg ctcgcttccc ggaattgggt tcggcctgta
    17101 ttaccccgca gggccaggag gatacttcga ctatcaccgg aatggagtct atgttgcaga
    17161 actggcactc ggcttcagaa ttggcgatcg tgtttttata aacccggagg attttcgtcc
    17221 tgaacaattg acatcttcgc accacagcaa aaatattctt caataccgtt ttgaagaagg
    17281 tggcatcgcc atagaaagca gcttctttct tgtaaatgag gatgctcttg ccacgtatgt
    17341 gaagatcatc gagcaatcgg gccgtgccca gtctatcgaa gtccttgcgc tgcacgctta
    17401 ccagcttgga ggaagccaat ggtggggagg cgatggcatc gccggagatt ttgatcaaga
    17461 tgcaggcgca ctctggatgc gatcatttgc cgccgggacg gtctttgcgg tgactgcgga
    17521 cctccaccct gtagaacact tcttttctgc aaaagaagag gaccgccgga catggctgag
    17581 ttctaataca aaagctggag aaaagctcgc ctattctact cagccactct acagtggcat
    17641 ccgttatggc ctttcccttg cgcctcatgc acagaaagag ttttttattc tcatggcgcg
    17701 cggggccaat cttcctgcag ccctgaaaca tgcgcgggct tcattgcagc aatgtgccgc
    17761 ggtcctgcgg gaaaagctca gtgaagacgc atcgttttgg aacagcgccc cacggctgac
    17821 aggggactgg ccgaagcatt ggcagcatgg atgggtctat gactttgaaa cgttgcgaac
    17881 aatggttcgg agacccattg gtctttacaa gcacccgtgg gacgcaatgc agatccaggc
    17941 gccacgcaat gtgctcgccg agacttcgat ggatatgtgg gccttgagct atgccaatcc
    18001 aactatggca aaggaggtct ttctaggaca gttccttgat gcgatcgagg acaacgtccc
    18061 ttgtatgcgt gaagatggcg tgatgaatat ggtggccgca gacggctccg aatgcggcac
    18121 ggcgatttcc tggtgctttc cgttcttctg cgctgaatcc atctataacc gcacgcgcga
    18181 cctcgcctgg ttgcgccagc tttatccacg cctgagcgct tttctgcgct ggacactcag
    18241 gcaccgcac
//
LOCUS       DASRBV010000162         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_618412, whole genome shotgun sequence.
ACCESSION   DASRBV010000162 DASRBV010000000
VERSION     DASRBV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618412"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="VFA02_06260"
     CDS             complement(<1..2367)
                     /locus_tag="VFA02_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09491.1"
                     /translation="MVRNAGGGPVSGATVQLTARSHKRTVTTAADGSFAIPDPKQDEY
                     HLSVEMDAKSFDSGTPLVPQADATVVITLAKDGTLSVSEQKDQQEATGGEQLSSKTVS
                     ELPLNKRDFSQLLLLAAGTMTDVNGATNFTQQFAINGQRGVEATFAMDGADTSDPEMG
                     GGTFTNFNVDAVEQIQSSSGWMPAEIGRGAAGFTNIITRSGKSGFHGSFFEFLRNSAL
                     DARNYFDHPPVGRIPPFRRNEFGFTNGGPVVLPHLYNGRGKTFYFGEYQGFRQVLGTT
                     QVLAVPTAEERSGINASAYPGDVLYVPVDAQIARVLTRYPLPNYPAGPYGEHTYATSS
                     KVYTNADQFSIRIDQKMDDKSEIFGRFTYDNINGPTTNPDQTAIDPSFAVEYIDRQRN
                     GVLTYTRTVSPRYSFESSISFTRTTPSFPTPNHTDPAVKFNDGLFEAFNSAAGSVMTA
                     FGNLFQARQNFSYVAGKHAWKWGGEVRLNRDTTYFGTSVNGEYDFGGGQVYSPEYIHS
                     QSGTHDVRAGDPLPDTLSALLTGSPFSYNVAVAPPYFSSGAHIGPAAINRNSFSAYVQ
                     DTWKISDRFTLDYGLRYEIYTPITERAKRTSGFMNVNGVQRFVVNPQPGYQTNYDGLG
                     PRVQLDWMMAKQAHLRVGGGITTIPPNIWQDNFLTGSTPFVVYPHLSVTRGAQFHYGF
                     QITPNQLPRAYSLSGQDIFASGDTKKVPANTIFDVDRYEHDMAALSPSHQIVPLNLSG
                     VERSFGNGMLETWTLGLERQFGNLTADLGYVGTAAAKLPRIIYPNGFPG"
     gene            complement(2736..>3873)
                     /locus_tag="VFA02_06265"
     CDS             complement(2736..>3873)
                     /locus_tag="VFA02_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU09492.1"
                     /translation="EQIGITPSASLARFTTTYGNVIYLYDHRWMPQASLPPGVDFSSL
                     PHVTGLFNIIAFVAIAVVTVVLVIGIKESANLNSAIVFVKVGVVGIFLVLGIGYLLQH
                     PDLARMNWHPFIPPKQGPGQYGISGIAAGAASIFFAYIGFDAVSTAAQEAKNPQRDMP
                     IGILGSLVICTILYIAVSLTLTGLVSYKALNVAEPVAIGIDATGVRWGGILVKIGAVF
                     GLGTVMLVMLLGQSRVFYSMSRDGLLPKWAGAIHPKFRTPWISNIVVGTIVAFLPALL
                     PIDKLSAMVNMGTLLAFAIVSAGVWILRVRQPELERPFRTPLVPLVPILGIISALYLI
                     ASLPRLTWIVVSIWLVVGLMIYFFYSTKHSKVQKKEAMITAGKS"
BASE COUNT          936 a         1130 c          992 g          815 t
ORIGIN      
        1 cccgggaaaa ccattgggat aaattatgcg cggcagtttt gccgcggccg tgcccacata
       61 gccaagatcg gcggtaagat ttccgaactg ccgttcgagc cccagggtcc aggtctcaag
      121 catgccgttg ccaaacgagc gttcaacgcc ggaaaggttc agcggaacaa tctggtgcga
      181 aggggaaagc gcagccatat cgtgctcata gcggtctacg tcaaaaatcg tgttcgccgg
      241 gaccttcttc gtgtcgccac tcgcaaaaat gtcctgcccg gagagcgagt atgcacgagg
      301 caactggttc ggggtgatct gaaaaccata atgaaactgc gcgcctctgg tcacgctcag
      361 gtgcggatag acaacaaacg gcgtagatcc agtaaggaaa ttgtcctgcc agatgttcgg
      421 cggaatcgta gtaatgcctc cgcccacacg cagatgcgcc tgtttcgcca tcatccagtc
      481 aagctggacg cgcggtccca gaccgtcata gttcgtctga tatccaggct gcggattcac
      541 tacaaaccgc tgcaccccat tcacattcat gaaaccagat gtccgtttcg cccgctcagt
      601 aattggcgta tagatctcat accgcaggcc gtaatccaac gtaaaccgat cagagatttt
      661 ccatgtgtcc tgcacgtaag ctgaaaaact attgcggtta atggccgccg gaccgatatg
      721 cgcgccactc gaaaaatatg gcggtgctac tgccacattg taggaaaatg gactgcccgt
      781 cagcagagca gaaagggtgt ccggcagcgg atcaccggca cggacatcgt gtgtcccgct
      841 ttgcgaatga atgtactcgg gcgagtacac ctgacctccg ccaaagtcat actcaccatt
      901 gacgctggtt ccgaagtatg tggtgtcgcg gttcagacgg acctcgccgc cccatttcca
      961 ggcgtgtttt ccggcgacat aagagaaatt ctgcctcgcc tgaaaaagat taccgaacgc
     1021 cgtcatgacc gagccggcag ccgaattgaa tgcctcaaat agtccatcat taaatttcac
     1081 cgcagggtcc gtatggttcg gcgtcggaaa ggatggagtg gtccgcgtaa agctgatgga
     1141 agactcaaat gaatatctcg gcgagactgt gcgcgtatac gtcagcaccc cattgcgctg
     1201 cctgtctatg tactcgacgg caaaactggg atcaatcgct gtctggtccg ggtttgtcgt
     1261 cgggccgttg atattgtcgt aggtaaaccg cccgaatatc tccgacttgt catccatctt
     1321 ctggtctata cgaatcgaaa actggtcggc attcgtgtac acctttgacg aagttgcata
     1381 cgtgtgctct ccatatggcc ccgccggata atttggtaat gggtaccgcg tcagcacccg
     1441 cgcaatctgc gcgtccaccg gaacatacag cacatcgcca ggatacgcac tcgcattgat
     1501 gccggaacgc tcctctgccg tcggaacagc cagcacctgc gtcgtcccca gcacctgccg
     1561 gaagccctga tactctccga aatagaaggt ctttccccgc ccgttatata aatgtggcaa
     1621 aaccacaggc ccgccatttg taaatccaaa ttcgttgcgc cggaaaggag gtatacgccc
     1681 caccggcgga tggtcaaagt aattgcgagc atccagcgcc gagttacgca aaaactcaaa
     1741 gaacgatcca tgaaaaccgc tcttgcccga acgggtaatg atattcgtaa atcccgcagc
     1801 gccgcgcccg atttccgcag gcatccatcc ggaacttgat tggatttgtt ccaccgcatc
     1861 cacgttgaag ttcgtgaatg tgccgccgcc catctccgga tcactcgtgt ccgcgccatc
     1921 catggcaaac gttgcctcta cgccacgttg gccattgata gcaaactgct gcgtgaagtt
     1981 cgtcgcgccg ttcacgtcgg tcatcgtccc ggcagcaagc agcaataatt ggctgaagtc
     2041 gcgcttattc agcggaagct cgctgactgt tttacttgaa agctgctcgc cccctgtggc
     2101 ctcctgctgg tctttctgct ctgaaacaga aagcgtgcca tctttcgcca atgtgatgac
     2161 cactgtcgcg tcggcctgcg gcacaagtgg ggttccgctg tcgaatgatt tcgcatccat
     2221 ctcaaccgag agatgatatt catcctgctt cgggtccggg atagcaaagc ttccgtccgc
     2281 tgcggtcgtg actgtgcgct tgtggctcct ggctgtcaat tggactgtcg caccactcac
     2341 aggaccgcct ccggcattgc gaaccacacc tctccacgcc gaatggcctt cctccttctg
     2401 cgcccggatg gaatgaaaag aaacgaacgc taccgccaga aatgcaacca gccagataca
     2461 gtttgcgcgc ctcacctcgg aaaaatgccc cgccttttct tcttttttga aacagcatgt
     2521 gcggattcat tatatgtgca cacgtccggg acaagatggc agagatggtg aacttctcta
     2581 tctggcaggc aaaccgtttg ttccccggta tgaaattaca agaggcagcc cccactcagg
     2641 cctttcagct tgaatgggga aacctatttg attacagcgg ccaggacgcc aggcggcctc
     2701 catgtctcga agagacgtgg gatatctccg gaatgtcagg atttgcccgc cgtaatcatg
     2761 gcttccttct tctgcacctt gctgtgcttt gttgagtaga aaaagtaaat catcagacca
     2821 actaccagcc agatactcac aacaatccac gtcagtcttg gcaggctcgc aatcaagtag
     2881 agcgcactga taattcccag gatcgggacc agcggaacta gcggcgtgcg gaacggacgt
     2941 tctaactcgg gctggcgcac acgcaggatc cagacgccgg ccgaaacaat ggcaaaggcc
     3001 agcagcgtgc ccatattcac catggcgctc agcttatcaa tgggcagaag tgcaggcaga
     3061 aatgcaacaa tcgtgcccac aacaatgttg gaaatccatg gagtgcgaaa ctttggatgg
     3121 atggcgccgg cccacttcgg cagcagccca tcgcgcgaca tggaataaaa tacgcgcgac
     3181 tgacccagca acatcactag catcaccgtg cccagcccaa acacagcgcc aatcttcacc
     3241 agaatgccgc cccagcgtac tccggttgcg tcaatgccaa tcgccaccgg ctcagccacg
     3301 ttcagggctt tgtagctcac cagccctgtc agcgtaagcg aaacagcaat gtacaggatg
     3361 gtgcagataa ccagcgaccc gagaatgccg atgggcatat cgcgctgtgg gtttttcgcc
     3421 tcctgcgcgg ccgttgagac agcatcgaat ccgatatacg caaaaaagat ggatgcagcg
     3481 cccgctgcaa taccgctgat tccatactgt cccggcccct gcttcggtgg aataaaagga
     3541 tgccagttca tccgggccag atccggatgc tgcaacagat aaccgatccc cagcaccagg
     3601 aaaatgccga ctactccgac tttgacgaag acaatggccg aattcaggtt ggccgattcc
     3661 ttgatgccaa ttaccaggac aactgtcacg acagcaatcg ccacgaaagc aatgatgttg
     3721 aacagtccgg ttacatgcgg cagcgacgaa aaatccactc ccggaggcaa agaagcctgt
     3781 ggcatccagc gatgatcata cagataaatc acattgccgt atgtggtcgt aaaccgcgcc
     3841 agcgaggccg acggtgtaat gccgatctgc tca
//
LOCUS       DASRBV010000163         7650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_313914, whole genome shotgun sequence.
ACCESSION   DASRBV010000163 DASRBV010000000
VERSION     DASRBV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313914"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFA02_06270"
     CDS             <1..465
                     /locus_tag="VFA02_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZU09493.1"
                     /translation="DGLFLKCAREVGKGFPEITYGEHIIDNTCMQLVLNPYQYDVLLL
                     ENLYGDIVSDLCAAFVGGLGLVPGANLGTEAAIFEAVHGSAPDIAGKDIANPTALLQS
                     AVLMLRHMDEDAAADKIQSALEKVYAEKKTLTRDVGGTAGTKAFADAVIAAL"
     gene            509..1264
                     /locus_tag="VFA02_06275"
     CDS             509..1264
                     /locus_tag="VFA02_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09494.1"
                     /translation="MKRSVLAASVVCTLLLSAYGCRKPSGGEQTAHYTQIDWSTAGTI
                     SGTIHFSGKMPAPVQIDMAQDPVCSLSPANYSEQYVGKDGGLQNVFIYVKDGLGNKIY
                     AAPVTPVVMDQKGCRYTPHVIGVMVGQPVRFTNSDPTMHNVHMLPTVDSNQATDISQG
                     PNGAPETRVFKKPELMIPVRCNNHPWMQAFINVVDNPFYAVSDAEGHFEIKGLPPGTY
                     TVVADHEVLGQQTATVTVAAKQTAKQDFTFAAK"
     gene            1288..1908
                     /locus_tag="VFA02_06280"
     CDS             1288..1908
                     /locus_tag="VFA02_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09495.1"
                     /translation="MAMGTAAVSSTAKRTTSVLLWLALIAQIAWMASQELRSHVLWTA
                     MWYPLTFVIVCLAMACTNGHVRWIAALLRILIALAFLQAVGDRFGLFGGPGTPGVAWG
                     DFAHFIAYTGKVNSFMPHAVIPALAVTATLAEVLFGLALLLGICIRYATIGSALLLTA
                     FATAMTISGLSQFRYSVYLMAAGALYLATVNASFLSVDAIAGRQGK"
     gene            2025..3089
                     /gene="asd"
                     /locus_tag="VFA02_06285"
     CDS             2025..3089
                     /gene="asd"
                     /locus_tag="VFA02_06285"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZU09496.1"
                     /translation="MQRYQIGILGATGMVGQRFIQLLERHPWFEVVWLAASDKSSGKR
                     YGDAVKWKLDTPLPRHIAEMQVSPAAPEGAPKIIFAALDSDIARELEPKFAAAGCAVI
                     SNSSAFRMQEDVPLVIPEVNADHLPLLENQRWRKQSGGYIVTNPNCSAIGLVLALKPL
                     EERFGIEAIFVSTMQAVSGAGYPGVASLDILGNVVPFIKNEEEKMQEETLKLLGSLKG
                     TRVQPLDAKISAHCNRVAVEDGHTENVSIKLRKPATREQILSAWSEFQPLAGQHLPTA
                     PAQPVEFIAADDRPQPRLDRMRGNGMAAVVGRLRPCTLFDWKFTVLSHNTIRGAAGAA
                     LLNAELLATLGKLEPVAVPA"
     gene            3086..4555
                     /gene="lysC"
                     /locus_tag="VFA02_06290"
     CDS             3086..4555
                     /gene="lysC"
                     /locus_tag="VFA02_06290"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HZU09497.1"
                     /translation="MKKLVVMKFGGTSVEDAVAINRTATIVAGRRNQGVQPIVVVSAM
                     AKVTDQLLAAANAASRGDRNGALAITARLRSRHKEVACRLTSQAEGRAGNGCEDFTGP
                     QHAASQQWIDAEFDALDEILRGLSAVGELTPRISDMIVSYGERLSSRMIAEAFTQRGL
                     DGVHVDARNCIITDAQHGKAIPQDTPIAERLKEYVLPHAYDGRTVVMGGFIGSTEKGV
                     TTTLGRGGSDFTAALVGGGIHAEAIEIWTDVNGIMTADPRVCPDALRVKTISFEEAAE
                     LAYFGAKVLHPATILPAVQKNIPVLVLNSRNPENEGTRIISLAPKCRSPFKSIAVKKK
                     VTIIDVVASRMLMTHGYLKAIFDVFDKHKCPVDLVSTSEVSVSLTVDTTEKIPEIAAD
                     LSKLADVKYEGRKALVCMVGENIRGQNGIAARVFGAIRHINIRMISQGASEINMSFMI
                     EEDDADEAVRSLHAEFFEDPDPEIFDVEARKGATAATAR"
     gene            4558..5238
                     /locus_tag="VFA02_06295"
     CDS             4558..5238
                     /locus_tag="VFA02_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZU09498.1"
                     /translation="MLFLVLGRGKTGRVVADVAAEHGHSVRVVGEEENRNAMALTAPF
                     LAGFDAVIDFTTPEAAVSNMRACLANGARMVVGTTGWYQHLEDMRLLAARRNAGFLYG
                     TNFSLGVQAVIRLARELAVSLPKYQFRITETHHVQKKDAPSGTALSLKQALQSANPAL
                     QVEIVSKREGDVSGIHVIEARSKDEVIELKHESFSRRAFAEGAVRAAEWIAGKTGVWD
                     FSEIAGQL"
     gene            complement(5235..5468)
                     /locus_tag="VFA02_06300"
     CDS             complement(5235..5468)
                     /locus_tag="VFA02_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZU09499.1"
                     /translation="MSQKRVILYSQPGCPPCYAAKHFLTSREVPFEYKDVTADPSALR
                     ELVKLNSRSTPTIVVGDEVMIGFDPGRLEQLLG"
     gene            complement(5605..7572)
                     /gene="acs"
                     /locus_tag="VFA02_06305"
     CDS             complement(5605..7572)
                     /gene="acs"
                     /locus_tag="VFA02_06305"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZU09500.1"
                     /translation="MASTSTSLNPHLDSILREHRVFPPPAEFAAKARIKNIFEYEQMY
                     RRSVEDPEGFWAEAARDLHWFKPWNKVLEWNQPWAKWFAGGKTNLCYNCVDRHALGGK
                     RDKVAILWEGEPGEVRRLTFGELHAEVQRFANVLKGLGIKKGDRVAVYMGMTPELAIA
                     ILACARIGAVHSVIFGGFAAHAIVDRVNDAQCVAILTQDTAYRRGNEVKLKATVDEAL
                     PQCPTVKNVVVYRRSGSEVKMQAGRDHWWHELMGKAAPECPAEELDSEDPLYILYTSG
                     TTGKPKGLVHTTGGYAVQTYLTSKYVFDLRDDDIYWCSADIGWVTGHSYVVYGILQNG
                     VTTLMYEGAPNFPAADRFWKIIDDHKVTVFYTAPTAIRAFIKWGDEYVQKYKLDSLRL
                     LGTVGEPINPEAWMWYREVIGKGRCPIVDTWWQTETGSIMIAPIPGAVPTKPGSATRP
                     FFGIVPEVVTKEGQPVPAGHGGLLVIRKPWPSMARTIFNDPERYVKAYWSEIPGSYFT
                     GDGARVDEDGCFWLMGRVDDVLNVSGHRLGTMEVESALVAHPKVAEAAVVGRPDELKG
                     QAVAAFVTLESGHQPSRELKEELRAWVAKEIGALAKPDDIRFTDQLPKTRSGKIMRRL
                     LRELATSGEIKGDTTTLEDFSIIAKLREQDE"
BASE COUNT         1652 a         2223 c         2130 g         1645 t
ORIGIN      
        1 gatggcctgt ttctgaagtg cgcgcgcgaa gtgggcaagg gctttcccga gatcacctat
       61 ggcgagcaca tcattgacaa cacctgtatg caattggtgc tgaacccata ccagtatgat
      121 gtgctgctgc ttgaaaacct ctacggtgat atcgtcagcg atctttgcgc tgcctttgtt
      181 ggcggacttg ggctggtccc aggagcaaat cttggcaccg aagcggcgat ttttgaggcc
      241 gtccacggtt cagcgccgga cattgccgga aaagacatcg ccaatccgac agcgctgctg
      301 caatcggcgg ttctgatgct gcggcatatg gatgaagatg ctgccgcaga caaaatccag
      361 tctgcgctcg aaaaggtcta tgcggaaaaa aagacgctca cgcgcgatgt gggcggtact
      421 gctggtacaa aggcctttgc cgacgcggtg attgctgcat tgtaaatatt tccttgggca
      481 ctactgagtt tcttatctgg agaagcacat gaaacgttct gttctcgctg cgtcggttgt
      541 ttgcactctt ctgctgtcgg cttacggatg cagaaaacca tccggcggcg aacagacggc
      601 ccattatacc cagattgact ggagcacggc aggcaccatt tccgggacca tccatttctc
      661 cgggaaaatg cctgctccgg tgcagattga tatggcgcag gaccctgtgt gcagcctcag
      721 tcctgcaaat tacagcgagc agtacgtggg taaagatggc ggtctgcaga atgtctttat
      781 ttatgtgaag gacggcctgg ggaacaaaat ctatgctgct ccggtcaccc ctgtggttat
      841 ggaccagaaa ggctgccgct ataccccgca cgtcatcgga gtgatggtgg gccagccggt
      901 tcgctttacc aacagcgacc cgacgatgca caacgtccac atgctgccca ctgtggattc
      961 aaaccaggcg acagacatct cgcaggggcc gaatggagcg ccggagacgc gcgtctttaa
     1021 aaagccggag ctgatgattc cggtacgctg caataaccat ccctggatgc aggcatttat
     1081 caatgttgtg gacaatccct tctacgcggt cagcgatgcg gaaggtcatt ttgaaatcaa
     1141 aggactgccg ccgggcacat ataccgtcgt tgccgatcat gaggtcctcg gccagcagac
     1201 agcaacagtc acagtcgccg caaagcagac cgcgaagcag gactttacct tcgcggcaaa
     1261 ataaaagcgt tcacccacta tagactggtg gccatgggaa ccgctgccgt ttcatccaca
     1321 gctaaacgga ccacgtcagt cttgctgtgg cttgctttga tcgcgcagat cgcatggatg
     1381 gcttcgcagg agctccgttc gcatgtcctg tggaccgcga tgtggtatcc gcttacattc
     1441 gtgatagttt gcctcgcgat ggcctgcacg aatggacatg ttcgatggat tgcggcgcta
     1501 ttgcgaattc tcatcgcgct cgcattcctt caggccgtgg gcgatcgctt cggccttttt
     1561 ggtgggccgg gtactccggg cgtagcatgg ggagatttcg cacatttcat cgcttacaca
     1621 ggcaaagtca attcattcat gcctcatgca gtcattcccg cactggcggt gacggcaaca
     1681 ttggctgagg ttctctttgg attggccctg ctgctcggta tctgtatccg gtatgccaca
     1741 attggctctg ctctgcttct caccgccttt gccacggcaa tgacgatctc aggtctgtca
     1801 cagttcaggt attccgttta cctgatggcc gctggagcgc tttatctggc aaccgtcaac
     1861 gcatcgttcc tcagtgtgga tgccatcgca gggcggcaag gaaaatagcc ggtcggccat
     1921 tcatctgttc gtaagaaaca gttacactat tgagtacagc gaacaatcca gccccatcag
     1981 gacgtgggca gcggtgtaca tcctcgctga atggaaagag gcacatgcag cggtatcaga
     2041 ttggcattct gggcgcgacc ggaatggtcg gccagcgttt tattcagctt cttgagcgcc
     2101 acccctggtt tgaggtggta tggcttgcgg ccagcgacaa gtcctctggc aagcgctatg
     2161 gcgacgccgt aaagtggaag ctcgacactc cgttgcccag gcacatcgct gagatgcagg
     2221 tctcacctgc cgccccggaa ggcgccccta agatcatctt tgctgcgctt gactctgaca
     2281 tcgcccgcga gctggagcca aaatttgccg ctgccggttg tgccgtcatc tcgaattcca
     2341 gcgccttccg catgcaggaa gatgtgccgc tcgtcattcc ggaagtgaat gctgaccatc
     2401 tgcctctgct cgaaaaccag agatggcgca aacagtccgg cggatacatc gtcactaacc
     2461 cgaactgctc ggcaattgga ctggtgctcg ccctcaagcc gctggaagaa cgcttcggaa
     2521 ttgaggcgat tttcgtcagc acgatgcagg ccgtcagcgg cgccggatat cctggtgtgg
     2581 cctcactcga tattttgggt aatgtcgtcc ccttcatcaa aaacgaagaa gaaaagatgc
     2641 aggaagaaac gttgaagctg ctgggctctc tgaaaggcac tcgtgtgcag cctcttgacg
     2701 caaaaatctc cgcgcattgc aaccgcgtcg ccgtggaaga cggacacacc gaaaacgtga
     2761 gcatcaagct gcgcaaacct gctacacgcg aacagattct ttctgcgtgg agcgaatttc
     2821 agccgctcgc cggacaacac ctgcccaccg ctcctgctca gcccgtcgag ttcatcgcag
     2881 cggatgaccg cccgcagccg aggcttgacc gcatgcgcgg aaatggcatg gccgcggtcg
     2941 tgggacgcct ccgcccctgc actctttttg actggaagtt cactgtcctc tcgcataaca
     3001 cgattcgcgg agcagcgggc gctgctctgc tgaacgcaga attgctggca acgctgggca
     3061 aacttgaacc tgtggcggtc ccggcatgaa gaagctcgtt gtcatgaagt tcggcgggac
     3121 ctccgtcgaa gatgccgttg ccatcaaccg cactgcaacc atcgtcgccg gaaggcgcaa
     3181 ccagggcgtg cagcccattg tcgttgtcag cgcgatggcg aaagtcacgg accagttgct
     3241 cgccgctgcc aatgctgcct cgcgcggaga ccgcaacggc gcgcttgcca ttaccgcccg
     3301 tctgcgtagc cggcataagg aagtagcatg ccgtctcact tctcaggctg aaggcagggc
     3361 aggaaacggc tgtgaggact ttaccgggcc gcagcacgcc gcatctcagc agtggattga
     3421 cgccgagttc gacgcgctcg atgaaattct gcgcggactg tcggcggtcg gcgagctgac
     3481 gccgcgcatc agcgacatga ttgtctccta tggcgagcgc ctttccagcc gcatgatcgc
     3541 cgaggctttt actcagcgcg gactcgacgg cgtccacgta gatgcgcgca actgcatcat
     3601 cactgacgcg cagcacggta aggccatccc gcaggacacc ccgattgccg aaaggctgaa
     3661 ggagtacgtt ctgccccatg cctatgatgg caggaccgtc gtgatgggcg gcttcatcgg
     3721 ctcgacggag aagggcgtta ccacaaccct tggccgtggc ggttccgact tcaccgctgc
     3781 gctcgtcggc ggtggaatcc acgccgaagc catcgaaatc tggacagacg tgaacggtat
     3841 tatgacggcc gatccgcgtg tctgcccgga tgcgctgcga gtcaaaacca tcagctttga
     3901 agaagctgcc gagcttgcct atttcggcgc gaaagtgctg catccggcca ccatcctccc
     3961 ggcagtccag aagaacattc cggtgctggt gctcaattcg cgtaatcctg aaaatgaagg
     4021 aacgcgcatc atctcgcttg cgccaaaatg ccgcagcccg ttcaaatcca ttgccgtcaa
     4081 gaagaaggtc acgattattg atgttgtcgc cagccgcatg ctgatgacgc acggctatct
     4141 gaaggccatc ttcgatgtct tcgacaagca caaatgccca gtggacctgg tttctacctc
     4201 ggaagttagc gtctcgctga ccgtggacac tacagagaaa atccccgaga ttgccgccga
     4261 cctgagcaaa ctggccgatg tgaagtatga aggccgcaag gcgctggttt gcatggtagg
     4321 cgaaaatatc cgcggccaga acggcatcgc cgcgcgtgtc ttcggggcca tccgccacat
     4381 caacatacgc atgatctccc agggcgcttc ggaaatcaat atgagcttca tgatcgaaga
     4441 agacgatgcc gacgaggccg tgcgctcact tcatgcagag tttttcgaag accccgatcc
     4501 ggagatcttc gacgtggaag cacgtaaagg cgcaacagca gcaactgcga ggtgaccatg
     4561 ctttttcttg tgctgggccg cggaaaaacc ggacgcgttg tggccgacgt ggcggctgaa
     4621 cacggacaca gcgttcgcgt cgtcggagaa gaagaaaacc gcaatgcaat ggcgttgacc
     4681 gcaccttttc tggctggctt tgatgcagtc atagacttca ccacgccgga ggccgccgtt
     4741 tccaatatgc gggcctgcct tgcgaatggc gcgcgcatgg tggtcggcac caccgggtgg
     4801 tatcagcacc tggaggacat gcgcttactg gcagcgcgca ggaacgccgg ctttctttac
     4861 ggcacaaatt tttcactcgg agtgcaggcc gtcattcgtc tggcacggga gcttgcagtt
     4921 tctctgccga agtaccagtt caggattaca gaaacacatc acgtccagaa aaaagacgct
     4981 ccctcaggga cggcgctttc gcttaaacag gccctgcaat ccgcaaatcc cgctttgcag
     5041 gttgaaatcg tctcaaagcg cgaaggcgat gtctccggca tacatgtgat tgaggcccgc
     5101 tcaaaagacg aggtcatcga attaaagcac gagtcctttt cgcggcgggc tttcgcagaa
     5161 ggcgctgtcc gcgccgcaga atggatagcg ggcaaaaccg gcgtctggga cttcagcgag
     5221 attgcaggcc agctttaacc gagcaactgc tccagacggc cgggatcgaa gccgatcatg
     5281 acctcgtccc ctacaacaat cgtcggagta gagcggctgt tcagtttcac cagttcgcgc
     5341 aatgcggaag ggtctgccgt tacgtcctta tattcaaagg ggacttcgcg ggaagtaaga
     5401 aaatgcttgg cagcatagca gggagggcat cccggctgac tgtagagaat gacacgtttc
     5461 tgagacataa agctattcaa aaagatgcag gaataccggg ccaggattca gggcacattt
     5521 tcacgtgccc ttgtcttggt aaacactgat ttgtccactt gcaagccaag ggtttacaag
     5581 tagtcaatca tcacgcttga aaccttactc atcctgttcg cgtaattttg caatgatgct
     5641 gaagtcttca agcgtcgttg tgtcgccttt gatttcaccg ctcgttgcca gctcgcgcag
     5701 cagacgccgc atgatcttgc ccgagcgcgt cttgggcagc tggtcggtaa aacggatgtc
     5761 gtccggtttg gcgagtgcgc cgatttcctt tgctacccag gcgcgcagtt cttccttcag
     5821 ctccctggag ggctgatggc cgctttcgag cgtcacaaat gcggcaacgg cctgtccttt
     5881 caattcatcc ggacggccta cgactgctgc ctcggcgact ttcggatgcg ccaccagcgc
     5941 agactcgact tccatcgtac ccaaacggtg tccgctgacg ttcagaacgt cgtccacgcg
     6001 gcccatcagc cagaagcagc cgtcttcgtc cacacgcgcg ccgtctccgg taaagtacga
     6061 gccgggaatc tctgaccagt aggcttttac atagcgctcg ggatcattga aaatggtccg
     6121 cgccatggaa ggccacggct tgcgaatcac gagcagtccg ccatggcctg cgggaacagg
     6181 ctggccctct tttgtaacta cttcaggcac aattccaaag aagggccgag tagcagagcc
     6241 tggtttggtg ggcacggcac cgggaatggg tgcaatcatg atgctgcccg tctctgtctg
     6301 ccaccatgtg tccacaatag gacagcgtcc tttgccaatc acttcgcggt accacatcca
     6361 ggcttccgga ttgatcggtt cgcccaccgt gcccagcagg cgcagcgagt caagcttgta
     6421 cttctgcacg tattcatcgc cccacttgat gaacgcgcga atggccgtcg gcgcggtgta
     6481 gaacaccgtg accttatggt catcaataat cttccagaag cggtccgcag cgggaaaatt
     6541 cggtgcgcct tcatacatca gcgtggtcac gccgttctgc agaattccat acacgacata
     6601 ggaatgcccc gtgacccagc cgatatcagc cgaacaccag taaatgtcat cgtcgcgcag
     6661 gtcaaagaca tatttgctgg tcaggtatgt ctgcaccgca tagccgcctg tcgtatggac
     6721 caggcctttt ggctttcctg tcgtgccgga tgtgtaaaga atgtagagcg ggtcttcaga
     6781 gtcaagttct tccgcaggac attccggtgc ggccttcccc atcaattcat gccaccagtg
     6841 gtcgcggcct gcctgcatct tcacctcaga gccagagcgc ctgtagacga cgacattctt
     6901 cactgtcggg cactgcggca gcgcctcatc taccgtggcc ttcagcttca cttcattgcc
     6961 gcggcgatac gctgtgtcct gcgtaagaat agcaacgcac tgcgcgtcat ttacgcggtc
     7021 cacaatagca tgggcagcaa atccgccaaa gatgaccgaa tgcacggccc cgatgcgcgc
     7081 acaggccaga atcgcaatcg ccagctccgg cgtcatgccc atgtagacgg ccacgcggtc
     7141 gcccttcttg attcccagtc ccttcagcac attggcaaat cgctgcacct cagcatgaag
     7201 ctcgccgaaa gtcagcctgc gaacttcacc cggctcgccc tcccacagaa tggcgacttt
     7261 gtcgcgcttg ccgccgagtg catgacggtc cacgcagttg tagcagagat tggttttacc
     7321 accggcaaac cactttgccc acggctggtt ccattccagc accttgttcc atggcttgaa
     7381 ccagtgcaga tcgcgcgctg cttctgccca gaacccctcc ggatcttcca ccgaacggcg
     7441 gtacatctgc tcatattcaa agatgttttt gatgcgggcc ttcgccgcaa attccgcagg
     7501 tggaggaaaa acgcgatgct cgcgcaggat ggaatcaagg tgcggattca ggctggttga
     7561 cgtagacgcc atgcgggctg aaatacctct ttctgacgaa aattcttcag catccacgtt
     7621 aacggttgca agcaacgagt ggcaagatgc
//
LOCUS       DASRBV010000164         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_364315, whole genome shotgun sequence.
ACCESSION   DASRBV010000164 DASRBV010000000
VERSION     DASRBV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364315"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1013)
                     /locus_tag="VFA02_06310"
     CDS             complement(111..1013)
                     /locus_tag="VFA02_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09501.1"
                     /translation="MIFFIALGVCLVGVAIALNIGWIILNWRRDWPMLVGIPFFLLLI
                     AGVVLNTIFLVREVRRNERHDSFINAVTHELKTPIASIRLYLETLQRRALSEEQRQHF
                     YSIMLADSERLLATVEQVLKAGEVGQRARNQVRTETDMAALTQKCIETTILQHHLAPD
                     TITFSESNPDQPMLVRGNPDELETAVMNILNNAVKYSPNGLRARVQLALEGDAWVVLS
                     VSDEGIGIAPAHLKRIFKRFYRVPARNVLRTKGTGLGLFLVRSIARQHGGDAIAESAG
                     EGRGTTIRLQLPRLLDSTARSPME"
     gene            complement(1102..1896)
                     /locus_tag="VFA02_06315"
     CDS             complement(1102..1896)
                     /locus_tag="VFA02_06315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZU09502.1"
                     /translation="MIAFHLGAIAALFFFSWSALAVSIVLYMFAVNVGIGMGYHRLLT
                     HRGYKTPKWVEYCIAVCGTLALEGGPIFWVATHRLHHQLSDHTGDPHTPTEGGWWAHL
                     GWILSGETMHSQTAIHARYAPDLAKDRFYVWLSEYHWVPLTVIGLLLFAIGGWSWMLW
                     GVFLRVIVGLHATWLVNSATHMWGSRRFPTRDDSRNNWWVALLTGGEGWHNNHHAHPV
                     SARHGLKWYEFDPNYYGIWLLKKVGLARDVKIAQYDPKNPRPAGAA"
BASE COUNT          468 a          606 c          636 g          484 t
ORIGIN      
        1 tcatgacctt cagcctgaag attaaagaag aggccctgcg ccagatgggt ctcatcttca
       61 accacgagaa tgcgtgctga cctggaagac atatagccac tttatcgcgt tcattccatc
      121 ggagaacgtg cggtggagtc cagcaggcgc ggtaattgca ggcggatggt ggtgccgcgt
      181 ccttcgccgg cgctctccgc gattgcgtct cctccatgtt ggcgggcaat ggagcggaca
      241 agaaatagcc ccagtccagt gcctttggtg cgcagaacat tccgcgccgg aacgcggtag
      301 aaccgcttga agatgcgctt cagatgcgcc ggagcaatgc caatgccctc atctgagacg
      361 ctcagtacca cccatgcgtc gccttccagg gcgagctgca cgcgcgcgcg caggccattt
      421 ggagaatact tcaccgcatt gttcaggatg ttcatgacag cggtctccag ctcatccgga
      481 ttgccgcgca cgagcatggg ctgatctgga ttgctttcag aaaaagtgat tgtgtccggc
      541 gcgaggtggt gctgcaggat ggtcgtctcg atgcactttt gggtgagtgc ggccatgtcg
      601 gtttctgtcc gtacctggtt gcgcgcccgc tggcctactt cgcctgcctt gagtacttgc
      661 tcgacagtgg ccagaaggcg ctcactgtca gccagcatga tgctgtagaa atgctgtcgc
      721 tgctcttcgc tcagggcgcg gcgctggagc gtttccagat acagtcggat ggaagcgata
      781 ggcgttttca gttcgtgcgt aaccgcgttg atgaagctgt catgccgctc attccgccgg
      841 acttcacgca ccagaaaaat cgtattcagc acaacacctg caattaacag aagaaagaac
      901 gggattccga ccagcatcgg ccagtcgcgg cgccagttca ggatgatcca gccaatattg
      961 agcgcaatag caaccccgac caggcacaca cccagcgcaa tgaaaaagat catggctggg
     1021 cggcgtcggg tgatgcgcat cctggtatgg tttatagcag gcaaatcccg catcctgaaa
     1081 gggatatggg gacgaatgtt tctatgctgc gccggctggc ctcgggtttt tgggatcgta
     1141 ctgcgcaatc ttcacatcgc gtgcaaggcc cactttttta agcagccaga tgccgtaata
     1201 attcgggtcg aattcatacc atttcagtcc gtggcgcgca gagacgggat gagcgtggtg
     1261 gttgttgtgc cagccttcgc ctccggtcag cagggccacc caccagttgt tgcgcgaatc
     1321 atcgcgggtg gggaagcggc gggaacccca catatgcgtg gcggaattga caagccatgt
     1381 agcgtgaagt ccaacaataa ctcgaaggaa gacgccccag agcatccagc tccatcctcc
     1441 tatggcgaac agcagcagtc caatcactgt tagtggtacc cagtgatatt cactcagcca
     1501 gacatagaag cggtctttgg caaggtcggg cgcatagcgt gcatggatgg ccgtttgtga
     1561 gtgcatagtc tcgccgctca gaatccagcc caggtgcgcc caccatccgc cttctgtcgg
     1621 agtatggggg tcgcccgtat ggtcagagag ttggtgatgc agcctgtgcg tcgctaccca
     1681 gaagataggg ccgccctcga gggccagagt gccgcatacc gcgatgcagt actccaccca
     1741 tttcggcgtc ttatatccgc ggtgcgtcag caggcgatga tagcccattc cgatgcccac
     1801 gttgaccgca aacatgtaca gcacgataga aactgcgagc gcactccagg aaaagaaaaa
     1861 tagtgcggcg atggcgccaa ggtggaaggc gatcatgaac gatgttgtga gccagtttgt
     1921 ccttccgccc tgcgcggcac gccccagcgt gggtacttcc ggcttcgcgg tgtagcgctt
     1981 gagtggcact tcagcttcag caacgacggg ttcttccagt aattcaacag gcattcggaa
     2041 ctcagaatcc ttgaggaaat atcttcctca ttccaacgta tcagcgtgcc ggcatggtgc
     2101 atgtaagact tttgtaatgt cgtcgtgata gtttgctatc gtgccatgaa acgaaatttt
     2161 ctgctgcttc tgttgtgttc tgcctgtgcc gcac
//
LOCUS       DASRBV010000165         6882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_313976, whole genome shotgun sequence.
ACCESSION   DASRBV010000165 DASRBV010000000
VERSION     DASRBV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313976"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFA02_06320"
     CDS             complement(<1..1025)
                     /locus_tag="VFA02_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09503.1"
                     /translation="MKKTIICDLGLVLVCAIASAQIGNSTRQQIEMHSQKAHAFLQEK
                     RPDLAIPEFQSIISLDPKNVDAQGNLGVLLFFQGNYAEAAPHLRSAVQLHPGLWKIQA
                     LLGLAEARLNDSSAGRADLEAAFPHLEDEKFKNDVGRALIDNYTAGGDLEKAAAAVSI
                     LLTSQPTDPSLLYMSYRINSDIAGKSMLTLALTAPYSAEMHQVMARELARHGDETAAI
                     ANYREAIKINPNLPGLHTELGDVLYNSLEENNKNAAEAEFKAALAVNSNDEHAELMLG
                     EIAARRGDMQAAYEHESRALSLQPNDGDACTELAKTLLLMNERDKAQQMLERAVQIDP
                     SDYVAHYR"
     gene            complement(1114..4464)
                     /locus_tag="VFA02_06325"
     CDS             complement(1114..4464)
                     /locus_tag="VFA02_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09504.1"
                     /translation="MAGVLSTAIGFAQSTNAGDIRGTVTDPSGAVVPDVKVTVLNVDT
                     GVSKEFVTNSDGLYDTSSIVAGNYKVTFSKDGFDELVRGPITLQVGFTTVNAQLKIGS
                     TTQSVVVNSDVPLLKTESGEQVTTLDAKSMAQMPQVASASGVSWENFTILLPGASGTP
                     NGSQGAANPGQEVAINGNLPYSNILADGASTTLSHSQNANPAIFETVSELQVNTSTFS
                     AQYGIGGIIFNQISKGGTNQFHGSAYDYFQNDALNAANYGFGNKVTVPFLRYNEFGGS
                     ISGPILKRKMFFFFNYDQIVDHGSASNSFNTVPTTAVMSGDFTGQPLIYDPTTQTIGH
                     DAKGNPYPIRKSFLEEYGSNAIPQAMFDKVAAAFQQFYPTPSNHIPGGKFVPGSLNSV
                     GLLQNNFYSSLPQSTPYRKYFGRLDYDITPNNRLTMSDTQSDTPVIYPNSVTACPIGC
                     QSGDVDNNNAQITDVWNISSTTINEARLGYTWQGNFFADLSLGKGYPGKLGWQFAKAD
                     TIPAIQFGPPYPYAWIQPSSNAVYKEHVFDPSDVVTMIRGKHILHFGGEVLVYRDNST
                     AWGNTNAGTMNFSGQYTQQWTLDANGVAQPNTSTGLNYADFLLGQVNSWNAQVSPEYG
                     GRLKDAHIFIQDDYKVRPNLTLNIGLRYQIRHGWNEVKNNMAIFDPEVINPATNTPGA
                     FWYGSTRAHGRGALQADVYDTVLPRVGFSWLPAPNTTIRGGFGIYAYNWSLDTYGAGM
                     GNEVRSSGSVTDQTNGIIPVVLLGGSGSNLPYVAASTDPAAYNGQDVSYNQFHTPVPK
                     IYQWTLAVQRSLGTNMVGEIAYVASHGQNLAFPVDVNQVPESKFSSNDSGDRPYPIYG
                     SISGSTNNAISNYNSLQASITKRFTSGLSFNFNYVWSHMLDDQDSSGWGSRAGDQPYQ
                     NAYKPSANYGSSNFDVRNAFKGYAVYELPFGIGKTFLNHNRLMDAAIGGWQLAGTLVL
                     STGNPFTVTTSGPSYALAGSIYPNATGISPIPKHRTIYEWYNPAAFAQPAAGQWGNVR
                     RNSLYGPGLNQVNLSLGKTFSIWEEVKLQIRADATNAFNHPSFGVPQNALSGAAPGQP
                     FPENLFSQQINSTTVGGRTMQLSTHLTF"
     gene            4749..6155
                     /locus_tag="VFA02_06330"
     CDS             4749..6155
                     /locus_tag="VFA02_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HZU09505.1"
                     /translation="MMKSTSRRTFLKLSAMTAASTALPSFATANAGPVKVWRTSAEER
                     WQTLEALAWEPFRGTSPVSIRLIPARKYQQVLGFGAALTDASCYLLEQLDAGKRKTIL
                     EDCFGPSGLRLSVARTTIGSSDYSLSAYSYDDTQQPDPGLKHFSIDHDRKYILPLLRE
                     VQNVNPEMFYFSSPWSPPGWMKAGGSLLGGSMRSHYFDAYAQYFVKFLQGYAANGVKI
                     EAVTVQNEVDTDQDGRMPQALWGQEYEMNFVKDHLGPALKQAGLDTKIWILDHNYNLW
                     GRAVDEISDPDVYQYVDGVAWHGYYGDPSAMTRVHDMFPEKNAYWTEGGPDYKSPDYA
                     TDWTKWSNTFTGILRNWARCIVSWNLALDENGKPNIGPFDCGGLVTVHSGTHEITRSG
                     QFWAFAHYSKVIHRGAHIFASSGDLQNISHVAAENPDGSRVLVLTNTEREQTVQVVLE
                     EQALSLRLPPDSVTTLVW"
     gene            complement(6139..>6882)
                     /locus_tag="VFA02_06335"
     CDS             complement(6139..>6882)
                     /locus_tag="VFA02_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU09506.1"
                     /translation="HRHRPDLIEQYPRMLISRGAEGIIAIDTHLDHKLAVPAVAVAGH
                     RKISGVTNVVLDHNTAAELTMRHLRDLGHRSIAFMRGQPYSSDSEVRWQGIVKAAREL
                     GIAIRPELTVELTEDLTSPELGYPVVQKLLAHHRRFTAIVCFNDMAAIGAIRALHDAQ
                     LRVPQDISVIGFDDVPQAAFQTPSLTTVRQPLHEMGKLSAQLLLQRLRSQGAIPAEVA
                     VKPQLIVRESTGPVRENAASKVRRLTRQG"
BASE COUNT         1672 a         1739 c         1775 g         1696 t
ORIGIN      
        1 cgataatgcg ccacatagtc tgaagggtca atctgaacgg cacgttcaag catctgctgc
       61 gccttatcgc gctcgttcat cagaagaagt gttttggcca attccgtgca ggcgtcaccg
      121 tcatttggct gcaaggacag agcgcgcgat tcatgttcat atgcggcttg catgtctccg
      181 cgtcgtgctg caatttcacc cagcatgagc tcggcatgtt cgtcatttga atttacagcg
      241 agcgcagcct tgaattcagc ctctgctgcg tttttattgt tctcctcaag agagttgtac
      301 aggacgtctc ccagctcggt atgcagcccc ggcaggttgg gattaatttt gatggcctcg
      361 cgatagtttg caatggcggc ggtttcatct ccatgccgcg ccagctcgcg ggccattacc
      421 tgatgcatct cggcactata aggagcggtc aacgccagcg tgagcatgga tttgcccgcg
      481 atgtcagagt tgatccgata gctcatgtag agcaaagaag ggtctgttgg ctgggaggtc
      541 agcagtatgg aaaccgcggc tgcggctttt tccaggtcgc cgcctgccgt gtaattgtct
      601 atcagcgcgc ggcccacgtc attcttgaat ttttcatctt ccaggtgggg aaatgcagct
      661 tcaagatcgg cgcggccggc actggagtca ttcagtcttg cttccgcaag gccaagcagt
      721 gcttgtattt tccatagccc gggatgcaac tggacggcag agcggaggtg cggtgccgct
      781 tctgcatagt tcccctgaaa aaacagcagg acaccgagat ttccctgcgc atctacattc
      841 tttgggtcga gagaaataat ggactgaaac tcaggaatag ccagatcggg gcgcttttcc
      901 tgcaaaaagg cgtgggcctt ctgactgtgc atttcgattt gctgccgagt cgagttacca
      961 atctgggccg atgcgatagc gcaaactaaa accaggccca gatcgcaaat gatagttttc
     1021 ttcatataac acagggtact ttgtcctgca atttgtctta aaataaaaag agacggcatc
     1081 aaaagccgcc tctttgagtg cataatcttt aactcagaat gtcagatgcg tactgagctg
     1141 cattgtgcgt cctcccaccg tagtcgaatt gatctgctga gagaacaggt tttcaggaaa
     1201 tggctgaccc ggtgccgcgc ctgataaagc attttgcggc acaccgaaac tgggatgatt
     1261 aaaagcgttc gtcgcatcgg cccgaatctg aagcttgact tcttcccaga tggaaaaagt
     1321 cttgcccagt gaaagattaa cctggttcaa accaggaccg tagaggctgt ttcgccttac
     1381 attaccccac tgtccggccg caggctgagc gaaagctgcc ggattgtacc actcataaat
     1441 ggtccggtgc tttgggatcg ggctgatgcc tgttgcattg ggatatatcg aaccagccag
     1501 cgcatatgaa gggccgctcg tggtcactgt aaatggattt ccggtagaca gaacgagcgt
     1561 gcccgcaagc tgccagccac caatagccgc atccatcaag cggttatggt ttagaaatgt
     1621 tttacctata ccaaatggaa gttcgtatac tgcatatccc ttgaaagcat tccgtacatc
     1681 gaagttggaa cttccatagt tggcggaagg tttataagca ttctgatagg gctgatcgcc
     1741 agcgcggctt ccccatccag atgagtcctg gtcatccaac atatgtgacc acacataatt
     1801 aaagttaaag ctcagaccag aagtgaagcg cttggtaata gatgcctgaa gcgaattgta
     1861 gttggaaatg gcgttgtttg tacttcccga aatacttcca taaatgggat acgggcgatc
     1921 accgctatcg tttgatgaaa attttgattc aggcacctgg ttcacatcta ccggaaacgc
     1981 cagattctgg ccgtggcttg caacataggc gatttcgcct accatgtttg tacccaggga
     2041 acgctgaact gccaaggtcc actgataaat tttgggcact ggggtatgaa actgattgta
     2101 actaacatct tgcccattgt aggcagcagg atcagttgaa gcagccacat aaggcaggtt
     2161 tgatccacta ccaccaagca gaacaacggg aatgattccg tttgtctgat ccgttacact
     2221 tcccgaagac ctgacttcat tgcccattcc agcgccataa gtgtcaaggc tccagttata
     2281 ggcgtaaatt ccaaaaccgc cgcgaatggt tgtgttcgga gcaggcagcc aggaaaatcc
     2341 tacacgcggc aaaaccgtat cgtagacatc ggcctgaagg gcaccacgcc cgtgggccct
     2401 ggtagagcca taccagaaag caccgggagt attggtagct ggattgatta cttccggatc
     2461 aaagatcgcc atgttgttct tgacctcgtt ccagccatgg cgtatctggt aacgcaaccc
     2521 gatattcaaa gtcagatttg gacgtacttt atagtcatct tgaatgaata tatgagcatc
     2581 ttttaaacgg ccaccgtatt ccggagaaac ctgagcattc caggaattta cctgaccgag
     2641 caggaaatcc gcatagttca gaccagtcga agtgttaggc tgtgccacac cgttggcatc
     2701 gagcgtccat tgctgcgtat attgccctga aaaattcatt gtccctgcat tggtgttgcc
     2761 ccaggctgtg gagttgtcgc ggtagaccag cacttctcca ccgaaatgga ggatgtgctt
     2821 gccgcggatc atagtcacaa cgtctgatgg gtcaaagacg tgctccttgt agactgcatt
     2881 tgaactgggt tggatccagg catatggata cggtggccca aactggattg caggaatcgt
     2941 atccgccttg gcgaattgcc agccaagttt ccccggatac cctttaccta aggacaaatc
     3001 ggcaaagaag ttcccttgcc atgtatagcc gagacgtgcc tcattaatag ttgtggaact
     3061 gatattccat acatccgtaa tctgcgcatt gttattgtct acatcgccgg actgacatcc
     3121 aatgggacag gccgttactg aattgggata gatgacgggc gtgtcgctct gcgtgtcgga
     3181 catcgtcaag cgattattcg gtgtgatgtc gtaatcgagg cggccaaagt attttctata
     3241 aggggtagat tgaggaagac tcgaatagaa gttgttctgt aacaaaccaa ccgagttcag
     3301 gcttccagga acaaacttgc ctcccgggat gtgattggaa ggcgttggat aaaactgctg
     3361 aaatgctgca gcaactttat cgaacattgc ctgtggaata gcatttgatc cgtactcttc
     3421 caggaaggat ttgcgaatcg gataagggtt gccttttgca tcatgcccaa tcgtctgcgt
     3481 tgtcgggtca taaattaatg gctggcctgt gaagtctccg ctcatgactg ctgttgtggg
     3541 gaccgtatta aaagaattac ttgctgatcc atggtctaca atctggtcgt agttaaagaa
     3601 gaagaacatc ttccgcttga ggattggacc actgatggaa ccgccaaatt cgttgtaacg
     3661 aaggaaggga accgttacct tgtttccgaa gccataattt gcagcattta gagcgtcatt
     3721 ctgaaaatag tcataggctg aaccatggaa ttggtttgta ccccccttgc taatctggtt
     3781 gaaaataatt ccgccgatac cgtattgtgc tgaaaatgta gaagtattga cctgcaattc
     3841 cgagactgtt tcgaagatcg ccggattggc attttgactg tgcgacaaag tcgtggaagc
     3901 accgtcggcg aggatgttgc tataaggaag atttccattg atggccactt cctggccagg
     3961 attcgctgct ccctgacttc cgttaggagt tccggatgcc cctggcagga ggatcgtgaa
     4021 attttcccag cttacaccgg aagcgctggc tacctggggc atttgagcca tggatttagc
     4081 gtccagagta gtcacctgct cgcctgattc tgtcttgagc agcggcacat ctgagttcac
     4141 tacaaccgat tgagttgtag atccgatttt caactgagca ttgactgttg taaagccaac
     4201 ctgcagtgta atggggccac gcaccaattc atcgaagcca tcttttgaaa atgtgacttt
     4261 gtaatttcca gcaacaatcg aggaggtatc atacaagccg tcactgttag tgacaaattc
     4321 tttcgataca cccgtgtcca cattcaaaac agttactttt acatcgggaa ccacggcccc
     4381 ggatggatct gttacagtgc cacgtatgtc accagcgttg gtgctctgtg caaagccgat
     4441 cgcagttgac aggacgcctg ccactgcaat gcctcttaaa actgtcttcc acatagaagc
     4501 ctcctaaaat tttcctgttt cgagcgccag cccttcatcg ctgcatggcc tgatcagcaa
     4561 cgggccggct tcttgttttt gcactctctt tacagacaat cgtttgttcc gtacttatcg
     4621 gcaattgtcc aggaagggcc gacgatgata aaaacaattt cgtaatacgc ttgtcaagaa
     4681 aattattttt catatagctt aagacaaaca tttgtcttca tgaaaacgag gaaagcacgg
     4741 ccaaaataat gatgaaaagt acatcccgcc gcacctttct gaaactctcc gccatgaccg
     4801 ccgcgtccac ggccctgcct tcgttcgcca cggcaaacgc aggcccggtg aaagtatggc
     4861 gcacttcagc agaagagcgc tggcaaacac tggaagcgct tgcatgggaa cccttccgcg
     4921 gcacctcgcc tgtaagcatc cgcctcatcc ctgcccgtaa ataccagcag gtgcttggtt
     4981 ttggcgcggc ccttacggat gcatcctgct atctgctgga gcagcttgac gccgggaagc
     5041 gtaaaacaat tctggaagac tgctttgggc catcgggact gcggctttcg gttgcgcgga
     5101 ccacgatcgg ttcaagcgac tactcgctga gcgcctatag ttatgacgac acgcagcagc
     5161 ctgatcctgg actaaagcat ttcagtattg accatgatcg caaatacatc cttccgctat
     5221 tgcgcgaagt ccagaacgta aatcctgaga tgttctattt ctcctcgcca tggagccctc
     5281 ctggctggat gaaggcaggc ggttcgctgc ttggcggttc catgcgcagc cattactttg
     5341 acgcctatgc ccagtacttt gtgaagtttc ttcagggtta tgccgccaac ggagtgaaga
     5401 tcgaggcagt taccgttcag aacgaagtgg acactgacca ggatggccgc atgccgcagg
     5461 cgctgtgggg acaggaatat gagatgaact ttgtaaagga ccatcttggc cccgcgctca
     5521 aacaggcggg tctggataca aaaatctgga tacttgacca caactacaat ctatggggtc
     5581 gcgctgtgga cgagatcagt gatcctgatg tttatcaata cgtggatggc gtcgcgtggc
     5641 acggctacta cggtgatccc tcggccatga cccgcgtaca cgatatgttc ccagaaaaga
     5701 atgcgtattg gacagaaggc ggccccgatt acaaatcgcc agattacgca accgattgga
     5761 caaaatggtc caataccttt acgggaatcc tgcgtaactg ggcgcgatgc attgtgagct
     5821 ggaaccttgc cctggatgag aacggaaaac caaacatcgg cccatttgat tgcggcggtc
     5881 tcgtcaccgt ccattcagga acgcatgaaa tcacacgaag tggacagttc tgggcctttg
     5941 cgcattattc aaaagtcata caccgtggcg cgcatatctt tgcgtcatcg ggcgatctgc
     6001 aaaacatttc gcatgttgca gcggaaaacc cggatgggag ccgtgtgttg gtgcttacca
     6061 atacagagcg cgagcagaca gtgcaggttg tattggagga acaagcgctc tcgctgaggc
     6121 taccgcccga ttcagtaact acccttgtct ggtgagtctc cggaccttgc tcgctgcgtt
     6181 ttcgcgtacg ggcccggtag attcacgcac gatcaactgc ggctttacag caacctcagc
     6241 cgggattgct ccctgcgaac gcaacctttg caacagtagc tgtgcggaga gctttcccat
     6301 ctcgtgcaat ggctggcgga ccgtagtgag gctcggtgtt tgaaacgcag cctgtggaac
     6361 atcatcgaac ccaatcacgg agatgtcctg cggcacgcgc agttgtgcat cgtgcaaagc
     6421 acggatggcc ccgatagcgg ccatatcatt aaaacagaca atcgcagtaa agcggcgatg
     6481 atgcgccaga agtttttgca caacaggata gcccagctca ggcgaagtaa ggtcttccgt
     6541 caactcgacc gtcagttccg gtctgatggc aatgccgagt tcgcgcgctg cttttacgat
     6601 tccctgccag cgcacctctg aatcagagct gtaaggttgt ccacgcataa atgcaatgct
     6661 gcgatgtccc agatcacgca gatgacgcat cgtaagttcg gcagccgtgt tgtggtcgag
     6721 aacaacgttg gtcacgccag aaatcttacg gtgtccggcc acggccacag caggtactgc
     6781 cagcttatgg tccagatggg tgtcaatggc aataattcct tcagctccgc ggcttatcag
     6841 catccgcgga tactgctcaa tcagatcagg gcgatggcga tg
//
LOCUS       DASRBV010000166         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_724226, whole genome shotgun sequence.
ACCESSION   DASRBV010000166 DASRBV010000000
VERSION     DASRBV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724226"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..477
                     /locus_tag="VFA02_06340"
     CDS             265..477
                     /locus_tag="VFA02_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09507.1"
                     /translation="MRSEQIHRALAQGYNRFEICKLVSKGVKVTHRPGTRFEDSIGNV
                     LDQIKLSGKRHAPPSQSENEVIDPAA"
     gene            777..1895
                     /locus_tag="VFA02_06345"
     CDS             777..1895
                     /locus_tag="VFA02_06345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZU09508.1"
                     /translation="MNPFNDFNLSPQLKKKLAAANFITPTPVQAAAIPPALEGRDILA
                     TAQTGTGKTLSFLVPMIERLARNPGKGIKALVLLPTRELAMQVQTAYSKLGANHGSAL
                     IVGGLAERPQLDAIRRGASVLIATPGRLEDFLKRRLMRLDQVQMLVLDEVDRMLDMGF
                     KPAIQRIVSTLPTERQTLCYSATLDKQVKEAVHDYLRQPARIEIGSVLRPSENVKLRA
                     FEVPTAKKQMLLEHLLQSEEGSFLVFVRTKHGADRVAQKLGRSGWQATRIHGDRTQAQ
                     RTAALRSFSQGQHRVLVATDVAARGIDVEHVAHVVNYDLPKVAEDFVHRVGRTGRAQK
                     RGVASTFASPEEKGELRRMEKTLSIQMERFRVKELATA"
     gene            complement(1988..2962)
                     /gene="galE"
                     /locus_tag="VFA02_06350"
     CDS             complement(1988..2962)
                     /gene="galE"
                     /locus_tag="VFA02_06350"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZU09509.1"
                     /translation="MKILITGGAGYVGGTVAELLLRNGHEPVIYDNLCHGHRALVPSG
                     AVFIEGDIADRSRLEKVFDAHKPEGVMHFAALIEAGESMKKPELYFRNNTASTLTLLE
                     VMLSRGVNKLVFSSTAAVYGEPESTPILEDAPLRPTNAYGESKLLVEQMLTWLNRIHG
                     FRYASLRYFNVAGAIEGRGEAHEPESHLIPLVLDVALGRRKSIKVFGTDYPTPDGTCI
                     RDYIHVWDLAKAHLLAFHALADRDRLIYNIGNGQGFSIREVIESARRVTGHPIPVEEV
                     ERRPGDPAVLIASSEKIGRELGWKPEFDTLDSIVETAWKWHQERYQRT"
BASE COUNT          810 a          902 c          907 g          759 t
ORIGIN      
        1 aacgcgatgc tcgcgcagga tggaatcaag gtgcggattc aggctggttg acgtagacgc
       61 catgcgggct gaaatacctc tttctgacga aaattcttca gcatccacgt taacggttgc
      121 aagcaacgag tggcaagatg ccctcggcaa gggcgtacaa ttagcagaag acagacgaaa
      181 ctgcctgctc gcctggcggc ttttcttttc gctccctacg ggcaccccac ctcacgcagc
      241 aatcaccgga ttccggagtt catcatgcgt tcagaacaga ttcatcgcgc gcttgcgcag
      301 ggatacaacc gttttgaaat ctgcaagctc gtttcaaaag gcgtaaaagt cacccacagg
      361 cctggaacca gattcgaaga ttcgattgga aacgtgctgg accaaatcaa gctgtcaggg
      421 aagcgtcacg cccctccatc acaatcggaa aatgaagtaa ttgacccggc ggcgtaggcg
      481 catcctcgtt cgtgatatca ttaaaacacg ctcaatgatt tggatgtgag ccgcctcgcc
      541 ttttaaaata aggccgcctg ccgcaagtac ccgccaaaac cttcagcgta ttgaagcttt
      601 tttcgcgtga gctgtgtgct cgtgcagaaa gcgaaacact gtgcggcaga gccgatgcag
      661 gccatttact tcaaggactg caaagcatca tttcggaact tccctgcgta atatttttcg
      721 tgtttatgga atggatgaag ccttcctgaa cacatttctt cattcttcaa ggacaaatga
      781 atccatttaa cgatttcaat ctttcgcctc agctcaaaaa gaagctggcg gcagcaaact
      841 ttatcactcc tacacccgtc caggccgcag ccattcctcc tgcgctcgaa ggccgggata
      901 tattggccac tgcgcagaca ggcacaggaa agaccctcag ttttcttgtc cccatgatcg
      961 aacggcttgc gaggaatccg ggcaagggaa tcaaagcgct ggtgttgctg ccaacacgtg
     1021 aactggccat gcaggtgcag accgcataca gcaaacttgg agcaaaccac gggtcggcct
     1081 taattgtcgg cggtctggcg gaaaggccac aactcgatgc catccggcgt ggcgcttctg
     1141 tgctcattgc aacgccgggg cgtcttgagg attttctgaa acggcgtctg atgagactcg
     1201 atcaggtgca aatgcttgtt ctggacgaag tggaccgcat gctcgatatg gggtttaagc
     1261 cggccatcca gcggattgtg tccacgcttc ccacggaacg gcagacgctg tgctattcgg
     1321 caacactcga caagcaggta aaggaggccg tacatgacta tctgcggcaa cctgcccgga
     1381 tcgagatcgg ctctgtgctc aggccatctg agaacgtgaa gctgcgtgcc tttgaagtcc
     1441 ccacggcaaa aaagcagatg ctgctcgagc acctgttgca gtctgaagaa ggcagttttc
     1501 tcgtgtttgt caggacgaaa cacggcgcgg accgggtggc ccagaagctc ggacgttccg
     1561 gctggcaggc gacgcgcatc cacggtgacc gcacgcaggc gcagcgcaca gccgctttgc
     1621 ggagcttttc gcagggacaa caccgcgtgc tggtggcaac cgatgtggcc gcgcgcggca
     1681 ttgatgtgga gcacgtagcg catgttgtga actacgacct gcccaaagtg gccgaagatt
     1741 tcgtgcatcg cgttggccgt acaggccgcg cgcagaaacg cggcgtggcc tctacgtttg
     1801 cttctccgga agagaaaggc gaactgcgcc gcatggaaaa gacgctcagc atccagatgg
     1861 agcgcttccg cgtgaaagag ctggcgacgg catagctgta tcctgggcct gcttttcaat
     1921 ttcagaccca ggacatgagg gcctgtccac acaagccgga cagtagcttt gtgtgaacag
     1981 gcccttgtta tgttcgctga tatcgctcct ggtgccattt ccacgccgtt tcgacgatgc
     2041 tgtccagcgt gtcgaactca ggtttccagc caagttcgcg gccgattttc tccgagctgg
     2101 cgattagcac agcgggatca ccggggcgcc gctcaacctc ttccactgga attgggtgtc
     2161 cggtgacgcg acgggccgac tcaatcactt cccgaatcga aaacccctgc ccatttccga
     2221 tgttatagat cagccggtcg cgatcagcca gggcgtgaaa ggccagcaag tgtgcctttg
     2281 ccaggtccca gacatggatg tagtcgcgga tacaggtgcc atctggagtg gggtagtcag
     2341 ttccgaagac cttgatgctc ttgcgccgtc ccagggccac atccagcacc agcgggatca
     2401 gatgcgattc cggctcgtgg gcctcgcccc ggccctcaat ggcacccgca acgttaaagt
     2461 agcgcaggct ggcgtagcgg aagccgtgaa tgcggttcag ccatgtcagc atctgctcta
     2521 ccaacagttt tgactcgccg taagcattcg tcgggcgcag cggagcatct tccagaatgg
     2581 gtgtggattc cggttcacca tacacagcag ccgtcgaaga gaagaccagc ttgttcacgc
     2641 ctctggacag catgacttcc agcagtgtca gagtagatgc cgtattgttg cgaaagtata
     2701 gctccggctt cttcatgctt tcgcctgcct cgatcagggc ggcaaaatgc atcacgcctt
     2761 caggtttgtg ggcgtcgaaa actttttcca gccgggagcg gtcggcaata tctccctcaa
     2821 taaaaactgc cccggaggga acaagagcgc ggtgtccatg acataaattg tcataaatta
     2881 caggctcatg gccatttctg agcaacaatt ccgcaaccgt tccgccaaca taacccgcgc
     2941 cgccggtgat cagaattttc aattgtatct ccttgtaaat atttagatta gaggaactac
     3001 atcatcgaat tttgcttttg agagcagctt aaagattctt aaagtgcatc caaaacggac
     3061 tgaaagtaaa gattacacgg atgcaggtac gaattgcgga gtaaggacta ccaggaaatt
     3121 ctacccaatt acttgaaagc acaagggatt gacttttcaa tgaaaactca ggatgagcgg
     3181 ttcccgtgaa tatgagatag taatcatagc tgtaaaatat gacagcgccg gtgttgtcac
     3241 actttggcaa ctatcttgct ataacaaagt gaatgagaaa ttagaaggca agatgttccg
     3301 ccttccgggc cctggaattt gaaataagac gtttgttttt tcgtctattt ttttgaagaa
     3361 ggttatttcg gtcaactg
//
LOCUS       DASRBV010000167         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_314004, whole genome shotgun sequence.
ACCESSION   DASRBV010000167 DASRBV010000000
VERSION     DASRBV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314004"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFA02_06355"
     CDS             <1..283
                     /locus_tag="VFA02_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09510.1"
                     /translation="GKESVIPALANVRSYRYWALSPDGIYYVSGNNKNTRYLVNFYDF
                     TTHHITPIFEIERPLYSGTPSLSVSPDGHYLAYAQVDSFHANLVLAENW"
     gene            415..1299
                     /locus_tag="VFA02_06360"
     CDS             415..1299
                     /locus_tag="VFA02_06360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZU09511.1"
                     /translation="MYNQTVRLAASMTQQADKVEEVQVVAEAIPAAWLKTELARHPQR
                     PYPMDFIKSIFTDFSEIHGDRAFGDDPAMCCGMARFDGEEVMVIGNLKGRTVKERVQR
                     KFGSPEPEGYRKALRAMRMAEKFGRPVFTFIDLAGAYPGIGAEERGQAEAIARNLLEM
                     ARLRVPIVATITGEGGSGGALALAVADRVLMLENAIYSVISPEGCASIMWRDASKRAQ
                     AAEALKLTAEDVKSLGCVDDIVPEPQGGAHADSEAAARLLKEKLKWHFGEVKSLPEEE
                     MLQRRYEKFRNIAQFYTA"
     gene            1330..2385
                     /locus_tag="VFA02_06365"
     CDS             1330..2385
                     /locus_tag="VFA02_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZU09512.1"
                     /translation="MRSAGRFRSFSWFAIAAIYYIFAQQVSLRAANGLSSGDWFPLVN
                     SLILLFLLLVGYAGMGYAAQHQQQPLKIMGLVRREGWKREFAVGAAIGWVGVVACVLP
                     VAVFGGLVITFWKGGHQYFLLLVHLTTLAALSLAQEIAFRGYPFQRLMDSIGPTLATL
                     FMPVLYTAIHMMNTDASAASVLVTILLGWLLSIAYLRTRALWVPWGIHFAWNASMALL
                     FGLPVSGMTTFNPVISSNTVGPPWLTGGDYGPEGSAIAVIVALLLMIVLVKATRDYAH
                     RYAQPVIVPAGMPVDIDTMARRQHEAAMGAVPNPSEPQLVQISPAMPSGNGTTSSSSS
                     ESGPEKPRFVDEEGEKV"
     gene            2506..>3554
                     /locus_tag="VFA02_06370"
     CDS             2506..>3554
                     /locus_tag="VFA02_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09513.1"
                     /translation="MQGFAARRMLRVLAGGTLFLLTLAGSSPAQVFTVSPEKIEGHYL
                     DFHPTNVPLPAQPITERGRQELIRFLTAEQGFAMRPLPRATLTLHANGDLTPSGSDYV
                     NVINEHGVSAKPGDRCVITDVKIKEDSIVLLLNGGPDPKHKWLRHVSIGMDPYDTTPV
                     VQDSGPEPVGSRITLVFPHRVPEVTGQQVEALLSPVLGFGVKSPVEAYTDTLPPWLKK
                     AILDHHVLVGMSTQMVLYTLGQPQQKIRERDGQMPFEVWVYGEAPNPIQFVRINGNRV
                     IRVEIGEVGKPLVVRTQNEMGDYWNTAPAPNVREVKMGDQDPTAIAQQSAPKAPPTLR
                     KPGETLPTDNDKNHPT"
BASE COUNT          788 a          944 c         1025 g          797 t
ORIGIN      
        1 aggcaaagag tcggtcattc ctgcgctggc aaacgtcaga agttaccgct actgggcgct
       61 ctcgcctgat ggtatttact acgtttcagg gaacaacaaa aacacacgct acctcgtaaa
      121 tttttacgat ttcacaacgc accacatcac tcccattttt gaaatcgagc gccctcttta
      181 cagcggcact ccaagtctct ctgtttcgcc cgatgggcat taccttgcct atgctcaggt
      241 agattcgttc catgccaacc tagtgcttgc agaaaactgg tagattcaga atgcctcggc
      301 ccgggtgctg cccacttcga tggactacca gatgaagccg cctgtgaatt gggcctgata
      361 aagtaaccat gctctgggtc aaaaatcgtg gcgccatggt ttacatcctc tgccatgtac
      421 aatcagacag taagacttgc agcgtctatg acacagcagg cagacaaagt agaagaagtg
      481 caggtggtgg ccgaggccat tcctgcggcg tggttgaaaa cggagctggc gcgtcatccc
      541 caacgcccgt atccaatgga ctttatcaaa agcatcttca ccgacttcag cgagatccat
      601 ggcgaccgtg cctttggcga cgatccggcg atgtgctgcg gaatggcgcg ctttgatggg
      661 gaagaagtca tggtcatcgg caatctgaaa gggcgcacgg tgaaggagcg cgttcagcgt
      721 aaattcggct cgcccgagcc ggaagggtac cgcaaggcgc ttcgcgccat gagaatggcg
      781 gaaaaatttg gccgtccggt cttcactttt attgatctgg ccggggcata ccccggcatt
      841 ggtgcagaag aacgcggaca ggccgaggcg attgcgcgca atcttctgga gatggcccgg
      901 ctgcgtgttc cgattgtggc cacaattacg ggtgagggcg gatcaggcgg tgctctggca
      961 ttggcagtag cagaccgcgt tctgatgctg gaaaacgcaa tctattctgt tatttcgcca
     1021 gaaggctgcg cgtctattat gtggcgcgac gcatccaaaa gggcgcaggc tgccgaggcc
     1081 ctgaagctga cagccgaaga tgtgaaatcg cttggctgcg tagacgatat tgtccccgag
     1141 cctcaaggtg gagcgcacgc tgattctgaa gcagcagccc gtctgctgaa agaaaagctg
     1201 aagtggcatt ttggtgaggt caaatcgctg cccgaagaag agatgcttca gcgccgctat
     1261 gagaagttcc gcaatatcgc gcagttttat accgcatgag atagtcattc ataggccaaa
     1321 aggtgcgatt tgaggtctgc cggacgtttc cgttccttca gttggttcgc gattgcggcc
     1381 atctactata tctttgctca gcaggtgtct ctccgcgctg ctaacggact cagttccggg
     1441 gactggtttc cgctggtaaa cagcctcatt ctgcttttcc tgctccttgt tggatatgca
     1501 ggcatggggt atgcggcaca gcaccagcag cagccgctca aaatcatggg ccttgtccgg
     1561 cgtgagggat ggaagcgtga gttcgccgtt ggtgcggcga ttggatgggt gggggtggtc
     1621 gcttgtgttc tgcccgtcgc tgtattcggc ggtctggtga ttaccttctg gaagggtgga
     1681 caccagtatt ttctcctgct ggtccatctg acgacgctcg cagccctatc tcttgcgcag
     1741 gagattgctt ttcgcggata tccgtttcag cggttgatgg actcgattgg gccaaccctg
     1801 gcgacgttgt tcatgcctgt gctctatacg gcgattcata tgatgaacac ggatgcgagc
     1861 gcggccagcg tacttgtcac cattctgctg ggatggctgc tgtccattgc gtatctgcgc
     1921 acgcgggcgc tctgggtgcc gtggggcatc cattttgcgt ggaacgcgag catggcgctt
     1981 ctttttggcc ttcctgttag cggaatgact acattcaatc cagtgatttc ctcgaatact
     2041 gttgggccgc cgtggctcac aggtggagac tatggaccgg aaggaagtgc aattgcagtc
     2101 atcgtagcgc ttttgctgat gattgtgctg gtaaaggcca cacgcgacta tgcgcacaga
     2161 tacgcgcagc cggtcatcgt tcctgccggg atgccagtag acatagacac gatggcccgg
     2221 cggcagcacg aagcggccat gggcgcggtt ccaaatccat cggaaccaca actggtacag
     2281 ataagccctg ccatgccttc cgggaatggc acaacgtctt catcttcatc cgaatccggg
     2341 cctgaaaaac cgagattcgt tgatgaagaa ggagagaagg tttgaaacag cacgccaagc
     2401 cgcttttttc tactcaaaac ggaatttgca cggactgccg tgtaaggact tgcaatcggt
     2461 gatgaatcgc gccatgcttg tagaccaaga gcaaagaggc ccgtcatgca gggattcgca
     2521 gcccggcgta tgttgcgagt tttggcaggg gggacactat ttctgcttac tttagcaggg
     2581 agctccccgg cccaggtctt caccgtcagc ccggaaaaga ttgaaggcca ttatctggac
     2641 tttcacccca cgaatgtccc tcttccagcg caacccatta cggagcgagg ccgtcaggaa
     2701 ctcattcgct ttctgaccgc ggagcagggc tttgccatgc gtcctctgcc gcgtgccacg
     2761 ctaacactcc atgccaatgg agaccttacg ccgagcggct cagactatgt gaatgtgatc
     2821 aatgaacatg gcgtttctgc caaaccgggt gaccgttgcg tcatcacgga cgtgaaaatc
     2881 aaagaggaca gcatcgtcct gctcttaaat ggcggccctg atccaaagca caagtggctg
     2941 cgccatgttt cgattggcat ggacccctat gacactacac cagtggtgca ggacagcggt
     3001 ccagagccgg tgggctcgcg tattacattg gtctttccgc atcgagtacc ggaagttacc
     3061 ggccagcagg tggaggcgct actgtctccg gtgctggggt ttggtgtgaa atcacccgta
     3121 gaagcataca cagatacgct gcctccgtgg ctaaagaagg ccattctgga ccatcatgtg
     3181 ctggtgggca tgagcacaca gatggtgctc tacacgcttg ggcagccgca gcaaaaaatc
     3241 cgcgagcgcg atggacagat gccgttcgag gtatgggtgt atggagaggc ccccaatcct
     3301 atccagttcg tgcgcatcaa tggaaaccgc gtgattcgtg tggaaattgg agaagtaggc
     3361 aagcctttgg ttgttcgcac acagaatgaa atgggcgatt actggaacac cgcgcccgca
     3421 cccaatgtgc gcgaggtgaa gatgggagac caggacccca cagcgattgc gcaacagtct
     3481 gcgccaaagg caccgccaac gctgcggaaa ccgggtgaaa cacttccgac agacaatgac
     3541 aaaaaccatc ccac
//
LOCUS       DASRBV010000168         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_314154, whole genome shotgun sequence.
ACCESSION   DASRBV010000168 DASRBV010000000
VERSION     DASRBV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314154"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFA02_06375"
     CDS             complement(<1..551)
                     /locus_tag="VFA02_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU09514.1"
                     /translation="MANLLAIKPLSTLKQEAAEEGEHTLRRSLGPINLITLGVGAVIG
                     AGIFVLTGQAAALHAGPAIALSFILAGITCAFAGLCYAEFASLIPIAGSAYTYGYATL
                     GELVAWIIGWCLCLEYAFGAATVASGWAGYFLSLIEQIGITPSASLARFTTTYGNVIY
                     LYDHRWMPQASLPPGVDFSSLPHV"
     gene            complement(577..2076)
                     /locus_tag="VFA02_06380"
     CDS             complement(577..2076)
                     /locus_tag="VFA02_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU09515.1"
                     /translation="MAQRLGLFSSTAIVVGSMIGSGIFIVPAEIGRGVGSPALLIAAW
                     LVTAVMTIIGALSYGELAAMMPSAGGQYVYLREALGPMWGFLFGWTLFLVIQTGTIAA
                     VVVAFGKFLGVFFPAVSSSHWLLHLGHVPALRIGPMVLGNMEIGLNTANLTGIVIVLL
                     LTGINILGVRLGALVQNIFTTAKTAALLGLVVVGFWFGRNPEAIAANFGHNFWQNASW
                     HTLHPVQVGVGGPIAMVGLLAVLAVVQTGSLFSADAWNNVTYTAGEIRNPKRNLPLSL
                     VLGTVIVIALYVTANFVYLCALPLHGDPNGATAFARGIQYASEDRVATVALEQIFQSS
                     GAFLMAGAILISTFGCANGLILAGARVYYAMSRDGLFFQSTSTLHPRYKTPVWALVIQ
                     ALWTCFLCLTGSYSQLLDYIICTELIFYILTICCLYVLRKRKPDAERPYRAVGYPVLP
                     AVYILMAAWICVVLLRYKPQYTWPGLFIVVLGVPVYLIWSRRRQTAIQD"
     gene            2333..3040
                     /locus_tag="VFA02_06385"
     CDS             2333..3040
                     /locus_tag="VFA02_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU09516.1"
                     /translation="MFEDSLLESTGRIRTHSRYFAVGSFALQALLLTALVLYPLAHPA
                     LLPKQSLTMLLTAPPLPSAPANLPERTAASPARPALLVNPLAMPSRIPQHAVMVTEAL
                     PSGVDSRFAQSNGSIPGGIFDLIGSVPGQPVVKPAPVKGPIRVSSGVAAGQLLTSIQP
                     VYPAIAKEAHISGTVVVQAMIAKDGSIQHVQVISGPPLLRQAALDAIARARYRPFLLN
                     GEPVEIETTINVVFTMN"
     gene            3135..4580
                     /locus_tag="VFA02_06390"
     CDS             3135..4580
                     /locus_tag="VFA02_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZU09517.1"
                     /translation="MGPYFGSVWMARLLFQRGMAATYLIAFIVVFRQFKPLLGERGLL
                     PVPAYLKNTPFWNKPGLFCWHYSDGLLDVVAWAGIIVSAFALCGFTEAGPLWLSVGAW
                     LLLWFLYLSIVNAGQTFYGFGWESMLLEAGFFTAFLGPSRMAPSVLPILILRWVLFRT
                     EVGAGLIKLRHDPCWRQLTCLFYHYETQPLPNPLSWYFHRLPAPFHKFSVLFSHFVQI
                     IAPFGLFAPQPIAAIAGGLIIFHQIWLIVSGNYSWLNWLTVVLGFTAFRDPASFTTPQ
                     LALRPVAYDLLLYFITGATVWLSIEPVKNLFSKHQVMNRSYNSFHLVNTYGAFGQVGR
                     KRYEIVIEGTDECVITPYTQWREYGFKAKPGDPKRMPPQVAPYHLRLDWLVWFLPFTV
                     AITSRGIQVRRYDVWFLRFIQKLLKGDPALLKLIGKDPFPNQPPAFIRALFYEYRYTD
                     RQQKRETGAWWIRQQLGIYLPPVSLNDLKRI"
     gene            complement(4592..>4846)
                     /gene="lepB"
                     /locus_tag="VFA02_06395"
     CDS             complement(4592..>4846)
                     /gene="lepB"
                     /locus_tag="VFA02_06395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZU09518.1"
                     /translation="DLVVPPGHYFAMGDNRPVSLDSRYWGFVPRENIVGRPLFVYWSF
                     VTPENQIDKTSMADKVAFIGHIILHFFDQTRWSRTLHLVK"
BASE COUNT         1135 a         1459 c         1191 g         1061 t
ORIGIN      
        1 acatgcggca gcgacgaaaa atccactccc ggaggcaaag aagcctgtgg catccagcga
       61 tgatcataca gataaatcac attgccgtat gtggtcgtaa accgcgccag cgaggccgac
      121 ggtgtaatgc cgatctgctc aatcaggctc aggaaatagc ccgcccatcc cgaggccaca
      181 gttgccgcgc caaaggcgta ctcaaggcac aggcaccagc cgatgatcca tgcaaccagc
      241 tcgcccagag tggcatatcc ataggtgtag gctgacccgg caatcggaat cagcgaggcg
      301 aactccgcgt agcagagtcc ggcaaaggcg caggtaattc ctgccaggat aaagctcagc
      361 gcaatcgccg gccctgcatg gagtgcggca gcctgtccgg taagaacaaa aatgccggcg
      421 ccaatcacag cacccacgcc gagcgtaatc agattgatcg gcccgagtga tcgccggagt
      481 gtatgctctc cctcttcagc agcttcctgc ttgagtgtgg agagcggttt aattgctaac
      541 aaattcgcca tgtacgagcg ctttcccctt ccagcttcaa tcttgtattg ctgtctgcct
      601 gcggcgcgac catatcagat aaacgggcac gcccagaaca acgataaaca aacccggcca
      661 cgtgtactgc ggcttgtagc gcagcagcac cacacagatc cacgctgcca taagaatata
      721 gaccgctggc agaactggat atcctaccgc acgataagga cgttcggcat ctggcttccg
      781 ctttcgcagc acataaaggc agcaaatcgt cagaatatag aagatcagct ccgtgcagat
      841 gatgtaatcc agcaactgac tataggatcc tgtaaggcat aggaaacacg tccagagtgc
      901 ctgaatcacc agcgcccaga caggcgtttt atatcgcgga tgcaacgtac tcgtggactg
      961 aaaaaacagc ccgtcacggc tcatggcgta atagacgcgt gcgcctgcca gaatcagtcc
     1021 gttggcacat ccaaaggtcg aaatcaggat cgcgcccgcc atcagaaatg cgccactgct
     1081 ttgaaatatc tgctccaatg ccactgtggc cacgcggtct tctgaagcat attgaatgcc
     1141 gcgcgcaaat gccgttgcgc cattcggatc accatgcaac ggcagcgcac acagatagac
     1201 aaagttcgcc gtcacataaa gcgcaatgac gatgaccgtc cccagcacca gcgaaagcgg
     1261 caggttccgc ttcgggtttc gaatttcgcc cgccgtataa gtcacgttgt tccaggcatc
     1321 ggcggaaaac aatgaccccg tctgtacgac agccagcacc gccagcagcc caaccattgc
     1381 gattggcccg ccaacaccta cctgcaccgg atgcagcgta tgccacgacg cattttgcca
     1441 gaaattgtgc ccaaagttcg ccgcaatcgc ttccggattt cgtccaaacc agaaccccac
     1501 aaccaccagc cccagcaatg cagccgtttt tgctgttgtg aaaatgttct ggaccagtgc
     1561 ccccagtcgc acgccaagaa tgttgatccc ggtcagcagc agcacaatca caatgccggt
     1621 aaggttggcc gtattcaggc caatttccat attgcccagc accatcggcc cgatgcgcag
     1681 cgcgggaaca tgccccagat gcaacagcca gtgcgaagaa gaaactgcag gaaagaaaac
     1741 gccgagaaac ttgccaaatg ctacaacgac ggcagcaatc gtcccggtct ggatgacgag
     1801 aaaaagcgtc caaccaaaaa gaaagcccca catagggcct aatgcttctc tcagatatac
     1861 atattgtccg cccgctgagg gcatcatcgc cgccagctcg ccatagctga gcgcgccgat
     1921 gatcgtcatc accgccgtga ccaaccaggc cgcaatcagc aacgccggag agccgacacc
     1981 gcggccaatc tccgctggaa caatgaatat ccctgagcca atcatggaac cgaccacaat
     2041 ggccgtggaa ctgaaaagcc caagccgctg ggccaggtgc ggagtggcag attgcgtctg
     2101 tgctggaaca gtccgggtac tagtactcac ttcttgatgt tctacagaaa aaatgcagac
     2161 aaagccatta ctttcttgca ggaaagcctt tgcatttgat ggtatcagca aaatttattc
     2221 agccagattc gtagaaccgc acgcgcctcg gattcaggct caaacctctc ttccgcggtg
     2281 tctattttcc cgggtgtctc gctgacttaa atcccatctc tcaggagaca ccatgtttga
     2341 agacagcctg ttagagtcca ccggacgcat tcgtacccac agccgttact tcgcagttgg
     2401 atcatttgca ttgcaggccc tgttgctcac cgcccttgtg ctctacccgc ttgcacatcc
     2461 agccttgctg ccaaagcagt cgcttacgat gctgctgacg gccccgcctt tgccttcagc
     2521 accagcaaac cttccagagc gtacagcggc ttcaccggcc cgccctgcct tgcttgtcaa
     2581 cccgctcgcc atgccatcgc gaatcccgca acacgccgtc atggtcactg aagccctacc
     2641 gtccggggtc gattcccgct tcgcccaaag taacggatct ataccgggcg gaatatttga
     2701 tctgatcggc tcagtacctg gccaacccgt cgtgaagccc gctccggtga aaggccccat
     2761 tcgtgtctcc tccggagtag cagccggaca acttctcacc tcaatccagc ccgtctatcc
     2821 tgccatcgca aaggaggcgc acatctccgg aacagtcgtc gtccaggcca tgattgcaaa
     2881 agacgggagc atccagcacg tgcaagtcat cagcggaccg ccactgctgc gtcaggcggc
     2941 actggatgcc attgccaggg cccgctatag gccgtttctc ttaaatggag agccggtcga
     3001 aatagaaacc actatcaatg tggtctttac aatgaattga ttcaacgtga atgcgcccat
     3061 gggccggaaa gaaaacgatt cgctccggcc cacgcgcatc gtgacatagc tatctcaatc
     3121 atggtgaggc tctcatgggc ccctacttcg gttccgtatg gatggcgcgc ctgctttttc
     3181 agcgaggtat ggcggcgacc tatctcatcg ccttcatcgt cgtgtttcgc caattcaagc
     3241 ctcttcttgg cgagcgcggc ctgctgcccg tgcctgcgta cctgaagaac actcctttct
     3301 ggaacaaacc aggactcttt tgctggcatt attccgatgg cttgctggac gtagtagcgt
     3361 gggcaggcat catagtttcc gccttcgcgc tgtgtggctt tacagaagcg ggccctctct
     3421 ggctttccgt cggcgcatgg ctgctcctgt ggttcctgta tctctccatc gtcaatgccg
     3481 gacagacatt ctatggcttt ggatgggaat ccatgttgct ggaggcaggc ttctttaccg
     3541 cattcttggg gccttcgcgc atggccccat ctgtcctgcc aatactcatc ctccgctggg
     3601 tgctcttccg tactgaggtt ggtgccggcc tcatcaaatt gcggcatgat ccctgctggc
     3661 ggcaacttac ttgcctcttc tatcactacg aaacgcaacc tctgcccaat ccattaagct
     3721 ggtacttcca ccggctgcct gcgccgttcc acaaattctc agttctcttc agccatttcg
     3781 tgcagatcat tgcgcctttc ggattgtttg ctccgcaacc gattgctgcc atcgccggag
     3841 gcctgattat ctttcaccag atctggctta ttgtaagtgg taattattcc tggctgaact
     3901 ggctgaccgt cgttcttgga ttcactgcct ttcgtgaccc ggcatctttt acaactccgc
     3961 agcttgccct gcgtccggtt gcatatgacc tgcttttgta tttcatcact ggggccacag
     4021 tatggctgag tattgaaccg gtcaagaacc tgttttcaaa acatcaagtc atgaaccgca
     4081 gctacaactc ttttcatctt gtgaatacct atggcgcatt cggacaggtg ggcagaaaac
     4141 gatatgaaat tgtcattgaa ggtaccgacg agtgcgtcat tactccatat acccagtggc
     4201 gggagtatgg cttcaaagcc aaacccggtg accccaaaag aatgccccca caggttgcgc
     4261 catatcacct gcggcttgat tggctcgtct ggtttcttcc cttcaccgtt gcaatcacca
     4321 gccgcggaat tcaggtacgg agatacgatg tatggttcct gcggttcatt cagaagctgc
     4381 tcaaaggcga cccggcactg ctcaagctga taggaaagga cccattcccc aaccagcctc
     4441 ctgcttttat tcgggccctt ttttatgaat atcgctatac cgaccgccag caaaaacgcg
     4501 aaaccggagc atggtggata cgtcagcaac tcggcatcta tctgcctccg gtttcactta
     4561 acgatttgaa acgtatctga gctcacggta ttcacttcac cagatgcagt gtcctgctcc
     4621 agcgggtctg gtcaaagaag tgcaaaataa tatgcccaat aaacgcgact ttgtctgcca
     4681 tggatgtctt atcaatctgg ttttccggcg ttacaaaaga ccagtagacg aatagcgggc
     4741 gtcccacaat gttctcgcga ggaacaaacc cccaatagcg gctgtccagg ctgacggggc
     4801 ggttgtctcc catggcaaag tagtgtccgg gagggaccac gagatc
//
LOCUS       DASRBV010000169         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_364535, whole genome shotgun sequence.
ACCESSION   DASRBV010000169 DASRBV010000000
VERSION     DASRBV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364535"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1720
                     /gene="accC"
                     /locus_tag="VFA02_06400"
     CDS             209..1720
                     /gene="accC"
                     /locus_tag="VFA02_06400"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZU09519.1"
                     /translation="MKFRKVLIANRGEIALRIIRACRELGIGTVAVYSDADRAALHVL
                     EADEAYRLGPAPAAESYLRGNLILDIAHKTKADAIHPGYGFLSENANFAEACEKARVK
                     FIGPPSSAMRALGSKTSARQVADSVKMPRTPGSVKGLASLEEAHAVAAEIGYPVMLKA
                     AAGGGGKGMRAVFFANELASAFAAAASEAERAFGSGEVYLEKLVERPRHIEIQVLADE
                     HGNCVSLGERECSVQRRHQKVIEEAPSAIVDEDLRSRMGEAAARFAKSAGYTNAGTVE
                     FLVDEAKNFYFLEMNTRLQVEHPVTELVTGLDLVHLQIHIAQGGKLPFKQEDIRLRGH
                     AIECRIYAEDPENGFFPSPGKITRLLQPGGPGIREDSGIYEGWVVPMEYDPMLSKLVG
                     YAQNRNVAIRRLLRALDEYVIGGIRSNLNLFRTILRDQDFQSARIDTGYLDRLLAAKT
                     ITQTSAPEREEIVAIAAALFESMKPVNGDPSNSPVNGRTSNWKQAARREALRA"
     gene            1717..2223
                     /locus_tag="VFA02_06405"
     CDS             1717..2223
                     /locus_tag="VFA02_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZU09520.1"
                     /translation="MMVQLEVNGELRRVELVPDELPGRLRAVLDGEKETEIQAHLLRP
                     GVLSLHIAGKAYRCVLEEGPEGTVIHFAGTRIPYRIDDPRSLKSRRSRAGRHEGPKAV
                     TAPMPGRVIRVLVNKGDEVQAHQGIVVIEAMKMQNELKSPKAGRVIELRVQPGGTVAA
                     GEVLAVVD"
     gene            2256..3014
                     /locus_tag="VFA02_06410"
     CDS             2256..3014
                     /locus_tag="VFA02_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU09521.1"
                     /translation="MAPVEMSVLYNQRVQPIIIAENLSKNYHVGKIDVLALRNATFSV
                     ERGEFVSIVGPSGSGKSTLFYILGGLTQPTSGRVIIDGTDFSALSDSERTNMRKSKIG
                     FVFQRFNLLPTLSALQNIEIAYEISGRKEPLDRNYLDHLTDLLGIRGRLTHRPAELSG
                     GEQQRVAMARALITHPAIILADEPTGNLDSKNSEAVLDMLMRSNRELNQTILMITHNP
                     EAAVIADRILHMKDGEVVSIEAGKGQGRVSYVGL"
     gene            complement(3022..>5040)
                     /locus_tag="VFA02_06415"
     CDS             complement(3022..>5040)
                     /locus_tag="VFA02_06415"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZU09522.1"
                     /translation="GFVLDHFDRTAIDHHLHTVGDRLMTAFGDQAPYSVFSDSLEVYG
                     SDWTPDFLDQFKRRRGYDLTPYLPELFFGNDETAKSVRHDWGQTLCELIDENYLQPIT
                     AWAHQHHTLFRSQTYGAPAVTLSSNNLVDLPEGEGPQWRSFSYTRWATSASHLYGRPV
                     TSAEAWTWLHSPAFRAVPLDIKAEADLFFLEGVNQFVGHGWPYSPPYAGDPGYSFYAA
                     AVFNDHNPWWPVMPEITQYLQRVSFLLRAGKPANDVAVYLPTDDAQANFTPGHVDLAD
                     DIRKYVTPELTASILDAGYNLDYIDDAAIQKLGIQYPVLILPHVDRIPLTVYRVIAEY
                     AQKGGKVIAVGRLPEHAPGLREFNADSPQIAAISQKLFPSAIPESETGTQLRKLLLPD
                     MQLATPAPEIGFIHRKLPNADIYFVANTSNHRVTTTARFRTQWKFAEEWDALYGKRYR
                     ANTRDTAISLAPYESRAFIFCDDDEKLPTEWHGQPDTAKQLDLSQNWQVSFDNIHRTE
                     TMQHLASWTKDEDTRYYSGTATYTRDFDLPAKMLKPGQHIFLDFGEGNPLPPPSDTKK
                     PGTHALLEGPVREIAIIRINGQKAGSIWAPPYRLDVTSFLHTGKNHVEIEVANTGINT
                     LAGQARPDYRLLNLRYGQKFVPQDMDHLEPLPSGILGEIVLTPEVPPK"
BASE COUNT         1140 a         1287 c         1489 g         1124 t
ORIGIN      
        1 tctttcagaa cttccacgcc cgcgtcggtc gcaaggtggt ggatgctgac caccattgcg
       61 tgcttcatcc ggacaggagt ttcatgctgc gtcgtctgga cgggctgggc ctgcaacgaa
      121 aaagaacaaa gatagaaaac cgcagaaagg acaaacatgc gttggctcat ggttggtgcc
      181 agtgtaacgc gataaaatcc attctggaat gaagttccgt aaggtcctaa tcgccaaccg
      241 tggcgagatc gctctgcgca tcattcgagc atgccgggag ttgggaatcg gcaccgtcgc
      301 tgtttactct gacgcagacc gtgccgccct acacgtgctt gaagccgatg aagcgtaccg
      361 tctcggtcct gctccggcag cggaatcgta tctgcgcggt aatctgattc tcgacatcgc
      421 acataaaaca aaagccgacg ccattcatcc cggttacggt tttctttcgg aaaatgcgaa
      481 ttttgccgaa gcgtgtgaaa aggccagagt gaaattcata gggccgccgt cctctgccat
      541 gcgcgcgctt ggctctaaga cgagcgcccg tcaggtggct gattccgtaa agatgccgcg
      601 tacgcccggc tcagtgaagg gccttgcttc gctcgaagaa gcacatgccg ttgccgcaga
      661 aattggctat cccgtcatgt tgaaggccgc ggcaggcgga ggcggcaagg gaatgcgcgc
      721 cgtcttcttt gcaaatgaac tggcctctgc ctttgcagca gcagcaagtg aagccgaacg
      781 cgcctttggc tctggcgaag tttatcttga aaagttagtt gaacgcccgc gccacatcga
      841 gattcaggtg cttgctgatg aacacggcaa ctgtgtctca cttggcgagc gcgaatgttc
      901 tgtgcagcgt cgccaccaga aggtgattga agaagcacct tcggccattg tggatgaaga
      961 cctgcgtagc aggatggggg aagccgctgc gcggtttgca aaatccgccg gatacaccaa
     1021 cgcaggcacg gtcgaatttc ttgtagatga agcaaaaaac ttctacttcc tcgaaatgaa
     1081 cacgcgtttg caagtggagc accctgtcac ggagcttgtc acgggtctcg atcttgttca
     1141 tttgcagatt cacatcgctc agggcggcaa attgccgttt aagcaggaag acatccgcct
     1201 gcgaggacat gcgattgagt gccgcatcta tgcggaagac ccggagaacg gcttctttcc
     1261 ttcaccagga aaaatcacgc gtctactgca gcccggcgga ccgggaatcc gcgaagactc
     1321 gggcatatat gaggggtggg ttgtgccgat ggaatatgat cccatgctct caaaactggt
     1381 cgggtacgcc caaaacagga atgtggccat acgcaggctg ctgcgtgcgc tggatgagta
     1441 tgtgatcggc ggtatccggt ccaatctcaa tctctttcgt acaatcctgc gcgaccagga
     1501 ttttcagtca gcgcgcattg atacgggtta tctggatcgc ctgctggcag cgaagaccat
     1561 tacgcaaacc tctgctccag agagagaaga aattgttgcg atcgccgctg ctttgtttga
     1621 aagtatgaag ccagtcaatg gcgacccatc caattcaccc gtaaatggca gaacaagcaa
     1681 ctggaagcag gccgcccgcc gggaggcgct cagagcatga tggtgcaact tgaggtaaac
     1741 ggcgagctac gccgtgttga gcttgttccc gatgaattgc ctgggcggct ccgtgccgtt
     1801 ttggatggcg aaaaggaaac ggagattcag gcacatttgc tgcgccccgg agtgctttcg
     1861 ctccatattg ctggaaaggc gtatcgctgc gtgcttgaag aaggtccgga aggcacggtg
     1921 attcattttg ccggaacccg cattccttat cgaatagacg atccgcgatc gctgaagtcg
     1981 cggcggagca gggctgggag acatgaaggg ccaaaggcag ttactgctcc tatgccggga
     2041 cgcgtcatac gcgtgcttgt caataagggc gatgaggtac aggcgcatca gggcatcgta
     2101 gtaattgaag ccatgaagat gcagaacgag ctgaaatccc ccaaagctgg acgagtcatc
     2161 gagctgcgcg tccagccggg gggaactgtg gctgcggggg aagtcctggc tgtcgtggat
     2221 tgagcatgag agatgacggt tgccaggggc gaagaatggc acccgttgaa atgtcagtcc
     2281 tttacaatca aagagtgcag cccatcatta tcgctgagaa cctttcaaaa aattatcacg
     2341 ttggcaagat tgatgttctg gcccttcgca atgcaacgtt cagtgtggag cgaggcgagt
     2401 ttgtcagcat tgtaggccct tcgggaagcg gtaagtctac gctcttctac atacttggag
     2461 ggctgactca gccgacttcg ggccgcgtca tcattgatgg aactgatttc agcgcattga
     2521 gcgattccga gcgcacaaac atgcgcaaat caaagattgg cttcgttttt cagcggttca
     2581 acctgttgcc gacattgagc gctcttcaga acattgagat tgcctatgaa atctcaggcc
     2641 gcaaggagcc gctcgaccgc aactatcttg accatttgac ggacttgctt ggcatccgag
     2701 gacggcttac gcaccgcccg gctgagcttt ccggaggtga gcagcagcgt gttgccatgg
     2761 cccgcgcgct gattacccat ccggcgatca ttctggccga cgagccgaca ggcaatctcg
     2821 acagtaagaa ctctgaagcg gtgctggaca tgctaatgcg ctcgaaccgt gagctgaacc
     2881 agaccatcct gatgattacg cacaaccctg aagcggctgt gatcgccgac cgtatcctac
     2941 atatgaaaga cggagaagtt gtcagcattg aagccggcaa agggcagggg cgcgtcagct
     3001 atgtcggatt gtagatctgc cttattttgg cggcacctca ggtgtcagca caatttcccc
     3061 caaaattccc gagggcagcg gctccagatg gtccatgtcc tgcgggacga atttctgtcc
     3121 gtagcgcaga ttcagcagac ggtagtcagg gcgtgcctgt ccggcaagcg tgttgatgcc
     3181 ggtattcgcc acctcaattt caacgtggtt cttgccggtg tgcaggaatg atgtgacatc
     3241 gagtctgtac ggaggcgccc agatggagcc ggctttctgc ccattgatgc ggatgatggc
     3301 gatttcgcgc acaggaccct cgagaagagc atgcgtccct ggctttttcg tatcgctagg
     3361 gggcggcagc gggtttcctt cgccaaaatc gagaaagata tgttgccccg gcttgagcat
     3421 ctttgccgga agatcgaagt cacgggtgta agtcgctgtt cctgagtagt agcgtgtgtc
     3481 ttcatccttt gtccaggatg cgaggtgttg catcgtttct gtgcgatgga tgttgtcaaa
     3541 tgagacctgc cagttctggc tcagatccaa ctgcttcgca gtgtccggct ggccgtgcca
     3601 ttctgtgggg agcttttcat catcatcaca aaagataaag gcgcgtgatt catacggcgc
     3661 aagcgaaatc gccgtatcac gggtattggc ccgatagcgc ttgccgtaga gagcatccca
     3721 ttcctctgca aatttccatt gcgtacggaa gcgtgccgtc gttgtcacgc gatgattgct
     3781 ggtgtttgcg acaaagtaga tatctgcatt cggcagtttg cgatgaatga agccgatctc
     3841 cggagccgga gtggcaagct gcatgtccgg cagaagaagt tttctgagct gcgtgccggt
     3901 ttcggattca ggaattgcgc tcgggaagag tttctgcgaa attgcagcga tttgcggaga
     3961 atctgcatta aactcgcgca aacccggagc gtgctccgga agtctgccca cagcaatgac
     4021 cttgccgccc ttttgcgcat actctgctat gacgcgatag accgtaagcg gaatccggtc
     4081 tacatgcggc agaatcagca ccggatactg aatacccagc ttctgaatcg ccgcatcatc
     4141 tatgtagtcc aggttatagc cagcatcgag aatacttgct gtcagctctg gtgtgacgta
     4201 tttgcggatg tcatcggcca agtcaacgtg cccgggagtg aaattggcct gcgcgtcatc
     4261 ggtaggcaga taaacagcga catcattggc aggcttgcct gccctcagca aaaagctcac
     4321 gcgttgaaga tactgcgtga tctccggcat gacgggccac cacggattgt ggtcattaaa
     4381 gacagcggca gcgtaaaaag agtatcccgg atctccggca tatggtggtg aatagggcca
     4441 gccgtggccg acaaactggt tgactccttc gagaaagaag aggtcagctt cagcctttat
     4501 gtcgagagga acggcacgaa atgcaggcga gtgcagccat gtccacgctt cggctgaggt
     4561 gacggggcgg ccgtacaaat ggctggccga agtggcccat cgcgtatagg agaagctgcg
     4621 ccattgcggg ccttctcctt cgggcaggtc tacaaggtta ttgctggaaa gcgtgacagc
     4681 gggcgctccg tacgtctgcg agcggaagag agtatggtgc tggtgcgccc atgcggtaat
     4741 gggctgaaga taattctcgt caattaattc gcagagggtc tggccccagt catggcgcac
     4801 gctctttgcg gtttcatcgt tgccgaaaaa gagttcgggc aggtatggag tcaagtcgta
     4861 tccgcgacgt cttttgaatt gatcgaggaa atcaggcgtc cagtcagagc cgtagacttc
     4921 gagactatcg ctaaaaacgg agtatggcgc ctggtctcca aaggcggtca tcaggcggtc
     4981 gcctacggtg tgcaggtgat ggtctatggc tgtgcggtcg aagtggtcga ggacaaaacc
//
LOCUS       DASRBV010000170         8119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_216871, whole genome shotgun sequence.
ACCESSION   DASRBV010000170 DASRBV010000000
VERSION     DASRBV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216871"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(707..783)
                     /locus_tag="VFA02_06420"
     tRNA            complement(707..783)
                     /locus_tag="VFA02_06420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Pro,seq:cgg)
     gene            1252..2709
                     /gene="sufB"
                     /locus_tag="VFA02_06425"
     CDS             1252..2709
                     /gene="sufB"
                     /locus_tag="VFA02_06425"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZU09523.1"
                     /translation="MGSTVNEIRDLSQQEYKWGFVTEIEAEHVPRGLNEDIIRMISAR
                     KREPEFMLEWRLKAYRYWASLEKSQAEPKWANVRYGPIDYQDIIYYSAPKQKPGIKSL
                     DELDPEILRTYEKLGIPLAEQHRLAGVAVDAVFDSVSVATTFKEKLAEAGVIFCSFSE
                     AVEKHPDLVQKYLGTVVPYTDNFFAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINA
                     AETGQFERTLIIADEGAYVSYLEGCTAPIRDENQLHAAVVELIALDNAEIKYSTVQNW
                     YPGDKQGRGGIYNFVTKRGKCAGVDSKISWTQVETGSAITWKYPSCILQGDNSVGEFY
                     SVALTNNYQQADTGTKMIHIGKNTRSTIVSKGISAGHGQNTYRGMVRIQKGATGARNY
                     TQCDSLLIGDQCGAHTFPYIEVKNSTAHMEHEASTSKIGEDQLFYLQQRGLNREDAVS
                     MIVNGFCKQVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            2720..3481
                     /gene="sufC"
                     /locus_tag="VFA02_06430"
     CDS             2720..3481
                     /gene="sufC"
                     /locus_tag="VFA02_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZU09524.1"
                     /translation="MSLLEIRNLHAEVGGHEILKGIDLTINPGEVHSIMGPNGSGKST
                     LAQVLSRRESYNVTEGEVLFNGKDLLAMKPEEAACEGVFMAFQYPVEIPGISNAYFLR
                     SAVNAVRKYRGLDELDAMDFLPLYRERLKLLEMDEKLMNRSVNEGFSGGEKKRNEILQ
                     MAVLEPKLAILDETDSGLDIDALRIVAQGVNALRSPERSMLVITHYQRLLNYIVPDFV
                     HVLVDGRIVRSGGAELALELEEKGYGWIEAETLSA"
     gene            3494..4873
                     /gene="sufD"
                     /locus_tag="VFA02_06435"
     CDS             3494..4873
                     /gene="sufD"
                     /locus_tag="VFA02_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZU09525.1"
                     /translation="MAVELAQVGSYLETFTAFTQETAPDTPQWLRRLRESAFARFYHA
                     GFPSTRDEDWRFTNLSALTRTSFRLPRKVVLTAADIANWRMKDAAAQIVFIDGQFVPE
                     LFLKGALPEGAAVGGLWQEIASGTKGLTEHLGRYINIERDPFCALNTAFVQDGAYVHV
                     RRGTVVEAPIHLLFISTASETPAMTHPRNLIIVEEQAQVSIVEEYVSFGEETPAFSNA
                     VTELVAGDNANVVHTLIEREHVQTFNFSTLRIEQARSANVTSHSLLLGGGLVRNNVHP
                     VLAGEGGECLINGLFIGSGHQHLDNYMLVEHAKPHCASRQFYNGILDAQSHGVFHGRI
                     IVHKDAQKTDAKQTNRNLLLSDDAQIDTKPQLEIYADDVKCTHGATIGQIEETALFYL
                     RSRGIAETEARRLLLQAFAGECLERLSAGPLRSYAEAAVQERLVALAEAPRAQSSGAI
                     RDLNWEEVQ"
     gene            4870..6120
                     /locus_tag="VFA02_06440"
     CDS             4870..6120
                     /locus_tag="VFA02_06440"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZU09526.1"
                     /translation="MTAQTAVAVPAGYDVEKVRADFSVLREQVHGRPLVYLDNAATSQ
                     KPDAVIDAITNYYRHDNANIHRGVHLLSQRATEEYEAARERVQAFLHARKAREIIFVR
                     GATEGINLVAQTFGRTNVHAGDEVLITALEHHSNIVPWQILCEEKGATLRVAPINQKG
                     ELELDAFEGLLGPRTKIAAISHVSNALGTINPVKQMIEIAHASGVPVLVDGAQAVPHM
                     PVDVQDLDCDFYVFSGHKVYGPTGIGALYGKAELLEVMPPYQGGGDMISSVTFEKTIY
                     NKVPYKFEAGTPDIAGVIGLGAALNYIGRLGIEKIGAHEHELLQYATERIGKMDGVRL
                     VGTARQKAGVLSFVLEGIHPHDIGTILDQEGIAVRTGHHCAQPVMDFFAVPATVRASF
                     ALYNTKQEIEALAQGIEKVKEILG"
     gene            6120..6566
                     /locus_tag="VFA02_06445"
     CDS             6120..6566
                     /locus_tag="VFA02_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZU09527.1"
                     /translation="MAELRDLYQEVILEHSKAPRNYRTLAAATTKAEGFNPLCGDRCT
                     VYLCLKDDVIEDIAFQGSGCAISRASASMMTQSLKGKTTREAEELYERFHGMVTGENG
                     GHSSDVGKLAVFAGVSEFPARVKCATLAWHTFKAALHGEQKPVTTE"
     gene            6578..7162
                     /gene="sufT"
                     /locus_tag="VFA02_06450"
     CDS             6578..7162
                     /gene="sufT"
                     /locus_tag="VFA02_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HZU09528.1"
                     /translation="MMDQIQLNRDTEVIAVPGGQRETLPAGTRVRIVQSRGGSFTVTT
                     ERYAMYRIDGADADALGLAPTGGAGTQIRQQQGPLTEAMVWDTLKTVYDPEIPVNIVD
                     LGLIYSCEITPREDGGKAVSVRMAMTSPTCGMSNVLRADVETRLLRLPEVKETQVEVV
                     FDPPWDRSRMSEAARLQLGLDMEEGGRSGFVRLS"
     gene            7598..8032
                     /gene="rplM"
                     /locus_tag="VFA02_06455"
     CDS             7598..8032
                     /gene="rplM"
                     /locus_tag="VFA02_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZU09529.1"
                     /translation="MATYIPSANEINRKWFVLDANGKTLGRLATEAASVLSGKRNPKY
                     TPFIDMGDHVIVINAEKVRLTGLKSQSKIYRRYTGFPGGLREESFVRLLERKPEKIVE
                     EAIKGMLPKTKLGRKMASKLNVYRGDKHPHQAQKPEVLEVKA"
BASE COUNT         1882 a         2048 c         2373 g         1816 t
ORIGIN      
        1 gccttcttta cctgaccttc ggttttatct tcagcgttgg cttctgggca aagcctctgg
       61 ttaataatgc gacgctgcgt tggaagagca cagacctttc agcagttgtc tgaaaagtag
      121 cgacaaagaa tcagaggttg agagtgcgca aagttcgatg aattgcactt tttctttgag
      181 ggcataacta ccgagcccct ccagtccgcg ataagagtga tttctcacat tgccgaaatt
      241 ctgtcctggc aatctcacgc aaagagtggc atgcgacaaa ataaaggtag attgtctgcc
      301 gctgctctgt gctgctggca aagcaggaca ttcgccgagt gatcgcgatg cgttacccga
      361 gctgacaaaa tccatcgtca cgttgatggt agtgggctca ttgcgagtcg gagaggtcac
      421 cgcgcccccg tggattttcg gagcttccgc acaatacatt cgagcctgat gagcagtgct
      481 ggcgcccggc ccgaagtcac gcgcgaaaac atgggccact cgacggtgga tgtgacgcag
      541 aacgtttata accgcacgtg gtgggaagaa cgagtcgaag ctgtgagttt agccgccgcc
      601 agcgtttggc gggagtttag accgggccag cagcctgccc gatgaaagta aacccttttg
      661 tgtaaaccct cctgccccgg cgattttgca agttgttgaa aagaaatggt cggggagaga
      721 ggatttgaac ctccgacccc ctggtcccga accaggtgct ctaccaggct gagccactcc
      781 ccgatgtttt ttgtgtaagc gtttctggcg aaacggcttt attattcctg gtttttacag
      841 tgtatcagaa catgcgaagc ctttctggga tgaaagcatt ttcacaaatt cagtaccctc
      901 catttgctct atgcagtgtc gccgactcgt tgcgggtcgc gacgcctgta atttcatggc
      961 atggttcaga gtatctatga aaatgctaca gcagcccgcc tgcctggcgc ggattctatt
     1021 gaagatggcg cggcatcatt gacaaccttt tttaggatcg taactttctc ttttttcagt
     1081 cctggccgct acttgtgcat cccatagggt atagagtcgt agtgggaaaa gaagacatct
     1141 tctaatggcg tgtaatttga ttcaaccaaa ttacagaggt atactaggga taatatagga
     1201 ctaatttagt cgtatatcta tcggccaatg agaagacaac aagggggcgc gatgggttct
     1261 accgtcaatg aaattcgaga cctgagtcag caggagtaca agtggggctt cgtcacggaa
     1321 attgaagccg agcacgttcc gcgcgggctg aatgaagaca tcattcgcat gatctcggcg
     1381 aggaagcgtg agcctgaatt catgctggaa tggcggctga aggcgtatcg ctactgggcc
     1441 tcgctggaga aatcgcaggc cgagccgaag tgggccaatg ttcgatatgg gccgattgat
     1501 taccaggaca ttatttatta ctctgcgccc aaacagaagc ctggcatcaa gagcctcgat
     1561 gagcttgacc cggaaattct gcgcacatat gaaaagcttg ggattcccct ggccgagcaa
     1621 catcgcctcg cgggcgtggc tgtggatgca gtgtttgaca gtgtctctgt cgcgacgaca
     1681 tttaaggaaa agctggcgga agccggagtc atcttttgct cattctctga agcggtagag
     1741 aagcatcctg acctggtgca gaaatacctg ggcaccgttg ttccttatac agacaatttc
     1801 tttgctgcgc ttaactcggc ggtctttagc gatggctcct tcgtttatgt accgaagggt
     1861 gtccgttgcc ccatggagct ttccacctac ttccgcatca acgcagcaga gacgggccag
     1921 tttgagcgca cactgattat tgccgatgaa ggcgcttacg tcagctatct ggaaggctgc
     1981 actgcgccga tccgtgatga gaaccagttg catgcagctg tcgtagaact gatcgcgctg
     2041 gacaatgctg agatcaagta ctcgacagtg cagaactggt atccaggtga taagcagggg
     2101 cgtggcggaa tctacaattt tgtgactaag cgcggcaagt gcgcaggcgt ggactcgaaa
     2161 atctcctgga cacaggtgga gaccggctcc gcgattacgt ggaagtaccc aagctgcatt
     2221 ctgcaaggcg acaattcggt tggcgagttc tactccgtgg cgttaaccaa caactatcag
     2281 caggctgata cgggcacgaa gatgatccac attggtaaga acacgcgcag cacgattgtg
     2341 tcgaagggca tctcggctgg gcacgggcag aacacgtatc gcggcatggt gcggatccag
     2401 aagggcgcta cgggcgcgcg caactatacg caatgcgatt cgctgctgat tggcgaccag
     2461 tgtggcgcgc acacgtttcc ttacatcgaa gtaaagaact cgacagcgca catggagcac
     2521 gaggcctcga cctcgaagat tggtgaagac cagctctttt atctgcaaca gcgtggattg
     2581 aaccgtgaag atgcggtttc tatgatcgtg aatggattct gcaagcaggt ctttcgtgag
     2641 ctgccaatgg agtttgctgt ggaagcgcag aagctgttgg gcgtgagcct ggaaggcagc
     2701 gtgggttgag gagacaggga tgagtctgct ggaaattcgt aatctgcacg ctgaagtagg
     2761 cgggcatgaa attctgaagg gcattgactt gacgatcaat cccggcgagg tgcattccat
     2821 tatggggcca aatggctcag gcaagagcac gctggcccag gtactttcgc ggcgcgagtc
     2881 ctacaacgtc acagaaggtg aagtgctctt taacggcaaa gaccttctgg cgatgaagcc
     2941 ggaagaagct gcctgtgaag gcgtgtttat ggcctttcag tatccggtgg agattccggg
     3001 catcagcaat gcatattttc tgcgcagcgc agtgaatgca gtccggaagt atcggggcct
     3061 cgatgagctg gacgcgatgg acttcctgcc gttgtaccgc gagcggctta agcttctgga
     3121 aatggatgag aagctcatga accgctcagt gaatgaaggc ttctccggcg gtgaaaagaa
     3181 gcggaatgaa attctgcaga tggcagtgct cgagccgaag ctcgctattc ttgatgaaac
     3241 cgactccggt cttgatattg atgcattgcg catcgtggcc cagggagtga atgcgcttcg
     3301 cagtcctgag cgttccatgc tcgtcattac gcactaccag cgcttgctga attacatagt
     3361 gcctgatttt gtgcacgtgc tggtagatgg gcgcattgtg cgttcaggtg gagcggagct
     3421 agcactggag ctcgaagaaa aaggttacgg ctggattgaa gcggagacct tgtccgcctg
     3481 aagggagtga acgatggctg tggaactggc ccaggttgga agttatcttg aaacctttac
     3541 cgcgttcacg caagaaaccg ctccggacac accgcaatgg ctgcgtaggc tgcgggaaag
     3601 cgcctttgcg cgtttttatc atgctgggtt tccttccaca cgcgatgaag attggcgctt
     3661 tacgaacctt tctgcactga cgcgaacatc ttttcgtcta ccgcgcaaag tagttttgac
     3721 cgccgcagac attgccaact ggcgcatgaa agatgcagcg gcccagattg tttttattga
     3781 cgggcagttt gttccggagc tgtttctgaa gggcgcgctg cctgagggcg cggctgtcgg
     3841 cggattgtgg caggaaatcg caagcgggac aaagggtttg accgagcacc tgggccgtta
     3901 catcaacatc gagcgcgatc cgttctgtgc gcttaatacg gcctttgttc aggacggagc
     3961 ttacgttcat gtgcggcgtg gcacagttgt agaagcaccc attcatcttt tatttatttc
     4021 gaccgcgtct gaaacccctg cgatgacgca tccgcgcaac ctcatcatcg tagaggagca
     4081 ggcgcaggta tcgattgtgg aagaatacgt ttcgtttgga gaagagaccc cggcattctc
     4141 gaatgcagtt acagagcttg tcgcgggcga caacgcgaac gtggtgcata cgctgattga
     4201 gcgcgagcac gtgcagacct tcaatttctc gactctgcgc attgagcagg cgcgcagcgc
     4261 caatgtcaca tcgcactcat tgttgctggg tggtggattg gtacgcaaca atgtgcatcc
     4321 tgtgcttgcg ggcgaaggcg gcgagtgcct gatcaatggc ctgttcattg gcagcggaca
     4381 ccagcacctc gacaactaca tgttggtgga acatgcgaag ccgcattgtg ccagccgcca
     4441 gttttacaac ggcattctcg atgcgcaatc gcacggcgtt tttcacggcc gcatcatcgt
     4501 ccataaagac gcgcagaaga ccgacgcaaa gcagaccaac cgcaatcttc tgctttcaga
     4561 tgacgcccag attgatacca agccgcaact ggaaatctat gcagacgacg tgaaatgcac
     4621 gcacggggca accattgggc agattgaaga aaccgccctg ttctatctgc gctcgcgcgg
     4681 cattgcagaa acagaggcgc gcaggcttct tttgcaggcc tttgccggag agtgcctgga
     4741 acgtctgagt gctggcccgc tacgctcgta tgctgaagct gccgtgcagg agcggctggt
     4801 cgcgcttgcc gaagcgccgc gcgcgcaaag cagtggtgca attcgtgacc tgaattggga
     4861 ggaagtgcaa tgactgcgca aactgcggtt gctgtgcctg ccggctatga cgtggagaaa
     4921 gtccgcgctg atttttccgt gttgcgagaa caagtccacg gacggcctct tgtctatctg
     4981 gacaacgctg caacatcgca gaagccggat gctgtgattg acgcgatcac gaactattac
     5041 cgtcacgaca acgcgaatat tcaccgtggt gtgcatctgc tttctcagcg tgcaactgaa
     5101 gagtacgaag ctgcgcgcga gcgcgtacag gccttccttc atgcgcggaa agcacgcgaa
     5161 atcatctttg tgcgcggggc cacagagggc atcaaccttg ttgcccagac gtttggacgc
     5221 accaatgtcc atgctggcga tgaagtcctg attacggcac ttgaacacca ttccaacatt
     5281 gtgccctggc agattctctg tgaagaaaag ggggcgaccc tgcgcgttgc tcctatcaac
     5341 cagaagggcg aactggaact ggacgcattt gaagggctgc ttgggccccg cactaagatc
     5401 gctgcgattt cacacgtctc caatgcattg ggaacgatca atcctgttaa gcaaatgatt
     5461 gaaattgcac atgcatcggg tgttcctgtg cttgtagatg gagcgcaggc ggtgccgcat
     5521 atgccagtgg atgtgcagga cctcgattgc gatttttatg tcttttcagg gcacaaagtt
     5581 tacgggccga ctggtattgg agcgctctac ggcaaggcgg agctactcga agtgatgcct
     5641 ccgtaccagg gcggcggaga tatgatcagc tccgtcacct tcgagaagac catctacaac
     5701 aaagttccat ataagtttga agcggggacg ccggacatcg caggcgtgat cggtctcgga
     5761 gcggccctta attatattgg aaggctcggc attgaaaaga ttggcgcgca tgaacacgag
     5821 ctgcttcagt atgcaactga acgcatcggc aagatggatg gcgtgcgtct cgttgggaca
     5881 gcgcggcaga aggccggcgt cctttccttc gtgctcgaag gcattcaccc gcacgatatc
     5941 ggcacgatcc ttgaccagga gggcattgcc gtccgaacag gacaccactg tgcacagcct
     6001 gtgatggact tctttgcggt ccccgcgacg gtgcgcgctt ctttcgccct ctacaacaca
     6061 aagcaggaaa ttgaagcgct ggcgcagggc atcgaaaaag tgaaggagat tctgggctga
     6121 tggctgagtt gcgcgatctg tatcaggaag tgatccttga acattcaaag gccccgcgta
     6181 attaccgcac gctggctgca gccaccacca aggccgaagg gtttaatccg ctctgtggag
     6241 accgttgcac cgtctatctc tgcttgaaag atgatgtgat tgaagacatt gcttttcaag
     6301 gctcaggatg cgccatctcg cgcgcatcgg cttccatgat gacacaaagt ctcaaaggaa
     6361 aaaccacgcg cgaggcggaa gaactgtacg aacgttttca cggaatggtg acaggagaaa
     6421 acggaggcca ctcttcggat gtgggcaagc tggctgtgtt tgccggagtc tctgaatttc
     6481 cggcgcgcgt aaagtgcgcc acgctggcat ggcatacatt caaggccgcg ctgcatggcg
     6541 aacaaaagcc agtcaccacc gagtagcagg tttattcatg atggaccaga ttcaactcaa
     6601 tcgcgatact gaagtcattg ccgttccagg cggccagcgc gaaacgcttc ctgctgggac
     6661 tcgagtccgc atcgtgcaat cgcgcggcgg cagcttcacc gtgacgacag agcgatatgc
     6721 catgtaccgc atagatgggg cagacgccga tgctctcggg cttgcgccta ccggaggtgc
     6781 tggcacccag atccggcagc agcaaggtcc gctgacggaa gcaatggtat gggacacact
     6841 gaagactgtc tacgatcccg agattccggt gaatatcgtg gatctcggcc tcatttactc
     6901 ctgtgaaatt acaccacgtg aagatggcgg caaggcagtc tctgtgcgca tggccatgac
     6961 ctctccgacc tgcggcatga gtaacgtgtt gagggcagat gttgaaacca gattgctgcg
     7021 actgcctgaa gtgaaagaga cccaggtcga ggtggtcttc gatccaccct gggaccgcag
     7081 ccgcatgtca gaagctgcac ggcttcagct cggtcttgat atggaggagg gcggccgttc
     7141 cggattcgtt cgtctatctt agttctccat tatggtcggg ggctggtgcc accgtgcaca
     7201 aagaccgcat ctgcaaaaag atgcgccttg tcccatggtg gcggtacggt ggccattggc
     7261 agtttctggg acctctctct gaaagacccc aggtccccaa aaactctatg aggccagccg
     7321 acatggcccg ggagcggcca gaaatcggcg gaagaccaag ttgcgaattg acttcgccct
     7381 caaaatccat tactctaaaa tccttgcgca gtcccgcgct tgcctgtctg tatgtctttg
     7441 aggctgggct ctttcgagcg ggaaaaccca atcggttgta actgcgcacc ctacgcgaac
     7501 agatgatgtc ctgccgtggg aattgaatgt taccgcggtg acgccttgcg tgggcgaaga
     7561 gcgtttcaac gcagagattt gctggagata agtaaagatg gcgacgtata ttccgagcgc
     7621 gaacgagatc aaccgcaagt ggttcgtgct ggacgcaaac ggcaagaccc tgggacgtct
     7681 ggcgactgaa gcggccagtg tgctcagcgg taagagaaat ccgaaatata cgccctttat
     7741 tgatatgggc gaccacgtaa ttgtgatcaa tgcggagaag gtaaggctga ccggactgaa
     7801 gagccagagc aagatttacc gccgctatac cggtttcccg ggcggtctgc gcgaagagtc
     7861 atttgtccgc ctgcttgagc gcaagccgga aaagattgtg gaagaggcga tcaagggcat
     7921 gttgcccaag acaaagctgg gccgcaagat ggcgtccaag ctgaatgtgt atcgcggcga
     7981 caagcacccg catcaggccc agaagcctga ggtgctggaa gtaaaggcat aggattcagg
     8041 tttaaaggat aacaatggca gatttggtcc agtattacgg aacgggacgg cgcaaggcgg
     8101 caattgcccg cgtttttct
//
LOCUS       DASRBV010000171         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_62994,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000171 DASRBV010000000
VERSION     DASRBV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62994"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="VFA02_06460"
     CDS             complement(<1..1648)
                     /locus_tag="VFA02_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZU09530.1"
                     /translation="MPSRTRRTVFALVVFFSACGLAGLVVSQKVGAQSSNDESQFRDS
                     LKTFTNVYDIVAQNYAEPLTGDKPNQVIYDGAIPEMLHTLDPHSSFYDPKAYAAMKEE
                     QHGKYYGVGMVIQPQLINGVQKVVVIYPYEGTPSYRAGVRPGDIIVSVDGKSTDSMAS
                     DQVANMLKGPKGTHVKLTVSREGVAAPITFDLVRDEVSHPSIDLKEWIRPGIGYIHIT
                     QFQETTGREVSDALDSFGPDLKGLLIDLRANPGGLLTEAVATCDKFLKKGQVIVSQRG
                     RAYPEQTYHAMHGNDGRTYPIVVLVNHGTASAAEIVSGALQDHDRALIVGETTFGKGL
                     VQTVYQLSDNTGLALTTYHYYTPSGRLIQRNYSGVSLYDYYYNHDQPQDNKDREVKLT
                     DSGRTVYGGGGITPDVKIDTPKSTHFQEVLMSPLHYSFFNFARHYLSDPNHKVTRDFQ
                     VDDAVLNDFKQFLGTQNITFTDQEFNSALDWIKTNIKAEIFTTEFGQMEGMKVRAAWD
                     PMINQALTYMPQAASLEQAAIKADSQKTATAKNTPSVPASE"
     gene            2057..3838
                     /locus_tag="VFA02_06465"
     CDS             2057..3838
                     /locus_tag="VFA02_06465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU09531.1"
                     /translation="MNFRFLPLPAAFIAGIVLSTAPSFAQTQSKPSAPSQDLPYQGTV
                     VERIIARVNDQVITNSDYQRARQELEAQARQQGWTQQQLFEQEHNLLRDLIDQQLLLS
                     KGKELGISGETETVRRLDEIRKQNHLDSMEDLQKAAEAQGVSFEDFKQHIREGVISSM
                     VIRDEVGRHLSVSQSEVQQYYEQHKADFDQPEQVRLSEILIPTPNPDDNAQVETAQKK
                     ADDIEAKLKSGADFATLAKAESGGPTASQGGDLGEFKRGQLAKVLEDQTFPLKPGEYT
                     QPIRTKQGFVILKVTEHTAGGVQPLKDVQPQVEDALYQQKMAPALRQYLTKLREQAYI
                     EIDTKDGYEDSAATANETQPTTTYSAYVPPIKKKKHVERTRFRQKGPKKQPQTQTAST
                     PAPANAPSLAQVPGAATAEANGQQPAAAQPATAPAQKNTQTASVGVEKPGKKEKIRFG
                     QAPRETLPYAPTKTEDAGAGTETATNVAQNVQVVGPNGEVENQQPQEKKEKSRFSDRA
                     RQPKQKKSKDKTDPFAPAPETTDEQATRQAQSAPLGLNGDTSKPKKEKPTGKTRLSDK
                     AKEKSTDQQQEQPQPAQQPPSPSSQKQ"
     gene            3852..4922
                     /locus_tag="VFA02_06470"
     CDS             3852..4922
                     /locus_tag="VFA02_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZU09532.1"
                     /translation="MKELFIQDLAKLENQVIIGFFAVAQKQSRSTRDGSCYYAITISD
                     RTGQIECRIWDEAAIVAGFEQGDVVKVQGQVCRYQEKLQLKIEKVRKAKPEEYDLGDF
                     VPKTERDIEQLWAELNSYVDSFTNPHLHALLRAFLDDPETAAALKSAPAAKAMHHAWI
                     GGLLEHIVSLMGVASDMANRYPEINRDILLAGVMLHDIGKLRELRWGTNFDYTTEGHL
                     VGHITIGVRMVEQKIAALPGFPEELRIVVEHMIVSHHGRYEFGSPKLPMLPEAVMLHY
                     LDDLDAKMQTMRSEFVRAEANGRAPGQMTEWVRAMDRPLLNTAQYLAPKPEEKQPQTA
                     SEAVPEENDALPAQDGTGNVLL"
     gene            4879..5259
                     /locus_tag="VFA02_06475"
     CDS             4879..5259
                     /locus_tag="VFA02_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZU09533.1"
                     /translation="MHYLLKTEPETYSFSDLEREGETVWDGVTNPVALKFLGNMKPGE
                     KLVVYHTGDERTAVGTAIVVSADPDNPKSPVVRIKAGSKIKTPRTLSEIKSNKLFADS
                     PLVRQGRLSVVPLNEAQYAWLTGE"
BASE COUNT         1306 a         1574 c         1471 g         1062 t
ORIGIN      
        1 actccgaagc cggaacggag ggtgtgttct tcgcggtcgc agtcttctgc gaatctgcct
       61 tgatggcagc ttgttcgagc gatgctgcct gcggcatgta ggtgagagcc tggttgatca
      121 tcgggtccca ggcagcacgg actttcatgc cttccatctg gccaaactcg gttgtaaaga
      181 tttccgcctt gatgttcgtc ttgatccagt ccagagcgct gttgaattcc tggtcagtga
      241 atgtaatgtt ctgtgttccc agaaactgtt tgaagtcatt gagaacggca tcatctacct
      301 ggaaatcgcg tgtgaccttg tggttcgggt cagacagata gtgccgcgcg aaattgaaga
      361 acgagtagtg cagcggagac atcagtactt cctgaaagtg cgtggactta ggtgtatcta
      421 tcttgacgtc cggcgtgatg ccgccgcccc cgtaaaccgt ccgtccggaa tccgtcagct
      481 ttacttcgcg gtccttgttg tcctgcggct ggtcgtgatt gtagtagtag tcgtacagcg
      541 aaacgccgct gtagttgcgc tggatgaggc gtccgctggg cgtgtagtaa tgatatgtcg
      601 taagcgcaag accggtgttg tcggaaagct gataaacggt ctgcaccagg cccttgccga
      661 aagtcgtctc gcccacaatc agtgcacggt catggtcctg cagggcccca ctgacaatct
      721 ctgccgccga tgctgttccg tggttcacca gcaccacaat gggataggtc cggccatcat
      781 ttccgtgcat ggcatggtaa gtctgctcag gataagcgcg cccgcgctgg gagacgatga
      841 cctggccttt tttcaggaac ttgtcgcatg tggcgaccgc ttctgtgagc aggccgccgg
      901 gattcgcgcg cagatcaatg agaaggccct tcaaatcagg gccgaagctg tcaagcgcgt
      961 cagaaacttc acgtcctgtg gtctcctgaa attgcgtaat gtggatgtag ccgatgcccg
     1021 gccggatcca ctccttgaga tcaatgctgg gatgcgaaac ttcatcccgc actaggtcaa
     1081 atgtaattgg ggcggcaacg ccttcgcggg agaccgtcag ctttacatgc gtccctttcg
     1141 gccctttcag catgttggct acctggtctg aagccatcga gtcggtactc ttgccgtcta
     1201 ccgaaacgat gatgtcgccc ggacgaacgc cggcccggta cgagggcgtc ccttcatagg
     1261 gataaatgac aacaaccttc tgcacgccat tgatcagttg cggctggatc accatgccta
     1321 cgccgtaata cttgccgtgc tgctcttcct tcatagccgc ataggccttg gggtcataga
     1381 agctcgagtg cggatcgagc gtgtgcaaca tctccggaat ggcgccgtcg taaatcacct
     1441 gattcggctt gtctccggta agtggctcgg cataattctg cgcgacaata tcgtagacat
     1501 tggtaaaagt cttcagacta tcgcggaact gcgactcgtc attgctggac tgcgcgccaa
     1561 ctttctgact gacaacaagg cctgcaagac cgcaggcgga aaagaaaact acgagtgcga
     1621 aaactgtacg gcgagtgcgg gaaggcatgt atcctgggac cctcgaggtg tcctggggaa
     1681 agtatagcaa gtcggcgggt cagcaagtca gcgcgcccta cgggcgctgt tagctcaaaa
     1741 gcagtagcgg ctggcgcgcc tttttcggtg ctgttggctt gagaatgcct ttcgtcagca
     1801 tttcaagtta acttccggat gtaaaaccgt tactacaaaa ccgctaacca cttcttagct
     1861 acccgcctct tagccgaccc agcgctaaca acctccctgt acgccacgtc caaaaacctg
     1921 actggttttt gctgactcgc tggccttact ctggcccgct gactcgcttc tgtttagaat
     1981 aatcagcagg cgcaaccgga acgttgagga gcagacccca aaatcgcggc gcgcacccga
     2041 attgagacgt aaaaccatga atttcagatt tttaccgctc ccagccgcgt tcatagcggg
     2101 catcgtatta tctaccgcac cgtctttcgc ccagacccag tccaaaccat ctgccccttc
     2161 gcaggacctg ccttatcagg gcacggtggt ggagcgcatt attgcgcgcg tcaatgacca
     2221 ggtcatcacc aactctgact atcagcgcgc gcgccaggaa ctggaagccc aggcccgtca
     2281 gcaggggtgg acccagcagc agcttttcga gcaggagcac aatcttctcc gcgacctgat
     2341 tgaccagcaa ctgctgcttt ccaaaggcaa agagcttggc atcagcggag aaacagagac
     2401 cgtcaggcgc ctggatgaga ttcgcaagca aaaccacctt gacagcatgg aagacctgca
     2461 aaaagccgcc gaggcgcaag gtgtttcctt tgaggacttc aagcagcaca tccgcgaagg
     2521 cgtcatctct tccatggtca tccgcgatga agttggccgc catctttccg tcagtcagtc
     2581 tgaggtgcag cagtattatg aacagcacaa agctgacttt gaccagcctg aacaggtccg
     2641 cctcagcgag attctcatcc ccacgccgaa ccccgatgat aatgcccagg tagaaaccgc
     2701 gcagaagaag gcagatgata tcgaagcaaa gctgaaatca ggtgctgatt tcgcaacgct
     2761 ggccaaggcc gaatccggag gcccgaccgc ctctcaggga ggagatctgg gtgaattcaa
     2821 gcgcggtcaa ttggccaagg tcctcgaaga ccagaccttc cctctgaagc cgggtgaata
     2881 cacgcagccc atccgcacca aacagggctt tgtcattcta aaggtcacag aacacactgc
     2941 cggaggcgtc cagccgctga aagatgtgca gccgcaggtg gaagacgctc tctatcaaca
     3001 gaagatggcc cccgccctgc gtcaatatct gacaaagttg cgcgagcagg cttacatcga
     3061 gattgatact aaggacggct acgaagactc tgccgccacc gcgaatgaga cgcagccaac
     3121 caccacttac agtgcctacg ttcctcccat caagaagaaa aagcatgtag agcgcacccg
     3181 tttccgtcag aagggcccaa aaaagcagcc gcagacccaa acggcaagta cgcctgctcc
     3241 tgccaatgcc ccgtcacttg ctcaggttcc gggcgcagcc accgcagaag caaatggtca
     3301 gcaaccggct gccgcgcagc cggccactgc tccggcccag aagaacaccc agacggctag
     3361 cgtcggcgta gagaaacccg gcaagaaaga aaagatccgt tttggtcagg ccccacgcga
     3421 gaccctgcct tatgctccta ccaaaaccga agatgctggc gcgggcaccg agactgccac
     3481 caatgttgcc cagaacgtgc aggtggtcgg gccgaatggc gaagtagaga accagcagcc
     3541 gcaggagaaa aaagaaaaat cgcgcttctc tgaccgtgcc agacagccaa agcaaaaaaa
     3601 gtccaaagac aagaccgatc cgtttgctcc tgctccggaa acaacagacg aacaggccac
     3661 gcggcaggca cagagcgcgc ctctcggcct gaatggcgat acctccaaac cgaagaagga
     3721 aaagcccaca ggaaagacgc gtctttctga caaagcaaaa gagaagtcca ccgaccagca
     3781 gcaggaacag ccccaaccgg cccagcagcc gccttcgcca tcttcgcaga agcaataaac
     3841 tggcggacgc tatgaaagag ctctttattc aggacctggc gaaactggaa aaccaggtca
     3901 tcatcggttt cttcgccgtt gcgcagaagc agtctcgcag cacgcgcgac ggctcgtgtt
     3961 attacgcaat caccatcagt gaccgcaccg gccagatcga atgccgcata tgggacgagg
     4021 ccgccatcgt tgccggattc gagcagggcg atgtggtcaa agtgcaggga caggtctgcc
     4081 gctatcagga aaagctccag ctaaagatag agaaggtccg taaagcgaag cctgaagaat
     4141 acgacctcgg cgattttgtt ccaaaaaccg aacgcgacat cgaacaactc tgggcagagc
     4201 tgaacagcta cgttgacagc ttcacaaacc cacacctgca cgccctgctg cgcgcttttc
     4261 tggatgaccc tgagaccgct gcggccctta aatccgcccc tgcagccaag gccatgcacc
     4321 atgcctggat cggcggcctt ctcgaacaca tcgtctcgct gatgggcgtg gccagcgaca
     4381 tggcaaaccg atatccggag atcaaccgcg acatcctcct cgccggcgtg atgctgcacg
     4441 acatcggaaa gctgcgcgaa ctgcgctggg gcacaaattt cgactacacc accgagggcc
     4501 atctcgtagg ccacattacc atcggtgttc gcatggtcga gcagaagatc gccgcattgc
     4561 ccggctttcc cgaggaattg cgcatcgtgg ttgagcacat gatcgtgagc catcatggca
     4621 gatacgaatt cggctcacca aaactgccga tgctcccgga agccgtgatg ctgcattacc
     4681 ttgacgacct cgacgccaag atgcagacca tgcgcagtga atttgtccgc gcggaagcca
     4741 atggacgcgc gccggggcag atgacagaat gggttcgcgc catggaccgc ccgttactga
     4801 acaccgcgca gtatctggcc ccgaaaccgg aagagaaaca gccacagacg gcatcagaag
     4861 cagtgccgga ggaaaacgat gcattacctg ctcaagacgg aaccggaaac gtactccttt
     4921 agcgacctcg agcgcgaagg cgaaacggtg tgggacggcg tcacgaaccc tgtcgccctg
     4981 aagtttctcg gtaacatgaa accgggcgaa aagctggtcg tttatcatac cggcgatgag
     5041 cgcactgcgg taggcacagc gatagtcgtc tctgcggatc ccgacaaccc aaagtcccct
     5101 gtcgtccgca tcaaagccgg aagcaaaatc aaaactccgc gcactttatc tgagatcaag
     5161 tccaacaagc tgtttgccga ctcgccgctt gtccggcagg gcagattgtc cgttgttccg
     5221 ctgaatgaag cgcagtatgc gtggctcacc ggagaatagg cccgatttcg gggctgttcc
     5281 ggaatgggag caagtttggt tcccgtgagc aacttttctc agcattgtta catatctatt
     5341 gcatactgaa agttggagga gtccttgccc cgcatccact ttcagcaaca gctggccgaa
     5401 ctgaaggaca aaa
//
LOCUS       DASRBV010000172         7500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_518543, whole genome shotgun sequence.
ACCESSION   DASRBV010000172 DASRBV010000000
VERSION     DASRBV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518543"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..80)
                     /locus_tag="VFA02_06480"
     CDS             complement(<1..80)
                     /locus_tag="VFA02_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_282916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZU09534.1"
                     /translation="MKGIVLAGGSGTRLYPVTHVVSKQLLP"
     gene            complement(80..1174)
                     /gene="rfbB"
                     /locus_tag="VFA02_06485"
     CDS             complement(80..1174)
                     /gene="rfbB"
                     /locus_tag="VFA02_06485"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZU09535.1"
                     /translation="MQTAKNRSGPILVTGGAGFIGSNFVLEWIRHVGSPVINLDNLTY
                     AGNRENLRTLEGDSRHIFVHGDIADEKLVASLLREHRPRAVVHFAAESHVDRSIVGPD
                     AFIQTNVFGTFALLQAARAYFMELGAVEQKQFRFLHVSTDEVYGSLGPHDPAFTEETA
                     YAPNSPYAASKAASDHLVRAYFHTYGLPVLTTNCSNNYGPYQFPEKLIPLMILNGLEG
                     KPLPVYGDGRNVRDWLFVEDHCSAIRTVLEHGKLGETYNIGGNSERANIDVVTTICDL
                     LDELAPDQKTGSRRKLITYVKDRPGHDRRYAINAEKIRRELGWTPAEKFETGLRKTVQ
                     WYLDHREWVENVRSGAYLKWIETNYAGRTA"
     gene            complement(1224..2354)
                     /locus_tag="VFA02_06490"
     CDS             complement(1224..2354)
                     /locus_tag="VFA02_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZU09536.1"
                     /translation="MSTSTSSLQFDSAANWKDEVNARLAAHRTRRTRVPESQTALPGF
                     DLRHAEGGRKSVAERVAERYSKAPSYQELLAAQAAAARAAEAAAEAAMQAHAATQAAL
                     AGLEAHEEEDSGAAQPGPVAETPHVMVAEAADPFEEVTVTPPQPLPAKLIEFPRELIA
                     PRKARPRLAEGPLREADPGPEGSQLRIFEVEPEDISKDPVVEPVLPEWHSIRLDAKPA
                     EEMQPAEAAAPAANYSHLFDLPLKVASMEDRLMAGIVDTALVLASFFVFVLVFVACTA
                     HPPTGKPAIAGASIALGVLFVLYQWLFFTYSDATPGMRYAKIALCTFDDENPTRKMMR
                     NRVGALLLSALPMGLGFLWSFFDEDHLGWHDRITRTYQRSYR"
     gene            complement(2323..2460)
                     /locus_tag="VFA02_06495"
     CDS             complement(2323..2460)
                     /locus_tag="VFA02_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09537.1"
                     /translation="MSAASARAWLGIGFGVSLLPPLLEHIGLLTFTGNSIEHFDLEPA
                     I"
     gene            2554..5007
                     /gene="lptD"
                     /locus_tag="VFA02_06500"
     CDS             2554..5007
                     /gene="lptD"
                     /locus_tag="VFA02_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZU09538.1"
                     /translation="MRARIFLCITLLCLCHPQLWPQAVTNEFPPPSQLPQLPDAPDVP
                     VARVVPAPPGGVPVEIRAISQREKDNVYTLEGNVVIHYRDYVIQADKIVYNRDTGEVV
                     ADGHLTLDGSPDNVHLTATHGQMNVDADTAHFYDVIGTIGSGSERTLPRNKRVFTSPN
                     PFAITGRELIKLGPGSYKVIDGTMTSCHLPSPDWRLLAKEISVDNGTATARNTVFQLL
                     HVPLLYLPYATHAVNEEQRTSGILLPIIGNSTTKGFIFGEQVYFALGRNSDLTLGTEY
                     FSKRGFSPMGLFRYRGFGEDFFNVRFHSLLDRGLPPNHIDQGGVDLMIDGRRDWGSHT
                     RGIVDAEYLSSYIYRQAFEENYATAIDSEVKSQLFGVHDLNGYSQSLRLDRYQSFQNT
                     STNAEIRLLHMPELRLEAEDHYLGNDTSLMWGGIASVAALSRSEPANSLNTATFRAHF
                     VPRVDLYPHIALPFHLDGWSFRPEAAVRETFYGKSQTPAPLGTVPSLRNASVERKDVE
                     AGIDVRPPAIERDFSAPWITRLFGGDLRHSIEPDIQYHYVAGISKFDSILRFDDVDIA
                     SDTSELDYSLTQRLFRRHLHPHPCKGDEALGPDDLCGGGSVDWLSWQLAQKYFFNNTF
                     GGAVTTGTRNVLATTLDLTGIAFLTGPRTFSPITSRLRMRTSSATDLEWDLDYDPRLG
                     RITASNVYAGYKFGDYNFNLGEFHLNAPEAPATVGQGAQTTSIISNYEQLRLTASYGS
                     STKAGLSAGGTLGYDFVQNQLQYGAIQSAWNWDCCGLSLEVRRYSLGAVRDDTQYLYS
                     FTLAGVGSAGSLRRAVRIF"
     gene            5011..5625
                     /locus_tag="VFA02_06505"
     CDS             5011..5625
                     /locus_tag="VFA02_06505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU09539.1"
                     /translation="MDIKAHWQGIYGTKSPDSVSWYAPHLEMSLRLINHANLSRDAYI
                     LDVGGGASTLADDLLTQGYKHIAVLDISEAALNISRKRLGEQAGRIRWIAGDVTSTQL
                     QEHAYDLWHDRAVFHFLTTPEQRAAYIRQLLHTLKPGGHLVLATFASCGPERCSGLEV
                     VRYSTDSLARELGENFYLMESAEEDHITPSGAKQPFLYCRFQIV"
     gene            5678..6631
                     /locus_tag="VFA02_06510"
     CDS             5678..6631
                     /locus_tag="VFA02_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU09540.1"
                     /translation="MLRTRFFFGCLALLLAAGCKAQDNPNDLNNAKIDRRIEVLVRSQ
                     LNVPQDWKVNIGPRSKSDVPGFDAVPITFSSMSDSSKQQTLNFLLSKDGNTLARLSKW
                     DLSRNPADLISTANRPVRGNPDAKVTIINFDDLECPYCARMHAQLFPDTLDRYKGLIK
                     VVYKDFPLVEIHPWALHAAVDANCLAAQSPTAYWNYVDYLHTHGDDVSGPQRDPAKSA
                     ATLDKLARDEGQRSKLDSGKLDACLAKQDDSVVRASMKEGDALGVDGTPTLFINGERL
                     SGALPEEQVWMAIDRALKAEGITPPAPEKPTVPAAGKGLGK"
     gene            6716..>7500
                     /locus_tag="VFA02_06515"
     CDS             6716..>7500
                     /locus_tag="VFA02_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZU09541.1"
                     /translation="MLLRVLTVPRNSSAFLPRFQQMAMVCVASLAMAGAAGCHRTHGP
                     DVVATVNGKPIMRSEVEQLYQNNLGDSQQQPSKEQADIVRLNIIRQLIDEEILMQRAA
                     KLNLTATDEEVDNQLNELKAPFTQEEFNKRLADKHLTLDDLKRQIRRSKTEDKLLNKE
                     INSKINITDADISSYYNAHKSEFNLIEPMYHLAQIIVTSQPLPQQQQTSNLQNSKATN
                     DAEAKKKIEMLHNRLESGEDFGLLAQNFSERPDTASNGGDMGFV"
BASE COUNT         1627 a         2369 c         2000 g         1504 t
ORIGIN      
        1 ggaagcagtt gcttggaaac gacatgtgtc accggataga ggcgcgttcc cgaaccgcct
       61 gccagcacaa tccccttcat catgcagtcc gccccgcata gttggtttca atccatttga
      121 gatacgcacc cgaacgcaca ttctctaccc attcgcggtg gtccagatac cactggaccg
      181 tcttgcgcag cccggtctca aatttctctg ccggcgtcca gcccagctcg cgccggattt
      241 tttctgcgtt gatggcataa cggcggtcgt gcccgggacg gtctttcaca taggtaatca
      301 gcttgcgacg gcttcccgtc ttctggtctg gcgcaagctc atccagaaga tcgcagatag
      361 tcgtgaccac gtcaatgttg gcccgctcgc tgttgccgcc gatgttgtac gtctcgccca
      421 gtttgccatg ttccagcacg gtgcgaatgg ccgagcagtg gtcttctaca aaaagccagt
      481 cgcggacgtt cctgccatct ccatagacgg gaagcggctt gccttcaagt ccgttcagaa
      541 tcatcaacgg aataagcttt tccggaaact gatacgggcc atagttgttc gagcagttcg
      601 tcgtgagcac gggcaggccg tatgtgtgaa agtacgcccg caccaggtgg tcagaagctg
      661 ctttcgacgc cgcgtaaggg ctgttcggcg cataggccgt ctcttcagta aacgctggat
      721 catgcgggcc aagcgagccg tagacctcat cggttgaaac atgaagaaag cgaaattgct
      781 tctgctctac tgcacccagt tccatgaaat acgcccgcgc cgcctgcaac agggcaaatg
      841 ttccaaacac attggtctgg ataaaagcgt ccggccccac aatcgaacgg tctacatggc
      901 tttccgctgc aaaatggaca acggcgcgcg gtctgtgttc gcgcaggagc gaggccacca
      961 gcttttcatc cgctatgtct ccgtggacga aaatgtggcg gctgtcgccc tccagagtgc
     1021 gcaggttctc ccggttcccc gcgtaagtga gattgtcgag gttgataacg ggagagccaa
     1081 cgtgccgtat ccactccagc acaaaattgg agccgataaa ccccgcgccc ccggtaacca
     1141 gtatcggacc agaacgattc ttagccgtct gcatagtcaa tgactactgt acatcagggc
     1201 ctcgtccgga aggctacccc cgcctaccgg tagctccgct ggtaggtccg cgtgatgcgg
     1261 tcgtgccatc cgagatggtc ttcatcgaag aacgaccaca gaaaacccag ccccatcggc
     1321 aaggccgaga gcagtagcgc gcccacacgg ttccgcatca ttttgcgcgt cggattttca
     1381 tcgtcaaagg tgcagagggc tatcttcgca taccgcatac ccggcgtcgc atcggaatag
     1441 gtgaaaaaca gccattgata aagcacaaac aggacgccca gagcgatgct cgctccggca
     1501 atcgccggct ttcctgtcgg cggatgcgcc gtgcaggcca caaataccag cacaaagaca
     1561 aaaaacgatg ccaacaccag cgctgtatcc acaatcccgg ccatcaggcg gtcttccatg
     1621 gaggccacct tcagcggcag atcgaaaaga tggctgtagt ttgccgctgg agcggcggct
     1681 tctgcgggct gcatttcctc ggcaggcttc gcatccagcc gaatcgagtg ccactccggc
     1741 agcacaggct ccacgacggg gtcctttgaa atgtcctccg gttcaacttc aaagatgcgt
     1801 agttgggaac cttccggccc gggatctgct tcgcgcaaag ggccttcggc cagccgcgga
     1861 cgcgccttgc gcggagcaat cagctcgcga ggaaactcga tcagcttcgc cggcaaaggt
     1921 tgtggaggcg tcaccgtgac ttcttcgaag gggtctgccg cttccgccac catgacatgc
     1981 ggggtctcgg ccacaggccc gggctgcgca gctccggaat cctcttcttc gtgggcctcc
     2041 agtcccgcaa gcgctgcctg cgtggcggca tgggcctgca tggctgcctc ggctgctgct
     2101 tccgcggcgc gagcggccgc cgcctgcgcg gccagaagct cctgatacga cggggccttc
     2161 gagtagcgct cggccacccg ctcagccacg ctctttcgcc cgccttcggc atggcgcaga
     2221 tcaaatccgg gaagcgctgt ctggctttcc ggcacacgcg tgcgccgcgt gcggtgcgct
     2281 gccagacgcg cgtttacttc gtctttccaa ttggcggcag aatcaaattg caggctcgag
     2341 gtcgaagtgc tcaatggagt tccccgtaaa agttaacaac cctatatgtt cgaggagcgg
     2401 aggcagcaga gacacaccaa acccgatacc cagccatgcg cgtgctgaag cggcagacat
     2461 ccggtttgtg gagagccaga gcagcgtgct ttctccttct gaagggcaga cccgaagcct
     2521 gttatgctga aaattccaga tttgcccctc gtgatgagag cccgcatctt tttgtgtatc
     2581 acgctgttgt gcctctgtca tccgcagctt tggccgcagg cagtgacaaa cgagtttcct
     2641 ccgccctcgc agctcccgca gcttccggat gcgcccgatg ttcccgtggc ccgcgtcgtg
     2701 cccgctccgc caggcggcgt ccccgtcgaa atccgcgcca tctcccagcg cgagaaggac
     2761 aatgtctaca cactggaagg caatgtcgtc atacactatc gcgattacgt catacaggcc
     2821 gacaaaatcg tctataaccg cgatacgggc gaggtcgtcg ccgacggcca cctgaccctc
     2881 gatggcagcc cggacaatgt ccacctgacg gcgacccacg gccaaatgaa cgtagacgcg
     2941 gacacggcgc atttctatga cgtcatcggc accatcggtt ccggctccga acgcaccctg
     3001 cctcgcaata aaagggtctt cacctcaccc aatccttttg ccataacagg acgcgaactg
     3061 atcaagctcg gcccgggcag ctacaaggtg atagacggaa ccatgacctc atgccacttg
     3121 cccagcccgg actggcggct tctcgccaaa gaaatcagcg tggacaacgg cacagccacc
     3181 gcacgcaaca ctgtctttca actgctgcat gtcccgctgc tatacctgcc ctatgccacc
     3241 catgccgtca acgaggagca gcgcacctcc ggcatcctgt tgcccatcat cggcaacagc
     3301 acgaccaagg gattcatctt cggcgaacag gtctactttg cgctgggccg caactctgat
     3361 ctgaccctcg gtacggaata cttctccaaa cgcggctttt cccccatggg cctcttccgt
     3421 tatcgcggat tcggcgagga cttcttcaat gtgcgtttcc actcgctgct ggaccgtggg
     3481 ctccctccca accacattga ccagggcggc gtagacctga tgatagacgg ccgccgcgac
     3541 tggggcagcc atacacgcgg catcgtggac gcggaatatc tcagctccta catctaccgt
     3601 caggcctttg aagaaaacta tgccaccgcc attgactctg aagtgaagtc gcagctgttc
     3661 ggcgtgcatg acctcaatgg atattcgcaa agcctgcgcc tggaccggta ccagagcttc
     3721 cagaacacca gcaccaacgc ggaaatccgt ctcctgcaca tgcccgagct aagactcgaa
     3781 gccgaagacc attacctcgg caacgatacc tcgctcatgt ggggcggcat cgcgtccgta
     3841 gcggcgctgt cgcgttcaga gcctgccaac agcctgaata cggcgacctt ccgcgcccac
     3901 tttgtgccgc gcgttgatct gtatccgcac atcgcattgc ccttccatct cgatggctgg
     3961 agttttcgcc cagaagccgc tgtacgcgag acgttttacg gaaagagcca gacgccggca
     4021 cccctgggca ccgtgccatc tttgcgcaat gccagcgtag aacggaaaga tgtggaagca
     4081 ggcattgacg ttcgcccccc tgccattgaa cgcgacttca gcgccccctg gatcacccgc
     4141 ctctttggcg gtgacctgcg ccattccatc gagccggaca tccaatacca ttacgtcgca
     4201 ggaatctcaa agttcgactc catcctgcgc tttgatgatg tagacatcgc cagtgacacc
     4261 agcgaactcg actactctct tacccagcgc ctcttccgga gacatctgca cccgcatccc
     4321 tgcaaaggag acgaggccct cgggccagac gacctctgcg gaggggggtc ggtggactgg
     4381 ctctcatggc agctcgcgca aaaatatttc tttaacaata catttggcgg cgccgtcact
     4441 accggcacgc gcaacgtgct cgccaccacg ctggacctga ccggaatcgc cttcctcacc
     4501 gggccgcgca ccttctcgcc catcacctca cggttgcgga tgcgcacctc atcagccacc
     4561 gaccttgagt gggacctgga ctacgacccc cgcctgggcc gcatcacggc aagcaacgtg
     4621 tacgccggat ataaattcgg tgattacaac ttcaatctcg gcgagtttca tctcaacgcg
     4681 ccggaggccc cggccaccgt cggccagggc gcacagacca caagcatcat ttccaattac
     4741 gagcagcttc gcctcaccgc cagctatggc tcttctacca aggccggact gagcgccggc
     4801 ggcaccctgg gttacgactt tgtccagaac cagttgcagt acggcgccat ccaaagcgcc
     4861 tggaactggg actgctgcgg cctgagcctt gaagtccgcc gctactcgct gggtgcggtg
     4921 cgcgatgaca cccagtatct ctacagtttt acgctcgccg gagtcggctc tgccggaagc
     4981 ctccgtcgcg ccgtgcgcat cttctgattt atggacatta aggcacactg gcagggcatc
     5041 tacggcacaa agtcgcctga cagtgtcagt tggtatgcac cacatcttga gatgtccctg
     5101 cggttgataa atcacgcgaa tctcagccgt gatgcgtaca tcctcgatgt aggtggaggc
     5161 gcgtccacac tggccgatga tctattgacg cagggataca agcatatcgc ggtacttgat
     5221 atctcagaag ctgcactgaa tatctctcgg aaaaggctgg gcgaacaagc gggccgtatc
     5281 cggtggatcg caggggacgt gacctcaact caattgcagg agcatgccta tgacctatgg
     5341 catgaccgcg ccgtcttcca tttcctgaca acaccggagc agcgcgccgc ctacattcgc
     5401 caattgcttc acacactgaa gccaggcgga cacctcgttc ttgctacatt tgcttcttgt
     5461 ggccctgaaa gatgcagcgg cctggaggtc gtccgctata gcactgactc gctcgcgcga
     5521 gaactcggcg aaaattttta cctcatggaa agcgcagagg aagaccatat caccccttcc
     5581 ggtgcgaaac agccttttct ttattgccgc tttcagatcg tctaacgaat gagataatca
     5641 aggtgtagat ttctcccctc tgggaaaggt ggttttcttg cttcggaccc gcttcttttt
     5701 tggctgcctc gcattgctgc tggcggctgg ctgcaaagcg caggacaatc ccaacgacct
     5761 caataacgcg aaaatagacc gccgtatcga ggtcctggtg cgctcgcagc tcaatgttcc
     5821 tcaggattgg aaagtgaaca tcggcccgcg ctcgaaaagc gatgttccgg gatttgacgc
     5881 tgtgccgatc acgttctctt ccatgagcga ctcttcaaag cagcagaccc tgaattttct
     5941 gctctcaaag gacggtaaca ccctcgcgcg cctctccaaa tgggacttga gcaggaaccc
     6001 agccgacctg atttcgacag ccaatcgccc cgtccgcgga aaccctgatg ccaaagtcac
     6061 catcatcaac ttcgatgatc tcgaatgccc ttactgcgcg cgcatgcacg ctcagctttt
     6121 tccggacacg ctggaccgct acaagggact gatcaaagtg gtctataaag actttccgct
     6181 ggttgaaatc cacccgtggg cgctccacgc cgctgtagat gccaactgcc tcgccgccca
     6241 aagccccacc gcctactgga actatgtgga ctacctccac acccacggcg atgacgtaag
     6301 cggaccgcag cgcgacccgg cgaagtctgc cgctacgctc gataaacttg cccgcgacga
     6361 aggtcagcgc agcaagctcg attccggcaa gctcgatgca tgtctggcga aacaggacga
     6421 ttctgtcgtg cgcgcttcca tgaaggaagg cgacgctctc ggagtggatg gcactcctac
     6481 actcttcatc aacggtgaac ggctctccgg agcgctgccg gaagagcagg tatggatggc
     6541 gattgaccgt gccctgaagg ccgaaggcat tacccctccg gccccggaaa aacccaccgt
     6601 ccctgccgcc ggaaaaggcc tgggcaaata agcaatgcac ggccttcggg tggtacattc
     6661 agagataatt gtggataaat gggagattgc gccggttgcg cctccaggga gataagtgtt
     6721 gctacgtgta ctgactgttc ctcgtaattc ttcagctttc cttccccgct ttcagcagat
     6781 ggcgatggtc tgtgttgcct ctctggccat ggccggagct gccgggtgcc atcgcacaca
     6841 cggaccggac gtggtcgcca ctgtaaacgg caagcccatt atgcgctctg aagtagagca
     6901 gctctaccag aacaatctcg gcgattccca gcagcagccc tcaaaggaac aggctgatat
     6961 cgtgcggctg aacatcattc gccagttgat tgatgaagag atcctgatgc agcgcgcggc
     7021 caagctcaat ctcaccgcca ccgatgagga agtggacaat cagctcaacg aactgaaagc
     7081 gccgtttacg caggaagaat ttaacaaacg cctggccgac aagcatctga ccctcgatga
     7141 cctgaagcgc cagatccgcc gctcgaagac ggaagacaaa ctgctcaata aagagatcaa
     7201 ttccaagatc aacattacag atgcggacat ctccagctac tacaacgcgc acaaatcaga
     7261 attcaacctg attgagccga tgtatcatct ggcgcagatc attgtcactt cgcagccgct
     7321 gccgcagcag cagcagacat caaaccttca gaacagcaaa gccacgaacg acgcagaggc
     7381 gaagaagaaa atcgagatgc tgcacaaccg ccttgaaagc ggcgaggact tcggtcttct
     7441 ggcccagaac ttctccgagc gtccggacac tgcctccaat ggcggcgata tgggctttgt
//
LOCUS       DASRBV010000173         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_263674, whole genome shotgun sequence.
ACCESSION   DASRBV010000173 DASRBV010000000
VERSION     DASRBV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263674"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFA02_06520"
     CDS             complement(<1..725)
                     /locus_tag="VFA02_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU09542.1"
                     /translation="MLNGTDQEEVGVQTTPEQPVRVLLLDDQEDNLLLRSTILRKYGY
                     VTQTASTIEEAEKLLDEIDIAVLDYHLGAGKFGTEVAASLRRRHPEVPIIILSATLER
                     RFGGPEDMHLLKGYSSIDDLVAALRSFEAKKRGKPVVVDAREFFYSRINMAMGDDIVL
                     EVLDADGVWQYVNDTCAKLLEHPREWFPGRNMFEEMPDLAPDWRDILHTVAETRETYL
                     DRTYRGLLNLPRKGEEWVWNVLA"
     gene            complement(843..1847)
                     /gene="lpxD"
                     /locus_tag="VFA02_06525"
     CDS             complement(843..1847)
                     /gene="lpxD"
                     /locus_tag="VFA02_06525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZU09543.1"
                     /translation="MNLGELAKRLGAECRGNPETKITGVAAIESAGPGEVTFVANPRY
                     AALAKTTRASAVLVAPDFPEIKAATLRISNPYLAWARAIEIFHPAPTYPLGIHPTAVI
                     APTAQIGEGASIGAYAVIGEHCVIGKRAVILPHAVIYPNVRIGDDFFAHAHAVVREGC
                     VLGHRVVLQNGVVVGADGFGFAKDNEGRWRKIVQTGPVIIGDDVEIQTNSCIDRASVG
                     ETRIARGAKIDNLVQVGHSSTVGEDTLLCAQVGLAGSTHIGKSAILAGQVGVAGHCHI
                     GDKVIVTAQSGIGGDVEAGKIVSGSPAYDNKQWLRVSALMPRLPEILKRLERAIKSKE
                     "
     gene            complement(1894..3132)
                     /locus_tag="VFA02_06530"
     CDS             complement(1894..3132)
                     /locus_tag="VFA02_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09544.1"
                     /translation="MTFTSRLRFRAIALLAFLAPLGALAATVTGTVTNKTNNRPAAGD
                     DVVLIAFGQGMQEAARTKTDAHGHYSIDVPDNGMHLIRVDHQKAAYFQPLRPGMTTAD
                     VDVYDVQPKVEGVTTEADVIRVETDQQGLHVVENYFVKNDSDPPKTQFGPRAYEIYLP
                     PDARIEGSAAMGPGGMPVSSSPVPTGEKGYYAFVFPVRPGETRFQVSYHLPYNGSFTF
                     TPKVSLPTQNVAVMLPESMKFAAGGTVPFQAINEDVNAQTFLARNVSPSQAMAFTVSG
                     SGSMPREEQGQGGDQSGQASAANDNRPGGGLGNPIDTPDPLNKYKWWILSGLALILAI
                     AAAFLLRGKPETPASSAASPTPAPAGQRDLLSALKEELFVLETERLEGKLSESEYSEQ
                     KAALETVLRRALSRQTILQP"
     gene            complement(3225..3527)
                     /locus_tag="VFA02_06535"
     CDS             complement(3225..3527)
                     /locus_tag="VFA02_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09545.1"
                     /translation="MSTIACALLLVGVFAYVFWPERRVASQTQKTRLDFLRERKDVLY
                     DNLRDLNFEYRAGKYPEEDYAAQRAALENEAAEVVAEIELLEKQASGLPSFSKQDT"
     gene            complement(3595..4050)
                     /locus_tag="VFA02_06540"
     CDS             complement(3595..4050)
                     /locus_tag="VFA02_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZU09546.1"
                     /translation="MRRISQIGVLALLLVLTLGSADTAVRYTELNHKMMCACGCGQVL
                     GECNHLGCPDSERMLTELRTAISKGDSDTAILEAFQAKYGPTILAAPMLTRFNIVAWI
                     VPPVVLLIGIFGTFALVRKWKLRTVPTPGPSSVRGFDRIRDQIRKETEL"
     gene            complement(4050..>5915)
                     /locus_tag="VFA02_06545"
     CDS             complement(4050..>5915)
                     /locus_tag="VFA02_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZU09547.1"
                     /translation="ALVWAAFSNDFSVAYILHHSNRALPGPYKFAALWSGQEGSLLLW
                     AWLLTGYGFVLRLRHKVDVKLTAYASTILAGIQVFFLLLLNFAAPPFSLVSGTVPQDG
                     FGLNPLLQYPEMVIHPPMLYLGYVGFSVPFAFALGALMMRYPGEKWIHITRRWTMVTW
                     LFLTCGIFLGAHWAYSVLGWGGYWGWDPVENASLMPWLTGTAFLHSVMMQEKRGMMKH
                     WNVWLIFSTFALSILGTLLTRSGLVSSVHAFAQSSIGSWFWAFLVIVLAVCLFTYILQ
                     RDHLKSEHKLESLVSRESSFLFNNLVLLAACFTILWGTLFPVLSEYVQGNKVTVGPPF
                     YNRVAVPIGLFLLFLTGIGPLLAWRSSSFKSIRKNFILPAVAAFVTAIVLVAFGMRPW
                     QDSGAFYSLVAWSLAAMVAVAISSEFARGARVIQRHTGQGLFASMLQLTRRNTRRYGG
                     YIVHFGVVVIFIGLAGSAFNQSKEQELNYKQSMTMGPYRLECLDYSQDSNPNYDTEYA
                     LLDVYRGGKKITQLAPEKRFYQASQTTSTIVANHSTLAWDLYVIYAGKDPDNGQPIIK
                     VFLNPLVAWIWIGVIIVVLGTFVALTPNMAAALAASRSRVPVAEAPVLAATKGGD"
BASE COUNT         1270 a         1626 c         1802 g         1217 t
ORIGIN      
        1 aaggccagca cgttccagac ccactcttcg cctttgcgcg gaaggttcaa cagcccgcga
       61 taggtgcggt caagataggt ttcgcgtgtc tctgcgaccg tatgcagaat atcgcgccag
      121 tccggggcga ggtccggcat ctcttcaaac atattgcgcc ctgggaacca ttcgcgcgga
      181 tgctcaagca gcttcgcgca ggtgtcgttc acatattgcc agacgccgtc ggcgtccagc
      241 acttcgagca cgatatcatc gcccatggcc atgttaatgc gcgagtagaa gaactcgcgg
      301 gcatctacga cgacgggctt gccgcgtttt ttggcttcaa aggagcggag agcggccacc
      361 agatcatcaa tcgaactgta acccttgaga aggtgcatgt cttcaggacc gccgaagcgc
      421 ctttcgagcg tggccgaaag aataatgata ggtacttcag gatggcgccg gcgcaggctg
      481 gcggccacct ctgtgccgaa cttgcctgcg ccgaggtgat aatcaagcac agcgatatca
      541 atctcatcca gtagcttctc ggcttcttcg atcgtggatg cggtctgcgt aacatatccg
      601 tacttgcgca ggattgtgga ccgcagcaga agattgtcct cctggtcatc cagcaaaagg
      661 acgcgcacgg gctgttcagg cgttgtctgc acgccaactt cttcctgatc ggttccgttc
      721 agcattgatt ggtgggatgc ggaagcgggc cgtaaagctg ctattcttca tgattttgca
      781 ttgtacttac cctgcacaat cgtgccggaa aggagcgata tgcggacgcc gcgagtccaa
      841 ggctattcct tgcttttgat ggccctctcg aggcgtttca gtatctccgg cagacggggc
      901 atcagggcgg aaacccgcag ccattgtttg ttgtcataag ccggagagcc gctgacaatc
      961 tttcccgctt ccacatcgcc gccaatcccg gactgcgccg tgacgatgac tttgtctccg
     1021 atatgacagt gcccagcgac gcccacctgt ccggcgagaa tggcgctttt cccaatatgc
     1081 gtagagccag cgagtccgac ctgggcgcag aggagcgtgt cttcaccaac ggtggaactg
     1141 tggcccacct ggacaaggtt gtctattttg gcgccgcggg caatgcgcgt ttcgccgacg
     1201 ctggcgcgat caatgcagga gttggtctga atttctacat cgtccccgat gatgacggga
     1261 ccggtctgga cgattttacg ccagcgtcct tcattgtctt tcgcaaaacc gaagccatct
     1321 gcgccgacga cgacgccatt ctgcagcacc acgcgatgac cgagaacgca gccttcccgc
     1381 acgacggcgt gagcgtgggc aaagaagtca tcgccgatgc gcacgttcgg gtatatgacc
     1441 gcatgcggaa ggatgacggc acgttttccg atcacgcagt gttcgccgat gacagcatat
     1501 gcgccaatgg aagctccttc gccaatctga gcggtgggcg caatcactgc ggtcgggtgg
     1561 atcccgagag ggtatgtggg ggcggggtgg aagatttcga tggcgcgcgc ccaggccaga
     1621 tacggattcg agatgcggag ggttgcggct ttgatttccg gaaagtccgg cgcgacgagg
     1681 acggcgctgg ccctggtggt cttcgccaga gcagcgtagc gcgggttggc gacaaaggtc
     1741 acttctcccg ggcccgcgct ttcaatggca gcaacaccgg taatctttgt ttctggattg
     1801 cctcggcact ccgcgcccag acgctttgcc agctcgccta agttcatgga gaaattctag
     1861 cccaccctta acctttcaac tttgagcaga gttttagggt tgcaggattg tctggcgtga
     1921 aagggcgcgg cgcaagacgg tctcgagcgc tgccttctgc tcgctgtatt ctgactcgct
     1981 cagtttgcct tccagccgct cggtttccag aacaaaaagc tcttccttga gtgccgagag
     2041 aaggtctctt tgccccgccg gagcaggtgt gggcgaggca gccgaagatg ccggcgtttc
     2101 cggtttgccg cgcagcagga aggccgcggc aatcgccagt atcagagcga gaccgctgag
     2161 aatccaccat ttgtatttgt tgagtggatc gggtgtgtct atgggatttc cgagtccgcc
     2221 gccggggcga ttgtcattgg ccgcggatgc ctgcccgctt tggtcgccgc cctggccctg
     2281 ttcctcacgc ggcatggaac cgctcccgga gacggtaaaa gccatggcct gcgaaggaga
     2341 gacgttgcgt gcgaggaacg tctgcgcatt cacatcttca ttgatggcct gaaaggggac
     2401 cgtgccgccg gccgcgaact tcatgctttc aggcagcatg acagcgacgt tttgcgtggg
     2461 cagagagacc ttcggagtaa aagtgaagct gccgttataa ggtaagtgat agctgacctg
     2521 gaagcgtgtt tctccgggac gcacagggaa aacgaaagcg tagtagcctt tttctccggt
     2581 cggaacaggc gaggaagaaa caggcattcc tcccggcccc atggcagcgg agccctcgat
     2641 gcgagcgtcg ggaggcaggt aaatctcgta ggcgcgggga ccgaattgcg tcttcggtgg
     2701 gtcggaatcg tttttgacga aataattttc aaccacgtgg aggccctgct ggtcggtctc
     2761 aacacgaatg acgtcagcct cggttgtaac accctccact tttggctgta cgtcgtagac
     2821 gtccacatcg gcggtcgtca tccccgggcg caggggctgg aaataggcgg ccttctggtg
     2881 gtccacgcgg atgaggtgca tgccgttgtc gggcacgtca atcgaatagt ggccgtgggc
     2941 gtcggttttg gtgcgagcag cctcctgcat cccctgtcca aaggcgatga ggacgacatc
     3001 gtctccggca gcgggcctgt tgttcgtctt gttcgtaacc gttccggtta cagtagcggc
     3061 aagcgcgccc agaggagcaa gaaaggccag cagcgcaatc gcgcggaatc gtaaacggga
     3121 agtgaatgtc atgaatatct ttcttcttat cacaatgctg tcatcctgaa gaccctgcgc
     3181 ttgcgaaggg gacgaaggag gtgtttttta ttccgggtgc cacatcaggt gtcctgtttt
     3241 gagaaagatg gtaacccaga cgcctgcttt tccagcaatt caatctctgc cacgacttcg
     3301 gcggcctcgt tttccagggc cgcacgctgc gcggcatagt cttcttccgg atacttcccg
     3361 gcgcggtact cgaaattcag gtcacgcagg ttgtcataga gtacgtcttt gcgctcacgc
     3421 aggaagtcga ggcgcgtttt ctgggtttgc gacgcgacgc ggcgttccgg ccagaagaca
     3481 taggcgaaga cgccaaccaa aagcagtgcg caggcgattg tactcatcgt ccactctcgc
     3541 attcgtaatc tttgccggga gggcctgtcc tgggcttgtc gaagggaagg gtcctcatag
     3601 ttctgtctct ttccggatct ggtcgcggat gcgatcaaac ccacgaacgg atgaaggacc
     3661 aggtgttggg acggtccgca gcttccattt gcgtaccagc gcgaaagtgc caaagatgcc
     3721 aatcagcaac acgaccggag gcacgatcca cgcgacgatg ttgaatctgg taagcatggg
     3781 ggcggcgaga atcgtcggcc catactttgc ctgaaatgct tcgaggatgg cagtatcgct
     3841 gtcgcccttg ctgatggcgg tgcgcagttc ggtgagcatt cgttccgaat cagggcagcc
     3901 aagatggttg cattcgccca ggacctgtcc gcagccgcag gcgcacatca tcttatgatt
     3961 cagttcggtg taacgcaccg cggtatctgc cgagccgagg gtaaggacga gcagcagcgc
     4021 gaggacccca atttgtgaga ttcgccgcat cagtcgccac cctttgttgc tgcaagaaca
     4081 ggcgcttcag cgacaggcac tctgctgcgg ctggcggcga gggccgctgc catatttgga
     4141 gtgagcgcga cgaaggtacc cagcaccacg atgatgacgc caatccatat ccaggccacc
     4201 agcggattga ggaagacctt aatgatgggc tgtccattgt ccgggtcttt ccctgcatag
     4261 atgacgtaga ggtcccacgc aagtgtggag tggttggcga cgatggttga ggtggtctgg
     4321 ctggcctgat agaagcgctt ttccggggca agctgcgtga tctttttgcc gccgcggtag
     4381 acgtcaagca gggcgtactc ggtgtcgtag ttcgggttcg agtcctgcga atagtcgagg
     4441 cactcaagcc ggtacgggcc catcgtcata ctttgcttgt agttcagttc ctgctctttg
     4501 ctctggttga aagctgaacc ggcgaggccg atgaagatga ccactacgcc gaagtggacg
     4561 atgtatccgc cgtaacggcg tgtgttgcgg cgcgtgagct gaagcatgga agcgaaaagg
     4621 ccctgtccgg tgtgtcgctg gatgacccgt gcgccgcgcg caaattcaga ggagatggcg
     4681 acagcgacca tcgccgccag cgaccatgca acgagcgaat agaatgcgcc ggaatcctgc
     4741 cagggacgca ttccgaaagc cacaagaaca atagcagtta caaatgctgc cacggcgggc
     4801 aggatgaaat tcttccgaat gctcttgaat gatgaactgc gccatgccag cagcggcccg
     4861 attccggtaa ggaacagcag aaacagccca atcgggacag ccacacggtt ataaaacggc
     4921 ggcccgacag tgactttgtt cccctgaaca tattcagaga ggacagggaa gagcgtcccc
     4981 cacagaatgg tgaagcaggc agccagcagg accaggttat taaagagaaa gctggactca
     5041 cgcgaaacca gcgattccag cttgtgctcg cttttgagat ggtcgcgttg caggatataa
     5101 gtgaacaggc agacggcgag cacgatgacg aggaaggccc agaaccatga cccaattgac
     5161 gactgcgcaa aggcatggac ggaactgacc agtccggagc gtgtgagcag agtgccaaga
     5221 atcgaaagcg cgaatgttga aaagatgagc cacacattcc agtgcttcat catgccgcgc
     5281 ttttcctgca tcatgaccga gtgcagaaag gcggtgccgg taagccaggg catcagcgag
     5341 gcgttctcga cggggtccca tccccagtag cctccccatc caaggacgga ataggcccag
     5401 tgcgcgccta gaaagatgcc gcaggtcaaa aagagccagg tcaccatggt ccaccggcgt
     5461 gtgatgtgga tccatttttc gccggggtag cgcatcatca gcgcacccag ggcaaaggcg
     5521 aacggcacgc tgaatccgac atatcccaga tagagcatcg gtggatgaat caccatctct
     5581 ggatattgca gcaacggatt gagcccgaat ccgtcctgag ggaccgtgcc gctgacgagc
     5641 gaaaacggcg gcgcggcaaa attcaacagc agaagaaaga agacctgaat gccggcaagg
     5701 atggtcgagg catatgcggt cagctttaca tctactttgt gccgcagccg cagtacaaat
     5761 ccgtagcccg tgagcagcca tgcccataaa agcagcgagc cttcctggcc cgaccagagg
     5821 gctgcaaatt tataagggcc ggggagggcg cggttcgagt ggtgcaggat gtaggcaacc
     5881 gaaaaatcat tgctgaaggc cgcccacacc agggc
//
LOCUS       DASRBV010000174        17859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_674724, whole genome shotgun sequence.
ACCESSION   DASRBV010000174 DASRBV010000000
VERSION     DASRBV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 17859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17859
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674724"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFA02_06550"
     CDS             <1..1062
                     /locus_tag="VFA02_06550"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZU09548.1"
                     /translation="TRREMLAAIARHHCAPVVMVNQVGGMDSLIFDGSSLVLSRDGEV
                     IAQGKSFEEDLIFADLETGRGDVHYQTDNIDEAVYSALVLGTRDYVRKCGFSKAIIGL
                     SGGIDSALVATIATDALGKENVLGVGMPSPYSSQGSIDDSRHLAENLGIRFEMVRIDD
                     LFAEYNKALAPLFAGCRPDITEENIQSRTRGVLLMALSNKFNALVLTTGNKSEMATGY
                     CTLYGDMVGALAVIGDLMKTRVYDLCRFANSRSERIPQAILDKPPSAELRPGQKDTDS
                     LPPYEVLDPILEAYVENYETPEEIAAKQRVDIELVRRVVKLVEHSEYKRQQAAPVLKV
                     TKKAFGPGRRFPIACKVQV"
     gene            1179..2150
                     /locus_tag="VFA02_06555"
     CDS             1179..2150
                     /locus_tag="VFA02_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU09549.1"
                     /translation="MFAMIIAACVPAFAQQNQPAEHSAPTASTEEVPTAPSAPAPTAG
                     TPNFPPADPANFTAATPSKETVNAFLQQLWGYDPDRVWQVQAIQKTAAPGVSKVTVLA
                     AQKTNPQQIGSLQFFTTPDGQHLISNEVMPFGARPFDATRKLLQTGANGPSRGAAGKE
                     LLLVEFADFQCPHCKEAQPTVERLLSDFPNAHFVFENLPLTSIHPEAYKAATFGVCVA
                     KLGGNDAFFKYADTVFTNQASLTPEAAATTLNDAATKAGVDAAKASACSDSAEAKSTV
                     DASLHLAQDLSVNETPWLFINGRGLPLNSVPYETLKKIVEFQAQNDK"
     gene            2325..4691
                     /locus_tag="VFA02_06560"
     CDS             2325..4691
                     /locus_tag="VFA02_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HZU09550.1"
                     /translation="MEQGRWVLLIASEAGGTDSVADQAMERLVQAIQEEGYRVVRTST
                     PEDGLALVTSDPSYSAILLDWDLPENNQFDERAALRIIRAVRRRNKKLPIFLIADRTL
                     VSELPLEVIKQVHEYIHLFGDTPAFIANRVDFAVERYHEQLLPPYFRELVKYTDQGAY
                     SWDAPGHMGGVAFLKHPVGMAFHKFFGENLLRSDLGISTSQLGSWLDHIGPPAESERN
                     AARIFGADWTFYVLGGSSTSNQIVGHGVIAQDEMVLADLNCHKSICHSLTVTGARPVY
                     FKPTRNGFGMIGLVPLARFRPENIAEQINKSPFVAGAVSKTPSYAVVTNSTYDGFCYD
                     VNAVVEELKKSVPRVHFDEAWYAYAKFHPLYRGRFAMDVPDEMPDRPLLFAVQSTHKM
                     LPAFSMASMIHVKLSPRAPLEFDQFNESFMMRGTTSPFYPMIASIDVATAMMDEPAGS
                     TLMMETIEDAISFRKAMASVAHRLKAEEGGGWFFGMFQPDTVTDPDTGEVYRFEDAPD
                     DLLSESPKCWTLKPGDAWHGFRDEDIANDYVLLDPTKVTILTPGIDAHGNMGEWGIPA
                     AILTAFLDARRVEIARTGDYTILTLFSVGTSKGKWGSLLETLFEFRRLYDTEAPVNDA
                     LPQLVAKYPERYGMMDLKQLSDEMHAAMKELNLPGLLQEAFDVDLPQVMTPTQAYQRL
                     IREGTEKIRLTEMAGRISAVMLVPYPPGIPMCMPGERLGDANSPTIRFLLALEEFGKR
                     FPGFEREVHGIEVDSEGNFWMRAVIETPGKKTNGRSKPRTHPSSRVAK"
     gene            complement(4716..5762)
                     /locus_tag="VFA02_06565"
     CDS             complement(4716..5762)
                     /locus_tag="VFA02_06565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU09551.1"
                     /translation="MPDASRLARTPLSILDLAPIKEGHSISGSFRETLDLAQHAEQWG
                     YKRYWLAEHHNLASVASAATAVLIGYVAGGTSTIRVGAGGIMLPNHAPLVIAEQFGTL
                     ESLYPGRIDLGLGRAPGTDYPTMRALRRELHNGDNFPQLVAELRGYFAEPKPGQAVKA
                     IPGAGLNVPVWLLGSSGFSAQLAGDLGLPFAFAAHFSPADLFSALEIYRHYFRPSEVL
                     EEPYVMVGVPVLAADTNEEAQRLSSTEYITVLRLIRSQLAPLSPPVDSVEGLANDLEH
                     AAITSKRAAAIYGDRNTVRTKLEAFLEKTRADELILTCNPYEHAARLRSYQIVAEIQR
                     EAQAELEAMPVSSR"
     gene            5963..6039
                     /locus_tag="VFA02_06570"
     tRNA            5963..6039
                     /locus_tag="VFA02_06570"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5997..5999,aa:Glu,seq:ctc)
     gene            complement(6170..7201)
                     /locus_tag="VFA02_06575"
     CDS             complement(6170..7201)
                     /locus_tag="VFA02_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZU09552.1"
                     /translation="MSERDGRILWVDVCKGLGIICVVLGHSIDGTALQRFIYTFHMPL
                     FFFLSGFLHRPQPDYGLFAKRKAIHLLLPYVTFMLLLCPLAYISAFHHPEKWGALTKT
                     FLWGGGQMEGRYGVLWFFTCLYLTQQIANYLLVRFRPGMVLVAGLVSLTGAYLLTDLA
                     PHFTLPLNAHVVLGALPLLLAGYLYRKSSHQRLLELLAIPGLILTVWLVRLHVPISYD
                     MKSGNYGVPGLSLVLGLSCILVVIALSRIITRTAALTMVFASVGASSMGIMVIHKVVL
                     SMGLVSRLTKNGWAAFLVALISSWVVTLLLARFPVTRAFLLGSERDFQRLWTPSSVQP
                     KPVTEADAA"
     gene            complement(7422..8639)
                     /locus_tag="VFA02_06580"
     CDS             complement(7422..8639)
                     /locus_tag="VFA02_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU09553.1"
                     /translation="MQTEEQNRIDQPEEEQRQKPRRRFIIIGVVAIAVVIALLWWWHS
                     TYYEDTDDAQVSGHLIQISARVAGHVIKVNVDENQYVEAGTVIAELDPKDFETAVQQD
                     EANLAAAEAAYESANVNVPVTHINTRSNLSSAGADVSSAEAQVAQSQQQLQAAEAGVA
                     QADANYKKAQADLERYTPLVQRDVISKQQYDAAVATADADKAALDQAKANLAAAQAAV
                     RVAKDRVASAQASYSYAQQGPKLVAIQKARADQAAAQVQQARAALEQAKLNLGYTRII
                     APVAGIITKKSVEVGQNVSVGQNMATLVSLDDVWITANFKETQLAHMRPGQPVTISVD
                     AYGGRKYDGKVTQIGGATGSVLSLFPPENATGNYVKVVQRIPVRIDLTDHNQNSDHLL
                     RPGMSVIPKVKVK"
     gene            complement(8659..10116)
                     /locus_tag="VFA02_06585"
     CDS             complement(8659..10116)
                     /locus_tag="VFA02_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09554.1"
                     /translation="MRVIQNNRRWSAALGLFLLPWSAQAVLAQAQTGSSSSPLPSPYQ
                     IPAAQQGGSGFQGSVVQQKPTEGILPLSLDDAIQRGLKYNLGLVLSNENTLSANGSRL
                     QELQALLPVASASLKESVQQVNLAAEGLRIPGFPQIIGPFGYTDVRASLNWSLLNVAS
                     LQNYLAAKHNFQSAKLSAQDARDMVTLTVGNAYLLVIADKARIDSAQAQVNTSKVSLD
                     QAVQNHRAGTAPLLDELRARVDYQTQEQNLITAQNQYEKDKIALARVIGLPLEQKFEL
                     TDTAPYAALDEIDPETAVKQALANRNDLKAMQEQLRAAEKARSAATAERYPTISFAGD
                     YGDIGPTLSHSHGTGNATGTLSVPVLEEGKLRGDARQAQAQLESKRAQLSDLQGQISA
                     DVRDSILDIKAAQKQVEVARSNVQLANEALSEAQQRYAAGVSDNLAVSQAQQAVAQAD
                     DQYVSSLYQHNIAKLSLARALGMAEKNYKSYVGGK"
     gene            complement(10232..10807)
                     /locus_tag="VFA02_06590"
     CDS             complement(10232..10807)
                     /locus_tag="VFA02_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09555.1"
                     /translation="MGDQGCSELLAQDTAAKFFQTAALLVSNEADAVTLVEEAIAAAE
                     VDPCADGEAANALVQNKIVRAAIRLLSQREPEAFAASESAGQGSAGCIESDDLSTSGL
                     SHEQLTWLLEGEGRGRLRNWLEHLPAIQRTIFVERAMLGQNNAATAEDLRSSGGSGAA
                     GWTADKVSDVFRQALCSLATSLVQAGTAQPA"
     gene            10953..13127
                     /gene="ppk1"
                     /locus_tag="VFA02_06595"
     CDS             10953..13127
                     /gene="ppk1"
                     /locus_tag="VFA02_06595"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZU09556.1"
                     /translation="MSTRSRQQQSLLIGRDESWMQFNRRVLEEAQDVSNPLLERVKFL
                     AITASNLDEFVEIRVAGLLQRIEDGYTEAGPDGMTPQQTLDALNEDMQSFVVEQYKCW
                     NQQLMPALRQAGVRVLGWHEMDETAQESATAFYQREVDPLLTPVTIDPAHPFPRVLNK
                     ALCIALLLRRKRKNTAGPVLGVVTIPRALPRLVPLPSNQGTHDYIFLHDLIERHAAGM
                     YRGYEILSKAAFRVTRNSNLYFQEEEARSLLETVRSELHNRRKGDAVRLEIEQSADPE
                     IIDRLRMNFELDESQVYRTDGPVNLSRLMNLYSDTPRPDLKFQPFVPHELRLNRKSAD
                     LFDELRHRDILLHHPYDSFDAVVSFMELGAADPNVISIKQTLYRTSSDSAIFSALIEA
                     AQTKEVTAVVELTARFDEASNIRWAREMEDAGVQVFHGIVGLKTHCKLALLVRRDPDG
                     AVRRYAHLGTGNYNPNTARFYTDISLLTADPAITAGVHSVFNYLTAHSESDDYAPLLV
                     APLTLAESMIRLIARETEHARAGRPARIIAKMNSLLEQRVIEALYAASSAGVQIDLIV
                     RGICSLRPGLKEISENIRVRSIVGRFLEHSRIFYFANGGEEEIYCGSADWMPRNLFER
                     CEVVFPIKDAQLRARLRNEILAAYLADTIKTRMLLGDGKYFRLRETTLGKSLPAFSAQ
                     EFFMKVAEGKTTADDIPKMPEEQNPKPAVKKRRKVMTSSEMG"
     gene            13398..13670
                     /locus_tag="VFA02_06600"
     CDS             13398..13670
                     /locus_tag="VFA02_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09557.1"
                     /translation="MAFTETFHCDVCGKEKNEESEDWWLAWAERVSSVPDGANIPQLR
                     FVPWDVLLSHDARARHLCGARCAQTLMDRWMTSRGESTELQIPRET"
     gene            complement(13762..15129)
                     /gene="zwf"
                     /locus_tag="VFA02_06605"
     CDS             complement(13762..15129)
                     /gene="zwf"
                     /locus_tag="VFA02_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZU09558.1"
                     /translation="MDQTQSDALVFFGATGDLAYKQIFPALQRLAKRGRLQGPVIGVA
                     KAGWTLDQLKARAKDSVETHGGADAEGLPRLLEKLRYVDGDYNDNATFQQVRKELGDA
                     KCPLHYLAIPPILFGRVIDQLRDSGCANGARVVVEKPFGHDLESARALTAEIHRVFGE
                     QNIFRIDHYLGKNSVQNVIFFRFANLFLEPVWNRQYVESVQITMAESFGVQGRGAFYD
                     GVGAIRDVVQNHLMQVVSNIAMEPPPNPDVEALRDERVKVLKSVQTMDKDNLVLGQFE
                     GYLNEPGVKPGSTVETFAAMRLHINSWRWRDVPFFIRTGKSLPVTATEVIARLRQTPP
                     VFSEDLLPQNYVRFRLSPVPVIAIGGSVKEAGDRLRGCMVELEAEQECGKDNLLPYEE
                     LLDDAMRGNPAWFAREDYVEEAWRIFDNVLKDRPEVHRYKPGTWGPKEAARLTNDFGG
                     WSNPK"
     gene            complement(15273..15716)
                     /locus_tag="VFA02_06610"
     CDS             complement(15273..15716)
                     /locus_tag="VFA02_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZU09559.1"
                     /translation="MRREYPETPIIGVGAIILHEEKVLLIQRGKEPLKGEWSIPGGAL
                     ELGETLHDGLRREVCEETGLEVEPLQIVEVLDRIAHDEKGAVRFHYVLVDFLCRLTGG
                     ELHCSTDAVAAAWASREELNAHGKYRIAPFTLAVLEKAFQLAKKP"
     gene            complement(15821..16471)
                     /locus_tag="VFA02_06615"
     CDS             complement(15821..16471)
                     /locus_tag="VFA02_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU09560.1"
                     /translation="MANIMEKNLSQVIAQRRATPSFDGSPVPDEDLKKILNAGLHAPS
                     GYNMQPWRFIVVRSPEQKRRLRSASFNQAKVEEASAVIVACGDADGWRNGDLEEMLRL
                     GRDGGMPENYAEQAKVNIPNYLSNHPNFPMWLNRHVMIAFTTMMLMAEALGYDTAPME
                     GFEEEKVREVLKLPLSYHVVALLAIGHLKGPDKFYGGRFSMARTVFAEEYGKPIKL"
     gene            complement(16490..17476)
                     /locus_tag="VFA02_06620"
     CDS             complement(16490..17476)
                     /locus_tag="VFA02_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09561.1"
                     /translation="MNRWRWFLFPAVLLTGGIASAVTCTTQSQMTDPQRSAFVQAAHT
                     LATEIQSGNVSAIKANTISSVAAQFDPIASTIQSIAPDLQGATFTVQNIYALNASDLK
                     APADTQFFCSIPGSEVLITVSLAQLPPGNYAIVLVHSAGGKQPQQMALILANDPAGST
                     QWKLAGFYARPLTLAGHDSVWYWTQARDFAKKKQQWNAYFYYETAAYLALPADFLSSP
                     NLEKLQKEMNSTRPDGLPGSSPMTVAANGQTYEITNLHTDGSLGQLDLVVNYNATDTS
                     DPVATRSRNIDVMKALLAQHPELREGFHGLWVYANAPNQRPFGIEQPMNQIQ"
     gene            17565..>17859
                     /locus_tag="VFA02_06625"
     CDS             17565..>17859
                     /locus_tag="VFA02_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09562.1"
                     /translation="MRFSFSFWMMAAVVAGAQTAPPPHRVPGAKPVAAVSEQPAPVVP
                     AGPSSLLDQPAQPAHVQFDGHRLSVKADNSTLSGILNEISSKTGMKVDGPSGDQ"
BASE COUNT         3739 a         4651 c         5467 g         4002 t
ORIGIN      
        1 acgcgtcgcg agatgctggc ggcgattgcg cgccaccact gcgctccggt ggtgatggta
       61 aaccaggtgg gcggcatgga cagcctcatc tttgacggct caagcctggt gctttcgcgg
      121 gacggtgaag tgattgcgca gggcaagtcg tttgaagagg acctgatttt tgctgatctt
      181 gaaacgggga gaggcgatgt tcattatcaa acggacaata tagatgaggc cgtgtacagc
      241 gcgctggtgt tgggcacgcg cgattatgtg cgcaaatgtg gattctcgaa ggcgattatc
      301 ggactgagtg gcggcattga ttcggcgctg gtggccacga ttgctacaga tgcgctgggg
      361 aaggaaaacg tgcttggcgt tggaatgccg agtccgtatt cgtcgcaggg ctcgattgat
      421 gacagccgcc atctcgctga gaacctcggc atccggtttg agatggtccg gattgatgat
      481 ctattcgccg agtacaacaa ggcgcttgcg ccgctttttg ccggatgcag gccagacatc
      541 acggaagaaa acattcagtc acgcactcgc ggcgttttgc tgatggcact gtctaacaaa
      601 ttcaacgcgc tggtgttgac gacgggcaat aaatccgaaa tggccaccgg ctactgcacc
      661 ctgtatggag acatggtggg ggcgctggcg gtcatcggcg acctgatgaa aacgcgcgtc
      721 tacgacctgt gccgttttgc caacagcagg agcgagcgca ttccgcaggc gattctggac
      781 aagccgccat cagccgagct gcggccgggg cagaaggaca cggactcact cccgccgtat
      841 gaagtgctgg acccgattct ggaagcgtat gtggagaatt acgaaactcc cgaagagatt
      901 gcggcgaagc agcgggtgga tatcgaactg gtgcggcgcg tcgtcaaact ggtggagcac
      961 agtgagtaca agcgacagca ggctgctccg gtactgaagg tgacaaagaa ggcgtttggt
     1021 ccaggacggc gttttccgat tgcctgcaaa gtacaggtgt gacagagaag cctggggtga
     1081 tccacattct gaagggatgt ggatttgcag ggtttagact tttcttcagg aatccagaaa
     1141 ggacttcaat gcagcttttt acctcgactc gcaaagccat gtttgcgatg ataattgccg
     1201 cgtgtgttcc ggcattcgcg cagcagaacc agccagcgga gcactctgcg cctaccgctt
     1261 ctacggaaga ggtgccaaca gcgccgagcg cgcctgcgcc taccgcgggc actccgaatt
     1321 ttccgcctgc ggacccggcg aattttaccg ctgcaactcc cagtaaagag acggtgaatg
     1381 cgtttctcca gcagctttgg ggctacgacc cggaccgtgt gtggcaggtg caggcgatcc
     1441 agaagacggc cgctccgggc gtgagcaaag tgactgttct tgcggcgcag aagacaaacc
     1501 cgcagcagat tgggtcgctg caatttttta caacgccgga tggtcagcac ctgatttcga
     1561 atgaagtgat gccgtttgga gcgcgtccat ttgatgccac gcgcaagctg cttcagaccg
     1621 gcgccaatgg cccatcgcgg ggagctgcgg gcaaagagct tctgctggtc gagtttgctg
     1681 attttcagtg tccgcactgc aaggaagcgc agcctacggt ggaaaggctg ctgagcgatt
     1741 ttccgaatgc gcattttgtc tttgaaaatc tgccgctgac ctcgatccat ccggaggcat
     1801 acaaggcggc gacttttggc gtctgtgtgg cgaagcttgg cgggaatgat gcatttttca
     1861 aatatgcgga cacggtcttt acaaaccagg ccagcctgac tccggaggca gcagcgacga
     1921 ccctgaatga tgctgcgacg aaggcgggag tggacgcggc gaaggcttcc gcctgctctg
     1981 attcggccga ggccaaaagc acggtggatg cttcgctgca tctggcacag gacctgagcg
     2041 tgaacgagac gccgtggctc ttcatcaatg gccgcggact gccgctcaat tccgtacctt
     2101 acgaaacttt gaaaaaaatt gtggaatttc aggcgcagaa cgacaagtaa acgccattac
     2161 aatagcgatg tgcctgctgg caaaagccgg caggcatttt cctgtcccac cggcaataca
     2221 ggcagtaccc acacgcccca ggttccggaa ccaaagaaaa atttatgctc ctttcaccgt
     2281 ctcagtgtgg actgaaggga aggagcagta gtgaggtgga tggaatggaa caaggccgct
     2341 gggtcctgct gattgccagt gaagcaggcg ggactgattc ggtagcagac caggcgatgg
     2401 agcgcctggt gcaggcaatt caggaagaag ggtatcgcgt ggtgcgcacc tcgacgccgg
     2461 aagacgggct ggcgctggtg acctctgacc cttcgtacag cgcgattctg ctggactggg
     2521 acctgccaga gaacaatcag tttgacgagc gcgccgcgct gcgcattatc cgcgccgtgc
     2581 gccgccgcaa caagaagctg ccgattttcc tcattgccga ccgcacgctg gtgagcgaac
     2641 tgccgctgga agtgatcaag caggtccacg agtatatcca tctcttcgga gatacacctg
     2701 ccttcattgc caatcgcgtg gatttcgctg tggagcgcta tcacgagcag ttgctgccgc
     2761 cctatttccg tgaattggtg aagtacaccg accaaggggc gtattcctgg gatgctcccg
     2821 gacacatggg tggcgtggcc ttcctgaagc accctgtggg catggccttc cacaagtttt
     2881 ttggcgagaa ccttctgcgc tccgacctcg gcatctcgac ttcgcagctc ggatcgtggc
     2941 ttgaccacat cggccctccg gcggagtctg aacgcaacgc ggcgcgcatc tttggcgcgg
     3001 actggacctt ctatgtcctg ggtggttcgt ctacttcgaa ccagattgtg gggcatggcg
     3061 tgattgcgca ggacgagatg gtattggcag acctgaactg tcataagtcc atctgccact
     3121 cgctgaccgt gaccggagcg cgacccgttt atttcaagcc tacgcgcaat ggctttggga
     3181 tgatcgggct ggtgccgctc gcgcgctttc gtccggaaaa tattgcggag cagatcaaca
     3241 agagtccgtt tgttgccggg gccgtgtcaa agacgccgag ttatgcagtg gtcaccaact
     3301 cgacatatga cggattctgt tacgacgtga acgcggtcgt cgaggagctg aaaaagagcg
     3361 taccccgtgt gcattttgat gaagcctggt acgcatatgc taagttccat ccgctgtatc
     3421 gcggacgctt tgccatggac gtgccggacg agatgccgga ccgtccgttg ctgtttgccg
     3481 tgcagtccac gcacaagatg cttccggcgt tttcgatggc ttcgatgatc cacgtgaagc
     3541 ttagcccgcg cgcgccgctg gagttcgacc agttcaacga gtcgtttatg atgcgcggaa
     3601 cgacctcgcc gttctatccc atgattgcct cgatagatgt ggccacggcg atgatggatg
     3661 agcctgccgg ttcgacgctg atgatggaga cgatcgagga cgccatcagc ttccgcaagg
     3721 cgatggcctc ggtcgcgcac cggctcaaag ccgaggaggg tggcggctgg ttcttcggaa
     3781 tgttccagcc ggacacggtg accgatccgg acacgggtga agtttatcgc tttgaagacg
     3841 cgccggatga tctgttgtcg gaatcgccca aatgctggac tctcaagccc ggtgatgcgt
     3901 ggcatggctt ccgcgatgaa gacattgcaa atgattacgt gctgctcgac ccgacgaagg
     3961 tcacgattct gacgcccggc attgatgcgc atgggaacat gggcgagtgg ggcattcctg
     4021 ccgcgatcct gacggcattt ctggatgcgc ggcgggtgga gatcgcccgt accggagact
     4081 acaccattct gacgctgttt tccgtgggca ccagtaaagg caagtgggga agcctgctgg
     4141 agacgctgtt tgagtttcgc agactttatg acaccgaggc cccggtcaat gacgcgctgc
     4201 cgcagcttgt ggccaagtat ccggagcgct acggcatgat ggacctgaag cagttgagcg
     4261 atgaaatgca cgcggccatg aaggaactga acctgcctgg actgctgcag gaagcgttcg
     4321 atgtagacct gcctcaagtg atgacgccaa cgcaggcgta tcagcggctt atccgcgaag
     4381 ggacggaaaa aatccgtttg acggaaatgg ccggccggat ctcggcggtg atgctggtgc
     4441 cgtatcctcc aggaataccg atgtgtatgc ccggcgaacg tctgggagat gccaatagtc
     4501 ctacgatccg gtttctgctg gcgctggagg aattcggcaa gcgcttccca ggctttgagc
     4561 gcgaggtcca cggcattgaa gtggacagcg agggcaactt ctggatgcgt gccgtgattg
     4621 aaactcccgg gaagaagacc aacggcagaa gcaagccacg gacgcatccg tcctcgcggg
     4681 tggcgaaata gatatcgcgc aaccggaagg gtgggttatc tggagctgac aggcatggcc
     4741 tccagctctg cctgcgcttc tcgctggatc tctgccacaa tctggtaaga acgcagccgt
     4801 gccgcatgtt cgtatgggtt acaggtaagg atcaattcat cggcgcgagt tttttcaaga
     4861 aaagcttcaa gttttgtccg tactgtattg cggtcgccgt atatggccgc agcccgtttg
     4921 gatgtaattg cggcgtgttc caggtcgttt gccaggcctt ccacgctgtc caccggcggg
     4981 gaaagaggcg cgagctggct gcgaatcaat cgcagaacag tgatatattc cgtgcttgat
     5041 agccgctggg cttcttcatt tgtatctgcg gcaagtacag gcacacccac catgacatag
     5101 ggttcttcga gcacctcaga ggggcggaag taatgccggt aaatttcgag cgccgagaac
     5161 agatctgccg gggagaagtg agcggcaaag gcaaagggca ggcccaggtc gcccgcaagc
     5221 tgcgcgctga atccgctgga accgagtagc cagacaggga cattcagccc ggccccgggg
     5281 atggccttga ccgcttgacc cggtttcggt tccgcgaaat atccgcgaag ttcggcaacg
     5341 agctgcggga aattgtcgcc attgtgcaat tcgcgccgga gcgcccgcat ggtgggatag
     5401 tcggtgcccg gagcgcggcc cagtccaaga tcaatacggc cgggatagag gctttcgagc
     5461 gtgccgaatt gctcggcaat caccagcggg gcgtggttgg ggagcatgat tccgcccgcg
     5521 ccgacgcgga tggttgaggt ccctcctgca acataaccaa tcagcactgc ggttgccgcg
     5581 ctggccacgg atgcaagatt gtggtgttcg gccagccagt aacgcttgta tccccattgc
     5641 tcggcgtgct gcgcgagatc gagtgtctcg cggaagctgc cggagatgga atggccctct
     5701 tttatggggg caagatcgag aatggaaagc ggagtacggg caagccggga agcatcgggc
     5761 atgaagaaaa cctcattgaa aagatgctga ttctatcgcg catgattcag gctgatgggc
     5821 cgagggcctg agagtacagg caatccaata caagagaaca aaagcgaatc aggctgtgtt
     5881 gttttcgagt cgttcgcgct gacattactt ttttttgata cacttaaaga agtcggcagt
     5941 gcccacgccg gcctcttcat tcggcgccat ggtgtaattg gtcaacacgc cgccctctca
     6001 aggcggagag ttcgggttcg atccccgatg gcgctaccaa agaaatactc aattccgcag
     6061 tccgggtggc gcgaaatctt agcagtattg gctaaacctt caatcgctga gattcttcct
     6121 cccttcggtc gtcagaatga cggaatatcc ctgaaaaagt tataggtaat caggcagcat
     6181 ctgcttcagt gacgggttta ggttgaacag aagagggtgt ccaaagcctt tggaagtcac
     6241 gctctgatcc aagcaggaat gcacgagtaa ccgggaaccg ggcaaggaga agagtcacga
     6301 cccaggacga gataagggca acaagaaagg cagcccagcc gttcttagtc agccgggaaa
     6361 cgaggcccat ggacaagacc actttgtgga tgaccatgat tcccatggaa gatgctccga
     6421 ctgaggcgaa caccatagtc aatgctgctg tacgcgtgat gatacggctg agagcaatca
     6481 caacgagaat gcaactcaaa cccagtacaa gggagagtcc gggaactcca tagttgcctg
     6541 atttcatgtc gtagctgatg ggaacatgca agcgaacgag ccagacggtc aggatgaggc
     6601 ccggtattgc gagcagttcc aataagcgtt ggtgcgagct tttgcgatac agatatccag
     6661 cgaggagaag cggcaaggcc cccagcacga catgtgcgtt gagaggcagg gtgaaatgcg
     6721 gggccaaatc cgtaagcagg taagcgcctg tgagagagac caggccagcc acaaggacca
     6781 ttcccggtcg aaaccgtacc agcagataat ttgctatctg ttgggtgagg taaagacagg
     6841 tgaaaaacca caggacaccg taacggcctt ccatctgtcc accgccccag agaaatgttt
     6901 tcgtgagcgc gccccacttt tccgggtggt gaaaagcgct tatataagct agagggcaga
     6961 gcaacagcat gaaggtcacg taaggcagca gcaggtggat ggctttgcgc tttgcaaaaa
     7021 ggccataatc cggttggggg cgatgaagaa atccgctgag aaaaaagaac aagggcatgt
     7081 ggaaggtata gatgaagcgc tggagcgctg ttccatctat ggaatgcccc agtacaacgc
     7141 aaatgatgcc caggcctttg cagacgtcca cccacaggat gcgtccatca cgctcactca
     7201 ttcatgtccc ccggtgtaga gtcttcttca tcatagcgtg tacgcggcag gggacaaaga
     7261 ctgagcgcag aatcctgatg aataagaagg aggaggggag aatcttagca ggattggcca
     7321 gagccgcaca ttgctaagat tcttcctctc ttcggccgtc agaatgacgg actccacaaa
     7381 tacgaaacat gctataaacc ctgcgaactc cagagcgcgg tttactttac cttgacctta
     7441 ggaattacgg acattcccgg gcggaggaga tggtctgagt tctgattgtg gtcagtcaga
     7501 tctatgcgga ccggaatacg ctgcacgacc tttacgtaat ttccagtggc attttccggt
     7561 gggaagaggc tcagtacgga cccggttgcg ccgccaattt gtgtgacttt gccatcatac
     7621 ttgcgtccgc cataggcatc tacgctgatg gtgacgggtt ggcctgggcg catgtgtgcc
     7681 aactgtgtct ctttgaagtt cgccgtaatc cagacatcat cgagtgagac gagtgtggcc
     7741 atgttctggc caacgctgac gttctgaccg acttcaacac ttttcttcgt gatgattcca
     7801 gccacagggg cgatgattct ggtgtagccc aggttcagct ttgcctgttc aagcgcagct
     7861 cgtgcctgct gcacctgcgc ggccgcctgg tcagcgcggg ctttttgaat ggcgaccagt
     7921 tttggtccct gctgagcgta gctataagaa gcctgcgcgg aagcaactct gtctttggca
     7981 acgcgcacgg ctgcctgtgc ggctgccaga ttggctttgg cctggtccag agcggcctta
     8041 tcggcgtcgg cggtagcaac cgcggcatca tattgctgtt tggagatgac gtcacgctga
     8101 acaagcggcg tgtagcgttc gaggtcggcc tgggcctttt tgtaattcgc atcggcctgg
     8161 gcgacacctg cttcggcagc ctgcaattgt tgctgagact gcgctacctg cgcctcagca
     8221 cttgagacat cggccccagc tgaagacaga ttgctccttg tattgatgtg cgtgacagga
     8281 acgttgacat tggccgattc gtaggccgct tctgcggctg ccagattggc ttcgtcctgc
     8341 tgcacggcgg tctcaaagtc tttggggtcc agctcggcga tgacggtgcc ggcttccaca
     8401 tactggtttt cgtccacgtt gacttttatg acgtgaccgg caacgcgcgc gctgatctgg
     8461 atgaggtgcc cggagacctg cgcgtcatct gtgtcctcat aataggtgga gtgccaccac
     8521 cagaggaggg caatgacaac ggcgatagcg acgacgccaa tgatgatgaa ccgccgccgt
     8581 ggcttttgcc tctgctcttc ttccggctgg tcaatacggt tctgttcttc agtttgcact
     8641 gtggtctgct ccgccacttt actttcctcc gacgtaactc ttgtagttct tttcagccat
     8701 gcccaatgcg cgcgcaagcg agagcttggc aatattgtgc tggtacaggc tgctcacata
     8761 ctggtcgtct gcctgcgcga ctgcctgctg cgcctgggag acggcgaggt tgtctgaaac
     8821 tccggcggcg tagcgctgct gtgcttcgct cagggcctca tttgccagct gcacgttgct
     8881 gcgcgccact tccacctgct tctgtgctgc cttgatgtcg agaatgctat cgcggacatc
     8941 ggcgctgatc tggccttgca ggtcgcttaa ctgcgcacgt ttcgattcaa gctgggcctg
     9001 cgcctgacgc gcatcgccgc gcaactttcc ctcttcgagg accggaacag acagcgtgcc
     9061 ggttgcgttg cctgtgccat gagaatggct gagagtgggc ccgatgtcac cgtagtctcc
     9121 ggcaaaactg attgtgggat aacgctcggc agttgcggcg gaacgcgctt tttctgctgc
     9181 cctgagttgt tcctgcatgg ctttcagatc gttgcgatta gccagggcct gctttacggc
     9241 ggtttccgga tctatctcgt cgagcgccgc atagggggcc gtatcggtca gttcgaattt
     9301 ctgttccagt gggaggccga tgacacgcgc caatgcgatt ttgtcttttt cgtattggtt
     9361 ttgggcggtg atcaggttct gctcctgcgt ctgatagtcc acacgggcac gcaattcgtc
     9421 gagaagaggg gccgttccgg cacgatggtt ctgtactgcc tgatcgagag aaacctttga
     9481 agtgttcacc tgggcctgcg cgctgtctat gcgggccttg tcggcaatca ccaaaagata
     9541 ggcattgccc acggtgaggg tgaccatgtc tcgcgcatcc tgggcggaca gctttgcgct
     9601 ctggaagtta tgttttgcgg cgagatagtt ctgaagcgaa gccacgttta gcagcgacca
     9661 gttcagcgac gcacgcacat ctgtgtaccc gaacgggcca atgatttggg gaaaaccagg
     9721 gatgcgtaat ccctcagcag ccaggttgac ctgttgcacg gattccttca gcgatgcgct
     9781 ggcaaccggc agaagggcct gcaattcctg caggcgcgat ccgttggcgg aaagcgtgtt
     9841 ttcgttgctg agcacgagtc cgaggttgta cttcaggccg cgctggatgg cgtcgtcgag
     9901 cgacaggggg aggatgcctt ccgttggttt ttgctgcacg acgctaccct gaaaaccgct
     9961 gccgccctgc tgggccgccg gaatctgata ggggctgggc agtggcgatg aggaactgcc
    10021 tgtctgcgcc tgagccaata cggcctgggc gctccaaggg agcaaaaaca ggcccagggc
    10081 ggcactccac cgcctgttat tttggatgac acgcacgaaa cgattcacct tagtcccagg
    10141 aaagactctt acctaagaag attatggtct ttggatgctg taaatcgcag aaaaagttca
    10201 gaaaattgca gggaacaaag agcttaagcc atcaggcggg ttgtgccgtg cccgcctgaa
    10261 ccaaagaagt ggccagagag cagagcgcct ggcgaaagac atcgctgacc ttgtcggcgg
    10321 tccagcctgc ggcgccgctt cctccgctgg agcggagatc ttccgctgta gcggcgttat
    10381 tctgcccaag catggcgcgc tcaacgaaaa tggtgcgctg gatggccgga aggtgttcaa
    10441 gccagttgcg cagacggccg cggccttcac cttcgagaag ccaggtgagt tgctcgtgag
    10501 acaacccaga ggtggaaagg tcatcgctct caatgcagcc agcagacccc tggcctgctg
    10561 attccgaggc ggcgaatgct tctggttcgc gctggcttaa caagcgaatg gctgcccgga
    10621 caatcttatt ctgcacgagg gcgttggccg cttctccgtc cgcacaggga tctacttctg
    10681 ccgcggcgat ggcttcttcc accagggtga cggcatccgc ctcattgctt accagaaggg
    10741 cggcggtctg gaaaaacttt gctgctgtgt cctgcgccag cagttcgcta caaccctgat
    10801 ctcccataat gactaccccc gccgttactt ttatcctcgt tgatggggca gtcctgagtc
    10861 aaattccgac cggaccggcc ctcttttttc tgtttgttca ccacagatgg cttcttaatt
    10921 cggtaaactt tgcatctgat tctgtgattt ttatgagcac gcgttcgaga cagcagcaaa
    10981 gtcttttgat tggcagagat gaatcctgga tgcaatttaa ccgccgggtg ctggaggagg
    11041 cgcaggacgt ctctaaccct ctgctcgagc gggtgaagtt tctggcgatc acggccagca
    11101 atctggatga gtttgtggaa atccgggtgg cgggactgtt gcagcgaatt gaagacggct
    11161 acacggaggc tggtcctgac ggtatgactc cgcagcaaac gctggatgct ctgaatgagg
    11221 acatgcagtc gtttgtagtg gaacagtaca agtgctggaa ccagcaactg atgcctgctt
    11281 tgcgtcaggc gggagtgcgc gtgctgggct ggcacgagat ggatgagacg gcgcaggagt
    11341 ccgccacggc tttttatcag cgtgaagtag acccattgct gacgccggtg acgattgatc
    11401 cggcgcaccc ctttcctcgc gtgttgaaca aggcgctgtg catagcgtta ttgctgcggc
    11461 gcaagcgcaa gaacacagcc ggcccggtac tgggtgtcgt gacgattcca cgcgcattgc
    11521 cacggctggt tccgctgccg tcgaaccagg ggacgcatga ttacattttt ctgcatgacc
    11581 tgattgagcg ccatgcggcg ggaatgtatc gtggatacga aattctgtcg aaggcagcgt
    11641 ttcgtgttac gcgcaacagc aatctctact ttcaggaaga agaggcgcgg tcgctgctgg
    11701 aaactgtccg ttcagagctg cacaaccggc gcaaggggga tgcggtgcga ctggaaattg
    11761 agcagagcgc cgacccggag attattgacc gcctccgtat gaattttgag ctggatgaat
    11821 cacaggtgta tcgcacagat gggcctgtga acctgtcgcg gctgatgaat ctttacagcg
    11881 acactccgcg gccagacctg aagtttcagc cttttgtacc gcacgaattg cgcctgaacc
    11941 gcaaatcggc ggacctgttc gacgaactgc gtcaccgcga tattctgctg caccatcctt
    12001 acgattcgtt cgacgctgtg gtgagcttta tggaactggg agctgcggac ccgaatgtga
    12061 tttcgattaa acagacgctt taccgtacca gctccgattc agccattttc agcgcactga
    12121 ttgaggcggc acagacgaag gaagtcacgg ccgttgtgga gctgacggcg agatttgacg
    12181 aggcgtcaaa tatccgctgg gcgcgggaga tggaagacgc gggcgtgcag gtgtttcacg
    12241 gcattgttgg cctgaagacg cactgcaagc tggcgcttct ggtgcggcgc gacccagatg
    12301 gagccgtgcg gcgttatgcg catctgggca cgggcaacta caatcccaat acggcgcggt
    12361 tctatacgga catcagtctg ttgacagccg accctgcgat tacggcaggt gtccacagtg
    12421 tgttcaacta tctgactgcg cattcggagt cggacgatta tgctcctttg ctggttgccc
    12481 ctctgacgct ggcggaaagc atgattcgac tcatcgcgcg tgagacggag cacgcgaggg
    12541 cggggcggcc tgcgcgcatc atcgccaaga tgaattcgct gctggagcag agagtgattg
    12601 aggcgctata tgccgcttcc agtgccgggg tgcagattga cctgattgtg cgtggcatct
    12661 gctcgctgcg accgggactg aaagaaatca gcgagaacat tcgcgtgcgt tcgattgtgg
    12721 ggcgatttct tgagcacagc cgcatctttt acttcgccaa tggcggggag gaagagattt
    12781 actgcggcag cgcggactgg atgccgcgta atctgttcga gcgctgcgag gttgttttcc
    12841 ccattaagga tgcgcaactg cgcgcgcggc tgaggaacga gattcttgca gcttatcttg
    12901 cagacaccat caagacccgc atgctgcttg gcgatggaaa gtacttccga ttgcgcgaga
    12961 caacgctggg gaagtcgctg ccggcgttca gcgcacagga atttttcatg aaagtggctg
    13021 aagggaagac gactgccgac gatattccga agatgccgga agagcagaat ccaaagcctg
    13081 ctgtaaagaa gcgccggaag gtcatgacct ctagtgaaat gggttaaaag ttttcacgcc
    13141 gagagatgca ctggcttcgt gcatctgcca ctttatgggt gcctcttcta accctaaact
    13201 catctcacaa attcaattgt attgaaatcg aggccgggcc aaagccatag aatttggggc
    13261 ctattttacc gatctgaagg ctggtgcttc taccagaatt gtttatgaga ctgcttccag
    13321 tctgattcaa taacgggctt tctgctcatt cagagctgtg ctaacttgct tcagagcgaa
    13381 ttcattcgga gtcaacgatg gcctttactg agacgttcca ctgtgatgtg tgtggcaagg
    13441 agaagaatga ggaatcagag gactggtggc tggcatgggc ggaacgggtg tcgtctgttc
    13501 ctgatggggc aaacatccct cagctccgtt ttgttccatg ggacgtgctg ctttctcatg
    13561 atgcgcgggc aaggcacctg tgcggtgcgc gttgcgcaca gacactgatg gaccggtgga
    13621 tgacatcacg cggagaatct acggaattgc agattccccg ggagacttga actttttgta
    13681 gtctcctgat gcctgcgaag aaatgcaggt ccttggcgtt gctcaggatg aggtgcacat
    13741 gaatgtatcg ggagttgtcc cctacttcgg attggaccat ccgccgaaat catttgtgag
    13801 gcgggcggct tctttcgggc cccatgtccc tggcttgtag cgatgtactt cagggcggtc
    13861 tttgagcaca ttgtcaaaga tacgccatgc ctcttccacg tagtcctcgc gagcaaacca
    13921 tgcgggatta ccgcgcatgg cgtcgtccag taattcttcg tagggaagaa ggttgtcttt
    13981 tccgcattcc tgttctgcct caagctcgac catgcagccg cgcaggcggt cgcctgcttc
    14041 cttcacagaa ccgccaatag caatgaccgg cacggggctt aagcggaagc gcacgtagtt
    14101 ctggggcaga aggtcttctg aaaacacagg aggcgtctgc cgcagcctgg caatgacttc
    14161 agttgcggtg acgggcagcg attttccggt gcgaatgaag aatggcacat cgcgccaccg
    14221 ccatgaattg atatgcagac gcatggcggc aaaagtctcg acagtagagc cgggtttcac
    14281 gccaggctca ttcagataac cctcgaactg gcccagcacg agattgtcct tgtccatggt
    14341 ctgcacgctc ttgagtactt tcacgcgttc atcgcgcaga gcttccacgt caggattcgg
    14401 cggcggctcc atggcaatgt tgctgacgac ctgcatcaag tggttctgga cgacgtcgcg
    14461 gatggcgccc actccgtcat aaaacgcgcc gcggccctga acgccaaagc tttcggccat
    14521 ggtgatctgg acgctttcta catactggcg gttccagacg ggttcaagaa aaagatttgc
    14581 aaagcggaag aagatgacgt tctggacgga attttttccg aggtaatggt caatgcgaaa
    14641 gatattttgt tcgccgaaga cacggtggat ttccgctgtg agggcgcgcg ccgattcgag
    14701 atcgtggccg aagggttttt ctacaacgac acgcgcgcca ttggcgcatc cggaatcgcg
    14761 gagctgatca atgacgcgcc cgaagagaat gggaggaatc gccaggtagt gcagcggaca
    14821 ttttgcgtcg cccagctctt ttcttacctg ctggaaagta gcgttgtcgt tgtagtcgcc
    14881 atctacgtag cgcagttttt ccaggagccg tggcagtcct tcagcgtcgg cgccaccgtg
    14941 ggtctccacg ctgtcttttg cacgggcctt gagctggtcg agtgtccatc ctgccttggc
    15001 gacaccgatg accgggcctt gcagccggcc gcgtttggcg agcctttgca gcgcggggaa
    15061 tatctgtttg taagcaaggt cgccggttgc gccaaaaaat acaagtgcgt cagattgtgt
    15121 ctggtccatg tcgcctcagt gtttttgcct tcagtacttg gatgttggtt cctatgttca
    15181 ggaaacatga caaagagcaa gtctatcagt gtgggctttc tttcatacgt tgacaagatt
    15241 tgaatcgcag agtgggtgtc tggagttccg gttcagggtt tctttgcgag ttgaaaggcc
    15301 ttttccagca cagcaagggt gaagggagcg atgcggtatt ttccgtgcgc attcagctct
    15361 tcgcgcgagg cccatgcggc tgcaacggca tcggtggaac agtgaagctc gcctccggtg
    15421 agacggcaaa ggaaatctac cagaacataa tgaaagcgca ctgcgccttt ttcatcatgg
    15481 gcgatgcggt cgagtacttc aacgatctgg agtggctcga cttcaagacc ggtctcttcg
    15541 catacttcgc ggcggaggcc gtcgtgaagg gtctcgccga gttcaagggc gcctcctgga
    15601 atagaccact cgcctttgag cggctctttt ccacgctgga tgaggaggac tttttcttcg
    15661 tgaagaatga tggcccccac accgatgatg ggggtttcag gatattcgcg gcgcatattg
    15721 ggagattaca ggatacgtgg cccagttctt ccgctgagtt ccagcgaaga aagcaagtct
    15781 tccgccttcg gcccgggatg cgaatccgtg tggtgcagga ttacagcttg atgggtttgc
    15841 cgtattcctc ggcgaaaacg gtacgggcca tgctgaagcg tccgccgtag aatttgtcgg
    15901 ggcctttcag atggccgatg gcgagcaggg cgacgacgtg gtagctgagg ggcagcttga
    15961 ggacttcgcg cactttctct tcctcgaagc cttccatggg agcggtgtca tagccgagtg
    16021 cctcggccat aagcatcatg gtggtaaagg cgatcatgac gtggcggttg agccacatgg
    16081 ggaagttggg gtggttggaa aggtagttgg ggatgtttac tttggcctgc tcggcatagt
    16141 tttccggcat gccgccgtcg cggcccaggc gcagcatctc ttcgaggtcg ccgttgcgcc
    16201 agccatcggc gtctccgcaa gcgacgatga cggccgaggc ctcttctact tttgcctgat
    16261 tgaagctggc ggagcgcagg cggcgctttt gttcggggct gcgcaccacg atgaaacgcc
    16321 agggttgcat gttgtatcca ctgggcgcgt ggagtccggc gttcaggatt ttcttcaggt
    16381 cttcatccgg aacaggggag ccatcaaagc tgggggttgc gcggcgctgc gcgatcacct
    16441 gagataaatt tttctccatt atgtttgcca tgaagatttt tcctgaagtt tactggattt
    16501 ggttcattgg ctgttcaatg ccgaagggcc gctgatttgg ggcattggca tagacccaga
    16561 ggccatgaaa gccctcgcgc agttccggat gctgggcgag aagggccttc attacatcaa
    16621 tattgcggct gcgcgtagcg acgggatcag aggtgtctgt ggcgttgtaa tttaccacga
    16681 gatcgagctg gccgagtgag ccgtcagtgt gcaggttggt gatttcgtag gtctgcccgt
    16741 tggctgcgac cgtcatcggg ctggagccgg ggaggccatc aggcctggtg ctgttcatct
    16801 ctttctggag tttttcaagg ttcgggctgg agagaaaatc agcaggcagc gcgaggtaag
    16861 cagcggtttc gtaatagaag tatgcgttcc actgctgctt tttctttgca aaatcgcggg
    16921 cctgtgtcca gtaccagacg ctgtcgtgtc cggcgagcgt aagtggtcgc gcatagaagc
    16981 ccgccagctt ccactgtgtg cttcctgccg gatcattggc cagaatcagg gccatctgct
    17041 gcggctgttt tccgcctgcg gagtggacaa ggacaatggc gtaattcccg ggcggcagtt
    17101 gtgcgaggga gacagtgata agcacttcag agccgggaat gctgcaaaaa aactgcgtgt
    17161 ccgcgggtgc cttcaggtcg gaggcgttca gggcatatat gttctggacc gtgaaggttg
    17221 ccccttgcag gtctggcgcg atggactgga tggtgctggc gatgggatcg aactgcgcgg
    17281 cgacactgct gatggtattg gctttgatgg cagagacgtt tccggactgt atctctgtgg
    17341 ccagagtgtg cgcggcctgt acgaaggcag agcgctgagg gtcggtcatc tgggattggg
    17401 tggtgcaggt gacggcgctg gcgattccgc cagtaagaag gacagccgga aaaaggaacc
    17461 atcgccaacg attcataggc cgaaaagaaa attccgcaag caaattgaaa ccactgctag
    17521 tctagtacat agagggattc cagcaggagt cctatagctt cgagatgcgt tttagctttt
    17581 cattctggat gatggctgct gtggtggcag gcgcgcagac agcgcctcca cctcatcgtg
    17641 tccctggggc aaagcctgtt gccgcggttt cagagcagcc tgcgcctgtt gttccagcgg
    17701 ggccgtcttc gttactggac caacctgcac agccggccca tgttcaattt gatggccata
    17761 gactctcagt gaaggcagat aattcgacac tttcgggcat tctaaatgag atttcctcga
    17821 agacggggat gaaggtagac gggccctcgg gagaccagc
//
LOCUS       DASRBV010000175         9733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_263890, whole genome shotgun sequence.
ACCESSION   DASRBV010000175 DASRBV010000000
VERSION     DASRBV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263890"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFA02_06630"
     CDS             complement(<1..1163)
                     /locus_tag="VFA02_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZU09563.1"
                     /translation="MDLFHENPNPMWIYDTETLRFLDVNEAAIRAYGWSHEEFLSMTI
                     LEIRPSEEFQAVRSSAKRNDPVQSSGPWRHRYKDGSERLVRITSHAVEFNSRPARAVT
                     VTDVTDSVRATEALRISEAGLRALTDRLPVALLYIDAQGNCRSANRTFEDWFNISRDE
                     IVQRNFEFFIRRGDGVQYEADVRRSWSLALNGQPSTFEKTIYIHGRKRNIEASYTPDF
                     DAEGQVCGVIILLHDITDRRALEEQFRHAQKMEAIGRLAGGIAHDFNNVLSVINGYAG
                     ILQQELKQNHRLGHMASEIHRSGERAARLTQQLLAFSRKQVSQPRVVRLADLLKNDEV
                     MLRRLMGEDVHLEIKAGPETIAIEMDPHRFSQVLMNLAVNARDAMPKGGTLTIA"
     gene            complement(1303..2631)
                     /locus_tag="VFA02_06635"
     CDS             complement(1303..2631)
                     /locus_tag="VFA02_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HZU09564.1"
                     /translation="MRINFYLFKSTIVGALGGLLFGFDTAVIAGTTHQLTEVFQLSAF
                     ALGVTVSIALFGTVIGAMSAGAVGQKLGSRETLRIMAVLYVVSALGCAFAWNWPSLIA
                     FRFIGGLGIGGSSVLGPVYIAELAPAKWRGRLVGLFQINIVIGILLAYASNYLIARLD
                     LGLLQWRWELGVAAAPALLFLIMLYGIPRSPRWLTTQNRIDEARQVLQLMGSPDSEAE
                     MREIIDSIHLERSERSEPLFQYKYRLPIFLAISIGMFNQLSGINAILYYMNDIFRAGG
                     FSAVSGDLQAVLVGAMNLVATLLAMTVIDRLGRKTLLLIGSVGTALCLAGVASIFLLG
                     QHQDYLVWFLVAYIFFFAISQGAVIWVYISEVFPTRVRSKGQGLGSSSHWIMNALISL
                     TFPVLAKHSSGTPFVFFAVMMVLQFFVVLFIYPETKGHTLEQMQYRLGIE"
     gene            complement(2708..3739)
                     /locus_tag="VFA02_06640"
     CDS             complement(2708..3739)
                     /locus_tag="VFA02_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZU09565.1"
                     /translation="MNSFSIGVDLGGTNLRIAAYTRASGLLETILLPTRLAAGRHAVV
                     EDICSGVRKLLHTYSSQHYLAGIGIGTPGPLELPVGRLHSPPNLPGWDGFGLREEVES
                     SLGMPVTVESDANVAALAECILGSGRTLGLSSLCMLTLGTGIGNGIILNGKIWHGVNG
                     MAGEAGHVTIEPDGPACGCGSNGCLEMYASATAVRRIALDAAESGKAPAIAALAKRNP
                     DFTTRDLSDLAEQGDPDAKRIFDGVGRALGIGIADLVNTLNLPLYVVGGGLANAWDLF
                     SPALFAEVRHRSYVYRLTEPGRSVLNGGATNGTRIMSAELGADSGLLGACILPYTLNR
                     EKQGSALSS"
     gene            complement(4084..5136)
                     /locus_tag="VFA02_06645"
     CDS             complement(4084..5136)
                     /locus_tag="VFA02_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU09566.1"
                     /translation="MIKPGSHPTLADVARKAGVGTTTVSRVINGGHRVSPRTLKRVHA
                     VIEQLGYMPNQAARILKGERTKTIGLVVPSIADPFFSSCAEAAQEIARAHDSLLIVTV
                     TNNDPHIEMENLNVLMRHRTDGLLLAPANYQSEALAEFLGRISTPVVTFDRPVAKAGV
                     PSVVTDNYNGARIATEHLVSHGYKRILCLGGEPTLYTLRERLRGYRHAIEEHGLPVIV
                     DMSVKDYKSAEYAIESHLATSQPPEAIFTLKNSTTIYAFETLQKLRVAIPKSIALLGF
                     DDFELASTLRPSISVIQQPIEDLGRIAAEILFEQLLPAKKSKYPSPKKRQDQVKLETR
                     LVLRHSCGCNPTQEPA"
     gene            complement(5170..6093)
                     /locus_tag="VFA02_06650"
     CDS             complement(5170..6093)
                     /locus_tag="VFA02_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU09567.1"
                     /translation="MARRPPFSFIVNILARSAFRPMKGTQHQPVLAAPGQTAISFIGH
                     SSFLIQMSGLNLLVDPVFANWLLVLRRIRRPGIAIKDLPPIDTVLLSHAHMDHLNLPS
                     LRKIIAHTRQLSGKAPEVIVPWGVADLVARLGFRKVHVLEWWQNMHLGHIEIVMTPAK
                     HWGARTMKDTHRGYGGYVLRNSVHSIYHSGDTGYFKGFREIGERLAPHIALLPIGAYS
                     PDGFRAVHTSPEDALQGFADLAAATMIPMHYGTFCLSAEPVEEPLPRLLSAARKNGVE
                     DRVLPLAEGQTRIFSPVPAYAQEELTSIHRA"
     gene            6216..7244
                     /locus_tag="VFA02_06655"
     CDS             6216..7244
                     /locus_tag="VFA02_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZU09568.1"
                     /translation="MCSLLLAISATAQEAHKWAVVLHGGAGVIERKNMDAKTEAAYRA
                     SLQAALQKAADTLDKGGSALDAVEAAIRMMEDDPLFNAGRGAVFTAAGRNELDAAIMD
                     GSNLKAGAVAGVTRTRHPISLARAVMEKSPHVMLIGAGADEFAATHGLEMVDPGFFFT
                     ERRWDALVKELKKEGRPIPPRPAGAPPAPGKPVVEIELPDAHKFGTVGVVALDKQGNI
                     AAGTSTGGLTAKMWGRVGDSPIIGAGTYADNRSCAVSGTGTGEYFIRLTVARTICALV
                     QYKGMGLQEAADEVIQHQLTDMKGDGGVIAIDTKGDMVWSFNSPGMYRARVSEGGKPQ
                     IEMYKDQP"
     gene            7433..8008
                     /locus_tag="VFA02_06660"
     CDS             7433..8008
                     /locus_tag="VFA02_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZU09569.1"
                     /translation="MDHLWTPWRYSYITTADTAKRQGVPKELSAWQGDTGCVFCNMLE
                     SVKYAESHGMVPELAEKAALIVHRGEHIFICLNRYPYTSGHVMVLPYTHESSLAALPA
                     DVAHELMDAAQILERVLEDVYRPEGTNMGLNLGKAAGAGVAGHLHLHVLPRWIGDTNF
                     MTTVGETRVLPEDLETAWKRIREGLGKRLRA"
     gene            8310..8699
                     /locus_tag="VFA02_06665"
     CDS             8310..8699
                     /locus_tag="VFA02_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU09570.1"
                     /translation="MTFDLSPYVDWSLLLLRIMVGIVFITSGYGHLKAPRKRAESIGM
                     SLGFTAFLGVAELLGGIAVIIGFLTQWAAVGLILIMLGAIYMKIAKWKTGFWGEKSSG
                     WHYDLMFIVMNLVILCTDGGYFSLMRR"
     gene            8938..9594
                     /locus_tag="VFA02_06670"
     CDS             8938..9594
                     /locus_tag="VFA02_06670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU09571.1"
                     /translation="MSLRINDVAPDFTAETTQGTIRFHEWIGDNWAIFFSHPKDFTPV
                     CTTELGAVASLQNEFAKRGVKVLGLSVDPVEDHHKWAKDIEDVGGHPVKYPIVGDPEL
                     KIAKLYDMLPATAGDTAKGRTPADNAPVRTVFVIGPDKRIKLTLSYPMATGRNFDEIL
                     RVIDALQLTTRHKVSTPANWKQGEDIIISGAVSNEEANKLFPGYKTVKPYLRTAAQPQ
                     "
     gene            9643..9718
                     /locus_tag="VFA02_06675"
     tRNA            9643..9718
                     /locus_tag="VFA02_06675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9676..9678,aa:Cys,seq:gca)
BASE COUNT         2184 a         2557 c         3029 g         1963 t
ORIGIN      
        1 gcaatggtaa gtgtgccgcc tttgggcatg gcatcgcggg cgttcacagc caggttcatc
       61 aaaacctgac tgaagcggtg cgggtccatc tcaatggcga ttgtttccgg cccggctttg
      121 atttcgagat ggacatcctc gcccatgagc cgccgcagca tgacttcgtc attcttcaga
      181 agatcagcga gacgaacgac gcgcggctgg ctgacctgtt tgcggctgaa ggcgagcaat
      241 tgttgggtga ggcgtgctgc ccgttcgccg gagcggtgaa tctcggacgc catgtggccg
      301 agacgatgat tttgcttcag ttcctgttga aggattccag cgtatccatt gattacagaa
      361 aggacattgt tgaagtcatg ggcgatgccc cctgccaggc gtccgatggc ttccatcttc
      421 tgggcatgac gaaactgctc ttcaagagcg cgcctgtcgg tgatgtcgtg cagcaggatg
      481 atgactccac agacctggcc ttcggcgtcg aagtccggcg tataggatgc ctcaatattt
      541 cgtttcctgc catggatata gatggtcttc tcgaaggtcg aaggctggcc attcagggcc
      601 agagaccatg agcggcgcac atcggcctcg tactgtacgc catcaccgcg gcggatgaaa
      661 aactcgaaat tgcgctggac aatctcatcg cgcgagatgt tgaaccagtc ttcaaaagtg
      721 cggttggcgc tgcggcagtt tccctgcgcg tcaatataga gcagggcgac agggaggcgg
      781 tcagtcaggg cgcgaagccc agcctcgctg atgcggagcg cttccgtggc gcgaacagaa
      841 tcggtgacat cggtgacagt gaccgcgcgc gcggggcggg agttgaactc gactgcgtgc
      901 gaggtgatgc ggacgaggcg ctcggagccg tccttgtacc ggtgccgcca ggggccggaa
      961 gactggacgg gatcgttccg tttggcactc gaccgcacgg cctgaaattc ttcggatggg
     1021 cgaatttcaa ggatggtcat gctgaggaat tcttcgtgcg accagccata tgcgcggatg
     1081 gcggcctcat tcacgtccag aaaccggagg gtttccgtgt cataaatcca cataggattg
     1141 ggattttcgt gaaagagatc caccgaaatg cctctcggga gctgctgaat gaccgggtga
     1201 gggacagttc gactgtatca cgttgggcca gaggcgggga atttcagggc agaacccctg
     1261 tgcagtccaa accagcaggc tacacttcgg gcacccggtc ctttactcaa tgccgaggcg
     1321 atattgcatc tgttcgaggg tgtggccttt tgtctccgga tagataaaca gcacgacgaa
     1381 gaattgcagc accatcatta ccgcgaagaa gacaaagggt gttcccgagg agtgtttcgc
     1441 caggacgggg aaggtgagcg agatgagtgc gttcatgatc cagtgcgaag agctgccgag
     1501 gccctggccc ttggagcgca cgcgcgtcgg gaagacctca ctgatataga cccagatgac
     1561 cgcgccctgc gagatggcaa agaagaagat gtaggcaacc agaaaccaga ccagataatc
     1621 ctggtgctga cccagcaaaa aaatggaagc aacgcctgcc agacagaggg cggtgccaac
     1681 ggagccgatg agcagaaggg tcttgcgacc gagcctgtca atgacggtca tggccagcag
     1741 tgttgcgacc agattcattg cgccgacgag cacggcctgc aggtctccgg agacggcgct
     1801 gaaaccgccg gcgcggaaaa tgtcattcat gtagtagagg atggcattga ttccggagag
     1861 ctgattgaac atgccgatgg agatggcgag aaaaatcggc agacggtact tgtactggaa
     1921 gagcggttcg gagcgctcgc tgcgttccag atggatggaa tcaatgattt cgcgcatctc
     1981 agcctcagaa tcgggcgatc ccatgagttg cagcacctgg cgggcctcat caatgcggtt
     2041 ctgcgttgta agccagcggg ggctgcgcgg aattccatag agcatgatga gaaacagcag
     2101 cgcaggagca gcggcgacgc caagctccca gcgccattgc agcaggccga ggtcgagacg
     2161 cgcaatcagg taattgctgg cataggcgag cagaatgccg atgacaatgt tgatctggaa
     2221 gagaccgaca aggcgtccgc gccacttcgc tggggccagc tcggcaatgt atacggggcc
     2281 gagtacggag gagccaccga tgccaagtcc tccgatgaag cgaaaggcga tgagagaggg
     2341 ccagttccag gcgaaggcgc aaccaagcgc cgagacaaca tacagaacag ccatgatgcg
     2401 cagcgtctcg cggctgccga gcttctgacc cacggccccg gcggacatgg caccgatgac
     2461 tgtgccgaag agagcaatgg aaacagtgac gcccagcgcg aatgcagaaa gctgaaagac
     2521 ttccgtcagc tgatgggtgg tgcccgcgat gacggcggtg tcgaatccga agagaagtcc
     2581 gcccagcgcg cctacgatgg tgcttttaaa aaggtagaaa ttaattctca tgcctgcgtg
     2641 ctttctactc cccatacaag tccaaccgag ggcctatatg gggcatcctg agagattcgt
     2701 tttgaaatca ggaagacaat gcagaacctt gtttctctct gttcagggta tagggaagaa
     2761 tacatgctcc cagcagtcct gaatcagcgc caagttctgc ggacataata cgtgtgccgt
     2821 tggttgcgcc gccattcaaa accgagcggc ctggctcagt caggcggtag acatagctgc
     2881 ggtggcgcac ttcagcaaaa agcgcgggcg aaaagaggtc ccatgcgttc gccagacctc
     2941 cgccgacaac gtacagcgga aggttcagtg tattaaccag gtcggcaatg ccgatgccca
     3001 gggcgcgccc tactccgtcg aagatgcgct ttgcgtccgg gtctccttgt tcggccaggt
     3061 cggaaagatc gcgggtcgta aaatcagggt tgcgcttcgc cagagcagcg atggccgggg
     3121 cctttcctga ttcggcggca tcgagcgcga tgcgccggac cgcagtggca gaggcataca
     3181 tttccaggca tccgttgctg ccgcatccgc aggccgggcc atccggttca atggtgacgt
     3241 ggcctgcctc tcctgccatg ccgtttacgc catgccagat ttttccgttg aggatgatgc
     3301 cgttgccgat gccggtgccc agggtcaaca tgcaaagaga gctgaggccg agggtccgtc
     3361 cactgcctag gatgcattcg gcaagcgcag ctacattcgc atcgctttct accgtgaccg
     3421 gcattcccag ggaggattca acttcttcac gcaggccaaa gccgtcccag ccgggcagat
     3481 tcggtgggct gtgcagacgt ccgacaggca gctcaagcgg gccgggtgtt ccgatgccga
     3541 ttccggcgag atagtgctgc gaggaatatg tatgaagcaa tttgcggaca ccgctgcata
     3601 tgtcttctac gacagcatgg cgtccggcag ccagacgagt gggcaaaagg atggtctcca
     3661 aaaggccaga ggcacgggta taggccgcga tgcgaaggtt tgtaccacct aaatccacac
     3721 caatcgaaaa agaattcata aatagttaaa aagccctaac agacagacgg caaagtatgt
     3781 aagttgccgc ggcatgcaat taaaaaataa tgcgatgcgc ggcgctggaa gcagcctttt
     3841 gcaatataca cgaaaaaagg caaaggtgga aacgattcca tcggcgacct ggagatcagg
     3901 gttcagagca caagatcccg ggtccatggc ccttcaggag tcagcgcaca gcccaaatta
     3961 ggacaaccgc ttgtctgtcc atggacaaac catggtcgcc gaaagaacgg gccgtcgttt
     4021 gccattgcgc cccaactgcg cttttcgttc ccgggccggc acccgagaga ggagggcggt
     4081 acctcaggca ggttcctggg tggggttgca gccacaggaa tggcgcagca ccagcctggt
     4141 ttcgagcttt acctggtcct gccgcttttt cggagacggg tatttgctct ttttggcagg
     4201 cagaagctgc tcgaagagaa tttccgcggc aatacggccc aggtcttcga tgggctgctg
     4261 gatgacgctg atagaggggc ggagggtgga ggccagctcg aaatcgtcaa aaccaagaag
     4321 ggcgatgctt ttgggaatgg ccactctcag cttctgaagt gtctcaaagg catagatggt
     4381 cgtgctgttc ttcagcgtaa agatggcctc aggaggctgc gatgtggcaa ggtggctctc
     4441 gatggcatat tcggctgact tgtagtcctt caccgacata tcaacgatga cgggcagacc
     4501 gtgttcttcg atggcgtggc gatatccgcg aaggcgttcg cgcagagtat agagcgttgg
     4561 ttcgccgccg aggcagagaa tgcgcttgta gccgtgactg acgaggtgct cggtcgctat
     4621 gcgcgcgccg ttgtagttgt ccgtgaccac gctggggacg ccggcctttg cgacgggacg
     4681 gtcaaatgtg acaacgggag tcgagatgcg gcccaggaac tcagccagtg cttcgctctg
     4741 gtaatttgcc ggggcaagca ggagaccatc ggtgcgatgg cgcatgagca cgttcaggtt
     4801 ttccatctcg atatgcggat cattattggt cacggtgaca atcagcaggg aatcgtgggc
     4861 gcgggcgatt tcctgggcgg cctcggcaca gcttgaaaag aatgggtcgg cgatgctggg
     4921 aacgactaac ccgatagtct tggtgcgctc gcccttgagg atgcgcgccg cctgattggg
     4981 catatatcca agctgctcaa tcacggcatg gacgcgcttg agtgtgcggg ggctgacgcg
     5041 gtgtccgcca ttgatgacgc gcgaaacggt ggtagtgcct actccggctt tgcgtgcgac
     5101 atcggcgagc gttggatgcg agcctggttt gatcatcgtg cgttcacgat tacagctttg
     5161 caccagtatt caggcacggt ggattgatgt caattcttcc tgtgcgtatg cggggacagg
     5221 ggagaagatt cgcgtctggc cttcggcaag aggcaggaca cggtcttcca ctccgttttt
     5281 tcgggccgcg gagagcaaac ggggcaatgg ctcttccaca ggctctgcgg aaaggcagaa
     5341 cgtgccgtag tgcataggta tcatggttgc ggcggccagg tcggcgaagc cctgcaaggc
     5401 atcttcaggg ctggtatgca cggcgcggaa gccatccggc gaataggccc cgatgggaag
     5461 cagcgcaatg tggggcgcta gccgctctcc aatttcgcgg aaccctttga aataaccggt
     5521 atcgccggaa tgatagatgg aatggacgct attgcggaga acgtatccgc catagccgcg
     5581 atgtgtgtcc ttcatggtgc gcgcacccca gtgtttcgcg ggagtcatga cgatctcgat
     5641 atggcccagg tgcatgttct gccaccattc cagaacatgg acttttctga acccgaggcg
     5701 agcgaccagg tcggcaactc cccagggcac gatgacctca ggcgctttgc cggaaagctg
     5761 ccgcgtgtga gcaatgatct tccgcaaaga aggcagattc aggtggtcca tatgcgcatg
     5821 ggagagcagg accgtatcta tcggcggtaa atctttgatg gcaattccag ggcggcgtat
     5881 tcgacgcaaa acaagcagcc agtttgcaaa gaccgggtct accaggagat tcagcccact
     5941 catctgaata agaaacgacg aatggccaat gaagcttatt gccgtttgac cgggcgcggc
     6001 cagaacgggc tggtgctgcg tacccttcat ggggcggaaa gcggaacggg caagaatgtt
     6061 cacaataaag ctgaaaggcg ggcggcgtgc cacaggttct cctgagaaaa ggatgccgga
     6121 gaaagcaaaa aagatgcgga gcgaggtggc gttgcatggt ggataggcgt ctatgccggc
     6181 gctatgattc cgacatgaga aaatccatac tcgctttgtg tagtttgctt cttgccatct
     6241 ccgcaactgc gcaggaagcg cacaaatggg ccgtcgttct ccacggtgga gcgggcgtga
     6301 tcgagcgaaa gaacatggat gcaaagacgg aagcggcgta tcgcgccagt ttgcaggcgg
     6361 cgctgcaaaa ggcggcggac acgctggaca agggcggttc cgcgctcgat gcggtcgagg
     6421 cagcgattcg catgatggaa gatgaccctc tcttcaatgc aggacggggt gccgtcttta
     6481 ctgctgcggg cagaaatgaa ctcgatgccg ccattatgga tggctccaat ctgaaggccg
     6541 gagcggttgc cggagtgaca cgcacgcggc atcctatctc gctggcgcgg gccgtgatgg
     6601 aaaaatcgcc gcacgtgatg ctgattggcg caggcgctga tgaatttgcc gcgacgcatg
     6661 gcctggagat ggtagaccct ggattctttt ttaccgagcg gcgctgggat gctctggtaa
     6721 aggaactgaa gaaggaaggc agacccattc cgccgcgtcc cgcgggtgct ccgcctgctc
     6781 ccgggaagcc cgttgtggaa atcgagctgc cggacgcaca caaattcggg actgtgggtg
     6841 tggtagcgct cgataagcaa gggaacatcg ccgcgggaac gagcacaggc gggcttacag
     6901 cgaagatgtg gggacgcgtt ggcgactcgc ccatcattgg agcggggact tatgcggaca
     6961 accgctcctg cgccgtctcc ggaacaggga cgggcgaata tttcatccgc ctgacggtag
     7021 cacggaccat ctgcgcgctt gtgcagtaca aggggatggg ccttcaggaa gcagccgatg
     7081 aggtcatcca gcaccagctc acggacatga aaggcgacgg cggcgtgatt gcaattgata
     7141 caaagggcga tatggtgtgg agcttcaatt ctcccggaat gtatcgcgca cgcgtttccg
     7201 aaggtggaaa gccgcaaatt gaaatgtata aggaccagcc gtagcgcatt tttcgctgcg
     7261 cggagaatcc tggcaggatt gtctgaagcc cggcatcgct gagattcttc ctcgctgcgg
     7321 ttgtcagaat gacgggctct gcagatacaa gaaatgccga tgatgaagaa catcagagag
     7381 ctgcgctgtc ggcatgtctt cagtaccggt gcagtcgtta tactcaaaac ctatggacca
     7441 tttgtggaca ccgtggcggt acagctacat cacgaccgct gatacggcaa agcgccaggg
     7501 cgtgccgaaa gagctttccg catggcaggg agatacaggc tgtgtatttt gcaacatgct
     7561 ggaatcggtg aagtatgccg agtctcatgg catggtcccc gaactggcag agaaggccgc
     7621 gctcattgtg catcggggag aacatatttt tatttgcctc aaccgttatc catacacctc
     7681 agggcatgtg atggtcctgc cctatacgca tgaaagctct ctggcggcgc ttccggccga
     7741 tgttgcccac gagctgatgg atgccgcaca aatacttgaa cgcgtgctgg aagatgtata
     7801 tcgcccggaa gggaccaaca tgggactgaa tctgggcaag gccgccgggg caggagttgc
     7861 cgggcatctg catctgcacg tgcttccacg ttggattggc gacacgaact ttatgacaac
     7921 ggtaggagaa acgcgcgttc tgcccgaaga cctggaaaca gcatggaaac gcattcgtga
     7981 aggacttggg aaaagacttc gcgcataaat cactccagtc ccgattcaaa actgagaaaa
     8041 ctccatccgc tgcgcggcgg cctacttgca gagtgaagag gagattttat gcctaacgca
     8101 tcggatgtgc tggtcgggcg cctttccagg cgctggcggc acggacggcg gattatctga
     8161 tggagcaggg cggccgtacc ttctttccca atgaccgccc gcaggcaggg ccgaaaattc
     8221 gttacgaccc cagtcctcca tttacaaagt tcaagggcaa tcccagggtt gcataacagg
     8281 ccgcggcatc caaatcctat ggaggtttta tgaccttcga tctttctcca tacgtggact
     8341 ggagcctgct cctgctgcgc atcatggtgg gcattgtctt tatcaccagt ggatacgggc
     8401 acctgaaggc gccgcgcaag cgcgcagaaa gtatcgggat gagcctgggc tttacggctt
     8461 ttcttggggt ggctgagctg ctgggcggga ttgcggtgat cattggcttc ctgacgcaat
     8521 gggccgcggt agggctgatt ttgatcatgc ttggcgccat ttatatgaaa attgcgaaat
     8581 ggaagaccgg cttctgggga gagaaatcct ccggatggca ttacgatctg atgttcattg
     8641 tgatgaacct ggtaattctg tgtacggacg ggggatattt cagcctgatg cgccgctagc
     8701 catcattcat ttgacatggc ccagaccaac cgtctatgct tgaggtcaag ttgatgaaaa
     8761 gcgtcgctcc aacctgcatg tgtatctgct gctgctgttg ctgctgcacc gtgcgggtgt
     8821 gttcgtagag cgaagccggt agtaccaagc tcaaacgccc acccgcagga aatctgctga
     8881 ggtgggtttt ctatttcaca ggaaagattg gggttcccct gaagaggaaa aatcgttatg
     8941 tcacttcgta tcaatgacgt tgcaccagac ttcacagccg agacgaccca gggcaccatc
     9001 cgcttccatg agtggattgg cgacaactgg gccatcttct tctctcaccc gaaggacttc
     9061 acacctgtct gcaccactga gcttggcgct gtcgccagcc ttcagaatga gttcgcaaag
     9121 cgcggcgtaa aagtgctggg cctgagcgtg gaccctgtcg aagaccatca caagtgggca
     9181 aaggacatcg aagatgtggg cgggcatccg gtgaagtacc ccatcgtcgg cgatcctgag
     9241 ctgaagattg ccaagctgta tgatatgctg ccggcgacgg cgggcgatac cgccaaaggc
     9301 cgcacgcctg cggacaatgc ccctgtgcgc acggtctttg tcatcgggcc ggacaagcgc
     9361 atcaagctga ctctctcata cccgatggcc acgggccgca attttgacga gattctgcgc
     9421 gtgattgatg ctctgcagct taccaccagg cacaaggtct caacacctgc caactggaag
     9481 cagggcgagg acatcattat cagcggcgcg gtctcaaacg aagaggcgaa caagctcttc
     9541 cccggctata agaccgtaaa accgtatctg cgtacagcag cgcagcctca gtaaaacaaa
     9601 ggcgattgca tgtccgccgc gggccactgc ggcggacgaa gggcaccggt ggcgaagtgg
     9661 ctaacgcgct ggtctgcaaa accagtattc gcgggttcaa ttcccgcccg gtgctccaga
     9721 taagtcttct gcg
//
LOCUS       DASRBV010000176         6848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_161096, whole genome shotgun sequence.
ACCESSION   DASRBV010000176 DASRBV010000000
VERSION     DASRBV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161096"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VFA02_06680"
     CDS             complement(<1..1238)
                     /locus_tag="VFA02_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZU09572.1"
                     /translation="MATVLAGSARQLKEKIESRTARIGIVGMGYVGLPLALLFSEERF
                     RVTGFDIDPRKVEILNQGGSYIVRIPGTEIQSAKEKGFHATSDYSEIRDMDVVIICVP
                     TPLNEFHEPDLSFIRDTAKAIAPEVHEGQLIILESTTYPGTTEELLVPVLENENCAGL
                     KVVREPGASGFYVAFSPEREDPGNDTVARRDIPKVVGGVGPEALQLASAVYSSIFNRT
                     VPVSSPAAAEMTKLLENIYRCVNIALVNELKQLCHRMGIDIFEVIDAAKTKPFGFHAF
                     YPGPGLGGHCIPIDPFYLSWKAKQYDFQTKFIELAGEVNLAMPYYVVEQTAEALNSQK
                     KPLNGSKVLILGMAYKKDIDDLRESPSLTIIELLRQKGAIVSYNDPFFPYVGRGRHYN
                     LDMHCTPLDDLSQFDCVLIAT"
     gene            complement(1858..1934)
                     /locus_tag="VFA02_06685"
     tRNA            complement(1858..1934)
                     /locus_tag="VFA02_06685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1898..1900),aa:Arg,seq:tct)
     gene            complement(1925..2485)
                     /locus_tag="VFA02_06690"
     CDS             complement(1925..2485)
                     /locus_tag="VFA02_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09573.1"
                     /translation="MEADWSVEIGKGLPVIAAPWEGWIDLRKAPSRIREIPEALANPS
                     LAQALAHLNSPQSPVFTSKCDRWHLPADEIDPFEFDASQEESRTGLACYVDILLRDQN
                     AFASFGAHERWVLSASAALRQLPLPQCRADFVLRPAVFAHQEGFGITLYIAACSRNQA
                     TAQSIFESALNRTIAITIETSPSVGE"
     gene            complement(2463..3569)
                     /locus_tag="VFA02_06695"
     CDS             complement(2463..3569)
                     /locus_tag="VFA02_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU09574.1"
                     /translation="MSGMKAFLKLAANPGANGWCADFVPRRFLNNGHLMTLAGNFLPR
                     HHAPLPQPEALRVEVQGPMPGYEEYGPTSIVCHCNWQPERVRSQRLTVVLVHGLEGSS
                     SSQYMLGNAARAWAAGCNVVRINMRSCGGTDKLSPTIYNSGCWHDVALVVERIVSEHH
                     LHAIAVIGYSMGGNLVLSYAGQFGNNPPPFLKAAVGISPLMDLAASSAALHEPQNRIY
                     EKYFLRSMIARLHRKAELFPRLYFRLYADRVLEKIHSMRDFDEYVVAPYGGFTSADDY
                     YYSVASSNVASALSIPTLIIHSLDDPFIRMLPETRQALIDNPYVSFIETPYGGHCAFL
                     APANGYDGYWAEKTLLGFLLATSESEANGSRLVG"
     gene            complement(3566..4285)
                     /locus_tag="VFA02_06700"
     CDS             complement(3566..4285)
                     /locus_tag="VFA02_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZU09575.1"
                     /translation="MHGTVYLGSSGWAYPSWKPDFYPSKLPAKKFLEFYATQLNSVEV
                     NYTFRALPSATMAQSWLAATGPDFRFSFKAPQRITHILRLNNCTEALSAFFRSLQPFI
                     DANRIGAVLFQLPPNFKADPERLDAFLEKAAATNLHPAFEFRHESWFADEIYTILRKH
                     NAALCVAENDELATPDIVTAPFSCYRFRKSSYSAEDLHQIRNLLTKQAGQGDVFAYFK
                     HEEDPSGAMHAATLLQELRPA"
     gene            complement(4292..4366)
                     /locus_tag="VFA02_06705"
     tRNA            complement(4292..4366)
                     /locus_tag="VFA02_06705"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4332..4334),aa:His,seq:gtg)
     gene            4400..4933
                     /locus_tag="VFA02_06710"
     CDS             4400..4933
                     /locus_tag="VFA02_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HZU09576.1"
                     /translation="MKPQVLPPDRGNLHARTKLGNRLFADENLDLLARVLDEWFRIPG
                     TSIRFGLDGIVGLVPWAGDVLAGLASCVIIMAAWFRGVPYVTLMRMVVNLGVDVIIGA
                     VPVLGDAFDIAWKANRRNYKLLSRHLDEPHRHTWKDWVFLLLIAFVVAAIFMVPLFLL
                     ILAMEWVAHRMVLVGHP"
     gene            4967..5043
                     /locus_tag="VFA02_06715"
     tRNA            4967..5043
                     /locus_tag="VFA02_06715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5001..5003,aa:Pro,seq:tgg)
     gene            complement(5069..6010)
                     /locus_tag="VFA02_06720"
     CDS             complement(5069..6010)
                     /locus_tag="VFA02_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU09577.1"
                     /translation="MGPSISPRLFHCLLSVCFLLFLTSCGSSTVHSSSGPQHIQNVFI
                     IMMENHNWTGAGANSIQNNPNAPYINNTLNTLVPMGAHASNYNNPPHMHPSLPNYLWL
                     EAGTNFGILNDGPATTHSQSTTMHLVTLLKNAGISWKAYDEGTDGTQCLFIHWHVPFV
                     FFDDVTNNLDPHSAYCISHIRPFSELYSDLAANTTARYTFIVPNDCHSMHSSCNGGNQ
                     IAQGDEWLSQIVPQILASNAYKNGVLFIIWDEAEKGDGPIPFIVLSPFAKMGYTNTTY
                     YNHGSTLRTVEEIFGVQPLLRDASNETDLSDFFRQFP"
     gene            complement(6083..>6848)
                     /locus_tag="VFA02_06725"
     CDS             complement(6083..>6848)
                     /locus_tag="VFA02_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HZU09578.1"
                     /translation="PVSCMWIFFQTVDAWERRSPMLLQTVGICIAFGLIVWLTRAATT
                     PAVFTGTMMTACLYLRIPGWRTALWPLVAMLVLTLAATRFGRSRKLELGLAESRSGRS
                     ASQVAANLGIAALACIPAIPTAVALAGIAAALTEAAADTLSSELGQVFGGQPMLLTTF
                     QPVPAGTDGGITLAGTVCGCLGAAILAVISAATFHFGLSWAAAIFSCGVLGLFVDSLL
                     GATLERRGMLNNDAVNFLSTLISAASAAWLANRFFC"
BASE COUNT         1516 a         1601 c         2213 g         1518 t
ORIGIN      
        1 gtggcgatga ggacgcaatc gaattgagac agatcatcga gaggcgtgca gtgcatgtcc
       61 aggttgtaat gacggccgcg gcctacatag gggaagaagg gatcgttgta ggagacaatg
      121 gcgcctttct gacggagcaa ttcaatgatg gtcagggaag gcgactcgcg caggtcatca
      181 atgtcttttt tgtaggccat gcccaggatg agcacttttg atccgttcaa cggcttcttc
      241 tgcgaattga gcgcttcggc agtttgttct accacgtaat agggcatggc gaggttgact
      301 tcgcccgcca gttcgatgaa cttggtttgg aaatcatact gttttgcctt ccaggagagg
      361 tagaaggggt caatggggat gcagtggccg ccaagcccgg gtccgggata aaaggcatgg
      421 aagccgaagg gcttggtctt ggctgcatca atgacctcga agatatcaat acccatacga
      481 tggcaaagct gtttcagttc attgaccagg gcaatgttca cgcagcggta gatgttttca
      541 agcagcttgg tcatttcggc agccgccgga gaggaaacag ggacagtgcg gttgaagatg
      601 ctggagtaga cggcagaggc gagttgaagc gcctcgggtc cgaccccacc tacaaccttg
      661 ggaatatcgc gccgtgctac ggtgtcattt ccggggtctt cgcgctctgg ggagaaagcg
      721 acataaaagc cagaggcgcc gggttcgcgt accactttga gtccggcaca gttttcgttt
      781 tccagaaccg gaaccagaag ctcttcggtg gtacctggat aagttgtgct ttcgagaatg
      841 atcagttgtc cttcgtggac ctccggagca atggcttttg ctgtgtcacg gatgaagctg
      901 aggtccggct catggaactc gttgagcggt gtcgggacac agatgatgac gacatccatg
      961 tcgcgaattt cggaataatc cgaggtggcg tggaaacctt tttccttcgc ggattgaatt
     1021 tctgttcccg gaatacgaac gatataggag ccgccctggt tgaggatctc tactttacgt
     1081 ggatcaatat cgaaaccggt aacgcggaaa cgctcctcgc tgaagagcag agccagcggc
     1141 aggccgacat atcccatgcc tacaatgccg atacgggcgg tccttgattc aatcttttcc
     1201 ttcagttgac gggcgctgcc agcaagtacg gtcgccatga actcggttcc tccagagaca
     1261 cacaagatat catgccagat ggcatggacg cattggaggg agcaattgga gggccatccg
     1321 tcttgcgggg cgacatctat cgttcttttt cccgtggtac acgtgtttcc cacggggaat
     1381 acccgactct aaaaaaatgt aaattcttca aaatacaggg cttatgtaat tattgtcctg
     1441 caaattcctg aaaataaatg agtttcgttt aaattcctga ttttaaaatg tttgcggccg
     1501 ctcgtccata gactgtctat ggattgcgtg aatttggggc gttttcatca ggtaagggcc
     1561 agatacagtt ggtacgcgtt tctgacccac gttaaccggc gaagtctttt gacttcgctc
     1621 ctgctcacat ggggcacccg ttctttttgc ttctctttct ttcaggatat caggttgaga
     1681 gggaatgtcc gacggtgcca ggatgtggct atgttattgg atacgttgca ttaagccgga
     1741 gatgttactt ccgtaagggt tgacagggag tcggggcggg aacgcggcca gtgacaagcc
     1801 cttcgatgtt cagggtggcg atttttacag gcagccgggg aaccgggttt gaaagtctgg
     1861 taggcgagac agggatcgaa cctgtaaccc ccggcttaga aggccggtgc tctatccaat
     1921 tgagctactc gcccacggaa ggggatgtct ctattgtaat ggcgattgtg cgattcagcg
     1981 cagactcaaa gatggattgc gcagttgcct ggttgcgaga gcaggctgcg atgtagagag
     2041 tgatgccgaa accttcctga tgggcgaaaa ctgccggacg gaggacgaag tccgcacggc
     2101 actggggcaa gggaagctgt cgtaatgcag cggatgcgga gagcacccat ctttcgtggg
     2161 caccgaagga tgcgaaggcg ttctggtcgc gcaggaggat gtctacgtag caagcgaggc
     2221 cggtgcggct ttcttcctgc gatgcgtcga attcgaaagg gtctatttcg tccgcgggca
     2281 ggtgccagcg gtcgcacttg gaggtaaaga cgggggattg aggggagttg agatgggcaa
     2341 gcgcctgggc cagcgacgga tttgccaatg cttccggaat ttccctgatg cgggagggag
     2401 cttttctcag gtcaatccat ccttcccagg gggcggcgat gacgggaagt cctttaccga
     2461 tctcaaccga ccagtcggct tccattggct tcactttcgg aagtggcaag caggaacccg
     2521 aggagggttt tttccgccca gtagccatcg tatccattgg caggagcgag aaaggcgcag
     2581 tggccaccgt acggagtctc gataaaactg acatacgggt tgtcaatgag cgcctgacgg
     2641 gtttcgggca acatgcgaat gaagggatcg tcaagcgaat ggatgatgag cgtggggatg
     2701 cttaatgcag atgccacgtt ggagctggcg acggagtagt agtaatcgtc ggcactggta
     2761 aagcctccat aaggcgcgac gacatattca tcgaagtccc tcatgctgtg gattttttcg
     2821 agcacgcggt cagcatagag gcggaaatag aggcgaggaa acaactcggc cttgcgatgc
     2881 aaccgcgcaa tcatggaacg caggaagtac ttctcgtaga tgcggttctg cggctcgtgc
     2941 agagcggcgg aagaagcggc caggtccata agcggagaga tacctacggc cgctttgaga
     3001 aacggcggcg gattgtttcc gaactggccc gcgtagctga ggacgagatt gccacccatg
     3061 gaatagccaa tgacggcgat ggcgtgcagg tgatgttcgc tgacaatgcg ctcgacgaca
     3121 agggccacat cgtgccagca accggagtta taaatcgttg gtgagagctt gtctgttccg
     3181 ccgcaactgc gcatgttgat gcggacgacg ttgcatccgg cagcccaggc gcgggcagcg
     3241 ttgccgagca tgtactgcga gctggacgaa ccttccagtc cgtggacaag aacgacggtg
     3301 aggcgctggc tgcggactcg ttccggctgc cagttgcagt ggcacacgat gctggtgggg
     3361 ccgtattctt cgtatccggg catagggccc tgtacttcga cgcgaagggc ctcgggttgg
     3421 ggcaagggcg cgtggtggcg gggcaggaaa tttccggcca gcgtcatgag atggccgttg
     3481 ttgaggaagc ggcgcggcac aaaatcagca caccatccgt tggcacccgg gttggcggcg
     3541 agcttgagga aggccttcat cccactcatg ctggacgcag ctcctgaagc aaagttgcgg
     3601 cgtgcatggc gccggatggg tcttcctcgt gtttgaagta ggcgaaaacg tcgccttgtc
     3661 cggcctgctt tgtgagcaga ttgcgaatct gatggaggtc ttctgctgag tagctgcttt
     3721 ttctgaagcg gtaacaggaa aacggcgcag tcacgatgtc cggcgtggcc agctcatcgt
     3781 tctccgcgac gcagagggca gcgttgtgtt tgcgcagaat ggtgtatatc tcgtctgcaa
     3841 accaggattc gtggcggaac tcaaaggcgg ggtgcaggtt ggtggctgcg gccttttcaa
     3901 ggaaggcgtc gagccgttca gggtcagctt tgaaattggg cggcagttga aagaggacag
     3961 cgccgatgcg gttggcatct ataaaaggct gaagcgagcg gaaaaaagcg gacagggcct
     4021 cggtgcagtt gttcaggcgc aggatgtggg tgatgcgctg gggcgctttg aaggagaagc
     4081 ggaagtccgg gcctgtggcg gcgagccagg actgggccat tgtggccgag ggaagcgcgc
     4141 gaaaagtgta gttcacttcg acagagttga gctgcgtcgc gtagaactcg aggaattttt
     4201 ttgcgggaag cttcgaggga taaaaatcag gcttccagct tgggtaagcc cagccggagg
     4261 agccgagata aaccgttccg tgcattcggg ttggggcggc tagtggggat cgaacccacg
     4321 acatcctgag ccacagtcag gcgttctgcc gctgaactat agccgccgta caggtattga
     4381 ttgtagcatc tttcgttata tgaagccgca ggtattgccg cccgaccggg gaaatcttca
     4441 cgcaagaaca aagctgggaa accggctctt tgccgacgaa aatcttgatc tgctggcgcg
     4501 cgtgctggat gagtggtttc gcattccggg gacttccatt cgctttgggc tggatggaat
     4561 tgtcggactg gtgccgtggg cgggcgatgt gctggcgggg ctggcttcat gcgtgattat
     4621 tatggcggcg tggtttcgcg gagtgcccta cgtcacactg atgcgcatgg tggtgaacct
     4681 gggggtggat gtgattatcg gcgcggtccc tgtgctgggg gatgcgtttg atatcgcatg
     4741 gaaagcgaac cggcgcaatt acaagctgct gtcgcgacac ctggacgagc cgcacaggca
     4801 tacgtggaag gactgggtct ttctgctgct gatagcgttt gtggttgcgg cgattttcat
     4861 ggtcccgttg ttcctgctga ttctggcgat ggagtgggtg gcgcacagga tggtgctggt
     4921 gggacatcct tgaggcgtgt ggtcttcctg tacaatgagg aagagtcggg gcgtagcgca
     4981 gtctggtagc gcatctgctt tgggagcaga gggtcggggg ttcaaatccc tccgccccga
     5041 ccagtctcag tccattcggg aacaggctct acggaaattg cctgaagaag tcactcagat
     5101 cagtttcgtt ggaggcgtct cgcagaagcg gttggacgcc gaatatttcc tcgacagtgc
     5161 gcagagtgga gccgtggttg taatacgttg tattggtgta tcccattttg gcaaagggag
     5221 agagcacgat aaacggaatg ggcccgtcac ccttttcagc ttcatcccag atgatgaaaa
     5281 gcactccatt tttgtaggcg tttgaggcaa ggatttgcgg gacgatctgg ctgagccatt
     5341 cgtcgccctg tgcaatctgg tttccgccat tacaagagct gtgcatgctg tggcaatcgt
     5401 tggggacgat gaaggtatag cgggcggtgg tgttcgctgc caggtcgctg tatagctcac
     5461 tgaaagggcg tatatgggag atgcaatagg ctgagtgcgg gtcgagatta ttggtcacgt
     5521 catcaaagaa gacaaagggt acatgccagt ggatgaacag gcattgagtg ccatcggtac
     5581 cttcatcgta ggccttccat gagatgccag catttttgag caatgtgacc aggtgcattg
     5641 tggtgctctg gctatgagtt gtggcgggac catcattcaa aatgccgaaa tttgtacctg
     5701 cctccagcca cagataatta ggcaggctgg gatgcatgtg agggggatta ttgtaattgc
     5761 tcgcgtgagc tcccatggga acaagggtgt taagggtgtt attgatgtaa ggagcgttgg
     5821 gattgttctg gatactgttg gcgccggcgc cggtccaatt atggttttcc atcatgataa
     5881 tgaagacgtt ctgaatgtgc tgcgggccgg aggacgaatg gactgtcgaa gatccacacg
     5941 aggtcaggaa gagcagaaaa cacaccgaga gaagacaatg aaacaatcgt ggagagatgg
     6001 aaggacccat agaaatcctc cagtgtttgt ttagatgacg agtagcaaag gcgaagagta
     6061 ctttctcaat gggaagttgt tcttagcaga agaagcggtt tgccagccac gcagcagatg
     6121 cagcagagat gagagtcgag agaaaattca ctgcatcatt gttcaacatg ccgcgccttt
     6181 cgagggttgc tccgagaagg ctgtccacaa acaggccgag gacgccgcag gagaagatag
     6241 cagcggccca tgacaggcca aagtggaatg ttgctgcgga aatgacagca aggatggctg
     6301 cgcccaggca cccgcagact gttccggcga gggtaatacc gccatcggtc ccggcaggaa
     6361 caggctgaaa ggtggtcaat agcatgggtt ggcctccaaa gacctgaccc agttcggagg
     6421 acaaggtatc ggctgcggct tcggtgagcg cggcggcgat tccagcaaga gcaactgccg
     6481 ttggaatggc aggaatgcag gcgagtgctg cgatgccgag gtttgccgct acctgcgatg
     6541 cggagcggcc cgagcggctt tcagcaagcc ccagttccag tttgcggctg cgtccgaagc
     6601 gcgtggccgc gagcgtcagg acgagcatgg ccacaagcgg ccacagtgca gtgcgccagc
     6661 ccggtatacg caagtagagg caggcggtca tcatagttcc ggtgaaaacc gccggggtgg
     6721 tcgcggcgcg ggtaagccag acaatcaggc caaaggcgat gcagattccc acagtttgca
     6781 gaagcattgg actacgacgt tcccacgcgt caaccgtctg aaagaatatc cacatgcagg
     6841 agacaggc
//
LOCUS       DASRBV010000177         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_619381, whole genome shotgun sequence.
ACCESSION   DASRBV010000177 DASRBV010000000
VERSION     DASRBV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619381"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFA02_06730"
     CDS             complement(<1..206)
                     /locus_tag="VFA02_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09579.1"
                     /translation="MLASLGESIDRLNAAAAALERTAAFLEETRTVEKITAMVEDREQ
                     ELLRKLEAAEQQIADLRAQAQRPAA"
     gene            complement(178..798)
                     /locus_tag="VFA02_06735"
     CDS             complement(178..798)
                     /locus_tag="VFA02_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09580.1"
                     /translation="MRLEAMAIAMPEVAEHPNRVPFEGVLTVVDEVSTRPPSGARGHC
                     VVLTRRAAEEALPSLLGMAVDFAPKWDRHDARAKCGIITEADVVGKELRVRGYLFGRD
                     FPDVVQQIAGTAAGAMGMSYELADAHVADMLAEVWTLTKATFTGAAVLLREKAAYQNT
                     SFRLLDNHRSSSRLPQRLAAGTSYFYQHERERNGNRKHACFAGRKH"
     gene            complement(1068..2288)
                     /locus_tag="VFA02_06740"
     CDS             complement(1068..2288)
                     /locus_tag="VFA02_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZU09581.1"
                     /translation="MALKITDSIQKLWRRKEMGIAAEAAGSAKRKTLALPSILTPYRA
                     SSHSLPKPTPVNLRRFAETPLVRRAINLLKDRIASMDWQVRLKRGFSAAEISDAQARM
                     QILRQLLEEPNPADSFRTLMEQVLEDALVGGFGAIEMELTGNPEKPVEMWAVDGATVQ
                     IDPKWDGNPGSVRYAQTTGLPGDEGRILLRDDQLIYLRMNPRSHTPFGLGPLEVAFET
                     VNSFLAAHRFAGNLASNSVVQYALWLNETTPAEHERLIRWWQDEIEGTGRVPLLSTEQ
                     KPEVLRFANGTDADLRLAWQEFLIRMVANAFGLPPMLLGLEREVNRTTAAEQADEAFR
                     SAVVPLAKLVAEHLTRDLFAKKLGWREFEFVFNELDAPDEVAEMQIQTELLKAGVLTV
                     NEVRAMRGLAPLPE"
     gene            complement(2377..3210)
                     /locus_tag="VFA02_06745"
     CDS             complement(2377..3210)
                     /locus_tag="VFA02_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HZU09582.1"
                     /translation="MNERLQCDFLLVRYVPDPVRNEFVNIGVLLREAGRADRVLLRFT
                     KDWARVRCVDPDADIGMLEGLESELREKIGGELAPGKPLMQVLEDSLSNAVQITNPAA
                     YLAESMQAGIEELMRIYVEPRKREATARRSGRLAIHAQMRSQFERAGVWDLMRKRIAV
                     SAYTRAGDPLRIDCGYRPNGIIRMFHAVSLEGDSDLPKVLAFSAPAIREGVARVEKAE
                     LELTAVVEPVADVSEDREDSERVEQYRFAVETMETQRIRVLTVNDLPRMAETARRELR
                     V"
     gene            complement(3264..>3950)
                     /locus_tag="VFA02_06750"
     CDS             complement(3264..>3950)
                     /locus_tag="VFA02_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol kinase"
                     /protein_id="HZU09583.1"
                     /translation="CDVVEVTNWLIENSKDMIMDFGRHQEQCRAGLQFGSRLVGGLMP
                     GQVMDYLPEAQLADVKNLREFAGMLVIDKWTCNANGRQAVFYKRPREKKYAATFIDQG
                     YCFNAGEWKLVDAPLRGVYARNLVYRDVVDWDSFEPWLTRVEEMDPQKVWAVAEMVPP
                     EWYGGDVAEIERLIEQLLERRGRVRELITQFRESSRQPFPNWMKAKRVVVGRQFEEPS
                     WADEIRGPVM"
BASE COUNT          779 a         1341 c          945 g          885 t
ORIGIN      
        1 gccgctgggc gctgtgcctg cgcgcgcagg tcggcgatct gctgttcggc tgcttccagc
       61 ttgcgcagca gctcctgctc gcggtcctcg accattgctg tgattttctc cacggtccgt
      121 gtttcttcaa gaaatgctgc ggtccgctcc agcgccgcag ctgcggcatt caggcgatca
      181 atgctttctc ccagcgaagc aagcatgttt tctgtttcca ttacgctccc tttcgtgttg
      241 ataaaaatat gaagttcctg ctgccaacct ttgcggcaga cggctgctgc tccggtggtt
      301 gtccagaagc cggaacgatg tattctgata cgctgctttc tcgcgcagca ggaccgctgc
      361 tccggtaaat gtggccttcg tcagcgtcca gacctcggcc agcatgtctg ccacgtgagc
      421 atcggccagc tcataagaca tacccatcgc gcccgcggcc gtgccagcaa tttgctgcac
      481 gacatcagga aaatcgcggc caaacagata accgcgcacg cgcagttctt tgccaaccac
      541 atcggcctcg gtgataatgc cgcacttcgc gcgcgcgtcg tgccggtccc atttcggcgc
      601 aaaatccact gccatcccca gcagtgacgg cagcgcctct tccgcggccc tgcgcgtcag
      661 caccacgcaa tgcccacgcg cccctgaagg gggccgtgtg ctcacctcgt ccaccacagt
      721 cagcacccct tcaaagggca cgcggttggg atgttccgcg acttccggca tggctatcgc
      781 cattgcttcc agtctcattc cctctccatc actcgtattt gcacttgatt tttgttgttg
      841 aacagagcag tagcagtcag cgcctgctgc gcaggctgtc agcgcgcctt tcggcgctgt
      901 tagctaaaaa gcggttggcg gcctctggcc tgttagcacc ggatgatcca tcctgtcgcg
      961 ctgcttttgg gcgatagggt gggaatttca caaatattcg tcggctattc atttttcagc
     1021 caccagtccg aatcccgctc ttccgctgac ctgctgagtc actgctctta ctccggcaac
     1081 ggagccagcc ctctcatcgc gcgtacttcg ttcactgtta ggacaccggc cttcagaagc
     1141 tccgtctgta tctgcatttc tgctacttca tcgggagcat cgagttcgtt aaatacaaac
     1201 tcgaattcac gccagcccag ttttttcgca aacagatcgc gcgtcagatg ttcagccacg
     1261 agcttcgcga gcggaacaac cgcactccgg aaggcctcat cagcctgttc cgccgcagtc
     1321 gtgcggttca cctcgcgttc gaggccaagc agcatcggag gcagcccgaa ggcattcgcg
     1381 accatgcgaa tcagaaactc ctgccacgcc agccgcaggt ctgcgtccgt tccgttggca
     1441 aaacgcaaca cctccggttt ttgctccgtg ctcaacagcg gaacgcgtcc tgtgccttcg
     1501 atctcgtcct gccaccagcg aatcaggcgt tcatgttccg cgggagtggt ttcattcagc
     1561 cacagcgcat actgcactac cgaattgctt gctaaatttc cggcaaagcg gtgcgccgcc
     1621 agaaacgaat ttaccgtttc aaatgcaacc tccagcggcc ccaatccgaa cggcgtgtgc
     1681 gagcgaggat tcatccgcag ataaatcaac tgatcatcgc gcagcaggat gcgtccctca
     1741 tcgccaggca gcccggtcgt ttgcgcatag cgtaccgacc caggatttcc gtcccatttg
     1801 ggatcaatct ggactgtggc gccatccaca gcccacatct cgaccggctt ttccggattg
     1861 cctgtaagct ccatctcgat agcgccaaag ccgcccacca gcgcatcttc aagcacctgt
     1921 tccatcagcg tccgaaacga atcagccggg ttcggctctt ccagcagctg ccgcagaatc
     1981 tgcatccgcg cctgtgcgtc agaaatttcc gctgcggaaa atccgcgttt cagccgcacc
     2041 tgccagtcca tgctggcaat gcggtctttg agcagattga tggccctgcg caccagcggg
     2101 gtctccgcaa accggcgcag gtttaccggc gttggcttgg gcagggaatg cgatgacgca
     2161 cgatagggcg tcagaatcga aggcaaagcc agcgttttcc gcttcgccga accagccgct
     2221 tctgccgcga tccccatctc cttccgccgc cacaatttct gaatcgaatc cgtaattttc
     2281 aacgccatta cctccgatca ctttttccgc aaaaagaaaa ggcatgactc gaaagccatg
     2341 ccttttgtat tgacccgcac ctgcggactt gctatctcac actctcaact ctcgccgtgc
     2401 cgtttccgcc atgcgcggaa gatcgtttac cgtcagcacc cgaatccttt gcgtctccat
     2461 tgtctccacg gcaaagcggt actgctcaac gcgctcagaa tcttcgcggt cctcgctgac
     2521 gtctgcaacc ggctcgacca ctgctgtcag ttccaactcg gccttctcca cacgcgccac
     2581 gccttcccgg attgctggag cgctgaatgc cagcaccttt ggtaaatcag aatcgccctc
     2641 aagcgaaaca gcatgaaaca tgcgaatgat gccattcggc ctgtatccgc aatcaatccg
     2701 cagcgggtct ccggcacgcg tataggctga aaccgcaatg cgcttgcgca tcaggtccca
     2761 tacaccagcg cgctcgaact gcgagcgcat ctgcgcatgg atggccagcc tgccgctcct
     2821 gcgcgctgtt gcttcccgtt tacgcggttc aacatagatg cgcatcagct cttcgatccc
     2881 agcctgcatg gactcagcca ggtacgccgc cggattcgta atctggacag cattcgacag
     2941 agaatcttcc agcacctgca tcagcggctt tcccggagca agttcgcctc cgatcttctc
     3001 ccgcagctcg ctctccagtc cttcgagcat tccgatgtct gcatccgggt ccacgcagcg
     3061 cacgcgtgcc cagtcttttg taaaacgcag caatacgcga tcagcgcggc ctgcttcccg
     3121 cagcagcact ccgatgttca caaactcatt cctcaccggg tccggcacat accgcaccag
     3181 caggaaatcg cattgtaagc gttcgttcat acagatatct gtctctggct cgccttcggc
     3241 gccgttagct gactcgctgg ccctcacatt accggtccac ggatttcatc ggcccaactt
     3301 ggctcttcaa actgccttcc cacgacaact cttttcgctt tcatccagtt tggaaacggc
     3361 tgccgcgacg attcccgaaa ctgcgtaatc aactcgcgca cgcggcctcg tctttccagc
     3421 aattgctcaa tcaatcgctc gatctccgcc acatcgccgc cataccactc cggcgggacc
     3481 atctccgcaa ccgcccacac cttctgcggg tccatctcct ccactcgcgt cagccacggc
     3541 tcaaaactgt cccaatctac tacatcgcga taaaccagat ttcgcgcata tacaccccgc
     3601 agcggagcat ccaccagctt ccactcgcct gcattaaaac agtatccctg atcaataaag
     3661 gttgctgcgt atttcttctc acgcggacgc ttataaaaca ctgcctgccg tccgttcgca
     3721 ttgcaggtcc acttgtctat caccagcatt ccggcaaact cgcgcaggtt cttcacgtcc
     3781 gccaactgcg cttctggcag ataatccatc acctgtccgg gcatcagccc gcccaccagg
     3841 cgcgatccaa actgcaggcc cgcgcggcac tgctcctgat ggcgcccaaa gtccatgatc
     3901 atgtctttgg agttttcaat cagccagttg gtaacttcaa ccacatcgca
//
LOCUS       DASRBV010000178         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_9882,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000178 DASRBV010000000
VERSION     DASRBV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9882"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFA02_06755"
     CDS             <1..829
                     /locus_tag="VFA02_06755"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZU09584.1"
                     /translation="AHEAGIKLSVDDFDRISRNTPLLCDLKPGGKYVAADYYNAGGSR
                     LLAARLIEGGFLHKDCINVSGHTLAEEATQAKEAPDQDVIHPVNKAIKPTGGLVILKG
                     NIAPEGCVIKVAGHNRLEHRGPARVFDCEEDAFAAVEHGKIRPNDVLVIRYEGPRGGP
                     GMREMLQVTAAIVGIPELSETVALLTDGRFSGATRGLMAGHVSPEAAMGGPIAAIREG
                     DIIHFDIPNRRLDVEISEKELQSRLQNWKAPEPKFQRGVFRKYADTVSSASLGAVTN"
     gene            908..2641
                     /gene="ilvB"
                     /locus_tag="VFA02_06760"
     CDS             908..2641
                     /gene="ilvB"
                     /locus_tag="VFA02_06760"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZU09585.1"
                     /translation="MSSTPQTTRLTGSEITWATLVGEGVTDVFGYPGGAILPIYDAMR
                     KFPIRHILVRHEQGATHMADGYARASGKVGVAMATSGPGATNMVTGLATAMMDSVPIV
                     CITGQVSSKVLGTDAFQEVDITGITLPITKHNYLVTRAEDIAPALREAFLIAKSGRPG
                     PVLVDITKDAQQATAEFNFEAAAPKPYRPHPMRGHSADSISDAARLIREAKRPVILAG
                     HGIIQSDAYEQVRTLAERMQIPVASTLLGLGAFPASHELSLGMMGMHGEAWVNNAIQN
                     ADLLIACGMRFDDRVTGTLATYAPNAKKIHIEIDPAEINKNVKVDVALVGDLKQVLEV
                     LLPQIPSKSSSPWVREINEMKGECAVRDIKNLPDNGHLYAAHVINDLWNATGGKAIVV
                     TDVGQHQMWEAQYYKHDGPRTLVTSGGLGTMGFALPAAIGAKVACPEKEVWVIAGDGG
                     FQMTAAELSTIAQENFDINIAVINNGYLGMVRQWQEFFYEKNYASSPILSPDFVKLAD
                     AHGIAGATVTKRSEVLPAVESAREKKGPFLINFLVEKEDSVYPMIPQGKALHEMIRRP
                     DNPLIETAEDA"
     gene            2812..>3311
                     /gene="ilvN"
                     /locus_tag="VFA02_06765"
     CDS             2812..>3311
                     /gene="ilvN"
                     /locus_tag="VFA02_06765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZU09586.1"
                     /translation="MLHTFVALVEDKPGVLTRVASLFRRLNVNIVSLTVGRSEREGVS
                     RMTIVAEASPTAGHRITASLYKLENVLEVDDVGQFACVTRELALIKVAATPQTRSHIF
                     ELVEVFRARIVDLATESLMIEITGVSSKIEGLIQVLMESGDEILEVARTGRMVMRRGR
                     HTSRVM"
BASE COUNT          738 a          888 c         1029 g          656 t
ORIGIN      
        1 tgctcatgag gcgggaatca aactctcagt agacgatttc gaccgcatca gccggaacac
       61 tccgctgctc tgcgacctga agccaggcgg caagtatgtt gcagccgatt actacaatgc
      121 aggcggaagc cgcctgctcg ctgcacggct gatcgaaggt ggtttcctgc acaaggactg
      181 catcaacgtc tccgggcaca cgctggcaga agaggccacg caggcaaaag aggcccctga
      241 tcaggatgtg attcatccgg tgaacaaggc catcaagccc acaggcgggc ttgtgattct
      301 gaagggcaat atcgctcccg agggctgcgt gattaaagtc gcaggccaca accgtctgga
      361 gcaccgcgga ccggcccgcg tctttgactg cgaagaagac gcctttgccg cggtggagca
      421 tgggaagatc cgcccgaacg atgtgcttgt gattcgctat gaaggtccgc gcggcggacc
      481 aggcatgcgc gagatgcttc aggtgacggc ggcgattgtc ggcattccag aattgtctga
      541 gacagttgcc ctgctgacgg atgggcgctt ctccggcgca acgcgcggac tgatggcggg
      601 ccatgtttct cctgaggcag cgatgggcgg gccgattgcc gcgatccgcg aaggtgacat
      661 catccacttc gatattccga accgcaggct cgacgtggag atttccgaga aagagttaca
      721 gtcacggtta cagaactgga aggcgccgga gccaaagttt cagcgcggcg ttttccggaa
      781 atatgcggac acggtttcgt cggcatcgct gggcgcggtg acgaactagg tttgtaaatc
      841 ccggaactga ttcaccacag aggacgcaga gaagacgaag cggcttctaa agagggggaa
      901 cattatcatg agctcaacac cgcagaccac tcgccttacc ggttcagaaa tcacctgggc
      961 aacgctggtt ggtgagggcg tgacggatgt ctttgggtac cccggcgggg ccattctgcc
     1021 catctatgac gcgatgagga agtttcctat ccgccatatt ctggtgcggc acgagcaggg
     1081 cgcaacgcac atggctgacg ggtatgcacg cgcatcgggc aaggtcggtg tagcgatggc
     1141 gacctccggt ccaggcgcga ccaacatggt taccggactt gcgacagcga tgatggactc
     1201 ggtgcccatc gtctgcatca cggggcaggt ttcctctaaa gtgttgggta cggatgcctt
     1261 tcaggaagtg gacatcaccg gcatcacgct gcccattacc aaacacaact atctggtgac
     1321 gcgcgccgaa gacattgcac ctgcgctgcg cgaggccttt ctgattgcga agtcagggcg
     1381 gccaggtcct gtgctcgtgg acattaccaa agatgcgcag caagccacgg cggagtttaa
     1441 ctttgaggcc gcagcgccga agccatacag gccgcatccg atgcgcggcc atagtgccga
     1501 ttccatttcg gatgcggcac ggctgattcg tgaagcaaag cgcccggtca ttcttgcggg
     1561 gcacggcatc atccagtccg acgcatacga gcaggtacgc acgctggccg agcgcatgca
     1621 gattcctgtg gcttccacac tgctggggct gggcgcgttt ccggcatcgc atgagctttc
     1681 acttggcatg atgggcatgc atggcgaagc gtgggtgaac aacgccattc agaatgccga
     1741 cctgcttatc gcctgcggca tgcgctttga tgaccgcgtg acaggaacgc tggccacata
     1801 tgcgccgaat gctaagaaga tccacattga gattgatccg gcggagatca acaagaacgt
     1861 gaaagtagac gttgcgcttg tcggcgatct gaaacaggtg ctcgaggtcc tgctgccgca
     1921 gattccctcg aagtccagct cgccgtgggt acgcgagatc aatgagatga agggcgagtg
     1981 tgctgtccgc gacatcaaga acctgccgga caacggtcat ctctatgctg cgcatgtcat
     2041 caatgatctg tggaacgcga ccggcggcaa ggccattgtg gtgacggacg taggccagca
     2101 ccagatgtgg gaagcgcaat actataagca tgatgggccg cgcacgctgg tcacatccgg
     2161 cggactggga acaatgggct ttgcgctgcc tgcggccatc ggagcaaaag tggcctgccc
     2221 ggaaaaggaa gtctgggtga ttgccggaga cggcggcttc cagatgacgg ctgccgagct
     2281 ttcgaccatt gcgcaagaaa acttcgacat caacattgct gtaattaaca acggctatct
     2341 gggaatggta cgtcagtggc aggagttctt ctatgaaaag aactatgctt cctcgccgat
     2401 tctcagtccg gacttcgtga agctggccga tgcgcatggc attgcaggcg caacggtaac
     2461 gaaacgctct gaggtgctgc ctgccgtaga gtccgctcgc gaaaagaaag ggccgttcct
     2521 gattaacttc ctggtggaga aagaggactc ggtctatccg atgattccgc agggcaaggc
     2581 gctccacgag atgatccgga ggccagacaa tccgctgatt gaaaccgcgg aggatgcgtg
     2641 aaacagctgt tagcttccag ctttttagct agaggccagg agctatagca ttttcacaga
     2701 taggctgaac catgccgcga aactacagtc gccgcgacga acaggggtcg gcgacactgc
     2761 acagcgcaaa tggagtgtac agagtctgtg aaaatgccgt aggagcgaat tatgctgcat
     2821 acatttgttg cacttgtgga agacaagccg ggcgtactta cgcgcgtggc ctcacttttt
     2881 cgccgtctga atgtgaatat cgtttcactc accgtgggac gttcggagcg cgaaggcgtt
     2941 tcgcgcatga cgattgtcgc cgaggcatcg ccgacggcgg gacaccgcat tacggcaagc
     3001 ctctataagc ttgaaaacgt gctcgaagta gacgatgtgg gccagtttgc ctgcgtcacg
     3061 cgcgagttgg ctctgattaa agttgcggcg actccgcaga cgcgctcgca tatttttgaa
     3121 ctggtcgaag tcttccgcgc gcgcattgtg gacctggcga ctgagtcgct gatgatcgag
     3181 attaccggcg tctcaagcaa gattgaaggg ctgatccagg tactgatgga gagcggtgac
     3241 gaaattctgg aagtggcgcg caccggacgc atggtgatgc gtcgcggacg ccacaccagc
     3301 cgcgtaatgg a
//
LOCUS       DASRBV010000179        13597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_619509, whole genome shotgun sequence.
ACCESSION   DASRBV010000179 DASRBV010000000
VERSION     DASRBV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619509"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="prfB"
                     /locus_tag="VFA02_06770"
     CDS             complement(join(<1..1055,1057..1125))
                     /gene="prfB"
                     /locus_tag="VFA02_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265905.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZU09587.1"
                     /translation="MLNDLEFAYAPVRDKVRDLREYLDPNGLRKQLADIENKLSDPGV
                     WSNAAVSQPLMRERKRIEEQLAADDELARRTSDVEAYFDLAREGEQVEEDLAREIQVL
                     DEYASKLEERTMLSGETDPLNAIVTVHPGAGGTESQDWAEMLMRMYLRWAEQQGFKTE
                     INDYQDGEEAGIKSATFTISGEYAFGLLSGESGVHRLVRISPFDAAKRRHTSFASVFV
                     SPEIDDSIEVEVKPEDIRVDTYRSGGKGGQHVNTTDSAVRITHIPTGIVVQCQNERSQ
                     HKNREKAMKMLRSRLYEYELEKKKAETKKLEDSKLDINFGSQIRSYVLQPYRMAKDHR
                     TKVEVGDVDRVLDGYLEPFIRGYLLMKRNGGVPVAADAGD"
     gene            complement(1127..2749)
                     /gene="lnt"
                     /locus_tag="VFA02_06775"
     CDS             complement(1127..2749)
                     /gene="lnt"
                     /locus_tag="VFA02_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZU09588.1"
                     /translation="MTQRPVLLGLISGLLLDLPFPIAGPLPPWRAVFAWIAFVPLFYG
                     LLQECSVHSPRYLRRSALAGYACGVLWYILNCYWIYDTMHIYGNVPGPGAVGILVLYS
                     MVLGLYFALLGLLVALCRKAFRTNTVPLLLAPFFWAAIELAASRITSVPWDQLGYSQV
                     DNFLLTKLAPFSGVYGVSFALIAGNALLAAALLARSIHAKLRIGVGAVLLIVLLQVGS
                     FIQPKPELTSDYAVLLQPDLDVSVSNLWVGPEWDENAAHILELSQRHCTPFVMGMPSP
                     HAQMMYPQCPQDLPAPGLIAWPEAPSPFRSDDPRFAELMREIATKNHAPVVAGAIGRD
                     EAGEYNSAVFTAPDGNIIGRYDKIHLVPFGEFVPYRKVFFFAKHLTQQVGDFDRGKYR
                     KVFRANGHYFGVFICYESIFADEVRQFAIHGAQVFVNISDDGWYGDTSAPWQHLNMAR
                     MRAIENHRWVLRDTNNGVTTAIDPEGRVTYSALRHVNTSLAVRYGYENDLTFYSRYGD
                     VFAMLCGIISIGAIARAWRAVAFQSQRKVRSD"
     gene            2799..2924
                     /locus_tag="VFA02_06780"
     CDS             2799..2924
                     /locus_tag="VFA02_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09589.1"
                     /translation="MSALISFGMHLLTTLFFIGLCGSAIVIIISFIEDLQELFGE"
     gene            3037..5415
                     /locus_tag="VFA02_06785"
     CDS             3037..5415
                     /locus_tag="VFA02_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU09590.1"
                     /translation="MPVGQTFSAPQSNRVRLVVASSVMLTFISFWRAAAVVLNDLGSS
                     AFYAGGIAEEAIGKAAPWFILGVMLFSFAVRAVYVESCSMFTRGGVYRVVKEALGGTF
                     AKLSVSALMFDYILTGPISGVSAGQYITGLLNQLMQVGAAHGWLPPVLLSAAHTARQL
                     PMDGTSAVFCAAVTIYYWWQNIKGIEESSDKALEVMKITTIMVVLLLGWGFFTAFHLG
                     AKLPPLPVPSNLHFSKDALGFLGGTKFAASLGLFGILMAFGHSILAMSGEETLAQVNR
                     EIEHPKLKNLKRAAIVIAIYSFVFTGIVSLLAVMIIPDSVRVPVYRDNLIAGLAMYMV
                     GPLSLRIVFRVFVVIVGFLILSGAINTSIIGSTGVLMRISEDGVLTDWFRKPHRKFGT
                     SYRIVNLVTALQLFTIIASRGNVITLGEAYAFGVIWSFTFNSLAMLVLRWKYKGERGW
                     KVPVNIRIGKTELPLGLFCVFLTLLTIAVVNLFTKSVATESGIAFAAAFYAIFTVSEN
                     RNRKKHAATVKQMKEHFQLENQDTIGREALEIRPGGVLVTMRDAANPIALKWALSRTN
                     TDDQDIVVLTARMMGAGGPEYIDASDQLFSEHEQMLFTKAVSVAESFGKHISLLVVPA
                     GDIFAALVQTANSLEVSAVVSGLSTKMSAQEQAYHIGQAWEVLPEPKRQFTFYVVMPN
                     GEAQGFHIGPHAPTLQADDVQLVHRLWLNFRRDPEMQDLHHSDIVTYALTRLAVEYAR
                     DKQETLRDLRRYTSDGGKKAPLGPPYIRTSKESTDYPIPAPGPSSRQADSEK"
     gene            5930..6370
                     /locus_tag="VFA02_06790"
     CDS             5930..6370
                     /locus_tag="VFA02_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09591.1"
                     /translation="MCPSSRSIVLSSNLTKEHVIGFFRAQARGVLASDLTDFATLCKC
                     ITCVHKGQIWANSEQLGYLIESLSGSKALRIVDSRGEAILSAREEEVLHLLAEGLTNR
                     EMAASLHLSEHTIKNHLFHIFDKLGVSSRTEAILYAMSRRMVTR"
     gene            6822..12089
                     /locus_tag="VFA02_06795"
     CDS             6822..12089
                     /locus_tag="VFA02_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZU09592.1"
                     /translation="MSCLKPVWFLPVMALMLSGCGIHANPFAQLSLSPDTLSWHSVNV
                     GQTSSSKTVTVTNKSTSLAVSISAVNLSDGFVLTGNTCPTGTATLAPTASCTISVAFR
                     PVATGNQTGSLTIQDSAWGSPHSVSLSAKGGVGSLIFSPASLSFPGVPSGTISQPQNA
                     SLTNKQSSPITIQGFTVSGHFVEGTNCPISPSSLGAGESCTVTVASQPTAAGSITGSV
                     NAKDEFGNVTQLYLSGSDSGQPVTSPVSFSPASLTWGKVNVGQTSGSKTITVTNNQSS
                     SLAISYITTGPDFIQTGSTCPLAPSTVPAGGSCTISIAFRPATAGAKTELLSLTDNAS
                     GSPHSALLNATGTTGSLLFSPVSLSFAGVAPGNTSAPQQAMLMNQTSGAVVLASISVS
                     GHFAQTNNCPASLAPQTSCTFNVTSDPVANGTFTGSINVKDGSGNTTQLYLKGLGGTT
                     EQVTFAPDSLLWGKIAVGQTSGSKTVTLTNNQTTPLTISSISIGQDFILSATTCPTAP
                     TVVAAGASCSLSVSFRPLSSGIKNEDITIMDDAAGGVQNVSLQGTGVIGALLFNPSSL
                     SFAGVQPNTVSQPQTATLTNETASSDVTLASITVSGHFAQTNDCPGTLSAGQHCTITV
                     TSNPVTYGSTTGSINVKDGSNNTTQLYLSGMGGVPASTTSNGTNTPAASASPADSSSP
                     VYTTPTSLSWGTVAVGQTSGGKSITLNNSQTTPLTITSITSGQDFVQTANTCPISPNT
                     LAAGASCSVTMAFRPLSSGAKSEAVTFAYNGPGSPITVPLTATGTVGTLLYNPSSLTF
                     AAVAPGSTSPAQSSTLTNQTSAPVTLTSITVSGHFAQTNNCPISPSTLGAGASCTVNV
                     TANPVSSGTFTGSVNAKDSTGAVTQLYLTGSGTTSGGGGGSGSVTVSPKQYTFANQPV
                     GVASSPATITLTNGQSTALTVSSVQIASPFTQTNNCVGTVAAGASCSISVTYTPNAVG
                     YSSTNLTITDNAGNSPQTVAIAGNAYAPVKLSNSSFGFSNQIVGRSSTPQTLTLTNQQ
                     PTPLTINSITTSGSNEFQVSNTCIPNGSSTGTVPGNSSCTISVVFTPTTTGTVSGAFT
                     VSFNAWGSPATVTYTGTGIAGDTGTSVQIMPRSSCVLPSGQQQFAATVLHNSNTAVTW
                     YVDNVKNGNSTAGTISPSGLYTAPSVTGTHTIKAVSQAVSSAYASTSISVTSSPLFTI
                     YPYVASIPVNGQQPFQAQICNVPDNNVTFSVDNIQGGNATVGTIATDGLYTAPNTAGK
                     HIVRVTDPALNKTSGAVVNVFSGISVDFGSRTNTKFRVPAGILAVNHVDGLHNPTDMG
                     MIATMGTKVSRTYALIPQVYATQTPDWTKIDPLIANLQSLGMHILLQVAYTPTWLQPN
                     PNPCGAGNTTAAPTDVNKWAQIAASYVAHFDQKFPGVVTDYEIWNEPDTSGMCASNRL
                     NSYLALYAAAAPLMKQQAAADGVTIRVGGPTTAGVNSTWITALLTNPSTAPYVDFVSY
                     HNYVMSIQDIDATWDSYNGNNSVYQKTQEPGGGVASSYAKTAGLVAAGKQPLGAYTPI
                     YVDEYNTNWAFVQECCRNDPTYSPLWNAMYVSDLLNTVYSGTQAVPGVMAYYAAINYP
                     YFCLIGTWDTHMDCQYTTNSTPQPYPQYYAYQLMASPGYLDINSGGYMAASISPPSGG
                     GGFVTTAFYTPNQDSLLIVNPTATDYSSITINLQNTGYGSPHAVLYQIVGGQQITSQT
                     LALTQSNGTYTANIHVPPYTVLGIAVQGP"
     gene            complement(12207..13370)
                     /locus_tag="VFA02_06800"
     CDS             complement(12207..13370)
                     /locus_tag="VFA02_06800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09593.1"
                     /translation="MSFFLIALAWSVALVWAWRTEVALRNFSWLPDLLPETETLRPAA
                     RPFLSVIVPARNEEEAIEQTLRSLLAQQGVDLEILAVNDRSTDATGPIMEHVAASTDR
                     IRVLHIQELPEGWMGKTHAMAIAARQANAPWLLFTDADVVFREDSLRRALAYAEPEAD
                     HLVLFPTLVFKNFGECMMLSAIQMLTFLTWRPWKIADPKSRESIGIGAFNLVRRETYD
                     AIGGFEALPLEVLEDLRFGFLVKQHGFRQRFVLGRGLIRIHWAAGAFGIVRNLTKNIF
                     AVFRFNPLTLLGACIALAMFCFVPLIAIWGRWPARVSFLLVMMCAMAMYRCIVRRTTG
                     IRTAYVATFPVAACLILYALARSMLVTLARRGVVWRGTFYPLTELRKQAEPLR"
     gene            complement(13423..13593)
                     /locus_tag="VFA02_06805"
     CDS             complement(13423..13593)
                     /locus_tag="VFA02_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09594.1"
                     /translation="MFEPFFTTKPLGIGTGLGLDIANRVVTGHYEGHIGFTSEPGKTE
                     FIVKLPMSKVCV"
BASE COUNT         3147 a         4071 c         3353 g         3026 t
ORIGIN      
        1 tcatccccgg catccgctgc cacaggcact cccccattgc gcttcatcag aagatatccg
       61 cgaataaatg gctctaagta gccatcgagc acgcggtcta catcgccgac ttcgaccttc
      121 gttcgatggt ctttggccat gcggtagggt tgcagcacat acgagcggat ctgtgatcca
      181 aaattaatgt ccagctttga gtcttccagc tttttggttt ctgctttctt tttctcaagc
      241 tcgtattcat agagccggga gcgtagcatc ttcatcgcct tctcgcggtt cttgtgctgc
      301 gagcgctcgt tctggcattg aacgacgatg cccgtgggta tatgcgtaat gcgcacggcg
      361 gaatcagtgg tgttcacgtg ttgtccgccc ttgccgccgg agcgataggt atctacccgg
      421 atgtcttccg gcttgacctc gacttcgatc gagtcatcaa tttcaggcga gacaaagacc
      481 gaggcaaacg atgtatgcct ccgcttggct gcgtcaaaag gcgagatacg caccagacga
      541 tgtactccgc tttcacctga gagcagcccg aaggcgtatt cgccggatat ggtgaacgtc
      601 gccgacttga ttcccgcttc ttccccgtcc tgataatcgt tgatttccgt cttaaagccc
      661 tgctgctcgg cccagcgcag atacatacgc atcagcatct cggcccagtc ctggctttct
      721 gttccgcctg cgccaggatg cacagtcaca atggcattca gcgggtcagt ctcgccggaa
      781 agcatggtgc gttcttcgag cttgctcgcg tactcatcga ggacctggat ctcacgcgca
      841 aggtcttctt cgacctgctc tccctcgcgc gccaggtcga agtacgcctc gacatcgctg
      901 gtgcggcgcg caagttcgtc atcggcggca agctgctctt cgatgcgctt gcgttctcgc
      961 atcagtggct gagaaacggc ggcgttcgac cacactccgg ggtcggagag cttgttttca
     1021 atatctgcta gttgtttacg gagcccgttt gggtcaaaga tactcccgca ggtcgcgcac
     1081 tttgtcgcgc acgggagcgt aagcgaattc gagatcgttc agcaccttaa tcggacctga
     1141 ctttgcgttg agattgaaag gcaacggcac gccatgcgcg cgcaattgcg cctattgaaa
     1201 ttatcccaca gagcatggcg aacacatcgc cataacgcga atagaaggta aggtcattct
     1261 catatccata ccgcacagcc agcgacgtgt ttacatggcg gagcgcactg taagtcaccc
     1321 gcccttccgg atcaatggct gtagtcacgc cattattggt atctcgcagc acccagcgat
     1381 ggttctcaat cgcgcgcatc cgggccatgt tcaggtgctg ccaaggggcg ctggtgtcgc
     1441 cgtaccaccc gtcatcggag atattgacga acacctgcgc gccgtgaatg gcaaactgcc
     1501 gcacctcgtc agcaaaaatg gattcatagc agataaagac gccgaagtag tgcccattgg
     1561 cccggaagac cttgcgatac ttgcctcggt cgaagtcgcc aacctgctgc gtcaggtgct
     1621 tcgcaaagaa aaacaccttg cgatacggca caaattcgcc aaacggcacc aggtgaatct
     1681 tgtcgtaacg cccgatgata ttgccatccg gagcggtaaa caccgccgag ttgtactcgc
     1741 ccgcctcatc gcgtccaatg gcgcccgcaa ctaccggagc atggttcttt gtcgcaattt
     1801 cccgcatcag ctcggcaaag cgcggatcat cgctgcgaaa gggcgagggg gcctccggcc
     1861 acgcgatcag gccaggagca ggcaagtcct gcgggcattg cggatacatc atctgcgcat
     1921 gaggcgaagg catgcccatg acgaacggcg tgcaatgccg ctggcttaat tcaagaatat
     1981 gtgcggcatt ctcatcccac tcagggccaa cccagagatt ggacacgctc acatcgaggt
     2041 ccggctgaag cagcacagca taatccgagg tcagctccgg ctttggctgg atgaaagacc
     2101 ccacctgcaa cagaacaatc agcagcaccg cgcccacgcc aatccgtagc tttgcatgta
     2161 tggagcgggc cagtagcgcc gcggccagca aggcattacc cgctatcagc gcaaaggaaa
     2221 ctccataaac cccgctaaac ggggccagct ttgtaagtag aaaattgtcc acctgcgaat
     2281 aaccaagctg gtcccatgga acgctggtaa tgcgcgacgc ggccagctca atcgccgccc
     2341 agaaaaacgg cgccagcagc agcggaactg tgtttgttct gaatgccttg cggcaaagcg
     2401 cgaccagcag accaagcaac gcaaaatata gccccagcac catgctgtag agcaccagaa
     2461 tccctacggc gccgggcccg ggcacattgc cgtaaatgtg catggtgtcg taaatccagt
     2521 agcaattcag gatgtaccag aggactccgc aggcgtatcc cgcgagagca ctgcggcgca
     2581 gatatcgcgg ggaatgcacg ctacactcct gtagcagccc atagaacaac ggaacaaaag
     2641 caatccaggc gaagacggca cgccagggcg gcagcggccc cgcgatggga aacggtaaat
     2701 ccagtagaag tcctgaaatc agccccagga ggacggggcg ctgggtcaaa aaacgcatcc
     2761 gaggctgagt ctaccatccc ggcgttacaa tacttgctgt gagtgccctc atttccttcg
     2821 gaatgcacct gttgaccacc ctctttttca tcggcctttg cgggtcggcg attgtcatta
     2881 tcattagttt tattgaggac ctgcaggagc ttttcggcga atgagcagcg cgcatatttg
     2941 actgtgtttc ggtctggaca tacactcagc gctgagcgtc cttgccgagg cagatgttcg
     3001 ccctgcggtg ggtagcaagc gattcaccca taaaccatgc ctgttggtca aacgttttcc
     3061 gctccacaat cgaaccgggt acggcttgta gttgcctcct cggtcatgct caccttcatt
     3121 tctttctggc gggccgcggc cgtcgtcctc aatgacctcg gttcatcggc cttctatgcc
     3181 ggaggtatcg ccgaagaggc cataggaaaa gccgcgccgt ggttcatcct gggcgttatg
     3241 ctcttttcct tcgccgtacg cgccgtttac gtcgaaagct gttcgatgtt tacacgcggc
     3301 ggcgtctacc gcgttgtaaa agaggcgctc ggcgggacat ttgccaaact cagcgtctcg
     3361 gccctgatgt ttgactacat ccttaccggg cctatctccg gtgtgtccgc aggccagtac
     3421 atcaccgggc tgttaaatca actgatgcag gtgggcgcag cgcatggatg gctgccccct
     3481 gtcctgctga gtgctgctca cactgcccgg caattgccca tggacggcac ctccgccgtt
     3541 ttctgcgccg ctgtcaccat ctattattgg tggcagaaca tcaagggcat tgaagaatcg
     3601 agcgacaagg cccttgaagt catgaagatc accaccatca tggtggtcct cctgctggga
     3661 tggggtttct ttaccgcctt tcatcttggc gccaagctgc cgccgctgcc tgtcccatcc
     3721 aacctgcact tctccaaaga tgctctcggt tttcttggcg gaacaaagtt tgcagcgtca
     3781 ctgggcctgt tcggcatcct gatggccttc ggccactcca tcctggccat gagcggtgaa
     3841 gaaacactgg cccaggtcaa ccgggagatt gagcatccga agctcaaaaa ccttaaacgc
     3901 gccgccattg tcatcgccat ttacagcttc gtctttacgg gaatcgtttc gctgcttgcg
     3961 gtgatgatta ttcccgactc ggtgcgcgtt ccggtttacc gtgacaacct gattgcagga
     4021 ctcgccatgt atatggtcgg tccactttcc ctgcgcattg tcttccgcgt ttttgtggtc
     4081 attgtcggct tcctgatcct ctccggcgcc atcaatacct ccatcattgg ttcaaccggc
     4141 gtcctgatgc gcatctcgga agatggcgtg ctgaccgact ggttccgcaa gccacaccgc
     4201 aagtttggca ccagctatcg catcgtcaat ctggtgaccg ccctgcagct tttcaccatc
     4261 attgccagcc gcggaaacgt catcacgctg ggtgaggcat acgcttttgg cgtgatttgg
     4321 agcttcactt tcaactcgct ggccatgctc gtcctgcgct ggaaatataa aggcgagcgc
     4381 ggctggaaag tgcctgtaaa cattcgcatc ggtaagacgg aacttcccct cggtttgttc
     4441 tgcgtctttc tgaccctgct gaccatcgca gtcgtcaacc tgtttacaaa gagtgttgcc
     4501 actgagagcg gcatcgcctt tgcggctgct ttttacgcca tcttcactgt ttcggaaaac
     4561 cggaacagga aaaaacatgc ggccaccgtc aagcagatga aggaacactt ccagcttgag
     4621 aaccaggaca cgattggccg cgaagccctg gagatccgtc ccggcggagt gctcgtcacc
     4681 atgcgtgatg cggcaaatcc tatcgcactg aagtgggcgc tctcccgcac aaacactgac
     4741 gaccaggaca tcgttgttct caccgcccgc atgatgggcg ctggcggccc ggaatacatt
     4801 gacgcttccg accagctatt cagcgaacac gagcagatgc tctttaccaa agctgtttct
     4861 gtcgcagaaa gctttggcaa gcacatttcc cttctggtcg ttcccgccgg agacatcttc
     4921 gccgcactgg tgcagaccgc aaactcgctt gaagtctcag ccgtagtctc tggtctgtcc
     4981 acaaaaatgt ctgcccagga gcaggcctat cacattgggc aggcatggga ggtccttccg
     5041 gaaccgaaac ggcagttcac gttctatgtc gtcatgccca atggagaagc gcagggcttc
     5101 cacattgggc cgcacgctcc aacattgcag gccgatgacg tgcagctcgt ccatcgttta
     5161 tggctgaatt tccgccgcga cccagaaatg caagacctgc atcacagcga cattgtgact
     5221 tacgccctca cccgcctcgc cgtcgagtac gcgcgcgata agcaggaaac attacgcgat
     5281 ctgcggcgtt ataccagcga cggcgggaaa aaagccccac tcgggccgcc ttacatacgc
     5341 accagcaaag agagcacgga ctatcccatt cctgcgcccg ggccttcctc gcgccaggct
     5401 gacagtgaaa aataaaccag taaagtggct taggacccaa atttgaacac gtaatcgctt
     5461 ggcggctgcc gggtaaccat gtttttgcgc tgcacgatta ccctaagtta tagagcaggt
     5521 tgttactttt ttgatgaggc attttcaggc aattccactg taaactcttt gcttagtaag
     5581 aatgtcgcat ttcagtatgt gatattaagc ctggtacaaa cttagaggca cgtgcaggta
     5641 tttcttgttt tcagagcagt tcctaagtgg catctgaagg gtttctatga gcactttttc
     5701 cacccagtca cggatatcta tcgttattgt tgattcaacc agaatcacat ctgaactttt
     5761 ggaacaggca ttttcgaagc accctcagtt tgtcatcctt ggctgcataa gaaatctgga
     5821 cgacctgaca agacttcttt ctcagaagca acctgatatt gttcttattc agtgctctga
     5881 taaaaaagac cagcctaatg ccattgctgt cctggataag gtatctgcaa tgtgtccgtc
     5941 ttcgcgttcc atcgtacttt cttccaatct gacgaaagaa catgttatcg ggttctttcg
     6001 cgcacaggcc cgtggtgttc tcgcctcgga ccttacagat tttgcaaccc tctgcaagtg
     6061 tattacctgt gttcacaaag ggcagatttg ggcaaacagc gaacagttag gatatctcat
     6121 cgaatcgctc tccggatcaa aggccctcag gatcgttgat tccagaggag aggcaatttt
     6181 aagcgcgaga gaagaagagg tcctgcactt gctggctgaa ggtcttacga accgcgaaat
     6241 ggctgcatct ctgcatctca gcgaacatac gataaagaac cacttgttcc acatctttga
     6301 caagctcggc gtctccagcc ggacagaagc catcctctac gctatgagcc gtcgcatggt
     6361 cacacgatag agtgcaccat aacttcactg catccgtgat tgctgagtgc tcataaagcc
     6421 gcaggcttgt atgggatagt tctgtcccgc ccgcgaatat cttctcatct aactcatact
     6481 tatttctcat tgcgtaagag tatagtaacc gcctgttttc attactaaat aactgatgct
     6541 tatggaagtt attggatagt acgataagtc tgattttgtc tattaaggct tgattaaaaa
     6601 atatgcgacg cctttcgccc atttcatcta gttctaataa cttttgcttt tatgggcgaa
     6661 gcatctcccg gcacgcggtt gccagcaaac aacctgttta tcttggccct gcgcctgttt
     6721 tctttcaagt ccagtgaatg gaagaggtcc ctatgcgacg ctatcaaccc acacgttccg
     6781 ctagccaact cgttctgtcc agatggttcc agcataaaag catgagttgc ctgaaaccag
     6841 tatggtttct acctgtaatg gccctcatgc tcagtggatg cggtattcat gcaaacccat
     6901 ttgcccaact gtcgctttca ccggacaccc tttcgtggca cagtgtcaat gtaggccaga
     6961 cttcttcctc gaaaactgtc accgtcacaa acaagagcac ctctctggct gtatctattt
     7021 ctgcggtcaa tctaagtgat ggatttgtcc ttaccggcaa tacctgcccg acaggaactg
     7081 ctacactcgc ccccaccgct tcctgcacca tctccgtcgc gttccgtcct gttgcaacag
     7141 gaaaccagac cggatcactc accattcaag acagcgcctg gggaagcccg cattccgtat
     7201 cattgagcgc aaaaggaggc gttggatcgc tgatcttttc gcctgcgtct ctgtcgtttc
     7261 ccggagtacc ttccgggacc atcagccagc cgcaaaatgc ctcactaacc aataagcaat
     7321 ccagtccaat tacgattcaa ggctttacag taagtggaca ctttgttgaa ggtactaact
     7381 gtccgataag tccgagcagc ctcggcgctg gagaaagctg tacagtgact gtggcttctc
     7441 aacccacagc agcgggaagt atcacaggat cagtcaatgc taaagatgag tttggaaatg
     7501 taacgcagtt gtatctttcc ggctcagaca gcgggcaacc agtaacatca ccagtcagct
     7561 tctctcccgc ttcactgacg tggggcaagg tcaatgttgg tcagacttcc ggctcaaaga
     7621 ccatcacggt gactaacaat caaagctcat cgctcgcaat ttcttacatt acgacaggcc
     7681 cggatttcat tcagaccgga agcacctgtc cgctcgcgcc ttctaccgtg cctgccggcg
     7741 gctcctgcac aatctccatc gctttccgtc cagctacagc aggagcaaaa acagaattgc
     7801 tctcgcttac agataatgct tccggcagcc cgcactctgc gcttctgaat gcaacaggca
     7861 ctaccggatc gctgctgttc tctccagttt ctctgtcctt cgcaggagtt gctcctggaa
     7921 ataccagcgc accacaacag gccatgctca tgaaccagac atccggagcg gtcgtgctcg
     7981 ccagcatctc tgtcagcggg catttcgcac agacaaataa ctgcccggcc agcctggctc
     8041 cgcaaacaag ctgcaccttt aacgtgacat cggaccctgt cgcaaacgga acatttaccg
     8101 gctcaatcaa tgtaaaagac ggctctggaa atacaaccca actttatctg aaaggcctgg
     8161 gcggcaccac tgaacaggtg acctttgcac cagactcact gctgtggggc aaaatcgctg
     8221 tcggccagac gtccggttca aagactgtta ctctgacgaa caatcagaca acgcctctga
     8281 cgatttcttc tatctccatc ggacaggact tcattctttc agcaactact tgccccactg
     8341 ctccaaccgt cgtcgctgcc ggggcttcct gctcattgag cgtgtccttc cgtccgctct
     8401 cgtctggaat caaaaacgaa gacatcacca tcatggatga tgccgctggc ggcgtgcaga
     8461 acgtttcttt gcaaggcaca ggcgtgattg gagcgctgct ctttaatccg tcttctctct
     8521 cgtttgcggg agtgcagccg aacacagtca gccagccgca aaccgccacg ctgaccaatg
     8581 agacagcgtc ctctgatgtc actttggcga gcatcaccgt cagcggccat tttgcgcaga
     8641 cgaatgattg ccccggtacg ctctccgccg gccagcattg caccattacc gtaacttcca
     8701 acccagtcac gtatggaagt acaactgggt ccatcaatgt aaaggatggc tcaaacaaca
     8761 caactcagct ctatctgagc ggcatgggtg gagttcccgc aagcacgacc agcaatggga
     8821 ccaatacgcc tgctgcttcc gcttccccgg ccgacagcag cagtcctgtc tacactacgc
     8881 caacaagcct gtcgtggggc accgtcgcag taggacaaac ctccggcggc aagagcatta
     8941 cgctcaataa tagtcagacc acgccgctga ccattacatc cattacctca ggacaggact
     9001 tcgtgcagac ggcgaacacc tgcccgatct cgcccaacac tctggccgcc ggagcaagct
     9061 gctctgtaac catggcgttc cgtccacttt caagcggcgc caagagtgaa gcagtcacgt
     9121 ttgcatacaa cggcccaggc agtccgatta ccgtgcctct caccgctacc ggaacagttg
     9181 gcactttgct ctacaacccg tcttcgctga cattcgccgc cgttgctccg ggctcaacca
     9241 gtcccgcgca aagctcgacg cttaccaatc agacctccgc tccggtcaca ctcaccagca
     9301 tcactgtgag cggacatttc gcgcagacca acaattgccc catcagtccg agcacgcttg
     9361 gagcaggcgc gagttgcaca gtcaacgtga ccgccaatcc tgtttccagc ggaacattca
     9421 ctggctcagt caatgcgaaa gacagcaccg gtgcggtcac gcaactctat ctcaccggtt
     9481 cgggcacaac ttccggaggc ggtggcggaa gtggcagcgt cacggtctcg ccaaagcaat
     9541 acacttttgc caatcagccg gttggtgttg caagctcgcc cgcaaccata acgctcacaa
     9601 acggccagtc tactgcgctt acagtctctt ccgtgcagat tgcgtcgccg ttcactcaaa
     9661 ccaacaattg cgttgggacc gtagcagcag gtgcgagctg cagcatttcc gttacgtaca
     9721 ctcccaacgc tgtcggctac agcagcacaa atctcacaat tactgacaat gccggcaatt
     9781 ctccgcaaac ggttgcgatc gctggcaatg cctacgctcc cgtcaagctg agcaacagca
     9841 gtttcggttt cagcaatcag attgtcggcc gctccagcac gccgcaaacg ctcacgctga
     9901 cgaaccagca gcctacgccg ctgacaatca actccatcac tacatcgggc agcaatgaat
     9961 tccaggtcag caacacgtgc atcccgaacg gaagcagcac cggtactgta cccggaaact
    10021 caagctgcac catcagcgta gtattcactc caacaaccac cggaacggtc tccggcgcgt
    10081 tcaccgtttc cttcaatgca tggggcagcc ctgcaaccgt tacttataca ggcaccggca
    10141 ttgcaggaga tacgggaacc agcgtgcaga tcatgcccag atcatcctgt gttctaccct
    10201 cagggcagca gcagttcgcg gccacagtcc tccacaattc caacactgcc gtcacgtggt
    10261 acgtggacaa cgtcaagaac ggaaacagca ctgccggcac catttcacct tccggactct
    10321 acacggcgcc ctcggtcacc ggaacacaca ccatcaaggc tgtgagtcag gcagtctcat
    10381 cggcctatgc ttccacctcc atcagcgtga cctccagtcc gctcttcacc atttatccat
    10441 acgttgcctc cattcctgtc aatggccagc agccgttcca ggcacagata tgcaacgttc
    10501 ctgataacaa cgttactttc tcggtggaca acatccaggg cggcaatgca actgtgggaa
    10561 caatcgctac tgatggtctg tacaccgctc cgaataccgc cggcaagcac atcgtcaggg
    10621 tcacagatcc agcactaaac aagaccagcg gcgcggtcgt gaatgtcttc tcaggtatct
    10681 ctgttgattt cggatcacgc acaaacacca agttccgggt cccagcaggc attctcgcag
    10741 tcaatcacgt ggatggtctg cacaacccaa ccgatatggg catgattgca accatgggca
    10801 caaaggtgtc acgcacctac gcgttgattc ctcaggtcta tgcgacgcaa acacccgact
    10861 ggacaaagat tgatcccctg attgccaacc tgcaatctct gggcatgcac atcctccttc
    10921 aggttgccta tactccaacc tggttacagc cgaaccccaa tccctgcggc gctggaaata
    10981 caacagcggc tccaactgat gtgaacaagt gggcacagat tgctgcttcc tacgttgctc
    11041 atttcgacca gaagttcccg ggtgttgtta cagattatga aatctggaac gaaccggata
    11101 catcgggcat gtgcgcttcc aaccgattga atagctatct cgcgttgtat gccgccgccg
    11161 ctccgctcat gaaacagcag gctgccgcag atggcgtcac catccgcgtt ggagggccaa
    11221 cgacagcagg cgtgaactca acatggatca ctgccctgct caccaacccc tccaccgcac
    11281 catatgtaga ctttgtgagt tatcacaact acgtcatgtc cattcaggac atagatgcga
    11341 cctgggacag ctataacggc aacaactcgg tctaccaaaa gacccaggag ccaggcggtg
    11401 gcgtagcatc ctcgtatgca aaaacagccg gcctggttgc ggcaggaaag caaccgctcg
    11461 gcgcatacac gcccatctac gtagatgaat acaacacaaa ctgggcgttt gttcaggaat
    11521 gctgccggaa tgacccgacg tattctccgt tgtggaatgc gatgtacgtc agcgatctgc
    11581 tgaatacggt ctactcaggc acgcaggccg ttcccggagt catggcatac tacgcggcca
    11641 tcaattaccc ctatttctgc ctcatcggaa catgggacac ccatatggat tgccagtaca
    11701 caacgaacag tacgccgcaa ccttatccgc agtactatgc ctaccagtta atggcctcgc
    11761 ccggttatct ggacataaac tccggcggat acatggctgc ctccatctca ccgccatccg
    11821 gcggaggtgg cttcgttacc accgcattct ataccccgaa ccaggactca ctgctgattg
    11881 tgaatcctac agctacggac tactcatcca tcaccatcaa cctgcagaac acaggctatg
    11941 gttcgcccca tgctgtgctg taccagatcg tcggcggaca acaaatcacc agccagacgc
    12001 tggcgctgac acagtcgaat gggacctata cagccaacat ccacgtaccg ccttacacag
    12061 tacttggcat cgcggtccag gggccttagt ttctaaatcc tctgtttggg atttatttaa
    12121 gcggtagcat tgcggaacct gctaccgctt ctttttttga atattgcctg gcctcattgt
    12181 tcccttgcgt ctgcggttaa gtctctttac cgcaatggct cggcctgctt ccgtaactcc
    12241 gtcagcggat aaaacgtgcc tcgccagaca acacctctgc gagcaagcgt tacgagcatc
    12301 gagcgggcca gagcgtaaag tatcaggcat gcagccaccg gaaaagtagc aacgtatgcc
    12361 gtgcgaatgc ccgtcgtccg gcgtacgata cagcgataca tggccatagc gcacatcatt
    12421 accagcagaa aggaaacacg tgccggccac cgtccccaaa tcgcgatgag aggaacaaag
    12481 cagaacatgg ccagcgcaat acaggcgccc aacagcgtga gcggattgaa ccgaaacaca
    12541 gcaaagatgt tttttgttag attgcggact ataccgaacg cgcccgcggc ccagtgtatg
    12601 cgtatcagac cgcgtccaag cacaaagcgc tgacggaaac catgctgctt cacaagaaac
    12661 ccgaagcgca ggtcctccag cacttccagc ggcagcgcct caaagccgcc gatagcatca
    12721 taagtttcgc gccgcaccag gttgaatgct cctatgccga tggattcgcg gcttttcgga
    12781 tcagcaatct tccacggacg ccaggtaaga aacgtcagca tctggattgc ggagagcatc
    12841 atgcattctc cgaaattctt aaagaccagt gtcgggaaca gcaccagatg gtctgcttca
    12901 ggttccgcgt aagccagcgc gcgccgcaag ctgtcctcgc gaaagaccac atcagcatcg
    12961 gtaaacaaaa gccatggagc gttggcctgc cttgccgcaa ttgccatcgc atgggtcttg
    13021 cccatccagc cttcaggcag ctcctgaatg tggagaacac ggatacgatc tgtgctcgca
    13081 gcaacgtgct ccataatggg gcctgttgcg tcggttgagc ggtcatttac tgcaagaatc
    13141 tccaggtcca cgccttgctg cgccagcaga gaccgaagtg tctgctcaat tgcctcttct
    13201 tcattgcgcg caggaacaat cacagagagg aatggtcttg cagcaggccg gagtgtctca
    13261 gtctccggca aaaggtccgg cagccatgaa aagttgcgca gagcaacttc agtgcgccag
    13321 gcccatacaa gcgcaacgga ccatgcaagt gcaattagga agaaggacac gctcttagta
    13381 tggcagagca aaactgtaca aagattctgc cagcagcgta acttacacgc agactttgct
    13441 catcggcagc ttcacgatga actcggtctt gccaggttcg ctggtaaaac caatgtggcc
    13501 ttcataatga cctgtaacaa cccggttggc gatgtccagt cccaggcccg tccctatgcc
    13561 aagcggcttg gttgtaaaaa acggctcaaa caccttg
//
LOCUS       DASRBV010000180        29010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_365068, whole genome shotgun sequence.
ACCESSION   DASRBV010000180 DASRBV010000000
VERSION     DASRBV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 29010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29010
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365068"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFA02_06810"
     CDS             <1..667
                     /locus_tag="VFA02_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU09595.1"
                     /translation="RENLQQMIVVTGRLEGSDLGTAMAQVQRAVENLHLPSNVRVTYG
                     GTYEEQQKSFHDLMRVLLLALALVFGVLLTEFRNFAAPVAILTSSILSISGVVIALLI
                     TGTTFNVASFMGLIMVIGIVAKNGILLLDADEKFRAEGASPRDAMVHAAQRRLRPILM
                     TAIAAVCGMLPLAFAIGSGSQMLQPLAIAVIGGLCISMVLSLIVTPVVYYFLTRRREQ
                     QIF"
     gene            complement(714..2129)
                     /locus_tag="VFA02_06815"
     CDS             complement(714..2129)
                     /locus_tag="VFA02_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09596.1"
                     /translation="MKPYSITRRLIVTVLLVELIAALCVTGMAWFYERHSHFRAFDIM
                     LRGRADSLLGSVEDADDAEDNVMLDMKDLAVPREDIYEVRDEKGHLLGRSPNWEGASA
                     QMLTSQQDRMFPLRLHGRSYRVLLRHGLRIVDPNDASGGVPHHVTVVYGSPTKHVWHA
                     VIDTVQFYAISSLALLAVTGAVMAWLLNRSLAPLHELAAEASTVSVNSWKFTPSERAC
                     ATKELFPLVQALEAALQRLERSFMQQRRFVSDSAHELKTGVAVVKSSLQLLGMKPRSA
                     EEYQAGLARCEADCERMEEIVAKMLTLARVDASAETHAPSVTDVGQALQQVQEQLQPI
                     ADTRGITITRIVPEALSAHLAPQECSLLFSNLLLNALQHSVSGTTVSIQGHACNGYAE
                     IQITDQGDGIPPEALPHVFEPFYRGDASRNRNTGGTGLGLSICKAIVDKAGGDIRITS
                     QPGKGTTVTVQLPGLHETSES"
     gene            complement(2126..2800)
                     /locus_tag="VFA02_06820"
     CDS             complement(2126..2800)
                     /locus_tag="VFA02_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09597.1"
                     /translation="MRVLVVEDEPRIAENVAAALRDGPGFAVDIAEDGEVGFFLAKDT
                     CYDLIVLDLMLPKLDGFSLLKKLRSSNSTTPVLVLTARDETRSIIDLLNAGADDYLSK
                     PFDLGELIARAKALIRRGKGAAHPVLTVADIEVNTLEQSVRRAGEIVDLSPMEYRILE
                     YLIHRPRVVVSKRELLEHLYDYNWEHHSNVIEAHVSNLRKKLDAGSSQPSIETLRGRG
                     YRLAIA"
     gene            2842..3525
                     /locus_tag="VFA02_06825"
     CDS             2842..3525
                     /locus_tag="VFA02_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZU09598.1"
                     /translation="MAVEQVFQHLKWMFMFYDRRDAGRRLAKRLQEYARREDVVVAGL
                     LRGGIPVAFEIAQKQHVPLDVLLVRKLGAPNQPELAMGAVAPGGTRILDHSLIRKLGL
                     SEQQVDEMISAEEEKLHRGEQLYADVRPQITVRDKTLIAVDDGIATGASMMAGVSVLR
                     AQGAKKVVVAVPVAPPHAVRELSKVADEVVSLKITDYFPAVGFFYRDFQQTTDEEVRD
                     LLRRASQHI"
     gene            complement(3513..4943)
                     /locus_tag="VFA02_06830"
     CDS             complement(3513..4943)
                     /locus_tag="VFA02_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HZU09599.1"
                     /translation="MSSTTHINIGLCGIGLEAYWDQFAGLKQRLEANIQTVAKRLAQH
                     DVHITNLGMIDSTGRAREAGHAFRRAEIEVLFLYTTTYALSSTVLPLVKRIGVPVIVL
                     NLQPGPAIHYATFNKLNDRTAMTGEWLAWCSACPVPEIANVFARAGIQFHEAIGVLGD
                     EQTWSEIEDWLAAAKVKSQLENNRLGLLGHYYSGMLDIMTDVTQICITFGGDVEVLEV
                     DELSALREQVPDAEITRRISEFQQHFDIQPDCSDEELRRAARTSVALDTFVAKHRLTS
                     LACYYKGTGVPANEDTMSSIILGTSLLTSRHIPVAGEYEVKNAIAMKIMDALNVGGSF
                     TEYYALDYAADVVLMGHDGPGHIAIAEGKTKVKPLQVYHGKVGRGLSVEMSVKHGPVT
                     LLSIVEDPGHSFKLIAAEGESVSGSILEIGNTNSRYRFPVGAKQFTAKWNAQGPAHHC
                     AVGVGHIAHTLEKLARLLGIPFHQIC"
     gene            complement(5001..5300)
                     /locus_tag="VFA02_06835"
     CDS             complement(5001..5300)
                     /locus_tag="VFA02_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HZU09600.1"
                     /translation="MLKLRPGAGPAYDKAHEAVWPEMLALLKNAGVHEYSIFRRDEIL
                     VLTMRVDDFEQTWQRIEQSPVNTRWQQAMAEYFAPLEGLRSGERFPMMREVFYLP"
     gene            complement(5330..6043)
                     /locus_tag="VFA02_06840"
     CDS             complement(5330..6043)
                     /locus_tag="VFA02_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09601.1"
                     /translation="MTRPKFHAKVFLFQWIGNLLLILLCFAWLQIPDSHVWQFVFSML
                     SAAILVVAFLWLYSHTFRLLYRAKAPAPIWQRWLALVVVIVVGYLLLLGLDALRSHES
                     LYAGYWNSKFSAGMRAFFTYPRLLSWQDHFYDFLQWTFAALLLPVALIGSASGLRHGR
                     WKNIGRVFNKVLYWVYALAAAFIGTWISSLLVGWTPTGKPSAEIISVILRIGVAYTID
                     VLLWCLVLGVIAEYLQKSE"
     gene            complement(6040..10758)
                     /locus_tag="VFA02_06845"
     CDS             complement(6040..10758)
                     /locus_tag="VFA02_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZU09602.1"
                     /translation="MEHSARRKLSSTRFVLSLFFLLASIAASQAIHAERAVSFNLSTS
                     RTFSPDEKPSIHLYTHNVDELEFRVYHINDPEKFLSNLRDLHSFENGTPWAPKEQIDE
                     RTWLERFHDWKHHLWFLVRQFFRRQFSYETRDFLRAKQSSLAHRSRIVGVAQFAQIPL
                     LNSQQLIARWRQEVPPTYISDSQDLPIDPLPSGFYLVEATDGHYKAYTVLMVSRVALV
                     TRTGSGQVLAYVVDRHSGAPIPHAQVKLGFGPHEQASAETGPDGLAELHGPVSQQERD
                     NIWVIATADKDVAAVTPAGYAFSGTQSNRWASFVYTDRPVYRPGHTVHWKAILRERIE
                     NHLELPKISSIHAVVSDQEDHSIFEKDLPISKDGTVTGDLTLANNAMLGYYSIRLGDG
                     NDTAGGSFRVEEYKKPEYQVRVVPAKPRVLQGETNQVVIDSRYFFGEPVANAEVTYRI
                     YHSPHYWWDEGEPDDTAGGPPAVGEDEQQPDLYDSEQESEQTGKLDANGKLTISVPTK
                     FDSSGKQRSDQDYTIEAGVTDAANREITGRGRFLATYGSFRIHVEPTSYSVRAGEAAN
                     FRLTAIDYDNKPVQTQIHLQLVYRRWVNGKTETTQGSSTDVTTDAQGQATASLPAQNA
                     GSAEVEASANTPEKRTVQDSTWLWVMGNGESPWYGSSQTVQIVADKKTYAPGDTAHLS
                     IISQVDNFHALVVASGYSVEFKKVLSSEGKTLSFDLPITSDSQPNLDVSAVYIQNDQM
                     YQASKQLKVPPVQEQLQIEIAPASQVFQPQQSATYDVYTRDYQGKPVSADLSFGVVDE
                     ALYYLYPDTSGDIVKRLYPDRYVYAPFDSSLQYYFSGSAGTKSPMLAERQSRYRPQLA
                     QVKPGNDVVQPKVRKAFPDTAYWAANVHTDTQGHARVSFTFPDSLTTWRTTVRAVTPD
                     SKAGAAINRVLVRKNIIVRMGAPRFMRKGDELTIPVIVHNYLDQAKQIQLSLDIHGLD
                     TIAGTPQTITVPSKADGTVLWRVRASQIGTATLLAKALSTQESDALEISFPVEPSGVV
                     QKLNQLGVVEDANGQVAASINFPANTDPAAHKLHIEVSPSIAGSIFSALDYLTSYPYG
                     CTEQTMSSFLPDVIIAETLKKLNLPSRVDLNDLELKVRAGLDRLADFQHDDGGWGWWK
                     EDDSRVFMTAYVISGVARAKQAYPLDDVKQQMFGKGVEYLQKAVAEHPRMLPELRAYV
                     VYALTEADAGNTGDSLNTLWSRRNDLSAEGLALTGLAMLHTSDSRASQIAKLLEDKAK
                     RSGALAYWPSDYNPLLDLYYDNNAESTAFAMRFLAHTDPKNPLLQQAAQWLVLNRNGG
                     YWWNSTEQTAMVIFGLADYLATSQELNADFDVDVLVNGHSVAKRHFNANDATSGASLQ
                     VDIPATNLQPQGNSIQIVKHGTGRAYWSAQGIYYSTETKLYQKGNLSLNLTRDYFKLV
                     PTQKKDQIVYQLQPLKGPVSPGDVLAVHIAANGSPVKYLMIEDPIPAGTEFIQNSDSY
                     NIVGQPDTWQFWYTRQEFHDDRAAMFATEFDGRHESFYLLKVVNPGSFSISPARVELM
                     YQPGIEATSDMLHLDVEAKQ"
     gene            complement(10731..11774)
                     /locus_tag="VFA02_06850"
     CDS             complement(10731..11774)
                     /locus_tag="VFA02_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HZU09603.1"
                     /translation="MDTLAKVKGRSGKAATLVILTAAVVATVFLGLLHQERARTPIIL
                     PREVAMPADGRFHSAFLIRLANGGPLSTAEFRYNAENLRLLQKNRGTIEGQLRSPVLS
                     GQQKIHLDWKKKPLTVHAQFLPDYTDSFSDGTPDFLRLHTEEDRHAFRAWFTALAEFE
                     AAKAKDALPAEINDCAALLRYAYRNALHAHDPGWLVGEHMEELAGLPSIQQYHYPDTP
                     LGASLFRVKSGPFLPEDLSNGSFSQFADAKSLMLFNTHFISRDVRMAQPGDLIFFRQL
                     EQNSPYHSMVVTGSHSDWVVYHTGPIGNAKGEVRRVAIEDLLHHPDARWRPVSENSNF
                     LGVYRWNILRDGN"
     gene            12162..12974
                     /gene="pgeF"
                     /locus_tag="VFA02_06855"
     CDS             12162..12974
                     /gene="pgeF"
                     /locus_tag="VFA02_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZU09604.1"
                     /translation="MGGKTTVYSGEHKSGELNLGFTPADSRETVLANRETFIRDLTEG
                     RATLPAFVTLKQMHSSIIHVVGKADAGEEAVLTGDGMLTGEAGVLLGIQTADCIPVLV
                     ADKKQRAVAAFHAGWRGTLKRIVENGIGRMRLEFGSKPEDLSAAIGPGIGQCCYAVGE
                     EVRDAFHSQFAYAQDLFREVYNADPIKEKYPMLFLTARAPGHSNLGPNLHLDLVEANR
                     RQLLDAGLREEQIFVLGDCTSCHVDRYFSYRAEHGFTGRMLSVIGVRDRSLS"
     gene            complement(13215..13523)
                     /locus_tag="VFA02_06860"
     CDS             complement(13215..13523)
                     /locus_tag="VFA02_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HZU09605.1"
                     /translation="MTKADLVEEVTQLGDLTRRDGEVIVDTIFDSVIQALKAGDKIEI
                     RGFGSFRIRQRKPRTGRNPKTGAKVEVPAKRVPYFKPSKELRDMVNPKSEGGSPEQPA
                     "
     gene            complement(13589..14497)
                     /gene="sppA"
                     /locus_tag="VFA02_06865"
     CDS             complement(13589..14497)
                     /gene="sppA"
                     /locus_tag="VFA02_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZU09606.1"
                     /translation="MSEEKRRSGWFWFLLGGGLFVAFIVVVGLLGYAAMKAVGGNASF
                     GDKNIGVIDINGIIVSADNTVDQLRKFDEDDSIKAIILHINSPGGGAAASQEIYNEVL
                     HIRNEKKKPIVASIESMGASGAYYIASGANKIYANDASIVGSVGVIMEWTNYGDLLKW
                     AKLKPEVLKAGSLKDAGSPVREMTPEERAYFQSLLDNMHEQFIHDVAQGRGIPEATLK
                     PLATGQVWTGQQALPLHLIDKQGGFRDALLDTARQVGISGEPNVVKPPKKGRSLLDLI
                     TTDADDLWSGPGKLLEKNAGFYFLWK"
     gene            complement(14532..15065)
                     /locus_tag="VFA02_06870"
     CDS             complement(14532..15065)
                     /locus_tag="VFA02_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZU09607.1"
                     /translation="MKNRKEAGTPCSFHVLFAQRIGLALLLALTLSGCDRGSRPSQVA
                     TPAPNFSITDGAKTIRLSDYRGKIVVLNFWASWCAPCIEELPSLNALQRQMPQLVVLG
                     VDFNDDAPAYQQFLSDHHVTFQTIHDESQKSNLSFGTTRPPETYIIDGNGIIRRKFIG
                     PQDWTSPEIINYLKNLS"
     gene            15141..16169
                     /locus_tag="VFA02_06875"
     CDS             15141..16169
                     /locus_tag="VFA02_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU09608.1"
                     /translation="MELTVIIPARNEEDCLGTCLQSLVSQSDENWKLGRDWELLVVDD
                     HSADRTREIAAGFSDVTVLSAPACPRKWTGKANAVWTAARQAKGDWLLFTDADTIHEP
                     GDLRRALHEAEKHEVAMLSYSPRQIVKGFWQRALMPLVFSELALAYPPAKVSDPESRV
                     AAANGQFLLVRRDAYFQLGGHEAVAESILEDVELAYMAKRRKLGLRFRYAPDALSTRM
                     YRSFGQMCEGWTKNLALLFGNAVATAAWKVLDVLLLFGLPVLAWYFRWPISPTMPWLT
                     ARLLILLIWLRTLWRFYRRVMRSNFPLGDCLLSVFGLPLFIWLLWRSWFDHNVTKRVI
                     WKGREYGR"
     gene            16250..16660
                     /locus_tag="VFA02_06880"
     CDS             16250..16660
                     /locus_tag="VFA02_06880"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZU09609.1"
                     /translation="MILLTRKAEFSASHYYWNDAWPEEENRRVFGKCANKNGHGHNYT
                     LEVTVVGDVDTTSGFVVDLKQLKDIIEREVVSVYDHRHLNKEVPEFATAIPTTENIAI
                     AVWRRLEGKVPGAKLFRVRVYEMPDLFADYYGEP"
     gene            16657..17073
                     /locus_tag="VFA02_06885"
     CDS             16657..17073
                     /locus_tag="VFA02_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZU09610.1"
                     /translation="MRAYFSRRYRISASHRLHTDAFSAEENRLVYGKCNNPHGHGHNY
                     VIEVTLGGQVAPETGMVCNLVDLDACVRREVIERFDHTNLNFDPLFQERVPTTENLCI
                     EVFGLLQAAFDKAEVVRVRIEETSNNSFEYPAGAHR"
     gene            17092..17706
                     /gene="folE"
                     /locus_tag="VFA02_06890"
     CDS             17092..17706
                     /gene="folE"
                     /locus_tag="VFA02_06890"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZU09611.1"
                     /translation="MAKPAVIRKVMETGLENIATQDIYREIIRRFDEDPTRDGLVRTP
                     ERVEKALTFLTKGYRENPTEILRGALFDVDYDEMVIVKDIEMFSLCEHHMLPFFGKVH
                     VAYIPNGKVIGLSKIPRLVEVFARRLQVQERMTRQIADAIQEAIEPQGVGVVIEARHL
                     CMMMRGVEKQHSSTVTSAMVGVFQKQNTRNEFLALVRDRAPNGL"
     gene            17740..18615
                     /gene="truB"
                     /locus_tag="VFA02_06895"
     CDS             17740..18615
                     /gene="truB"
                     /locus_tag="VFA02_06895"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZU09612.1"
                     /translation="MNGLLIVDKPAGMTSHDVVARIRHAAGERSIGHLGTLDPMATGV
                     LPLLLGKFTRLAQFFGPLEKTYTGTVRFGFATDTYDAEGQPVGTPVSASLTLEQVRVA
                     ASRFWGEIEQMPPPFSAKKIGSTPAYKLARAGKEPKLKPVRITIKQFEVTSLDGDTAP
                     FLMTVSAGGYVRSVAHELGVALGCGAHLTSLRRIAAGPFLIEDAIPLAEIEAMAANGT
                     LRSRMPHPRTLLPELPSVTADAVSAGRLRHGAAVNLPEFSRASLVKIFEGQADLIAIG
                     RRVAGTLFQPIVVIA"
     gene            19063..22032
                     /locus_tag="VFA02_06900"
     CDS             19063..22032
                     /locus_tag="VFA02_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09613.1"
                     /translation="MVTIPGPLRSFLRMAGISQKIQAQDVLPLLARNIYLRGYEDGVE
                     TEFLRLLDRYVHQARELEAMAGPDGTIHTADCQQATQLLQVLGYQLRGSCGQKSAYLM
                     TASPERAFLTIDSGFPLTRLEQALQKNTPFFYAYPLSRVPVLFHESDWAGISPWRKKG
                     MTDLIDVLIHDPDVARLYSALAKNDEETREALRRSPGLKRLLPYAAVLDFYGSQIAVR
                     SGRVLVPGGANAEAGWKDLVGASPQSPGDFVNHLLARDKGWLAVYFDVLSRVSPEQQA
                     HLTESPRLKHLYEAYRSFGPEPSATKGVFRKGSELLILYSRLTWEQNGEPYVPGNLEI
                     WKEVLNQKADSKFVRDWARRSRGWNNPEQLLEALVAFSRIETDSGPVQVYLTLSEIDH
                     KRGHDKRLSDGAVRLIANKYTQFKSWYPIFSEFPDLNDTSIFNFVNVAEEIDKISNPS
                     LRANTLGAFQANIGLWQIMARQGEIPAAKMSASWQSVVEPFAKISTSPQLFDAARSSL
                     NGLILAAGGSTSYSQDDVIELLAGPPQQTPDAQRVRDELAARIHSVLDDQRLASLDTL
                     FALGDGLHQMAQGKGDNERMLALANQLKDFELPRPIFTKSEKIEWSPVVYSSRHAELQ
                     AKTDLAKLIKASPSPAQLEAARGQLAPFLRDTLVGLNYAYYEPPGAQILHNNPLFVRS
                     HDFAGITIIGAEQLWGPPDLIGVGTPAGGGAYLMGSLAELPYALATVEEDFISPENVQ
                     ALIWKEMVPNLLVDATMSRWWRVSQRELHAVALYQKSGQEILHAAASSPGLRPKVIEV
                     LSERMIPQRLEQVEEALQRPEALEAMRPPMLPADNFYLGAGFLKKFPADMASIGPASR
                     ELQELCSKDPTDTGLEKISQDFGVPHPTLTGNYSRELLDEKPFPVFGGYSYRLFGESW
                     ESSNLYWALLADEMGYSPAMLNELVPELTAHMVAKIFATDLDDWPAVARAMQEAGVEF
                     RLKKNTAVQVADVNASH"
     gene            22054..23115
                     /locus_tag="VFA02_06905"
     CDS             22054..23115
                     /locus_tag="VFA02_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU09614.1"
                     /translation="MTMKRLLSLGLLVGLFPGSRSGENFAVAQEDPSHILVNVVLVQL
                     NVAVTDRKGHYVTGLRPENFQVVEDKIPQKIATFEEGNGPVVKVNNVPLPASNLMAQN
                     NGPGKVPGTEGMKFTDSTANASNAPLGSLAGANVFILFDTSNYMYRGFVFAQDAIANF
                     IRSLEGVDKVAFYSYSRDLSRATMLTSDRAAIMQGVRSTVAGDDAALYNSLLMTVKDT
                     AQLHGRKAIVVFSNGPDNASSVPPEDVAELAQSTGTIIYMISTAAAQNDPVSTAVFER
                     MSKATGGKAYFAKNWEDEKRAFSSIREDLAHLYSISYYPQQNPNRGWRTISVKLVGKG
                     MEKYHVRTRDGYRLRQQTQ"
     gene            23279..24058
                     /locus_tag="VFA02_06910"
     CDS             23279..24058
                     /locus_tag="VFA02_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZU09615.1"
                     /translation="MTHSLIFAFFLFQAGPDTGAPPAPPSGSAIVEMLQNSGPVAIAV
                     LLLLLIASLYSWAIILGKWGSFKRARTQSNRFLRAFRKANRLQEIAAVSEQFKPSPLV
                     NVFDEVYETYRRQTGGYGPPKNITALERAAQTASSEALTVLEQRLTWLATIGAISPFI
                     GLFGTIMGIVDAFHGLGTAGAATLRAVAPGVSEALITTAAGLLVAVPAVIAYNQFTAR
                     CREFGARMDDFSRELLNSMEESELAPNQDPFEREAARGLHK"
     gene            24042..24464
                     /locus_tag="VFA02_06915"
     CDS             24042..24464
                     /locus_tag="VFA02_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU09616.1"
                     /translation="MAFTSRSGHTQTSLAEINITPLVDVVLVLLVIFMLTAPVLQSGI
                     DVAVPKTKTVKEITEQRLVLTIDKDQRVFLGDQPINLADLPSRLHQPNTDPAHQVIYL
                     RADERVPFGAFASVMDAVKQAGITNISIVTQPITNGPK"
     gene            24605..25432
                     /locus_tag="VFA02_06920"
     CDS             24605..25432
                     /locus_tag="VFA02_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZU09617.1"
                     /translation="MEPDRLGGPAVGSVALHLGIAGLIALYILVVGRFHGGLWGNNQS
                     APGAIQATLVSSAPTLPLPSEQKPTENVLATQQQSPAPAPPAPQKAEPVPPPEAIPIP
                     EKQKQPKVKQQAPQKQENPVKQAQKTTAPSQSKHPQLVQDQKNRAFYGEAQQSNIPRS
                     TTSNPNGNNPVAVNGGDFGSRFPWYVDIIKRKTAQNWLLQEVAPGTPAGARVYLSFLI
                     SRDGSPSHIRVEQSSGSPSLDSSCLRAVQRVDTYGPLPAGYTGSTLSVSYYCEEPGR"
     gene            25534..26832
                     /gene="tolB"
                     /locus_tag="VFA02_06925"
     CDS             25534..26832
                     /gene="tolB"
                     /locus_tag="VFA02_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZU09618.1"
                     /translation="MLTFSGLSAYSQDWVRTGTNLGAAKIRLAAANFKPTSTDPQTGE
                     LKNAFDTTLFNDLSNAGIFDMVSKSMAPPLMPGSPQEMNLAQWSAPPSNADMVAFGAI
                     GADNGKVTVLGYLFDAKNQQSPQILGKQYSDNASVNNVRNIAHRFADEIITRLGGIAG
                     ICETKIYYVSTHSGNKEIWAMDYDGENAHQLTHLGSISLSPRVSPDNSRVAFSALGKN
                     GWSIRMYSLLLNRIVSFPSPGGTTVSPAWSSDGSKLALSSSITGDPEIYTMSSDGTGL
                     HRVTAFRGPDVSPVWNPKTNAQIAWVSGRTGLPQIYIMDADGANVQRMTDGGYATSPA
                     WSPNGQFLAFAWNRKYGPGAPGGQDIYIMDIASKRWTQLTHDSGRCDFPSWSPDSRHI
                     VFQREDSSGHSEIWTMLADGTEQHQLTHGGSNSMPNWSWK"
     gene            26917..27657
                     /gene="pal"
                     /locus_tag="VFA02_06930"
     CDS             26917..27657
                     /gene="pal"
                     /locus_tag="VFA02_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZU09619.1"
                     /translation="MQVNHRKYIASAISLAALLAIAGCHKKKPAPPPPPPPTVSAPAP
                     TAEINVNPASINPGDSAVLTWKTTNATDVSIEGIGQVATSGTMNVKPTESTNYHLIAR
                     GDGGSADATARLTVNAAQPQSNLNEENMDDTLFHQNVHDIFYDYDSYEIRPDAQPTIQ
                     QDAAFLNQHPNIKVVIGGYCDERGSVEYNLTLGENRANAAKQALVNAGVSPDRLRTVS
                     YGKEKQFCTDHTEQCWQQNRRAQFNIDR"
     gene            27737..28630
                     /locus_tag="VFA02_06935"
     CDS             27737..28630
                     /locus_tag="VFA02_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09620.1"
                     /translation="MRKTIIHLTAFAALGLLCPVPRAHAASKEIIQLQTQVQTLQDML
                     QRMQQTNDSRMAVMQHLIEQTADNVNRMSQTVNGIQQQLQSQSENGVNGQQISGQIQS
                     LNDSVDELKSRISRLDKTLQDIQSQLQNVNAQPAGGAPGQQAAPGPGPDGQSAGQQPQ
                     APPVDQLYQGGIRDYNSAKYDVAAGEFGDVLKYYPQDNLAGNAQFYLGEIAYRQGDYK
                     TAIKNYDAVLEQFPGNAKAPAAQLRKAEAELALNQRDAGIRDLRSLIQRYPQTPEAAQ
                     ARSRLNGMGVRITAPKPSAYR"
BASE COUNT         6332 a         7423 c         8735 g         6520 t
ORIGIN      
        1 aagagagaac cttcagcaga tgattgtggt cacagggcgg ctggaaggtt cagacctggg
       61 gacggcgatg gcgcaggtgc agcgcgcggt cgaaaatctt catttgcctt cgaatgttcg
      121 tgtaacctac ggcggaacat acgaagaaca gcaaaaatcg tttcatgacc tgatgcgcgt
      181 gttgttgctg gcacttgcgc tggtgtttgg agtgctgttg acagagtttc gcaatttcgc
      241 tgctccggtc gcaatcctga cttcgtccat actttcaatt tccggtgttg tgattgcttt
      301 gctgataaca ggcactacat ttaacgtcgc atcctttatg ggactgatca tggtcatcgg
      361 tattgtggca aaaaatggga ttctgctgct cgatgcagat gaaaaattcc gtgctgaagg
      421 cgcgtcgccg cgcgatgcca tggtccatgc ggcacagcgc cggttgcggc ctattctgat
      481 gactgctatt gcggcagtct gcggaatgct tccgctcgca tttgcaattg gctccggctc
      541 acagatgctt cagccgctgg caattgccgt cattggaggg ctgtgtatct cgatggtgct
      601 gagcctgatc gtaaccccgg tagtgtatta tttcctgact cggcgcagag agcagcagat
      661 cttttaaaac gaatgcggat gtttcttaaa ctttgcaatc cggtacgcgg ccatcaactt
      721 tcagaagttt cgtgaaggcc gggcaattgc acggtcactg tcgttccctt tccgggctgg
      781 cttgtgatgc ggatgtcacc tccggctttg tccacaatgg ctttgcaaat cgagaggccg
      841 agtccggtgc ctccagtatt acgattgcgt gaggcgtctc cacggtagaa cggctcaaag
      901 acatgcggca gcgcttctgg cgggatgccg tctccctgat cggtgatttg aatttcagcg
      961 tatccattgc aggcatgacc ctgaatcgaa accgttgtcc cggaaacact atgctgcaac
     1021 gcatttagca ggaggttgga aaaaagcagg ctgcattcct gcggggccag gtgcgcggag
     1081 agcgcttcgg gcacaatgcg cgtaatggtg atgccgcggg tatcggcaat gggttgaagc
     1141 tgctcctgca cctgctgaag cgcctggccc acatctgtga cagaaggggc atgcgtctcc
     1201 gcagaggcgt cgacgcgggc cagggtgagc attttggcaa cgatttcttc catgcgctca
     1261 caatctgctt cgcagcgcgc aagcccggcc tgatattctt cggcgctgcg cggtttcatt
     1321 ccgagcaact gcaatgaaga cttgacgacg gccactccgg tcttaagttc gtgggcggaa
     1381 tcgctgacaa accggcgctg ctgcataaat gagcgttcca gccgctgtaa ggcagcttca
     1441 agggcctgga ccagagggaa aagctctttt gtggcgcatg cccgctcaga tggcgtgaac
     1501 ttccacgaat tgacagagac agtggatgcc tctgccgcaa gttcgtgcaa cggggcaagg
     1561 cttcggttga gcagccatgc catgactgct ccggtaacag ccagcaaggc caggctggag
     1621 atggcataaa actgtaccgt atcaatcaca gcgtgccata cgtgttttgt gggcgatccg
     1681 tagacaacag tgacatgatg aggcactccg ccgctggcat cattaggatc tacaattcgc
     1741 aagccgtggc gcagcaaaac gcgatagctc ctgccatgca accggagagg aaacatgcgg
     1801 tcctgctggg acgtgagcat ctgggcggat gcgccctccc agtttggcga gcgcccaagc
     1861 agatggcctt tttcgtcacg cacctcatag atgtcttcgc gcggtacggc gaggtctttc
     1921 atgtcaagca tgacattgtc ctcggcgtcg tcggcgtctt cgacagagcc gaggagagag
     1981 tcggcgcggc cgcgcagcat gatatcgaaa gcgcggaaat gactgtgacg ctcatagaac
     2041 caggccattc cggtgacaca gagggcagca atcagctcga ccagcaggac ggtgacgatg
     2101 agtcttcgcg ttatggaata gggcttcatg caatggcaag acggtagccc cgtccgcgca
     2161 gggtttcaat gctgggctgg ctggaaccgg catcgagttt tttacgaagg ttggagacgt
     2221 gcgcttcgat gacgttggag tggtgttccc agttgtagtc gtacagatgt tccagtaact
     2281 cgcgttttga gaccacgacg cgcgggcggt gaatcagata ttcgagaatg cggtactcca
     2341 tgggcgagag gtctacaatt tcgccagcac gccgtacaga ctgctcgagc gtatttactt
     2401 cgatatcggc cacagtgagt acgggatggg ccgcgccctt gccgcggcgg ataagggcct
     2461 tagcgcgagc aatcagttcg cccaggtcga aaggtttgct caggtaatcg tctgcgcctg
     2521 cattcagaag atcaataatg gagcgtgtct cgtcgcgggc ggtgaggacc agcacgggtg
     2581 tggtgctgtt cgatgagcgc agctttttca gcagagaaaa gccgtcgagc tttggcagca
     2641 tcaggtcaag aacgatgagg tcgtagcacg tatcctttgc aagaaaaaag ccgacctcgc
     2701 cgtcttcggc aatatctact gcgaagccag ggccatcgcg cagggcggcg gccacatttt
     2761 ccgcaattcg cggttcgtcc tcgacaacca atacgcgcat ggaaaccagt gtgcctcttc
     2821 ttagattaag ggtggctgaa cgtggccgtc gagcaggttt ttcagcatct taaatggatg
     2881 tttatgtttt atgaccgtag agatgcggga cggcgccttg ctaaacggct tcaggaatat
     2941 gcacgccgtg aagatgtagt tgtagcaggg cttcttcgag gcggcattcc ggtagcattt
     3001 gagattgcac aaaagcagca tgttccactg gatgtgttgc tggtgcgcaa actgggcgca
     3061 ccaaatcagc cggagctggc gatgggcgca gtggccccgg gtgggacgag gatacttgac
     3121 cactcgctga ttagaaaact ggggctcagc gaacagcagg tggacgagat gatttcagcc
     3181 gaggaagaaa aactgcaccg gggggagcaa ctctatgccg atgtgcgccc gcagattacg
     3241 gtcagggaca agactctgat tgcagtggat gatgggatcg ctacaggagc aagtatgatg
     3301 gccggggtgt ctgttctgcg cgcgcaggga gcaaaaaaag tggtcgttgc tgttcctgtt
     3361 gcgccgccac atgcggtgcg ggagttgtcg aaggtcgccg atgaggttgt atcgctgaaa
     3421 attacggact actttcctgc cgtgggattt ttctatcgcg atttccagca gactacggat
     3481 gaggaagtcc gcgacctgct caggcgggcg tctcagcata tctgatggaa tggaattccc
     3541 agtagtctgg ccagtttttc cagagtgtgc gcgatgtggc cgactcctac ggcgcagtgg
     3601 tgtgcggggc cctgcgcatt ccatttggcg gtgaactgct ttgctcccac agggaagcga
     3661 tagcggctgt tcgtattgcc gatttcgagg atgcttccgg aaacggattc gccttctgct
     3721 gcgattaatt tgaagctgtg cccggggtct tcgacgatgg atagcaatgt gacaggcccg
     3781 tgttttactg acatttctac ggaaagacca cggcccactt ttccgtggta gacctgtagc
     3841 ggcttcactt tggttttgcc ttctgcaatc gcgatgtgcc cagggccgtc gtggcccatc
     3901 agtacaacat cggcggcata gtctaatgcg tagtattcgg taaaggatcc acccacgttg
     3961 agtgcatcca tgatcttcat ggcgatagcg tttttgacct catattcacc tgcgacggga
     4021 atgtggcgtg aggtcagcag agatgttccg agaatgatgg aactcatcgt atcttcatta
     4081 gccggcacgc ccgtgccttt gtagtagcag gcaagggaag taagtctgtg ctttgctacg
     4141 aatgtatcga gtgcgacaga tgtccgtgct gcgcggcgca gttcttcgtc agagcaatca
     4201 ggctgaatgt caaaatgctg ctggaactct gagatgcgcc ttgtgatttc agcatcgggt
     4261 acttgttcac gtagcgcgct taactcgtct acttccagga cctcaacatc gccgccgaag
     4321 gtgatgcata tctgcgtgac atcggtcatg atgtcgagca tgccggaata gtagtgtccc
     4381 agcagaccca ggcggttgtt ctcaagctga gacttcactt tggctgcggc aagccagtct
     4441 tcaatttccg accatgtttg ctcatcgccc agaacgccaa tcgcttcgtg aaactgaatt
     4501 ccggcgcgcg cgaagacatt ggcgatttcc ggcacagggc aagcggagca ccatgccagc
     4561 cactctccag tcatggccgt gcggtcgttc aacttattga aggtggcgta atggatggca
     4621 ggtcccggct gaagattgag gacaatgact ggaacgccga tgcgttttac cagcggaagg
     4681 acggtgctcg aaagcgcgta agttgtggtg taaaggaaaa ggacctcaat ctcggcgcgc
     4741 cggaacgcgt ggccagcttc tctggcacga ccggtgctgt caatcatgcc gaggtttgtt
     4801 atatgaacat catgctgcgc gagacgtttt gcgactgtct gaatattggc ttccagacgc
     4861 tgcttcaagc ctgcaaattg gtcccagtag gcttcgaggc cgattccgca caggccaatg
     4921 ttgatatgag ttgtggatga catacagctc tcttatttgt ttcgatccaa gatgcagggc
     4981 cggcaagagt gcaattttct ttatggcaaa taaaatacct cgcgcatcat agggaagcgc
     5041 tcgccggaac gtaacccttc caggggagca aagtactctg ccatggcctg ctgccagcgt
     5101 gtgtttaccg ggctttgctc gatcctttgc catgtctgtt caaagtcatc tacgcgcatg
     5161 gtaaggacga gaatctcgtc gcggcgaaaa atggagtatt cgtggactcc agcgtttttg
     5221 aggagggcga gcatctccgg ccagacggcc tcgtgcgctt tgtcgtaagc cgggccggcg
     5281 ccggggcgta atttcaacat aaaggcaatt ctctgcatag gaatgaattc tactcagatt
     5341 tttggagata ttcggcgatt acacccaaca ctaggcacca caagagcacg tcaatcgtat
     5401 aggccacgcc tatgcgaagg atgacgctta tgatctctgc agagggtttt cctgttgggg
     5461 tccagccgac gagaagagat gagatccagg ttccgatgaa agcagctgcc agcgcatata
     5521 cccagtacag tactttgttg aaaacacggc ctatgttctt ccatctgccg tggcgcaggc
     5581 cagaggcaga ccctataagc gcaacaggaa gaagcagcgc agcaaatgtc cattgcagaa
     5641 aatcgtaaaa gtggtcttgc cagctcagga gccgaggata ggtaaagaac gcacgcatgc
     5701 cggcggaaaa ttttgaattc cagtatccgg cgtaaagaga ttcatgactg cgaagagcat
     5761 ccagtcccag caacagcagg tatccaacca cgatgacgac aacgagggca agccatcgct
     5821 gccagatggg cgcaggggcc ttggcccggt atagaagcct gaaggtgtgc gaatagagcc
     5881 aaaggaatgc gacgaccaga atcgcggcgg acagcatgga aaaaacgaat tgccatacgt
     5941 gcgaatcagg aatctgaagc catgcgaagc agagcaaaat gagcagcagg ttcccaatcc
     6001 actggaagag aaatactttg gcgtggaact ttggacgcgt cattgctttg cctccacgtc
     6061 aaggtgcagc atgtcactgg tggcctcgat tccgggctga tacatcagct ccacgcgcgc
     6121 gggactgatg ctgaagctgc ccggattcac gactttcagc agatagaagg actcgtggcg
     6181 tccatcaaat tcagtggcaa acatggctgc gcggtcgtcg tggaactcct ggcgggtgta
     6241 ccagaactgc catgtgtcgg gctggcccac gatattgtaa ctgtcactgt tctgaataaa
     6301 ttcagtgcca gcggggatag ggtcttcgat catcaggtat ttcacgggcg atccattggc
     6361 ggcaatgtgg acggcgagaa cgtctccggg agagaccggg cctttgagcg gttgtagctg
     6421 atagacgatc tggtcttttt tctgcgtggg aacaagtttg aagtaatcac gcgtgagatt
     6481 cagcgagaga ttgcctttct ggtagagctt cgtttccgtt gagtaataga tgccctgcgc
     6541 tgaccagtag gcacgtcccg tgccgtgctt cacaatctgg atgctgttgc cctgcggttg
     6601 caggtttgtc gcgggaatat ctacctgaag gctggccccg ctggttgcat cattcgcgtt
     6661 aaagtggcgt tttgcgactg aatggccgtt gacgagcaca tccacgtcaa agtcagcatt
     6721 gagctcctgc gaggtggcga ggtagtccgc aagtccgaag atgaccatgg cggtctgctc
     6781 ggtggaattc caccagtacc cgccgttacg gttcaggaca agccattgcg cagcctgttg
     6841 cagcaaggga tttttggggt cagtgtgggc gaggaagcgc atggcaaagg ccgtgctctc
     6901 cgcattgttg tcgtagtaaa gatcaagcag cgggttgtaa tcggatggcc agtatgcgag
     6961 cgctccagag cgctttgcct tatcttcaag cagctttgcg atttgcgagg cgcggctgtc
     7021 agaagtgtgg agcatggcga gtccggtgag cgcgagaccc tcggcggaga ggtcattgcg
     7081 gcgcgaccag agcgtgttca gagaatcgcc ggtattgcca gcgtctgctt ctgtgagcgc
     7141 atatacgaca taggcgcgca gttcagggag catccgcgga tgctcggcaa cggctttttg
     7201 caagtattca acgcctttgc cgaacatctg ctgcttcaca tcgtcgagcg ggtaggcttg
     7261 ttttgcacgc gccactccgc tgatgacata tgccgtcatg aagacgcggc tgtcgtcttc
     7321 tttccaccat ccccagccac catcgtcgtg ctggaaatcg gcaaggcggt caagtccggc
     7381 gcggactttg agctcaagat cattcaaatc tacgcgagag ggaagattga gtttcttcag
     7441 cgtctcagca atgatcacat caggcaggaa gctggacatg gtctgctcgg tgcatccata
     7501 gggatagctc gtcagataat cgagggcgga gaagatgctg ccagcgatgg aggggctgac
     7561 ttcgatgtgc aatttgtgtg cagctgggtc agtgtttgcc ggaaagttga tggaagcagc
     7621 cacctgtcca ttggcgtctt caacaacgcc gagttgattg agtttctgca ctactccgga
     7681 gggttccacc ggaaagctga tttcgagcgc gtcggactcc tgcgtgctga gtgctttggc
     7741 cagcaacgtc gctgtgccaa tctgcgaagc gcgcacgcgc cacaatacgg tcccgtcggc
     7801 cttgctgggg acggtgatgg tctgcggcgt gccggcgatg gtatccagtc cgtggatatc
     7861 aagcgaaagc tggatttgct ttgcctggtc cagataattg tggacgatga cggggatggt
     7921 caactcatcg cctttgcgca taaagcgcgg agcacccatg cgcacaatga tgtttttgcg
     7981 cacgagcacg cggttgatgg ctgctccggc ctttgaatca ggcgtgacgg cgcggaccgt
     8041 cgttcgccat gtcgtcagcg agtcagggaa ggtgaagctg acgcgcgcat ggccttgcgt
     8101 atccgtgtgg acattggccg cccaataggc ggtatctgga aatgctttgc gtactttggg
     8161 ttggacgaca tcgttgccgg gttttacctg cgcaagctgc gggcggtagc gcgactggcg
     8221 ttccgcgagc atgggggatt tagtgcctgc gcttccgctg aagtagtact gcaaggaaga
     8281 atcaaagggc gcgtagacgt aacgatccgg ataaagccgc ttgacgatgt cgccgctggt
     8341 atctggatag agatagtaga gggcctcatc cacaacgcca aagctcaggt cagcgctcac
     8401 cggcttgccc tggtagtcgc gtgtgtaaac gtcgtaagtg gcggactgct gcggctggaa
     8461 gacctgactg gccggagcga tttctatttg tagctgctcc tgcacagggg gcactttcaa
     8521 ctgtttgctt gcctgataca tctggtcgtt ctggatgtag actgcgctga cgtcgaggtt
     8581 gggctgcgaa tcggatgtga tgggcaggtc gaagctcagg gtcttgcctt cagacgaaag
     8641 cacttttttg aattcgacgg agtaccccga cgcgaccacg agcgcgtgga agttgtctac
     8701 ctgcgagatg atgctcaggt gcgcggtatc accgggggcg taggtcttct tgtcggcgac
     8761 gatctggacg gtctgtgagc ttccatacca gggagattcg ccgttgccca tcacccaaag
     8821 ccaggttgag tcctgcaccg tgcgtttctc aggagtgttc gcggaggctt ccacttccgc
     8881 gctgcctgcg ttctgggccg gaagtgaagc cgttgcctgc ccttgcgcgt ccgttgtcac
     8941 atcggtggaa ctgccttgtg tggtttccgt cttgccgttt acccagcggc ggtagacaag
     9001 ctgtaaatgt atctgtgtct gcacaggctt gttgtcatag tcaattgcgg tcaagcggaa
     9061 attcgccgcc tctcctgcgc ggacagagta gctggtcggt tctacgtgaa tgcggaagct
     9121 gccgtaagtg gcaaggaagc gtccgcggcc agtgatttcg cggttggcgg cgtccgtcac
     9181 tccggcttca atggtgtagt cctgatctga acgctgcttg ccgctggagt caaactttgt
     9241 gggcacggag atggtcagct tgccattggc gtcgagcttc ccggtttgct ccgattcctg
     9301 ctcagaatcg tagaggtcgg gctgctgctc gtcttcgccc acagcgggag gacctccggc
     9361 tgtgtcatcg ggttcgccct cgtcccacca gtagtggggc gagtggtaga tgcggtaggt
     9421 cacttcagcg ttcgcaaccg gctcgccgaa gaaatagcgc gaatctatga cgacctgatt
     9481 ggtttcgccc tgcaggacgc gcggttttgc cggaaccact cgcacctggt actcaggctt
     9541 tttgtactcc tcaacgcgga agctgcctcc ggctgtgtca tttccgtctc ccaggcgaat
     9601 agagtagtag ccgagcatgg cgttgttggc aagcgtcagg tcaccggtga cggtcccgtc
     9661 tttggatatg ggtaaatcct tttcaaagat ggaatggtct tcctggtcgg aaacgactgc
     9721 gtggatcgag ctgatcttcg gcagttccag atgattttct atccgctcgc gcaggatggc
     9781 cttccagtgg acagtgtgcc ctggacggta gacagggcgg tcggtataga cgaatgaggc
     9841 ccagcggttg ctttgcgttc cgctgaaggc gtagccggcc ggggtcactg ccgcgacgtc
     9901 tttgtcggcg gtggcgatga cccagatgtt gtcgcgctcc tgctgggaga caggcccatg
     9961 aagttcggcg aggccatcgg ggccggtttc tgcgctggcc tgctcgtgtg ggccgaaacc
    10021 gagcttcacc tgagcgtggg ggatgggcgc gccgctatgg cggtctacaa cgtatgcaag
    10081 tacctgtccg gagccggtac gggtcacgag cgctacacgg ctgaccataa ggacggtgta
    10141 tgccttgtag tggccgtccg tggcctcgac cagatagaag ccggaaggca gcggatctat
    10201 aggcaggtcc tgcgagtcgg agatgtaggt tggcgggact tcctgcctcc agcgtgctat
    10261 cagttgctga ctgttcaaca gaggaatctg cgcgaactga gcgacgccaa cgatgcggct
    10321 gcggtgcgcc agcgaagact gctttgcgcg gaggaagtcg cgtgtttcat aggagaattg
    10381 cctgcggaag aactgccgca cgaggaacca gagatgatgc ttccagtcgt gaaagcgctc
    10441 aagccacgtt ctttcatcaa tctgttcttt gggtgcccag ggtgtgccgt tttcaaagct
    10501 gtgcaggtcg cgcagattcg agagaaactt ttccggatcg ttgatgtggt aaacgcggaa
    10561 ctcaagctcg tctacgttat gcgtgtacag gtggatggaa ggtttttcgt ccggcgaaaa
    10621 cgtccggctg gtgctgaggt taaacgagac ggcgcgttct gcgtgtattg cctgagaggc
    10681 agcgatagac gccagcagaa aaaatagaga caggacaaat ctggtactac tcagtttccg
    10741 tcgcgcagaa tgttccaccg gtagacccca agaaagtttg aattttccga cacaggccgc
    10801 catcgggcat cgggatgatg cagcaggtct tcgatggcga cccgccgcac ctcgcccttc
    10861 gcattcccga tgggccctgt gtggtacaca acccaatctg aatgactgcc agtaacgacc
    10921 atagaatgat acggcgaatt ctgctcaagt tgacgaaaaa atatcagatc gccgggctgg
    10981 gccatccgga catcgcgact gatgaaatgt gtattaaaaa gcatcaggct ttttgcgtcg
    11041 gcaaactgag agaagctgcc gttgcttagg tcttccggga ggaatggtcc ggattttacg
    11101 cgaaagagag aagctcccaa cggtgtatcg gggtaatggt actgctggat ggagggcagc
    11161 ccggccagtt cttccatatg ctcaccgaca agccatccgg ggtcgtgtgc gtgcaaggcg
    11221 ttccggtacg cataacgcag gagcgcagcg caatcgttaa tctctgccgg gagcgcatct
    11281 tttgccttag ccgcctcaaa ttctgcaaga gcggtgaacc atgcgcggaa ggcgtggcgg
    11341 tcttcttcgg tatgtaaccg cagaaagtcc ggagtcccat ctgagaaact gtctgtgtag
    11401 tccgggagaa attgggcgtg aactgtaagc ggttttttct tccagtcgag atgaatcttc
    11461 tgctgcccgg acaagaccgg agagcgcaat tgcccctcga ttgtgcctcg atttttctgg
    11521 agcagccgca aattctcggc gttgtagcgg aattcggctg tcgagagcgg tcctccattt
    11581 gcgagccgga tgaggaatgc cgaatggaat ctgccatcag cgggcatagc gacttcccgc
    11641 ggaaggataa ttggtgttcg tgcgcgctcc tgatggagca gaccgagaaa gacagtggca
    11701 acaaccgctg cagtgaggat gaccagggtt gcggcttttc cggagcggcc tttcaccttt
    11761 gcaagtgtat ccaaaacaga taattttctt tcaatcactt acagacagag agagaaaaat
    11821 gcactctggc cagagttgga ggcaactttt gcatggaaat tgcgttcata cccaataaca
    11881 aatagaggca acttcaaatg cagtacacct caccaaagtt gttgagttca ctcttgaatt
    11941 gtccgcatac gcggatcctc agttgacact accctcaatg gcttcccttc aaattgcctc
    12001 tgaagcaggg cccgctggaa ggcgggcctt ttgctttgcc tgataatgat ggtatgccac
    12061 ttacagatca ggcaccgcga cagaacacga caaatgctgg gattgtacga tctccacagt
    12121 gggccggatt tcactggtta tggcacggct tcagtactcg aatgggaggg aagacaactg
    12181 tttacagcgg cgaacataag tcaggagaat tgaacctcgg cttcacccct gctgattcca
    12241 gagagacagt gctagccaac cgggaaacat tcattcgcga ccttactgaa ggccgcgcta
    12301 ctttgcctgc tttcgtaacg ctgaagcaga tgcattcgtc catcatccac gtagtcggca
    12361 aagcagatgc aggagaagag gccgttctaa cgggcgatgg catgctgacg ggggaagcgg
    12421 gagtgctgct gggaattcag actgccgatt gcattccggt gctggttgca gataaaaagc
    12481 agcgcgcggt agcagctttc catgcaggat ggcgcggcac cttaaagcgt attgtggaga
    12541 acggcatcgg gcggatgcgg ctggagtttg gcagcaagcc ggaagacctc tcagcggcca
    12601 tcgggcctgg gatcgggcag tgttgttatg cggtgggtga ggaggtccgc gatgcatttc
    12661 attcgcaatt tgcttatgcg caggacctct ttcgcgaagt ttataatgcg gaccccatta
    12721 aagaaaaata tcccatgctg tttttgaccg cgcgcgcgcc cggacactcg aatctcgggc
    12781 cgaatctcca tcttgatctg gtggaagcca accgcaggca gttactggac gccggactgc
    12841 gcgaggaaca gatatttgtg ctgggcgact gtaccagttg ccacgttgac cgctactttt
    12901 cttatcgtgc cgaacatggc tttacaggaa ggatgttgtc ggtgattggg gtgagagaca
    12961 gatccctttc ttaaggcatt ttcgcagatt ctgtacactc catcatttgc actgtgcagt
    13021 gtcgcagacg cccttttgtt tgcggcgact gtagtttcag ggctatccca attttttgtt
    13081 ggagttatgg gtgatgggtg gaatgaaagg cccacggcgt aaactctcac atcgccaaga
    13141 tttttccttc ggtgggcaga atgacggaca ttgtccggca ttctgcaaaa cccacatccg
    13201 aagttgacag gatttcaggc gggttgttcc gggcttccgc cttcactttt tggattcacc
    13261 atatcgcgca attctttgct gggcttgaaa taaggcacac gtttggcagg gacttcaact
    13321 ttggccccgg tttttggatt tcgtccagtg cgcggtttgc gctggcggat gcggaaacta
    13381 ccgaatccac gtatttcgat cttgtctcct gctttgagcg cctgaattac ggaatcaaaa
    13441 atcgtgtcta caatgacctc accgtcgcgt cgcgtcaggt cgcctaactg ggtcacttcc
    13501 tccaccaaat ctgctttcgt catggccggt ttcgctccac agaaattagg gtacgcctgt
    13561 taactataat agatgcaaca aatttgcctt atttccaaag aaaatagaag cccgcgtttt
    13621 tttcaaggag ctttcccggg ccggaccaga gatcgtccgc atcggttgtg atcagatcca
    13681 gcagagaacg gccctttttg ggcggtttta ctacatttgg ctccccggaa atgcctacct
    13741 gtcttgccgt atcgagcaga gcgtcgcgga atcctccttg tttatcaatc aggtgcaggg
    13801 gaagagcctg ctgtccggtc cacacctgac cggtggccag cggcttgaga gtggcctccg
    13861 gaataccccg tccctgggcg acatcgtgga tgaactgctc gtgcatgtta tccaaaagac
    13921 tttgaaaata cgcacgttct tccggggtca tttccctgac aggggagccg gcatctttca
    13981 gcgatccggc tttgaggact tccggcttca gttttgccca tttgagaagg tcgccgtaat
    14041 tggtccattc catgatgacg ccgacggagc caacgatgct ggcgtcattg gcgtaaattt
    14101 tgttggcacc cgaagcaatg tagtaggccc cggaagcgcc catgctttca atggaagcga
    14161 cgatgggttt ctttttctca ttgcggatat ggaggacctc attgtaaatc tcctgcgagg
    14221 cagctgcgcc tccgccggga gagttgatgt gcaggatgat ggctttgatg gaatcgtcct
    14281 catcgaactt gcgcaactgg tctacggtat tgtcggcaga aacgatgatg ccgttgatgt
    14341 caataacccc gatgtttttg tcgccaaagc tggcgttgcc gccaacggct ttcattgcgg
    14401 cgtatccaag gagtccaacc acaacgataa aggcgacaaa gagaccgcct ccaagcagaa
    14461 accagaacca acccgaacgg cgcttttctt cgctcatagg ggcgagttta gcacttacag
    14521 catgtcaaca gctaagaaag gtttttcaga taattgatga tctcggggct cgtccagtcc
    14581 tgcgggccga tgaacttacg gcggatgatg ccgttgccgt caatgatgta ggtttcaggc
    14641 ggacgggtgg taccgaagga cagattgctt ttctgcgatt cgtcgtgaat ggtctgaaat
    14701 gttacgtgat ggtcagagag gaactgctga tatgcaggtg cgtcgtcatt gaagtcgacg
    14761 cccaggacga cgagctgtgg catctgccgc tgcagggcat ttaacgaggg cagctcttcg
    14821 atgcaggggg cgcaccatga cgcccagaag ttgaggacga cgattttgcc gcggtagtcg
    14881 ctgagtcgga tggtctttgc gccatcggtg atggaaaagt tgggggcggg agtagcgacc
    14941 tgcgatgggc gagagccgcg gtcgcatcct gagagggtga gggccaggag gagagcgagt
    15001 cctattcttt gcgcgaaaag gacgtggaaa gaacagggcg tgccagcttc tttcctattt
    15061 ttcaaggcct ggggcagcca tttccaaaga tgaaaatcta accagctctg cacgtttcta
    15121 taataaattc cggggcctgc ttggaactta cggttattat tcctgcgcga aatgaagagg
    15181 actgtctggg cacgtgtctc cagtcgctgg tttcgcaatc tgacgaaaac tggaaactgg
    15241 gccgtgactg ggagttgctg gtggtggacg accactctgc ggatcgcacg cgggagattg
    15301 cagcagggtt ctcggatgtg acggtgttaa gcgcacctgc ttgtcccagg aagtggacgg
    15361 gcaaagcaaa cgctgtctgg acggcggcca gacaggccaa aggggactgg ctgctgttca
    15421 cggatgcgga cacgatccat gaaccgggtg atctgcgccg cgcattgcac gaggcggaaa
    15481 agcacgaagt ggccatgctt tcgtattcgc cgcggcagat tgtgaagggc ttctggcagc
    15541 gggcactcat gcccctggtc ttcagcgaac tggctctggc atatcctccg gccaaggttt
    15601 ctgatccgga atcgagggtc gcggcggcca acgggcaatt tctgctggta cggcgggatg
    15661 cgtattttca gctcggcggg catgaggcag tggcggagtc cattttggaa gacgtagagc
    15721 ttgcctatat ggcaaagcgg cgtaagctcg ggctgcgctt ccggtacgct ccggatgcgc
    15781 tgagcacgcg catgtaccgc agttttgggc agatgtgcga aggttggacg aagaacctgg
    15841 ccctactgtt tggcaatgca gtggcgaccg cggcgtggaa ggtgctggat gtgttgctgc
    15901 tgtttgggct gccggtgctg gcgtggtatt ttcgctggcc gataagcccg acgatgccgt
    15961 ggctaacggc gcgcctgctt attctgctga tatggctgag gacgctgtgg cgcttttacc
    16021 gccgggtcat gaggtcgaat tttcccttgg gagattgcct gttaagcgta ttcgggctgc
    16081 cgctgttcat ctggctgctg tggaggagct ggttcgacca taacgtgaca aaacgcgtaa
    16141 tctggaaagg tagggaatat gggcgttgat cctttgttgc tcctcaagat gacagattga
    16201 cttttctgtt tggggatggt cagtaattca tttgtcatga cgataaacga tgattttact
    16261 tacgagaaaa gctgaatttt cagcttcgca ctattactgg aacgatgcgt ggccggagga
    16321 agagaaccgg cgcgtgtttg gcaaatgcgc caataagaat ggtcacgggc ataattacac
    16381 gcttgaagtg acggtcgtag gcgatgtgga tacgacgtcg ggttttgtgg tggatctgaa
    16441 gcaactcaag gacataattg agcgcgaggt ggtgagtgtg tacgaccatc ggcacctgaa
    16501 taaggaagtg ccggaatttg ccacggccat tccgacgacg gaaaatatcg cgattgcggt
    16561 atggagacgt ctggaaggga aggtccctgg agcgaagctg ttccgggtgc gcgtgtatga
    16621 aatgccggac ctttttgccg attactacgg ggagccatga gggcgtattt ttcacggcgg
    16681 taccggatca gcgcgtcgca caggctgcat acagacgcat tcagcgcgga agagaaccgc
    16741 ttggtctacg ggaagtgcaa caatccgcac ggacacggac ataactatgt gattgaggtg
    16801 acgttgggag ggcaggtcgc cccagagacc ggaatggtgt gcaatctggt ggacctggat
    16861 gcgtgcgtaa gaagggaagt catcgagcgt tttgaccata ccaacctgaa ttttgatccg
    16921 ctttttcagg agcgcgtgcc gactacggag aatctatgta tcgaggtctt cggcttgttg
    16981 caggcagcct tcgacaaggc ggaagtggtg cgtgtccgga ttgaggagac cagcaacaat
    17041 tcctttgaat atccagcggg cgcgcacaga tagacaagaa agagagcaga gatggcgaaa
    17101 ccggcagtta tccgtaaggt catggaaacg ggccttgaga acatagccac acaggacatt
    17161 tatcgcgaga tcattcgccg ctttgatgag gaccccacac gcgacgggtt ggtgcgcact
    17221 ccggagcgtg tggaaaaggc gctgacgttc ctgacgaagg gctaccgaga aaacccgaca
    17281 gaaattctgc gcggggcgct ctttgatgtg gactatgacg agatggtgat tgtaaaggac
    17341 atcgagatgt tctcattgtg cgagcaccac atgctgccat ttttcggaaa agtgcatgtt
    17401 gcctatattc cgaatggaaa agtgattggt ctgagcaaga ttccgcgcct ggtggaggtg
    17461 tttgcccgcc gtttgcaggt gcaggaacgg atgacgcgcc agattgccga tgccattcag
    17521 gaggccattg agccgcaggg cgtaggcgtg gtgattgaag cacggcacct ttgcatgatg
    17581 atgcgcgggg tggagaagca gcactcctcg acggtgacat cggcgatggt aggcgtcttc
    17641 cagaagcaga atacgcgcaa tgaatttctg gcgctggtgc gcgaccgggc gccgaatggc
    17701 ctgtaaattc cacacgctcc gggcagctat agtagtcagg tgaacgggct gttgatcgta
    17761 gacaaacctg cggggatgac gtcgcacgac gtggttgccc gcattcgcca cgcagccgga
    17821 gagcggtcca ttggtcatct ggggacgctc gatccgatgg cgacgggagt cctgccgctt
    17881 ttgctgggaa aattcacccg gctggcgcag ttttttggtc ctctcgagaa gacctatacc
    17941 ggaacggtgc ggttcggttt tgctacggac acatacgatg ccgaggggca gccggttggt
    18001 acgcctgttt cggcttcact cacgctggag caggtgcggg tggctgcgtc gcgtttttgg
    18061 ggcgagatag agcagatgcc gccgccattt tccgccaaga agattggcag tacgccagcg
    18121 tataagctgg ccagggctgg gaaagagcca aagctgaagc ctgtccgcat cacaatcaaa
    18181 caatttgaag tgacctcgct tgacggcgac acggcaccgt tccttatgac tgtttccgcg
    18241 ggcggatatg tgcgaagcgt agcgcatgag ctgggagtgg cgcttggatg cggggcgcat
    18301 ctgaccagcc tgcgccgcat tgccgccggg ccatttctga ttgaggatgc gattccgctt
    18361 gctgagatcg aggcaatggc ggcgaatggg acgttgcgtt cacgaatgcc tcatccgcgc
    18421 acgctattgc cggaattgcc ttcagtcact gcggacgctg tttcggcggg aagactgcgg
    18481 catggggcgg cggtaaacct gccggagttt tcgcgcgctt cgctggtgaa gatatttgaa
    18541 gggcaggcgg acctgattgc cattggacga cgtgttgccg ggacgctgtt tcagccgatt
    18601 gtggtgattg cgtagaccgg gccggattac gagatcatca catgccggga gaaaaaccct
    18661 ctacccgccg gagcagatta caactgtctg gaagacattg ttcgtcttac aaaagcaggt
    18721 tcgtttaaaa ctctcttcga ttgttggacg tgaatggtat gatgctctcc gaacaaacaa
    18781 gggactgcga gacagccgca taaataaaac ggtttcctga ctggcgaaac gtgaacgcgc
    18841 tttcagcctt gcggataaac cggcgtctgt tgtctgcaca ctcctcgact gctctcaaag
    18901 tcctggaagg ggcgttgggc catgaggtct tatctatgcg tccgggcgtt tctcgccgct
    18961 tttttgtccc tgtgcttctg cgcttctgct caggagcctt ccactgcgtc tgattctgag
    19021 acgggaccgg tcagccaaac gttagcttcg ccatccgggg atgtggtcac aattcccggt
    19081 cctctgcgtt catttctgcg catggccggg atcagccaga agatccaggc gcaggacgtg
    19141 ttgccgctgc tggcgcggaa tatctacctg cgcggttatg aagatggcgt agagacggag
    19201 tttctgcgat tgctggaccg ctatgtgcat caggccaggg aactggaggc gatggcaggc
    19261 ccggatggga ccatccatac ggctgactgc cagcaggcga cgcaattact tcaggtgctt
    19321 ggctatcagc ttcgcggaag ctgcggccag aagagcgctt accttatgac ggcaagcccg
    19381 gaaagagcat tccttacgat tgattccggg tttccgctga cacggctgga gcaggcgctc
    19441 cagaagaaca ccccattttt ctatgcctat cctctgagcc gcgtgcctgt acttttccat
    19501 gagagtgact gggccggcat cagtccatgg cgcaagaagg gcatgacgga cttgattgac
    19561 gtgctgatac atgatccgga cgtggcgcgt ttgtattcgg ccctggccaa gaatgatgaa
    19621 gagacgcgag aggcactgcg ccgttcgcca ggactgaaac ggctgcttcc ttatgccgcg
    19681 gttcttgatt tttacggcag ccagattgcg gtccgttccg ggcgtgtgct tgttcccggg
    19741 ggcgccaatg cagaggcagg ctggaaagac ctggttgggg caagtccgca gtctcctggc
    19801 gactttgtga accatctgct ggcgcgggac aaggggtggc tggcggtgta tttcgatgta
    19861 ctgtcgcgcg tcagtccgga gcagcaggca caccttacgg aatctccgcg gctgaagcat
    19921 ctgtatgagg cctaccgctc gtttgggccg gagcctagcg cgaccaaagg agtgttccgg
    19981 aaaggctccg aactgttgat tctgtattcc cgcctgacgt gggagcagaa tggcgaaccg
    20041 tatgttccgg gcaatcttga aatatggaaa gaggtcctga accagaaggc cgattccaaa
    20101 tttgtgcgcg actgggcgcg acggtcgcgg ggctggaaca atccagaaca attgcttgag
    20161 gcgctggtgg ccttttcgcg gattgaaact gacagcgggc cggtacaggt gtatctgacg
    20221 ctgagcgaga tagaccacaa gcgcggccat gacaagcgcc tttctgatgg cgctgtgcgt
    20281 ctgattgcca acaaatacac gcagtttaag agctggtatc cgatcttctc agaatttcct
    20341 gatctgaacg acacatccat tttcaatttt gtgaacgtag cagaggagat agacaagatt
    20401 tccaatccct cactgcgcgc aaatacgctg ggagctttcc aggcaaatat cgggctgtgg
    20461 cagatcatgg cgcgccaggg agagattccc gccgcgaaga tgagcgcgtc gtggcagagc
    20521 gttgtggagc cgttcgcaaa gatttcgact tcgccacagc tttttgatgc ggcccgcagt
    20581 tcgctgaatg gactcattct ggctgcaggc ggcagtacaa gctattcgca ggacgatgtg
    20641 attgagctgc tcgcaggacc gccacaacaa acgccagacg cccagcgcgt gcgggacgag
    20701 ttggctgcga gaatccattc tgtgctggac gaccagcggc ttgcctcgct ggatactttg
    20761 tttgcgctgg gagatggtct gcaccagatg gcgcagggga agggcgacaa cgagagaatg
    20821 cttgcgctgg ccaaccagtt gaaagatttt gagctgcccc ggcccatctt taccaagagt
    20881 gaaaagattg agtggtcgcc tgtggtctat agcagccgcc acgcggaatt acaggcgaag
    20941 acagacctgg cgaaactcat caaagcttcg ccttctccgg cgcaactgga agcggcccgc
    21001 ggtcagcttg cgcctttcct gcgcgacacg ctggtagggc tgaactacgc ctattacgag
    21061 ccgccgggcg cgcagattct gcacaataat ccgttgttcg tgcgttccca tgactttgcc
    21121 gggattacga tcattggggc cgaacagctc tgggggcctc ctgacctgat tggtgtaggg
    21181 accccggccg gaggcggtgc gtatctgatg ggatctctgg ccgaactgcc ttatgcgctg
    21241 gcaactgtgg aagaagactt tatctcaccg gaaaacgtgc aggcgctgat ctggaaggag
    21301 atggtcccga acctgctggt ggacgcgacg atgtcgcgct ggtggcgcgt gagtcagcga
    21361 gagcttcatg ccgtcgcgct ttatcagaaa tcaggccagg agatacttca tgctgcggca
    21421 tcgagcccag gcctgcgccc gaaggtcatc gaggtgcttt ccgaacggat gatcccgcag
    21481 cgtctggagc aggtagagga ggcattgcaa agacctgaag cgttggaagc tatgcggcca
    21541 ccgatgcttc ccgcagacaa tttttatctt ggggcagggt ttttgaaaaa gttcccggca
    21601 gatatggcgt ccattgggcc tgcgagtcgt gagttacagg agctttgcag caaagaccca
    21661 acagatacgg gtctggagaa aatatcacag gacttcggag tgcctcatcc tactttgacc
    21721 gggaattatt cacgcgagct tctggatgaa aagccatttc cggtattcgg aggatattca
    21781 taccggctgt ttggagagtc gtgggagtca agcaatttgt attgggcact gcttgcagat
    21841 gagatgggtt attctccggc gatgctgaac gaactggtgc ctgaattgac ggcgcatatg
    21901 gtggcaaaga tttttgcgac tgatctggat gactggccag cagtggcgcg cgccatgcag
    21961 gaggccggcg ttgaattcag actgaagaaa aatacggcag tgcaggtggc cgacgtaaat
    22021 gcttcacatt aggtgtgtgt tgacgggtgc tctatgacaa tgaaacggct cttatctctg
    22081 ggtttgcttg ttggtctttt tccaggcagc agatcgggcg aaaattttgc tgtcgcccag
    22141 gaagacccat cgcacattct ggtgaatgtg gtcctggtgc agttgaatgt ggccgtgact
    22201 gaccgtaaag gccattatgt tactggactg cgtccggaaa attttcaagt ggtcgaagac
    22261 aaaatcccgc agaagattgc cacgtttgag gagggcaatg gtccggtggt gaaggtgaac
    22321 aacgttccgc taccagccag caaccttatg gcgcaaaata atggcccagg caaggtgccg
    22381 gggaccgaag ggatgaagtt taccgacagc actgcgaatg catcgaatgc accgctcggg
    22441 tcgctggctg gagccaatgt ctttattctt ttcgatacca gcaactacat gtaccggggc
    22501 tttgtgtttg cgcaggacgc gattgcgaac ttcatacgct cgctggaagg cgtggacaag
    22561 gtagcgttct attcttatag ccgcgacctt tcgcgggcca ccatgcttac atcggaccgg
    22621 gcggccatta tgcagggcgt gcgcagcacg gtcgcgggag atgatgcggc gctgtataac
    22681 tcactgctga tgacggtgaa agatacggcc caactgcacg ggagaaaggc aatcgtggtt
    22741 ttctcaaacg ggcctgacaa cgccagctca gtgccgccgg aagatgttgc agagctggcg
    22801 cagtccacag ggacgatcat ctacatgatc agcacagcgg cggcgcagaa cgatccggtt
    22861 tcaacggcag tctttgagcg catgagcaag gccaccgggg gaaaagccta ctttgcaaag
    22921 aactgggaag atgagaaacg ggccttttcg tctatccgcg aagacctggc gcatctttat
    22981 tccatcagct attatccgca gcaaaatccc aaccgtgggt ggcgcaccat ttcagtcaag
    23041 ctggtaggga agggaatgga aaaatatcac gtgcgcacgc gcgatggata tcgcctgagg
    23101 cagcaaacgc agtaaacggc gatagcgcat cgtcagctaa gatgcggtca gcgcacctac
    23161 ggcgctgtta gctaaaaggc ggtcagcgcg ctctatgaaa ccaacgtaac catttcaatt
    23221 cctgttcgtc ggacaacttg gaaggggaga ttcgcgtctg ctaacgttga tacaggcaat
    23281 gacacattct ttgatattcg cctttttttt gtttcaggcg gggccggaca cgggcgctcc
    23341 gcctgcgcct cccagcggca gcgcgattgt ggaaatgctc cagaacagcg gcccggtggc
    23401 cattgctgtt ttgctgctgc tactgattgc gagcctttat tcatgggcaa tcattctggg
    23461 caaatggggc agctttaagc gcgcgcgcac gcagagcaac cgctttcttc gtgcctttcg
    23521 caaggccaat cgtttgcagg agattgcagc ggtcagcgag cagttcaaac ccagtccgct
    23581 ggtgaatgtg tttgatgaag tgtatgagac gtaccgtcgc cagacgggag gctacggacc
    23641 tccgaagaac ataacggcgc tggaacgcgc ggcgcagacg gcctccagcg aagcgctgac
    23701 tgtgctggaa cagcggttga cctggcttgc caccattggt gcgatttcgc catttatcgg
    23761 attgtttggg accattatgg gcatcgtgga cgccttccac gggctgggca cggctggagc
    23821 agccacgctg cgcgctgtgg ccccgggcgt ttccgaagcg ctgattacga ctgctgctgg
    23881 tctgcttgtt gcagttcccg cagtgattgc ttacaaccaa ttcacggcgc gctgccgcga
    23941 gtttggggcg cgcatggacg acttttcgcg cgaattgttg aacagcatgg aagagtcgga
    24001 actcgcgccg aaccaagacc cgtttgaaag ggaggccgcg cgtggccttc acaagtagaa
    24061 gcggacacac gcagacatcg ctggcagaaa tcaacattac gccgctggtg gacgtggtgc
    24121 tggtgctgct ggtgatcttt atgctgacgg ccccagtgct gcaatcggga attgatgtcg
    24181 ctgttccgaa aacaaagacg gtgaaggaaa ttaccgagca gcggctcgtg ctgacaattg
    24241 ataaagacca gcgcgtcttt cttggcgatc agccgatcaa tctcgccgat ctgccttcgc
    24301 gtttacacca gcccaataca gaccccgcgc atcaggtaat ttatcttcgc gcggatgagc
    24361 gcgtcccttt tggggcgttt gcttcggtga tggatgcggt gaagcaggcg gggattacga
    24421 acatcagcat tgtgacgcaa ccgattacga acgggccgaa gtgagagtgg tttatctcac
    24481 cctttgcttg gcgaaggata gggcaaatgg cctgaagcgc tgcgcgccct gccgatgctt
    24541 tgctcagagc ggcaactttt cagagatttt ttagagtgtg atgagaatca gcgcgagaat
    24601 tgacatggaa cctgaccgcc tcggcgggcc cgcggtgggt tcggtcgccc tgcatctggg
    24661 gattgcggga ctgattgcgc tttatattct ggtggttgga cgctttcacg gcggactgtg
    24721 gggcaacaac cagtccgctc ccggtgccat tcaggcgacg ctggtcagtt ctgcgccgac
    24781 actgccgctg ccgagtgaac agaagccaac ggagaacgtc ctggcgacac agcagcagag
    24841 tccggcacct gcgcctcctg ctccccaaaa ggccgagccg gtccctccgc cggaggcgat
    24901 tccgattcca gagaaacaga agcagccgaa ggtaaagcag caggccccgc agaagcagga
    24961 aaatccggtg aagcaggcgc agaagaccac agcgccatcg cagagcaagc atccgcagct
    25021 ggtacaggac cagaagaaca gggcattcta cggagaagcg caacaatcga acattccgcg
    25081 ctcgacgaca agcaacccaa acgggaataa tcccgtcgca gtcaatgggg gagactttgg
    25141 ttcgaggttt ccctggtacg tggacattat taaaagaaaa actgcgcaga actggttgtt
    25201 gcaggaagtg gctccgggta cgccggctgg agcgagagtg tatttgtcgt ttcttatttc
    25261 gcgcgacggt tctcccagcc acatacgagt ggaacaatcg agcggctcac cgagtctcga
    25321 ctcttcatgt ctaagagcag tgcagagggt tgacacttat ggccctctcc cggccggata
    25381 caccggcagc acgctgtcag tgtcgtacta ttgtgaagag ccgggacgat gatcctctcc
    25441 agcttttgta ttttcagtct gcgggacgga agaatgaatt caggcaagaa gcaatctttt
    25501 ttcagtttgt attgtttttc atttttagtt ttcatgctta ccttcagtgg gctctctgca
    25561 tattcgcagg actgggtacg cacgggcaca aatctgggtg cagcgaagat ccgccttgct
    25621 gctgcaaatt tcaagcctac atctactgat cctcagactg gcgagctgaa gaatgcgttt
    25681 gacacaacgc tgttcaatga tctgtcgaat gccgggatct tcgacatggt ttcaaagagc
    25741 atggcgcctc cactgatgcc aggttctccg caggagatga atctggcgca gtggtccgca
    25801 ccgccttcca acgctgatat ggttgcgttt ggtgcaattg gcgcggacaa cggcaaagtg
    25861 accgttttgg ggtatctttt cgatgcgaag aaccagcagt cgcctcagat tctcggcaag
    25921 caatatagcg acaatgccag cgtaaacaac gttcgcaata ttgcgcaccg ctttgcggat
    25981 gagattatta cgcggctggg tggaattgca ggcatctgcg aaacgaagat ttattacgta
    26041 tccacgcact cgggcaacaa ggaaatctgg gccatggact acgatggcga aaacgcgcac
    26101 cagcttacgc atctgggatc tatttcgctt tctccgcgtg tttcccctga caattcacga
    26161 gtggcatttt cagcgttggg gaagaacggc tggtccatcc ggatgtactc gctgctgctg
    26221 aaccgtatcg tgagtttccc gtcgccgggt ggaacgacag tttctcctgc gtggtcgagc
    26281 gatggatcga agctggcgct ttcctcttcg attacaggcg atccggagat ctacaccatg
    26341 agttctgacg gaacaggact gcaccgcgtg accgcattcc gcggaccaga cgtctctcct
    26401 gtatggaacc cgaagaccaa cgcgcagatt gcctgggtga gcggacgcac cgggctgccg
    26461 cagatttaca tcatggatgc cgatggcgcg aatgtgcagc gcatgaccga tggtggatat
    26521 gcgacatcgc ctgcatggtc tccaaacggg cagttcctgg cctttgcgtg gaaccgcaag
    26581 tatgggccgg gtgctccggg cgggcaggac atctatatta tggacatagc cagcaagcgt
    26641 tggacgcaac tgacgcatga ttccggacgc tgcgactttc cgtcgtggtc tccggattcg
    26701 cgccacatcg tttttcagcg tgaagacagc agcgggcact ccgagatatg gaccatgctc
    26761 gccgacggca ctgagcaaca ccaactcacg catggtggct cgaactccat gccgaactgg
    26821 agctggaaat aaaaattttt aagtaccgcg gcaggaacgt tccttgccgt gctgtcctgg
    26881 atgaaaatac agatacaaca ctcctggagg agaaacgtgc aagtcaatca tcgcaagtac
    26941 atcgcttccg caatcagtct ggcggcgctt ttggcgattg ccggatgtca taagaaaaag
    27001 ccagcaccgc ctcctccgcc accgccgacg gtgtctgctc ctgcccctac ggctgagatc
    27061 aatgtgaacc cggcctcgat caatcctggt gacagtgctg tgctcacctg gaagactacc
    27121 aatgccacgg atgtttcgat tgaaggcatt ggacaagtgg cgacctcagg cacgatgaat
    27181 gtaaagccga cggaatccac aaactatcac ctgattgccc gcggcgatgg cggttcggct
    27241 gacgcaacgg cgcgcctgac ggtgaacgcc gcgcagccgc agagcaatct gaatgaagaa
    27301 aatatggacg acacgctctt ccatcagaac gtccatgaca tcttctacga ttacgacagc
    27361 tacgaaatcc gtccggacgc acagccgacc atccagcagg atgcggcatt cctgaaccag
    27421 catccgaata tcaaggtggt gattggtggc tactgcgacg agcgcggatc agtggaatac
    27481 aacctgacgc tgggtgaaaa ccgcgccaat gccgccaagc aggcactggt caacgccgga
    27541 gtaagtcctg accgcctgcg cacggtgagc tacggcaaag agaagcagtt ctgcaccgac
    27601 cacacagagc agtgctggca gcagaaccgc cgcgcacaat tcaacattga ccggtagctc
    27661 catctgcaga acggcagcat tgttcaccac agtgctgccg tttgcttttg gtctacactt
    27721 taagtgggga ctttctatgc gaaagacaat catccacctg accgcttttg ccgctcttgg
    27781 cctcctctgt cctgttccgc gcgcccatgc cgcgtcgaag gaaatcatcc agttgcaaac
    27841 gcaggtgcag acccttcagg acatgctcca gcgcatgcag cagaccaatg attcacgcat
    27901 ggctgtgatg cagcacctca tcgagcagac cgctgacaat gtgaatcgca tgtcgcagac
    27961 ggtaaacggc atccagcaac agcttcagtc gcagagcgag aatggcgtta acggtcaaca
    28021 aatttccggc cagatacaga gtctcaatga ttcagtagac gaactgaagt cgagaatttc
    28081 acgtctggac aagaccctgc aggacattca gtctcagctt cagaacgtca acgcgcagcc
    28141 ggcgggtggc gctccggggc agcaggcagc ccccggcccc gggcctgatg ggcagagtgc
    28201 cggacagcag ccgcaggccc caccggtaga ccagctttat caggggggca tccgcgacta
    28261 taacagcgcg aaatatgacg ttgcagcggg cgaatttggc gatgtgctga aatattatcc
    28321 gcaggacaac cttgccggga acgcgcagtt ctatcttggc gaaattgctt atcgtcaggg
    28381 cgactacaag acggccatca agaattacga tgcggtcctc gagcagttcc cgggcaatgc
    28441 caaggcgccc gcagcgcaac tgcgcaaggc cgaggcagag ttggcgttga accagcgtga
    28501 tgcgggcatc cgcgatctgc gcagcctgat tcagcgctat ccgcagaccc cggaagcagc
    28561 gcaggcgcgc agcaggctga atggaatggg tgtgcgtatc acagcgccaa agccgtcggc
    28621 atacaggtag ccccctacaa ctcacctatt gcaggcagca tcagaagaaa agcctatgca
    28681 aagtcacgac agcgactcga agaaaggggc ggtcgaatcc gaatcaccgc gcgaccactc
    28741 ccgcgactat ttgaaagacc aacttcctca ccgttctgcc gacccgatga ttaaggaccg
    28801 agacgaagac cacgatactg actttccaga accaggcagg gtaccagaac acagcggtca
    28861 gcacgagtaa ggactacgcg cgaatctttt catcactgat ttcatccaaa agaggaaacc
    28921 gggagatgaa acgatggcgg ctttttgtgc atgttgcgga gcagaaatta cacctaaggc
    28981 agaggcttgt gcagtatgcg gcactccgca
//
LOCUS       DASRBV010000181         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_570018, whole genome shotgun sequence.
ACCESSION   DASRBV010000181 DASRBV010000000
VERSION     DASRBV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570018"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VFA02_06940"
     CDS             <1..1468
                     /locus_tag="VFA02_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HZU09621.1"
                     /translation="QFTTDDARLMQTYTAALSVLQRNITQLHPYSGPVLVEGSVYPGV
                     WLECAPQEGEVYSAIRPDIARNNHMVFFALQKEDGQLPCSVKMDSIGFGQIQMVVPIA
                     ATAWELARKSGDNELLDKAYLACSRWDAWLRRYRNTRGTGLCEGFCTYDTGHDNSPRW
                     KGIPNRCPDADARKCPPVPGMPRLCPDLSASVYGGRVALAAMAKALGRQSESDRWTED
                     AESIRKLMIEKLYCPEDAAFYDLDTEGHFIRIRGDVISRVLGEHVVDSRLFDIIYQKQ
                     IHNPKAFWAPYPLPSIALDDPAFVRPIPRNSWGGATQALTALRAPRWMEYYGKPADLA
                     WMMQRWIEAISRHIEFRQQMDPLTGDFTRIDPSGYSPAALVFFDYLWRLSGVRQHDDL
                     IEWNIRLPASSGRASFSAKIHNVSAELSYHGNHAQLRLAGKTIAEVFGTVRLLTTPAG
                     VLHSASGISNQEEHITIQISKGKPRVLRMEPNAHRAEL"
     gene            1707..3149
                     /locus_tag="VFA02_06945"
     CDS             1707..3149
                     /locus_tag="VFA02_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZU09622.1"
                     /translation="MSEFIEGRRAFLKSTVAAAVTAAVPSSAPAEARPSRKPNIIYYV
                     ADQMRWDFLGAQGENPTTLTPNLDAIAARGTRFATAVTNQPVCSPSRSVMLTGRYATE
                     TGVWSNARAIDPSLPTLASVLRQNGYTANFIGKWHLAPSSEQEGGGRGPVKPEFRAGF
                     LDYWEGANEYEWTTHPYEGTIWDGDGNPITFKDQYRVDFLTDRVVKFLQRKHERPFLL
                     FVSQLEPHFQNDVKHFVGPNGSKDKFANPFVPHDLNGKPGDWQGELPGYYGCIASIDQ
                     SVGRILKTLEEQGQLENTIFMFSSDHGCHFKTRNTEYKRSPHNASIRIPLIAQGPGFD
                     NAATVHEVVGNIHITPTLLDAVGVPIPESMKGKSLLPLAQNTSGRRQWKNRELVQISE
                     AMTARAIRTRDWTYSVVDPDANVQTDKFGKKYQDYLLYDQRADPYEAVNLSWRPEHRE
                     TIRELRQQLKEEMQYAGEPEAEFVSSRLFY"
     gene            complement(3143..4405)
                     /locus_tag="VFA02_06950"
     CDS             complement(3143..4405)
                     /locus_tag="VFA02_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HZU09623.1"
                     /translation="MHFSNWTIIFTWVISLVSILLMLLRPRGIAEYIWICLGALTLVL
                     AGALPLSDAAHAIYEGLDVYLFLAGMMILAELARHEGVFDWVADVAVHHSNGSPARLF
                     LLIYLAGTVVTIFLSNDATAVVLTPAVLAAVRRARVEPKPYLLACAMIANAASFVLPI
                     ANPANLVVFGKTLPPLAPWLRLFLLPSVFSIASTYFCLRLLSRHVLQGKMNSKADGIQ
                     LSPQGRLALFGICIAAATLVVTSGLGFPLGLPTCITGVFALLLISFRNGGAWKAVLRG
                     VSWSVVPLVAGLFMIVAALNRAGMLRLAQAGLAWLARVPDGLDKLVAAGVVAGLSNIM
                     NNLPVGLASGSALQPMHSAGALTHAVLIGVDLGPNLSVTGSLATILWLIALRRDDAEI
                     TAWEFLKTGMIVMPVSVALSVLSLHLVQ"
     gene            complement(4504..>5312)
                     /locus_tag="VFA02_06955"
     CDS             complement(4504..>5312)
                     /locus_tag="VFA02_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09624.1"
                     /translation="QVAWLKQAGRLSFDFNYTWSKSLGIINSTLDAFNVHGNYGILAI
                     DRPQVFNASYAFDLGSLYHGNFLLWKGVSNGWTISGINTFQSGSNLQSNSSQNLGLTI
                     YGKDANGNTEQLTSKTWFGTDANSILPIATCDPRSNLRSHQLLNLSCFSAPQIGQQGV
                     RQFHPYLNGPWYTDSDLSVYKTFAITERQHVQFRASAFNFMNHPLWGFSGNNLLTLKY
                     NTPDRGHTFTTDASTLGLASTSLWGVMNQKAAYSGAGYNRIVELSLKYTF"
BASE COUNT         1322 a         1596 c         1335 g         1059 t
ORIGIN      
        1 ccagttcaca acagatgacg ctcgcctgat gcaaacctac acggctgcgc tttctgtgct
       61 tcagcgtaac atcacacagt tgcatcctta ctccggcccg gtcttggtgg aagggagcgt
      121 ctatcccggc gtgtggctcg aatgcgcgcc acaggaaggc gaagtgtaca gcgcgatccg
      181 tccagatatc gcccgcaata accacatggt cttctttgct ctgcaaaaag aagatggaca
      241 gcttccgtgc agcgtaaaga tggactccat tggctttgga cagatccaaa tggttgtccc
      301 catcgccgcc accgcatggg agcttgcccg gaaaagcggc gacaacgagc tgctcgataa
      361 agcctatctt gcgtgcagcc gttgggacgc atggttgcgc cgctatcgaa acacacgcgg
      421 tacaggattg tgcgaaggct tctgtaccta tgacactggt cacgacaaca gcccgcgctg
      481 gaaaggcatt cccaatcgct gcccggatgc agacgcccgc aagtgtccgc ctgttccggg
      541 aatgccgcgt ctctgccctg atctttctgc ctctgtatat ggcggcagag ttgccctggc
      601 cgccatggca aaggctttag gcagacaaag cgaatccgac cgctggacag aggacgcaga
      661 atcaatccga aaacttatga tcgaaaaact ctattgtccg gaagatgctg cattttacga
      721 cctcgataca gaaggccatt tcattcgcat tcgcggagat gtgatttcgc gcgtcctcgg
      781 cgagcacgtc gtagactcca ggctctttga catcatctac cagaaacaga ttcacaaccc
      841 gaaggcattc tgggcaccat atccgctgcc atccattgct ttagatgacc ccgctttcgt
      901 gcgtcccatc ccgcgcaatt cctggggagg agccacacag gcgctcaccg ccctgcgtgc
      961 cccacgctgg atggaatatt acggcaaacc cgcagacctc gcctggatga tgcagcgctg
     1021 gattgaagcc atcagccgcc acatagaatt tcgccagcag atggacccgc tcaccgggga
     1081 ttttacccgg attgacccca gtggctattc gccggccgcg ctggttttct tcgactatct
     1141 ctggaggctt tcaggagtac ggcagcacga tgacctgatc gagtggaaca tacgccttcc
     1201 cgcctcttca gggcgtgcat cgttcagtgc caaaatacat aatgtaagcg cagaactcag
     1261 ttaccacggc aatcacgcgc agctgcgcct ggccggaaag accatcgccg aagtcttcgg
     1321 cactgtgcgt ctgctcacga cgcctgctgg tgttcttcat tccgcttcag gaatttctaa
     1381 ccaggaagaa cacatcacca tccaaatatc caaaggaaag ccacgcgtcc tcagaatgga
     1441 accaaacgct catagagcag aactctgaat gaaacgtgtg ctcgcatctc gaagagatgt
     1501 gaatttggat tctgacaaga acagaatcca agggcccggc ccgggagtct ggcccctttt
     1561 ttttgacaaa ctgttctacc tctcatatcc tctaaggcaa catgacaagg ctatttctcc
     1621 tcatctgtgg ctcctgttag ctttaaacct gctttcccta ccttttagct catgaaacgt
     1681 tattgccggg agacaagcgc atttccatgt cagaatttat cgaaggccgc cgcgcatttc
     1741 tgaagagcac cgtagcagct gcagtcacag ccgctgttcc atccagcgcg cctgctgaag
     1801 caaggccgtc gcgtaagcca aacatcatct attacgttgc cgaccagatg cgatgggact
     1861 ttctcggtgc gcagggcgaa aatccgacta ccctcacgcc aaatctcgac gcaatcgccg
     1921 ctcgcggaac acgctttgcc acagctgtca ccaatcagcc ggtctgctcc ccatcgcgct
     1981 ccgtcatgct gacaggacgg tatgctaccg aaaccggagt gtggagcaac gcccgcgcaa
     2041 ttgatccttc cttgccaaca ctcgcctccg ttctgcgcca gaacggctac accgcaaact
     2101 ttatcggcaa gtggcatctc gctccctcat cggagcagga aggcggcgga cgcggaccgg
     2161 tcaaacccga atttcgcgct ggctttctcg attactggga aggcgcaaac gagtacgaat
     2221 ggaccacaca tccctacgaa ggcaccatct gggacggcga cggaaacccc atcaccttca
     2281 aagaccagta ccgcgtggat ttcctcaccg accgcgtcgt caaattcctc cagcggaaac
     2341 atgaaaggcc ttttctgctt tttgtctctc aactggaacc acacttccag aatgacgtga
     2401 aacacttcgt cggcccgaat ggctcaaagg acaagtttgc aaacccattt gtgccccacg
     2461 acctgaatgg caaacccggc gactggcagg gcgagcttcc cggctactac ggttgcattg
     2521 cttccattga tcagtctgtg ggccgcattt tgaaaacact tgaagaacag ggccagcttg
     2581 aaaacaccat ctttatgttt tcgagcgacc acggatgcca ctttaagacc cgaaatacgg
     2641 aatacaagcg cagcccccac aatgcctcca tccgcatccc actcattgct cagggccccg
     2701 gttttgacaa tgcagccact gttcatgaag tcgtaggaaa catccacatt acgcccacgc
     2761 ttttagacgc agttggcgta ccaattcctg aaagcatgaa aggcaaaagc ctgcttcctc
     2821 tggcgcaaaa cacatccggg cgcaggcaat ggaagaaccg tgaactggtc cagataagcg
     2881 aagccatgac ggcacgcgcc atccgcacca gagactggac ttacagcgtc gttgacccgg
     2941 acgcaaacgt gcaaaccgat aaattcggta aaaaatatca ggactatctt ctctacgacc
     3001 aacgcgccga cccttacgaa gccgtcaacc tgagctggcg ccccgagcac agggagacaa
     3061 tacgggaact acggcaacaa ctcaaagaag aaatgcaata cgcaggagaa cccgaagcag
     3121 aatttgtctc ttcgcgactc ttctattgaa cgagatgcag gcttaagacc gacagcgcca
     3181 cagaaaccgg catgacaatc atgccggttt tcaaaaactc ccatgccgtg atctccgcat
     3241 cgtcgcgccg caacgcaatc aaccacagaa tcgtagccag tgacccggta acagaaaggt
     3301 tcggccccag atctacgcca atcagcacag catgcgtcag tgcgcccgcg ctatgcatcg
     3361 gctgtaacgc agatccgctc gcgagtccca ccggcagatt gttcatgatg ttcgacaaac
     3421 ccgccacaac tcctgccgca accaacttgt ccaaaccatc cgggacccgc gccagccagg
     3481 ccagtcctgc ttgtgcaagc cgcagcatgc ctgcgcgatt cagcgcggca acaatcataa
     3541 aaagccctgc taccagcggc accacgctcc aggaaactcc tcgcaacacc gccttccatg
     3601 ctccgccgtt gcgaaaggaa ataagcagca gcgcaaacac gcctgtgatg caggttggca
     3661 atcctaatgg aaaacccagt ccggatgtaa ccacaagtgt cgcggcggca atgcagattc
     3721 cgaacagggc cagccgtcct tgcggcgaaa gttgaatgcc atctgctttg gagttcatct
     3781 ttccctgaag cacatgacgc gaaagaagcc tcaggcagaa atacgtgctt gcaatcgaaa
     3841 atactgacgg cagcagaaac aggcgcagcc acggggccag cggcggcagc gttttcccaa
     3901 aaaccacaag gttcgccgga ttggcaatcg gcaacacaaa gctggccgca ttggcgatca
     3961 tcgcacaggc cagcagatac ggcttcggtt ctacacgtgc ccggcgcacg gcagccagca
     4021 ctgccggagt cagcacgacc gccgtagcat cattggaaag aaaaatagtc accactgttc
     4081 ctgcaagata aatcagcagg aacaaccgcg ccggcgatcc attggagtgg tgcacagcca
     4141 catcggccac ccagtcaaac acgccttcat gccgtgccag ctctgccaga atcatcattc
     4201 cggcaagaaa aagataaaca tccagccctt catagattgc gtgcgccgcg tcggacagcg
     4261 gcagcgcgcc cgccagcacc agcgtcagag cgcccaggca tatccatatg tactcggcaa
     4321 tgcccctggg ccggagcagc atcagaagaa tgctgaccag cgaaatgacc cacgtaaaga
     4381 taattgtcca gttggaaaag tgcatctggc ttcttggatt ctcttacact tcatcgcctg
     4441 ccgcaaaaga aaaggcgaag tggactgcaa tccacttcgc cagagtttcg ggagtataca
     4501 aattcagaac gtgtacttca gcgacagttc cacaatgcgg ttataacccg ctcctgaata
     4561 cgccgctttc tggttcatca cgccccacag gctggtggaa gcaagaccca gagtgctcgc
     4621 gtcggtcgtg aatgtgtgcc cgcggtctgg agtgttatat ttcagcgtca gcagattgtt
     4681 gccgctgaat ccccagagcg gatggttcat gaaattgaag gccgaagcgc ggaactgcac
     4741 atgctgacgc tcagtgatgg caaacgtctt gtaaacgctg aggtcagaat ccgtgtacca
     4801 cggcccattg aggtatgggt gaaactgccg cacgccctgc tggccaatct gcggcgcgct
     4861 gaagcaggaa aggttcagca actggtgcga acgcagattg ctccgcggat cgcacgtggc
     4921 aatcggcaga atcgagttcg cgtccgtgcc aaaccatgtc ttcgatgtca gttgctccgt
     4981 gtttccattt gcgtcttttc cgtaaatggt cagaccgagg ttctgcgatg aattcgactg
     5041 gagattgctg ccggactgga acgtgttaat gccggaaatt gtccagccat tggacacgcc
     5101 cttccacagc aggaaattgc cgtgatacag gctgcccaga tcgaacgcat acgaagcatt
     5161 gaagacctgc ggacggtcaa tcgccagaat gccatagttg ccgtgaacat tgaaagcatc
     5221 cagcgtgctg ttgataatgc ccagcgactt tgaccacgtg tagttgaagt caaaggaaag
     5281 gcgtcccgcc tgcttgagcc acgcaacctg ca
//
LOCUS       DASRBV010000182         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_111595, whole genome shotgun sequence.
ACCESSION   DASRBV010000182 DASRBV010000000
VERSION     DASRBV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111595"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /gene="recG"
                     /locus_tag="VFA02_06960"
     CDS             <1..2127
                     /gene="recG"
                     /locus_tag="VFA02_06960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZU09625.1"
                     /translation="DLLYHLPFRYEDRLHPRALNELQPGEMASVIAEVRGTALLRTRA
                     MPIFEMTVGQGLHSLKCIWFHGTYLKDKFRHGQMLALYGKVEASRSGRGFKMIQPQFE
                     VLPSDHDDDESRLLEVGRIVPVYETLGGNRLTSRWIRRIIFNLLRDLNGNIRETLPKA
                     LSERLQLPSRAQALHDVHFPPAGTPLSRLQASATAAHKRLIFEELFYLELGLELKRRK
                     FKERQGIAFETNDQVRSAIREVLPFHPTAAQKRALAEIVADMRRPFPMRRLLQGDVGS
                     GKTIVALQAMLVAIENGHQAALMAPTEILAAQHYLAAKKLLERASKRYRIVLLTGSLE
                     DGLKRLNRRRIYEGVADLVIGTHALIEEKVEFHKLGLIVVDEQHRFGVMQRFKLMKKP
                     TDAEPDVLVMTATPIPRTLALSLYGDLDLSVIDELPPGRTPIVTRKVSDERAEDVWKF
                     VREQVAQGHQAYVVYPVIEGAKDDQPELDFAPPEQEEERPAKKMSKTPLLFEKPTLKT
                     ATEMYESLRAGALAGLRLGLLHGRLDSDEKEIVMRRFQRGEIDVLISTTVIEVGVDVP
                     NATVMVIEHAERFGLAQLHQLRGRVGRGGAKSWCILMTGGKVSPEAEERLAAMVRTQN
                     GFELAELDLQLRGPGEFFGTRQAGLPDFRVANLLRDRKVLETAKEEAARFVRNPEGEA
                     SEEERKLVREQLREAWQRRYGLVEAG"
     gene            complement(2177..3112)
                     /gene="murQ"
                     /locus_tag="VFA02_06965"
     CDS             complement(2177..3112)
                     /gene="murQ"
                     /locus_tag="VFA02_06965"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZU09626.1"
                     /translation="MANGHATPVTNLHELITESPNEASQGFDTKSALEIARIINHEDA
                     KVAAAVKKALPEIALVIDAVARSLRDGGRLIYVGAGSSGRIAALDSCECPPYFSNSPQ
                     QIQYIMAGGPKALASPVEVNEDSEELGQRDIARRRPTRKDVVIGLSASGRTPYVVAAV
                     AYARARGAKTACITCNQNTPLAAAADIAIVAEVGPEVISGSTRMKAATAQKMITNMIT
                     TGAMTRLGYVYENLMVNVHMQNSKLVERGIGILMRACNVDRETAVRTIKSAGRSVPVA
                     LVMLKANVDKPEAVRRLARSDGNVRRAIEDTVMDL"
     gene            complement(3265..4362)
                     /locus_tag="VFA02_06970"
     CDS             complement(3265..4362)
                     /locus_tag="VFA02_06970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU09627.1"
                     /translation="MPLPLFHSGQREQFAQARSILEQAVANRAFPAAAYGVLYRGKII
                     ALDSVGRFTYDNGAETVLPETVFDLASVTKVLATTSMAMLLYDRGILDLETPLGEVLP
                     AFVIGRDPGSGKERVTLRMLLAHSSGLPAYARLFEQHTSANGLLRACLQMPLEASPGM
                     RVEYSDIGFILLGKALEVLAGDYLARYCEREIFAPLGVTNTFFCPPPQMRPQIPPTVN
                     DTEFRKRIIQGEVHDEHAYVLGGYAGHAGLFANVFDVLTFAGCILSEGLTADGHRLFR
                     PETVRLFATRQNPLRALGWDTPTPPSSSGKYFSPSSIGHLGYVGTSLWIDPERQLAVV
                     LLTNRTWPDRSNKAIQQVRPTFHDAIITSLP"
     gene            4413..5345
                     /locus_tag="VFA02_06975"
     CDS             4413..5345
                     /locus_tag="VFA02_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZU09628.1"
                     /translation="MNKPSLWIVLALCLLAFHPIHAQEDTALEAQLRALTALHHGKVA
                     IYAKDLNSGKTIAINADTPVPTASVIKLTVLFEALKQIQAGKAHFEDRVRLTKENQVE
                     GSGVLGFFDTPQTLTLKDILTMMIVVSDNTATNVAIDKLGLKNIDERIQWLGLKDTWL
                     YKKVAKPAVGPMPPDQKQFGLGKTTAREMAEVMERFDTCNLNAPGRTANSKGRDQKLC
                     DAAMHMLRNQFYRNSIPRYLETIDTTEGESKIANKTGALDHVRNDVGVVDAKNGPVIL
                     SEFTWDNQDTSWTPDNSAEVLMAKAAKIIVDRWQ"
     gene            5383..6165
                     /locus_tag="VFA02_06980"
     CDS             5383..6165
                     /locus_tag="VFA02_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09629.1"
                     /translation="MYRPFITGLLAVATCAFAQQQQPAAAATDAKTVVVPEGTKVLLE
                     LRSAINTKTAQPGDGVYLSSSFPVVVNGRVAIPVGVYVQGVIDQVTRPGRIKGRAAVR
                     MHFTSMIFPNGSVVTIPGVVNSLPGSGGPKVKKEGEIEQAGNKGKDAGTVAQDTMAGA
                     SLGTIGGAVSGSPGKGALIGALGGGAAGLITTLLTRGDEVVLPAGQGVEMVLQRPLTI
                     TQANLSGPDVAGQATPVAPAQQPLGKPKYKMLCPPGSLGCVD"
     gene            6174..>6580
                     /gene="lepB"
                     /locus_tag="VFA02_06985"
     CDS             6174..>6580
                     /gene="lepB"
                     /locus_tag="VFA02_06985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZU09630.1"
                     /translation="MSTENGQQIQKSTEAARSGFQLWVRDFIISVMASFFIITFLYQP
                     VKVEGTSMQPELRDQDRLFVNKFAYDFEKISRGDVVVFYYPRDPSKSYIKRVIGLPGD
                     NIQIRDGRVFVNGKLLGEPYVPRSYRDVRSMSE"
BASE COUNT         1445 a         1666 c         2125 g         1344 t
ORIGIN      
        1 gacctgctgt atcatctgcc gttccggtat gaggacaggc tgcatccgcg cgcgttgaac
       61 gaattacagc ccggcgagat ggccagcgtc attgccgagg tgcgtgggac tgcactcctg
      121 cgcacacggg cgatgcccat ctttgagatg acggtagggc aggggctgca ttcgctcaag
      181 tgcatctggt ttcatggaac gtacctcaag gacaagttca gacacgggca gatgctggcg
      241 ctttatggca aggtggaagc atcgcgctcc gggcgtggct tcaagatgat tcaaccgcag
      301 tttgaagtgc tcccatcaga ccacgatgac gatgagagcc ggttgctcga ggtggggcgc
      361 attgttccgg tgtatgaaac actgggcgga aaccggctta cctcgcgatg gatacggcga
      421 atcatcttca atctgctgag agatctcaat gggaacattc gggaaactct gccgaaggcc
      481 ctctcagagc ggctgcaact gccatcccgt gctcaggccc tgcacgatgt ccactttcct
      541 cccgcgggca ctccgctgtc ccggttgcag gcgtcggcaa cggcggcgca taagcggctc
      601 atctttgagg agctgttcta tctggaactg gggctggagt tgaagcggcg gaaatttaaa
      661 gaacggcagg gcattgcctt tgagacgaat gatcaggtcc ggagcgccat tcgcgaggtt
      721 ctgcccttcc atccaacagc ggcgcagaag cgcgccctgg cggagattgt cgccgatatg
      781 cggcggccct tccccatgcg gcggcttttg cagggagatg tcggctcagg gaaaaccatc
      841 gtcgcgctgc aggccatgct ggtggcgatt gaaaatggcc atcaggccgc gctgatggcg
      901 ccgacggaaa ttctggcggc acagcattat ctggcggcaa agaagctgct ggagcgggcg
      961 tcgaagcgct acagaatcgt cctgcttacc ggttcacttg aggacgggct gaagcggctg
     1021 aaccgccgac gtatttatga gggtgtggcc gacctggtca tcgggacgca cgcgttgatt
     1081 gaggaaaaag tcgagttcca caaacttggc ctgattgtcg tagatgaaca gcaccgtttt
     1141 ggggtcatgc agcgattcaa gttaatgaag aaaccaaccg atgcagagcc ggacgtgctg
     1201 gtgatgacgg cgacacccat tccacgcact ctggcgctgt ctctgtatgg cgacctggat
     1261 ctgagcgtga ttgatgagct tccgcccggg cgcacgccaa ttgtcacacg caaggtgtcc
     1321 gatgagcgcg cggaggacgt gtggaagttc gtgcgcgagc aggtggccca aggacatcag
     1381 gcatatgtgg tctatcccgt gattgaaggc gcgaaggacg accagccgga gctggacttt
     1441 gctcctcccg agcaggaaga agaaaggcca gcgaagaaaa tgtccaaaac accgctgctg
     1501 tttgagaagc ccaccctgaa gaccgcgaca gagatgtatg agtcgctgcg cgcgggtgcg
     1561 ctcgctggat tgcgcctggg gcttctgcat gggcggctcg attccgatga gaaggaaatt
     1621 gtgatgcggc gctttcaacg cggcgagatt gacgtcctga tctcaaccac ggtgattgaa
     1681 gtaggcgtgg atgttccgaa cgccacagtc atggtcattg aacacgccga acgctttgga
     1741 ttggcgcagt tgcaccagct tcgtgggcgt gttggccgtg gaggcgcaaa gtcctggtgc
     1801 atcctgatga cgggcggaaa ggtctctccg gaggccgagg agcggctggc agcgatggtc
     1861 cgcacgcaga acggttttga gctggctgaa ctggatttac aactgcgcgg gccgggagaa
     1921 tttttcggaa cacgacaggc gggactgccg gatttccgtg tggcaaatct gctgcgcgac
     1981 cgcaaagtac tggagacagc aaaagaagag gcagcgcggt ttgtgcggaa tcctgaaggc
     2041 gaagccagtg aagaggaacg aaagctggtc cgggaacagt tacgcgaagc atggcagaga
     2101 aggtatggac tggtagaggc cggataaaag aaaagcgccc aggcgggcgc aatcattcaa
     2161 gaaataacag ctttcgctat agatccatca cggtatcttc aatcgcacgg cgcacattgc
     2221 catcggagcg ggcgaggcgg cggacggcct ccggcttgtc cacgttggcc ttcagcataa
     2281 caagggcgac agggacgctg cgtccagcgg acttgatggt acgtactgcg gtttcgcggt
     2341 ctacattgca ggcgcgcatc agaatgccga tgccgcgctc gacgagtttg gagttctgca
     2401 tgtggacgtt gaccatcagg ttctcgtaga cgtagccgag gcgcgtcatc gcaccggtgg
     2461 tgatcatgtt tgtgatcatc ttctgtgcgg tggcggcctt catgcgggtg gagccggaga
     2521 tgacttcggg gccgacctca gccacgatgg cgatgtctgc ggctgcggcc aggggagtgt
     2581 tctggttgca ggtgatgcag gcggtctttg ctccgcgagc gcgggcgtag gcaacagcag
     2641 caacaacgta cggagtgcgt ccgctggcgg agaggccgat gacgacgtct ttgcgggtgg
     2701 gacgacggcg ggcaatgtcg cgttggccca gctcttctga gtcctcgttg acttccactg
     2761 gagaggcgag cgccttagga ccacccgcca taatgtattg gatctgctgc ggcgagttgg
     2821 agaagtaagg aggacattcg caggagtcga gcgcagcgat gcgtccgctg gagcctgcac
     2881 cgacgtaaat aagtcttccg ccatcgcgca ggctgcgggc aacggcatct ataacgagcg
     2941 cgatttccgg aagggccttt tttactgcgg ctgcaacttt tgcgtcttcg tgattgatga
     3001 tgcgcgcgat ttccagcgca gatttggtgt cgaagccctg cgatgcttca tttgggcttt
     3061 ctgtgatgag ttcgtgtagg ttggtcactg gcgttgcatg gccattggcc atgcgtggga
     3121 tttctgcttg tggaggtgtg gtgatggttg ccatggctgt actgtgtcct gcactcttgc
     3181 catcccttat tgtagctcta atggcaatgc cttgagcgca ttttccatgt tgcggcaaaa
     3241 atctcctttc ctgaaaaaga tttttcaggg aagagaagtg atgattgcat cgtgaaatgt
     3301 ggggcgcacc tgctggatgg ccttgttcga ccggtcgggc caggtgcggt ttgtaagaag
     3361 aacgacggcg agttggcgtt ccgggtctat ccagagagag gtgccaacgt agccgagatg
     3421 cccgatggaa gatggactga agtattttcc ggacgaggaa ggcggagtgg gagtgtccca
     3481 tcccagggca cgcagggggt tttgtctggt tgcgaagagg cgcacggtct caggcctgaa
     3541 gagccgatgc ccatctgctg taaggccttc ggagagaatg cagccggcga aggtcagaac
     3601 atcgaatacg ttggcaaaga gcccggcgtg accggcgtat ccaccgagca cataggcgtg
     3661 ttcgtcatgc acttcgccct ggatgatgcg tttgcggaat tctgtatcgt ttacggtagg
     3721 cggaatttgc gggcgcattt gcggcggcgg gcagaagaag gtgttcgtta ctccaagcgg
     3781 agcaaagatt tcgcgctcac aataacgggc cagataatct ccagcgagca cttcgagcgc
     3841 cttgccgagc aggatgaagc caatatcaga gtattcgaca cgcatgccgg gggacgcttc
     3901 gaggggcatc tgaaggcagg cgcgcagcag gccattggcg gaagtatgct gctcgaagag
     3961 tcgcgcatag gcgggcagtc cggaggaatg cgcgagcaac atgcgcaggg tcacgcgctc
     4021 ttttccactg cctgggtcgc gtccaatgac gaaggcgggg aggacttctc cgagcggcgt
     4081 ttcaagatcg agaatgccgc gatcataaag aagcatcgcc atcgaagtag tggcgaggac
     4141 tttggttacg ctggcgagat caaagacagt ttccgggagg accgtctcgg ccccgttgtc
     4201 ataagtgaag cggcctacgg agtcaagcgc gatgatcttg ccccggtaga gcacaccata
     4261 ggccgctgcc ggaaaggcgc ggttggccac ggcctgctca agaatggaac gcgcctgagc
     4321 aaactgctcg cgttgtccgg aatggaagag cggtaaaggc atagcaattc gatgcaggtg
     4381 aaagtatagt aagcgatttc aggagaaaac gaatgaacaa accgtctcta tggattgtgc
     4441 ttgccttatg tcttctcgct tttcacccga tacacgcgca ggaagacacc gcgcttgagg
     4501 cacagctccg cgcactgacg gccctgcatc atggcaaggt ggccatttac gccaaagacc
     4561 tgaacagcgg caagacaatt gccatcaatg cggacacgcc tgtaccgacc gcttccgtga
     4621 ttaagctgac ggtgttgttt gaggcactga aacagatcca ggcgggcaag gcacattttg
     4681 aagaccgggt aaggctgaca aaggaaaacc aggtggaagg gtctggcgtg ctcggttttt
     4741 tcgatacccc acagacgctt acgctgaaag atattctgac gatgatgatt gttgtcagcg
     4801 acaacacggc aaccaatgtg gccattgaca aactgggcct gaagaacatt gatgagcgca
     4861 tccaatggct gggcctgaag gacacctggc tttacaagaa ggttgccaaa cccgcggttg
     4921 gtccgatgcc tccggaccag aagcaatttg gcctgggcaa gacgacggct cgcgaaatgg
     4981 cggaggtaat ggagcggttt gatacctgta atctgaatgc tccgggacgc acggcaaact
     5041 ccaagggcag agaccaaaag ctatgcgatg cggccatgca catgctgcgc aaccagttct
     5101 atcgcaacag tatcccgcgt tatctggaga cgattgatac gacagaaggc gaatcgaaaa
     5161 ttgctaacaa aaccggggcg ctcgatcatg tccggaatga tgttggggtg gtagatgcaa
     5221 aaaatggacc agtcatcctt tcagaattta cctgggacaa ccaggacacg agctggacgc
     5281 cggacaattc tgccgaagtc ctgatggcga aggcggcaaa gatcatcgtt gaccgctggc
     5341 agtaatggcg cgtctacaat atagacaggg ccggagggcg ccatgtacag gccattcata
     5401 accggactgc tggccgttgc gacctgcgct tttgcgcagc aacagcagcc ggcggctgca
     5461 gctacagacg cgaagacagt tgtcgttcct gaaggaacaa aggtgcttct ggaactgcgc
     5521 tcggcgatca atacaaagac cgcgcagccg ggggatggcg tctatctttc atcgagcttt
     5581 ccagtggtag tgaatggacg ggtcgccatt cctgtaggag tgtatgtgca gggcgtaata
     5641 gaccaggtga cgcgtccggg acgcatcaag gggcgggcgg ctgtacggat gcactttact
     5701 tccatgatct tcccgaacgg gagcgttgtt acgattcctg gagtggtcaa cagcctgcct
     5761 ggttcgggcg gcccaaaggt gaagaaggaa ggtgaaattg aacaggccgg caataaggga
     5821 aaagacgccg ggaccgttgc tcaggacacg atggcgggcg cgagtctggg tacaattggc
     5881 ggcgcggtat caggcagtcc gggcaagggc gcactcattg gcgctctggg tggtggtgcg
     5941 gccggactga ttacgacact gctgacgcgc ggcgatgagg ttgtgcttcc agccggacag
     6001 ggagtggaaa tggttttgca gcgtccgctg acgattacgc aggcaaacct ttccgggccg
     6061 gacgttgcag gccaggccac gcccgttgct cctgcgcaac agccgctggg gaagcccaag
     6121 tataagatgc tgtgccctcc cggcagcctg ggatgcgttg attaaacgga cagatgagca
     6181 ctgagaacgg tcaacagatt cagaagagca cggaggccgc ccgcagcggt tttcagttgt
     6241 gggtgcgcga cttcatcatt tcggtgatgg catcgttctt tatcattacc tttctgtacc
     6301 agccggtaaa ggtcgaaggg acgagcatgc agccggagct gcgcgaccag gaccgcctgt
     6361 tcgtcaacaa atttgcctat gactttgaga aaatctcacg cggcgatgtg gtcgtcttct
     6421 attacccgcg cgatccctcg aagagttaca tcaagcgtgt gatcgggctg ccgggcgata
     6481 atattcagat tcgcgatggc cgcgtctttg tcaatggcaa gctgctggga gagccgtatg
     6541 tcccgaggag ttaccgcgat gtgcgctcga tgagcgagat
//
LOCUS       DASRBV010000183         9396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_725117, whole genome shotgun sequence.
ACCESSION   DASRBV010000183 DASRBV010000000
VERSION     DASRBV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725117"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFA02_06990"
     CDS             <1..448
                     /locus_tag="VFA02_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZU09631.1"
                     /translation="FGQKDAAQVAVLRKMVRDLNFPVQLVVCPIVREPDGLAMSSRNR
                     YLSPEERRQALVLSRSLKRIEDLAAQGERSSQKLTAAAREVLAEGPSVRIDYVAIVDP
                     DTLEDVPEVRHSALAAVAAFVGNTRLIDNTILGASASNNLSFRRPK"
     gene            complement(587..3184)
                     /gene="glnD"
                     /locus_tag="VFA02_06995"
     CDS             complement(587..3184)
                     /gene="glnD"
                     /locus_tag="VFA02_06995"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZU09632.1"
                     /translation="MTPEIQSLPRIRDAYQNDIADLRQTFERSGDGSASIRRRSTLVD
                     KVLRQLWTEIIAAHAEPFNITLVATGGFGRRELFPFSDVDVLFLCGNDGAERDFHQAI
                     RATIQAMWDTGLRASPSIRTWKECDRVDPDNLEFLVSLLDRRYLCGDYSLYRRFENDL
                     LPSLVLREWNTIVQNLAEIARARHAKYGNTIFHLEPNIKECPGGLRDYHLAQWLSLLF
                     SLQENKAWPKPTGNSFFTVHNDLESAFDFLAATRCFLHYRSGRDNNTLDWHAQDGAAA
                     QSIGLETRGSADPAYWMRTYYRNARTIFRRASLLMDDVPPPRRSFYKQFRRRRTPVPG
                     TDFVLEQGRLDLNSTGTVNDAEMVLRIFGQIAKHGYKLSQNAEDRIAEALPALAVQMP
                     EGPFLWNCLREVLLGPHAAHALRTMHALGLLELVIPEFHGIDALVIRDSYHRYTVDEH
                     TFLVIDNVHALRQPAHDWEKRFATLLPEIDRLDLFLLAILMHDTGKARRTGDHCTQSV
                     ELCESLFARLDFEPEERETIRRLIRNHLDMSSALRRDIFDPQSIRAFGEKVNSQQQLK
                     MLTLLTYADIKSVAPEALTPWKAENLWQLYIATSNFLDRSVDEERYHVESDTGTLNRI
                     LALVPDRSEELKTFLEGIPQRYLQTHLPDQIRSHFRMALALAEDPVQLAFRPLRQLHE
                     VTLITKDRPMLFAEMAGVLSAWGMNIVKADAFSNSAGIIVDTFQFTDSFQTLELNPTE
                     VDRFLKSIRDVISRQVPLETLLRARSHVNRHHQAKVKVETRLMFDNEASSHSTLLQVV
                     AQDTPGLLRELAKSFAEQQCNIEVALIDTEGEVAIDVFYLTSQGKKLELSQQRELGAS
                     LEKAIEELR"
     gene            complement(3313..3651)
                     /locus_tag="VFA02_07000"
     CDS             complement(3313..3651)
                     /locus_tag="VFA02_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZU09633.1"
                     /translation="MQKIEAVIQPSKLDAVKDALVEVGIEGMTILEARGHGRQKGHTE
                     FYRGREYTVDLLPKIKLETVVPDELVEKAVQAILSAARSGKIGDGKIFISKIDEAVRI
                     RNDERGPSAL"
     gene            complement(3702..5099)
                     /locus_tag="VFA02_07005"
     CDS             complement(3702..5099)
                     /locus_tag="VFA02_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZU09634.1"
                     /translation="MHRCRSIKFFLAITVLAGGLFSASAQAPASDQIAKLQAQVTAAQ
                     SGADNAWMLTSAALVLMMTGPGLALFYGGLVRKKNVLGTMMQSFAMMAIITVLWAFLT
                     YSLAFDSGTSFIGGLHHIFLKGVGLQPDPDYSATIPLQTYMIYQLMFAIITPALITGA
                     FAERMKFSAMALFLTLWSIVVYSPMAHMVWGKGGLLNASLGGKFPTLDFAGGTVVHIT
                     SGVSALVCALYLGKRLGYPREATPPHSVVLSFIGACLLWVGWFGFNAGSALNSGTLAT
                     SAFVNTHFGAAAAALGWMAAEWIRNGKPSALGGISGAVAGLVAITPAAGFVKPMSALV
                     IGVAAGVFCYFMVFKVKNWFGYDDSLDAFGVHGAGGTLGAVLTGLFASSAINPIFKDA
                     NGNTLPSGAVDGNWHQVLNQLVGIAIAWVISIVGTLILLKIVDLLIGLRVPREHELEG
                     LDLTQHGEEGYDFAS"
     gene            5364..5936
                     /locus_tag="VFA02_07010"
     CDS             5364..5936
                     /locus_tag="VFA02_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZU09635.1"
                     /translation="MSELARDRYLFGNLESSAKNQEKLREVSYGYEQPEGVFLTSLDS
                     AINWIRKNSIWPMTFGLACCAIEMMSMGASRFDVARFGAEVFRASPRQSDLMVIAGRV
                     SQKMAPVIRRLYEQMPEPKWVISMGACATSGGVFNNYALVQGVNQVIPVDVYVPGCPP
                     RPEQLIYAITLLQEKIQRERGSLKRALNLQ"
     gene            6296..7981
                     /locus_tag="VFA02_07015"
     CDS             6296..7981
                     /locus_tag="VFA02_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09636.1"
                     /translation="MYSRPLGSVGRILLVSCAVGLGASSLYGQNSNPQPAAPAERPVS
                     RVDVFTGYSYLAPHGTVNTPQPDGSVFSDRYSSIDYGAIGSVAYYFNRYFGGQVEYAN
                     HPDGRNDGAQTIQGGPIFRYPTSEGITPFVHALAGAVYLGGPNAEPFAAHPYTWGPAL
                     TAGGGLDYALPFLGGHVALRLFQADYEYFHVNYGPVVSYGGRANVNNARLSTGLVWKF
                     GNIVPPPPVQYSCSASPASVYPGDPVTITGTATNLNPKKTATYSWSGQGVTVKGDTAT
                     ANVDTTGLQPGNYTVTGHVSEGQKAGQFADCTASFTVKQYEPPTVSCSVNPSSVNPGD
                     TVTVTATGVSPQNRPLTYSYSASAGSISGTGNTATLSTTGAPAGTITVTANVADDKGQ
                     TASGTCTVTVNAPAPPAPKTQTLCSINFDRDKRRPTRVDNEAKACLDDVALNAQRSAD
                     AKVVVVGNETDQEKAEAAKTAKRKHPLSDFAAQRAVNTKDYLVKEKGIDASRIEVRTG
                     TAGSKEVENYLVPAGANFDSDVSGTTAVDESAVKAQPRKALATRHHRHTAKKK"
     gene            8180..>9396
                     /locus_tag="VFA02_07020"
     CDS             8180..>9396
                     /locus_tag="VFA02_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZU09637.1"
                     /translation="MKRKLLRFFYLGTFCFFFLHGNAQLQAQSHPWTAIGPDGGDARS
                     FAADPKTPQHIYLGTTTSLIYQSEDGGSTWKRIARIGKDDDLVVDSLVVDNSDPRTLL
                     AGVWQIDRPSGGIYISHDGGVTWTPSSDMQGQSVRALVQARSNPKIFVAGTLAGVFRT
                     EDRGQHWKQISPPGSGEIHEIESLAIDPYDPNTIYVGTWHLPWKTTDGGEHWHSIKQG
                     LIDDSDVFSIIIDPTRPTVIYTSACSGIYRSETAGELYRKVQGIPSTARRTRVLMQDP
                     VDRKTVYAGTTEGLYKTVDDGVNWTRMTGPDVIINDIYVDPSNPRHVLLATDRSGVLQ
                     SSDAGASFSASNAGFSQRQVTTILADAKHPGTIYAGVINDKTYGGVFVTEDNGSTWQQ
                     RSQGLNGRDVFTLA"
BASE COUNT         2014 a         2830 c         2601 g         1951 t
ORIGIN      
        1 cttcgggcaa aaggacgccg cgcaggtcgc cgtcctgcgc aaaatggtgc gtgatttgaa
       61 ttttcccgtg cagcttgtcg tctgccccat cgtgcgcgag ccggacggcc tcgccatgag
      121 ctcccgcaat cgctatttat cgccggaaga gcgcagacag gcgctcgtgc tctcgcgttc
      181 cctcaagcgc atcgaggacc tggccgcgca gggcgagcgt tcttcacaga agctgaccgc
      241 agccgcccgc gaggtcctcg ccgaagggcc ttccgtcagg atagattatg tcgcaatcgt
      301 agaccccgac accctcgaag acgtccccga ggtccgccac agtgctctcg cagcggtggc
      361 tgcatttgtg gggaacacgc gcctgattga caacacaatc ctgggcgcat cagcttcgaa
      421 taatctgtca ttccgacgac cgaagtgagg aggaatctta cggcattttc acagattctg
      481 tacactctat gcactgtgca gtgtcgccga ctccctgctg gttgcggcga ctgtagcttc
      541 gcggtatggt ccagactatc tgtgaaaacg ctagagcgat gtgaggttag cgtaattcct
      601 caatcgcttt ttccaatgag gcccccaact cgcgctgctg cgacagttcc agcttttttc
      661 cctgcgacgt caggtagaag acgtcaatcg ccacctcgcc ttccgtatca atcagggcga
      721 cttcaatatt gcattgctgt tcagcaaagc tcttcgccag ttcgcgcaga agaccgggcg
      781 tgtcctgcgc caccacctgt aaaagcgtgc tgtgcgacga ggcctcgtta tcaaacatca
      841 gccgcgtttc caccttcact ttcgcctgat gatgccggtt cacatggctg cgcgcgcgca
      901 gaagcgtttc cagtggcacc tggcgcgaaa tgacatcgcg aatgcttttc agaaagcggt
      961 ctacttccgt cgggttcagt tcgagcgtct gaaaggagtc cgtaaactga aatgtgtcta
     1021 cgataattcc ggcgctgttt gagaacgcgt cggccttcac aatgttcatg ccccaggccg
     1081 aaagcactcc cgccatctcg gcaaaaagca tcgggcggtc tttggtgatc agcgtgactt
     1141 catgcagttg ccgcagaggg cggaacgcaa gttgcacggg gtcttcggca agggccagcg
     1201 ccatacggaa gtgactgcgt atctggtcag gaaggtgcgt ctgcaaatat cgctgcggaa
     1261 tgccttcgag gaaggttttt aattcttcac tccggtccgg gaccagagca agaatgcggt
     1321 tgagtgtccc agtgtcgctc tccacgtgat agcgctcttc gtccacgctg cggtccagaa
     1381 aattcgacgt agcaatataa agctgccaca ggttctctgc cttccacggc gtcagcgctt
     1441 ccggcgcaac agacttgatg tcggcataag taagcagcgt gagcatcttg agctgctgct
     1501 ggctgttgac tttctcgcca aaggcgcgga tactctgcgg gtcaaaaatg tcgcgccgca
     1561 gagcagatga catgtcgaga tgattgcgaa tcaaccgacg tatggtttcg cgctcttccg
     1621 gctcaaagtc caggcgggca aaaaggctct cacacagttc cacgctctgc gtgcagtggt
     1681 cgcccgtgcg gcgcgctttt cccgtatcgt gcatcaggat ggcgagcaga aacaggtcga
     1741 ggcggtcaat ctccggaagc agcgtggcga agcgcttttc ccagtcgtgc gcaggctgcc
     1801 gcaacgcgtg aacattgtct atgacgagaa atgtgtgctc atccaccgta tagcgatggt
     1861 acgagtcgcg gatgaccaga gcgtcaatgc cgtggaattc cggaatgacc agttccagca
     1921 ggcccagcgc gtgcatcgtg cgcagcgcgt gcgcggcatg cggccccagc aggacctcgc
     1981 gcaggcagtt ccacaggaac gggccttccg gcatctgcac agccaatgcc ggcagcgctt
     2041 cggcgatgcg gtcttcggcg ttctggctca gcttgtatcc atgctttgca atctggccaa
     2101 agatgcgcaa caccatctcc gcatcgttga cagttcccgt gctgtttaaa tccagccggc
     2161 cctgttcgag cacgaagtcc gttcccggca ccggagtgcg ccgccggcgg aactgcttat
     2221 aaaacgagcg ccgcggcggc ggcacatcat ccatcagcag agaagcgcgg cggaagatcg
     2281 tgcgggcatt gcggtagtag gtgcgcatcc agtatgcggg gtcggcgctg ccacgggtct
     2341 ccagcccgat gctctgcgct gcggccccgt cctgggcgtg ccagtcgaga gtgttgttgt
     2401 cgcgcccgct gcggtagtga agaaaacatc gcgtcgccgc cagaaaatca aaggccgact
     2461 caaggtcgtt gtgaacggta aagaacgaat tgcctgtagg cttgggccac gccttgtttt
     2521 cctgcagaga aaacagcagc gacagccact gcgccagatg ataatcgcgc agaccacccg
     2581 gacactcttt gatgttcggc tcaagatgga agatcgtgtt gccgtatttg gcatggcgcg
     2641 cgcgagcaat ctcggccagg ttctgcacga ttgtgttcca ctcgcgcaga acaagcgatg
     2701 gcagcagatc attttcaaag cgccggtaaa gtgaatagtc tccgcacagg tagcgccggt
     2761 cgagcagcga aaccagaaat tccagattgt caggatcaac ccggtcgcac tctttccagg
     2821 tgcgaatcga ggggctggcc cgcaggccgg tgtcccacat ggcctgaatc gtggcgcgaa
     2881 tcgcctgatg gaaatcgcgc tccgcgccgt cgttcccgca caggaagagc acatccacat
     2941 ctgagaaagg aaaaagctcc ctgcgcccaa aaccacccgt cgcgaccaga gtgatattaa
     3001 acggctcggc gtgggccgca atgatctcgg tccagagctg gcgcagaact ttgtccacca
     3061 gcgttgagcg gcggcggatg gaggccgacc catctccgct gcgctcaaac gtctggcgca
     3121 ggtctgctat gtcgttttga tatgcgtccc gtatgcgcgg gagggattgg atctctggcg
     3181 tcatgcggtc ttcaattcag gtgcggggat ggaagcggct tccttattta tcaagggagc
     3241 cgcattttct ggctttcgtc aatctcaatc agaattgtca tcctgaaaga agcgcgcagg
     3301 cgaaagcctg ttttacagcg ccgaaggccc gcgctcgtca ttacgaatgc gaacagcttc
     3361 gtcaatcttc gagataaaaa tcttgccatc gccgatcttc ccgcttcgcg ccgcggaaag
     3421 aatggcctgc acggccttct ccaccagctc gtcggggacc acggtttcca gcttgatctt
     3481 cggcagcaaa tctaccgtgt actcacgtcc gcggtaaaac tcggtatggc ccttctgccg
     3541 gccatggccg cgggcctcaa gaatcgtcat gccctcaatg cccacttcca ccagggcgtc
     3601 tttgactgca tctagcttcg acggttgaat gaccgcttca atcttctgca tagtgtgtcg
     3661 tctcagaggt tacagggtaa caggttctac cattctatgg cttacgaggc gaaatcgtag
     3721 ccttcttcac cgtgctgcgt cagatcaagg ccctcaagtt catgctcgcg tggcacgcgc
     3781 agaccaatca acaggtccac aatcttcagc agaatcagcg ttcccacaat tgagatgacc
     3841 caggcaatcg caatgcccac cagctggttc agcacctggt gccagttgcc gtccaccgct
     3901 ccggaaggca gcgtgttgcc gttcgcgtct ttgaaaatgg ggttgatggc gctcgacgcg
     3961 aaaagtcccg tcaggaccgc gcccagtgtg ccgcccgctc cgtggacgcc aaaggcgtca
     4021 agtgagtcgt cataaccaaa ccagttcttc accttgaaga ccatgaagta gcagaagacc
     4081 cccgccgcca cgccgatcac cagcgccgac atcggcttta caaaacctgc cgcgggagta
     4141 attgccacca gtcctgccac cgctccggaa ataccgccaa gcgcgctcgg cttgccattc
     4201 cggatccact ccgccgccat ccagcccaaa gcagccgcgg ctgcgccaaa gtgcgtgttg
     4261 acgaaagcgc tcgtggccag cgtccctgag ttcagcgcgc ttccggcgtt gaatccgaac
     4321 cagcccaccc acagaagaca ggccccgata aagctcagca cgacggagtg cggcggcgtc
     4381 gcttctctgg gatagcccag ccgtttgccc agatacaggg cgcagaccag cgctgacact
     4441 cctgacgtta tgtgcacgac cgtgccgcct gcaaaatcca gtgtcgggaa ttttccgccc
     4501 agggatgcat tcagcagccc gcccttgccc cagaccatat gcgccatcgg tgagtaaaca
     4561 acaatggacc acagcgtaag aaacagcgcc atcgcactga acttcatgcg ttcggcaaag
     4621 gccccggtga tgagcgccgg ggtaatgatg gcgaacatca actggtaaat catgtaagtc
     4681 tgcagcggga tggtcgccga gtaatcgggg tccggctgaa gtccgacgcc cttcaggaaa
     4741 atatggtgca gcccgccgat aaagctcgtg ccggaatcaa aggcgagact atacgtcagg
     4801 aaggcccaga gcaccgtaat gatggccatc atggcaaaac tctgcatcat ggtgcccagc
     4861 acgttctttt tccgtaccag accgccatag aaaagcgcca gaccgggccc cgtcatcatc
     4921 agaacaagtg cggcgctggt gagcatccag gcattgtccg ctccggactg cgcagccgtc
     4981 acctgcgcct gcagttttgc gatctggtca gaggcaggag cctgcgcact ggcagaaaac
     5041 aacccgcccg ccaacacagt tatggccaaa aaaaatttta tgctgcgaca acgatgcata
     5101 acaccccatc gaacagattt gagatagacc ctgcccgccc gcatgactgc aaaccagcca
     5161 aaaggactgc ggcgcaaggc aaacaggaaa tgtcttgtgt gaaattccag aatatgttgc
     5221 agctactttt acatatttct gaaaaatcac aaacagcaaa tttttatacg gagaataaag
     5281 aaaatttctt gggcgccgga aatattttca ccgttggccc gggctgcctc gcccggccgg
     5341 tcgtgcagcg ttatagtaga aatatgagcg aattagcgcg cgacaggtac ttgttcggca
     5401 atctggagag cagtgccaag aaccaggaga aattgcggga agtcagttat ggttatgagc
     5461 agccagaagg cgtctttctg acctcgctgg attcggccat caactggatc cgcaagaact
     5521 ccatctggcc catgaccttc gggctggcct gctgtgccat tgagatgatg tccatgggcg
     5581 catcgcgctt tgacgttgcc cgcttcggcg cagaggtctt tcgcgcctca ccgcgccagt
     5641 ctgacctgat ggtcatcgcc gggcgtgttt cgcagaagat ggccccggtc atccgccgcc
     5701 tgtatgagca gatgcccgag ccgaaatggg tcatttccat gggcgcatgc gccacttccg
     5761 gaggcgtttt caacaactac gccctggtgc agggggtgaa tcaggtaatt cctgtagatg
     5821 tgtacgtacc cggctgcccg ccccgtccgg agcagttgat ctacgccatc accctgcttc
     5881 aggaaaagat ccagcgtgag cgtggcagct tgaaacgcgc gttgaacttg caataacctg
     5941 aggggttact ggcactgctt cgtcagtccc tcagtaagtt atgatatttc aataggttaa
     6001 taatacctgg ccatgtctct ccagcagggg ggacggaagg ccagcttccc ggctctgctg
     6061 ttccgggatt ttcttccggg aatgtttgac tgctgttaac ttcaggggtg ctcttcagaa
     6121 ttcagtgcat tctgaggcaa cttattgaag aatttatgtt ctaacctagt gaaacgtgtg
     6181 gtaagttttt ataccgtcgt agttttctta cgcggtttct gcccggcttg aatcttaagc
     6241 cgaaaatttt atctgtgttg tgactttcaa agctatggtt gcggaggata gacgcatgta
     6301 ttctcgtccg ttaggttccg tgggccgaat tctgcttgtc agttgcgccg taggactcgg
     6361 tgcttcgagt ctttacgggc agaattcgaa tcctcaaccg gctgctcctg ctgagcggcc
     6421 cgtttcccgt gtagatgtat ttaccggcta ttcttatctc gcgccgcatg gcacggtcaa
     6481 cacgccacag cctgatggct ccgtgttcag tgaccgttat tcgtccattg attacggcgc
     6541 tattggcagc gttgcatact acttcaatcg ctattttggc gggcaggtcg agtatgcaaa
     6601 ccacccagat ggccggaatg acggcgccca gacgatccag ggcggcccga tcttccgcta
     6661 tccaacttct gaggggatca ccccatttgt tcatgctctg gctggagcag tatatctcgg
     6721 tggacccaat gctgagccgt ttgctgctca cccttatacc tggggcccgg cgctgaccgc
     6781 tggtggcgga cttgattatg ccctgccctt tctgggcggc cacgtcgcac tccgcctctt
     6841 ccaggctgat tacgagtact tccacgtgaa ctatgggcca gttgtttcgt atggcggtcg
     6901 cgccaacgtc aacaacgccc gcctcagcac cggtctggtc tggaagttcg gcaacatcgt
     6961 tcctccgccg cctgtccagt acagctgctc ggccagcccg gcgtctgtct atcctggcga
     7021 cccggtcacg attaccggca ctgccaccaa cctgaacccc aagaagaccg cgacctatag
     7081 ctggtctggt cagggcgtga cggtgaaggg tgacacagcc accgccaacg tggacaccac
     7141 tggtctccag cccggtaact acaccgttac cggacacgtc agcgaaggcc agaaggccgg
     7201 ccagttcgcc gactgcaccg cgagcttcac tgtgaagcag tatgagccgc cgacagtcag
     7261 ctgctcggtc aacccgtcct ccgtcaatcc gggtgacacg gtcaccgtta ccgctactgg
     7321 cgtaagcccg cagaaccgtc cgctgaccta cagctacagc gcatcggctg gttccatcag
     7381 cggcacaggc aacacggcta cgctttccac caccggcgct ccggctggca ccatcaccgt
     7441 gaccgccaat gttgccgacg acaagggtca gacagcatcc ggcacctgca cggtgacggt
     7501 caatgctccg gcgcctccgg caccgaagac gcagacgctt tgctcgatca acttcgatcg
     7561 tgacaagcgc cgtccaaccc gcgtagacaa cgaagccaag gcctgcctcg acgatgtggc
     7621 gctcaacgca cagcgttcgg ccgatgccaa ggtggttgtc gtgggcaacg aaaccgacca
     7681 ggagaaggca gaagctgcca agaccgcgaa gcgcaagcac ccgctgtctg acttcgccgc
     7741 gcagcgcgct gtcaatacga aggactacct ggtgaaggag aagggcattg atgcaagccg
     7801 catcgaagtc cgcactggca cagcaggctc gaaggaagtg gaaaactacc tcgtgcccgc
     7861 aggcgccaac ttcgactccg atgtgtcagg caccaccgct gtggatgagt cagcagtcaa
     7921 ggcccagcca cgcaaggccc tggctaccag gcaccaccgt cacaccgcca aaaagaagta
     7981 agtgcggtgg aatgcagcac gaaggccagc cggagttccg gctggccttt tcttctcagt
     8041 agagcgagct ttgccgggtt gcatcattcc ggtccggcag gtgggcttgt agctctgcgt
     8101 acagtacttc ttttttccgg cacactgttt tgcatcggga aatcaaacca gaatcggagt
     8161 aaagttgtaa ttggaacata tgaagcgtaa gctactgcgg tttttctacc ttggcacctt
     8221 ttgtttcttc tttcttcatg gaaacgccca gcttcaggcc cagtcccatc cctggacggc
     8281 gattggtccc gatggcggag acgcgcgtag tttcgcagcc gaccccaaaa ctcctcagca
     8341 catttatctg ggcaccacca ccagcctgat ctatcaatct gaagacggtg gaagcacctg
     8401 gaagcgcatt gccagaatcg gcaaagatga cgatctggtc gtagacagcc tggtcgtgga
     8461 caactccgac cccaggaccc ttcttgctgg cgtctggcag atagaccgcc cttccggcgg
     8521 catctacatc agccacgatg gcggcgttac ctggacgccc tcttctgaca tgcagggcca
     8581 gtctgtgcga gctctcgtcc aggcacgctc gaatccaaaa attttcgtcg ctggaacgct
     8641 cgccggggtc ttccgtacag aagacagagg acagcactgg aagcagatca gccctcctgg
     8701 cagcggggaa atccacgaaa tcgagtccct cgccattgat ccctatgatc caaacaccat
     8761 ctacgtcggc acatggcacc ttccctggaa gaccaccgac ggcggcgagc actggcacag
     8821 catcaaacag ggtctgattg acgactccga cgtcttctct atcattattg acccgacgcg
     8881 gcccacagtg atttacacca gcgcctgctc cggaatctac cgcagcgaaa ctgccggcga
     8941 gctttatcgt aaggtgcagg gcatcccatc cactgcccgc cgtacccgcg tgctcatgca
     9001 ggacccggtc gaccgcaaaa ccgtctacgc agggacgacc gagggccttt ataagaccgt
     9061 ggatgacggc gtgaactgga cgcgcatgac cggccctgat gtcatcatta atgacattta
     9121 tgtagaccca tccaatcccc ggcacgtcct tcttgccacc gaccgcagcg gcgtgctgca
     9181 aagcagcgac gccggcgcga gcttctctgc atcgaatgca gggttttcgc agcgccaggt
     9241 gaccaccatc cttgccgatg caaagcatcc ggggaccatc tatgccggag tcatcaacga
     9301 caagacctat ggcggcgtct ttgtaacaga ggacaacggc tcgacctggc agcagcgcag
     9361 ccagggcctc aatggccgcg atgtcttcac cctggc
//
LOCUS       DASRBV010000184        15991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_675219, whole genome shotgun sequence.
ACCESSION   DASRBV010000184 DASRBV010000000
VERSION     DASRBV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675219"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1452)
                     /gene="gatA"
                     /locus_tag="VFA02_07025"
     CDS             complement(52..1452)
                     /gene="gatA"
                     /locus_tag="VFA02_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZU09638.1"
                     /translation="MESGSHKAAELVEAAYEQIRARDPQIHAYLSLNPERAYEQARRI
                     DEIAAKGAPLPPLAGVPVGIKDVLTIEGLPATAGSKILEGYIAPYTATSVRRLEDAGA
                     IILGKINCDEFAMGSSNENSAYGPVHNPHALDRVPGGSSGGSAAAVAAGMAVATLGTD
                     TGGSIRQPASFCGVVGILPTYGRVSRYGLIAFASSLDRVGPFAANVRDAAITLGVIAG
                     HDPMDATSSPLPVPDYTADLDKGVTGLKLGVPAEYFAEGLDQEVRAAIEKTIDQLHAA
                     GAEIRPISLPHTRYAIPTYYVIATAEASANLARFDGVRYGFRAKDANTLSAMYRRSRD
                     LGFGPEVKRRIMLGTYALSAGYYDAYYKKAQQVRRLLTDDFLKAFAEVDAILTPTAPT
                     PAFKLGEKTDDPVSMYLADIYTVTASLAGICGVSVPCGTSSEGLPIGLQILGRHFHEA
                     IALRVAQAVESLQSES"
     gene            complement(1509..1817)
                     /gene="gatC"
                     /locus_tag="VFA02_07030"
     CDS             complement(1509..1817)
                     /gene="gatC"
                     /locus_tag="VFA02_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZU09639.1"
                     /translation="MSVSLEDVRHVAELANLELTAEEEGRMLRDLNAILAHVAQMNEL
                     DTANVEPMAQVSEVIGQAQTETVLRSDEVRASLDRACVMATAPETDGTFFKVPKVIER
                     "
     gene            complement(1906..2640)
                     /locus_tag="VFA02_07035"
     CDS             complement(1906..2640)
                     /locus_tag="VFA02_07035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZU09640.1"
                     /translation="MATESDMMDSGGAMRIHYLQHVIFEGPAAIEDWARQKGHTVTGS
                     HLYRGDPLPGVSAFDLLVMMGGPMSVNDEHEHPWLLPEKQLVRKAVQHGRAVFGVCLG
                     AQLIASALGASVYQAPEREIGWFPVRRVTGERIGALLPQRFTPLHWHGETFDLPAGAV
                     RLAETDAVPNQAFQFGDNVVGLQFHMEATPQSVEMLVENAGQEIQEGQPFQQPPQIIV
                     AQAPEASAAVRPVLWKLLDHLVGAAK"
     gene            complement(2645..4552)
                     /locus_tag="VFA02_07040"
     CDS             complement(2645..4552)
                     /locus_tag="VFA02_07040"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU09641.1"
                     /translation="MQPKAPAPPQQGAGEKTPPVTPPSGHGLEPADLEAFFDGILPLQ
                     LERSDIAGASVLVMKDGKVLLLKGYGYADLKTKKPVDPNATMFRLASISKLFTWTAVM
                     QLEEQGKLDIDADVNKYLDFQIRPAFGKPITLRNLMTHTGGFEEADRDLIVVHPKKDI
                     SVREYLVENQPKRLFPPGEVPAYSNYGVGLASYIVQRVSGEPFEQYVQEHIFAPLGMT
                     HSTFYQPPPKELEPYVSVGYHGNTEKPSVGFEVVHPAGAGALSSSAADMGRFAMAFLN
                     GGELDGKRILKPETVAAMWTPQFRASDQMPPMCMGFYQMWRNGLHWIGHEGDLVAFHS
                     LFFVEPKERLVLFVSYNSAGGGDKPRPEIINLFSDRYFPSNEKQTFITVPRNELQEIA
                     GTYLTTRREDSTKFRLQNLLSQQRATVDKDGVLRIEDRDDLRGHNIKWKPAGKDLWQE
                     IEGQRRLFAIRDAQGKIVRLASDFPGIQLQRVPWYQNARLVLGIVCAALAVLLAAVVA
                     TLLRLWRRVFLRKRGLPAPQPGTRWLPFSGKAAAWIWSGLLLGIWYFLSGDEMPPTSA
                     WDKYFYLVNVITVVAMLFSVFAIISGLRIWKAAALRTITRVKFSLVALACFVLSIFAI
                     QWHLIGPATRF"
     gene            complement(4702..5481)
                     /locus_tag="VFA02_07045"
     CDS             complement(4702..5481)
                     /locus_tag="VFA02_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU09642.1"
                     /translation="MARQLYTARLRQKLLLSDPAQCYHLEFRIDDLENFEFTPGQFVS
                     CVAVDKNGKTQTRAYSIASAPRANEFDLCVNRVEGGFFSNLLCDLEPGQTVQFHGPHG
                     LFTLRQPLTDSIFIATGTGIAPMRGFVQWLFPENGEDRSEGRPIWLVYGTRYPTEIYY
                     QKYFEKIAAERRNFHYLSTLSRPKEDWQGLRGYVQEHVAKIVIERASTNHSGPVVTAA
                     EPGGFDIHAYICGLNDMVSANRDKLTELGWQKKQIIFERYD"
     gene            5566..8010
                     /locus_tag="VFA02_07050"
     CDS             5566..8010
                     /locus_tag="VFA02_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09643.1"
                     /translation="MPRYRLASGILFAITFFCAYAQTRVTLTVIDETGLPVPGAQVTI
                     QWPGGPTLQLQTDYAGRCVFTLRKNAAYAASVVKPGYFQTDSANIDPQLGNVQLVLSH
                     EQIVRTQVNVTASPVGIDPEQTSDKSTMNTPEIVNVPYPTSRDIRSLLPFNPGVVQDA
                     SGQVHIAGSETYATLDMLDGFDIRSPVSGMLAMRVSADAVRSIDIESTRYPVEFGRST
                     GGVVAFYTGMGDNKFRFNATNFIPSFHDINGIRFDKYVPRITFSGPIQRDHAWFFDGL
                     ETEYDSIYIKELPSGANTNNLIRGSNLIRAQVDLTPANILSGGFLFNDYHSPYDGISA
                     LTPQQSTTKRDTVAWLPYVRDQHSFHGGALLETGFGVVRFRDGYEPRGTLPYDLTPEL
                     SHGSYFESAVSHSRREEANAVLYLPPRSWFGRHDLKTGIDLDHIAFTGNYNRAPVNYL
                     REDGTLLRQSTFPALPPFTRHNVEAGAYVQDRWLMHSGLLFEPGVRFDWDEVLRRPEF
                     SPRFALVYSPSGTEAKTKISAGAGLYYEHTQLEYLQRALAGIRYDTYYAADGTTPVGG
                     PLLTSFAARPSALHQTRALNWSLGIEQKLPWQIYGGVNFLQKRIVNGWVYQNQSAPGA
                     LSGTYLLTDARRDHYNAFEIEARKNFANGYTLFGSYTRSSARTNAAVDYVPSVSLLGP
                     QQHGPLAWDAPDRVVSWGWLPFPLPWFRHNWDFVYTMQWQTGFPYTSINANRQVIGAP
                     GSRRFPDYVNFSPGLEWRFHFHGAYFGLRGVLENATGSQNPAIVNNVVDSPAYGTFSQ
                     PMGRALTGRIRLISEK"
     gene            8184..9122
                     /locus_tag="VFA02_07055"
     CDS             8184..9122
                     /locus_tag="VFA02_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZU09644.1"
                     /translation="MQRREFLAKAGAFAASCALAQPPFFAAETKLRPMDIGVLIRIGD
                     NPEEPVARVKELGLNNCFLSLDRYIGKFTPQLADQIGSALHKYGITATTAEVVGPGRL
                     VWDFEDGPSTIGLVPRESRAERLDALKQTSDFAKLLGVPQIQTHCGFIPENPRDPLYP
                     ETVQAIREITMHCANNGQHFLMETGQETPTTMSRAIKDVDHPNLGVGLDTANLILYGK
                     ANPVDAVDIIGPHVRSIHAKDGKWPTDPMKLGQEVLIGTGLVDFEKVFSKLHKLGYTG
                     AITIEREIEGAQQMEDIRKEKVYLQRIIEKIQSGNS"
     gene            9195..10145
                     /locus_tag="VFA02_07060"
     CDS             9195..10145
                     /locus_tag="VFA02_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HZU09645.1"
                     /translation="MPLSNDPNAVELAREVLKSFDKVAGGIHPGFRPGHAKGILLTGT
                     FTPSAQARTLTRAPHILRTSTPVTVRLSDFAGIPNVPDNDPNGASPRGCAIRFHLAEH
                     VHTDIVAHSVDSFPARTAEDLIGFLNAIAATGPDSPHPNPVEQYLSAHPEALAFIQIP
                     KPIPTSFAHESFFAVSAFKFTNAEGKSQYGRYRVLPEAGNEYLTAEAAAAQSPNFLFD
                     EVRQRVPRTPVKFRIVVQLAADGDITNDATVRWPEDRPLADFGEITLTGFVENDAKEQ
                     QHIIFDPIPRVDGIEASDDPLFQPRADVYLMSGRRRRSAN"
     gene            10165..11427
                     /locus_tag="VFA02_07065"
     CDS             10165..11427
                     /locus_tag="VFA02_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU09646.1"
                     /translation="MDRRQFLAGSAAASGLLLLKPKTAFGYEANSAVRYALLGCGNRG
                     TSVATSFAKNTSARVVALGDLFQDKLDAGKAHFDQINQSLGYSGPDPRLMFRGYKAYE
                     ELAASPHIDAIQISTPPYFHVQHLEAAVKGGKHAYCEKPVGVDIAQTNQALEIAKHVD
                     NKVSVDVGFQIRSAPPFVEIVRRIHSGELGKIASVEAWYNAPPSTYPDFPGASSDELR
                     IRRWLWDRVLSGDILVEQNIHVIDICNWALQGHPVKAIAKGGRSILTHPGDCYDNYQV
                     VFTYPDDVRVSFSSTQFCPAHWFDVSERFFGEKGIAEAPYSGPLRIISDNPWTWAGST
                     AQPTGSFAANGAFTDNLAQADSEKDKGFIESITSGNFHNQIAAGVESARSCMLGRMAA
                     YQKREVTWDELLKHGEKYELGINMSQFS"
     gene            complement(11461..11823)
                     /locus_tag="VFA02_07070"
     CDS             complement(11461..11823)
                     /locus_tag="VFA02_07070"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HZU09647.1"
                     /translation="MSLLDNVKQMVSGQAAESGEEAGLLGHVMDMVNNPATGGLQGFI
                     QQFHDKGLGGVVSSWIGNEENQAISAQQIEQVVGQERINAIASRLGIPPEEASAKLAQ
                     FLPSIIDRLTPGGKVEQA"
     gene            complement(11852..12187)
                     /locus_tag="VFA02_07075"
     CDS             complement(11852..12187)
                     /locus_tag="VFA02_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZU09648.1"
                     /translation="MKRKYSWTITVALAVALLFGANMSFAQSAAKTSTKSSATAAAAQ
                     TQSADLVDLNTASLDQLKALPGIGDAYAQKIVDGRPYAKKTDLVRKKIIPAATYRKIA
                     DKVIAKQSK"
     gene            complement(12218..12772)
                     /locus_tag="VFA02_07080"
     CDS             complement(12218..12772)
                     /locus_tag="VFA02_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09649.1"
                     /translation="MKLQLKRHISSLVLAAFFFGLAVPCALHAQSTQDQSASSTSSTT
                     KTKKKKSKTDASASTTSSTDTTSDQSATASTSASKHKKKSTSADTSATAMPASAPASA
                     PASASTMSASSSAPAASTHKAKASTSSSVQAQTPPSPGMVWVNTDSGVYHKAGSRWYG
                     KTKQGKWMTEQDAVKAGYKPASKE"
     gene            12970..14193
                     /locus_tag="VFA02_07085"
     CDS             12970..14193
                     /locus_tag="VFA02_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZU09650.1"
                     /translation="MRSSPTIFPIFGLSLFLAVSTGAQQQASPVRVFESPEPAASQTQ
                     STTAPATVQVRQGVQLIEAKAPDAQALSSRKGSYSFKVKSGDWIDTGVIVAPGDHLDF
                     TANGTITLADNRSSSPDGVARGWKDLLRQFPLNTANAGALIGRISSNDASVPFLIGAG
                     RQMDVTTSGHLYLNTNLSSDLSGDGSYSVKLKISKTQAAVTPASSVNLGNLLSPALFN
                     NIPRTVQDEQGDPGDMVNFAIIGTKDQVQKAFQAAGWTAVDKTTQDAILHGLLATLSH
                     EAYVEMPMSTLYLFGRPQDLSFARADPIAVAAVRHHLRVWQTTETVDGKPLWVGSATH
                     DNGFEKDQRNGKVTHHIDPNIDAERDFIQQSFAAAGMLAGAAYVTPADPLKESHTATG
                     GSFHSDGRIVVMMLR"
     gene            14235..>15991
                     /locus_tag="VFA02_07090"
     CDS             14235..>15991
                     /locus_tag="VFA02_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU09651.1"
                     /translation="MRTFDLLFGRPLATSEERAEQIGPAAGIPIFGLDALSSAAYGPE
                     AALTLLLPLGLAGVHYIVPVSFAIIALLVIVYFSYRQTIEAYPQGGGSYTVATENLGN
                     RAGLLAAAALMIDYILTAAVGISAGVGALVSAAPKLQPHTLALCLAILVLLTVVNLRG
                     VRDTGVAFMVPTCAFLLTLLLVIVLGGFQALTHGGHPQPIVAPPKLPPPTTTLTLWLL
                     LKVFSSGCTAMTGVEAVSNGVMAFREPRTKHAKLSLTIIIALLIVLLAGIALLCRAYN
                     IGATDPGAPGYESVLSQLTAAVAGKGIFYYATIGSILLVLALSANTAFADFPRLARAI
                     ALRDYLPHVFILRGRRLLYSHGVIALAFFTAALLVLFGGVTDRLIPLYAIGAFLAFTL
                     SQSGMVVHWRRQGNATGRMIVNGIGAVATGITVLVVLVAKFAEGAWVTALLVPLLILV
                     MKSVHRHYQRVQDETTEPGALAVHGLRPPRVILPMDKWNRISEKGLRFALMMSPEVEV
                     VHVDCGDEQDSICEAWHEKVLKPIQAAGLPVPELVTIKSPYRFFLQPLVEHVLAEEKK
                     YGDRQIAVLVPELVVQHWW"
BASE COUNT         3340 a         4923 c         4365 g         3363 t
ORIGIN      
        1 ttacggcgcg ggtagctttt cgacaggtgc tcgacagcga caagggccac gttacgattc
       61 gctttgcaga gactcgaccg cctgcgccac ccgcagcgcg atggcttcat ggaagtgcct
      121 gcccagaatc tgcaacccaa tcggaagccc ttctgaagat gttccgcagg gcactgagac
      181 tccgcagatt cccgcaaggc tcgccgtgac ggtatagatg tccgccagat acattgagac
      241 cggatcatcc gtcttctcgc ccagtttgaa tgcaggtgtg ggggccgttg gcgtcagaat
      301 agcgtccact tctgcaaatg ctttcagaaa atcgtccgtg agcagacgcc gcacctgctg
      361 cgccttcttg taataggcat cgtagtagcc tgcgctgagg gcgtaggtgc cgagcatgat
      421 gcgccgcttg acctccggcc caaaaccgag gtcccgcgag cggcggtaca tggccgagag
      481 tgtgttcgcg tctttggcgc ggaagccata acgcacgcca tcaaagcgcg caaggttggc
      541 gctggcctca gcggtcgcaa tcacataata cgtcgggatg gcatagcgcg tgtgcggcag
      601 cgagatgggc ctgatctcgg ccccggcagc gtgaagctga tcaatcgtct tctcgatcgc
      661 ggcccggact tcctggtcga gcccttcggc gaagtattcg gcgggaactc cgagcttcag
      721 tccggtcacg cctttgtcca gatcagcggt gtagtccggc acgggcagcg gagacgaggt
      781 cgcgtccatc ggatcgtgcc ccgcaatcac gcccagcgta atggcagcat cgcgcacatt
      841 cgcggcaaac ggcccaacgc ggtccagcga ggaagcgaag gcaatcaaac catagcggga
      901 gacgcgcccg taggtcggca gaatgcccac cactccgcag aacgaagcag gctggcgaat
      961 cgagccgccg gtgtccgtgc ccagcgtagc cacagccatg cccgccgcca cagctgccgc
     1021 cgagccgccg ctggagccgc ccggcacgcg gtcgagcgca tgaggattgt gcaccgggcc
     1081 ataggccgaa ttttcattcg acgaccccat ggcgaactca tcgcaattga tcttgcccag
     1141 gatgatggcg cctgcatctt ccagcctgcg gacggaagta gcagtatagg gcgcgatata
     1201 tccttccaga atcttcgacc cagccgtggc cggaagacct tcgatggtca ggacgtcctt
     1261 aatcccaacc ggaacacccg cgagcggagg cagcggcgcg ccctttgccg ctatctcgtc
     1321 aatgcggcgg gcctgctcgt aagcgcgctc cggattgagc gaaagatagg catggatctg
     1381 cgggtcgcgc gcgcgaatct gctcataggc tgcctcgacc agctcagcgg ccttatggct
     1441 gcccgactct atggcgcggc ggacttccgc aatcgaaagc ggcctggata ctgcggtttc
     1501 cactgtcatc agcgttcaat cactttcggt actttgaaga atgttccatc ggtttccggc
     1561 gctgtagcca tcacgcacgc gcggtcaagc gacgcccgca cctcatcgct gcgcagcacg
     1621 gtttccgtct gcgcctggcc aatcacctcg ctgacctgcg ccatgggttc gacgtttgcg
     1681 gtgtccagct cattcatctg cgccacatgc gcaagaatgg cgttcaggtc gcgcaacatg
     1741 cggccctctt cttcggcagt caactcaaga ttggcaagtt cggccacgtg gcgtacgtct
     1801 tctaaggaaa cgctcattgg gaaatacctt gaagggccag aaggaccctc tcctgagtat
     1861 agccgggata cggtgtgctt ccgtattttg ccggcccctt agagatcact tcgccgcacc
     1921 aacgagatgg tccagcagtt tccacaaaac aggacgcacc gccgcagatg cttccggcgc
     1981 ttgcgccacg attatttgtg gcggttgctg aaagggctgg ccttcctgga tttcctgccc
     2041 tgcgttctcc accagcatct cgacgctttg cggagtggct tccatatgga actgcaggcc
     2101 caccacgttg tcgccaaact gaaaggcctg gttcgggaca gcgtctgtct cagccagacg
     2161 caccgcgcct gcgggcagat cgaatgtttc cccgtgccag tgcagcgggg taaagcgctg
     2221 gggcagcagc gcgcctatgc gctccccggt cacacggcgc acaggaaacc agccgatctc
     2281 tcgttccggt gcctgataca cgcttgcacc cagagcgctg gcaatcaact gcgcgccaag
     2341 gcagacgccg aaaacggccc tgccatgctg gaccgccttc cggactaact gcttttccgg
     2401 caggagccag ggatgttcat gctcgtcatt gacgctcatc ggcccgccca tcatgaccag
     2461 caggtcgaag gcggaaacgc cgggcagagg gtctccacga taaaggtgcg agcctgttac
     2521 ggtatgcccc ttctggcgcg cccagtcctc aatggccgcc gggccttcaa atatcacgtg
     2581 ctgcaggtaa tggatgcgca tcgctccgcc tgaatccatc atgtcagatt cagtcgccat
     2641 gcctctagaa ccgcgttgcc ggaccgatca gatgccactg aatggcgaag atgctcagaa
     2701 caaagcaggc cagggccacg agtgaaaact tcaccctggt gatggttcgc agggccgccg
     2761 ctttccagat gcgaagcccg gagatgatgg cgaatacact gaacaacatc gcaactaccg
     2821 tgatgacatt gaccagataa aagtatttgt cccacgcgga tgttggcggc atctcgtccc
     2881 ctgagaggaa gtaccagata cccagcagga gccccgacca tatccatgcc gcagctttgc
     2941 ccgaaaatgg cagccaccgg gtcccgggct ggggcgcggg caggccgcgt tttcgcaaaa
     3001 acactctgcg ccacaggcga agcagagtag caactaccgc cgcgagcagc accgcaaggg
     3061 cagcgcacac aatgcccaga actagcctgg cattctgata ccaggggaca cgctgcaact
     3121 ggataccagg gaaatcggag gccagccgca caatctttcc ctgagcatcc cgaatggcaa
     3181 acagccgccg ctgtccttcg atttcctgcc agaggtcttt gccggctggc ttccatttga
     3241 tgttgtgtcc gcgcaaatca tcgcggtctt ctatgcgcag cacgccgtct ttgtccaccg
     3301 ttgcgcgttg ctgcgaaagc aggttctgga gccggaattt cgtgctgtct tccctccggg
     3361 tcgtcaggta ggtcccggca atctcctgca attcattgcg cggaacagtg atgaacgtct
     3421 gcttctcgtt actgggaaaa tagcggtcag aaaacagatt gatgatctct gggcgcggct
     3481 tgtctccccc gcccgccgag ttataggaga caaaaagcac cagccgctcc tttggctcca
     3541 caaagaacag gctgtggaac gccaccaggt ctccttcatg tccgatccag tgcagaccat
     3601 tgcgccacat ctggtaaaag cccatgcaca tgggcggcat ctggtcactg gcacggaatt
     3661 gcggcgtcca catggcggca accgtctctg gcttcagaat gcgcttgcca tccagttcgc
     3721 cgccattcag aaaagccatg gcgaagcggc ccatgtccgc ggcgctggag gaaagcgcgc
     3781 ctgctcccgc cggatgcacc acctcaaaac ccacggacgg cttctctgtg tttccgtgat
     3841 aacccacgga cacatatggt tccagctctt ttggcggagg ctggtagaac gtcgagtgcg
     3901 tcatcccaag cggcgcaaag atgtgctcct gcacatactg ctcgaatggc tccccgctga
     3961 cgcgctgcac gatatagctc gccagcccga cgccgtaatt cgagtacgcc gggacctcgc
     4021 ccggaggaaa aagccgcttc ggctgatttt cgaccaggta ctcgcgcact gagatgtcct
     4081 ttttcggatg gacaacgatc aggtcgcggt cggcctcctc aaagcccccg gtgtgggtca
     4141 tcagatttcg caacgtaatg ggcttgccga aagcaggacg tatctggaaa tcaagatatt
     4201 tgtttacgtc ggcatctatg tcgagctttc cctgctcttc aagctgcatg accgccgtcc
     4261 aggtaaacaa cttggaaatg gatgccagcc ggaacatcgt cgcattcggg tctaccggtt
     4321 ttttggtctt cagatcggca tagccgtagc ccttcaacag aagcaccttg ccgtccttca
     4381 tcaccagcac agaagcgcct gcaatgtccg agcgctccag ttggagcggg agaattccgt
     4441 cgaagaaggc ctcaaggtcg gccggttcca gtccgtgccc gctgggtgga gtcaccggcg
     4501 gggtcttttc acctgcaccc tgctgtggag gcgcaggcgc tttgggctgc atggagggag
     4561 cctgagtgac aaatggcaaa agaaaggcaa gcggaagaac agacatcccg aaaaatcgca
     4621 tgcattgctc cggattgctg aatgcgcacc attgtaacaa gcgcctttcc tatgcccttg
     4681 gcgctgagca gcaacgcaga ctcagtcgta tcgctcaaaa ataatctgct tcttctgcca
     4741 gcccagctcc gtaagcttgt cgcgattggc ggaaaccata tcattcagcc cgcagatata
     4801 cgcgtgaata tcgaacccgc ccggctcagc cgcagtgacc accgggccgg agtggttggt
     4861 gctggcgcgc tcgatcacta tctttgccac atgctcctgc acgtatccgc gcagcccctg
     4921 ccagtcctct ttaggacggc tcagggttga gaggtagtga aagttcctgc gctcggcagc
     4981 aatcttttca aaatatttct ggtagtaaat ctccgttgga taccgcgtgc catagaccag
     5041 ccagatgggc cgcccctcgc tgcggtcttc gccgttttcc ggaaaaagcc actgcacaaa
     5101 gccgcgcatc ggcgcaatac cggtcccggt cgcgataaag atagagtccg tcagcggctg
     5161 gcgtagcgta aacaggccat gcgggccgtg gaattgcacc gtctgccccg gctcaaggtc
     5221 gcacaacagg ttggagaaga acccgccttc tacacggttc acgcacaagt caaattcatt
     5281 tgcgcgcgga gcagaagcga tggaataagc gcgcgtctgc gtcttgccgt tcttgtccac
     5341 agcaacgcag gaaacgaact gtcccggagt aaactcgaag ttctctagat catcaatcct
     5401 gaattcaagg tgatagcact gcgccgggtc agagagtaag agcttctgcc gcaggcgcgc
     5461 cgtataaagt tgtcttgcca aggtttcgct tccttaaaga agatttgcgt gcatcaaaga
     5521 ttcatgtaag cttaagtcta ttttagctag ccttaactta ttctcatgcc gcgttatcgt
     5581 ctggcaagtg gaatactctt cgcgatcact ttcttctgtg catacgcaca gacacgtgta
     5641 acgcttacag tcatagatga aaccgggctt ccggtccctg gcgcgcaggt caccattcaa
     5701 tggccgggtg gcccaacact ccagttgcaa accgactacg ccggacggtg cgtctttacc
     5761 ctccggaaaa acgccgctta tgcagccagc gtcgtcaagc caggctactt ccagacagac
     5821 agcgcaaaca ttgacccgca gctcggcaat gtgcaattgg tcctctcgca tgagcagatc
     5881 gtgcgcacgc aggtgaacgt aaccgcctct cccgttggga ttgacccgga acagacctcc
     5941 gacaaaagca cgatgaacac gcccgagatc gtgaatgtgc cctatcccac ctcgcgcgac
     6001 atccgtagcc ttctgccctt caaccccggc gttgtgcagg acgcatccgg ccaggtgcat
     6061 atcgcaggct cggagaccta cgccacactc gacatgctcg acggcttcga cattcgttcg
     6121 cccgtcagcg gaatgctcgc catgcgcgta agcgcagacg ccgtgcgctc catagacata
     6181 gaaagcacgc gctatcccgt cgagtttgga cgcagcacag gcggcgtcgt ggccttctac
     6241 accggaatgg gcgacaacaa gtttcgcttc aatgccacca acttcatccc ttcctttcac
     6301 gacatcaacg gtattcgttt cgataaatat gtcccgcgca tcaccttctc cgggcccatc
     6361 caacgcgacc acgcctggtt cttcgatggc ctggagaccg agtacgacag catctacatc
     6421 aaggagctgc cctcaggcgc aaacacaaac aacctgatac gcggcagcaa tctcatcagg
     6481 gcacaggtag acctcacgcc tgccaatatt ctttccggcg ggttcctgtt caacgattac
     6541 cactcgccct atgacggcat ctccgcgctc actccccaac agagcacgac aaaacgcgac
     6601 acggtagcgt ggctcccata tgtgcgtgac cagcacagct tccatggcgg cgcattgctt
     6661 gagactggct tcggcgtcgt ccgcttccgt gatggatacg agcctcgcgg aacgctcccc
     6721 tatgatctga cgcccgagct ttcccacggc agttactttg agagcgctgt ttcgcattcg
     6781 cgccgcgaag aagccaacgc agtgctgtac cttccaccgc gctcctggtt cggacgccat
     6841 gacctgaaga caggcataga cctcgaccac atcgcattta ccggcaatta caaccgcgcc
     6901 cctgtgaatt atctacgcga ggacggcacg ctcctgcggc agagcacttt tccggccttg
     6961 cctcccttca cgcgccataa cgttgaggct ggagcgtatg tacaggaccg ctggctcatg
     7021 cactccggcc tgctgtttga gccgggcgtg cgctttgact gggacgaagt cctccgccgg
     7081 cctgaatttt cgccgcggtt cgcgctggtc tattcgccat ccggaacgga agcaaagacg
     7141 aaaatctccg ccggagcagg gctctattac gagcacacac agcttgagta tctgcaacgc
     7201 gccctggccg gcatccgtta tgacacctac tacgccgcgg acgggaccac ccccgtgggc
     7261 ggcccgctgc ttaccagttt tgcagctcgt ccttccgcac tgcaccagac acgcgccctg
     7321 aactggagcc tcggcatcga gcaaaagctt ccctggcaga tatatggtgg agtgaacttc
     7381 cttcagaaac gcatcgtcaa cggctgggtc taccagaacc agtccgcacc cggagccttg
     7441 tccggcacgt atcttctgac cgacgcgcga cgcgaccact acaacgcctt tgaaattgag
     7501 gcgcgcaaaa actttgccaa tggatacacg ctgttcggct cttatacccg ctcctcggcg
     7561 cgcacaaacg cagccgtgga ttacgtcccg tctgtttccc tgcttggccc gcagcaacac
     7621 ggcccactgg catgggacgc accggaccgc gtcgtttcct ggggatggct gccatttcct
     7681 ctgccgtggt tcaggcacaa ctgggacttt gtctacacca tgcaatggca gaccggattc
     7741 ccgtatacgt ccatcaacgc aaaccgccag gtcatcggcg cacccggctc cagacgcttc
     7801 cctgattatg taaacttcag ccctggtctc gaatggcgct tccactttca cggggcctac
     7861 ttcggcctgc gtggagtgct cgaaaacgca acaggcagcc agaatcctgc gattgtcaac
     7921 aatgttgtgg actcacccgc gtacggcacc ttcagccaac ccatgggccg cgcactgact
     7981 gggcggatcc ggctgatcag tgaaaaatga actttctcaa acacatcaac agcctcattt
     8041 atgcggatgc tgcctgtgca acagccaatt ccagatgaca aagaacgagc cacgccttgc
     8101 ctggaacacg gcccgcggca aaacttcgcc atcgcaatat ggtacccttc cgttttggaa
     8161 tcgttgccac gggagggcct tccatgcaaa gaagagaatt tcttgccaaa gcaggagcgt
     8221 ttgccgcaag ctgcgcgctc gcgcagcccc cgttctttgc tgccgaaacc aaactcaggc
     8281 ccatggacat cggcgtactc atccgcatcg gcgacaatcc tgaggagcct gtcgcgcgcg
     8341 tcaaagaact cggcctcaac aattgctttc tctcgctcga ccgctacatc ggcaagttca
     8401 ccccccaact tgcagaccag atcggcagcg cgctccacaa atatggcatc accgccacta
     8461 cggcagaagt cgtcggcccg ggccgcctcg tctgggattt tgaagatggc ccgtccacga
     8521 tcggccttgt cccgcgcgaa agccgcgccg aacgactcga cgctctcaag cagacctccg
     8581 atttcgccaa actcctcggc gttccgcaaa tccagacgca ctgcggattc atccccgaaa
     8641 acccgcgcga cccgctttat cctgaaaccg tccaggccat ccgcgaaatt acgatgcact
     8701 gcgccaacaa cggccagcat ttcctgatgg agaccgggca ggagaccccg acgacgatgt
     8761 cacgcgccat caaggacgtg gaccatccca acctcggcgt cgggctcgat accgccaacc
     8821 tcatcctcta cggtaaagcg aatccggtgg atgcagtgga catcatcggc cctcacgtcc
     8881 gcagcattca cgcgaaagat ggaaaatggc ctacggatcc gatgaagctc ggccaggaag
     8941 tgctgatcgg cacaggcctc gtggactttg aaaaagtctt cagcaagctg cacaagctgg
     9001 gatacacagg cgccattacc attgagcgcg aaatcgaagg cgcgcagcag atggaagaca
     9061 ttcgcaaaga aaaggtctat ctccagcgca tcatcgagaa aattcaaagc ggcaattctt
     9121 aagcgaaatt tatagcagga cgcaggaacg aatttcagtt ttgcagctaa agtagtaaga
     9181 cgaaggagaa acccatgcct ctgtccaatg acccgaatgc tgtagagctt gcccgtgaag
     9241 tgttgaagag tttcgataaa gtagctggtg gcattcatcc gggttttcgt cccggacacg
     9301 ccaagggaat tctgctgacc ggcacattta cgccttctgc tcaggcccgc acactcacgc
     9361 gtgcgcctca tatcttgcgt acatctactc cggtgaccgt ccgcctgtca gactttgcag
     9421 gaattcccaa tgtcccggac aatgacccga atggagccag tccgcgagga tgcgccatcc
     9481 gctttcacct ggcggaacat gttcacacgg acatcgttgc gcattcggta gattccttcc
     9541 cggcacgcac ggccgaagac ctcatcggtt tcctgaatgc tatagctgca accggtcctg
     9601 atagtccgca cccaaacccc gtggagcaat acctcagcgc acatcctgag gcgctggcat
     9661 ttatacagat tccaaagccg attccaacca gctttgcaca cgaatccttc tttgctgttt
     9721 cggcgtttaa gtttacaaat gccgaaggca aaagccagta tggacgctat cgcgtgctcc
     9781 cggaggcagg caacgaatat ctcacagcag aagccgcggc tgcgcaatcg ccgaattttc
     9841 tttttgatga ggtccggcag cgcgttccac gaacacccgt aaagttcagg attgttgtgc
     9901 agctggccgc ggatggtgac attacgaatg atgcgactgt gcgatggcca gaggaccggc
     9961 ctctggcgga cttcggtgaa atcacattga ctggatttgt cgaaaacgat gcaaaagagc
    10021 aacagcacat catcttcgac ccaattcctc gcgtggatgg catcgaggct tcagacgatc
    10081 cgcttttcca gccgcgcgcc gatgtgtact taatgagcgg gcgcaggcgt cgttctgcaa
    10141 attgaaaaaa cacagggaaa tttaatggat agaagacaat ttctcgctgg atccgccgca
    10201 gcctccgggc ttctcctgct gaaaccgaag accgccttcg gatacgaggc caactccgcc
    10261 gtgcgctatg cgctgctcgg atgcggcaac cgcggcacgt ctgtggccac ttcctttgca
    10321 aagaacacca gcgcgcgcgt tgtcgcactc ggcgatctct ttcaggacaa gctcgatgcg
    10381 ggcaaggcgc actttgacca gatcaaccaa tcgctcggct actctggccc ggaccccagg
    10441 ctgatgttcc gcggttacaa ggcctatgag gagctggccg cctctccgca tattgacgcc
    10501 atccagatat ctacaccgcc ctacttccac gtgcagcacc ttgaagctgc cgtcaaagga
    10561 ggcaagcacg cctactgcga aaaacccgtt ggcgtagaca tcgcgcagac aaatcaggcg
    10621 ctcgaaatcg caaagcacgt agacaataaa gtcagtgtgg acgtcggctt ccagatacgc
    10681 agcgcgcctc cgtttgtcga gatcgtgcgc cgcatccact cgggcgagct gggcaaaatc
    10741 gccagcgtcg aagcctggta taacgccccg ccgtccacct atcccgactt ccccggagcg
    10801 tcatccgacg agcttcgcat ccgccgctgg ctctgggacc gcgtgctctc tggagacatc
    10861 ctcgtcgaac agaacatcca cgtgattgac atctgcaact gggccttgca gggacatccc
    10921 gtcaaggcca tcgccaaagg cggacgcagc atccttacgc atccgggcga ctgctatgac
    10981 aactatcagg tggtcttcac ctaccccgat gatgtgcgcg tcagcttctc ctcgacgcag
    11041 ttctgcccgg cacactggtt cgatgtatcc gagcgcttct tcggagaaaa aggcatcgcc
    11101 gaagcgccct actccggccc gctgcgcatc atcagcgaca atccctggac atgggcagga
    11161 agcaccgcgc agcccaccgg ctccttcgcc gccaacggag cattcactga caacctcgcc
    11221 caggccgatt ctgaaaaaga caagggcttc attgaaagca ttacgtcagg caacttccac
    11281 aaccagatcg ccgcaggcgt tgaaagcgcg cgaagctgca tgctgggccg catggctgcc
    11341 taccagaagc gcgaagtcac ctgggacgag ttgctgaagc acggcgaaaa gtatgaactg
    11401 ggcatcaaca tgagtcagtt cagttagggc ctgttgaccc tctcattcaa tgtctcagtt
    11461 ctatgcctgt tctacttttc cgccgggtgt cagcctgtca atgatggaag gcaggaattg
    11521 ggccagtttg gcggaggcct cctcgggagg aatgccgagg cgcgaggcga tggcgtttat
    11581 cctctcctgc ccgacaacct gctcgatctg ctgcgccgaa atggcctggt tctcttcatt
    11641 gccgatccag gaactgacca cgccgcccag ccccttgtca tggaactgct ggatgaaacc
    11701 ctgcaaccct ccggttgcag gattgttcac catgtccatg acgtggccca gcagccccgc
    11761 ttcttcgccg ctctcggccg cctgaccgct gaccatttgc tttacattgt ccagaagaga
    11821 catacacgcc tccattgcct ctgtgtggga actactttga ctgtttcgcg atcaccttgt
    11881 cggcaatctt cctgtaagtt gccgcaggaa tgatcttctt gcgcaccagg tcggtctttt
    11941 tcgcatacgg acgcccgtct acgattttct gggcatacgc atcgccgatg ccgggcagcg
    12001 ccttcaactg gtccaggctg gccgtgttca ggtccaccaa atcagcagat tgcgtctgcg
    12061 ccgctgcggc cgttgccgat gacttcgtgc tggttttggc tgccgattgc gcaaaagaca
    12121 tattcgcgcc gaagagtagc gcgaccgcca gcgccacggt tatggtccat gaatatttcc
    12181 gtttcattcc tgttcgatct cctttgcgaa ataacaacta ctctttggaa gctggtttat
    12241 atccggcctt cacggcgtcc tgttcggtca tccatttacc ctgcttggtt tttccatacc
    12301 agcgggagcc ggctttgtga tacacgccag agtcggtatt gacccacacc atcccgggtg
    12361 aaggaggcgt ctgggcctgc actgagctgc tcgtggaagc ctttgctttg tgtgtggaag
    12421 cagccggcgc agacgaggat gcgctcattg tggatgcgct tgccggagcg ctggccggag
    12481 cacttgccgg catggccgtc gcggatgtat ccgcagatgt gctcttcttc ttgtgtttgg
    12541 atgcgctggt ggaagctgta gcgctctggt ctgaagtggt gtcagtgctg ctcgtggtgc
    12601 ttgctgaagc gtcagtctta ctctttttct tcttcgtctt cgttgtagat gaagttgagg
    12661 acgcgctctg gtcttgcgtg ctttgcgcat ggagggcgca gggtacagcc agcccgaaga
    12721 aaaatgcggc cagaacaaga gatgagatat gccgtttaag ctgcagtttc attcagcctc
    12781 cttttgctga ttgctgactt ctgcatgtta gcttcagagc aggccccgtc atgtgacaaa
    12841 tctatgaata agccactctt acgctccggg cgttcatagt ctgccgctgg agggtaagtt
    12901 gtcaaggaac ccccttggcg gcagcaacga ctttctcctt tccttctgcg tccataatgg
    12961 tataaggcca tgcgatcctc tcccacgata ttcccaatct tcggtctttc cttgtttctg
    13021 gccgtcagca ctggagcgca gcagcaggct tcgcccgttc gcgtttttga gtcgccggaa
    13081 ccggcagctt cgcagacgca aagcacgacg gccccggcca ccgtgcaggt gcgccaggga
    13141 gtgcaactga ttgaggcaaa agcacctgac gctcaggcgc tctcttcgcg caaaggctct
    13201 tattccttca aagtgaaatc cggtgactgg atagataccg gcgttatagt ggcccccggc
    13261 gaccaccttg atttcactgc gaacggcacg atcactcttg ctgataaccg ttcttcttca
    13321 cccgacggcg ttgcgcgcgg ctggaaagac ctgctcaggc aattcccgct caatacggca
    13381 aatgccggcg cgctgattgg ccgcatcagc agcaatgacg cctcggtgcc gttccttatc
    13441 ggcgctggca ggcagatgga cgtgaccaca agcggccatc tgtacctgaa cacgaatctg
    13501 agcagcgacc tctccggcga cggaagctac tcggtcaaac tgaaaatctc caaaacgcag
    13561 gccgcagtaa ctccggcaag ttcggtcaat ctgggcaatc tgctctcgcc cgctttgttc
    13621 aacaatattc cgcgtacggt gcaggatgag cagggggacc cgggcgacat ggtgaatttt
    13681 gccatcatcg gaacgaaaga ccaggtgcaa aaagccttcc aggcggctgg atggacggcc
    13741 gtggacaaga ctacacaaga cgccattctg catgggctgc tggccacgct ctcgcatgaa
    13801 gcctatgtag aaatgccgat gagcaccctg tatctttttg gacggccaca ggacctttcc
    13861 ttcgcgcgcg ccgatcctat cgccgtggct gcggtgcgtc atcacctgcg cgtctggcag
    13921 accaccgaaa ccgtggatgg caagcctctc tgggttggat ctgccacgca tgacaatggc
    13981 tttgaaaaag accagcgcaa cgggaaagtg acccaccaca ttgatccgaa tattgatgcg
    14041 gaacgcgact tcatccagca aagctttgcc gccgccggca tgcttgctgg tgccgcttac
    14101 gttacacccg cggacccgct gaaagaatcg cacacggcaa ccggaggaag cttccattcc
    14161 gatggacgca tcgttgtcat gatgctgcgt taatgccaac acgataggat ggttgcggct
    14221 gtcccgtcat cactatgcga actttcgacc ttctttttgg ccgtccgtta gctacatcag
    14281 aagagcgcgc cgagcagatt ggccctgccg caggcattcc cattttcggg ctggatgcgc
    14341 tcagctccgc cgcatatggc ccggaagccg cgctcacatt gcttcttccg cttgggctgg
    14401 caggcgtgca ttacattgtg ccggtcagct ttgcgattat cgccctgctg gtcattgttt
    14461 atttctctta ccggcaaacc attgaggcgt atccgcaagg cggcggctcg tatactgtcg
    14521 caaccgaaaa tcttgggaat agagcgggac tgctggctgc tgccgccctg atgattgact
    14581 acattctgac ggcggccgta ggaatctcag ccggagtagg ggcgctcgta tcggccgcgc
    14641 cgaagctgca gccgcacacg ctggcgctgt gtcttgcaat cctggtcctg ctgactgtcg
    14701 tcaatctgcg cggcgtgcgt gacacgggcg ttgccttcat ggtcccgacc tgcgcatttc
    14761 tcctgacact gctgcttgtg attgtccttg gcggctttca agcgctgacg catggcggac
    14821 atccgcaacc catcgtggcc cctccgaaac tgcccccgcc cacgactacg cttacgctct
    14881 ggcttttgct caaagtattt tccagcggat gcactgccat gaccggagtg gaagctgtca
    14941 gcaacggcgt gatggccttt cgcgagccac gcacgaaaca cgcaaaactc tccctcacca
    15001 tcatcattgc cctgctgatt gttctgcttg ccggaatagc actgctttgc cgcgcttaca
    15061 atatcggcgc aacagacccg ggcgctcccg gatatgagag cgtgctatcg caactgacgg
    15121 ctgctgttgc cggcaagggc attttttact atgccaccat cggctccatc ctgctcgtgc
    15181 tggcgctttc ggccaatact gcgtttgccg atttccccag gcttgcgcgg gccattgccc
    15241 tgcgcgatta tctgccgcac gtctttattc tgcgtgggcg caggctgctt tattcgcacg
    15301 gggtcatcgc gctggccttc ttcacggctg cattgcttgt gctctttggc ggcgttacgg
    15361 accgcctcat cccgctctat gccatcgggg cttttctggc gtttaccctt tcgcagtctg
    15421 gaatggtagt gcactggcgc aggcagggta atgctaccgg acgcatgatc gtaaatggta
    15481 ttggcgccgt ggccaccgga atcaccgtgc tcgtggtgct ggtggccaaa tttgcagaag
    15541 gggcgtgggt caccgcgctc ctggtgccat tgctgattct ggtcatgaag agtgtgcaca
    15601 ggcattatca gcgggtccag gatgaaacaa cggagccggg agcacttgcc gtgcatgggc
    15661 tgcggccgcc gcgggtcatt ctgcccatgg acaagtggaa ccgcatcagc gaaaaaggac
    15721 tgcgctttgc gctaatgatg tccccggagg tcgaggtcgt ccacgtggat tgtggcgacg
    15781 agcaagacag tatctgtgaa gcctggcacg aaaaagtact caagcccatt caggccgcag
    15841 gactgcccgt accggagctg gtcacgatca aatcgccgta caggtttttt cttcagccgc
    15901 tggtcgagca tgttcttgca gaagaaaaaa agtatggtga ccgccagatt gctgtgcttg
    15961 ttcctgaact ggttgtgcag cattggtggg a
//
LOCUS       DASRBV010000185         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_111744, whole genome shotgun sequence.
ACCESSION   DASRBV010000185 DASRBV010000000
VERSION     DASRBV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111744"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFA02_07095"
     CDS             <1..264
                     /locus_tag="VFA02_07095"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZU09652.1"
                     /translation="THWYNISLGLAMLAGRFLMIVPMMALAGNLAQKKSVPPSPGTFP
                     VNTGLFTVLLAGVVLIIGALTFFPVLSLGPILEHLLLRAGQLF"
     gene            280..2313
                     /gene="kdpB"
                     /locus_tag="VFA02_07100"
     CDS             280..2313
                     /gene="kdpB"
                     /locus_tag="VFA02_07100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZU09653.1"
                     /translation="MAKRQSLWDSEILRQAFVGAFTKLGPRAMVKNPVMFVVEIGSVL
                     TTVLLIQDIVQHRPGFSFDLQITLWLWFTVLFANFAEAMAEGRGKAQADTLRKAKGET
                     MAYRIQKDGRTEAVSSSQLRAGDLIKVEAGQFIAGDGEVIEGVASVDESAITGESAPV
                     IREAGGDRSAVTGGTRVLSDWIIVRITSNPGETFLDRMIALVEGAERQKTPNEIALSI
                     LLSGLTIVFLLAVATLQPFAIYSHAPQTVFVLVSLLVCLIPTTIGGLLSAIGIAGMDR
                     LVQYNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQATEFCPAPGISTERLADAAQL
                     ASLADETPEGRSIVVLAKEKYNLRGRDLTGMHAQFVPFTAQTRMSGVDLPGVSIRKGS
                     ADAIARFLEQQGSSLPRKVNDKVEEIARLGGTPLVVAENDTALGVVYLKDIVKGGLRE
                     RLARLRAMGIRTIMITGDNPLTAAVIAREAGVDDFLAEAKPEDKMALIKREQAKGKLV
                     AMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQLLMT
                     RGALTTFSIANDVAKYFAIIPAMFAGVFPVLNALNIMHLKTPHSAILSAVIFNAIIII
                     ALIPLALHGVKYEPKSAQALLRRNLLIYGVGGMIVPFIGIKLIDVVVNGLHLV"
     gene            2321..2896
                     /gene="kdpC"
                     /locus_tag="VFA02_07105"
     CDS             2321..2896
                     /gene="kdpC"
                     /locus_tag="VFA02_07105"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZU09654.1"
                     /translation="MKQTLIISIRFTLVTTLLLGIAYPLAITAFAHFVFPDKADGQLV
                     MQNGQIIGSRIIGQNFTSDKYFHSRPSAAGNGYDGTSSGGSNLAPSSQKLVSRIEADV
                     AKYHGEANGRPVPIDLVTASGSGLDPDITPAGALYQVHRVAMARGLDESVVRNLVMAH
                     IRPRQFGFLGEPRVNVLELNLALDGLQQQVR"
     gene            complement(2889..4085)
                     /locus_tag="VFA02_07110"
     CDS             complement(2889..4085)
                     /locus_tag="VFA02_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZU09655.1"
                     /translation="MSPVIQEAIERLGPGPLTEESVQQQIAPLFSRVLEGDRIYLANH
                     SLGRPLDVMADDVHEAILLWETKLGDAWDQWLAEREAFRARMAALIHAPRTDCVAPRT
                     SAGQGLRAVLNALPGKPHVLSTRGEFDSIDVILKQYAALGRITVSWVDANAEGNFSVQ
                     EIIRHLRKGMDLVVISQVMFQTGQVVSGLDQLADACHAAGAKLLVDAYHAVGVFPVDA
                     SGMRADFMIGGSYKYLRGGPGAAFLYLSPGILESGLRPLDTGWFAREEVFNFGRTEVP
                     ALKQGGDAFLEATPPVLTWYQARSGQQFTLAMGVERLREYSLKQLTQLKKYLADAGVC
                     AAGGDSQHGAFLTVRHAHAGELVKKLEERRIITDARGEWLRICPDCLTRDEELRAVAD
                     ALCEII"
     gene            complement(4082..4942)
                     /locus_tag="VFA02_07115"
     CDS             complement(4082..4942)
                     /locus_tag="VFA02_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZU09656.1"
                     /translation="MSTAEQNRRPLESGIVTDFRERMSYAGYLCLDGLLSQQRPLSNP
                     PHHDEMLFIIQHQVSELWIKQLIHELTAAIRFVQQDQLDPCFKILSRAKLIQMQLFEQ
                     WAVLETLTPSEYMEFRGVLGNASGFQSFQYRKLEFLLGNKNGDAIKVFAHTPEIYHDL
                     EKTLHAPSLYDEFLRHLKRRGLAVPDACVERDWSAPYQKNEQLVEVFRLIYERPREYW
                     DAYEMCEKLVDVEEYFQLWRFRHMKTVERIIGFRPGTGGSSGVGFLKQALELTFFPEL
                     FAVRTWLKQK"
BASE COUNT         1147 a         1469 c         1408 g         1147 t
ORIGIN      
        1 acacattggt acaacatcag tttgggcctg gccatgctgg caggccgctt cctgatgatc
       61 gtgccgatga tggccctggc cggaaacctg gcgcagaaga aaagcgtgcc gccttcgccg
      121 gggacttttc cggtcaatac aggactcttc actgtgttgt tggccggagt ggttttgatc
      181 ataggcgcgc tgacattctt tccggtttta agcctcggcc ctattcttga acaccttctg
      241 ctgcgcgcag ggcagctctt ttaggcacgg agaagaaaca tggcgaaacg gcaaagtctc
      301 tgggattcgg aaattcttcg acaggcattc gttggcgcat tcaccaagct tggtccgcgt
      361 gcgatggtga aaaacccagt catgttcgtg gtggaaatcg gcagcgtgct cacaacggtt
      421 ctgctgattc aggacatcgt gcagcaccgc cctggcttca gtttcgattt gcagattact
      481 ctctggctct ggttcaccgt gctctttgcg aacttcgcag aagccatggc tgaaggccgc
      541 ggcaaagcgc aggcagacac gctgcgcaag gcgaaaggcg aaaccatggc ttatcgcatc
      601 caaaaagacg gcagaaccga ggctgtttcc agctcacagc ttcgtgccgg agacctcatc
      661 aaagttgagg ccgggcagtt tatcgcaggc gatggcgagg tgatcgaagg cgtggcttct
      721 gtggatgagt cggccatcac gggcgaatcg gcccctgtca tccgcgaagc cggaggcgac
      781 cgctccgctg tgactggtgg aacacgcgtg ctctctgact ggatcattgt ccgcatcaca
      841 tcgaatcctg gagaaacgtt tctcgaccgg atgattgcgc tggtggaagg cgccgagcgc
      901 cagaagacac cgaatgagat tgcgctcagt attcttcttt ccggactgac gattgttttt
      961 ctgctggccg ttgcaacgct tcaacctttt gcgatttact ctcatgcgcc gcagacggtg
     1021 tttgtgctcg tctcgctgct cgtgtgcctt atccctacaa cgattggcgg actgctctct
     1081 gcaattggca ttgcgggaat ggaccggctt gtccaataca acgtgcttgc catgtccggg
     1141 cgagcagtgg aagcagcagg tgatgtgaac actctgcttc ttgataaaac cggcaccatc
     1201 acgctgggca accgccaggc gacggagttc tgtcctgcac cgggcatcag caccgagcgg
     1261 ctggccgatg ccgcgcaact ggcctcactc gcggatgaaa ctcccgaagg ccgctccatc
     1321 gtcgtgctcg caaaagaaaa atacaatctg cgcggacgcg acctgaccgg aatgcacgcg
     1381 cagttcgttc cattcaccgc gcagacgcgc atgtccggcg tggacctgcc cggtgtcagc
     1441 atccgcaaag gctccgccga cgcaattgcg cgtttcctcg aacagcaagg ctcgtcactt
     1501 ccacgcaaag tcaacgacaa ggtagaagaa attgcgcgcc tcggcggaac gcctctggtg
     1561 gttgcggaaa atgatactgc gcttggagtt gtgtatctca aggacatcgt gaaaggcggc
     1621 ctacgcgagc gtctggcacg actgcgtgcg atgggcatcc gcaccatcat gattacaggc
     1681 gacaatcctt taactgctgc cgttattgcg cgcgaagcag gtgtggatga ctttcttgcc
     1741 gaagcaaagc ccgaagacaa gatggcactg atcaagcgtg agcaggccaa aggcaagctc
     1801 gtcgctatga ccggggacgg cacgaatgac gcgcctgctc tcgcacaggc agacgtaggc
     1861 gtagccatga acaccggcac acaagcagcg aaagaagccg gaaatatggt ggacctggat
     1921 tcgaacccaa ccaagctcat tgagatcgtt gagattggca aacagctttt gatgacacgc
     1981 ggcgcgctga ctacgttttc catcgcgaat gatgtagcga agtacttcgc catcattccg
     2041 gccatgttcg caggcgtttt tccggtgctg aatgcgttga acattatgca tttgaaaacg
     2101 ccacactcgg ccattctctc ggctgtcatt ttcaatgcaa tcatcatcat tgcgctgatt
     2161 ccgcttgcgc tgcacggcgt gaagtatgaa ccgaagtctg cgcaggcatt gctgcggcgc
     2221 aacttgctta tctatggagt gggcggaatg attgttcctt ttattggcat caagctgatt
     2281 gatgttgtgg tgaacggctt acatctggtc tgaggaagat atgaaacaga cgcttatcat
     2341 ctcgattcgc tttacgctgg tcactacgct tctgctgggt attgcctatc cgcttgcgat
     2401 taccgcattt gcgcacttcg tgtttccaga caaagcagat ggccagcttg tgatgcaaaa
     2461 cggccagatt atcggatcgc gcatcattgg gcagaacttc accagcgata aatattttca
     2521 cagccgccca tctgcggctg gcaacggtta tgacgggact tcatccggag gctcgaacct
     2581 tgctccttca agccagaagc tcgtttcacg cattgaggct gatgttgcga agtaccacgg
     2641 ggaggccaac ggacggcccg tgccaattga cctcgtcact gcttctggtt cagggcttga
     2701 cccggacatc acacccgccg gtgcgctcta tcaggtgcat cgtgtagcga tggcacgtgg
     2761 gctggacgag agtgttgtgc gcaatctggt gatggcgcac attcggcccc ggcaatttgg
     2821 cttcctcggc gaaccgcgcg tgaatgttct tgagctgaat ctggcgctgg atggattgca
     2881 acagcaagtc agataatttc acacaaggcg tccgcaaccg cgcgtaattc ctcatcccgc
     2941 gtcaggcagt caggacaaat ccgcagccat tctccgcgag catctgtaat gattctgcgc
     3001 tcctccagct tttttactag ctcacctgcg tgggcatggc gcacggtcag aaaggcccca
     3061 tgctgagaat cgccgccagc agcacaaacg ccggcatccg caaggtactt ctttaattgc
     3121 gtaagctgct tcaggctgta ctcgcgcagg cgctctacgc ccatagccag cgtgaattgc
     3181 tggccgctgc gcgcctgata ccacgtaagc accgggggag tggcttcgag aaatgcgtct
     3241 ccgccctgct ttaaggcggg aacttccgtg cgcccgaaat tgaagacctc ctcgcgggca
     3301 aaccatcctg tatccagcgg acgcagaccg ctctctaaaa taccgggcga aagatacaga
     3361 aatgccgctc ccggcccacc cctcaggtat ttgtaactgc cgccgatcat aaagtccgcg
     3421 cgcatgcccg aagcatctac aggaaatacc ccaaccgcat gatacgcatc taccagcagc
     3481 tttgcgcctg ctgcgtggca cgcatcagca agctggtcga gtccgctgac cacctgtccg
     3541 gtctgaaaca tcacctgcga aatgacaacc agatccatgc ccttgcgcag atgacggatg
     3601 atctcctgta cagaaaaatt gccctctgca ttcgcatcaa cccaactcac tgtgatgcgc
     3661 ccgagcgctg cgtactgctt cagaatgacg tcaatcgaat cgaattcgcc gcgagtgctt
     3721 aaaacatgag gcttgccggg aagcgcattc agaactgcgc gaagcccctg tccggccgaa
     3781 gtcctgggcg caacgcagtc cgtgcgcgga gcatgaatga gcgcggccat gcgtgcccgg
     3841 aatgcctctc gttcggcgag ccactggtcc catgcatcac ccagttttgt ttcccacagc
     3901 aggatggcct catgcacatc atccgccatc acatccagcg gtcttcccag ggagtggttc
     3961 gccaggtaaa ttctgtcgcc ctccaacacg cgcgaaaaga gcggagcaat ctgctgctgc
     4021 acggattctt ccgtaagcgg gccggggcct aggcgctcga ttgcttcctg aatcactggg
     4081 ctcatttctg tttcaaccat gtgcgcaccg cgaataattc aggaaaaaat gtaagctcca
     4141 gcgcctgctt cagaaatcca acgccggagg agccgcctgt tccgggacga aagccaataa
     4201 tgcgttcgac tgtcttcatg tggcggaagc gccagagctg gaaatactct tctacatcca
     4261 ccagtttttc gcacatctca taagcatccc agtattcgcg cggacgctca taaattagcc
     4321 ggaagacctc caccaactgc tcattcttct ggtaaggtgc cgaccagtcg cgctccacgc
     4381 acgcatccgg cacagccagt ccacggcgct tcaggtggcg cagaaattca tcgtaaagac
     4441 tcggcgcgtg tagcgtcttt tcgaggtcgt gataaatctc cggcgtgtgg gcgaagactt
     4501 tgatcgcgtc gccgttcttg ttgcccagca ggaactccag cttgcggtac tggaacgact
     4561 ggaacccgga ggcatttccc aggacgccgc gaaactccat gtactccgac ggagtaagcg
     4621 tttcaagcac cgcccattgt tcgaagagct gcatctggat gagtttggcg cgcgaaagta
     4681 ttttgaagca cggatcgagc tggtcctgct gcacaaaacg aatcgctgcg gtaagctcgt
     4741 ggatcagttg tttgatccac agttccgaca cctgatgctg gatgatgaac agcatctcgt
     4801 catggtgcgg cggattcgac agcggacgct gctgagaaag caggccatcc agacacagat
     4861 agcctgcata gctcatgcgc tcacggaagt ccgtaacaat gccggattcc aggggtctgc
     4921 ggttctgttc ggctgtagac ataaaaattt ggcctgacag aacattgtct gtcaggccag
     4981 ggaggcatac aaccatagga aaactaacaa aacgcaacag aatgctcgtc gcagataaga
     5041 tttgtcaata cttctgttac aaatttttgt ttttgtctcg ccatatgaga tagaacgcac
     5101 aaacgctcgt aaagcggttt gcctgatgtt atcttgcgct catgcgaaaa cttctctgga
     5161 ttctcgctct t
//
LOCUS       DASRBV010000186         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_365287, whole genome shotgun sequence.
ACCESSION   DASRBV010000186 DASRBV010000000
VERSION     DASRBV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365287"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFA02_07120"
     CDS             complement(<1..548)
                     /locus_tag="VFA02_07120"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU09657.1"
                     /translation="MSLKEIKNIGSAALLCCCLGMGGRALAQNTGSIVGTVTDPSGGV
                     VPRAHVVAQSLEQGLERSVSSSEAGSYVLPQLPIGTYSVTVEADGYQKFVSENVKVDA
                     DQNVRVDALLQPGHVSQQITVSAAPPLVDTHSDTISAIIPHELVNDMPTSTGQPLDFI
                     SVLPGVSDVSTRAAFAPDRNGPT"
     gene            718..3690
                     /locus_tag="VFA02_07125"
     CDS             718..3690
                     /locus_tag="VFA02_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZU09658.1"
                     /translation="MPVVRNAVVLALLFLGVRAAMASAANPITTNFATTSYRVRDGLP
                     EDIVQAFAQTSDGYLWIGTTGGLVRFDGARFAVLSNQRGPAFHEISVHCLAASKDGSL
                     WIGTEGSGLIQYRNGTFHAYTSNEGLSDMFVRAVFEDSSGHLSIGTNDGFFQLVGDKI
                     KRLDGVGGLPVLAINAIQEDDRHRLWIGGSRLMVGDQGRFEERTLPGYHSRNRVKSIL
                     QTSDGAMWIGTVSGLYRSPDGTAPFQRIPGIQGTVRVLRQSKDGLLWMSVVGQQGAVY
                     RVSSTTPFLAPVVPLPGTILSVFQDTEQNIWVGTQTGMVRYSRTPVSILPLPDARASD
                     FGTLYLDADHTLWYAANHLVHVRDDIASQYHFPTLAGTKIRNLMRDRAGTLWLGTDGN
                     GLYHLTGDHIEHFTTEQGLVNNFIRAIIQAHDGSLWIGTDEGVSRLRDNRFTNYGTSD
                     GLAYFSIRSMLEDHNGDIWIGTDQGLSHFSRGQFVSDAAVNNLREEKVWAIHEDKDGG
                     LWFGTRYHGLYRYKRGSLFHYGMTDGLASNGVYQILEDASGHFWMSGPGGISLLDRHE
                     LDAYAEHHRRALSLTFYTLPDDDGPVQLFGGVQPAGCITPQGDVWFPGNLGPVHVSLA
                     QSQQQTMPRLKIDQVLADGRPISQTSEIALAPGNSSLEISYSPVMLRDQRNVRFRYKL
                     EGLEKRWNDALLRRDISYINLPPGHYIFRVEGFETNAPNDVSSVSLMIVKRPYFYRTW
                     WFTLLMAVTMAAVILAVYRARVARMRQRFLAVLEERNRIAREIHDTVIQGCTSVSAVL
                     EAVSTISPAQAGLREDLLGRARAQVRATIEEARRAVWDLRQKAAPGGSLNTAMMGMAA
                     QIFSEFGIPVYCNLQRRPFPASKAAMHEILMIVREALHNAALHGSPSAIHLLSILEQD
                     TLTIEIADDGQGFSLKSLTGNGHKHYGVTGMRERAVRLGGSLEIASSAEAGTRVILRV
                     PKSTAAIEENIAEQAGIL"
     gene            3687..>4264
                     /locus_tag="VFA02_07130"
     CDS             3687..>4264
                     /locus_tag="VFA02_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU09659.1"
                     /translation="MSQNKTKILVVDDHPLMRVGIAAIIDAQPDMMTIAQAGSAEEAV
                     RQYAQHHPDIILVDLRLPDKSGVEIIRCIRSKNANAKFIVLTTYDGDEDIHQALEAGA
                     RGYIIKGMPYETLIDALRRVHAGNRYLPRPIEKILESRMPDSDLSPREREVLALIVAG
                     KSNKEIATALGISRATVKCHVSEIFSRMGVDDR"
BASE COUNT          946 a         1174 c         1193 g          951 t
ORIGIN      
        1 gtagggccgt tgcggtccgg agcaaatgcc gccctggtgc tgacatcgga aacgccgggg
       61 agcacggaaa tgaaatcgag aggctgtccg gtactggtag gcatatcatt gaccagctca
      121 tgaggaatga ttgcgctgat ggtgtcagaa tgtgtgtcta ccagcggcgg tgctgcgctg
      181 accgtaatct gctgcgacac atgccctggc tgcagaagcg catctacacg gacattctgg
      241 tctgcatcta ctttgacgtt ttcactgaca aacttttgat agccatccgc ctcgacggtc
      301 acagaatatg tcccgatagg aagctgcggt agaacatacg atccggcttc gctggatgag
      361 acggagcgtt ccaatccctg ctcgagagac tgtgccacta catgcgctcg tggaacaacg
      421 ccgccgcttg ggtccgtaac tgtccctaca atgctacctg tattctgcgc gagcgcgcgt
      481 ccgcccatcc ccaggcagca acagagcagc gcggcacttc caatgttttt tatctctttt
      541 aaactcacaa catggccctc ctgaacaaat gaagcagcgg acatccggtt tttgcttcat
      601 ggcacaaaat catagcggtg ctgcgagtgc ctctcatcgg ccaaatggcc gatgcctggc
      661 ggaaaaatcc ggacaaccat gtttctggcc ttgtatactc gtctttccag agtcgaaatg
      721 cctgttgtcc gcaacgcggt tgttcttgcg ctgctctttc ttggcgtccg tgccgcgatg
      781 gcaagcgctg ccaatccaat tacgacgaat ttcgccacta ccagttatcg cgtgcgcgat
      841 ggcctcccgg aagacatcgt gcaggcattc gcccagacct ccgatggtta tttatggatc
      901 ggcactacag gcggattggt ccgctttgat ggcgcccgct ttgctgtcct aagcaatcaa
      961 cgcggacctg cctttcacga aatcagcgta cattgccttg ctgcctcgaa agacgggtct
     1021 ttgtggatag gcaccgaggg cagcggcttg attcaatatc gcaatggaac atttcacgca
     1081 tatacctcga atgaaggttt gagcgacatg tttgtgcgcg cagttttcga ggacagctcc
     1141 ggccatctct cgatcgggac aaacgacggc tttttccagc ttgtcggcga caagattaag
     1201 cgcctcgatg gagttggtgg actgcctgtg ctggccatca atgcgattca ggaagacgat
     1261 cggcaccggc tttggattgg cggttcccgg ctcatggttg gagaccaggg gagattcgag
     1321 gagcgtactt tgccgggata tcacagccgt aatcgcgtga aatctattct gcagaccagt
     1381 gacggcgcca tgtggatcgg caccgtttcc gggctttacc gctcaccgga tggtactgct
     1441 ccatttcagc ggataccggg aatccagggt acggtccgcg tgcttcgtca gagcaaagat
     1501 gggttgctgt ggatgagcgt tgttgggcaa cagggtgcag tttaccgcgt ctccagtact
     1561 acgccatttc ttgctcctgt ggtgcctttg cccgggacca ttctgagcgt ctttcaggac
     1621 acagagcaga acatatgggt gggcacgcaa acaggcatgg tgcgctacag ccgcacgcct
     1681 gtcagtattc tcccgttgcc ggatgcccgc gcatcggatt tcggtacgct gtatctcgat
     1741 gcagaccata cgctttggta tgcagcaaac cacctggtcc atgttcgcga tgatattgcc
     1801 agtcaatatc atttcccaac cctggccggg accaaaatac gcaacctcat gcgtgaccgc
     1861 gccggaacac tgtggctcgg caccgatggc aatggcctat atcatttgac gggcgaccac
     1921 atagagcact tcacaacaga gcaaggtctt gtaaacaatt tcatccgcgc catcattcag
     1981 gcccatgatg gcagcctgtg gatcggaaca gacgaaggcg taagccggct gcgcgacaac
     2041 agatttacaa attatgggac cagcgacggt cttgcatatt tcagcattcg gtccatgctt
     2101 gaagaccaca atggcgacat ctggataggg accgaccagg ggcttagcca tttcagccgt
     2161 ggtcaatttg tctctgatgc tgccgtgaac aatttgcggg aagaaaaggt atgggccatc
     2221 catgaagaca aagacggagg actctggttc ggcacacgtt atcatggcct ctaccgctac
     2281 aaaagaggaa gtctgtttca ttatgggatg accgatggac tggccagcaa tggtgtctat
     2341 cagattctgg aggacgcctc tgggcacttc tggatgagcg gtcccggagg tatctccctt
     2401 cttgaccggc acgaattgga tgcttacgcg gagcaccatc ggcgcgcgct ctccctcact
     2461 ttctacacgt tgccggatga cgacggcccc gtgcagcttt ttggcggagt gcagccagca
     2521 ggttgcatta cacctcaagg cgatgtttgg tttcctggca atctgggccc ggtccatgta
     2581 tcgctggcgc agagtcagca acagacaatg ccccgcttaa aaattgatca ggtcctggcg
     2641 gatggacggc cgatatcaca aacatcagaa attgcgctgg cgccgggaaa cagcagcctt
     2701 gaaatttctt actcgccggt catgctgcgc gaccagcgta acgtccgttt tcgctacaaa
     2761 ctggagggcc tggagaagag atggaacgat gctttgcttc gccgcgatat ttcgtatatc
     2821 aatctgccac caggccacta tatctttcgc gtggagggct ttgaaacgaa tgcccccaat
     2881 gatgtttcgt ccgtttcact gatgattgtg aagcggccgt acttctacag aacatggtgg
     2941 tttacgcttc tgatggcagt gacaatggcg gctgtcattc ttgctgtgta tcgcgcccgg
     3001 gtggcccgca tgcgtcagcg gttccttgcc gtgcttgaag aacgtaaccg aatcgcgcgt
     3061 gaaatccatg acacagtcat tcagggctgc accagtgttt ctgccgtgct ggaagctgtc
     3121 tccacgatct ctccggcgca ggccggcctg cgggaagacc tcctcgggcg ggcgcgggcc
     3181 caggtgcgcg ccacaattga agaagcgcgg cgggccgtat gggacttgcg gcaaaaggca
     3241 gcgcctggtg gaagtctcaa cacagccatg atgggaatgg ctgcgcaaat cttttctgaa
     3301 tttggaatcc cggtgtattg caatttacag cgccggccgt ttcccgcaag taaagctgcg
     3361 atgcatgaaa ttctgatgat cgtgcgcgag gcgttgcaca atgctgctct ccatggatca
     3421 ccttcagcca ttcatctgct ctcaatcctt gaacaggaca ctctgacgat tgaaattgca
     3481 gacgatgggc agggcttcag cctgaaatcc cttacaggta acggacataa gcattacggg
     3541 gtgacgggga tgcgcgaacg ggcagtgcgg ctggggggca gcctggagat cgccagttcc
     3601 gcggaagcgg gcacccgtgt catcctgcgc gtgccaaaaa gtaccgcggc cattgaggaa
     3661 aatattgcag agcaggcagg tattttgtga gccagaacaa gaccaaaatt ctcgtcgtag
     3721 acgatcatcc tttaatgcgg gtcggcattg ctgcaatcat tgacgcgcag ccagacatga
     3781 tgacgattgc ccaggccggc tccgcggaag aagcagtccg gcagtatgcc caacaccacc
     3841 ccgacatcat tctggtagac ctgagactgc cggacaaaag cggtgtagaa atcatccgct
     3901 gcattcgcag taaaaacgcc aacgcgaaat tcatcgtgct tactacatat gacggcgacg
     3961 aggacatcca tcaggcactc gaagcaggcg ctcgaggata catcatcaag ggcatgccct
     4021 atgagaccct cattgatgcg ctgcgccgcg tccacgcggg caaccgctat ctgccgcgcc
     4081 ccattgaaaa gatcctcgaa tcgcgcatgc ctgattctga cctcagcccg cgcgagcgcg
     4141 aagtgctggc cttaatcgtc gccggcaaga gcaacaaaga gatcgccacg gcactgggca
     4201 tcagccgcgc tacggtcaaa tgccatgtaa gcgaaatctt ctcgcgcatg ggcgtagatg
     4261 atcg
//
LOCUS       DASRBV010000187         7210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_725294, whole genome shotgun sequence.
ACCESSION   DASRBV010000187 DASRBV010000000
VERSION     DASRBV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725294"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFA02_07135"
     CDS             complement(<1..908)
                     /locus_tag="VFA02_07135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZU09660.1"
                     /translation="MSALQVWDRAWQMVESRQAAADMLFVYAVRTTGVYCRPSCPSRR
                     PSRSAVEFFATSELAERAGYRACKRCAPNREHPQTHMLTQACDYIERNLDSTISLEKL
                     GKITGLSAFHTQRLFRRYLGISPRQYQQARRMERFRQNLLTHDSVTTAMYEAGFASSS
                     RLYESAHEHLGMTPTAYRKGGKDVTIRYAITDSPLGKMLVAATDVGICATAFGSLASE
                     LEDDLAARFPQARRERDHTGLGEAVKKILALMTEHPVALELPLDVRATAFQRRVWEAL
                     RRIPRGQTRTYSQIAKEIGQPTAVRAV"
     gene            complement(1301..2473)
                     /gene="metK"
                     /locus_tag="VFA02_07140"
     CDS             complement(1301..2473)
                     /gene="metK"
                     /locus_tag="VFA02_07140"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZU09661.1"
                     /translation="MSARDKFLFTSESVTEGHPDKIADQISDAILDACLEQDPYSRVA
                     CETLTATGLVVIAGEITTQAYVDFQTLVRGVVAAIGYDNALYGFDSNTCAVISSINKQ
                     SGDIAMGVDTGGAGDQGMMFGYATNETEELMPAPISWAHKLTRQLSAVRKNGRLPYLR
                     PDGKSQVTVEYGSDGRPARIDAVVISTQHAETVSNEELRADILKNVIQAVLPANLLDE
                     NTKYHINPTGRFVIGGPMGDSGLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSAAY
                     MARHIAKNIVAAGLADRCEVQLAYAIGVPEPVSVLVDTFGTGRIEARKLEELVRANFK
                     LTPKGIIESLDLRRPIYRKTAAYGHFGRNDKDFTWEATDKAAALREQAGLKEAVAK"
     gene            complement(2693..4600)
                     /locus_tag="VFA02_07145"
     CDS             complement(2693..4600)
                     /locus_tag="VFA02_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HZU09662.1"
                     /translation="MNGKTFAIAAALASALLAPAAYSQNSPLLTVHVDKTAAKVSPTL
                     YGLMTEEINYSYDGGLYAEMVRNRTFRSDWSGIRYWYLVEDGTSQAKIDIDKQSGPSK
                     VLDASLRIDVQKADEKSKAGVLNEGYWGFALHPNTTYEGSFYAKADSGALGPVTVSLV
                     NNQSNKVVASAAVSGVTTDWKQYHFTLKTGAIQPSSANHLEITVTHPGTLWLNLVSLF
                     PPTYHNRPNGNRIDLMEKLAAMHPQFLRFPGGNYLEGNHISERFEWKKTIGPLVDRPT
                     HPSPWGYHSSDGMGLLEFLEWCEDLHMQPVLAVYAGYSLEGEHVNPGADLEPYVQDAL
                     DEIEYVTGDANTKWGAERVKDGHPQPFKLTYVEIGNEDWFDRSGSYDARYAQFYKAIK
                     AKYPGLQLIATAPVKGVRPDVIDEHYYLRATQFFHDASHYDKTDRNGPKIFVGEWATR
                     EGTPTPNFGAALGDAAWMTGMERNSDIVIMASYAPLLVNVNGMQWETDLIGYDALRSY
                     GSPSYYAQVMFASHLGDERPEAGLEGVDTANPKLFYSVTKDTQKKRLYVKLVNASSIP
                     QNVEVRLDGANIAKTGKLTTLSAAGTQATNTIDEPKNIVPVESTLKDVSSDFHHTLPA
                     YSIQVLELDLQ"
     gene            4806..5342
                     /locus_tag="VFA02_07150"
     CDS             4806..5342
                     /locus_tag="VFA02_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZU09663.1"
                     /translation="MYKSVISLLNRKSSSRRRRQAGFTLMELLIVISIMLILMLIAIP
                     NMLNLRSQANETSAIQSLRAIYQAQIQYQTNYPANGFACSLQALGGDANAGPPSAQNA
                     QLLQHDLATGSKAGYTFNITNCTKTTVNNQDMYTSYEATAVPQAVGKTGHRGFCIDMT
                     GEIKADPAGGTNCTVPIQ"
     gene            5415..6116
                     /locus_tag="VFA02_07155"
     CDS             5415..6116
                     /locus_tag="VFA02_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZU09664.1"
                     /translation="MRNRSESALRKRVSQFLRARPRVFVGFAVLLWAAGAPAQTLTPE
                     ALALRIDRHYNALHSLSVHFTQKYDGMGMHRTESGILLLKKPGKMRWTYSSPAGKLFV
                     LDGHDAWFYSPGDSEIPRVPAKKLDDLRSPLRFLLGHTQLEKELTNLQVAPAENGAWT
                     LTGVPKGMEKRIASVAITASADGTIQSMRIEETDGAINTFVLSGEVPNAPASDSDFIF
                     QAPAGTHIVEGTPPV"
     gene            complement(6185..>7210)
                     /locus_tag="VFA02_07160"
     CDS             complement(6185..>7210)
                     /locus_tag="VFA02_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU09665.1"
                     /translation="VIMILSIVGIMIGQVSNARNQTGGIGADMIVRPPNASFVTGVGG
                     APVPAKVADVIARLPHVAVAAPVITDFNMAGSVETLWGIDYKSFNALRPFVFLSGGPF
                     QGPNDVIVDDVFARADHGHHVGDTIKIKDHPFRICGIVEHGKGGRKFLPIHTLGALMG
                     NPDNASLFYIKSDDLKNQDLIRQEILATPGLGQYQVQTIQEWMSLMTPEHLPGFTPAL
                     NSVIGIAVIVGFIVVFQTMYTAVMERTREIGILKSLGASRFYITNVVLREAALVSIAG
                     IVLGIALTYLVKLGLSYKFPTIPFPVTIQWRINAALIAFTGSLLGALYPALKAARKDP
                     IDALAYE"
BASE COUNT         1403 a         1986 c         2262 g         1559 t
ORIGIN      
        1 actgcgcgaa cagccgtggg ctggccgatt tccttcgcaa tctgcgagta ggtgcgtgtc
       61 tggccgcgag gaatgcggcg cagcgcctcc cacacacggc gctggaaggc cgtcgcgcgg
      121 acgtcgagcg gcagctcgag agcgaccgga tgctcggtca tcagggcgag gattttcttc
      181 acggcctcac cgaggccggt gtggtcgcgt tctcttctgg cctgtggaaa gcgcgcggca
      241 agatcatctt ccagctcgct ggccagcgaa ccgaatgccg tggcgcagat gcccacatcg
      301 gttgcggcca cgagcatttt tcccaagggg gaatcagtaa tggcatagcg gatggtcacg
      361 tctttgccgc ctttccggta cgcggtgggg gtcatgccca ggtgttcatg ggctgattcg
      421 tacaggcggc tgctcgatgc gaatccggcc tcatacatgg ctgtagtgac actgtcgtgc
      481 gtgagcaggt tctggcggaa acgctccatg cggcgcgcct gctgatattg gcggggcgag
      541 atgccgagat agcgacgaaa gagccgctgg gtgtggaagg cgctcaggcc tgtgattttg
      601 ccgagttttt cgaggctgat ggtgctatcg agattgcgct caatgtaatc gcacgcctgc
      661 gtcagcatgt gggtctgagg gtgctcccgg ttgggggcgc agcgcttgca ggcgcggtat
      721 ccggcgcgtt cggccagctc gctggtagcg aaaaattcga ccgcactccg cgatggcctg
      781 cgacttgggc acgaaggccg gcaatagacg cccgtggtgc gtacggcata tacaaagagc
      841 atgtcggcag cggcctgcct cgattccacc atctgccatg cccggtccca aacctgaagt
      901 gcgctcatat cagcctcgct ttcgtttttc aggtttgatc ttcgcgcagg tgcaggggat
      961 gcggcactcc gcttcttgcg ctcgaatttt tgtttttcgg gcatgtcctt aacagcttag
     1021 agcgaagaac gttaaagttc ccgaagacac acctacgggg agtaaatgac tctgcgtacc
     1081 tgtgcgtctc ctttactgaa ggaaaaaggc agatccgaag acctgcctta ggcagctgac
     1141 aaagtcaggc cttaagagaa aacacccctc agcggctgaa gccgcattcc tattgcgagg
     1201 tttatggcac ggctaaaagc cgtgcccctt caaagcattc gctttgtcat ccatctgagg
     1261 caggcccgaa gacctgcctt tcctgactag ctggcccgct ctactttgcg actgcttctt
     1321 tcagcccggc ctgctcgcgc agggcagcgg ccttgtctgt ggcttcccag gtgaagtcct
     1381 tgtcgttgcg gccgaagtgg ccataggctg cggtctttct gtagatagga cggcgcaggt
     1441 ccaggctctc gataatgccc tttggcgtca gcttgaagtt ggcgcggacc agctcttcga
     1501 gctttctggc ttcaatcctg ccggtgccga aggtgtctac gaggacgctg accggctcgg
     1561 gcactccaat ggcgtaggca agctggactt cgcagcggtc ggcgagtccg gcggccacaa
     1621 tatttttggc gatatggcgg gccatgtaag cggccgagcg gtccaccttt gtcgggtcct
     1681 tgccgctgaa ggcgccgccg ccgtgacggc ccattccgcc gtaggtgtcc acgatgatct
     1741 tgcgcccggt caggccggag tcgcccattg gaccgccaat cacaaagcgg cctgtcgggt
     1801 tgatgtgata tttcgtgttc tcgtcgagaa ggtttgccgg gaggacggcc tgaatgacgt
     1861 tcttcaggat gtcggcacgc agttcctcat tggagacggt ttcggcgtgc tgcgtcgaga
     1921 tgaccacggc atcaatgcgg gcgggccggc catcggagcc gtactccact gtaacctggc
     1981 ttttgccatc gggccgcagg taaggcagcc ggccgttctt gcggactgcg ctcaattgac
     2041 gggtgagttt gtgcgcccag gagatggggg ccggcatcag ctcttcggtc tcgttggtgg
     2101 cgtaaccgaa catcatgccc tggtcgccgg cgccgcctgt gtccacgccc atggcgatat
     2161 cgcccgactg cttgttgatg gaagaaatca cggcgcaggt attggagtcg aagccataga
     2221 gggcgttgtc ataaccgatg gcggcaacca cgccgcgcac gagcgtctgg aagtccacat
     2281 aggcctgggt ggtgatttcg ccagcaatca ccacgaggcc ggttgcggtg agggtctcgc
     2341 aggccacacg gctgtagggg tcttgttcga ggcaggcatc gagaatcgcg tcggaaatct
     2401 ggtctgcgat tttatctgga tggccttcgg ttacggattc agaggtgaat aaaaacttgt
     2461 cgcgtgcgga caatcgagtt ctcccttatg ttgcaatggg cctgccggga ggcataaagc
     2521 acctttacga tggtacaaga ccagaatacg cagcgcaaag catagcagcc aaatgggagc
     2581 cgggggctgt caggcgggga ataagggggg cgagcggggt ttcttccgaa atccttctgc
     2641 tccgcccttt tcggcccgga aaacaggcag aaaagggcga gggcctcagg ggctactgca
     2701 aatccagctc taatacctgg atggagtatg caggcagagt gtgatggaag tcgctgctga
     2761 cgtccttgag cgtgctttcg acagggacaa tgttctttgg ctcatctatc gtattggtgg
     2821 cctgggtccc tgcggcgctc agtgtggtca gcttgccggt ctttgctata tttgcgccgt
     2881 caagcctgac ttccacgttt tgcggaattg aagaagcatt taccagcttc acatacaggc
     2941 gcttcttctg tgtgtctttt gtgacggagt agaacagctt ggggttcgcc gtatccacgc
     3001 cttccagacc ggcctcgggg cgctcatcgc ccagatggct tgcgaacatt acctgcgcgt
     3061 aatagctcgg tgagccgtag ctgcgcagcg cgtcgtagcc gatgaggtcc gtctcccact
     3121 gcatgccatt tacgttgaca agcagcgggg catacgaggc catgatgacg atgtcgctgt
     3181 tgcgctccat gcccgtcatc catgcggcat cgcccagcgc cgcgccaaag ttgggcgtcg
     3241 gggtgccttc gcgtgtggcc cattcaccga cgaagatctt agggccgtta cggtctgtct
     3301 tgtcgtagtg gctggcatcg tggaagaact gcgtggcacg cagatagtag tgttcgtcaa
     3361 tcacatcggg gcggacaccc tttaccgggg ccgtcgcaat cagttgcagg ccggggtatt
     3421 tcgccttgat ggccttgtaa aactgggcgt aacgcgcatc atagctgccg gagcggtcaa
     3481 accagtcctc attgccgatt tctacataag tgagcttgaa gggctgggga tggccatctt
     3541 ttacgcgctc agcaccccac tttgtattgg cgtctccggt gacgtattcg atttcatcca
     3601 gcgcgtcctg cacgtaaggc tcgaggtcgg cgccgggatt gacgtgctcg ccttcgagcg
     3661 agtacccggc atagacggcc agcaccggct gcatgtgcag gtcttcacac cattcaagaa
     3721 attcgagcag tcccattccg tctgaggagt ggtagcccca cggactggga tgggtggggc
     3781 ggtctaccag cgggccgatg gtcttcttcc actcgaagcg ctcggagatg tgattgcctt
     3841 cgagataatt tccgccgggg aagcgcagga actgtggatg catggcggcc agcttctcca
     3901 tcaggtcaat gcgattgccg ttgggacggt tgtggtaggt gggagggaaa agcgaaacga
     3961 gattgagcca gagcgttccc ggatgggtaa cggtgatttc cagatgattc gcagacgagg
     4021 gctggatggc gccagttttc agagtgaagt gatattgctt ccagtccgtg gtgaccccgg
     4081 aaacagcagc gctggccaca accttgttgc tctggttgtt taccaggctt acggtcacag
     4141 gcccgagcgc tccggagtct gctttggcat agaacgagcc ttcatacgta gtgtttggat
     4201 gaagggcgaa tccccagtag ccttcattca acactccggc cttgctcttt tcatcggctt
     4261 tctggacatc aatccggagg ctggcatcga gcaccttgct ggggccggac tgcttatcaa
     4321 tgtcaatctt cgcctgggat gttccatcct cgaccaggta ccagtaacgg atgccggacc
     4381 aatcgctgcg gaatgtgcgg ttgcggacca tctccgcgta caggccgcca tcgtaggagt
     4441 agttgatctc ctcggtcatc aggccgtaga gcgtagggct gactttggcg gccgtcttgt
     4501 ccacgtggac ggtgaggagc ggagaattct ggctgtaggc cgcgggcgcc aggagggcgg
     4561 aggcgagcgc cgcggcgata gcaaacgttt tcccattcat tgcgagatct tccttgaggg
     4621 caaaaaatag atgccgcgcc agtatatcgc acagaacacc aaaacgctga atactatgtg
     4681 ggtccttgct ttttctgcag tgggcctgag gcagagcgag gcgaccgtgg tgcgtgagcg
     4741 ctctactggc gctgtgcgtc ttattcattt agcatttctc tcgacaggcg gaagtgccac
     4801 gacccatgta caaatctgtt atttctctgc tgaatcgcaa gtcttcctcg cgccgcaggc
     4861 ggcaagccgg gttcacactg atggagctgc tgattgtcat ctcgatcatg ctgatcctta
     4921 tgctcatcgc cattccgaac atgctgaatt tgcgcagcca ggcgaatgag acctcggcca
     4981 tccagtcact gcgggccatc tatcaggcgc agattcagta tcagaccaac tatcctgcga
     5041 atggctttgc ctgctcgctg caggccctgg gtggggatgc caacgccggt ccaccgagcg
     5101 cccagaatgc gcagttgctt cagcacgacc tggccaccgg ctccaaggcc ggatacacgt
     5161 ttaatatcac caactgcacc aagacgacgg tgaacaacca ggacatgtac acgagctatg
     5221 aagccaccgc tgttccgcag gccgtgggta agaccggcca ccgcggcttc tgcattgata
     5281 tgacgggcga gatcaaggcc gatccggcgg gcggtaccaa ctgcacagtg ccgatccagt
     5341 agtgaacgat tttcaactac accacaccag tgaacgggct tacatgggta cccaggccct
     5401 ggagcataag cagagtgaga aacaggagtg agtcagcgct tcgcaagcga gtcagccaat
     5461 tcctgcgtgc gcggcccagg gtctttgtgg ggtttgccgt tttgctttgg gccgcaggcg
     5521 cgcctgctca gacgcttacg ccggaggccc tggccctgcg cattgacagg cattacaacg
     5581 ccctgcactc gctctctgtc catttcacgc agaaatatga cgggatgggg atgcaccgca
     5641 ccgagagcgg catcctgctg ctgaagaaac ccggcaagat gcgctggacg tactccagtc
     5701 ctgcggggaa gctctttgtg ctggatgggc atgatgcctg gttctattcg ccgggagact
     5761 ccgagatccc gcgcgtcccg gctaaaaagc tggacgatct gcgctcgccg ctgcgctttc
     5821 tgctggggca tacgcagctt gagaaagagc tgaccaatct tcaggtggcg ccggctgaga
     5881 acggggcgtg gacactcacg ggtgtcccga aaggcatgga aaagcggatt gcctcggttg
     5941 cgattaccgc cagcgcggat gggacgatcc agtctatgcg gatagaagag acggacggcg
     6001 ctatcaacac ctttgtgctg agtggcgaag tgccgaatgc ccctgcgtca gacagcgatt
     6061 ttatctttca ggctccggcg ggtacgcata ttgtggaggg cacgcctccg gtatagtttg
     6121 aaactccgaa aaagcaggtc ctacttcggc aaactcaggg caggccccac gacacagccc
     6181 gggattattc gtaagccaga gcatcaattg ggtctttgcg cgcggccttg agcgcggggt
     6241 agagcgcccc cagcagagag ccggtgaatg cgatgagcgc cgcgttgatg cgccattgaa
     6301 tggtcaccgg aaaggggatg gtcggaaact tatagctgag tcccagcttt acgagatacg
     6361 tcagcgcgat tccgagaacg attccggcga tggagaccag ggccgcctcg cgcagcacga
     6421 cgttcgtaat ataaaagcgc gacgctccca gcgatttcag gatgccaatt tcccgggtgc
     6481 gctccatcac ggcggtgtac atggtctgaa agaccacgat aaagccgaca atcacagcga
     6541 ttccgatgac gctgttcagg gcgggtgtga atcctgggag gtgctcgggg gtcatcagcg
     6601 acatccattc ctggatggtc tgtacctgat actggccgag gccaggagtt gccagaatct
     6661 cctgccggat gaggtcctgg tttttcaggt catcgctttt gatgtagaag agcgaggcgt
     6721 tgtcgggatt gcccatgagc gcgccgaggg tgtggatggg aaggaacttg cggcctcctt
     6781 tgccgtgctc gacgatgccg cagatgcgga aggggtggtc cttgattttg atggtgtcgc
     6841 cgacgtgatg tccatgatcg gcgcgggcaa agacgtcatc tacgatgacg tcgtttggac
     6901 cctgaaacgg gccaccggag agaaagacaa aggggcgcag cgcgttgaaa cttttgtaat
     6961 caatgcccca gagggtctcc accgagccgg ccatattgaa gtcggtaatg acgggtgcgg
     7021 ccacggcaac atgaggcagc ctggcgatga catcggcgac tttggccggt acgggcgcgc
     7081 ctccaacgcc ggtgacaaag ctggcgttgg gcgggcgcac gatcatgtca gcgccgatgc
     7141 ctccggtctg gtttctggca ttggagacct gcccgatcat gatgccgacg atggagagga
     7201 tcatgatgac
//
LOCUS       DASRBV010000188        43750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_570510, whole genome shotgun sequence.
ACCESSION   DASRBV010000188 DASRBV010000000
VERSION     DASRBV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 43750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43750
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570510"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFA02_07165"
     CDS             complement(<1..536)
                     /locus_tag="VFA02_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZU09666.1"
                     /translation="MENPLPSVRAQMEAHPEIPLAEALPPDPSLPPLAQIRRFDTHRL
                     IPAKYSANLESVLTRVADNDGHLQHIFELDNATNARLEAEENLRPGITQRELVFNVPH
                     YRIINAAFTHPNPLGARFSTSDRGAWYAGFELATSKAEIIFHKSVEFAEINWREREEL
                     QYDEYLADFTGSFHDLRPA"
     gene            complement(641..1843)
                     /locus_tag="VFA02_07170"
     CDS             complement(641..1843)
                     /locus_tag="VFA02_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU09667.1"
                     /translation="MKSPMRISLRKALIFCALAGFLQAETPVPAQQQDQQQTQQNQQN
                     QQQQQDQNAPEAGGPAGDTGAIAVPKKKEKEEEAPPPPPEPKVKNPPGLENYSLRVNV
                     PVVNVDVGVVLEKTHQFVPNLQKENFRVYEDGVPQQITDFRRIQAPITAVLLVEFAAN
                     SWWFIQDMENAAYAFAQQLKPDDYIAVVMYDMHTQILTDFTQDKRLVYEALNSLTIPT
                     WQETNLFDALYETLDRLTRVDGRKYIILISSGRDTFSKITLDKVLQKIKATPNVTIFS
                     VGTGQTARILADSYGMMGPIRNLDYLQADNQMTTFARMTGGMAFFPRFEGEMPDIFHQ
                     INESIRNEYVLSYHPTNQKLDGTWRKIRVELVDNEGHPLRMQDEKHHPLKYDIIARDG
                     YKAQQQVE"
     gene            1945..3735
                     /locus_tag="VFA02_07175"
     CDS             1945..3735
                     /locus_tag="VFA02_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55"
                     /protein_id="HZU09668.1"
                     /translation="MITRLKDIGWLILCTATALGGQTVSASSPLPAAPPPYTAGFYPL
                     DQVHRGLHGVAYTVFEGTKPEPMGVEILGVLHNALGPGQDMILARLEGTKPEYTGVVA
                     GMSGSPVYVDGRLLGALSYRIGMFSKEPIAGITPISQMLEVAHDHPYLMASNGSGTAV
                     KPASSDVIQTDAIQPMDTPLVFTGFSQQALEFWKEHAPPALGATAVSGIGGSSSDEKQ
                     PDPIVPGSAVSALLMRGDLEIAATCTVTYVDPKQLLACGHPITQFGPVSMPMTKAQVV
                     ATLPSAYNAFKIINTTETVGSFTEDRESAILGHFGQQAKMIPVTVNITGEPAPHTLHM
                     EAVDQPQITPSVVMVAVFQGLMERNDFTAETSYRVRSTVDIPGYPAVHFTGLAAPGEL
                     PANLTAALMVGEQFSRLYDNAARQAPIHRVNIEVEAIPKRLTAQLESAQSADTIVHAG
                     QTVVIHAAIRPWRGEIRNIRIPVTLPAVLPQGQVRLLVSDGSTLDRLTQSPSRFMNSQ
                     PLDVAATIEQLNSQHENDHLYVTLLDPSPQAVLDGRTLTTVPVSLANVLEPMRQNQKL
                     TLNGESAVPLASVPIDSVLNGQQVLTLKVE"
     gene            complement(3746..4855)
                     /locus_tag="VFA02_07180"
     CDS             complement(3746..4855)
                     /locus_tag="VFA02_07180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZU09669.1"
                     /translation="MQFVDLKAQYRRLKQGIDARIQAVLDHGQFILGPEVVELEERLA
                     ARTGSKYCITCASGTDALLLALMALGIGAGDEVVTSPFTFFATGEMIALLGATPVFVD
                     IDPDTYNIAPDKIEAAITPRTKAIMPISLYGQCADFDAINAVAEKHGIAVIEDAAQSF
                     GATYKGRQSCNLSTIGCTSFFPSKPLGCYGDGGACFTSDDELARRMFELRNHGQDRRY
                     HHVSIGINGRLDTIQAAVLLAKLDVFDDELALRRKSAAMYDRLLGDAVRVPVIRDGNT
                     SAYAQYTIEVDERDALESALKQAGIPTAVHYPVPLHLQPVFAGMNHGHGSFPNAERAG
                     HRVLSLPMHPYLEEEQIVRIAGEVRRIASPVSSSR"
     gene            complement(4924..5754)
                     /locus_tag="VFA02_07185"
     CDS             complement(4924..5754)
                     /locus_tag="VFA02_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09670.1"
                     /translation="MKRLTEFFAGLCLSACGLTALAQQPTEGVMQPPKVLVVHREFIK
                     PGKAGAVHVKSESAFIQAMTDAKWPTHYFAVTSLSGPSRALFIMGYDSFDAWEKDNLA
                     MMKDAKLSAAFDNAALADGELLSGYDDNVAVYREDLSYHAGVNIARMRYFEISRYVVR
                     PGHRKEWEALVKEYMNGYQKGVPDVHWAVFESVYGQDNGGVYLVFIPLKSLSELDHFS
                     AESKNFEAAVGEAGMKRIEDLTASCIESSQTNLFAFDPRMSYPAETWVKADPDFWSTK
                     "
     gene            6001..6354
                     /locus_tag="VFA02_07190"
     CDS             6001..6354
                     /locus_tag="VFA02_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU09671.1"
                     /translation="MSKSKLDLLQGTLDLMVLQTLAAMGALHGYGIARRIEQISGNEV
                     LLNQGTIYASLVRLQQRGWIAASWGTSENNRKAKFYSITKTGRKQLAQDAAYWERLSG
                     VMGRVLAMQSEGGEQ"
     gene            6351..9002
                     /locus_tag="VFA02_07195"
     CDS             6351..9002
                     /locus_tag="VFA02_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09672.1"
                     /translation="MIYGLPEALRRLRSFFRKGPVDQELDAEMASHLEMSMEENLKRG
                     MSPDEARRQALIQFGGVQQAREHHREARGLPWLDVLIQDLRFAFRTLRRDRGFTIVAV
                     LILALGIGANIAVFSVVNTILLRPLPFRDPQRLVRIVEKGAGTNESGRTYTADATQDF
                     QQQNHTFQSVSGYFAFSSPDNFKLTGNGQPIPVTGILVAEGFFQTLGVDPALGRLFRP
                     EEFAKHAQPVALLSYPFWKRQFHSNRSIIGHTVNFNNTSVTIIGVLPDSFDFGSVFSP
                     GAKVDLFVPYIMDDFREDGNDLALIGRLKPGVSLSAAQRDADEIFPKLLFEHKHPEFK
                     PEYTGQLTVLKDYVSGKLQRSLIVLWCAVGMILLIACVNLSNLLLARAAARSKEFAMR
                     SALGAGRGRLVRQWLTESLVLSGAGALLGLGLAYTVISYLAHQGSLALPLLSSVRIDG
                     TVLTWTLLTTLIAALLFGLAPGLRVSRVNLQEELKDGGHGTSEGRKHDHMRSALVVTE
                     VALACVLLVGAGLLLRSFLHVLDVDLGFEPGHAAAISVDYDSGGNPAKQAAIWQDVVG
                     RVSVLPGVEAAGISDNLPMSRNRGWGISAKGEKQQSDSPFVGTFVYIISPGYLKAMGM
                     HLMEGRDISWEDLVNNQNVVIINESVARRLWPGQDPIGRTARTGGTDVRVIGVIADVR
                     ESGAEDNAGNQEYLPATSQFGPEGANLVVRSKLPPSALATSVMRTLRQINPGQPATEF
                     KPIQTLVDHATSPRRFFVLLVGTFAGLGLLLASLGIYGVISYSVTRQTQEIGIRIALG
                     ATQGHVLRTVIWRTLRLALIGIAAGTIASFAVARMIASLLFGTVPTDPITFTGVVVLL
                     TLVALVAGLIPARRAARIDPMIALRTN"
     gene            complement(9149..10051)
                     /gene="flgL"
                     /locus_tag="VFA02_07200"
     CDS             complement(9149..10051)
                     /gene="flgL"
                     /locus_tag="VFA02_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZU09673.1"
                     /translation="MRFDPLYLNNAITSLDQASALQQQLTNELSSGKRVNSLADDPVA
                     AGENVLLASQLQMNDSFSQTETSVVGRLQVADSTLGSVVSQLTQAVSLAMQGNNGTLN
                     ASDLQSIASQLSGIRDEVVSLANTTYMGTYLFSGSKGNTAPFSLNTSVTPNTVTYNGD
                     SVVSYVMTPNGQQIQTNVPGNAIFNNPSNDVLGVLNNLVAQFSSGAPSAASVADATAL
                     NSALNYVSQQRVVLDNSITRLQAAQNYTQMQGTQITAAQTDLLQTDVAKVSTQMSTAE
                     TQQTALIQVVNLLEKNNGNLFSLL"
     gene            complement(10073..11479)
                     /gene="flgK"
                     /locus_tag="VFA02_07205"
     CDS             complement(10073..11479)
                     /gene="flgK"
                     /locus_tag="VFA02_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZU09674.1"
                     /translation="MATLNSAFSIATGALEAAQSAINVIANNVSSASTPGYTTELPQW
                     QENVTVNIGGVDYGQGATMTGPSSQRDLVLEQRIQQQTQLQQDTNARYTALDQVQSIF
                     SSATSTSSSSLDIGQSITGFFSALSQLQSNPSDPSLRQSVLTAATTLVNAFHAASSQL
                     SSQQNSVDHQADTIVSQVNSLTQTIAGLNQKIAEMSPNGDAGQMEDQRQYDIEQLSQL
                     VGIHQIQTEDNGLTITTANGAPLVVKDQAFSLNTALSGGVTHISDAHGNDITSGLAGA
                     GGQLGGILTVRDQDIPQMQSALDTLAYDFGTALNVANQAGTDANGNPGGLIFNLPAAS
                     AGAASQISVAISSGSQIAAAASGNGPSDTSNLVSMLQLQNQAIAGGTTASSYYSGFVS
                     SLGSLVSQVSTENTALQASLTQLTTQRDSLSAVNLNDQAALLENLQQTYQAASKVFTI
                     LDTVMASALNLGVETSVS"
     gene            complement(11467..11730)
                     /locus_tag="VFA02_07210"
     CDS             complement(11467..11730)
                     /locus_tag="VFA02_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09675.1"
                     /translation="MSRVGLSQTPIVALFERAIEALKRADAASIGEVLSDCDSVKAAG
                     SQEEFSRALAQRAVFSKLLEQTSENLRILRGETGTARTRGWQR"
     gene            complement(11744..12037)
                     /gene="flgM"
                     /locus_tag="VFA02_07215"
     CDS             complement(11744..12037)
                     /gene="flgM"
                     /locus_tag="VFA02_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZU09676.1"
                     /translation="MNINDGLHNLTEATGVTETSRISPVQRQTSVSSPGTGATAQDSA
                     DVSAAAALANKAMQAADVRMDKVAAVQQAIHNGTYNVSPEDVAEKLVRQMIGK"
     gene            complement(12082..12405)
                     /locus_tag="VFA02_07220"
     CDS             complement(12082..12405)
                     /locus_tag="VFA02_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09677.1"
                     /translation="MNSVSFSSSTAVSGEIKPNPRLESAAHEFEASLMKEFLKPLEED
                     GLFTDGDSATGSADTLMSYGAEAMARGISQHGGFGIATQILRHFHSTAQVDENSHRIG
                     NGQTL"
     gene            complement(12402..13517)
                     /locus_tag="VFA02_07225"
     CDS             complement(12402..13517)
                     /locus_tag="VFA02_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZU09678.1"
                     /translation="MRCARLGYLLLLLWPLTAWPYERVKLARVKDVATVEGVRDNQLV
                     GYGIVVGLRGTGDSQQTVFPLQTLVSTLQRMGVNMQQSPQLSQMRTQNMAAVFISATL
                     PAFARPGNKIDVTVSSAGDARSLEGGLLLLTPLYGPDGQIYAEAQGPLVLGGYAVGAN
                     GNLKQYNHPTTGRVPDGAMVERAVPLDLSRMKTLSLLLNDADFRTAEMMAAAINKEMA
                     RPLAHAVDSRRITLAVQSSDDVPSLLAQVESVEVETYPRAKVVVNERTGTVVIGGDVR
                     LQPVSILHGGLSVNVVTEYQVSQPAPFSQGTTQVVPQTTIQTQDQPVNRIELKQGATV
                     DELVRNLQQIGASARDIISILQAMKEAGALEAELEVL"
     gene            complement(13529..14245)
                     /locus_tag="VFA02_07230"
     CDS             complement(13529..14245)
                     /locus_tag="VFA02_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZU09679.1"
                     /translation="MMRKQTRTGAVLCFVLCGVAFAATHKPEKQSKPTPPDIALNAYI
                     ERVRAENAAEEHSPGAIWTPDGRLTRLSADVKACHVHDPIAIVVSESLAASTDGTVKN
                     SRASNASSQVSSLFGKLSAGNALNNLVNQASASGLNAQGESVTNSSLSTTLGGEVVDV
                     LPNGMLVVQAVRQLTFNQQTQLIKMRGLVRPEDVDAQNRVLSTAITSLEVEVVGKGII
                     NDYTYRPNAAVRFLQKLLIF"
     gene            complement(14242..14673)
                     /locus_tag="VFA02_07235"
     CDS             complement(14242..14673)
                     /locus_tag="VFA02_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HZU09680.1"
                     /translation="MRHLGWVLLLLVSPAFGAECSARGPGSVVVRYIFDGVLHRYWAV
                     VADCAHPERPWVTEEISQANAEIEAKTALQATPVVEAGAKVILRKNEDGARIVLTATA
                     LEPGRTGDSIRVRIGSGAVLEGKIRGLGQVELIGKAKWNGQ"
     gene            complement(14680..15468)
                     /gene="flgG"
                     /locus_tag="VFA02_07240"
     CDS             complement(14680..15468)
                     /gene="flgG"
                     /locus_tag="VFA02_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZU09681.1"
                     /translation="MIRALYTAASGMSAQQLNLDTIANNLANSSTSGFRQRRLQFQDM
                     IYQNLVTPGAAQSQQTVSAGLQVGLGTKSAATEVIMTQGDLNQTGNPLDLAIQGNGFF
                     QIQRPDGTIAYTRAGSFHLNNQGQIVTIDGDPLIPSITIPPNATSIAITQYGVVNATL
                     PGQQNPTQLGQIQLATFANPEGLNSIGNNLFEATLSSGQAVLDTPGGTQGLGTLQQGY
                     LENSNVDVVTEFVQMVLAQRAYESNSKVIRAADDMYSQVNNMTR"
     gene            complement(15487..16218)
                     /locus_tag="VFA02_07245"
     CDS             complement(15487..16218)
                     /locus_tag="VFA02_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HZU09682.1"
                     /translation="MDSGYYAAFSGWLARSEALDAAASNLANANTSGFRAQREYFRNA
                     IVGPEALNSQVNQIVNNYGVLGGNHLYLGQGALTHTGNPLDLAIEGQGFFAIQTKQGV
                     RYTRDGSFRRALNGTLVTASGEPVLNAQNKPIQVPTGDIVVGVDGVLSVGGAVAGKLG
                     VFDVPASALVPEGANRYAVTAAAKPTVTAAAIHQGALETSNEDVIQGTLDLVLVQRQA
                     EMMQKALSSFYVNFDKTASEDLPKV"
     gene            complement(16290..16778)
                     /locus_tag="VFA02_07250"
     CDS             complement(16290..16778)
                     /locus_tag="VFA02_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZU09683.1"
                     /translation="MMATPARELLQESAHAQDLDSAVEEVFRLMMGANCAPAEEYVFG
                     NNETITAVIGLAGAMSGACTLRCGMDAALRMAECMVGVRPAELDDMVKDAIGEVCNMV
                     AGAWKGKHPVLASGCMLSTPAVVTGTNYQLHMQQPEFRIDRSYRFEEHAVNISIVCES
                     IQ"
     gene            complement(16811..17194)
                     /locus_tag="VFA02_07255"
     CDS             complement(16811..17194)
                     /locus_tag="VFA02_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU09684.1"
                     /translation="MSDINALIVDDSSVMRKIVERALRQAGLSLNKVYEASSGAEALD
                     VLKSEQVQLILSDINMPMMDGLEFLRQMRAQDLAPDVPVVMITTEGSEEHVKAAIAAG
                     AQGYIRKPFTPEQVKDRVLPLLELV"
     gene            complement(17217..18131)
                     /locus_tag="VFA02_07260"
     CDS             complement(17217..18131)
                     /locus_tag="VFA02_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09685.1"
                     /translation="MKRAERGERPGSIASPFLPQCGNPNCKSGWLRLWRSREVPVFEG
                     KWVCSTVCAREMIEAAVLREARDGNLSPAMHQHRVPLGLLLFSKGHITQEQLKRALEA
                     QKKAGSGRLGEWLVRQKAVEQEQLVRALGAQWGCPVLSADIFHAAAMAPALPRLLADS
                     FGALPLRVAGRSLLYVAFESRPDPCIALALERMTGLKCETGILRDSEFERAHGALLRA
                     PFPRVRLLETTSLRGAAHAMTAMVEESRPAEARLVRVHNYFWLRLWKKSPSAGQKHSL
                     PAAGDIEDMVCSFSTEFRWCSSGEESCR"
     gene            complement(18131..18862)
                     /locus_tag="VFA02_07265"
     CDS             complement(18131..18862)
                     /locus_tag="VFA02_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZU09686.1"
                     /translation="MKAFLDAWLTEVPMGFSQELAGMVKLAQAAAMPEPEKALCFQLE
                     VGGGLHGVFWVLVSAEKLAELLMAARVTADGMDPRRDAQLWEGMLRQVAERTAKAVGK
                     TSGATVSIASVREAPFPAGEKFAFYELRFGEVIVPIGFADRTEKPKMKEAFNPRGVDL
                     LLDIELDASLRFGSREMPLGEVLELGPGDVVQLERHVTDPVDLIVGDKIVARGEVVLV
                     NGNFGLRVTEVAEPRKSLESIRCLF"
     gene            complement(18880..19869)
                     /locus_tag="VFA02_07270"
     CDS             complement(18880..19869)
                     /locus_tag="VFA02_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZU09687.1"
                     /translation="MEGEGTRKVERSLKQDEIDALFQAARARSKAASQETRSRVQPYS
                     FSRAGQISNEQLRAISLLNDIFARNLTHNLGVWLRSRFRVNLVSAEQMPFNEFMMRIP
                     DLAYVSSVRLEPLRALSVLQLDLALAPPMIDLLLGGEGRPDKVRELTDIEESILTSVA
                     EVICRELSTAWQPVGLSFSFERRQLHTQIARIMPVSEKTLCLSFEIQMPGASGMLNLA
                     FPAVVSNTVLRRLTGDWSRQHRHSAETRQRMQERVRLVKVGASLQLPPVRLEGRQLEP
                     LREGQVLKLPLMASHPAELRIAGIPLFEAHPVQVEGQRAGHLARTTPAAARRI"
     gene            complement(19869..20759)
                     /locus_tag="VFA02_07275"
     CDS             complement(19869..20759)
                     /locus_tag="VFA02_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HZU09688.1"
                     /translation="MDELEMRNLGAMANRPFTEEEMRRKAAMLSLAGTEEEKQKKSAP
                     KKTPVRDGNAARFTPEQERMMLEHLPVVRYIARRIHERLPQHVEMEELVSAGVLGLMD
                     AFQKFDPAKKVQFRSYAQFRIRGAILDSLRTLDWSPRELRRKGRAVEQAIRVLTARLG
                     RTPSENEIAAELGMELGDYHQLLGELKGLEIGTLHMERSEDSGEEELAYVPNRQEDDP
                     LFRCLEGELRERLAEAIRKLPERERLVMTLYYYEEMTMKEIGLTLGVVESRVSQIHAS
                     AVLRLRAMLADLAAKRAQEK"
     gene            complement(20768..22882)
                     /gene="flhA"
                     /locus_tag="VFA02_07280"
     CDS             complement(20768..22882)
                     /gene="flhA"
                     /locus_tag="VFA02_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZU09689.1"
                     /translation="MSASVVVPAAAKPATGQGVLARIAGFGKDLWLPVAAISILFVML
                     VPVPAAVLDLLLACSFSASVLVFLSAVQLRRAVDFSVFPSLLLLLTLFRLSLNIASSR
                     RILLHGHEGTAAAGSVIEAFGQFVVGGNYLVGFVLFLALVAIQFLVVSHGAVRTAEVT
                     ARFTLDALPGKQMAIDADLNAGLIDEAAARKRRQAIAQEAEFYGAMDGAARFNQRDAL
                     ATILITAINIVAGLLIGVLQQGAAISEAARTYTVLTVGDGLVTLIPSLLVSVAGGMVL
                     TRANTASGLTTELGAQLLRKGATLYIASGVLALLGLVPGLPKLAFLLPAATLGYIGWR
                     VSKKQQSEAAAGPEQKQKAAVQTENIASLLKMDELSLEIGFQLIPFVDEKQGGQMLAR
                     VRALRRQLASELGFVVPAIHISDNLRLRPREYVFSLRGIEIGRWQIENNALLAVETGR
                     IAQPVPGKETVEPAFHSSARWISPALENQAIAAGYSVVDGVTAITTHLAEMVRQHAHE
                     LLGRAETKRLLDALNESHPRLVEELVPKVLALGEVQKVLQQLLREQVPIRDLGTILEV
                     LLDAAQVNRNLPQLVEAVRQAMGRRIVQPLLEEDGTLRILQLDPALDEEMAAAFSGET
                     GALLLSDGRPRGVVWFKRLVDSVKQLIAQQPNLAIPVLLCSSPARFYLRRWMEPVLPK
                     VVVLSPLEIPPDVRVRSVGTVR"
     gene            complement(22879..23976)
                     /locus_tag="VFA02_07285"
     CDS             complement(22879..23976)
                     /locus_tag="VFA02_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZU09690.1"
                     /translation="MSGERTEKATPRRRQKAQEQGDRVYSREWTAACAMLSGVLALGM
                     AAPHWTSLWSGTMTQMLAVASPSAWNDAGFMQQALVLRHVIFVSLSPLVLLCGTAATG
                     ALLAGMAQGGGVRFHAQHLQPKLSRIHPATSIRQMFSLQGSSRLLKSLLPVAVLLFFA
                     FRQIEGQLALPPMSVEHLPRMFSAAYTLLLDSAWVLFAWSALDYIVQWRAREGRLRMS
                     KQELREEMKESEGSPQVRSRIRGLQRQMRRRKLKADVKKATVVITNPTHYAVALSFDF
                     ETMEAPKVLAKGRNLLAEQIKAEARWAGVPIVENPPLARSLYRSVEEGQSIPVDLYAA
                     VAAILAWLYRKQVEERIRRERTAAQKAKEKA"
     gene            complement(23973..24767)
                     /locus_tag="VFA02_07290"
     CDS             complement(23973..24767)
                     /locus_tag="VFA02_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZU09691.1"
                     /translation="MEELFARGNTDWAHYLSALVLVLVRLGGMVVAAPFFSSQALPKQ
                     VKAIFVIATGVLLAPAVAALPMAHAGLGISAVLGELSIGLVFGFSLTLMDEILLFAGQ
                     VLGVQFSFSLANLLDPNSQVETPLMSQMFSLMGVLVLIAAGLDRTILAALMRSYVAAP
                     LGGAFLNGEGALVLVRMMGGVFFAALQLAAPVLAATMLVEIAVALIGKMSPQLPVMAV
                     TVPAKTITGYVLLLGSLALWPRFIEARFTKLLDVAEGLLRQGVTHG"
     gene            complement(24770..25078)
                     /locus_tag="VFA02_07295"
     CDS             complement(24770..25078)
                     /locus_tag="VFA02_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HZU09692.1"
                     /translation="MEPAGRFSAEELLMGPDQIAELMRYLLKEALIVSAPVLVLASVT
                     SFLISLFQTLTSLQDQTLSTVPRIVIVAVALLIGLPWFLRRLVYFTVVLLGDLHRYIG
                     "
     gene            complement(25039..25740)
                     /gene="fliP"
                     /locus_tag="VFA02_07300"
     CDS             complement(25039..25740)
                     /gene="fliP"
                     /locus_tag="VFA02_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680648.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZU09693.1"
                     /translation="MNAMLLLFAKAPKLLSLPGSQQHLINNNGVPWTIVFALTLITLL
                     PAILLSMTPMVRLLVVFHFLRQALGTQTAPSNQVLMGLGLMMTWFLMQPVLSAVDRQA
                     VEPFRQGQISGMEAIQRGIGPPKQFMLKYAREKDLALFTSAAATERPKTPADLPMRVV
                     VPAYMLSELKAGFVIGAVLFLPFLLVDMVVASITTSIGMFQLPPVVVSTPLKILLFVM
                     VDGWNLLAGSLLRSF"
     gene            complement(25737..25997)
                     /locus_tag="VFA02_07305"
     CDS             complement(25737..25997)
                     /locus_tag="VFA02_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZU09694.1"
                     /translation="MTAMTQAAVPGRLGGVVSAFLHSLKKQVGSVNGVLRIEERLSLG
                     PKKMLFLVDCNGHKFLVATGADTIVSMTEIRPEEKRRKAEKA"
     gene            complement(26011..26337)
                     /locus_tag="VFA02_07310"
     CDS             complement(26011..26337)
                     /locus_tag="VFA02_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZU09695.1"
                     /translation="MTTDISTSAEGQTTVAADSSQVPVVLEKPVDETSLAALASKLPL
                     QLDLRVPVPSFRVQSLLSLEKGQIVETIWPHTEDLPLWAGDVQLVWTEFEVVDQKLGV
                     RVTRLA"
     gene            complement(26411..27010)
                     /gene="pyrE"
                     /locus_tag="VFA02_07315"
     CDS             complement(26411..27010)
                     /gene="pyrE"
                     /locus_tag="VFA02_07315"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZU09696.1"
                     /translation="MTNYRDALLKLLARISFKLGEFRLSSGGTSDYYIDCRLTTLHAE
                     GGRLTGLALLELLREHKLQPAAVGGLTLGADPIVSNLATASAWYAQEHHGSPFIHGFL
                     VRKTEKAHGTGRRIEGFLEQDAPVFIVDDVCTTGASTITAIEAAREAGMKVIGVACLV
                     EREEAGGRAAVEAAAKEAPFFSLFTANDVRAAHVRQLAL"
     gene            27055..27546
                     /locus_tag="VFA02_07320"
     CDS             27055..27546
                     /locus_tag="VFA02_07320"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU09697.1"
                     /translation="MTFLEALRIEFDDEVNKTRKILDRVPEKSLAWKPHEKSMTLGRL
                     SGHVAELPSRPAAISQDIFTRQANYVPFAATSHVELMQRFEESVSEGRMALQQFTEAD
                     MAKPWTLKLGDKVILEMPKWTALRAVVMNHMIHHRAQLGVFLRLLDVPVPGMFGPSAD
                     EKL"
     gene            complement(27596..30529)
                     /gene="treY"
                     /locus_tag="VFA02_07325"
     CDS             complement(27596..30529)
                     /gene="treY"
                     /locus_tag="VFA02_07325"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZU09698.1"
                     /translation="MPKSTYRVQVHSGFTFADVEKILPYLHDLGISDLYSSPIFEARS
                     GSTHGYDVTRHDRINPVLGGEEGFHRLAAALREREMGLLLDIVPNHMGVGNESVWWQD
                     VLENGRTSEFASFFDIEWEPIKPDMRGKLLLPVLGNQYGEELESGKIQVAIQDGHLKI
                     KYYDHSFPMAPRSIPVLFPEREDEREGVPASFRDLLKELAHIPPNDSSDPGLIAQRQQ
                     QLRDLKPRLIKALQSPEIQPIVERAIQRINGVEGDPRSFDQLHALLELQPYRLAYWRV
                     SSEEINYRRFFDINDLVGLRQEDPACFAATHRLVRRMIASGEVTGLRIDHCDGMFNPR
                     QYLIRLQLLCIAGQCFGPEPKGELTANGIEVVLRETLRGYDWTRSAGPLYTVVEKILE
                     PREALPQEWPVRGTTGYDFLHLANQIFIEPRNEAKFTSLYAKILGREVRPDELIYRCK
                     LQVMQTSLSSEIYVLTNLLNYLAASDRRARDFTESILERVIRETIACFPVYRTYIDDR
                     GQYTERDITFIVQAVERAKHRNPDVAESAFDFLKNTLLLGNKDDEKYSARLHFALKFQ
                     QLTGPVMAKGVEDTAFYVYTRFLSSNEVGSSIRSFGITTELFHATNLDRLQKSPDSML
                     TTSTHDTKRSEDVRNRLNVLSEMPSQWASMVRRWMRMNAVYKQKINDGRTAPDPNEEY
                     LIYQTIVGAWPWRMETADDRQRFLNRIKDYCTKAFSEAKINLSWTNPDPEYLDAVHKF
                     IENILLPQGRNQETRFVHTLKSFMPAVQFFGALNSLSQIVLKIASPGVPDFYQGTELW
                     DLSLVDPDNRRPVNFEERVDALRRLREQAEREGPAAVCTNVLQTLADGRIKLWTMHRA
                     LSLRNELHSLFRHGDYLALNIEAEKQENAVAFLRQDAATKESVLAIVPRFACSLMGMR
                     TNLPLGDAWGKAQVILPGQAPRSYVNVFTGEEIQSGDDGVLRLQDVFTQFPVALLRSK
                     PSISQ"
     gene            30726..30908
                     /locus_tag="VFA02_07330"
     CDS             30726..30908
                     /locus_tag="VFA02_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09699.1"
                     /translation="MPLSGEAIRLMNYIDDVTVTLRRLLSLAQTLPPEERKRVGEYLK
                     NAQPNINTVIQALEGK"
     gene            complement(31023..31298)
                     /locus_tag="VFA02_07335"
     CDS             complement(31023..31298)
                     /locus_tag="VFA02_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09700.1"
                     /translation="MTSVDVLFRYEQHPSEAAMFALSNLSEVYGIRRIQLNENEQTIR
                     VEYDATRLNKAVVGQLLRRAGISISEEISLIPPQPPAEMEAAAVAVK"
     gene            complement(31453..31686)
                     /locus_tag="VFA02_07340"
     CDS             complement(31453..31686)
                     /locus_tag="VFA02_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZU09701.1"
                     /translation="MTCTEFIALLDDLMEDRVSASLRAELEEHLRGCEHCVVTLNTTR
                     KTIEIYRSHELYELPNELRERLQKAILAKCKKC"
     gene            complement(31683..32312)
                     /locus_tag="VFA02_07345"
     CDS             complement(31683..32312)
                     /locus_tag="VFA02_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU09702.1"
                     /translation="MPDIQTQGTEEVHPDVLLVNRAREGDVEAFEKLVKQYDRQVFRI
                     AQHITQNREDAEDVVQDSFLKAYEKLDQFQGNSKFYTWLVRIAVNEALMRLRKRRTGR
                     MVSIDEDVETEEGSMPRDLADWGPDPEQQYSQSELAEILRKTIQGLPPGFRVVFVLRD
                     VEGLSTEETAETLGLSVPAVKSRLLRARLQLRERLSRYFRKNKRNGGAK"
     gene            complement(32451..33371)
                     /gene="hemC"
                     /locus_tag="VFA02_07350"
     CDS             complement(32451..33371)
                     /gene="hemC"
                     /locus_tag="VFA02_07350"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZU09703.1"
                     /translation="MIRIGSRGSQLALWQANHIADALRRAGHEVSIEIIRTTGDAVSN
                     VAFNKVGTKGMFTKEIEEALYGGRIDLAVHSLKDLPTEISPAFAIAAIAKREDPRDAF
                     VSLKYGSFAALPSGARIGTSSLRRQAQLRAQRRDVELVEFRGNVDTRLRKLEDGQVDA
                     IVVATAGLERLGRTEWVRERFSPLVVCPAPGQGALAMETRSDDARIRAAIAFLDDEET
                     HFAVTVERTALGALGGGCHVPIGIYCRNTPEGIVVSGAVAHPDGSSLVRVELRHESGQ
                     AAEQLGHLTAERLLEQGAREILAPASEQTG"
     gene            complement(33368..34702)
                     /gene="hemA"
                     /locus_tag="VFA02_07355"
     CDS             complement(33368..34702)
                     /gene="hemA"
                     /locus_tag="VFA02_07355"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZU09704.1"
                     /translation="MRILLTGVNHKTAPVELREQLAIAPERLGEATLALRHFPGVSEA
                     MVLSTCNRVELLACHESAHPDLLVFLQKFLGVDTTQLRPHVYEYREGEAVRHLFRVAS
                     SLDSMVVGEPQILGQVKESYTVARAVGAVQTHLERLLQSTFTVAKKVRSETQIGSSSV
                     SIASVAVDLAKKIFGSLSGKRILLVGAGKMSELAARHLMQQGAHSLLVANRTFDRALR
                     IAREFEAQAVRFEDLYATADQADIIITSTGSQHHIFRREHGQQFLHKRRNRPMFFIDI
                     AVPRDVDPEMNRVDGVFLYDIDDLQSVAASHLADRAREAQRAEEIIAQEVLRFQRKAQ
                     TLNVTPMIVELQNTAEDIRQAELRRVQARLQSLTPEQQAAVESLTRGLMNKFLHLPMQ
                     SLKAAAQESDALVIEVLREIFGLSSVQEQPSGPALVESQDVPVAIGGRKARP"
     gene            complement(34699..35514)
                     /gene="ccsA"
                     /locus_tag="VFA02_07360"
     CDS             complement(34699..35514)
                     /gene="ccsA"
                     /locus_tag="VFA02_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZU09705.1"
                     /translation="MFLLWLRVAAALYGLASVSALPAVLGDRPRWQKASLVLAIAGWF
                     YHVVAMVEILVAAHRWMPVGMHEVQSTLSLLIVAAFLLIWLRYRAISFSVFALPLAFF
                     LLLMPSFGPDRYTFSSPLVRSGWIYIHIALLLAAYAALIFSLLASLLYLVQERRLKNK
                     RTPGFLNWLPPLDTMDQIAQKTLVLGFYCMTGGLLIGSFIAQEHVGPTYFLDPKVVLS
                     FVMWVLYIVMVFVQRSTGLRGRRAAYLSSAVFLVMLCVWAANLFSSVHRYTTP"
     gene            35629..38268
                     /gene="mutS"
                     /locus_tag="VFA02_07365"
     CDS             35629..38268
                     /gene="mutS"
                     /locus_tag="VFA02_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZU09706.1"
                     /translation="MRYSGLSVPETSVQSIETAQTPLMRQYAAAKREHPDALLFFRLG
                     DFYELFFEDAYIASRELQITLTARDREKSQPMCGVPYHAAEGYIARLLRKGYRVAICE
                     QMEDPKLAKKIVRREVTRVLTPGTAIDPALGSEQNNFLAAVHVEDKLAALALLDLSTG
                     DFRTTEFTGPQALAQCLDELEKAAPSEVLLAASASFDNTSALDRIRTKTSIEDWVFTS
                     DYAVPLLERQLGTASLDGFGLAHHPAAAIAAGAVLHYVRQTQRTDISHIDSIRFYERA
                     EHLQLDQVTVRNLELVEPIFSEVGRETTLFQTLDACQTPMGKRLLRASILRPLLNEHS
                     IEQRYEAVDLLVRDLVLREELRRCLGGILDLERLLARISLDSAGPRDVLALAASLQHL
                     PPLRQILEKPEAPLWKQITERLDPLEDVCGTIRQTIRPDPPLTLADGDVIAQGVDAEL
                     DELRALSQSGRQSIAAIEERERTRTGISSLKVRFNSVFGYYIEITKANLANVPPDYER
                     KQTLANAERFTTPELKDYETRVLTAHERSIEIEKRIFAGLRRSVLEHASRIRKSSSAV
                     AEADMLANFAHLAALRNYSRPQLVSESVLEILAGRHPVVERLMEVSGEGSFVPNGLYL
                     DVEGPAILLLTGPNMGGKSTYLRQAALLTIMAQMGCYVPAERMRYGLVDRIYTRIGAS
                     DNVSRGRSTFMVEMTETATILNTATLRSLILLDEMGRGTATFDGLSLAWATLEYLHAK
                     IGARTLFATHYHELTILAEQLPRLKNLRVAVKETPQGIVFLHSIEPGPANKSYGIEVA
                     RLAGLPPQVIQRARQVLKQHERSERHNVAAETEAPLQLTMFTPLSQRIIDRLTETDIN
                     ALTPLQALNLLEQLQNEIKGGKA"
     gene            38265..39386
                     /gene="nagZ"
                     /locus_tag="VFA02_07370"
     CDS             38265..39386
                     /gene="nagZ"
                     /locus_tag="VFA02_07370"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZU09707.1"
                     /translation="MSETLRQQVGKLLIVGLEGTALTAVERAWLRLIRPGGIILFRRN
                     IEEAAQTAALLRETTEIIGAPIFRCVDLEGGLVDRLRDLIAPMPSAAQVFATGKPALY
                     RQHGRLIAREAKLLGFNTVFAPVLDLALPESSTVMRTRVVSANPQDVITYASGFLDGL
                     EAESVLGCGKHFPGLGGGNLDSHHSTPLIERPWETMWNEDIAPFRALAPRLSMIMASH
                     AIYPKASHERRPASISAFWISEILRRKIKYTGLVVSDDMEMGGILTQQRIEDAVIAAV
                     SAGTDVVEICKDPALVLHSYEALLTEAEKSTAFRQKITRAFRRICKLAPPKQLFALPA
                     HSRILGLREKVKVFSDININSFSSSVLQSGGNVYENKNK"
     gene            39619..41127
                     /locus_tag="VFA02_07375"
     CDS             39619..41127
                     /locus_tag="VFA02_07375"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZU09708.1"
                     /translation="MATSEYIRKTTGAGKKSVQAVPSLIASDDRLSGPALASKLMLAM
                     SDVLVISLAFVFVLILKVRFFAGYGAFRSLEIVKTPIYLAFFGWFILSLLFVCRRYGL
                     YNLTRPFSGAHELRLVVQATLTAGLLLCGGLYMAHWDVASRTVVVLLICTSAISLSIW
                     RSGLRVARYRNFEKGVGTRNVVVLGTNYLSYALGEHIASNYRLGYKFIGHIAPSSSPD
                     EHTVVSNVLGHMDRLHQLTRLHFIDEVVIAQALPNEIVISLLEEARELGIDIRAISGL
                     YSDLATNSSIEYLGVYPVTALHRSRSKVISSVFKRVFDIIFSLAALAIALPLMLVVAL
                     AVRLDSPGPIFYISERVGKRGRIFPCFKFRTMVKDAEKKKKELAALNERDGILFKISN
                     DPRITRVGRFLRKYSLDELPQLFNVLRGEMSIVGPRPPIASEVAQYELEHFRRLEVTP
                     GLTGLWQVQARQDSSFDKYIELDTAYVENWSFWLDVKILLRTALVVVRGTGT"
     gene            complement(41175..42377)
                     /gene="alaC"
                     /locus_tag="VFA02_07380"
     CDS             complement(41175..42377)
                     /gene="alaC"
                     /locus_tag="VFA02_07380"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HZU09709.1"
                     /translation="MEEFPRIKRLPAYVFNITAELKNAARKRGEDIIDFGMGNPDGAT
                     PQHIVDKMIEAARKPETHRYSLSRGIPRLRKAICNWYQDRYDVALDPDTEAIVTIGSK
                     EGIAHLCLATLDQGDVVVVPNPSYPIHIYGPVIAGANIHSVAIHDTEEFLAQLERTIP
                     LMYPRPKMMILNFPANPTTQCADLHFFERVVALCRERNIYLVHDLAYADICFDGYKAP
                     SILQVPGAKDIAVEFFTLSKSYNMPGWRVGFMVGNKTLVGALGRIKSYFDYGTFAPIQ
                     VASILALEGPQDCVHEICENYRKRRDVLVNGLNRLGWQVPMPKATMFVWAPVPETYRA
                     LGSLEFSTKLLKDAKVAVSPGIGFGEFGDGHVRFSLIENEERTRQALRGIKQMFLKDG
                     VLATATAV"
     gene            complement(42435..43202)
                     /locus_tag="VFA02_07385"
     CDS             complement(42435..43202)
                     /locus_tag="VFA02_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZU09710.1"
                     /translation="MTYRVVVVDDEPLARRGIVSRLQPYSQFQVIAECANGEDALQVI
                     AEQKPDLIFLDVQMPEMDGFEMLEHLPVEERPAVVFLTAFDQYALRAFQVHATDYLLK
                     PIDDVRFNEAVERVERMLQMERDNTLSERLESLLSDLHARRAPGNAQRFAIRNGRRIF
                     FVTANEVEWIEAQGDYAALHVNGKTHLLREPLHILERRLDPAAFIRIHRSTIVRLDRI
                     AEMQALANRDCLLRLKDGTPLRVSRSYSDRLQAALGG"
     gene            complement(43199..>43750)
                     /locus_tag="VFA02_07390"
     CDS             complement(43199..>43750)
                     /locus_tag="VFA02_07390"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU09711.1"
                     /translation="EHDTATANRMIARLADFLRATLDDDGAYEVPLRDELDITNLYLE
                     IEKTRLCDRLIIVDETDPTVLDAAVPRLLLQPLVENAVRHGIGKRREGGRLFMSTRRD
                     EEDLVIQITNDVMPLNGTKFRNGIGLSNIQTRLKQLYGDAHRFTIDFCEGDVCKVTIR
                     IPFHLYASPAEVESEKPTGSGLP"
BASE COUNT         9774 a        12879 c        11560 g         9537 t
ORIGIN      
        1 gcggggcgaa ggtcgtgaaa acttccggta aagtctgcca gatactcatc gtactgaagc
       61 tcttcacgct ctctccagtt gatctcggca aattcaacgg atttgtggaa gatgatttcg
      121 gccttggatg ttgccagctc aaagccggca taccaggcac cccggtcaga ggtggagaag
      181 cgcgctccca gcggattggg atgcgtgaat gcggcgttga tgatacggta atgcggcacg
      241 ttgaagacca actcgcgctg tgtaattccg gggcgcagat tttcttctgc ttccaggcgc
      301 gcgttggtag cgttgtccaa ttcaaaaata tgttggagat gcccgtcgtt atcagccacg
      361 cgcgtcaaaa cagactccag gttggcgctg tacttcgccg gaatgagacg gtgcgtatca
      421 aaccgccgga tttgcgccaa cggcggcaac gacggatctg gcgggagcgc ttcagccaaa
      481 ggtatttccg gatgcgcctc catctgagca cgcaccgagg ggagcggatt ttccatagca
      541 gcgttttttg cgcgcgtcat tcagaaggta ggataacgaa gttcctgaaa aagatacggc
      601 cccgccacgg ccaaatgaca ggaaccagat tggcggttgc ctactccacc tgttgctggg
      661 ccttgtagcc gtcgcgggcg ataatgtcgt atttcagcgg atgatgcttt tcatcctgca
      721 ttcgcaatgg atgcccctcg ttgtccacca gctcaacacg aatcttgcgc caggtgccgt
      781 ccagcttctg gttggtcggg tggtagctga gcacgtattc gttacggatg gactcattaa
      841 tctgatggaa gatgtcgggc atctctccct cgaagcgcgg gaagaaggcc atgcctccgg
      901 tcatgcgggc aaaggttgtc atctgattgt ctgcctgcag gtagtcaagg ttgcggatgg
      961 ggcccatcat gccgtagctg tcagccagga tgcgcgccgt ttgccccgtg ccaacagaaa
     1021 agatggtcac attcggcgtg gcctttattt tctgcaggac tttgtcgagg gtgattttgc
     1081 tgaaggtgtc gcgtccggag gaaatcagga tgatgtattt ccggccatcc acacgggtca
     1141 gccgatccag agtttcataa agagcgtcga aaaggtttgt ctcctgccat gtcggaatgg
     1201 taagcgagtt cagcgcttcg tataccaggc gcttgtcctg ggtgaagtct gtcagtatct
     1261 gggtgtgcat gtcatacatc accacggcaa tatagtcatc gggcttgagc tgttgcgcga
     1321 aggcatacgc agcattttcc atatcctgaa taaaccacca actgttggcg gcaaattcaa
     1381 ctaaaaggac agcggtaatt ggggcctgaa tacggcggaa gtcggtaatc tgctgcggaa
     1441 cgccgtcctc ataaacgcgg aagttttctt tttgcaggtt gggcacgaac tgatgtgttt
     1501 tttcaagcac tacacccaca tctacattga ctacagggac gttgacccgc aaagaataat
     1561 tttcaagccc gggagggttc ttcaccttcg gctccggcgg gggtggtggg gcctcttcct
     1621 ctttttcctt cttcttagga acggctatag cgcctgtatc gcccgcgggc ccgcctgctt
     1681 ccggggcgtt ctggtcttgc tgttgctgct gattttgctg attctgctgg gtttgctgct
     1741 ggtcctgctg ttgtgcaggg accggcgttt ccgcctgcag gaaacctgcc agcgcgcaga
     1801 agataagggc cttacgaagc gaaatcctca tgggtgactt cattgtcgaa ctccgaagca
     1861 ttctactgca agcgtccttt tgcgggcgct tgttacgaaa ctaaaatcgt ttcgtcagag
     1921 aactggtact ctactgttgt agacgtgatt acccgcctga aagacatcgg ttggctcatc
     1981 ctgtgcacag caacagccct ggggggacag accgtgtccg catcttctcc tctgccggca
     2041 gcgccacctc cttatacagc agggttttat cctctggacc aggtgcatcg cggcctgcat
     2101 ggggtggcct ataccgtttt tgagggtaca aaaccggagc ccatgggggt ggaaatcctc
     2161 ggcgttttgc ataacgccct cggccctggg caggacatga ttctggcacg cctggagggc
     2221 acaaaacccg agtacacagg agtggttgcg ggcatgagcg gcagcccggt ctatgtggat
     2281 ggcaggctgc ttggcgcgct aagttatcgc atcggcatgt tcagcaaaga gcccattgcc
     2341 ggaatcacgc ctatcagcca gatgctggaa gtagcgcacg accatccata tctgatggcc
     2401 tcgaacggca gtggaacagc agtcaagccg gcctcttcgg acgtaataca gaccgatgca
     2461 atccaaccga tggacacccc tctggtcttt acgggattta gccagcaggc ccttgagttc
     2521 tggaaagagc atgctccgcc agccctgggc gcaacagcag tttccggaat cgggggatca
     2581 tcgagtgatg agaagcagcc cgatccaatt gtccccgggt cggcggtaag cgcgctgctg
     2641 atgcgcggcg atcttgaaat cgcggccact tgcactgtta cctatgtaga tccaaaacag
     2701 cttctcgcct gcggccaccc gattacccag ttcgggccgg tttccatgcc gatgaccaag
     2761 gctcaggtgg ttgctacgct gccttctgca tataacgcat tcaaaatcat caatacgacg
     2821 gaaaccgtgg gctcgtttac tgaagaccgc gagtcggcca ttctcggaca cttcggccag
     2881 caagcgaaga tgattccggt gacggtaaac attaccggtg aacccgctcc gcatacgctg
     2941 cacatggagg ccgtagacca gccgcagatc acgccttccg ttgtgatggt tgctgtcttt
     3001 cagggattga tggagcgtaa tgacttcaca gcggaaacaa gctatcgcgt ccgcagcacg
     3061 gtggacattc ctggctatcc ggctgtccat tttaccggtc tggctgcgcc tggagagctg
     3121 cctgccaatc tgaccgcggc gctgatggta ggagagcagt tttcgcgtct gtatgacaat
     3181 gccgcgcgtc aggccccgat tcatcgcgtg aatattgaag tggaagccat cccgaagcgc
     3241 ctgacagcac agcttgagag cgcgcagtcg gctgacacca tcgtacacgc ggggcaaacg
     3301 gtcgtgattc atgcggccat tcgcccatgg cgaggcgaaa tcaggaacat ccgcattcca
     3361 gtcacactgc ctgccgtgct gcctcagggg caagtacgtt tgctggtgag cgatggcagc
     3421 acactcgacc gccttacgca gtcaccttcc cgttttatga attcgcagcc gctagatgtg
     3481 gccgccacca ttgaacagct caacagccag cacgaaaacg accatctcta cgtaacgctg
     3541 cttgatcctt caccgcaggc cgtgcttgac gggcgtacac tgacgaccgt acccgtatca
     3601 ctggccaacg tgttggaacc tatgcgccag aaccagaagc tcacattgaa cggcgaatcg
     3661 gcagtgccgc tggcctccgt tccgattgac agtgttctga atgggcaaca ggtgttgacg
     3721 ctcaaggtgg agtaaaaacc gcagtctacc ttgacgaaga aacaggcgag gcaattcgcc
     3781 gcacctctcc tgcaatccgg acaatttgtt cttcctcgag atacggatgc atgggcaggc
     3841 tcagcacccg gtgccctgcc cgttcggcat ttggaaagct gccgtggcca tgattcatgc
     3901 ccgcgaatac aggctgcaga tgaagcggca ccggataatg gacggcagta ggaattccag
     3961 cctgcttcag cgcactttcc aaagcatcac gctcatctac ttcaattgtg tactgcgcat
     4021 aagcgctggt gtttccatcg cgtatgacag ggaccctgac tgcatcccca agcagcctgt
     4081 cgtacatggc cgccgacttc cggcggagtg caagttcatc atcgaagacg tccagtttcg
     4141 ccagcagaac tgcggcctga attgtgtcca gacgcccatt gattccgata gaaacatgat
     4201 gataacgccg gtcctgccca tgattgcgca gctcaaacat gcgacgggcg agttcatcgt
     4261 cgctggtaaa gcacgctccc ccatcgccat aacagccgag aggtttcgaa gggaagaagc
     4321 tggtgcatcc gatcgtgctg agattacagg actggcggcc tttatatgtc gcgccaaaac
     4381 tctgcgcagc atcttcaatc acggcaatcc catgtttttc cgcaaccgcg ttgatggcat
     4441 caaaatcagc acattgtccg tacaggctga tgggcatgat cgcctttgtg cgaggcgtaa
     4501 tggcagcctc aattttgtct ggagcgatgt tgtatgtatc agggtcaatg tccacaaaga
     4561 ccggagttgc gccgagcagc gcaatcattt ctccggtggc aaagaacgtg aaaggcgagg
     4621 taaccacttc atcgcctgct cctatgccaa gcgccatcaa cgccagcaag agcgcatcgg
     4681 tcccgctggc gcaggtgatg cagtatttcg atcccgtgcg ggcagcaagc ctttcctcga
     4741 gttctaccac ttcaggaccg aggatgaact gtccgtgatc gagcaccgcc tgtatgcggg
     4801 catctatgcc ttgtttcagt ctgcgatatt gcgccttgag gtcaacaaat tgcatatctc
     4861 ctattgtctc gcaagcaagg cgcagatctc ctgtccagtt cccaaaatct gaaatgccgc
     4921 ggcctacttc gtagaccaga agtcgggatc tgctttgacc caggtttccg ccgggtagct
     4981 catgcgtgga tcaaaagcaa aaaggtttgt ctgactcgac tcaatgcacg atgccgtcaa
     5041 gtcttcgatt ctcttcatcc ctgcttctcc tactgctgcc tcaaagtttt ttgattcagc
     5101 ggagaagtgg tccagttcgg agagcgattt cagaggaatg aagaccaggt atactccgcc
     5161 attgtcttgc ccatagacac tctcaaaaac agcccaatgc acatccggaa cccccttctg
     5221 ataaccgttc atatactcct tgaccagagc ttcccattct tttctgtgtc ccgggcgcac
     5281 cacgtagcgg gagatttcaa agtaccgcat tcgcgcgatg tttacgccgg catgatagct
     5341 caggtcttct cgataaacgg ccacattgtc atcgtatccg ctcagcagtt cgccatcggc
     5401 gagggctgca ttgtcaaaag cagccgaaag ctttgcgtcc ttcatcattg ccagattgtc
     5461 cttctcccat gcgtcgaagg agtcgtagcc catgatgaac agtgcccgcg atgggcctga
     5521 aagcgaggtc actgcgaaat agtgtgtggg ccattttgca tcagtcatgg cctgtataaa
     5581 cgcgctttca ctctttacgt gcacagcccc ggccttgcct ggttttatga actcgcgatg
     5641 cacaacaagc acttttggcg gctgcattac accttcagtt ggttgctgtg ccaatgccgt
     5701 cagtccgcat gcggacagac ataatcctgc gaagaattct gtaagtcttt tcacggatgc
     5761 tgctcctgtt ccttaaggtt ttcagaccag gaatgccgag cgtgagggga atcaacaggc
     5821 agcaaacaat tcaggcggga gccgcttcca ttcctggagt gaatgtaacc atactcttcc
     5881 gcacgcttcg gggcaagaag aaatacagcc tgatgttgtt ctcgaagcaa aattcagttt
     5941 tatgtggcat ccccacagaa gatatgccat actgatgtgg cataaccaca ggagtcggta
     6001 atgagcaaat caaaacttga ccttctacaa ggcaccttgg acctgatggt cctacagaca
     6061 ttagcggcca tgggcgcact ccatggatac ggcattgcgc gccgcattga gcagatcagc
     6121 gggaatgaag tcttgctgaa ccagggcacg atttatgcct cgctggtgcg cttacagcag
     6181 cgcggatgga ttgccgcttc gtgggggact tctgagaaca accgcaaagc gaagttctac
     6241 tccatcacta aaaccggaag aaaacagctt gcgcaggatg cggcctattg ggaacggttg
     6301 tcaggtgtca tgggccgggt cctggccatg cagagtgaag gcggcgagca atgatctatg
     6361 gtctccctga agcattgcgc cgcctccgct cttttttccg caaaggcccc gttgaccagg
     6421 aacttgacgc cgaaatggcc tcgcacctgg aaatgtccat ggaagaaaac ctgaagcgcg
     6481 gcatgtctcc cgatgaggcg cggcggcagg cactcattca attcggcgga gtgcagcagg
     6541 cccgcgaaca tcaccgtgaa gcgcgcggtc tgccctggct tgatgtcctg atacaggacc
     6601 tgcgcttcgc cttccgtacc ctgcgtcgcg accgcgggtt caccattgtt gccgtgctga
     6661 ttctcgcgct tggtatcggc gcaaacatcg ccgttttcag cgtggtgaat acgattctgc
     6721 tgcggccctt gccttttcgc gatccacagc gattagtgcg aattgtagaa aaaggagctg
     6781 gcaccaatga gtccggcaga acatacactg cggacgccac ccaggacttc cagcaacaga
     6841 accacacttt ccagtccgta agtggttatt tcgctttctc cagccccgac aatttcaagc
     6901 tcactggaaa cggacagcct ataccggtta caggcattct tgtggctgaa ggattttttc
     6961 aaacgcttgg tgttgatccc gcgcttgggc ggctcttccg gcctgaagaa tttgctaagc
     7021 atgcccaacc tgtggcgcta ttgagttatc cattctggaa acggcagttc cacagcaatc
     7081 gcagcattat tgggcacaca gtaaatttca acaatacttc ggtaacgatt ataggagtat
     7141 tacccgacag cttcgacttc ggctctgtct tttcgccggg agcaaaggtg gacctcttcg
     7201 tgccctacat catggatgac ttccgcgaag atggaaacga tctggcgctg attgggcgtc
     7261 tgaagccggg agtcagtctg tcagctgcac aaagagatgc cgatgaaata ttcccgaagc
     7321 tgcttttcga acataaacat cctgagttca aaccggaata tacgggccag cttacggtcc
     7381 tgaaggatta tgtaagcggt aagctccagc gttctctcat tgtgctctgg tgtgcagtcg
     7441 ggatgatcct gctgatcgcc tgcgtaaatc tctcgaacct gttgcttgcc cgagcagcag
     7501 cgcgcagtaa agaattcgcc atgcgcagcg ccttaggagc gggacgaggc cgactcgtgc
     7561 gccaatggct gaccgagagc ctggtgctat caggcgctgg agcgctgctg gggctgggcc
     7621 ttgcctatac tgtgatttca tatctcgccc atcagggatc acttgctctc ccgcttctta
     7681 gcagcgtgcg tatagatgga acagttttga catggacact tttgaccaca ctcatcgcgg
     7741 cacttctttt tggattggcg cctggactgc gcgtatctcg agtgaacctg caggaagaac
     7801 tgaaggatgg cggccatgga accagtgagg gcagaaagca tgaccacatg cgctcggcgc
     7861 tggtcgtcac cgaagtcgct ctggcatgtg ttctgcttgt aggagcggga ctgttattgc
     7921 gtagcttttt gcacgtactg gatgttgatc ttggtttcga accaggccat gcagccgcca
     7981 tcagcgtgga ttacgacagt ggcggcaatc cggcaaaaca ggctgcgatc tggcaagatg
     8041 tagtaggtcg tgtttccgtg cttccgggag tggaagcagc aggcatctcc gacaatctgc
     8101 cgatgagccg gaacagaggc tgggggattt cagccaaagg cgaaaaacag cagtctgatt
     8161 cgccgtttgt tggcaccttt gtctacatta tttcacccgg ctatctgaaa gcgatgggga
     8221 tgcacctgat ggaaggccgc gatatctcct gggaagacct tgtaaataac cagaatgtag
     8281 tcatcatcaa cgagtctgtg gcgcggcgac tttggccggg acaagacccc attggaagaa
     8341 cagcaagaac aggcggaaca gatgtccgag taatcggcgt aatagccgat gtgcgtgaaa
     8401 gcggcgcgga agacaatgca ggaaatcagg aatatctacc tgccaccagt cagtttggcc
     8461 cagagggagc aaatctggtc gtgcgctcaa agttgcctcc ttcagcactg gctacaagcg
     8521 tcatgcgcac tttgcggcag atcaatccgg ggcaacccgc gacagaattc aagcccattc
     8581 aaacactggt ggaccacgca acatccccaa gacgcttctt tgtgctgctg gtggggacct
     8641 ttgccggact tggccttctg ttagcttcac tggggatcta cggcgtcatt tcgtattccg
     8701 tcacccggca gacccaggaa attggcatcc ggatagcact gggcgcaacc cagggacatg
     8761 tgctgcgtac cgtaatctgg aggacattgc ggctggcgct cattggaatt gcggcgggaa
     8821 ccatcgcatc cttcgccgtt gcgcggatga ttgcctcatt gctattcggc accgtgccaa
     8881 ctgatccaat caccttcaca ggagtggttg tgctgctcac gctggttgct ctcgttgcag
     8941 gcttgattcc ggcgcgcagg gccgcacgga ttgacccgat gattgctctg cgaaccaact
     9001 aagacatttt cacagattct gcacaactcc atttgtactg tgcagtgtcg ccgactccct
     9061 gttggtcgcg gcaactgtag tttcgcggca tggttcagac tatctgctat agtgtagcgg
     9121 gactggcgca ggacaccaac gtcatgaact acagcagact gaacagattc ccattatttt
     9181 tctcaagcag gtttacaacc tgaatgagcg cggtttgctg tgtttccgcc gtgctcattt
     9241 gtgtggaaac cttggccaca tccgtctgca acaggtctgt ctgtgctgct gtaatctgcg
     9301 tcccctgcat ctgggtataa ttttgtgccg cttgcaggcg ggtaatggaa ttgtcaagga
     9361 caacgcgctg ctggcttaca tagttcagcg ccgaattcag cgccgtggca tcagccacgc
     9421 ttgcagcact cggcgcgccg gaagaaaatt gcgcaaccag attgttgagg acgcctaata
     9481 catcattcga cggattgtta aaaattgcat tgcccggaac gttggtctgt atctgctgcc
     9541 cattcggggt catgacgtag gaaacgacgg aatcgccatt gtaggtaacc gtgtttggcg
     9601 tgaccgacgt gttcagtgaa aacggggccg tattcccttt acttccggaa aacagatacg
     9661 tgcccatata agttgtgttc gcaagcgaaa caacttcgtc acggatgcca gaaagttgcg
     9721 atgcaatgga ttgcagatcg cttgcattca gagttccgtt atttccctgc atggcaaggg
     9781 aaacggcctg cgtcagttgc gacactacgc tgccgagggt cgagtccgct acctgcaaac
     9841 gtccaacgac agaagtttcg gtctgcgaga aactgtcgtt catctgcaat tgcgaagcga
     9901 gcagcacatt ttctccggct gcaaccggat cgtcggccag agaattgaca cgctttccgc
     9961 ttgaaagctc atttgtaagc tgctgctgga gagcggaggc ctggtcaagc gaggtaatcg
    10021 cattattcag atagagcgga tcgaatctca caatatcttc tccttatgca ctctatgaaa
    10081 ccgacgtctc aacgccaaga ttcaaggccg aagccatgac tgtatccagg atggtgaata
    10141 cctttgatgc cgcctgatat gtctgctgaa ggttttcaag cagcgcggcc tgatcgttca
    10201 ggttgacagc cgacagagaa tcgcgctgcg tcgtaagctg ggtcaacgaa gcctgcagcg
    10261 ctgtattttc cgtcgagacc tgagaaacca gactgccgag agaggacaca aagcctgaat
    10321 agtaactcga cgccgttgtt cctcctgcaa tcgcctggtt ttgaagctgt agcatgctca
    10381 ccagattgga cgtgtctgaa gggccattgc cgctggcagc agcagcaatc tgcgagccgg
    10441 atgaaatggc gacggatatc tggctggcgg cccccgcaga agcagctggc agattaaaaa
    10501 tcagaccacc ggggtttccg tttgcatccg tgcctgcctg atttgccaca ttcagtgccg
    10561 ttccaaaatc gtaggccagt gtatccaacg cactctgcat ttgcggaatg tcctggtcac
    10621 gaaccgtcag aataccgcca agctgcccac ccgcgccggc cagacccgac gtgatgtcgt
    10681 tgccgtgcgc atcagaaata tgcgtaactc caccggaaag cgcggtgttt aatgaaaatg
    10741 cctgatcttt gacgaccagc ggcgccccgt tcgcggtcgt aatcgtcaac ccgttgtctt
    10801 ccgtctgtat ctgatgaatg ccaacaagtt gtgaaagctg ctcgatatca tactggcgct
    10861 ggtcttccat ctggccagca tcgccgttgg gtgacatctc agctatcttc tggttcaacc
    10921 cggcaatcgt ctgagtcagt gaattcacct gcgaaacgat cgtgtccgcc tgatggtcca
    10981 ccgaattctg ctgactggac aactgcgagg acgctgcatg aaatgcattc accagggtgg
    11041 ttgccgccgt gaggactgac tggcgcagag atgggtccga cgggtttgac tgtaattgcg
    11101 aaagcgcgct gaaaaatccg gtgatgcttt gcccgatatc aagggaagaa gacgaggtgg
    11161 atgtagccga actgaaaata ctctgcacct ggtcgagagc ggtgtaacgc gcatttgtgt
    11221 cctgctgaag ctgcgtctgc tgctgaatgc gctgttccag caccaggtcg cgctgcgaag
    11281 acggaccagt catcgtggcg ccttgcccat agtccactcc gccaatattg actgtcacgt
    11341 tctcctgcca ctgcggaagc tccgtagtgt atccaggcgt gcttgcgctc gacacattgt
    11401 tggctatcac attgatggca gattgcgccg cctccaatgc tccggtcgca atgctgaatg
    11461 ccgagtttaa cgttgccatc cccgagtcct cgctgttccg gtttcgcccc gcagaatgcg
    11521 gaggttctcg ctggtttgct ccagcagctt gctgaatacc gcccgctgcg ccagagcgcg
    11581 gctgaattct tcctgcgaac cagccgcctt aacactgtcg caatcagaca acacttcgcc
    11641 gatggacgcc gcatctgccc ttttcaaagc ctcaatcgca cgctcaaaaa gggcgacaat
    11701 gggtgtctga gaaagaccca cccttgacat gaaaatttcc cgattacttg ccgatcattt
    11761 gccgcacgag cttctcggcc acatcttcag gacttacgtt gtatgttccg ttgtggatgg
    11821 cctgctgtac cgccgccact ttgtccatac gaacatccgc ggcctgcatg gccttgtttg
    11881 ccagagcagc cgccgcgctg acatcagcgc tgtcctgcgc cgttgcgccc gtgccaggtg
    11941 aagacacaga ggtctgccgc tgcacaggag agatccgcga agtctctgta actcctgttg
    12001 cctccgtcaa attgtgcagg ccatcgttga tgttcatcgt tcttgctcct caccgtgcct
    12061 tatcggcaaa acagaactaa attatagtgt ctgtccattt ccgattctgt gagaattttc
    12121 atcaacctgc gctgtgctat ggaaatgccg gagaatctgc gtcgcgatcc caaacccgcc
    12181 atgctgtgag atgcccctgg ccatcgcttc ggcgccgtag ctcatcagcg tgtcagcgct
    12241 tccagttgcg ctgtccccgt cagtaaacag gccatcttcc tctaatggct tgagaaattc
    12301 cttcatcagg ctggcctcaa attcatgcgc cgcggattcc agccgcggat tcggctttat
    12361 ctcgccactt acagctgtag agctcgaaaa cgatacagag ttcataacac ctccagttca
    12421 gcttccaaag caccggcctc tttcatggcc tgcaaaatcg agatgatgtc acgcgccgac
    12481 gcaccaatct gctgcaaatt ccggacaagc tcatccactg tggctccctg tttcaattcg
    12541 atacggttca ctggctggtc ctgcgtttga atggtcgttt gggggacaac ctgtgttgtc
    12601 ccttgtgaaa atggcgcagg ctgtgagacc tgatattccg tgactacatt gaccgacaac
    12661 ccaccatgca gaatcgaaac cggctgcagg cgcacgtccc caccaatcac caccgtcccg
    12721 gtacgctcat tcacaaccac cttcgcgcgc ggatacgtct cgacctcgac cgattccacc
    12781 tgcgccagca gagaaggcac atcatcggaa gactgcacgg ccaatgtaat gcggcggctg
    12841 tctacggcat gggccagcgg cctcgccatc tctttattga tcgccgccgc catcatctcg
    12901 gccgtgcgga agtccgcatc attcagcaga agagaaagtg tcttcattcg tgaaagatca
    12961 agcggcacag cccgctctac cattgcgcca tcgggaacgc ggccggttgt agggtggtta
    13021 tactgcttca gatttccatt ggcgcctact gcgtagccgc cgaggaccag aggaccttgc
    13081 gcttcggcgt agatctgtcc gtcaggccca tagagcgggg tcagcagcag aagccctcct
    13141 tcgaggcttc gggcatctcc ggccgaggag accgttacat caatcttatt tcccggacgc
    13201 gcaaaggcag gcagcgtggc cgaaataaac accgccgcca tgttctgcgt acgcatctgc
    13261 gaaagctgcg gcgactgctg catgttcact cccatgcgct ggagcgtgga aaccagggtc
    13321 tgcaagggaa agaccgtctg ctgactgtcg cctgtgccgc gcagccccac cacaatgcca
    13381 tagcccacca gctggttgtc gcgcacacct tcgacggtgg caacgtcttt cacccgggcg
    13441 agcttcaccc gctcgtacgg ccatgctgtc agcggccata gcagtaacaa gagataaccc
    13501 aatctggcgc agcgcataaa tccatctcct aaaaaatcaa tagcttctgc aagaaacgca
    13561 cggctgcatt gggccggtag gtgtaatcgt tgatgatgcc tttgcccacg acttcaacct
    13621 ccagactggt aatagcggtg gaaagcacgc ggttttgcgc atccacatct tccggacgaa
    13681 caaggccccg cattttgata agctgcgtct gttgattgaa ggtcaattga cgcaccgcct
    13741 ggacgaccaa catcccgttt ggaagcacat ccacaacttc gccacccagc gtcgtgctca
    13801 ggctggaatt tgtgacgctc tcgccctgcg cgttcaaacc ggaggcagat gcctggttga
    13861 caaggttatt aagcgcattg ccggctgaaa gctttccgaa aagtgatgag acctgggaac
    13921 tggcgttgga tgcacgtgag ttcttgactg tgccgtccgt cgaagcagcc agactctcgg
    13981 aaacgacgat tgcaattgga tcgtgaacat gacacgcctt cacatctgct gaaaggcgcg
    14041 tcaaccttcc atccggcgtc cagattgcgc cgggagaatg ctcctcggcc gcattctctg
    14101 cgcggacacg ctcaatatat gcattcagtg ctatgtccgg aggtgttggt ttagactgct
    14161 tttccggctt gtgagttgcc gcgaatgcga caccacataa tacgaaacat aaaacagccc
    14221 cagtccgcgt ttgctttctc atcattgccc gttccacttt gccttaccga taagctcgac
    14281 ctggcccagc cctcgtattt ttccttcgag cacggcgcca gacccaatgc gcacgcgaat
    14341 gctgtccccg gtgcgtcctg gctcgagcgc cgttgcggtc agaacgatcc ttgctccgtc
    14401 ttcattcttc cggagaatga cctttgcgcc ggcctccaca acaggcgtgg cctgtaatgc
    14461 tgttttcgct tcgatttctg catttgcctg cgagatttct tcggtcaccc agggccgctc
    14521 aggatgggcg caatctgcca caacggccca atagcggtgc agcacgccgt caaaaatata
    14581 gcggaccacc acactgcctg gaccgcgcgc actgcattct gcaccaaatg caggcgatac
    14641 aagcaacaaa aggacccatc cgagatgccg catcgcacgc taccgtgtca tgttgttgac
    14701 ctgactatac atatcatcgg cggcgcgaat cactttggaa ttgctttcat aagcgcgctg
    14761 cgccagcacc atctgcacaa actcagtcac aacatccaca tttgagtttt caagatagcc
    14821 ctgctgtaat gtgcccagtc cctgtgtgcc tccaggtgta tccagaacgg cctggccgga
    14881 ggaaagcgtg gcctcaaaca aattattgcc aatgctgttc agtccttcag gattggcgaa
    14941 tgtggcaagc tggatctgcc caagctgggt cgggttctgt tgcccgggaa gagttgcgtt
    15001 cacaacgcca tactgtgtga tggcaatcga agtcgcattc ggcggaatgg taatacttgg
    15061 aatcagcggg tcgccgtcaa tcgtgacgat ctggccctga ttattcagat gaaagctgcc
    15121 ggcacgcgta taagcaatgg ttccgtccgg gcgctgaatc tgaaaaaagc cgttgccctg
    15181 gatcgcgaga tccagcggat tgcccgtctg gttcagatcg ccctgcgtca tgatgacctc
    15241 tgtcgccgcc gattttgtgc ccagccccac ctgaagtccg gcagagacgg tctgctgact
    15301 ctgcgccgct cccggagtca caaggttctg gtagatcatg tcctgaaact ggagcctgcg
    15361 ctggcgaaaa cccgaagtgg acgagttcgc caggttattg gcaatggtgt cgagattcaa
    15421 ttgctgtgcg ctcatgccgc tcgccgccgt atataacgcc cggatcattt ctgcttttct
    15481 ccttgcttaa actttgggaa gatcttcgct tgctgtcttg tcgaagttca cataaaagct
    15541 ggacaacgct ttttgcatca tctctgcctg acgctgcacc agaaccaggt ccagcgtgcc
    15601 ctgaatgacg tcctcattcg acgtttcgag cgcaccctga tggatggctg ccgctgtcac
    15661 cgttggtttt gccgctgccg taaccgcata ccggttggcc ccttcaggga ccaacgccga
    15721 agccggaaca tcaaagactc ccagcttacc tgcaactgcg ccgccaacag aaaggacgcc
    15781 atctacgcca acgactatgt ctcctgtggg gacctgaatg ggtttatttt gcgcgttgag
    15841 cacaggttcg cccgatgcgg tcaccagagt gccattgagc gcgcggcgaa aggagccatc
    15901 gcgcgtatag cgaaccccct gctttgtctg gatggcaaag aaaccctgcc cttcaatggc
    15961 aaggtctaaa ggattgccgg tatgcgtaag cgcgccctgc cccagataaa ggtgattgcc
    16021 acccagcaca ccgtagttat tgacaatctg attgacctga gagttgagcg cctccggacc
    16081 gacaatggca ttgcgaaaat actctcgctg cgcccggaag ccgctggtgt tggcattcgc
    16141 cagattactg gccgctgcat ccaatgcttc ggaacgggcc aaccatccgg aaaacgccgc
    16201 gtaatagccg ctgtccactt cgtcctctcc ttcgtaaaca gatattgcag caggaggacc
    16261 aaagaaaggc ggagcagatt agaaaaatct cattgaatag attcacagac aatactgatg
    16321 ttgaccgcat gctcttcaaa cctgtagctg cggtcaatcc gaaactcagg ctgctgcata
    16381 tgcaactggt aattcgtccc tgtcacgacg gcgggagtag aaagcatgca gccggaagcc
    16441 agcacgggat gctttccttt ccatgctcct gccaccatat tgcacacctc gccaatcgca
    16501 tcttttacca tgtcgtccag ttccgcagga cgaacgccga ccatgcactc agccatgcgc
    16561 agcgccgcat ccattccgca gcgaagcgtg catgcgccgc tcatcgcacc cgccagtcca
    16621 ataactgccg taatagtttc gttatttcca aagacatatt cttccgcagg ggcgcagttt
    16681 gcacccatca ttaaacgaaa aacttcctcc actgcgctgt ccaggtcctg cgcatgagcg
    16741 ctctcctgga gcagctcccg ggccggcgta gccatcatgc ttctccaatc agtagttgaa
    16801 attgaacaga ctaaacgagt tccagcaggg gcaaaacacg gtccttgacc tgctcgggag
    16861 taaaaggctt acgaatgtag ccctgcgcgc ctgcagctat ggctgccttc acatgctctt
    16921 cgctgccttc ggtcgtgatc atcactacag gcacatcggg agcaagatcc tgtgcgcgca
    16981 tctgccgcag aaactcaagc ccatccatca tcggcatatt gatgtcggaa agaataagct
    17041 gtacctgttc gcttttgagg acatcaaggg cctctgcgcc gctggacgcc tcatagacct
    17101 tgttcaggga cagccctgcc tggcgcaggg cgcgctccac aatctttcgc atcaccgaag
    17161 aatcatccac gataagggcg ttaatatcgc tcatcagcac tccttgctca atgctcttat
    17221 cggcagcttt cttctccact agagcaccat ctgaactctg tggaaaatga gcagaccatg
    17281 tcttcaatgt ccccggcagc cggaagtgaa tgcttttgcc cggcagacgg agattttttc
    17341 catagcctga gccagaaata attgtggacc ctcaccagac gcgcctcggc cggcctcgac
    17401 tcttcgacca tggcggtcat cgcatgagcg gccccgcgca ggctcgtggt ctcaagcagg
    17461 cgcacccggg ggaaaggagc acgcaacagc gcgccatgcg cgcgctcgaa ttcagaatcg
    17521 cgtaatatcc ccgtctcaca cttcagtccg gtcatgcgct caagcgccaa agcaatgcag
    17581 ggatcgggcc tgctctcgaa ggcgacgtac agaagactgc gcccggcaac acgcaaaggg
    17641 agtgctccaa aactatcggc cagcagacgc ggcaatgccg gggccatggc tgctgcatga
    17701 aatatgtctg cggacaagac cggacagccc cactgcgccc caagcgcccg gaccagttgc
    17761 tcctgctcca ctgctttctg ccggaccagc cattctccca gccgcccgga gccggccttc
    17821 ttctgtgctt cgagcgcacg cttcaattgt tcctgcgtga tatgcccttt tgaaaacagc
    17881 agcaacccca gcgggacgcg gtgctgatgc atcgccggcg agagattgcc atcacgcgct
    17941 tcacgaagca ccgcagcttc aatcatctcc cgcgcgcaga ccgtggaaca gacccacttg
    18001 ccttcaaaca ccggcacttc acggcttcgc cacaggcgca gccatccact tttgcagttt
    18061 ggattcccgc actgcggcaa aaaaggagat gcaatacttc caggccgctc gcctctttcc
    18121 gcacgtttca tcagaacagg catcgtatgc tctccaggga cttgcgaggt tcagcaactt
    18181 cggtgacccg cagcccaaaa tttccattca ccaggacgac ctctccgcgc gccacaatct
    18241 tgtcgcctac aatcaggtcc accgggtcag tcacatgccg ttccagttgc accacgtcac
    18301 cgggccccag ttccagcacc tcacccaggg gcatttcgcg cgagccaaaa cgcaatgagg
    18361 catccagttc aatatccagc agaagatcaa ctcctcgagg gttgaacgct tctttcattt
    18421 ttggtttttc cgttctgtca gcaaaaccga tgggtacaat gacctctccg aaacgaagct
    18481 cataaaaagc aaacttctct ccggcgggaa atggggcttc gcggacagaa gcgatggaca
    18541 ccgtcgctcc agatgttttc cccaccgcct ttgccgtccg ctctgctacc tgccggagca
    18601 ttccttccca aagctgtgca tcacgccttg ggtccatgcc gtccgcagtc acgcgcgcag
    18661 ccatcagcaa ttccgccagt ttttccgcac tcaccagcac ccagaacacg ccatgcagac
    18721 ctccgcccac ttccaactgg aaacagagtg ccttttccgg ttctggcatg gccgcagcct
    18781 gggccagttt caccattccg gccagttcct gactgaatcc catcgggact tccgtcagcc
    18841 acgcatcaag aaatgctttc attgtcttct cccgcgtatt taaatccttc ttgccgcagc
    18901 cggcgtagtg cgcgccaggt gccctgcgcg ctgcccttcc acctgcaccg gatgcgcttc
    18961 aaacagggga atgcccgcaa tgcggagttc ggcaggatga gaggccatca gcggcagttt
    19021 caatacctgg ccctcacgca gcggctccaa ttgccgccct tccaggcgca ccggaggcaa
    19081 ctgcaatgag gcccccacct ttaccagccg gacccgctcc tgcatgcgct gccgcgtttc
    19141 cgccgagtgg cgatgctggc ggctccagtc gccggtcagg cgtcgcagga cagtattcga
    19201 aactaccgcg ggaaaggcca gattgagcat tccgctcgcg cccggcatct ggatctcaaa
    19261 gctcaggcac aatgtttttt ccgagacagg cataatacgc gcgatctggg tgtgcaattg
    19321 acgcctctca aaggaaaagc tcaaccccac aggttgccat gctgtgctta gttcgcggca
    19381 gatcacctcg gccacactgg tcaaaataga ttcttcaatg tcggtcagct ctcgcacttt
    19441 gtccggcctg ccctctccac cgagcagcag atcaatcatc ggaggcgcaa gcgcgagatc
    19501 aagctgaagc accgacaacg cgcgcagagg ctccagcctt accgaactca cataggccag
    19561 atcaggaatg cgcatcataa attcgttaaa gggcatctgc tcggcagaaa ccaggttcac
    19621 gcgaaaacgg ctgcgcagcc agacgcccag attgtgcgta aggttacgcg cgaagatatc
    19681 gttcagcagg ctaatggcgc gaagctgctc attgctgatt tgtcccgcgc gcgaaaagct
    19741 atagggctgt acccttgagc gggtctcctg cgaggccgct ttggaccgtg cccgcgccgc
    19801 ctggaacagt gcgtcaattt catcctgctt caggcttctt tctacctttc ttgttccttc
    19861 gccttccatc acttctcctg tgctctcttt gcagcaagat ctgccagcat ggcgcgcaat
    19921 cgcaatacgg ctgaagcatg gatctgcgag accctggact ctaccacgcc gagcgtcagt
    19981 cctatctctt tcatggtcat ttcctcgtag taatagagcg tcatcaccag acgctctctt
    20041 tccggcagct tgcggatggc ttcggccagg cgctcccgca actctccttc caggcagcga
    20101 aacaacggat catcttcctg gcggtttggc acatacgcaa gctcctcttc gcccgaatct
    20161 tctgaccgct ccatgtgcag cgttccaatc tcaagtccct ttagctcgcc aagtaactgg
    20221 tgataatcgc caagctccat gccgagctca gcggcaatct cattttctga aggtgtgcgt
    20281 cccaggcgcg ccgtcaacac gcgaatcgcc tgttccacgg cccttccctt gcgccgcaac
    20341 tcgcgcggac tccagtcaag cgtccgcagg ctgtccagaa tcgcgccgcg aatccggaac
    20401 tgcgcatagc tgcggaattg tacttttttt gccggatcga atttctgaaa cgcatccatc
    20461 aacccaagca cgcctgcgct gaccagctct tccatctcga catgctgcgg cagacgctca
    20521 tggatacgcc gcgcaatgta gcgtacaacc ggaagatgct ccagcatcat gcgctcctgc
    20581 tccggagtga atctcgcagc atttccatca cgcaccggcg tcttcttcgg cgcgctcttt
    20641 ttctgctttt cttcttccgt tccggcaagc gaaagcattg ccgccttccg cctcatctct
    20701 tcttcggtaa atggccgatt cgccatggct cccaggttcc gcatttccaa ctcatccatt
    20761 ctttttctca gcgcactgtt ccaactgacc gcacgcgcac atccggggga atctccaaag
    20821 gcgaaagcac tacaaccttt ggcagaacag gctccatcca acggcgtaaa taaaagcgcg
    20881 ccggactcga acagagaagc acggggattg ccagattcgg ctgctgggcg attagctgtt
    20941 tcacagaatc taccagccgc ttaaaccaga caacgccgcg ggggcgccca tccgagagaa
    21001 gcaacgcacc tgtctcgccg gaaaacgccg ctgccatctc ttcatccagc gctggatcaa
    21061 gctgcaggat gcggagcgtg ccgtcctctt ccagcaacgg ctgcacaata cgccgcccca
    21121 tggcctgtcg cactgcttcc accagctgcg gcaggttcct gttcacctgc gcagcatcaa
    21181 gcaggacttc cagaatcgtc ccgaggtcac gaatcggcac ctgctcgcgc agtaactgct
    21241 ggaggacctt ctgcacctcg cccagagcca gcaccttcgg caccagttct tcaaccagcc
    21301 tgggatgcga ttcattcagc gcatcgagca aacgtttggt ttcggcccgt cccaacaatt
    21361 catgtgcatg ttgcctcacc atctctgcca gatgtgtggt gattgccgtc acgccatcca
    21421 caaccgagta acccgccgca atcgcctggt tttccaaagc gggagagatc cagcgggcgg
    21481 aagaatggaa ggccggctcc acagtctctt ttcccggaac gggctgggca atccttccgg
    21541 tctcgacggc tagcaaagcg ttgttttcaa tttgccagcg gccgatttct atcccgcgca
    21601 gcgagaatac atactcccgc ggacgaagac ggagattgtc cgaaatatga atagccggaa
    21661 ccacaaagcc caattcggaa gcaagctgcc tgcgcaacgc ccggacgcgt gccagcatct
    21721 ggccgccctg cttttcatct acaaatggaa tcaactggaa gccgatctcc aggctcagct
    21781 catccatctt cagcagcgag gcaatgtttt ccgtctggac ggccgccttt tgcttttgct
    21841 ccggccctgc ggctgcttca ctctgctgct tctttgaaac acgccatccg atatatccaa
    21901 gtgtggccgc aggcagtaga aaggccagtt tcggcagccc gggcacaagt cccagcagag
    21961 ccagtacgcc gctggcgata tagagcgtcg ctcccttacg cagcagttga gcaccgagtt
    22021 ctgttgtcaa tcctgatgcg gtgttcgcgc gcgtcaatac cattccgccg gcaaccgaaa
    22081 ccagcaacga cggaatcagc gtcaccagcc catcgcctac tgtcagcacg gtataagtac
    22141 gcgcagcctc ggaaatcgcg gctccttgct gaagcacccc aatcagaagc cctgccacaa
    22201 tattgatggc cgtaatcaga atggtcgcca gcgcatcgcg ctggttgaat cgggccgcgc
    22261 cgtccatagc gccgtagaat tccgcctcct gcgcaatggc ctgacggcgt tttcttgccg
    22321 cagcttcatc aatcagtccg gcattcaggt cagcatcaat ggccatctgc ttgcctggaa
    22381 gcgcatcgag cgtaaagcgc gcggtcacct ctgccgtgcg cactgccccg tggctcacca
    22441 ccagaaactg gatcgcaacc agcgccagaa acaacacaaa gccgactagg taatttccgc
    22501 cgactacgaa ctgcccgaag gcctcaatca cgctgcccgc agccgctgtg ccttcatggc
    22561 catgcagcag aatgcggcgg ctggacgcaa tattcagcga aagccggaag agcgtcaaca
    22621 acaacagcag gctgggaaat acagaaaaat ccaccgcccg acgcaactgc actgcggaaa
    22681 gaaagaccag taccgaggcc gagaatgaac aggccagcaa cagatcaagc acggccgccg
    22741 gaacaggcac cagcatgaca aaaagaatgc tgattgctgc tacaggcagc cacaggtctt
    22801 tcccgaagcc tgcgattcgc gcaaggacac cctgccctgt tgccggcttt gccgccgcag
    22861 gaacaacaac tgaagcgctc atgctttctc ctttgctttc tgtgccgcgg tccgctcacg
    22921 ccgtatccgc tcttccactt gtttgcgata aagccacgcc agaatcgcag ccacggccgc
    22981 atataaatcc accggaatag actggccttc ctcaaccgag cggtacagcg aacgggccag
    23041 cggcgggttc tccacaatcg gaacacctgc ccagcgcgct tcggccttga tctgctcggc
    23101 gagcaaattt cgccctttgg ccaggacttt cggggcctcc atcgtttcaa aatcaaaact
    23161 cagggcaacg gcatagtgcg tggggtttgt aatcactaca gttgcttttt tcacatcggc
    23221 ctttaatttc ctgcgccgca tctgccgttg cagaccacga atgcggctac gcacctgggg
    23281 actgccttcg ctttccttca tttcctcgcg caattcctgc ttactcatgc gcagcctgcc
    23341 ttcccgcgcc cgccattgca cgatgtaatc cagggccgac caggcaaaga gcacccacgc
    23401 agaatcaagc aatagcgtgt acgccgctga aaacatgcgg ggaagatgtt cgacgctcat
    23461 cggaggaagc gcgagctggc cttcgatttg ccggaatgca aaaaacagaa gaacggcgac
    23521 cggcagtaac gacttcagga gccgcgagct tccctgaagc gaaaacatct gcctgatact
    23581 tgtcgctgga tggatgcgcg aaagcttcgg ctgcaaatgc tgcgcgtgaa atcggacgcc
    23641 tcctccctgc gccatccctg ccagcaatgc gcccgtggct gccgtcccac acaacaggac
    23701 gagaggcgaa agcgaaacaa agatgacatg acgcaaaacc agcgcctgtt gcatgaagcc
    23761 cgcatcattc cacgctgacg gagaagccac agcgagcatc tgcgtcatcg ttccagacca
    23821 caacgatgtc cagtgcggag cagccatgcc caaagccagt acgcccgaca acatggcgca
    23881 tgcggccgtc cactcgcggc tgtaaacccg gtctccctgc tcctgcgcct tttgccttcg
    23941 cctgggagtt gccttttctg tgcgctcgcc gctcatccgt gcgtcactcc ctgccgcaac
    24001 agaccttctg cgacatccag caattttgtg aagcgggcct cgataaaacg cggccacagt
    24061 gccagcgatc cgagcagcag cacataaccc gtaatcgttt ttgccggaac ggtaaccgcc
    24121 atcaccggaa gctgcggcga cattttcccg atgagcgcca ccgcaatctc aaccaacatg
    24181 gtggccgcca gtacaggagc tgcaagctgc aatgccgcaa agaacacccc gcccatcatc
    24241 cgcacaagga ccagagcgcc ttcgccgttt aggaaagccc cacccagagg agcagctaca
    24301 taactcctca tcagcgctgc cagtatcgtc cggtccaggc ccgcggcaat cagcaccagg
    24361 actcccatga gggaaaacat ctgggacatt aaaggtgttt ctacttgcga attcggatca
    24421 agcagatttg ccagcgagaa gctgaactgc actcctaaaa cctgtccggc aaaaagcagg
    24481 atttcgtcca tcagagtcaa cgagaagcca aacaccagcc caatgctcag ctcgccaagt
    24541 acggccgaaa ttcccaaccc ggcgtgggcc atcggcaacg cagctacagc aggcgcaagc
    24601 aggacgccag tggcaatcac gaagatggcc ttcacctgct tcggcaatgc ctgcgatgaa
    24661 aaaaagggtg cggccacaac cattccgccc aggcggacaa gcaccagcac gagggccgac
    24721 agataatgcg cccagtccgt atttccgcgt gcgaatagct cttccatggt cagccgatat
    24781 agcggtgaag atctcccaaa agcacgacag tgaaatagac cagcctgcgc agaaaccagg
    24841 gcagcccaat caacaaggcc acggccacaa tcacgatgcg tgggaccgta cttaacgtct
    24901 ggtcctgaag cgacgtcagc gtttgaaaca gactgataag gaaacttgtt acgctggcca
    24961 gcaccagcac tggagcgctg acaatcaacg cctctttgag caaatacctc atcagctctg
    25021 ctatctggtc cggccccatc agaagctcct cagcagagaa ccggccagca ggttccatcc
    25081 gtctaccatc acaaacagca gaatctttaa tggcgtggag accacgaccg gtggcagttg
    25141 aaacattcca atcgaagtcg tgatggaggc cacgaccatg tctaccaata aaaacggaag
    25201 aaacagaaca gcgccaatca cgaagcctgc tttcaattca gaaagcatgt acgcgggcac
    25261 aaccacgcgc atcggcagat ctgccggtgt tttcggacgc tccgttgcag ccgccgatgt
    25321 gaacaatgcc aggtcttttt cgcgcgcgta cttcagcatg aattgcttgg gaggtcctat
    25381 tccgcgctgg atggcctcca tgcctgagat ctgcccctgg cgaaaaggct cgactgcctg
    25441 ccggtctacg gcactcaata ccggctgcat cagaaaccag gtcatcatca gtcccagccc
    25501 catcagcacc tgattactcg gagctgtctg cgtccctaat gcctgccgca gaaaatgaaa
    25561 gaccaccaac agacgcacca tgggcgtcat cgaaagcagg atggcgggaa gcagcgtaat
    25621 cagcgtaagc gcaaaaacaa tcgtccacgg aacgccgttg ttgtttatca ggtgctgctg
    25681 cgaaccaggc aaggacagca gctttggcgc ttttgcgaag agaagcagca tcgcgttcat
    25741 gctttctccg ccttgcgtcg cttctcttcc ggtcgaatct ccgtcataga aacaatcgtg
    25801 tctgcgccag tagccaccag aaacttatgt ccattgcagt ccaccaggaa cagcatcttc
    25861 ttcgggccaa gtgacaagcg ttcttcgatg cgcagaacac cgttcacact acccacttgc
    25921 tttttcaaag aatgaaggaa tgccgataca actccaccca acctgccggg aacagctgct
    25981 tgtgtcattg ctgtcatttc gtctctcccc tcaagcaagc cgcgttaccc gtacacccag
    26041 cttctggtcc acaacctcga attccgtcca caccagttgc acgtcgccgg cccacagcgg
    26101 aagatcttcc gtatgcggcc agatggtttc cacgatttgt cctttttcaa gcgacagaag
    26161 gctttgcacg cggaaagacg gcaccggtac gcgcaggtcg agttgcagag gaagctttga
    26221 agccaaagct gcaagcgagg tctcgtctac cggcttttcc aggaccacag ggacctgaga
    26281 tgaatcagca gccactgtgg tctggccctc agccgaggta gaaatatcgg tcgtcatgcc
    26341 acggagaatt gcaagcggct gaccggaaac aggacaagaa gattagagga tccttagaaa
    26401 agggctagag ttacagtgca agctgtcgaa catgcgcggc acgcacgtca ttcgccgtaa
    26461 acaacgaaaa gaagggggcc tccttcgcag ccgcttccac cgcggcgcgc ccgccggcct
    26521 cttcgcgttc caccaggcag gccacaccaa tgactttcat tccggcttcg cgcgcagctt
    26581 caattgctgt gatcgtagag gccccggtcg tgcagacgtc gtccacgata aagaccggcg
    26641 catcctgttc cagaaatcct tcaatccgcc gcccggtgcc gtgtgccttt tctgtcttgc
    26701 ggacgaggaa tccatgaatg aatggcgagc catggtgctc ctgcgcatac cacgcacttg
    26761 ccgtcgccag atttgaaaca atcggatcag caccaagcgt caatcccccc acggccgcag
    26821 gctgtagctt gtgctcccgc aacaattcga gcagcgccaa cccggtaagc cttccgcctt
    26881 ctgcatggag cgtggtcagg cggcagtcaa tgtagtaatc gctcgtcccg ccggaggaca
    26941 gccggaactc acccagcttg aaagaaatgc gggccaacaa tttaagaagc gcatcgcggt
    27001 aattagtcat cacaaacgat tgtagatgta gactgcctac gaggtgaaag agccatgaca
    27061 tttcttgaag cgttgcggat tgaatttgac gatgaggtaa acaagacgcg caaaattctg
    27121 gaccgtgtgc cggaaaagag ccttgcgtgg aagccccacg aaaagtccat gacactcggg
    27181 cggctctccg gccatgttgc cgaactcccc tcccgccctg ccgctatttc gcaggacata
    27241 ttcacccggc aagccaacta cgtaccgttt gcagctactt ctcatgtcga gctgatgcag
    27301 cggttcgagg aaagcgtaag cgaaggtcgc atggccctcc aacaatttac cgaagcagac
    27361 atggcaaaac cctggacgct gaaactgggc gacaaggtca ttctggaaat gcccaaatgg
    27421 acggccctcc gcgccgttgt gatgaaccac atgattcatc accgtgcgca gcttggggtg
    27481 tttctccggt tgctggacgt cccggtccca ggaatgttcg gcccttcggc agacgagaag
    27541 ctgtaaatct ccatgttccc gcgcaacgct ggacctgggg cctgtcctgc gactttcact
    27601 gagaaatgga gggcttgctc ctcaacagtg ccaccggaaa ttgtgtgaac acatcctgca
    27661 gccttaaaac gccatcatcc ccggactgaa tttcttcgcc ggtaaaaaca ttcacatagc
    27721 ttctgggtgc ctgcccgggc aaaataacct gtgcctttcc ccaggcatcg cccaggggca
    27781 aatttgtccg catgcccatc aaagagcagg caaagcgtgg cacaatggcc aacactgatt
    27841 ctttggtcgc cgcatcctgc cggaggaaag cgacagcatt ctcctgtttc tctgcttcta
    27901 tgttgagcgc gaggtagtct ccatgtcgga aaagagagtg caactcattc cgcagagaca
    27961 gtgcgcgatg catggtccag agcttgatgc gtccatcagc aagcgtttgg agtacattgg
    28021 tacagaccgc cgctggtcct tctctttccg cttgctcacg cagacgacgg agggcatcca
    28081 cgcgctcttc aaaattcaca ggccgacgat tgtcaggatc caccaggctg aggtcccaca
    28141 gctccgttcc ctgatagaaa tcaggaacgc ccggggaggc gattttcaag acgatctgag
    28201 agagggagtt cagcgcccca aagaactgta cggcaggcat aaaagacttc agcgtatgga
    28261 caaagcgcgt ttcctggttg cggccttgcg gaagcagaat gttctcaatg aatttgtgta
    28321 ctgcgtccag atactcggga tcaggattcg tccaactcag gttgattttt gcttcgctga
    28381 aggccttggt gcagtagtcc ttgatgcgat ttagaaaccg ctggcggtca tcggcagttt
    28441 ccatgcgcca gggccatgct cccacaatgg tctgatagat caaatattct tcgttcggat
    28501 caggtgcagt gcgtccatca ttaatcttct gtttatagac agcattcatg cgcatccaac
    28561 gccgtaccat ggaggcccac tgcgatggca tttccgacag cacattcaag cggttgcgca
    28621 catcttcgct gcgcttcgta tcatgcgtcg aggtggtcag catggagtca ggcgatttct
    28681 gcaggcggtc gaggtttgtg gcgtggaaca gctccgtcgt gatgccgaat gaccggatag
    28741 aactgcctac ctcgtttgaa gagaggaagc gagtgtaaac ataaaaagct gtgtcctcta
    28801 cgcccttggc catgactggt cctgtaagct gctggaattt cagagcaaaa tgcaggcgcg
    28861 cagaatattt ctcgtcatct ttgttgccta gtaacagcgt gttcttcaga aagtcgaagg
    28921 cagactcagc aacatcaggg ttgcggtgtt tggcgcgttc aacggcctgg acgatgaatg
    28981 taatgtcgcg ttctgtgtac tggccgcgat cgtctatata cgtgcggtaa acagggaaac
    29041 aagcaatggt ctcgcgaatg acgcgctcca ggatgctctc tgtaaaatcg cgcgcccggc
    29101 ggtcgcttgc agcaaggtaa ttgagcaggt ttgtcaatac gtagatttcg ctggaaagcg
    29161 aggtctgcat cacctgtagc ttgcagcggt aaatcagctc atccggacgg acctcccgtc
    29221 ccagaatctt tgcatacagc gaggtgaact ttgcttcatt gcgtggctcg atgaatatct
    29281 gattggcaag atgcaggaag tcatatccag ttgttccacg cactggccac tcctgcggaa
    29341 gcgcctcacg cggctcaaga attttttcta cgaccgtata aagaggcccc gcactgcgcg
    29401 tccagtcata gccgcggagc gtttcgcgca atacaacttc aatgccattg gcagtcagtt
    29461 cgcccttcgg ttccggacca aagcattgtc cggcaataca gagcagttgc aggcggatga
    29521 gatactgccg cggattgaac atgccgtcgc aatgatcaat acgcagtccg gtcacttcac
    29581 ctgaggcgat catcctgcgg accaggcgat gcgtagcggc aaaacatgcc ggatcttcct
    29641 ggcgcagtcc tacaagatca ttgatgtcaa aaaaacggcg gtaattaatt tcctccgatg
    29701 atacccgcca ataggccagc ctgtaaggct gcaactccag taacgcatgc agttgatcaa
    29761 agctgcgcgg atcaccttca actccgttga tgcgctgaat cgcacgctcc actatgggtt
    29821 ggatctccgg agactgcaat gcctttatca gccgcggctt caggtcgcgc agctgctgct
    29881 ggcgctgtgc aatcaggccg ggatcagacg aatcgttcgg tggaatgtgc gcaagctcct
    29941 tgagcaggtc acgaaacgat gccggcacgc cttctctttc gtcttcccgc tcgggaaaga
    30001 gtacagggat ggaacgaggc gccatgggga aagagtggtc ataatacttg atcttcaggt
    30061 gcccgtcttg aatggcaacc tgtatcttcc cgctttccag ctcctcccca tactggtttc
    30121 ccagcacagg cagaagaagt tttccgcgca tatcaggttt gatcggctcc cattcgatat
    30181 cgaagaagct ggcaaactct gaggtacgcc cattttccaa aacgtcctgc caccataccg
    30241 attcattgcc aacgcccata tggtttggca cgatgtcaag caacagcccc atctcacgct
    30301 ctcgcagggc ggcagccagc cggtgaaagc cttcttctcc gcccagcacc ggattaatcc
    30361 ggtcgtgccg tgtgacgtcg tacccatgcg tgcttccgct tctggcttcg aatatcggag
    30421 aagaataaag atcgctgatt cccagatcat gaagataggg gagtatcttt tccacatcag
    30481 caaacgtaaa accagagtgc acctgcacac gatatgttga tttgggcacg cgagcgtccc
    30541 ggtgcactac ctggaaaatg cagtctctta cttcggacat atattctgga ttgagtcgca
    30601 ggtactgcca gttggatgcg cgaagaagct gaaccgttta ccagcaaggc ggcccgtgca
    30661 gactttacga aatgcgcgcc gtccgcacac aatagagaag aaatatacaa aaacgaggaa
    30721 aaccgatgcc gctctccgga gaagccattc gactgatgaa ttacattgac gatgtcaccg
    30781 taaccctgcg ccgcctcttg tctctggcgc agacgcttcc tccggaggag cgcaagcgcg
    30841 tcggtgagta cctgaaaaac gcccagccca atatcaatac agtgatccag gcactcgaag
    30901 gaaagtgatt cattctgctg tcgtcatggg cgtggcaaag acctgttttc cacgcctcta
    30961 cgattgaaac gatgcccgtg aagcagacat gggtccagtc acgccagcag atgaaattga
    31021 ggtcatttca cggcgacggc agccgcttcc atctctgccg gaggctgagg aggtatgagt
    31081 gaaatctctt cggaaatgct gatgcccgcg cgccgcaata attgtcccac cacggccttg
    31141 ttcaggcgtg tagcatcgta ttccacgcgg atggtctgct cattctcatt caactggatg
    31201 cggcgaatgc cataaacttc gctcaaattg ctcagagcga acatggcagc ctcagaagga
    31261 tgctgttcat agcgaaagag gacgtctacc gaagtcatga attcatgata tcaagcagag
    31321 ttcatgcagc agatggagcg ccgcggccga tgacaggcag aaagatggaa atgacagtgc
    31381 cgcttctgct tgagcagacg taacaaatca cttacaggca gggacatcac agtccatttg
    31441 acctggggta tttcagcact ttttgcactt ggccaggatt gccttttgca aacgctcacg
    31501 cagttcattg ggaagttcat acaactcgtg gctgcgataa atttcaatcg ttttgcgtgt
    31561 ggtgttcaga gtgaccacac agtgctcgca gccacgcaga tgctcttcga gctcagcccg
    31621 cagcgaggcc gagacacggt cttccatcag gtcatccaac aaggcgataa actctgtgca
    31681 ggtcacttcg caccaccatt cctcttgttc ttgcggaagt agcggctcaa ccgctcccgc
    31741 aactgcaaac gcgcccgcaa caagcgcgac tttacagccg ggacgcttag tcccagtgtt
    31801 tctgcggtct cttctgtcga gagaccttcc acatcacgca atacaaacac cacgcggaag
    31861 cccggcggaa gcccctggat ggtcttgcgc agaatctcag ccagctccga ttggctgtat
    31921 tgctgctctg ggtcggggcc ccagtcggcc agatcgcgtg gcatggagcc ttcctcggtc
    31981 tccacgtcct cgtcaatcga aaccatgcgc ccggtgcgcc gtttgcgaag gcgcattaac
    32041 gcctcattga ccgcaatgcg caccagccac gtataaaatt tcgagttgcc ctggaactgg
    32101 tccagtttct cgtaagcctt caaaaacgag tcctgcacca cgtcttccgc gtcctcgcgg
    32161 ttctgggtaa tgtgctgggc aatccggaag acctggcggt catactgctt caccagcttc
    32221 tcgaatgcct caacgtcccc ttcacgcgcc ctgttgacga gcagaacgtc cgggtggacc
    32281 tcttcagtcc cttgtgtttg gatgtctggc atgacagatg gcttcaggaa cttcgacctt
    32341 tcttgaccgg agcagagcag cagggcagaa aagcagggct gtcaggctcc tcctctatat
    32401 taaatgcgtg cgaacaaaag ggccaaagcg gaaagccttc cccaggcggt tcaacccgtc
    32461 tgctctgaag caggtgcaag aatttcacgg gccccctgtt ccagaaggcg ttcggctgtg
    32521 agatgaccta gctgttcggc cgcctgaccg ctctcgtggc gcaattccac acgtaccagc
    32581 gaagagccat ccggatgcgc aactgctccg ctgacgacga tgccctctgg agtattccgg
    32641 cagtagatgc cgatggggac atggcacccc ccgccgagcg cgcctaatgc agtgcgttct
    32701 accgtaacgg cgaagtgtgt ttcctcatcg tccaggaatg cgatggcggc acgaatccgg
    32761 gcgtcgtctg agcgcgtttc catggccagt gctccctggc cgggtgcggg gcacaccacc
    32821 agcggtgaga accgctcccg gacccattct gttcttccca ggcgttccag gcccgccgtt
    32881 gcaacaacga tggcgtccac ctggccgtcc tcaagtttgc gcaggcgagt atctacattt
    32941 ccgcgaaatt cgaccaactc cacatcccgc cgttgtgcgc gaagctgcgc ctgccgccgc
    33001 agactgctgg ttccgatgcg cgcgccggac ggcagcgcgg caaaagaccc gtatttgagg
    33061 gagacaaaag catcgcgcgg gtcttcgcgt tttgcaatgg cggcaatggc aaaagcggga
    33121 ctgatttcag tcggaaggtc tttcaaactg tggacagcga gatcaatcct gcctccgtac
    33181 aaggcctctt caatctcttt ggtgaacatg cccttcgttc cgactttgtt aaaggcgacg
    33241 ttggaaacgg catcgccggt agtgcgaatg atttcaatgg aaacttcgtg tccggcccgg
    33301 cgcagagcat ccgcaatatg attggcctgc cacagcgcga gctgcgagcc gcgggaaccg
    33361 atgcggatca tgggcgggcc ttccgtccgc ctatcgccac aggcacatcc tggctctcca
    33421 ccagggccgg tccggatggc tgctcctgca cagacgacag accaaatatt tcacgcagca
    33481 cttcaatcac cagagcatcg gattcctgcg cggcggcctt gagcgactgc atcggcaggt
    33541 ggaggaactt gttcatcagc ccccgcgtca gcgattccac cgcggcctgc tgctctggcg
    33601 tgagcgattg caaacgcgcc tgcaccctgc gcagctcggc ctgacggatg tcctcagcag
    33661 tgttctgcag ttcgacgatc ataggcgtca cattcaatgt ctgcgctttg cgctggaagc
    33721 gtaatacctc ttgagcaatg atctcttcag cgcgttgcgc ttcgcgggcc cgatcagcca
    33781 gatgcgaggc agctacggat tgcaaatcgt caatgtcata gagaaagacg ccgtctacgc
    33841 ggttcatctc cgggtccacg tcgcgcggca cggcgatatc aataaagaac atggggcggt
    33901 tgcgacgctt gtgcaggaac tgttggccgt gttcgcgacg gaagatgtgg tgctgtgagc
    33961 cggttgaggt gatgatgatg tctgcctggt ccgccgttgc gtacaagtct tcaaagcgca
    34021 ctgcctgtgc ttcaaactcg cgggcgatgc gtaaggcgcg atcgaaggtg cggtttgcaa
    34081 caaggagtga atgcgcgccc tgctgcataa gatgccgcgc ggcaagttcg ctcattttgc
    34141 ccgcgcccac aagcagaatg cgcttgccac tgagggaacc gaagattttc tttgccaggt
    34201 ccactgcaac cgaggcaatc gaaaccgaag agctgccgat ctgcgtttcg ctgcggactt
    34261 ttttcgccac cgtgaacgta gattgcaaca gcctttcaag atgcgtctgc actgcgccca
    34321 cggcacgcgc gacagtgtag gattccttga cctggcccag gatctggggc tcgcctacta
    34381 ccatcgagtc caggctcgac gcaacgcgga agaggtgccg tacggcttcg ccctcacggt
    34441 attcgtaaac gtgtggacgc aactgcgtcg tgtctactcc gagaaatttc tgcaaaaaaa
    34501 ccagaaggtc gggatgggcc gactcgtggc aggccagaag ctctacgcgg ttgcaggtgg
    34561 aaagcaccat tgcctcgctc acgcctggaa agtggcgcag ggcgagtgtt gcttctccca
    34621 gccgctcagg cgcaatggca agctgctccc gcaactcaac aggagcagtc ttgtggttca
    34681 cacctgtcag cagaatcctc atggcgttgt gtacctgtgg accgagctga aaaggttcgc
    34741 tgcccacacg caaagcatca ccagaaaaac ggcgctcgaa agataggcgg ccctgcggcc
    34801 gcgcagccca gtggaacgct gtacaaagac cattacaatg taaagcaccc acattacgaa
    34861 cgagagcacc actttagggt ccagaaaata cgtcggcccc acgtgctcct gggcgataaa
    34921 cgagccaatc agcagtcctc cggtcatgca atagaatcca agcaccagtg tcttctgcgc
    34981 aatctggtcc atcgtgtcca gcggaggcag ccagttcagg aagccgggag tgcgcttgtt
    35041 cttaagacgc cgctcctgca cgagataaag aagactggcc agcaggctga agatgagggc
    35101 ggcataggcc gccagcagca atgcgatgtg aatgtatatc caaccactac ggacaagcgg
    35161 tgaagaaaaa gtgtatctgt ccggcccgaa cgacggcatc agaagcagaa aaaatgccag
    35221 cggcagcgca aacacgctga aagagatcgc ccggtaccgc agccagatga gcagaaacgc
    35281 ggccacaatc agaagcgaca gcgtagactg tacctcgtgc atgccgaccg gcatccagcg
    35341 atgcgcggca accagaattt ccaccatcgc caccacatgg tagaaccatc cggcaatcgc
    35401 aaggaccaga gatgctttct gccatcgtgg acggtcgcca agcacagcgg gcaacgcaga
    35461 cacgcttgcc agtccgtaca gcgcagccgc cactctgagc caaagcaaaa acatgggtaa
    35521 tacttcagta taaagcggta aacgccagag cgcagacctc ggctgcgggc atcatcgccg
    35581 gcgcctgggg attcagtacc accccacatc tcgaagagat atgcgaatat gcgatattct
    35641 ggcctttccg tgcctgaaac atccgtccaa tccatagaaa cagcccagac tccgctcatg
    35701 cgccagtacg cggcagccaa gcgcgagcac cctgacgctc tgctcttctt tcgcctgggc
    35761 gatttctatg aactgttttt tgaagacgcg tacatcgcct cgcgcgaatt gcagattacc
    35821 cttaccgccc gcgaccggga aaagtctcag cccatgtgcg gcgttcccta tcacgctgcg
    35881 gaggggtata tcgcgcggct gctgcgcaaa gggtacagag ttgccatctg cgagcagatg
    35941 gaagacccga agctggcgaa aaagatcgtc cggcgcgaag tgacacgtgt gctcactccg
    36001 ggaacggcga tagaccccgc gttaggctcc gaacagaaca actttcttgc cgctgtgcat
    36061 gtcgaagaca agctggccgc gcttgccctg cttgaccttt ccaccggaga tttccggacc
    36121 actgaattca ccgggccgca ggccctcgcg caatgcctgg atgaactcga aaaggccgcg
    36181 ccaagcgagg tacttctcgc cgcatcggcc agctttgaca atacgtccgc actggaccgc
    36241 atcaggacca aaacttccat cgaggactgg gtgtttacca gcgactatgc tgttcccctg
    36301 ctggagcgcc agcttggaac agcttcgctt gacggtttcg gccttgcgca ccacccggcg
    36361 gcggccattg ctgcgggcgc ggtcctgcat tatgtgcgtc aaacacagcg gacagacatc
    36421 agtcacattg acagcatccg cttttacgag cgggccgaac atctgcagct cgaccaggtc
    36481 accgtccgca accttgagct ggtcgagccg attttcagcg aagtggggcg ggaaaccacg
    36541 ctctttcaga cactggatgc ctgccagacc ccgatgggca agcgcctgct gcgagcttca
    36601 attttacggc cactgttgaa tgagcacagc atcgagcagc gctacgaagc ggtggacctg
    36661 ctcgtccgcg acctcgtgct gcgcgaagag ctccgccgct gtctgggcgg cattcttgac
    36721 ctcgaacgcc ttctggcccg tatttctctg gatagcgccg ggccacgcga tgttctggcc
    36781 ctggctgctt cgcttcagca ccttccgcct ctgcgccaga ttctggaaaa gcccgaagcg
    36841 ccgctctgga agcagattac agagcgcctc gacccgcttg aagatgtctg cggcacaatc
    36901 cggcagacca tccggcccga ccctccgttg acgctggccg atggcgacgt cattgcccag
    36961 ggtgtagacg cggagcttga tgagcttcgc gcattgagcc agagcgggcg ccagtccatc
    37021 gccgccattg aagaacgcga gcggacacgg accggaattt cgtcgcttaa ggtgcggttt
    37081 aactccgttt ttgggtatta catcgaaatc acaaaggcaa acctcgccaa tgtgccgccc
    37141 gattacgagc gcaaacagac gctggctaat gcagagcgct tcaccactcc ggaactcaag
    37201 gactacgaaa cccgcgttct taccgcgcac gaacggtcta tcgaaataga aaagcgcatc
    37261 ttcgccggac tgcgtcgcag tgtcctcgaa catgccagcc gcatccgcaa gtcaagttct
    37321 gcggtggccg aagccgacat gctggcaaac tttgcgcatc tggcggccct gcgtaattac
    37381 agccgccctc agcttgtttc tgaatctgtt ctggagatcc ttgcaggacg gcaccctgtc
    37441 gtcgaacgcc tcatggaagt gtccggggaa ggcagttttg ttcccaacgg cctttatctc
    37501 gacgtggaag gccccgctat tttgcttctg accgggccta acatgggcgg taaatccact
    37561 tatcttcgtc aggcggcgct tctgacgatt atggcgcaaa tgggctgcta tgttcctgcc
    37621 gagcgcatga gatatggcct cgtagaccgc atctacacgc gcattggcgc cagcgacaac
    37681 gtgtcccgcg gccgctcgac ctttatggtc gagatgaccg agacagccac aatcctcaat
    37741 accgccaccc ttcgctcgct catccttctc gacgaaatgg gccgtgggac agcgaccttc
    37801 gatgggcttt ccctggcatg ggccacgctc gagtacctgc acgcgaaaat cggcgcgcgc
    37861 acgctctttg ccacgcatta tcacgagctt acgatcctgg ccgagcagct tccgcgcctc
    37921 aaaaacctgc gcgtcgcggt caaagagacc ccgcagggca tcgtctttct gcattcgatt
    37981 gagcccggcc ccgcaaacaa gagctatggc atcgaagtag cccgcctggc cggacttccg
    38041 ccgcaggtca tccaacgcgc ccgccaggtg ctcaaacagc acgagcgctc tgaacggcat
    38101 aatgttgccg cagaaaccga ggccccattg caactgacga tgtttactcc gctctcgcag
    38161 cgcatcatag accgcctgac tgagacggac atcaacgcgc tcacaccgct tcaggcgctg
    38221 aatctgctcg aacagttaca aaacgagatc aaaggcggaa aagcatgagc gaaacgcttc
    38281 gccagcaggt aggaaagctc ttgattgttg gactggaagg taccgcactc accgccgtgg
    38341 agcgcgcctg gctcaggctc attcgccccg gcggcatcat tctctttcgc agaaacattg
    38401 aggaagctgc gcaaacagcc gcattgctgc gcgaaaccac agagatcatc ggcgcaccca
    38461 tcttccgctg cgttgacctt gaaggtggac tggtagaccg cctgcgcgac ctgattgcgc
    38521 ccatgccttc agccgcccag gtcttcgcca caggcaagcc tgctctctac aggcagcacg
    38581 gccggcttat tgccagagaa gcaaaattgc tgggctttaa cacggtcttt gctccggtac
    38641 ttgacctcgc ccttcccgaa tcgagcacgg tcatgcgcac gcgcgtggtt tccgctaacc
    38701 cgcaggatgt aattacatat gcgtcaggat ttcttgatgg gctggaagca gaaagcgtgt
    38761 tggggtgcgg gaaacacttc cctggtctcg gaggcggtaa tctcgactcg catcactcca
    38821 cgccactcat cgagcgccca tgggagacca tgtggaatga agacatagcg ccatttcgcg
    38881 cgctcgcgcc acggctctct atgatcatgg cgtcacatgc catttaccca aaggcatccc
    38941 acgagcgaag gcccgcatct atatcggcgt tttggatttc agaaattctg cgaagaaaaa
    39001 tcaaatatac gggcctggtt gtttcagacg atatggaaat gggtggaatt ttgacccaac
    39061 agcgcattga agatgcggtg attgcagcag tttccgcagg cacagacgtg gtcgagatat
    39121 gtaaagaccc cgcgcttgtg ttacattctt atgaggcgct gttaacagaa gcagagaaat
    39181 ctacagcatt tcgccagaaa ataaccagag catttcgccg catttgtaag cttgctcccc
    39241 cgaaacagtt atttgcgctc ccagctcatt ctcgaattct gggccttcgt gaaaaagtca
    39301 aagttttttc ggacataaat ataaattctt tttcatcttc tgttcttcaa tcgggaggta
    39361 atgtgtatga gaataagaat aagtaataag ttggtacctt actttcggat agtccccaga
    39421 caaagacttt tgtcacattt cgagttacac aattttatct ggctaaaaga ccaggatttg
    39481 tagtatatac ttcaggttaa agacgagctg tcaagtggct gtaagatgcc tccctttcat
    39541 cgcaagctgc tgatcaagta aatataaatt tgcagtagta gcattcacag aacgctcccc
    39601 gggattgacg tgacgcaaat ggcgacttcg gaatatattc gaaaaaccac tggggcaggg
    39661 aaaaagtctg ttcaggctgt ccccagcctg attgcgagtg atgaccggct gtcgggacct
    39721 gcgctggctt caaagctgat gctggcaatg agcgacgtcc ttgtgatttc actggcgttc
    39781 gtattcgtgc tcatcctcaa ggtcaggttt tttgccgggt atggcgcgtt tcgctccctg
    39841 gagatagtaa aaacaccgat ttaccttgcg ttcttcggat ggtttatctt gtccttgctt
    39901 ttcgtctgcc gcagatatgg cctctataac ctcacccggc cattcagtgg agcacatgag
    39961 ttaagactcg tagtacaggc gacgcttact gccggcctcc tactctgcgg cggcctgtat
    40021 atggcgcact gggacgtggc ctcgcgaacg gtcgtggtac tgctgatctg caccagcgcc
    40081 atctctttga gtatctggag atcaggcctg cgggttgcgc gctatcgcaa ctttgaaaaa
    40141 ggcgtaggca ctcgaaatgt tgtagttctg ggcacgaact atttgagtta tgcgctgggc
    40201 gaacacattg ccagcaacta caggctggga tacaaattta tcgggcatat cgcgccttct
    40261 tccagcccag atgaacatac agtggtgagc aatgttctcg ggcacatgga cagactacat
    40321 cagttaacac gtctgcattt cattgacgaa gttgtcatag cccaggcact tccgaatgag
    40381 atcgtcatca gcttgctcga agaagcacgc gagctgggga tagacattcg cgccatatcc
    40441 ggactctaca gtgatctggc gacgaactcc tccattgaat atctcggcgt ctatcctgta
    40501 acggcccttc atcgcagccg ctctaaggtc atttcctcgg tcttcaaacg ggtctttgac
    40561 attatttttt cgcttgccgc actggccatt gcgcttcctc tgatgctggt tgtggcgctg
    40621 gcggtccggc tcgatagccc gggacccatc ttctatatct ccgagcgcgt gggaaagcgc
    40681 ggacgcattt tcccctgctt taaattccgc actatggtca aagacgcgga gaaaaagaag
    40741 aaagagcttg cggcgcttaa cgagcgcgat ggtattttat ttaagatttc caatgacccg
    40801 cgcatcaccc gcgtaggacg cttcctgcgc aaatacagcc tcgatgagct gcctcagctc
    40861 ttcaatgtcc tgcgtggaga aatgagcatt gtaggacccc gtccacccat tgccagcgaa
    40921 gtggcccagt acgaactgga acacttccgc cgcctggagg tgactccagg gctgaccggg
    40981 ctgtggcagg tacaggcacg tcaggattca tcgttcgata aatacatcga gctggatacg
    41041 gcctacgtgg aaaactggag cttctggctg gatgtgaaaa tcctgctccg gactgccctg
    41101 gtggtcgtcc gcggaaccgg aacataaacg gtcctcttcg actgccccga cgcgagtgcc
    41161 ggggcgaaaa tcctctaaac cgccgtggct gtcgccagca ccccatcctt taagaacatc
    41221 tgcttgattc ctcgtagtgc ctggcgcgtc cgctcttcat tttcaataag cgagaaccgc
    41281 acatggccat cgccaaattc tccaaagcca atgcctgggc ttaccgcaac tttcgcatct
    41341 ttcaggagct tcgtggaaaa ctcaagagaa ccgagtgcgc gatacgtttc agggaccggg
    41401 gcccagacga acatggttgc cttcggcatg ggcacctgcc atccgagacg gttcaggcca
    41461 ttcaccagca catcgcggcg tttgcggtag ttctcgcaaa tctcatggac acaatcctgc
    41521 gggccttcca gcgcaagaat cgaagccacc tggattggcg caaatgttcc gtaatcaaag
    41581 tagctcttga tgcggcccag cgcgcctacc agcgtcttgt tcccaaccat aaagccaacg
    41641 cgccatccgg gcatgttgta gctcttcgat aaggtaaaga actccaccgc gatgtccttt
    41701 gcgcccggga cctgcagaat agacggggcc ttatatccat caaaacagat gtctgcatac
    41761 gcgagatcat gcaccaggta gatgttccgc tccctgcaca atgccaccac acgttcaaag
    41821 aaatggagat cggcacactg cgtcgtgggg ttcgccggaa aattcagaat catcatcttc
    41881 gggcgcggat acatcaaagg aatcgtccgc tccaactgcg ccagaaattc ttcagtatca
    41941 tgaatggcca cgctgtggat attcgccccg gcaatcaccg gcccgtaaat gtggatggga
    42001 tagctggggt tgggtactac gactacatcg ccctggtcga gcgtggccag gcacaagtgg
    42061 gcaatccctt ctttggatcc gatggtaaca atggcctctg tgtccggatc gagcgccaca
    42121 tcataccggt cctgatacca gttgcaaatc gctttgcgaa ggcgcggaat gccgcgcgaa
    42181 agcgaataac ggtgcgtctc cggtttacgg gccgcctcta tcatcttgtc cacaatatgc
    42241 tggggcgtcg cgccatcggg attacccatc ccgaaatcaa taatgtcttc cccgcgtttg
    42301 cgggcggcgt tcttcagttc cgcggtgatg ttgaaaacgt aggccggcag gcgtttgatt
    42361 cttggaaact cttccacgcg tcagtcctct ggatgaactg acttcactat agaggatgcc
    42421 tggacaattt tcactcatcc gccaagtgct gcctgcaggc ggtcactata acttcggctc
    42481 acgcgcaacg gagtgccgtc cttcaggcgc agcaggcaat cgcgattggc cagcgcctgc
    42541 atttccgcaa tacggtccag ccggacaatc gtcgaacggt gaatccgaat aaatgccgcc
    42601 gggtcgagcc ggcgttccag aatatgcagc ggttcacgca acagatgcgt ctttccgttc
    42661 acatggagcg cagcatagtc gccctgtgcc tcaatccatt ccacttcatt ggctgttaca
    42721 aagaaaatgc gccgcccatt gcgaatggca aagcgctgcg catttcctgg tgcccgcctt
    42781 gcatggaggt ctgagagcaa agactccagc cgctcgctca acgtgttgtc gcgctccatc
    42841 tgcaacatgc gctcgacgcg ctccacggct tcgttaaagc ggacatcgtc aatcggcttc
    42901 aacaaatagt ccgtggcatg cacctgaaag gcccgcagcg cgtattgatc gaaagcagta
    42961 aggaagacaa ctgcgggacg ttcctccaca ggcaggtgct ccagcatttc aaagccgtcc
    43021 atctccggca tctgcacgtc gagaaaaatc aggtcaggct tctgctctgc gatgacctgc
    43081 aatgcatctt cgccattagc gcattcggca atcacctgaa actgagaata gggttgcagg
    43141 cgcgaaacta ttcccctccg cgccagcggc tcatcgtcca ctacaaccac ccggtatgtc
    43201 atggcagtcc actccctgtt ggtttttcgc tttctacttc ggcaggagac gcatacaaat
    43261 ggaacgggat cctgattgtg accttgcaaa cgtcgccctc gcaaaaatca attgtgaagc
    43321 gatgcgcgtc tccatatagc tgcttcaggc gggtctgaat gttcgagagg ccgatgccat
    43381 ttctgaactt tgtgccattc agcggcatca cgtcatttgt tatctgaatc accaggtctt
    43441 cttcgtcccg gcgtgtggac atgaacagcc ttccaccctc gcgccgttta cctatcccat
    43501 gccgcaccgc attttccacc agcggctgca ataacaggcg cgggaccgcc gcatccagaa
    43561 cagtggggtc tgtctcgtcc acgatgatga gcctgtcgca tagacgcgtc ttttcaatct
    43621 ccagataaag attggtaatg tccaactcat cacgcaatgg gacttcgtag gcgccatcat
    43681 catcgagtgt agcgcgcagg aaatccgcaa gccgtgcaat catgcggttc gctgttgccg
    43741 tgtcatgctc
//
LOCUS       DASRBV010000189         6157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_468528, whole genome shotgun sequence.
ACCESSION   DASRBV010000189 DASRBV010000000
VERSION     DASRBV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468528"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1500)
                     /gene="atpD"
                     /locus_tag="VFA02_07395"
     CDS             complement(58..1500)
                     /gene="atpD"
                     /locus_tag="VFA02_07395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZU09712.1"
                     /translation="MAENIGKVIKIAGPAVDVQFEESKMPPIFQALRVVSEGFDVPQP
                     IDVILEVQQHLGEGRVRCVAMEPTEGMVRGMKAIDLGGPITVPVGRETLGRVLNVIGK
                     PVDELGPVNAKEHLPIHRPAPAFEEQSTREEMFETGVKVIDLIQPFLKGGKIGLFGGA
                     GVGKTVIIQELINNVALKHGGFSVFAGVGERTREGNDLWIEFQEAGVIDLKDLSKSKA
                     ALIYGQMTEPPGARLRVALTGLTVAEYFRDNEGADTLLFIDNIFRFTQAGSEVSTLLG
                     RMPSAVGYQPNLATEMGELQERITSTKKGSVTSVQAVYVPADDLTDPAPATTFAHLDA
                     TTVLSRPLSELGIYPAVDPLASTSRILTPRVVGQEHYDVAQGVKRILQRYKDLQDIIA
                     ILGIDELSEEDKLTVARARKIQRFLSQPFHVAEQFTGIPGRYVKIEDTVRSFKEVLEG
                     KHDDIPEQAFYLKGSIEDVLEAAEKMKANA"
     gene            complement(1517..2584)
                     /locus_tag="VFA02_07400"
     CDS             complement(1517..2584)
                     /locus_tag="VFA02_07400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HZU09713.1"
                     /translation="MANVLDLRRRIRSVKNTRQITKAMKMVAAARLRRAQEKALSSRP
                     YAMMMTSVLQSLRRRVDAVDPETGQAHPLLVVRPEKNILLIVVSGEGGFAGAFNANIV
                     KAANIFIAERKDAQIDIEAIGRKGRDVLRRRYPLAQWADAEKQEERRRIAPVELLEDH
                     HHGILHKLTFEQVSGLAHEIISLYAHEKIDAAYVVYNEFKSVIQQRVVVERVLPVLEI
                     GKREVALAQEMTQEERERAGEAAISSGVTLSETEAEADAKQAEEEARRFGTAEVDYIY
                     EQNPGELFNALLPRYVATQLYHAMLESTAAEFAARMTAMDSATNNASELIDSLTLAMN
                     RVRQAAITKELIEIVSGAAAL"
     gene            complement(2723..4270)
                     /gene="atpA"
                     /locus_tag="VFA02_07405"
     CDS             complement(2723..4270)
                     /gene="atpA"
                     /locus_tag="VFA02_07405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZU09714.1"
                     /translation="MALIKADEITQLLKEQIQNYDAKVRVDEVGTVISLGDGIARIHG
                     LDKVMYGEMIEFPHGVYGLAMNLEEEQVGAVLMGDYTEIREGDQVKRTGKILSVPVGE
                     AMVGRVVNSLGQPIDDKGPIQTNESLPVERIAPGVIDRQSVREPMMTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAIALDTIINNAKNDLICIYCAIGQKRSSVAQVVQTLTEYGA
                     MDYTIIVAATASEPAPMLYLAPYAACAMGEYFRDRGKHALVIYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSKEKGGGSLTALPVIETQAGDVSAYIPTN
                     VISITDGQIFLETDLFNSGVRPAVNVGLSVSRVGFSAAMKAIKQVGSTLKLDLAQYRE
                     LAAFSQFGSDLDKVTQQQLARGQRLTELLKQPQFHPLTAEHQVAILFAGTQGMLDDVK
                     VEEIRAFEEGFHKYLDSAQTGLLKEISEKKALDDDLRNKLKAAINEYKQNFFAEHESA
                     QVVGAGR"
     gene            complement(4348..4884)
                     /gene="atpH"
                     /locus_tag="VFA02_07410"
     CDS             complement(4348..4884)
                     /gene="atpH"
                     /locus_tag="VFA02_07410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZU09715.1"
                     /translation="MAAVAAPYARAFADVVLEAHLNANDVQKQLDNFVAVWWESDDLR
                     EVFLDPTFPADQKVAILDKLNERLEMSQQVRNFLAVLIQHDRMDILGEILDEYRREMD
                     RRLGISEVHVTTARPLEADERRGIEQKVASLTGTQVRTQYFEDKSLLGGVVVRVGSTV
                     YDGSVRGRIERLRKQLVG"
     gene            complement(4884..5600)
                     /locus_tag="VFA02_07415"
     CDS             complement(4884..5600)
                     /locus_tag="VFA02_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HZU09716.1"
                     /translation="MKRSFRLKLAIFVAAVLLVSAPATRAIAQEKPAQPQGGASSYEK
                     MDAPETNSEEEGYRHSATVQAIAKYLHTDTETATQIFEDFNSAVLILAIAGFLIKVLP
                     KTFRKRSETISKELAEARSATEDANQRLSTVEARLAKLDSEIDAIRRQTEQEMVEDEK
                     RIKAQIEEERKRIIESAEQEIEAFSAAAQRDLKKFAADLAVERALQRIHLGADADRIL
                     VQNFTAELANTIQNGKGGQN"
     gene            complement(5597..6064)
                     /locus_tag="VFA02_07420"
     CDS             complement(5597..6064)
                     /locus_tag="VFA02_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09717.1"
                     /translation="MDEILRQLGDLGIGSVPTMIIFIVLVLAYRFLVYKPLTRTLAER
                     RERTKGAIERAQAAIAAADARTQEYEAKLRAARADIFRHREQRIQQWNTERDHALASA
                     RLTAQERVRAAQASIAQQAAEARKQIEGSTEQLASQILKAILPAGVAPVESVR"
BASE COUNT         1180 a         1973 c         1636 g         1368 t
ORIGIN      
        1 tccaccagaa cgcggtccgg ggtcacaagc cggatgtgga gttggttcgc catggtctta
       61 tgcgtttgcc ttcatctttt ctgcggcttc gagtacgtct tcaatcgagc ctttcagata
      121 gaacgcctgc tccggaatat cgtcgtgctt gccttcgagt acttctttaa agctgcgcac
      181 cgtgtcttcg atcttgacgt agcggcccgg gatgcccgta aactgctcgg ccacatggaa
      241 cggctgcgac aggaagcgct ggatcttgcg cgcgcgcgcg acggtgagct tgtcctcttc
      301 agaaagttcg tcaatgccca gaatagcaat gatgtcctgc aaatctttat agcgttgcag
      361 gatgcgtttt accccctgcg ccacgtcgta gtgctcctgc ccgaccacgc gcggcgtaag
      421 gatgcgcgag gtcgaggcca gcggatcaac ggcggggtag atgcccagtt ccgacagcgg
      481 gcgcgagagc accgtggttg catcaaggtg agcaaaggtc gtggccgggg ccgggtccgt
      541 caggtcgtct gccggaacat acaccgcctg caccgaagtg accgagcctt tcttggtcga
      601 ggtgatgcgc tcctgcaatt cgcccatctc tgtagcgagg ttgggctggt atccgacggc
      661 ggaaggcatg cgacccagca gtgttgacac ctcagagcct gcctgcgtaa agcggaagat
      721 attgtcaatg aagagcagcg tgtccgcccc ttcgttgtcg cgaaaatatt cagcaaccgt
      781 cagcccggtg agcgccacac gcagacgtgc gcccggcggc tcggtcattt ggccgtagat
      841 caaagcggcc ttcgacttcg aaaggtcttt caggtcaatg acgcccgctt cctgaaactc
      901 aatccagagg tcattgccct cgcgggtacg ctcgcccact ccggcaaata ctgagaagcc
      961 gccgtgcttg agtgcaacgt tgttgatcag ctcctgaata atgaccgtct tgcccacgcc
     1021 cgcgccgcca aagaggccaa tcttgccgcc cttcagaaat ggctggatga ggtcaatgac
     1081 cttcacgccc gtctcaaaca tctcttcgcg cgtagattgc tcttcaaacg cgggagcggg
     1141 gcggtggatc ggcagatgct ccttagcgtt gaccggaccc agttcatcca ccggcttgcc
     1201 gatgacgttg agtacgcggc ccagcgtctc gcgccccacg ggaacggtga tcggaccgcc
     1261 cagatcaatg gccttcatgc cgcgcaccat gccttcggtc ggctccatgg cgacacaccg
     1321 cacgcggcct tcgcccaggt gctgctgcac ttccagaatg acgtcaatgg gctgaggaac
     1381 gtcgaagcct tcacttacta cgcgcagcgc ctgaaagatg ggcggcatct ttgattcttc
     1441 aaactgtacg tccactgctg gtccggcgat tttaatcact ttgccaatgt tttctgccat
     1501 aagaatttct ttctctctac agtgcggctg cgccgctgac aatctcaatc agttccttgg
     1561 taatggccgc ctgacgcacg cggttcatgg ccagcgtcag cgaatcaatc aattcagaag
     1621 cattgttcgt tgccgaatcc atcgccgtca tacgcgcggc aaactccgct gccgtggatt
     1681 ccagcatcgc atgataaagc tgcgtggcaa cataccgtgg cagcagggca ttgaacaact
     1741 cgcccggatt ctgctcgtag atgtagtcca cttcagccgt gccaaaacgc cgcgcctctt
     1801 cttcagcctg cttagcgtct gcctctgctt ccgtctcgct caatgtcacg ccggacgaga
     1861 tggccgcctc accggcgcgt tcgcgctctt cctgcgtcat ctcctgcgca agagcgactt
     1921 cccgctttcc gatttcgagg accggcagca cgcgctcgac gaccacgcgc tgttgaatga
     1981 ccgacttgaa ctcgttatag acgacgtatg cggcgtcaat cttctcgtgc gcgtaaagcg
     2041 aaatgatctc atgcgccagt ccgctcacct gctcaaacgt gagcttatgc aggatgccat
     2101 gatgatggtc ttccagcagc tcgactggag cgatgcgacg gcgctcttcc tgcttctcag
     2161 cgtccgccca ctgggccaga ggataccgcc gacgcagcac atcgcgtccc ttgcgcccaa
     2221 tggcttctat gtcaatctgc gcatctttgc gctccgcgat gaagatgttt gccgctttga
     2281 cgatgttcgc gttgaacgca cccgcaaagc cgccctcgcc ggaaacgacg atgagcagaa
     2341 tatttttttc cggccggacc accagcagcg gatgcgcctg cccggtttcc ggatccaccg
     2401 catccacgcg ccgccgaagt gactggagca ccgaggtcat catcatcgca tacggacgcg
     2461 acgacaaggc cttctcctga gcgcgccgta gccgggccgc cgccaccatc ttcatggctt
     2521 tggtaatctg gcgcgtgttt ttcacactgc gtatgcgccg ccgtaaatcg agaacgtttg
     2581 ccatttatct atctgtctgt tgtcatcctg agtgaagcga aggaacctta gcgatgtggg
     2641 ttctacgtaa gtcgctatga ttcttcctcc cttcggtcgt cagaatgaca atcagttatt
     2701 ccaatccagg tcaggtttca aactaccgtc cggccccaac cacctgcgcc gactcgtgct
     2761 cggcgaagaa gttctgctta tactcattga ttgcggcctt caacttgttc cgcagatcat
     2821 catcgagcgc cttcttctcg ctgatctcct tgagcagccc tgtctgtgcc gagtccagat
     2881 acttatggaa gccctcttca aaggcgcgga tttcctccac ctttacgtca tcgagcatcc
     2941 cctgtgtgcc ggcgaacaga atggccacct ggtgctcggc cgtcagagga tggaattgcg
     3001 gctgcttcag caactccgtc aatcgctgtc cgcgtgcgag ctgctgctgt gttaccttgt
     3061 cgaggtcgct gccgaactgt gagaatgccg ccagctcacg gtactgcgcc agatcgagct
     3121 tcagcgtgga gccgacctgc ttgatggcct tcattgccgc cgaaaagccc acgcgcgaaa
     3181 ctgacaggcc aacgtttact gccgggcgca cgcccgagtt gaacaggtcc gtctcaagga
     3241 aaatctgccc gtcagtaatc gaaatcacgt tcgtcgggat atacgccgaa acgtcgccag
     3301 cctgcgtctc aatcaccgga agcgccgtca gcgagccgcc gcctttttcc ttgctcagct
     3361 tggctgcgcg ctccagcaga cgcgagtgca gataaaacac atcgcccgga taagcctcgc
     3421 ggcccggcgg acgccgcaac agcagtgaaa tttcgcggta cgctgctgcg tgcttcgaga
     3481 gatcgtcgta gatcaccagc gcgtgcttgc cccggtcgcg gaagtactca cccatcgcgc
     3541 acgcggcata cggagcaaga tagagcatcg gggccggctc tgacgccgtg gctgcaacga
     3601 tgatggtgta gtccatcgcg ccatattccg tcagcgtctg gaccacctgc gccaccgaag
     3661 agcgcttctg cccgatcgcg cagtagatgc agatcaggtc attcttcgcg ttgttgatga
     3721 tggtgtcgag cgcaatcgcg gtcttgcccg tctggcggtc gccgataatc agctcgcgct
     3781 gcccgcggcc aatcggaatc atgctgtcaa tggccttgat tccggtcatc atcggctcgc
     3841 gcaccgactg gcggtcaatc acgcccggag caatccgctc gacgggcagg ctttcgttcg
     3901 tttggatcgg gcctttgtcg tcaatcggct gtcccagcga gttgaccaca cggccaacca
     3961 tggcctcgcc cacaggcaca ctcagaatct tgcccgtgcg cttcacctgg tcgccctcgc
     4021 ggatctcggt gtagtcgccc atcagcacgg cgcctacctg ctcttcttcg aggttcatgg
     4081 cgagtccata gacaccatgc ggaaactcga tcatctcgcc gtacatgacc ttgtccagac
     4141 catgaatacg ggcaatacca tcgcccagcg agatgaccgt gcctacctcg tccacgcgga
     4201 ccttcgcgtc gtaattctgg atctgctcct tcaggagctg tgtaatctcg tctgctttaa
     4261 tcagcgccat tgctttttgc cttaactcac tctcaaaact gccttagaac ctgtcatcct
     4321 gggccggggt gcctcatctg aaggcaatca ccccaccaac tgctttctca atctctcaat
     4381 ccggccccgc accgaaccgt catacactgt gctgccaacg cgcaccacca caccgcccag
     4441 cagcgacttg tcctcaaaat actgtgtccg cacctgggtc ccggtcagcg acgcaacctt
     4501 ctgctcaatt ccgcgccgct cgtctgcctc caaaggacgc gccgtcgtca catgcacttc
     4561 tgaaatcccc agccggcggt ccatttctct gcgatattca tccaggatct cgcccagaat
     4621 gtccatgcgg tcgtgctgga tcagcaccgc aagaaagttc cgcacctgct gcgacatctc
     4681 cagccgttca ttcagcttgt ccagaatggc aactttctgg tcagccggga acgtgggatc
     4741 cagaaagact tcgcgcagat catcgctctc ccaccagacg gccacaaagt tgtccagttg
     4801 cttctgaaca tcattcgcat tcagatgcgc ttccagcacc acgtccgcaa acgcccttgc
     4861 ataaggagca gccacagcag ccatcagttc tgccctcctt tgccgttctg gatagtgttc
     4921 gcaagctcag cggtaaagtt ctgcaccaga atgcggtctg catcggcccc cagatggata
     4981 cgctgcaagg cccgctccac ggccagatca gccgcaaact tcttcaggtc gcgctgtgct
     5041 gctgcgctga aggcctcaat ctcctgctca gcggactcaa tgatacgctt gcgctcttcc
     5101 tcaatctggg ccttgatgcg cttttcgtct tccaccatct cctgttcagt ctgacggcgg
     5161 attgcgtcaa tttccgaatc gagctttgcc agcctagcct cgaccgtgct caagcgctga
     5221 tttgcgtctt ccgtggcgga acgggcctcg gccaactctt ttgaaattgt ctcggaacgt
     5281 ttgcggaaag tctttggcaa taccttgatc agaaaacccg caatggccag aatcaggacc
     5341 gccgagttga agtcctcgaa gatctgcgtg gcggtctcgg tatccgtatg caggtatttc
     5401 gcaatggcct ggacggttgc cgagtgcctg tagccctctt cctcgctgtt cgtctccggc
     5461 gcgtccatct tctcgtagct ggaagcgccg ccttgcggct gcgcaggttt ttcctgagca
     5521 atggcccgcg tcgccggagc ggaaaccaaa agaaccgctg ccacaaaaat cgccaatttc
     5581 agccggaaac tacgcttcaa cggacactct ccacgggggc cacacctgcg ggaagaatgg
     5641 ccttaaggat ttgggaggcc aactgctccg ttgagccttc gatctgctta cgtgcctcag
     5701 cagcctgctg tgcaatggat gcctgcgcgg cccttacccg ctcctgagca gtcagacgcg
     5761 ccgaagccag cgcatggtcg cgttccgtgt tccattgctg gatgcgctgc tcgcggtggc
     5821 ggaagatgtc tgcccgcgcc gccctcagtt tcgcctcata ttcctgtgtc ctggcatcag
     5881 cagcggcaat ggcggcctgc gcccgctcaa tggcgccttt ggtgcgctcc cggcgctccg
     5941 ccagagtgcg cgtcagcggc ttatatacca gaaaacggta agccagcacc aaaacaatga
     6001 aaatgatcat ggtcggcaca gagccgatgc ccagatcgcc gagttgtcgc agtatctcgt
     6061 ccatgcccta gctgcgtttc acctcgtcca caccacctcc agcgccttgc acttcggctt
     6121 cggcgggctt caaagcagcc agttcagcct gggattc
//
LOCUS       DASRBV010000190         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_64151,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000190 DASRBV010000000
VERSION     DASRBV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64151"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1380
                     /locus_tag="VFA02_07425"
     CDS             193..1380
                     /locus_tag="VFA02_07425"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU09718.1"
                     /translation="MQSGNHLLKSVFVAVVIAAAACFLSVSASAQTSFGRISGTVSAP
                     DGSVVPGAQVIVKNTETQLTRQTTTDQQGFYVFTELPIGTYSVEVNQTGFSKSTRSGL
                     VLVADGRLTADFTLVVGSTSQTVEVQGTNTETLNTTSGELSRVIDTKQVANLPLNGRN
                     YIQLMTLVPGAVVTNPDQFSVTTSLAANNQTINGNRGDSNNLTVDGAYNMVAGSNTSL
                     MNNVSAEFINEVKLQTSNFSAEYGRMSGPAFNIVTKNGTNDFHGSLFEYFRNDVLDAT
                     NYFAPSKTALRFNDFGYSLGGPIIHNKLFFFVGEEWKRLRQNQTTQRQTIPDSAFLSG
                     DFSALLPKTQLYYPGTKTPIPNNNVSNLITPDGKAIANVYALMAKTGAKFVDAPVANN
                     LTR"
     gene            1593..3494
                     /locus_tag="VFA02_07430"
     CDS             1593..3494
                     /locus_tag="VFA02_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09719.1"
                     /translation="MNEARVNGSWVSQHIIPYGTAWQRGTYGFQYPLLYGGADYPNGI
                     PNVSVTGFANFQGPYFALKSPSTDIQIADTISWTHGQHVLRAGAVLIRDRVDQNGRSL
                     YDGSISFNASNNPESTGYAMADALLGNFSSYTEASADPVGFFRFWQPEAFVQDTWHVT
                     PKLSLELGLRWQYMQAIYTQANNTANFDPSRYDPNQAVQITHDSKGLIVPNSGNPYNG
                     LVRAGSGIPKDQTSRVPGANGPLYSVIPAGAPRGLYPGSNNFAPRLGFAYAVDDKTTL
                     RGGFGLFYNRAEGNIVFSQVNIPPFLQISTYSNSNLSNITGGSTSQAPLGGISAIDPH
                     LKSQTVAQYSLNLQHEMPWGLLAELSYVGTEGRHMLRQPNINYPDLSQVAANPKVSTN
                     AFVPYKGFSSITQIRSDSTANYNALQAYVSKRMGALQLTAGYTWSKALADSSSESDNS
                     ENWTVRHYNYGPTSFDRRNAFFSTFVWQMPKLAGHNAWVRSTAGGWQLSGVARLQSGQ
                     YYTVTGTTATGTRRADYLGGSLHVNHPGIQKWFNTSAFAVAPNGRFGTAGTGLIEGPG
                     LALFDASLGKNFLLTERFNLKFQGDFFNALNRTNFSTLNTNLSNTNFGTLSSANPPRQ
                     VQLSLKLSF"
BASE COUNT          845 a         1084 c          852 g          763 t
ORIGIN      
        1 tagcaatgta acctgttccg gtctgcggta cgccgttctc caaaaagacg gccgtaccac
       61 ttagcggtat agacatgtgg ccaatggatt gacagtcatt tttctgcgcc tgtacttact
      121 cctatagtcc gcaattcagt aactctgcgg atacaaagac aagcgtttgt attgcgggag
      181 ggtagtacag tgatgcagtc cggaaatcat ctcctgaaat ctgttttcgt ggcagtggtc
      241 atcgcggccg ctgcgtgttt cctttcggtc tcagcatctg cacagaccag cttcggccgt
      301 atctccggca cagtctccgc cccggatggc tctgtcgtcc ctggcgctca ggtcatcgta
      361 aaaaatacag aaacacaact cacacgccag acaaccaccg accagcaagg cttttacgtc
      421 ttcaccgagc tacccatcgg cacatacagc gtagaagtca atcagaccgg attcagcaaa
      481 tccacgcgca gcggtttagt gctcgttgcc gacggacgcc ttactgccga ctttacttta
      541 gtagtaggca gcaccagcca gaccgttgaa gtacagggca ccaatactga aacgctgaat
      601 acaacttcag gtgagctttc gcgcgtcatt gatacaaaac aggtggcgaa ccttccactg
      661 aatgggcgca attacattca gcttatgacg ctcgtgcccg gcgcagtcgt cacaaatcca
      721 gaccagttca gcgtgaccac gagccttgcg gccaataacc agaccatcaa tggcaaccgt
      781 ggcgacagca ataatctcac tgtagacggt gcatacaaca tggttgcagg cagcaataca
      841 agcctcatga acaacgtcag cgctgagttc atcaacgaag tcaagctaca gacctcgaat
      901 ttttccgccg agtacggacg tatgtccggc cccgcattca acatcgtcac caaaaacggc
      961 accaacgact ttcacggttc gctgtttgaa tatttccgca acgatgtcct ggacgcgacc
     1021 aactattttg ctccttcaaa aacagcattg cgcttcaacg atttcgggta tagcctgggc
     1081 ggccccatca ttcataacaa gctgttcttt ttcgtgggcg aagaatggaa gcggttgcgc
     1141 cagaaccaga ccacgcagcg gcagacaatt cctgattccg cattcctcag cggcgatttt
     1201 agcgcgctcc tgcctaagac gcagttgtat tatcccggca ccaaaacgcc catcccgaac
     1261 aacaatgtct ccaacctcat tacccctgac ggaaaagcga ttgccaatgt atatgccctg
     1321 atggcgaaga caggggcaaa atttgtagat gctccggtgg ccaacaatct cacccgctga
     1381 atcccctcaa ctatcgcgaa gatatcgtcc gtctcgatta tgtcttcagt cagaaaaatt
     1441 ttgcctacgg acgctggata caggactaca acagcctcat tgatcccttc ggcacatttt
     1501 caggatcgaa cctgccgact acgcccacaa cgcgcaaccg tcccggcgaa agcattctct
     1561 ttgccgagac ctggataccg actcccaact ttgtgaatga agcgcgtgtg aatggaagct
     1621 gggtctcgca gcacatcatt ccttacggca cggcatggca gcgtggcacc tatggcttcc
     1681 agtatccgct gctctatgga ggcgcagatt atcccaacgg catccccaac gtctcggtaa
     1741 cgggattcgc aaattttcaa gggccatact ttgcgttgaa gtctccttca accgatatcc
     1801 agatcgccga cacaatttcc tggacgcatg gtcagcatgt cttgcgcgcc ggggccgtgc
     1861 tcatccgcga cagagtagac cagaatggcc ggtcgcttta tgacggaagc atttccttca
     1921 acgccagcaa caaccctgaa tccaccggct acgccatggc agatgccctg cttggaaact
     1981 tcagctccta caccgaagcc agcgccgatc cggtcggctt cttccgcttc tggcagccag
     2041 aagccttcgt gcaggacacg tggcacgtga cccccaagct cagtcttgaa cttggcctgc
     2101 gctggcaata catgcaggca atctatacac aggcaaacaa cactgcgaat tttgacccat
     2161 cacgttacga ccccaaccag gccgtgcaga ttacgcatga cagcaagggc ctcatcgtgc
     2221 ccaactccgg aaacccgtac aacggccttg tgcgcgcggg cagcggcatt cctaaagacc
     2281 agacatcgcg cgtcccggga gcgaacgggc cgctctactc ggtcattcct gcgggagcac
     2341 cgcgcggcct ctatcctggt tctaacaact ttgctccgcg cctcggcttt gcctatgccg
     2401 tggatgacaa gaccaccctt cgtggcgggt tcggactctt ctataaccgc gccgaaggca
     2461 acattgtttt ctcgcaggtg aacattccgc catttctgca gatttccact tatagcaaca
     2521 gcaatctctc gaacatcacc ggcggctcca catcgcaggc gccgctcggt ggaatctccg
     2581 cgattgatcc ccacctgaag agccagactg tcgcgcaata cagcctgaat cttcagcatg
     2641 agatgccctg gggacttctg gcagagcttt cctatgtagg cacagaaggc cgccacatgc
     2701 tccgtcagcc aaacattaac taccctgacc tcagccaggt cgcagccaac cctaaggtct
     2761 caaccaatgc ctttgttcca tacaaaggat tcagctccat tacgcagatc cgcagtgact
     2821 ccactgcaaa ctacaatgcg cttcaggcat atgtatccaa acgcatggga gcattgcagt
     2881 tgaccgctgg atatacctgg tccaaagcgc tcgctgattc gtcgagtgaa agcgacaaca
     2941 gcgagaactg gacggtgcgc cattacaact atgggccaac ctcgttcgat cgccggaatg
     3001 ctttcttcag cacgttcgtg tggcagatgc cgaaactggc cgggcacaat gcctgggtgc
     3061 gcagcactgc cggcggatgg caactgagcg gcgtagcaag attgcagtcc gggcagtact
     3121 acaccgtcac cgggaccaca gccaccggca ctcgccgcgc agattacctg ggtggatcac
     3181 tgcacgtcaa tcatcccggg attcaaaaat ggttcaatac ctcggccttt gccgtggcgc
     3241 ccaatggtcg cttcggcacc gctggcacag ggctgatcga aggcccgggg cttgcgctgt
     3301 tcgatgcttc gctgggtaag aactttctgc tcacagaacg cttcaatctg aagtttcagg
     3361 gagatttctt caacgcgctg aaccgcacca acttcagcac gctgaataca aatctctcaa
     3421 acaccaactt tggaactctt tccagcgcaa atcctccgcg ccaggtgcaa ttaagcctga
     3481 agctgagctt ttagagcgcc atcacatgcc gggtcagatg gcccgggctt cccatccttc
     3541 gcgc
//
LOCUS       DASRBV010000191         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_10391,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000191 DASRBV010000000
VERSION     DASRBV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10391"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFA02_07435"
     CDS             <1..891
                     /locus_tag="VFA02_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU09720.1"
                     /translation="KRSVDWLHGTGCRVLAYMGGEPLLRPDLIHRITYYAAKKGFWIY
                     LPTNARLLRTEVIDKLADAGISTFNIAVDAVDVKPGLPKALKPIRKQFDYLVRKQYGY
                     GYSVFLNINICRNNLDDVRELTEIAHDNGIATDYHINESPLLEQDEHFKHQDDNVTYI
                     TKEDWPAVEETIQWLTEKQDAGYKMVNSNTRLWQMVDFMKGNHFPWNCRAGQNSMIIR
                     VDGTLAPCFPMYTAKYDWGVVGNHKFDTKQLDHQKETCQTHCFSTLNHILGWCYNDQR
                     VIKYFFKQLAHGFQGMKGQM"
     gene            965..2755
                     /locus_tag="VFA02_07440"
     CDS             965..2755
                     /locus_tag="VFA02_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09721.1"
                     /translation="MPAAANEVVQKDFEMEIQARIEAERARLRAEAGLKRMREFQRPV
                     ERTFTAEERDRVTILFGGLTWKHEEMIKAVFRGSGYHCENIPTPVVADFQAGKEYGNN
                     GQCNPTYFTVGNLVRYLQGLEKQGLTRQQIIDNYVFFTAGSCGPCRFGMYEAEYRYAL
                     QNAGFDGFRVLLFQQTDGIKAASGEPGLKFSVDFGMGMLNALNLGDVINELAYQIRPF
                     EVNKGETDRVIQEAVKTLTDTLRDRRRWHIMDAAPDWAKPYLEKHKKIEAIGCTLGKI
                     GHNLYGKEYVDALHACRDTIDSIQVDRLRVKPVIKITGEFWAQTTEGDGNFNMFAFLE
                     REGAQVLVEPIATWIAYMMYVAKEGAKARAEVEAPYKDPKWWELKKQLVNDLKLFKKT
                     GGLTVGSALWTYFYHRTIKHMGGTVHHLVPQKELARLAHPFYHQLARGGEGFMEVGKN
                     VYYTVNHLCHMVLALKPFGCMPSTQSDGVQSRVVNKFKDMIFLPIETSGEGEVNAHSR
                     VQMALAEAKAKARAEFQSVLDKTGKSLDDLREYVAEHPELSRALYNVPHQPGIAGTAA
                     QFASHVSDLMNKDRAYRHRARVSMATANAA"
     gene            2771..2953
                     /locus_tag="VFA02_07445"
     CDS             2771..2953
                     /locus_tag="VFA02_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09722.1"
                     /translation="MRSIIRAVVEGCLGAAVVAVAALLHIAVRSALRQQAEKDSDGFG
                     KGNSKRPVFLSRADEL"
     gene            2970..>3696
                     /locus_tag="VFA02_07450"
     CDS             2970..>3696
                     /locus_tag="VFA02_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZU09723.1"
                     /translation="MQQLLVGLDVGSTTVKAIVVDAATDAILWQDYQRHETRQPEKVL
                     EFLRRMELEAGITQENCRIFVTGSGGGTLADLIGAKFVQEVHAVSLAVEKLHPEVYSV
                     IELGGQDAKIIVFKDDEETGRKKKIPSMNDKCAGGTGAVIDKINAKLKIPAAELANQG
                     YHGIKLHKVAGKCGVFAETDINGLQKTGTPSHELMASLFEAIVLQNLSVLTRGNTLRP
                     HVLLLGGPNTFIRGMREAWQANIP"
BASE COUNT          935 a         1021 c          982 g          758 t
ORIGIN      
        1 aaacgctccg tagactggct ccatggcacc ggctgccgcg ttctcgctta catgggcgga
       61 gaacctttac tgcgtccgga cctgatccat cgcatcactt actatgcagc gaaaaaaggc
      121 ttctggattt atcttccaac caatgcccgc ctgctgcgta cagaagtcat tgataaactg
      181 gccgatgccg gtatttctac cttcaacatt gcagtggatg cggtggatgt gaaaccgggg
      241 ctgcccaaag cgctcaaacc catacgcaaa caatttgatt atcttgtgcg caagcaatat
      301 gggtacggct actcggtctt tctgaatatc aatatctgcc gcaataactt agacgatgtg
      361 cgcgagctga ccgagattgc ccacgacaac ggcatagcca ccgactacca catcaacgag
      421 tcgcctttgc tggaacagga cgaacacttc aagcaccagg acgacaacgt cacctacatc
      481 accaaagaag actggcccgc cgtcgaggaa accatccagt ggttgacaga gaaacaggat
      541 gcggggtaca agatggtcaa ttcaaatacg cgcctctggc agatggtgga cttcatgaag
      601 ggcaatcatt tcccctggaa ttgccgcgca ggccagaact cgatgattat tcgcgtggac
      661 gggacactag cgccctgctt tcccatgtac acggcgaaat atgactgggg cgtagtcggc
      721 aaccataagt tcgacacgaa gcagttggac catcagaaag agacctgcca gacccactgc
      781 ttctcaacac tgaatcacat tctgggctgg tgctataacg accaacgcgt cattaagtac
      841 ttctttaagc agttagcgca cgggtttcag ggcatgaagg gacagatgta aaaatctgtt
      901 ccttttcagt tttatcctgg aagtcttctg catctaagtt actcatcccc taaggagaat
      961 ctttatgccg gcagccgcaa acgaagttgt tcagaaagac tttgagatgg aaatccaggc
     1021 gcgcattgag gccgagcgcg cgcgccttcg cgcagaagcg ggtctgaaac gtatgcgcga
     1081 attccagcgg ccagtggaac gcaccttcac tgcagaagag cgcgaccgcg tgaccatcct
     1141 ctttggcggc ctgacatgga agcacgaaga gatgatcaag gccgtctttc gcggcagcgg
     1201 ctatcactgc gaaaacattc ctactcctgt tgtcgccgat ttccaggccg gcaaggaata
     1261 cggcaacaac ggccaatgca atcccactta cttcacggtc ggcaacctgg tccgctattt
     1321 acaaggactg gagaaacaag gtcttactcg tcagcaaatc attgataatt acgtcttctt
     1381 cactgctggc tcctgcggcc cctgccggtt cggcatgtat gaagccgaat atcgctacgc
     1441 cttacagaat gcgggcttcg acggcttccg cgtgctgctc tttcagcaga ccgatggcat
     1501 taaggcagcg agcggcgaac cgggactgaa gttctccgtt gactttggaa tggggatgct
     1561 caacgcgctg aatctgggtg acgtaatcaa tgagcttgcg taccagattc gtccctttga
     1621 agtaaataaa ggcgagaccg accgcgtgat tcaggaagcg gtaaagacac tgaccgacac
     1681 gctgcgcgac cgcaggcgct ggcacatcat ggacgcagct ccggattggg ccaaacccta
     1741 tcttgaaaaa cacaagaaga tagaagccat cggctgcacg cttggcaaaa tcggacacaa
     1801 cctttacggc aaagaatatg tggacgcact ccatgcctgc cgcgacacca ttgattccat
     1861 tcaggtagac cgcctgcgcg tgaagccagt catcaaaatt accggcgagt tttgggcgca
     1921 gaccactgaa ggcgatggca acttcaatat gttcgctttc ctcgaacgtg aaggcgcgca
     1981 ggtattagta gaaccaatcg ccacctggat tgcctacatg atgtacgtag ccaaagaagg
     2041 cgcaaaggcg cgggcagaag tggaagctcc atacaaagac ccgaagtggt gggaactcaa
     2101 gaaacagttg gtcaacgatc tgaagctatt taaaaaaaca ggtggcctga ctgtcggcag
     2161 cgcgttgtgg acctacttct atcaccgcac catcaagcac atgggcggca cggtgcatca
     2221 cctcgtccca cagaaggagc tggcgcgtct ggcgcatccc ttctatcacc agcttgcgcg
     2281 cggcggcgaa ggctttatgg aggttggcaa aaacgtctat tacaccgtga accacctctg
     2341 ccatatggtc ctggcgctca agcccttcgg ctgcatgcct tccacgcagt ctgatggcgt
     2401 gcaatcgcgc gtggtcaaca aattcaagga catgatcttc ctgcccattg aaacttccgg
     2461 cgaaggcgaa gtgaacgcgc acagccgcgt gcagatggcc cttgccgaag caaaagccaa
     2521 agcccgcgct gagttccaga gcgtgctcga caagacaggt aaatcgctcg acgacctgcg
     2581 tgagtatgtt gcggagcatc cagaactatc ccgtgcgctt tataacgtgc cacaccagcc
     2641 cggtatcgca ggaacagccg ctcagtttgc ctcgcatgtc agtgacttaa tgaataagga
     2701 cagggcatac cggcatcgtg cccgcgtttc gatggcaaca gcaaacgcag cataacaagt
     2761 ggagttacat atgcgctcaa tcatacgggc cgttgtagaa ggatgcctcg gggcagccgt
     2821 ggtggccgta gcagcacttt tgcacattgc cgtgcgcagt gcgctgcgcc agcaagccga
     2881 aaaagacagc gacggttttg gcaagggcaa tagcaaacgg cccgtgtttt tgtcgcgcgc
     2941 cgatgaactg tgaactttag gagatacgga tgcagcagct tctggttggt ctggacgtag
     3001 gctcgacaac agtaaaagcc attgtggtgg atgccgccac tgacgccatc ctctggcaag
     3061 actatcagcg ccacgaaaca aggcagcctg aaaaagtgct cgaattcctg cgccgtatgg
     3121 aattagaggc cggaattaca caggaaaact gccgcatctt cgtcaccggc tccggcggcg
     3181 ggacactcgc tgacctcatc ggcgcaaaat ttgtgcagga agtccatgcc gtttcgcttg
     3241 ccgtcgagaa gctgcatccc gaagtctatt cggtgatcga acttggcggt caggacgcga
     3301 agatcatcgt ctttaaagat gacgaagaga ccgggcgcaa aaagaaaatt ccttccatga
     3361 acgacaagtg cgccggtggc acgggcgccg tcattgacaa gatcaacgcc aaactcaaga
     3421 ttcccgcggc agagcttgcc aaccagggat atcacggcat caagctgcac aaagttgccg
     3481 gcaaatgcgg tgtctttgcg gaaaccgaca ttaatggcct gcagaaaaca ggtactccct
     3541 cgcacgaatt gatggcttcc ctctttgaag ccatcgtctt gcagaacctc agtgttctca
     3601 cacgcggaaa tacgcttcgt ccgcacgttc ttctgctcgg agggcccaac accttcattc
     3661 gcggcatgcg cgaggcgtgg caggcgaaca ttccgc
//
LOCUS       DASRBV010000192         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_571127, whole genome shotgun sequence.
ACCESSION   DASRBV010000192 DASRBV010000000
VERSION     DASRBV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571127"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFA02_07455"
     CDS             complement(<1..873)
                     /locus_tag="VFA02_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU09724.1"
                     /translation="MSETAKVLGKVGLPAPAKELAQRMWDVIIVGAGHNGLACAAYLA
                     RAGKRVLVLEARERVGGACTIEEPFPGVRMSPCAYLAGLLHPLVVKELNLPQRGFAWS
                     PAINGLFVPFLDGTSIQLWDDDAKCEEEVRRFSSRDLNGWKAMCDVIRRLRDALRPAG
                     DGDLWIGDAPTREQMEERLGQDEEARSVLFSWSMAEFVEHYLQDERLHIALLGQGVIG
                     TNASPFDAGTASIRFHHSSGRLGGMPGMWGYVKGGMGMVSFYFCDAAREAGAVVATGV
                     PVAAILPGEGVLLEG"
     gene            complement(889..1437)
                     /gene="thpR"
                     /locus_tag="VFA02_07460"
     CDS             complement(889..1437)
                     /gene="thpR"
                     /locus_tag="VFA02_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZU09725.1"
                     /translation="MRLFTGLALDEKTTDISGAFVSRIQRGFPTLRWSPPQQWHVTLQ
                     FLGEVKDDRYSCIVERLQAVRTHAVEVRLAAPDFFERAGVFHIAIEKTETLLALHRVT
                     QQALLQCGFVPEARPYSPHITLARNKGRAPSSDLERLRREVEKHAPVKFPPFVAREFL
                     LYQSFTEPSGPRYEVRERFALM"
     gene            complement(1443..4073)
                     /locus_tag="VFA02_07465"
     CDS             complement(1443..4073)
                     /locus_tag="VFA02_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HZU09726.1"
                     /translation="MLRKTASFFAALVLGCTAFFTANADALREGWSVQSACKLQTDGA
                     GISTTAYHPEGWYKTSVPATVLTVQVNAGVYQQPYFGTNLRNIPGTTYPIGRIFANLP
                     MPEDSPYRCGWWYRKEFVVPAARKGRTVWLRFAGINYRADVWLNGKRIADSTQVAGAY
                     RTYEFDVTDKIVYGKPNVLAVETFAPSETDLGINWVDWNPAPPDKDMGLWGAVDLTTS
                     GPVTLRSPMVVTHFTDASLKQANLTVYAELHNATDRPVHGNVTGTVANIRIEQPVNLA
                     PHEDKTVVFHPEQFTQLKLKDPQIWWPYQMGTPHLEVLSLRFVTRGKVSDEQSVRFGI
                     REITSELTGKGHRLFRVNGKPILVRGAGWSPDMLLREDPQRLREQFMLVRDMHLNTIR
                     LEGKLETEDFFRLADEQGILIMLGWCCCDQWEHWSKWSQENYQVARESLRSQMLRIRS
                     HASLLVWLNGSDNPPPPDVERMYLDVEAQTHWPNPILSSASARSTTVTGASGVKMTGP
                     YDYVAPSYWYVDKGKHGGAWGFNTETSSGPAIPTISSLRKFLPANQMWPTDTPSWDFH
                     NGSGKFSRLTVFNEAMNAAYGAPQDLKTYVRIAQTMAYDSERSMFEAYTRNKYTSTGV
                     IQWMLNNAWPSNIWHLYDYYLDAGGGYYGTKKACEPLHVQYSYDDHSIVVVNSTYQPS
                     PRLIAEASVYDINLKKLFSKVQELTIAADTSQRTILIPDPVFANPSSLYFVDLTLRKE
                     RGEIISRNFYWIPSALTTFDWPKTDYTHTPALQHEDMKSLGNMPKAQVQAHITSMKNR
                     TVTLHLQNPSDALAFQVSVSVRNADGEDITPVIWSDNYLELMPGESRILTAQLPAGTP
                     ESATVVISGWNIAEHAIPLR"
     gene            complement(4150..4308)
                     /locus_tag="VFA02_07470"
     CDS             complement(4150..4308)
                     /locus_tag="VFA02_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09727.1"
                     /translation="MERYMDRRDFIKTAVWVRAKEKAGTARLTAKHPRLGNQTVEIAL
                     RHAPKELL"
     gene            4477..>4588
                     /locus_tag="VFA02_07475"
     CDS             4477..>4588
                     /locus_tag="VFA02_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZU09728.1"
                     /translation="MSPFTRREFLKGSLAAGTVATVGALPLSAARRTATDM"
BASE COUNT          982 a         1299 c         1254 g         1053 t
ORIGIN      
        1 tccctcaagc agtacgccct cgccgggcag aattgcagct accgggacgc ctgtggcaac
       61 gacagcgccg gcctcgcgcg cggcgtcgca gaagtaaaac gagaccatgc ccattccgcc
      121 ctttacgtat ccccacatgc caggcattcc gcccagccgt ccggaagagt gatgaaagcg
      181 aatcgaagca gtaccagcat cgaatgggct ggcgttggtg ccaatcactc cttgcccaag
      241 aagagcaatg tgcaggcgct cgtcctgaag atagtgctcg acgaactccg ccatagacca
      301 ggagaagagc acgctcctcg cttcttcgtc ctgtcccaga cgctcttcca tctgctcgcg
      361 tgtgggtgca tcgccaatcc acaggtcgcc atcgcctgct ggacgcaggg catcgcgcag
      421 ccggcggatg acatcgcaca ttgccttcca gccattcaga tcgcggctgc tgaagcggcg
      481 gacttcctct tcgcattttg cgtcgtcgtc ccagagttgg atgcttgttc catccagaaa
      541 tggcacaaac aggccattga ttgccgggct ccacgcaaaa ccgcgttgcg gtagattgag
      601 ttctttcact accagtggat gcagcagtcc ggccagatac gcgcagggag acatgcgcac
      661 tccgggaaat ggctcctcaa tggtgcatgc tcctcctacg cgttcgcgcg cttcgagcac
      721 cagcacacgc tttccagcac gcgcaagata tgcggcgcac gcaagaccgt tgtggcctgc
      781 acctacgata atgacatccc acatacgctg cgccagttct ttcgccggcg cgggcaggcc
      841 gacttttccc aggacctttg ctgtttctga catgcgctgc aatgcaggtt acatcaaagc
      901 gaagcgctcg cgcacttcat accttggccc ggatggttcc gtgaagctct ggtacagtaa
      961 aaattctctt gcgacaaaag gaggaaattt taccggagcg tgtttttcta cttcgcgccg
     1021 cagccgctca aggtctgaag aaggagcacg tcccttgttg cgcgcaagcg tgatgtgcgg
     1081 agagtacggc cgcgcttccg gcacaaagcc gcattgcagc agggcctgtt gcgttacgcg
     1141 atgcagcgca agcagcgttt cagttttctc aatggctatg tgaaacactc ccgcacgctc
     1201 aaagaaatcc ggcgcagcaa gccgcacttc caccgcatgg gtgcggacgg cttgcagtct
     1261 ctccacaatg caactgtaac ggtcatcttt gacttcgccg agaaactgca aggtcacgtg
     1321 ccattgctgt ggaggcgacc accgcagagt gggaaacccg cgttggatcc tgcttacaaa
     1381 tgcgcctgaa atgtccgtcg ttttctcgtc cagcgccaga cctgtaaaaa gccgcataga
     1441 tttcacctga gcggaattgc atgttccgca atgttccatc cggagatgac gaccgttgca
     1501 ctttccggag tgcctgcggg aagctgcgcg gtaaggatgc gcgattctcc cggcatcagc
     1561 tcaagataat tatctgacca gatgaccggc gtgatgtctt ctccgtctgc attgcggacc
     1621 gacaccgaaa cctgaaaggc cagtgcgtcc gacggattct gtagatgcaa tgtgacggtc
     1681 ctgtttttca tgcttgtgat gtgtgcctgc acttgcgctt ttggcatgtt tccgagggac
     1741 ttcatgtctt catgctgaag cgccggagtg tgcgtgtaat cagtctttgg ccagtcgaag
     1801 gtcgtaagag cggaaggtat ccagtaaaaa ttgcggctga tgatctcgcc tcgctctttc
     1861 ctcagcgtca ggtcaacgaa atacaggctc gaagggttgg caaagaccgg atccgggatc
     1921 agaatggtgc gctgtgaagt gtctgcagcg atggtaagtt cctgcacttt tgaaaacagc
     1981 ttcttcaggt tgatgtcata cacacttgct tctgcaatga gacgcggcga cggctgataa
     2041 gtgctgttca cgactacaat actgtgatcg tcataggaat actgcacatg cagcggctcg
     2101 catgctttct ttgtgccgta ataaccgcca cctgcgtcga gataatagtc atacagatgc
     2161 catatgttgg aaggccatgc attattgagc atccactgga tgacgcccgt ggatgtgtac
     2221 ttgttgcgcg tgtaggcctc aaacatagag cgctcgctgt cataggccat cgtctgtgcg
     2281 atgcgcacgt aggtctttaa atcctgtgga gcgccatagg cggcgttcat cgcctcattg
     2341 aaaacggtaa gacgcgaaaa ttttccgctg ccgttatgga agtcccatga aggagtatct
     2401 gtaggccaca tctggttcgc gggcagaaac ttcctcaggc tgctgatggt gggaatcgcc
     2461 gggccggaac tggtttccgt gttaaagccc cacgcgccgc catgtttgcc tttgtccaca
     2521 taccagtaag atggcgcaac gtaatcataa ggccccgtca tcttcactcc gcttgcgcca
     2581 gtgacggtag tagagcgcgc cgatgctgat gacaggatgg gattgggcca gtgcgtctgc
     2641 gcttccacgt ccagatacat gcgttccaca tcgggaggag gagggttgtc gcttccattc
     2701 agccacacga gcaggcttgc atggctgcga atacgcagca tctgtgaacg cagcgattct
     2761 ctggcgacct gataattctc ctgactccac ttgctccagt gctcccattg gtcgcagcag
     2821 caccagccca gcatgatcaa aatcccctgc tcgtcggcca gacggaagaa gtcttctgtt
     2881 tccagttttc cttccagccg aatggtgttc aggtgcatgt cgcgcaccag catgaactgc
     2941 tcccgcaacc gctgcgggtc ttcgcgcaac agcatgtctg gcgaccagcc cgcgccgcga
     3001 acaagaatgg gtttgccatt gacgcgaaaa aggcgatggc ccttgccggt aagctccgac
     3061 gtaatctcac ggatgccaaa gcgcacagat tgctcatcgg agaccttgcc acgcgttaca
     3121 aagcgcagac tgagcacctc cagatgcggc gtgcccatct gatacggcca ccagatctgc
     3181 gggtctttca atttgagttg tgtgaactgt tcaggatgaa agacaacggt tttgtcttcg
     3241 tgcggcgcta ggttcaccgg ttgttcaatc ctgatatttg caactgttcc agttacattc
     3301 ccatgcaccg gtctgtctgt ggcgttgtgc agctcagcgt acacggtaag attggcctgt
     3361 tttagcgatg catctgtgaa gtgcgtcacg accatgggcg agcggagggt caccgggccg
     3421 ctggtcgtga ggtctacagc accccacaag cccatgtctt tatctggcgg agcggggttc
     3481 cagtccaccc agttaatgcc gaggtcggtc tcggaaggcg cgaatgtttc cacggcaaga
     3541 acatttggct tgccgtagac aattttgtct gtcacatcga attcgtacgt gcgatatgcg
     3601 cctgcaacct gcgttgagtc ggcaatgcgt ttgccgttaa gccagacatc ggcacgatag
     3661 ttgattccgg caaaacgcag ccataccgtg cgtcctttcc gggcagcagg aacaacgaat
     3721 tccttgcgat accaccatcc gcagcggtaa ggactgtctt ccggcatggg caggttcgca
     3781 aagatgcggc cgattggata ggtagttcct ggaatattac gcaggtttgt gccgaagtag
     3841 ggctgttgat aaacgcctgc attgacctgt accgtcagca cagttgcagg cacgctggtt
     3901 ttgtaccagc cttccggatg atacgcagtg gtggagattc ctgcgccatc ggtctgcaat
     3961 ttgcaggccg actgcacgct ccatccttca cgaagggcat ctgcattcgc cgtgaaaaaa
     4021 gcggtacagc ccaggacaag cgcggcaaaa aaggatgcag ttttccgcaa catgcagagg
     4081 tcttcctccg gagatgctga ctcaggaatt taaacttcca tcctaactgc acaatgcgga
     4141 gttcgtgcgc taaagcagct ctttcggggc gtgcctgagc gcaatttcca ctgtctggtt
     4201 gccgagccgt ggatgtttcg ctgtcagccg cgccgtgccg gctttttctt tggcgcggac
     4261 ccagacggcc gtctttatga agtcacgcct gtccatgtac cgttccatga aatgaaagcg
     4321 ttttcatgct ttcaaaaaaa acaatacatg aatcggcccg ctttatgcac tgtctcgctg
     4381 aaatttagaa cctgagagca tcgctgcctt gcagcctgtt gcactacagc gatacgattc
     4441 acacaatagg cccgccatct cgtccaggag attgatatga gtccgtttac ccgccgggaa
     4501 tttctaaaag gatcgttggc agcaggcaca gtggccacgg tcggcgctct tcctctctcc
     4561 gcagcccgtc gcaccgcgac tgacatgg
//
LOCUS       DASRBV010000193         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_620263, whole genome shotgun sequence.
ACCESSION   DASRBV010000193 DASRBV010000000
VERSION     DASRBV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620263"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..949
                     /gene="rfbA"
                     /locus_tag="VFA02_07480"
     CDS             62..949
                     /gene="rfbA"
                     /locus_tag="VFA02_07480"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZU09729.1"
                     /translation="MKGIVLAGGSGTRLYPVTHVVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDTSRFADLLGTGEQWGVSFSYAVQPSPDGLAQAFLIGKEFIGKDPC
                     ALVLGDNIFYGQSLSGQLQRAAKKTSGATVFAYPVNDPERYGVVEFDAQGRAISLEEK
                     PVKPKSRYAVTGLYFYDNDVVGLASGLKPSARGELEITDLNRCYLERGTLDVEIMGRG
                     MAWLDTGTHDSLFEAGLFIQTIEHRQGLKVACPEEIAFRSGYITAAQLEKLAQPVRKS
                     GYGQYLLSLLNEPYFPANA"
     gene            960..1505
                     /gene="rfbC"
                     /locus_tag="VFA02_07485"
     CDS             960..1505
                     /gene="rfbC"
                     /locus_tag="VFA02_07485"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZU09730.1"
                     /translation="MNVIETALPGVLILEPRIFRDARGAFCETYNERAMTALGLPTHW
                     VQDNFSVSRKNVVRGIHYQIQNTQGKLVRVTHGVVWDVAVDLRRSSPAFGQHVGVELS
                     GENGRMFWIPEGFGHGFVTLSEVAGFAYKVTDYYSPAGERTLLWNDPELGIEWPVSAA
                     EAIVSEKDQQGRPLREAELFP"
     gene            1502..2353
                     /gene="rfbD"
                     /locus_tag="VFA02_07490"
     CDS             1502..2353
                     /gene="rfbD"
                     /locus_tag="VFA02_07490"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZU09731.1"
                     /translation="MSQRKFLLLGAKGQVGTELRRSFAGAGEVIACDRRQADLSQPEG
                     LRELVREVRPDVILNAAAYTAVDRAESEPELAMTINATAPRVLAEEAAKTGALLVHYS
                     TDYVFDGTKDGPWIETDATNPLSIYGKTKLAGERAIQEAGGTYLIFRTSWVFGPHGHN
                     FLLTMLRLGKERPQLRIVDDQIGAPTSSSEIAKATRAVLEEFTSAKRGIYHMTCSGQT
                     SWYGFARAIFADAPAPEIIPIPSAEYPTPAKRPRNSVLSNEKLKATFGVGLSGWQEAL
                     RQALQSL"
     gene            complement(2354..3367)
                     /locus_tag="VFA02_07495"
     CDS             complement(2354..3367)
                     /locus_tag="VFA02_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU09732.1"
                     /translation="MKTVVSRKILICALSAAFSISFAAAQTTPPAPVQTQQQPAPNAS
                     QPQPAHANGQQPQDQEYTITTSANEVNLVFTVTDKHGRFIKDLKLNDFALLDDQKAPA
                     QVFSFTQQTNLPLRVGIVIDTSTSIRQRFQFEQQSASEFLLQILRPKTDKAFVMGFDV
                     TPDYKQDWTNNLDLLNTGINALQPGGGTALFDAVYSACRDKMLDAAREREPVRRAMIL
                     VSDGNDNQSHAYLDDAIKMCQRAQTIVYTISTNTSPSRDRGDDVLEKIALATGGRSFF
                     PKRMEDMYSNFQDIEDELRSQYSLVYKPADFVANGAFRTIYLFCLDRKYTVRASKGYF
                     ATK"
     gene            3476..>4347
                     /locus_tag="VFA02_07500"
     CDS             3476..>4347
                     /locus_tag="VFA02_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU09733.1"
                     /translation="MKKIRFLFLAAFALLMLDVPACLSQTPVPAPSAADILRQLPKPI
                     FLWPNGAPGALGDTEADKPRLYPFVPKKKSTTAAVLVIPGGGYQHVAIGHEGFQYALW
                     LNDQGVTAFVLDYRVAPYKYPVEIQDGMRAMRYVRAHASEFGVDPNRIGVWGSSAGGH
                     LASTLGVHGNDAAPPPDNDPFNQTDAKPDFMVLSYPVVSMELPETHAGSLHNLLGENP
                     DSALAEKLSNQLTANAQTPPTFLFATTRDPAVPVENSLDLYRTLERAHVPVEMHLYDY
                     ANHGCGLCGDIPELS"
BASE COUNT          900 a         1115 c         1332 g         1000 t
ORIGIN      
        1 atgtgcgttc gggtgcgtat ctcaaatgga ttgaaaccaa ctatgcgggg cggactgcat
       61 gatgaagggg attgtgctgg caggcggttc gggaacgcgc ctctatccgg tgacacatgt
      121 cgtttccaag caactgcttc ccgtctatga caagccgatg atttattacc cgctgagtac
      181 gctgatgctt gccgggattc gcgacattct gattatttcc accccgcagg acacttcgcg
      241 ctttgccgat cttctgggga caggcgaaca gtggggcgtc agcttttctt atgccgtgca
      301 gccgagtcct gatggactgg cgcaggcctt tcttattgga aaagagttta tcggcaaaga
      361 tccctgcgca ctggtactgg gcgacaacat cttttatggc cagtcgctct ccgggcaatt
      421 acaacgggcc gcgaaaaaga cctccggagc gacggtcttt gcctatcctg tgaatgaccc
      481 ggagcgatat ggcgtagtgg aattcgatgc gcaggggcgc gccatcagcc tggaagaaaa
      541 gccagtcaag ccaaagtcgc gctacgcggt tacggggctt tatttctatg acaacgatgt
      601 ggtggggctg gcgtcgggtt tgaaaccttc agcgcggggt gagcttgaga tcacagacct
      661 gaaccgctgt tatctggaac gcgggacact ggatgtggag atcatgggcc gcggtatggc
      721 atggctggac acaggaacgc acgactcgct ttttgaggcc gggctgttta ttcagaccat
      781 cgagcaccgg caggggctga aggtcgcctg cccggaggag atcgccttcc gctccggata
      841 catcacggct gcgcaacttg aaaagctggc gcagccagtc cgcaagagtg gatacgggca
      901 gtatcttctg tcccttctga acgagccgta ttttccggcc aacgcatagg tttcccgtta
      961 tgaatgtgat tgaaactgca ttgcctggcg tgttgatact tgagccgcgt atcttcaggg
     1021 atgcgcgggg ggccttttgc gaaacctaca atgagcgtgc gatgacggcc ctggggctgc
     1081 ctacacactg ggtgcaggac aatttctctg tgtccagaaa gaacgtcgtg cgcggtattc
     1141 attatcagat ccagaacacg cagggaaagc tcgtgcgcgt gacccatgga gtggtctggg
     1201 acgtggccgt ggacctgcgg agaagttctc ctgcatttgg ccagcatgtg ggtgtggagc
     1261 tttccggtga aaatggacgc atgttctgga taccggaagg gtttgggcat ggatttgtaa
     1321 ctttgtcaga ggttgcaggg ttcgcctata aagtgaccga ctactactct cctgccggag
     1381 agcggacgtt gctgtggaat gatccggagc tggggattga gtggcctgta tctgccgcag
     1441 aggccattgt ctctgaaaaa gaccagcagg gaaggccgct gcgtgaagcg gagctatttc
     1501 catgagtcag cggaaattcc ttcttctggg agcaaaaggc caggtaggaa ctgagctgcg
     1561 gcgcagcttt gccggtgccg gggaagtgat tgcgtgcgac cgccggcagg ccgatctttc
     1621 ccagcccgag ggtctgcgtg agcttgtgcg cgaagtccgg cctgacgtca ttctgaatgc
     1681 ggcggcctat acggcggtgg accgggccga gtcagagccg gagctggcga tgacgattaa
     1741 tgccactgct ccgcgtgttc ttgccgaaga ggcagcaaaa acgggcgcat tactcgtgca
     1801 ttattcgacc gactacgtct ttgatggaac aaaagacggg ccgtggatcg agacagatgc
     1861 tacgaatccg ctgagtattt acggaaaaac caagctggcc ggcgagcggg cgatccagga
     1921 agccggtggt acatacctga tcttcaggac gagctgggtc tttggcccgc atggacataa
     1981 ctttctgctg acgatgctcc ggctgggcaa agaaaggccg cagttgcgta ttgtggatga
     2041 ccagattggc gcgcctactt cttccagtga gatagcaaag gccacacgcg ctgttcttga
     2101 agaatttacc agcgctaagc gcggcattta tcacatgacg tgctcggggc agacatcgtg
     2161 gtatggcttt gcgcgcgcta ttttcgcaga tgcacctgct ccggagatca tcccgattcc
     2221 atccgcggag tatccgactc cggcgaaacg cccccggaac tcagttctga gcaatgagaa
     2281 gctgaaggcc acttttggcg tggggctttc aggctggcag gaagcactgc ggcaggcact
     2341 gcagagtctc tgattattta gttgcaaaat accctttact ggcgcggacc gtatatttgc
     2401 ggtcgaggca gaaaagataa atggtgcgga aggccccgtt tgcgacaaag tcagcgggct
     2461 tatagaccag tgaatactgg ctgcgcaatt cgtcctcgat gtcctgaaaa ttgctgtaca
     2521 tgtcttccat gcgtttggga aagaaggagc gtccaccagt ggcgagggcg attttttcaa
     2581 gcacatcgtc gccgcggtcg cggctggggc tggtattggt gctgatggtg tagacgatgg
     2641 tctgcgcgcg ctggcacatt ttaatggcgt catccagata ggcatggctc tggttgtcat
     2701 tgccatccga gacaagaatc atcgccctgc ggaccggctc gcgttcgcgc gcagcgtcga
     2761 gcattttatc gcggcatgcg ctgtaaacgg cgtcaaacag ggcggtgccg ccgccgggtt
     2821 gcaatgcgtt gatgccggtg ttcagcagat caaggttgtt ggtccagtcc tgcttatagt
     2881 cgggcgtcac gtcaaagccc atcacaaagg ccttgtcggt cttcgggcgg agtatctgta
     2941 acaggaactc gcttgccgat tgctgctcaa attgaaagcg ctggcggatg gaggtgctgg
     3001 tgtcaatcac aatgccaacg cgcagcggca ggtttgtctg ctgggtaaag ctgaagacct
     3061 gcgccggggc cttctggtca tcaagcaggg cgaagtcatt gagcttcagg tctttaataa
     3121 agcggccgtg cttgtcagtg accgtgaaga ccagattgac ttcgttggcc gaggtcgtga
     3181 tggtgtattc ctggtcctgc ggctgctggc cgtttgcgtg cgcgggctga ggctggcttg
     3241 cattcggagc aggctgctgt tgcgtctgga ccggagcagg cggtgttgtc tgtgcagcag
     3301 caaatgagat agaaaaagct gcgctgaggg cgcagataag gattttccgg gaaacgaccg
     3361 tcttcattgt ggctgaggcc attctacata atgtcagggc gcagggcaca gggcaaagac
     3421 gagtcctgtc cggaaggcat ctgcttacag gaggacaaca gctatactgc tgacgatgaa
     3481 gaaaatccgt tttctgttcc ttgcagcctt tgcgcttctc atgttggacg ttcctgcttg
     3541 tctttcgcaa acacctgttc ccgcaccgtc agccgcggac attctgcggc aactacccaa
     3601 gccaatcttt ctgtggccga atggcgctcc aggcgctctg ggcgataccg aggcggacaa
     3661 accgcgcctc tatccttttg ttccgaaaaa gaaatcaacg actgctgctg ttcttgtgat
     3721 tccaggcggt ggatatcagc atgtggccat cggacatgaa ggttttcagt acgccctgtg
     3781 gctgaatgat caaggggtca cggcctttgt cctcgattat cgcgttgcac cttacaaata
     3841 tcccgttgag attcaggatg gaatgcgcgc catgcgttat gtgcgcgcgc acgccagtga
     3901 attcggagtt gatccaaacc ggattggtgt gtgggggtct tcggcaggag gccatctggc
     3961 gtccaccctc ggagtgcatg gcaacgacgc cgctccgcct ccggacaatg accctttcaa
     4021 tcagacggac gcaaagccgg attttatggt cctttcttat ccggttgtca gcatggaact
     4081 gcccgaaaca catgctggct cactgcacaa tctgctgggt gaaaatcctg attctgcttt
     4141 agcggagaaa ctttcgaacc agttgacggc aaacgcgcag accccgccga cttttctttt
     4201 tgctacaacc cgagatcccg cggtcccggt agaaaacagc cttgacctgt accgcacgct
     4261 ggagcgggcg catgtgcctg tcgagatgca cctgtatgac tacgcgaacc acgggtgcgg
     4321 cctgtgcggc gatattccgg agctttc
//
LOCUS       DASRBV010000194        13008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_725473, whole genome shotgun sequence.
ACCESSION   DASRBV010000194 DASRBV010000000
VERSION     DASRBV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725473"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFA02_07505"
     CDS             complement(<1..392)
                     /locus_tag="VFA02_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09734.1"
                     /translation="METSVIFRVLLLCAAAGGFQHVQATASALLREGVPAGGEVAPDV
                     VVVGEVVPRAEVVVAGDLLDAAVERAQDDIPAAGVAVVVQVSFPVSAAEQGGIPALAD
                     GLRYAAERPGAAAVGPGDACRDGSDWACS"
     gene            complement(435..1160)
                     /locus_tag="VFA02_07510"
     CDS             complement(435..1160)
                     /locus_tag="VFA02_07510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU09735.1"
                     /translation="MKNKPDYGVDAPGVLRNLFLFGVPCLLLGIFGPRAVHISHVIFL
                     LRPMFFWTGLFLILEGLLYLFYVKYGKFRHRDLMLSLYQWRGDETVLDIGCGRGLLLA
                     WAAKKLNSGHATGIDIWSKEDMAGNSEAATQRNLELEGVNSRCELVSVAAQEMPFPDA
                     SFNVVLSNLCLHNIYDRPARLRAVQQIARVLKPGGVAILSDYKNTKEYAEELQRAGLT
                     TEMHWGNPLYTFPPLRIVVARKA"
     gene            complement(1157..2233)
                     /locus_tag="VFA02_07515"
     CDS             complement(1157..2233)
                     /locus_tag="VFA02_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09736.1"
                     /translation="MTPSSLRVLYVVAETNPGSASLGRWNALQRMGLDGAWVVRTDEH
                     LPVSSIVDKIRFRTQFGPKVWKFNRFVLDTAVRNKANVLLADKPLSLTPGTLRRLRQQ
                     GVFLIDLFIDNPFGPRKDPGFRLFRKALPKFDLNAVQRKVSIDAFKAHGAKDVVLTLV
                     GFDRDVHFPSPSPVTDNERTRSVSFIGSPYDDRAEWFTSLFKAGVPLDISGPRAPWEK
                     ELAPEVMQAVFKNGPLRGKEYREALWKSKINLCFVTKSNQDEAAQRSYEITACGGFML
                     AERTPRHLELFKEDEEAVFFSTKEECIAKIQRYLPDEEARNRIAAAGCRRAWSSGYDH
                     DSLMRDLLMKAMEMMEHRKISSSK"
     gene            complement(2233..4281)
                     /gene="thrS"
                     /locus_tag="VFA02_07520"
     CDS             complement(2233..4281)
                     /gene="thrS"
                     /locus_tag="VFA02_07520"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZU09737.1"
                     /translation="MSSPVTSIRVQLPDGSQKELPAGSTPLDVAKSISPRLASAAVVA
                     RIRPLNGNGSTAAAAVLHETEGEAAMYSAENTHGERLVDLSAPLHEDVALEILTEKDP
                     DALKVLRHSAAHVLATAVLELFPETKLGHGPATDAGFFYDFYRPTPFTPEDLKKIEEK
                     MADVVARDEKFVREWEPREEGLQRFRADNDFMKVHFIERFTKPGEQISLYRNGAFVDF
                     CRGPHVPSTGRVKAFKVTNLAGAYWLGDEKNPQLQRIYGTAFFSRKELDEHFARLEEA
                     AKRDHRVLGKQLDLFSIQELAGSGLIFWHPKGAIIRKVMEDWMREECIRRGYDLVYTP
                     HVMRTELWKVSGHEGFYAQNMFTPMELDDADYRLKPMNCPGHILIYKNSPKSYRDLPV
                     RLAELGNVYRYERSGTMHGLLRVRGFTQDDAHIFCTPEQIEDEVVACIDFAQSVLKTF
                     GFSDFKVELSTWDPKDRQSYAGSDEKWNLAIRSLENALDRKGIPFKTIPGEAAFYGPK
                     IDVKLVDVLGRLWQLSTVQFDFNLPARFELEYVGEDGERHQPVMVHRALFGSVERFFG
                     VLIEHYAGAFPFWLAPVQVGLVPISERHVEYAEKIQQQLQNAGLRVELDARNEKMNAK
                     IRDFTMQKVPYVLVMGDKEAEAGAVSVRTRGKGDQGQMPLGEFLAKAANLVETKTTEL
                     "
     gene            4614..5792
                     /locus_tag="VFA02_07525"
     CDS             4614..5792
                     /locus_tag="VFA02_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZU09738.1"
                     /translation="MSLGVLNNISALYAANNLNQTQASLQNVLTQLSSGSRINSGADD
                     AAGLSLANGLTASATALSQSAKNASEGVDFLQVADGALSQVTNLLNRAVTLATEASNG
                     TLNAAQQAATQAEYSSIMTEISTINSNTEYNGINVFSNAANIYTSDGTVNQSYATSAL
                     GSTSSDSLKLGSVVYVSGNVAAATTTQAGKTDSYGNAIKAADNLLTATDPSGHNVVID
                     LTAMGDTTTAAGALSAATAVAISGGKALAGGVAAATAAGTDEYGSAYTTANQVFTSGA
                     TVINMGTTAPTSNEYVGTSLSNNAQAEVALNAITSAIAQVASSRGTEGAEINTLSAVE
                     NVQTTQSTNTTSAENDVVATDYASATSNLSKYEILSQTGISALAQANSTQQLVEKLLQ
                     "
     gene            complement(5992..7059)
                     /locus_tag="VFA02_07530"
     CDS             complement(5992..7059)
                     /locus_tag="VFA02_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU09739.1"
                     /translation="MIKKRSRQKSTEKKRLDIRDVARHARVSIATVSRTINKVPTVDK
                     ELAKRVWKAIEELNYFPNTQARALVSGRSRLFGLLISEITNPFFPELIQGFEDVAIEH
                     NYEILIGSTNYDPQRMEICIRRMIERKVEGVAVMTFGIEEPLLDELVSRNIPMVFVDA
                     APPSENVSTLVVDYHHGIREGVQHLAALGHRKLGFISGPMHQRSCQLRRSAFLSSAEE
                     IGVHPPDVWMLEGNHTLEGGMKAMELLLQQKELPTAVMCSNDMTAIGALRVLAKAGLR
                     VPEDISVIGFDDIHLAEFVYPPLTTVRMSRNDLARAAFSALRSHIEPVHAHTKKIWPV
                     PTRLTVRQSTGYPRKNARKGR"
     gene            7246..7938
                     /locus_tag="VFA02_07535"
     CDS             7246..7938
                     /locus_tag="VFA02_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HZU09740.1"
                     /translation="MLDGLREQVLEANLELVRRGLVLYTFGNASGIDRQTGLVAIKPS
                     GVPYEEMKPEHMVITDLTGKIVEGNLKPSSDLATHLLLYREFSTIGAVVHTHSEYATA
                     WAQAGRSIPAYGTTHADYFYGPVPVTEELTAEEINGDYVHNTGLAIVRRFRDLDPDAV
                     PSVLVAGHAPFCWGKTPHDAAHNAVVLEAVARMAYYTATLNPECRGVSQALLDRHYFR
                     KHGPMATYGQKS"
     gene            8083..9702
                     /locus_tag="VFA02_07540"
     CDS             8083..9702
                     /locus_tag="VFA02_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU09741.1"
                     /translation="MVIFIGFYLKGSSNTSEEFFMAGREMTAWIAGLSFVSANLGSLE
                     LMGWAGSAYQYGILATHWYWVGAIPAMLFLGIVMMPFYYISKTHSVPGYLSLRYGEGA
                     RGLSAVCFAFMTVLMSGINMYSMAIVMKVVLGWNLNFSIWVSSITVALYVALGGLRSA
                     IFNEVLQFLLIWLGALLVPILGLVEVGGWSNLRGQILQRLGSSEYVHMWSTLGHFHDN
                     PMGIHWTGIVFGLGLVISFGYWTTDFLVVQRVLSAHNLRAARMSPIIGAAFKMCVPFI
                     VILPGLLALSVLPFHLVGETQAVATGQHSYNEVLPLMLVRYCSPGLLGLGVTALIAGF
                     MSGMAGNVSAFSTVWTYDIYGAFINKRASDQHYVKMGRWCTFIGVLISVGTAYLVQHA
                     ASIMDYVQALFSFFIAPLFGTVIIGMLWKRATKAAGFWGLLAGTVTAIGLWAWVHHDP
                     SALRYVALSADAKAMAEDMYRALWSWLVCVIVTVIVSYMTQPKPEAELEGLVYGASKI
                     PEEHDDHWYQKPIVWASVIFVIFITLNIIFW"
     gene            9719..9931
                     /locus_tag="VFA02_07545"
     CDS             9719..9931
                     /locus_tag="VFA02_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09742.1"
                     /translation="MRETMSIWFFAGVLFLIYGVIIVAEGFWELAHPPLHPPVLFNLH
                     PALWWGGMMAVAGLAYTIKFWPRVRR"
     gene            10053..11450
                     /locus_tag="VFA02_07550"
     CDS             10053..11450
                     /locus_tag="VFA02_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HZU09743.1"
                     /translation="MTFGIIVGSRGFFPHHLAKEGREEMIKVLDAAGYKSIVLSPEQT
                     LHGAVQTPDDARHCAELFKKHREEIDGVIVTLPNFGEERAIVDALRLSTLKVPVLVQA
                     TPDRPDIMKIANRRDSFCGKMSACNNLMQYGIPYSLTALHTVSPSSEAFKKDLAWFAA
                     VCRIVNGFRNLRIGSIGARPAAFNTVRYSEKLLEVNGISVEPIDLSEILGRIDRMRDT
                     DDAAQSKLAAIKAYVSTSGIPDAALMKMAKLGAVIDGWMKETYCTISAIQCWTALEEY
                     FGVVPCTVMSMMSENLFSSACEVDVVGVLSMYGLALASETPSALLDWNNNYGDDPDKA
                     VCFHCSNLPKHFFADVKMDFQEIIAGTVGKENTFGTCVGRVKSGAMTYLRFSTDDFNG
                     KIRGYVGEGEFTNDPLETFGGAGVVRIPKMQKLLRFICEKGFEHHVAANFSTVASPVY
                     EAATRYLGWDMHLHE"
     gene            11539..>13008
                     /locus_tag="VFA02_07555"
     CDS             11539..>13008
                     /locus_tag="VFA02_07555"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HZU09744.1"
                     /translation="MSIVAGVDFGTLSVRVTLLDSEKGRLGTAIAEYPLHRKREDPDY
                     ATQSHKDQMDALVKATRKAIADAKIDGEKIEAMALDTTGSSVIPVGENLEPLDDYYLW
                     CDHRAKEEAQQITELAHKEKLEAIEWCGGVYSHEWGFAKLLHWLRHNPEKRGRFVTAF
                     EHCDMVAGTLCGITDAKKVKRSVCAMGHKWMWNPKWDGLPSQEFLSKLDPLFDGIREK
                     LDGEYATSDKLAGHLSSQWAEALGLRAGIPIPVGAFDAHWDAIGAGCREGDVVNVVGT
                     STCIIAMQKKANLVPGVCGVVPGSVHPAYTGVEAGLSATGDIFEAIARRAGHKVSELS
                     KGLENYRAGQTGLLRLSWDNGDRTVLVNSELGGVTLGWNLVHTAQDELHAAIEGTAFH
                     TRIILERMAEYGVPVDRVINAGGIPQNNQVLNQIYADVLGKPVLVPAGIPTSLGSGIF
                     ALLAAKTFPTVEAAQDKMCLSYKTVEPDPKAHAVYNRLFA"
BASE COUNT         2933 a         3716 c         3520 g         2839 t
ORIGIN      
        1 gaacaggccc aatctgatcc gtcccggcac gcatccccag gcccaacagc agcagcgccc
       61 ggcctctccg cagcgtatcg aagcccgtcc gcaagagcag gaataccgcc ctgctccgca
      121 gcagagacag gaaacgagac ctgcacaacc acagccacgc ccgcagcagg aatatcatcc
      181 tgcgcccgct ccacagccgc gtccaataga tcgcccgcaa ccacaacctc agcacgagga
      241 acaacctcgc ccacaaccac aacgtcagga gccacctcgc ccccagcagg aacaccctca
      301 cgcagaagtg ccgaagccgt cgcctgcacg tgctgaaaac cacccgcagc cgcacacaga
      361 agcaggacac ggaagataac cgaagtttcc atccttcgct tatgtgcagg atggaaacct
      421 cgaatgcacg ttcctcaagc cttcctggcc acgacgatcc tcaacggcgg gaaggtataa
      481 agtgggttcc cccagtgcat ttccgtagtc aaaccggcgc gctgaagctc ctcagcatac
      541 tctttcgtat tcttgtaatc agaaagaatg gccacgcctc caggcttgag tacgcgcgca
      601 atctgctgca cggcgcgcag ccgggccggc ctgtcataaa tgttgtgcaa acagagattg
      661 gaaagcacaa cattgaaact cgcgtctgga aaaggcatct cctgcgccgc aacgcttacc
      721 agttcgcatc ggctgttcac gccttccaat tccagattgc gctgcgtggc tgcctcggaa
      781 tttccggcca tgtcttcctt tgaccagata tctattcccg tagcatgtcc gctgttgagc
      841 ttcttcgccg cccaggcaag cagaagtccg cgtccgcagc ctatatcgag gaccgtctca
      901 tctccgcgcc actgataaag agaaagcatc agatcgcggt ggcggaactt cccatattta
      961 acatagaaaa ggtaaagcag tccctcaagt atcaggaaca atccagtcca gaaaaacatg
     1021 gggcgaagaa gaaagatcac atgcgagatg tgtactgcgc gcgggccaaa aattccaagt
     1081 agcaggcagg gaacgccaaa cagaaataaa ttgcgcagca cgccgggagc atctacaccg
     1141 tagtcgggtt tgtttttcat ttggaagatg atatcttgcg gtgttccatc atctccattg
     1201 ctttcatcaa caggtctctc atgagcgaat cgtggtcgta tccggaagac catgcacgcc
     1261 tgcacccggc agcagcgatc cggtttctcg cttcttcatc cggaagatag cgctgtatct
     1321 ttgcaatgca ctcttccttc gtagaaaaga aaacggcctc ttcatcttcc ttaaacagct
     1381 cgagatggcg cggcgtgcgc tcagccagca taaagccgcc acaggcagta atttcgtaag
     1441 aacgctgcgc cgcctcatcc tgattggatt tggtcacaaa acagaggttg atcttcgatt
     1501 tccagagggc ttcccggtat tccttgcctc ttaacgggcc gttcttaaag acggcctgca
     1561 tcacttccgg cgcaagctct ttttcccacg gcgcgcgcgg cccggaaatg tcgagcggaa
     1621 caccggcttt gaaaagcgac gtaaaccact cggcacgatc gtcatagggc gagccgatga
     1681 aggacacgct tctcgtccgc tcgttgtctg taacaggaga aggcgaaggg aagtgtacat
     1741 cgcgatcaaa gcccaccagc gtcagcacga catccttagc gccatgggcc ttgaacgcat
     1801 caatgcttac tttccgctgc acggcattaa gatcaaactt gggcagcgct ttgcgaaaca
     1861 accggaagcc agggtcctta cgcgggccaa acggattatc aatgaaaaga tcaatcagga
     1921 agacgccctg ctgccgcagt ctgcgaagtg tgcctggagt cagagacaaa ggcttgtccg
     1981 caagcaaaac attggcctta tttctgaccg cggtatcaag aacaaaccgg ttgaacttcc
     2041 acaccttcgg gccgaactga gtacgaaagc gaatcttgtc tacaatgctg gataccggga
     2101 gatgctcatc tgtccggaca acccaggcgc catccaggcc catgcgctgc aaggcattcc
     2161 aacgccccag acttgcagag ccagggtttg tttcagccac aacgtataag acccgcagag
     2221 agctcggtgt cattacaact ccgttgtttt tgtctcgacc agattcgccg cctttgccag
     2281 aaattcaccc agcggcatct gcccctggtc gccctttccg cgggtgcgca cgctcacggc
     2341 acctgcttcg gcttccttgt cacccatcac cagcacatac ggcacctttt gcatcgtaaa
     2401 gtcgcggatt tttgcattca tcttttcgtt acgagcatcc agttccacgc gcagcccggc
     2461 attttgtaac tgctgttgga ttttttctgc atactccaca tgccgttcgc tgatcggcac
     2521 caggcccacc tgcactgggg ccagccagaa tggaaatgct ccggcgtaat gctcaatcaa
     2581 cacaccaaag aaccgctcga ctgacccgaa cagcgcgcga tgtaccatca ccggctggtg
     2641 gcgttcgccg tcctcgccta catattcaag ctcgaacctt gcaggcagat taaagtcaaa
     2701 ctgtacggtc gagagctgcc aaagacgccc cagaacgtcc accagcttta catcaatctt
     2761 cggcccgtaa aatgctgctt cgccggggat ggtcttgaac ggaataccct tgcggtccag
     2821 cgcgttttca agcgaacgga tggccaggtt ccatttctca tctgacccgg cgtagctctg
     2881 cctgtctttc gggtcccagg tggaaagctc aaccttgaaa tcgctgaaac cgaaggtttt
     2941 cagaacagac tgagcaaaat caatacaggc cactacctca tcttcaatct gctctggcgt
     3001 gcagaagata tgtgcatcgt cctgtgtaaa tccacgcacg cgcagcagac cgtgcatcgt
     3061 gccggagcgc tcatagcggt aaacattgcc taactccgcc aggcgcacag gaaggtcgcg
     3121 atagctcttc ggcgaatttt tatagatcag gatgtgcccg gggcagttca ttggcttcag
     3181 ccggtagtcg gcgtcatcca gttccatggg tgtgaacatg ttctgcgcat agaagccttc
     3241 atgcccgctc actttccaaa gctcggtgcg catcacatgc ggggtgtaga ccaaatcgta
     3301 accgcgacgg atacactctt cccgcatcca gtcttccatc accttgcgaa tgatggcgcc
     3361 tttgggatgc cagaagatca gccccgatcc ggccagttcc tggatggaaa acaaatccag
     3421 ttgtttgccc agcacgcgat ggtcgcgttt cgccgcctct tccagccgcg caaaatgctc
     3481 gtccagttct tttctggaga agaaggctgt gccataaatg cgttgcaatt gcgggttttt
     3541 ctcatctccc agccagtacg cgcctgcaag gttcgtcacc ttgaacgcct tcacgcgccc
     3601 cgtcgaagga acatgcggtc cacggcagaa gtccacgaac gccccattgc ggtacagcga
     3661 aatctgctcc cccggtttgg taaagcgttc aataaagtgg accttcatga aatcgttgtc
     3721 cgcccggaag cgctggaggc cttcctcgcg cggctcccat tcgcggacaa acttctcatc
     3781 ccgcgcgacg acgtcggcca tcttctcttc aatcttcttc aggtcttcgg gagtaaacgg
     3841 cgtagggcgg taaaagtcat agaagaagcc tgcatccgtc gccggaccat gtcccagctt
     3901 ggtctcggga aacagctcca gcactgccgt agcgagcacg tgcgccgctg agtgtcgtaa
     3961 cactttgagc gcgtcagggt ccttttccgt caggatttcg agcgccacgt cttcgtggag
     4021 tggggccgag aggtctacca gccgctcgcc atgcgtgttt tccgcgctat acatggcggc
     4081 ctcgccctca gtctcatgca gaaccgccgc tgctgccgta ctcccattgc cattcagtgg
     4141 ccggatacgc gccaccactg ctgcagacgc aagtctgggc gaaatgctct ttgctacgtc
     4201 cagcggagta gaccccgccg gtagttcttt ttgagaaccg tcaggaagct gtacacgaat
     4261 agaggtaaca ggcgaactca tgggtaaata gataggtttc agtcttaaat ccatgctatc
     4321 aggcatggca gggtgcttcc gggtgaggag gggcatagtg cgctatgaaa attgtcatcc
     4381 agatcctgag ggcggtgaag gacagaacct gcaggagctg aggatccggt tcaacgcacg
     4441 gttatagcgc acgttaaatg ccaaacacca aacactccat aaagggacct ttcccttcgc
     4501 cgatgaaggc tatgtgtaac gtggttcctg caggaacagg tgacccggcc ctcaaccggg
     4561 caggtacccg gagcaggcaa tccaatccac acagaagagg agcacaacat atcatgtcat
     4621 tgggtgtcct gaataacatc tccgcgctct acgcggcgaa caacctgaac cagacacagg
     4681 caagtctgca gaacgtgctg acacagcttt cgtctggctc gcgcatcaac tccggcgcgg
     4741 acgacgccgc cggactctca ctcgccaatg gtctgactgc ttctgccacg gcactgtcgc
     4801 agtcagcgaa aaacgcgtct gaaggcgtgg actttctgca ggtggcagat ggcgcgcttt
     4861 cgcaggtcac caacctgttg aaccgtgcgg tcacgctggc cacggaagct tccaacggca
     4921 cgttaaatgc cgcgcagcag gcagccacgc aggctgagta ttcgagtatt atgaccgaaa
     4981 tttcgaccat caactcgaac acggaataca atggcatcaa cgtcttcagc aatgctgcca
     5041 atatttatac gtcggatggt acggtcaatc agtcctatgc cacttctgcg cttggcagca
     5101 cgagttcaga ttcgttgaag ctgggttccg tggtttacgt gagcggaaac gtggcggcgg
     5161 ccaccacaac gcaggctgga aagactgaca gctacggcaa tgccatcaaa gcagctgata
     5221 atcttctgac ggcaacagac cccagtggac acaatgtcgt cattgatctg acggcaatgg
     5281 gcgacaccac cacggctgcc ggcgctcttt cagcggccac cgcagtagcc atttccggag
     5341 gtaaggcgct tgctggaggg gtagctgcgg ccaccgcagc cggaacagat gaatacggtt
     5401 cggcttacac cacggccaat caggtattca catccggtgc gacggtcatc aacatgggta
     5461 caactgctcc aacttccaac gagtatgttg gcacctctct gagcaacaat gcgcaggccg
     5521 aagtcgccct caatgcgatt acttctgcga ttgcacaggt tgcatccagc cgcggcaccg
     5581 aaggtgctga aattaacaca ctgtctgcag ttgagaatgt gcagactacc cagtccacca
     5641 acacaacttc agcagagaac gacgtcgttg ccacagatta cgcgtccgcg acatccaacc
     5701 tgtcgaagta tgaaattctg agccagaccg gcatcagcgc cctggcacag gccaacagca
     5761 cacagcaact agttgagaaa ctgctccagt aatttatggt tgtgggacag cgagagagaa
     5821 ttgagtattt ctcgctgtcc tttttaccgc tgtgcaagcg cacaagttat aaaagcgggc
     5881 acaagattat acaactacac atgaagtcca cttccgcgct atttaaaatg gcagggaaat
     5941 tttttcttac ttactacttt cagtacgccc ctttcatcca ggcaccgaac cctaccgccc
     6001 ttttcgtgca ttctttcgcg gatagcccgt agattgccgc accgtgagcc tggtgggaac
     6061 aggccatatt tttttggtgt gagcatggac tggctcgatg tgtgaacgca gggccgagaa
     6121 ggcagcacgc gccagatcgt tgcgcgacat acgtaccgta gtcagcggag gatagacgaa
     6181 ctcagccagg tgaatgtcgt caaagccgat gacggagata tcttccggga cgcgcagtcc
     6241 tgccttggcc agcacccgca gtgcaccaat cgccgtcata tcattcgagc acatcaccgc
     6301 agtaggaagc tctttctgct gcagaagcag ttccatcgcc ttcatgccgc cttccaatgt
     6361 gtgattgccc tcgagcatcc acacatccgg aggatgcacg ccaatctctt ctgccgaact
     6421 caaaaacgcg ctgcgacgca actggcaaga gcgctgatgc atagggccgc tgatgaagcc
     6481 cagcttgcgg tgccccagcg ccgcaaggtg ttgcacacct tcgcggatac cgtgatgata
     6541 gtccacaaca agtgtgctga cgttttcgct tggtggggcc gcgtccacaa agaccatcgg
     6601 aatattgcgc gataccagct cgtcgagcag aggctcctcg atgccaaagg tcatcacggc
     6661 aacgccttcc actttacgct caatcatgcg ccggatgcag atttccatcc gctgcggatc
     6721 gtagtttgtc gaaccaatca ggatttcgta gttgtgctca atggccacgt cttcaaagcc
     6781 ctggatcagc tccggaaaga acgggttcgt gatttccgaa attaaaaggc caaacaggcg
     6841 gctgcgaccg gagaccaacg cgcgcgcctg tgtgttagga aaatagttca gttcctcaat
     6901 tgccttccag acgcgcttgg ccagttcctt gtccacagtt ggaactttgt tgatggtgcg
     6961 tgatacagtc gcaatagaga cacgcgcatg acgggccacg tcgcgaatgt cgagccgctt
     7021 cttttccgtg gatttctggc gagatcgttt ctttatcatg cttgcaggcg atttttactt
     7081 gacgtggcaa gtgtaaattg cgtaacttca aaaagctagt aaacgtttgc tgaaaaatta
     7141 gtttagcggg aaaaattttt catagaagac gaaacataaa tcggtttagt aatatggccg
     7201 tgtctgaatt tttcccgcag tcctggattg agaggtcttt cttttatgct tgatggtctt
     7261 cgtgagcagg tcctggaggc caatcttgaa ctggtccgcc gtggactcgt tctttatacg
     7321 tttggaaatg ccagcggcat tgaccgccaa accggactgg tcgccattaa gcccagcggc
     7381 gtgccctacg aagagatgaa gcccgaacat atggtcatca ccgacctgac cgggaagatt
     7441 gtagaaggca atctgaagcc gtcctctgac ctggcaacac atcttctgct ttaccgcgag
     7501 ttttccacca tcggtgctgt cgtccacacg cactctgaat acgctacggc atgggcgcag
     7561 gcgggccgca gtatccctgc ttatggaacc acgcacgcgg actattttta cgggccggtt
     7621 ccggtgaccg aagagcttac cgccgaggag atcaacggcg actacgtcca caataccggg
     7681 ctggccatcg tccggcgctt ccgcgatctg gatccggatg cggtgccatc tgttctggtt
     7741 gccggacacg cgcccttctg ctggggcaag acgccacatg acgctgcgca caacgctgta
     7801 gtgctggaag ccgtggcgcg catggcgtat tacacggcca cattgaatcc tgagtgcagg
     7861 ggagtttcgc aggcgctgct ggaccggcac tatttccgca aacacggccc tatggccaca
     7921 tacggacaaa aaagttaatc aactaacgag gcacagccag acatgccctt tttcgccagt
     7981 aaattcttca cccccggcgc actcgccctt ttccagacgc cgggaaaatc tctcgcccac
     8041 ctgaacagca tagacatcct cgtcattgct gtttattttg ctatggtcat cttcatcggc
     8101 ttctacctga aaggctcatc caataccagc gaagagttct ttatggccgg acgggagatg
     8161 acggcctgga ttgcgggcct gagcttcgtt tctgccaatc ttggttcact tgagctgatg
     8221 ggatgggcgg gatcggcata tcaatacggg attctggcga cacactggta ctgggtcggc
     8281 gccattcccg cgatgctctt cctgggcatc gtcatgatgc cgttctatta catctcaaag
     8341 acacactctg tacccggata tctctcgctg cgctatggcg agggcgcccg cgggctttcg
     8401 gccgtgtgct tcgccttcat gacggtgctg atgagcggca tcaacatgta ctcgatggcg
     8461 attgtgatga aggttgtcct cgggtggaac ctgaacttca gcatctgggt ttcgtccatc
     8521 accgtcgccc tttatgtcgc gctcggtgga ctgcgctcgg ccatcttcaa tgaagtcctg
     8581 caattcctgc tgatctggct tggcgcgctg ttggtgccga ttctgggact cgtcgaggtc
     8641 ggcggatgga gcaatctgcg cgggcagatt cttcagcgcc tcggctcttc tgagtatgtc
     8701 catatgtgga gcacgctcgg ccatttccac gacaatccca tgggtattca ctggacgggc
     8761 atcgtctttg gtctggggct ggtcatcagt ttcggatact ggacgacgga cttccttgta
     8821 gtgcagcgcg tgctgtcggc gcataatctg cgcgcggcac gcatgtcgcc gatcatcggc
     8881 gcggccttca agatgtgcgt gccgtttatc gtgattctgc ccggactgct cgccctctct
     8941 gttttgccgt tccacctggt gggagaaaca caggccgttg ccaccggaca acacagctat
     9001 aacgaagtgc ttccgctgat gctggtgcgg tattgcagcc ccgggctgtt aggcctgggc
     9061 gtgactgcgc tgattgcggg cttcatgtcc ggaatggccg gcaacgtcag cgccttttcg
     9121 accgtctgga cttatgacat ttacggcgca tttatcaata agcgcgcctc cgatcagcat
     9181 tatgtaaaga tgggacgctg gtgcacgttc atcggcgtat tgatttcagt aggcaccgcg
     9241 tacctggtac agcacgcggc cagcatcatg gattacgtgc aggcgctctt cagcttcttt
     9301 attgcgccac tgttcggcac cgtcattatc ggaatgctgt ggaagcgggc cacgaaagct
     9361 gccgggttct gggggctgct ggctgggaca gtcactgcca tcggactctg ggcctgggtg
     9421 catcatgatc cttcggcatt gcgttacgtt gcgctttcgg ctgacgccaa ggccatggcc
     9481 gaagatatgt atcgcgcgct gtggtcgtgg ctggtgtgcg tgattgtgac ggtgatcgtc
     9541 agctacatga cgcaacccaa gcccgaggcc gaactggaag ggctggttta tggcgcatcg
     9601 aaaattcccg aagagcacga tgatcactgg taccagaagc cgattgtctg ggccagcgtg
     9661 attttcgtca tcttcattac tctgaacatc attttctggt aaaggagaaa gaggagagat
     9721 gcgcgaaaca atgtccatat ggttttttgc cggggtcctc tttctgattt acggcgtgat
     9781 tattgtggcc gaaggattct gggagctggc acatccgcca ctgcatccgc cagtattgtt
     9841 taatctccat cctgcactgt ggtggggcgg catgatggcc gtcgcgggcc tggcttacac
     9901 gatcaagttc tggccgcggg tcagaaggta ggaattttct caggtcttgt tgagacctgg
     9961 gcacccggat atcacggatg gttgtgccac atctcgaaga gatgtgggca gacaaaggga
    10021 attcaaaggg aacccacaag atgaaaaaac agatgacgtt tggaattatt gttggcagcc
    10081 gcggattctt tccgcaccac cttgccaaag aaggccgcga agagatgatc aaggtgctcg
    10141 acgcggcagg ctacaagagc attgtgctct cgccagagca gacgctgcac ggcgccgtgc
    10201 agacaccgga tgacgcccgc cattgcgccg agcttttcaa aaagcaccgt gaagaaattg
    10261 atggtgtcat cgtcacgctg ccgaacttcg gcgaagaacg cgccattgtg gatgctctgc
    10321 gtctttctac tctgaaagta cctgttctgg tgcaggccac gcccgaccgt ccggacatta
    10381 tgaagatcgc caaccggcgc gacagcttct gcggaaagat gtcggcctgt aacaacctga
    10441 tgcagtatgg tattccgtac tcgctgacgg cgctgcacac agtttcgcca agttctgagg
    10501 ccttcaagaa agacctcgcc tggtttgcgg ctgtctgccg cattgtgaac gggttccgca
    10561 atctgcgcat tggctcgatt ggggcgcgtc cggcagcgtt taacactgtc cgttacagtg
    10621 agaagctgct tgaagtgaac ggcatctcgg ttgagcctat tgatttgtct gaaattctgg
    10681 gccgcattga ccgtatgagg gacacggacg atgccgcgca atccaaactc gctgccatca
    10741 aagcctatgt ttcgacgagt ggcatccctg acgcggccct gatgaagatg gccaagctgg
    10801 gcgcagtgat tgacggctgg atgaaggaga cctactgcac catcagcgct atccagtgct
    10861 ggaccgcgct tgaagaatac ttcggtgtgg tgccttgcac cgtgatgagt atgatgagcg
    10921 agaacctatt ttcaagcgcc tgcgaagtgg acgtggtcgg cgtgctcagc atgtacggtc
    10981 ttgcgctggc atcggaaact ccgagcgcac tgctggactg gaacaataac tacggcgatg
    11041 atccggacaa ggccgtctgc ttccactgct cgaacctgcc gaagcacttc tttgccgatg
    11101 taaaaatgga tttccaggaa atcatcgccg ggactgtcgg caaggagaac accttcggca
    11161 cctgtgtggg ccgcgtgaaa tcgggtgcaa tgacatatct gcgcttctct acagacgact
    11221 tcaatggcaa gatccgcggc tacgtcggcg aaggcgaatt cacgaacgat ccgctggaga
    11281 cattcggcgg agcaggcgtc gtgcgtattc ctaaaatgca gaagctgctg cgctttattt
    11341 gcgagaaggg cttcgagcat cacgttgccg cgaacttttc cactgttgca agcccggtgt
    11401 atgaagcagc cacgcgctac ctgggatggg acatgcactt gcatgaatag gtgctagttg
    11461 cacttgttcg ttgcctgtct ccagttctta ccgacactgg agactgtaaa ctgtgggact
    11521 gtaaaacaag gaaaacacat gtccatcgtt gcaggcgtag atttcggaac actcagcgtg
    11581 cgtgtcacgc tgctcgacag cgaaaagggc cgtcttggca cggccattgc ggaatacccg
    11641 ctccaccgca agcgcgaaga tcccgactac gcaacgcagt cccataagga ccagatggat
    11701 gcgctggtaa aagcgacgcg caaggccatc gccgatgcaa aaattgacgg cgaaaaaatc
    11761 gaagcgatgg cgctcgacac aacggggtca agcgtcatcc ctgtagggga aaatcttgag
    11821 ccgctcgatg attattacct ctggtgcgac caccgcgcca aggaggaagc gcagcagatc
    11881 accgaacttg cgcacaaaga aaagcttgaa gccatcgagt ggtgcggcgg tgtgtattcg
    11941 cacgagtggg gcttcgccaa actgctgcac tggctgcggc acaatccgga aaagcgcggc
    12001 cgttttgtga ccgcctttga gcactgcgac atggttgccg gaacgctctg cggtatcact
    12061 gacgcaaaga aagtaaagcg tagtgtgtgt gccatgggcc acaagtggat gtggaaccca
    12121 aagtgggacg gcctgccttc gcaggaattt ttatcgaagc tcgatccact ctttgatggc
    12181 atacgcgaaa agctggatgg tgagtacgct acatcagaca aacttgccgg ccatctctcg
    12241 tcccaatggg ccgaagccct cggcctccgc gcgggaatcc ctattccggt cggcgcattc
    12301 gacgcgcact gggacgccat cggcgcaggc tgtcgcgagg gcgatgtggt caacgtcgtc
    12361 ggcacatcaa cctgcattat cgccatgcag aagaaggcca atcttgttcc cggcgtctgc
    12421 ggagttgtcc ctggcagtgt gcatcctgca tataccggag tagaagcagg gctctccgct
    12481 acgggagaca tctttgaggc catcgcccgg cgggccggac acaaagtctc tgagttatcg
    12541 aaagggctgg agaactaccg cgccggacag actggactcc tacggctgag ttgggacaat
    12601 ggcgaccgaa cggtgctggt caactccgaa ctgggcggag tcacgctggg atggaacctc
    12661 gtgcatacgg cacaggacga actacacgcc gccatcgaag gcaccgcctt ccacacgcgc
    12721 atcattctcg aacgcatggc cgaatacgga gttccggtag accgtgtgat taacgccggg
    12781 ggcattccgc agaacaacca ggtgctcaac cagatttatg cagatgttct gggcaagcct
    12841 gtgctcgttc ctgcgggcat tccaacgagc ctcggctccg gtatcttcgc actgctggcg
    12901 gcaaagacat ttccaaccgt cgaagctgcc caggacaaga tgtgcctctc ctataaaaca
    12961 gtcgagccgg acccgaaagc gcacgctgtt tataaccggc tcttcgct
//
LOCUS       DASRBV010000195         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_162129, whole genome shotgun sequence.
ACCESSION   DASRBV010000195 DASRBV010000000
VERSION     DASRBV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162129"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1138)
                     /locus_tag="VFA02_07560"
     CDS             complement(410..1138)
                     /locus_tag="VFA02_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZU09745.1"
                     /translation="MLASLLMATHGLIYAQEELPEGPSTAQLASSASVPDSPHPADAD
                     GIRPAVPASPVPPAQEIAIEGFVSYGHWQIFAAGHSEFLYTAGMEYDRNSWGNFLGAQ
                     MDYAAEVLPFVLLREPRVLDRWGNQKSKDYVTLHGLGFSPIGLRMLWRPDKAFKPYFT
                     VKGGAVAFTQKALSPDASYYAFSLSETIGFYTRLSDRWDMRVGLFGDYHFSNAYITSY
                     NPGLDVMNSSIAFSYHLGRSRTTR"
     gene            complement(1381..3636)
                     /locus_tag="VFA02_07565"
     CDS             complement(1381..3636)
                     /locus_tag="VFA02_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09746.1"
                     /translation="MRFFPRSGQFLSLAVVSVFFCGLCVPPGRAQDSTSNQQMGTSQS
                     DKQQDYEPSLPLTLPPGVRQPKPFGQIESTIEVRSDDLINQISPVREGTREQVQSSAG
                     TFQDFSRYLLLMPGTVANSDSFNDLMVRGGHPIENLYVIDGLEVPNINHFAVGGTTSG
                     FTPMINTSTISKVTLQPGVYDSKYWSRLSSVVEIQTRDQDEAVRAGEINLGISGVGGF
                     WENPLGSRVSMLLAADRSLLNLATNDAGLDGVPVYTNGMARFEWSPDKKDTVSFLGLT
                     GGDSIIVKPCAGDGYETLTIDTQYDGLQSTDGMVWQHMHGPTTLSKVTASFSMQKRDI
                     GQQTQLVNGTYPKGTGPGSCTPPTTTPIYQEATFDKLTTIGYNLQHDIHHWLLSAGTT
                     GQLVQLDYNVNQPQGTQSPFNPDPNWTDAVSFHQTPSAWQTGSYAELTGSAGKRWTGT
                     AGVRIETFGLTSAHAVEPRAGLGFRLTDHQGVYASYRHSAQLAPYLDLLSYPQNRNLP
                     PIEADQYSVGADLWRTDMLTVSLEGYRKNYTKEPVSTEYPSLMLANMIYTPGEQFVWL
                     PLKTGGTGHATGLEFFLRGHLTDRMAGIASVTYSRTLYRAADGILRPGNYDFPLVANA
                     MLTFQLPRAYTLSVRNSYTSGRPYTPYNIPLSMQQDRGIYDLSLVNGKRGPAYDRADI
                     SVDRNFYTHSGVVNLYVGLQNILDRKNFLGYVWLPRCEKIKKCVAKIGVPMKEINQMP
                     AFPVAGVRWDF"
BASE COUNT         1041 a         1153 c         1136 g         1006 t
ORIGIN      
        1 ctgatgacct gcggaaactc ccagcgccat tgcgtatcga ggtgcgcata gggcacgacg
       61 taaagtgtcg gttctttggt gatatcaggt gcctgcatgg tctgtgcaac gccgctgatg
      121 gcaatggcag aggcggcgat ggcagagaga gcacttttgg agaaacgagg catgagcagt
      181 ctccggaatt cgcaaggatg agatgatgta aagctggaga gcgcagattg gaatcgttac
      241 cactccagac ggcactcagg ttactgacgg taacgccgca ttgtcaagtg aactgtcccc
      301 ctgactacag accggggggc cgtctgcaag gattcctcat tcaagaactg aggcgggtct
      361 cctcccttcg gcccacaggc cggccgaagg gggaagaccc tgctgcgaat catcttgtag
      421 tgcgcgatct tcccaggtgg taactgaatg caatgctcga gttcatgaca tccaggccgg
      481 gattgtagct cgtaatgtaa gcattggaaa aatgataatc accgaaaaga ccgacgcgca
      541 tgtcccaacg gtctgataat cgcgtgtaaa agccgatggt ttcagacaag ctgaaagcat
      601 aataacttgc gtcaggagac aatgccttct gggtaaaagc cacggcgccg cctttcacag
      661 tgaagtaagg tttgaaggct ttgtccggac gccacaacat tcgcaatcca attggtgaaa
      721 agcccaggcc atgcagagtc acgtagtctt tgctcttctg atttccccat ctatcgagaa
      781 cgcgaggctc tctgagcaga acaaagggca ggacctccgc ggcatagtcc atctgtgctc
      841 ccaggaaatt gccccatgaa ttgcggtcat attccattcc ggcggtgtac aaaaattcgg
      901 aatggccggc agcgaaaatc tgccagtggc cataggaaac aaagccctcg atagcaatct
      961 cctgggccgg ggggacaggg cttgccggaa ctgccggccg gatgccgtct gcatcagccg
     1021 gatgtggtga atcagggaca gaagccgaac tggccagttg agcagtagaa gggccttccg
     1081 gcaactcttc ctgggcgtaa attaaaccat gcgtcgccat aagaagcgag gccaacaaaa
     1141 aagctccaga cactcgagtc ttcaacaagc ggtcacttcc tccgatgggg gaaattgaag
     1201 gcagattcgc taagtttttt atcgtttgca gagcgtatct aatgaatttc tatttacgca
     1261 gataggagcc gggaacaaaa gatccaatgg ttttgagtta tgataactta ctttttctct
     1321 cccgaggaaa aagttatatc tatacacgta catttcacga gaaaacttat tatttttatt
     1381 tcaaaaatcc caacgcactc ccgccaccgg aaaggctggc atctggttta tctccttcat
     1441 aggcacacct atcttggcaa cgcatttctt tatcttttcg cacctgggca accatacata
     1501 tcccagaaag tttttgcggt ctaaaatgtt ctgcagtccg acatataaat tcaccacgcc
     1561 ggaatgggta taaaaattac ggtccactga aatatcagcg cggtcatatg caggaccgcg
     1621 ttttccattt accaggctga ggtcataaat gccacggtcc tgctgcatgg aaagtggaat
     1681 attgtaaggc gtgtaaggac ggcccgaggt atagctattg cgcacggaaa gcgtgtaagc
     1741 gcgcggcaac tggaacgtca gcatggcatt tgcaaccagt gggaaatcat aattgccagg
     1801 ccgcagaatg ccatcggctg cccggtaaag agtgcgtgaa tacgtaaccg aagcgattcc
     1861 tgccatgcgg tccgtcaaat gtccccggag gaaaaactcc agtccggtcg cgtggccggt
     1921 gcctcctgtc ttgagcggca gccagacaaa ctgctctccc ggcgtataaa tcatgttggc
     1981 cagcatcagg ctcggatatt ccgtgcttac cggctccttt gtatagttct tgcgataacc
     2041 ttccaggctt accgtcagca tgtctgtgcg ccacaggtcc gcgcctacgg aatattggtc
     2101 ggcttcgata ggcggcagat tgcggttctg cggataactg agcaggtcca gatacggcgc
     2161 caactgcgcc gaatgacgat aggacgcata cacaccctgg tggtcggtga gcctgaagcc
     2221 gagtccggca cgtggctcaa ctgcatgcgc ggatgtcagc ccaaacgtct cgatgcgaac
     2281 acccgcggtg cccgtccagc gtttgccggc gctccccgtc agctcggcat aactgcctgt
     2341 ctgccatgcg gacggggtct ggtggaagct gactgcgtct gtccagtttg gatcaggatt
     2401 gaacggagac tgcgttccct gcggctggtt tacgttgtaa tccaattgca ccagctgccc
     2461 tgttgttccg gcagaaagca gccagtgatg aatgtcgtgc tgaaggttat agccaatagt
     2521 ggtcagctta tcgaaggtag cttcctgata aatgggggtc gtcgtgggag gcgtgcaaga
     2581 cccggggcct gtccctttcg gatacgttcc attcacaagc tgcgtctgct ggccaatatc
     2641 tcgcttctgc atggaaaaac tcgccgtgac tttggacaag gtggttggtc cgtgcatgtg
     2701 ctgccacacc attccgtctg tggattgcaa cccatcgtac tgggtgtcaa tcgtaagcgt
     2761 ttcataacca tcgcctgcgc agggcttcac gatgatggag tcgccgcctg tgagtccaag
     2821 aaaagaaacc gtgtctttct tgtccggaga ccactcgaaa cgcgccattc cattggtgta
     2881 aaccggaaca ccatccaacc ctgcatcgtt cgtcgccagg ttcaggagac tccggtcagc
     2941 ggcaagcagc atgcttactc gcgagccaag cggattctcc cagaagccgc caacccctga
     3001 aatgcccagg ttgatctctc ccgcgcgcac agcttcgtcc tggtcgcgag tctgtatctc
     3061 gaccacagag gacagccggc tccaatattt ggagtcatac acacctggtt gcagggtgac
     3121 tttgctgata gtactggtgt tgatcatcgg cgtgaatccg ctcgtcgtcc cgccgactgc
     3181 aaagtgattg atgttgggga cttccaaacc atcaatgaca tagagatttt ctatgggatg
     3241 tccgccccgt accatcagat cattgaatga atcggaattg gccaccgttc ccggcatgag
     3301 aaggagatag cggctgaagt cctgaaatgt cccggccgaa gactgcactt gttcgcgggt
     3361 cccttcgcgc actggcgaaa tctggttgat caggtcatct gagcgcactt cgatggtgct
     3421 ctctatctgt ccaaatggct ttggctgccg gacacctgga ggaagcgtca aaggcagcga
     3481 aggttcatag tcctgctgct tgtcagactg ggacgtcccc atctgttggt tcgaagtcga
     3541 gtcctgcgcc cttcccggcg gcacgcaaag tccgcagaaa aacacagaaa ctacggcaag
     3601 agagaggaac tggccgcttc gggggaagaa gcgcatcgta aaccctttct gcacaagcta
     3661 cactggggac tttaaatttc tgtcaactca caatcaatat atgctgctaa gttcctggtt
     3721 acttgctgga taactatctt ttattccctg gcactacccg ttatctttca gagcgaattc
     3781 ataacctaac atgcatagag cgatgtgcaa agagtcattt aaaaaactat cctgaaccta
     3841 acctcagaag atcttgttgg ttcagacaaa acttcagaag ttgttaagtc agtaaatgga
     3901 caaggcagga ggtggatctt gcttctctaa gatactacgg tctgggacaa agttgtccca
     3961 gaccatgatg aatttatttg taaatcagtt ccgaagggcc ctcatggtct caaaatactc
     4021 cgtcacttct tcagaaaatt catcgccctc aggcggaagc ggcataccat gcacaatcag
     4081 cgctttcgcg catccaagcc aatcaccatg aaagtgagca ttgttcaggg gttctcagaa
     4141 agtgcccagc tcgtctccgg cttttgctag tgaaagcatc agcgattcag cactcaccga
     4201 atcgccggac gcctactcag cagtcctgct tcatgcccgg ctgacagcca cctgggtgag
     4261 ttgctcgatc cactcctcgc cgggtttctt cggttctccc atcgcggcat tcgacacgtc
     4321 ctcccacgct tcccat
//
LOCUS       DASRBV010000196         9104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_315682, whole genome shotgun sequence.
ACCESSION   DASRBV010000196 DASRBV010000000
VERSION     DASRBV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315682"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2210)
                     /locus_tag="VFA02_07570"
     CDS             complement(<1..2210)
                     /locus_tag="VFA02_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZU09747.1"
                     /translation="MPENPISRRSFLQGCAAALALTPVLKGLGAKNGRGPAQRVLSLN
                     QDWLFAGKASAPLNESASSRVTLPHCVVPLSWQKWDPSSWEDVWLYHRHFQLPPELRG
                     LRLFLQFDRAMAAARPQINGHPLPQHLGGFLPFEYEITHLVTAQDNLLTLAVDSRWLN
                     VPPSGSPQGPGSIDYLLPGGINGAVSLHAVPAIFIRDVFAKPVDVLSTDRRLEIACEI
                     DCSIPLPATVRIEAALHDGAGTIARASKDADIGKTNQEILLTIDGLRNITLWDVDHPR
                     LYGLTVTLFHENKPLHRYNTRTGFRDARFEADGFFLNGRRLRLFGLDRHELYPYVGFA
                     ASDRALRRDAEILRNSFNCNAVRCSHYPQSGAFLDACDELGLLVWEELPGWQYIGDEA
                     WQQLAMRDVEAMVRRDRNRPSIIIWGVRINESRNDPALYLRTKEIAKSLDGSRPTSGT
                     MTSLSMKDWHQDVFAYDDYHSAPDGSVAIATPLPGVPFMISEAVGQFNYGGKGFGRKY
                     RRAGSPELQMQQPLLHAQAHNRAADHPRCAGVIAWCAFDYASLMNAYDGVKCPGVADV
                     FRIPKLGASFYLSQADPRIRPVIAPGFYWDFGPQTPSGPGEHAVIFSNCDRLELFINN
                     KPFAVLHPDRAGFPNLKYPPFFADLTFSKAEKPELRPELRIDGYIASHLALSRRLSSD
                     PSLDKLWLHADDAELLADGTDTTRLAFGVTDRFGAPRPFVTGTVSFLLDGPGVLV"
     gene            2337..3161
                     /gene="trmD"
                     /locus_tag="VFA02_07575"
     CDS             2337..3161
                     /gene="trmD"
                     /locus_tag="VFA02_07575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZU09748.1"
                     /translation="MQFDIITIFPEFFSSILAHGVLRRGIASGLLSVETHNLRDFAHD
                     RHRTVDDRPFGGGEGMVLKPEPLAEAIESLEIGAKDERNRQKESVVLLSAQGEKFTQA
                     TARELAKLKRVVFICGRYEGVDERVNELFCDRELSIGDYVLSGGELAAAVILDATMRL
                     VPGVLGNEASSAYESFGQPDALFPESGDGPPRSTHGSGGLLDYPHYTRPAEFRGLTVP
                     EVLSSGNHDMIRRWRRERALEKTWRNRPDLLAVADLSEEDREFLEKLKAIRRGVPG"
     gene            3262..3609
                     /gene="rplS"
                     /locus_tag="VFA02_07580"
     CDS             3262..3609
                     /gene="rplS"
                     /locus_tag="VFA02_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZU09749.1"
                     /translation="MHPVMQRLAEKLNRTDLPPFVPGDTVRVQVKIKEGDKERLQAFE
                     GIVIARKNGPQGSFTVRKMSFGQGVERIFPFNSKVIDKVEKVRSSRVRRAKLFYLRGL
                     RGKAARLKEIERA"
     gene            complement(3665..4276)
                     /gene="ispF"
                     /locus_tag="VFA02_07585"
     CDS             complement(3665..4276)
                     /gene="ispF"
                     /locus_tag="VFA02_07585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZU09750.1"
                     /translation="MSMRIGFGWDSHAFKAGVPLKIGGLAIEHPEGLAGHSDGDVLLH
                     AITDALLGAVSAGDIGSFFPPGDPRWKGADSAIFLNLALEEIQNAGYHIVNVDTTLIL
                     AAPKISPIAGELREHVAGLLDVRPNAVSIKAKTPEGLNADHVAQAHAIVLLEKVEDPS
                     ELKSMSEVIESQRQLEDVVRDLVSQVYSAPLERKKTFDTEDIT"
     gene            complement(4423..5169)
                     /locus_tag="VFA02_07590"
     CDS             complement(4423..5169)
                     /locus_tag="VFA02_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU09751.1"
                     /translation="MAEVLRANQKLIAIGRLTGSIAHEINNPLEAIANLLYLIEQDAE
                     LSPQSRSYLKLAEQELGRVVQISKQTLNFYRETSAPVSMLIKDLLEEVLVLYARRLKE
                     KHIHLSRKYKPSQMVSVFPGELRQVFSNLIANAIEACPPDGSLWIRVRPAGMDGDKKG
                     VRITIADNGTGMEPSVLRKIGRPFLTTKGHHGTGLGLWVTMSILARYGARLRVRSTTR
                     PGRHGTVFSILMPAEARPQGNREELWRAAS"
     gene            5569..7071
                     /locus_tag="VFA02_07595"
     CDS             5569..7071
                     /locus_tag="VFA02_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09752.1"
                     /translation="MRHWKRVAGGLLLGCFGVLGIQAQNNSTGPAAGNPYNSALNPYY
                     GSVQAVAYTPEVKQLSLDDAIRLGIENNLALTLARENQKTAEAQTAQTLNVFLPNIDI
                     SGGTGIHQFNLQAQGFRPGLLSSFSALVPASEAAHFPFIVKIDLTQGQANLSQTLFNW
                     AGWDLYRAAKASAKSAYYNAQSSRGLVVLNVGTAYLQVLADQSQLDYAKSLLKADETL
                     LYQAVEEHKAGTAANLDELRARVQYQQQQQAVIAAENALEKDEIALKREIGMDPRQPI
                     QLTEAAPFADLAAMDIEDAKRQAYTSRQDYQSLLQQLRFAELERKAATHERYPTISFN
                     GNYGVNGISGGPYHGTFAAVGTLNIPIFQEAQFRGDRDVAEAQLQNIRSQIADLRNKI
                     DQQLRDSLLDLQSTADLVRVSKNNVDLATTALEQTTERFQAGVDDSLPVVEAQSTLAQ
                     AQARYVDSVRQYNLAKLGLARNLGIIDTQYKAYLQGGTPPEIKSKQTSQP"
     gene            7252..>9104
                     /locus_tag="VFA02_07600"
     CDS             7252..>9104
                     /locus_tag="VFA02_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU09753.1"
                     /translation="MSLTLTDWLVIAGYLLFNLLIGLYYRSKASGSTEDFFVSGRDVS
                     WWLAGTSMVATTFAADTPLLVCGLVATQGISGNWIWWSLCLSGMTTVFFFARYWRRAE
                     ILTDVELVEIRYGGKPAAFLRGFKAIYLGLLMNCFILGWVTRAMVDIIAVVLGPVIAE
                     GRVLQVSIGGHALMHYTLGDPRHTALAICIFILVPFTGLYTFLGGLWGVLVTDLFQFA
                     LKMAMIVVLAWIAVADLGGMHALKVQLSVVDAATRAAGQPTSNVLSFLPDFHAGLVTN
                     HIWTLPLLTFMMYIGVQWWASWYPGAEPGGGGYVAQRMFSAKNEQHSLGATLWFNIAH
                     YALRPWPWIVTGLVALAVYSPHGGLHPSAAFAANPQQGYVMVLRDYLPPALRGLMVAA
                     FLAAYMSTIGTQLNWGTSYLVNDFYRRFWVREGSERHYVFVSKIITVILVLASGYVAS
                     QLTSISAGWELVLNLGAGTGAVYILRWFWWRVNAWSEISAMIVAALVALGLAHVHFTG
                     NDAVVFAKSTLITAGITTVAWIAFTFFTKPEKEETLLAFYRRVLPAAYGWKHIARLAP
                     ELPQVHDFTANAFDTVMGCVLVYGTLFGLGKYVFGDWISGTVLLVLAAIAG"
BASE COUNT         1840 a         2328 c         2904 g         2032 t
ORIGIN      
        1 acgagcacgc ccggtccatc gagcaaaaaa gaaactgttc cggttacaaa tggacggggc
       61 gctccgaatc tgtctgttac gccgaatgcg aggcgggttg tgtctgttcc gtctgccagc
      121 agctctgcgt catcggcatg gagccagagt ttgtccaggg agggatcgga ggaaagcctg
      181 cgcgagagtg cgaggtggct ggcgatgtat ccgtctatac gcagttctgg gcgcagctcc
      241 ggcttttctg ctttgctgaa cgtcaggtca gcaaagaagg gtggatattt caggttggga
      301 aagccggcgc ggtcgggatg cagaacggca aagggtttgt tattgatgaa cagctccaga
      361 cggtcacagt ttgagaagat aacggcgtgt tcgccggggc cggagggcgt ctgtgggcca
      421 aagtcccagt aaaaaccggg tgcaatgaca ggccgtatgc gggggtctgc ctgcgagagg
      481 taaaaggaag cgcctagctt gggaatgcgg aagacatcgg ccacgccggg acatttcact
      541 ccgtcatagg cattcatcag gctggcgtaa tcgaaggcgc accaggcaat gactccagcg
      601 catcgcggat ggtcagcggc ccggttgtgt gcctgcgcat ggagaagggg ctgctgcatc
      661 tgcaactcag gactgcccgc gcggcgatat tttcttccaa agcctttgcc gccgtaattg
      721 aactggccga cggcctcgga gatcatgaag ggcactccgg gcagaggggt cgcaatggcg
      781 acactgccgt ccggagcaga atggtaatca tcgtatgcga acacgtcctg gtgccagtct
      841 ttcatggaga gggatgtcat cgtgcctgac gtggggcggg agccatcgag tgattttgca
      901 atctcttttg tgcgcagata gagcgcgggg tcgttgcggg attcattaat gcgcacgccc
      961 cagatgatga tggaaggacg gttgcggtcg cggcgcacca tcgcttccac atcgcgcatg
     1021 gcgagctgct gccaggcttc gtcgccgatg tactgccagc cgggtagttc ttcccagacc
     1081 agcaggccaa gctcatcgca cgcgtcaaga aatgcgcctg actgtgggta atgcgagcag
     1141 cgcacggcat tgcagttgaa gctgttgcgc agaatttcgg catcacgtcg cagggcgcgg
     1201 tctgacgcgg caaagccaac gtatggataa agttcgtggc ggtccaggcc gaagagccgc
     1261 agacgccggc cattcaggaa gaagccatcg gcttcaaagc gcgcgtcacg aaaccccgtg
     1321 cgcgtgttgt aacggtgcag cggcttgttt tcatgaaaaa gtgtgacggt aaggccgtag
     1381 agccgcggat ggtccacgtc ccataacgtg atgttcctga ggccgtctat cgtcagaaga
     1441 atttcctggt ttgtcttccc gatgtctgcg tctttcgatg cgcgcgcaat ggtgccggcg
     1501 ccgtcgtgca gtgcagcttc gatgcgaacg gtagcgggca gagggatgct gcaatcaatt
     1561 tcgcaggcga tttcgagtcg cctgtctgtg ctgagaacgt ctactggttt ggcgaagaca
     1621 tcgcgaatga agattgccgg aacggcgtgg aggcttactg ctccgttgat gcctccggga
     1681 agcagataat caatagaccc aggcccctgg ggtgagccgg agggcggcac gttgagccag
     1741 cgggaatcta cggccagcgt gagcaggttg tcctgcgcgg tcaccagatg agtgatttcg
     1801 tattcaaatg gcagaaagcc accgagatgt tgaggtaatg gatggccatt gatctgtggc
     1861 cttgcggccg ccatcgcgcg gtcgaattgc aggaagagcc ggagaccgcg aagctccggc
     1921 ggaagctgga aatggcggtg atagagccag acgtcttccc aggaggatgg gtcccacttt
     1981 tgccacgaga gtggaacgac acaatgcgga agagtgacgc gggaagatgc actttcgttg
     2041 agtggcgcgc ttgctttgcc ggcgaagagc cagtcctgat tgagtgaaag gacgcgctga
     2101 gcagggccgc ggccgttttt cgccccaagg cccttcaata cgggtgtcaa ggccagcgcg
     2161 gcggcacatc cctgaagaaa ggagcggcgg gagatggggt tttcgggcaa aaacgttctc
     2221 ctgatggagg aaatggacat attctttata aacgtttaaa gcgctgagtg cgcctgcgat
     2281 ttcaaaagcc ggaaccaggg ccttttcttt cgaaaatcta caataggaac gtccttatgc
     2341 agttcgacat cattacgatc tttccggaat ttttttcgag cattcttgcc catggagtgt
     2401 tgcggcgcgg tattgccagt ggactgcttt cggtggaaac gcacaatctt cgcgacttcg
     2461 cgcatgaccg ccaccggacg gtggacgaca ggcctttcgg cgggggagag ggcatggtcc
     2521 tgaagccgga gccactggca gaggccattg aatcgctgga aattggggca aaagatgagc
     2581 gcaaccggca aaaagagagt gtggtcctgc tatcggcgca gggggagaag tttacgcagg
     2641 ccacggcccg cgagctggcg aagctgaagc gcgttgtgtt tatctgcggg cgctatgaag
     2701 gtgtggatga gcgcgtgaac gagctttttt gtgaccggga gctttccatc ggcgactatg
     2761 tgctctcagg aggagagctg gcggctgcgg tcattcttga tgccaccatg cgcctggtcc
     2821 ctggcgtgct gggcaatgag gcgtcgagcg cttacgagag ctttggtcag ccggatgccc
     2881 tttttccgga aagcggcgat gggcctcccc gatctacgca tggctcgggt ggacttctgg
     2941 attatccgca ctatacccgt ccggcggaat ttcgcgggct gacggtcccg gaagtcctgt
     3001 cgagcgggaa ccatgacatg attcggcgat ggcggcgcga gcgcgcgctg gaaaagacgt
     3061 ggcgcaaccg gcccgatttg ctggcagtgg ctgatttgtc agaggaagac cgggagtttc
     3121 tggagaagtt gaaagcaata cgcaggggcg tcccgggttg aaatgatttg cattttccgt
     3181 ccactccgtg tccgctgcaa atccttctat tttcgtgtaa actgaaagtt ctgacttcaa
     3241 ttgcaggaaa gaaccatgtc tattcatccc gtcatgcagc gccttgccga gaaactgaac
     3301 cgcacggacc tgcctccttt cgtccccggc gacacggtgc gcgtgcaggt caagatcaag
     3361 gaaggcgaca aggaacgcct ccaggcgttt gagggcattg taattgcccg caagaatggc
     3421 cctcagggta gtttcaccgt ccgcaagatg agcttcggcc agggcgtgga gcgtattttc
     3481 cccttcaact cgaaggtgat tgataaggtc gagaaggtcc gttccagccg tgtgcgccgc
     3541 gccaagctgt tctatctccg cggattgcgt ggcaaggccg cccgcctgaa ggaaatcgag
     3601 cgcgcgtaaa cttttaactt tatttctgat ggatgccccg gttttggccg gggcattctc
     3661 atttttacgt aatgtcttct gtatcaaagg tctttttgcg ttcgaggggc gcgctgtaca
     3721 cctgactgac caggtcgcgt acgacgtctt ccagttgccg ctggctttcg atgacttcgc
     3781 tcatgctttt cagctcggat gggtcttcta ctttttccag aagaacaata gcatgggcct
     3841 gggcgacgtg gtcggcgttg aggccctcag gcgtttttgc cttgatgctt acggcgttgg
     3901 gccggacatc cagaagcccg gccacgtgtt cgcgcagttc tccggcgatg ggagagattt
     3961 tcggcgcggc aagaatgagc gttgtatcca cattgacgat gtggtatccg gcgttctgaa
     4021 tctcttcgag ggccagattg agaaagatgg ccgagtcagc gcctttccag cgtggatcgc
     4081 ccggcgggaa gaagctgccg atgtctccgg cagagacggc accgagcagc gcgtcggtaa
     4141 tggcgtgaag cagcacgtcg ccgtccgagt gtccggcaag accttcgggg tgttcgatgg
     4201 cgagtccgcc gatttttaaa ggcacacctg ctttgaaggc gtgtgagtcc cagccaaatc
     4261 caatgcgcat gctcatagtg ttctctcact aatgtactag gtgaagcgcc cgggaagttc
     4321 gtgatgtaag gaaccatagt gccgtctggt gagaaatctt cgcccggccg gccacacctg
     4381 acgtggttgc ttcgattggg atgacagtat tttatggcgg gatcagctgg ctgcacgcca
     4441 tagttcttcg cggttgccct gggggcgcgc ttctgccggc atcaggatgc tgaagacggt
     4501 gccgtggcgg ccgggtctgg ttgtggagcg cacgcgcaag cgagcaccat agcgggcaag
     4561 gatggacatg gtgacccaca ggccgagtcc ggtgccatga tgacctttgg tggtgaggaa
     4621 tggtctgccg atttttctga gcacggaggg ttccatgcca gtgccgttat cggcaatggt
     4681 gatgcgcaca ccctttttgt cgccgtccat gccagcaggg cgcacccgta tccagaggga
     4741 gccgtccggc gggcaggctt caatggcatt ggcaatcagg ttagagaaga cctgacgtaa
     4801 ctcgcccggg aagacggaaa ccatttggga aggcttatat ttgcgggaca agtggatatg
     4861 tttttccttt agcctgcgcg cgtagagtac cagcacttct tcgaggaggt cttttatgag
     4921 catagaaaca ggcgcggagg tttcgcggta aaagttcagt gtctgcttgc tgatctggac
     4981 gacgcggccc agctcctgct cagcgagttt gagatacgac cttgactgcg gagacagttc
     5041 agcatcctgc tcaatcagat aaaggaggtt ggcgatggct tccagtggat tattgatttc
     5101 atgggctatg gacccggtaa gccgcccgat ggcgataagc ttctggttgg cgcggagcac
     5161 ctctgccatt tgggaggcat tttcccgcgg ctttagagga tcgccgttgt cgccgcctct
     5221 gtgttggtat tggttcatat tcaggacccg catgtgctta ctcctgaacc atctgcagag
     5281 atggcgtatc ccaaactgcg gccttttccg gaagtgggcc ccatgtgcgt ccatttgtgc
     5341 tgcctgctgc aaaaccgttt acctgcccat tccgaaaggg ccaaatttct tattgtttcg
     5401 atgtgcgcgg aagaagaggt tgttgcctga atgccacaat ggggaaaccg gaagcccacg
     5461 ttttgcagtg atttaccgtg gttttatgaa catttttggc aacctgggcc gccccgcgta
     5521 atctaaaaga agagccaaga gactgaattc agaggatttt gtatcgtaat gcgccattgg
     5581 aagcgggttg caggggggct gttgctgggg tgttttgggg tgctgggaat ccaggcccaa
     5641 aacaactcga ctggtccggc ggcaggcaat ccgtacaatt cggctttgaa tccttactac
     5701 gggagcgtgc aggctgtagc ctatacgccg gaggtgaagc agctttcgct cgatgacgcc
     5761 attcgcctgg gaattgaaaa caatcttgcg ttgacgctgg cgcgcgaaaa ccagaagacg
     5821 gccgaagcgc agacggccca gacgctcaac gtctttttgc cgaacattga tatcagcggc
     5881 ggcacaggca ttcatcaatt caatcttcag gcgcaaggct ttcgtccggg actgttgtcg
     5941 tcctttagcg cattggtgcc ggcatcggag gcagcgcatt ttcccttcat cgtaaaaatt
     6001 gatctcacgc aagggcaggc caatctttcg cagacactct ttaactgggc gggatgggac
     6061 ctgtaccggg cggcgaaggc cagcgcgaag tcggcctact acaacgcgca gtcgtcgcgc
     6121 gggctggttg ttctgaatgt gggaacggcg tatctacagg tcctggccga ccagtcacag
     6181 cttgattacg cgaagtcttt gctgaaggcg gacgagacgc tgctgtatca ggcggtagag
     6241 gagcacaagg cgggtacggc ggccaatctg gacgagctgc gcgcgcgcgt tcagtatcag
     6301 caacaacaac aggcggtcat cgcggcggag aatgctcttg aaaaagatga gatcgcgttg
     6361 aagcgcgaga tagggatgga tccgaggcag ccgattcaac tgacggaagc ggcgcccttt
     6421 gccgatctgg ctgcgatgga cattgaagat gccaagcggc aggcatacac cagccgccag
     6481 gattatcagt ccctgctcca gcaattgcgc tttgccgaac tggagcgcaa agcggcgacg
     6541 catgagcgct acccgacgat cagctttaat ggaaattatg gggtgaatgg gatttccggc
     6601 ggcccttatc acggaacatt tgctgctgtc gggacactga atattcccat ctttcaggaa
     6661 gcgcaatttc gcggcgaccg cgatgtggcg gaagcacagt tgcagaacat ccggtcacag
     6721 attgccgatc tccggaacaa gattgatcag caattgaggg acagcctgct tgatctgcaa
     6781 tccaccgccg acctggtgcg cgtatcgaag aacaatgttg atcttgcgac gacggccctg
     6841 gagcagacca cggaacgctt tcaggcaggc gtggatgaca gtcttcccgt ggtggaagcg
     6901 cagtctacgc tggcgcaggc gcaggcgcgg tatgtggaca gcgtaaggca gtacaatctg
     6961 gcgaagctgg ggctggcgcg caacctggga atcattgaca cgcagtacaa ggcctatctg
     7021 cagggcggaa ctccacctga gatcaagtca aagcagacaa gccagcctta ggaactctgc
     7081 gggccaccag cacgcgaaca ccgcgcacac ctgaagcatc caccgcgcca tttccaaaaa
     7141 gcagcatggc gaaggaatag actgagactc gcgtggaaag ttgtttcctg aaggctgtaa
     7201 acctgtatat actgctttca cacccagaat tcctgcacag agagaaacgc aatgtcgctt
     7261 accctgacag actggcttgt gattgccgga tatctgcttt ttaatctgct gattggtctt
     7321 tactatcgca gcaaggccag cggctcgacg gaagattttt ttgtttcggg gcgcgacgtc
     7381 tcctggtggc tcgccgggac atcaatggtg gcgacgacat ttgctgcaga cacacccttg
     7441 ctggtgtgcg gtctggtggc gacgcaaggc atttccggca actggatatg gtggagtctc
     7501 tgcctgagcg gtatgacgac ggtgtttttc tttgcgcgat actggcggcg cgctgaaatc
     7561 ctcactgacg tggagctggt ggagatacgc tacggcggca aacctgctgc atttctgcgc
     7621 ggattcaaag ccatctatct tggcctgttg atgaactgct tcattctggg atgggtgacg
     7681 cgggcgatgg tggacatcat cgccgtggtg cttgggccgg tcatcgcaga ggggcgagtg
     7741 ctacaggtga gtatcggcgg acacgcgctg atgcactaca ctctgggcga tccgcggcac
     7801 acggcgctcg ccatttgcat tttcattctg gtgccgttca ccgggctgta tacgtttctg
     7861 ggcgggctgt ggggcgtgct ggtgaccgac ctgtttcagt tcgcgctcaa gatggcaatg
     7921 attgtggtgc tggcgtggat tgcagtcgca gacctcggcg ggatgcatgc tctgaaagtg
     7981 cagctctcgg ttgtggatgc ggcgacgcgc gcggcggggc agcctacatc gaatgtgctg
     8041 tcgttcctgc cggatttcca tgccggactg gtcacaaacc atatctggac gctgccattg
     8101 ctgaccttca tgatgtatat cggcgtacag tggtgggcct cgtggtatcc gggcgcggag
     8161 ccgggaggcg gcggatatgt agcacagcgc atgttcagcg caaaaaacga gcagcattcg
     8221 ctgggcgcga cattgtggtt taacatcgcg cattacgcgc tgcgtccgtg gccgtggatt
     8281 gtgactgggc tggtggctct ggcggtctat tcaccgcatg ggggactgca cccgagcgct
     8341 gcgtttgcgg ccaatccaca gcagggatac gtgatggtgc tgcgcgatta tctgcctccg
     8401 gcgctgcgcg ggctcatggt ggcggcgttt ctggcggcgt atatgtccac gattggtacg
     8461 cagctcaatt ggggcacttc gtatctggtg aatgattttt accgccgctt ctgggtgcgg
     8521 gaggggagcg agcggcatta cgttttcgtc agcaaaatca ttacggtgat tctggtgctg
     8581 gccagcggat atgttgccag ccagcttact tccatcagcg cggggtggga actggtgctg
     8641 aatctgggcg caggcacagg ggcggtctac atcctgcgct ggttctggtg gcgcgtgaat
     8701 gcgtggagcg agatttccgc gatgattgta gctgcgctgg tggcgttggg gctggcgcat
     8761 gtccacttca ccggaaacga tgccgtggtc ttcgccaagt ccacgctgat tactgcggga
     8821 attacaactg tggcgtggat tgcctttaca tttttcacca agccggagaa ggaggagacg
     8881 ctgctggcct tctaccgccg cgttctgccg gccgcctatg gctggaagca tattgcccgg
     8941 cttgctccgg agctgccgca ggtacacgac tttactgcga atgcgtttga taccgtaatg
     9001 ggttgcgtgc tggtgtacgg aacgctcttc gggctgggca agtatgtttt cggagactgg
     9061 atttccggga cagtgctgct tgttcttgca gcgattgcgg gata
//
LOCUS       DASRBV010000197         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_520199, whole genome shotgun sequence.
ACCESSION   DASRBV010000197 DASRBV010000000
VERSION     DASRBV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520199"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..892
                     /locus_tag="VFA02_07605"
     CDS             92..892
                     /locus_tag="VFA02_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09754.1"
                     /translation="MALVAVEHLSKSYPRRKGLRAEQVLVVDDVSFTIRRGETLGLVG
                     ESGSGKSTIARMVLGLIQPTSGRVVFDGQPITGASQRSLHPIRRRMQPVFQDPFAALN
                     PRMKVAEIITEPLVIHRIVPKKELRAKAAELLRSVGFDESALNRWPHEFSGGQRQRIN
                     IARALALRPELLVLDEPVSALDVSVGAQIVNLLRDLQREFQLTYLFISHSMPLVRYLS
                     TNIAVLHRGKLVEYGPRDVICEAPQQEYTRTLIASTPEIRVSPGAPHS"
     gene            955..1212
                     /locus_tag="VFA02_07610"
     CDS             955..1212
                     /locus_tag="VFA02_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZU09755.1"
                     /translation="MTAPVNTIGLIAAFSTTTAFVPQLLRVWRLKSARDISLHMFLLF
                     SFGEVMWLVYGLLIRSTPVILANVVTLALALAILALKLRYE"
     gene            1233..1670
                     /locus_tag="VFA02_07615"
     CDS             1233..1670
                     /locus_tag="VFA02_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZU09756.1"
                     /translation="MGHEGIRLVSEEEARRAEGAARGLSPEAKTPAKVRVFKTEGTGM
                     EILWKDGHHSHWSFAWLRNACPCATCHDEREKSGRKPGEPKPKTQSLLPMYQAPPRPE
                     TVTPVGHYAISFHWNDGHQSGIYSWDYLRRHCECAECAELRVP"
     gene            1689..>2114
                     /locus_tag="VFA02_07620"
     CDS             1689..>2114
                     /locus_tag="VFA02_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU09757.1"
                     /translation="MNPVTARLSPRASFERTLASAKRTMVLSEIVRLAIDSFRASKVR
                     FALTALGMVIGSASLILVVTIGLTGKQYILNLLQSIGTNMVELEYSGGGTGSINNFQS
                     DYLTRDDERAVREQVPSVMYSSPMLEMHDRISFGQGRVKD"
BASE COUNT          432 a          607 c          630 g          445 t
ORIGIN      
        1 attgggttgc agattctggg caggcacttc catgaagcca tcgcgctgcg ggtggcgcag
       61 gcggtcgagt ctctgcaaag cgaatcgtaa cgtggccctt gtcgctgtcg agcacctgtc
      121 gaaaagctac ccgcgccgta aaggtctgcg ggcagagcag gtactcgtgg tggatgatgt
      181 ctccttcacc atcaggcgcg gagaaacact ggggcttgtt ggcgagtccg gctccggtaa
      241 gagcaccatt gcccgcatgg tgctggggct gattcaaccc acatcagggc gcgtggtctt
      301 tgacggccag cccatcaccg gagcatcgca gcgcagcctg caccccattc ggcgccggat
      361 gcagcccgtc tttcaggacc cctttgccgc gctcaatccg cgcatgaagg tggcagaaat
      421 catcactgag ccgctggtga ttcaccgcat agtcccgaaa aaagagctac gtgcgaaagc
      481 ggcggaattg ctgcgctcgg tgggattcga tgagtccgcg ctcaaccgct ggccgcacga
      541 gttttccgga ggacagcggc agcgcatcaa cattgcgcgt gccctggcgc tgcggcccga
      601 attgctggta ctcgatgagc ctgtttcggc gctggatgtg agcgtgggcg cgcagattgt
      661 gaatcttctg cgcgacctgc agcgcgagtt ccagttgacg tatctgttca tctcgcattc
      721 gatgccgctg gtgcgctatc tctcaacgaa cattgccgtg ctgcaccgcg gaaagctggt
      781 cgaatacggg ccgcgcgatg tcatctgcga agctccgcag caggaataca cacggacgct
      841 gattgcctct acgcccgaga tacgggtttc tccgggtgcc ccacattcct gaagggatgt
      901 gggatccgag ttgtttcagc tcttctctga gaaaatggaa agcagaacat ttccatgacc
      961 gctccggtaa acacgattgg actcatcgct gccttctcca ctacaactgc ctttgttccg
     1021 cagcttctgc gcgtgtggcg attgaagagc gcgcgggaca tctcgcttca tatgttcctg
     1081 ctcttctcgt ttggtgaagt catgtggctc gtgtatggac tgctcatccg ttccacgcct
     1141 gtgattctgg ccaatgtggt tacactggcg ctggcgctgg caatcctggc cctcaagctg
     1201 cgctatgaat aagcgggcag tacactaaag atatggggca cgaaggcatc cggttggttt
     1261 cagaagaaga ggcgcgcagg gccgaagggg cagcgcgtgg gctttctccg gaagcaaaga
     1321 ccccggcgaa ggtgcgcgtt ttcaagactg aaggcaccgg catggagatc ctctggaagg
     1381 acggccatca cagccactgg agttttgcct ggctgcgcaa tgcctgtccc tgcgccacct
     1441 gtcacgacga gcgcgaaaaa tccgggcgca aaccgggaga accaaagccg aaaacacaat
     1501 cgctgctgcc tatgtatcag gcgcctcccc ggcctgaaac ggtcactccc gtaggacact
     1561 acgccatcag cttccactgg aacgacggtc accagagcgg gatttattcc tgggactacc
     1621 tgcgcagaca ttgcgaatgt gctgagtgcg cggaattgcg cgtaccataa ataagttgga
     1681 ctgacgttat gaatccagtc actgcgcgcc tttcaccgag agcgtcgttt gaaagaacgc
     1741 tggccagcgc caagcggacc atggtgctga gcgagatcgt gcgtctggcc attgacagct
     1801 ttcgcgccag caaggtgcgt tttgcgctca ccgcgctggg catggtgatt ggctccgcat
     1861 cgctcatcct tgtggtcacc atcggactca ccgggaagca gtacatcctg aacctgctgc
     1921 aaagtatcgg cactaacatg gtcgagctgg aatactccgg cggcggcacc ggatctatca
     1981 ataatttcca aagcgactat ctcacgcgtg atgatgagcg cgccgtaaga gaacaggtgc
     2041 cgtccgtcat gtattcttcg cccatgctgg agatgcatga ccgcatcagc tttgggcagg
     2101 gccgggtgaa agat
//
LOCUS       DASRBV010000198         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_469211, whole genome shotgun sequence.
ACCESSION   DASRBV010000198 DASRBV010000000
VERSION     DASRBV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469211"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..568
                     /locus_tag="VFA02_07625"
     CDS             143..568
                     /locus_tag="VFA02_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HZU09758.1"
                     /translation="MYPEIMVVPMREELTRVGIKETRTAEEVDAALAKPGTTMVVVNS
                     ICGCAAGKMRPGVRLALQNSVVPDQSITVFAGQDREATERARSYFEGNPPTSPAIAIL
                     RDGKLVYLMQRFIIENNTAQGIAQELARAFNEFCAKTTV"
     gene            704..1720
                     /locus_tag="VFA02_07630"
     CDS             704..1720
                     /locus_tag="VFA02_07630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZU09759.1"
                     /translation="MASASVLTKQAPLTFQELLFRLQQFWAERGCVLQQPYDVEVGAG
                     TMSPDTFLRVLGPKPISIAYAQPSRRPADGRYGENPNRLYKHTQFQVILKPPPADIQE
                     VYLESLAAIGIDLREHDIKFEEDNWESPTLGAWGIGWQVMLDGLEITQFTYFQQCGGI
                     DLDPISGEITYGLERIAAFLQDVDSIYDIVWARDPVTGRAITYGEVRLEEELQFSVYN
                     FEAAEIDKLWEHLRMYEAECQALLSSFQDLKKLESRQKAHDRLVHEQTPASEEELEKT
                     MRRFPILGAYDLCLKCSHLFNLLDARGAISVTERVGVIARIRNLAVGIAKAYVAQQER
                     ALSC"
     gene            1807..3909
                     /gene="glyS"
                     /locus_tag="VFA02_07635"
     CDS             1807..3909
                     /gene="glyS"
                     /locus_tag="VFA02_07635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZU09760.1"
                     /translation="MSDFLFEIGLEEVPARMIAAAQDELSRRVLELLERERLLDVSHV
                     SKSYSTPRRLAVFVSGVLPKQADTEEQLTGPSWNVAFKDGVPAPAAQAFAKKAGVEVS
                     ALKKIATPKGEYAAATVLRKGRAASEVIAEQLPKELASLYWPKNMYWRKGKPERFVRP
                     VKWLLAILDHAIVPVGFAGVHAGNLSYGHRILHGGHAVTINQPYEYVSALESAKVLVD
                     VETRRHRIRKVLDAATRAISGARWREDTALVDTVTHLTEWPSVLLGNFESEYLALPEE
                     VLVTVMRDHQKYFAVEDASGRLAPHFLAVLNTETDEQGIAVIRHGNERVLRARFNDAR
                     FFWTVDQKVPLADRVEMLRSVTFQKDLGSYYDKTQANLSIAEQIAHLAEERGTALDKS
                     ALLTAVHLAKTDLTTELVKEFTELQGVVGGLYARAQGLGETVAQAIYSQYAPASVEDA
                     IPATAEGQILGLADRIQTIAAMFAIGLEPTGSKDPFALRRAANGVVKILAESELPLTI
                     SDLIAVAGSSGISITDSLGIFLRERVEFYLRDVRGSSYDVVNAVLAAGYDNVRDAIAR
                     AEALSAVRGSDDFVAISAAFKRIKNILRQAAEKQSIQGGAIDQKLLTDAAEQSLYREA
                     EKLAPSVEKFRVDRKYQSALEQIATLRPHVDLFFDKVMVMAEDPAVRENRLALLAWVL
                     NGFSSVADFSEIVVAQTG"
BASE COUNT         1040 a         1124 c         1321 g          998 t
ORIGIN      
        1 acaaaccagc gagcaggccc gcctatgccg aaggttgtat aaggggcaag cgggacatct
       61 tcaagcacac gcatcagctc caagatatca gtagaataag aacagtgacc ggacagcatg
      121 tccggagtgg aggagcagaa acgtgtatcc cgaaataatg gtggtcccaa tgcgcgaaga
      181 gctgacccgc gttggtatta aggaaacgcg caccgcggaa gaagtagatg cggcactggc
      241 aaaacctgga acaacgatgg tagtggtgaa ctccatctgc ggatgcgcag ccggcaagat
      301 gcgccccgga gtacgcctcg cgctgcagaa ttcagtggtc ccggaccaat ccattacagt
      361 atttgccgga caagaccggg aggccacgga acgcgcccgc tcgtatttcg agggcaaccc
      421 tccaacttca cccgcaattg cgattctgcg tgatggcaag ctcgtctatt tgatgcagcg
      481 cttcatcatc gaaaacaata cagcacaagg gatcgcccag gaactcgctc gcgccttcaa
      541 tgagttctgc gcgaaaacta ctgtttagtt tgcctgtgcg aatggttcag gactccccgg
      601 gactactcag tcgtgagcgt tccggggcct gccgtctcct gaatttttcc atcctttaaa
      661 atcaactcag caccaatttc aattgcgata gagagacttg cgcgtggcct cagcctccgt
      721 tctgacgaaa caagcaccgc ttacctttca ggaattgctt ttccggctcc agcagttctg
      781 ggccgagcgc ggatgtgtgc tccagcaacc gtatgacgtg gaagttggcg caggcacgat
      841 gtctccggac acgtttctgc gcgtgctggg gccaaagccc atcagcattg cctatgcgca
      901 gccatcgcgg cgtcctgcgg acgggcgtta cggcgaaaac ccaaaccggc tctacaaaca
      961 cacgcaattt caggtcattc tgaagccgcc accggcagac attcaggagg tgtatcttga
     1021 gtcactcgcg gcgattggta ttgacctgcg tgaacacgac atcaagttcg aggaagacaa
     1081 ctgggagtcg cctacactcg gcgcgtgggg cattggttgg caggtgatgc ttgatggcct
     1141 ggaaattacg cagttcactt acttccagca atgcggcggc attgaccttg atcccatctc
     1201 gggcgagatt acctatggtc tggagcgcat tgcagcattc ctccaggacg tggactcgat
     1261 ctatgacatt gtgtgggcgc gcgatccggt gaccggacgg gcgataacgt atggcgaggt
     1321 gcggctggaa gaggaactgc aattttctgt ctacaacttt gaggcagcgg aaatagacaa
     1381 actatgggag catctgcgca tgtatgaggc ggagtgccag gcgctgcttt caagttttca
     1441 ggacttgaaa aagctggagt cacgccagaa agcgcatgac cgtctggtcc acgagcagac
     1501 accggcatct gaagaagaac ttgagaagac catgaggcgc tttccgatac tgggtgccta
     1561 cgacctgtgc ctgaaatgct cgcacctgtt caatctgctg gacgcccgcg gagcgatttc
     1621 cgtaactgag cgcgtgggcg tgattgcgcg tatccgcaac ctggcggtcg gcatagcaaa
     1681 agcgtatgtg gcgcagcagg agagagcttt gagctgctag cgtttacctg ctggcttgtg
     1741 cggggactgt acagatattg aatagaaaga gtcaggagct aaaagcgaat agctgtcagc
     1801 taagatatgt cagattttct gtttgaaatt gggcttgaag aggttcccgc gcggatgatt
     1861 gcggcggcgc aggatgaatt gtcgcggcgc gtactcgagt tgcttgagcg ggagcggctg
     1921 ctggatgtat cgcatgtgtc aaagagctat tccactccgc gccgccttgc tgtttttgtc
     1981 tcgggagtgc tcccgaagca ggctgatacg gaagaacaac tgacggggcc ttcttggaac
     2041 gtagcgttca aagatggtgt gcctgctccg gcggcgcagg catttgcgaa gaaagccggc
     2101 gtcgaagttt ccgcgctcaa gaagattgct actccgaagg gcgaatatgc agcagcaacg
     2161 gtgttgcgca aggggcgcgc agcttctgaa gtcatcgctg agcaactccc aaaggaactg
     2221 gcttcgcttt attggccgaa gaatatgtac tggcgcaagg gcaagccaga gcgctttgtg
     2281 cgtccagtga agtggctttt ggcaattctg gaccatgcaa ttgtgcctgt tgggtttgcc
     2341 ggagtccatg ccgggaacct gagctatggc catcgcattc tgcacggcgg gcacgctgtt
     2401 accatcaacc agccgtatga gtatgtgtct gcgctcgaat cagccaaggt gctggtggac
     2461 gtagaaaccc gccgccatcg tattcgcaag gtcctggatg cggccactcg cgccatttcc
     2521 ggcgctcgct ggcgcgaaga tacagccttg gtggacaccg taacgcatct gaccgaatgg
     2581 ccatctgtgc ttctgggcaa ctttgagtct gaatacctgg cgcttccgga agaggttctg
     2641 gtgacagtga tgcgcgacca ccagaaatat tttgcagtgg aggatgcgtc cggcaggctg
     2701 gcgccacact ttcttgccgt gttgaatact gaaaccgatg agcagggaat tgccgtcatc
     2761 cggcacggaa atgaacgtgt attgcgcgcc cgcttcaatg atgcgcgctt tttctggact
     2821 gtggaccaga aggtcccact tgcggaccgt gtggagatgc tcaggtcggt gacgttccag
     2881 aaagacctgg gtagttacta cgacaaaaca caggcaaacc tgtccattgc cgagcagatt
     2941 gctcatctgg cagaagagcg tggcactgcg ctggacaaaa gtgcattact gaccgcagtg
     3001 catctggcga agaccgactt aacgaccgag ctggtcaaag agtttaccga actgcaaggc
     3061 gttgtcggcg gcctttatgc gcgggcacag ggactgggcg aaaccgtcgc gcaggccatc
     3121 tactcacaat acgcacctgc ttccgtggaa gatgcaattc cagctacggc agaagggcag
     3181 attctcggtc ttgcggaccg catacagacc attgcagcca tgttcgcgat tggtttggaa
     3241 ccgactggat cgaaggaccc atttgcattg cgccgtgcgg caaatggggt ggtgaagatt
     3301 ctggcggaat cggagttgcc gcttaccatc tctgacctta tcgcggttgc aggatcgagt
     3361 ggtatctcga ttacggattc tttaggcata tttctgcgtg agcgcgttga gttctatttg
     3421 cgcgatgtgc gcggatccag ctatgacgtg gtgaacgctg tgctcgcagc gggatatgac
     3481 aacgttcgtg atgcgattgc ccgcgccgag gcgctgagtg ctgttcgtgg atcggatgac
     3541 tttgtagcca tatcggctgc atttaaacgc atcaagaaca tcctgcgcca ggcggcagag
     3601 aagcagagca ttcagggagg agcgattgac cagaagctgt tgacggatgc ggcagagcaa
     3661 tcgctttatc gtgaagctga aaagctggct ccctccgtgg aaaagtttcg ggtagaccgc
     3721 aaatatcagt cggcgttgga gcagattgct acgctgcggc cgcatgttga cttgtttttc
     3781 gacaaggtga tggtcatggc ggaagatccg gctgtgcggg agaaccgtct tgctcttctg
     3841 gcatgggtgc tgaatgggtt ttcttccgtt gccgattttt ctgagattgt ggtcgcacag
     3901 actggttgaa tatcggccaa ttgactgcga tatcaagcat agaaaaaaat tctattgaca
     3961 gtcgtattca gaatcagata aacagtgttc ctgacaatgt taacggtttc attccggatt
     4021 ggaaggggcc cggaagcgat actgaagaac aatgtagtgt gaagtaaatg tattcgttta
     4081 ccttttgatg tacacgaata cattcaatca ggaggcgtta gcgagtagaa ccaatacaac
     4141 atagggacgc tttaccagaa ggcacaggcg catgaaaatg tgcgcgtctg aaaataaagc
     4201 taacgaatgc gcagagtggt acgcagttcc agacagacac tttccaggga ggttggtgta
     4261 tgcaaagtaa tgcccacggg cctactgcca ggtggcagag gctgttctat aaatatttac
     4321 cagcaatcgt tttcctgctg ctggttccat gtcttgcctt cgggcagatg gaccaaggca
     4381 gtgtcacagg catcgtccag gacacgaccg gagcggtcgt ccccaatgct caggtcaccc
     4441 tcaaaagtac ggataccggc ctggagcttc agacccacac gga
//
LOCUS       DASRBV010000199         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_676173, whole genome shotgun sequence.
ACCESSION   DASRBV010000199 DASRBV010000000
VERSION     DASRBV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676173"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFA02_07640"
     CDS             <1..318
                     /locus_tag="VFA02_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZU09761.1"
                     /translation="ELLTVPGAARKTASVVLGVAYGLAEGIVVDTHVLRISRRLHLTR
                     ETDPKKVEQDLMKIIPRDRWISFSHEVIHHGRQTCIARKPKCAECPLETLCTSADKTW
                     SSY"
     gene            462..1226
                     /locus_tag="VFA02_07645"
     CDS             462..1226
                     /locus_tag="VFA02_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09762.1"
                     /translation="MVRKQFLLITALVSTGLLFTGCNKSSSNNQEQPPQQQPAAAPSS
                     QPSATQPAQASPSSRERERTREREQPAATSERAAAPAAQPIVVPAGTALSVRLGTALS
                     SKTSTAGESFTGTLANSVVVNGVTAIPAGSTVTGTVTDAKSAGRFKGAAALALTLQSV
                     TVRGNSYPVDTTTFSQQTTGKGKRTATMVGGGAGLGALIGGLAGGGKGAAIGALAGAG
                     AGTAGAGLTGNNRDITLGPETVLSFRLQNSLTLPAR"
     gene            1404..2570
                     /locus_tag="VFA02_07650"
     CDS             1404..2570
                     /locus_tag="VFA02_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZU09763.1"
                     /translation="MTGPTQQALSSAPDLLFSICTPEQRASESGEKQARLTALMNKLD
                     LDAVLLRRNENIAWATSGQVETLVVIPGETGVASLLLARDGRKYYLTTNNEAPRLADE
                     EFSGLNYEPVIAPWYEDNTLAIARKALGSSRIGTDLPLDSFPIIDLAPLRSPLTSAEI
                     SRFRWLARNTAEATAQTLLDLEPGMTEEEMSALVAQRLLSRGILPSVLLMAVDDRIRK
                     YKHAVTRGGVLERFGMLNLCARKWGLAVSITRFVHFGKPPQELVDGFAACAEINARLL
                     HASRNGATSAELYAVAERAYREAGFPGEEKLHHQGGPAGYLEREWVATPKGTQKIADP
                     QALAWNPSIRGAKAEDTALLANDTIEILTQTPTLPRIDASVDGTRYPAAGVLVR"
     gene            3163..>4888
                     /locus_tag="VFA02_07655"
     CDS             3163..>4888
                     /locus_tag="VFA02_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZU09764.1"
                     /translation="MRKPLHCAISCVLAILVSSAAFAQGPVPDSPAIEQKVDSMLKKL
                     TLEQKIDLIGGVDGMYIRSEDAAGFPRLKMSDGPYGVRTWGPDTAYAAGIGLAATWDP
                     QLAQRMGVAIGEDARARGVHFLLGPGVNIYRAPMGGRNFEYFGEDPFLAGHIAVGYIE
                     GVQSQGVIATVKHFAANNEEYDRHNVSTDVDERTLREIYLPAFEAAVKVAHAGAVMNS
                     YNLLNGVHATQNCHLNNDILKKGWGFDGILMSDWMATYDGVGAANCGLDLEMPSGAFM
                     NRKDLLPAIKDGKVSEAVIDDKVRRIFRTAIRFGFLGRDQTDLSIPYDNPEGRAVALD
                     EARESITLLKNDGNLLPLEAGKIKTLAVIGPDAWPAVPGAGGSSQVEPYSAVSIMSGL
                     VNFPGSGIKVLYARGIPTTKELFQRTHFGTEQTMTSSSDPFWEDFFGKPTVKVETFDN
                     PNFSGTPTVTDAKHIDSWGAHEFTPPGEGKHSIRYTADYLPRRTGEYIVVAAGNWGDS
                     WKLLLDGKPVMEQPHREGQAPQSTRLHLTAGKAVHVQFDYVPDGNHVLAGVGIIAVDD
                     LVSPEAKKI"
BASE COUNT         1019 a         1318 c         1502 g         1049 t
ORIGIN      
        1 gagctgctca ccgtgcccgg tgcggcgcgc aagacggcca gcgttgttct tggcgtcgct
       61 tatggtcttg cagagggtat tgttgtagat acgcatgttc tgcgcatctc acgccgcctg
      121 catctcacac gggagacgga ccccaaaaaa gttgagcagg atttaatgaa gatcattccc
      181 agggaccgtt ggatctcctt ctcgcatgaa gtcatccatc atggacggca gacatgcatt
      241 gcgcgcaaac caaagtgtgc agaatgccct cttgaaaccc tgtgtacatc cgcagacaag
      301 acatggagca gctactgagg catcctgatt ttgatgtcct gcaatcagag agtcatcgcc
      361 ccgagatttc tctgcatcgg tgtgcagaga tgaccaaaca gtggtaatct tcgggcgatg
      421 tgctgcaaaa acccactgta cgaagaaagg gtgaaatctc tatggtccgc aaacagtttc
      481 ttctgataac cgcgctggtt tccactggat tgctgtttac aggatgcaac aaatcttcct
      541 caaacaacca ggaacagccg ccacaacaac agccagccgc agctccgtcc agtcaaccat
      601 ccgcaaccca accagcgcag gcaagcccat cttcccgtga gcgcgaacgc acgcgagagc
      661 gggaacagcc tgctgcgacc tctgaaaggg ccgcggcccc tgctgcacag ccgatcgtgg
      721 tgcccgccgg tactgcgctg tctgtccgtc tggggactgc tctcagctcc aagaccagca
      781 ctgccggaga aagctttacg ggaacgctgg caaactccgt tgttgtgaat ggggtgaccg
      841 ccattccggc aggctctacc gtgaccggaa ctgtaactga cgcgaaatcg gcaggaaggt
      901 tcaagggcgc ggcagcactg gccttgacat tacagtcggt gaccgtgcgt gggaacagct
      961 atccggttga cactacgacc ttcagccagc agaccacagg caaaggcaag cgtaccgcta
     1021 cgatggttgg cgggggcgcc gggcttggtg cgctcattgg cggacttgcc ggaggcggca
     1081 aaggcgcggc cattggcgct ctggctggcg cgggcgctgg cacagcaggt gccggactga
     1141 ccggaaacaa ccgcgacatt accctggggc ctgaaaccgt cctcagtttc cgtcttcaga
     1201 actcgctgac gctcccggca cgctgaaggc attgcttctc caccttgccc catgggaaga
     1261 cgcattcaga tgcgcccggt tttgaggtgc ggctggatgc tcttcccgta tggctcctat
     1321 tttccccttt gcgttccagg ctggcaccat gtacagttgt cccttagaaa ccctttaggg
     1381 aaatcgtttt acttatcagg gacatgactg gaccaacaca gcaggcactc tcctccgctc
     1441 ccgacctcct gttttctatc tgcacaccgg aacagcgcgc cagcgaaagc ggagaaaaac
     1501 aggcgcggct gactgcactg atgaacaaac tggatttaga tgccgtcctg ctccgccgca
     1561 atgaaaacat tgcctgggcg acatccggtc aggtagagac gctggtggtc attccaggcg
     1621 aaacgggtgt ggcttccctg ctgctggcgc gggacggccg caagtattac ctgacgacca
     1681 ataatgaagc gcccaggctg gccgatgaag agttttccgg cctgaattac gaaccagtca
     1741 tcgctccctg gtatgaggac aatacgcttg cgatcgcgcg taaggcgctg ggttccagca
     1801 ggatcggtac agatctgcca ctggacagct ttcccatcat agaccttgcg ccgctgcgct
     1861 ccccgctgac cagcgcggaa atttcacgct tccgctggct ggcgcggaac acggccgaag
     1921 ctacggcgca gacgctcctg gatctggaac ccgggatgac ggaagaagag atgtctgcgc
     1981 tggtcgcgca acggcttttg tcgaggggta ttctgccatc tgttctgctg atggccgtag
     2041 atgaccgcat ccgcaaatat aaacatgcgg tcacgcgtgg cggtgtgctg gagcgttttg
     2101 gaatgctgaa tctttgcgcg cgcaagtggg gactggccgt gtccatcacg cgctttgtgc
     2161 atttcgggaa gcctccacag gaactggtgg acggatttgc cgcgtgcgct gaaatcaacg
     2221 cgcggcttct gcatgccagc cgtaacggag cgacgtctgc agaactctat gcggtagcgg
     2281 agcgtgctta ccgggaagcg gggttcccgg gcgaagaaaa gctgcatcac cagggtggtc
     2341 ctgcgggtta tttagagcgc gaatgggtgg ccactcccaa gggaacgcag aagattgccg
     2401 atccgcaggc gctggcgtgg aaccccagca ttcgcggagc caaagccgag gacactgcgc
     2461 tccttgcaaa tgacacgatt gaaattctga cccagacccc cacgctgcct cgcattgatg
     2521 ccagtgtgga tggaacccgc tatccggcgg cgggtgtttt ggtccggtag gcttctgggg
     2581 accgtctctt tttttgtaaa ttcttaaaaa gaaaagagtt gcatgggtgg gtcctgttca
     2641 gcatggaagc tgggatgccc tacatctttt caagatgcgg gcatccggca catcgctgag
     2701 attcttcctg cctgtggtcg tcagaatggc gggctttgcc aatacgaaaa aatgccatca
     2761 ggtgctgctg ctactccaca tcttgaaaag gtgtggggga tgtgcggttt gttttttcgt
     2821 aaattcgtca aaacaggaga gttgctttcg gctgcggcgt ggatggtggg gacggttcaa
     2881 aagcggtcag cttaaagccg gcggctaaaa tgcgcaaggc ccgctttgtg ggcgcgggcc
     2941 ttttgggagg atttttaccc tctattttca gtatagctgg ctgggagggg tgttccggca
     3001 tttccggctg ttggctatct cttcctgcgg gaaggagtta gagggttttg ttactttcgt
     3061 gatgcttgac aggcgtgtgc cagactgcaa atccatctgc tttccaattc agcttttctt
     3121 ttgttaggct tgggcttctg ctttttcctg gaggcaaccg ctttgcgtaa accattgcac
     3181 tgtgctattt cgtgtgttct ggccattctg gtctcttctg ctgcttttgc acaaggccct
     3241 gtgccggaca gtccggcgat tgaacagaag gttgattcga tgctcaaaaa gctgacgctg
     3301 gagcagaaga ttgacctgat tggcggtgtg gatgggatgt acatccgctc agaagacgcg
     3361 gcggggtttc cacggttgaa gatgtccgac gggccttatg gcgtgcgcac ctggggtccg
     3421 gacacggcgt acgctgccgg aatcggactg gccgcgacat gggacccgca gcttgcccag
     3481 cggatgggtg ttgccattgg cgaggatgca cgggcgcgcg gcgtccactt tctgctgggg
     3541 ccgggcgtaa acatctatcg agcgccaatg ggcgggcgca attttgagta tttcggcgaa
     3601 gacccgtttc tggcagggca cattgctgtg ggttatatcg aaggcgtgca gagccagggc
     3661 gtgattgcca cggtgaagca tttcgcggcg aacaatgagg aatacgaccg gcacaacgta
     3721 agcaccgacg tagacgagcg cacactgcgg gagatttacc tgccggcatt tgaggcggcg
     3781 gtgaaagtgg cgcatgcggg cgctgtgatg aactcgtaca atctgctgaa cggcgtccat
     3841 gccacgcaga actgccatct gaacaatgac attctgaaaa aaggctgggg ctttgacggg
     3901 atcctcatgt ctgactggat ggcgacgtac gacggcgtgg gcgcggcaaa ctgcggactg
     3961 gacctggaga tgccctcggg cgcatttatg aaccgcaagg acctgcttcc tgcgatcaaa
     4021 gacggcaagg tttccgaggc ggtgattgac gacaaggtac ggcgcatctt tcgcacggcc
     4081 attcgctttg gatttctggg ccgcgaccag actgacctga gcattcccta tgacaatcct
     4141 gaaggacgcg ccgtagcgct ggatgaggca cgcgagagca tcacgctgct gaagaacgat
     4201 ggcaatcttc tgccgcttga ggcaggaaag ataaagacgc tggctgtgat tgggccggat
     4261 gcctggcccg cggtgcccgg tgctggaggc agttcgcagg tggagccgta ttcggcagtg
     4321 agtattatga gcgggttggt gaactttccg ggttccggaa tcaaggtcct ctatgcgcgt
     4381 ggcattccaa cgacgaagga gctttttcag cgcacgcact tcggcacaga gcaaacgatg
     4441 acctcttcgt cggatccctt ttgggaggac tttttcggta agccgacggt aaaggtggag
     4501 accttcgata atccgaactt cagcggcact cccacggtca cagatgcgaa gcatattgat
     4561 tcctgggggg cgcatgagtt tacacctccg ggggagggca agcacagcat ccggtacacg
     4621 gcggactacc tgccacgcag gacgggcgag tacattgttg tggcggcggg aaactggggc
     4681 gactcgtgga agctgcttct ggacggcaag ccggtgatgg agcaaccgca ccgcgaaggc
     4741 caggcgccgc agtccaccag gctgcatctg acggcgggca aggcggtcca tgtccagttt
     4801 gattatgtgc cggatggaaa ccatgtgctg gctggagtcg gcatcatcgc agtagatgac
     4861 ctggtctcac cggaggcaaa gaagatcg
//
LOCUS       DASRBV010000200        11625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_65126,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000200 DASRBV010000000
VERSION     DASRBV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65126"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFA02_07660"
     CDS             complement(<1..278)
                     /locus_tag="VFA02_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09765.1"
                     /translation="MRAFILSLSVLLPVALAQGARPCYSPDEAIAHANKDICISAHVY
                     DVVEMADGTRFLDVCKPDLPDDECRFTIVSRKADRKNVGDLELYRNQD"
     gene            complement(334..1191)
                     /locus_tag="VFA02_07665"
     CDS             complement(334..1191)
                     /locus_tag="VFA02_07665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU09766.1"
                     /translation="MQTDQTIAPDSTAVRVALWRALHLQVDAPPHVLNDNIGFQLAAP
                     GEDWRDRPDMNPLFTKLFRAAIVARARFIEDMVTAQANRGVSQYVILGAGLDTFAQRR
                     PEIASRLRIFEIDRPGPQAWKRRRLIELGLGVPDWLRFVPVDFESGDSWWDRLAETGF
                     DSSQPAVVVSTGVSMYLTREANAATLRQVSMLAPGSVFAMTYLLPLEFADPEERIGYE
                     AAIKGARASGTPFLSFFTAQEIEALARESGFKDARHVSAADLTQRYFSGRTDGLHPGK
                     SEEILLATT"
     gene            complement(1207..1545)
                     /locus_tag="VFA02_07670"
     CDS             complement(1207..1545)
                     /locus_tag="VFA02_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZU09767.1"
                     /translation="MTLNDFRRLALSLPEAREAEHMGHPDFRVAGKIFATLAYPDASF
                     GMVELSPLDQERFVKAAPAVFVPVPGYWGRNGATRVHLKKATKTVLKPAMEAAWKRAA
                     SRKSTAKLLD"
     gene            complement(1604..2062)
                     /locus_tag="VFA02_07675"
     CDS             complement(1604..2062)
                     /locus_tag="VFA02_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU09768.1"
                     /translation="MSPVKPIPDGYHSVQPYLHVRGAAEAIEFYKKAFGAKERMRMPA
                     PDGRLQHAEIEIGNSCIMLADESPDKQFYSPKHLGGSSVSIMLYVKDCDAIYKQALQA
                     GAISLREPADQSYGDRMSGVEDPFGFQWWIATHIRDVSLEEMQAATHSHA"
     gene            2369..3055
                     /locus_tag="VFA02_07680"
     CDS             2369..3055
                     /locus_tag="VFA02_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09769.1"
                     /translation="MATAASLPDAPGAQQTQTPVSKSQQDEAAEELKREEKQRILGVV
                     PNFNTVNSGDAAALTPKQKLHLFWKSSTDPFVFVAAAADAGISQAEDDFPGYGQGAEG
                     YFKRFGASYADTFDGNLWGNAILPIVLHEDPRYFRKGTGPVMGRILYAAKTNVWSRRD
                     NGTWGPNYANIAGNFIAGGISNLYYPSSDRGPGLTVQRALTVTAEGALGSLLVEFWPD
                     VSHKLFKKKN"
     gene            3152..6271
                     /locus_tag="VFA02_07685"
     CDS             3152..6271
                     /locus_tag="VFA02_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09770.1"
                     /translation="MSAISRRFFLAIIFLWSASAAFAASRTVVFYQAGFPSADSPAVS
                     ETALHAAFADAEFAGASQLASALEDPETNLLVMPYGSAYPEAAWPAILRYLDRGGNLL
                     ALGGKPFTRAAYSSGTGWLLRAPSVAASLELFIHDYQQTPGSDGLAFEPNHDVQPEIK
                     AFKWKRAFSPVIRLSVINKYSDGGTTGDNDSEITTLAWGTKDGHKLAAPVFEIGHNRY
                     RFVGGRWIFVACEPDANFFSNAQELQALAQLATRQNDRFTLRPRLPLFLAGEPLELQY
                     DGPSVGDQLQIKVTAEEGGKPVEITVPADAAHSIVLPAEAASGTGLHVIEATLLREGR
                     PLRAYRSGFWMRDWNYLLTGPKLGVGPDYFTLDGKPLPVVGTTYMSSDVQRMYLIQPN
                     AYVWDRDMAQIHGDGLNMIRTGLWTAWEPLLNSNGEVSEAALRTIEAFLMCARHHHLP
                     VQFNLFAFYPGNFGGQNGYLDPAGLHAQEVFVRSLAARFHAVPFLAWDLINEPSANGN
                     LWRELPQGDVFEQEAWRRWLAKHYSDQATLLDDWAEPSLGIGRDLQLQPTTASPEVAA
                     QDPLALPKAGAWNFDPVRSGYNPLKVYDYSLFTQDIFADWVKRTKDIIRESGSQQLVT
                     VGQEENGVANRLSPAFYSPLIDFTADHTWWDFDAILWASLAAKFPGKPMLIQETGEQR
                     RLTQRDHLRFSPEIEGWQLERKIAAAFAQGAGALEWVWNVNAYMANDNEIPIGAVRPD
                     GTEKPEARVLEDYAAFIAKSPQSFTRILPPDVTIVTSQALQYSGQDELAIAVQENSLR
                     ALCYYDHTPARMLPENRLAELGQPKLVILPAAQALTDAAWQQLLDYVSKGGTLLVSGP
                     VSKNEHWQTVDRPGELGVKAELLPLSVRQSALVLPGQQPILVTYSGHVQQSPVSVLHF
                     ADGKSVEAVSHRNGRILWAADPVEMAEGYAQSAALYEYALHEAGVKPAFSGTLSPGVM
                     AFPTVLDDAVLYSFSNESLEDQTVDIRDAVSGARLRFTLGAQRGGLVLVSRKDGKMIA
                     ASDAIEGMK"
     gene            complement(6346..7095)
                     /gene="tpiA"
                     /locus_tag="VFA02_07690"
     CDS             complement(6346..7095)
                     /gene="tpiA"
                     /locus_tag="VFA02_07690"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZU09771.1"
                     /translation="MRKALIAANWKMYKTPAQAAEYVKAFLPLVTGHDRDEIVLCPSD
                     TSLSTVIPAVAGSNVAVGGQNMHWAEEGAYTGETSAVMLKAIGATHVIIGHSERRQYF
                     NETDETVNKKLVTALKHKLVPIVCIGEVLAEREAGQTGDVLRRQTSLALAGIKPEEAQ
                     PIVIAYEPVWAIGTGKTATPEIAAEAHLTIRSEIARLLGREVAGNIRILYGGSVKPDN
                     ATALLSQPEIDGALVGGASLDPQSFAKIVKY"
     gene            complement(7253..8467)
                     /locus_tag="VFA02_07695"
     CDS             complement(7253..8467)
                     /locus_tag="VFA02_07695"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZU09772.1"
                     /translation="MSKLSIRDLDLQHKHVFMRVDFNVPLTEDGSEITDDTRIRETLP
                     TIEYALRHKAKLILASHLGRPKGKPNPKYSLRPVVDRLRVLLDHQLGESVNVAFSPDC
                     VGDVAKELARQLESGQVLLLENLRFHAEEEANDPNFAKQLASLCGLYVNDAFGSAHRA
                     HASTEGITHHVSKSAAGLLMEKELQYLGKALTEPAKPFVAILGGAKVSDKIKVIDNLL
                     GKVDALLIGGGMAYTFLKAKGQDVGKSLVEADKLDIAKAALKKAEEKGVRFLLPVDHV
                     LADKFAPDAKTQIFAGDGPFPAEWMALDIGPKTVELFANEIAEARTIVWNGPMGVFEM
                     PAFAHGTTKIAKAVAKNSEAISIIGGGDSVAAVKQAGVADQITHISTGGGASLEFLEG
                     RKLPGVEALTEK"
     gene            complement(8474..8857)
                     /locus_tag="VFA02_07700"
     CDS             complement(8474..8857)
                     /locus_tag="VFA02_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU09773.1"
                     /translation="MRQNISGSSPYEPVIGFSRAVRVGNAVHVSGTAPVGADNADPAA
                     QTRQCLSLIQSALEKAGAKLEDVVRTRMYLTHSEDWEAVGRVHGEFFGKIRPAATMVV
                     VAKLLNPSWRVEIEADAVISNSESQ"
     gene            complement(9017..10021)
                     /gene="gap"
                     /locus_tag="VFA02_07705"
     CDS             complement(9017..10021)
                     /gene="gap"
                     /locus_tag="VFA02_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZU09774.1"
                     /translation="MAVKVGINGFGRIGRNVFRASLGNPDIDIVAVNDLTSPKTLAHL
                     LKYDSILGNLNNEITAGEDYISVDGKKIKVFAEKDPAKLPWDSVGAQVVVESTGRFTD
                     ASAAKAHLQGSVKKVIISAPATNEDITIVLGVNDDKYDPAKHNVISNASCTTNCLAPV
                     VKVLHENFGIVSGIMTTVHSYTNDQVVLDFPHKDLRRARAAALSIIPSSTGAAKALKL
                     VIPEMAGKLDGFAMRVPTPNVSVVDLTFIAEKPATVQSVNESLKKAAETNLKGILGYE
                     ENELVSSDFKGDPRSSIVDAPLTKVVGNSVKVISWYDNEWGYSNRVRDLILYVAKKGL
                     "
     gene            10305..11123
                     /locus_tag="VFA02_07710"
     CDS             10305..11123
                     /locus_tag="VFA02_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09775.1"
                     /translation="MRRRSKRGPNTSESTGKIGQELPANQPPPLQPNYADEMPQSAEK
                     VEETAAVVPDEPTGAFADAAEPQPGPQPEPIGKYEIETQPESPSTPPPEPQATPQRSP
                     KGYVVLAIGLPGSGKTTWFKRRGVTPLSSDLLRTILFDDITEQRYQGLVFSTLRSLLR
                     ARLIAKMPWNYVDATNLSPHERRQWIKMAKSFGYDVQAVFFDVPFEVCMERNRRRERV
                     VSDEVMHKMAERLRPPNFKEGFSKITVVRVKGAPASQPAAEASGESALEGESAS"
     gene            11257..>11625
                     /locus_tag="VFA02_07715"
     CDS             11257..>11625
                     /locus_tag="VFA02_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09776.1"
                     /translation="MEERFPFIGTMSAAIEFQNVTLTTSDGRRLLDGIRLKMEEGTTT
                     AILGRSGSGKTTLLRMVNRMVLPASGCIVVQGNDIAHQDAVLLRRGIGYVIQETGLFP
                     HFTVERNAGLVPELNGNHADK"
BASE COUNT         2413 a         3059 c         3528 g         2625 t
ORIGIN      
        1 atgtcctggt tgcggtaaag ctcgagatcg cccacgtttt tccggtcggc cttccggctg
       61 acgatggtga agcggcactc atcgtccggc agatcaggtt tgcagacatc gagaaagcgg
      121 gtgccatcgg ccatctcaac cacatcgtag acgtgcgcgc ttatgcaaat gtctttattg
      181 gcgtgcgcga ttgcttcgtc cggcgaataa cacggacgcg cgccctgcgc gagtgcgact
      241 ggtaacagca cagaaagaga gagaataaag gcacgcatgg aaacgtgcct caaagataag
      301 cgaacctgga gcagcagaac aggttacgaa agattatgtt gttgcaagca aaatttcttc
      361 agattttcct ggatgaagac cgtctgtcct gccggagaaa taacgctggg taagatcggc
      421 tgctgaaaca tgccgggcat ctttgaaacc agattcgcgg gccaacgctt caatttcttg
      481 tgccgtaaag aagctgagga agggtgttcc gctggcacgc gctcctttga tggctgcttc
      541 gtatccaatg cgctcttcag gatcggcaaa ttccagcggc agaagatagg tcatggcaaa
      601 tacagagcct ggagcgagca tggagacctg gcgcagggtg gccgcatttg cctctctggt
      661 gagatacata ctgacgccgg tggaaacaac caccgctggc tggcttgaat caaagcccgt
      721 ctctgcgaga cggtcccacc aggaatcgcc cgactcgaaa tccactggga caaaccgcag
      781 ccagtccgga acgccaaggc cgagttcgat aaggcgccgg cgcttccatg cctgcgggcc
      841 tggacggtct atctcaaaaa ttcgcaggcg ggaagcgatc tctggcctgc gctgagcgaa
      901 ggtgtccagc ccggcgccga gaatgacata ctggctgacg ccgcggttgg cctgtgcggt
      961 gaccatgtct tcgataaacc tggcgcgggc cacaatggcg gcgcggaaga gttttgtaaa
     1021 tagcggattc atgtccgggc ggtcgcgcca atcttcgccg ggggccgcaa gctggaagcc
     1081 aatattgtca ttcaggacat gtggtggcgc atccacctgg agatgcaagg cccgccacag
     1141 ggcgacgcga actgctgtac tatccggagc aatggtttgg tcagtctgca taggtccttt
     1201 gattgatcag tcaagaagtt ttgctgttga ttttctggag gcggctctct tccatgcggc
     1261 ttccatcgct ggttttaaaa cggtcttcgt tgctttcttc agatgtaccc gtgttgctcc
     1321 atttctaccc caatagccgg gaacaggaac aaagacagca ggagcagctt tcacgaacct
     1381 ctcctggtca agtggagaga gctcgaccat tccaaagctc gcgtcaggat aagcgagcgt
     1441 tgcaaagatt tttcctgcaa cgcggaaatc aggatgaccc atgtgttcag cttccctggc
     1501 ttcaggaaga ctgagtgcga ggcggcgaaa atcattgagc gtcatggatt ttgtactcta
     1561 tcctgccgga cggctgcttc cggcaggatg gcaggaagca catttaagca tgagagtgag
     1621 tagcggcctg catttcttcc agggacacgt ccctgatgtg cgtagcaatc caccactgga
     1681 acccgaaggg atcttcgaca ccggacatgc ggtcaccata ggactggtcg gctggttcac
     1741 gcaggcttat tgctccggct tgaagggcct gtttgtagat ggcgtcgcag tccttcacat
     1801 agagcatgat gctcacggat gatcctccga gatgctttgg gctgtagaat tgcttatccg
     1861 gggattcatc ggccagcatg atgcaggagt tgccaatctc aatttcagcg tgctgcaagc
     1921 ggccatcagg agcaggcatg cgcatgcgct cttttgcgcc gaacgctttc ttgtagaact
     1981 caatggcttc ggccgcgcct cgaacatgca ggtacggctg aacactgtgg tatccgtctg
     2041 gaatgggttt cactggggac atagcgtttt ctcctttacg ctgcttattt tcacgcaggg
     2101 gcaaggaagc gcgattgtaa aaaattgacc tcctgcaccg tgttcagcgc caggtaacct
     2161 attcaagcca gaacagtgca ggattttaag agactttcgt gttccaatat acgtccttag
     2221 gtgaggtccc ccgcgacata ttgttgcaga ttggaaatga atgcattctt tttttctaag
     2281 acgttggttt gttccagcgg tcatttttct ggttcttgag gcctgccccg tggaggcgca
     2341 cgcgcagggt acttcccaaa ctgaaacagt ggcaacagcc gcctctttgc ctgatgcccc
     2401 cggtgcccag caaacccaga cccctgtttc caaatcgcag caggacgagg cagcagagga
     2461 gcttaagcgg gaggaaaagc agcgcattct tggtgtggtc ccgaacttta atacggtgaa
     2521 tagtggcgac gctgctgcac tgacgccgaa acagaagctt catcttttct ggaagagttc
     2581 gactgaccca tttgtctttg tcgcagcggc tgctgatgcg gggatcagcc aggcggagga
     2641 cgatttcccg ggatatggcc aaggggcaga aggttacttc aagcggttcg gcgcctcgta
     2701 tgctgatacg tttgacggca atctctgggg caatgcgatt ctgccgatcg tgctgcatga
     2761 ggatccgcgt tatttccgca aaggaacagg ccccgtcatg ggccggattc tttatgcggc
     2821 gaaaacaaat gtctggtctc gccgcgacaa tgggacgtgg gggcctaact atgcgaatat
     2881 tgcaggcaat ttcatcgcgg gcggcatctc gaatctgtat tacccatcct cagaccgcgg
     2941 cccgggcctg actgttcagc gtgcgctcac ggtaacggct gaaggagcat taggctcatt
     3001 gctggtggag ttctggccgg atgtctcgca caagctgttc aaaaagaaaa attgagacag
     3061 acgggaaatc agacgccgcg gcacagaagc attcaggcga tgcgttcagg cacgttatga
     3121 taccgttatc aacggaacct gaattatcgt tatgagcgcc atctcccgcc gtttttttct
     3181 tgcaattatt ttcttatgga gtgcttcggc agcctttgct gcttcacgca cggttgtgtt
     3241 ttatcaggcc ggatttccat ccgcagattc acctgctgta agcgaaactg cgttgcacgc
     3301 ggcctttgcg gatgctgagt ttgccggggc gtcgcaactg gcctcagcgc ttgaggaccc
     3361 tgaaacgaat cttcttgtga tgccgtatgg ctcggcgtat ccggaagcag cgtggcccgc
     3421 gattctgcgc tatcttgacc gtggtggcaa cctgctggcg ctgggaggga agccgtttac
     3481 gcgggcggcg tattcatccg gtacagggtg gctgttgcgt gcgccgagtg ttgcggcgtc
     3541 gctggagttg tttattcatg attatcagca gacgccgggt tcggatggtc tggcgtttga
     3601 gccaaaccac gatgtgcagc cggaaatcaa agcattcaaa tggaagcgcg cctttagccc
     3661 tgtgattcgg ctttctgtca tcaacaagta cagtgatggc ggaacgaccg gagacaacga
     3721 ctcggaaatc accacgcttg cctgggggac aaaagatggg cacaaactgg ctgctccggt
     3781 ctttgagata ggccataacc gctaccggtt tgtgggtgga cggtggatct tcgttgcctg
     3841 cgagccggat gcgaacttct tctcaaacgc gcaggaattg caggcgcttg cgcaactggc
     3901 gacgcggcag aacgatcgct ttacgctgcg tccgcggctg ccgctgtttc tggccggaga
     3961 accgttggaa ttgcaatatg acgggccttc agttggcgat caactccaga tcaaagtgac
     4021 ggcagaagag ggtggcaagc ctgtcgaaat aacggtgcct gccgatgctg cgcattccat
     4081 cgtgctgcct gcggaggcag cttcagggac gggtctgcat gtgattgaag ccactctgct
     4141 gcgcgaaggc aggcctttgc gagcttatcg cagcggcttc tggatgcgcg actggaatta
     4201 tctgctcact ggcccgaagc tcggcgttgg cccggattat ttcacgctgg atggcaagcc
     4261 gctgcctgtg gtcggcacga cgtacatgtc gagcgatgtg cagcgcatgt atctgattca
     4321 gccgaacgct tacgtctggg accgtgacat ggcgcagatc catggcgatg ggctgaacat
     4381 gatccgtaca gggctgtgga cggcttggga gccgctgctg aattccaatg gcgaagtcag
     4441 tgaagcggcg ctgcggacga tcgaagcttt tctgatgtgt gcgcgtcatc atcatctgcc
     4501 ggtgcagttc aacttgtttg ccttctatcc cggcaatttt ggcggacaga acgggtatct
     4561 tgatcctgcc gggctacacg cgcaggaggt ctttgtgcgg tcgctggctg cacgcttcca
     4621 tgctgttccg tttctggcat gggacctgat caacgaaccc agcgcaaacg gcaatctgtg
     4681 gcgcgagctg ccgcaggggg atgtttttga acaggaggca tggcgcagat ggctggcgaa
     4741 gcactattcc gaccaggcaa cattgctgga tgactgggcc gagccttcgc tgggtattgg
     4801 gcgcgatttg caattgcagc ctacgacagc atcgccggaa gtagcagcac aagaccccct
     4861 ggcgctgccg aaggccggag cgtggaactt cgatccagtg cgctcggggt acaatccgct
     4921 gaaagtctat gactattctc tgttcacgca ggacatcttc gcagactggg tgaagcgcac
     4981 caaagacatc atccgggaga gtggctcgca gcagcttgtc accgttggac aggaagagaa
     5041 cggcgtagcc aaccggcttt ctccggcctt ctattcaccg ctgattgact ttactgccga
     5101 ccacacctgg tgggactttg acgcgattct ctgggcctcc ctggccgcga aatttcccgg
     5161 taaaccgatg ctgattcagg agacaggcga gcagcgccgc ttgacacagc gcgaccatct
     5221 gcgcttcagc ccggaaatcg aaggctggca actggagcgc aaaatcgcag cggcctttgc
     5281 gcaaggcgcc ggcgcgctcg aatgggtgtg gaacgtgaac gcctacatgg cgaacgacaa
     5341 cgagattccc attggagctg tgcgtcctga cggaacagag aagcccgaag cgcgcgtgct
     5401 cgaagactat gccgcgttca tcgccaaaag cccgcagagc ttcacacgca ttttgccacc
     5461 ggatgtgacc atagttacat cgcaggcgct gcaatacagc gggcaggatg agctggcgat
     5521 tgctgtgcag gaaaactcac tgcgcgcgct ctgctattac gaccacacgc ctgcgcggat
     5581 gctgccggaa aatcgtcttg ctgaactggg ccagccgaag ctggtgattc tgcctgcggc
     5641 gcaggcactc acggacgcgg cgtggcagca gttgctggat tatgtcagca aaggcgggac
     5701 gctactggtt tccggccctg tctcaaagaa cgagcattgg caaacggtgg accgccctgg
     5761 tgaattgggg gtgaaggcgg agttgctgcc gctctctgta cgtcagtctg cattggtcct
     5821 gcccgggcaa cagcccattc ttgttaccta ttcaggacac gtccagcagt ctccggtaag
     5881 cgtgctgcat tttgcagatg ggaaatctgt tgaagccgtg agtcacagaa acggccgcat
     5941 cttgtgggcg gcagacccgg ttgagatggc cgagggctac gcgcagagtg ccgcgctata
     6001 cgaatatgcg ctgcacgaag caggcgtgaa gcccgcattt agcggaacac tctccccggg
     6061 agtgatggcc tttcccaccg tgctggacga tgctgtcctc tacagcttct ccaatgaatc
     6121 gctggaagac cagactgttg acatccggga tgcggtgagt ggggcgcggt tgaggtttac
     6181 attaggagcg cagcgcggcg ggctggtcct ggtgagcaga aaagatggaa agatgatagc
     6241 ggcctctgac gcgattgagg gtatgaagta atacagcacg catggcgtgc gagtgcccca
     6301 cactcttgca agggacatgg ggcacccggc ctggacagcc agtccttagt acttcacaat
     6361 ttttgcgaaa gattgcggat cgagactggc tccgcctacg agagcgccgt caatttccgg
     6421 ctggctgagc agtgcggtgg cgttgtccgg ctttacgctt ccgccgtaga gaatgcggat
     6481 gttgccggcg acctcgcgcc caagaaggcg ggcgatttcg ctgcggatgg tgaggtgcgc
     6541 ttcggctgcg atctctggcg tggcggtttt tcccgttccg atggcccaga ctggctcata
     6601 agcaatcaca atgggctgcg cttcttccgg cttgattccg gcgagggcca gcgaggtctg
     6661 gcggcgcaga acatcgccgg tttgtccggc ttcgcgctcg gcaaggactt ctccaataca
     6721 gacgataggg acgagcttgt gcttgagggc tgtgacgagc ttcttgttca cggtctcatc
     6781 ggtttcgttg aagtactggc ggcgctcgga gtgcccgatg atgacgtggg tcgcaccgat
     6841 ggccttgagc atcactgccg acgtctcacc cgtgtaggcg ccttcttctg cccagtgcat
     6901 gttctgtccg ccgacagcta cgtttgagcc tgcgacggca gggatgaccg tgctcaggga
     6961 cgtgtctgaa gggcagagga cgatctcgtc ccgatcatgt ccggtaacga gaggcagaaa
     7021 ggccttgacg tactcggcgg cctgggccgg ggtcttgtac attttccagt tggctgcaat
     7081 gagtgctttg cgcatgtttg tttaaaccct ttgctgaatc atttttttta cgtctcgtca
     7141 ttctgaagac cctgagcaat gcgaagattc ttcacttcgc tgcgctacgt tcagaatgac
     7201 ggcatccgat ttcgtacccg acaagtcgcg ctgaatctga tgaagccaat aactatttct
     7261 ccgtcagcgc ctcgacacca ggcagtttcc tgccttccag aaactcgagt gaagcgccgc
     7321 cgccggtaga gatgtgcgtg atctggtcgg cgactccagc ctgttttacg gctgcaaccg
     7381 aatcgcctcc gccgatgatg gaaatggctt cgctgttttt tgctacggct ttggcgatct
     7441 tcgtggtgcc gtgtgcgaag gcaggcatct cgaagacgcc catcgggccg ttccagacga
     7501 tggtgcgggc ctcggcgatt tcatttgcga acaactcgac tgttttcggg ccgatgtcga
     7561 gcgccatcca ctctgcgggg aaggggccgt cgccagcgaa gatctgggtt ttggcgtccg
     7621 gcgcgaattt gtcggcgagg acgtggtcta caggcagcag gaagcgcacg cctttttctt
     7681 cagctttctt cagcgcggct ttggcgatgt ccagcttgtc ggcttcgacg agggacttgc
     7741 cgacgtcctg gcctttggct ttgaggaagg tataggccat gccgccgcca atcagcagcg
     7801 catctacttt gcccagcaga ttatcaatga ccttgatttt gtcggatacc tttgcgccgc
     7861 ccaagattgc gacgaagggc ttggccggtt cggtcaatgc cttgcccaga tactgaagct
     7921 ctttttccat cagcaagcct gcggcagact tgctgacgtg atgcgtaatg ccttccgttg
     7981 aagcgtgggc gcgatgggcg ctgccgaaag cgtcattcac ataaagcccg cagagcgagg
     8041 cgagctgttt ggcgaaattc ggatcgttgg cttcttcttc agcgtgaaag cgcaggtttt
     8101 cgagcagcag aacttgtcct gactcaagct ggcgggccag ttcctttgct acatcgccga
     8161 cgcagtccgg ggagaaggcg acgttcacgc tttcgccaag ctgatggtcg agcagcacgc
     8221 gcaggcggtc cactacaggc cgcaggctgt actttgggtt cggtttgccc ttggggcgtc
     8281 cgagatgcga ggccagaatc agctttgcct tgtggcggag cgcgtactca atggtgggca
     8341 gggtctcgcg gatgcgcgtg tcatcggtga tttcgctgcc gtcttcggtg agcggcacat
     8401 tgaagtccac acgcatgaaa acgtgtttgt gctgaagatc aagatcgcgg atagatagtt
     8461 tggacataga gattcactgg ctttctgaat tggaaattac ggcgtcggct tcgatttcga
     8521 cgcgccatga tggattgagc agctttgcga caacgaccat ggtcgccgca gggcggattt
     8581 ttccaaagaa ctcgccatgc acgcggccta cggcctccca gtcttcggag tgggtcagat
     8641 acatgcgcgt gcggacaaca tcttcgagtt tcgctccggc tttttcaaga gcagattgaa
     8701 tcagggaaag gcactggcga gtctgtgcgg cagggtcagc gttgtctgcg ccgactggcg
     8761 cggtgccgga aacatgcaca gcattgccaa cgcgcacggc gcgggagaag ccgatgaccg
     8821 gctcataggg tgaagaacct gagatgttct ggcgcatcgg tggctgctcc tggagagaga
     8881 aggaatttat tatcgcagat ggatgccgtc attctgagga gtggaacggc gaagaacctt
     8941 tgcgatgatg gctaaactaa aaccgctaag attcttcgct tcgcccagaa tgacgcatct
     9001 ctgccgtttg tactgattac aggccctttt tagcgacgta tagaatgagg tcgcggacgc
     9061 ggttggaata gccccattca ttgtcgtacc aactgatgac cttgaccgag ttgccgacga
     9121 cttttgtcag cggcgcatct acgattgagg agcgcggatc gcccttgaag tcgctggaca
     9181 ccagctcgtt ttcctcgtag ccgagaatac ccttcaggtt ggtctcagcg gccttcttca
     9241 gagattcatt tactgactgc acggtggcag gcttttctgc gatgaaggtc aggtccacga
     9301 cggagacgtt cggggttggg acgcgcatgg caaagccatc gagcttgcct gccatctcag
     9361 ggatcaccag cttgagggcc ttggcggcgc cagtggagct gggaatgatg ctcagagcgg
     9421 cagcgcgggc acggcgcagg tccttgtgcg ggaagtcgag cacgacctga tcattggtat
     9481 agctgtggac cgtggtcatg atgcctgaga cgatgccgaa gttctcatgc aggaccttga
     9541 cgacaggcgc gaggcagttc gttgtacagg aggcgttgga gatgacattg tgctttgctg
     9601 gatcgtactt gtcatcattc acgcccagca caatggtgat gtcctcattg gttgccggcg
     9661 cggagatgat gacttttttc actgaaccct gcaggtgggc cttggctgcg ctggcgtcgg
     9721 taaagcggcc ggtggattca acgacgacct gtgcgccgac cgagtcccag ggcagcttag
     9781 cagggtcttt ttcggcaaag accttgatct tcttgccatc tacagagata tagtcttccc
     9841 cggcagtgat ttcgttgttg agattgccca ggatcgaatc gtatttaaga agatgggcca
     9901 gggtcttggg gctggtgagg tcgttgactg cgacaatgtc tatgtccgga ttgccgagcg
     9961 aggcgcgaaa tacgttacgt ccgatacggc caaaaccgtt gatgccaacc ttaactgcca
    10021 ttcaaaaagt tctccttaag gtttttgttg aagggtctga gttttgagag gctgatccag
    10081 agagtgatga tgggccgccg ccggagcgcc actcaataat gatgctagca ctgtcgtcaa
    10141 tatcaagaaa gcctctggcg cacatcagca aaatttattt cgccggggag cggggatgcg
    10201 actggagtcc aggccgtgaa atttcctgtg attttggcgg atgtttgctc tgaaggcggt
    10261 gcctatgtta catccacatc atgcgcctat ggatgcgcga tcgaatgaga agacgctcga
    10321 agcgtggacc gaatacttcc gagtccacag ggaaaattgg gcaggaactg cctgctaacc
    10381 agccgccacc gctgcagcct aattatgcgg atgaaatgcc gcagagtgca gaaaaggtgg
    10441 aagaaactgc tgctgttgtt ccggatgagc ctaccggcgc ttttgccgat gccgcagaac
    10501 cgcagccggg accgcagcct gaaccaatcg gaaagtacga aatagaaaca cagcctgagt
    10561 cgccctcgac tcctccgccg gagccgcagg caacgccgca gcgcagtccc aagggatatg
    10621 tggtgctggc catcggactg cctgggtcag gcaaaacaac atggtttaaa cgccgtggcg
    10681 tcactccgct ttcgagtgac ctgctgcgga cgatcctttt tgatgacatt acggaacagc
    10741 gctaccaggg gctggtcttt tctaccctgc ggtcactgct gcgcgcgcgg ctgattgcca
    10801 aaatgccgtg gaactatgtg gatgccacca atctgtctcc gcatgagcgg cggcagtgga
    10861 tcaagatggc caagagcttt ggctatgatg tgcaggccgt attttttgat gtgccatttg
    10921 aggtatgcat ggagcgcaac cgccgccgtg agcgcgtggt gagcgatgaa gtgatgcaca
    10981 agatggccga gcgcctgcgt ccgccgaact tcaaggaagg cttctcgaag atcacagtgg
    11041 tgcgtgtgaa gggcgcacct gcttcgcaac ccgcagccga ggcttccggc gagagtgcgc
    11101 tggaaggcga aagtgcttcg taagagaagg aaatagactc gtaagccggg cagagatgtc
    11161 cggctttatt atttttccta tgccgtgggt gcccccaccc tgaagggatg tggattttgc
    11221 aggaaccatt ccccaggcgc ccattgcggt gaaagggtgg aagagcgctt tccatttatc
    11281 ggtactatgt ccgcagccat cgagtttcaa aacgtcaccc tcaccacgtc ggacgggcga
    11341 aggctgctcg atggaatccg tctgaaaatg gaagaaggca caacgacggc gatcctgggc
    11401 cgcagtggat cgggcaaaac tacgttgttg cgtatggtga accgcatggt gctccctgcc
    11461 agcggctgca ttgtggtgca gggaaatgat atagcccatc aggacgcagt attgctccgg
    11521 cgcgggattg ggtatgtgat tcaggagacg ggcctctttc ctcatttcac ggtcgagcga
    11581 aatgccggac ttgtgccaga gctgaacgga aaccacgccg acaag
//
LOCUS       DASRBV010000201         7131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_265358, whole genome shotgun sequence.
ACCESSION   DASRBV010000201 DASRBV010000000
VERSION     DASRBV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265358"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..576
                     /locus_tag="VFA02_07720"
     CDS             76..576
                     /locus_tag="VFA02_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZU09777.1"
                     /translation="MAGDNSFDIVSKVDLQEVRNAVEQAVKEIRTRFDLKDSHSEIHI
                     EGNDAIQLASADEYKLEAINEILRQKLVKRGISLKALTYGKVEPAAGSTVRQKISLQQ
                     GIPTEKAKDIVRIIKDSKKKAQASIQGDTVRVSSKDRDTLQEIIALLKSKDLGIDMQF
                     TNYRSN"
     gene            588..1577
                     /locus_tag="VFA02_07725"
     CDS             588..1577
                     /locus_tag="VFA02_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZU09778.1"
                     /translation="MSTTTIATAKEVFDLLHDDLRAIEREFGDDAISSVKAITEISEY
                     LREGGGKRIRPSLLLLSAKSQGYSGRGMVRLGAVVEMVHTATLVHDDIIDSADIRRGR
                     PSANTTWGNAKCVLAGDWLYMQAFRVALEERNFRVLDLLIGLTQQMVEGELLQMETLG
                     RPVTEQEYNELIYRKTACLFEVSMRLGSVLAGVNGEAEARMGEYGHALGMAFQIVDDV
                     LDLTATEEVLGKPVASDLREGKATLAVIHSLENGPGADREAILTVLTDRNFNRVTHDE
                     ILAILARNGSVDYAMNTAFQYAEKARAALATLPDSDYRRALLWVPDFVVAREK"
     gene            complement(1596..1970)
                     /locus_tag="VFA02_07730"
     CDS             complement(1596..1970)
                     /locus_tag="VFA02_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU09779.1"
                     /translation="MQDNKQAKPKVLVADDERVIADTLAIILNQSGFEATAVYSGEKA
                     VEVAEAIKPDMVISDVIMTDLNGIDAAIKIRAMLPSCKILLFSGQAATADLLDRARNQ
                     GHEFEILAKPVHPQDLLAKLKA"
     gene            2241..3587
                     /locus_tag="VFA02_07735"
     CDS             2241..3587
                     /locus_tag="VFA02_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZU09780.1"
                     /translation="MAEAIIRAQQVEKFYAQPSENRIQVIAPTDLAIVPGEIVALLGP
                     SGSGKSTLLRMLSGLSRPSAGQVFWHEKPIGSVEINVSIVFQSFALFPWLTVLENVEA
                     PLKARGIDPETRKERSLKILDTVGLDGFQSAYPKELSGGMRQRVGFARALVVEPEVLF
                     MDEPFSALDVLTAENLRSELLELWQKKTIPTQAIFIVTHNIEEAVLLADRIIVLGRNP
                     GHVRTDFKVALHHPRDKKNPAFTQLVDYIYTVLTRPDVTPDVPRLHVDGRRIRDQREM
                     RYEMLPHARPGGIAGLLEIIIDFGGRADVYRLADELSFELDDLLPIVEAAQLLGFLKL
                     EEGDVSITAEGREFAESEILKQKELFREAALNHVLLLRQITRALSNKSDHTVPEEFFL
                     DMLDEQFSEEETQRQLETAINWGRYAELFDFDATRRRFVLPEVEETAATMEGSEGE"
     gene            3584..5323
                     /locus_tag="VFA02_07740"
     CDS             3584..5323
                     /locus_tag="VFA02_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZU09781.1"
                     /translation="MKVRYTLSRSLVVERSWPFFLDLTVAFGLLAGFYAVLQIARYWM
                     GGAVPEVEISQSPRMLPIYAFYSVVRIGVAYLLSLLFAVVYGYIAAYNRRLEALMIAT
                     LDILQSIPVLSFLPGVMLAMMSLIPSRQLGIEFGAILLIFTGQVWNMAFSFYSSLKSI
                     PRELQEACTINRFSRWQRLWQLELPYAAIGLVWNSMVSVAGGWFFLMVCEMFPVGNRQ
                     FRLPGLGSYLQTAASTGNSLAMTYGLLTMVAIIVLTDQLVWRPVIDWSDKFKFENVEA
                     SDRVKSPVLAVLRHSNMLAAIRQHTIIPLSENIYRNLASLSANKHTSTGSDQRRKGPS
                     VLVWLLLVIVLCLVCFAAFKAILLLKQVPRAEVGTTLLGAIATFFRVNVSLFLAAAWT
                     IPVGVAIGFNPRLARIAQPLAQIAASFPATALFPVILIGLMKLGAGLGIGSIALMMLG
                     TQWYILFNVIAGAMAIPSEMREVATLFRFPDWQRWTTVILPGIFPYLITGLVTASGGA
                     WNASIIAEYFHLKDQTLHTLGLGAQISAASDAGNFPILLLATIIMALMVVTINRLIWR
                     PLYHLAETRYRLE"
     gene            5560..>7131
                     /locus_tag="VFA02_07745"
     CDS             5560..>7131
                     /locus_tag="VFA02_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZU09782.1"
                     /translation="MSTATNTIPPKADPKRQETTIHGHPLVDDYAWLRDKEDPKTIAW
                     LNAENAWTAAVMEPAAELQEKLYREMLSHIRETDVSVPFREGDFWYYSRTEEGLQYPI
                     YCRKHETLDAPEIVILNVNKLAEGESFMAIGAFTVSDDGNLLAYSTDTKGFRQYTLHV
                     KDLRTGELLPERVERVGSIVWAADNKTLFYTVEDEVQKRQYQLYRHVLGTPHEQDVLI
                     YEETDERFNIGAGRTRDRKYIVLESGSHTTSEARFMPSDAPAGELRLIAPRRDNIEYY
                     LDHRNGLWFIRINDTGRNFRLVTAPVEDSAPQNWLELLPHRSEVMLEDIELFAGFYVA
                     CERENGLQHLYVRRFTGTSVETAPAQEIGFPEPVYSAHPHVNRTFETNAFRYAYQSLV
                     TPGSVYEYDINTAESKLLKQQEVPGGFDRTNYVSERIFATARDGVKVPISLVYRKDTF
                     EHGKNPLYVYAYGSYGYSLPVGFNSNRLCLLDRGFVMAYAHIRGGGDLGKPWHDAGRL
                     LQKRNTFTDFIAAVEH"
BASE COUNT         1566 a         2225 c         1792 g         1548 t
ORIGIN      
        1 aggtttttca atatccctcc ggacatggca cactgaaatt agcgcttttg tatccaccaa
       61 gagagagctg aggttatggc cggggacaat tctttcgaca tcgtaagtaa agtagacctt
      121 caggaagtgc gcaatgccgt cgaacaggcg gtcaaggaaa tccgcacacg ctttgacctg
      181 aaggattcgc actcggaaat tcacatcgaa ggcaacgacg ccatccagct cgcttctgcc
      241 gacgaataca agctcgaagc cataaacgaa attctgcgcc agaagctcgt caagcgcggt
      301 atctcgctga aggccctgac ctacggtaag gtcgagcctg ccgccgggtc cactgtgcgc
      361 cagaaaatca gcctgcaaca aggcattccc acagagaagg cgaaagacat cgtccgcatc
      421 attaaagatt caaagaagaa ggcccaggca tccattcagg gagacactgt gcgcgtcagc
      481 agcaaagacc gcgacaccct gcaggaaatc attgctctgc tgaagtcaaa ggacctcggc
      541 attgacatgc agtttacaaa ttaccggtcg aactagacgg agcgcgagtg agcacgacaa
      601 ccatagcgac ggcgaaagag gtcttcgatc tcctgcacga tgacctgcgc gccattgagc
      661 gcgagttcgg cgacgacgcc atctcctccg tcaaggccat tacggagatc tccgaatatc
      721 tgcgcgaagg tggaggcaag cgcattcgcc cgtcgctgct gctactttcc gccaaatcgc
      781 agggctattc cggacgcggc atggtccgcc taggagcagt cgtcgaaatg gtgcacaccg
      841 ccacgctcgt ccatgacgac atcattgact cagccgatat ccgccgcgga cgcccctctg
      901 ccaacactac ctggggtaac gccaagtgcg tcctcgccgg cgactggctc tacatgcagg
      961 cctttcgcgt ggcgctggaa gaacgtaact tcagggtcct tgacctcctc atcggcctca
     1021 ctcagcaaat ggtggaaggc gagctgctcc agatggagac cctgggccgt cctgtcactg
     1081 agcaggagta caacgagctt atctatcgga aaactgcctg cctctttgaa gtttcgatgc
     1141 gccttggctc cgtgctcgcc ggagtcaatg gcgaagctga ggcacgtatg ggcgagtacg
     1201 gccacgcgct cggcatggcc ttccagattg tggacgatgt gctcgacctt accgcgaccg
     1261 aggaagtgct cggaaaaccc gtcgccagcg acctgcgcga gggcaaagcc accctcgccg
     1321 tcatccactc gctcgaaaac ggccccggcg cggaccgcga ggccattctt accgttctga
     1381 ccgaccgtaa cttcaaccgt gttactcacg atgagattct ggccatcctg gcccgcaacg
     1441 gctcagtaga ttacgccatg aacacggcct tccagtacgc cgaaaaggca cgcgctgccc
     1501 tcgccaccct tcctgattcg gattacaggc gtgcactgct ctgggtaccg gattttgtag
     1561 tggcacgaga aaaataacgg cagtccgggc gcagtttaag ctttcagctt tgccagaagg
     1621 tcctgcgggt gtaccggctt agccaggatt tcaaactcat gcccctggtt gcgcgcgcga
     1681 tccagcaggt cggcggtggc cgcctgtccg gagaagagca gtatcttaca tgacggcagc
     1741 atggcgcgaa tttttatagc ggcgtcaata ccgttcaggt cggtcataat cacatcgctg
     1801 atgaccatgt ctggtttgat tgcttcagcc acttccacgg ccttttcacc gctgtaaaca
     1861 gcggttgctt caaatccgct ctggttcagg atgatggcca gcgtatcagc aatcacacgc
     1921 tcatcgtcag ccacaagaac cttaggcttt gcctgtttat tgtcttgcat cttctccgac
     1981 tctcttacct ggatcaaggg ttaggtacaa aaaggtctca atcgttaaga ccggttaaaa
     2041 aaatgaattc aaactcttcc ggccagataa atgatggctg tttcgcaaaa aactacacca
     2101 taagatgata cgctgtttgc aggaggatgc ttcatcttcc tgaaatccaa tccacttaca
     2161 gcagatccaa caaatcttca cgcgccgggt gggcgctttc ccgcccagag gtttacacca
     2221 tgtaaacggg gttccaggca atggctgaag caattattcg agcgcagcaa gtcgagaaat
     2281 tttacgctca accgagcgaa aaccgcattc aggtcattgc ccccacagac ctcgccatcg
     2341 taccaggtga gatcgtcgcc ctgctcggtc cctccggctc aggcaaatcc accctcctgc
     2401 gtatgctttc cggactatca agaccctctg ccggacaggt cttctggcat gaaaagccca
     2461 ttggctcggt tgagatcaac gtctctatcg tcttccagag ctttgcgctc tttccctggc
     2521 ttactgttct tgaaaatgtc gaggccccgc tgaaggcccg tggtattgat cctgagaccc
     2581 gcaaggagcg cagcctgaag atactcgata ctgttggcct tgacggcttc cagtctgcct
     2641 atcccaaaga actctccggc ggaatgcgcc agcgcgtcgg ctttgcccgc gccctcgtcg
     2701 tcgaaccgga agtccttttc atggacgagc cattttccgc cctcgacgtg ctcaccgcag
     2761 aaaacctgcg cagcgagctt cttgagctct ggcagaagaa gaccatcccc acgcaggcca
     2821 ttttcatcgt cacccacaat atcgaagaag ctgtcctgct tgccgatcgc atcatcgtgc
     2881 tcggacgcaa ccctggccac gtccgcaccg atttcaaagt cgccctgcac cacccgcgcg
     2941 acaaaaagaa ccccgcgttc acccagctag tggactacat ctataccgtg ctcacccggc
     3001 ccgacgtcac gcctgacgtc ccgcgcttgc acgtagacgg acgccgcatc cgcgaccagc
     3061 gtgagatgcg ttatgagatg ctgccccacg cgcgcccggg cggcattgcc ggactactcg
     3121 aaatcatcat tgacttcggt gggcgcgccg acgtctaccg tcttgcagac gagctttcct
     3181 tcgaattgga cgacctgctt cctatcgtag aagccgcgca actgctcggc ttcctgaaac
     3241 ttgaagaagg cgacgtttcc atcaccgccg aaggccgcga atttgccgaa tcagaaattc
     3301 tcaaacaaaa agagcttttc cgggaagccg cactcaatca tgttctgctg ttgcgccaga
     3361 ttacccgcgc cctcagcaac aaatccgacc acaccgttcc ggaagagttc ttcctcgaca
     3421 tgctcgacga gcagttcagc gaagaagaga cccagcgcca gctcgaaacc gccatcaact
     3481 ggggccgtta cgccgaatta ttcgactttg atgccacccg ccgccgcttt gttctgccgg
     3541 aagtggaaga gaccgccgca acaatggaag gaagtgaggg tgaatgaagg tccgttatac
     3601 cctttcgcgc tcgctggtgg tcgagcggtc atggccgttc tttcttgacc ttaccgttgc
     3661 ctttggtctg ctcgcaggct tctatgccgt tctgcagatc gcacgttact ggatgggtgg
     3721 cgctgttcct gaagtggaaa tctcgcagtc gccgcgcatg ctgcccatct atgccttcta
     3781 ctctgtggtc cgcatcggag tggcctatct gctgagcctg ctcttcgccg tggtctatgg
     3841 ctacatcgcc gcctacaacc gccggctgga agccctgatg attgccacac tggatattct
     3901 gcaatccatt cctgtgctca gcttcctgcc gggagtcatg ctggccatga tgtcgctcat
     3961 tcccagccgt cagttaggca tcgaattcgg cgccatcctg ctcatcttta ccggacaggt
     4021 ctggaacatg gccttcagct tttattcttc gctgaagagc attccccgcg aactgcagga
     4081 ggcctgcacc attaaccgtt tctcgcgctg gcagcgcctc tggcaacttg aattgcccta
     4141 tgccgccatc ggcctcgtct ggaactcgat ggtctctgtc gctggcggat ggttcttcct
     4201 catggtctgc gagatgtttc cggtcggcaa ccgtcagttc cgtcttcccg ggcttgggtc
     4261 ttaccttcag accgctgctt ccaccggaaa ttccctggcc atgacgtatg gcctgcttac
     4321 gatggtcgct atcattgtgc tgacagacca gttggtctgg cggcccgtca ttgactggtc
     4381 agacaaattc aaatttgaaa atgtcgaggc gtccgatcgc gtcaagtctc ctgtgctcgc
     4441 agtgttgcgg cactcgaaca tgctcgccgc cattcgccaa cataccatca ttcccctcag
     4501 cgaaaacatc tatcgcaacc tggccagtct gtccgcgaac aagcacactt ccactggatc
     4561 cgatcaacgc agaaaaggcc catctgtcct cgtttggctg ctgctggtga tcgttttgtg
     4621 cctggtatgt tttgccgcct ttaaagcgat tctgcttctg aagcaggttc cccgcgcgga
     4681 agtcgggacc accctgctcg gcgccattgc tacgttcttt cgcgtgaatg tctctctgtt
     4741 tctggccgct gcctggacca tccccgtcgg cgttgcgatt ggttttaacc ccagactggc
     4801 gcgaatcgcg cagccgctgg cgcagattgc tgcttctttc cccgcgaccg cgctctttcc
     4861 tgtcatcctg attggcctta tgaagctggg agcaggactc ggcatcggtt ccatcgccct
     4921 catgatgctg ggcacacaat ggtacatcct cttcaacgtg atagccgggg ccatggccat
     4981 cccctccgaa atgcgcgaag tcgccacact cttccgcttc ccggactggc agcgctggac
     5041 gaccgtcatt ctccccggaa tcttccctta tctcattacc ggactcgtca ctgcctccgg
     5101 cggcgcatgg aacgcctcca tcatcgccga gtatttccac ctgaaagacc agaccctgca
     5161 taccctcggc cttggtgccc agatcagcgc cgccagcgac gccggaaatt tccccatcct
     5221 gttgcttgca accatcatca tggccctcat ggtggtcact attaaccgcc tcatctggcg
     5281 cccactctac cacctcgcag aaaccaggta ccgcctggag taatttttat ttgttcaaac
     5341 gaataatgtc ctgtgcccgc tgcgtggttt gatattctga atatagggag aaagctacgc
     5401 aagccggata cccctctgcc aaacctcagc agcgttttgc cgtcaacacc tctgtaaaaa
     5461 atcggaagcc acgcatcact ctgggaacgt gccagatgct tcataactcc aatgtttcta
     5521 agattttggg aaaaagtagg gggagggtat cctgaaaaga tgagcaccgc aacgaacacc
     5581 atccctccca aagcagaccc caaacgccag gaaacgacca tccacggcca tccccttgta
     5641 gacgattatg catggctccg cgacaaagag gatccgaaga ccatcgcctg gcttaatgcc
     5701 gaaaatgcct ggaccgcggc cgtgatggag cctgctgccg aactccagga aaagctctac
     5761 cgcgagatgc tcagccacat cagggaaacc gacgtctcgg tccccttccg cgaaggagac
     5821 ttctggtatt actcccgcac cgaagaggga ctgcaatacc ccatctactg ccgcaaacac
     5881 gagaccctcg acgcgcccga gatcgttatc ctcaacgtga acaaactcgc cgagggcgag
     5941 agttttatgg ccatcggggc cttcaccgtc tccgatgacg gaaacctcct tgcctactcc
     6001 actgacacca aaggcttccg ccagtacacg ctgcacgtga aagacctgcg taccggcgag
     6061 cttctgcccg aaagagtcga acgcgtaggc tccatcgtct gggcagcgga caataagacc
     6121 ctcttctaca ccgtggaaga tgaggtgcag aagcggcaat atcagctcta taggcatgtc
     6181 ctgggcactc cgcatgaaca ggatgtcctc atttacgaag aaacagacga gcgcttcaat
     6241 atcggagcag gacgcacgcg cgaccgcaaa tacatcgtcc ttgaatcagg cagccatacc
     6301 acgagcgagg cccgtttcat gccatccgat gcgccggcag gtgagttgcg ccttattgct
     6361 ccgcgccgcg ataacatcga gtactatctt gaccaccgca atggactctg gttcatccgg
     6421 ataaacgaca ctggacgcaa tttccggctc gtgactgctc cagtcgaaga ttcagctccg
     6481 caaaactggc tggaactgct tccgcataga tcggaagtta tgcttgaaga tatcgagcta
     6541 tttgcaggat tctacgtagc ttgcgagcgc gagaatggac tccagcatct gtacgttcgc
     6601 agattcaccg gcactagtgt tgaaaccgct ccagcgcagg aaatcggctt ccccgagccg
     6661 gtctacagcg cgcatccgca cgtcaaccgc accttcgaga caaacgcttt tcgctatgcc
     6721 tatcaatcgc tggtcacgcc aggctcggtc tacgaatacg acatcaacac agccgaatca
     6781 aaactgctca agcagcagga agtccccggc ggattcgacc ggacaaacta cgtctccgag
     6841 cgcatcttcg ccaccgcgcg cgatggcgta aaagtgccca tctcgctggt ctaccgcaaa
     6901 gatacattcg agcatggcaa aaatccgctt tacgtctacg catacggctc ctacggctat
     6961 tcccttcccg ttggcttcaa ctccaaccgc ctttgcctgc tcgatcgtgg ctttgtgatg
     7021 gcctacgctc acatccgcgg cggaggtgat ctcggcaaac cctggcacga tgccggacgc
     7081 ctcttgcaaa aacgcaacac cttcaccgac ttcatcgccg ccgtcgaaca c
//
LOCUS       DASRBV010000202         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_469754, whole genome shotgun sequence.
ACCESSION   DASRBV010000202 DASRBV010000000
VERSION     DASRBV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469754"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..884)
                     /locus_tag="VFA02_07750"
     CDS             complement(114..884)
                     /locus_tag="VFA02_07750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU09783.1"
                     /translation="MACLQGKVAIITGASSGIGQEIAKRFGKEGASVAIDYVSHPEGA
                     EATRKAIDQAGGKAITVQADITKKEDRERLVAETWKAFGFADILVNNAGTEKRSDFWE
                     MKEEDYDRVMNVNLKSAVFLTQIFVRRLRDAKRPGRIINISSVHEDMAFPGFTAYCCS
                     KGGMRMFARNLAVELGPMGITVNNIAPGAIATPINQSLLEDKQKLEALVANVPLHRLG
                     TPEDVAGMAAFLASDDAAYVSGSTFVIDGGLMRNYHEQ"
     gene            complement(903..1835)
                     /locus_tag="VFA02_07755"
     CDS             complement(903..1835)
                     /locus_tag="VFA02_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZU09784.1"
                     /translation="MESAGKVRIGISGWRYAGWRGRFYPAKLAQKRELEYASREFSSI
                     EINGTFYSMQRPETFARWAAETPENFVFAIKGSRYITHQRRLREIEEPLSNFFAQGLL
                     RLNEKLGPVLWQFPPNFTFDFSRFERFFRLLPLTTTAAAQMAEAHGPALRGRNWTEID
                     RQRPMRHCVEIRHESFVVPEFIDLLRKYKIGLVVADTVEWPLLMDVTSDFIYCRLHGS
                     EQLYASGYDDAALDIWANRVTAWAEGGNPSDGRFAHPEKAHEVKGRDVYVYFDNDAKV
                     RAPFDAKGLQERVEKRAGEEKVIIPAPAHGVCRI"
     gene            complement(1893..2726)
                     /locus_tag="VFA02_07760"
     CDS             complement(1893..2726)
                     /locus_tag="VFA02_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZU09785.1"
                     /translation="MAHPHAAAPTPTALPGVANIVAIGSGKGGVGKTTIAVNSAIALA
                     KLGYKVGLIDADVYGPNVPLMLGTGQQPRVLANNMIEPNTAHGIKVISIGFISPGDKP
                     MVMRGPMLHQIIRQFLQQVEWGELDFLLVDLPPGTGDVVISLVQTVPLTGAVVVSTPS
                     DVSLQDARKALEMFHQVNVEVLGIVENMSHFTCPHCHQEIDIFSKGGAERTAKQFNVP
                     FLGAVELDPEVRMGGDRGLPVALLGESSPKAKDFYAVAKQIAERAKAQSAKAENVFEI
                     S"
     gene            complement(2818..>3117)
                     /locus_tag="VFA02_07765"
     CDS             complement(2818..>3117)
                     /locus_tag="VFA02_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZU09786.1"
                     /translation="SSLNRAFNTSMEVGVKVWVENTIAGTHRHVASAYLTFVAVDSQG
                     RRVPVPKVQPETEEEKRRYEDAGRRREIRQKELELKRAARKGEVEVAGPVRPGRV"
BASE COUNT          701 a          952 c          708 g          756 t
ORIGIN      
        1 atcagcaaaa gaatcaaaat gatcagcaag atagttatca tagcaattgc cctccagact
       61 gctttaccgt ttgatggcag gtaaaggaaa ctggatatct acctctgttt tatttactgc
      121 tcgtgataat tccgcatcag gccgccatct atcacaaaag tagatccgct cacatacgcc
      181 gcatcatctg acgccagaaa tgcagccatc cctgctacat cttccggcgt ccccaggcga
      241 tgcaacggca catttgcaac aagggcttct aacttctgct tgtcttccaa taaggattga
      301 ttgataggag tagcaatggc tcccggagcg atgttattca cagtaattcc cattggccca
      361 agttctacag caaggttccg cgcaaacatt ctcattccgc ccttgctgca acagtaagca
      421 gtaaatccgg gaaaggccat gtcctcatgc acagagctga tgttgatgat gcgtccaggg
      481 cgctttgcat cgcgcaaacg ccgcacaaat atctgcgtaa gaaacactgc gctcttcagg
      541 ttcacattca tcacgcggtc atagtcttct tccttcatct cccagaagtc agaccgcttc
      601 tctgttccgg cattgttcac cagaatgtcg gcaaagccaa aggccttcca tgtttctgcc
      661 acaaggcgct cccggtcttc ttttttggta atgtctgcct gcacggtaat cgccttgcct
      721 ccggcctggt caatggcttt tcttgtggcc tcggcacctt ccggatgact gacataatca
      781 atcgcaaccg aagcgccttc tttgccaaag cgttttgcaa tttcctgccc tatgcctgag
      841 cttgcgcctg taataatcgc aaccttaccc tgtaaacaag ccatgatcgc tccctgcttg
      901 gatcagatgc ggcaaactcc atgcgcaggc gccggtatga tgaccttttc ctcacccgcc
      961 cgcttttcta cacgctcttg caaccctttc gcatcaaagg gcgcgcggac ctttgcatcg
     1021 ttgtcgaagt aaacatacac atctcgtccc tttacttcgt gcgctttctc cggatgcgcg
     1081 aaacgtccat cagaaggatt tcctccttct gcccaggccg tcacgcgatt tgcccatatg
     1141 tccagcgccg catcgtcgta cccactcgca taaagttgtt ctgaaccatg cagcctgcaa
     1201 taaataaaat cggaagtcac atccatcagc aacggccatt caactgtgtc tgcaaccaca
     1261 aggccgattt tgtatttgcg cagcagatct ataaattccg gtacaacaaa gctctcgtgg
     1321 cgtatttcaa cacagtgccg cataggacgc tgtctatcaa tctccgtcca gttccggccc
     1381 cgcaatgccg gcccatgcgc ttcggccatc tgcgctgccg ctgttgttgt caacggcagc
     1441 agacgaaaaa acctttcaaa tcgcgagaaa tcaaaagtga aatttggagg gaactgccac
     1501 agaactgggc caagcttctc gttcaggcgc aacagacctt gcgcaaaaaa gttcgacagc
     1561 ggctcttcta tctcacgcag acggcgctgg tgcgttatgt accgcgatcc ttttatggca
     1621 aaaacaaagt tttcaggcgt ttcggcagcc caccgggcaa aggtctccgg cctctgcatg
     1681 gagtaaaacg tgccgttaat ttcaatcgaa gaaaattccc ggctggcata ttccagttcg
     1741 cgcttctggg ccagttttgc cggataaaat cttccccgcc agcctgcata acgccacccg
     1801 gaaatcccga ttcggacctt gcccgcgctc tccattctgt tccatgtgga tgcgtggacg
     1861 gctggatgcc tttgcctttc agatgttgct tttcaggaaa tctcaaaaac gttctcggcc
     1921 ttcgccgact gtgcctttgc ccgttcagca atctgtttcg ccacagcata gaagtccttt
     1981 gctttcggcg aactctctcc cagcagcgca acaggcaatc cacggtcacc tcccatacgg
     2041 acctccggat caagttcgac tgcgcccaga aacggaacat tgaactgctt tgccgtgcgc
     2101 tctgctccgc ctttggagaa aatatcaatc tcctgatggc agtgcggaca cgtgaaatgg
     2161 ctcatgttct caacaatccc aagcacctcg acattcacct gatggaacat ctccagcgcc
     2221 ttgcgcgcat cctgcagtga aacgtccgat ggggtagaaa ccacaacagc tccagtcagc
     2281 ggcactgtct gcaccagcga gatgaccaca tcgcccgttc ccggaggcag gtctaccagc
     2341 agaaaatcga gttcacccca ctccacctgc tgcaaaaact gccgaatgat ctgatgcagc
     2401 atcggcccac gcatcaccat cggcttgtcc ccgggcgaga taaagccaat ggaaatcacc
     2461 ttgattccat gcgccgtatt cggctcgatc atgttgttcg caagcacacg cggttgctgg
     2521 cccgtaccca gcatcagcgg cacattgggc ccataaacgt cggcatcaat caggccgact
     2581 ttatatccga gctttgccag cgcaatcgcg gagttcactg caatcgtcgt cttccctaca
     2641 ccacctttcc ctgaaccaat ggcgacgatg ttcgccactc cgggaagcgc tgtaggcgta
     2701 ggtgctgccg catgtggatg tgccataaaa actcctgatt cattttgccg tcctggcctg
     2761 ggcgtagcgt aggggaaaac cctgcccttc ttcgaagaga caggacgatt gaaattgcta
     2821 tacgcgtccc ggacgcaccg gtcccgctac ttccacttct cccttgcgcg cggcgcgctt
     2881 cagctccagc tctttttgcc gaatttcccg gcgccggccg gcatcttcgt agcggcgttt
     2941 ttcttcttcc gtctcgggct gtactttggg tacaggaaca cgcctgccct gcgaatccac
     3001 cgctacgaat gttagatatg ctgaagccac gtggcggtgc gttccggcaa tcgtgttttc
     3061 tacccacacc ttcacgccaa cttccatcga agtgttaaat gcgcggttca gcgatga
//
LOCUS       DASRBV010000203         7194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_316249, whole genome shotgun sequence.
ACCESSION   DASRBV010000203 DASRBV010000000
VERSION     DASRBV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316249"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFA02_07770"
     CDS             complement(<1..315)
                     /locus_tag="VFA02_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09787.1"
                     /translation="MLSALPGLPARLRHCQGVLVDSNVLLDIATKDPRWGSWSADALA
                     ECAEYSILMINPIIYAEVSIGYSTIEALEAALPSGIYQRCPLPWEAGFLAGKCFLTYR
                     RRG"
     gene            complement(315..557)
                     /locus_tag="VFA02_07775"
     CDS             complement(315..557)
                     /locus_tag="VFA02_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZU09788.1"
                     /translation="MQITSKGQVTIPQEVRNRLGLLPHTEVEFEIAGDYALIRKARRA
                     ANPASRGNRAVELLRGTSDVRMSTDEIMSLTRGGKR"
     gene            1012..3129
                     /locus_tag="VFA02_07780"
     CDS             1012..3129
                     /locus_tag="VFA02_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZU09789.1"
                     /translation="MKSYQGSEIRNVAVVGHAHSGKTTLVSALLHAAKMTSTPEPVTA
                     YDEEDVARGTTMLNAVAYAEWSGVKVNFIDTPGFHMFAHEARCAMVPVESALVVVNAA
                     NGPEAVTDRVWSYADEFNLPRVAVINQMDHPRAEWQKSLETMRERYGRHLVPVQLPVV
                     DAEGFHGIVDLVTMEAFFYTPNGDGHGKIADIPANMAEEAQKAHEALVELVAEGKDEL
                     MEEFFAEGTIPEQHLITALHEAIREDRIFPVLFASGGTNVASDHLLDFLKVYAPAPVE
                     REPVAARAMLQAVAAHANGGGDESNPEIVMRRVDDGEPLALYVFKTMSDPFAGRISFF
                     KVFSGHVRNDAVVVNYSRKGTEKFSHLSIMQGRTLVPVGELHAGDLGAVAKLKDTFTG
                     DTLGEKGHEIFFEPVSLPEPAMTYAIEPKSRADEDKLAPAMHKLMEEDLLVRFYRDAQ
                     TNEFLIAGAGQPHIESLVSKLRKRYHTEVTLKSPKVPYRETIRGRAEAQGRHKKQTGG
                     HGQFGDCKIRMEPLPRGAGFEFVNDIFGGAIPRQFIPAVEKGIQEAAARGYLAGYPVV
                     DFKVTLYDGSYHEVDSNELSFKLAGRLAFRKCMEQAKPVLLEPIMRVEIEAPESCAGT
                     LMGDLNQRRGRVQGMESRGATTVIRAEVPMAEMLTYGQSLTSMTQGQGTFHMEMEHYD
                     IVPQPLAEKIIANAKRPQEEEEE"
     gene            3274..3786
                     /locus_tag="VFA02_07785"
     CDS             3274..3786
                     /locus_tag="VFA02_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU09790.1"
                     /translation="MTIAEVLLADFDSEIGNTRRTLERVPEDRPDYKPHEKSMPLGRL
                     AMHCATLPLFGYYIMEDDAMDLAAPKRPHADLTFRSRAECLAQLDETAAKCRAALASA
                     SDEHFQKKWPFTWGEHVISNETRLRTYRTMFFNHMIHHVAQLGVYLRLNHLAVPALYG
                     PSADEQWSPK"
     gene            complement(3798..5165)
                     /locus_tag="VFA02_07790"
     CDS             complement(3798..5165)
                     /locus_tag="VFA02_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HZU09791.1"
                     /translation="MMKRFLLVSLLLLSAALLLPAQARTPLLAPTPPMGWNSWDSYGL
                     TITEAQFKANVQWLNQHLKQYGWKYVVIDEGWYLQHPENAGKQGADQGYTLDSYGRYV
                     PAPDRFPSSVKGAGLKPIADYVHSLGLKFGIHIIRGIPKEAVEKNLPIAGSRFHAADA
                     ANTSDLCQWNPDNYGLKNNAAAQAYYNSIAKLYAGWGLDFLKVDCISSPYKADEIHMM
                     SAGLKNSGRPIVLSLSPGPTPISEAGDVRKYAQMWRISNDVWDLWNKPESDTNTFPQP
                     LRRQFKNLADWSSHIEPGHWPDADMLPLGYLGPNAGWGQPRHSRLSQDEARTLVTLWS
                     IARSPLVLGANLTQMDSFTESLLTNPEVIAVDQHSSANKPVIQTPDTVVWTAKGAAGK
                     QYVAAFNLGDTPQNISFSWKELEITAPGHVRDLWQRKDLGQMPGLKVTLPPHASVLYA
                     VGAVR"
     gene            complement(5220..6788)
                     /locus_tag="VFA02_07795"
     CDS             complement(5220..6788)
                     /locus_tag="VFA02_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZU09792.1"
                     /translation="MSAEQKSVYTREDLESIGFDPLRDLGFPGEFPFTRGIQPTMYRG
                     RLWTMRQYAGMGDAEESNRRYKFLLQQGTAGLSVAFDLPTQIGYDSDHPMSLGEVGKV
                     GVAIDSIEDMERLFDSIPLDRISTSMTINATASILLALYVAVARRTGANVKKLSGTVQ
                     NDILKEYIARGTYIYPIHHAMRIITDMFAWANEELPEWNTISISGYHMREAGSTAAQE
                     VAFTLANGMTYVQAAIDAGLDVDKFAPRLSFFFNAHNNFLEEVAKFRCARRVWAHIMR
                     SHFGAKHPRSWMLRFHTQTAGSTLTAQQPENNIVRTAIQALSAVLGGTQSLHTNGYDE
                     ALALPTEEAARIALRTQQIIACETGVAHTIDPFAGSYYIEALTQKLEQQVKTYLDRIA
                     ALGGMLRAIEQGWVQQEIQNSAYDYQRAVDAGQAVVVGVNRFTREDGPPIPIQRIDED
                     LERRQVERVRALRQRRDKARWQSSLERVKNHARSGQNLMPAILEAVESHATVGEIAAA
                     MREIFGEYQESVVI"
     gene            complement(6966..>7194)
                     /locus_tag="VFA02_07800"
     CDS             complement(6966..>7194)
                     /locus_tag="VFA02_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09793.1"
                     /translation="VAHASGIIWLQITGLFFSVFAIVFASNTWKLRQNWSAGPGHARF
                     LAFAAVTLVFAWFAGSSFYRAGRKQRSGHRR"
BASE COUNT         1449 a         1825 c         2398 g         1522 t
ORIGIN      
        1 gccacgcctg cgatacgtaa ggaagcattt gcctgccaga aagccggcct cccaggggag
       61 cggacaccgc tgatagatgc cggaaggcag ggccgcttcg agcgcctcga tggtggagta
      121 cccgatggac acttcggcat agatgatggg attgatcatc agtatggaat attcagcgca
      181 ctctgcgaga gcgtccgcgg accaggaacc ccagcggggg tctttggtgg caatatccag
      241 tagaacgttg ctgtctacca gcacaccctg gcaatgccgg agccgcgcgg gaaggccggg
      301 cagggcagaa agcatcagcg ctttccgcca cgggtgagag acataatttc atccgtgctc
      361 atacggacat cggatgtacc tcgaaggagt tccactgctc tgttccctct ggaagcgggg
      421 tttgcagcgc ggcgggcttt gcggatgaga gcgtagtctc ctgcgatttc aaattcgact
      481 tctgtgtgcg gcaaaagccc tagacgattg cggacctcct ggggaatggt cacctggcct
      541 ttgcttgtaa tctgcatggt attactctac caggtaagag tcataccatg caagttcatg
      601 cctgggcccg gtacagccat ctttcgcaat accgggacat gtgcgaggag agtggaccat
      661 ccccgggggc ccgtttccga gtgggcaggc tgtttgctta gctccggaat gtggtagcat
      721 cgtcaatctg taagacgccc cgttcttttc gggcgcctgc tggcctatgg accagaaggg
      781 cagcagatac ggtcatgaga cggcacggac gatgccggcg cgacatccct gtttcaaaag
      841 agcgggtccg gaggcgcttc gtgcgcgcac ggatgtccgc atcctgtttg cgaagcactc
      901 ccgcgcttct ccctgatgga gtgcgtgtct ttgcgtcgtg ttccggaagc ccgcggcatc
      961 aggcagggcc tttccggccg ggttttcagc caacaacaca aggagtcctc tatgaagtct
     1021 tatcagggaa gtgagattcg caacgtagct gtagtggggc atgcgcacag cggcaagacc
     1081 acgctcgtct ccgctctgct gcatgccgca aagatgacct caacaccgga acctgtgacc
     1141 gcctacgacg aagaagatgt tgcgcgtggc acgacgatgc tgaacgctgt ggcctatgcg
     1201 gagtggagcg gcgtgaaggt gaatttcata gatactcccg gatttcatat gtttgcgcat
     1261 gaggcgcgct gcgcgatggt cccggtggag tcggcgctgg tggtggtgaa cgctgccaat
     1321 gggcccgagg cagttaccga ccgcgtgtgg agttatgccg atgagttcaa tctgccgcgc
     1381 gtagcggtga tcaaccagat ggaccatccg cgggcggagt ggcagaaatc gctggagacg
     1441 atgcgcgagc ggtatggaag gcaccttgtg ccagtgcaat tgcctgtggt ggatgcggaa
     1501 ggatttcacg gcattgtgga cctggtgacg atggaggcat tcttctatac gccgaatggc
     1561 gatggtcacg gcaagatagc ggacattccg gcgaacatgg cagaggaggc gcagaaggcg
     1621 catgaggcgc tggtagagct tgtggctgaa ggcaaggacg aattgatgga agagttcttt
     1681 gccgagggta cgattccgga acaacacctg attacggcgc tgcatgaggc cattcgcgaa
     1741 gaccgcatct tcccggtgct ttttgcgagc gggggaacga atgttgctag cgaccatctg
     1801 ctggattttc tgaaggtcta tgcgccggcc ccggtagaac gcgagccggt ggcagcgcgc
     1861 gcgatgctgc aggccgtggc tgcccatgcg aatggcggcg gagacgagag caatccggag
     1921 atcgtgatgc ggcgcgtgga tgatggcgag ccgctggcgc tgtatgtttt caagacgatg
     1981 agcgatccgt ttgccgggcg catttcattc ttcaaggtct tctccgggca cgtgcggaat
     2041 gatgcagttg tggtgaacta ttcgcgcaaa gggacagaaa agttttccca tctgtccatc
     2101 atgcagggac gcacgctggt ccccgtgggg gagctgcatg cgggcgatct tggcgcggtt
     2161 gcgaagctga aagacacatt cacgggcgac acgctgggcg agaaagggca tgagattttc
     2221 tttgagccgg tgagtctgcc cgagccggcg atgacatatg cgattgagcc aaagtcgcgc
     2281 gcagatgaag acaagctggc cccggcgatg cacaagctga tggaggaaga cctgctggtg
     2341 cgcttctatc gcgatgcgca gacgaacgag tttctgattg ccggagcagg acagccgcat
     2401 atcgagtcgc tggtatcgaa gctgcgcaag cggtatcaca cggaggtgac attgaagtcg
     2461 ccgaaggtgc cgtacaggga gacgattcgc gggcgcgcgg aggcacaggg acgtcacaaa
     2521 aaacagaccg gagggcacgg gcagtttggc gattgcaaga tcagaatgga gccgctgccg
     2581 cgtggggccg gctttgaatt tgtgaatgac atttttggcg gcgcgattcc gcggcagttt
     2641 attcctgcgg tggagaaagg gattcaggaa gcggcagcgc gagggtatct ggcggggtat
     2701 ccggtggtgg acttcaaggt cacgctttat gacggaagct atcacgaggt ggattccaac
     2761 gaactgtcat tcaagcttgc ggggcggctg gccttccgta agtgcatgga gcaggccaag
     2821 cctgttctgc tggagccaat tatgcgggtg gaaatcgagg caccggagtc ctgcgcgggc
     2881 acgctgatgg gcgacctgaa ccaacggcga ggccgcgtgc agggcatgga gagccgtggg
     2941 gcaacgacgg tgattcgcgc cgaagtgccc atggcagaga tgctgacgta tgggcagagt
     3001 ctgacttcca tgacgcaggg gcaggggacg ttccacatgg agatggagca ttacgacatt
     3061 gttccgcaac cgctggcaga gaagatcatc gccaatgcga agaggccgca ggaggaagaa
     3121 gaggagtgag gggtgcagtg ccctggtttc cgccgttttc cggtctgact tcaggccgga
     3181 aacgggggat gcgcgaaata gcgcggtgaa gcctgaggtt ttatggacat cccggctgat
     3241 ttcgctgatt attgggtgtg gagagaaaac aggatgacga ttgctgaagt tctgcttgcc
     3301 gattttgaca gcgaaatagg gaatacacgc cgcacgctgg agcgcgtccc cgaggacagg
     3361 ccggattaca agccgcatga gaaatccatg ccgctgggga ggctggcgat gcattgcgcc
     3421 acgcttcctc tttttggcta ttacattatg gaagacgacg cgatggacct tgccgcgccg
     3481 aagcgtcctc acgctgacct taccttcagg tcacgcgcgg aatgtctggc ccaactggat
     3541 gaaactgctg cgaagtgccg cgcggcgctg gcttcggcaa gcgatgaaca ttttcagaag
     3601 aagtggcctt tcacctgggg tgaacacgtc atctcaaacg aaacgcgcct gcgcacatac
     3661 cgcacgatgt tttttaacca catgattcac catgttgcgc aactgggcgt ttatctacgt
     3721 ctgaatcatc ttgcggtgcc ggctttgtat ggtccttcgg cggacgaaca atggtctccg
     3781 aagtagccct gttctgatta tcggaccgcc ccgacagcgt acaaaacgct ggcatggggc
     3841 ggaagggtga ccttcaaccc tggcatctgg cccaggtctt ttcgttgcca cagatcacga
     3901 acgtggcccg gggccgtgat ttcaagctct ttccaggaaa aggaaatgtt ctgtggcgtg
     3961 tcgcccaggt taaaggcggc cacgtactgc tttcctgcgg caccctttgc ggtccagacg
     4021 acggtgtccg gcgtctgaat tacgggcttg tttgcgctgg aatgctggtc tacagcgatg
     4081 acttcaggat tggtcaggag cgattcggtg aagctgtcca tttgcgtgag atttgctccg
     4141 aggacgagcg gggagcgcgc aatggaccac agggtcacga gggtgcgcgc ttcatcttga
     4201 gagaggcggg aatggcgtgg ctggccccat ccggcattgg ggccgagata gccgaggggc
     4261 agcatgtcgg cgtctggcca gtggcctggt tcgatgtgtg aagaccagtc ggccagattt
     4321 ttgaactgcc gcctgagagg ttgcgggaag gtgttggtat cgctttccgg cttgttccag
     4381 aggtcccaga catcgttgga gatgcgccac atctgcgcgt atttccggac gtccccggct
     4441 tctgagatgg gtgttggtcc gggcgagagg ctcagcacga tggggcggcc gctgtttttc
     4501 aagcctgcgc tcatcatgtg gatttcgtct gctttatagg gcgaggagat gcagtccact
     4561 ttcaggaaat cgaggcccca gccggcatag agttttgcaa tcgaattgta ataggcctga
     4621 gcagcggcgt tgtttttgag gccatagttg tccggattcc attggcaaag gtctgaggtg
     4681 ttggccgcgt cggcagcatg gaaccgggaa ccggcgatgg gcagattttt ctcgacggcc
     4741 tctttgggga taccgcgaat gatgtggatg ccgaacttga ggccgagcga gtgcacataa
     4801 tccgctatcg gtttcagtcc tgcgcccttg acgcttgagg ggaagcggtc cggagctggt
     4861 acgtaacggc cgtagctgtc gagagtgtat ccctggtcgg cgccctgttt gccagcgttc
     4921 tccggatgtt gcaggtacca gccttcgtca atgacgacat atttccagcc gtactgtttc
     4981 aggtgctgat tcagccactg gacgttggct ttgaactggg cttccgtgat ggtgaggccg
     5041 taggagtccc aactgttcca gcccatgggc ggagtggggg cgagcagcgg agttctggcc
     5101 tgagcgggaa ggagcagagc ggcactgaga aggagcaggg aaacaagcag gaagcgtttc
     5161 atcatcacct tttgcagaga acgttttctg tggcaaaaga aggataacag catggtgaat
     5221 taaatcacca cgctttcctg atactcgccg aaaatctcgc gcatggctgc ggcgatttct
     5281 cctacggtgg catggctttc gactgcttca agaattgcgg gcatcaggtt ttgcccggag
     5341 cgggcgtggt ttttcacgcg ctccagcgat gactgccagc gggccttgtc tctgcgctgg
     5401 cgcagagcgc ggacgcgctc cacctgtctg cgctccagat cttcatctat gcgctggatg
     5461 ggaatgggcg gcccgtcttc gcgcgtgaag cggttgacgc cgactacgac ggcctggcct
     5521 gcatctaccg cgcgttggta atcataagcg gagttctgaa tttcctgctg cacccatccc
     5581 tgctcgatgg cgcgcaacat gccgccgagt gcggcgatgc ggtcgaggta ggtcttcacc
     5641 tgttgttcaa gcttttgtgt gagcgcttcg atgtagtaag agccggcgaa ggggtctatg
     5701 gtgtgcgcca caccggtttc acaggcaatg atctgctgcg tgcgcagcgc gatacgcgcg
     5761 gcttcttcgg tgggcagagc gagggcctcg tcgtatccat tggtatgcag cgattgcgtg
     5821 ccgccgagca cggctgaaag cgcctgaatg gcggtgcgga cgatgttatt ctccggctgc
     5881 tgcgcggtga gcgtagagcc ggcggtctgc gtatggaagc gcagcatcca ggagcgcgga
     5941 tgtttggcgc cgaagtggct gcgcatgatg tgcgcccata cgcggcgcgc gcagcggaat
     6001 tttgccactt cttccaggaa attgttgtgc gcattgaaga aaaaggagag gcgcggggcg
     6061 aatttgtcaa catcaagccc ggcgtctatt gcggcctgga cgtaggtcat tccgttggcc
     6121 agggtaaagg cgacttcctg ggcagcggtc gaaccggctt cgcgcatgtg gtatccggag
     6181 atggagatgg tgttccattc gggcaattct tcgttggccc acgcgaacat gtcggtaata
     6241 atacgcatgg cgtgatggat ggggtagatg taggtcccgc gggcaatgta ttccttaagg
     6301 atgtcgttct gcacggtgcc ggagagcttt tttacgttgg ccccggtgcg gcgggccacg
     6361 gcgacgtaca gcgccagcag aatgctcgcc gtggcgttga tggtcatgga agtggagatt
     6421 ctgtcgaggg ggatggagtc gaagagccgc tccatgtctt cgatggaatc aatggctacg
     6481 ccgacctttc ctacttcgcc cagggacatg gggtggtcag agtcatagcc gatctgggta
     6541 ggaagatcaa aggcgacgga gaggcccgct gtgccctgtt gcaggagaaa tttgtagcgg
     6601 cggttgctct cttcggcgtc gcccatacct gcgtactgac gcatggtcca gagccggccg
     6661 cggtacatcg tgggctggat gccgcgggtg aagggaaatt cgccagggaa acccaggtcg
     6721 cggagcgggt cgaagccgat ggattcgagg tcttcgcggg tataaacgga tttctgctcg
     6781 gcagacatgg tgaacgaaga aggataacag gggagcgggg cgcaggtctc agggacggca
     6841 gttgaaatct acggcatttt cacagattcc gtacactgca tttgcactgt gccgtgtcgc
     6901 cgactccctg ttggtcgcgg cgactcaagt ttcgtggcat ggtgcagact atctgtgaaa
     6961 atgcgctagc gcctgtgacc tgagcgctgt tttctgcctg cgcgataaaa cgagctgccg
     7021 gcgaaccagg caaagacgag ggtcactgca gcgaaggcga ggaagcgcgc gtgtccaggc
     7081 ccggcggacc agttctggcg aagtttccag gtattggagg caaaaacgat ggcgaagacg
     7141 gagaaaaaaa gcccggtaat ctgaagccag atgataccgc tggcatgggc gacg
//
LOCUS       DASRBV010000204         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_417578, whole genome shotgun sequence.
ACCESSION   DASRBV010000204 DASRBV010000000
VERSION     DASRBV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417578"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFA02_07805"
     CDS             <1..852
                     /locus_tag="VFA02_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZU09794.1"
                     /translation="FLELFTTIIVAYIFVLLGVVHERVALTMVYLDILLYSAGGVIGT
                     MHHLYFSGEPAVHMALGAFFSAAEVIPLTFLTLEAWSFLQLGAEQSARSKSPFPHFWA
                     VMFLAAVGFWNFLGAGIFGFLINLPVVSYFEIGTALTANHAHAAMMGVYGMLAVGLSL
                     FCLRYMVPQARWSDRAAKVSFWSLNLGLAWMVFASLFPLGIIQLYHSVRFGYYDARTL
                     EYLTGHLNALIEWMRLPGDALFIVGGVLPLLYLEWLGARHVAPKAAAEQPAESLFTEI
                     IESAGVK"
     gene            852..1400
                     /locus_tag="VFA02_07810"
     CDS             852..1400
                     /locus_tag="VFA02_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09795.1"
                     /translation="MIDGFHAELLMAAGYAVFLMAAAAILEWMAKHSHRRSEQMRVTG
                     FRFHREFDRWECPTGQLLRRHSVDHERRIVRYRAPAHVCNCCHKKPDCTDSDEGREIE
                     HRVDSWVQSEIRRFHRGISLALLFLAGLLLAAQIVWYKAPRELALTGGLLLAIGAAGA
                     RLLAGFSSIRSADTSFRDARPQ"
     gene            2379..>2985
                     /locus_tag="VFA02_07815"
     CDS             2379..>2985
                     /locus_tag="VFA02_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09796.1"
                     /translation="MGLQGTKLNRWVVSALAVLAPVFYVGCQNKADPLPSQTAPIVEV
                     ATVIQKDVPVYGEWVGTVQGYLDARISPQVSGYLVRQDYREGGIVQKGQVLFEIDPRP
                     YQAALDQAKAQLAEAEAQLAKATLDVHRDVPEAQAHAIPLSQLDNDRQAQLAAQASVE
                     AGKAAVEQAELNLGYTRVRSLIPGIAGIATVQVGNLVGPSTV"
BASE COUNT          556 a          860 c          920 g          649 t
ORIGIN      
        1 tttcttgaac ttttcaccac gatcatcgtc gcttacatct tcgtgctgct gggcgtcgtt
       61 catgagcgcg tagcactgac gatggtttat ctcgacattc tgctctattc ggccggcggc
      121 gtgatcggca ccatgcatca tctctacttc tccggcgagc ccgccgtgca catggcgctt
      181 ggagctttct tctccgccgc ggaagttatc ccgctgacgt tcctgacgct tgaggcatgg
      241 agcttcctgc agttgggcgc ggagcagagt gcaaggtcga aatcgccgtt cccgcacttc
      301 tgggccgtaa tgttccttgc ggcggtcggt ttctggaact ttctcggcgc tggaatcttc
      361 ggcttcctca tcaatcttcc cgttgtttcc tatttcgaaa tagggacggc actgacggcc
      421 aatcacgctc acgctgcgat gatgggcgtt tacggaatgc tggccgtcgg gctgtcgctc
      481 ttctgcctgc gttacatggt tccacaggcc cgctggtctg accgcgcggc gaaggtcagc
      541 ttctggtcgc tgaatctggg gctcgcgtgg atggtctttg cttcgctgtt ccctcttggc
      601 atcatccagt tgtaccactc cgttcgcttc ggctattacg acgcgcggac gctcgaatac
      661 ctcaccggcc atctgaacgc cttgattgaa tggatgcgcc tgccgggcga cgcgctgttt
      721 atcgtgggag gcgttctgcc gctcctctat cttgaatggc tgggagcacg gcatgtggcc
      781 ccgaaagctg cggccgagca gccggcggaa agcctcttta ctgaaatcat cgaatccgcc
      841 ggggtgaagt gatgatcgat ggcttccatg cggagttgct gatggcggcg ggatacgcag
      901 tgttcctgat ggctgcggcg gccatccttg aatggatggc aaagcattcc catcgccggt
      961 ccgagcagat gcgcgtgacc gggttcaggt tccacagaga attcgaccgc tgggaatgcc
     1021 caacgggaca gttgctccgg cggcattcgg ttgaccatga gcgccgcatt gtgcgttacc
     1081 gcgcacccgc gcacgtctgc aactgctgcc acaaaaagcc tgactgcaca gattccgacg
     1141 aaggccggga gatcgagcac cgcgttgatt cctgggtgca gtcggaaatc cgccgctttc
     1201 atcgcggcat ctccctggcg ctgctgttcc tggctgggct gcttctggcc gcacaaatcg
     1261 tgtggtacaa ggcgccgcgc gaactggcgt tgactggcgg actcctgctc gccatcgggg
     1321 cggccggcgc acgactgctg gccgggttca gctctatacg atcggctgac acgagcttcc
     1381 gcgacgcgcg cccgcaataa gtacaccgtc tcctcattgg acggaatggc aaagatcagg
     1441 ccggtgcggg acgaggcgat gacagagttg ctgaatgatc ggcgaagggg tggcgccgac
     1501 atagcctgac aattctacgc cagcccggcg gctgccttgc tctccccagc aagggtgtca
     1561 tctccttcct cctctttcga cttggaccat tcaacctcgc gcgggaaagg gtggactctt
     1621 ccaacgccac aaatgcaatg ggccattgac agcaggcgga gactgtcaca taattgtcga
     1681 atgcaacaat tgcatcatgc aatgactgca gccaccccgc gtggttgtgc ggcagaaatt
     1741 ctggaagttg tgccggccgt gatgcggttc attcgcaccc agatgcggca gtaccgaggt
     1801 cctgaccttt ccgtaccgca gtttcgcact ttggtattcc tctgcagcag cccgggagcg
     1861 tctctttccg tactggcgga cttcctcggg ctttccctgc ctgcaacttc ccgactggtt
     1921 gaaggattgg tacggagaaa cttagtggac cgccgcattc catcgggaaa ccggagactg
     1981 gtttctctga cagtgagcgc acacgggcgg aagatcatat gcgcggcgcg aaaagccact
     2041 gaaaggcggc ttgcagacgt gatgtccgcg ttgccgacca aagactgcgt ggcaatccag
     2101 cgcgcactcc aggcgctgcg aaagagattc tgcccttagc gatcatgagg gttttagaaa
     2161 gtgtctgcga aacgctatca gcgtgctgcc gctgctgcgg cgacggaaca gttgccatcg
     2221 agggcttgcg cggaatggac ggcagagtct tcggtctgtc ggggccggcg ggtgtgaaca
     2281 ggaaagaacg tggggcccgg cgaggcctga gttcgaatgg agattcaggg cttgctggcg
     2341 ttggcattta tcgcgggcac actgaggagt aatagccatt ggggttgcaa ggaacgaaac
     2401 tgaacagatg ggtggtaagc gcgttggccg tgctcgcgcc ggttttctat gtcggctgcc
     2461 aaaacaaggc ggatccattg ccatcgcaga ccgctccgat tgttgaagtc gcaaccgtca
     2521 tccagaaaga tgtgccggtt tacggcgagt gggtgggcac ggttcaaggc taccttgatg
     2581 ccaggatctc tccgcaggtg agcggatacc tcgtccgaca ggattaccgc gaaggcggga
     2641 tcgtccagaa gggccaggtg cttttcgaga tcgatccccg gccttatcag gctgcgctcg
     2701 accaggcaaa agcgcagttg gcggaagctg aagcgcaact ggcaaaagcc acgctcgacg
     2761 tccatcgcga tgttcctgag gcgcaggctc acgcgatccc cctcagtcag ctcgacaacg
     2821 accggcaggc gcaactggca gcgcaagcgt ccgtcgaggc gggcaaggct gctgttgagc
     2881 aggcagaact caatcttggg tatacaagag tccggtctct gattcctgga atcgcgggaa
     2941 ttgcgaccgt gcaggttgga aatctggtcg gcccttccac cgtgc
//
LOCUS       DASRBV010000205         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_112952, whole genome shotgun sequence.
ACCESSION   DASRBV010000205 DASRBV010000000
VERSION     DASRBV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112952"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFA02_07820"
     CDS             <1..1034
                     /locus_tag="VFA02_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HZU09797.1"
                     /translation="SMADTYKIAIVGAASLRGKELNEALGDSAFGASDFVLMDDESSL
                     GQLEAVGDEVTFIQRIESDAFNNIDFVFFAGAEDVTRKHWLKAQRAGASIIDMSYALE
                     SEPGVLVRAPWVQESKTAPDLKTTAVSSAHPAAVVLALVLKRVQTVANVVSASATIME
                     PASEYGRTALDELHQQTVGLLSFQPLPKDLYDTQVAFNVVSGLGESAKISLIASEARI
                     RRHYEQLAEGRLPEAMIQLLHVPVFHGYGLSLAVTLDEPVSLDHIETSLSGDHMDVIV
                     SGSEAPSNLSSAGQSDVLARVRTADGTELATNKFWIWIVADNLKLAALNALACAEELR
                     RLRPQGKVQ"
     gene            complement(1080..>2094)
                     /gene="mtnA"
                     /locus_tag="VFA02_07825"
     CDS             complement(1080..>2094)
                     /gene="mtnA"
                     /locus_tag="VFA02_07825"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZU09798.1"
                     /translation="FLDQTKLPLEETYVLATSYQQVADVITTMVVRGAPAIGVSAAMG
                     VALGVKQSKAVNLAALKTEFETIAKTLATTRPTAVNLFWAIERMRTCFTALVEQPGAT
                     LESISEALITEAQRMYEEDIAACKAMGANGAELLPKEGGVLTHCNAGALATCGYGTAL
                     GVIRAAVERGHNVHVYADETRPFLQGARLTAWELMHDGIPTTVICDNMAASLMRQGKI
                     RAVVVGADRIAANGDVANKIGTYNVAILAKEHGIPFYVAAPWSTIDLATPSGDLIPIE
                     QRPAIEVTHHAGKQLTPHGVEIENPAFDVTPAKYVTAIITERGVLRAPYAESLAGLEA
                     QPA"
BASE COUNT          426 a          633 c          579 g          456 t
ORIGIN      
        1 actccatggc tgatacatat aagatcgcaa tcgtaggcgc agcctcactg cgcggcaaag
       61 aactgaatga agcgctgggc gattccgcgt tcggcgcatc cgattttgtg ctgatggacg
      121 acgagtcatc cctggggcag cttgaggccg tcggggatga agtcactttt attcagcgca
      181 tcgaatctga tgctttcaac aatattgatt ttgtcttttt tgcgggagcg gaagatgtca
      241 cccgcaagca ttggctcaaa gcgcagagag caggcgccag cattattgac atgagctatg
      301 cgctcgaaag tgagccgggg gtcctggtgc gcgccccctg ggtgcaggaa tcaaagaccg
      361 cgccggacct gaagaccacg gcggtttcct ccgcgcatcc tgcggccgtt gtcctcgcgc
      421 tggtgctgaa acgcgtgcag acagtcgcaa atgtggtctc cgcttctgca accattatgg
      481 agcctgcctc agaatatgga cgtactgccc tggatgagct gcatcaacag accgtgggtc
      541 tgctgagctt ccagcctctt cccaaagacc tttacgatac gcaagtcgcc ttcaacgtgg
      601 tctcaggatt aggtgaatca gccaagatca gcctgatcgc aagcgaggcg cgcatccgcc
      661 gccactacga acaactggca gaaggccgcc tgccggaagc catgatccag cttctgcacg
      721 ttcccgtctt tcacggatac ggcctctcgc tcgcagttac tctggacgag cctgtatctc
      781 tcgatcacat cgaaacgtcc ttatcaggcg accacatgga tgtcatcgtg agcggctctg
      841 aagcgcccag taacctcagc tccgctgggc aaagcgatgt tcttgcgcgc gtgcgcaccg
      901 ccgacggcac cgaactggcg acaaataaat tctggatctg gatcgttgct gacaatctga
      961 agctggctgc tttgaacgct ttggcctgcg cggaggaact gcggcgcctt cgcccacagg
     1021 gaaaagtcca atgacgccgg cctcccactc aggcttcagt ctgagtggga agtcaggttt
     1081 caggctggct gcgcttcaag cccagccagc gattcagcat agggtgcccg cagcactccg
     1141 cgctctgtaa taatcgctgt tacatatttc gccggagtca catcgaacgc cgggttctcg
     1201 atttccactc cgtgcggcgt aagttgtttt cctgcatgat gggtcacctc aatcgcaggg
     1261 cgctgctcaa tcggaatcag gtcgccggaa ggggtcgcaa ggtcaatcgt agaccacggg
     1321 gccgccacat aaaacggaat tccgtgctcc ttcgccagaa tggccacgtt atatgtgccg
     1381 atcttgttgg ccacgtctcc attggccgca atgcggtctg cgcctacaac cacggcgcga
     1441 atcttgccct gtctcatcag gctggcagcc atgttgtcgc aaatgactgt agtggggatg
     1501 ccatcgtgca tcagctccca ggctgtcagg cgggcgcctt gcaggaacgg gcgcgtctcg
     1561 tccgcataga catggacatt gtgcccacgc tccactgcgg cccggatcac gccaagcgct
     1621 gtgccgtatc cgcacgtggc cagagcacct gcattgcagt gtgtcaacac tccgccttcc
     1681 ttaggcagca gttccgcgcc gttcgctccc atggccttgc aggcggcaat gtcttcctca
     1741 tacatcctct gtgcctctgt aattaacgcc tcggaaatgg attcaagagt ggccccaggc
     1801 tgttccacaa gcgcagtaaa acacgtcctc atgcgttcaa tggcccagaa gaggttcacc
     1861 gccgtgggac gggtcgttgc cagcgtcttc gcaatcgttt caaattcagt tttcagcgca
     1921 gcgagattta cggccttgct ctgctttacg cccagggcca cacccatggc cgcggaaacg
     1981 ccaatcgccg gagcgccgcg cactaccatc gtggtgatca catcggccac ttgctgatag
     2041 ctggttgcca gcacatacgt ctcttccagc ggcagtttgg tctgatcaag aaag
//
LOCUS       DASRBV010000206         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_521205, whole genome shotgun sequence.
ACCESSION   DASRBV010000206 DASRBV010000000
VERSION     DASRBV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521205"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFA02_07830"
     CDS             <1..416
                     /locus_tag="VFA02_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZU09799.1"
                     /translation="ENMDDVQRSMGMLLNNTPWHAPVTEKPVLNSTEIWELVNLTDDS
                     HPIHLHLVRFQVLDRRRFDSFHFQQTGEMRFTGPAVPPDANEMGWKDTVRCDPNMVTR
                     IIIPFVGYIGRYVWHCHVLEHEDNEMMRPFEVVPD"
     gene            complement(504..1757)
                     /locus_tag="VFA02_07835"
     CDS             complement(504..1757)
                     /locus_tag="VFA02_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZU09800.1"
                     /translation="MTLLLVQMSVILAVTLVCGWLARRLGQARVIGEMIGGILLGPSV
                     LGRISPHASTALFPAGSLHFLEILSTVGLVLYLFLIGMELDYDQLVRQRRTALFASGT
                     SIIIPFITAAVLAHSLRIRFAPHGIGSVPFVLFLGIAMSITAFPVLARILEERRLQGT
                     SLGTTAILCAAVDDVVAWSLLALALALIGADGGSASLPIRLACLAGYLLFMLGIVRPL
                     ATRAVHMRKKPGLSLELLGVFIIGALASAAVTEWIGVHPLFGAFLAGVCFPRIEHWQH
                     AIRARLDMVTSVLLLPLFFALTGLRTRLDLLNDAKMWWWAGIVLIGAVFGKMGGGVLA
                     ARWTGQSWRNAVALGTLMNTRGLVELVVLNIAYNVGAFSPTLFTMMVVMALVTTMITT
                     PILNLLGIKSEEAEPHNIEVEVTAA"
     gene            1873..2886
                     /locus_tag="VFA02_07840"
     CDS             1873..2886
                     /locus_tag="VFA02_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HZU09801.1"
                     /translation="MDNRTLPDIFDVLVIGAGPTGMACAIEAQRVGLRSALVDKGCLC
                     NSIYHYPSNMTFFTTPELLEIGDIPFPSVNQKPTRNEALEYYRKVAEHYQLDVRQYQN
                     VDSVSGSDGDFRVHVTDQFGRKFIHHTRKLVVATGYYDLPNYLGIPGEDLPKVHHYYE
                     DPHPYYDLDVLVIGGKNSAAIAALDLWRHGARVTLVHRGTGMHRHVKYWILPDIENRI
                     KNGEITAYFESEVERIEEDSVTLKTPQGEVVLENDFVFAMTGYHPDFDFLTALGIRLE
                     SKHRCPECDPQSLESNVPGIYLAGVIIAGERTNEIFIENGRFHGRQIAADLKEKFSAA
                     LKA"
     gene            2999..4837
                     /locus_tag="VFA02_07845"
     CDS             2999..4837
                     /locus_tag="VFA02_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU09802.1"
                     /translation="MLFELLALALFTAFLVREQRKELQQRIDRRLEYQGSQFATQAAS
                     AIEEGKTESLQKMVSAMLRYPSIGSVQITDIQGVAIAGADISMNGKLNLTATERNYLR
                     DLDKPTIFSLKRNVREIVAPVIVRGQVQALVWISPNEEADRLQLHSLLRVTLISAILA
                     TLGCTILATLMARTVTRPLNVLMRATRQIVRDPEDVSNFPLPVTSENEAADLTTAFNM
                     MVASIEEQRSGLNDTLALLDSMLANAPIGFAFFDRKFRFVRVNQFLADMNRSTISRHL
                     GRAVNEVFPESAAVPLQRAIEQVFSTGRPVQDLELVGEVEGQPGRVRSWLANIYPVQT
                     GTNAVRWVGTVMVDTTQRRSSEEALRKTEKLAAAGRLAASIAHEINNPLEAVTNLLYL
                     IRLQEKLPSEVEKYVELAQHELARVSEIAQQTLRFYRQSTLPITANISELLDSVLTLH
                     QGRVNSLQIKVRKFYDSDVEVFCFAGEMRQLFANLISNALDAMMPGPGTLTLSVRRSH
                     SWRDPSIRGVRISVADTGCGMPDDVRTRIFEPFFTTKEATGTGLGLWVSAEVIQKHRG
                     EIRIRSRVMNDNHPGRQPGTIFMIFFPQSAVEEAISESLETSSQTV"
BASE COUNT         1124 a         1273 c         1397 g         1043 t
ORIGIN      
        1 gcgagaacat ggacgatgtg cagcgctcca tgggcatgct gctgaacaat acgccctggc
       61 atgctccggt tacagagaag ccggtgctga attcgaccga aatctgggag ctggtgaacc
      121 tgacggatga ttcgcacccg atccatcttc atctggttcg ctttcaggtc ctcgacaggc
      181 gacgcttcga ttcgttccat ttccagcaga caggcgagat gcggtttact ggtccggctg
      241 ttccacccga tgcgaacgaa atgggatgga aggacacagt gcgctgtgat ccgaatatgg
      301 taacgcgcat catcatccca ttcgttggat acatagggcg ttacgtctgg cattgccatg
      361 tgctggagca cgaggacaat gagatgatgc ggccctttga ggttgtgccg gactgagtta
      421 ccgcctgcag cggcccatgc cgaagatgct tccggtcatg tctgtggggc cgtagctgcc
      481 tgggtacggc caggcatgat ggttcaggct gcggtgacct ctacctctat gttgtggggt
      541 tcagcctctt cgcttttaat tcccaacaga ttcaggatgg gcgttgtaat cattgtggtg
      601 accagggcca tgactaccat catggtgaag agtgtgggtg agaatgctcc tacgttatag
      661 gcaatattca ggacgacgag ttctacgagg ccgcgggtgt tcatcagtgt gccgagcgcg
      721 acggcattgc gccaggactg ccccgtccag cgtgccgcaa gaacacctcc acccattttt
      781 ccaaatacag cgcctatgag gacgatgcca gcccaccacc acatctttgc atcattcaac
      841 aggtccaggc gtgtgcgcag gcctgttaat gcaaagaaca gcggcaggag caggacagaa
      901 gtgaccatat cgagacgggc gcggatggca tgttgccagt gctcaatgcg tggaaagcag
      961 acaccagcga gaaatgctcc gaagagagga tgcacgccga tccattcggt aactgcggcg
     1021 gaagccagtg cgccgatgat aaaaacgccc agcagctcca gtgaaagccc gggctttttg
     1081 cgcatgtgga cagcgcgcgt ggccagtggc cggacaattc caagcatgaa cagcagatat
     1141 cctgccagac aggccaggcg aatgggtaat gaagccgagc ctccatcggc gccaatgagt
     1201 gcaagcgcga gcgccagcaa tgaccatgcc accacgtcat ctacggccgc gcaaagaatc
     1261 gccgtggtac cgagagaagt gccttgcagg cgccgttctt ccagaatgcg cgccagtacc
     1321 ggaaacgcgg tgatgctcat ggcgatccct aaaaagagta caaagggaac gctgccgatt
     1381 ccgtgcggag caaaccggat acgcagcgaa tgtgcgagga ccgcggctgt aataaatgga
     1441 ataatgatgc tggtgccgct ggcaaacagc gccgttctgc gctgacggac caactggtcg
     1501 taatccaact ccattccgat gagaaagaga tacaggacca ggccgacggt cgaaagtatt
     1561 tccaggaaat gcagagaacc ggcagggaag agcgctgtcg aagcatgggg cgagatgcgg
     1621 cccaggacgg aaggaccgag tagaatccca ccgatcattt cgccgatgac gcgcgcttgt
     1681 ccgagtctgc gggccagcca tccgcaaacc agcgtaacgg cgaggatgac ggacatctgc
     1741 acgaggagta gggtcatacg gccttcaatc ctgagttctc cgggaaattt ttaaatgaga
     1801 tgccggtttt gctttccgcg tttgatgcct gccgggaaga actttcaggg tgctatcgtc
     1861 taatctaaag gcatggataa tcgcactttg cctgatatct tcgatgttct ggtgattggg
     1921 gccgggccga caggcatggc ctgcgccatt gaggcgcagc gcgtgggcct gcgcagtgcc
     1981 ctggtagata aaggctgctt gtgcaattcc atttatcact acccctcgaa catgacgttc
     2041 tttaccactc cggagctgct ggagattggc gacatcccct ttccaagcgt gaaccagaag
     2101 cccacccgca atgaggccct ggaatactac cggaaagttg ccgaacatta tcagcttgat
     2161 gtgcgccagt accagaatgt agactccgtt tccggttcgg atggggactt tcgtgtccat
     2221 gtaaccgacc agttcgggcg gaaatttatc caccatacgc gaaagctggt agtggcgacc
     2281 ggatactatg acctgccgaa ttatttggga attcccggcg aggacctgcc gaaagtccac
     2341 cactattatg aagatccaca tccttattac gatctggatg tgcttgtaat tggcggcaag
     2401 aactcggcag cgattgcagc actggacctg tggcggcacg gggcgagagt gacgctggtg
     2461 catcgcggca ccggaatgca caggcacgta aagtactgga ttcttcccga tatcgaaaat
     2521 cgcatcaaaa atggagagat tactgcatat tttgagagcg aggtggagcg cattgaagag
     2581 gattcagtga ccctgaaaac accgcagggc gaagtcgtgc tggagaacga cttcgtattt
     2641 gccatgacag ggtaccatcc ggatttcgat tttcttacag cgctggggat tcgcctggaa
     2701 agcaaacacc gctgcccgga atgcgatccg caaagtctgg aaagcaatgt gcccgggatt
     2761 tatcttgctg gcgtgattat tgccggagag cggacgaatg agatttttat agagaatggc
     2821 cgttttcatg gcaggcagat tgcagccgac cttaaagaga aattttctgc cgctctgaag
     2881 gcgtaagctg ttagatggtc ttttctgcgc ggagattccg tgcgtgcgcg catttttgta
     2941 ggacggaagc attttgctca aaccacggac aattcggggc caattaatcg ccagtctcgt
     3001 gctattcgaa ctgctggcgc tggcgctctt cactgcattt ctggtacgcg aacagcgaaa
     3061 agagctacag cagcggattg accgccgtct ggaataccaa gggtcccaat ttgccacaca
     3121 agcagccagc gcaattgaag aaggcaaaac ggaatccttg cagaagatgg tcagcgccat
     3181 gcttcggtat ccgagcattg gcagcgtgca gattacggac atccaaggcg tggccattgc
     3241 cggtgcggac atttcgatga atggcaagct caaccttact gcaacggaac gcaattatct
     3301 ccgggacctc gacaagccaa ccatcttttc gctgaaaagg aacgtgcggg agattgttgc
     3361 tcctgtgata gtgcgtggcc aggtgcaggc gttggtgtgg atttctccca atgaagaagc
     3421 cgaccgcttg caattgcact cgttgctgcg ggtcacgttg atttccgcca ttcttgcaac
     3481 cctgggctgc acaattctgg ccacgttgat ggcacgtact gtgaccaggc cgttgaatgt
     3541 gctgatgcgc gccacgcgcc agattgtgcg cgacccggag gacgtctcca attttccgct
     3601 gccagttacc tcggaaaatg aagcggccga cctgacgacc gctttcaata tgatggtcgc
     3661 ctcgattgag gagcagcgtt ccggactgaa tgacacgctg gcgcttctgg attcgatgct
     3721 ggcaaatgcg cccatcggtt tcgcgttttt tgaccgcaag ttccgctttg tgcgtgtcaa
     3781 tcagttcctt gccgacatga accgcagcac catcagccgc catctgggac gtgctgtcaa
     3841 tgaggtcttt cctgaatcgg cggctgtgcc attacagcgc gcaatagaac aggtgtttag
     3901 cactggcaga ccggtgcagg acctggaact cgtcggtgag gtggagggcc agcccggacg
     3961 tgtccgctca tggctggcga acatctatcc ggtgcagacg ggaacgaatg ctgttcgatg
     4021 ggttggcact gtgatggtgg acaccacgca acgccgtagc tcagaagaag cactacggaa
     4081 aacagaaaaa cttgccgcgg ctggacggct tgccgcctcg attgcgcatg aaatcaacaa
     4141 tccactggaa gctgtcacaa acctgctcta tctgattcga cttcaggaaa agcttccatc
     4201 ggaagtggag aagtacgtcg agctggcgca acacgagctt gcgcgcgtct cagaaatcgc
     4261 acagcagaca ctgcgtttct accggcaatc caccttgccc attacggcga acatcagtga
     4321 actgctcgat tctgtcctga cgctgcatca ggggcgtgtg aactcgctcc agataaaggt
     4381 gcgcaaattt tacgattcgg atgtggaggt attttgcttt gcaggcgaga tgcgtcagct
     4441 tttcgccaac ctcatcagca atgcgcttga cgccatgatg ccgggaccgg gcaccctgac
     4501 actgagtgtc cggcgatctc attcatggcg tgatccttct atcaggggag tgcgcatctc
     4561 ggttgccgat accggatgcg gcatgccgga tgatgtcagg acgcgcatct ttgaaccctt
     4621 ctttactact aaagaagcca cgggtaccgg actggggctt tgggtgagcg ctgaagtcat
     4681 ccagaagcat cgcggtgaaa ttcgcattcg cagccgcgtc atgaatgata accaccccgg
     4741 acgccagccg ggaacgattt ttatgatctt cttcccacaa tcggcagtag aggaggccat
     4801 ttcagagagt cttgaaacca gttcccaaac cgtgtga
//
LOCUS       DASRBV010000207         9477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_218990, whole genome shotgun sequence.
ACCESSION   DASRBV010000207 DASRBV010000000
VERSION     DASRBV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218990"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFA02_07850"
     CDS             complement(<1..1065)
                     /locus_tag="VFA02_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU09803.1"
                     /translation="MRMTVILFTLFSLTASAQTSATATIVGTITDPTGSAVPNATITV
                     TDTATGITHSATSNGTGEYVLPALQIGTYAIKVEAAGFNVQTKTGVVLNVGDRARVNF
                     TLQVGAAQETVTVSASGIKLQTDSGEVSNVISGKQITQLESNGRSLYSLMNLTTGASS
                     LQMDFQVPTPMGGDSSVSFNGQRNSHNLFLLDGGETDDRGGGGSAIVMPSEDALAEFR
                     QLTSNYSAQYGLSSAATISTVIKSGTRNFHASGWWFGRNDAFNARNYFNPQYSADGTH
                     NKVAKLRFNLWGFNVGGPVALHGGGNPKTFFFYNMEWRSLIQGNNFNVGVPFTSTYGG
                     DLSQAINTGIVTSVRAPFDCQ"
     gene            1556..3772
                     /locus_tag="VFA02_07855"
     CDS             1556..3772
                     /locus_tag="VFA02_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU09804.1"
                     /translation="MLIHTLRKCLSLALLAACSIALAQNNPQDRPWMNTSLSPEKRAD
                     LVLKQMTLDEKIDLLHGNGMAHTGNWQMPLTHLSNGGAGYVVGVPRLGIPPLYISDAA
                     YGVRSSGENGRYSTALPSNLGAASSWNPQAACEYGALIGRELRAQGYNMTLGGGVNLT
                     REPRNGRTFEYMGEDPLLAGTMVGNLMKCEQGEHVVGDIKHYAMNDQETGRNIVNVIV
                     SKRAMRESDLLAFQIGLKISNAAAAMCSYNRVNGDYSCENKYLLTDVLKHDWDFKGFV
                     LSDWGGTHSTEKASAAGLDQEQPMADYFGPKLKEAVEAGRVPMSEVDDHARRVLWAEF
                     ASGIVDDPVQKSVVDAEGGLEIAQRLEEQSIVLLKNEHDILPLNPAKLHSLAVIGGHA
                     DVGMISGGGSAQVDPPGGNAIAPPGHKATTWQAHIWFPTSPLKALRAKLPNAKVEYNS
                     GEDLDAAAALAKDSDVAIVFAYQWESEGMDLPNLSLPDNQDALIERVAAANPHTIVVL
                     ETGTAVTMPWINKVSGIVEAWYAGSSGHRALANVLLGEVNPSAKLAMTFPKSEQDLPH
                     PEIPPLSREDAGQGTGAVNGPAHAQSKYSVHYDEGVKVGYKWYEAEHKEPLFPFGYGL
                     SYTTYSYSGLKTDSAQKIVTFSVKNTGQREGTEIAEVYAMLPEAAGEPFKRLVGWQRV
                     DLKPGESKTVTVTVDPLMLSIFDEQKDGWSLLPGTYKILAGPSSAETPLHGAFEIH"
     gene            complement(3847..4584)
                     /locus_tag="VFA02_07860"
     CDS             complement(3847..4584)
                     /locus_tag="VFA02_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09805.1"
                     /translation="MAEIAQIELLPAQKVAQETHILQAPNGVCYAQFGDTHLPAQELE
                     RIVQAVPSGVAAALKRRAYYFVPLTISEEDTTLVAPAYTIDFSDRAICHRNVRFGNSE
                     CIFISTRLMQDRFALAFEFFINVGRHFVDEAGVPESFSALAWSQVQADVRGETSHDAW
                     EHRRRATTPGQVDEKAKNQFFEAAFADAISIYMLSLTVDFDYMDLREREYPLLAAPAL
                     AERLRHVVELFPSNPGYEFEIRYRRRS"
     gene            4606..4878
                     /locus_tag="VFA02_07865"
     CDS             4606..4878
                     /locus_tag="VFA02_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09806.1"
                     /translation="MGSATHLHSGRISEFGFVASLLMAVALGIAAFFATCFLAIFGIL
                     FYNVAGHHVDFANSYKFFALPAGAGVLAISLVVFLGLWLRRKFRTA"
     gene            4926..5840
                     /locus_tag="VFA02_07870"
     CDS             4926..5840
                     /locus_tag="VFA02_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HZU09807.1"
                     /translation="MPVITISTETLASRTPKQIRQWCVQLEAGDILYFPQTPIQLSQN
                     DIEFLLGQQQTDSLLHKNIAYKPNKDKLSGVDTETADAQAVVRLQTIMRQYSQSVAEF
                     LTQFLAPYKANWQLDYASFRPQEEEGRDLPLRRRNDLLHTDAFPTRPTYGARILRFFN
                     NIHPSRTRDWVVSDPFAEMVKHFAPQQIAPHADGPFARVAKSLGRGIGLGAVIPSIKR
                     SPYDDFMMRFHNFLKENPRFQSECPKYKFNFPPGSSWMVYTDTVPHAVLAGRYALEQT
                     FLVRPEVLVAPESSPLKVLEAIAGAKLV"
     gene            6079..6849
                     /locus_tag="VFA02_07875"
     CDS             6079..6849
                     /locus_tag="VFA02_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09808.1"
                     /translation="MNFTAALPDGFVVVPPNPIMQYSAMMAQMRGIPQPLSMFLLLPP
                     GSQLGQFTPAIVVVEELDQTTAQVAMMGMQNLESPDVAWGLAQSLQVQYVFNFTPLRQ
                     IPLQTGTALGREFDAIGMPPFNVTIHVLVLMIQGLQSTVKVMVMVQTQRWSEFLAPCL
                     QFVGGINNSGGNPLPSSVIAVADPRHPDQVQLNIVNPKTRQQMPVMSVPTGAVYNVTI
                     HINDHSVNVNGSIQGAGIAVGEHSLASTAVRAETGANL"
     gene            6846..7259
                     /locus_tag="VFA02_07880"
     CDS             6846..7259
                     /locus_tag="VFA02_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09809.1"
                     /translation="MTDNSGTSGTYVGGGISGTGIAVGAGASANATIGNAADQQQLIK
                     LIEELKAEIQKSSIPDAMKSTIVESVVQPMSKDVKAPEAKSKLESGLEKLNLLVKASG
                     AAQSELATIGNIAGTIAKFCGIAFHTVAPFLAGVL"
     gene            complement(7254..8246)
                     /locus_tag="VFA02_07885"
     CDS             complement(7254..8246)
                     /locus_tag="VFA02_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HZU09810.1"
                     /translation="MRKRIGHALIYLVLFLLTVWAVLALSFDVRIAWLRWPAIGLYLA
                     TVIWAIFRCCRFWRRVLSVVACFLIVLGWWLSLRPSNGGNWQPDVSRLAYAQINGKHI
                     TIYNTRDCDYRAEFDYTCQWPTREIDLAQIRGIDLFLDYWGSPWIAHTILSFDLGNGQ
                     HIAFSIEARKHVGQHYSAIRGFFRQYTLISVVGDERDVVRLRTNYRQGEDLYLYHTTA
                     TPVFAQSLFLDYLHFTNYLHDHPKWYNAVTHNCTTEIFALQTMKSQPRNWTILLNGKG
                     DEAQYREGKIAGDGLPFAALKQRAYINPAAKAADNDPNFSQKIRQGRPGFGSNL"
     gene            complement(8280..9401)
                     /locus_tag="VFA02_07890"
     CDS             complement(8280..9401)
                     /locus_tag="VFA02_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZU09811.1"
                     /translation="MNTTAFLPRIWVLPGILAACLCVAGSAQTPFLEKVPTSPSLTPL
                     TMIIGGRVLHSGANEYVYQWPGTYFETAFRGPELYLRVRTNHEILHVVVDGHPALVLS
                     KPQPGVYRLSGLKNRRHDVRVLVVTESQDAPNVFGGFGISAGERALQLRTPHRQIEFI
                     GDSHTVGYGNTSPKRDCTNDEVWATTDNSQNFGPLTANHYHADYQINAISGRGIVRNY
                     NGFAADTLPVAYPYVLFDKKQQYSDVEWKPQIIVIALGTNDFSTPLNPGEKWKTRDEL
                     HSDYETTYLHFLQFLRARDPGAYIIVWATDMANGEIESEAQKVVQQMKAQGETKIAFL
                     PMDHLQFSGCHWHPSVADDKTIRDKLVQFIDAHKLWKEE"
BASE COUNT         2282 a         2390 c         2642 g         2163 t
ORIGIN      
        1 ctggcaatca aaaggtgcac gcacgctcgt tacgattcct gtattgatgg cctgcgaaag
       61 gtccccgcca taggtgctgg taaaagggac accaacattg aagttgttgc cctggatcag
      121 gctgcgccat tccatgttgt agaaaaagaa tgtcttcggg ttcccaccgc catgcagcgc
      181 cacaggaccg cccacattga atccccacag attaaaccgc agcttagcga ctttattatg
      241 tgtaccatcg gcgctgtact gtggattaaa gtaattgcgt gcgttaaagg cgtcattgcg
      301 tccgaaccac catcctgaag catgaaagtt tctggtgccc gatttaatca cggtgctgat
      361 ggttgcagca gaagatagac catactgggc gctgtagttt gacgtgagct ggcgaaactc
      421 ggcaagcgca tcttctgatg gcataacgat ggcgctgcca ccgccgccac ggtcgtccgt
      481 ctcgccgccg tccagcagaa agaggttgtg gctgttgcgc tgtccgttga agctgacgct
      541 ggagtcgccg cccatgggcg taggaacctg gaagtccatc tgaaggctcg acgcccccgt
      601 ggtcagattc atgagcgaat agaggctacg gccattcgat tccaattgcg taatctgttt
      661 gccggagatg acattgctca cttctccgga gtcggtctgt agcttgatgc ctgatgcgga
      721 aacagtaacc gtttcctgtg ccgcaccaac ttgcagggta aagtttacgc gcgcacggtc
      781 tcctacgttg agcacgacac cagtcttcgt ctgcacgttg aagccagcgg cctccacttt
      841 gatggcatag gtgccaatct gtaaagcggg cagaacgtac tcgcccgttc cattggaagt
      901 ggcactgtgc gtaatgcctg ttgcggtatc cgttacggta atcgtggcgt ttggtacagc
      961 cgatccggtt gggtcagtaa tggtcccaac gatggttgcg gtcgctgagg tttgagcaga
     1021 cgcagtcagc gagaacaaag taaataatat gactgtcatc cgcatgaaaa tcctgcgaca
     1081 tgcggcctcc cgaaaaacca gggatttcat gtttcctcct tttaaaagat taatttttcg
     1141 atttactaaa gcgatttatt ttatccgcag agaatttaac cctcttgaaa gaagatttgt
     1201 caagaagaaa tcatgtatag gcttcagcaa gcctgaatga ccaaatgttt tcaatgagtt
     1261 aataggaaaa ccaggaggtg gtacccggtg ttgatcctgt ctgccaggac gcttttttga
     1321 gatcaatgca agcctgttcg gacgaaagga cggtatgtcc ctctgcaaat accctgaaat
     1381 aaaaattcac ttgatgatca tgaccagttt tatacttatt aaacaaacgt ttgctttaaa
     1441 aataagacct gcactgcttg gatatcttat ttaaataaaa cgtttgagct tgacgaacat
     1501 ctttccagga ctttagcatc tccacgaaaa tacctctgag aggaagaact ttttgatgtt
     1561 gattcacact ttgaggaaat gtctgtcact tgcgctgctt gcggcctgtt ccattgccct
     1621 tgcccagaac aatccacagg accgaccctg gatgaatacc agcctctcgc ctgaaaagcg
     1681 tgccgacctt gtgctgaagc agatgacgct cgacgagaag attgacctgc tgcatggcaa
     1741 cggaatggcg cataccggga actggcaaat gccgctgacg cacctgtcaa atggcggcgc
     1801 gggttacgta gtaggtgtgc cgcgactggg aattcctccg ctctacattt cagatgcggc
     1861 ctatggcgta cgttccagtg gcgagaatgg ccgctattcc actgctctgc cctcgaacct
     1921 tggcgcggca tcaagctgga acccgcaggc tgcgtgcgag tatggtgcct tgatcggccg
     1981 cgaactgcgc gcgcaaggct acaatatgac gctcggcggg ggcgtgaatc tgacccgaga
     2041 gccgcgtaac ggacgcacct ttgagtacat gggcgaagac ccgctgctgg ctggaaccat
     2101 ggtcggcaac ctgatgaagt gcgaacaagg cgagcatgtg gtcggcgaca tcaagcatta
     2161 cgcaatgaac gaccaggaga ccggccgcaa tatcgtcaat gtcatcgtct caaaacgcgc
     2221 catgcgcgaa agcgatcttc ttgcatttca gattggactg aaaatttcca atgccgccgc
     2281 ggcaatgtgc tcctacaacc gcgtgaatgg tgattattcc tgcgaaaaca agtatctgct
     2341 gacggacgtt ctgaagcacg actgggactt caaaggcttc gtactttccg actggggtgg
     2401 cacgcacagc acggagaagg cctcggctgc gggtctggac caggagcagc ccatggccga
     2461 ttactttggg ccgaagctga aagaagccgt cgaagcagga cgcgtaccca tgtctgaagt
     2521 tgatgaccat gcgcgccgag tgctctgggc cgaattcgct tcaggcattg tggatgatcc
     2581 ggtgcagaag agcgtcgtgg atgccgaagg aggcctggaa atcgcgcagc gcctggaaga
     2641 acagagcatt gttttgctga agaatgaaca tgatattctg ccactcaatc cggcaaagct
     2701 gcacagcttg gctgtgattg gcggacacgc cgatgtgggg atgatctcag gtggaggttc
     2761 ggcacaggtg gacccacccg gagggaatgc cattgctcct ccgggacaca aagcaacaac
     2821 gtggcaggcg catatctggt ttccaacgtc tccgctgaaa gcactgcgcg caaagctgcc
     2881 gaatgcgaag gttgaataca actctggtga agacctggat gcagcagcgg cgttggcgaa
     2941 ggactctgat gtggcgattg tgtttgccta tcagtgggaa tcagaaggaa tggacctgcc
     3001 gaacctttcc cttcccgaca atcaggatgc cttaatcgaa cgtgtggcag cagccaatcc
     3061 ccacacgatt gtggtactcg aaaccgggac cgctgtcacg atgccgtgga tcaacaaagt
     3121 cagcggcatc gtggaagcat ggtacgcagg cagcagcgga catcgtgccc ttgcgaacgt
     3181 cttgttagga gaagtgaatc ccagcgcaaa gctcgccatg accttcccta agagcgagca
     3241 ggaccttccg catcctgaga ttccgccatt gtcgcgggaa gatgccgggc aggggaccgg
     3301 cgccgtgaac ggtccagcac acgcgcaatc gaaatatagc gtgcattatg acgaaggagt
     3361 aaaggtcggt tataagtggt acgaagcaga gcacaaagaa ccgctctttc cctttggata
     3421 cgggctgtcg tacacaacgt attcttattc tgggttgaag acagacagcg cacagaagat
     3481 tgtcactttc agcgtaaaga atactggcca acgcgaaggc acggaaatcg cagaagtata
     3541 cgcaatgcta cccgaggccg ccggggaacc attcaaacgg ctcgtaggct ggcagcgcgt
     3601 tgacctgaag ccgggtgaat cgaaaacagt cactgttacg gtagatccgc tcatgctctc
     3661 gatttttgac gagcagaaag atggatggag cctgctgcct gggacttaca aaattcttgc
     3721 cggcccgtca tcggcagaaa ccccgctgca cggagcattt gagattcact gattcagaaa
     3781 gaaaaaagcc atagcgggga agcattcttc cgctatggct ttttatctat aagcagcatg
     3841 aatgccttag ctccgtcgcc ggtagcggat ctcaaattca taaccagggt tcgagggaaa
     3901 caactcgacc acgtgccgca ggcgttccgc caaagcagga gcggcaagga gcggatactc
     3961 gcgttcccgc agatccatat agtcgaaatc cacagtcaac gagagcatgt agatcgagat
     4021 ggcatcggca aaagctgcct cgaagaactg gttctttgcc ttctcatcta cctgaccggg
     4081 cgtagtagcg cggcggcggt gctcccaggc atcatggctg gtttcacccc ggacatcagc
     4141 ctgcacctga gaccaggcaa gggctgaaaa gctctccggc actccggctt cgtccacaaa
     4201 gtggcgtcca acgttgatga agaactcaaa ggccagtgca aaacggtcct gcatgaggcg
     4261 ggtagaaata aaaatgcact cggaattgcc gaagcgcacg ttgcgatgac agatggcacg
     4321 gtcgctgaag tcaatggtat atgccggggc gaccagggtc gtatcctctt cactaatggt
     4381 aagaggaaca aagtaataag cacgacgttt gagggcggca gcaacaccgg aaggaacagc
     4441 ctgcacgatg cgttccagtt cctgcgcggg caggtgggta tcgccaaact gcgcgtaaca
     4501 gacgccattc ggggcctgaa gaatatgcgt ttcctgcgcc actttctggg cgggcagcag
     4561 ctctatttgg gcgatttctg ccataaactt gtattctagc tggttatggg atctgctaca
     4621 catttacaca gcggcaggat ttccgagttc ggctttgttg ccagtctgtt gatggctgtt
     4681 gcactgggaa tagcagcctt ttttgcaacc tgctttctgg ccatctttgg cattcttttt
     4741 tataacgtag cggggcatca tgtggacttt gccaacagct ataaattttt tgcgcttccg
     4801 gcaggagcgg gcgtgcttgc cataagcctg gttgtgttcc tcggtttgtg gctgcgacgg
     4861 aagtttcgca ctgcgtgaac gctcaagtcc gggccggaat ggcagaggag taactcagag
     4921 tttttatgcc tgtaataacg atttcgacag agacgctggc atcgcgcacg ccgaaacaga
     4981 tacgccaatg gtgcgtccag cttgaggctg gagacattct gtattttccg cagacgccga
     5041 ttcagctttc gcagaatgat attgaatttc tgctggggca acaacaaacc gacagtttgc
     5101 tgcacaagaa catcgcctat aagccaaaca aagataaact gagcggcgtg gacaccgaga
     5161 ccgccgacgc acaggcggtg gtgcggttgc agaccatcat gcggcagtat tcgcagagcg
     5221 tggcggaatt cctgacgcag ttccttgcgc cgtataaggc caactggcaa cttgattacg
     5281 ccagcttccg tccgcaggaa gaagagggcc gcgatcttcc gttacggcgc cgcaacgatc
     5341 ttctgcacac agatgcattt cctactcgac cgacatatgg cgcacgcatc ctgcgcttct
     5401 ttaacaacat tcatccatcg cggacacgcg actgggtggt gagtgatccg tttgcggaga
     5461 tggtgaaaca ttttgcacca cagcagattg ctccgcatgc agatggcccc tttgcacgtg
     5521 ttgcaaaatc actgggccgc ggcattgggc ttggggcagt aattccgtct atcaagcgct
     5581 ccccatatga cgacttcatg atgcgcttcc acaattttct gaaagagaac ccgcgctttc
     5641 agtcagagtg tccgaaatac aagttcaact tcccgccggg atcaagctgg atggtctata
     5701 cggacaccgt tccgcacgct gtgctggcag gtcgatatgc gttggaacag acattcctgg
     5761 tgcggcctga ggtgctggtt gcaccggaga gttctccgct gaaggtgctg gaagcaattg
     5821 ccggagcaaa gctggtgtaa caacaaaaaa acctgctttt cgtctcggct aatgatattt
     5881 cgttgcgggt ctgtcggatt tccttgcaat cgcagtaaga gcaggccctt cccctacgcg
     5941 ctgcgcgctc cgttcagaat agtgggctga tcttctctgt gtggatgaga agacccggac
     6001 agctaaaaat aatttctgtg tgcatgcgcg ggcctgagct atacttctcg cactttgggt
     6061 ggagaagtca tccgcggagt gaattttacg gcagcgctgc cggatgggtt tgttgttgtt
     6121 cctccgaacc ccataatgca gtactccgct atgatggcgc agatgcgcgg cattccgcag
     6181 ccgttgagca tgtttctgct gcttccccct ggttcgcagc tgggccagtt tacgcctgcc
     6241 attgtggtag tggaagagct ggatcaaaca actgctcagg tagccatgat gggaatgcaa
     6301 aaccttgagt cgccagatgt ggcgtgggga ctggcgcagt ctttacaggt gcaatatgtg
     6361 ttcaacttca cgccactgcg gcagattcct ctgcagaccg gcacggcttt aggcagggaa
     6421 tttgacgcga ttggcatgcc tccatttaac gtcacgatcc atgtgcttgt attgatgatc
     6481 caggggttgc aatccaccgt gaaggtgatg gtgatggtgc agacacagcg atggagtgag
     6541 tttctggccc catgtttgca gtttgttgga ggcatcaaca actctggcgg aaacccattg
     6601 ccgtcttctg tgattgctgt ggcagatccg aggcatccgg accaggtgca attgaacatc
     6661 gtcaatccaa agaccaggca gcagatgccg gtgatgtccg ttcccaccgg cgcagtctac
     6721 aacgtcacca ttcatatcaa tgatcactcg gttaatgtga atgggagcat tcagggcgct
     6781 ggaatcgcag taggcgagca cagtcttgca tctaccgcag tccgcgcaga aacgggagcc
     6841 aatttatgac agacaacagc ggaaccagcg ggacctatgt gggcggcgga atctctggca
     6901 caggcatagc cgtcggcgca ggcgcttctg ccaatgccac gattggaaat gctgccgacc
     6961 agcagcagtt aatcaaactg attgaagaac tgaaagctga gattcagaag tccagcatac
     7021 ccgatgcgat gaaaagcacg attgttgaaa gcgttgtaca acctatgagt aaggacgtga
     7081 aggccccgga agcgaagagc aagctggagt cagggcttga gaagctgaat ctgctggtca
     7141 aggcatcagg cgcagcacag agtgagttgg ccacaatcgg aaacattgcc ggaacgattg
     7201 cgaaattttg cggcattgca tttcatactg tggcaccgtt ccttgctggc gtgttataag
     7261 ttactgccga acccgggccg gccctgacga attttctgtg aaaaattcgg gtcattgtcc
     7321 gccgcttttg ccgcaggatt gatataggcc cgctgcttta atgcagcaaa tggcaggcca
     7381 tctccggcaa tcttaccctc gcggtattgt gcctcatctc ctttgccgtt gagaagaatg
     7441 gtccagttcc ggggctgact cttcatggtc tgtaaagcaa agatttccgt tgtgcaattg
     7501 tgtgttacag cgttgtacca cttagggtgg tcatgcagat aatttgtaaa gtgaagatag
     7561 tcgagaaaga gcgactgcgc gaagaccgga gttgcagtgg tgtggtacag atacagatct
     7621 tcaccctgac ggtaattggt gcgcaggcgt acgacatcgc gctcatcgcc cacgacagag
     7681 atcagagtgt actggcggaa gaatccgcga atggcagaat aatgttgccc gacgtgcttg
     7741 cgcgcctcaa ttgaaaaggc gatgtgctgg ccgtttccca ggtcgaagct caggatagtg
     7801 tgggctatcc agggtgatcc ccagtaatcg agaaacaaat caatgccgcg tatttgggcc
     7861 aggtctattt cccgtgtcgg ccactggcag gtgtaatcga attcggcgcg atagtcacaa
     7921 tcgcgtgtgt tgtagatggt gatgtgctta ccattgatct gagcataagc aaggcgtgag
     7981 acgtccggct gccaattgcc gccgttggag ggccggagcg aaagccacca tccgagaacg
     8041 atcagaaaac atgcaaccac tgacaagaca cggcgccaga aacggcagca ccggaagatg
     8101 gcccagatga cggtagcgag atagaggccg attgcaggcc agcgaagcca tgcaatgcgc
     8161 acgtcgaaag aaagcgccag cacagcccac acagtaagca ggaacaatac aagataaatg
     8221 agggcatgcc caatccgttt tctcataaaa taacgcttta ctccgggacg aattctgtac
     8281 tactcctctt tccagagctt gtgcgcgtca ataaattgca caagtttgtc tcggatcgtc
     8341 ttgtcgtctg caacggaagg gtgccagtgg catccgctga actggaggtg gtccattgga
     8401 aggaatgcga tttttgtctc gccttgcgcc ttcatctgct gtaccacttt ttgggcttct
     8461 gactcgatct cgccattggc catgtcggtg gcccagacaa tgatgtaggc accagggtct
     8521 cgcgcgcgaa ggaactgaag aaagtgcaga tatgttgtct cgtaatcgga gtggagctca
     8581 tctctggtct tccacttttc gccgggattc agtggcgtag aaaaatcgtt agtgcctaac
     8641 gcaatcacaa tgatctgcgg cttccattca acgtcgctgt attgctgttt tttatcgaag
     8701 agcacatagg ggtatgcgac gggtagtgta tctgcggcaa agccgttata gttgcggacg
     8761 attccgcgac cggagatggc gttgatctga tagtcagcgt ggtagtgatt ggcagtaagc
     8821 ggaccgaagt tctgtgaatt gtcggttgtg gcccagactt catcattggt gcagtcccgt
     8881 tttggagagg tgtttccata gccaactgta tgcgaatcgc caatgaactc aatctgacga
     8941 tgtggtgttc gcagttgcag cgcacgttca cctgcagaaa ttccgaagcc gccgaagacg
     9001 tttggcgcgt cctggctctc tgtaacaacc agtactctga cgtcgtggcg cctgtttttc
     9061 agccctgaaa gcctgtacac gccaggttgt ggcttactca ataccagagc gggatgccca
     9121 tccaccacga cgtgaagaat ttcgtggttc gttctcacac gaagatagag ttcaggcccc
     9181 cgaaatgctg tctcaaaata agtccccggc cactggtata catactcgtt tgcgccagaa
     9241 tgcagcactc ttccgccgat gatcatagtc agcggggtca gcgaaggcga agtcgggact
     9301 ttttcaagga atggagtctg tgccgaacca gccacgcaga ggcaggctgc aagaatacct
     9361 ggaaggaccc aaatccgggg cagaaaagca gttgtgttca tggggaaatt caggataacg
     9421 cattttgata aaagctgatt tttatcaaaa tttcaaagga aggcgaaggc aaaaacc
//
LOCUS       DASRBV010000208         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_676827, whole genome shotgun sequence.
ACCESSION   DASRBV010000208 DASRBV010000000
VERSION     DASRBV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676827"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="VFA02_07895"
     CDS             <1..2172
                     /locus_tag="VFA02_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZU09812.1"
                     /translation="KMKPDGTLDWTPPPGRWAILRIGYSLLGITNHPATAEATGLEVD
                     KLNYVYVKNYMDHYLDSYKDTVGADMMGKRGIEYVITDSWEAGSQNWTDDMLEQFKKR
                     RGYDPRPWLPVLAGHIVESSEASDRFLWDFRKTIADLIADEHYGQVQASLKERGMGHY
                     GESHESGRAFVADGMEVKKLNDIPMSAMWTQVPGVYKEQYGYDADDRESASVAHIYGQ
                     NIAAAESLTAAAAAWAWSPATLKPVADQELLNGINRFVIHESAHQPLVGKAPGLTLGP
                     FGQWFNRNETWAEEAGPWVDYLARSSYMLQQGHFGADLVYFYGEDSNLTAIFANKWPD
                     IPAGYGFDYINADGLIHELGVSDGRITTKSGMNYRVLGLDPYSRHMSLPVLRAIHRLV
                     EDGAVVAGPKPVDDPSLADDQAEFHRLADELFGDGTGVHHVGKGIVYAGARMADAFHA
                     LNLAPDFDSTGAEGENAIRFAHRRLSDGDIYFVDNQTDRPQKIEATFRVTGYAPELWY
                     AETGKSQPVSYKIADGRTTVPLQLEPWGTVFVVFHKKTVANSRTLADPVETQLTTVEG
                     PWTVSFQPDRGAPPSITLNTLSSWSDNTDPGVKYFSGTGTYTKTIQAPASWFKKGASI
                     WIDLGDVKNLADVSVNGKDLGIVWHGPFRVDATHALKPGANQIQVKVTNAWVNRLIGD
                     QQPNATTKYTFTDIKPYKANSPLLPSGLLGPVRVYSVLEAR"
     gene            complement(2187..2654)
                     /locus_tag="VFA02_07900"
     CDS             complement(2187..2654)
                     /locus_tag="VFA02_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU09813.1"
                     /translation="MSKPSQPANMSGALSDPTLSPGVAFLFQLEAKFAQDTAKGGGKA
                     FASWFSDDGVALANGKAPVRGRAAIAAQATWDPKDYQLTWTPQGGQMSPSGDMGFTWG
                     HYEGRAKDHSGNTVSTSGRYMTIWKKQPDGSWKVALDSSSDEPPAAGDCCKLP"
     gene            2861..>4210
                     /locus_tag="VFA02_07905"
     CDS             2861..>4210
                     /locus_tag="VFA02_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZU09814.1"
                     /translation="MSQQDTSLPSRAEFLKLAQTHTLVPLYRTLTADLETPVTAFLRL
                     AADEPECFLLESVEQGEKIGRYTFIGIRPFRRIVSRGREIEITENGKTRRLEGDIFAL
                     TKELLTSHRPARIAGLPPFTAGAVGFFAYDVVRQIERLPEKAKDDLGLPDACLMFFHE
                     VLAFDHVRKEMLLIATADVSQQKPEQAYTDALRRLGRLEKRLAIPLPRLRQKKNGGKL
                     KLEPRTRKRNFLKAVEKAKEYIAAGDIFQVVLSQRFDVETGVDPFSVYRALRIVNPSP
                     YLYFLRTGLKKPGDTQIAGSSPELLVRVTQGKIQYRPIAGTKPRGETEEDDRRIADEM
                     LHDEKERAEHVMLVDLGRNDVGRVSEYGSVRVKELMVVERYSHVMHIVSGIEGRLRPE
                     LHAVDAFRACFPAGTLSGAPKVRAMEIIEELEPTRRGIYGGSVFYADYSGNLDSCIAI
                     "
BASE COUNT          933 a         1084 c         1293 g          900 t
ORIGIN      
        1 aagatgaagc cggatggcac gctcgactgg acgcctccgc ccggcaggtg ggccattctg
       61 cgcattggat attcgctgct tggcatcacc aatcatccgg ccacggcgga ggcaaccgga
      121 ctggaagtgg acaagctgaa ctatgtttat gtgaagaact acatggacca ctatctggat
      181 agctacaagg acacggtcgg tgcggacatg atgggcaagc gcggcatcga atacgtgatt
      241 accgatagct gggaggcagg atcacagaac tggactgatg acatgcttga acagttcaaa
      301 aagcggcgcg gatacgatcc gagaccgtgg ttgcctgtgc tcgctgggca cattgtcgaa
      361 agctctgaag cgagtgatcg ctttttgtgg gacttccgca agaccatcgc tgacctgatt
      421 gccgatgagc actacgggca ggtacaggct tcccttaaag agcgcggcat gggccattac
      481 ggagagtcgc acgagagcgg acgcgctttt gttgccgatg gcatggaagt gaagaagctc
      541 aatgacattc cgatgagtgc catgtggacg caggtaccgg gtgtctacaa agagcagtat
      601 ggctatgatg cagacgaccg cgaatcagcg tctgtagcgc acatctatgg gcagaacatc
      661 gctgctgcgg agtcgctgac ggccgcggct gcggcgtggg cgtggtctcc ggcgacgctg
      721 aagccagtgg ctgatcagga gcttctgaat ggaatcaacc gtttcgtgat tcatgagtcg
      781 gcgcaccagc ctctggtggg gaaagctccc gggctgacac tggggccttt cgggcagtgg
      841 ttcaaccgca atgagacgtg ggcggaagaa gccggcccgt gggtggatta ccttgcacgc
      901 agcagctata tgctccagca ggggcacttc ggcgcggacc tggtgtactt ctacggtgag
      961 gattcaaacc tgacggcgat ctttgccaac aaatggccag acattcccgc aggctatgga
     1021 ttcgactaca tcaacgccga tggattgatt cacgagctgg gcgtttctga tggccgcatt
     1081 acgacaaaga gcggtatgaa ttatcgcgtg ctgggactgg acccttacag ccggcatatg
     1141 tctttgccgg tgctgcgggc gattcatagg ctggtagaag acggcgctgt tgttgctgga
     1201 ccgaagccgg tggatgatcc aagtctggct gatgatcagg cggagtttca caggctggcg
     1261 gatgaactct tcggcgatgg tactggagta catcatgtgg gcaaaggcat tgtctatgcc
     1321 ggtgcgcgca tggccgatgc ttttcatgcg ctcaatctcg cgcctgattt cgactcgacg
     1381 ggtgccgaag gtgaaaatgc catccgcttt gcgcatcgca ggctgagtga tggcgatatc
     1441 tattttgtgg acaatcagac cgaccggccg cagaagattg aggccacctt ccgtgtgaca
     1501 ggatatgcgc ctgaactctg gtatgcagag acaggcaaat ctcagccggt ctcttacaaa
     1561 attgcggacg gacgcacgac ggtcccgttg caactggaac cgtggggtac ggtgtttgtg
     1621 gtcttccata aaaagacggt cgcgaattcg cgcacgctgg ctgatccggt cgagacacaa
     1681 ctgacaaccg tggaaggtcc gtggacggtc agcttccagc cggaccgtgg tgctccacct
     1741 tctatcacgc tgaatacact gtcgtcgtgg agtgacaata ctgatccggg tgtgaagtat
     1801 ttctccggca ccggaactta cacgaagact attcaggcgc cggcaagttg gttcaaaaag
     1861 ggcgcatcca tctggataga cctgggggat gtgaagaacc tggctgatgt ttcggtgaat
     1921 ggtaaggatc tgggcatcgt ctggcacggg ccgttccgcg tagacgcgac gcatgcgctg
     1981 aagccgggag caaaccagat tcaggtaaaa gtgacaaacg catgggtgaa tcgcctgatt
     2041 ggcgaccagc agcccaatgc gacaaccaaa tatacgttca ccgatataaa gccgtataag
     2101 gcgaattcac cgctgctgcc ctcagggttg ctggggccgg tgcgggtgta ttcggttttg
     2161 gaagcgcggt agtcgagaac gaaccatcac ggaagcttac agcaatctcc agcagcaggc
     2221 ggttcgtcgc tgctggagtc cagcgcaact ttccaggaac cgtcaggctg ctttttccag
     2281 atggtcatgt agcggcctga tgtggagacg gtgttgccgc tgtggtcttt ggcgcggcct
     2341 tcgtaatgtc cccaggtaaa gcccatgtcg ccggaagggg acatctggcc gccctgtggt
     2401 gtccaggtga gctgataatc tttcgggtcc catgtggcct gggcggcgat ggcggcgcgt
     2461 ccgcgcacgg gggccttgcc gttggcgagg gcgacgccat catcggaaaa ccaggaggcg
     2521 aaggccttgc cgccgccttt ggcggtgtcc tgcgcaaact ttgcctcaag ctggaagaga
     2581 aaggcgacgc caggtgaaag cgtcgggtca ctgagcgcgc ctgacatatt ggcgggctgt
     2641 gagggtttgg acaaaggatc tagctgaagt tgctgcgcaa atgccggtgc gcaaaccatc
     2701 aggcagagaa cggaccatcg cattgcaaaa attacaacct ccaactgatt atgctacgcc
     2761 gttttcctga cctgctcccc cctaaactgc ggtttatgat caacatcttt tactgtcctg
     2821 tccgtcctgc ccgtttgtct tcctcatata ctggaagcac atgtcacagc aagatacatc
     2881 gcttcccagc cgcgccgaat ttctgaagct ggcacaaacg catacgcttg tgccgctcta
     2941 tcgcacgctg actgccgatc ttgagacgcc ggtcacggct tttctgcgcc ttgctgccga
     3001 tgaaccagag tgttttctgc ttgaatctgt cgagcagggt gaaaagattg gccgctatac
     3061 ctttatcggc attcgtccat ttcgcagaat agtgtcgcgt ggacgggaaa tcgagatcac
     3121 cgagaacggg aagacacgcc gccttgaggg cgacattttt gcgctgacga aggaactgct
     3181 caccagccat agaccagcgc gcatcgcagg gctgccgccg tttactgctg gtgcagtcgg
     3241 gttctttgct tacgatgtag tgcgccagat tgagcgcctt cctgaaaaag caaaggacga
     3301 tctcggactg ccggatgcct gcctgatgtt ctttcatgag gtgctggcct ttgatcatgt
     3361 ccgcaaggag atgctgctga ttgccacggc ggatgtttct cagcagaagc ctgagcaggc
     3421 gtacacggat gcgctcaggc gcctgggccg gttggagaag cgcctggcca taccattgcc
     3481 gcgcctgcga cagaaaaaaa acggcggcaa gctgaaactg gaaccgcgca cgcgaaagcg
     3541 aaatttcctg aaggccgtgg agaaggccaa agaatacatc gctgcaggag acatctttca
     3601 ggtggtgctt tcacagcgct ttgatgtgga aacaggcgta gatcccttct cggtctatcg
     3661 cgcgctgcgc atcgtgaatc catcgccata tctgtatttt ctgcgcacag ggctgaagaa
     3721 gccgggcgat acgcagattg cgggctcctc accagagctt ctggtgaggg tcactcaggg
     3781 aaagatacag taccgcccga ttgcaggtac aaagccgcgt ggtgaaacgg aagaagacga
     3841 ccgacggatt gcagatgaaa tgctgcacga cgaaaaagaa cgcgccgagc atgtaatgct
     3901 tgtagacctt ggccgcaatg acgtgggccg tgtcagtgaa tatggttcag tccgagtaaa
     3961 agagctgatg gtggtggagc gctattcgca tgtcatgcac attgtcagcg gaattgaagg
     4021 caggctgcgt ccggaactcc atgccgtgga tgcgtttcgc gcctgtttcc ccgctggaac
     4081 attgagcgga gcgccaaaag tgcgcgcgat ggagatcatc gaagaactgg aaccgacgcg
     4141 gcgcggcatt tatggcggaa gcgtatttta tgctgactat tcaggaaatc tcgattcgtg
     4201 cattgcgatc
//
LOCUS       DASRBV010000209         9015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_316673, whole genome shotgun sequence.
ACCESSION   DASRBV010000209 DASRBV010000000
VERSION     DASRBV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316673"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFA02_07910"
     CDS             complement(<1..270)
                     /locus_tag="VFA02_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09815.1"
                     /translation="MHVAHGFHLAALTEGRVWWMPPDGSLHGPAADHLMWFNLAALGA
                     LFLLAQVLLVLVFLRRAKRKTCSSAPLSTQDDKWGMWRYELLPLIA"
     gene            complement(285..896)
                     /gene="pdxT"
                     /locus_tag="VFA02_07915"
     CDS             complement(285..896)
                     /gene="pdxT"
                     /locus_tag="VFA02_07915"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZU09816.1"
                     /translation="MTPGSGNASQLRIGVLAIQGDYAAHAQALREAGAEAVLVRKPEE
                     LANIDGLVIPGGESTTFLKFLARDGFLDALQAFVRQKPTFGTCAGCILLAKEVRNPSQ
                     ESLAVLDATVERNAYGRQIDSSIQIAETKIPGGPLEMVYIRAPRIVKTGPGVEVLADR
                     DGFPVLVREKNILAATFHPELSSDRRVHRIFLDLVREQQKQQV"
     gene            complement(893..1381)
                     /locus_tag="VFA02_07920"
     CDS             complement(893..1381)
                     /locus_tag="VFA02_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09817.1"
                     /translation="MIVVADTSPINYLIEIGHVNVLPQLYSRVLVPQRVVEELREVQA
                     PEKVRTWMDNLPEWFEVVTCPSHINLSIAHLDPGEQEAITLSREMHADYLLVDDMAAR
                     TAATQLHVQVIGTLGILTRASYHNLLDLPTALAALQETSFFAAPELIQQLLLEDARRK
                     KG"
     gene            complement(1378..1653)
                     /locus_tag="VFA02_07925"
     CDS             complement(1378..1653)
                     /locus_tag="VFA02_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HZU09818.1"
                     /translation="MNMHFDLQVPDDVGTVLQAQYSDLSRAALEALALEGYRAGSLTE
                     SQVRRLLGFSTRMQVHAFLKEHGVHLNYSMKDLEQDITAAYEQKRSA"
     gene            complement(1675..2628)
                     /gene="pdxS"
                     /locus_tag="VFA02_07930"
     CDS             complement(1675..2628)
                     /gene="pdxS"
                     /locus_tag="VFA02_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZU09819.1"
                     /translation="MTEQYANNGTSTTSLRLKVGLAEMLKGGVIMDVMNVEQARIAEE
                     AGAVSVMALERVPAMIRAEGGVARMANPKLIKEIMAAVSIPVMAKARIGHFAEAQVLQ
                     EIGVDFIDESEVLTPADEANHIDKHAFTTPFVCGARDLGEALRRIAEGAAMIRTKGEA
                     GTGDVVHAVKHMRQITSDIRSLTVLRDDELYVAAKELRAPYELVRMVAKNGKLPVPNF
                     SAGGIATPADAALMMQLGAESVFVGSGIFMKERATPLDVENNPEERAEAVSRARAIVI
                     ATTHYNDPKIVAEASEQVTGTMKGLAVAAIEQEQLLQTRGW"
     gene            complement(2720..3166)
                     /locus_tag="VFA02_07935"
     CDS             complement(2720..3166)
                     /locus_tag="VFA02_07935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZU09820.1"
                     /translation="MNTKTLTPLAHGALNRCFGCGPENRSGLRLRFYVDADQQIVCHV
                     RLAQRFEGPPGHAHGGIIATLLDEAMSKANRVHGTTAMTHQIEVEYLRPVPLKQPLTL
                     TARRSSHQGRRNFCEAEIQDASGTILARSKALFIVVDHNKLAQLRA"
     gene            complement(3163..4125)
                     /locus_tag="VFA02_07940"
     CDS             complement(3163..4125)
                     /locus_tag="VFA02_07940"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HZU09821.1"
                     /translation="MQLPSLEEIHAATQPVRTVMPPTPQYTWPLLNARAGAEVWVKHE
                     NHSPVGAFKLRGAMVYIDWLRKDHPSVKGVIAATRGNHGQGVALAAARAGLTATIVVP
                     HGNSTSKNRAMQALGAKLIEHGDDFQASLEYATSLAKTDGLHLVPSFHPLLVHGTATY
                     AYEMLSNTPELDTIYVPIGLGSSICGVVAVRNAFRPRTKIVGIVDAQAPAYALSFREK
                     RPISHAANSRLADGLSCRVPVPEALEVILAHVDRIVEVSEPEIGAAMQAIYEDTHNIA
                     EGAAAAALAGLLKERESIAGKRVGFVLTGGNVDRNIFLEALQTA"
     gene            complement(4188..4964)
                     /locus_tag="VFA02_07945"
     CDS             complement(4188..4964)
                     /locus_tag="VFA02_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09822.1"
                     /translation="MPLKLSVALISWNADFAREHKRLLESANVRVALVISRGSGMIGK
                     ISKAAPSAIVVDLDAKPAYGREAAIVLRNSPSTRHIPLVFAGGSEEKVSLVRAEIPDA
                     VYCSWKTTRSGIERALVQQPARPVRPRRRGETVVHGSVLARKFDIKPDMPVAILGDVI
                     GLAERLSVVYEERISAQTLLVLFVVRTAYELDAAFECAAAQMPGSASFWIVYPKQSGA
                     LRTDFNQKDILALAHDNGFSGYKTCAVDEDWSAQKLTWRK"
     gene            4988..6355
                     /gene="miaB"
                     /locus_tag="VFA02_07950"
     CDS             4988..6355
                     /gene="miaB"
                     /locus_tag="VFA02_07950"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZU09823.1"
                     /translation="MADLQILDAPQKTPVSARPKTFYLETFGCQMNVHDSEKVIGTLV
                     QQGYRQVETEEDADLILYNTCSIRDKAEQKVFHRLNDFKKLTDQGKRFGVLGCVAQQE
                     GEHIFERAPHVSLVAGSASYRKLPEMLVRLELGDKKITGLDDRQTDETFETEFTVRSN
                     PHRGYITIIEGCDKFCAYCVVPFTRGKERSRTSNSVLAEARRMADAGYTEVQLLGQNV
                     NSYRDPEGKKSFAELLAAVGQVPGIRRVRFTTSHPRDFTRDIVEAIDAVPTLCDHVHL
                     PVQSGSSHVLKLMQREYTREWYLERISWIKAAKRQISMTTDIIVGFPGETPEDFEQTI
                     TLLHEVQYDGVFAFKYSPRPNTPALSMPDSIPDEVKSQRLQILLDRQREIQRANYSKH
                     IGQVLEVMVEGYNQARGQVVGRTSQNKTLNFTSSQPILPAAGSYLPVRVTKSFPNSLV
                     GEAVA"
     gene            6373..6858
                     /locus_tag="VFA02_07955"
     CDS             6373..6858
                     /locus_tag="VFA02_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZU09824.1"
                     /translation="MEIEMKIRGLMVDPSTNAPIVILKDVQGDTVLPIWVGLYEANAI
                     ALEVEKATTPRPMTHDLLKNVVQGLNASLQRVVVTELKDDTFYAVLWLEQDGETITID
                     CRPSDALALALRADCPIYVNEEVLRVAKVIPNPADQATQEELRRWLENLNDEDLGRYK
                     M"
     gene            6911..8431
                     /locus_tag="VFA02_07960"
     CDS             6911..8431
                     /locus_tag="VFA02_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09825.1"
                     /translation="MSLRRIGKLFAANNVGQAITVLTQLLLPPAFLHSFGVSLYGQWL
                     ALSAAISYLVTFNYGLQTYTSMQMTIHYNRGELQEAREIQSAGLRILFGALALFSAML
                     LVIFVIPINSWLHLTIPQREAQWTLYILGCQIVAGMIGGFFVNSYMVIGIPHRGQNLA
                     NLYQALVLFAQVALVLKHASFPAIAGAQLVLSLTSSIYLWIDVACLAPDIRPTLRYWK
                     KGSLGAILKPCGHYALLYSSNILAYQMPTILMQRLLGPASVVVFSVTRTIYSMTRRIL
                     NLLTNSIGPEITITFGQQNWRKLHKLYEFSERVILLLIAPITFGSMLATPLLLQVWLH
                     KGNLYQPALCILLGCTVSIQSIKEHKYQFQFSSNKVREISYMTVIAYSVQLLVAVPMM
                     IWLRLNGFTFTWLVTETIQLFYLLHLNTRLFGDHARVDRAPVYQLLGVMAVGCVVFFW
                     PVFHIAEFSYLVQGVVAVVAALLMLAVCYRLFQVDEVRSLLWEKVSKRLPAFASKR"
     gene            complement(8487..>9015)
                     /locus_tag="VFA02_07965"
     CDS             complement(8487..>9015)
                     /locus_tag="VFA02_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HZU09826.1"
                     /translation="VLFGDYNPAGRLTQTWVASMDQLPPMMDYDIRHGRTYMYLKTRP
                     LYAFGYGLSYTTFKYSNLRLSSPVLKKAGPITVTVDVTNTGRRAGDEVVQLYVTHLGS
                     KVERPIEELKGFQRISIQPGETKTISLPLKAQDLAYWDEKQHRFIVEDDTVQIRVGAS
                     SNDIRLEKALPVKAE"
BASE COUNT         1979 a         2384 c         2520 g         2132 t
ORIGIN      
        1 ggcgatgagg ggaaggagtt cgtatctcca cattccccac ttgtcatcct gagtggaaag
       61 cggagccgaa gaacatgttt ttctctttgc acggcgaaga aaaaccagga caagcaaaac
      121 ctgcgccaac agaaacagcg ctcccaacgc ggccaggttg aaccacatca gatggtcggc
      181 ggcggggccg tgcagagagc cgtccggagg catccaccac acgcggcctt cggtaagagc
      241 tgctaaatgg aaaccgtggg ccacgtgcat agtttcattg tcgctcagac ctgttgcttt
      301 tgctgttccc gcacgagatc aagaaagatc cgatggacgc ggcggtctga agaaagctca
      361 ggatgaaagg tcgcggcgag gatgtttttt tcgcggacga gaacggggaa gccatcgcgg
      421 tctgcaagca cttcgactcc cggcccggtc ttcactatgc gtggcgctcg gatatagacc
      481 atctccaatg ggccgccggg gattttggtt tcagctatct gaatgctgct gtcaatctgc
      541 cgcccataag cgttgcgctc tacagtggca tcgagaacgg ccaggctctc ctgcgatgga
      601 ttgcgaactt ctttggcaag cagaatgcat ccggcgcatg tgccgaaggt gggcttctgg
      661 cgcacgaagg cttgcagagc atcaaggaag ccatcgcggg ccagaaattt caggaatgtc
      721 gtagattcgc cgccggggat tacaagaccg tcaatgttgg ccagctcttc aggtttgcga
      781 acaaggacgg cttctgctcc ggcctcgcgc agggcctggg cgtgagcagc gtagtcgccc
      841 tggatggcaa gaactccgat gcggagttga gaggcatttc ccgatcctgg agtcaccctt
      901 tcttcctccg cgcatcttcc agaagtagct gctggatcag ttcgggagca gcaaaaaaac
      961 tcgtttcctg aagggccgcc aaagcagtag ggagatctaa gaggttgtga tacgaagctc
     1021 tcgtcagaat acccaaagta cctatgacct gaacatgaag ctgtgtggcg gccgttctcg
     1081 ccgccatatc atctaccagc aaataatctg cgtgcatttc gcgcgacagt gtaattgctt
     1141 cctgttcacc gggatcaaga tgggctattg agagattgat atgcgacgga catgtaacaa
     1201 cttcaaacca ttcaggaaga ttgtccatcc aggtacgaac cttctcaggg gcttgcacct
     1261 ctctcagctc ttctacaact ctctggggaa caagtacgcg tgagtaaagt tgtggcaaca
     1321 cgttaacatg cccaatctcg atcaaataat tgatgggtga agtatctgcg acaacaatca
     1381 tgcagaacgc ttctgctcat aagctgcggt aatatcttgt tcaaggtcct tcatcgagta
     1441 gttcagatgc accccatgtt ccttcagaaa ggcatggacc tgcattcttg tactgaaccc
     1501 gagtaaacgg cgcacctgac tttcagtaag actgcccgcc ctgtagccct ccagagccaa
     1561 tgcttccagt gctgcccgag aaagatcgga gtactgtgcc tgaagaacag tgccgacgtc
     1621 gtcaggaacc tgtaaatcaa aatgcatatt catggtgtct cctcagtcgc cctgctacca
     1681 acctctggtt tgcagcaatt gctcctgttc aatggctgct accgcaagac ctttcatggt
     1741 tccagtcacc tgctcggagg cctcggcgac aatcttcggg tcgttgtagt gtgtggttgc
     1801 gatgacaatc gcccttgcgc gggaaacggc ctcagcgcgc tcttccggat tgttctcaac
     1861 atcgagcgga gtggcgcgtt ctttcatgaa aattcccgag ccgacaaaga ccgactctgc
     1921 cccaagctgc atcatcaggg cagcgtctgc aggagtggca atgcctccgg cggagaagtt
     1981 gggaacaggc aacttgccgt tttttgccac catacggacc agttcgtagg gagcacgcag
     2041 ttccttggcg gcgacgtaca gctcatcgtc acggagcacg gtgagcgagc ggatatcgct
     2101 ggtgatctgg cgcatgtgct tgacggcgtg gacgacgtcg ccggttcctg cctctccctt
     2161 ggtacggatc atggcagcgc cttccgcaat gcggcgaagt gcctcgccaa ggtcgcgcgc
     2221 gccgcagacg aacggcgtgg tgaatgcgtg cttatcaatg tggttcgctt cgtcggcagg
     2281 cgtcagcact tcggactcgt caatgaagtc tacgccaatc tcctgcaata cctgggcttc
     2341 tgcaaaatgc ccgatgcggg ccttcgccat gaccggaatg gagacggcgg ccatgatctc
     2401 cttaatgagc tttggattgg ccatgcgggc cacgccgcct tcggcgcgaa tcatggcagg
     2461 gacgcgttcg agcgccatga cggagacggc cccggcttct tctgcgatgc gggcctgctc
     2521 gacgttcatc acgtccataa tgacgccgcc tttcaacatc tcggcgaggc cgaccttgag
     2581 gcgaagactg gtggtggaag tgccgttatt tgcgtactgc tctgtcatgg tttccctcat
     2641 accgcaacgc gaggaggtat atggcgtgcg gagaaaattg ttctgaactt attagtttag
     2701 cagttttgat agaccacgtt catgcacgaa gttgtgccag cttgttgtgg tccacaacaa
     2761 taaataaggc cttgctgcga gcaaggatcg tgccagatgc atcctgaatt tccgcttcac
     2821 aaaaattcct gcggccctga tgtgagctgc gccgcgcagt caatgtaagc ggttgtttca
     2881 gtggaacagg gcgcagatat tcaacttcta tctggtgtgt catcgctgtg gttccatgga
     2941 cgcgattggc tttgctcatg gcctcatcaa gaagcgtagc gatgatgcct ccatgggcat
     3001 gtccgggagg gccttcaaaa cgctgcgcca gccgcacgtg gcagacaatc tgctggtccg
     3061 catccacata gaacctgagc cgcaggccgg accggttttc tggtccgcag ccaaagcagc
     3121 ggttgagtgc tccgtgtgca agcggggtga gggtcttggt attcatgccg tttggagtgc
     3181 ttccagaaag atgttgcggt ccacgttgcc gcccgtaaga acaaagccaa cccgctttcc
     3241 ggcaatggat tcgcgttctt tcagtagtcc tgccagcgcc gcagcggcag cgccttctgc
     3301 aatattgtgc gtgtcttcat aaatggcctg catagctgct ccaatttccg gctctgagac
     3361 ttccacgatg cggtcaacat gggcaaggat cacttccaat gcttcaggaa ccggcacgcg
     3421 gcaggaaagt ccatcggcaa gacgggagtt tgccgcatgt gagatgggcc ttttttcacg
     3481 aaaagaaaga gcatatgcgg gggcttgtgc gtcaactatg ccgacgatct tcgtcctggg
     3541 cctgaacgcg ttgcgaacgg caacgacccc gcagatagac gagccgaggc caattggcac
     3601 gtaaatcgtg tcaagctctg gagtattgct tagcatctcg taagcgtaag ttgctgtgcc
     3661 atgtaccagt aatggatgga atgatggcac caggtgcagc ccatctgtct ttgcgaggga
     3721 cgttgcatat tccagcgatg cctgaaaatc atcgccatgc tcaatgagtt ttgctccgag
     3781 cgcctgcatc gcgcggtttt tcgaggtgct gtttccgtgc ggtacaacga tcgtcgccgt
     3841 aagccctgcg cgggccgcgg ccaatgcaac cccctgtcca tggttgccgc gggtagcagc
     3901 gatgactcct ttgacggatg gatggtcttt cctgagccaa tctatgtaga ccatggcccc
     3961 gcgcagttta aaggcaccga caggagagtg gttctcatgc ttgacccata cttctgctcc
     4021 cgcgcgcgca ttcagcagcg gccaggtgta ttgcggggta ggaggcataa ctgttcggac
     4081 gggctgagta gcggcgtgga tttcctcaag ggacggtagt tgcatgaaag aatgtctccc
     4141 gatggtggaa tattgaatag atgcggtcat ctgtggcaag gacgtagtta tttccgccag
     4201 gtaagttttt gtgcggacca gtcctcatct actgcgcagg tcttataccc ggaaaagcca
     4261 ttatcatgtg ccagcgcaag aatgtctttc tgattgaagt ccgtgcgcaa ggccccgctc
     4321 tgtttgggat aaacgatcca gaagctggct gatcccggca tctgcgcggc tgcgcactca
     4381 aaagcagcgt ccagctcgta tgccgtacga acgacgaaaa gtacaagcag ggtctgtgct
     4441 gaaatgcgct cttcgtatac gacggacaat ctttcagcca atccgatgac gtcaccaaga
     4501 atggcgaccg gcatgtccgg cttgatgtcg aatttacgtg ccagtacgga gccatgaact
     4561 acggtttcgc cgcgccgcct tgggcgaaca gggcgggctg gctgctggac caaagctctt
     4621 tcaattccgg agcgggtggt tttccatgag caatagaccg catccggaat ttcggcccgg
     4681 accagagata ctttctcttc tgaccctcct gcaaagacga gtggaatgtg ccgtgtagaa
     4741 gggctgttgc gcagaacaat ggcggcttcg cgcccgtaag ccggtttggc atccaggtca
     4801 accacgatgg ccgatggagc agccttgcta atctttccga tcatgccgga accgcggctg
     4861 atgacaaggg ccacgcggac atttgcgctt tccagcagcc gcttgtgttc gcgggcaaag
     4921 tctgcgttcc atgaaatgag ggcgacggaa agtttgagcg gcatagggcg atataatcaa
     4981 gataattatg gcggatttgc aaattctgga cgcgccgcag aaaacaccgg tcagcgcgcg
     5041 ccccaaaacc ttctacctgg aaacctttgg ctgccagatg aatgtgcatg actcagaaaa
     5101 ggtcattggc acgctggtac agcagggtta ccggcaggtg gaaacagaag aggatgccga
     5161 cctcatcctc tacaacacct gctctatccg cgataaagcg gagcagaagg tctttcaccg
     5221 tctgaatgat ttcaaaaagc tgaccgacca gggcaagcgc tttggtgtcc tgggatgtgt
     5281 ggcccagcag gaaggcgagc acatttttga gcgcgcgccc catgtttcgc tggtggccgg
     5341 atctgcgtca tatcgcaaat tgccggaaat gttggtccgt ctggaactcg gcgacaaaaa
     5401 aattaccggg cttgacgatc gccagactga cgagacgttt gagacagaat ttaccgtccg
     5461 ttcaaacccg caccggggat atatcaccat catcgagggc tgcgataagt tctgcgccta
     5521 ctgcgtcgtt ccatttacgc gcggaaaaga gcgcagccgg acttcaaact ccgttctcgc
     5581 cgaggcccgg cgcatggctg atgccgggta taccgaggtc cagctacttg ggcagaatgt
     5641 aaattcttac cgcgacccgg aagggaagaa gtcgttcgcc gaattgctgg cggctgtggg
     5701 ccaggtccct ggtattcgcc gtgtgcgctt tacaacctcg catccccgcg actttacccg
     5761 cgacattgtt gaggcgattg acgccgtgcc cacgctctgc gaccacgtgc atctgcccgt
     5821 gcaaagcggt tcttcacatg tgctgaaact gatgcagcgg gagtacacgc gcgagtggta
     5881 tctggagcgc atctcatgga tcaaggcagc aaagcgccag atcagtatga cgacagacat
     5941 catcgtcggt tttcctggag aaactccgga ggactttgag caaaccatta cgcttctgca
     6001 tgaagtgcag tacgacggcg tgtttgcctt caagtattcg ccgcgtccta atactccggc
     6061 actttccatg ccggattcga ttccggacga agtgaaatcg cagcggttgc agattttgct
     6121 ggaccggcag cgcgagatcc agcgcgctaa ctatagcaag catatagggc aggttcttga
     6181 agttatggtt gaaggctaca accaggcccg cggacaggta gtcgggcgca cgtcacagaa
     6241 caagacgttg aactttacat cgagccagcc tattcttcct gcggcgggca gctatctgcc
     6301 tgtacgggtg acaaaaagct ttccgaacag tctggtggga gaggcggtcg cgtaacaagg
     6361 aggaacatcc agatggaaat cgagatgaaa atccgcggcc tgatggtgga tccgtctacc
     6421 aatgctccca tcgtcatcct gaaagatgta cagggcgata cagtgctccc aatctgggtt
     6481 ggcctctatg aagccaacgc cattgcgttg gaagtcgaaa aggcgaccac gccgcgaccg
     6541 atgacgcacg acctgctgaa gaacgttgtt cagggcttga acgcatcatt gcagcgcgtg
     6601 gtggttacag aactgaagga cgacaccttc tatgctgtgc tttggctgga gcaggacggc
     6661 gagaccatca cgatagattg ccgtccgtcc gatgcgttgg cccttgctct gcgcgcagac
     6721 tgccccatct atgtaaatga agaagtgctg cgtgtggcaa aagtcattcc taatcccgca
     6781 gatcaggcga cgcaggagga gttgcgtcgc tggctggaaa acctcaacga cgaggatctg
     6841 ggccgctaca aaatgtaact ggtcttttgc ccttcgaaat ggagtaaagt gtccctgatg
     6901 ggcacgcccc atgagtctac gccgcattgg aaagcttttt gcagccaaca acgtgggaca
     6961 ggcgatcact gtcctcactc aactgctttt gccgcccgcc tttctgcata gttttggcgt
     7021 aagcctctat gggcagtggc tggcgttgag cgcggcaatt tcttatcttg ttacctttaa
     7081 ttacggcctg caaacctata ccagcatgca gatgacgatc cactataacc gtggagagtt
     7141 gcaggaagct cgggaaatcc agtcggcagg gttgaggatt ttattcgggg cgctcgcatt
     7201 attcagcgcc atgctgctgg tcatctttgt cattccgatc aactcctggt tgcatctgac
     7261 gattccgcaa cgggaggccc aatggacgct ttacatcctg ggctgtcaga ttgtagccgg
     7321 aatgattggc gggttctttg tcaacagtta tatggtcatc ggcattccgc atcgtggaca
     7381 gaacctggcc aatctgtatc aggcgctggt gctgtttgcg caagtggctc tggtgttaaa
     7441 acatgcttcg tttccagcca tagccggggc acagcttgtg ctcagcctca caagttcgat
     7501 ctatttgtgg atagatgttg cctgccttgc tcctgatatc cgccccacgt tgcgttactg
     7561 gaaaaaagga tctctggggg ccatcctgaa accctgcggt cattatgctt tgctatattc
     7621 ttccaacatt ctggcttatc aaatgccgac catccttatg cagcgcctgc tggggcctgc
     7681 ttcagtagta gttttcagcg tgacacggac aatctattcg atgaccagaa gaatcctgaa
     7741 ccttctgaca aattcgattg gtccggaaat tacgatcaca tttggccagc aaaactggcg
     7801 caagctgcac aaactgtatg aattttctga gcgggtcatt ctgctgttga ttgctcctat
     7861 caccttcggc tccatgctgg cgaccccact tctgcttcag gtatggctgc acaaagggaa
     7921 tctttaccag cctgcactct gcatattgct ggggtgcacc gtttctatcc agagcattaa
     7981 ggagcacaaa tatcagttcc agttttccag caacaaagtg cgcgagattt catatatgac
     8041 ggtcatcgct tactccgtgc aactgctggt ggccgttccc atgatgatct ggttaagatt
     8101 gaacggattc acttttacct ggcttgtcac tgagacaatc caacttttct atctgttgca
     8161 tttgaataca aggctttttg gagaccatgc gcgggtggac cgcgctcccg tgtatcagct
     8221 tttgggtgtg atggctgtgg gatgcgttgt cttcttctgg cctgtttttc acattgcgga
     8281 gttttcttat ctggtgcagg gtgtagttgc agtagtagct gctttattga tgctggccgt
     8341 atgctaccgg ctctttcagg tggatgaagt acggtcgctt ctgtgggaaa aagtcagcaa
     8401 acgcctgcct gcatttgctt cgaaacggta ggagcagcag tgcgggtctg ttcagaagta
     8461 cggtcagcca caacaggcct tcagcttcat tccgccttta ccgggagcgc cttttccagt
     8521 ctgatgtcat tggaggatgc tccaactcta atctggaccg tatcgtcttc cacaataaaa
     8581 cggtgttgtt tttcatccca gtaagcaagg tcctgtgctt tcagtggcag actgatggtc
     8641 tttgtctccc caggctgtat ggagattcgc tggaagcctt tcagctcctc aattggacgc
     8701 tctaccttag agccaagatg ggtcacataa agctggacga cttcatctcc ggcgcgtctg
     8761 cctgtatttg tcacatccac agttacagtt atggggccgg ctttcttcag cacaggagaa
     8821 ctcaggcgca ggttgctgta tttgaaggtt gtatagctaa ggccgtatcc gaaagcatag
     8881 agcggcctgg tcttcagata catgtaagta cgtccgtggc ggatgtcgta gtccatcatg
     8941 ggcggaagct ggtccatgga ggcgacccag gtctgtgtca ggcgtcctgc agggttgtag
     9001 tcaccaaaga gaacg
//
LOCUS       DASRBV010000210         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_726741, whole genome shotgun sequence.
ACCESSION   DASRBV010000210 DASRBV010000000
VERSION     DASRBV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726741"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFA02_07970"
     CDS             <1..826
                     /locus_tag="VFA02_07970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZU09827.1"
                     /translation="NACHEKNTIANALFGAKPLDPAKMYDEYARAAEQIAPFVTDTAA
                     LLNKTIAEGGSVMFEGAQGTMLDIDHGTYPFVTSSSATAGGAVTGTGVGPTKIGTVIG
                     VTKAYVTRVGEGPFPTEIFDGAGETLRARGQEFGAVTGRPRRCGWLDLPLLRYSNQIN
                     GTEWLVVTKLDVLDALEEIPVCTGYKINGKLVDTMPADVRGLEAIEPVYTKLKGWASS
                     TEGITEFEKLPKLAQEYLHFLEKESGAKTGMISTGPDRDQTMLLPEFATALKEIRS"
     gene            855..1106
                     /locus_tag="VFA02_07975"
     CDS             855..1106
                     /locus_tag="VFA02_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09828.1"
                     /translation="MAHKAGTSDADNGLAHATNDIGLISLPGGRKLAIAVFITGSTAG
                     DATREGMIARMAQAAYESAVAASRKWPLPSTQVLSYFQA"
     gene            1109..1396
                     /locus_tag="VFA02_07980"
     CDS             1109..1396
                     /locus_tag="VFA02_07980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZU09829.1"
                     /translation="MVSFTVRMRFAEKDREKIAEILRELAPASRQEPGCVTYVPHWVD
                     GDACTVLIYEQYKDEAALEAHRATPHFQQYAIGGLYQMMLERSVENLVAMI"
     gene            1558..2082
                     /locus_tag="VFA02_07985"
     CDS             1558..2082
                     /locus_tag="VFA02_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU09830.1"
                     /translation="MRRHFRVRTPLSAMNGAGFLLFLLALSGLAVARPAFASLPHRGG
                     HELHKEIEALEMQWRQAQLNNDVGLMDRLLAEDYIGISANGTIETKAEALALRRAGTL
                     HITTLDLNDLKVRIYGDTAVVTSRAYVTGTNGSSNISGQYRYTRVYNRRFGQWKIVSF
                     EASRMHDVGEREKH"
BASE COUNT          503 a          639 c          646 g          444 t
ORIGIN      
        1 gaacgcctgc cacgaaaaga acaccatcgc caacgcgctc ttcggcgcca agccgctcga
       61 tccggcaaag atgtacgacg agtacgcaag ggccgccgag cagattgctc cttttgtgac
      121 cgacacggcg gcgctgctga acaagaccat tgctgaaggc ggcagcgtga tgtttgaagg
      181 cgcgcaaggc acgatgctcg acattgacca cggaacgtat cccttcgtca cgtcctcgtc
      241 ggccaccgcg gggggagcag tcaccggaac gggtgtcggg ccgaccaaga ttggcactgt
      301 cattggcgtc accaaagcct atgtaacacg cgtaggcgag ggaccgttcc cgacagaaat
      361 ctttgatggg gcgggtgaaa cgcttcgtgc gcgtggacag gagtttggcg cggtgacagg
      421 gcgtccccgg cgctgtggat ggcttgacct tccgctgctg cgctactcga accagatcaa
      481 tgggaccgag tggctggtgg tcaccaagct cgacgtgctc gatgctctcg aagaaatccc
      541 ggtatgcacc ggatacaaaa tcaatggcaa gttggtggac accatgcctg ccgatgtccg
      601 cggccttgag gccatcgagc cggtttacac gaaactgaag ggctgggcat cttcgactga
      661 aggcatcacg gaattcgaaa agctgccaaa gctggcgcaa gagtatctgc acttccttga
      721 aaaagaatcc ggcgcaaaga cggggatgat ctccactggt cctgaccgcg accagacgat
      781 gctgttgccg gagttcgcaa ctgcgctgaa agaaatacgc tcgtaatcat gaatgtctac
      841 ccgagggtgc ggttgtggca cataaagcag gcacatccga tgcggacaac ggcctggccc
      901 atgccacaaa cgatattgga ttgatttcat tgccgggcgg acgaaaactc gccattgctg
      961 tttttattac cggttcgacg gccggcgatg caacgcgcga aggcatgatt gcccggatgg
     1021 cgcaggcggc ttatgagtct gctgttgctg cttcgagaaa atggcccctt ccctccacac
     1081 aggttttatc gtactttcag gcatagatat ggtcagtttt acagttcgaa tgcgttttgc
     1141 cgagaaagac cgcgagaaga tcgccgaaat tctgagagaa cttgctccgg cctcacgcca
     1201 ggagcctggc tgcgtcacct acgtccctca ctgggtagat ggggacgcct gcacggtgct
     1261 catttatgag caatataaag acgaagctgc actggaggcg caccgggcga ctccgcactt
     1321 tcagcaatat gccatcggcg ggctgtacca gatgatgctg gagcggtcgg tggaaaatct
     1381 ggttgctatg atctgaacct ttaggagcac cacagaagct gcgcgctctg cgtccaagaa
     1441 aatatgttgg ccggtttcca tccagtccgc cggattccgc attcagggcc agaaatggcc
     1501 tgtctgggct ttctttttga ctcgcggcgc agaaccggac taacatccga tacgcacatg
     1561 cgcagacact tccgcgtccg taccccgctt tcagcaatga atggcgcagg ttttttgctg
     1621 tttcttctgg cgctttcggg actggctgtg gcacggccgg catttgcttc tctgccgcac
     1681 cggggcgggc acgagctgca caaagagatc gaagccctgg aaatgcagtg gcgccaggca
     1741 cagctaaaca acgatgtagg attaatggac cgcctgcttg ccgaagacta catcggcatc
     1801 agcgcgaatg gcaccatcga aacgaaagct gaagcgctcg ccctccgccg cgccgggacg
     1861 ctgcacatca ccacgctcga tctgaatgac ctgaaagtac gcatctatgg ggacactgcg
     1921 gttgtgacct cgcgcgccta tgtgacggga acaaacggat caagcaacat cagcggacag
     1981 taccgctata cccgcgtcta caaccgcagg ttcgggcagt ggaagatcgt cagctttgaa
     2041 gccagccgca tgcacgacgt aggcgagcgg gaaaagcact gatggtggcc taaaacggcc
     2101 tggcgcccga aactaccgct gcctgacccg accgctgcac ccatctgccg ttgtgttttt
     2161 ccagataaat ccactccccc tggctgcata catttccgca ccagtcgagt tgatagacca
     2221 gtgcggccct cc
//
LOCUS       DASRBV010000211         7597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_367114, whole genome shotgun sequence.
ACCESSION   DASRBV010000211 DASRBV010000000
VERSION     DASRBV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367114"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4453)
                     /locus_tag="VFA02_07990"
     CDS             complement(<1..4453)
                     /locus_tag="VFA02_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZU09831.1"
                     /translation="MKYLLQNDDMVLLQVKQDYQTTTTSVVSYDTNNSHLGNSQTTTI
                     GVSTSSGTAFDPRTTFAASEAAGRMYNTNTDTIAVLHAIDDYNPAWQITLFDPQTRSA
                     NTTRLNTSFRPYGTIFTQIVMGDFNGDGLADPLVFYLCLFNNSTQWGMKVLTAADPKT
                     TGSLTEGPEFYGNSEPLPLAAGSRVIAVGDFNGDGRDEIAMLLADHQNIAFYQVDPKT
                     LQISPVPDPSNNNQPLTVKLTGLNGAAVTMLSNRVTLVAGRFRQCGATGNPCSPNGIN
                     NADLVVFGQIYSISGVHTGGCSNDVPCGYSVIPIQITPNYNNNGAPSGTFTAQVVPQS
                     SPSPETPFFRFPDYHGASGALAQAAPLVNWPQQTNEQLVLGIRTNDGASYIEIGTFVN
                     DGTLDQFDWESETEREFDAAYTQLQNLWVGNFDNQDSGAHSSKLQIETYEVVAYDFGL
                     FLTPHINIFDVNAPMPFHVPVDHRTNWLKQVSGYDSGVPLGPGMDFTSITNDPPGQNF
                     LVPADIQGRSLRLGSPTIVRVTSQIQPDVVLAMPPMHADWIKPYGVNPPYAPSFQNCT
                     DSNDPCLVNVSVLPSVEPSYGKPFATLFNFDSGTKSSAVQSSTTSWGVSVKVSEGASF
                     SFNDGLENASGSFTNTTKTAHDETVKKAYDTYSGWSNSLNVTTGFSDYLRWTQKDVNV
                     YYYPVLGCGSNNADCPDNRTYVEFSVPDHVQYWLGDSAKQEWYQPVHEPGNLLSYPWS
                     KSQLEKRFTDSLDSLSSINPPWASFGTSESSFNTTWSKSSVQSQTSGSTSSFSDEISM
                     SYSEGMGVGGIDSADINFNTDINAHTSLDTLNESTLSLSSSSGVTLNIPGFEDTSGCC
                     SYGFSQYIFGLENKNYTPGENACGAAKQGVAKSCIPVTDPDQGQQVAVATIGPLFTAY
                     VADPYEPANGNNAAWWQNVYTKPDVALNHPERWKWNKATRTVSLNQPDFSGNTSPVQQ
                     QFYHMKGFFISKAPLTLNSGPNLAMAGASDDLTLTTRVYNYSLVDTTAPVHVRFYGQL
                     YCSSSDNNHENQEASCRDWKTGQECNAGNLCGDSFQIGQDQIIPFIAGFNDNGNSDMP
                     NWQTTSVDFQPVKFPATKNGNVHMVFWVVVWMQDNSGNLVAEMQDHGLTTIPDPGVTQ
                     ITQVHVENYSNNVGMYPVHAPFFITPAGMLSGAIQGGGALQSIAITADPHVQLEQNTK
                     IEASLQATGAPVKSVNIAYYDGDPAHNGTLLDTQKIVYMDPGRNYYHWTSFAPDTCGI
                     HTLYAKAWTANSPAVQASTITSVTLDAVGFTQTLITATQGAKISDTSLRNTLLSLLNS
                     ALQFFEQGNAQAGDNALGLYLQQVSSTNGITPASASRLSGQASVLLGCGPSGFSLSAL
                     PSSFTVTAGLPASYPLAITPIGGFNGAVALSCVGLPAGAECSFTSPQVTLDGTTQSRV
                     TLTITTISRSASAAGIMTGPPLSGRGKWTWFLMLFISALAIAELHRGR"
     gene            5007..7055
                     /locus_tag="VFA02_07995"
     CDS             5007..7055
                     /locus_tag="VFA02_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09832.1"
                     /translation="MEEFLVCPAKTISEYQLGFEVFERSTDFDPQVDSVVRVQVARLR
                     QKLAQYYAEEGRIDRILIEVPRGGYAVSVTFREISQPTLPLDSDSKPHRPLFPRFGWI
                     AGIMVLLAFTAFAIFRWPVVQEAKSSSEATVVPFTSDPGLQDDAAFSPDGTSVAFSQS
                     SEGGREIFIKQIRNGQTSQLTHDKGIDINPAWSPDGSAIAYYRVKQDGGEFVIVPLQG
                     GATRVAGDSWVGIPGTATASSSMPDAGPAWTKDGNHLVIADKNGNGGKDCLFVLNIQD
                     GTRRLLTSPPVTEIGDGSPAVSPDGKTVAFIRKATSYGLGDLFLVPLEGGQPRRMTFD
                     NADMNGAAWTADGRQIIFSSNRGGSHRLWRIDMATGQVASFAASGLNSLRPAIDPTGD
                     RLIYTEWTTNTNIWRFDLKAPLPERKGTVWIQSTLHQDSLQYSSDGRHIAFISDRSGF
                     WELWMADADGENMRQLTHFGGPQAGSPQWSPDGKEIAFDLRGDGVSNIFVVDVSTGTI
                     RQVTHDSSDTMVPKWASDGRSLYVASRRTGQLQIWKISLDGSLWTQLTRNGGMDGLEV
                     PGKDELVYTLLESPGFWILDLRTGKESVIPALANVRSYRYWALSPDGIYYVSGNNKNT
                     RYPVNFYDFTTHHITPIFEIERPLYSGTPSLSVSPDGHYLAYAQVDSFHANLVLAENW
                     "
     gene            7221..>7597
                     /locus_tag="VFA02_08000"
     CDS             7221..>7597
                     /locus_tag="VFA02_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HZU09833.1"
                     /translation="MTQQADKVEEVQVAAEAIPAAWLKTELARHPQRPYPMDFIKSIF
                     TDFSEIHGDRAFGDDPAMCCGMARFDGEEVMVIGNLKGRTVKERVQRKFGSPEPEGYR
                     KALRAMRMAEKFGRPVFTFIDLAG"
BASE COUNT         1833 a         1899 c         2046 g         1819 t
ORIGIN      
        1 cacgtccgcg gtggagctcc gcaatcgcca atgcggagat aaacagcatc agaaaccagg
       61 tccattttcc ccgccctgaa agtggggggc cagtcattat tcctgccgca gaagcagagc
      121 gggaaattgt ggtaatcgtg agcgtgaccc gcgattgtgt ggttccatcc agagtcacct
      181 gtggcgaagt aaaggaacac tccgccccag ccggcagacc aacacaactc agcgcgaccg
      241 caccattgaa tccgccgatg ggcgtgattg ctaatggata ggaggccggc agtcctgctg
      301 taactgtgaa cgaagacggc agggccgata gtgagaatcc gctggggcca caacccagca
      361 gcacactggc ctgcccgctg agacggctgg cacttgccgg ggtgattcca tttgtagacg
      421 agacctgttg caaataaagg ccaagagcgt tgtctcccgc ctgagcattg ccctgctcaa
      481 agaactgcaa tgctgaattc agcagggaga gcagggtatt gcgaagcgaa gtgtcgctga
      541 tctttgctcc ttgcgtcgca gtgatcagag tctgcgtaaa accgactgca tccagagtga
      601 cgctggtgat cgtgctggcc tggactgctg gcgagttggc agtccacgcc tttgcataaa
      661 gagtatggat gccgcatgtg tctggagcaa aagaggtcca gtgataatag ttcctcccag
      721 gatccatata tacgatcttc tgtgtgtcca gcaatgttcc attgtgggcg gggtctccgt
      781 cgtaataagc aatgttgacg gattttaccg gtgcgcctgt tgcttgcagg ctggcctcga
      841 ttttcgtgtt ctgttccagt tgcacatgag ggtcagcagt aatggcaatg ctttgcagtg
      901 cccctccgcc ctgtattgct cctgataaca tgcctgccgg agtaataaag aaaggagcat
      961 gcaccgggta catgccaacg ttgttactgt agttttcgac atgcacctgt gtaatttgcg
     1021 taacgcctgg atcgggaata gttgtcagcc cgtggtcctg catctcagca acaaggtttc
     1081 cgctgttgtc ctgcatccag accacaaccc agaagaccat atgaacattg ccgtttttgg
     1141 tggccggaaa tttaacaggc tgaaaatcta cgctcgtggt ctgccagttg ggcatgtcgc
     1201 tgttgccgtt gtcattgaag ccggcaatga atggaataat ctggtcctgt ccaatctgaa
     1261 aactgtctcc gcacaaattg cctgcattgc attcctgtcc ggtcttccag tccctacagc
     1321 tcgcttcctg gttctcgtgg ttgttgtcgc tggaactgca ataaagctgg ccgtagaagc
     1381 gcacgtggac aggagcagtt gtatctacca gactgtagtt gtagacgcga gtggtcaggg
     1441 tcagatcatc actggcgccg gccatggcaa gatttggccc tgagttaagc gtaagggggg
     1501 ctttgctgat gaagaaaccc ttcatgtgat aaaactgctg ctgcaccgga gatgtattgc
     1561 cggaaaaatc tggctgattc aggctgaccg ttcgtgtagc tttgttccat ttccagcgct
     1621 cagggtgatt gagagcaaca tccggtttcg tataaacatt ctgccaccat gcagcattgt
     1681 tgccatttgc aggctcatac gggtcggcga catacgcagt gaacagcggc ccgatggtgg
     1741 caacagcaac ctgttgccct tgatcgggat cagtcacagg gatacaactc ttcgccactc
     1801 cctgcttagc agcgccacat gcattctcac ctggagtata gtttttgttc tcgagaccaa
     1861 agatgtactg gctgaatcca tagctgcaac aaccgctcgt atcttcaaaa ccaggtatgt
     1921 tcagagtaac tccgctggaa gaactcaaag aaagcgtaga ttcgttcaaa gtatcaagcg
     1981 aagtatgggc gttaatgtcc gtattgaaat ttatatcggc agaatctatg cctcctactc
     2041 ccatgccttc ggaatagctc atggaaattt catcggaaaa ggaactggtt gagccactgg
     2101 tctggctttg aacgctggat ttgctccatg tggtgttaaa gctcgattcc gaagtaccga
     2161 agctggccca gggcggatta atcgagctca gtgaatcgag ggaatccgtg aaccgttttt
     2221 ccagttgcga tttgctccac ggataagaga gcagattacc tggttcatgg actggctgat
     2281 accactcctg tttagcactg tcacccagcc agtactggac atgatcagga acagaaaatt
     2341 ccacgtaggt acgattgtca ggacaatctg cattattgga accgcatccc agcaccgggt
     2401 agtagtagac gttcacatct ttttgtgtcc aacgaagata atcgctgaag ccggtcgtta
     2461 cattcaggct attggaccac ccggaatagg tatcgtaggc tttttttacg gtttcatcat
     2521 gagcagtttt cgttgtgttt gtaaagctgc cactcgcgtt ctccagacca tcattaaatg
     2581 aaaaacttgc tccttcggac accttcacgg aaacgcccca gcttgtggtg ctggattgga
     2641 ccgcagaact ctttgtgcca ctatcgaaat taaagagagt ggcgaaaggt tttccataag
     2701 aaggttccac actgggaagc acggaaacat tcaccaggca tggatcattc gagtcagtgc
     2761 aattctgaaa agatggagca tagggcggat taacaccata aggcttgatc cagtcagcat
     2821 gcataggcgg catggccagg acaacatcag gctggatctg cgatgttacc ctgacgatcg
     2881 tgggcgatcc cagacgcaga gagcgaccct gaatatcagc aggaacgaga aaattctgcc
     2941 ctggcggatc attggtaatg ctcgtaaaat ccatccctgg tcccagcggc actccggaat
     3001 catatccgct tacctgtttg agccagtttg tcctgtggtc tacaggtaca tggaatggca
     3061 tgggcgcgtt aacatcaaaa atgttgatgt gaggagtcag aaaaagacca aagtcataag
     3121 ccaccacttc ataggtctca atctgtaact ttgaactgtg cgcacccgaa tcctggttat
     3181 caaagttccc aacccacagg ttttgtagct gagtatatgc ggcgtcaaac tctcgctctg
     3241 tctccgattc ccagtcgaac tggtccaggg tgccatcatt tacgaaagtg ccgatctcaa
     3301 tgtaacttgc gccatcatta gtccgaatgc cgaggaccag ttgttcgttt gtttgttgtg
     3361 gccaattgac aaggggcgca gcctgcgcaa gtgcgccgct ggccccatgg taatcaggaa
     3421 accggaagaa cggggtctct ggcgagggac ttgactgtgg cacaacctgg gcggtgaagg
     3481 tcccgctggg ggccccatta ttgttgtaat ttggcgtgat ctggatgggg attacgctat
     3541 atccgcaagg cacgtcattc gagcagccgc ctgtgtggac gcctgaaatc gaatagatct
     3601 gcccaaagac aaccagatcg gcattgttga taccgttggg agagcaagga ttgccagtcg
     3661 ccccgcactg acggaaacgt ccggcaacga gcgttacccg attagagagc atggtgaccg
     3721 cagccccatt cagtccggtc agctttacgg tcagaggctg gttgttgttg ctggggtcgg
     3781 gcactggact gatctggaga gttttcggat ccacctgata gaaagcgatg ttttgatgat
     3841 cagccaacaa cattgcgatc tcgtctcgtc cgtcgccatt aaaatcgcca accgcgataa
     3901 cgcggctgcc tgctgcgagc ggcagcggct cagagttgcc gtaaaattca gggccctccg
     3961 tgagcgaacc cgttgtcttt gggtcggcag cggtcagcac cttcattccc cattgggtcg
     4021 agttgttgaa aaggcagaga taaaagacca gtggatcagc aagtccatcg ccattgaaat
     4081 cgcccataac aatctgtgtg aagatcgtgc cgtatgggcg aaaactggtg ttcaaccgtg
     4141 ttgtgttcgc agatctggtt tgcggatcga agagggtaat ctgccaggca ggattgtagt
     4201 catcaatcgc atgaagtacg gcgatggtat ccgtgttggt gttatacatc ctccctgctg
     4261 cttctgacgc cgcaaatgta gtccttggat cgaacgcggt cccggaagaa gtagatacac
     4321 caatcgttgt agtttgcgag ttgccaagat gggagttgtt ggtatcataa ctcacaaccg
     4381 atgtagtagt cgtttggtag tcctgtttga cctgcagcaa taccatatcg tcattttgga
     4441 gaaggtattt cattcctccc gtgacatcgg tgactccgga catatcatgt gcgccgctat
     4501 cggatgtctg tgcgtgggcc gcgggacaca gcatccagac gaaaagtgca tgtatggcca
     4561 gctttccgac aaactgaagg taagaggggc aactttccca agtcagtctc atatctcctc
     4621 caaggcaggg tccctgcatc ggcacagttt aagtggctta taaagtatgt gtttttagcc
     4681 atttccgcga aggatgatac ccgcggagag ccaaataaga tcgggggaca taagtgcctc
     4741 aaatactatg gagaagggga aaaaagagaa gttacatcat gttacacagc atgcgtaact
     4801 tctctccgaa gaggcaaaat tttatagaaa tttaaattac ttacgttgta acataggggc
     4861 gcgacttgac tcgtagataa aaggaacttt gctgaacggc tgcccccatg tcgcgcgcgg
     4921 aagaaatacg ggcccaactt gaacggattc tgacatcccc ggaattccat tcctcccagc
     4981 gcctgagccg gctgctccaa tatctcgtgg aagaattcct tgtctgtcct gcaaaaacca
     5041 tcagcgaata tcagctcggc tttgaagtgt ttgagcggtc caccgatttt gatcctcagg
     5101 tagattctgt tgtgcgtgta caagtagccc gattgagaca aaagctggcg caatattatg
     5161 cggaagaagg aaggattgac cgcattctca tcgaggtccc ccgcggcgga tatgcggtta
     5221 gcgtaacttt ccgtgaaatt tcacaaccta ctctccctct cgattctgat tccaaaccgc
     5281 atcggccgct gtttcctcgt tttggatgga ttgccggaat catggtgctg ctggcattca
     5341 cagcatttgc tatcttccgc tggccggtgg tgcaggaagc gaagtcgtcg tcagaagcga
     5401 cagttgtccc cttcacgtct gatccgggat tacaggacga cgcagctttt tctccggatg
     5461 gaacatctgt ggcctttagc caatcaagcg aaggcgggcg agaaatcttt attaaacaaa
     5521 ttcgaaatgg gcaaaccagc cagcttaccc atgataaggg tattgatatc aatcccgcat
     5581 ggtcgccgga cggatcagcg atcgcctact atcgagtgaa acaggatggc ggcgaattcg
     5641 tgattgtgcc gcttcagggc ggagcaacac gtgtggcagg ggattcctgg gtggggattc
     5701 caggtactgc gaccgcttcc agttccatgc ccgatgctgg tcccgcatgg accaaggacg
     5761 gcaatcacct ggtcattgct gacaaaaacg gaaatggtgg aaaagattgc ttattcgttc
     5821 tcaacataca ggatggtacg cgcagactat taacttcacc cccggtaaca gaaataggag
     5881 acggctcacc tgcggtgtcg ccggatggaa aaactgtcgc atttattcgc aaggccacat
     5941 cgtatggttt aggagatctt tttcttgtgc cgctcgaagg cggccaaccc cgccgcatga
     6001 ccttcgataa tgccgacatg aatggagcag cctggactgc ggatggccgc cagatcatct
     6061 tttcgtctaa ccgcggaggg agtcatcgtc tgtggcgaat tgatatggca actggccagg
     6121 tcgcctcatt tgcagcatca gggctgaatt cactgcgccc cgcgattgac cctaccgggg
     6181 accgcctgat ctataccgaa tggaccacca ataccaacat ttggaggttc gatctgaaag
     6241 cgcctcttcc agagagaaaa ggaacggtat ggatccagtc cacgctgcat caggacagct
     6301 tgcaatattc atccgacgga aggcatatcg cattcatttc cgaccgttcg ggattctggg
     6361 aactctggat ggccgatgct gatggagaaa acatgcgcca gttgacccac tttggcgggc
     6421 cacaggctgg cagtccgcag tggtctcccg atggaaaaga aatcgcattt gatcttcgcg
     6481 gagacggggt ctcgaatatt tttgtagtgg atgtaagtac aggaaccatc cggcaggtta
     6541 cacatgatag ttctgacaca atggtcccga aatgggccag cgatggacgg tctctttacg
     6601 ttgcatcgcg aaggaccgga caactccaga tatggaaaat ctctttggac gggtcgctat
     6661 ggacccaact gacgcgcaat ggaggcatgg acggactgga agtcccagga aaggatgaat
     6721 tggtatatac gctacttgag agtccgggat tctggattct ggatctccgg acaggcaaag
     6781 agtcggtcat tcctgcgctg gcaaacgtca gaagttaccg ctactgggcg ctctcgcctg
     6841 atggtattta ctacgtttca gggaacaaca aaaacacacg gtacccggta aatttttacg
     6901 atttcacaac gcatcacatc actcccattt ttgaaatcga acgccctctt tacagcggca
     6961 ctccaagtct ctctgtttcg cccgatgggc attaccttgc ctatgctcaa gtagattcgt
     7021 tccatgccaa cctagtgctt gcagaaaact ggtagattca gaatgcctcg gcccgggtgc
     7081 tgcccacttc gagtggatta ccagatgaag ccgcctgtgg attgggcctg ataaagtaac
     7141 catgctctgg ttcaaaaatc gtggcgccat ggtttacatc ctctgccatg tacaatcaga
     7201 cagtaagact tgcagcgtct atgacacagc aggcagacaa agtagaagaa gtgcaggtgg
     7261 cggccgaggc cattcctgcg gcgtggttga aaacggagct ggcgcgtcat ccccaacgcc
     7321 cgtatccaat ggactttatc aaaagcatct tcactgactt cagcgagatc catggcgacc
     7381 gtgcctttgg cgacgatccg gcgatgtgct gcggaatggc gcgctttgat ggggaagaag
     7441 tcatggtcat cggcaacctg aaagggcgca cggtgaagga gcgcgttcag cgtaaattcg
     7501 gctcgcccga gccggaaggg taccgcaagg cgcttcgcgc catgagaatg gcggaaaaat
     7561 ttggccgtcc ggtcttcact tttattgatc tggccgg
//
LOCUS       DASRBV010000212         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_677075, whole genome shotgun sequence.
ACCESSION   DASRBV010000212 DASRBV010000000
VERSION     DASRBV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677075"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFA02_08005"
     CDS             complement(<1..1454)
                     /locus_tag="VFA02_08005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU09834.1"
                     /translation="MQNTWQDILILGLLVLLFGMIHRKRTSPRLLFWIAGWIFVLFHF
                     AALEPHPSTYLWQCVQGAISTAGLVLSGACFLLASTVTRLSKRHLFLIGAISSIPALI
                     YIFLVSFDYLRVAPLVLLVNTSECAVIWAGSRFARKRKKVLWANNLIAIACGTWLLIL
                     IDHGRPELGLNAVLTQIFLMNAVLFREDFRRVSVGVITASVGLVAWAMVFPAGLFTDH
                     FFPHLVISPNLWNVPKYFVEFGMILTLLEEQIVDAETQREQYQLLFDSNPHPMFIYDP
                     GSSRVLRVNDAALAQYGYSREEFLSLNALDLIEMADRSEAQKVMSSRPGEAVRTTGPW
                     KNRRKDGSRFYADISSHFIEFAGRKARFCMIIDVTEKQRLYEQLEHRANHDSLTNLPN
                     RFHLEQRLRHTLESAARHNQKAAILCIDLDRFKQINDTYGHAVGDICLQEVAGRLKKR
                     LRESDTVARTGGEEFTVVLGDLHSDEDAEKVATDL"
     gene            complement(1608..1684)
                     /locus_tag="VFA02_08010"
     tRNA            complement(1608..1684)
                     /locus_tag="VFA02_08010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1648..1650),aa:Val,seq:tac)
     gene            1790..2635
                     /locus_tag="VFA02_08015"
     CDS             1790..2635
                     /locus_tag="VFA02_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZU09835.1"
                     /translation="MRRLIVNADDFGLTSGINRAVTELHSARALSSATLMATAEAAPF
                     FQAVGETRNQPSLGVGCHIVLVDGTPLLPPSQVPSLVDGAEFRPKLGAFVTDLLLGRI
                     AEADIEAEAVAQIRKLQQAGVPVTHLDTHKHTHTFPRVLLPLLRAAQQCGVKAIRNPF
                     EPDWALNATANAGHVRKMQVRLLRSQSRAFAQQVNRAGLVTTDGAIGVLATGTLDTQT
                     VQNLLAALPEGTWELVCHPGYNDADLKKQRTRLLESREVERTALLETVPAAQVELVHF
                     GQLSP"
     gene            2731..3903
                     /gene="bshA"
                     /locus_tag="VFA02_08020"
     CDS             2731..3903
                     /gene="bshA"
                     /locus_tag="VFA02_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZU09836.1"
                     /translation="MKIGITCYPTYGGSGVVATELGIELARAGHQVHFITYSQPFRLT
                     GREEGICYHEVPVSNYPLFEYPPYDLALASRMAEVAEYYGLDLLHVHYAIPHSVSALL
                     ARQMLATRGRHLPFVTTLHGTDITLVGLDRSYLPITQFSIEESDGITSISQYLKDRTI
                     KEFKTKNEIEVIPNFVNCDVYVPLPGEIRQEERARYAAPGEKLLIHLSNFRPVKRVTD
                     AIEVFSRIHKHVPAHLLLVGDGPDRSAAEWLALRKGIQDRVHFLGKQESVNELLPLAD
                     LMLMPSELESFGLAALEAMACRVPAIATKVGGVPELIEDGVNGRLFPVGHVEAMAEAA
                     IELLSSPEKLDTMATAARQTAQKRFCSTKIIPMYEDFYERVLERAGIAAGPVSPSS"
     gene            complement(3843..>4916)
                     /locus_tag="VFA02_08025"
     CDS             complement(3843..>4916)
                     /locus_tag="VFA02_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU09837.1"
                     /translation="GGAMLSLPAMLHHEYLSLPFNLAVGAIAGMYGKFVEKEEVWSFS
                     PFVDLSIYRWVRRNLRKPRFDRQVLLLALIVGLEISRGWLATMYPDRLFALVGPSWMI
                     RVAVWACSAMVVGIPLKIWNAIRIEQKLEEQKRLLLEARLDALQRQINPHFLFNTLNS
                     IASLVRFRPEQARELIVKLANILRALLREHDSYILLRDELSFTDDYLGIEVVRFGADK
                     LKVVKEIDPDTLDLPVPSMILQPLIENSIKHGLEPRISGGTITIRSKLDGGKLLIEVE
                     DDGVGIAPGRFHSSGVLRQGTGIGMNNVRERLEVLFGDVARFDVTSRPGRGTLVTMEM
                     PAANYEEGETGPAAMPARSNTRS"
BASE COUNT         1127 a         1398 c         1330 g         1061 t
ORIGIN      
        1 agatctgtgg ccaccttttc tgcatcttcg tcagaatgga ggtcccccag aaccacagta
       61 aattcttcgc ccccggtacg ggccaccgtg tctgattcac gcagccgttt cttcagacgg
      121 cctgctactt cctgaaggca gatatcgccc accgcgtggc cgtaggtgtc attgatttgt
      181 ttgaacctgt caagatcaat acacaaaatg gcggctttct gattgtggcg tgcagcgctt
      241 tccagggtgt gccggagccg ctgttccagg tggaagcggt tcggcaggtt tgtcagcgaa
      301 tcatggtttg cccggtgctc cagttgttca taaaggcgct gcttttccgt tacatcaata
      361 atcatgcaga accgcgcttt gcgcccggca aattctatga agtgagagga aatgtctgcg
      421 taaaacctgc tgccatcttt tctccggttc ttccacggac cggttgtcct cactgcctct
      481 cccggccggc tggacatgac cttttgtgct tcagagcggt ctgccatctc aatcagatca
      541 agtgcattga gggaaagaaa ttcttcgcgg gaatatccgt attgcgcaag ggccgcgtca
      601 ttgacgcgca ggaccctgga agaccctgga tcataaataa acatcgggtg cggattgctg
      661 tcgaacagca attgatactg ttcgcgctgc gtttcagcat ctacaatctg ttcctccagc
      721 aaagtaagaa tcatgccgaa ctcgacgaag tacttcggca cgttccagag atttggggaa
      781 atcacgagat gcggaaagaa atggtctgta aataagcctg ccggaaaaac cattgcccat
      841 gctacaagtc ccactgaggc cgtgatgacg ccgacggaaa cacgccggaa atcttcccgg
      901 aaaaggacgg cattcatgag aaatatctgc gtgaggacgg catttaatcc gagttcgggc
      961 cgtccgtggt ctatcagaat cagcagccac gtgccgcagg cgatggcaat caagttgttg
     1021 gcccagagga ccttctttct tttcctggca aagcgcgaac ctgcccaaat tacagcacat
     1081 tcgctggtat tgaccagaag aaccaggggc gcaacccgaa ggtaatcaaa agaaaccagg
     1141 aagatgtaaa taagcgcagg gatgctggaa atggccccga ttaagaagag atgccgcttt
     1201 gacaggcgcg tgactgtaga tgccagaaga aaacaggccc cggacaggac cagtccggct
     1261 gtactgattg cgccctgcac gcattgccag agataggtgc tgggatgggg ttccagtgca
     1321 gcaaaatgga acagcacaaa tatccagccg gctatccaga acagcagacg gggagacgtc
     1381 cgcttgcggt gaatcatgcc gaagagcagc accagcaggc ccaggatcag aatgtcttgc
     1441 caggtgtttt gcatcggtgc gctctctatt atttattcat cggcgaacaa cggaactgcg
     1501 tgcgaagaaa gtcgcaaaaa agcacagcaa gaacaggaaa aggaacgcac tgaccaatgc
     1561 acaagattct taaacgtccg tgcttattct ttatatgctt acttagatgg tgggcagtgc
     1621 agggctcgaa cctgcgacct cctgcttgta aggcaggcgc tctgaccaac tgagctaacc
     1681 gcccagggaa gataacccct tcctttcctg gggccagaca tcaaggcttt tttattctat
     1741 caggaacccg gcgtaaaagc tgctccaatg cgctgataaa ctggggcttg tgcgccgcct
     1801 gatcgtcaac gcagatgact ttggtcttac ttcgggaatt aaccgggccg ttaccgagtt
     1861 acactcggca cgcgcgttgt cctcggccac gctgatggcc actgcggaag cggccccatt
     1921 ctttcaggct gtaggagaaa ccagaaatca accctcgctt ggcgtcggct gccacatcgt
     1981 attggtggat ggcacccctt tgttacctcc atcgcaggtc ccttcgcttg tagacggcgc
     2041 tgaatttcgc ccgaagctcg gcgcattcgt caccgacctg ctgctcgggc gcatcgctga
     2101 agcagacatc gaagccgagg ccgtggcgca gatacgcaaa ttgcagcagg caggcgttcc
     2161 cgttacacat ctggacacgc acaaacacac acacaccttt ccacgcgtgc tgctgccttt
     2221 gctccgcgct gcacagcaat gcggcgtgaa agccattcgc aacccatttg aaccggattg
     2281 ggccttgaac gcgactgcca atgcgggcca tgtgcgcaag atgcaggtac ggctgctgcg
     2341 ttcgcaaagc cgtgcttttg cgcagcaggt caaccgcgca gggcttgtaa caacggacgg
     2401 cgccatcggc gtgctggcga ccgggacact cgatacacag acggtccaaa accttctggc
     2461 ggcgttgccg gaaggaacct gggagctggt ctgccatccg ggatacaatg atgccgacct
     2521 gaagaagcag cggacgcggc tccttgaatc ccgtgaagtg gaacggacgg cgctgcttga
     2581 aacggttcct gcggcccaag tggagctcgt tcattttggg caactgagtc cctgaagccg
     2641 tgtggatact ccgcacaagc gcagcattgt gaaaagacgt gagtgttttg aatccaatac
     2701 gaaagctcta ccgtccaatc cggtaatcgc atgaaaattg gaatcacctg ctatcccacg
     2761 tacggcggca gcggcgtagt cgccacggaa cttggcattg agcttgcccg cgctggccac
     2821 caggtgcatt tcattaccta ttcacagcct ttccggttga ccgggcgcga agaaggcatc
     2881 tgctaccacg aggtgccggt ctcgaattat ccgctgtttg agtatccgcc gtatgacctc
     2941 gcgctggcct cgcgcatggc cgaagtggca gagtattacg gccttgatct gcttcatgtg
     3001 cactatgcga ttccgcattc agtcagtgct ttgctggcgc ggcagatgct tgccacccgc
     3061 ggacgccacc tgccctttgt gacgacgctg catggcacag acattacgct tgtcggcctg
     3121 gaccgctctt atctgccgat cacgcagttt tccatcgaag aaagcgatgg gatcacttcc
     3181 atttcgcaat atctaaaaga ccgtacgatc aaggagttca agacaaaaaa tgaaattgag
     3241 gtcatcccca atttcgtgaa ctgcgatgtc tatgtgccat tacccgggga aatccggcag
     3301 gaagagcggg cgcgctatgc ggcccctggc gagaaacttc tgatccatct gtccaatttc
     3361 cgtccggtca agcgggtcac cgacgcaatc gaagtctttt cccgcataca caagcatgtg
     3421 cctgcgcatc ttctgctggt gggcgatgga ccagaccgct ccgccgctga atggctggcc
     3481 ttgcgcaagg gaatccagga ccgcgtgcat tttctgggta agcaggagag cgtgaatgaa
     3541 cttctccctc ttgccgatct catgctgatg cccagcgagc ttgagtcctt cggcctggca
     3601 gcactggaag ccatggcctg ccgtgttcct gcgattgcta caaaagtagg aggagtgccc
     3661 gaactcatcg aggatggtgt aaacggcaga ctctttccgg tgggccatgt cgaagcgatg
     3721 gcagaggccg caatcgagct gctctccagc ccggaaaagc tggacaccat ggccacagct
     3781 gcgcgccaga ctgcgcaaaa gcgcttctgc tctacaaaaa tcatcccgat gtacgaggac
     3841 ttttacgaac gcgtattgga gcgcgctggc attgccgccg gcccggtttc gccttcttcg
     3901 taatttgccg caggcatttc catcgtgacc agcgttccgc gccctggacg gctggtgacg
     3961 tcgaacctgg ccacgtcccc gaaaagcact tcgagccgct cccgcacatt gttcatgcca
     4021 atacctgttc cctgacgcag gactccgctg gagtggaaac gcccaggcgc gatgccaact
     4081 ccatcgtcct ctacttcgat cagcagtttt cctccatcga gcttgctgcg aatggtgatg
     4141 gttcccccgc tgatgcgcgg ctcgagtcca tgcttgatgc tgttttcgat caatggctgc
     4201 agaatcatgc tgggcacagg caggtcgagc gtgtcgggat caatttcctt cactaccttc
     4261 agcttgtccg ctccaaaacg cactacttca atgccgaggt aatcatcggt gaagctgagt
     4321 tcgtcgcgca gcaggatata agaatcatgc tctctgagca gcgcgcgcag gatgtttgcc
     4381 agcttcacaa tcaattcacg cgcttgctcc gggcgaaatc gcaccagcga agcaatcgag
     4441 ttcagggtat taaagagaaa gtggggatta atctggcgct gcaatgcgtc cagccgtgct
     4501 tccagcagca accgtttctg ctcttcgagt ttctgctcga tacgaatggc gttccatatc
     4561 ttaagcggga tacccaccac catggctgaa cacgcccaca ccgcgacccg tatcatccat
     4621 gaaggcccaa ccagtgcaaa aagacggtcg ggatacatgg ttgcgagcca gccgcgggag
     4681 atttccaatc caacgatcag cgccagcaac aatacctgac ggtcaaagcg cggcttacgc
     4741 aggttgcgcc gtacccagcg atagatgctg aggtccacaa acggcgagaa tgaccagact
     4801 tcttccttct caacaaactt tccatacatt ccggcaatcg cgcccacggc cagattgaac
     4861 ggaaggctca ggtattcatg gtgcagcatg gcgggaagcg aaagcatcgc gccccc
//
LOCUS       DASRBV010000213        15898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_621137, whole genome shotgun sequence.
ACCESSION   DASRBV010000213 DASRBV010000000
VERSION     DASRBV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15898
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621137"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFA02_08030"
     CDS             <1..266
                     /locus_tag="VFA02_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09838.1"
                     /translation="IPLLERATSINPKCAACFEKLGRALSATGKLQEGVEHLEKAVSL
                     DPKDAHLHYELGLALRKAGMNDRARAEMAIAQKQYGTKTADPR"
     gene            complement(387..1868)
                     /locus_tag="VFA02_08035"
     CDS             complement(387..1868)
                     /locus_tag="VFA02_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU09839.1"
                     /translation="MRLRPPVRISSGALHTMLATAFLCLSGCTVGPSYKRPSTLTPPP
                     PAFKEAVPPPGIPNGGWKQAQPNDQSVRGKWWEIYNDPQLNTLEEKVSVSNQTLRAAM
                     EQYFAARSAVQTYRANYFPVLSAGPSISRQHLSQNRPTAVPGSRYQYNDFALTGQASW
                     EPDLWGQVRRSVEAARATAQASAADLANVELSIRSELALDYFELRGLDLQKQLLDNTV
                     ASYEDYLKLTQVRFKGGLATDSDVALAETQLESTRSQDIDVGVARAQYEHAIATLTGQ
                     TASAFSLPPAPLNLPLPQVPLIVPSELLERRPDIAGAERRADAANAQIGIAISAYYPT
                     ITLSGAGGFESRSPGTWIQGPSALWSLGASASELLFDAGRRHALTEEARDNYEVTVAN
                     YRQSVLNAFQEVEDNLAALRILQQEAVTQAAAVAAAQRSLDISTRRYRQGLVDYLEVL
                     TAQSALLTNQRTQADITTRQFAASAQLIKALGGGWDTSQLPNP"
     gene            complement(1871..3064)
                     /locus_tag="VFA02_08040"
     CDS             complement(1871..3064)
                     /locus_tag="VFA02_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09840.1"
                     /translation="MSNSTQAQQTPRPVSRGTMIVLLIVFLIAAVVAAIAGILPRLHD
                     RARLQEETNQLAAPDVQVATPQQGAPAEEVVLPGNIFAYVDSPIYARTSGYLKKWYFD
                     IGAHVKKGQLLAEIESPEVDQQLAQAKADLLTAEANSKYAQATASRYQDLVKSNAVSR
                     QDTENFTTQASASSTQVKSALANVQRLEQLVSFEKVYAPFDGVVTARGIDIGQLIDAG
                     ANRELFHLSDEHILRVYVNVPQVYSLASRPGLTANLTFVEYPGRTFQGKIVRTAKAID
                     PTSRTLLVEVDVDNRKGELVPGAYAQVHFKLQNPVRSLIVPVPALLFRSEGLRVAIVE
                     NNKIKLVPITIGRDDGRVVEVTSGLQPTDQVVQNPPDSIIDGEVVHVVQPGQQNPQAR
                     QNGGE"
     gene            complement(3057..6344)
                     /locus_tag="VFA02_08045"
     CDS             complement(3057..6344)
                     /locus_tag="VFA02_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU09841.1"
                     /translation="MWIVRLALRRPYTFAVFALLLMILGPVAILRTPTDIFPNIDIPV
                     VAIAWSYTGLSPEQMARRIVYQSERSLTTTVNDIEHIESQSLNGMGIIKIFFQPKVNI
                     AMAVAQVTAIAQTQLRQLPQGTQPPLILQYNASSVPILQLGLSGEGLSEQQLNDYALN
                     FIRTQLVTVNGVGIPYPYGGKQRQIQVDLNLQALQSKGLSPADVTSTIANQNLILPSG
                     TAKIAQFEYQIETNSAPETVQELNNLPIRAVNGAMVYIRDVAHVRDGFPPQTNIVRVD
                     GQRASLLSIIKTGDASTLDIVKGVRDKLPAIKAQLPPQLRIIPLADQSIFVRAAITGV
                     VREAIIAACLTAIMILVFLGSWRSTLIIAVSIPLSILTSIIVLSALGETINIMTLGGL
                     ALAVGILVDDATVEIENINRNLEQGKAIEQAILDGAAQIAVPAFVATISICIVFVPMF
                     FLSGVARYLFVPLAEAVVFAMLASYLLSRTVVPTMAKYLLREHSDEEVERRQQSRNPL
                     IQFQLAFEHGFERVRRFYYGILQMCIAHSAVFLVLFIVFCLGSAALLFPFVGEDFFPS
                     VDAGQFKMHLRAHTGTRIEETARLCDEVDSTIREVIPKNELVTIIDNIGQPYSSINMT
                     YSNSAIIGPADADIQVSLTEHHHPTEAYVQKLRSVLAARFPGVTFYELPTDMVTQILN
                     FGLPAPIDIQVTGPDLQGNRAFAEQLLNRVKYVPGTTDMRIQQPFDNPNLFIDVDRTK
                     AGQMGLTQGNVAQSLLVSTSGSFQTSPNFWLNPRTGVSYSIAVQSPQYDLDTLQDIEN
                     IPVTPSGSNATNAATAASHGAGGPVLPANTPMGGQPVQILGNLASIAPGSEMGTESHY
                     NIAPVIDIYGNVENSDLASVNKQIEQIIGQMKSKLPRGSQIVVRGQVETMRTSFIGLL
                     GGLVFSVVLVYLLIVVNFQSWIDPFIVISALPAALCGIIWFLFLTHTRISVPALTGAI
                     MCMGVATSNSILVISFARERLEENVGDALEAALSAGFTRFRPVLMTALAMIIGMAPMA
                     LGLGEGGEQNAPLGRAVIGGLLFATVSTLFFVPTFFSVIHGWLGRRRRHKRQDALGET
                     AEGKGHE"
     gene            complement(6664..8118)
                     /locus_tag="VFA02_08050"
     CDS             complement(6664..8118)
                     /locus_tag="VFA02_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZU09842.1"
                     /translation="MSTQAQSQALPNSRPLCVDLDGTFIKSDTLADSFFVLLRMRPLT
                     ALKAPFWLPGGKAALKAKISSIVSLDAAHLPYNRPLLNFLAEEHSRGRRIFLTTGADG
                     KLAEEIAAHVGVFDGVIASDGRTNLTGNNKLASLHAEFAESGFDYIGNALPDLPVLQH
                     ATEAMLANPRSSLLSRVRSRGIPIVRTFNDQTAWTKALLRAIRPHQWAKNVLIFVPLL
                     LAHTLRPPLVLNVLLAFICFSLCASASYIINDMLDMEADRRHPAKRYRPFAAGDLQAT
                     TGAAISLVFLAMAFTGAALMLPPQFSGWLALYLITTLAYSLVLKKKALVDVVLLSGLY
                     TLRLLAGGAAAETSVSAWLAAFSAFFFFSLALVKRFSELENLRAQGRLFASGRGYRVS
                     DAAQLRSFGTSSAFAAVVVFALYINGDKATALYRHPERMWLIVPLLLYWISRIWLLAS
                     RQELNEDPVIFAVTDRPSLIIGFAVVVIAIFAAL"
     gene            complement(8147..8278)
                     /locus_tag="VFA02_08055"
     CDS             complement(8147..8278)
                     /locus_tag="VFA02_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09843.1"
                     /translation="MGSASLAPIYTLYAKPCINVTPEEAGQPSAPPSFSLAKPAKVD"
     gene            8413..9672
                     /locus_tag="VFA02_08060"
     CDS             8413..9672
                     /locus_tag="VFA02_08060"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HZU09844.1"
                     /translation="MSSTQTTQPATGTAPVVPPPQRHRIRTTIIFLLVVGVVGFIIWR
                     IVTANDKANKQAAAQQAAMANRAIPVQVATVQEKAMPIYVTALGTVSPYYSVTVKPRV
                     TGELIRVNFKEGQDVHQGEELMTIDPRPYQATLDQAKGQLAKDQAQLQNAQAEYDRYK
                     ALFDQGVVSKETLDAQAANFGNYEGAIKADQAAIDADALNVQYCHITSPITGKIGLRL
                     VDPGNVVTANTTNLIVINQFQPIAVLFTLPEDQLPRVFAKMSGARALQAEAYDRSDTI
                     HIASGQLLTADNQIDTTTGTGKLKAVFPNKDEQLFPNQFVNVHLVLETRPDAITAPTA
                     AVQHGTDGDFVWIVKPDKTVAVQPVKVDVTEGTTVIFSDGLKPGDQVVTDGADKLREG
                     TKVDARPMTHNRPATQSGNGSNGMMGQ"
     gene            9679..12792
                     /locus_tag="VFA02_08065"
     CDS             9679..12792
                     /locus_tag="VFA02_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HZU09845.1"
                     /translation="MSPSRPFILRPVATSLLMAAILLAGFVAYRQLPVSALPEVDYPT
                     IQVVTFYPGASPDVMASSVTAPLERQFGQMPGLNQMTSTSSEGGSVITLQFTLSEDID
                     VAEQEVQAGINAAQSFLPADLPNPPVYSKVNPADQPILTLALTSKTMPLPQVEDLADT
                     NLAQKISQVSGVGLVSIAGGQKPAVRIQANPSQLAAYGLSLEDIRTQVAANNINQAKG
                     SLNGTRQSFTIDANDQLLNSTSYNPIVVAYRNGAPVRLQDVASVVNGAENSLRASWMN
                     EVPAVIVNIQRQPGANIIGVVDRIEKILPQLKASLPASIDVVTLTDRTVTIRASVKDV
                     QFELMLTIALVVMVIFLFLRNLSATIIPSVAVPLSLVGTFGVMYLLGYSLNNLTLMAL
                     TISTGFVVDDAIVMIENISRFLEEGDSPLEAALKGSEQIGFTIVSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAITLAVTILVSAVVSLTLTPMMCAKILRHKPPEKQGRFYRWTEDL
                     FDRIIGFYGRTLQTVLRFRFTTLMVTVATLVFTVLLYIYVPKGFFPVEDTGVLLGISE
                     APQTVSFREMARRQEALAHVILQDPDVESLSSFIGVDGTNMTMNTGRIQINLKPLDQR
                     KSRAQDIIRRLQPKLAQVEGITLYLQALQDLTVADTVSRTQYQYTLEDVDPRELGTWV
                     PRIVAELQKQPELRDVASDLQDNGLQTTLDIDRDTASRLGVSMQNIDNVLYDAFGQRQ
                     VSTMFTQLNQYHVVLEVQPEFQRTADDLSKIYVPSSTGTQVPLSAFVKISQTNTPLAI
                     NHLGQFPVATISFNLAPGASLGDAVQAIHNVEQRMGMPASINAAFQGTAAAFQASLSN
                     EPLLILAALITVYIVLGVLYESYIHPITILSTLPSAGVGAILALLLFKLDLNVIGIIG
                     IILLIGIVKKNAIMMIDFALEAERKEGKSPEESIYEACLLRFRPIMMTTMAALLGGLP
                     LAFGSGMGAELRQPLGITIVGGLIFSQVLTLYTTPVVYLYFDRLGQWFMRRRERRSLE
                     EQAVHAD"
     gene            12804..>15898
                     /locus_tag="VFA02_08070"
     CDS             12804..>15898
                     /locus_tag="VFA02_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZU09846.1"
                     /translation="MHLSAPFIRRPVATTLLSLALLLAGAVAYNLLPVASLPEIEFPT
                     ISVYGGLPGASPETVAAAIATPLERQFGRIAGVTQMTSSSSLGGSNVNLQFDLSRDIN
                     GAARDVQAAINAARSNLPANMPENPTYRKINPSDAPIMIIALTSDVMTQGQMYDAADS
                     ILAQKISQIQGVGQVFVGGSAQPAVRVEVNPMQLSSYGIGLEGVRTALSQVNMNLAKG
                     FLKAGGRMSSIADNDQLMKASQYAPLVIGYNPATGAAVRLSDISQVVDSVSDVHTAGL
                     ANGKRAVLVIVFKVPGANVISTVDRIYAAIPALKASISPLIDFHVVLDRTITIRASVL
                     DVEISLLISVTLVVLVVFLFLRNVWATIIPSVAVPLSLVGTFGVMYLLGYSLDNLSLM
                     ALTISTGFVVDDAIVVIENITRYLEMGLSPYEAAVKGSKEIGFTVLSMSTSLIAVFIP
                     ILLMGGVVGRLFREFAVTLSVAIAMSLVVSLTTTPMMCAKFLKPHNQQKHGRIYMLSE
                     RFFTGMLKMYDHGLRWVIAHKVPVLLITIATFFLNIYLFILVPKGFFPQQDTGRMMGI
                     TNASQDISFEAMQQKQAQLAAIVQADPGVQSVMAFAGGSGGGATTNVGRMFITLKPLD
                     ERHGVSADQIINRLRPKLAHIPGVTLVLQAAQDLSVGARMSAAQYQYTLEDQDIKELN
                     EWAPRLLEKMRTMPELRDASTDQQDQGLAANLVIDRDTAARLGISPSTIDNVLYDAFG
                     QRQVSTMYTSLNQYHVVMEVAQPYQRDPQALSNIYIKSSNGTSVPLTAVTKFEPGRTS
                     LAVNHQGQFPAVTLSFNLAPGVSLGQAVVALQNAEADMGMPSTVHGSFQGTAQAFQSS
                     LSSEPFLILTALATVYIVLGILYESYVHPITILSTLPSAGVGAILALLITHTDLSVIA
                     IIGIILLIGIVKKNAIMMIDFALAAERNEGKSSDEAIYEACLLRFRPIMMTTMAALLG
                     GLPLALGRGTGSELRQPLGITIVGGLIVSQMMTLFTTPVVYLFFDKLQLRVQRFGKRF
                     E"
BASE COUNT         3439 a         4708 c         4411 g         3340 t
ORIGIN      
        1 ccattccact gctggaacgt gcaacgagta tcaatccaaa gtgcgctgcg tgtttcgaaa
       61 agctgggccg cgcgctcagc gcaacaggaa aattgcagga aggcgtggag catctggaaa
      121 aggccgtctc ccttgatcca aaggacgccc atctgcacta tgagctggga ttggcgcttc
      181 gcaaggccgg catgaatgat cgcgcccgcg ccgagatggc tatcgcccag aaacaatacg
      241 gtacaaaaac tgccgaccca cgctaacggt aagcaccgga ctgtggccgt tgaatcagca
      301 tgagagaatc agggacaagc cccactccgg cattttcata gatagtttgc accatgccgc
      361 gaaactacac tcgccgcggc ctgacgctat ggattcggta actgcgaggt gtcccatcct
      421 ccacccagcg ccttgatcaa ctgcgcgctg gcggcgaatt gccgcgtggt gatgtctgcc
      481 tgcgtgcgct ggttcgtcag cagcgcgctt tgcgcagtaa ggacttcgag gtaatccacc
      541 aggccctgcc tgtaccgccg ggtactgatg tccagcgagc gctgcgccgc ggccacggct
      601 gcggcctggg taacggcttc ctgttgaaga atgcgcaatg cggcgaggtt gtcctccact
      661 tcctgaaatg cattcaaaac actttggcga tagttggcga cggtgacttc ataattgtcg
      721 cgtgcctctt ctgtgagagc gtggcggcgt cctgcatcaa aaagaagctc ggaagcagaa
      781 gcgcccagag accacagcgc gctgggcccc tggatccatg tgccggggct gcggctttca
      841 aatcctcccg caccggaaag cgtaatggtg gggtaatacg ccgaaatggc gatgccgatc
      901 tgcgcgttgg cggcatcggc gcggcgttct gccccggcaa tatcaggacg gcgctccagc
      961 agctctgagg ggacaatgag aggcacctgc ggcagaggca gattcagcgg cgctggcggc
     1021 aggctgaaag ctgacgccgt ctggccggtc agcgtggcaa tggcgtgttc atattgtgcg
     1081 cgggccacgc ccacgtcaat gtcctgtgag cgcgtgcttt caagttgtgt ctctgccaga
     1141 gcaacgtcgg agtcggtagc gagaccgcct ttgaagcgca cctgcgtgag tttcaggtag
     1201 tcctcatagg aggccacagt gttgtcgagc aactgtttct gcaggtcgag tccgcgcagt
     1261 tcaaagtagt ctagtgccag ttcagagcgg atgcttaatt ccacattggc cagatcggct
     1321 gcgctcgcct gtgcagtggc gcgagcagct tccacgctgc gccgcacctg tccccacaga
     1381 tcaggctccc aactggcctg cccggtcagg gcgaagtcgt tgtattgata tcgcgagccg
     1441 ggaacagcag tcgggcggtt ctgggaaaga tgctggcgcg aaatggaggg tcctgcgctg
     1501 agcacaggga agtaatttgc gcgatatgtc tgcaccgcag accgtgctgc gaagtattgc
     1561 tccattgctg cccgcagcgt ctggtttgag acagagactt tttcttcaag cgtattcagt
     1621 tgcgggtcgt tgtagatctc ccaccacttg ccgcgcacgg actggtcatt cggctgcgcc
     1681 tgcttccatc ctccgttggg gatgcccgga ggaggaacgg cctccttaaa tgccggaggg
     1741 ggcggtgtga gtgtggaagg gcgcttgtaa ctggggccga cggtacatcc ggaaagacag
     1801 agaaatgccg tggcgagcat ggtgtgcagg gcacctgaag atatgcgaac aggcgggcgc
     1861 agacgcattc ttactcgcct ccgttttgcc gcgcctgcgg attctgctga ccgggctgta
     1921 ccacatgcac cacttcgcca tcaatgatgg agtccggcgg attctgcacc acctggtccg
     1981 tgggctgcaa accagaagtt acttccacca cgcgtccatc atcgcggcca atggtgatgg
     2041 gcaccagctt tattttattg ttttcgacaa tggcgacacg cagcccttcg gagcggaaca
     2101 acagcgcagg gacaggaaca atcagcgacc gcaccgggtt ttgcagcttg aagtggacct
     2161 gagcataggc gccaggaaca agttctccct tgcgattgtc tacatctact tcgaccagca
     2221 atgtccgtga tgtgggatca atggccttcg ccgtgcggac gatttttccc tggaacgtgc
     2281 gcccgggata ttccacaaaa gtaaggtttg ccgtaaggcc cggcctggag gcaagcgagt
     2341 agacctgcgg cacattcaca tagacgcgca gaatatgttc gtcagagaga tgaaacagct
     2401 cgcggttggc tcctgcatca atgagttgac caatgtctat accgcgggcg gtgactacgc
     2461 catcaaaggg cgcatacact ttttcaaacg agaccagctg ctccagacgc tgcacgttgg
     2521 ccagcgctga tttgacctgt gtgctcgaag ctgatgcctg cgtagtgaag ttttccgtgt
     2581 cctggcggga gaccgcattg ctcttgacca ggtcctgata tcgtgaggcc gtggcctgcg
     2641 catattttga attggcttcc gcagtgagca gatcagcttt ggcctgcgcc aactgctggt
     2701 ctacttcagg gctttcaatc tccgccagta actggccctt ttttacatgc gcgccgatgt
     2761 cgaaatacca ctttttaaga tagccgctgg tgcgcgcata aatgggcgaa tccacatagg
     2821 caaaaatgtt cccgggcagc acgacttctt ctgctggagc gccttgttgt ggagtggcca
     2881 cctgcacgtc aggagcagca agctggttcg tctcctcctg caatctggcg cggtcatgca
     2941 gacgcggtag aatgccggca atggccgcaa ctaccgcggc aatcagaaac acaatcagaa
     3001 ggacgatcat cgtcccgcgc gaaaccggtc gtggtgtctg ctgcgcttgt gttgaattac
     3061 tcatggccct ttccttccgc tgtttctccc aatgcgtcct gccgtttgtg cctgcgcctg
     3121 cgtcccagcc atccgtggat cacgctgaag aatgttggca cgaaaaacaa agtggagacg
     3181 gtagcaaaca acaggccgcc aattacggcc cgccctaaag gagcattctg ttcgccgcct
     3241 tcgcccagtc ccagcgccat cggcgccata ccgataatca tggcgagtgc cgtcatcaac
     3301 accggacgaa aacgcgtaaa tcccgcagac agggccgctt caagcgcgtc gccgacgttt
     3361 tcctccagac gctcgcgcgc gaagctgatg acgaggattg agttcgatgt cgccacgccc
     3421 atgcacataa tggcccccgt cagcgcgggc acgctgattc gcgtatgcgt aagaaacagg
     3481 aaccagataa tgccgcacaa cgccgcaggc agtgctgaga tgacgatgaa cgggtcaatc
     3541 caggactgaa agttgaccac aatcagcaga taaaccagca caaccgaaaa caccagcccg
     3601 cccagcagac caatgaagga cgtgcgcatc gtctccacct gaccgcgtac gacaatctgc
     3661 gaaccacgcg gcagtttgct cttcatctgc ccaatgatct gctcaatctg cttgttcacg
     3721 ctggccaggt cactgttctc aacgttccca tagatatcaa tcacgggcgc aatgttgtag
     3781 tgggattccg tgcccatctc agagccgggc gcaatcgaag cgagattgcc cagaatctgc
     3841 accggctgac cgcccatggg ggtgttggca ggaagcacag gcccgcccgc gccatgactg
     3901 gccgctgtgg ctgcatttgt ggcgttggaa ccgctcggcg tcacaggaat gttctcgatg
     3961 tcctgcagcg tgtcgaggtc atattgtggc gactgcacag cgatgctgta gctgacgccc
     4021 gtcctcgggt tcagccagaa attcggcgat gtctgaaagc tgccgctggt ggaaacaagc
     4081 aggctttgcg ccacattgcc ctgggtcagt cccatctggc cggctttggt gcggtccaca
     4141 tcaataaaga gattgggatt atcaaagggc tgctggatgc gcatgtctgt cgttccggga
     4201 acatacttca cgcggttcag cagttgttcg gcaaaggcgc gattgccttg caggtccgga
     4261 ccggtcacct ggatatcaat cggcgcaggc agaccaaaat tcaggatctg cgtcaccatg
     4321 tcggtgggca gctcgtagaa cgtcactccg ggaaaacgcg ccgccagcac cgaacgcagc
     4381 ttctgcacgt acgcctctgt cgggtggtgg tgctccgtca gggacacctg aatgtccgcg
     4441 tccgccggtc caatgatcgc cgaattgctg tacgtcatgt tgatgctgct gtagggctgt
     4501 ccaatgttgt caataatcgt cacaagctcg ttctttggaa tcacctcgcg gatcgtgctg
     4561 tccacttcat cgcagagccg cgccgtctcc tcgatgcgcg ttcctgtgtg ggcgcgcagg
     4621 tgcatcttga attgccccgc gtcaacagac gggaaaaagt cttctcccac gaacgggaaa
     4681 agcagtgcag cagatcccag acaaaagacg atgaacagca ccaggaacac cgcagaatgc
     4741 gctatgcaca tctgcagaat tccgtagtag aagcggcgta ctctctcaaa cccgtgctca
     4801 aaggcaagct ggaactggat gagcggattg cggctctgct gcctccgctc cacttcctcg
     4861 tcgctgtgct cgcgcagcag atactttgcc atcgttggca caaccgtgcg cgagagcaga
     4921 taagaagcca gcatcgcgaa cacgactgcc tctgctaatg gcacaaacag ataccgcgcc
     4981 acacccgaaa ggaagaacat cggcacaaag acaatgcaga tagagatggt ggcaacaaac
     5041 gccggaacgg caatctgcgc cgcgccatcc agaatcgcct gctcaatggc cttgccctgt
     5101 tctagattgc ggttgatgtt ctcgatttcc accgttgcat cgtccacaag aatgccgacc
     5161 gcaagcgcca gtccacccag ggtcatgatg ttaatcgttt caccgagggc gcttaaaaca
     5221 atgatcgaag taagaatcga gagcgggatc gagacggcaa taatgagtgt gctgcgccag
     5281 ctccccagaa acaccagaat catgattgcc gtcagacagg ccgcaatgat cgcctcacgt
     5341 accacgcccg tgatcgcagc ccgcacaaaa atggactggt cagccaaagg aatgattcgc
     5401 agctgcggcg gcaactgcgc tttaatcgcc ggaagcttgt cccggacacc ttttacgatg
     5461 tccagcgtag acgcatcgcc cgttttgatg atgcttagaa gcgaagcgcg ctgcccgtcc
     5521 acgcgcacaa tgttggtctg cggaggaaag ccatcgcgca catgcgccac gtcgcggata
     5581 tacaccatgg cgccattaac ggcgcgaatc ggcaggttat tcagctcctg gacggtttcc
     5641 ggagcgctgt tcgtctctat ctgatactca aactgcgcga tttttgccgt gcccgaaggc
     5701 agaatcaggt tctggttggc aatcgtgctg gtcacgtccg ccggcgaaag ccctttggac
     5761 tgcaatgcct gcagattcag gtctacctgg atctgccgct gctttcctcc atacggatag
     5821 ggaatgccaa ctccatttac cgtcacaagc tgggtgcgga taaagttcag tgcgtagtcg
     5881 ttgagctgtt gttctgaaag tccttcgccc gacaagccaa gctgcagaat cggaacgctg
     5941 gatgcgttgt attgcagaat aagcggcggc tgggtcccct gcggcaactg gcgcaactgc
     6001 gtctgtgcaa tggccgtgac ctgtgccaca gccatggcga tgtttacttt gggttgaaag
     6061 aaaatcttga tgatgcccat gccattgagc gactgcgact caatgtgctc gatgtcgttg
     6121 acggtcgttg taagcgagcg ctcactctgg tacacgatgc gccgggccat ttgctccgga
     6181 gacagccccg tatagctcca cgcaatcgcc acaacaggga tatcaatgtt aggaaagatg
     6241 tctgtgggcg tgcgcagaat ggccaccgga ccaaggatca tcaggagcag ggcaaaaacg
     6301 gcaaacgtat aaggccgtcg cagcgcgagg cgaacaatcc acatggctcg tcaatctctc
     6361 ctgcggccag cgcgaatgcg cgaccagaaa agggtggtct tttgattaca cgttagaaga
     6421 aagcagacag aaccccgttt tcctcgtgaa acctgccgga acaatatgtc attgtgttgg
     6481 cctgatcttg gccagacaat cgaacaaact tctattttaa agtagacggt catgacgcca
     6541 aaaaggccga agccctcaac ctcatatgca agcaagcaat ctcagaaatt acgtggatag
     6601 aggccccggc cctctcggta cctgttctga ggtgttccgg aacggaaatt gatgccgggc
     6661 gcgttacaaa gcagcaaaaa tggcgattac aaccacggca aaaccgataa tcaggctcgg
     6721 ccggtcagtg accgcaaaga tcacagggtc ctcgttcagt tcctgtcgcg aagccagaag
     6781 ccagatgcgg ctgatccagt acaacagcag gggaacgatg agccacatgc gctccggatg
     6841 gcggtaaagc gcagtggcct tgtctccgtt gatatagagc gcaaacacaa ccactgcggc
     6901 aaaggcgctg gaagtaccga agctgcgcaa ttgggccgca tcggaaacac gatacccgcg
     6961 cccggaggcg aaaaggcgcc cctgcgcgcg caggttttcc agttcgctga atcgcttcac
     7021 cagggcaagg gagaaaaaga agaaagcaga aaatgcggcc agccacgcgg acactgaagt
     7081 ctccgcggcc gctccgcctg caagcagccg cagcgtgtag aggcccgaaa ggagaacaac
     7141 gtctaccagt gcctttttct tcaggaccag cgaataggcc agggttgtta tcagatagag
     7201 cgccagccat ccgctgaatt ggggcggaag catcagggcc gcgccggtaa aggccatcgc
     7261 aagaaagacc aggctgatcg ctgctccggt ggttgcctgt aaatctccgg cagcaaatgg
     7321 acggtatcgt tttgccgggt gccgccggtc agcttccatg tccagcatgt cattgatgat
     7381 gtagctggca gaagcgcaaa ggctgaagca tatgaaagcg agcagcacat tcagtacaag
     7441 gggtggccgc agcgtgtgcg ccagaagcag cggcacaaaa atcagaacat tcttggccca
     7501 ttgatgcggc cgtatggcgc gcagaagtgc ttttgtccag gcagtctggt cattgaaggt
     7561 gcggacgatg ggaatgccgc gtgagcgcac tctgctcagg agcgaggagc gagggttggc
     7621 cagcatggcc tcggttgcat gctgcaaaac gggcaggtcg ggaagcgcgt ttccaatgta
     7681 atcaaagccg ctttctgcga attcagcatg cagggacgca agtttgttgt tcccggtgag
     7741 attggtgcgg ccatcgctgg caatcacgcc atcaaagacc cccacatgtg cggcgatctc
     7801 ttcggccagt ttgccgtcgg ccccggtagt gagaaatatc ctgcggccgc ggctgtgctc
     7861 ttctgcaaga aaattcaaca gaggccggtt gtaaggcagg tgcgctgcat ccagcgaaac
     7921 aatgctgctg atttttgcct tcagcgccgc ttttccacca ggaagccaga aaggcgcctt
     7981 cagcgcggtc agaggacgca tccgaagaag cacaaaaaac gagtcagcca gtgtgtcgct
     8041 cttgataaat gtgccatcca gatctacgca caaggggcga gaatttggaa gggcctggga
     8101 ttgcgcctgc gtggacagga aatctctccg gtattggact ctcctatcag tctactttgg
     8161 ccggcttcgc cagagaaaag ctaggtggcg cgctgggctg acctgcttct tccggcgtga
     8221 cgttgatgca gggctttgca tacaatgtat aaattggtgc aagagaggcg gatcccattc
     8281 cgatctcgca gattttcgag ggatttccgg gaaacatttc cggacgctgg ttcgtcggta
     8341 taaatactgc ttccgctcca tgttaggggc cggcaattta aatcaaaacc ggggaagtca
     8401 tcggaagaat ttatgtcgtc cactcaaacc acgcagcctg ccaccgggac tgcgcccgtt
     8461 gttcctcctc cgcagcgcca tcgcatccgc actaccatca ttttcctgct ggtagttggc
     8521 gtggttggat tcatcatctg gcgcattgtt accgcaaatg ataaggccaa taagcaggct
     8581 gcggcgcagc aggcggccat ggccaaccgc gccattcctg tccaggtagc aaccgtgcag
     8641 gaaaaggcga tgcctattta tgtgaccgcg ctgggcacag tcagtccata ttattcagtc
     8701 acagtcaaac cgcgggtcac aggcgaattg atacgcgtga atttcaaaga agggcaggac
     8761 gtgcatcagg gcgaagagct gatgacgatt gatcctcgtc cctaccaggc cacgctcgac
     8821 caggccaaag gccagcttgc caaagaccag gcacagttac aaaatgccca ggctgaatat
     8881 gaccgctata aagcgctctt cgatcagggc gtcgtctcca aagaaacgct ggacgcgcag
     8941 gcagcgaatt tcggcaatta cgaaggcgcc atcaaggccg accaggcggc gattgacgcc
     9001 gatgcgctca atgttcagta ctgtcacatc acgtcgccca taaccggaaa aatcgggctg
     9061 cgccttgtag accctggaaa cgtggttact gcaaacacga ctaacctgat cgtcattaac
     9121 caattccagc ccatcgccgt tctctttact cttcctgaag accagttgcc gcgcgtgttt
     9181 gcgaaaatga gcggagccag ggcgctccag gccgaggcgt atgaccgctc tgacaccatc
     9241 catatcgcca gcggacaatt actgaccgct gacaaccaga ttgacaccac caccggcacg
     9301 ggcaagctga aggcggtttt cccaaataaa gacgagcagt tgtttcccaa ccagtttgtg
     9361 aatgtccatc tggtgctcga aacacggcct gacgccatta ccgctccgac tgccgccgtg
     9421 cagcatggca cagatggcga cttcgtctgg atcgtaaagc ctgacaagac cgttgccgtt
     9481 cagccggtaa aagtagacgt gaccgaaggc acgacggtca tcttttcgga cggcctgaaa
     9541 cctggcgacc aggtagtgac cgacggcgca gacaagctgc gtgaaggcac aaaagtagat
     9601 gcgcggccca tgacccacaa ccgtccggca acgcagagcg gaaatggcag caatggaatg
     9661 atggggcaat aggcggtcat gagtccttcc cggccattta ttctccggcc agtagcgaca
     9721 tcgctgctta tggcggcgat tctgcttgcc ggctttgtgg cgtaccgcca gcttcctgtc
     9781 tctgctctgc cggaggtgga ttaccccacc attcaggtag tcacgtttta tcccggagcc
     9841 agcccggacg tcatggcctc ctccgtgacg gccccgttgg aacggcagtt cggtcagatg
     9901 cccggcctca accagatgac ttccaccagc tctgagggtg gctcggtcat cacactgcaa
     9961 tttacgcttt ctgaagatat tgacgttgcc gaacaggagg tgcaggccgg aatcaatgct
    10021 gcgcaaagct ttttgccggc cgacctgccg aatcctcccg tctacagcaa ggtgaacccg
    10081 gcagaccagc ccattctcac actggccctg acttcaaaga ccatgccttt gccgcaggtc
    10141 gaagacctgg ccgacacgaa cctcgcgcaa aaaatatcgc aggtttccgg cgttggcctg
    10201 gtctccattg ccggtggaca gaaaccggct gttcgcattc aggccaatcc atcccagctt
    10261 gcggcctatg gtctcagcct cgaagatatc cgcactcagg tggctgccaa caacatcaat
    10321 caggcaaaag gaagcctcaa cggcacacgc cagagcttta ccattgatgc aaacgaccag
    10381 ctcctgaact caacttcgta caatcccatc gttgttgcct atcgcaatgg cgcgcccgtg
    10441 cgtttgcagg acgtcgcttc agtagtcaat ggcgcagaga actcgctgcg cgcttcatgg
    10501 atgaatgaag tccccgctgt gatcgtaaat atccagaggc agccgggggc caacatcatc
    10561 ggggtcgtag accgcatcga gaagattctg cctcagctca aggcaagcct gcctgcttcc
    10621 attgatgttg tcacgctgac cgaccgcacg gtgaccatcc gcgcctccgt aaaagacgtg
    10681 cagtttgagc tgatgctgac cattgccctc gtcgtgatgg tcatcttcct cttcttacgg
    10741 aatctctccg cgaccatcat tcccagcgtt gccgtgccgc tttcgcttgt tggcaccttc
    10801 ggcgtgatgt atctgctggg ctacagcctg aacaacctta ccctgatggc gctgaccatc
    10861 tcgactggat tcgtggtaga cgacgccatc gtgatgattg aaaacatcag ccggtttctg
    10921 gaagaaggcg attcgccgct cgaggccgcc ctcaaaggct ctgagcagat cggttttacc
    10981 atcgtctcgc tgaccgtctc gctcattgcc gtgctcattc cgctgctctt tatgggcgac
    11041 gtggtgggcc gcctcttccg cgaattcgcc attaccctgg ccgtcaccat tctggtttcc
    11101 gcagtggttt cgctcactct cacgccgatg atgtgcgcga agatactccg ccacaagccc
    11161 ccggaaaagc agggccgctt ttaccgctgg acggaagacc tcttcgaccg catcattggc
    11221 ttttatggcc gcacactcca gaccgttctg cgcttccgct tcaccacgct gatggtcaca
    11281 gtggccacgc tcgtctttac cgtgttgttg tacatctatg tgcccaaagg cttctttccc
    11341 gtagaagaca caggtgtgtt gctgggcatc tcagaggcgc cgcagacagt ttccttccgg
    11401 gaaatggccc gccgccagga agcactggca cacgtcattc tgcaggaccc ggatgtagaa
    11461 agcctctcgt cctttatcgg tgtagatggc accaacatga cgatgaatac cgggcgcatc
    11521 cagatcaatc tgaagccgct tgatcagcgc aaaagccgcg cccaggacat cattcgccgc
    11581 ctgcagccca agctggcgca ggtggaaggc atcacccttt atctgcaggc cctgcaggac
    11641 ctgaccgtgg ccgataccgt gagccgcacg cagtaccagt acacgctcga agacgtggac
    11701 ccgcgcgagc tgggcacctg ggtgccgcgc atcgtcgccg agctgcaaaa gcaacctgag
    11761 ctgcgcgatg tcgccagcga tctgcaggac aatggtcttc agaccacgct cgacattgac
    11821 cgtgacaccg cctcgcgcct cggcgtttcc atgcagaaca ttgataacgt gctgtatgac
    11881 gctttcggtc agcggcaggt ctccaccatg ttcacgcagc tcaaccagta ccacgtggtg
    11941 ctcgaggtgc agcccgaatt tcagcgcacc gccgatgacc tgagcaaaat ctacgttccg
    12001 tcatccaccg gaacacaggt gccgctcagc gccttcgtaa aaatctcgca gaccaacacg
    12061 ccgctggcca tcaatcacct cggccagttc cccgtcgcta ccatttcatt caatcttgct
    12121 cccggagcat cgctgggcga cgcggtccag gccatccata atgtcgagca gcgcatgggc
    12181 atgcccgcca gcatcaacgc tgcttttcag gggaccgccg cggccttcca ggcgtcgctc
    12241 tcaaatgaac ctctgctgat tctcgctgcg ctcataaccg tatacatcgt gctcggcgta
    12301 ctctacgaga gttacatcca tcccatcacc attctttcca cgctgccatc agcgggtgtg
    12361 ggtgcgattc tcgcattgct gctcttcaag ctcgatctca acgtcatcgg catcatcgga
    12421 atcattctgc tcatcggcat cgtgaagaag aacgccatca tgatgattga cttcgcgctc
    12481 gaagccgagc gcaaggaagg caagtcgccg gaagaatcta tctacgaggc atgtctgctg
    12541 cgcttccgtc ccatcatgat gaccacgatg gctgcgctgc tcggcggact gcccctggcc
    12601 ttcggcagcg gcatgggcgc ggagctgcgc cagccgctgg gcatcaccat tgtcggcgga
    12661 ttgatcttct cgcaggtatt gacgctctac accacgcccg tcgtctatct ctacttcgac
    12721 cggctgggtc agtggttcat gcgccggcgc gaacggcgct cacttgaaga acaggccgtc
    12781 cacgccgact aagaagaatc gccatgcact tgtccgcgcc attcattcga cggccagtag
    12841 caaccacgct gctgagcctt gctctgctgc ttgcaggtgc ggtggcgtac aatcttttgc
    12901 ctgtcgcctc tctgccggag attgaatttc ccaccatctc tgtttatgga ggactccctg
    12961 gcgccagtcc ggagaccgtg gctgccgcca ttgccacccc gctcgaacgg caatttggcc
    13021 gtatcgcggg cgtcactcag atgacttcct ccagttcgct gggcggaagt aacgtcaatc
    13081 ttcaattcga cctttcccgc gacatcaacg gcgcggcgcg cgacgtgcag gccgccatca
    13141 atgcagcgcg cagcaatctg cccgcgaaca tgccggagaa tccgacctac cgcaaaatca
    13201 atccgtcgga cgcgccaatc atgatcatcg cgctcacgtc agacgtgatg acccagggcc
    13261 agatgtacga cgcagcggac tcgatcctcg cccagaaaat ctcgcagatc cagggcgtcg
    13321 ggcaggtctt cgttggcggc agcgcgcagc cagccgtgcg cgtagaagtc aacccgatgc
    13381 agttaagctc ctacggcatt ggcctggagg gggtgcggac ggcgctcagc caggtaaaca
    13441 tgaaccttgc caaaggcttt ctcaaagctg gcgggcgcat gtccagcatc gctgacaatg
    13501 accagttgat gaaggccagc cagtacgctc cgctggtcat tggctacaat cccgccacgg
    13561 gtgctgcggt gcggttgagt gacatctccc aggtagtgga ctccgtctct gatgtgcata
    13621 ctgctggtct cgcaaacggc aagcgcgccg tgctggtcat tgtcttcaaa gttcccggcg
    13681 cgaacgtgat cagtaccgtg gaccgcattt acgctgcgat tccggctctg aaggcgtcca
    13741 tctcgccgct gattgacttc catgtggtcc tcgaccgtac catcaccatt cgcgcctcgg
    13801 tgctcgatgt cgaaatatcg ctcctgatca gcgttaccct ggtggtactc gtcgtctttc
    13861 ttttcctgcg aaatgtatgg gccaccatca tccccagcgt ggccgtgccg ctctcgctgg
    13921 tcggcacctt cggtgtgatg tacctgctgg ggtactcgct cgacaacctg tcactgatgg
    13981 ccctcaccat ctctacaggg tttgtggtgg acgacgccat tgtcgtcatc gagaacatta
    14041 cgcgctatct ggaaatgggc ctctctccat atgaagctgc ggtgaagggc tcaaaggaaa
    14101 tcggttttac ggtgctttcc atgagcactt cgctcattgc cgtcttcatt cccattctgc
    14161 tgatgggagg cgtcgtcggc aggctcttcc gcgaatttgc cgttacgttg agcgtcgcca
    14221 ttgccatgtc tctggtcgtc tcgctcacca ctacacccat gatgtgcgca aagttcctca
    14281 agccgcacaa ccagcaaaag catggccgca tttatatgct cagcgagcgc ttcttcaccg
    14341 gaatgctgaa gatgtatgac cacggtctcc gctgggtcat cgcgcataaa gtcccggtat
    14401 tgctgattac catcgccacc ttttttctga acatttattt gttcattctt gttcccaaag
    14461 gcttcttccc gcagcaggac acgggccgca tgatgggcat caccaacgct tctcaggaca
    14521 tctccttcga ggccatgcag caaaaacaag cgcagcttgc tgctatcgtg caggcggacc
    14581 ccggtgttca atctgtaatg gcctttgccg gaggcagcgg cggcggcgca accacaaacg
    14641 tgggccgcat gttcattact ctcaagccgc tggacgaacg gcatggtgtt agtgctgacc
    14701 agatcattaa ccgcctgcgc ccgaagcttg cgcacattcc cggagttact ctcgtcctcc
    14761 aggcggcgca ggacctcagc gttggcgcgc gcatgagcgc ggcgcagtac cagtacactc
    14821 tggaagacca ggacatcaag gagctgaacg aatgggcgcc caggctgctt gaaaaaatgc
    14881 gcaccatgcc cgagttgcgc gatgcttcca ctgaccagca ggaccagggc ctcgccgcga
    14941 accttgtcat tgaccgcgac acggctgccc gtctcggcat ttcaccttcc acaattgaca
    15001 acgtgctcta tgatgccttt ggtcagcggc aggtctcaac catgtacacg tcactgaacc
    15061 agtatcacgt ggtcatggaa gtggcgcagc cataccagcg tgatccgcag gcattaagca
    15121 acatctacat taagtccagc aatggtacga gcgtgccgct tacagcggtc acaaaattcg
    15181 agccgggacg cacatcgctg gccgtcaatc accagggaca gtttccggcc gtcacactca
    15241 gcttcaatct tgctcccggc gtttctctgg gacaggcggt cgtagcgttg cagaacgcag
    15301 aagcagacat gggtatgcct tccaccgtcc acggcagctt tcagggaaca gcgcaggcct
    15361 tccagtcctc gctttcaagc gaacccttcc tgattttgac ggcgctggcg acggtctata
    15421 tcgtgcttgg catcctgtac gaaagctatg tccatcccat caccattctt tccaccctgc
    15481 cgtcagcagg tgtcggtgcc atcctcgcct tgctcattac acataccgac ctcagcgtaa
    15541 tcgccattat cggaatcatt ctgctgatcg gcatcgtgaa gaaaaacgcc attatgatga
    15601 ttgattttgc cctcgccgcc gagcgcaatg aaggcaagtc gagcgatgaa gccatctatg
    15661 aagcctgcct gctgcgcttt cgtcccatca tgatgaccac catggccgcg ctgctcggcg
    15721 gtcttcccct tgccttgggg cgcgggactg gatcggaact tcgccagcct ctgggcatca
    15781 cgattgtcgg cggcctgatc gtttcacaaa tgatgacgct cttcactacc cctgtcgtgt
    15841 atctcttctt cgataagctg caactgcgcg tgcagcgctt cggcaagcgg ttcgagaa
//
LOCUS       DASRBV010000214         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_66946,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000214 DASRBV010000000
VERSION     DASRBV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66946"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFA02_08075"
     CDS             <1..592
                     /locus_tag="VFA02_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU09847.1"
                     /translation="KLMPRGIDTELFSPERRDRTPDDTDFVLGFVGRLSVEKNVALLA
                     RIREQLVAAGAEGFRFLIVGHGAEESWLRQQLPNAEFTGVLKGEALARAYANMDLFVF
                     PSHTDTFGNVVLEALASGVPAIVTPNGGPRYIVKEGVTGFVANDGDFATAIFRLMQNS
                     ELHEQMRRAARTDALGASWDSVFESVYQSYQTVRRN"
     gene            734..1321
                     /gene="ruvA"
                     /locus_tag="VFA02_08080"
     CDS             734..1321
                     /gene="ruvA"
                     /locus_tag="VFA02_08080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZU09848.1"
                     /translation="MIAHLRGRLFLKTPGRVIVEAGGVGYEVTISIPTFTALPAEGAE
                     VSLHIHTQVREDILALFGFLELDEKRLFERLITVSGVGPKLAVTILSGLNPERTVAAI
                     RGQDHVTLTRIPGVGKKLAERLVVELKDKLEDMAVAPVVAPAAPAAEDVLSALLNLGY
                     QRPAAQKAIEMAVAKNKSAGESFDDLFRMALQVIR"
     gene            complement(1311..1850)
                     /locus_tag="VFA02_08085"
     CDS             complement(1311..1850)
                     /locus_tag="VFA02_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZU09849.1"
                     /translation="MAHINSFVRIPLLAIRQHVRPLPRIGVLDEDRIRMRVWPNDIDF
                     NFHLNNSRYLTCMDYGRIHMMAANGILDHAIRGRWVPLVGSVDITYRRSLGLWARFEL
                     RTRTLGWDKKWFYIEQSFHCEAGLSAIAWVKGLFRNRQGNIPPQTVVDLVAPGTASPY
                     LPEELQQWNETIKKRLATE"
     gene            complement(1911..2099)
                     /gene="rpmB"
                     /locus_tag="VFA02_08090"
     CDS             complement(1911..2099)
                     /gene="rpmB"
                     /locus_tag="VFA02_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZU09850.1"
                     /translation="MAQVCEICGKGPQFGNNISHAHNVSRRRWNVNLRPVKAKVGGGS
                     KKLRVCTRCIKSGKVTKA"
     gene            2343..3242
                     /locus_tag="VFA02_08095"
     CDS             2343..3242
                     /locus_tag="VFA02_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU09851.1"
                     /translation="MKFAANILTASALVLATGIVMAQTAPAFHVDVRGKGKPMILIPG
                     LSSSGEVWDSTVARYQDRYRCYVLTLDGFAGQPRWIGPGDEFLNAVRDQLGAYIDQNH
                     LDHPVVVGHSLGGFLALALAEKYPDKVGPLVVVDSLPFSAHAWFNVDSAEAAKSVAAG
                     MRSRIAGETDEEYAAYVKSGASTRSMVTGDAGFARVKEWGLLSDKQAVADAMYDMMVA
                     DLRPELGRIHTPILVMGTWIGLTEVAHQEASPEFRSAVLKEFEEQYKNAPQAKIVLAD
                     HARHFIMYDDPQWFFTQMDAFLK"
     gene            3249..>4167
                     /locus_tag="VFA02_08100"
     CDS             3249..>4167
                     /locus_tag="VFA02_08100"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU09852.1"
                     /translation="MMMATVDQAFNTGAPAKPQSWRVLLSSFPPAYIAAQMLGWGLYA
                     AVSFPLVEFGGYYRHASDALLSVVLFSFYGFIATHVLRAVCHQRGWADMPFIRLAPRT
                     FLAAALSGLWMAGCLVVINSMLLRLFSAHGRDAWMVLLYMMPNASVMVLFWEFFYFLA
                     KTIERRRMAEMQGLQLQLLAREAQHRALSSQLQPHFLFNCLNSLRSLIVEDPSRAREM
                     VTDLAGLLRYSLDHDMVTRVTLREELAALDRYLALEGIRFEDRLRVRRQISLETMNAL
                     VPPMMVQTLAENAIKHGIAGRSKGGEIVIE"
BASE COUNT          849 a         1068 c         1292 g          958 t
ORIGIN      
        1 taagctgatg ccccgcggaa ttgatacgga gttgttttca cctgagcgcc gcgaccgcac
       61 gcctgacgat acagattttg tgcttgggtt tgtggggcgc ctctcagtcg aaaagaacgt
      121 agcgctattg gcgcggattc gggagcagct tgtggccgca ggcgcggaag gattccgctt
      181 tttgattgtc ggccacgggg cggaagaatc ctggctgcgc cagcaattgc cgaatgctga
      241 gtttaccggg gtcttaaagg gcgaggcgct ggcgcgcgct tacgccaata tggacctttt
      301 tgtcttcccc tcgcataccg acactttcgg gaacgtggtg ctggaggcgc ttgcctccgg
      361 agtgcctgcg attgtgaccc cgaatggtgg accgcgttac atcgtgaagg aaggcgtaac
      421 cggatttgtc gccaatgacg gagactttgc taccgccatc tttcgcctga tgcaaaattc
      481 agagctacac gaacaaatgc gcagggccgc gcgcacggat gcgctgggcg cttcgtggga
      541 ctcggtattt gagagtgtgt atcagagtta ccagaccgtc agaagaaatt gataagcgca
      601 ggccacggtg ttttacctga acggccctgc taaaacatga ccgccagagg aacgtaaagt
      661 aaacgtatag agccgcagag gcatatagga tccggggagc taacgctcgg cttttttgct
      721 ttacaatcgc ggcatgattg cgcatttgcg gggacgtctt tttttgaaga cgccggggcg
      781 ggtgattgtt gaggccggtg gcgtaggtta tgaggtcacc atcagcattc ctacgtttac
      841 ggcgctcccg gcagaaggag cggaggtatc gctgcacatc catacgcagg tgcgcgagga
      901 catccttgcg ctatttggct ttctggaact tgacgaaaag cgtttgtttg agcggctgat
      961 taccgtttcg ggcgtagggc caaaactggc tgtgaccatt ctgagcggtc tgaatccaga
     1021 gcgcacggtt gcggccatcc gggggcagga ccacgttaca ctgacgcgta tcccgggcgt
     1081 gggaaaaaag ctggctgagc ggcttgtagt ggaattgaaa gacaagctgg aagacatggc
     1141 tgtggcccct gtggttgctc cggctgctcc ggctgcggaa gatgtgcttt cggcgctgct
     1201 gaacctgggg taccagcgac ccgctgcgca gaaggcgatc gaaatggctg tggcgaagaa
     1261 caagtctgcg ggcgagagct tcgatgatct gttccgaatg gcgctgcagg tcattcggta
     1321 gccagtctct tctttatggt ttcattccac tgctgcaatt cttccggcaa gtagggcgag
     1381 gccgttcccg gcgcgacgag gtccacgacg gtttgcggcg ggatgtttcc ctggcgatta
     1441 cggaagagac ctttgaccca ggcgatggcg gagagtccgg cttcacagtg aaagctctgc
     1501 tcgatataga accatttctt gtcccatccg agagtgcgag tgcgcagctc aaatctggcc
     1561 cagaggccga gcgagcggcg gtaggtgatg tccaccgagc cgaccagcgg cacccagcgt
     1621 ccgcgaattg cgtggtcgag gatgccattg gcggccatca tgtggatacg gccgtagtcc
     1681 atgcaggtga gatagcgcga gttgttcagg tggaaattga aatcaatatc attcggccag
     1741 acacgcatcc ggatgcggtc ttcgtcgagc actccaatgc ggggtaatgg ccgaacgtgc
     1801 tgacgaatgg caagcagcgg aatacggacg aagctgttga tgtgcgccat ctgcaaagag
     1861 aaaaccccgg aacaagccgg ggttttttat tatccagaat ttgttaaaaa ctacgccttg
     1921 gttaccttgc cgctcttgat gcatctggtg cagacacgca gcttctttga gccgccaccc
     1981 accttcgcct ttaccgggcg gagattgacg ttccagcgcc gcctgctgac gttatgcgcg
     2041 tgcgagatat tgtttccaaa ttgcgggcct ttgccgcaga tttcacaaac ctgagccatg
     2101 ataaaactcc agaatccctg aacttttcgc gcaaaccagt acccgccgga aactgcgttc
     2161 ttcgcaatgt gcgagaaggg cgggcaagca gacagaggaa tgcccgggct ggtgccgaat
     2221 ctttagttta ccccaaggca ggcgctgtga gcaaccgctt gtcacgagga tgtgccgctc
     2281 gtctcaattt gcaccatccg cgatcgcttc cgacgtagtg tttgtttgcg gaggaacatt
     2341 ctatgaaatt cgctgcaaat attctgactg cgagtgcgct tgttctggcc acgggcattg
     2401 tgatggcaca gacggcgcca gctttccatg tagatgtgag gggcaaaggc aaacctatga
     2461 ttctgattcc ggggctttca tcgtcgggcg aagtatggga ttcgacggtg gcacggtatc
     2521 aggaccggta ccgttgttat gtgcttacgc tggacgggtt tgcagggcag cctcgctgga
     2581 tcgggccggg cgatgaattc ctgaatgccg tgcgcgacca actgggggca tatatagacc
     2641 agaaccatct tgatcatccg gtcgttgtgg gccattcact gggaggattt ctcgcgctgg
     2701 cattggcaga gaagtatccc gacaaagtgg ggccgctggt cgttgtggat tcgctgccat
     2761 tttcagcaca cgcatggttt aatgtggact cggccgaagc ggcaaagagt gtggctgcgg
     2821 gcatgcgctc gcgcatcgct ggtgaaacag atgaggaata tgcggcgtat gtgaagagcg
     2881 gggcctctac gcgttccatg gtcacaggtg atgctggttt tgcgcgagtc aaggaatggg
     2941 gcctgctttc tgacaagcag gcggtggccg atgcaatgta tgacatgatg gtggcggacc
     3001 tgcgtcctga actgggccgc attcacactc ccattctggt gatgggcacg tggattggcc
     3061 tgaccgaagt agcgcaccag gaggcgagtc cggaattccg cagcgctgta ttgaaggaat
     3121 ttgaggagca gtataaaaat gctccgcagg caaagattgt gcttgcagac cacgcgcgcc
     3181 acttcatcat gtacgacgat ccgcagtggt tctttaccca gatggatgcg tttctgaaat
     3241 aaagtgcgat gatgatggca accgtggacc aggcattcaa taccggcgcg ccggcaaaac
     3301 cgcaaagctg gcgcgtcctg ctgtcgtcat tcccgcctgc ttatattgcg gcgcagatgc
     3361 tgggatgggg actgtatgct gccgtcagct tccctctggt cgagttcgga gggtattaca
     3421 ggcacgcatc cgatgcgctc ctgtcggtcg tgctcttttc cttctatgga tttatcgcca
     3481 cccacgtgtt acgggctgtc tgtcatcagc gtggctgggc ggacatgcca ttcatccggc
     3541 tcgcgccccg gacgtttttg gctgcggccc tgtccggcct gtggatggcc ggttgcctgg
     3601 tggtcatcaa tagtatgctt ctcagactgt tttctgcgca tggccgcgat gcctggatgg
     3661 ttctcttgta catgatgcca aacgccagcg tcatggtcct gttttgggag ttcttctatt
     3721 ttctggccaa aacgatcgaa cgcaggcgta tggcagagat gcagggcctg caattgcaac
     3781 tgctggcgcg ggaggcgcag caccgagcct tgtcatcgca gctacagccg cactttcttt
     3841 ttaattgcct gaattcattg cggtctctta ttgtggaaga cccgtctcgt gcaagagaga
     3901 tggtgacaga cctggcggga ctgctgcgct attcgctcga ccatgacatg gtaacgcgcg
     3961 tgacactgcg cgaggaactg gcggcgctcg accgctatct ggcgcttgaa ggcataaggt
     4021 tcgaggaccg cctgcgcgtc cggcgtcaga tttctctgga aaccatgaat gcgctggtac
     4081 cgcccatgat ggtgcagaca cttgcggaaa atgccatcaa gcacggtatt gcaggacgca
     4141 gtaaaggcgg agagatcgtc atcgagg
//
LOCUS       DASRBV010000215         7535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_317262, whole genome shotgun sequence.
ACCESSION   DASRBV010000215 DASRBV010000000
VERSION     DASRBV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317262"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VFA02_08105"
     CDS             complement(<1..1046)
                     /locus_tag="VFA02_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZU09853.1"
                     /translation="MKLLLAFVLLPVALFAQAPKSGPWEMQNSGTTAGLRGIHAVGGG
                     VVWASGMNGTVLRTEDGGYMWQSCAVPSGAENLDFRGIWAWDANTAIVMSSGPGGQSR
                     LYETTDGCSHWASLYTNPDKDGFWDAIVFQNKRRGFLLGDPVDGKFVFLTTEDGGQHW
                     VRSEGAGLEADSKAKGAFAASNSSLLGRPEEPVMFGTGGAFIYSQSFTGTIDFSSKPG
                     TPAKMAEQWKRTPVPLANAGDAAGIFSLGYHSGNGPILVAVGGDYTKPNESAGTAAWS
                     SDGGAHWTAAIKPPHGYRSAVAWDADAKAWIAVGTNGSDISYDEGKTWQPLDDGNWNA
                     LSLPWVVGPKGRIA"
     gene            complement(1051..1392)
                     /locus_tag="VFA02_08110"
     CDS             complement(1051..1392)
                     /locus_tag="VFA02_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09854.1"
                     /translation="MQRNICTNVLLLIIALALVIIAVRTYTAPRAVQAQSLSTAPYPY
                     FFEPGTQMLRAPDGSKQLYGRVVIDMRTGKVWGFPTNTPDTYPTNPVDNKPQVSHPFY
                     LGRFAFEDINQ"
     gene            complement(1509..2423)
                     /locus_tag="VFA02_08115"
     CDS             complement(1509..2423)
                     /locus_tag="VFA02_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZU09855.1"
                     /translation="MAFYLGFDAGGTKTDCALADETRILARAQSGSIKPLRVSAEQAE
                     ANMRDLLAGISRQSGVDLKQVAVSCVGTAGVRLPQTRQWMEQIISKYAGGEILVCGDE
                     ATALDAAFPGEAGVLVIGGTGSNILGRTSTGETFNIGGWGPVLGDEGSGYWIGLEALK
                     AACRAHDFQRPTMILERVMRFWSAPAIGDVVNKAHSTPAPDFSQLAPLVVECAEAGDV
                     VALEVLELGGRILGECATHAVRRLRALEPDAPVPSVAYVGGILKSVHFVRESMIETIH
                     QSLPMVKVLPEAVDPVTGALWRARNQVR"
     gene            complement(2444..3712)
                     /locus_tag="VFA02_08120"
     CDS             complement(2444..3712)
                     /locus_tag="VFA02_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HZU09856.1"
                     /translation="MKRLWLLLFSLVLAASAAAQQIAPVITVDNGPNALVQEQKHYVV
                     LVSLDGFRYDYAKKYGATHLLDLAKHGASAPDGMLPSYPSLTFPNHYTLVTGLYPEHH
                     GIVAMSFYDPQRQERYSYNDPKSSTDGSWYGGTPLWVLAEQQGMRSACFFWPGSEAAI
                     RGVRPSYYLHFDDKFPDGKRIEQVLAWLRLPEDRRPHFITLYFSNVDHAGHEFGPDSP
                     QVAAAVQHVDELIGKLHSGLDALHMPIDLIVVSDHGMAKREGGWINLDQYADLSRFTT
                     IGSMLYANADADAERAYQKLKAADGKFVVYRRAHMPAYLHYDSNPRIGDPVIVAKGPY
                     AIRAHGPPPGEQDRPPNMGVHGYDPREMPEMKAIFYAVGPDIRPGVTLKPFENVNVYP
                     FIAKILQLPTPPTDGSLNILSNALTEKALQ"
     gene            3808..4044
                     /locus_tag="VFA02_08125"
     CDS             3808..4044
                     /locus_tag="VFA02_08125"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZU09857.1"
                     /translation="MKRTNLVLDAHLLEEATRVLHAKTYSAAVNTALAEVLRVKRIQA
                     IPQFFGMGLWEGDLSEMRGDTPQARKRPRQRKRP"
     gene            4041..4430
                     /locus_tag="VFA02_08130"
     CDS             4041..4430
                     /locus_tag="VFA02_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZU09858.1"
                     /translation="MILVDTSVWIELLRSGQRTNITEEHLFNFVTCGPVLQEVLQGLR
                     QTPASNAFRLALLALPVISNPLPRSLFIEAAEIYRTGRAKGHTIRSSVDCLIAAIAIE
                     NHVPVWHRHRDFDAIAKFTSLCIFQPR"
     gene            4526..7342
                     /locus_tag="VFA02_08135"
     CDS             4526..7342
                     /locus_tag="VFA02_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZU09859.1"
                     /translation="MSRRHFAVLGVLAMAASTMTGIAETAKLKVPEIKYESYTLPNGL
                     QVILSEDHRLPIVGVDIWYHVGPMNERAGRTGFAHLFEHMMFEGSKHVGEKAHFKYLE
                     AAGASDINGTTSFDRTNYFETLPSNQLELALWLESDRMGFLLDTLDREKLTNQRDVVR
                     NERRQSIENRPYGLVDEALMHELFPKDHPYYADVMGSHADIEAARLEDVREFFKLYYA
                     PNNATLAIAGDFDPAKTKALIEKYFDPIPKGPDVPKVNVATPPITSEKRLTVTDKVQL
                     PKVIFGWLTPPAFAPGDADGDVASYILGGGKTSRLYQKLVYQQQIAQSVACYNQSQAL
                     QSWTSCELVARPGVSAEQLEKAANDVINDFLQNGPTQEELDRARIVTQSGRIRGLQRV
                     GGFGGIADTLNYYNQYTGDPGYLPKDLARYDALTPASVQKFAQAHLKQDQRVTVYGIP
                     GEKVIHDVPRSPADTDANVKITPEYSAEFEASQSWRKTPPSAGPQPKLSLPEPKVFTL
                     SNGLKVYLVEWHNLPVFSASLVTLGGSEGDSTKKPGVAGFTAAMLTEGTTNRSATQVA
                     DDADKIGARLNKNATNDLAYVQISALSNVADPALDLLSDVVLHPAFSSEEIERIRKQR
                     LTSLLQLKDQPVQIAFSVANRAIYGDNSPYGYRSIGTEAALKTITRDDLADYWKQHYA
                     PSDSALVFAGDLTEKRARDLAEKYFGSWTSKGTASQPPAAPAPPQRHVVLVDMPGAPQ
                     TAIWAAGVGVPRSTPDYAPLNVVNTMLGGLFSSRINMNLREEHGYTYGAFSSFQFMRG
                     AGPFFAGASVRTDVTGPAMHELLFELERIRTNPLSADELKMSRDYLVRSLPGDFETVE
                     ETSGQIADIFTYDLPLDYYRTYPAKIEAVTSEQATAAALKYIHPENMVFVAVGDKAKI
                     QPQIEKLNLGPMEEWTQDAEPVKK"
BASE COUNT         1632 a         2189 c         2138 g         1576 t
ORIGIN      
        1 ttggcaatcc tgccctttgg cccaaccacc cagggcagac tgagggcatt ccagttgccg
       61 tcatccagcg gttgccatgt ctttccctcg tcataactga tgtccgaacc gttggtcccc
      121 accgctatcc aggctttggc atccgcatcc caagccactg ccgagcgata gccatggggc
      181 ggcttgatgg cggccgtcca gtgcgcgccg ccgtcactac tccaggccgc ggtcccggcg
      241 gattcgttgg gtttagtgta gtcgccgcct acggcgacga gaatggggcc gttgccagag
      301 tgatatccaa gagaaaaaat acctgccgca tcaccagcgt ttgcgagtgg aacgggagtg
      361 cgcttccact gttcggccat tttagccgga gttcccggtt tggaagaaaa atctatagtg
      421 cccgtgaatg actggctata gatgaaggcc cctcctgtgc cgaacatgac tggctcctcg
      481 ggcctgccga gcagggaaga attgctcgct gcgaaagcgc cctttgcctt gctgtctgct
      541 tccagaccgg ctccttctga gcggacccaa tgttgccctc cgtcttcagt tgtcaggaag
      601 acaaattttc catccaccgg atcgccaaga agaaagcccc tgcgcttgtt ctggaagacg
      661 attgcgtccc agaatccatc tttgtcgggg ttggtataaa gcgatgccca atgcgagcac
      721 ccgtcggtgg tttcatagag ccgcgactgg ccgccggggc cgctggacat cacaatggcc
      781 gtgttcgcgt cccatgccca gatgccgcga aagtccaggt tttccgctcc ggaaggtaca
      841 gcgcagctct gccacatata tccgccatct tccgtgcgca ggaccgttcc attcatcccg
      901 ctggcccaga ccactccccc gcccacggca tgaatgccgc gcagacccgc cgtggtgccg
      961 ctgttctgca tctcccatgg gccagacttc ggcgcctgag caaaaagagc gaccgggagc
     1021 agcacgaagg ccaagagcag tttcattgtc ttactggttg atgtcttcaa aggcgaacct
     1081 gcccagatag aaaggatgcg aaacctgcgg tttgttgtct accggatttg tgggatatgt
     1141 atcaggcgta ttggtgggaa agccccagac tttacctgtc cgcatatcaa tcacaactcg
     1201 cccataaagc tgcttgctgc cgtcgggcgc gcgcagcatt tgtgttcccg gctcaaagaa
     1261 atagggatag ggcgctgtag ataaggattg tgcctggacg gcccggggag cggtataagt
     1321 gcggaccgcg atgatgacca gggcaagtgc tatgatcagc agcagaacat tcgtgcagat
     1381 gtttcgttgc atgaatcagc ttcctccgtg ttctggattg taacggcttc ttattcgccc
     1441 cgcagcgcct tcgccgggtc cacggcagca gcacaacgcg ccggataagt agtgcaaatg
     1501 aaaaccagct acctgacctg gttccgcgcc ctccacagcg ctccggtgac tgggtctaca
     1561 gcttcaggga gcactttgac catcggcaaa ctctgatgga tggtctcaat catgctttcg
     1621 cgcacgaaat gcacgctttt caggatcccc cccacataag ccacgctggg caccggagca
     1681 tctggttcga gggcgcgcag acggcggacg gcgtgtgttg cgcattcgcc caggatgcgc
     1741 ccgccaagct ccagcacttc aagggcgacc acatcgccgg cctcggcgca ttcgacgaca
     1801 agcggggcaa gctgcgaaaa atcaggcgcg ggcgtgctat gggccttgtt gacgacatcg
     1861 ccaatcgcag gggcggacca gaagcgcatc acacgctcca gaatcatggt gggccgctgg
     1921 aaatcatggg cgcggcaggc agccttcagg gcttcgaggc cgatccagta gcctgagcct
     1981 tcatcgccca gcacagggcc ccatccacct atgttaaagg tttcgcctgt gctggtgcgg
     2041 cccagaatat tggagccagt cccaccgatg acgagcacgc ctgcctcgcc gggaaaggcc
     2101 gcatcgagcg cggtggcttc atcgccgcag acgagaattt caccgcctgc gtactttgag
     2161 atgatctgct ccatccactg ccgtgtctgc gggagacgca cgcccgccgt gccgacgcag
     2221 gagacggcga cctgtttgag gtctactccg ctctgacgcg agatcccggc aagcagatcg
     2281 cgcatgtttg cctctgcctg ttcagcagag acgcgcagcg gtttgatgct tccgctctgc
     2341 gcgcgggcga gaatgcgggt ttcgtcggca agcgcgcagt cggtcttggt gccgcctgcg
     2401 tcgaatccta aataaaatgc catcgttcta cttttctcgt ttgttattga agagcttttt
     2461 cggttaatgc gttggaaaga atattcagac tgccatccgt gggtggggtg ggcaattgca
     2521 ggatttttgc aatgaaggga tacacattca cattttcaaa aggcttcagc gtcactccgg
     2581 gacggatgtc cggccctaca gcatagaaga tggccttcat ttccggcatt tcgcgcggat
     2641 catatccatg cacacccatg ttcggcggac ggtcctgctc accgggcgga gggccatgtg
     2701 cgcgaattgc atacgggcct tttgccacaa tcaccggatc gcctatgcgc gggttgctgt
     2761 cgtagtgcaa atatgccggc atgtgtgcgc ggcgatagac cacgaatttt ccatcggcgg
     2821 ccttcaattt ttgataggcc cgttcggcgt cggcatctgc attggcgtaa agcatagagc
     2881 caatggtagt gaaacgcgaa agatcggcat actggtcgag attgatccag ccgccttcac
     2941 gctttgccat gccgtggtcg ctgacgacga tgaggtcaat gggcatatgc agagcgtcga
     3001 ggccggagtg aagcttgccg ataagctcat ccacatgctg cactgccgcg gcaacctgcg
     3061 gcgaatccgg cccaaactca tgtccggcgt ggtcaacgtt ggagaagtat agcgtaatga
     3121 aatgggggcg gcggtcttcg ggcaatctaa gccacgcgag tacctgttcg atgcgcttcc
     3181 cgtcgggaaa cttatcgtcg aagtgcagat aatagctggg gcgcacgccg cgaattgccg
     3241 cctctgaccc cggccagaag aagcaggccg agcgcattcc ctgctgttcg gccaggaccc
     3301 ataaaggagt gcccccatac caggagccgt cggtgctgct tttcgggtcg ttatacgagt
     3361 aacgctcctg ccgctgtgga tcataaaagc tcatggcaac gatgccgtgg tgctccggat
     3421 agagtcctgt caccagcgtg tagtggttgg gaaacgtcag cgaggggtag gacgggagca
     3481 ttccatccgg ggcgctggcg ccgtgttttg ccagatccag caaatgcgta gcgccatatt
     3541 tcttcgcata gtcgtagcga aagccgtcga gcgaaaccag gacgacgtag tgcttctgct
     3601 cctggacgag ggcgttcggg ccattatcta cagtaatgac cggggcaatc tgctgcgcag
     3661 ccgcagatgc cgccaggacc agtgagaaga gaagcagcca gaggcgtttc atgggatttc
     3721 cggacaaccg tccttcatca gcttaatgga tggagaccac agaccgggaa acatcgcggg
     3781 cattttgagt tcatgtatca tttacacatg aaacgcacaa atcttgtgtt agatgcacat
     3841 ctgctcgaag aagccacaag ggtgctccat gcaaagacct actcggctgc tgtgaacacc
     3901 gctttggctg aagtcctccg cgttaagcgc atacaagcca ttccgcagtt tttcggaatg
     3961 ggcctctggg aaggagacct ctcggaaatg cgcggggaca cgccccaggc aaggaagcgg
     4021 cccaggcagc ggaaacgccc atgatcctgg tagacacctc agtctggatc gagctgttgc
     4081 gcagtggtca gcgcaccaac attacggaag aacacctgtt caatttcgtc acctgcgggc
     4141 ccgttttgca ggaagtcctt cagggactac ggcagacacc cgcaagcaat gcgtttcgcc
     4201 tggcattgct ggcgcttccc gttataagca atcccctgcc tcgctcatta tttatcgagg
     4261 ctgcggaaat ctaccgcact ggcagggcca aagggcatac catccgctct tcggtggact
     4321 gccttattgc ggcaatcgca atcgaaaatc atgtacctgt gtggcataga cacagagatt
     4381 tcgacgcaat tgcgaaattc acttcgcttt gcattttcca gccccggtag tcccgccgga
     4441 accatcactc tgcggcctgg cctgatatca tcccaatttc agggctgttt caaacgcatc
     4501 tttgagggga ggcgcgtttt taatcatgtc tcggagacat tttgctgttt taggagtact
     4561 cgctatggct gcttccacca tgaccggcat tgcagaaacc gcaaagctca aggtgccgga
     4621 gatcaaatat gaaagttaca ctttgcccaa tgggcttcag gtcattctgt cagaagacca
     4681 tcgtctgccg attgtcggag tggacatctg gtaccacgtc ggcccgatga acgagcgggc
     4741 cggacgcacc ggctttgcgc atctgtttga gcacatgatg tttgaaggct cgaagcacgt
     4801 gggcgaaaag gcccatttta aatacctgga ggccgcaggc gcctcggaca tcaacggcac
     4861 cacctccttt gaccgtacga actactttga gacgctgcct tcgaaccagt tggaactggc
     4921 cctttggctg gaaagcgacc gcatgggatt tctgctcgat acgctcgacc gcgagaagct
     4981 gaccaaccag cgggatgtcg tcaggaatga gcgccgtcag agcattgaga accgccccta
     5041 tggtctggtg gatgaggcat tgatgcacga actcttcccc aaagaccatc cttactatgc
     5101 cgatgtgatg ggttcgcacg cagacattga agcagcgcga ctcgaagatg tgcgcgagtt
     5161 cttcaagctg tattacgcgc caaacaacgc cacgcttgcg attgccggag actttgaccc
     5221 ggcaaagaca aaagccctca ttgagaaata tttcgatccg attcccaaag gcccggatgt
     5281 tccgaaggta aatgtagcca caccacccat aacctcagaa aaacgcctta ccgttaccga
     5341 taaggtccag ctccctaagg tgatctttgg atggctcacg cctcctgcct ttgctcccgg
     5401 agatgcggat ggcgacgtgg cctcttatat cctggggggt ggcaagacca gccggctcta
     5461 ccagaagctc gtctatcaac agcagatcgc gcagtctgtg gcgtgctaca accagtcgca
     5521 ggcgctccag tcctggacat cctgcgagtt agtggcgcgt cccggcgtca gcgccgaaca
     5581 gcttgaaaag gcggcaaacg acgtcatcaa cgacttcctc cagaatggcc ctacccagga
     5641 agaactggac cgcgcccgca tcgtcaccca gtccggcagg attcgtggtc ttcagcgtgt
     5701 tggaggtttt ggcggtattg ccgatacgct gaattactat aaccagtaca ccggcgaccc
     5761 cggctatctg ccgaaggacc ttgcgcgcta tgacgcgctg actcctgcga gcgtgcagaa
     5821 gttcgcgcag gcgcatctga agcaagacca gcgcgtgact gtttatggca tcccgggaga
     5881 gaaggtcatc catgatgttc cccgcagtcc ggcagacacc gatgccaacg taaagatcac
     5941 gcctgaatat tctgcggaat ttgaagcttc tcagtcatgg cggaaaacac cgccttctgc
     6001 cgggccgcag ccaaagctca gcctgcccga gccgaaggtc tttacgctct cgaatggcct
     6061 gaaggtttat ctggtggaat ggcacaatct tccggtcttc agcgcgtcac tggtcacgct
     6121 gggggggagt gaaggagatt ctacgaaaaa gcccggagtg gctggattta ccgcagctat
     6181 gctgacggaa ggcactacga accgcagcgc cacacaggtc gccgacgacg cggataaaat
     6241 tggcgcaagg ctgaacaaga acgccaccaa cgatcttgct tacgtgcaga tcagcgcgtt
     6301 gagcaacgtc gcagaccctg cccttgacct gctatcggac gtcgttcttc atccggcctt
     6361 cagcagcgaa gagattgagc gcatccgcaa gcagcgcctc acctcgctcc tgcagttgaa
     6421 agaccagccg gtgcagattg cattttctgt cgccaaccgg gccatctacg gcgacaacag
     6481 tccctatgga taccgcagca tcggaacaga agcagcatta aagaccatca cgcgcgatga
     6541 tctggcggat tactggaagc agcactatgc gccgtcggat tcggcactgg tctttgccgg
     6601 tgatctgacg gaaaagcggg cgcgcgatct ggccgaaaaa tactttggtt cctggacatc
     6661 gaaagggaca gcatcgcagc cgcccgccgc acctgctccg ccgcagcgcc atgtggtgct
     6721 ggtagacatg cccggtgcgc cgcaaacggc catctgggct gcaggcgtcg gtgtgccgcg
     6781 ctccactccg gactacgcgc cgctgaacgt ggtcaacacc atgcttggcg gactcttctc
     6841 cagccgcatc aatatgaacc tgcgtgaaga gcacggatat acctacggcg ccttcagctc
     6901 gttccagttc atgcgcggcg caggaccgtt ttttgccggg gcttccgtgc gtactgacgt
     6961 gaccggacca gccatgcacg agctgctttt tgagcttgag cgcatccgga caaacccgct
     7021 ctccgcagac gagctgaaaa tgtccaggga ctacctggtc cgctcgcttc cgggtgattt
     7081 tgagacggtg gaagagacca gcgggcagat tgcggacatc ttcacctatg acctgccgct
     7141 tgattactac cggacctatc cggcaaagat cgaggcagtc acttcagagc aggccacagc
     7201 tgccgctctc aaatatatcc atccggaaaa catggtcttt gtcgcagtag gagacaaggc
     7261 aaaaatccag ccccagattg agaagctcaa tctcgggccg atggaggagt ggacacagga
     7321 tgccgaacct gtgaagaagt aactgtaaac ggggccccct tgtccagcct gaaagggggt
     7381 tttccccagc accccggtgc tgtgcgacaa taaaaagaac ggaggaagtt cccatgcgcc
     7441 ccgcgtgtgg gataggattc cattatgctg gacaatctgc ttaaacccga gcacctgctg
     7501 ctgattcttg ccattgcgtt gctgatcttt ggacc
//
LOCUS       DASRBV010000216         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_677328, whole genome shotgun sequence.
ACCESSION   DASRBV010000216 DASRBV010000000
VERSION     DASRBV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677328"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFA02_08140"
     CDS             <1..1101
                     /locus_tag="VFA02_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09860.1"
                     /translation="WLQYSKRLFDAGLINLIDIVSMHPYQAGPPEYSDNGMTYIEMAQ
                     RLENLASSYGEKKPVWSTEANWLIGPAGTPGVTAPDVNEHQQSQYVVRVNLLSLGIHV
                     PYFVHSPFFTGFHREVLVDSLSSYAAMTSILSDAQNAKILQLPEGLYGISASTKSGTV
                     LALWSTRSTGATVHVSGLRNATMQDMYGNPMSYTDNPTVNSSPIYITGTGVPTVSAAA
                     TASGMLAMAAAPAAQAVALAPLQSWTKGQKTQLQNSQNGVHVFASGVKYDNQIVSPVF
                     PVSPNSCYVIRPELIVHQGGVGIVVSDPDIHKNLRADYMYAYTGNDRYTPAIRIETQN
                     HTHLQLIFQAGNAHDPANTEFDLTGVQINRCH"
     gene            1412..2050
                     /locus_tag="VFA02_08145"
     CDS             1412..2050
                     /locus_tag="VFA02_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09861.1"
                     /translation="MAASPASVGVLVTNGQAMVDGATVHGNSTLFQGSVVKADEITSD
                     LRFADGANLVLQPGAAAKVYRDHAELQQGTAMQRGANGHALVADGLRVTSLEPNGKVT
                     VAVQDNRHVEMLANDGAAEVRTPTGELVARLEPGKPLTFNIREVAKGTNVNAYAGGKG
                     QAGGGAAPAAYGTVWTTGSVIFVAAVAAGGTVLGLAAAGELGNGPSHPVSTP"
     gene            complement(2091..>2445)
                     /locus_tag="VFA02_08150"
     CDS             complement(2091..>2445)
                     /locus_tag="VFA02_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09862.1"
                     /translation="MVKARPFTGFGLGCWPAVYPAFATFDPGAFVNQAHSDWLQWIAE
                     GGIPTGIAMVALFVWFIRPAWKSIWAIGGIAIFVHAAFDYPFSRPAIGALPFLVLAMA
                     VAWVEEWDAKQRRSA"
BASE COUNT          620 a          623 c          648 g          554 t
ORIGIN      
        1 tggcttcaat atagcaagcg ccttttcgac gccggtttaa tcaatttaat tgatattgtt
       61 tccatgcatc cttatcaggc cgggccgccg gagtacagtg acaatggcat gacctacata
      121 gagatggcac agcgtctgga gaacctggct tcttcctatg gtgaaaagaa acctgtgtgg
      181 agcacagaag cgaactggct gattggtcct gccgggaccc ccggcgtgac cgcgcctgac
      241 gttaacgaac atcaacaatc tcaatacgtg gtgcgtgtca atttactctc gcttggaata
      301 cacgtgccat attttgtgca ttcgcctttt ttcactggct tccatagaga agtgctggtg
      361 gattctttgt cctcttacgc tgcgatgacc tcgattctct ctgatgcgca gaatgcaaag
      421 atactgcaac tgccggaagg actctatggc atttcagcat caacgaagtc gggtactgtg
      481 cttgcattat ggtccacacg ctcgacaggc gcgacggtcc atgtgagcgg gctacgaaat
      541 gcaacgatgc aggacatgta cggtaatcct atgagttaca ctgataaccc aacggtcaac
      601 agcagcccta tctacataac cgggactgga gttccaacgg tttcagcagc tgctacagct
      661 tcaggaatgc tggccatggc cgctgctccg gcagcgcagg cggttgcttt ggcgccgctg
      721 cagagctgga ccaaaggcca aaagacccaa ctgcaaaata gccaaaatgg tgttcacgtt
      781 tttgcttccg gcgtgaagta tgacaatcaa atagtttcac cggtctttcc tgtctctcca
      841 aacagttgtt acgtaattcg cccagagtta attgtccatc agggtggagt ggggatcgtt
      901 gtttccgatc cggacattca caaaaacctg agagcggact acatgtatgc atacacggga
      961 aatgacagat acacgcctgc gatcagaatc gagactcaaa atcatacaca cctgcagctc
     1021 atctttcagg cgggcaatgc ccatgatccg gcaaatactg aattcgactt aactggcgtg
     1081 cagataaacc ggtgtcacta ataactttca tattattaaa accaatttaa actcacataa
     1141 ctcgctgaat tgacttacat tgcgcataag cccagaataa tgagcgtgac atcatttttg
     1201 ggatgagatt aacatcctgg tcgtactctg caggcatcag aagtagctgt aaagtcaggt
     1261 tgattttagc gttgcaatgg attttgcagc aggggtaaaa tcacctcctg attggtcgtt
     1321 gttccgaatc ccccttatct cagggaggta aaggatgaat ctgaaggccc attcaatcgt
     1381 atccctgttc acatgcagtt gcatggtgtg catggctgcg tcgcccgcaa gcgtaggagt
     1441 actggtgacg aatggtcagg caatggtaga cggcgcaacc gtgcatggga actcgaccct
     1501 gtttcagggg agcgtagtga aagccgatga aatcacgtca gaccttcggt ttgcagatgg
     1561 agcaaatctc gtgcttcagc ctggagcggc cgcaaaggtc tatcgtgacc atgccgagtt
     1621 gcagcaaggg acagcgatgc aacgtggagc gaacggtcat gcattggtgg ccgatggact
     1681 gcgagtcaca tcccttgaac cgaatggcaa agtgacggtc gccgtccagg acaaccggca
     1741 tgtagaaatg ctggccaatg acggtgctgc ggaagtccgc acacctacag gcgagctggt
     1801 cgcccgcttg gaaccgggca agcctctcac ctttaacatc cgtgaggtgg ccaaaggaac
     1861 aaacgtgaat gcctacgcag gcggcaaagg gcaggcggga ggcggagccg ctccggcagc
     1921 ttatggtacc gtctggacta cgggaagcgt tatttttgtg gcagcggtgg ccgctggggg
     1981 aacggttctg ggactggcag ctgcgggcga actgggtaat ggcccgtcac accccgtaag
     2041 cacaccgtaa ttgaaatctg gtcctgaatt tctccagaaa aggagcgcag tcatgcgctc
     2101 cttctttgtt ttgcgtccca ttcctctacc caagccacag ccattgctaa aacgagaaat
     2161 ggaagggccc ctatggcggg ccttgaaaat ggataatcaa aggcagcatg gacgaaaatc
     2221 gcaatgcctc ctattgccca gatggatttc catgccggcc tgataaacca gacaaaaaga
     2281 gccaccattg cgattcccgt tggaataccg ccttctgcaa tccactggag ccagtcggaa
     2341 tgtgcctggt tcacaaacgc gccaggatcg aaggtggcaa aggcaggata taccgcaggc
     2401 cagcatccca aaccaaagcc agtgaaaggc ctggctttca ccatg
//
LOCUS       DASRBV010000217         9561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_573321, whole genome shotgun sequence.
ACCESSION   DASRBV010000217 DASRBV010000000
VERSION     DASRBV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573321"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFA02_08155"
     CDS             complement(<1..549)
                     /locus_tag="VFA02_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZU09863.1"
                     /translation="MIAKAGSFLCADKLPQSQIRRFAWSVLVYNVAVILWGAIVRATG
                     SGAGCGEHWPLCNGTVIQHSPKIETLIELAHRVTSGIALVAILVLLAWTFAITAKRHL
                     ARTAVVAATVLTFNEALLGALLVLLGKVAHDQSASRAVYLSLHLANTLLLLAALALAA
                     HFLSRRSGFMRGSVDFRFLRIAC"
     gene            995..2224
                     /locus_tag="VFA02_08160"
     CDS             995..2224
                     /locus_tag="VFA02_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU09864.1"
                     /translation="MRIFVNEFCGHPFQIQLSRELARRGHEVEHVYFAGNTSTPKGGV
                     HYDYSSYNLNVHGLHITREFTKHSIRSRRAADIEYGEVVALAVHKFKPDVVISANMPL
                     DGQKVLMGAAKRHDAKFIYWLQDVYSMAVRFVLARKLKLLAGIGGAYYEMLEKKLLRE
                     SDAIVCISDGFVDFVKGMGIDGKNIHMIENWSPLDEVMPTPKDNLWAREHGVSDKFCF
                     MYSGTLGMKHRPELLLELAQYLHSTGEAKLIVNAAGAGADWLRENAKDLSPEAFCIQG
                     FQPYERLSEVMGSADVLIALLDSDAGQFAVPSKTLSYLCAGRPILMAAPAMNMAAKVI
                     LRANAGVVVCPDSHDGFVDAAKKLFNDSEQLMTYGENARFYAEQNFNIEHIADRFLGV
                     MKDLKPSDAPLEDPVLA"
     gene            2312..3322
                     /locus_tag="VFA02_08165"
     CDS             2312..3322
                     /locus_tag="VFA02_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU09865.1"
                     /translation="MIIFGGTGFIGTHTALRLLRRPSVEQILLVDIRPPRVAPYTKDL
                     QQALSTGKVRFIQHDIRNPFFADEFAAPDIIFNFAAVHREPGHQPHEYYETNLQGAAN
                     VCAYADAVGCQRIVFTSSISPYGPTEESRHEDSLPVPETPYGGSKLVAEKMHIAWQVA
                     APGRKLLIVRPGVVFGPGEGGNVTRLVRSLVKGYFVYMGNRETRKAGGYVKELVNVFL
                     FGLDALENSDTGVLLLNFSMYPTPALHEYVSAICKAAGFRRSPLSVPRKLLLGISYPI
                     EGVASIFGIRQPISPTRVRKLYRSTNIEARRLQEMGYRYQYTIEEAFADWRNDKPEDF
                     VP"
     gene            complement(3328..4152)
                     /gene="proC"
                     /locus_tag="VFA02_08170"
     CDS             complement(3328..4152)
                     /gene="proC"
                     /locus_tag="VFA02_08170"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZU09866.1"
                     /translation="MNEDLSQIRVAVLGAGKMGGILLQAFLKQQLFTSEQLTATVAHE
                     ERAQVLSAQWGVEVTLDNVAAARNADLILLGVKPFQVGDLIAQIKPALIAEKILISFA
                     TGVKTGTMEEAAGVALPVIRAMPNTPSMLGVGMTALCRGRYVNDEQMALASRIFETIG
                     RTVIVDEKHMDAVTALSASGPAYIYIILESLAEAGVKVGLPRDTATLLAAQMTFGASK
                     MVLDTGYHPALLKDAVTTPAGCTIDGILELEEGGLRVTLIKAVMRAAQRARELATS"
     gene            complement(4246..6024)
                     /locus_tag="VFA02_08175"
     CDS             complement(4246..6024)
                     /locus_tag="VFA02_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZU09867.1"
                     /translation="MKKLLYLILTGVFLAPANLWLHAQGIQSVAERSKALQALFAEIW
                     QNRLQRNPEFASSIGDKRWNDQLTDYSVAAYNQNLERERDYLIRLAAIDTTGLSEQEK
                     LSKDLMARELAQDQEAALFKPWEMPVNQFSGLHSELPRLVSQLSFETVKDYDDYIARL
                     NKVPTAFQQITDNMMSGVEDHRVPPKYLLEKVLTQVNTIAQMKPEDTPFARPLQKIPA
                     GISFADQQRIKTEMLDAIRKQVLPAYTRFAKFLQGVYIPAGRAEPGIWSLPDGDKYYA
                     FLVRRSTTTDLTPDQIHQIGLDEVKRDETEMLAIAQKLGFKDLAALRASIASNPKLHP
                     QSREQLLDLYRHDIDQMRAKLPELFGRLPKAPLVVEPVPAFIEKDQAPAYYEHGTPDG
                     KRPGTVYVNTYDYQHRSLANAESIAYHEGLPGHHLQISIAQELTGIPEFRKYEYYTAY
                     TEGWGLYAERLGKDVGFYQDPYSDYGRLEADIFRAIRLVVDTGVHSRHWTRDQMVQYF
                     HEHSGLDDATVQAEVDRYIAWPAQALGYKMGQLKFLELRAKAQKELGSKFDLRAFHDE
                     VLDSGALPLDVLDEQVTAWIEQQKGK"
     gene            complement(6119..7078)
                     /locus_tag="VFA02_08180"
     CDS             complement(6119..7078)
                     /locus_tag="VFA02_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU09868.1"
                     /translation="MGVLTQWSNGIAVPPPHTESKEIYRLGEMLARSLPMGRLFLRLR
                     QTAAHVRIVTFEGEAGTGKAMAARTLHSLGPASGMAFVPCTAARFVLADVQPLLEEAK
                     GGTLFLTRVGDLAPEQQSRLFDFLEWWEHRNARDVSGFLPRQIFFSSRQPLRQLSANG
                     RMRSDLCYRLAAIRFVLPPLRERREDIAVLAEHFAVTHGAARSKPVRGLHPAALQRLT
                     AHGWPGNVRELETVISNAVMECEGQWIRPIDIPAFASATTQPMLQSVAKAENAAEDDP
                     NLDRMIVRHIRTVLAKTGGNKLRAAQLLGISRSTLYRLLESEA"
     gene            complement(7138..8391)
                     /locus_tag="VFA02_08185"
     CDS             complement(7138..8391)
                     /locus_tag="VFA02_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZU09869.1"
                     /translation="MHEKNNGHSLTTLLGTPVTDVRGQLRGRLKDIAVGTGADAGRVV
                     GLVLKTRDGAKLVPATEVRETPSGVLELRETAELRAVPQDGSFLLLRQDLLDRQIIDV
                     HGRKVVRVNDVDLQWFSHEEKQDLRVREVEVGLRGAVRRLLLGVLPKRALDGLAKKFP
                     PRVIPWEFVDLIEVDPARRVKLKIEHERLAQLHPSDIADILEELAPAEREAIITSLDE
                     EVAADTLEEVDPKLQKALVESLDSEVAAGIVEEMEPEAAADLLAELPEERSEAILEEM
                     APEERQEVEELLEFREDSAAGRMTTAFVKVPFDATVADGVEALRRFEGDPETVTEIYL
                     TDGQDVLKGVIPLARMVLARPETRLSVLAEPRFVSCQADAHQKYVAELFDKYNLRALP
                     VVDDEGRLVGVVEADHVIAFLRESS"
     gene            complement(8519..9274)
                     /locus_tag="VFA02_08190"
     CDS             complement(8519..9274)
                     /locus_tag="VFA02_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09870.1"
                     /translation="MRRFLLSSLLAIGLTGSMFAQQQRQPSDPYAPPPPAPKAPTEQN
                     DKGCSGQATKGPAGGWKTSSTCPPATQNPVLNTPNQDTPKLTTGQSSDKQPKKSTAED
                     NPFPEDVSQKAAEKAREAQQEKQAPPAASEESSSSDKTNGLDLEGEKEGRLSNGAGGI
                     VHNPQLAEHDVHIGEFYLNREDYQGAYDRLKEATLADPANSDAVFYLAEAARHLNHKD
                     EAVQNYQLYLSALPDGPKAKAARKALHELSAKR"
BASE COUNT         2131 a         2779 c         2452 g         2199 t
ORIGIN      
        1 gcaagcaatt cttaaaaagc ggaaatccac actgccacgc atgaatccag aacgccgcga
       61 caaaaaatgc gcagccaatg caagcgccgc caacaacaac agcgtattcg caaggtgcag
      121 cgaaagatac acggcccgcg atgcggattg gtcatgcgca acttttccca gaagcaccag
      181 cagcgcaccg agcagagctt cattgaaggt aagcaccgtt gcagcaacga cagcagtccg
      241 cgcaaggtgt cgtttggctg ttatagcaaa tgtccacgcc agcagaacga gtatggccac
      301 gagcgcgata ccgcttgtaa cacggtgtgc cagctcaatc aacgtctcga tcttcggtga
      361 atgctgaatc acagtgccgt tgcacaacgg ccaatgctct ccgcatccag caccggaacc
      421 tgtggcgcgc acaattgcgc cccatagaat cacagcgaca ttgtagacaa gcacactcca
      481 tgcaaagcgc cttatttggc tctgtggaag cttatctgca catagaaacg aaccagcctt
      541 ggcaatcaca gaatttacct tttttctatt ttacgtgcag cagggaaaac gccgtttata
      601 tgtggcataa aggaatgtag gcgcataaaa ttcacgagat atacaaaatt tcttgtgata
      661 aagtaaaaga gcaagtcaag agttcgcccg gaattatgcg aagaagcaga aatacaatgg
      721 ctccattcat gcttgcaaac ctggtgcttc ttagccactc gtataaagtt acttctaact
      781 tgcgggagtt tcacgcaaaa aaagaaaacc cataagactg cttgtccctc taccagaatc
      841 tgcattgggc ttaacgaata gacctgtttg ctgcatttgt gaaaacgatg tgaatcgggt
      901 cccatggggt tttccccttg ggaacagctg catttcgcac tgtgccgaat tcgcaactct
      961 tactttggcg aaagcctgat aggggtcata tttgatgcgt atttttgtca acgaattttg
     1021 tgggcatcct tttcagatcc agttaagccg cgagcttgcc cggcgcggcc atgaggtcga
     1081 gcatgtctat tttgcaggca atacctcgac gcccaaaggc ggggtccatt acgactattc
     1141 ttcttacaat ctgaacgttc acggcctgca tattacccgc gaattcacaa agcattccat
     1201 tcgcagccgc cgggcagcgg atatcgaata cggcgaagtt gtggcccttg ctgtacataa
     1261 atttaagccc gatgtagtca tctcagccaa catgccgctg gatggccaga aagttttgat
     1321 gggggcagca aaaagacacg acgcaaagtt tatttattgg ctccaggacg tttacagcat
     1381 ggctgtgcgc tttgtgctgg cgcgtaagct caaactgctt gccggtattg gcggagcata
     1441 ttacgagatg ctcgaaaaga agctgcttcg tgaatctgac gctatcgttt gcatctctga
     1501 cggttttgtg gattttgtca aaggcatggg aattgacggc aaaaatatcc acatgatcga
     1561 aaactggtct ccgctggatg aagtcatgcc cacgccaaaa gacaatctct gggcacgtga
     1621 acatggggtc tccgacaaat tctgcttcat gtattcgggc acgcttggca tgaaacaccg
     1681 ccccgagctg ctgctggagc ttgcgcaata tcttcactcc acaggtgagg caaaactcat
     1741 cgtcaacgcg gcaggcgcag gcgccgactg gctgcgggaa aatgcaaaag acctcagccc
     1801 tgaagcattc tgcatccagg gctttcagcc ttacgagcgt ctttctgaag tcatgggctc
     1861 agccgatgtc ctgatcgctt tgctggattc tgacgcagga caatttgcag tcccctcaaa
     1921 gactttgtcc tacttgtgcg cgggccgtcc cattcttatg gccgcaccgg caatgaacat
     1981 ggctgccaaa gtcatcctcc gcgccaatgc aggtgttgtt gtctgcccgg attcccacga
     2041 cgggtttgtg gacgcagcga agaaactttt caacgactct gagcagttga tgacctacgg
     2101 cgagaacgcc aggttctatg ccgaacagaa cttcaatatc gagcatatcg ctgaccggtt
     2161 ccttggtgtg atgaaggacc tgaaacccag cgatgctccg ttggaagatc ccgtcctggc
     2221 ttaatacagc gtaaaatact ggcccgcagg aggcccctgg acccatcagg gcttcctgtg
     2281 tccattagag gaaatttcta tcttgaagcg agtgattatt tttggcggca caggctttat
     2341 tggaacgcac acagctctgc gtcttctgcg caggccatct gtagaacaaa tcctgcttgt
     2401 agacatccgt ccaccgcgcg tggcccctta tacgaaggac ctgcagcagg cattgtccac
     2461 cggaaaagtc cgttttattc agcatgatat tcgcaatcca ttttttgcgg acgaatttgc
     2521 cgcacccgat atcatcttta acttcgcagc ggtacaccgc gagcctggtc atcagccgca
     2581 tgaatactac gaaacaaatc tccaaggcgc ggccaatgta tgtgcctatg cagatgctgt
     2641 aggctgccag cgtattgttt tcaccagctc gatttctccc tatggtccta cggaagaaag
     2701 caggcacgag gattcgttgc cagtaccgga aaccccgtac ggcggttcaa aactggttgc
     2761 cgaaaaaatg cacattgcat ggcaggtggc tgcgccggga agaaagctac tgatcgtgcg
     2821 ccccggcgta gtcttcggcc cgggagaagg cggaaatgtt acgcgcctgg tgcgcagcct
     2881 ggtcaagggg tatttcgtct acatgggcaa ccgcgagacc agaaaggccg gagggtatgt
     2941 caaggagctc gtgaacgttt tcctctttgg cctggatgct cttgaaaaca gcgataccgg
     3001 cgtactgttg ctgaatttct ccatgtaccc tacgcctgcc cttcatgagt acgtcagcgc
     3061 catctgcaag gctgccggat tccgccgcag cccgctgtcg gtgccgcgca agttgttgct
     3121 tggtatttct tatccgatag aaggcgtcgc gagcatcttt gggatcaggc agcccatctc
     3181 accgacccgc gtccgtaagc tgtatcgctc caccaatatt gaggcgcgac gcttacagga
     3241 gatgggctat cgctatcaat acaccatcga agaagcattt gccgactggc gcaacgataa
     3301 acccgaggac tttgttccgt agcggaatta acttgtagcc agttccctcg cccgctgggc
     3361 cgcccgcatc acggctttta tcagcgtgac tcgcaggccg ccttcttcca gttccaggat
     3421 gccgtctatg gtgcatccag cgggcgtggt tactgcatct ttcagcagcg ccggatggta
     3481 gccggtatcg agcaccattt tggacgcacc aaaggtcatc tgcgctgcca gcagcgtggc
     3541 tgtatcacgt ggcaggccta cttttactcc tgcttccgcc agcgattcaa gaatgatgta
     3601 aatataggca gggccgcttg ctgagagcgc agtgactgca tccatgtgtt tttcatctac
     3661 gataacagta cggccaatcg tttcaaaaat gcgcgaagcg agcgccatct gctcgtcgtt
     3721 tacataacgt ccgcggcaga gcgccgtcat gcctacgccc agcatggaag gcgtattcgg
     3781 catcgcgcgg atcacaggga gcgccactcc ggcagcctct tccatcgttc ccgtctttac
     3841 cccggttgca aaggagataa ggatcttctc cgctataagc gcaggcttga tctgagcgat
     3901 caagtcgccc acctgaaacg gctttacgcc taacaaaatc agatcagcat ttcgtgccgc
     3961 agcaacattg tccagtgtta cttcaacacc ccattgcgcg gaaagtacct gcgcgcgctc
     4021 ctcatgcgcg acggtggccg taagctgctc cgaagtaaag agctgctgct tcagaaatgc
     4081 ctgaagcaga atgccgccca tttttcctgc tcccaggacg gcaacacgga tctgcgaaag
     4141 gtcttcgttc atctttccct cattggaatg ggtgcctcgc atctcaaaag aaacgggagt
     4201 tgcactatat cggaggccct caaaacttca gcctccttct cgctcttatt tccctttttg
     4261 ctgctcaatc catgctgtta cctgctcgtc cagcacatcg agcggcagcg cgcctgaatc
     4321 gagcacttca tcatgaaagg cgcgaagatc aaatttgctg cccagctcct tctgggcctt
     4381 tgctcgcagc tccagaaact ttagctggcc catcttatag ccaagcgcct gtgcaggcca
     4441 tgcaatgtag cggtctactt cagcttggac ggtcgcgtca tccagtccgg aatgctcatg
     4501 aaaatactgc accatctggt cgcgggtcca gtgtctggag tggacgcccg tatccacaac
     4561 caaccgaatg gcgcggaaaa tatcggcctc aagccgtccg taatcagaat aggggtcctg
     4621 ataaaagcct acatctttgc ccaaccgttc cgcatacagt ccccagcctt ctgtataggc
     4681 ggtgtagtat tcgtatttcc ggaactcagg aataccagtc agttcctgcg caattgagat
     4741 ttgcaggtgg tgcccgggaa gtccttcgtg gtacgcaatg gattccgcat tcgccaggga
     4801 acggtgctgg taatcatatg tgtttacata caccgtcccc ggacgcttgc catcgggcgt
     4861 tccgtgctca taataggcag gcgcctggtc tttttcgata aaggccggca caggctcaac
     4921 caccagcggg gccttcggta aacgtccgaa gagttcaggc agttttgccc tcatctggtc
     4981 aatatcgtgc ctgtataaat cgagcagttg ctccctgctc tgcggatgca gcttgggatt
     5041 gctcgcaatc gaagcacgca gcgcagcaag gtctttgaag ccgagctttt gcgcgatcgc
     5101 cagcatctcg gtctcatcgc gcttcacttc atccagacca atctggtgaa tctggtcggg
     5161 agtcaggtcg gtagtcgtgc tgcggcgcac aagaaaggca tagtatttgt cgccgtccgg
     5221 taacgaccag atgcccggct ccgcccgccc tgccggaatg tagacacctt gcagaaactt
     5281 tgcaaatcgc gtgtaggctg gcagcacctg cttccggatc gcatccagca tttcagtctt
     5341 gatgcgctgc tggtcggcaa aggatatgcc tgccggaatc ttctgtaatg gacgcgcaaa
     5401 cggagtgtct tccggcttca tctgcgcaat ggtgttcacc tgcgtcagta ctttttccag
     5461 cagatacttt ggcggaacac gatggtcttc cacgccactc atcatgttgt ccgtaatctg
     5521 ctgaaatgcc gtagggactt tgttcaaccg agcaatgtag tcgtcatagt ccttgacggt
     5581 ttcaaaactc agttgcgaaa ccagccgggg cagttcagaa tgcaggccgc tgaactggtt
     5641 gacaggcatc tcccacggtt tgaaaagggc cgcctcctgg tcctgggcca gttcgcgggc
     5701 catcaggtct ttgctcagct tttcctgctc gctcagaccg gtggtgtcaa tcgcggccag
     5761 ccggatcaga tagtcgcgct cgcgctccag gttctggtta taggcggcaa cggagtagtc
     5821 agtaagctgg tcgttccagc gcttgtcgcc tatggaggaa gcaaactccg gattgcgctg
     5881 tagccggttt tgccagattt cagcaaaaag ggcctgcagg gctttgctgc gctccgccac
     5941 gctttgtata ccctgggcat gcagccagag atttgctgga gctaggaaaa ccccggtcag
     6001 gatgagataa agaagctttt tcatggctta gaaggcagtg tatcaaggag gagccttcca
     6061 gccgaagatt tccggtgtcg ggagtgtgct cacatcgggc gggaattcag ttttatcttc
     6121 acgcttccga ttccaacagc ctgtaaaggg tagagcggct gatacccaac agttgagcag
     6181 cgcgcaactt gtttcccccg gtttttgcca gaacggtcct gatatgccgc acaatcatcc
     6241 ggtcaaggtt tggatcatcc tcagcagcat tttctgcctt tgccacggac tggagcattg
     6301 gctgcgtggt tgcagacgca aacgctggaa tatctatggg gcgtatccac tgcccttcgc
     6361 actccattac cgcattggaa atgacagtct caagttcgcg aacatttccc ggccagccat
     6421 gcgctgtcag ccgctgcaat gcagccggat gaagtccacg caccggtttg cttcgcgcag
     6481 caccatgcgt aacggcaaag tgctccgcca gcacagcaat gtcttcacgc cgctcgcgca
     6541 aaggaggcaa aacaaagcgt atcgcagcca gccggtaaca aagatcagag cgcatcctgc
     6601 cattggcaga gagctgacgc agaggctgtc gcgaactaaa gaatatctgc cgcggcaaaa
     6661 acccgctgac gtcccgcgca ttgcggtgtt cccaccattc cagaaaatca aaaagacggg
     6721 actgctgctc cggcgcaagg tcgcctactc gcgtcaggaa aagcgtgccg cctttggcct
     6781 cctctaacag aggctgtaca tcggccagca caaagcgcgc agccgtacat ggaacaaagg
     6841 ccatgccaga tgcaggccca agcgaatgaa gcgtccgcgc agccatcgcc ttgcctgttc
     6901 ccgcttctcc ttcaaatgta acaatgcgca cgtgtgcggc cgtctgccgc aggcgcaaaa
     6961 aaaggcgccc catgggcaga ctgcgcgcca gcatttcgcc caaccggtaa atttccttgc
     7021 tttctgtgtg cggcggcgga acggctatac cgttgctcca ttgcgtcaat acacccacag
     7081 cagttactcc gtgaagaatt tgtcttgtgg agtgtatcgg caattctgtg caatggatta
     7141 actgctttcc cgcaggaaag caatcacgtg gtctgcttcc accaccccga ccagccgccc
     7201 ctcatcgtcc accactggaa gcgcacgcag attgtattta tcgaatagct cggccacata
     7261 tttctggtgc gcgtccgcct gacaggagac aaaccgcggc tcggccaaca cgctcaacct
     7321 tgtttcaggc ctggccagca ccatccttgc caatggaatg acccctttca ggacatcctg
     7381 cccgtctgtg agataaatct ccgtcaccgt ctcaggatca ccttcaaacc gacgcagggc
     7441 ttccactccg tctgctactg tcgcatcaaa tggcaccttc acaaacgccg tcgtcatacg
     7501 gccggcagca gagtcttcgc ggaattccag cagctcttcc acttcctgcc gctcttccgg
     7561 ggccatctct tccagaatgg cctctgaccg ctcttccgga agctccgcca gcaggtcagc
     7621 cgcggcctcc ggttccatct cttccacaat gcctgcggcg acctcagaat ccaatgactc
     7681 aaccagcgcc ttttgcagct ttggatcaac ctcttccagc gtatctgcgg caacctcttc
     7741 atcgagtgac gtaatgatgg cttcgcgctc tgccggggcc agctcttcca gaatgtcggc
     7801 aatgtcggat ggatgaagtt gcgccagacg ctcgtgctca atcttcagct tgacccggcg
     7861 ggccggatcc acctcaatca aatccacaaa ctcccaggga atcactcgcg gcggaaactt
     7921 cttcgccagc ccgtccaatg cacgctttgg caagaccccc agcaaaaggc ggcggaccgc
     7981 gccgcgtaaa ccaacttcta cttcgcgcac ccgcaggtcc tgcttttctt catgcgagaa
     8041 ccattgcagg tccacatcat ttacgcgcac aaccttacgt ccgtggacgt caataatctg
     8101 acggtcgagc aggtcctgcc gtagcagaag aaaactcccg tcctgcggca cagcgcgcag
     8161 ctctgctgtc tctcgcaact caaggacccc gctgggcgtc tcccgcacct cggtcgcagg
     8221 aacgagtttt gccccatcgc gcgtcttaag caccaggcca accacacgcc ccgcatccgc
     8281 gcctgtgcct acagcaatgt ccttgagccg gccacggagc tgcccgcgca catccgttac
     8341 cggagtacca agcaaagtgg tcaggctgtg cccattgttc ttctcgtgca taggcttcct
     8401 cgtgcagtct atagcaaaac aaagcgagtt ccctcgccgg acagcaacaa acagccagca
     8461 attccattgg atggttactt ttcaacggaa ggaatgcgta cagatgggaa ttgacagttc
     8521 accgctttgc agaaagctcg tgcaatgcct tccgcgcggc cttcgctttc ggcccgtccg
     8581 gtaacgctga cagataaagc tgataattct gcaccgcttc gtctttatgg tttaaatgcc
     8641 gcgctgcctc ggcgagataa aacactgcat ctgaattggc gggatcagcc agggtcgctt
     8701 ctttcagccg gtcgtaggcg ccctgataat cttcgcgatt caggtagaac tcgcctatgt
     8761 gaacatcgtg ctcggcaagc tgcggattat gcacaattcc tccggcgcca tttgagagcc
     8821 ggccttcctt ctcgccttcc agatcaagcc catttgtctt gtcactactg gagctttcct
     8881 ccgaagctgc aggcggtgcc tgcttctcct gctgtgcctc tcttgctttt tccgcggcct
     8941 tttgcgagac atcctcagga aatggattgt cctccgccgt ggatttcttc ggctgcttat
     9001 cagagctctg cccggtcgtc agctttggcg tgtcctggtt tggcgtgttc aggaccggat
     9061 tctgcgttgc aggcgggcag gtgctgcttg tcttccatcc tccggccggg cctttcgtgg
     9121 cctgcccact gcatcctttg tcgttttgtt ccgtgggcgc tttgggcgct ggcggaggcg
     9181 gcgcataggg atcagacggc tgcctttgct gctgtgcgaa catgctcccg gtcagtccaa
     9241 tggccaataa cgaactcaaa agaaaacgcc gcatactcta ttaagagtag acgcggcggc
     9301 cacgcgaaag gatgatacaa attcgccgcg cttccatctg gcgcgcgcaa cattccggaa
     9361 acctgggcct gcggcgtcat gctgaaatca gctatactcc cctcaaatat gggccttatt
     9421 atccgttctt ccgccatcca tgcagcaggt tgctacacca ccacgccaat caaaaaaggc
     9481 gcgcgcgtgg ttgaatatac cgggcctcgc atcaccaaag acgaggctga tgagaagtat
     9541 cagaactcgc cgaccacata c
//
LOCUS       DASRBV010000218         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_114002, whole genome shotgun sequence.
ACCESSION   DASRBV010000218 DASRBV010000000
VERSION     DASRBV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114002"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFA02_08195"
     CDS             complement(<1..185)
                     /locus_tag="VFA02_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09871.1"
                     /translation="MANRKEWTEWHLTPRGWERGATRVQGQGNTWVEEPTDRVLSFVY
                     QELETDSSPEVKKWAEET"
     gene            245..901
                     /locus_tag="VFA02_08200"
     CDS             245..901
                     /locus_tag="VFA02_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU09872.1"
                     /translation="MVLETFPVGLLQCNCTILGDETTGKAMVVDPGDNLPEILARLNK
                     HGLKLKQIVVTHAHIDHVGGALRLKQQTGAPILLNQNDLPLLDMMEMQAGWLGVSTPA
                     VAPPDASADDHLVIGLENYPAQVLHTPGHTPGSICLHFAPENLLIAGDTLFAGSIGRT
                     DLPGGDFRKIIHSIHDRLLALPDETRVIPGHGPSTTIGEERESNPFLAAPFRNSFFIA
                     "
     gene            980..2161
                     /locus_tag="VFA02_08205"
     CDS             980..2161
                     /locus_tag="VFA02_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZU09873.1"
                     /translation="MLAAAVVLLLPFAGCNKPAPPAQNETPGKPAVAQIGGEDVVKLQ
                     RTATSNGAKPEFLSATILPGRGMNLFQITANIPGKGEVNVLASPSLDEAAQKLNGGPA
                     DLNGNASFSFGGAFLVPYPNRIRGKLSADGKTITTEWNGKQITLPANWAGKKPGAEKH
                     AMHGLILASKTDDVHVENTSDGQTVTGVIHAGNFGGYWPSQTDLNFTISLAGDAVEAT
                     ITAKNVGNEPEPISIGWHPYFSVPSGNRTQARLHVPGEVVAQVNNYDDVFPTGKLLPV
                     KGTKYDFTAPDGKPLDDIFLDDNFAHLKRTGGNVVVDFTDPASQYGIHIIGESPEIKT
                     VQVYAPPDKQFAAIEEQFNFGDPWGKEWHGMDTGMVTLKPGQSVTWKCKLSLFTPPAA
                     K"
     gene            complement(2238..2741)
                     /locus_tag="VFA02_08210"
     CDS             complement(2238..2741)
                     /locus_tag="VFA02_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU09874.1"
                     /translation="MKLAELFLAQLENEAASSRRVLERVPDGRNDWKPHPKSMPLGYL
                     AALVASMPSWIEMMIASDEFDITDETSEYKNREVQSTRELVALLEQSINKARVALQNT
                     TDEHLLKPWKLIAHGFLASENPRYVMIRDAVFSHLAHHRGQLTVYLRLNEAAVPGIYG
                     PSADEGF"
     gene            2832..>3756
                     /locus_tag="VFA02_08215"
     CDS             2832..>3756
                     /locus_tag="VFA02_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZU09875.1"
                     /translation="MRADRLMSALLLLQARGQMTGRELADKLEVSERTIHRDMEALGA
                     AGIPVYTLRGQQGGWRLDEDWRTQVPGLDEAELRALLMAQPRSLGDKHLAAAADRAIG
                     KLMAALPKPLQEQASSIRDRLYIDTTGWTGTSENLSMLSVVQTAVASDRKLHFWYWRA
                     GRERVERTVDPLGLIAKGSTWYLFAKTERGHRTYRISRMEEAKLLDEPSQRPANFNLE
                     TAWKLSTEEFRDEVNRELEASRRAYEARKEAERRAAQELEIAKQVQSRLFPQCQPVCP
                     TLDYAGICIQAREVGGDYYDFLDFGRQKIGFV"
BASE COUNT          841 a         1049 c         1053 g          813 t
ORIGIN      
        1 gtttcttcgg cccacttctt tacttccggc gagctatcgg tttccaactc ctggtaaaca
       61 aagctcaata cgcggtcggt tggttcttca acccaggtat tgccctgtcc ctgcacccgt
      121 gttgcgccac gttcccagcc acgcggagtc aagtgccatt ccgtccattc cttgcgattt
      181 gccatagcga aatattgtga cgcattttgc gcaatccggg tagcttcgca gacaatcaaa
      241 attgatggtt ctggaaactt ttcctgtcgg attgctccag tgcaactgca ccattctggg
      301 cgatgaaacc acgggcaaag ccatggtggt ggatccgggc gacaatcttc cggagattct
      361 ggcccgattg aacaagcatg gtctgaaact gaagcagatc gtcgtcactc atgcgcatat
      421 tgaccatgta ggcggcgcgc tcagactgaa acaacagact ggagcgccca ttctgcttaa
      481 ccagaacgat cttcctttgc tcgacatgat ggaaatgcag gccggatggc ttggtgtctc
      541 tacgcccgcg gtcgccccgc ccgatgccag cgcggacgac catcttgtga ttgggctcga
      601 aaactatcct gcgcaggttt tgcatacccc ggggcacact ccgggaagca tctgcctgca
      661 ttttgcccca gaaaacctgc ttattgcggg cgacacactg tttgccggct cgattggccg
      721 caccgacctt cccggaggtg acttccggaa gatcatccac tccattcatg accggctgct
      781 cgcgttgccc gatgagacac gcgtgattcc gggccatggc ccctctacaa ccattggcga
      841 agagcgggag tcaaacccgt tcctcgccgc cccgttccgg aacagcttct tcatcgcgta
      901 gaatgggaac cgcttcggca aacgtttcct atccctacct atgaggtttc gatccatgtc
      961 cgtttgtgag aaacgacact tgcttgcggc agcggtggtg ctgctgcttc catttgcagg
     1021 atgcaacaag cctgctcctc ccgcgcagaa tgagactccc ggcaaacctg cggtcgcgca
     1081 gattggcggc gaggacgttg tgaagttgca gcgcacggcc accagcaatg gagcgaaacc
     1141 ggagttcctc tctgcgacca ttctgcccgg gcgcggcatg aacctcttcc agattacggc
     1201 caacattccc ggcaagggag aagtcaacgt ccttgcatcg ccttctctgg atgaagcggc
     1261 gcagaagctg aacggcgggc cagcggacct gaacggaaac gccagcttct cttttggcgg
     1321 agcatttctg gtcccatatc cgaaccgcat tcgcggcaag ctctcggcag acggcaagac
     1381 gatcaccacg gaatggaacg gcaaacagat tactctgccg gcgaactggg caggcaagaa
     1441 gccgggcgcg gagaagcacg ccatgcacgg gctgattctt gcaagtaaga cagatgatgt
     1501 tcacgtggaa aatacttcag acggacagac ggtgaccggg gtcatccacg caggcaattt
     1561 tggcggatac tggccttcgc agaccgacct gaactttacg atctcgcttg ccggcgatgc
     1621 ggttgaagcg acgattacgg caaagaatgt tggcaatgag ccggagccaa tctctatcgg
     1681 ttggcaccca tacttctctg ttcccagcgg gaaccgtaca caggcccgcc tgcacgttcc
     1741 gggagaagtg gtcgcgcagg tcaacaacta tgacgatgtt tttcctacgg gaaagctgct
     1801 tcctgtaaag ggaacgaagt atgacttcac cgctcccgat ggcaagccgc tggatgacat
     1861 ctttctcgat gataactttg cgcatctgaa gcggacaggc ggaaacgtgg ttgtggactt
     1921 taccgatccg gcatcgcaat acggaatcca catcatcggc gagtcaccag agatcaagac
     1981 tgtgcaggtg tatgctccgc cggacaaaca atttgccgcg attgaagagc agttcaattt
     2041 tggcgacccg tggggcaagg agtggcacgg catggacacg ggaatggtga cgctgaagcc
     2101 agggcagtcg gtaacatgga agtgcaagtt gtcactgttt acgcctccgg cagcgaagta
     2161 aagccgtctg gaattaccca ttccatacac aggattggcg catggaatgg gtggttcctt
     2221 tcttctgtct gattgcctca aaatccttca tctgctgaag gcccgtaaat gccagggacc
     2281 gcggcctcat tcaaccgaag atagactgta agctggccgc gatggtgtgc cagatggctg
     2341 aagacagcat cgcgaatcat cacgtagcga gggttttcac tggccagaaa gccgtgagct
     2401 atcaacttcc agggcttcag cagatgctcg tctgtggtat tttgcaaagc cacgcgtgcc
     2461 ttgttgatgg attgttccag cagcgccacc aattcgcgtg tgctctgcac ctctctgttc
     2521 ttgtattctg atgtctcatc ggtaatgtcg aactcatctg atgcaatcat catttcgatc
     2581 catgaaggca tagacgctac cagggcagca agatagccca gcggcatgga tttcggatgc
     2641 ggcttccagt cattgcgtcc atctggcaca cgctccagca cccgcctgct tgacgccgcc
     2701 tcattctcta attgcgcaag aaacaattcc gccagcttca taaaaacatc ctccttgact
     2761 acgcgaacca gactaaccgc tcaaatatga catcttgtgt cataattggc gggaattctg
     2821 aaaatttttt tatgcgagct gaccgtctta tgtccgcgct gttgctgttg caggcccgag
     2881 gccagatgac cgggcgcgag ctggcggaca agctggaagt ttcagaacgc acgatacatc
     2941 gcgatatgga ggccctggga gcagcaggca ttcccgtgta tacgctgcga ggccagcagg
     3001 gcggatggcg gcttgacgaa gactggcgca cgcaggttcc agggctggat gaagccgagc
     3061 tgcgcgcgct tctgatggca cagccgcgtt ctttgggaga caagcatctg gccgcggcgg
     3121 ctgatcgagc gataggaaaa cttatggccg ccttaccgaa acctctccaa gagcaggcat
     3181 cttctattcg cgaccggctc tatattgaca ccacaggctg gacgggaacg agcgaaaatc
     3241 tctcgatgct ttccgttgtg cagacggctg tggcaagcga ccgtaagctg cacttctggt
     3301 actggcgtgc agggcgggag cgcgtggaac gaacggtgga cccgctgggg ctgattgcaa
     3361 aaggcagcac atggtatctg tttgcgaaaa cagagcgcgg gcaccgcaca taccgcatat
     3421 cgcggatgga agaagcgaag ctgctggacg agcccagcca gcgtccagcc aattttaatc
     3481 tggaaactgc ctggaagctt tcaacagaag agtttcgcga tgaagtgaac cgggaactgg
     3541 aagcctcccg gcgtgcctat gaagcccgga aagaggcaga acgccgcgct gctcaggagc
     3601 tggaaattgc aaaacaggtg cagtcacgcc tctttccgca atgtcagccg gtctgtccga
     3661 ccctcgatta tgccggcatc tgcattcagg cgcgcgaggt cggcggagat tattacgatt
     3721 ttctcgactt tggcaggcag aagatcggct ttgttg
//
LOCUS       DASRBV010000219         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_727401, whole genome shotgun sequence.
ACCESSION   DASRBV010000219 DASRBV010000000
VERSION     DASRBV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727401"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VFA02_08220"
     CDS             <1..2044
                     /locus_tag="VFA02_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZU09876.1"
                     /translation="PVVEYDKRMIRSSVQFALLALGLAFAQGSAAQENLTSPVIIVVS
                     PDGNDAADGTSDHPFRTLQRAQEAVRLANEMHDVEVRLGDGMYRLTGTLRFSARDGGR
                     NGHHVVWMAAPGAHPVISGAIRVTGWKLFDKARQIYVANTPAGLDSRQLWVNDHLAQM
                     AEIEIPRADVAFTRDGIVLKDPKYEYQSKLPEQDRIEVRATGFFTMRISRVEKISGLN
                     LVMMQPAWDNNLWGYDTIEAPFHPELAHLYLANSLAFLSKPGQWYLDAAAGKLYLRPP
                     DDADVQKMDVELPRLPVLISIGDSLDAPVEDLTFRGIRFSHTSWLGPSSEEGYASQQS
                     GSYLAGHAALYPADPIASCARGCRAFESVRNEWSQMPASIQVSAAQRIVFDHCIFAHL
                     GQYALGIGNDADAMLSGTGLGTSDITIASNEFTDDAGGAILAGGVRRDAHHPRDPRMI
                     NRQIIIRDNLIHSVSEDYQDNSAILSTYVAGAIILHNEISGVPYDAIDIGYGWGMQDP
                     GGNPNYRVYMHGYDFPQNKVYDTPTTHHDVVVAGNRIHDAKKLFQDGGAIYNLSASPG
                     TLITENYIYDNHGRIALYLDEGSRYITVRRNVVQDPEGEWLNINTVRHAYPLRISTDN
                     TATDNWHDSSKTGGMWTNYQNNLILNDHLIQNGNWPAEAKQIMRYAGTEEDARPAK"
     gene            complement(2050..3255)
                     /locus_tag="VFA02_08225"
     CDS             complement(2050..3255)
                     /locus_tag="VFA02_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU09877.1"
                     /translation="MVIPLSAQVSSRQIDTWRKEIRRALFVPDPLPALEVKTWSSFSP
                     VPGVVADRVTYATEYGMLVPAIVYHPAKFNGKAPGIVVVNGHGADKTSWYSWYTGILY
                     AKAGAVVVTYDPVGEGERNDDHKDGTGEHDTVISVPGVAQRMGGLMQTDVMQAVSYLR
                     SLPETDKRRIAILGFSMGSFVSAITGAIDPRIHAVLLTGGGDLDGPGGYWDSSHMVMC
                     QSGPWHALEFLGDRPAVIYTLNARRGPTFIMNGTNDTVVAIPQHGQQFFDDLRKRVIA
                     MNGSEKNVFQTWFDPGASHRPAWVLKTAAEWLNSVLHFPGWTPEQIAKLPIVRIGDWA
                     AANGVHLSKSDMRQDRAAGLEAIDVGVPRLVPQLLSVLPMNEWQKEKDRFAYSAWVRK
                     AIADAEAKH"
     gene            3420..4676
                     /locus_tag="VFA02_08230"
     CDS             3420..4676
                     /locus_tag="VFA02_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HZU09878.1"
                     /translation="MKTRRRTALCFCAAMFGAVSLLNAATVCNPATYGAKADGVTKDT
                     QAIQKAIDVCAEKGGGIVRLQKGTFLTGPIQLKSHITLEVQTGATLLGSQDRNDYKEA
                     EELRETSVEPLIGAKNAEDITIRGGGTIDGNGKPWWDDVYSHRHVPDFVAAKRPRLIL
                     LDHCKHVLIENVTIQNSASWQVVPYYCDDVIIRNGKILAPAHSPNTDGIDPFSSHHVT
                     ISHMTIDVGDDNIAIKSGQPGSPGPDDPSTDITVTDCNFLHGHGMSIGSEIAGGVQNV
                     HVARVHFDGTGNGVRIKSNRDRGADIGNFEFRDLTMENVGTAILISMYYPKIPQNDTA
                     QPVTHLTPRFHDIHIENVRATGAKEAGVIIGLPEMPITTITLDNVQLSAQKGMTISNA
                     TVTAHNFHVQAAEGQPVILLNNAKLQ"
     gene            4673..>6172
                     /locus_tag="VFA02_08235"
     CDS             4673..>6172
                     /locus_tag="VFA02_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HZU09879.1"
                     /translation="MRRRVFAKNFLLLVVCAGASYAQTTVKACAVLKATHTSSDEAEA
                     ADTARIQSALSQCSPGKAVVLQASGSKDAFISAPLLLPRGVTLFISRGVTLFASRNLR
                     DYDLHPGSCGKAGCKPFLYAYQAAWSGVAGEGTIDGQADKTAWWRIREESKTAQHQGN
                     PQGVVPDLIASYESQNFSVYGLHLKNTAGDSVAAYKTIGFKADDLVMDAPHGGDGILL
                     SNSPDASLRNLNIRVDGAAIDIRASILGGTSRVNISGLHVNGGEGIAFGDSQYGDVHA
                     VHISNSTLGNAGLKFDLRGVRGGILRDVQFDNVCLAGSVTPLTVQSQSGTASVLPTDQ
                     SIRFANVVVRGRGLLTAHGVQEILNAVCPETDQTPTAISWVVNTSAIHHPGTRSKLVV
                     AEDGSGDFRTVQDAVDALPETGGDIAVKPGVYREVVTIRKPHVHLYGENSDAQKTEIV
                     FNNTGPHNGGTFNSATVFVEADSDSISNLTIANDAGSGKGQAVALAVTGD"
BASE COUNT         1456 a         1641 c         1782 g         1293 t
ORIGIN      
        1 gcccgttgtt gagtacgaca agagaatgat acgcagcagc gtacagttcg cattattggc
       61 cctagggctt gccttcgcgc aaggaagtgc cgcccaggag aatcttacgt ccccggtcat
      121 cattgttgtc tcgcctgacg ggaacgatgc tgctgatggc acatcagacc atccgttccg
      181 gacgttgcaa cgcgcgcagg aagccgtgcg gcttgcaaat gaaatgcatg atgttgaagt
      241 gcgattgggc gatggaatgt accggctgac gggcacacta cgtttttccg ccagagacgg
      301 cggtcgcaat ggacatcatg ttgtgtggat ggccgcgccc ggagcgcacc cggtcatctc
      361 cggagccatt cgcgtgactg ggtggaagct ctttgataaa gcaaggcaga tttatgtagc
      421 caacacacct gcaggactgg acagccgtca gttatgggtc aatgaccatc tggcacagat
      481 ggcggagata gagattccgc gcgcggacgt tgcgtttacg cgagatggaa ttgtcctaaa
      541 agacccgaaa tatgaatacc agagcaaact gccggaacaa gaccgcatag aggtgagggc
      601 gacaggattt ttcaccatga ggatttctcg tgttgagaag atcagcggcc tgaatctggt
      661 gatgatgcag cctgcgtggg acaacaattt gtgggggtat gacacgattg aagcgccatt
      721 tcatccggag ctggcacacc tgtatcttgc aaattcgctg gcgttcctaa gcaaacccgg
      781 gcagtggtat ctcgatgccg cagcaggaaa gttatatctg cgtccgccgg atgacgcaga
      841 cgtgcagaag atggatgtgg aactgccgcg attgcccgtg ctgatttcga ttggcgacag
      901 tctggatgct ccggtcgagg accttacatt tcgcggtatc cgcttctcgc acacaagctg
      961 gctggggcct tcgagtgagg aaggttatgc aagccagcag agcggttcgt atcttgcagg
     1021 acacgcagct ctctatcccg cagatccgat tgcaagctgc gcgcgcggct gccgcgcgtt
     1081 tgagagtgtg cgcaatgaat ggagccagat gccggcctct atccaggtct cggcagcgca
     1141 gcggattgtg tttgaccact gcatctttgc gcatcttgga caatatgcgc tgggcatcgg
     1201 caatgatgcg gatgcgatgc tctccggaac gggcctggga acaagcgaca tcacgattgc
     1261 ttccaatgaa tttacagacg atgcaggtgg ggccattctg gccggaggag tccggcgcga
     1321 tgcgcaccat ccgcgcgacc cgcgcatgat caaccggcag atcatcattc gcgacaacct
     1381 cattcattcc gtcagcgaag actatcagga caacagcgcc atcctaagca cgtatgttgc
     1441 cggtgcaatc attctgcaca acgagatttc aggcgttccc tatgatgcga ttgatatcgg
     1501 ttatggctgg ggcatgcagg acccgggcgg aaacccgaac tatcgcgttt acatgcacgg
     1561 ctatgatttt ccgcagaaca aagtgtatga cactcccacg acgcatcacg acgtcgtcgt
     1621 ggcaggcaat cgcatccatg acgcaaagaa gctgtttcaa gatggcggcg caatttataa
     1681 cctttcggcg agtccgggaa cactgataac cgagaactat atctatgaca atcacggcag
     1741 gattgctctg tatctggatg agggatcgcg ctatatcacg gtgcgcagaa atgtggtgca
     1801 ggacccggaa ggagagtggc tcaacatcaa tacggtgcgc catgcgtatc ctctgcggat
     1861 ttcaaccgac aataccgcga cggacaactg gcatgacagc agcaaaacag gcggaatgtg
     1921 gacgaattac cagaacaatc tgattctgaa tgaccatctg attcaaaacg gcaactggcc
     1981 agccgaagca aaacaaatca tgcggtatgc cggcacggaa gaagacgcga ggccagcgaa
     2041 gtgatgacct tagtgttttg cctccgcatc agcgatcgct ttgcgcaccc aggccgagta
     2101 tgcgaagcgg tctttttcct tttgccattc attcatcgga agaacgctga gcagttgcgg
     2161 aaccagtctg ggcacaccaa cgtcaatggc ttccagtccg gcggcacggt cttgtcgcat
     2221 gtcgctcttg ctgagatgca cgccgttcgc ggcggcccag tctccgatgc gcacaatggg
     2281 aagttttgct atttgctcag gtgtccagcc gggaaaatgg agcacgctgt tgagccattc
     2341 tgcagccgtt ttcagcaccc atgcaggacg atggctggcg cctggatcga accatgtctg
     2401 aaatacattt ttctccgaac cgttcatcgc aatcacacgt ttgcgcaggt cgtcgaagaa
     2461 ctgctgaccg tgctgaggga tggccactac agtatcgttt gtgccattca tgatgaaggt
     2521 cgggccgcgg cgcgcattca gggtgtagat gacggctggc cggtcaccga gaaactcaag
     2581 cgcgtgccac ggaccagact ggcacatgac catgtggctc gaatcccagt agccgccggg
     2641 accgtcgagg tcgcctccac cagtgagcag cacggcgtga atgcgtgggt caatggcccc
     2701 ggttatcgcg ctcacaaacg agcccatgga gaagccgaga atagcgatgc ggcgcttgtc
     2761 agtttccggc aggctgcgca ggtaagagac agcctgcatt acgtcggtct gcatcagtcc
     2821 gcccatgcgc tgcgcgacgc cagggacaga gatgaccgtg tcgtgctcgc ctgttccatc
     2881 tttgtggtcg tcgttacgtt cgccttcacc aaccggatca taggtgacga ccactgctcc
     2941 ggcttttgca taaaggatgc cagtgtacca ggagtaccag cttgttttgt ccgcgccgtg
     3001 tccattgact acaacaatgc cgggggcctt gccgttaaac tttgcagggt ggtagacgat
     3061 ggcaggaaca agcatcccgt attccgttgc ataggtgacg cggtcggcaa ctacgcccgg
     3121 aacgggagaa aagctgctcc aggtttttac ttcaagagca ggaagcgggt caggaacaaa
     3181 caacgcacgg cgaatttcct tacgccatgt atcaatttgt ctggaagaaa cctgcgcgga
     3241 tagaggtatg accacaagaa agatcaaact aaccgacacc atgactgatt tgcatttaaa
     3301 aaagtggctt cgcaacggct tttcctgaag aagaggatga cgtgcctatg atattctcgc
     3361 taaccagaga ctgcaaaggt ctgacctctc acaatcttgc tgatgtggag aaaattcaga
     3421 tgaaaaccag aagaaggacg gccctgtgtt tctgcgccgc aatgtttgga gctgtgtccc
     3481 tgctcaatgc ggccacagta tgcaatccgg cgacatacgg cgccaaagcc gatggggtca
     3541 ccaaagatac ccaagccatc cagaaggcga tagatgtttg cgcagagaaa ggcggaggca
     3601 ttgtccgcct gcaaaaaggg acatttctca ccgggccgat tcaactgaag agccacatca
     3661 cgctggaggt ccagacaggc gcaacgttgc tgggcagcca ggaccgcaac gattacaagg
     3721 aagcggaaga actgcgcgaa acatctgtcg agcctctcat cggcgcaaag aatgcggagg
     3781 acattaccat tcgcggcgga ggcacgattg atggcaacgg caagccgtgg tgggacgatg
     3841 tttattcgca ccggcatgtg ccggattttg ttgcagccaa acgtccgcgg ctgattttgc
     3901 tcgaccactg caagcatgtg ctgattgaga atgtcaccat ccagaactcc gcttcgtggc
     3961 aggtggtccc gtactactgc gatgacgtga tcatccgtaa tggaaagata cttgctccag
     4021 cgcactcacc taacacggac ggcattgatc ccttctcatc gcatcacgtc acgatttcgc
     4081 acatgacgat tgatgttggt gatgacaata tcgccattaa gtccgggcaa ccgggttcac
     4141 cggggcccga cgaccccagc acggacatta cggtcaccga ctgtaacttt ctgcatggac
     4201 atggaatgtc cattggcagc gagattgcgg gcggcgtaca gaacgtgcac gtcgcaaggg
     4261 tgcattttga cggtaccggc aatggcgtgc gcatcaagtc caaccgcgac cgtggcgcag
     4321 acatcggcaa tttcgagttt cgcgatctga cgatggaaaa cgtgggcact gcgattctga
     4381 tttcgatgta ttatccgaag attccgcaga acgacactgc gcagccggtc acgcatctga
     4441 ctccgcgttt tcacgacatt cacatcgaaa acgtgagggc gacaggcgcg aaagaagcag
     4501 gcgtgattat tgggttgccg gagatgccga ttaccacaat cacgctggac aatgtgcagc
     4561 tcagcgcaca gaagggcatg accatcagca acgctaccgt gaccgcgcac aatttccatg
     4621 tgcaggcagc cgaaggccag cctgtcattc tgctcaacaa cgcaaagctg caatgagaag
     4681 acgcgtgttc gcaaaaaact ttttgctgct tgtggtctgc gctggtgctt cttatgcgca
     4741 gactacggtc aaggcatgtg ctgtgctgaa ggccacgcac acgagcagcg acgaagcaga
     4801 agcggccgat acggcccgga tccagtcggc gctgagccaa tgctctccgg gcaaagcggt
     4861 cgtgttgcag gcatccggca gcaaggacgc atttatctct gctccgctgt tgctgcctcg
     4921 cggtgtcacg ctgtttatct cacgcggtgt gacgctgttc gcttcgcgca atctacggga
     4981 ctatgatttg catcctggca gttgtggcaa agctggatgc aaaccgttcc tttacgcgta
     5041 tcaggcggcc tggagcggcg tggccggaga aggcacgatt gatggtcagg cggacaagac
     5101 tgcatggtgg cgcattcgtg aagagtcgaa aacagctcag caccagggca acccacaggg
     5161 tgtcgtgcct gatctgattg cttcatatga gtcgcagaac ttcagcgtct acggattgca
     5221 tctgaagaac actgcgggcg acagcgtggc cgcgtacaag accatcggct ttaaggcaga
     5281 tgatttggta atggacgcac cgcatggtgg agatggaatt ctgctaagca attcgccgga
     5341 tgcctcgctc agaaacctga acattcgtgt agatggcgcg gccatagata ttcgcgccag
     5401 cattctgggc ggaacgtccc gcgttaacat cagcgggctg cacgtgaatg gcggcgaggg
     5461 aattgctttt ggagattcac aatacggcga tgttcatgct gttcacattt caaactccac
     5521 actgggcaat gccggcctga aatttgatct gcgaggagtg cgcggcggca tattgcgcga
     5581 tgtgcagttt gacaacgtat gccttgccgg aagtgtcact cctcttaccg tgcaatcgca
     5641 gagcgggacg gccagcgttt tgcccacgga ccaaagcatc cggtttgcaa atgttgttgt
     5701 acgtggacgt gggcttctaa ccgcgcatgg agttcaggaa atcctcaatg cagtttgtcc
     5761 agagacggac cagacgccca cggccatctc ctgggttgta aataccagcg caatccatca
     5821 tccgggaaca cgatcgaaat tagtcgttgc ggaggacggc agcggcgact ttcgcacggt
     5881 ccaggatgcg gtagatgcac tgcctgaaac aggtggtgac attgcggtga aacctggcgt
     5941 ataccgcgaa gttgtgacca ttcgcaagcc gcatgtgcat ctttatgggg aaaactccga
     6001 cgcgcagaag acagagattg tcttcaacaa caccggtcca cataatggcg gcaccttcaa
     6061 cagcgcaacc gtctttgtag aagccgacag cgacagcatc tcgaatctga ccatcgccaa
     6121 cgacgcaggc agcggcaagg ggcaggccgt ggcccttgcc gttacaggcg ac
//
LOCUS       DASRBV010000220         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_471773, whole genome shotgun sequence.
ACCESSION   DASRBV010000220 DASRBV010000000
VERSION     DASRBV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471773"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFA02_08240"
     CDS             complement(<1..560)
                     /locus_tag="VFA02_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09880.1"
                     /translation="MADQLYLSLWYPNFRLTSLGPALLGVMRQFHLAGGDRLVRSAAA
                     YPISWNEAPQYQRIYDEDETDAAAPEEAVPAALELLHEDFAYEFELTWDLWTPEVGGG
                     LDPVWRKEPRVVRIVGFGPEFDEGAYEQNGQIRVDFGLDTPFLHEDVDLDPDTAVRVK
                     ENVQLLIDFTNGVQQHCGISSRLLWS"
     gene            complement(560..991)
                     /locus_tag="VFA02_08245"
     CDS             complement(560..991)
                     /locus_tag="VFA02_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HZU09881.1"
                     /translation="MATASDFVGTWKLLDYSFLYDDGRVEKPWGENVVGYLLYSAEGY
                     MSGNLSPAGRRHNVEKAGDRKPSRKRDYIAYAGPYTVKGDTVTHHVEVSLFPNWLGTA
                     QLRYHKLEGDTLVLRTPPVLSDDGMVAVQLTWQRVKHSKIK"
     gene            complement(997..1800)
                     /locus_tag="VFA02_08250"
     CDS             complement(997..1800)
                     /locus_tag="VFA02_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU09882.1"
                     /translation="MSSVPEIIVEEKNGHKGPQPYIAFEHVSKSFGSNVVLDDVSFYV
                     LPGETLCILGRSGVGKSVSLQQIMGFLKPDSGKILVAGEDICCFTEEQMQAIRRKVTM
                     VFQNGALFDSLTVGENVAFPLRERGELREDQIQQIVNGLLEMVGVAEMKDLLPSDLST
                     GMKRCVAIARALAAQPEAILYDEPTTMVDPLIARLLGDVIQKLKHQLHLTSIVVTHDM
                     RFAKKLADRVLFLHQAKAVFFGTMQEMEHSQEEVVRQFLELDELVLPEA"
     gene            complement(1832..2425)
                     /locus_tag="VFA02_08255"
     CDS             complement(1832..2425)
                     /locus_tag="VFA02_08255"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZU09883.1"
                     /translation="MNGFVLAGGRSTRMGRDKALVPYHGRPLIEHAVTLLRSAGLEPH
                     IVGTRPDLAQYAPVLSDVHQGCGPLGGIEAALDVSSSEWSLFLAVDLPLMPPAFLRYL
                     RKRVSITEASATVPTFAGRPMPLCAVYHRKLLAGIRNALQASEYKVVRAIERAADGNL
                     DLFSVEAVVSARDDLSALPPHHWFQNVNTPSDLALIS"
     gene            complement(2422..3792)
                     /locus_tag="VFA02_08260"
     CDS             complement(2422..3792)
                     /locus_tag="VFA02_08260"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZU09884.1"
                     /translation="MSASVEYARANSARFLDELKSLLRIPSISTLPEHKGDVRKAAEF
                     LVTELKRIGMENVRLIETAGHPLVYADWLHAAGKPAVLCYGHYDVQPPDPLDEWISPP
                     FEPTERNGDLYARGAVDDKGQMYMHVKALESLFAAHRGKLPLNVRMILEGEEEVGGEA
                     IAKFVREHPEQLKADFALVSDTEMFAPDLPTLCVGLRGMIYTEIEARGAKTDLHSGIY
                     GGATPNPFVALAQIIAQLKDKNEHILIPGFYDKVLAPSADELKAWKRLPFDEEHYRRT
                     EVGSSQLTGEPGYSVLERTWARPTLDVHGMPGGFTGAGAKTVIPAKAVAKISMRLVPD
                     MTPQETFAQYKKYVESLKPAGIELDVRLVHSGDPIVIGTDNPYIQAATKALKQVWKKD
                     TVFVRGGGSIPIVGDFERHLKLPTVMMGFGLPDDNLHAPNEKFNLANFYRGIESIITF
                     IEELGK"
     gene            complement(3840..>4252)
                     /locus_tag="VFA02_08265"
     CDS             complement(3840..>4252)
                     /locus_tag="VFA02_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZU09885.1"
                     /translation="RWLLPVWEKLFGPVTPEQWATIHFYIRKAGHFVGYGLVSLAFFE
                     GWRATFSEKSKGKGWLFALAAPLALTSTLVLASWDEWHQSLLPGRTSSIFDVGIDLSG
                     AIAAHVLLLVVLLVVWRIRVARAKRVSTAIPASR"
BASE COUNT          958 a         1201 c         1170 g          923 t
ORIGIN      
        1 tcagaccaca gcaggcggga agagatgccg cagtgctgct gcacgccgtt ggtgaagtca
       61 atcaatagct ggacgttctc tttcacgcgg acagcggtat cggggtcaag gtctacgtct
      121 tcatgaagaa atggcgtgtc cagaccaaaa tcaacacgaa tctgtccgtt ctgctcgtat
      181 gcaccttcgt cgaactccgg cccgaagccg acgatacgca cgacgcgcgg ctccttgcgc
      241 cagacggggt cgagaccgcc gccaacttcc ggcgtccaca ggtcccatgt cagctcgaat
      301 tcgtaggcaa aatcttcgtg cagaagctcg agagcggctg gaacggcctc ttccggcgca
      361 gcggcatcgg tttcatcttc gtcgtaaatg cgctgatatt gcggggcctc gttccacgag
      421 atgggatagg cagcagccga tcgcaccaga cggtcgcctc cggcaagatg aaattgacgc
      481 atgactccga gcaatgccgg cccaagagag gtcaggcgga aattggggta ccacaggctt
      541 agatagagtt gatcagccat tactttattt tagagtgctt tacccgttgc caggtaagct
      601 gaacggcgac catcccatcg tcagaaagca caggcggagt gcgcaggacc agagtgtcgc
      661 cttcaagctt gtgatagcgc agttgcgccg tgcccagcca gttcgggaaa aggctcactt
      721 ccacatgatg cgtgactgta tcgcccttga ccgtgtacgg gccggcgtag gcgatgtaat
      781 cccgcttgcg gctgggtttt ctgtctccgg ctttctctac attgtggcgg cggcccgcgg
      841 ggctgaggtt tccgctcatg tagccttcgg ctgaatagag caaataaccc acgacatttt
      901 cgccccaggg tttttcgacc ctgccatcgt catacaaaaa ggaatagtcc agcagcttcc
      961 acgtgccgac aaagtcgctg gctgtagcca taggattcat gcctccggaa gcaccagttc
     1021 atccaattca agaaactggc gcaccacttc ttcctgggag tgctccattt cctgcattgt
     1081 gccgaagaag acggcctttg cctgatgcag aaagagcacg cggtcagcca gttttttggc
     1141 gaaacgcatg tcatgtgtca caacaatgct ggtgaggtgc aattgatgct tcagtttttg
     1201 aattacatcg ccgagcaggc gcgcaatcag agggtccacc atggttgtcg gctcgtcata
     1261 aagaatcgcc tcgggctgtg cagccagggc gcgcgcaatg gcgacgcacc gcttcattcc
     1321 cgtggaaaga tcagatggca gaagatcctt catttcagca acgccgacca tttccagaag
     1381 accattcacg atttgctgga tctggtcttc gcgcagctcg ccgcgctcgc gcagaggaaa
     1441 agcgacattt tcgcccacgg taagggaatc gaagagcgcg ccgttctgga agaccatggt
     1501 caccttgcgc cggatggcct gcatctgctc ttccgtaaaa cagcagatgt cttcgcccgc
     1561 gaccagaatt tttccggaat caggcttgag gaagcccata atctgctgga gcgagactga
     1621 tttaccgaca ccgctccgtc caagaatgca cagggtttcg ccaggaagga catagaagct
     1681 gacatcgtcg agcacaacat tgctgccaaa tgatttggag acgtgctcga atgcgatgta
     1741 aggttgcggc cctttgtgac cgtttttttc ttccacgatg atttcaggaa cgctgctcaa
     1801 tgtctgctcc tgtgtatcac ttgacctgtt ctcagcttat cagcgccaaa tcagatgggg
     1861 tatttacgtt ctgaaaccaa tgatgcggcg ggagtgctga aagatcatcc cgcgccgaaa
     1921 ccaccgcttc cacgctgaac agatccagat ttccatcagc agccctttcg atagcgcgaa
     1981 caaccttgta ttcactagct tgcagagcat ttctgattcc ggcgagcagc ttgcgatgat
     2041 agaccgcaca aagcggcata ggccgccctg cgaaagtcgg gaccgttgcc gatgcttccg
     2101 tgatgctcac gcgttttctt aagtagcgaa gaaaagcagg cggcataagc ggcaaatcta
     2161 ccgcaagaaa caggctccat tcactgctgc tgacgtcaag cgccgcctcg attccaccca
     2221 gcgggccgca tccctgatga acgtcggaaa gcacgggcgc atattgggcc aggtctggcc
     2281 gtgtgccaac aatatgcggc tccagcccgg cggaccgcag cagtgttacg gcgtgttcta
     2341 tcaggggccg cccatgatat ggcacgagcg ccttgtcgcg gcccatgcgt gtgctgcggc
     2401 ctccggccag aacaaagccg ttcattttcc cagctcttca atgaaagtaa tgatggactc
     2461 gatgccacga tagaagtttg ccagattgaa cttttcattg ggtgcgtgca gattatcgtc
     2521 aggcaggccg aagcccatca tcacggttgg aagcttcaaa tgccgttcaa aatcgcccac
     2581 gatcggaatc gagccgcccc cgcgaacaaa gaccgtgtct ttcttccaga cctgcttcag
     2641 cgctttggtt gcggcctgga tgtagggatt atctgtgcca atcacgatgg ggtcgccgga
     2701 atggaccagg cgcacatcaa gctctatgcc cgcaggtttc agcgattcga catatttttt
     2761 gtattgcgcg aaggtctcct gcggcgtcat gtccggcaca agacgcatgg atattttcgc
     2821 caccgctttt gcaggaatca cggtctttgc gccagctccg gtaaagcctc cgggcattcc
     2881 gtggacatca agtgtggggc gcgcccaggt acgttccagt acggaatacc ccggctcgcc
     2941 ggtcaactgc gatgagccta cttcggtccg gcgatagtgt tcttcatcga aaggcagacg
     3001 cttccacgcc tttaactcat ccgcacttgg ggcgagtact ttgtcataaa aaccagggat
     3061 gagaatgtgc tcgtttttgt ctttgagttg cgcgataatc tgcgccaaag ccacaaaagg
     3121 attgggcgtg gccccgccat agattcccga atgcaggtct gtttttgcgc cgcgcgcctc
     3181 aatttcggta tagatcatgc cgcgcagccc aacgcaaagc gtcggcagat ccggagcgaa
     3241 catttctgta tcagaaacca gggcaaagtc ggccttcaac tgctccggat gctcacgcac
     3301 aaattttgca attgcctcgc cgccgacctc ttcttcgcct tccagaatca tgcggacgtt
     3361 caggggcaat ttgcctcgat gcgcggcaaa gagcgactcc agcgccttga cgtgcatgta
     3421 catctggcct ttgtcgtcca ccgcgccgcg cgcataaaga tcgccattgc gctcggttgg
     3481 ttcaaatggc ggggaaatcc actcatcgag cgggtccggc ggctgcacat cgtaatgccc
     3541 gtagcagagg accgctggtt tgcccgcggc atggagccag tcggcgtaga ccagcggatg
     3601 ccccgcagtc tcaatcaggc ggacattttc cattccgatg cgctttaatt ctgtcaccag
     3661 aaactcagca gctttacgca cgtcgccttt gtgctccggc agtgtggaaa tggaaggaat
     3721 tcgcagcaat gatttcagct catccagaaa acgcgcggaa ttcgcgcgag cgtattcaac
     3781 agaagcagac atggtttctc ccaatgaagg aacagatgag tatacggaaa ctgtgtgatt
     3841 taacgcgatg ccgggattgc tgtggaaaca cgttttgcgc gcgccactcg tattcgccac
     3901 actacaagca gcaccacgag aagcaggaca tgggcggcga tggcgccgga aagatcaatc
     3961 cctacatcga agatggaact ggtgcgtccg ggcaaaaggg actggtgcca ttcgtcccat
     4021 gatgcaagca ccagcgtgga tgtcagcgcc agaggagcgg ccagcgcaaa gagccagccc
     4081 ttgcctttcg atttttcaga aaaggtggcg cgccatccct caaagaaagc gaggcttaca
     4141 aggccatatc ccacaaagtg cccggcctta cggatgtaga aatggatggt ggcccattgc
     4201 tctggtgtga ccgggccaaa aagtttttcc caaacaggga gcagccagcg gc
//
LOCUS       DASRBV010000221         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_163802, whole genome shotgun sequence.
ACCESSION   DASRBV010000221 DASRBV010000000
VERSION     DASRBV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163802"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFA02_08270"
     CDS             complement(<1..308)
                     /locus_tag="VFA02_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09886.1"
                     /translation="MNAWRLPANRVPSSYFNALQKARRACSKMIGMGTAIEKLRLSEI
                     SPRIVQSEIRSMTVECDRMGGVNLAQGVCDTEVPSPVVESAIQAIRDGANIYTRLDGI
                     A"
     gene            307..2139
                     /locus_tag="VFA02_08275"
     CDS             307..2139
                     /locus_tag="VFA02_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU09887.1"
                     /translation="MRNILRLLYYVRHYWWQALASVVLMAAVGLLEAFRVLLVKPIFD
                     HVLNPGGIQQQIQLYRVPGTHYQFTLQQFVPSYFHNEWTIVAFALVISTLLKSVCDYT
                     GTYLVNYAGFGMITDLRDDLYEAILRRSVSFFQKHATGTLLSTLINDVERVQYAMSSV
                     LSEFLQQVFTLIFTMGVVIALGGKLAWALLIFVPIVIGSIRRIGRDVRQTTRKGQDRL
                     AEIQNILHETITGNRIVKAFNMEFWELLRFRDAARRLLKANLRSVRAQAISSPLMDSI
                     GAIAIALLLLVGRGQIKSGAMTEGTFFAFIVALFKLYDPVRRFAIFYNNFQQALGASS
                     AIFKFMDAQDELVEKHRAYTLKGFQDSIRLENVNFSYRDDEGEKQVLYDINLEIRRGE
                     VVALVGPSGAGKSTLVNLVPRFFDVTSGRILIDGHDLRDVSIRSLRDQIGKVTQETIL
                     FNDTVRNNIAYGQPDVPMYRVQEAAKAALAHDFIMRMPDGYNTIIGEKGFRLSGGERQ
                     RLAIARAILKNAPILILDEATSALDTESEALVQAALSNLMQGRTVVVIAHRLSTVRRA
                     DRIAVMEHGCITAIGPHDELLRISPTYQKLYQLQFMDEPAAALQ"
     gene            2146..3051
                     /locus_tag="VFA02_08280"
     CDS             2146..3051
                     /locus_tag="VFA02_08280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZU09888.1"
                     /translation="MSSAKVFSMTGFARVSVRISDTLGYTLGLKSVNHRFLDLHLRLP
                     SGTEPLEMQLRKALKEKLRRGHVEVTLSIDRGVKAEAQYDTEIIAAYISAFRSAAARH
                     GLSGEPDLNAIFRLPGVLTGDTRNSEEEIQAVEVSVIEQLDGLIASLNTMRAAEGEAL
                     ANELRKGLKRLAEYVEQVAAFREDVQKVYFERLSQRISEMTAGSFDHERVLQEAAILV
                     ERSDVEEEIARMRTHIAHFESLLDEGGEIGKKLDFLLQEMNREANTLLSKTSGISGNG
                     AKITEIGLGMKSEIEKAREQVQNLE"
     gene            3175..3885
                     /gene="gmk"
                     /locus_tag="VFA02_08285"
     CDS             3175..3885
                     /gene="gmk"
                     /locus_tag="VFA02_08285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZU09889.1"
                     /translation="MAGILFIISAPSGSGKSTLVNQIRSLVDNLEFSISYTTRAPRGS
                     EEDGREYHFITREAFERMVRNDEFLEYADVFGNYYGTARRSLAEAQAHGKDLLLDIDV
                     KGAAQVRGKVPDAVSIFVMPPSPDVLATRLRNRSRAEGSIREDVIERRLAEAKREIEN
                     YREYGYILVNEVLSRAEEELLAIVTSERLLREGRRPGPDQERLVKLAEGCRQQNAEAR
                     VKPVLAAFGLLAPETAKM"
     gene            3915..4148
                     /gene="rpoZ"
                     /locus_tag="VFA02_08290"
     CDS             3915..4148
                     /gene="rpoZ"
                     /locus_tag="VFA02_08290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZU09890.1"
                     /translation="MSVEQTFDSNFRYVLVAARRARQLQNGSTPLVPTKSSKACRIAQ
                     EEIAAGKIGYVKGDSPIIKPEVAGADIPRFAIS"
     gene            complement(4489..>4887)
                     /locus_tag="VFA02_08295"
     CDS             complement(4489..>4887)
                     /locus_tag="VFA02_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09891.1"
                     /translation="YMCAATEKVPDAQTLQQLQMRADHASPKEQCFLYAEIVHDMTEI
                     AGQEFSNGNFAQASQTLKGIQLYAQKIQMGMADDTKKLKNAEILMRHTAYRLNEILHS
                     ASLDDRPVLESTLKQLDKVQSELMMQVFRH"
BASE COUNT         1088 a         1313 c         1394 g         1092 t
ORIGIN      
        1 gcaatgccgt caagccgtgt atagatgttg gccccatcac ggatggcctg aatcgcgctt
       61 tccaccacag gcgacgggac ttccgtatca cagacccctt gtgccagatt cactccgccc
      121 atgcggtcac actccacagt catggaacgg atttcagact gcacgatgcg tggagaaatc
      181 tcgctcaaac ggagcttttc gatagcggtg cccatgccaa tcatcttaga acaggcgcga
      241 cgcgcctttt gcagggcgtt aaaataactg gatggaactc tattggcagg caggcgccag
      301 gcattcatga ggaacatcct gcgtctgctg tattatgtgc gccattattg gtggcaggca
      361 ctggcctctg tcgttcttat ggcagcggtc gggcttctgg aagcatttcg cgtgctcctg
      421 gtcaagccca tctttgacca cgtgctgaac cccggcggca ttcaacagca gatccagtta
      481 tatagagtcc ccggaacgca ttaccagttc accctccagc agtttgttcc ttcctacttt
      541 cacaatgaat ggaccatcgt cgcttttgcg cttgtgattt ccacgctgct caaaagcgtc
      601 tgcgactaca ccggcaccta tcttgtgaat tatgccggct tcggcatgat taccgacttg
      661 cgcgatgatc tttacgaagc aatcctgcgg cggtcggttt cattctttca gaaacatgca
      721 acaggtactt tgctttccac gttgattaac gatgtggagc gcgtgcaata cgccatgtcg
      781 tccgtgctgt cggaattttt gcagcaggtc tttaccctga ttttcaccat gggcgtagtg
      841 attgcgctgg gcggcaagct ggcctgggct ctgctgatct ttgttccgat tgtgattggc
      901 tccattcgcc gcattggccg cgatgtgcgc caaactacgc gcaaggggca ggaccgtctg
      961 gccgagatcc agaacattct gcatgagaca atcaccggta accgcattgt gaaggccttc
     1021 aacatggagt tctgggagct tctgcgcttc cgggacgcgg cgcggcggct gctgaaggcg
     1081 aacctgcgct ccgtgcgtgc acaagccatc agctctccgt tgatggactc cattggcgcc
     1141 attgccattg ctctgctgct tctggtcggg cgcggacaga tcaaaagcgg cgccatgacc
     1201 gaaggcacat tctttgcgtt tatcgtggcg ctgttcaagc tgtatgatcc ggtgcggcgg
     1261 tttgcaattt tctataacaa cttccagcag gcgctcggcg cgtcatcggc catctttaaa
     1321 ttcatggatg cgcaggacga actggttgag aaacaccgcg cttatacgct gaaaggcttt
     1381 caggattcaa tccggctgga gaacgtcaac ttttcctacc gcgatgatga gggcgaaaag
     1441 caggtcctct acgacatcaa tctcgaaatc cggcggggcg aggtggttgc cctcgtaggg
     1501 ccgagcggag ctgggaaatc cacgctggtc aacctggtcc cccgcttctt tgatgtgacc
     1561 tcaggacgca ttctgattga tggccatgat ctgcgcgatg tcagcatccg ctccttgcgt
     1621 gaccagattg gcaaggtcac acaggaaaca atcctgttta atgacacggt gcggaacaac
     1681 atcgcctacg gccagccgga cgttccaatg taccgcgtgc aggaagcagc aaaggcagcg
     1741 ctggcgcacg atttcattat gcgaatgccg gacggctata acaccatcat cggcgagaag
     1801 ggcttccggc tttctggtgg ggaacggcag cggcttgcaa ttgcgcgcgc catcctgaaa
     1861 aatgcgccga ttctgattct ggacgaggca acctcggcgc tggatacgga aagcgaggcg
     1921 ctcgtgcagg cagcattgag caacctcatg caggggcgca cggtggtggt gattgcccat
     1981 cggctctcaa ccgtccgccg cgccgaccgc attgccgtga tggagcacgg gtgcattacg
     2041 gcgattggcc cgcacgatga gctgttgagg atttcaccga cataccagaa gctatatcag
     2101 ttgcagttta tggacgagcc agcggccgcc ctacagtaag agactatgag ttctgccaaa
     2161 gttttttcca tgacagggtt cgcgcgggta tctgtgcgca tttcagacac gctcggctat
     2221 accctgggcc tgaagagcgt aaaccaccgc tttctcgacc tgcacctgcg cctgccctcc
     2281 ggcacggagc ctctggaaat gcagttacgc aaggcgctta aggaaaagct caggcgtggg
     2341 catgtggaag tgacgctttc aatagaccgc ggcgttaagg ccgaggccca gtatgataca
     2401 gagatcatcg cagcatatat ctctgcgttt cgctctgcgg cagcacggca tggtctttca
     2461 ggagaaccgg acctgaacgc catcttccgg ctgccaggcg tgctgaccgg agatacacgc
     2521 aattctgaag aagagataca ggccgtggaa gtctccgtga tagagcaatt ggatggactg
     2581 attgcatctt tgaacacaat gcgcgcagcg gagggtgaag ctcttgccaa tgaactacgc
     2641 aaagggctga agcgtctggc cgaatatgta gagcaggtgg cggcctttcg ggaagatgta
     2701 cagaaggtgt attttgaacg gctcagccag cggatatctg agatgacggc aggcagcttt
     2761 gaccatgagc gggttctgca ggaagcagcg attctggtag agcgcagtga tgtcgaggaa
     2821 gagattgcgc gcatgaggac gcacattgca catttcgaat cactgctgga tgagggcggc
     2881 gaaatcggca aaaaacttga ctttctgttg caggagatga accgcgaagc caacaccctt
     2941 ttgtcgaaga ccagcggaat ctccggcaat ggagcaaaaa tcacagaaat cgggctgggg
     3001 atgaaatcag agatcgaaaa agcgcgtgag caggtgcaga acctcgagta gctgccggtt
     3061 tttagttgcc ggtttccagt tgtcagttgt tcgtccatag tgatggacaa gaactgggga
     3121 ccagcgactg gaaactgtgc actagggact aaaaactaaa ttaaggacca tcaattggca
     3181 ggcattttat tcatcatttc cgcgccctct ggatcgggta aatccacgct tgtgaaccag
     3241 attcgctcgc tggtggacaa ccttgagttc tccatctcct acacgacccg tgcgccgcgc
     3301 ggctcagaag aagatggccg cgagtaccac ttcatcacgc gcgaagcgtt cgagcgcatg
     3361 gtccggaatg atgagtttct ggagtatgcc gatgtctttg gaaattacta tggaacggcg
     3421 cggcgttctc tggctgaagc gcaggcgcat ggaaaggacc ttctgctgga cattgacgta
     3481 aaaggagcag cgcaggtgcg cggcaaagtg cccgatgcgg tcagcatttt cgtcatgccg
     3541 cccagcccgg acgtcctcgc cacccggctg cgcaatcgaa gccgcgccga aggcagcatc
     3601 cgcgaggacg tgattgagcg ccgtttggcc gaagcaaaac gggagattga gaattaccgc
     3661 gaatacggct atattctggt caacgaagtt ctgtcccggg cagaagagga gcttctggcc
     3721 attgttacca gcgaacgcct cctgcgggag ggccgcaggc ccggccctga ccaggaacgg
     3781 ctggtaaaac tagccgaagg ctgccggcag cagaatgcgg aggcacgcgt aaaacccgtg
     3841 cttgccgcct tcggtctgtt ggccccggag acagcaaaga tgtagcccca tcggtcatat
     3901 tggaggacca ggcaatgagc gtggaacaga ccttcgacag caacttccgc tatgttctcg
     3961 tggccgcccg ccgcgcccgt caactgcaaa atggatcaac accgctggtt ccaaccaagt
     4021 ccagcaaagc atgccgcatt gcgcaggagg aaatcgccgc aggcaagatt ggatacgtca
     4081 aaggcgattc tcccatcata aaaccagaag tcgcaggggc cgacattccg cgcttcgcga
     4141 tttcctagcc tgggaccaca ctgccgaatg gccctgttta cgagagtacg cgcagggccg
     4201 cgtcaaaagt gacgacagtg tggccatgcg tctgcatggg cataggccag aagacgacag
     4261 tcgcctcccc aactttgagg cctgttactt tgctaaaaac aaggtcaaac aggttttcgt
     4321 ccgttggaaa ggcaggccct ggtttttctt ctgctatgga taaatgggcc ggttacgaac
     4381 tatccctttt caggtctggc atatgtcttt tcgatgtata gggttccgga atgtccacgg
     4441 tcattctgaa aggcgtgaca tgatcatggc agaagcactg gaacttcgtc agtgcctgaa
     4501 tacctgcatc atcagttccg actgcacctt gtccagttgc ttcaacgtgg attccaggac
     4561 gggccggtcg tccagcgacg cgctgtgcag gatctcattc agccgatagg cggtgtggcg
     4621 catcagaatc tctgcatttt tcagcttttt cgtatcgtcg gccatgccca tctgtatctt
     4681 ctgggcgtag agctggatgc ctttcagtgt ttgtgatgcc tgcgcaaagt ttccgttgga
     4741 aaactcctgc ccggcaatct ccgtcatatc atgaacaatt tcagcataga gaaaacattg
     4801 ttccttagga gaggcgtgat cggccctcat ctggagctgc tggagtgtct gggcatcggg
     4861 gaccttttcc gtggcggcac acatata
//
LOCUS       DASRBV010000222         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_317351, whole genome shotgun sequence.
ACCESSION   DASRBV010000222 DASRBV010000000
VERSION     DASRBV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317351"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..2691)
                     /locus_tag="VFA02_08300"
     CDS             complement(364..2691)
                     /locus_tag="VFA02_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZU09892.1"
                     /translation="MLRSRLLMCWLAMSLVSGMGFVLSAETVPSGTVLEIRLKQPVRS
                     YSTHEGTRITGILISPVNEEGKILLPIGTTIEGTVTDVHKVGIGIMHETALIEMRFDH
                     LVLADGSTFPVQCRITEVENARESVDKEGRIHGIRSTATLSHRTSGVMNSFAFGDPIA
                     AIFATAGSAAVLRFSEPEISFPEGTEMMAELTAPLNLPGTSAQVVPPIAATDAERDGL
                     REIVRALPFRTYTDGSNIPSDMTNLIFIGSSDALERAFAASGWVRSDPLTSETAYRTI
                     RSIAENQGYRAAPMSTLLLDGKRPDYTYAKTLDTFSARHHLRIWKTSEKWNGETIFTS
                     SSTHDIGIGFSKANKTFIHLIDTHIDNERAKVVNDMIYTGCVAGIQLVTRPWIPKDAK
                     NGTGEPLITDGRIAVLRLNDCRAPVETEALQAHTLPEHGNRFDRVARQTVLTLKNNVL
                     RDNVGVMAYSGIHTLTSKKKSGPRPVRQMEVGGSEYTIEETGSARTAYDFSPPVASTE
                     RKKGPRVPDWTPPTIELGIRGGWRGYAGGNGGSIAYLFQSVSDPDQSYFLVAGNTLDN
                     GWSIGGSITINANKYFSHEFSYDHSFTTFTLGYVTVDNDSTSESIKDDFEFDPSGLQT
                     SQFTYNLLWNLRPKTSRWRPYFAVGPTLQLMHLDEAPIKKAPKWFKLGLSNIGLLTAA
                     YDFGSTPALEGGGIFQAGLNYGGGIRYRLTPRWMARVDWRETLTSQPDFWSKSKNDLF
                     GDTSQDEYQLQLVLGPELEGPMRQEHVTGGISFTF"
     gene            complement(2728..3183)
                     /locus_tag="VFA02_08305"
     CDS             complement(2728..3183)
                     /locus_tag="VFA02_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09893.1"
                     /translation="MSRKYFAAAFLISWFLLGAVHLNAGEKKWQTGTLLSVEHREQPA
                     EMTKRERTETSTDSNGNTSSTTTTTLAPGYVHQWIYYTISTEKSDYVVRQEINFPWTK
                     TVKAETGDTIQCAVDGKKLYIKNPDGNTIKTDIMKTTAHIAAPSQSPQN"
     gene            complement(3343..5451)
                     /locus_tag="VFA02_08310"
     CDS             complement(3343..5451)
                     /locus_tag="VFA02_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZU09894.1"
                     /translation="MPVDEMTERLHWFQLDRREFLKLCGGGLLVRLTGLRSDAQESGR
                     SFSEHALPQEISAWLHIDEEGKTTVFTGKVEIGQNIRTSLAQLVAEELRVPFGFITMV
                     MGDTDLVPWDMGTFGSRSTPTMGPQLRNMAAAARQVLVETAARRWNADPAALKAENGK
                     VTDPKTGQSLSYGEITRGEKLMQTVAADPALTPAIEWKIAGKAIPKVDGRDFVTGKHQ
                     YPSDIFRPGMQFGKVLRPAGFHATLVSVDTNAAEKMPGVKVVRDGDFVGVVAPDAWMA
                     EQALAAIQAKWNVPAQTSNQELFDYLRNHPQDGGPSEPHVTGSVEQAMTSAEVKLQQH
                     YTVQYIAHAPLEPRAAVAEWSGDRPTVWTGTQRPFAVRDELAAAFRIPARNVRVIMPD
                     MGSAYGGKHSGECAVEAARLAKAVGKPVKLVWTREEEFTWAYFRPAGWINIRSGANKD
                     GKLIAWEHHNYNSGPSAINTPYDVANQIIQFHPAQSPLRQGSYRGLAATANHFARESH
                     MDELAYAIGIDTLEFRLKNLSDPRLRTVFQAAADRFGWGKQKPVAGRGFGIAGGVEKG
                     GHVATCVEVEVDSANKHVHLLRVVQAWESGAIVNPNGLRNQIEGALVQAIGGALFEAV
                     QFKDGRILNPHFAQYRVPRFRDMPQIDVVLIDRKDLPSAGAGEIGIVGLAPAVSNAIF
                     AATGKRLRDMPMAAGKDLMG"
     gene            complement(5441..>5865)
                     /locus_tag="VFA02_08315"
     CDS             complement(5441..>5865)
                     /locus_tag="VFA02_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZU09895.1"
                     /translation="LLSVLRNQLHLTGTKYGCGEGQCGACTVLINGAARRSCQMRVAS
                     ATDADITTIEGLERNGTLHPVQQAFLVEEAFQCGYCTSGMILSATTLLRDHPQPTDQQ
                     IIEYMNGNLCRCGTYPRIVAAIRRAAEIQAKGGTSHAG"
BASE COUNT         1243 a         1646 c         1590 g         1386 t
ORIGIN      
        1 tcctccagga gcacaatgga cggattcgct tcccgcaccg gaattgttcg cgggtccggt
       61 tgttcgtgga gcgccgcagt ggaacacgaa acttctgcag gcaaattgag tagactcgtt
      121 gcggcgaagc atggtctcgg cttatgcgcg ccatttcgca tgtggaaggc cgtagcgcga
      181 aatcaagatt tgacaactcc tgccctttct cgtggagggt ttctattatg ccatccttca
      241 ccccacaacc ctacaaccct gcaaccagat actttcttta gggcagagct tctgttacca
      301 gcatcatgcc gatgaccaca tacacagtgc tgcatgctaa agcttatcgg gctatcgagc
      361 ggcctaaaac gtaaaagaga tacctccggt aacgtgctcc tgacgcatcg ggccttccaa
      421 ttcagggccc aagaccagtt gcaactgata ttcgtcctgg ctggtatcgc cgaaaagatc
      481 gtttttggat ttggaccaga aatcgggttg cgacgtcagg gtctcgcgcc agtctacccg
      541 cgccatccat cgcggagtaa gacgataccg gatgccgcca ccatagttca gaccagcctg
      601 aaagatacct ccgccttcga gagcaggcgt gctgccaaaa tcgtaggccg cagtaagcag
      661 cccgatattg ctcaacccca gcttgaacca tttcggcgcc tttttgatag gggcttcatc
      721 caggtgcatg agctgcaatg ttggaccaac cgcgaagtaa ggccgccagc gtgatgtttt
      781 gggccgcagg ttccagagca aattgtaagt gaattgcgag gtctgcagac cggacgggtc
      841 aaattcaaaa tcatctttga tggattcgct ggtggagtca ttgtccactg tgacatagcc
      901 cagcgtgaat gtagtgaagc tgtggtcgta gctgaattcg tgcgagaagt atttgtttgc
      961 attgatggtg atggacccac cgatgctcca gccattgtcc agagtattgc cggcaactag
     1021 gaaataggat tggtccggat cacttacaga ctggaacaga tatgcaatcg agcccccatt
     1081 ccctcccgca tagccgcgcc atccgccacg aataccaagt tcaatggtag ggggtgtcca
     1141 gtccggaacg cgcgggcctt tctttctctc cgtagaagcc acaggaggag aaaaatcata
     1201 ggcggtcctc gctgatccag tctcttcaat tgtgtattcg cttccgccaa cttccatctg
     1261 gcgcacaggc cgtgggcctg atttcttttt ggaagtcaaa gtgtgaatgc ccgaataggc
     1321 catgacacca acgttgtccc gcaggacatt atttttgagg gtgagcaccg tctggcgtgc
     1381 gacccggtca aagcggttgc catgctcggg caaagtatgg gcctgaagcg cttctgtctc
     1441 aacaggcgcg cggcaatcat tcaggcggag tacggcgatt ctcccatcag taatcagcgg
     1501 ctcgcctgtg ccgttttttg cgtctttggg gatccatggg cgggtaacga gctggatgcc
     1561 ggcaacacat ccggtataaa tcatgtcatt cacaactttg gcgcgctcat tatcaatgtg
     1621 tgtgtcaatc aggtggatga aagtcttgtt tgctttggaa aagccgatgc ctatgtcgtg
     1681 tgttgaagat gacgtgaaga tggtctcgcc attccatttc tcacttgtct tccagatgcg
     1741 cagatggtgc cgggctgaaa acgtatcgag cgtcttggca taggtgtaat cgggccgttt
     1801 gccatccagc agcagggtag acataggcgc cgcgcgatat ccctggtttt ccgcaatcga
     1861 acgaatcgtg cggtatgcag tctctgacgt aagcgggtcg gagcgcaccc atccgctggc
     1921 tgcaaaggcg cgctccagcg catcgctgct gccaatgaag atgagattgg tcatgtccga
     1981 tggaatgttg gagccatcgg tgtaggtgcg gaacggcaat gctctgacaa tttcccgcag
     2041 gccgtccctt tctgcatccg tcgctgcaat tggaggaacc acctgtgcgc tcgtcccggg
     2101 caggttgagc ggtgcagtca gctcagccat catttctgtg ccttccggga aggaaatttc
     2161 cggctcagaa aatcgcagta ctgccgccga cccggcagtt gcgaatatgg cagcaatcgg
     2221 gtcgccaaag gcaaagctgt tcatgacgcc ggaagtgcga tggctcagcg tggctgtcga
     2281 gcggatgcca tgaatgcgcc cttccttgtc cacgctctcg cgtgcgtttt ctacttctgt
     2341 aatgcggcat tggaccggga aagtgcttcc atcggcgagc accaggtggt cgaagcgcat
     2401 ctcgatgagc gccgtctcat gcatgatgcc aatgccaact ttatggacgt cagtgactgt
     2461 tccttcgatg gtcgtgccaa tgggaagcag tatcttgccc tcttcattga caggggagat
     2521 aagaataccc gtgattcttg ttccctcatg cgtggaataa gatctgacag gctgcttcag
     2581 gcggatttca aggacagtgc cggagggaac ggtttcagca gacaagacaa aacccatgcc
     2641 ggaaacaagg gacatagcca gccaacacat cagcaaacgc gaccgaagca taggacctct
     2701 cggcacaggt ggaaccacaa cgaaaagtca gttctgaggg ctttgtgaag gtgcggcgat
     2761 atgggccgtt gtcttcatga tgtctgtttt tatcgtgttc ccatcggggt ttttaatata
     2821 gagtttcttg ccatccaccg cgcattggat cgtatcgcct gtttccgcct ttactgtctt
     2881 tgtccatggg aagttaatct cctggcggac aacatagtcg ctcttctcag tagaaatagt
     2941 gtagtaaatc cactgatgga cgtaacccgg ggccaatgtg gttgtcgtcg tagacgaggt
     3001 attgccattg ctgtctgtag atgtttccgt tctctcccgc tttgtcattt ctgccggctg
     3061 ttcacggtgt tctacggaaa gcagtgtccc tgtctgccac ttcttttctc ctgcatttaa
     3121 atggactgcg ccgagcagga accatgaaat gagaaaggcg gcagcgaaat attttctgct
     3181 catacaaccc tcctgattgc gtaattccat ctgcagatat ggggccagga gtaaaacggg
     3241 atatttgaga cgttcctata ttatgcggta atcataatgc gcttctgggg tttttctcaa
     3301 gagaaccaat tcaggaatgg acggcttaca gcacatacat cgtcagccca tcagatcttt
     3361 gcctgctgcc attggcatgt cacgcaatcg ttttccagtc gcggcaaaga tggcattgct
     3421 gacagcagga gcaaggccga cgatgccaat ttcccctgcg cctgcagatg gcagatcctt
     3481 ccggtcaatc agcaccacgt caatctgcgg catatcacgg aagcgtggta cgcgatactg
     3541 cgcgaaatgc ggattcagaa tgcggccatc cttaaactgg accgcctcaa agagcgcgcc
     3601 gccgatggcc tgcaccagcg ccccttctat ctggtttcgt aagccattgg gattgacaat
     3661 cgcgcctgat tcccatgcct gcactacacg cagaagatgc acatgcttgt tcgcggaatc
     3721 cacttcgact tccacgcagg tagcaacgtg gccgcccttc tccactccgc cagcgatacc
     3781 gaagccgcgg cctgcaacag gcttttgttt gccccatccg aaccggtctg cggccgcctg
     3841 gaagaccgtg cgcaaccgcg gatcgctcag gttcttcagt cgaaactcca gagtatctat
     3901 accgatggcg taggccagtt catccatatg cgactcacgc gcaaaatggt tcgctgtggc
     3961 tgccagccca cgatacgatc cctgccgcaa aggcgactgc gccggatgaa attgaatgat
     4021 ctgattggct acatcgtagg gagtgttgat ggcggacggg cctgagttgt aattatggtg
     4081 ttcccaggca atgagcttgc cgtctttgtt cgcaccacta cggatattaa tccatcccgc
     4141 ggggcggaag tacgcccagg tgaactcttc ctctcgtgtc cagacaagtt ttaccggctt
     4201 gccgacggcc tttgccagac gcgccgcttc cactgcgcat tctccgctgt gtttgccgcc
     4261 ataagcgctg cccatgtccg gcatgatgac gcggacgttc cttgcaggaa tgcggaatgc
     4321 ggcggccagt tcatcgcgga cggcaaatgg gcgctgtgtg cctgtccaga cagtgggtct
     4381 gtctccactc cattcggcga cggcagcgcg cggttccagc ggagcatgag caatatactg
     4441 caccgtgtaa tgctgctgga gctttacttc cgcagaggtc atcgcttgct caacagagcc
     4501 cgtcacgtgc ggctctgagg gccctccgtc ttgcggatgg ttgcggagat aatcaaacag
     4561 ttcctggttt gaggtttgcg caggtacatt ccactttgcc tgaatggctg cgagcgcctg
     4621 ctctgccatc catgcatcag gcgcaactac tccaacaaaa tctccatcgc gcacaacctt
     4681 caccccgggc atcttttccg ccgcattcgt atccactgaa acaagcgttg catggaaccc
     4741 tgcaggtcgc agcactttgc cgaactgcat tccaggccga aagatgtctg acggatactg
     4801 gtgctttcct gtaacaaaat cacgaccatc cactttcgga atcgctttgc cggcaatctt
     4861 ccattcgatt gccggagtca acgccggatc cgctgccact gtctgcatca acttttcgcc
     4921 gcgcgtaatt tcgccatacg aaagcgattg tcctgtcttc gggtcggtga ccttgccgtt
     4981 ctccgccttc aaagccgctg gatcagcatt ccaccgtcgc gctgcggttt ccaccaacac
     5041 ctgccgcgcc gctgccgcca tatttcgcag ttgaggcccc atcgtcggcg tggagcggct
     5101 gccgaaggta cccatgtccc acggaacaag atcggtatca cccatcacca tggtgatgaa
     5161 accaaacgga acacgcagtt cttcggccac aagctgcgcc agtgaagtac gaatgttctg
     5221 cccgatttct actttgcccg tgaagaccgt ggttttgccc tcttcgtcaa tgtgcagcca
     5281 tgcgctgatc tcctgcggta atgcgtgttc actgaaactg cgtccacttt cctgggcatc
     5341 gctgcgcagt ccggtgaggc gaaccagcag accgcctccg cacagcttca gaaactcgcg
     5401 ccggtcaagc tggaaccagt gcagccgttc tgtcatctcg tcaaccggca tggcttgtgc
     5461 ctcctttcgc ctggatttca gccgcgcgtc gaatcgcggc cacgatgcgc ggatatgtcc
     5521 cgcagcggca gagattgccg ttcatgtact cgatgatttg ctggtcagta ggttgaggat
     5581 ggtcgcgtag taacgtggtc gctgagagaa tcatgccgga cgtacagtat ccgcactgga
     5641 aggcctcttc taccagaaac gcctgctgca ccggatgaag agtgccgttg cgctccaggc
     5701 cttcaatcgt ggtgatgtcc gcgtccgtgg cagaggcgac gcgcatctgg caggagcggc
     5761 gggccgctcc gttgatcagc acagtacagg ccccgcactg cccttctccg caaccgtatt
     5821 ttgtgcctgt gagatgaagc tggttgcgca gaacagaaag caggg
//
LOCUS       DASRBV010000223         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_266703, whole genome shotgun sequence.
ACCESSION   DASRBV010000223 DASRBV010000000
VERSION     DASRBV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266703"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFA02_08320"
     CDS             <1..387
                     /locus_tag="VFA02_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HZU09896.1"
                     /translation="AGNMRSRWTDFLTTDGEKPDRNRDTEFEAPPATRAELMALWDDG
                     WKRVFTALEPLTDADLGRTVTIRGEAHSVMQAINRQVAHYSYHVGQIIFLAKHLKSTE
                     WKCLSVPRRQSAEYNRKVQSGEASQR"
     gene            complement(490..1410)
                     /locus_tag="VFA02_08325"
     CDS             complement(490..1410)
                     /locus_tag="VFA02_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09897.1"
                     /translation="MKLRFLLPAFLVCALPLFAQSGDYAPASTVVTAIPKNGEAAALQ
                     RQNIKVTADRKQSQVTAWHPYQGRNAGLEFVVLVDDSARQSIGLQFSDLAKFLQSLPP
                     TALTGVAYMQNGRAVFAQDLTTDHALAAKALRQPQGVPGGSASPYFCLSDLARHWPVQ
                     DPKHRREVLMITDGFDPYYPRYDPQDPYLQAAISDAQRAGLIVYSIYYRNAGRFSSTT
                     YATDAGQNLLTQLTEATGGRLYWQGLSNPVSFQPFLDDLGKRLANQYELGFTVPARVK
                     AELVDLKVKTDVNNVNISAPQRVLVGGATR"
     gene            1503..2327
                     /locus_tag="VFA02_08330"
     CDS             1503..2327
                     /locus_tag="VFA02_08330"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZU09898.1"
                     /translation="MKRVLTALILVPLVLLLVFFNNLPLITAAAGVVALLAAWEYLGL
                     ADASGAKTPRIAVLICIAALFACNFERPEYMAPAIGCLAFVLFVLCAFRSPLDRVLPD
                     TAYSVFALLYVGLSLTTIPLLSARENGPSLLVFLFFVVWTGDVVALYTGRAFGKRKLA
                     PSISPNKTWEGSVGSVAGSVLITVLLYFVAGVFTNHGFDWLSYPGSIFRWTGLAVVLN
                     VAAQTGDLIESAMKRGAGVKDSGTLLPGHGGVLDRIDALLLAAPVLWYAVLLQQAL"
     gene            2433..2852
                     /locus_tag="VFA02_08335"
     CDS             2433..2852
                     /locus_tag="VFA02_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09899.1"
                     /translation="MEIFSNLVWVAISLALGTVWLIGYRHSDRENSMPGLRVQCVALA
                     MLILILLPVVSLSDDMQAMVATSEVEHVNRRIDMLPVNDHAAEPPLLFSGPVDLHTSL
                     HLRTFAWMEPVYAEARPLDGCLREMANRPPPVCALFS"
     gene            2919..4157
                     /locus_tag="VFA02_08340"
     CDS             2919..4157
                     /locus_tag="VFA02_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU09900.1"
                     /translation="MKRLLCSSAVLLFSAMLYAQTPPAPLTLQQAIDIARTKNPTLLS
                     GQQHVIATKASEITAGLRQNPNFTLSGADVSLPANNPSSPYTYVGNINRLFERGQKRR
                     WRLDVARATSDVTQSQYKDSERQTVLAVKQAFTQMLAAKAALKVAQDNLDGYRRTVDL
                     SRERLKAGDISATDFDRIDLQLAEFESDYDNAKLDLTQASDQLQLLLGADKPTETFDI
                     TGTLDPPVITQPLSQIEQEALQARPDYLAAEQSVRLADANVKLADAMGTTDPTIGGEY
                     ERVGTYNSAGFQISIPLRIFDRNQGEKQRTRYEAQSSRFAEIAARNQVMNDVDQAWAA
                     YEAAMHLAQRYNSHYVAESERVRDNLEFSYRHGGATLLDYLDALRDYRQVHLDALNAN
                     MQVWLAVHQLSFAAATEIVP"
     gene            4154..>4584
                     /locus_tag="VFA02_08345"
     CDS             4154..>4584
                     /locus_tag="VFA02_08345"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU09901.1"
                     /translation="MNQRHFVSFLLLCALPFAGCKHANEAQGPAPAPGKDVVNTVTVH
                     MQQVTDRLDLPARLMANPTGVVHVYPPVSGRILSLKVLPGQEVSKGQVIGEIQSSDAA
                     QARSDFEKAKIEAARADLQLQRAKELLQHEVLAQKDYDDLKA"
BASE COUNT          904 a         1191 c         1433 g         1056 t
ORIGIN      
        1 gcgggcaata tgcgttcgcg gtggacggat tttctgacta cggatggaga gaagcctgac
       61 cgcaatcgcg acacagagtt tgaagcccct ccggcaacac gggcggaatt gatggcgctg
      121 tgggacgatg gctggaagcg cgtttttacc gcgctggaac cgctgacgga tgccgacctg
      181 gggcgtacgg tcacgattcg cggagaggcg cattctgtga tgcaggccat caaccgtcag
      241 gtagcgcact attcgtatca tgtgggccag attatttttc tggcgaagca cctgaagtcc
      301 acggagtgga aatgcctgag tgtgccgcga aggcaatcgg ctgaatataa ccggaaagtg
      361 cagtcgggag aggcgagcca gcggtgagtt gctacccaca tctctttcca ccccaacacg
      421 caaaaatcgc gtgccgggaa cccgggtgcg agatatagag cacccggcga acgaaatgct
      481 gcacccggtt taccgggtgg caccgccgac aaggacacgt tgtggcgcgc tgatgttcac
      541 gttgtttacg tcggttttta ccttcagatc aaccagctct gctttaacgc gtgcggggac
      601 ggtgaatcca agctcgtact ggttggcaag gcgtttgcct aagtcatcaa gaaatggctg
      661 gaaggacacc ggattggaga gtccctgcca gtagagtctg cctccggtgg cctcggtgag
      721 ctgggtgagg aggttctgcc cggcatcggt ggcgtaggtg gtactgctga agcgtcctgc
      781 attgcggtaa tagatggaat agacgatcag gccggccctt tgtgcgtcgg agatggccgc
      841 ttgcagatag ggatcctgcg ggtcgtagcg agggtagtag gggtcgaatc cgtcggtgat
      901 catgaggacc tcgcggcggt gtttcgggtc ttgtacaggc cagtgcctgg ccaggtcaga
      961 gaggcagaaa tatgggctgg cgctgcctcc gggaactccc tgcggctggc gcagcgcttt
     1021 tgcggcgagg gcatgatcgg tggttaggtc ctgggcaaag acggcgcgcc cgttctgcat
     1081 ataggccaca ccggtaagcg cggtgggggg cagggattgc aggaactttg cgaggtcgct
     1141 gaattgcagg ccgatgctct gccgcgcgga atcgtctacg aggaccacaa attcaagtcc
     1201 ggcattccgg ccctgatatg ggtgccatgc agtcacctga ctttgttttc tatcggctgt
     1261 gaccttgatg ttctggcgct gcagggcggc agcttcgcca tttttgggta tggcggtgac
     1321 gacggtgctg gcgggagcgt agtctccgct ctgggcgaac agcggaaggg cgcagacgag
     1381 aaaggcagga agcagaaatc gtaatttcat tcggacactt tttcctctct attatttaga
     1441 cgctttttgc gaacaatccg gcgcagggcg cgcgcgaacg tgtattctgc catttacgaa
     1501 cgatgaaacg tgttctgaca gcccttattt tagtgccgct cgttttgctg ctggttttct
     1561 tcaacaatct tccgttgatt actgccgctg cgggcgtggt ggcgcttctg gcggcctggg
     1621 agtatctggg gctggcggac gcttccggcg caaagacgcc gcggattgca gtgcttatct
     1681 gcattgctgc tttgtttgcg tgcaactttg aacgtccgga atacatggcg cctgcgattg
     1741 ggtgtcttgc ctttgtgttg tttgtgctgt gcgcgtttcg ctcgccgctc gaccgtgttt
     1801 tgccggacac ggcgtattcg gtttttgctc tgctctatgt aggattgtcg ttgacaacca
     1861 ttcccttatt gagcgcgaga gagaacgggc catcgctgct tgtctttttg ttctttgttg
     1921 tatggacggg cgatgtggtt gcgctgtata ccggacgtgc ttttggaaaa cgcaagctcg
     1981 cgccctcgat cagtcctaac aagacgtggg agggaagcgt ggggtcggtt gcgggcagcg
     2041 tgctgattac ggtattgctg tattttgttg ccggagtatt tacgaaccac gggtttgact
     2101 ggctctctta tccggggtcc atttttcgct ggacagggct ggccgtggtg ctgaatgtgg
     2161 cagcgcagac aggagacctg atcgagtcgg cgatgaagcg cggtgccggt gttaaggatt
     2221 ccggtacgtt gctgccaggg catggagggg tgctggaccg cattgatgcg ctgctgctgg
     2281 ctgctccggt gctgtggtat gcagtgcttt tgcagcaggc gctttaggga gatccacttg
     2341 ctgcaaatct cccggacaag aaatcatttt tctgttcttt acgtcttcct tatctttggg
     2401 cgcagaggct tcgggtaaaa tcggcttatc gcgtggaaat tttctccaac ctggtgtggg
     2461 tcgccatttc attggcgctg gggactgtgt ggctgattgg ttatcgccat tcagaccggg
     2521 aaaattccat gcccgggctg cgtgttcagt gtgttgcgct ggcgatgctc attctcattc
     2581 tgctgccggt ggtttctctt tctgacgata tgcaggcgat ggtggcgaca tcagaagtgg
     2641 agcacgtgaa caggcgcata gacatgctgc cggtgaatga ccatgcagcc gaaccgccgc
     2701 tgctcttttc cgggccggtg gaccttcata cttcactgca tctccgcaca tttgcgtgga
     2761 tggaaccggt ctatgccgag gcacgtcctt tggatggatg cctgcgcgaa atggcgaacc
     2821 gtccgccacc tgtctgcgct ttattttcct gattgattca catcatttga ttcgcattct
     2881 attcaggcta ttttttgcat caggtcaggc attcgcttat gaaacgactt ctatgcagca
     2941 gcgctgtact tctgttttca gcgatgctct atgcacagac tccaccggcg cctttgacac
     3001 tgcagcaggc gattgatatt gcgcgcacaa aaaacccgac gctgctttcc gggcagcagc
     3061 atgtgatcgc cacaaaggcc agtgaaatta ccgccgggct gcggcagaac ccgaacttca
     3121 cgctttccgg cgcggacgtg agtctgccgg caaacaatcc cagcagccct tatacatatg
     3181 taggcaacat caaccgtctc tttgaacgtg gacagaaacg ccgctggcgt ctggacgtgg
     3241 cccgcgccac gtccgatgtg acgcagagcc agtacaagga ctcggagcgg cagacggtcc
     3301 tggccgtcaa gcaggccttt acgcagatgc ttgccgccaa ggcggcgctg aaagtcgcgc
     3361 aggacaatct ggacggttat cgcaggacgg tggatttaag ccgcgagcgg ttgaaggccg
     3421 gagacatcag cgcaacggac tttgaccgca ttgatctgca actggcggag ttcgagtcag
     3481 attacgacaa cgcaaagctg gatctgacgc aggcaagcga ccagctccag cttctgctgg
     3541 gagcagacaa gccgacggaa acattcgaca ttaccgggac gctcgatcca ccggtgatca
     3601 ctcagccgct ttcgcaaatt gagcaagagg cgttacaggc gcggcccgat tatcttgccg
     3661 cggagcaatc tgtgcggctg gccgatgcga atgtgaaact cgccgatgcg atgggtacca
     3721 ccgacccgac gattggcggc gagtatgaac gggtgggcac ttacaacagc gctggttttc
     3781 agatcagcat tccgctgcgc atcttcgacc gcaaccaggg cgaaaagcag cgcacgcgct
     3841 atgaggcgca atccagccgc tttgcggaaa tcgccgcgcg caatcaggtg atgaacgacg
     3901 tagaccaggc gtgggcggca tacgaggcag cgatgcacct tgcgcagcgc tacaacagcc
     3961 attatgtggc tgagtcagag cgcgtgcgtg acaacctgga attcagctac cgccatggag
     4021 gcgccaccct gctcgattat ctcgacgcat tgcgcgatta ccgccaggtg catctggatg
     4081 cgctgaatgc gaacatgcag gtctggctgg ccgtccatca actcagcttt gccgccgcta
     4141 cggagattgt tccatgaacc aaagacactt tgtttctttt ctgctgctct gcgctttgcc
     4201 atttgccggg tgcaaacatg caaatgaggc acaggggcct gctcctgctc cgggcaagga
     4261 cgtagtgaat acggtcacgg tccacatgca gcaggtgacc gacaggctgg acctgcctgc
     4321 gcgtctgatg gcgaacccga caggtgttgt gcatgtgtat cctccggtga gcgggcgcat
     4381 tctcagtttg aaagtgttgc ccgggcagga ggtcagcaag gggcaggtca ttggagaaat
     4441 tcagagcagt gatgcggcgc aggcgcgttc tgattttgag aaggcgaaga ttgaagctgc
     4501 gcgcgcagac ctgcaattgc agcgcgcaaa ggaactccta cagcacgaag tgctggccca
     4561 gaaagactac gatgatctga aggc
//
LOCUS       DASRBV010000224         6547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_621761, whole genome shotgun sequence.
ACCESSION   DASRBV010000224 DASRBV010000000
VERSION     DASRBV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621761"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFA02_08350"
     CDS             complement(<1..125)
                     /locus_tag="VFA02_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZU09902.1"
                     /translation="MAHEVPPLPYDYAALEPHIDEATMKLHHDKHHQAYVTNLNAA"
     gene            408..1415
                     /locus_tag="VFA02_08355"
     CDS             408..1415
                     /locus_tag="VFA02_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09903.1"
                     /translation="MKRGAFHSISLAVLSVFAMDQTVFANSAPPQAAAAGYTMNTFSS
                     TFHGDVDIHNTRAPGFNWYLAKFSGARTTEASSLHFNSDGSLTLTSGGDTANDAINSA
                     ADTGKPAPNDWVGTAFGGGGYFEAEFKFDPSFVMKPGAVGFPAWWLEPIEHIKRSGGA
                     HWNGQASGFEHFTEVDIFEYNQWKQHSPNIYAGTVHEWYGIHNVTCPGKAFCHASNEN
                     NATTQFANNLIVTPPGTDFRKYHKFGLLWVPATNTSQGYLQYYFDGKPTNDRITWSQY
                     DEQKHKPFMLKTSAQSIPWVFGVLDRQHLVLILGTGNGQPMTIKDVNVWQKNSDQNLK
                     Q"
     gene            1580..2689
                     /locus_tag="VFA02_08360"
     CDS             1580..2689
                     /locus_tag="VFA02_08360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU09904.1"
                     /translation="MERIRTSLRKKRDKPRRYVIAVASLGDYRARVVREIRSRLGSSL
                     TIYAGTPPYDLSIRLMNYAAEGIVPLANRYVGTNILIQRINITSLIQADGLIMDLNPR
                     VPHVWLITAARRILGRPTMLWGHAWPRSGPNAKSDRVRGWLRTLATGLVAYTESQAEG
                     LRSRHPRKFIVAAPNALYSCREMRFNSESRRFRILYVGRLVQDKKPRILIDAFEKMAR
                     SAPNTLLTFIGDGPERASMQKRIAESPYRDRIEMPGYISEFEKLRPFYDESLVSVSPG
                     YVGLSITQSLAFGVPMLISRDENHSPEIECAVEGFNSRFFETDNPESLAAALLEFVNQ
                     AEAWRQRGPEIASDCARRYSVERMAGGLMQALVGE"
     gene            2693..3919
                     /locus_tag="VFA02_08365"
     CDS             2693..3919
                     /locus_tag="VFA02_08365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU09905.1"
                     /translation="MRIAFVVSNLTYPPEEGLHQQTLLTAQIHLEHGNEVHLMGFCRD
                     PRKLDLQRMKDETGLSFYMPPIPSRLPDVVLGFVNRFIPGVLRSQAVKRLKKEILSGY
                     DVIHFENIAACGLVRKDMADRAVIGLIDPGTLRWKRMFKAHREYKLKLKAFLGILLHN
                     LLESAVAFPGIRIHVVSQEDANYLKARRKKAHVRPIPVAVPIHFDPVSDATEKYFERK
                     TGMIFMDLRQPHLRSSFLWFIQNVYRPVCSEECLCDLLVLGRTSGDPELEQVCLGLPI
                     RFVPWVEDLDGTFAAVDFVIVPDQVGTGIKNRVVQAMAAGKPVVGTPIAFEGIPALNG
                     IHALVVNGPDEMARVLIELCKQSDLRELLGKNARKLIVKQFGKESVQESWREFYSLRA
                     LVTPKTRKTTSTFMVC"
     gene            4292..6532
                     /locus_tag="VFA02_08370"
     CDS             4292..6532
                     /locus_tag="VFA02_08370"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU09906.1"
                     /translation="MNTVPQKELTLQDLLRIFDRRRYIFLGTAAGILGLAILACIFMT
                     RRYETTGTVELQKSEAGGLDLESMMGSEASSASDALSVNLDLQTQTEILQSESLLLKV
                     AKDLNLEQNADFKPRFNPIGAVMGLFSPKGIPDPAGASLEDSPGRRRRVLMVMNSHLK
                     VKVTPGTRLITVSYSNPDPKVSAAVVNHLMQALIDFTFQGKYAAMSQVSDWLAGQLGS
                     LRQSSEGLQEKVVQLQKETGLYGVGGTDLQGKPVVYSPALDRLQQSSAQLEQAQLNRI
                     LKQSVYEVAKTGNAELISQLSGTSLAGASSQGVANSLTLIQNLRAQESTLKAQIAQDA
                     SQFGPNFPRLIQERANLASTEQSLKDEINRIAERARNDFEVAQKAEEGSRKTYETDVQ
                     AAQKLNDKTIEFTLLSKEADESQSLYQDLLRHLKEAGILAGLRSSNLTIIDPARPPAA
                     PATPKVPLLLGLGLGVGLFAGAGLSIAADAIDNKIQGIEDIEEQGLTVLGLLPKIKSA
                     EGQGVPIFPVVQDSAYLEAIRSLRSTVLLSRSGAPPKILLVTSASPGEGKSTTSLNLA
                     FCMAQLEKKVLLMEVDFRRPTLRSRLKLPGTEGISTALSGHTPQVNVSTFDGYPNLHL
                     LPAGPVPPYPAEMISSAMMRSLIARWEQEYDFIILDCPPVLPVTDARLLMEFADTAIL
                     IARPGKTTRVALKRAYQLLAPHAKDQNATSLGVVLNAVSVRSASYYGYYGYYGSKKYE
                     YGQKED"
BASE COUNT         1564 a         1657 c         1819 g         1507 t
ORIGIN      
        1 gcggcattca ggtttgtgac ataggcctga tggtgcttgt catggtggag cttcatggtc
       61 gcttcgtcaa tgtgcggttc gagagcggca tagtcatacg gtaggggtgg gacttcgtgt
      121 gccaaagcat ttcctccttc agactgttgt atgtgtatgg gtcttaaaga aaaccctgtt
      181 tccgattcac atgataaggg atgcgcggaa tctatgtttg ggtgcgtgcc ggtgttgatt
      241 ttgcaagact tgcaaaattc gtacgcaaca ttctgccgga aggatggttc ttatgtagag
      301 caaaattggc gttgggccag ctttgccgtc tttttatgaa gtcatttcat cgggcaacta
      361 tgtctgatgt gtccactcac gcataattat tttttaaggg agtcgaaatg aaacgaggag
      421 catttcacag catttccctg gccgtgctct ctgttttcgc catggaccag acggtgttcg
      481 caaacagcgc accgccgcag gcagcagccg caggctacac catgaacacc ttcagcagta
      541 catttcatgg cgatgtggac atccataata cccgcgcccc tggatttaac tggtatctgg
      601 ccaaattttc aggagcgcgc acaacagagg catcttcgct gcatttcaac agtgatggaa
      661 gcctgaccct gacctccgga ggagatacgg caaacgatgc catcaacagt gccgcggaca
      721 ctggaaaacc cgcaccgaat gactgggtag gcacggcctt cggcggcggc ggatattttg
      781 aagctgaatt taaatttgac ccttcctttg tcatgaagcc gggggcggtt ggttttcctg
      841 cctggtggct tgagccgatt gaacacatca agaggtcggg tggggcgcac tggaatgggc
      901 aggcatccgg gtttgagcat tttacagaag ttgatatatt tgaatacaac caatggaaac
      961 aacattcacc aaatatttat gccggcactg tccatgagtg gtacggtata cataacgtta
     1021 catgtccggg caaagcattc tgccacgcct cgaatgaaaa caacgcaaca acacagtttg
     1081 cgaacaatct gatagtaacg cctccgggca ccgatttcag gaaatatcat aagttcggcc
     1141 tgctctgggt accggcaacc aataccagtc agggctactt acagtattat tttgatggaa
     1201 aacccacaaa cgacaggatt acgtggagtc aatatgatga gcaaaaacac aagcctttca
     1261 tgttgaagac ctcagcgcaa tctataccgt gggtatttgg cgttctcgac cgtcaacacc
     1321 ttgttttgat tcttggcacc ggaaacggcc agcctatgac gataaaagat gttaatgtat
     1381 ggcaaaaaaa ttccgatcag aacttaaagc agtaattttt ctccctgatc cgcgatagac
     1441 tcgcttatct ataaacagcc agaatttccg ggcagctttt ctactttggg aaagtctgcc
     1501 cattcatacc tgataacttc tctgcctctg ttttgtttga tagttaaaag tttttagaac
     1561 actaagtccc agaaggtaca tggaacgaat caggacatcc ctgcgcaaaa agcgagataa
     1621 accgcgccgc tatgtgatag cagttgcttc actgggtgat taccgtgccc gggtagtccg
     1681 cgagatccgg tccaggttag gaagctctct gacaatttat gccggcacac ctccttacga
     1741 cttatctatc cggcttatga attatgcggc tgagggcatc gtgccgctcg ccaatcgcta
     1801 tgtgggaaca aacattctga ttcagcgcat aaatataact tcgctgatac aggcagatgg
     1861 cctgatcatg gacctgaatc cgcgagttcc ccatgtgtgg cttattactg ccgcgcgacg
     1921 cattctgggc agacctacga tgttatgggg acatgcatgg ccaagaagcg ggcccaatgc
     1981 gaaatctgac cgtgttcgcg gctggctgcg cacgctggcc acaggtcttg tggcttatac
     2041 agagagccag gctgagggtc tgcggtcgcg gcatccgcgc aaatttatcg tggccgcccc
     2101 caatgccctt tactcgtgcc gggagatgag gtttaactct gagagccggc ggttccgcat
     2161 tctttatgtg ggccgcctgg tccaggacaa gaagccacgc attctgatag atgcctttga
     2221 gaaaatggcg cgttctgcgc cgaacaccct gctcactttt attggggacg ggcctgagcg
     2281 cgcgtccatg cagaagagaa tcgcggaatc gccatatcgc gaccggattg agatgccagg
     2341 gtatatctca gagtttgaga aactgcgtcc tttctatgat gaatcccttg taagcgtgtc
     2401 tccgggctat gtagggctgt ccatcacgca aagcctggct ttcggcgttc cgatgcttat
     2461 ttcccgggac gagaaccatt cgccggaaat cgagtgcgcg gttgagggat ttaacagcag
     2521 attttttgag acagacaatc ctgaatcgct tgcggcggcg ttgcttgagt ttgtaaatca
     2581 ggctgaagca tggcggcagc gcggcccgga aatcgcttcc gactgcgcgc gacggtactc
     2641 ggttgagcgc atggcgggag ggctgatgca agccctggtg ggagagtaag cgatgcggat
     2701 tgcttttgtc gtgtcgaacc tcacgtatcc gcctgaagag ggactgcacc agcaaacact
     2761 gctgacagcg caaatccacc tggagcacgg caatgaagtg catctgatgg gcttttgcag
     2821 ggacccccgc aaattagatc tgcagcgaat gaaggacgag acggggcttt ctttttatat
     2881 gccaccgatc ccatcccgtc ttccggacgt tgttcttggt tttgtaaacc gcttcattcc
     2941 cggagtgctg cgcagccagg ccgtgaagag gctgaagaaa gaaattctgt caggatatga
     3001 cgtgatccac tttgagaaca tcgcagcgtg cggccttgtg cgcaaagaca tggccgaccg
     3061 cgccgtcatc ggcctgattg atcccggaac attacgctgg aagcgaatgt tcaaggcgca
     3121 tcgcgaatac aaactgaagc tcaaagcatt cctgggcatt cttcttcaca atctgctgga
     3181 gtctgccgtg gcttttccgg ggatacgcat tcatgtggtg tcgcaggaag atgcgaacta
     3241 tctgaaagcg cgcaggaaga aggcacatgt gcggcccatc ccggttgcgg tcccaattca
     3301 ctttgaccct gtcagtgatg cgacagagaa gtactttgaa cggaagacgg gaatgatttt
     3361 tatggacctg cgtcagccgc acctgcgcag ttcctttcta tggttcatac agaatgtgta
     3421 ccggccggtg tgctctgagg aatgcctgtg tgatctttta gtgctgggac gcacaagcgg
     3481 tgatcctgaa ctggagcagg tctgtctggg actgcccatc agattcgtgc cctgggttga
     3541 agacctggat ggcacatttg cggccgttga tttcgtgatt gtgcccgacc aggtggggac
     3601 cggcattaaa aaccgggtgg tccaggcaat ggcggcaggg aaacccgtgg tcgggacccc
     3661 tatcgcattt gaaggcattc ccgctttgaa cggcattcat gctcttgtgg tgaatggccc
     3721 ggacgagatg gcgcgagtac tgatcgagct atgcaagcag tctgatcttc gggaactgct
     3781 cggaaagaat gcgcggaaat tgatcgtgaa acaatttggc aaagagtccg tacaagagtc
     3841 atggcgtgaa ttttacagct tacgggccct ggtgacgcca aaaaccagaa agaccaccag
     3901 cacgtttatg gtttgttaat gcagagaatg gcttggaaat aataactttt tattataaat
     3961 aactattgtt cgtaaggagt aaaaggtcca ttactcttcc ccatgttgtt cgtgttattt
     4021 ctcgctgaaa ggccagattt ggcgaaagaa agggaaagcc ctgaacggac gtagtcacgg
     4081 aaagctcccg cgaacgggcg cactacgcta aaataaagct acctctgata caaactgggt
     4141 ggaccaaaaa gatgaaacag catctcaccc cagatttgtg tatctgcaag ctcaactgat
     4201 tcgggtaact tctctgccgc ggtcgaacaa agacatagct ttctgagaaa agctgaaatt
     4261 tatatctcta tctatctggg agtgtgaacg cttgaatact gttccgcaaa aggaactcac
     4321 cctacaggac ttgctcagga tattcgaccg gcggcgctat atttttctgg gaacggcagc
     4381 agggattctg ggcctggcca tccttgcgtg catttttatg acccgcaggt atgagaccac
     4441 cgggacggta gagctgcaga agtccgaagc aggcggtctt gatcttgaaa gcatgatggg
     4501 aagtgaggcg agcagcgcat cggatgcgct ctcagtcaat cttgatctcc agacccagac
     4561 agagattctc cagtcggagt cacttcttct gaaggtcgca aaagacctga acctggagca
     4621 gaacgctgat ttcaagccgc gctttaatcc aattggcgca gtgatgggcc tgttttctcc
     4681 caaagggatc cccgatcctg ctggcgcaag ccttgaagac tcgcccggac gccggcggcg
     4741 cgtgctgatg gtcatgaaca gccatctgaa ggtgaaggtg acgccgggga cgcgactgat
     4801 tacagtctcg tacagcaatc ctgatccgaa agtgtccgca gcggtcgtaa accatctgat
     4861 gcaggcgctc attgatttca cttttcaggg aaaatatgcc gcgatgagcc aggtttcaga
     4921 ctggctggcc ggccagttgg gttcgctccg tcagagcagt gagggcctcc aggaaaaagt
     4981 ggtccagctc caaaaggaaa ccggattgta cggagtgggc ggcaccgacc ttcagggcaa
     5041 gcctgttgta tacagcccgg cactcgacag attgcagcag tcatcggcgc agcttgagca
     5101 ggcgcaactg aaccgcatcc tcaagcagtc ggtctatgaa gtagcaaaga ccggcaatgc
     5161 ggagttgatc tcgcagttga gcggcacatc gctggccgga gcatccagtc agggagtggc
     5221 caattcgctg acgcttatcc agaacctgcg tgcccaggaa agcacgctga aggcgcagat
     5281 tgcgcaggac gcctcgcagt tcggccccaa ttttccgcga ttgattcagg agcgggcgaa
     5341 cctggccagc actgagcagt cgctcaagga tgaaattaac cgcatcgcag agcgcgcccg
     5401 aaatgatttt gaagttgcgc agaaggccga agaaggttcg cgcaaaactt acgagacgga
     5461 cgtgcaggcc gcacagaaac tgaacgacaa aaccattgag ttcactttgc tgagcaaaga
     5521 ggcggacgaa agccagagcc tctatcaaga cctgctgagg cacctgaaag aggcgggcat
     5581 tcttgcagga ctgcgttctt ccaatctgac cattattgat ccggcacgtc ctccggcggc
     5641 gcccgccacg ccgaaagttc cgctgctcct ggggctgggg cttggtgtgg ggctatttgc
     5701 cggagccggc ctttcgattg ccgcagatgc cattgacaac aagatccagg gcatcgagga
     5761 cattgaagaa caggggctga cggtactcgg tctgttgcct aagatcaaat ccgcggaggg
     5821 gcaaggtgtg ccgattttcc ccgtcgtgca ggattcggcc tatcttgagg ccatccgcag
     5881 ccttcgctcc acggtcctgt tgtcgcgaag cggagcgcct ccgaaaattc tgctggtgac
     5941 gagcgcaagc ccgggtgagg gcaagtccac gacatcactg aacctggcct tctgcatggc
     6001 gcaactggaa aagaaggtgc ttctgatgga agtggacttc cgccgtccca ctctgcgctc
     6061 gcggctgaag ctgccgggca cggaaggcat cagcacagcg ctctctggac atacgccaca
     6121 agtcaatgta tccacattcg acggatatcc caacctgcat ctgcttccgg cagggcctgt
     6181 tccaccttat ccggcggaga tgatcagctc tgcgatgatg cggtccttga tcgcccggtg
     6241 ggagcaggaa tacgacttca tcattctgga ctgcccgcct gtccttccag tgacagatgc
     6301 acggttgctg atggaattcg cggacaccgc cattctgatt gcacggcccg gaaagacaac
     6361 aagagtggcg ctgaagcgag cgtatcagct tcttgctcct catgcgaaag accagaacgc
     6421 gacctccctg ggtgttgtgc tgaatgctgt ttccgtacgg tcggccagct actacggcta
     6481 ctatggttac tacggcagca aaaagtatga atacggccag aaggaagact gatgatgaag
     6541 agactaa
//
LOCUS       DASRBV010000225        30833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_678384, whole genome shotgun sequence.
ACCESSION   DASRBV010000225 DASRBV010000000
VERSION     DASRBV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 30833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30833
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678384"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="argG"
                     /locus_tag="VFA02_08375"
     CDS             complement(<1..992)
                     /gene="argG"
                     /locus_tag="VFA02_08375"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZU09907.1"
                     /translation="MSVILESLPVGQKVGIAFSGGLDTSAALHWMKQKGAIPYAYTAN
                     LGQPDESDYDAIPRKALEYGAEKARLIDCRSQLVREGIAALQSGAFHISTAGVTYFNT
                     TPLGRAVTGTMLVTAMKEDGVNIWGDGSTFKGNDIERFYRYGLLVNPDLQVYKPWLDP
                     LFIEELGGRAEMSAFLQKSGFAYKMSAEKAYSTDSNLLGATHEAKDLEQLSSSIRIVE
                     PIMGTAFWRDDVEIRREEVTVRFEQGVPVALNGKTFAGLVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLFIAYERLITGIHNEDTIEQYRENGRKLGRLL"
     gene            complement(1172..2167)
                     /gene="argF"
                     /locus_tag="VFA02_08380"
     CDS             complement(1172..2167)
                     /gene="argF"
                     /locus_tag="VFA02_08380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZU09908.1"
                     /translation="MASKSLAVLEPEAGLGASLAGRDLCSISDLTPQETAAILELAHD
                     VKANPSAYRHALDAKQMVMFFEKSSLRTRLTFETAIHTMGGNAIFVDQTQSPLGERES
                     LADMARNLERWVSIIVLRTYAHETITEMAQYASVPVINALSDLEHPCQALADFMTLEE
                     RFGTVKDLKFTYVGDGNNVCHSLMLTAAQLGAHCTIGTPKNYAPKADIVAQARAIANV
                     TGSNITVINDPVAAVTGADAVYTDVCTSMGFEHEATKRAPIFKPYQVNETLMSHAASH
                     AVFMHCLPAHRNAEVTDAVLDGPQSVVFEQAENRMHAQKALLLLLLGVGKKGVKK"
     gene            complement(2160..3419)
                     /locus_tag="VFA02_08385"
     CDS             complement(2160..3419)
                     /locus_tag="VFA02_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZU09909.1"
                     /translation="MKLDAIRAAEAKLLLSTYERNPLLFVRGEGVHLMDENGDRYLDL
                     LSGIGVNALGYGHPAIVDTIAEQSKKLVHISNLYFHEGQAELALRLTEKSGMDRAFFC
                     NSGTEAMEAALKLARAHAGQKRGEGRDLGTKILALEHSFHGRTMGSVAATHKEKYREP
                     FAPVMPGVEFVRFNDVADLKAKFSTGVCAIVLEAIQGEGGIRPVSQEFMQTARDLTQS
                     SGALLIVDEIQAGLGRTGKWFAYQHYSIQPDVTTIAKPLAGGLPLGAMLCKEEVAQVI
                     HPGMHGTTFGGGPLACAVAIAVIDAIEKQHLLDRVNEVGSYFRQKLEALKRKHGAITE
                     VRGLGLMLAIEVDSADLAKTVLAEMMKRRILINRTSETVLRFLPPYVIQQQHVDEAIA
                     ALEQIFTEHSPAHAGEHAHTQGGTNVG"
     gene            complement(3492..4274)
                     /gene="argB"
                     /locus_tag="VFA02_08390"
     CDS             complement(3492..4274)
                     /gene="argB"
                     /locus_tag="VFA02_08390"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZU09910.1"
                     /translation="MKFIVKLGGAALENPDLRESCAKAIAELVKDGNQVVLVHGGGVQ
                     LTRTLKQLGKQSEFIAGLRVTDAETRDTALMVLSGQVNKSLVAALGKHGQAAVGLCGG
                     DGLVFRARKKRTAPDLGFVGEIVASDPRWIEAIWKMGAVPVISSIALGFDGEYYNVNA
                     DEMAAACAAACKADALVFLTDVPGVKDANGEVMRWLSIDQIAKLAKDAVISGGMLPKL
                     SACREALLQGVKRVRILPAEAAGVLPDLCSARVNHGTEVMVA"
     gene            complement(4271..5323)
                     /gene="argC"
                     /locus_tag="VFA02_08395"
     CDS             complement(4271..5323)
                     /gene="argC"
                     /locus_tag="VFA02_08395"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZU09911.1"
                     /translation="MTKKIQTAVMGVSGYAGAELARLLLHHPQLKGVPPVFLGRAETA
                     QPLTELHPQLMDNNGSHALKVEPFSWDLLKERGVDVLFLATPHEQSREWAPQALEHGI
                     RVVDLSGAWRLTHAENRAVYKFADEDSAVALRTQTKAVYGMPELHRAEIKDAELVANP
                     GCYSTSVILALKPLVARGWVDLDRGIVSDSKSGVSGAGKAPTAKTHFMHAADNLSAYG
                     VFGHRHTGELLEQLELKPEQITFTPHLLPIPRGILSTIYIALEEKKTPEEIERCFRDF
                     YKDSPLVRVFPQGHLPQIQYSVRTNFCDIGFQLAADGRRCVIVSCLDNLLKGAAGQAV
                     QNLNVMYGWNEAEGLI"
     gene            complement(5320..5790)
                     /locus_tag="VFA02_08400"
     CDS             complement(5320..5790)
                     /locus_tag="VFA02_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HZU09912.1"
                     /translation="MSKTERHNAIRELVATTVISNQEELRRKLVRRGFDVTQATVSRD
                     IHELHLYKGPNGYALPNGNGNGNGHFRMEEEETGLPSVHEVLRSFGLKVKQAQNQLVV
                     ITATGGAQPVALAIDHEDWKEVVGTLAGDDTVLIICPDQKSASYLKARLEEMIG"
     gene            6010..10461
                     /gene="gltB"
                     /locus_tag="VFA02_08405"
     CDS             6010..10461
                     /gene="gltB"
                     /locus_tag="VFA02_08405"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZU09913.1"
                     /translation="MGYIKFSDSPVPAGRNGVSSLVDPRFEHESCGVGFIATLKNSPS
                     HDILDKALIALSRLAHRGAVAADGKSSDGIGICTGIPREFLLSSCSIQLAPEAPFAVG
                     VVFFPPDVAECHTELEQALAAQGCCVLAWRDVPTRPEVLGEIALSTMPVIRQVFITSD
                     NAEQFNRRLYLARKQFERSGAPGYVCSLSSTTMVYKSMCAGRLLPEFYPDLKDPRFIT
                     PFALFHQRYATNVAPSWDRAQPFRMLAHNGEINTVWGNRARLDARAPLLPDELKPLVS
                     PDGSDSTSLDEMLELLAHNGRTIAESVRLLLPPAQEKGNSAFFAYNEDALEPWDGPAA
                     ISFTDGRFVGAALDRNGLRPCRFFVTEDMVVVGSEAGLVDLDPEKVVHSGRLGPGEML
                     VADIEKQKFYENDELLHIFDNAAPQYENLLEETTLDPNTPVPALDSADLLRLQHGFGY
                     TREDVKMILQPMATDGKDAVWSMGDDTPLAPLARTPRPVYAYFRQRFAQVTNPPIDSL
                     REACVVQLHTRLGPWPDLLNKKAPLPGLSLSSPFLSLGQIEALRTRQHGLTDELPLAV
                     LDCVFNPACNLLTALDDLCAKAIDLVRGGAAILLLTDRTCSQTAIPIPMALATGAVHH
                     ALIRAGERTRVGLAVEAGDCRDLHHGAVLLGMGAGAVCPWLALETARASNPEKGEENL
                     LHAFDLGLAKIMSKMGISVVDSYRGAHLFDSIGLSQEVVDKCFFGTPAPLGGIGFTAL
                     ESMVRDLWTASFNENGDSGNGEGITATAVKDLPDYGWVRFRKAEKAEPHGWQPQTVRL
                     LQTVVGTARGATATAAEPGQAWAAFASIAVEKEPAVLRDLLEIKPAGAPLALEQVEPS
                     SNYYKRFIASAMSLGSLSPEAHQTITAAMNMLGGRSNTGEGGEDRAVYNPKGEITLAG
                     VSYPAPLLNNKIKQVASARFGVTAEYLMNAEEIEIKIAQGSKPGEGGQLPGHKVTELI
                     ARLRHAQPGIQLISPPPHHDIYSIEDLAQLIHDLRRVNPNAAIGVKLVSELGVGTVAA
                     GVAKAYADYIVIAGHNGGTGASPLSSIKYAGNPWELGLAEAQQVLIRNGLRGRVRLRT
                     DGGLRTAKDVLYAAMLGADEYAFGTAVLVALGCDMARQCHLNTCPTGIATQRPDLRAK
                     FRGRPENLVRFFEELARDIQVWLAKLGLPSLEAAIGRTDLLEQVRYDGGLDLKPLLAA
                     PVNGSALSWQGKRNLRPQEHPEIDDAWVAPAVAAYKEGRPFEIEATITNENRTLGARI
                     AGELALLQDYGVEPKAPVAFRLKGVAGQSFGAFAVPGMTLTLDGVANDFVGKGLCGGE
                     IILRGQGRAALESERHVILGNVALYGATSGRLFAAGRAGERFAVRNSGALAIVEGVGD
                     HGCEYMTGGVAVILGSTGINFGAGMTGGLAWVFDEDGTFLKEERYHDDFLLAELYDTL
                     DAEAQQSIRDLIALHAEKTGSTRAKRLLSDWERFSGRFIRLTPRPQA"
     gene            complement(10533..11219)
                     /locus_tag="VFA02_08410"
     CDS             complement(10533..11219)
                     /locus_tag="VFA02_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HZU09914.1"
                     /translation="MHPEVLQLFQEEYRVLRPRAPIPPIHVRFYHFAGLNTTIRLRDG
                     ELKVRLSDLLEGAPQPVLRAIAHILLAKLYRKSLDPAHVTRYRRFASSEHVMRQTERV
                     RQMRGRKKISTAKGEHYDLEEVFESLNQRFFHGLMGRPVLTWSEHKAKRLLGHYDAAH
                     NTIMISRVFDRKNTPRYAIEYLVYHEMLHLKHPVKVRGGRRCVHSLEFQEEEKLFPHL
                     EEAKEYLKRL"
     gene            11278..11796
                     /locus_tag="VFA02_08415"
     CDS             11278..11796
                     /locus_tag="VFA02_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZU09915.1"
                     /translation="MRRVIYEVACSLDGYIARLDHSCDWLPNDEQDYGLKDFYARLDT
                     ALIGRKTHGLMVKHGQPAMPGLTNYVFSRNPPSATYEGVQWVSSSPLELIGTLKSKPG
                     KDIWLVGGSNLAQTLFGSRLVDEVSLAIVPVLLGDGIPLFPRFTAETPLKLIEEKAYP
                     GGVARLRYERVS"
     gene            complement(11837..12910)
                     /locus_tag="VFA02_08420"
     CDS             complement(11837..12910)
                     /locus_tag="VFA02_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZU09916.1"
                     /translation="MTETGTPREHGYRMPAELAEHAATWIAWPHNANDWPGKFQAIPW
                     VYTDIVRHLSRVEDVHILVNDAAAEKRAKGILKRSAANLARVHFHQWPTNRVWTRDSG
                     PIFIKNPQGQLAITNWKFNAWAKYDNWQLDNQVPDHVAEELRLPQWTPMIGGHRVVLE
                     GGSIDVNGAGTLITTEECLLSEVQQRNPGISREQLEQVFHDYLGIDQVIWLNRGIAGD
                     DTHGHVDDITRFVNENTIVTVTEPNRDDENHLPLAENLDRLRSARNPDGQPFRIIELP
                     MPGPVIFDGQRLPASYGNFYIANKLVLVPTFNDPNDRRALNILAELFPNREIAGIHCG
                     DFIWGLGALHCMTQQQPLSSTPF"
     gene            complement(12995..13918)
                     /locus_tag="VFA02_08425"
     CDS             complement(12995..13918)
                     /locus_tag="VFA02_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HZU09917.1"
                     /translation="MPSSLNYKIGLVQMSCTPDPDTNLEKAIERVREAARQGANVICL
                     PELFRAQYFCQREDHALFDIAEPVPGPSTERLSAVAREEKAVVVASLFERRAPGLYHN
                     TAVILQKDGSIGGLYRKMHIPDDPLYYEKFYFTPGDLGFKAFDTAVGKIGTLVCWDQW
                     YPEGARITALQGANVLFYPTAIGWHPAEKEQYGTAQYEAWQTIQRAHAIANGVYVGAV
                     NRVGHEQGDVRGNRVEGPGLEFWGGSFIADPFGRIIAKASHDKEEILIGEVDVRTLED
                     VRRNWPFLRDRRIDAYAPIVHRFLDTTGPEK"
     gene            complement(14012..14569)
                     /locus_tag="VFA02_08430"
     CDS             complement(14012..14569)
                     /locus_tag="VFA02_08430"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HZU09918.1"
                     /translation="MGKSMVPRRLFFTKGVGKHKERLTSFELALRDAGIAAQNLVRVS
                     SIFPPQAKLIPRKEGLKYLHPGEVVFAVVAENSTREPHRLIASSIGVAIPSDRNTYGY
                     LSEHHSFGETEEQAGEYAEELAAEMLATTLNLEFDPDKSWDEKKEIYRISNKIVRTAN
                     VTQSAIGDKHGRWTTTIAAAILIFE"
     gene            14797..15486
                     /locus_tag="VFA02_08435"
     CDS             14797..15486
                     /locus_tag="VFA02_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZU09919.1"
                     /translation="MKRVLAVMASICLTLPLMAQEKERQRIKDSAQVLSEILATPDKG
                     IPQDLLDKADCVIIFPSVKKGAFIVGGSYGRGVVTCRSGVDFSGPWSAPAMFALEGAS
                     VGFQIGGQATDFVILVMNQGGANSIMSSKVKLGADASVAAGPVGRNTSAETDIVLKAQ
                     MLSWSRARGVFAGISLAGSTLRSDDGANKNLYGKDLTAKEIVREGKVKPPVSARRLLA
                     ILTKASPHHKA"
     gene            complement(15531..16286)
                     /locus_tag="VFA02_08440"
     CDS             complement(15531..16286)
                     /locus_tag="VFA02_08440"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09920.1"
                     /translation="MHWKLALAIGSCALLGLRHGFDYDHLAAISDITAVQRSRFEGIR
                     LGILYALGHAFTVLTLGALVILLHLSLPARLDAWAERIVGFTLIVLGLLVVANLVRKR
                     QHSHSHQAMESRWALLINAVRHAAWRVRRLIEKDAPQPKPFTWNYSNGAVFCIGILHG
                     LGAETPTQLMLFLLTASLGGTMLGLLGLLAFGLGLVIMNAIMTAALSGIFGTGRAHPN
                     FYRMIAAAGAVYSLAIGFIFLLGASERLPSISG"
     gene            16378..17439
                     /gene="queA"
                     /locus_tag="VFA02_08445"
     CDS             16378..17439
                     /gene="queA"
                     /locus_tag="VFA02_08445"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZU09921.1"
                     /translation="MLVSDFDYDLPEDRIAQRPLADRAGSRMLTLARATGDFRDRMFR
                     DFPSLLREGDVLVLNNSRVIPARLFGHRAGLHTQSQHTTTGRVQVLLTEQISEWEWRV
                     LARPGRKVLKGEVLTFGDNDLRAEVVEHGDFGERVLKFEPVADFYGILEKLGHMPLPP
                     YIHREDEDADRERYQTVYAQQRGSVAAPTAGLHFTPEILEEIRKRGVEIVYITLHVGL
                     GTFQPVRVEKVEDIHLHTERYTLSDETASAINRARNEGRRIVAAGTTTVRTLEHCALK
                     AGEKPLGPHSGSTDIFISPGFEFRIVNALLTNFHLPKSTLLMLVSAFGGKEHVLRAYN
                     HAVEAGYRFFSYGDCMFLS"
     gene            17478..21887
                     /locus_tag="VFA02_08450"
     CDS             17478..21887
                     /locus_tag="VFA02_08450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZU09922.1"
                     /translation="MQSVSVPESLAWAHPVVQEWFVHRFGTPTEPQEQGWPCILAGKP
                     TLISAPTGSGKTLAAFLICIDQLIRKALEGRLGPETEVVYVSPLKALSNDVQKNLETP
                     LREIQQLALECGHLAPQIRTAVRTGDTLPAERQKMLKQPPHILVTTPESLYIMLTAEK
                     SRNNLRHVRTVIVDEIHAMVDDKRGAHLALTLERLDALVRGENRLSVGEWLTGHNEAP
                     QRIGLSATQNPIELVAEFLQGGCRQRVEIVQVGQRRELDLAIEVPDEELGAVANNSIW
                     DGIYKRLAELALEHRSTLVFVNTRRMVERIAFRMGELLGEENVAAHHGSLSRKLRLEA
                     ERKLKQGEIRLLVATASLELGIDIGNVDLVCQINSPRAIAVAMQRVGRAGHWRGAIPK
                     GRFFPTTRDDLIEQAATIRAMRAGVLDKLEVPPKPLDVLMQQIVAACAAEPWEEDALF
                     QVLRRAQPYHSLTRDEFEEVLCLLTDGIEASRGRYGSYLLRDRVHGRIQARRGSRTIA
                     VSNAGTIPDTALYAVVAEPEGVQIATLDEDFAVESSAGDIILLGNTSWRVERVESAGR
                     VFVSDAQGQPPTVPFWRGEAPQRTIELSQFVSDLREEIDRRTKNVLPGYIGQSNPEIA
                     EAVTWLKQECGVCDSGAEQIIGYIAAGRSVLGTVPSLTNIVAERFFDEGGGMQLVIHA
                     PFGGRINKAWGLALRKRFCRGFNFELQAAATDNGLNISLAEQHSFPLSDVFHFLTEQT
                     VPELLEQASLASPIFKARWKWAAGRSLQLLRYKNGKRIPPQIQRTRGDDLLASVFPQV
                     AACFENIEGDIVIPDHPLIREVMKDVLGEAMDLEGLQRVLADMHSGKIRCVAVDTPTP
                     SHFAHEMLNANPYAYLDDAPLEERRARAVNMRGILPDNLLGEAGRLSPAAIASVREEV
                     WPDVRDEHEFHDLLCSLVIVPAEILLEPQARNWDIFFARLEEQARVCIAQAGGREYLC
                     AVELMEYLKQVFPDVQFASSSAYVSDPATHEDALRKAVQGWMSVLGPASSRTLSEKLG
                     INAGEVWKQMLHLEMTGTVLRGVFEGQGRPEGALADEDVEWCERRLLQRIHKRTLVAL
                     RKQVEPVSPSTFMQWLLRWQHVAPRTQLSGEAGLLEALRKLEGFEAPAIEWERSILPL
                     RVAGYDPRWLDALCLSGVVGWGRISPHPAFNSIESGGPRRVVPTSMAPITFFIREEAL
                     WMDLCLSQRHIPEPCLNACLSEVASKVRNALSSQGAMFAGDLVRRLSVPSAEVDIALW
                     ELVAAGLITADGFDSLRMLIDPRRNLGRKTKQAAGRWSLFRSTDEQTPTRREREIESA
                     CRMLLRRYGVLFRDLLERETTIPRWRDLLGILRRMEARGEVRGGRFVSGFNGEQFALP
                     EALDSLREARRSGTQFAPVSVAAADPLNLIGIAVPGERVAAVAGNKVMLDEAMLFPTL
                     SQKPGKDSAPKSNGVAVSPLLLTESKYEQPPTA"
     gene            21865..22047
                     /locus_tag="VFA02_08455"
     CDS             21865..22047
                     /locus_tag="VFA02_08455"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HZU09923.1"
                     /translation="MSSPPQPDQPQPLIEGVDYYMDGPYLVFTEAYHLKRGHCCGSGC
                     RHCPWKNTESEKSSNA"
     gene            complement(22120..24366)
                     /locus_tag="VFA02_08460"
     CDS             complement(22120..24366)
                     /locus_tag="VFA02_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU09924.1"
                     /translation="MTQSKRFVILGEAKDLLLESIHHTAELLLRRRTCATALLLAGTI
                     LLPLYAQQDNQQSQSFTLKVNSDLVLTNVVVRDKKTGQIVKGLTKNDFTITENGKPQQ
                     IISFDFESVDEAAALNEATISGRAGNIVLGKTGVITGDALRDHRLVILFFDLTSMQPE
                     DVERAQDAARNYINKQMQPADVVAVVSLDTSISVDQDFTQNKQLLLNAINSYSGDQTQ
                     GYAPGATSTTNQTEDTSAYTPDESEYNDINTDRELYAIASIAKSLNYINQKKSLVYFS
                     GGISRDGIENQASLRSAINASVRSNMSIYSVDARGLQAISPLGDASTGSLRGTSAYNG
                     AALQNNLDANFNSQEVMANLSSDTGGKAFFDSNDFAPAFQQMQQDTSAYYVIGYRSTD
                     PRRDGAYRRLTVKVDRPDVKLEYRHGYYAPADFRHSNKEDRERELEEQLASDLPATDV
                     AVYLQALYFRMDENRYYIPISILVPGSQIPFVKGGDRDKATLDIIGEVKDAAGRRIGD
                     ARDTVKLAVDQSQQVRQKNIQYSTSFTLPAGKYHLKFVVRENETGRMGSFETDINVPD
                     LRKAPVRLSSVVLASQRIPANKKSDSPLVHDGQELVPNLAHVFRQNQHLYFLYEVYNP
                     AKAAGQGQEITTSLAKTKNAKDAPPAPVRVMTSIEFLSNGLKVFETPMVQATQINEPS
                     RDAVAFQFDVPLTDLKPGLYTCQINVIDDAGGSFTFPRTALLIREGANTAQPAGTSSS
                     GSGSPGGR"
     gene            complement(24380..27727)
                     /locus_tag="VFA02_08465"
     CDS             complement(24380..27727)
                     /locus_tag="VFA02_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09925.1"
                     /translation="MLRRLLPICLLLVFSTYCVAQTQGPSTTGGTVHGSVKSGNVPLP
                     GVSITATNTLTGKKYSTVTDVNGAYSLNIPQNGRYVVRTDFAAFATETKEVLFNAASS
                     QNQQADFALKLASRAQQQEGTTQAAQALQTLRGLQNLGLVNAAAGLIQAGSGNAASDT
                     SLPALANNSDFSNESVAVSGQSGSTNPFAGMDIGQMRENMENMQFERSLSQTPGSQSK
                     GGGGGEGIYIGGPGGGPPPPPPGGGGGAVFIGGPRGRGNFRKFNPSQPHGAFFWNGGN
                     SALNAEPFSVRGAPLNQPAYNSNHFGLTFVGAPYIPKLLEHDTKDFLFFTLAGNRTSS
                     PFTQYGTVPTAAERTGDLSTLTNQEGQPITIYDPQTGQPFPNNTIPAQRIAPQATALL
                     NYIPLPNLSGRAQNYQRITTNQNNTTNIGLRYMRSFGSSAGSPIMGMIRQFMGQGGNS
                     WHQSINGNFNYSHTAADELNIFPDLGGKEQMHQYSVQAGYSLSKGRLVNNLNVGWNRS
                     NTQLTNYFTNTTDIARQLGINIFNGAAVSPLNYGLPNLVLNQFTGFNEQQPNFHINQT
                     ISVSESSSWIHKKNNFRFGADFRRVHLDMIGATSNSTGTFYFTGLFTEAPGTIPDTSG
                     ALATSGSSLADLLLGLPQQTALQAPYEKAYLRENVFDAYFQDSWQALPNFTVLAGLRY
                     EYFSPYSEKNDRIVNLDVGNNFSSVVPVYPNGTGPYSGKYPRTLIEPERLNFSPRLGF
                     AWRAVKNTVVRAGYGINFANGQYVKFVQNFAFQPPFADVQTNEVTSGASITLANGFPG
                     PQTVGNYAVNKNYRLPYVQVWNIDVQRTFPLGIVMNVGYNGSKGTRLDIVSAPGRTAT
                     ETLSGYYYDWEDSIAFSNYNALAVRLRKRMQNGIAVGATYTYSHSIDNASSIGGNGGT
                     GLVVAQNWQNLLAEESNSSFDVRHNLKGDFVYELPFGPDTHMLTNGNWLSHTLANVSL
                     SGTFQFATGEPLTPAYAAAVEDVARGSAGSLRPDRVQGVSLTQGGGSLRNWFNRAAFT
                     QPAGTYGTASRYSIPGPGTVSFDASLSKSIRFGDFRNLELRATANNVFNTVQYSGVDT
                     TLGSATYGQVTSAATMRQFTFMGRYRF"
     gene            complement(27753..28412)
                     /locus_tag="VFA02_08470"
     CDS             complement(27753..28412)
                     /locus_tag="VFA02_08470"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HZU09926.1"
                     /translation="MKIRTLAFLVLLGLLPSAYAWQQGGQWGAPFGRGVLGTVTAISG
                     NEFTVKNEQGETWKVETGPNTRFMKDRQPAAASDVHVGDVIMAGGELDEQAHTVGAAF
                     LGVLGPEAVQRMKQMQADFGKTWLAGKITAINDLTLTVQRPDKKTQTITVNENTSFQK
                     HRESITLADIKVGDMVTARGALQNGNFVASVLNVMEPRRPGQGSAPASGNGPPSPQQP
                     Q"
     gene            28618..29883
                     /gene="egtB"
                     /locus_tag="VFA02_08475"
     CDS             28618..29883
                     /gene="egtB"
                     /locus_tag="VFA02_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZU09927.1"
                     /translation="MSQAEAVCQSLALAFCSVRSRSELLCEPLTPEDMMVQSCPEASP
                     AKWHLAHTTWFFETFILSEFCRDYRPFHPDFLWLFNSYYNAVSDQPEKKLRAVFSRPS
                     LTEILDYRHSVNRAMETLLQGQPSPEIEQRVVLGINHEQQHQELLLTDIKHAFWTNPL
                     HPAYLDLRFEKSAAGDADARPQWCEYPGGLAEIGHQGDGFCFDNELPRHRVFLEPFAL
                     ASRLVTCAEYLAFMEDGGYERPNLWLSEGWQTVQSQGWRAPLYWHKEGDEWSIFTLRG
                     NVRLSDVANTPVCHVSYFEADAYARWAGKRLPTEAEWEVAASPQDVQGNMLESGALHP
                     AVANAGHRGMEQTFGDVWEWTASAYLGYPGFHALPGALGEYNGKFMSGQMVLRGGSCV
                     TPQTHIRASYRNFFAPATRWQFSGIRLAN"
     gene            29905..>30833
                     /gene="egtD"
                     /locus_tag="VFA02_08480"
     CDS             29905..>30833
                     /gene="egtD"
                     /locus_tag="VFA02_08480"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZU09928.1"
                     /translation="MNALSAVPHLSPDIFGPVGAEVYAGLTSSPKRLSPWLFYDGRGS
                     QLFEQITELPEYYLTRTERSILQAHADEMVTAAANCDRISVIELGAGTATKTGLLLEA
                     AVRRQKTVAYYPIDVSETPLAEAKRRLEAEIKGVTVVPRVADYTAGLGQIATAGERRL
                     VLYIGSSIGNFEPSDGLQLLRKIRESLTAGDKLLLGVDMAKDARVLLAAYDDAAGVTA
                     EFNKNILERINRELGANFDLTRFRHRALWNPEHSRIEMHLESLVEQQVTISSLDLEIS
                     LECGETIHTENSYKFHDEDVIEMLERAGFHLSD"
BASE COUNT         6465 a         8582 c         8925 g         6861 t
ORIGIN      
        1 agcaggcggc ccagcttgcg gccgttttcg cggtactgct cgatggtgtc ttcattatga
       61 atgccggtga tgaggcgctc ataggcgatg aagagcaggg ccatacccgg tgcctcataa
      121 atgccgcggc tcttggcttc aataatgcgg ttctcaatct ggtcgctcat gcccaggccg
      181 tgacgtccgc caatgcggtt ggcttcgagc atcagctcca ctaggccagc aaaggtcttg
      241 ccattcagtg cgacagggac gccttgttca aagcggaccg tcacttcctc gcgcctgatc
      301 tccacatcgt cgcgccagaa ggccgttccc ataatcggct cgacgatgcg gatgctgctg
      361 gaaagctgct cgaggtcctt ggcctcatgt gtggcgccga gcaggttgga atcggtcgag
      421 taggccttct cagcggacat cttgtaggcg aagccagact tctgcaggaa tgcggacatt
      481 tcagcacgcc cgccaagttc ttcaatgaag agcgggtcaa gccagggctt atagacctgc
      541 aaatccgggt tgacgagcag cccatagcga tagaagcgct caatgtcatt gcctttaaat
      601 gtcgagccat cgccccagat gttgacgcca tcctccttca tggctgtcac cagcatggtg
      661 ccggtaacag cgcgtcccag aggcgtagtg ttgaagtaag tgacgcccgc agtcgagatg
      721 tgaaaggcgc cagactgcaa tgcggcaatg ccttcgcgca caagctggct gcggcaatca
      781 atcagccgcg ccttttcggc gccatactcc agcgccttgc gggggatagc atcgtagtcg
      841 ctttcgtccg gctgacccag attggccgtg taggcgtaag ggatggcgcc tttctgcttc
      901 atccagtgaa gcgcggcgct ggtgtccaag ccgccggaaa aggcgatacc gactttctgc
      961 ccaaccggca gactttcaag aattacagac atttcgtaca aaacctcaaa ttttatgcgt
     1021 tcgctgggtg ccccacatcc cgaagagatg tgggatttca attctgacaa ggactctggg
     1081 cgccgcattc tttacgcagt aaagagtggg atgatatcca ctttcccgtt gcgtgcgccc
     1141 catatgcctg ctgttgtgcc gtaggagaga gctacttctt cacacccttc tttcctacgc
     1201 ccaacagcaa aagcagcagc gccttctgcg cgtgcatccg gttttccgcc tgttcaaaca
     1261 caaccgactg cgggccatcg agcaccgcat ccgtcacctc agcattgcgg tgcgcgggca
     1321 agcagtgcat aaacacagca tgcgaagcgg cgtgcgacat cagcgtttca ttcacctgat
     1381 acggcttgaa gatgggtgcg cgcttggtcg cttcgtgttc aaagcccatg ctggtgcaga
     1441 cgtcggtgta gactgcgtcc gcccctgtga ccgcggccac gggatcgttg atgacggtga
     1501 tattgctgcc agtgacgtta gcgatggcgc gcgcctgcgc aacgatgtcc gcctttggcg
     1561 cgtagttctt cggagtaccg atggtgcagt gggcgccgag ttgcgcggcc gtcagcataa
     1621 gcgaatgaca aacgttgttg ccatccccta cataggtgaa cttcaggtct ttgactgtcc
     1681 cgaaacgctc ctcgagcgtc atgaaatcgg ccaaagcctg gcatggatgc tcgaggtcgc
     1741 tcaacgcatt gatgaccggg acgctcgcat actgcgccat ctcggtaatg gtttcatgcg
     1801 cgtaggtgcg cagaacaatg atcgagaccc agcgctccag attgcgcgcc atgtccgcca
     1861 gcgattcgcg ctcacccagc ggcgactgcg tctggtctac aaagatggcg ttgcctccca
     1921 tcgtatggat ggccgtctcg aaggtcaggc gcgtgcgcag agatgatttc tcgaagaaca
     1981 tcaccatctg ctttgcatcg agcgcgtgcc ggtaagcaga tggatttgcc ttcacgtcgt
     2041 gcgccagttc cagaatggct gcggtttcct ggggcgtgag gtcagaaatt gaacaaaggt
     2101 cgcgccctgc gagcgacgcg ccaaggccag cttccggttc aaggactgca agtgatttgc
     2161 tagccaacgt tggttcctcc ttgggtgtga gcgtgctcgc cggcatgggc agggctgtgc
     2221 tcagtgaaaa tctgctccag tgcagcaatg gcttcgtcta catgctgctg ctgaatcacg
     2281 taaggcggca ggaagcgcag caccgtctcg ctggtacgat tgatcaggat gcggcgcttc
     2341 atcatttctg ccagaacagt tttcgccaga tcggcagagt caacttcgat ggccagcatc
     2401 aggccaagcc cacggacttc ggtaatcgcg ccgtgcttcc gctttaaggc ttcgagcttc
     2461 tgccggaagt agctgcctac ttcgttgacg cggtcgagaa gatgctgctt ctcaatagcg
     2521 tctatcacgg cgatggcgac agcgcaggcc agagggcctc cgccaaaggt ggtgccgtgc
     2581 attcccggat ggatgacctg cgcaacttct tctttgcaca gcatcgcgcc cagtggcagt
     2641 ccgcccgcaa gcggtttggc gatggtagtc acatccggct gaatagagta atgctgatag
     2701 gcaaaccact ttcctgtacg gcccagtcca gcctgaattt catccacgat gagcagtgct
     2761 ccgctggatt gtgtcagatc gcgcgcggtc tgcataaatt cctgcgagac aggacggatg
     2821 ccgccctcgc cctgaatggc ctcaagcaca atggcgcaga cgccggtcga gaattttgcc
     2881 ttcaggtctg cgacatcgtt gaatcggacg aactccacgc ccggcatgac cggagcaaat
     2941 ggctcgcggt atttttcctt gtgggtcgct gcgacagacc ccatcgtgcg cccgtgaaag
     3001 ctgtgctcca gcgccagaat cttcgtgccc agatccctgc cttcgccgcg cttttggcct
     3061 gcgtgcgccc gcgccagctt cagcgcggcc tccattgcct ctgttccgct gttgcagaaa
     3121 aaggcgcggt ccattccgct cttttccgtc agccgcaggg ccagttcggc ctgcccttcg
     3181 tggaagtaga ggttcgagat atggacaagc tttttgctct gctcagcgat ggtgtccaca
     3241 atcgccgggt ggccgtaccc cagcgcgttg acgccgatgc cgctcagcag gtcgagataa
     3301 cggtcgccgt tctcgtccat cagatggacg ccttctccac gtacaaagag caggggattg
     3361 cgctcatagg tagaaagcag cagttttgcc tctgctgcgc gaattgcatc gagtttcatc
     3421 gttgagaaat ctccagaaat caaaaccgct aaaattcttc actgcgcctg gttgagaatg
     3481 acaatcatgg ctcaggccac catcacctca gtcccatgat tgacgcgcgc gctgcataaa
     3541 tcgggcagga ccccggccgc ttccgcgggc agaatccgca cccgcttcac cccctggagc
     3601 agcgcttcgc ggcaggcgct gagcttcggc agcattccgc cgcttatcac cgcgtctttg
     3661 gcgagcttgg caatctggtc tattgagagc cagcgcatca cttcgccatt ggcgtccttc
     3721 acgccgggaa catcggtaag aaagacgagc gcatcggcct tacaggcagc agcgcaggcc
     3781 gcggccattt catcggcgtt cacgttgtaa tactcgccat cgaagccgag cgcgatgctt
     3841 gaaatcacgg gaacagcgcc catcttccag atggcctcaa tccagcgcgg gtctgaggcg
     3901 acaatctcgc ccacaaagcc gaggtcaggc gcggtgcgct ttttgcgcgc gcgaaagacc
     3961 aatccatcgc cgccacagag gcccacagca gcttgtccgt gcttgcccag cgcggccacc
     4021 agtgatttgt tgacctgtcc gctcaggacc atcagcgccg tgtcgcgcgt ttctgcatcg
     4081 gtcacgcgta gtccggcaat gaactcactc tgtttgccaa gctgcttgag cgtgcgggtc
     4141 aactgcacgc cgcctccgtg caccagcacc acctggttgc cgtccttcac cagttcggcg
     4201 atggccttgg cgcagctctc gcgcaggtcg ggattctcca gcgccgcgcc gccgagtttt
     4261 acgatgaact tcatatcaac ccctccgctt cattccatcc atacattaca ttcaggtttt
     4321 gtaccgcctg cccggcagca cctttgagca ggttgtccaa acaggagacg atgacgcatc
     4381 gtcgtccatc agcggcgagt tgaaaaccta tgtcgcagaa gttcgtgcgc accgagtact
     4441 gtatctgcgg cagatgcccc tgaggaaaga cacgcaccag cgggctgtcc ttataaaaat
     4501 cgcggaagca gcgctcaatt tcttccggcg tcttcttctc ttccaacgca atatagattg
     4561 tcgaaagaat gccgcgcgga atcggcagca ggtgtggcgt aaaggtaatc tgctccggct
     4621 tcagctcaag ctgctcgagc agctcgcccg tgtgccggtg accaaagacg ccgtaagcag
     4681 aaagattgtc cgccgcatgc ataaaatgcg tcttcgccgt cggggccttg ccagcaccgg
     4741 aaacgccgga cttcgaatcg ctcacaatac cacggtccag gtctacccat cccctggcca
     4801 ccagcggctt cagcgccaaa atgacggagg tcgagtagca gccaggattg gccaccagct
     4861 cggcatcttt gatctcagcg cgatgcagct ccggcattcc gtagacagct ttggtctgcg
     4921 tccggagagc gaccgctgaa tcctcgtccg caaatttata tacagcgcgg ttctccgcat
     4981 gggtcagccg ccatgctccg ctcaggtcca cgacgcggat gccatgttcg agcgcctgcg
     5041 gcgcccactc gcgtgactgt tcatgcggag tagccagaaa cagcacatcc acgccgcgct
     5101 ctttcagcag atcccatgag aacggctcga ccttcagggc atgggaacca ttgttgtcca
     5161 tcaactgcgg atgcagttcc gtcaatggct gcgcagtttc ggcacggccc agaaagaccg
     5221 gtggtacgcc cttcagttgt gggtggtgca gcagaaggcg cgccagctct gctccggcgt
     5281 atccggaaac tcccatcacc gcggtttgta ttttctttgt catccaatca tctcttccag
     5341 acgcgccttc aaatatgaag cgcttttctg gtccgggcaa ataatcagga ccgtgtcatc
     5401 accagccagc gtgccgacta cttccttcca gtcttcgtga tcaatggcca gtgcgacagg
     5461 ctgcgcgcca ccggttgctg taatgacaac caactggttc tgcgcctgct ttaccttcaa
     5521 cccaaagctt cgcagcactt catgaacaga aggcaaaccg gtctcttctt cttccatgcg
     5581 gaagtgccca ttgccatttc catttccgtt gggaagggca tagccattcg gccccttgta
     5641 caagtgcagt tcgtggatgt cgcgcgagac agtcgcctgc gtcacatcaa atccgcggcg
     5701 aacgagcttg cggcgcagct cctcctggtt ggagatcacc gttgtggcca ccagctcccg
     5761 gatcgcgttg tgtctctcag tcttgctcaa aagtaagaaa aacgcggctg gagcgccgcg
     5821 cctcctcctt gcataaaaat acaaatcaat tgtataaata tgcaaagcac ttttgtcaat
     5881 atcgtttcga taaaagtttt ttttcgcacg cataaggaaa accgctgcac acgcgcgtca
     5941 gacgcagatt cgttgaattt ccgttaaatt ttttgctgct tgccagcaga aagcgaagta
     6001 tagttagcta tgggctacat aaaattctct gattcgccgg taccggcggg acgcaatggt
     6061 gtctcttccc ttgttgaccc ccgctttgag catgaatcct gcggagtagg cttcatcgcc
     6121 acactgaaga acagcccctc gcatgacatc ctggacaagg cgctcattgc gctctcacgt
     6181 cttgcgcacc gcggcgctgt ggctgctgat ggcaaatcca gcgacggcat cggcatctgt
     6241 accggcattc cgcgcgaatt cctgctttcg tcctgtagca tccagcttgc tccggaagcg
     6301 cctttcgccg tgggtgtggt cttctttccg cccgacgtag ccgagtgcca cacggaactc
     6361 gagcaggcgc ttgctgcgca gggttgttgt gtgcttgcct ggcgtgatgt gcccacccgc
     6421 cccgaagtcc tcggagaaat cgccctctcc accatgcctg tcatccggca ggtgttcatt
     6481 acctcggaca atgcagagca gttcaatcgc cgcctttatc ttgcgcgcaa acagtttgag
     6541 cgcagcggcg cacctgggta cgtctgctcg ctctcgtcta ccacgatggt ctacaagagc
     6601 atgtgcgccg gacgtctgct gcctgagttt tatcctgacc tcaaagaccc acgctttatt
     6661 acaccctttg cgctctttca tcagcgttat gccaccaacg ttgccccttc atgggaccgc
     6721 gcgcagccgt tccggatgct ggcgcacaac ggcgaaatca atacggtctg gggcaaccgc
     6781 gccagactcg atgcccgcgc tcctcttctt cctgatgaac tgaagcccct cgtcagtcct
     6841 gatggttctg actcgaccag tctcgacgaa atgctcgagc tgctggcaca caacggccgc
     6901 accattgccg agtccgtgcg tctgttgctg cctcctgcgc aggaaaaggg gaactccgcc
     6961 ttctttgcct ataacgaaga tgccctcgag ccatgggatg gccccgccgc catctccttc
     7021 accgatggcc gctttgtcgg cgctgcgctc gaccgcaacg gtctgcgtcc ctgccgcttc
     7081 ttcgttaccg aagacatggt cgttgtcggc tctgaagctg gcctcgttga tctcgacccg
     7141 gaaaaggtcg tccacagcgg acgccttggc cccggcgaga tgctcgtcgc cgacatcgaa
     7201 aagcagaagt tctatgagaa cgacgaactt ctgcacattt tcgacaacgc cgcgccgcag
     7261 tacgagaacc ttctcgaaga gactacgctc gacccgaaca ctccggttcc tgcgctcgat
     7321 tccgccgacc ttctgcgcct gcaacacggc tttggataca cgcgcgaaga tgtgaagatg
     7381 atcctgcagc cgatggccac cgatggcaag gatgccgtct ggtctatggg cgatgacacg
     7441 ccgcttgcgc cactggcccg cacgccgcgc cctgtgtacg catatttccg gcagcgtttt
     7501 gcacaggtca ccaatccccc gattgattcg ctgcgtgaag cttgtgttgt gcagttgcat
     7561 acgcgtctcg gcccctggcc tgatcttctg aataaaaaag cgccgctgcc cggactctct
     7621 ctgtcttcgc cgttcctctc gctcggccag atcgaggccc tgcgtacgcg ccagcatggc
     7681 ctgacggacg agctgcctct cgccgtgctt gattgcgttt tcaatccggc atgcaacctg
     7741 ctcacagcgt tggacgatct ctgtgccaag gccattgacc tcgtacgcgg cggcgcagcc
     7801 attcttctgc tcactgaccg cacgtgcagc cagaccgcga ttcccattcc catggccctg
     7861 gccacaggcg cagtgcatca cgcgctcatt cgcgcgggag agcgcacccg cgtcgggctt
     7921 gccgtggaag caggcgactg ccgcgacctt catcacgggg ccgtattgct gggcatgggc
     7981 gcaggcgcag tctgtccgtg gctggccctg gaaacagcac gtgcctcgaa tccggaaaaa
     8041 ggcgaagaaa atctgttgca cgccttcgat ctcggcctcg caaagatcat gtcgaagatg
     8101 ggcatctcag tggtggacag ctaccgcggc gctcatctct tcgactccat cggcctcagc
     8161 caggaagtcg tagacaagtg cttcttcgga acgccagcgc cgctcggagg catcggcttt
     8221 actgcgcttg aaagcatggt gcgcgacctt tggacggcca gctttaacga aaatggcgac
     8281 tccggaaacg gtgaaggcat cactgccacc gctgtcaaag acctgcccga ttatggatgg
     8341 gtgcgcttcc gcaaggctga aaaagccgag ccgcacggct ggcaacccca gaccgtgcgc
     8401 ctgctgcaaa ccgttgtcgg cacggcgcgc ggagcaactg ctaccgcggc agaaccaggc
     8461 caggcatggg cagcttttgc cagcatagct gtcgaaaaag agccagcggt actgcgcgac
     8521 ctgctcgaaa tcaagccagc aggagcgccg cttgctctgg agcaggttga gccgtcctcg
     8581 aactactaca agcgcttcat cgccagcgcc atgtcactcg gctcgctcag tcccgaggcg
     8641 caccaaacca tcaccgccgc catgaacatg ctcggcggcc gctcgaacac gggtgagggc
     8701 ggagaagacc gcgctgtcta caatccgaag ggcgaaatca ccctggccgg agtgtcttac
     8761 ccggcacctt tgctcaataa caagatcaag caggtggcct cggcccgctt cggtgtcacg
     8821 gctgaatatc tgatgaatgc agaggagatt gagatcaaga tcgcgcaagg ctcaaagcca
     8881 ggcgaaggcg ggcagctccc cggccacaag gtcaccgagc tgattgcccg tctgcgtcat
     8941 gcgcaacctg gcatccagct catcagcccg ccgccacacc acgacattta ttcgattgag
     9001 gacctcgcac agctcattca cgatctgcgc cgtgtcaatc caaatgcggc cattggtgtg
     9061 aagctcgtct ctgagctggg cgtgggcacc gtggctgcgg gcgttgccaa ggcctatgct
     9121 gactacatcg ttatcgccgg acacaacggt ggtaccggag catcgccgct atcgagcatc
     9181 aagtatgccg gcaatccgtg ggagcttgga cttgccgaag cgcagcaggt gctcatccgc
     9241 aacggcctac gcggacgcgt gcgtctgcgt actgacggcg gcctgcgcac ggcaaaagac
     9301 gttctctacg ccgccatgct gggcgcggat gaatacgcct ttggcaccgc agttctcgtt
     9361 gcgctcggct gcgatatggc ccgtcaatgc cacctcaaca cctgtccgac gggaatcgcc
     9421 acacagcgcc ctgacctgcg cgccaagttc cgcgggcggc cagaaaacct ggtcaggttt
     9481 tttgaagaac tggcccgcga catccaggtc tggctcgcca agctcggatt gccctcgctc
     9541 gaagccgcca ttggccgcac cgaccttctc gaacaggtgc gctatgatgg cggactggat
     9601 ctgaagcctc tgctggccgc tccggtcaac ggttctgccc tttcatggca gggcaagcgc
     9661 aatctgcgcc cgcaggagca tccggaaata gatgacgcat gggtcgcgcc cgctgtcgcc
     9721 gcttacaagg aaggcaggcc ctttgaaatc gaagccacca tcaccaacga gaaccgcacg
     9781 ctcggcgcgc gcatcgccgg tgaactggcc ctgctacagg actacggcgt cgagccgaag
     9841 gccccggtgg ccttccgcct caagggcgtg gccggacagt ccttcggggc ctttgctgtc
     9901 cccggaatga cgcttaccct cgacggggtg gccaacgact ttgttgggaa ggggctctgt
     9961 ggcggtgaaa tcatccttcg cggacagggc cgcgctgcgc tcgaaagcga acgccacgtc
    10021 atcctgggca acgtcgccct ctacggtgca accagcggac gcctctttgc cgcaggacgc
    10081 gcaggtgagc gttttgctgt ccgcaactca ggtgctttgg ccatcgtaga aggagtcggt
    10141 gaccacggct gcgaatacat gaccggaggt gttgccgtca tcctcggcag caccggaatc
    10201 aacttcggcg ctggcatgac cggcggtctg gcatgggtct tcgatgagga cggcaccttc
    10261 ctgaaggagg aacgctacca cgatgacttc ctgctggccg agctctatga cacgctcgac
    10321 gcagaagcgc agcagagcat ccgcgacctc atcgcacttc atgccgaaaa aaccggaagc
    10381 acccgcgcca aacggttgct ttctgactgg gagcgcttca gcggacgctt catccgcctg
    10441 acgccaagac cccaggcgta ggttcggggg gtcggtttcg gtgcctcatt ctgaatgggg
    10501 cacccctttg aaatttccac ggccgggaag gctcacaacc gcttcaggta ttccttcgcc
    10561 tcttccagat gcggaaagag cttttcctct tcctgaaact ccagtgaatg cacgcaccgt
    10621 cttcctccgc gcacttttac cgggtgtttc aggtgcagca tctcgtgata caccagatat
    10681 tcaatggcat aacgcggcgt gttcttccgg tcaaagacgc ggctgatcat gatggtattg
    10741 tgcgccgcat cataatgccc cagcagccgt tttgctttat gctcgctcca tgtaagcacg
    10801 ggacggccca tcagcccatg aaagaaccgc tgattgagtg actcaaaaac ctcttcaagg
    10861 tcataatgtt cacctttggc cgtggatatc ttcttgcgcc cgcgcatctg gcggacgcgc
    10921 tccgtctgcc gcatcacatg ctcgctcgaa gcaaaacgcc ggtagcgtgt gacatgcgcc
    10981 ggatcaaggg acttgcggta gagctttgcc agcagaatgt gtgcgatggc ccgcagcacc
    11041 ggctgaggag cgccctccag caaatcggaa agccgtacct tcaactcgcc atcgcgcagc
    11101 cggatggtcg tattcaggcc ggcaaagtgg taaaagcgaa catgaatcgg aggaatggga
    11161 gcacgcggac gaagaacacg gtactcctcc tggaacaatt gcagaacttc agggtgcact
    11221 gaaccagagc gtaacataaa ttcccgcggc gcaggtttcc gtgctaccgt tgaacgaatg
    11281 cgccgcgtca tctatgaggt cgcatgttcg ctggacgggt acattgcccg cctggaccat
    11341 tcctgcgatt ggctcccaaa tgacgaacag gattacggcc tgaaggactt ttatgcacgg
    11401 ctggataccg cactcatcgg ccgcaaaaca catggcctga tggtgaagca tgggcagcct
    11461 gccatgccgg gattgactaa ctatgttttc tcccggaatc cgccatcggc gacatacgaa
    11521 ggcgtgcagt gggtcagcag cagtcctctg gaacttattg gaacgctgaa atcgaagccc
    11581 ggcaaggaca tctggctggt aggcggatca aacctcgcac agacgctctt cgggtcccgt
    11641 ttggtagacg aagtttcgct ggcgatcgtg cctgttttgc tgggcgacgg aattccgctg
    11701 tttccccggt ttaccgccga aacgccgctc aaactcatag aggaaaaggc atatccgggc
    11761 ggagtggccc ggctaaggta cgagcgcgtt tcctgaatta ccgtgtaaac caaccggagc
    11821 cgcgcagaag ggccagctag aatggcgttg aagagagtgg ctgctgctgt gtcatacagt
    11881 gcaacgcacc cagtccccag atgaagtcgc cgcagtggat tcctgcgatc tccctgttcg
    11941 ggaagagttc tgcgagtata tttagcgccc tgcggtcatt gggatcatta aatgtaggca
    12001 caagcaccag tttgttggcg atgtagaagt tgccatagct ggccggaagc cgctggccat
    12061 cgaagatgac gggccccggc attggtaact cgataatgcg aaatggctgc ccatcggggt
    12121 tgcgtgctga tcttaaccgg tcgagatttt cagccagcgg cagatggttt tcgtcatcgc
    12181 ggtttggctc tgtgacagta acgatggtgt tttcattcac aaaccgtgtg atgtcgtcca
    12241 catggccgtg cgtatcgtct ccggcgatgc cgcgattgag ccagatgacc tggtcaattc
    12301 caagatagtc gtgaaagacc tgctccagtt gctcgcgact gatgcccgga ttgcgctgtt
    12361 gtacttcact aagcaggcac tcttccgtgg tgatgagtgt gcctgcgccg tttacatcaa
    12421 ttgagccgcc ttccagcacg acgcgatggc cgccaatcat cggtgtccat tgcggcaacc
    12481 ggagttcttc ggccacatgg tccgggacct gattgtcgag ctgccagttg tcgtacttgg
    12541 cccaggcatt gaatttccaa tttgtgatgg caagctggcc ttgcgggttc ttaataaaaa
    12601 tcggccccga atcgcgcgtc cagacgcgat tggtcggcca ctggtggaaa tgcacccggg
    12661 caagattggc tgctgagcgc ttcagaatgc ctttggcgcg cttctcggcc gccgcatcgt
    12721 tgaccagaat gtggacgtct tccacgcgcg agagatggcg aacgatgtcg gtgtagaccc
    12781 atgggatggc ctgaaatttt cccggccagt cattggcatt gtgcggccat gcaatccatg
    12841 tggctgcgtg ttcggccaat tcggcgggca tccggtatcc gtgttcgcgt ggcgttcctg
    12901 tttcggtcat ctctatgtat gcgatgtttc cagatatttc cttctgagga gcgtagcgaa
    12961 gaaggacctg tagctcagct cgcttgtcag atcgttattt ttcgggaccc gtggtgtcca
    13021 gaaagcggtg gacaatcggc gcataagcgt caatacggcg gtcgcgcagg aagggccaat
    13081 tgcggcgtac atcttccagt gtgcggacgt ccacttcgcc aatcaggatt tcttccttgt
    13141 cgtgcgaagc tttggcgata atgcgtccga agggatcagc aatgaacgac ccgccccaga
    13201 attcaagccc tggaccttct acgcggtttc cgcgcacatc gccctgttca tgcccgacgc
    13261 ggttgactgc tccgacgtat acaccgtttg caatcgcatg tgcgcgctgg atggtttgcc
    13321 acgcctcata ttgcgctgtg ccgtactgtt ctttttcggc aggatgccag ccgatggctg
    13381 tgggataaaa gagcacgttt gcgccctgaa gcgccgtaat gcgcgcgcct tccggatacc
    13441 actggtccca gcagacgagc gtgccgattt tgcctacggc ggtatcgaat gccttgaatc
    13501 cgagatcgcc cggggtgaag taaaacttct cgtaatagag cgggtcgtcg ggaatgtgca
    13561 tcttgcggta gagcccgccg atgctgccgt ctttctgcag aatgacagca gtattgtggt
    13621 acaggcctgg agcgcgccgc tcaaagaggg aagcgacaac gacggccttt tcttcacggg
    13681 ccacagcgct caggcgttcg gtgctgggtc cagggaccgg ctctgcgata tcaaaaagcg
    13741 catggtcttc gcgttggcag aaatactgtg cgcgaaaaag ctcaggcagg cagatgacat
    13801 ttgcgccctg gcgtgcagcc tcgcgcactc gctcaatggc tttttccagg ttcgtgtccg
    13861 gatcaggtgt gcaggacatc tgcaccaggc cgattttgta gttcagtgaa gatggcattc
    13921 gagttctctt ttaagacgta taccgcagct ttgtttcaga acagagaaaa ggcgcaggca
    13981 ccttcacctg cgccttcgtt ctgttcctat tctattcaaa gatgagaatt gccgcagcta
    14041 tcgttgtcgt ccaacggcca tgcttgtcgc cgatagcaga ttgtgtcaca ttcgccgtgc
    14101 gcacaatttt atttgagatg cggtagattt ctttcttttc gtcccagctt ttgtccggat
    14161 cgaattcaag attcagcgtg gtggcaagca tctcggcggc caattcttca gcgtattctc
    14221 cggcctgctc ttcggtctcg ccaaagctgt ggtgttcact cagatagcca taggtgttgc
    14281 ggtcagaagg gatagcaaca ccgatgctgg aggcgatgag ccggtgcggt tcgcgcgtgg
    14341 agttctcggc aacaacggcg aagacgactt cgccggggtg caggtacttc agtccctctt
    14401 ttctgggaat cagttttgcc tgcgggggaa aaatggatga gacgcggacg agattctgcg
    14461 cagcgattcc ggcgtcgcgc aaggccaatt caaaggatgt aagccgttcc ttatgctttc
    14521 ccacgccttt ggtgaaaaag agccgcctgg gaaccatgct tttgcccaat tccgcgccct
    14581 cctgcgagga agttcacctg ccggatgaaa agtaacacac ttttcataga gttgggatgc
    14641 aaaatttgtg agatcaggcg gtttgttgtc ataccgtttg cgcttcttgt gttgtcggcc
    14701 ttgcgggtat cgtggtactg ttgcctgcgc acggtcaaat tgtcaggtgc agacgttcag
    14761 acgttgaaat cgcagtattt aacccaagga gggcaaatga aacgagtact cgcggtaatg
    14821 gcaagcatct gtctcacctt gccgctcatg gcacaggaga aagaacggca gcgcatcaaa
    14881 gattcagcac aggtgttgag tgaaattctg gccacgccag acaagggaat tccgcaggac
    14941 ctgctggaca aggctgattg cgtcatcata tttccttctg tcaagaaagg agcattcatt
    15001 gtaggtggca gctatggacg cggcgtagtc acctgccgtt ccggcgtgga tttctccggc
    15061 ccatggagtg ctcctgcaat gtttgccctg gaaggcgcaa gtgtcggttt ccagattggc
    15121 ggccaggcga ctgatttcgt cattctggtg atgaatcagg gaggggcaaa ctccatcatg
    15181 tccagcaaag tgaaactggg tgctgacgcc tctgtggccg caggtccagt aggccgcaac
    15241 accagcgcgg agaccgacat tgtgctgaaa gcgcagatgc tttcctggtc gcgcgcacgc
    15301 ggcgtgttcg caggaatttc gctggccggg tctactctgc gttcggacga tggggcgaac
    15361 aagaacctct atggaaagga cctgacagca aaggaaattg ttcgcgaagg caaggtgaaa
    15421 ccgcccgtat cggcacgacg cttgttagca attcttacca aggcatcacc acatcacaag
    15481 gcatagccga agaaaatctt accagggcct catcaagcga ggcccttttt ctatccactg
    15541 atggagggaa gccgctctga agcacccagt agaaagatga atccgatggc caggctatag
    15601 accgccccgg ctgctgcaat catgcggtaa aagttgggat gcgcccggcc tgtaccaaaa
    15661 atgccgctca gcgcggccgt cataattgca ttcatgatga ccaaccccag gccgaaagcc
    15721 agcagtccaa gcaggccgag catggtgcct cccaagcttg ctgtcagcag aaagagcata
    15781 agttgcgttg gtgtttctgc gcccaagcca tgcaatatgc caatacagaa aactgcgccg
    15841 ttggagtagt tccaggtgaa gggcttgggt tgaggcgcgt ctttttcaat gagacggcgc
    15901 actcgccagg cagcatggcg cactgcgttg atgagtagcg cccagcggct ttccatggcc
    15961 tgatgcgaat gtgaatgttg ccgcttacgc acaaggtttg cgacgacaag aagccccagg
    16021 acgataagcg tgaatccgac tatccgttcg gcccaggcat cgagccttgc cggaagcgac
    16081 agatgcaaaa gaattacaag cgcacccagc gtcaaaacgg tgaaagcatg ccccaaagca
    16141 tagagaatgc caagacgaat gccttcaaag cgcgaacgct gcactgctgt aatgtctgaa
    16201 atggcggcga ggtggtcgta atcaaaccca tggcgcagtc ccagcagcgc gcaggagcca
    16261 atcgcaagtg caagcttcca gtgcatgacg caaacagtaa caagcagtct accgtatctc
    16321 tggaggagca gggaaacggg cataggaagg cgccagtaaa aaagtagaat tgacgtagtg
    16381 cttgtttctg attttgacta cgatctccct gaagaccgca tcgcgcaaag gcccctcgcg
    16441 gaccgggccg gatcgcgcat gctcacgctt gctcgcgcca caggcgactt ccgggaccgc
    16501 atgttccgcg actttccttc gcttttgcgc gaaggagacg tactggtact gaacaatagc
    16561 cgcgtgattc ctgctcgcct tttcgggcac cgggccggac tgcacacgca atcgcaacac
    16621 acaaccacag ggcgcgttca ggtgctgctt accgagcaga tcagcgaatg ggagtggcgc
    16681 gtgctggcgc gtcctgggcg taaggtgctt aaaggcgaag tgctgacgtt tggggacaac
    16741 gatctccgcg cggaagttgt cgagcacggc gattttggcg agcgcgtact gaagtttgaa
    16801 cctgttgcgg atttttacgg catcctggaa aagctggggc acatgccgct tcctccttat
    16861 atccatcgtg aagatgaaga cgcagaccgc gagcgttacc agaccgtgta tgcgcaacaa
    16921 cggggctcgg tcgctgctcc cacagcaggg ctgcacttta ctccggaaat tctggaggaa
    16981 atacgcaagc gtggagtaga gattgtctac atcacgctac atgtggggct ggggactttc
    17041 cagcccgtgc gcgtggaaaa agttgaggac attcatctgc ataccgagcg ttacacactg
    17101 tccgatgaaa cagcatcggc gatcaatagg gccaggaatg aaggacggcg tatcgtcgct
    17161 gcggggacga caactgtccg aacgcttgaa cactgtgccc tgaaagcagg tgaaaaacca
    17221 ctgggaccac attccggttc gacagacatt tttatttcgc ccggatttga atttcgtatc
    17281 gtaaatgcac tgcttacaaa cttccatctg ccgaagtcca cactgctgat gctggtaagc
    17341 gcctttggcg gaaaagagca tgtgctgagg gcctacaacc atgccgtaga agcgggctat
    17401 cgcttcttct cctatggtga ctgtatgttt ttatcttaag ggcgaagaag agacgaaaat
    17461 agcggtaaaa taggccgatg cagtctgtat ctgttcctga aagtcttgcg tgggcgcacc
    17521 ctgtggtgca ggagtggttt gtgcaccgct tcggcactcc tactgagccg caggagcagg
    17581 gctggccctg tattctggcc gggaagccaa cgttgatttc agcgccaaca ggttccggca
    17641 agacgctggc cgcattcctc atctgcattg atcaactcat ccgcaaggca ctggaaggac
    17701 ggctggggcc ggaaacagaa gttgtctatg tttctccgct gaaggcactg tcgaacgatg
    17761 tccagaagaa cctggaaacg ccgctcaggg aaatccagca gcttgcgctg gagtgcgggc
    17821 atcttgcgcc acagatacgc actgctgtgc gcacgggaga tacgctccct gctgagcggc
    17881 agaagatgct gaagcagcca ccgcatattc ttgtaaccac gccggagtcg ctctacatca
    17941 tgctgacggc ggagaagagc cgcaacaatc tgcgccatgt gcgcaccgtg attgtggatg
    18001 aaattcacgc gatggtggac gacaagcggg gtgcgcatct tgcgctcacg ctggagcggc
    18061 tggacgcttt ggtgcgcggc gagaaccgtt tgtctgtggg tgaatggctg acaggacaca
    18121 acgaagcgcc gcagcgcatt gggctttctg ctacgcagaa cccgattgag ctggtcgctg
    18181 aatttttaca aggtggatgc aggcagcgag tcgaaattgt gcaggtgggg cagcgccgcg
    18241 agctggatct ggcgattgaa gtcccagatg aagaactggg tgcggtggcg aacaacagca
    18301 tctgggacgg catctacaag cggcttgccg aacttgcatt ggaacatcgc tcgaccctgg
    18361 tatttgtgaa tacgcgccgc atggtggagc gcatcgcctt ccgtatggga gagctgctcg
    18421 gcgaagaaaa cgtcgctgca caccacggaa gcctttcccg caagctgcgc ctcgaagctg
    18481 aacgcaagct gaagcaaggt gagattcgtt tgctggttgc gaccgcatcg cttgagctgg
    18541 gtattgatat tggcaatgta gaccttgttt gccagatcaa ctcaccgcgc gcgattgctg
    18601 ttgccatgca gcgtgtgggt cgtgccgggc actggagagg ggccatccca aaggggcggt
    18661 ttttcccaac gacacgcgat gatctgattg aacaggcggc aacgattcgc gcgatgcgtg
    18721 ccggcgtgct cgacaagctg gaggttccgc caaagccgct cgatgtattg atgcaacaga
    18781 ttgtcgcggc ctgcgctgcc gaaccttggg aagaagatgc gctgtttcaa gtgctgcggc
    18841 gcgcccaacc gtatcactcg ctcacgcgcg acgagtttga agaagtgctg tgcctgctca
    18901 cggacggcat cgaggccagc cgcggccgct acggctctta tctgctgcgc gaccgcgtgc
    18961 atgggcgcat tcaggcacgg cgcggctcgc ggacgattgc ggtatccaac gcaggtacga
    19021 tcccagatac ggcgctctac gctgtcgttg cagaacccga aggcgtgcag attgccacac
    19081 tcgatgaaga ctttgccgta gagtcgtcgg cgggcgacat catcctgctc ggcaatacaa
    19141 gctggcgtgt ggagcgcgta gaaagcgcag gccgtgtatt tgtttccgat gcgcagggcc
    19201 aacctccaac tgtgcctttc tggcgtggag aggcgccgca gcgcaccatt gagctttcgc
    19261 agtttgtctc tgatttgcgc gaagagatag accgtcgcac aaaaaatgtt ctccctggat
    19321 acatcggcca atccaatcct gaaattgcgg aagctgttac atggctcaag caggagtgtg
    19381 gtgtctgcga ctccggcgcc gaacagatca tcggctacat cgcagctgga cgctctgtgc
    19441 ttggcactgt gccttcgctt acaaacattg tcgccgagcg gttctttgac gaaggcggcg
    19501 gaatgcagtt ggtcattcac gcgccctttg gtggacgcat caacaaggcc tgggggctgg
    19561 cgctgcgcaa gcgcttttgc cgcggtttta attttgaatt gcaggccgca gccacagaca
    19621 atggcctgaa tatctcgctt gcagaacagc acagttttcc actgagcgat gtttttcatt
    19681 ttcttacaga acagacggta cctgagcttc tggaacaagc atcgctcgcg tcaccgattt
    19741 tcaaagcacg ctggaagtgg gccgccggac gcagcctgca actgctccgc tacaagaacg
    19801 gcaagcgcat acctccacag attcagcgga cacggggcga tgatctactc gcaagcgtct
    19861 ttccgcaggt ggccgcgtgc tttgaaaaca ttgaaggcga tattgttatc cctgaccatc
    19921 cgcttattcg cgaagtcatg aaagatgtgc ttggcgaggc catggacctt gaagggctac
    19981 agcgtgttct tgccgacatg cactcgggaa agatccgctg cgtcgcagtg gatacgccca
    20041 ccccttcgca cttcgcacac gagatgctga atgcgaatcc gtatgcgtat ctggacgatg
    20101 caccgctgga ggagcgtcgc gcccgcgctg taaacatgcg cggcattctg ccggacaatt
    20161 tactggggga ggcgggcagg ctttctcctg cggcaattgc ctccgtgcgc gaggaggtct
    20221 ggcctgatgt gcgcgatgag catgaatttc acgatctgct ttgctcgctg gtgattgtgc
    20281 ctgcggagat attgctggaa ccgcaggccc gaaattggga cattttcttt gcccggctgg
    20341 aagagcaagc aagagtgtgc atcgcacagg ccggtggacg tgaatatctt tgtgccgttg
    20401 agctgatgga gtatctcaag caggtgttcc cggatgtgca gtttgcgtct agttcagcat
    20461 atgtatctga tccagctaca catgaagatg cgctgcgtaa agctgttcag ggatggatgt
    20521 ccgttctcgg cccggcttct tcgcgcactc tcagcgagaa actgggtatc aacgcaggcg
    20581 aagtttggaa gcagatgctg caccttgaga tgacgggcac ggttcttcgc ggagtctttg
    20641 aagggcaggg ccgtcctgaa ggggccctgg cagatgaaga tgtagagtgg tgcgaacgcc
    20701 gcctgttgca gcgcattcac aaacgcaccc tggttgcgtt acgtaagcag gttgagccag
    20761 tatctccgtc aaccttcatg caatggctgc tgcgctggca gcacgttgcc ccaagaacac
    20821 agctcagcgg agaggccgga cttctcgaag cactccgcaa attggaaggc tttgaagcgc
    20881 ctgccatcga atgggaacgc tccattttgc cgctgcgcgt ggctgggtat gatccgcgct
    20941 ggctggatgc gctctgtctt tccggtgttg ttggatgggg gcgcatctct ccgcatcctg
    21001 catttaactc catcgaatca ggtgggccgc ggcgtgtggt gcctaccagt atggctccca
    21061 ttacattttt tattagagaa gaggcattgt ggatggattt gtgcctgtca cagcgacaca
    21121 ttccggagcc ctgcctgaat gcatgtctga gtgaagttgc cagtaaagtg cgaaatgcgc
    21181 tttcgtcgca gggagcaatg tttgcaggtg atctggtgcg caggcttagt gttccatctg
    21241 cggaagtaga tatcgcgtta tgggaactgg ttgcagcagg gctgataaca gccgatggat
    21301 ttgacagcct gcggatgctg attgatcccc ggcgcaatct gggccgcaaa accaaacagg
    21361 ccgcaggccg gtggagtttg ttcagaagta cggatgaaca gacgcccacg cggcgggaaa
    21421 gagaaatcga gtctgcctgc cgtatgctgc tgcggcgtta cggcgtgtta ttccgcgatt
    21481 tgctggagcg cgagacaacc atcccgcgct ggcgcgattt gctcggaatt ctgcggcgaa
    21541 tggaggcccg cggtgaggtg cgtggcgggc ggttcgtctc tggttttaat ggcgagcaat
    21601 ttgcgctgcc tgaggcgctc gattcactac gagaggcgcg tcgaagcggg actcagtttg
    21661 cgccagtgag cgtggctgcg gctgatccat tgaatctgat tggcattgcc gttcccggcg
    21721 agcgcgttgc ggctgtggcc ggaaacaagg tgatgctgga cgaggcgatg ttgtttccca
    21781 ccctttcaca gaagccagga aaggacagcg cacctaagtc taatggcgtg gctgtttctc
    21841 cgttgcttct gacagaatcg aagtatgagc agccccccac agcctgatca accacagccg
    21901 cttatcgaag gggtggatta ctacatggac ggaccgtatc ttgtttttac tgaggcatat
    21961 catctcaagc gcgggcattg ctgcgggtca ggatgcaggc actgtccgtg gaagaatacg
    22021 gagtcagaaa aaagcagcaa cgcctgagcc gtacgaatgc aaacatctac ggcgcatctg
    22081 gaatcctcag tcattgctga ggagcatagc gaggaggcct taacgtccgc ctggcgatcc
    22141 ggaaccggat gatgacgttc ctgctggttg tgctgtattt gcgccttcac ggatgaggag
    22201 tgctgttcga gggaaggtga aactgccgcc cgcgtcatca attacattga tctggcaggt
    22261 ataaagccct ggctttaaat cagtcaacgg aacatcaaat tgaaacgcca ccgcatcccg
    22321 tgatggttcg ttgatttgcg tggcctgcac catgggcgtc tcaaacacct taagtccatt
    22381 gctcaggaac tcaatgctgg tcatcactcg cacaggagcg gggggcgcat ctttagcatt
    22441 ctttgttttg gctaaagaag ttgttatttc ctgaccctgc cctgctgctt ttgccggatt
    22501 gtatacctca tagagaaaat acagatgctg gttctggcgg aagacatgcg ccaggtttgg
    22561 caccagttcc tgtccgtcgt ggaccagggg actgtccgat tttttgttgg cgggaatgcg
    22621 ctggctggcg agcacaacgg agctaagtct gaccggagct ttacgcagat ccggaacgtt
    22681 gatatctgtt tcaaacgatc ccatgcgtcc ggtctcgttt tcgcgaacaa caaacttcag
    22741 atgatatttg ccggcaggca gcgtgaagct agtggagtat tgaatgtttt tctggcgcac
    22801 ctgctgtgat tggtccacgg cgagcttgac agtatcacgc gcatcgccaa ttcggcgtcc
    22861 agcggcatct tttacttcgc cgataatatc gagcgtggct ttgtcgcggt ccccgccttt
    22921 aacgaagggg atctgtgatc cgggaacaag aatggaaata gggatgtaat agcggttctc
    22981 gtccattcgg aaatacagtg cctgcaaata gacggcaaca tcggttgcag gcaggtcact
    23041 ggcaagctgt tcttcaagtt cgcgttcgcg gtcttctttg tttgaatgcc tgaagtcggc
    23101 gggcgcatag tagccatggc gatactcaag ttttacgtcc gggcggtcca ctttgacagt
    23161 gagccggcga tacgcgccat cacgacgcgg gtcagtagaa cggtagccga tgacgtagta
    23221 ggcggaagtg tcctgctgca tctgctggaa ggcaggtgcg aagtcgttgg agtcaaagaa
    23281 ggccttgcca ccggtgtcag atgaaagatt cgccatcact tcctgcgaat tgaaattggc
    23341 gtcaagattg ttctgaagtg ccgcgccatt gtatgcactg gttccgcgca ggctgcctgt
    23401 gctggcatca cccagtggcg agatggcttg aagcccccgc gcatccacgc tgtagatgga
    23461 catattggaa cgtaccgacg cattaattgc agagcgcaga gaagcctggt tttcgattcc
    23521 gtcgcgcgag atgcctccag agaaatagac cagcgacttt ttctggttga tgtagttcag
    23581 cgacttagca atcgaagcaa tggcgtagag ttcacggtca gtgttgatgt cgttgtattc
    23641 gctttcatca ggagtataag cgctggtgtc ttctgtctgg ttcgtagtgc tcgtagcgcc
    23701 cggcgcgtag ccctgtgtct gatcgcccga atagctgtta atggcgttca gtaaaagctg
    23761 cttattctgt gtgaagtcct ggtcaacaga gatggacgta tcgagcgaga caacagcgac
    23821 cacatcggcg ggctgcatct gtttattgat gtagttgcgt gccgcatcct gagcgcgctc
    23881 cacatcctcg ggttgcatcg aggtcaggtc gaaaaacaga attacaagcc gatgatcacg
    23941 gagcgcatct ccagtaatga ctcccgtttt gccaagtaca atgtttccgg ccctgccgct
    24001 aatcgttgcc tcgttcaggg ctgcggcctc gtccacactt tcaaaatcaa aactaattat
    24061 ctgctgcggc tttccgttct ccgtgatggt gaaatcattt tttgtaagcc cctttacgat
    24121 ctgccctgtc tttttatcgc ggacgacaac attggtaagg acaagatcac tgttgacctt
    24181 cagggtgaag gattgggact gctggttgtc ctgctgggcg taaagaggga gaagtatagt
    24241 gccagcaaga agcaaggccg tcgcgcatgt cctgcgccgt aacaggagtt ccgcagtatg
    24301 gtgtatggat tcaagaagca ggtccttcgc ttcgcccagg atgacaaatc ttttgctctg
    24361 cgtcattgct atgtcctcct tagaagcgat atctgcccat gaaggtgaac tggcgcatag
    24421 ttgccgcgga ggtcacctgt ccataggtag ccgagccaag cgtggtatct acgccggaat
    24481 actggacggt attgaagacg ttgttcgctg tagcgcgcag ttcgaggttt cggaaatctc
    24541 cgaaacgtat gcttttggaa agtgaggcat caaacgatac ggtccctggt cctggaatcg
    24601 aataacgtga tgcggtaccg tacgttccgg caggctgcgt gaaggcggca cggttgaacc
    24661 agttgcgcag cgaacctccg ccctgtgtca gggaaacacc ctggacgcga tccgggcgca
    24721 gggagccggc gctaccacgg gccacgtctt ctacagcagc tgcataagca ggagtcagtg
    24781 gttcgccggt cgcgaactgg aacgtgccgg acaaggagac gtttgcaagt gtgtgagaaa
    24841 gccagttgcc attggtcagc atgtgcgtat caggaccaaa gggaagctcg taaacgaaat
    24901 ctccttttag attatggcga acatcgaagc tggaattgga ttcctctgcg agcagatttt
    24961 gccagttctg tgcaacaacg aggccagtgc caccattgcc gccaatcgag ctggcattat
    25021 caattgaatg cgagtaagtg taggtcgcac cgacggcaat tccattctgc atacgcttgc
    25081 gcagccgaac agcgagtgcg ttgtagttgg aaaatgctat cgaatcttcc caatcgtagt
    25141 agtagccgct gagcgtctcg gtagctgtgc gaccgggtgc gctgacaata tcgagacgcg
    25201 tgcctttcga cccgttgtag ccaacattca tcacaatgcc aagcgggaag gtgcgctgga
    25261 cgtctatgtt ccacacttga acgtaaggaa ggcggtagtt cttgttcacg gcatagtttc
    25321 caaccgtctg tgggccggga aaaccattgg ccaatgtaat gctggcaccg gacgtgactt
    25381 cgtttgtctg tacatcggca aatggtggct gaaaggcaaa gttctgcaca aatttcacat
    25441 attgcccgtt ggcgaaattg atgccgtagc ctgcgcgcac cactgtgttt tttacagcgc
    25501 gccaggcaaa gcccagacgc ggggaaaaat tgagacgctc aggctcaatc agcgtgcgcg
    25561 gatacttgcc ggagtaaggc cctgttccgt tgggatacac gggcacaaca gaagagaagt
    25621 tattacccac gtcaaggttc acgatacggt catttttctc cgagtacgga gaaaagtatt
    25681 cgtaccgtag tccggccagc acggtgaaat tcggcaacgc ctgccaactg tcctgaaaat
    25741 acgcatcaaa gacgttctca cgcagatacg ccttctcgta gggagcctga agcgcggtct
    25801 gttgcggaag tccaagcaga aggtcggcca gcgaggagcc gctggtggca agcgctccgc
    25861 tagtgtctgg aattgtgcct ggcgcctcgg taaagagccc ggtgaagtag aaggtgcctg
    25921 tggaattgct ggtcgcgcca atcatgtcca ggtgcacgcg acggaagtct gctccgaagc
    25981 ggaagttgtt cttcttgtgg atccacgaac tggattccga aaccgagatg gtctggttga
    26041 tgtgaaagtt cggctgctgc tcgttgaatc cggtgaactg gttgaggacc aaattaggga
    26101 gcccgtaatt cagcggagac acggccgctc cattgaagat attgatgcca agttgccggg
    26161 cgatatcggt cgtgttggtg aagtagttcg tcagttgggt gttgctgcgg ttccagccaa
    26221 cgttcagatt attcaccagc ctgcccttgc tcagcgagta gcctgcctgt accgagtact
    26281 gatgcatctg ctctttgccg cccaggtcgg ggaagatgtt cagctcgtcc gcggccgtat
    26341 ggctgtaatt gaagttaccg ttgatgcttt gatgccagct attgcctcct tgtcccataa
    26401 attgccgtat catgcccatg atgggtgatc ctgcactgga accaaaactg cgcatatagc
    26461 gcaggccaat gtttgtggta ttgttttggt ttgttgtaat gcgttgatag ttctgtgccc
    26521 gcccggaaag attgggcaac gggatgtaat tcagtaaggc cgttgcctgt ggagcaatgc
    26581 gctgggctgg gatcgtgtta ttgggaaaag gttggccggt ctgaggatca tagatggtaa
    26641 tcggctggcc ctcctggttc gtaagcgtag agagatcgcc agtgcgctct gcggctgtcg
    26701 gcacggtgcc atattgcgta aagggtgaag atgtacggtt acctgcaagc gtgaagaaga
    26761 ggaagtcctt agtatcatgc tcaagcagct ttgggatgta aggcgcgccg acaaaggtca
    26821 gaccgaagtg gtttgaatta tatgcaggct ggttcagtgg cgcgcctcga acagaaaatg
    26881 gttcggcatt cagggccgaa ttgccgccat tccagaagaa ggccccatgc ggctggcttg
    26941 gattaaattt gcggaagttg ccgcgtccgc gtgggcctcc tataaagact gcacctccgc
    27001 cacctccagg cggaggtggg ggtggaccgc cacctgggcc accaatgtaa atgccttcac
    27061 ctccgccacc gcctttgctt tgtgatcctg gggtctgcga gagcgagcgc tcgaactgca
    27121 tgttctccat gttttcgcgc atttggccga tgtccatccc agcaaatgga ttggtggacc
    27181 cgctttgccc gctgacggca acggactcat tggagaaatc agagttgttg gccaatgcag
    27241 gcaaggacgt atcggaagca gcattgccgc ttccggcttg aatcaaacct gcagcggcgt
    27301 tcaccaaccc caggttttgt aaaccacgaa gagtctgcaa agcctgcgca gcctgggttg
    27361 tgccttcctg ctgctgtgcg cgcgaggcca gcttgagtgc aaagtccgct tgctgattct
    27421 gcgaagatgc tgcattgaac agcacttcct ttgtttccgt agcaaaagca gcaaaatccg
    27481 tccgtactac gtagcggccg ttctgaggga tattcagcga gtaggccccg ttcacgtctg
    27541 tgactgtgct gtatttcttt cctgtcagcg tgtttgtcgc ggtaatgctc acaccgggca
    27601 acggtacgtt tccggatttc accgaaccgt ggactgttcc gccagtagtg gaaggaccct
    27661 gcgtttgagc gacgcagtaa gttgagaaga ccagcaaaag gcagattggc aacaagcggc
    27721 gcagcacgta tcgtctcccc cgatggaatg gattattgcg gctgttgtgg cgacggaggg
    27781 ccgtttccag acgcgggagc gctgccttgt ccgggtcttc gcggctccat aacattcaat
    27841 acggaagcaa cgaagtttcc gttctgcaag gccccgcgcg cagtcaccat gtcgccgacc
    27901 ttgatgtcag cgagcgtgat gctctcgcgg tgcttctgaa aagaggtgtt ttcgttcacc
    27961 gtgatggtct gggtcttctt atcgggccgc tgaacagtaa gcgtcagatc atttatggca
    28021 gtgattttcc ctgcaagcca ggtctttccg aaatccgcct gcatctgttt catgcgctgc
    28081 accgcttcag gaccgaggac cccgaggaaa gccgcgccga ctgtgtgggc ctgctcgtcc
    28141 aattctccac ctgccataat cacgtcaccc acatgaacat cggacgccgc cgccggttgg
    28201 cggtctttca taaagcgagt atttgggccg gtttctacct tccaggtctc tccctgctca
    28261 ttcttcaccg taaattcgtt gccggagatg gctgtgaccg tcccaagaac acctctcccg
    28321 aaaggagcgc cccattggcc gccttgctgc caggcatagg ctgacggaag gagaccaaga
    28381 aggacaagaa aagcaagagt gcggattttc attttggcct gcctgaggat tggctgccag
    28441 tttagacggt tgttttgtcc gtggagtctc aaataaggag ggattttcgt tctgcttatc
    28501 gagaagaaag tatgtccctg cgattaaaaa gaaacctggc tgtctgtcac agaatctttg
    28561 caagaagcgg gcaacacgtg gcgggaaaat ctctctaagt taaaggaagg aggccggatg
    28621 agtcaggcag aagcggtttg ccaatcattg gcgcttgcat tttgtagtgt gcggtcgcgg
    28681 agcgagttgc tttgcgagcc gcttacgccc gaggacatga tggtgcaatc ctgtcccgag
    28741 gcaagccctg ccaaatggca tctggcgcac accacgtggt tttttgagac ttttatcctc
    28801 agcgagttct gtcgcgatta ccgtcccttc catcctgatt ttctctggct cttcaacagc
    28861 tattacaacg ctgtgtccga ccagccggaa aagaagctgc gggccgtctt ttcgcggcca
    28921 tcattaacag agattctgga ctaccggcat tctgtcaaca gggcgatgga aacgctgctg
    28981 cagggacaac cttcacctga aatcgagcag cgcgtcgtgc tgggaatcaa tcacgagcag
    29041 caacatcagg aacttttgct gactgatatc aagcatgctt tctggacgaa cccgctgcat
    29101 ccggcgtatc tcgatcttcg gtttgagaag tctgcggccg gagatgcgga tgcgcgaccg
    29161 caatggtgcg aatatcctgg cggactggct gaaattggcc atcaagggga cggcttctgc
    29221 tttgacaacg aactgccgcg gcatcgtgta tttcttgagc cattcgcgtt ggcttcgcgg
    29281 ctggtgacct gcgcagaata tcttgcgttt atggaagatg gcgggtatga gcgaccaaac
    29341 ctttggctgt cagaaggctg gcagactgtg caatcgcagg gatggcgcgc tccgctgtac
    29401 tggcataaag aaggagacga atggagcatc tttactctgc gcggcaatgt ccgattgtcc
    29461 gatgtcgcga acacgcccgt atgtcacgtc agctactttg aggctgatgc ttatgcacga
    29521 tgggcaggga agcggctgcc cactgaagcc gaatgggaag tggccgcatc cccacaggat
    29581 gtgcagggaa atatgcttga gtccggggcg ttgcatccag ccgtcgcaaa tgcgggccat
    29641 aggggtatgg agcagacctt tggagatgtt tgggaatgga ccgccagcgc atatctggga
    29701 tatcccggct ttcacgcctt gcccggagca ctgggcgagt ataacggaaa attcatgtcg
    29761 ggccagatgg tgctgcgcgg cggttcgtgc gtaacgccac agacgcatat tcgcgccagc
    29821 taccggaatt tctttgctcc ggccacacgc tggcagtttt cagggattcg tcttgccaat
    29881 taaaaacata aggacaaaca tacaatgaat gcgctttccg cagtacccca tctttccccg
    29941 gatatttttg ggcctgtcgg cgccgaagtt tacgcggggc tgacgagttc gcccaagcgg
    30001 ctcagtccgt ggctctttta tgacgggcgc gggtcgcagc tctttgagca gattacagaa
    30061 ctgcccgagt actatctgac gcgcaccgag cgctccattc tgcaggccca tgccgatgaa
    30121 atggttactg ctgccgccaa ctgcgaccgt atttcagtga ttgagctggg agcagggacc
    30181 gcgaccaaaa ccgggctgct gctggaagcc gcggtccgca ggcaaaagac agttgcgtat
    30241 tacccgattg atgtgagcga aacgccgctg gcggaggcga agcgacgtct ggaggctgaa
    30301 atcaagggcg ttactgtcgt gccgcgcgtt gccgattaca ctgcgggcct ggggcaaatt
    30361 gcaactgccg gcgagcgacg cctggtgctt tacatcgggt cgagcatcgg aaattttgaa
    30421 ccttctgacg ggttgcaact gctccgcaaa atacgggaaa gtcttacggc tggagacaaa
    30481 ctcctgcttg gcgtggacat ggcaaaagac gcccgtgtgc tgctggccgc ttatgacgat
    30541 gccgcgggcg tcacggctga gttcaataag aacattctgg agcggatcaa ccgcgagctt
    30601 ggcgcgaatt tcgatctcac gcgattccgg caccgcgcgc tgtggaaccc ggagcattcg
    30661 cgcatcgaaa tgcatcttga gagcctggtg gagcagcagg tgactatttc ctcgcttgat
    30721 cttgaaatca gtctggaatg cggtgagacc atccacacgg aaaacagcta caagtttcat
    30781 gatgaagacg tgattgagat gctggagcgc gcaggcttcc atctgtcgga cca
//
LOCUS       DASRBV010000226         7792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_318386, whole genome shotgun sequence.
ACCESSION   DASRBV010000226 DASRBV010000000
VERSION     DASRBV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318386"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..2343
                     /locus_tag="VFA02_08485"
     CDS             418..2343
                     /locus_tag="VFA02_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZU09929.1"
                     /translation="MKLRFFFIALACCLLVVSFHRAEAQASVTENQSIYLHVNGTTGS
                     DSNPGSPTQPLKTIGAAVAKALANNRAGIGTKVMIDPGVYREFVKISPYNQTSAPITF
                     QATTAGTVKIDGANVLTNWYRKNTNIYAYSWKDPVGGCPLPSGWYTGMPYIVQNNEMV
                     FVNGQAMTQVSSVSQMRAGTFFVNSTYQELDVWPPSGTDMNTATVEASARRETLEVDN
                     GKNMVFRGLILQHAASCMNQNGANVYNSSNILFDNVQVYWNNWGGLGISGSNHYTVQN
                     STANYNGGVGLAAYEVKSALYQNNETDYNNWRGEMIGLYDFAQGGTKLMHAHGITVNS
                     HRSYNNAAQGLWFDTDNTNVTVANSQLVGNTVAGLQLEANQGPVTVQNSSMCSNGMGV
                     HLINTNDLTMTGNVFYNNGGQNFQNGNFFLAGKAGGRVFTNFETGQTETTLTENTKLE
                     NNVLTAANSSQFVFNTYLSGSDWSTFIDSLQSNNNYWYNAQQPSAYIVTLGKRVTFGG
                     WKSVSGQDYNSSWSQASTYARGCGVPSPSYTDFHVQAHNAAAYVPAYYMSNGSVSIPL
                     QVKSYGYGSVQLSVYGLPSGVSASFSPSTLTSGNSTLTLKASSSAVAQTVPITVFAAS
                     GSRVHSITLKVGIRPAS"
     gene            complement(2401..3237)
                     /locus_tag="VFA02_08490"
     CDS             complement(2401..3237)
                     /locus_tag="VFA02_08490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZU09930.1"
                     /translation="MNCNLPYWKTQLNGLSAEWEILGHVGNPSPTQKAIFFFLDKDSV
                     RAVAKIPLSVQAKAAILNEAAILRRVKAQYIVPEVLFTDEEHGVVAQSWMQGRHISRR
                     FREEHLDFLLGLGCNGCTIRLSDFRNALATQLSCFDRPKTEMQGIEQALSLLENDEEL
                     MAFIEHRDFAPWNIRLLADGKITLIDWEWAVENSLPWQDICRYFYIQDYLFNESKNVW
                     DILTSNALLQTYARKCRLSKETLRGLTSYYLLRTFFEDWTEGQHKRCAYTLRQIQFLL
                     GS"
     gene            complement(3549..4934)
                     /locus_tag="VFA02_08495"
     CDS             complement(3549..4934)
                     /locus_tag="VFA02_08495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZU09931.1"
                     /translation="MLEVQKDNMANDGGLTTVPSLVARLLIEELSKNSNAYCILTGYD
                     DLPESFDSDIDFMVGAQDFERVPEWIAFIAQQTGTRLFQVIPHEATARAFRLAHLTAD
                     GTTFIQPDSCSDYRHFGKLWLKSDEVLEARRPHPKGFWIPAARHEFLYYLIKRLNKRD
                     FRQEHGIRLSRLYSEDPANCDVLLRRFWTEDTAQRISKMAGTGDWRPLIQKTGLFKEE
                     MLQHPAEGWKGRAISRWRNLTHTFERIIQPTGGWIVFVGPDGCGKSSVIEAVIREFGP
                     AFQQIVRCHMRPGIFPGRIKPGSVVTDPHGKPSRSGLASAAKMLYLAFDYFLGHFMRV
                     LPAMMRTKLVIFDRYFYDILVDPKRVRYGGPRWLLRWIAVMLPKPDLVILLNASPEVL
                     WSRKQEVPFEEVCRQQKMYLELAVKLKHAVVIDAAQPLSDVVHDTAEAIVSCYSKRAR
                     IRLGLGEGAEA"
     gene            5045..6262
                     /locus_tag="VFA02_08500"
     CDS             5045..6262
                     /locus_tag="VFA02_08500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU09932.1"
                     /translation="MKILLSAYACEPHKGSEPAVGWNWMLSLIGQGHEVFVLTRKNNA
                     PAIESEIKKQHLSVTPVYYDLPSWCRRWKHWPGGLYFYYLFWQLGAYFYAKQLHKVQR
                     FDLVHHITFATFRQPSFMGRLGIPFVLGPVGGGETSPRHLRRGFNWSGRLRETLRDIG
                     IAWASHDPLMHSTFSHASWIACTTHETLKQIPGRYHSKCIVQPAIGIDSPQLSSSISQ
                     PSRNNFLFIGRLLYWKGLHLVLRAFPDVLQRIPDARLRIIGEGEDSDWLKSIAQECGI
                     SSAVDWIPWMPHQQIAAEYENNIAFVFPSLHDSGGLVVLESLAASLPVICLDLGGPAI
                     FVDDSCGIVISTSERNEASVQKTLAQAMISLASDPERRNLLASGCQQRVRQFSWHSGA
                     DHLYSFISSEHSR"
     gene            complement(6267..7613)
                     /locus_tag="VFA02_08505"
     CDS             complement(6267..7613)
                     /locus_tag="VFA02_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HZU09933.1"
                     /translation="MSKLKQSVLYESGQRMNLFYMQFLRRRRYQTIADQLVVSGSNFA
                     TGIILVRGMGLEQFGVFTVAYALVLLANSVQLSFIASPMITLAGLCKDEEARMRYLRG
                     MFGVQVKFCAVATALTFFITAIFLWLKPGFARLDQLPSFLLCLVFYLMQDWLRRYYFA
                     VNKSFHSVWNDLISYLGQTIVLLGLMYVHRLTVNTAFLAIAVTSAIAFGAGAAIERLR
                     WSGDELKQSWARSRGISRDLAIANQLQWFVYQGAMLIGASVLGSEAAGSVRATQNVVG
                     PVNVAYQAMENIVPLKASEEMRKGGITRVSRFLLLFSTKGFIALSIVFLGISFFSREF
                     LAFFYGHKVAVYAGILNLQMLYFLLLWPLRQFSYLFRTIGETSAILTSSLAAAITSIV
                     LIYPCIRSFQAMGIMVAAVGGQIANLVFLFLAWNRFRVSNGAKKEQISTEVEISQV"
     gene            complement(7610..>7792)
                     /locus_tag="VFA02_08510"
     CDS             complement(7610..>7792)
                     /locus_tag="VFA02_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09934.1"
                     /translation="IIHVWMTTVMSTGVLGWMGYEMALFLCIMTNLDLVRHRPAYAYQ
                     RVVRVAAPPGLVAVAK"
BASE COUNT         2002 a         2067 c         1824 g         1899 t
ORIGIN      
        1 tgttttccgg attccagcta caaaaaagtg aaaaaaatgc aatttttgct ttgcgctttt
       61 tgagattttg ttttaaaaat tgttcaaaca cgcttaacaa aaagcttctg ctcatttgct
      121 cttagaagcc atacttcgaa cgtatgcatg gcacagcaag tttgtgtcca ttaggagcag
      181 cgaagactgc ttcttctccc ccttccttgg gtttgcgaaa taaggcaatg aataactctg
      241 gccatactgg ccggacgttg gtgtacggct gctttccagt cgggctgatg tcacggtctt
      301 gtatgccgga aatgttttcc gcgtattcag ggaaagagta agtgctgcta tgaaaaaaag
      361 tagtgcgaaa tcttttcaaa tgacaatccc ccaaaccttg tattcaaagg ctgtcttatg
      421 aagttgagat tctttttcat cgcacttgca tgttgtttgc ttgtggtatc gttccatcgc
      481 gcagaagccc aggcgagcgt gacagaaaac cagtctattt atctgcatgt caacggaact
      541 accgggtctg attccaatcc cggctcgccg acacaacccc tcaagaccat cggtgcggct
      601 gtggcaaaag cactggccaa caatcgcgct ggtatcggca ccaaggtaat gattgatcct
      661 ggtgtttacc gtgagttcgt caagatcagt ccatacaatc aaaccagcgc accaattacg
      721 ttccaggcca caacagcagg aacggttaag attgatggcg cgaatgtgct gacaaactgg
      781 tacaggaaga acaccaacat atatgcgtac tcatggaaag acccggtagg tggctgcccg
      841 cttcccagtg gctggtatac cggaatgccc tacatcgtac aaaacaatga gatggtgttc
      901 gtcaacggcc aggcaatgac gcaggtttcg tctgtttcac aaatgcgtgc cggcacattt
      961 tttgtgaaca gtacgtatca ggagctggac gtttggccgc cttcaggtac agacatgaat
     1021 acggccactg tggaggcttc cgcgcgcaga gaaacgcttg aagtggacaa tggtaaaaat
     1081 atggtgtttc gcggcctcat tctgcagcat gcagcttcct gcatgaacca gaatggcgca
     1141 aatgtgtata acagctcgaa tattctcttc gacaatgttc aggtgtactg gaacaactgg
     1201 ggaggattgg gcatcagcgg cagcaaccac tacaccgtgc agaactctac tgcgaactac
     1261 aacggcggag tggggctcgc ggcctatgag gtaaaaagcg ccttatacca gaacaatgag
     1321 acggactaca acaactggcg cggcgagatg ataggtctgt acgattttgc ccagggcggc
     1381 acaaaactga tgcatgctca tggaatcaca gtcaattctc accgctcata caataacgct
     1441 gcccagggac tgtggttcga tactgacaac acgaacgtca cagttgccaa tagccagctc
     1501 gttggtaata cggtggccgg tctccaactg gaagccaatc aggggcctgt cactgtgcag
     1561 aattccagca tgtgctccaa cgggatgggt gtacatctga ttaatacgaa cgacctcacc
     1621 atgactggca atgtcttcta taacaacggt ggtcagaatt tccagaatgg caatttcttc
     1681 cttgccggaa aggctggcgg ccgcgtcttc accaactttg aaaccggtca gacagaaact
     1741 actcttacag aaaatacaaa gcttgaaaat aacgttctca ctgccgccaa ttccagtcaa
     1801 ttcgtcttca atacttatct cagtggcagc gactggtcaa cgttcattga tagtctgcag
     1861 tcaaacaata actactggta caacgcacaa cagccatctg cttatattgt gactctgggc
     1921 aagcgtgtca cttttggcgg ttggaaatca gtttccgggc aggactataa ctcttcctgg
     1981 tcacaagcat ctacttatgc ccgtggctgt ggggtcccca gtccttccta cacagacttc
     2041 catgtccagg cacataacgc tgccgcatac gtgcctgcgt actacatgtc caatggatca
     2101 gtctccattc cgttgcaggt aaaatcttac ggctatggat cggtccagct ttcagtctat
     2161 ggacttccct cgggcgtcag cgcttcgttc agcccctcta cactgaccag cgggaactct
     2221 actctcactt tgaaggcatc ttcctctgcg gtagcgcaga ctgtaccgat tacggtattt
     2281 gctgcaagcg gaagccgcgt ccattccatt actttgaagg tgggcatccg gccggcgtct
     2341 tagtctgtgg atgcgcacta ttcctgtcag gccgtggtca gttgaccgcg gccttttttc
     2401 ttatgacccg agcaggaatt gtatctgtcg caatgtatag gcacaccgct tatgctggcc
     2461 ttctgtccag tcctcaaaga atgtacgcag gaggtaatag cttgtaagac ctcgcagtgt
     2521 ctcttttgag agcctgcatt tgcgcgcata tgtttgtagt aaagcgttgg acgttaatat
     2581 gtcccagaca ttttttgatt cgttgaagag atagtcctga atataaaaat agcggcagat
     2641 gtcctgccat ggcaggctgt tttctaccgc ccactcccag tcaatcaggg tgatttttcc
     2701 atccgcaaga agccgtatgt tccatggagc gaaatcgcgg tgctcaataa atgccatcag
     2761 ctcttcgtcg ttttccagca aagagagggc ctgttcgatc ccttgcattt cggttttggg
     2821 gcggtcgaag cagcttagtt gcgttgcgag cgcattgcga aaatcagaca gcctgatagt
     2881 gcatccattg catccaagcc ccagaagaaa atcaaggtgc tcttccctga acctgcgcga
     2941 tatgtgcctg ccttgcatcc aggattgggc caccacccca tgttcctcat cagtgaacag
     3001 gacctcagga acaatgtact gtgcttttac tcttctcaga atggccgctt cattcagaat
     3061 ggcggctttt gcctggacgc ttagcggaat ttttgcaact gcccggacgg aatccttgtc
     3121 cagaaaaaag aaaatcgctt tctgcgtggg tgatgggttc ccaacatgcc ccagtatctc
     3181 ccattccgcg gaaaggccat tcagttgggt cttccagtaa ggaagattgc aattcatttc
     3241 gaccgttttg acctcgggga gcagcggcaa gagattcatt gaagatgccg ctatgactcc
     3301 atgccactgc aaacgcgatt tcgtaccata gggtctccag cttttaaata cagtcaatgc
     3361 ggagcgattg ttctcaggca caatccatcg aggctgatcg ccctgagaaa taacatgaaa
     3421 ttttcgctgc ggaagtccga ctgcatcaac cgcggctgga aagcggaaaa tactctccag
     3481 aagactttta gaccgcaaaa cctcgtccgg accatcaagc aggtttgttt ctgttttgac
     3541 cgcgccgttc aagcttctgc tccctctccc aggcccagac gaatgcgggc ccgcttggaa
     3601 taacaggaga caattgcctc agccgtgtca tgaacaacat ccgaaagagg ctgtgcagca
     3661 tctatcacga ctgcatgctt caacttcacg gcaagctcta aatacatctt ctgctgacgg
     3721 cacacttctt caaaaggcac ttcctgcttg cgagaccaaa ggacttccgg agacgcgttc
     3781 agcagaatga ccagatcagg cttcggaagc attacagcaa tccatcgcag gagccatctg
     3841 ggtcctccat aacgcacccg tttagggtcc accagaatgt cgtaaaaata ccggtcgaat
     3901 ataaccagct ttgtccgcat catggctggc aagacccgca taaaatgtcc taggaaataa
     3961 tcaaacgcca gatagagcat cttggccgcg gaagcaaggc cgctgcgtga aggcttgcca
     4021 tgtggatctg taaccactga accaggtttt atccgcccag gaaaaattcc gggccgcatg
     4081 tgacaacgta caatctgctg aaatgccgga ccgaattcgc ggatgaccgc ttcgatcaca
     4141 gaggacttcc cgcaaccgtc cggcccgaca aacacgatcc atccacccgt aggttgaata
     4201 attcgctcga aagtatgcgt cagatttctc cagcgcgaaa ttgcccttcc cttccagcct
     4261 tcagcgggat gttgcagcat ctcttccttg aacagtccgg ttttctgaat gagaggccgc
     4321 cagtctcccg tacctgccat cttcgatatg cgctgtgcag tgtcctccgt ccagaagcgg
     4381 cgcaacagta catcacagtt tgcaggatct tctgagtaga gccgcgacag ccggatacca
     4441 tgttcctgcc tgaaatcacg cttgtttaac cgcttgatca ggtagtacaa aaattcgtgc
     4501 cgcgcggccg gaatccaaaa tcctttcgga tgtggtcgcc gggcttccag aacttcatca
     4561 gacttcagcc ataatttgcc aaagtgccgg tagtctgagc aggaatccgg ctgaatgaaa
     4621 gtagttccat ctgccgtgag atgcgccaga cgaaaggcgc gggctgtagc ttcatgcggt
     4681 atgacttgaa aaagccgcgt gcctgtttgc tgcgcaataa aggcaatcca ttccggcacg
     4741 cgctcaaaat cctgcgcgcc caccatgaaa tcaatatcgg aatcaaaact ctcgggcaga
     4801 tcgtcgtagc ctgtcagaat gcaatatgca ttgctgtttt tggaaagctc ttctattaag
     4861 agacgggcca cgagcgacgg gacagtagtt aaaccgccat cgttcgccat attgtccttt
     4921 tggacttcca gcaacttacg cctccctatt aacaacaaat cctgaaacag ttccgttccg
     4981 gctcatccgg aagatggtac atttctttaa gaccatagtt gatatttgga gtaaccagga
     5041 aagaatgaag attctcctct ctgcctatgc atgtgagccg cataaaggct cagaacctgc
     5101 ggtaggctgg aattggatgc tttctcttat cgggcaaggg catgaggtgt tcgtgcttac
     5161 ccgtaagaac aatgccccgg ccatcgaatc tgaaattaag aaacagcacc tttctgttac
     5221 tcctgtttac tatgatcttc cctcctggtg caggcgatgg aaacactggc caggtggctt
     5281 atatttttac tatcttttct ggcagttggg tgcatatttt tacgctaaac agcttcacaa
     5341 agtacaacgt ttcgaccttg ttcaccacat aacatttgct acttttcgcc aaccctcttt
     5401 tatgggaaga ctgggcattc cctttgttct tggcccggtg ggcggaggtg aaacatcccc
     5461 acgccatctg cggcgcggct tcaactggtc gggccgtctc cgcgaaacgc tgcgggacat
     5521 cggaatcgca tgggcaagtc acgatcctct tatgcactcg accttttccc atgcaagttg
     5581 gatagcgtgc acaacacatg aaaccttgaa gcaaattccc gggcgctatc actccaaatg
     5641 catcgtgcaa cccgcaattg gcatagattc gcctcaattg tcctcttcaa tttcacaacc
     5701 atctcgcaac aatttcctct tcattggccg cctcctttac tggaaagggc tgcaccttgt
     5761 actgcgcgca tttcctgacg tcctacagag gatccccgat gcgcgcttgc gcatcatcgg
     5821 cgaaggcgaa gactcagact ggctgaagag tatcgcccag gaatgcggca tctcatctgc
     5881 tgttgactgg attccctgga tgccacacca acaaatagcc gcggaatacg aaaacaacat
     5941 cgcctttgtc tttcccagcc tgcatgattc aggcggtctt gtcgtgcttg agtcccttgc
     6001 cgcttcattg ccggtcattt gtcttgatct gggaggcccg gctatttttg tagacgattc
     6061 ctgcggcatt gttatcagca catcggaacg aaacgaagcc tctgttcaaa aaacgctggc
     6121 ccaggccatg atttctctcg cctctgatcc ggaaagacgc aacctgctcg cttccggctg
     6181 tcaacaaagg gtccgccagt ttagctggca ctccggtgca gaccacttat actcttttat
     6241 aagttctgaa cattctcgct agctacttac acctggctga tttctacctc tgtacttatc
     6301 tgctccttct ttgctccatt ggaaactcga aagcggttcc aggcaagaaa caggaagact
     6361 agatttgcaa tttgccctcc aacagccgca accataattc ccatggcctg aaaactgcga
     6421 atacagggat aaataagaac aatacttgta atggctgctg ctaatgaaga agtaaggatt
     6481 gcagaagtct ctccaattgt ccgaaacaga tagctgaact gccgcagagg ccacaacaaa
     6541 agaaagtaca acatctgcag attaagaatt ccagcataga cagccacttt gtgcccataa
     6601 aaaaaagcca gaaattcccg ggagaaaaat gatatcccta aaaatacaat agatagcgca
     6661 ataaagccct tggtactgaa caacagcaga aaacgcgaga ccctggtaat ccctcccttt
     6721 ctcatttcct cgctggcctt caaaggaaca atgttttcca ttgcctgata agcaacattc
     6781 acagggccga ccacgttttg tgtggcccgg acgcttcctg cggcttctga tccgaggacg
     6841 cttgcgccaa tcagcatcgc cccttgatac acaaaccact gcaattgatt ggcgattgca
     6901 agatcacgtg aaatcccgcg agaacgcgcc catgactgct tcaactcgtc tccagaccaa
     6961 cgaagacgct cgattgcggc acctgctccg aatgcaatgg ctgaagtgac cgcaatcgcc
     7021 agaaacgcgg tgtttacagt tagacgatgt acatacatca acccgagtag cactatcgtc
     7081 tggcccagat aacttataag gtcgttccat acagagtgga aggacttatt cacagcgaag
     7141 taatagcgcc gcagccagtc ctgcatcagg tagaaaacaa ggcacagcag gaatgaaggt
     7201 agttgatcca gtcttgcaaa tcccggttta agccacaaaa aaattgctgt aatgaagaaa
     7261 gtaagagcag tcgcaactgc gcagaatttc acctgaacgc caaacatccc gcgcaggtag
     7321 cgcatccttg cttcttcatc cttgcacagt ccggccaatg taatcattgg agaggcgata
     7381 aaacttaact gaacactgtt ggcgaggagt acaagagcat aagcaaccgt aaaaacacca
     7441 aactgttcaa gccccattcc gcggaccaga ataattccag ttgcaaaatt tgagccgctt
     7501 accacaagct ggtcagcaat ggtttgataa cgtcggcggc gtaagaattg catataaaat
     7561 agattcattc tttgtccaga ttcatagagc acactctgtt tcagcttgct catttcgcaa
     7621 cggccaccag acctggcgga gcggcgactc ggaccacacg ctggtacgca taggcaggcc
     7681 ggtgccggac aagatcaagg ttcgtcatga tgcaaaggaa cagggccatc tcatatccca
     7741 tccagcccag gacgcctgtt gacatcaccg tagtcatcca gacgtgaatg at
//
LOCUS       DASRBV010000227         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_472907, whole genome shotgun sequence.
ACCESSION   DASRBV010000227 DASRBV010000000
VERSION     DASRBV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472907"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFA02_08515"
     CDS             <1..1086
                     /locus_tag="VFA02_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU09935.1"
                     /translation="GDYEVGSIKSIDLTTRTVYYVSNEGDARQQQIWSVKLDGSEKKK
                     ISEADAFHDPDFAEGTSTYFDHASSLMTPPSFSLCHAGSSCQTIWQGKMPDGYELVKP
                     EMLELKAADGKTTLYASLLLPKNATTSGSVPLINNPYGGPHAQTVRDSWGGGMFFFDQ
                     LLAQRGFAVLHVDNRGMGGRGRDFEQAAYRNFGPVQLSDQLAAIDQVLAKYPQLDKDR
                     LGWWGWSWGGTFTLNALSHSDRFRAGVSVAPVTDFRNYDSIYTERYLGLPDQNKEAYD
                     AASELESAKNLKGRLLLIHGTGDDNVHFANSIQYIQRLIDAKVPYDFQPFPRKTHSIA
                     GPVAHTFLFQRMLWHFDTYLKPAGAQQ"
     gene            1083..1976
                     /locus_tag="VFA02_08520"
     CDS             1083..1976
                     /locus_tag="VFA02_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU09936.1"
                     /translation="MSHPAERANSHELVRGRTTLGDFELTVLSDGFYFLDGGAMFGVV
                     PKTMWEKRAPADEQNRILLGLNTVVVRTGKQILVIETGIGNKLTDKLKQIYGAQEKLP
                     AAFAAAGIKPEEVDIVINSHLHFDHCGWNTTRTPNGCIVPTFPNARYFAHRGEVEHGH
                     LQLERDAISYISENYDPLVENSQMTLLDLKRGETRELAPGISVELFPGHTSQLIAVHL
                     ESGGKHACYISDLIPTSAHLDITWVMGYDLDPLTCIEERKRFYKRAIPEQWLVLFTHD
                     HHTPFGYIEWNEKGKPEIKAD"
     gene            complement(1989..>2343)
                     /locus_tag="VFA02_08525"
     CDS             complement(1989..>2343)
                     /locus_tag="VFA02_08525"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZU09937.1"
                     /translation="RTFGFKEDEQMLRNMGLIRGVSESSAIILTRQGVVNGPLRFEDE
                     FVRHKILDLIGDLALAGRQILGRVVAERAGHAMHTALVSKLLRDRSAWKLVTLASEKA
                     KVKSVTVGATSLATA"
BASE COUNT          542 a          671 c          625 g          505 t
ORIGIN      
        1 ggcgactatg aggttggctc catcaagtct attgatctga caacgcgcac tgtgtactac
       61 gtctcgaacg aaggcgatgc gcgccagcag cagatctgga gtgtgaagct cgacggctct
      121 gaaaagaaga aaatctccga agcagacgct ttccatgatc ctgatttcgc ggaaggcacc
      181 tctacctatt tcgatcatgc ttcttcgctg atgacgcctc cttctttcag cctgtgccat
      241 gcaggaagct cctgccagac catctggcaa ggcaaaatgc cggatggata cgaattggta
      301 aagcccgaga tgctggagtt gaaggccgcc gatggcaaaa ccactctcta cgcctcattg
      361 cttctgccta agaatgcgac cacatccggc agcgttccgc ttatcaacaa tccttatggt
      421 ggcccgcatg cgcagactgt acgcgatagc tggggcgggg gcatgttttt tttcgaccag
      481 cttcttgccc agcggggttt tgctgttctg catgtggaca accgcggaat gggcggtcga
      541 ggccgcgact ttgagcaggc agcctatcgc aactttggcc cggtgcaatt gagtgaccag
      601 cttgcggcga tagaccaggt gctggcgaag tatccgcaac tggacaagga ccgcctgggt
      661 tggtgggggt ggagctgggg cggcaccttt acgctgaatg cgctctcgca ctccgaccgt
      721 ttccgtgccg gagtttcggt ggcccctgta actgacttcc gcaattacga ttcgatttac
      781 acagaacggt atctgggcct gcccgaccag aacaaagaag cctatgatgc tgcatcggaa
      841 ctcgaaagcg caaagaacct caaaggtcgc ctgctgttaa ttcatggtac cggcgatgac
      901 aacgtgcatt tcgccaattc catccaatac atccagagat tgattgatgc gaaggttcca
      961 tatgatttcc agccatttcc gcgcaaaaca cactccattg ccggcccggt agcgcatacg
     1021 tttctgtttc aacgcatgtt gtggcacttt gacacgtatc tgaaacccgc aggagcacaa
     1081 cagtgagcca tccggcagag cgcgccaact ctcacgagct ggtgcgcgga cgcaccactc
     1141 tcggtgattt tgaactgact gtcctgagtg atggcttcta cttcctcgac ggcggcgcga
     1201 tgtttggcgt ggtccccaaa accatgtggg agaagcgcgc tcccgccgat gagcagaacc
     1261 gcatcctgtt ggggctgaac acagtcgtag tgcgcacagg aaaacagatt ctcgtcattg
     1321 aaaccggaat cggcaataaa ctcaccgata agctgaagca gatctacggc gcgcaggaaa
     1381 aacttcccgc cgcttttgct gccgcgggaa tcaaaccgga agaagtggac atcgtcatca
     1441 attcgcatct ccacttcgac cactgcggct ggaacaccac gcgcactccc aatggctgca
     1501 tcgttcccac gttccccaat gcgcgctact ttgcccatcg cggcgaggtg gaacacgggc
     1561 atctgcaatt ggagcgcgac gccatcagct atatcagcga gaactatgat cctctggttg
     1621 agaacagcca gatgacatta cttgatctga agcgcggcga aacacgcgaa ctcgctcctg
     1681 gaatttccgt ggaacttttc cccggccaca cttcgcagct catcgccgtg catctggaat
     1741 ccggtggaaa acacgcctgc tatatttcgg atctaattcc aaccagcgcg catctcgaca
     1801 tcacatgggt catgggttac gatctcgatc cgctgacatg cattgaagag cgcaaaaggt
     1861 tctacaaacg cgccattcca gagcagtggc tggtgctttt cacgcacgac caccacacgc
     1921 ctttcgggta tatcgagtgg aatgagaaag gcaagccgga gatcaaggcc gattaaacct
     1981 tggcagacct aagccgtggc cagcgacgtt gcacctacag ttacggactt tacctttgcc
     2041 ttttctgaag caagagtcac gagcttccac gcggagcgat ccctgagtag ttttgatacc
     2101 agtgcggtat gcatggcatg tccggcacgc tcggcgacaa cgcggcccag aatctggcgt
     2161 ccggcaagcg ccagatctcc gatgaggtcc aggatcttat gacggacgaa ctcgtcttca
     2221 aagcgcagcg gcccattcac gacaccctgg cgggtaagaa tgatcgcgct cgattccgaa
     2281 acaccgcgga tgaggcccat gttgcgcagc atctgctcat cttccttaaa accgaaggtg
     2341 cgt
//
LOCUS       DASRBV010000228        12943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_524137, whole genome shotgun sequence.
ACCESSION   DASRBV010000228 DASRBV010000000
VERSION     DASRBV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524137"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3033
                     /locus_tag="VFA02_08530"
     CDS             <1..3033
                     /locus_tag="VFA02_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09938.1"
                     /translation="SVDAIQEFKVVTNPYDAEYGRTSSGVVSMELKSGTNQLHGSAYE
                     FAKRGFLDANSWENNYVGQNRPAHTEDQYGFAVSGPVYLPLLYNGRDKTFFMFNFERF
                     KEVLPTFQTYDIPNAAWLQGDFSNFVDQGGAMMPVFDPSTATTADPTRQIFHNSAGQY
                     NRIDPGRFNPVAAKIVGMMLQGAQATSLRFPNELPWEEIWIDTIPQTASSQNFVFKFD
                     QVLGAHDHLSGNWVRSRNPSFAPDTPPNVPWFNGSTFTEYHMNTGLDWVHTFTTNLLT
                     DLRISYQRYWRTDGPTAANLNFDPSQLGLPSSLLSELPLKKGFPIINFNMQQQSVGTG
                     NGYNNWLAMSRDFYYMPDDSYNLAPSVTWNHGKHNLRAGLDFRISHLNQTLQYENIIS
                     YSSNGIATSEYWDQNDTNDIAYAPDGTPLSQNGSGNAVLDFLIGQPNSAAVQNQLFPY
                     YTWHYYAPWIQDDWKVTPKLTLNLGFRYDFNGPPTARHNWLNTGFDFNAVNPVNDLVS
                     HAGGLPTLKGGILFPATSHTNQPWAQDYTKWQPRLGFSWQPIQGTVLRGGAGRVVMSP
                     ANNPQTAGFTNNPAFNNSPDGGRTYFPDNIGNPYPGGIPPIPGASLGLLTYLGKSVFF
                     ANPHYKLPSVINGSLGIEQAMPHDGKLEVSYVMSRGYGMDVEYTALDSNFALYKSCNA
                     LQSTASNPYPQGQCQGLAPNPFYNVPGVAGSLGANPQISKYQLARPYPQFTGINELQR
                     NWGHSWYNSLQTSYQQRLGWEQINATWTYSKTMQSGGYRDDIYLIPVHTIAGTDRANR
                     ITVTSVMNIPIGRGMKYFSGMDRWLDAAVGGWELAANGFWETGQPVQIDGNSQSYNVI
                     GDIKSHRPRQNTPDILDAGVNPCTEKWHGSSPTKPGYYTLMEYGQEVPSCSNGLQPAW
                     QEVAPYAPKTSQEWTDQVRGPGANQIDVNLSKNFKLTERLSLQLRMEEFNVLNHPNWY
                     GQVDMTPTDTNFGTVNKILTGGQSNSPRQGQLGAKLTW"
     gene            3505..4266
                     /gene="agaR"
                     /locus_tag="VFA02_08535"
     CDS             3505..4266
                     /gene="agaR"
                     /locus_tag="VFA02_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor AgaR"
                     /protein_id="HZU09939.1"
                     /translation="MLIEERRQHILALVQSQGRVLVNELSESLGISQITIRKDLDYLQ
                     AKGLVQRTHGGALPLSAGAMFDPSLQEKMRQHAQEKQRIANAAAKLVQEGQCVMLDSG
                     TTTTAVAHALRRFAHLTVITNAINIAADLAGTDFDVILTGGTLRKNSFSLVGPLAEDV
                     LDEMHADVLFLGVDGFDLEIGLTTPNVLESRVNRAMVKAARKVVVVCDSTKFHRRSLS
                     RIIPPSDVHCVITDRGLSETDAEVLRGLGMELVLV"
     gene            4333..5286
                     /locus_tag="VFA02_08540"
     CDS             4333..5286
                     /locus_tag="VFA02_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZU09940.1"
                     /translation="MFDVTIAGEINLDLVLYGLPRNMPTERELLASDFQATLGSSSAI
                     LAHNLAVLGANVGFITRVGTDELGKIALERLTASGTDMSRTTFDTKHSTGITVLLHHG
                     KERHILTYPGTMREMTVRDLDIAYLKSSRHFHLSSLYLQTGLAPDLPGLFRELKRAGL
                     TISLDTNDDPEDTWGGVLDELLGLIDLLLPNEAEARRIAKRNTLEESLAVLSQRVPCI
                     AVKRGAQGALVQVGNTRLTAPAVRVTPIDTVGAGDSFNAGFLFAWLRRLPLDACARAG
                     NITGALSTLRPGGTEAFRDRELMITFLKQHNFPAAVAETKA"
     gene            5286..6803
                     /locus_tag="VFA02_08545"
     CDS             5286..6803
                     /locus_tag="VFA02_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZU09941.1"
                     /translation="MNFAPVDWTIVVAYLAASLLIGLLGRKYVGNTTHFLVAGRELGT
                     YIGIATLAATEIGTITFMYNSELGYRYGFAAFSAALISGIAMIFVGRTGFVIRRLREL
                     RLMTVPEYFEVKYSRGLRIVTGFLVALGGILNMGVFLKVEGEFLTVVSGLDQKYLVVT
                     MIAILLLELIYTTVGGMISIVITDYLQYVLLSVATIIITIYAVHFAGWHAMVAKVTTV
                     MGSSGFNPITAPKFGWSFLIWQLLLWLSIHTCWQTTAMRMFSTRSPETSKRVMTWTGF
                     IFLGRGMLPMIWGIAALTLLGHGALTGGVPGPIAHGHSLAPIDAMPAMLAMLLGPGIR
                     GIVVAGMLAATMSVNSSYLLGWSAVISQDIIGPMRKQMGRPPLADATQLLINRICNFC
                     VGLFLIFWGLLYTPPGAVYLYLNITGTIFLAGAFICVIGGLYWKRANITGGYCAMVLG
                     AAGAIVPFFFLHKSESFAGFAAFGLSAVGLLFGSLFGKSTKVPQPQMAIPEGEAR"
     gene            6800..6946
                     /locus_tag="VFA02_08550"
     CDS             6800..6946
                     /locus_tag="VFA02_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09942.1"
                     /translation="MNAWICFWAVWLVISGAAFAIITLIVAVKGFEDLRAMFAGLSGE
                     KKKS"
     gene            7071..8120
                     /locus_tag="VFA02_08555"
     CDS             7071..8120
                     /locus_tag="VFA02_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU09943.1"
                     /translation="MSNQDKFVAMVTARAEVDLPLSKFQPKSMVVAEEHLPERPRFPA
                     IDYHNHLDALEPRDVLRVMDACGIEKVVNITMKTGEAALAMIDKFHSAAPDRFATIGW
                     MDWSDVTHPDFIARSLERLERLAERGAKGIKFWKDLGLSVRDASGHLLRVDDERLAPL
                     FDKAAELGIPVMFHIADPAAFFLPIDEHNERYEELAAHPDWGFYGSQYSKWELLDQRD
                     RVIARHPKTTFVCAHVAECGENLARVTKLLESCPHVYIDISARASELGRQPYSARELF
                     LRFSDRILFGTDLLPEETMYRLYFRFLETADEYFEYPSHASRQGRWNIYGLYLPDDVL
                     RRVYRENALRLLNTA"
     gene            complement(8122..8928)
                     /locus_tag="VFA02_08560"
     CDS             complement(8122..8928)
                     /locus_tag="VFA02_08560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU09944.1"
                     /translation="MKSPKLLREAFQGRWALVTGASAGIGLALAEQLAAAGTNLVLTA
                     RRKERLEATASRFEGQYGIQTCVVAADIAHPSAPDALFAATEGAGLVVDVLVNNAGFG
                     HYGRFAEGDAAWQRRMVEVNCAAVVHLTRLFLPKMIERRRGYVMIVASTAAYQPVPYM
                     STYAATKVFDRFLAEALAHEVAEHGIKVSALCPGPTESEFHEIAGTRNQFGRGRQSAE
                     EVARIGLEGLARGEHWVIPYRAGKAMVLIERLLPRRFVSGMAAKVFRPKA"
     gene            8956..9354
                     /locus_tag="VFA02_08565"
     CDS             8956..9354
                     /locus_tag="VFA02_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HZU09945.1"
                     /translation="MLARPGILLRAEALLVLVASCAGFSMLHGSWLIFALLFLAPDIS
                     LLGYMARNSLRIGTTLYNAAHSYIGPAVLGLLSWRLHSPRFGQLAAIWIAHIAFDRLL
                     GYGLKFPNAFKPTHIQSAAIFAGTPRQSTG"
     gene            complement(9317..11299)
                     /locus_tag="VFA02_08570"
     CDS             complement(9317..11299)
                     /locus_tag="VFA02_08570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZU09946.1"
                     /translation="MAESVSVVATKVTRPWEGAADETGVESWVNAALERHKEALEQLL
                     AVSGLRTVENTLSAYDSAVAELSMARSQTALLNSVHPEKKVRDAAQTLIQKVAEAGTL
                     LALNQEVYRALEAMDLSGADSGTRHYVERTLLQYRLAGVDKDEKTRARLRELHDKATH
                     LSLVFRRNVQENVNKVVVEDKAELEGLPEDFPANHPADEHGQITLTTDFPDYLPVMTF
                     ARNPKLRLQMFLAYNTRAYPANEGVLLDLLKTRREIANLLGFETWADLATADQMMGSS
                     KELRAFLDGLDEATKAGAEREYAAVLEFARQYEPKIVAIDADSRSYWYEQYRRSAYDF
                     DSQTVRPYFPYERVERGVLETAQKLFRVSFRPAEDAEVWHESVGAWDVFDGGKRIGRF
                     YLDMHPREGKDKWFSAHPLIPGIAGRQIPEAALVCNFPEPKDGNPALLQYSDVVTFFH
                     EFGHLMHALLGGKQKWAGISGIATEDDFVEVPSQMLEEFFRDAKLLATFAKHYQTDEP
                     IPAELVEKMNRAGAFGRADWVRTQLFYTTYSLDTHYEDPATLNLDVLLQADYTKFLPY
                     TWIDGNRMYASFTHLTGYSSNYYTYLYDKVIALDFFSQFNREDLLDDPVAMRYRKAVL
                     EPGGSLPGKEIVRQFLGREQSLDAFTQWIGEEFQQK"
     gene            11436..>12943
                     /gene="aroE"
                     /locus_tag="VFA02_08575"
     CDS             11436..>12943
                     /gene="aroE"
                     /locus_tag="VFA02_08575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZU09947.1"
                     /translation="MNQTVPAISPQFIRSRIGKVCVAIIGSTPAEMLEKAQETIRENT
                     FLEFRLDYLDKPLAAVPKLKQFLAERPDVTAIATCRRVAAGGKFKGKIADELDILSKA
                     AAAGFHLVDVELQTAENVKEAELEKLRARGAALIISWHDFAATKDLDGIFERIRPYEP
                     DFIKIVSTAKNLSDNVTMMHFLERTRDMANMIGICMGDQGIISRVLGIRAGSVFTFAA
                     ATPGEETGPGQIAARTLRETYRIDQIDAATKVYGVAGNPISHSLSPLMLNTAFRRETV
                     NGVFLALQTAKIKDLLTLVREVPIHGLAVTMPLKEEILEHLEKTDPVSEKIGACNTVV
                     RAQDGKLYGFNTDVAAVVRPLERRLQLRGAKVLVLGAGGAARAAVFGLKEKGAEVFIL
                     NRTAADGQKLARQAKARTFRRDQLAKTQFDIIINATPVGMQGVKPNHILEPKEINARL
                     VFDMVYNPIETPLLRMAREKGIPVVTGVEMFVHQGARQFEIWTGKPAPEEEM"
BASE COUNT         2838 a         3975 c         3303 g         2827 t
ORIGIN      
        1 tccgtagatg ccattcagga attcaaggtc gtcacgaatc cctatgacgc ggaatatggc
       61 cgtacatcca gcggcgttgt cagtatggaa ctcaagtccg gcaccaacca gcttcatggt
      121 tctgcttatg agttcgccaa gcgaggattt ctcgatgcca atagctggga aaacaactat
      181 gttggacaga accgtcccgc acatactgaa gatcaatacg gttttgctgt ttccgggcca
      241 gtctatcttc ccctgctcta taacggccgc gacaagactt tcttcatgtt caatttcgag
      301 cggtttaaag aagtattacc aacgttccag acctacgata ttccgaacgc ggcatggctc
      361 cagggcgact tctcgaactt cgtggaccag ggcggcgcga tgatgcccgt ctttgacccg
      421 tctaccgcta caacggctga ccccacccgt cagatattcc ataattctgc cgggcagtac
      481 aaccgcatag acccgggccg ctttaatcct gtggcagcca aaatcgtagg catgatgctc
      541 cagggtgcac aggccacttc cctgcgcttc cccaacgagc tcccctggga agagatctgg
      601 attgacacaa tcccgcaaac ggccagctcg caaaactttg tcttcaagtt cgaccaggtg
      661 cttggcgctc atgaccacct ctccggcaac tgggtccgca gccggaatcc atccttcgct
      721 cctgatactc caccgaatgt cccctggttc aacggcagca cctttaccga ataccacatg
      781 aacacaggac tcgactgggt gcataccttc acgacgaacc tgcttaccga cctccgcatc
      841 agctatcaac gctactggcg cacggacggg ccaactgccg caaacctcaa tttcgatcca
      901 tcgcagcttg gcttgccttc ttccctgcta agtgagttgc cgttgaagaa gggcttcccg
      961 attatcaact tcaacatgca gcaacaatcg gtcggcaccg gaaacggata taacaactgg
     1021 cttgccatgt cgcgcgactt ctactacatg ccagatgaca gctacaacct cgcccccagc
     1081 gtcacctgga accacggcaa acacaatttg cgcgctggac tggatttccg catctcgcac
     1141 ctcaatcaga ccctccagta cgaaaacatc atcagctact ccagtaatgg aatagccaca
     1201 agcgaatact gggaccagaa tgacacgaac gacattgcct atgcgcctga cggcacgccg
     1261 ctctcacaga atggcagcgg caatgctgtt ctcgattttc tgattggcca gccaaatagc
     1321 gctgcagtcc agaaccagct cttcccctat tacacgtggc attactacgc gccatggatt
     1381 caggacgact ggaaggtcac tccaaaactc actctgaacc tcggctttcg gtatgacttc
     1441 aatggcccgc ccaccgcgcg ccacaactgg ctgaacaccg gctttgattt caacgccgtc
     1501 aatccagtca atgatctggt aagtcacgca ggagggctgc caacgctgaa aggtggcatc
     1561 ctctttccag ctacgtcgca caccaaccag ccctgggcgc aggactacac caagtggcag
     1621 ccgcgactcg gcttctcctg gcagccaatc cagggaacgg tactgcgtgg cggtgccgga
     1681 cgcgttgtca tgagtcccgc aaataatccg caaaccgccg gatttaccaa caatcctgcc
     1741 ttcaacaact ctcctgatgg cggccgcacc tatttccctg acaatatcgg caatccatat
     1801 cctggtggaa ttcccccgat tccgggtgct tcactcggtc tgcttaccta tctggggaaa
     1861 tcagtctttt ttgcaaaccc gcactataaa ctgccatctg tgataaacgg atcactcggc
     1921 atcgaacagg ccatgccgca tgatggaaag cttgaagtga gctacgtaat gagccgcggc
     1981 tatggcatgg atgtggaata taccgccctg gactcgaatt ttgcgctgta caaaagttgc
     2041 aatgccctgc aaagtacggc atcgaatccc tatccccagg gccagtgcca gggtcttgct
     2101 ccgaacccct tctataacgt cccgggagtt gccggaagtc tgggagcgaa tccgcaaatt
     2161 tcgaaatatc agcttgcccg gccatatccg cagttcactg gaatcaatga attgcagagg
     2221 aactgggggc actcctggta caactcactg caaaccagct atcagcaacg cctcgggtgg
     2281 gagcagatca atgccacctg gacctactca aagaccatgc aatccggtgg ctatcgcgat
     2341 gacatttacc tcattccggt acacaccatt gctgggaccg accgcgccaa ccggatcact
     2401 gtcacaagcg ttatgaacat tcccatcgga cgcggtatga agtatttctc cggcatggac
     2461 cgctggctcg atgcagcagt tggaggatgg gagcttgccg ccaatggctt ctgggaaacc
     2521 ggccaacccg tccagataga tggtaacagc cagagttata acgtgattgg cgatatcaag
     2581 tcacaccgtc cgcgtcagaa cactcccgat attctcgatg caggcgtgaa tccctgtacc
     2641 gaaaagtggc acggctccag ccccacgaag cccggctatt acactctgat ggagtacgga
     2701 caggaagtcc cctcctgctc caacggactg caacctgcat ggcaggaagt ggctccatac
     2761 gctcctaaaa ccagccagga atggacagac caggttcgcg gcccgggagc caatcaaatt
     2821 gacgtaaatc tgtcaaagaa cttcaagctc acagaacgcc tcagcctgca gctccgtatg
     2881 gaagagttca acgtactcaa tcatcccaac tggtatggcc aggtggacat gacgccgacc
     2941 gacaccaatt tcggcactgt aaacaaaatc cttaccggtg gacaaagcaa cagtccccgg
     3001 caaggccagt tgggcgcgaa gctcacctgg taacgaatcc gcttctcccg ggcgtccaat
     3061 tgcctgcatg gtgttgggcg ctttttcttt caggaaccaa aatgaaaata cttcttcttt
     3121 cgcaagactg gccgggttcg gcaatcagtg gatcaaaaca acggacggtc catggatcaa
     3181 gctgactaag gcccgcttta cgcatactgc gcgcgtcatc tacaaagacc gcctcgaccg
     3241 cggcgtatgt aatcactggc aaccgctttt atctcaccaa tggaggattc aagccacaat
     3301 ccattgagta caacaacgaa atcagccgtc ccgccaatgg ccacactccg gaaatcactc
     3361 tgctccaggc aaaggcccaa tagaagccgc tgcttctttg gcgcggcttc gcatacaatc
     3421 ggtattctga tggattttac tgtgtagagc gaggattttt ccgtgccgcg aaagccgacc
     3481 aagaaggaaa ttgatggcga ttccatgctg attgaagaac gccgccagca cattctcgcg
     3541 ctggtgcaga gccagggccg cgttctggtc aacgaactgt ctgagagcct tggcatctcg
     3601 caaatcacta tccgtaaaga cctcgactat ctccaggcca agggactggt gcagcgcacc
     3661 cacggcggcg cgcttccgct ctctgccggt gccatgttcg acccttccct gcaggaaaaa
     3721 atgcgccagc acgcgcagga aaagcagcgc atcgccaatg ctgccgcaaa acttgttcag
     3781 gagggccagt gtgtcatgct tgactccggc accactacga ccgctgtcgc ccacgccttg
     3841 cgccggtttg cacacctcac cgtcattacc aacgccatta atatcgcagc cgaccttgcc
     3901 ggtacagatt tcgacgtcat tcttaccgga ggcactctgc gcaagaattc gttttctctg
     3961 gttgggccgc tggccgaaga cgtgctcgac gaaatgcatg ccgatgtgct ctttcttggc
     4021 gtggatggat ttgacctgga aatcggcctg accactccca acgttctgga atcgcgcgtc
     4081 aatcgagcca tggtcaaggc cgcccgtaaa gtcgttgtgg tctgcgattc aaccaaattc
     4141 caccgccgca gcctctcgcg catcattcct ccgtctgacg tccattgcgt catcacagac
     4201 cgcggccttt ctgaaacaga cgccgaggtc ctgcgcggcc tgggcatgga actggttctg
     4261 gtgtaataat ttcgatttca atcttttgtt gactttttat ttcgttttat gaacaaatgc
     4321 cagtaatact caatgtttga cgtcaccatc gcaggcgaaa tcaacctgga cctggtcctc
     4381 tatggtctac cgcgtaatat gcccacagaa cgcgagctgc tggcctctga tttccaggcg
     4441 actctgggca gctcctcggc cattctggcg cacaatctgg ctgttctcgg cgcaaatgtt
     4501 ggctttatca ctcgcgttgg aacggatgaa ctggggaaga tcgcgctgga acgcctgacc
     4561 gccagcggca cagacatgag ccgtacaacg ttcgacacaa agcattcgac tggaataacc
     4621 gtcctgcttc atcacggcaa ggagcggcac atcctcacct atccagggac gatgagagaa
     4681 atgacagtgc gcgatctgga cattgcatat ctcaaatcat cgcggcattt tcatctgtcc
     4741 tcactctatt tgcaaaccgg gcttgctccg gatctgcctg ggttatttcg tgaactgaag
     4801 cgtgctggcc tgaccatttc gctcgatacg aatgacgatc cggaagacac atggggcggt
     4861 gtgctggatg aactgctcgg gttgattgac cttctcctgc cgaatgaggc cgaagcgcgc
     4921 cgcatcgcaa aacggaacac gctggaagag tcgctcgccg tgctctcgca gcgcgttccc
     4981 tgtatcgccg tcaagcgagg cgcgcagggt gcgctcgtgc aggtaggaaa tacacggctc
     5041 acggcccccg ccgtacgtgt aaccccaata gatacagttg gcgcaggcga cagcttcaat
     5101 gcagggttcc ttttcgcatg gctgcgcaga ctcccgctcg acgcctgtgc ccgcgcgggc
     5161 aacatcaccg gagcactctc caccctgcgc ccaggcggaa ctgaggcctt ccgggaccgc
     5221 gaactgatga ttacattcct gaagcagcac aactttcctg ctgcggttgc ggagacaaag
     5281 gcataatgaa ctttgccccg gttgattgga ccatcgtcgt agcctacctc gcggcttcgc
     5341 tgctcatcgg cctgctgggc cgcaaatacg tcggcaacac cacccacttc ctcgttgccg
     5401 gacgcgagct gggtacctac atcggcatcg caacgctggc tgcaaccgaa atcggcacta
     5461 tcactttcat gtacaacagc gaactgggct accgctatgg gttcgctgca ttttccgccg
     5521 cgctcatctc cggcattgcc atgatttttg tgggccgcac cggattcgtc atccgtcgac
     5581 tgcgcgaact gcggctcatg acggtgccgg aatacttcga ggtaaagtac agtcgcggtc
     5641 tccgtatcgt caccggattt ctcgtagcac tcggcggcat cctcaatatg ggtgtcttcc
     5701 tcaaggtaga aggagaattt ctcaccgtcg tcagtggcct ggaccagaaa taccttgtcg
     5761 tcaccatgat cgccatcctg ctgctggagc tgatttacac aacagtcggc ggcatgatct
     5821 ccatcgtcat taccgactat ctgcaatacg tcctgctttc cgtggcgacc atcatcatca
     5881 ccatctacgc cgtgcacttt gccggatggc acgccatggt ggcaaaagtc acaactgtga
     5941 tgggcagcag cggattcaat cccatcaccg cgccgaaatt cggctggagc ttcctcatct
     6001 ggcagctgct gttgtggctt tccatccata cctgctggca gaccacagcc atgcgcatgt
     6061 tctcgaccag gagtccggag acatcaaagc gcgttatgac ctggacaggc ttcatttttc
     6121 tcggccgtgg aatgctgccg atgatctggg gcatcgccgc gctcactctg cttggtcatg
     6181 gagcactgac tggcggtgtt ccagggccaa tcgcgcatgg acactccctt gcacccattg
     6241 acgccatgcc cgcaatgctt gccatgctgc ttggaccggg aatccgcggc atcgttgtag
     6301 ccggaatgct tgctgctacc atgtccgtca acagctcgta tctgctcgga tggagcgcgg
     6361 tcatttctca ggacatcatt ggacccatgc gaaagcagat gggccgccca ccactggcag
     6421 acgcaacgca actgctcatc aatcgcatct gcaatttctg cgtcggattg tttctcatct
     6481 tctggggcct gctttacaca cccccgggag cggtctatct ctacctgaac atcaccggga
     6541 ccatctttct tgcaggggct tttatctgcg tcattggcgg actttactgg aagcgcgcca
     6601 acatcaccgg aggctactgc gccatggtcc ttggtgccgc cggcgccatc gttccatttt
     6661 tctttttgca taaaagcgaa agctttgccg gattcgccgc cttcggcctg tccgctgtag
     6721 gtttgctttt tggctctctc ttcggaaaaa gcacaaaagt cccgcaaccg cagatggcaa
     6781 ttccggaagg agaagcgcga tgaatgcatg gatctgcttc tgggcagtat ggctggtcat
     6841 ttcaggcgca gccttcgcta tcattacgct gattgtcgcc gtgaaaggat ttgaggacct
     6901 gcgcgccatg tttgcgggat tgagcggtga gaaaaagaaa tcatgagccg cgctctcatt
     6961 ctgaccgccc gagggaaaaa cctgcttttc tcagcaggga gcgtggcaga acaaaatcaa
     7021 ttcctccgct cagaatgaca tttgaatcgt atgcatagag tagaagtttt atgagcaatc
     7081 aggacaaatt cgttgccatg gtgacggcca gggcggaggt agacctcccg ctcagcaaat
     7141 tccagccgaa atccatggtg gttgcagaag aacacttgcc cgagcgcccg cgcttccctg
     7201 ccattgatta ccacaaccat ctcgacgcac tggagccgcg agatgttctg cgcgtcatgg
     7261 acgcatgcgg aatcgaaaaa gtcgtcaaca tcaccatgaa gacaggtgaa gccgcgctcg
     7321 cgatgattga caagttccat tccgcagcgc ccgatcgttt tgccaccatc ggctggatgg
     7381 attggtctga tgtgacacat cctgatttta ttgctcgttc gctcgaacgg ctggaaaggc
     7441 tcgccgaacg cggcgcaaag ggcattaagt tctggaaaga cctcggcctg agcgtgcgcg
     7501 acgcgagcgg ccatctgctc cgcgtagacg acgagcgcct cgcgccactc ttcgataaag
     7561 ctgcggaact gggcattccc gtcatgtttc acatcgccga cccggcggct ttcttcctgc
     7621 ccatagatga gcacaacgaa cgttatgaag agctggccgc gcaccctgac tggggatttt
     7681 atggatcgca atattccaag tgggaattac tcgaccagcg cgatcgcgtc attgcacgcc
     7741 atccaaaaac aacgttcgtc tgcgcccatg ttgctgaatg cggagagaat ctcgcgcgcg
     7801 ttacaaagct gcttgagagc tgcccccatg tctacattga catcagcgcc cgcgcatcgg
     7861 aattaggccg ccagccctat tctgcccgcg aactcttcct gcgcttttcc gaccgcatcc
     7921 tcttcgggac agaccttctg ccggaagaaa cgatgtaccg cctctatttc cgttttcttg
     7981 aaaccgccga cgaatacttt gagtatcctt ctcacgcttc acgccagggc cgctggaaca
     8041 tctacggact gtatctgcca gatgacgtgc tgcgaagggt atatcgggaa aatgccctgc
     8101 ggcttttgaa caccgcctga ttcatgcttt gggacgaaat actttagctg ccattccgga
     8161 aacgaatctg cgtggcagca atcgctcgat cagcaccatc gcctttcctg ccctgtaggg
     8221 aatgacccag tgctctccgc gcgcaagccc ctcaaggccg atgcgcgcca cttcttcagc
     8281 agattgccgt ccgcgcccga actggttgcg cgttcctgca atttcgtgaa attccgattc
     8341 cgtcggcccg ggacacagtg cgctcacttt gattccgtgc tctgccactt catgcgccag
     8401 ggcctcggca agaaagcggt caaagacctt cgtcgcagca tacgtactca tgtacgggac
     8461 cggctggtat gcggccgtgg acgcgacaat catcacatac cctcgcctcc gctcgatcat
     8521 cttcggcaga aacaggcgcg tcaaatgcac caccgcagca cagttgactt caaccatgcg
     8581 acgctgccag gctgcatctc cctcggcaaa ccggccgtag tggccaaacc ctgcattatt
     8641 cacgagaacg tctacaacca gccccgctcc ttccgtagca gcaaaaagtg catccggagc
     8701 ggacggatgg gcaatgtcag cagccaccac acaggtctga ataccgtact ggccttcaaa
     8761 acgcgatgct gtcgcttcga gccgctcttt tcggcgggcc gtcaggacga ggtttgtccc
     8821 cgccgcggcc agttgttcgg ccaatgcaag tccaatgccc gcgctggccc ctgttaccaa
     8881 tgcccatcgg ccctggaatg cttcacgaag caatttggga ctcttcatga tttatctttc
     8941 cggtaatctc aggatatgtt ggctcgtcct ggtatcttat tgcgcgcgga ggccttgctg
     9001 gttctcgttg ccagctgtgc cggcttctcc atgcttcacg gcagttggct tatctttgct
     9061 ctgctctttc ttgcgcccga catctcgctc ctcggctata tggcgcgaaa tagtctgcgc
     9121 atcggcacga cgctctacaa cgccgcacat tcttacatcg gccctgcggt cctgggactg
     9181 ttatcatggc gcctgcattc gcctcgcttc ggacagcttg ccgccatctg gatagcgcac
     9241 atcgccttcg accgcctgct gggctatggg ctgaagttcc ccaatgcatt caagcctacg
     9301 cacattcaga gcgcggctat ttttgctgga actcctcgcc aatccactgg gtaaacgcat
     9361 ccaggctttg ttcccgtccc agaaactgtc gcacaatctc ttttcccgga agcgatccac
     9421 ccggctcaag cactgccttg cggtagcgca ttgccaccgg gtcatccagc aggtcctcgc
     9481 ggttgaactg gctgaagaaa tcgagcgcaa tcaccttgtc gtacaggtac gtgtagtagt
     9541 tcgatgagta cccggtcaga tgcgtaaagc tcgcatacat gcgattgccg tcaatccacg
     9601 tatacggcag gaatttcgtg tagtcggcct gcaacaaaac atccaggttc aacgtcgccg
     9661 ggtcttcgta gtgtgtatcc agcgaatagg tcgtataaaa gagctgcgtc cgtacccagt
     9721 ccgctcgtcc aaacgccccg gcccggttca tcttttctac cagttctgcg ggaatcggct
     9781 cgtccgtctg gtaatgtttc gcaaaggtgg ccagcagctt cgcatcccgg aagaactctt
     9841 ccagcatctg cgaaggcact tctacaaaat catcttccgt cgcgatgccg ctgatgcccg
     9901 cccacttctg ctttccgccc agaagcgcgt gcatcaggtg gccaaattca tggaagaacg
     9961 tcaccacatc ggagtactgc aacagcgccg ggtttccgtc cttcggctcc ggaaaattgc
    10021 agaccagagc ggcctccggg atctgcctgc ccgcaatgcc gggaatcagc ggatgcgcgc
    10081 tgaaccactt gtccttacct tcgcgtgggt gcatgtcgag ataaaaccgc ccgatgcgct
    10141 tcccgccatc aaaaacgtcc catgcgccca ctgactcatg ccatacctca gcatcttctg
    10201 ccggccggaa gctgacgcga aacagcttct gcgccgtctc cagaacgccc ctttccaccc
    10261 gctcataggg aaaatagggc cggacagtct gcgaatcgaa atcgtatgcc gagcggcggt
    10321 attgttcata ccagtaggag cggctgtccg catcaatggc cacaattttt ggctcgtact
    10381 gccgggcaaa ctcgagcacc gcggcatatt cacgctccgc tccggccttc gtggcctcat
    10441 ccagcccgtc cagaaacgcc cgcagctcct tcgacgaacc catcatctgg tcagcagtgg
    10501 ccagatccgc ccacgtttca aaacccagca gattggcaat ctcgcgccgg gtcttcagca
    10561 agtcgagcag aacgccttca ttcgccggat acgcccgggt gttgtaagcc agaaacatct
    10621 gcaggcggag ctttgggttg cgggcaaagg tcatcactgg gaggtagtca gggaaatcgg
    10681 tggtcagggt aatctggccg tgttcgtcag ccggatggtt cgctggaaaa tcctcaggca
    10741 gcccctccag ttctgccttg tcctcgacca cgaccttgtt gacgttctcc tgcacattgc
    10801 gccggaaaac cagcgagaga tgcgtggcct tgtcatgcaa ttccctgagc cgggcgcggg
    10861 tcttctcgtc cttgtccaca ccagcgagcc ggtattgcaa cagcgtccgc tcaacgtaat
    10921 ggcgtgtgcc gctgtctgct ccggagaggt ccatcgcttc cagggcgcga tagacttcct
    10981 ggttcagagc gagcagggta cccgcttcag ccaccttctg gatcagggtc tgggccgcgt
    11041 cccgcacctt cttttccgga tgcacgctgt tcagcagcgc tgtctgcgag cgggccatgc
    11101 tcagctcagc cacggcgctg tcataagcag aaagcgtatt ttctaccgtt cggagaccgg
    11161 aaacggccag cagttgctcc aatgcttcct tatgccgttc cagagcagca ttgacccagg
    11221 attccacacc ggtttcgtct gccgcccctt cccatggacg tgtaactttt gttgcaacaa
    11281 cagatacgga ttcagccaca aaaatttccc ctaaacctta gattcaggtt agcctagctt
    11341 ttgacttcgc gcacggcgcg aaagagtact ccaccggcgg atatggtgtt agaatgcgcc
    11401 gaaggcgctt ttatccgtaa gttgcaagta tttacatgaa tcagactgtt ccagcaattt
    11461 cgccacaatt tatccggtca cgcattggca aggtgtgcgt tgccatcatt ggctccactc
    11521 ccgccgaaat gcttgaaaaa gcacaggaaa ccatccgcga gaacaccttt ctcgagttcc
    11581 ggctggatta cctcgataaa cccctggccg ctgtcccaaa actgaaacag tttctggcag
    11641 agcggcccga cgtgaccgcc attgccacct gccgccgtgt tgctgccgga ggcaagttca
    11701 agggcaaaat tgccgacgaa ctggacatcc tgagcaaggc cgctgcggca ggcttccatc
    11761 ttgtggatgt cgagcttcag accgcagaaa acgtcaaaga ggcagaactc gagaaactgc
    11821 gcgcccgcgg cgccgccctc atcatctcct ggcatgattt tgccgctacc aaagatctgg
    11881 acggcatctt cgagcgcatc cggccctatg agccggactt catcaagatc gtctcgacgg
    11941 ccaaaaacct ttccgacaat gtcaccatga tgcacttcct tgagcgcacg cgcgacatgg
    12001 ccaatatgat tggcatttgc atgggcgacc agggaatcat cagccgcgtc cttggcattc
    12061 gcgcaggcag cgtctttacc tttgccgctg ccactccggg agaagaaacc ggtccaggcc
    12121 agatcgccgc ccgcactctg cgcgaaacct accggattga ccagattgac gccgccacca
    12181 aagtttatgg agtggccgga aaccccatca gccattctct ctccccgctc atgctgaaca
    12241 ccgcctttcg ccgcgagacg gtcaatggag tattccttgc cctgcagacg gcgaagatca
    12301 aagaccttct gaccctggtg cgcgaggtgc ccatccacgg actggccgtg accatgccgc
    12361 tgaaagaaga gattctggag cacctggaaa agaccgaccc ggtttcggaa aaaatcggtg
    12421 cctgcaacac cgtcgttcgc gcgcaggacg gcaaactata tggctttaat accgatgtcg
    12481 ctgctgtcgt gcgcccgctg gagcgtcgcc tgcagcttag aggcgcaaaa gtccttgtcc
    12541 tcggcgctgg cggagcggcc cgcgccgccg tcttcggcct gaaggaaaag ggtgcggaag
    12601 tcttcatcct gaaccgcacc gccgccgacg gacagaagct ggcccgccag gcaaaggccc
    12661 gtaccttccg ccgtgaccag ctcgccaaaa cgcagttcga catcatcatc aatgccactc
    12721 ctgtgggaat gcagggcgtc aagccgaacc acatccttga gccaaaggaa atcaacgccc
    12781 gcctggtctt cgatatggtc tacaacccaa tcgaaacacc gctgctgcgc atggcccgtg
    12841 aaaaaggcat tcctgtcgtt acgggagtgg aaatgttcgt ccaccagggc gcgcgtcagt
    12901 ttgaaatctg gaccggcaaa cccgccccgg aagaagaaat gtt
//
LOCUS       DASRBV010000229        15312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_575154, whole genome shotgun sequence.
ACCESSION   DASRBV010000229 DASRBV010000000
VERSION     DASRBV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15312
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575154"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..544
                     /gene="rplF"
                     /locus_tag="VFA02_08580"
     CDS             5..544
                     /gene="rplF"
                     /locus_tag="VFA02_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZU09948.1"
                     /translation="MSRIGKKPIPLPQGVKYSVQGNTVVVEGPKGKLEQRIPEGIRLV
                     TQDGHIVAERENDSQAAVHGLTRALVANAVEGVTRGWSKDLDIVGIGYRVELKGKDTV
                     VFTLGYSHPIEFPLPTGITATIDPKQTRITISGIDRQKVGQVAADMRALRKPDPYKNK
                     GVRYADEKLKKKAGKAGAK"
     gene            668..1024
                     /gene="rplR"
                     /locus_tag="VFA02_08585"
     CDS             668..1024
                     /gene="rplR"
                     /locus_tag="VFA02_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZU09949.1"
                     /translation="MIAQKNRDEIRRRIHARIREKVAGSPERPRLSVYRSLNHIYAQV
                     IDDTKGVTVASASSKAAKLKTGGNVAAAKEIGKLVAERAKEKGITKVVFDRGGYLYHG
                     RIKALADAAREAGLEF"
     gene            1115..1624
                     /gene="rpsE"
                     /locus_tag="VFA02_08590"
     CDS             1115..1624
                     /gene="rpsE"
                     /locus_tag="VFA02_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZU09950.1"
                     /translation="MATLRKKLDAGQYNLKDQVVAINRVTKVVKGGKNMSFAALVVVG
                     DPASGVVGYGSGKAKEVPQAIRKGIESAKKNLIRVNLTETTIPHQVLGRFGSGQVLLK
                     PAPEGTGVIAGGAVRAVMTSAGVQNVLTKSLGTANPHNVIKATFDALAQLRDRQEVAA
                     VRGKQVEEL"
     gene            1627..1815
                     /gene="rpmD"
                     /locus_tag="VFA02_08595"
     CDS             1627..1815
                     /gene="rpmD"
                     /locus_tag="VFA02_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZU09951.1"
                     /translation="MAETKAKIKIQYYRSSIQAPVKHKKVVKGLGFTRLNQIVEREDT
                     PSIRGMVKTVPHLVRIVE"
     gene            1943..2383
                     /gene="rplO"
                     /locus_tag="VFA02_08600"
     CDS             1943..2383
                     /gene="rplO"
                     /locus_tag="VFA02_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZU09952.1"
                     /translation="MNLSNLRAPKKANRNKKRVGRGMGSGHGKTSTRGHKGQGSRSGS
                     SLMRGFEGGQMPLHRRLPKRGFTNIFRVEYNTLNLDRLIELGETELTLEYFEKKGLIK
                     KKNGLLKILGNGELTKPITVHAHKFSKSAQEKIEKAGGKAILVA"
     gene            2425..3822
                     /gene="secY"
                     /locus_tag="VFA02_08605"
     CDS             2425..3822
                     /gene="secY"
                     /locus_tag="VFA02_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZU09953.1"
                     /translation="MLEKFANIFRIPDLRKRVLFTMAMLAVYRLGSFIPTPGVNTTAL
                     EQFFDLQGGSALGLVNLFSGGNLRRLTVFALGIMPYITASIIFQLLTVVYEPLARLQK
                     EGELGRRKITQWTRYMTVGLGIVQSLAIALTLSHGAGGLQLVLNPGPGFVFMTVITLT
                     TGTTFIMWLGEQITDRGIGNGMSLLIFAGIVVGLPRAIQELVETARSEKFGPFTVPAL
                     ILLVAAMVAVVVFIVFVERSERRIPVQYAKRIVGRRMMGGQSTHLPLRVNSGGVMPII
                     FAASVLSAPLLFAQSSFVRNSAALTRIFDALHAGEPWYEFLYMVAIIFFAYFYISIVF
                     RPDDIADNMRKYGGFIPGIRPGKRTSDFINDILTRITLVGAIYLIVVAIIPQILISGI
                     HLNHLWLVGPLFEHMPLWVTNGLGFNFYFGGTSLLIVVGVAMDTVNQVESQLIMRHYE
                     GFTPRSGRIRGRRAW"
     gene            3823..4548
                     /locus_tag="VFA02_08610"
     CDS             3823..4548
                     /locus_tag="VFA02_08610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZU09954.1"
                     /translation="MAIAVDERQTGGRKTVKAVPGPVLLLGAPGVGKGTQAKAIMAAW
                     GIPQISTGDILRANVSGDTELGKKARAVMDRGELVPDDLVNEMVADRLKQPDTANGYI
                     LDGFPRTLGQAEWLDAHLTKQSGGLPVVAVSIKVSYTQLLRRITGRRNCPVCKSIYNI
                     YLQPPKVDELCDLDGTPLARRSDDTEAVFEERMRTYESQTAPVVEHYRALGRFEEVDG
                     EQPVEAVTAEVMAVVLRLRGTAG"
     gene            4548..5300
                     /gene="map"
                     /locus_tag="VFA02_08615"
     CDS             4548..5300
                     /gene="map"
                     /locus_tag="VFA02_08615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZU09955.1"
                     /translation="MAIVIKTPQELEKMRRSGVVVREVLEYVRGFVRPGATTMDLENA
                     AARKMEELGVKPAFKGYHGYPCVLCTSINSEVVHGIPSTKRVLREGDIVSIDTGVIID
                     GYYGDSAITVPVGAKIAPKAQRLLDVTKASLESGIQAVKPGATLGDIGAAVQDVVEAE
                     GFSVVREFVGHGIGTSLHEDPQVPNFGRRGQGMKLREGMVLCIEPMVNSGKPDVQVLA
                     DGWTAVTQDGSLSAHFEHTVAITKDGAVVLTA"
     gene            5350..5568
                     /gene="infA"
                     /locus_tag="VFA02_08620"
     CDS             5350..5568
                     /gene="infA"
                     /locus_tag="VFA02_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZU09956.1"
                     /translation="MSKEDAIEVMATVLETLPNAMFKVELENKHQVLAHVSGRMRKNF
                     IRILPGDRVAVELSPYDLTRGRIVYRYK"
     gene            5696..5809
                     /gene="rpmJ"
                     /locus_tag="VFA02_08625"
     CDS             5696..5809
                     /gene="rpmJ"
                     /locus_tag="VFA02_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZU09957.1"
                     /translation="MKVRASVKKICDKCKIIHRRGVVRVICENSKHKQRQG"
     gene            6036..6422
                     /gene="rpsM"
                     /locus_tag="VFA02_08630"
     CDS             6036..6422
                     /gene="rpsM"
                     /locus_tag="VFA02_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZU09958.1"
                     /translation="MARIAGVDLPRQKQARIALTYIYGIGNPRALSILEKANVDPFKK
                     IQDLGEDEVNRIRQVIEDEGAVEGDLRKDVSMHIKRLIDIQSYRGLRHRRSLPVRGQR
                     THTNARTRKGPRKGTIANKKKATAKT"
     gene            6422..6838
                     /gene="rpsK"
                     /locus_tag="VFA02_08635"
     CDS             6422..6838
                     /gene="rpsK"
                     /locus_tag="VFA02_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZU09959.1"
                     /translation="MAKAQQGAGKAARNKKFKKRERKNVPYGVVHIQASFNNTIVTIT
                     DQQGNTLSWKSSGSLGFRGSRKGTPFAAQQAAINAANQAREHGLRSVDVRVAGPGSGR
                     ESAIRALAAAGIEVRSIRDVTPIPHNGCRPPKRRRV"
     gene            7147..7776
                     /gene="rpsD"
                     /locus_tag="VFA02_08640"
     CDS             7147..7776
                     /gene="rpsD"
                     /locus_tag="VFA02_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZU09960.1"
                     /translation="MARYTGAVCRLCRREGVKLFLKGAKCFSDKCPIEKRNFAPGQHG
                     RDRKAKVVGYGLQLREKQKAKRIYFTLEGQFREYYEKASRAQGVTGELLLQQLERRLD
                     NVAFRLGFASSRRQARQIVRHGHVEVNGRKVNIPSFQVSVGDEIKVRPNSQKLSVVET
                     AREFASHQPAPAWLAVDHANLSGKVLALPKREDIQLPVNEQLIVELYSK"
     gene            7908..8999
                     /locus_tag="VFA02_08645"
     CDS             7908..8999
                     /locus_tag="VFA02_08645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZU09961.1"
                     /translation="MLWRGFQKPKRLAVDTDTLTNTYGKFSAQPFERGFGTTIGNALR
                     RTLLSSIEGAAVTAVKIEGVLHEFQSITGVVEDATDIILNLKQIPFKLNGEGPKALYV
                     RAEQPGVVTSGMIEADGDVEILDKDIYIATVSEGGKLDMEMRLKRGRGYVSADKNFDS
                     DLGIGFIPVDSVHSPVRKVNFTVDAARLGQITDYDKLTLEVWTNGSVLPADAIGLAAK
                     LLKDHMSIFINFEEEIEAEQQAEERVQLRNDNLNRSVEELELSVRSYNCLKNANIQTI
                     GELVQKTEAEMLKTKNFGRKSLNEIKEILAQMGLSLGMKIDEHGNAVPGPTSVLPSAQ
                     LAASRYNQFDEEEGLDEADIPLQSEPENF"
     gene            9090..9566
                     /gene="rplQ"
                     /locus_tag="VFA02_08650"
     CDS             9090..9566
                     /gene="rplQ"
                     /locus_tag="VFA02_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZU09962.1"
                     /translation="MRHRNAGYKLGRNTSHRRALLRNLATSVILEDRVETTVAKAKAV
                     RPHVERLITLGKKGDLHARRQALAYLQTRDAVTRLFDTVAPRYGDRNGGYLRIVRRGF
                     QRGDGAEKAFIELLGAEAVLDEKRQKRAEARQKRREELQKAMEAEQQQANEGGEQA"
     gene            complement(10033..10656)
                     /locus_tag="VFA02_08655"
     CDS             complement(10033..10656)
                     /locus_tag="VFA02_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09963.1"
                     /translation="MYKECRHIFTGGNKCGSPALKGDDFCYYHSNTRKRRKPGSESYN
                     AIAREDYPLNLPVLDDPEAIQLAISDVVIALAAGVLDQRRAHSLLYGLQIASQHFRAL
                     SAITRAKTVSAPTTPATRESAQASAPQSVREVFFDSTGEPLGPEKECPDPEDLPADRD
                     QITHPSMLLMDDIQSGHLDPADVDTRHPDMDGLQTLALCSPTCPARD"
     gene            11026..13584
                     /locus_tag="VFA02_08660"
     CDS             11026..13584
                     /locus_tag="VFA02_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09964.1"
                     /translation="MKLHRFCLLIAFLLVVASLTAAADTAPFDLAGPKVDVRITRSGK
                     TLPIGKVPNLQAGDRLWIHAQMPAGQSVKYLMVVVFLRGSTNPPPDKWFYKAETWDKK
                     VAQEGIFVTVPDGAQQAVVLFAPSTGGDFNTIRDAVHSRPGSFVRASQDLNQLSLDRA
                     RLDAYVEAVRQINETNPDRLKDVSPLLARSLSIKLDQDCLQKVVEQQASCLVQKQDQL
                     VMDDGHSLSVTDALTSGASSDLAMQLSYTQRAGAGYYSPYIASVMDMAKILEGLHTAQ
                     YQYIPALNIVKNDEVELKLNTPPSFRNPKSVIVMALPAIDKGHLPPLRPVDEKQVQCI
                     AKDNPVLPAEGAPLDFATDYAHDMFLRVKGKNGKDLDIPVKAEPAHGGFVADTHGISM
                     ENIGSDTGTLHGWWGFEQYSGPVFHFQTGRSAKWELAATDKHALVVGREDTLHLTSGD
                     ATCVDSVTAKDAAGKEIAVTWKADQPDQLEIKVPLKDATPGPLVLAVKQAGAKQPQEM
                     HLEGLEEAGKFESFTINAGDTQGVLAGNRLDEVASLNLKGVIFKPGKLANTENGDALT
                     LEAADSKSASALQAGTKTTAQVFLKDGRALDVPAAVVAPRPKVQLLSKSVQPAESVKA
                     THIQLTDTDLVPLDSKLAFSVKAITPATFSPDEKIEVATEDGSFHTTLSMADGGITLQ
                     DAKTAVVSLDPAKSFGASAFGPLRFRPVSGNGTTGDWQPLATLVRLPMLDALQCSGGP
                     GQTCTLKGSNLFLLQAVAADAQFAQGMQVPDGFAGNALQVPHPVNGTLYVKLRDDPNA
                     VSTAVLPRPAPQRAALVAAPATSAQPAPTPQQQAQPEPEPAAAAPQPQANAPHE"
     gene            14084..>15312
                     /locus_tag="VFA02_08665"
     CDS             14084..>15312
                     /locus_tag="VFA02_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZU09965.1"
                     /translation="MAEATKKIASSVIASTASQPASALKFERVFSKAGVSPYDEVQWE
                     RRTASITDASGKTIFEQKDVEVPVDWSMTATNIVASKYLHGQLETPERETGVRQLISR
                     VAETIRDWGLAGGYFSAEDAAVFHDELVHLLVTQKAAFNSPVWFNVGCDRLEPNSDAQ
                     NWHWNPHTCAVEFSVTGYRKPQCSACFINAVDDSLDSILNLAKTEGMLFKWGSGTGTN
                     LSPIRGSMELLSGGGTASGPLSFMRGFDAFAGVIKSGGKTRRAAKMVILNVDHPDIMD
                     FIECKMKEEAKAWALMREGYDGSGPDSEAYSSIFFQNANNSVRVTDEFMRAVEKDGEF
                     TTYTVKDHKPVKTYKAREIMQKIADATWHCGDPGMQYDTTINKWHTSKNTGRINASNP
                     CSEYMFLDNSACNLASF"
BASE COUNT         3389 a         3958 c         4827 g         3138 t
ORIGIN      
        1 agaaatgtca cgtattggaa agaagccgat tccgttgccg cagggtgtga agtattccgt
       61 gcagggcaac acggtggtgg tggaaggccc taagggcaag ctcgaacagc ggatacctga
      121 aggcatcagg ctggtgaccc aggatggtca catcgttgca gagcgtgaga acgattcaca
      181 ggccgctgtt catggcctga cccgcgctct ggttgccaat gcagtggaag gcgtgaccag
      241 gggctggtcg aaggacctcg acattgtcgg tattggttat cgcgtggaac tgaagggcaa
      301 ggacacggtg gtcttcacgc tgggctattc gcacccgatt gagttcccac tgcccaccgg
      361 catcaccgcg acaattgatc cgaaacagac ccgcatcacc atcagcggca ttgaccgcca
      421 gaaggtgggc caggtggccg ccgatatgcg cgcgctgcgc aagcctgatc cgtacaagaa
      481 caagggcgtg cgctacgcag atgagaagct gaagaagaag gccggaaagg ccggagcgaa
      541 gtaagtagtt cgttgctggt tgttcgtctc cggtagtttt gaaccaacaa cgaatgactg
      601 gcaactaaga actgttatcc cgaacgtggc cgcagggtgg tcacgctgtt aggaaggacg
      661 aaagacaatg attgcacaaa agaacagaga cgagattcgc cggcgcattc acgcccgcat
      721 ccgcgaaaag gttgcaggga gcccggagcg cccgcgtctg agcgtctacc gctcgctcaa
      781 ccacatttat gctcaggtga ttgatgatac gaagggcgtg actgtggcct cagcgtccag
      841 caaggcggcg aagctgaaga cgggcggcaa tgtggcggcg gcgaaggaaa tcggcaagct
      901 ggtggccgaa cgcgcaaaag agaagggcat tacgaaggtc gtgtttgacc gcggcgggta
      961 tctctatcat gggcgcatca aggcgctggc tgatgccgcg cgtgaagccg ggctggagtt
     1021 ttaagcctgt tattttttaa ggggcacggc ctgggccgcg cctgaaaagg aagataaatc
     1081 cggggcttca gcccctgagg aaatcaggaa gcgaatggca acactgagaa agaaactgga
     1141 tgccgggcag tacaacctga aggaccaggt ggtcgccatt aaccgtgtga cgaaggtggt
     1201 caaaggcggt aagaacatgt cgtttgcggc gctggtcgtg gtaggggacc ctgcttcggg
     1261 cgtggtcgga tatggctcgg gcaaggcaaa ggaagtgccg caggcaatcc gcaaaggcat
     1321 tgaatcggcg aagaagaacc tgattcgcgt gaaccttacc gagaccacga ttccgcacca
     1381 ggtcctgggc cgctttggtt ccggacaggt gctgctcaag ccggcgccgg aaggtactgg
     1441 cgtgattgct ggcggagcgg ttcgcgcggt catgacttcg gctggtgtgc agaacgttct
     1501 gaccaagagc ctgggcacgg ccaatccgca caacgtgatt aaagccacgt tcgatgctct
     1561 ggcacaactg cgcgaccgtc aggaagtggc agcggtccgc ggcaagcagg ttgaggagtt
     1621 atagtcatgg cagagaccaa ggcaaaaatc aaaatccagt actatcgctc gtccattcag
     1681 gcgccagtga agcataaaaa ggtcgtcaag ggccttggct ttacccgcct gaaccagatt
     1741 gtggagcgcg aggacacacc ttccatccgt ggaatggtca agacggtgcc gcatctggtg
     1801 cgtatcgtcg agtaagttgc tggctgttag ttgccagtcc ccaataaagg cggcaagtta
     1861 ctgataacta agaactaaca acttggaact aagcaaatgc gagtcggtca gggcaagcct
     1921 ggctggcgta gagcgaggac atatgaatct ctccaatctc agggcgccga agaaggcgaa
     1981 caggaacaag aagcgcgtag gccgtggtat gggttccgga cacggcaaga cctcgacccg
     2041 tggacataag ggacaaggct cccgctcggg ctcgagcctg atgcgcggtt ttgaaggcgg
     2101 ccagatgccg ctgcaccgcc gtctgcccaa gcgcggcttt accaatattt tccgtgttga
     2161 atacaacacg ctgaacctgg accgtctgat tgagctgggc gagaccgagc tgacgctgga
     2221 atattttgag aagaagggcc tgatcaagaa gaagaatggc ctgctcaaga tcctgggcaa
     2281 tggcgagctg acgaagccca tcaccgtcca cgcgcacaag ttctcgaaat ccgcacagga
     2341 aaagatcgag aaggcgggcg gcaaggccat tttggtagcc taagaagctg agggccggaa
     2401 ccggccctcc ggatgtctgt tttcatgctt gaaaaattcg ccaatatctt ccgcatccct
     2461 gacctgcgca agcgagtgct gttcactatg gcgatgctcg cggtctaccg cctcggttcc
     2521 ttcattccaa caccgggtgt gaacaccaca gcgcttgagc agttttttga tctgcaaggg
     2581 ggaagcgccc tcggcctagt caacctgttc agcggcggca acctgcgccg tctgacggtc
     2641 tttgcgctgg gcatcatgcc gtacatcacg gcctcaatta ttttccagtt gctgaccgtg
     2701 gtttatgagc cgctggcccg ccttcagaaa gagggcgaac tgggccgccg gaaaattacg
     2761 cagtggaccc ggtacatgac cgtaggactg ggcattgtcc agtcgctggc gattgcactg
     2821 acgctgtcgc atggagcggg cgggctgcaa ctggtgctga atcccggccc cggatttgtt
     2881 ttcatgaccg tcatcactct gaccacaggc accaccttca ttatgtggct gggtgagcaa
     2941 attactgacc gcggcatcgg caatggcatg agtttgttga tctttgccgg aatcgtcgtc
     3001 ggcctgcccc gcgccattca ggagctggtg gagacggccc gttcggaaaa gttcggaccg
     3061 tttactgttc cggcgttgat tctgctggtt gcggcgatgg tcgccgtggt tgtcttcatc
     3121 gtatttgtgg agcgcagcga gcgccgaatt ccggtgcagt atgccaagcg cattgttggg
     3181 cgcagaatga tgggcgggca gtccacccac ctgccgctgc gcgtcaactc cggcggtgtg
     3241 atgccgatca tcttcgccgc ttccgtgctt tcggcaccgt tgctctttgc gcaatcatcg
     3301 tttgtgcgca acagcgctgc tcttacgcgc atttttgacg cgctccatgc aggggagccg
     3361 tggtacgagt tcctgtacat ggttgccatc attttcttcg catatttcta tatttcgatt
     3421 gtcttccgtc cggacgacat tgcagacaat atgcgcaagt atggcggctt tattcctggc
     3481 atccgtcccg gcaagcgcac ctcagatttt attaacgata tcctgacgcg cattacactg
     3541 gttggcgcga tttatctgat tgtggtggcg atcattccgc agattctcat cagcggtatc
     3601 cacctgaacc acctctggct agtggggccg ctctttgagc acatgccgct ctgggttacc
     3661 aacggccttg ggttcaactt ctacttcgga ggaacgtcgc ttctgatcgt tgtcggtgtg
     3721 gccatggaca cggtcaatca ggtggaatcg cagctgatca tgcgccatta cgagggattt
     3781 acgccccgca gcggacgcat ccgtggaagg cgggcctggt aagtggcgat agctgtggat
     3841 gaaaggcaga cgggcggcag gaagacggtg aaggccgttc ctggcccggt ccttcttctc
     3901 ggcgcaccgg gcgtaggcaa aggcacgcag gcgaaagcca ttatggcggc ctggggcatc
     3961 ccccagattt ctaccggaga catccttcgc gccaatgttt ccggcgatac ggagctgggc
     4021 aaaaaggcca gggccgtcat ggaccggggc gagctggtgc cggatgatct ggtcaacgag
     4081 atggtggcag accgtctgaa gcagcctgat acggcaaatg gttatattct ggatggtttc
     4141 ccgcggacac ttgggcaggc agagtggctg gacgctcacc tgacgaagca gtctggaggg
     4201 cttccggtgg ttgctgtcag cataaaagta agctatactc agttattgcg ccgaatcacc
     4261 ggaaggcgca actgtccagt ctgcaagagc atttataaca tctacttgca gccgccgaag
     4321 gtggatgagt tgtgcgacct cgacggcacg ccgctggcgc ggcgctctga tgacaccgaa
     4381 gcagtttttg aggagcggat gcggacatac gagtcgcaga ccgccccggt cgtggagcac
     4441 tatcgcgctt tggggcggtt cgaggaagtg gacggcgagc agccggtgga agcagtcacg
     4501 gctgaggtca tggctgttgt tctgcgtttg agagggacag ccggctgatg gccatcgtaa
     4561 tcaagacgcc gcaggaattg gaaaaaatgc ggcgcagcgg ggtagtcgtt cgagaggtcc
     4621 tggaatacgt gcgcggtttc gtgaggccgg gcgccacgac gatggacctt gagaatgcgg
     4681 ccgcgcgaaa aatggaagag ctgggcgtga agcctgcatt taagggatac catgggtatc
     4741 cctgtgtgct gtgcacctca atcaacagcg aagtcgtgca tgggattccg tccacgaagc
     4801 gggtcctgcg cgaaggcgac atcgtttcca ttgatacggg cgtgattata gatggctact
     4861 atggtgattc ggccatcacg gtcccggtgg gtgcgaagat tgctcccaaa gcgcaacgcc
     4921 ttctggatgt aacgaaggct tcgcttgaga gcgggatcca agcggtcaag cctggagcta
     4981 cgttgggcga cattggcgca gctgtgcagg atgtagtcga agctgagggt ttcagtgtag
     5041 tccgcgagtt tgtggggcat gggattggga cttcgctgca tgaagatccg caggtcccga
     5101 actttggccg ccgtgggcaa ggcatgaagc tgcgggaagg catggtgctc tgcattgagc
     5161 cgatggtgaa ttcaggcaag ccggatgtgc aggtgctcgc cgatggctgg acggcagtga
     5221 cacaagacgg cagtctgagc gcgcattttg agcacacggt ggccataacg aaggacggcg
     5281 ctgtggtgct gacggcataa gaatttttca gaggggccgg cgaaggccgg cagaagcggg
     5341 aattagcgat tgtcgaagga agacgcgatt gaggtaatgg caactgttct ggagacgttg
     5401 ccgaatgcga tgttcaaggt cgagcttgag aacaagcacc aggtgcttgc tcatgtctca
     5461 ggccgcatga ggaaaaattt tatccgcatt cttcccggag accgggtcgc ggtggagtta
     5521 agcccctacg atctgacacg aggaaggatt gtttaccggt ataagtagct tttagccgtt
     5581 agcttctagc tcttagctat agcagcggcg ggaacaacaa tcatgctttg cgcgaagata
     5641 gcagggcaag gaacaagcta gaggccaaca gctaatagcc aggagctgta gttcaatgaa
     5701 ggttcgtgca tcagtaaaaa agatttgtga caagtgcaag atcattcacc gccgtggcgt
     5761 ggtgcgcgtg atttgtgaga actcaaagca caagcagcgc cagggctgaa gtagttctta
     5821 gttgtcagta gttggtctcc agtgctggag actgacaact ggggactaga gactagttac
     5881 tttcaacagg tctggatctg caggtgttgg ggctagttag ccaggaaagg cttgcgatga
     5941 accccatgat ctgaaaggcc tgttttaact gcttccctcg aagagggtgg cacaactgtt
     6001 ccggatttgt tctggaactg ctaaagaagg gaaccatggc acgtattgct ggcgttgatc
     6061 tgccccgcca aaagcaggcc cggatcgcgc ttacttatat ctacggcatc gggaaccctc
     6121 gtgcgttgag cattctggag aaggcgaacg ttgatccgtt caagaagatc caggacctcg
     6181 gcgaagacga ggtaaaccgc atccgccagg tcatcgaaga tgaaggcgcg gtcgaaggcg
     6241 acctgcgtaa agacgtctcg atgcacatta agcggttgat tgacattcag tcctatcgtg
     6301 gactgcggca ccgccgcagc ctcccggtgc gtggacagcg cacccacacg aatgcacgca
     6361 cccgcaaagg gccgcgtaag ggcacgatag cgaacaagaa gaaagcgacg gcgaagacct
     6421 aatggcaaaa gcacagcagg gcgccggaaa ggccgccagg aacaagaagt tcaagaagcg
     6481 cgaacgcaag aatgtgcctt atggcgtggt gcacattcag gcttcgttca acaacaccat
     6541 cgttaccatt accgaccagc aaggcaatac gctttcgtgg aaaagctcgg gttcgctcgg
     6601 attccgcggg tcgcgtaagg ggacgccgtt tgctgcgcag caggccgcca tcaacgccgc
     6661 caatcaggcg cgtgagcatg ggctgcgttc ggtggatgtc cgtgtggccg gtcccggttc
     6721 gggccgcgag tcggcgatcc gcgcgctggc cgcagcagga atcgaagtgc gttccattcg
     6781 tgacgtcacc ccgattccgc acaacggttg ccgtccgcca aagcgccgca gagtataaaa
     6841 agatggcgct tacgatgcat ggcgagtgtc agcgttgtaa agcgccgctc tcacccgcgt
     6901 cggaagcgta catctgtact tacgagtgca cgttttgtct ggattgttgg cagacattga
     6961 aaaaaaatct gccctaactg cgggaatgag ctggtccgca ggccgcgcaa ggagttgctc
     7021 gttgccggtt ccaggtgagt tgaaaactgg caaccgagga ctgagaactg taaactaagg
     7081 actggactgg gacgaagcgc tgccagcgcg taaaccggtc ggcctctgaa gtcaaggaga
     7141 agagaattgg cacgttatac cggagcagta tgccgccttt gccgtcgcga aggcgtgaag
     7201 ctttttctga aaggcgcgaa atgcttttca gacaaatgcc cgattgagaa gcgcaacttt
     7261 gcccccggcc agcatggccg cgaccgcaag gcgaaggtgg taggctacgg tctccagctt
     7321 cgtgaaaagc agaaggcgaa gcgcatttac tttaccctcg aaggtcagtt ccgtgagtat
     7381 tacgagaagg cgtcgcgcgc ccagggcgtt accggcgaac tgctcctgca gcagcttgag
     7441 cgccgtctgg acaacgtcgc gttccgtctc ggattcgcat catcgcgccg tcaggcccgc
     7501 cagattgtcc gtcatggaca tgttgaggtc aacgggcgta aggtgaacat cccttcgttt
     7561 caggtaagcg tgggagacga gattaaagtc cgtccgaaca gccagaagct gtctgttgtg
     7621 gagacggcgc gcgagtttgc cagccatcaa ccggccccgg catggctcgc tgtagaccac
     7681 gcgaacctgt cgggcaaggt gctggcgctg ccgaagcgtg aagacatcca gttgccggtc
     7741 aacgaacagc tgatcgtcga actgtatagc aagtaattcg ttgctggttg ccagttaccg
     7801 gaaacagact ggggaccaac tgtagactaa caactctttc aggagaggaa acaggcgcag
     7861 acgccgcaga atgcttcgcg ggtcgcgcca ggaagaagcg aaacgatatg ctttggagag
     7921 gatttcaaaa gccgaaacgg ttggcggtgg atacagacac gctgaccaac acctatggga
     7981 agttcagcgc ccagccattt gagcgcggct tcggaacaac cattggcaat gcgctgcgcc
     8041 gcacactgct ttcgtccatc gaaggcgcgg ccgttaccgc cgtcaagatt gagggcgtcc
     8101 tgcacgagtt ccagtccatt acgggagtcg tcgaggacgc gaccgacatc attctgaacc
     8161 tgaagcagat tccattcaag ctgaacggcg aggggccgaa ggcgctctat gtgcgcgcgg
     8221 agcagccggg cgtagtgact tcaggcatga ttgaagccga cggcgacgtg gaaatcctcg
     8281 acaaagacat ctacatcgct actgtgagcg aaggcggcaa gctggacatg gagatgcgcc
     8341 tgaagcgtgg gcgcggctat gtctccgccg acaagaactt cgattccgat ctcggcatcg
     8401 ggttcatccc ggtggattcg gtccactcgc ctgtacgcaa ggtgaacttc acggtagatg
     8461 cagcgcgtct gggccagatc accgactacg acaagctcac gctggaggtc tggaccaacg
     8521 gctccgtgct tccggcagat gctatcggac tggctgcgaa gctgctcaag gaccacatga
     8581 gcatcttcat caacttcgag gaagagattg aagccgagca gcaggccgaa gagcgggtgc
     8641 agttgcgcaa tgacaatctg aaccgctcgg tcgaagagct ggagctgagc gtgcgcagtt
     8701 acaactgcct gaagaacgcc aatatccaga ccatcgggga gctggtgcag aagaccgagg
     8761 ccgagatgct gaagacgaag aacttcggcc gtaagtccct gaacgagatc aaggagatcc
     8821 tcgcacagat gggcctttca ctcggcatga agattgacga gcacggtaat gctgttcccg
     8881 gaccgaccag tgttctgcct tcagcgcagc ttgcagccag ccgctacaac caatttgacg
     8941 aagaggaagg actggatgag gcagacattc cgctccagtc tgaaccggaa aatttttagt
     9001 cgtgattcgt tgtcagtccc cggttttgag taacttcacc ggggactgac tcctaagaac
     9061 tcataacttg tttttcagag agatttgcca tgcgccatcg caatgcagga tacaaacttg
     9121 gacgcaatac cagccatcgc cgcgcccttc tgcgcaacct ggcgacgtcc gtcattctgg
     9181 aagatcgcgt ggagacgacc gttgccaagg cgaaggctgt gcgtcctcac gtggaaagac
     9241 tgattactct gggcaagaag ggtgacctgc atgcgcggcg tcaggccctt gcttatctgc
     9301 agacccgcga cgcggtcacg cgtctgtttg acactgttgc accgcgctat ggcgaccgca
     9361 acggcggcta tctgcgcatc gtgcgccgcg gttttcagcg cggagacggc gcagagaagg
     9421 cgtttatcga gcttcttggc gccgaggcgg tccttgacga aaagcgccag aagcgcgccg
     9481 aagcacgtca gaaacgccgt gaagagctgc agaaggccat ggaagccgaa cagcagcagg
     9541 ccaacgaagg cggcgaacag gcgtaaacac gttgcttaag acttcaggcg cactttgctt
     9601 tgatgcaaag tgcgcttttg ttttttggat tctgcttccg ggtcatccat agataaacta
     9661 tgtatatggt gagttgttgc gaagtttttt tgtaaattct tgataataaa agaattgatg
     9721 tatttattct ctgctttttg ggtcgctgct gattgccagc ccagtggctg atacccagga
     9781 ttatctatct acacccggat tcagatgggg cggaatcaaa aggcccgtat tttcccggta
     9841 atctgccccg gctcattttg tcgggaatgc tcccggttcg taccccaccg tactggttcc
     9901 accctccccc cctactttgc tggtaaagtc ctgatataaa agctggttac ggaacgcttt
     9961 gtgactcttg tctgtggtga tgcgcttttc ctcgatggcg cggaaagctg cctccggtca
    10021 ggattccggg cctcaatctc gggcaggaca ggtaggggag cacagggcca gcgtctgaag
    10081 gccatccatg tctgggtgtc gtgtatcaac gtcagcgggg tcgagatggc cagactggat
    10141 atcgtccatg agcaacatac tggggtgggt gatctggtct ctatctgcgg ggaggtcttc
    10201 gggatcaggg cactcttttt ccgggccaag gggttctccg gtggagtcaa agaagacttc
    10261 gcggacagat tgaggtgcgg aagcctgggc ggattctctg gtagcgggcg tggttggggc
    10321 agagacggtt ttggcgcgag tgatggcgga gagagcgcga aagtgctggc tggcgatctg
    10381 gagtccatag aggagggagt gggcgcggcg ctggtcgagc acaccggcgg ccagggcgat
    10441 gaccacatcg gagatggcga gctgaatggc ctcggggtcg tcgaggacgg ggaggttgag
    10501 agggtagtct tcgcgggcga tggcgttata agactcggag ccgggtttgc ggcgcttacg
    10561 cgtgttggag tggtaatagc agaagtcatc gcctttgagg gcaggcgagc cgcacttgtt
    10621 tccgccggtg aagatatggc ggcattcttt atacacaggg gcctccaggt tttgagcgga
    10681 agtaaaaaag cccgctttgg gcagcgggct tcggttcgtt ttctctctta aatccagtat
    10741 atcaagccgg agcaggagaa atggacaagg atttgcaggg tgttgtgcct ttggaatgaa
    10801 ggagttatgc cggaaatgga gggctggggg cttgacagct ttccggttac cggccgcgct
    10861 taagtcctga attttgccgc aggcgttcac ttttgtgtaa aaaccgggtt tgtcttttga
    10921 ggggatcttt tgctttatcg cccttttacg caggtctcta taaactaaga cagggcgatt
    10981 tttcgcatct aaaggggaac agagattcgc ccaaactttc ttcctatgaa gttgcatcgg
    11041 ttttgccttc tgatagcctt tctgctggtg gttgcatccc tgaccgctgc cgctgatact
    11101 gcgccgtttg acctggctgg accgaaggtg gatgtgcgca ttacgcgttc cggtaagacc
    11161 cttccgattg gcaaggtacc caatctgcaa gccggtgacc gtctatggat ccatgcgcag
    11221 atgccggccg gtcaatccgt gaaatacctg atggtcgtgg tctttctgcg cggctcgacg
    11281 aatccgccgc cggacaagtg gttttataag gccgagacgt gggacaagaa ggttgcgcag
    11341 gaaggaatct ttgttacggt gccggacggg gcgcagcaag cggtggtctt gtttgctcct
    11401 tcaacgggcg gcgacttcaa taccatccgc gacgcggtgc attcgcggcc tggctcgttt
    11461 gtgcgggcct cgcaggacct gaaccagttg agcctggacc gggcgcgcct ggatgcgtat
    11521 gtagaggccg tccggcagat caatgaaacc aatcctgaca ggctgaagga cgtttcgccg
    11581 ctgctggcgc gcagtctgag catcaagctc gaccaggact gcctgcagaa agtcgtcgaa
    11641 cagcaggcgt catgtctggt gcagaagcag gaccagcttg tgatggacga tgggcacagc
    11701 ctttcagtga cggatgcgct gaccagcggg gcatcgagcg atctggccat gcagttgagc
    11761 tatacgcagc gggccggggc agggtattac agcccgtata tcgcttccgt gatggacatg
    11821 gcgaagatcc tggaagggct gcacactgcg caatatcaat atattcctgc gctgaatatc
    11881 gtaaaaaatg acgaagttga gctgaagctg aatacgccgc cgtcgttccg gaaccctaaa
    11941 tcggtgattg tgatggcgct tcctgcgatt gacaaggggc atctgccgcc actgcgtcct
    12001 gtggatgaaa agcaggtgca gtgcatcgcg aaggacaatc ccgtgttgcc ggctgagggc
    12061 gcgccgcttg actttgccac cgattatgcc cacgatatgt ttctgcgcgt gaagggcaag
    12121 aatggaaagg acctggatat tccagtgaag gccgaacccg cgcatggggg ctttgtggcc
    12181 gatacgcatg gaatttccat ggaaaacatt ggcagcgaca ccggaacgct gcacggatgg
    12241 tggggctttg aacagtacag cggcccggtc tttcatttcc agacaggccg gtcggcaaag
    12301 tgggagctgg ccgcgacgga caagcatgca ctggtggtgg ggcgcgaaga cacgctgcat
    12361 ctgacttcag gcgatgcgac ctgcgtggat tcggtcacgg ccaaggacgc tgcgggaaaa
    12421 gagattgcag ttacgtggaa agcagaccag ccagaccagc ttgaaatcaa agtgccgcta
    12481 aaggatgcga ccccggggcc actggtgctg gcggtgaagc aggcgggggc aaaacagccg
    12541 caggaaatgc acctggaggg actggaagag gcgggaaaat tcgagagctt caccatcaat
    12601 gcgggagaca cacaaggcgt actggcaggg aaccggctgg atgaagtcgc cagcctgaat
    12661 ctgaaaggcg ttattttcaa gcccggaaag ctggccaaca ccgaaaacgg cgatgcactc
    12721 acgctggagg cagcagacag caagtcagct tccgcactgc aggcgggaac gaaaacgacg
    12781 gcgcaggtct ttctgaaaga tggccgagcg ctggatgttc cggcggcggt ggttgctccg
    12841 aggccgaagg tgcaattgct gagcaagagc gtgcagccgg cagagtctgt gaaggccacg
    12901 cacattcagc ttacggacac agacctggtc ccgctggaca gcaaacttgc gttttcagta
    12961 aaggcgatta ctcctgcgac gttctctccc gacgagaaga ttgaagtagc gacggaagat
    13021 ggctccttcc acacgacact gagcatggcg gacgggggga tcacacttca ggatgcaaag
    13081 acggcggtgg tttcgctgga tccggcaaag agctttgggg cgtcggcgtt tgggcctttg
    13141 cgattccgcc ctgtgagcgg aaatgggacg acgggcgact ggcagccgct ggcgacgctg
    13201 gtgcgcctgc cgatgctgga cgcgctgcaa tgctccggtg gaccggggca gacgtgcacg
    13261 ctgaaggggt cgaacctgtt tctgctacag gccgttgccg ctgatgcaca gtttgctcag
    13321 ggcatgcagg taccggacgg gtttgccggg aatgctttgc aggtgccgca tccggtgaac
    13381 gggacgcttt acgtaaaact gcgggatgat ccgaatgcgg tgagcacagc tgtgctgccg
    13441 cgtccggcgc cgcagagagc tgcactggtg gcagcgccgg cgacgagcgc gcagcccgcg
    13501 cctactccgc aacagcaggc acaacctgag cctgaaccgg cggcggccgc accgcaaccc
    13561 caggcgaatg ctccgcatga gtaaggaaag cctcgggttg aggcccaggt ttctctgagg
    13621 ccgggcctgt agcagttcgg ggaatccgca ggttccacat ctttccacag aggatgcacc
    13681 atttctgcgc cttgttccgc tctggattga ggcataggct cgactggtgg gtttttacac
    13741 aggattcatc ttcaggattg gcgcggcttt ttcgcgttct ccacaggaaa agccctgaaa
    13801 taggacaaag agtgcacctt tttgcatcta aaatactaga tgttgtggtt tccacttgac
    13861 acacactatt aacagagcta ccttcacgac tgcggcacta aaatctgtcg agggttccgg
    13921 gcatgatttt gagagtcaac aagccgagaa agcgatgttc gcccggtagg ctgtgttctt
    13981 aaaccagtcc ttctccagag ttcgctttgc atgatttttt ccgggcgtcc agcatcgtcc
    14041 ggagtctgga accgaaaaaa cattcctcca ggaaggagtc atcatggccg aggccacgaa
    14101 aaagatcgcc agttccgtta tcgcctctac agccagccag ccagcatcgg cgctgaagtt
    14161 tgagagggtc ttctcgaaag ccggtgtttc gccctatgac gaagtccagt gggagcgccg
    14221 cacggcctcc attacggacg cgagcgggaa gaccatcttc gagcagaagg acgtggaagt
    14281 cccggtggac tggtctatga cggcgaccaa cattgttgcc agcaagtacc tgcatggcca
    14341 gcttgaaacg ccggagcgcg agacgggagt acgccagctt atttcgcggg tggccgagac
    14401 gatccgcgac tggggcctgg cgggtgggta cttctctgct gaggacgctg cggttttcca
    14461 tgacgagctg gtgcatctgc tggtgaccca gaaggcggcc ttcaattcgc cggtgtggtt
    14521 caacgtgggc tgcgaccgcc tggagccgaa ttcggatgcg cagaactggc actggaaccc
    14581 gcacacgtgc gcggtggagt tttccgtcac cggataccgc aagccgcagt gctcggcctg
    14641 cttcatcaat gcggtggatg attcgctgga ttcgattctg aacctggcca agaccgaagg
    14701 catgttgttc aagtgggggt cgggcacggg cacgaacctt tcgccgattc gcgggtctat
    14761 ggagctgctt tcgggtggcg ggacggcctc cggtccgctg agctttatgc gcgggttcga
    14821 tgcttttgcg ggggtgatta aatccggcgg caagacgcgg cgcgcggcca agatggtcat
    14881 cctgaacgtg gaccatccgg acatcatgga cttcatcgaa tgcaagatga aggaagaagc
    14941 aaaggcctgg gcgctgatgc gtgagggcta tgacggctcc ggcccggaca gcgaggcgta
    15001 ctcgtccatt ttcttccaga acgcgaacaa ctctgtgcgc gtgacggatg agtttatgcg
    15061 cgcggtggag aaagacggcg agttcacgac ttacacggtg aaagaccaca agccggtaaa
    15121 gacgtacaag gcacgcgaga tcatgcagaa gatcgccgat gctacgtggc actgcggcga
    15181 tccgggcatg cagtatgaca ccacgatcaa caagtggcac acgtcgaaga acacagggcg
    15241 catcaatgcg tcgaacccgt gctcggagta catgttcctg gacaactcgg cctgcaatct
    15301 ggcgagcttc aa
//
LOCUS       DASRBV010000230         5431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_369173, whole genome shotgun sequence.
ACCESSION   DASRBV010000230 DASRBV010000000
VERSION     DASRBV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369173"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..200)
                     /locus_tag="VFA02_08670"
     CDS             complement(21..200)
                     /locus_tag="VFA02_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09966.1"
                     /translation="MQNLMNLIVLMCAAVAALAFGVLAAYGICRAAFGVLKIHARSYA
                     KVPQTKPEIAASASR"
     gene            368..3202
                     /gene="uvrA"
                     /locus_tag="VFA02_08675"
     CDS             368..3202
                     /gene="uvrA"
                     /locus_tag="VFA02_08675"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZU09967.1"
                     /translation="MSITHITVRGARQHNLRDINVQIPRNTLTVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVETLSAYARQFLDQVERPEVDSIEGLSPAISIEQKTTSRSPRSTVGT
                     ITEIYDYLRLLYASVGVPHCPNCGRPISRQTQEQIVERILSLAPGERVTVYAPVVRGR
                     KGEFKDLLDELDQQGFRARIDGELRDLAEPVNLEKRKNHTIEAVIDRIVLKPGVEKRL
                     QEAVARSLQMANGLVLVAVSGGGEQLYSSSMACPDCGLDVPKLEPRSFSFNSAYGACP
                     ECHGLGSIYDFDPAKIINDWSKPLLDGALGPGSASQYLVKLIHLAAQRYKIDLKKPFE
                     QLPKKHQDLLLYGPGKAEAPRTGFHGILAYLRDAVEESKSDAYREWMLGYMSASTCPA
                     CQGKRLRPESLAVKVGGLSIADFTALSLKRAVETARGLDFTEREAMIAERLRREVVER
                     LEFLNAVGLGYLSLNRSAATLSGGEGQRIRLATQIGSRLRGVLYVLDEPSIGLHQRDN
                     QRLIHALESLRNLGNTVLVVEHDEDTIRHADYVLDLGPGAGKLGGHVVAEGPPQQIMQ
                     TPESLTGRYLAGEISVLHRLMPRVPNGKWITVEGARSHNLRNITAKFPLGVMTVVTGV
                     SGSGKSTLVNDILYRALAKQLYGSREEPGEHTRILGHENIDKVIRIDQSPIGRTPRSN
                     PATYTGVFSAIRDLFAMLPESRERGYKAGRFSFNVAGGRCEACQGEGQRRIEMNFLPD
                     VYVQCEVCNGRRYNQETLAVKFNGHSIADILDLAIEDALPVLADIPQVKQKLQTLVDV
                     GLGYIHLGQSATTLSGGEAQRMKLARELSKRQTGRTLYLLDEPTTGLHFDDVRKLLEV
                     LHRLTDLGNTIVIIEHNLDVIRNADWIIDLGPEGGEEGGQIVAEGTPERVSRTKGSYT
                     GEFLRRYYGDVSVNGHGTSKGRRARKVTVQ"
     gene            3199..4089
                     /locus_tag="VFA02_08680"
     CDS             3199..4089
                     /locus_tag="VFA02_08680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZU09968.1"
                     /translation="MMLDPVSLTPMETQRPSGEELRPEPLPSEGEIPNLGHAVLFFLM
                     SAPLLLIGESLSLFVAKQILHGKNYQALFDLMSTDARLSIPTQAFVYLLIGFVTVAVF
                     TVLWQRPFGEGIHWNAAIARQRFSWLFMLGIGIGVGITFLQAYLPIPMPQNPPILQDM
                     VHSSLGAWLMLFFGVSIAPLMEELAFRGFLLPGLIHGFRWLMRHQILSAEAFSWMTVP
                     LSILLTTIPFALLHAEQVSRAWGPLLLIGLVSVALCVVRLRLNSLAASTVVHAAYNFT
                     LFAGLLLQTEGFRHLERLNS"
     gene            4180..4692
                     /gene="ruvC"
                     /locus_tag="VFA02_08685"
     CDS             4180..4692
                     /gene="ruvC"
                     /locus_tag="VFA02_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZU09969.1"
                     /translation="MRVFGIDCGTECTGYGVVEWDDTQSGPRLVPLAAGGIRLSKKLP
                     LSQRLALLYAELSALLARHEPDIVAVEEVFYSVNAKSALKLGQVRGVALLAAANANLS
                     VAEYAPLTIKSTVVGYGLAQKEQVQFMVARLLQLEKAPEPADAADALAIAICHIHTTQ
                     TLILQSGAVR"
     gene            4693..5364
                     /locus_tag="VFA02_08690"
     CDS             4693..5364
                     /locus_tag="VFA02_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09970.1"
                     /translation="MKKRMLAFMAIGITAIAFAQDNHQKARIRFIFENPKLQPSTYVM
                     DIYEDGTGHFKSQPGGAAVSDAEGIAVQPLDTDIRIENPLLGVLFKTARSHNFFDVAC
                     ESTKSKVAFTGKKALMYTGPDGDGSCTFNWSRDQQIMKISEDLIATAYTLQVGQRLTI
                     EHEHNRLGLDSELESLQDAAKQGQAQQLQNIAPQLQAIANDDSVMERARGRARQLLSA
                     GKPGN"
BASE COUNT         1108 a         1355 c         1762 g         1206 t
ORIGIN      
        1 tttagctatc cgctttttag ctaacgcgaa gcgctggccg cgatttctgg tttagtctgt
       61 gggactttgg cgtaggagcg cgcatggatt ttcagcactc cgaaggctgc gcggcagatt
      121 ccgtatgcgg cgagcactcc aaaggcgagt gcagccactg cggcgcacat taatacgatc
      181 aggttcatta agttctgcat ttcaaatcct ggcttcccgt aagctccgat gctcattgtc
      241 gcagaaacag aggcgaattg accctgtttt ctattgccaa ctaagatacc tatagtaatc
      301 agttccagtt ttggacttat agtaaccagc ttattgagac cgcgaattct ctgaattttc
      361 acactgcatg tccatcaccc acatcacagt acgcggagcg cggcagcata atctgcgcga
      421 tatcaacgtc cagattccgc gcaacacact gacggtagtg accgggcttt ccggctcggg
      481 caaatcgtct ctggcgtttg acacgatcta tgccgaaggg cagcgccgct acgtagaaac
      541 cttatcggca tacgcgcggc aattcttaga ccaggtggag cggccggagg tggattcgat
      601 tgaggggctg agtcccgcga tttcgattga gcagaagacg acgagccgca gtccgcgctc
      661 aacggttggc acgattacgg agatttacga ctatctgcgt ctgctgtatg cgtcggtggg
      721 tgtgccgcat tgtccgaatt gcggacggcc catctcgcgt cagacgcagg agcagattgt
      781 cgagcgtatt ttgtcgttgg cgccgggcga gcgggtgacg gtttatgctc cggtggtgcg
      841 cgggcgcaaa ggagagttca aagaccttct ggatgaactg gaccagcagg ggttccgggc
      901 aagaattgat ggcgagttgc gtgacctggc cgagccagtg aatcttgaaa agcgcaagaa
      961 ccacaccatt gaggcggtga ttgaccgcat tgtgctcaag ccgggtgtag agaaacggct
     1021 tcaggaagcc gtggcgcggt cgctgcagat ggcgaatggg ctggtgctgg tggcggtttc
     1081 gggcggtggc gagcagcttt attcgtcttc gatggcgtgt ccggattgcg ggctggacgt
     1141 gccgaagctg gagccgcgca gcttctcgtt caacagcgcg tatggggcgt gtccggagtg
     1201 ccacgggctg ggcagcattt acgacttcga tccggcgaag atcatcaacg actggtccaa
     1261 gccgctgctg gatggcgcgc tggggccggg atcggcgtcg cagtatctcg tcaagctgat
     1321 tcatctggcg gcgcagcggt acaagattga cctgaaaaag ccgtttgaac agcttcccaa
     1381 aaagcaccag gaccttctgc tttacggtcc cggaaaagct gaagctccgc gcacagggtt
     1441 ccatgggatt ctggcttatc tgcgcgatgc agtggaggaa tcgaagtcgg atgcatatcg
     1501 cgagtggatg ctgggttata tgtcggcttc gacgtgtcca gcgtgccagg gaaaacgcct
     1561 gcggcctgag tcgctggcag tgaaagtggg cgggctttcg attgcggact ttacggcgct
     1621 atcgctgaag cgagctgtag aaacagcgcg cggcctcgac tttaccgagc gcgaggcaat
     1681 gattgcagag cgcctgcggc gggaggtggt cgaacggctg gagtttctga atgcggtcgg
     1741 gttgggatat ttgtctctca atcgcagcgc ggccacgctc tcaggcgggg aagggcagcg
     1801 catccgtttg gccacgcaga ttggttcgcg attgcgggga gtgctgtatg ttctggatga
     1861 gccttcgatt ggcctgcatc agcgagacaa tcagcggctg attcacgcgc tggaatcgct
     1921 gcgcaacctg ggaaatacag ttcttgttgt cgaacatgat gaggacacga ttcgacacgc
     1981 ggactatgtg cttgatctgg gtcccggcgc agggaagctg ggcggccatg ttgtggcgga
     2041 aggcccgccg cagcagatca tgcagacgcc ggaatcgctg accgggcgtt atctcgcggg
     2101 cgaaatcagc gttttgcacc gtctgatgcc gcgtgttccg aatggcaaat ggattacggt
     2161 agaaggcgcg cgcagccaca atctgcgcaa catcacagcg aagtttcctc tgggcgtaat
     2221 gactgtggtg accggtgttt caggatcggg caagagcacg ctggtgaatg acatcttgta
     2281 tcgcgcactg gcaaagcagc tttatggttc gcgcgaagaa cccggcgagc acacgcgcat
     2341 tctggggcac gagaacattg ataaagtgat tcgcattgac cagtcgccga ttgggcgcac
     2401 gccgcggtcg aatccggcaa cgtatacggg tgtgttttcg gccatccgtg atctgtttgc
     2461 catgctgccg gaatcgcgtg agcgtggata caaggccgga cgcttctcct tcaacgtggc
     2521 cggagggcgc tgcgaggcgt gccagggaga aggtcagagg cggattgaga tgaactttct
     2581 gccggatgtt tacgtgcaat gcgaggtgtg caacgggcgc cgttacaatc aggagacgct
     2641 ggcggtgaag ttcaacggcc attccattgc agacatactt gatctggcga ttgaagatgc
     2701 gctgccggtg ctggccgata ttccgcaggt gaagcagaag ctgcagacgc tggtggatgt
     2761 tggtttgggt tatatccatc tcgggcagtc ggcgacaacg ctcagcggag gcgaagcgca
     2821 gcgcatgaag ctggcgcgcg agctgtcgaa gcggcagacg gggcgcacgc tctatctgct
     2881 ggacgagccg acgacagggt tgcactttga tgacgtgcgc aaactgctgg aggtgctgca
     2941 ccggctgacc gacctgggca acacgattgt gattatcgaa cacaatcttg atgtgattcg
     3001 caatgcggac tggatcattg atcttggtcc tgaaggcggc gaagaaggcg ggcagattgt
     3061 ggcggaagga actccggagc gggtttcacg cacgaaaggc tcgtatacgg gggagtttct
     3121 gcggcggtac tatggcgacg tatctgtgaa cggccatgga acatcgaaag ggcgcagggc
     3181 aaggaaggtc acggtgcaat gatgctcgat ccagtttcgc tgactccgat ggaaacgcag
     3241 agaccgtccg gggaagaact gcggcctgag ccgttgccct cagaaggtga gatacccaat
     3301 ctgggccacg ctgttttgtt tttcctgatg agcgcaccgc tcctgttgat tggggaatcg
     3361 ctcagcctct ttgttgcgaa gcagatactg cacgggaaga attaccaagc attgtttgac
     3421 ctgatgagca cggatgcgcg gctttccatt cctacacagg catttgtgta tctgctgatt
     3481 ggctttgtga cggtggccgt ctttacggtg ctgtggcagc ggccatttgg cgaaggcatc
     3541 cactggaatg ctgcgattgc gcgtcagagg ttttcctggc ttttcatgct gggtattggg
     3601 attggcgtgg ggattacgtt tttgcaggcc tatctgccga ttcccatgcc gcagaacccg
     3661 ccgattctgc aggacatggt gcattcctca ctgggcgcat ggctgatgct gttttttggg
     3721 gtttcgattg ctccattgat ggaagagctt gcatttcgtg gctttctgtt gccgggactg
     3781 attcatggct tccgctggct gatgcggcac cagatccttt ccgcagaggc gttttcatgg
     3841 atgacagtgc cgctttcgat tctgctgacg acgattccgt ttgcattgct gcacgcagag
     3901 caggtttcgc gggcgtgggg gccattgctg ctgatcggac tggtgagtgt ggcgctgtgt
     3961 gtggtgcgtc tgcggctcaa ttcattggcg gcgagcacgg tggtgcatgc ggcgtataac
     4021 tttactctgt ttgcggggct tctgctgcag acggaagggt tccggcatct ggaacggttg
     4081 aatagctagg gtcgtgggca ggacagggtg agtcctgggg ctttgaacga gggcaactat
     4141 cttctttcac ccacacataa tcagctaaac tcatcctcaa tgcgggtttt tggcattgac
     4201 tgcgggacgg aatgcaccgg ctatggcgtg gtggagtggg acgacacgca gagcgggccg
     4261 cggctggtcc cattggcggc gggcggcatc cggctgtcga agaagctgcc gctgtcgcaa
     4321 agactggcgc tgctttatgc cgaactgagc gcgctgctgg cgaggcacga gccggacatt
     4381 gtggctgtag aagaagtctt ttattcggtg aatgcgaagt cggcgctgaa gctggggcag
     4441 gtaaggggag tggcgctgct ggcggcggcc aatgcaaatc tttctgtagc cgaatacgca
     4501 cctctgacca tcaaatccac cgtagtggga tacggactgg cgcaaaagga gcaggtgcag
     4561 tttatggtgg cgcgattgct gcaactagag aaggccccgg agcctgccga tgcggccgat
     4621 gcactggcca tcgccatttg ccacattcac actacgcaaa ctctgatttt acagagcgga
     4681 gccgttcgct agatgaagaa acgaatgctg gccttcatgg ccatcgggat tacagccatc
     4741 gcatttgcgc aggacaatca ccagaaagcg cgtatccggt ttatttttga gaacccaaag
     4801 ctacagccct ccacctatgt gatggacatt tacgaggacg gtacggggca cttcaagtca
     4861 cagccgggag gcgcggctgt ttctgacgcg gaaggaattg cagtccagcc gctcgatacg
     4921 gacatcagga ttgaaaatcc actgctgggg gtgttgttca agactgcgcg ttcgcacaac
     4981 tttttcgatg tggcgtgtga aagcacgaag tccaaagtgg cgtttacggg caagaaggcg
     5041 ctgatgtaca cggggccgga tggcgatggt tcgtgcactt tcaactggtc gcgcgaccag
     5101 cagatcatga agatttccga ggacctgatt gctaccgcgt atacgctgca ggtcggccag
     5161 cggctgacga ttgagcatga gcacaatcgc ctggggctgg attccgagct ggagtccctg
     5221 caggatgcgg ccaagcaggg gcaggcacaa caattgcaaa atatcgcgcc gcagcttcag
     5281 gccattgcga atgacgacag cgtgatggag cgggcgcggg gccgggcacg tcagttgctg
     5341 agcgcaggga agccggggaa ctgaggagat gctcacctct acctttcccg taaatcgcac
     5401 tgcgctggaa gtggccgtca tccatagata a
//
LOCUS       DASRBV010000231         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_318965, whole genome shotgun sequence.
ACCESSION   DASRBV010000231 DASRBV010000000
VERSION     DASRBV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318965"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFA02_08695"
     CDS             <1..207
                     /locus_tag="VFA02_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZU09971.1"
                     /translation="RGFLETASHLRLIAAGGLNPVNVAEAIQTLGPWGVDVASGVEAA
                     PGKKDPALVKAFVSAARAGAAIIA"
     gene            229..1443
                     /gene="trpB"
                     /locus_tag="VFA02_08700"
     CDS             229..1443
                     /gene="trpB"
                     /locus_tag="VFA02_08700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZU09972.1"
                     /translation="MSTTQLIQSASTGRFGPYGGRYVPETLMAALEELEHAYAQAQAD
                     PAFQAELDSLLRDYAGRPTPLYYASRLTEKLGGAKIYLKREDLLHTGAHKINNCIGQG
                     LLAKRMGKKRIIAETGAGQHGVATATVCALLGLECVVYMGEEDMQRQELNVYRMRLLG
                     AEVRGVSSGSRTLKDAINEAMRDWVTNVRTTHYLLGSVLGAHPYPMMVRDFHRIISKE
                     ARKQILEKEGRLPSAVIACVGGGSNAIGAFYEFIPDKEVRLIGVEAGGRGTKLGEHAA
                     RFQGGSPGVLQGTYSYVLQDDAGQIALTHSVSAGLDYASIGPEHAMLHDTGRAEYTSA
                     SDEDALNSTVTLARTQGILPALESAHAVAECIRLAPTLGRHEILVVNLSGRGDKDMGI
                     LSRELKLKGATE"
     gene            1453..2241
                     /gene="trpA"
                     /locus_tag="VFA02_08705"
     CDS             1453..2241
                     /gene="trpA"
                     /locus_tag="VFA02_08705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZU09973.1"
                     /translation="MPIRFNQKPGLVVYLTAGDPDLATTHEIALAAIDAGADVLELGV
                     PFSDPLADGPVIQRASERAVRNGTKLSDVLALAAELRSERPQAGLVIFSYYNPVLRFG
                     LESFCEAAEKAGVDGVLITDMIVEEADEYLRHLQTHHLAPVFLAAPTSPDERLERIAK
                     ASQGFVYAISRTGITGTQQQLASDAEQLVKRLRRFTQLPIAVGFGISNAEHVAAVGEF
                     ADAAVIGSAIVALIEKSAREEAPSSIARFIKGLRQPVALAQSSR"
     gene            2310..2621
                     /locus_tag="VFA02_08710"
     CDS             2310..2621
                     /locus_tag="VFA02_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZU09974.1"
                     /translation="MDIADWRKKIDEIDRQIVALISQRAEAAQAIGKLKKSTNLPVYE
                     PNRERIIFDNVRAANKGPLPDIELVHIYERIIDVMRALQQDELASQRSGPSAGETGKK
                     S"
     gene            2618..3637
                     /gene="aroF"
                     /locus_tag="VFA02_08715"
     CDS             2618..3637
                     /gene="aroF"
                     /locus_tag="VFA02_08715"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZU09975.1"
                     /translation="MIVAMQEQATEEQIQHVTERMMELGFKVHRTTGTVQSILAGVGT
                     PVAFDVKDFQVMDGVAEAYRITSPYKLAGRGFRPEGTVIKFKNGVEIGNSEAVVMAGP
                     CSVENRDQIFTAAEQVKAAGAKFLRGGAFKPRSSPYSFQGLGLEGLKLLREVGDATGL
                     LIISEVMEISQIELMMPYVDTFQVGARNMQNFNLLRELGQVRKPVLLKRGIAATIEEL
                     LLSAEYILSGGNYEVMLCERGIRTFETYTRNTMDISAIPVLKKLSHLPVVADPSHGVG
                     LRDKVPAMARAAIAAGADGLLIEVHPNPEKAASDGAQTLFPEQFSKLMQELRLIATAV
                     GRNIA"
     gene            3637..4533
                     /locus_tag="VFA02_08720"
     CDS             3637..4533
                     /locus_tag="VFA02_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZU09976.1"
                     /translation="MAIKRIAILGTGLIGGSVGMALRQNGFTGTIFGWDREVVQAAIA
                     LARETITEIADDPIRAAQQSDVVLLSGPVFSILEWLEKLAPILRPDQLVTDVGSVKGI
                     LCERASGLYNQPDKAGFLPGHPMAGKEVSGAANADGSIFHGAVWLFTEGTKRAGMSAE
                     ARAKAEEWCEWVHRFGCRTIDLDPKRHDELCAWVSHLPQFVSTALSALLEERFRDDED
                     LRAIGGRALREMTRLGASPYSMWRDIAHTNEEAVAASLHALEQRLTHLRENLKSPALR
                     EEFDLANRFRLGRPKSIRPSKM"
BASE COUNT         1050 a         1325 c         1396 g          932 t
ORIGIN      
        1 cgcggcttcc tcgaaactgc ttctcatctg cgattaatcg ctgcgggtgg attgaatcca
       61 gtaaatgtgg ctgaagccat tcagacgctg ggaccctggg gggtagatgt ggcgagcggt
      121 gtggaggctg ctccggggaa gaaagacccc gcactggtaa aggcattcgt gagcgcagcc
      181 agagctggtg ccgccatcat agcgtaatct gaaatacacg aggaaattgt gagcacaacg
      241 caactcatcc aatcggccag tacaggacgc ttcggccctt atggcgggcg ttatgttccg
      301 gaaacgctaa tggcagcgct tgaagaactg gagcacgcct atgcgcaggc gcaggctgat
      361 cctgcctttc aggccgagct ggattcgctg ttgcgcgatt acgcggggcg tcccacgcca
      421 ctgtattatg catcacgcct gactgaaaag ctgggcggag caaagattta tctcaagcgc
      481 gaagaccttc tgcacacagg cgcgcacaaa atcaacaact gcatcgggca gggcctgctc
      541 gcaaagcgca tgggcaagaa gcgcatcatt gccgaaactg gcgcgggaca gcatggagtg
      601 gcaaccgcca cagtatgcgc gttgctcggt ctcgaatgcg tcgtctacat gggcgaagag
      661 gacatgcagc ggcaggagct gaatgtctat cgtatgcggc tgctcggtgc ggaagtccgc
      721 ggcgtcagct caggctcgcg tacgctgaaa gacgccatca atgaggcgat gcgcgactgg
      781 gtcacgaatg tccgcactac acactatctg ctgggcagcg tgctaggggc gcatccgtat
      841 ccgatgatgg tgcgtgattt tcatcgcatc atcagtaaag aggcgcgcaa gcagatcctg
      901 gaaaaagaag gccgtctgcc cagcgctgtc attgcctgcg taggcggcgg ctcaaacgcc
      961 atcggcgcat tctacgaatt tattccggac aaagaagtac gactcatcgg tgtggaggcg
     1021 ggcggacgcg gcacaaagct tggtgaacac gcggcgcgct tccagggcgg atcgcccgga
     1081 gttctgcaag gcacttattc ttacgttttg caggacgacg ctggacagat cgctctgacg
     1141 cattccgtct ccgctggact cgattacgca tccattggcc ccgagcacgc gatgctgcac
     1201 gacacgggcc gcgcggaata cacatcggca tccgacgaag atgctttgaa ttccacggtg
     1261 acgctggcac gcacgcaagg cattctgccg gcgcttgaaa gtgcacacgc cgtggcggag
     1321 tgcatccggc ttgcgccaac actggggcgg catgaaatac tggtagtcaa cctctcagga
     1381 cgtggcgaca aagacatggg tattctttcg cgcgaactga agttaaaggg cgcaacggag
     1441 taagactgtt tcatgccgat tcgcttcaac cagaaaccag ggttggtggt ttatctgacg
     1501 gccggagacc cggacctcgc cacaacgcac gagattgcgc tggccgcgat tgacgcgggc
     1561 gcggacgtgc tcgaattggg tgtgcccttc agcgacccgc ttgcagacgg ccctgtcatc
     1621 cagcgcgcga gcgaacgcgc tgtacgcaac ggtacgaagc tcagcgatgt tcttgctctg
     1681 gctgcggagc ttcggagcga acgccctcag gctggccttg tcatcttctc ctactacaat
     1741 cctgttcttc gtttcggact ggagagcttt tgcgaagccg cagaaaaggc cggggttgac
     1801 ggcgtgctca tcacggacat gattgtggaa gaagctgatg agtatctgag gcatctgcaa
     1861 acgcatcatc tcgcgcctgt ttttctcgcc gcgccgacca gcccggatga gcggctggag
     1921 cgcatcgcta aagcctcaca aggatttgtc tacgccatct cgcgcacggg cattaccgga
     1981 acgcagcagc agcttgcttc cgatgctgag cagcttgtga aacgtctgcg cagattcacg
     2041 caattgccca tcgccgtcgg cttcggcatt tccaacgcag agcacgttgc cgctgtgggt
     2101 gaatttgcgg atgcagccgt gattggcagc gcaattgtgg cgctgattga gaagtctgcg
     2161 cgcgaagaag cacccagctc gatcgcgcga tttatcaagg gcctgcgcca acctgtggcg
     2221 ctggcccagt catcccgcta agtttctaaa aggcagcttg aatttccggt gcaggcactt
     2281 tcgcgtgcgc tccaaaagga aaggcataca tggacattgc agactggcgc aagaaaattg
     2341 atgagattga ccgtcagatt gtggcgctga tcagccagag ggcggaagcc gcgcaggcaa
     2401 tcggaaagct gaagaagtcc acaaacctgc ccgtgtatga gccaaaccgc gagcgcatca
     2461 tcttcgacaa tgtgcgcgca gccaacaaag gccccctgcc cgacattgag cttgtgcaca
     2521 tttacgaacg catcattgac gtgatgcgcg ccttgcagca ggacgagctg gcctcacaaa
     2581 gatcaggacc atccgcaggg gagacaggaa agaaatcatg attgttgcca tgcaggaaca
     2641 ggcgaccgaa gagcagatac agcacgtaac cgaacgcatg atggagcttg gcttcaaggt
     2701 ccatcgcacc acaggcacgg tgcagagtat tctggccgga gtcggcaccc cggtcgcatt
     2761 cgacgtaaaa gattttcagg taatggatgg cgtggcagaa gcctaccgca ttacatcgcc
     2821 ctataagctg gccggacgcg gcttccgccc ggaaggcact gtcatcaagt tcaaaaatgg
     2881 cgtggagatt ggcaactctg aggccgttgt aatggcaggt ccctgctctg tcgaaaaccg
     2941 cgatcagatt ttcaccgcgg cagaacaggt aaaggccgca ggcgcaaagt tccttcgcgg
     3001 aggcgcgttc aagccgcgca gctcaccgta ttctttccag ggactcggcc tcgaaggcct
     3061 caaactgctg cgcgaagtgg gcgacgccac cggactgctc atcatcagcg aagtcatgga
     3121 gatttcgcag atcgaactga tgatgcctta cgtggatacc tttcaggtag gcgcgcgcaa
     3181 tatgcagaac ttcaacctgc tgcgtgaact ggggcaggtg cgcaagcctg tcctcctgaa
     3241 gcgcggcatc gctgctacga ttgaggaact gctgctctct gcggaataca tcctttcagg
     3301 cggaaactac gaagtgatgc tctgcgagcg cggcattcgc acttttgaga catatacacg
     3361 caacacgatg gacatctcgg ccattccggt gctcaaaaaa ctttcgcacc tccctgtcgt
     3421 cgctgatccg tcgcatggcg tcggcctccg ggacaaggta ccagccatgg cccgcgctgc
     3481 catcgccgca ggcgcagatg gattgctgat tgaggtccac cccaatcctg agaaagcggc
     3541 ttcggacggc gcacagacgc tcttcccaga gcagttttcg aaactgatgc aggagcttcg
     3601 cctcattgca actgccgtag ggagaaacat cgcataatgg ccatcaagcg cattgccatt
     3661 cttggaacgg ggctgatcgg cggatccgtc gggatggccc tgcgccagaa tggcttcacc
     3721 ggaacgatct ttggatggga ccgcgaggtc gtacaagcgg ccattgcgct cgcccgcgaa
     3781 acgattactg aaattgccga tgatccaatc agggccgcgc agcagagcga tgtcgttctg
     3841 ttgagcggcc cggtcttcag cattctggag tggctggaaa aacttgcgcc cattctgcgg
     3901 ccggaccagc ttgtgacgga tgttggtagc gtgaagggta tcctgtgcga gcgtgcttcc
     3961 ggcctctata accagccgga caaggccggg tttctccccg gacaccctat ggccggaaaa
     4021 gaagtgagtg gcgctgcaaa cgccgatggc agcatctttc atggcgcagt ctggctcttt
     4081 accgaaggta cgaagagagc agggatgtct gccgaagcac gtgccaaagc agaggaatgg
     4141 tgcgaatggg tgcatcgctt tggctgccga acaattgacc ttgatccgaa gcggcacgac
     4201 gaactctgcg catgggtcag ccatctgcca cagtttgttt ccactgccct gagcgcgctg
     4261 cttgaagaaa gattccgtga cgatgaagac ctgcgcgcga ttggtggacg cgccttacgc
     4321 gagatgacgc gcctcggcgc cagcccctac tctatgtggc gcgacatcgc gcataccaat
     4381 gaagaagctg tcgcggcaag cctccatgcg ctggagcaac ggctcacaca tttgcgcgaa
     4441 aatctgaaaa gtcccgctct gcgcgaagaa ttcgacctgg ccaaccggtt tcgcctgggg
     4501 agaccaaaaa gtatccgacc aagtaagatg taggccatgc ggaaaattgg tctgaccact
     4561 gcccttcttt tcatttttca cgccgtgctt cctctgcgtg cccaacagcc cacgacgctt
     4621 cttgtagatg tagaccatcg ccagcagata agcctgaatg gcccgtggca ctacattgtg
     4681 gatccgtatc atcagggctg ggg
//
LOCUS       DASRBV010000232        12369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_419894, whole genome shotgun sequence.
ACCESSION   DASRBV010000232 DASRBV010000000
VERSION     DASRBV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419894"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFA02_08725"
     CDS             complement(<1..446)
                     /locus_tag="VFA02_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09977.1"
                     /translation="MLIPLHHLRKTLLALFLICAPIGAQQTTELQRLINAEQAARDSG
                     DPSQVISAAQKLTDAAKQQIQTTETALKQPNLTPAKKKQLQTREHLLREVLSTGLNDW
                     GTAEARQRQYAQALEHFQQAESWDASTPGLMRNLGMAAFRLQNYPEA"
     gene            complement(596..4786)
                     /gene="rpoC"
                     /locus_tag="VFA02_08730"
     CDS             complement(596..4786)
                     /gene="rpoC"
                     /locus_tag="VFA02_08730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZU09978.1"
                     /translation="MYRSSPFELTSPITDFDAIRISLASPEKIRSWSHGEVTKPETIN
                     YRTFKPERDGLFCARIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTLSKVRRERLG
                     HIELASPCSHVWFFKGLPSRIGHLLDISLRDLESVLYFESYVVVDPGDSPTREREIIK
                     DENRFRELDQQYRPTGFKAMMGAEAIKELLKRVNVDELAIELRERMKAETSLQKRLKY
                     SKRLKVVEAFRKSGNKPQWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKKLMDLHAPEVIVRNEKRMLQEAVDALFDNGRRGRVLRGANNRPLKSLSDTLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYHRLEQTGHC
                     TTIKQAKEMVELQEPIVWDILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKI
                     HPLVCTAFNADFDGDQMAVHIPLSPEAQVEASVLMLSSHNILSPASGQPITVPTQDMV
                     LGLYYLTKSKKGAKGEGRVFANIEEVLMALNAKEVETLTPIRLRYSGPVLDMTTAYDD
                     QDLLHTEPVEYDKQYISTTVGRAILNDALPEGMPYVNGLLKKKGIGQLVNYCYLNLGL
                     ETTVQMLDRIKELGFQYATRSGLSVGLDDMVIPETKYSLVRDAEKQRFAVQQQYLDGA
                     ITNGERNNKVIQLWSAVTEKVADEMFDNMKNADKEGAMNPIYIMADSGARGSKQQIRQ
                     LSGMRGLMAKPSGEIIETPITANFREGLTVLEYFISTHGARKGLADTALKTADSGYLT
                     RRLVDVAQDVIVSEHDCGTVEGIYVTPIVESGEIIEPLRDRIIGRVSLEKIKDYEGNV
                     IVDVNQEINEDLASQIQAAGIERVKIRSVLTCESRRGVCVLCYGRNLGSGRLVELGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGTASRVSEQSRLDAKNAGTVRFINMVTVRSKQGD
                     LVAMNRSGSIAVIDERGREKERYQVVYGAKLKVEDGQKVELGQAMVEWDPYTFAILTE
                     IGGTVQFKDLQEGITLHEEVDEVTGLSRLVVGDSPDEKRQPAIVIKGAKGNKRYLMPS
                     RAHLMIQDGDEVFPGDVLAKIPRETTRTKDITGGLPRVVELFEARKPRETAIISEIDG
                     VVKFGEVTKGQRKIYVTADNGEEKEYSVPRGVHVNVQEGERLRAGEPLMDGPLNPHDI
                     LAVLGEKELQAYLVNEIQEVYRLQGVAISDKHIEVIVRQMLRWVKIEEVGDTNFLLEQ
                     QVDKFRFRQENERAIANGGRPATGRPLLLGITKASLSTESFISAASFQETTRVLTEAS
                     INGAVDNLRGLKENVIVGRLIPAGTGMEYYRNVQLSPELEEAAARVQQEVHEAYEAAE
                     RELEMMRQEGEAEEMAAE"
     gene            complement(4950..9434)
                     /gene="rpoB"
                     /locus_tag="VFA02_08735"
     CDS             complement(4950..9434)
                     /gene="rpoB"
                     /locus_tag="VFA02_08735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZU09979.1"
                     /translation="MPNENRAIRSRLDFSKIPTAIKIPNLIEVQRRSYERFLQMDKLP
                     SEREDNGLQSVFTSVFPIADFRNISQLDFVDYSIGNWECKCGHLKGLHHLRTTCVNCG
                     AMVITDPFHPGDVLCHKCGTYNKNTPDFCNKCGDPVGLQLKYDQSECEERGMTYSAPL
                     KVTVRLTIYDKDAETGAKTIRDIKEQEVFFGDIPLMTQNGTFIVNGTERVIVSQLHRS
                     PGVFFETANNRTYFLGKIIPYRGSWVEFEYDQKNTLYVRIDRKRKFLGTIFLRALGLK
                     SDEEILKTFYTVDKISVRDNKLFWAVEQDTEKGTHLLGAKPAHAIVAKGEEIAHSGRK
                     ITPSILKALRQHKVSEVEIETAELDGAVTAADVIDTTTGEVFLEANQELTADKLHKIM
                     QSGVSTIEVFFPERDDVGNVITNTLRRDSVRKPEEALIEIYRKLRPGDPPTLDTATAL
                     FEGMFFDPRKYDFSRVGRLKFNIKLYENQDATPLDRRTLTPDDFYATIRYLLKLRKNI
                     GIVDDIDHLGNRRVRAVGELMENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLINAK
                     PVMAAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTH
                     YGRICPIETPEGPNIGLISSLSCFARINEYGFIESPYRRVKDGRVLDYVQITNAGESG
                     LRVGDHLEKQEAIKLNNQLKKDKKRTIEYEPFSFYLSAWEEDRHTIAQANIDLDDEGN
                     IKDELVNARRQGNFVLVNRSEVDYVDVSPKQLVSVAASLVPFLEHDDANRALMGANMQ
                     RQSVPLLVSEAPLVGTGMEGVTARDSGAVVLARRNGIVDSVDSERIIVRVEGEHHPTQ
                     LSREVGSDIYQLIKFKRSNQNTCINQKPIVRQGDRVVKGQVIADGPCTEQGELGLGRN
                     VLVAFMPWRGYNFEDAILISEKLVREDYYTSVHIEEFEIEARDTKLGPEEITRDIPNV
                     SEHALRDLDESGVIRIGAKVKHNDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRD
                     ASLTCPPGIEGTVVDVRIFSRKGQEKDERAKLIEAEQVAKLEKNLADEIRILTDERLK
                     RLEAILGGKEVLADLHDERTNKRLLTKGTILDRDTIELISTRNLKRIRYADKDPRVNE
                     QIDEIEEMTSRQIDVLRKITNEKIAKLQKGDELAPGVIKLVKVYIAMKRKLSVGDKMA
                     GRHGNKGVISRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLGWAAHEVG
                     QQVAAMVNQYRDPARLREVVKEKFANTAVLRQLLELEDEQLIRVCAGMEKGAWFGTAV
                     FDGAKETEIKALLKAAGLPESGKIHLYDGMTGEQFEQPATVGYIYMLKLSHLVDDKIH
                     ARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYILQELLTAKSDDVYGRT
                     KIYEAIVKGEAAIEPGVPESFNVLVRELQALCLDVELIKQVGDEAKKQPLPTFVAAAD
                     "
     gene            complement(10183..10566)
                     /gene="rplL"
                     /locus_tag="VFA02_08740"
     CDS             complement(10183..10566)
                     /gene="rplL"
                     /locus_tag="VFA02_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZU09980.1"
                     /translation="MADLQQLEEQIVGLSLLDAAALVKKLEERLGVSAAAAAPVVVAG
                     AGAGAGAAAPAAAEQTEFTVILKDAGANKINTIKAVREVTSLGLKEAKDLVDGAPKTL
                     KENVSKEDAEAIKKKFEGVATIEIK"
     gene            complement(10720..11256)
                     /gene="rplJ"
                     /locus_tag="VFA02_08745"
     CDS             complement(10720..11256)
                     /gene="rplJ"
                     /locus_tag="VFA02_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZU09981.1"
                     /translation="MALTKAKKAEKVSQLARELQGSTSAIIGTFAKLTVAQDFELRKT
                     VRAAGGRYRVLKNKLAARAAQGTQIEAALQGLKGVSSVAYTSGDPVALAKALSNWVAE
                     NAEFTFKLGIIDGKVITVDEIKQLATMPGKEEIFSKLLFLINAPAQRLATVINATGRD
                     LAVVINQAVEQKKFAEAS"
     gene            complement(11357..12067)
                     /gene="rplA"
                     /locus_tag="VFA02_08750"
     CDS             complement(11357..12067)
                     /gene="rplA"
                     /locus_tag="VFA02_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZU09982.1"
                     /translation="MARRVSKNTAKARAAVEKRPYALQEAVPLLQKVKFAKFDETVDL
                     TLRLGVDPKHADQMVRGTVVLPHGLGKTKKVAVITSGEKLREAEAAGADIVGGEELVE
                     KIQKESWTDFDALIATPDMMKSVGRLGKVLGPRGLMPNPKTGTVTTDVAAAIKEIKAG
                     KVEFRTDKTALVHVPVGKISFPAEKLVENALTVITSVVRAKPSAAKGKYIKGITLSST
                     MGPGIPLDGAIADQVAKA"
BASE COUNT         2499 a         3705 c         3353 g         2812 t
ORIGIN      
        1 gcctccggat aattttgcag gcgaaaggcg gccattccga gattgcgcat aagccctgga
       61 gtggatgcgt cccacgattc ggcctgctgg aagtgttcca gtgcctgagc gtattgccgc
      121 tggcgcgctt ctgctgtgcc ccagtcattc aacccagtgc tcaggacttc ccgaagtagg
      181 tgttcgcggg tttggagttg cttctttttt gccggggtga gattcggttg tttgagcgca
      241 gtttcggttg tctgtatctg ttgtttagca gcatcagtca gcttctgagc ggcggaaatg
      301 acctgggacg gatcgcctga gtctcgggca gcctgttcag cgttgatgag tcgctggagt
      361 tctgttgtct gctgggcgcc gatgggagcg cagatgagga agagcgcaag cagagtcttc
      421 ctgagatggt gtaaagggat gagcatctgt tttgtggctg cctcttctta cacaaacagg
      481 atatagaaca agcagtatag tgagggaagc cgcggttcgt tgtatgttcg aatggagact
      541 tgagataaaa aagccccggc aggaccgggg cttttgattg cgtgaacggc cagttttact
      601 cggcggccat ttcttcggct tcgccttcct gacgcatcat ttcgagttcg cgctcggcgg
      661 cctcgtaggc ttcatgtacc tcctgctgga cgcgggctgc cgcttcctca agctcaggtg
      721 agagctggac attgcggtag tattccatgc ccgtgccggc cggaatcagg cggccaacga
      781 ttacgttctc cttgagtccg cgcaggttgt ccactgctcc attaatggag gcttcggtaa
      841 gaacacgcgt ggtctcctgg aagctggccg ccgagatgaa gctctcagta gacagcgacg
      901 ccttggtgat gccgagcagc agcggacggc cagtagcagg gcgtccacca ttggcaatgg
      961 cacgctcgtt ctcctggcgg aagcggaact tgtccacctg ctgttcgagc aggaagttgg
     1021 tgtcgcctac ttcctcgatc ttcacccagc gcagcatctg acggacgatg acttcgatgt
     1081 gcttgtccga gatggccaca ccttgcaggc ggtagacttc ctggatttca ttcaccagat
     1141 aagcctggag ttccttctca ccgagcacgg caagaatgtc gtgcgggttc agaggaccat
     1201 ccataagcgg ctcaccggcg cgcaggcgtt cgccctcctg cacgttgacg tggacaccgc
     1261 gaggaacgga gtattccttc tcctcgccgt tgtctgccgt aacgtagatc ttacgctggc
     1321 ccttggtgac ttcgccgaac ttcacaacgc catcaatctc agagatgatg gcggtttcgc
     1381 gcggcttgcg ggcctcgaaa agttccacta cacgcggcag accgccggta atgtccttgg
     1441 tgcgcgtggt ttcgcgcgga atcttagcca gaacatcgcc tgggaagact tcgtctccgt
     1501 cctgaatcat gaggtgggcg cgagaaggca tcagatatcg cttgttgcct ttcgctccct
     1561 tgatcacgat ggcaggctgg cgcttttcat ccggcgaatc gcccacaacc agacgcgaga
     1621 ggccggtgac ctcgtccact tcttcgtgca atgtaatgcc ttcctgcaga tctttgaact
     1681 ggacagttcc gccgatctcc gtgaggatgg caaatgtgta ggggtcccac tcgaccatgg
     1741 cctggcccag ctcaaccttc tgcccatctt caaccttcag cttggcacca tagaccacct
     1801 ggtagcgctc tttctcgcgg ccacgttcgt caattacggc gatggagccg gagcgattca
     1861 tggccaccag gtcgccctgt ttggagcgga ccgtaaccat attgatgaag cgcaccgtgc
     1921 cggcattctt ggcatcgaga cgcgactgct cagagacacg tgacgccgtt ccaccgatgt
     1981 ggaaggtacg catggtcagc tgtgttcccg gctcgccgat ggactgtgca gcgatgacgc
     2041 ctacggcctc gcccaactca accagacggc cagagccgag gttgcgcccg tagcagagta
     2101 cgcagacacc gcggcgcgac tcacaggtga gcaccgagcg gatcttgacg cgctcaatac
     2161 ctgcagcctg aatctgcgaa gcgagatctt cgttgatctc ctgattcaca tcaacgatga
     2221 cgtttccttc gtagtccttg attttttcaa gggacacgcg gccgatgatg cggtcgcgca
     2281 gcggctcgat gatctcgccc gactcgacga taggcgtcac gtagatgccc tcgaccgtgc
     2341 cgcaatcatg ctcgctgacg atgacgtcct gcgccacgtc taccaaacgg cgggtcaggt
     2401 agcctgagtc agcggtcttg agggcggtat cggccagacc cttacgtgca ccgtgcgttg
     2461 aaatgaagta ttcgagcaca gtaaggcctt cacggaagtt cgccgtgatg ggtgtttcga
     2521 taatttcacc tgaaggcttc gccatcagtc cgcgcatacc agagagttga cgaatctgtt
     2581 gcttcgaacc gcgggcgccg gagtcggcca tgatgtagat cgggttcatg gccccttcct
     2641 tgtccgcgtt cttcatgttg tcgaacatct cgtcagcaac tttttctgtc accgccgacc
     2701 acaactgaat gaccttgttg ttgcgttcgc cattggtgat ggcgccatcc agatactgct
     2761 gctgcacggc gaaacgctgc ttttcggcat cgcgtacaag cgagtacttg gtttccggaa
     2821 tgaccatgtc atccagaccc accgaaagac ccgagcgcgt cgcatactgg aagccgagtt
     2881 ccttaatgcg gtccagcatc tgcaccgtgg tctcaaggcc aagattcaga tagcagtaat
     2941 tcacaagctg gccaatgccc ttcttcttaa gaagaccatt gacatacggc atcccctccg
     3001 gcagagcgtc attcaggatg gcgcggccta ccgtagtcga gatgtactgc ttgtcgtact
     3061 cgacgggttc ggtgtgcagc agatcctgat cgtcgtaggc cgtggtcata tcaagcacag
     3121 gcccggagta gcggaggcgg atcggcgtta gcgtctccac ttctttcgca ttcagggcca
     3181 tcagcacttc ttcgatgttg gcgaagacgc ggccctcgcc cttcgcgccc ttcttggact
     3241 tggtcaggta gtacaaacca agcaccatgt cctgtgtggg cactgtgatt ggctggcccg
     3301 atgcgggcga cagaatgttg tgcgaggaaa gcatcaacac gctggcttcc acctgcgctt
     3361 caggcgacag cggaatgtgt acagccatct ggtcgccatc gaagtccgcg ttaaaggcag
     3421 tgcataccaa cgggtggatc ttaatggcct tgccttccac cagcaccggt tcaaaggcct
     3481 gaatccctaa acggtgcagg gttggagcac ggttcagcag caccgggtgg tccttaatca
     3541 cttcctcaag gatgtcccac acgatcggct cctgcagctc gaccatctcc tttgcctgct
     3601 tgatggtggt gcagtgtccg gtctgttcta gacggtggta gataaagggc ttgaacaact
     3661 caagcgccat cttcttcggc agaccgcact ggtgcagctt cagttcaggg ccaacaacga
     3721 tcacagaacg tccggagtag tccacacgct tgccgagcag gttctggcgg aagcgtccct
     3781 gcttgccttt cagtgtgtca gagagcgact tcagcggacg gttgtttgcg ccgcgcagca
     3841 cgcgtccgcg gcggccattg tcaaacaacg cgtcaaccgc ttcctgcagc atgcgctttt
     3901 catttcgcac gatgacctca ggcgcatgga ggtccatgag cttcttgagg cggttgttgc
     3961 ggttgatgac gcgacggtag agatcgttca ggtcagacgt ggcaaagcgg ccgccatcca
     4021 gaggcaccag cgggcgcagt tccggtggaa tgaccggaat cacgtccaga atcatccact
     4081 gcggcttgtt gcccgacttc cggaatgcct cgaccacctt caagcgcttc gagtacttga
     4141 ggcgcttctg gagcgacgtc tcagccttca tgcgctcgcg cagctcaatg gcaagctcat
     4201 ccacattgac gcgcttcagc aattccttaa tggcttcggc gcccatcatg gccttaaagc
     4261 cagtggggcg atactgctgg tcaagctcac ggaagcggtt ctcgtccttg ataatctcgc
     4321 gctcgcgcgt aggcgagtcg cctggatcta ccacgacata gctctcgaaa tagagcacac
     4381 tttcaagatc acgcagtgaa atatcgagta ggtgtccgat acgtgacggc agccccttaa
     4441 agaaccatac atgcgagcac ggcgaggcca gctcgatatg gccaaggcgc tcacggcgca
     4501 ctttggacag cgtgacttcc acaccgcact tgtcgcagat gactccgcgg tgcttcatgc
     4561 gtttgtactt gccgcagaga cactcccagt ctgtgaccgg gccaaagatg cgggcgcaga
     4621 acaatccatc gcgctccggt ttgaaggtgc ggtagttgat ggtttccggc ttggtgactt
     4681 cgccatgaga ccagctgcgg atcttctcgg gcgaagcgag cgagatgcgg atcgcgtcga
     4741 aatccgtgat cgggctggtt aattcgaatg ggctggaacg gtacaaggtg accctcctgt
     4801 gcagccttta gctatcagct tccagctgct cttcacttct gttcatatct acgcagcgca
     4861 aagatcgcgc tgctcaatct taagtagcca gttgttaatt gccagccctc agtatgttca
     4921 actgaggact ggcaattgtt cactaacaac taatctgctg ccgccacaaa cgtcggcagc
     4981 ggctgtttct tggcttcatc gccgacctgc ttaatcagtt ccacatcgag gcaaagcgcc
     5041 tgcagctcgc gcacaagcac gttgaatgac tccggaacac ccggctcaat tgccgcttcg
     5101 cccttgacga tggcctcata gatcttggta cggccataaa catcatcgga cttggcagtg
     5161 agcagctcct gcaggatgta cgctgcgcca taggcttcga gcgcccagac ttccatttct
     5221 ccgaagcgct gcccgccgaa ctgcgccttg ccacccagcg gctgctgcgt aataagcgag
     5281 tacggcccaa tcgaacgggc gtggattttg tcatccacca aatgcgagag cttgagcatg
     5341 tagatgtaac ccaccgttgc cggctgctcg aactgctcgc ccgtcatgcc gtcgtagaga
     5401 tggattttgc ccgactccgg caatccggcc gctttcagaa gcgctttgat ctccgtctcc
     5461 ttcgcgccgt caaagaccgc cgttccgaac catgcgccct tctccatacc tgcgcagacg
     5521 cggatgagtt gctcgtcttc aagttccagc aattgccgga gtaccgcggt gttggcgaac
     5581 ttctccttca ccacttcgcg cagacgcgcc gggtcgcggt actggttgac cattgctgcc
     5641 acctgttggc cgacttcgtg cgcggcccag cccaagtgcg tttccagaat ctggccgacg
     5701 ttcatacgcg aaggcacgcc caacgggttt aggacaatct cgaccggagt gccatcaggc
     5761 agatacggca tatcttcctc gggcagcacg cgggaaatga cacccttgtt gccgtggcgt
     5821 ccggccatct tgtcgccgac tgaaagcttg cgcttcatgg cgatatagac cttcaccagc
     5881 ttgatcacac ccggagcaag ttcgtcgccc ttttgcagct tggcaatttt ctcgttcgtg
     5941 atcttgcgca ggacgtcaat ctgacgcgaa gtcatctcct caatctcgtc aatctgctca
     6001 ttcacgcgcg ggtctttgtc tgcgtagcga atgcgcttca gattgcgtgt cgagatcagc
     6061 tcgatagtgt cgcggtcaag aatcgtgccc ttggtgagca gtcgcttgtt cgtacgctcg
     6121 tcatgcaggt cggcaaggac ttctttgccg cccagaattg cttcaagacg cttcaaacgc
     6181 tcgtcggtga gaatgcggat ctcatcagcg aggttctttt cgagcttcgc cacctgctcg
     6241 gcttcaatca gcttggcgcg ctcgtccttt tcctgtccct tgcgggagaa gatgcggacg
     6301 tccaccaccg tgccttcgat gcccggaggg caggtcagcg acgcgtcgcg cacatcaccg
     6361 gccttttcgc caaagatggc ccgcaacagc ttctcttccg gagtaagctg cgtctcgcct
     6421 ttcggcgtga ctttgcctac cagaatgtcg ttgtgcttaa ccttggcacc gatgcggata
     6481 acaccgctct cgtccagatc acgcagcgcg tgttcgctga cgttgggaat gtcgcgggtg
     6541 atttcttccg gccccagctt cgtgtcgcgc gcttcaatct cgaactcttc aatgtggacc
     6601 gaggtgtagt agtcctcacg aacaagtttt tctgagatca ggatcgcgtc ctcgaagttg
     6661 tatccgcgcc agggcatgaa ggccaccagc acattgcggc ccagaccaag ctcgccctgc
     6721 tccgtgcaag gtccgtcagc aattacctgc cctttcacca cgcggtcacc ctggcggaca
     6781 atcggcttct ggttgatgca ggtgttctgg ttggagcgct tgaacttgat gagctggtag
     6841 atgtccgaac caacctcacg cgacaactgc gtcggatggt gctcgccttc cacgcgcacg
     6901 ataatgcgct cggaatccac cgagtccaca ataccgttac ggcgggcaag aacgacagcg
     6961 ccagagtcgc gcgccgtgac gccttccatc cccgtgccca caagcggagc ttcagaaacc
     7021 agcagtggca ctgactgtcg ctgcatgttg gcgcccatca gagcgcggtt ggcgtcgtcg
     7081 tgctcaagga acggcaccag cgatgcggcc acggaaacaa gctgtttcgg gctgacgtcc
     7141 acatagtcca cttcactgcg gttcacgagg acaaagttgc cctgacggcg ggcgttcacc
     7201 agctcgtctt tgatattgcc ttcatcgtcc agatcaatat tcgcctgagc aatcgtgtgg
     7261 cggtcttctt cccacgccga cagatagaag gagaatggct cgtactcaat cgtccgcttc
     7321 ttgtccttct taagctggtt gttcagcttg atggcttcct gcttctccaa gtggtcacca
     7381 acgcgcaggc cgctttcgcc tgcattggtg atctggacat agtcgagcac acggccatct
     7441 ttcacgcgcc ggtatggcga ctcgataaag ccgtactcgt taatgcgcgc aaagcacgag
     7501 agcgagctga taagaccgat gttcggacct tccggcgtct caatcgggca gatacgtcca
     7561 tagtgcgtcg ggtgaacgtc gcgcacttca aatcccgcac gttcacgcga caaaccaccc
     7621 ggacccagtg ccgaaagacg gcgcttgtgc gtgatttcag acagcgggtt cgtctggtcc
     7681 atgaactgcg agagctgcga tgagccaaag aactcacgga tggccgccat caccggcttt
     7741 gcattgatga ggtcgtgcgg catggccgtg gacatctcct gatacacgct catcttttct
     7801 ttaatggcgc gctccatgcg gaccagaccg atgcggaact ggttctccat caactcaccc
     7861 acggcacgca cgcggcgatt gccgagatgg tcaatgtcgt ccacaatgcc gatgttcttg
     7921 cgcagcttca gcagataccg gatcgtcgcg taaaagtcat ccggggtcag cgtgcggcgg
     7981 tcgagaggcg tcgcgtcctg gttctcatac agcttgatgt tgaacttcag gcggccgaca
     8041 cgcgagaaat catacttgcg cggatcgaag aacatgccct cgaacagcgc cgtcgccgtg
     8101 tcgagcgtcg gcggatcgcc cggacggagc ttgcggtaga tttcaatcag cgcttcttcc
     8161 ggcttgcgga ccgagtcgcg gcgcagtgta ttggtgatga cgttgcccac gtcatcccgc
     8221 tcagggaaaa agacctcaat cgtgctaacg ccactctgca taatcttgtg cagtttgtca
     8281 gcggtcagtt cctgattcgc ctcaaggaag acctcgcccg tggtcgtgtc aatcacgtct
     8341 gcggcagtga ctgcgccatc cagttcggcc gtctcgattt cgacttccga aaccttgtgc
     8401 tgacgcagtg ccttcagaat ggaaggcgta atcttgcggc cggagtgcgc aatctcctcg
     8461 cctttggcga caatggcatg cgcgggcttt gcacccagca ggtgggtccc cttctccgtg
     8521 tcctgctcaa ccgcccagaa cagcttgttg tcgcggacag aaatcttgtc caccgtgtag
     8581 aaggtcttca gaatctcttc atccgacttc agaccaagag cgcgcaggaa aatcgtgccc
     8641 aggaacttgc gtttgcggtc aatgcggaca tagagcgtgt tcttctggtc gtactcaaat
     8701 tcaacccacg atccacggta cgggatgatc ttgccgagga aatacgtgcg gttgtttgcc
     8761 gtctcaaaga agacgcccgg cgaacggtgc aattgcgaaa caatgacgcg ctcagtgccg
     8821 ttcacaatga acgtgccgtt ctgcgtcatc agcggaatat cgccaaagaa aacttcctgt
     8881 tccttgatgt cgcggatcgt cttcgcgccc gtttctgcgt ccttgtcata aatcgtcaga
     8941 cgcacggtga ccttcagcgg ggccgaatac gtcatgccac gctcttcgca ttcagactgg
     9001 tcatacttca gttgcaaacc gaccgggtca ccgcacttgt tgcagaagtc cggggtgttc
     9061 ttgttgtagg tgccgcactt gtggcaaagc acatcgcccg gatggaacgg atcggtgatc
     9121 accatcgcgc cgcaattgac gcaggtggta cgcaggtggt gtagcccctt caggtgtccg
     9181 cacttacact cccagttccc gatggagtaa tccacgaaat cgagttgcga gatgttgcgg
     9241 aagtcagcaa tggggaacac cgaagtaaag acagactgga gtccgttatc ttcacgctca
     9301 gacggcaatt tatccatctg caggaagcgc tcataggagc gcctctgcac ttcaatcagg
     9361 ttagggattt tgatggctgt tggaattttt gaaaaatcga gacggctgcg aatggcgcgg
     9421 ttctcgttcg gcatgcttca ctcctggggc ctgtttgcgc gagagattct gacatgctgt
     9481 ccaggccgcg cagcatgccc tgatgacgaa gtccgccgca gcgcctctcg cccgctgcgg
     9541 catctaaaaa cttctttcca tgcgtgcccg cgctccaaaa tggaacgcag ggcgaaatcc
     9601 cccggacttt ccacatctcc gggcttgcat ctgcaggcaa cctcgcgata caaacaaaac
     9661 gtcaaacaga ggtgcctagg ttatatctca ggcgggatag ttcgcagggc atagaaggaa
     9721 aagacaaagc ggatgaaaac tgcacaaacg cgaaaagccc gtaaagacaa gacgtcttac
     9781 gagcgccaca acccggattg tgcactttta aaatacagaa tgttcgcttc cgccgctctg
     9841 aaaccgtgta tgccgcccta cagctgatcc aaacctcgca cccgcaggcc cgccgctatc
     9901 tcccacagat acgtcggcac aaatatatgc cgaaaagccc tctatccacc ctccgcatgc
     9961 cagcaagccg cgcaattctc tgtcgcgcct ggacttaccc gcggaatcct tactgcatgc
    10021 cgtcagacac aaggaaaaac cagacggaat gcgcactttt catcatagcg catcccgttt
    10081 tggcatgcaa cttgccttgc aaattttttg atgcaaattc gccaattgcg gtgcaatttt
    10141 gcatcaaaaa tccggcccga caaaggcagg aacaggcaaa aattacttga tttcgatggt
    10201 agcgacgcct tcaaactttt tcttgatggc ctcggcatct tccttgctga cgttctcttt
    10261 cagcgtcttg ggagcgccat ccacaagatc cttggcttcc ttcagaccga gcgaagtcac
    10321 ttcgcgcacg gccttaatgg tgttgatctt gttcgcaccc gcatccttca ggatgacggt
    10381 gaactcggtc tgctcagccg ccgcaggggc cgccgcgccc gctccggcac ccgctccggc
    10441 tacgaccaca ggggccgctg ccgccgccga gacgccaagg cgctcttcaa gtttcttcac
    10501 cagggccgct gcgtccaaca ggctaagccc gacaatctgt tcctctaact gctgcaaatc
    10561 cgccattgat gttctccgta atacgaaatt tgtttaccac cccggagcgc ccgtgctccg
    10621 gttgccgcct gcccgccatg aagtttccga tgtgcccaga cccgcaccct tcacgctctg
    10681 ccttgcggcg aatctcttta gccttttagc cgccagaagc tagctcgctt ctgcaaactt
    10741 cttttgctcg accgcttgat tgatgaccac cgccaggtcg cgtcccgtcg cattgatgac
    10801 cgtcgccaga cgctgcgccg gagcattgat aaggaagagc agcttcgaga agatctcttc
    10861 cttgcccggc atggtcgcca actgtttaat ctcgtcaacc gtgatgacct taccatcaat
    10921 aatgccaagc ttgaatgtaa actcggcgtt ctccgccacc cagttcgaca gcgccttggc
    10981 caacgccacc gggtcaccgc tcgtgtacgc aaccgaggaa acgcccttca gcccctggag
    11041 cgccgcttca atctgcgttc cctgcgccgc gcgcgccgcc agcttgttct tgagaacacg
    11101 atagcgcccg cccgcagcgc ggaccgtctt acgcaattca aaatcctggg cgaccgtcag
    11161 tttggcaaat gtgccgataa tcgcgctggt cgaaccctgc agctcccgcg caagctgcga
    11221 aaccttttct gccttcttcg ctttggtcaa tgccataata agcctctagc tgttagctac
    11281 tagctcttag ctaaatccct gtttcaaatc ctctaaaagc cagctaacgg ctagaagcta
    11341 gctggctaaa agccgcctac gcctttgcga cctggtcggc aatcgcacca tcaagcggaa
    11401 tgcccgggcc catcgtcgag cttagcgtga ttcccttaat gtatttccct ttggcagccg
    11461 aaggctttgc gcggacaacg ctcgtgatga ccgtcaacgc attctcgacc agcttttccg
    11521 ccgggaagga aatcttgccc accggaacat ggaccaatgc cgtcttgtcg gtacgaaact
    11581 caaccttacc agccttgatt tccttaattg cagcagcgac atcggtggtc actgtacccg
    11641 tcttcgggtt gggcatcagg ccgcggggcc cgagcacctt accgaggcgt ccaaccgact
    11701 tcatcatgtc gggagtagca atcagagcat cgaagtccgt ccagctttcc ttctggatct
    11761 tctcaaccag ttcctcgcca ccgacaatgt cggccccggc agcctctgcc tcgcgcagct
    11821 tctcgccgga ggtaatgact gcgaccttct tggtcttgcc cagcccatgc ggcagcacca
    11881 ccgtgccgcg gaccatctgg tcggcatgct tggggtctac gcccagacga agcgttagat
    11941 ccaccgtttc atcaaacttt gcaaacttga ctttctgcag gagcggaacc gcttcctgaa
    12001 gggcgtaggg acgcttttcg acagcagcgc gcgccttcgc cgtattctta ctgactcttc
    12061 tggccatgta tcctcgtctc ccaccgcaca gtcgttcccg ctgctggccg tggtgctatt
    12121 gactcggccc gagctggcgc tcaggacgca tttctaagta tgccgtaaaa ggaggcagaa
    12181 agcaactatg ccccaaaaag aaagggcacc acggcatagt ggtgccctcc caaaatgaac
    12241 tgcatgagat cagaagacga acttgcctcc aatctgaagc gtacgaggag tggttgcgtt
    12301 ctccgcattg ctcataaatg gattgaagcc cgtcggcgag tattgctcca ctgacacatt
    12361 cggagtacc
//
LOCUS       DASRBV010000233         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_369280, whole genome shotgun sequence.
ACCESSION   DASRBV010000233 DASRBV010000000
VERSION     DASRBV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369280"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFA02_08755"
     CDS             <1..1245
                     /locus_tag="VFA02_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09983.1"
                     /translation="GDIASFILEYQQAVRLRPSDPGAHLDLANAYMSAKKLKDALDEY
                     KVAAQLAPKSPEVHYRYAMALKAAGKTSEEQAELQKAVQLRPDYAEALEALGDLLVAQ
                     KNYQLAIPTYRDAVRMLPNDLDLRMRLARALEQNGDPQGAVQVLQEAADLDSTSVQYE
                     LQLADALVAKKDFQSAARVYRQALAQKPSDPQLSMRLGNALLQMGDTKGAAEAFRQAL
                     KDQPGSPQAHLELAGMLEQTGDYESAAEEAKTALKTIPNSAKGHYLLGVALIGQKRTD
                     DGIKELREALRDNADYSEARLALANALRQDGQLYEAIGHYEQYLRDNPSDAGVHLILA
                     DALNERKNYAEAIEQCEEAVRLMPDNAQAHYDLAVNLEKLGKNADARREYETYLKLDP
                     NAPNAEAVRTLMRKLAKLESRG"
     gene            complement(1322..2500)
                     /locus_tag="VFA02_08760"
     CDS             complement(1322..2500)
                     /locus_tag="VFA02_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU09984.1"
                     /translation="MTDPASVSAHSIPPLTMLSEEEQMFRTAAREFAENEIGPHVEAM
                     DREGVFRQDLIEKFFQQGFMGIGVPENYGGSGGSFFMAVLAIEEFSRVDASAGVVIDV
                     QNTLVSNAILRWGTEEQKKAFLPRLVRDTVGSYALSEAGSGSDAFALETRATEDADHY
                     VLNGRKLWITNAREAGIFIIFATVNRDLGYKGITAFLADRSMPGLTVGKKEDKLGIRA
                     SSTCELVLEDCRIPRQNVLGEVGKGYRVAIETLNEGRIGIAAQMVGVARGAFDHALQY
                     AQERKQFGKPICEFQAIQFQLARLATQIEAARLLTYNAARLKDAGLPFLREAAMAKYF
                     CSNVAERVASESVEIYGGYGFTKDYPAEKYLRDSKIGKIYEGTSFMQLQTIAKSLLGR
                     "
     gene            complement(2657..>3124)
                     /locus_tag="VFA02_08765"
     CDS             complement(2657..>3124)
                     /locus_tag="VFA02_08765"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HZU09985.1"
                     /translation="QGDFTLDSGAPAAWMYGQHDIRFLSPNSTGIFTLGLFDNGYTRV
                     LDDNGDLCGTTGQPACYSRVPFFQVDEAGRTAHVLWEDSPLDYSEAVGSMDLLPNGDV
                     EFDEGFLRANPFNSRVMEVTKEPTPQPVWELDVTGQIGYRIRRIPSLYPGVQW"
BASE COUNT          673 a          898 c          928 g          625 t
ORIGIN      
        1 ggcgacatcg ccagttttat tctggaatat cagcaggctg tacggctccg cccgagcgat
       61 ccaggcgccc acctggacct ggcaaatgcc tatatgtctg cgaagaaatt gaaggatgct
      121 ttggacgaat acaaggtcgc agcgcagctt gcgccaaaaa gtccggaagt ccattatcgc
      181 tacgctatgg ctctgaaggc ggcaggaaaa accagcgagg agcaggctga gttgcagaaa
      241 gcggtccagt tgcggccgga ctatgccgag gcgcttgaag cgctgggaga ccttctggtg
      301 gcccagaaga attaccaatt ggctatcccg acgtaccgcg acgccgtgcg catgctgccg
      361 aacgatctgg acctcaggat gcgactggcc cgcgcgctgg agcagaatgg cgatcctcag
      421 ggagcggtcc aagtgctcca ggaggccgca gaccttgact ccacttccgt gcagtacgaa
      481 ttgcagcttg ccgatgcgct ggttgccaag aaagattttc agtcggcggc cagggtttac
      541 cggcaggcgc tggcgcagaa gccttcagac cctcagcttt cgatgcgttt ggggaacgcg
      601 ctgctgcaaa tgggtgacac gaaaggcgct gcagaggcgt tccgccaggc cctcaaggac
      661 cagcccggct cacctcaggc gcacctggag ctggcgggaa tgctggagca gaccggcgat
      721 tacgaaagtg cagcagaaga agcgaagacg gccctcaaga caattcccaa ttccgccaag
      781 ggacattatc tgttgggcgt tgcgctgatc ggccagaaga gaactgatga cggcatcaag
      841 gaattgcgtg aagcgctgcg cgacaacgcc gattacagcg aggcgaggct tgcgttggcg
      901 aatgctcttc ggcaggatgg gcaactctat gaggcgatcg gccattacga gcagtacttg
      961 cgcgataatc cgtcggatgc cggggtgcac ctgatcctgg ccgacgcgct gaacgaacgc
     1021 aagaattacg ccgaggccat cgagcagtgt gaggaagctg tgcggttgat gcctgacaat
     1081 gcgcaggcgc attacgacct ggccgtcaat ctggaaaaat tgggcaagaa cgcggatgcg
     1141 cgccgggagt acgaaacgta tctgaagctc gatcctaacg ctccgaatgc agaagccgtt
     1201 cgaacattga tgagaaagtt ggcgaagctc gaaagccggg gatgattgtt gatactgcgg
     1261 cgcagcgccc gcttctcgct aaaagaggcg accccgttga gaagcgccaa ttgtcgtcaa
     1321 cttaacgccc cagaagagat ttggctatgg tctgaagctg catgaaggat gtcccctcat
     1381 agatctttcc gattttcgaa tcacgcaggt atttctctgc agggtaatct ttggtgaatc
     1441 cgtagcctcc gtaaatttcg acggattccg aggccacgcg ctccgccaca ttggagcaga
     1501 aatacttggc catggcggct tccctgagaa agggcaagcc ggcatccttg agccgagctg
     1561 cgttgtaagt cagcaggcgc gcagcttcaa tctgagtggc cagccgcgca agctggaact
     1621 ggatcgcctg gaactcgcag atgggtttcc cgaactgctt ccgttcttgc gcatattgca
     1681 gggcgtgatc gaacgcgccc cgggccacgc ccaccatctg cgcggcaatt ccgatgcgac
     1741 cttcgttcag agtctcgatc gccactctgt aacctttgcc aacctctccc agaacgtttt
     1801 gccggggaat acggcagtct tcgagaacca gttcgcaggt ggatgaagca cggatgccga
     1861 gcttatcttc cttttttcca accgtgagtc ccggcattga gcggtccgcg aggaatgccg
     1921 tgatcccttt gtatcccagg tcgcgattga ccgtagcaaa gatgatgaat attccggctt
     1981 ccctggcgtt ggtgatccag agctttcgtc cgttcagcac atagtggtcg gcatcttcag
     2041 tagcgcgcgt ttccagcgca aaggcatcgc tgccggagcc tgcttctgac aaggcgtagg
     2101 agccgactgt atctcgcacg aggcgcggca gaaatgcctt cttctgctct tcggtgcccc
     2161 agcgcaggat ggcgttcgaa accagcgtgt tctgaacgtc aatcaccact cccgcggaag
     2221 cgtcaacgcg cgaaaactcc tcaatggcga gcacggccat gaagaatgat cccccgctgc
     2281 cgccgtagtt ttccggcacg ccgattccca tgaatccctg ctggaagaac ttctcgatca
     2341 ggtcctgacg gaagacgccc tcgcggtcca tggcctcgac gtgcggaccg atttcgtttt
     2401 cggcaaactc gcgcgccgcc gtccggaaca tctgctcttc ttcgctgagc atggttagcg
     2461 gcggaatgga gtgcgctgac acggaagccg gatcagtcat gaaatttgac ctcgcgcaaa
     2521 cgccctcatc ttatcaccgt cgcaacgcag tgcaaacgtc gcgggatcgt cgatgagagc
     2581 ctgttcattc agccgccgaa aggaagcgga gcatgctgcg gcggaaatgt gcaacagaca
     2641 gcgccctgcc cgggtgctac cactgcacgc cgggatagag gctgggtatg cgccggatcc
     2701 ggtaaccgat ctgaccggtc acgtcgagtt cccagaccgg ttgcggtgtc ggctccttgg
     2761 tcacttccat tacgcgggag ttaaatggat ttgcacgcag gaagccttca tcgaactcca
     2821 cgtcgccgtt tggaagcaag tccatactcc ccactgcttc tgaatagtcc agaggcgaat
     2881 cctcccagag gacgtgcgcg gtgcgccccg cctcatccac ttgaaagaag ggtactctgc
     2941 tgtagcaggc cggctggccc gtggttccgc acaggtcacc attatcgtcg aggacccgcg
     3001 tgtaaccgtt gtcgaagagt cccagagtga aaatgcccgt gctgtttggg ctcaggaacc
     3061 gaatatcgtg ttgcccgtac atccaggcgg cgggcgctcc cgaatccagt gtgaagtcgc
     3121 cctg
//
LOCUS       DASRBV010000234        18777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_164724, whole genome shotgun sequence.
ACCESSION   DASRBV010000234 DASRBV010000000
VERSION     DASRBV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 18777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18777
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164724"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFA02_08770"
     CDS             complement(<1..779)
                     /locus_tag="VFA02_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09986.1"
                     /translation="MFCRWTAWCALVLLSGACFASMTLENPQQTLRVEVRGTGVNATE
                     TISIKTGTGWVPALSATGPSVLVKTPDGAKRCAMMRASSVEHGLLLTGDCGIGTLEQR
                     IMLTPEEDILAVSTRLTLRRGVHVHSVEDRYDFMPERRSTDTPKSGPLDFIWSQNIKS
                     EAEDFIPAYGFKSPAVMLQQGQVFTALLPHLSNRKAEPLALDLDVTSEKRPWISYGAI
                     PAQPHGHSYFRRAPDGAPKILADTVAYSYSIVASRQPVKLG"
     gene            complement(779..1816)
                     /locus_tag="VFA02_08775"
     CDS             complement(779..1816)
                     /locus_tag="VFA02_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09987.1"
                     /translation="MNFYRPRLAAFALLLCFVPCALAADSLWVRPQKPGGPLIWGRRD
                     GIIFGLPSPGGLKGPRGLIRVGIIAPPDHTPKLLNYIAIEPVIQGPGSRFDRMAFSEL
                     EPSMLDPGERGKRLWVDPDMPASGNLETLHAGAAIVERLSVRIDVERFTANGAHVYVI
                     ASIDSDHPKELRLSVFKENDGPPLDELTVTATMGNYERLRLLWLKGRVEDSRKLFADY
                     QGNAFAEHENYPLDEMLRTGDGDAIVYCTSNEQSPATAPWNETAHWKYVLPKLTQYWR
                     VQAHDIQPDLRVRVNARRVYWASTAPVPGGIAFENFEVRQRYVPGQTFIFGISEKGPW
                     ELDTRLPKGDQ"
     gene            1971..3017
                     /locus_tag="VFA02_08780"
     CDS             1971..3017
                     /locus_tag="VFA02_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU09988.1"
                     /translation="MATIVEVAKRADVSIATVSNVIRGTRRVSQKLTERVQAAIRELN
                     YSPNEIARSLKVRQTRMLGMVLPDITNPFFPEIIRGAEDTAFERGYFLVTANTDEQIG
                     RERRIVSALRSYRVDGVLLASASGKDTAHLKSTMDAGVALVCLDRTVPGIKTDAVLLD
                     NVRGARECVRHLIQKGHTRIAIITGALNLQTGRERLRGYEDALTDAGLSIDPKLVFEG
                     DFRYESGHRLGKQIMKQTNRPTAIFVCNGVMAIGVLKAFEEIGAECPRDVALATFDDM
                     KLDSPFHPHLTTVVQPSYEMGARAATILMDRIEGKLKKAPFIVRVSPTLILRESTEAY
                     LHPAPPGKDHRNRR"
     gene            3177..6902
                     /locus_tag="VFA02_08785"
     CDS             3177..6902
                     /locus_tag="VFA02_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU09989.1"
                     /translation="MKTLKLWLAVLPLILFFVAPSALVGQADRGAIKGEVQDTTHAAV
                     PNATLTLKNEATGVAVTTASGASGQYSFLDLQPGVYDLTAEAPGFGTVVRQHIEVGVG
                     NTVALTVTMQPGQVQQTVTVAASAATVDTQTSDVGTTITPREIKDLPVSLSGDMRNPL
                     NFVVLAPGVYGSTPGPTPDYRLHISGSVSYANEVYIDGIPVMNTNQSGDIGSNHPPID
                     AISQFKLINNTQTAQYGFSSGIVSFAFNSGTDDFHGSLFEYLQNDALNAAGYVTNRLG
                     LKKAPLKQNEFGGTLGGPVWIPKLYDGRDKTFFFVDFTEFKYRPSANNATLTTLPTPF
                     RNGDFSPALGPQLTVNGQPVFDPEGRPVYTGAIYNPFSVHTVVGPDGKSYQVRDPFPG
                     NIIPSGFTGLSSVSQTVAKSFPDANNNAIFNNFVRRQSSKIDEHRMVIKLDEHISDKH
                     SLSGSYFFGGFSNSNNGGLNELDAVTNTAPTKQIRISYNYTHSPTLLNNVNLGYIRDT
                     NFSGPLVPGPGLAALGIQGLPPLAKDSSYPGISIGTVQNSIGGVGASKVAENRYIVND
                     NLTMVRGQHTFTLGGEVRWLQRNEGGLPGGSFTFEPTQSALNGTGFINGNQPVTIPVG
                     TGAPAASFLFGGMDFSRFDYPVESAYRWWQTGVFFQDDWRATPNLTLNLGLRYDLQIP
                     RTDTHGDVSTMDPTLPNPAAGGIPGAFTYYGHGPGRNGHTRIGEIDWKGLQPRIGFAY
                     SPGPSHTTAIRGGFALTRPLGNDNLENGIGGGLYNTGFAGLATVNRPQDYVGSPAYYW
                     NNPYPQSAVSPPGSHLDPGVLVGNDNPPMIHPRSGIPPTQMYWSMQVQQQVTSSMVAT
                     FGYVGMHTYHLGVWSKPNEVNPTEARQKYGPAAQAVGLPLNEFLALPINDPRVSAAGI
                     LPPWPGFAATFGPGATASQALRPWPQYGDVDNPLNPIGSVSYNGLQTSLQKRFTQGLT
                     FLLSYTFSKTIGDVDSNNGALAGAENAIYAGSFYQDYYNNKAERAVTSSDIPHVVSLS
                     YTYELPFGKGKPFLGHGGAVNTVVGGWSVSGIHHYQSGRPIHIEYDAFGASNPFFAAG
                     DGFSFRPNIVPHQPFKNPAYRRHCSGPTGGTGREPCQFYINPAAFSLPAPGEFGNAPN
                     LISALRMPAFINEDLSISKRTTLFEHLNLQIQANFFNAFNRTIFSSGGNAQTFIINGA
                     PPDLSPASLANSNTIFGIMTAQQNAPRIIQFGMRLEF"
     gene            6912..8288
                     /locus_tag="VFA02_08790"
     CDS             6912..8288
                     /locus_tag="VFA02_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09990.1"
                     /translation="MFAAILAVSVGMGTPLLAQTSAGEQLAQHAQKAQEAEQRNDFAS
                     AVKEYEFMAAALPRNAEIQSNLGVALYFNHGLRRAITVLHKAEALNAKLLAPHLFSGL
                     AYYHLSNPGAAAPELEEAVRISPSDTLAHTWLGYTYEALSLHEKALAQFQSAERLQPR
                     NVDIWYAIGQTCLEIGKEKTKELLRSAPNSARVWQLAGEQFELKGDSGKAKAAFAEAH
                     RLRPGFTGCPGNGSVEDSLYCKAHDAETSAQVAFQRVLQEAPNSARAHQIMADSYFAR
                     QQLDNATSEYRLVLQADPRLPNIHLQVGRCLMLRGQFDAALHEYEEEEKLQPESAQVQ
                     ADMGRVLLAMDRNREAAATLRKAVALDRPPVEAYFLLGKAELRNHNENEAVRFLQHYV
                     TAKPDDSAGYYLLSMAYRATGDKARMREAIELYKKTSQDTKERNLAQMELDRRNNTET
                     AEEGNPQS"
     gene            8330..9934
                     /locus_tag="VFA02_08795"
     CDS             8330..9934
                     /locus_tag="VFA02_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HZU09991.1"
                     /translation="MKEAVLITSGDLRESANKVCWPAQEKLERKLTECFQKEGVTLRR
                     AFLVDKERGHGFISSQRMGMDIFANVDPHANLVFATAAWQYTHHVLPGMRGHQGPILT
                     VANWSGQWPGLVGLLNLNGSLVKAGKSFSTLWSEHFNDAYFLNKLHEWVRTGKVTHDL
                     SHVRDLDPKNLPAEQAKLGKKLAEELLKKKIILGVFDEGCMGMYNAIIDDELLNPSGF
                     FKERLSQSALVAKMRTVSDKEAAAVRKWLDHRGVKFETGKDTQSELTDEQIHEQCKMY
                     IAATRMAYEFGCDAIGIQYQQGLKDMVPASDLVEGLLNNAERPPVFDEYGRELYAGGP
                     LPHFNEVDEGAGVDAVVTNRCWTALGLDPSTTLHDVRWGGPCPVNGKTEFVWVLQISG
                     AAPANHFVNGYAGATSSRQPPMYFPLGGGTLKGIGKPGKIVWSRVFVEGGSLHVDMGL
                     GSVVLLPEEETKRRWQQTTPQWPLVSATFDGVSRDAFMARHRANHVSIAYAGSAEEAA
                     KALAVKAAMFDALGIRVHLCGAINIG"
     gene            9974..11314
                     /locus_tag="VFA02_08800"
     CDS             9974..11314
                     /locus_tag="VFA02_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HZU09992.1"
                     /translation="MSISRHVVKSTVVGALGGLLFGFDTAVISGTTQALTAQFRLSPG
                     MLGFTVASALWGTVVGAITAGIPGQKYGRRDSLRVMALFYVVSALGCALAWNIESLVV
                     FRFLCGLAIGGSSVLGPMYIAELSPPKWRGRLVGFFQVNIVVGILLAYMSNYAIAAMQ
                     LGAAEWRWQLGVSGFPALLFLIMLFFIPRSPRWLTTQSRLDEAYSVLVLTGVDDPQRE
                     LDEIRDSLHLESRGDEDSLWSRKYKLPVFLALTVGAFSQLTGINAILYYLNDIFALAG
                     ATKISGSLQAVAVGATNLVATLIAMSVIDKFGRKKLLLGGTVGLAICLTVISVLFLSH
                     SHLHLLVWLLMIYIALFAVSHGAVVWVYISEVFPNRVRAKGQSLGSSSHWVSNAIISL
                     IFPIFAKSSGAVPFLFFAAMMVLDFFIVLFIYPETAGVSLERMQERLESTPVTR"
     gene            11321..12442
                     /locus_tag="VFA02_08805"
     CDS             11321..12442
                     /locus_tag="VFA02_08805"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HZU09993.1"
                     /translation="MRLITGFLLGVVAASACAQVQNWQPDLTRQQTYTLHRESSTDPT
                     GGNADFRVVAPGATQTVLDVDGPGIISHIWFTIADSEPYHLKRIVLRMYWDGETSPSV
                     EAPIGDFFGLGLGTYHNWQSQMLSVGSDKALNCFFPMPFQHHARITVTNEGTHQINAL
                     YYNIDYRKLSASLPSDTVYFHAQYRQAQPNHGTVSGWQDNGDREVNDKKNLDGADNYI
                     WMEAKGHGQYVGVTMSVLQNQDFWWGEGDDMFFIDGAAKPQIVGTGSEDYFLGAWDFG
                     GHPFSYQLYGAPVVGEELAGSRSSVYRFHLDSPIPFQKSFKATIEHGHANARSDNYYS
                     VAYWYQSEPHEAFPPLPPVEQRIPALQFVGGPGNSTAKK"
     gene            complement(12496..12954)
                     /locus_tag="VFA02_08810"
     CDS             complement(12496..12954)
                     /locus_tag="VFA02_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZU09994.1"
                     /translation="MPKRSAGILMYRRQANGTEVFLVHPGGPFWAKKDLGAWSVPKGE
                     YLPGEDPLTVARREFQEETGLEVSGDFLPLGEEKQPAGKLVSVWAVQGDCSPAGLRSN
                     TFQMEWPPRSGRTVEFPEVDRWEWFSLNEARKRILPGQKVFLEKLAETLR"
     gene            13011..14114
                     /locus_tag="VFA02_08815"
     CDS             13011..14114
                     /locus_tag="VFA02_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU09995.1"
                     /translation="MGSTPVLQDELQGSVFVLIQFDVCEEIRLDRLKQIFSAQTVQRP
                     ALKHALPSYIRYERPPVVEATGPLVLESGERLHGQIKYYDYGVVSVILELPFAGNWEK
                     LVQLSSRWVWDVDFSGQVLRIVKERLHQAADALAKPYEEWLSEDYFIFHVAELAGPLT
                     AAQVIQLRGKEISQIVRGDATELSDSERQEVLQSGISYGIKDLAVIGWNAAFLYDTQS
                     GADTAIQLLEYANSQLLEFRHYDELLTNELESVYNSLEKGTGFFARWRLARSATKLNT
                     VLLEVTELTEHADNAIKFLSDMFSARLYKVAAAKVGVPDYKNLVTNKVQTARELYHFM
                     VDQFNQGRAFFLELTVVIILIIELLYLFRGHPF"
     gene            complement(14111..17617)
                     /locus_tag="VFA02_08820"
     CDS             complement(14111..17617)
                     /locus_tag="VFA02_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZU09996.1"
                     /translation="MSKLQLVPPDQAQRDEALNTAISCIVQAPAGSGKTELLTQRFLK
                     LLAEVDEPEQVLAITFTRAATAEMRSRILQQLEKARRGKYDPALETARKALAQDEARG
                     WQLLEQPQRLNIQTIDSLCMRIAHEQPLLARMGGHLQPQEDARALYDEAARRTLANLG
                     STKDEIHSALMHLLALRDNNLPDAQRLIAEMLAKRDQWLHVFPLSNEISEEEWAEVRR
                     KLEAPFRSAVRQALKRAHQHIAAEPLLADHLLRLSNYASANVNNLDLAAIAHIRALPG
                     TSEQFLDQWNCICRLLLNNEDEWRKTVTVREGFPSTPNHKNSTEQQYKEQMIGLLKKI
                     SGITGLREALCAIRKLPPLHYDEGQWHTLRQLFIVLRYAVAELRVLFAERNIIDFVEL
                     GIAALQVLENPTENVLRFGEQIRHLLVDEFQDTSRRQIRLLEMLLRAWGDNGRRTCFL
                     VGDPMQSIYMFRQAEVELFEQVQRHGIEEHSFRPISLSANFRSHAGLTEPLNAWFANI
                     FANHHTPGSAAVPFSRSEAVVPALPGQSVHLHPHFVPKGDKAETERAAKQEANEVIQV
                     IQQHLPRIRKAETSPEDTFTVAVLARARQHLPLIARELREKKIPYRAVEIELLGERQE
                     IRDLLSLTRALLHPMDRIAWLSVLRAPWCGLTLQDLHTLCGEDDRRFARVPVSRLVEE
                     RRVLLSKDGQQRLERTFAVLERALETRYQQGLSLSTWIERTWHSLGGPLCLDATQQEN
                     ARVFFSMLDSISADGIACLGDELDQQLERLFAQPDPSVSDRAGVQLMTIHKAKGLGFD
                     VVIVPGLERTTRSDNKPLIVMLQRANTANNNEDELLIAPIDSRGNGTHPLYRWVRKLR
                     DLREEEELKRVFYVACTRARSELHLSGTATVTNDDKLSSAANTLLCAAWPALETEFAS
                     AYEEKKRQNVISFPAPQPGVVDIAAEAAPSHPPLMLHRLPMDVVLPQRGANIKVPGPV
                     SSVEDYELFERPEGGRMQRLTGSIIHTLLERLSRMQGVSLAEIAVWIERHASAMLRAA
                     ALPAEEASHVLNEVVASVSAAIEDPQGQWLLAPHPDAQSEASWTGWIEGALRTVRADR
                     VFRAGSGPLSAGRDFIWVVDYKTIPYTGTDVARFLAAQREQYGPQLAMYGKALQKIHG
                     KDTRLRFALYFPRLKQLASWGD"
     gene            complement(17610..>18777)
                     /locus_tag="VFA02_08825"
     CDS             complement(17610..>18777)
                     /locus_tag="VFA02_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZU09997.1"
                     /translation="GLAHTVTQRIANSAPEFVFSYARQNKEGEQRPSPLLARIFGTNY
                     KTLSTHDLRAQLHGEEPALLAQELERLYQSSEIAHWPQDRAAGGASILKEQAACPFQA
                     FATRRLQAKPLNRAEWGLSAMDRGNLIHRILENIWSPKTPETFRMTGLEDLKQVMAAQ
                     RLDEVLRYHISHAFAGLMKENAGDAWMTSYLESEQQRLLVRLREWMQCEAERLPFEVE
                     RREEELKDVHVGGLKLNLRADRIDLLPDGSRLLIDYKTGDVSAADWQGERMKEPQLPL
                     YAIYGNVENVSGLLFARIRAGKTEFAGHVANAQAQFEAGLTAKSPLVNKPYNIKMREG
                     WQRALLGLAEEFLRGEASVDPKEGPKTCKYCPLPGLCRIAELDLATDGEEPDDE"
BASE COUNT         4185 a         5178 c         5168 g         4246 t
ORIGIN      
        1 tacccaagct ttaccggctg ccgcgaagcc acaatcgaat aggagtacgc aaccgtatcg
       61 gccaggatct ttggagcacc atccggagcg cgacggaaat aactgtgtcc atgcggctgc
      121 gccggaatgg ccccataaga aatccacggg cgtttctccg atgtcacgtc gaggtccagc
      181 gccagcggtt cggcctttcg gttgctcaga tgaggcagca atgccgtgaa gacctgcccc
      241 tgctgcaaca tgactgccgg agatttaaag ccatacgcag gaatgaagtc ctctgcttcg
      301 ctcttgatgt tctgtgacca gataaagtcc agcgggccgc tcttcggcgt gtccgtggaa
      361 cggcgttccg gcatgaagtc atagcggtct tccaccgaat gaacatgaac gccacgcctg
      421 agggtcaggc gagtagaaac agccagaatg tcctcttccg gagtaagcat gatgcgctgc
      481 tcgagcgtgc caatgccgca atcgcccgta agcagaagcc catgctcaac agacgatgcc
      541 cgcatcatcg cgcatcgctt cgcaccgtcc ggtgttttta ccagcacaga cggaccagta
      601 gcagacagcg ccgggaccca tcccgtgcct gtttttatgg aaatggtctc cgtcgcattc
      661 acgcccgttc ctcgcacctc tacgcgcagt gtctgctgcg ggttttcaag cgtcatcgaa
      721 gcaaagcagg caccagaaag cagcacaagc gcgcaccacg cagtccaccg gcaaaacatt
      781 actggtctcc tttgggcagc cgcgtgtcca gctcccacgg ccctttttca gaaataccga
      841 agataaatgt ctgcccggga acatagcgct ggcgtacttc gaagttctca aaagcaatgc
      901 caccgggcac cggcgccgta gatgcccagt acacacggcg cgcgtttacc cggacgcgca
      961 ggtcaggctg aatatcatgc gcctgcacgc gccagtactg cgtcagcttt ggcagaacat
     1021 atttccagtg ggccgtctca ttccacgggg ccgttgccgg agactgttca ttcgaagtgc
     1081 agtacacaat cgcatcgcca tcgcccgtgc gcagcatttc atccagagga tagttttcat
     1141 gttcggcaaa tgcattgccc tgataatcag caaagagctt acggctgtct tccacgcgtc
     1201 ctttcagcca gagcaggcgc agccgctcat agttgcccat ggttgcggtg acggtcagtt
     1261 cgtcgagtgg agggccgtca ttttccttaa atacggagag gcgcagttcc ttggggtggt
     1321 cgctgtcaat ggaagcaatg acgtagacat gcgctccgtt cgcggtgaag cgctccacgt
     1381 caatcctgac ggaaagccgc tccacaatag ccgctcctgc atgaagggtc tcgaggttgc
     1441 cggacgcagg catgtccggg tctacccaga gccgcttacc gcgctcgccc ggatcaagca
     1501 tgctcggctc aagctcgctg aaggccatgc ggtcaaagcg gcttcccggg ccttgaatga
     1561 cgggttcgat ggcgatatag ttcagcagct tcggggtatg gtcaggtggg gcaatgatac
     1621 cgacgcggat gagcccgcga gggcccttga gtcccccggg cgagggaagt ccgaagatga
     1681 tcccgtcccg tctcccccag atgagcgggc caccgggctt ttgtggccgc acccagagag
     1741 aatcggcggc aagggcgcat ggcacaaagc agaggagcag agcaaaggca gctaggcgcg
     1801 gtctgtaaaa attcatggcg tgtagtcttt cggcgcgaaa attcgtctac tccttttaat
     1861 cgtttaaata tatttctggc aagttataat ccgattgagt tacacggccc ggaactgaaa
     1921 aaattccttg ccaaaatcca cgaaagaaac gaatctactc tacacacccg atggccacaa
     1981 ttgtagaagt agcaaagcgg gcggatgttt cgattgcgac ggtctcaaac gtcattcggg
     2041 gcacgcggcg cgtcagccag aagctcacgg aacgggtgca ggccgccatt cgcgagctga
     2101 actattcgcc caatgagatt gcccgcagcc tgaaggtccg ccagacccgc atgctgggta
     2161 tggtcttgcc ggacatcacc aacccattct ttccggagat cattcgcggc gccgaagata
     2221 ccgccttcga gcgcgggtac ttcctggtca cagccaacac tgacgagcag attggccgcg
     2281 aaagacgcat cgtgtccgcc ctgaggtctt accgtgtaga cggtgtgctg ctcgcatccg
     2341 ccagcggcaa agacaccgcg cacctgaaga gcacgatgga tgccggcgtc gcgctggttt
     2401 gtctcgaccg caccgtcccc ggcattaaga ccgatgcggt gctcttggac aatgtacgcg
     2461 gagcacggga gtgcgtgcgt catctcatcc agaaggggca cacacgcatc gccatcatta
     2521 ccggggccct caatctgcaa accgggcgcg agcgcctgcg cggctacgaa gatgctttaa
     2581 cggacgccgg tctttccata gatccgaagc tggtttttga aggtgatttt cgctatgaat
     2641 ccggtcaccg gctgggaaag cagatcatga agcagacaaa ccggcccacg gccatctttg
     2701 tctgcaacgg tgtcatggcc atcggcgttt tgaaagcatt tgaagagatc ggcgcggaat
     2761 gcccaagaga cgtagcgctg gcaacttttg atgacatgaa gctggacagc ccgtttcatc
     2821 cccatctcac tacggttgtg cagcccagtt atgaaatggg cgcgcgggcg gcgacgattc
     2881 tgatggaccg gattgaaggc aagctgaaga aagcgccttt catcgtccgc gtttcgccca
     2941 cattgattct gcgcgaatct accgaggcat acctgcatcc tgccccaccg gggaaagacc
     3001 accgtaaccg ccggtgaggc caatgttttc acaccggaaa ggcgggaaat tcagcaaagg
     3061 aaaatttccc atcaaaaaat cttgacagtg tttttgtggc tgtgctctga taatccagaa
     3121 aatttaaagg tttaaaaata taaacgatta aatctgtaca ttatcgagag gcccccatga
     3181 aaacactgaa actctggctg gcagtcctcc ccctcattct gttctttgtt gctccttccg
     3241 cccttgtggg gcaggcagac cgcggcgcga ttaaaggtga ggtgcaggac acaacgcacg
     3301 cggccgtacc caatgccact ctgacactga aaaacgaggc cacgggagtc gctgtaacta
     3361 ctgcatccgg tgcatccggg caatacagct ttcttgatct gcagcccggc gtttacgacc
     3421 tgacggccga ggcccccggc tttggaacgg tcgtgcggca gcacattgaa gtcggtgtgg
     3481 gcaacacggt tgctctcacc gtcaccatgc agcccggaca ggtgcaacag acggtaacgg
     3541 tcgccgcaag tgctgcgacc gtggatacgc agacctccga cgtcggcacc acgattactc
     3601 cgcgggaaat caaagaccta cccgtatcgc tgtctgggga catgaggaac ccgctgaatt
     3661 ttgttgttct ggcgcccggc gtatacgggt ccacgccggg tccgacgcct gactaccgcc
     3721 tgcacatcag cggctcggta agttatgcaa atgaagtcta cattgatggc attccggtga
     3781 tgaataccaa tcaatcggga gacattggtt cgaaccatcc tcccattgac gccatcagcc
     3841 agttcaagct catcaataac acgcagaccg cgcaatatgg attttccagc ggcattgttt
     3901 catttgcgtt taattcgggc accgatgact ttcacgggag cctctttgag tatctgcaga
     3961 acgacgctct gaatgcagct gggtatgtaa caaaccggct tggcctgaag aaggcccctc
     4021 tcaagcagaa tgagtttggc ggcaccctcg gcgggccggt gtggataccg aagctgtatg
     4081 acggccgcga caagaccttc ttttttgtag acttcacgga atttaaatat cgcccgagcg
     4141 caaataacgc cacgctgaca acactgccca cgcccttccg caatggagat ttctccccag
     4201 cgcttggccc gcagctgacg gtcaatggcc agccggtctt tgatcctgaa gggcggcccg
     4261 tgtatacagg cgcgatttat aatcccttct ctgttcatac cgtagttggg ccggatggta
     4321 aaagctatca ggtgcgcgac ccatttcctg gaaacataat tcccagcgga ttcacggggc
     4381 taagctccgt ctcgcagaca gttgcaaaga gctttcccga tgcaaacaat aacgccatct
     4441 tcaacaattt tgttcgccgt caaagctcga aaattgacga gcaccggatg gtaatcaagc
     4501 tggacgaaca catttctgat aagcattcgc tttcaggaag ttatttcttt ggcggctttt
     4561 ccaacagtaa caatggcgga ttgaatgagc tggatgcggt cacaaatacc gcgccgacaa
     4621 agcagattcg catcagctac aactatacgc actcgccaac gctgctgaat aatgtgaacc
     4681 tcggctatat tcgcgataca aacttcagcg ggccgctcgt tcccggtcct ggcctggcag
     4741 cgctgggcat tcaaggactt cccccgctgg caaaagacag ttcatatccc ggcatcagca
     4801 tcggtacagt acagaacagt atcggcggcg ttggcgcatc gaaggttgcg gagaaccggt
     4861 acatcgtgaa tgacaatctg acgatggtcc gtgggcagca cactttcacc ctgggtggtg
     4921 aggtacggtg gctgcagcgg aatgaaggcg gattacctgg cggcagcttt acctttgagc
     4981 caacacaatc tgcgctcaac ggtactggat tcatcaatgg aaaccagccc gtcacgattc
     5041 cagtgggtac aggcgcgccc gcggccagct ttttgttcgg aggaatggac ttttcgcgct
     5101 ttgactatcc ggttgagtca gcataccgct ggtggcaaac aggcgtgttc tttcaggacg
     5161 actggagggc cactccaaac ctgacgctga atcttggatt gcgctacgac ctgcagatcc
     5221 cgcgaacgga cacgcatgga gatgtttcta ccatggaccc aacactgccg aaccctgcag
     5281 caggcgggat tcccggcgca ttcacctatt acggtcacgg accgggaaga aatggccaca
     5341 cacgcatcgg tgagattgac tggaagggcc ttcagccgcg catcgggttt gcctattcgc
     5401 cggggccatc gcacacgacg gccattcgtg gaggttttgc tctcacgcgt ccgctgggca
     5461 atgacaatct tgagaacggc attggcggcg gcctctacaa cacaggcttt gctggtctgg
     5521 cgaccgtgaa cagaccgcag gattatgttg gctcaccggc ctattactgg aacaatccct
     5581 atccgcagag cgctgtcagt ccgccgggat cgcatcttga tccgggagtg ctggtgggaa
     5641 atgataaccc accgatgatt catccgaggt cagggattcc gcctacgcag atgtactggt
     5701 ccatgcaggt acagcagcag gtaacgtcga gcatggtcgc aacctttggc tatgtcggca
     5761 tgcacaccta tcatctcggc gtctggtcga agccaaatga agtgaatccc acggaagcga
     5821 ggcagaaata tggcccggcc gcgcaggctg ttggattgcc gctgaacgag tttttggccc
     5881 tgcccatcaa cgatccacga gtttcagcag caggcattct tcctccatgg cccggatttg
     5941 ccgcgacatt cggaccaggt gcgacagcca gccaggcatt gcgtccctgg ccgcagtatg
     6001 gcgatgtaga caatccgctg aacccgatcg gaagcgtttc gtacaacggg ctgcaaacca
     6061 gcctgcaaaa gcgcttcacg cagggactca cttttctgct gtcatatacg ttctcgaaga
     6121 ccataggcga tgtggattcc aacaacggtg cattggccgg ggcggaaaat gcaatctatg
     6181 caggctcgtt ctatcaggac tactacaaca acaaagccga gcgcgcagtc accagctcag
     6241 atattccgca tgtagtttct ctgagttata cgtatgaact tccgttcggg aaaggaaagc
     6301 catttctggg ccatggcggc gcggtgaata cagtcgttgg tggctggtca gtttcgggca
     6361 ttcatcacta ccaaagcggt cgtccgattc acattgagta cgatgcattt ggcgcatcca
     6421 atccgttctt tgcagcgggt gacggattca gcttccggcc caatattgtg ccgcaccagc
     6481 catttaagaa ccccgcatac agacggcact gctctggccc aactggagga actggaagag
     6541 agccgtgcca gttctatatc aatccggccg cattttcgct gcctgctccg ggagagtttg
     6601 gcaatgcgcc gaatctgatt tcggcgctgc gcatgcccgc ttttatcaat gaggatctgt
     6661 ccatctcgaa gcggacgacc ctctttgagc acctgaatct gcaaatccag gcaaacttct
     6721 tcaatgcgtt caaccgcacg atcttctcct caggaggaaa cgcgcagacg ttcattatca
     6781 acggcgctcc gccggattta agtccggcat cgctcgccaa ctcgaataca attttcggca
     6841 tcatgacggc acaacaaaat gcgccgagaa ttattcagtt tggaatgaga ctggagttct
     6901 gaactgtggc catgtttgct gccatattgg ctgtttcagt gggcatgggc acgcctttgc
     6961 tggcgcagac ttctgctggt gagcagttag cgcagcacgc acagaaagcc caggaagccg
     7021 agcagcggaa cgattttgcg tctgctgtga aggagtacga atttatggct gctgcgctgc
     7081 ctcgcaatgc cgagatccaa agtaatctgg gggtcgcgtt gtatttcaac catgggttgc
     7141 ggcgcgcaat cacggtgttg cacaaagccg aagcgctgaa tgcaaaactg ctcgcgccgc
     7201 atttgttttc aggacttgcg tattatcacc tctccaaccc tggtgcggct gctccggaac
     7261 tggaagaggc cgttcgtatc agtccttctg acacgctcgc acatacatgg ttgggatata
     7321 cctatgaagc tttgtcactg catgagaagg cgcttgcgca gtttcagtca gcggagcgat
     7381 tgcagccccg gaatgtagat atctggtacg cgatcgggca gacctgtctc gaaatcggga
     7441 aagagaaaac aaaggaactg ctcaggtcgg ccccgaacag tgcgcgtgtc tggcagcttg
     7501 caggcgagca atttgaactg aagggagact ccggaaaagc aaaagcagca tttgctgaag
     7561 cccatcgttt gcgccccggg tttacaggtt gtccgggcaa tggcagtgtt gaagattcac
     7621 tttattgcaa ggcccacgat gctgagactt ctgcgcaggt ggcctttcaa cgcgtgctgc
     7681 aggaggcccc gaattcagcg cgcgcgcacc agatcatggc agattcgtat tttgcgcgcc
     7741 agcaacttga caatgcgaca tcagaatacc gtctggtgct tcaggcagac ccaaggctgc
     7801 ccaatattca tttacaggtt ggccgctgcc tgatgcttcg cggtcagttt gatgcggcgc
     7861 tgcatgaata tgaggaggaa gaaaaacttc agccggaatc tgcgcaagtg caagccgaca
     7921 tgggccgcgt tctgcttgct atggacagaa atcgagaggc cgcagcaaca ctcagaaagg
     7981 cagtggccct ggaccgtcct ccagttgaag catattttct gttggggaaa gcagaattac
     8041 gaaaccacaa tgaaaatgag gctgtgcggt ttcttcagca ttacgtgacg gcaaaaccgg
     8101 atgactccgc tggatattac ctgctgagca tggcttatcg tgccactgga gacaaagcgc
     8161 ggatgcgcga ggccatcgaa ctctataaaa agacatcgca ggatacaaaa gaacgcaacc
     8221 ttgcgcaaat ggaactggac cgcaggaaca atactgagac ggcggaagag ggcaatccgc
     8281 agtcatgatg ggattccacc aaaaccacaa aggaaactca atgccacaga tgaaagaagc
     8341 tgttctgatt accagcggag acctgcgcga atccgccaac aaagtttgct ggcccgcgca
     8401 ggaaaaactg gagcggaaac tgactgaatg ctttcaaaaa gaaggggtca cattgcgccg
     8461 cgcctttctt gtagataaag agcgcgggca cggattcatt tccagccagc gcatgggcat
     8521 ggatattttt gcgaatgtag acccacatgc aaatctcgtc tttgcaacag cagcatggca
     8581 gtatacgcac catgttctgc cgggaatgcg cggtcatcag gggccgattc tgactgtggc
     8641 gaactggtca ggacaatggc ccgggctggt gggcctgctc aatctgaatg gctcgctcgt
     8701 aaaggcaggg aagtccttca gcactctctg gagtgagcat ttcaacgatg cttatttcct
     8761 gaacaaactg cacgagtggg tcagaaccgg aaaagtaacg catgatctat cgcatgtgcg
     8821 cgatctggac ccgaaaaacc ttcctgccga gcaggcaaaa ctgggcaaaa agctcgcaga
     8881 agaactgttg aagaaaaaga tcattctcgg cgtcttcgat gaaggctgca tgggcatgta
     8941 caacgccatc atagatgatg agttgctgaa tccctcaggc tttttcaaag agcgcctgag
     9001 ccagtcggcg ctggtagcaa aaatgcgtac tgtctcagac aaagaagccg ccgctgtgcg
     9061 aaagtggctc gatcatcgcg gcgtaaagtt tgaaacaggc aaagatacac aatccgaatt
     9121 gactgacgaa caaatccacg agcaatgcaa gatgtacatt gctgctactc gcatggcgta
     9181 tgagttcggc tgcgatgcaa ttggcattca gtatcagcag gggctgaaag acatggtccc
     9241 ggcatcggat cttgtagaag ggcttttaaa caacgcagag cgtcctccgg tttttgacga
     9301 atacggacgc gagctttatg ccgggggccc gcttccacac ttcaatgaag tagacgaagg
     9361 tgctggcgtg gacgcggtcg tcacgaaccg ctgctggaca gcgcttgggc tggatccttc
     9421 gaccacgctg catgatgtcc gttggggcgg accatgtcct gtcaacggca aaactgaatt
     9481 tgtctgggtg ctgcaaattt ccggcgcagc gccggcaaac cacttcgtca acggatatgc
     9541 cggggcaacc agcagccgtc agcctcctat gtattttccg ctcggtggag gaactctgaa
     9601 gggaatcggc aagccgggaa aaattgtctg gagccgtgtg tttgtggaag gaggctcgct
     9661 gcatgtagat atgggcctgg gttccgtggt gttgctcccg gaagaggaaa caaagcgccg
     9721 ctggcagcag acaacaccac agtggccgct ggtgagcgca actttcgacg gtgtatcgag
     9781 agatgcgttt atggcccgtc atcgtgcgaa ccacgtcagt atcgcgtatg ctggcagcgc
     9841 ggaagaagcg gccaaggcac ttgcagtaaa ggccgcgatg tttgatgctc tcggaatccg
     9901 cgtgcatctt tgcggagcga tcaatattgg atgacccggg cagaaacgtc tctttagaaa
     9961 acagagtaat tgcatgagca tatcgcgtca cgttgttaaa agtactgtgg tgggtgcgct
    10021 gggcgggctt ctcttcggat ttgacacggc cgtcatctcc ggcaccacgc aggcacttac
    10081 tgcacagttt cgcctttctc ctggaatgct gggtttcacc gttgccagcg cgctctgggg
    10141 cacggtcgtt ggagcaatca cagcaggaat tcccgggcag aaatatggcc gccgcgacag
    10201 ccttcgtgtc atggcgctct tctatgtggt ttcggccctc ggttgcgcgt tggcgtggaa
    10261 tatcgaatcg cttgttgtct ttcgctttct ttgtggtctc gcgattggcg gatcttccgt
    10321 gttggggcct atgtatattg ctgaactctc tccaccgaaa tggcgcggaa ggctggtcgg
    10381 attttttcag gtcaatatcg ttgttggaat cctgttggcg tatatgtcca actatgcgat
    10441 tgccgcgatg caactgggcg cggcggaatg gcgctggcag cttggcgttt ccggttttcc
    10501 cgcgctgttg tttctcatta tgttgttctt tattccgcgc agtccacgct ggctcactac
    10561 ccagtcgcgg ctggacgagg catacagtgt gctggtgctg accggtgtgg acgacccgca
    10621 aagagagttg gacgaaatcc gggactcgct gcatcttgaa tcccggggag acgaagactc
    10681 attgtggtcc cggaagtaca aactgcccgt ctttcttgct ctgaccgttg gcgcgttcag
    10741 ccagcttacg ggcattaatg ccattcttta ttatctgaac gacatttttg cacttgccgg
    10801 cgcaacgaag atatccggca gcctgcaggc ggtggctgtc ggggcgacca acctggttgc
    10861 gacgctgatt gcaatgtctg tgattgacaa gtttgggcgc aaaaaactgc tgctgggcgg
    10921 gactgtcggg ctggccatct gccttactgt cattagtgta ctttttcttt cgcacagtca
    10981 tttgcacctg cttgtctggc tgctgatgat ctatatcgca ctttttgctg tgtcgcatgg
    11041 agcagtcgtc tgggtctaca tcagcgaagt gtttccaaat cgtgttcgcg caaaagggca
    11101 aagcctgggc agttcatcgc attgggtctc gaatgcgatc atctcgttga ttttccccat
    11161 cttcgcaaag tcgtccggag cagtgccgtt cctgttcttt gcggccatga tggtgctcga
    11221 tttctttatt gttctcttca tttatcccga gactgcgggc gtctctttag agagaatgca
    11281 ggagcgtctc gaatccactc cagtgacgag gtaagatacg atgcgtttga ttaccggctt
    11341 tcttcttggc gttgttgccg cttctgcctg tgcgcaggtg cagaactggc aaccagacct
    11401 tacgcggcaa cagacttata cgctgcaccg cgaatccagc accgacccaa ccggcggtaa
    11461 cgctgatttt cgcgtagtcg ccccaggcgc aacgcagact gttctcgacg tagatggacc
    11521 aggcataatt tcgcacatct ggttcaccat cgctgattct gagccgtatc acttaaaacg
    11581 cattgtgctg cgcatgtact gggacgggga aacatcgccc agcgtcgaag cgcccatcgg
    11641 agacttcttc ggcctcgggc ttggcacgta ccacaactgg cagtcgcaga tgctttcggt
    11701 aggcagcgac aaggcgctga actgcttttt cccgatgcct ttccaacacc acgcgcgcat
    11761 cacggtcacg aatgaaggca cacatcagat caacgccctc tactacaaca ttgattaccg
    11821 caagctctcg gccagcttgc cttcagatac agtgtacttc catgcgcagt accgtcaggc
    11881 gcagccgaac cacggcacag tgtcaggatg gcaggacaat ggcgacaggg aggtgaatga
    11941 caagaaaaac ctggatggcg cggacaacta catctggatg gaagctaaag gccacggcca
    12001 atacgtaggc gtaaccatgt ctgttctgca gaaccaggac ttctggtggg gcgagggcga
    12061 cgacatgttc tttattgatg gcgcagcgaa gccgcagatt gttggcacag gttcagaaga
    12121 ttatttcctg ggcgcatggg acttcggcgg acatcctttt tcttaccagc tctacggtgc
    12181 ccctgttgtt ggagaagagc tggcaggcag ccgttccagt gtgtaccgct ttcacctgga
    12241 ctcgcccatt ccattccaga agtcgttcaa agctaccatc gagcatggac acgcaaatgc
    12301 tcgctctgac aactattact cagtcgcata ttggtaccag tcagaaccac atgaggcctt
    12361 tccgccgctg cctcctgtag agcagcggat tccggcgctg cagtttgttg gtgggcctgg
    12421 aaacagtaca gctaaaaaat aaatggcctt ctgaattgat tcaggcccca cacgtttaca
    12481 gaagtgtggg gcacttcatc tgagtgtctc tgcaagtttt tccaaaaaaa ccttttgccc
    12541 aggcagaatc cgtttcctcg cctcattcag agaaaaccac tcccagcggt ccacttccgg
    12601 aaattcgacc gttctgccgg agcggggcgg ccactccatc tgaaatgtgt tgctgcgtaa
    12661 gcctgcagga ctgcaatctc cttgcaccgc ccatacactt acaagtttgc ccgccggctg
    12721 cttttcttct cccagcggga gaaagtctcc gctgacttcc agtccggttt cttcctggaa
    12781 ctcgcgtcgc gcgactgtaa gaggatcttc gccgggcaga tactcccctt tgggaacaga
    12841 ccatgcgccg aggtctttct ttgcccagaa cgggcctcca ggatggacga gaaagacctc
    12901 ggttccattt gcctggcggc gatacatcag gattcctgcg ctgcgcttcg gcataatcat
    12961 tattctcaat caacactcaa ccaaagtggc gcgtataaaa tcaaatcctc atgggaagca
    13021 caccggtatt gcaggatgaa ctgcaaggct ctgtcttcgt gctgattcaa tttgatgtgt
    13081 gcgaagagat acgcctggac cgtctgaagc aaatcttcag cgcgcagaca gtgcagcgcc
    13141 ccgcgctgaa acatgcattg cccagctaca tccgctatga gcggccgccc gttgtggaag
    13201 caacgggacc gctggtgctt gagagcggcg aacgccttca cgggcaaatc aaatactacg
    13261 attacggcgt tgtcagcgtg atccttgaac tgcccttcgc tggtaattgg gagaagctgg
    13321 tccagctttc aagccgctgg gtctgggacg tggatttttc cgggcaggta ctgcgcattg
    13381 taaaagagcg gctgcatcag gccgcagatg ctcttgcaaa gccatatgag gaatggctga
    13441 gtgaggacta ctttatcttt catgtggcgg aactggctgg cccactcact gctgcgcagg
    13501 tcatacagct tcgcggaaaa gaaatttcac aaatcgtgcg cggagatgca acggaattat
    13561 cagacagtga acgccaggag gtcctgcaat ccggcatttc ctatggaatc aaagaccttg
    13621 ctgtgattgg ttggaacgcc gcttttctct acgacacgca gtctggcgca gacacggcca
    13681 tccagctatt ggaatatgca aattcgcaat tgctggagtt tcgtcattat gacgagcttc
    13741 tgacaaacga actggagagc gtatacaact cgctggagaa gggaactgga ttctttgcgc
    13801 gctggcggct ggcccgctct gctaccaaac tgaacaccgt gctgcttgaa gtgacggagc
    13861 tgacagagca cgctgacaat gcgatcaagt ttttgagcga catgttttca gcgcgcctct
    13921 ataaggttgc tgcggccaaa gtcggcgttc cggactataa aaatctggtc accaataaag
    13981 tgcagacagc gcgggagctt tatcacttta tggtggacca gttcaatcag gggcgcgcct
    14041 tcttccttga actgacagtc gtcatcattc tgatcattga attgctttat ctgtttcgag
    14101 ggcacccgtt ctaatcgccc caggatgcaa gctgcttcag gcgcggaaaa tagagtgcaa
    14161 agcggaggcg ggtatctttg ccgtggatct tttgcaacgc ctttccatac atcgcaagct
    14221 gagggccgta ttgttcacgt tgtgcagcca aaaagcgtgc gacatctgtg ccagtgtatg
    14281 ggatggtttt gtaatccacc acccagatga agtctctccc ggcggacaaa ggcccgctgc
    14341 ctgcgcgaaa gaccctgtct gcgcggaccg tgcgcagagc gccttctatc catccggtcc
    14401 acgacgcctc agattgtgca tcaggatgtg gggccagcag ccattgtccc tgcggatctt
    14461 cgatcgcggc gcttaccgat gcaacgactt cgttcaatac gtgcgatgcc tcttcagcgg
    14521 gaagggcagc cgcgcgcaac atggccgaag catgacgttc aatccataca gcgatctctg
    14581 ccagagacac tccttgcatt cgtgacaacc gttccagcaa agtatgaatt atgctgcctg
    14641 tcagccgctg catgcgccca ccctccgggc gctcaaaaag ttcgtaatct tctactgaag
    14701 aaacaggccc ggggacttta atatttgcgc cacgttgagg caggactacg tccattggca
    14761 accgatgcag catcaatggt gggtgcgaag gggcggcctc agcggcaata tctaccacgc
    14821 cgggttgtgg agcagggaag gaaataacat tctgtcgttt tttctcttca tatgcagaag
    14881 caaattccgt ttccagggcc ggccatgcag cgcacaacag agtatttgct gcggaagaca
    14941 atttatcatc attcgtaacc gtagcagttc cagataagtg gagctcgctt cttgcgcgcg
    15001 tgcaggcaac ataaaaaact cgtttcagct cttcctcttc ccgcaggtcg cgcagtttgc
    15061 gcacccagcg atacagcgga tgcgtgccgt ttccgcggct gtctatgggt gcaatcagca
    15121 gctcatcttc attgttgttg gccgtattgg cgcgctgaag catgacgatg agtggcttgt
    15181 tatcgctgcg ggtagtgcgt tcgagtccgg gcacgatgac tacatcaaag ccaagtcctt
    15241 ttgctttgtg gatggtcatc aactgcacac ctgcgcggtc actgacgctc ggatcaggct
    15301 gggcaaacag acgctccagt tgctggtcca gctcatcgcc caggcaggca atgccgtcgg
    15361 cggagatgct gtcgagcatg gaaaagaata cccgcgcatt ttcttgttgg gttgcatcca
    15421 gacacagcgg tccacccaat gaatgccatg tgcgctcaat ccatgtggag agagaaagtc
    15481 cctgttgata gcgggtctcg agggcgcgct cgagcactgc aaatgttcgc tcaagccgtt
    15541 gctgtccgtc cttgctcagt agtacgcgcc gttcctcaac caggcgtgat accggaacac
    15601 gcgcaaaacg cctgtcgtct tctccacaaa gagtgtgcag atcttgcaga gtaagcccgc
    15661 accacggagc acgcaaaaca gaaagccacg caatgcggtc catcgggtgc aacagtgcgc
    15721 gggtgagcga tagcagatca cgaatctcct gccgttcgcc cagcaattcg atttcaacag
    15781 cgcgatacgg aatctttttc tctcgcagct cccgtgcaat cagtggcaga tgttggcgtg
    15841 cccgtgccag cactgcgacc gtgaatgtat cctctgggct tgtctcggct ttcctgatgc
    15901 gaggcaggtg ttgctgaatc acctgaatca cctcattggc ttcctgcttc gctgcccgct
    15961 ctgtttcggc tttatctcct ttgggaacaa agtgtggatg caggtgcaca gactgtcccg
    16021 gcaatgctgg aactacagct tcagacctgg aaaaggggac cgcggccgat ccgggcgtgt
    16081 gatggtttgc gaagatattt gcaaaccagg cattcagcgg ctctgtaagt cccgcgtggg
    16141 agcggaaatt cgccgacaag gaaatgggcc tgaacgaatg ttcttcaatg ccatgtcgct
    16201 gtacctgttc aaacagctct acctcggcct gacgaaacat atagatggac tgcatcgggt
    16261 cgcccaccag aaagcaagtc cgccggccat tgtcccccca tgcgcgcagc agcatctcaa
    16321 gaagacgtat ctgacggcgc gatgtgtcct gaaattcatc cactaacaag tggcgaatct
    16381 gctcgccaaa acgaagaacg ttttcagttg gattttcaag gacctgcaat gccgcaatgc
    16441 caagctcaac aaagtcaatt atgtttcgtt ccgcaaagag cacgcgcaat tcggcgactg
    16501 cgtagcgcag aacaatgaag agctggcgca gggtgtgcca ctggccttcg tcgtagtgga
    16561 gtggtggaag tttccgaatc gcacagagcg cttcccgcag accagtgatt ccagaaattt
    16621 tcttgagcag tccgatcatt tgttctttgt attgctgctc agttgaattt ttatgatttg
    16681 gggtggaagg gaagccttct ctgaccgtca cggttttgcg ccattcgtct tcattgttca
    16741 gcagaaggcg gcagatgcag ttccattggt ccagaaattg ctcggaagtt cccggcagag
    16801 cacgaatgtg cgcaattgct gccagatcaa gattattgac atttgcactt gcgtaatttg
    16861 agagtcgaag cagatgatca gctaagagtg gttctgccgc aatatgctgg tgggcccgct
    16921 tcaatgcctg gcgcacggcg ctcctgaatg gcgcttccag cttgcgcctg acttcggccc
    16981 attcctcctc gcttatctcg ttgctcagcg gaaaaacatg cagccactgg tcgcgtttcg
    17041 caagcatttc agcaatgagc cgttgcgcat cgggcagatt gttgtcgcgc aacgccagca
    17101 ggtgcatgag cgcggaatga atttcatcct tggtgctgcc cagattggcc agtgtgcgcc
    17161 gtgctgcttc atcgtaaagc gcgcgtgcgt cttcctgcgg ttgcagatgt ccgcccatgc
    17221 gcgcgagcaa cggctgttcg tgcgcaatgc gcatgcagag agaatcaatg gtctggatat
    17281 tgagtctttg cggttgttcg agcagctgcc atcctcgcgc ttcatcctgg gccagcgctt
    17341 ttcttgctgt ttccagagca gggtcatatt tgcctctgcg tgctttctca agttgctgca
    17401 aaatacgcga gcgcatttcg gcagtagctg cgcgggtaaa ggtgatggca aggacctgct
    17461 ccggctcatc cacctcggcc agcagcttca aaaagcgctg cgtcagaagc tcggtctttc
    17521 ctgatccggc aggcgcctgc acaatgcagg aaatagcagt gttcagtgct tcgtcgcgct
    17581 gggcctggtc tggagggacc aattgcagct tactcatcgt ccggctcctc tccatccgtg
    17641 gccaggtcaa gctcggcgat tctgcagagt ccaggcagtg ggcaatactt gcaggtcttt
    17701 ggcccttctt tcgggtctac ggatgcttcg ccgcgcagaa actcttcggc cagaccaagc
    17761 aacgcgcgct gccagccctc tctcattttg atgttgtacg gcttgttcac caatgggctc
    17821 ttggccgtaa gaccggcttc aaactgcgcc tgcgcgttcg ccacgtgacc cgcaaattct
    17881 gtcttccctg cgcgaattcg cgcgaagagc agtccgctca cgttctcaac attgccatag
    17941 atcgcgtata gcggaagctg cggttccttc atccgttctc cctgccaatc tgccgcagaa
    18001 acatctcccg tcttgtagtc aatcagcaag cggcttccgt ccggcaatag atcaatgcgg
    18061 tcagcgcgaa gattgagctt cagcccgcca acatgcacgt ccttcaattc ctcttcacgt
    18121 ctttcgacct cgaatggcag ccgttctgcc tcgcactgca tccactcacg caaacgcacg
    18181 agcaaccgct gttgttctga ttccagataa gaggtcatcc acgcatcgcc tgcattttct
    18241 ttcataagcc cggcaaaagc atgactgatg tggtaacgaa gcacctcatc cagcctttgc
    18301 gcagccatca cctgcttcag gtcttcaagg ccggtcatgc ggaaagtctc tggtgtcttc
    18361 ggcgaccaga tattctccag aattctgtgg atcagattgc cgcgatccat cgcgctcagg
    18421 ccccattctg cacggttcag cggctttgct tgcagacgcc gtgttgcaaa ggcctggaaa
    18481 gggcatgcgg cctgttcctt cagaatgctg gccccgcccg cggcccggtc ctggggccag
    18541 tgtgcgattt cagaggactg gtagagcctt tccaactcct gcgcgagcag tgccggctcc
    18601 tcaccatgca attgcgcgcg cagatcatgc gtggagagcg tcttgtagtt tgttccaaaa
    18661 atccgggcca gcaacggaga aggccgctgc tcgccttcct tgttctggcg cgcgtagctg
    18721 aagacgaatt ccggagcgct gtttgcgatg cgttgtgtaa ctgtgtgtgc cagcccg
//
LOCUS       DASRBV010000235         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_524634, whole genome shotgun sequence.
ACCESSION   DASRBV010000235 DASRBV010000000
VERSION     DASRBV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524634"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="dgt"
                     /locus_tag="VFA02_08830"
     CDS             complement(<1..663)
                     /gene="dgt"
                     /locus_tag="VFA02_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HZU09998.1"
                     /translation="MHLPPGCAVSDSDALLTARVFPEDPHPYRSAFQRDRGRIIHARA
                     FRRLAGKTQVFTHRYSDHFRSRLTHTMEVAQIARTVAAALGLNEDLTESLALVHDIGH
                     PPFGHAGERALDEALKKFDLGFDHNLHALRMVESFESRYAAFRGLNLTLAVREGIIKH
                     SRDYSANEHPELTQYYLDKRPPLEAQLIDLVDEIAYLTADLDDGMEAEILCLEDIRQH
                     ERL"
     gene            complement(667..2802)
                     /locus_tag="VFA02_08835"
     CDS             complement(667..2802)
                     /locus_tag="VFA02_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU09999.1"
                     /translation="MKIHGKARLASHHRLLSVSTLALAGFYSPQIFAQSAPASPQSKP
                     QATTATQPQQSDRASAYYHYTLAHMYEEMATTYGRPEYANRAIEEYKLALNADPDSPY
                     LNAHLAELYFDTGRVRDAIAAAQSEIQKDPDNLEAHKLLGRIYLRSLGNMQNGPSEQM
                     LKKAIDEYKEIVRLEPGSTEDHLFLGRLYMLDHDSVHAEEQFKAAQKIDPDSEDVVLN
                     IAGLYSEQGDFQRAAQILQALPEDDQTPKTLYALGQIYDQLKDNKKALDAYQKAYDLE
                     PDNLDVERALAQSQMNNGQKDAALKSFKDISSGDPSDAQSLLSIAKIERQQGKYEDAL
                     ASLKKAKDLVSDSLEINFEEALDYDALGQFDQAAQVLSKLVADSEHPSGAYSQGEKNN
                     RAVFLDRLANVYREQGKTDQAIATYQKMADLGGDYTSRAYQNMVNVYRDAHQYDKATA
                     IAREAVTRLANDKDNLRDAKLTLASQLCDTGKADEGISLARSLLNGNTDNDRMVYFQM
                     AEMYIRMKRWKDAEDIINQADQISTKPDDKANVYFERGELEERQKHYDQAESWFRKVL
                     DLQPNSAVTLNYLGYMLADRGVKLNEALAMIKKAVQLDPQQYAYLDSLGWVYFKLGDY
                     TLAEENLTKALARNSGDPTVHDHLGEVYEKTNRLKLAAAQWEQSLNYYAHSLSADADP
                     SDVSKVQKKLDSARVRLAKESSNTLASPK"
     gene            complement(2951..>4117)
                     /gene="purH"
                     /locus_tag="VFA02_08840"
     CDS             complement(2951..>4117)
                     /gene="purH"
                     /locus_tag="VFA02_08840"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZU10000.1"
                     /translation="AKNFEDVAIVTSPADYAALAEELDANQGALGRETRWRLAKKAFA
                     TTAAYDTAIANTLEQIASPEPAEAPAQFVDPATAAFPDALRINYPLAMPLRYGENPHQ
                     KAALYSDGSETGVAGGKQLQGKELSYNNLVDLDACWELVQEFDETAVAIIKHTNPCGA
                     ATGHTVLDAYKRALEADPVSAFGGVIGINREVDEAAAEEIAKLFVEAIAAPGFSEGAR
                     ARFATKKNLRLVEIQPAQTLRVIKQVSGGLLLQDADRGHVTAAGLKTVTERKPTDDEL
                     AALLFAWRVCKHVKSNAIVYARNGQTISVGAGQMSRVDAAKFGAMKAVLPLKGCVAAS
                     DAFFPFPDGLETVAAAGATAVIQPGGSVRDEEVIAAANKLGVAMVFTGMRHFRH"
BASE COUNT          789 a         1081 c         1270 g          977 t
ORIGIN      
        1 gagacgctca tgctggcgaa tgtcttcaag gcagagaatt tcggcttcca tgccatcgtc
       61 aaggtcggcg gtgagatagg cgatttcgtc taccaggtca atgagctgcg cttccagagg
      121 aggccgcttg tccagatagt actgcgtcag ctccggatgc tcatttgcgc tgtaatcacg
      181 tgaatgcttg atgatgcctt cgcgaacggc aagggtaaga ttcaatccgc gaaaggcggc
      241 atagcgcgat tcaaaggatt caaccatgcg cagggcatga aggttatggt caaacccaag
      301 gtcaaatttt ttaagcgcct cgtccagagc gcgctcacct gcatgtccga atggcggatg
      361 gccgatgtca tgcaccagcg ccagggattc ggtcaggtct tcattcaaac cgagcgcggc
      421 agcaacggta cgggcgatct gcgctacttc catagtgtgg gtaaggcggc tacggaagtg
      481 gtcagagtag cgatgggtga aaacctgcgt tttgccggcc agacggcgaa aggcgcgggc
      541 atgaatgatg cgtccccggt cgcgctggaa ggcagagcga tagggatgcg ggtcttcagg
      601 aaagacacgc gctgtcagca gcgcatccga atcagaaact gcgcagcccg gaggaagatg
      661 catggattat ttcggagatg cgagggtgtt ggaagattcc ttcgccaggc gcacacgcgc
      721 gctgtcgagc ttcttctgca ccttgcttac gtcgctgggg tcggcatcgg ctgagagcga
      781 atgcgcatag tagttgagcg actgctccca ttgcgcggcc gccaacttca ggcggttggt
      841 cttctcatag acttcgccca gatggtcatg tacggttgga tcgccactgt tacgggcgag
      901 cgctttggtg aggttttctt ctgcgagtgt atagtcgcca agcttaaagt agacccagcc
      961 gagagaatcg agataagcgt actgttgcgg gtcgagctgc acggcctttt tgatcatggc
     1021 cagcgcttcg ttcagcttta caccgcggtc ggccagcata taacccagat agttgagggt
     1081 gacggcagag tttggctgaa gatcgagcac cttgcgaaac cagctttcag cctggtcgta
     1141 gtgtttctgg cgctcttcca gctcgccacg ctcgaagtaa acattggctt tgtcgtccgg
     1201 cttcgtggat atctggtcgg cctgattgat gatgtcttcg gcgtctttcc agcgtttcat
     1261 ccggatgtac atctcggcca tttggaagta gaccatccgg tcattatctg tattgccgtt
     1321 cagcagggac ctggccagcg aaatgccttc atcggctttt ccggtatcgc agagctggct
     1381 tgccagagtc aacttcgcat cgcggagatt gtccttgtca ttcgcaagtc tggtaacagc
     1441 ttcgcgcgcg atggcggtgg ctttgtcgta ctggtgcgcg tcacggtaga cattgaccat
     1501 gttctggtat gcgcgcgagg tgtaatcacc accgaggtcg gccatcttct gataggtggc
     1561 gatggcctgg tcggtcttgc cttgttcacg atagacattg gcgagccggt caaggaagac
     1621 ggcgcgattg tttttttcgc cctgagagta tgcgccgctg ggatgttcgg agtcggccac
     1681 cagtttggag aggacctgag cggcctgatc gaattgtccg agggcatcat agtccagtgc
     1741 ttcctcaaag ttgatttcga gggagtcgga aaccaggtct ttggccttct tcaaggaggc
     1801 aagagcatcc tcatatttgc cctgctggcg ctcgattttg gcaatgctca gcagagattg
     1861 cgcgtcgctg ggatcgcccg aggagatgtc cttaaatgac ttcagcgcgg catctttctg
     1921 gccattgttc atttgtgatt gcgcaagggc gcgctccaca tcgagattgt caggctcgag
     1981 atcgtaggcc ttttggtagg catcgagcgc tttcttattg tccttgagct ggtcgtaaat
     2041 ctggccgagc gcgtagaggg tcttgggggt ctggtcgtcc tccggaaggg cctgcagaat
     2101 ctgtgctgct cgctggaagt cgccctgttc gctgtagagt ccggcaatgt tgaggacgac
     2161 atcttcggaa tcgggatcaa ttttttgcgc ggccttgaat tgttcctcgg catggacgga
     2221 gtcatggtcg agcatgtaca ggcggcccaa aaacagatgg tcttccgtgc tgcccggttc
     2281 cagccggaca atctccttgt attcgtcaat cgcctttttc agcatttgct cggaagggcc
     2341 attctgcata ttgcccaggg agcgcaggta gattcgaccc agcagcttgt gtgcttccag
     2401 gttgtcaggg tccttctgga tttcagactg agcagcagca atggcgtcgc ggacgcgtcc
     2461 tgtatcaaag tagagttcgg ccaggtgcgc gtttagatag ggtgagtcag ggtcggcatt
     2521 cagggccagt ttgtattctt caatggcgcg gttggcatat tccgggcggc cataggttgt
     2581 ggccatctcc tcatacatat gagcgagtgt gtagtgataa taggccgagg cgcggtccga
     2641 ctgctgcggt tgcgtggccg tcgtagcctg cggcttgctt tgtggggaag ctggcgcgct
     2701 ctgcgcaaaa atctgaggac tgtagaagcc tgccagagcc agcgtagaaa cagaaagaag
     2761 gcgatggtgc gatgccaaac gggccttgcc gtgtattttc aagaagaacc cctgccagat
     2821 gtcccggcct gaaccagttt catgcttaaa gacgattgta aaccggattg ggcttcatgg
     2881 gacagggaca ggtttcaggg cacaagtctc agaaatcctg ctcctgcgaa ctatgccctg
     2941 aggctgtaaa tcagtggcgg aagtgccgca ttcctgtaaa gaccatggcc acgcccagtt
     3001 tgttggccgc agcgatgacc tcctcgtcgc ggacggagcc tccaggctga atgacggccg
     3061 ttgcgccagc agcagctacg gtttccaggc cgtcaggaaa gggaaagaat gcgtctgagg
     3121 cggcaacaca gcctttgagc ggcaggactg ccttcatggc gccaaacttt gcggcatcca
     3181 cgcggctcat ctgtcccgcg cccacgctga tagtctgccc attacgcgca taaacgatgg
     3241 cgtttgactt tacgtgcttg cagacacgcc aggcaaagag cagcgccgcc agctcgtcat
     3301 cggtgggctt gcgttcggtc acggtcttta ggccagcagc agtaacatgg ccgcggtctg
     3361 catcctgtaa aagcagtccg ccggagacct gcttgatgac gcgcagggtc tgcgcgggct
     3421 ggatttccac cagacgcagg ttctttttcg tggcaaaccg ggctcgcgcg ccttccgaaa
     3481 agccgggagc ggctatggct tccacaaaga gttttgcgat ttcctcggct gcggcttcgt
     3541 ctacttcgcg gttgattccg atgactccgc caaaggccga aaccgggtcg gcctcaagcg
     3601 ctcttttgta tgcgtccagc acggtatggc ccgttgccgc gccgcacgga ttggtgtgct
     3661 tgatgatggc gactgcggtc tcgtcgaact cctgtaccag ctcccagcag gcgtccaggt
     3721 ctaccaggtt gttgtagctc aactccttgc cctggagctg ttttccgcct gcgacgcctg
     3781 tctccgagcc atcggaataa agcgctgctt tctggtgcgg gttctcgcca tagcgcagcg
     3841 gcatggccag tggatagttg atgcgcaggg catcaggaaa cgccgctgtg gccgggtcaa
     3901 cgaattgagc aggggcctcg gccggctctg gtgaggcaat ctgttcgaga gtgttggcga
     3961 tggccgtgtc gtaggcggct gttgtcgcga aggctttctt tgccagacgc cagcgggttt
     4021 ctcgtcccaa agctccctga ttggcgtcga gttcttcagc gagcgcagcg taatcggcag
     4081 gggatgttac gatggccacg tcctcaaagt tcttggc
//
LOCUS       DASRBV010000236         8567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_69021,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000236 DASRBV010000000
VERSION     DASRBV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69021"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="ahcY"
                     /locus_tag="VFA02_08845"
     CDS             complement(<1..1274)
                     /gene="ahcY"
                     /locus_tag="VFA02_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZU10001.1"
                     /translation="MATTIATQHHVKNLDLADQGRKRIEWANQSMPVLQTIRKEFIKN
                     QPLKGIRIAACLHVTTETANLMITLRDGGADVALCASNPLSTQDDVAASLARDFNIQT
                     YAIKGEDNDSYYSHILSAIDHKPHITMDDGCDLVHLLHTKRQDALEGVIAGTEETTTG
                     VIRLRAMSKDGVLRYPVIAVNDALTKHMFDNRYGTGQSTIDGVIRCTNVLLAGAKFVV
                     AGYGWCGRGLAMRARGMGAEVIVTEVDPTKALEAVMDGFRVMTMMEAAKQGDVFVTVT
                     GNKSVIRQEHFEVMKNGAIVANSGHFNVEIDIPALERIASSKRATRDFVDEYALRDGR
                     KIYLLGEGRLINLASAEGHPASVMDMSFANQALSAEYLVKNHASLEKKVYAVPQELDK
                     RVARLKLDAMGIGIDRLTPEQEEYLASWSEGT"
     gene            1364..1651
                     /locus_tag="VFA02_08850"
     CDS             1364..1651
                     /locus_tag="VFA02_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU10002.1"
                     /translation="MAGKGAQLTARQVTRISRALADPHRYEILKKIAAKGDCPCQDLR
                     EILPISPATLSHHIRELETAGLIEIEREGKFAHLSLRRDVWQGYLNQLTKI"
     gene            1731..2480
                     /locus_tag="VFA02_08855"
     CDS             1731..2480
                     /locus_tag="VFA02_08855"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU10003.1"
                     /translation="MSKLQGKVAVVTGASKGIGVAIARQFGAEGASVVVNYASSKAGA
                     DKVVADITAKGGKAIAVQADLSKPSEIGKLFEETKKAFGKLDILVNNAGVYEVAPLEG
                     ITEEHYKKHFDLNVLGLILATQEAAKLIGPEGGSIINISSTITSITPPNFAVYAATKG
                     AVDTITRTLAKELGPRKIRVNAINPGMVVTEGVQAAGYDKGDMRTYLESITPLGRIGQ
                     PEDIAPAAVFFASQDSSWITGEVLRVAGGVR"
     gene            complement(2517..3773)
                     /locus_tag="VFA02_08860"
     CDS             complement(2517..3773)
                     /locus_tag="VFA02_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10004.1"
                     /translation="MWSEFESEGVAGRFQLGKLVRSEGRYGWFETVVDGKPAIIGVIE
                     ALNDEDALLDRLKAAEKVRHANVVAILETGTATVKGTPLVYAVMESTEENLEDVLRVR
                     ALSTEETRQIAEDLVKALKAIHKEKLVCGEIEASGVLAMGDVIKLRSDKLQNAAGPTD
                     IAFAALVSKDVQALGMLLYRCLTLKRPQTEGDDPSVELLPQPFSQMVRRALRGQATVE
                     EMEALLRQPVSALKAAESTVVEVPAALAHESTGSSGRAGSMAWMVAGVVLLALVLVFA
                     FRALLHSSKDVSAQPAPPSVSQAKVQTPVAGPVHVQKPVATTPVRPLSAPVPTMATQT
                     TRKNTIWRVVAYTYNHQDQAEHKAQTINSKYPDLKAEVFSPKGNSAPYLVTLGGPMER
                     DAAFQLRNKAVSEGLPRDMYAQNYSH"
     gene            complement(4109..4423)
                     /locus_tag="VFA02_08865"
     CDS             complement(4109..4423)
                     /locus_tag="VFA02_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10005.1"
                     /translation="MATLPVSSPAEKYLVAFVIAGDDVFKVTADNVIQAGAGAGTPAF
                     EGVAKLKAAAALFQLTDIHNQRPNELRKMAREMAIEGMTLLARASNVPRLTAAAPESS
                     VA"
     gene            complement(4713..5552)
                     /gene="accD"
                     /locus_tag="VFA02_08870"
     CDS             complement(4713..5552)
                     /gene="accD"
                     /locus_tag="VFA02_08870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZU10006.1"
                     /translation="MSWFKRQENKIESAGGKTVRTEGLWLKCEECSQIIFKADVEANL
                     QICPKCGHHFRMGARQRIESLLEPGYEIVDTELKSTDPLNFTDLKPYKSRLKKAQQET
                     GLNDAILNAVGKLGPHDVVLSVIEYSFIGGSMGAVMGETIARAADRAYAQRWPLIVIS
                     ASGGARMMEGIVSLMQMAKISATLSRLDDAGVPYISVLTDPTTGGVTASFAMLGDLNI
                     AEPGALIGFAGPRVIEQTIRQKLPEGFQRSEFLLEHGFLDAVVSRKELKQYLVRALDF
                     MKS"
     gene            complement(5673..6221)
                     /gene="cobO"
                     /locus_tag="VFA02_08875"
     CDS             complement(5673..6221)
                     /gene="cobO"
                     /locus_tag="VFA02_08875"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZU10007.1"
                     /translation="MTESNEQSRRGLVLINTGPGKGKTTAAMGTALRAVGNGMRVLML
                     QFLKGSWHYGELDAVEAFDGNFVMRQMGRGFVKIGGAETDPEDVRLVEEAWEEARKAI
                     LSGEWDMVVLDEINYAISYGMLDPAKVAETLKERPEMVHVILTGRNAHPSLVEIADTV
                     TEMREVKHAYQKGILAQRGIEY"
     gene            complement(6229..6669)
                     /locus_tag="VFA02_08880"
     CDS             complement(6229..6669)
                     /locus_tag="VFA02_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZU10008.1"
                     /translation="MKLLLAWIGTGRPSRNEWEVEYLRRIQPYASAEMAVFRSEDEFF
                     AKAVAQQARTAPVLVLLDSRGRQFTSEQFAEWLGRRRDEGRQRIIFAIGPADGWSEAA
                     RKRASLLLSLSAMTLPHELARVLLCEQVYRAFTILSGHPYHTGH"
     gene            7083..>8567
                     /locus_tag="VFA02_08885"
     CDS             7083..>8567
                     /locus_tag="VFA02_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU10009.1"
                     /translation="MNSRYLKAEVRLFAGLLVLLLAWSVFPEKVFAQNVYAAIHGTVT
                     DPSGAVVSNASVMVTNTSTGISSQQTTDNKGYYIFPQLAIGGPYTVSITATGFQSFKT
                     VGLVLQLNENRQVDAVLQPGAVAQTIEVTANAIQVETSDTQLKTTVTSNQIEQLPLLG
                     RDPTALQKLTPGTVESSDRFGNFSANGSQTQENAYLLDGADINDTPLQTNSVPINPDA
                     IGETTFVTGTQNPEYSRNSGAIINQTLKSGTNSFHGDAFEFYRDTFLNNGNYFSTTRP
                     VFHQNIFGGTLGGPVIKNRLFFFLAYQGLRNSTATTTVTPVPTDAQLGRNSTGFADLT
                     NDNNVANSGPNTSVGLTSNPLPFTIQGPGGTPCPAGTPWNQCFPGPTYQLPVSDFNSI
                     SSNLLAKYVPSPNFNGKFYNFNAANTAGADQGIIRIDTKVTANDQLWASSIFQSNPSL
                     NALPFTGSTLPGFAQDASRHFKIFSASWTHSFNPTTLNELRAGYY"
BASE COUNT         1958 a         2526 c         2151 g         1932 t
ORIGIN      
        1 tatgtaccct ctgaccagct cgccagatac tcttcctgtt ccggagtaag ccggtcaatg
       61 ccaattccca ttgcatcgag cttcagccgg gctacgcgtt tatcgagttc ctgcgggacc
      121 gcgtagacct tcttctccag gctcgcgtgg ttctttacca gatactcagc cgaaagtgcc
      181 tggttggcaa agctcatgtc catcactgaa gccggatggc cttctgccga agccaggttg
      241 atcaggcggc cttcgcccag cagataaatc ttacggccat cacgcagcgc atattcatcc
      301 acaaagtcgc gggtagcgcg tttggaagaa gcaatacgct ccagtgcagg aatatcaatc
      361 tctacattga agtggccgga attggcgaca attgcgccat tcttcatcac ctcaaagtgt
      421 tcctggcgga tgacgctctt attgcctgtc acggtgacga agacatcgcc ctgcttcgcc
      481 gcttccatca tcgtcatcac gcggaagcca tccatcacgg cctcaagtgc cttggtcggg
      541 tccacttccg tcacgatgac ctctgcgccc atgccgcgcg cgcgcatggc caggccgcgt
      601 ccgcaccagc cgtatccggc gacaacgaac tttgcgccgg ccagcagcac attcgtgcag
      661 cggatgacgc cgtcaatcgt ggactggccg gtgccgtagc gattgtcaaa catatgtttg
      721 gtcagcgcat cgttcactgc aatcacggga tagcgcaaaa caccatcttt tgacatcgcg
      781 cgcagacgaa tcacacccgt cgtcgtctct tctgttccgg caatcacgcc ttcaagcgca
      841 tcctgacgct ttgtatgcag caaatggacg aggtcgcagc catcatccat cgtgatgtgc
      901 ggtttgtggt caatggcgga aagaatatgc gaataatagc tgtcgttgtc ctcgccctta
      961 atggcatagg tctggatatt gaaatcgcgc gccagcgatg ccgccacatc gtcctgcgtt
     1021 gaaagtggat tcgatgcgca cagcgcaaca tccgcgccgc catcgcgcag cgtgatcatc
     1081 aggtttgcgg tctctgtggt tacgtgcaga caggccgcaa tgcgaatgcc cttgaggggc
     1141 tggttcttga taaattcctt gcgaatcgtt tgcagaacag gcatggactg gtttgcccat
     1201 tcaatgcgct tgcgtccctg atcagccagg tcgaggtttt ttacatggtg ctgggtagcg
     1261 atagtggttg ccataaattc tcccgggccc gtcggggctg tgggccgcat aaatacacat
     1321 aagagaatat catccatgtt tgcgtcaaga taggataaga gatgtggcag gaaaaggcgc
     1381 ccagcttacc gcacgccaag tgacgcgcat ctcgcgcgcg ctggccgacc cgcaccggta
     1441 tgaaattctg aagaaaatcg cagccaaagg ggactgccca tgccaggacc tgcgcgaaat
     1501 actgcccatc agccctgcca cgctctccca tcacatcagg gaactcgaaa ccgctgggtt
     1561 gattgaaatc gagcgcgaag gcaaattcgc ccatctttcg ctgcgacgcg acgtctggca
     1621 gggctatctc aaccagttaa caaaaattta actccacaat ctgcgaccaa atttctttag
     1681 cctcatcaat tcgatagtta tcgaattatc aaagcatgga gataagtctc atgagcaagc
     1741 ttcagggaaa agttgctgtc gttaccgggg catccaaagg catcggcgtt gccattgcaa
     1801 ggcagttcgg ggccgaagga gcgtctgtcg tcgtcaatta cgcttcgagc aaggccggag
     1861 cagacaaggt cgtggctgac atcaccgcca aaggcggaaa ggccatcgca gtgcaggccg
     1921 atctgtcgaa gccgtcggaa atcggcaaac tgtttgagga gacaaagaag gcattcggca
     1981 aactcgacat tctggtgaac aacgccggag tctatgaagt tgcgccgctc gaaggcatca
     2041 ccgaagagca ctacaagaag cactttgatc tgaacgtgct tggtctcatc ctggccacac
     2101 aggaagccgc aaagctcatc gggcctgaag gcggaagcat catcaatatc agctcaacga
     2161 tcaccagcat cactccgccg aacttcgcgg tttacgccgc aaccaaaggc gcagtagaca
     2221 ccattacccg cacgctggcc aaagagctgg gcccgcgcaa aatccgcgtg aatgccatca
     2281 atccaggcat ggttgtgacg gagggcgttc aggccgctgg atatgacaaa ggcgacatgc
     2341 gtacctatct tgaatccatc accccgctcg gacgtatcgg gcagcccgag gacatcgctc
     2401 cagccgcagt ctttttcgct tcacaggatt catcctggat cactggcgaa gtcctgcgcg
     2461 tcgctggcgg cgtacgctaa ggcattttta caggcactga agccttccta ttcaggtcag
     2521 tgcgaataat tctgcgcata catatcgcgc ggaagccctt cgctcacggc cttgttcctg
     2581 agctggaacg ccgcatcgcg ctccataggc ccgcccagcg tcacaagata tggagcgcta
     2641 ttgcccttcg gtgaaaacac ctctgccttc aggtctggat atttagagtt gatcgtctgc
     2701 gccttgtgct ctgcctggtc ctgatggtta taggtgtatg ccacgacgcg ccagatggtg
     2761 ttttttcgtg tggtttgcgt tgccatcgtc ggcactggcg cagacagtgg gcgtaccgga
     2821 gtcgtggcca ctggtttctg tacatggacc ggcccagcaa cgggcgtctg cactttggcc
     2881 tgagaaacag aagggggcgc cggttgtgcg cttacatctt ttgaagaatg aagcaacgcc
     2941 cggaaggcga aaaccagcac caacgccagc agcacgaccc cggccaccat ccatgccata
     3001 ctgcctgcac gtccgctgct tcctgtactc tcgtgcgcaa gcgccgcagg cacttccaca
     3061 actgtgctct cggcagcctt caacgccgac acaggctgac gcagcagggc ctccatttct
     3121 tcgacggttg cctgtcctct caatgccctg cgcaccatct gagaaaaagg ctgcggtaaa
     3181 agctcaaccg acggatcgtc accctccgtc tgcggccgct tcagcgtcag acagcgatac
     3241 aacagcattc ccagtgcctg cacatctttt gagacaagcg ccgcgaatgc aatatccgtg
     3301 ggacctgcgg cgttctgcaa tttatcgctc cgcagcttaa tcacatcgcc catagccagt
     3361 acgcctgaag cctctatctc tccgcaaacc agcttctctt tatggatggc cttcagcgcc
     3421 ttcaccaggt cctcagcgat ttgtcgcgtc tcttcggtgg aaagcgcgcg cacacgcaga
     3481 acatcttcca gattttcttc cgtggactcc attaccgcat agaccagcgg cgtgcccttt
     3541 actgtcgccg ttcctgtttc aagaatggcc accacattcg catgccggac cttctccgct
     3601 gccttcagcc ggtcaagcaa ggcatcctca tcattcagcg cctcaatcac gcctatgatt
     3661 gctggtttcc cgtccacgac tgtctcaaac cagccatatc gcccttccga acgcaccagc
     3721 tttccaagct gaaaacgacc agccacgcct tccgattcaa actcgctcca cattcaacta
     3781 ctcccatcca ttcgccgatt gaatatttcg gccccacaca caacagaaga acgaatgtat
     3841 tgtcgttctg agcgaagcgc agtacagggc cccaaaacgc gtacagtacg cattttgggg
     3901 cggtgaagcg cgaagtcgaa ggagctgcat ttttcaatct ttcacgcgcc ggcatctctg
     3961 cgcttccgga aaaccctgca gcacctgtgg atcttcattc aactgttctg tttatctggc
     4021 ctgcgctttt gcaacaggcc cttctcaggc aggaaaaagc aaaaggaaag aaaaaatccg
     4081 tatgaagata ctatagcctg cttcaaccct atgctactga gctttccgga gcggccgcag
     4141 tcagtcgtgg aacattgctt gcgcgcgcaa gcagagtcat accctcaatg gccatttcgc
     4201 gcgccatttt gcgcagttca ttggggcgct gattgtgaat atcggtaagt tgaaacagcg
     4261 ccgccgcggc cttcagcttg gccacgcctt caaatgccgg agtgcccgcg cccgctccag
     4321 cctggatcac gttgtctgcg gtgactttaa agacgtcatc gcctgcaatc acaaaagcca
     4381 ccagatattt ctctgcaggt gaggaaacag gaagagtcgc catagataca tccttcttga
     4441 aatagttgtc ttacatcgct agtaaagaac agatgaagcg cagaattcca tcgtagtcag
     4501 cgcatttccc gtacctgttt tggttccccc tccggttgaa caatctatcg ggcgcaacga
     4561 gtccttgctg ctcctacata tcgctacaca aaacgacacc caatggatga aaagagcgcg
     4621 tagcaaaaag gctggccgac gcaaaccaat ccgccgtgat gctatggacc gtgtccggca
     4681 ggccctggaa acgctgtgga gtctcgcgaa tatcagctct tcataaaatc cagcgcgcgg
     4741 accagatact gcttcaattc tttgcgtgaa accacagcgt caagaaaacc atgttccagc
     4801 aagaactctg agcgctgaaa gccttcgggc agcttctgcc ggatggtctg ctcgatgacg
     4861 cgggggccgg caaagccaat cagagcgccc ggttcggcga tgttcagatc acccagcatg
     4921 gcaaagctgg cggtcactcc tcccgtcgtc gggtccgtca ggacagagat ataaggtacc
     4981 cctgcatcgt ccaggcgaga gagtgtggct gagatcttcg ccatctgcat caggctcaca
     5041 atgccttcca tcatgcgcgc accacctgag gccgaaatca caatgagcgg ccagcgctgt
     5101 gcatacgctc ggtccgcggc gcgggcaatc gtctcgccca tcaccgcgcc catgctgccg
     5161 cctatgaacg aatattcgat gacggaaagc accacatcat gcggccccag ctttcccacg
     5221 gcattgagga tggcgtcatt cagccccgtt tcctgctggg ccttcttcag gcgcgacttg
     5281 tacggcttga ggtcagtaaa gttcagcggg tccgtggact tcagctctgt gtccacaatc
     5341 tcgtatcccg gctccagaag gctttcaatg cgctgccgcg cgcccatccg gaagtgatgg
     5401 ccgcacttgg ggcagatctg cagattggcc tctacgtcgg ccttgaagat gatctggctg
     5461 cactcctcgc acttaagcca aagcccttcc gtgcggaccg tcttcccgcc agcgctctca
     5521 atcttgtttt cctgccgttt gaaccaggac atgccacctc actcaaaaac tcttgtcatc
     5581 ccgggcagct atcacttctg cccctggaaa tccctgtgtt attacgaccg gagcaccgcg
     5641 aggtgctggg cggagggacc tgtatttcct acctaatact ctatcccccg ctgcgccaga
     5701 atgcctttct gataagcgtg cttcacttcg cgcatctcgg tcaccgtatc tgcaatctcc
     5761 accagagaag gatgcgcatt tctacccgtc agaatgacat gcaccatctc cggacgctcc
     5821 ttcagcgtct ctgccacctt cgccggatcg agcatcccat agctgatggc gtagttgatt
     5881 tcatccagta cgaccatgtc ccattcgccg gaaagaatgg ctttccgtgc ttcctcccac
     5941 gcttcttcca ccagccggac atcctccgga tcggtctccg ccccgccgat tttgacaaac
     6001 ccacgtccca tctgccgcat gacgaagttg ccgtcaaagg cctcgaccgc gtccagctcg
     6061 ccgtaatgcc atgagccttt caggaactga agcatcagca ccctcatgcc gttgcccacg
     6121 gcgcgcaggg cagtacccat ggcagcggtg gtcttgccct tgcccggccc ggtgttgatc
     6181 agcaccagtc cccggcggga ttgttcattg gattcagtca tggcgaaatc aatggcccgt
     6241 gtgatacgga tgcccagaaa ggattgtaaa tgcccggtag acctgctcgc agagcagcac
     6301 ccgcgccagc tcatgtggca gggtcatggc cgaaagcgaa agcagcaggc tggcgcgctt
     6361 gcgggccgcc tctgaccagc catccgccgg accaatggca aagatgatac gctgccgccc
     6421 ttcatcgcgc cgtcgcccca gccactccgc aaactgctct gaggtgaact gcctgccgcg
     6481 gctatccaaa aggacgagca ctggcgccgt ccgcgcctgc tgcgcgaccg cttttgcaaa
     6541 gaactcatcc tctgaccgga agacggccat ttcagcagaa gcatagggct ggatgcggcg
     6601 cagatactcc acctcccact cattgcgaga gggccgtccg gtgccaatcc atgccagaag
     6661 cagcttcata gggtgggtca ttcatagagt acgcttcgca ctgatttgac cattcgcctg
     6721 cgttctctac caaatatgtg aggtgggtag ccgggaatct ttaagagcaa gtcgaattct
     6781 gtttttcata gtgaaaagca gctaggttct gagatttcga acaccgctat aaattactta
     6841 ggcaatgttt atttactaaa cagttagtag ttaaacacag ttatgtgttt aactttgata
     6901 aacttatcat ctcatcataa aagataggct ataaggtatt gaaattgcta cccctggtac
     6961 acagagaaag ggggagtcta cggtaaatag cctggtggca tccgggcaga ctacatttct
     7021 ggttcttctg tcccgaatgc tcaaggttag ctttccgtag tctcctgagg gagaggggtg
     7081 gaatgaatag ccgatactta aaagctgagg ttcgtctttt tgcaggccta ctggttcttc
     7141 ttcttgcttg gtctgtattt cctgaaaaag tctttgcaca aaatgtctat gctgcgatcc
     7201 atggcacggt aaccgaccct tcaggggctg ttgtgtccaa tgcctctgtc atggttacaa
     7261 acaccagcac aggtatttct tcccagcaga ctacggacaa caaaggatat tacatctttc
     7321 cgcaactggc cattggcggg ccatatacgg tcagtatcac ggcgacaggc ttccaatcct
     7381 ttaagacggt ggggcttgtc ctgcaactga acgaaaaccg gcaggtagat gcagtacttc
     7441 aaccaggggc agtggcgcag accatcgagg ttacggcaaa tgcgatccag gtggagactt
     7501 ctgatacgca gttgaagacc acagtcacat ccaatcagat tgagcagttg ccgcttctgg
     7561 gacgcgaccc aacggccttg cagaaactga caccgggcac cgtggaatct tcagataggt
     7621 ttggcaattt ttcggcaaac ggttcacaga cgcaggaaaa tgcttatttg ctggacgggg
     7681 ctgatattaa cgatactcct ctccagacca acagcgtacc cattaaccct gatgccatcg
     7741 gggaaaccac ctttgtgact ggcactcaaa acccggaata tagccgtaac tctggcgcca
     7801 tcatcaatca gacgctaaag agcggcacca acagttttca cggagatgct tttgaatttt
     7861 atcgcgacac atttttaaac aacggaaatt atttttcgac gacccgcccc gtttttcacc
     7921 agaacatttt tggcgggacg ctgggtggcc cggtgattaa gaacaggctg ttcttcttcc
     7981 ttgcgtatca gggactgaga aattcaactg ctacaaccac ggttacgcct gtgccgacag
     8041 acgcacagtt ggggcgcaat agtacaggtt ttgccgattt gacgaatgac aacaacgtgg
     8101 ctaacagcgg cccgaacact tcagtcgggc tgaccagcaa tcctttgccc ttcacaattc
     8161 agggacccgg aggaacacct tgtcccgctg gtacgccatg gaaccagtgc tttcctggac
     8221 caacctatca actgccggtt tcagacttta attccatatc ttcgaatctg ctggcaaagt
     8281 atgtgccttc tcccaatttc aacggcaagt tttataactt caatgcagcg aatacagccg
     8341 gcgcagacca gggcatcatt cgcattgaca caaaggtgac tgccaacgat cagctctggg
     8401 catcaagcat tttccaatcg aatccaagtt tgaatgcgtt gccatttacc ggatccacgc
     8461 tgcccggttt tgctcaggat gcatcccggc acttcaaaat atttagcgcc tcgtggacgc
     8521 actcttttaa tccaactact ctgaacgaat tgcgagcagg atattac
//
LOCUS       DASRBV010000237         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_473660, whole genome shotgun sequence.
ACCESSION   DASRBV010000237 DASRBV010000000
VERSION     DASRBV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473660"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFA02_08890"
     CDS             <1..832
                     /locus_tag="VFA02_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10010.1"
                     /translation="IGPALGVLVGALLLDKFGWRMMFAITGLGALIWVPCWLLAAPPD
                     SVRAERPAELGIEDPEVSRPWTWRMLLANETFWAMALCILLSSYYWYFVLTWVPSYLI
                     MSRGFSTLGMGRVLSTALFTMAAVNVIAGAAADRLAARIGVFRARLWFGVLGYVGTGA
                     ILLLLVTNRSWSLPILAFSLCATGIGNSTYWTIIQHASPEKMVGRSVGFLNTVSVAAG
                     AVAPIVTGWILGPQKHFGPAIIVAGVCPVLAALCLLVAGSKGLAKIKSLLAGEAYSEN
                     "
     gene            1118..1423
                     /locus_tag="VFA02_08895"
     CDS             1118..1423
                     /locus_tag="VFA02_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10011.1"
                     /translation="MRTLFKYAVPALLIYAALLGGMYAAMLQPPAVFGRIMSKLPMVT
                     YFLFPFEPMWLIARRGHLRLGDPAPDFVLQTADNSQAVRLSSFRGRKPVVLIFGSHT"
     gene            1436..1828
                     /locus_tag="VFA02_08900"
                     /pseudo
     CDS             1436..1828
                     /locus_tag="VFA02_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
BASE COUNT          385 a          589 c          636 g          499 t
ORIGIN      
        1 catcggccct gcgcttgggg tgctggttgg agcgctcctg ctggacaagt ttggatggcg
       61 catgatgttt gccatcacgg ggctgggtgc gctgatctgg gttccctgct ggctattggc
      121 cgctccgcct gacagcgtgc gcgctgagcg tccggctgaa ctcggcatcg aggaccccga
      181 ggtatcgcgt ccctggacct ggcgcatgct gctggccaac gagacattct gggcgatggc
      241 gttgtgcatc ctgctctcgt cttattactg gtattttgtt ctcacctggg tgccgagcta
      301 tttgatcatg tcgcgaggct tctcaacgct tggaatgggg cgtgtgcttt ccaccgcgct
      361 ctttacgatg gccgctgtca atgtcattgc aggtgcagca gcggacaggc tggctgcacg
      421 aatcggcgtc tttcgcgcgc gtttgtggtt tggcgtcctg ggatatgtgg gaaccggagc
      481 cattttgctg cttttggtga caaaccgctc atggtcgctt ccgatacttg ctttttcgct
      541 gtgtgcgacg gggattggga attccacgta ttggacgatt attcagcacg cctcgcccga
      601 gaagatggtt gggcggtcgg tggggttcct gaacacggtt tccgtggccg caggcgcagt
      661 cgctcccatt gtcacgggct ggattctcgg accgcaaaag cacttcggcc cggcgatcat
      721 cgttgccggc gtgtgtcccg ttctggccgc cctctgcctc ctggtggccg gatcgaaagg
      781 tctcgcaaag ataaaatcgc ttctggcagg agaagcatac tcggagaact gagcgcaaag
      841 atgtccaaca gactgcctcg ccctgaaagc catgttcacc gataaaattg gctgctatta
      901 cccagtttta ttcgcttcca caccagcttc aggttattga ccgcccgcag cgctccgttt
      961 agcctatgca ggactttggg gtaatttgag tcgggcccgg ggtgccatca ctaaagcttg
     1021 atcattgaag cggcgggcac gttcgtctcc tcgcgggggt atgtttctgc ccgccgctgc
     1081 ggccgcggtc tcgggagcag ggggcgtgcg taatcatatg aggactttat tcaaatatgc
     1141 tgttccggcg ctgttgattt atgctgcgct gctcggcgga atgtacgccg cgatgctcca
     1201 acctcctgca gttttcggcc gcatcatgtc gaagctgccg atggtgacct attttctttt
     1261 cccatttgag ccgatgtggc tgatcgcgcg caggggtcat ttaaggctcg gcgacccggc
     1321 gccggatttc gtgctccaga cagcagacaa ctcgcaagct gtgcgcctgt cttcattccg
     1381 ggggcgcaaa cctgttgttc tgatcttcgg cagccatacc tgaccgccgt ttcgtcggga
     1441 ggttcccgcg ctcaacaaac tatatgagca atacaagggc cgggccgcat tttacgttgt
     1501 ttacattgag gaggcgcacc ccaccgatgg gtggcagatg ccgagcaaca ttaaggaaaa
     1561 catcctcatc gccagcgcca agaccttctt ccagcgtgat gaagccgcca aaacatgcgt
     1621 ggccaaactt ggcattcaca ttcccgccct ggtggacgat gtgcaggatt ccacagagcg
     1681 cgcgtatacg gcgtggccgg acaggctcta cgtcattgac cgcgaaggcc gagtggctta
     1741 caagagcaag gccggaccgt ttggcttcaa tcccgcagag atggccgcgg cccttcagcc
     1801 gctagtcgct gctgatggca aacaatgagt taaaaacggc attcctggta acccacaggc
     1861 ccggatgcca gaacggcggc tgcgctttct tgagccagtc gctgggcctg tcaaagaaat
     1921 cggaaaggtc ggaagcaaat gaaagaaagc atgtccaaag ttgtttcaag ctgtttcttt
     1981 ctccttttgc ccgcgctgat gtgcagccct ttggctgcac aggaggttac ggcgagcatc
     2041 cgaggtgtag tgcttgaccc gagtggcgcg ggaatcccgg ccgcggaagt gacggcgacg
     2101 cagtctgaa
//
LOCUS       DASRBV010000238         6384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_369598, whole genome shotgun sequence.
ACCESSION   DASRBV010000238 DASRBV010000000
VERSION     DASRBV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369598"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFA02_08905"
     CDS             <1..1131
                     /locus_tag="VFA02_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10012.1"
                     /translation="LLGTISEQPDFLVSPEVLPYIFSLRGDRNWKRGPGNKSSQLAAD
                     IWKLLKRENSLTLLELQDKLGRGVSEAAVHRALTELWTTLYVTPVYVEHAPATWTLFE
                     SRYQSAMQAGSGMGQSVAISAMISLYLDSVIAATPEETETFLSPLAPRSRIREVARGL
                     LAMRQLSTMPVGPQSLLFITGGLPEFPAAEVPAETPAPAPAAEERPQQPRQPRKPFAP
                     RPARRPPGERYPQRERREEGRPRRSHTGGERRPHPEGERREWKPRRREDQGGFKTSRR
                     EGGEWKSRSGQGGESRPFRREKRPFFGEKRQSEQGRPARRDFSREQDSTEGRKPGRSS
                     KPQGRYHPKSGAKSGPKPGRRGPKPGGKFVPRRPRRKDEPGE"
     gene            1128..1808
                     /gene="cmk"
                     /locus_tag="VFA02_08910"
     CDS             1128..1808
                     /gene="cmk"
                     /locus_tag="VFA02_08910"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZU10013.1"
                     /translation="MSAERCPLIIAIDGPAGAGKSTVATRLASRFGLLNLETGAMYRA
                     FALKALEQHLDLSNADALEQLAASTTIHLEPTASGNRVLLDGADVTNRIRQSDVTQAA
                     SQVSVHPAIRRWMVDLQRELGANGGIVMEGRDIGTVVFPHADVKIFLDASPEARSERR
                     YAQTGGAVSQEAVLREIRERDERDRTREQSPLKPAPDAVVIDSTDLALDEVVGRIEAL
                     IIARLGAR"
     gene            complement(1783..3330)
                     /locus_tag="VFA02_08915"
     CDS             complement(1783..3330)
                     /locus_tag="VFA02_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZU10014.1"
                     /translation="MAAAPPPYNSRDIRQQWKDYARAQKQAARARSFYWRYYHRPSIA
                     GPLLLLSIGILALLIETGRVSAARFWVWYAQWWPVILIGIGAVLLIEYFLDRNNPYAG
                     RRTFGGFGWIIVLVLSTAWGTHAAHVWGPLSDQFSDNGDDFWSMMGEEHDNDVELSQA
                     VSPTAAVQIQNPRGDVTITASSDNQAHVRAHQVVHTSSEDEANKVFNAVKPKLIVSGS
                     SAVLSVEGRNNARVDLTVEVPAGVTTVVNAGHGDVALDGLKGTADVTSSHGDVKFDNM
                     GGNVHARMDHGDFSAHQITGDVAVDGHAGDVTLSEIKGNITLDGDFFGDTHVEQAGSA
                     VHFHSSRTDLAITKLVGDMTMDSSDLSITQAIGPLRVVTRSKNIDLSQITGDAHVENN
                     NGDITLTAAVPLGNIQITNRTGNISVTVPENAGFSVTASNNDGDLNTDFPISVTSSDG
                     HKTAQGQVGNGGPHLELTTSHGDIQLRKGGTAQPVVPAPPKPPEPPVPPKRLHAPRGA
                     SEHPTEQ"
     gene            complement(3334..3624)
                     /locus_tag="VFA02_08920"
     CDS             complement(3334..3624)
                     /locus_tag="VFA02_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10015.1"
                     /translation="MNQYFFLQRITGPAMILTFGITALLDEWHILGYGKSWPLYLIVL
                     GVIKLAQRAALLNAEPPAYPPPYAPPAAPPPPGPGPAAVNAAPPENHSGERS"
     gene            complement(3621..4463)
                     /locus_tag="VFA02_08925"
     CDS             complement(3621..4463)
                     /locus_tag="VFA02_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HZU10016.1"
                     /translation="MNCTNHPETPVAAYCQNCGKPLCSECVRSVSGVIYCEQCLAAKL
                     GIGAASGVAFHFSGTSGGTNYSFSGVTPPPEPDRPNPGLAALLGFIPGVGAMYNGQFI
                     KALVHVLVFVVLVGIVGEHGIFGIFIAAWVFYQVFDAYQTAKARRDGLPLPDPFGLNE
                     LGQKMGIPNRPWPPVAGAAPAAGPVPPSVPPTVPPVPPPPGYEPVPPVPPVPPDPACC
                     GPFGRRPEPIGAIVLIIVGMLFLFSTLGFLRFDWIGHGWPIIVIAVGAWLLIRHMRES
                     GGAQ"
     gene            complement(4460..5386)
                     /locus_tag="VFA02_08930"
     CDS             complement(4460..5386)
                     /locus_tag="VFA02_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZU10017.1"
                     /translation="MSERNPFRQNSGGPLDCETWEALLADALDGTLSARDQAAFTAHS
                     QDCAICAEMLAQAKQGQEWMRFLHEEPDAPGDLISKILGRTSGATLPQLAVAGGGVQP
                     IAPHIAQAAMRRAFHDSRLLMTVAMAFFSIALTLNMLGVNLKGVRLADLKPSALQTTI
                     ARQIYGAKKQMVSYYENLRWVYELDSRLRELRRDAETEQQPQKEQKNDKQPHKNGGKV
                     TTPKEAAPSTGILQGKEVMASVDMRSKGKAGPSATPQNPNSTVHPQIKSVLMVLNDCS
                     RKYEVRYAAHQKSEVNELAVRFFAAQAERSLA"
     gene            complement(5387..6127)
                     /locus_tag="VFA02_08935"
     CDS             complement(5387..6127)
                     /locus_tag="VFA02_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU10018.1"
                     /translation="MSAAGTITWSGWAARPVAVRREPIGPWNAAANQAHRLNDKVGER
                     HLEADWQMVVRRCLDGDSLAWTELVKAHHRRVYALCYRFTGSPHDAEDLTQEVFLKIY
                     GNLATFDLAKGSFPTWITTLTRNLLVDHFRRTKQQRVTDSMDAGWDGNGSEEHSASPL
                     SDRISDPGPTPHEQAAKKELEKMVQQALTKVSPELREAVILRDLQDMDYKEIAQVLRI
                     PEGTVKSRISRGRAELARLLERSGRQVV"
BASE COUNT         1331 a         1856 c         1890 g         1307 t
ORIGIN      
        1 cttctgggaa cgatttcaga gcagcctgat tttctcgttt cacctgaggt cttgccttat
       61 atcttttccc tgcgcggaga ccgcaactgg aaacgtggac cggggaacaa aagctcacaa
      121 ctggcagccg acatctggaa gctgctgaag cgggaaaact cgctcactct cctggaacta
      181 caggacaagc tggggcgcgg ggtaagcgag gccgctgttc atcgtgctct gaccgagctg
      241 tggacaacgc tttatgtgac gcctgtttat gttgaacatg caccggccac atggacgctc
      301 tttgagtcac gttaccagag cgccatgcag gcagggagcg gaatggggca gtcggtcgcg
      361 atttcggcca tgatctcgct ctaccttgat tccgtcattg cggcgacacc ggaagaaact
      421 gagacatttc tttcaccgct tgcgccccgc tcacgcattc gggaagtggc ccgcgggctg
      481 cttgccatgc gtcagttaag caccatgcct gtgggtccgc agtctctgct cttcataacc
      541 ggcgggcttc ccgagtttcc tgctgcggaa gtacccgccg aaactcctgc gcccgctccg
      601 gctgcggaag agcgcccaca acagccgagg cagccgcgca agccgtttgc tccccggccg
      661 gcgcggcgtc cgccgggtga acgttatccg caaagagagc gaagagaaga aggccgtccg
      721 cggcgctcgc atacaggcgg ggaacgcagg ccccatccgg aaggcgaacg cagagaatgg
      781 aagccgcggc gccgggagga ccagggcgga ttcaagacct cacgccggga aggcggagaa
      841 tggaaatcac gctccggaca aggcggtgag tcacgtccgt tccgacggga gaagcggccg
      901 ttctttggcg agaaacggca aagcgaacag gggcgcccag cacgcagaga tttttctcgc
      961 gaacaagaca gcaccgaggg ccgcaaacca ggccggtcct cgaagccgca aggcagatat
     1021 cacccgaaat ccggtgcaaa atcaggtcca aagccagggc ggagagggcc gaaaccgggc
     1081 ggaaaatttg tgcccagaag accgagaaga aaggacgaac cgggcgaatg agcgccgaac
     1141 gttgtccact cattatcgcc attgacggtc cggcaggagc aggcaagagc acggtagcta
     1201 cccgcctggc ttcgcgcttc gggcttctga atcttgagac gggggccatg tatcgcgcct
     1261 ttgctctgaa ggcacttgaa cagcaccttg atctaagcaa tgcagacgct ctcgaacagc
     1321 ttgctgccag caccaccatt catctggagc caacggcctc cgggaaccgt gttctgctcg
     1381 atggggccga cgttacgaac cggatccgcc agtcagacgt gacccaggca gcatcccagg
     1441 tgagtgtgca tccggccatc cgccgctgga tggtagacct ccagcgcgaa cttggcgcaa
     1501 acggtggaat cgtaatggaa ggccgggaca ttggcacggt cgtctttcct catgctgatg
     1561 tcaagatttt tctcgacgcc tcacccgagg cccgcagcga acgccgctac gcgcagaccg
     1621 gaggcgcagt ctcgcaggaa gctgtgctca gggaaattcg tgagcgtgac gagcgcgacc
     1681 gcacgcggga gcaatctcct ctgaaacctg cgccggatgc ggttgtgatt gattccactg
     1741 atctggctct ggatgaggtc gtcggcagga tcgaagcact gatcattgct cggttgggtg
     1801 ctcgctagca ccgcgcgggg catgaagacg ctttggagga accggaggtt ccggcggctt
     1861 tggaggagct ggcacaaccg gctgtgctgt gcctcctttc cgcagttgga tgtcaccgtg
     1921 gctggtggtc agctccagat gcgggccgcc attgcccacc tgcccctggg ccgtcttgtg
     1981 gccgtccgaa ctggtcacgc tgatggggaa gtccgtattc aggtcaccgt cattattgga
     2041 agctgttacg gaaaaacctg cattttccgg cacagtgact gaaatgtttc ctgtccggtt
     2101 ggtgatctga atattgccca gaggaaccgc tgcggtcaac gtaatgtcgc cgttattgtt
     2161 ttcgacgtgc gcatctcccg tgatctgcga aaggtcaata tttttcgagc gtgtcactac
     2221 gcgcagcggg ccaatggcct gagtaatgct caggtcgctg gaatccattg tcatgtcgcc
     2281 cactaatttc gtaatggcaa ggtcggtgcg gctggaatga aaatgcacgg cagaaccggc
     2341 ctgttccacg tgcgtgtcgc caaagaaatc gccatccaga gtgatgttgc ctttaatctc
     2401 agaaagcgtc acatcgccgg cgtgtccatc cacggccaca tcccctgtaa tctggtgggc
     2461 ggaaaaatcg ccgtggtcca tgcgggcatg gacgtttccg cccatgttgt caaatttcac
     2521 gtcgccgtgg ctggaagtca catcggcggt acctttcagt ccgtccaacg ccacatcgcc
     2581 atgcccggca tttaccaccg tggtcacgcc ggcagggact tctactgtga gatccacgcg
     2641 cgcattgtta cggccttcta cgctcagcac agcgctcgat ccggaaacga tcagcttggg
     2701 ctttaccgca ttaaagacct tatttgcctc atcctcggaa gaagtatgga caacctggtg
     2761 cgcccgcaca tgtgcctgat tatcactgga tgcggtgatg gtgacatcgc cgcgcgggtt
     2821 ctgtatctgg acagcagccg tgggtgaaac agcctggctc agctcgacat cattgtcgtg
     2881 ctcctcgccc atcatgctcc agaaatcgtc gccattgtca gagaactggt cactcagcgg
     2941 tccccagacg tgcgctgcgt gcgttcccca ggcggtggag aggaccagca caatgatcca
     3001 gccaaagccg ccaaaggtcc tgcgcccggc ataaggattg ttgcggtcca gaaagtattc
     3061 aatcaacaga acagcgccaa ttccgatgag gatgaccggc caccactgtg cataccagac
     3121 ccagaatcgc gccgcgctca ctcttccggt ctcaatcagc agggcaagaa tgcctattga
     3181 aagcagaagc agcgggcctg caatcgaagg ccggtgatag tagcgccagt agaagctgcg
     3241 tgcgcgcgcg gcctgcttct gtgcccgcgc atagtctttc cactgctgtc ttatatcgcg
     3301 tgaattgtag ggcggaggcg ctgctgccat ggcttagctc ctctctccgg agtggttttc
     3361 cggcggtgcc gcattgaccg ccgctggtcc gggaccagga ggaggaggag cagccggcgg
     3421 agcataggga ggcggatagg ccggcggttc tgcattgagc aaagcggccc tttgcgccag
     3481 cttgatgacc ccgagaacaa tcaggtaaag cggccagctt tttccatagc ccagaatgtg
     3541 ccactcatcg agcaatgctg tgatgccaaa ggtgaggatc atggccggcc cggtaatgcg
     3601 ctgtaaaaaa aagtactggt tcattgcgcg cctccgcttt cccgcatgtg gcgaatcagc
     3661 agccatgcgc caaccgcgat gacgataatc ggccatccat gcccgatcca gtcaaagcgg
     3721 aggaagccca gtgtgctgaa caggaagagc atgcctacga taatcagcac aatggcgcca
     3781 ataggctcag gccggcgccc aaatgggccg caacaggccg ggtcaggagg aacaggaggc
     3841 accggtggaa ccggctcgta tcctggcgga ggaggtacag gcggtacagt tggcggcaca
     3901 gagggcggaa cggggcctgc cgcaggcgca gcgccggcaa ccggaggcca cggccggttc
     3961 gggatgccca ttttctgtcc gagttcattc agtccgaaag gatcgggcag gggaagcccg
     4021 tcgcggcgcg cttttgcggt ctggtaggcg tcaaacacct gataaaagac ccaggcagca
     4081 atgaagatac caaagatacc atgctcgcca acgataccaa ccagcacaac aaaaaccagt
     4141 acatgaacca gcgccttgat gaactgtccg ttgtacatgg cgccgactcc ggggataaac
     4201 ccgagcaggg ccgccaggcc ggggttgggt ctgtccggct ccggcggagg agtgacgccg
     4261 ctaaaactgt agttggtccc gccggacgtc ccggaaaagt ggaaggcaac gccgctggct
     4321 gctccaatgc caagtttcgc cgcaaggcac tgttcgcaat aaatcacgcc ggagacagag
     4381 cgcacacatt cagagcaaag cggtttgccg cagttctggc agtaggccgc aaccggagtt
     4441 tcaggatggt tcgtgcagtt catgccaggc tcctttctgc ctgggctgca aaaaatctga
     4501 cagccaattc atttacctct gatttttgat gagcagcgta ccggacctcg tactttcgag
     4561 aacaatcatt cagaaccatc agtactgatt tgatttgggg atgaacagtt gagttgggat
     4621 tctgaggtgt agccgaagga cctgcttttc cttttgaacg catatccaca ctcgccatca
     4681 cctctttgcc ctgcaatatg ccggtcgaag gagcagcttc cttcggagtc gtcacttttc
     4741 cgccattctt atggggttgc ttgtcgttct tctgctcttt ttgcggctgc tgttcggttt
     4801 ccgcgtcgcg gcgcagctcg cgcagtctgg aatcaagctc atatacccag cgcaggttct
     4861 cgtagtaact caccatctgc ttttttgcgc cgtaaatctg gcgggcgatg gtggtttgca
     4921 gcgcggaggg cttcaggtca gcaaggcgca cgccctttag attcacaccc agcatgttca
     4981 gcgtcagcgc aatcgagaaa aatgccatcg cgacggtcat cagcagacgc gaatcatgga
     5041 atgcgcgccg catggcggcc tgggcaatgt gcggcgcgat gggctgcacg ccaccgccgg
     5101 ccactgcaag ctggggaaga gttgcgccgc tggtgcggcc aagaattttc gatatcaggt
     5161 cacccggggc gtccggttcc tcatgcagga accgcatcca ctcctgaccc tgctttgcct
     5221 gagccagcat ttctgcgcag atggcgcagt cctgactgtg ggcggtaaaa gctgcctggt
     5281 cccgggccga gagtgtgccg tcgagagcat cggccagcag cgcctcccat gtttcgcaat
     5341 cgagcgggcc tcccgagttt tgccggaatg gattacgctc agacatttaa accacctgcc
     5401 ttccactacg ttccagaaga cgcgccagtt ccgcccttcc gcggctgata cgcgacttca
     5461 cggtcccttc aggaatacgt aaaacctgcg cgatttcttt gtagtccatg tcctgcagat
     5521 cgcgcagaat gactgcttcg cgcagctcgg gcgagacttt ggtcaacgcc tgctgcacca
     5581 ttttttctaa ttcttttttg gccgcctgct catgcggcgt gggcccgggg tcggaaatcc
     5641 ggtccgataa cggactggcg gagtgctcct cgctcccgtt gccatcccag cccgcatcca
     5701 tcgaatctgt tacgcgctgc tgtttggtac ggcggaaatg gtccaccaga agattcctgg
     5761 tcagagtcgt gatccaggta ggaaagctgc ctttggccag atcaaatgtc gccaggtttc
     5821 cgtagatttt cagaaagact tcctgggtga ggtcttcggc gtcatggggc gacccggtaa
     5881 aacgatagca aagcgcataa acgcggcggt ggtgtgcttt gacgagttcc gtccatgcca
     5941 gcgaatcgcc atcgaggcac cgccgcacca ccatttgcca atccgcttcc aaatgcctct
     6001 ccccgacctt gtcgttcagg cggtgcgcct ggtttgcagc cgcattccac ggacctatgg
     6061 gctcccgcct tacggccaca ggtctcgctg cccatccact ccacgttatg gtgccggctg
     6121 cgctcatgca acagaatacg caatcctgct ggcggcagtt ccgttttttg ggccattccg
     6181 ggctgcggga tgaaggagat gaaggctttt ccgggggtac tgacagcgat ggtttcaagg
     6241 aaaacgtctt ccatgaaagc gctgattccg gcgggccgcc ggccattttg gcaagcggtg
     6301 gtctgttttc aggggaaata taaaaatgct tgacaagtgg tttcctggcc ccatatagtt
     6361 ggctccgatt ggaaacgata ccag
//
LOCUS       DASRBV010000239         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_420350, whole genome shotgun sequence.
ACCESSION   DASRBV010000239 DASRBV010000000
VERSION     DASRBV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420350"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..602)
                     /gene="ruvX"
                     /locus_tag="VFA02_08940"
     CDS             complement(123..602)
                     /gene="ruvX"
                     /locus_tag="VFA02_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZU10019.1"
                     /translation="MENVARILGLDVGNRRIGVAISDELGITAQPVLTLLRKNPRADV
                     RSLARLVRKYNCREIVVGNPLRLTGGKSPQAEKTQKFAQWLADETKLPVHLWDERLTT
                     VEAHRILYQAGRHRTEHRRVVDQVAAVLILQDFLDSRGAPANATYHPESGPPSSSQV"
     gene            791..1777
                     /gene="mltG"
                     /locus_tag="VFA02_08945"
     CDS             791..1777
                     /gene="mltG"
                     /locus_tag="VFA02_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZU10020.1"
                     /translation="MRRLLLLAVIAVLLFCGFSIYVPRGPRSEMFLEVPPHTGSNQIA
                     ALLQSHRLIWNRYVFDAMRLVKGGTLKAGEYRFDHPVRMAEVYARLEKGDVYTVAFTI
                     PEGANIFDIAQRVENARLGSRDAFLEAAHKDVALISDMEPDAQTVEGYLFPDTYHFSR
                     HVTPDQMIAAMVKRFRQAADSIDLVGNYHSVVTLASLVERETPIQSERPVVASVFVNR
                     MNKGMPLMTDPSVIYADLLEGRYRGTIFRSDLDGDSPYNTYKHTGLPPGPICNPGIVS
                     LQAAMHPAKTEYLYFVAASADPSGNSRFAKTLEEHEKNVQAYRRSQRAAGQR"
     gene            1912..>2474
                     /locus_tag="VFA02_08950"
     CDS             1912..>2474
                     /locus_tag="VFA02_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10021.1"
                     /translation="MRKLWIHAFAMCLLPALTGCLIHTRKVPQPKMPSVVLSADAQQL
                     VSNINQQYDSIQTLNATVEFQATVGGEKKGKITDYTSIRGYILLRKPEMLRVLGMLPV
                     ISTRAFDLASDGNTFKLWVPHENKAIVGTNTVTKPSPNALENMRPYIFFDSLLIHSIG
                     PDDLLSLTTDTRTITDPKRKQLLLQPE"
BASE COUNT          571 a          674 c          673 g          556 t
ORIGIN      
        1 agctgcttga tctcttcgag cagttttttc ttgatgtgtt caggcatggg gaataatccg
       61 tcgcggccca taggcatggg ccgttccgag cacggccaaa ccagaaaacc gctgaatctt
      121 tattatacct gcgaggacga ggggggccct gattcaggat gataagtagc atttgccggc
      181 gcccccctcg aatccagaaa atcctgcagg atcaggacgg cggccacctg gtccaccact
      241 ctccggtgtt cggtgcggtg acggcctgcc tgatacaaaa tgcggtgcgc ctcgacggtc
      301 gtcaggcgct cgtcccacaa gtgcaccggg agctttgtct catcggccag ccattgcgca
      361 aatttctgcg tcttttcggc ctgcggactt ttccctccgg tcaggcgcag tgggttgccg
      421 acgacgatct cccggcagtt atatttgcgg accagccggg ccagagagcg cacgtcggcg
      481 cgcggatttt tgcgcagcag cgtcaacacc ggctgcgcgg tgatgcccag ctcgtccgag
      541 atagccacgc caatccggcg attgccgaca tccagcccca gaatgcgcgc gacattttcc
      601 ataagagcat cttacgcagg tccgttgcgt atggtggtgc gttgaagccg gcgtggccga
      661 agaattttcc ggaaaaggca cggagagcga cgggaggact ttccagcccg ctccggagac
      721 aagccaaaaa aaatagtcgc atcatacaat tcaggtaggg aatcaaaaca acttataggt
      781 ctttccggaa ttgcgacgtt tattacttct ggcagtcatt gcggtgctgc tgttctgtgg
      841 tttttccatt tatgtgcctc gcggtccccg gtcggagatg tttctggagg tgccgccgca
      901 tacaggatca aaccagatag ccgccctttt gcagtcccat aggctgatct ggaaccggta
      961 tgtctttgac gcaatgcgcc tggtgaaggg cggaacgctc aaggcaggcg aataccgctt
     1021 tgaccacccc gtccggatgg ccgaggtcta tgcgcgtctg gaaaaaggag acgtctacac
     1081 agttgcattc actattccgg agggggccaa tatttttgat attgcccagc gggtggaaaa
     1141 cgccaggctc ggcagcagag atgcttttct ggaggcggca cacaaggacg tggcattgat
     1201 ttctgatatg gagccagatg ctcaaactgt agaaggctat cttttcccgg acacttacca
     1261 tttttcccgc cacgttactc ccgaccagat gattgcagct atggtgaagc gcttccggca
     1321 ggcggctgac agtattgatc tcgtgggaaa ttaccactcg gtcgtaacgc tggcttcgct
     1381 ggtggaaagg gaaacgccca tccagagtga gcggcctgtt gtagcaagtg tcttcgtcaa
     1441 ccgcatgaat aaggggatgc ctttaatgac agacccttcc gtgatttatg ccgatttatt
     1501 ggaaggaaga tacagaggaa ccatttttcg ttctgatctg gacggagatt cgccctacaa
     1561 cacgtataaa catacagggc tgcctccggg accgatctgc aatccgggca ttgtgtcact
     1621 gcaggcggcc atgcacccgg ccaaaacaga gtatttgtat tttgtggcag caagcgctga
     1681 cccttcagga aattcccgtt ttgcaaaaac gctcgaagaa cacgagaaga atgttcaggc
     1741 gtacagacgc tctcagcgcg cggcaggaca acgttgaaag ggtacagctc ttattgaatg
     1801 ccgaatagca gtcttttcat gctgtttcgg ttcgggggag cagggcagat atttttctgc
     1861 tccaggttcg tcaaatcaca attacaggtc gtatatacat tcagggccag gatgagaaaa
     1921 ctctggattc acgcatttgc gatgtgtctg ctgccggcac tcaccggatg cctgattcac
     1981 acccgtaagg tcccccagcc gaaaatgccc agcgtcgtcc tctcagcaga cgcgcagcag
     2041 cttgtcagca atatcaatca gcaatatgac tccatccaga cgctgaatgc gaccgtagaa
     2101 ttccaggcca cggtgggcgg tgaaaagaaa ggaaaaatca ccgactacac ctcgattcgt
     2161 ggctacattc tgctgcgcaa gccggagatg ctgcgcgttt tgggaatgct tccggtcatc
     2221 agcacacgcg cctttgatct ggccagcgac ggcaacacgt tcaagctctg ggtcccgcat
     2281 gaaaacaagg ccattgtcgg cacaaacacg gtcacaaagc cttcgcccaa tgcactggaa
     2341 aacatgcggc cgtatatctt ctttgattcg ctgctgattc acagcatcgg accggatgat
     2401 ctgctctcgc ttacgacaga caccaggacc atcactgatc cgaaacgcaa gcaacttctg
     2461 ctccagccgg aata
//
LOCUS       DASRBV010000240        12936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_680556, whole genome shotgun sequence.
ACCESSION   DASRBV010000240 DASRBV010000000
VERSION     DASRBV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680556"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="VFA02_08955"
     CDS             complement(<1..2090)
                     /locus_tag="VFA02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-lysosomal glucosylceramidase"
                     /protein_id="HZU10022.1"
                     /translation="MPNLIDDGYWQGAPVGGFGAGTFSRSYRGNFERWHIKAGVHKYE
                     DVPGNQFAVFVQPEGETGRAIALSTGKPLNGALSSWNWSYPAGAGEYAALYPKSWFAY
                     DPKELGIKLTVEQFSPVLPNNYKESSYPVALYNWYAENPSDKPVTVSLLFSWTNMVGW
                     FRDVSSGFSGALSNQDKNRYVSEDVPGATIQGILFDRIRSGAVRSEWDGQFAIATKTS
                     PDMEVTYLTTFYPQGPGMEVWEPFADDGRLPNAAPDVASSGEQIAGAIAVRFTLAPGE
                     KKMVPMALSWDLPLIQFGGGRKWVRHYTKFFGAEGTHAWQIAKTALAEDENWSHAIDA
                     WQKPYIEDESLPEWYRGELFNEMYILADGGTVWAHELDGIGNPAHAATKDEDTFSFLE
                     CFDYVFYGTLDVRFYGSFPLIRFWPDIEKQIMREYTDTIQESNPQKYLWAWQAQHEHK
                     FVTMERKVAGAAPHDLGAPQEDPFVNVNQYNYQDVSNWRDLNSKYVLLLWRDYVLTGS
                     KDLAFLQYSWNSVQRAMEHLQQYDTDGDGLIENGGFPDQTYDDWIASGESAYSGGLYL
                     AALRATAQIAQRVGDPGSAAQYNALFQKAQTAYIRKLWNGTYFDYDTNSEYHSDIMAE
                     QLAGQWYANLTGLGDLVPKKMRLSALKRIFNYNVMLFGNGEMGAVNGIGANGEILTTN
                     PQVGEVWTGTTFGVA"
     gene            2421..2903
                     /locus_tag="VFA02_08960"
     CDS             2421..2903
                     /locus_tag="VFA02_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HZU10023.1"
                     /translation="MMTGCGYHTVHSAAHLPDTVHTLAVPTFANHTQSFHTEVSFTNA
                     VIREFTSRTSYKVLATDKPGAGDAVVDGAINSFQVVPLTYNIQTGQSSSYLITIHASV
                     KVLDRNKRVLYENTHYTFRQQYQTTQDLVSFIQEDPAAVQRLSRDFAQALVSDILESF
                     "
     gene            2905..4041
                     /gene="holA"
                     /locus_tag="VFA02_08965"
     CDS             2905..4041
                     /gene="holA"
                     /locus_tag="VFA02_08965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZU10024.1"
                     /translation="MGAGFASTDRFLAEIRGNKLRAGYVLIGDEIFLYERCRRALLDA
                     FVPSDLRDFCLSEIDLGETSIFEVLDRAQTPSLMAPFQVLFVRNLKQLYTRGAKKDEF
                     AAIDAYFRSPNPQAVLIFVADHIRIPSDPRRMDMGDKDRYERIRETLGDHCGMVELAR
                     VDEADALRWLLAEAEKQQIRVEQDAARELVDALGADMMLIANEFEKLTLYVGLKKHIT
                     LGDVETMVLAAKQRSLYELTDAISARDKKRALALLEGLLNASDGGEESAIGHLYMLAR
                     TFRQMLVILEKNVRDSRAIWQALWQGFRMPPFAAEDLIRQARRYKSKRELTRALRLIA
                     RADLELRSQPPDKKLVLERLVMDLASEPRTTQAGAASMQYAMEI"
     gene            complement(4187..6103)
                     /locus_tag="VFA02_08970"
     CDS             complement(4187..6103)
                     /locus_tag="VFA02_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10025.1"
                     /translation="MQFKSPTFARSFLIVLLVFAALPFSAQQGNAPLVTDDQILSHLN
                     AVITWYRNVKTQVEPAGLPTDALYQANAESIAAKAVRYAFESAEKAAPLVPNENNEAA
                     SPTSRQQLLKFLDDTKAHNADLEKQIAQLNDRIAAAPGRNAQPLLDQRDRLQGELDLG
                     QAMLNSLMQLTQTTNQVSTNTKQNQKSKQQAQSKNQVSPNGFAASVAQLKSSVPEVFN
                     GKPSTPSSTPQIASANGLIGQLRRLYSQSMTLHEIDTLTDQTAKLEDLVNAMRTPLRS
                     AMKETVQQGRALSAAADNPQPGQTPPGKKDFDDLATKFNQLAAVEIPLGQEASALEES
                     KANLLDWRASIASQYGSILRSIFITVAILIAVLGILLLVSNLWKRAIFRYITDVRRRR
                     QFLVVRRFVIGFLIGLVLVFSVISEFSSLATFAGFITAGLAVGLQTVLLCVAAYFFLI
                     GRYGIRVGDRISIAGVTGDVVDVGFVRFYLLELAGTGADLHPTGRIVAFANSVLFQAT
                     TPMFRQFPGTAYTWHEVAVSLQPSANYELVESNMLEVVNNAYKKYRDALDRQHGNLEM
                     RFELPLTPLKPTAQLQLTGTGLEAVVRYPVSLRNTADTDDEITRSLIEKISKDPEMSA
                     AVQGFPRIRSVIRN"
     gene            6239..6799
                     /locus_tag="VFA02_08975"
     CDS             6239..6799
                     /locus_tag="VFA02_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10026.1"
                     /translation="MKDPGWTRTVSVAAGILLTLSACSSGRNKPTPENFKAALNSYFT
                     NRSECLFPSAPQFPYEVSPSPTAKAEQKRMDALMAAALVKRKADESIHVYIYSLTPAG
                     ERATSRFCYGHRQVTSIDNFTPPEKINNFLQTKVDYHYTVTEVPVWAKTKEIEDAFPD
                     LKKALAGTATGQTTLANAGAGWQVPE"
     gene            complement(6936..7718)
                     /locus_tag="VFA02_08980"
     CDS             complement(6936..7718)
                     /locus_tag="VFA02_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10027.1"
                     /translation="MLNHFFHSRLTLGLAESVITALVALMVMLLARHRAPGILKDLPV
                     AMLRGIIQIVAVGAVLAIMLHGPQWTSLIVLVAMMLAAASIVRKRASRIPRGFRLALV
                     SICAGAGAVLAIMVFFGVIPLTITMLVPVGSMVIANTMNTQSLFLDRLRGEVTSHVGE
                     IESALALGADPDTALLPYLQAACRACLIPSVDNIRSLGIVWIPGIMAGMVLSGEPPLY
                     AAFYQFVVLTTIFSASALTCFVSSHLVAHRIFSAQEQLLLQA"
     gene            complement(7711..8394)
                     /locus_tag="VFA02_08985"
     CDS             complement(7711..8394)
                     /locus_tag="VFA02_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZU10028.1"
                     /translation="MMQMLESRQLGRALPPPESRVLLCDISFALEQGEVLAVVGPSGA
                     GKSTLLRLLNRLDEPTSGEVLLKGVNTKTLPPRELRRRIGMVMQRAYLFPGTVAENLA
                     YGPRQHNHSLSAEEINHLLEQVGLTGYAGRNALTLSGGEAQRVAITRALANQPEVLLL
                     DEPTSALDEAARRGIETLLESLVRQRHLTCVWVTHNMEQARAVADKVLVIEAGKPTAY
                     GPVHEVLRA"
     gene            complement(8517..9221)
                     /locus_tag="VFA02_08990"
     CDS             complement(8517..9221)
                     /locus_tag="VFA02_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU10029.1"
                     /translation="MAETAFLTVEISHRIGGFHLEAGFHLSAPWTVLFGSSGAGKSTV
                     LRAVAGLLTPHKGRVALRNRILLDTSSGISLAPGQRGIGFLTQQPALFPHMSVRRNIA
                     FGLHQLTHDRRESRTKEMLALFRIEKLADRMPSELSGGEYQRVALARSLAPRPELLLL
                     DEPFSGLDAELKTSILKDLSTQTPLLYVSHDLAEAYQTNAEVIVLDNGRIETQGPVHE
                     VLAPKRAQLLRQLGAE"
     gene            complement(9214..9876)
                     /gene="modB"
                     /locus_tag="VFA02_08995"
     CDS             complement(9214..9876)
                     /gene="modB"
                     /locus_tag="VFA02_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZU10030.1"
                     /translation="MDTQALWLTLKLATVTTALLLCIAVPLSWWLAFGKARWKVAVEA
                     VATLPLILPPTVLGFYLLVLMGPRTAFGRFFIHLTGHPLAFSFSGLVIGSMIYSLPFA
                     VQPVATGFSGIRTEILDAARLLGASPLRVLFFVLLPLSRRSLFSAAVLCFAHTMGEFG
                     VVLMIGGDIPGATRTLSIAIYDQVQDFRYTDANRTAFLLLAVTLLALIAVYSMRKQAR
                     SG"
     gene            complement(9908..10939)
                     /locus_tag="VFA02_09000"
     CDS             complement(9908..10939)
                     /locus_tag="VFA02_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZU10031.1"
                     /translation="MRRQRKWHLAGALLMALAFTGSTPSWAQGPYHVQDKWKLGGDGG
                     WDYLAVDPHSGNLYITRGNHVMVVDTASGKLVGDITGLHGTHGVVFDPDGKEGYISDG
                     GGNAVAIFDRTTNKITQTIPAGTNPDGMVYEPSTHTVWAFNGRSRNATVIDTKTKQVV
                     ATMPLPGKPEFPVADGKGSVFVNIEDKNEIAHIDAKTHEIKAEWPICESPSGQAIDVA
                     HNRLFSVCDGKKMSVVDAVSGKVVATPEIGDGPDAAGFDPGTGNAFSSNGDGTLTVVH
                     QNSPDSYTVLQNVATQRSARTMAFDPKTGKIYLVAAEFGPRPAATPENPRPRPSVLPG
                     SFTVIVVSK"
     gene            11092..12663
                     /locus_tag="VFA02_09005"
     CDS             11092..12663
                     /locus_tag="VFA02_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HZU10032.1"
                     /translation="MNRRSRTLAAFLLGISLAGAQSFVFAQEKKPDEPQKGEAQHENA
                     KEQENIPPEKSSVTHHDITLNGRTIHYTATAGTLLIRNDDDKPYGSIFYVAYTEDGAD
                     AKTRPLTFLYNGGPGSASLWLHMGSFGPVRVETASPAATGGAPYQIVPNQYSLLDQTD
                     LVFIYAPLTGYSRAVGKATPKDFTGVDQDLKAFDKFIQRYITVNQRWNSPKVLFGESY
                     GTTRSAGLSAVLQEHGVELNGIVLLSSILNYGELAPGMDTQYIVNLPTYAAIAWYHNK
                     LQNKPSDLKAFLNDVRAFARGEYSEALAQGDQLPAAQFDAVAAKVAQYTGLSVQYVKE
                     AKLRISPFRYRKELLRNEGDILGRYDARFEGTDVDNAGEYPGYDPSDTSISGAFVAAE
                     HDYLSRELKYETTDEYRPSANSIGEWDWHHRGAGAGRSFGGQQAMPYVAGDLADAIRK
                     NPKLKVLSANGYFDLATPFFATEYDLSHMMLTPDLAKNVEFTYYPSGHMVYLNIEALK
                     EFRKDLQGFYSSLTK"
     gene            complement(12715..>12936)
                     /locus_tag="VFA02_09010"
     CDS             complement(12715..>12936)
                     /locus_tag="VFA02_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10033.1"
                     /translation="LEGTARNRGRNLSVPEMPNVDVLMHGDTAIAHGVFSFTGQDGKT
                     MTTRFSDVYVWKDDAWHVLFSQQTPADVD"
BASE COUNT         2868 a         3504 c         3711 g         2853 t
ORIGIN      
        1 gcgactccga aggtcgtacc agtccaaact tcgcctacct gaggatttgt tgtcagaatc
       61 tcgccatttg ctccgattcc attcaccgcg cccatttcac cgtttccaaa gagcatgacg
      121 ttgtaattga agatgcgctt cagggccgag agccgcatct tctttgggac caagtcgcca
      181 agcccggtaa ggttggcgta ccactggcct gcaagctgct cggccataat gtcagagtgg
      241 tactcgctgt tggtgtcgta gtcgaagtac gtgccgttcc agagcttcct gatataagca
      301 gtctgggctt tttggaacaa tgcattgtac tgggcggcgc ttccggggtc gccgaccctc
      361 tgtgcaatct gtgctgtggc gcgcagggcg gccagataca gaccgccgct gtaggcgctc
      421 tcaccgctgg cgatccagtc gtcgtaggtc tggtcaggga agccgccatt ttcaatcagg
      481 ccatcgccgt cggtgtcgta ttgttgcagg tgctccatgg cccgctggac ggagttccac
      541 gaatactgga gaaaggccag gtctttgctt cccgtcagaa catagtcgcg ccaaagaagg
      601 agaacgtact tgctgttcag gtcgcgccag ttggaaacgt cctgatagtt atactggttt
      661 acgtttacga aggggtcttc ctgcggcgcg ccgaggtcat gcggggcagc tcccgctacc
      721 tttctctcca tcgtgacaaa tttgtgctca tgctgtgcct gccatgccca gagatatttt
      781 tgcggattgc tctcctggat cgtgtcagtg tattcgcgca tgatctgttt ttcgatgtcc
      841 ggccagaagc gaatcagcgg gaatgagcca tagaaccgca cgtcgagtgt gccatagaag
      901 acgtaatcga agcattcaag aaagctgaag gtgtcttcgt ccttggttgc tgcgtgagcc
      961 gggttcccga tgccgtccag ttcgtgtgcc catactgttc cgccatcggc gagaatatac
     1021 atctcattga acagctcgcc gcgataccac tcgggaaggg attcgtcttc gatgtacggt
     1081 ttttgccatg cgtcaatggc atggctccaa ttctcatctt ctgcgagcgc ggttttagcg
     1141 atctgccacg cgtgcgtacc ttccgcgcca aagaatttcg tgtaatgacg tacccattta
     1201 cggcctccgc caaactgaat cagcggcagg tcccaggaga gcgccatagg caccatcttt
     1261 ttctcgcccg gtgccagggt aaagcgaact gcaatcgcac cggcaatctg ctcgccgctg
     1321 gatgccacat ccggagcggc attgggcagc cgtccatcat ctgcaaatgg ctcccagacc
     1381 tccattcctg gcccttgtgg atagaaggta gtgagatacg tcacttccat gtcagggctg
     1441 gtctttgtgg cgatggcaaa ctgcccgtcc cattcactgc ggacggctcc gctgcggatg
     1501 cgatcaaaga ggatgccctg aatggtagcg ccgggtacat cttcggaaac gtagcggttc
     1561 ttgtcctgat tgctgagggc cccgctgaag ccgctcgata catcgcggaa ccaacccacc
     1621 atgtttgtcc aggagaagag cagggaaacg gtgactggct tgtctgaggg gttctctgca
     1681 taccagttat agagggcgac cgggtagctg gattccttgt aattgttggg gaggacgggc
     1741 gaaaactgct caacggtcag cttgataccc agttctttgg ggtcgtaagc gaaccacgac
     1801 tttggataca gcgcagcgta ttcacccgcg cctgccggat aagaccagtt ccatgaagaa
     1861 agcgctccgt tgagtggttt gcctgtagac aacgcgatcg cccttccggt ttcaccttcc
     1921 ggttgcacaa aaactgcgaa ctggtttccg ggcacatctt catatttgtg gacgccggcc
     1981 ttgatgtgcc agcgctcgaa atttccgcga taactgcgcg agaaagtgcc ggcgccaaac
     2041 ccgcccacgg gcgcaccctg ccagtagcca tcgtcaataa ggttgggcaa agcgggtttc
     2101 cgtccacctg gattcggggg tggcacgccg ataccaattt tccaggcggc ctgggggata
     2161 tgatcatcgg cctgaagcgg cagacaggca accagggcaa agcatgttaa ccaaataaaa
     2221 cgtttgagca gcatgttttt agttgtttcc gatgccgtca tcatacaagg cttgccggga
     2281 atggggccag tccgggagac gcaagcacgc aaaaatgacg tggataagat gtctcatatc
     2341 cacgcgcaat acgcaagcaa atgagaaaat tgtgttaccg aatgtttccc cgttttgcaa
     2401 taagtgtact ttgtggcttt atgatgaccg gctgcggata ccatactgtc cactccgctg
     2461 cgcaccttcc ggacacggtg cacacgctgg ctgtcccgac gtttgcgaac cacacgcagt
     2521 ccttccacac agaggtctct tttaccaacg ccgtcattcg ggagtttacg agccgcactt
     2581 cgtataaagt gctggcgaca gacaagcccg gtgccggaga tgcggtagta gatggagcga
     2641 tcaatagctt tcaggtggtc ccgctgacct acaacatcca gaccgggcag tcgtcaagct
     2701 atctcatcac aatccatgca agcgtgaagg tgctggaccg caacaagcgc gtgctctacg
     2761 agaacactca ctatactttc cgccagcagt accagaccac gcaggacctg gtgagtttta
     2821 ttcaggaaga cccggctgca gtgcagcgcc tttccaggga ctttgcgcaa gcgctcgtct
     2881 ccgatattct ggagtccttc tgatatgggt gcagggttcg catcaacaga ccgctttctt
     2941 gcggaaatcc gtggcaataa gttgcgggcc gggtacgtgc tcattggcga tgagattttt
     3001 ctttatgaac gctgccgccg tgccctgctg gatgcctttg ttccatctga tctgcgcgac
     3061 ttttgccttt cagaaataga cctcggcgaa accagtatct ttgaggtgct ggaccgggcc
     3121 cagacgcctt cattgatggc gccgtttcag gttctctttg tccggaacct gaaacagctt
     3181 tatacccgcg gtgcaaagaa ggacgagttt gctgccattg atgcctactt ccgttcgcca
     3241 aacccacagg cagtgcttat ctttgttgcc gaccatatcc gcatcccatc tgaccctcgc
     3301 cgcatggaca tgggagacaa ggaccgctat gagcgcatcc gggaaacgtt gggtgaccat
     3361 tgcggaatgg ttgaactggc gcgcgtggac gaggccgacg cgttacgatg gcttctggct
     3421 gaggcggaaa aacagcagat cagggtggag caggacgctg cccgtgagct ggtagacgcg
     3481 ctgggcgcgg acatgatgct cattgccaat gaatttgaaa agctcacgct ctatgtgggt
     3541 ctgaaaaagc acatcacgct gggcgatgtg gagacaatgg tgctggctgc caagcagcgc
     3601 tcattgtatg agctgaccga tgcaatctct gccagggaca aaaagcgcgc gctggcgctg
     3661 ctggaagggc tgctgaatgc gtcagacggc ggcgaagagt cggccattgg acacctttac
     3721 atgctggcac gcaccttccg gcagatgctg gtgattttgg aaaagaacgt ccgcgactcg
     3781 cgcgcgatct ggcaggccct gtggcagggc tttcgcatgc ctccatttgc ggctgaagac
     3841 ctgattcgtc aggcgcggcg gtataaatca aagagggaat tgacgcgtgc tctgcgcctc
     3901 attgcccggg ccgatctgga gcttcgctcc cagcctccag acaagaagct ggtgttggaa
     3961 cgtctggtga tggacctggc cagcgagccc aggaccaccc aggcaggcgc ggcttcgatg
     4021 cagtatgcca tggaaatctg atctggccca gcgcacttgt ttcctataca tggttcgcag
     4081 caacggagag gctttggacc gcttcagagc gccgggtgag ctgtgatgag ttctcccatg
     4141 cttccgatct tttccggaag gcctttgcgc cgcggcaggg cgcttcctag ttacgaatca
     4201 cggagcggat tctcggaaaa ccctgcacgg ccgcgctcat ctcggggtct ttgctgattt
     4261 tttctatcag actgcgcgta atctcgtcgt cagtatcagc ggtgttgcgc agacttaccg
     4321 gatagcgcac aaccgcttcg agccctgtgc cggtgagttg cagttgtgct gttggtttta
     4381 agggtgtcag gggaagctcg aaacgcattt ccagattgcc atgctggcga tcgagcgcgt
     4441 cccggtattt tttgtaggca ttgttgacga cctccagcat gttcgactcg accaattcgt
     4501 aattggccga gggttgcagt gacacggcga cttcatgcca tgtgtaagcc gttcctggaa
     4561 actggcggaa catcggtgtt gttgcctgaa aaaggacgga attagcaaat gcgacaatgc
     4621 gtccggtagg atgcaggtcc gctcctgttc ctgcgagttc gagcagatag aagcgcacga
     4681 accccacgtc aaccacatcg cctgttacgc ctgcaatgct gatgcggtcg ccaacgcgaa
     4741 tgccgtagcg gccgataagg aagaaatacg ccgcgacaca gagcaggacg gtctgcaatc
     4801 cgacagcgag gcccgcagtg atgaatccag cgaatgtcgc cagcgaactg aattcgctga
     4861 tgacgctgaa gacaagcacc aggccaatca ggaatccaat gacaaagcgg cgaacgacaa
     4921 ggaattgcct ccttctgcgg acgtccgtga tatagcggaa gatggcgcgc ttccaaagat
     4981 tcgagaccag aagaagaatg ccgagcacgg cgatgagaat ggccacggta atgaagatgg
     5041 agcgcagaat cgagccgtac tggctggcga ttgaggcccg ccagtccaga agattggctt
     5101 tgctttcttc aagcgctgag gcttcctggc ccagcggaat ttctaccgca gcaagctggt
     5161 tgaattttgt agcgaggtca tcaaagtcct tcttgccggg cggtgtttga ccgggctgag
     5221 gattgtcggc tgcagccgaa agcgcgcgtc cttgttgcac ggtctctttc atcgcgctac
     5281 gcaaaggggt acgcatggca tttaccaggt cttcgagctt tgcggtctgg tctgtcagcg
     5341 tatcaatctc gtgcagcgtc atggactggc tgtaaaggcg gcgcagttga ccaatcagtc
     5401 cgttggcaga agcaatttgt ggcgtgctgg atggagttga aggtttaccg ttgaagactt
     5461 cgggtacaga gctcttcagt tgcgccacgc tggccgcaaa tccatttgga gaaacctggt
     5521 tcttgctctg tgcctgctgc ttgctcttct ggttctgctt tgtgtttgtg gagacctggt
     5581 ttgtggtctg cgtaagctgc atgagcgagt tcagcatcgc ctgtcccagg tccaactcgc
     5641 cctgcaagcg atcgcgctgg tccagcaatg gttgtgcatt gcggccggga gcggctgcga
     5701 tcctatcatt taactgcgcg atctgctttt ccagatcagc attgtgtgct ttggtatcat
     5761 cgaggaactt cagcaactgc tgccgcgagg tgggagaagc ggcctcgtta ttttcgttcg
     5821 ggaccagagg cgctgccttc tctgcagact caaacgcata cctgacggcc ttcgctgcga
     5881 tggattcagc attggcctga tagagtgcgt cggtaggcag tccggcaggt tccacctgtg
     5941 tctttacgtt tcgataccag gtaatcaccg cattcaggtg actcagaatc tggtcgtccg
     6001 ttacaagtgg cgcattcccc tgctgggctg aaaaaggtaa cgctgcgaaa acaagaagga
     6061 caatcagaaa agagcgtgca aacgtggggg acttgaattg cattgtcttt tccattttat
     6121 ctatgctcgc cgttatggct gaactccagt gcgacaaatt gtcgccagaa aactgctctg
     6181 atccagactg ctttttccag cttttctgct tcttagtcaa agacaaagga gccaccctat
     6241 gaaagaccca ggatggacga gaacagtttc tgttgcagca ggcattctgc tcaccctcag
     6301 tgcgtgttcc tccggcagga acaagccaac gcctgagaac tttaaggccg cgctgaatag
     6361 ttatttcaca aaccgcagcg aatgcctttt tccctcagcg ccgcagtttc catacgaggt
     6421 aagcccttct cctacggcaa aggctgaaca gaagcgcatg gatgcgttaa tggcggcagc
     6481 actggtgaag cgcaaggccg acgagagtat ccacgtctat atctactcgc tcactcctgc
     6541 aggagagcgg gcaaccagcc gcttctgcta tggacacaga caggtcacca gcattgataa
     6601 ctttacgcct ccggaaaaga tcaacaattt tcttcaaact aaggtggact atcactacac
     6661 cgtaacggag gtcccggtct gggcaaagac aaaggagata gaagacgcct ttcctgatct
     6721 gaaaaaagct ctggcaggaa ccgctacagg gcaaacgaca ctcgcaaatg ccggggccgg
     6781 atggcaggtc ccggaataaa ttcctgtata cgccttgtct atgctgactg cctttccagt
     6841 gtgtttctta atgaatcgaa gctatgaaac gggcaaaggc agttgcggtg gccgcctctg
     6901 ttctggaggc cttaagagtg cctgaatatc tttgattatg cctgcaacaa aagttgctcc
     6961 tgcgccgaga agatgcgatg cgccaccaga tggcttgaca caaaacaggt aagtgccgat
     7021 gcggaaaaaa ttgttgtcag cactacgaat tgatagaagg cagcatacaa cggaggctcg
     7081 cccgaaagta ccattcctgc cataattccc gggatccaga cgattccgag agagcgaata
     7141 ttgtctacag aaggaatcag acaggcgcga caggcggcct gaaggtaggg aagcagagcg
     7201 gtgtctggat ctgctcccag cgcaagggcc gattcgatct ctcccacatg ggatgtaact
     7261 tctccgcgga ggcggtcgag gaaaagcgac tgcgtgttca tggtgttggc aatgaccatg
     7321 cttccaaccg gcaccagcat ggtgatggtc agcggaatca ctccaaagaa gaccatgatg
     7381 gcgaggactg cgcctgctcc tgcgcaaatg gagacaaggg ccaaacgaaa gccacgcggg
     7441 atgcggctgg cgcgcttgcg cacgatactg gcggcggcga gcatcatggc gacgagaacg
     7501 atgagcgatg tccactgcgg cccatgtagc atgatggcca gcaccgcgcc cacggcgaca
     7561 atctgaatga ttccgcgcag catggccacg ggaaggtcct tcaggattcc gggtgcgcga
     7621 tgccgcgcga gcaacatgac catcagcgcg accagtgcag tgatgacgga ttctgcaaga
     7681 ccgagtgtga ggcgggaatg gaagaaatga ttaagcacgg agcacctcgt ggacgggccc
     7741 atatgctgtt ggctttccag cttcgatcac gagcaccttg tctgctaccg cacgggcctg
     7801 ttccatgttg tgcgtcaccc agacgcaggt aagatgccgc tgccgcacaa gcgattccag
     7861 cagggtctca atgcctcgac gggctgcttc atccagtgcc gaggttggtt catccagcag
     7921 aagcacttcg ggctgatttg ccagtgcgcg ggtgatggca acgcgttggg cttcgcctcc
     7981 ggaaagtgtg agggcgttgc gtccggcgta tccggtaagc cccacctgct ccagcagatg
     8041 attgatttct tccgcggaca ggctatggtt gtgctgcctc gggccgtatg caaggttttc
     8101 ggcaactgtt ccggggaaga gataggcccg ttgcatcacc atcccgatac ggcggcgcag
     8161 ctcacgcggt ggaagtgtct ttgtattcac tcctttgagc aggacttcgc cgctggttgg
     8221 ttcatcgagg cggttcagga ggcgcagaag cgtactctta cctgctccgc tggggccgac
     8281 gaccgccagc acctctccct gctccagcgc gaagctgata tcgcagagta atacgcgcga
     8341 ttcaggcggg ggaagtgcgc ggccgagttg tctcgattca agcatttgca tcatgaaacc
     8401 aaaaatcacc gctcagggaa ctgtcacctc gactggagcg aaaagtggag gacctggata
     8461 tgtttcggac gaatccgctt accaccacat cgttctgctc cgccttggat gacggttcat
     8521 tctgctccga gttggcgcag gagctgtgcg cgtttcgggg ccagtacttc atgcacaggc
     8581 ccttgtgttt caatgcggcc attgtcgagc acaatgacct ctgcatttgt ctgatacgcc
     8641 tctgccaggt cgtgggaaac ataaagcagc ggtgtctgcg tcgagaggtc tttaagaata
     8701 gaggtcttca actccgcatc gagtccggaa aacggctcgt cgagcagaag cagttcaggg
     8761 cgcggcgcga gggaacgcgc cagagcgaca cgttgatatt cgccaccgga aagttccgag
     8821 ggcatgcggt ctgccagctt ctcgatgcgg aagagcgcca gcatctcctt cgtgcgcgat
     8881 tcgcgtcggt cgtgcgtaag ctggtgcagc ccaaaggcga tgtttcgccg cacgctcatg
     8941 tgtggaaaca gcgctggctg ctgtgtaaga aagccgatcc cgcgctgtcc cggagcaagg
     9001 gagataccgc tggatgtgtc aagcaggatg cggtttcgca atgcaactcg tcctttgtgt
     9061 ggtgtaagaa gtccggcgac agcgcgaagc acggtgctct tgcccgcgcc ggatgagcca
     9121 aagagcaccg tccagggcgc agaaaggtga aagcccgctt caagatgaaa gcccccgatg
     9181 cggtgcgaaa tctcaacagt gagaaatgca gtctcagcca ctgcgtgcct gctttctcat
     9241 gctgtagaca gcaatcagcg ccagcagagt cacggccagc agtagaaatg cggtgcggtt
     9301 cgcgtcagta taacggaagt cctgtacctg gtcgtaaatg gcaatggaga gtgtgcgcgt
     9361 tgcgccggga atgtcgcctc caatcatgag cacaacgccg aattcgccca tggtatgcgc
     9421 gaaacaaaga acagcagccg agaacagaga ccgccgggaa agtggcaaaa gtacaaaaaa
     9481 gagcacgcgc agcggtgatg caccaagcag acgggctgca tccaggattt ctgttctgat
     9541 accggaaaag cctgtcgcga ctggctgcac ggcaaagggc aggctgtaga tcatggagcc
     9601 aatcacaagt ccgctgaagg agaaggccag cggatgcccc gtaaggtgaa tgaagaagcg
     9661 tccaaaagcc gtacgtggcc ccatgagcac gagcagataa aagccaagta ccgtaggagg
     9721 caaaatcagt ggcagggtgg caacggcttc taccgcgact ttccatcgcg ctttgccgaa
     9781 ggccagccac catgacaacg gaacagctat gcacagcagc agtgctgttg tcacggtggc
     9841 gagtttcagg gtcagccaga gcgcctgcgt gtccataccg gagtgcgata ggacatttta
     9901 tcttgcttca cttgctgacc acgatgacgg tgaaactacc cggaagcacg gacgggcggg
     9961 gccgcggatt ttccggagtg gctgcgggcc gcgggccgaa ttcagccgcg accagataga
    10021 tttttccggt cttcggatcg aacgccatcg tgcgcgcgct gcgctgcgta gcaacgttct
    10081 ggaggaccgt atacgaatcc ggagaattct ggtggactac ggtcagggta ccgtctccat
    10141 tggaactgaa agcattgcct gtgccggggt cgaatcccgc agcatcggga ccatcgccaa
    10201 tctccggtgt agcaaccacc ttcccgctaa cagcgtctac gacggacatt ttcttgccat
    10261 cacagaccga gaaaaggcgg ttgtgcgcaa catctatcgc ctggcccgag ggcgattcgc
    10321 agatgggcca ttcggctttg atttcgtgcg tttttgcgtc aatgtgcgcg atttcgttct
    10381 tgtcctcgat atttacaaag acggagcctt tgccgtcagc aacagggaac tcaggtttac
    10441 cgggaagcgg cattgtcgcg accacctgct ttgtttttgt atcaatcacg gttgcattcc
    10501 tgctgcgtcc gttgaaggcc cagacagtat gggtagatgg ctcgtacacc atgccatcgg
    10561 gattggtccc cgcagggatg gtctgcgtaa tcttgtttgt cgtccggtca aaaatggcca
    10621 ctgcattgcc tccgccatca ctgatgtatc cctctttgcc atcagggtcg aagacgactc
    10681 catgcgtgcc gtgaaggccg gtgatgtccc caaccagctt gccggaggcg gtgtccacga
    10741 ccatcacatg attgccgcgg gtgatgtaaa ggttcccgga atgagggtcc acggccaggt
    10801 agtcccatcc gccatcaccg ccgagtttcc atttgtcctg cacatgatag gggccttgcg
    10861 cccacgatgg cgtgctgcct gtaaatgcaa gtgccatcaa cagggccccc gccagatgcc
    10921 attttctttg ccgccgcata aaattttcct ttctggagca aaacagattc cgcacctgac
    10981 tgtattcggg ttttccttaa aatgtcctga atgaactatg aagttatata gtgaaacaaa
    11041 tccagaatcg tattcgtcct gaagattact ttctatagga ggacaagcac attgaacaga
    11101 agatcgagga cacttgctgc ctttttactt ggaatttcgc tggctggcgc gcaatccttt
    11161 gtatttgcgc aggaaaagaa gcccgacgag ccacagaagg gtgaagcgca acatgaaaat
    11221 gcaaaagagc aggagaatat tcctcctgaa aaaagctcag tcacgcacca cgatattacg
    11281 ctgaacggca gaaccattca ctacaccgca accgccggga cattactgat ccgcaatgat
    11341 gatgataagc cttacggaag catcttttat gtggcatata cagaggacgg cgcggacgcg
    11401 aagacgcggc ctctgacgtt tctttacaac ggcggccccg gatcggcttc gctctggctg
    11461 cacatgggtt cattcggccc ggtgcgcgtg gaaacggcca gccccgcggc cacgggcggc
    11521 gcaccttatc agattgttcc caaccagtac agtctgctgg accaaaccga cctggtcttt
    11581 atttacgcgc cgctgacggg gtactcgcgc gccgtgggca aggccacacc caaagacttc
    11641 accggggtag accaggacct gaaggctttc gacaaattca tccagcggta tatcacggtg
    11701 aaccaaagat ggaattcacc taaggtcctg tttggagaat cgtatggcac gacccgttcc
    11761 gccgggcttt ccgctgtttt gcaggagcat ggcgtagaac tgaacggcat tgtgctgctc
    11821 tcgtccattc tgaattatgg tgaacttgcc ccgggaatgg atacgcagta catcgtcaat
    11881 ctgcctacat atgcagccat tgcctggtat cacaataagc tgcaaaacaa gccgtcggac
    11941 ctgaaagcgt ttttgaacga tgttcgcgcc tttgcgcgtg gggaatactc tgaggccctg
    12001 gcacagggcg atcagttgcc cgcagctcaa tttgatgctg tggctgcgaa ggtggcgcag
    12061 tatacgggcc tgagtgtgca gtatgtaaag gaagccaaac tgcgcatctc gccattccgc
    12121 taccggaaag aactgctgcg caatgaaggc gacattctgg gccgttacga cgctcgcttt
    12181 gaaggcactg acgtggacaa cgcaggcgaa tatccgggct acgacccgtc tgataccagc
    12241 atttccgggg cgttcgtcgc cgctgagcac gattatcttt cgcgcgaact gaaatacgag
    12301 accaccgatg agtaccgccc cagcgccaac agcatcggcg agtgggactg gcaccatcgc
    12361 ggagcaggcg cgggccgcag ctttggcgga cagcaggcca tgccttatgt tgccggagac
    12421 ctggcggatg ccattcgcaa aaatcctaag ctgaaggtgc tctccgcgaa tggatacttc
    12481 gacctggcta ctccattctt tgccacggaa tatgatcttt cccacatgat gctgacacct
    12541 gatctggcga agaacgtgga gttcacctac tatccgtcag gacacatggt ctacctgaat
    12601 attgaagcct tgaaggagtt caggaaagac ctgcaggggt tctactccag tttgacgaag
    12661 taggctgcta cgcgtcccac gacccagcat ggccgcgggg cgcatgcagg aaattcagtc
    12721 cacatcggca ggggtctgtt gtgaaaagag cacgtgccat gcgtcatctt tccaaacgta
    12781 aacatcggaa aagcgcgtag tcattgtctt tccgtcttgc cctgtaaatg agaacacacc
    12841 atgcgcaatg gcggtatcgc cgtgcatgag tacgtctacg tttggcatct cgggaacgga
    12901 gaggttccgg ccgcggtttc gcgccgtgcc ttcaag
//
LOCUS       DASRBV010000241         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_269106, whole genome shotgun sequence.
ACCESSION   DASRBV010000241 DASRBV010000000
VERSION     DASRBV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269106"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFA02_09015"
     rRNA            complement(<1..951)
                     /locus_tag="VFA02_09015"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1788..2483
                     /locus_tag="VFA02_09020"
     CDS             1788..2483
                     /locus_tag="VFA02_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZU10034.1"
                     /translation="MNEYIPSRFSRRGFLTGITASLLSSASLDILTPPSLSAQTTLTP
                     EEALKALLDGNERFVAGRMTSFDEDLKILKARTIDKQEPFAAVLSCADSRVPVEIIFD
                     QTIGHIFVTRVAGNMATSEVIASLEYGAAVLGTKVILVLGHQNCGAVKATMQGKQVPG
                     QISALYPYIQPAADEAGGDLEKAIQTNARIQAHLLRESSPVLASLVKENKLKVVAGQY
                     NLASGKVSLLEGA"
     gene            complement(2511..3428)
                     /locus_tag="VFA02_09025"
     CDS             complement(2511..3428)
                     /locus_tag="VFA02_09025"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZU10035.1"
                     /translation="MKVRFAGIELRNPVIAASGTFAYGIEFEDIVSLDRIGGFVTKGL
                     SREPMAGNQAPRIIETPAGMINAIGLQNIGVKAFIENKLPALGKYPSCAVIANVFGHT
                     VEDYVAVIEALNEARGIAAYELNASCPNTKHGGMSFGSDWKLLGELVVAAKQAATRPL
                     IVKLSPNVTSIAAMARCAEDAGADAISLVNTFVSLSIDVSTRRPRIANVTGGLSGPAI
                     KPIAVRMVWEASKAVQIPVLGMGGIAAPEDAVEFMLAGATAVQIGTASYADPRAVERI
                     AHGLEHWCVRHHIERVSSLTGGLELGPAQ"
     gene            complement(3440..>4549)
                     /locus_tag="VFA02_09030"
     CDS             complement(3440..>4549)
                     /locus_tag="VFA02_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU10036.1"
                     /translation="AFNSSGRLQQAEAEANVPAPKNFETEVGRTPAAISYELDFAKAL
                     IQGEQLGKHNVTDMLTLSISSTDILGHGVGPDADEQHALVDALDADLDDFFTWLDKNI
                     DGGLGNVWIAVTADHGVAPSPAVAAQLGMNAAQIDTGRLITLLNDAMNQKFSPGEKVE
                     YILPHQELPYISLNQPSFERAGINEQEAETAVQDALPAVFQSLTKLLDTPLPAQSKLP
                     PRPVLYRSYTRIQLANGDLPHTQFGDLLAHSYTPNGGWYVRVVPAAFQMGLEKGNGTT
                     HFTPYSYDRHVPLGFYGAPFAPGIYHGRVEPVDLAATFASLLGVNQPSASVGHILTQA
                     FKPAAAVIYPKPAPTRPHRSARAATKKATPEEQHQ"
BASE COUNT          976 a         1402 c         1133 g         1038 t
ORIGIN      
        1 cgagcccagg taaggttctt cgcgttgcgt cgaattgaac cacatgctcc accgcttgtg
       61 cgggcccccg tcaattcctt tgagtttcag ccttgcgacc gtactcccca ggcgggatgc
      121 ttaacgcgtt agctgcggca cagcgggatt gggtacccgc tacaccaagc atccatagtt
      181 tagggctagg actaccaggg tatctaatcc tgtttgctcc cctagctttc gcgcctcagc
      241 gtcagttgtg gtccagtgcg ccgctttcgc cacaggtgtt cctcccgata tctacgcatt
      301 tcaccgctac accgggaatt ccacgcacct ctcccacact ccagcacggc agtttcgcct
      361 gcattttccc agttgagccg ggagatttca caggtgactt accgcaccgc ctacgcgccc
      421 tttacgccca ataattccga acaacgctcg caccctctgt attaccgcgg ctgctggcac
      481 agagttagcc ggtgcttctt ccacccgtac cgtcagtatc gtccgggtcg aaaggagttt
      541 acgtcccaaa ggacttcatc caccacgcgg cgttgctgcg tcagggtttc ccccattgcg
      601 caaaattccc cactgctgcc tcccgtagga gtctggaccg tgtttcagtt ccagtgtgtc
      661 cgtgcgccct ctcaggccgg atacagatca tcgccttggt gggccattac cccgccaact
      721 agctaatctg ccgcgacccc ctcctcaagc gaattgctcc tttgacccgt gggtattatg
      781 cggtattagc ctcggtttcc cgaagttatc ccccactcga gggtaggtca gtcacgtgtt
      841 actcacccgt gcgccacttt actcagggcc gaagcccctt tctcgttcga cttgcatgtg
      901 ttaggcacgc cgccagcgtt gattctgagc caggatcaaa ctctcgtttg aaacctgatg
      961 attgcacttc gccgacggag gtcagcgagg caatcgcccc gcaatgctcg aaagattgcg
     1021 aggactaaca ttgtgagaat gatcaaaccg atcttcttcc attctcacga ctggcatgtt
     1081 caacctgatt gtcaaagatc ctttcgcaga accgcctgag cggactgctt ctggatcgag
     1141 tgcgcgacca ttgcgcgcgt cctcgaactt gttgcgcctc gtttttgcgc gaacttttta
     1201 agaatctcaa accttagcga gaatgtcaag ccattttcat aaattcttga tttttctcgt
     1261 ttccatcttc caccaggcat gaaatttacc cgccgaaagt ccggattcca aaagccgcta
     1321 aacggcgcaa aacctgaaaa agatcgaccg cagagcgcac tcggcggcgg gcacttggcc
     1381 cgaagggagc taaactccct gcctgcaatg gattgtccgt tcagattagc gtcctaaacg
     1441 ccctttgaac tatgtccagc ttcagtcatc cgctcattgc cattcaaggc cgggggcgaa
     1501 gctgctcatt cccactgctt cttgtcgctt tcttaatata gcctgactgc ttcacgcttg
     1561 caagccgtca gcctttcatc aacagcaccc ttctaggcgc cttgccatca gccagcgaca
     1621 agaccaatcc taccaacgga cgacccgctc cgcaactgtt gaaaactgtg aatgtctgtt
     1681 gaaaaacact ttctatccag tgtttatgcc tgttttcaaa ttggcatata tactctccac
     1741 aaattgctct ttcaggctgt caatttgcac gtggaggcat aacgtacatg aacgaataca
     1801 taccttctcg tttttcccgc cgtggatttc tgacaggcat aacggcaagc cttctctcat
     1861 ctgcgtcgct ggacatactc accccgccat ccttgtccgc ccaaaccaca cttacgccgg
     1921 aagaggccct caaggccctg cttgatggaa acgaacgttt tgttgccgga cgcatgacca
     1981 gcttcgacga agacctgaaa attctcaagg cccgcacgat agacaaacag gagccttttg
     2041 ccgccgtcct ttcctgcgcc gattcccgcg ttcccgtcga gatcatcttc gaccagacca
     2101 ttggccacat tttcgtgacc cgtgttgctg ggaatatggc cacctcagaa gtcattgcca
     2161 gcctggaata cggcgcggca gtactgggaa caaaagtcat tcttgtgctc ggtcatcaga
     2221 actgcggagc tgtcaaagca accatgcagg gcaagcaggt tcccgggcag atcagcgccc
     2281 tttacccata tattcagccg gcagcagatg aagccggagg cgacctggag aaagccatcc
     2341 agacgaatgc gcgcattcag gcacacctgc tgcgcgagtc ctctcccgtc ctggcatctc
     2401 tggtgaagga aaacaaactc aaagtagttg ccggacaata caatcttgct tccggcaaag
     2461 tttcgctgct tgagggcgca taaagcagcc ggcagacatt gcgggcctgg ttattgagca
     2521 ggccccaact ccaacccgcc cgtcaggctc gacacgcgct cgatatggtg cctcacgcac
     2581 cagtgttcca gcccatgcgc aatgcgttcc acggcgcgtg gatcggcata gctcgctgtc
     2641 cctatctgaa cagccgttgc cccggcaagc ataaattcca ctgcgtcttc cggagcagca
     2701 atgccgccca ttcccagcac aggaatctgc acagcctttg atgcctccca gaccatacgc
     2761 acagcaattg gcttgatcgc aggaccggaa agccctcccg taacattggc aatacgcgga
     2821 cgacgcgtgc ttacatcaat cgaaagcgac acaaatgtgt tcaccaggga tatagcgtct
     2881 gcgccggcat cttcggcaca acgcgccatc gcagctatcg aggtcacatt cggcgagagc
     2941 ttgacaatca gcggacgcgt cgctgcctgc tttgcggcta ctacaagttc acccagcagc
     3001 ttccagtccg agccgaatga catgccccca tgcttcgtat tcgggcacga agcattcagc
     3061 tcataagcag caatgccgcg cgcttcattc agcgcctcaa tcactgcgac ataatcctcg
     3121 accgtatggc caaaaacatt ggcaatgacc gcacagcttg gatacttccc taatgcaggc
     3181 agcttgtttt caataaatgc cttcactccg atgttctgga gcccgatggc attgatcatt
     3241 ccggcaggag tttcgatgat gcgcggcgcc tggtttcctg ccatcggctc gcgcgaaagg
     3301 cccttcgtta caaacccgcc aatgcggtca agggaaacaa tatcttcaaa ctcaatgccg
     3361 tacgcgaacg taccgctggc ggcaatcacc gggttgcgca gttcaattcc ggcaaaccgg
     3421 accttcatat ctggcttcac tactggtgct gctcctctgg tgttgctttc tttgtggctg
     3481 cgcgcgcgct gcgatgcggc cgtgttggtg caggtttagg atagatgacc gcagcagcag
     3541 gtttgaaggc ctgcgtcaga atatgcccta ccgatgccga cggctggttc accccaagca
     3601 gcgaagcgaa ggtcgccgcc agatcgacag gctcaacacg cccgtggtag atacccggag
     3661 cgaatggcgc tccgtagaat ccaagcggca catgcctgtc atatgagtac ggtgtaaagt
     3721 gcgtagtgcc atttcctttt tccaggccca tctggaaggc ggccgggaca acgcgcacat
     3781 accaccctcc attcggcgta taactgtgcg ccagcaggtc gccgaactgc gtgtgcggca
     3841 ggtctccatt ggccaactgg atgcgggtat agctgcggta gagtactggc cgtggcggca
     3901 gctttgattg tgccggcaga ggcgtatcca gcagtttcgt caaagactgg aagaccgcag
     3961 gaagcgcatc ctgcaccgct gtttctgcct cctgctcatt gattccggca cgctcaaacg
     4021 aaggctggtt cagcgaaata taaggcagct cctgatgcgg aagaatgtac tcgaccttct
     4081 cgccgggaga gaacttctgg ttcatcgcat cgttcagcag agtgatgagc ctgcctgtat
     4141 caatttgcgc ggcattcata cccagttgcg ctgccacagc aggagaaggc gcaacaccgt
     4201 ggtccgcggt tacggcaatc cagacattgc ccagaccgcc atcaatgttc ttgtccagcc
     4261 acgtaaaaaa atcatccaga tcagcgtcga gcgcgtccac tagcgcgtgc tgctcgtccg
     4321 catccggtcc tactccgtgg ccaaggatgt cggtactgga gatgctcagt gtcagcatgt
     4381 ccgtaacgtt atgcttgcct aactgctcac cctggatcag cgccttggca aaatcgagct
     4441 cgtagctgat ggccgccggg gtccttccca cctctgtctc aaaattcttc ggggcaggaa
     4501 cattggcttc cgcttcggcc tgctggagcc gcccgctgga attgaaggc
//
LOCUS       DASRBV010000242        11382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_420558, whole genome shotgun sequence.
ACCESSION   DASRBV010000242 DASRBV010000000
VERSION     DASRBV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420558"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFA02_09035"
     CDS             <1..733
                     /locus_tag="VFA02_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZU10037.1"
                     /translation="VIFDYGMVLSHPQDPAALNNILAITGLDRDTFDRHYWTHRHAYD
                     MGKLNGRTYWQQFAQDTGIRLTPTDVDRLVENDVLMWCTINEPMLAWAKSLAEAGLRI
                     AILSNMGEETLSYMRQEFAWLGDFHHHTWSCELGIAKPDPAIYTYTCEKLGVTPRETL
                     FLDDKLENVEAARRVGLNALQFRNIEQLRHDLASHGWLDGLPVPEKSPKTSHKRNSTL
                     ACCNRAKALLCFPQKGRAAYFFLGD"
     gene            781..1131
                     /locus_tag="VFA02_09040"
     CDS             781..1131
                     /locus_tag="VFA02_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU10038.1"
                     /translation="MSPFKKSEPRDLFPGALEMMVLQSVRRQPMHGYALVQHIKQRSN
                     DLLQVEEGSLYPALQRLLKEGLLKAEWQISPTNRRVRTYSITRAGIRHLEHEVSRFEQ
                     MLEGIHLVLSPTEP"
     gene            1144..3795
                     /locus_tag="VFA02_09045"
     CDS             1144..3795
                     /locus_tag="VFA02_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10039.1"
                     /translation="MFSRRRIYDDLSEEIRQHLEQKIESLMADGLSREEAEHQARREF
                     GNVTLIEERGREPWQWPGLYSLWADVRFGLRQIRRSPSFTVMAVLTLALGIGANTAIF
                     TLIDSIMLRPLPYPHQDRLTVLSSGGAYFPKGWVRALAEHSQSFSSISGYGPNAESNI
                     DVGDSAERVFGSRVSVNVFDTLGIHPEAGSFFAPENAIAGQDQVVVLSDGYFKQRFGG
                     SPLVIGQTIRIDGISRRIVGVMPAGIHFPYAETQFVIPVSFKGGDPYDPWVGPYDLQA
                     IGRLKDGATPSSAQAELRRLHPQLLNLFPWRMPDDWAAEINVVPLLDFIVGDTRPRLL
                     LLFGAVGLILLIACANVANLMLARAASREREMAIRGALGASGSRIIRQLLIESGLLGI
                     LAGIVGLVASGISLRTLTRLLPSDTPRIANIGLHWNVYWFALLISVLAGVLFGLVPAL
                     KMASPNLQQSLRSGSLSVVGKGPQFGLSMLLVIGQIGLSVVVITAAGLMLRSLYSLSR
                     VNPGFRVSQIVTAEVSLDADTCPYLASQADGERGRCQAFFRELPDRARAIPGVEDAAL
                     ADTLPMSGNDQIFAYDAEGHPREARQQPLLAISRVVSTDYFQTLGLKLLRGRLLTGSD
                     QSGSSRAVVINQKMAEDLWPNQDPLGKHVLNLTDEKSPGVFDPNVASIVVGVVSNTRH
                     QKLAGDHFQEEIYFPMTPKLEQPSMHILLRGHASVEQIASGLRRVIAQLNPQVPVTHV
                     RTFDQVIAASDAAPRSLTILLLSFGLLALGVGGAGVYSLIAYIVSWRTREIGVRLALG
                     ASRRQIVLMVLRQSLIWAVAGSLTGLVAAVAFAQMLRRFLFEVSPFDPLTISLVPLLM
                     LLIALLATWLPARRAASIDPMQALRMD"
     gene            complement(3831..4298)
                     /locus_tag="VFA02_09050"
     CDS             complement(3831..4298)
                     /locus_tag="VFA02_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZU10040.1"
                     /translation="MLFVRACSTILLTAALIATPAFAGRSHRAATSGHSKKKAVKHQV
                     IGQRGIDSERATEIQQALIREKYLSGQPSGQWDEETEAAMQKYQADHGWQTKLVPDSR
                     ALIKLGLGPDHSGALDASSTAPANTSPAPAPSAPVTPSVAAANTLASAHTIRD"
     gene            complement(4442..6076)
                     /locus_tag="VFA02_09055"
     CDS             complement(4442..6076)
                     /locus_tag="VFA02_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZU10041.1"
                     /translation="MKSLLERLRLRRLTTTFTVLATLSAGIIIGSVVSHGVHGQEAKV
                     DSSDATPLKVPAPRDLSTDFTKIAKEVGPAVVNINTETLPKEQQRGLRRFHRNPQNPD
                     NNDNNDNNDNNDDNDDQGGMQDFFHRFFGMGPDFGQPDDQAHEALGSGFIVDPKGYII
                     TNNHVVEKADRIYVKLASDPEGDQGHPAKVIGTDKATDLAVIKIDVDRPLPTVKLGNS
                     DSAQVGDWVIAIGSPFTLQQTVTAGIISAKNRTIQGGPQGQFQHFLQTDAAINPGNSG
                     GPLVNMAGEVVGVNTAIFTQSAGYQGIGFAMPSNTVIGVYNALIGPEHKVVRGSIGIR
                     FQQNVSSAVQRVYGIKNGVIISAVTPGLPADKAGLKPNDIIVSVDGHPVKDGDDLVSQ
                     ITARKPGDSVQIGYLRNGQQHTATVSIIDFSKMEAASGEAEEQGPGGGGEGGVDTSQS
                     KLGITVSDLPQNAPPNLHGVLIQRVKPGSFADNVLNMGGMEGQVITSINRQEVHNKAE
                     FNAIVSGLKSGQDVVLSVVDPQRPSAGSFLVGGTLP"
     gene            complement(6259..7191)
                     /locus_tag="VFA02_09060"
     CDS             complement(6259..7191)
                     /locus_tag="VFA02_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU10042.1"
                     /translation="MSRSLRAHLLLIVVVFVWGATFVLIKDALQDISPLLFNLLRMAL
                     AFACLATVYRRHLRDLTRHALSYGALVGLCLAMGYQFQTAGLRLTTPSKSAFLTGLMV
                     VLVPLLSAIPFLRTANSRVPGWNACLGALLAFLGIVLLTTPAHVTFGFSSMNAGDVLT
                     LGCALGFALHVLALAHALPHVPFTQLAVLQVGFCALFMAISGPMLEHFWIHWTGRVMV
                     ALGVAAILATAAAFTVQSYAQQYLPPTHTALILALEPVFAWLTSFVVLGERLSWRASM
                     GALLILGGITFAELLPSVMPATAHEMAAVREEKS"
     gene            complement(7219..8751)
                     /locus_tag="VFA02_09065"
     CDS             complement(7219..8751)
                     /locus_tag="VFA02_09065"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZU10043.1"
                     /translation="MAAVSVGSFADKSSTVKTDKGKVRGYVSADGKVRIFKGIPYAAP
                     PVGPLRWRPPQPAAKWHGTLDATNFGHHCVQASVFADMLFRDPGASEDCLNLNVWTPA
                     KDKNAKLPVMVWIYGGGFYAGAASEARQDGENLAHKGVVVVSMNYRLNIFGFLVHPEL
                     TAESPHHASGNYGLMDQAAAIQWVRRNIKNFGGDPNNITIFGESAGSFSVSAQMASPL
                     SKDLIHRAIGESGAAFSQREPFLPREQREKEDVEKLQKAFGTSKLSDLRAMSADDLLK
                     AVVSRPGHPAARFWPDIDGYFLPDDVASIYAAGKQAHVPLLAGWNRDEGNIPQKAMTI
                     AEYRQFAQKEFGAHANQFLSAYAAASGTEALRALRDYAGDRFLVYATWRWIEAQQATG
                     GQPVYRYHFELPSPGDKFHAPGTAFHSDDIEYVFGTLDSRKGMMVRPEDKQLSELMQS
                     YWTNFAKTGDPNGPGLPKWPVYSSATGWQVMHLNAESTARPDKTRVRYEFLNEVWSKP
                     TQ"
     gene            8858..9928
                     /locus_tag="VFA02_09070"
     CDS             8858..9928
                     /locus_tag="VFA02_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU10044.1"
                     /translation="MSTSTATFEDIRLKVHAGERISSEEALWLWRNASDGELRGLASA
                     VRARFHAPKSCTYMVMRIINYTNVCVAQCDYCAFYRLPGDKQGYVLSQEQVFAKLDEL
                     LALGGDLAAFNGGFNPHLPLDYYCGLFAAIRARYGKRLEFYALTIAEFMYLADHARLD
                     YATAAQRFKDAGVRWITGGGSEILTEDFRSRHSKFKYTVAEYFEAQRAIIEAGLNTTA
                     TMVIGFDETIDERLEHLERTRRFQDETNGLASFLCWTYKPYFTQLGGIEVTTAEYLRH
                     LALCRTYLDNVPRIRTSVLTQNERALEGLLYGADDFDLPIEDEVTQKAGATISLEFDK
                     ILDVARRLGFDPSYRSVAATTL"
     gene            complement(9945..11294)
                     /gene="glmM"
                     /locus_tag="VFA02_09075"
     CDS             complement(9945..11294)
                     /gene="glmM"
                     /locus_tag="VFA02_09075"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZU10045.1"
                     /translation="MRRLFGTDGIRGVAGEAPLDPGTIYAVGLALAHQVKARNSHPRV
                     LLGMDTRESSAWIAAVLTAGLTEGGAKVESAGVITTPAIAYLARRYGFDAGVVISASH
                     NPWQDNGIKVFGADGYKLPDETELRIEEEIFRRLETVSAPDVSALSAPQVDEKYRKDY
                     EQFLLSAVPGLDLAGMKLVLDCANGAASAIAPELFSHLGGEIHLTHDSPDGRNINAKC
                     GALHPEIVAAETKSLQADMGVTFDGDADRALFADANGKVVNGDAVMLLAARDLHSRGK
                     LKGNVVVATTMSNMGLEAALRSNGIRMLRAPVGDKYVLERMQKEGASLGGEQSGHILF
                     PHLATTGDGLLTSLVVLDIVRRSGRPLHELVADLKVFPQVIVNVRVKEKKPLEEIATV
                     ADTIREAESSLAENGRVVVRYSGTEALARVMVEAESEDQMRHHAERIAGAIREALGA"
BASE COUNT         2438 a         3462 c         3021 g         2461 t
ORIGIN      
        1 tgttattttt gattacggaa tggtcctgag ccatcctcag gacccagccg cactcaacaa
       61 cattcttgcc attaccggac tcgaccgcga cacctttgac cggcattact ggacacaccg
      121 ccatgcctac gacatgggca aactgaacgg acgtacctat tggcaacaat ttgcccagga
      181 caccggcatc cggctcacac ccacggacgt tgaccgcctg gtcgaaaacg acgtgctcat
      241 gtggtgcacc atcaatgaac cgatgctggc gtgggccaaa tcactggccg aagcaggtct
      301 gcgcattgcc atcctctcca atatgggcga agagacgctg agctacatgc ggcaggagtt
      361 cgcatggctc ggcgatttcc atcatcacac ctggtcttgc gaactgggca tcgccaaacc
      421 agacccggcc atttacacct acacctgcga gaagctaggt gttactccca gagagacact
      481 ctttctcgat gacaagctgg aaaacgtaga ggcagcgcgc cgcgtcggcc tgaatgctct
      541 gcaattcagg aacatcgagc agctccggca cgatctggct tcccacggct ggcttgatgg
      601 cctgcccgtg cctgagaaga gtccgaaaac gtcacacaag cgaaatagca cattggcctg
      661 ttgcaatcgg gccaaggccc ttctttgttt tccgcagaag gggagggctg catatttctt
      721 tcttggggat tgacatttct ctcgacccta ttctagtcta ggtatgttta aaacaggcct
      781 atgtcccctt ttaaaaaatc cgaacctcgc gacctcttcc ccggcgcact ggagatgatg
      841 gtcctgcagt ctgtgcgccg gcagcctatg cacggatacg cccttgtcca gcacatcaaa
      901 cagcgctcga atgacctgct gcaggtagaa gaaggctcgc tttatcccgc tctgcaacgg
      961 cttctaaaag aaggactgct caaagcggag tggcagattt cccctacaaa ccgccgcgtg
     1021 cgaacatact ccatcacgcg cgccggcatc cggcacctcg aacacgaagt ctcgcgcttt
     1081 gagcagatgc tcgaaggcat tcacctggtc ctctccccta cagagcctta aaaggacaag
     1141 cctatgtttt cccgccgccg catttacgat gacctctctg aggaaatccg ccagcatctg
     1201 gaacaaaaaa ttgaatcgct gatggccgac ggcctcagcc gcgaagaagc cgagcatcag
     1261 gcgcggcggg agttcggcaa tgtcacttta atcgaagaac gcggccgcga gccgtggcaa
     1321 tggcctgggc tttattctct ctgggccgat gtgcgttttg gcctgcgcca gatcaggcgg
     1381 tctccctcat ttaccgtaat ggctgtgctc acgctggcgc tcggtattgg tgcaaacacc
     1441 gccatcttca cgctgattga ctccatcatg ctgcgcccgt tgccgtatcc gcaccaggac
     1501 cgcctcaccg tcctctcttc cggtggtgca tactttccca aaggctgggt tcgcgctctt
     1561 gcggaacatt cgcagtcttt ctcctccatc tccggctacg gccccaatgc cgaatcaaac
     1621 attgacgtgg gggactccgc cgagcgtgtc ttcggatcca gggtcagcgt caatgtcttc
     1681 gacacgcttg gcattcatcc tgaagcaggc agcttctttg ctccggaaaa cgccatcgcc
     1741 gggcaggacc aggtcgtagt cctcagcgac ggatatttta agcagcggtt cggtggaagc
     1801 cctctcgtaa ttggtcagac aattcgtatt gacggcatct cccgccgcat tgtgggcgtt
     1861 atgcctgccg gtatccattt cccttatgct gagacgcaat ttgtcattcc tgtttccttc
     1921 aaaggcggag acccttacga tccctgggta ggaccgtatg acctgcaggc aatcggcaga
     1981 ctcaaagacg gggccacgcc ctcatccgcc caggctgaat tgcgcagatt gcacccccag
     2041 ttactcaatc tttttccctg gcgcatgccg gatgactggg ccgcagaaat caacgtggtt
     2101 ccactactcg actttattgt cggggacaca cgtccgcgcc tgctgctcct ttttggcgcc
     2161 gtcggactca tccttctcat cgcctgcgcc aatgtcgcca accttatgtt ggcgcgcgcc
     2221 gcatcgcgcg agcgggaaat ggcgattcgc ggcgcgctcg gagcatccgg cagccgcatc
     2281 atccgccagt tgttgataga gagcggtctg ctggggatac tcgccggaat cgtcggattg
     2341 gttgcgtccg gcatcagcct gcgcacgttg acaagacttc tcccctcaga tacgccgcgc
     2401 attgccaata ttggactcca ctggaatgtc tactggtttg ccctgcttat atccgttctt
     2461 gccggggtcc tcttcggatt ggtccctgcg cttaagatgg catcgccaaa cctgcagcaa
     2521 tcattgcgct ccggcagcct gagcgttgtt ggaaaagggc cacaattcgg actttcgatg
     2581 cttctggtca ttgggcagat cgggctttcc gtcgttgtta tcacggcggc agggcttatg
     2641 ctgcgtagcc tttacagcct gtcacgcgtc aatcctggct ttcgtgtcag ccagattgtg
     2701 accgccgagg tctcgctgga cgccgatacg tgcccgtatc tggcatccca ggccgatggt
     2761 gagcggggcc gctgccaggc attcttccgt gaactgccgg accgcgcgcg tgccatccca
     2821 ggcgtcgaag acgcagcact ggcagatacg ttgcccatga gcggtaacga ccagatcttc
     2881 gcctatgatg cagaaggcca tcctcgcgaa gccagacagc aaccgcttct tgccatctcc
     2941 cgcgtggttt cgacagatta ttttcaaacg ctcggcctga agctgctgcg aggccgcctc
     3001 ctgaccggtt ccgaccagtc tggctcaagc cgtgctgttg tcatcaatca gaaaatggcc
     3061 gaagacctct ggcccaatca ggacccactc ggcaaacatg tcctgaatct cactgacgaa
     3121 aaaagccctg gggtcttcga tccgaatgtt gcttctatcg tggtcggcgt tgtaagcaat
     3181 acgcgccatc agaagcttgc tggagaccat tttcaggaag aaatctactt cccaatgacg
     3241 cccaaacttg agcagccatc catgcatatt cttttgcgcg ggcacgcaag cgtggaacaa
     3301 atcgcatcgg gactacggcg agtgattgcg cagctcaatc cccaggttcc ggtcacgcat
     3361 gtacgcacgt ttgaccaggt cattgctgct tccgatgccg ccccgcgctc gctcacgatt
     3421 ctgctgctca gcttcggcct tctcgccctc ggagtaggag gagcaggtgt ctacagcctg
     3481 attgcatata tcgtgagctg gcgcacgcgc gaaatagggg tacggctcgc tcttggcgct
     3541 tcacgccgtc agattgtgct catggtcctc aggcaaagcc tcatctgggc ggttgccggc
     3601 tcgctgacag ggctggttgc cgcggttgca tttgcacaga tgctgcggcg cttcctgttc
     3661 gaggtaagcc cgttcgatcc gcttacgatc agcttggttc cgcttcttat gcttctgatt
     3721 gcgctgctcg caacatggct gcctgcgcgt cgtgcagctt ccattgaccc tatgcaggcg
     3781 ttacgaatgg actaggaaga cagaaccgtc tgtgaaaatg ctatggccgc ttagtcccga
     3841 atcgtatggg ctgacgccag agtattggca gcggcgacac tgggtgtcac tggcgctgaa
     3901 ggggccgggg caggactcgt attcgccggg gccgtgctgc tggcatccag cgcgccagaa
     3961 tgatcagggc cgagacccag cttgatcaat gcgcgagaat cgggcaccag ctttgtctgc
     4021 cagccatggt ctgcctggta cttttgcatg gcggcctcag tctcctcgtc ccactgcccg
     4081 gaaggctggc cgctcaggta cttttcgcga atcagtgcct gctgaatttc ggtagcgcgc
     4141 tcgctgtcaa tgccgcgctg ccctatcacc tgatgcttca cggctttctt cttgctatgg
     4201 cctgatgtgg ctgcgcgatg gctccgtccg gcaaacgcag gagtggcaat aagcgccgca
     4261 gtcaataaaa ttgttgagca tgctcgaaca aaaagcacga tggcaaaacc tcgcaggggg
     4321 acttatctta cggtctttcc gaagagagta aatagtaaag cattctggtt gcaagcaaaa
     4381 caacaccggc atcctgtatt ttttgggatg ccggtgccac tttagtacag tgcattcccg
     4441 gttatggcaa agttccgcct accaggaaac tgccagcact tggacgctgc ggatccacga
     4501 cactcaacac aacatcctgc ccggatttca gaccggagac aattgcgttg aattccgcct
     4561 tgttatggac ctcctgcctg ttgatggatg taatgacctg gccttccata ccccccatat
     4621 tcagcacatt gtcagcaaaa gaacccggtt tgacccgctg aatcagcacg ccatgcaggt
     4681 tcggaggcgc attctgcggc agatcgctta ccgtgatacc cagcttgctc tgcgaggtat
     4741 ctacaccacc ttcaccgccg ccacctgggc cttgctcctc ggcctcgccg gaagcagctt
     4801 ccatcttgct gaagtcaatg atggaaaccg tggccgtgtg ctgctgtccg ttccgcagat
     4861 aaccaatctg cacagagtcg ccaggcttcc gggcagtaat ctggctgacc agatcatcgc
     4921 cgtccttcac cgggtggcca tccacgctta cgatgatgtc gttgggtttc agacccgcct
     4981 tgtcagccgg caatccaggc gtgacagcac tgatgatgac gccgttcttg ataccataca
     5041 cgcgctgcac agcgctggac acgttttgct ggaagcgaat gccaatggat ccgcggacca
     5101 ctttgtgttc cggcccgatg agcgcgttat aaaccccgat gactgtgttg gacggcatgg
     5161 caaaccctat gccctgatac cccgcggact gcgtaaagat ggcagtgttg acacctacca
     5221 cctctcctgc catattcacg agcgggccgc ccgagttgcc gggattaatg gcggcatcgg
     5281 tctgcagaaa gtgctgaaat tgtccttgcg ggccgccctg gatagtgcgg ttcttggccg
     5341 aaataatgcc cgccgtcacc gtctgctgga gcgtgaacgg gctgccaatc gcgatgaccc
     5401 agtcgcccac ctgagcagag tccgagttac ctagcttcac cgtaggcagg gggcggtcta
     5461 catcaatttt gatgacagca agatcggtgg ccttgtcagt gccaatcact tttgccggat
     5521 gtccctggtc gccttccggg tcgctggcca gctttacata gatgcggtcg gccttctcca
     5581 ccacgtgatt gttggtgatg atgtatccct tgggatcaac gatgaaaccg gagccaagcg
     5641 cctcatgcgc ctgatcatcg ggctgcccga aatcagggcc catcccgaag aagcggtgga
     5701 agaagtcctg cataccaccc tggtcgtcgt tgtcatcgtt gttatcatta ttgtcattgt
     5761 tgtcattgtt gtccgggttc tgcggattac gatggaaacg gcgaagccca cgctgctgtt
     5821 ccttgggcag ggtctctgtg ttgatgttga ccacagccgg cccgacttcc ttggcaattt
     5881 tcgtaaagtc tgtagaaagg tcgcgtggcg cggggacctt cagcggagtc gcatcggagc
     5941 tgtcaacttt cgcttcctgc ccgtggaccc catgcgaaac aacggagccg atgatgatcc
     6001 cggcggaaag tgtggcaagg acggtaaaag tagtcgtaag acggcgtaag cgaagccgtt
     6061 caagcaggga tttcattcga tctaacctcg tatcttttgg gagatgcaat cagaactgcc
     6121 ggcgcagtcg ccgtccaaca cttcagtata ccgaggaaaa tgaagcagat ggcgtgcgag
     6181 aaggcaggtt tcactttcta ttctcatcag gttagacgca aaatccggcc cggacgtcac
     6241 acggcaaaca aaggaagctc aggatttctc ttctcgaaca gcagccattt catgcgccgt
     6301 ggcaggcatc acagatggaa gcagctctgc gaaagtaatt ccacccagaa tgagaagcgc
     6361 acccatggac gcccgccagc tcagcctctc tcccagcacc acaaacgaag taagccatgc
     6421 aaataccggc tccaacgcca gaatcaacgc cgtgtgcgtt ggcggaagat actgctgcgc
     6481 atatgactga acagtaaagg ctgcggccgt ggcaagaatc gctgctacgc ccagggcaac
     6541 catgacgcgc cccgtccagt ggatccagaa gtgctccagc ataggcccgc tgatggccat
     6601 gaacaatgca cagaagccca cctgcagcac cgccagttgc gtaaatggca catggggcaa
     6661 cgcatgggcc agcgccaaaa catgcagcgc aaaccccagt gcgcagccaa gcgtaagcac
     6721 atcgcctgca ttcatcgaac tgaatccgaa tgtcacatga gcaggcgtag tcagcagtac
     6781 aattcccaga aacgctagca aggcccccag acaggcattc cagcccggca cacgggaatt
     6841 cgcagtccgc agaaacggaa ttgcagaaag cagcggaacc agcacgacca tcaacccggt
     6901 aagaaaggcc gatttcgatg gcgtggtaag gcgtagtccg gcggtttgaa actgatatcc
     6961 catcgcaagg cacagtccca ccagagctcc ataactaagc gcatgacgcg taaggtccct
     7021 cagatgcctg cggtataccg ttgccagaca ggcaaacgcc agtgccatgc gcagcaggtt
     7081 aaacaaaagc ggagagatgt cctgcagcgc atctttaatg agaacgaacg tcgctcccca
     7141 gacgaaaaca accacaatca atagcagatg ggcgcgcaga gagcgtgaca tggaagaaga
     7201 gtagcatcct gccacaattt actgcgtagg cttgctccac acttcgttga gaaattcata
     7261 ccgcacgcgt gtcttatcag gacgtgccgt tgattccgca ttcaggtgca tcacctgcca
     7321 tcctgtagcg gagctgtaaa cgggccactt tggcagtcca ggaccgttgg gatcacccgt
     7381 tttcgcaaaa tttgtccaat aactctgcat cagctcggac aattgcttgt cttccgggcg
     7441 caccatcatt cccttgcgtg aatcgagcgt gccgaaaacg tactcgatat catcggaatg
     7501 aaacgcagta ccgggagcat ggaacttgtc tccgggcgat ggcagctcaa agtggtaacg
     7561 atagaccggt tgtccgcctg tggcctgctg cgcttcaatc caccgccagg ttgcatagac
     7621 caggaagcgg tcgccggcgt agtcacgtag cgcgcgcagc gcctctgtac cgcttgccgc
     7681 ggcgtaagcc gacaggaact ggttcgcatg agcgccaaac tccttctgcg caaattggcg
     7741 gtactcagca atcgtcattg ccttctgcgg aatgtttcct tcgtcacggt tccatccagc
     7801 caacaatgga acatgcgcct gcttgcccgc cgcgtagatg gaagcaacat catccggcag
     7861 gaaataaccg tcaatgtccg gccagaaacg cgctgccggg tgcccgggcc gggaaacaac
     7921 cgccttcagc agatcatctg cgctcatggc cctcaggtcg ctcagctttg acgtgccaaa
     7981 tgccttctgc agcttttcaa catcctcttt ctcgcgttgc tcccggggca ggaacggctc
     8041 acgctgactg aaagccgcgc cactctcgcc aatggcgcga tgaatcaggt ctttcgacaa
     8101 gggcgatgcc atctgcgcgc tcacggaaaa tgacccggca gattcgccga agatggtgat
     8161 attgttcggg tctccgccaa agtttttgat attgcggcgt acccattgaa tcgccgccgc
     8221 ctggtccatc agaccgtaat ttcccgaagc gtggtgcgga gattccgccg tcagttcagg
     8281 atgcactagg aagccaaaga tgttcaggcg gtagttcatg gacacaacaa ctacgccttt
     8341 gtgcgccagg ttttccccgt cctgacgggc ctccgatgcc gccccggcat agaagccgcc
     8401 accataaatc cagaccatca ccggaagctt cgcattcttg tctttcgcag gtgtccacac
     8461 attcagattg aggcagtcct cacttgctcc cgggtcgcgg aagagcatgt ccgcaaaaac
     8521 actcgcctgc acgcagtggt gtccaaagtt cgtagcgtcc agcgtgccat gccattttgc
     8581 cgcgggttgc ggaggtctcc agcgcagcgg ccccaccgga ggcgcagcat acggaatacc
     8641 cttgaagatg cggacctttc catcggcgct gacgtatccg cgtaccttgc ccttgtccgt
     8701 tttcacagta gaggacttgt ccgcaaatga acccacgctt acggccgcca tacataccac
     8761 taaggctgtt cgcaaaacag tccgcatcag caaaaccctc tgcccttcaa aatatgttta
     8821 gaaaaaaggt atcacgtcag gcaggtaaaa taacctcgtg agcacttcca cggcaacatt
     8881 cgaggacatc cggctcaaag tccacgccgg ggaacgcatc tcgagcgaag aggccctctg
     8941 gctctggcgc aatgccagcg acggcgaatt gcgcggcctt gcctctgcgg tgcgcgcgcg
     9001 ctttcatgcg ccaaagtcct gcacatatat ggtgatgcgc atcatcaatt acacgaatgt
     9061 gtgcgtggcc cagtgcgatt actgcgcctt ctaccgcctg cccggagaca agcaaggcta
     9121 cgtcctctca caggaacagg tcttcgccaa actggatgag ttgcttgcgc tcggcggcga
     9181 cctcgcggcc tttaatggcg gattcaaccc acaccttcca ctcgattatt actgcggcct
     9241 gtttgccgcc attcgcgcgc gctacgggaa acggcttgag ttctacgccc ttaccatcgc
     9301 ggaattcatg tatctggccg accacgcgag gctcgattac gcgaccgccg cgcaacgctt
     9361 caaagatgca ggcgtgcgct ggattacagg tggcggatca gaaatcctga cggaagactt
     9421 tcgcagccgg cacagtaagt tcaaatacac cgtcgcagaa tactttgagg cacagcgcgc
     9481 catcatcgaa gccggactga acaccacagc aaccatggtc atcggctttg atgagacgat
     9541 agacgagcgc cttgagcatc tggagcgcac gcgccgcttt caggacgaaa ccaatggcct
     9601 cgccagcttt ctctgctgga cctacaagcc ttacttcact cagcttggag gaatcgaagt
     9661 cacgacggcg gaatatctgc ggcatctggc cctgtgccgt acttatctgg acaatgttcc
     9721 gcgcatccgc acctctgtcc ttacgcagaa tgaacgcgca ttagaggggc ttctctacgg
     9781 tgccgacgat ttcgacctgc ccatcgaaga tgaagtcacg cagaaagccg gagcgaccat
     9841 cagccttgag ttcgacaaga ttctggatgt agcgcgacgg ctgggatttg accccagcta
     9901 ccggagcgtg gcggcgacaa ccttataagc tgtccttctg ctcatcacgc cccgagtgct
     9961 tcccgaatcg ccccggcgat cctctccgca tgatggcgca tctggtcttc cgattccgcc
    10021 tcgaccatca cgcgcgccag cgcctccgtt cccgaatagc gcacgactac gcgtccgttc
    10081 tcagcaagac tcgattcggc ctcgcgaatc gtatctgcca cggttgcaat ttcctcgagc
    10141 ggcttcttct cttttacgcg tacattgacg atgacctgcg gaaacacctt cagatcggcc
    10201 accaactcgt gcagcggtct tccggagcga cgcacaatat cgagcactac aagcgaagtc
    10261 agcagtccat cacctgttgt cgccaggtgc ggaaagagaa tgtgccctga ctgctcgccg
    10321 ccaagagatg cgccttcctt ctgcatgcgt tccagcacat acttatcgcc caccggagcg
    10381 cgtaacatgc ggatgccatt gctacgcagt gccgcttcca ggcccatgtt ggacatggtg
    10441 gtggccacaa ctacattgcc cttcaatttt ccgcgagagt gcagatcgcg cgcagccaaa
    10501 agcatgactg catcgccgtt gaccaccttc ccgtttgcat cagcaaagag tgctcgatcg
    10561 gcatcgccat cgaaggtcac gcccatgtct gcttgcagcg actttgtctc cgcggcaaca
    10621 atttcagggt gcagcgcgcc gcattttgca ttgatgttgc gtccgtccgg cgaatcatgg
    10681 gtcaggtgga tttcaccgcc cagatgggaa aacaattccg gcgcaatggc agaagccgcg
    10741 ccattcgcgc aatcgagcac cagcttcatt cctgcaagat ccagcccagg aacggccgaa
    10801 agcagaaact gctcatagtc tttgcgatat ttctcatcca cttgcggagc ggaaagcgca
    10861 gacacgtccg gcgcagaaac ggtttccagc ctgcgaaaga tctcttcctc gatgcgaagc
    10921 tccgtctcat cgggcagctt gtagccatct gcgccgaaga ccttgatgcc attgtcctgc
    10981 catggattgt gcgaagcaga gatgaccacg cccgcatcaa agccatacct gcgcgccaga
    11041 taggcaatcg cgggagtcgt gatcactccg gcactttcaa cctttgcgcc gccttctgtt
    11101 aatccggccg tcagaacagc ggcaatccat gcactcgatt cccgcgtgtc catgcccagc
    11161 aaaacccgcg gatggctgtt ccgcgccttc acctggtgcg cgagcgccag cccaacagca
    11221 tagatcgttc ctggatctag cggcgcttct cctgctacgc cgcgaattcc atccgtacca
    11281 aatagtcttc tcatttcgta gttgttcttt ccggaaaaat ttctgtcacg cggcccacca
    11341 cacgcccatc ggccagacgg gccgtcatct gctgtcctgc ac
//
LOCUS       DASRBV010000243         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_623862, whole genome shotgun sequence.
ACCESSION   DASRBV010000243 DASRBV010000000
VERSION     DASRBV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623862"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1118
                     /gene="rodA"
                     /locus_tag="VFA02_09080"
     CDS             18..1118
                     /gene="rodA"
                     /locus_tag="VFA02_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZU10046.1"
                     /translation="MRRFLSFRDFDWTLLGFVMALSIISILEIYSATLHTKFVGFHKM
                     QILWLLGGLAAMFVLSVIDYHRLLEWIHWIYGFCVVSLIAVLAVGTKVLGARRWIKLP
                     GGIHFQPSEWVKLVLIVAAARYFANLAGKRLTWPDIFKAIAMVGVPMLLVLKQPDLGT
                     ALTYSPVLMVGLFLGGIDWKKAGTIILVCLLGAGVVWKTGKILKPYQKARLTSFMNPD
                     ADPRGSGYQILQSKIAVGDGGVFGRGATKGTQTQGDFLPIPYTDFIFAAYCEEHGFIG
                     AMIVLLLYFLILMRLIQNAQTAADLPGTFIVMGVVAVLIFQIAVNIGMVIGLMPVTGI
                     PLPLMSYGGSSVLFTFLALGMVMNVRMSRFVN"
     gene            1443..4274
                     /locus_tag="VFA02_09085"
     CDS             1443..4274
                     /locus_tag="VFA02_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZU10047.1"
                     /translation="MSKEICISSTPHETRLAILENDQLAEIYYERENEYTLAGSIYKG
                     RVTRVLPGMQSAFVDLGLERDAFLYVTDFLEEQEDSADFERIGGPPQQRAEEKGRAPQ
                     VQEEEQGPEEPSEDAPEGTRRWKGRRGRRGREHRPQQQAHEGNGHHIESPEAVEESIA
                     AEPTPAPEHGAPGGIPAAHFPVLLPGESISKYGGKPASSEAAKAAAPRAVYATSRPST
                     LVEGPIEWTGGDLLPGESRAWRSSQTEQGVAAGEAAAIVPEVPEIHQQEATEAFPEAV
                     TPETEHAEAPETALSETVEEVSNRPEVVQEAAEEHHEQVEHVEEVHEYEPEHAEASHR
                     VDVAQPREFRISGTEIDEEPAPAQEEHVVPGTVTEEAAVTQPEYEVEYQEEEPQAAPE
                     PAAAEEGISHHAHAFAPGAGLLEEEVIDEEEYDFEPLQAHAEEQDFEDLEEETLQHEV
                     NGTDLGAMVRDLHINQRVNFGPEGALAQETEEEEYAAEAVEAEDIEEEVEEEETEERP
                     QDQGRVQRERRRGRGRHGRRRHTGGMRRATAQVTDLPAITELLKPGQEILVQIAKEPI
                     AKKGARITSHIALPGRFLVFMPTVNHVGVSRKISSDEERYRLKRILISEKGTASGGFI
                     VRTAAEGASEEELRADLRFLINLWNDIKHRSDEAKAPALIYHDLNLVERVLRDQVSES
                     FSTIWVDSETEYERVVRFLNRFQPSLVRRVKLYTKETPLFEQFGIQDEISKALKSKVW
                     LKSGGSIVINQTEALVAIDINTGKYVGKTARLEDTILKTNLDAIPEIVRQIRLRDLGG
                     IIVIDFIDMDERKNRQKVMQALEEELKNDRAPSKVLQFNDFGLVAITRKRVKQSLERT
                     LSVPCYICAGTGMVKGPVTVCNEIYIEMRKMQKHLERNDVLLRVHPEVVKTLKANNAR
                     WLTEMEEMTGKTIIVKSDPTIHPEQFDIH"
BASE COUNT          992 a         1109 c         1334 g          847 t
ORIGIN      
        1 ataatagaaa gtagttcatg cgccgttttc tctcgttccg cgatttcgac tggaccctgc
       61 ttgggttcgt catggcgctt tccatcatca gtattctgga aatctacagc gctacgctgc
      121 acacaaaatt tgtcggcttc cacaaaatgc agattctctg gctgctgggt ggactggcgg
      181 caatgtttgt gctttccgtg attgactacc accgccttct ggagtggatc cactggattt
      241 atgggttctg cgtggtttcc ttgattgctg tgttggctgt gggtaccaaa gtgctggggg
      301 cgcggcggtg gatcaaactg ccgggaggca tacatttcca gccatcggaa tgggtaaagc
      361 tggtgctgat tgtcgcggcg gcgcgatatt tcgccaatct tgcgggcaag cggcttacct
      421 ggccggacat ttttaaggcc atagccatgg tgggcgttcc gatgctgctg gtcctgaagc
      481 agcctgatct ggggacggcg ctcacatact ctccggtgct gatggtcggg cttttcctgg
      541 gtggaattga ctggaaaaaa gccgggacca tcatcctggt ctgcctgctg ggagcaggag
      601 tggtctggaa gacgggaaaa atcctgaagc cataccagaa ggcacggctt accagtttta
      661 tgaatccgga tgcagaccca cgcggttccg gatatcagat tctccagtcg aagattgccg
      721 tgggcgatgg cggggtgttc gggcgtggtg cgaccaaagg cacgcagacg caaggggatt
      781 ttctacctat cccgtatacg gactttattt tcgcggcgta ttgcgaggaa cacgggttta
      841 tcggcgcgat gattgtcttg ctgctatact ttttaatatt gatgcgtttg attcaaaacg
      901 ctcaaacagc cgccgacctg ccgggcacgt tcatcgtaat gggcgtggtg gccgtgctga
      961 tttttcagat tgcggtcaat atcggcatgg tgatcgggct gatgccagtc accggaatcc
     1021 ctttaccatt aatgagttat ggggggtcgt cggtgctgtt cacgttcctg gccctgggca
     1081 tggtgatgaa cgtccgcatg agccgcttcg tgaactgaag gtttgcaaag gtttgtaagg
     1141 ctttcgcctg ttcgcgaaag ccggtaagga agttaagaaa tttaattatt agccggcctg
     1201 aggcggatcg ccctgccggt caggaccaag gaatgagcgc gctgagacgg gtaacggccc
     1261 cgccgaacag attggctgaa gcggtcacaa aatctctgcg cccagatagc cttcccgaaa
     1321 tcaaccaaaa tttttacgga ccacgacggt ccctggaact ctggtggaat acggccatct
     1381 cattgtcatc tctggtctcg tccggttttc cggtgtgatc tgcctcggct gaaagaggca
     1441 gcatgtcaaa agaaatttgc atttccagca cgccgcatga gacgcggctt gcgattttag
     1501 aaaatgacca gctcgcagag atttattacg agcgggaaaa tgaatatacc ctggccgggt
     1561 caatctataa aggccgcgtg acgcgcgttc tgcccggaat gcagtcggcc ttcgtggacc
     1621 tcggactgga acgcgacgca ttcctttacg tcacagattt cctcgaagaa caggaagatt
     1681 cagcggactt tgagcgcatt ggcgggccgc cacaacagcg ggcggaagaa aagggccgcg
     1741 cgcctcaggt ccaggaagag gagcaggggc cggaggaacc gtcggaagat gctcctgaag
     1801 gcacacggcg ctggaagggc cgtcgtggac gtcgtggacg ggagcaccgt ccgcagcagc
     1861 aggcgcacga gggaaacggg caccacattg agtctccgga agcggtagag gagagcatcg
     1921 cggcagagcc gaccccagcg ccggagcatg gcgctccggg aggaattcct gcggcgcatt
     1981 tccctgtgct gctgccggga gagtccattt caaaatacgg cgggaaacct gcttcttctg
     2041 aagcagcgaa ggcggctgct cccagggcgg tctatgcaac ttccagacct tccactctgg
     2101 tggaaggccc gattgaatgg acgggaggcg atctgcttcc cggcgaatcg cgcgcatggc
     2161 gctcctcgca gacagagcag ggcgttgctg cgggagaagc ggctgcgatt gtgccggaag
     2221 tcccggaaat tcatcagcaa gaggcgacag aagcctttcc ggaagcggta acaccggaaa
     2281 ctgagcacgc agaggcaccg gaaacggccc tcagtgagac ggtcgaagag gtttcaaaca
     2341 ggcctgaagt tgttcaggaa gccgctgagg aacaccacga gcaggtggag cacgtcgaag
     2401 aagtccacga atatgagccg gaacacgccg aagcctcgca ccgtgtggat gtggcgcagc
     2461 ccagggaatt ccgtatctcc ggcaccgaga tagatgaaga acccgcacct gctcaggaag
     2521 aacacgttgt tcctggaacc gtgaccgaag aggccgctgt cacacaacct gagtacgaag
     2581 ttgaatacca ggaagaagag ccacaggctg ctcctgagcc agcggccgct gaagaaggga
     2641 tttcgcatca cgcgcacgca ttcgctccag gggcgggact tctggaagaa gaagtgattg
     2701 acgaagagga gtatgacttc gagccattgc aggcacacgc tgaagagcag gacttcgagg
     2761 acctggaaga agagacgctc cagcatgagg tcaacggcac agacctcggc gcgatggtgc
     2821 gcgacctgca tatcaatcag cgtgtgaact ttggacccga aggcgctctg gcgcaggaga
     2881 ccgaagagga agagtatgct gccgaggcgg tggaagcaga agacatcgaa gaagaggtag
     2941 aggaagagga gacagaagag cggccccagg accaggggcg cgtccagcgg gaacgccgcc
     3001 gtgggcgtgg acgccatggc cgccgccggc acacgggtgg aatgcgccgc gctacagcgc
     3061 aggtcaccga tcttccggcc attaccgaac tgctgaagcc cgggcaggaa attctcgtcc
     3121 agatagccaa agagccgatt gccaagaagg gcgcgcgcat taccagccac atcgcactgc
     3181 ccggacgctt tctggtcttt atgccgacgg tgaaccacgt aggggtgtcg cgcaagattt
     3241 cttccgatga ggaacgctat cgcctgaagc gcattctcat cagcgaaaag gggactgctt
     3301 ccggcggatt cattgtccgc acagcagcgg aaggcgcgag cgaagaagag ttgcgcgctg
     3361 atctgcgttt ccttatcaat ctgtggaacg acatcaagca ccgctcggac gaggccaagg
     3421 ccccggcgct gatctaccac gacctgaacc ttgtagaacg cgtgctgcgc gaccaggtga
     3481 gcgagagctt ttcgaccatc tgggtagaca gtgagaccga atacgagcgc gtggtgcgct
     3541 tcctcaaccg cttccagcct tcgctggtgc ggcgcgtgaa gctctatacg aaagagacgc
     3601 cgctcttcga gcagttcggc attcaggatg aaatctcgaa ggcgctgaag tcgaaggtat
     3661 ggctcaagtc cggtggctcg attgtcatca atcagactga ggcgctggtt gcgattgaca
     3721 tcaacaccgg caaatacgtg ggcaagacgg cgcgactgga agacacgatt ctcaagacaa
     3781 acctcgatgc gattccggag attgtccggc aaatccgtct gcgcgacctc ggcggcatca
     3841 tcgtgattga cttcattgac atggacgagc gcaagaaccg ccagaaagtg atgcaggcgc
     3901 tcgaagagga gttgaagaac gatcgcgctc cgtcaaaggt gcttcagttt aatgatttcg
     3961 gactcgttgc catcacgcgc aagcgtgtga agcagtcgct ggagcggacg ctgagcgtgc
     4021 cctgctacat ctgcgcgggc acaggtatgg tgaaggggcc ggtgacggtc tgcaacgaga
     4081 tctatatcga aatgcgcaag atgcagaaac atctagaacg caatgatgtg ctgctgcgcg
     4141 tgcatccgga agtggtgaag acactgaagg caaacaatgc gcgctggctg acggagatgg
     4201 aagagatgac gggcaaaacg atcattgtca agagcgaccc gacgattcat ccagagcagt
     4261 ttgacattca ctgaagcatt tt
//
LOCUS       DASRBV010000244         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_165592, whole genome shotgun sequence.
ACCESSION   DASRBV010000244 DASRBV010000000
VERSION     DASRBV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165592"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFA02_09090"
     CDS             complement(<1..812)
                     /locus_tag="VFA02_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZU10048.1"
                     /translation="MMKASRHMVFLSVVSLAAFFAVAVTVDVHWKSGLLQWRQERARQ
                     LSAPDGWLALVGLEWLKPGPNSFGSAAANRIRLNAPVDAHLGVLELKGNDVYLSSPAP
                     SLLENGAPAQPGKIETDGENPTVLKAGTLTLLVIHRGDRFALRIKDSQAPTRIQFKGL
                     HWYAPDARYRITAKWLPFNPPHEETIPTIIGTTLKLPVPGVAEFTLDGQTVQLEPVLE
                     GPEEKKLFFILRDATSKTTTYGAARFLYTDFPDHGLDKPGHVVLDFNRLQNPP"
     gene            1148..2056
                     /gene="panB"
                     /locus_tag="VFA02_09095"
     CDS             1148..2056
                     /gene="panB"
                     /locus_tag="VFA02_09095"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZU10049.1"
                     /translation="MSVTNFCSADAQNASAIRAKVTFQTLQEKKQSGRPITALTAYDY
                     ATARLVDEAGVDLILVGDSLAQVVLGYDTTLPVTVDEMLHHTRAVRRAVKHAIVAADM
                     PYGSYQMGVQDGVTNAVRFMKEAGAEAVKIEGGANRAELVERLADAEVPVIGHLGLTP
                     QSVHRMGGYKVQGKSLSAADTLLKDALALESAGAVLIVLEGMPREVAQHITQRLNIPT
                     IGIGAGPDCDGQILVFHDLVNLSFGKPAKFVRRYGDAATLFRSAIDGYVRDVERRDFP
                     SEDESYHMPKEVRGAFEASVPLRRKA"
     gene            2111..>2685
                     /gene="panC"
                     /locus_tag="VFA02_09100"
     CDS             2111..>2685
                     /gene="panC"
                     /locus_tag="VFA02_09100"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZU10050.1"
                     /translation="MLIFQSIQEAQSACAQLRGEGRILGFVPTMGALHEGHLSLVRAA
                     KAECDLVAVSIFVNPTQFGPNEDFAKYPRTFEQDCALLEREGVHLVFAPTAEGMYPTG
                     ASTFVHVEGVSERLDGASRPGHFRGVATVVSKLFHIVCPHKAYFGQKDAAQVAVLRKM
                     VRDLNFPVQLVVCPIVREPDGLAMSSRNRYLS"
BASE COUNT          594 a          820 c          732 g          539 t
ORIGIN      
        1 ggaggattct gcagccggtt gaaatcgagc accacatggc ccggcttgtc gagcccatgg
       61 tcaggaaaat cagtatacaa aaagcgcgct gcgccatacg tcgtcgtctt gctggtcgcg
      121 tcgcgcagaa tgaaaaagag cttcttctct tccggccctt ccagaacagg ctccaactgc
      181 actgtctgcc cgtcgagcgt gaactcggcc actccgggca ccggcagctt cagcgtcgtg
      241 ccgatgatgg taggaatcgt ctcctcatgc ggaggattga agggaagcca cttcgccgta
      301 atgcgatagc gcgcatcggg cgcataccaa tgcagtccct tgaattgaat gcgcgtcggt
      361 gcctgcgagt ccttgatgcg cagcgcaaag cggtcgccgc gatgaatgac cagcaacgtc
      421 agcgttcctg ccttcagcac ggttggattc tcgccatccg tctcaatctt tccgggctgc
      481 gccggtgcgc cgttctccag cagcgatggc gctggcgacg acagatacac atcgttgccc
      541 ttcagttcca gcacacccag atgcgcgtca acaggcgcat tcaaccggat gcggttcgca
      601 gctgccgagc caaaggaatt ggggcccggt ttcagccact ccaacccaac cagcgcgagc
      661 cagccatcgg gagcagagag ttgtctggcc cgctcttgcc gccattgcag aagcccactc
      721 ttccagtgga catccaccgt taccgccaca gcaaagaacg ccgccagaga cactaccgaa
      781 agaaaaacca tatgccgaga ggctttcatc atggccctcc ttaaaaaaga agagtgaata
      841 aaaatgagtt gtagaaatga cgcagaattc agcgacaaca gcagcgcggg ccagaagatg
      901 gaaaagaagc aaaccagaga atttgcatca tgcgtccttc tattgtgcct caaccgcgta
      961 aattccggtt atatttttta tccgtcaaaa tgcgctggcg aatctccggt tatttttttc
     1021 gtggtcgcgc ctcccattgc acagcgcaaa tcaggacctg ggttgacttg tgcgcacatg
     1081 ccttcaggcg tatagtcgga gatgttcggg tagcatccct tccatgggag ctgcactgga
     1141 gacaaccatg agcgtgacca acttctgtag cgcggacgcg caaaatgcct cggccattcg
     1201 cgccaaggtg accttccaga cacttcagga aaaaaagcag agcggcagac ccattactgc
     1261 gctgactgcc tacgactatg ccaccgcccg tctggtagac gaagccggag tagacctcat
     1321 cctcgtcggc gattcgctcg cgcaagtcgt cctcgggtat gacaccacac tccccgtcac
     1381 cgtagacgag atgctccacc acacccgcgc tgtgcgacgc gccgtaaaac acgccatcgt
     1441 tgccgcagat atgccctacg gctcctacca gatgggcgtg caggacggcg tgaccaacgc
     1501 ggtccgcttt atgaaggaag ccggcgctga agccgtcaaa attgaaggcg gagcgaaccg
     1561 cgccgaactg gtcgagcgtc tcgctgatgc ggaggtcccc gttatcggcc acctcggcct
     1621 tacgccgcaa tccgtgcatc gcatgggcgg atacaaggtc cagggcaagt cgttaagcgc
     1681 agctgatacc ctgctcaaag acgcgctcgc gcttgaatcc gccggcgctg tgctgattgt
     1741 gctggaagga atgccgcgcg aagtggcgca acacatcacc cagcgtctga acattcccac
     1801 catcggcatc ggagcaggcc cggactgcga cggacagatc ctcgtctttc atgatcttgt
     1861 aaacctcagc ttcggcaaac ctgcaaagtt tgtccgccgg tacggagatg ctgccaccct
     1921 cttccgctcc gccattgacg gttacgtgcg cgacgtggaa cgccgcgatt ttcccagcga
     1981 agacgagagc taccatatgc ctaaagaagt gcgcggtgca tttgaagcat cggtaccgtt
     2041 gcggcgcaaa gcctgaacga agccgaaggc acagtcaaaa atctggacca tctctgcact
     2101 ggaatccatc atgctcatct ttcaatccat tcaggaagca caatccgcat gtgcccagtt
     2161 gcgcggagaa ggccgtattc ttggctttgt ccctaccatg ggcgcattac atgagggcca
     2221 tctctctctg gtgcgcgccg caaaagccga atgcgatctc gttgcagttt ccatctttgt
     2281 gaacccaacg cagttcgggc cgaacgagga cttcgcaaaa tatccgcgca cctttgagca
     2341 ggactgtgcc ctgctcgaac gcgaaggcgt gcatcttgtc tttgcaccca cagcagaagg
     2401 gatgtatccc acaggggcca gcacctttgt tcacgtcgag ggcgtaagcg agcgcctgga
     2461 tggcgcttcg cgtccagggc attttcgcgg agtggcgacc gtcgtcagca agctctttca
     2521 catcgtctgc ccgcacaagg cttacttcgg gcaaaaggac gccgcgcagg tcgccgtcct
     2581 gcgcaaaatg gtgcgtgatt tgaattttcc cgtgcagctt gtcgtctgcc ccatcgtgcg
     2641 cgagccggac ggcctcgcca tgagctcccg caatcgctat ttatc
//
LOCUS       DASRBV010000245         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_70081,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000245 DASRBV010000000
VERSION     DASRBV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70081"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..2299
                     /locus_tag="VFA02_09105"
     CDS             50..2299
                     /locus_tag="VFA02_09105"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZU10051.1"
                     /translation="MATAQSASVQPVPVQAPQDSFAPQFKKLIATVRANRPNDDTDII
                     RKAWEFCLEHHKGQTRASGEPFVLHPLEVAVVLAEMKLDSTAIAAGLLHDAVEDTPVT
                     TAEIASKFGEQVAHIVEGVTKIDKIQFANKEDRQAENVRKMLLAMVSDVRVVLIKLAD
                     RLHNMRTLEHLPPEKQQVIARETLDIYAPLAHRLGMGKVRGELEDLAFRYVDPYAYQQ
                     VHDAVEARRSEGEQFLAGVEALLQEKLRENNIQARMEWRIKRLYSIHQKLVKQRITVD
                     QVYDLLALRVITNSVQDCYAVFGLIHSIWRPVPGRIKDFIAMPRPNLYQSLHTTVMGE
                     GGHQFEVQIRTEEMHRIAEEGIAAHWKYKDGSPISARDEQRLAWVRQLVEWQREMSDP
                     NEFLSTLKIDLYPEEVYTFTPKGKVVVLPKDATPLDFAYSIHTEVGHSCVGAKVNGRM
                     VPLRSRLRNGDIVEVITQNGHTPSRDWLTFVKSSRARNKIKHWLNEHQRERAVEIGKK
                     LLEREARKYKVSLAKFEESDYERVAQDYSMATPNDLLASVGFGKYSARQVLNRLAPGS
                     TAQQVPETEAAVSSAGVSETVKRVSRASADSLQVEGQNELLVYRARCCNPIRGEEIIG
                     YVTRGKGVAVHARSCPNVQNLLYESDRRISVEWARTAEEAQGRKTTYPVRLTVYCDDR
                     AGMLKEMTAVISDDNTNIRSVDSKPGPNNDATVEFVVEAEDVRHLNRLVQGLKRVPGV
                     RDVQRSQKL"
     gene            2486..>5415
                     /locus_tag="VFA02_09110"
     CDS             2486..>5415
                     /locus_tag="VFA02_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10052.1"
                     /translation="MQGSCPRKWYRYVFAVWLLLVVVHLSAQVNTASLTGLITDPSGA
                     AIPNVTVKATNAATGYERTVQTDNAGYYSFQNLPIGQYTVRVEASGFNAVEENVTLNV
                     AEKGRRDFSLQVGSAQQTVEVQAQAQGLSPDDASIGTVISSHTIEQTPLYLRNWDDLL
                     RTVPGVQISRYTQQSGATSAGRVGDFNVNGVHSLQNNFILDGIDNNTFSENVQELSTE
                     AAHPSVDVIAEFNIITNPYSAEYGRAPGAVVSVNTRGGTNEIHGLAYEYVRNQYFDAT
                     DFFTKQHGQRKPENNQNQFGGSLGGPFIKNRLFGFFNYEGTRIKQGVTRTSTVPLPNE
                     RTGDFSPAASARTGIKYPTIYDPSTGQPFQNNQIPSTRIDPVVANLIALFPLPNVTVN
                     GLTPETNNYFRNAIAEDNTDSYDGRVDWTPSSNDTIFGRYNYSNRFRYIPGYLGGLAD
                     GSSTSAWGRQKLKNHSFVLGWTHIFSPSLVNDFRFGWVRNYAFAEQDPFALTQYAGNF
                     VPGIPINPAVGGGVPLTTFSGSIGAFLGSPDFLPKQQVPQQFQYNDTLSLTHGTHTFK
                     VGASLYLPMRNLFQDEPGMRGDLTFNGQFTCQRNASSQCVSGTGFPYADGLLGLVQSN
                     QLTNVHFVDQRLWMLSGFFEDDWKVTPKVTLNLGLRYEFATPALNAKDQMANFDPIAG
                     ALVFARSGGLKSRALVNPNYKNFGPRIGFAYSPDQKTVLRGGYGIYYTVFERYGSEDQ
                     LSLNPPYLINKTQSAQSTSTTPVMIAQQGFPSNYLDPSTINLSNLTAFHIRAMNSNDP
                     TPYVQQWSFGIQRAIGNTWTAEVDYVGTKSTHLDIIRDYNQPLISGNTVQTTKNSAGQ
                     VVPVIPYSNFGQIEYTDAIGYGNYNGLQASLKRRFQNGLSIQTAYTYSHSLDDVPEEL
                     ESNSGDAPNGRNPSMWYGNSDFDIRNRVSVSYVYELPFGHGKPMLNHGALSWILGDFR
                     TSGV"
BASE COUNT         1247 a         1549 c         1436 g         1183 t
ORIGIN      
        1 gaggtatcgc gttttatacg aaatttttca cctcggcata taatcagata tggcaactgc
       61 gcaaagcgca tcggtccaac ccgtaccggt ccaggctcca caggactcct ttgcgcctca
      121 gtttaagaag ctgattgcca ctgtccgggc gaaccgtccc aacgatgata cggacatcat
      181 ccgcaaagcc tgggagttct gcctggagca ccataaaggg cagacccgcg cctcgggcga
      241 accgtttgtg cttcatccac ttgaggttgc ggtcgtcctg gcggaaatga agctggactc
      301 gaccgccatt gcggctggcc ttctgcacga tgcggttgag gacacgccgg tcactacggc
      361 agagattgct tccaaattcg gcgagcaggt ggcgcatatt gtcgagggcg tcaccaaaat
      421 tgacaaaatc cagtttgcca acaaggaaga ccgccaggca gagaacgtcc gcaagatgct
      481 gctggccatg gtcagcgatg tgcgcgtcgt cctgattaaa ctggccgacc gcctgcacaa
      541 tatgcgcacg ctagagcacc ttccgccaga gaagcagcag gtgattgcgc gcgagacgct
      601 cgatatctat gcgcctcttg cacaccgtct gggaatgggc aaggtgcgcg gcgaacttga
      661 agacctcgcc ttccggtatg tagaccccta cgcttatcaa caagtgcatg atgccgtgga
      721 ggcgcgtcgc agcgagggcg aacaatttct ggccggggtg gaagcactgc ttcaggaaaa
      781 actgcgcgaa aacaacattc aggcgcgcat ggaatggcgc atcaaacgcc tgtacagcat
      841 tcatcagaag ctggtgaaac agcgcatcac tgtagaccag gtctatgacc tgctggcgct
      901 gcgcgtcatc actaacagcg tgcaggactg ctacgccgtc tttggcctga ttcacagcat
      961 ctggcggcca gtgcccggac gcatcaagga cttcattgcc atgccgcggc ccaatctgta
     1021 tcagtcgctg cacaccactg tgatgggtga aggcggtcac cagtttgagg tccagatccg
     1081 caccgaggaa atgcaccgca tcgctgagga aggcatcgca gcacattgga aatataagga
     1141 cgggtcgcct atcagtgccc gtgatgagca gcgcctggcc tgggtgcggc agctcgtcga
     1201 gtggcagcgc gagatgagcg atccaaatga gtttctttcg acgctcaaaa ttgatctgta
     1261 tccggaagag gtctacacct tcactccgaa gggcaaagtg gttgtccttc ctaaggacgc
     1321 gacgccattg gactttgcct attccattca cacggaagtg ggtcatagct gcgttggcgc
     1381 aaaggtcaat gggcgcatgg tgcctctgcg gtcgcgtctg cgcaatggcg acattgtcga
     1441 agtcatcacc cagaacggac atacccccag ccgcgactgg cttacgttcg tcaaaagctc
     1501 gcgcgcgcgc aacaaaatca aacactggct caatgagcac cagcgcgagc gtgcggttga
     1561 gattggcaaa aagcttctgg agcgcgaggc gcgtaagtac aaggtttcac tggccaagtt
     1621 tgaagaatct gattacgagc gcgtggccca ggattacagc atggccacac ccaacgatct
     1681 tctggcttcg gttgggttcg gaaagtattc tgcgcggcag gtgctgaacc ggctggctcc
     1741 cgggtctacg gcgcaacagg tccctgagac ggaggcggcc gtctcttccg ctggagtatc
     1801 tgagaccgtc aagcgtgttt cccgtgccag tgctgattcc ctgcaggtgg aaggccagaa
     1861 tgagctgcta gtctatcgtg cgcgatgttg caatcccatt cgcggtgaag agattattgg
     1921 ctatgtgaca cgcggtaagg gtgtcgctgt acacgcacgg agctgtccga atgtacagaa
     1981 cctgctctat gagtctgacc gccgtatctc tgtcgagtgg gcacgcacgg cggaagaagc
     2041 gcagggccgc aaaacgactt atcctgtacg cctgacggtg tattgcgatg atcgcgccgg
     2101 aatgctcaaa gaaatgactg ctgtcatcag cgacgacaac accaatatcc gttcggtaga
     2161 ttcaaagcct ggaccgaata atgacgccac cgttgaattt gttgtggaag ctgaagacgt
     2221 gcggcacctg aaccgccttg tacaggggct gaagcgcgtc cccggcgtgc gtgatgtaca
     2281 gcgatcacaa aagctgtaga gtttcctttc aggttccttc ctcagcaatt tcataccgta
     2341 ttcatcttgt ctttaccgga ctttcagagt tactctttaa cgtcttcata tctgcgtaat
     2401 ttttcgcatg tttcttatat gcgcacgtat tcaatgttct cttcgtgcgt aaaaatcagt
     2461 tcttttccct ttgaggaggc gtagaatgca agggtcttgt ccccggaaat ggtatcgtta
     2521 cgtttttgcc gtatggctgc tgctcgttgt tgtccatctt tctgctcagg tcaatactgc
     2581 cagtctgacc ggactgatta cagacccgag cggcgcagcc attcctaatg taactgtaaa
     2641 agctacaaat gcagccacgg gctacgagcg cactgtgcag accgataatg ccggctatta
     2701 ctcgttccag aacctgccga ttggccagta cacggtccgc gtcgaggcct ccggattcaa
     2761 tgcggtcgaa gaaaatgtaa ctctgaatgt tgcagaaaag gggcgccgcg atttttcgct
     2821 ccaggtaggc tcagcacagc aaactgtgga ggtacaggcg caggcgcaag ggctttcacc
     2881 tgatgatgct tcgatcggca ctgtcatcag ctcgcacacg attgagcaga caccgcttta
     2941 tctgcgcaat tgggacgacc tgctccgcac cgtgcccggc gtacagattt cgcgctacac
     3001 gcagcagagt ggcgcgacct ctgccgggcg cgtgggcgac ttcaacgtca acggtgtcca
     3061 ctcactgcaa aacaacttca ttctggacgg cattgataac aacaccttct cagaaaatgt
     3121 gcaggagttg agcactgagg cggcgcaccc ctctgttgac gtgatcgcag aattcaacat
     3181 catcactaat ccatattcgg ctgaatacgg acgcgcgcca ggggccgtcg tatctgtaaa
     3241 taccaggggc ggaacgaacg agatccacgg cctcgcatat gagtatgtgc gcaaccaata
     3301 ctttgacgcg accgacttct tcaccaaaca gcacggtcag cgcaaaccgg aaaacaacca
     3361 gaaccagttt ggtggcagcc tcggcggccc atttatcaag aaccgccttt ttggcttctt
     3421 taactatgaa ggcacacgca tcaagcaggg tgtaacacgt acctcgactg tccctttgcc
     3481 gaatgaacgc accggcgact tcagccctgc agccagcgcc agaaccggaa tcaaatatcc
     3541 gaccatttat gaccccagca cgggccagcc tttccagaac aatcaaatcc cttccacacg
     3601 gattgaccct gtggtcgcaa atctgattgc gctttttccg ctgcccaatg tcacggtcaa
     3661 tggtctcact cccgaaacca acaactactt ccgtaatgcg atcgctgaag acaatacgga
     3721 cagctacgac ggccgcgtgg actggacccc ctcttccaac gacacaatct ttggccgcta
     3781 caactactcc aaccgtttcc gctatatccc cggctatctg ggtgggctgg ccgatggcag
     3841 cagcacttcg gcctggggac ggcagaagct gaaaaaccac agctttgtcc tcggatggac
     3901 gcacatcttc agccccagcc tggtgaatga tttccgcttt ggatgggtgc gcaactacgc
     3961 ctttgcggaa caagacccct ttgccttaac gcaatatgca ggcaattttg tgcccggcat
     4021 tcctatcaat cctgcggtcg gcggaggtgt tccgcttacg accttctccg gctcgatcgg
     4081 agcgttcctc ggctcgcccg acttcctgcc caagcagcag gtgccgcagc agttccaata
     4141 taacgacacg ctttcactca cgcatggtac gcacaccttt aaagttggag cgtctcttta
     4201 tctgccgatg cgtaacctct tccaggacga gccaggaatg cgtggggact tgaccttcaa
     4261 tggacagttc acctgccagc gcaatgcatc aagccagtgc gtctccggaa caggcttccc
     4321 ttatgcagac ggtctgcttg ggctggtgca aagcaatcag cttacaaatg tgcactttgt
     4381 agaccagcgc ctgtggatgc tttcgggctt ctttgaagac gactggaagg tcacacccaa
     4441 agtcacgctg aatctgggac tgcgttatga atttgcaacg cctgcactca atgcaaaaga
     4501 ccagatggca aattttgatc ccatcgcagg tgcccttgtc tttgcgcgtt ccggcggact
     4561 gaagtcacgc gcgctggtca atccaaatta caagaacttt ggcccgcgca tcggttttgc
     4621 gtattcgccc gaccagaaga ccgtcctgcg cggtggatac ggcatttact ataccgtctt
     4681 tgaacggtac ggcagtgaag atcaactctc gctcaacccg ccgtacctca tcaacaagac
     4741 gcagtcggcg cagtccacat ctaccactcc ggtcatgatt gcgcagcagg gttttccgtc
     4801 gaactatctt gacccttcga cgattaacct cagtaatctg actgcattcc acatccgcgc
     4861 gatgaactcc aatgacccta ctccttacgt gcagcagtgg agcttcggca ttcaacgcgc
     4921 cattggaaac acatggacag cggaagtaga ttacgtcggc accaagtcaa cacatctgga
     4981 catcatccgc gactacaatc agccgctgat ttccggcaac actgtgcaga cgacaaagaa
     5041 cagcgcgggc caggtagtcc cggtcattcc ttactcgaac tttgggcaga ttgagtacac
     5101 ggatgcgatt ggttatggca attacaacgg cctgcaggca agtctgaaac gccgcttcca
     5161 gaacggactg agcatccaga ccgcctatac gtattcgcac agtctggacg acgttccgga
     5221 agagctggag agcaactccg gcgacgcgcc caacggacgc aacccttcga tgtggtatgg
     5281 caacagcgac ttcgacattc gcaaccgcgt ctcggtgagc tatgtgtatg agctgccctt
     5341 cggacatggc aagccaatgc tcaaccatgg cgcgttgtcc tggatcctcg gcgattttcg
     5401 cacctcgggt gtcta
//
LOCUS       DASRBV010000246         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_320412, whole genome shotgun sequence.
ACCESSION   DASRBV010000246 DASRBV010000000
VERSION     DASRBV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320412"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFA02_09115"
     CDS             <1..864
                     /locus_tag="VFA02_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU10053.1"
                     /translation="GMWGYVKGGMGMVSFYFCDAAREAGAVVATGVPVAAILPGEGVL
                     LEGGEHIGAPVVISNADPKRTLTMLGNAADPAWKAKVESVPIEGCTVKLNVLLSELPN
                     FKARPGTRGPHHFGQINAPLSKEEWKQGFAAARAGHLPEHLWCELYFQSAHDDSVAPK
                     GQHTMSVFAQYVPYRFENGTWDDRRDKVRALALNSLARFCTNIPDAVIAAQVLGPPDI
                     EAKVGLTGGHIFQGECLPPYMWSNRLSARTPMRGFYLCGACTHPGGSVIGINGRNAAM
                     AVLRDLNLLPV"
     gene            886..1596
                     /gene="ispD"
                     /locus_tag="VFA02_09120"
     CDS             886..1596
                     /gene="ispD"
                     /locus_tag="VFA02_09120"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZU10054.1"
                     /translation="MRVFIILPAAGLGTRMAAGQPKQFLELAGAPILIHSLRAFAQVP
                     QVAAIYVAVRASEQERVEAQVREHGFREKVRIVTGGDTRQESVANALYAIQCADDDIV
                     LVHDAVRPLIDAATISRTIEAVEKHDAAIVGLPAIDTIKQVERTADGAIITATIPREY
                     IVQAQTPQGFRCGLLKRAFAEALADGFTGTDEASIVERAGAQVFVVPGSSANIKITQP
                     GDLELAEFYLAHPAASRR"
     gene            complement(1640..>2206)
                     /locus_tag="VFA02_09125"
     CDS             complement(1640..>2206)
                     /locus_tag="VFA02_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10055.1"
                     /translation="AQQISSGAWKSSSKETTAAVPAQSVDFKPGGSWKLLDSTGFSVQ
                     YPGNWDATENAGNAATIAPAGGVVNTANGNVTIYGVIIDDYRPQQSTDLATATNQLIA
                     SVQQGNPGMKAVTGVQDVLVNKQPGKSMEFVNQGASAGGAAERDWLVTISRNDGSLSY
                     LVFVAPQKDFESLRGTYEQMLRTFRVKQ"
BASE COUNT          448 a          662 c          638 g          458 t
ORIGIN      
        1 ggcatgtggg gatacgtaaa gggcggaatg ggcatggtct cgttttactt ctgcgacgcc
       61 gcgcgcgagg ccggcgctgt cgttgccaca ggcgtcccgg tagctgcaat tctgcccggc
      121 gagggcgtac tgcttgaggg aggagaacac atcggcgcgc ccgttgtcat ttcgaatgcc
      181 gacccaaagc ggacactcac catgctgggc aacgccgccg accccgcatg gaaggcaaag
      241 gttgaatccg tgcccatcga gggctgcacc gtaaagctga atgtgctgct gagtgaactg
      301 ccgaatttca aagcacgccc gggcactcgc gggccccacc acttcgggca gatcaatgct
      361 ccgctcagca aagaagaatg gaagcagggc ttcgcggcag cgcgcgccgg acacttgccg
      421 gagcatctct ggtgcgagct ttattttcag agtgcgcacg acgacagcgt ggccccaaag
      481 ggccagcaca cgatgagcgt gtttgcgcag tatgtcccct acagatttga aaatggcacc
      541 tgggatgacc ggcgcgataa agtgcgcgct cttgcattga attcactggc gcgcttctgc
      601 acaaacattc cggacgcagt catcgccgcg caggtgctcg ggcctccaga tattgaagcg
      661 aaggtcggcc tcactggggg ccacatcttt cagggagaat gcctgccgcc ctatatgtgg
      721 tccaatcgcc tcagcgcgcg cactcccatg cgcgggtttt atctttgcgg agcatgcacg
      781 catcctggtg gaagcgtcat aggcataaac ggccgcaatg ctgccatggc cgtcctgcgc
      841 gacctgaatc tgcttcctgt atagtcatat ttctgggttg ttattatgcg ggtttttatc
      901 attcttccag cagcaggatt gggcacgcgc atggcagcgg gccagcctaa acaatttctc
      961 gaactggccg gcgcgccgat tctgattcac agccttcgcg ccttcgcgca ggtgccgcag
     1021 gtagcggcga tttacgtggc cgtgcgcgcc agtgagcagg aacgcgtgga agcacaagtg
     1081 cgggaacacg gcttcaggga aaaagtacgt attgtgactg gaggcgacac tcgccaggag
     1141 agcgtggcca atgccctcta tgcaatccag tgcgccgatg acgacatcgt gctggtgcat
     1201 gatgccgtac gtccactgat tgacgccgcc acgatttcgc gcaccattga ggcggtcgaa
     1261 aaacatgatg cagccatcgt ggggctgccc gcgattgaca ccattaaaca ggtcgagcgc
     1321 accgccgacg gggccatcat caccgctacg attccgcgcg aatatatcgt gcaggcgcag
     1381 acgccgcaag gcttccgttg tggactgttg aaacgggcct ttgccgaggc gttggccgac
     1441 ggatttaccg gcacggacga agccagcatc gtggaacgcg caggagcaca ggtcttcgtt
     1501 gttccgggct cgtctgcgaa cattaagatc actcaaccgg gcgatctgga gcttgccgaa
     1561 ttttatctcg cgcacccggc tgcatcgcgc cgataggtgg aaggcgctga ggattgcttc
     1621 gcaggttgag agcagagggt cattgcttca cgcgaaacgt cctcagcatc tgctcatagg
     1681 tcccgcgcag cgattcaaaa tccttctgcg gggccacgaa caccagatag ctcaacgacc
     1741 catcattgcg cgaaatcgtc accagccaat cgcgttccgc tgcacctccg gcagacgcgc
     1801 cctggttcac aaactccatg ctcttgcctg gctgtttgtt caccagaaca tcctgcacgc
     1861 cagtaaccgc cttcatgccc gggtttccct gctgcaccga agcaatcaac tggtttgttg
     1921 ccgttgccag gtcggttgat tgttgcggac ggtaatcgtc aataatcaca ccatagattg
     1981 tgacgttgcc attcgccgta ttcaccactc cgccggcagg agcaatcgta gctgcatttc
     2041 ccgcattctc cgtcgcatcc cagttgcctg gatactgcac agaaaatccg gttgaatcca
     2101 agagcttcca gcttccgcca ggtttaaagt ccacgctctg cgcgggaaca gccgccgtgg
     2161 tctccttgct gctgctcttc catgccccgc tggaaatctg ctgcgc
//
LOCUS       DASRBV010000247         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_165813, whole genome shotgun sequence.
ACCESSION   DASRBV010000247 DASRBV010000000
VERSION     DASRBV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165813"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..436)
                     /locus_tag="VFA02_09130"
     CDS             complement(77..436)
                     /locus_tag="VFA02_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU10056.1"
                     /translation="MNSLRYLGLVLFAGAVMFSGCSPSLPPSKPLDQLTPEEAQGRQV
                     FLGRCARCHRTDSSAGLNGPGLFGLYRKKYLRSGAPANDDRVSDIIEHGRGMMPAMGN
                     TMDEEQLQALLAYLHTL"
     gene            complement(440..2023)
                     /gene="dacB"
                     /locus_tag="VFA02_09135"
     CDS             complement(440..2023)
                     /gene="dacB"
                     /locus_tag="VFA02_09135"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZU10057.1"
                     /translation="MLTTARNSLIACLIVLCCTLPASAKSHRSKHKALAREIQNILKD
                     PMVARAHWGISVVRPDGTPVYALNDEQYFQPASNAKLFTTATALALLGPDFRMKTYVV
                     ADGPVDSDGHLRGSLRLIGGGDPSLSRRVYPYNGHSEHTQNPLQALDDLAAQVASSGI
                     KALDGTVVADDTLFVYERYGQGWGQDDLMWDYGAPVSALTINDNTRTLTLAPGIRAGD
                     KAVVTWNPALPDDALLLKNEAVTSPAGTQSHLGLDRQVDQSVLRAFGSIPQGSKPLDF
                     DIAVQDSAKFAGDAFVQALSAKGIQVNGAVQVSHRLSGDTQDFNAETHQPIALKPLTE
                     RQLPFTPPAGTRIVAQRTSPPLGQIVTVVNKVSQNLHAEMLLRMLGRTEGDDGSIAQG
                     ARVVRQFLTSAGVPPDGYFFFDGSGLSPQDVITPRAATTLLTYAARQPWGEVYRASLP
                     VGGVDGTLSNRFKNSPLKGKVFAKTGTLAEVHTLSGYLIAASGRTLVFSILCNDHSPV
                     TDSTREAIDKIAEAIAEAN"
     gene            2056..2958
                     /gene="folP"
                     /locus_tag="VFA02_09140"
     CDS             2056..2958
                     /gene="folP"
                     /locus_tag="VFA02_09140"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZU10058.1"
                     /translation="MTFTRRSKFTWELRTRSLEMGSRTLVMGVLNVTPDSFSDGGQFL
                     HVSTAVPQGLRMLDEGADILDIGGESTRPGQKEVLSAEEEQARVLPVIEGIRRARPDV
                     IISVDTYKAATAIAAVRAGAEIINDVSGLLWDAEMAAACARLECGVVLMHTRGRPDQW
                     KSQPPLKPEEVLPLVRNELRERLEDARKAGIAREKIVLDPGYGFGKRLDENYPLLANQ
                     SELLELDQPLLVGVSRKSFLAHALKPLYQGKDAPIAARLHATIAATTAAILAGASVVR
                     VHDVRPVVEAAHIADAILQGDVRH"
BASE COUNT          656 a          825 c          881 g          634 t
ORIGIN      
        1 agcccaatgc cgcagagaat gagcatccag agcgagatga cggccatgcc gggcagcggt
       61 ccagtgattt cttttgtcat agcgtatgca gataggcaag cagcgcctgc aactgttcct
      121 catccatggt attccccatg gcgggcatca tgccgcgtcc atgctcaatg atgtcgctca
      181 cgcggtcatc attggccgga gcgccactgc gcagatactt cttccggtaa agcccgaaca
      241 gtcccggccc gttcagcccg gcgctgctgt ccgtccggtg gcagcgcgcg cagcggccaa
      301 gaaagacctg acgcccctgc gcttcttccg gagtaagctg gtccagcggt ttggatggag
      361 gcagagacgg gctgcatccg gaaaacatga ccgctcctgc aaaaagcaca agaccaagat
      421 atctcaaaga attcaagcgt taattcgcct ccgcaatcgc ttcagcaatc ttatctatcg
      481 cctcgcgcgt gctgtcggtt acaggagagt ggtcattgca cagaatagag aagaccagag
      541 tgcgtccgct ggcagcaatg agatagccgc tgagtgtgtg gacttcggcc agcgtacctg
      601 tcttggcgaa gacttttcct ttgagtgggc tgtttttaaa gcgattggac aaggttccgt
      661 ccactcctcc aacaggcagc gatgcgcgat acacctcgcc ccacggctgc cgtgcagcat
      721 aagtaagaag agtcgtcgcg gcacgcggag tgatcacgtc ctgcggcgag aggccagagc
      781 cgtcgaaaaa gaagtagccg tctggaggaa caccggcgct cgtcaggaat tgcctcacta
      841 cgcgcgcgcc ctgtgcaatc gaaccatcat cgccttctgt cctgccaagc atcctcagca
      901 gcatctcggc atgaaggttc tggctgacct tgttcacaac cgtaacaatc tgtcccagcg
      961 gcggagaagt gcgctgcgcc acgatgcgcg tccccgcagg aggtgtaaac ggaagctgcc
     1021 tctctgtcag cggctttagc gcgatcggtt gatgcgtctc ggcattgaag tcctgcgtgt
     1081 ctccagagag gcggtggctc acctgtaccg cgccgttcac ctgaattccc tttgcggaca
     1141 gcgcctgaac aaacgcatcg cccgcgaact tcgccgaatc ctgtacggca atgtcgaaat
     1201 cgagtggctt gctgccttgt ggaatcgaac caaaggcgcg caggacgctc tgatcaacct
     1261 gacggtcaag acccaggtgg gactgggtcc ctgcgggaga ggtcacagcc tcgtttttca
     1321 gcagcagcgc atcatcaggt aatgcggggt tccatgtaac gacggcttta tcgccggcgc
     1381 gtatgccggg agcaagtgtg agtgtgcgcg tattatcgtt tatagtcagg gcagaaacag
     1441 gcgcgccgta gtcccacatc agatcatcct gtccccagcc ctgcccatag cgctcataga
     1501 caaagagtgt atcgtcggcg accacggttc catcgagcgc cttgatgcca ctggatgcca
     1561 cttgcgcagc cagatcgtcg agcgcctgca atggattctg cgtatgttca gaatggccgt
     1621 tatagggata cacacgcctg gagagagaag gatctccgcc gccaatcaag cgtagggagc
     1681 cgcgaaggtg cccatcggaa tccaccggcc catctgccac cacatatgtc ttcatgcgaa
     1741 aatcaggccc aagcaacgcc agtgctgttg ctgtcgtaaa cagtttggca ttcgatgcgg
     1801 gctggaaata ctgttcatcg ttaagtgcat agaccggggt gccgtcgggc ctaacgactg
     1861 aaattcccca atgcgcgcgt gccaccatgg ggtctttgag aatgttctgg atttccctgg
     1921 caagggcctt atgtttcgag cgatggcttt tggccgaagc gggcagcgta cagcaaagaa
     1981 caatcagaca ggcaatgaga gaattacggg cggttgtgag cattttgtcc ttgccgcgaa
     2041 gtgtagcatt ttcatatgac cttcacgcgg cgcagcaagt ttacgtggga attacgcacc
     2101 cgctctcttg aaatgggaag ccggacgctt gtgatggggg tcctcaatgt tacgccggat
     2161 tctttttctg atggcggcca gtttttgcat gtgagcacag ccgtaccaca aggcctgcgc
     2221 atgctcgatg aaggggcgga cattctcgac atcggtggtg agtccacccg ccctggccaa
     2281 aaagaagtac tctcagcaga ggaagagcag gcacgtgtcc ttccagtgat agaaggtatt
     2341 cgtagagcgc ggccagatgt cattatttcg gttgatacat acaaagctgc tactgcaatt
     2401 gccgcagtac gggcgggcgc agaaattatc aatgatgtga gcgggcttct gtgggacgca
     2461 gaaatggctg cggcctgcgc gagattggaa tgcggcgttg tcctgatgca tacacgcgga
     2521 agacctgatc agtggaaatc gcaacctcca ctgaagcccg aagaagtgct gcctctggtc
     2581 aggaatgagc tgcgtgagcg cctggaggat gcgcgaaagg ccggcattgc gcgtgaaaag
     2641 attgtccttg accccggata tggcttcggc aagcggctgg atgagaatta tccgttgttg
     2701 gccaaccaga gcgaattgct ggaactggac cagccgctcc ttgttggggt gtcgcggaaa
     2761 tcttttttag cccatgcgct caagccgttg tatcagggaa aagatgcacc aattgcagcc
     2821 cggctccatg ccaccatcgc ggcgacgacc gcagcaatcc ttgccggggc ctctgtggtt
     2881 cgcgtacatg atgtgcgtcc cgtggtggaa gcggcacata ttgccgatgc aattctgcag
     2941 ggtgatgtca gacattagcc tgcagtgctt gcgccagcag agcggtcaga gtttga
//
LOCUS       DASRBV010000248         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_320659, whole genome shotgun sequence.
ACCESSION   DASRBV010000248 DASRBV010000000
VERSION     DASRBV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320659"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..990
                     /locus_tag="VFA02_09145"
     CDS             52..990
                     /locus_tag="VFA02_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZU10059.1"
                     /translation="MQALYPFRLAPYFRTRIWGFHDLSPWFDYKTEGEPIGEVWLTGE
                     MCAADTGPLKGQPLKDITAAHGEALLGKGRAGEEFPLLIKVLFPKEKLSVQVHPDDAM
                     AQRYGEPRGKTECWYALDAKPGAEVALGIKPGTTPEQVRKAIEESRLEELLVWLPVHK
                     GDMIFVDAGTVHAIGPGAVLLETQQTSDLTYRMYDYGRPRELHLDKSMEAMRLTTGAG
                     KVLPKSVNAHSVLVDKKYFRVERWQQQLPSAENAYADAVQMLFAAEGNLTIEGKGFEP
                     FTLKRCQLAVVPAGAKDWTVKTQSAAEVMRILPQSA"
     gene            1084..2199
                     /locus_tag="VFA02_09150"
     CDS             1084..2199
                     /locus_tag="VFA02_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HZU10060.1"
                     /translation="MPKAIDFRPIILAGGSGTRFWPRSRKARAKQVLALDGERTMIQR
                     TVDRLLPLAKREDFWVITNDLLSPAICEQLDVVPEEHVVCEPAARNTAPAAGLAAFLV
                     EKQAPDAVLGVFPADHVIGDEAAFLSVLEHAARIAAAGENMVVLGVTPTRPETGYGYI
                     ETGEALDDGSLRVRRFTEKPNHEKAEEFVAAGNYHWNSGIFVWSARTLANAVREHLPE
                     TAPFLEEIAAAYGTPGFRKTFEELYPKCENISVDYAVLEPRSAKGEHHSNLYCLRANF
                     GWNDLGSWAALYEHQLAQAQCVDKHENVTETAGSLALDAHGNYVYSPKKFVALVGVKD
                     LVVVETDDAVLIASRDHSQDVGKIVKQLAADGKKELI"
     gene            2336..>3277
                     /locus_tag="VFA02_09155"
     CDS             2336..>3277
                     /locus_tag="VFA02_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZU10061.1"
                     /translation="MGNTNVIQFGTDGWRGVIADDFTFDNVRLAAAAIGHYILSKKDP
                     GEGVCIGFDTRFGSQAFAQTVAEVLASAGIHVKLADRITPTPALSYGVRAHCAAGGVM
                     ITSSHNPYQWNGVKYKAYYGGSGKPSIIAEIESYLGRPLPKTATPAKIETVDFQTPYV
                     EAISKFADLDLISKSGLRFAVDCMYGAGRGVLAGIFTQIGVDFVEIRAQHDPLFPGIN
                     PEPIEPHIRALQETTVQQKCHAGFATDGDADRIGAVDENGNFVDPHKIFAVILQWLLE
                     RKKWPGDVTRAFNTTKMLDRICARYGRTLHEHGIGFKY"
BASE COUNT          736 a          840 c         1016 g          685 t
ORIGIN      
        1 gatttttatc ggtgatgttt cccacgaaaa tttcaggcat actgaaagta catgcaagcc
       61 ttatatccat tcagacttgc tccttatttt cggacccgca tctgggggtt ccacgacctt
      121 tcaccgtggt ttgattacaa aactgaaggc gagccgattg gcgaagtctg gctgaccgga
      181 gagatgtgcg cggcagacac cgggccgctc aagggccagc cgctcaaaga cattactgcc
      241 gcacatggcg aggcgctgtt gggcaaagga cgcgcgggag aggagtttcc gctgctgatt
      301 aaagtgcttt tcccgaagga aaagctcagc gtacaggtgc atcctgacga tgcgatggcg
      361 cagcgctatg gcgagccgcg cggcaagact gaatgctggt acgcgctcga tgcaaagccg
      421 ggggccgaag tggcattggg catcaagccg ggtacaactc ctgagcaggt acgcaaggcg
      481 attgaggaat cgcgcctgga agaattgctg gtctggctac ctgttcataa aggcgacatg
      541 atttttgtgg atgctggaac tgtgcatgcc atcgggccgg gcgccgtgct tctggagacg
      601 cagcagacca gcgaccttac gtatcggatg tatgactacg ggcgtccgcg cgagctgcat
      661 ctggacaagt ccatggaggc gatgcgcctt acgaccggag cagggaaggt gctaccgaag
      721 agcgtgaatg cgcattccgt gctggtggac aagaaatatt tccgtgttga gcggtggcag
      781 cagcagcttc cgtcggcgga aaacgcctat gcggatgctg tccaaatgct ttttgcagcg
      841 gagggcaatc tgaccatcga aggaaaaggc ttcgaaccgt tcactctgaa acgttgtcaa
      901 ctggccgtcg tcccggcagg cgcaaaggac tggacagtca aaacacaaag cgccgctgaa
      961 gtcatgcgta ttcttccaca gtctgcctga catagcgagt ttttagtttc cagtcatcag
     1021 tgatgcgtca agacaagtgt cactgaagct ggcaaccggg aactgaaaac caataggaga
     1081 gccatgccaa aggcaattga ttttcggcca atcattctgg ctgggggaag cggtacgcgc
     1141 ttctggccac ggagccgcaa ggcgcgcgca aaacaggtgc tcgcgctgga tggcgaacgc
     1201 acgatgatcc agcggacagt ggaccgcctg cttccgctag ccaaacggga ggacttctgg
     1261 gtcattacca acgacctgct ctcgccggcc atttgcgagc aactggatgt tgttcccgag
     1321 gagcatgtgg tctgcgagcc tgcggcgcgt aataccgctc cggcagcagg tctggcggcg
     1381 tttctggtgg agaagcaggc ccctgatgcg gtgctggggg tctttcccgc ggaccacgtg
     1441 attggcgatg aggccgcgtt tctgtctgtg ctggagcacg ctgcccgcat tgctgctgcc
     1501 ggcgaaaaca tggtggtgct gggagtgacg ccgacacgtc ctgaaaccgg atacggctat
     1561 atcgaaacgg gcgaggcgct cgatgacgga tcgttgcgcg tgcgccggtt tacagaaaag
     1621 cccaaccatg agaaggcgga ggagtttgtc gccgcaggca attaccactg gaacagcggc
     1681 atctttgtat ggagtgcgcg gacactggcg aatgcggtgc gcgagcacct tccggagaca
     1741 gcgccgtttc ttgaggagat tgctgctgca tatggcacgc ccgggttccg gaagaccttt
     1801 gaagagcttt atccgaagtg cgagaacatc agtgtggatt acgcagtgct cgagccgcgt
     1861 tcggcaaagg gtgagcacca ttcgaatctt tattgtctgc gagcgaattt tggctggaac
     1921 gacctcggct cctgggcggc gctctatgag caccagcttg cgcaggcgca gtgcgtggac
     1981 aagcatgaga atgttacgga aaccgctgga agtctcgcgc tcgatgcgca cggaaattac
     2041 gtctacagtc cgaaaaagtt cgtagcgctg gtgggagtga aggacctggt ggtggtggag
     2101 accgacgatg cggttttgat tgccagccgc gaccactcgc aggacgtagg gaaaatcgtg
     2161 aagcagttgg cagcggacgg gaagaaggaa ctgatctgag agttgtggtt tccccatcct
     2221 ttcagccaaa gacggtccga aagggcaggg gaacctggca attccaggtt gtacatcagg
     2281 gacacccgaa cgtcactcag ggatgaaaaa tctggtttgg gattaagaaa aaagcatggg
     2341 aaatacaaac gtcattcagt ttggcactga cggctggcga ggagtcatcg ccgacgattt
     2401 cacctttgac aatgtgcgcc tggctgcggc ggccatcgga cattatattt tgtcgaaaaa
     2461 agaccccgga gaaggggttt gcatcggttt tgatacgcgt tttggctcgc aggcctttgc
     2521 ccagaccgtt gccgaggtgc tggcgtccgc gggcattcat gtaaagctgg ccgaccgcat
     2581 tacgccgact ccggccctat cttatggagt gcgcgcgcac tgcgctgcgg gcggcgtcat
     2641 gattacgtcg agccacaatc cttaccaatg gaatggtgtg aagtacaagg catattacgg
     2701 cggctcggga aagccttcga tcatcgcgga gattgaaagc tatctcggaa ggccgctgcc
     2761 aaagaccgcg actccggcaa agattgaaac agttgatttt cagacgccgt atgtcgaagc
     2821 catctctaaa tttgctgatc tggaccttat cagtaagtcg ggcctgaggt ttgccgtgga
     2881 ctgcatgtat ggcgcgggac ggggcgtgct ggcgggcatc tttacccaga tcggcgtgga
     2941 ctttgtggaa attcgtgcac agcacgatcc gctctttccc ggaatcaatc ctgagccgat
     3001 tgagccgcac attcgcgctc tgcaggaaac gaccgtccag cagaaatgcc atgcaggttt
     3061 tgccacggat ggtgacgcag accgcattgg cgcagtggac gagaatggca acttcgttga
     3121 cccccacaag atttttgccg tcattctgca atggctgctc gaacgcaaaa aatggcccgg
     3181 cgatgtgacc cgcgccttca acacaacgaa gatgctcgac cgcatctgcg cccgttatgg
     3241 gcgtacgctc catgaacacg ggattggatt taagtat
//
LOCUS       DASRBV010000249         8436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_526062, whole genome shotgun sequence.
ACCESSION   DASRBV010000249 DASRBV010000000
VERSION     DASRBV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526062"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VFA02_09160"
     CDS             complement(<1..1803)
                     /locus_tag="VFA02_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10062.1"
                     /translation="METLLQNLRYAARQLWKHPLFTLTAVLTLALGIGANTAIFTVVQ
                     SVLLAPLPYADAGRIVALGTLFTEKDRIHYRVTGPDAVDVRTQAHSLEAVSLYNEGLQ
                     GVQLRDHAVFTPVTIVDADFARVFGLVPIAGRNFTEQEIGRAALVSEQFARDQFGSVQ
                     AALGQTVSFENQPLQITGVLPASFDFPGKTQVWVTYPFHPEAENRTAYNYQAVAKLRA
                     GVSIDRARAELKTLSQHLAAIYPDSNQNKQMYVASLQQELTGKVRPMLMLLLAAVSMV
                     LLIACVNVAHLMLARSIDRQRELAVRTALGSSRWKLARLVLAESFLVSLMGACLGVLL
                     ALPVLRILLVMAPADLPRAAEIHLNGWVLAFTLTLSLLTTILSSLVPARRASKIDPVE
                     ALKADSSRGISAKSASALRNGLVMAEIAATFLLAVGAGLLLRTMMTLMDRDLGYQPGN
                     LLVVNAEAPGDDVKSGQAVARKYDDIFAQLKALPGVENAAGIYGLPTTNTGSNGGYSV
                     RGVPADYYHMPWADFSVVTPEYFTTMKIPLLRGRNFNVGDRFDSQLVAIISESVAKQS
                     FGDTDPIGKQIQLGLDLESLNKWITIIGVAGDVRQ"
     gene            1890..2366
                     /locus_tag="VFA02_09165"
     CDS             1890..2366
                     /locus_tag="VFA02_09165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10063.1"
                     /translation="MEIRFATAADLPAITRLINQAFSVELFFKAGDRITREEVEQLYE
                     KGHFMVLEEGGQIQGSVYVELRGERAYLGLLSVNPEKQRSGTGSRLTAAAEEFARESG
                     CRSMDLRVVNLREELPVIYRKFGYEVTGTEDVSVEAKQHFTKSAHFITMSKELGHR"
     gene            complement(2374..3510)
                     /gene="dnaJ"
                     /locus_tag="VFA02_09170"
     CDS             complement(2374..3510)
                     /gene="dnaJ"
                     /locus_tag="VFA02_09170"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZU10064.1"
                     /translation="MSSTANVTKADYYEILGVSRDASEQELKAAYRKLAMKYHPDRNP
                     GNKEAEEKFKECSEAYQVLTDPQKRAAYDRYGHAGVSGVGASNGDPFAGMPDLGDIFG
                     DFFGEVFNMGGGARRGSRAQRGRDVRVDHAIEFEDAVFGKEVQVTLRRMEACETCHGT
                     GTASGRGPSTCPQCQGRGQVRFQQGFFSIARTCSACGGTGTVISDPCRTCRGDGRVER
                     QRTIQVTIPAGVEEGTRIRYQGEGDAGRQGGPSGDLYIVLSVKPHAFFERDGNDLHCV
                     VPISFPQAALGDEITIPTLEGDTVLKIPEGTQSGKEFRIRGKGVPYLNEHGRGDLVVQ
                     VVVQTPKKLTKVQKELLRQLSESLAIENKPTSRSLFSKVKEMFS"
     gene            complement(3507..4085)
                     /locus_tag="VFA02_09175"
     CDS             complement(3507..4085)
                     /locus_tag="VFA02_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZU10065.1"
                     /translation="MSRKTVEEQQVNTEDQQQANGAENASAEPLVPVETSNVPQEEYD
                     RLKAERDMLVDRLARLQAEFENYRKREARERAEFRDFATASAVEQFLPVLDNFQLALK
                     STGSAEQLRTGVELIVRQMEEALRALNVQPVETVGMEFNPHVHEALETVERPDLPDHQ
                     VFEEVRRGYRMKERLLRPALVRVVSNPQQKEA"
     gene            complement(4152..5213)
                     /gene="hrcA"
                     /locus_tag="VFA02_09180"
     CDS             complement(4152..5213)
                     /gene="hrcA"
                     /locus_tag="VFA02_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZU10066.1"
                     /translation="MSSEVRISPRDRMVLTSVIEIYIGTGEPVASQAVARQFGEKEGM
                     SAATIRNVMASLDEAGLLEQPHTSAGRIPTARAFRFYVNQLNGAAPSAPLTPERREQI
                     DGSFAGITSSQQFLERTSHVLALISSGVGVALAPASDIHALEHIHFSRLTAGRVLAVV
                     VTKAGLVMDRVLALDHDLNQSELEVSARFLNENFHGWSIERIRDELARRMEQERNEYD
                     RLMRSVHELCRKGAFNTEGSGQTIFVEGVANLLASELDRERLQQMLRALEAKQRMVEL
                     LNAYVDARQQNVRVVVGLEEAVPEMQDLVLIGAPARLDGSNFGAVAVIGPTRIHYQET
                     MNAVSYIAQLSDRLFQPLQ"
     gene            complement(5376..5936)
                     /gene="efp"
                     /locus_tag="VFA02_09185"
     CDS             complement(5376..5936)
                     /gene="efp"
                     /locus_tag="VFA02_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZU10067.1"
                     /translation="MAIPATQMRPGMIIRHNNELHAVFSVEHRTPGNLRAFIQAKLRN
                     LRSGAMFEHRFRSADAIDRVVVDEVPMEFLYNDGDDYYFMNTENYEQTHLKRETLGDA
                     VEYLTPNLQITVSFFDGKAVGIELPQTVELTVVETEPGLKSATASSVTKPAKTETGLV
                     VQVPPFINEGEKIRVDTAEGAYLSRA"
     gene            complement(6035..6766)
                     /locus_tag="VFA02_09190"
     CDS             complement(6035..6766)
                     /locus_tag="VFA02_09190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZU10068.1"
                     /translation="MTRRRWIADTWDEGTASLTGQQVSHLIRVLRAQPGMEYDIVAGD
                     RVWHAVIAGITGDSVRFNLVTEVEADPALPITLLLSIFKFDRLEWAIEKATELGVERI
                     VPIIARRSEKHLVQSAEKRVERWRRIAREAAQQSRRSDVPEIENVLPLKTAARQAGDA
                     ARLLLAEQERSTTLRRAVEEVLGNSGDEMPVICMAVGPEGGWTAEEEALFDAEGWKSV
                     SLGPRILRAETAAMTAMAVVAALLE"
     gene            complement(7060..7770)
                     /locus_tag="VFA02_09195"
     CDS             complement(7060..7770)
                     /locus_tag="VFA02_09195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZU10069.1"
                     /translation="MQGKSYRPVAPWWHTALLLTPILLFSFLGGQRSSHRAIAQHHLF
                     EYAVTLAWEWVLAAVVLWGIRMRRVPLRQLLGVRRASRREWVQDLLLAAAFWIAASVV
                     LAAIGILLRLAHSSVPEKTLVQLAPENALELTLWILLSISAGICEEIVFRGYLLQQFS
                     SVRNQLWVGVLASSLLFGVAHGYEGVAGIVVITVYGAMFCALAIARGSLRPGMMAHAW
                     HDIFSGIAWMLLHHSRMM"
     gene            7826..>8436
                     /locus_tag="VFA02_09200"
     CDS             7826..>8436
                     /locus_tag="VFA02_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZU10070.1"
                     /translation="MEDVVIVSAVRTPVGKFQGTLSGFSATQLGAIAVREAVKRAKLE
                     PAQVDECIMGNVVSAGLGQNPARQAALFGGLPPSVGAMTINKVCGSGLKAVALAAQAI
                     QTGNSEIVVAGGMESMTNAPYLLPQARGGFRMGNATVVDSMVHDGLWDVYNNYHMGQT
                     AENVAEKYKITREEQDEYALNSHRKAAAAWKECRFASQVVPVE"
BASE COUNT         1838 a         2376 c         2442 g         1780 t
ORIGIN      
        1 ctgccgcaca tcgcctgcta cgccaataat cgtgatccac ttgtttaggc tctcaaggtc
       61 aagaccaagc tggatctgct tgccgatagg atcggtatca ccaaagctct gcttcgcaac
      121 agactcactg atgattgcaa ccaactgact atcaaacctg tctccgacgt tgaagttgcg
      181 cccgcgaagc aatggaatct tcattgtggt gaagtattca ggagtgacaa cactgaagtc
      241 agcccacggc atgtggtagt aatcggcagg aacaccccgg acgctgtatc ctccgttgga
      301 tccggtgttt gttgtcggaa gcccatagat tccggcggcg ttttccacac cgggaagagc
      361 tttcaactga gcaaagatat cgtcgtactt gcgcgcaacg gcctgtccgg atttcacgtc
      421 gtctccaggc gcttcagcgt ttaccactaa cagatttcct ggttgatagc ccaggtcgcg
      481 gtccatgaga gtcatcatgg tgcgaagcag aagtcctgcg ccaacagcga gaagaaaagt
      541 ggcggcaatt tccgccatca ccagaccatt acgaagagca gaagcacttt tagctgaaat
      601 tccgcgcgaa ctatctgcct tcagcgcctc gacaggatca attttcgagg ccctccgggc
      661 cgggacaaga gaagacagga tggtggtcag cagggagagc gtcagcgtaa acgccagtac
      721 ccatccattc aggtggattt ccgcggcgcg gggcagatcg gcgggggcca ttaccagcaa
      781 tatgcggagg accggaagag caagcaatac gcccagacaa gcgcccatca gcgaaaccag
      841 aaaactctct gccagaacaa gccgggccag cttccagcgt gaagaaccca gcgctgtgcg
      901 gaccgcaagt tcgcgctgcc tgtcaatgga acgcgccagc atcagatgcg caacattgac
      961 gcaggcgatg agcagaacca tactgaccgc ggccaacaac agcatgagca tggggcggac
     1021 cttgcccgta agctcctgct gtaaggatgc gacatacatc tgcttgttct ggttggagtc
     1081 aggatagata gcagccagat gttgcgacag cgtcttcagc tcagcgcgcg ctctatctat
     1141 agataccccg gcccgcagtt tggccactgc ctgatagtta tatgcggtgc ggttttctgc
     1201 ctccggatga aaggggtaag tgacccagac ctgtgtttta ccaggaaaat caaaggaggc
     1261 gggcagaact ccggtaattt gcagcggctg gttttcaaaa ctcacggtct ggcccaacgc
     1321 agcctggaca cttccaaact ggtcacgagc aaattgttcg cttaccaacg ccgcgcggcc
     1381 gatttcctgc tctgtgaagt tgcggccagc gatgggaacc agcccgaaga cacgcgcaaa
     1441 atccgcgtca acgattgtga ccggagtgaa cacggcatgg tcgcgcaatt gcacgccctg
     1501 cagtccttcg ttatacaggc tgacggcttc caggctgtgc gcctgtgtgc ggacgtccac
     1561 cgcatcaggt ccagtaacac ggtagtggat tcgatctttc tctgtaaaga gcgtgcccag
     1621 cgccacaatc ctgccggcat cagcgtaagg cagcggggcg agtaaaaccg actggacgac
     1681 agtaaagatg gccgtgttcg cgccaatccc cagcgccagc gtcaggaccg cggtaagagt
     1741 aaatagcgga tgtttccata actgccgcgc cgcatagcgg agattctgta gaagagtttc
     1801 catggaccac ctgccgtttg cacaatgata gacaaatacg gaagtcgtgc aacgtctgtt
     1861 ccgtctatca tgtttgcgat actggtttca tggaaatccg ctttgctact gctgctgacc
     1921 tgcccgccat tacgagactt atcaatcagg cattttctgt agagcttttc tttaaagccg
     1981 gagaccgcat tacacgggaa gaagtcgagc agctttatga gaaaggccat tttatggtgc
     2041 tcgaagaagg gggccagata cagggcagcg tctatgttga gctacgtggc gagcgcgctt
     2101 atctgggttt gttgtccgtc aaccctgaaa agcagcgctc ggggaccggc tcgcgcctga
     2161 cggcggccgc agaagagttc gcacgcgaat caggctgccg ctccatggac ctgcgggtcg
     2221 tgaatctgcg cgaagagctg cccgtcatct acaggaaatt cggatacgag gtgacgggga
     2281 cggaagatgt ctcagtggag gcaaagcagc atttcacgaa gtctgctcac tttatcacga
     2341 tgtcaaaaga acttggccat cgctgagaag aaatcaactg aacatctcct ttactttgga
     2401 gaacaggctt cgtgatgtgg gcttgttttc gatggccaga gactcggaaa gctggcgcag
     2461 gagttctttc tgtactttcg tcagcttctt tggtgtctgg accacaacct gcacgacgag
     2521 gtcgccgcgt ccatgctcat tgaggtaagg aacgcccttg ccgcggatac ggaactcctt
     2581 gccgctctgc gtgccttcgg gaattttgag cacggtatcg ccttcaagcg tgggaatcgt
     2641 gatttcgtct ccgagggccg cctgcggaaa cgagatgggg acaacgcagt gcaggtcatt
     2701 tccgtcgcgt tcaaagaacg catgtggttt gacggaaaga acgatgtaga gatcgccgga
     2761 tggccctccc tgacgcccgg catcgccctc gccctgatag cggatgcgcg tgccttcctc
     2821 gacaccggca ggaatggtga cctggatggt gcgctggcgt tcgacacggc catcgccccg
     2881 gcaggtgcgg catgggtcgg agatgacggt acctgtacct ccgcaggccg agcaagtgcg
     2941 ggcaattgag aagaagccct gctggaagcg gacttgtccg cgcccctggc actgcgggca
     3001 ggtggagggg ccacgtccgc tggccgtacc tgtaccgtgg caggtttcac aggcttccat
     3061 gcggcgaagg gtaacctgca cctctttgcc aaagacggca tcctcaaatt caatcgcgtg
     3121 gtccacgcgc acatcgcgtc cacgctgggc gcgggaaccg cggcgggcgc ctccacccat
     3181 gttgaagact tcgccgaaga aatcgccgaa gatgtcgccg aggtcgggca ttccggcaaa
     3241 cggatcgccg ttcgaggcgc ctacgccact gacaccggca tgtccataac ggtcataggc
     3301 ggcgcgcttc tgtggatcgg tgaggacctg gtacgcctcg ctgcattcct tgaacttctc
     3361 ctccgcttcc ttatttccag gattgcggtc aggatgatat ttcatggcga gcttgcggta
     3421 ggcggccttc agctcctgct cggaggcgtc gcgcgagaca cccagaattt cgtaataatc
     3481 ggctttcgtc acgttagctg tactactcat gcttctttct gctgcggatt actgaccacg
     3541 cggaccaggg caggccgcag cagccgttcc ttcatgcgat atccgcggcg gacttcctca
     3601 aacacctgat ggtccggcaa gtccggtctt tcgaccgtct ccagggcctc atgcacgtgc
     3661 ggattgaatt ccataccgac ggtctccacg ggctgaacat tcaacgcgcg cagcgcctct
     3721 tccatctgcc tgacgatcag ctcgaccccg gtgcggagct gctcggcaga gcccgtggac
     3781 ttgagcgcaa gctggaaatt gtccagcaca gggagaaatt gctcgaccgc gctggccgtg
     3841 gcaaagtcgc ggaactcggc gcgctcgcgg gcttcgcgct tgcgatagtt ttcaaattcg
     3901 gcctggagac gtgcgagtcg gtctaccagc atgtcgcgct cagccttcag gcggtcatat
     3961 tcttcctgcg gaacattgga tgtctcaacc gggacgaggg gttcagcgga tgcgttttcc
     4021 gccccgttgg cctgttgttg gtcttcggtg tttacctgtt gttcttccac tgtttttctg
     4081 ctcatacttc aaaccttgtc cggacgcgtt tgtgcgcccc agttatagat caccctgcgt
     4141 atgttcagat gctattgcag aggctggaac agcctgtctg aaagctgcgc aatgtaggaa
     4201 acggcattca tcgtctcctg atagtggata cgtgtgggac caatgacagc aacagcacca
     4261 aaattgcttc catccagccg tgcgggggcg ccaatgagca ccaggtcctg catttcgggg
     4321 acggcttctt ccagtccgac gacgacgcgt acgttctgct ggcgcgcgtc tacatacgca
     4381 ttcagtaatt ccaccatccg ctgcttggct tccagagcgc gcagcatctg ttgcagccgc
     4441 tcgcggtcga gctcactggc cagcagattg gcgacgcctt ccacaaagat ggtctgcccg
     4501 ctgccctccg tattgaatgc gcctttgcgg caaagctcgt gaacagagcg catgagacgg
     4561 tcgtattcgt tgcgctcctg ttccatgcgg cgggccagtt cgtcgcggat ccgctcaatc
     4621 gaccagccgt ggaaattctc attcaggaac cgcgccgaga cttccagctc gctctgattt
     4681 aaatcatgat caagagcaag gacgcggtcc atgaccagtc cggccttagt gacgacgaca
     4741 gcgaggacgc gtccggcggt aaggcggctg aagtgaatat gttcgagcgc atggatgtca
     4801 gaagcgggag ccagcgcaac tcccacgcca ctggaaatca gggccagaac atgcgatgtg
     4861 cgctccagaa attgctggct gctggtaatt ccggcaaagc tgccgtctat ctgctcgcgg
     4921 cgctccgggg taagaggagc ggaaggggcg gcaccgttca gctggttgac ataaaaacga
     4981 aacgcccggg ccgtggggat gcgtccggca gaagtatgcg gctgctcaag aagccctgct
     5041 tcatccagag aggccatcac attgcggatg gtcgcggcgc tcatgccttc tttttcgcca
     5101 aattgacgcg caacggcctg cgatgccacc ggctctccgg taccgatgta gatttcaatc
     5161 acagatgtca gcaccattcg gtcacgcgga ctgatgcgca cttcggaaga catggcttcg
     5221 tgcaccacct aataggtggt ctcctccctt tttattttaa agcatggtgg gaagacggcc
     5281 ggtattccgg attcatggag cgcatccagg ccataaaaca aaaacgatac ggcctgtggc
     5341 cgtatcgctg gtaacgctgg actttagaaa aaatcttatg cccgggacaa gtacgcacct
     5401 tcggcggtgt ccacgcggat cttctcacct tcattaataa acggaggcac ctgcacgacg
     5461 agaccagtct cagttttggc gggcttggtc acggacgatg ccgtggccga cttcagtccg
     5521 ggctccgtct cgacgacggt aagttctacg gtctgaggaa gttcgatgcc gacggccttg
     5581 ccatcaaaga atgagaccgt aatctgaagg ttcggcgtca gatattcgac agcatcgccc
     5641 agggtttcgc gcttcagatg ggtctgctcg taattttcgg tgttcatgaa gtagtaatca
     5701 tcgccgtcgt tataaagaaa ctccatcgga acttcatcta caacaacccg atcaatggcg
     5761 tcagccgagc ggaaacggtg ttcaaacatg gccccactgc gcaggttgcg gagcttggcc
     5821 tgaataaagg cgcgcagatt gcctggggtg cggtgctcga cggaaaagac cgcgtgcagt
     5881 tcattattgt gccggatgat catacccgga cgcatttggg tggcgggaat cgccataaaa
     5941 gaaactcctt actgttgttc tgggatttta cggatgcggg cgcacagggc ctcgcgggaa
     6001 actctctcca aagaatgatt gtaccttata ggcgttattc caataacgca gccactacgg
     6061 ccatcgccgt catggcagcc gtttcggcgc gcaggatacg cgggcccagg ctcacggact
     6121 tccatccttc tgcatcaaac aatgcctctt cttctgccgt ccatccgcct tcagggccga
     6181 cggccatgca gataacaggc atctcgtctc cggagtttcc gagtacctct tcaacggcgc
     6241 gccgcagggt cgttgagcgc tcctgctcgg cgagcagcaa ccgggcagca tcgccagcct
     6301 gacgcgcagc agttttgagc ggcagcacat tttcaatttc cggaacatcg gagcggcgcg
     6361 actgctgcgc agcctcacgg gcgatacgcc gccagcgctc gacacgtttt tcggccgact
     6421 gcaccagatg cttttcgctg cgccgggcaa tgatggggac gatgcgctcg acgccaagtt
     6481 cggtcgcttt ttcgatggcc cactccaggc ggtcaaactt aaagatggaa agcagcagcg
     6541 tgattggcag cgcgggatcg gcttctactt cagtgacaag attgaagcgg actgagtcgc
     6601 cggtgattcc ggcaatgact gcgtgccaga cgcggtcgcc tgcgacgatg tcgtattcca
     6661 ttccgggctg tgcgcgcaaa acgcggatga ggtgtgagac ctgctggcct gtgagcgatg
     6721 cagtgccttc gtcccatgtg tcggcgatcc agcgacgtcg tgtcattgca gtttctattg
     6781 tcacagaaac actaaggggg acgctggatg cgtccccctt atgtgttgcc tggaacgaag
     6841 ttacttcttc tttgaggttt tcttagctgc cttcttcgtt gccttcttgg ctgccttttt
     6901 cgttgccata gtttctattc tccctattcg tgtactgcgg aactacttcg ccgcagttaa
     6961 cgaatgtata gagtgctgag aaacaagtgt caagaaaaaa atgcacgcaa aaatcaattt
     7021 gttcgacgcg cgagaacgca gcaccacgcg cgcaggaact tacatcatgc gcgaatggtg
     7081 aagaagcatc catgcgatgc cggaaaaaat gtcgtgccat gcatgcgcca tcattcccgg
     7141 acggaggctt ccacgagcga tggcaagagc gcagaacatc gctccatata cagtgatgac
     7201 gacgattccc gccactcctt catatccatg cgcaacgcca aacagaagcg atgaggcaag
     7261 cacgcccacc cagagctggt tacgcacgct cgaaaactgc tgcaagagat atccgcgaaa
     7321 gacgatctct tcgcagattc cggccgagat gctgaggaga atccaaagtg ttaattcgag
     7381 ggcgttctcc ggagcaagct gcaccagcgt tttctctgga acagaagagt gcgcaaggcg
     7441 cagaaggatt ccaatggctg caagcaccac cgaagctgcg atccagaaag cggcggcgag
     7501 gagcaggtcc tgtacccatt ctctccgtga agcgcgccgg acgccgagca gttgacgcag
     7561 cggcacacgg cgcatccgta tgccccagag aacgacagcg gcgagcaccc attcccacgc
     7621 cagtgtgact gcgtactcaa aaagatggtg ctgtgcgatg gcgcgatggc tggagcgctg
     7681 tccgccaaga aaagagaaga gcagaatggg cgtaagcagt agcgcggtgt gccaccacgg
     7741 cgcgacggga cggtatgact ttccctgcat ggtttgattc tgctatcttc attcgttcgc
     7801 cgtctaataa ggagtgaggg ccgtcatgga agatgtagtg attgtttccg ccgtgcgcac
     7861 gccagttgga aaatttcagg gcacactgtc cggcttttcc gccacgcagc taggcgccat
     7921 cgcggtgcgt gaagcagtga agcgcgcgaa actcgagccc gcacaggtgg acgagtgcat
     7981 catgggcaat gtggtctctg ccggactggg gcagaacccg gcacgccagg ccgcgctctt
     8041 cggaggactt ccgccctcag ttggagcgat gactatcaat aaagtctgcg gctctggatt
     8101 gaaagcagta gctctggcgg cgcaggccat ccagaccggg aactcagaga tcgtcgttgc
     8161 cggcggaatg gagtccatga cgaacgcgcc gtatctgctg ccgcaggcgc gaggcggctt
     8221 ccgtatgggc aacgcaactg tggtggactc gatggtccat gacggcctgt gggacgtcta
     8281 caacaactac cacatgggcc agactgcgga gaacgttgcg gagaaatata aaatcacgcg
     8341 cgaggaacag gatgagtatg cgctgaactc ccaccgcaaa gccgcggcag catggaagga
     8401 atgccgcttt gcttcgcagg tggtcccggt ggagat
//
LOCUS       DASRBV010000250         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_526239, whole genome shotgun sequence.
ACCESSION   DASRBV010000250 DASRBV010000000
VERSION     DASRBV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526239"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="pdxA"
                     /locus_tag="VFA02_09205"
     CDS             <1..971
                     /gene="pdxA"
                     /locus_tag="VFA02_09205"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZU10071.1"
                     /translation="AVTMGDSSGVGPEILLKAFHQKEIRYPFVVYGDLEVLEFYDDLL
                     KYGVRFRKVSVAKDYQPGYVNVIDAGIMRRDDVTVGQLNRKSGLAAREYVVAATRAAL
                     AGETAAIVTLPINKEATQLSDPGFTGHTELIGELCGAKDVTIMLASDELIVTHVSTHV
                     SLSKAIARVKKERIETIIRLTHEAVRKLRGSPRIAVAGLNPHAGENGLFGDEEIREIR
                     PAVEWARSQGWRVEGPLPPDTVFYFAVRKKAYDAVVCMYHDQGHIPSKLLDFEGGVNI
                     TLGLPIIRTSVDHGTAFDIAGQGKASTVSFIRAFDFAARLSSGGAR"
     gene            complement(1169..1621)
                     /locus_tag="VFA02_09210"
     CDS             complement(1169..1621)
                     /locus_tag="VFA02_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZU10072.1"
                     /translation="MIAAIVLAVLSAVIHALGLLGLLYWQTRQWPGIEADFRPRRNLP
                     VLLFIFTAILGLHIAEMLLWACFYSWQGALPRFEAAFYYSACSYTTVGYGDVVLGHPW
                     RLAGVTESLTGVLLLGWSAAFFFSVVSRLFEIRSHLWQQRRPSRTEEV"
     gene            complement(1627..>2194)
                     /locus_tag="VFA02_09215"
     CDS             complement(1627..>2194)
                     /locus_tag="VFA02_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HZU10073.1"
                     /translation="QARRLVHVGWALMALGMWYSTRSMDTLISFHVASWVRVLQVIGL
                     PLLFVPLTTVVYVGLPSEKNNAISALINFMRNIGMSFGTSIVTTILARRAQFHQQVLV
                     SHLTPGNVYASEALHKLTQALFHAGLSASDALNQAYGRIYHALIIQSVSLAYIDAYHV
                     FALAAAILFLLSFFLKKNEPRAPAGPTE"
BASE COUNT          502 a          589 c          680 g          423 t
ORIGIN      
        1 ttgccgtgac aatgggggat tcgagcggtg tcggaccgga gattcttctg aaggcttttc
       61 atcagaagga aatccgatat ccgttcgtgg tctatggaga cctggaagtg ctggagtttt
      121 atgacgacct cctgaagtac ggtgtccgat tccggaaggt gagtgttgca aaggattatc
      181 agccgggata tgtgaacgtc atcgatgccg gaatcatgcg gcgagacgac gttacggtgg
      241 gtcagttgaa caggaagtca ggcctggcgg cccgggaata cgttgtggcc gccacccgcg
      301 ccgcacttgc gggtgaaacc gctgccatcg ttaccttgcc gataaacaag gaagcgaccc
      361 agctttccga tcccggattc accggacata ccgaactgat cggcgaactc tgcggggcga
      421 aggacgtgac gatcatgctg gcatctgatg aactgattgt cacgcacgtc agcacacacg
      481 tttccttatc caaggccatc gcccgcgtaa aaaaagagcg catcgaaacg atcattcgtc
      541 tgacccatga agccgttcga aagctgagag gcagtccgcg cattgccgtg gccggcctga
      601 atcctcacgc aggcgagaac ggactgttcg gcgacgaaga gattcgcgag atcaggccgg
      661 ccgttgagtg ggcgcgcagc cagggctggc gtgtggaagg gccgctgcct cccgacacgg
      721 ttttctattt cgccgttcgc aagaaggcat acgacgcggt tgtctgcatg taccatgacc
      781 agggtcatat cccgagcaag ctgctggatt tcgagggcgg agtcaacatc acgctcggct
      841 tgccgattat ccgaacgtct gtcgatcatg ggacggcttt cgatatcgcc ggccagggaa
      901 aagcttcaac ggtcagcttt attcgagcct tcgatttcgc cgctcgcctg agttcgggcg
      961 gtgcgcgatg atacagacgt gcggcctcat tttcggccgg agcgccgttt tggcaaaaat
     1021 gcgactttaa gcaaaggccg gcttgcgcgc gatccatcgt ctatttcgtt caccgaaggg
     1081 gccagaccgt gctcatgccg gccgggaaaa gattaccctg cgctgaagtg aaacctgttg
     1141 cgccggtgga tggaatggaa gcgcttcctc agacctcttc agtgcgcgat ggcctgcgct
     1201 gttgccagag atggcttcga atttcgaaca agcggctgac aacactgaag aagaaggctg
     1261 ccgaccagcc gagcagcagc acgcccgtca gcgattccgt cacgcccgcc aatcgccatg
     1321 ggtgcccaag cacaacgtct ccgtaaccga ctgtcgtata gctgcacgca gagtagtaaa
     1381 aagcggcttc gaagcgtggc agtgcaccct gccaggagta aaagcaagcc cacagcaaca
     1441 tttcggcaat gtgcaggccc agaatggctg taaatatgaa caagagcacg gggagattgc
     1501 gtcggggacg gaagtccgct tcgattccag gccattgccg cgtttgccag tagagcagcc
     1561 ccaggagtcc caaggcatga atgaccgcgc tcaaaacggc caggacgatt gcggcaatca
     1621 tggtttctac tccgtgggtc cggcgggcgc gcgcggttcg ttctttttca ggaagaaaga
     1681 gagcaggaac agaattgcgg cggcgagggc aaagacgtgg taagcatcga tataggccag
     1741 ggagactgac tggatgatca gcgcgtggta tatgcggccg taagcctggt tgagcgcatc
     1801 ggaagcgctg agcccggcgt ggaagagcgc ctgcgttaac ttgtggaggg cctcggaggc
     1861 atacacgttg cccggcgtga gatgcgagac aagcacctgc tggtggaact gagcgcggcg
     1921 tgcgaggatc gtagtaacga ttgaagtccc gaagctcatt ccaatgttgc gcatgaagtt
     1981 gatgagggcg gagattgcgt tgttcttctc agacgggaga ccgacgtaaa ccactgttgt
     2041 aagcggaaca aacagcagag gcaggcctat gacctgaagc acgcgcaccc aggaagccac
     2101 gtggaagctg atcaaggtgt ccatgctccg cgttgaatac cacatgccca gggccattaa
     2161 cgcccagcca acatgcacaa gccgccgcgc ctga
//
LOCUS       DASRBV010000251         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_526244, whole genome shotgun sequence.
ACCESSION   DASRBV010000251 DASRBV010000000
VERSION     DASRBV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526244"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFA02_09220"
     CDS             complement(<1..318)
                     /locus_tag="VFA02_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HZU10074.1"
                     /translation="MQNVSVIKELHQERFQTAPELFIAPGRVNLIGEHTDYADGFVMP
                     AAIDFATLAAISARRDKQAVIYSENYGEEVSRHFDAIGTRGQHHWSDYPLGVLATLQQ
                     EGIP"
     gene            complement(364..1302)
                     /locus_tag="VFA02_09225"
     CDS             complement(364..1302)
                     /locus_tag="VFA02_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10075.1"
                     /translation="MEQAEQSGGNLVLENNHLRITIAPRLGGKIASIQLLPEHEELLQ
                     KPLQPYAPRRRYMPFDASDASGWDECLPSVAACEVETPSGKARVPDHGDFWQVEWQIV
                     SQNENGVTISADGFSLPLRFTKAITLAENQLKIAYKVENPANFPVEYIWSAHPLFAVD
                     PGDRIILPHSVKEVVVEGSAGNRLGPKGAKHAWPQTTLGHGPICDLSIAGRPEDGIGD
                     KLFTATPPGGWCAIERPKLGRRIQLQFHPHSTMYLGLWICYGAWPEGHANRQYCVALE
                     PCTATGDSLAEAQKAGRANRLAPGEKCEWVVVLQVV"
     gene            complement(1269..2594)
                     /locus_tag="VFA02_09230"
     CDS             complement(1269..2594)
                     /locus_tag="VFA02_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU10076.1"
                     /translation="MKQDPALELLNEFLPELQKSFTHLPAAGLEVAPLDAMRSVLAQT
                     ASRMADNYPYFHPLYAGQMLKPPHPIARAAYALAMWINPNNHALDGGRASSRLEIEAV
                     REIAAMFGWTKFLGHLTSGGTFANLEALWVAGQLAPGKKIAASEQAHYTHDRISAVLG
                     LPFVGVPADEHGRMDIAALETLLQSGEIGTVVVTLGTTSMGAVDPLPRVLALREKYHF
                     RVHVDAAYGGYFRLASNLAPEVAQAFARVHEADSIVVDPHKHGLQPYGCGCVLFRDPA
                     VGRFYKHDSPYTYFTSSELHLGEISLECSRAGAAAVALWATQKLLPLVPEGEFAAKLS
                     RSRQAALRVYQKIANDNRFLPGPEPELDILVWAVKADTLEESSRRAKEVFDRAARQDL
                     HLALAQLPAKCFGAQTGGKVTCLRSVLMKPEHLEWADEIWNRLSKAAEI"
     gene            complement(2609..>2893)
                     /locus_tag="VFA02_09235"
     CDS             complement(2609..>2893)
                     /locus_tag="VFA02_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZU10077.1"
                     /translation="CSHLGGKSMASACTHVKDHVRHVKPSSKGCEECLRTGDRWVHLR
                     MCLECGHVGCCDSSKNRHARAHFHATQHPIIQSAEPGEDWRWCYVDEAYL"
BASE COUNT          575 a          816 c          877 g          625 t
ORIGIN      
        1 gggaatgcct tcctgttgca gagtggccag tacgcccaga ggataatcgc tccagtgatg
       61 ttggccgcgc gtgccgattg catcaaaatg gcgcgagact tcttctccgt aattttctga
      121 gtaaatgacg gcctgtttgt ctctgcgcgc ggagatggct gcgagggtgg cgaagtcaat
      181 ggcggcaggc atcacgaagc catcggcata atcagtgtgt tcaccgatga gattgactct
      241 gcctggagcg atgaagagtt cgggagcggt ctggaaccgt tcctgatgaa gttctttgat
      301 aacgcttaca ttttgcatgc aaccatccct cagcaaaaat cgaacagtta gctttactgt
      361 acactaaacc acttgcagaa caaccaccca ctcgcatttc tctccgggag cgaggcggtt
      421 ggcgcggcct gctttctgcg cttcggcaag cgaatctcct gtcgctgtgc atggttcaag
      481 cgcgacgcag tattggcggt tggcatggcc ctcgggccat gctccgtagc agatccacag
      541 ccccagatac atggtcgaat gaggatggaa ttgaagctga atgcgacggc ccagcttagg
      601 gcgctcgatg gcgcaccatc cgcccggcgg cgtggccgtg aagagcttgt ctccgatgcc
      661 gtcttccggc ctgcccgcga tgctcaaatc gcagatgggg ccgtggccca gcgtagtttg
      721 cggccatgca tgcttcgccc ctttgggtcc gaggcggttt ccggccgagc cttccaccac
      781 gacttccttc acggaatgag gaagtatgat gcggtcgcct gggtctacgg caaagagcgg
      841 atgtgccgac cagatgtatt ccaccgggaa atttgccgga ttttcgactt tataggcgat
      901 tttcaactga ttttctgcca acgtaattgc tttggtgaag cgtaacggaa ggctgaagcc
      961 atccgcggag atggtgacac cgttttcatt ttgggagaca atctgccact ccacctgcca
     1021 gaaatctccg tgatcgggga cgcgggcctt tcctgaaggc gtttccacct cgcaggcggc
     1081 aacggagggc aggcattcgt cccatccgct ggcgtcgctt gcgtcaaagg gcatgtagcg
     1141 tctgcgcggg gcatagggct ggagcggttt ttgcagcagc tcttcatgtt ccggcaaaag
     1201 ctgtatggaa gcaatttttc cgccgaggcg aggtgcaatc gtgatgcgca ggtggttgtt
     1261 ttcaaggact agatttccgc cgctttgctc agcctgttcc atatttcgtc agcccactcc
     1321 agatgttccg gtttcatcag cacagagcgc aggcatgtta ctttgccacc ggtctgcgcg
     1381 ccaaagcact ttgcgggaag ctgcgcgagc gccagatgca ggtcctgcct tgctgcccgg
     1441 tcaaagacct ctttggcgcg gcgcgaagat tcttccaatg tatccgcttt cactgcccat
     1501 accagaatat ccagttccgg ctccggtccg ggaagaaacc ggttgtcatt tgctattttc
     1561 tgatagacgc gcagggcggc ctgccggctt cgggaaagct tcgcggcaaa ttcgccctcc
     1621 ggtacaagag gaagcaattt ctgtgtcgcc cagagcgcca cggcagcagc cccggcgcgc
     1681 gagcactcca ggctgatctc ccccagatgc agttcactcg aagtgaagta ggtgtaagga
     1741 gaatcgtgct tgtagaagcg gcccactgca gggtcgcgga agagcacaca gccgcatccg
     1801 tagggctgaa gtccatgttt gtgcgggtct acaacaatcg aatcggcttc atggacccgg
     1861 gcgaaggcct gagctacctc tggcgcgagg tttgaggcca gccggaagta gccgccatag
     1921 gccgcatcca catgcacgcg gaagtggtat ttctcgcgca atgcgagtac ccgcggcagc
     1981 ggatctacgg cgcccatcga agtggtcccc agagtgacca caacagttcc gatttcaccg
     2041 gactggagca gggtttcaag ggcagcaatg tccatgcgtc catgctcatc ggctggaacg
     2101 ccaacaaatg gcagcccgag cacggcgctg atccggtcat gggtgtaatg cgcctgttcg
     2161 gatgcggcga tcttctttcc cggagcaagc tgcccggcca cccataatgc ctcaagattg
     2221 gcgaatgtgc cgccgctggt caggtggccc agaaacttcg tccagccaaa catggcagcg
     2281 atttcgcgca ccgcttcgat ttccaggcgc gagctggcgc ggcctccatc gagtgcgtgg
     2341 ttattcggat tgatccacat ggccagggca taggcagcgc gcgcgatggg gtgcggcggc
     2401 ttgagcatct gcccggcata cagcggatgg aaatagggat agttgtctgc catgcgcgaa
     2461 gcggtctgcg cgagcacaga gcgcattgca tcaagtggcg ccacttccag cccggccgca
     2521 ggcaggtgcg tgaaggactt ctgcaattca ggcagaaatt cgttgaggag ctcgagcgcg
     2581 gggtcctgtt tcatcagaaa cagaatacct acagataggc ctcgtccaca tagcaccagc
     2641 gccagtcctc tcccggttcc gcggactgga tgatgggatg ttgcgttgca tggaagtgcg
     2701 cgcgggcatg gcggtttttt gaagagtcgc agcagccgac gtgtccgcat tcaaggcaca
     2761 tgcgcagatg cacccagcgg tctccggtcc tgaggcactc ttcgcagccc ttgctggagg
     2821 gtttcacgtg acggacgtgg tctttgacgt gggtacaagc ggatgccatg gatttgcctc
     2881 ccaggtgcga gca
//
LOCUS       DASRBV010000252         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_70721,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000252 DASRBV010000000
VERSION     DASRBV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70721"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFA02_09240"
     CDS             <1..433
                     /locus_tag="VFA02_09240"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZU10078.1"
                     /translation="FGFPGMRVLQFGFSNRGAHNYLPHRFEKNAVVYTGTHDNDTTRG
                     WWEKGATEVEKAAVEAYVGNVEDGVVWPLVRAASTSVADICLFPVQDILELGSEARMN
                     IPSQSQDNWGWRCPEGVLTPELAKKLADLTEVTDRDGDSSE"
     gene            complement(447..533)
                     /locus_tag="VFA02_09245"
     tRNA            complement(447..533)
                     /locus_tag="VFA02_09245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Leu,seq:taa)
     gene            complement(605..1015)
                     /locus_tag="VFA02_09250"
     CDS             complement(605..1015)
                     /locus_tag="VFA02_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10079.1"
                     /translation="MSASMYIVVEGEDPGFDIFVNGRALARNEDALEKLAFRLGVKPL
                     LDFFSADENSMALLLEEGAGDPEWVKTLPPPQWFSPDEGLETIRTLLDFLRENPLALG
                     LETAPVASELEEYELVLRKTSERNLRWQLAVSWR"
     gene            1299..2288
                     /locus_tag="VFA02_09255"
     CDS             1299..2288
                     /locus_tag="VFA02_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZU10080.1"
                     /translation="MKVRVFYHDKCFDGVCSASLFARFHRECVTPDADYEYHGLVHRA
                     GALFNEADFIGDENAIVDFKYSASPKITWWFDHHLSAFLTPEDHQDYLKGMANGRYSN
                     RRFYDPEYTSCTSFLAHIAETRFGFKAAPVADLVKWADIVDGALYASPQAAVEMAEPA
                     MKLTLIIESTQDPAFTPRLIPFMTEMPLAEVLKQSFVAALLPPLLERHEQALKLIGDR
                     AEYKDGTIYFDITDHQLEGYNKFIPYYLYPEATYSIGLSKSSFRTKVAVGSNPWTKTK
                     PEDMVNLATICERYGGGGHARVGAISFPPDKSDLARKAAAEIVAELRAHHAGK"
     gene            2299..3399
                     /locus_tag="VFA02_09260"
     CDS             2299..3399
                     /locus_tag="VFA02_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZU10081.1"
                     /translation="MKISGAIVIACFCCAAAFAQSGWTIAPFTRPASGNPVITPNPAS
                     VFDDPIAHQLIHWEALHTFNPAAIVRNGKVYVLYRAEDNTGEMQIGMHTSRLGLAESS
                     DGVHFTRSATPVFYPAEDAQKEREWPGGVEDPRIVESEDGTYVLTYTQWNRKTYTVGI
                     ATSRDLLHWTKYGPAFQNAAGGKYKDLKYKSAGIVTKVEHGRLIAARIQGKYWMYWGE
                     GAIHLATSTDLIHWSPVEDAQGNPVELLKARAGHFDSSFPETGPPPVLTRVGIVVIYN
                     GKNAPADGDPSLGPNAYAAGEALFDANDPAHLLAQTGSPVLKPELPYEKTGQYAAGTT
                     FAEGLVLFRGKWLLYYGCADSLVSVAAAPFRK"
     gene            complement(3409..4305)
                     /locus_tag="VFA02_09265"
     CDS             complement(3409..4305)
                     /locus_tag="VFA02_09265"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZU10082.1"
                     /translation="MQAEVKDLLHAGQELTDAALERLLPPETQVPVSIHKAMRHSTFA
                     GGKRLRPVLVMEAARMVRGRGDLPEGIEELGAAIEMLHTYSLIHDDLPALDNDDLRRG
                     KPTCHVVFGEAIAILAGDALQTRAYEVLANLRCPASAIVEIIRLIAQATGTVEGMIGG
                     QVLDLEGEGTKPTAESVDAIHRAKTGALIRVSVVTGGVYAGATSDDMKRLTEFGRRAG
                     LAFQIIDDILDVTQDSAQLGKTAGKDLTSDKATWPAVFGIESSRQQANELIQQAFAEL
                     APYGSAAGGLKSVARYLVERTH"
     gene            complement(4312..>4985)
                     /locus_tag="VFA02_09270"
     CDS             complement(4312..>4985)
                     /locus_tag="VFA02_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10083.1"
                     /translation="ALIGLALRGSFREATTRRSILIRAVTLLALAATASFFTARFFWL
                     PCDEEDNIAAQRATLLNGGFEGTDEYTAAKSDNGDIQQDLPQVRVVNAPDADEADSSV
                     AENPEWKLNTKQSVPAEIKLSLWQTEKKAIEVTTSAPAYALLRLVDYPAWQITINGRT
                     ITTRPHRDDGLMVIPLPSGHSVIAIHYAATPDVKIGRAISLLALCIVLVLYARTKKRQ
                     NLQVS"
BASE COUNT         1034 a         1347 c         1494 g         1110 t
ORIGIN      
        1 atttggcttc cccggaatgc gtgtcctcca gtttggcttt tccaatcgcg gagcgcacaa
       61 ctatctgccg caccgctttg agaagaacgc ggtcgtctac acgggaacgc acgacaatga
      121 cacgacccgc ggctggtggg agaagggcgc cacggaggtg gaaaaggccg cagtagaagc
      181 ctatgttggc aacgtagaag atggggtagt atggccgctt gtccgcgccg ccagtacttc
      241 tgttgccgac atttgccttt ttccggtgca ggacatcctg gaactgggtt cggaagcccg
      301 catgaacatt ccttcacagt cgcaggacaa ctggggctgg cgttgtccgg aaggtgtgct
      361 cacgccggag ctggcaaaga agctggccga tctgacggaa gtcacggaca gggacgggga
      421 ttcttctgag taaatgaggg tagtgttggt gcccggaggg ggacttgaac ccccacggcc
      481 ttgcggcctg cggattttaa gtccgctgcg tctgccgatt tcgccatccg ggcagggaaa
      541 agcgtggccg ctctcatcat cctaaatgga aacgaagaaa agatgcacga tgccggggcc
      601 gcgttcagcg ccagcttact gcgagttgcc agcgcaggtt gcgctcggat gttttacgca
      661 ggacgagttc gtattcttcc agttcactcg ctacgggcgc ggtttccaac cccagagcaa
      721 gaggattttc ccttagaaaa tcaagcaggg tacggattgt ttccagcccc tcatcgggtg
      781 aaaaccattg cggaggtggc agggtcttga cccactccgg atcgcctgca ccttcttcca
      841 gcaggagcgc catggagttt tcgtcggcag agaagaaatc gagcagcggc tttacgccca
      901 gccggaaggc aagtttttcc agtgcatctt cattgcgcgc aagggcgcgt ccgttcacaa
      961 agatgtcaaa tcccgggtct tcaccttcca caacaatgta catggatgcg ctcatgaccg
     1021 ccccagttta ccctacctta gacgtgttgc accgtaggga ggtagcagaa gtcaccttga
     1081 tggtaaccgg ccgtgccggc gtttcgatga accgttggat ttaccggatg gccggaacca
     1141 tgaccgtgcg atgatttgat ttgttcctca gtgccgggat tcatgttttt tcgtgccatt
     1201 cccgggaaac gccctcagct tgacacattg cccattgcca gcaatcctaa gagattcctg
     1261 ctatttatat ttctttattc ttcaggagca ctcatcattt gaaggtacgg gtcttctatc
     1321 acgacaaatg ctttgatgga gtgtgttcag cgtcactgtt tgcgcggttt caccgcgagt
     1381 gtgtcactcc agatgctgat tacgaatacc acgggctggt ccatcgcgca ggggcgttat
     1441 ttaacgaggc cgattttatt ggcgatgaaa atgcaattgt tgatttcaag tattcagcct
     1501 cgcccaaaat tacttggtgg tttgatcacc atctgagcgc gtttcttacg ccggaagacc
     1561 accaggacta tttgaaaggc atggcaaatg ggcgctacag caaccggcgg ttctacgacc
     1621 cggagtacac ctcatgcacc agctttctgg cgcacattgc cgaaacccgt tttggattca
     1681 aggccgctcc agtcgccgac ctggtcaaat gggcagacat tgtagacggc gcgctttacg
     1741 caagtccgca ggccgccgtt gagatggccg agcccgccat gaagctgaca ctgattatcg
     1801 aatccacgca ggacccggcc tttacgcccc ggctgattcc tttcatgacc gagatgcctc
     1861 tggcagaagt gctgaagcag tcctttgtgg ccgcactgct gccgcctctg ctggaaaggc
     1921 atgagcaggc gttaaagctg attggagacc gcgccgaata caaagacggc accatttact
     1981 ttgacattac ggaccaccag cttgagggct acaacaagtt catcccgtac tatctctacc
     2041 ctgaagcaac atattcgatt ggtctttcga agtcgtcctt ccgaacaaaa gtggccgtag
     2101 gctcgaatcc ctggacgaag acgaagccgg aagacatggt gaacctggca accatttgcg
     2161 agcgctatgg cggcggtgga catgcgcgtg tgggggccat cagctttcct ccggacaaga
     2221 gcgacctggc gcgtaaggct gcggctgaga ttgtggcaga gctgcgggcg caccatgcgg
     2281 ggaaatagta cggtgtgtat gaagatatct ggcgcgattg tcatcgcttg tttctgctgc
     2341 gcggcagctt tcgcgcaaag cggctggacc atcgctccct ttacccgccc tgcaagcggc
     2401 aatccggtca ttacgcccaa tcccgcatct gttttcgatg accccatcgc gcaccagctt
     2461 attcattggg aggcattaca caccttcaat cctgcggcga ttgtgcgcaa tggcaaggtc
     2521 tatgtgctct accgcgcaga ggacaatacc ggagaaatgc agatcggaat gcatacctcg
     2581 cgcctggggc tggccgaaag cagcgatggc gtgcatttca cgcgcagtgc aacgcccgta
     2641 ttctatcccg cggaagacgc gcagaaggag cgcgagtggc ccggaggcgt ggaagacccg
     2701 cgcatcgtgg aatcagagga cgggacctac gttctaacct atacgcagtg gaaccgcaag
     2761 acctacaccg tggggattgc cacgtcgcgc gacctgcttc actggacaaa atacggcccg
     2821 gcctttcaga atgcggcagg cggcaaatac aaagacctga aatacaaatc agccggaatt
     2881 gtcacgaaag tagaacatgg ccggctgatc gcggcgcgta tccagggaaa gtactggatg
     2941 tattggggag aaggtgcaat tcatcttgcc acgtcaacgg acctgattca ttggtctccc
     3001 gttgaagacg cccagggaaa tccggtcgag ctgctgaaag cgcgggctgg acatttcgac
     3061 agcagcttcc ctgagaccgg tcctccgccc gtgctgacgc gggtgggaat tgtggtcatt
     3121 tacaacggca aaaatgcgcc agcggatggc gacccatcgc ttggcccgaa tgcctatgcg
     3181 gcaggtgagg cgctcttcga tgcaaacgat cctgcgcatc tgctggcaca gacgggcagt
     3241 ccagttctga aaccagaact gccctacgag aaaacagggc agtacgcggc gggaaccacc
     3301 ttcgccgaag ggcttgtgct cttcagggga aagtggcttc tctactacgg atgcgccgac
     3361 tcgctggtct ccgtggccgc ggccccgttc agaaagtagt tcttcggatt aatgggtgcg
     3421 ttctacgaga tagcgggcaa cggattttaa tccgccggca gcggaaccgt aaggcgcaag
     3481 ttctgcgaat gcctgctgga tgagctcatt tgcctgctgg cgagaggatt caattccgaa
     3541 gacggccggc caggttgctt tatcgctggt caggtctttt ccggcggtct tgccgagctg
     3601 cgccgaatcc tgcgtgacgt ccagaatgtc atcaatgatc tggaaggcaa gcccggccct
     3661 gcggccaaat tcggtcaggc gcttcatgtc gtctgaagtg gcgcctgcat acacgcctcc
     3721 ggtgacgacg ctgacgcgaa tcagcgctcc ggtcttggcg cgatggatgg catctacgga
     3781 ttcggcagtt ggtttggtgc cttcgccttc gaggtcgagc acctgtccgc caatcatgcc
     3841 ttcgaccgtg cctgtggcct gcgcgatgag gcggatgatc tcgactatgg ctgaggctgg
     3901 gcagcgcaga ttggccagga cttcataggc gcgggtctgg agcgcgtctc cggccaggat
     3961 ggcaatggct tctccgaaga caacgtgaca ggtgggcttg ccgcgccgca ggtcgtcatt
     4021 gtcgagcgcg ggcaggtcgt catggataag cgaataagtg tgcagcatct caatggctgc
     4081 gcccagttct tcgattcctt caggcaaatc gcctctgccg cggaccatgc gggcggcttc
     4141 cattacgagc acggggcgaa ggcgctttcc accggcaaag gtgctgtggc gcatggcctt
     4201 gtgtatggaa acggggactt gcgtctctgg cggcaacaac cgctcgaggg cggcatccgt
     4261 gagttcctgg cctgcgtgca gcaggtcttt tacttcggct tgcatttctt ttcatgatac
     4321 ctgaaggttt tgccgtttct ttgttcgcgc gtaaagtacg aggacgatgc agagagcaag
     4381 cagagaaatg gcgcggccga tctttacgtc cggcgtagcg gcgtaatgga tggcgatgac
     4441 agagtgtccg gatggaagcg gaatgaccat caggccgtcg tcacggtgcg ggcgtgtggt
     4501 gatggtcctt ccattgattg tgatctgcca tgcgggatag tccacgaggc gaagaagggc
     4561 gtaggcaggg gcagatgtgg tgacttcgat ggccttcttt tctgtttgcc agagagaaag
     4621 ttttatctct gccggaacgc tttgtttcgt gtttagcttc cattccggat tttcagccac
     4681 agagctgtct gcttcatccg catcgggagc attgacgacg cggacctgcg gcaagtcctg
     4741 ctgaatgtcg ccgttatctg atttcgcggc agtgtattcg tctgtacctt caaatccgcc
     4801 gttgagaagt gtggcgcgtt gcgcggcgat gttgtcttcc tcatcgcatg gcagccagaa
     4861 gaacctggct gtaaaaaaag aggccgttgc tgcaagtgca agcagcgtta cggcgcgtat
     4921 caggatgctt ctgcgggttg ttgcttcgcg gaagctgccg cggagggcga gtccgatgag
     4981 ggcag
//
LOCUS       DASRBV010000253        11698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_577644, whole genome shotgun sequence.
ACCESSION   DASRBV010000253 DASRBV010000000
VERSION     DASRBV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577644"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VFA02_09275"
     CDS             complement(<1..1270)
                     /locus_tag="VFA02_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10084.1"
                     /translation="MRISFRFPHTLSSNATRQHRRRSLLYACALIAVLPLAWRGVSCG
                     HDFDFHLQSWLEAVQHWREGVLYPHWDASANYGAGEPRFVFYPPVTWMLGALLGTLLP
                     WNWVPIAFTLLAFIACGLSFYKMACEWMPEDAAGVAACIYLLNPYLLFVAYERTAYGE
                     LLSAALLPLLVLYGLRKQPSLLPLSLVIVALWLTNAPSAVMGCYFLALIVLVGAVCQK
                     MTGTPSGPASIHLIFRATGALFLGLALAAFYIVPAYYEQRWVEIARAIAPGMRIEDSF
                     LFEHTGAIYHDQVLRTASWIAVAMLAATAIAAWFSGLTTHLRSGLEGHNSRTIFRTPL
                     IITAIVILLLLLPFSDPLWRHLPELKFLQFPWRWLLVLGVAMSALIGLALRGSFREAT
                     TRRSILIRAVTLLALAATASFFTARFFWLPC"
     gene            complement(1267..3699)
                     /locus_tag="VFA02_09280"
     CDS             complement(1267..3699)
                     /locus_tag="VFA02_09280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZU10085.1"
                     /translation="MPLGRTVRLEEEKRSHPFLRLFLGFLLLVFLAVAVGVLGGAWWL
                     KHAMRDSLPQLDGDMHLPGLSAPVTVRRDQHGVPHIEAATLDDLFEAQGYITAQDRLW
                     QMDMARRMAAGEAAEILGKRLVDHDRLERVLQMRATAERMASSLPERDRRFFEDYSRG
                     VNEYIDSHRDHLPAEFRLLMYKPRAWQPVDSILIGLSMVQILDEHWPEKLEREKVEAR
                     IGPTLAADLYPTGSWRDHPPTVTPPDLTAPQPVIPQVPLDESQSGLRDLLHLREVVQG
                     TQEICRECIPGSNEWAVAGEHTASGKPILSNDMHLDHGIPNTWYEIDLKSGNFHAAGV
                     TIAGMPFVTAGHNDHIAWGYTALYGDTQDVYVEKTNSQGDYLGTDGWHPIEHTQETIR
                     VRGSSDVTVDIERTEHGPVLNPLLPSEKRTLSLRWTAYDPKAAAIPLFELDTASNWDE
                     FRQAMANWWGPTLNVVYADDQGHIGYQAVGFIPNRPNGLKGVPISDNQHEWQDFVPFA
                     QMPGTLDPANGILATANSRITPDNYPYQLTLEWASPYRNERIWKWLSSKDKLTPADML
                     KLQTDIYSEVDQEFAQRFAYAIDHSSNADARLRQAADLMRTWNGEVTIDSAPAAIVAA
                     AKHVFWPMLLKPKLGDDWKLYQWAESAFAQEELIMHAPAQWLPRNYATWDDFLAACVA
                     EGLKEDHAPSDLSRWRYGYAHPVELVHPLYNLLPWFKNWTGTGVQPQSGDTTTVKQVG
                     RTFGPSQRFTMDWSNVDSSTENIVMGESGDPLSPYYRDQWWFWYNGRTFALPFTNAGI
                     NASTTHTLRLLP"
     gene            3836..4762
                     /locus_tag="VFA02_09285"
     CDS             3836..4762
                     /locus_tag="VFA02_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZU10086.1"
                     /translation="MQTTDIVAQKEQVSSLGGYGACALAGCFWGMGFFFGKIVLAEMS
                     VAHMVLYRFWFACPPLFFVLRRPRFSGREWQLLLVAAFLGIPVQYLVQFQGLSLTTVS
                     HAALMVGTMPVILAMGACIFAHERLDRLGWLALAGSTAGVAMIVLSSNHAVSAHGPTL
                     AGDLLVVLSLLIALAWVLLNKHLMRTHSPLQVTAYGLLSGTLMLSLWVLLRNGLPPAH
                     GVSAHAWLALLASGLLCNTSAMFLWNWGISRVPASRAGVFLNIEPAMGSMLGVRLLGE
                     RLSPLAWIGGGLIVTAAIMITSGGRKADSNPL"
     gene            4922..5668
                     /gene="aqpZ"
                     /locus_tag="VFA02_09290"
     CDS             4922..5668
                     /gene="aqpZ"
                     /locus_tag="VFA02_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HZU10087.1"
                     /translation="MSSLVRRSIAEFLGTFWLVFGGCGAALISAAFPELGIGFLGVAF
                     AFGLTLLTMAYAIGHISGCHINPAVTCGLVAGKRFPVKDLPAYVVAQILGGIAAAAVL
                     YLIVTGNPTFDATSSTFASNGYGAHSPGNYSLLSCFVAEFVLTFFFLVVIMGATDKRV
                     PAGVAPIAIGLVLTLIHLISIPVTNTSVNPARSTGPAIFAGYWWPLQQLWLFWVAPIL
                     GAVAGGFAYRFLMEEKEESVEALDIERAGK"
     gene            complement(5783..5932)
                     /locus_tag="VFA02_09295"
     CDS             complement(5783..5932)
                     /locus_tag="VFA02_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZU10088.1"
                     /translation="MFLVLALILILIWIGSFVVYHVTGFLIHLLLIFAVISFILHFVR
                     GKKST"
     gene            complement(5994..6470)
                     /locus_tag="VFA02_09300"
     CDS             complement(5994..6470)
                     /locus_tag="VFA02_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZU10089.1"
                     /translation="MRELLLDELQDLHSAEVQITKALPKMVKTASNEALKEAFNNHLK
                     ETENQVQRLEKIFKHLKENPKGKTCEGMKGILKEGEERIKEGGGPEVVDAGLISAAQR
                     VEHYEIASYGSARAYAEMLGEVEVVRLLEETLEEEKAADQKLTKLSGRINQQAKAA"
     gene            6605..7555
                     /locus_tag="VFA02_09305"
     CDS             6605..7555
                     /locus_tag="VFA02_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU10090.1"
                     /translation="MRLLVLAFVLISFDVSAQVLQNTNYQRELEHAKSLLAGMTPTDV
                     PHHIHYDLKFYDRNGHENTATYDIYRDPVMYERTEVKSGNYQLTQISNKRDQKTWQHY
                     TGDKPLKIAEFEQATDRPSVAIDRFALELDRIDNMQPQKLEGMPLLCANDNAGTAICF
                     NPFIGLFAYAQMFNQTVMYDNWLPVGSHTVPGNIRIFQDKKLLLEASGTVEVVKKFPP
                     HFMEVPDTPSQPDPASLHKVLQSKGMDLTQPRFGNASLRLSVDENGHVAKVEIVDSDD
                     KRLEGPARKFAQHLIFAPQMENGRAVPFETVIYVVYYPFF"
     gene            complement(7540..7809)
                     /locus_tag="VFA02_09310"
     CDS             complement(7540..7809)
                     /locus_tag="VFA02_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10091.1"
                     /translation="MFLRKLQVLRTTPDGLSQPCPVAWIDNFAMRNFTNDAVFDDTLP
                     IGDGLLEAGFRVPLDRLQSAMEDWFRRKGYLKPDEHVRIVDQKNG"
     gene            complement(7862..9235)
                     /gene="rseP"
                     /locus_tag="VFA02_09315"
     CDS             complement(7862..9235)
                     /gene="rseP"
                     /locus_tag="VFA02_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZU10092.1"
                     /translation="MPFAITAIVSMLVVLGIMVLVHEFGHFIVAKLFGVRVEVFSIGF
                     GTRLFGFRRGDTDYRLSLLPLGGYVKMSGETPGETRTGDPGEFSSHPRWQRILIGLAG
                     PAANFALAFVLMTGYYMMHNEVDQYLSGPAVVDFVPQNSAAAHAGIQSGDRIVKFDKD
                     VNPTWEKVRIRAALDVNSTVPVTVERNVDGQTQQFSTQLYLLDGSKGDDFEVESIGLI
                     PRYQNGPIKVHDVEPGYPAEKAGLKAGDEILAIDGVQLHWTSAVVAYLQQNGSKPVTL
                     TVQRAGKTLQLTMAPALGDDGTGHQSYRLGFKPYLPPFHVEQLPLPQAVRHSVSFNLQ
                     NSGLILDVLHRLITRQSNMQQLSGPIGIARQTGEAISMPGWQPIITLMALISLNLGIF
                     NLLPIPILDGGMILLLLIEGTIRRDLNQQLKERIYQVAFVVLVIFAVFVMFNDVSKFN
                     LFSKLKP"
     gene            complement(9254..10438)
                     /locus_tag="VFA02_09320"
     CDS             complement(9254..10438)
                     /locus_tag="VFA02_09320"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZU10093.1"
                     /translation="MKHIAILGSTGSIGTSTLDIIEQYPERFAVASLAAGRNLDAVFQ
                     QCERWRPRVVSVATEELATTLAARLKSAGISGIETVFGPEGTVLVATLPEVDFVVSAI
                     VGVAGLEATYAAVEAGKAIGLANKEALVAAGEILTAAARRKNVPLLPIDSEHNAVHQC
                     MRAGTKHEVRRIWLTASGGPFRTLPLDRFDSITVEQALKHPTWVMGRRITIDSATMLN
                     KGLEIIEACRLFDVPPSTVRVTIHPQSTVHSLVEYIDGSILAQISVTDMRLPILYAMS
                     YPERVSSDLTFDMSALGQLCFEPPDFARFPCLRLAYEAAEKGGAHCIALNAADEIAVE
                     AFLNKSIPFTGIARTIEEVLQATPEAHPATIHEVLASDRKARILAREALIRAGYAVSD
                     AR"
     gene            complement(10540..11076)
                     /locus_tag="VFA02_09325"
     CDS             complement(10540..11076)
                     /locus_tag="VFA02_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU10094.1"
                     /translation="MKKIAFFTVLFLACSAYSQTTPAEKPTLRSILLAQMKETHNQKE
                     WFVPANVAVAGLTADQANWKDGKGNHSVGQLAYHLVYWDTRSLEKFKGEPTPKFSGNN
                     DETFDNFDSKKWDETVRQLDAVLTEWEKAIATADDAKLEQWASDIEKISAHNAYHIGQ
                     IIYVRKEQGSWNPENGVK"
     gene            complement(11195..>11698)
                     /locus_tag="VFA02_09330"
     CDS             complement(11195..>11698)
                     /locus_tag="VFA02_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HZU10095.1"
                     /translation="MADMYLMAVERLAASGLRQYEISNFARAGAESAHNQKYWLRRPY
                     LGLGVDAHSMLRAADGTAIRFATTDNLEDYLQSPGWDDRHALSRQEELEEAWFLGLRL
                     NAGVSLKVLEAEFGAEAIAGFRPTLNVLAEEGFVQQSGETIQLTPQGRLLSNNVFERF
                     LLPAAAA"
BASE COUNT         2491 a         3016 c         3489 g         2702 t
ORIGIN      
        1 cgcatggcag ccagaagaac ctggctgtaa aaaaagaggc cgttgctgca agtgcaagca
       61 gcgttacggc gcgtatcagg atgcttctgc gggttgttgc ttcgcggaag ctgccgcgga
      121 gggcgagtcc gatgagggca gacatcgcca caccgagaac cagtagccat cgccacggga
      181 actggagaaa tttgagttcc ggaaggtgcc gccagagtgg gtcgctaaaa ggaaggagca
      241 gcaggagaat gactatcgcg gtgatgatca ggggtgtccg gaagatggtc cgcgagttgt
      301 gaccttccag tcctgacctc agatgggtag tcaggcctga gaaccatgct gcgatcgctg
      361 tagccgccag catggctacg gcaatccatg aagcggtgcg cagtacctga tcgtggtaga
      421 tcgctccggt gtgttcaaag aggaagctgt cttcaatgcg cattccagga gcgatggcgc
      481 gcgcgatttc tacccagcgc tgctcgtaat aagcaggaac aatgtagaaa gcagccagtg
      541 cgaggcccag gaaaagcgcg cctgttgcac gaaagatgag atggatcgag gccggccccg
      601 atggtgtacc cgtcatcttt tggcacacag cgccaacgag cacaatcagc gcaagaaaat
      661 agcagcccat cacggcggat ggagcgttcg tcagccatag cgccacgatg acgagcgaaa
      721 gcggcagcag gctgggttgt ttccgcagcc catataggac aagcagtgga agcagggctg
      781 cggagagcaa ttcaccataa gcggtacgct cgtaggcaac gaacagaaga tatgggttga
      841 gcaggtaaat gcaggctgcg actccggcgg cgtcctccgg catccactcg caggccattt
      901 tgtagaagct gagtccgcag gcgatgaacg caagcaaagt gaatgcaata ggcacccagt
      961 tccagggcag cagtgtgccg agcaaagcac ccagcatcca tgtaaccggc ggataaaaga
     1021 cgaagcgcgg ctctcctgct ccgtaattcg cagatgcgtc ccagtgcgga tagagcacgc
     1081 cttcgcgcca gtgctgcacg gcttccagcc aggattgcag gtggaaatca aagtcgtgtc
     1141 cacaggagac cccacgccat gccagtggga ggacggcaat cagcgcacag gcatagagca
     1201 gcgaacgcct gcgatgctgt cgcgtggcgt tactcgataa agtatgcgga aagcgaaacg
     1261 aaatcctcac ggcagcagtc gcagagtgtg tgtggtggaa gcgttgatac cggcattggt
     1321 aaacggcagt gcaaaggttc tgccgttgta ccagaaccac cactggtcgc ggtaatacgg
     1381 gctgagcggg tcgccggatt cgcccatcac aatattttct gtggagctat ctacgttgct
     1441 ccaatccatg gtgaagcgct gcgatggacc gaaggtgcgg cctacctgct tgacagtggt
     1501 agtgtcgccg gactgcggct gcactccagt gcctgtccag tttttgaacc agggcagcag
     1561 gttataaagc ggatgcacca gctcgactgg atgcgcatag ccatagcgcc atctgctcag
     1621 gtcagagggg gcgtggtctt ccttgagtcc ttccgccacg catgccgcca gaaagtcgtc
     1681 ccatgtggcg tagttcctgg ggagccattg cgcaggcgcg tgcatgatga gttcttcctg
     1741 cgcgaaggcg ctttcggccc attggtagag tttccagtca tcgccaagtt ttggcttcaa
     1801 cagcatgggc cagaagacat gcttggctgc tgcgacaatg gctgccgggg cagaatctat
     1861 cgtgacctcg ccgttccatg ttcgcatcag gtcggcggcc tggcgaagtc gcgcatcggc
     1921 attgctggag tggtcaatgg cataagcgaa gcgttgcgcg aactcctggt ctacctcgga
     1981 atagatgtca gtttgcagct tcagcatgtc ggcgggcgtg agtttgtctt tgctggaaag
     2041 ccatttccag atgcgctcat tacggtaagg gctggcccat tcgagcgtga gctgatacgg
     2101 gtagttgtcc ggagtaatgc gcgagtttgc tgttgccaga atgccatttg ccggatcaag
     2161 cgtgccgggc atctgcgcaa atggaacaaa gtcctgccat tcgtgctgat tatctgagat
     2221 ggggacgccc ttcaggccat tgggacggtt gggaatgaat cccacggcct ggtagccgat
     2281 gtgaccttgg tcgtctgcat agacgacgtt gagcgtaggc ccccaccaat tggccatggc
     2341 ctgacggaat tcgtcccaat tgctggccgt atcaagctca aagagaggaa tggccgcggc
     2401 tttggggtca taagcggtcc agcggagcga gagggtgcgc ttttcactgg gaagcaaagg
     2461 attcagcacc gggccatgtt cggtgcgttc gatatctaca gtaacgtcac tgctgccgcg
     2521 cacgcggatg gtttcctgcg tatgttcgat ggggtgccag ccatctgtgc ccagataatc
     2581 gccctggctg ttggttttct ccacgtagac gtcctgcgta tcgccataga gggcggtgta
     2641 tccccaggcg atgtggtcgt tgtgtccggc tgtgacaaac ggcatacctg cgatagtgac
     2701 tccggcagcg tgaaagtttc ctgacttcag gtcaatttca taccaggtgt tcgggatgcc
     2761 gtggtcgaga tgcatgtcat tcgacagaat cggctttccg ctcgccgtat gctctccggc
     2821 aacggcccac tcgtttgaac cgggaatgca ctcgcggcag atttcctgcg taccctgaac
     2881 cacttcgcgc agatggagca ggtcacgcag accggactgc gattcatcca gaggcacttg
     2941 tggaatgacc ggctgcggcg cagtcaggtc gggcggcgtt accgtaggtg gatggtcgcg
     3001 ccacgatccg gtggggtaga ggtcggcagc aagtgttggt ccaatgcgag cctccacttt
     3061 ttcacgctcc agtttttctg gccagtgctc gtccagtatc tgcaccatgc tcaggccgat
     3121 gaggatggaa tccactggct gccatgcgcg cggtttgtac atgagcagac gaaactcggc
     3181 agggaggtgg tcgcggtggc tgtctatgta ttcgttgacg ccgcgggagt agtcctcaaa
     3241 gaagcggcgg tcgcgctcgg gaaggctgga ggccatgcgt tcggccgtgg cgcgcatctg
     3301 gaggacgcgc tccaggcggt cgtggtctac gaggcgcttg cccagaattt ccgcggcctc
     3361 gcctgcggcc atacggcggg ccatgtccat ctgccagagc cggtcctgcg cggtgatata
     3421 gccttgcgcc tcaaatagat cgtcgagtgt ggccgcttca atgtgcggga cgccgtgctg
     3481 atcgcgtcgc acggtcacag gagcagacag cccgggcaga tgcatgtcgc catcgagctg
     3541 aggcagggaa tcgcgcatgg catgtttgag ccaccatgct cctccgagga caccaacggc
     3601 cacagcaaga aacaccagca agagaaaacc caaaaacaag cgcagaaagg gatggctgcg
     3661 cttttcttct tcaagacgaa cagttctacc aagaggcata ccgaagacat tgtctcatcg
     3721 gaaggagcgg ttttgcgcag gacaggaaga gggtgaataa tttggccggc tgccatacat
     3781 tggcagcgaa atatttttcg gcgcttccag gtatgctttg atgaatcaaa gttctatgca
     3841 gaccaccgac atcgttgcgc aaaaagagca ggtatcttca cttggcgggt atggtgcgtg
     3901 tgcgctggcg ggatgtttct gggggatggg gttctttttt ggcaagattg tgctggcgga
     3961 aatgtccgtg gcacacatgg tgctgtaccg tttctggttt gcatgtccac ccctgttctt
     4021 tgtgctgcgg cgcccgcggt tttccggcag ggaatggcag cttttactgg tggctgcgtt
     4081 tcttgggatt ccggtgcagt atctggtgca atttcagggg ctgtcgctga cgacagtaag
     4141 ccacgccgcg ctgatggtgg gcaccatgcc agtgatcctg gccatggggg catgtatctt
     4201 tgcgcatgaa aggctggacc gtctcggctg gcttgccctt gccgggtcaa ctgcgggagt
     4261 ggccatgatt gtgttgagca gtaaccatgc tgtttctgcg catgggccga cactggcagg
     4321 cgatttgctt gttgtgcttt ctctgctgat tgccctggcg tgggtcctgt tgaacaagca
     4381 cttgatgcgg acacattctc cattgcaggt gacggcatat ggtttgttga gcggaacgct
     4441 gatgctgtcg ctatgggtgc tgctgcgcaa tggtctgcct ccggcacatg gcgtgagtgc
     4501 ccatgcttgg ctggcgttgc tggccagcgg cctgctttgc aacacaagcg cgatgttttt
     4561 gtggaactgg ggcatctccc gcgtacctgc gtcgcgggca ggtgtttttc tgaatatcga
     4621 gccagcgatg ggttccatgc tcggtgtcag gctgctgggg gaaaggctca gtcctttggc
     4681 gtggattggg ggcgggctga ttgttaccgc agccattatg atcacgagtg ggggccggaa
     4741 agcagacagt aatcctttat gatttaaaca tttgcgaagt ggtccttgaa gaaaccggtt
     4801 taaaaacggt tccttattac ggaagctaag caaaaacttt cgctgatgaa atgcttctgt
     4861 gataacgtct ttgcggattc agaactcgct gtcatggcgt aatgtaaagg aggattcggc
     4921 tgtgtcgtca ctcgtcaggc gttctattgc agagtttctg ggtactttct ggctggtctt
     4981 cggtggttgc ggagcagctt tgatttcagc ggcctttcct gaacttggga ttggctttct
     5041 gggtgtggca tttgcctttg gtctgacgtt gctgaccatg gcctatgcca ttggacacat
     5101 ttccggctgc cacatcaatc cagcagtcac ctgcggttta gtcgcaggca aacggtttcc
     5161 ggtaaaggac ctgcctgcct atgtggtggc gcagatactt ggtgggattg cggctgctgc
     5221 tgtgctttat ctgattgtga cgggaaatcc gaccttcgat gccacctcca gcaccttcgc
     5281 ttctaacggc tatggagcac attctccggg gaattattcg ctgttgtcct gctttgtggc
     5341 cgagtttgta ctgacgttct ttttcctggt ggtcatcatg ggagcaaccg acaagcgcgt
     5401 tcctgccgga gttgcgccca tcgcgattgg tcttgtgctt acgttgattc acctcatcag
     5461 cattccggtt acgaatacct cagtcaatcc ggcgcgcagc accggtcctg caatttttgc
     5521 cggatactgg tggcccctgc agcagctctg gctgttctgg gtggcaccga ttctgggagc
     5581 tgtggccggc ggatttgcct atcgtttcct gatggaagag aaggaagaaa gcgttgaggc
     5641 gctcgacata gagcgggcgg gaaaatagcg cataggttca gccgtcaggt ttgttttgaa
     5701 ggcgctggag atttccggcg ccttttttgt gactgagagt ggcgacgacc catttgcaac
     5761 agcagtgtgt tgagcgctcg ctttatgtgc tttttttgcc gcggacaaaa tgcagaataa
     5821 atgagatcac ggcaaagatg agcagaaggt ggatcaaaaa cccggtgacg tgatagacga
     5881 caaaactgcc tatccaaatc agaatcagaa tcaatgcaag aacaagaaac atggcctacc
     5941 tttctttcag aatggacagg gaaggccatg atgaccctcc ctgcatgatg tatttatgct
     6001 gctttggcct gctggttgat ccggccggac agctttgtca gtttctggtc cgcggccttt
     6061 tcttcttcca gagtttcctc aagcaggcgt acaacttcga cttctcccag catttcagcg
     6121 tatgcgcggg ccgagccata ggaagcaatt tcgtagtgtt ctacacgctg ggcagcggaa
     6181 ataagccctg cgtccaccac ttccggcccg ccaccctctt tgatgcgctc ttcgccttcc
     6241 ttgagaatgc ctttcatgcc ttcgcaggtc ttgccctttg gattttcctt caagtgtttg
     6301 aatatctttt ccagccgctg cacctggttt tcggtctctt tcaggtggtt gttgaaggcc
     6361 tctttgagtg cctcgttgga agcagttttc accattttag gaagggcctt ggtaatttgc
     6421 acctcggccg agtgcaggtc ctgcagttcg tccagcagaa gctctctcat actttccagt
     6481 ttcatcgcaa cacctcggtc attgagaaat tgacgaacat tatctgtgat gcaatttcga
     6541 ggcatagtga aacgcgtcct ggacagttgc ccgaaagcga gaattcccat taagatagcg
     6601 cccgatgcgc cttttggtcc tggcttttgt gctgatctca ttcgatgtaa gcgcccaggt
     6661 gttgcagaac acgaattatc agcgcgagct cgaacatgca aagtcacttt tggctggcat
     6721 gaccccgaca gatgtccctc atcacattca ttacgacctg aagttttatg accgcaatgg
     6781 gcacgaaaat acggccacgt atgacattta ccgtgatccg gtgatgtatg agcggacaga
     6841 ggtgaagtca ggcaactatc agttgacgca gatcagcaac aagcgggacc agaagacatg
     6901 gcagcattac acaggtgata agcccctgaa aattgccgaa tttgagcagg caacggacag
     6961 gccatctgtg gccatagacc gctttgcgct tgagttggac cgcattgata atatgcagcc
     7021 acaaaaactc gaaggaatgc ccttgctttg cgcgaacgac aacgcaggga cggccatctg
     7081 cttcaatcct tttatcgggc tgtttgccta cgcgcagatg tttaatcaaa cggtgatgta
     7141 cgacaactgg cttccggtag gctcgcatac ggtgccggga aacattcgca tttttcagga
     7201 caaaaagctg ctgctggaag cgtccggaac ggtcgaagtg gtgaagaagt tcccgccgca
     7261 ttttatggag gtgccggaca caccttcgca gcctgaccct gcctcgctgc acaaggtgct
     7321 ccagagcaaa gggatggact tgacgcagcc acgttttgga aatgcctcgc tgcgcctctc
     7381 agtggacgaa aacgggcatg tagcgaaggt cgagattgtg gattcggatg ataagcgcct
     7441 ggaaggccct gcgcgaaaat ttgcgcaaca tctgattttt gcgccacaga tggagaatgg
     7501 ccgcgccgtt ccgtttgaga cggtgattta cgttgtgtat tatccgttct tctgatccac
     7561 aatacgcacg tgttcatctg gcttcagata gcctttgcgg cgaaaccagt cttccattgc
     7621 ggattgcagg cgatcgaggg ggacgcggaa ccctgcttcc agcaggccgt cgccgatggg
     7681 aagcgtgtcg tcaaaaacgg catcatttgt gaagttgcgc atggcgaagt tgtctatcca
     7741 tgcaaccgga cagggttggg aaagcccatc aggagtagtg cggaggacct gtaacttgcg
     7801 aaggaacatg gttcgcagtt tacgtcaggt gtgccatgcc tgatacacct gacgaagtag
     7861 tttacggctt gagctttgag aatagattga acttcgagac gtcgttgaac attacaaaga
     7921 cagcgaagat caccagcacc acaaacgcta cctgatagat gcgctctttc aattgctggt
     7981 tgaggtcgcg gcggatagtg ccctcgatga gcagaagcag aatcatgccg ccgtcaagga
     8041 tcggaatcgg cagcagattg aagatgccga ggttcaggct gatcagggcc atcagggtga
     8101 tgataggctg ccatccaggc atggaaatgg cctcgcctgt ctgccgggca atgccaatcg
     8161 gaccgctcaa ctgctgcata ttagactggc gagtaatgag ccggtgcagc acatcgagaa
     8221 tcaagccgga attttgcagg ttgaagctca cggaatggcg taccgcttgt gggagcggca
     8281 attgctccac atgaaacgga ggcaggtagg gtttgaatcc aagcctgtag ctctggtgtc
     8341 cggtgccatc gtctcccagc gcaggggcca tggtgagttg cagcgttttt ccagcgcgct
     8401 ggacggtgag tgtcaccggc ttgcttccat tctgttgcag gtaggcaacg acagcagagg
     8461 tccagtggag ttgcacgcca tcaatggcga gaatttcatc gcctgccttc agtccggcct
     8521 tttctgcggg gtagcctggc tcgacatcat gtactttgat ggggccgttc tggtaacgcg
     8581 gaatcaggcc tatggattcg acctcgaagt cgtcaccctt gctgccgtcc agcaagtaga
     8641 gctgagttga gaattgctgt gtctggccgt ccacattccg ttcaaccgtt acgggcacgg
     8701 tggaattgac gtccagagcg gcgcgaatgc ggactttctc ccaggtaggg tttacgtctt
     8761 tgtcaaactt tacgatgcga tcgccagact gaataccggc atgagcagca gctgagttct
     8821 gcggaacgaa atcaacgacg gcggggccgg acagatactg gtccacctcg ttatgcatca
     8881 tgtagtagcc cgtcatgagg acaaaagcga gggcgaaatt ggcagccgga ccagccagcc
     8941 caatcagaat gcgctgccag cgcggatgcg aagagaactc gcctggatct ccggtacggg
     9001 tctcgcccgg tgtttcgccg gacattttta catagccgcc gagaggcaaa aggctgaggc
     9061 ggtagtcggt atcaccgcga cgaaaaccga aaagccgcgt gccgaagccg atggagaaaa
     9121 cttctacgcg aacgccaaat aatttggcca cgatgaagtg gccaaactcg tggacgagca
     9181 ccatgatgcc gagcaccacg agcatggaaa caattgcggt aattgcaaag ggcatgatct
     9241 ttaagaaaac ctgtcagcgg gcgtcagaga cggcgtagcc ggccctgatc agggcctccc
     9301 tggccaaaat tcgcgccttt ctatctgatg ccagcacttc atgaatggtc gcagggtggg
     9361 cctcgggggt ggcctgcagt acttcttcta ttgtacgcgc gattccggta aaaggaatgg
     9421 atttattcag aaacgcctcg actgcaattt catcggctgc attgagagca atgcagtggg
     9481 cgccaccttt ttctgcggcc tcataggcaa ggcgaagaca agggaaacgg gcgaagtcag
     9541 gcggttcaaa acagagctgt ccgagtgcag acatgtcgaa ggtcaggtca gaagagaccc
     9601 gctctgggta gctcatcgca tacaggatgg gaagacgcat gtcggtaacg gaaatctgcg
     9661 ccaaaatact tccgtcaatg tactcgacca gcgaatgcac ggtagattgc ggatgaatgg
     9721 tgactctgac tgtagacggc gggacatcaa aaagccggca cgcttcgatg atctcaagcc
     9781 ctttgttcag catggtggct gagtcaatcg tgatacgccg ccccatgacc catgtagggt
     9841 gtttgagggc ctgctctact gtgatggaat caaagcgatc aagaggcagg gtccggaatg
     9901 ggcctcctga agcagtgagc cagatgcggc gcacttcgtg ttttgtgccc gccctcatgc
     9961 attggtgaac ggcgttgtgt tcagaatcaa tgggcagcaa cggaacattt ttcctgcgtg
    10021 cggccgcggt aagaatttct ccggcagcca cgagcgcttc tttatttgca aggccgatgg
    10081 cctttcctgc ttcgacggcg gcgtaggtgg cttccagccc ggcaacacca acgatggcgg
    10141 aaacaacaaa gtctacttcc ggcagagtgg caacgaggac tgtgccctct gggccgaata
    10201 cggtttcaat tccggaaatg cccgcgctct tgagacgcgc agccagtgtt gtggcgagct
    10261 cttccgtagc aacggaaacc acacggggcc gccagcgctc gcactgttga aaaacagcgt
    10321 cgagattgcg gcctgctgcg agagatgcta cggcaaagcg ttccgggtat tgttcgatga
    10381 tgtcgagggt gctggttccg atagaccctg tcgagccgag aattgcgatg tgtttcaagc
    10441 gtgtacgcgt ccctgacctg aagatatttt ataggtttgg acgcaatatg tgacggacgt
    10501 ccttctgcgt gcgggaccga aaagacgtcc ggcagggact tacttcacgc cattttccgg
    10561 gttccaggag ccttgctctt tacgcacata aatgatctgt ccgatgtgat aggcattgtg
    10621 cgcgctgatt ttctcaatat ctgaggccca ctgttccagc tttgcatcgt cggcagtcgc
    10681 gatggctttt tcccactcgg tcaggaccgc gtcgagctgg cggacggtct cgtcccactt
    10741 tttgctgtcg aagttgtcga aggtctcgtc gttgtttccg ctgaatttgg gggttggttc
    10801 gcctttgaac ttctccagac tgcgcgtgtc ccagtagacg aggtgataag cgagctggcc
    10861 gacggagtgg tttcccttac cgtctttcca attggcctgg tccgcagtca gacccgccac
    10921 ggccacattg gcgggcacaa accactcttt ctggttgtgg gtttctttca tctgtgcaag
    10981 cagaatgctg cggagcgtgg gcttctccgc cggcgtggtt tgtgaatacg cggaacaggc
    11041 cagaaatagg accgtaaaaa atgcaatctt cttcatagca gcttaccctc tcactttcag
    11101 tgcaaaggct actctggcag ttctggtttg tcagtcaatt tcagccgcat cgtttgactg
    11161 cgaagacaaa gggccttttc tttgcgccca ttcgttaagc tgcagcggcg ggaagcagaa
    11221 agcgttcaaa gacattgttg gagagcaagc gtccctgtgg ggtcaattgg attgtttcgc
    11281 cggattgctg cacaaagccc tcttcggcca ggacgttgag tgtgggccgg aaacctgcga
    11341 tggcttctgc tccgaactcc gcttccagca ccttcaaaga gactcccgca ttcagacgca
    11401 gtcccagaaa ccatgcctct tccaactctt cctggcggga gagggcgtgg cggtcgtccc
    11461 acccgggcga ttgcagatag tcttcgagat tgtccgttgt ggcaaagcgg atggcggtgc
    11521 catctgctgc gcgcagcatg gaatgcgcat ctactcccag acccagataa ggcctgcgca
    11581 gccagtattt ttgattgtgt gcggattcgg ctccagcgcg ggcgaaattc gagatttcat
    11641 attgccgaag cccgctggcg gcgagccgct ccaccgccat caggtacatg tcagccat
//
LOCUS       DASRBV010000254        12090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_166326, whole genome shotgun sequence.
ACCESSION   DASRBV010000254 DASRBV010000000
VERSION     DASRBV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166326"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFA02_09335"
     CDS             <1..366
                     /locus_tag="VFA02_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10096.1"
                     /translation="SVKLSTANYNRTRDYLLGKASSAYIFDPNAFTAAPDFTLGNAKR
                     NYLELRNPAFYDEAANVRKHFYFGERFQGILQVDYFNLLNRTHFSGPDTNISDGTFGQ
                     AQSANSGNRQGQVMFRIEF"
     gene            563..1684
                     /locus_tag="VFA02_09340"
     CDS             563..1684
                     /locus_tag="VFA02_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10097.1"
                     /translation="MAATFACAQSADDEVTPQVQALYEQAKAAQQRGDNTTAIEKYRA
                     MLKLAPHLAPAYNNLGMLYFNQGDYTRAAQALEQGLKLNPDMPTASALLGLSYAQMGE
                     DEKAEPLLEAAVRANPNDDTAQMAFARTLVNLKRYDEATPYLRSYLDRHPKDQQAWYL
                     LGKTYLQLSEDALGRINQIDPNSAMAHIVAGEIDESMKNYDGALVEYKKAIDLAPKQP
                     GMHEHMGNVFWVTGKWESAQREFRSELAINPNNCAVRWKLANAMLEANAPAQDALSEV
                     NEAIERCPGLMQARVDRARALIKLSKPDEALPDLLMAEKDSPDEPSIHFLLSSVYKAQ
                     GKTAEAQQEMRTYGRLQRQASEAVAQQASDAISIKSTAH"
     gene            1761..3500
                     /locus_tag="VFA02_09345"
     CDS             1761..3500
                     /locus_tag="VFA02_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU10098.1"
                     /translation="MTSQQGGAATGGVYAPVHDAEHRPITAGGFVDRGPVVFRDVTQE
                     SGLSSWRHVVGSPEKKYIVESLGSGVALLDYDNDGWVDIYLVNGSTYDAMSGKATPPH
                     AALFHNNHDGTFTDVAEKAGVTNERWGVGVAAGDYDNDGWPDLYVTNLGKNRLYHNNH
                     DGTFTDVAEKAGVALGTWSTSPTFGDYDGDGRLDLFVPGYTKFDFSNPPVPGATSVVS
                     NTCQYRGVVVFCGPRGLKGEQDHLFHNNGDGTFTDVSVKAGVSDPPGYYGLVSLFVDL
                     NGDGRPDLLVANDSTPNYLYRNKGDGTFEDLSYESGYAVNHDGRETASMGIAVGDYLN
                     NGRLDILNTTFSDDYKVLYRNNGEMDFTDVSYDAGIATATVPFLSWGDGFIDYDNDGW
                     KDIFIASGHVYPQADQHDWGTSVAERPLLFRNLGNGKFELVPPVKGTGLADVVRGRGA
                     AFGDLFNDGKTDVVINNVDGPPTLLRNVSDDHHHWIECRLIGGPKSPRDATGAVAFLR
                     TGKIIQREDVLSGGSFASSNDQRVHFGLGNADKIDDLEIRWPDGARENLHPPYVDHIY
                     TIEEGKGILKEQQ"
     gene            3497..5107
                     /locus_tag="VFA02_09350"
     CDS             3497..5107
                     /locus_tag="VFA02_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10099.1"
                     /translation="MTRFVSVWCFLLALAAMSVAQETATPVREADAACARCHQDIYAK
                     YLQTPMANASGRAMDKAIPGVFDHKTSGTEYSISVQNRNLMLRAGESGAERRLDYYLG
                     SGHLGVTYLYSVNGYLFESPIAWYAASHSYDMKPGLGSVTAMPPALPMQASCLRCHMS
                     AVKPSDPGTINHYTGLAFAHTGITCEACHGDTRQHVTSDGKAPVIHLAKLDATRRDSV
                     CISCHLEGDVSVEKAGRSSLDYKPGDNISDYLSYFVYRGKNLTERGVSEVEQLSMSMC
                     KRASGDKMSCTSCHDPHSRPAPEEKAAFYRKKCLACHNDAAFVSSHHPEDPDCTGCHM
                     PRSGAENIPHVAWTDHRIRKVPDKAVMKDLGEGQDLVPVFSPNANQRDLAMAYFRAAM
                     EGNSAFHMKAYRLLEEIRSQLSEDNEALTALALESERQGDYKQAAAIFQQILRRDPQN
                     LTALSNLGTLQAKSGDLQSAATFLNKAFERNEDIVGLAKNLAQVQCMMGDAASAKATL
                     QKTAQYSPGLQDVQQMLERLQTCSGAKP"
     gene            5123..6049
                     /locus_tag="VFA02_09355"
     CDS             5123..6049
                     /locus_tag="VFA02_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10100.1"
                     /translation="MIRSLRYGVFLIGSSALAYSQADPLVSAHSLLEQKQYTQAENLL
                     RGHLATHPTSAAAHFLLGYALFREDRAKESLAEFTEGAKYGSPKPEDLKIVAADYVLL
                     GDFTDADKWLTYVTKEAPEDADAWYLLGRTKYNENRFQEAIDSFQKTLALRPHDIKAE
                     NNLGLCYQGLNRPDEAKKAYENALAWQRDSPVKDAQPFLNAGILLLEQDQSSQALPYL
                     EKAAALAPHNPKVLEQLGRAYDLLGMQEKAERALETAVALAPNASGLHYKLGQVYRRE
                     GKDQLAQQQFDICARLNSTHSSIETPNPAQQE"
     gene            6231..7562
                     /locus_tag="VFA02_09360"
     CDS             6231..7562
                     /locus_tag="VFA02_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZU10101.1"
                     /translation="MTKNLMPGGRPMAIEVSRWLLMKISGKKLLASATVAISFFLSGT
                     ACHAQTHWIGSWAASQQLVEPQNSLAPQDIQDITLRQIIHLSIGGSEVRLRLSNRFGR
                     TPLHLVSVHIALSASRASDKIVPASDKAVTFSGAPDVTIPAGAEYVSDTVIFPVAPLS
                     DVAITLHTDTPPAEQTGHPGSRATSYFVHGDKVSATELPDAKTVDHWYYIAGLDVAAP
                     PESYAIAVLGDSITDGHGATTNGNDRWTDVLAKRLQANVHTSGIAVLNQGIGGNRLLL
                     DGLGPNALARFDHDVIAQPGVRWLIVLEGINDIGTLTRDGDVPAAAHELLVRRIIAAY
                     QQIIMRAHAHNIKVIGATILPFAESGYYHPGPASEADRQAVNTWIRTPGHFDAVIDFD
                     TVVRDPAHPDHLLAAFDSGDHLHPSPAGYAAMANSVPVGLFSADGSNTNKH"
     gene            complement(7578..9368)
                     /locus_tag="VFA02_09365"
     CDS             complement(7578..9368)
                     /locus_tag="VFA02_09365"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZU10102.1"
                     /translation="MGVSFLSFAELCQALAETTKKLEKRALISDYLKPLAAEDAGRAA
                     LYLSGQPFAEADHRVLNVGGSLLSKAIAQLSRASQNAMYTAYRRHGDIGGAAQDLLAT
                     QEGLPPSLTLEDVERSFAGIASARSTAVRLQLVLELLGRATPLQAKYLIKLMTGDMRI
                     GVKQGLVEEAIAHAYGAELAQVRRAVMLTGDLPEVTAMALSGTLAQAKMRLFHPLGFM
                     LASPVASVEEALERFAQEVAAEHGEAEAKPQQALHEAQLEDKYDGVRAQLHCGDTGQP
                     GRVALFSRNREDISESFPELVEAFAQISEPAILDGEIVAWTPADSRALPFTSLQQRLG
                     RKRVSREWRDRVPVVFIAFDLLFLAGELQLERTLRERRQSLEAFVARISEATARLEQR
                     GQAALFGEATDAQHYPHIVLAPAVQLRSAEQLDRSYAEARERGNEGVMIKALNSTYQP
                     GRRGLAWLKLKRELATLDVVVTGAEYGHGKRAGVLSDYTFAVRDGDKLKNIGKAYSGL
                     TDAEINELTRFFKEHTMEDYGHFRTVEPLIVLEVAFNNVMRSDRHNSGFALRFPRILR
                     IRNDKPVDEIDTLQRVEEIYNAQPDKAVEP"
     gene            9577..10653
                     /gene="prfA"
                     /locus_tag="VFA02_09370"
     CDS             9577..10653
                     /gene="prfA"
                     /locus_tag="VFA02_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZU10103.1"
                     /translation="MFERLDQIAARYEELGEQLASPEVVSDPQKYQKIAKQHRELEAV
                     VEKYREYKQVQTGISDAKVMLNESDPDIRAMAGEELAVLEERLPKIEEELKLLLLPKD
                     PNDEKNVILEIRAGTGGDEASLFAAEIFRMYSRFAEQKRWRVEVLSSSESSVGGLKEV
                     IAIIEGDHVYSQLKYESGVHRVQRVPATETQGRVHTSAITVAVLPEAEDVDIKIEAKD
                     IRIDTFCSSGPGGQSVNTTYSAVRITHLPTNTVVSCQDEKSQIKNREKAMRVLRARLY
                     EVEMEKQQEALAKERKQQVGSGDRSEKIRTYNFPQNRLTDHRIGLTIHQLADVMEGKL
                     QPVIDALIAHFNAERLKAETASAA"
     gene            complement(10672..>12090)
                     /locus_tag="VFA02_09375"
     CDS             complement(10672..>12090)
                     /locus_tag="VFA02_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZU10104.1"
                     /translation="VFFLAAVIPYLAITGEWRKWRQLRPVTGLLLFLAIAAPWHILAG
                     IRNPDHGNPIGNVPTPGHVHGFFYFYFINEHVLRFLGKRYPHDYNKQPFWVFWTGQLV
                     WLFPWSLYFPVILRRAWRNRRLAWADVRYDASNTLHFLDPKSTAYEAASMAARVRFRA
                     RTGLLLVIYAAFILVFFSISTNQEYYTWPAYFPLLVLTAGALASIEEAPEAGRWINNR
                     SKWLTGAHAAFAIVGTAAAVALGIGLWTSRNLPYVPDIGTLLAHRGVGDYSLATSHLF
                     DLTGPSFAALRLPAGLAAIALLAGPIIAWFLRRRGNAFEATVSVGFTAAVFLIAAHIA
                     FVRFGPMLSSRAMADTINRLGSPGDELLIYGDQADGSSVIFYTQKQALLVHGTNSYFE
                     PDSNHPGQTFGSSMIWGANYPDAPHIFLSDKDLLAMWGKGPRKFLFVPGDYTAHVEQL
                     LQGRLYKLQELSDKTLYTDRPL"
BASE COUNT         2849 a         3427 c         3359 g         2455 t
ORIGIN      
        1 tcggtcaagc tgagcaccgc gaactacaat cgcacgcgtg attacctttt gggaaaggca
       61 tcttcagcct atatctttga cccaaatgct ttcacagcag ctccagactt tacactcggc
      121 aatgcaaagc gtaactatct cgaactgcgc aacccagcgt tctacgatga agctgcaaat
      181 gtccgtaaac atttctactt tggagaacgg ttccagggca ttcttcaggt ggactacttt
      241 aacttattaa accggaccca tttcagtggc ccagacacaa acatcagtga tgggaccttt
      301 ggacaagccc agtcagcaaa tagcggcaac cgacaaggcc aggtgatgtt ccgaatagaa
      361 ttctaggcct tagaaacgcc ctactgatga gaacatcaga ggggcaaagg tttccaacga
      421 atgtaacact tgcctccatg ccggactgct gaccacagtc cggcattttt cttcattgcg
      481 aatgctgaat caagtcagaa ataatggcgc tgcgatgatt ccccgcaaat ctgttgtcat
      541 ttggttttcc tgttgcctgc ttttggccgc cacctttgcc tgtgcgcaat ctgccgacga
      601 cgaggtgacg ccgcaggtac aggcgctcta tgagcaggcg aaagcggcgc agcagcgggg
      661 agacaacaca accgccatcg agaagtaccg cgcgatgctc aagctcgctc ctcaccttgc
      721 gcccgcgtac aacaatctgg ggatgctgta ctttaaccag ggagactaca cgcgcgcagc
      781 acaggcgctg gaacaggggc tgaagctgaa tcctgatatg ccgacggcct ctgcgctgct
      841 gggcctgagc tatgcgcaga tgggcgagga cgagaaggcc gaaccgctgc tggaggctgc
      901 cgtccgcgcc aatcccaatg atgacactgc acagatggcc tttgcccgca cacttgtcaa
      961 tctgaagcga tacgacgagg ccacgccgta tctgcgcagc tatcttgacc ggcacccgaa
     1021 agaccagcag gcatggtatc tgctgggcaa gacttacctt cagctttcag aagatgctct
     1081 tgggcgcatc aaccagattg atcccaactc tgcgatggcg cacatcgtag cgggcgagat
     1141 tgacgagagc atgaagaatt atgacggcgc actggtggaa tacaagaagg ccattgatct
     1201 ggcaccgaag cagccgggca tgcatgagca tatgggtaat gtcttctggg taacgggcaa
     1261 gtgggagtcg gcgcagagag agtttcgctc cgagctggcc atcaatccca acaactgcgc
     1321 cgtgcgctgg aagctggcca atgcgatgct ggaagcaaat gctccagcgc aggacgcgct
     1381 gagcgaagtg aacgaggcca ttgagcgctg tcccggcctg atgcaggccc gcgtagaccg
     1441 cgcgcgcgcg ctgatcaagc tgagtaaacc ggacgaggcc ctccctgatc tgctgatggc
     1501 cgaaaaagac tctcccgatg agccttcgat ccattttctt ttgtcttctg tgtacaaggc
     1561 gcagggcaaa actgccgaag cgcagcagga gatgcgcaca tatgggcgcc tgcaacggca
     1621 ggcgagtgag gctgtggcgc aacaggcgag cgatgctatt tcgatcaaaa gcacagcgca
     1681 ttgaattttt catcatgctc cgggtaatcc accttgccgc attttcctgt ctgctttcgc
     1741 cagtgctcct ctttgcccag atgacctcgc agcagggagg cgctgctacc ggaggcgtct
     1801 acgctccggt gcatgatgcc gagcaccggc ccattaccgc tggaggattc gtggaccgtg
     1861 ggccggtcgt cttcagagat gtaacgcagg agtcgggcct ttcatcctgg cggcatgtgg
     1921 ttgggagccc ggaaaagaaa tacattgtcg agtcgctcgg cagcggcgtg gccctgctcg
     1981 actatgacaa cgatggatgg gtggacatct atctggtcaa cggctcgacc tatgatgcga
     2041 tgagtggaaa ggcgactccg ccccatgccg cgctttttca caataatcac gacgggacat
     2101 ttactgatgt cgcagaaaaa gcgggtgtga cgaatgagcg ctggggtgtg ggcgtggccg
     2161 caggcgacta cgataacgac ggctggccgg atctttacgt caccaacctg ggcaagaacc
     2221 gcctgtacca caacaatcat gacgggacct ttaccgatgt tgcggaaaag gccggagtgg
     2281 cgctgggcac atggtctaca agtcccactt ttggcgatta cgatggcgac ggcagactcg
     2341 accttttcgt tcccggatac acaaagttcg acttcagcaa tccacctgtt cctggtgcaa
     2401 cgtcggtggt ctcgaatacc tgccagtacc gcggagttgt ggtcttctgc ggcccgcgcg
     2461 gattgaaagg cgagcaggac catctcttcc ataacaatgg agacggcaca ttcactgacg
     2521 taagcgtgaa ggcaggcgtc tctgatccgc ctggatacta cggtctcgtc tcgctctttg
     2581 tagacctgaa cggggatggc agacccgatc ttttagttgc gaatgactcc accccgaatt
     2641 atctctaccg caacaagggc gacgggacat ttgaggacct gagctacgaa tcgggttatg
     2701 ctgtgaacca cgatggccgc gaaaccgcaa gcatgggcat tgccgtgggc gactacctca
     2761 acaacggacg cctcgacatc ctgaacacga ctttttctga tgactacaag gtcctctatc
     2821 gcaataacgg tgagatggac tttacagatg taagctatga cgccggaatt gcaacagcca
     2881 cggtcccgtt tctaagctgg ggcgatggtt ttatagatta cgacaatgac ggctggaagg
     2941 acatcttcat cgcgagcggc catgtgtatc cgcaggcaga ccagcacgat tggggcacca
     3001 gtgttgccga gaggccgctg ctcttcagaa atctcggcaa tgggaagttt gagcttgttc
     3061 cacctgtcaa gggaacgggg ctggccgatg tggtccgcgg gcggggcgcg gccttcggcg
     3121 atttattcaa tgacggaaag acagacgtgg tcatcaacaa cgtagacggt cctccgaccc
     3181 tgttgcgaaa cgtcagcgat gaccatcatc actggatcga gtgcaggctg atcggtggcc
     3241 ccaaaagccc gcgcgacgca acaggcgcag tggccttcct caggacagga aaaatcatac
     3301 agcgcgaaga tgtactgagc ggcggcagct tcgcttcatc gaatgatcag cgtgtccact
     3361 ttggcctggg caatgctgac aaaatagatg acctcgaaat ccgctggccc gatggcgcgc
     3421 gggagaatct tcatccaccg tatgtagacc atatttacac aatcgaagaa ggcaaaggca
     3481 ttctgaagga gcagcagtga cgcgattcgt ttctgtctgg tgctttcttc tcgcgcttgc
     3541 cgctatgagc gttgcacagg aaacagcaac acctgtgcgt gaagcggatg ctgcgtgcgc
     3601 gcggtgccat caggacatct acgcaaagta tttacagacg ccgatggcca acgcgagtgg
     3661 ccgtgcaatg gacaaggcca ttcccggcgt gttcgaccac aaaacatcgg gcacggaata
     3721 cagcatctct gtccagaaca ggaatctgat gctgagggca ggagaatccg gcgccgagcg
     3781 caggcttgat tactatctcg gatccggtca cttaggtgtg acgtatcttt attccgtcaa
     3841 cggctactta tttgaatcgc ctatcgcctg gtatgcggct tcgcattcct acgacatgaa
     3901 gcccggcctt ggttccgtga cggcaatgcc gccggcgctt cccatgcagg ccagttgcct
     3961 gcgctgccac atgagcgctg tcaagcccag cgacccgggt acgatcaacc actacacagg
     4021 ccttgcgttc gcccacaccg gaattacctg cgaagcatgc cacggagata cgcggcagca
     4081 tgtgacaagc gacggcaagg ccccggtcat ccatctggca aagctcgacg cgacgcgacg
     4141 tgattcggtc tgcatcagtt gtcatctgga aggcgatgtc tccgttgaaa aagcaggccg
     4201 ttcttcactt gattacaaac ctggagacaa catttcggac tatctttctt actttgtcta
     4261 tcgcggaaaa aacctgaccg agcgcggcgt gagcgaagta gagcagctca gcatgagtat
     4321 gtgcaagcgc gccagcggag acaaaatgtc atgcaccagt tgtcacgatc cgcacagccg
     4381 gcctgcgcca gaggagaaag ctgcctttta cagaaagaaa tgtctcgctt gccacaacga
     4441 tgcggcattt gtctcatcgc atcatcctga ggaccccgac tgcaccggtt gccacatgcc
     4501 ccgcagcggc gcagaaaaca ttcctcacgt tgcatggaca gaccaccgca tccgaaaagt
     4561 tccggacaag gcagttatga aagatctggg tgagggccaa gatctcgtcc cggtcttttc
     4621 gccgaatgca aatcaacgtg accttgcgat ggcatatttc cgcgcagcta tggaaggcaa
     4681 ctccgccttt cacatgaagg cctaccggct tctcgaagaa atcaggtcac agctctcaga
     4741 agacaacgag gcactcaccg ctctggccct tgaaagcgaa cgccaaggag actacaaaca
     4801 ggccgcggcc atcttccagc agatactcag gcgcgatccg caaaatctca cggccctgtc
     4861 caatctgggc acgcttcagg caaaatccgg cgatctgcaa agcgccgcca catttcttaa
     4921 taaggcattc gagcggaacg aagacattgt tggactggca aaaaatctgg cacaggtgca
     4981 atgtatgatg ggcgatgcgg catccgcgaa ggccaccttg cagaaaacgg cgcagtacag
     5041 tccaggtctt caggacgtgc agcagatgct ggagcggctt caaacctgct ccggcgcaaa
     5101 gccctaaaat aaacaggcat ccatgatcag gtcactgcga tacggcgttt ttcttattgg
     5161 gtccagtgcg ctggcatatt cccaggcaga cccgttagta agtgcgcatt cgctcctcga
     5221 gcagaagcaa tatacgcagg cagaaaatct tcttcgcggt catcttgcca cacatcccac
     5281 gtcggctgcg gcccactttc ttctcgggta cgcgctcttc cgggaagacc gcgccaaaga
     5341 atctcttgcc gaatttacag agggtgcaaa atatgggtcc ccgaagccag aggacctgaa
     5401 gattgtcgca gccgattatg ttctgctcgg cgatttcacc gatgcggaca agtggctgac
     5461 gtatgtaaca aaagaagctc cggaagatgc cgacgcgtgg tatctgcttg gccgcaccaa
     5521 atacaacgaa aaccggtttc aggaagcgat tgacagcttt cagaagacgc tggcgcttcg
     5581 cccgcatgac atcaaagcag agaataatct gggactctgt tatcaggggc tgaaccgtcc
     5641 agacgaagca aaaaaggcct atgaaaatgc gctcgcatgg cagagagatt cgccagtaaa
     5701 agatgcgcag ccctttctga atgcaggaat cctgttgctg gagcaggacc agtcttcgca
     5761 ggcgttgcct tatctggaaa aggccgccgc tctcgctccg cacaatccaa aagtgctcga
     5821 acaacttggg cgcgcttacg atttacttgg aatgcaggaa aaggccgagc gagcgcttga
     5881 aacggcagtg gccctcgccc ccaacgcctc tggtttgcac tacaaactcg gacaggtcta
     5941 tcgccgcgaa ggcaaagacc aactggcgca gcagcagttt gatatctgcg cgagattgaa
     6001 cagcactcac tcctccattg aaacgcccaa tcccgcccag caggagtagt tattcgttgc
     6061 cagtccccgg ttgattcgtt attagtcccc agttcggttg ctaaacttta catcgctgag
     6121 attcttcctc cctgcggtcg tcagaatgac ggactctact ttacaaggaa tgctctgaaa
     6181 atcaacctct ggctgctgta cattgtttga atagaaaact aatagaacct gtgacaaaaa
     6241 acctgatgcc tggaggccgt cctatggcga ttgaagtctc tcgttggctg ttgatgaaaa
     6301 tttccggcaa aaaacttctg gcttccgcta ccgtcgctat ttccttcttt ctttcaggga
     6361 ctgcatgcca tgcgcagacc cactggatag ggtcctgggc agcttcgcag caactggtcg
     6421 aaccgcaaaa ttcgcttgct ccccaggaca ttcaggacat aactctgcgc cagatcatcc
     6481 atctctccat tggcggaagt gaggtccgtc ttcgcctctc caaccgcttt ggcagaacgc
     6541 cgcttcatct ggtctcagtc cacatcgcgc tttccgcttc gcgcgcttca gacaaaatcg
     6601 tgcctgcaag cgacaaggcc gtcacctttt ccggagcgcc tgatgttacc attcccgcag
     6661 gcgcagaata tgtatccgat acggtgatat ttcccgttgc gcctttgtca gacgtagcga
     6721 taaccctgca tactgacaca cctccggcgg agcagaccgg ccatcccggc tcgcgcgcca
     6781 cttcatattt cgtgcatggg gacaaagttt ccgccaccga actgcctgat gcaaagactg
     6841 tagaccactg gtactacatc gcagggctgg atgtggcagc gccgcctgaa tcctatgcca
     6901 ttgcagtgct tggcgattcc attaccgatg gacacggtgc gactaccaat ggcaatgacc
     6961 gctggacgga cgtgctggca aagaggctgc aggccaatgt tcatacttcc ggcatagctg
     7021 ttctcaacca gggaatcggt ggaaaccgtc tgctcctcga tgggcttggc ccgaacgcgc
     7081 tggcacgctt cgatcacgac gtaattgccc agccgggagt gcgctggctc atcgtacttg
     7141 aaggcatcaa tgacattggc acgctcacgc gcgatgggga tgttcctgcc gctgcgcacg
     7201 aactgttggt ccgccgcatc atcgctgcat accagcagat catcatgcgc gcccacgcgc
     7261 acaacatcaa agtgattggc gcgacaattt taccttttgc ggaatcaggc tattatcatc
     7321 cggggccagc gagcgaagca gaccgtcaag ccgtaaatac atggatacga acaccgggcc
     7381 attttgatgc agtcattgat tttgatacag tggtgcgcga cccggcgcat ccagaccacc
     7441 tgcttgctgc ctttgactcc ggcgaccatc tgcacccttc gcctgccgga tatgctgcca
     7501 tggccaacag cgttccggtg gggttgtttt ctgcggatgg aagcaacaca aacaaacatt
     7561 gagaaaagca gcaccgctca tggctccact gctttgtccg gctgcgcgtt gtaaatctct
     7621 tctacgcgct gcagcgtgtc aatctcatcc accggtttgt cattgcggat acgcagaatg
     7681 cgcggaaaac gcagcgcaaa gccgctgtta tggcggtctg agcgcatgac attgttaaaa
     7741 gcaacttcaa ggacaatcaa cggttccacc gtgcggaagt gtccgtaatc ttccattgtg
     7801 tgctctttga agaaccgggt cagttcgttt atttcagcat cggtcaagcc agaataggcc
     7861 ttgccgatgt tcttcagctt gtcgccatcg cgaacggcaa acgtgtagtc gcttaaaacg
     7921 cccgcacgct tgccatgtcc gtattcggcg ccggtcacaa caacatccag tgtcgccaac
     7981 tcgcgcttca gtttcagcca tgccagccca cgccggcccg gctgatatgt gctgttcagc
     8041 gccttgatca tgacgccttc attgccgcgc tcgcgcgctt cagcatagga gcggtcgagc
     8101 tgctctgcgc tacgcaattg cactgccgga gcaagcacga tgtgcggata atgctgcgca
     8161 tctgtggctt caccgaagag cgctgcctga ccgcgctgct ccaagcgcgc cgttgcctct
     8221 gaaatgcggg ccacgaaagc ttccagcgat tgcctgcgtt cacgcagcgt ccgttccagt
     8281 tgcagttcac ccgcaagaaa gagcaaatca aatgcgataa acacaaccgg tacgcggtcg
     8341 cgccattcgc gggaaacgcg cttgcgccct agccgctgct gtaatgatgt aaaaggcagc
     8401 gcgcggctgt ccgccggggt ccaggcgact atctctccat ccaggatggc cggttcggag
     8461 atttgcgcga aggcttcgac aagctcagga aagctctcgc tgatgtcctc acgattgcgc
     8521 gaaaaaaggg cgacacgccc gggctgcccc gtatctccgc aatgcagttg cgcgcggacg
     8581 ccgtcgtact tgtcctcaag ctgtgcctcg tggagtgctt gctgaggctt cgcttctgcc
     8641 tcgccatgct cagccgcaac ttcctgcgca aaccgctcca gtgcttcttc cacggatgcg
     8701 acgggactgg ccagcatgaa ccccagggga tgaaagagcc gcatttttgc ctgcgccaac
     8761 gtgccagaga gcgccatggc cgtaacttcc ggaaggtccc cggtcagcat cacggcacgt
     8821 cgcacctgcg cgagctccgc gccataggca tgggcgatgg cttcttccac caggccctgt
     8881 ttcacgccga tgcgcatgtc tccagtcatg agcttgatga ggtactttgc ctgaagaggc
     8941 gtggcgcggc ccaataattc aagcacgagc tgcagccgga ccgcagtact gcgcgctgag
     9001 gcgatcccgg caaaactgcg ctccacgtct tccagggtga gcgatggtgg gaggccctcc
     9061 tgcgttgcga gcaggtcctg cgcggcaccg cctatatcgc catgtctgcg gtaagcggta
     9121 tacatcgcgt tctggcttgc gcgggaaagc tgtgcgattg cctttgaaag caaggacccg
     9181 cccacattga gcacgcggtg gtcggcctcg gcaaaaggct gcccggaaag gtaaagagcg
     9241 gcccgccctg catcttcggc agcaagcggc ttcaggtagt ccgaaatgag ggcgcgcttt
     9301 tcgagcttct tcgtggtttc agccagcgcc tggcacaatt cagcgaaaga caggaagctt
     9361 acgcccatgt ctgttgaata tagagcagaa gcatggcagg atgcgttctg tgaacggaga
     9421 ggcccgagga atatgaatcc gctgtacaat agaggagatg cagtttgtct ttacaaacgg
     9481 cgctcttctt tagcggccct ggccgccgat acactcgtct gcttattttc ccaaacaatc
     9541 aaggtcaact gacagagcgc aaaagagaga atttccatgt ttgaacgttt agaccagatt
     9601 gccgcccgct atgaagagct tggcgagcag cttgcttcgc ccgaggttgt atcggacccg
     9661 cagaagtacc agaaaattgc caaacaacac cgtgagcttg aggccgttgt agaaaaatac
     9721 cgcgaataca agcaggtgca gaccggcatc agcgacgcca aagtaatgct caacgagagt
     9781 gacccggaca ttcgcgccat ggccggcgag gaactggccg tcctcgaaga gcgtctgccg
     9841 aagatagaag aagagctgaa gctgctgctg ctgcccaagg acccgaatga cgaaaagaac
     9901 gtgatcctcg aaatccgcgc aggcacgggt ggtgacgagg catctctgtt tgcggctgaa
     9961 atcttccgca tgtactcgcg ttttgccgaa cagaagcgct ggagggtgga agtactttcg
    10021 agttcagagt cgtccgtggg tggactgaag gaagtcatcg ccatcattga aggcgaccac
    10081 gtttattcac agttgaagta tgagagcgga gtccaccggg tgcagcgcgt tcctgcaaca
    10141 gaaacgcagg gccgtgtgca tacgtcggcc ataactgtcg ccgtgctgcc ggaagccgaa
    10201 gacgtggaca tcaagattga agccaaagac attcgcattg acaccttctg ctcgtccggg
    10261 ccgggcggac aatccgtaaa cacgacttat tcggcagtcc gcatcacaca cctgcccacg
    10321 aatacggtcg tgagctgcca ggatgaaaag tcgcagatta agaaccgtga aaaagccatg
    10381 cgcgttctgc gcgcccggct gtatgaagtt gagatggaaa aacaacagga ggcgctcgca
    10441 aaagagcgca aacagcaggt cggttccggc gacagaagcg agaagatccg tacctataac
    10501 tttccgcaga accgcctcac ggaccatcgc atcgggctga cgatccacca gttggcagat
    10561 gtaatggaag gcaagctgca gcctgtgatt gatgctctca tcgcccactt caacgcagag
    10621 cggctgaaag cagaaacagc ttccgccgcc tgaaatgagc gatggaaaag ctcacagtgg
    10681 cctgtccgta tagagcgtct tgtccgacag ctcctgcaat ttataaagcc gcccctggag
    10741 aagctgttct acatgggcgg tataatctcc gggcacaaag agaaatttgc gcggcccttt
    10801 tccccacatc gccagcaggt ctttatcact tagaaagatg tgcggagcat ccggataatt
    10861 tgctccccag atcatcgagc tgccaaacgt ctgtccagga tgattgctgt cgggttcgaa
    10921 gtaagaattc gtgccgtgca ccagcagcgc ctgtttctgc gtatagaaaa tgacggacga
    10981 tccatcggcc tggtcgccat aaatcaataa ctcatcgcca ggtgagccaa gacggttgat
    11041 tgtgtctgcc atggcgcgcg aagagagcat aggtccaaag cgcacgaaag cgatatgcgc
    11101 tgcgatgaga aagaccgccg cggtaaaccc gacagacacg gtggcctcaa atgcgtttcc
    11161 gcgccggcgc agaaaccacg caatgatggg gccagccagc aacgcgatgg cggccagccc
    11221 ggcaggcaga cgcagggcgg caaatgatgg tccagtaagg tcaaacaagt gcgacgtggc
    11281 cagcgaataa tcacccacgc cgcgatgggc cagcaatgtt ccgatgtccg gcacatacgg
    11341 cagattgcga ctcgtccaaa ggccgatacc aagcgcgacc gccgcggccg taccgacaat
    11401 ggcaaaggcc gcgtgcgctc cggtaagcca cttgctgcga ttgttgatcc agcggccggc
    11461 ttcaggggct tcttcaatgg aagcaagagc gcccgccgtc agcaccagta gtgggaaata
    11521 cgcaggccat gtgtaatatt cctggtttgt ggaaatggaa aagaagacca gaatgaacgc
    11581 agcatagatg acgagcagca atcccgtgcg cgcacgaaag cgcactcgcg ccgccatgct
    11641 tgcggcttca taggcagtgc tcttcgggtc gagaaaatgc agtgtattcg aagcgtcgta
    11701 acgcacatcg gcccaggcaa gtctgcggtt gcgccacgcg cggcggagga ttaccggaaa
    11761 atacaggctc cagggaaaga gccacaccaa ttgcccggtc cagaagaccc agaagggctg
    11821 cttgttgtag tcatgcggat agcgcttgcc cagaaaacgc agtacgtgct cattgatgaa
    11881 gtagaagtaa aagaacccat gtacgtggcc cggcgtggga acattgccaa ttgggttgcc
    11941 atggtccgga ttgcggatcc ccgccagaat gtgccatggc gcggcgatcg ccaggaacaa
    12001 cagcagaccc gtcaccgggc gaagctgccg ccacttgcgc cactcgccgg taatcgcaag
    12061 ataagggatg accgctgcaa gaaagaacac
//
LOCUS       DASRBV010000255         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_71126,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000255 DASRBV010000000
VERSION     DASRBV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71126"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1312
                     /locus_tag="VFA02_09380"
     CDS             149..1312
                     /locus_tag="VFA02_09380"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HZU10105.1"
                     /translation="MTRSISRRGFIKGAAFASLAAKAGLAQGKTIQEKQILFVGTQTG
                     PASKGIYAFEWDAARGLLKELGLAAESDNPTFLALSPDNKRLYAANEINKYQGATSGA
                     ISGFTVDRNAARLTLINTVPSTASGPCHVAVDATGHTVLCANYSGGGAASFHADAQGR
                     LSQAVSEFHYHGHGVVADRQEAPHAHRATVSPDNRFVYINDLGLDCIHIYRLDAATSK
                     LAPNAPAQWTAKPGSGPRALRFHPNGKWAFCIHELACALEVLEWNAHIGALTSLHRVN
                     LLPADFHQPSLASEIVFDREGKFAYAAVRGYDHVMTYAIDPTSGALTLLDRTSAGGRT
                     PRHIALDPADGWLLVANQDSDNIAVIRRDRQTGRLEKTGESFPQIKPQCLVFV"
     gene            1375..2127
                     /locus_tag="VFA02_09385"
     CDS             1375..2127
                     /locus_tag="VFA02_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10106.1"
                     /translation="MLKFPFVVFLVSLVFLWLSVQMGSVLAARFRPVKENERDDLNLV
                     ISASLTLLALIIGFSFSMAVSRYDQRKNYEEEEANAIGTEYVRAGLLPAADAVRIRQL
                     LSRYLDQRILFYTVRDASRLKTVNTETAKLEDEMWSAVQSAAAAQPTPTVALAVAGMN
                     DVLNRQGYTQAASWNRIPVGAWTLMSTLAICCCLLIGYGAHRRGALLFMVFPFLVAIA
                     FFLIADIDSPRQGVIRVAPQNLISLSQSLHAH"
     gene            complement(2144..2683)
                     /locus_tag="VFA02_09390"
     CDS             complement(2144..2683)
                     /locus_tag="VFA02_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10107.1"
                     /translation="MSTQPAVEDISAVVSRFQAWAGTQTQSNPEVREISYEEALHPKR
                     KKAPAKKRCTQPKKPAPVKQKEPAFRQVLAEKTAILPAAKKTTAALATRTTTLSVRIT
                     DAEQKLLKVRAAEAGLSASAYLRQCILDVDDLREQVQELLAENQTLRAAASSGILSGL
                     AQFMRRIFGGNTTALSVRA"
     gene            complement(2844..5657)
                     /locus_tag="VFA02_09395"
     CDS             complement(2844..5657)
                     /locus_tag="VFA02_09395"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZU10108.1"
                     /translation="MPLLWKPESWPQRLAELQARSGDLKEVPLRRDVRSLGMLLGEVL
                     REQAGEELYEKVEELRTTAIRRREAQAAGNASEAEKLMQRSVRSIAEVSVERAYQLSR
                     AFAFYFELINLAETNHRKRRRLSLQLSGGSEVQRGSLRGTLQEMKRVGITANEALEWL
                     RRIFIVPVFTAHPTEVARRSVLFKRRRIGEFLEQLDRVPIPDEEIARLEEALLAEITA
                     LWQTDEVRSRRPTVNDEIKMGLDYYDVSIFATLPKLYEEVSAALASVYGIEIDISELP
                     LLLGFGSWIGGDRDGNPFVTPEVTLDAIRAARTRLLAYYEKQVQLIIDLLTTSAQQLP
                     ISEELSQRLHRYLSELRTGTQQVFGARFEFEMYRRFMVCVHARLLRTAGKEASDTDSL
                     EAALHALPAYCNAQEFLDDLRVLRRSLAENQGLRLARTLIDPLILLVRTFGLHLHTLD
                     IRQHVNLHQKTLKEASAWCAGDAASVPQNLSPESARVIETFRAVAEVKRGCSPEAIRQ
                     YVISGASTVEDILGVLWLARLGGVECAGKNGDPGLMPVPLFEYIGDLRNAHEVCRRLW
                     NIPEYRNLLASWNNTQEIMLGYSDSNKDGGMLTSTWEIFRAHRALHEVARECGVKLRL
                     FHGRGGTVGRGGGPTHRSIYAQPVGAFEGQIRITEQGEVLNWKYSDVVLAERNLELMI
                     AASLDALARPNALDPNGHQSGVLLPEWEKAFDELSETAFQFYSQQIVGDPEVFQYFEL
                     ATPVGELEHARIGSRPTRRSGKRSFADLRAIPWVFGWTQSRLLVPGWFGVGHAIEQYT
                     KKHSNGLKLLRDMMAEFPLFIDLIRNVEMAMAKADLGIASLYASLVPDAAMRQRVFSK
                     LEAEFERTLKAVLAITEQKELLENNQVLARSIRLRNPYVDPMSLIQVDLLRRKRAGED
                     TDEINRAIAGTINGISAGLRNTG"
BASE COUNT         1182 a         1693 c         1688 g         1249 t
ORIGIN      
        1 cgcaacaatc cgcagacact caagcgtatc cgcggccagg tgctcgagct ggctgagcag
       61 ttcccgctgt atggatggct gcgccaacca gcggttgtgg catagttcgc cttcaaaatg
      121 acatccatat agcatgagga ccatctgcgt gactcgttct atctcccggc gcggcttcat
      181 caaaggagct gcgtttgcat cgttagcagc caaggccgga ctggcacaag gaaagaccat
      241 tcaggaaaaa cagattctct ttgtcggcac gcagacaggc cctgcaagta agggtattta
      301 cgcctttgag tgggacgcgg cccgtggctt gctgaaggaa cttggtctcg cagcggaaag
      361 tgacaatccg acatttcttg ctttatcgcc ggacaacaag cgtctttatg cggcgaacga
      421 aatcaacaaa tatcagggcg caacgagcgg agcaatcagc ggatttacgg tggaccggaa
      481 cgccgcaagg ctcacgctga tcaataccgt tccttccacg gcatccggtc cgtgccatgt
      541 ggcggtggat gcaaccgggc acacagtcct ttgcgcaaac tactccggag gaggcgcggc
      601 ctcattccat gcagatgcgc aggggcggtt gagtcaggcc gtttcagaat ttcactacca
      661 cggacatggc gttgtggccg atcgccagga agctccacat gcgcatcgag ctaccgtctc
      721 gccggacaac cgctttgtct atatcaacga cctcggcctg gattgcatcc atatctaccg
      781 cctcgatgcg gcgacctcga agctggcgcc gaacgctcct gcccagtgga cggcgaagcc
      841 cggctccggt ccgcgggcac tgcgctttca tcccaatggc aaatgggcct tctgcatcca
      901 tgagctggcc tgcgcgctgg aagtgctcga atggaatgct cacatcggcg cgcttacgtc
      961 actgcatcgc gtgaatctgc tgcccgcgga tttccaccag ccatcgcttg cttcggagat
     1021 tgtcttcgac cgcgaaggaa agtttgccta tgcggctgtc cgcggatatg accacgtcat
     1081 gacttatgcc attgatccga catcaggcgc attgacgttg ctcgaccgca cttcagccgg
     1141 aggccggacg ccgcgccaca tcgcgctcga tcctgcagat ggctggcttc tcgtagccaa
     1201 ccaggattcc gacaacatcg ccgtcattcg cagagacagg cagactggca ggctggagaa
     1261 gacaggggag agctttccac agataaagcc gcagtgcctg gtgtttgtct aaaatgaagc
     1321 tgcccgtcat tcctcccgga tggacctgga aaggagcacg cagctttggg cgaaatgctg
     1381 aagttcccgt tcgtggtttt tctggtctcc cttgtcttcc tttggctctc ggtgcagatg
     1441 ggcagtgtcc tggccgccag attccgtccg gtgaaagaga atgagagaga cgatctcaac
     1501 ctcgtcataa gtgcaagcct cacactgctg gccctcatca taggattcag tttttccatg
     1561 gccgtgagcc ggtacgatca gcgcaaaaat tatgaggagg aagaggcaaa cgcaatcggc
     1621 acggagtatg ttcgcgccgg tctgctgccg gcggccgatg ccgtaagaat tcgccagttg
     1681 ctctcgcggt atcttgatca gcgcatcttg ttttataccg ttcgcgatgc ctccaggctc
     1741 aaaacggtca acactgagac ggcaaagctc gaggatgaga tgtggtcggc cgtacagagc
     1801 gctgcggccg cacagcccac ccctacagtg gccctggctg ttgcgggaat gaacgacgta
     1861 ctcaaccggc agggatacac acaggctgct tcctggaacc gcattcccgt gggagcgtgg
     1921 accctgatga gcactcttgc aatttgctgt tgcctgctga ttggctatgg cgcacatcga
     1981 agaggcgctt tgctgttcat ggtctttccg ttccttgtgg cgattgcatt tttcctcatc
     2041 gccgacatag acagcccgcg ccaaggagtc atccgcgtgg cgccgcaaaa tctgatcagc
     2101 ctgtctcagt cattgcacgc acactaagcc attttcatag attctaggcc cggacggaca
     2161 gcgcggtcgt gtttccgcca aagatgcggc gcatgaactg ggccaatcca gacaatatgc
     2221 cgctggatgc cgccgcccga agcgtctggt tttcggccag cagctcctgt acctgttcac
     2281 gcaaatcatc cacgtccaga atgcactggc gcagataggc agaggcggaa aggccggctt
     2341 ctgccgcgcg gaccttcagc agcttctgtt cggcgtctgt aatgcgcacg gacagcgttg
     2401 ttgtccgggt tgccagtgct gctgtggtct tttttgccgc aggaaggatg gctgtctttt
     2461 ccgccagcac ttgcctgaat gccggttctt tctgctttac tggcgcgggt ttcttcggct
     2521 gggtacagcg ctttttcgca ggcgcttttt tccgcttcgg gtgcagcgcc tcttcgtagg
     2581 aaatctcccg gacctcagga ttactttgtg tctgcgtacc cgcccacgcc tgaaaacggc
     2641 ttacgacggc gctgatgtct tccactgccg gttgcgtgct catgtgctga gtgtatgccg
     2701 tgggaaaggg gacggtagat tctccgaaga aaaatactgt ccccatattt tcagaatgga
     2761 actgctctgc gaacagttcc atttcaggaa aaagcagggc cttcattcac agaagggctg
     2821 aacgaatgct tccggtttat caatcatcca gtgttgcgca gccctgcgga gatgccgtta
     2881 atggtcccgg caatggcgcg gttgatctcg tccgtatctt cgcccgcgcg tttgcggcgc
     2941 agcaggtcca cctggatgag gctcattggg tccacatacg gattgcgcag gcgaatggag
     3001 cgggccagca cctgattgtt ttccagcagt tctttctgtt ccgtaatggc gagcactgcc
     3061 ttcagcgtgc gctcaaattc agcttcgagc tttgagaaga cgcgctgacg catcgctgca
     3121 tccggcacaa gcgaggcata aagcgaagcg atgccgaggt cggccttcgc catcgccatt
     3181 tcgacgttgc ggatgaggtc aataaaaagc ggaaactcgg ccatcatgtc acgcagcagc
     3241 ttcaggccgt tgctgtgctt tttggtgtac tgctcaatgg catggccgac gccgaaccat
     3301 cctgggacca gcagacggct ctgcgtccat ccaaaaaccc acgggatggc gcgcaggtcg
     3361 gcaaagctgc gctttccgct gcggcgtgtg ggacgggagc cgatacgcgc gtgttccaac
     3421 tcgcctacgg gagtggccag ctcgaaatat tgaaagacct caggatcacc gacgatctgc
     3481 tggctataga actgaaaggc cgtctcagac agctcatcga aggccttttc ccactccggc
     3541 agcagcacgc cggactgatg tccgttcgga tcaagcgcat tggggcgcgc cagtgcatcg
     3601 agtgaggcag cgatcatcag ctccaggttg cgctcggcca gcactacatc agagtatttc
     3661 cagttgagca cctctccctg ttcagtgatg cgaatctggc cctcgaaagc gcccacaggc
     3721 tgcgcataaa tcgagcgatg ggtggggccg ccgccacgtc ccacggtgcc tccgcgtccg
     3781 tggaagagac ggagcttcac gccgcattcg cgcgccactt cgtgcagggc gcggtgcgca
     3841 cggaagatct cccacgtgct ggtaagcatg cctccgtcct tattggaatc ggagtagccg
     3901 agcatgatct cctgtgtgtt gttccatgaa gccagcaggt tgcggtattc ggggatgttc
     3961 cagagcctgc ggcagacctc atgtgcattc cgcaggtcgc caatatactc gaagagcgga
     4021 acaggcataa ggcccgggtc gccgtttttc cccgcgcatt caacgccgcc aaggcgggcc
     4081 agccacagca ctccgaggat gtcttccacc gtgcttgcac cgctgatgac gtactgacga
     4141 atggcctccg gactgcatcc ccgtttgact tccgccacgg cccgaaatgt ctcgatcacc
     4201 cgggcgctct caggactgag gttctgcggg acggaggccg catcacccgc gcaccatgcc
     4261 gatgcttctt tcagcgtctt ctggtgcagg ttcacgtgct ggcggatgtc cagagtatgc
     4321 agatgcagtc cgaaggtgcg gaccagaaga atcagcgggt ctatgagggt ccgtgcaagg
     4381 cgcaggccct ggttttcggc aagactgcga cgcaggacgc gcagatcatc gagaaattcc
     4441 tgtgcgttgc agtaggcggg cagggcatgg agcgcggctt ccagagagtc tgtgtccgag
     4501 gcttctttcc cggctgtgcg caggaggcgc gcatgaacac agaccatgaa gcggcggtac
     4561 atctcgaatt caaagcgcgc gccgaaaacc tgttgcgttc ctgtgcggag ttccgagaga
     4621 taccggtgca gacgctggct cagctcttcg ctgataggca actgctgggc agacgtcgtc
     4681 agcagatcaa tgatgagctg cacctgcttt tcgtaataag ccagcaggcg cgtgcgtgca
     4741 gcgcggatgg catccagagt gacttcgggt gtgacaaatg gattgccatc gcggtcgcct
     4801 ccaatccagg agccgaagcc aagaagcagc ggcagctctg aaatatctat ctcgatgcca
     4861 tatacgcttg ccagcgcagc agagacttcc tcgtacagct tcggcagcgt cgcaaagatg
     4921 gagacatcgt agtagtcaag ccccatcttg atttcatcat tcaccgtagg ccgccggctg
     4981 cggacctcgt ccgtctgcca cagtgctgta atttccgcca gaagcgcttc ttccagacgc
     5041 gcaatttctt catcgggaat agggacgcgg tcgagctgct ccagaaattc tccaatacgg
     5101 cggcgtttga aaagcaccga gcggcgcgcc acctctgtgg gatgcgcagt aaatacaggc
     5161 acaataaaga tgcgacgcaa ccactcaagc gcctcgttgg ccgtaatacc gacgcgcttc
     5221 atctcctgca gcgtgccgcg caaagagccg cgctgtacct cgctgcctcc gcttaactgg
     5281 agcgagaggc ggcggcgttt gcggtggttg gtttccgcaa gattgatcag ctcgaaataa
     5341 aaggcaaagg cgcggctgag ctggtaagcc ctctccactg aaacttcagc gatgctgcgc
     5401 accgagcgct gcatcagctt ctcggcttcg gaagcattgc ctgcggcctg cgcttctcgc
     5461 cggcgaatgg cggtcgtgcg cagttcttcg actttttcgt aaagctcttc accggcctgt
     5521 tcgcgcaaca cctcgcccag caacatgccg agtgagcgca cgtcgcgtcg caatgggact
     5581 tctttcaggt cgccggaacg cgcctgaagt tcggccagcc gctgtggcca gctttctggt
     5641 ttccaaagaa ggggcatagt ttgattatgc cgcagcagga gtggcaactg aggtctaagc
     5701 agcgcatccg gcaaaaaagg aaggccgcat gggttgccgg aaagaaggcc acctctgtta
     5761 gagcttgatt tttttgggac tgaactccgg ctccggatat ttcatatcga gc
//
LOCUS       DASRBV010000256         8389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_223262, whole genome shotgun sequence.
ACCESSION   DASRBV010000256 DASRBV010000000
VERSION     DASRBV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223262"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFA02_09400"
     CDS             complement(<1..632)
                     /locus_tag="VFA02_09400"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZU10109.1"
                     /translation="MRAKTAVVLGAQWGDEGKGKIVDVLSGGFSAVARYAGGHNAGHT
                     VIIDGQKFILQLIPCGILRPGCKAVIGNGVVLDPMAFLKEVGKLRELGVDVDRHLFVS
                     NRAQVILEYHRMIELAAESAPGRQKIGTTSRGIGPAYEDKMARNGLRVVDLLNTNLLK
                     THIENACHEKNTIANALFGAKPLDPAKMYDEYARAAEQIAPFVTDTAALL"
     gene            911..1408
                     /locus_tag="VFA02_09405"
     CDS             911..1408
                     /locus_tag="VFA02_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU10110.1"
                     /translation="MSMERRQFLQIVPYVVGSGILLSHGEFALAQAVKAPENDASKGT
                     DMEKVAGIGGLFFRAHDPKALGNWYQQHLGVTLTPTTEGGSVWQQEAGPTAFTPFSEK
                     SSYFGDLNKTWMVNFRVHDMTKMVAQLRAAGIEVTDPESYPNIGHFARLHDPEGNPIE
                     LWQPA"
     gene            complement(1486..3522)
                     /gene="ligA"
                     /locus_tag="VFA02_09410"
     CDS             complement(1486..3522)
                     /gene="ligA"
                     /locus_tag="VFA02_09410"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZU10111.1"
                     /translation="MAQSTIAERIEELRETIRHHEHLYYVLDAPEISDAEYDKLVREL
                     EALEKQHPELITPDSPTQRVGGKPKEGFAKVQHSRPMLSLDNVTSEEELRDWDRRVRQ
                     LAGPKEEISYVCEYKLDGLSMALRYQEAKDGAAHLALGLTRGDGMTGEDVTTNVRTIR
                     SVPLSVSPAKLKKAKLPVNFEVRGEVVMPLAAFQKANEEREAQGLAPAANPRNYAAGT
                     IRTLEPNIVAQRRLDFYAYFALTPTGENIFAEQSEALDALTTAGFRVNPHREAIHSFE
                     KLMDFINRAEQHRATLGYEIDGVVIKVSSTELQQRLGYTGRAPRWAVAYKFTARAGIT
                     QVEDIKVQVGRTGKLTPVAVLTPVSIGGTTVSRATLHNADEIERLGLLIGDWVKVERG
                     GDVIPKVVEVLDDKEHPRGHKKFHFPKHCPDCGSEIVRTEGEADYRCVNTDCPARLRE
                     SLLHFASRGVMNIEGLGEAVVNQLLDRKLVRSIADVYKLDEAALLSLERIGEKTARSL
                     LREIEQSKKAPLNRVLYGLGIRFVGERTAQLLAEEFGSMDAIVSASQQELERVNEVGP
                     KVSQAIREFFDEEKNRQLVEHLREAGLTFTAEKRKKSSQLEGLTFVLTGTLPNLTRED
                     AKARIEAAGGRVSGSVSRKTSYVVAGEEAGSKLDKAHELKVPVIDEAKLLEMLG"
     gene            3799..3969
                     /locus_tag="VFA02_09415"
     CDS             3799..3969
                     /locus_tag="VFA02_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10112.1"
                     /translation="MWFLKMHDVLNHLSHLSDFLPAILITVGVAMLIGCAVGCVCTPC
                     EEKDDLFHKEVV"
     gene            complement(4022..4654)
                     /locus_tag="VFA02_09420"
     CDS             complement(4022..4654)
                     /locus_tag="VFA02_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HZU10113.1"
                     /translation="MTLQFHSAALLLAIDLIGTFVFAVEGAMAAAQNNLDILGLMVLA
                     FATALGGGIIRDVLIGATPPNSIRDWRYPATAFAGAILVFFQASLVSHFPALLLVTLD
                     AAGLALFAIAGAAKALDFKIHPFLAVLMGGVTGVGGGTIRDVLLAHVPNVLRADVYAV
                     AALAGAAIMVLALQLKARPAVATPLGIAVCFFLRMVAVWRHWNLPHVMAQ"
     gene            4772..5218
                     /locus_tag="VFA02_09425"
     CDS             4772..5218
                     /locus_tag="VFA02_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10114.1"
                     /translation="MKYCKPHLWLVVVLFGGMLATFAAPTLHAQRVSDRDVEAMMRNL
                     HEDAKTFRAGFDEELKKSTIRKTSQEKDARKLAEDFEKQTGEMLGEFRKTKKGDVAVE
                     NTMNSAEELGRIVERIQVSPRVASQWEKVRAELAQVSSAFGIHTSM"
     gene            5303..5890
                     /gene="frr"
                     /locus_tag="VFA02_09430"
     CDS             5303..5890
                     /gene="frr"
                     /locus_tag="VFA02_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZU10115.1"
                     /translation="MAGSAMAGIPALKEVHQQLKARMDKAVKDFQAHLASTRTGRANV
                     AMLDQVKVDYYGTPTPINQVGQLNTPDANTITIQPWDVSILGEIEKALRASDLGFNPQ
                     NDGKIIRVPVPPMTEERRREVVKHVSRVLEDHKTAVRNIRRDGNEMIRKAAKEKKISE
                     DEEKRSLEEIQKLTDEEIKRMDEMAKKKEAEIMQV"
     gene            complement(6254..7174)
                     /locus_tag="VFA02_09435"
     CDS             complement(6254..7174)
                     /locus_tag="VFA02_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZU10116.1"
                     /translation="MTKNLFFTGIILAASGLLSPPLALGAGLAYGMLLKHPYHLDSKR
                     LAKFLLQTSVVCLGFGMDLREVIQVGRSGFLYTAIGIAFALVLGALLGRLLRVGRTQS
                     FLISAGTAICGGSAIAALGPVTNANEEEMAVSLGTVFVLNSVALFVFPAIGAALHLSQ
                     TQFGLWSALAIHDTSSVVGAAAKYGAIALAVGTTVKLARALWIVPVALLMAVVKKSGA
                     RVHLPWFILYFCAAAVMSSYLPQLHVAYVAAYRLGKLGLAVTLFLIGTGITRQTLKET
                     GTRPMLQGVILWVVVASLSLLAIARGYVHL"
     gene            complement(7288..8181)
                     /locus_tag="VFA02_09440"
     CDS             complement(7288..8181)
                     /locus_tag="VFA02_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU10117.1"
                     /translation="MENFRLKVFRTVATQASFRKAAELLHLSQPAVSQHIQSLEEELG
                     LRLFDRSGVRISLTPAGKLLLRYAERAARVLDEAKTALLKLQGEVSGNLQLGASTTIA
                     QYILPRILGAFLKDNPRVKLSVLSGNTEEIVALLLKGAIMLGMIEGPPLSKEVHTEKF
                     LDDRMVLIAPRSHEWSGMSSVSLASLREVPLLLREQGSGSRRVVEQAIRKAGLSLNQL
                     HIAMELDSTEAILSGVEAGLGLGFVSEWAIAKALRLGAVSLVRVEGLEIRRHLMLVRK
                     SGPLPAGPTAFFWRFALAQNS"
     gene            complement(8245..>8389)
                     /locus_tag="VFA02_09445"
     CDS             complement(8245..>8389)
                     /locus_tag="VFA02_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZU10118.1"
                     /translation="VVAQRISIEDGAFFKGGIDIRKPGNEKAEVKPNSGATQTETAKA
                     VTV"
BASE COUNT         1898 a         2384 c         2354 g         1753 t
ORIGIN      
        1 ttcagcagcg ccgccgtgtc ggtcacaaaa ggagcaatct gctcggcggc ccttgcgtac
       61 tcgtcgtaca tctttgccgg atcgagcggc ttggcgccga agagcgcgtt ggcgatggtg
      121 ttcttttcgt ggcaggcgtt ctcaatgtgc gtcttcagca gattggtgtt cagcaggtcc
      181 acgacgcgca gtccattgcg ggccatcttg tcttcatacg caggtccgat gccgcggctg
      241 gtcgtgccga ttttctgccg tcccggggca ctctccgccg ccagctcaat catgcggtgg
      301 tattccagaa tcacctgcgc gcgattggag acaaagaggt gcctgtccac gtccacgccc
      361 aactcgcgca gcttgcctac ttctttgagg aaggccattg ggtccagcac cacgccgttg
      421 ccgatgacgg ccttgcagcc cgggcgcaga atgccgcagg gtatcagctg aagaatgaac
      481 ttctgcccgt caataatgac ggtgtgtccg gcattgtgtc cgccagcgta acgggccacg
      541 gcggaaaacc cgccggagag tacgtctaca atcttgcctt tgccttcatc gccccactgg
      601 gcgcccagaa cgactgctgt ctttgctcgc atcactgttt atgcctgctt tgcacacttc
      661 ccctcttgcg actggagcgt tcagatgact ggaacgaaaa gcaggcttga gggtctgtgt
      721 cagatcgtgg gcaaatgctc aacccaaatt ttgatgatat atgttttagt gcgcaacagg
      781 aaaggtgaga tgccggcata ctcgctctgt ctggtttcca gaaagaaacc ggcagactcc
      841 ggccactggt aaatggtgct ttgaggatga catttttgta ggtatgatgg gtcatctcgt
      901 gggagatgga atgagcatgg aaagacgcca gtttctgcaa atcgtgccat atgttgtcgg
      961 ctctggcatc cttctgtcac acggtgaatt cgccctcgca caggctgtga aggcgcctga
     1021 aaatgacgca tcgaaaggaa ctgatatgga aaaagtagcc ggaattggag gactgttctt
     1081 ccgggcgcac gatcccaaag cgctggggaa ctggtatcag cagcacctgg gcgtcacgct
     1141 tacacccaca accgagggcg ggagtgtctg gcagcaggaa gccgggccga ccgcgttcac
     1201 accattttcc gaaaaaagca gctatttcgg cgacctcaac aagacatgga tggtgaactt
     1261 tcgtgtccat gacatgacga agatggtcgc ccagttacgt gcagcaggaa ttgaggtcac
     1321 agacccggaa tcgtatccca atatcgggca ctttgcccgt ctgcacgacc ccgaggggaa
     1381 tccgattgaa ttgtggcagc ctgcctgaag agaatccggc cacgttctct cactttgcga
     1441 ctgcgcgtgc tctgccagat ttgcccggaa tagcaaaaca atgcgtcatc ccagcatttc
     1501 gagcagcttt gcctcatcaa tcaccggcac cttcagttca tgggccttgt ccagcttgga
     1561 gccggcctct tctccggcga ctacgtaact ggtcttcctg ctgacggagc ctgaaacacg
     1621 tcctcctgcg gcctcgatcc tagccttggc gtcttcacgc gtaagattgg gcagcgtccc
     1681 tgtcagaaca aaggtcaggc cctccagttg cgaggatttt ttgcgcttct cggcggtaaa
     1741 ggtcaggccg gcctcgcgca gatgctccac aagctgacgg ttcttctcct cgtcgaagaa
     1801 ctcgcggatg gcctgcgaga ccttcggccc cacttcgttc acgcgttcca gctcttgctg
     1861 tgacgcagac acaatggcat ccatcgagcc gaactcttct gccagcagtt gcgccgtgcg
     1921 ctcgcccaca aaccggatgc ccagcccata cagtacgcgg ttcagcggcg ccttcttcga
     1981 ttgctcgatc tcgcgtaata gggagcgcgc cgtcttttca ccgatgcgct caagcgagag
     2041 cagcgcggct tcatccagtt tgtaaacatc ggcgatactg cgaaccagct tgcggtcgag
     2101 cagttggttc accaccgcct cgcccagccc ttcaatgttc attacaccgc gcgaagcaaa
     2161 gtggagtaag ctctcgcgca ggcgcgcagg gcagtcggta tttacgcagc ggtagtcggc
     2221 ctcgccctcc gtgcggacaa tctcgctgcc gcaatccggg caatgcttcg ggaaatggaa
     2281 cttcttatgt ccgcgtggat gctccttgtc atccaaaacc tcgacaactt ttgggatgac
     2341 atcaccgcct cgctccacct ttacccagtc cccaatcagc aggccgaggc gctcgatctc
     2401 atcggcattg tgcagagtgg cgcggcttac cgttgttccg ccaatcgaaa ccggagtcag
     2461 cacggccacc ggagtcagct tgcccgtgcg tcccacctgt accttgatgt cttcgacctg
     2521 cgtaatgccc gcgcgcgctg taaatttata agccacggcc cagcgcggtg cgcggcctgt
     2581 gtaacccaga cgctgctgca attccgtgga gctgaccttg atgaccacgc cgtcaatctc
     2641 gtagcccagc gtggcgcggt gctgttcggc gcggttgatg aagtccatca gcttctcaaa
     2701 gctgtggatc gcctcgcggt gcggattcac cctgaacccg gccgtggtca gcgcgtccag
     2761 cgcctcgctc tgttccgcga agatgttctc gccagtgggt gtgagtgcaa aataggcgta
     2821 aaagtcgagt ctgcgctgcg ccacgatatt cggttccagt gtgcgaatcg tgccggcggc
     2881 gtaattccgc gggtttgctg ctggagccag gccctgcgct tcgcgttcct catttgcttt
     2941 ctggaacgca gcaagcggca tcaccacttc gccgcgtact tcaaagttca caggcaattt
     3001 tgcttttttc agctttgcgg gagagacaga cagcggcacc gagcggatgg tccgcacgtt
     3061 cgtagtcacg tcttcgccgg tcatgccgtc gccccgcgtc aacccgaggg cgagatgcgc
     3121 cgctccatcc tttgcttcct gatagcgcag cgccatagag aggccgtcca gcttgtactc
     3181 gcagacgtag ctgatctctt ccttcggccc ggcaagctgg cgcacgcggc ggtcccagtc
     3241 gcgcagctct tcttcgctgg ttacgttgtc gagcgaaagc atgggtcgcg agtgctgcac
     3301 cttggcaaag ccttcttttg gcttgccgcc gacgcgctgc gtaggcgaat cgggcgtaat
     3361 caactccgga tgctgctttt cgagtgcctc cagttcgcgc acgagcttgt catactccgc
     3421 gtcggagatc tcgggcgcat ccagaacgta gtaaagatgt tcgtgatggc ggatggtctc
     3481 gcgcagttct tcgatgcgtt cggcaatggt gctctgggcc atgagcatga ttataagaac
     3541 cgggcggcgt gcctgtggtg ccttcggtat tccagtgaaa aacctgctgg tttgaggtcc
     3601 ttcagggtga ccgtctctgg gcgggctgcc ccggcaactt atttcccggc ccaaccgtct
     3661 ttccctttat aggacttaga ggatttctgg ctgaaaattt atctcttccc aattgggaaa
     3721 gcgccgatgc atgatataga aaccagagaa aactatcagg ctggtttcgg gttctaaata
     3781 aatgggagct gtgtagttat gtggttcctg aaaatgcacg acgttctgaa tcatctgtcg
     3841 cacctgagcg actttctgcc tgccatcctg attacggtgg gggtagcgat gctcatcggg
     3901 tgtgctgtcg ggtgcgtctg cacgccgtgc gaagagaaag acgatctctt ccataaggaa
     3961 gttgtttaat ttctgtagat aacgccttca ataccgcccc acggcgatac ctgttttgtt
     4021 ttcattgcgc cataacgtgg ggcaggttcc agtgtctcca gaccgcaacc atgcgtagaa
     4081 aaaagcacac agcgatgccc aggggagtag caaccgcagg ccgtgccttt agctggaggg
     4141 ccaagaccat gatggccgct cctgccagcg ctgccacggc gtaaacatcg gcccggagca
     4201 cattgggaac atgcgccagc agcacatcgc ggatggtgcc cccaccgacc ccggttacgc
     4261 cccccatcag cacggccaga aaaggatgaa ttttgaaatc aagcgccttg gccgctcccg
     4321 caatggcaaa gagggcgagc ccggcagcat cgagcgtcac cagcaataat gctggaaaat
     4381 ggctcaccag gctggcttga aaaaagacca gaatggcccc ggcaaatgca gtggcaggat
     4441 agcgccagtc gcgaatggaa ttgggaggcg tggcccctat cagcacgtcg cggatgatgc
     4501 cgccgcccag cgccgtggca aaggcaagga ccatcagccc caggatgtcg aggttgttct
     4561 gcgccgcagc catggcacct tcgacggcaa aaacaaaggt gccgatcagg tctatggcca
     4621 gcagcagggc tgccgagtgg aattgaagcg tcacgtgttt atactttacg gtttcagttg
     4681 tataaaagaa taccgtcgtg gaaaacgatg cagcgtccac tgcatcgtac tgaaagtata
     4741 aaaacagaga aatatcagga gaatggcagg aatgaagtat tgcaagccgc atctatggct
     4801 ggtggtggtt ttatttgggg gaatgctggc cacatttgct gcgcctacct tgcatgcgca
     4861 gcgcgtaagt gaccgcgacg tggaagccat gatgcgcaac cttcacgaag atgccaaaac
     4921 gtttcgcgcg ggctttgatg aagagctgaa gaaaagcacc atccgcaaaa ccagccagga
     4981 aaaagacgcc agaaaactgg cagaggactt tgaaaagcag accggcgaaa tgctgggcga
     5041 gttcaggaag accaaaaagg gcgatgtggc cgtagagaac accatgaaca gcgccgaaga
     5101 gcttggacgc attgtagagc gcattcaggt aagcccgcgc gtggcctcac agtgggaaaa
     5161 agtgcgcgcc gagctggccc aggtgtccag cgccttcggc attcatactt cgatgtgaac
     5221 tgccccaccc gagatgacgc gcccccttcc agacaagtaa aatacagatt ttgcagcagc
     5281 ttgtaactaa aggaagacga caatggcagg ttcagctatg gccgggattc cggccctcaa
     5341 agaagtgcat cagcaactga aagcgcgcat ggacaaggcc gtaaaggact tccaggccca
     5401 tctggcatca acgcgcacag gacgcgccaa tgtggccatg ctcgatcagg taaaagtgga
     5461 ttattacggc acgccgacac ctatcaacca ggtggggcag ctcaacacgc ccgatgcaaa
     5521 cacgatcacc attcagccct gggatgtgag cattctgggc gagatcgaaa aggccctccg
     5581 tgcttccgat ctgggcttta atccacagaa tgatggcaag atcattcgtg tccctgtgcc
     5641 gccaatgacc gaagaacgcc ggcgcgaagt ggtgaagcac gtcagccgcg tgctcgaaga
     5701 ccacaagacc gcggtccgga atatccgtcg cgatggcaat gaaatgatca ggaaggcggc
     5761 aaaggagaag aaaatctctg aggacgagga gaagcgctcg cttgaagaga ttcagaagct
     5821 gaccgacgaa gaaatcaaac gcatggatga gatggcaaag aagaaagaag ccgaaatcat
     5881 gcaggtataa gctggcgtag tccagggtcc atatcagcct ttctctttgg gcgtgtgagg
     5941 aaaaaggact gcgcacaagc ccggacacaa gccgcgtgtg agcgacgggt gagcaggacg
     6001 agcatttctt tggctgaagc ggctgcacag ggactgtacc cgtgtgaaac tttcagcgca
     6061 tgggcgcaag gcaggacgag ttccacagca aagaaaagca taaagaaaag gcaacgggga
     6121 cctctgacga tctgcaggca gacctcggtt cctgatttgc ctggcatggc ttctcgggcc
     6181 cgccgagtgt gccttgtcca cgcttcgggc agtctcagcc atgccgtaac tccctgcctg
     6241 attcaacaac gcgctaaaga tggacatagc cgcgtgcaat cgcaagcagt gacagcgacg
     6301 ccactaccac ccatagaatg acgccctgta gcatcggcct ggtcccggtt tctttcaggg
     6361 tctggcgtgt gatgcctgtt ccaatgagaa agagcgtaac ggcaagccct aattttccca
     6421 gcctgtaggc ggcaacataa gcgacatgga gctgaggcag gtagctgctc atcacagcag
     6481 cggcgcagaa atacagaatg aaccacggca ggtggacccg cgcaccactc tttttcacca
     6541 cggccatgag caaggcaacc ggaacgatcc acaacgcgcg cgccaatttg accgtcgtgc
     6601 cgactgccag tgctatcgcg ccatacttag cggcagcacc tacgacggag ctggtgtcat
     6661 ggattgccag cgctgaccac aaaccaaact gcgtctgcga aagatgcagt gctgccccta
     6721 tggcgggaaa aacaaaaagc gccacagagt tcaaaacaaa gactgttccc aacgagaccg
     6781 ccatttcttc ttcgttagca tttgttaccg gaccgagtgc ggcaatggcg ctgccgccac
     6841 agatggcagt tcctgcggag attaagaaag actgtgttct gcctacacgc agcaaacgtc
     6901 ccagcagcgc ccccagtaca agcgcaaatg caataccaat ggcggtgtaa aggaaacccg
     6961 agcgtccgac ctgtatgact tcccgcaggt ccatgccgaa acccaggcat accaccgagg
     7021 tctgaagcag gaatttcgcc agccgcttgc tgtccagatg gtagggatgc ttcaacagca
     7081 ttccataggc cagaccagca cccaatgcca gaggtggcga aagcagcccg cttgcagcca
     7141 aaataattcc ggtgaaaaag aggttcttcg tcatacactc ctgagcttgc caactggagc
     7201 gcggtcacgt ccaagagtga tttgtgatga gtgattcgtt ttgcttatct gctctgtcca
     7261 gcgaagctcg tctcgcaaaa aatggattca ggagttctgt gcgagagcaa agcgccagaa
     7321 gaatgcagtc ggccctgcgg gcaaagggcc actcttccgc accagcatta gatggcggcg
     7381 gatttccagc ccttccactc gcaccagaga aactgcgccc aaccgtaatg ccttggcgat
     7441 ggcccactcc gaaacaaagc cgaggccgag ccctgcttca acaccggaca ggatggcctc
     7501 ggtggaatcc agttccattg cgatgtgcaa ttggttcaaa gataaaccgg ctttgcgaat
     7561 cgcctgctca acgacccgtc gtgagccaga accttgttcc ctcaggagca gtggcacttc
     7621 cctcagcgat gccagtgaaa cactcgacat cccactccac tcatggctgc gtggggcaat
     7681 gagcaccatg cggtcatcga ggaacttctc agtatggact tccttcgaca gcggtgggcc
     7741 ttcaatcata ccgagcatga tggcgccctt cagcaacagg gcgacaatct cttccgtatt
     7801 gccgctcaag acggagagtt tgactctggg gttgtccttt agaaatgcgc ccaggatacg
     7861 tggcaggatg tattgtgcaa tggtagtgga agcgcctagc tgcaggttgc cgctcacctc
     7921 gccctgaagt tttagtagcg ctgttttggc ctcatcaagt acgcgggcag cacgttccgc
     7981 atacctgagc agtagtttgc ctgcaggagt taacgaaata cgcacaccac tgcgatcaaa
     8041 gaggcgtaag cccaattctt cctcaagcga ctggatgtgc tgactcaccg caggctggct
     8101 gagatgcaat aattccgccg ctttgcggaa gctggcctgc gtggccaccg tgcgaaagac
     8161 tttgagccgg aaattttcca tcaggtctta aacaaaagac cccggaggat tccggggtct
     8221 tttatgtttc aggataaagc cagcttacac cgtaacggcc ttggctgttt ccgtttgcgt
     8281 agcgccagag ttcggcttga cttcggcctt ctcatttccg ggcttgcgga tatcaatgcc
     8341 gcccttaaag aaggcgccat cttcaatcga aatgcgctgt gctacgaca
//
LOCUS       DASRBV010000257         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_15738,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000257 DASRBV010000000
VERSION     DASRBV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15738"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFA02_09450"
     CDS             complement(<1..435)
                     /locus_tag="VFA02_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10119.1"
                     /translation="MPDHQIDYHNWKPKHNPWLVALTVTIATFMEVLDTSIANVALPH
                     MAGSLGASEEEATWVLTSYLVSSAVVLPISGWLATRFGRKRFYMACVAIFTICSFFCG
                     IAPSLPLLIFARVLQGAGGGGLAPSEQAILADTFPVSKRGQAF"
     gene            513..800
                     /locus_tag="VFA02_09455"
     CDS             513..800
                     /locus_tag="VFA02_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZU10120.1"
                     /translation="MYIHCFAFRWKPGVTEDQIRRAESEIKKLQGQIPGLLETYVGRN
                     ISPRGQGYTFGGVMKFADKASLDAYGPHPVHQQLVRWLMPLIDPIEIDFEI"
     gene            955..3480
                     /locus_tag="VFA02_09460"
     CDS             955..3480
                     /locus_tag="VFA02_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10121.1"
                     /translation="MRFAFLQDLRYALRQFRKSPGFTLTAILTMALGIGACTAIFSLF
                     DQALLRSLPVPNPQQLVQLRFSGATVGHRSTQGGDSPGAFAYFSYPMYRDLRDKSALH
                     DLFATAVGNAGISHQSGPDASAEAVPVEMVSGNYFSVLGVHPAAGRLFTAADETVSGG
                     DPYAVLSFDYWKARFNEDPQIIGHSISVNGTPFTVVGVTAPGFQSVVWGDMPRLFVPI
                     TMKRIITPEWDDLNDRQSYWLKIMGRLPAGTTMRQAGAMLNPLWLSLRKMEFGNLRDQ
                     SANAYQGFVQKAHLDEISAAKGFSPMRDDVEMPLKILMGMVLLVVAMACVNMASLLLV
                     RSAGRAREFSMRYALGASKSAVVRQLLTEGLLLGFGGAAVGILLAPQMVHMLIAWISG
                     TTSDTPFSPELEPRILAFAITTTLVVSLLFSLAPALQLLKPNLVESLRQQSSTGTGSA
                     LRFRRTCVALQIGFSLMLLVGAGLFMRTIQNLRSVNAGFATDHLLQFDFAPETAGYQG
                     EAVTAVEQRVLDALAALPGVRGVAATNDPELANDNVAGSMDIAGYTPAPNEDVDAEIP
                     WVSQDYFSAMGIPLVAGRGFTKADAPDAQKVAVVNEMFARKYFGTPQNALGHHVSRSH
                     RPATDTVIVGVVKDMKHQSVRDKVLPTVYRPFVQGEKPTSLIVYVRTWQTPESTSSAV
                     RAAVHNIDAKLIVGDLRTMQNQIDQSISSERLMALLATLFGLLAAALAGIGLYGVLAY
                     STAQRTREIGIRMALGAQRGMIAKLVLREVLVLAAGTVIVTLPLAWLLGRTLRNQLFG
                     VSPADPVVYFGGILMIGMVALFAAFLPARRAASVEPMQALRAE"
     gene            3498..6095
                     /locus_tag="VFA02_09465"
     CDS             3498..6095
                     /locus_tag="VFA02_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10122.1"
                     /translation="MPVIWNDVRYALRQLRKSPGFTATAVLTLALGIGVTTAIFTLVQ
                     QVMLKSLPVTKPEELWRIGDRVRCCNWGGYTQGDDVAPNDFTLFSYEAYQNFRAHTPE
                     FTDLAALEAGSIPLGVRRAGTSQQAQNQNGEFVSGNFFRTFGVGAWIGRVFTDADDVE
                     GATPVAVMSHHVWQQKYASDSSVVGATFLINGHPFTVIGVAPPGFYGAKLSDGNMPDF
                     WLPLATEPLLDSLTRGQSGVRLKSPDTNWLDLIGRVRPGTNTKMLEAKLRVELHDWLA
                     SHVGDMGPQEKETWQKQTLRLTPGGAGVADMRQNYGDGLKLLLAASGCVLLIACANIA
                     NLLLARGLKNRHQNSVRAALGASPKRLVYKALVESITLGIIGGVFGIAVAYGGTRLML
                     HLAFAEVAKTSFVPIQASPSIPVLLFSLVVSVFTGILFGIAPAWLVARANPVEALRGV
                     GRSVSGRATWPQKSLVIVQAALSLVLLSAAVLLGQSLRNLEHQNFGFDTRGRYIARIN
                     PELAGYKSDQLDMLFRRIQERLGAIPGVRGVSAALYAPMSGDSWNNSIRVEGKPEPGP
                     KEDASAGIVRVTPGFFETLGDRMLMGRPITDEDTATTRKVAVINEAFAKRFFKGEDPI
                     GKHFGPSKMKYAATYEVIGVASDMRYMTWDMKDSPVPMYFVPETQTAQWDDASFIAAE
                     NWSHYLNNIILWAPGNPPGLEAQVRKALGEIDPDLVLYSVDSYASVLDSDFSQQNMIA
                     TLTSLFGLLALVLAAIGLYGVTAYNVEQRTTEIGIRMALGADRSSVLKMVMRSAFLQV
                     AIGLVIGIPAAIGAGYAISGQLFSVKPWDPVVLVLATLMLAMAAFVAAMVPSRRAAGI
                     EPMQALRME"
     gene            6292..>6799
                     /locus_tag="VFA02_09470"
     CDS             6292..>6799
                     /locus_tag="VFA02_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HZU10123.1"
                     /translation="MKKKIATALFVFSVSVFGFAQSSRPALLPQPREFQGREDISLAQ
                     GVFISVPGRDKDDAFAARDLADGLKELGVSVAARAGAAHVYLLRDTSVAGKRVLSEAK
                     QSFDQPMLDEGYVLVASKRSVYVIGHTAAGVFYGAQTVKQLVSGTGGDAVLHGCVIRD
                     WPAMKYRGV"
BASE COUNT         1388 a         1845 c         2074 g         1492 t
ORIGIN      
        1 aaaagcctgt ccccgcttgc tgacgggaaa cgtatcggcg aggatggcct gttcgctggg
       61 ggcgagtccg cctccgcctg caccctgcaa aacgcgcgcg aagatcagca gagggagaga
      121 tggcgcgatt ccgcaaaaaa acgagcagat agtaaagatg gccacgcacg ccatgtagaa
      181 gcgcttgcgt ccaaagcgcg tggccagcca gccggagatg ggcaacacca ccgcgcttga
      241 aaccagatag ctggtcagca cccatgtagc ttcttcctcg ctggcgccaa gcgagccagc
      301 catatgcggc aatgcgacgt tagcaataga ggtgtcgagg acctccataa aagtggcaat
      361 cgtgaccgta agcgcaacca gccaggggtt atgcttcggc ttccagttat ggtagtcaat
      421 ctggtggtcg ggcacggcta ccgtcgcttc gcaggatagg atgaaattgg gttaaccata
      481 gagtaacaag acagatgatt ccaagagggc ctatgtatat tcattgcttt gcctttcgct
      541 ggaaaccggg agtcacggaa gaccagatac gtcgcgctga aagtgaaata aagaagctgc
      601 aggggcagat tccagggctg ctggagacat atgtgggcag gaacatctct ccccgcggcc
      661 aggggtacac ctttggcggc gtgatgaagt ttgccgataa agcaagcctg gatgcttatg
      721 gcccacatcc cgtacatcag caactggtgc gttggctgat gcctttgata gatccgattg
      781 aaatagactt tgaaatttag tgtccatttc cggacgccct gtgctcgaat ctggacagtg
      841 atttgtggat gaggatgcgg cgcaagggga ttcaatcttt tagattgaac aacttgaccg
      901 ttttgccgtt ctggcatccg acgtgcattc attcgcattg aggagtctgg ttcgatgcga
      961 ttcgcgtttc tgcaggactt gcgctatgca ctgcggcaat tcaggaagtc tccgggattt
     1021 acgctgaccg ccattctgac catggcgctt gggattggcg cctgcacggc catcttttcg
     1081 ctctttgacc aggccctgct gcgctcattg cctgtgccca atccgcagca actggtgcaa
     1141 ctgcgatttt ccggggccac ggtggggcac cgcagtacac agggcggaga cagcccaggt
     1201 gcctttgcgt acttttccta tccgatgtac cgcgatctgc gagacaagag cgcccttcat
     1261 gatctttttg cgactgctgt aggaaacgcc ggaatcagcc atcaatctgg cccggacgca
     1321 agcgcggaag cggtcccggt cgagatggtc agcggcaatt atttcagtgt gctgggcgtg
     1381 catcctgccg ccggacggct gtttactgcg gcggatgaga cggtctccgg cggcgatcct
     1441 tatgccgtgc tcagtttcga ttactggaag gcgcgcttta acgaagatcc gcaaatcatt
     1501 ggccacagta tctctgtcaa cggcactccc tttactgtag tgggcgtgac ggcccctggt
     1561 tttcaaagcg ttgtctgggg agacatgcca cgcctctttg tccccatcac gatgaagcgc
     1621 atcatcactc ccgagtggga cgacctgaat gacaggcaat cgtactggct taagatcatg
     1681 ggccggttgc ctgcgggaac gacgatgagg caggcaggcg ccatgttgaa tccgctatgg
     1741 ctctcactca ggaagatgga attcggcaat ctgcgcgacc agtctgcgaa cgcataccag
     1801 ggttttgtcc aaaaggcaca cctggatgaa atcagcgcgg caaagggctt ttcgccgatg
     1861 cgggacgatg tagagatgcc gctgaaaatt ctgatgggca tggtgctgct tgtcgtcgcg
     1921 atggcctgtg tgaacatggc cagtcttctg ctggtgcgct cggcaggtcg cgcgcgcgag
     1981 ttttccatgc gctatgctct cggcgcgagt aagtctgccg tggtgcggca gcttctgacc
     2041 gaggggctgc tgctggggtt tgggggcgcc gcggtgggga ttctgctggc cccgcagatg
     2101 gtacatatgt tgattgcctg gatctctggc acaacgtcag acacgccatt ttcaccggaa
     2161 ctcgagcccc ggattctggc ctttgccatc accaccaccc tggtcgtgag tttgctgttc
     2221 agtcttgcgc cagcattgca actgctgaag cccaatctgg tggagtcact caggcagcag
     2281 agcagcacag gaacaggcag tgcgctccgg ttccgccgga catgtgtggc attgcagatt
     2341 ggtttcagcc tcatgctgct ggtgggtgca ggattgttca tgcgcaccat tcagaacctg
     2401 cgctcagtga atgctgggtt tgccaccgac cacctgctgc aatttgactt tgcgcccgag
     2461 acggccggct atcagggcga ggcagtcacg gcagtggagc agcgtgttct cgatgctctc
     2521 gcagcgcttc cgggcgtgcg cggagtggca gccaccaacg atccggagct ggccaacgac
     2581 aacgtggccg gctcgatgga cattgccggt tacacacccg ctccgaacga agatgtggat
     2641 gctgagatcc cgtgggtgag ccaggattat ttctcagcca tgggcattcc cctggttgca
     2701 ggaagaggat ttacaaaagc cgacgcgccc gacgcgcaga aggtcgcggt agtgaatgaa
     2761 atgttcgctc gcaagtattt tggcactccg cagaatgctc ttgggcacca tgtaagccgc
     2821 agtcatcgtc cggcaacaga cacggtgatt gtaggggtgg tgaaggacat gaagcatcag
     2881 agcgtgcgcg acaaagtgct cccgaccgtg taccggccct tcgtgcaggg agaaaagccc
     2941 acatcgctca tagtttatgt gcggacgtgg cagacgccgg aatccacgtc cagcgccgtc
     3001 cgcgcggcag tgcacaacat agatgcaaag ctgattgtag gtgacctgcg caccatgcag
     3061 aaccagatag accaaagcat cagttcagag cgcttgatgg ccttgctggc gacactcttc
     3121 ggattgttgg ccgcagccct ggcaggtatt ggcctgtatg gtgtgctcgc ttactcgacg
     3181 gcccagcgca cgcgggaaat tggcatccgc atggcgctcg gcgctcagcg gggaatgatt
     3241 gcaaagctgg tcctgcgcga ggtgctggtg ctggctgcgg gcacggtcat tgtgacgctg
     3301 ccgcttgcct ggctgctggg ccggacgctg cgtaatcagc tctttggcgt atcgcctgcc
     3361 gaccccgtgg tctatttcgg cggcattctg atgatcggaa tggtcgcatt gtttgcggcg
     3421 tttctgcctg cgcgcagagc agcgtcggtg gaaccgatgc aggcactgcg ggcagagtaa
     3481 ctcctggaga atttgttatg cctgtgatct ggaatgacgt tcgttatgcg ctgcggcaac
     3541 tgcgcaagtc tcctggattt actgcgactg cggtgctcac gctcgccctg ggaatcgggg
     3601 tcacaacagc gattttcacg ctggtgcaac aggtaatgct gaaatctctt ccggtcacga
     3661 agcctgaaga gttatggcgg attggcgaca gggtccgctg ctgcaactgg ggcggatata
     3721 cacagggcga tgacgttgct ccgaacgact tcacgctctt ttcctatgag gcatatcaga
     3781 actttcgcgc gcacacgccg gaatttacag acctggccgc gcttgaggct ggaagtatac
     3841 cgcttggcgt gcgccgcgcc ggcacatcgc agcaggcgca gaaccagaac ggcgaatttg
     3901 tctccgggaa cttcttccgc acctttggag tgggagcatg gattggccgc gtgtttacgg
     3961 atgccgacga tgtggaaggc gcaacaccgg tcgctgtcat gagccatcac gtctggcagc
     4021 agaaatatgc ttctgattct tccgttgtag gcgcgacgtt tctgatcaat ggtcatccat
     4081 tcacggtcat cggagtggcg cccccaggat tttacggggc caaactgtct gacgggaaca
     4141 tgcctgattt ctggctccct ttggcaacgg agcccctgct ggacagcctg acgcgcggac
     4201 agagcggcgt caggctgaag tctccggaca ccaattggct ggacctgatt ggacgggtcc
     4261 gcccgggcac gaacacaaag atgctggaag caaaactccg cgtagagctg catgactggc
     4321 tggcaagcca cgtgggcgat atggggccac aggaaaaaga gacctggcag aagcagacgc
     4381 tgcgcctcac gcctggcgga gcgggtgttg ctgatatgcg ccagaattat ggcgacgggt
     4441 tgaagctgtt actggccgcc tcaggatgcg tcctgctgat tgcatgcgct aacatcgcaa
     4501 acctgcttct ggcccgtggt ctgaaaaacc ggcaccagaa ttcggtacgg gctgcgttgg
     4561 gcgcatcacc aaagcggctg gtgtacaagg cgctggtgga aagtatcacg ctgggcatca
     4621 tcggcggtgt attcggaatc gcggtcgcct acggaggaac acggctgatg ctgcatctgg
     4681 cttttgcgga agtggcaaag acgagcttcg ttccgattca ggccagcccc tcgattccgg
     4741 tgttgttgtt ttctctggtt gtttctgtat tcacggggat tctgttcggc atcgccccgg
     4801 catggcttgt tgcccgcgcc aatccggtgg aggcattgcg gggcgtgggc cgttccgtca
     4861 gcggcagggc cacatggccg cagaagtctc tggtcatcgt tcaggcagcg ttgtctctcg
     4921 tgttgttatc ggccgcagtg ctgttaggcc agagcctgcg caatctggag caccagaact
     4981 tcggttttga cacaaggggc cgctacattg cccggatcaa tcccgagctg gcgggatata
     5041 agtctgatca actcgacatg ctcttccgcc ggatacagga gcgactgggc gcgattcccg
     5101 gtgtccgcgg cgtaagcgct gctttgtatg cacctatgag cggggacagc tggaacaaca
     5161 gcattcgcgt ggagggaaaa cccgagcctg ggcctaaaga agatgcatcc gcaggtatcg
     5221 tccgggtcac gcctgggttc tttgagacgc ttggagaccg gatgctgatg ggccgtccaa
     5281 tcacagatga agatacggcc acaacgcgca aggtggccgt catcaatgag gccttcgcga
     5341 aaaggttttt caaaggcgag gaccctattg gcaaacattt cggcccaagc aaaatgaaat
     5401 atgcggcaac ctatgaggtc atcggcgttg catcggacat gcgctacatg acctgggaca
     5461 tgaaagattc gccagtgccg atgtacttcg tcccggagac gcagacggcg caatgggacg
     5521 acgcaagctt cattgcagca gaaaattggt cacattatct gaacaacatc attctatggg
     5581 cacccggaaa tccgcccgga ctcgaagcgc aggtgcgcaa agcgctgggc gagattgatc
     5641 ctgacctcgt gctctacagc gtagacagct atgcgtctgt tctggattca gacttttcgc
     5701 agcagaacat gatcgctacg ctgacatcgc tcttcggtct gctggccctg gtgcttgcgg
     5761 ccatcggact ctacggcgtc accgcttata acgtggagca gcgcacaact gaaatcggca
     5821 ttcgcatggc cctgggtgcc gaccgctctt ccgtgctgaa gatggtgatg cgcagcgcgt
     5881 tcctgcaggt tgcgatagga ctggtgattg gcattcctgc cgccatcggc gctggatacg
     5941 ccatcagcgg ccagctcttt tcagtcaaac catgggaccc ggtggtcctg gtactggcga
     6001 cattgatgct ggcgatggct gcattcgtcg cagccatggt cccatcgcga cgggcagccg
     6061 gcatcgagcc aatgcaggca ttgcggatgg aataggtaag cccaggtctc ctgggtttat
     6121 tgactggccc cgaaagatgc acattgactt cactctgctg tcaacgtata gtcttctatc
     6181 aaagtaaaca agtcagctta agagcacaca gaatggattc tggtgctctt cttctatcgg
     6241 cataaccctg agcgcggggg gctgaatcac gacagctcca gaggaccttg cgtgaaaaaa
     6301 aagatcgcca ctgctctttt tgtgttttct gtctctgtct ttggatttgc ccaatcgtcc
     6361 cgcccggcgt tgctccccca gccgcgcgaa tttcagggca gggaagacat ttctcttgcc
     6421 cagggagtct tcatttccgt tcccggcagg gacaaagacg acgcgttcgc ggcgcgagat
     6481 cttgcagacg gcctcaaaga acttggggtt tccgttgcag cacgcgcggg cgcggcgcat
     6541 gtttatcttt tgcgcgatac gtctgttgca gggaaacgcg ttctgtctga ggcgaagcag
     6601 tctttcgacc agccaatgct ggatgaaggc tacgttctgg tagcgtccaa gcgaagcgtc
     6661 tacgtgattg gacatacggc cgcaggcgtc ttctacggcg cgcagaccgt gaaacagcta
     6721 gtttccggaa caggcggtga cgcggtgctg catggatgcg tcattcgcga ttggccggca
     6781 atgaaatatc gcggcgtgc
//
LOCUS       DASRBV010000258         9820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_578833, whole genome shotgun sequence.
ACCESSION   DASRBV010000258 DASRBV010000000
VERSION     DASRBV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578833"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..740
                     /locus_tag="VFA02_09475"
     CDS             36..740
                     /locus_tag="VFA02_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU10124.1"
                     /translation="MIELKNVERSYKTGAGQTWVLRRINLTIHEGEFVTIMGPSGAGK
                     SSLLNVLALLDDQWLGEYWFKDEPVHKMNRKQRAELAKRNIGMVFQSYHLLDDLTVQE
                     NIDLPLSYKDIPRAERQALVADTLDHFQIVGKKDLYPSQLSGGQQQLVGIARAVIHKP
                     ALLLADEPTGNLHSSQAKEIMELFKQLNEEGTTIVQVTHSEVNAAYGSRIIEVRDGWM
                     VKDTANPQAGAEVSAS"
     gene            737..2734
                     /locus_tag="VFA02_09480"
     CDS             737..2734
                     /locus_tag="VFA02_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU10125.1"
                     /translation="MKKRPGSQQQLAAVALSFLMVASGLAQTAQQPAPSSVPDAPSAT
                     QQHAPLSIAQQAQQAQQVTPVGQQAPFHVEVPHSHNPFAPYMPSTVPPINLTNSPRLQ
                     SLVRDGKLYLSLHDAIALALENNLDLAYFRYNLPIAQADLLRTKAGGSANGVNTAIAQ
                     GTQGGFSASGVGNGGGGSSSGATAAGAGGLVTSTLGNGASIPSFDPHLNVQGFVDHTT
                     LVQVNTIQYGVPILKQNTIQLQSSYSQAFSLGTNFTITDYGLRQTTNSLYNILSPQLT
                     TNFNLVIAQPLLQGFGLATNQRFMHIAKKNIQLTDLGFRAQVIATITQVENIYWDLVS
                     AYKDAQVKERSLAYANETLSDDQKQLQLQAIPAMQVMKDQAAVATAEGDLTVARATLK
                     LNELLIKNALTKTIDDPALADMPVIPLDLTGTSDANAEKPIEQLITEAEKNRPDVAED
                     QIAMQIAQNNLKTIKNELLPRLSLYGQFVGAGFGGQLNRYCALGSECNTTLPKDFAGA
                     FENTFNYSSPEYQVGFSLDITLRNRIAKADQFRAVLDYRQKELTFEQQKKSILLDVRN
                     SQYALQQAQARVVAAQKARDLAQKTFDISKQEQKLGAMSAYDVLTSEQALAVAESALA
                     VAQSAFEKAKVDIDRATGSTLDRMGVSIDDAKSGVVAHMQP"
     gene            2830..4233
                     /locus_tag="VFA02_09485"
     CDS             2830..4233
                     /locus_tag="VFA02_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU10126.1"
                     /translation="MSTTYQAGRLETEAAAAQPSRVLIADDQPSILDALELLLTPEGY
                     QIEKARSPMAVLGALQSGAFDALLVDLNYTRDTTSGKEGLDLLTHIKSLDSQVPVIVM
                     TAWANVEVAVEAMRRGARDFIQKPWDNARLLTILRTQIELHHAVRRAQHLEAENQMLR
                     GQGLPELIATAPAMQPVLSTIRRIGPSDANVLITGEHGTGKEVVAQMLHALSPRAARS
                     LVAVNTGALPEGTFESELFGHVKGAFTDARTERIGRFELADGGTLFLDEIANVPLRQQ
                     AKLLRVLETGEMERVGSSKTRRVNVRVLSATNADLRAEAEQGRFREDLLFRLNTVEIH
                     LPPLRERREDIPLLAGHFLNRYAGRYRKPMEGIDPAAVQVMMQHPWPGNVRELDHTIE
                     RAVLMARGTKIEASDLGLNQVRVTAQTLEDMSLEAVESILIRKALARASGNVSQAAEA
                     LGLSRGALYRRMEKYGI"
     gene            4223..5650
                     /locus_tag="VFA02_09490"
     CDS             4223..5650
                     /locus_tag="VFA02_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU10127.1"
                     /translation="MASDRGHATSKIPAEGFRFETRVKILIASLCLPVFVLIAVLLWI
                     EHSSLPVIVGTLTALALVVLLGASIFIEQVVRPLQTLTNVVAALREEDFSFRARNATP
                     HDALGELAIEINQLADTLQSQRLGALEAVALLRRVILEMDAPVLAFDEQCTLRVVNPA
                     AERVLRLNAARDLGRKAGDLGLQRLIDEADEGIVAIESQGQQARWMVRKSSFRQRGVP
                     HTLLVLSDVSAALREEERAAWRRLIRVLGHEISNSLAPIKSIAGSLRQRIGQVKDGNT
                     ADFERGLNVIESRAESLNRFVQAYRHLAQLPAPVLRSVSLPLLFDRVAGLETRLPIEI
                     GDVPQVNLMADADQMEQLLINLVRNAVEAAISEHEEGGMAGSPAVRISGRVAGGFASI
                     LIEDNGPGLANPGNLFVPFYTTKRTGTGVGLVLARQIAEAHGGSLELRNRAGTKGCQA
                     EVRIPLVGERDRVDEARASAAEHDG"
     gene            5877..7136
                     /locus_tag="VFA02_09495"
     CDS             5877..7136
                     /locus_tag="VFA02_09495"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZU10128.1"
                     /translation="MSEAVSKVAILGFGTVGSSVARILCDAKPQGVELTHVFNRGVAR
                     KRVEWVPAGVTWTENIEDVFSSSADVVLELVGGLEPTGEWVRRALEAGKSVVTANKKL
                     IAEHGIALDKLARRKGVHLYYGAAVAGGVPVIPGIQQGLAGDSITRIEGILNGTCNFI
                     LSKMEEGAEFAPVLKEAQKLGYAEADPTEDVGGFDARAKLVILSRIALRADVAVGDVC
                     CKPITEIAAIDFAYAREMGCTIRQISRAELKDGQVWAAVGPMLVPLASPLAWSRGTEN
                     MVLVGGAYGGDVVFSGHGAGGHPTAVAVVSDLLAVVHGSRTIDLPSRKTPVSADLLVP
                     HYIRFIVKDRPGIIAQIAGALAEQEINIHAILQKPGYKKTNLPFVVIAEPCSGSALKR
                     ALEKIGHLDCLLEPTLDMPILDAESLS"
     gene            7306..8385
                     /locus_tag="VFA02_09500"
     CDS             7306..8385
                     /locus_tag="VFA02_09500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10129.1"
                     /translation="MSTQAMSRSVDPDKMQALLNQAVADMGAAMHSVLLVIGDRLGLY
                     RVMASGEPMTPAELARRTGTFERYVREWLNANAAAKYVEYDAASGMYFMTPEQAQLLS
                     QGGMAMDLPGLYHFIASCMKDEEKITEVFRTGKGFGWHEHEKGLFEGTERFFRPNYLA
                     NLTTNWIPALDGVEAKLRQGARVADIGCGHGASTILMAQTYPKSEFFGFDYHEASIER
                     ARKTAAEAGLSDRVKFEVAPAQAYPGENYDFAACFDCLHDMGDPAGAARHVLQSLAKD
                     GTWMIVEPRAGDDTVANLNPVGKIFYSASTICCVPASLSQEVGAGLGAQAGEKKIREV
                     VMQGGFTHFRRAAETPFNMVFEARR"
     gene            8556..9347
                     /locus_tag="VFA02_09505"
     CDS             8556..9347
                     /locus_tag="VFA02_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10130.1"
                     /translation="MRILRSVTLLAALALPASFAHGQIAVGIGIGPVIDYPAPVYVAA
                     PPVCPWGYYPYWPYECAPYGYYGPDWFVSGVFIGAGPWFTGWFGAPWGWGWGHPWGGW
                     GWGHPYWGYGRGYGWGYTSGYSHGYVNGFNNGYRNGGAWGVPQSRGFNNGFRGGGAYG
                     HTPLGGTHVLNSAGYRAPYNGGFRGSNGFHTFTGSTGFHGTGSTGFHGYSGSSGFHGY
                     SGGGMSGFHGSSGMHGFGGGGFHGGGFGGGGFHGGGGFHGGGGHR"
     gene            complement(9556..>9820)
                     /locus_tag="VFA02_09510"
     CDS             complement(9556..>9820)
                     /locus_tag="VFA02_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease La domain-containing
                     protein"
                     /protein_id="HZU10131.1"
                     /translation="ECLALHFEILELAGIEQPSVQLDLGAPIAFHLAAALPADLGFKQ
                     QLLVLRSDAERTRMLCDFYRVMLPKLRAGAHANRVASGNGRVM"
BASE COUNT         2050 a         2416 c         3260 g         2094 t
ORIGIN      
        1 tgcaatttgc atcgcgcaac tcaggatgat gtttcatgat tgaactgaaa aatgtagaac
       61 gcagttacaa gaccggggct gggcagacat gggtgctgcg ccggatcaac ctcaccattc
      121 acgaaggcga gtttgtgacc attatggggc catcgggggc ggggaagtca tcactgctga
      181 atgtgctggc gttgctcgat gatcagtggc tgggcgagta ctggtttaag gacgagcctg
      241 tacacaagat gaaccgcaag cagcgcgccg agctggcgaa acggaacatc gggatggtgt
      301 ttcagagcta tcacctgctg gatgatctga cggtgcagga aaatattgat ctgccgcttt
      361 cctataaaga cattcctcgc gcagagcgcc aggcgctggt ggccgacacg cttgatcatt
      421 ttcagattgt gggcaagaaa gacctgtatc cgtcacagct ttcaggcggg cagcagcagc
      481 ttgtggggat tgcccgcgcg gtgatccata agcctgctct gctgctggcc gatgagccga
      541 cggggaacct gcattcgagt caggcgaaag agattatgga gctgttcaag cagttgaatg
      601 aggaggggac gacgatcgta caggtgacgc actcggaagt aaacgccgcg tatggcagcc
      661 gcattattga agtacgcgat ggctggatgg tgaaggacac ggcgaatccg caggcagggg
      721 cggaggtaag cgcatcgtga agaagaggcc ggggtcccag cagcagttgg cggcagttgc
      781 gttgtctttt ctgatggttg cgagcggact tgcgcagacg gcgcagcaac cggcaccatc
      841 ctctgtgccc gatgctccga gtgcgacgca gcagcatgcg ccgctcagca ttgctcaaca
      901 ggcccagcag gcgcaacagg tgacgccagt gggacagcaa gctccgtttc acgtagaggt
      961 gccgcactcg cacaatccgt ttgcaccgta tatgccgagc acggtgccac cgatcaatct
     1021 tacaaattca ccgcgtctgc agagtcttgt acgtgacggc aagctttacc tgtctctgca
     1081 tgatgcaatc gcgctggcgc ttgagaacaa tctggacctc gcttattttc gttacaatct
     1141 gccgattgcc caggccgacc tgctgcgtac gaaggccggt ggatctgcaa atggcgtgaa
     1201 taccgccatc gcgcagggga cacagggtgg tttcagcgcg tctggagtgg gcaatggagg
     1261 cggaggatcg agcagtgggg ccacggcggc gggcgcgggc ggactggtga cgtcaactct
     1321 ggggaatggc gcgtctattc cttccttcga tccacatctt aacgtgcagg gctttgtgga
     1381 ccatacgacg ctggtgcagg tgaatacgat ccagtatgga gtgccgattt taaaacaaaa
     1441 cacgatccag ctccaatcca gctactcaca ggcgttttca ctgggcacga attttacgat
     1501 tacggactat gggcttcggc agacgaccaa tagcctttac aacattctga gtcctcagct
     1561 gacgacaaac ttcaacctgg tgattgcgca gccactgctc cagggatttg ggctggcaac
     1621 aaaccagcgc ttcatgcata ttgcgaaaaa gaacattcaa ctgaccgatc tggggttcag
     1681 ggcgcaggtg atcgcaacga ttactcaggt ggaaaacatc tactgggacc tggtgagcgc
     1741 gtacaaagat gcacaagtga aagagcgctc gcttgcctat gccaatgaga cgctttccga
     1801 tgaccagaag cagctgcaat tgcaggcgat tcctgcgatg caggtgatga aggaccaggc
     1861 ggcagtggcg actgcggaag gtgatcttac ggttgcgcgg gcaacgctga aactcaatga
     1921 gctgctgatc aagaatgcgc ttaccaagac gattgatgac ccggcgctgg ccgatatgcc
     1981 tgtgattccg ctggatctta caggcacgtc ggatgcgaat gcggagaagc ccatcgagca
     2041 gcttataacg gaagccgaga agaaccgtcc tgatgtagct gaagaccaga ttgcgatgca
     2101 gattgcgcag aacaacctga agaccatcaa gaatgagctt ctgcccaggc tttcacttta
     2161 tgggcaattc gtcggtgcgg gtttcggcgg tcagctcaac cgctattgtg cgctcgggtc
     2221 ggaatgtaat acgactcttc ctaaggattt tgccggggca tttgaaaaca ccttcaatta
     2281 ctcgtcgcct gaatatcagg tggggttttc tcttgatatc acgttgcgaa accggattgc
     2341 caaggcggac cagtttcggg ccgtgctcga ttaccggcag aaagagctga cgtttgagca
     2401 gcagaagaag agcattctgc tggacgtccg gaactcgcag tatgcgctgc agcaggcgca
     2461 ggcgcgggtg gtagcggcgc agaaggcgcg ggacctggcg cagaagacat ttgatatatc
     2521 caaacaggag cagaagctcg gagccatgtc cgcatacgac gtgcttacga gcgagcaggc
     2581 gctggctgta gctgaatcgg cgctggcggt ggcgcagagc gcctttgaaa aggcgaaggt
     2641 tgatattgac cgcgcgaccg gctcgacgct cgaccgcatg ggagtttcga ttgacgatgc
     2701 gaagagcgga gtggtggcgc acatgcaacc gtaaagagcg tggggtctcc catcttgatt
     2761 cgtgcaaaag aagacggaaa aggacgggtg cccgacagag ctgagtttgc gggtagaatc
     2821 taggttcgga tgtctactac atatcaagcc ggcaggctgg aaaccgaggc tgctgcggcg
     2881 cagccttccc gtgttctgat agccgacgat cagccgtcca ttctggatgc actggagcta
     2941 ttgctgactc cggaagggta tcagattgag aaggcacgct cgccgatggc ggtgctgggt
     3001 gcgctgcaat cgggcgcatt tgacgcgctg ctggttgacc tgaattacac gcgcgatacg
     3061 acttcgggca aagaagggct ggacctgctg acgcacatca agtcacttga cagccaggtg
     3121 ccggtgattg tgatgacggc atgggcgaat gtggaggtgg cggtagaggc gatgcggcgg
     3181 ggggcgcgcg acttcatcca aaagccctgg gacaatgcgc gtctgctgac gatcctgcgc
     3241 acccaaattg agctgcacca tgcagtgagg cgggcacagc atcttgaagc ggaaaaccag
     3301 atgctgcgtg ggcaggggct tccggagttg attgctacgg cgccggcgat gcagcctgtg
     3361 ctgagcacga tccggcgcat tgggccatcg gacgccaatg tgctgattac gggcgagcat
     3421 ggcacaggca aagaagtcgt ggcgcagatg ctccatgcat tgtcaccgcg cgctgcgcgt
     3481 tcgcttgtag ctgtgaatac aggcgcgctg ccggaaggca cgtttgagag cgagcttttt
     3541 gggcatgtaa agggagcgtt tacagatgcg cgaacggagc gcatcggacg atttgaactt
     3601 gccgatggcg gcacgctttt tctggatgaa atcgcaaatg taccgctgcg ccagcaggcg
     3661 aagctgctgc gcgttctgga gaccggagag atggagcgcg ttggctcgtc aaagacgcgg
     3721 cgcgtgaatg tgcgcgtact ctcggcaacg aatgccgatc ttcgtgcgga agccgagcaa
     3781 gggcgttttc gtgaggacct tcttttccgg ttgaatacgg tggaaattca tttaccgccg
     3841 ctgcgcgaac ggcgcgaaga tattccttta ctggccgggc attttctgaa ccgctacgcg
     3901 ggacggtatc gcaagccgat ggaggggatt gatccggcgg cggtgcaggt gatgatgcag
     3961 catccgtggc caggaaatgt gcgcgagctt gaccatacaa ttgaacgcgc agtgctgatg
     4021 gcgcgaggca cgaagattga agcgtccgat ctggggctga accaggtgcg tgtcaccgcg
     4081 cagacccttg aagacatgag cctggaagct gtagagagta ttctcatccg caaggcgctg
     4141 gcccgcgcaa gtggcaacgt gagccaggcc gcagaggcgc tcggcctgag ccgcggcgcc
     4201 ttgtaccggc ggatggagaa atatggcatc tgacagaggg cacgcgacga gcaaaattcc
     4261 cgctgaagga tttcggtttg agacgagggt aaaaatcctg attgcctcgc tgtgcctgcc
     4321 tgtctttgtt ctgattgctg tactgctttg gattgagcat tcctcgctgc ctgtcattgt
     4381 gggaacgctg actgcacttg cgctggtggt cctgctgggg gcatccattt ttattgagca
     4441 agtggtgcgt ccgttgcaga cgctgaccaa tgtggtggcg gcgctgcgag aggaggactt
     4501 ttctttccgc gcgcgcaacg ccactccgca tgatgctctg ggcgagctgg cgattgagat
     4561 caatcagctt gcagatacgc tgcaatcgca acggctgggc gcgctggaag ctgtcgcatt
     4621 gttgcgccgc gtgattctgg agatggatgc gcctgttctg gccttcgatg aacaatgcac
     4681 tttgcgcgtg gtgaatccag cggcggagcg ggtgctgcgg ctgaatgcgg cgcgcgatct
     4741 ggggcggaaa gcaggcgacc tggggttgca gcgtctcata gatgaagcgg acgagggcat
     4801 cgtggccatt gaatcgcagg ggcagcaggc acgctggatg gttcgaaaga gcagcttccg
     4861 ccagcgcgga gtgccgcata cgctgcttgt gctgtctgac gtaagcgcgg cgctgcgcga
     4921 agaagaaagg gccgcatggc ggagactgat tcgcgtgctt ggtcatgaga tcagcaactc
     4981 gcttgcgccg atcaagtcta ttgcgggcag tctgcgtcaa cgcatcgggc aggtgaaaga
     5041 tggcaatact gccgattttg agcgcgggct gaatgtgatt gagagcaggg ccgagtcgct
     5101 caaccgcttt gtgcaggcct accggcacct ggcgcagctt cccgcgcccg tgctgcggag
     5161 cgtttcgctt ccattgctgt ttgaccgcgt agctggcctg gaaacgcgct taccgataga
     5221 aattggcgat gtgccgcagg tgaatctgat ggccgatgcc gaccagatgg agcaattgct
     5281 gatcaattta gtgcgcaatg ccgtagaagc tgccataagc gagcatgaag aaggcggcat
     5341 ggcgggcagt ccggcggtgc gaatttccgg aagggttgcg ggaggctttg cttccattct
     5401 gatcgaggac aatggccccg ggctggcgaa cccaggcaat ctttttgtgc cgttctatac
     5461 aaccaagcgc accggcacgg gggtgggtct ggtgctggcg cggcagattg ccgaggcgca
     5521 tggcggttcg ctggaattgc gcaatcgagc gggcacaaag ggatgccagg cggaggtgag
     5581 gattccgctg gttggggagc gcgaccgtgt ggatgaagcg agggcttcag cggcagagca
     5641 tgatggatag gggattcttc ctccctgcgg tcggcagaat ggcggagttt gtgtgtcggt
     5701 tggcagaatg gtgggctata cagtggcagt ccactgactt ccggtaagat attcgcataa
     5761 caaactacag aaatgcaggt cccattcggc aagctcaggg caggccctcc actccgcgcc
     5821 tttggcgctg cggtcgggat gagaattctt atcaggtcaa gaggagaagg aaacgagtga
     5881 gtgaagctgt ttctaaggtg gccatcctgg gttttggaac ggtgggcagt tcggtggcac
     5941 gcattctttg tgatgcaaag ccgcaaggcg tagaactgac gcatgtgttc aaccgcggtg
     6001 tcgcgcgcaa gcgtgtggaa tgggtcccgg cgggtgtgac gtggactgag aacattgaag
     6061 atgtgttctc gtcctctgcg gatgtggtgc tcgagctcgt aggcggtctt gagcctacgg
     6121 gggaatgggt gcggcgcgcg ctggaggcag ggaagtctgt tgttacggcg aacaaaaagc
     6181 tgattgccga gcacgggatt gctctggaca agctggcgcg caggaaaggt gtccacctgt
     6241 attacggtgc ggccgtggcc ggcggagtgc ctgtgattcc cggcatacag caggggcttg
     6301 cgggagacag cattacgcgc atcgaaggca ttctgaatgg gacgtgcaat ttcattctga
     6361 gcaagatgga agagggcgcg gagtttgcgc ctgttcttaa agaagcgcag aagcttgggt
     6421 atgccgaggc tgatccgacc gaggacgtag gcgggtttga cgcgcgcgcg aaactggtga
     6481 ttctgtcgcg gattgcgctg cgtgccgatg tggcggtggg cgatgtctgc tgcaagccca
     6541 ttaccgagat tgcagccata gattttgcct atgcaaggga aatgggctgc actatccggc
     6601 agatatcgcg ggctgaactg aaggacgggc aggtatgggc tgcggtcggt cctatgctgg
     6661 tgccgctggc ttctccgctg gcgtggtcgc ggggaacaga aaatatggtg ctggtgggcg
     6721 gcgcctatgg cggggacgtg gttttttccg gacacggagc aggggggcat ccaacagccg
     6781 tggcggtggt ttctgacctg ctggcggtgg tacatggctc ccggacgatt gatctaccca
     6841 gcaggaagac accggtcagc gctgatctgc tggtgccgca ctatattcgc tttattgtga
     6901 aagaccgtcc gggaattatt gcgcagattg ccggcgcatt agctgagcag gagatcaata
     6961 tccacgccat cctgcaaaag cccggatata agaagaccaa tctgcccttt gtggtgattg
     7021 ccgagccgtg ttccggatca gcgttgaaac gggcccttga aaaaatcggt catctcgact
     7081 gcttgctgga gccgacgctg gacatgccga ttctggacgc ggaaagcctt agctaaggca
     7141 cagcgtccag gcatcttttt gtaacgggtg gattggcgag gccaccattg ctgtgagtat
     7201 aatcgcggtg cgaatgggtg tgtttgcccg gcgtttttct ccttcctctc tcgttcagcg
     7261 ttcatgccct ccgaatcacc catagcgcca ggggagggga cctttatgtc tacacaagcg
     7321 atgtccaggt cagtggaccc ggacaaaatg caggcacttt tgaaccaggc tgttgccgat
     7381 atgggcgcag ccatgcactc ggtgcttctt gtgattgggg acaggctggg actgtatcgc
     7441 gttatggcca gtggagagcc gatgactcct gccgagctgg cacgccgcac ggggacgttc
     7501 gagcgctatg tgcgcgagtg gctgaatgca aatgcagcgg cgaaatatgt ggaatacgat
     7561 gcagcatcgg gaatgtattt catgaccccg gaacaggcgc agttgctttc ccagggcggg
     7621 atggcgatgg acctgccggg gctgtatcac ttcattgcat cgtgcatgaa ggacgaggag
     7681 aagattaccg aggtttttcg gacaggcaaa ggctttggat ggcacgagca tgaaaagggg
     7741 ctgtttgagg gcacggaacg gttcttccga ccgaattatc tggcaaacct gacgacgaac
     7801 tggataccag cgctggatgg agtggaggca aagctgcgtc aaggggcgcg cgtggccgac
     7861 atcggctgcg gacatggtgc ttcgacgatt ctgatggcac agacttatcc taagtcagaa
     7921 ttttttggct ttgattatca cgaggcatcc attgagcggg cgcgaaagac ggctgccgag
     7981 gccggattat cagaccgtgt gaagtttgag gttgctccgg cgcaggcgta cccgggcgag
     8041 aattatgact ttgccgcatg tttcgattgt ctgcacgata tgggcgatcc tgcgggagcg
     8101 gcgcggcatg tcttgcagtc tctggcgaaa gacggaacgt ggatgattgt ggaaccgcgc
     8161 gcaggcgatg atacggtcgc caatctgaac ccggtgggga agattttcta ttcggcatcc
     8221 actatttgct gcgtgcccgc gtcactgtcg caggaagtgg gcgctggtct gggagcgcag
     8281 gcgggtgaga agaagatccg cgaagtagtg atgcagggtg gatttacgca cttcaggaga
     8341 gcggcagaga cgccgttcaa tatggtattt gaggcacggc gataagacct tatctatcaa
     8401 ggaattcgac tatacgaagc gaagagtggg ctggagtagc tgcgcgcaac tttttggcgt
     8461 tccagggttc taatagatag acagggtctc tgcaagacct taaagggttt tgcagtcacg
     8521 ccagaggaat tgctcttctg gcggggaggt cttcggtgcg tatcctgcgt tcagtcacgt
     8581 tgcttgcggc acttgccttg ccggcgtcgt ttgcccacgg tcagattgct gtgggaatcg
     8641 ggattggccc ggtaattgat tatcctgcac ctgtgtatgt ggcggcgccg cctgtctgtc
     8701 cgtggggata ctatccatac tggccgtatg agtgcgctcc ctatgggtat tacggcccgg
     8761 attggtttgt gagcggagtt tttatcggcg ccggtccgtg gtttacaggt tggttcggag
     8821 cgccgtgggg ctggggctgg ggtcatccct ggggcggctg gggctgggga catccgtact
     8881 ggggctacgg tcgcggttat ggatggggtt ataccagcgg ctactcgcat ggatatgtga
     8941 acggattcaa caatggttac cgcaatggcg gagcctgggg cgtgccacag tcacgtgggt
     9001 tcaataacgg cttccgcgga ggcggcgctt atggtcatac gccgttgggc ggcacgcacg
     9061 tgctgaacag cgcgggttat cgcgcaccat ataatggcgg tttccgcggc agcaatgggt
     9121 tccacacatt taccgggtct acggggttcc acggtaccgg atccacgggg ttccacggat
     9181 atagtggcag ctctggtttc cacggatata gtggcggcgg tatgtccggc ttccatggca
     9241 gttccggcat gcatggcttt ggtgggggcg gcttccatgg cggcggattt ggtggcggag
     9301 gtttccacgg cggcggtgga ttccatggtg gcgggggaca tcgctaaaga aagccgcacc
     9361 tttcgaaacc tggacttgag tagagcttct tacggcattt tcacggattc tgcactccat
     9421 ttgcaatgtg tagtgtcgcc gactccctgt tggtcgcggc gactatagtc tccggacatg
     9481 gtacaggccg tctgtgaaat gctatcgcga acagacacaa ggacgtggaa agtcctgacg
     9541 aagccggata cggaattaca tgacgcggcc gtttcctgaa gcgacgcggt tggcgtgcgc
     9601 accagcgcgc agttttggaa gcatgactcg ataaaagtca cacagcatcc gagtgcgttc
     9661 tgcatcggat cgcaggacga gcagttgctg tttgaagccg agatcggcgg gcagtgctgc
     9721 cgcgagatga aaggcaatgg gcgcgccgag gtcaagttgg acagagggct gctcaatgcc
     9781 ggcaagctcc aggatttcaa aatgcagagc gaggcactct
//
LOCUS       DASRBV010000259         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_270965, whole genome shotgun sequence.
ACCESSION   DASRBV010000259 DASRBV010000000
VERSION     DASRBV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270965"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..896
                     /locus_tag="VFA02_09515"
     CDS             96..896
                     /locus_tag="VFA02_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZU10132.1"
                     /translation="MQIVGCDFHPGQQQVAILDTSTGEVREQKLSHAVKEEVERFYGE
                     LDKPVLVGMEAVGNSLWFEQLLERQGHELWIGDAARIRASEVRRQKTDKRDASHILEL
                     LVTGRFPRIWVPCRQERDQRQLLLHRHKLVGMRTRVKNELQHLALNQGVQKKHSLWSA
                     AGRTELEKLPLEGWTARRRADLLGLLRMLDRQVEALDQAVAAVAAENPQARLLMRQPG
                     VGPITALHPRKHRPLSGDPGTGFCADSGGHNTFSAEQAGGQLSGIDTQ"
     gene            829..1002
                     /locus_tag="VFA02_09520"
                     /pseudo
     CDS             829..1002
                     /locus_tag="VFA02_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011042991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1707..>2127)
                     /locus_tag="VFA02_09525"
     CDS             complement(1707..>2127)
                     /locus_tag="VFA02_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HZU10133.1"
                     /translation="SIDLGNGSYARIIAGELSGTKGPALTFTPVNVFDVRLKAGTKAE
                     LQVPQEHNTGIVLLKGDVVINGVDSVQGEAKIAVLGQEGTVVSLEAKEETVLLVLSGE
                     PIHEPVFSYGPFVMNTRDEIMQAVQDYQDGKMGHLHG"
BASE COUNT          468 a          558 c          671 g          430 t
ORIGIN      
        1 tcggtcgttg attgaataaa ccgagaggct cctgagggat ctcgggtaag ctttgggttg
       61 gagaccgaag cgtgccacgg gagcaaagga gccctatgca gattgtagga tgtgattttc
      121 atcccggcca gcagcaagtg gcgattctgg acacttcgac cggggaggtg cgggaacaga
      181 agctgagtca tgcggtgaaa gaggaagtag aacgtttcta cggggagttg gacaagccgg
      241 tgctggtggg catggaagcg gtgggcaact cgctgtggtt tgagcaactg ctggagcggc
      301 aggggcatga gttgtggatc ggagacgcag cccgcatccg ggccagcgaa gtgcgacgac
      361 agaagacgga caagcgtgac gcgagtcata ttctggagtt gctggtgacg ggacgctttc
      421 ccaggatatg ggtcccttgc cgtcaggagc gggatcagcg gcagttgctg ctgcatcgcc
      481 acaagctggt ggggatgcgg acgcgggtga agaacgagct gcagcacctg gcgttgaacc
      541 agggagtgca gaagaagcat tctttatgga gcgctgcggg aaggacagag ctggaaaagc
      601 ttcccttaga aggatggaca gcgagacgtc gtgccgatct gctggggctg ctcaggatgc
      661 tggacaggca ggtagaagca ctggaccagg cggtggccgc cgtggcagca gagaacccac
      721 aggcgcggct gctgatgagg cagcctgggg tgggcccgat cacggcactc caccccagaa
      781 agcaccgacc gctttctggg gaccccggca ctggcttttg tgctgactct gggggacata
      841 acacgttttc cgcggagcaa gcaggtggcc agctatctgg gattgatacc cagtgagcgc
      901 agttcggggg cgaagcggcg gctgggggcg atttccaagc agggcaacca gtttctgcgc
      961 accctgctgg tggaagcggc gcaatccgct gtcaagcacg atgagcagtt tcggaaagag
     1021 tatcgccgtc gctgccacag caagccgaaa ggcgtggcca aggttgcggc ggcgcgcaaa
     1081 ttggcggtgc ggctgtactg gatgctgaag accgagacac cgtaccgcga tgaagttcac
     1141 accgagggtg gcccgagtca ttctgtggtc ggcaaaagcc agaccgtttc gttgcatggg
     1201 cgctctcgca tcccgtagcc gacgggatgt tcgcacaaac aatcatggcc gcagtacgag
     1261 gccgtatcga tggctggtgg aaccgatgtt ccgccgtgga tgattggcag cagaagagga
     1321 ctcgcgtgac gcttcggtct ccaaaccttc gccacctgag ttgctccgaa gcagctccca
     1381 gcccttctct gccttgacaa cgcctctcgg tttatcgaca gaatgacgga ctctcgtata
     1441 tggaaaatgc gtcaaaaccg gattttctgg cagcggggaa aagctcagcg gtattggtta
     1501 aaccctctta tcgctgagat tcttcctccc ttcggccgtc ggaatgacag atacgtatat
     1561 gtctgtcgct agagtgacag actctacatt gttcggccaa tgtgctgacc gaggccccgg
     1621 aaaccccgtc agcacatgga aaaaatgtta taaaaaaggg cttttttaga aaaaatgctg
     1681 taaaaaagcc gcatttttct tcgttctcag ccgtgcagat ggcccatttt gccgtcctga
     1741 tagtcctgca cggcctgcat gatttcgtcg cgcgtattca tgacgaacgg accgtagcta
     1801 aaaacaggtt catggatagg ctcgccgctc aggaccagaa ggaccgtctc ctccttcgct
     1861 tcgagcgaaa ccaccgtgcc ttcctggccc aacacagcaa tctttgcctc gccctggacg
     1921 gaatccacgc cattgatcac aacgtcgccc ttgagcaaca cgatgcctgt attgtgttcc
     1981 tgcggaacct gcaattcggc ctttgttccg gccttcaggc gaacgtcaaa gacatttacc
     2041 ggagtgaagg tcagcgccgg tccctttgta ccggacagtt cgcctgcgat gatgcgcgca
     2101 taggagccgt taccgaggtc tatggaa
//
LOCUS       DASRBV010000260         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_271004, whole genome shotgun sequence.
ACCESSION   DASRBV010000260 DASRBV010000000
VERSION     DASRBV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271004"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFA02_09530"
     CDS             complement(<1..1316)
                     /locus_tag="VFA02_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU10134.1"
                     /translation="MKHALLASGAAFLLLLSGCMVGPKYVKPDLPAPPSFKEAANAPQ
                     PGGGWKIAQPGDQTARGNWWEIFGDPQLNALEEQVDASNQTLKIAEANFRQARAAIRF
                     NRASEAPAIGVSPGISTVRDSAHQPYFPQSQANNGVGDFTLPVDLSWEIDLWGRIRRS
                     VTAAREQAQASAADMAAVRLSLHSELAIDYFELRSADAQKKLLDDTVKAYTEALRLTE
                     NRFEGGAAPKSDVEQARTQLEQTQVLDSDVSIQRAQYEHAIAILIGKPPASFSLPPVP
                     KDANGPVLPAIPTVMPSELLERRPDIAAAERRMAAANQQIGIAQAAYYPTLSLSALAG
                     FQGTSAMNWLNWPSRFWAVGPTLSETLFDAGRRRATKESALAGYDATVANYRQTALSA
                     FQEVEDNLAALRILSSEAQQQHNATSAAEETLRLFQNRYEGGVDTYL"
     gene            complement(1313..2548)
                     /locus_tag="VFA02_09535"
     CDS             complement(1313..2548)
                     /locus_tag="VFA02_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10135.1"
                     /translation="MNNGEASMVMDGPVETQPETAQTQPPAPAPQPRKSRAVMWIVAV
                     VLCVAIGIMVRSGIHSRVEAEAELTRATQQAALPVVAVVHPRPGSAAEEIALPGNTQA
                     FIDTPVYARTSGYLKKWYVDIGTRVHKGQLLAEIETPELDQQLAQAQAELKNAQANLQ
                     LAQITAARWKHLLETNSVSRQETDQAVSDLNSKQALVASNEANVRRLQELQSFEHVYA
                     PFDGVITARNVDIGALIDAGQNTTPKELFHIASIQKLRVYIPVPEVYAASVKTGEKAT
                     LTLDEFPGQTFTGTITRSSQAIDPVSRTLNVEVDVDNPRGQLLPGAYAFVHLKVPPAE
                     GAVTVPANALIFRKEGMRVAVVRNGKVQMVPIAIGHDYGDTVEVVAGLSPTDAIVVDP
                     SDSLENGAAVQIAAKGAAQ"
     gene            complement(2545..5724)
                     /locus_tag="VFA02_09540"
     CDS             complement(2545..5724)
                     /locus_tag="VFA02_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU10136.1"
                     /translation="MWIVRVALSRPYTFIILALLILILSPVVILRTPTDIFPNIDIPV
                     VSVAWTYTGLNPEELEGRLTTPYEKVLTTLVDNIEHIESTTYNGTAVVKIFLQPNASL
                     DTANAQVTAASQFILRQLPPGTLPPEIINFSASSVPILQIGLSGNGLSEQQLNDYGLN
                     FIRTQMVTVPGAVVPYPYGGKQRQVMINLNQGLMQARGVAPIDVLNAISAQNLVLPSG
                     TAKIGETEYDVRLNTAPRTIAELNDVPIKKAGNTIVYLRDVATVSDGFAVQTNVVRQD
                     GHRGVLISILKAGNASTLDVVKGIRGMLPRIATIVPPELRMAPLADQSVFVRNAVSGV
                     IREAVIAAALTGLMILLFLGSWRSTLIIAVSIPLSILTSIIVLSFLGQTINIMTLGGL
                     ALAVGILVDDATVTIENIERYLEQGLPLERSILEGAAQIAVPALVSTLCICIVFLPMF
                     MLGGVARYLFVPLAEAVVFAMLASYILSRTLVPTLAMYLLKEHDDGPHAPRNVLARFQ
                     RGFEHGFEKLRLGYRALLTRLVENRRLFVPSFIGTCLCAFLLVPWLGQDFFPNTDSGE
                     FILHVRGKTGLRIEETARLCDLVEDSIRQEIPAKEIDHIIDNIGMPYSQMNTMHMTSG
                     TIGANDADIMVTLREDHHPTANYIRRLRQDLPAQFPGTSFYLLPADIVTQILNFGLPA
                     PMDIQIEGNNIEASHEIANKLLIQLRQVPGLTDLRIQQPFDYPILNVNVDRTKAQQGG
                     YTEHDVASSVLNTLSGSFQITPMFFLNWKNGVNYNLVAQTPQYHIQSLQDLQNIPMTA
                     AGTQQPEILGDVASIDRSREMAVISHYNIRRVVDIYGSVQDRDLGAVSREVEHIVDAN
                     KKDLPRGTFVSVRGQVQTMRASYTGLLTGLLFAIVLVYMLIVVNFQSWLDPFIIITAL
                     PAALAGIVLFLFFTHTTLSVPALMGAIMCMGVATANSILVVSFSKERLQVHGDAVRAA
                     IEAGATRFRPVLMTALAMIIGMVPMALGLGDGGEQNAPLGRAVIGGLLCATVATLVFV
                     PSVFSMMHGRRHKENAEVSLEQEGHATHV"
     gene            5868..>6347
                     /locus_tag="VFA02_09545"
     CDS             5868..>6347
                     /locus_tag="VFA02_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU10137.1"
                     /translation="MELRHLRYFCAVAEWRGFNRAARALHVSQSAISEQVLDLENEIG
                     VALLNRSQHRVSLTPAGEVFFNEARKVLAAADRAVDLAQRSARGESGALTIGFLVWGT
                     GRFFPTIIREFRRQHPHIRLSVMEMAPAAQSEALLNGSIDIGFTRPLQPPYDGLLQSE
                     "
BASE COUNT         1384 a         1704 c         1850 g         1409 t
ORIGIN      
        1 agataagtgt ccacaccacc ttcataacgg ttctggaaga gccggagggt ttcttcggcg
       61 gcgctggttg cgttgtgctg ttgctgggcc tcggaactca gaatgcgcaa tgccgccaga
      121 ttgtcttcca cctcctggaa ggcggagagc gccgtctggc gatagttcgc aactgttgca
      181 tcatagcctg cgagggcaga ttctttggtc gcgcgacggc ggcccgcatc aaaaagcgtt
      241 tctgacagag ttgggcccac tgcccagaag cggcttggcc aattcagcca attcatggcg
      301 gatgtgccct gaaaccctgc cagcgcactt aggctgagtg tcgggtaata tgctgcctgc
      361 gcaattccga tttgctggtt ggccgcggcc atgcgccgct ctgccgcggc aatgtccgga
      421 cggcgttcca gcagctctga aggcataacg gttgggatgg ccggcaacac ggggccgtta
      481 gcgtccttgg gcacaggcgg cagactgaag gatgccggtg gtttgcctat gaggatggcg
      541 atggcgtgct catattgcgc ccgctgaatg gaaacatcag aatccagcac ctgcgtctgc
      601 tccagttgcg tgcgtgcctg ttctacatca gatttcggag cagctccgcc ctcaaagcgg
      661 ttttcggtga gccgcagggc ctctgtatat gcctttactg tgtcgtcgag cagctttttc
      721 tgagcatcgg cgctacgcag ctcgaagtaa tctatggcca gctcagaatg caggctcaac
      781 cggacggcag ccatatctgc ggcgcttgcc tgggcctgct ctctggcagc agtcacgctg
      841 cggcggatgc gtccccagag gtcaatttcc caggaaagat ctacgggcag ggtgaaatca
      901 ccgacgccat tgttggcctg tgattgcggg aagtatggct gatgcgccga atcacgcacg
      961 gtggaaatgc ccggagatac gccaatggcc ggcgcttctg atgcgcgatt gaaacgaata
     1021 gcagcgcgcg cctgacggaa gttggcctcg gcgatcttca gcgtctggtt ggacgcatct
     1081 acctgttctt caagcgcatt cagttggggg tctccgaaga tttcccacca gttcccgcgg
     1141 gcagtctggt cgcctggttg cgcgatcttc catccgccgc caggctgcgg cgcattggcc
     1201 gcttctttga aagagggcgg agcaggcaga tcaggcttca catattttgg accgaccata
     1261 cagccggaaa gcagcaggag aaatgccgct ccagatgcca gcagggcgtg cttcattgcg
     1321 ccgccccttt tgcagcaatc tgcacggccg ctccgttttc cagcgaatcg gaggggtcca
     1381 cgacaatcgc atctgttgga gaaagtccgg cgacaacttc tacggtgtca ccatagtcgt
     1441 ggccaattgc gatagggacc atctgcactt ttccatttct cacaactgca acacgcatgc
     1501 cttctttacg gaagataagc gcattggcgg gaacggtgac ggcgccctca gcaggaggca
     1561 ctttgagatg cacgaaggcg taagctccgg gaagaagctg gcccctgggg ttgtccacgt
     1621 ctacttcaac attcaatgtt cgagacacag gatcaatcgc ctgagagctt cgtgtgattg
     1681 tgcctgtaaa cgtctgtccg gggaattcat ccagcgtaag agtggccttt tcgcctgttt
     1741 ttacggatgc ggcgtacact tccgggaccg gtatgtagac gcgcagcttc tgaatagaag
     1801 cgatgtgaaa caactctttc ggagttgtat tctggccggc atcaatcagc gcgccgatgt
     1861 ccacattgcg ggcggtaatg actccatcaa acggagcata cacgtgttcg aaggactgaa
     1921 gctcctgcag gcgtcgcaca tttgcttcgt ttgaagccac gagcgcctgc tttgaattca
     1981 agtcgcttac ggcctggtct gtttcctgcc tggaaacgga gttggtttcc agcagatgct
     2041 tccagcgggc cgcggtgatt tgtgcaagct gtagattggc ctgagcattt ttcaactccg
     2101 cctgtgcctg cgccagttgc tggtcaagct cgggcgtttc gatttctgcc aggagctggc
     2161 ctttgtggac gcgggtcccg atatctacgt accacttttt cagatagccg ctggtgcggg
     2221 catagaccgg cgtgtcaatg aaggcctgcg tatttccggg cagagcaatc tcttcggcag
     2281 cggagcctgg ccggggatga acaactgcca ctacaggaag agcggcctgc tgtgttgcgc
     2341 gcgttaattc agcctcggct tccacacgcg agtggatgcc cgaacggacc atgatgccga
     2401 tggcaacgca cagcacaaca gccacgatcc acatgacggc gcgagacttc cgcggctgcg
     2461 gggctggagc aggcggttgc gtctgagcgg tttcaggctg tgtttctacc ggaccatcca
     2521 tcaccatgga agcttctccg ttattcatac gtgtgtcgcg tgtccttctt gttcgagcga
     2581 tacttctgcg ttctctttgt gtctgcgtcc gtgcatcatg ctgaagactg acggaacgaa
     2641 gaccagtgtg gccacggtgg cgcaaagcag tccgccaatg acagcgcggc ccagaggagc
     2701 attctgctca ccgccatcgc ccagccccag cgccatgggc accataccga taatcatcgc
     2761 cagcgcggtc atcagtaccg gacgaaaacg cgtcgcgcct gcttcaatgg cagcgcgtac
     2821 cgcatcgcca tggacttgca atctctcttt tgagaaagat acgacaagga tactgttcgc
     2881 agttgccacg cccatgcaca tgatggcgcc cataagagca ggaacactca gcgttgtgtg
     2941 cgtaaagaaa aggaacagca caattcctgc caaggcggcc ggcaatgctg tgatgatgat
     3001 gaacggatcc agccacgact ggaagttcac gacgatcagc atatagacca gcacaatggc
     3061 gaagagcagg ccggtgagca gtcccgtgta agaagcgcgc atggtctgta cctgtccgcg
     3121 cacggaaaca aaggtgccgc gtggcaggtc tttcttattg gcatctacta tatgttcaac
     3181 ttctcggctt actgcgccga ggtcgcggtc ctgcacggaa ccgtagatgt ctaccacacg
     3241 gcggatgttg tagtgtgaga tgacggccat ttcccgtgac ctgtcaatgg aagctacatc
     3301 acccaggatt tccggttgct gcgtgccggc cgcagtcatc ggaatgttct gtaaatcctg
     3361 aagcgactgg atgtgatatt gcggcgtctg cgccaccaga ttgtagttca caccattttt
     3421 ccaattgagg aagaacatcg gcgtgatctg gaagctgccg ctcagtgtgt tgagcacgct
     3481 cgaagcgacg tcgtgttcgg tgtatcctcc ctgctgggcc ttcgtgcggt ctacattgac
     3541 gttgagaatc gggtaatcga agggctgctg gatgcggagg tcagtcaggc cggggacctg
     3601 gcgcaactgg atcagcagct tatttgcaat ttcatggctg gcctcgatgt tattgccttc
     3661 aatctgtatg tccatcggcg caggcaggcc aaaattcaga atctgcgtca cgatgtcagc
     3721 cggaagcaga taaaacgaag tcccggggaa ctgtgccggt aggtcctgcc gcagccgccg
     3781 aatgtaattt gccgtaggat gatgatcttc cctgagagtc accataatgt cggcatcgtt
     3841 tgcaccaatg gtgcctgacg tcatatgcat ggtgttcatc tggctgtaag gcatgcctat
     3901 gttgtcaatg atgtggtcta tttcttttgc aggtatctcc tggcgaatgc tgtcttctac
     3961 aagatcgcag aggcgggccg tttcctcgat gcgaagaccg gttttgccgc gcacatgcag
     4021 aatgaattct ccgctgtcgg tgttggggaa gaagtcctga ccaagccatg gcaccagaag
     4081 aaaggcgcaa aggcatgtgc caatgaatga aggcacaaag agccttcgat tttctaccag
     4141 acgtgtgaga agcgcacgat agcccaggcg cagcttctca aatccatgct caaaaccgcg
     4201 ctgaaatcgc gccaggacat tgcgtggagc gtgcggccca tcatcgtgct ctttcagcag
     4261 atacatggca agcgtcggca ccagcgtcct tgaaagaatg taagaggcaa gcatggcgaa
     4321 gacgacggcc tcagcgagcg gaacaaacag atagcgcgcc actccgccga gcataaacat
     4381 aggcagaaag acgatgcaga tgcacaaggt agagaccagg gcgggaacag caatctgcgc
     4441 ggcaccttca agaatggaac gttccagcgg aagcccttgt tcgagataac gctcgatgtt
     4501 ctcaatggtg acagtggcgt cgtccaccag aatgcctacg gcaagcgcga ggccacccag
     4561 cgtcatgatg ttgatggtct gcccgagaaa gctgaggacg atgatcgagg tcagaatcga
     4621 aagcggaatg gacaccgcga tgatgagtgt gctgcgccag ctaccgagaa agagaagaat
     4681 catcagtccc gtcagtgcag ctgcaatcac cgcttcacga atcacgccgc tcacagcatt
     4741 gcgaacaaaa accgactggt cggcaagcgg ggccatacgc agttccggcg gtacgatcgt
     4801 tgcgatgcgc ggcagcatac cgcgaatgcc cttaaccaca tccagcgtag aagcattgcc
     4861 ggccttaaga atgctgataa gcacgccacg atggccgtcc tggcggacaa cgttggtctg
     4921 cacggcgaat ccatcgctga ccgttgccac gtcgcgcaga taaacgatgg tattcccagc
     4981 tttcttaatg ggaacatcgt tcagttcagc gatggtacgt ggcgctgtat tcaggcgaac
     5041 atcgtactct gtctctccga tctttgcggt accagaaggc agcaccagat tctgcgcact
     5101 aatggcattg aggacatcta tcggagcaac gccgcgcgcc tgcatcagtc cctgattcag
     5161 gttgatcatg acctggcgct gttttcctcc gtaggggtag ggaactactg cgcctgggac
     5221 agtgaccatc tgtgtcctga tgaagttcag gccatagtca ttgagctgct gttcagaaag
     5281 gccgtttccg gaaagcccga tttgaagaat gggcacactc gaagcgctga aattaataat
     5341 ttcaggaggc agcgtgccgg ggggaagctg acgaagaatg aactgcgaag ccgctgtgac
     5401 ctgcgcgttt gccgtatcga ggctcgcatt tggctgaaga aagattttta ctaccgctgt
     5461 gccgttgtat gtggtggatt cgatgtgctc aatgttatct accaaagtgg tgagcacttt
     5521 ttcgtacggc gttgtgagcc ggccttccag ttcttcagga ttcaaccccg tataggtcca
     5581 tgcgacggaa acgacaggaa tatcaatgtt tggaaagatg tcagtcggcg tgcggagaat
     5641 gaccaccggg ctcaggataa gaatgagcag cgccagaatg atgaacgtat aaggccgcga
     5701 tagcgcaact ctgacaatcc acataaatga ccgcttggct ccggggaaag atgtcgaatg
     5761 gacgtgctcc gcacacgcca gacattcttt ttgaggaact tggctgctga taggtcgcac
     5821 agtttgttcc gaatgcaaca atcggtaatg gcgataagat agaagtcatg gaattgcggc
     5881 atcttcgtta cttttgcgct gttgcagaat ggcgtggttt taatcgtgca gcgcgcgccc
     5941 tgcatgtctc gcagtcggcg atcagtgagc aggtgctcga tcttgaaaac gagatcggtg
     6001 tcgccctgct caaccggtca cagcatcgcg taagccttac tccggcggga gaagtgtttt
     6061 tcaacgaggc gcgcaaggtg cttgcggcag cagatcgagc tgtagacctg gcgcagcgtt
     6121 ccgcgcgcgg agaatcaggc gcgcttacca ttggcttttt ggtatggggc acaggcaggt
     6181 tctttccgac catcatccgg gagtttcgcc gtcagcaccc tcatatacgg ctttcagtca
     6241 tggaaatggc gcccgcggca caatcagagg cgctgctgaa tggctcgatt gatatcggct
     6301 ttacgcgacc tctgcagcca ccctatgatg ggcttcttca atcagaa
//
LOCUS       DASRBV010000261        10978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_223590, whole genome shotgun sequence.
ACCESSION   DASRBV010000261 DASRBV010000000
VERSION     DASRBV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223590"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1014)
                     /locus_tag="VFA02_09550"
     CDS             complement(208..1014)
                     /locus_tag="VFA02_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU10138.1"
                     /translation="MFYTSDGTRLFYEIMGEGEDVFLLHPTPVHHAFWIPVAEILAQK
                     YRVILPDLRGHGQSEAGEAPITIPKLGSDIIKLLDELGIRKALFAGCSVGGYTLYELW
                     RRIPERIRALAFCCSKPQADTETGRLKRHEWMDNVRLRGTGDFFEQMLQMLIGPTMWR
                     RFPQKIGEAREMMQTMSADAVIAMQQGLAARPDSVATAYTISVPCCVIAAAEDPGSTV
                     ADMKLLAETIRNGGYGAEFHVLHDAGHFAPWEQPETVGPVLRRFFDSVAG"
     gene            complement(1024..1356)
                     /locus_tag="VFA02_09555"
     CDS             complement(1024..1356)
                     /locus_tag="VFA02_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZU10139.1"
                     /translation="MAVFCSNCGKTLGDDARYCSGCGKPLYAAPPPFVQTRLVRPRAG
                     RMVAGVCQGLANNYHWDVVWVRVITVLATIFSSGAGLIAYIVFWLVMPEEPLALPPSQ
                     TYTQPTGS"
     gene            complement(1492..1806)
                     /locus_tag="VFA02_09560"
     CDS             complement(1492..1806)
                     /locus_tag="VFA02_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10140.1"
                     /translation="MALKEDMPFAAEVPSNSIESTGTMSGDGTRQLLHSGAWTLAAGL
                     LAVVLAKFVFGGIGPEGPHTNSGWLALIVALMCLPFGSLLLLLGIAKWLRNRAQNSEH
                     KS"
     gene            complement(1797..2342)
                     /locus_tag="VFA02_09565"
     CDS             complement(1797..2342)
                     /locus_tag="VFA02_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZU10141.1"
                     /translation="MQSLETATPPAKAPIAALILISAAASAFLFWLIYIHPPAQEYAT
                     RLLFLPALNALFNGMSAIALAIGFSCIRKKNIAAHRAWMLTAFAFSVLFLVSYTINHA
                     LHGEMRFAGHGPVRTAYLALLVSHILLSVIALPMVLITFFFSLTGRFPQHRKIAPYTF
                     PIWMYVSVTGVVVYVMLTAWR"
     gene            complement(2408..3238)
                     /locus_tag="VFA02_09570"
     CDS             complement(2408..3238)
                     /locus_tag="VFA02_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10142.1"
                     /translation="MATMVATTSPPRALPSRNASLWTAALTLWEREIVRFYRQKARVI
                     GVIASPLLFWFVLGSGFAHSFRGGATGSDQYLSYFFPGAVVMIVLFTAIFSMMSLIQD
                     RNEGFLLSVMAAPVSRSAIVLGKVLGGATLAAIQGVVFLVFTPLVGVHPSLTAIGISV
                     LAIVLISFELTALGFAIAWPMDSPQAFHAIVNLLLLPLWMLSGAIFPASGATGWLRAL
                     MRVNPLTYGTDALRNALFPAQTTGFSLSVNLGVTIAFCLLVFAASWTIVDRRTNKPAA
                     "
     gene            complement(3241..4263)
                     /locus_tag="VFA02_09575"
     CDS             complement(3241..4263)
                     /locus_tag="VFA02_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU10143.1"
                     /translation="MASATEKTTAVLKNNPAWMEPITLDGLTHHYGDRLALDHLTFQV
                     RPAEIFGLLGPNGSGKTTLFRILSTLMIPTGGSALIQGFDVAREPNRVRQQIGIVFQA
                     RSLDIKLTVGENLKHQGHLYGLKGGTLKRRMEEVLTRVGLLDRIKDTVETLSGGMQRR
                     VELAKGLIHSPSVLLLDEPSTGLDPGARRDLWQYLRMLRDDEGVTVLVTTHLMEEAEH
                     CDRLAILNQGKLVALGAPSELKSEIGGDVVLFEAASEASAAELSAKIANRFIVSPTVM
                     GNTVRLEREQGHKFVTDVVEAFPGLVEGVSVARPGLEDVFIQRTGHRFWTEKTEQQAW
                     PPQKEQ"
     gene            complement(4267..5241)
                     /gene="cyoE"
                     /locus_tag="VFA02_09580"
     CDS             complement(4267..5241)
                     /gene="cyoE"
                     /locus_tag="VFA02_09580"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZU10144.1"
                     /translation="MTPAAPASVADEPAIAEKPTHLRAGETASRITDYAELFKFRVTA
                     LVVMTAWAGFYLASMRSGISSVQPGLIAALLGIGLVSAGSAALNEALERRLDAKMVRT
                     SQRPMASGRISLPHGLLLGMGAIVAGSLWLTFATNLLTGVLTLLTAFSYVAVYTPLKR
                     YTTLATFIGAFPGAMPPLLGWTAVRGTIEWPAVALFAILFVWQFPHFMAIAWLYKEDY
                     GRAGIRMLPVVQPDGWSTVLEAITYAVLMVPVSLLPVYLHMAGRVYGVTALVLGIFYL
                     AYTLRFARITRARSVVESKMYARDLLKVSVIYLPVLLTVLMLNAAGKQ"
     gene            complement(5344..6411)
                     /locus_tag="VFA02_09585"
     CDS             complement(5344..6411)
                     /locus_tag="VFA02_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZU10145.1"
                     /translation="MSSRTLRSDIVFTFALVIGLALAWYVRNILLLIYVSALFAVVLM
                     PIVQGIMKLRIGKWSPGRGMAILILLLLVAGCTTVFFVFALPPVIHDLREFVSELPTR
                     GPQLLARVRRLPLMQRIDINSLNARLQDFAANFATYLLFSARHWASKLFDVITGIVLT
                     VYFMLEGEKAYHWLLSFFPVDMRQRLDKTLVRAEARMGQWLLGQGVLMLILGTASTIV
                     YVSLHVRYAYALGVLMGIFNIIPIAGAVITVSLSLLVAAIDSWGRVLGVAIFYAIYAQ
                     VETSYLTPRIMQSRVDLPGLAIIVALLLGAAMDGIRGALVAVPTAVLVAVLLDEYAVN
                     NEPVISGPKPLNEVDELHSRT"
     gene            complement(6408..6971)
                     /locus_tag="VFA02_09590"
     CDS             complement(6408..6971)
                     /locus_tag="VFA02_09590"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZU10146.1"
                     /translation="MAITAQLPARISRIVLIGFMGAGKSTVGALLAPQLGWRFLDADT
                     VLEERAGATIAEIFAGRGEPAFRRMEAETIRDLLREHNLVLAVGGGAVETTATREAIF
                     SAPDTCVVFLEAPLETMIARCEQQPGAVIRPVLKDRERLKTRFESRLPHYRNAHLVVE
                     TAARTPQETVRHIVESLSLLFKEGQPA"
     gene            7107..7748
                     /locus_tag="VFA02_09595"
     CDS             7107..7748
                     /locus_tag="VFA02_09595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10147.1"
                     /translation="MRLFRKKLKQEHKVMLQAERAGGALLPEYHRPTPECPNPERWHM
                     YDSMTAEAEVLEFLQVLVTTIKPGLVVETGAFLGASTIWIARGLKANGFGKVVSCEFD
                     PVVFTKAKEKIAASGLAEWIELRNESSLEMQIDGTIDLFFSDSDMPIRSAEVKRFLPQ
                     ISPTGVVLIHDASSHLKIVREAAFELEREGLLACVFLPTPRGLVIAQKREGRK"
     gene            complement(7752..8690)
                     /locus_tag="VFA02_09600"
     CDS             complement(7752..8690)
                     /locus_tag="VFA02_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HZU10148.1"
                     /translation="MAATQNLQSDEEIATFRRFNRLYTRTIGTLSEGLLNSQYSLTEA
                     RVLYELAHGRMAAREIAEELGLDPGYLSRILRKFEDMGLLKKTASTTDARQMQLSLTA
                     KGRTAFDELDQRSNKQACDLLNQLPPSKREALVRSMREMENALSMERPSAPYILRQHR
                     PGDMGWVVSRHGALYAQEYGWDEQFEALVARIVADFIINYDSRRERCWIAERNGDRLG
                     CIFLVKHPEQNETAKLRLLLVEPSARGLGLGKALVNECVSFARTAGYKRMTLWTQSIL
                     AAAHHIYQQAGFRLVHEGPHHSFGVDLIGQTWEMEL"
     gene            complement(8778..10115)
                     /locus_tag="VFA02_09605"
     CDS             complement(8778..10115)
                     /locus_tag="VFA02_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZU10149.1"
                     /translation="MNKRDFLKTSGILVSGATLSHYLPAEQISGTRTNWAGNYTYSTN
                     NLYEPKSVEEVRQTVKRCQKIRALGSRHSFNAIADSKYNQISLSHLEEMAIDQKARTV
                     TVGGGVRYGKLAPYLDSHGYALHNLASLPHITVPGACATATHGSGMHNGNLATAVLAL
                     EFVTADGQIASLSRAKDDDVFEGAVVSLGGLGVITKITLDLLPRFEMRQLVYENLSFD
                     QLRDNLDAIFGSGYSVSLFTDWQDHRATQVWIKQRMDSDISSSLPSDFYGAKLATHKL
                     HPIAGHDATPCTEQMGTPGPWYERLPHFRMNFTPSSGAELQSEYFVPREHSFEAILAV
                     EKLRDRITPHLFITELRTIAADNLWMSMAYQRDSLAIHFTWKPEWPAVKEILPLIEKQ
                     LEPFDPRPHWGKLFTMPPGQLQAKYEKLNDFKGLLKHYDPEGKFRNTFLDTNI"
     gene            10234..10701
                     /locus_tag="VFA02_09610"
     CDS             10234..10701
                     /locus_tag="VFA02_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10150.1"
                     /translation="MKRIFALVLCFVFAISVLAADNGYKIVYDGGSVTAMKAGNKAEL
                     YIDSNQIRLNEDGKDIAVIPASAVTEISYGQDVHRRVGAAIGLAVVSFGLGGLMALTK
                     SKKHYVGLTWSNGDQKGGFAMQCDKNEYRGILAGLEGVTGKKAVNSDTMTVKN"
BASE COUNT         2592 a         2901 c         3187 g         2298 t
ORIGIN      
        1 cgaatcgaac aaaagaaaca gaccagatca tctgcttctt ccgactggcc gacagtaatg
       61 tcatggaaga aggaaacgcg gtcagcagag gcgtgggcgc gcggaacaaa tgtcccggcg
      121 agcagcattg caaaacacaa ataaagcaaa cggcgtgaca tctacccctc ccgtctgcac
      181 tacgcagatt cgtccagccg ggttccctta tccagccaca gaatcaaaga aacgacgcaa
      241 aaccggtcct actgtttccg gctgttccca gggggcaaag tgcccggcat cgtgtaaaac
      301 gtggaactcg gcaccatagc cgccatttct gatggtttcg gccaggagct tcatgtctgc
      361 cacggtgctg ccggggtctt ctgccgcggc gatcacgcag caaggtacgc taatggtata
      421 ggccgttgcc acggaatcag gacgcgcagc aagtccctgc tgcatggcaa ttaccgcatc
      481 ggcagacatg gtctgcatca tttcacgcgc ctcgccgatc ttttgcggga agcgccgcca
      541 catggtcggg ccaatcagca tttgcagcat ctgctcaaag aaatcgcccg tgccgcgcag
      601 ccggacattg tccatccact catgccgttt caggcggccc gtttccgtat cggcttgggg
      661 tttgctgcaa cagaaggcaa gcgcgcggat gcgctccggt atcctgcgcc aaagctcata
      721 aagggtgtat ccgcctacgg agcatccggc aaaaagtgct ttgcgaatgc ccagctcatc
      781 cagcagcttt atgatgtccg agccaagctt tggaatcgtg ataggggctt cgcctgcttc
      841 cgattggccg tgtccgcgca aatcgggcag aatgacgcgg tatttctggg ccagaatttc
      901 ggccaccgga atccagaaag catggtggac tggcgtaggg tgaagcagga agacgtcctc
      961 cccttcgccc atgatttcgt agaaaagccg cgttccgtct gaagtataga acacgggtct
     1021 attttagctg cctgttggct gcgtgtaggt ctgagatgga ggcagcgcaa gcggctcttc
     1081 cggcatgaca agccagaaga cgatataagc aatcaagcca gcgccgcttg agaagatggt
     1141 tgccaaaacg gtgatgacgc gtacccagac tacgtcccag tgataattgt ttgccagccc
     1201 ctggcagacg ccagcgacca tgcgacccgc gcgcgggcgg accagacggg tctgtacaaa
     1261 cggtgggggc gcggcgtaga gcggctttcc gcacccagaa caatagcgcg catcatcgcc
     1321 cagagtctta ccgcagttgc tgcaaaatac agccatacat cactcccttt tcctcaaaac
     1381 tgggatacgc aaaatgccag cttgcggttc cctcgctacc tgcctgctat tttatttcct
     1441 cttgacgggc caggcatatc cgttcagagg cagtattacg ctgcaatcct gctaactctt
     1501 atgttctgag ttttgtgccc tgtttctcaa ccacttggca atccccagaa gaagcaggag
     1561 ggagccaaag ggcaggcaca tcagggccac aatcagggca agccagccgg aattggtgtg
     1621 cgggccttcc gggccgattc cgccaaagac gaattttgcg agcacaaccg ccagcagtcc
     1681 cgcggccagg gtccatgcac ccgagtgaag aagctgacgt gtaccatcgc cggacatggt
     1741 tcctgtgctt tcgatcgaat tcgaggggac ttcagcggca aatggcatat cttctttcag
     1801 cgccatgctg tcagcatgac ataaacaaca actccggtaa cggaaacata catccagatg
     1861 gggaaagtgt acggcgcaat ttttctatgc tgcggaaagc gccccgtcaa tgaaaagaaa
     1921 aatgtaatca gcaccatggg cagggcgatc acagaaagca gaatgtgact gacgagcagc
     1981 gccagatagg ctgtccggac agggccatgt ccggcaaagc gcatttctcc gtgcagtgca
     2041 tggttgatgg tgtaagagac cagaaagagc acagaaaaag caaaggccgt cagcatccag
     2101 gccctgtgtg ccgcaatgtt tttcttacga atgcaggaaa atccaatggc gagggcaatg
     2161 gcgctcattc cgttaaacaa cgcgttgaga gcgggcagaa agagcaggcg tgtggcgtat
     2221 tcctgtgccg gcggatgaat atagatcagc cagaagagga aagcgctggc cgccgcgctg
     2281 atgagaatca aggccgcgat gggcgctttg gccgggggcg tcgctgtttc caggctctgc
     2341 attgggtctt tctcttttcc ttgttctata tttccgggtg tcccgcatcc tgaaaagatg
     2401 tgggccgtca cgctgccggt ttgtttgtcc tgcggtccac aattgtccag gacgcagcaa
     2461 ataccagcaa acaaaatgcg attgtcacgc ctaaatttac gctcaaagaa aagccggttg
     2521 tctgcgctgg aaacagcgcg ttgcgcagcg catctgtccc gtaagtgagc ggatttacgc
     2581 gcatgagcgc gcgcagccag ccggttgcgc cactggctgg aaagatggcg cctgaaagca
     2641 tccacaacgg gagcagcaga agattcacga tggcatgaaa ggcctgcggt gaatccatcg
     2701 gccaggcgat ggcaaagccg agcgctgtca gttcaaagct gatgaggaca atcgcaagga
     2761 cgctgatgcc gatggcggtc aggctgggat gtacgccgac gagcggagta aagaccagaa
     2821 atacaacccc ctgaatcgca gccagcgttg cgccccccag caccttgccg agtacgatag
     2881 cggagcggct tacaggcgca gccatgacgg agagcagaaa gccttcattg cggtcctgga
     2941 tcagcgacat catgctgaag atggccgtga agagcacgat catgaccaca gcaccgggga
     3001 aaaaatagct gaggtactgg tcagaaccgg tagcgccacc gcgaaagcta tgcgcgaacc
     3061 cggaacccag cacgaaccag aagagcaaag gtgaggcaat gacaccgatg acacgcgcct
     3121 tctggcggta gaagcgaaca atttcgcgtt cccagagcgt caaggccgcc gtccagaggc
     3181 tggcattgcg tgaaggaaga gcacgcggcg gagacgtggt agcaaccata gtagccatgt
     3241 tcattgctcc ttctgagggg gccatgcttg ctgttcagtt ttttccgtcc agaagcgatg
     3301 accggtgcgc tgaataaaga catcttccag gccaggcctg gccaccgaga cgccctctac
     3361 gagtccggga aatgcctcaa caacatctgt gacgaactta tgtccctgct cgcgctcaag
     3421 acggacggta tttcccatta cggtgggcga gacgatgaaa cggttggcga tcttcgcgga
     3481 gagttctgct gccgaggctt cgcttgctgc ctcaaacagc acgacatcgc cgccaatttc
     3541 agacttcaac tcggatggcg ctcccagcgc aaccagtttg ccctggttca aaatggccag
     3601 gcggtcgcag tgctcggcct cttccatcag gtgggttgtg accagcacgg tcacgccttc
     3661 gtcatcgcgc agcatccgca gatattgcca caggtcgcgg cgcgctccgg gatcaagtcc
     3721 ggtggaaggc tcatcgagca gaagtacgga aggggaatga atcaatccct tcgccagctc
     3781 gacacggcgc tgcattccgc ctgagagtgt ttcgaccgtg tccttgattc ggtcgagcaa
     3841 tccaacgcgc gtcagcactt cttccatgcg ccgcttcaaa gtgccgccct tcaagccgta
     3901 aaggtggcct tgatgcttca ggttttcgcc gacggtaagt ttgatatcga ggctgcgcgc
     3961 ctggaagacg atgccaatct gctgtctcac ccggtttggc tcgcgggcga cgtcaaaccc
     4021 ctgaatcaga gctgaaccgc cggtaggtat catcagggtt gagagaatcc ggaagagggt
     4081 cgtcttgccg ctgccattcg ggccgaggag gccgaaaatc tcagccggac gtacctgaaa
     4141 cgtaagatga tcgagggcca gccggtcgcc gtaatgatgg gtaagcccgt ccagtgtaat
     4201 tggttccatc caggccgggt tgttcttaag aacagcggtt gtcttttcgg ttgcagatgc
     4261 cataaattac tgctttcctg ccgcgttcag catcagcaca gtgagcagga cgggcaggta
     4321 gatcacgctc accttcaaca ggtcccgcgc atacattttg gactcgacca cgctgcgcgc
     4381 gcgcgtaatg cgggcgaacc gcagcgtata tgcaagataa aaaatgccaa gaaccagcgc
     4441 ggtcacgcca tacacccggc cagccatatg caggtacacc ggaagcaggc tcacgggcac
     4501 catcagcact gcataagtaa tggcctccag gacggtagac cagccatcgg gctggacaac
     4561 cggcaacata cggattccgg cgcgcccgta atcttcttta tagagccacg caatcgccat
     4621 aaagtgcgga aattgccaga caaaaagaat ggcaaacaga gcgacggcag gccattcaat
     4681 ggtgccgcgg acagcggtcc atcctaagag tggaggcatg gcgccgggga atgcaccgat
     4741 aaatgttgcc agagtggtat atcgcttaag gggagtgtaa accgccacgt agctgaaggc
     4801 ggtaagaagc gtcagcactc cagtgagaag attggtagcg aaggtcagcc agagggaccc
     4861 ggcgacaatt gcgcccatac ccaaaagcag tccatgcgga aggctgatgc ggcctgaagc
     4921 catgggacgc tgcgaggtgc ggaccatctt agcatcaagc ctgcgttcaa gcgcttcatt
     4981 cagggcggcg gagccagcgg agacgagacc gattcccagc agggcagcaa tcagaccggg
     5041 ctgaacgctg ctgattccgc tgcgcatgga tgcgagataa aaccctgccc aggccgtcat
     5101 gacgacgagc gccgtgacgc gaaatttgaa tagttcagcg tagtcggtaa tgcgcgaggc
     5161 ggtctcgcca gcccgcaaat gtgtgggctt ttcggcgatt gccggctcgt cggcaacgga
     5221 cgctggtgcg gcgggcgtca tcaatgtttc gctcgagacg gggtgaattt tgcgggcgac
     5281 accactcttc atgatatcaa gcgaaatctg atgtttgctg gactggccgc catatcccaa
     5341 gatttatgtt cgcgaatgca gttcatctac ctcgttcagc ggtttcgggc ccgagataac
     5401 aggctcatta ttaacggcat actcatccag caggacggcg accagaacgg ccgtgggcac
     5461 ggcaacaaga gcgccccgga taccgtccat ggccgcgccc agaaggagcg ccacgatgat
     5521 ggcgagtccg ggcaggtcta ctcgggactg catgatgcgg ggcgtaagat atgaggtttc
     5581 cacctgagca tagatcgcgt aaaagattgc gaccccgaga acacgtcccc aggaatcaat
     5641 ggctgcgacc agcagagaaa gcgaaaccgt aattacggcc ccggcgatgg gaatgatgtt
     5701 gaagatgccc atgagaacgc caagcgcgta ggcataacgc acatgcagcg aaacatacac
     5761 aattgtgctg gccgttccca ggatcagcat gaggacgccc tgccccagaa gccactggcc
     5821 catgcgggcc tcggcacgga cgagggtctt gtcgaggcgc tggcgcatgt ccacggggaa
     5881 aaagctgagg agccagtggt atgctttttc gccttcgagc atgaagtaga cggtgaggac
     5941 gatgccggta atgacatcaa agagcttgct ggcccagtgc cgggctgaga acagcagata
     6001 tgtagcaaaa ttcgcggcaa agtcctgaag tctggcattg agcgaattaa tgtcaatccg
     6061 ctgcatcaaa ggcaggcggc ggacacgggc aaggagttga gggccgcgtg tgggtaactc
     6121 actcacaaac tctctcagat catgaatgac cggaggaaga gcaaagacaa agaacacggt
     6181 tgtgcatcct gccaccagca gaaggaggat cagaatggcc atgccgcggc cgggactcca
     6241 tttgccaatg cgcagtttca taatgccctg cacaatcggc ataaggacca cagcaaagag
     6301 cgcgctgacg taaatcagca ggagaatatt gcgaacatac caggcaagtg ccagtccaat
     6361 cacaagcgca aaggtaaaaa caatgtcact acgaagggtc cgactgctca tgcaggctgg
     6421 ccttccttaa aaagcagact cagagattcg acgatgtgcc gcactgtctc ttgtggagta
     6481 cgcgcagccg tctctaccac aagatgcgca ttgcggtagt ggggcagcct gctctcaaat
     6541 ctggtcttca ggcgttcgcg gtctttgagc acaggacgaa tgaccgcacc tggctgctgc
     6601 tcgcagcgcg caatcattgt ttccaatggg gcctcaagaa agacaacaca ggtgtcaggc
     6661 gcggagaaga tggcttcccg cgtggctgtt gtttctactg cgccaccgcc taccgcgagg
     6721 accagattgt gttcgcgcaa cagatcacga atggtctctg cttccatccg gcggaaagca
     6781 ggctcgccgc ggccggcaaa gatttcggcg atcgtggccc cggcacgctc ttcaagaact
     6841 gtatcggcat caagaaagcg ccaacccaac tgtggcgcaa gcagagcgcc gaccgtggat
     6901 ttacccgcgc ccataaagcc aatgagcacg atgcgggaga tacgggcggg aagttgtgcc
     6961 gttatcgcca tgtacaggaa ttataagtgc gctggcaaga ggaaggggcc aagtgaatct
     7021 tatcaggaaa gccgggtagg caatgcggcc ctggccgctc acagacccaa tagagtttgc
     7081 cagatacaat acaaaaagac tttctcatga gactgttccg caagaagctc aaacaggaac
     7141 acaaggtcat gttgcaggcg gaacgcgctg ggggagcgct tcttcctgaa taccaccggc
     7201 ctacgccgga gtgccccaat ccggagcggt ggcatatgta tgactccatg accgcagaag
     7261 ctgaggtgtt ggagtttctg caagtcctgg tgacgacaat caagcctggc ctcgtggtgg
     7321 agacaggggc atttctgggt gcaagcacca tttggattgc aaggggactc aaagccaatg
     7381 gctttggcaa agttgtcagt tgcgagttcg acccggtggt ctttacgaaa gcaaaagaga
     7441 agattgcggc atcggggctg gccgaatgga tcgagcttcg taatgaatcg agcctggaaa
     7501 tgcagataga tggaacgata gacctcttct tttccgacag tgacatgccc attcgctctg
     7561 cggaagtgaa acggtttctg ccacagattt ctcccacagg cgtggtgctt attcacgatg
     7621 ccagttcgca tttgaagatt gtccgggagg cggcattcga actggaaaga gaaggactgc
     7681 tcgcctgcgt ttttcttcca acgcctcgcg gcctggtcat tgcgcagaag cgcgaagggc
     7741 ggaagtgacg ctcagagttc catttcccag gtctgcccga tcaaatctac gccaaagctg
     7801 tgatgcggcc cttcgtggac aagccggaac cctgcctgct gataaatatg gtgcgctgcg
     7861 gcaagaatgc tttgcgtcca tagcgtcatc cttttgtaac cagcagtgcg tgcaaagctg
     7921 acacactcgt ttaccagcgc tttgccaagt ccgaggcccc gggcactcgg ttcaactaac
     7981 aagagacgca gcttcgcagt ttcgttctgc tccggatgtt tgaccagaaa gatgcagccg
     8041 aggcggtcac cattccgttc cgcgatccag cagcgctcac gcctggaatc gtagttgatg
     8101 atgaagtcgg cgacgatgcg cgctaccagc gcctcgaatt gctcgtccca tccatattcc
     8161 tgggcataaa gcgctccatg gcggctcaca acccagccca tatctccagg gcgatgctgg
     8221 cgcaggatgt agggcgcaga aggcctctcc atgctgagag cgttttccat ttcgcgcatg
     8281 gagcggacca gggcttcacg ttttgaaggc gggagttgat tgagaagatc acatgcctgc
     8341 ttgttggacc gctggtccag ttcgtcaaag gccgtccggc cctttgcggt gagagacagt
     8401 tgcatctggc gcgcatcagt ggtggatgcg gtctttttca atagccccat atcttcaaac
     8461 ttgcgaagga tacggctgag atatccggga tcgaggccaa gttcttctgc aatttctctg
     8521 gctgccatcc gcccatgtgc cagctcgtag agcacacggg cttcagtcag tgaatattgg
     8581 ctgttgagca gtccttcact caaagtcccg attgtgcgtg tgtagagacg gttgaagcgg
     8641 cgaaaggtgg cgatttcctc atccgattga agattttgag tagctgccat cagagttcac
     8701 ctcgtgtcaa ctaaattaat tgacttaggc aactttgtaa agcggtgtga cggaagaatt
     8761 taaaacgggc ctccgtctca aatatttgtg tccagaaatg tgtttctgaa cttgccttca
     8821 gggtcgtaat gcttcagcaa tcctttaaag tcgttcagct tctcgtattt tgcctgtagt
     8881 tggccgggcg gcatggtgaa gagtttgccc cagtggggac gcgggtcgaa cggttccaat
     8941 tgcttttcaa tcagtggcag gatttctttg actgcgggcc actccggttt ccaggtgaag
     9001 tgaatcgcga gcgagtcacg ttgataagcc atactcatcc agaggttgtc ggcggcgatg
     9061 gtgcgcagtt ctgtgataaa taaatgcggc gtgatgcggt cgcgtagttt ttcgaccgca
     9121 agaatggctt caaagctatg ctcgcgcggg acaaagtatt ccgattgcag ttccgcgccg
     9181 ctgctggggg taaagttcat gcggaagtgc ggcaggcgtt cataccacgg accgggagtg
     9241 cccatttgct ccgtgcaggg agtggcgtcg tgtccggcaa tgggatggag tttgtgtgtg
     9301 gcgagctttg ctccgtagaa atcggatggg agtgatgaag aaatgtccga atccatgcgc
     9361 tgcttgatcc agacctgcgt ggcgcgatgg tcctgccagt cagtgaaaag gctgacgctg
     9421 tagccgcttc cgaaaatggc atcgaggttg tcgcggagct gatcgaagga gaggttttca
     9481 tagacaagct ggcgcatctc gaagcggggc agcagatcga gagtgatttt cgtgataaca
     9541 ccgaggccgc cgagcgagac caccgcgccc tcaaaaacat catcatcttt cgcacgcgaa
     9601 agtgaggcaa tctggccatc tgctgttaca aattccagcg ctaaaacggc cgtggcaagg
     9661 ttgccgttgt gcatgccgga gccatgtgtt gcggtggcgc atgctcccgg cacggtgatg
     9721 tgtggcaggg aagcgagatt gtgcagcgcg tagccgtgac tgtcgaggta gggagcgagt
     9781 ttgccatatc tcacccctcc acccacggtg acggtgcgcg ccttttggtc aattgccatt
     9841 tcctccagat gcgagagcga aatctggttg tacttgctgt cagcgattgc gttaaaggag
     9901 tgtcgtgagc cgagcgctct gatcttctgg caacgtttga ccgtctgccg gacttcttcc
     9961 acagactttg gttcgtaaag gttatttgtg ctgtaggtgt aattgccggc ccaattcgtg
    10021 cgcgtgccag atatctgttc agcaggaaga tagtgagaca aggtagctcc actgacgagt
    10081 atgcctgaag tcttcaggaa atcgcgcttg ttcatggctg ggagagtttt atcacgaagg
    10141 gctacgattt tgccattaac ttatttgtta tctattcagg tgaatggagc aaatgatagg
    10201 attgcgaaaa tttcctgaaa ggcttgcaag taaatgaagc gtatttttgc cttagttctc
    10261 tgttttgtct ttgcgataag cgttctggct gccgataacg gatacaaaat cgtctatgac
    10321 ggcgggtctg ttacagcaat gaaagccgga aataaagctg aactttatat tgattcaaac
    10381 cagatccgct taaacgaaga tggaaaggac attgctgtta ttcccgcttc ggctgttaca
    10441 gaaatctctt acggtcagga cgtgcatcgc cgggtgggcg cggccattgg tttggctgtc
    10501 gtctcgtttg gtcttggagg actgatggcc cttacaaaat ccaaaaagca ctatgtgggg
    10561 cttacctggt caaacggaga tcaaaagggc ggatttgcca tgcagtgcga caagaatgag
    10621 taccgtggca tattggctgg tctggaaggc gtaaccggaa agaaggctgt taattcggac
    10681 acgatgacag tgaagaactg agcagcatgg atttcttttc aggcctataa ataaagcctc
    10741 tatgagaaaa atctacaccc tatcgcttcg aggggagatg ctggtgcatc ttcctcaaag
    10801 aacggcagga taaacccagt cccggcttct cattcagcgg gtcttctctt ccagttcttc
    10861 gtgcaggtgg cccatctttt cccgcttgac ctgcaaatag cgctggaagg tctcttccgg
    10921 ctcgacggta gcggagatgc gctccacgac gcgaattccg gcagattcaa gcgcttgt
//
LOCUS       DASRBV010000262         7453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_72334,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000262 DASRBV010000000
VERSION     DASRBV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72334"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..3489
                     /locus_tag="VFA02_09615"
     CDS             58..3489
                     /locus_tag="VFA02_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU10151.1"
                     /translation="MFSLSASAQFDNGSLVGTIRDSTGASIAGAAITVTNTATGIENK
                     ATTDGSGNWEIPSLRVGVYRVVASMPGFANAVADNVTLSVGSRQRIDLTLQPGQAQET
                     VEVTDVALQLETDSSQRGQVITQYQSAALPLVSRNYSDLLALVTGSRQAPTAAMTSSV
                     NSLTRAGSYNINGQRSMFNNFLLDGLDNNAYGESNQGFDNQIIAIPPDSVSQWQIVTN
                     NENAEYGRSSGATINVASQSGTNEYHFLLYEFIRNTDLNAAGYFKPTLTGTLGPVPFQ
                     KPTFNRNQFGMNFGGPVLHNRLFFFLDYEGFRQTLKPLGAFTLPTQNELNGILVVPVK
                     NPITGKIYSKGQQIPDSDFNPTSLQVVKFFRQIPLPVAGSPTTGIASNDYSRLVPFTD
                     NSDKGDLRLDWQQNASSSWFLRVSDRKETGVNYPAMPLPLDGQTNGTIRILDQQVALG
                     YTHLFGADKVLEARLGLSRTKAGKYTLSIGNNSITIPGLPSNPVVAGGLPSTSITNFS
                     GFGRQSTNPQWQNPALLDPKVNFTWVKGKHSMKFGYEYEHIWMAVNDNNPLYGSFGFT
                     SDYTNMGSGSPVADNYWADFLFGMPSSYALANYFVAHLRTTMDNVYAQDDWKITPKFT
                     LNYGLRWEYGSPYSEQHNYISNFDPVSQTVLTISPGAVAGNGITPVKGSGVYGSTLVN
                     PDLNDFGPRIGFAYSPDGKTVIHGGFGVSYVHYTRAGSGDILGINAPQAQFASVGSQA
                     VKPSSATHCPAGAPATNCFVTMDQGFPSSLTVFNAATDNITWIPKDTRDSYVESYFLS
                     VQRQLAKNILFDVAYVGNHGVKLQGFLNANQKIPSTNFTTRPFSNWPSDITEALNAFY
                     SHFDSLQTRYEQRFVGGLTLLNSFTWEHSLDNASASLEGNTPSPQDGTHLSADYSQSD
                     YNLPIANITSLVYELPFGHGRPFLSNANGFVDAILGGWQISGINTMQAGTPFNITYSP
                     SSSNQVSQQITASYRGANLYRPNRVPGQPLIKKSKLPGTGYIQYVNYAAFVLPPTKDS
                     AGNLLSPFGNSSRNPGRTPAFYQTDFAANKIFNLPIESMKLEFRSEFYNIFNHTNYYL
                     PSSGLGGTLGSVAGVYAPGKTVPVSAIQGGTPSTGGQVTSTFQPRIIQFGVKLTY"
     gene            3577..5412
                     /locus_tag="VFA02_09620"
     CDS             3577..5412
                     /locus_tag="VFA02_09620"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZU10152.1"
                     /translation="MAEELDELRHQVQELTARVWRLEQAIGQRQAERAVPEAVSADPV
                     AEAQTATAPEREGPLTVSGPERSDKHTDGSLESRIGSQIFNRIGILAVLIGMAWFLKF
                     AIDNHWIGPVGRIVVGLIAGVGMIVWSERFRRKGYPAFSYSLKAIGSGILYLALWAAF
                     ELYQLVPSSVAFAGMALVTAWNGFMCWVQDAELLALYAIVGGFGTPLLLSTGENHELA
                     LFSYLLLLDIVVLVLVALRPWSRLLLGAFAGTVFYSVAWYVRFYDAYQFGLTAFFITA
                     FFLLFAVAPKLLRGLQIADTSRLSVQDNFVLVLLPLANAALGFLEFYTLLDQPGRKWA
                     QPWTAILFAAFYLLLLKIPSRHGAEAKPVILSSLHLAIAVVFLTIAVPLEAHGRWITI
                     GWLTEGVVLLWVARRLELFLLRILSPGALALGFFALIVLNPAASATVILNARFATYLV
                     GIAAFGCAAWIATKAASEEQTGAFPWAGIAGGSVIAVNALIMIAVCLEIHSYWWNQVS
                     GGQGWGGFHEHRMYAQFTYSAWTMLFGAVLLAAGFWKSSAFLRWQALILIAGSIGKVF
                     LVDTSELSQGYRILSFLGLGVLLLAVSFAYQRDWLNLRRQRGARE"
     gene            5409..6644
                     /locus_tag="VFA02_09625"
     CDS             5409..6644
                     /locus_tag="VFA02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HZU10153.1"
                     /translation="MSARLAALLLLLFVQPEQRYFRYVRPVAGIAAGQKQTCVALDVS
                     VFAHAAPGLADLRLYHDGQETPYALRSSAPVPAEHTELQPVNLGTRQGSVVFDAEMPQ
                     GSYSALRLEIARKDFIATVDVAGSQTKNASDATQLGQYTIFDLTGQKLGRSMVLHLPV
                     SDFHYLHWRISSPVKPDDVTGLFLERELLRDAEYVTVAEQKSMAQQGRSTVIEFSVPP
                     HVPVDRVAFLPADGAANFSRYVSVEVTPEDAKQHAPTSYSGEIRRVHILRGSRRIDDE
                     RLVIDVDSTGMTAASRWTVKVDNGDDAPLALNAVRLEMQKQEICFEAIPGTNYKLYYG
                     DAALSSPEYDYARLFQPDKDAARGMLGPEEENPQYQTRPDTRPFTERHPALLWIALVA
                     AVAILGSIALQTARAGRVK"
     gene            6728..>7453
                     /locus_tag="VFA02_09630"
     CDS             6728..>7453
                     /locus_tag="VFA02_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZU10154.1"
                     /translation="MKVALAQINPTIGDFTGNIAKILDYTRQAAARGADLVLFPELAV
                     CGYPPADFLDKPSFLARSEEAVQEIAAVTAELNIASIVGYVTHAPAGTGKRVMNSAAL
                     LRKGEVDFVQSKMLLPFYDVFDDQRYFAPAEKQHLVYFCGKRLALTICEDAWNDKNFW
                     HKRLHAVDPVEELMREGGNLILNISASPYWRGKRKTRREMLAAIARHHCAPVVMVNQV
                     GGMDSLIFDGSSLVLSRDGEVIAQ"
BASE COUNT         1590 a         1854 c         2175 g         1834 t
ORIGIN      
        1 atgcaatcac atcattctgg tcagttccgc ctgcttctgg caggcattgt tctgtttttg
       61 ttctctttgt ctgccagtgc gcagttcgat aatggaagcc ttgtgggcac cattcgagac
      121 tcaacaggcg cgagtattgc tggtgcggcg atcactgtaa ccaacacggc tacaggaatc
      181 gaaaacaagg cgacaacaga tggcagcggg aattgggaga ttccgtcgct gcgcgtgggc
      241 gtgtatcgtg ttgttgcttc gatgccggga tttgcaaacg cggtggcgga caacgtaacg
      301 ctgtctgttg gcagccgtca gcgcattgac cttacattgc agcccgggca ggcgcaggag
      361 acagtggaag tgaccgatgt tgcgctccag cttgaaacgg attcgagcca gcgcggacaa
      421 gtcatcacgc aatatcagag cgcagcgctt ccgctggtga gccgtaatta ttctgatctg
      481 ctggcgctgg ttacagggtc gcgtcaggcg ccgacggctg cgatgaccag ctcagtgaac
      541 agccttacgc gggccggttc ttataacatc aatggccaac gcagcatgtt taataacttt
      601 ctgctggatg ggttggacaa taacgcttac ggggaaagca atcagggatt cgataatcaa
      661 atcattgcca ttccgccgga ctcagtttcg caatggcaga ttgttacgaa caatgaaaat
      721 gctgaatatg ggcgttcatc cggggcgacg atcaatgttg cttcgcagtc cgggacaaat
      781 gagtaccact ttctgcttta tgagttcatc cgcaataccg atttgaatgc agcgggttat
      841 ttcaagccaa cacttacggg cacactcggc ccagttcctt ttcaaaagcc gacatttaat
      901 cgcaaccagt ttggaatgaa ttttggcggg cctgttctgc acaaccggct tttcttcttc
      961 cttgactacg aaggatttcg ccagacactg aaaccactgg gtgcatttac attgcctacg
     1021 cagaacgaac tgaacggtat tctggtggtc ccggtgaaga acccgattac gggaaaaatt
     1081 tatagcaagg gccagcagat tccggacagc gactttaatc ctacttcgct gcaggtcgtt
     1141 aaattcttcc gccagattcc tcttcctgtt gccggatcgc cgacgaccgg tattgcttcc
     1201 aacgattatt cgcggctggt gccatttaca gataattctg acaaaggcga tttgcgtctg
     1261 gattggcaac agaatgcttc aagctcatgg tttctgcgcg tcagtgaccg taaggaaaca
     1321 ggcgtgaatt atcctgcgat gccgttgcca ctggatggcc agacgaacgg gacgatccgg
     1381 atcctggacc agcaggttgc gcttggatac acgcatctgt ttggtgcgga caaggtactg
     1441 gaggcgcggc tggggctttc gcgcacgaag gcagggaagt acacgctttc gattggcaac
     1501 aattcgatca cgatacccgg gctgccctcc aatccagtgg tagctggtgg attgccttca
     1561 accagtatta cgaatttcag cggatttggg cggcaaagca caaacccgca atggcagaac
     1621 ccggcgttac tcgatcctaa agtcaacttc acctgggtga agggcaagca ctcaatgaag
     1681 tttggatatg agtatgagca catctggatg gcggtgaatg acaataatcc actgtacggt
     1741 tcgtttggat ttacttcgga ctacaccaac atgggttcgg gatctcctgt agctgacaat
     1801 tattgggcgg attttctgtt cggaatgccg tcttcgtatg cgctggccaa ttattttgtg
     1861 gcgcacctgc ggaccacgat ggacaacgtc tacgcgcagg acgactggaa gatcacgccc
     1921 aaatttactt tgaattatgg tctgcgctgg gaatatggtt ctccatactc cgaacagcac
     1981 aactacatct caaacttcga tcctgtttcg cagactgtgc tcacgatcag tccgggcgcg
     2041 gttgcgggca atggaatcac gcctgtgaag gggagcggag tttatggcag cacgctggta
     2101 aatcctgacc tgaatgattt tggtccgcgg attggttttg cctactcgcc ggatggaaag
     2161 acggtcattc atggcggatt tggcgtcagc tatgtccact atacgcgcgc cggttccgga
     2221 gacattctgg gcatcaatgc tccgcaggcg cagtttgcca gcgtgggatc gcaggctgtg
     2281 aaaccatcga gcgcgacaca ttgcccagca ggagcgccgg ccaccaactg ctttgtcacc
     2341 atggaccagg gttttccttc atcgcttaca gtctttaatg cggcgacgga caatatcaca
     2401 tggatcccta aagacacgcg ggacagctat gtggagagct actttttgag cgtacagcgc
     2461 cagcttgcaa agaacattct ttttgatgtg gcgtatgtgg gcaaccatgg cgtaaagctc
     2521 caggggttcc tgaatgcgaa ccagaagatt ccatctacga actttacgac gcggcctttc
     2581 agcaactggc cttctgatat tactgaagcg ctcaatgcct tctactcgca ttttgactcg
     2641 ctgcaaacac gatacgaaca gcgctttgtt ggaggattga cgttgctaaa ttcgttcacg
     2701 tgggagcatt cgctggacaa cgccagcgcg tcgcttgaag gcaatacgcc gtcgccgcag
     2761 gacggaacgc atctgagcgc ggattattcg caatcagatt acaacctgcc catcgcgaac
     2821 attaccagcc tggtgtatga gctgcccttt gggcatggac gcccgttcct ttccaatgca
     2881 aacggatttg tggatgcaat cctgggcggc tggcagatca gcgggatcaa tacgatgcag
     2941 gccggaacac ctttcaacat tacgtattct ccaagctcgt cgaaccaggt ttcacagcag
     3001 attacggcga gttaccgtgg agcgaacctg taccgtccaa accgggtgcc ggggcagccg
     3061 ctgattaaga aatcgaagct gccggggacg ggatatatcc agtacgtgaa ctatgcggcg
     3121 tttgttctgc ctcccacgaa ggacagtgct ggaaacctgc tgagtccttt cggcaattca
     3181 tcgagaaatc cggggaggac tccggcgttt taccagactg attttgcagc gaacaagata
     3241 ttcaacctgc ctatcgaaag catgaaactt gagttccgct cagagtttta caacatcttt
     3301 aaccatacga actattacct gcccagcagc ggtctgggag ggacgcttgg cagcgtagca
     3361 ggcgtctatg cgccgggcaa gaccgttcca gtcagcgcaa tccagggagg tactccaagc
     3421 acaggcggtc aggtgaccag cacctttcag ccgcgcatta ttcagtttgg agtgaagctg
     3481 acatattagg cctgttgacc ctatcagagc cgacagagaa acagaggcca cattactggt
     3541 ctctgttttt tcttttcatg ctgtacatta aacgggatgg ctgaagagct ggatgaactg
     3601 agacatcagg tgcaggaact gacggcccgc gtatggcggc tggaacaggc aataggacag
     3661 cgccaggcgg aaagagctgt tcctgaagct gtttctgccg atcctgttgc ggaagcgcag
     3721 actgcgacgg cacctgaacg agaagggcct ttgactgtgt ctgggcctga acgcagcgac
     3781 aagcatacag atggctcgct tgaaagccgc attggttcgc agattttcaa ccgtatcgga
     3841 attcttgctg tgttgatagg catggcatgg tttctgaagt ttgcgattga taatcactgg
     3901 atcgggccgg ttgggcgcat tgtggttggc ctgattgcag gcgttgggat gattgtctgg
     3961 tcggaacgat ttcggaggaa gggatatccc gccttttctt actcgctgaa ggccatcggc
     4021 agtggcattt tgtatctggc gctgtgggct gcatttgagt tgtaccagct tgttccatcc
     4081 agtgttgcat tcgcgggcat ggccctggtt acggcatgga acggattcat gtgctgggtg
     4141 caggatgcgg agctgctggc gctctacgcg attgtgggcg gctttggtac tccgctgttg
     4201 ctttcgaccg gagaaaacca cgagcttgca ctctttagtt atttactgct gctggacata
     4261 gttgtgctgg tattggtggc cctgcgtcca tggtcgcggc tgctgctggg cgcatttgcg
     4321 ggcacagtct tttatagtgt ggcctggtat gtccgctttt atgatgcata ccagtttggg
     4381 cttaccgcct tctttattac tgcattcttt ctgctctttg ctgttgctcc gaaactactt
     4441 cgtggattac agattgcgga cacttcgcgc ctctccgttc aggacaattt cgtccttgtc
     4501 ctgcttccac tggcaaatgc agcgctgggc tttcttgagt tttatactct gctggaccag
     4561 cctgggcgca agtgggcaca gccgtggaca gcgattctat ttgccgcgtt ttatctgctg
     4621 cttctgaaga ttccgtccag acatggcgcg gaggcgaagc cggtcattct ttcgtcactg
     4681 catctggcga ttgcagttgt ctttctcacc atagcggttc ctcttgaagc gcatggccgc
     4741 tggattacga tcggatggct gactgaggga gtggtgttgt tatgggtggc gcgacggctg
     4801 gagctctttc ttctgcgaat tctttcgccc ggggcactgg cacttgggtt tttcgcgctg
     4861 attgtgttga atccggcggc gtctgcaacg gtcatcctga atgcgcggtt tgctacgtat
     4921 cttgtgggca ttgctgcatt cggctgcgcg gcgtggattg cgacgaaagc tgcttcagaa
     4981 gagcagactg gggcttttcc atgggcaggc atagcaggcg gttctgtcat tgcagtgaat
     5041 gcgctgatta tgattgcagt ctgcctggag atccattcct actggtggaa ccaggtttcg
     5101 ggtggacagg gatggggcgg cttccacgaa cacaggatgt atgcgcaatt tacgtactct
     5161 gcctggacga tgctgttcgg ggccgtgctg cttgcagcgg ggttctggaa gagttcggca
     5221 ttcctgcgat ggcaggcgct gattctgatt gccggaagca ttgggaaggt ctttcttgta
     5281 gacacaagcg agttaagtca ggggtatcgt attctcagct ttttgggact cggggtgctg
     5341 ctgcttgccg tcagctttgc ctatcagcgt gactggctca atctgcgcag gcaacggggg
     5401 gcccgcgaat gagcgcccgt ctagctgcgt tgctattgct gctgtttgtg cagccggagc
     5461 agcgctactt ccggtatgtt cgccctgtag ctggtatcgc cgcggggcaa aaacaaacat
     5521 gcgttgctct ggacgtatct gttttcgcgc atgcagcgcc ggggctggcg gacctgcggc
     5581 tctatcacga tgggcaggag actccatatg ccctgcgctc ttctgcgcct gtaccggcag
     5641 aacatactga attacagccg gtcaatcttg gaacacggca aggcagcgta gtttttgatg
     5701 ctgagatgcc gcagggcagt tacagcgctc tccggcttga gattgcgcgc aaagacttca
     5761 ttgcaacggt ggatgtggcg gggagccaga caaagaatgc ttctgacgcg acgcagcttg
     5821 gccaatacac catctttgac ctgactgggc agaaactggg gcgcagcatg gtgctgcatc
     5881 tgcctgtttc tgactttcat tacctgcatt ggagaatcag cagcccagtc aaaccagatg
     5941 acgtgacagg acttttcctt gagcgggagt tattgcgcga tgccgaatat gtgacggtcg
     6001 cggaacaaaa aagtatggcg cagcaggggc gcagtactgt tattgagttt tctgttccgc
     6061 cgcatgttcc ggtagaccgt gttgcattct tgcctgctga tggagcagct aatttcagcc
     6121 gttatgtgag tgttgaagtt acgccggaag acgcaaagca gcacgcaccg acaagctatt
     6181 caggtgaaat ccggcgcgta cacattctgc gcggaagcag acgaattgat gatgagcggc
     6241 tcgttataga cgtggacagc acgggaatga ccgcggcttc cagatggaca gtgaaggtag
     6301 acaatggaga cgacgctcct ctggcactga atgcggtccg gcttgagatg cagaaacagg
     6361 agatctgttt tgaggcgatc ccaggcacaa attacaagct ttactatggc gatgctgctt
     6421 taagttctcc tgagtatgac tatgcgcggc tttttcagcc tgacaaagat gcagcacggg
     6481 gaatgctcgg gccggaagag gaaaatccgc agtatcagac gcgcccggac acgcggcctt
     6541 ttacagaacg gcatcctgcg ctcctgtgga ttgcgcttgt agcggccgta gcaatcctgg
     6601 gaagtattgc gctgcagact gcaagggcag gccgggtgaa ataagtcgaa tggccgtccc
     6661 actccttttg gcgaccccga ctggctaaat gagctggccg aaggccccgc gttgttacac
     6721 taaagctgtg aaggttgcgc tcgcccagat caatcctacg attggtgatt tcaccggaaa
     6781 cattgccaaa attctcgact atacacggca ggccgcggcg agaggggccg atcttgtgct
     6841 ttttccggaa ctggcggttt gcggatatcc tccagcagat tttctggata agccttcgtt
     6901 tctggcccgg tcggaagagg cggtgcagga gattgctgcc gtgacggctg aactgaatat
     6961 cgcaagcatt gttggatatg tgacgcatgc tcccgcgggg acggggaagc gggtgatgaa
     7021 ttctgcggcg ctgctgcgca agggcgaggt ggactttgtc cagtcaaaga tgctgctgcc
     7081 gttttacgac gtgtttgatg accagcggta ttttgcaccg gcggagaagc agcatctggt
     7141 gtacttctgc gggaagcgtt tggcgctgac catctgcgaa gacgcatgga acgacaaaaa
     7201 cttctggcac aagcgtcttc atgcggtaga cccggtggaa gagttgatgc gtgagggtgg
     7261 caatcttatt ctgaacattt cggcatcgcc ttactggcgg ggaaaacgca agacgcgtcg
     7321 cgagatgctg gcggcgattg cgcgccacca ctgcgctccg gtggtgatgg taaaccaggt
     7381 gggcggcatg gacagcctca tctttgacgg ctcaagcctg gtgctttcgc gggacggtga
     7441 agtgattgcg cag
//
LOCUS       DASRBV010000263         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_683730, whole genome shotgun sequence.
ACCESSION   DASRBV010000263 DASRBV010000000
VERSION     DASRBV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683730"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2595)
                     /locus_tag="VFA02_09635"
     CDS             complement(<1..2595)
                     /locus_tag="VFA02_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HZU10155.1"
                     /translation="MKIGIGRGMALSAMFVSSVLWAQDVRQVVKSLPSNSQAVIERLG
                     ELDHLDAGEWKFHAGDVPHGESVSLDDSGWQTVKPGFRTSGESAWFRRWVEVPKTLHG
                     YDLTDTRIWFRFEAYANGPMPQIIYFNGSRVALGDDLEPIVLLDHAKPGQKVLIAVKL
                     LQTVDQKTFHNADFKIDFSENRPNPDDLRKEFLSAALLVPSLSTNQQGDEATLEKAVH
                     AVDLKALDAADQTKFDASLKEATSDLEALKPLLKGATFHLTGNSHIDAAWLWPWTETV
                     DVVKRTFSTALQLMNEYPNYTYTQSAAAYNYWMATKYPDINDEIKKRIKEGRWEIVGG
                     MWVEPDLNMPSGEATARSILLGKRFYKQEYGVDVRIGWNPDSFGYNWQLPQIYKKSGI
                     DYFVTQKMAWNDTNQLPFKLFWWESPDGSKVLTYFPHDYANNNLNPVRLSADLTTARK
                     WSPGMTEMMDLYGIGDHGGGPTRAILDEGEHWAQPDKIVPNYQFGTAQSYFSTVEKKI
                     APESPEWDYFKIMKGYTPPPTPPAGQIAIPTWKTEMYFEYHRGVMTTQAQHKWNMRHS
                     EMWALNAEKYASLAWLDGKSYPSDKLTDAWRTITFNDFHDLAAGSGIGIIYKEAQEQF
                     DKTRLETDKISQDSLKTIAARINTKAAGEVPVLVFNPLAWERSGWFEVTVQMPNATSG
                     VSMLDAKNQVLPSLVLSNDKATNTYKLLVLAKNVPSMGYTVLHAVPGEKPFTSDLKVS
                     GLTLENSLLRVTVDKTTGCITSIYDKKTNFESLAPGACGNELQAFKDTPKDYDAWNVD
                     PGTLDVPPTRLTQADSVELVEKGPARASIRVTRHWQNSKFVQEIQLAAYSDEVNIVND
                     IDWHETHIL"
     gene            2857..>3636
                     /locus_tag="VFA02_09640"
     CDS             2857..>3636
                     /locus_tag="VFA02_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10156.1"
                     /translation="MKLHFLPAFLAFACSMSFAQGTKLWTQQRFDEFEKGTPKGVSIR
                     SNGLLESGPESRQIAITPSTYIWSVAADTAGNAYAGTGSPASVLRISTDGKIAKLFSS
                     KDLSVQAVAVAKDGSIYAGTLPSGKVYKLPAGATDLDETKATVVFDPAQTEAKPKYIW
                     ALAFDSEGRLYIATGAPAAIYRLNPAAASTKPELFFKSDEQHIRSLAFDKSGKLIAGS
                     DGSGLIYRIDGNGKGYVLYDAPKHEITSLAVADNGTIYASAV"
BASE COUNT          817 a         1055 c          894 g          870 t
ORIGIN      
        1 caaaatatgg gtctcatgcc agtcaatatc attcacaata ttgacttcat cggagtacgc
       61 cgcaagctgg atttcctgca caaacttcga gttctgccag tgacgcgtca cgcgaatcga
      121 agcccgcgcc ggcccttttt ctaccagctc gaccgaatcg gcctgcgtca ggcgtgtcgg
      181 cggaacatca agcgtgcccg gatcaacgtt ccaggcatcg tagtccttcg gcgtgtcctt
      241 gaacgcctgc aactcattac cacacgcgcc aggagctagc gactcgaaat tggtcttttt
      301 gtcgtaaatg ctcgtgatgc agcccgttgt tttgtccact gtaacgcgca gcagcgagtt
      361 ttcaagagtc agaccgctca ccttcagatc gctggtgaaa ggcttttcgc ccggaacggc
      421 atggagcaca gtgtagccca tggagggcac gtttttcgcc agaacgagca gcttataggt
      481 gttcgtcgcc ttatcatttg aaagcaccag cgacggcaac acctggtttt ttgcatccag
      541 cattgaaacg ccggaggtgg cattcggcat ctgcactgta acctcaaacc atcctgaacg
      601 ttcccaagcc agcgggttaa agaccagaac cgggacttcg ccagctgctt ttgtattgat
      661 tcgagcagca atcgtcttca gcgagtcctg cgaaattttg tccgtttcaa gacgcgtctt
      721 gtcgaactgc tcctgcgcct ctttgtagat aatgccgata ccagaaccag ccgcgaggtc
      781 gtggaagtca ttgaaggtga ttgtcctcca cgcatccgtc agcttatcgc tgggatagct
      841 ttttccatcc agccatgcca gcgaagcata tttttcagca ttgagtgccc acatttcact
      901 gtggcgcata ttccacttat gctgagcctg cgttgtcatt acgccacggt gatactcaaa
      961 gtacatttcc gtcttccagg tcggaattgc aatctggccc gccggtggcg ttgggggcgg
     1021 cgtgtagccc ttcatgattt tgaagtagtc ccactccggt gattccggag caatcttctt
     1081 ctcgaccgtg ctgaagtagc tttgcgctgt tccgaactga taattgggga caatcttgtc
     1141 cggctgcgcc cagtgctcgc cctcgtccag aattgctcga gttggaccgc cgccatggtc
     1201 gccaatcccg tagaggtcca tcatctccgt catgcccgga gaccactttc gcgcagtcgt
     1261 cagatctgct gaaagacgta caggattgag attgttgttc gcataatcat gtggaaaata
     1321 cgtcagcacc ttgctgccat ccggcgactc ccaccagaag agcttgaacg gaagctgatt
     1381 cgtatcgttc catgccatct tctgtgtaac aaagtaatca atgccggact tcttataaat
     1441 ttgcggtaac tgccagttat aaccaaatga atccgggttc catccaatgc gaacatctac
     1501 gccatactcc tgcttataga agcgcttgcc cagcaggatg gagcgagccg tcgcctcgcc
     1561 ggatggcatg ttcaaatcag gctcaaccca catgccgccc acaatctccc agcggccttc
     1621 cttaatgcgt tttttaatct cgtcattgat gtccggatac ttcgtagcca tccagtagtt
     1681 gtaagcagca gctgactgcg tataagtgta gttcggatac tcattcatca actgcaacgc
     1741 agtcgagaag gtacgcttta ctacatccac tgtttccgtc cagggccaca gccaagcggc
     1801 gtcaatgtgc gagttcccag tcagatgaaa agtcgccccc ttgagcagcg gcttaagcgc
     1861 ttccaaatca ctcgtcgctt ctttcaaaga ggcatcgaac ttcgtctggt cagccgcatc
     1921 cagcgccttt aagtccactg catggacggc cttttccagg gttgcctcat cgccttgttg
     1981 attggtcgaa agactcggaa cgagtagcgc tgcggaaagg aactccttcc gcaagtcgtc
     2041 cgggttgggc cggttctcag aaaaatcaat cttgaagtcg gcattgtgaa aggtcttttg
     2101 gtctacggtc tgcaacagct tgaccgcaat caggactttc tgccctggtt ttgcatggtc
     2161 gagcagcaca atcggctcaa ggtcatcgcc tagggcgacg cggctgccgt taaaataaat
     2221 aatctgcggc atggggccat tcgcgtacgc ctcaaagcgg aaccagatac gcgtgtccgt
     2281 cagatcatag ccgtgcagcg tctttggtac ttcgacccag cgccgaaacc atgctgattc
     2341 tcccgatgtc cggaatcctg gctttaccgt ctgccagcct gaatcatcca gactcacact
     2401 ctcgccatgc ggaacgtcgc ctgcatgaaa cttccattcg cccgcgtcca gatggtccag
     2461 ttcaccaagc cgttcgatca cagcctgcga attcgaaggc agcgatttca cgacctgacg
     2521 aacgtcctgt gcccaaagca cggaggaaac aaacatggca gacaatgcca tgccccgccc
     2581 tattccaatc ttcacctgca aactcctcgc tcctgattcc gcgcagtgtc ccacctcaga
     2641 aagcactagg gaaaactgcc agtagagacg gttggattat aaccttttaa agccataagt
     2701 ggaaacgttc ccattatttt gcttccgaat gctgtcagga taagggcgca gaatcttatt
     2761 aagggcttac ctgtcctgaa gcgcactgaa catgttcaca cctgattggt gagcgccctg
     2821 tacgctgtct tccgtaccta gtaccctgtt agaagaatga agctccattt cctgcctgca
     2881 tttctggcct tcgcctgctc tatgtctttc gcccagggaa caaagctctg gacgcagcag
     2941 cgcttcgatg agtttgaaaa aggcacgccc aaaggtgtgt ccattcgcag caatggcctc
     3001 cttgaatcag gcccggaaag ccgacagatc gcgatcactc cgtctaccta tatctggtct
     3061 gttgccgcag ataccgcagg aaatgcctat gcaggcaccg gatcgcctgc ctcggtcctg
     3121 cgcatctcga ctgacggaaa gatcgcgaag ctgttttcga gcaaagatct ttctgtgcag
     3181 gccgtcgctg ttgctaaaga tggctctatt tacgctggaa cgctgccctc cggcaaagtg
     3241 tataagctgc ctgcaggggc aacagacctc gacgaaacca aagccactgt cgtcttcgac
     3301 ccggcgcaaa cagaggccaa acccaagtac atctgggcgc ttgcattcga cagtgagggc
     3361 cgcctttata tcgccactgg agcaccggcc gcaatctatc gcctgaatcc cgcagcggca
     3421 agcacaaagc ccgagctttt cttcaagagc gatgagcagc acatccgctc tctggctttt
     3481 gataagtctg gaaagctcat cgcgggttcg gacggaagcg gtcttatcta ccgcatagat
     3541 ggcaatggca agggctacgt cctttacgac gcgccgaagc acgaaattac gtctctcgcc
     3601 gttgccgata acggcaccat ctacgcctcg gccgtc
//
LOCUS       DASRBV010000264        17157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_422124, whole genome shotgun sequence.
ACCESSION   DASRBV010000264 DASRBV010000000
VERSION     DASRBV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 17157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17157
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422124"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..3916)
                     /locus_tag="VFA02_09645"
     CDS             complement(299..3916)
                     /locus_tag="VFA02_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10157.1"
                     /translation="MQMLKEAKENFRAKLITGIRYGSLALAVTFAAAAGSFFFSDHAN
                     AQAISVNGGSIQGVITDSSGAAVGNATITVSSQQTGFTKTLKTDAAGLYTVGPLTPGS
                     YQVIVEASGFQKLVASTVVLTGTATNGNYKLAVGAATEEVTVNAGAVQVNTEQAGVQN
                     VITAQQIETLPINGRNFLDLAQLEPGVMLQSGQDFDPTKAGYSAIGFSGVSGRTTRIL
                     LDGQDITDETVGTTIFNVSQGAIDQFQISRSNNDVSGEIGSTGQVLVSTRTGSNAFHG
                     EAFYDFQDHSTGFAKVNGQDAPFQRNQFGGSIGGPVLKDRLFFFANAERIKQDESSPA
                     QIGPVFADILAKYPNVPAPYRQTYSTGRLDYSGPWGVHYFARINYDVNSTITSGNNSY
                     DNYANRDNTWGIAGGADFVKGNFTHSFRGSYEKFHNLIVDATGSGVYLAMPGLLIRDA
                     SQGLYTGPNDLAPQDTYQSDKQLRYDGSWTKGAHNIRYGASLNRILQGGFASFFGLAP
                     RVSLSPAALIKDSNGNPIAPGDPLNGYNASVGVRFGNGLGYFTPEPTFGQPAGGSDDW
                     RTGAYVSDSWKVKPQLTVNYGIRWLRDTGRTDNWIDPIPCSTINTSIFPNPPCTGNQL
                     ILDQFGVGLGKRIRQPNFDFAPQVGFTYSLDSAGKTVVRSAVGIFRENIVFNAVQFDT
                     PFKLQTGLFNDYSHTVCGGTYVFKFPDGSTTDNIDGDPLTSVCREPLAVSGPKFVKLQ
                     AEYQQISKAGGAAQNGSFIGNTLAIPGGDAAYSPNFKTPYSIHFNFGIQRELWSGAVL
                     SADYVHQATLHIAQSIDVNHIGDAKYLNKTAAQNAINATLTQFGVTNIDDAIANGATI
                     SDFANNGLDSGNVYLSGAPAAQYGLTPDEGAAFPGANPNMGNGFFNFPAGRSGYDAFQ
                     LNLRQQARHPLKGLADSNFEFSYAYSKFVSSSTGGSDQFFSGAAYDYNNPTQYIGFGG
                     NDHRHNISFGGAATIIHGPQIAVIGHFRSAAPSNLTLDTLAGSTAQIFMTDVTGDGTI
                     GDLAPGTNPGQFMRGTSGSDLKKFIVNYNNKYAGQLTPAGRALVNNGLFNPTQLSELG
                     AVQQPIYNGFSSVFQNPMFKSLDASVSYPFHLKFISESVTLVPKVSMYNVANFANWGG
                     TTTTLVNQSNAGSDGTGAEGYVNGDNGFSFKNQTRVGRGSGTFDQGGPRSTEFQLTFN
                     F"
     gene            4142..5005
                     /gene="htpX"
                     /locus_tag="VFA02_09650"
     CDS             4142..5005
                     /gene="htpX"
                     /locus_tag="VFA02_09650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZU10158.1"
                     /translation="MNTFKTALLMTALTLFLLFLGEHFGGRDGLVLAFIFAAGMNFFS
                     YFFSDKLALAMYRAQPITREELPRVYQVVERLTQRAGLPMPKIYVIPTDSPNAFATGR
                     NPKHASVAVTQGILNLLNDDELEGVLAHELGHVRNRDILTSSIAATLAGAITMLARMG
                     YWASLFGGYGDSRDRERGGGGLGALFMLILAPIAATLIQLAISRSREYEADATGAHIT
                     GNPYALASALEKLDAYSKRLPMQASPSNAHLFIVQPLVSARDFANLFSTHPPIPKRIE
                     RLIGRSSMYGS"
     gene            5066..5554
                     /locus_tag="VFA02_09655"
     CDS             5066..5554
                     /locus_tag="VFA02_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HZU10159.1"
                     /translation="MKTLLRSLILLLIVLWLGGVMFFPIVAATAFSSLPDTHAAGTIV
                     GHCLRVLHYEGIFAGALIIVLFVIAYRTGAYLRHVIAPVFVTLIMLALTAFSQFWIIP
                     KMEGYRLATGGAVDAVAPGDPNRVAFNKLHVTSERVEEGVLLAGIVLVVLVAWESTPK
                     KP"
     gene            complement(5559..6305)
                     /gene="rph"
                     /locus_tag="VFA02_09660"
     CDS             complement(5559..6305)
                     /gene="rph"
                     /locus_tag="VFA02_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZU10160.1"
                     /translation="MQTTPVSFFRQGGRAADALRPVRLTPHYVAVAEGSVLIELGNTR
                     VLCNASVEQSVPAWLRNSGRGWVTAEYGMLPRATLTRTPREAERGKIGGRTHEIQRLI
                     GRSLRAVVDMQALGERTVVLDCDVLQADGGTRTAAITGACAAMAIAFDRLVAAGTLPK
                     SPMRQLVAATSIGIVENTILLDLAYEEDSQAEVDMNVVMTEDGGLVEVQATAERKMFA
                     RPQMDQMLDTAALGIQQLLAEVRSMLQKAV"
     gene            6339..7139
                     /locus_tag="VFA02_09665"
     CDS             6339..7139
                     /locus_tag="VFA02_09665"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZU10161.1"
                     /translation="MTSLVVMDDTFDLAEMDRALAGTPFAGKLHFFPSIHSTNTYAMQ
                     QAEAGAPEGSVYFADEQTAGRGRGAHQWASPPGSGLYVSVLLRPQIPPAEILWLSLAA
                     GLAVREAVRQVTALECDLRWPNDLLFGRRKFCGILTELNAEVTRIRHVVVGIGINVHQ
                     EKFPPGLHDIATSLRMETGREWPRQQLLIALLQSLHREVSGLSREAASGILQRLEAAS
                     SWVRGKRVRVEEGPGFSGVTEGLDARGFLQVRTAEGLRTVYSGGVREA"
     gene            7155..7979
                     /locus_tag="VFA02_09670"
     CDS             7155..7979
                     /locus_tag="VFA02_09670"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZU10162.1"
                     /translation="MLLVLNVNNTNTVIALYTLRQDGLADKLRATWRISTRHVQTADE
                     YGILVRSLLQSEGVEGPAIKSVVIASVVPPVDWVLRQFCERYFHQKPLFIEPGVKTGL
                     PILTDNPSEVGADRVANCVGAFAKYGGPAIVVDFGTATNFDVVSRKGEFLGGAIAPGL
                     NVSAEALFARAARLPRVEIKRPAKIIGTNTVDNLQIGLFYGHIGLVDYILERMVAKLG
                     EGTRCIATGGLAHLIASESKFITEIDDMLTLDGLRIIYDRNRSAKAHPHAGEMQQP"
     gene            8125..8808
                     /locus_tag="VFA02_09675"
     CDS             8125..8808
                     /locus_tag="VFA02_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10163.1"
                     /translation="MENYFNYFTEIEEHFQRRRGSLLLLSTLDWALIETWREAGVPLE
                     AVLRGIDATFEKYEARKTRTRVRRINGLAYCAQEVMSAVEEMKEAAAGAASALNQAAP
                     PPGFEPERIALHLESCAEALRNVQINETAQRLLALASGVRMQQPDLEDLERTLTTLEE
                     KLFAHLLMMTSESDLTAIREQAQRELAPYRRRMTAVQIRQIEQQFIHKRLLEKYKLPR
                     LSLFYMEQR"
     gene            8805..10082
                     /gene="rlmD"
                     /locus_tag="VFA02_09680"
     CDS             8805..10082
                     /gene="rlmD"
                     /locus_tag="VFA02_09680"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZU10164.1"
                     /translation="MKLRIEKCIYGGAGLARADGKAVFVPFTLPGELVEARLVENKQS
                     FANAELDAVLEPAAERASPHCPYFGECGGCHYQHAGYAQQLEIKRGILRETLERAHLN
                     SLPEIVSVSANPLGYRNRIRLHIAQQPFALCYKRRASRHDLPVNQCPISAPLLEQALA
                     AINRIGADLGLDHFTEIELFTNQAGDELLLSLWASRQMRDCAQVLNRFCSTLHQDIPS
                     LTGAAVFSSGVKHQSRKIAAWQAQWLTCRIAGHEYRVSIGSFFQVNRFLLDKLVELVC
                     GGRAGQLAWDLYAGVGLFSAVLAKRFEQVIAVESAASSSQDLRSNLRGSKHKAVADHT
                     LAFLRQQLRQKFTPPDLIVVDPPRTGLGKEIASLLTKVRAKTLVYVSCDPATLSRDLK
                     ALVESGYRFKNIHMLDMFPQTFHLESVTVLSLD"
     gene            10086..12722
                     /locus_tag="VFA02_09685"
     CDS             10086..12722
                     /locus_tag="VFA02_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZU10165.1"
                     /translation="MPQNAVEPLRFAHLPLFFAAVCFSFGVLISRHFWTNPALLFIAV
                     ILCAVISALAVYKALRPTLLPVAAMFFLLGAFCEEVRPFPDPQTALTTLADGTERVVE
                     GEVSMTSAVRHVESALPFSNKTRQEQSQQIQLQIASVSSNEGTLLPVTGGLSLTLYAP
                     PDAVFPTISCGDKLRVPVSLRLPDHYLDPGVWDERAWLLGQGIGAIAGAKLNAVIITQ
                     KAVRTSFPCWLHSLQQRASSRLIAFAGDAPEGFFRLNHQDATMLTAMLTGDRSYLSRG
                     VRLGFERTGAFHLLVVSGMHLAIFAGLVFFLARALRIPRPAATFATVVIAFAYALFTG
                     YGQPVQRSFWMITLYLTGRLLWRERSAMNSIGFAALGLLVANPDALFDAGFQMTLLTV
                     LATAGIAVPVAEKSFAPYLRAMRNLWTLQIDPSLPPRVAQFRVSLRMMVQHACLLPGA
                     YAAEIIAPATALFLRFLELLLVSVIIEMAMALPMALYFHRITATGIPVNLLIVPLLGV
                     LLPLAMLTLLAIVFLPAVAFVPSAMTAAILHLAIFPVEKFSRSAAGDLRLPTPSIFVV
                     IAWMVCVAFAIWIVRQQRFSVSTALLSLFLGAAVILGWRKSSEAPGRIEITALDVGQG
                     DCILVVSPQGKTLLIDAGGIVGASPDAKFDIGEDVVSQTLWARGIRRLDAVAITHAHA
                     DHIGGMPAVLSNFHPAELWVGHNPDSAAYDHVLQEARELGIYLQVHAAGDAFQFGGMQ
                     VRVLSPAPDYVPKATPGNDDSLVLRVSYGNTSALLEGDAEAPSEARMLSPGGLHSDLL
                     KVGHHGSKTSTTPAFLQAVSPSYAVISVGRHNYYGHPKLETLEKLEGAHALTYRTDLL
                     GASTFYLDGRHITTAAWNHAGQ"
     gene            complement(12732..13349)
                     /locus_tag="VFA02_09690"
     CDS             complement(12732..13349)
                     /locus_tag="VFA02_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZU10166.1"
                     /translation="MALFPIFLKMNGRRCLVAGAGEVAESKIQSLLKAGAEVTVVAPQ
                     AKEAVAALANQGSFRWHQREFRPSDLEGMFLVVAATDRPEVNAAVFKEAAKRNVICNA
                     VDDPPNCDFYFPSIVDRGNLQIAISTAGESPALAQQLRQEIDRQLPQDLGPWLDALGQ
                     LRREVLEMMPAGEPRKLLLHELAQRQVCELEECPSRQLARKTAVK"
     gene            complement(13379..15046)
                     /locus_tag="VFA02_09695"
     CDS             complement(13379..15046)
                     /locus_tag="VFA02_09695"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZU10167.1"
                     /translation="MSETAQTVPVKETKAQRMERLKREKNPWEAFNEVREFARQGRAS
                     VVPEWASAYFKWWGIYTQGDGVGAVGGKGGEGLASEYFMMRIGIPNGILRSDQLRVIG
                     EITRKHARNLADITVRQNIQLHWLTIESLPEIVDALSAIGLSPKGACGDVVRNVTGCP
                     LAGIEHDEIVDASPLALEIAHLLIANPDFRNLPRKFKISVSGCAVWCNYPELNDVALT
                     AVRRGNEVGYTLRVGGGLSNEPHLAVKLDAFIRPEQAVDVVRRVAEIFRDQQVLRESR
                     DRARLKYLFIREGWTAESFLSELESRLGYKLDPGVEEHAPEDILRDHVGIHAQKQPGL
                     TYVGASVLRGRISGEQLAAVADLADRYGKGEIRTTIMQNFAIIHVPAEKAVELADELG
                     KIGLHVEGSAFWRGGIACTGTEFCKLAITETKGFLRWLVEEMEERVPEFTEQLKLHVT
                     GCPNGCGQHWIADIGLEGKKAKHDGKMVDAYVFNVGGAVGKHSAIARPTNYRVPAAEV
                     PAALERLLRAYLAERKPDDNLRTYFARHSVEEIRDRLSGAALPAVVA"
     gene            complement(15301..>17157)
                     /locus_tag="VFA02_09700"
     CDS             complement(15301..>17157)
                     /locus_tag="VFA02_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10168.1"
                     /translation="IGETSLPSAGYNTHSLENEIQISNSWVMSPRVVNDMRFEYTRSR
                     SSQTPLSTDPTISVQGAFTTGGSNSGTVRDNQDYFELQDYFTAAAGKHSINFGARLRA
                     TRDANFSDAGTNGLYIYQSLSDYLNHSPQKYQVTIVNNGQYTARATLFDASLFYQDDL
                     KVSPRFTFSYGLRWEAQNYIHDINDWAPRFSLAYALDGGNGKPAKTVLRAGYGWFYQR
                     WGVANTFGSGGSTPFPIFTIRYNQYHPAQQIFIQSFNNSSSGEENTAPTYYQTDSHFH
                     ASLDMQAAIGIDRQLSKAATMNVTYLYSQGIHQYLTNNITAPIWGATDGTYADVPVDG
                     FHAPPLNLYTYQSEGFYRQSQILASANARFRRFSLFGFYMYNNALGNTGGVNSMPLNA
                     HNLKLDYGRMGFDIHQRFVVLGNINAPWAISLSPMVAYNSGTPYDITIGNDLVANNQF
                     NMRPTFAASCSEPGAVNPADYGIHGMPCLNASPAGTNEKIIPYGYGMGPQNVSMNMRV
                     SKVIGIGPRVEGGGASGGGPHGPGGHGRGGFMGLSGNQGGPGRLDQTVSRKYSLTLSA
                     FGTNILNHENLGTPNGTLLSPFFGKSQSLAGGFFGPRTPGNRSFTLQASFNF"
BASE COUNT         3798 a         4811 c         4533 g         4015 t
ORIGIN      
        1 gagaactgtg cattgagcca caatgcgcgt cactgataaa accgctaacg gtttgtgaag
       61 actgcgccga aagccccagt gcccccagcg tcaaaaaacc tgcaactgct aaagcgttta
      121 gtttcatgct gttgctcctt cgttacatgt cagactgaga tgcgaatctg aaaccctcat
      181 aagaatacgt caaagatggc tggcaatgca gtagcgtgcc cgagtttttt tgatgttgct
      241 aaaagaagca aaaacggagg acccgtgggc cctccgttca aagcgtctgt actgtccgct
      301 agaagttgaa agtcaattga aattcagtgg aacgcggacc gccctggtca aaggtaccag
      361 agccgcggcc aacgcgagtc tggttcttga atgagaagcc attgtcccca ttgacatagc
      421 cttcagcgcc agtaccatct gaaccggcat ttgactggtt gaccaatgtt gtcgttgtgc
      481 cgccccagtt tgcaaagttg gcgacgttat acatcgaaac tttaggtacc agagttaccg
      541 actctgaaat aaatttcagg tggaatggat aggacacgct ggcatcgagc gacttaaaca
      601 ttggattctg aaaaacactc gagaatccgt tgtagatcgg ctgttgcaca gcaccgagtt
      661 ctgacagttg agttggattg aatagaccgt tgttaactaa agcgcggcca gctggtgtca
      721 attgtcctgc gtatttgttg ttgtaattaa cgatgaattt tttcaaatcg ctgccactgg
      781 ttccacgcat gaattggcct ggattggtac ccggtgccaa atcgccaata gttccatcgc
      841 cagtaacatc agtcatgaat atttgcgccg tagaaccagc aagagtatcc aatgtgagat
      901 tagacggagc agctgaacgg aaatggccga tcacagcaat ctgcggcccg tgaatgattg
      961 ttgcagcacc accgaatgaa atgttgtgcc tgtgatcgtt ccctccgaaa ccaatgtact
     1021 gggtcgggtt attgtagtca taagctgccc cgctgaagaa ctgatcggaa ccgccagtgc
     1081 tggagcttac aaattttgaa taggcataag aaaattcaaa attggagtct gcaagccctt
     1141 tcagcggatg acgtgcttgc tggcgaaggt tcaactgaaa ggcatcataa cccgaacgtc
     1201 cggcggggaa attaaagaaa ccatttccca tattcggatt tgcgcctgga aaagcagcgc
     1261 cctcatccgg agtaagtccg tattgtgctg ctggcgctcc gcttagatat acattgcccg
     1321 aatccaagcc attattagca aaatcgctta tagtagcgcc attcgcaatt gcatcatcaa
     1381 tatttgtcac cccaaattga gtaagcgtag cgttgatggc attttgcgca gccgtcttgt
     1441 ttaggtattt cgcatcgcca atgtgattta catcaataga ctgggcgata tgcagcgttg
     1501 cctgatggac gtaatcggcc gacagtacag cgccactcca cagctcacgc tgaataccaa
     1561 aattaaagtg aatagaatat ggtgttttga aatttggtga atatgcagcg tcaccacccg
     1621 gaatcgccag ggtatttcca ataaagctgc cattttgtgc agcaccgcca gctttactga
     1681 tctgttggta ttctgcctgt aatttcacaa atttcggacc agatacagcg agcggctctc
     1741 ggcagacgga tgttagcggg tcgccatcaa tattatctgt cgtagatccg tccggaaatt
     1801 taaaaacata tgttccgcca caaaccgtat ggctatagtc attgaaaaga ccagtttgca
     1861 acttgaaagg agtatcgaac tggactgcat tgaagacgat gttctctcgg aaaatgccaa
     1921 ctgcggaacg cacgaccgtt tttccggcgg aatcaagact gtaggtaaat ccaacttgtg
     1981 gggcaaaatc aaagtttggc tgacgaattc gcttcccaag tccgacgcca aactgatcca
     2041 gaatcagttg gtttcctgtg cagggtggat ttgggaaaat ggatgtgttg atggttgagc
     2101 aaggaattgg gtcgatccaa ttgtctgtgc gccccgtgtc tcgcagccag cggattccgt
     2161 aatttacagt caactgcggc tttactttcc aggagtcgct tacgtaagca cctgtccgcc
     2221 aatcatccga accgcccgct ggctgaccaa atgttggctc cggggtaaag taacctaacc
     2281 cattgccaaa gcgtacacca accgaagcat tatagccgtt caacggatca ccaggagcga
     2341 ttgggtttcc attgctgtct ttaatcaggg ctgcagggct gagagaaacg cgtggggcca
     2401 gaccgaaaaa cgaagcgaac cccccttgaa gaatacggtt caggctggcg ccgtaacgaa
     2461 tgttatgcgc acctttggtc cagctgccat cgtaacgaag ttgcttgtct gattgatatg
     2521 tgtcttgagg ggccagatcg ttgggtcctg tataaagtcc ctggctggca tcccgaatca
     2581 aaagtcccgg catcgccagg taaacacccg aacctgtcgc gtccacgatc agattgtgaa
     2641 atttctcata gctgccacgg aagctgtgcg taaaattgcc cttcacgaag tcagcgccgc
     2701 ctgcaatacc ccatgtatta tcgcggttcg cgtaattatc atatgaattg ttgccactgg
     2761 taatagttga attcacatcg taattgatac gcgcgaaata atgaacaccc cagggaccgc
     2821 tgtaatctaa ccggccagtg gaataggtct gcctatacgg tgctgggaca ttcggatact
     2881 tcgccagaat gtcagcaaat acaggcccaa tttgcgcagg gctggattca tcctgtttaa
     2941 tgcgctctgc attggcaaag aagaacaaac gatccttcag aacaggaccg ccaatagatc
     3001 caccaaactg attgcgctgg aaaggagcgt cctgaccatt tactttggca aaaccagtac
     3061 tatggtcctg gaagtcatag aatgcctctc catgaaacgc attgctacct gtgcgagtgc
     3121 taacaagtac ttgtccagtc gagccaattt cacccgatac gtcgttgtta gaacgggaaa
     3181 tctggaactg atcaatagct ccctgcgaca cattgaaaat cgtcgtgcct actgtctcat
     3241 ccgtgatgtc ctgaccatca agaagaatac gagtggtacg ccctgaaacg ccgctgaacc
     3301 caatggccga atagcccgct ttcgttggat cgaaatcctg accgctttgc agcataactc
     3361 cgggttcgag ttgcgcaaga tccaggaaat tacgcccatt aatcggaaga gtctcaattt
     3421 gctgtgctgt gatgacgttc tggacaccag cctgctcggt gttgacctgc actgcgccag
     3481 cattcactgt cacttcttca gtcgccgcac ctaccgccaa cttgtaattg ccattggtag
     3541 ctgtaccagt caggacaact gtactagcaa ccagcttctg gaaccccgaa gcctcgacaa
     3601 ttacctggta actgcctggt gtcaatggac caacagtgta caggccagcc gcatctgtct
     3661 taagcgtttt ggtaaaccca gtttgttgac tggacacggt aatggttgca ttgcctacag
     3721 cagcgccaga actatccgta atgacacctt gaatagatcc accgtttacc gaaatcgcct
     3781 gggcgtttgc gtgatcagag aagaagaaac tgccagccgc agccgcgaac gtaactgcca
     3841 aggctagcga cccgtatctg attcctgtaa tgagttttgc gcgaaaattc tccttcgctt
     3901 ctttgagcat ttgcatacca ctcctcaggg cacaaaagtt acacaaggaa aggcaggcga
     3961 aatgccaaag ttttacgtct tgttaacagg ccaaatatca atggtttaga tttattagtt
     4021 attaattttt gagtaaacaa atatatccat tttttggtag gaacattcca ttcttcataa
     4081 cctactttga aatcatgaaa caataaaccc agcggttcat caaacaaagg agggccgcgt
     4141 tatgaacact ttcaagactg ctcttttaat gacagcgctc acgctcttcc tgctttttct
     4201 gggggagcac ttcggcgggc gtgatggact cgttctggcc tttatctttg ctgccggaat
     4261 gaacttcttc agctacttct tctcagacaa gctggcgttg gccatgtatc gggcccagcc
     4321 gatcacgcgt gaagagttgc cccgcgtcta ccaggtcgtt gagcggctca cccagcgcgc
     4381 gggtctgccc atgccgaaga tttacgtcat cccaacggat tctccgaatg cctttgccac
     4441 gggccgcaat ccgaaacacg cctcggttgc agtcacgcag ggcatcctga acctgctgaa
     4501 tgatgatgaa ctggaaggcg tcctcgcgca cgaactgggc cacgttcgca accgcgacat
     4561 cctcaccagc tcgattgctg ccacactcgc cggggccatt accatgctgg cgcgcatggg
     4621 ctactgggct tcgctctttg gcggatatgg cgacagccgc gatcgcgaac gcggcggagg
     4681 cggcctcgga gcattgttta tgctcattct ggcgccgatt gcagccacgc tcatccaact
     4741 cgccatctcc cgctcgcggg aatacgaggc tgacgccaca ggcgcgcaca tcacaggcaa
     4801 tccctacgcc ctggccagcg cgctggaaaa gctcgacgcc tattcaaagc gcttgcccat
     4861 gcaggcctcg ccctcgaacg cgcatctgtt cattgtgcag ccgctggtca gcgcacgcga
     4921 tttcgccaat ctgttctcaa cccatccgcc aatcccaaag cgcattgagc gcctgattgg
     4981 acgttccagc atgtatggta gctaacagtt gttagttgtc agtccctggc tgaacttggg
     5041 actggcaact ggaaaaatta tcactatgaa gacacttcta cgctctctga ttttgctgct
     5101 gattgtgttg tggctgggtg gggtgatgtt ctttcccatt gtcgcggcaa cggccttctc
     5161 ttccctgccg gacacgcacg ctgccggcac catcgtgggc cattgcctgc gtgtcctgca
     5221 ctatgaaggg atctttgccg gtgcgctcat cattgtgctc ttcgtgattg cataccgaac
     5281 aggcgcatat cttcgccatg tgattgcgcc ggtttttgtc acgctcatca tgctggcatt
     5341 gacggccttc tctcagttct ggatcatccc gaagatggaa ggttaccggc tggctacggg
     5401 gggcgcagtg gatgcagtag ctcctggcga tccaaaccgc gtggccttca acaagctgca
     5461 cgttacctct gagcgggtgg aagaaggagt gctgctcgcc ggaattgtgc tggtggtgct
     5521 ggtcgcctgg gaaagcacgc cgaaaaagcc ctgatttctc aaacagcttt ttgcaacatg
     5581 gagcggacct cggccaacaa ctgctggata cccagcgcgg ccgtgtctaa catctggtcc
     5641 atttggggac gtgcaaacat cttgcgttcg gcggttgcct gcacctctac cagcccgcca
     5701 tcttcggtca tgacgacgtt catgtctact tcagcctgcg aatcttcttc atacgccaga
     5761 tcgagcagaa tagtattctc cacaatgccg atactggttg cggcaaccaa ttgcctcata
     5821 ggagatttcg gcagcgttcc cgcggcaaca agtcggtcaa aggcaatggc catggccgca
     5881 catgcaccgg taatggcagc cgttcgagtg cctccatcgg cctgaagaac atcgcagtcc
     5941 agcacaacgg tgcgttcgcc aagcgcctgc atgtctacta cggcccgaag tgagcggcca
     6001 atcagccgct ggatttcgtg tgtgcgccct ccgatctttc cacgctcagc ctctcggggc
     6061 gtgcgggtca gggtcgcgcg cggcaacatg ccatattctg cggtcaccca tccccgcccg
     6121 ctgttgcgca gccatgcggg cacactctgc tccacagacg cattgcacaa gacgcgggta
     6181 ttgcctagct cgatgaggac agagccttca gcaacagcta cataatgagg agtgaggcgc
     6241 acaggacgca gggcatctgc ggcgcggcct ccctgacgaa aaaatgaaac cggagtcgtc
     6301 tgcatttcct cattattgta cgaagcggaa tgacagaaat gacatcatta gttgtgatgg
     6361 atgacacatt tgatctggca gagatggacc gtgcactcgc cggcactcct tttgccggaa
     6421 agctgcattt cttccccagc attcactcta caaataccta tgccatgcag caggccgagg
     6481 caggcgctcc tgaaggctca gtgtattttg ccgatgagca aacagccgga cgcggacgcg
     6541 gcgcccacca atgggcctca ccccccggtt caggcttgta tgtaagcgtg ttactgcgcc
     6601 cgcagattcc gccggcagag attttatggc tttcgctagc cgccggactc gcagtgcgcg
     6661 aggcagtgag gcaggtcacc gcgctggaat gcgacttgcg ctggcccaac gatctgctgt
     6721 tcggccgccg caaattctgc ggcattctca ccgagttaaa tgccgaggtc acgcggattc
     6781 ggcacgtcgt agtgggaatc ggaatcaacg tacatcagga aaagtttccg cccgggctgc
     6841 acgacattgc tacttcactg cgtatggaga caggacgcga atggccgcgc cagcaactgc
     6901 ttatcgcctt gttacaatcg cttcatcgcg aagtttcagg attgtcccgc gaagctgctt
     6961 ccggcatttt gcagcggctg gaggccgcct caagctgggt ccgaggcaag cgcgtgcgcg
     7021 tagaagaagg cccggggttt tccggagtga cagaaggatt ggacgcgcgc ggatttctgc
     7081 aagtgcgaac ggcagaggga cttcgtacag tctactccgg cggcgtgcgc gaagcctgat
     7141 acagtgagaa aaccatgctt cttgtgctca acgtcaataa tacgaacaca gtcatcgcgc
     7201 tctacacgct caggcaggac ggccttgccg acaagctgcg cgcaacctgg cgcatcagca
     7261 cccggcatgt gcagaccgcc gacgaatacg gcattttggt acgcagcctt ttgcaaagcg
     7321 aaggcgtgga gggtcccgca atcaagtccg tggtcatcgc ttccgttgtg ccgccggtgg
     7381 actgggtgct gcgccagttc tgcgagcgct attttcacca gaagccgctc ttcatcgagc
     7441 ctggtgtcaa aaccggctta ccgattctca ccgacaatcc cagcgaagtg ggcgcggacc
     7501 gcgtcgcgaa ctgtgtggga gcattcgcaa aatatggcgg ccctgcgatt gttgtggact
     7561 tcggcaccgc gacaaacttt gatgttgtct cgcgtaaagg cgagtttctg ggaggggcca
     7621 ttgctcccgg tctcaacgtt tctgccgaag cgctgtttgc gcgcgctgcg cggctgccac
     7681 gcgtggagat caagcggccg gcaaaaatca tcgggacgaa caccgtagac aatttgcaga
     7741 ttggcctgtt ttacggccat atcggcctgg tagattacat tctggaacgt atggtcgcca
     7801 aactcggcga aggcaccaga tgtatcgcta ctggtggcct tgcccacctc atcgcgagcg
     7861 agtcgaagtt tattactgaa atagatgaca tgcttaccct ggacgggctg aggatcattt
     7921 atgaccgcaa tcggtcggca aaggcccatc cacacgccgg tgaaatgcaa cagccatagc
     7981 attttcacag atagtctgaa tcatgcccgg aaactaaagt ctctgcgacc aacagggagt
     8041 cggcgacact gcacagtgca aatggagtgt acagaatctg tgaaaatgcc gtaaaaccgt
     8101 aattgacacc ctacagttgt actcttggaa aattacttca actactttac ggaaatcgaa
     8161 gagcactttc agcggcggcg cggctccctg ctgctgctct ctaccttgga ctgggcattg
     8221 attgagacct ggcgcgaagc cggagtaccg cttgaggccg tcctgcgcgg cattgatgca
     8281 acatttgaaa aatatgaagc acgaaagacc cgcactcgcg tccggcgcat caatgggctg
     8341 gcctactgcg cacaagaggt gatgtccgcg gttgaagaaa tgaaagaagc ggcggccggt
     8401 gcggcgtctg ctctcaatca agcggctccc cctcctggat ttgaaccgga acgcattgcc
     8461 ttgcaccttg agtcctgcgc cgaagcattg cgaaatgtac agataaacga aacggcacag
     8521 cgcctgctgg cattggcctc cggtgtgcgt atgcaacagc cggacctcga agacctggag
     8581 cgcacgctta ctacgctcga agaaaaactc ttcgcccatc ttcttatgat gacgtccgaa
     8641 tctgatctca ctgccatccg ggaacaggcc cagcgtgaac tcgctcccta taggcgccga
     8701 atgacagccg tacagattcg tcagattgag cagcaattta ttcataagcg gctgcttgaa
     8761 aaatataaac ttccgcgcct gagcctcttc tacatggagc agcgatgaag ctgcgcattg
     8821 agaagtgcat ttatggagga gccgggctgg cgcgcgccga tggcaaggcc gtctttgtgc
     8881 cgttcacatt gccgggcgaa ctggtagaag cgcgcctcgt ggaaaacaag cagagttttg
     8941 cgaatgcaga acttgatgca gtgctggaac cagcagcaga gcgcgcttct cctcactgcc
     9001 cgtattttgg cgaatgcggt gggtgtcatt atcagcatgc cggctatgca cagcagttgg
     9061 aaatcaagcg tggcattctc agggaaactc tggagcgtgc tcatctgaat tccctgccgg
     9121 agatagtttc tgtttctgcg aaccctctgg gatatagaaa ccgcatacgc ctgcatattg
     9181 cgcagcaacc ctttgcgctt tgctataaac ggcgtgcttc gcgccatgac ctgccggtca
     9241 accagtgccc catctctgcg ccgcttctgg aacaggcgct ggctgcaatc aatcgcatcg
     9301 gagcagatct cggcctggac cacttcacgg aaattgaact cttcaccaat caggccgggg
     9361 atgagttgtt gctttctctc tgggccagca ggcagatgcg ggattgtgcc caggttctca
     9421 accggttttg ctcgacgttg catcaagaca ttccatcgct caccggcgct gctgttttct
     9481 catccggtgt aaaacaccaa agcagaaaaa tcgcggcatg gcaggcgcag tggcttacct
     9541 gccggatcgc gggtcatgaa tatcgcgtca gcatcgggtc tttctttcag gtcaaccgct
     9601 ttcttttaga taaattagtc gagcttgtct gcggcggacg ggccggacaa ctggcatggg
     9661 atttatacgc cggtgttggg ttgttctcag ccgtacttgc aaagcgattc gaacaggtca
     9721 tcgcagttga atccgccgcc tcatcttctc aggacttgcg cagcaatctc aggggaagca
     9781 aacataaagc tgtcgctgat cacacgttgg cttttctgcg ccaacagctc cgccagaaat
     9841 tcactccgcc tgatcttatt gtcgtagacc ctccccgcac tggactcggc aaggaaattg
     9901 cctctctgct tacgaaagta agggccaaaa cgctggttta tgtctcatgt gacccggcaa
     9961 cgctttcgcg cgacctgaaa gcattggtag aatccggcta tcgcttcaaa aatatacaca
    10021 tgttagatat gtttccccag acattccatc tggaaagtgt cacggtgctg tcgcttgact
    10081 gaagtatgcc tcagaatgct gtagaaccgt tgcgctttgc gcatctccct ctattctttg
    10141 ctgccgtctg cttttccttt ggtgtcctca tttcccggca cttctggacc aaccccgcgc
    10201 tgctgttcat cgcggtcatt ctgtgcgcgg tcatctctgc tttggcggta tataaggcgc
    10261 tgaggcccac tttgcttccg gttgccgcaa tgtttttcct tctgggagca ttctgcgagg
    10321 aagtaaggcc gtttcccgat ccacagaccg cactcacaac gcttgccgat ggaacagagc
    10381 gggttgtcga gggcgaagtt tccatgacta gcgccgtgcg gcacgtcgag tcggctttgc
    10441 cgttttccaa taaaacacgt caggagcaga gccagcagat tcaactgcaa atcgcttccg
    10501 taagttcaaa tgaaggcaca ctgttacctg tcaccggtgg actatcactc acgctctatg
    10561 ctccgcctga tgcagtcttc cccaccattt cctgcggcga caaactccgc gtccccgtct
    10621 cgttgcgcct tccagaccat tacctggatc ctggcgtgtg ggacgaacgc gcgtggttgc
    10681 ttggtcaggg catcggcgca attgcaggcg ccaaactgaa tgcggtcatc atcacccaaa
    10741 aggcagtgcg cacttcattc ccgtgctggc tgcattctct gcagcagagg gccagctcgc
    10801 gtctgattgc atttgccggg gatgcgccag aaggcttctt ccgcctcaat catcaggatg
    10861 ccaccatgct taccgccatg ctcacaggag accgcagcta cctctcgcgc ggagttcgcc
    10921 tgggctttga gcgcaccggg gccttccatc tgctggttgt ttccggaatg catctggcca
    10981 ttttcgccgg acttgtcttt tttcttgcgc gcgcgctgcg tattccccgc cctgccgcaa
    11041 catttgccac ggttgtcata gcgtttgctt atgctttgtt cacaggatat ggccaaccgg
    11101 tacagcgctc cttctggatg atcacgctct acctcactgg ccgtttgcta tggcgcgaac
    11161 gcagcgccat gaactcaatc ggatttgcag cgctgggcct gctggttgca aacccggatg
    11221 cactgtttga tgcaggcttt cagatgaccc ttctgacagt gctggccaca gccggaatcg
    11281 ccgtgcctgt cgcagagaag tccttcgccc catatctgcg ggccatgcgc aatctgtgga
    11341 cgctgcagat tgacccatct ttgccgccgc gtgtcgcaca gttccgcgtc agcctgcgca
    11401 tgatggtgca acacgcgtgc cttctgccgg gcgcatatgc cgctgaaatc atcgctcctg
    11461 caactgcctt gtttctgcgc ttcctggaac tgctgctggt ttcagtcatc atcgagatgg
    11521 ccatggcgct gcccatggcg ctgtactttc atcgcatcac cgcaacaggc attccggtaa
    11581 acctgctgat tgttccgctt ctgggagtgt tgctccccct cgccatgctg actcttctcg
    11641 caatcgtatt tctgcctgca gttgcttttg ttccctcagc gatgaccgca gccattctgc
    11701 atctggcgat ttttcccgta gagaaatttt ctcgctccgc tgcaggcgat ctgcggctcc
    11761 ccactccttc gatatttgtc gtgatcgcat ggatggtttg cgttgcattc gccatctgga
    11821 tcgtgcggca gcaacgtttt tcagtgtcca cggccctttt gtcgttgttt ttgggggctg
    11881 cagtcattct cggctggcgc aaatcttcag aagcccccgg gcggattgaa atcacagcac
    11941 tcgacgtcgg ccagggcgac tgtatcctgg tggtctctcc gcaaggcaaa acactgctca
    12001 tagatgcggg tggaattgtg ggcgcatcgc ccgatgcaaa gttcgacatc ggggaagacg
    12061 tggtttcaca aacactctgg gcgcgcggca ttcggcggct ggatgcggta gccatcactc
    12121 atgctcacgc cgaccatatt ggtggaatgc cggcggtact ctcaaatttt catcctgcag
    12181 aactttgggt cggtcacaat cccgacagcg ccgcttatga ccatgttctc caggaagcca
    12241 gagaacttgg aatctacctg caggtacacg ctgcaggaga cgcttttcag ttcggaggaa
    12301 tgcaggtgcg cgtgctctct cctgctccgg actacgttcc caaagcaacg cctggcaatg
    12361 acgattcgct tgtccttcgt gtgagttacg gcaacacttc agccctgctc gaaggcgatg
    12421 cggaagctcc cagcgaagcc cgtatgttgt cccccggggg gctacattcg gacctgctca
    12481 aagtcggaca tcacggcagc aaaacatcta caacgcccgc ctttcttcag gcagtctcac
    12541 catcgtatgc ggtcatttct gttggacggc acaactatta tggtcacccc aaactcgaaa
    12601 cgctggagaa actcgaaggc gcacatgctc tgacgtaccg cacagatctg ctgggtgctt
    12661 ctacttttta tctggatggc agacatatca caactgccgc ctggaaccat gccggacagt
    12721 aaaactcgac ctcacttaac ggccgtttta cgcgcaagct ggcgtgacgg acactcttcc
    12781 agttcgcaca cctggcgctg ggcaagttcg tgcagcaaca gcttacgtgg ctcgccggca
    12841 ggcatcatct ccagcacctc acggcgaagc tgtcccaaag catccagcca agggcctaag
    12901 tcttgtggaa gctgcctgtc tatctcctga cgcaactgct gggccagagc aggactttct
    12961 ccggcagtgg aaatcgcaat ctgcaggttc ccgcggtcca cgatggaagg gaaatagaag
    13021 tcacagttcg gagggtcgtc cacagcattg cagatgacgt tgcgctttgc tgcttcttta
    13081 aaaacagcgg cattgacttc aggacggtct gtcgcggcaa ccaccagaaa catgccctca
    13141 aggtccgagg gccggaactc tcgctgatgc cagcgaaagc tgccttgatt tgccagggcg
    13201 gccacggctt ccttggcttg aggcgcaaca acagtcacct ccgcccccgc ctttagaaga
    13261 ctttgtattt tcgattccgc aacttcccct gcgccggcca caagacatcg ccggccattc
    13321 atcttgagga agataggaaa aagcgccatt cagttatttt acgtaaagtt cccctgcgct
    13381 atgccactac agcaggaaga gccgcaccgg agagccggtc acgaatttcc tcaaccgaat
    13441 gccgcgcaaa gtatgtgcgc aggttgtcat caggctttcg ttcggcaaga tacgcgcgca
    13501 gcagccgctc cagcgccgcc ggaacttctg ccgccggaac gcggtagttt gtgggccgcg
    13561 caatcgcgga atgtttccct acggcccctc caacattaaa tacatatgcg tccaccatct
    13621 tgccatcatg cttggccttt ttgccttcga ggccaatatc ggcaatccag tgttgcccac
    13681 aaccgttggg gcatccggtg acgtgcagct tgagctgctc ggtaaattcc gggacccgtt
    13741 cttccatctc ttcgaccagc cagcgcaaaa agcccttcgt ttccgtaatg gccagcttgc
    13801 agaattccgt cccggtgcag gcaatgcctc cacgccagaa ggccgaacct tccacatgca
    13861 gaccaatctt acccagttcg tccgccaact caacggcttt ttccgcaggc acatggatga
    13921 tggcaaaatt ctgcatgatg gtcgtgcgga tttcgccttt tccatagcgg tccgcaagat
    13981 cggcaactgc ggcaagctgt tcgccgctga tgcgcccgcg aagcactgaa gcccctacat
    14041 aggtcaaacc aggctgtttc tgcgcatgaa taccaacatg gtcacgcaga atgtcttccg
    14101 gtgcgtgctc ttccacacca ggatccagtt tgtacccgag gcgggactcc agttcactta
    14161 gaaagctctc tgccgtccag ccttccctta tgaaaagata cttcagacgc gcacggtcgc
    14221 ggctttcgcg aagcacctgc tggtcccgga aaatctccgc tacccgccga acaacatcta
    14281 cagcctgctc aggacggatg aaggcatcca gtttgaccgc gaggtgcggc tcattggaga
    14341 gtccgccgcc aacgcgcaga gtatagccaa cctcatttcc gcgacggacc gccgtcagag
    14401 caacatcgtt cagttccgga tagttgcacc acacggcaca tcccgaaacc gagattttga
    14461 atttgcgcgg aaggttccgg aaatccgggt tcgcaatcag cagatgggca atctccagtg
    14521 ctaatggaga agcatccacg atctcgtcat gctcgattcc tgccaacggg catcctgtca
    14581 cgttgcggac cacgtcgccg caggctcctt tgggagacag accgatcgcg ctgagcgcat
    14641 ccacaatttc aggcagcgat tcgatcgtga gccagtgcaa ttgaatgttc tggcgcaccg
    14701 taatgtcggc gagattgcgc gcatgcttgc gggtaatctc gccgatgacg cgaagctggt
    14761 ccgaacgaag gatgccattg ggaatgccaa tgcgcatcat gaagtactct gaggccagcc
    14821 cttcgccccc tttgcctcct acagcgccca ctccatcgcc ctgggtatag ataccccacc
    14881 atttgaagta ggctgaggcc cattccggta caacgctggc ccgtccctgt cgcgcgaact
    14941 cgcgcacttc gttgaatgct tcccaggggt tcttctcgcg cttcaggcgc tccatcctct
    15001 gggccttggt ttcttttacc gggactgtct gggccgtctc gctcatcgta ttcctgtagt
    15061 gaaatttggg aataaaaaaa ccgcgtcagg tggtcctgcg cggggtcttg tgactgctga
    15121 agctctggag gtgctggagt cattcgctca caagtccggc gcttacgcaa caacatgcgc
    15181 aacatgcggc cggagtcgtg tgggaacgaa acattctctt attgcaagat tatcgggttt
    15241 tgggcgggag cgtcaagaag aagcagtgct ccgcatacct tctgcggagc accataaaca
    15301 tcagaagtta aaggatgcct ggagggtaaa gctgcggttt cccggcgttc tgggcccgaa
    15361 aaagccgcca gcaagcgatt gggactttcc aaagaacggc gaaagcagtg tcccatttgg
    15421 cgtacccagg ttctcgtggt tcaggatgtt ggtcccaaag gccgagagcg tgaggctgta
    15481 cttgcgcgag accgtctggt ccagccggcc tgggccgccc tgatttccac tcaggcccat
    15541 gaacccgccg cgcccgtgac ctcctggccc atgcggccct cctccactgg ctccaccacc
    15601 ttccacgcgc gggcctatac caatgacctt gctgacgcgc atattcatgc tgacgttctg
    15661 tgggcccatg ccatacccat agggaatgat tttttcattt gtgcctgccg gactggcatt
    15721 caggcacggc attccgtgta tcccataatc tgccggattg acggctccag gttcagaaca
    15781 gcttgctgca aaagtaggac gcatattgaa ctggttgttc gccaccagat cgtttccgat
    15841 ggtgatgtca tagggggtcc cggaattgta cgccaccatt ggagagagag agatggccca
    15901 cggcgcattg atgttcccca gaactacgaa ccgctgatgg atgtcaaagc ccatgcgtcc
    15961 ataatcaagc ttcagattat gcgcgttcaa tggcatggag ttgacacctc cggtattgcc
    16021 aagcgcgttg ttgtacatat agaagccgaa gaggctgaag cggcggaagc gcgcattggc
    16081 gctggcaaga atctggctct ggcggtagaa gccctccgac tggtacgtat acagattgag
    16141 tggcggtgca tgaaatccgt ccacaggcac gtccgcgtag gtaccatcgg ttgcgcccca
    16201 gatgggcgcg gtgatgttgt tggtcagata ctgatgaatg ccctggctgt aaagataggt
    16261 aacgttcatc gtcgcagctt tactcaactg ccggtcaatc ccaatggccg cctgcatgtc
    16321 gagagaggcg tggaaatgcg aatctgtctg gtaataggta ggcgcagtgt tttcttcgcc
    16381 cgagctggaa ttattaaagc tttgaatgaa gatctgctgc gccgggtgat actgattgta
    16441 acggatggta aagattggga aaggcgtgct gcctccacta ccaaaggtgt tggccactcc
    16501 ccagcgctgg tagaaccagc cgtatccggc ccgcaggact gttttggccg gcttaccgtt
    16561 accaccatcg agcgcgtaag ccagagagaa ccgcggtgcc cagtcattga tgtcgtgaat
    16621 atagttctgt gcttcccacc ggagcccata gctgaacgta aagcgtgggc ttaccttcaa
    16681 gtcatcctgg taaaacaacg atgcgtcaaa aagcgtcgcg cgtgccgtgt actgcccgtt
    16741 gttgacgatg gtcacctggt acttttgcgg gctgtgattg aggtaatcag acagcgactg
    16801 gtagatatag aggccattgg tacctgcatc gctgaagttc gcatcgcgag tagcacgtag
    16861 ccttgcgccg aagtttatgg agtgtttccc ggcagcggcg gtgaagtagt cctgcagctc
    16921 gaaatagtcc tggttatcac gaacagtccc gctgttgctg ccgcccgtag taaaggcgcc
    16981 ctgtactgag atcgttgggt ccgtggacag cggggtctgt gacgagcggc tgcgcgtata
    17041 ttcaaagcgc atgtcattta caacccgggg actcatcacc caactgttgc tgatctggat
    17101 ctcattctca aggctgtgag tgttatatcc agccgaaggc agactggttt cgcctat
//
LOCUS       DASRBV010000265         8315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_118433, whole genome shotgun sequence.
ACCESSION   DASRBV010000265 DASRBV010000000
VERSION     DASRBV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118433"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFA02_09705"
     CDS             complement(<1..221)
                     /locus_tag="VFA02_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10169.1"
                     /translation="MNRTRWHLPLLSALVLSLCLSAKAQSLYQQFLHPPDDARVMMRW
                     WWFGPAVENNEIERELHAMKDGGIGGVEI"
     gene            complement(251..532)
                     /locus_tag="VFA02_09710"
     CDS             complement(251..532)
                     /locus_tag="VFA02_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10170.1"
                     /translation="MEEREFFNETHEQRAHILTCPHCGKAEEYQLGWIVRQKKASLPR
                     HADERDRARFAKAQSYMVRKDDMVACKNIRCRKRFEVAGVQSVAFLQSA"
     gene            593..763
                     /locus_tag="VFA02_09715"
     CDS             593..763
                     /locus_tag="VFA02_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10171.1"
                     /translation="MAKKKTKKFSAVKAVKANARERVGQPKPERIIEEKPRTESRKAK
                     HKPKLTDLLIEE"
     gene            complement(774..1958)
                     /locus_tag="VFA02_09720"
     CDS             complement(774..1958)
                     /locus_tag="VFA02_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZU10172.1"
                     /translation="MLYRFVLIVSLALSAFSQQSPLPQTALERLFKQVPASPSWFDTN
                     FLAQVPIERVNAIIQQYTQQNGAFQKATANPDGSYTVTLAHATVPARIVLNAEGRITG
                     LWFGYPAPIQAISLDTVIAQFARLPGKVSVLVTEENKPLASLHSDEALAVGSAFKLAV
                     LNAVSDQVRRGKHHWADVVPLNPAWKSLPSGVLRQWPDNTPLTLATLANEMISISDNT
                     AADALLSLAGRNNVEAFAPRNKPFLSTREAFTLKSTANAALLTRWRNADETGRRALLK
                     EIDAKPLPGEAEVTASPIALDVEWQFTTSELCNLIAGVAGNPAMHINPGLAQASDWKQ
                     VAYKGGSESGVLNMTTSMIGKNNKRYCAAATWNNTSPLDEKAFFSFYSELLSTLAGGA
                     LK"
     gene            2132..2977
                     /locus_tag="VFA02_09725"
     CDS             2132..2977
                     /locus_tag="VFA02_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZU10173.1"
                     /translation="MDLKISPVLRQIALYSLFCLAAMGLYAQDTQNDDCSLHNLKPCV
                     VHVAQDEVGIITSPLRMNSSSLVWVAPFGLATGLSIDYDAHMMRKLGVHPDREDKFRK
                     FSDYAGLYGPTAAPVIGYFVGAGTHNDYLRESSVLAGEATLDAVILNEGLKYAIDRER
                     PNQGDGTGRFWPHGTRTWPDGQSMPSNHCMIAWSFARVVAGQYHTPATQIAVYSLAAG
                     VSASRVLSREHFPSDVIVGSTFGYLIGDYVFRHRSREASLFSLSAVNTANGRGMQLNI
                     NLGRR"
     gene            complement(2983..3321)
                     /locus_tag="VFA02_09730"
     CDS             complement(2983..3321)
                     /locus_tag="VFA02_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZU10174.1"
                     /translation="MDCIFCKIIAGNIPSKKVFEDEQVYAFADIDPRAPVHILIVPKK
                     HIPSLAAADASDMPLLGHIHAMAAKLAQEKGLANGFRTVINTGPDGGQTVSHLHLHLL
                     GGRQMHWPPG"
     gene            3394..4062
                     /locus_tag="VFA02_09735"
     CDS             3394..4062
                     /locus_tag="VFA02_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU10175.1"
                     /translation="MRSRFSMFSAVIAALVAFAPARAQFSNPQTEMTPVHNARALRPP
                     AGARVAIFEFEDLECPDCARANPLLKEASEKYHIPWVRHDFPLPQHTWSFDAAVDARW
                     FDTKSKKIGDDFRDAVFANQPSIATQDDLRNFAQKFAQQHNLAFPFVVDPQGKLAALV
                     KADYDLGQSIGIQHTPTIWIVTNKTSGVPFVEVVDRNRLYELIDQALAETKNETGTHH
                     SSGK"
     gene            4160..4456
                     /locus_tag="VFA02_09740"
     CDS             4160..4456
                     /locus_tag="VFA02_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZU10176.1"
                     /translation="MPLYEYQCKACGYVFETIKSFSDPDEKECPVCKGEVERLLSAPA
                     VQFKGSGWYVTDYAGKGGSKKAVSGPGESNGSKPVEKSSVSESSASSSASSKSD"
     gene            complement(4458..5057)
                     /locus_tag="VFA02_09745"
     CDS             complement(4458..5057)
                     /locus_tag="VFA02_09745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10177.1"
                     /translation="MTGLPWDASYLNGAAPWDIGQPQPAIARLASEGGFMGPVLDVGC
                     GTGENTLLIASLGLQVVGMDVAETAIAIAREKAKNSGLDIEFAVADAFHLNQLRRSFD
                     TVLDCGLFHTFNSEERTEYLASLASAIKHNGTLYVLCFSDDGPGTGPHPVRQEELKAA
                     FNPGTGWNIVAIRPERILTRYRDRNGAPAWLATMKVLDI"
     gene            complement(5381..7783)
                     /locus_tag="VFA02_09750"
     CDS             complement(5381..7783)
                     /locus_tag="VFA02_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10178.1"
                     /translation="MTRDLRLSLRMIFSHRWFSAAIVATLALGIGLNTMIFTLVNAAL
                     MKSVPVAGGERLVAINSRNVTRGPDRSRVSYPDFLDYRVQATSFESLNAAFNDEGVLS
                     ENSNPPQAYSLERASSGIFSSLNIHPILGRAFLPTDDKPGAEPVLVLGYGVWKERYNS
                     SPSVIGRQVRVNGRPATIVGVMPKGFKFLTNTDIWMPLTPTPELEKRDNRPLQLFGIL
                     KPGTTVTEAQAELNQIADRLAKQYPVTNKDIETSVETFQQHYNGGNIRMVFLLMLAAV
                     GFVLLIACANVANMMLGRALVRQREMSIRAALGASRWLIVRQLLMESVLLSIFGGILG
                     LALAAFGVHWFDLSTQNVGKPYWVEFTMDYAVFGYFAVLCIFCGLLFGMAPALRSSRV
                     DLNEVLKEGARSAGKHRGGRLSGLLVIFQLALTLVLLTGAGFFVQSLLKAFSANRDVP
                     ADQLMTARLDLPDGHYKDTDARQRFYNQLLPRLKAIPGVSHAALVSDLPGLGANTSQI
                     EIEHSTIDNPAHRPAVSYLVQGPGYFATINLPLLQGRDFNETDGTANHKAAIVTREFA
                     QHFWPNQNAIGKRFRFYDEKNNPGDWLTIVGVSANLVQQLDDVHPKPLLFLPYREKGW
                     SSMALMVRSNTNPINAVRAAVQGLDQDLPLREVYTFPKALDHQLWYLHLFSKLFAGFA
                     LIALLMASVGIYAVIAHATNARTQEIGVRMALGANARNILMLVMRRGLWQITAGLTLG
                     MAAAVPAARLIGSLPIGITPSDPLLFAIVALLLAVVCLFACWLPAHKAAALDPVKAIR
                     YE"
     gene            complement(7847..>8315)
                     /locus_tag="VFA02_09755"
     CDS             complement(7847..>8315)
                     /locus_tag="VFA02_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10179.1"
                     /translation="LELVLGLNLAGALKAVISGVSLTVIGTLLAGLGVIFHPITLLQL
                     SLLILTTSIAFNGMMFLLMVRVEDPLVPRAMFGVLNTLLFFPSGAISPISGFPKWLRA
                     IAVVDPFTYAVHGFKAILLKDGGFAAIWSDLLFLLGFGVVALLIATPLFKRTL"
BASE COUNT         2012 a         2202 c         2291 g         1810 t
ORIGIN      
        1 tgtatctcta cgccgccaat gccgccatct ttcatcgcgt gcagctcgcg ctcgatctcg
       61 ttgttttcaa ccgcggggcc gaaccaccac cagcgcatca tcacgcgcgc gtcatcagga
      121 ggatgcagaa attgttgata cagcgactgc gcttttgcag aaaggcatag agacaaaacc
      181 agagcagaca gcaatgggag atgccagcgg gtacgattca tcagaaaagt ttcgcctcag
      241 aatgtaaagt tcaggccgac tgtaaaaagg ccacggactg cactcccgct acttcaaacc
      301 gcttccggca acggatgttc ttgcacgcca ccatatcgtc tttgcgcacc atataggatt
      361 gtgccttagc gaatcgcgcg cggtcacgct catcagcatg gcgtggcagc gatgcttttt
      421 tctgccggac gatccatcca agctggtatt cctcagcttt gccgcagtgc ggacaggtaa
      481 gaatatgggc ccgctgctca tgagtctcgt tgaaaaattc acgttcttcc atggcaacct
      541 ccctatgact gacgatgaaa gtatttcata ctggaaacaa aagagggcgc tcatggccaa
      601 aaagaagaca aaaaaattct cggcggtgaa ggcagtcaaa gcaaatgcga gggagcgtgt
      661 aggccagccc aagcctgagc gtatcatcga agaaaagccg cgcacggagt cccgcaaggc
      721 gaaacacaag ccgaaattga ccgatcttct tatcgaagag taagtagcag actctacttt
      781 aaggccccgc ctgcaagtgt ggacagaagc tccgaataaa aggagaaaaa cgccttttca
      841 tccagcggag atgtattgtt ccaggtggcg gccgcacaat aacgcttatt gttcttccca
      901 atcattgaag ttgtcatatt cagtacgccg ctttcagagc cgcccttgta ggcgacctgc
      961 ttccagtccg atgcctgcgc aaggccggga ttgatatgca tcgcaggatt gccggccaca
     1021 ccagcaatca aattgcagag ttcgcttgtg gtaaattgcc actccacatc aagcgcaatc
     1081 ggagacgcag tgacttcagc ttcgccggga agcggctttg cgtctatttc tttcagcagc
     1141 gcccgcctgc cggtttcatc tgcattgcgc caccgcgtca gcaatgccgc atttgcagtg
     1201 gacttcaggg taaaggcctc gcgggtagag aggaacggct tgtttcgcgg agcgaaggct
     1261 tccacattgt ttcgcccggc aagcgagagc agcgcatccg cggccgtgtt atcgctaatc
     1321 gagatcattt catttgcaag tgtcgccaga gtcagcggag tattgtctgg ccattgccgc
     1381 agcactcctg aaggcagcga tttccatgct ggattcagcg ggacaacgtc tgcccagtgg
     1441 tgtttgcccc tgcgcacctg gtcactgact gcattcagaa ccgcaagctt aaatgcagag
     1501 ccaacggcaa gcgcttcgtc tgaatgcagt gacgccagag gcttgttctc ttccgtaacc
     1561 aggacagata ctttccccgg aagcctggca aactgcgcga tgaccgtgtc cagactgatg
     1621 gcctgtatag gcgctggata accgaaccag agtccggtga tgcgtccttc tgcgtttagc
     1681 acaatgcgag caggcacagt ggcatgtgcc agcgttacgg tgtaagagcc gtcaggattt
     1741 gctgtggcct tttgaaaggc cccgttttgc tgcgtgtatt gttggattat cgcgttcacc
     1801 cgctcaatgg gtacctgtgc caggaaatta gtgtcaaacc aggatggaga cgcagggact
     1861 tgcttgaaaa gccgttccag cgcggtctgc ggcaaaggag attgctggct gaaggcagaa
     1921 agagcaagcg aaacgatgag cacaaacctg taaagcatag aattttcctc gcgcaagacc
     1981 cagcaacatt ggagcatatt tgccgtgagc ggagagggag ttccagggga tctgcagaaa
     2041 agggcatgac gtgggcggaa ctatgcgctt gtctagcagt cattccgcaa tcaaattctt
     2101 gtatgatgcg aaataaacct ctggaatttg aatggacctg aaaatttctc ctgttcttcg
     2161 gcaaattgcg ctgtattcac tattttgcct tgccgctatg gggctttatg cacaggacac
     2221 acagaatgat gactgctctc tgcataatct gaagccctgc gtggtccacg tcgcgcagga
     2281 cgaggtggga atcatcacca gtcccttgcg gatgaacagc agcagcctag tttgggtcgc
     2341 cccctttggt ttggcgacgg ggctttccat tgattacgac gcgcacatga tgcgcaaact
     2401 tggtgtccat cccgaccgcg aagacaagtt caggaaattt tcagactatg ccgggctgta
     2461 tgggccgacg gctgctcctg tcattgggta tttcgttggt gccggaacgc acaatgatta
     2521 tttgcgcgaa tcgtcagtgc tcgcaggtga agcaacgctg gatgcggtca ttctcaatga
     2581 aggtctgaaa tacgccattg atcgcgagcg tccaaaccag ggagacggca cggggcgatt
     2641 ctggccgcat gggacaagga cctggcccga tggccaatcc atgccctcga accattgcat
     2701 gattgcttgg tcgttcgcgc gcgttgtcgc cgggcagtat cacacgccag ccacacagat
     2761 tgccgtgtac tcattggcag caggggtttc agcttcccgc gtcctctcac gcgagcactt
     2821 cccctctgac gtgattgttg gcagcacttt tggctacctc atcggagact acgtcttccg
     2881 ccaccgttcg cgagaggcca gtctgttttc gctgtccgct gtgaatacgg ccaatgggcg
     2941 tggaatgcag ctcaacatca atctgggcag gcgatgattt ttctatcccg gaggccagtg
     3001 catctggcgc cctcccaaca ggtgcaggtg caggtgggaa actgtctggc ccccgtccgg
     3061 gccggtattg atgaccgtgc ggaagccgtt ggcaagccct ttttcctgcg caagctttgc
     3121 tgccattgcg tgaatatggc ccagcaacgg catgtccgac gcatcggcgg cggccagcga
     3181 cgggatatgt ttcttcggca caatcagaat atggacgggc gcgcgcggat caatatcggc
     3241 aaaagcatag acctgctcat cttcaaatac cttttttgag ggaatgttcc cggcgatgat
     3301 cttgcagaag atgcaatcca tgcggacaga atatcgctgt ttaccgtgtt tttgcggctg
     3361 tccgataaaa taaaggtaac gagtccttta tctatgcgat ccagattcag catgttttcc
     3421 gccgtgattg cggcgcttgt ggcctttgcg cccgcgagag cgcagttttc caatcctcag
     3481 accgagatga cgcccgtgca taatgccaga gcattgcgtc cacctgccgg agcgagggtt
     3541 gcgatttttg aatttgagga cctggagtgt cccgactgcg cgcgcgccaa tcccctgctg
     3601 aaggaagcct cggagaaata tcacattccg tgggtcagac atgattttcc tctgccgcag
     3661 catacgtgga gcttcgacgc tgccgtagat gcgcgctggt tcgatacgaa atcaaagaag
     3721 attggcgatg acttccgtga tgcggtcttt gccaaccagc ccagcattgc tacgcaggat
     3781 gatttgcgca atttcgctca gaagttcgca cagcagcaca atctggcgtt tccgtttgtg
     3841 gtggaccccc agggaaagct ggccgcattg gtaaaggccg actatgacct ggggcagagc
     3901 atcggaatcc agcacacgcc gaccatctgg attgtgacga acaagaccag cggcgttccg
     3961 tttgtagaag tcgtagaccg caaccgtctg tatgaactga ttgatcaggc gcttgcggaa
     4021 acgaagaacg aaacggggac acatcattcc agcggaaaat gagaacggat tttgatcggc
     4081 tgcgggcgaa gtccagaaag cagttcttag cttgcggcct tgaatgacaa cttgatgatt
     4141 gcaaggagta agtaagatcg tgcctttata cgaatatcaa tgcaaagcct gtgggtatgt
     4201 ctttgagaca atcaagagtt tcagcgatcc cgatgagaaa gaatgcccgg tgtgtaaggg
     4261 tgaggttgag cgactgctct ccgctcctgc cgtgcagttc aagggttcgg gatggtatgt
     4321 gaccgactat gccggcaagg gcggaagcaa gaaagctgta tcgggtccag gtgaaagcaa
     4381 cggcagcaag cctgttgaga aatcctctgt atcggagagc agcgcctcca gttctgcatc
     4441 ttccaagagc gactaatcta aatatctaat actttcatcg tcgcgagcca ggctggcgcg
     4501 ccgttcctgt cgcgatatct cgtcaggatt cgctccggcc tgatagcgac gatattccat
     4561 ccagtgccgg ggttaaatgc ggccttcagt tcttcctggc gaacggggtg cgggccagta
     4621 ccaggaccat catcactgaa acacaataca tacagggtgc cattgtgttt aatcgctgat
     4681 gccaggcttg ccagatattc ggttcgttct tcactgttga aggtgtggaa cagcccgcag
     4741 tccagcacag tatcaaaact tcgcctcagt tgattcagat gaaacgcatc tgcaacagca
     4801 aactcaatat ctaatccact gttttttgcc ttctcccggg caatagcgat tgcagtttca
     4861 gctacatcca tccccacgac ctgcaatcca agcgaggcga tgagaagagt attctcgcca
     4921 gtcccgcagc ccacatcgag caccggtccc ataaatccac cctcggatgc taatcgcgca
     4981 atcgctggct gtggctggcc gatgtcccat ggggcagcgc cattcaggta cgacgcatcc
     5041 cagggcagtc ccgtcatttt ttcgtggctc gtggggggcg ccggccatgg aatggatcgt
     5101 ccacgaagtt tcctgattgc ttcggcatga tggctcctct tcatccattt tcaaacttat
     5161 aatctgttct ttgcgctgcc agcaaaagat gataaccact ggaaaaggac atactgggaa
     5221 attgcagata gccaataaga aatattgtgc atgctctgct tccaaagaag acaaatacaa
     5281 gatacatgct gaatgttggg acgggcatat aggaaagaaa attccggaca cgcattacgc
     5341 atatccaaaa gaagagcatg gcccacaagc gctgctatca ctattcataa cgaatggcct
     5401 tgactgggtc gagagcggct gctttgtgtg ccggaagcca gcaggcaaac agacacacaa
     5461 cagcgagcag caacgccaca attgcaaaca gcaaaggatc agatggagta atgccaatcg
     5521 gcaaacttcc aatcagccgt gctgccggca cggccgcagc cataccgagc gtgagtcctg
     5581 ccgtgatctg ccacaggccg cgcctcatga ccagcatcag gatgttgcgc gcgtttgcgc
     5641 ccagggccat gcggacacca atctcctgcg tgcgggcatt ggtggcgtga gcaataacgg
     5701 cataaatgcc aacagaagcc atcagcagcg ctatcaatgc gaatccggcg aagagcttgc
     5761 taaacaagtg gagataccaa agctggtgat cgagtgcctt cgggaatgta tagacttctc
     5821 gcagaggcag atcctgatcc aaaccctgca cagcagcgcg gaccgcattg attggattgg
     5881 tattcgaccg gaccatcagc gccatactgc tccagccctt ttcacggtag ggcaggaaaa
     5941 gcagcggctt cggatgtaca tcatcgagct gctggacaag gttggcagag actccgacga
     6001 ttgtgagcca gtcgccgggg ttgttttttt catcgtagaa gcggaaccgc tttccaatag
     6061 cgttctggtt tggccagaag tgttgggcga actcgcgtgt gacgatggct gctttgtggt
     6121 tcgcagttcc gtcggtctca ttgaagtccc gcccttgcag aagaggaagg ttgatcgtgg
     6181 caaaataacc gggcccctgc acaagataac tgacagcagg gcggtgcgcc gggttgtcta
     6241 tcgtggagtg ctcaatctca atctgagatg tgttggcccc aagacctggt aaatctgaga
     6301 cgagggctgc gtggcttacg ccgggaattg ccttgaggcg cggcagcagc tggttgtaaa
     6361 aacgctggcg cgcatcggta tctttgtaat ggccgtcagg aagatcgagc cgcgccgtca
     6421 tcagttgatc cgcaggcaca tcgcggtttg cagagaatgc cttcagcaga ctctgcacaa
     6481 agaatcctgc gccggtaagc agcacaagag tcagggcaag ctgaaaaatt acaagcaggc
     6541 cggaaagtct gccgccgcga tgtttaccag cactcctggc gccttctttc agcacttcgt
     6601 tcaaatccac ccgtgaggag cgcagggccg gggccatccc aaagagcaga ccgcagaaga
     6661 tgcagagcac ggcaaaatag ccgaaaacag catagtccat tgtgaactcg acccaatagg
     6721 gtttgccgac attctgcgtg gagagatcaa accagtgcac tccaaaagcc gccagtgcaa
     6781 ggccgagtat gccgccaaag atgctgagca gcacactttc catcagcagt tggcggacaa
     6841 tgagccagcg cgatgctccc agagcggcgc ggatagacat ttcgcgctgc cggaccagag
     6901 cgcggcccag catcatattc gcaacattgg cgcatgcgat gagcaacaca aatcctacgg
     6961 cggccaacat aagaaggaag accatgcgga tgtttccgcc gttgtagtgt tgctgaaatg
     7021 tctctacgga agtctcaatg tctttatttg tgacgggata ttgttttgcc agccggtctg
     7081 caatttgatt cagctccgcc tgggcctcgg taacagttgt tcctggcttg agtataccga
     7141 agagctggag cggacggttg tcacgttttt ccagctctgg cgttggcgtc agaggcatcc
     7201 agatatcggt attggtaagg aacttaaatc cttttggcat gacgccgaca atggtggccg
     7261 gtcttccatt cacccgtacc tgacggccga ttacgctggg cgaactgttg taccgttctt
     7321 tccagactcc atatcccagg accaatacag gttctgctcc cggcttgtcg tccgttggaa
     7381 ggaacgcacg gcccagaatt ggatggatgt tcagacttga aaaaatgccc gatgaggcac
     7441 gctccagcga ataagcctga ggaggattgc tattttcact aagcacgccc tcgtcattga
     7501 acgctgcatt cagtgattca aacgacgtag cctggacgcg ataatcgagg aaatcgggat
     7561 acgacacccg actcctgtcc ggaccacgcg taacgttccg actgttgatg gccaccagcc
     7621 gttctccgcc ggcgacgggc acagatttca tcagtgctgc attcacgagc gtaaaaatca
     7681 tcgtgttcag acctatgccc aatgccaatg tcgccactat cgcggctgaa aaccagcgat
     7741 gagagaagat catgcgcaaa gagagccgca ggtcacgtgt catgcaatcc ggatacgcag
     7801 aggagcactg gccagttcca cagaatgcag aaatttattc agcagattac aaggtgcgct
     7861 taaacagagg agttgcgatg aggagcgcca ccacgccgaa tcccaataaa aagagcaagt
     7921 cagaccaaat ggcggcaaat ccgccgtctt tcagcaaaat ggccttgaat ccgtggaccg
     7981 cgtaggtgaa cgggtctacc acggcgatgg cacggagcca cttgggaaag cctgaaatgg
     8041 gggagatggc cccgctgggg aaaaacagca gcgtattcag cacgccgaac atagcgcgag
     8101 gaaccagagg atcttctacg cgcaccatca gcagaaacat catgccattg aaggcgatag
     8161 atgtagtgag gataagaagc gagagttgca gaagcgtaat gggatggaag atgactccca
     8221 gccctgcgag cagcgtgccg atcacagtaa ggcttacgcc ggagattacg gccttcaaag
     8281 cgccggcgag attcaggcca agcaccaact caagc
//
LOCUS       DASRBV010000266        13922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_223743, whole genome shotgun sequence.
ACCESSION   DASRBV010000266 DASRBV010000000
VERSION     DASRBV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13922
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223743"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFA02_09760"
     CDS             <1..1194
                     /locus_tag="VFA02_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HZU10180.1"
                     /translation="FVWGFLTSLNDILIPHLKSIFSLNYAEAMLVQFAFFCSYAAFGI
                     PSGMVVEWIGYQRTMVAGLFTMCVGALLFIPAASVPSFALFLTALIILAAGITALQVA
                     ANPYVSVLGPPETASSRLNLTQAFNSLGTVIGPLLGGWLILRGAKVLDTRTLSPQALH
                     AYRIHEAATVKFPYFAIALALIVLGLAIALYKFPRIDTTKDYRPMGEKKDSIWRHRHV
                     VLGAVAIFVYVGAEVSIGSFLINYFNQPDIGGLTALAASALVPFYWGGAMVGRFIGSA
                     ILQKIQTGKLLGLCAIVATVLVLTSMLTFGHVAIWSILLVGLFNSIMFPSIFTLGIAE
                     MGPLTGEASGLLVTAIVGGALIPELQGMLADRIGIHHAFVLPVLCYLFIAYYGFRGSK
                     VVLPE"
     gene            complement(1198..2049)
                     /locus_tag="VFA02_09765"
     CDS             complement(1198..2049)
                     /locus_tag="VFA02_09765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU10181.1"
                     /translation="MPRYEYRDLRPSREAEEVFLRWINYLDEEFTKHRKPELRAELVY
                     DNLHQLFLGRPRGGRLNFTLTTELPFNVLQLSLDSANITLEPEYYGDIDEKKYACIKP
                     LIYFWQMFDRSPVGLNHWLGFRFRAMLGKHIFKHIGKNVKIFHGVEFSYGYNLTIEDD
                     CVIHKYVMLDDRGELILRKGTSISDYAAVYSHAHDPRDPMIVENRKTEVGPYARVTYH
                     AAVMAGVNIGEDSMLGAMGVATRDVAAHSIVGGVPAKHIRTKDEAKLFADRETYKRPP
                     VTGEHCE"
     gene            complement(2170..2898)
                     /locus_tag="VFA02_09770"
     CDS             complement(2170..2898)
                     /locus_tag="VFA02_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZU10182.1"
                     /translation="MKKWAAFGALVFAVAAVANAQTIKVDKDNRTIAVTASDKAVTDA
                     DLAVVHIGFQVFGPDEQTTYANGSKVSNAIMDALKKTGVPEKSIESENQSIAPNNYHD
                     PKESEMERARKQFVLNQSWTVKAKPDDAAKVLHAAVDAGANQSGQIDWQYQDMNGLQA
                     KAAANALKKAQAVAQQMADGLNVKLAGLIYASNQAPESPVRPMPMVAMKAQAFGAAER
                     VAPLAINPRQIEESATVYAVFAIQ"
     gene            complement(3029..4108)
                     /gene="trpD"
                     /locus_tag="VFA02_09775"
     CDS             complement(3029..4108)
                     /gene="trpD"
                     /locus_tag="VFA02_09775"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZU10183.1"
                     /translation="MLKELNGEQPGEQLREQRGEQRSLTREQARAVLREMLTGEVADS
                     EIAALLTAMAQRGETVEELTGFAEAMLELSVQVPLTEEERALLIDTCGTGGDDRGTFN
                     ISTGAALVAAAAGAKVAKHGNRAVTSKCGSADVLEALGVAVALSPELAVESLRTAGFM
                     FLYAPALHPAMKRVAPIRRALGFRTIFNLAGPLTNPAGAPAQVMGVFAANRVSVVAET
                     MLQLGVRHAFVVHGSDGLDELTITGPSHVAEVQCTGLRRQVSGSMRLFEIAPEEAGLQ
                     RAPISTLAAAESAARNAEILERIFAGEQGPKRDIVLLNAAAALVAGRIATSLRQGVER
                     AAEAIDSGAVLATLKKLREFAKKIG"
     gene            4199..4669
                     /gene="msrA"
                     /locus_tag="VFA02_09780"
     CDS             4199..4669
                     /gene="msrA"
                     /locus_tag="VFA02_09780"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZU10184.1"
                     /translation="MEKATFGAGCFWGVEASFAQIPGVVETAVGYEGGTMDQPTYKDV
                     CTDQTGHAEVVELTFDPARISYEQLLEAFFSLHDPTQLNRQGPDWGTQYRSVIFFHSP
                     GQEAAAKRMIERLTAEKRFSKPIVTQVVPAKTFWRAEEYHQKYLEKRGLASCHI"
     gene            complement(4826..6967)
                     /locus_tag="VFA02_09785"
     CDS             complement(4826..6967)
                     /locus_tag="VFA02_09785"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HZU10185.1"
                     /translation="MIDKFVRVPNYIALILVASSCFLCARAQKPLVIRPGVTLVVGAD
                     EPGPVQKAAEDLAGDFEKVLGKKPAIVQREQDAGASAIVIGGVSRPSGLTAPESFSIS
                     ADRVKRGGNFHEVVLLTGADMRGTIYAIYEFSQEFLGVDPLYYWTDQQPAHRDSIMIP
                     ANLHKVFSAPVFRYRGFFINDEDLLTGWAPGFRDHTGISLAVWDKIYETILRLKGNMV
                     APGTWIFPDDAQVKLASERGLVITQHHAIPLGLNVARWPADVPYNYSTHPEILERAWK
                     NAVSEYGPNQEVLWTVGLRGLSDVTYASMDPNVRGNNQALGKLITKAIADQMSIVRRV
                     HPDAKFVTSLWQEGARLVQQGDLKIPPEVMTVWADDGYGYLQDNGEVAAGQGAYDHIA
                     MMNGRANQLTEMVPVERSFSEIARYIKAGATQYYLVNTSDIRPVTMSARAVMDAVWKG
                     VPDSADTFYQQWAKEEFGEKAASAIAEIYKEYFKAPAHFGNPVHEYGDQLYHTEARRM
                     LLTYMIDSPLYALPSQAPKWEPPRIVGEGLGRPSAPVARKEWLKTAVEREIQQCGEAQ
                     PRWDDVWNKAVAAEALVGPSRLPFYREQVLAMIAISRESNRILYLVSKAIQDADNGRT
                     EQAHSKAVEALKAFDAIQKAEDDAEYGKWANWYRGDWLTGIPRTRELTQIFANYLEDP
                     MTPLAPPVLWDGWEAYYHIMQYEGERSADVQ"
     gene            complement(7030..7620)
                     /locus_tag="VFA02_09790"
     CDS             complement(7030..7620)
                     /locus_tag="VFA02_09790"
                     /inference="COORDINATES: protein motif:HMM:NF007797.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HZU10186.1"
                     /translation="MQPRVQDLGRFTLPPNFRGRPAWFVQLWWIVQSTLFRCSPQFMY
                     GWRNFLLRLFGARIGKGVIIRPTAEITYPWKLTIGDYVWVGDHATLYTLGEIYIGDNA
                     CVSQYSYVCAAAHDYLKPTFDIYSRAVVIEPEVWLATNVFVAPGVTVGRGAVVGACSV
                     VTKDVPPMVICAGNPAKVIRPRPVGEEAAAVLENRA"
     gene            7684..8583
                     /gene="dapA"
                     /locus_tag="VFA02_09795"
     CDS             7684..8583
                     /gene="dapA"
                     /locus_tag="VFA02_09795"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZU10187.1"
                     /translation="MDLRGCGTALVTPFRADGSIDEPALRSLAQWQIESGIDWLVACG
                     TTAETPTLDEEEWLRVTRIVADVASGRVPVWAGCTHNSTRETVRKAQAAAQVPGVSAI
                     LVANPYYNKPGQEGQYQHFKAVARAVHLPVVLYNIPGRTGTNLEPATVLRLLQDAPNI
                     AGVKESSGNLPQITELITQAPQGFHVYAGDDNMTLGIIATGGAGLVSVASHEIPAEMA
                     QMVHAALAGDWETARQINRRYYRLMQANFWETNPAPVKAMLAMMGRIEEHCRLPMVPV
                     SSATRARLEKVAGEVGLLVKTPQ"
     gene            8584..9204
                     /locus_tag="VFA02_09800"
     CDS             8584..9204
                     /locus_tag="VFA02_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10188.1"
                     /translation="MRRLSNTFSMPLFLLLYAMTAHGAHGPSSCAAKIHSFKDSAPLA
                     TLTKQQQKKVLQALSDDIAEIAEVDIASTEKSKAAYRQYVAQRLQFQPVRPASSKEQL
                     LLISYHSNTVCGQHENCPVWIVSLDASKARSLVPWQKELGTSAGGGWGFVSRPDGAAP
                     YSELILLTHLSSAQTGLACYRETKKQYLRVPCSPECSRWLQYADEW"
     gene            9233..10078
                     /locus_tag="VFA02_09805"
     CDS             9233..10078
                     /locus_tag="VFA02_09805"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZU10189.1"
                     /translation="MQTTHLQKKIEEYFAAGQSAVGDPAAMAAFEELRDALEAGTIRS
                     AEPDASAPAGWRVNAWVKQGILLGFRLGQMTASGSDLSFVDKHTYPPRKFTPEQGVRI
                     VPGGSSVRSGAYLAKSVVCMPPMYVNVGAYVDEGTMVDSHALVGSCAQIGKRVHLSAA
                     AQIGGVLEPVNAGPVILEDDVLVGGNCGVYEGTIVRKRAVLAAGTVLTRGTPVYDVVN
                     GTVLRASGDLPLIIPEEAVVVPGARAISRAKGQEWGLSVYTPVIVKYRDEKTELSLAL
                     EDLLR"
     gene            10104..10601
                     /locus_tag="VFA02_09810"
     CDS             10104..10601
                     /locus_tag="VFA02_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU10190.1"
                     /translation="MQRTTGDNLLEVILDSWDRNNTILINLLHALPEGGLDARAIEDS
                     PSVVEMLTHIHYVRLVFVSEDAPEFASALPQQEWRHERDRSRIAQMLNESAKVVRDAV
                     KGRLEAGREMELHYDHPLLMLQHMIWHEGYHHGQIKLALKHMGRPLEDEAAGPVTWDI
                     WMDKK"
     gene            10829..11521
                     /locus_tag="VFA02_09815"
     CDS             10829..11521
                     /locus_tag="VFA02_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HZU10191.1"
                     /translation="MSTTAVSPRALRVVGFWDSTNGKKVVMAITGAIMFLFVIGHLAG
                     NLQIYEGRDKFNGYAHFLRTIGEVLWTVRVILIASVILHITATIQLAHRKKAARPVGY
                     SRKKAIASSYASRTMYWSGPIILAFVIFHLLQFTAGYIHPESRFIEGDVYHNVIAGFS
                     VWWVSAWYIFSMVLLGLHLRHGIWSMFQSVGFNHPRHTPILKSAALVIATLIVLGYIS
                     IPISVLLGFVKE"
     gene            11518..13431
                     /locus_tag="VFA02_09820"
     CDS             11518..13431
                     /locus_tag="VFA02_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZU10192.1"
                     /translation="MTLDGKVPSGPIEQAWDKTRFEMKLVNPANKRKHTVIVVGSGLA
                     GASAAATLGELGYNVRCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVK
                     GGDFRAREANVYRLAQISVNIIDQCVAQGVPFAREYGGTLTNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALERQVNAGTVKMFPRTEMLDLIVIDGKARGIVARNLVTGAIDIHM
                     ADAVILASGGYGNVYYLSTNAKGSNVTASWRAHKRGALFANPCYTQIHPTCIPVSGDY
                     QSKLTLMSESLRNDGRIWVPKQKGDKRPPNQIPEDERDYYLERKYPSFGNLVPRDVAS
                     RNAKYVCDEGRGVGETGLGVYLDFSDAIKRLGEQKIRERYGNLFDMYQRITDEDPYKV
                     PMRIYPAIHYTMGGLWVDYYLQSTIPGLFVLGEANFSDHGANRLGASALMQGLSDGYF
                     IIPYTLGNYLASTKLEKVSESHPEARNAEACVKMTIEKLLSIKGKRTVDSFHRELGKI
                     MWDYCGMSRTAEGLEHAIQKIRELREEYWQNVNVPGSGDDLNQSLEKAGRVADFFELG
                     ELMCIDARDRNESCGGHFREEYQTPDGEAQRDDEHYSYVAAWEYRGPGNQPELHKEPL
                     EFHYVHPSQRSYK"
     gene            13432..>13922
                     /locus_tag="VFA02_09825"
     CDS             13432..>13922
                     /locus_tag="VFA02_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZU10193.1"
                     /translation="MKLTLKIWRQKNPNQAGKFVSYTVDNVNPDMSMLECLDVLNETL
                     IERGEEPVAFEHDCREGICGSCGFMINGIPHGPLPATTVCQLTMRHFKDGQELTIEPW
                     RAASFPLIKDLVVDRRAFDRIIASGGYISINTGNAPDANCIPVGKDIADKAMDAAACI
                     GCGA"
BASE COUNT         3019 a         4292 c         3652 g         2959 t
ORIGIN      
        1 tttgtgtggg gctttctcac ctcgctgaat gacattctga ttccacacct caagtccatt
       61 ttttccctga attatgccga agccatgctg gtgcagtttg ccttcttctg ctcctatgcg
      121 gcctttggaa ttccgtcagg catggtcgtt gagtggatcg gctatcagcg caccatggtc
      181 gccggactgt tcaccatgtg cgtcggagcg ctgctgttta tcccggcagc cagcgtgcct
      241 tcctttgcct tatttcttac cgccctgatt attctggccg ccggaatcac ggccctccag
      301 gtagcagcca acccatatgt ttcagtgctg ggacctcccg agaccgcatc cagccgcctg
      361 aatcttaccc aggcattcaa ttctctgggt acggtcattg gcccactgct cggcggctgg
      421 ctgattctgc gtggcgcaaa ggtcctggac actcgcacgt tatctcccca ggccttgcat
      481 gcctatcgca tacatgaagc ggcaaccgtt aaattcccct actttgcaat tgctctcgcc
      541 ctgatcgtgc tgggccttgc cattgcgctg tacaagtttc cgcgtattga caccaccaaa
      601 gactaccgcc ctatgggcga aaagaaagac agcatctggc gtcatcgcca tgtggtgctg
      661 ggagccgtgg caattttcgt ttacgtcgga gcagaagtct ccatcggcag ctttctgatt
      721 aactacttca atcagcctga cattggcggc ctgaccgcgc tcgcggcatc tgcgctcgtc
      781 cccttctatt ggggaggcgc aatggtgggc cgcttcatcg gctcagcaat cctgcagaaa
      841 atccagaccg gaaaattgct gggcctgtgc gccattgtgg ccactgtact cgtgctcacc
      901 tctatgctta cctttgggca tgtggccatc tggagcattc tgctggtcgg cctcttcaac
      961 tccatcatgt tccccagtat cttcacgctc ggcattgcgg agatggggcc gctcactggc
     1021 gaggcctccg gcctgctggt cactgcgatc gtcggcggcg cgctcattcc tgaattgcaa
     1081 ggcatgctcg ccgaccgcat cggcattcac catgcgtttg ttctgcccgt gctctgctat
     1141 ctcttcatcg cgtattacgg attcagagga tcgaaggtcg tccttccgga ataggcctta
     1201 ttcgcaatgt tctccggtta cgggaggccg cttataggtc tcccggtcgg cgaacagctt
     1261 tgcctcgtcc tttgtccgga tgtgcttcgc cggaactcct cccacaatgc tatgtgcggc
     1321 aacatcacgt gtggccacgc ccattgcgcc cagcatcgag tcttcgccga tattgactcc
     1381 agccatcacc gcggcgtgat aagtgacgcg cgcatagggg ccgacttcgg tcttccggtt
     1441 ctctacaatc atgggatctc tcggatcatg cgcgtgtgaa tataccgccg cgtagtctga
     1501 aatcgaagtc cccttccgga gaatcaattc gccgcgatcg tcgagcatca cgtatttgtg
     1561 gataacacaa tcatcttcaa tcgtgagatt gtagccataa ctgaactcga ccccgtgaaa
     1621 gatcttcacg ttctttccaa tatgcttaaa gatgtgcttg cccagcatgg cgcgaaagcg
     1681 aaagcccagc cagtggttca ggccaacggg cgaacggtcg aacatttgcc agaaatagat
     1741 caacggcttg atgcaggcat atttcttttc atcaatgtcg ccgtaatact ccggttccag
     1801 agtaatgttg gcggaatcca gcgaaagctg gagcacattg aaaggaagct ccgtcgtcaa
     1861 cgtaaagttc agccgtcccc ctcggggcct gccaagaaac aactggtgca gattgtcata
     1921 caccagttcg gcgcgaagct caggtttgcg gtgcttcgtg aactcctcat caagatagtt
     1981 gatccagcga agaaagacct cctctgcctc acgcgaaggc cgaagatcac gatattcata
     2041 ccgaggcata ggttgtcatc ctgagcagat ctaaaaattt tgccgtcccg agcgcagcac
     2101 agtcgaagga cctgcttttc tctccatggc actacgattc agatgcgcca catgtcccaa
     2161 cgtcgcacct cactggatcg cgaacactgc atacaccgtt gccgattctt caatctggcg
     2221 tgggttgatg gccagtggag caactctttc cgcagctcca aatgcctgcg ctttcatcgc
     2281 aaccattggc atcggacgca caggactctc aggggcctga ttactggcat aaatcagtcc
     2341 ggccagcttc acattcaatc catcggccat ctgctgcgca accgcctgcg cctttttaag
     2401 agcattggcc gcggcctttg cctgaagacc attcatgtcc tgatactgcc aatcaatctg
     2461 accgctctgg ttggctcctg catccactgc cgcatgtagc accttcgcag catcgtcggg
     2521 cttcgccttc accgtccatg actggttcag aacaaattgt tttctggcgc gctccatctc
     2581 cgattcctta ggatcgtgat aattgttcgg cgcgatgctc tggttctcgc tctcaatgga
     2641 cttctccggc acacccgtct tcttcagagc atccatgata gcgttagaaa ctttggaacc
     2701 attcgcgtac gtggtctgct catccggccc aaagacctga aagccgatgt gtaccacggc
     2761 cagatcagca tccgtgaccg ccttatcgct ggccgttacc gcgatagtac ggttgtcctt
     2821 gtcaaccttg atggtctgcg cgttcgcaac cgctgccact gcgaaaacca atgcgccgaa
     2881 agctgcccat ttcttcatgc gtgccgattc ctcctgtgca tcacaacagc actcgcaagt
     2941 atagtctttt caccacaaag ggcacagaga gcacagcgga cccagggaaa aactcttgga
     3001 ctctatgtgc tctgaagtga aagatgtttt acccgatctt cttcgcaaac tcccgcagct
     3061 tcttcagggt tgccaacacg gccccggaat caatcgcctc agccgcgcgc tctacgcctt
     3121 gacgaagact ggtcgcgatt ctccccgcca ccagagcagc tgcggcattc agcagcacaa
     3181 tgtcgcgctt cggcccctgc tcgcctgcaa agatgcgctc cagaatctca gcattgcgcg
     3241 ctgcactctc cgcggccgcg agtgtagaaa tcggagcacg ctgcaacccc gcttcttccg
     3301 gcgcaatctc gaagagccgc atactgcctg agacctgtct cctcaggcct gtgcactgga
     3361 cttccgccac atggctcggc ccggtaatcg tcaactcatc cagtccatcg cttccatgca
     3421 caacgaaggc atggcgcacg cccagctgca acatcgtttc cgcaaccacg ctgacgcggt
     3481 tggccgcaaa cacgcccatt acctgtgccg gagcgcctgc cgggttcgtc agcggccccg
     3541 ccaggttgaa gatggtgcga aatcccagtg cccgccgaat cggcgctacc cgcttcatcg
     3601 ccggatgcag cgccggagca tacagaaaca taaatcccgc tgtgcgcaac gactccaccg
     3661 caagctccgg agacagcgcc accgccacgc ccagcgcctc cagcacgtct gccgaaccgc
     3721 acttcgaggt cacggcgcga ttgccatgct ttgccacttt cgcgccggca gccgccgcaa
     3781 caagcgccgc tccggttgag atattgaacg tgccgcggtc atctccgccc gtaccgcagg
     3841 tgtctatcaa cagcgcgcgc tcttcttccg tcaacgggac ctgaacggaa agctccagca
     3901 tcgcctcggc aaagccagtg agttcctcta cagtctcgcc gcgctgtgcc atggcagtga
     3961 gtaacgcagc gatttcagag tcagccacct ctccggtcag catctcacgc agaacagcac
     4021 gggcctgctc gcgtgtcagg ctgcgttgct ctccgcgctg ctcccggagt tgttccccgg
     4081 gttgctcccc gttcagttct ttcagcatgg gcatctcaat ttatcgtacc caaggacgtg
     4141 gccggctgct atgaatcgcc cggcagaccg gcgtaatctt aaagaaaaga ggtgttctat
     4201 ggaaaaggca actttcggcg caggatgctt ctggggcgtg gaagcaagct ttgcgcaaat
     4261 ccccggagta gtagaaaccg ccgtcggcta tgaaggcgga acgatggacc aaccgacata
     4321 caaagacgtc tgcaccgacc agaccggaca cgccgaagtt gtagaactga ccttcgatcc
     4381 cgccaggatc agctatgagc agttgcttga ggcattcttc tccctgcacg atcccaccca
     4441 actcaatcgc caggggccgg actggggcac acagtaccgc tccgtcatct tcttccattc
     4501 ccccgggcag gaagccgccg caaaacgcat gatcgaaagg ctcaccgcag aaaagcgctt
     4561 ctcaaaaccg atcgtgaccc aggtcgtccc ggcaaagacc ttctggcgtg ccgaagaata
     4621 ccaccagaag tatctggaaa aacgcgggct ggcaagctgc catatctgaa acttatagcg
     4681 ccggaatttg ccgcattcac atatggaaaa actcgcgcgt tttcatctac tttgctggat
     4741 ccgcgggtcc ataagaactg aacaatacaa ccgtgaacaa gcacacatgg cgcccgaagt
     4801 cgtgtatcgc tggacgcgaa gtccctcact gcacatctgc cgacctctcc ccttcatact
     4861 gcatgatgtg gtaataggcc tcccatccat cccatagcac tggaggggcc aacggcgtca
     4921 tcgggtcttc cagataattc gcaaatatct gcgtcagctc gcgggtgcgc ggaatacccg
     4981 taagccagtc tccgcgatac cagttggccc acttgccgta ctcggcatcg tcctcggctt
     5041 tctgaatggc atcgaatgcc ttcagcgcct ccactgcctt gctgtgcgcc tgttcggtgc
     5101 ggccattgtc cgcgtcctga atggctttcg aaacaaggta cagtatccga ttgctctcgc
     5161 ggctgatggc gatcatcgcc agcacctgct cccggtaaaa cggcaaccga gacggcccaa
     5221 cgagcgcttc agctgccacg gccttgttcc acacatcgtc ccagcgcggc tgcgcctctc
     5281 cgcattgctg gatttcgcgc tcgacagccg tcttcagcca ctcctttctg gcgacgggcg
     5341 cagaaggccg tccgagccct tcaccaacaa tccgcggagg ctcccatttc ggggcctggc
     5401 tcggcagcgc atacagcggc gaatcaatca tgtaggtcag cagcatccgc cgggcttccg
     5461 tgtggtaaag ctggtcacca tactcatgca ccggattgcc gaaatgcgcc ggggccttga
     5521 agtactcttt gtagatttca gcaatcgctg aagcggcctt ctcgccgaac tcctccttcg
     5581 cccattgctg atagaacgtg tcagcgctgt ccggaacacc cttccacacc gcatccatca
     5641 ccgcgcgcgc cgacatcgtc accggacgaa tgtcgctcgt gttcaccaga taatactgcg
     5701 tggccccggc tttgatgtac cgcgcaattt cagaaaagct gcgctccacc ggcaccatct
     5761 ccgtaagctg attggcccgg ccattcatca tggcaatgtg gtcatacgcg ccctgccccg
     5821 cggcgacttc accgttgtcc tgcaggtacc cgtagccatc atctgcccat accgtcatca
     5881 cctcaggagg aatcttcaaa tcgccctgct gcaccagccg cgcaccctcc tgccacaggc
     5941 tggtcacaaa tttcgcatcc ggatgtaccc tgcgaacgat gctcatctgg tcggcaatgg
     6001 ctttggtgat aagcttcccc aacgcctgat tgttaccacg cacgttcggg tccattgaag
     6061 cataggtcac atcagataga ccgcgcagac ccaccgtcca cagcacttcc tggttcggcc
     6121 catactcgct cacagcattc ttccacgccc gctccagaat ctccggatgc gtcgaatagt
     6181 tgtacggaac atccgcaggc cagcgcgcca cattcagtcc cagaggaatc gcgtgatgct
     6241 gcgtgatgac aagcccgcgt tccgatgcta gcttcacctg cgcatcatcc ggaaaaatcc
     6301 acgtccccgg cgcgaccatg ttccctttca gccgcagaat ggtttcgtaa atcttgtccc
     6361 acaccgccag agaaattcct gtgtggtctc tgaatcccgg cgcccagccc gtcagcaggt
     6421 cttcatcatt gataaagaac ccgcgatacc ggaagacagg cgcagaaaag accttgtgca
     6481 gattggcggg aatcataatc gaatcgcgat gcgcaggctg ctggtcagtc cagtaataca
     6541 gcggatccac gccgagaaat tcttgcgaaa actcgtagat ggcatagata gtgccgcgca
     6601 tgtctgctcc ggtcagcagt acaacttcgt gaaagttgcc gccgcgcttc accctgtctg
     6661 ccgaaatgga aaatgactct ggggcagtca gtccgctagg tctggatacc cctccaatca
     6721 caatcgcgct tgctcccgcg tcctgctcgc gctggacaat cgcaggcttt ttgccgagca
     6781 ctttctcaaa atcacccgca aggtcctcag ccgccttctg tactggcccc ggttcatcag
     6841 cacctacaac cagagtcacg cccggccgga tcaccagcgg cttctgcgct ctggcgcaaa
     6901 ggaaacagga actcgccact aaaatcagag caatatagtt cgggacacgt acaaatttgt
     6961 caatcaattg tcttcaccct cctgctcatc gctgaaaata tctggaactg atcgcggaat
     7021 gattttagct caggcccggt tctccaaaac agccgctgct tcttccccta cgggacgcgg
     7081 cctgatcacc tttgcgggat ttccggcgca gatcaccatc ggaggaacat ctttggtgac
     7141 aacgctgcag gctcccacaa cagcgccgcg gcccaccgtc acgcccggag caacaaaaac
     7201 attcgtcgcc agccatactt cgggttcaat cactaccgcg cgcgaataaa tatcaaaggt
     7261 aggcttcaga taatcgtgtg cggccgcgca aacataggaa tactgcgaaa cacacgcatt
     7321 gtcgccaata tagatttcgc ccagtgtgta cagcgtggca tggtcgccga cccatacgta
     7381 gtcaccaatg gtcagcttcc agggatacgt aatctccgcc gtaggccgga tgatcacgcc
     7441 cttgccgatc ctcgccccga agagacgaag cagaaagttt cgccagccat acatgaactg
     7501 tggcgagcag cgaaagagcg tgctctgcac gatccaccag agctgcacaa accacgctgg
     7561 ccgtccgcga aaattcggcg gcagggtaaa tcttcccagg tcttgtacgc gaggctgcat
     7621 cgtgcattca gtttagtgca agttgtcaac cgtcgcattc aacgcagtgt aaactcatca
     7681 ttgatggact tgagaggttg cggcactgcg ctggtcactc cgtttcgcgc tgacggttcc
     7741 atagatgaac cggcactgcg ctcactggcg caatggcaga tcgaaagcgg aatagactgg
     7801 ctggtcgcct gtgggacgac cgcggaaact cccacactcg atgaggagga gtggctgcgc
     7861 gtcacccgca ttgtggccga tgtcgcctct ggcagggtac ccgtctgggc aggatgcacg
     7921 cacaactcca cacgcgaaac tgtacgcaag gcccaggcgg ccgcccaggt ccccggcgta
     7981 tctgcaattc tggttgcaaa tccgtactac aacaagccag gacaggaagg ccaataccag
     8041 cacttcaagg ccgttgcccg tgctgtgcat ctgcccgtag tgctttacaa cattcccgga
     8101 cgaacaggca cgaaccttga accggcaacc gtgctgcgcc tgctccagga cgctcccaat
     8161 attgccggcg taaaggaatc gagcggcaat ctgcctcaga tcactgagct gattacgcag
     8221 gcaccgcaag ggttccatgt ctatgcaggt gatgacaaca tgacattggg catcatcgca
     8281 acaggtggcg cgggactcgt ttccgtcgcg tcgcacgaaa ttccggcaga gatggcgcag
     8341 atggtccatg ctgctcttgc cggcgattgg gagactgcgc ggcagatcaa ccgcaggtat
     8401 taccgcctca tgcaggccaa tttctgggaa acgaatcctg ctccggtaaa ggccatgctg
     8461 gccatgatgg gccgcattga agaacactgc cgtttgccca tggtccctgt atcgtctgca
     8521 acgcgtgcaa ggctcgaaaa agtggcaggc gaagtgggac tgctcgtgaa aacaccgcaa
     8581 taaatgcgcc gtctttccaa tacgttttcc atgccgctgt ttctgcttct ttatgcaatg
     8641 actgcgcatg gcgcccacgg gccttcatca tgtgctgcaa aaatccattc gttcaaagac
     8701 tccgcgcctc ttgcaacact tacaaaacag cagcagaaaa aagtgcttca ggcattaagc
     8761 gacgacatcg cggagatcgc agaagtagac atcgccagca cggaaaaaag caaggccgca
     8821 tacaggcagt atgttgccca aagacttcag ttccagccag tcaggccggc ctcttcaaaa
     8881 gaacagctct tgctcatcag ctatcactcc aatactgtct gcggccagca tgaaaattgt
     8941 ccggtctgga tcgttagcct ggatgcgtca aaagcgcgtt cgctggtgcc gtggcaaaag
     9001 gagcttggta ccagcgccgg aggcggatgg ggttttgtca gccgtccgga cggcgccgcc
     9061 ccttattctg agttgattct gcttactcac ctctcttccg cccagaccgg actggcctgc
     9121 taccgtgaaa cgaaaaagca atacctgcgc gttccctgct ctcctgagtg ctcgcgctgg
     9181 ctccagtacg ccgacgagtg gtagtccgca gaattgatag actcggtttg tgatgcaaac
     9241 cactcatctc cagaaaaaga ttgaagagta ctttgctgct ggccagtctg ctgtcggcga
     9301 ccccgcggca atggctgcat ttgaagaact gcgtgatgcg cttgaagcag gcacaatccg
     9361 ctctgccgaa cctgatgctt ccgcgccggc cggatggcgt gtaaatgcgt gggtaaagca
     9421 gggcattctg cttggcttcc ggctcggaca gatgaccgct tccggcagcg atctttcatt
     9481 tgtggacaag catacgtatc cgccgcgtaa attcacgccg gaacagggtg ttcgcatcgt
     9541 tcccggcgga tcgtctgtgc gctcaggcgc ctaccttgcg aagtccgtgg tctgcatgcc
     9601 tcctatgtat gtcaacgtag gcgcatatgt agatgaaggc acaatggtgg actctcacgc
     9661 gcttgtagga agctgtgcgc agattgggaa acgcgtccat ctgagcgctg cggcccagat
     9721 tggcggcgtg ctcgagccgg taaacgccgg ccctgtcatc cttgaggatg acgtgctcgt
     9781 aggcggcaac tgtggtgtct acgaaggcac catcgtgcgc aaacgcgccg tgctcgctgc
     9841 cggaacggtc ctgacccgcg gcacccctgt ctacgacgtt gtaaacggca ccgtcctgcg
     9901 agcgtccggc gatcttcccc ttatcatccc tgaagaagca gtcgtcgtcc ccggcgcacg
     9961 cgctatctcc agagcgaaag gacaggagtg gggcctgagc gtttatacgc cggtgattgt
    10021 gaaataccgc gatgaaaaaa cagagctgag cctcgccctg gaagacctgc tgcggtaacg
    10081 atttgaagtg gaaggagcct ggaatgcaaa gaacaaccgg tgacaacctg cttgaggtca
    10141 tactggactc ctgggaccgg aacaacacca tcctcatcaa tctgctccac gccctgccgg
    10201 aaggtggtct ggatgccagg gccatcgagg acagcccctc ggtcgtagag atgctcacgc
    10261 acatccatta tgtgcgcctg gtattcgtct cagaggacgc gccggaattt gcatccgcct
    10321 tgcctcaaca agagtggcgg catgaacgcg atcgcagccg gattgcacag atgctgaacg
    10381 aaagcgccaa agtagtgcgc gatgcggtga agggccgtct ggaagctggc cgggaaatgg
    10441 aactccatta cgaccaccct cttctcatgc ttcagcacat gatctggcac gaaggctatc
    10501 accacgggca gatcaagctg gccctgaagc atatgggcag gccactggag gatgaagccg
    10561 ccggacctgt tacatgggac atatggatgg acaagaagtg atccggaccg gccactgccc
    10621 tcccgcatct ggataaaccg ccatcgctgg aaagaaatct cctccctttt cttgacgatg
    10681 cttctttcgc gcaccaataa taaatagtga cttctccgaa agattcactg gaacgcaagc
    10741 aacactgttc gcgcaacgga cagtacctca actcctgcgg cctgtccttc agcccgcggg
    10801 aaatgaaacc aaaggctggg aagatcgaat gagcactaca gcggtcagtc cgcgcgcgct
    10861 ccgcgtcgtg ggcttctggg actcgacaaa cggcaagaaa gttgtgatgg ccataaccgg
    10921 cgccatcatg ttcctgtttg tgattggcca ccttgccgga aacctccaga tttatgaggg
    10981 acgcgataag ttcaacgggt acgcgcattt tctgcgcacc attggcgaag tgctgtggac
    11041 cgtgcgcgtc atcctgattg cctcggtcat tctgcacatc acagccacca tacagcttgc
    11101 gcatcgcaaa aaagctgcgc gtcctgtcgg atactccaga aaaaaggcca ttgcctcttc
    11161 ttacgcttcg cgcacgatgt actggagcgg cccaatcatt ctggcctttg tcatcttcca
    11221 tctgctgcaa tttactgccg gatatatcca tccggaatcg cggttcatcg aaggcgacgt
    11281 ctatcacaac gtaattgccg gcttcagcgt gtggtgggtc tccgcctggt atatcttctc
    11341 gatggtcttg ctcggcctgc acctgcgtca cggcatctgg agcatgttcc agtccgtggg
    11401 attcaaccat ccgcggcaca cgccgattct gaagtcagcg gcgctggtca tcgccactct
    11461 gattgttctc ggatatattt caattccgat tagcgttttg ctggggttcg taaaggaatg
    11521 acactcgacg gaaaagtacc ttctggccca attgaacagg catgggacaa gacgcgcttc
    11581 gagatgaagc tggtcaaccc ggcaaataag cgcaagcaca cggtgatcgt tgttggttcc
    11641 ggtcttgccg gtgcctcagc cgcggccacg ctgggcgaat taggctataa cgtccgctgc
    11701 ttctgctttc aggactcgcc acgccgcgcg cactcgattg ccgcgcaggg gggcatcaac
    11761 gctgccaaaa actatcagaa tgatggcgac agcgtgtacc gcctcttcta tgacacggtg
    11821 aagggcggag acttccgcgc gcgcgaagcc aacgtctatc ggctggcaca gattagcgtg
    11881 aacattattg accagtgcgt ggcgcagggt gttccgtttg cgcgtgaata cggcggcact
    11941 ttaaccaacc gctcctttgg cggggcgcag gtttcgcgca ccttctacgc ccgcggtcag
    12001 acaggccagc agctccttct cggcgcctat caggcgctgg aacggcaggt aaatgccgga
    12061 acagtgaaga tgtttccgcg tacggagatg ctcgacctca tcgtgattga cggcaaggcg
    12121 cgcggcattg ttgcgcgtaa cctcgtcaca ggcgcgattg acattcacat ggccgatgcg
    12181 gtcatcctcg cttctggagg atacggcaac gtctactacc tttccaccaa cgcgaaaggt
    12241 tcaaatgtaa cggcaagctg gcgcgcacac aaacgcggag cgctgttcgc caacccctgc
    12301 tatacgcaga tccatcccac ctgcattcct gtctccggcg attaccagtc gaagctcacg
    12361 ctgatgtcgg agtcgctgcg caatgacggg cgcatctggg tgccgaagca aaagggcgac
    12421 aagcgcccgc caaaccagat tcccgaggac gagcgcgact attacctgga gcgcaagtat
    12481 ccaagcttcg gcaatcttgt tccccgcgac gtggcttcgc gcaacgcaaa gtatgtctgc
    12541 gatgaaggcc gcggcgttgg cgaaaccgga ctcggcgttt acctcgactt ctcagacgcc
    12601 atcaagcggc tcggcgagca gaagattcgc gagcgttacg gcaatctctt tgatatgtac
    12661 cagcgcatca ccgatgaaga cccatacaag gtcccaatgc gcatctaccc cgccatccac
    12721 tacacgatgg gcggcctgtg ggtggactac tacctgcaaa gcaccatccc cggcctcttt
    12781 gtgttgggcg aagccaactt ctcagaccac ggggccaacc gcctgggcgc aagcgcgttg
    12841 atgcagggcc tttccgacgg ttatttcatc attccctaca cgctgggcaa ttatctggcc
    12901 tccacaaagc tcgaaaaagt aagcgaatct caccctgaag cgcgcaacgc tgaagcgtgc
    12961 gtgaagatga ccatcgagaa gctgctctcc atcaaaggca agcgcacggt ggactccttc
    13021 caccgcgagc tgggcaagat catgtgggac tactgcggca tgtcccgcac cgccgaaggc
    13081 ctcgaacacg ctatccagaa aatccgcgaa ttgcgcgaag aatactggca gaacgtcaac
    13141 gttcccggca gcggtgacga cctgaaccag agcctcgaaa aagcaggccg ggtggccgac
    13201 ttcttcgaac tgggcgagct gatgtgcatt gacgcgcgtg accgcaacga gtcctgtggc
    13261 ggccacttcc gcgaagagta ccagactccg gacggcgaag cacagcgcga tgacgaacac
    13321 tattcctacg ttgcggcatg ggagtatcgc ggccccggca accagcccga gctgcacaag
    13381 gaaccactcg agttccacta cgtccacccc agccagagga gctacaagta aatgaaatta
    13441 acgctgaaaa tctggcgtca gaagaacccc aatcaggcgg gaaaattcgt cagctacacg
    13501 gtagacaatg tcaaccctga catgtccatg ctcgagtgcc tggacgtgct gaatgagacc
    13561 ctcatcgagc gcggcgaaga gccggtggcc ttcgaacatg actgccgtga aggcatctgt
    13621 ggttcctgcg gattcatgat taacggcatt cctcacggcc cactccccgc cacgacggtc
    13681 tgtcagttga ccatgcgcca cttcaaagat ggtcaggaac tcactatcga gccttggcgc
    13741 gctgcttcct tccctttaat taaggacctg gtggtagacc gccgtgcctt cgaccgcatc
    13801 atcgcctcag gcggatacat ctccatcaat acaggcaatg ctccggacgc gaactgcatt
    13861 ccggtcggca aggacatagc cgataaagcc atggatgctg cggcctgcat cggatgcggc
    13921 gc
//
LOCUS       DASRBV010000267         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_422322, whole genome shotgun sequence.
ACCESSION   DASRBV010000267 DASRBV010000000
VERSION     DASRBV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422322"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFA02_09830"
     CDS             complement(<1..710)
                     /locus_tag="VFA02_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU10194.1"
                     /translation="MRVAYFPDSFYEINGVAHTSRHFEAFVRRRNLPFLCVRAGERTP
                     PLLTEGQLTTLELPRGPLSFGLEKDLRFDVGFFRHLPFVARVLRQFRPDVVHITGPSD
                     FGFMGVLLAHHYRLPLVAAWHTNVHEYAARRAEWFLKMLPNGTSPRVAAAIENTSFYL
                     SARFYSIARLLFAPNPQLCQLLEDATGRPCKLMPRGIDTELFSPERRDRTPDDTDFVL
                     GFVGRLSVEKNVALLARIR"
     gene            complement(729..1691)
                     /locus_tag="VFA02_09835"
     CDS             complement(729..1691)
                     /locus_tag="VFA02_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZU10195.1"
                     /translation="MNVFLVHAHPEPQSFSSSMARTAAETLAEMGHSVTMSDLYAMDF
                     DPVSGRKNFTTVKDPEFFKQQQEELHATEHNGFAPEIESEIRKLEAADLLIFSFPLWW
                     FGMPGVMKGWVDRVFPMGRVYGGEKFYENGLGSKRRARAMVLMTTGGGPAGYSGKGVH
                     TPLSSILAPIHHGIFWFNGFLPLDPFIAWSPARITDAERSAYLERLKKRLKDIFAEIP
                     HQPPPLSDFPQFGPDTKKRFQVVVNRKQMPDESFLAKVPQEKARLAELRREGFVLEDA
                     FSPSDAEEWRGFLRVRAASKEEAHEMLKTLPLAGHLDFEIYELA"
     gene            1830..>2051
                     /locus_tag="VFA02_09840"
     CDS             1830..>2051
                     /locus_tag="VFA02_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZU10196.1"
                     /translation="MQIRKFRCQLLAWYDTHARDLPWRRTRDPYAIWVSEIMLQQTRV
                     NAVLDHYARFMDRFPTVQELANATEPEVLA"
BASE COUNT          490 a          586 c          530 g          445 t
ORIGIN      
        1 cgaatccgcg ccaatagcgc tacgttcttt tcgactgaga ggcgccccac aaacccaagc
       61 acaaaatctg tatcgtcagg cgtgcggtcg cggcgctcag gtgaaaacaa ctccgtatca
      121 attccgcggg gcatcagctt acacggacgt ccggtcgcat cctcgagcaa ctggcacaac
      181 tgcggattcg gcgcaaaaag caggcgcgca atcgaataaa accgcgcgct taaatagaaa
      241 ctggtatttt caatcgctgc cgcgacccgc gggcttgtcc cattggggag cattttcaga
      301 aaccactccg cgcgccgcgc cgcatattca tgcacattgg tgtgccatgc ggccaccagc
      361 ggcaggcggt aatggtgcgc cagcagtacg cccataaatc cgaaatcgct tggccctgtg
      421 atgtggacca cgtccggcct gaattgccgc aacacccgcg ctacaaatgg cagatggcga
      481 aagaacccca catcaaagcg aaggtctttt tccagaccaa acgagagcgg gccccgcgga
      541 agttccaaag tcgtaagttg cccttcggtg agcagaggtg gtgtgcgctc ccctgcgcgg
      601 acacacagaa acggcaggtt ccgtcggcga acaaatgcct caaaatgacg gctggtgtgg
      661 gccacgccgt tgatctcgta aaacgagtca ggaaagtacg ccacgcgcat acatagggag
      721 tgtatacgct atgcaagctc gtagatttca aaatctagat gaccggcaag tggcaaggtc
      781 ttcaacattt cgtgtgcttc ttctttggat gcggccctga ctcgcaaaaa gccgcgccac
      841 tcttccgcat ccgaaggcga gaaggcgtct tcaagaacga agccttcccg gcgcaactcc
      901 gccagacgtg ctttctcctg cggtaccttt gccagaaaag actcgtccgg catctgtttc
      961 ctgttcacca ccacttgaaa cctcttcttt gtgtccggcc cgaactgagg aaaatcggac
     1021 aatggcggcg gttgatgggg aatctcagca aatatgtcct tcagccgctt tttcaaccgt
     1081 tccagatagg cagaacgttc ggcgtccgta atacgcgcag gactccaggc aatgaatggg
     1141 tcgagtggga gaaatccgtt aaaccagaag atgccgtggt ggatgggagc aagtatggag
     1201 ctgagcggag tatgaacgcc ctttcctgag tatccggcag gcccgcctcc tgtcgtcatc
     1261 agaaccatcg cccgggcccg gcgcttcgaa cccagcccgt tttcgtaaaa tttttcgcct
     1321 ccgtacacac ggcccatggg aaacacccgg tctacccagc ccttcatcac accgggcatg
     1381 ccgaaccacc acagcggaaa ggagaatatc aaaagatcag ccgcttccag cttgcgtatt
     1441 tcagactcga tctcaggagc aaagccattg tgctccgttg cgtggagctc ctcctgttgc
     1501 tgcttgaaaa actcagggtc tttgacagtc gtgaaattct ttcttcccga aaccggatca
     1561 aagtccatgg catacaaatc agacatggtg acggagtgtc ccatttccgc caatgtctcc
     1621 gccgcagtac gcgccatgga tgaagaaaaa ctctgcggtt ctggatgagc atggacaaga
     1681 aaaacgttca tgaaagaatt atctccattc tgtgcaatgg attggatgtg cgtaaaactt
     1741 tctcgattca aattgaatgc tggctttttc tgattgtttc ctcatgtcct acaatccaga
     1801 atgtgggcgg cgaagcaatc tttgcacctg tgcagatacg gaaatttcgt tgccagcttc
     1861 ttgcctggta tgacacgcat gcccgcgacc tgccttggcg acgcacacgt gatccttatg
     1921 ccatctgggt ctctgaaatc atgctgcaac agacgagggt aaacgctgtt ctcgatcact
     1981 atgcgcgatt tatggaccgc ttccccactg tgcaggagct tgccaatgcg actgaaccgg
     2041 aggtcctcgc c
//
LOCUS       DASRBV010000268         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_224005, whole genome shotgun sequence.
ACCESSION   DASRBV010000268 DASRBV010000000
VERSION     DASRBV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224005"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VFA02_09845"
     CDS             <1..1560
                     /locus_tag="VFA02_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZU10197.1"
                     /translation="ARKAAESIDPEKIRAHVRFLASDLLEGRGPGQRGGQLAAEYIAT
                     QFALDGLKPAGDNGTYFQKVPLYAVHTVEDQTTAEFVPASGQPLSLAYSKDWVAKDQT
                     GQESADIDAPIVFVGYGIDAPEYHWNDYTGLDVKGKVLLVIVNEPPSNDEKFFKGKAL
                     TYYGRWTYKYEEASRLGAAGVLIIHRTDLASYPWQVVRDSQAIEKSYLQGDPAATLKA
                     AAWIQHDVAQKLFTAAGLGNVDDEIARAGQRGFHAVELPIRFKAHIASKVRRYESDNV
                     IAEVPGAQPGQAVIYTAHYDHLGIDPNAKGDNIYNGAADNATGCGILLELARAYSETR
                     PPHPVYFASVTAEEQGLLGSQWLGMHPPVPPSQIALDLNYDMLNPIGIPDSTDVSGAE
                     RTTFYPEVEKTAKDFHLTIEPDQFPGAGHYYRSDHFSLARVGIPAFSIGQGTTFEGHD
                     PEWGKQQMEDFVAHRYHQPSDEYRPDMDFRGDAKMAQFGFILGWEAMSLPKEIGWQPG
                     DEFEAARKKGQ"
     gene            1639..2154
                     /locus_tag="VFA02_09850"
     CDS             1639..2154
                     /locus_tag="VFA02_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10198.1"
                     /translation="MKWRIHILALLFSSASIHAQTANAPKLHHRGDPSTTDHPIVVKP
                     DKGNSLIPDDASGEYMLGKPGEFIEITLQFGDLSGYISRQGDSESDLGTPLTFFFDRT
                     SLQGQQLSFTTRQVHGVWYSFTGTIQRGIGKTRQEDGYYMLTGNLIEHNAAHNTEVRR
                     TVALKSGKQFG"
     gene            2251..2327
                     /locus_tag="VFA02_09855"
     tRNA            2251..2327
                     /locus_tag="VFA02_09855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2285..2287,aa:Pro,seq:ggg)
     gene            2437..3183
                     /locus_tag="VFA02_09860"
     CDS             2437..3183
                     /locus_tag="VFA02_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HZU10199.1"
                     /translation="MKKITLEICLESIDSVIASERGGAQRVELCANLLEGGTTPSAGT
                     LRAARERSSIAINVMVRPRGGDFLYTETEFASMQHDVRMAKDLGADGIVLGLLRADGT
                     VDVDRTRQLVELAAPLPVTFHRAIDVSRDLLEALEAVVSTGAARVLTSGGQPSVADGA
                     PMVAKMVEAAGDRIIVMPGCGITPDNVLSILETTGASEVHIALDEQVQSPMEFRKTEI
                     PMGGMEGREYLRYVTPESAVRKVAQILRRN"
     gene            complement(3281..4576)
                     /gene="fahA"
                     /locus_tag="VFA02_09865"
     CDS             complement(3281..4576)
                     /gene="fahA"
                     /locus_tag="VFA02_09865"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HZU10200.1"
                     /translation="MALMRESWVVSANDGRTDFPLQNLPFGIFSTPGKDPCIGVAIGD
                     SVLNLRACVEQGLFNSLNVRVQKACSEPVLNSLMALGRNTVSAVRQRVMELLAKDAPA
                     GLRNRIEPLLLSIDRVTMHLPAAIGDYTDFYASIYHATNVGRLFRPDNPLLPNYKYVP
                     IGYHGRASSIVISGKDVKRPKGQIKSADMSVPGFGPSRLLDYELEVGFFVSSGNALGQ
                     TIPIGKAEEHIFGLCLLNDWSARDIQAWEYQPLGPFLAKSFATTISPWVITMDALEPF
                     RVPAFRREEQDPDPLPYLNSSDDQTRGGIDITVEVWLSSKLMRDEKTTSVRISKGNLR
                     DLYWTLAQMLTHHTSNGCNLRPGDLLGTGTISGATRDSVGCLLEMTRSGTEPITLPTG
                     ERRKFLEDGDEVVLRGYCEREGHPRIGFGECRGVVIPAD"
BASE COUNT         1102 a         1322 c         1271 g          919 t
ORIGIN      
        1 gcgcgtaagg ccgccgaaag cattgatccg gaaaaaatac gcgcccacgt gcgttttctt
       61 gcaagcgatc tgctcgaagg gcgcggcccc ggacagcgcg gcggacaact tgccgccgag
      121 tacattgcca cgcaattcgc gctcgatgga ttgaagcccg caggcgacaa cggaacatat
      181 ttccagaaag tcccgctcta tgctgtccac acggtggaag accagaccac agcggagttt
      241 gttcctgcaa gcggccagcc cctttcgctc gcgtacagca aagactgggt cgcgaaggac
      301 cagactgggc aagagtccgc cgatattgac gcgcccattg tttttgttgg ctatggcatt
      361 gatgctcccg aatatcactg gaacgactac accggcttag atgtgaaagg caaggttctt
      421 ctggtcatcg tgaatgagcc gccatccaac gatgagaaat tcttcaaagg caaggcgctc
      481 acctattacg gccgctggac ctataaatac gaagaagcgt cgcgccttgg cgcagccgga
      541 gtcctgatta ttcatcgcac cgatctggcc agctatccct ggcaggtggt gcgcgactcg
      601 caggccatcg aaaagtctta cctgcaggga gatcctgccg ccacgctcaa ggccgcggcc
      661 tggatccagc atgacgtggc acagaagctc tttaccgctg ccggactggg caacgtagac
      721 gatgagattg caagggccgg acagcgcggc ttccatgcag ttgaattgcc catacgcttc
      781 aaggcacaca tcgccagcaa agttcgccgc tatgagtcag acaatgtgat tgcagaggtt
      841 cccggcgcgc agcccggaca ggccgtcatc tataccgcgc attatgacca tctcggcatt
      901 gacccgaacg cgaagggtga caacatctac aacggcgcgg cggacaacgc gacaggctgc
      961 ggcatccttc tcgaactggc ccgcgcttat tctgagacaa ggccgccgca tccggtctat
     1021 tttgcttctg tcacggcaga agaacagggc cttctcggtt cgcagtggct gggaatgcac
     1081 ccgcctgtcc ctccatcaca gatcgctctc gacctgaact acgacatgct gaatccgatc
     1141 ggtattcctg attcgactga cgtcagcgga gcagagcgca ccactttcta tcctgaagtg
     1201 gagaagaccg caaaagactt ccatcttacc attgagccag accagttccc cggcgccggg
     1261 cactactatc gctcggacca cttcagcctt gcgcgcgtgg gcattccggc attttccatc
     1321 gggcagggaa cgacatttga aggccatgat cctgaatggg gcaagcaaca gatggaggat
     1381 tttgtagcgc accgctatca tcagccatcg gatgaatacc ggccagatat ggacttccgc
     1441 ggtgacgcca aaatggcgca gtttggattc attctcgggt gggaagcgat gtcgctgccg
     1501 aaagaaatcg gctggcagcc tggagatgaa ttcgaggcag cgcgaaagaa aggtcagtag
     1561 ctgaagccca catctctgcg agatgtggga cacccggcaa gaaagagcaa cagactgggt
     1621 tctaaaaacc gcacaatcat gaaatggcgt attcacattc tggcactgtt gttctcctcg
     1681 gcaagcatcc acgcccagac tgcgaatgcg cccaaactgc accaccgcgg agacccgagc
     1741 accaccgacc atcccatcgt cgtgaaacca gacaaaggca actctctgat tccggacgac
     1801 gcctccggcg aatatatgct gggcaagccc ggagagttca tcgaaatcac actgcaattt
     1861 ggcgacctca gcggatacat ctcccgccag ggagacagcg aaagcgacct gggcactcca
     1921 ttaacgttct tctttgaccg gacttcgcta caaggccagc aactgagttt caccacgcgc
     1981 caggtgcatg gcgtctggta ttcatttacg ggaacgattc agcgcggtat aggcaaaaca
     2041 cgtcaggaag acggctacta catgcttacc ggaaacttaa ttgagcataa tgccgcgcat
     2101 aatactgaag tccgccgcac ggtcgcgctc aaatcaggaa aacagttcgg ataaaacatt
     2161 ttacagatag tctggaccat gccacgaaac ctacagtggc tacacaagaa acctcttctg
     2221 actcgtgtac actaaagaag acccccgtgt cgggacgtgg ctcagcctgg tagagcactc
     2281 gcttggggtg cgaggggtcg gcagttcaaa tctgcccgtc ccgaccatct cctctctcca
     2341 ttcccagact ttccagaacg aagttaaaac cgcgactgct gctttacgtt tcttacagca
     2401 ccgatacgcc aaatcaaaat gagaaaatta aagttcatga aaaaaatcac tcttgaaatt
     2461 tgtctcgaat ccattgattc cgtaattgcc tccgaacgcg gcggtgcaca gcgagtagag
     2521 ctgtgcgcca atctgctgga aggcggaaca actcccagcg ccggaacgct tcgcgccgcg
     2581 cgcgagcgct ccagcattgc tatcaatgtc atggtccgcc cgcgcggtgg cgactttctc
     2641 tacacagaaa ccgaatttgc ttccatgcag catgatgttc gcatggcaaa ggacctgggc
     2701 gcagacggca tcgttctggg cctgctgcgc gcagatggca ctgtagacgt agaccgcacg
     2761 cgccagcttg tggaactggc agcgccgctg ccagtgacct ttcaccgggc gatagatgtc
     2821 tcgcgcgatc tgctggaagc gttggaagcc gtcgtctcca ccggagcagc gcgcgtactt
     2881 acttcaggcg gccagccctc cgtcgcagat ggagcgccaa tggtggcaaa gatggttgag
     2941 gccgctggag accgcattat cgtgatgccc ggatgcggca tcacaccgga caacgtgctt
     3001 tctattctgg aaacgactgg ggccagcgaa gtccacatcg cgctcgacga gcaagtccaa
     3061 agcccgatgg agttccgcaa aaccgaaatt ccaatgggag gcatggaagg acgcgaatat
     3121 ttgcgatacg ttacccctga gagcgcagta cggaaagtag cacagatttt aaggcgcaac
     3181 tgaactcgtg tgtgatgggc aacttcctgt atcactctga ctgctgaaaa aaggcgtttt
     3241 gtattttgac gactttacac acaagatagg ggaaaccttg tcaatctgcc ggaattacca
     3301 cacccctgca ttcgccaaag ccaatgcgtg gatgcccctc ccgctcgcaa tatccgcgca
     3361 ggacgacctc atcgccgtct tcgagaaatt tacgcctctc acccgtaggc aacgtgatgg
     3421 gttctgtacc actgcgggtc atctccagaa ggcagccgac agaatcccgc gttgcgccgg
     3481 agatggtccc ggtccccagg aggtcgccag gacgaagatt gcacccattg ctggtgtgat
     3541 gtgtaagcat ctgggcgagc gtccagtaga ggtcgcgcag attacctttg ctgattctga
     3601 ccgaagtggt cttttcatcg cgcatgagct ttgaggaaag ccagacctca actgtaatgt
     3661 caatgccgcc acgggtctgg tcgtctgatg aattgaggta cggcagcgga tcggggtctt
     3721 gctcctcgcg ccgaaaggcc gggacgcgga acggctcaag tgcgtccata gtgatgaccc
     3781 acggcgaaat ggtagtcgca aaactcttgg ccaggaatgg gccaagcggc tggtattccc
     3841 acgcctgaat gtcacgcgcc gaccagtcat tcaataggca aagaccgaag atgtgctctt
     3901 ccgctttgcc aatagggatg gtttgcccca gcgcgttgcc actgcttacg aagaagccga
     3961 cctccagctc atagtcaagc aggcgggatg gcccgaagcc gggaacggac atgtcggcag
     4021 acttgatttg gcccttgggc cgctttacat ccttgccgct gatcacaata gacgaggcgc
     4081 gtccatgata gccgatgggc acatacttgt agttgggcag cagcgggttg tccggacgga
     4141 agagcctgcc cacattggtg gcgtgataga tagaggcgta gaaatcggtg tagtcgccaa
     4201 tcgcagcagg caggtgcatc gtcactcgat caatggaaag cagcagcggc tcgattctgt
     4261 tgcgcagccc ggcaggtgca tcttttgcca gcagttccat cacgcgctgc cggacagcgg
     4321 aaacagtgtt cctccccagt gccataaggg aattcaacac tggttcagaa cacgcctttt
     4381 gcacgcggac gttgaggctg ttgaacagtc cctgctccac acaggcacgc agattgagca
     4441 cagaatcgcc tatggcgacg ccgatgcacg ggtccttccc cggcgttgaa aaaataccga
     4501 aaggaaggtt ctgcaatgga aaatcagtgc ggccgtcgtt ggccgaaaca acccagcttt
     4561 cccgcattaa agccatccca gggctagaag gtcattgaca ggctcagtga acga
//
LOCUS       DASRBV010000269         7187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_478857, whole genome shotgun sequence.
ACCESSION   DASRBV010000269 DASRBV010000000
VERSION     DASRBV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478857"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFA02_09870"
     CDS             complement(<1..474)
                     /locus_tag="VFA02_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10201.1"
                     /translation="MSKVWLIAKRLLWQNRWLFLLLMLWPYGMAVLLLAGGKPDPDDV
                     LWMLHQECFAGLALVAVTGSTLLGNEQRSRRIVTVLSRAVSRPMYLFALLVAAWIPLV
                     LYASGFLISGVALARAIHQSWGGVLVMLLLQLVPGVWAGAISIFWSVLFPPLIASL"
     gene            complement(471..1364)
                     /locus_tag="VFA02_09875"
     CDS             complement(471..1364)
                     /locus_tag="VFA02_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU10202.1"
                     /translation="MTAALEFLGTTKKFNSYLALDHLTLALQPGEILGFLGPNGAGKT
                     TAIHLAMGFLKPTSGGGMLSGRPFGDTAARERVGYLPDVPSFFAEGARSAVEFAGRLN
                     GMRNPRLRERARELLGRLDLLSAGKDARRFSRGMQQRLGLAQALVNDPEVLVLDEPTS
                     ALDPPGVMEVRELLRAARDEGKSIFFSSHQLSEVEQVCDRIAFLHKGKLLREGPLEKF
                     LRESGRSEIVLRGPALAGFQAYQSASQDGMLRYVVPTHMQRVLIEQAWMAGAELVSVA
                     PLRRTLEELFVCWSEGGSGRA"
     gene            1487..2608
                     /locus_tag="VFA02_09880"
     CDS             1487..2608
                     /locus_tag="VFA02_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HZU10203.1"
                     /translation="MATATTKAPGQADTAIPATMKAAVYRGVNDVRIETVPVPEIGPG
                     EVLVRVNSCGICGTDLKKIHTGSHSAPRIFGHETAGTIVKTGEGVEGFAMGDRVMVFH
                     HIPCRECYYCRKKTFAQCPVYKKVGCTAGFEPSGGGFAEYVRVMDWIVRSGGLVKIPD
                     GVSFEQAAFVEPVNTCLKAIVNLNLAPDETVLVIGQGPIGIMLAALAARTGAKVLTSD
                     LFAERHAIAAAYGLKHPIDAANENVIEVAKRESDGRGADAVILAVGGNALIRTAMDAA
                     RPGGRVLLFAQTQHGEAVIDPAAVCMDEKTLLGSYSASVDIQEEGIRLVFDGYSNGFD
                     LTRLISHRFPLEEAVEAIKIASNPNAGSMKIFIQPGLRK"
     gene            2614..3657
                     /locus_tag="VFA02_09885"
     CDS             2614..3657
                     /locus_tag="VFA02_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU10204.1"
                     /translation="MANTMTAAVLYGKEDLRIERLPVPSAGPGEIVVRIAAALTCGTD
                     LKVYRRGYHAKMLKPPIPFGHELAGTVEEAGEGVTKFRKGDRVVALNSAPCDQCYYCR
                     HGQQNLCDDLLFNNGAYAEYIRIPARIVEKNTLHIPEGVPFEHAALTEPLACVVRGLE
                     ETNPQPGDSIAVIGAGPIGLMFMHAAQLAGLQVIAVVKRDEQVEAAKVFGAEEVVQIT
                     AVEDVVAAVRALTPDHRGVDIAVEAVATPLAWQQAVAMVRKGGTVNFFGGCAAGTKVE
                     LDTNRLHYNDITLKATFHHTPGTARRAFDLITSGRFKCREYITGRARLADIGDVFRKL
                     MDRSNDIKTAIIP"
     gene            3663..4607
                     /gene="hpnC"
                     /locus_tag="VFA02_09890"
     CDS             3663..4607
                     /gene="hpnC"
                     /locus_tag="VFA02_09890"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZU10205.1"
                     /translation="MSVNAQTELIERGWAALPPEYRIPDSTPTLEEARAWCRRLAESH
                     YENFHVATWFLPKRLRPHFHSIYAYCRISDDLGDEVGNREQSLALLDLWQHELDACYR
                     GEARHPVFVALTETIHACDIPKQPFADLLVAFRQDQTITRFQTMEDVLAYCRYSANPV
                     GHLVLYACGYRDAERFRLSDHTCSALQLANFWQDVTVDYQKGRIYFPVADMQRFGVDE
                     ATIASRNFTPQFRELMRHEVDYARQMFKQGLPLIHMVDHELAMDLDLFSRGGLEILNA
                     IERQNYNVLRVRPAISKTRKVVLLLRALSGRFLGRRAA"
     gene            4604..5548
                     /locus_tag="VFA02_09895"
     CDS             4604..5548
                     /locus_tag="VFA02_09895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZU10206.1"
                     /translation="MTVSVEQAYAVCRQIARREAKNFYYAFVALPKPKRDAICAVYAF
                     MRHADDLSDDETKSREERRANLDAWLTAWHAAAKGGLTGDPVFIALRDAQARFSITTD
                     LLDQLVHGTAMDLHTNTKPPGEAVHPLRLDTYATFDDLYRYCYYVASVVGLVCIRIFG
                     YSDARAEKLAEETGIAFQLTNILRDVREDAERDRIYLPLEDLERFGVSINDILALKDG
                     HHMTLDQRELLEFQAKRAEHYYRSGEVLLPMVSPDARPALWVLITIYHRLLQRIERNN
                     FDVFSQKISVPTITKLIILARGMVRMFFERLRGIFLPS"
     gene            5623..7020
                     /gene="hpnE"
                     /locus_tag="VFA02_09900"
     CDS             5623..7020
                     /gene="hpnE"
                     /locus_tag="VFA02_09900"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZU10207.1"
                     /translation="MHANTKIESVIVVGGGVAGIAAASALADSGYRVHLAERKPYLGG
                     RASSYEHPGTGEIIDNCQHLLFGCCTNLLDVYRRIGALDKLRWFNEITMIEPGGRRTL
                     LRPSHLPAPLHASMAFLRAHAFSLADKLAIARGFSAFMTGLPEDTEENFAHWLVRHKQ
                     TPTAIKRFWEPVIYAALNEELDKTSVYYAAKVCRELFLRSPEAGRMAIPSVPLSELYG
                     HAMQSLQSRGAEVLLRTNITRVQWNEESRQWSVETETDRIQSDAVIFALSFEAMAKLL
                     PALPEVPGKEALAAQLAEFSHAPIAAIHLWFDREITDLLHASLLDTTIQWLFNKSKLQ
                     PQRHGREGHYIELVISVLRSVIPMQRQELIDLALRELALFFPAVRNATLLKAAVTKEV
                     RATFSVRPRIDRFRATAQSPWPHIFLAGDWTATGWPATMEGAARSGYLAAEAVSGQKF
                     LKPDVPSTGLMRLLG"
BASE COUNT         1620 a         2122 c         1954 g         1491 t
ORIGIN      
        1 cagcgaagca atcaacggcg ggaacagcac tgaccagaag atgctgatgg ccccggccca
       61 cacgccaggc accagttgca gcaggagcat caccagaacg cctccccatg actgatggat
      121 ggcacgggcc agggccactc cgctgatgag aaaaccgctg gcataaagga cgagcggaat
      181 ccacgcagcc acaagcagcg caaagagata cattggcctg cttacagcgc gcgaaagtac
      241 ggtcacaatg cgacgtgagc gctgttcatt gcccagcaga gtgctaccgg tgaccgcaac
      301 cagtgccagt ccggcaaagc actcctgatg tagcatccac agcacgtcgt cggggtcggg
      361 tttgcccccg gccagaagaa ggacggccat cccataaggc cacagcatca gcaacaaaaa
      421 cagccagcgg ttttgccata ggagccgttt tgcaatcagc cagactttgc tcatgcgcgg
      481 ccgctcccac cctcgctcca gcagacgaac agctcttcca gcgtacggcg cagcggcgca
      541 acgctgacca gctccgcgcc tgccatccag gcctgttcaa tcagcacgcg ctgcatgtgc
      601 gtgggcacaa catagcggag catcccatcc tgcgatgcgc tttgatacgc ctgaaagccg
      661 gccagcgccg gcccgcgcaa aacaatttcc gaacgcccgc tttctctcag aaacttctcc
      721 agaggccctt cgcgcagcag cttccctttg tgcagaaagg caatgcggtc gcagacctgc
      781 tcgacttcag ataattgatg ggagctgaaa aaaatgctct ttccctcatc gcgggccgcg
      841 cgcagcaact cgcgcacttc cattacccct ggagggtcga gcgccgaggt cggctcgtcc
      901 aggaccagca cctccggatc attcaccagc gcctgcgcca gtccgaggcg ctgctgcatt
      961 ccgcgagaaa agcggcgcgc atctttgccc gcggaaagaa ggtccaagcg gcccagcaac
     1021 tcccgtgctc tctcgcgcag acgtgggttc cgcatcccat tcagcctgcc cgcaaactca
     1081 accgcgctgc gggccccttc ggcaaagaac gacgggacat cgggcagata gccgacgcgc
     1141 tcgcgtgccg ctgtatctcc aaacggcctg cctgacagca tccctccacc cgatgtaggt
     1201 ttcaaaaatc ccatcgccag gtgaatggcc gttgtcttcc ctgctccgtt tggcccgaga
     1261 aagccgagaa tctcccccgg ctgcaatgca agcgtgagat ggtccagcgc cagatacgaa
     1321 ttgaacttct ttgtcgttcc caaaaactca agcgcggcag tcacggagga aattgtctca
     1381 tggcaaaaac accgcacaaa accgcgcaaa cagagttcca tctaaccagg aagtccggcc
     1441 ccgccgacgg attccagaaa gtcgcgataa tctgttaaaa gcgcttatgg ctacagcaac
     1501 cacgaaagca ccgggacagg ctgacacggc cattccagcc acgatgaagg ctgctgtgta
     1561 tcgcggcgtc aatgacgttc gtatagaaac agttcccgtc ccggaaattg gcccaggcga
     1621 ggtgctggtt cgcgtcaact cctgcggaat ctgcggaact gacctcaaga agatccatac
     1681 cggctcgcac tctgccccgc gtatcttcgg acatgagacg gcgggaacca tcgtcaagac
     1741 gggcgagggc gtggaaggct tcgctatggg cgaccgcgtg atggtcttcc accacatccc
     1801 atgccgcgaa tgttattact gccgcaaaaa gacctttgcc cagtgcccgg tttataaaaa
     1861 agtcggctgc accgcaggtt tcgagccttc cggcggaggc ttcgccgaat atgtccgcgt
     1921 aatggactgg attgtgcgct ccggcgggct tgtgaaaatc cctgatggcg tgtccttcga
     1981 gcaggctgct ttcgtagagc cggtaaacac ctgcctcaaa gccatcgtga acctgaatct
     2041 cgctccggac gaaaccgtcc tcgtcatcgg ccaggggcct atcggcatca tgctggccgc
     2101 gctggccgcc cgcaccggag cgaaagtgct cacctcggat ttgtttgccg aacgacacgc
     2161 cattgccgcc gcttatggtc tgaagcaccc aatagatgca gcaaatgaaa atgtcattga
     2221 agtggcgaag cgcgagtcgg atggccgggg tgccgatgcc gtgattctgg ctgttggcgg
     2281 caatgctctt atccgcacgg caatggatgc ggcccggccg ggaggccgcg tgcttctctt
     2341 cgcgcagacg cagcatggcg aagcagtcat tgatccagca gcggtctgca tggatgagaa
     2401 gaccctgctt ggctcctaca gcgcctcagt ggacattcag gaggaaggaa tacggcttgt
     2461 ctttgatggc tacagcaacg gatttgattt gacccggctg atttcacacc gcttcccact
     2521 ggaagaggct gtagaggcga ttaagattgc ctcaaacccg aatgccggtt ctatgaaaat
     2581 ctttattcag ccaggtttga gaaaatagaa gatatggcga acacgatgac agccgccgtg
     2641 ctttacggca aggaagacct gcgcatcgag cggctgcctg taccttctgc cggaccggga
     2701 gaaattgtcg tccgcatagc ggcagctctc acctgcggaa cagacctgaa ggtgtatcgt
     2761 cgcggatacc acgcaaaaat gctcaagcct cccatccctt ttggccatga gcttgccggc
     2821 acggtggaag aagcaggcga aggcgtgaca aagttccgta agggtgaccg ggtagttgcg
     2881 ctcaactcgg ccccatgtga ccagtgctac tactgcaggc acggccagca gaatttatgc
     2941 gatgatttgc tcttcaacaa cggagcgtat gccgaataca ttcgcatccc ggcccgcatc
     3001 gtcgaaaaga acacgctgca tattcctgaa ggagtaccct ttgagcacgc agcattgaca
     3061 gaaccgctgg catgtgtggt ccgcggactc gaagagacga atccgcagcc gggcgattcc
     3121 atcgccgtga ttggcgcagg accgattggg ctgatgttca tgcacgccgc acagcttgcc
     3181 gggctgcaag tcatagccgt tgttaagcgt gatgagcagg tggaagcagc aaaagttttc
     3241 ggtgcggaag aggttgttca gatcacagca gtggaggacg tcgtggccgc agttcgcgca
     3301 ctgacgccag accatcgtgg tgtggacatc gcggtagaag ccgttgccac tccgctggca
     3361 tggcaacagg ccgtcgcgat ggtgcgcaag ggcggtacgg taaatttctt cggtggatgc
     3421 gcggccggaa caaaagtcga gctcgacacg aaccggttac actacaacga cattacgttg
     3481 aaggccacat tccatcacac accgggaacg gcacggcgcg cattcgatct gattaccagc
     3541 ggacgcttca aatgccgcga atacattacc ggacgcgccc gcctggcgga cattggagat
     3601 gtcttccgta aactcatgga ccgctcgaac gacatcaaaa ctgcgatcat tccgtaagaa
     3661 aaatgagtgt aaacgcacaa accgaactga ttgaaagagg gtgggctgcg cttccaccgg
     3721 aataccgcat tcctgacagc acgcccacgc tcgaagaagc gcgtgcctgg tgccggcgcc
     3781 ttgccgaatc gcattacgaa aatttccatg tcgctacatg gtttctgcca aagcgtctgc
     3841 gtccgcactt tcacagcatc tatgcctatt gccgcatctc agacgattta ggagacgaag
     3901 tcggcaaccg cgagcagtcg cttgcgctgc ttgatctctg gcagcatgaa ctcgatgcct
     3961 gctatcgtgg tgaagcacgg cacccggtct ttgtggccct gactgagacc attcacgcat
     4021 gcgatattcc taagcagcca tttgccgatc tgctcgtcgc ctttcgtcag gaccagacca
     4081 tcacccgctt ccagacgatg gaggatgtgc tcgcctactg ccgctattcg gcaaaccctg
     4141 taggacatct tgtgctttat gcctgcggat atcgcgatgc tgagcgcttt cggctttcgg
     4201 accatacctg ctctgctttg caactggcga acttctggca ggacgtgacg gtggattacc
     4261 aaaaaggccg catctatttt ccggttgccg atatgcagcg ctttggcgtt gatgaagcca
     4321 ccatcgccag ccgaaacttt acacctcagt ttcgtgaact gatgcgccat gaagtggatt
     4381 acgcacggca gatgtttaag cagggattac cgctcatcca tatggtagac catgagcttg
     4441 ccatggacct tgacctcttc agtcgtggtg gtctggaaat cctgaatgcc attgagcggc
     4501 agaactacaa cgtattgcgc gtgcgtccgg cgatttccaa aacaagaaaa gtcgttctgc
     4561 tgctgcgcgc cctcagcggc agatttctgg ggcggagagc tgcgtgaccg tatctgtcga
     4621 acaggcgtac gcggtctgcc gccagatcgc ccgccgcgaa gccaaaaact tttattacgc
     4681 tttcgttgct ttgccaaagc cgaagcgtga cgccatctgc gctgtctatg cgttcatgcg
     4741 acatgccgat gatctttccg atgatgaaac caagtcgcgc gaagaacggc gcgcaaatct
     4801 cgacgcatgg ctcacggcat ggcacgctgc cgcaaaaggt ggcttaaccg gtgatcccgt
     4861 ctttattgca ttgcgtgatg cgcaggcgcg gttcagtatc acaacagact tgctggacca
     4921 acttgtacac ggcacagcaa tggacttgca cacaaacacg aagccgccgg gcgaagccgt
     4981 gcatccgctg cgcctcgata cgtatgcaac cttcgatgat ctctaccgct actgttatta
     5041 cgttgcttca gtcgtgggac tggtctgtat tcgcatcttc ggatatagtg acgcgcgggc
     5101 cgagaagctg gccgaagaga caggaattgc cttccagcta acaaacattc tgcgcgatgt
     5161 gcgcgaagac gcagaacgtg accgcatcta tctcccactt gaggacctgg agcgcttcgg
     5221 ggtttcgata aatgacattc tggcccttaa agacggtcat cacatgacgc tggaccagcg
     5281 tgaattgctg gaatttcagg cgaagcgcgc tgagcattac taccgttcgg gagaggttct
     5341 gttgcctatg gtttctcccg atgcgcggcc tgctctgtgg gtactcatca cgatttacca
     5401 ccgcctgttg cagcgcatcg agcgaaacaa ttttgatgtt ttttcgcaga aaatctccgt
     5461 tccaacgatt acaaagctca tcattctcgc acgcggaatg gtacgcatgt tcttcgagcg
     5521 cttgcgagga atttttttgc cgtcctgagc ggagtgaaac aaagaaaagc aatcgcgaaa
     5581 gacctgcatt tacgatgctt ccctgatgta agaaactgac gcatgcacgc aaatacaaaa
     5641 atcgaatcag tcatagttgt cggtggaggt gttgccggca ttgccgcagc ctctgcactc
     5701 gccgactccg ggtatcgcgt gcatctggca gagcgcaagc catatcttgg cggacgcgca
     5761 tcctcatacg agcatccagg tacaggcgaa atcattgaca actgccagca ccttcttttc
     5821 ggctgctgca ccaacctgct tgatgtttac cgccgcattg gagcgctcga caaactgcgc
     5881 tggttcaatg aaatcacaat gattgaacct ggaggccgca ggacgctgct tcgcccctcg
     5941 catctgcctg cgcccctcca tgccagcatg gcctttctgc gcgcgcatgc attttccctg
     6001 gcagacaaac tggccattgc gcgcggattc tccgcattca tgactggcct gcctgaggac
     6061 acggaagaaa actttgcgca ctggctcgtg cgtcacaagc agactccaac ggcgatcaag
     6121 cggttctggg agcctgtcat ctatgctgcg ctgaacgaag aactggacaa aacctcggtc
     6181 tactacgccg caaaggtctg ccgtgaactg tttctgcgct cgcccgaggc gggccgcatg
     6241 gcaattcctt ccgttccttt aagcgaacta tatggtcatg ccatgcagtc gctccaatcg
     6301 cgcggggcag aagtgctgct gcgcaccaat ataacgcgtg tgcaatggaa tgaagagtcc
     6361 aggcaatggt ctgtagaaac agaaacagac cgcattcaaa gcgatgcagt catctttgcg
     6421 ctttcctttg aagccatggc aaaactgctg cccgcgctgc cggaagttcc cggaaaagag
     6481 gccctcgccg cccagcttgc agaattcagc catgcaccga ttgcagccat ccatctctgg
     6541 ttcgatcgcg agattacgga tctgttgcac gcgtccctgt tggatacaac catccaatgg
     6601 ctattcaaca agtcaaagct gcaaccgcag cggcacgggc gcgaaggaca ttacatcgag
     6661 ctggtcatca gcgtgctccg gtccgtcatt cccatgcagc gccaggaatt gattgatttg
     6721 gccctgcgcg aactggcgct cttctttcct gcggtacgca atgccacgct gctaaaggcc
     6781 gccgtgacca aagaagtacg cgcaacattt tctgttcgtc cacggattga caggttccgt
     6841 gccacagcac aaagcccctg gcctcacatc ttccttgcag gtgattggac cgccactggt
     6901 tggcccgcca cgatggaagg cgcggcacgc agcggctatc tcgcagcaga agctgtaagt
     6961 ggacagaagt ttttgaagcc cgatgtaccg tccaccggac ttatgcgcct cctcggatag
     7021 ttcccctgga tgctgtcctc ctaagcaaga cggagaagga cttgcttctt catcgcaatg
     7081 ccgcaggtcc tttgctgatt tacactgaca cttactccat tattcatgcg tgaaagcctt
     7141 gaattcgcca tcgtctggct gctggtgcgc attcttggcg cgcttcc
//
LOCUS       DASRBV010000270         4551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_16895,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000270 DASRBV010000000
VERSION     DASRBV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16895"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="tuf"
                     /locus_tag="VFA02_09905"
     CDS             complement(<1..918)
                     /gene="tuf"
                     /locus_tag="VFA02_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZU10208.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHIDHGKTTLTAAITKVLSKHNPNIK
                     FRSFDTIDNAPEERERGITIATAHVEYETPNRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTKEHVLLARQVGVPYIVVFLNKCDAVEDPELIDLVEMEVRELLN
                     KYNFPGDDVPVVRGSALGALNGEAQWEAKIDELMEAVDKNVPLPQRAVDQPFLMPIED
                     IFSISGRGTVVTGRIERGKVKVGEEVEIVGFRETRKTVVTGVEMFKKQLDEGLAGDNA
                     GLLLRGTAKDEVERGMVLAKPGSITPHTKF"
     gene            complement(937..1230)
                     /locus_tag="VFA02_09910"
     CDS             complement(937..1230)
                     /locus_tag="VFA02_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZU10209.1"
                     /translation="MVVWEFQVRPGCEGAFREAYAPNGVWARFFRRSTEFLGIDLVRS
                     VRHPARFFSLGAWTSEEAFRSFCEQNGESFEKLDVKLAGLTEWERRIGAFPSE"
     gene            complement(1271..1579)
                     /locus_tag="VFA02_09915"
     CDS             complement(1271..1579)
                     /locus_tag="VFA02_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10210.1"
                     /translation="MFEQAVLPVQDEKTFAEVERALEEAFAPANVAKFLRQVEKAKLR
                     VRQFEAILAHGLLGANTPAQYGSLGDADRGQVREKYLRLVEKVAPELRAKYLKVYAYY
                     "
     gene            complement(1608..1682)
                     /locus_tag="VFA02_09920"
     tRNA            complement(1608..1682)
                     /locus_tag="VFA02_09920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1648..1650),aa:Gly,seq:tcc)
     gene            complement(1799..1874)
                     /locus_tag="VFA02_09925"
     tRNA            complement(1799..1874)
                     /locus_tag="VFA02_09925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1839..1841),aa:Thr,seq:ggt)
     gene            complement(1883..2071)
                     /locus_tag="VFA02_09930"
     CDS             complement(1883..2071)
                     /locus_tag="VFA02_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10211.1"
                     /translation="MRVRGLQRKVVALLALAVLAALAWSTMDAGKVRVLVFVLLGGFA
                     LRILLTARSRYDEEGHSD"
     gene            complement(2095..2182)
                     /locus_tag="VFA02_09935"
     tRNA            complement(2095..2182)
                     /locus_tag="VFA02_09935"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2146..2148),aa:Tyr,seq:gta)
     gene            complement(2319..2394)
                     /locus_tag="VFA02_09940"
     tRNA            complement(2319..2394)
                     /locus_tag="VFA02_09940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2359..2361),aa:Thr,seq:tgt)
     gene            2668..3612
                     /locus_tag="VFA02_09945"
     CDS             2668..3612
                     /locus_tag="VFA02_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10212.1"
                     /translation="MYSQHFRLRFTRKNAAKLLLLNIFLLGAAVSCRTSQDAQTAATR
                     LTATAEDLHLYYVSLSKVVEQHEKLERLQNALLGVPLDQQDLDQLKTAHEEIEKRAEI
                     AKSLADLAQAYSELTNSQAAEKVALSAENLGSALSGIKQLPGSSHAPEALKRAGNLLV
                     TLAQKHDQRKMAQAMDPTITGLSEMFSEEKPVYGSIHRTYIGLAQSLALELVRRHQID
                     ARSLLLPALRPFGLPPGESGENLSPGLQAYAQQQIQERGQSEIAAHNAASTYLDEALK
                     EAAKQIHALASGEHLPPAPPQPYKSSVERWTAMLSEKE"
     gene            complement(3688..>4551)
                     /locus_tag="VFA02_09950"
     CDS             complement(3688..>4551)
                     /locus_tag="VFA02_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10213.1"
                     /translation="GLPMGVLNASTTLGQAMAPPVLTLLMVAYGWRSMFVWIGVLGVA
                     VAVVWFPTYRGPARFTSPRDAEHPGPQPPIWPSALDDKVTLRAWLRLFRQRTLWGMML
                     GFSGVNYTAWLYLAWVPGYLEAAHHVGLKATGWLAAVPFLMGSAGMLANGVVADGLVR
                     KGRDPLRSRKALIVAGMVCSAACTMLVPRAQTGAGSAAVIGMALFFIHFAGTSAWGLV
                     QTAVPAHLVATVGATQNFCSFVFASAAPVVTGWFLDRTHSFNIALMICAGVTLLGAIA
                     YWWLVKDGISG"
BASE COUNT         1035 a         1581 c         1061 g          874 t
ORIGIN      
        1 gaacttcgtg tgcggcgtaa tcgaaccggg cttggccagc accatcccgc gctccacctc
       61 gtccttcgcc gtgccacgca gcagcaaccc ggcattgtct ccagccaggc cctcgtccag
      121 ctgcttcttg aacatctcta cacccgtgac tacagtcttt ctggtctcgc ggaagcccac
      181 aatctccact tcctcgccca ccttcacctt gccccgctct atccggcccg tcaccaccgt
      241 cccacggccc gagatcgaga agatgtcttc tatcggcatc aggaacggct ggtccaccgc
      301 gcgctgcggc agaggaacat tcttgtccac cgcctccatc agctcatcta tcttcgcttc
      361 ccactgcgct tccccgttca gcgcgcccaa agccgagccg cgcaccaccg gcacatcgtc
      421 cccagggaag ttgtacttgt tcaaaagctc ccgcacttcc atctcgacca ggtctatcag
      481 ctccgggtct tccaccgcat cgcacttgtt cagaaacacc acaatgtacg gcacgcccac
      541 ctgacgggcc agaagcacgt gctccttggt ctgcggcatc ggcccatcgg tcgccgccac
      601 caccagaatc gcaccgtcca tctgcgccgc gcccgtaatc atgttcttga tgtagtccgc
      661 gtgcccaggg cagtccacgt gcgcatagtg acggttcggc gtctcatact ccacgtgcgc
      721 cgtcgcgatc gtaataccgc gctcccgctc ttccggcgca ttgtcaatcg tgtcaaacga
      781 gcggaacttg atgttcgggt tgtgcttcga caacaccttc gtgatcgccg ccgtcaacgt
      841 cgtcttccca tgatcaatgt gccctatcgt ccccacattt acgtgcggct tcgaccggtc
      901 aaatttctcc ttcgccatct tgcgagtctc ctcaatttac tcggagggaa atgcgccgat
      961 ccggcgctcc cattccgtta atcctgccag ctttacgtcc agcttctcga aactttcacc
     1021 attctgctcg cagaagctgc gaaacgcctc ttctgaagtc cacgcaccca gactgaaaaa
     1081 ccgtgccgga tgcctgacgc ttcgcactaa atctatcccc aggaactccg tagacctgcg
     1141 gaaaaacctg gcccagacac cgttcggcgc ataggcctcc cgaaacgccc cttcgcagcc
     1201 gggacgcacc tgaaactccc acaccaccat gtaaacgccg gatacggaag cctgtgcgaa
     1261 tgcaaatccc tcagtaatac gcgtacacct tcagatattt tgcgcgcagc tcaggagcaa
     1321 ccttctccac taacctgaga tacttctcgc ggacctggcc ccggtctgca tctcccagag
     1381 aaccatactg cgccggagta tttgctccca acagcccatg cgccagaatc gcctcaaact
     1441 gccgcacacg caattttgcc ttttctacct gacgaagaaa cttcgccaca tttgccggag
     1501 caaaggcctc ttcgagcgcc ctttccacct cagcaaaagt cttctcatcc tgaaccggaa
     1561 ggaccgcctg ttcaaacatg cttgctgcgt actccgtctt caacttctgg agcgggagac
     1621 gggaatcgaa cccgcgacca acagcttgga aggctgtgac tctaccactg agttactccc
     1681 gcgcaatatc agcaagccag cccgcctccg gcggaagcga gttagctggg caatccatat
     1741 tcctgccgac ttgctgaccc gcccgcgcct gccaggcgcg ctgacttgct caaaatcctg
     1801 gagctgatga ccgggattga accggtgacc tctcccttac caagggagtg ctctaccaac
     1861 tgagctacat cagcctgaaa acctaatcgg agtgcccttc ttcatcgtac cgcgaacgcg
     1921 cagtcaacag aatgcgaaga gcaaacccgc ccaacaggac aaacaccagc actcttacct
     1981 tgccggcatc catcgtgctc catgccaggg ccgcgagcac cgccaaagca aggagcgcca
     2041 ctaccttgcg ctgaaggccc cgcacacgca tgtgattccc tgaaaaactt taactggtgc
     2101 acaggggagg attcgaacct ccgtagctcc aaggagcggc agatttacag tctgctgcca
     2161 ttaaccactc ggccacctgt gcatccattt tttgcgcatc ctcagcccca tccagacgcc
     2221 agccgtcttc agcgaagccc acaacatgct ccggcttgaa acgggacgct gtcaggtgga
     2281 atgactgcgg attcgcgcct tacgaggctg gatttttctg gagctggcga agggatttga
     2341 acccccgacc ctctgattac aaatcagatg ctctaccagc tgagctacgc cagcccgatc
     2401 agccacatac tccaattccg agagacacta atgcgtctgc tgaaagagtc gtacagaagt
     2461 aaagattaac acaccgaatg atgcctcgca atgcatcacg taagaagtta aagcgattga
     2521 taattaccac ttcagctatg atatgcatgt ccgcaatcca tccgtcgtca caaactcctg
     2581 acatcaggca gcctcccttc gccgcctttc ttcgcctttg agcattccat cgtgtcctgt
     2641 tatgctttct taccctgaga agttgctatg tactcacagc attttcgcct ccgattcacc
     2701 cgaaagaatg ctgcgaaact actcctgcta aacatcttcc tcctgggggc cgcagtttct
     2761 tgccgaacat cgcaggacgc ccaaaccgca gccacccgcc tgactgcgac agcggaagac
     2821 ctgcaccttt attacgtctc tctcagcaaa gtcgtggaac agcacgagaa actcgagcgc
     2881 ctccagaacg cgctgctcgg cgtgcctctc gaccagcagg acctcgacca gttaaagacc
     2941 gcgcatgaag aaatcgagaa gcgcgcggaa atagcgaaat cacttgctga cctcgcccag
     3001 gcgtactctg agcttacaaa ttcgcaggcg gctgaaaaag tagcactatc tgctgaaaac
     3061 ctcggcagcg cattgtcggg aatcaagcag cttcctggct cctcccatgc tccggaggcg
     3121 ctgaagcgcg ccgggaatct gcttgtcacc ctcgcccaaa agcatgacca gcgcaaaatg
     3181 gcccaggcca tggatccaac catcaccggg ctttccgaaa tgttctcaga ggaaaaaccc
     3241 gtctacggct ccatccatcg cacatatatc ggactggccc aaagtctcgc ccttgagctt
     3301 gtgcgccgcc atcagataga tgcgcgcagc ctgctgctcc ccgcgctgag gcccttcggt
     3361 cttccgccag gtgaatccgg agaaaatctc tcgcccggac tccaggccta cgcccaacag
     3421 cagattcagg agcgcggaca atctgaaatc gctgcccaca acgcagcttc aacctatctg
     3481 gacgaagcgc tcaaagaggc cgcaaagcaa atccatgcgc ttgcctcggg cgaacacctg
     3541 ccgcccgcgc ctccgcagcc gtacaaatcc agcgtcgaac gctggaccgc aatgctctct
     3601 gaaaaagaat gactgccagc caaatgcagg gatgtctgaa attcctcatc ctatcgcatc
     3661 atttcgcata cggcagatgt acggccctca cccggaaatg ccgtccttca ctaaccacca
     3721 gtacgctatt gcgcccagta acgtcacgcc ggcgcaaatc atgagagcaa tattgaatga
     3781 gtgagtgcgg tccagaaacc atccagtcac taccggcgcc gccgacgcaa acacaaagct
     3841 gcaaaagttc tgggtagcgc ccaccgtcgc caccagatgc gcaggcaccg cagtctgcac
     3901 caggccccag gccgaggtcc cggcaaagtg aataaagaaa agcgccattc caatcacggc
     3961 cgccgatccc gctcccgtct gcgctcgcgg aaccagcatc gtgcatgctg ccgagcagac
     4021 catccccgca acaatcaggg ccttgcggct gcgcagcggg tcgcgtccct ttcggacaag
     4081 cccgtccgcc accaccccat tcgccagcat gcccgcagac cccatcagaa aaggcaccgc
     4141 cgccagccat cccgttgcct tcaaacccac atggtgcgcc gcctccagat atccgggcac
     4201 ccacgccaga taaagccacg ccgtataatt cactccactg aaccccagca tcatccccca
     4261 cagtgtccgc tgacggaaaa gccgcaacca tgcccgcagc gtgactttgt catccagggc
     4321 cgaaggccag ataggaggct gcggacctgg gtgttccgca tctcgtggcg atgtgaaacg
     4381 ggcaggcccc cggtaagtgg gaaaccacac caccgcaacc gccaccccca gtactccgat
     4441 ccacacaaac atgctgcgcc atccataggc caccatcaac agtgtcagta ctggaggggc
     4501 catggcctgg cccagcgtcg tcgaagcatt cagcacaccc atgggcagcc c
//
LOCUS       DASRBV010000271         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_731696, whole genome shotgun sequence.
ACCESSION   DASRBV010000271 DASRBV010000000
VERSION     DASRBV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731696"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..563)
                     /locus_tag="VFA02_09955"
     CDS             complement(6..563)
                     /locus_tag="VFA02_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706992.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HZU10214.1"
                     /translation="MPHIALPEGLPGISAGFAFRPETAKPMRELAHILLHEPNSLTPA
                     ERELIATYVSYLNDCYFCQTSHGAAAAAHLGDGDLVKRVKADFQHADISDKLKALLVI
                     AGKVQKGGKKVTAKDVEEARAQGATDMEIHDTVLIAAAFCMYNRYVDGLGTWQPRDES
                     LYAAMGKHLATEGYRKPSIERAEVA"
     gene            complement(579..1151)
                     /locus_tag="VFA02_09960"
     CDS             complement(579..1151)
                     /locus_tag="VFA02_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680833.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HZU10215.1"
                     /translation="MPYIQLPEGLPGIRGAMAFRPETAKPLNELVEVLLHSPGTLTPG
                     ERELIATYVSYLNDCYYCQTIHGAIAAASLNDDEELVKRVKSDFQHADISDKLKALLV
                     IAGKVQKGGKHVTSEDVEAARHLGATDLEIHDTVLIAAAFCMYNRYVDGLGTWQPRDE
                     AMYRERGKRIARDGYVSISKEYLPAEAAAH"
     gene            complement(1164..2486)
                     /locus_tag="VFA02_09965"
     CDS             complement(1164..2486)
                     /locus_tag="VFA02_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU10216.1"
                     /translation="MEPKRQMGLAAAIAAVTGEAVALGIFLTPAAMAKSLGSPLLLAA
                     VWCGIAMVALCGALCYSELAVRFPLAGGEYVYLREGYGPRVAFLYGWMSAAVIDPGIA
                     AALSVGAVPYVQSIAAVSPRMAALLPFAFLICLGIINYAGTRLSSRIMATANLLKIAV
                     LFALVAWAFLSGHAFGNNLLPLVARRSGSGPLLGAIAGATVNAFFSFGGWWEAGKLAG
                     EVRNPRRTLPMAFVCGVLLVTAVYLTISFAFLCVVPLERIVSNTAFVAQFGEALFGHA
                     GGAILSACVLLSVAGGLMAMTMAAPRVYYAMARDGAFFPAFGKLHPRFGTPANAVLLQ
                     TGMALLVLCFGAFDRILAYIIFSAVCFLSLSAASLFRLREPVRRWWYPTAPVVFLAGS
                     GVIALLVLLHNPLPALIGVVVVLSGDLLRRWLVPAKPFPAEVVEPTIS"
     gene            complement(2549..3787)
                     /locus_tag="VFA02_09970"
     CDS             complement(2549..3787)
                     /locus_tag="VFA02_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10217.1"
                     /translation="MLGDLWGQAYDAMLYNRRRTAITIVGMAWGIATVVLLLAYGAGF
                     GRAIEAIFAQWGTQLIGVFPGLTSEQAGGTKAGVQVRFTQDDVDRLWNSVPNLQHISP
                     MLSKQVPVANDLHTYTWEVNGYRAGIQDILKLDINSGRFFTNLDDQQRSHVAVIGSEA
                     KTKLFSGQYAVGQHIRINGISFIVIGVLKPKMQEGDDDVNRQVYVPFNTMGDIKDLKY
                     LDGIWMNYTGDHMLVEKAIRNTLGAAHNFPPSDHNAIFVANLMEQLSQFRIITIALQI
                     LLLFIGALTLGIAGIGLMNIMLVAVQQRTREIGIEKALGARKRHILIQFLAESLVITG
                     VGGAIGIALAYAVSIGVGRITFYSALASNAEAADIRLLISPMIVLVATSILVIVGLVS
                     GMVPAIKAANLNPIEALRYE"
     gene            complement(3796..>4252)
                     /locus_tag="VFA02_09975"
     CDS             complement(3796..>4252)
                     /locus_tag="VFA02_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU10218.1"
                     /translation="NEWDTIKTEQMVGKIFTAMDVFLGGVGIVTLALGAVGIINIMLV
                     SVTERTSEIGLRKALGATRRNILTQFFIEGLLLTGVSGLAGIGGAAVFMYMLHAAVGD
                     NMRGFDPPHMAPWSAALALGALGLSGIIAGLYPAGKAAELDPVEALRRE"
BASE COUNT          989 a         1209 c         1188 g          866 t
ORIGIN      
        1 tctccttatg cgacttcagc ccgctcgatg gagggcttgc gatagccttc cgtggcaagg
       61 tgtttgccca tcgccgcata gagagactca tcgcgcggct gccatgtgcc gaggccatcc
      121 acatagcggt tgtacatgca aaaagcggcg gcgatgagga cggtgtcgtg aatctccatg
      181 tccgttgcgc cttgcgcgcg ggcctcctct acatcctttg ctgtcacttt tttaccgccc
      241 ttctgcactt tgccggcgat gacgagcagg gcctttagtt tgtctgagat gtctgcgtgc
      301 tggaaatcgg ccttgacgcg tttcaccaga tctccatcgc caagatgggc cgctgctgcc
      361 gcgccgtggc ttgtctgaca gaagtagcag tcattcaggt aagaaacgta ggtggcaatc
      421 aattcccttt ccgcgggggt gaggctgttg ggttcgtgca ggagaatgtg cgccagttcg
      481 cgcatgggct ttgccgtttc ggggcgaaac gcgaacccgg cactgatgcc gggcagtcct
      541 tcgggaagtg caatatgtgg catggctttt tctccttttc agtgggccgc ggcttcagcg
      601 ggcagatact ctttgctgat ggatacatat ccatcacggg cgatgcgttt accgcgctcg
      661 cggtacattg cttcatcgcg cggctgccaa gtgccgagac catccacgta acggttgtac
      721 atgcagaagg cagcagcaat cagcacggta tcgtgtatct caagatcggt agcgccgagg
      781 tggcgcgcag cttctacgtc ttctgaagtt acgtgcttgc cgcctttctg tactttgccg
      841 gcgatgacga gcagggcctt cagtttgtct gaaatgtctg catgctgaaa gtcagatttg
      901 acccgtttga ccagctcttc gtcatcattc aagctggcgg ccgcgatggc cccgtggatc
      961 gtctgacagt aatagcagtc gttgagatat gagacgtagg tagcaatcaa ttcacgctca
     1021 cctggagtca gcgtgccggg actgtgcaac agcacttcga ccagttcatt caatggcttc
     1081 gcagtttccg gacggaaggc catcgcccca cgaattccgg gcagcccctc gggtagttga
     1141 atgtatggca tggcaacact cctttatgaa atggttggct ccacgacctc ggccgggaac
     1201 ggctttgcag gtacgagcca ccggcgcagc aggtcgccac tgaggaccac tacaacgcca
     1261 atcagcgccg gcagtggatt gtgcaggaga acaagcagcg caattacacc actgcctgca
     1321 agaaaaacaa ccggagccgt tggataccac cagcgccgca ccggctccct gagccggaag
     1381 agcgaggcag cggaaagcga gagaaaacac acggcggaaa aaatgatgta agccaggatg
     1441 cgatcaaatg ctccgaagca aagcaccagc agggccattc ctgtttgcag caggacggca
     1501 tttgcaggag tgccgaaacg ggggtgcaat tttccaaaag ccgggaaaaa cgcgccatct
     1561 ctggccatgg cgtagtagac gcgtggggcc gccatggtca tggccatcaa tccaccagcg
     1621 acagacaaca gcacacaggc ggacaaaatc gcccctccag catgaccgaa gagcgcttct
     1681 ccaaactggg cgacaaatgc tgtatttgag acgatgcgtt ccaacggcac aacacataaa
     1741 aacgcgaagc tgatggtgag gtagacggcc gttaccagca gaactccgca aacgaatgcc
     1801 atgggcagcg ttcgcctggg gttccgtacc tcgcctgcga gtttgcctgc ctcccaccat
     1861 ccgccaaaac tgaagaatgc gtttacggtg gcgccggcaa tggcacccaa cagtggccct
     1921 gacccggacc gccttgcaac cagtggcagc aggttattgc cgaaagcgtg cccggagaga
     1981 aatgcccatg ccacaagcgc aaagagtaca gcaattttca gcagattggc tgttgccatg
     2041 atgcgactgc tcaggcgggt cccggcataa ttgatgatgc ccagacagat gagaaaggca
     2101 aatggaagaa gagcagccat acgcggagaa accgccgcga tggattgcac ataagggaca
     2161 gcgcctacgg acagcgccgc agcaatgccc ggatcaatga ctgctgccga catccatcca
     2221 taaagaaaag cgacacgggg accatagcct tcgcgcagat acacatattc tccgcctgcg
     2281 agcggaaaac gcacggccaa ctcggaatag caaagggcac cgcaaagcgc gaccatggcg
     2341 attccgcacc agacagcggc cagcaatagt ggagaaccaa gggattttgc catagccgca
     2401 ggagtgagaa agatgccgag agcaacggct tcccctgtga cggcagcaat ggctgcggcc
     2461 aggcccatct gtcgttttgg ctccatgcgg aagccatgct atctggggcc agcaacaaca
     2521 aacatccacc aaaaggtgga aactgggttc actcgtagcg aagagcttcg atgggattga
     2581 ggttcgcggc tttgatggcg gggaccatgc cgctgacgag tcccacaatg accaggatgc
     2641 tggtggccac aagcacaatc atgggagaaa tcagcagccg tatgtctgct gcttcagcat
     2701 tcgaggccag ggcgctgtag aaggtgatgc ggcctacgcc aatcgaaacc gcataggcca
     2761 atgcgattcc aatggcaccg cccacgccgg ttatgaccag tgattcagca agaaactgga
     2821 tgaggatgtg ccgcttgcgg gccccgagtg ctttttctat accgatttcc cgggttcgct
     2881 gctgcacggc gacaagcatg atgttcatca gaccgatgcc ggcaattcct aaagtcagcg
     2941 cgccgatgaa aagcagcaat atctgcaaag caatcgtaat gatgcggaac tggctcaact
     3001 gctccatgag gttggcgaca aaaattgcat tgtgatcgga tggcggaaag ttgtgggccg
     3061 cgcccagtgt gttccggatg gccttttcga ccagcatgtg gtcgcccgtg tagttcatcc
     3121 atattccgtc aagatacttc aggtccttga tgtcgcccat cgtattgaac ggaacataga
     3181 cctggcggtt tacatcatcg tcgccttcct gcatcttggg cttgagcact ccaatcacga
     3241 tgaaactgat gccgttgatg cggatgtgct gcccgactgc atactggccg gaaaagagct
     3301 ttgtctttgc ctcagacccg atcaccgcca catggctccg ctgctgatca tcaagattgg
     3361 taaagaacct tccggaattg atgtcgagct tgaggatgtc ctgaatgccg gcgcggtagc
     3421 cattcacctc ccatgtatag gtatgcaggt cattcgctac gggcacctgc tttgagagca
     3481 tgggcgaaat gtgctggaga tttggcacgg agttccagag acggtctacg tcatcctggg
     3541 taaagcgcac ctgaacgccc gcctttgttc ctcctgcctg ttcgctggta aggcctggaa
     3601 aaacaccgat cagttgcgta ccccactggg caaagatagc ttcaatggca cggccaaatc
     3661 ctgcaccata cgccagaagt aacactacgg tagcaatgcc ccacgccata ccgacgatgg
     3721 tgatagctgt acggcggcgg ttatacagca tggcatcata ggcctggccc cacaaatcgc
     3781 ccagcatcgt tgctcctatt cgcgccgcag cgcttctacc gggtccaact ctgcggcttt
     3841 gcctgcaggg tagagtccgg cgatgattcc gctcaggccc aaagcgccca atgccagcgc
     3901 cgctgaccag ggcgccatat gcggaggatc gaagccccgc atattgtccc ccaccgcagc
     3961 atgaagcata tacataaata cagccgcgcc gccaattccg gcgagtccgc ttacaccggt
     4021 gagcagcaat ccttcgataa agaactgggt caggatgttt cgcctggttg cgccaagagc
     4081 tttacgcaaa ccaatttcgc tcgtccgttc tgtgactgag acaagcatga tattgatgat
     4141 gcctactgcg cccaatgcca gcgtcacaat gcccacgccg cccaaaaaga catccatcgc
     4201 cgtaaatatc ttccccacca tctgttcggt cttaatggtg tcccattcgt tg
//
LOCUS       DASRBV010000272         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_119174, whole genome shotgun sequence.
ACCESSION   DASRBV010000272 DASRBV010000000
VERSION     DASRBV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119174"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFA02_09980"
     CDS             <1..471
                     /locus_tag="VFA02_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU10219.1"
                     /translation="VSLEVYYSRRLHLTSLEAAVLKPLLVSSALLLVALFSAAQDQPS
                     SSQQPSSSESIPQTNPVKPTPQSQARAKQIYGYDCAMCHGDKGDGKGDVANDMKLTLR
                     DYTKPDALKDLTDAQMYDIIKNGKGQMPGEGPRAKPDEIWNLIIYLRSMSKKTA"
     gene            complement(620..1399)
                     /locus_tag="VFA02_09985"
     CDS             complement(620..1399)
                     /locus_tag="VFA02_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZU10220.1"
                     /translation="MKNKKPELEKSIDPGSAESFIAEKHIGERIKRLRLKKSMGLVEL
                     GRHTGLSASFLSQLETGRVVPTLRNLARIAMVFGKDLSYFFETEPHTVFRIHKKGERV
                     RLPQTGVDDPTYYFESMGFMVPDRQLDPYYAEFIPVKKPVPIQSHVHPGYEFLYVLEG
                     DLEIRHGEKVHVLEAGDGVYFDASTPHSYRCAGKASAKAIIVSMHQPAVHQPAMHLRP
                     MGTPVGAQVPASQPNKTASAAPPPPQRPLVSQQIPELRAKA"
     gene            1577..2467
                     /locus_tag="VFA02_09990"
     CDS             1577..2467
                     /locus_tag="VFA02_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZU10221.1"
                     /translation="MRVLISGASGLIGSSLARELAYRGAGVFRLVRRRPEDAREVALP
                     PQGHPAQFEDFTAVIHLAGANIAAHRWTEAYKREIAESRIQPTAKLAHVLAGLKRPPQ
                     VFLCASAIGIYGDRGEEALAEDSPAGTGFLAETCRAWEAAAQPARDAGIRTAHMRFGV
                     VLARKGGALDKILPVFQLGAGGRLGSGKQWMSWISLQDAISAIAFLMEKETIYGPVNV
                     VAPAPVTNAEFTQLLARMLHRPAIFPVPAFALRVALGQMAEEGLLASARVIPQKLQQA
                     GFRFEHPQLADALSATLKRE"
     gene            2512..3549
                     /locus_tag="VFA02_09995"
     CDS             2512..3549
                     /locus_tag="VFA02_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HZU10222.1"
                     /translation="MFPLLISCGLESPPRAPSLYLPQPPTDLTAYRSGNDVYLHWTMP
                     KRSTDRVPLQGGQQVMICRSFAGDPCETAGKARYAPGTAADFTDHLPAGLVSGAPQLL
                     TYTVEITNKKGHAAGPSNPAYTASGTAPAALTDLSAEVHAEGVLLRWQPAQGPGNLVR
                     LNRILTNPPKRSSSPMAGAPAPAEQKLETDYVSGHDPGATLDKDAALDHIYRYTAQRI
                     AFFEFGGHKVEVASTPSETIVVNARDVFPPAVPSGLVAVANPEGHSIDLSWTPDAEND
                     LAGYIVYRREAGSSAGPVRISPPEPVAGPAFSDTTAKPGVRYAYSVSAVDKDHNESAR
                     SAEVEEALPKF"
     gene            3619..>3996
                     /locus_tag="VFA02_10000"
     CDS             3619..>3996
                     /locus_tag="VFA02_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU10223.1"
                     /translation="MRYCRFQTKAGPQYGEVADRNGTLWIERLLPPFEEDPWTKLDET
                     AFRPIPLESATLLPPVVPPKIVCVGRNYREHAAELGNEVPKEPLIFLKAPSSLLAHKQ
                     EIRLPAISQRVDFEGELAIVIGKR"
BASE COUNT          864 a         1150 c         1106 g          876 t
ORIGIN      
        1 gtttctcttg aggtgtacta ttcacggcga cttcacctta catctctgga ggccgctgtg
       61 ttgaagcctt tgctcgtttc ttcagcgctg ctgctggttg ctctcttttc tgccgctcag
      121 gaccagccgt catcatcgca acaaccgtct tcttctgaat ctatccctca gacgaatcca
      181 gtaaaaccta caccgcaatc gcaggcccgc gccaaacaga tttatggcta cgattgcgcc
      241 atgtgtcatg gcgacaaggg cgatggcaaa ggcgacgttg ccaacgacat gaaactgacg
      301 ttgcgtgatt acaccaaacc cgacgccctc aaagacctga ccgacgccca gatgtacgac
      361 atcatcaaga atggcaaagg ccagatgcct ggcgaaggcc cccgggccaa acccgacgaa
      421 atctggaacc tgattatcta tctccgctct atgtcaaaga agactgcata ggcagacaag
      481 aagaccagtc aaaccattct gaatttcagc gagattggcc caatccggta tcgttcatgg
      541 gaaggacctc tcactgacac acatctggga caggaatctg caataagatt gccacacaca
      601 gcgctgaaat atcctgttat tacgccttgg cccgtaactc aggtatttgc tgagaaacca
      661 agggacgttg aggaggaggt ggagctgcgg atgccgtctt gttcggctga gaggccggta
      721 cctgcgctcc tactggtgtt cccataggcc gcaaatgcat ggccggttga tgtaccgcgg
      781 gctggtgcat gctcacgata atcgctttgg ctgaagcctt tccggcgcaa cggtacgagt
      841 gtggcgtact cgcgtcaaag tacacgccgt cgccagcctc aagcacgtgg actttttctc
      901 cgtgccggat ttccagatcg ccttcgagca cataaagaaa ttcgtatccc ggatgcacgt
      961 gtgactggat tggcactggc ttctttaccg gaatgaattc tgcatagtat ggatccagtt
     1021 gccggtctgg caccataaag cccatgcttt caaagtagta ggtgggatcg tctacgccgg
     1081 tctgtggaag acggacgcgc tcgccttttt tatgaatgcg gaagaccgtg tgcggttccg
     1141 tttcaaagaa ataggagagg tccttcccga agaccatcgc aatgcgggcc agattgcgca
     1201 gcgtgggcac gacgcggccg gtttcaagct gcgacaaaaa actggcggaa agtccggtgt
     1261 gcctgcccag ttctaccaat cccatggatt tcttgagccg cagtctcttg atgcgttcgc
     1321 caatatgttt ttccgcaatg aacgattcag cgctacctgg gtcaatggat ttctcaagtt
     1381 ccggcttttt gtttttcatg gcgcactctc ccggtcctat tccaatggat gccataaaac
     1441 ccgcatttat gttgcctgta ttaaaaactg tttccgttta atacacaact agtatttttg
     1501 tcaactttat acagatgtgt atgttagggt gaacaccttg cctgcttgtc cctgaaatgt
     1561 catttatttg tcatttatga gagttcttat cagcggcgct tccggattga ttggctcatc
     1621 gcttgcccgg gagctggcgt acagaggcgc tggcgtcttc cggcttgttc ggcggcgacc
     1681 ggaagatgcg cgggaagtgg cgttgccgcc tcagggccat cctgcccaat tcgaagattt
     1741 cacggcggtc atccacctgg cgggtgcgaa tatagctgcc catcgctgga cggaggctta
     1801 caaacgggag attgccgaga gccgcatcca gcccacggca aagctggcac acgtgctggc
     1861 cgggctgaaa cgcccgcccc aggtattcct ttgcgcttct gccattggaa tctatgggga
     1921 ccgaggcgag gaagcgctgg cggaggattc tcctgccggg accggatttc ttgccgaaac
     1981 ctgccgcgca tgggaagccg cagcgcagcc ggcgcgcgat gccggaatcc ggacggcaca
     2041 catgcgcttt ggcgtggtgc ttgcccggaa aggtggcgcg ctcgacaaaa tcctgcccgt
     2101 ttttcagctt ggggcaggcg gaagactcgg gagcggaaaa cagtggatga gctggatttc
     2161 tttgcaggac gcaatcagcg caattgcttt tctcatggag aaggaaacca tctatggccc
     2221 tgtcaacgtg gttgcgcctg cgccggtgac gaatgccgaa tttacccagt tgctggcgcg
     2281 gatgctgcac cggcctgcaa tttttcctgt tccggcattt gcgctgcggg ttgcgctggg
     2341 gcagatggcc gaggaagggc tactggccag cgcccgcgtc attccgcaga aacttcagca
     2401 ggccgggttt cgcttcgagc atccacaatt ggctgacgcg ctctctgcta cgctgaaaag
     2461 ggaatgagac aaaaagcgag atcatctttt atcggaattt ctcttcttgc cttgttccct
     2521 ctgcttattt cctgcggcct tgaatctccg ccccgcgcgc ccagcctgta cctgccgcag
     2581 ccccccacag atctgaccgc ctatcgcagc ggcaacgatg tctatctgca ttggacgatg
     2641 ccaaaacgct cgaccgaccg cgtcccactc cagggcggcc agcaggtcat gatctgccgc
     2701 agtttcgctg gcgatccatg cgagacggct ggcaaagcaa gatacgctcc gggaacggct
     2761 gcagatttca ccgaccatct cccggcgggc cttgtttccg gcgcacctca actgctgaca
     2821 tacaccgtcg agatcacaaa taaaaaaggc catgccgcag gcccgtccaa cccggcctat
     2881 acggcttcgg gaacggcccc ggcagcactc actgatctct ccgcggaagt ccatgccgaa
     2941 ggcgtactgc ttcgctggca gccagcgcag gggccgggaa atcttgtccg gctgaatcgc
     3001 attctgacca atccgccgaa gcgctcttct tcaccgatgg caggagcgcc agcacccgcg
     3061 gaacaaaaac tcgaaaccga ttatgtttca ggacacgacc ccggcgccac gctcgataaa
     3121 gatgcggcac tcgaccatat ctaccgctac acggcacagc gcattgcgtt ctttgaattc
     3181 ggcggccaca aggtggaagt ggcgagcact ccgagcgaaa cgattgtggt caacgcgcgc
     3241 gatgtctttc cacccgctgt gccttcagga ctggttgcgg tcgccaatcc ggaagggcac
     3301 tcgattgatc tttcgtggac accggacgca gaaaatgacc ttgccggata catcgtctat
     3361 cgccgagaag ccgggtcgag tgcaggcccg gtgcgcatct cgccgccgga gccggttgca
     3421 ggccctgcat tttctgacac gacagcaaaa ccgggcgtgc gctatgccta ttctgtcagc
     3481 gcagtggata aagaccacaa tgagagtgcc aggtcggcag aggtggaaga agctttgcca
     3541 aaattctgaa ggccggtgcc tagtcctgtc gagcgattgt tgcgtgagag ggcgtgcgcc
     3601 gcaatatgat gagagccgat gagatactgc cgtttccaaa ccaaagctgg cccacaatac
     3661 ggagaagtcg cagaccgcaa cggaacgctc tggattgagc ggctgctgcc tccgtttgaa
     3721 gaagacccct ggacgaagtt ggacgagaca gcattcaggc ccattccgct ggaaagcgcg
     3781 acactcctcc cgcccgttgt tccaccaaaa attgtctgcg tgggcagaaa ctaccgcgag
     3841 cacgctgctg agttgggcaa tgaagtcccg aaagagccgc tgatttttct caaagcgccg
     3901 tcttctctcc ttgcgcacaa gcaggagatc cgccttccgg caatctcgca gcgtgtggac
     3961 tttgaaggtg aactcgccat cgtcatcgga aagcgc
//
LOCUS       DASRBV010000273        18801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_271921, whole genome shotgun sequence.
ACCESSION   DASRBV010000273 DASRBV010000000
VERSION     DASRBV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 18801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18801
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271921"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFA02_10005"
     CDS             complement(<1..1055)
                     /locus_tag="VFA02_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU10224.1"
                     /translation="MKVCMVTYSFYETDNRVMRYAETLARRGDSVDVLSLCNPGQERT
                     THLNGVNIFRIQARTRNERGKFSYLARILRFFMRAAAWLTVKHAKQKYDVIHVHSVPD
                     FLVFAALLPKWMGAKLVLDIHDILPELYASKFGLSQSSALFRVLLLVERISTAFADQV
                     IIANHLWRDRIVARSVPAAKCIALMNYPDQSIFFPQGPTRKDGKFILMYPGTLQTFQR
                     LDIAVRAVQIIARELPNVELHLYGEGEAKSSLIGLTRQLGLENHVFFHPLRPIREIGS
                     IMAQADIGIVPKDAQSFGNEAQSTKILEFMSVGVPVVVADTKIERYYFDDSLLEFFRS
                     GDEHDFARAVLRIATDK"
     gene            complement(1052..1714)
                     /locus_tag="VFA02_10010"
     CDS             complement(1052..1714)
                     /locus_tag="VFA02_10010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10225.1"
                     /translation="MNASALITVPARAFHAVLKRWGASATKQKIWNAEYESGKWRDID
                     PQDSATIQRDLVYAVLEKWADRGDMLDLGCGTGSTAVQIDDVYRSYLGIDISPVAIEW
                     ARKHIAQNPAKALKTSFAVAPIEAFSTQRTFDVVLYRECLYYFALGQIKSILSGHSRF
                     LSDRGVFVVRLHDRNRYEKIVSHITQTYDVLEKVAPAGDPTIVLVFRPCPSRLVSGKA
                     TL"
     gene            complement(1711..3063)
                     /locus_tag="VFA02_10015"
     CDS             complement(1711..3063)
                     /locus_tag="VFA02_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10226.1"
                     /translation="MNPNHYAWVTDNMTFVSPLGLGVTLILCLALLFLPRRYAIVPVL
                     ALTCFMTMGQRVVLGGLNFTMIRILLLFGWARLAFRGEFRRIQWNAIDFCLIAWAISG
                     FVAHSILEPGDAAYRLGVAYNCVGTYFLMRYLLRERDDVVTVMRLLAWFVIPLSMLML
                     WEHFSGHNIFAFLGGVPAISEIREGKIRSQGPFGHSILGGTFGATVFPFFYALRFHSG
                     TDRVLGYMGMLASLIIVFSSGSSGPAFALIFSIIAFCLWPVRQHMRALRWGLLALAVV
                     LHLVMKAPVWFLIGRITVFQASTGFHRAMLIDAAIRNFSEWWLVGTPSTAHWGYELMD
                     VTNYYIRQGVEGGFLTLVLFMAVISYAFGAVGRARKAAAKQPRWEQMMIWALGAALLA
                     HAVSFISVSYFDQNFVSWYLLLAMISHISTLKPAEQQVIAPAKVRRSWRDMQWEGVAR
                     "
     gene            complement(3060..4052)
                     /locus_tag="VFA02_10020"
     CDS             complement(3060..4052)
                     /locus_tag="VFA02_10020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU10227.1"
                     /translation="MKSGMELSIIIVNWKSADYLRKCLKSVYAGISSLRCEIIVIDNA
                     SGDGCGEMLALEFPDVIFIASHKNLGFAGANNLAFERSVGRYVLFLNPDTELLPGALR
                     ELMDALDASPAAALIGAQLLNSDLSLQDSCIQRFPTILNQLLDFEFLRKKLPPSDFFA
                     TRPFHGQQNSIAEVDCVSGACMLVKRELFSAVGMFSTRYFMYSEDVDLCYKIKLAGGK
                     VYYAPRARVVHHGGRSSTLEPQSHFATVVMRESRWRFMTAWYGSAYAVAYRMTTAFGA
                     VARIAMLGAAIAITRNRERRLRFSGAVQKWAKVLRWSLGMESWAKQLEVKVQGA"
     gene            complement(4049..5956)
                     /gene="asnB"
                     /locus_tag="VFA02_10025"
     CDS             complement(4049..5956)
                     /gene="asnB"
                     /locus_tag="VFA02_10025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZU10228.1"
                     /translation="MCGICGVMEMGGRPVLKTDVAAMMSTLAHRGPDGAGSYVQGEVG
                     LGHRRLSIIDLSGGAQPISNEDGSLHIVFNGEIYNYIELRDELLTHGHVFVTNSDTEV
                     IIHAYEQWGADCLLRFNGIFAFALWDQNKRELFLARDHLGVKPLYFTQRGNRFSFASE
                     VKALLSDSECERDVDLTALAQLFTLRYVPSPRTLFQGISKLPPAHWMRVSHSGIEIKH
                     YWTRVPEINTQTSVETLVEQYQSLVEDAVRLQMRSDVPVGLFLSSGVDSGALLALMSR
                     LTNHPVKTFTIGFDGGERTNETHEARSMAGMFGSEHSEMIVSARDYEDYYERYLWDIE
                     EPVGNETAAAFYFVSLIAGRSVKVALTGQGADEPWGGYHRYIGVQLSKTYSRLPRGLT
                     DGIVRPLVCSFIKQEKLRRGVISLTEPDVLKRFINIYSFYDSRMKHGLFQPWLREAIG
                     TEGSQACGPLASLQAPVSHLDPVSQMMYIDTRANLPDDLLMVGDKTSMANSLEARVPF
                     LDPRVVEFVESLPPNLKLHRFVRKYLHKKALEKYLPREIVYRKKKGFANPVNQWLRSS
                     MKKYIGDCLLSGSSAVNQYFNRTYVQQMLADHESGRQEHLRHIYLLISFELWHRTFMR
                     SGRPASCEVAS"
     gene            complement(5956..6846)
                     /locus_tag="VFA02_10030"
     CDS             complement(5956..6846)
                     /locus_tag="VFA02_10030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZU10229.1"
                     /translation="MDNKRLGSYVIVSPVKDEEKFVRETLESVTRQTILPLRWIIVDD
                     GSCDQTLSIIEEYTSRFHWITVVQTQGGARQPGSAVIRAFNHGMTFLHDTEYSFIVKL
                     DCDLRLPADYFEQMLSRFARDPKLGIASGVYQETKNGRTHLVRMPPYHAAGASKMMRA
                     ACFMEIGGFVPERGWDTLDEIRAQTRGWRTCHFDDLVLDHLKPEGTGIGYLRTNRMHG
                     EIFYRTGGGALFFLGKAAARALHGRPFVMGGMAMLYGYLSARLSGRKRLVSSEEARHY
                     RALLNSRIFDGMRRGLGRAF"
     gene            complement(6839..7567)
                     /locus_tag="VFA02_10035"
     CDS             complement(6839..7567)
                     /locus_tag="VFA02_10035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU10230.1"
                     /translation="MAESKRHPYYELRTPVKNFEQQMSWLARNGYRSLCMSDIAPEML
                     CGSAAKHVVITFDDGFRDFYTSAFPVLRQYGLTATVFLPTAYIGTQRMQFLGMDCMIW
                     REVEELDKAGIEFGSHSVSHPQLYKLPQSSLEDEIAGSKQHIEEHLQKPVQSFAHPYA
                     FPADSTYSERLRILLVKHGYTSGVCTTLGLANASSDRFFLRRIPVNGFDDLALLQAKL
                     EGAYDWLGTVQSMKKSIVRMVKHG"
     gene            complement(7694..8671)
                     /locus_tag="VFA02_10040"
     CDS             complement(7694..8671)
                     /locus_tag="VFA02_10040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU10231.1"
                     /translation="MQGPLVSVIIPTYNRAHLIEGAIASVLRQEYRPLEIIVVDDGST
                     DNTSDLMARKYPRLLYVRQANSGPSVARNQGVRLSSGEIIMFLDSDDEWLPGKVKRQV
                     DLLRDAGPSACCCVTNAILRETNGKNQLSFDVAEMYPLLANGLWTNPAEVLASRFLFF
                     NQVVAVRRDAIVQCGGFNERLTLLEDWDLALRLAVLGPWAYTTEPFAVWNPGSNGSLV
                     ARAARDRISLRESSLTVHQSALAREGAASRTQTADLLRRKIRTTQRELLGLHLAQDKS
                     IWKRAAGNSITLAERCRTALSKRLRRWPEMQVQALTSGPAVLEYEKATA"
     gene            complement(8671..9759)
                     /locus_tag="VFA02_10045"
     CDS             complement(8671..9759)
                     /locus_tag="VFA02_10045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZU10232.1"
                     /translation="MTTTAAGLSFASIKRRTEDQDSFLQKLAHEWRRAERSRRPLLLV
                     LFEQRNGTTSGELEAVPEILAPATRETDIWGWFEQKTVLGAIFGELGTAPVEEARRVI
                     LAKLRAQLQQSRIQNVSLTAYVLPAEFASGSGESADDISDILCSLEAREKRLQQVLKR
                     VADIAGSALLLLLLSPLFLLIAIAVKCTSRGPAFFRQTRVGRHGKHFTFLKFRSMKAG
                     NDPSIHEAYCRSLIEGRAEQQKDKNGNKVFKITGDPRITPIGKILRKTSLDELPQLWN
                     VLCGEMSLVGPRPPIPYEVSCYDYWHKRRVLEVKPGMTGLWQVRGRNRTTFDEMVRLD
                     LRYARKWSLWLDFKILLETPFAVLKGAH"
     gene            complement(9995..10363)
                     /locus_tag="VFA02_10050"
     CDS             complement(9995..10363)
                     /locus_tag="VFA02_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10233.1"
                     /translation="MRRSSRSLCLFCISLSLTACGTSRPSPSGASTSTPPTTGASQHE
                     VDLQWTPSPSDVAGYRVYRGTQHNGPYSLLTSTLVTGTSYADLQVSAGETAYYVVTAV
                     NADGVESDYSPEAQATVPAP"
     gene            complement(10481..11689)
                     /locus_tag="VFA02_10055"
     CDS             complement(10481..11689)
                     /locus_tag="VFA02_10055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZU10234.1"
                     /translation="MKTIVPFLDLVTPHLELEEELVQVFRKSLHTAGFIGGGPVRSFE
                     EHFSAYCEAADCVGVASGTDALLLAFLAGGIRPGETVLTVANTFIATAEAITQAGAVP
                     EFIDIEERTCTMDVEKLRSYLENCSLDPVTGKRLSQRTGNPITAVVPVHLYGQMAEMD
                     AIMEIAEEFGLQVYEDACQAHGAKYFSQREGRWLSAGSIGRAAAFSFYPGKNLGACGE
                     AGAVTTNDPEIARKVRVLRDHGQPRKYFHEAIGYNGRLDAIQAGFLDVKLKYLPRWTD
                     QRRAVARAYDRMLSDVDGIVLPHERENSQAVYHLYVIRVEDRDAIQAKLAERGIGTGI
                     HYPVPLHLQPAYDFLGYRAGDLPVTEELANTALSLPMYPGLSLEDQEYVIEALSSAVG
                     ELTLAIGETR"
     gene            complement(11686..12183)
                     /locus_tag="VFA02_10060"
     CDS             complement(11686..12183)
                     /locus_tag="VFA02_10060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU10235.1"
                     /translation="MNDFVCISPDVKLGKDVKLSRFINLYGCEIGDNTKIGAFVEVQK
                     NAKVGRNCKISSHTFVCEGVVIEDNVFVGHNVTFINDSYPRATTADGQLQTEKDWVVE
                     KTLVKKGASIGSGVTVLSRVIIGENAIVGAGSVVTRNVPDNTIVAGVPARLMRSLDEE
                     KALMK"
     gene            complement(12180..13322)
                     /locus_tag="VFA02_10065"
     CDS             complement(12180..13322)
                     /locus_tag="VFA02_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU10236.1"
                     /translation="MGDIPVTVDVRPVIADTSFDVKMLGHLQHMPMPWTEIRPKPALR
                     SDVLRVGVVGYGYWGPNIVRNFSAQEHSQVTMVCDRRPEALARIMKSHPDVEVSTEAM
                     EMVRSPHIDVIAVVTPVWTHYELAKAALLHGKHVFVEKPFTSNSAQAEELIELAERKG
                     LKIMVDHTFLFTPAVQKIKQLIEARELGDIYYYDAMRVNLGLFQHDVNVIWDLAPHDF
                     AIMDYLLPERAEAVVATGQKHVNGVEDVAFVTIYYPNKLIAHVNVNWLSPVKVRTTLV
                     GGDKKMLIWNDLESDEKIKVYDRGVKHTRTGEGIYDLLVSYRSGDMWAPQLENAEALK
                     LEIAHFNECIREDKRPINDGISGLRIVKMLQAADESLKNRGTQVLI"
     gene            13651..14823
                     /gene="wecB"
                     /locus_tag="VFA02_10070"
     CDS             13651..14823
                     /gene="wecB"
                     /locus_tag="VFA02_10070"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZU10237.1"
                     /translation="MKVSVILGTRPEAIKLAPVILAFRASREFECHVCVTAQHRQMLD
                     QVLEVFRIVPDADLDLMTEGQTLSGLTARALTALDVYLAREKPDLVLVQGDTTTVFTA
                     SLAAFYHGIPIGHVEAGLRTWNLRSPWPEEANRVLTSRLAGLHFAPTESARQNLLREG
                     IPDGDIFVTGNTVIDALYLAADRARGSSPEIPGLPFELMMDGTPLVLITGHRRESFGE
                     KLEAICTAILDLAETFPEAHFVYPVHLNPQVRNTVMRMLSASSSGNIHLIAPLSYLPF
                     VALMQRATLLITDSGGIQEEAPALGKPVLVTRDTTERPEALAGGTVKLVGADRAGIVR
                     EASRLLGDAAYLHEMSRICLPYGDGKATERILAAIATRFDTAPVQQEELLPVAVGR"
     gene            14823..16181
                     /locus_tag="VFA02_10075"
     CDS             14823..16181
                     /locus_tag="VFA02_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10238.1"
                     /translation="MTLRKRILMISPLFPPTADSEAFCAGKMAQALRNTGCDVEVIFC
                     SNYPGAVRRDNSQLWKDFSSFSHNVPLSFGSWTRSVPSALRFGTVFFSRWVRDAVQLA
                     GQLHAANPFDFVQSRSLPMIAHVAGHHISKELGIPWNANINDPWDFHLFPDAMRVKWS
                     NRCVHAIRAAAHFQRIGVVSSYWMRKTFRTADLVTYCSARLEKYHLALAGVPHRSAIV
                     PHIGWSVPCDGGQGLHLVHAGKLGANEFTGRSTRTLLHGIAAFVQKRQPEHTEFLLTL
                     VGPEDPETAGLVRALGLEKFVRSIGPVSYEESLRWIASASVCVLVEARMDEGIFFPSK
                     CADYIAAGKPILAFSPQTGVIADMASLGTGIVQIREGDSDGVAAALERFCLAQQKGTL
                     EEFAPDAALRSMCSPEEVTRAFLETAGPRLSIGREKQAARFPLWEKTFTSPQPDPGSA
                     QK"
     gene            16178..17557
                     /locus_tag="VFA02_10080"
     CDS             16178..17557
                     /locus_tag="VFA02_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU10239.1"
                     /translation="MTRFLHLLPRPVQQWMHNNPMGRRLAHGAFWTLLGTIASRVLAI
                     PVSVVLARWMGPSHYGELGVINNSVDLFIVFAGFGLGLTATKHVAELRTTDPERAGRV
                     LALSTVTAGFTATITAVVLYIAAPYLAAHTLNAPQLTGPLRIGSLILLLAAINGAQQG
                     ALYGFEAFRTTAKIQAIVGLLNLPFSLIGYWLGGLNGVLWGMVATRGADWLLRRLALL
                     DESRKSGVPLGLKGWTQELHVLWKYSVPALLSGVMVAPVNWVCAAMLVNQPKGYAQMG
                     IYNAANQWYGALLFLPTVLGSGLLPLLSERMGNKDSHSSGKVLLFMLRLNAVIVLPAA
                     FLGSALSPWIMRLYGPAYAHGWSTLVAVLFTAAVFAVLIPVGDVIAASGRMWTGTVMN
                     AGWAAVFIAGTAVLVHRGAFGLGLARLIAYIAHAIWTIAFAWILITRAKASQQAISSA
                     AITAGSEAA"
     gene            17687..>18801
                     /locus_tag="VFA02_10085"
     CDS             17687..>18801
                     /locus_tag="VFA02_10085"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HZU10240.1"
                     /translation="MKKTLLFAIALCLMVMRPAQAQNAGWMDPAWLYRNPVTINNPSG
                     TALSQFQVNIHLGSETNFGAAKSGGADLRVTASDGVTQLPFWIESWNASSSQASIWVK
                     VPSIPTTGTTLFIYYGNPNAASASNGNNVFEFFDDFETPYGTQTGYFAQSSPTTVMVQ
                     DQTWEANPPHTLSVVQANQNGYTYSGYYGTYQCDGIGLAYSNDLTHWTKYTGNPLISG
                     GRWPTVQLVNGVYYMLYEKNFCTSSSYIELATSTDGLHFTDVKPIVKAQSGLRNQNPN
                     LFFNPNDNLWYLYYYHGDDSSNFNIYVRSAFSITALDTASTTTVLSSKAKMAAPSMFY
                     YNGKYYLSTEGTDPTQQWVTIVYSSSSPKSGFTLVPG"
BASE COUNT         4363 a         4971 c         5174 g         4293 t
ORIGIN      
        1 tccttatcag ttgcgatgcg aaggacggca cgggcaaaat cgtgctcatc tccgctgcgg
       61 aagaactcca gcaatgaatc atcaaagtaa taacgttcaa tctttgtgtc ggccacaaca
      121 acggggacac caacagacat aaactcgaga atcttggtgc tttgggcttc gttgccgaag
      181 gactgtgcat ccttcggtac gattccgatg tctgcctgcg ccatgatgga accgatctcg
      241 cggataggac gaagcggatg gaaaaagaca tggttttcca gtccgagttg acgggtcaat
      301 ccgatcaggc tgctttttgc ctcgccttct ccatacaggt ggagttcgac atttggaagt
      361 tcgcgcgcaa tgatctgcac cgcgcgcacg gcgatgtcga gtctttgaaa tgtctgcaga
      421 gtgcctggat acatcaggat gaacttgcca tctttgcgag tggggccctg tgggaaaaag
      481 atggattggt cgggatagtt catcaatgcg atgcactttg cggcaggcac agagcgcgca
      541 acgatacggt cacgccacag atgattggca atgatgacct gatcggcaaa tgcagttgag
      601 atacgctcta caagaagcag aacacgaaag agggcactgc tctgcgaaag gccgaatttg
      661 ctggcataga gttccggcag gatatcgtga atatcgagga caagttttgc tcccatccat
      721 ttggggagca gagcagcgaa gacaagaaaa tcgggaacag agtggacgtg gatgacgtcg
      781 tacttttgtt ttgcgtgttt gaccgtaagc catgcagcag cacgcataaa aaacctgaga
      841 atacgcgcaa ggtaggagaa ctttccgcgc tcattgcgcg tgcgtgcctg aatgcggaag
      901 atgtttacgc cattcagatg cgttgttcgt tcctggccag gattgcagag agagagcacg
      961 tctacggaat caccgcggcg ggcgagtgtt tcggcgtagc gcataacacg attgtcggtc
     1021 tcatagaacg aatatgtgac catgcagact ttcatagagt ggcctttcct gaaacaagac
     1081 gggaggggca cggcctgaac acaagtacga tggttggatc gccggcgggt gcaaccttct
     1141 cgagaacgtc gtatgtttgc gtgatgtggg aaacgatctt ttcgtaacgg ttgcggtcat
     1201 gcaaccggac aacaaaaact ccccgatcgc tgagaaaccg ggaatgcccg ctgagaattg
     1261 atttgatctg accgagtgca aagtaataca ggcactcgcg ataaagcacc acatcgaatg
     1321 tgcgctgtgt tgagaaggcc tcaattggag cgactgcgaa gctggtcttt aacgctttcg
     1381 ccggattttg cgcgatgtgt tttcttgccc attcgatagc gacgggtgag atatctattc
     1441 ctaaataaga gcggtataca tcgtcaattt gtacagctgt cgaacctgtt ccgcagccca
     1501 gatccagcat gtcaccgcgg tccgcccatt tttccaggac ggcatagacc agatcgcgct
     1561 ggatcgtcgc cgaatcctgg ggatcaatgt cccgccactt tccggattca tattcggcgt
     1621 tccatatttt ttgttttgtc gcagatgcac cccagcgttt gagaacagcg tgaaaggcgc
     1681 gtgctggaac agtaataaga gcgctggcat tcaccgggca actccttccc actgcatatc
     1741 gcgccaggag cggcgtactt ttgcgggcgc aatcacctgc tgttctgccg gtttgagcgt
     1801 ggaaatgtgt gaaatcatgg ccagcagcag gtaccaggaa acgaagttct gatcaaaata
     1861 tgagacagaa ataaaggaga cggcatgcgc caggagtgcg gcgccaagtg cccatatcat
     1921 catctgctcc caccgaggtt gctttgcagc agctttgcgg gcgcgcccta ctgctccaaa
     1981 ggcatatgaa attacagcca taaacaagac cagagtcagg aagccgccct cgacaccctg
     2041 gcgaatgtag taattggtga cgtccatcaa ctcgtaaccc cagtgtgcgg tagatggtgt
     2101 gccgacaagc caccactcag aaaaattgcg gatggcggca tcaatcagca tggcacggtg
     2161 gaagcctgtc gatgcctgga agacagtaat gcgtcctata aggaaccata ccggggcctt
     2221 catgaccaga tgaaggacta ctgctaacgc gagaagtccc caacgcaaag cgcgcatgtg
     2281 ctggcgtacc ggccacaggc agaaggcgat gatgctgaag ataagtgcaa aggcggggcc
     2341 gctcgatccg gaactgaaga cgatgatgag cgaagctaac attcccatat agcccagaac
     2401 tctgtcagtc ccgctgtgaa agcgcagagc ataaaagaaa ggaaaaacag tcgcgccaaa
     2461 tgtgccgccc agaatcgagt gcccaaatgg cccctgtgaa cggattttac cttcgcgtat
     2521 ttcgctgatg gctggaactc cgccgagaaa ggcgaagatg ttgtgtccgc tgaagtgctc
     2581 ccacagcata agcatggaaa gcggaatgac aaaccaggcg agcaggcgca tgacagtgac
     2641 gacgtcatcc cgctcacgga gcagataacg cataaggaaa tatgtgccca cacagttata
     2701 ggccacacca agccggtatg ctgcgtcgcc cggttcaagg atggaatgcg caacaaaacc
     2761 agatatggcc catgcgatga ggcagaagtc tatggcgttc cactgaatcc tgcggaactc
     2821 accgcgaaat gcaagccgtg cccatccaaa gagaagcagg atgcgaatca ttgtgaagtt
     2881 caggcctccg agtacaacac gttggcccat cgtcataaag caggtaagcg ccagcaccgg
     2941 gacaatggca taacgacgcg gcagaaacag cagggcgaga cacaatatca gcgtgacgcc
     3001 cagtcccaga ggactgacaa acgtcatgtt gtctgtgacc catgcatagt ggttcggatt
     3061 catgcgcctt gtacctttac ttccagttgc tttgcccagg attccattcc gagcgaccag
     3121 cgcaacactt tggcccactt ctgcacagcg cccgagaagc gcaaacggcg ttctctgttg
     3181 cgggtaattg ctatggctgc acctaacatt gcaattcttg ccactgcgcc aaaggcagtg
     3241 gtcatgcggt atgcgacagc ataggcagag ccataccagg cggtcataaa tcgccagcgt
     3301 gattcgcgca tgaccacagt ggcaaagtgg ctttgtggct ccagggtgct gctgcggcct
     3361 ccgtgatgca caacgcgcgc acgcggagcg taatagacct ttcctccggc aagcttgatt
     3421 ttgtagcaga gatcaacgtc ttctgagtac ataaagtaac gggtactgaa catgcccacg
     3481 gcagagaaca gttcgcgttt taccagcatg catgccccag agacacaatc cacttctgcg
     3541 atgctgtttt gctggccgtg gaaaggccgt gtggcaaaga aatctgatgg cggcaatttc
     3601 tttcgcagaa actcaaaatc gagcaattga ttgagtatgg tgggaaaccg ctgaatgcaa
     3661 ctgtcctgca ggctgaggtc agaattcaaa agctgtgctc cgatcagggc cgcagcaggt
     3721 gacgcatcga gcgcgtccat caactcgcga agcgcgccgg gcagcagttc cgtgtccgga
     3781 ttaaggaaga ggacgtaacg cccgacggaa cgttcaaagg ccaggttgtt cgccccggcg
     3841 aagccaagat ttttgtggct ggcgatgaaa atgacatccg gaaactccaa agccagcatt
     3901 tcgccgcaac cgtctccgga ggcgttgtca atcacgatga tttcgcagcg cagagaagag
     3961 atgccggcat agacactctt caggcatttc cgaagatagt ccgcggattt ccagttgacg
     4021 atgatgatgg aaagctccat gccggatttc acgacgccac ctcacagctg gctggacggc
     4081 cagagcgcat aaaggtacgg tgccaaagct caaatgaaat caagaggtaa atgtgccgca
     4141 ggtgttcctg gcgtcctgat tcgtggtcgg cgagcatctg ctggacataa gtacggttga
     4201 aatactgatt tacggcagaa gacccagaga gcaggcaatc accgatgtac tttttcatgc
     4261 tgctgcgcag ccattgattg accggatttg cgaagccctt cttcttgcgg tacacgattt
     4321 cgcgcggaag atatttctcc aaagcttttt tgtgcaggta tttgcgcaca aagcggtgca
     4381 gcttcaggtt tggcggcagg gactcaacaa actcaaccac tctggggtcg agaaatggca
     4441 cgcgcgcttc aagcgagttt gccatggaag tcttgtctcc caccatcagc aggtcgtcag
     4501 gcaaattggc gcgtgtatct atgtacatca tctgggaaac gggatcgaga tgagacacgg
     4561 gtgcctgcaa agaagcgagt ggtccacacg cctgactacc ctctgtgccg atggcctcgc
     4621 gaagccaggg ctgaaacagg ccgtgtttca tgcgtgaatc atagaaagag taaatattga
     4681 tgaagcgctt gaggacatcc ggctcggtaa gggagatgac gccacggcgg agcttctcct
     4741 gcttgataaa agagcagaca agcgggcgga cgatgccgtc tgtcagtccg cgcggaaggc
     4801 ggctgtatgt tttagaaagc tgcacgccga tgtagcggtg gtagcctccc cacggctcat
     4861 cggcaccttg tccggtgagt gccactttta cggagcggcc agcaatcagg ctgacgaagt
     4921 agaatgccgc tgccgtttca ttcccaaccg gctcttcgat gtcccacaaa tagcgttcgt
     4981 agtaatcttc atagtcgcgc gcgctgacaa tcatctcgga atgttctgag ccgaacattc
     5041 cggccatact gcgggcctcg tgggtttcgt tggtgcgctc gcctccatca aacccgatcg
     5101 tgaatgtctt gaccgggtgg tttgtcagcc tgctcataag ggccaacaga gcacctgaat
     5161 ccacgcctga gctgaggaaa agccctacag gtacatcgct gcgcatttgc aggcgcacgg
     5221 cgtcttcaac cagcgactgg tattgttcca caagcgtctc gacggacgtc tgcgtattga
     5281 tctccggaac tcgcgtccag tagtgcttta tctctatgcc tgagtgagag acacgcatcc
     5341 agtgcgcggg cggcagtttg ctgatgccct gaaagagcgt cctgggcgat gggacatacc
     5401 ggagcgtgaa gagctgcgcc agagcagtaa gatctacatc ccgctcgcat tcagagtcag
     5461 aaagaagggc cttgacctct gaagcaaaag agaagcggtt tccgcgctgt gtgaagtaca
     5521 gaggttttac acctaaatga tcgcgagcaa gaaacagttc acgcttgttc tggtcccaca
     5581 gggcgaaggc aaaaattccg ttgaagcgca gcaggcaatc tgcgccccat tgttcatatg
     5641 catgaatgat gacttctgta tctgaattcg ttacaaaaac gtggccatgt gtaagaagct
     5701 catctctaag ctcaatatag ttgtagatct ctccattgaa gacgatatgc aaggagccat
     5761 cttcattgga aatcggctgc gcgccaccgc ttaaatcaat aatgctcagg cgacggtgcc
     5821 ccaagccaac ttcgccctga acataactgc cggcgccatc agggccgcga tgcgcaaggg
     5881 tggacatcat cgccgcaacg tctgttttca ggacgggcct tccgcccatt tccattactc
     5941 cgcaaatgcc acacatcaga aagccctccc taatccacgc ctcatgccat caaaaatgcg
     6001 gctgttcaac agcgcgcggt agtggcgcgc ctcttcagaa ctgacgagcc gttttctgcc
     6061 gctcaagcgc gcagagagat atccatagag catggccatg ccgcccatca caaacggtct
     6121 gccatgcagg gcgcgcgcag ccgctttacc gagaaagaaa agcgcgccgc ctccagtcct
     6181 gtaaaagatt tcgccgtgca tccgattcgt gcgcagatag ccgatgcctg ttccttcggg
     6241 cttgaggtgg tccagcacga gatcgtcaaa gtggcaggta cgccagccgc gagtttgagc
     6301 acggatttca tcgagggtgt cccaaccgcg ctcagggaca aagccgccaa tctccataaa
     6361 gcatgcagcg cgcatcatct tgctcgcgcc tgctgcgtgg tatggaggca ttcttaccag
     6421 atgagtgcgt ccgttcttcg tctcctgata tacacccgat gcaattccca gtttcggatc
     6481 gcgtgcaaag cgtgaaagca tttgttcgaa atagtcagcg gggaggcgca gatcgcagtc
     6541 cagctttacg atgaagctgt attctgtgtc gtgcagaaat gtcattccat gattgaatgc
     6601 gcgaattaca gcagaaccag gctggcgggc gccaccctga gtttgcacga ctgtgatcca
     6661 gtgaaagcgc gaagtgtatt cttcaatgat gctcagggtc tggtcgcacg agccgtcatc
     6721 tacgataatc cagcgcagcg gcagaatggt ttgccgtgtc actgactcaa gtgtttcccg
     6781 gacgaacttt tcctcgtcct ttacgggaga aacaattacg tatgagccaa gccgtttatt
     6841 atccatgctt caccatgcga acaatagatt tcttcatgga ctgcactgtg cccagccagt
     6901 catatgcgcc ttcgagttta gcttgtaata aagcaagatc atcaaagcca ttcacgggga
     6961 tgcgccggag aaagaagcgg tcagaagatg catttgcaag acccagggtt gtgcatacgc
     7021 cggacgtata gccatgcttt accagtaaaa tgcggaggcg ttccgaatac gtagaatctg
     7081 ccgggaacgc atagggatgg gcgaagcttt ggaccggctt ttgcagatgc tcttcaatgt
     7141 gctgcttgga gccggcgatt tcgtcttcga ggctgctttg cggcaactta taaagttgcg
     7201 gatgcgaaac ggaatgcgag ccaaattcga taccagcctt gtccagttcc tcaacttcgc
     7261 gccatatcat gcaatccatg ccaagaaatt gcatgcgttg tgtgccgatg tatgcagtgg
     7321 ggaggaagac cgttgcagtc aacccgtatt gccgcaggac cggaaacgca ctggtgtaga
     7381 aatcgcggaa accatcgtcg aaagtaatca caacatgctt agcagcggaa ccgcacaaca
     7441 tttccggtgc gatatcactc atgcaaagcg agcgataacc attccgggca agccagctca
     7501 tctgttgctc aaagtttttg acgggcgtgc gcagctcgta atacggatgg cgttttgact
     7561 ctgccatttc agagatgctg tgatacatca aaatgggtaa accgcggcgc ttcagccagg
     7621 gctgtgcgat atgtaccgtg attgttctgt ccagccgaaa catggttttc tctacttgct
     7681 catttcgcgc ccgtcatgcg gttgctttct catactcaag gacagcggga ccggatgtaa
     7741 gggcctgcac ctgcatctcc ggccaacggc gaagcctttt agaaagtgct gtcctgcacc
     7801 gttcggcaag agtgatgctg tttcccgctg cccgcttcca aatgctctta tcctgagcaa
     7861 gatgaaggcc aagtaactcc cgctgagtgg tcctgatttt gcggcgaagc aagtcagcgg
     7921 tctgtgtgcg cgacgcggcg ccttctctgg caagcgcgct ttgatggact gtcaacgatg
     7981 attcgcgtaa agagatgcgg tctctggcgg cacgggccac caggctcccg ttgctgccgg
     8041 ggttccatac agcgaaaggc tcggttgtgt atgcccacgg gccaagcaca gcaaggcgca
     8101 aggcaaggtc ccagtcttcc agcaaggtca ggcgttcatt gaatccgcca cattgaacaa
     8161 tcgcatcccg tctgacggca actacctgat tgaagaacag aaaccgcgag gccagcactt
     8221 cggctggatt tgtccaaagg ccatttgcca gcagcgggta catctcggcc acatcgaagg
     8281 aaagctgatt cttcccgttc gtttcgcgca agatcgcatt ggttacacag cagcacgccg
     8341 atgggcctgc atcccggagc aggtctacct gccgttttac ttttccgggg agccactcgt
     8401 catccgagtc aagaaacatg atgatctcgc ctgatgacag gcggacgccc tgatttcgcg
     8461 caacagaagg cccgctgttg gcctgccgta catacaagag gcgcggatac tttctggcca
     8521 tcaggtctga agtattgtca gtcgagccat catccacaac aatgatttcg agcggtctgt
     8581 actcctgccg caaaacgctt gcgattgcgc cttcaatcaa atgcgcgcgg ttgtatgtgg
     8641 gaatgatgac acttacgaga gggccctgca ttaatgcgcc cctttcagca cggcgaacgg
     8701 tgtttccaga aggattttga aatccagcca aagagaccat ttgcgtgcat agcgaaggtc
     8761 aagcctgacc atctcatcga aagttgtgcg gtttcgtccg cgtacctgcc acagcccggt
     8821 cattcccggc tttacttcaa gcacgcgccg tttgtgccag tagtcatagc atgagacttc
     8881 atacggaatc ggcggacggg ggccaaccag ggacatttct ccgcagagca cattccacaa
     8941 ttgcggcagt tcgtcgaggc tggtcttgcg aagaattttt ccgatgggtg taatgcgcgg
     9001 atcgccagtg attttgaaaa ctttgttgcc attcttatct ttctgctgtt cggccctgcc
     9061 ttcgattaag cttcggcaat acgcttcatg gatggatgga tcattgccgg ccttcatgga
     9121 gcggaatttg aggaaggtga agtgtttgcc atgccgtcca acccgggtct gccggaaaaa
     9181 ggcaggtccg cgcgaggtgc atttgacggc gatagcaatc agcagaaaga gcggtgagag
     9241 caaaagcagc aacaaggcag atcccgcaat atcggcaact cttttcagga cctgctgcaa
     9301 acgtttttca cgggcctcca gcgagcacag gatgtccgaa atgtcatcag cggactctcc
     9361 gcttccggag gcgaactcag caggcaatac atatgccgtc agggaaacat tctgaatcct
     9421 ggattgctgt aattgtgcgc gaagctttgc cagaattacc cggcgggcct cttctaccgg
     9481 agcagtgcct aattcgccga atatggcacc taaaaccgtt ttctgttcaa accatcccca
     9541 gatgtcggtt tcacgcgtag cgggagctaa gatttcaggg accgcttcca attcgcccga
     9601 tgtcgttcca ttccgctgtt caaacagcac caacagcaat gggcggcggg accgttctgc
     9661 gcgccgccac tcatgcgcca gcttttgcag gaaggagtcc tggtcttcag tacgcctttt
     9721 tatggaggca aatgatagcc ctgcagccgt tgttgtcacc ttgcgtctcc tcattacgca
     9781 atgcgaaggg ggcacactgc ggcaatcatg aggagagcaa ttactgttcc agaatcatgg
     9841 atttgaacat gggagaacac ggcaatttgc aggttttttc aacatgaatc cctcggaccc
     9901 ggacaaagcg tgcaatggta cgtgtaacgg gtcccatgga gcggaaaaga ttttctccgc
     9961 agcatgaagc ggttttcata tatccgttta tccgctatgg ggccgggacg gttgcctgcg
    10021 cttccggaga gtagtcgctt tccacgccat cggcattcac ggcagtcacg acgtaatatg
    10081 ctgtttcgcc agccgatacc tgaaggtctg catacgaagt acctgttacc agcgtggagg
    10141 taagcaaaga ataagggcca ttgtgctgag tgccgcgata cacccggtat ccggccacat
    10201 ctgaaggact gggtgtccat tgcaggtcaa cctcatgctg agaagcgcct gttgtgggcg
    10261 gagtagacgt agatgcgccg gaaggagagg gtctgctggt tccgcaggcg gtgagagata
    10321 acgaaatgca gaacaaacaa aggctgcgag agcttcgtcg caaggaaagc ctccgtagaa
    10381 ggaaaatggt cctgaatgaa tgtacagaaa agattactcc gatgactggc aggaaagcat
    10441 cttttccctg cgcggccatc ggagaatgaa aacggaaaag ttacctggtt tccccaatcg
    10501 caagggtaag ctctcccacg gctgaggaga gggcctcaat cacatactcc tggtcttcga
    10561 gtgagaggcc gggatacatc ggcagtgaaa gagcagtatt cgccaattct tctgttaccg
    10621 gaagatcgcc tgcccggtaa cccaggaaat cataggccgg ttgcagatgg aggggcacgg
    10681 gataatgaat acctgttccg ataccgcgtt ccgccagctt cgcctgaatg gcatcccggt
    10741 cttccacgcg gatgacataa aggtggtaga cagcctgaga gttttctctc tcatgtggaa
    10801 gcacaattcc gtccacatca gaaagcattc tgtcgtaggc gcgtgccaca gcgcggcgct
    10861 ggtccgtcca cctgggcaga tactttaatt tcacatcgag aaaacctgcc tgaatggcgt
    10921 cgagccgtcc gttatagcca atggcttcat ggaaatactt gcgcggttgt ccgtggtcgc
    10981 gcagcacgcg tactttgcgg gcgatttccg gatcattagt ggtaactgcg cctgcttcac
    11041 cgcaagcacc gaggttcttg cctggataaa agctgaaagc agcggccctg ccgatagatc
    11101 cggcagagag ccatctgcct tcacgctgag agaagtattt cgctccatgc gcctggcagg
    11161 catcttcata gacctgcaaa ccgaactctt ccgcgatttc catgatggcg tccatctctg
    11221 ccatctggcc gtaaagatgg accgggacca cggcggtgat gggatttccg gtgcgctgac
    11281 tgagccgctt gcctgtaacc gggtcaagcg agcaattttc cagataggag cgcagtttct
    11341 ccacatccat tgtgcaggtg cgttcttcaa tatcaataaa ttcaggaaca gcgcccgctt
    11401 gtgtaatggc ttcagccgta gcgatgaatg tatttgctac ggtgagcacc gtttcgccag
    11461 ggcgaatgcc gcccgccaga aatgcaagaa gcagggcgtc tgttccgctg gccaccccaa
    11521 cgcaatcggc ggcttcacaa taagcagaga agtgctcttc aaaactgcgc acaggcccgc
    11581 cgccgataaa cccagcggtg tggagcgatt tgcggaagac ctggaccaac tcttcttcca
    11641 actcaaggtg aggtgtaaca aggtcgagga acgggacaat ggttttcatt tcatcagcgc
    11701 cttttcttcg tcgagggagc gcatgaggcg cgcaggtact ccggcgacga tagtgttgtc
    11761 tggaacattt ctggtgacaa cgctgcctgc acccacaatt gcgttctctc caatgatgac
    11821 gcgggaaagg accgtcactc cggagccgat ggaagcacct tttttcacca gcgttttttc
    11881 cactacccag tctttttctg tctgcaactg gccatctgca gttgtggcgc gcgggtaaga
    11941 gtcgttaatg aaggtgacgt tatggccgac gaagacattg tcttcaatca caacaccttc
    12001 gcagacaaac gtgtggcttg atattttgca attgcgtcca actttagcat tcttctggac
    12061 ctcaacgaat gcgccgatct tcgtgttgtc tccaatctca caaccataga gattgatgaa
    12121 cctggacagt tttacgtcct tgcccagttt tacgtctggg gaaatgcaga caaaatcgtt
    12181 catattaaga cctgtgttcc ccggtttttt aaggattcgt cggcggcctg cagcattttc
    12241 acgattcgca gaccagagat gccgtcgttg atgggccgtt tatcttcgcg aatgcattcg
    12301 ttgaaatgcg cgatttccag cttgagcgcc tctgcgtttt caagctgcgg ggcccacatg
    12361 tcgccggagc ggtagctgac gagcaggtca tagatgccct cgcctgttct ggtgtgcttt
    12421 acgccacgat catagacctt gatcttttcg tcggattcaa ggtcgttcca gatcagcatc
    12481 tttttgtctc cgccgacaag tgtggtgcgc acctttacgg gagaaagcca atttacattc
    12541 acgtgggcga tcagcttgtt gggatagtag atcgtgacaa aggccacatc ttccacacca
    12601 ttcacatgct tctggccagt ggccacgact gcttcggcgc gttccggcaa aagataatcc
    12661 atgatggcga agtcgtgcgg tgcgaggtcc cagatgacat tgacgtcatg ctgaaagagg
    12721 cccagattga cccgcatagc gtcgtaataa tagatgtccc ccagttcgcg tgcctcgatc
    12781 agctgcttaa ttttttgcac ggccggcgtg aacagaaacg tgtggtccac catgattttc
    12841 aggcccttcc gttcggccag ctcgatcaat tcttctgcct gggcggaatt cgatgtaaag
    12901 ggcttttcaa caaagacgtg cttgccatga agcagggccg ccttcgccag ttcgtaatgt
    12961 gtccagaccg gagtgaccac cgcgataacg tcaatatgcg gtgaacggac catttccatc
    13021 gcctcggttg agacttctac atcgggatgg cttttcatga tacgggccag tgcttccggc
    13081 cggcggtcac acaccatggt gacctgcgag tgttcctgtg cgctaaagtt gcggacaata
    13141 ttcgggcccc aatatccgta gccaacgact ccaacgcgca gtacgtctga tctgagcgcg
    13201 ggttttgggc gtatttctgt ccagggcatt ggcatgtgct gtaaatgccc gagcatcttt
    13261 acatcaaaag acgtgtccgc gattacggga cgcacgtcaa ctgttactgg tatgtctccc
    13321 atcctgcctc cgagggggga tttgcataat ttccgcggta agcagtgcaa tcatgatgcc
    13381 aaagcatact ggctgataga catcagcatt tctggtcatc gcgttgtctc tatgcaagag
    13441 tttgctcaca ggagggcgca tggtccttag caggcagaag aaaatctttt ccttgaggta
    13501 aagtgagttc cccgaataag ggctggttgc ctgttattca ggcgtgcgga aactcatgac
    13561 gggccccact ggatggcaaa agaaggccag gaagccctct tttgcctcgg aaagcaggtt
    13621 tggtctggcg aatgcaagac agaagcacgt atgaaagtgt ctgtcatcct gggtactcgc
    13681 ccggaagcca ttaaacttgc gcctgtcatc ctggcattca gagccagcag agagtttgaa
    13741 tgtcatgttt gtgttacggc gcagcatcgc cagatgctag accaggtgct ggaagtcttt
    13801 cgtattgttc ctgatgcgga cctcgacttg atgaccgagg gccagacgtt gagcggcctt
    13861 acggcgcggg cgctgacagc gctggatgtg tatctggcgc gggaaaagcc agatctggtc
    13921 ctggtccagg gcgacactac aacggtgttc acggcctctt tggccgcttt ttaccatgga
    13981 atccctatcg ggcacgtgga agccggtttg cgcacctgga acctgcggtc tccctggcca
    14041 gaagaagcca accgtgttct aacaagccgt ctggctggtc tgcactttgc tccgactgaa
    14101 agcgcacggc agaatctttt gcgcgagggt attccggatg gcgacatctt tgttacagga
    14161 aacacggtca ttgatgcttt ataccttgcg gcagatcgcg cgcggggttc ttcgcctgag
    14221 attcccgggc tgccttttga attgatgatg gacggcacac cgctagtcct gatcacgggc
    14281 catcggcgtg aaagttttgg agaaaagctg gaagcgattt gcacggcaat ccttgacctt
    14341 gctgaaacct ttcctgaggc ccactttgtc tatcctgtcc acctgaaccc gcaggtccgc
    14401 aatacggtca tgcgtatgct atctgcgtca tcgtccggaa atattcacct gattgcaccg
    14461 ttgtcatacc ttccgtttgt agcgttgatg cagcgtgcga cgctcctgat tacggactca
    14521 ggaggcattc aggaggaagc gcctgcgttg ggaaagcctg ttttggttac acgcgacaca
    14581 accgaacgtc cggaagccct ggctggcgga acggtaaaac tggtcggtgc agatcgagcg
    14641 ggaattgtgc gtgaagcatc gagactgttg ggcgatgctg cataccttca tgaaatgtcg
    14701 cgcatttgcc tgccctatgg tgatggaaag gccaccgaga ggatcctggc cgcgattgcc
    14761 acgcgatttg acacagctcc ggtacaacag gaagaacttt tgcctgtggc ggtgggccgg
    14821 taatgacgct gcgcaaacgc attctcatga tcagtccgct gtttccgcct acggcggatt
    14881 ccgaggcatt ctgcgctggg aaaatggccc aggcgctgcg gaatactggc tgcgacgtag
    14941 aagtaatttt ctgcagcaat tatccgggtg cagtgcgcag ggacaattca cagttgtgga
    15001 aagacttttc ttctttttcg cacaatgttc cgctttcatt cggcagttgg accaggtctg
    15061 ttccgtcagc gctgcggttc ggaacagtct ttttctcccg gtgggtgagg gatgccgtcc
    15121 aattggccgg gcaactccat gcggcaaatc catttgattt tgtgcagtcc cggtcgttac
    15181 cgatgattgc gcatgttgcc ggacatcaca tatcgaaaga gttgggcatt ccgtggaacg
    15241 caaacatcaa cgatccctgg gactttcatc tctttccaga tgcgatgcgt gtgaagtggt
    15301 ccaaccgatg cgtccatgcc atacgtgctg cggcgcattt ccagcgtatt ggagttgttt
    15361 ccagctactg gatgcgaaag acgtttcgca ctgctgatct ggtgacttat tgttcggcgc
    15421 ggcttgaaaa gtatcacctt gcattggcag gcgttccgca tcgaagcgcc atcgtgccgc
    15481 atatcgggtg gagcgtgccc tgtgacggcg gccaagggct acatcttgtc catgcaggga
    15541 aattaggcgc gaatgaattt accggccgtt ccacccgaac acttctgcat ggaattgccg
    15601 catttgttca aaagcggcag ccggagcata cagaatttct tcttacattg gttggcccgg
    15661 aagacccaga gacagcaggt ttggtccgcg cgctcggact tgagaagttt gtgcgcagca
    15721 tcgggcctgt gagttatgag gagagcctgc gctggattgc gagcgcttct gtatgtgtgc
    15781 tggtagaggc ccgcatggac gagggaatct ttttcccttc taaatgcgcc gactatatcg
    15841 ctgccggaaa acccattctg gcgttcagcc cacaaacagg agtcattgcg gacatggcca
    15901 gtctcggcac cggcatcgtg caaatccgtg aaggcgacag cgatggcgtt gcggccgcat
    15961 tggagagatt ctgtcttgca caacaaaaag gaactctcga agagtttgct cctgatgcag
    16021 cgttgcggtc catgtgctcc ccggaagagg tcacaagagc atttctggaa acggcagggc
    16081 cacgtctctc gataggaaga gaaaaacagg ccgccagatt tcctctctgg gaaaaaactt
    16141 tcacatcccc acaaccagac ccaggcagcg cacaaaaatg acaagatttc ttcacctgct
    16201 gccaaggcct gtccagcaat ggatgcacaa caatcccatg gggcggcggc ttgcgcatgg
    16261 agcattctgg acccttttag gcacaatcgc ttcgcgagtg ctggccattc ctgtttctgt
    16321 cgtccttgca cgctggatgg ggccatccca ttacggcgaa ctgggtgtca ttaacaacag
    16381 cgttgacctt tttattgttt ttgcaggttt tggactaggc ctgaccgcga cgaaacacgt
    16441 tgccgaattg cgcacaacag accctgagcg cgctggacgg gtgttggcgt tatctacggt
    16501 aactgccgga tttaccgcga ccattacggc cgtcgtgctc tatatcgctg ctccctatct
    16561 ggcggcacat accctgaacg cgccgcagct taccggtcct cttcgcatag ggtctttgat
    16621 cctcctgctg gccgctatca acggagcgca acagggtgcg ctttacggat tcgaagcatt
    16681 ccgcacaacg gcgaaaatac aggcaattgt tggccttctg aaccttccat tcagcctgat
    16741 tggttattgg cttggcggat tgaacggcgt gctttggggc atggtggcga cccggggtgc
    16801 agactggttg cttcgccgcc tggctctgct ggatgagtcc cgcaagagtg gcgtgccttt
    16861 gggccttaaa ggttggacac aggaactcca tgttctctgg aaatacagcg tgcctgcgct
    16921 cttgtcgggc gtcatggttg cgccggtgaa ctgggtctgc gccgccatgc tggtaaacca
    16981 gcccaaaggc tatgcgcaga tgggcatcta taacgctgca aatcagtggt acggcgcatt
    17041 gcttttcctg ccgacggtgc ttgggagcgg gttactgcct ctgctttctg agcgcatggg
    17101 gaacaaagac agccactctt caggaaaagt tttgctcttt atgcttcgcc tgaatgcggt
    17161 catcgtcctg cccgcggcat tccttggttc agcacttagc ccctggatca tgcggctcta
    17221 cggcccggca tatgcgcatg gatggtccac gctggttgcg gtattgttca cggcggccgt
    17281 ttttgctgtc ctcattccgg taggagatgt tattgccgca tccggccgca tgtggaccgg
    17341 cacggtcatg aatgcaggct gggccgcagt tttcattgca ggcacggcgg tgctcgtcca
    17401 tcgaggagcc ttcgggctgg ggcttgcacg gcttattgcc tacatagcac acgcgatctg
    17461 gaccatcgct tttgcatgga tcctgatcac gcgggcaaag gcgagtcagc aggcaatttc
    17521 ttcggctgcg attactgccg gaagtgaggc cgcatgagga agtgcaattt cctgcacatc
    17581 tttggcgtgg acgatccccg gccgaacaat caacacgaat ggcacgtcac atgcaagtct
    17641 ctgcgttgag gacaaggcca gtttccgcac gactggaagc aaaacaatga aaaaaacact
    17701 gcttttcgca attgcactct gcctgatggt catgcgtccg gcgcaagcac agaacgcagg
    17761 ctggatggac cccgcctggt tataccgtaa tccggtcacg atcaataacc cgagcggtac
    17821 agcgctctca caattccagg tgaacatcca tctgggcagt gaaacgaact ttggggcggc
    17881 gaaaagcggc ggggccgatc tgcgcgtgac tgcgagcgat ggagtgacac agctcccgtt
    17941 ctggattgag agctggaacg cttcgtcctc gcaggcaagt atctgggtca aggtgccaag
    18001 cattcccact acgggcacaa cgctctttat ttattacggc aatcccaatg cggcgtctgc
    18061 ttcaaacggc aataacgtgt tcgagttctt cgacgatttt gaaacaccct acggcacgca
    18121 gactgggtat tttgcccaaa gcagccccac aactgtaatg gtgcaggacc agacgtggga
    18181 ggccaatcct ccgcatacgt taagcgtggt gcaggcaaac cagaatggtt atacatattc
    18241 cggctactac ggcacatacc aatgcgatgg catcggtctg gcctatagta atgatctgac
    18301 acactggaca aagtacaccg gcaatccgct catcagcggc gggcgctggc ccaccgtgca
    18361 actggtcaat ggtgtgtact acatgctgta tgaaaagaac ttttgcacca gcagcagcta
    18421 tatcgagctg gccaccagta cagatgggct ccatttcacg gacgtgaaac ctatcgtgaa
    18481 agcacagtct ggattaagaa accagaaccc gaacctgttc ttcaatccaa acgataatct
    18541 ctggtacctg tactactatc acggggatga ttcatcgaac ttcaatattt atgtgagaag
    18601 cgcattttcg atcaccgctc tggatacagc ttccacaact actgtgctga gttcaaaggc
    18661 aaaaatggcc gcccccagca tgttttatta caacgggaag tactatcttt ctactgaagg
    18721 aaccgatcct acccagcaat gggtcacgat agtgtattcg agctcttctc ctaaatccgg
    18781 ctttactctt gtgcccggga a
//
LOCUS       DASRBV010000274        14907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_322655, whole genome shotgun sequence.
ACCESSION   DASRBV010000274 DASRBV010000000
VERSION     DASRBV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322655"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VFA02_10090"
     CDS             <1..146
                     /locus_tag="VFA02_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZU10241.1"
                     /translation="QNSNERSQELRPWLHQYNWHRPHGSLGWSPPVSRSALDRNNLLR
                     LHI"
     gene            complement(2387..2463)
                     /locus_tag="VFA02_10095"
     tRNA            complement(2387..2463)
                     /locus_tag="VFA02_10095"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2427..2429),aa:Met,seq:cat)
     gene            complement(2570..5746)
                     /locus_tag="VFA02_10100"
     CDS             complement(2570..5746)
                     /locus_tag="VFA02_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU10242.1"
                     /translation="MKRERMRVQSFFPYLLIVVFSIFAANADAQVVGATISGTIRDAS
                     GAAVPQTTIIVHNLETGVERKVTSDGEGHYAVPSVPIGRYEVIAQKDGFSTQSRSGVN
                     LVIGQSAVVDFSLAVGQVHEEVAVTDTPQTVNPTTQPVSGLVNERQVKELPLNGRSFD
                     ELITLNPAVVNYTGQRSGSVGTTNSSPGNMFAVAGRRPQDNLFLLNGIEYTGASEINV
                     TPGGVSGQLLGVDAVREFNVVYDTYGAEYGKRTGAQVSIVTASGTNQLHGSAFEFLRN
                     SALDARNYFDQGNIPIFQRNQFGGALGGPLVKSKLLLFGNYEGFRQNLGLSNVTLVPD
                     NASRAQAVASVQPLLTLWPVQNGPELGGGIAEAFNHPLQRIREDFGTTRLDYNISQKD
                     SAFAVYTLDDSTAKTPTVNPLSTVDTDLREQVLSAQEQHIFSSNLLNTLRFGFSRGAF
                     FFTGETPVDVQGWVAGKPIGAIVIGGGTALNGASQISLAGTNAGSNLHAVRNLFTWDD
                     HVFISHGKNQLEAGIWLQRIQSNSNLAQYQYGQASFSSLSAFLNGTVSTFTVVPSPTE
                     LGWRSLEGAGFVQDTLRLWPNLELRLGFRFESTNGWNEAQGRASNYIFDQNGVLETNP
                     RVGDSAFTENRAKFLPEPRIGLAWSPRQNGTTIVHAGFGIYRALLDSLDYRLDQNAPF
                     NTTQSYKNIQVANLHFVPGQSAPSGGRVSPSGIEPDAHTPTLLSWSLNIEQQIAPATS
                     LTIGYIGSHGYHQILSADLNEPVPVICPASPCPSTLAAGTVYYPKGSPLANPNLANST
                     TWISRGVNSYNGLTVDVNRRFQNDLQLRGVYTFSKNLDEGTAWNSSVGANAPGFVMYP
                     ARPDWDYGPANTDVRHLAVINGTYELPFGQSKHWLHGGGFWQQKVIGGWTTSAIVNLQ
                     SGLPFTPQLGFNPSNNGDSRNPVRPSVNPDFRGKVIEGKPSQYFNPNAFIVPVSGTYG
                     NLGRNTLTGPGLSSLDISLRKNTAITEKLDLQFRSEFFNILNHTNFGTPNTVVYSSAS
                     SAPSPTAGVITTTATTSRQIQFGVKLLF"
     gene            complement(6027..7421)
                     /gene="hemG"
                     /locus_tag="VFA02_10105"
     CDS             complement(6027..7421)
                     /gene="hemG"
                     /locus_tag="VFA02_10105"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZU10243.1"
                     /translation="MRTAIIGGGIAGLSAAYELARTGKEFVLYEATQRLGGIIETVRR
                     DGFVVECGPDSWVAEKPWARELAIEVGLAGEIIPSNDHLRQTYIVQGKSLVPMPDGMR
                     MMVPANIEALLHSPLFSEEAKRAYLREPGRAAELKASAPAEDTDESVSSFVRRHFGDE
                     VTDTIAAPLLAGVFGGSIDRLSARAVMPAFIKMEREHGSLMTALQKHIHESEKSAIFA
                     TLKPGLQTLIDRMAEALPATSVRLGMPVSGLSWNNGRWSVSCTAGSEQFDAVIVAAPA
                     DVTRSLLRPLDFAFDELLAMDASSAIVIALAFPPERAKSMRIPRGFGFLVPPAKPGQM
                     RDPQLLACTFVDQKFSHRAPDGAVLLRAFFGGEDAPQLLKRTDEEVAALACRQLSLLL
                     GDLPEASFTVIRRWPRSLPQYAVGHMARMKQLADLAQSFAGLYLIGNAYYGVGLPDLI
                     REGRKAARQIADHN"
     gene            complement(7418..8341)
                     /gene="hemH"
                     /locus_tag="VFA02_10110"
     CDS             complement(7418..8341)
                     /gene="hemH"
                     /locus_tag="VFA02_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZU10244.1"
                     /translation="MADRMAVLLLAHGTPDSLDEVPEYLRNVTGGRPIPDAVIEEVRH
                     RTSMIGKSPLTKLTQEQGRLLAKELGVPVYVGMRNWRPYIADVVKQMLEDGTTSAVVI
                     CLAPQNSRTSVGLYKRAVMQAVEDRMRILFIEAWADHPLLAEAFADRLRPVWERVSNE
                     TGAPVPVVFTAHSVPQRTVEAHDGQPADPYAEEAKRTAASVAERVPELRHWRFAFQSQ
                     GMSGGPWLGPTVEETLTALHQEGHKAVVIQPIGFLCDHIEVLYDIDIAFREFGKQLGM
                     RIERPESLNNSPLLIGALADIARQGLAQLAS"
     gene            complement(8334..9419)
                     /gene="hemE"
                     /locus_tag="VFA02_10115"
     CDS             complement(8334..9419)
                     /gene="hemE"
                     /locus_tag="VFA02_10115"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZU10245.1"
                     /translation="MQQSTSHLQIEADSLTSGTRFVRACLRRPVDRTPVWFLRQAGRY
                     MPEYQAVRKHHSLLEICKTPALAAEVTITAAEKLDVDAAIIFADLLLPLTPMGLDFEF
                     QAGEGPVVHTPLRTAEDVARLRTDRASELGYVAEAIEKVAAHFKDRIGVIGFCGAPFT
                     LASYMIEGGSSRNYIETKKMMYRQPAVWRSLMDKLTKVLREYTKQQVEAGADVIQIFD
                     SWAGVLSIEDYRDFILPQTRTLVREVQSLGVPVIYFGVETASLLPSMRETGADVLGLD
                     WRAPLGHTWRALDYSCAVQGNLDPITLFAEPELIRKRVRQILHEAAGRPGHIFNLGHG
                     IVPGTPVENVQHVVRFVRELSSEVKHG"
     gene            complement(9479..10192)
                     /locus_tag="VFA02_10120"
     CDS             complement(9479..10192)
                     /locus_tag="VFA02_10120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU10246.1"
                     /translation="MPELNGQVAVVTGAGRGIGEAISKRLAVMGATVVLTGRDLAQID
                     RVKLDIRAKGGIAETAHLDLLDDSSVTRLAAHVQSRYHRCDILVNNAGIGLTGKPLHE
                     TTPDEWDTLMGTNLRGPYLMVRAFAPIMITTRSGHIINISSLAGHNPLPDGAAYSASK
                     WGLNGLTYSLAEELRPYNIRVSVIAPGSVNTHFNVSSGKDPNKKIQPEDVANVVAMLV
                     TQAPQSFVSEVLLRPTQKP"
     gene            10338..11069
                     /locus_tag="VFA02_10125"
     CDS             10338..11069
                     /locus_tag="VFA02_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZU10247.1"
                     /translation="MKTRLDKLLVARGFAPTRERAQALVLAGRVLVNEQKEDKPGHNV
                     QEDAIVRILGEDLRYVGRGGLKLEAAIKHWKIDLAGRFCLDVGASTGGFTDCMLQHGA
                     ARVLAVDTGYGQIAQKLRDDQRVQLMERTNARHLQPGSLPESISFLAMDVSFISATLV
                     LPAVRAAAPGLTEAVVLIKPQFEAGREHVGKGGIVRDSEAHAIAIEKVRGCVDSLGGQ
                     GTEVMESPVLGAEGNREFLLHARFL"
     gene            11125..11982
                     /locus_tag="VFA02_10130"
     CDS             11125..11982
                     /locus_tag="VFA02_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZU10248.1"
                     /translation="MRRIAIISKPQKEELTTLLPQLVLWLRAHGFTPVLDPVSGNYAQ
                     EKNVVPRGELPEQNPELVIVLGGDGTLLAAARVFAKTSVPILSVNLGSLGFLTEVRLG
                     DLYSTLQGWCDDCCAIDTRVLLHSELWRDGKMVADHEALNDVVVAKGAIARMVNFTIR
                     LDGQLVAAFRADGVIVSTPTGSTAYTLAANGPIVVPNVDALVLTPVCPHLLTLRPLVV
                     RGDTSLSVCVEGVPDQTYLTVDGQEAVPLRLGDELRCRRSDHYVRLVRMGSTGFFDVL
                     RSKLKWGER"
     gene            complement(12204..13166)
                     /locus_tag="VFA02_10135"
     CDS             complement(12204..13166)
                     /locus_tag="VFA02_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZU10249.1"
                     /translation="MNRKLDLKAFAALILAASLGLPALAHAQSDQQTAPSSASSAQVQ
                     TGTSANGMNPNNNSTYATGQPLPTKSNEGFWGHMNPFARKKWVNRQLNPVKDRLNELD
                     QLTAKNANDIKDVDSRAQAGIRQAQSTADQASQTAQQANSTATQANTLAQQASDQSNK
                     LNTTIAGLDQYQKVNDTIIRFRPGQSVLNARAKDALDQIATQAQGQKGYIIEVEGHSP
                     VRGQAGIQSSQRMADAVVRYLVTEKQIPVYRIHQVAMGNAKMKTDSGETISGSAVRVS
                     TMQNSLAALNSTSSNAGSPIGATQQSSAQPSPQGAASQPAVPQQ"
     gene            13525..14595
                     /gene="dinB"
                     /locus_tag="VFA02_10140"
     CDS             13525..14595
                     /gene="dinB"
                     /locus_tag="VFA02_10140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZU10250.1"
                     /translation="MTVRKIIHVDMDAFYASVEQRDDPNLRGKPVIVAWRGNRSVVCA
                     ASYEARKFGVHSAMPAVRAERLCPDAVFVSPDFTRYRAVSRQVREILQRHTDLIEPLS
                     LDEAYLDVTENKTGLPTATRVAIAIRKQIREELNLTASAGVAPNKFLAKIASDWKKPD
                     GLFVIQPEEVDSFLLPLSVGRIPGVGKVTQKHLEQIGVKTIAQLRSLELSLLEEHFGR
                     YGHRLYELARGIDLSPVISDRPTKSISAEDTFERDMTLDETGEIIRRLAERVWSASRK
                     DARPARTVVLKLKTAEFRILTRSHTLTGPLASCEELTSIALSLRERVGLGPEQRFRLV
                     GVGLHNFRDPEDPQEQPALFEA"
     gene            complement(14658..>14907)
                     /locus_tag="VFA02_10145"
     CDS             complement(14658..>14907)
                     /locus_tag="VFA02_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZU10251.1"
                     /translation="LLRQKGAIVSYNDPFFPYVGRGRHYNLDMHCTPLDDLSQFDCVL
                     IATDHSDYDYERIVRESQLVVDTRNATKGISSPKIVRC"
BASE COUNT         3350 a         3850 c         4307 g         3400 t
ORIGIN      
        1 atcagaactc aaacgaacgc agccaggagc ttcgcccctg gctacaccag tacaactggc
       61 accggcccca tggtagtctc ggctggtcgc cgcccgtcag ccgatcagct ctcgatcgga
      121 acaacctgtt gagactccac atctagaaga aacagtgttc agcctcatga tttttcaaag
      181 taagaaaagc agagcgaagc gtcagtgagt cgtgttgtcg gttggctcaa ttcacgccat
      241 atgtgaagaa gatgaacgtc gccatgaccg ttctctgtga tggtctgctc tgatcatcca
      301 ctttgaggga tgagcatgaa ggttaagcac cagccttttg cgccagagca ggagaaaaat
      361 accgggcaca gagcaaaaag ttcacaattg ctcgcaattt gtagtcaaac gaagcaaatc
      421 acttcggaag tgaggacaaa gggccctatg cacgggatct gaacgaccag gagatttgtg
      481 cctctttttg gcgtctagtg aggcctgaga tagcggatgc ccatgtgcag acatcggcgt
      541 caattgctcg tgccccacaa gccaactatg ccggttcgga gacgtcttga cctcgttctt
      601 tgatcacgtt acgttgatca cgctggccgt tcggaagaac tttgccgggg gtgaaaaaaa
      661 gcttttccaa aatatctcct ggaactgtcc gagcagtatt acctactgtg cgggcaaaca
      721 caaaatctga gtctccagct aaagttcggg atacgctgga gcgcgagaag gagccaaagc
      781 caatctcacc ggtgcttcgg tcctggcttg acggggtcat catcccgata atcgtgaggg
      841 aacttcttgg tgattagata cgttatttat gcacgatatt cctcggatca acagtcgcgc
      901 tgttccattg cagaccagca gcgcaagtgc agggaatatg gtgttcgcga gggttggcat
      961 gaggtgaggg cctacgagga tgaagccgtc tccggtgtag gctcagatcg cgtcggcttg
     1021 gaaaaactca tggcagatgc ttgtgaccct ctgcgggatt tcgatgtgat tctcgtcgat
     1081 gacacctccc ggcttagccg ctcactgccg gaagtagtaa cgctccacca gcgtctctca
     1141 catcacggcg ttcgggtcat cgccgtttcc cagggaattg acaccgagca tgagcagagc
     1201 gaggttctca ttgctatgca cggcatcacc gacagcttgt acgtgaagga acttgcgaag
     1261 aagacacacc gagggctaga gggcaagttt ctgaatggct tgtcagcggg cgggcgctgc
     1321 ttaggttatg cttcggtccc ggtcgagagc ggcggagtac gctgggtaat caacgaagaa
     1381 gaaaaaaaga ttgttattga aatatttgaa ctgagcgcag ggggttcttc gctgaaatcg
     1441 atcgcgggga ttcttaatga gagtaagatc cctccgccgc agaagcgagc cgatcgtccc
     1501 catccgacat ggtgcccttc ggcaatccgg gagatcctac gccgcgaact ttacataggc
     1561 aaacgcattt ggaatcgacg aaagtatgtc aagacgccag gaacgaataa acgggtgtcg
     1621 cgacttagac cgcgaagtga atggaaggtg caagaggttc cagaactccg gatcgtgagc
     1681 gatgaactgt ggatccgcgt ccagaagcgt caaaattttc tgaaggagag atacgcagag
     1741 agcggacgta agccggtcaa tcgggcatcc tcatctgcgt atcttttctc tggattcctc
     1801 aggtgcggct cttgcggtgc gaacctgatc atagtctcgg gtagtggtaa aggggcgagg
     1861 tatgggtgcc cacagcattg gaacaggagg gcatgcccca acagaattac agtccgcagc
     1921 aacgacgttg aagttcagct tcttgccggg ctacagagtg aggtgctcag cgacaaagtg
     1981 gtcgatgctg tggtggaagg cgcattgcgg gccgttcgca agcgagatac cagcagtcat
     2041 cacgtagtca gaatccgcga gttggaggcc gagatcaagc ggttggtttt ggcgattgcg
     2101 aagttggaaa actctgacgc gatgctcagt gctgtcacag agcgagaaac tgaacttcgg
     2161 gaactgaagg cagcaagggc cttcaagact gaattaactg aagaggctat ccgttcctcc
     2221 gttcacaaag cgctatccga tattcctgaa ttacttaaga aagcgcctga aaaggcaaaa
     2281 gccaaattgt cggagcatct gaacgaaatc gagatgattc cgcagtcaaa tggctcgtat
     2341 cttgctcttg gagaatggga cctgctcgga gtccgggggc cagtgttggt tgcgggggct
     2401 ggatttgaac cagcgacctt tgggttatga gcccaacgag ctaccaggct gctccacccc
     2461 gcacttcaat attaacaacc gttaacgaag cgggtcaaac gagcagcctg tacatctcgg
     2521 tatgcagtgg agtgtggcgc aaaatggcag cacccaccac ggttttcact cagaaaagca
     2581 gcttcactcc gaactgtatc tgccgtgaag tggttgccgt tgtggtaatg actcctgcgg
     2641 ttggcgacgg agccgaggag gcagacgaat acaccactgt attgggcgtt ccaaaattgg
     2701 tgtggttcag gatattgaaa aattcgctgc ggaattgtag atccagcttc tcggtgattg
     2761 ccgtgttctt gcgcagagaa atatccaggc ttgaaagccc cgggcctgtc agcgtattgc
     2821 gccccagatt tccatacgtt ccagagacag gaacgataaa cgcattcgga ttgaaatact
     2881 gggatggttt gccctcaatc actttgccgc gaaaatcagg gtttacggaa ggtcgtaccg
     2941 ggtttcggct atctccgtta ttggacggat taaagccaag ctgcggcgta aagggaagtc
     3001 cggattgcag gttcacaatg gcgctcgtgg tccatccacc gatgactttc tgttgccaga
     3061 agccgccgcc atgcagccag tgcttgcttt gcccgaaagg aagctcgtat gttccgttga
     3121 tcacggcaag gtgacgcaca tcagtatttg ccgggccgta atcccagtca ggccgcgcag
     3181 gatacatcac aaacccagga gcatttgcac caacactgga gttccatgct gtgccttcat
     3241 ccagattctt tgagaaggtg taaacgcctc gtagctgaag gtcattctga aagcggcggt
     3301 ttacgtctac tgtcaggccg ttataggaat tgacgccacg cgatatccac gtagtgctgt
     3361 ttgccagatt tggattggca agtggcgagc ctttcgggta gtaaaccgtt ccggcagcaa
     3421 gcgtagaagg gcatggcgaa gcagggcaga tgactggaac cggctcattc aaatcagcag
     3481 aaagaatctg atgataacca tgtgatccga tatagccaat ggtgagtgat gttgcaggag
     3541 cgatttgctg ctcgatattc agtgaccacg aaagcagcgt aggagtatgc gcgtccggct
     3601 caatgccgct gggtgagacc cgtccgccgg agggcgcaga ctgtccgggc acaaaatgaa
     3661 ggtttgcgac ctggatgttc ttgtagctct gcgtggtatt gaatggcgcg ttctggtcaa
     3721 ggcgatagtc aaggctgtcg agcagcgcac ggtaaatacc gaagcctgca tgaacgatcg
     3781 ttgttccatt ctgccttggc gaccaggcca gaccaatacg cggctcggga agaaactttg
     3841 cgcggttttc agtgaatgct gagtcaccga cgcgcgggtt tgtttcgagc acaccgtttt
     3901 gatcaaagat gtaattcgat gcgcgtcctt gcgcctcgtt ccatccattc gttgattcaa
     3961 aacggaagcc aaggcgcaat tccaggtttg gccataagcg cagtgtgtcc tgcacgaagc
     4021 ctgccccttc gagtgagcgc cagcccagct cagtgggcga aggaacaacg gtgaaggtgg
     4081 aaaccgtccc gttcaggaat gcactcaggc tgctgaagga cgcctgtcca tactgatatt
     4141 gcgccaggtt gctgtttgat tggatgcgtt gcagccaaat gcctgcttcc agctggttct
     4201 ttccatgcga gatgaagacg tggtcgtccc acgtaaagag attgcgcact gcatgcaaat
     4261 tgctgcctgc attcgtgcct gcaaggctga tttgcgatgc gccattcagc gcggtcccac
     4321 cgccaatcac gatggccccg ataggcttgc cagcgaccca tccctgaacg tcaacgggag
     4381 tttctcctgt aaagaagaat gcgcctcgtg aaaaaccaaa acgcagagta ttcagaagat
     4441 tggaagagaa aatatgctgt tcctgtgcgc tcagtacctg ctcacgcagg tctgtatcta
     4501 cagtgctcag cgggtttacc gttggggttt ttgcggtgct gtcatcgagg gtatagacag
     4561 cgaaggccga atccttctgc gagatgttgt agtcaagccg tgttgtgccg aagtcttcac
     4621 gaatgcgttg aagtggatga ttgaatgctt ctgcaattcc gccgccaagc tccgggccgt
     4681 tttgtactgg ccagagtgta agcaacggtt gtacactggc gacggcctgt gcgcgcgaag
     4741 cattatcagg cacaagcgtc acatttgaga ggcccaggtt ttgacgaaag ccttcatagt
     4801 ttccaaagag cagtaacttg ctttttacca gcggaccgcc cagcgctcct ccgaattgat
     4861 tgcgctgaaa gatgggaatg tttccctggt cgaaataatt gcgggcatcg agggcactgt
     4921 tgcgcaaaaa ctcaaaagcg ctgccgtgca attgatttgt gcccgatgcc gttacaatgc
     4981 tcacctgtgc cccggtgcgc ttgccatact ctgcgccgta ggtgtcatac acaacattga
     5041 attcgcgtac tgcatctacg ccgagcaact ggccgcttac acctcccggc gtgacgttaa
     5101 tttcagatgc gccagtatat tcaattccat tcagcagaaa gagattgtcc tgagggcggc
     5161 gtccggcaac ggcgaacata tttccaggag aagaattggt tgtgcctacg gacccggagc
     5221 gctgccctgt gtagttcacc acagcgggat tcaacgtaat cagctcgtcg aagctgcggc
     5281 cgttcagtgg gagttccttt acctggcgct catttacaag gccggagaca ggctgggttg
     5341 tggggttgac cgtttgcggc gtatcggtga cagcaacttc ttcgtgcacc tggcctacgg
     5401 caagtgagaa gtccacgact gcactttgcc cgatgaccag atttacaccg ctgcgcgact
     5461 gtgtgctgaa gccatctttt tgggcaatga cttcatagcg tcctataggg accgagggta
     5521 cagcatagtg gccttcgccg tcgctggtga ctttgcgttc cactcctgtc tccagattgt
     5581 ggacgatgat ggttgtttgc gggactgcgg ccccggaggc atcccggatc gttcctgaga
     5641 tggtcgcgcc cactacctgg gcatctgcat ttgccgcaaa aatcgaaaac accactatca
     5701 acaaataggg gaagaaactt tgaacgcgca tgcgttcgcg cttcataaaa aactcctcag
     5761 atcaaaattc gtgaaaaaca aagaaaaggc aggaagggtc aacagcccat gaggactggg
     5821 ttcggcaggt atgtgaataa aagctctaac atgctttact cgaaaccaga gaagaaccag
     5881 ggaaattttt atttgcggcg gaacggcagg agagatttag ttcgcgcgca tacacgacgg
     5941 agcacagttg ccgacgcaac aggtacaggg ttcgctgctc cgaactggat acataaaaaa
     6001 agtgtaatgg cataaaggat tgcaggtcaa ttgtgatctg caatttgccg tgctgccttg
     6061 cggccttccc ttatcaggtc aggaaggcct acaccgtagt aggcattacc gatgagatac
     6121 aagccggcga acgattgtgc caggtcggca agctgcttca tacgggccat gtgtccgacg
     6181 gcatactggg gcagggaacg cggccagcgg cgtatgaccg taaatgatgc ttccggcaag
     6241 tcaccaagta agagagagag ctgccggcat gcaagtgcag caacctcttc atctgttcgt
     6301 tttagcaatt gcggagcatc ctcaccacca aaaaaggcgc gcagaagcac agcgccatcc
     6361 ggcgcgcgat gcgaaaattt ttggtctacg aaagtacagg cgagcaactg gggatcgcgc
     6421 atctggcctg gttttgcggg aggcacaaga aatccgaatc cacgtggaat gcgcatgctc
     6481 tttgcccgct ctggagggaa tgcaagggca atgacgattg cggaggaggc gtccatcgca
     6541 agcaattcat cgaaggcaaa atcgagaggg cgaagcagag agcgcgttac atctgccgga
     6601 gccgcgacaa tgacagcatc gaactgttcg cttccagcag tgcaagagac actccatcgg
     6661 ccgttgttcc atgagagccc agatacaggc atgcccagac gtacagatgt tgcaggcaag
     6721 gcctcggcca tccgatcaat caatgtctgc agtccgggtt tgagcgtagc gaagatcgca
     6781 gatttttcag attcatgtat atgtttctgc agggctgtca tcagcgagcc atgctcacgc
     6841 tccatcttga taaaagctgg cattacagcg cgtgcgctga gccggtcaat gcttccacca
     6901 aaaacacctg caagcaatgg agccgcaatg gtgtctgtga cttcatcccc gaaatgcctg
     6961 cgtacaaagc tggaaacaga ttcatctgta tcctcggcag gcgcgctggc cttcaattct
     7021 gcagcgcgtc cgggttcgcg caggtaggcc cgtttcgctt cttcgctgaa aagcggcgaa
     7081 tggagcagcg cctctatgtt tgcagggacc atcatgcgca tgccgtcggg catgggaaca
     7141 aggctttttc cctgcacaat gtaggtctgc cgcaaatggt cgttggatgg aatgatttcg
     7201 ccggcaagcc caacttcgat ggcaagctcg cgcgcccagg gcttttctgc gacccaggaa
     7261 tcagggccgc actcgacgac gaatccatcg cgacgcaccg tttcgatgat tccgccaaga
     7321 cgttgcgtgg cctcgtacag cacaaactct ttgcctgtgc gggcaagctc atacgctgca
     7381 gaaaggccgg caatacctcc gccgataatt gcagtcctca tgatgcaagc tgcgccagac
     7441 cctgccgcgc gatatcagcc agagctccaa tcagcagcgg cgagttgttc aatgactcag
     7501 ggcgctcgat gcgcatacca agctgttttc cgaactcacg gaaggcaata tctatgtcgt
     7561 acagaacctc tatgtggtcg cagagaaatc cgatgggctg aatcacaact gccttgtgtc
     7621 cttcttgatg cagtgcagtc aatgtttctt ctactgtagg gccaagccag ggaccgcctg
     7681 acatgccctg gctctgaaat gcgaagcgcc aatggcgcaa ttcgggaacc ctctccgcaa
     7741 cagaggcggc tgtgcgtttt gcttcttcag catatggatc ggcaggttgt ccatcatgcg
     7801 cttccactgt tctctgcggt acgctatgcg cagtaaatac gacaggaaca ggagcgccag
     7861 tctcgttcga aacgcgttcc cacaccggac gcaggcgatc ggcaaaggcc tcggcgagca
     7921 gcggatggtc tgcccatgct tcgataaaga ggattctcat gcggtcctct acagcctgca
     7981 ttacggcccg cttgtaaaga ccgacgctgg tgcgtgagtt ctgcggagcc agacagatga
     8041 ccacagcaga tgtggttcca tcttccagca tctgctttac aacatctgca atgtaaggac
     8101 gccagttacg catgcccaca tatacgggaa ctccgagttc ctttgccagc aagcggccct
     8161 gctcctgtgt aagcttcgtc agcgggcttt tgccaatcat ggacgtacgg tgacgcactt
     8221 cctcaatcac tgcgtcagga atggggcggc caccagtcac gttgcgcaga tattcgggta
     8281 cttcgtcgag cgaatccgga gtgccatgtg ccagcagaag aacagccatg cggtcagcca
     8341 tgcttcacct cactggacag ttcacgcaca aagcgcacaa cgtgctgcac attttctaca
     8401 ggcgttcccg gaacgatgcc atgtccaaga ttgaagatgt gcccaggcct gcctgcggct
     8461 tcatgcagaa tctgccgcac gcgtttgcgg atcaactctg gctcggcaaa gagcgtgatg
     8521 gggtccagat ttccttgcac ggcacaggag taatccaatg cgcgccatgt gtgtccgagc
     8581 ggagcgcgcc aatcaagccc cagcacgtca gctccggttt cgcgcattga ggggagaagg
     8641 cttgccgttt ccactccaaa ataaattact gggacgccaa gcgattgcac ctcgcgcaca
     8701 agggtcctgg tttgaggaag gatgaaatcg cgatagtctt cgatgctcag taccccggcc
     8761 cagctatcga atatctggat gacatcggcc ccggcctcaa cctgctgttt tgtgtattcg
     8821 cggagtacct tcgttagttt gtccatcaga ctgcgccaca cagcgggctg gcgatacatc
     8881 atctttttgg tttcgatata gttgcgcgag ctgccaccct ctatcatgta gctggcaagg
     8941 gtgaaaggcg ctccgcaaaa cccaataact ccgatgcggt ctttaaaatg cgccgcaact
     9001 ttttcaatgg cctcggccac ataccccagt tcagaagcac ggtctgtgcg caagcgggcc
     9061 acatcttcag ccgtgcgaag aggcgtatgg actacaggtc cttcacctgc ctgaaattca
     9121 aagtccaacc ccataggcgt gagcggaagg agcaaatccg caaagatgat ggctgcgtcc
     9181 acatcaagtt tttctgcggc cgtgattgtc acctccgcag caagcgccgg ggttttgcag
     9241 atttccagca gggaatggtg cttgcggacg gcctgatatt ccggcatata gcgccccgct
     9301 tgccgcaaaa accacactgg agtgcggtct acgggacggc gcaggcaggc acgcacaaaa
     9361 cgcgtacctg aagtaaggga atcagcttca atctgtaagt gcgaagtact ttgttgcatt
     9421 gcagagacca ctcttgagag tagcattgat tggagattta acctcgcatt cagcctgtct
     9481 aaggcttttg cgtaggccgc agcagtactt cactgacaaa agactgcggg gcctgtgtga
     9541 ccagcatggc gacgacattt gctacgtctt ccggctggat ttttttgttg gggtctttgc
     9601 cgctgctcac gttgaaatgc gtgttgacag acccaggtgc gataaccgaa acgcggatgt
     9661 tatacgggcg caattcctct gcaagcgagt atgtcagacc attcaggccc cattttgagg
     9721 cagagtaggc tgctccgtca ggcaatgggt tgtgtccggc gagcgatgaa atattgatga
     9781 tgtggccaga acgggtagtg atcattatcg gcgcgaaggc gcggaccatc agataagggc
     9841 cgcgcaggtt cgttcccatc agagtgtccc attcatcagg tgtggtttca tgcaggggtt
     9901 ttcctgtcaa gccaatcccg gcgttattca ccaggatgtc gcatcggtgg taccggcttt
     9961 gcacatgcgc tgcaaggcgc gtaacagagg aatcatccag caggtcgaga tgggctgttt
    10021 ctgcgatgcc gcccttggca cgtatatcca gctttacgcg atcgatctgc gcaaggtccc
    10081 gtcctgtcag gacaacggtg gcccccatga cagcgagacg cttgctgatg gcttctccga
    10141 tgccgcgccc ggccccggta acgactgcga cttgtccgtt gagttctggc atatcttttt
    10201 aaagatgcgc gtcccgtggt ccaggtgaca ggggacgcgc agccgattag cggtattggt
    10261 gttctgctgg acgggtttct cacctataag tgacccattc gtccatcaaa ccgatattct
    10321 acgtatgcat cgtttttatg aaaacgcgtc tggacaaatt gctggtggcg cgtggctttg
    10381 cgccgacgag ggagcgcgcg caggcgctgg tcctggctgg acgggtgctg gtgaatgagc
    10441 aaaaggaaga caagccggga cacaatgttc aggaagacgc aatcgtccgc attcttggtg
    10501 aagacctgcg ttatgtgggc cgcggcggcc tgaagctgga agccgccatc aaacactgga
    10561 agattgatct tgcagggcgg ttttgtctgg atgtaggcgc gtctacgggt ggtttcactg
    10621 actgtatgtt gcagcatgga gcagcgcgcg tgctggcagt ggatacagga tatgggcaga
    10681 ttgcacagaa gctgcgcgac gaccagcgag tgcagttgat ggaacggacg aatgcgcgcc
    10741 atctgcaacc gggaagcctg ccggaaagca tcagttttct ggcaatggat gtgtcgttca
    10801 tttcggcaac gctggtgttg cccgcggtcc gggcggctgc gccgggcctg acagaagctg
    10861 tagtgctgat aaagccccag tttgaagctg ggcgcgagca tgtaggcaag ggaggcattg
    10921 tgcgcgattc ggaagcccac gctatcgcca ttgaaaaggt gcgaggatgt gttgattcat
    10981 tgggtgggca agggacggag gtgatggaat cccctgttct gggcgcggaa gggaacagag
    11041 aatttctgct acatgcacgt ttcctctgaa tccggcgcga atcatctctg acctttcgca
    11101 ccgctgcgag tacactggac tttcatgcga cgcatcgcca tcatctcaaa gccgcaaaaa
    11161 gaggagttga cgacattgct gccccagctt gtgctgtggc tgcgtgcgca cggattcacc
    11221 cctgtgctcg accctgtaag cggcaattac gcgcaggaaa agaatgtggt gccccgcggt
    11281 gaattacctg agcaaaatcc ggaattggtg atcgtgcttg ggggggatgg tacgctgctg
    11341 gcggccgcgc gcgtctttgc taaaaccagc gtcccaattc taagcgtaaa cctcgggtcg
    11401 ctgggatttc tgacagaagt gcgcctgggg gacctctatt ctacgctgca gggatggtgt
    11461 gatgattgct gcgcgattga tacgcgcgta cttctgcaca gcgaactctg gcgcgatgga
    11521 aagatggtcg cagaccacga agccttgaat gatgtcgttg tggccaaagg cgccattgcc
    11581 cgcatggtca acttcaccat ccggctggac ggtcagcttg tggcggcctt tcgcgccgac
    11641 ggggtgattg tttccacacc taccggatca acagcctata cgcttgccgc aaatggcccc
    11701 attgttgtgc ctaacgtaga tgcgctggtg ctgacgccgg tgtgtcctca cctgctgacg
    11761 ctgcgtccgc tcgtagtacg cggtgatacg agcctgagcg tgtgcgtcga gggtgttccc
    11821 gaccagactt atttgaccgt ggacggccag gaggcggtcc cgttacgtct gggagatgaa
    11881 ctacgctgcc gccgttctga ccactacgtg cgtctggtgc gtatgggatc taccggattc
    11941 tttgatgtgc tgcgctcgaa gctgaagtgg ggagagagat aaagcacagc aggcgtcatt
    12001 ccgtagaggg cataatttac gacattttca cagtgcagtg tcaccggctc cctgctggcc
    12061 gtgtggcaac cgtagtttcg gggcatggtt cagactatct ttgaaaatgc tataaggcaa
    12121 aaaaaacggc ccctcggggg aggggccaat ctgccttggt tctctcgttg tgtgagagct
    12181 tggttgcgga ccttacgttc ttgttattgt tgtggaaccg ctggctgcga cgcagcaccc
    12241 tgaggggagg gctgcgcgga agactgctgc gtcgcaccaa tcggcgaacc cgcattcgag
    12301 gatgtcgagt tcaaggccgc taaactgttc tgcattgtgg aaacccgcac ggcgctgccg
    12361 gaaatggtct caccgctgtc ggttttcatt tttgcgttgc ccatcgcaac ctgatggatg
    12421 cgatagaccg gaatctgctt ttcggtcacg agatagcgga caacagcatc agccatgcgc
    12481 tgagagcttt gaattccagc ctgcccgcgt accggcgagt ggccttctac ctcaatgatg
    12541 taacctttct gaccctgtgc ctgagttgcg atctggtcaa gggcatcttt ggccctggca
    12601 ttcaaaacgg actgccctgg gcggaaacga ataatcgtat cgttgacttt ctgatactga
    12661 tccagtccag caatcgtggt attcagcttg ttggactggt cgctggcctg ctgtgccagt
    12721 gtattggcct gcgttgcagt ggaatttgcc tgctgcgcag tttgactggc ctggtcggca
    12781 gtgctctgcg cctgacgaat tcctgcctgc gcccgcgagt caacgtcttt aatatcgttg
    12841 gcgttctttg ctgtcagttg atccagttcg ttcagacggt cttttacagg attgagttgc
    12901 ctgttgaccc actttttccg ggcgaatgga ttcatatgcc cccagaagcc ttcgttcgac
    12961 ttggtgggca gcggctgtcc ggtcgcatag gtgctgttat tgttcgggtt catgccattg
    13021 gccgatgtcc cggtctgcac ctgagcgctg gaggccgagg agggggctgt ctgctgatct
    13081 gactgggcgt gcgccaaggc cggaaggccc agacttgcag caagaatcaa cgcagcaaat
    13141 gctttgagat cgagtttcct gttcattttg ttctctctca tattcatcac cgtttgtatc
    13201 cctttacgtt atgactcgcg tggaacacac tccggatgct gcgcagcgtc ccgttcacag
    13261 agagaattag cagttcaggt gccaaagtcg gaagtgtcgg aaaagaaatt gttaatggtt
    13321 ttaatttcat gtacttgcac aagaaatcca ttcatctagg accattcggg tcgtgtgctt
    13381 tctgtggcct ctgccaactt ttacatggtc acttttcctc tgtgccggaa atttttgctc
    13441 ggcattcacg ttgggctgag ggtcctggaa aaaggacaga aatccaagtg ccattcaggc
    13501 gaaagtaatg cgaaaattcg agcagtgact gtccgtaaga tcatccacgt tgatatggac
    13561 gcgttttacg cctcggtgga gcagagggac gatcccaatc tgcgcggaaa gcccgtgatc
    13621 gttgcgtggc gcgggaaccg gtcagtggtc tgcgccgcat cgtatgaagc ccggaagttt
    13681 ggtgtgcatt cggccatgcc tgcggtccgg gccgaacgcc tttgtccgga tgctgttttc
    13741 gtttcccctg atttcacccg ctaccgggct gtttcgcgcc aggtacggga aattcttcag
    13801 cgacacacgg atttgattga gcctctgtcg ctggatgagg cgtatctcga cgtgacggaa
    13861 aacaaaaccg gactgcctac ggcgacgcgt gtggccatag caatccgcaa gcagattcgg
    13921 gaagagttga atctgaccgc ctctgccgga gttgctccga acaagtttct ggccaagatt
    13981 gcctcggact ggaagaaacc ggacgggctt tttgtcatcc agccggagga agtggacagt
    14041 tttttgctac cactttctgt tgggcgcatt cccggagtcg gcaaggtgac gcagaagcac
    14101 ctggagcaga tcggggtgaa gacgattgcc caactacgaa gcctggagct ttccttgctg
    14161 gaagagcatt ttggccgtta tgggcaccgc ctttatgaac tggcgcgtgg gattgacctc
    14221 agtcccgtga tttctgaccg gccaacgaag tccatttccg cagaagacac cttcgagcgt
    14281 gacatgacgc tggacgagac aggagaaatc atccggcgtc ttgccgagag agtatggtcg
    14341 gcatcacgca aagatgccag gccagcacgt actgtggtgt tgaagctgaa gacggcggaa
    14401 ttccggattc tgacccgcag ccacacactt acaggtcctc ttgcttcctg cgaggagctg
    14461 acaagcatcg cgctctcact gcgcgaacgc gttggcttgg gtccggagca gcggtttcgg
    14521 ctggtggggg tgggactgca caattttcgt gatccggagg acccacagga acaaccggct
    14581 ttgttcgagg cttgaatgcg cgtctacata ataaaagcca tgccccggcc ttgagggccg
    14641 gggagatcga acattcatca gcaacggaca attttgggcg aggagatgcc ctttgtggcg
    14701 ttgcgggtat ctactaccaa ctgcgattcg cggacgatgc gctcgtagtc gtaatcagaa
    14761 tggtcggtgg cgatgaggac gcaatcgaat tgagacagat catcgagagg cgtgcagtgc
    14821 atgtccaggt tgtaatgacg gccgcggcct acatagggga agaagggatc gttgtaggag
    14881 acaatggcgc ctttctgacg gagcaat
//
LOCUS       DASRBV010000275         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_685302, whole genome shotgun sequence.
ACCESSION   DASRBV010000275 DASRBV010000000
VERSION     DASRBV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685302"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..111)
                     /locus_tag="VFA02_10150"
     tRNA            complement(35..111)
                     /locus_tag="VFA02_10150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Val,seq:gac)
     gene            272..688
                     /locus_tag="VFA02_10155"
     CDS             272..688
                     /locus_tag="VFA02_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HZU10252.1"
                     /translation="MASKVQVNARPEVKSSGFPYLALILGWLVPGLGHAVTKHWIRAL
                     LLFTSVTAMFVLGLAMQGKLYHANTGDTLEMLGFAGDLGSGLLYITGRIFDLGHGAIQ
                     LATADYGTKFIVVSGLLNVISAVDAHNLYIGRKPSC"
     gene            682..861
                     /locus_tag="VFA02_10160"
     CDS             682..861
                     /locus_tag="VFA02_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10253.1"
                     /translation="MLALSHFSSVLLFATFAAVVFGITQRTTPKAMFRYGLYCWTIFV
                     GSVIAASWLMWLIRR"
     gene            931..>2102
                     /locus_tag="VFA02_10165"
     CDS             931..>2102
                     /locus_tag="VFA02_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU10254.1"
                     /translation="MAFKFKGVDFIGFDALLSEDERLTRDTARQFIEDNLIPIIEECN
                     REGRFPRELVPQMGELGFFGANLHGYGCAGMSNVEYGLVMQEFERGDSGVRSFVSVQS
                     ALVMYPIYTFGSDEQKDYWLPLLAKGEKLGCFGLTEPDFGSNPGGMRTRARKVGDEYV
                     LNGEKMWITSGTISDVAVIWAKVDDEDGRVRGFLVETDRPGFSAYDVHGKWSLRASVT
                     SGLSLQDVHVPAKNLMPGTDGLKSALMCLNQARYGIGWGAIGAAMACYDTALQYSLVR
                     KQFRDQPIASHQLVQEKLTWMITEITKAQLLSLQVGRLKDAGKVEHQHISMAKRNNVW
                     MALECARLARDILGGNGITDDYPIMRHMMNLESVKTYEGTHDIHTLIIGQSVTGVPA"
BASE COUNT          427 a          585 c          600 g          490 t
ORIGIN      
        1 cggaattcga atgatcgtaa gttgttgaag gatatggtag gcacgagcgg attcgaaccg
       61 ctgacctcta ccgtgtcaag gtagcgctct aaccaactga gctacgcgcc tgcaaggtgg
      121 gttactgctc tcgttatgat atcaagtttt tgacctctgt gtgacgatga aaaccccggg
      181 ctgtttccgg cgtgatgggg ccataaatcc atacgaatga tatgtccgca cagcggcaac
      241 ggcggatttt tctgctgtat agtcattagt aatggcttcc aaagtccagg taaatgcaag
      301 gcccgaggta aagtcctccg gcttccccta tttggccctc atcttaggat ggctcgttcc
      361 cggtcttgga cacgcggtca cgaagcactg gattcgcgca ctgcttctct tcacctccgt
      421 tactgccatg tttgttctcg gcctggcgat gcagggcaaa ctgtatcacg cgaacaccgg
      481 tgacacactt gaaatgcttg gctttgccgg cgacctgggc agtggactgc tctacatcac
      541 cgggcgtatc ttcgacctgg gacacggagc catccagctt gccacggccg attacgggac
      601 aaagttcatc gtggtcagtg gccttctgaa cgtcatctca gcggttgacg cgcacaatct
      661 ctacattggc aggaagccct catgctagcg ctttcgcatt tctcttccgt cctgctcttt
      721 gcgacattcg ctgcggtggt cttcgggatc acgcagcgca cgacgccgaa agccatgttc
      781 cgctacggac tctactgctg gaccatcttt gttggatctg tcatcgcggc aagctggctg
      841 atgtggctta tccgccgtta gcttttgccc acttccgctt cgctctgtta gtctgactag
      901 tttcactttc agaacggagc aatcacatac atggccttca aattcaaggg cgtggacttc
      961 atcggcttcg atgcgcttct cagcgaagac gagcgtctga cgcgcgatac cgcccgccag
     1021 ttcattgaag acaatctcat tcccatcatt gaagaatgca accgcgaagg gcgctttccg
     1081 cgcgagcttg ttccccagat gggtgagctg ggctttttcg gcgcaaacct gcatgggtac
     1141 ggctgcgccg gcatgtccaa tgtggaatac ggcctggtga tgcaggagtt cgagcgcggc
     1201 gattccggtg tgcgcagctt cgtcagcgtg cagtcggcgt tggtcatgta tccgatttac
     1261 acctttggct ccgatgagca gaaggactac tggctccccc tgctggccaa aggtgaaaag
     1321 ctcggctgct ttggtttgac tgaacctgat ttcggctcga atccgggcgg catgaggaca
     1381 cgcgcccgca aagttggcga tgagtacgtg ctgaacggcg aaaagatgtg gatcacctcc
     1441 ggcacaattt ccgatgttgc cgtcatctgg gccaaagtag atgacgagga cggccgcgtg
     1501 cgcgggttcc tcgtggaaac cgaccgtccc ggtttttctg cttacgacgt ccacggaaaa
     1561 tggtcgctgc gtgcgtctgt tacgtcgggg ctttcgctgc aggatgtgca tgttccggcc
     1621 aaaaacctga tgcccggaac ggacggcctg aagtcggctc tgatgtgcct gaaccaagcg
     1681 cgctacggca tcggttgggg tgcgattggc gcggcgatgg cctgctatga cacggccctg
     1741 cagtattcgc tggtgcgcaa gcagttccgc gaccagccca ttgccagcca ccagctcgtt
     1801 caggaaaagc tgacgtggat gattaccgag atcaccaagg cccaactgct ttccctgcag
     1861 gtaggccgcc tgaaagatgc aggtaaggta gaacaccagc acatttccat ggccaagcgg
     1921 aataacgtgt ggatggccct ggaatgcgcc cggttggcgc gcgacatcct cggcggtaac
     1981 ggcatcacgg acgactaccc aatcatgcgg cacatgatga accttgaatc ggtgaagacc
     2041 tacgagggca cacatgatat tcacacgctc attattggac agagtgtgac gggggttccg
     2101 gc
//
LOCUS       DASRBV010000276         9911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_16966,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000276 DASRBV010000000
VERSION     DASRBV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16966"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..339)
                     /locus_tag="VFA02_10170"
     CDS             complement(1..339)
                     /locus_tag="VFA02_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZU10255.1"
                     /translation="MAEPTIHDQLDMEPTRHEHSVVSPKVYLGVFAALLCFTALTVAA
                     SYAELGIFNAVVALAIAVTKAVLVILFFMHIRYSSRLLKLTVASGIFTFLVLITLTLS
                     DYISRAWGLW"
     gene            complement(351..1100)
                     /locus_tag="VFA02_10175"
     CDS             complement(351..1100)
                     /locus_tag="VFA02_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HZU10256.1"
                     /translation="MDSQAIATQPTSAQEAHGEHGHSPHLRHHFATVAQQREAASFGM
                     WLFLLTEIMFFGGMFTAYLIYRNWYYPAFVAGSHWMVMWAGTLNTTVLICSSFTMAMG
                     VYSAETRNRKALLRYLTITLLFGLVFLLIKAYEWHDEWEHHHVPGINFSISDFTHPDP
                     RYPLDKPLPVDMAEKTQTYFSLYFMLTGVHALHMLVGVSILVVLLFQAWRGAYTTGHM
                     TTIENFGLYWHFVDIVWIFLFPLLYLISRHQ"
     gene            complement(1093..2757)
                     /locus_tag="VFA02_10180"
     CDS             complement(1093..2757)
                     /locus_tag="VFA02_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZU10257.1"
                     /translation="MSSTSIVLPDQQTAFLPKRSFLNSSYGLKSWLLTLDHKRIAILY
                     LITTCFFFTIGGTAAALIRLNLLTPGGELMAADTYNKVFSIHGIIMVFFFLVPIAPAV
                     LGNFCIPLMIGAKDVAFPRLNLLSWYIFVTGGLIELYAIIWGGIDTGWTFTTPLSTHY
                     VNTHVIAGAMGIFVAGFSSILTGVNFIVTIHRLRAPGMTWFRLPLFIWSYYATSIIFV
                     LATPVIAIALALVGLERFTGIGVFDPAKGGDPLLFQHLFWFYSHPAVYVMILPGMGIM
                     SEVVACFSRKRVFGYTAVAFSSVAIAVFSFFVWAHHMFIMGISNYSALVFSLMTMLVA
                     VPSAIKIFNWSSTLYKGSISFDTPILYAFSFIGLFTIGGLTGVYLASLGMDIHLTETY
                     FIVAHFHYVMVGGMVSALMAGLHFWWPKITGRMYPEMLGRLAACILFIGFNLTFFPQF
                     ILGYMGMPRRYHAYPPEFQVLNVLSTAGASILAIGYILPAIYFLWSLKYGKVAGANPW
                     RAPGLEWQVQSPPLEENFTETPVVDFEAYDFEWLAKQTSSEVQTVG"
     gene            complement(2754..3812)
                     /gene="coxB"
                     /locus_tag="VFA02_10185"
     CDS             complement(2754..3812)
                     /gene="coxB"
                     /locus_tag="VFA02_10185"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZU10258.1"
                     /translation="MMQYISPVLWQFLQKWLHRLPLFPPEASSIAPYTDEFYFYLLVI
                     SFIGTGIVVAMVIGFSVLYRKERNPEATQIEGNTLLEATWTIIPLGLFLIAFVWGALL
                     YFRIYNPPTNAMNIYIVGKQWMWKAEHPGGQHEINALHVPINRPVQLTMISQDVFHSF
                     SIPAFRVKREVIPGRYTTVWFEATQPGTYHLFCTQYCGTLHSQMIGEVVAMSPEDYQK
                     WTNSSTSGSSLAQNGERLFASLGCGQCHSGNPEARGPNLAGVYGTKIQLANGSFVTAD
                     DAFLRDIILNPTLHQVAGYAPIMPTYQGQVSEEGLISLVEFIKNLNSNYRIQQTLTTS
                     QVQSAAPTAAPGQGMVKQ"
     gene            complement(3819..4733)
                     /locus_tag="VFA02_10190"
     CDS             complement(3819..4733)
                     /locus_tag="VFA02_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZU10259.1"
                     /translation="MQRRTTIRRVGRISAIALALVVCLTGWLARAQVSGYGDKQMGDN
                     SDKLPPVLGRVRVVQKLNTRLPLEAEFRDETGKAVHLDDYFGRRPVILALVYYNCPML
                     CSEELNGLTGALEMVKFRPGKDFDVVVVSIDPTETYDLAAQKKAYYVKRYGHPETADG
                     WHFLTGQEPSIQALANATGFGYTRVPGPDGRMTQFAHASAIQLVTPDGRIAQYYLGVE
                     YSPKDIMLGLVEASQHKIGSPVDNILTYCYHYDPTLNRHSLIIARVVQLACLVTVFFV
                     GLYLLVAFRREAKQKQRQQLPNWKNVIG"
     gene            complement(4791..5471)
                     /locus_tag="VFA02_10195"
     CDS             complement(4791..5471)
                     /locus_tag="VFA02_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10260.1"
                     /translation="MPMHNDNLRKHSTSSGTHHANESRDMENSVREGYELTDVSVQGI
                     LVFLLALAVSLGVAFIFCFGMGKIVNNALVKHDGPPNKWTEMNYTGGGGKLRNMESNP
                     ALEQQQLKQLTQSFPTPRLQGDDGDRDTADLHAREDLLLENYSWVDRSKGTVRIPIER
                     AMELIAQRGLPVEQKPQQALPLMTGDHPIEVTAPLTDGFARTAYEQEMRSANREPGEQ
                     ASSNANNH"
     gene            complement(5458..6741)
                     /locus_tag="VFA02_10200"
     CDS             complement(5458..6741)
                     /locus_tag="VFA02_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10261.1"
                     /translation="MAQHAKTLPHELGAPEFVDRWRARALVIGAVFGALGIILGFLAG
                     DGWDHFLRAWLHGTMISFGFCCGGMALLMTQYLSGGKWGLLVRRPLEAMTRTLPLVFL
                     YFLPVGIFGMSFKKLYLWAQYNNDPWGAWKQHLISSTQAHSIYFKHPLLNPTTFWAVS
                     LFCFASWWLYTHLLNKWSLQRDADTAPNVPYWQVKFENLSGFGVVFYTLTLTAVSIYW
                     VMSLDVTWYSTVYGLQFLVGQAYGVLALVILTLIGLSKAEPIRTIMRVTEQHDLGKLC
                     FAFVMLNIYLAFAAFLIIWSGNSPEEIPWYLDRVRGNWGVIATLDVVFHWLIPFTLLL
                     SRDLKRIKGRLAVCCCVMIFARCWDMFWLIEPNFKDAARNLHFSIGILEYLTVPVAMI
                     AFWMAYYFTQLKARPLVATNDPHLEEILEPEHAHA"
     gene            complement(6751..7548)
                     /locus_tag="VFA02_10205"
     CDS             complement(6751..7548)
                     /locus_tag="VFA02_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU10262.1"
                     /translation="MDQIRIRRRKGLLAAGTLAALALVSGCRQDMHNQPKEIPQRGSE
                     FFTDGRSVRPQVVNTVARGQLHEDEYFYTGLLNGKEQDMMPFPVTMTVLERGQERFNI
                     YCTPCHSRVGNGDGMIVQRGYKPAGNFHDARRLAEPLSHYFYVMTNGYGAMPDYAAQL
                     SPADRWAVAAYIRALQLSQNAKLSDVPQGTQVHNLADIAERDGLPASFAGPWPMSEDT
                     NVQALPTQGIVAGAPAENANTINLTSKTEGNAKSTAKGAPATSGPKQ"
     gene            complement(7553..8098)
                     /locus_tag="VFA02_10210"
     CDS             complement(7553..8098)
                     /locus_tag="VFA02_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZU10263.1"
                     /translation="MSSVREGTYGLLAEFDSPGDLVRAAQAAYDSGYRRMDCYTPYPV
                     EEAASAIGFHRDEVSLVCLLGGLLGVAAMFSMETWISVWAYPLNIAGRPYYSWPAFIV
                     PAYEWTILWAGLSAFFGLFALCGLPEPYHPLFNAPNFRGGATSDKFFLCLEATDPKFD
                     VAQSRAFLEGFQPVSVVEVEY"
     gene            complement(8098..9519)
                     /gene="nrfD"
                     /locus_tag="VFA02_10215"
     CDS             complement(8098..9519)
                     /gene="nrfD"
                     /locus_tag="VFA02_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZU10264.1"
                     /translation="MAIQEPINDPMLDPVTGEIRVIAPGHTFKSVTEKVSRIVLTSHT
                     PVGWWGGLAIAGGGVLMLMVALTWLFLKGVGIWGITIPVAWGFAIVNFVWWIGIGHAG
                     TLISAILLLFKQSWRNSINRFAEAMTIFAVVCAGLFPLIHTGRPWLAYWLFPYPNTMN
                     VWPQFRSPLLWDVFAVSTYATISVVFWYLGMVPDLGTLRDRAQSKFAQFFYGIISLGW
                     RGSVRHWTRYETASLLLAGLSTPLVLSVHSVISFDFAVALLPGWHTTIFPPYFVAGAV
                     YSGFAMVITLAIPIRKFYHLEDLITIRHLDNMGKVMLVTGLIVAYGYWMEVFMAYWYS
                     ASRWEYFMMWNRMFGPMGWSYWVLITCNIALPQLMWARKFRRNPSLMFIMSFIVNTGM
                     WFERFVIVVTSLTRDYLPSSWGTYKATRWDYMTFAGTLGLFTFLFFLFIRFLPMIPMS
                     ELRILLPQAKIKAEPEPLPEAGD"
     gene            complement(9522..>9911)
                     /locus_tag="VFA02_10220"
     CDS             complement(9522..>9911)
                     /locus_tag="VFA02_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HZU10265.1"
                     /translation="KVRRFNFLLFSDYETESLKLMRNPDVTVRSRGVMEKCSYCIQRI
                     NAVKIEADKQNRDIRDGEIVTACQQACPTDAIIFGNINDRNSKVRKLKEQLRSYGVLA
                     GNNTRPRTTYVAEVLNINPELADGLEA"
BASE COUNT         2263 a         2948 c         2780 g         1920 t
ORIGIN      
        1 ctaccacagc ccccaggcgc ggctgatgta gtcgctcaga gtaagggtaa tcagaacaag
       61 aaaagtgaag atgccggaag ccaccgtcag cttcagcagg cgtgagctgt agcggatatg
      121 catgaagaac aggatgacga gcacggcctt tgtaacggcg atggccagcg cgacgacggc
      181 gttgaagata cccagttccg cataagatgc cgccacggtc aatgcggtga agcacaacag
      241 ggcggcgaag acacccagat acaccttcgg actgaccacg ctgtgctcgt ggcgtgtcgg
      301 ttccatatca agctggtcgt gaatggttgg ttctgccatg atgtcaatcc tcattggtgt
      361 cggctgatca gataaagcag ggggaacagg aatatccaga cgatgtccac aaaatgccag
      421 tacagcccga agttttctat cgtcgtcatg tgtccggttg tgtaggcccc tcgccatgcc
      481 tggaagagca ggacaacgag gatggaaact cccaccagca tgtgcaatgc gtggacgccg
      541 gtcaacatga agtagagcga aaagtaggtc tgggtcttct cagccatgtc tacaggcaga
      601 ggcttgtcca gtggatagcg cggatcagga tgagtgaagt cgctgatgct gaagttgatg
      661 cccggaacat ggtggtgctc ccactcgtcg tgccattcgt aggccttgat cagcaggaag
      721 accagtccga acagaagcgt gatggtcaga taccgcaaca gcgccttgcg attgcgggtc
      781 tccgccgagt agacacccat ggccatcgtg aagcttgagc agatgagcac agtcgtgttg
      841 agcgtgccgg cccacatcac catccagtgg ctgcccgcaa caaaggccgg gtaataccag
      901 ttccggtaga tgagataggc ggtgaacata ccgccgaaaa acatgatttc ggtcagcaga
      961 aagagccaca tgccgaagct cgcggcctcc cgctgctgcg ccaccgtggc aaaatggtgg
     1021 cgcaggtgcg gggaatgccc gtgctcgccg tgtgcttcct gcgcggacgt gggctgggtt
     1081 gcgatggcct ggctatccaa cggtctgcac ctcgctactg gtctgttttg ccagccactc
     1141 gaagtcatag gcctcgaagt caacgaccgg cgtttcggta aagttctctt ctaaaggagg
     1201 cgactgcacc tgccattcaa gcccgggcgc gcgccacggg ttcgcacctg caacctttcc
     1261 gtacttcagc gaccacagga aataaatagc aggcagaatg tagccaatgg caaggatcga
     1321 ggcaccggca gtggaaagta cattcagcac ctggaactcg ggtggatacg cgtggtagcg
     1381 ccgcggcatc cccatatagc caagaatgaa ctgcgggaaa aaggtcaggt tgaacccgat
     1441 aaacaggatg caggccgcca gtctccccag catctccggg tacatacgtc cggtgatttt
     1501 gggccaccag aaatgcagtc cggccatcag ggcactgacc atgccgccaa ccatcacata
     1561 gtggaagtgg gccacgataa agtacgtctc cgtcagatgg atgtccatgc ccagcgaggc
     1621 caggtagaca cctgtaagac cgccgatggt gaacagcccg ataaagctga acgcatacag
     1681 gatcggcgta tcaaagctga tggagccttt ataaagcgtg gacgaccagt tgaagatctt
     1741 gatggccgag ggcaccgcaa ccagcatcgt catcagagag aagacgagcg ccgagtagtt
     1801 cgagatgccc atgatgaaca tgtggtgggc ccacacaaag aagctgaaga ccgcaatcgc
     1861 cactgaagag aacgccacag cggtatatcc gaagacgcgc ttgcggctga agcacgccac
     1921 tacttcggac atgatgccca ttccgggcag aatcatgacg tagaccgccg ggtgggaata
     1981 gaaccagaac aggtgctgga agagcagcgg gtcgccacct ttcgccggat caaagacgcc
     2041 tatcccggtg aagcgctcca gacccaccag tgccagtgca atggcaatga ccggagtggc
     2101 caaaacaaaa atgatagatg tggcgtaata cgaccagatg aacagcggca gccggaacca
     2161 cgtcatgcct ggcgcgcgca gccggtgaat cgtgacgata aagttcacgc ccgtcaggat
     2221 ggacgagaac ccggccacaa agatgcccat tgccccggca atcacatgcg tgttcacata
     2281 atgcgtgctc agcggcgtgg tgaaggtcca gcccgtatca atgccgcccc agatgatggc
     2341 gtacagctcg atcaggccgc cagtaacgaa tatataccag ctcagcagat tcaatcgcgg
     2401 aaaggccacg tccttggcgc caatcatcag cggaatgcag aaatttccca gcaccgccgg
     2461 agcaatcggc actagaaaga aaaacaccat gatgatgccg tggatggaga acaccttgtt
     2521 ataggtgtct gcggccatca gctcgccgcc gggggtcagc aggtttaggc ggatgagcgc
     2581 cgccgccgtg cctccgatgg tgaagaagaa gcatgtagta atcagataca ggatggcaat
     2641 acgtttatgg tccagcgtca gcagccagct cttcagccca taagagctgt tcagaaatga
     2701 acgcttcggc aaaaaggccg tctgctgatc gggaagaaca atacttgtgc tgctcattgc
     2761 ttcaccattc cttgtccggg tgcagcggtc ggtgctgcgc tctggacctg gcttgtcgtg
     2821 agtgtttgct gaatacggta gttcgagttc aggttcttaa tgaattccac cagggaaatc
     2881 agaccctctt cactgacctg cccctggtag gtcggcataa tcggcgcata cccggccacc
     2941 tggtgtagcg tcgggttcag gatgatgtca cgcagaaacg catcatcggc ggtcacaaaa
     3001 gagccgttgg ccagttgtat ctttgttcca tagacccctg ccaggttcgg cccgcgcgct
     3061 tcagggtttc ctgagtggca ctggccgcag cccaggctgg caaacagcct ttcgccgttc
     3121 tgcgccagcg aagagccgct ggtcgagctg tttgtccact tctgataatc ttccggcgac
     3181 atcgccacaa cttctccgat catctgcgag tgcagggtgc cgcagtactg cgtgcagaac
     3241 agatgatagg tcccaggctg ggtcgcctca aaccacacag tcgtgtagcg gcccggaatg
     3301 acctcccgct tcacgcggaa ggcggggatg gaaaagctgt ggaagacgtc ctgcgagatc
     3361 attgtcagtt gcacagggcg gttgatgggc acatgcagcg cgttgatctc atgctgtccg
     3421 cccggatgct ccgccttcca catccactgc ttgccgacaa tgtagatgtt catggcgttg
     3481 gtcggcggat tgtagatgcg gaagtacagc agcgcgcccc atacaaaggc aatcaaaaac
     3541 aggcccagcg gaatgatggt ccaggtggct tccagcagcg tgttgccctc aatctgcgtg
     3601 gcctccgggt tcctctcctt gcggtacagc acggagaagc caatcaccat cgccaccacg
     3661 atgccggtgc caatgaacga aatcacaagc agataaaaat agaactcgtc cgtatagggc
     3721 gcgatgctcg acgcctcggg cggaaacagc gggagccgat ggagccactt ctgtaagaac
     3781 tgccaaagta cggggctaat gtattgcatc atcccttttt atccgatcac gtttttccag
     3841 tttggaagct gctggcgctg cttttgcttg gcttcgcgcc ggaacgccac taacaaatac
     3901 aaaccaacaa aaaatacggt cacaagacag gccaactgca ccacacgtgc aatgatcagg
     3961 ctgtgcctgt tcaacgtcgg gtcatagtgg tagcagtagg tcaggatgtt gtccaccgga
     4021 gagccaatct tgtgctgcga tgcctccacc aggcccagca tgatgtcctt cggcgaatac
     4081 tccaccccga ggtaatactg ggcaatgcgt ccgtccggcg tgactagttg aatggcgctg
     4141 gcgtgggcaa actgcgtcat tcttccgtcc ggacccggca cgcgcgtgta accaaatcct
     4201 gtggcattgg ccagggcctg aatggagggc tcctgtccgg taagaaaatg ccagccgtct
     4261 gccgtctccg gatgcccgta gcgctttacg taatacgcct tcttctgcgc cgccagatcg
     4321 taggtttcag ttggatcaat gctgactaca acaacatcaa agtccttgcc cgggcgaaac
     4381 ttcaccattt cgagcgcgcc tgtcaggccg ttgagctcct ccgagcacag catggggcag
     4441 ttgtagtaga ccagtgccag aataacgggc cgtctgccga aataatcgtc cagatggacc
     4501 gcctttcccg tctcatcgcg gaactccgct tccagcggca ggcgggtgtt cagcttctgc
     4561 acgacacgca cccggcccag caccggaggc agcttgtcgc tgttgtctcc catctgcttg
     4621 tcaccatagc cggagacctg cgcgcgggcc agccatccgg tcagacacac aacgagcgca
     4681 agcgcgattg cgctgatacg cccaactctc cggattgttg ttctacgctg catatgtctt
     4741 tatgctagtc tgctggccgg ctctgtttcc ggccccttta agcccctgct ttaatgattg
     4801 ttcgcattac tggaagcctg ttcacccggc tccctgtttg cactgcgcat ctcttgctca
     4861 taggccgtcc gtgcaaagcc atcggtcagc ggggcagtca cttctatggg atggtcacct
     4921 gtcatcagcg gcaacgcttg ctggggcttc tgttccacag gcagcccgcg ctgggcaatc
     4981 aactccatgg cccgctcaat cgggatacgg accgtgccct tcgagcggtc cacccaactg
     5041 tagttttcca gcaacaggtc ctcgcgcgca tgcagatcgg ccgtgtcccg gtcgccatca
     5101 tcgccctgga gccgcggcgt cgggaatgat tgtgtcaact gcttgagctg ctgctgttca
     5161 agcgccgggt tcgactccat attgcgcagc tttcctccgc ctccggtgta attcatctcc
     5221 gtccatttgt tcggaggtcc gtcatgcttc accagggcgt tgttgacgat cttgcccatt
     5281 ccgaagcaga aaatgaaggc caccccgagc gagacggcca gggccagcag aaagaccaga
     5341 atgccctgca cgctgacatc ggtcagctca tagccttcgc ggaccgagtt ctccatatca
     5401 cgcgattcat tcgcgtggtg cgttccgctg ctcgtgctgt gtttccgcag attgtcgtta
     5461 tgcatgggca tgttcaggct ccagaatctc ttcgagatgc ggatcgtttg tcgccaccag
     5521 cggacgcgct ttcagttgcg tgaagtagta cgccatccag aaggcaatca tcgccaccgg
     5581 gaccgtcagg tactccagaa tgccgatgct gaagtgcagg ttccgcgccg cgtctttgaa
     5641 gttcggctca atcagccaga acatgtccca gcaacgggca aaaatcatca cgcagcagca
     5701 gaccgccagc cgtcccttga tccgcttcag gtcgcgcgag agcagcagcg taaacggaat
     5761 cagccagtgg aagaccacgt ccagcgtcgc gataacgccc cagttgccgc gcacgcggtc
     5821 caggtaccag ggaatctctt ccggtgagtt ccctgaccag ataatcagaa acgcagcaaa
     5881 cgccagatag atgttgagca tcacgaaggc aaagcacagc ttgccgaggt catgctgctc
     5941 ggtcacgcgc atgatggtcc tgatcggctc cgctttggac agcccaatca gggtcaggat
     6001 caccagcgcg agcaccccat acgcctgccc gaccagaaac tgcaggccat agaccgttga
     6061 gtaccacgtc acatcgagcg acatgaccca gtagatcgag accgctgtga gcgtaagcgt
     6121 gtaaaacacc acgccgaagc cgctcaggtt ttcaaacttt acctgccagt aaggaacatt
     6181 tggcgctgtg tccgcatcgc gctggagcga ccacttgttc aacagatggg tatacagcca
     6241 ccagctggca aagcagaaca gcgataccgc ccagaacgtc gtgggattca gcagaggatg
     6301 cttgaaatag atactgtgcg cctgcgtcga actgatgagg tgctgcttcc atgcgcccca
     6361 tggatcattg ttatattgcg cccacaggta cagcttcttg aaggacattc caaagatgcc
     6421 gacaggcaga aagtacagaa agaccagtgg cagtgtgcgg gtcatcgcct ccagcggacg
     6481 ccgcaccaga agcccccact tgccgccgga aagatactgc gtcatcagca gcgccattcc
     6541 gccgcagcag aagccgaacg agatcatcgt cccatgcagc catgcgcgca gaaagtggtc
     6601 ccagccatca cctgcgagga acccaaggat gattccgagc gcgccgaaga ccgcgccaat
     6661 caccagcgcc cgtgcgcgcc agcggtccac aaattccgga gcgcccagtt cgtgcggcaa
     6721 tgtttttgcg tgttgcgcca tcgtcattcc ttattgcttt ggcccggatg ttgccggagc
     6781 gcctttggca gtgctcttcg cattgccctc tgttttgctt gtcagattga tcgtattcgc
     6841 attctccgcc ggagcacccg caactatgcc ctgcgtcggc agcgcctgca catttgtgtc
     6901 ctctgacatc ggccacggcc cggcaaaact ggccggcagt ccgtcccgct cggcaatgtc
     6961 cgccagatta tgcacctgcg tgccctgagg aacgtccgac aacttcgcat tctggctcag
     7021 ttgcagcgcc cggatatacg ccgccaccgc ccagcggtct gccggtgaaa gctgtgccgc
     7081 gtagtccggc atcgccccgt agccattggt cattacatag aaatagtggc tcagcggctc
     7141 ggccagtcgt cgcgcatcat ggaagttgcc cgcaggttta tatccgcgct gcacaatcat
     7201 gccgtcgcca ttgcccacgc gcgagtggca cggcgtgcaa tagatgttga agcgctcctg
     7261 cccgcgttcg agtacggtca tggtcaccgg gaacggcatc atgtcctgct ccttgccgtt
     7321 cagcaatccg gtgtagaagt actcatcctc gtgcaactgc ccgcgcgcca ctgtattgac
     7381 cacctgcggc cgcaccgatc ggccatcggt gaaaaattca gacccgcgct gtggaatctc
     7441 tttgggctgg ttgtgcatgt cctgacggca gcctgaaacc agcgccagcg ccgccagcgt
     7501 cccggctgcc agtagccctt tccgtctcct gatgcggatt tgatccatgg tcttaatact
     7561 ccacctccac tacggagacg ggctgaaaac cttcaagaaa tgctctggac tgggccacat
     7621 caaacttagg gtccgtggcc tccaggcaaa gaaaaaactt gtccgaggtc gccccaccgc
     7681 ggaagttcgg cgcattgaac agcggatgat acggttccgg caacccgcac agggcaaaaa
     7741 gcccgaaaaa cgccgagagg cccgcccaca ggatcgtcca ctcataagca ggaacgatga
     7801 acgcaggcca tgagtagtaa ggccgtccgg caatgttcag cggataggcc cacacggaaa
     7861 tccaggtctc catgctgaac atggctgcga cccccagcag gccgcccagc agacagacca
     7921 gcgacacctc atcgcggtga aagccaatcg ccgacgccgc ctcttccact gggtacggcg
     7981 tgtagcagtc catgcggcgg tatccggaat cgtaagcggc ctgcgcggcc cgcaccaggt
     8041 cgcccggaga atcgaactcc gccagcagtc cgtaagtccc ttcacgtaca gaagacatca
     8101 gtcacctgcc tccggcaaag gttctggctc cgccttgatc tttgcctggg gcagcagaat
     8161 tcgcagctca gacatcggga tcataggcag gaagcggata aacaggaaga acaggaaagt
     8221 aaacaggccg agtgtcccgg caaaggtcat gtagtcccag cgcgtggcct tgtaagtgcc
     8281 ccacgaggac ggcagataat cgcgcgtcag gctggtgaca acaatcacga aacgctcaaa
     8341 ccacatgccc gtgttcacaa tgaacgacat gatgaacatg agcgagggat tgcgccggaa
     8401 cttcctggcc cacatcagtt gcggcagcgc aatgttgcag gtgatgagca cccagtagga
     8461 ccagcccatc ggcccgaaca tgcggttcca catcatgaag tattcccacc ggctggccga
     8521 gtaccagtag gccatgaaga cttccatcca gtagccatag gccacaatca gcccggtcac
     8581 cagcatgacc ttgcccatgt tgtccaggtg gcggatcgtg atcaggtctt caaggtgata
     8641 aaacttgcgg atgggaatgg ccagcgtgat caccatcgca aagccggaat acacggcgcc
     8701 agccacgaaa tatggcggga aaatcgtcgt atgccaaccc ggaagcagcg ccacagcgaa
     8761 atcaaagctg atgacgctat gcacggagag caccagaggc gttgaaagcc cggccagcag
     8821 cagtgaggcc gtctcatacc gtgtccagtg ccgcaccgag ccgcgccatc ccagcgagat
     8881 gattccgtag aagaactgtg caaacttcga ttgcgcacgg tcacgcagcg tacccaggtc
     8941 cggcaccatg cccagatacc agaagaccac cgaaatcgtt gcgtaggtcg agaccgcaaa
     9001 aacgtcccac aacagtggcg aacgaaactg cggccacaca ttcatcgtgt tgggataagg
     9061 aaaaagccag tacgccagcc acggacggcc cgtgtgaatc agcggaaaca gcccggcgca
     9121 gaccacagcg aagatcgtca tcgcctcagc aaaacggttg atggagttgc gccagctttg
     9181 cttgaagagc agcagaatgg ctgagatcag cgtccctgcg tggccaatac cgatccacca
     9241 cacaaaattg acgatggcaa agccccacgc caccggaatt gtgatgcccc agatgcctac
     9301 acctttcaga aacagccagg ttagggccac catgagcatg agcacgccgc ccccggcaat
     9361 ggccaggccg ccccaccatc ccacaggagt gtggctggtc aggacaatgc gcgagacctt
     9421 ctcggtgacc gacttgaagg tgtgtccggg cgcaatgacc cggatttccc cagtcaccgg
     9481 gtccagcatt ggatcgttga ttggttcctg gatagccatg tttaagcctc aagcccatcg
     9541 gccagttcag ggttgatgtt cagtacctcc gccacatagg tcgtgcgcgg acgcgtattg
     9601 ttgcccgcca gcaccccgta gctgcgcaac tgctctttca gtttgcggac cttgctgttg
     9661 cggtcattga tgttgccgaa gatgatcgcg tccgtcgggc aggcctgctg gcacgctgtc
     9721 acaatctctc catcgcggat atcgcggttc tgcttgtccg cctcaatctt caccgcattg
     9781 atgcgctgga tgcagtagct gcacttttcc atcacgccgc ggctgcgcac cgtcacatca
     9841 ggattgcgca tcagcttcag gctctccgtc tcataatcgg agaagagcag gaagttgaag
     9901 cggcgcacct t
//
LOCUS       DASRBV010000277         5316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_16985,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000277 DASRBV010000000
VERSION     DASRBV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16985"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2637
                     /locus_tag="VFA02_10225"
     CDS             <1..2637
                     /locus_tag="VFA02_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10266.1"
                     /translation="YNMEWRSLIQGNNFNVGVPFTSTYGGDLSQAINTGIVTSVRAPF
                     DCQVSQAVSSRFAAAGQPLSHCGPGGTTDPSKGAVIVPFNNNTIPASLLDPNAQTLLK
                     AGIFPAPNTGARFVGPANAPTNVREEIVRIDHTFSQKFSIFGHYIAEQVSQTDIPTRW
                     SSANLPTVGDTFGNPSYSAVVGATYAINSNMLNEARFTYGGNRINILPIGISNVPGFQ
                     QNRIFNGTVSVTPTISLGSQTGATYNNNWEPWQNTADSYGISDDFSWLKGNHQFKVGG
                     SWLYFLKAQPLQVSPQGSFTFNGAFTGYDFADFLLGLTQSYSEAALKDTRHWNSMSYA
                     LYFQDDWHALPRLTLNLGLRWDGIPHTYEANKQHANFYPNLYNPAEAPAFDPTSGDNA
                     ILLSSPGLGPSPNPVLQGYQFYLNGIGIGGVTSGVPRGLVKNHWLAFGPRIGFAYDVT
                     GTGNTVFRGGFGIMYERIQGNDMYQSGANVPFSANINLNNVSLSDPHVRVDTGQVINA
                     QQLPVVVPSITALNSDKYRLPMSMQYSLGFQHALTRQSVLNVSYVGNVDRFQSDAVNI
                     NLPSYSMLPCLVGRSNCPSSNTTYNGSLPYLGYHSILMDQNESSSNYNSLQASLNSNF
                     TSMGLQLQLGYTYSKAMDATTGTGGDGFDLDQVSNPYIGWTYDMGPSVFDRTHVGFAD
                     FIYDLPFFRNSSSSLLKSTIGGWQLSGIVNMMTGAPLNLGVNNGGPVSNNICNVVSNC
                     SIRPNVNGRITYPKSHTTLSSGNNSVQWFDPAPFSPAILPGTSSIATFGNLSHNALRG
                     PGRDNWNLALFKQFPLWREQQHIELRAESYNVFNHTQFSANTVGGGASGGIGSSLYGT
                     DFGKINSAYDPRTFQFGAKIVW"
     gene            2697..3824
                     /locus_tag="VFA02_10230"
     CDS             2697..3824
                     /locus_tag="VFA02_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10267.1"
                     /translation="MLFRLVFCAIVLFYSVVQAQSVTPSTAMALEEQGKLAEASAAWQ
                     QITKQHPTDAAAFASLGLVLAKQGRFTEAVPAYKKAVALNPKLPGLQLNLGLAEFKQG
                     HFREAVSPFTAALTENPHSLQARVLLGMSFYGSEQYAKASEFLADAVKADPENLQLRQ
                     TLAQSCLFAKKYSCALDEFQQILQKDPDSAAVHILYGEALDGLDRTPEAIAEFQAAAK
                     AAPQEPNVHFGLGYLYWKLLKFDQAKQEFERELALYPDQPQALAYLGDVEWKNGNQER
                     AQELLEKAVHLRNIRIAWLDLGAIFQQEHRYPDAVTALKRAIELDPSQPEAYFLLGRV
                     YQAMGDKEEAQKQFAKTRTLQKGQLDKLATQMPRPAMSPLQ"
     gene            complement(3826..4986)
                     /locus_tag="VFA02_10235"
     CDS             complement(3826..4986)
                     /locus_tag="VFA02_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10268.1"
                     /translation="MQNWTTKAVIAAVLCICTPPSWAQDDAQSLSEQGREALQAGRYP
                     DAERAYQQLERLQPEAAEIHATLGVVYFKEGKFDRAVTELRRAQQLKPSLPQVEALLA
                     MSLSEQGEFQQALPELDKCFHKTADPQLRKMCGLRLERAYVSLHDDTKAVETALALQQ
                     AYSEDPEVLYYSSKVFGNEAFLAAQKLFQTSPDSVWGLMAAGEAHESQGDIEEAIRYY
                     REVLNLDPAKPNIHFRIGRALLKVGPQHSAEAAAEFQQELQMDPANANAAYELAEIYR
                     EQGEEEQARKYFQQALDHWPDFEEAHVGLAATVMQRNPEVAQQHLRKAIALDPSDSVA
                     WYRLAQVERALGHSEEQKYALAKFTKLKNQGTDHASFHPEVTPQHLDPETGP"
     gene            complement(5050..>5316)
                     /locus_tag="VFA02_10240"
     CDS             complement(5050..>5316)
                     /locus_tag="VFA02_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10269.1"
                     /translation="NNWDMGLAKNFAFGERVNFQLRLESFNTFNHTQWGVDPSSPTAA
                     ASGPGTGAIDRNVNDQPPSANTNFGRVVSARPGRILQLGGKITF"
BASE COUNT         1216 a         1480 c         1376 g         1244 t
ORIGIN      
        1 tacaacatgg aatggcgcag cctgatccag ggcaacaact tcaatgttgg tgtccctttt
       61 accagcacct atggcgggga cctttcgcag gccatcaata caggaatcgt aacgagcgtg
      121 cgtgcacctt ttgattgcca ggtctctcag gcggtcagca gcaggtttgc tgccgctggc
      181 caaccgctca gccattgcgg gccgggagga acaacggacc ccagcaaggg ggctgtgatt
      241 gtgcccttca acaacaacac gattcctgcc tcgctgcttg atccgaatgc gcagaccttg
      301 ctgaaggcgg gtatctttcc tgcgccgaat actggggcgc gcttcgtagg tccggccaat
      361 gctccgacaa atgtccggga agagattgtc cgtatagacc acacctttag ccagaaattt
      421 tccatcttcg gacactacat cgcagagcag gtttcccaga cggacattcc gacccgctgg
      481 agcagcgcca accttccgac cgtcggtgat acctttggaa atccttcgta cagcgcggtt
      541 gtaggtgcaa cgtacgccat caacagcaat atgctgaatg aagcgcgctt cacctatggt
      601 ggaaaccgga tcaacatcct gccgattggt atcagcaatg ttcccgggtt ccagcagaac
      661 cgtatcttca acggaacggt cagtgttacc ccgaccatca gcctcggcag tcagacgggc
      721 gcaacctaca acaacaactg ggaaccgtgg caaaacactg cggattccta cgggatttcc
      781 gacgattttt cgtggctcaa gggaaaccac caattcaagg ttggcggaag ctggttatat
      841 ttcctgaagg cccagccttt gcaggtttca ccgcaaggaa gctttacatt taacggagca
      901 tttacaggct atgactttgc tgacttcctg ctcggattga cgcaaagcta cagcgaggct
      961 gctctgaaag acacccgcca ctggaacagc atgtcctatg cgctgtattt ccaggacgat
     1021 tggcacgctc tgcccaggct tacgctgaat ctgggcctgc gctgggacgg cattccgcac
     1081 acgtatgaag ccaacaagca gcatgcgaat ttttatccca atctctacaa ccctgctgag
     1141 gcaccggcct ttgatccaac cagcggggac aatgcaattt tgttgagcag cccgggacta
     1201 ggccccagtc cgaatccggt attgcaaggc tatcagttct acctgaacgg catcggcatt
     1261 ggcggagtca cgtccggtgt tcccagaggt ctggtaaaaa atcactggct ggcttttggc
     1321 ccgcgcatcg gctttgccta tgatgtcacc ggcacaggca atacggtatt ccgcggcggc
     1381 ttcggtatta tgtatgagcg catccagggc aatgacatgt atcaatccgg cgcgaacgtg
     1441 cccttcagcg caaacatcaa cctcaacaac gtttcacttt ctgacccgca tgtgagagtg
     1501 gacactggac aggtcatcaa tgcgcagcaa ttgcccgttg tcgttccctc cattacggcg
     1561 ctcaattctg acaagtaccg tctgccaatg agcatgcagt atagtctggg attccagcat
     1621 gcgttgacac gccagtccgt gctcaacgtg tcctatgtcg gaaatgtgga tcgcttccag
     1681 agcgatgcgg tcaacattaa tctcccgtcc tacagcatgc ttccgtgtct tgtagggaga
     1741 agcaactgcc ccagctccaa cactacatat aacggttcgc tcccgtactt gggatatcac
     1801 tctatcctga tggaccaaaa cgaatcgagc tccaactata actcgctgca ggcgtcgctt
     1861 aattccaact tcaccagtat ggggctgcaa ttacaattgg gttacaccta ctccaaagcg
     1921 atggacgcca ccaccgggac cggaggcgat ggttttgatc tcgaccaggt ctcgaatcca
     1981 tatatcggat ggacctatga tatgggcccc tccgtctttg accgcaccca tgtgggcttt
     2041 gccgatttca tctatgatct gcccttcttc cgcaactcgt cgagttcgct gctgaagtca
     2101 acgatcggag gctggcagct ctctggcatt gtcaatatga tgacaggtgc tccgctcaac
     2161 cttggagtga acaatggcgg ccctgtctcg aacaatatct gcaacgttgt ttcgaactgc
     2221 tccatccggc ccaatgtgaa cggacgcatc acttatccaa aatcacacac gacgctctcc
     2281 agcggcaaca actctgtgca gtggtttgat ccggccccgt tctcaccggc gatcctgccc
     2341 gggacctcga gcatagcgac atttggcaat ctcagccaca atgcgctgcg cgggcccgga
     2401 cgcgacaact ggaacctggc gctgttcaag cagttcccac tctggcgcga acagcagcat
     2461 atcgaactgc gggcagagag ctacaacgtc tttaaccaca cgcagttctc tgcaaataca
     2521 gtgggcggag gcgcgagcgg tggcatcggc agctcactct atggcacgga cttcggcaag
     2581 atcaattcgg cctacgaccc gaggaccttc cagttcgggg ccaagatcgt ctggtagttt
     2641 gtcttttctt caaccgctct tcgccaacca gcgaagggcg gttttatttt atgtgtatgc
     2701 tctttcgctt agtattttgc gccattgtgc tgttctacag cgtcgttcag gcgcaatccg
     2761 ttactccttc aactgcaatg gcgcttgaag agcagggaaa gctcgccgaa gctagcgccg
     2821 catggcagca gattacaaaa cagcatccca cagatgcggc cgcctttgcc agcctggggc
     2881 ttgttctcgc aaagcaaggc aggtttacgg aagctgttcc cgcttacaaa aaagccgtgg
     2941 cgctcaatcc caaattgcca ggattgcaac tgaatcttgg actggccgaa ttcaagcaag
     3001 gccactttcg agaggcagtc tcgccgttta cggcagctct gactgaaaat ccgcatagtc
     3061 tgcaggcacg cgtgctgctg ggtatgagct tttatggctc agaacaatat gccaaagcat
     3121 cagaatttct ggcggatgcc gtcaaggccg atcccgaaaa cctgcaattg cggcagacgt
     3181 tggcgcaaag ctgtcttttc gcaaaaaaat actcgtgcgc attagacgaa tttcagcaaa
     3241 tactgcaaaa agaccctgat tcagcggcgg tacacatcct gtatggagaa gcgctggatg
     3301 gcctcgaccg gactccggag gccattgctg aatttcaggc agcagcaaag gccgctccac
     3361 aggagccgaa tgtgcacttt ggcctcggct atctgtattg gaagctgctc aagttcgatc
     3421 aggcgaagca ggaatttgaa cgtgaactcg cgctctatcc agaccagccg caggcattgg
     3481 catatctcgg cgacgtggaa tggaagaacg gcaatcagga aagggcacaa gagctgctgg
     3541 aaaaggccgt gcatctcaga aacattcgca ttgcatggct tgatctcggc gcgatttttc
     3601 aacaagagca ccgctatccc gatgcagtca ctgctttgaa acgagccata gaactggacc
     3661 cctcgcagcc tgaagcatac tttctgttag ggcgtgtcta tcaggcgatg ggagacaagg
     3721 aagaagcgca gaaacaattt gcaaaaacac gaacgctgca gaaagggcaa ttggacaagc
     3781 ttgccaccca gatgcctcgg ccagctatgt ctcccttgca gtagtttatg gaccagtttc
     3841 cgggtcgagg tgttgtggcg tgacttcagg atggaaagat gcgtggtccg taccctggtt
     3901 cttcagcttt gtaaatttcg ccagcgcgta tttttgttcc tcagaatgcc ccagtgcgcg
     3961 ttcgacctgc gccagcctgt accatgcgac agagtcactg ggatcaagag caatggcttt
     4021 ccgcaaatgc tgctgtgcga cctccggatt gcgctgcatc acagttgcag caagaccaac
     4081 atgggcctct tcaaaatcag gccagtggtc taacgcctgt tggaagtatt tccgggcctg
     4141 ttcttcttct ccttgctcgc ggtagatttc agcgagttcg tacgctgcat tcgcatttgc
     4201 ggggtccatc tgaagctctt gttggaactc ggctgctgct tcagcagagt gctgggggcc
     4261 aactttcagc agtgctcttc caatacggaa atggatgttg ggctttgctg ggtcgagatt
     4321 cagcacttca cggtaatagc gaatggcctc ttctatatcg ccctgacttt catgggcctc
     4381 tcctgcggcc atcaatcccc agacagagtc gggagaggtc tgaaacagct tctgcgctgc
     4441 gagaaatgct tcgttgccga agaccttgct cgaataatac aggacttcag gatcttcgga
     4501 atatgcttgc tgcaaggcaa gagcagtctc cacagctttt gtatcgtcgt gcagagacac
     4561 ataggcacgt tccagacgaa gaccgcacat ttttcttagc tgagggtctg cagtcttgtg
     4621 aaaacattta tcgagctccg gcaatgcttg ctggaactct ccctgctcgg aaagcgacat
     4681 agcgaggagc gcttccacct gtggcagaga tggttttaat tgctgtgccc gccgcagttc
     4741 tgtaacggcc cggtcaaatt ttccttcctt aaaatagacc acgccgagag tggcatggat
     4801 ttctgccgct tcaggctgta gtctttccag ttgctgatag gcgcgttctg catcaggata
     4861 tctgcctgcc tgaagggcct ctcttccttg ttctgacaaa gattgcgcat cgtcctgcgc
     4921 ccaggatgga ggtgtgcaaa tacacaacac cgcagctatg accgcttttg ttgtccagtt
     4981 ctgcatggct gcgagtctat ctccaaatga aaagggcctg ccatcacgag cgacaggccc
     5041 ttattcaact caaaaagtaa tcttacctcc taattggaga atgcgcccgg gccgtgctga
     5101 gaccacacgc ccaaagttag tgtttgcgct tggcggttga tcgtttacgt tgcggtctat
     5161 ggcgccggtc ccaggaccac tggctgcggc agtgggcgag cttgggtcca ctccccattg
     5221 cgtgtgattg aaggtgttaa acgactcaag acgaagctga aaattcaccc tttctccaaa
     5281 ggcaaaattc ttggccaacc ccatgtccca gttgtt
//
LOCUS       DASRBV010000278        17418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_272121, whole genome shotgun sequence.
ACCESSION   DASRBV010000278 DASRBV010000000
VERSION     DASRBV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 17418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17418
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272121"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFA02_10245"
     CDS             complement(<1..263)
                     /locus_tag="VFA02_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZU10270.1"
                     /translation="MATTATPTPAPKVNRLGLPILEYRGGKTTLCAGCGHNAISERII
                     DAMYEMGVQPERVMKLSGIGCSSKSPAYFLSRAHSFNSVHGRMP"
     gene            complement(266..2116)
                     /locus_tag="VFA02_10250"
     CDS             complement(266..2116)
                     /locus_tag="VFA02_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZU10271.1"
                     /translation="MATGDVALGQQAAGALARDASKRIVNDFSIQVATVNGSGSQSAN
                     NVLLRSIFAMGIPVSGKNLFPSNIAGLPTWYTIRASKYGYIARKKEIDILVAMNPETA
                     KEDILSLPAGAVAIYEEALNLKQYRDDVTCYPVPFDKLTAAVCPEARLRKLVKNMIYV
                     GVVAQLLSLDMVAVEAALRRQFAKKQKAADLNWAAVQAGYDFASQSLTKQDPFVLERM
                     HETDGKIIIDGNAASALGAMFAGVTVVTWYPITPSSSVVEQLIDYLKKYRVEPDGKAS
                     FAVVQAEDELAAVGMVLGAGWAGARSMTATSGPGISLMAEFAGLGYFAELPGVIFDIQ
                     RVGPSTGLPTRTAQGDILSIAYLSHGDTKHIMLLPGNVKECFEFSIAAFDLAERFQTP
                     VFVVSDLDLGMNNWMSEPFEYPEKPLDRGKVLTAEDLKRLGEFARYKDVDGDGIPYRT
                     LPGTDHPKAAYFTRGSGHNEKALYTERPDDYQNLMERLHRKFETARTQVPAPVVLQTG
                     KSKVGIIAYGTSDFAVQESRDQIRKEYGVDTDYVRIRAFPFTKDIHEFVASHDRVYIV
                     EQNRDAQMLSLLKLDLPAEDAVKLRSIRHFNGLPIDARSITDEFVLQEGI"
     gene            complement(2403..3458)
                     /locus_tag="VFA02_10255"
     CDS             complement(2403..3458)
                     /locus_tag="VFA02_10255"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZU10272.1"
                     /translation="MLLEGIFPAITTPFHTDGGLYLRKLEMNVERYSRAPVSGLVVLG
                     STGEAVMLSDEESREVLRTARAAASPQKVLLAGVGRESAVATLKLAEFAAGQQYDAVL
                     VRTPSYYSPQMQVLEMLTYFRMVADRSPLPVVLYSIPKFTKYELSIELVAELSQHPNI
                     IGIKDSSGSIERVAQLVSVTRNAPRRTTVVTNVFTAFTNRMLQEQALADTTFVPADVL
                     GLGAPAAIAPPGPKIATRTREVGFQVLSGSPDNVKEALDTGASGAVLAFGACAPQACQ
                     EIYMAWKDNDPKLAQLKQERIKEASRIVAGKFGVPGIKFACDLNGYYGGVPRVPLLPL
                     KAEEQSVVTKAMEDVRN"
     gene            complement(3468..4217)
                     /locus_tag="VFA02_10260"
     CDS             complement(3468..4217)
                     /locus_tag="VFA02_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10273.1"
                     /translation="MRLLRNLCLAVTGIAALLAYSQSSPIDEGLAALKSHQPQQALAA
                     FQQALQANPNDVAANLYAAGAALELYQGNLAVQYAEKAKQLAPDNWKVHTTLVVAYAM
                     AGKIQERDAERELLRKLHADPKAPEAMQTNGFLLDLFPVKQYRIEAIEFFKPVGKFNV
                     YYRFHVRNTQGKLVWSFNVESNDFDEKSWEQAHAQQAADGERQFQITGEGNGKSVDYD
                     MFSGRPAYDSIKARILRILNAQTAPFPGETT"
     gene            4372..5604
                     /locus_tag="VFA02_10265"
     CDS             4372..5604
                     /locus_tag="VFA02_10265"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZU10274.1"
                     /translation="MKKHTVDPLAVHRNAIVIDTHADTTQRLVDENYDLAGPLNGGNL
                     NFESAKEGNLGAEFFSIWVEPTLYKGQYAKRTLELIDAVYQQAAKHPDKMRMAFTADN
                     IVAAHREHKLAALMGIEGGHSIEDSLALLRDYYRLGVRYMTLTWSNSNDWADSSGDIN
                     DPKVKHTQDGLTDFGKDVVYEMNRLGMMVDISHVADKTFYRAVITSRAPVIASHSSAR
                     ALCNAPRNMTDDMLRAVARSGGPGSKGGVVMVNYYAAFISQKYRDAMLAMEPEIAKAK
                     DALKEQYAKAGKQVTYGDLDKLEQQYLDRIPRPPLSDLIDHIDHIAKVAGVDHVGLGS
                     DFDGVSNQLPEGIDSAADLPKITQALMARGYSAEDCDKILGGNLLRVMREVETTAKRL
                     QAESATRPRITEQQPFKK"
     gene            5612..6058
                     /locus_tag="VFA02_10270"
     CDS             5612..6058
                     /locus_tag="VFA02_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10275.1"
                     /translation="MKEQKSAWAALPSFREVREAIEAESPNQTHKPNFLKCLVLYTLV
                     SGAYVFHALSAVNRSGRRIARQLAAFLPGAYLHLVFKDKSDCYGYLGPLSATSFAFKE
                     ATTNSISQIPYNDIESVAGSVAPSAKMPSLLSWLLLMTATAVAARS"
     gene            6107..6808
                     /locus_tag="VFA02_10275"
     CDS             6107..6808
                     /locus_tag="VFA02_10275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU10276.1"
                     /translation="MNAITRFASLAAISSVMVCSGFAQQSTSSSSQELPDSPETQAHI
                     SPQPTGPTAIFDTSMGRITCKLFDKQAPKTVANFIGLAEGTKDWTDPATHQKMHNKRF
                     YDGTTFHRVIPEFMIQGGDPTGTGTGDPGYMFEDEFDPDLNFDVPGRLAMANSGPNTN
                     GSQFFITEVPTEHLNQKHTIFGQCDDSSILVVKSIARVPRDDNDKPIEPVILKKVTIV
                     PAGQPVPPSDSTPKQ"
     gene            6875..7405
                     /locus_tag="VFA02_10280"
     CDS             6875..7405
                     /locus_tag="VFA02_10280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU10277.1"
                     /translation="MARTTGTYAIFNTSEGTIVCRLFEKDAPKTVSNFIDLAEGTREW
                     THPVTNKKSKDKLYDGTIFHRVIPDFMIQGGDPAGSGFGGPGYRFEDETKGSPHNFNQ
                     PGKLAMANAGPNTNGSQFFITVAPTQWLTGKHTIFGEVVEGQDVANKISRAPRGAQDK
                     PLTPVVIESLIIERIA"
     gene            complement(7472..8074)
                     /locus_tag="VFA02_10285"
     CDS             complement(7472..8074)
                     /locus_tag="VFA02_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZU10278.1"
                     /translation="MSLGKAMIHARKQKHAVVPREEVRVHTSAVMQSPVLVLNASYEP
                     INICGARRALVLVLKGVARTEEEQGATLHAARVRMPLPSVIRLLEYRRIPHQTRALSR
                     KNILLRDRNTCQYCGVVLPASELTLDHVVPRSRGGASTWENLVACCHACNRKKGNQLL
                     HELEDMKLLREPRPFTLHTSRHIMRMIGHSDVKWRKYLYY"
     gene            complement(8122..9009)
                     /locus_tag="VFA02_10290"
     CDS             complement(8122..9009)
                     /locus_tag="VFA02_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZU10279.1"
                     /translation="MQSGTSAWRAVARVLRSPADSLCCALFPASCRLCGDSLTRLTRV
                     PVCNSCWNKLPEQYGNLCAICGEALGAQGFGGPQATPGDALCRPCRMAAPPFAWAVAH
                     GLYQGRLRELIHLLKYHGMEPIANRLGSLLATRILRFEGIPQKMLVVPVPLFRTKRRQ
                     RRFNQAELLARATLKALKQLRPEMQLALASGVLERRRATESQAGLTPHQRRANVRGAF
                     FVLHPEKIKDADVLLIDDIYTTGATARACALALRRAGAKSVRVATVARAQRHEMVEIP
                     MHEDVVFWDSGFVPAVRGA"
     gene            complement(9117..10694)
                     /locus_tag="VFA02_10295"
     CDS             complement(9117..10694)
                     /locus_tag="VFA02_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10280.1"
                     /translation="MPSVISEVSAAQLARALEDFLAGYPRAAVLEDGRVLFDMQRARY
                     ALSSDHGRCVLQLWSDERNIVRTVTGISARKDTLRIETRRFGQVRPQTLELVADRDRR
                     TPSARETTRKRYLRVLERVLMRAFPDYSIGDLHSAMDLEHSFGPAYARATLVRGPKAW
                     AVIAVNALEMQSTLDGALTLGILWLNYCREHSGGRRVFEGLKVILPAGKAEVTRARMA
                     WLNPALAKWELYALDERSEELEQLIIEEGNLETRLVHAITPSRVKERMQDAIERVLAL
                     LPDSMHSRVEIYVTSTAEISFRLHGLEFARARHGLASGSFSRQDAITFGAGANETPLT
                     GETEDLFKDLMHRLGEARAATGNARNPLYRMHPERWLESELRKSLAEVEPSVREDCVY
                     SQVPAFSAGDRGMIDLLSVTRAGRLVVLEIKADEDLHLPLQGLDYWIRVRRVHADGDL
                     DQHGYFPGVELSDRPPLLMLVAPALRIHPANETILRHLKPEVEWTLIALDEHWRQQLR
                     VIFRKRREAADSGKPLS"
     gene            complement(10743..11270)
                     /locus_tag="VFA02_10300"
     CDS             complement(10743..11270)
                     /locus_tag="VFA02_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HZU10281.1"
                     /translation="MSEDMRYPIGNFAGPLEQTPEQRRGSIAVLAALPENLRSAVSGL
                     SEAQLDTPYREGGWTVRQLVHHVADSHINAYVRTRKALTEDWPAVAAYDEKIWAELQD
                     AKTLPVEVSLKLLDALHTRWAALFRSLPETEWQRGYVHSENGRQKLADVIQLYDWHCR
                     HHVAHITELRKRNGW"
     gene            11683..12936
                     /locus_tag="VFA02_10305"
     CDS             11683..12936
                     /locus_tag="VFA02_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HZU10282.1"
                     /translation="MLANSIAFDPLNPKAVLSEIPARAGVFALFAADERAEPYVSRTP
                     NLHRRLSRFLDARPTQSKRLRLTEKIARIEWSLTGSDFESLLLLYRLSAQLFGDAVHK
                     RMHLRAPFFLRMAMENAWPRVYVTNRITKSAQEQLFGPFPSRATAEKFADEMLNFFLL
                     RRCHEDLHPDPAFPGCIYSEMKMCLAPCFKGCTDERYAEEAANVYAFLKTRGASLIQK
                     LAAERDTSSAGLDFEKAAQLHAKLQKVESVASLVSPAVQQLSKMRALVLQPSAVSESV
                     AIFLLSNGVITGPAYYAVSGMRLHNEHSGSTSLYVAPVAVEAVPLATSVQLATHDVLQ
                     ERLEDVLARLELQNKKLKAPSQTLSDYLCLFKRWYYRPQTQRTGEIFFADEKGEFQKK
                     QVLRGISRVFSSTQKEEAGDTLRPA"
     gene            complement(12951..15551)
                     /locus_tag="VFA02_10310"
     CDS             complement(12951..15551)
                     /locus_tag="VFA02_10310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZU10283.1"
                     /translation="MPRCIRPFFAVVCIALGPLFTQTAHSQRLPQTVHPEHYSLILTP
                     DLKNATFTGKEKIDVVISEPVDAITLNAAEIKFQSVKTTLDGKTLNAAVSEDAAKEQA
                     TFDFHQKLPAGHLTLNIEYSGILNNELRGFYLSKTAKRNYAVTQFEPTDARRAFPSFD
                     EPAFKAKYDVALIVDKGDTAISNTNIVSDTPGPGEDKHTIRFATTPKMSTYLVAFLVG
                     DFQCTSGESDGVPIRGCATPDKVELTKYAVKAAEFVLHYYDHYFGIKYPMPKLDMIAL
                     PDFEAGAMENFGAITYRETDLLLDEKNASVGAKKNVALVVAHEMAHQWFGDMVTMQWW
                     DNLWLNEGFASWMENKPVAAWHPEWNIPQDVAQQLNGTLNLDAQRTTRTIRAKADTPD
                     EINQMFDGITYGKAGAVLLMVENYLGEQTFRQGVHNYLAAHMYGNATAEDFWNAQTTT
                     SHKPVDKIMESFVVQPGEPILTFDDPKDSSVSVQQQRFFLNPSVKAEQGQSWTVPVCM
                     KTSGKASDCQVISAAQQALNVPQAQFFFANAGGKGYYRSTYPADVYGKIISNVESGLT
                     PEERISLLGDVWAQVRADKMGVGDYLNLAAAVKDDTSASVIQTALSAVGTIDDQIAST
                     DEEHKALAAWVLKNFKPAYGRLGKASDSDSPEKKELRAALFGALGTIGEDPGVISEAK
                     EIAQKYLSDPSSVEPTLAQTAIGVAALHGDAAFFDQLQKTYESSTNPQVQEGALRMLA
                     LFKDPALERRALDYTVSNKVRNQDAAIEFLIMLRGQETRDEAWKYIQQNWDKVKAQLT
                     TSMGAYLVGATGSFCSTQARDQVVGFFSTHKVPASDRSLARAKDQINDCIELRSEQGP
                     KLKEWIEAQK"
     gene            complement(15645..17243)
                     /locus_tag="VFA02_10315"
     CDS             complement(15645..17243)
                     /locus_tag="VFA02_10315"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZU10284.1"
                     /translation="MIWLLRDFDLLSVLLRAATLALEALTLGGVVFLLFIALPGGVDG
                     EHLRKLQRAVAWSAFALGWAEILSIVTSSAILIGGSGFSFHDVLSADFFLLGAALTIS
                     AAALFFLLRLRSGRGLFVCALPAVLVLAASVGMSHAAARLEYRGPLLVLTALHHIGTA
                     AWIGAMPFLLMALRREGRQSAAARYARRFSAMAIVGVAVLLTGGIGMALFYVGSWQGL
                     YGTSYGVMLMAKVYLLLVVLGLGTGNFFLVRQIERSPAPLLVRLRRFSEAEIGLGFTA
                     ILTAASMTSQPPAVDLTRDRLTKHEIVERMRWEWPRLNSPPLKALAPPTPLSVAVPES
                     TFNGGSQSDANDRAWSEYNHHWAGLIVLCAGLFALLTRWRFPGARHWPLLFLGLAVFI
                     LLRADPENWPLGPRPFWASFSGPDVLQHRIYALLIAAFAIFEWAVETGRLKSQWAVYV
                     FPLVCAAGGALLLTHQHALGNIREEMLAEMSHTPIALLGATAGWGRWLELRLPDQRQS
                     KVASYIWPICLVLVGVVLLDYRES"
BASE COUNT         3878 a         5063 c         4772 g         3705 t
ORIGIN      
        1 ggcatacgtc cgtggacgct gttgaagctg tgtgctcgcg acaggaaata ggccgggctt
       61 tttgacgagc agccgatgcc ggagagcttc atcacgcgct cgggctgcac gcccatctcg
      121 tacatcgcat caataatgcg ctcggagata gcgttgtgac cgcatccggc gcagagcgtg
      181 gtcttgccac cacggtattc aagaatcggc aggccgaggc ggttcacctt tggtgctggg
      241 gttggagttg ctgttgttgc catgattaga ttccctcctg cagcacgaat tcgtctgtaa
      301 tcgagcgcgc gtcaatcggc aggccgttga aatgccggat ggagcgcagt ttcaccgcgt
      361 cctcagcagg cagatcgagc ttcagaaggc tgagcatctg cgcgtcgcgg ttctgttcaa
      421 cgatgtacac acggtcgtgc gaagcaacaa actcgtggat gtctttggtg aaggggaagg
      481 cgcgaatgcg gacgtaatcc gtatcaacac cgtattcctt gcggatctgg tcgcggcttt
      541 cctgtacggc aaaatcagag gtaccgtagg caatgatgcc aaccttcgac ttccctgttt
      601 gcaaaaccac cggcgcaggt acctgcgtgc gtgccgtctc aaacttgcga tgcaggcgtt
      661 ccatcagatt ctgatagtca tccgggcgct cggtatagag ggccttttcg ttgtgtccgc
      721 tgccgcgtgt aaagtaggcg gccttcggat ggtccgttcc agggagcgta cggtagggga
      781 tgccatcgcc gtccacgtct ttgtagcgag caaattcgcc cagccgcttc aggtcttcgg
      841 cagtgaggac cttaccacga tccaaaggct tttccggata ctcaaatggc tcggacatcc
      901 agttgttcat gccgaggtcg aggtcgctga ccacaaagac cggggtctgg aagcgctcgg
      961 ccaggtcgaa agcggcaatg gaaaattcga aacactcttt cacattgcca ggcagcaaca
     1021 taatgtgctt tgtgtcgccg tgtgaaagat aggcgataga gaggatgtca ccctgcgcgg
     1081 tgcgcgtggg caggcctgtg gaggggccaa cacgctgaat gtcgaagatg acgccaggca
     1141 gctcggcgaa atagccgagt ccggcaaatt ccgccataag cgaaatgccc gggccagagg
     1201 ttgccgtcat ggagcgggca ccagcccatc ctgcgcccag caccatgcct actgcggcca
     1261 gctcgtcttc cgcctggacg acggcgaacg aggccttgcc atccggctcg acacggtact
     1321 tcttcaggta atcaatcaac tgctctacga cagaggatga tggcgtaatt ggataccacg
     1381 tcactacagt gacgccagcg aacattgcgc ccagagcaga ggcggcattg ccatcaataa
     1441 tgatcttgcc gtctgtctcg tgcatgcgtt cgagcacaaa cgggtcctgc ttggtcagcg
     1501 actgagatgc aaaatcgtat ccggcctgta cggcggccca attcaggtcg gcggccttct
     1561 gctttttggc gaactgacgg cgcagcgcgg cttcgactgc taccatgtcc agcgagagca
     1621 gttgcgccac tacgccgaca tagatcatgt tcttgaccag cttgcgcagg cgggcttccg
     1681 gacagacagc ggcagtcagc ttgtcaaagg gaacgggata acaggtgaca tcgtcgcggt
     1741 attgtttgag attcaatgcc tcttcataga tggcaaccgc tccggcaggc agcgaaagaa
     1801 tgtcttcctt tgccgtctcc ggattcatcg ccaccagaat gtcaatttcc ttcttgcggg
     1861 cgatatagcc gtatttgctg gcgcgaatgg tgtaccaggt gggcagtccg gcgatattgg
     1921 acggaaacag gttcttgccg ctcactggaa tgcccatggc aaaaatgctt ctgagcaata
     1981 cattgttggc agactgcgaa ccggaaccgt tgactgttgc tacctggatg ctgaaatcgt
     2041 tgacgatacg cttggaagcg tcacgtgcga gtgcgcctgc ggcctgctgg cctagcgcaa
     2101 catctcctgt cgccattggg cgatgttccc ttcagtgtga aagtggaaag atacaaatct
     2161 ttcacatttc attatagacg gcataagcag gaattgaggc cctggcatcc ctgaatgggg
     2221 gattttgcgt gtgagcggcg aagaaaaagc actgaatggc tgaccgcgtt ctgcctggaa
     2281 tgatctggcg gatgacccag agaaaagcaa tcacttatgc atcaggcctg ggaccagcat
     2341 tctcttgtat tcggacgcaa agcttcatag aactacgtct gcttcagcaa ggtatacctt
     2401 ctttaattcc tcacatcttc cattgctttc gtcacaacag attgctcctc cgccttcaga
     2461 ggaagcaacg gcacccgggg cactccacca tagtaaccat tcagatcaca cgcaaatttg
     2521 attccgggaa caccgaactt ccccgccaca atgcgtgaag cctctttgat acgctcctgc
     2581 ttcagttggg ccagtttggg gtcgttgtct ttccacgcca tgtagatttc ctggcaggcc
     2641 tgaggagcgc acgcgccgaa ggcgaggact gcgccgctgg ccccggtgtc gagagcttcc
     2701 tttacgttgt caggagagcc acttaatacc tgaaagccga cttccctggt gcgtgttgcg
     2761 atttttggtc ctggaggcgc aatggccgcg ggcgctccca gtcctaaaac atcggcgggc
     2821 acaaatgtgg tgtcagccag tgcctgctcc tgcaacatgc ggttggtgaa tgcagtaaag
     2881 acattggtga ctacggttgt gcgcctggga gcattgcgtg tcacggacac aagctgcgcg
     2941 acgcgttcga tgctgccgct agaatccttg ataccgataa tgttcgggtg ctgcgacaat
     3001 tcggcgacca gttcgatgga aagctcatac tttgtgaatt ttggaatgct gtagagcacg
     3061 accgggagag gcgaacggtc ggctaccatg cgaaagtagg tcagcatctc caacacctgc
     3121 atctgcgggc tgtaatagct cggcgtacgc accagcacag catcatactg ctgcccggcg
     3181 gcaaactcgg ccagcttcag tgttgccacg gcgctttcgc ggcccactcc agcaagcagg
     3241 actttttgtg gtgatgcggc ggcacgcgcc gtccgcagga cttcgcgaga ttcttcatcg
     3301 ctgagcatga cggcctcccc ggttgagccg aggactacca gaccagagac gggcgcgcgc
     3361 gaatagcgct cgacattcat ttccaatttg cggagataaa gcccgccatc agtatggaaa
     3421 ggcgtagtga ttgcgggaaa aatgccttct agcagcatga aactattcta cgtagtctct
     3481 cccgggaatg gcgcggtctg cgcattgaga atacgcagaa tgcgggcctt gatcgagtcg
     3541 taggccggcc tgccggaaaa catgtcgtag tctacgcttt tcccgttacc ctcacctgtg
     3601 atctgaaact gacgctctcc atcagcggcc tgctgcgcgt gtgcctgttc ccaggatttt
     3661 tcatcgaagt cgttgctctc aacattgaat gaccagacaa gtttgccctg ggtattcctg
     3721 acatggaacc ggtaatacac attgaatttg ccgactggct tgaaaaattc gatggcttca
     3781 atgcgatact gctttaccgg aaacaggtcc agcaaaaacc cattggtctg catggcctca
     3841 ggagctttag ggtctgcgtg cagcttgcgc aacaattcgc gctccgcatc ccgttcctgg
     3901 atcttgccgg ccatggcata cgcaaccacg agtgtcgtat gcaccttcca gttgtccgga
     3961 gcaagttgct ttgccttttc cgcatactgg accgccagat tgccctgata aagctccagc
     4021 gccgcgcctg cggcatataa attggccgcc acatcgttgg gattggcctg cagggcctgc
     4081 tggaaggccg cgagtgcctg ctgcggttgg tgacttttca aagcagcgag gccctcgtct
     4141 atgggagaag attggctgta agccaataga gcggcgattc cagttacggc caggcagagg
     4201 ttacgcagta gtctcaccaa gccaacttag cacagaaata gctggtcatt aatgaggagc
     4261 tgtctgctac aatcccgcca acactcgaaa accatgaaaa ccggactcct cgctgctctc
     4321 ttggtcgcat ctgttcttcc tttggccgcc aaaacctctt cgcaaaagac tgtgaaaaag
     4381 cacactgttg atccgctcgc cgtccatcgc aacgctattg tgattgatac tcatgccgac
     4441 accacgcagc gactggtgga tgaaaattac gacctcgctg gtccgctgaa tggcggcaat
     4501 ctcaactttg aatcggcaaa agagggcaat ctgggcgcgg agttcttctc catctgggtg
     4561 gaacctactc tgtacaaagg gcaatatgca aaacgaactc tggaactgat tgacgccgtc
     4621 taccagcagg ccgcgaagca tccagacaag atgcgcatgg cctttaccgc agataacatt
     4681 gtcgccgcgc accgcgaaca caaactggct gcgctgatgg gtatcgaagg cgggcattca
     4741 attgaagatt cgctggcgct gctgcgcgat tattatcgcc tcggcgtcag gtatatgaca
     4801 ttgacctggt cgaattccaa cgactgggcc gactcttctg gcgacatcaa tgacccaaag
     4861 gtaaaacata cgcaggacgg cctgactgat ttcggcaaag atgttgtgta tgagatgaac
     4921 cgcctgggca tgatggtaga catctcgcat gttgcggaca agaccttcta ccgcgctgtg
     4981 attacatcac gcgctccggt catcgcgtcc cactcttcag cgcgtgcttt atgcaacgca
     5041 ccccgcaaca tgaccgacga tatgcttcgg gccgtggcac gcagcggagg cccgggcagc
     5101 aagggcggcg tcgtcatggt gaattattat gcggcattca tcagccagaa atatcgcgac
     5161 gctatgctgg ctatggagcc tgaaatcgcc aaagccaaag acgcgctgaa agaacagtac
     5221 gcaaaagcag gcaagcaggt cacttacggc gaccttgaca agctggaaca gcagtacctt
     5281 gatcgcattc cccgcccccc gctgagcgat ctgattgacc acattgacca cattgccaag
     5341 gttgcaggtg ttgaccatgt tgggcttggt tctgactttg acggcgtctc caatcagctt
     5401 ccggagggaa tcgactccgc cgcagacctg ccaaagatta cacaggcgct gatggcccgc
     5461 ggctattcgg ctgaggactg cgacaagatt ctcggcggca atcttttgcg agtgatgcgc
     5521 gaggtagaga ccacggcaaa gcggttgcag gctgaatcgg ccacgaggcc caggattacg
     5581 gaacaacagc catttaagaa atgaggactg cttgaaagaa caaaagagcg catgggcggc
     5641 gcttccctcg tttcgcgaag tccgcgaggc gatagaagca gagtcgccga atcagacgca
     5701 caaaccaaat tttctgaaat gcctggttct ttataccctt gtctcgggag catacgtttt
     5761 ccatgctctt tctgccgtaa accggagcgg gcgaagaatc gcgcgtcagc ttgcggcctt
     5821 tctcccgggc gcctatctgc atttggtctt taaagacaag tctgattgct atggttatct
     5881 tggccctttg tctgccacct cgtttgcttt taaagaagcg acgacaaaca gcatctccca
     5941 gattccttac aacgatatcg agtcggtcgc ggggtccgtc gccccctcag cgaaaatgcc
     6001 ttctcttttg tcgtggctcc ttctgatgac ggcaacagcg gtcgcggcaa gaagttaggt
     6061 tttcagcgcg taccgccatg ctgtaaccta ccctttggaa atcagcatga acgccatcac
     6121 acgttttgcc tcgctcgcag ccatctcatc ggtcatggtc tgttccggat tcgcgcagca
     6181 atctacttcg tcatcgtcac aggaactgcc ggactcgccg gaaactcaag cccacatcag
     6241 cccgcaaccc accgggccaa ctgcgatctt cgacaccagc atgggccgca tcacgtgcaa
     6301 actcttcgac aaacaggcgc caaagacagt cgctaacttc atcggccttg ctgaaggcac
     6361 aaaggactgg acggacccgg caacccacca gaagatgcac aacaagcgct tctacgatgg
     6421 gaccaccttt catcgcgtga tccctgaatt tatgatccag ggcggcgacc ccaccgggac
     6481 tgggaccggc gatccaggct atatgtttga agatgagttc gatccggacc tgaacttcga
     6541 tgtgcccggg cggctggcga tggccaactc agggccgaac accaacggct cacagttttt
     6601 catcaccgaa gtcccgactg agcatctgaa ccagaagcac actatctttg gacagtgcga
     6661 tgattccagc attctcgtgg tcaagtccat tgcacgcgtg ccgcgcgatg acaacgacaa
     6721 gccaattgag ccagtgattc tgaaaaaagt cacgattgtg cctgcggggc agcccgttcc
     6781 gccatcagat tccacgccga agcaataatg atgactcccg cccttcgctt tacgaaggat
     6841 ggggccccca tgaatttcaa aaaaaggagc taacatggcc cgcaccaccg gaacatacgc
     6901 gatcttcaat accagcgaag gcaccatcgt ctgccgtctt tttgaaaaag acgcacccaa
     6961 aaccgtctcc aacttcattg accttgccga aggcacgcgt gaatggacac atcctgtcac
     7021 caacaagaag agcaaagaca agctctacga tgggaccatt ttccatcgcg tgattcctga
     7081 cttcatgatc cagggcggcg atcctgctgg ctctggcttc ggcggtccag gttaccgttt
     7141 tgaggacgaa accaaaggct ctccacataa ctttaaccag ccaggcaagc tggccatggc
     7201 caatgcaggc ccgaacacca atggctcgca gttctttatc actgttgcac cgacacagtg
     7261 gctcacaggc aagcacacga tcttcggcga agtagtggaa ggccaggatg tggcaaacaa
     7321 gatttcgcgt gcgccgcgtg gcgcgcagga caagccgctt acccctgtag tgattgaatc
     7381 actgatcatt gagaggatcg cgtaacaggt cagggaaaat cagctgtttc gactcagcta
     7441 cgctccgctc aggatgacaa atcctgacgg cttagtagta gagatatttc ctccacttga
     7501 cgtcgctgtg cccgatcatg cgcatgatgt ggcggctggt gtgcagtgtg aacgggcgcg
     7561 gctcccgcag cagcttcatg tcttcaagtt cgtgcaggag ttggttgccc ttcttgcggt
     7621 tgcaggcatg gcagcaggcc acgaggtttt cccaggtgga ggcaccaccg cgcgaacggg
     7681 ggaccacgtg gtcgagcgtc agctctgagg caggcagaac tactccgcaa tactggcagg
     7741 tgttccggtc gcggagcagg atgttcttcc gcgacaatgc gcgcgtttgg tgcggaatgc
     7801 ggcggtattc aagcaggcgg atgaccgagg gcagcggcat acgcacacgg gcagcgtgaa
     7861 gcgtggcccc ctgctcttcc tcagtcctgg caacgccctt cagcaccagc accagcgcac
     7921 gacgcgctcc gcagatgttg attggctcat aggaagcgtt cagcaccagc accggagact
     7981 gcatgacagc gcttgtgtgg acgcgcacct cttcacgtgg aacgacggcg tgcttctgtt
     8041 tacgcgcgtg aatcatggcc tttcccagag acattcctct ccctcacaat ccagagatga
     8101 aatgagcacg gccctgagcg cttatgcgcc gcggaccgca ggaacaaaac ccgaatccca
     8161 gaacaccacg tcttcgtgca tggggatttc aaccatctca tgccgttgcg ccctggccac
     8221 tgttgccacg cgcacactct ttgctccggc gcggcgcaag gccagcgcgc atgctcgtgc
     8281 tgtagcgcct gtggtgtaaa tgtcgtctat cagaagcacg tccgcgtcct ttatcttttc
     8341 cggatgcagg acaaaaaaag cgccgcgcac gttggcgcga cgctggtgcg gggtcagccc
     8401 cgcctgactt tctgtggcac gccgcctctc cagaacaccg gatgccagcg caagttgcat
     8461 ttcaggacgc aactgcttca aggccttcag tgtcgcgcgc gccagcagtt cggcctgatt
     8521 gaaccggcgc tgtctgcgct tcgttcggaa gagcggcacg ggaacgacca gcatcttttg
     8581 cggaatgccc tcaaagcgca gaatccgcgt tgcaagcaga ctgcccagcc tgttggcgat
     8641 cggttccatt ccgtggtact tcagcaggtg aatcaactcc cgcaaccgcc cctgatacag
     8701 cccatgcgca actgcccagg caaaaggagg cgctgccatg cggcagggac ggcacaaagc
     8761 atcacctgga gtggcttgtg gtccgccaaa tccctgcgcg ccgagggcct cgccgcagat
     8821 ggcgcaaaga tttccatatt gctcagggag tttgttccag caggagttgc aaacaggaac
     8881 gcgggtgagc cgcgtaagag agtcgccgca aagccggcaa gaggcgggaa ataaagcgca
     8941 gcacaggctg tctgccggac ttcgcaacac gcgagcaacc gccctccatg ccgaggtacc
     9001 ggactgcatc acatccagcg tttgtgctac acgctgactg ctgaagacac acctctcaca
     9061 gcaggttttc cgctgttggc aaaagtatac accgaaaact tgcacaattc caggtgctat
     9121 gagaggggct ttccggaatc tgcggcttcc cttctcttgc ggaaaatgac tcgcagctgc
     9181 tgcctccagt gctcatcgag agcaatcagt gtccattcaa cctctggttt caggtggcgc
     9241 agtatcgtct cattcgccgg atggatgcgc agcgccggag cgacgagcat cagcagcggc
     9301 ggacggtctg acaactctac tccggggaaa tagccgtgct ggtccaggtc gccgtcggca
     9361 tggacgcgcc ggacacggat ccaataatcc aggccctgca agggcagatg caggtcttca
     9421 tccgccttga tttcaagcac caccagccgt cctgcccgcg tcacgctcag caggtcaatc
     9481 atgccgcggt cgccggcaga aaacgccggc acctgtgagt agacgcaatc ttcccggacg
     9541 gatggttcca cttcggcaag gcttttcctc agctcagatt ccagccagcg ctccggatgc
     9601 atccggtaaa gaggattgcg agcgttgcct gtggccgcgc gcgcttctcc caggcgatgc
     9661 atgaggtctt tgaacaggtc ttcggtttcg ccggtcagcg gagtttcatt tgcgcccgcg
     9721 ccgaaggtaa tggcgtcctg tcgcgaaaaa gaaccggaag ccaatccatg acgcgcacgc
     9781 gcgaactcca gcccatgcag gcgaaagctg atttcggccg tgctggtgac atatatttca
     9841 acgcgcgaat gcatggaatc cggcagcagt gcgagtacac gctcaattgc gtcctgcatt
     9901 cgctctttca cccgtgacgg agtaattgcg tggactaggc gcgtttccag attaccttct
     9961 tcgatgatga gttgttccag ttcttctgag cgctcgtcca gggcgtacag ctcccatttg
    10021 gcgagcgcgg ggttgagcca cgccatgcgc gcgcgtgtta cttctgcctt gcctgcgggc
    10081 agaatgacct tcaggccttc aaagactctc ctcccgccgc tgtgttcacg acaataattg
    10141 agccacaaaa tgccgagggt gagagcgcca tccaatgtgg actgcatctc cagcgcgttc
    10201 actgcaatca cggcccaggc ctttggccca cgcacaagtg ttgcgcgagc ataagcaggc
    10261 ccgaaactgt gctccaggtc catggcactg tgcaggtcac caatgctgta gtcaggaaag
    10321 gcccgcatca gcacgcgctc cagcacgcga agatagcgct tgcgtgtggt ttcgcgcgcg
    10381 gagggagtgc ggcggtcgcg gtcggccacc agctccaggg tctgtggcct gacctggcca
    10441 aagcggcgcg tctcaatgcg cagcgtgtct ttgcgcgcgc ttattccggt gacggtacgt
    10501 acgatattgc gctcatctga ccagagctgc aaaacacatc gtccgtggtc ggaagaaaga
    10561 gcataccgcg cccgctgcat atcgaaaagc acacggccat cttccagcac agcggcacgc
    10621 ggatacccag ccagaaaatc ctcgagcgcg cgggccagct gcgcggctga aacttcactg
    10681 ataacagatg gcattaagaa aactatgctt gcccataaga agcgcgagca agcattttca
    10741 tgctaccagc cgttgcgctt ccgcaattcg gtaatgtgtg ctacatgatg acggcagtgc
    10801 cagtcataaa gctggatgac atcggccagc ttctgacgtc cgttttcaga atggacatat
    10861 ccacgttgcc actcggtctc cgggagagac ctaaacaagg cggcccagcg cgtatgcaga
    10921 gcatcgagca gcttcagaga gacttctacc ggaagcgtct ttgcgtcttg cagctcggcc
    10981 cagattttct catcgtatgc cgcaaccgct ggccagtctt cggtgagcgc cttgcgcgtg
    11041 cgcacataag cattgatgtg gctgtcagcc acatgatgca ccagctggcg caccgtccat
    11101 ccgccctcac gatagggcgt atccaattgc gcctcagaaa gaccgcttac cgcggagcgc
    11161 aggttttccg gcagtgctgc caggaccgca atggaaccac ggcgctgctc tggcgtctgc
    11221 tccagtggac cggcaaaatt cccgatggga taacgcatgt cttcgctcat acgacgaaca
    11281 gtatcacaga agacgcgcgt ccggcactat tgcgcctgat ggtgataatg gtgcgcccgg
    11341 tgatgacggt gacggtgcgt tctcgcaaat gctgaggatg aaaccagcaa aaccaaagac
    11401 agaatgatga gcttcttcat gccgcgatgg tagcaaaaaa agaaagcaaa ccagcttgtg
    11461 gaaatcatgg gatggaacca aacgcgccaa ctctgctcag gcagactgtt tccggtttgc
    11521 ggagctttgt agacggcgcg attgcgaccg gagaataatc aagctatagc atcttcacag
    11581 atggcctgga ccatgccacg aaactacagt cgccgctacc gacagggagt cggcgacagt
    11641 gtaaatggga atgtacagaa tctgtgaaaa tgccatagct ccatgcttgc gaactctatt
    11701 gcatttgatc cgttaaaccc gaaggctgtg ctttcagaaa ttcctgcgcg ggccggggtc
    11761 ttcgcccttt ttgccgccga tgaacgcgcc gagccatacg tcagccgcac ccccaacctg
    11821 catcgcagac tcagccgctt tcttgatgcg cgcccgacgc aatcgaagcg cctgcggctg
    11881 acagaaaaaa ttgcgcggat tgagtggtcg cttaccggat cggatttcga gtcgctgctg
    11941 ctgctctatc gtctatcggc gcagctattt ggcgatgccg ttcacaagcg catgcacctg
    12001 cgcgcgcctt tttttctgcg catggcaatg gagaatgcct ggccgcgcgt ttatgtaacc
    12061 aatagaatta caaaatcggc acaagagcag cttttcggcc catttccctc ccgcgcaacc
    12121 gcggagaaat tcgccgatga gatgctgaat ttcttcctgc tgcgccgctg ccatgaagac
    12181 ctgcatcctg atccggcctt tcccggctgt atttattccg agatgaagat gtgccttgct
    12241 ccctgcttca aaggctgcac ggatgaacgt tatgccgagg aagccgccaa cgtttatgct
    12301 tttctgaaga ctcgcggcgc cagcctgatt cagaaactgg cagcagagcg cgacacttca
    12361 tcggcagggc ttgattttga aaaagccgcc cagctccatg caaagctgca aaaagttgag
    12421 tccgttgcat cgctggtttc tccggctgtg cagcagctat caaagatgcg cgcactggtc
    12481 ctgcagccct ctgctgtgtc ggaaagcgta gcgatctttc tgctctcaaa tggagtaatt
    12541 accggccctg cttattacgc tgtctcagga atgaggctgc ataatgagca ctccggctct
    12601 acatctcttt atgtcgctcc cgtcgcggtg gaagcagtgc ctctggcaac atctgtgcaa
    12661 ttggccacgc atgatgtgct gcaggaacgg ctggaagatg tgcttgcgcg gcttgaattg
    12721 caaaacaaaa agctgaaagc tcccagtcag accctttccg attatctctg cctgttcaaa
    12781 cgatggtatt accgcccgca aacacagcgt actggcgaaa ttttcttcgc agatgagaaa
    12841 ggtgagttcc agaaaaaaca ggttttgcgt ggaatttcgc gcgtcttttc cagcacgcaa
    12901 aaagaagagg ccggagacac gctccggccc gcttaagaca tcgggcgccc ctatttttgg
    12961 gcttcgatcc actcttttag cttcggccct tgctcactgc gcagttcaat gcagtcgttg
    13021 atctggtctt tggcccgcgc cagcgaccgg tcagacgcag gaactttgtg cgtggagaag
    13081 aagcccacta cctggtcacg ggcctgcgtg ctgcagaagc ttccggtggc gcccaccaga
    13141 taggccccca tggaagttgt aagttgcgcc ttcaccttgt cccagttttg ctggatatat
    13201 ttccatgctt catcgcgtgt ttcctgccca cgcagcatga tcaggaactc aatggccgcg
    13261 tcctggttgc gcactttgtt tgaaacagtg taatcaagcg cacggcgttc gagggccggg
    13321 tccttgaaga gggccagcat acggagcgcc ccttcctgca cctgcggatt ggttgagctt
    13381 tcgtaggtct tctgcaactg atcgaagaag gccgcatcac catgcagagc agccactcca
    13441 atcgccgtct gcgcgagcgt cggctccact gaggaaggat cgctgagata cttctgcgca
    13501 atctctttcg cttcagaaat tacgccgggg tcttccccga tggtccccag cgcgccaaac
    13561 aatgcagcac gcagttcctt cttctccggc gaatcgctgt ccgacgcttt gcccaggcgt
    13621 ccgtaagcag gcttgaagtt cttaagcacc catgcagcca gcgccttgtg ctcttcatcg
    13681 gtcgaagcaa tctggtcatc aatggtcccg actgcgctca acgctgtctg aatcacagaa
    13741 gcgctcgtat cgtcctttac tgctgccgca agattcagat aatcgcccac acccatcttg
    13801 tctgcgcgca cctgcgccca tacatctcca agcaggctga tccgctcttc cggcgtcaat
    13861 ccagattcga cgttcgaaat aatcttcccg tacacatctg ctggataggt gctgcggtaa
    13921 tagccttttc ctcctgcatt ggcaaagaag aactgtgcct gcgggacatt gagcgcttgc
    13981 tgtgctgcag aaatcacctg acaatcactc gccttaccag aggtcttcat gcacaccggt
    14041 accgtccacg actgcccctg ctccgccttc acgctggggt tcaggaagaa ccgctgctgc
    14101 tgcacagaga cgctgctgtc ctttggatca tcgaaggtca ggattggctc cccaggctgg
    14161 acgacgaagc tttccatgat cttgtccacg ggtttatggc tggttgtggt ctgcgcgttc
    14221 cagaagtcct ccgcggtcgc attgccgtac atatgcgccg ccagataatt gtgcacgccc
    14281 tgccggaagg tctgttcacc aaggtaattc tccaccatca gcagcaccgc gccggctttg
    14341 ccgtaggtga tgccgtcaaa catctggttg atctcatccg gcgtgtctgc tttggcgcgg
    14401 atggtccgcg tcgtgcgctg cgcatccagg ttcagcgtgc cattgagctg ttgggccaca
    14461 tcctgcggaa tgttccactc cggatgccat gcggccacag gcttattttc catccacgaa
    14521 gcaaaaccct cgttcagcca caggttgtcc caccactgca tcgtcaccat gtcgccaaac
    14581 cattggtgtg ccatctcgtg cgcaacaaca agggcaacat tcttcttcgc gcctacagag
    14641 gcgttcttct cgtcgagaag caggtcggtt tcgcggtagg taatggctcc gaagttttcc
    14701 atggcgcccg cttcaaagtc cggcagcgcg atcatatcca gcttcggcat cgggtacttg
    14761 atgccgaaat aatggtcata gtagtgcaga acaaactccg cggccttcac tgcgtatttc
    14821 gtcagttcca ctttgtccgg ggtcgcgcat ccgcgaatcg gcactccatc gctttcaccc
    14881 gatgtgcact ggaaatcgcc gacaagaaat gcaaccagat aggtagacat cttcggcgtc
    14941 gtcgcaaacc tgatggtgtg cttgtcctcg ccgggaccgg gggtgtctga cacgatattt
    15001 gtgttggaaa tcgctgtgtc gcccttgtct acgatcagcg ccacatcata cttcgcctta
    15061 aaggccggct cgtcaaagga tgggaaagct cggcgcgcat cggttggctc aaattgtgta
    15121 acagcatagt tacgctttgc ggtcttagag agataaaagc cgcgcagctc attgttcagg
    15181 atgccggaat attcaatgtt gagcgtgaga tggcctgcgg gcagcttctg gtgaaaatca
    15241 aatgtcgcct gctcctttgc ggcatcctct gaaaccgcag cattcagagt ctttccgtca
    15301 agcgttgttt tgacactctg gaacttgatc tcggctgcat tcagcgtgat cgcatccaca
    15361 ggctcggaga tgacaacatc aattttttcc ttacctgtga aggtggcgtt cttcaggtcc
    15421 ggcgtcagta tcagactata gtgttccgga tggactgttt gaggaaggcg ctgcgagtga
    15481 gctgtctggg taaagagagg gccgagggca atacatacaa cggcaaaaaa cgggcgaata
    15541 catcgcggca tagggactcc ttgaaaggct gaaacggtgc ttttgctgga atgcgtcaaa
    15601 gctctgtatt taccatatcg caaacgcaac agaagattgt ccggtcacga ctcgcgatag
    15661 tcaagcaaaa ccacacctac cagcacaaga caaatgggcc agatgtacga cgcaactttt
    15721 gattggcgtt ggtctggaag gcgcaactcc agccagcggc cccatccggc agttgcgcca
    15781 agcagggcaa ttggggtgtg gctcatttcg gcgagcatct cttctcggat atttcccaga
    15841 gcgtgctgat gggtcagcaa aagcgcgcct ccggcagcgc ataccagagg gaaaacatac
    15901 acggcccact gcgatttgag ccggcccgtc tctaccgccc attcaaagat ggcgaaggcc
    15961 gcaatcaata acgcatagat gcggtgctgt aacacgtctg gcccactaaa gctggcccag
    16021 aaaggacgcg gccccagggg ccagttttcc ggatcagcac gcaacagaat aaacaccgcc
    16081 agccccagaa acagcagcgg ccagtggcgc gcgcctggaa accgccatcg tgtcagcaac
    16141 gcaaacagcc cggcgcagag tacaatcagg ccggcccagt ggtggttgta ctcgctccat
    16201 gcgcgatcat tggcgtcgct ctggcttcca ccgttgaacg tggactccgg gaccgccaca
    16261 ctcaagggcg taggtggcgc gagcgctttg agcggcggac tatttagacg cggccactcc
    16321 caccgcatcc gctcaacaat ttcatgcttc gtcaggcggt cccgggtaag atccactgcc
    16381 gggggctgcg atgtcatgga ggccgccgtc agaattgccg taaagccaag tccgatctct
    16441 gcttcgctga atcgccgcag ccgtaccagc aacggcgcag gactgcgctc tatctgccgc
    16501 actagaaaaa aatttcctgt ccccaggccc aggaccacca gcagcagata gactttcgcc
    16561 attaacatga ctccgtagct tgtgccataa agcccttgcc acgaacctac ataaaacaat
    16621 gccatgccga ttccgcccgt cagcagcaca gcaacgccca caatcgccat cgcggaaaac
    16681 cgccgcgcat agcgcgcagc agccgactgc ctgccctcac gccgcagcgc catcaacagg
    16741 aatggcattg cgccgatcca tgcagccgtg ccgatgtggt gcagcgcagt cagaacaagc
    16801 agaggaccgc gatactccag ccgcgcggct gcatgactca ttcccacaga ggccgccagc
    16861 accagcaccg ctggcaaagc acatacaaaa agccctctgc cagaacgcag ccgcagcaga
    16921 aaaaacagcg ccgcagcgct aattgtaaga gcggcgccca gaagaaaaaa atccgccgag
    16981 aggacatcgt ggaagctaaa gccggatccg ccgatcagaa tggcagagct tgtgacgata
    17041 gaaagaattt ccgcccaacc taaagcaaat gcagaccagg ccacagcacg ctgcaacttt
    17101 cgcaggtgct caccgtccac tccaccaggc aaagcgatga acagcagaaa caccacccca
    17161 ccgagtgtca gcgcctcaag tgccagcgtg gcagcccgca acagcacgga gagaaggtcg
    17221 aaatctcgaa gtagccagat caatttgcta ttgaacggta aagggaattt ctccgcgcgt
    17281 aatgtggccg tcggcagcca gcgcctgcca gcggattgtg tattgcccgg cagccagatg
    17341 aatgctctgc gcagaaagag catccggagc agactgggct tcgagcgtga gcgtctgtac
    17401 cttaccatcc ggcccaac
//
LOCUS       DASRBV010000279         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_528805, whole genome shotgun sequence.
ACCESSION   DASRBV010000279 DASRBV010000000
VERSION     DASRBV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528805"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..824
                     /gene="purQ"
                     /locus_tag="VFA02_10320"
     CDS             93..824
                     /gene="purQ"
                     /locus_tag="VFA02_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZU10285.1"
                     /translation="MKFGVLVFPGSNCDYDTYHVVSEISRQPVTFLWHDSPELQNCDA
                     ILVPGGFAYGDYLRTGAIARFSPVMQAVKKFAAEGGPVLGICNGFQILCEAGLLPGAL
                     MRNAGLKYICKQVYLRTETADSPFTQTLRKGEVLKIPIGHMEGNYFCDAETLAKLEAD
                     DRIAFRYSTPRGEITQEANPNGSLSNIAGVLSEGRNVLGMMPHPDRSAEELLGSSDGY
                     KIFASLINYLAAKEKSSVSQELAVR"
     gene            840..1634
                     /locus_tag="VFA02_10325"
     CDS             840..1634
                     /locus_tag="VFA02_10325"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZU10286.1"
                     /translation="MIDIHHHLLFGLDDGSPDIDTSAAMAEAAANDGITHVVCTPHAN
                     DRYRFDPQTNSERLAALRERVDGRITLGLGCDFHLSYDNIEDALKNPAKYTINQKNYL
                     LVEFADLMIPASMSETFFEMGVRGIRPIITHPERNFTIQRHPERMVPWLREGCLVQIT
                     ASSLTGRFGRTAQSFAFDFLDKNWVHFIATDAHNLTSRPPVMSEAYKVIEDKYGRETA
                     QRVCVENPKAAFDGTPLPPQPEPIGLHGEMEAKQKKGLFSRLFATK"
     gene            1806..>2977
                     /locus_tag="VFA02_10330"
     CDS             1806..>2977
                     /locus_tag="VFA02_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10287.1"
                     /translation="MQTLRQDISYALRQMRQAPIFTLTAMLTLALGIGATTAIFSLIH
                     TVMLKSLPVADPSRLYRVGSGDDCCEEGGPQDDWGMFPYSLYLRMKDSSPEFEQMTAF
                     QAGAWQYSVRREKTERQARPIYGEFVSGNYFSTFGIQAFVGRTITPADDQPSAPPVAM
                     LSYRAWQQEYGSDPSVIGASFVVEGHPFTVVGITPPGFFGETLRSNPPDMWFPLEQEP
                     MMKGEDSLVKKPTTAWLRVIGRLRPGASVNGMSARYTAMVRSWLQNEFVHDVGAPQEF
                     QAQINATLPKQFVKVIPAGTGVSVMKADYEDSLRILLIVCCLVLLIACANIANLLLAR
                     GAARRSQTSVRLALGAPRKRLVQQSLTESLVLSLFGGAAGIAVAYLGVKLIVGLAFG"
BASE COUNT          628 a          747 c          918 g          684 t
ORIGIN      
        1 tccggtttcc atgtggtcag aaagtcttag atggctgata tgtgccagag aattcggcgg
       61 cacatcctgc gtgaagctgt tagcctagaa gtatgaaatt cggcgtgctg gtctttcccg
      121 gatcgaactg cgattacgac acctatcatg tagtgtcgga gatttcgcgg cagccggtga
      181 ctttcctctg gcatgactca ccggagcttc aaaactgcga cgccattctc gttcccggcg
      241 gctttgctta tggggactat cttcgcacgg gtgccattgc gcggttttct cctgtaatgc
      301 aggcggtgaa gaagtttgcg gcggagggcg gcccggtgct gggcatctgc aatggcttcc
      361 agattctgtg cgaggcgggg ctgcttcccg gcgcgctgat gcgcaatgcc ggactgaagt
      421 atatctgcaa gcaggtctat ctgaggacag agacggcgga ctcacccttt acccagacat
      481 tgcgcaaggg tgaggtgctg aagattccga tcgggcacat ggaagggaac tatttctgtg
      541 atgctgaaac tctggcgaag ctggaagctg acgaccggat tgcgttccgg tactccacgc
      601 cacggggtga gattacgcaa gaagcgaacc cgaacgggtc gttgagcaat attgccggag
      661 tgttgagcga aggccgcaac gtgctgggca tgatgccgca tcctgaccgg tcggcagagg
      721 agctgctggg ttcgtcggat ggatacaaga tttttgcatc gctgataaat tatcttgcag
      781 cgaaagaaaa gagttccgtt tcccaggaac tggcggtaag gtagcaggtc ccagatttca
      841 tgattgatat tcatcaccac ttgctgttcg gtctggacga cggatctccc gatattgata
      901 cttcagcagc catggcagag gcggcggcca atgacggtat tacgcacgtg gtctgcacgc
      961 cgcacgcgaa tgacagatac cgttttgatc cgcagacaaa tagtgaacgg ctggctgctc
     1021 tgcgtgagcg cgtggatggc aggataacat tggggctggg ctgtgacttc catctttctt
     1081 acgacaacat agaagacgcg ctgaagaacc cggcaaaata tacgatcaat cagaagaatt
     1141 atctgctggt ggaatttgcc gatctgatga ttcctgccag catgagcgag acattttttg
     1201 agatgggggt ccggggcatt cggcccatca tcacacaccc ggagcggaac ttcacgatcc
     1261 agcgccatcc ggaacgcatg gtcccgtggc tgcgcgaagg gtgtcttgta cagattacgg
     1321 cctcatcgct cacgggacgc tttgggcgca cggcacagtc ttttgcattt gattttctgg
     1381 acaagaactg ggtgcacttc attgccacgg acgcacacaa tctcacctcg cggcctccag
     1441 tgatgagcga ggcatataaa gtgattgagg acaaatacgg aagagagacg gcccagcgtg
     1501 tctgtgtaga gaatccaaag gctgcatttg acggcacacc gctgcctccg cagccggagc
     1561 cgatcggact ccacggcgag atggaagcga agcagaagaa gggccttttc agccggttgt
     1621 ttgctacgaa gtagttgaga gatccggttt ttctattctg cgctcatgtc cggaaatgga
     1681 catgcactgt ccggaagtgg acatcccggc catgtagtta tctatatact gacatttcat
     1741 atcaataact taggtttgcg gaactttggc ccttgcagtg ctcgtatatg cgacagaggc
     1801 ccacgatgca aactctccga caggacattt cttatgcgtt gcggcagatg cggcaagccc
     1861 ccatctttac gctgacggcg atgctgacac tcgcgcttgg cattggcgcg acgacggcca
     1921 tcttttcgct gatccacaca gtcatgctga agtctttgcc ggtggcggac ccgtcgcggc
     1981 tgtaccgggt ggggagtggc gatgactgtt gcgaggaggg tgggccacag gacgactggg
     2041 ggatgttccc ttattcgctc tatctccgga tgaaagatag ttctcctgag tttgagcaaa
     2101 tgaccgcctt tcaggccggg gcctggcaat acagcgtcag gcgcgagaag acagagcggc
     2161 aggcgcggcc gatttacgga gagtttgttt ccggaaacta cttttctact tttgggatac
     2221 aagcgtttgt ggggcggacg attactcctg cggacgacca gccctctgcg ccgcccgtgg
     2281 ccatgttgag ctatcgggca tggcagcagg aatacggctc ggatccatct gtaattggcg
     2341 cgtcctttgt tgtggagggg cacccgttta cggtagtcgg gattactcca cctgggttct
     2401 ttggcgaaac actgcgcagc aatccacctg atatgtggtt cccgctcgaa caggagccaa
     2461 tgatgaaggg ggaggactca ctggtaaaga aaccgaccac agcctggctg cgagtgattg
     2521 ggcggctgcg gccgggagcg tcggtgaatg gaatgtcggc gcgctacacg gcgatggtgc
     2581 ggtcatggct gcaaaatgag tttgtgcacg atgtgggtgc tccgcaggaa tttcaggcac
     2641 agatcaatgc aacgctcccc aaacagtttg tgaaggtcat tccggcgggc acaggcgtca
     2701 gtgtgatgaa ggcggactat gaagacagtc tgcgcatcct tctgattgtc tgctgccttg
     2761 tgctgctgat tgcatgcgcc aatattgcga accttctgct ggcgcgcggg gcggcgcggc
     2821 gcagccagac atctgtgcgg ctggccctgg gggcaccgcg gaaaagactt gtgcagcaat
     2881 cgctcacaga aagcctggtg ctttctcttt ttggcggagc ggcgggaatt gcggtcgcct
     2941 atctgggggt aaagctgatt gtgggtctgg cctttgg
//
LOCUS       DASRBV010000280        11397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_322970, whole genome shotgun sequence.
ACCESSION   DASRBV010000280 DASRBV010000000
VERSION     DASRBV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322970"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFA02_10335"
     CDS             complement(<1..1211)
                     /locus_tag="VFA02_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10288.1"
                     /translation="MATQRTATIRSGWLWTGVIITLLLVFYIAHRLTRTTLPVRAATV
                     VRSSLRSTTSTNGVVDPVSRYEATAPFPGIIKAIYVHEGDQVPSGKLLLSMDDTDARA
                     KLATALANLKGAQAAYDATVNGGTQEELLSLNGQIQSAMMDRAQAQNSLATLEKLQMQ
                     GAASPSEVAAAKDRLAAANNSLAVLQKKKTDRYGSTDVAHAKASLEDAQAAYAAALQV
                     VNQANVKAPFPGTVYSIPFKATEFVEQGKTLLEMADLKKMQVLAYFDEPEVGRLKEGM
                     PVQITWDARPDLEWHGHIERVPSTIIRYQETRNVGQAVIAIDDADGQLLPNTNVTVKV
                     TISDSANVLNVPRDALHTEQGKFYVYRIVDGVLRRTQVTTGASNITQIEILSGLNEGD
                     AVALGTTNGQPL"
     gene            1298..1696
                     /gene="queF"
                     /locus_tag="VFA02_10340"
     CDS             1298..1696
                     /gene="queF"
                     /locus_tag="VFA02_10340"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZU10289.1"
                     /translation="MALSERYTDEHAKAGLDYKFPEIETWKNQFPAYEIVIDDPEFTS
                     VCPKTGLPDFGVITLRYMPRELCLELKSYKEYLFCYRNLGIFQENVVNQVLEDVVKAC
                     NPVWAVVKGEFRPRGGISTTVEARWPRSRE"
     gene            1742..2995
                     /locus_tag="VFA02_10345"
     CDS             1742..2995
                     /locus_tag="VFA02_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10290.1"
                     /translation="MKAKAQARPTAAQTAGMALFLLTCLNLFNFIDRYVLPGVQPLIQ
                     KEFLVNDAQIGLLTSAFFFTYMIVAPLTGWLGDRFPRRPLIVTGALIWSAATLLTATV
                     HSYETLLVRHAVVGIGEATFSIFAPALLSDFYPEIDRNRVLSIFYITIPVGAALGYLT
                     GGVLGQHYGWRVPFFVAALPGVLIAAAFWFFVKEPPRGSCDELKPTFDRTTLSGLLRN
                     PAFWTATLGMAMWTFAVGGISTFLPTFFVRFGGYTVATAGIVTGAITAIDGLVGTIAG
                     GWIGHRWVRKNHRALYLVSAWSMMLAIPACAATLFGPRSWLVPGAFVAELLLFLNNGP
                     LNACLVNSVAASIRSSAIAINLFIIHALGDAFSPRLIGRVSDATTSLRTGMGMTLIAL
                     GIASAILFTGSRFAPLLPESEDVSF"
     gene            complement(3159..4010)
                     /locus_tag="VFA02_10350"
     CDS             complement(3159..4010)
                     /locus_tag="VFA02_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10291.1"
                     /translation="MADFSIRRTFRRQPLAVIGAALLVLFVGIALFAPLIAPYNPAAI
                     NLEHRLTPPDHTHWFGTDELGRDIFSRTLYGARLSLFVASSVVACSLFLGLVLGGIAG
                     YYGGVLDTALNTFLMNAFLALPGILLAIAFVAFLGPGLINLILALSIGGWVGYARLVR
                     AQVLAAREREFVEAARALGASDLRIFTKHILPNILQPVIVQAAIGLAGTVLAEATLSF
                     LGLGVPPPAASWGAMLNDARSHLFDAPHLVIFPAIAVMLCVLSFNFLGDALRDYLDPR
                     TRMSVGM"
     gene            4260..7586
                     /locus_tag="VFA02_10355"
     CDS             4260..7586
                     /locus_tag="VFA02_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10292.1"
                     /translation="MKTILRLVLLAVFLCPCTAALAQTAGAGTISGTVTDTGGAVIGN
                     ATVTVTNTDTGAARSITTNNAGSYVAPFLQPGHYSISVSAPNFGNVEQKNITLHVGDT
                     LTVDAALPPAAVQSTVQVSAAAPLLDPERTEVSQVVSEKLVQNLPVNGRRWDDFVLLT
                     PNVVNDGGSGLVSFHGISGLYNSNYVDGANNNQMLFSEARGRASGAPYIYSLDSIKEF
                     QVATATYSAEFGQAAGGQVNAITKSGTNDLHGDLFYYLRYPSLNALDPYNKGQGIANH
                     NPLLLTQPIHQQQQFGGSAGGPIVRDRLFGFFTYDGFRRVNPVLYSSTANISLTPAGS
                     FGDHGNNITPAQCPASIGGPGSANCTNAIKYLQALSGSYPRTGVQDIFFPRLDWQMND
                     RNHVFANFNFSNYHSPSSYASSPSFTNSSVTTNGSTNYHERFVVAQWTDALTDHSVNE
                     VRFQWGRDLETAGANAPAPSVSLGGVQTYGMPNALPRTAEPDEHRYQITDVFSTTHGR
                     HTLKAGGDLNLVHEVMINLFQGGGLYSYTKGVGPSDNFAAWVQDFFPATSSDPLAGTH
                     YDTFVQVIDPITHTGKDDFWMKMIDGFVEDSWKITHDLTFDLGVRYDVQLTPQPLHPN
                     TSTALAAHYNQTLKNVLDRVQPRIGFAWNPIQGTVVRGGYGLFSALNQGSTYYAMRVE
                     NGVFQTNYSFVGAGGAGGKLPVGAPLFPNVLFTPPGPGLNSAPLYGAPSPISYGGAAS
                     TVTPLPSGLKPVPSFHGLDPNFVPPLAHEMQLGVEQQMPGRMTLSIGYVGTRALRLPV
                     FVDGNLIGQKPSGTKTYQQVDKSGKPIGKPFTLPFYLPTDRRDTTIGSLNTGFSSINT
                     WYNSMAVTIKKPFEHGLELLVNYTWAKALDLGQVQGAYGTFYGSDFPLDPNDLRAEYG
                     RSDLDQRNRFIGSLVYQPQLYQSNRWVRHLLDDFIFSGTATETTGFPVTATLSGYPSG
                     GADGGVTGGVMSSSSGTATNGRVPNQVAGRNAFPGPGVRNIDFRVTRNFPITEKINFQ
                     ILAEAFNLLNQRINQSVNTTGYSLLTPGKAGACQSYSGSAITGGCIAPTTGVPLGTPT
                     STSSLLYGARQLQFSTKLFF"
     gene            complement(7668..8330)
                     /locus_tag="VFA02_10360"
     CDS             complement(7668..8330)
                     /locus_tag="VFA02_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU10293.1"
                     /translation="MFKVILADNQAVYRAGSAKILAMEDDFRIIAQCADSDRLYQALD
                     SFRNTVLLFTSALNLDLPLLMERVAKSGCRSIVVLENQESPRHWLEHGVHGILYRSVS
                     GSTLIDCVRRVAMGEQYLQPALEVLRRTQNEDEVGARVRDRLTPKEMRIVALIIHGCK
                     NKEIAMRLGTTEQVVKNYLRSIYDKTGVSDRLELALFTIHHKMLAAAAEAVGNEMMMQ
                     AG"
     gene            8766..10418
                     /locus_tag="VFA02_10365"
     CDS             8766..10418
                     /locus_tag="VFA02_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU10294.1"
                     /translation="MAKPVILAVDDDISVLEAVIQDLRRQYGANYRILRAGSGQAALD
                     TCTQLKQRGDAVALFLSDQRMPGMSGVEFLEKAQAIYPEARRALLTAYADTEAAIKAI
                     NTAKINYYLTKPWDPPEEKLYPVLDDLLENWKEGYKPPFEGLRVVGPRWSARDHEVRD
                     FLSRNQVPYVWLDPERNDEAVQLLEQYQINDQKLPAVLFPDGSSLVQPSRIELAARIG
                     LRTQAEKDFYDVAIIGAGPAGLAAAVYAASEGLKAIVVEPDAPGGQAGSSSRIENYLG
                     FPSGLSGADLARRAYAQASRFGAEFLTQRATGIRTEAPYHFVQLADGREISCHIVLVS
                     TGVDYRRLSIPGIEQFTGAGVYYGAALTEALSCKDEDIYIVGGANSAGQAAVHFARYA
                     RKVTMLVRADSLEKSMSKYLINQIAGTSNIEVQTGAEVQEVAGNGRLECLKVRNRHGE
                     LACQASALFIFIGAEPKTEWLPENVMRDPRGFVLSGPDLLIEGKMPKSWKEDRPPFLL
                     ETSTPGIFVAGDVRHGSVKRVASAVGEGSISVQFMHQYLARF"
     gene            10426..>11397
                     /locus_tag="VFA02_10370"
     CDS             10426..>11397
                     /locus_tag="VFA02_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZU10295.1"
                     /translation="MSESTMIANSRIASVQELYPELKKIPAFSQISEQDLACLGDVEI
                     LDVPAGTVLLHPGDAPEHFWIILKGATRAHKTDKDGSMVFVSNAKEGDTFGEVPILAG
                     APKVFVTVETVKDSTLARIRQEHFWQLMATCPTVRNAVLANMSRRLEAHTALTLHREK
                     LISLGTLAAGLMHELNNPGTAARRAASQLRENMTRLQEISLRMTHTRLSDEQIQCLHQ
                     LLEEAFAAQKKPQALNSLDEADAQDALAEWLEGIGVTNAWKLAPTLVAAGWEKQDIEC
                     TQHAFPPDALSDALNWLEAVISNIQLVGTIEESIARVTDLVMAVKKYA"
BASE COUNT         2496 a         3069 c         3207 g         2625 t
ORIGIN      
        1 agcggctgcc cattggtcgt gcccagcgcg acggcatcgc cttcattgag tccggagagg
       61 atttctatct gcgtaatatt ggatgcgcca gtcgtcacct gggtgcggcg aagcactcca
      121 tctactatgc gatagacata gaatttgccc tgctccgtat gcagggcgtc gcgcggcaca
      181 ttcaggacat tggccgaatc agatattgta acttttacag ttacatttgt attaggcaga
      241 agctggccat cggcatcatc aattgcaatc actgcctggc ccacgttgcg ggtctcctga
      301 tagcgaataa tcgtggaggg tacccgttca atatggccgt gccactcaag atctggcctg
      361 gcgtcccatg tgatctggac cggcatgccc tccttcagcc tgccgacctc tggttcgtcg
      421 aaataggcaa gtacctgcat ttttttgagg tcggccattt ccaggagcgt ctttccctgc
      481 tcaacaaatt ccgttgcttt aaaggggatg gagtatacgg tccctggaaa cggcgctttg
      541 acgttggctt gattcactac ctgcagggcc gccgcatagg ccgcctgcgc atcttcaagt
      601 gacgcttttg catgggccac atccgtggag ccgtaccggt cggtcttctt tttctggagc
      661 actgcaagcg aattgtttgc agctgcaagt cggtcctttg ccgctgctac ctctgaaggt
      721 gaggcggcac cctgcatctg gagtttttca agagtagcaa ggctgttctg tgcctgggcg
      781 cggtccatca tcgctgattg aatctgccca ttgagcgaaa gcaactcttc ctgtgtgcct
      841 ccgttgactg tggcatcata agccgcctgc gcgcctttga ggtttgccaa agctgtggca
      901 agcttagcac gagcatcagt gtcgtccatg gaaagcagca gtttgcccga gggtacctgg
      961 tccccttcat ggacataaat cgccttgata atgccgggga agggggctgt ggcttcatat
     1021 ctggaaacgg ggtccaccac gccgttggtg ctggtggtgc tcctcaggct gctgcgcaca
     1081 actgtcgcag cccgcacggg aagcgttgtg cgcgtgaggc ggtgcgcaat atagaagacg
     1141 agcagaagcg taatgattac gcctgtccag agccagccac ttcgaattgt tgccgtgcgt
     1201 tgtgttgcca tccgtaacca gaggtcagta tataagactc aataagatgc ggattggtgg
     1261 cagaaatgta ttctgcgggg tgctctaaaa taagaacatg gctctttctg aacgctatac
     1321 cgatgagcac gcaaaagcgg gcctcgatta taagtttccc gagattgaaa cctggaaaaa
     1381 ccagtttcct gcttacgaga ttgtgattga cgatccggag ttcacctcag tctgtccgaa
     1441 gactgggctg cccgatttcg gcgtcatcac gctgcgctac atgccgcgcg agctttgcct
     1501 tgagttgaag tcttacaagg agtatctctt ctgctaccgc aacctgggca ttttccagga
     1561 aaacgtggtg aaccaggtac tggaggatgt ggtgaaggcg tgcaaccctg tatgggcggt
     1621 tgtaaaagga gagttccggc cccggggcgg catttccaca acggtagagg cgcgctggcc
     1681 caggtcccgg gagtaaatgg ggctgcctga aaaccgggcc tgatatcctg ccttcaccag
     1741 aatgaaggcc aaagcgcaag ccagacctac cgccgcgcag accgccggaa tggcgctttt
     1801 tctgcttacg tgcctgaatc ttttcaattt cattgaccgc tacgttttgc ctggcgtgca
     1861 gccgctcatc cagaaagagt ttctggtcaa tgatgctcag attggcctgc tgacctcggc
     1921 cttctttttc acttacatga tcgtcgctcc gcttaccgga tggctcggcg accggtttcc
     1981 acggcgtccg ctgattgtaa ccggagcact tatctggagt gcggccacgc ttctgactgc
     2041 caccgtgcat agttacgaaa cactgctggt ccgtcatgcg gttgtgggta ttggagaggc
     2101 gaccttcagt attttcgccc cggcgctgct ctctgatttt tatccagaga tagaccgcaa
     2161 ccgcgtcctc agcatctttt acatcacgat tcccgtgggt gcggcactgg gctatctcac
     2221 aggaggggtt ctcggtcagc attatggctg gcgtgtgccg ttttttgtcg cagccctgcc
     2281 cggagtgctg attgccgcgg ccttctggtt ctttgtgaag gagccgccgc gcggctcctg
     2341 tgatgagcta aagccaacct ttgaccgcac aacgctctcc ggactgctgc gtaatcctgc
     2401 cttctggact gcgaccctcg gcatggccat gtggactttt gccgtcgggg gcatctcaac
     2461 gtttctgcca actttcttcg tccgttttgg cggatacact gtggccacgg caggcatagt
     2521 tacaggcgca atcaccgcga tagatggcct ggtgggcaca atcgccggag ggtggattgg
     2581 gcaccgttgg gtacgaaaaa accaccgcgc gctctatctt gtctctgcct ggagcatgat
     2641 gcttgccatt ccggcctgcg ctgcaactct ctttggtccg cgttcgtggc tggtgcccgg
     2701 agcatttgtt gcggagctgt tgcttttcct gaacaacggc ccactgaatg catgcctggt
     2761 caattccgtg gcggcctcga tccgctcctc cgccattgct atcaatctgt ttataattca
     2821 cgcgctgggg gacgcctttt ctccccgcct gattggccgc gtttccgatg cgacaacttc
     2881 tctgcgcacg ggaatgggaa tgacgctgat tgctttgggg attgcgtcgg cgattttgtt
     2941 taccggatcg cgctttgcgc cgttgttgcc ggaaagcgaa gatgtttctt tctaaggcac
     3001 attgaggcat ttttcgtttc actgcgatgc catttcgtgc ggcgcaggat ctcagaagga
     3061 ttggccgggc ttacatcgct aaggtccttc ctcccctgcg gtcgtctttt caaggtgtga
     3121 ggtacttggg tttacagagg gtgaggcgca tcagggtgct acatgcctac gctcatgcgc
     3181 gttcttgggt cgaggtagtc gcgcagggca tcgcccagaa aattgaatga gagcacgcac
     3241 aacatgactg caattgcagg aaaaatgacc aggtgcggcg catcgaagag atgcgagcgt
     3301 gcgtcgttta acatcgcgcc ccaacttgcc gccgggggtg ggacaccaag tccgaggaaa
     3361 ctcagtgtcg cttcagccag cacggttccg gccaggccaa tggcggcctg aacgatcaca
     3421 ggctgcagga tgttcggcag gatgtgcttt gtaaagatgc gcaggtcgct tgcgccgagc
     3481 gcccgtgccg cttccacaaa ctcgcgctcg cgcgcagcca gcacctgtgc ccggacgagc
     3541 cgcgcataac ctacccatcc gccgatggag agggccagaa tcaaattgat tagcccaggc
     3601 ccgaggaatg cgacaaaggc aatcgccagc aaaattccag gcagcgccag gaaggcattc
     3661 atcagaaaag tattgagcgc agtatccaga acgccgccgt aatatccggc gatgccgcca
     3721 aggaccagtc ccaaaaacag cgagcaggcc acgaccgaag acgccacgaa cagcgaaagt
     3781 cttgcgccat aaagagtgcg ggaaaagatg tcgcgtccca gctcgtccgt tccaaaccaa
     3841 tgtgtatgat caggtggcgt aagcctgtgt tccagattta tggcggcagg attgtaaggc
     3901 gctatcaatg gcgcgaagag cgcaattcca acaaagagaa cgagcagcgc tgcaccgatg
     3961 acggcaagcg gctgccggcg aaatgtgcgt cgaatggaaa agtcggccaa agaaaaagag
     4021 tagcaaatgc cggggatttg ctttctttat acactcgaga ttcggcagag atccgtccct
     4081 gcttttccac agagaaatct atccggacgg atttatacgt tgacacgccg gtactgtgac
     4141 ccgcttaaat taaataaaag aaaagacctc tacaaaaaaa gattgccgca attttgcacg
     4201 acgttcaact tcctcagaca aagtaagaaa aagcgcagat tgcgcacaaa ggacaaaaca
     4261 tgaagactat tttgaggctg gtgttgcttg cagtgtttct ctgtccctgt accgctgccc
     4321 tggcgcagac tgccggggcc gggaccatca gcggaacggt gacggacaca ggtggcgcag
     4381 ttatcggcaa tgcaactgtt acggttacaa atactgacac cggggcagcc cgctccatca
     4441 caacaaacaa tgccggcagc tatgtggctc cttttctgca accaggacat tactccattt
     4501 cagtgagcgc gccgaatttt ggcaatgtgg aacagaagaa cattacactc catgtaggcg
     4561 atacgctgac agtggatgcg gcgctgcctc cggcagcggt acagtccacg gtgcaggttt
     4621 ctgccgcggc gcctctgctt gatccagagc ggacagaggt ctctcaggtt gtcagtgaaa
     4681 agctggtgca aaacctgccg gtgaatggcc gccgatggga cgatttcgtc ctgctcacgc
     4741 cgaatgtcgt caatgatgga ggcagcggtc tggtcagctt ccacggcatc tccggtttgt
     4801 ataacagcaa ctatgtggat ggcgcgaaca ataaccagat gctgttttcc gaggcacgcg
     4861 gtcgggcatc cggagcacct tacatctata gcctggactc cattaaggaa tttcaggtgg
     4921 cgacggccac gtacagcgca gagttcgggc aggctgccgg cggacaggtc aacgcaatca
     4981 ccaagtcggg cacgaacgat ctgcatggcg atctttttta ctatctacgt tatccatcgc
     5041 tgaatgcgct cgatccttat aacaaggggc agggaattgc gaaccataat ccgctcctct
     5101 tgacccagcc gattcaccag cagcagcagt ttgggggcag cgcaggagga ccgattgtcc
     5161 gcgaccgcct cttcggcttc ttcacgtatg acgggtttcg ccgcgtcaat ccggtgctct
     5221 atagctcgac agcaaatatt tcgctgactc cggcaggctc ttttggcgac cacggaaaca
     5281 acattacgcc tgcgcagtgt cctgcgagca ttggcggccc gggttcagcg aactgcacaa
     5341 atgcgatcaa atatctgcag gcgctctctg gttcttatcc gcgcaccggc gtgcaggaca
     5401 tcttctttcc gcgccttgac tggcagatga acgacagaaa ccatgtcttt gccaatttca
     5461 atttctctaa ctatcactcg cccagcagct atgcctcgag tccttctttt acaaattctt
     5521 cagtcacgac caatgggtct accaattatc acgagcgttt tgtggttgcg cagtggaccg
     5581 acgccctgac agaccattcc gtgaatgaag tgcgcttcca gtgggggcgc gatctggaaa
     5641 cagcgggtgc aaatgcgcct gcccccagcg tttccctggg cggtgtgcag acttacggaa
     5701 tgccgaatgc gcttccgcgc acggcagagc cggacgagca ccgttatcag atcaccgatg
     5761 tgttctcgac tacacatggc agacacactc ttaaagccgg aggcgatctg aatcttgttc
     5821 atgaagtcat gatcaatctc tttcagggag gcggtctgta tagctacacc aaaggcgtcg
     5881 gtccatccga caattttgct gcctgggtgc aggacttttt tcccgcaact tcttccgacc
     5941 cgcttgccgg gacgcattat gacaccttcg ttcaggtgat agatccgatt acgcacacag
     6001 gcaaggacga tttctggatg aagatgattg acggctttgt cgaagactcc tggaagatta
     6061 cgcacgacct tacgtttgat cttggagtgc gttacgatgt gcagctcacg cctcaacctc
     6121 tgcatccgaa cacgagcact gctttagcgg cccattacaa ccagacactg aaaaacgttc
     6181 tcgaccgtgt gcagccgcgc attggttttg catggaaccc aatacagggg accgtggtgc
     6241 gaggcggtta tggtctattc agcgcgctga atcagggcag cacctattat gcgatgcgcg
     6301 tggaaaacgg tgtcttccag acgaactata gcttcgtggg tgccggcgga gcaggaggaa
     6361 agctgccggt tggggcgccg ttgtttccca atgtgctatt tacgcctccc ggaccaggtc
     6421 tgaactcggc tccactgtat ggcgcgccca gtccaatcag ctatgggggc gcggccagca
     6481 cagtgacccc tctgccatcc ggcttaaagc ctgtgccaag ctttcacggt cttgatccga
     6541 actttgtgcc gccgctggca cacgaaatgc agctcggcgt ggaacagcag atgcccggcc
     6601 gtatgacgct ctctattggt tacgtcggca cgcgcgctct tcgccttcct gtttttgtgg
     6661 atggcaacct gattggccag aagccgagcg gtacaaagac atatcaacag gtagataaga
     6721 gcggaaagcc cattggcaag ccgtttacgc tgccgttcta cctgcctacc gaccgccgcg
     6781 atactaccat cggatcgctg aataccggct tcagttccat caatacttgg tacaactcca
     6841 tggcggtcac gattaagaag ccattcgagc atggtctgga actgcttgtg aattacacct
     6901 gggcaaaggc gctcgatctg gggcaggtac agggggccta cggcacgttc tacggctctg
     6961 attttccgct ggatccaaac gatctgcggg ccgaatatgg acgttcggat cttgatcagc
     7021 gcaaccggtt catcggcagt ctcgtttatc agccgcagtt gtatcaaagc aatcgctggg
     7081 taaggcacct gctggatgat ttcatttttt ccggcacggc cactgaaacc accggctttc
     7141 ctgtgacggc cacgctgagc ggttatcctt caggcggggc agatggcggt gtcacgggcg
     7201 gggtgatgag ttccagttcg ggaacggcaa ccaatgggcg tgtccccaac caggtcgccg
     7261 gccgcaacgc ttttcctgga cccggcgtgc gcaatattga cttccgagtg acaaggaatt
     7321 ttccaattac tgagaagatc aattttcaga tattggccga agcatttaac ctgctgaacc
     7381 agcgtattaa tcagtctgtg aatacaacag gatactcact gctgacccca ggaaaagcag
     7441 gggcctgcca gagctactca ggcagcgcca taaccggagg ttgcattgct cccactacgg
     7501 gagtgccgct gggtacacct acctcaacca gtagccttct ctacggagcg agacagttgc
     7561 aattttccac aaagctgttt ttctgaagaa gttcaggccc aaagcaaaaa ggcacgattt
     7621 tcaaggaaga tcgtgccttc acttttggac agaacaagtg ccgggcgtta acctgcctgc
     7681 atcatcattt cgtttccgac tgcttcggcg gccgcggcga gcattttgtg gtgaatggta
     7741 aagagggcga gttccaggcg gtcagaaact ccggtcttgt cataaatgct gcggagatag
     7801 ttcttcacta cctgttcggt tgtccccagg cgcatggcga tctctttatt cttgcagcca
     7861 tgaataatga gagcgacgat tctcatttcc tttggtgtga ggcggtcacg gacgcgcgcg
     7921 ccgacctcgt cttcattttg cgtacgccgt aaaacctcaa gcgcaggttg cagatattgc
     7981 tcacccattg ccactctgcg gacacaatca ataagagtgg agccgctgac agaacggtaa
     8041 agaatcccat gaaccccatg ctccagccag tgccggggag attcctggtt ttcaagaacc
     8101 acgatagaac ggcagcccga ctttgctact cgctccatca ggaggggcaa atcgagattc
     8161 agtgcggaag taaataaaag gactgtatta cggaaggaat caagtgcctg gtatagccgg
     8221 tcgctatcag cacattgagc aataattcgg aaatcatctt ccatcgccag gattttggct
     8281 gatcccgcgc gatagacggc ttgattgtcg gccagaataa ctttgaacat acgagcgtgt
     8341 cgtcccccga taaccgctct gcgccatagt aatatgcgca ggttaaaaaa gcatatgttt
     8401 gtgtgcaacg ttggatactt cccgttccac aaggcagaaa aattggtgtt ctctgaaaac
     8461 gcttcatggt gctttcagga aacttaagta cctatttgtt tgataagtga acatagctta
     8521 cataaaagtc ctgttttggg aaagagattt cgatggttat tttggggttc ctttggttat
     8581 ctgttctgct tttatctttc tggtgtaaaa ctacatgtct tcagaaaacc atgtggtggt
     8641 ccggtctggg gtcgttgact gccttgtccg tatgtggatg ggacgtccag gttttacctg
     8701 aacctatttt gtgcgtcgtc gcgccgcttt tggttctgcg caaaaacgac ttacactaga
     8761 agcgcatggc aaaaccggtc attcttgcgg tagatgacga catcagcgtt cttgaagctg
     8821 tgatccagga ccttcgccgc cagtatgggg ccaattaccg tatcctgcgc gccggttccg
     8881 gacaggcagc cttggatacg tgtacgcaat tgaagcagcg gggcgatgca gtcgcgttat
     8941 tcctttccga ccagcgtatg ccggggatga gcggtgtgga gttcctcgaa aaggcgcagg
     9001 ccatctatcc cgaagcaagg cgcgcgctgc ttaccgccta tgcggacacg gaagcggcga
     9061 ttaaagccat caacacggcg aagatcaact attacctcac gaagccctgg gaccctccgg
     9121 aagagaagct gtacccggta ttggatgacc tgctcgaaaa ctggaaggag ggttacaagc
     9181 ctccatttga gggcttgcgt gtcgtagggc cgcgctggtc ggcccgcgac catgaggtgc
     9241 gcgattttct ttcgcggaat caggtcccat atgtctggct tgatccggaa cggaacgacg
     9301 aggcggtgca acttctggag caataccaga tcaatgacca gaagctacct gccgttctct
     9361 ttccggacgg aagctcgctg gtccagccca gccgaatcga actggcggcg cgtattggat
     9421 tgcgtacgca ggccgagaag gacttttacg atgtcgccat tattggcgcg gggccggcgg
     9481 gtctggcagc cgcggtttat gccgcttctg aaggattgaa ggcgattgtg gttgagcctg
     9541 atgctcctgg cggacaggcc ggaagcagtt cccgcattga aaactacctt ggattcccat
     9601 ccggtctgag cggggcagat ctggcccgcc gtgcctacgc ccaggcatcg cgatttgggg
     9661 cagaatttct gacccagcgg gcgactggca tccgcaccga ggcgccatac cactttgtcc
     9721 agttggccga tgggcgggag atatcctgcc acattgtgct tgtgtccacg ggtgtggact
     9781 accgcaggct cagtatccct gggatcgagc agtttacggg agcgggggtc tattatggag
     9841 cggcgctgac cgaggccctt tcctgcaagg acgaggacat atacatcgta gggggcgcca
     9901 attccgccgg gcaggcggcc gtccacttcg cccggtatgc ccgcaaggtt acgatgcttg
     9961 tacgggcgga ctcgctcgaa aagagcatgt caaaatattt aatcaatcag atagctggca
    10021 catccaatat tgaggtgcaa actggcgctg aagtgcagga agttgcaggg aatgggcgtc
    10081 tggagtgcct gaaagtgcga aaccggcatg gggagctggc ctgtcaggcc agtgcgctgt
    10141 ttatctttat cggcgccgaa ccaaagacag aatggctgcc ggaaaacgtt atgcgggacc
    10201 cccgggggtt cgtgctttct ggcccagatc tgctgattga gggaaagatg cctaaaagct
    10261 ggaaagaaga ccgtccacca ttcctgctgg aaaccagcac accggggatt tttgtcgctg
    10321 gcgatgtccg gcacggttcg gtaaagcgag tggcttcggc agtcggcgaa ggctcgattt
    10381 ccgtgcagtt tatgcatcag tatcttgcgc gtttttagga tttaaatgtc agaatcaacc
    10441 atgatcgcca attcacgaat agcctccgtc caggagcttt atcctgaact gaagaagatt
    10501 cctgcgtttt cacagatctc ggaacaggac ctggcttgtc ttggcgacgt tgaaattctg
    10561 gatgtgcctg ccggcaccgt gttgctgcac cccggagatg ctcctgaaca tttctggatc
    10621 attctgaaag gcgcaacccg cgcgcacaaa accgacaaag acgggtcaat ggttttcgtt
    10681 tccaatgcca aagaaggcga tacctttggc gaggtcccga ttctggccgg tgctcccaaa
    10741 gtctttgtga cggttgaaac tgtcaaggac agcacattgg cgcgtatccg gcaggagcac
    10801 ttctggcaac tgatggcgac ttgcccaacg gtgcggaatg ctgttctggc aaatatgtcg
    10861 cgccgcctgg aagcgcatac ggcactcact ctgcaccgtg aaaagctgat ttcgctggga
    10921 acactggctg ccggactgat gcacgagctg aataatccgg gaacggcggc cagacgtgcg
    10981 gcttcgcagt tgcgggaaaa catgacccgg ctgcaggaaa ttagcctgcg catgacgcat
    11041 acccgcctca gcgatgagca gattcaatgc ctgcaccaat tgcttgaaga agcctttgct
    11101 gcccagaaaa agccgcaggc gctgaactcg ctggatgaag cagatgcgca ggacgccctg
    11161 gccgagtggc tggaggggat tggcgtaacg aatgcgtgga agctggcccc tacactggtt
    11221 gcggcaggat gggaaaagca ggacattgaa tgcacgcagc acgccttccc tccagacgcc
    11281 ctttcagacg cgctgaactg gctcgaagca gtcatctcca acatccagct cgtgggcaca
    11341 atcgaagaga gcattgcacg agttactgat ctggtgatgg cggtcaaaaa atacgct
//
LOCUS       DASRBV010000281         6846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_528986, whole genome shotgun sequence.
ACCESSION   DASRBV010000281 DASRBV010000000
VERSION     DASRBV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528986"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFA02_10375"
     CDS             complement(<1..248)
                     /locus_tag="VFA02_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coatomer subunit epsilon"
                     /protein_id="HZU10296.1"
                     /translation="MDTQTRHALKQDSFVQATASGLTWLDEHRATVIRVSIAVVVLAG
                     LFFAGLITYNNRSERAASLFGQAMDIYSTPIAQPGQPA"
     gene            complement(390..2576)
                     /locus_tag="VFA02_10380"
     CDS             complement(390..2576)
                     /locus_tag="VFA02_10380"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZU10297.1"
                     /translation="MAVKEMPHSDVIEIGNQFYIRAQSSLADSRTLVLMHRDTFAVFD
                     RNGDVLPLGVGQQGIFHNETRYLSRMEMRICGRRPLLLSSNTSEDNVLLAIDLTNPDT
                     ELGPGEFLPRGSVHIYRAKFLADGTCFDRITVHNYGQKVVHIELSFAFDSDFADIFEV
                     RGQRRKRRGAYLPPEIDQSGIALAYEGLDDVLRRTRITCTAVSCVMGDKHMTIPLQLQ
                     PQEETSFLLDIQCLSNGTARAGADYDSVLQERSTSRQTSSLAGIDIFTSNEQFNDWLN
                     RSKADLEMLTAETPFGPYPYAGVPWFSTVFGRDGIITALETLWLAPGIARGVLSYLAA
                     TQAASNDPERDAQPGKILHETRKSEMAQLREVPFGRYYGSVDSTPLFVLLAAAYYERT
                     NDAGFLREIWPNIEAALHWIDNYGDVDGDGFVEYARQNENGLLQQGWKDSQDSVFHAN
                     GKVAEGPIALCEVQSYVYAAKSRIASVAEDLGKLNLAEQLKKQAEELRARFTPAFWCD
                     ELSLFAIALDGGKKQCRIRSSNAGHCLFSGIASAAHARSVADSLLSPTFFSGWGVRTI
                     ATGEKRYNPMSYHNGSVWPHDNALIAFGVSGSPSKDLAIKVLSGLLDLSLFVDMHRLP
                     ELICGFPRRPGRGPTLYPVACSPQAWAAGSIFMVLQACLGLSVNAKESRIYLRHAALP
                     ESLERIRIRGLQIGNASVDLALERQAQSVGVDILHRSGKIEVVSIK"
     gene            complement(2604..3677)
                     /locus_tag="VFA02_10385"
     CDS             complement(2604..3677)
                     /locus_tag="VFA02_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU10298.1"
                     /translation="MKIAQVAPLYESVPPKLYGGTERVVSYLTEELVRQGHEVVLFAS
                     GDSQTNAELRAICPRSLRLEGKKVIDPLAHHVRMLEMVAKEAEEFDIIHYHVDYLHFP
                     LSRRLGVPTVTTLHGRLDIPDIHPLLREFGEMPLVSISDAQRTPVKWANWVATVHHGL
                     PEDLYKPIAKPGKYLAFLGRISPEKRVDRAIEIAKQAGMPLKIAAKVDRADKEYFDGK
                     IRKLLDHPPVEYIGEIGEKEKNEFLGNALALLMPIDWPEPFGLVMIEAMACGTPVIAW
                     GMGAVPEVLDDGITGYIVSSMDEAVAAVERVAVLDRVQCREAFEKRFSARRMGCDYLS
                     VYEKLCGREPELTIVGKTFSVAA"
     gene            complement(3934..4320)
                     /locus_tag="VFA02_10390"
     CDS             complement(3934..4320)
                     /locus_tag="VFA02_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZU10299.1"
                     /translation="MTLSPQNLRRLEEKLRQQARAIERSMLTAVEQGRQTLADETQDA
                     ADLAVTAYQKEMLFTQGTSGAGQLSLIRLALERLREGTYGECIECGNMIGEKRLEALP
                     WTPYCIHCQEKIEQGQLEPVARRRVA"
     gene            4515..4910
                     /locus_tag="VFA02_10395"
     CDS             4515..4910
                     /locus_tag="VFA02_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10300.1"
                     /translation="MYEDFHVTDRWTGEDLHVRWKANIVAIATRHADAVDIRFDVNGR
                     PVWIAMPCQAWVEQKKRTGHVITDQLAAQIAGLYLKRAIESGYDNGREMYTMSVDEVL
                     ELLDVVLREAGHTSKLPTIPATSDAQPVP"
     gene            5018..>6846
                     /locus_tag="VFA02_10400"
     CDS             5018..>6846
                     /locus_tag="VFA02_10400"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HZU10301.1"
                     /translation="MRHVKLLLAAAVLLMYTHTFAQTASIHFNPQTRVFRLDGGSISY
                     AFGINNADELQPLYWGARLNTGDPLGPAHTNMGHASFDLPSGSTLQEFPGWGEGLYYE
                     PSLKITFPDGNRDLVLHYIDHSIHGNTLTIRLKDIEREVFVELKYQIDPKTGILARSA
                     VITNKTNSPFMIEQAAAAAWSLPNSKDYWLYYLTGRWAGEWNVQHEAVRPGARVLESR
                     RGSTGHQNNPWFAIERGDNNDQDTGDVWFGALAWSGSWRITIEQDILNQVRITGGYNP
                     FDFGYKLAPGEQLVTPVFYGGFTEHGIGGASRLLHQFELANVLPQAPKPRPRPVLYNS
                     WEATEFNVTEQGQEALAEKAAKIGVERFVVDDGWFGQRKDDHAGLGDWYVNKQKFPHG
                     LKPLIDKVHSLGMDFGLWVEPEMVNPDSDLYRRHPDWVLNFTGRPRTEGRNQLVLNLA
                     RQDVRDYVFGFLDKLLTENDIAFLKWDYNRNWAEPGWPAVAPDEQKKVYVKYIQNLYS
                     ILEELRAKHPNVEIESCSGGGGRVDLGIMHFTDEVWTSDNTDPFDRLSIQNGFTYAYT
                     PGVMMAWVTDSPNWVNRRTTSLEYRFLSSMQGSLGVGANLNNWT"
BASE COUNT         1507 a         2014 c         1889 g         1436 t
ORIGIN      
        1 tcggcgggct gaccgggctg ggcgatcgga gtgctgtaga tgtccatggc ctgtccaaaa
       61 aggctggcag cgcgctcaga acggttgtta taggtaatca gtccggcaaa aaagaggccg
      121 gcaagaacca caaccgcaat cgaaacacga atgaccgtgg cccggtgctc atcgagccat
      181 gtaagaccgg aagctgtcgc ctgaacgaag ctgtcctgtt taagcgcgtg acgtgtctgt
      241 gtatccacta ggtccttaga aatccttctg ggcaggagaa attacaggca atctcaggaa
      301 aagggctgcc tgtctggata ccaaaagtct atcagcggga aattcaaggg gtcaaacaac
      361 agatttccca aagccaggcg tcggggcatt cactttatgc tcacaacctc aatcttcccg
      421 ctgcggtgca ggatgtccac gcctacactc tgtgcctggc gctccagtgc gaggtctact
      481 gaggcattgc caatctgcag gccgcgaata cggatgcgtt ccagagattc cggcagtgcg
      541 gcgtgtctca gatagatacg cgactccttc gcgtttacag acaggcccag gcaggcctgg
      601 agcaccataa aaatcgagcc tgccgcccat gcctgcggag aacatgccac aggatacaga
      661 gtcggccctc tgcccggacg gcgcgggaat ccgcagatca actccggcag gcggtgcatg
      721 tccacgaaca gagacagatc aagcaggcct gagaggacct ttatggccag gtccttgctc
      781 ggactgccgg aaacaccaaa ggcaatcagg gcattgtcat gcggccacac cgatccattg
      841 tggtacgaca tggggttata acgcttttct cccgtggcga tggtgcgtac gccccaaccg
      901 gagaaaaatg taggagagag cagggaatcg gccacgctgc gtgcatgggc tgccgatgca
      961 atgccggaaa acaggcaatg tccggcattc gagctgcgga tgcggcattg tttcttgccg
     1021 ccatcgagtg cgatggcaaa caacgaaagc tcatcgcacc agaatgcggg agtaaagcgg
     1081 gcacgcagtt cttccgcctg ctttttcagt tgctccgcca ggttgagctt acccaggtct
     1141 tcggccacgc tggcaatacg cgatttcgca gcatacacat acgactgcac ttcacacaga
     1201 gcgatggggc cttcggcgac cttgccattg gcgtgaaaga ccgagtcctg ggaatctttc
     1261 cagccctgtt gcaaaaggcc gttttcattc tggcgcgcat actctacaaa gccatcgcca
     1321 tctacgtccc catagttgtc aatccagtgg agagcggcct cgatattggg ccatatctcg
     1381 cgcagaaaac ccgcgtcgtt tgttcgctcg tagtatgccg ctgccagaag aacaaagagc
     1441 ggggtggaat ccacgctgcc gtagtagcgt ccgaaaggga cctcgcgcag ttgcgccatc
     1501 tcgcttttgc gcgtctcgtg caggattttc ccaggctgcg catcgcgttc ggggtcgtta
     1561 ctggccgcct gtgtcgccgc cagatacgag agcacgcccc ttgcaatgcc cggagcaagc
     1621 caaagagttt ccagtgccgt gataatgccg tcgcgcccaa agaccgtgct gaaccagggc
     1681 acaccggcat agggatacgg cccgaacggg gtttcggccg tcagcatttc caggtcggcc
     1741 tttgagcggt taagccagtc attgaattgc tcgttggaag tgaagatgtc tatgccggca
     1801 agggatgagg tctgccgcga ggtgcttctc tcctgtagta ccgaatcgta atccgccccg
     1861 gctcttgcag ttccattact taagcactgg atgtccagta gaaagctggt ttcttcctgc
     1921 ggttgaagct ggagcgggat ggtcatgtgc ttgtcgccca tgacgcagga gactgccgta
     1981 caggtgatgc gggtgcgccg cagcacatca tccaaccctt cataggccaa cgcgatgccg
     2041 gactggtcta tctccggagg aagataggcc ccgcgccgct tgcgtctttg cccgcgcacc
     2101 tcgaagatat cggcgaagtc tgaatcaaac gcaaacgaaa gctcaatatg aaccaccttc
     2161 tggccgtagt tgtggaccgt gatgcggtca aaacaggtgc catcggccag aaattttgcg
     2221 cggtagatgt ggacggatcc gcggggcaga aactctcctg gccccagttc cgtgtccgga
     2281 ttggtcaggt caatcgcgag caggacattg tcttcactgg tattggagct gagcagcaag
     2341 gggcgtctgc cgcagatgcg catctccatt cgggaaaggt atcgcgtttc attgtggaag
     2401 atgccttgct gtcccacgcc gagaggcaga acatctccat tgcggtcaaa gacagcaaag
     2461 gtatcacggt gcatgagcac cagcgtccgg ctgtccgcca gcgatgactg ggcccggatg
     2521 taaaactgat tgcctatctc gatgacgtca gagtgaggca tttccttgac tgccatgagg
     2581 ccatacatct cccggaatga agattacgca gcaacggaga atgttttgcc cacaattgta
     2641 agttccggct cccggccgca gagcttttca taaacactca gataatcaca gcccatacgg
     2701 cgtgcactga accgtttttc aaaggcctcg cggcactgca cacggtcgag taccgccacg
     2761 cgctccacgg cagccacagc ttcgtccatg gaagaaacga tatagcccgt aatgccatcg
     2821 tcgagcactt cgggaacagc tcccataccc caggcaatca ccggggtacc gcaggccatc
     2881 gcttcaatca tgaccagacc gaatggctcg ggccagtcta tcggcatcag gagtgcgagg
     2941 gcgttgccaa gaaattcatt tttctctttt tcgccgattt cgccaatgta ttccaccggt
     3001 ggatggtcga gaagcttgcg gatcttccca tcgaaatact ctttgtccgc gcggtctacc
     3061 tttgcggcga ttttcagcgg catacctgcc tgtttcgcaa tctcaatggc gcggtctacg
     3121 cgcttttccg gagaaattct gcccagaaaa gccagatatt tccccggttt cgctatgggt
     3181 ttgtagaggt cttccggcag gccatgatgc actgtggcca cccaattggc ccatttcacc
     3241 ggggttcgct gcgcatctga aatagagacc agtggcattt cgccaaattc gcggagcagt
     3301 ggatggatgt ccggaatatc cagccgccca tgcagagtgg tcacggtggg aacaccgaga
     3361 cgccggctta gtggaaagtg cagataatcc acgtgatagt gaatgatatc gaattcttcg
     3421 gcctctttgg ccaccatctc cagcattcgg acatggtgcg ccagagggtc aatcaccttt
     3481 ttcccttcca ggcgcagaga acgcgggcag atggcgcgca gttctgcgtt ggtctgcgaa
     3541 tcgccgctgg caaagagcac cacttcatgc ccttgccgga cgagttcttc cgtcaggtag
     3601 gacacaactc gctccgtccc gccataaagt tttggaggca cactttcata aagaggagcg
     3661 acctgggcaa tcttcatctc agtcttctcc aacttgttac aaaaattttc tgcttgcccg
     3721 cgaatggccc ctggagcaga accggcagtc atgcgcaaca gaaaagcgct gatgaataag
     3781 atgtcgcggt ctgcccagtg gctttgcagg ctcgctcatt caactgcaaa cctgtggtaa
     3841 ggctgcaatc ccgcgcagat agagatgtgc gctgtcagta caaaaaatag atgaaaactt
     3901 ccgaaacaaa agttgcccgg ccgggggcgc cgcctatgct acccggcggc gcgcgacggg
     3961 ctccagttgg ccctgttcga ttttttcctg gcaatgaata cagtaagggg tccagggaag
     4021 cgcttcgagc cgcttttccc caatcatgtt gccgcactcg atgcattcgc cataggtgcc
     4081 ttcgcgcagc cgttcgagcg ccagccggat gagcgacaat tgtcctgcgc cgctggttcc
     4141 ttgcgtgaag agcatttcct tctggtaagc ggtcactgcc aggtcggcag catcctgtgt
     4201 ttcatcggcc aatgtctgcc ggccctgttc caccgccgta agcatggaac gctcaatcgc
     4261 cctggcctgt tgccgcagct tttcttcaag acgccgtaga ttttgtgggg aaagtgtcac
     4321 gtttgtgcgc tccttcgtta catcagattc cgttcacttg cgtgtcaaat attggacgca
     4381 cgagatacgt aaagatttgt tccgggttgt gcgaatttcg caatttttgc agccccctgg
     4441 cgtcctgcat cggtgtattt cggcgcgagc gggaagatgc agcagccctc gcccttgata
     4501 cactgaaatc agccatgtac gaagatttcc acgtaactga ccgctggacc ggcgaagacc
     4561 tccatgtccg ctggaaggcc aacattgtcg ccatcgctac gcgccatgcc gatgcggtag
     4621 acatccggtt tgatgtgaac ggaaggcccg tctggattgc catgccctgt caggcatggg
     4681 tggagcagaa aaagcgcacc gggcatgtga ttaccgacca gctcgccgcc cagattgccg
     4741 gactgtatct caagcgggcc attgaatcgg gatacgacaa tggccgcgaa atgtacacca
     4801 tgagcgtgga tgaagtcttg gagcttctgg acgtagtgct gcgcgaagcc ggacatacca
     4861 gcaagctgcc aaccatcccc gctacaagcg acgcccagcc ggttccataa caaaagagat
     4921 acaagcacgg ccagaaatag cagcacggat gacaccctcc tcccgttccg tatatgttta
     4981 cctttgaaga tttctgcggg ggagttcctt catccaaatg agacatgtaa agctgcttct
     5041 ggctgctgct gttttgctga tgtacacgca tacattcgca cagaccgctt ctattcactt
     5101 caatccgcag acgcgcgtct tccgtctcga tggcggaagt atctcctatg ccttcggcat
     5161 caataacgcc gatgaattgc agccgctcta ctggggcgcg cgcctgaaca ccggcgaccc
     5221 tctcggccct gcccacacca acatgggcca cgcttcattt gatctgccca gcggcagcac
     5281 tttgcaggag tttcccggat ggggcgaagg cctgtactac gagccttcgc tcaaaatcac
     5341 cttccctgac ggcaaccgcg acctcgtcct gcattacata gaccacagca tccacggcaa
     5401 cacactgacc atccgcctga aggacatcga gcgcgaagtg ttcgttgagc tgaagtacca
     5461 gatagaccca aaaacaggaa tcctggcccg ctccgctgtc atcaccaaca agaccaactc
     5521 ccctttcatg atcgaacagg ctgccgctgc tgcatggtcg ctccccaaca gcaaagatta
     5581 ctggctgtat tacctcaccg gccggtgggc gggtgagtgg aacgtccagc acgaggccgt
     5641 ccgtccgggc gcgcgcgtgc ttgagagccg tcgcggctcg accggacacc agaacaaccc
     5701 gtggttcgcc attgagcgcg gtgacaacaa cgaccaggac acaggcgacg tctggtttgg
     5761 cgcgctcgcc tggagcggct cctggcgcat caccatcgag caggacatcc ttaaccaggt
     5821 gcgcatcacc ggaggctaca atcccttcga cttcggatac aaactcgctc ccggcgagca
     5881 gcttgtaacc cctgtcttct atggaggctt caccgaacac ggcatcggcg gagcatcgcg
     5941 tctgctgcat cagttcgagc tggcgaatgt tctgccccag gcaccgaagc cgcgtccgcg
     6001 tcctgtgctc tacaactctt gggaagcgac agagttcaac gtgaccgaac agggtcagga
     6061 agcgctcgct gaaaaagccg caaaaatcgg cgtcgaacgt tttgtcgtag acgacggctg
     6121 gttcggccag cgcaaagacg atcacgccgg gcttggcgac tggtatgtga acaagcagaa
     6181 gttcccccat ggtctcaagc cactgattga taaggtgcat tctctcggaa tggacttcgg
     6241 cctctgggtc gaaccggaga tggtcaatcc ggacagcgat ctttaccgca ggcatccgga
     6301 ctgggtgctg aacttcaccg ggcgtccacg tacggaaggc cgcaaccagc ttgtgctcaa
     6361 tcttgcccgg caggacgtgc gcgattacgt cttcggcttt ctcgacaagc tgcttacgga
     6421 aaacgacatc gcgttcctca agtgggacta taaccgcaac tgggccgagc caggttggcc
     6481 tgccgttgct cccgacgagc agaagaaggt ctacgtcaaa tacatccaga acctctactc
     6541 gatcctggaa gagctgcgcg cgaagcaccc aaacgttgag atcgagtcct gctccggcgg
     6601 cggaggccgc gtggacctcg gcatcatgca cttcaccgac gaggtctgga cctcagacaa
     6661 caccgacccc ttcgaccgcc tctccatcca gaacggcttt acctacgcct acacgcccgg
     6721 cgtcatgatg gcctgggtca ccgattctcc gaactgggtg aaccgccgca ccacttcgct
     6781 cgaataccgt tttctctcct cgatgcaggg ctcgctcggc gtcggcgcaa acctgaacaa
     6841 ctggac
//
LOCUS       DASRBV010000282        10158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_17292,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000282 DASRBV010000000
VERSION     DASRBV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17292"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..3128)
                     /locus_tag="VFA02_10405"
     CDS             complement(3..3128)
                     /locus_tag="VFA02_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU10302.1"
                     /translation="MRSLISAILKQRLVVVVVALILLGFGINAARHLSVDAFPDVTNV
                     QVQIATEAPGKSPEEVERFITIPIEIAMTGLPGLTDMRSLNKPGLSLITLVFEDGKGV
                     YFERQLVNERLSEVRDRMPEGVNPVLGPVSNALGEVYQYTLERPDDGKRPLTHDELVE
                     RRTIQDWVVRPLLRSIPGVAEINSTGGFVKQYETLVDPQKLRYYGLTIEDVYAALKRN
                     NENAGGGILPQHAEQYLIRSVGLIRGVDDIRNIVLKENAGTPVYVRDVADVRIGEEVR
                     YGAMVKGGYTEAVGGVVMMIAGGNAKEIVSKIKERVAEINNRNMIPGGLKIVPYYDRS
                     RLVDAAIHTVTEVLEEGIVLVVVVLFLYLGDLRSSLIVCSNLLLTPLLTFLIMNHVGL
                     SANLMSLGGLAIAIGLMVDGSVVVVENVFAKLSHGSQDSRLHVVLDAVTEVATPVIFG
                     VTIIVLVFLPLMTLEGTEGKMFAPLAYTIAIALLLSLVLSLSLSPVLSAFFLKGGSEK
                     DTRLVSLLRRPYDRILSWAMQNKKKSVTAVVLLFVASLCLFPFLGTSFIPEMQEGTLG
                     PNADRVPNISLDESIKMEMQMQRLMRDIPGVESVVSRLGRGESPADPAGPNEADVIAT
                     LTPVDKRPRTLTQEVIADKMRKALATIPGINLVMSQPISDRVDEMVTGVRADIAVKIF
                     GDDLDTLIEKAQQVAKVATSIRGTQDTRVDRVGGQQYLVININRAAIARYGINAADVN
                     DTIEMAVAGKSATEIYEGERRFQAIVRLPQQYRKSINDIKQIMISSPNGPRVPLEDLA
                     DVRVLEGPAQINRDMGKRRIVVAINVQDRDLGGYVAELQQKVNAQVPLPAGYFIQWGG
                     QFQNMQRAMRHLMIIVPITIGAIFFLLFLLFNSLRFAALIITVLPFASIGGVIGLFIS
                     GEYLSVPASVGFIALWGIAVLNGVVLVSYIRKLREGGMSQQDAIIEGTRLRFRPVMMT
                     ATVAALGLVPFLFAIGPGSEIQRPLAIVVIGGLVTSTLLTLVIVPCLYAFFEGKPQDV
                     ADVEMEHHLLS"
     gene            complement(3128..4258)
                     /locus_tag="VFA02_10410"
     CDS             complement(3128..4258)
                     /locus_tag="VFA02_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10303.1"
                     /translation="MTTALLGTWGCKHEAEASKTAGSALRDPLKVRITPELQEDLKTG
                     VPEWQEITTQEKVMARVETDASRVARIGSPVEGRITKVLVFEGQRVSRGQVLATLHSN
                     ALSDTQFAFIKAYSQEKLAEQSAQRAKQLVQSDVIGTAELQKREAELLQTSAEVAALR
                     AQLRGLGMSDNAIRQLETTRKLNSDYPVIASISGTVIERKVTVGQVVQPADLAFQIAD
                     LSSLWLVADVPEEHAASLHIGKTVIANIPSLPNATVNGKLSFVSPVVDPATRTVQARM
                     NLLNPTGEFKPEMLATMTFESQPEKKLTVPATAVVREENKDHVFIQTAQNEFLLREVE
                     LGPEVSDRRVVLRGLSPDEKIVLDGAFHLNNKRKQDLLKGGE"
     gene            complement(4306..5529)
                     /locus_tag="VFA02_10415"
     CDS             complement(4306..5529)
                     /locus_tag="VFA02_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU10304.1"
                     /translation="MILLLLVAILAPSLLFAQAVTPLTLEQALSLAEQNNPRLRAGEA
                     GVERAKAGIQSAKAYINPTFEFLAGNQSALPVRTPGVPGLLQHYSAGQTIEIPSERRT
                     RIHASRLEFLSSEYQLAGIRLAVIADVKRAFYDVLRRKQQVAYAKDNLALVDDLHRRV
                     SVEVQVGEKGKLELTRAEAELARARAAVNSAEVELSNAQAVLKAVIGASANDEFDPQG
                     SLGNRAHLAPLDELRQRVFATHPALSEAKTETARAETLVKNERARRIPQPTFYGEYER
                     QPDISFYRFGVNVPLPLWDRRKGPIAEAQAEVKRSQAITRQRELEILAALERAYDQYQ
                     ISDQQVLGLQAGSLREAEAAVDAARSAYKFGERGILEVLDAQRVLQGVRTDLLDAEFA
                     RQSALIDLQELGATP"
     gene            5839..6951
                     /locus_tag="VFA02_10420"
     CDS             5839..6951
                     /locus_tag="VFA02_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU10305.1"
                     /translation="MGWSPRLWLERSAPGVALIVLLLLCFAALTLVVPGNDLWAHNIL
                     HHLNFLPLMIAGMLFGWRGAAWTALLAGFVNAPLIAHHWHRWPVDAKDQIVELSIFSA
                     AGLIAGYLSDRERAQRRNLEQTKRELEKVYTELRENVAHMKKAERLSAAGQLAASLAH
                     EIRNPLASISGAAGILRRGSASPEYMQDSLDIIDKESQRLNKLLTGFLNFAKPRSPRL
                     QKTDPNAVLASVASLASHAAQASHVALVHKPLENCPEIECDPEQLKQVLLNLVINAVE
                     ASPAGATVVLRTVYNSGPITLEVEDQGSGIHEDVADQIFDPFFTTKPKGTGLGLAVSS
                     MILSQHGGTLSFSKNTAGGTTFRVELPLHGDAARAR"
     gene            6941..8326
                     /locus_tag="VFA02_10425"
     CDS             6941..8326
                     /locus_tag="VFA02_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU10306.1"
                     /translation="MQGNLILIVDDDSSVRRVMQMQLAEAGYDVLPAATGSEAHRLLI
                     ERRPKLVITDLRMPDLDGIELLRRIAESEIQTTVIMITAFGSIETAVQAMRLGAYDYI
                     TKPIDYEALTLAVHRAMERQNLIDEVRNLRSALDQRYGFENIVGHSKSLLRVLEMASR
                     VAQHDTTVLVNGETGTGKELLARAVHHNSRRRNQPFVTINCGAIPKDLIEAELFGYAR
                     GAFTGASNSKPGKVEMADGGTLFLDEIGELPLESQVKLLRLIQHGEVERVGATSKQTI
                     NVRIIAATHRNLQAMVEDGAFREDLYYRLAVVPLYLPPLRERKEDIPELVEHLFHKAK
                     ERHGMQNVRVAPSLAAHFTGYRWPGNVREMENVIERMLVLSNGEQVTEKDLPEELRLA
                     PMDKPKPLLLELPEEGISLEGIERELLLRALEKFKGNQTQAARYLDISRRTLIYRMEK
                     HGLRQNDGSDE"
     gene            complement(8419..9435)
                     /locus_tag="VFA02_10430"
     CDS             complement(8419..9435)
                     /locus_tag="VFA02_10430"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZU10307.1"
                     /translation="MSLTVRPQQARRAKISALGTYVPPRILTNSDLEKMVETSDQWIV
                     ERTGIRERHLVEKGQATSDMAVEAAKSCLAERGIGPEEVEAIIIATVTPDMMFPATAC
                     LVQNKLGAKGAWGFDLSAACSGFVYALQMGTKLVESGAHSKVLVIGADTMSSIIDYTD
                     RATCILFGDGAGAVLIEPADDSDIGMIDYVHEIDGSGACSLYMPAGGSLNPPSAETVA
                     KKMHYVHQDGQAVYKYAVRKMAEAAEKVLERNGLTGADLGCFIPHQANKRIITSTADR
                     LGMAPERVIINIDRYGNTTAGTIPIAMQTAREEARLKKNDLVLLASVGAGFTVGAALL
                     RWEI"
     gene            complement(9564..9660)
                     /gene="ffs"
                     /locus_tag="VFA02_10435"
     ncRNA           complement(9564..9660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFA02_10435"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            9949..>10158
                     /locus_tag="VFA02_10440"
     CDS             9949..>10158
                     /locus_tag="VFA02_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10308.1"
                     /translation="MKQSILDSAGTGVLDTPKTENPSKPLKVGFVSLGCPKNLVDSEV
                     MMGLLSEAGAQMTTAPDAADVLVVNT"
BASE COUNT         2245 a         2719 c         2958 g         2236 t
ORIGIN      
        1 agtcaactta acagatgatg ctccatttct acatcggcaa catcctgtgg tttgccttca
       61 aaaaatgcgt acagacacgg cacaatcacc agcgtcagca gcgtagatgt gaccagtccg
      121 ccaatcacca cgatggccag agggcgctga atttccgaac ctggcccgat ggcgaaaaga
      181 aacggcacaa gcccaagcgc ggccaccgta gcggtcatca tcacagggcg gaagcgcagg
      241 cgcgtgcctt caatgatcgc gtcttgctgc gacatcccac cttcccgaag tttgcggata
      301 taagaaacaa gcactacacc attcaaaacg gcgatgcccc atagcgcgat gaacccaacc
      361 gatgctggaa cagaaagata ttctcctgag ataaacaacc cgatgacccc gccaatcgaa
      421 gcaaagggca gcaccgtaat aatcagcgca gcaaaacgca gagagttgaa caacaggaac
      481 agcagaaaga agatggcgcc aatcgtgatg ggcacaataa tcatcaggtg gcgcatggcg
      541 cgctgcatgt tctggaattg gccaccccat tggatgaaat agcccgcagg caacggcacc
      601 tgagcattta ccttctgttg cagctcggcc acgtagcctc ccagatcgcg gtcctgcacg
      661 ttgatggcaa cgacgatgcg gcgctttccc atatcgcggt tgatctgcgc agggccttcg
      721 agcacgcgga catcggcgag gtcttcgagc ggcactcgcg gcccgttcgg agaagagatc
      781 atgatctgct tgatgtcatt gatgcttttc cgatactgct gcggcaggcg cacgatggcc
      841 tgaaagcgac gctcgccttc gtagatttct gtagcgcttt ttccggccac ggccatctca
      901 attgtgtcat tcacgtcagc ggcgttgatt ccatagcggg cgatcgctgc acggttgatg
      961 ttgatgacga gatactgctg cccaccgacg cggtctacgc gtgtgtcctg cgtgccgcgg
     1021 atcgaagtgg caacttttgc aacttgctgt gctttttcaa tcagcgtgtc cagatcatcg
     1081 ccgaagattt tgacggcaat atcagcgcgt acgcctgtga ccatctcatc tacacggtcc
     1141 gagatgggct gtgacatcac cagattgatt ccgggaatcg tggccagcgc cttgcgcatt
     1201 ttgtctgcga tgacctcctg cgtcagagtg cgcgggcgct tgtctacagg agtaagagtt
     1261 gcgatgacat cagcttcgtt tggccctgcc gggtcagctg gagattcacc gcgtccgagc
     1321 ctggagacta cgctttctac gccgggaatg tctcgcatga ggcgctgcat ctgcatttcc
     1381 atcttgatgg attcatcaag cgagatattg gggacgcggt cggcattggg gccaagcgtg
     1441 ccttcctgca tctccggaat aaatgatgtt ccgagaaatg gaaacaaaca cagcgaggcc
     1501 acaaacaaga ggaccactgc ggtgacgctc ttctttttgt tctgcatggc ccaggaaaga
     1561 atgcggtcgt agggcctgcg cagaagagaa accagccgcg tgtccttttc ggacccgccc
     1621 ttgaggaaga atgcagagag cacgggcgaa agcgagagcg agagaacaag cgaaagcagc
     1681 agcgcaatcg caatcgtata agccagtggc gcgaacatct tgccttctgt gccttcgagc
     1741 gtcatcagcg ggagaaaaac gagcacgatg atggtgacgc caaagataac gggggtcgct
     1801 acttctgtca cggcatcgag cacaacatgc aggcggctgt cctgactgcc atgactcagc
     1861 ttggcgaaga cgttttccac gacgacaacg gacccgtcca ccatcaggcc gatagcaatg
     1921 gcgagcccac caagcgacat gaggttggcg gaaaggccga catggttcat gatcagaaac
     1981 gtgagcagcg gcgtgagaag caggttcgag cacacgatga ggcttgaacg caggtctccc
     2041 agatacaaga aaagcaccac tacaaccagg acaatgcctt cttccaatac ctctgttacg
     2101 gtgtggatgg ccgcatccac cagccgtgag cggtcataat acggcacgat tttgagaccg
     2161 ccaggaatca tgttcctatt gttgatctca gcgacgcgtt ctttgatttt gctgacaatc
     2221 tcctttgcgt tgccgccagc aatcatcatg acaacgccac ctacggcttc tgtgtacccg
     2281 cctttcacca ttgcgccgta gcggacttct tcgccgatgc ggacgtcagc cacgtcccgc
     2341 acatagacag gagtgcctgc attttccttc aacacgatat tgcggatatc gtccacgccg
     2401 cgaatcaagc cgacgctgcg aatcagatat tgctcggcgt gctggggaag aatgcctccc
     2461 ccagcgtttt cgttgttgcg cttaagggca gcgtaaacat cttcgatggt caggccgtag
     2521 tagcgcagct tttgcggatc tacgagcgtt tcatactgct tgacgaaacc gccggtggaa
     2581 ttgatttcgg ccactcctgg aatcgagcgg agaagagggc gcacgaccca gtcctgaatg
     2641 gtacggcgct ctacgagttc atcatgagta agtgggcgct tgccatcatc cggccgctcc
     2701 agcgtgtact ggtagacttc gccgagcgca ttggagaccg gtccaagcac tggattcacg
     2761 ccttccggca ttcggtcgcg tacttcactc aatcgctcat ttacaagctg gcgttcaaaa
     2821 taaactcctt tcccatcttc aaagacgagc gtgatgagtg agagtcccgg cttgttgaga
     2881 gagcgcatgt cggtcaggcc gggaagcccg gtcattgcaa tctcaatggg aatagtgatg
     2941 aagcgctcaa cttcctcggg ggacttcccc ggggcctcgg ttgcaatctg cacctggaca
     3001 ttggtcacgt cagggaaagc gtctacagaa agatgccgcg cagcgttgat gccaaagcca
     3061 agcaggatga gcgcaacgac aacgacgacg aggcgctgct tcaggatcgc ggagatgagc
     3121 gaccgcatca ctcgcctcct ttgagcaggt cctgtttgcg cttgttgttc agatggaaag
     3181 cgccgtcgag cacaatcttt tcatcaggag aaagtccgcg gagaacgaca cgtctatctg
     3241 agacttccgg gccaagctca acttcgcgca gcagaaattc attttgcgct gtctgaatga
     3301 agacatggtc tttgttttct tcccggacaa cggcggtggc aggaacagtg agcttcttct
     3361 caggctggct ttcaaaggtc atggtggcca gcatttccgg cttaaattcc ccagttggat
     3421 tcagaaggtt catgcgcgcc tgcacggtgc gcgtcgccgg atctaccact gggcttacga
     3481 aggagagctt cccattcacg gttgcattcg gcaatgaggg aatattcgca atgaccgttt
     3541 ttccaatgtg aagcgaagcg gcatgttctt caggcacgtc ggccacaagc cagagactgg
     3601 agaggtcggc aatctggaag gcgagatcgg cgggctgcac gacctgtcca acagtgactt
     3661 tgcgctcaat cactgttccg gagatggaag caatgacggg gtaatcggag ttcagcttgc
     3721 gagttgtttc cagttgacgg atggcgttgt cagacatgcc gagtccgcga agctgcgcgc
     3781 gcaatgccgc cacctcagcg ctggtctgaa gcaattcagc ttcacgcttt tgcagttccg
     3841 ctgttccgat aacatcggac tggaccagtt gttttgcgcg ttgcgcagac tgctcggcca
     3901 gcttttcctg cgagtaggcc ttgatgaaag caaactgcgt atcagaaagg gcattgctgt
     3961 gaagggtggc caggacctgt cctcgagaga cgcgttggcc ttcaaaaaca agcactttcg
     4021 tgatgcgccc ttccacgggg gagccgatgc gggcaacgcg cgaggcgtcg gtttctacgc
     4081 gggccattac tttttcctgg gtggtaattt cctgccattc aggaacgccg gtcttgaggt
     4141 cctcctggag ttcgggcgta atccgcactt taagagggtc tctgagtgcg gagccggcgg
     4201 ttttgctggc ttccgcttcg tgtttgcagc cccatgttcc aagcagcgct gttgtcaaaa
     4261 taattgcgga tattccaatt ctgttcagca tcacaatgtc cccggttaag gcgtagcgcc
     4321 aagttcttgc agatcaatca gtgcagactg gcgcgcaaat tcggcgtcga gcaggtcggt
     4381 acgcacgccc tgcaatacgc gctgcgcatc gagcacttcg agaattccgc gctcgccgaa
     4441 tttataggca gaacgcgcgg cgtctactgc ggcttcggct tcgcgcagcg atccggcctg
     4501 aaggcccagc acctgctgat cagaaatctg atactggtca taggcgcgtt ccagcgctgc
     4561 gagaatttcc agctcacgct gccgcgtaat ggcctgcgaa cgctttactt ctgcctgcgc
     4621 ctcggcgatt ggtccttttc gtctgtccca taacggaagt ggcacattca cgccaaagcg
     4681 gtaaaaggag atgtctggct ggcgctcgta ttcgccatag aaggttggct gcggaatgcg
     4741 ccttgcgcgc tcgttcttga ccagtgtttc tgctcttgcc gtttcggtct tagcttcaga
     4801 gagcgcagga tgcgttgcga aaacgcgctg gcgaagctca tccagcggag ccagatgcgc
     4861 ccgatttcca agagagcctt gcgggtcaaa ctcgtcattt gccgaagcgc cgatcacggc
     4921 cttcagaacg gcctgtgcgt tggagagttc tacttctgcg ctgttgacgg cggcgcgggc
     4981 acgggccagt tccgcctcag cgcgcgtcag ttccaacttg cctttttctc cgacctgcac
     5041 ctccacgctt acgcggcggt gcaggtcatc cacaagggcg agattgtcct ttgcatacgc
     5101 aacctgctgc ttgcggcgca gcacgtcgta aaaagcccgc ttgacatctg cgatgaccgc
     5161 aagacgaatg cctgccagtt gatactcgct gctcagaaac tccaggcggg aggcatgaat
     5221 gcgcgtgcgg cgttcagagg gaatttctat ggtttgtcct gcgctgtaat gctggagaag
     5281 cccaggaact cccggcgtgc gaacgggcag agcactctgg tttccggcga gaaattcaaa
     5341 tgttggattg atatatgctt ttgcgctttg tatgcctgcc tttgcccgct ctacccctgc
     5401 ttctcctgcg cgaagtcgcg gattattttg ttccgcgagc gagagtgcct gctcgagggt
     5461 aaggggcgtt acggcctgcg cgaaaagaag tgacggcgca aggatggcaa caaggagtaa
     5521 aagaatcaac gaacgcacgc cttctgtaaa gcaagcagaa tgccaattct gatttgattg
     5581 attttgctgt attgacactc ctttttcgcc gcatatcgta cagatgaggg gccgggtgtc
     5641 ccatttggga cagaggatgt agtggaaact ggagaaacag atcgccggct cccaacagga
     5701 aaagcaggtc cttcactggc tacgcttcgc tcaggatgac agcttcaagg ggaaaagagt
     5761 ggaacattgc ttgcttgcca gagagcaatg actgtaatcc tggccacgga aaatgggact
     5821 tcccgatatt ctttctttat gggatggagt ccacggctgt ggctggagag gtctgcgccc
     5881 ggagtagcgc tgatcgtgct gctccttctc tgttttgcgg cgctcacgct ggttgtgccg
     5941 ggaaatgatc tgtgggcgca caacattctg catcatctga acttcttgcc tctgatgatt
     6001 gcaggcatgc tgttcgggtg gcgcggcgct gcatggactg cgctactggc cggattcgtg
     6061 aatgctcctt tgattgcgca ccattggcac cgctggcctg tagatgcaaa agaccagatt
     6121 gttgagctgt ctattttcag cgctgcggga ttgattgccg gatatctgtc agaccgcgaa
     6181 cgcgcgcagc ggcgaaatct tgagcagacc aagcgggagc ttgaaaaggt ctatacagag
     6241 ctgcgcgaga atgtcgccca tatgaagaag gcggaacggt tgtctgccgc gggccagctt
     6301 gcagctagtc tggcgcatga aattcgcaat cctttggcaa gcatctcagg cgctgctgga
     6361 atcctgcggc gcggttctgc ttcaccggag tatatgcagg acagcctcga tattattgat
     6421 aaggagtcgc agcggctgaa caaactactc accggttttc taaatttcgc caagccgcgc
     6481 tcgccgcgct tgcagaagac cgacccgaac gcggtgcttg cttcagtggc ctcgcttgct
     6541 tcacatgcgg cgcaggcaag tcatgttgct ctcgtccata aaccactgga aaactgcccg
     6601 gagattgagt gcgatccaga gcagttgaaa caagtgctgc tgaatcttgt aatcaatgct
     6661 gtcgaggctt ctcccgcagg agcaacggta gtgttgcgta ctgtctataa cagtggcccc
     6721 attacgcttg aagttgaaga ccagggaagc ggcatccatg aagacgttgc cgaccagata
     6781 tttgatcctt tttttacgac caagcccaaa ggaacaggac tggggcttgc cgtttcttcc
     6841 atgattcttt cgcagcatgg cggaacgctt tcgttcagca aaaacacggc aggaggcacg
     6901 acgttccgcg ttgaattgcc cctgcatgga gatgcagcgc gtgcaaggta acctcatctt
     6961 gattgtggat gatgacagca gcgtccgccg cgtgatgcag atgcaacttg ctgaggcagg
     7021 atacgacgtg ctgcccgcag ccactgggag cgaggcgcac cgtctgttga tagaacgccg
     7081 gccgaagctc gttattacag acctgagaat gcccgatctg gacggcattg aattgctgcg
     7141 ccgcattgcc gaatcagaaa tccagaccac cgtcatcatg attaccgcct ttggcagcat
     7201 tgaaaccgca gtgcaggcga tgcgactggg cgcgtatgac tacatcacca aacccattga
     7261 ttatgaagcg ctgacgctag cggtccatcg cgccatggag cgccagaacc tgattgatga
     7321 ggtgcgtaat ctgcgttcgg ccctcgacca gcgctatggc tttgaaaaca tcgtcggcca
     7381 ctccaagagt ctgctgcgtg ttctggagat ggcctcgcgc gtagcgcaac acgatacaac
     7441 ggtgctcgtc aacggcgaga caggaaccgg aaaggagctg ttggcgcggg ccgttcatca
     7501 caacagccgc cggcgtaatc agccatttgt gaccatcaac tgcggggcca ttccgaagga
     7561 tctcatcgaa gccgaactct tcggatacgc acgcggtgct tttactggag caagcaacag
     7621 taagccaggg aaggtcgaaa tggctgacgg gggcacgctg tttctggacg aaatcggcga
     7681 gctgccgctt gaatcgcagg tgaaattgct gcgcctgatc cagcacggag aagtggaacg
     7741 tgtaggcgca accagcaagc aaaccatcaa cgtacgcatc attgcggcga cgcaccgcaa
     7801 cttgcaggcc atggtggaag atggagcatt ccgcgaagac ctctattaca ggctcgctgt
     7861 agtgccgctt tatctgccgc cgctgcgtga gcggaaagaa gacattcccg agctggtgga
     7921 gcacctcttt cacaaggcca aagagcggca tggaatgcag aacgtgcgtg ttgctccttc
     7981 tcttgcggca cacttcaccg gataccgttg gccaggaaat gtccgcgaaa tggaaaatgt
     8041 gattgaacgg atgttagtgc tctcgaacgg agagcaggtt acggagaaag accttcctga
     8101 ggaactgcgc ctcgccccaa tggacaagcc gaaaccactg ctgctggaac ttccggagga
     8161 gggcatcagc ctcgagggca ttgaacgcga actgctgctt cgggcgctgg aaaaattcaa
     8221 aggcaatcag acacaggcgg cgcgctatct tgacatcagc cgccgcactc tcatctaccg
     8281 catggagaaa cacggtcttc gtcaaaacga cggaagcgat gagtaagagt tgacatttgt
     8341 tcacgtgggc aattaccaat cgtgccaaaa cataaagtgg tacagcgtac cggtgttgga
     8401 gaaagacaat gattgccttt agatttccca gcgcaacaat gctgctccga ctgtgaatcc
     8461 tgcgccgacg ctggcaagca gcaccaggtc gttctttttg aggcgggctt cttcccgcgc
     8521 ggtctgcatg gcgatgggga tggtgccagc ggtggtattt ccgtagcggt caatgttgat
     8581 gatgacccgt tccggtgcca tgccgaggcg gtctgccgtc gaggtaatga tgcgtttgtt
     8641 ggcctggtgc gggatgaagc atccgaggtc ggccccggtg agtccattgc gttcgaggac
     8701 tttttcggcg gcctcggcca ttttgcgcac ggcgtatttg tagacggcct gtccgtcctg
     8761 atggacgtaa tgcattttct ttgcgacggt ttcggcagaa ggcgggttca ggctccctcc
     8821 ggcgggcatg tagagagagc aggcgccgga gccatcaatt tcatggacat agtcaatcat
     8881 gccgatgtca ctgtcgtcgg caggttcgat gaggacagcg ccggctccgt cgccgaagag
     8941 gatgcaggtg gcgcggtcgg tgtagtcaat gatggaagac atcgtgtccg cgccaatgac
     9001 aaggactttt gagtgcgcgc cggattcgac cagcttggtg cccatttgca acgcatacac
     9061 gaagccggag caggcggcag agaggtcgaa tccccaggcg ccttttgcgc cgagcttgtt
     9121 ctggacgagg caggcggtgg cggggaacat catgtcgggc gtaacggtgg cgatgatgat
     9181 ggcctcgact tcttcggggc cgattccgcg ttcggcgagg cagctcttcg ctgcttcgac
     9241 ggccatgtca ctggtggcct ggcccttttc taccagatgg cgttcgcgaa tgccggtgcg
     9301 ttcaacgatc cactggtcgc tggtttcaac catcttttca agatcagagt tggtgagaat
     9361 gcggggtgga acataggtgc cgagggcgct gattttggcg cgccgcgcct gctgaggacg
     9421 cacggtaagg ctcaaaggct tctctccatt gtgatacgaa gtaaaagcct attttctatg
     9481 aggaggttac gtgatgtcta tgaatggaat ggttcctgtg gggtggaaga gtgtaaaagt
     9541 tctgtcggaa aggaggattt aagtgcaccg gggaacctgc tgcgcacgca ctggcccgtt
     9601 accgttgctc ccttccgggc ctggcggggt tggcgggatt gcgtcgcgca ggacccggca
     9661 cactgttgat gatatcacct tcggtgcggt gaagggcaga gagtagcgat ggcatcctta
     9721 ccgcaacaca accacaacta tgttccggta gaagtctatc tgcggtcttc ctatgaaccc
     9781 gatgccgaat ctgggtgatt gatccggaga cgaagaggta tttcaggttt gaaaatagcg
     9841 agctgcataa agcatccgca tttgtgctgc ctgagcatgg cattcagttc gaaatcgcag
     9901 agattgaaaa actactggat taaaaggccc ttccagtaga atagaaaagt gaagcaatcc
     9961 attctcgaca gtgccggaac cggcgttctc gataccccga aaaccgaaaa tccgtcgaag
    10021 ccgctgaagg tgggctttgt gagcctgggc tgtccgaaaa atctggtgga cagcgaagtg
    10081 atgatgggcc tgcttagtga ggcgggcgcg cagatgacca ctgcaccgga tgctgccgat
    10141 gtcctggtcg tgaacacc
//
LOCUS       DASRBV010000283         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_685796, whole genome shotgun sequence.
ACCESSION   DASRBV010000283 DASRBV010000000
VERSION     DASRBV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685796"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..545)
                     /gene="ssrA"
                     /locus_tag="VFA02_10445"
     tmRNA           complement(194..545)
                     /gene="ssrA"
                     /locus_tag="VFA02_10445"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(629..1759)
                     /locus_tag="VFA02_10450"
     CDS             complement(629..1759)
                     /locus_tag="VFA02_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10309.1"
                     /translation="MPHLISKALLLACWATVAAQSANWQQTFEEGTQAAQQGKFAEAE
                     QDFRAVLNQAPSFAPAYLNLGLALQAEQKPDESITALKHAVALNPKLRGAELFLGIAY
                     YKTGDVSEAIQVLKSASVHFPSDAKVWMWLGVSQLAAEKYDDAAKSLDKAGALDPGDV
                     DILYHRGRAHMLVSKEIFTQMYAKAPKSWRVHQVLAQSFEEQDKPEDAAREYQKAIAL
                     APQEPGLHESLGDMEWMLDHLDQARDAYEAEKQLDPYSASVLYKLGALYIVEEHPEKA
                     IPLLQESLKRNPRQYQANFWLGRAAESSGQTEEAIRQFEIAAKANPDDIDTRRSAYYH
                     LVRLYKKTGRQEEAKAALQEFERLHNLQQTTGAHRMEEKQAP"
     gene            complement(1842..>5306)
                     /locus_tag="VFA02_10455"
     CDS             complement(1842..>5306)
                     /locus_tag="VFA02_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10310.1"
                     /translation="TEVNAGTTYQGKTSSSGYYVFPSVRPGKYILTVAYSGFRDFQKT
                     GISIYIGTRFSQNVTMQAGASTETVQVTANGPGLETSTSDIGTVITSEQVEDLPLAVS
                     GALRSLQTLEFLTPGAVGPGTSGGTTYAKIDGGQTLGSDYLLDGISTQRSENGSGLFD
                     QTTPSVESVQEFRVETTSLPAEFGRTTGGLANFKTRSGTNEYHGVIYDFYKNNVFDAN
                     SWFNNGYLALNAGNPSVQSQFKRPPDTKNDYGITMGGPVRIPHLYDGRDKTFFFFSFE
                     ELRYNNGGTALSTVPTTAERNGDFSATLGGPVQGNPVNPCTGEVLLTGQIFDPNTTQT
                     VGGVQCRTEFKNNQVPTNRSQLAQKILAYIPQPNLPGQINNFAYFTTSTNTQSVYGIR
                     IDQNIGTKHRIFGFWNSRENYTPGNPNFPYPIETGPQVQDFYSKYFRGGWDYIISPSV
                     MNEFVIGSNRINSYNSSRAALSGKDWDQMLGIPNGFGPTFPVFSLGQDGPTIGQANAD
                     DNVDNGIAANDYVNWQKGAHSIRFGGTFRWQQFSYINNGGQAGSFNFFRNQTAGTQNA
                     VGPTGNSVAAFLLGVPGDENRQIFYHHPRWISHYYGAFIQDDWKFRPNLTLNLGFRWD
                     VDTPRWEAEGNSSALNPTAPNPGANNIPGVLEFAGKGAGRNGNMQETWADVYRKDFSP
                     RVGFAWSPGFLQGKSVLRGNYSIYYGPLIYADYGQGLTQGFTANPNYFNSNGFLPWGP
                     LDNGFPTPPSGINLDPTQLNGQAIDYVGKSYGRPAMVQSWSLEVQQEIAKDLVGTIGY
                     LGQHSTRLHALLIYLNDMPEKYLQLGDVLNAPINSPQAIAAGINPPYPNFTTTWGTGV
                     PVEQALRPFPQYGYINGDSYLQNTGQATYNALEAKLQRRFTNGLTLLASYTFSKTITN
                     ADSIQPFWNTLLSQGGTQNPFNLKAEKAVSAQDVPNNFVVSYLYDLPFGKGRKFLAHT
                     HPVVNQIVGGWRIGGIQRYVTGQPMSFYGAIGIPGFDNGIRFNRVPGVSVYSNVARNH
                     KFNPFNYTGPCNGTGYFNCAAFVDPNANRNGGPYHFGTMPRNSADIRTFNYADEDMNI
                     NKTWQFHERFGMDLRLEAFNVFNRHVFAKPDTNPYDPSFGQILNQFNTGQLVLGPRNM
                     QVVLRIHY"
BASE COUNT         1082 a         1328 c         1638 g         1258 t
ORIGIN      
        1 aagacggctc ctgaaaacct tagctgtagg tgaagactca ggttttatgc ggaagttgcg
       61 gccggaaatg agtttttggg accaatagtt ggtccattgt cttcccgatg aaagacaaaa
      121 ttgtaacgaa aacgggccaa caaggccaac ctttgccgtt tgtctaacct gttggcagcg
      181 ggctagttaa atctggtgga ggcggcggga gtcgaacccg cgtccgaaat tgcagtcagc
      241 agagagactc catgctcagt cgcgttccgg aaggtttcgc gttgcgcgct cagaacggac
      301 aagatgcgca cattgctagt ccgtggatct tgtctttacg gcccagaccc agccgcaaag
      361 accagcctgc tgtgcgacgt ccttccgcgg cccgcaggcg aagctgcgga ggacggctac
      421 ttagttaatt aagcagcgta tgccagttga tgatcggcaa ttatcatttt ccaggcgatt
      481 acgggtgccc agcccccggc atgcctcttt gccgcaaaca attccgtcga aaccgtgacg
      541 ccccctttgg gcagcttcgt gggatgtctg aagctatgtg aaagatctgt ttttatttta
      601 cgcctctttt gagggcattg cggatgtgtt atggggcctg cttctcctcc atccggtgcg
      661 cacccgtggt ctgttgcaag ttatgcagtc gctcaaattc ctggagcgct gctttggctt
      721 cctcctgcct tccggtcttt ttatacagcc tgaccaggtg gtagtacgcg gaacgtctgg
      781 tatctatatc gtccggattc gctttggccg caatctcaaa ttggcgaatg gcctcttctg
      841 tctggccgct ggattcagcc gccctcccca gccagaagtt ggcctgatac tggcgggggt
      901 ttcgttttag ggactcctgc aagagaggga tggccttctc cggatgctct tccactatat
      961 acagcgcgcc cagcttgtac aggaccgatg cgctgtaggg gtctaactgt ttttcggctt
     1021 cataggcgtc ccgagcctgg tcgagatggt ccagcatcca ttccatatcg ccgagtgatt
     1081 cgtgcaatcc aggctcctgc ggggccaggg caatggcctt ctgatattca cgtgctgcgt
     1141 cttctggttt gtcctgttct tcaaaggact gcgccaggac ctgatggacc cgccatgact
     1201 tcggtgcctt cgcatacatc tgcgtaaaaa tttctttaga caccagcatg tgggcacgcc
     1261 cgcgatgata caggatgtcc acgtcgccgg gatcgagcgc accggccttg tccagagact
     1321 tcgctgcatc atcgtatttc tcagccgcaa gctgtgagac cccgagccac atccagacct
     1381 ttgcgtcaga ggggaagtgg acagatgcag acttgagcac ttgaatggct tccgaaacat
     1441 ctcctgtttt gtagtacgcg attccgagga acaactcagc accacgcagc ttcggattga
     1501 gagcgactgc atgtttcaat gcggtgatgg attcgtcagg cttctgctcg gcctgaagcg
     1561 caagtccaag attcagataa gctggcgcga atgaaggggc ctggttgagg acagcgcgga
     1621 agtcctgttc ggcctcagca aatttaccct gctgtgctgc ctgtgtgcct tcctcgaagg
     1681 tctgttgcca gtttgcagac tgcgcggcaa cagttgccca acaggcgaga agtagcgctt
     1741 tagagatgag gtgtggcatg ggccttctgg aatggtagtg caaaaagaaa gggctgcgga
     1801 atgatcacaa gctccgcagc cggaaaagac ttcaggagag cctaataatg gatcctcagt
     1861 actacctgca tatttctggg gccaagtaca agctggccgg tattgaactg gttcaggatc
     1921 tggccgaagg acggatcgta tgggttcgtg tccggcttgg caaagacgtg ccggttgaag
     1981 acgttgaatg cttccagacg caggtccatt ccaaaccgtt cgtggaactg ccaggtcttg
     2041 ttgatgttca tgtcctcgtc ggcgtagttg aaggtgcgta tgtctgctga attacgcggc
     2101 atggtgccga agtggtacgg accgccgttg cggttggcgt tcgggtccac gaaagcagcg
     2161 cagttaaaat agcctgtgcc attgcatggg ccggtgtagt tgaaggggtt gaatttgtgg
     2221 tttcgggcca cattactgta aaccgagaca cctgggacgc gattgaagcg gatgccattg
     2281 tcgaagccgg gaatcccaat cgcgccgtag aagctcatgg gctgtccggt tacgtagcgc
     2341 tggatgccgc cgatgcgcca cccgccgacg atctggttta cgaccggatg ggtatgggcc
     2401 aggaatttgc ggcctttccc gaagggcaga tcatagagat agctcacaac aaagttgttg
     2461 ggaacgtcct gcgcgctgac cgccttttca gctttcagat tgaagggatt ttgcgtaccg
     2521 ccctggctca gcagtgtgtt ccagaagggc tggatgctgt cagcgttagt gatggttttt
     2581 gagaaggtat aggaagccaa cagcgtaaga ccgttggtaa agcgccgctg gagtttggct
     2641 tcaagagcgt tgtaggttgc ctgtccggtg ttctggagat agctgtctcc gttgatgtat
     2701 ccgtactgcg gaaatggacg cagcgcttgt tcgactggga cacctgtacc ccatgtagtg
     2761 gtgaagttcg gatagggagg gttgatgccc gcagcgatgg cctgcggcga gttgataggc
     2821 gcgttcagaa catcacccaa ctgaagatac ttctccggca tgtcattcag gtaaatcagc
     2881 agagcatgga gccgcgtgga gtgctggccg agatagccga tggtgccgac caggtctttg
     2941 gcaatttcct gctgaacttc gagcgaccag gactggacca tggcgggccg tccatagctc
     3001 ttgccgacgt aatcaatggc ctgcccattt aactgcgtgg ggtcaagatt gatgccgctg
     3061 ggtggagtag ggaagccatt gtcgagcggg ccccagggca ggaacccgtt cgaattgaag
     3121 taattgggat tggcggtaaa gccctgcgtg agcccttgtc cgtagtcggc atagatgagc
     3181 gggccatagt agatgctgta gtttccgcgc agaacggact tgccctgcaa gaagcccgga
     3241 gaccaggcga agccgacgcg gggagagaag tccttgcgat agacgtcggc ccatgtctcc
     3301 tgcatgtttc cattgcggcc tgctcctttg cctgcaaatt caagcacgcc cgggatgttg
     3361 ttcgcacccg gattgggcgc tgtcgggttc agcgctgaac tgttaccttc agcctcccat
     3421 cgcggagtgt ccacgtccca gcgaaagccg aggttcagag tgaggttggg gcggaacttc
     3481 cagtcatcct gaatgaaggc gccgtagtag tgcgagatcc agcgcggatg gtgatagaag
     3541 atctgccggt tctcatctcc gggtacgccg agcaggaagg ccgcgacgct gttgcctgtt
     3601 gggccgacgg cgttctgcgt tcccgccgtc tggttgcgga agaagttgaa ggaaccagcc
     3661 tggccgccat tgttgatgta agaaaactgc tgccagcgga aggtgccgcc aaagcggatg
     3721 ctgtgcgcgc ccttctgcca gttgacatag tcattggcgg caatgccatt gtctacgttg
     3781 tcatccgcat tggcctggcc gatggtggga ccgtcctgcc cgaggctgaa gacaggaaag
     3841 gtagggccga atccgttggg gatgccaagc atctggtccc agtctttgcc ggagagggcg
     3901 gcacgcgatg agttgtagct attgatacga ttgctgccaa tgacgaactc attcatcacg
     3961 ctgggtgaaa tgatgtagtc ccatccccca cggaagtatt tggagtagaa gtcctgtacc
     4021 tgcggaccag tctcaattgg atagggaaag ttcggattgc cgggagtgta gttttcgcgt
     4081 gagttccaga agccaaagat gcgatgcttt gtgccgatgt tctggtcaat gcgaatcccg
     4141 tagactgact gcgtatttgt gctggtggtg aagtaggcga agttgttaat ttgccctggc
     4201 aggttgggct gcgggatata ggccaggatt ttttgcgcca gctgactgcg gttggttggg
     4261 acctggttat ttttgaattc ggtgcggcat tgcacgccgc caacggtttg cgtggtgttt
     4321 ggatcaaaga tctgtccggt gagaagcact tcgcctgtgc atgggttcac cggatttccc
     4381 tgtactgggc cgccgagcgt cgctgagaag tcgccgttgc gttctgctgt ggttggcacg
     4441 gtgctgagcg ccgtgccgcc attgttgtaa cgcagttcct caaaagaaaa gaagaagaag
     4501 gtcttgtcac ggccatcgta gagatgcgga atgcgcacgg ggccgcccat ggtgatgcca
     4561 tagtcgttct tggtgtctgg cggacgcttg aactgactct gcacactggg gtttccggca
     4621 ttgagggcga gatatccgtt gttgaaccag ctattggcgt cgaagacgtt gtttttgtaa
     4681 aagtcataga tgacgccgtg gtattcatta gtgccgctgc gcgtcttaaa attggcgagg
     4741 ccgcctgtgg tgcggccgaa ctcagcgggc agagacgtgg tttctacgcg gaactcctgg
     4801 acggattcaa cggaaggcgt ggtctggtcg aagagcccgg aaccgttttc tgagcgttgc
     4861 gtactgatac cgtccagcag atagtcactg ccgagggtct ggccgccgtc aatcttggcg
     4921 taagtggtac cgccagaggt gccgggacca actgcgccgg gggtaaggaa ctcgagtgtc
     4981 tgcaaggagc gcaaggcacc ggaaacagcc agcggcaggt cctccacctg ctcagaagtg
     5041 atgaccgtgc cgatgtctga cgtcgaagtt tcaaggccag ggccgttggc agtgacctga
     5101 acagtttcag tggacgcgcc tgcctgcatg gttacattct gtgaaaagcg cgtgccgatg
     5161 tagatggaga tgccagtctt ctgaaaatca cgaaaaccgc tgtaggcgac ggtaaggatg
     5221 tatttgccgg gccggacaga gggaaagaca tagtatccgg atgaggaggt cttcccctga
     5281 taggtggttc cggcattgac ttcagt
//
LOCUS       DASRBV010000284         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_225333, whole genome shotgun sequence.
ACCESSION   DASRBV010000284 DASRBV010000000
VERSION     DASRBV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225333"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1222
                     /locus_tag="VFA02_10460"
     CDS             83..1222
                     /locus_tag="VFA02_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZU10311.1"
                     /translation="MRGWSFPLGRWIGVDLRIHTFFLLLLGLCLLATSLNVPTWRAVF
                     LWGILLLAVIVREIARVITAAYHGVQLRSILLLPIGGLFSYANPDSAERASEGKVQTS
                     IAVAGPLANFAFALVVGALIAGASPQVPIFAKPLVTPLHLMRSVVWLNVILGALNFVP
                     AYPLDAGRILRGGFSRAHGAVKGTRAASGLGQMLGLLAVLGGIALLFIPGSGLGTSLS
                     PWLIMGGFFIFVGAQLEDQGLMFQSVVDTVTMRDVMLTNFATLSPSDTLEDALYKAIH
                     SLQDEFPVVRGANLVGVVSRQGIVEALRSEGNGYVQSIMSRAFQVAQPDDSLGAMIRR
                     MAGGKMSLVPVAESGRIVGIVTLQNLMHSMGMLAEHRRLKRESAS"
     gene            complement(1261..2301)
                     /locus_tag="VFA02_10465"
     CDS             complement(1261..2301)
                     /locus_tag="VFA02_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZU10312.1"
                     /translation="MDLVFIRAVFVLVFAAACYFFRPLGLNPVLSAVLGAAIAALVII
                     FEARARSLSLRRLIGAVAGSVLGIFGAFLFSLVLANSLPQGSMRSVLQIFVLLLMSYV
                     GLVVGASKGDMLNLSALGGLFAGDRQAKRDIKLLDTSAIIDGRIADMAETGFIDGTLV
                     VPEFVLHELQMVADSADSSKRQRGRRGLDVLQRMQGNSLLNVQIIKDDFPQIREVDLK
                     LIELARKIEAKIITNDFNLNKVAQLHRVQVLNINDLANSLKPVVLPGEKMNVLVLKEG
                     KEYDQGVGYLDDGTMVVVDHARRMIGRSVEISVTSVLQTASGKMIFGRVDEAQQKTDV
                     LRVATELREISS"
     gene            2546..>2752
                     /locus_tag="VFA02_10470"
     CDS             2546..>2752
                     /locus_tag="VFA02_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10313.1"
                     /translation="MKAIRTGTVLMAALLSTSLLPAQMEDRPILSVSVPFAFTVENTR
                     MPAGEYIVSVIYRDHLWKLSTVDHK"
BASE COUNT          588 a          777 c          752 g          635 t
ORIGIN      
        1 gaacgatact ttccagcaat gacccgcatc gtcttgagga tataccgcca tatacaattg
       61 aaaaggacga ggacgaacgg caatgcgcgg ctggtcattt ccgctgggtc ggtggatagg
      121 cgtagatcta cgcatccata ccttcttttt attgctgctc ggtctttgcc tgctggccac
      181 cagcctgaac gtgcctacat ggcgcgcggt ctttctctgg ggtatcctgc tgctggccgt
      241 gattgtgcgc gaaatcgcgc gcgtcattac tgctgcttat cacggagtgc agttgcgcag
      301 cattcttctg ctgcccatcg gcgggctctt ttcttatgcc aatcctgaca gcgcagagcg
      361 cgccagcgaa ggcaaggtgc agacttccat agccgtagcg gggccgctgg cgaattttgc
      421 atttgccctg gttgtcggcg cattgattgc cggagcatcg ccgcaggtgc ctatctttgc
      481 caagccgctg gtcacgccat tgcatctgat gcgctcggtc gtatggctga acgtgatcct
      541 gggcgcgctg aactttgtcc ctgcttatcc gctcgatgcc ggacgcatac tgcgcggcgg
      601 cttcagccgc gctcatggcg cagtcaaggg cacacgtgct gcgtccgggc tgggccagat
      661 gctcggattg ctggcagtgc tcggcggaat cgccttgctc ttcatccccg gatcagggtt
      721 aggcaccagc ctgagtccgt ggctcattat gggcggcttc tttatcttcg tgggtgcgca
      781 gcttgaagac caggggctga tgttccagtc cgttgtagac acagtcacca tgcgcgatgt
      841 gatgctgacc aactttgcca cgctttcgcc ctctgacacg ctggaagatg ctctttacaa
      901 ggcaatccac agcctgcagg acgagttccc ggtggtgcgc ggcgcaaacc tcgtcggggt
      961 tgtttcgcgg cagggcattg ttgaagcatt acgcagcgaa ggcaatgggt acgtacagtc
     1021 catcatgtcg cgcgctttcc aggtagcgca gcctgatgat tctcttggtg ccatgattcg
     1081 tcgcatggct ggcggtaaga tgtctctggt accagtcgcg gaaagcggaa ggatcgtagg
     1141 cattgtgacc ctgcaaaacc tgatgcacag catgggcatg ctggccgagc accgcaggct
     1201 gaagcgcgaa agcgcttctt aggtgtgaag tgagttataa attcgcagaa taaatgctga
     1261 ctaactggaa atctctctca gctcagtagc cacacgcaat acatctgtct tctgctgtgc
     1321 ctcatccacg cggccaaaaa tcatcttgcc cgaagcggtt tgcaggacgg acgttacgct
     1381 gatctccacc gagcggccaa tcatacgccg cgcatggtct acgaccacca tggtcccgtc
     1441 atccagatag ccgaccccct ggtcgtattc ctttccttct ttcaggacaa gcacgttcat
     1501 cttctccccg ggcagcacaa caggtttgag tgaattggcg agatcattga tgttcagcac
     1561 ctgcacgcga tgcagttggg ccactttgtt gagattgaag tcgttggtga taattttggc
     1621 ttcaatcttc ctggccagtt cgatgagctt caggtccact tcccggattt gcggaaaatc
     1681 atctttaata atctgcacgt taagtaacga gttgccctgc atacgctgaa gtacgtcgag
     1741 gccgcggcgt cctctctgtc gcttcgacga gtccgccgag tctgccacca tttgtaactc
     1801 gtgcagaaca aattcaggaa cgaccagggt tccatctata aaaccagtct cggccatgtc
     1861 agcgatgcgt ccgtcaataa ttgcgcttgt gtccaacagc tttatatcgc gctttgcctg
     1921 gcggtcgcct gcgaatagcc cgcccagtgc tgaaaggttg agcatgtctc ctttactggc
     1981 acccaccaca aggcccacat acgacatcag cagcaggacg aaaatctgta acacactgcg
     2041 catgcttccc tggggtaggc tattggccag taccaggctg aaaaggaacg ccccgaagat
     2101 gccaagcacg cttcccgcaa ctgcgccaat cagccgtcgc aagctgagag aacgcgcacg
     2161 cgcctcaaag atgatgacca gcgcagcgat ggccgctccc agaacggctg agagtactgg
     2221 attcaggccg agtggccgga agaagtaaca ggcagcagca aaaacaagaa caaaaacagc
     2281 gcgtataaaa acgaggtcca taacgaccgg atcccccgaa tatgatgttc gccgcaggaa
     2341 agggcctctc tcgcacggca cacggagtat acgcacttgg ccatggtttc ggcaaagatt
     2401 cctgcggacc tgcgatggct gtggaggacc caggagagga ttccatacgt tttgccatct
     2461 gctcagggtg gttcgcccaa attgaatcgc gtcacattgc cgctctgcgc aaggatgatt
     2521 tcaaggcaag tccgaggagg tttccatgaa agctattcgt actgggactg ttttgatggc
     2581 agcgctgctt tcaaccagcc tgcttcctgc acagatggag gaccggccca ttctttctgt
     2641 ttcggttcca tttgccttta cggtggaaaa tacgcgaatg ccagccggcg aatacatcgt
     2701 ttcagtcatc taccgcgacc acctttggaa actcagcacg gtagaccaca aa
//
LOCUS       DASRBV010000285         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_225408, whole genome shotgun sequence.
ACCESSION   DASRBV010000285 DASRBV010000000
VERSION     DASRBV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225408"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFA02_10475"
     CDS             complement(<1..1090)
                     /locus_tag="VFA02_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU10314.1"
                     /translation="MAQQKIKRLYLIPILSKALDILELLEEEKRPITLESIYQRSNIS
                     KTSVYRILKTFVHRGYVAQSPDGSYRLVSRPKKMRFGFAGQSAEMPFSEAVTSSLRAA
                     AAASGVELLVRDNRYDADTAIKNAREFVAEGVDLVIEFQIEEHVAPTIAHIISRAGIP
                     LIAIDIPHPHACYFGVDNFQVGYEAGELLAQHAISKWKKKADWVIGLDIAEAGGFVQS
                     RITGAFEGVRSLLPELPQEAFIRLDGRGMRETSARVVADFFKRHQRTDRILIAAATDT
                     SALGALQATLDAQREKNVAIVGQDCIPEVLEEMKKPQSPIIASVSHEANSYGPRLIQL
                     GIAILRGHTVPPYNWVQHKVITAKMVQEQ"
     gene            complement(1145..2569)
                     /locus_tag="VFA02_10480"
     CDS             complement(1145..2569)
                     /locus_tag="VFA02_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZU10315.1"
                     /translation="MPNHLPSIVAIDLGAESCRVSLLRWEQRRPRVDLVYRFSNAPIE
                     RNGRLRWDFKRICAELEKGLQLCAEQTSGPIASIGVDGWAVDYVRLDNDGRSCADPYC
                     YRDMRTEAAEAEVRKHVSAEELFMLGGAQPLRLNTLFQLVADKLAGVPDGTPWVNLPE
                     YVLMHLGGERISEFTNATHTGLVDAKAQTWCEQIFSRCGLDLGAAPKLVSTGTVLGQI
                     HGPLSGMAAFQNTKLIAPCCHDTASAVAGIPLHGDDWAYISSGTWSLVGVLLDRAVLT
                     KEAFQTGFTNLGAAGGQVCFHKNVNGMWLLRQCMAQLCPEGSWTMPELIAAAQHQPTP
                     EALLEVDDPDLLLHGHMASRINAQRKRRGLAPIEESISAMPQFASLIFHSLAARYTEV
                     LGNAQDLSGKRLKRLAIVGGGSQNEFLNRLTSEATGLELVCGVVESSTIGNFAVQLAT
                     LEGASGSRERLAWWADALTGMREC"
     gene            complement(2660..>3545)
                     /locus_tag="VFA02_10485"
     CDS             complement(2660..>3545)
                     /locus_tag="VFA02_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU10316.1"
                     /translation="RIEYWKLEEAKLRRMPPEKELSRQIALVVGGGSGIGREVALLAA
                     ERGAHVVIADRDFAGAERVAEEAKAIAGKEVALAVSVDIRSRDAIRAAMRGTVAAFGG
                     LDILVNTAAIFPSSPDGVITETQWALTLEINVTANFLLVDEIGKLLRDQELAANVVLT
                     SSANAVVPKKGSEAYDVSKAALSHLVRELAVSQAPLVRVNGISPATVVKGSTMFPRDR
                     VIASLKKYEISFDANQSDDELRNLLAKFYAKRTLTHQPIDPRICAEAILFLAGPKAPC
                     TTGHLIPVDGGLADAFLR"
BASE COUNT          783 a         1133 c          899 g          730 t
ORIGIN      
        1 cctgctcctg caccatcttt gccgtaatca ccttgtgctg cacccagttg tagggcggaa
       61 cggtatgccc gcgcagaatg gcaattccta actgaatcaa ccgcggccca tagctgttcg
      121 cttcatgcga gacggacgca ataatcgggc tttgcggctt tttcatctct tccagcactt
      181 caggaatgca gtcctgccca acgatggcaa cgttcttttc ccgctgcgcg tcgagcgtcg
      241 cctgcaatgc gccaagcgcg ctcgtatcag tagcagcagc aatcaggata cggtctgtac
      301 gctggtgacg cttgaaaaag tctgccacta cgcgcgcgct ggtctctctc atgccgcggc
      361 catccaggcg tatgaatgcc tcctgcggaa gctccggcag cagcgagcgg acaccttcaa
      421 acgcgccggt aatgcggctt tgcacaaagc cgcccgcctc agcaatgtca agcccaatca
      481 cccagtccgc ttttttcttc cattttgaaa tcgcgtgctg cgccagcagt tctccggcct
      541 cgtagcccac ctgaaaattg tccacgccaa agtaacaggc atgagggtgt ggaatatcaa
      601 tggcaatcag cggaatgcca gcccgcgaaa taatgtgtgc aatcgttgga gcaacatgct
      661 cttcaatctg gaactcaatc accaggtcta cgccttctgc gacaaactct ctggcatttt
      721 tgatggcggt gtctgcatca tagcggttgt cgcggaccag cagttcaacc cccgaagccg
      781 ccgcagccgc ccgcaaactg ctggtcacag cctcagaaaa cggcatctcc gcgctctgcc
      841 cggcaaaacc gaagcgcatc ttcttggggc gggacaccag gcggtacgag ccatcaggcg
      901 attgcgccac atatccgcga tgcacaaagg tcttcagtat gcggtaaaca ctggtcttgg
      961 aaatatttga gcgctgatag atggactcca gcgtgatagg ccgcttctct tcttcaagaa
     1021 gctcaagaat gtcgagcgct ttggaaagaa tagggatgag ataaagccgc ttgatctttt
     1081 gctgggccaa cttgaaccgc gcctccggca gcgtaagtgt gaaagcgtaa gtatacagcg
     1141 aaaatcagca ttccctcatg cctgtcagcg catccgccca ccaggcgaga cgctcccgcg
     1201 aaccggaggc accttcaagc gtggcaagct ggacggcgaa gttgccaatg gtcgagcttt
     1261 ccaccacccc gcacaccagc tccagtccgg tggcctcgct ggtcagcctg ttgagaaact
     1321 cgttctggct gcccccgccc acaatcgcaa gacgcttcag tcttttgccg ctgaggtcct
     1381 gcgcatttcc gagcacctcc gtatagcgcg ccgccaggct gtgaaaaata agtgaagcaa
     1441 actgcggcat ggccgaaatg ctttcctcaa tcggtgcaag cccgcgccgc tttcgttgcg
     1501 cattgatacg cgaagccata tgaccgtgca gaagaagatc aggatcatct acctccagaa
     1561 gggcctccgg cgtgggttgg tgttgggctg cggcaatcag ctctggcatc gtccagctcc
     1621 cttccggaca aagttgcgcc atacactgcc gcagcagcca catgccattt acgtttttat
     1681 ggaagcagac ctgcccgcct gccgcaccga ggttcgtgaa tccggtctga aatgcctctt
     1741 ttgtcagaac agcgcgatcc agcagcacgc ccaccagcga ccatgttccc gagctgatat
     1801 aggcccaatc gtcaccatgc agcggaatcc cggccacagc cgaagccgtg tcatgacagc
     1861 acggcgcaat gagcttcgtg ttctgaaatg cagccatacc ggaaagcgga ccatggatct
     1921 gccccagcac agttccggtc gaaaccagct tcggagcagc acccagatcc agtccgcaac
     1981 gggaaaatat ctgctcacac cacgtctgcg ccttcgcatc tactagtccg gtgtgggttg
     2041 cattggtaaa ttcagagata cgctctccac ccagatgcat cagcacatat tccggcaggt
     2101 tcacccacgg agtaccatca ggcacgcccg ccagtttgtc cgccacaagc tgaaagagtg
     2161 tgttcaaccg gagtggttgc gcgccaccca gcatgaacaa ttcttccgca gagacatgct
     2221 tccgaacttc ggcctcggca gcctctgtcc gcatatcacg gtaacaatag gggtccgcac
     2281 aggacctgcc gtcgttgtcc agacggacat aatccaccgc ccatccatcc actcctatcg
     2341 aagcaatcgg cccggacgtt tgctccgcgc agagctgcaa acccttctca agctcggcac
     2401 agatgcgctt gaagtcccag cgcagccttc cgttccgctc tatcggagcg ttgctgaagc
     2461 gatagaccag atcaacacgc ggcctgcgct gctcccatcg caacagcgag acacggcagc
     2521 tctccgcgcc cagatcaatc gcaacaatgg aaggaagatg gttcggcatc tttgattttc
     2581 gttctactgg tcttcaaggc cctgggtgcc ccacatctcc acagagatat gggtttgcaa
     2641 agcaaaggca gcttgcccct caccgcagga acgcatccgc cagacctcca tccaccggaa
     2701 tcagatgccc tgtggtacac ggcgcctttg gtccggcaag gaacagaatc gcctcagcac
     2761 agatccgtgg atcaatcggc tggtgcgtca atgtgcgctt cgcataaaac ttggccaaca
     2821 aattgcgaag ttcgtcatcg ctctggtttg catcaaaaga aatctcatac tttttgagcg
     2881 atgcaatcac gcggtcacgc ggaaacatcg tagacccctt cacaactgtc gccggactaa
     2941 tgccattcac ccgcactagc ggcgcctgcg aaacagccag ctcgcgcacc aaatggctca
     3001 gcgcggcctt gctcacgtcg taggcctcgc ttcccttctt gggaacaacg gcgttggccg
     3061 agctggtcag tactacattg gccgccagct cctggtcacg cagcaatttg ccgatttcat
     3121 ctaccagcag aaaattcgcc gtcacattga tttcaagcgt cagcgcccac tgtgtttccg
     3181 taatcacgcc gtccggtgac gagggaaaga tggccgcagt gttcaccaga atatcgagac
     3241 cgccaaaggc agcgaccgtc ccccgcatgg ctgcgcgaat tgcatcgcgg cttcgaatgt
     3301 ccacggaaac cgcaagcgct acctctttgc cggcaatggc ctttgcttct tccgctacgc
     3361 gttcggcccc ggcaaagtcg cggtctgcaa tcacaacgtg cgctccgcgt tccgccgcaa
     3421 gcagcgctac ttctctgcca ataccgcttc cgccgccaac caccagcgca atctgacggc
     3481 tcaattcctt ctccggaggc atacgccgca gcttggcctc ttcgagcttc cagtattcaa
     3541 tgcgg
//
LOCUS       DASRBV010000286        17459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_168428, whole genome shotgun sequence.
ACCESSION   DASRBV010000286 DASRBV010000000
VERSION     DASRBV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168428"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFA02_10490"
     CDS             <1..316
                     /locus_tag="VFA02_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZU10317.1"
                     /translation="GLLHHAESDSSFLGMAVTAGALLIMPLLAKMKREVATQTGNKAL
                     RADAVQSATCAYLAGLTLAGLLIRYIFGIHWIDLLAALLAVPILIVEGKRARSGQTCA
                     CC"
     gene            complement(526..1011)
                     /locus_tag="VFA02_10495"
     CDS             complement(526..1011)
                     /locus_tag="VFA02_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10318.1"
                     /translation="MAFAVVRKLAVLSICVPFALCAQQAKRGRKYTPPPPTCKVTVTV
                     TKGSNGKPVENAGVVFHPMKNGKDDGNMELKTNEEGKAVLDVIPIGDTVRLQVIAKGF
                     QTYGADYVLEGPTEEINIKLNPPGRQYSIYEKHDGSQVGGQDEVQTPKPEEGQSQPQP
                     H"
     gene            complement(1108..3573)
                     /locus_tag="VFA02_10500"
     CDS             complement(1108..3573)
                     /locus_tag="VFA02_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10319.1"
                     /translation="MPELLRRSRFALLSVCLLLAVMPLRAVVVRGRVTDPLGAGIANA
                     DVALVQNGQVIVAGKTQGDGSYYLSTSSSGRFYVLAGGKSFRQLVTESFYGGTLDAVE
                     QNVVLEPEWVRQSVVVTATGTPLPQGQVSASITGLSKTDFLNRADMVDVLRQVAGTNV
                     VQQGQRGGLASIFVRGGNSDANKVLFDGAPIEDIGGRFDLSTVTSTGVEKAEVYRGPN
                     SVLYGSDAAAGVIGFSTPRGSTPFPSLMYEGDAGNFGTYRNEVQLGGTHRAFDYYGGF
                     SDLQTKNSIPQDEYHNIVETANLGWAWSSATTIRGTFRNADIATGLPNAYQFYQIAND
                     GKQSDQNIFASGTIDHTFSDRLHGLARYGLARKREESTQWSPTGIYEPDSGVYLGLPV
                     TIQGANGYSVSGQAILNYAGTYPVTSELVSNRDNLYAQADYQFSPHMLLVAGFRFEDE
                     RGKDYYPGFLNDTLERANYDYMLQIQGDFRNRLFYSLGGGVEKNQLYGTEGTPRVGLS
                     YYVVRPGAGVFHGTKLNFNFAKGVKEPTLTDQFDSLYQVLNTYGGQGTAAQFGLRPIG
                     AERSRSYDGGVEQSLFSERVVFRATYFHNEFGNQIESVPATALYQLLPDLPPDQLQQI
                     IAAYNAVGISPTVNSLAFRAQGVESEVQYGIGKNIFLRGGYTYLDSVVQRSFSSDALQ
                     PTYNTSSSFPNVPIGVYSPLRGARPFRRPPHTGFVSATYTGKHFTVVGNGAFSGRSDD
                     STFLGFSDANFGNSLLLPNRNLDAAYAKLDLGLVYQISHWVGVYTQLDNLLSNQHIGP
                     IGYPSLPFNFRTGLRLALGKEKQ"
     regulatory      complement(3597..3786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3922..4623
                     /locus_tag="VFA02_10505"
     CDS             3922..4623
                     /locus_tag="VFA02_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10320.1"
                     /translation="MNIAVLTQGLYPFEIVLLFGGMVLLLVLLVGLIAGLVRGKVPAL
                     LVPLFIISIVMIAYPSVQSIKYKDLTVTLNNQLQQVLANPTDPNSRKSLAQVADQVAS
                     RPAKDAATIAALASAQYALGNEPSAKRNLNKALQLDPSQPVALTLRNKIVSVEKLQQL
                     SNTVQKDPANAAARQQLQEVTSQVSRTQLANPKALLTLATAQKLTGNEEAAQKTEKKA
                     LAINPNALRMAAPKQ"
     gene            complement(4627..4703)
                     /locus_tag="VFA02_10510"
     tRNA            complement(4627..4703)
                     /locus_tag="VFA02_10510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4667..4669),aa:Arg,seq:cct)
     gene            complement(4777..5778)
                     /locus_tag="VFA02_10515"
     CDS             complement(4777..5778)
                     /locus_tag="VFA02_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZU10321.1"
                     /translation="MNTRVCWLLFVVFAISFCAFSQAPAPAIGPTISVSGIAPGQPVK
                     LVAYGDIRFTETTDTSDTNPRVRQFLVRAVADERPLAIFLTGDTPFIGSNPADWAIYQ
                     QETAAWKAEHLWIFPTTGNHEVKGGHEAGIANYLENFPELKGHLYYSAQIGNVYLISL
                     DTTQPSSPGSPQRTWFQSQLEHLPPNVDFVFILDHMPWMNDVQTQIAAGLPGADEISL
                     RTLLEAEAPKAHAKFIVINGHIHNYERFERNGITYLVSGGGGAKPYPVLIRGSEDLYR
                     GPNEKAPQPHINYHYLVIQIAGKRAEFTMRRVVDPGASALALETRDHFTVVNNEQVK"
     gene            complement(5775..5993)
                     /gene="thiS"
                     /locus_tag="VFA02_10520"
     CDS             complement(5775..5993)
                     /gene="thiS"
                     /locus_tag="VFA02_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZU10322.1"
                     /translation="MQLVINGQQKDFSALAGNPTISDLIAHLGLKGDRVAIEQNGEIV
                     PRSTWETAPLRPGDKLEIVHFVGGGLER"
     gene            complement(6057..6245)
                     /locus_tag="VFA02_10525"
     CDS             complement(6057..6245)
                     /locus_tag="VFA02_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10323.1"
                     /translation="MSTLEHSLGQLDGIASAAEKTEARSSEDDGLGCMRGLAMAMLFN
                     AALVLMGCAAWAVWRWLK"
     gene            6526..6933
                     /locus_tag="VFA02_10530"
     CDS             6526..6933
                     /locus_tag="VFA02_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZU10324.1"
                     /translation="MQDAHYYWNYLPLLLQILAASALAGGMVAASWLIGRRRNTKAKL
                     DAYECGIPVEGDTRGRFSVGFYMVAMLFILFDVEAVFMLPWAVIYRDLPKIAGSRLFG
                     FWEMLVYLGFVAVGIFYVWKKGILDWSADKGDL"
     gene            7023..7517
                     /locus_tag="VFA02_10535"
     CDS             7023..7517
                     /locus_tag="VFA02_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZU10325.1"
                     /translation="MDSAVLGKDAVLAALKENRALSSLMAWNSGAILDATYDRKELTI
                     TIARDQIRGACQTLKNAGYNFFEDLTCVDWYPTEPRFQVTYHILSHALKDRVRLVCPV
                     ESIDPSIDSITSIWPAANYYEREVWDLFGIRFHGHPDLRRIMMPEEWEGHPLRKDYPV
                     EGYR"
     gene            7604..8758
                     /gene="nuoD"
                     /locus_tag="VFA02_10540"
     CDS             7604..8758
                     /gene="nuoD"
                     /locus_tag="VFA02_10540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZU10326.1"
                     /translation="MVLNMGPQHPSTHGVLRLVIEIDGETVLKVVPDIGYLHTGIEKT
                     CEAKFYQQVVPMTDRIDYLCPMTNNLAYCLAVEKLLQLEIPDRAQWMRVLLNELTRLN
                     SHLVWLGTHAMDIGALTVFLYCFREREEILRIFEHVSGQRMMTSYFRVGGLALEPPID
                     FFDRVRRFIKVMPEKIDEYENLLTGNPIFMNRLKGVGYLSAEDAISLGVTGPPLRASG
                     VDWDLRRDMPYSSYEKFQFKVPVSNEGDVWARYLVRIQEMRESVKIVEQALEGMPEGR
                     ITADAPKVVLPDREKMKTQMESLIYHFKIVTEGFTVPAGQVYQAVESPRGEMGYYVVS
                     DGTAKPYRVHMRNPSFATLQALQTMCAGRLIADVVAVIGSIDIVLGEIDR"
     gene            8755..9117
                     /locus_tag="VFA02_10545"
     CDS             8755..9117
                     /locus_tag="VFA02_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10327.1"
                     /translation="MTASEPDQRLFAELWISLVSLVRSYVAGHDLGRPSGHATVDCEE
                     GRLKIRTEEKLMTVAFNCATGKGEWNLYGTSVPERRLADGEFEFSADSTVRLSDRRGA
                     VEMEIAAEAFTARVFDED"
     gene            9269..9778
                     /locus_tag="VFA02_10550"
     CDS             9269..9778
                     /locus_tag="VFA02_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZU10328.1"
                     /translation="MSTPATTIFSPELAARFDRLVTLYPVRRSALIPMLLYAQDEVGY
                     ISDEVVKEVAERIGITELDVRNVLSYYSMLRTKPAGKYNIQVCTNISCMLRGGYEILD
                     HCKKRLGIGHKEVTPDGKFSLEEVECIGACCWAPAIQVNYDFYDELTPEKIDQIIEDY
                     KAGRGKETK"
     gene            9780..11087
                     /gene="nuoF"
                     /locus_tag="VFA02_10555"
     CDS             9780..11087
                     /gene="nuoF"
                     /locus_tag="VFA02_10555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZU10329.1"
                     /translation="MPRLVSHPDEVKIISRRFGLGAENIDKYLELDGYKATQLAIEKG
                     PDWIINEMKASGLRGRGGAGFPTGMKWSFVPKQSERPKYVLVNGDESEPGTCKDHLIF
                     LHDPHAVIEGTIIAGLAIGAKMGFIYLRGEYRYLLKIMEKAVADAYARGFLGKNIFGS
                     GIDFDVVTQTGAGAYEVGEESALMESLEGKRGVPRIKPPFPAVVGLYGGPTVINNAET
                     IATVPHILLMGGEPYAKVGSERNGGTRLFGISGHVERPGVYELPMGYNLKKAIYEVAG
                     GVRGGRGLKAVVPGGSSTPVLKADEIDVGLDFDQMGKAGTMLGSGGIVVIDDQTCIVE
                     FALRTIAFYQHESCGWCIPCREGTDWLKKTIGRVHAGGGVDKDLDNIRYLAENMLGRT
                     FCPLGDAAAMPTIGFIKKFRNEFEDHLNGKPCPYAKHEEPVLV"
     gene            11273..11581
                     /locus_tag="VFA02_10560"
     CDS             11273..11581
                     /locus_tag="VFA02_10560"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZU10330.1"
                     /translation="MTREYLVVYEKGEKNWSAFSPDVPGCGSLGDTVEETRTNMREAL
                     ELYLSETVKAGEPVPEPTATSVDFQEFDPDHQTKQYVVEWLPVTLPEPQSTKTPAQAA
                     "
     gene            11590..13938
                     /gene="nuoG"
                     /locus_tag="VFA02_10565"
     CDS             11590..13938
                     /gene="nuoG"
                     /locus_tag="VFA02_10565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZU10331.1"
                     /translation="MADVTFTVDGKKLTAPAGTLLIDACKAAGIEIPAFCYYPGLSLQ
                     AACRMCLVRIEKMPKLQTACTTTVAEGMVVQTETDEIRQARKGMVELLLGNHPLDCPV
                     CDAGGECELQDMTFKYGAAESKYVEIKQHREEQQWSPVVFFDRPRCILCYRCVRVCGE
                     GMDVWALGIQNRGVGAVIAPNGQDHLECEECGMCIDICPVGALTSGTYRYKTRPWEMT
                     HVATICTHCGDGCKTTLGTRRSNDGMEIVRGDNRDKSGINGDFLCIKGRYAFDFANRT
                     ERLTKPLVRQADGKFREVSWDEAINFAAKRLREVRDAKGGQAIGVIGSNRTTNEENYL
                     LQKFARTVLGTNNIDHHRTADYVSFAAALAGKQDRTASLRDTATAPAILLLGNDPTEQ
                     HPALAWNLRSNVRLNRARVYIANHQEIKLRRQAKQFLQIPHDGYASLVQYLAGDDTAI
                     TANEQTAAFRETIRKEDSLLIIFGSEFRGRDIQAIVDFGLSLPNTKFALLGDYVNSRG
                     AADMGLLPDYLPGYVPVSNAAQFAEEYGATLPSVPGLDLLQMFDAAKSGDLAALYIVG
                     SNPVARYNIDPATLRNTFLVVQEMFLTDTAQLADVVFPAANLYEKAGTVTNTYGDIQL
                     VKKAADKAGTRPDFELIVRLADRMGADPKKLVPFGRGVRADMGQTRGAQAGEADRHSV
                     WLAAHGLEPKLSPFDPFATLDEIQRLVPSYSFSRLELLAGSDQHAKSDLVQIASVPAR
                     RDLVLPAEDNLFTSGTLGRYSDKLKEVLETHTTPKPIEAAAD"
     gene            14033..15085
                     /gene="nuoH"
                     /locus_tag="VFA02_10570"
     CDS             14033..15085
                     /gene="nuoH"
                     /locus_tag="VFA02_10570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZU10332.1"
                     /translation="MEASIWIFLLLSVLKVAVVTVILLTAVAYTVLLERKVVGRIQNR
                     WGPSRVGPFGLLQPLADGIKLFLKEDLMPSNVYKPLYIIAPMIALTCALISIGVVPFG
                     KEIYVKGVDLFQISDINIGLLVILGVTSVGVYGIALSGWSSNSKYSLLGSLRSSAQLV
                     SYELALGLSLVGVVLRAGSFRLRDIVDIQATHGILSWNVFGGLQFVAFLIYLMAAYAE
                     TNRAPFDLPEAESELTAGYHTEYSSMKFAMFFMAEYANMITVSCVATLLFLGGWSSPF
                     GSLLPAWGGPVVQALLPVFWFVVKVFFFLFLYIWVRGTLPRFRYDQLMGFGWKFLLPV
                     AILNIIATSLWLAYRA"
     gene            15153..15650
                     /locus_tag="VFA02_10575"
     CDS             15153..15650
                     /locus_tag="VFA02_10575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZU10333.1"
                     /translation="MHLALFFIFGGLCIAGALNLLLQRHPINSALSLIVVMTSLAVLY
                     LLLGAEFLAAAQVIVYSGAIMVLFTFVIMLLNAGREETTHGSKAAYIVGFPGAAAILG
                     VLAYIFLKQSDNLGTAKLGEYLVTTHDLSRVLFRDLLLPFEVTSVLILIAILGAVALA
                     RKGHE"
     gene            15652..15954
                     /gene="nuoK"
                     /locus_tag="VFA02_10580"
     CDS             15652..15954
                     /gene="nuoK"
                     /locus_tag="VFA02_10580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZU10334.1"
                     /translation="MVPISYYLVLSAILFSIGVGAFLIKRNIITVFMSIELMLNAVNL
                     TFVAFAHQWRHVSGQIFVFFVMVVAAAEAAVGLAIIIAIFRTRNTLNVDQVDLMKL"
     gene            15951..>17459
                     /gene="nuoL"
                     /locus_tag="VFA02_10585"
     CDS             15951..>17459
                     /gene="nuoL"
                     /locus_tag="VFA02_10585"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZU10335.1"
                     /translation="MTSSLHLWLIPIVPFAGFLVNGLLGRRFPKALVTAVAWIATLIP
                     FIQVLTIAARFSSLTLPYTENLGYWIAAGNFHADFAFALDQLTLIMLLVVSGVGFVIH
                     IYSAGYMAEEDGYWRFFAYLNLFMFFMLTLVLAENFLLMFVGWEGVGLASYLLIGYYF
                     DRDSAANAGKKAFVVNRIGDFGFLIALFLLIAHFGTLSFNGIFSQIAQHPEWQGGGLT
                     AIALFLVLGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVTRSHVIFD
                     RSPFALTTIAIIGAATALFAATIGLVQTDIKRVLAYSTVSQLGYMFLACGVTAYSAGI
                     FHLLTHAFFKALLFLAAGSVIHALSGEQDMRVMGGLRKKIPITFWTMTAAVFAIAGFP
                     PFAGFFSKDEILYQAFISPSGAGKILWFVGLLTAGLTSFYMFRLWYLTFFRESRAHEA
                     AAYTSVPGHHAEKEEHKHPVHESPAVMTIPLIILAILSVIGGWVGVPAALGGH"
BASE COUNT         3792 a         4878 c         4781 g         4008 t
ORIGIN      
        1 cggattgctg catcatgcag agtcggattc gagtttcctc ggtatggctg tcacggcggg
       61 agccttactg atcatgccct tgttggccaa gatgaagcgg gaagtcgcca cgcagacagg
      121 aaataaggcg ctgcgcgccg acgcggtgca atcggctaca tgcgcctatc ttgccggact
      181 caccctggct ggactgctta tccggtacat cttcggcatc cattggattg atctactggc
      241 ggcgcttctg gctgtcccca ttttgatcgt ggaaggcaag cgtgcccgca gcggtcagac
      301 ctgtgcctgc tgctagattg tgattgcagc gcgattctca ttcgtctggt tggaatgact
      361 tctacgccgc cactttctgt ccgcgtatct ccccatgagg agacaggctc ggcatttggc
      421 tttgggtttg tgtgccgcct attgcgtcct gacttccgta ctgatttcct tatctgccgt
      481 gcttttcagc tatcgtggca aggtggcccg gcgtgctcgt aacgtttagt gaggttgcgg
      541 ttgagattgt ccctcttcgg gcttcggggt ctggacttca tcctgtccgc ccacttggga
      601 accgtcatgc ttctcataaa tggagtactg gcggcccggt gggttcagct tgatattgat
      661 ctcttccgtc ggcccttcca gaacgtagtc ggccccatac gtctggaagc ccttggcgat
      721 cacctgcaga cgcaccgtgt ctccaatcgg aatcacatcg agcactgcct tgccttcttc
      781 gttggtcttc agttccatgt tgccgtcgtc cttcccgttc ttcatcggat gaaagacaac
      841 gccagcattc tccactggct tgccgttcga gcctttcgtc acagttacag tcaccttgca
      901 ggttggcggc ggaggcgtgt atttacggcc gcgcttcgcc tgctgggcac aaagagcaaa
      961 gggcacacaa atagaaagaa cagcgagttt acggacaact gcaaaagcca tgctactgat
     1021 tgtaaggaat ccaggtcgtt gctgagaagg ggctgagtcg gggtggccca catcctaaaa
     1081 aagatgtggg ccagaccacg tacccggcta ctgtttctcc ttacccaatg caagccgcaa
     1141 cccagtcctg aagttaaacg gcagcgaagg atacccaatt ggcccaatgt gctggttact
     1201 cagcagatta tcaagctgag tataaacacc cacccaatgt gaaatctgat acaccagacc
     1261 aagatcgagc ttcgcatacg ccgcatcaag gttccggtta ggcagcaaca gagaattgcc
     1321 aaaattggca tcgctgaagc ccaggaatgt ggaatcatcg ctgcggccgg aaaacgcgcc
     1381 gttgccaacg accgtaaagt gcttccccgt atacgttgca gaaacaaacc cggtatgcgg
     1441 aggccgccgg aaaggccgcg ctccccgcaa aggtgagtaa actcctatcg gaacattcgg
     1501 gaaagaagag cttgtgttgt acgtaggctg caaggcatcc gaactgaatg aacgctgcac
     1561 tacagaatcg agataggtgt accctcctcg cagaaaaatg ttctttccga tgccgtactg
     1621 tacttcgctc tcgaccccct gcgcgcgaaa tgcaagtgag ttgaccgtgg gcgaaattcc
     1681 aaccgcgttg taagcagcga tgatctgctg taattggtct ggtggaaggt ccggtagaag
     1741 ctgatacagc gcagtcgcgg gaacgctctc gatctggttg ccaaattcgt tatgaaagta
     1801 ggttgcgcgg aagaccacgc gttcgctgaa cagactctgc tcaaccccgc catcgtagct
     1861 gcgcgaacgc tcagcaccaa tcggacgcag accaaattgc gctgccgtac cctgtccgcc
     1921 gtaggtgttc aaaacctggt acagcgaatc gaactggtcg gtcagggtcg gctctttcac
     1981 gcctttggcg aagttgaaat tgagcttcgt cccatgaaaa acaccggccc cgggacgcac
     2041 cacatagtat gacaacccaa cacgaggtgt accttctgta ccgtaaagct ggtttttctc
     2101 tacgccgccg ccgagggagt agaacaggcg gttgcggaag tcgccctgaa tctgaagcat
     2161 gtagtcatag ttggcccgct ccagcgtgtc attcaggaat cccggatagt agtcctttcc
     2221 gcgctcatct tcaaagcgga atccggcaac cagcaacata tgcggcgaaa actggtagtc
     2281 tgcctgcgcg taaagattat cgcggttaga aacaagctct gaggtcaccg gatatgttcc
     2341 ggcatagttc agaatcgcct gcccgctgac ggagtacccg ttcgccccct ggatcgtcac
     2401 aggaaggccc agatatacgc cggaatcagg ctcatagatc ccagttggtg accattgcgt
     2461 gctttcttcg cgcttgcgcg ccagtccata gcgggccagg ccatgcaggc gatcggagaa
     2521 agtgtggtca atcgtcccac ttgcaaagat gttctggtca gattgctttc catcgttcgc
     2581 aatctgataa aactgatatg cgttcggcag tccggtcgcg atgtctgcgt tgcgaaaggt
     2641 cccgcggata gtggttgcag aggaccacgc ccatcccaaa ttcgctgtct ccacgatatt
     2701 gtgatattcg tcttgtggaa tcgagttctt cgtctgcaga tcgctgaacc cgccatagta
     2761 atcgaaagcg cgatgcgtcc cgcccagttg cacttcattg cgatatgtcc cgaaattgcc
     2821 cgcatcgcct tcatacatca gcgacggaaa gggcgtagaa ccccgcggcg tactgaagcc
     2881 aatcacgccg gcggcagcat cggaaccata aagcacactg ttcggcccgc gataaacctc
     2941 ggctttctcc acgcccgtgg aagtgaccgt cgaaagatca aaccgtccgc cgatgtcctc
     3001 gatcggcgcg ccatcaaaca gcactttatt ggcatccgag ttgcctccgc gcacaaagat
     3061 gctggccaga cctccacgct gaccttgttg caccacgttg gtcccggcaa cctggcgcag
     3121 cacgtccacc atatcggcgc gattgagaaa gtcggtcttg ctcaacccgg taatggatgc
     3181 gctcacctgc ccttgcggca acggagttcc ggtcgctgtc actacaacag actgccgcac
     3241 ccactccggc tcaaggacaa cattttgctc caccgcgtcg agagtgcccc catagaaact
     3301 ttcagtgaca agttgccgga aggattttcc tccagcaaga acatagaagc ggccactcga
     3361 tgaagtggaa agataataac ttccatcgcc ctgggtcttg ccggcgacga ttacttgtcc
     3421 gttctgaaca agggcaacat cggcattggc aatgccggcg cccagcggat cagtaacacg
     3481 tccgcgcacc acgacggcac gcagtggcat cacggcaagc aacagacata cagaaaggag
     3541 agcaaagcgg cttcgccgca gaagttctgg catatttcct ccgtccccct cggaactggg
     3601 tcttgtgcgc ctgaggccgg tctcctgact ctgcgcgcct tgtcgtgccg ccttcccgga
     3661 ttcccagtgg ctcatggcac agcagcggtg cgtccttcat accagacgcg ccgctgcgca
     3721 cttacagttg cggggcagtg gcggattttc accgccttcc cgaacacctc atgcgttcat
     3781 gtgaatgtgt gcgaagcgcg acaggggcgc tccctgcctg cattcgttcc gttatgtacc
     3841 ggaaaagatg attattcgtg agaacaaaag cgatgtcaaa aaaccttcga aacggtctat
     3901 catgccctca caccattttc catgaacatc gccgtattga cgcaaggcct ctatccgttt
     3961 gaaattgtgc tgctgttcgg cggcatggtc ctgcttttgg tcctgctggt ggggttgatt
     4021 gctggtcttg tgcgaggcaa agttcccgcg ctgctggtgc ccctgttcat catttccatt
     4081 gtgatgattg catatcccag cgtgcagtcc atcaaatata aagacctgac ggtcacgctg
     4141 aataaccaat tgcagcaagt gttggcaaac cccacagatc cgaattcacg caagagcctt
     4201 gctcaggttg cagatcaggt ggcctcgcgc cccgcaaagg atgccgcaac catcgccgca
     4261 ctcgccagcg cgcaatatgc gctcggcaat gaaccatccg caaagcgaaa cctgaacaag
     4321 gcccttcagt tggacccgtc ccagcccgtc gctcttacac tgaggaacaa aattgtgtca
     4381 gtagagaagc tgcaacagtt gtccaatact gtgcagaagg atccggcaaa tgcagccgcc
     4441 agacagcagc ttcaggaagt aacatctcag gtaagcagga cgcagcttgc gaatcccaaa
     4501 gcactcttga ctctggccac cgcccaaaaa cttactggaa acgaagaagc agcccaaaag
     4561 acggaaaaga aagcgctggc aatcaatcca aatgctttga ggatggctgc gccgaaacag
     4621 taattctggt gaccccggga agattcgaac ttccgacacg cagtttagga aactgctgct
     4681 ctatccacct gagctacggg gccacatcct gttcatctcg gcagttgcgt ggaaactgcc
     4741 cttgttctgt ccgcctgtgc ggatggccac tgaaatttat tttacctgct cattgttcac
     4801 gacagtaaag tggtctcgcg tttccagcgc aagagcagat gcgcccgggt caacgacacg
     4861 acgcatcgtg aactctgcgc gttttcctgc aatctgaatc acgagatagt gatagttgat
     4921 atgaggctgt ggtgctttct cattcggtcc tcgatagaga tcttcactgc cgcgtatcag
     4981 cactggatac ggcttggctc cgccgccgcc tgaaaccaga tacgtaattc cattacgctc
     5041 aaagcgctcg taattatgaa tgtgtccgtt gatgacgata aatttcgcgt gggccttcgg
     5101 cgcttcggcc tcaaggaggg tccggagaga gatttcatct gcgcctggaa gtcctgccgc
     5161 aatttgcgtc tgaacatcgt tcatccaggg catgtggtcg agaataaaaa cgaaatccac
     5221 gttcggcggc aaatgttcca gttgggactg aaaccacgtc cgctgcggcg agccaggact
     5281 gctgggctgc gtcgtatcca gcgagataag ataaacattg ccaatctggg ccgagtagta
     5341 gagatgcccc ttcagttcag ggaaattttc aagataattt gcaatgccgg cctcatgtcc
     5401 gcccttcacc tcatgatttc ccgtcgtggg aaaaatccac agatgctctg ccttccatgc
     5461 tgccgtttcc tgctgataaa ttgcccagtc tgcaggattt gagccgataa aaggagtgtc
     5521 tccggtaaga aaaattgcga gaggtctttc gtcagcaacc gccctgacca gaaattgccg
     5581 gacgcgcgga ttcgtatcgc tggtgtccgt tgtctccgta aagcggatat cgccataggc
     5641 aaccagtttg actggctggc ccggagcaat tccgctgaca gagatggtcg ggccaatcgc
     5701 tggagctggc gcctgactga aagcacagaa tgagatggca aaaacaacga agagaagcca
     5761 gcagaccctg gtattcaccg ctccagcccg ccgccaacga aatgaacaat ttcaagcttg
     5821 tcgccaggac gcagaggagc tgtttcccac gtagagcggg gcacgatctc tccattttgt
     5881 tcgatggcga cgcgatcacc cttcaggcca aggtgcgcaa taagatcgga aatggttgga
     5941 ttcccggcaa gcgcagagaa atctttctgc tggccattga tgacgagttg catactttga
     6001 ggttagcatc tcgcatccga ttcccatcca caacagcatc cggtagcagg agcagactat
     6061 ttgagccacc gccagaccgc ccatgcggca caccccatca atacaagcgc agcattgaag
     6121 agcatcgcca tggccagtcc gcgcatacat cccagcccat catcttcaga agaacgggcc
     6181 tctgtttttt cagcagcaga ggcaatgcca tcaagttgtc caagagaatg ctcaagagtg
     6241 ctcatagtgc tcctcctgga actctatcgg cgtcagcttg cgggtacttc agaaatctct
     6301 gattttgtga ctgcaattgt tccaatttga tacgcaggcc caaaatggac gccgctcaaa
     6361 tttcccctct tttttccaaa aatcttctgg gcgccgctgg accgcgtcat gaaaaatttc
     6421 ctttgtttat gcaggaatta cgctctcttc cttgacggct gtaccgctga gaaactactc
     6481 tcagaagaga ggcttcggcg atcaaccgct taagcattga acagtatgca ggacgcacac
     6541 tactactgga attatctccc gttgctgttg caaattcttg ctgcatcagc gcttgccggt
     6601 ggcatggtgg ccgcttcatg gctcattggg cgccgccgca ataccaaagc aaaacttgat
     6661 gcgtatgaat gcggtattcc ggtggaaggc gacactcgtg gacggttttc tgtcggtttt
     6721 tacatggtag caatgctctt cattctgttc gatgtggaag cagtttttat gttgccgtgg
     6781 gcggtgattt atcgcgacct gccaaaaatt gccggctccc gcctctttgg tttctgggag
     6841 atgctggtct atctcggctt tgttgccgtc ggcatcttct acgtctggaa gaaaggcatt
     6901 ctcgactggt ctgccgataa aggcgattta taagtttcat tcccttgggg ccagtggccc
     6961 ttactgttac caacgtgtac tgctgtaact taaccggctg aattgaaatt caaaaggatg
     7021 caatggactc agctgttcta gggaaagatg ccgtacttgc ggcccttaaa gaaaaccggg
     7081 cgctctcttc tctaatggcc tggaactccg gcgccattct cgacgccact tacgaccgca
     7141 aggaactgac cattaccatt gcccgtgacc agattcgcgg cgcatgtcag acactcaaga
     7201 acgccggata caacttcttt gaagatctga cctgcgtgga ctggtatccg actgaaccgc
     7261 gatttcaggt gacctatcac atcctttctc atgcgttgaa agaccgcgtc cggctcgtct
     7321 gtcctgtcga gagcatagac cccagcattg acagtattac ttccatctgg ccggcggcca
     7381 actattatga gcgtgaggtc tgggacctct ttggcattcg cttccacggt cacccggacc
     7441 tgcgccgcat catgatgcct gaagagtggg aaggccaccc gctgcgcaaa gattatcccg
     7501 tggagggcta ccgctaatgc cgaaggcccc ggaacttgcc gaattcgttg aaacttctac
     7561 cctgctgaag cctcgcccgg aagagggcgc aaaagaccgc acgatggtcc tgaacatggg
     7621 gccgcagcac ccttccacgc acggcgtgct ccgtctggtc atcgagattg acggcgagac
     7681 ggtactcaag gtcgttccgg acatcggcta tctgcacacg ggcattgaga agacctgcga
     7741 agccaagttt tatcagcagg ttgtccccat gaccgaccgc attgactatc tgtgcccgat
     7801 gaccaacaat ctggcctact gtcttgcggt cgagaagctg ctccaacttg aaataccgga
     7861 tcgcgcacag tggatgcgcg ttcttctcaa tgaactgaca cgcctcaact cgcacctggt
     7921 ctggctgggt acgcacgcca tggacatcgg cgcgctcacg gtatttctct actgcttccg
     7981 cgaacgcgaa gagattctcc gcatctttga gcatgtctcc ggccagcgca tgatgacctc
     8041 ctatttccgt gttggaggcc tcgcgctgga gccgccgatt gacttttttg atcgcgtgcg
     8101 caggttcatc aaggtcatgc cggagaagat tgacgaatat gagaaccttc tgaccggcaa
     8161 tccgatcttt atgaaccgcc tcaagggcgt tggctatctg agtgctgaag atgcgatttc
     8221 gctcggcgtg accgggcctc cgctgcgcgc ttcgggtgtg gattgggacc tccggcgcga
     8281 catgccctat tcgagctacg agaagttcca gttcaaggtg cccgtttcaa acgaaggcga
     8341 tgtgtgggcg cggtatctgg tccgtattca ggagatgcgc gaatcggtaa agattgtcga
     8401 gcaggcgctc gaggggatgc cggagggccg catcacagcc gatgctccga aggtcgttct
     8461 gcccgaccgc gaaaagatga agacccagat ggagtcgctc atctaccact tcaaaattgt
     8521 gaccgaaggc tttaccgttc cggctgggca ggtctatcag gcagtcgagt cgcctcgtgg
     8581 cgagatgggc tactacgttg tcagtgacgg cacggccaag ccgtatcgcg tgcatatgcg
     8641 caacccatcg tttgccacgt tgcaggcatt acaaacaatg tgcgccggac ggctgattgc
     8701 cgatgtggtc gctgtgattg gctcgattga tatcgtgctg ggagagattg accggtgacg
     8761 gcgtcagagc ccgatcagcg gctgttcgca gagctttgga tttctctggt ttcgttggtc
     8821 cggtcgtatg tcgccgggca tgatctgggc cgtccgtcgg gccacgcgac agttgactgc
     8881 gaagaaggcc gtctgaagat acgcacggaa gagaagctta tgacggtggc atttaattgt
     8941 gcaacgggta aaggcgagtg gaatctgtat gggacctctg ttccggagcg acgcctggcc
     9001 gatggtgagt ttgagttcag cgcggacagc acagtgagac tcagcgaccg tcgtggcgca
     9061 gtcgagatgg aaatagcagc cgaagccttt acggccaggg tttttgacga agattagaag
     9121 ttttttcaaa acctaatcat tacgtcattc agacgaccag agggaggaag aatctcagcg
     9181 atgttgatgt taatgaatgc gcatcgttaa gaatcttcgc ttcgcagaat gacaacgaat
     9241 ataggggttt ggaacgaagg aattttagat gagcactcct gcaaccacga ttttttcacc
     9301 ggagcttgcc gcgcgcttcg accgcctggt cacgctctat ccggtgcggc gctcggcgct
     9361 gattcccatg ctgctctatg cgcaggacga ggtcggctat atctccgacg aggtcgtgaa
     9421 agaagttgcc gaacgtatcg gcattactga actcgacgtg cgcaacgtgc tcagctacta
     9481 ctccatgctg cgcacgaagc ctgcgggcaa atacaacatc caggtctgca cgaacatctc
     9541 ctgcatgttg cgcggtgggt acgaaattct tgatcactgc aaaaaacgcc tcggcattgg
     9601 ccataaagaa gtcactccgg acgggaaatt ttcgcttgaa gaggtcgagt gtatcggggc
     9661 atgttgctgg gcccctgcca tccaggtgaa ctacgatttt tatgatgaac tgacgcctga
     9721 aaaaatagac cagatcatcg aagactacaa agcaggccgc ggaaaggaga caaagtaaga
     9781 tgccacggct cgtctcccac cccgatgaag taaagatcat ctcccgccgc tttggccttg
     9841 gtgcggaaaa tattgacaaa tacctcgaac tggatggtta taaggccacc cagcttgcca
     9901 ttgagaaggg cccggactgg atcatcaacg agatgaaggc cagcggtctt cgcggacgcg
     9961 gtggcgcagg ctttccgacc gggatgaagt ggtcgtttgt acccaagcag tctgaacggc
    10021 ccaaatacgt ccttgtaaat ggtgacgaga gcgagccggg gacttgcaag gaccacttga
    10081 tctttctgca cgatcctcat gcagtgattg agggtacgat tatcgccgga ctcgccattg
    10141 gcgcgaagat gggatttatt tacctgcgcg gcgagtaccg ttatctgctc aagatcatgg
    10201 aaaaggccgt ggccgacgcc tatgcacgcg gttttctcgg caagaacatc ttcggctctg
    10261 gcattgattt tgatgtggtc acgcagactg gagcgggcgc ctacgaggtc ggtgaagaat
    10321 cggccctgat ggaatcgctc gagggcaagc gtggtgttcc gcgtatcaag ccgcccttcc
    10381 cggctgttgt tggactgtat ggtggcccta cggtcatcaa caatgctgag acgattgcca
    10441 ccgttccgca tattctcctc atgggcgggg agccatatgc gaaggtcggt tcagagcgca
    10501 atggtggaac gcgtctgttc ggtatcagcg gacacgtgga gcgccccggc gtctatgaac
    10561 tgccgatggg ctacaacctg aagaaggcaa tttacgaagt tgctggcggt gtgcgcggtg
    10621 gacgcggtct gaaggcagtt gtgccgggtg ggtcttcgac gccagtactg aaggcggatg
    10681 agattgacgt gggcctcgac tttgatcaga tgggcaaggc gggcacgatg cttggctccg
    10741 gcggaatcgt cgtgattgac gaccagacct gcatcgtcga gtttgcgctg cgcacaatcg
    10801 cgttctatca gcacgagagt tgcggatggt gcattccctg ccgcgaaggg acggactggc
    10861 tgaagaagac gattggccgt gtacacgcag gcggcggagt ggacaaggac ctcgacaaca
    10921 tccgctatct ggcagaaaac atgctgggac ggaccttctg cccgctgggc gatgccgcgg
    10981 ccatgccgac gattggattt atcaagaagt ttcgtaacga atttgaagac catttgaacg
    11041 gaaagccctg cccttacgca aagcatgaag aaccggtgct ggtgtaaagg cagtgaggcc
    11101 aaaagagttg gtgcgtattc tggaaacaga cggttggtac aaaacggggc aaaaggaagc
    11161 cttatgcatt tccggcatct cgcgaaaaaa gcttagtgat cgtaccaatg catactggga
    11221 aagaaatcaa taaaggattg cttcacagta ttctgaaggc ggctggtttg aaatgacacg
    11281 agaatacctg gttgtctacg aaaaaggcga gaaaaactgg agcgcatttt cgcccgacgt
    11341 tccagggtgc gggtcgcttg gagatacggt cgaggaaacg cgcacgaaca tgcgcgaggc
    11401 gttggaactg tatttaagtg aaacggtcaa ggcaggagaa cctgtcccgg agccgaccgc
    11461 aaccagcgtg gattttcagg agtttgatcc agaccatcag acaaaacagt atgtggttga
    11521 gtggctacct gtgacgctac cggaaccgca atcaaccaag acacccgctc aggcggcctg
    11581 aaggaacaaa tggctgacgt aacgtttaca gtagatggca agaaattgac cgcgcccgcc
    11641 gggacgctgc tcattgacgc ctgcaaggca gcgggcatcg aaattcccgc attctgctac
    11701 tatcccggac tctcactcca ggcggcctgc cgcatgtgcc tcgtgcgcat tgagaagatg
    11761 ccgaagctgc agacggcctg caccactacc gttgcggaag gcatggtcgt ccagaccgag
    11821 accgacgaga tcaggcaggc gcgcaaaggc atggtcgagt tgctgctcgg caaccatccg
    11881 ctcgactgcc ccgtctgcga cgcaggcggt gagtgcgagc tgcaggacat gaccttcaaa
    11941 tacggcgctg cggaatcgaa atacgtcgaa atcaagcaac accgtgaaga gcagcagtgg
    12001 tctccggtgg tcttctttga ccgtccgcgc tgcatcctct gttaccgttg cgttcgcgtc
    12061 tgcggcgaag gcatggatgt ctgggcgctc ggcatccaaa accgcggggt tggcgctgtc
    12121 atcgcgccga atggccagga ccatctggaa tgcgaagagt gcggcatgtg cattgacatc
    12181 tgtccggtcg gcgcgctcac ctccggcacc taccgctata agacgcgccc gtgggagatg
    12241 acccacgtcg ccaccatctg tacgcactgc ggcgatggct gcaagaccac tctgggcacg
    12301 cgccgctcca atgatggaat ggaaattgta cgcggagaca accgcgacaa gagcggcatc
    12361 aatggcgatt ttctctgcat caaaggtcgc tacgcgttcg atttcgccaa ccgcaccgag
    12421 cgcctgacca aacctctcgt ccgccaggcc gacggcaagt tcagggaagt ttcgtgggac
    12481 gaagccatta attttgccgc gaaaagactg cgcgaggtcc gtgacgcaaa gggcggacag
    12541 gcaatcgggg tcatcggctc gaaccgcacc acgaacgaag aaaactacct gctgcaaaag
    12601 ttcgcgcgca cagtcctcgg taccaataat attgatcatc accgcacggc ggattatgtc
    12661 tccttcgctg ctgcgcttgc cggcaagcaa gaccgcaccg cctctttgcg cgatacggca
    12721 actgctccgg ccatcctgct attgggcaat gatccgacag agcagcaccc tgcgcttgcc
    12781 tggaacctgc gcagcaatgt ccgtctgaac cgggcgcgcg tttacatcgc caatcatcag
    12841 gaaatcaagc tgcgccgtca ggcgaagcag ttcctgcaaa ttccgcacga cggctacgct
    12901 tcgctggtgc agtatctcgc tggagacgac acagccatta ctgccaatga gcagactgcg
    12961 gccttccgcg aaaccattcg caaggaagac tcgctgctga tcatctttgg ctcagagttc
    13021 cgcggacgtg acatccaggc gattgttgac ttcgggcttt cactgccaaa cacgaagttt
    13081 gcgctactcg gcgattacgt caactcacgc ggcgcggcag atatgggcct tctgcctgac
    13141 tatctgccag gatacgttcc ggtctcgaac gctgcccaat ttgcagaaga atacggcgcg
    13201 accctgccct cggtcccggg cctcgatctg ttgcagatgt ttgacgcggc caagagcggt
    13261 gatcttgcgg cgctctatat tgttggctcg aaccctgtcg cccgctacaa catagatccg
    13321 gcaacactgc ggaacacttt ccttgtagtt caggagatgt tcctgaccga taccgcgcag
    13381 ctggctgatg tggtctttcc tgcagccaat ctttacgaga aggcaggaac ggtcaccaat
    13441 acttatggcg atattcagtt ggtcaagaaa gccgcagaca aggccggaac acgtcctgat
    13501 tttgagctga ttgtccgcct tgccgaccgt atgggcgcag acccgaaaaa gctggtcccg
    13561 tttgggcgcg gcgttcgcgc agacatgggg cagacgcgcg gcgcacaggc aggcgaggcc
    13621 gaccgccaca gcgtatggct ggcggcgcat ggtcttgagc caaagctcag cccgttcgat
    13681 ccatttgcaa cactggatga aatccagcgg ctggtgccaa gttatagctt ctcgcgtctg
    13741 gaactgttgg caggaagcga ccagcacgcg aagtctgatc tggtgcagat tgcgtctgtt
    13801 ccggcgcggc gcgacctggt gctgcctgcg gaagataatc tcttcacgtc aggaacgctg
    13861 ggccgttaca gcgacaagct gaaagaagta ctggaaacac acacaacgcc aaagccaatt
    13921 gaggcagcgg cggattagtg cgggtagaag catcggcttt caggccgatg aataaaggca
    13981 aggcaccaaa ggggcttcag ccccggccaa aaagaatttt ctggaaggcc tgatggaagc
    14041 aagtatctgg atttttctgc tgttaagcgt gctgaaggtc gccgttgtca cggtgattct
    14101 gctgacagcg gtggcatata ccgtattgct ggagcgcaag gtcgtggggc gtatccagaa
    14161 ccgctgggga cccagccgcg ttggcccctt cgggttgttg cagcccctgg ccgacggcat
    14221 caagctcttt ctgaaagaag acctgatgcc ctccaatgtt tataagccgc tctatatcat
    14281 cgcgccaatg atcgcgctca cttgcgcgct tatctcgatt ggcgtggtgc cctttggcaa
    14341 ggaaatctac gtcaagggcg tagacctttt ccagatctct gacatcaata tcgggctgct
    14401 ggtcattctc ggggtcacct cggtgggtgt ttatggcatc gcgctttccg gatggtcgtc
    14461 gaacagtaaa tattcgctgc ttggctctct ccgctcaagc gcccagcttg tgagctatga
    14521 actggccctg gggctttcgc tggtcggggt cgtgctgcgc gccggttcct tccgtctgcg
    14581 cgatatcgtg gacatccagg caacgcacgg cattctttca tggaatgtct ttggcggact
    14641 gcaatttgtt gccttcctta tctatctgat ggcggcctat gcggaaacaa accgcgcgcc
    14701 cttcgatctg cccgaagcag aaagcgaact cactgccgga tatcacactg aatacagttc
    14761 gatgaagttc gccatgttct tcatggccga atacgcgaac atgattaccg taagctgtgt
    14821 ggccactctg ctctttcttg gtggatggtc cagccccttc ggcagtcttc tgcctgcatg
    14881 gggaggtccc gtcgtccagg cgctgcttcc ggtcttctgg ttcgtggtca aggtgttttt
    14941 cttcctgttt ctttatatct gggtgcgcgg aacgcttccg cgcttccgtt atgaccaact
    15001 gatgggcttt ggctggaagt tcctgctgcc cgttgcaatt ctcaatatta ttgcgacaag
    15061 tctgtggctg gcttatcgcg cttagaagtg aataaaacaa ggttgtccca ggtcttgaaa
    15121 agacctgggt tgaacaaact actccaaacg acatgcactt agctttattt ttcatcttcg
    15181 gggggctgtg cattgcagga gcattgaatc ttctgctgca acggcatcct atcaacagcg
    15241 cgctgtcgct catcgttgtc atgacttcgc tcgcagtgtt gtatttgctg cttggggctg
    15301 aattcctcgc cgccgcgcag gtcatcgtct actccggcgc aatcatggtg ctctttacat
    15361 tcgtcatcat gctcttaaac gccggacgcg aagaaacaac ccacggcagc aaggccgcct
    15421 atattgtcgg ctttccagga gcggcggcca ttctcggcgt gctggcttac atcttcctga
    15481 agcaaagcga caatctcgga acagcaaaac tcggcgaata tcttgtcact acgcacgacc
    15541 tcagtcgcgt gctcttccgc gatctgctgc tgccatttga agtcacctcg gtgctgattc
    15601 tgattgcgat tctgggcgct gtggcgctcg caagaaaggg gcacgaatag catggtcccc
    15661 atctcctatt acctggttct gagcgcgatt ctcttttcta ttggcgtcgg agcatttctc
    15721 atcaagcgca acatcatcac cgtttttatg tccattgagc tgatgctgaa tgccgtgaac
    15781 ctcactttcg ttgccttcgc ccaccagtgg cggcatgtca gcggacagat atttgtcttc
    15841 ttcgtcatgg tggtggctgc agccgaggcc gctgtaggac tggcgatcat catcgccatc
    15901 ttccgcaccc gcaacacact caacgttgac caagtggatt tgatgaaact atgaccagtt
    15961 ccctccatct ctggctgatt ccgatcgtcc ctttcgccgg atttctggtg aacggtcttc
    16021 tgggccgccg ctttcctaaa gcgctggtaa cagccgtggc ctggattgcc acgctcattc
    16081 cctttatcca ggtgttgact atcgccgcgc gcttcagctc gctgacgctt ccatataccg
    16141 agaatctcgg ctactggatt gccgctggca atttccatgc tgatttcgcc tttgcgctcg
    16201 accagctcac gctcatcatg ctgcttgtgg tctcgggtgt cggcttcgtc atccatatct
    16261 actccgccgg ttacatggcg gaagaggatg gatactggcg ctttttcgcc tacctgaatc
    16321 tcttcatgtt cttcatgctg acgctggtcc tggcggaaaa cttcctgctc atgtttgtgg
    16381 gatgggaggg cgtgggcctc gcgtcatacc tgctgattgg ctactacttt gaccgcgatt
    16441 ccgcggccaa tgcaggcaag aaagcatttg tcgtcaaccg catcggcgat tttggcttcc
    16501 tgattgcgct cttcctgctc atcgcccact tcggcacctt gagcttcaac ggcatcttct
    16561 cgcagattgc gcagcacccc gaatggcagg gcgggggcct gaccgcgatt gcactcttcc
    16621 tcgtccttgg cgctaccggc aaatcggccc agattcctct ctacgtctgg ctgcccgatg
    16681 cgatggaagg cccgacgcct gtctcagctc tgattcacgc agcgacgatg gtcactgctg
    16741 gcgtttacat ggtcacccgc tcacacgtca tctttgaccg ctcgccgttt gctctcacaa
    16801 cgattgcaat tatcggcgct gcgacggcgc ttttcgccgc aacgatcggc cttgtccaga
    16861 cggacatcaa gcgcgtgctc gcctactcta cggtttcgca gcttggatac atgttcctgg
    16921 cctgcggagt cactgcgtac tccgccggaa tcttccacct gctcacccac gccttcttta
    16981 aagcgctgct cttcctcgcg gctggttctg tcattcatgc cttgagcggc gagcaggaca
    17041 tgcgcgtcat gggcgggctg cgtaaaaaga ttcccattac tttctggacg atgactgccg
    17101 ctgtcttcgc cattgctggt tttccgccct ttgccgggtt cttcagcaag gacgagattc
    17161 tctatcaggc attcatttca ccgagtggcg cagggaaaat tctctggttt gttggccttc
    17221 tcaccgctgg attgacgtct ttctatatgt ttcgcctgtg gtatctgaca ttcttcagag
    17281 aaagccgcgc gcatgaagct gctgcgtaca catctgtacc gggtcaccac gcagaaaaag
    17341 aagagcacaa gcatcctgta catgaaagcc ctgccgtgat gaccattccg ctcatcatcc
    17401 tcgccattct ttccgtcatc ggcggatggg tcggcgttcc ggctgcgctc ggcggacac
//
LOCUS       DASRBV010000287         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_529509, whole genome shotgun sequence.
ACCESSION   DASRBV010000287 DASRBV010000000
VERSION     DASRBV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529509"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VFA02_10590"
     CDS             complement(<1..156)
                     /locus_tag="VFA02_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HZU10336.1"
                     /translation="MASQTGFEKYGRKTRRELFLEQMERVVPWSELEAMVRPHDPKGE
                     NGRPPVGL"
     gene            complement(326..814)
                     /locus_tag="VFA02_10595"
     CDS             complement(326..814)
                     /locus_tag="VFA02_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZU10337.1"
                     /translation="MARSARCARCRRPAQMGRGVPGWQLCPGQKGGEAVGKTKRGKGT
                     KWMVLVDGQGLPLGVRLESASPAEVTLAEATLKEVRVPRAKGRPRQKPKRVIADAGYD
                     SDPLRERLKKRAIELIAPYRKNNKRKRYYDGRKMRRYKRRWIVERTNAWPGSVPPIAG
                     SS"
     gene            complement(832..1062)
                     /locus_tag="VFA02_10600"
                     /pseudo
     CDS             complement(832..1062)
                     /locus_tag="VFA02_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1139..2083)
                     /locus_tag="VFA02_10605"
     CDS             complement(1139..2083)
                     /locus_tag="VFA02_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HZU10338.1"
                     /translation="MKPTASLLFCRCAVAVLLPLIAVPPSFGWGNTGHRLVNRIAVEM
                     LPADVPAFLRSQAAVDEIEYLGPEPDRWRSPAEPELNAMQAPDHYMDLELADMINPLP
                     RRRYEFIAAAYAASITHPAQARDLRPEHIGFQPYITNEVWERLKAAMREYRSLGAKHQ
                     DTKPVEAAVLFYAGWLGHYVGDGAQPLHTTVNYNGWVGKENPNGYTTEHKIHWKFEGI
                     YVDANIREADVKPLVVPLHAVGDEFDDYLAYLRHSSTYVEKVYQLDKTGAFDGAGTQE
                     GKQFTAERIAAGASMLRDLIYAAWVESGKPVPEQRFEN"
     gene            2192..2980
                     /locus_tag="VFA02_10610"
     CDS             2192..2980
                     /locus_tag="VFA02_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZU10339.1"
                     /translation="MLTYLVLPISQRWKKDAYAAAASKQQYSVGMHVRVLLFGVLRDL
                     LPEPIESLEMPAGSTVATVLEHFRARGPQHCRLWESLAVAVNQQYAAPSHPLAEGDEV
                     ALLPPVSGGNLSTHVALVRAPIQADALISRLKQGEDGAVVVFDGIVRNNTRGRRTLYL
                     VYEAYEEMALKQMQSLAQEALTRFPVRDVLLVHRLGKLEVGETSVLIAVASAHRPAAF
                     DACRWLIDTLKKTVPVWKKEHFEDGVVWADGEPFPEELRGPVVS"
     gene            2977..3873
                     /locus_tag="VFA02_10615"
     CDS             2977..3873
                     /locus_tag="VFA02_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU10340.1"
                     /translation="MKSLAVIALLFLGPALRAQNIPPIRVDVRLVNVFVNVTDANGSP
                     VGGLTKDDFTIAEDGHPQKIAVFEKQANMPLSIVLAIDTSGSVNKDLSVEKRAAHNFV
                     HSLLRAQDNLDLIEFSDDVREVVPFTNRLSRIDSGIENLRPGPATALYDAVYLSAQNL
                     ANRPGRKVLVLISDGGNTVKGVDYADALEQAVRSETMVYSIIDVPIEADAGRDTGGEH
                     AMITLSQETGGKYFYADAAHLADAFQKVSDDLRTQYLLAYYPEHRRTDSDFRTITVTV
                     RQPPTAHYTVRHRTGYYASPGQ"
     gene            3890..4210
                     /locus_tag="VFA02_10620"
     CDS             3890..4210
                     /locus_tag="VFA02_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZU10341.1"
                     /translation="MSPSYSRRKQKTPPPGETGQEALYLRFLSEKQVPVAIKLRDGET
                     VTGWIEYFDDRMVRLTREGKPNLFIYKQHIRTITEQTGRHAARNRGARKADELENPAL
                     GEQK"
     gene            4207..5100
                     /locus_tag="VFA02_10625"
     CDS             4207..5100
                     /locus_tag="VFA02_10625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZU10342.1"
                     /translation="MKPLPPGVFLEFAAPAAEIALEAGALIRDFYHRGVATEYKGDVD
                     LVTEADRASEKLIVERLRKQFPTHGIYGEEGTRDNIDREYRWYVDPLDGTTNFAHGFP
                     VFCVSLGLEHRPKGLAPDADGVLTAGVVYDPTRDELFVAEKGKGAYLNGEKIHVSRVN
                     DLAECLMATGFPSRKRHENPNVHFYQQVTLRSHGVRRAGSAALDLAYTACGRFDGYWE
                     FNLNPWDTSAGTLLVLEAGGTVTTFDGGPFRLNSKEVLATNALILNEVIHLFQDMFAG
                     RNLDPVLTPREFAQRRAERGR"
     gene            5272..5532
                     /locus_tag="VFA02_10630"
     CDS             5272..5532
                     /locus_tag="VFA02_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZU10343.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEAPHADQPQLYID
                     PVECIDCGACVPVCPVSAIFAADDLPEKWQDYAQKNASYYGR"
     gene            complement(5831..>6380)
                     /locus_tag="VFA02_10635"
     CDS             complement(5831..>6380)
                     /locus_tag="VFA02_10635"
                     /inference="COORDINATES: protein motif:HMM:NF025432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HZU10344.1"
                     /translation="NLTEKQREEILKTLKARFEKNMGRHKGIVWADVQRRLEDRPEKL
                     WPLFEMERTGGEPDIVGRDKKTGEYIFYDCSAESPKGRRSICYDREALESRKEHKPRD
                     SAVDMAAAMGIEILTEEQYRELQTLGEFDTKTSSWVKTPANIRKLGGALFCDRRFDMV
                     FLYHNGAESYYAARGFRGWLKV"
BASE COUNT         1382 a         2046 c         1617 g         1335 t
ORIGIN      
        1 taatcctacc ggcggacggc cgttctcgcc cttcggatca tgcggccgca ccatcgcctc
       61 cagctcgctc cacggcacca cccgctccat ctgctcgaga aacaactccc gccgcgtctt
      121 ccgtccatac ttctcaaagc cggtctgcga tgccaatgtc tgctgccgca cgtgctctga
      181 agcctccacc tacaaactta tccaatcaga ttgtggatcg cagagttttt cagagatccc
      241 ctagagcgtg tttcataaac accgcctcat tgtgatccag aagcaggcga tgtagaagaa
      301 ggcgcagtaa gtggagagca aatgttcatg acgaaccagc aatcggcgga actgacccgg
      361 gccaggcatt ggttcgttcc acgatccagc gccgcttgta tcgccgcatc tttcgtccgt
      421 cgtagtagcg cttccgcttg ttgtttttcc ggtagggcgc gatcagttcg atagcccgtt
      481 tcttcaaccg ctcgcgcaac ggatcggagt catatccggc atcggcgatt acccgtttgg
      541 gcttttgccg cggccggcct tttgcgcgcg ggacacggac ttctttgagc gtggcttccg
      601 caagcgtaac ttctgccgga gaggcacttt ccagccgaac tcccagcgga agaccttgac
      661 cgtcgaccag taccatccac ttcgttccct tgccgcgctt ggttttaccg actgcttcgc
      721 cccctttttg gccggggcaa agctgccatc caggaacgcc tcgtcccatt tgagcaggcc
      781 ttctgcatcg agcgcaccga gcagagcgcg ccatgcatcc agccagacac cctcgtcctg
      841 ccactgtttc agccgtcgcc agcaggttgc cggcgaggga aactcgtcgg gaagaaatcg
      901 ccaagccgct cccgtttgca gaatccacag aatgccctcg aaacaagccc gattcgatgc
      961 ccatggccgg cctcggttgt ccttcctgcg ccgtccttcc ggcaacaacg gcgcaatcaa
     1021 ctcccactgt tcatccgtca ccagcctctg ccgcttcttc atggcagaag ttcaacaatc
     1081 caaccgcctc aaacggaagt acctgccttg aggccgaacc tatttatgaa acaatgctct
     1141 aattctcaaa acgttgttcc ggaacaggct tcccgctctc aacccaggcg gcatagatca
     1201 aatcgcgcaa catgctcgct ccggcagcga tacgctctgc ggtaaactgc ttgccttcct
     1261 gcgtgcctgc gccatcaaat gcgcccgtct tgtccagttg atagaccttc tcaacatatg
     1321 tggatgagtg ccgcagatag gccaaataat catcgaactc atcacccact gcatgcagag
     1381 gcacaaccag cggctttacg tcggcttccc tgatgtttgc atccacgtaa atgccttcaa
     1441 acttccagtg gattttatgc tcggtggtgt atccattggg attctctttg ccaacccatc
     1501 cgttgtaatt cacagtcgtg tgcaaaggtt gcgcgccatc gcctacatag tggcccagcc
     1561 atccggcata gaaaaggacg gcggcctcga ccggctttgt gtcctgatgc ttcgcgccca
     1621 gactgcgata ctcgcgcatc gccgccttca gccgctccca cacttcattg gtgatgtatg
     1681 gctgaaatcc gatgtgctca ggacgcagat cgcgcgcctg tgccggatgg gtaatggaag
     1741 cagcataggc agcggcaata aattcgtaac gtcgccgcgg cagcggattg atcatatctg
     1801 ccaactccag gtccatgtag tgatcggggg cctgcatagc gttcagctcc ggctccgcag
     1861 gagaacgcca gcggtccggc tcaggcccaa gatactcaat ctcatccaca gccgcctggc
     1921 tgcgcagaaa tgcaggcacg tcggcaggca gcatttccac ggctatgcgg ttgaccaggc
     1981 gatggcccgt attgccccat ccaaaggacg gcggcactgc gataagaggc agcaaaacag
     2041 caacagcaca acggcagaaa agaagagaag cggttggttt catcacagtg agtataaaca
     2101 gacggccacc tgttggagtt gctgacaaca cctcgccccg tcgttagagg catgggattc
     2161 tcaccagtat tgaggaaaat acaaataccg cttgctcaca tacctggtgc tccccatctc
     2221 gcaaagatgg aagaaagacg cttatgccgc ggctgcgtct aaacaacagt attctgtagg
     2281 tatgcacgtg cgcgtcctgt tgtttggtgt cctcagggac ctccttccgg aaccaatcga
     2341 atcgctggaa atgcctgccg ggtccacagt cgccacagtg ctcgagcatt ttcgtgcgcg
     2401 cggcccacag cactgcaggc tctgggaatc gctcgccgtg gccgtaaacc agcagtacgc
     2461 ggcaccgtcg catccgctcg ccgaaggcga tgaagtagcg ctgctccccc cggtcagcgg
     2521 aggtaacctt tccacgcacg ttgctcttgt ccgcgcaccc atccaggccg acgcgctcat
     2581 cagccgcctc aagcagggcg aagacggcgc ggtggtcgtt tttgacggca ttgtgcgcaa
     2641 caatacacgc ggacgccgca ccctctatct cgtctacgag gcctatgagg agatggcgct
     2701 caaacagatg cagtccctcg cgcaggaagc actcacgcgc tttcccgtgc gcgatgtgtt
     2761 gctcgtccac cgcctgggca aactcgaagt aggcgaaacc agcgtgttga ttgccgtggc
     2821 ctccgcgcat cgtcccgcgg cctttgacgc ctgccgctgg ctcattgaca cgctcaagaa
     2881 gaccgtccct gtctggaaga aggagcactt tgaagacggc gtcgtctggg ccgacggcga
     2941 accatttccc gaagaactgc gagggccggt tgtatcatga agtctctggc cgtcatcgcg
     3001 ctgctttttc tggggcctgc tctgcgcgcg cagaacatcc cgcccatccg cgtagacgtg
     3061 cgcctggtca atgtcttcgt caatgtaacg gatgccaacg gctctccggt tggcggactc
     3121 accaaagacg atttcaccat cgccgaggac ggccatccgc aaaaaattgc cgtctttgaa
     3181 aagcaggcca acatgccgct ttccattgtg ctggccattg ataccagcgg cagcgtcaac
     3241 aaagacctgt ccgttgaaaa acgcgccgcg cacaattttg tgcattccct cttacgcgcg
     3301 caggacaatc tcgacctgat tgagttctcc gatgacgtcc gcgaagtcgt ccccttcacc
     3361 aacagactca gcaggattga cagcggcatc gagaacctgc gacccggccc tgcaaccgcg
     3421 ctctatgacg ccgtgtatct ctcggcacag aacctcgcca accggccggg acgcaaagtg
     3481 cttgtgctga tctctgacgg cggcaacacc gtcaaaggcg tagattacgc cgacgcgctc
     3541 gaacaggccg tgcgctcgga aacgatggtc tacagcatca ttgatgttcc cattgaagcc
     3601 gacgccgggc gcgacaccgg cggcgaacac gccatgatca cgctctcgca ggaaacgggc
     3661 ggcaaatatt tctacgccga cgcggcccat ctcgcagacg cctttcaaaa ggtctccgat
     3721 gatctgcgca cgcaatacct gcttgcctat tatcctgaac atcgccgcac agactccgac
     3781 ttccgcacca tcaccgtcac cgtcaggcag ccacccaccg cgcattacac cgtgcgccac
     3841 cgcaccggat actatgccag ccccggccag tagatacaat aatgaaggta tgtccccctc
     3901 ttactcccgc agaaagcaaa agactccacc tcccggagaa accggacagg aagcgctcta
     3961 tctccgcttt ctgagcgaaa aacaggtgcc cgtggccatt aaactgcgcg acggcgagac
     4021 cgtcacagga tggatcgaat acttcgatga ccgcatggta cgcctcacgc gcgaaggcaa
     4081 acccaatctc ttcatctata agcagcatat ccgcaccatt acggaacaga caggccgtca
     4141 tgctgcgcgg aaccgcggcg cgcgaaaggc ggacgaactt gaaaatcctg cattgggaga
     4201 acagaagtga agccattgcc gccgggtgtt tttctggagt ttgccgctcc cgctgctgaa
     4261 atcgcgctcg aagccggagc gctcattcgc gatttttacc atcgcggagt cgccacagaa
     4321 tacaaaggcg atgtggacct tgtcaccgag gccgaccgcg cttcagaaaa actcatcgtt
     4381 gagcgccttc ggaaacagtt ccccactcat ggcatctacg gcgaagaggg cacccgcgac
     4441 aacattgacc gcgaataccg ctggtacgta gaccccctcg acggcaccac caatttcgcg
     4501 catggtttcc cggtcttctg cgtttcgctt ggactcgagc atcgccccaa aggtctcgcc
     4561 ccggacgccg acggcgtcct gacggcgggt gtagtgtacg accccacccg cgatgagttg
     4621 tttgttgcag aaaaaggtaa aggtgcctat ctcaacgggg aaaaaatcca cgtatcgcgc
     4681 gtgaacgatc tggccgaatg cctcatggcg acgggttttc ccagccgcaa acgtcacgaa
     4741 aacccaaatg tccacttcta tcagcaggtc accctgcgct cccatggcgt ccgccgtgcc
     4801 gggtctgccg ctctcgacct cgcctacacc gcctgcggac gctttgatgg atattgggag
     4861 ttcaatctga atccatggga cacttccgcc ggaactctgc tcgtacttga agccggaggc
     4921 accgtcacaa ccttcgatgg cggccccttc cggctgaata gtaaagaagt gctggcaacc
     4981 aacgccctca ttctgaacga agtcatccac ctctttcagg acatgttcgc aggccgcaat
     5041 ctcgatccgg tccttacccc gagagagttt gctcagcgcc gcgctgaacg gggcagatag
     5101 acacagcaca tctcctaaat caaaagccag ctgccccatg tctcgcagag acatgggctt
     5161 ctcatcctcc agcgccattc agtttcagca aaatcggcac gaagcatgtc tgtctgaagg
     5221 ccactccctg ctacaattat ttttaatcac ccacatccgg agaaggcttt catggcttat
     5281 gtaatcgcgg aaccctgtat cggcacgaaa gacacggcct gcgtggatgc gtgcccggta
     5341 gactgcatcc accccaagaa ggacgaggcc cctcacgccg accagcccca gctttatatt
     5401 gatcccgtag agtgcattga ttgcggcgcc tgcgttcccg tgtgccctgt ctcggccatc
     5461 tttgccgccg acgatctgcc tgaaaagtgg caggactacg cccagaagaa cgccagctac
     5521 tacggccgct agcattgggt ccagatgcga ctacagcgcg cagcttcggc tgcgcgtttg
     5581 tgtcttgacc cttttcggcg acatcttcta cagtgccccg gttcattcgg ctcagttcat
     5641 aggaattgtt tatcaacgca tggcctgaag aacagcaatc tccggccagc acccgctctc
     5701 aaaacggtct gacgagcgcg ccgacaacgt caacaccaac gcccccaccc ctcctgggaa
     5761 tttctgttgc acatcagccg ggagaaaaca ccggccccct gaaggtaagg gatgagcgag
     5821 aattcaaatc ttagaccttc aaccacccac ggaatccgcg cgcagcataa taagattccg
     5881 ccccattgtg atagagaaaa accatatcga aacggcggtc gcaaaacagg gcccctccca
     5941 gcttcctgat attcgcaggc gtctttaccc aactcgacgt tttcgtgtcg aactcgccca
     6001 gtgtttgcaa ttcccggtat tgttcttccg tcagaatctc aatgcccata gcggccgcca
     6061 tatctacggc actgtccctg ggtttatgct cttttcgtga ttccagtgcc tcacggtcat
     6121 agcagatgct tctgcgccct ttaggactct ccgccgaaca gtcataaaaa atgtactcgc
     6181 ctgtcttctt atcacggcca acgatgtccg gctcgccgcc cgtcctttcc atctcgaaga
     6241 gcggccacag cttttcagga cggtcctcca gcctcctctg cacatcggcc catacaatgc
     6301 ctttatgccg gcccatgttc ttttcaaaac gggccttgag ggttttaagg atctcttcgc
     6361 gttgcttttc tgtaaggttc
//
LOCUS       DASRBV010000288         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_373511, whole genome shotgun sequence.
ACCESSION   DASRBV010000288 DASRBV010000000
VERSION     DASRBV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373511"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..581
                     /locus_tag="VFA02_10640"
     CDS             84..581
                     /locus_tag="VFA02_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10345.1"
                     /translation="MACAVWVLFIQIRARKLRSADWDALVAGIQPMHMRGLELVALDY
                     LNPERRQLKLEPAEIWGLVGGTEGLRRMEHNADLIIALAAYVRRWNYEESVIVAERIR
                     HDAVQMKRALRGIRKAGLVGKSQVRVPFYIHQAAAAYYLMTKRLLNLYQANQYLLYPR
                     LAEAL"
     gene            complement(663..2450)
                     /gene="recJ"
                     /locus_tag="VFA02_10645"
     CDS             complement(663..2450)
                     /gene="recJ"
                     /locus_tag="VFA02_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZU10346.1"
                     /translation="MSAGAAIESIQSTQPPGLSRTKHKRWIYPETDPAEAAELARSAS
                     IPLIVAELLIARGVRSSQEVAHFLNPQLSDLHDPYAMLGMSAAVTRIRQAIERREPVL
                     IYGDYDVDGTTATILLKTAIEMLGGLARFHIPHRLREGYGMRDDRLADAAAEGIRLVI
                     SVDTGIRAFAAAEAAEVLGLDLIVTDHHLPEKSVPRALAVLNPNQPDCAYPCKHLCGA
                     GVAFKLAQALLEARDAERARTKILPSFLKILAIATVADAVPLLGENRTFVALGLDGLR
                     RPVNAGLRHLMLTANLDPALRRLTPTDIAFRIAPRINAAGRMDIASDVVELFTTREPQ
                     RARQLAEKLERLNTERRAAEAAILAQIIERLKEEAFQQARCIVMDGDGWHRGIIGILA
                     SRIVDQTGKPALVVTRENDEAHGSGRSIPGFHLLNAIESCHDLFTRFGGHAHAVGFSL
                     PSEKIPELRRRLESYALAHLREDDLGTPLTIQALLPLDRITPALFTWLRRMEPCGLGN
                     EEPVFAAQNVRLAAAPRFFRERHARLHLAQGPRAIMFPAIGWHWAERIQQLALQEGSV
                     IHLAYKLRENEHPEFGGLELEIVDIEPAH"
BASE COUNT          534 a          689 c          799 g          549 t
ORIGIN      
        1 caattaagtg caagaagggc cagcagttta aatcatcgtt gagctgactt tctatgtgga
       61 ttcttacctt cttaggtttc gggatggcct gtgcggtctg ggtactcttt atccagatac
      121 gcgcgcgtaa attgcgttct gccgactggg atgcgctggt cgccgggata cagccgatgc
      181 atatgcgcgg gcttgaactg gtcgccctgg actatctcaa ccccgaacgt agacagctta
      241 agctggaacc cgcggagatc tggggactgg taggcggtac cgagggtctc cggcgcatgg
      301 agcacaatgc cgacctgatc atcgccctgg ccgcctatgt gcgccgctgg aattacgagg
      361 aatcggtcat cgtggcggaa cgcatccggc atgatgcagt tcagatgaaa cgcgctttgc
      421 gaggcatccg taaggccggt cttgtgggca agagccaggt ccgggtaccg ttctacatcc
      481 accaggctgc ggcggcttat tacctgatga cgaagcgact gttaaatctc taccaggcca
      541 atcagtatct gctttatccc aggttggctg aagctctgta gagttttttg agagaaagat
      601 tgaggcatgg tcttaaggcc atgcctcttc gtgttttatg gcgttttttg cagtgggcct
      661 gctcagtggg ccggttctat gtccacaatc tccagctcta atccgccaaa ttcagggtgt
      721 tcgttttccc gcagtttata ggcaaggtgg atgacgctgc cttcctgaag ggccaattgt
      781 tgtatgcgtt cggcccagtg ccagccgatg gcgggaaaca taatggcacg cggcccctgt
      841 gccagatgca gacgggcatg gcgctccctg aaaaagcggg gagcggccgc cagacggaca
      901 ttctgcgcgg caaagacagg ttcttcattg cccaggccgc agggttccat gcgccgcagc
      961 caggtgaaga gcgcgggcgt aatgcggtcg agcggcaaaa gggcctgaat ggtcaacggc
     1021 gttccgagat cgtcctcgcg cagatgggcc agagcgtagg actccaggcg gcggcgaagc
     1081 tcggggattt tttctgaggg aagggaaaag ccgactgcat gcgcatgtcc accaaagcgg
     1141 gtgaagaggt catggcagct ttcgatggca ttgagcaggt gaaaaccagg gatggagcgg
     1201 ccagaaccgt gggcttcgtc attctcccga gtaactacaa gcgcgggttt tccggtctgg
     1261 tctacgatgc gtgaggcgag gatgccgatg atgccgcggt gccagccgtc accatccatc
     1321 acaatgcagc gtgcctgctg aaacgcttct tctttcaggc gctctattat ctgtgccagg
     1381 attgcggctt ccgcagcacg acgctcggtg ttgagccgtt ccagcttttc ggccaactgc
     1441 cgcgcgcgct gtggttcgcg tgttgtaaac aactcaacga cgtccgaggc gatgtccatg
     1501 cgtccggcgg cattgatgcg gggcgcgatg cgaaaggcaa tatcggtggg ggtcaatctg
     1561 cgcaatgccg ggtcgaggtt tgcagtcaac atcagatggc gcagtccggc atttaccgga
     1621 cgacgcaaac catccaaccc taaggcaaca aacgtgcggt tttcccccag cagcgggacg
     1681 gcatcagcca cagtggcaat ggccagaatc tttaagaaag aaggaagaat ctttgtccgg
     1741 gcgcgttcgg cgtcgcgtgc ttccagaagg gcctgggcca gtttgaaggc gacacctgct
     1801 ccacaaagat gtttgcaggg gtaagcgcaa tccggctgat tgggattgag cacagccagc
     1861 gccctgggga cgcttttctc cggcaggtga tggtcggtaa caatcagatc aagacccagg
     1921 acttcggccg cctcagcagc cgcgaaggcg cgaatgccgg tatctacgct gatgaccaag
     1981 cggatgccct cggccgctgc atcggccagg cgatcatcgc gcataccata gccttcgcgc
     2041 agacgatgag ggatatgaaa acgcgccagg ccacccagca tttctattgc tgttttcaga
     2101 agaatggttg cggtagtgcc gtctacgtca tagtcgccat agatgaggac cggttcgcgg
     2161 cgctcgattg cctgccggat gcgagttacg gcggcggaca tgccgagcat ggcataggga
     2221 tcatgcaggt cgcttagctg agggttgaga aaatgggcaa cttcctggct ggaccgtaca
     2281 ccgcgggcga tcaataactc ggcgacgatg agtggaatgc tggctgagcg cgccaactcc
     2341 gctgcttccg cagggtcggt ttcagggtaa atccagcgct tgtgctttgt gcgggacaga
     2401 cccggcggct gggtactctg gatggattca atggctgcgc cagcgctcac agctactcgt
     2461 cttcatcctc gttctgttca aaaacgatgt cgccaaagtc ttccaccgat tcaagcaggc
     2521 gctgataacg gtcgtaccag gctgtagaag actcatggag gaaggccact c
//
LOCUS       DASRBV010000289         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_373594, whole genome shotgun sequence.
ACCESSION   DASRBV010000289 DASRBV010000000
VERSION     DASRBV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373594"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFA02_10650"
     CDS             complement(<1..836)
                     /locus_tag="VFA02_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10347.1"
                     /translation="MAAQRKKKKWWLWGGISAAIVIILVAAVAVARGSGAKIEPGRLA
                     KATRSDIAKSVVATGKIQPITKVELKSKASGIVEKLFVDINQHVHKGQVLAQLDQQEI
                     LAQVAAQKAQLSAAQANVVTYQANIAQDRVNAAAPDLPMYKSTYERNVAMQKDGIVSQ
                     QVLDDAQRNYVAAANKRDAAVAQISVDDAKLKQAEAQVQQAQASLEQLNEQLSYTTLV
                     SPMDGVVLSRDVEIGDAVSSILVLGSTATLVMTLGDTTQVYVQGKVDEADIGNVYLGQ
                     PAR"
     gene            1076..2428
                     /locus_tag="VFA02_10655"
     CDS             1076..2428
                     /locus_tag="VFA02_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZU10348.1"
                     /translation="MSGLPLFRFVSIAMLILANGFFVAAEFAIVSLRETRVEQLIAQR
                     RPGARIVRRLQQNLGDFLPAVQFGVTLCSLALGWIGEPVVAALFQEWLTGVPHAAVYA
                     HVIAVPVAFALITYFHVLLGELVPKSLALRKIDQMALGVAGPMDAFIRVTRPAVRLMN
                     RSASLVLKLFHAPMAHESSVHSPEELKLVATAARRSGVLPEFQESLIHRVVEGNEIAA
                     REIMTPRQRIFSLPADMLIEEASARIIEEQHSRIPVYDPARGHDYIIGVIYSKDISRL
                     MHFRAMARTRFADAPFSELRLRQVMREVLVVPETKPVLDLLREFQQRRRHLAIVVDEF
                     GTTVGLVTVEDAIEQLIGEVEDEFDVAARKPLTSASGGVVLDGSVNLRDLETQMNWHL
                     PRDAGVETLAGFLLTRLGKIPKSGESIEYEGRRITVAEMSGHRISKVLVENIAQEPVS
                     "
     gene            2428..3351
                     /locus_tag="VFA02_10660"
     CDS             2428..3351
                     /locus_tag="VFA02_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10349.1"
                     /translation="MRILRSLLFRLAYTVPVLWLVVSLVFLLIHLVPGDPVEQMLGEG
                     ARAQDIAALRHAYGLDVPIQTQYVRYWRSVLHGDLGHSLRLNDSVTHLVLTRYPYTLE
                     LTVAALIIAVLLSVPAGIAAALHRARWQDNVVGVLSLFGLSFPVFASGPILILLVSIK
                     LGWLPVSGAGGWDHLVLPAFTMGAALAAILTRMVRTAMLEELGQDYIRTARAKGLPER
                     TVVYKHALRNAMVPVLTLIGLQFGALLAGAIVTETIFSWPGIGRLTISAISNRDYALV
                     QGCILAVGLTYIFVNLATDLVYMVVNPRIRN"
     gene            complement(3367..3822)
                     /locus_tag="VFA02_10665"
     CDS             complement(3367..3822)
                     /locus_tag="VFA02_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZU10350.1"
                     /translation="MIVGVISDTHGLLRPQAIDALRGVEHILHAGDVGDAAILDQLAQ
                     LAPVTAVRGNIDRSGPCAKLPPAELIELAGVSIYMLHDVHRLDLDPVAAGIQVVISGH
                     SHQPLIETKKGVLYFNPGSAGPRRFSLPVSLGRLEIDTEKIQPKLLTIL"
     gene            3904..4392
                     /locus_tag="VFA02_10670"
     CDS             3904..4392
                     /locus_tag="VFA02_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10351.1"
                     /translation="MTCIRPATPEDIPLIYSLIRGLAEYEREPHSVKITEQELLRDGF
                     GPNPYYECLIAEEEGHPAGFALYFPIYSTWQGPSLHLEDLFVRPEFRGKGIGKALLQA
                     VAAVAVKRGCTRLQWDVLDWNQPAINFYNSIGAVMLHEWRRMRVTGEALEALAAPNEV
                     SA"
     gene            4389..>5688
                     /gene="trmFO"
                     /locus_tag="VFA02_10675"
     CDS             4389..>5688
                     /gene="trmFO"
                     /locus_tag="VFA02_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZU10352.1"
                     /translation="MSRIRVIGGGLAGPEAALIAARMGIEVDLYEMRPVRSTPAHQTA
                     DFGELVCSNSLKSESEHTAPWLLKQEMRRAGSPLLACADASAVPAGHALAVDRVEFSR
                     LINQAVERGPCIKVHREEVTRLDPEDGITIIASGPLTSSALSQEIARLTGSDHLAFYD
                     SISPIVEADSINMERVYFAARWDKGTADYINCPFTKEEYDAFYDALVSAETVQEKEWE
                     KLCYFEGCLPIEELARRGRDTLRFGPMKPVGLRDPRTGKTPYAVVQLRCENLRADSYN
                     LVGFQNHLKYGEQARVLRMIPGLENAKFLRYGQIHRNTYINAPRVLTKTLNMREHPKI
                     YFAGQISGVEGYTESIATGLLAGMYAASAALGIPVAPVPRETALGSLVNYITHADAKH
                     FQPANITFDLLVPLEDELRRKVRDKKERHRIQCERALQAFD"
BASE COUNT         1138 a         1570 c         1644 g         1336 t
ORIGIN      
        1 cgcgcaggtt ggccaagata aacattgccg atgtcggcct catctacttt gccctgaacg
       61 tagacctgcg tagtgtcgcc aagtgtcatg accaatgtcg ccgtggaacc gagcacaaga
      121 atagagctga ccgcatcgcc aatttcaaca tcgcgcgaga gcaccacgcc atccatagga
      181 gaaaccaggg tggtatagct gagttgttcg ttcaactgtt ccagactggc ctgggcctgc
      241 tgcacttgtg cttcggcctg cttcagtttg gcgtcatcca cactgatttg cgctacagcg
      301 gcatcgcgct tattggcagc ggccacatag ttgcgttggg cgtcatcaag tacctgttgc
      361 gaaacaatgc cgtccttctg catggccacg ttgcgctcat aggtggactt gtacatgggg
      421 agatcagggg cggcagcgtt gacccggtct tgcgcgatat tggcctgata ggtaacaaca
      481 ttggcctgcg cggcggaaag ttgcgccttc tgcgccgcaa cttgtgcaag gatttcctgc
      541 tggtcaagct gagcaagtac ttgtccttta tggacgtgct gattgatatc tacaaagagt
      601 ttttcaacaa taccgctggc ctttgattta agttcaactt tcgtgatggg ttggattttt
      661 cctgtagcga cgacgctttt cgcaatgtcg ctgcgtgtgg ctttggcaag gcggcccggt
      721 tcgatttttg ctccactgcc acgcgccact gcgacggccg caaccaaaat aatcactatg
      781 gctgcggaga ttccacccca gagccaccac tttttcttct tccgttgcgc cgccatagaa
      841 cacactcccg ctcattggac tcagtggcgg cctgtttcgt actcccgccg aatggtgtat
      901 acgcatcggg aagaggagtg gttcccgtga cggggcgcag ggcacaggga ccccggacgc
      961 atgtgccgga aagacctgga tcaagccagc ttcttcagcg tcctctgtcc ctgatgtatt
     1021 ccctgggtct ttgaaacctg tgactgtgct ctgaaattga tagactcaga aaaaaatgtc
     1081 tggtcttccg cttttccggt ttgtttccat tgccatgttg attctggcga acgggttctt
     1141 cgtggctgcc gaatttgcca tcgtcagctt gcgcgagacc cgggtggagc aacttattgc
     1201 acagcggcgt ccgggcgcgc gcattgtccg gcggttgcag cagaatctgg gagacttttt
     1261 acctgcggtc caatttggcg ttacactttg cagcctggca ctgggctgga ttggcgagcc
     1321 ggtagtcgcc gcgctcttcc aggaatggct gactggggtc ccccatgcgg ctgtgtacgc
     1381 acatgtgatt gccgtcccgg tggcctttgc cctgattacc tatttccatg ttctcctggg
     1441 cgagcttgtt ccaaaatcat tagcgcttcg caagatagac cagatggcgc tcggcgtggc
     1501 tggcccaatg gacgccttta tccgtgtaac gcggcctgcc gtccgcctca tgaaccgctc
     1561 agcatcgctc gtgctcaagc ttttccatgc gcccatggca catgagtcgt ccgtgcattc
     1621 gcccgaagag ttaaagctgg tggccactgc ggcgcggcgg tcaggcgttc tgcctgagtt
     1681 tcaggaatcg ctgatccatc gtgtggtgga aggaaacgag attgccgccc gtgagatcat
     1741 gacgccccgg cagcgcattt tttcgcttcc ggcggacatg ctcatcgaag aggccagtgc
     1801 gcggatcatc gaagagcagc actcgcgtat tccggtttac gatccggcgc gcgggcacga
     1861 ctacattatt ggagtcatct attccaaaga catttcgcgg ctgatgcact ttcgcgcgat
     1921 ggcccgcacg cgctttgctg atgcaccttt ttctgaattg cgcctgcgcc aggtgatgcg
     1981 cgaagtgctg gtcgtgccgg aaacaaagcc cgtgctcgac ctgctgcgcg agttccagca
     2041 gcgtcgccgc catctcgcca tcgtggtgga tgagtttggg acaaccgtcg gtctcgtgac
     2101 ggtagaagat gccatcgagc aattgatcgg cgaagtggaa gatgaattcg acgtggctgc
     2161 gcgcaaaccg cttacctcag caagtggagg ggtggtgctc gatggaagcg tcaatctgcg
     2221 cgacctggaa acacagatga actggcacct tccgcgcgat gccggagtag aaacgctggc
     2281 cggatttttg ctcacccgcc tcggcaagat ccccaaaagc ggcgaatcaa ttgagtatga
     2341 agggcgcaga attacggtcg ctgagatgag cggacaccgc atcagcaaag tgctggtgga
     2401 aaacattgcg caggagcccg tctcctgatg cggattttgc gctcgcttct ctttcgtctc
     2461 gcgtataccg tccctgtcct ctggctggtg gtctcgcttg tgttcctgtt gatccacctt
     2521 gttcccggcg atccggtcga gcagatgctg ggcgaaggtg cgcgtgcaca ggacattgcc
     2581 gcattacgtc acgcctatgg gcttgacgtc cccattcaga cgcagtatgt ccggtattgg
     2641 cgcagcgttc tgcatggtga cctgggccat tctctgcggc tcaatgattc ggtcacgcat
     2701 ctggtattga cccgctatcc ctatacgctg gagctgacgg ttgccgcgct cataattgcg
     2761 gtccttcttt cggttcctgc gggcattgcc gccgcattgc accgcgcgcg ctggcaggac
     2821 aatgttgtag gcgtgctcag cctgttcggg ctttcatttc cagtcttcgc ctccgggcca
     2881 atcctgattc tgctggtttc gatcaagctt ggatggctgc cggtaagcgg tgctggcgga
     2941 tgggaccatc ttgtgctgcc ggccttcacc atgggcgctg ctcttgctgc aattctcacc
     3001 cgcatggtgc gcacggcgat gctcgaagaa ctggggcagg actatatccg aactgcacgc
     3061 gccaaaggtc tgcctgagcg caccgttgtc tacaaacacg cattgcgcaa tgccatggtt
     3121 cctgtgctaa cgctgattgg gttacagttt ggcgcgctcc ttgccggtgc cattgtgaca
     3181 gagacaatct tcagctggcc gggaattggc cgcctgacca tctcagccat ttccaaccgc
     3241 gattacgccc tcgtgcaggg ttgcatcctg gcagtgggac tgacttatat ctttgtgaat
     3301 cttgcaacgg atttggtgta tatggtggtg aatccgcgaa ttcgcaattg atttacccca
     3361 cggcctttaa agaatcgtga gtagctttgg ttggatcttt tcggtgtcaa tttccaatcg
     3421 tcccagcgac accggcagcg aaaatcttct cggcccggcg cttcccggat tgaaataaag
     3481 cacgcctttc tttgtctcaa tcagcggctg gtgtgaatgt ccgctgatga cgacctggat
     3541 gcccgcggca actggatcaa gatcgagtct atgaacatcg tggagcatat aaatactgac
     3601 cccggcaagt tctatgagtt cggcaggagg aagctttgcg caagggccgc tgcggtctat
     3661 attgccgcga accgctgtaa ccggggcgag ctgcgcaagc tggtcgagaa tcgcagcgtc
     3721 gcctacgtct ccggcatgga ggatgtgctc aacgccgcgc agggcgtcaa tggcttgtgg
     3781 gcgaagcagg ccgtgcgtat ctgaaataac gccgactatc atagaaggac ctttattttg
     3841 accagcttac aaaaagaaca ggaagcgggg ccagaacact ccaataaaat tgaagaaaga
     3901 cccatgacct gtattcgccc agctacacct gaagatattc cgctgatcta ttcactgatt
     3961 cgcgggctcg ccgaatatga gcgcgagcca cattccgtaa agattaccga gcaggagttg
     4021 ctgcgcgacg gcttcgggcc gaatccgtac tatgaatgcc tgattgcaga ggaagaaggc
     4081 catcctgcgg gatttgccct ttattttccg atttactcaa catggcaggg gccaagtctg
     4141 catctcgaag atctctttgt gcgcccggaa tttcgcggga agggaattgg caaggcactc
     4201 ctgcaggccg tggctgctgt cgcggtgaag cgtggatgca cgcgcctgca atgggacgtg
     4261 ctcgactgga accagcctgc tatcaacttc tacaactcga taggtgcggt catgctgcac
     4321 gaatggcgtc ggatgcgcgt gacaggcgag gccctggaag cgcttgctgc tccgaatgag
     4381 gtttcggcat gagccgcatt cgtgtcatcg gcggaggact ggcaggcccg gaggccgcgc
     4441 tgattgcggc gcgcatgggc atagaggtgg acctttatga gatgcgtcct gtacgttcga
     4501 ctccagcgca ccagacggcg gactttggcg agcttgtctg ctcgaattca ttgaaatcag
     4561 agagcgagca caccgcgccg tggctgctga agcaggagat gcgccgggcc gggtctccgc
     4621 tgcttgcctg tgcagacgcc agcgccgttc ctgccggaca tgcgctggct gtggaccgag
     4681 tcgagttctc gcggctcatc aaccaagcgg ttgaacgcgg accgtgcatc aaggtacacc
     4741 gggaagaagt gaccaggctt gatccggaag acggcatcac catcattgct tctggtccac
     4801 tgacttcatc agcgctctcg caagagattg cccgccttac cggaagtgat cacctcgctt
     4861 tttacgactc catcagcccg attgttgagg ccgattccat caacatggag cgcgtttatt
     4921 ttgccgcgcg ttgggacaag ggcacagccg attacatcaa ctgcccattc acaaaagaag
     4981 aatacgacgc tttctacgat gcgttggtct cagccgaaac cgtgcaggaa aaggagtggg
     5041 agaagctctg ttatttcgag ggctgtctgc cgattgaaga actggcgcgg cgcgggcgcg
     5101 ataccctgcg ctttggccca atgaagcctg tagggctgcg cgatccgcgc accggaaaga
     5161 caccctatgc cgtggtgcaa ctgcgctgcg agaatcttcg ggcggattcg tacaatctgg
     5221 tcggcttcca gaaccacctg aaatacggcg agcaggcgcg cgtgctgcgt atgattcccg
     5281 ggctggagaa cgcgaaattt ctccgctatg ggcagatcca tcgcaataca tatatcaatg
     5341 ctccgcgcgt gctgacaaag acgctgaaca tgcgcgaaca tccaaagatt tattttgcag
     5401 gacagatttc cggggttgaa ggctataccg aatctattgc cacaggcttg ctcgccggaa
     5461 tgtacgctgc ttctgctgcg ctgggaattc ctgtagctcc ggttccacgt gagacggcgc
     5521 ttggttcgct ggtgaattat attactcacg ctgatgcaaa acacttccag ccggcaaata
     5581 ttacctttga tttactcgtt cctctggaag atgagctgcg aagaaaggtt cgcgacaaga
     5641 aagaacggca ccggatccag tgcgaacgcg cgttgcaggc gtttgatg
//
LOCUS       DASRBV010000290         8179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_272824, whole genome shotgun sequence.
ACCESSION   DASRBV010000290 DASRBV010000000
VERSION     DASRBV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272824"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /gene="rpsH"
                     /locus_tag="VFA02_10680"
     CDS             complement(<1..248)
                     /gene="rpsH"
                     /locus_tag="VFA02_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZU10353.1"
                     /translation="MSFTDPVADFLTRLRNAIRARHQKLDVPASKLKTEIARILKEEG
                     YIANYKTSDENGRAVLRVYLKYGANNEAAIRDLTRVSRP"
     gene            complement(344..529)
                     /locus_tag="VFA02_10685"
     CDS             complement(344..529)
                     /locus_tag="VFA02_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZU10354.1"
                     /translation="MSTTAKRVKDARKPKFSSRRHNRCKLCGRPRAYLRKFGMCRLCF
                     RQLALKGEIPGVVKSSW"
     gene            complement(549..1097)
                     /gene="rplE"
                     /locus_tag="VFA02_10690"
     CDS             complement(549..1097)
                     /gene="rplE"
                     /locus_tag="VFA02_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZU10355.1"
                     /translation="MAARLKEKYQKEIKPALQKELGLENPMAVPRIEKIVLNMGLGEA
                     TQNNKLIDPLVADLAAITGQKPVLTKAKKSIAAFKVRSGMAIGAMVTLRGDTMYEFLD
                     RLITVALPRVRDFRGVSTRSFDGRGNYTLGLRDQLIFAEIDYAKVEKLKGMNVTIVTT
                     ARDDNSARALLRQFGMPFRQTA"
     gene            complement(1205..1525)
                     /gene="rplX"
                     /locus_tag="VFA02_10695"
     CDS             complement(1205..1525)
                     /gene="rplX"
                     /locus_tag="VFA02_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZU10356.1"
                     /translation="MNIQRNDQVEVIAGSDKGKRGRVLRVFPDNDRILVEHVRVVKKN
                     VRPNPQRNIKGGIAEQEAPIHISNVLLVCPSCGPTRVGHKFEGETKVRVCRKCGQTLV
                     AKKK"
     gene            complement(1562..1933)
                     /gene="rplN"
                     /locus_tag="VFA02_10700"
     CDS             complement(1562..1933)
                     /gene="rplN"
                     /locus_tag="VFA02_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZU10357.1"
                     /translation="MAVQMRTMLEVADNSGARKLQMILPLGGSTGSKAGLGDVITAAV
                     KEASPDGQVKKGKVVKAVIVRTRKETRRRDGTYIRFDSNAAVLINEAGEPVGTRVFGP
                     VARELREKKFLKIVSLAPEVL"
     gene            complement(1953..2270)
                     /gene="rpsQ"
                     /locus_tag="VFA02_10705"
     CDS             complement(1953..2270)
                     /gene="rpsQ"
                     /locus_tag="VFA02_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZU10358.1"
                     /translation="MATATTQSEPRKESRRQEKVGTVVSTKMQKTIVVEVEMRKAHPK
                     YKRIVRQSKKFYAHDEQNSARVGDMVRIRETRPLSKLKRWQLEEIIRRSALAQVEEAP
                     AAE"
     gene            complement(2274..2546)
                     /gene="rpmC"
                     /locus_tag="VFA02_10710"
     CDS             complement(2274..2546)
                     /gene="rpmC"
                     /locus_tag="VFA02_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZU10359.1"
                     /translation="MELEKIRNLSDSELRVEEQKAAEQLFRLRFQIKLGQNEGVKKLR
                     ELKKDIARIKTIARERELGLHGAAPKAESSAPAKPAKKKSTKKEAR"
     gene            complement(2605..3039)
                     /gene="rplP"
                     /locus_tag="VFA02_10715"
     CDS             complement(2605..3039)
                     /gene="rplP"
                     /locus_tag="VFA02_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZU10360.1"
                     /translation="MLMPKKVKYRKQQKGKMRGKAWRGSELSFGDYGLKVMECGYITD
                     RQIEASRIAMTRFIKRGGKVWIRIFPDKPITKKPAEVRMGSGKGPLDHWVAVVRPGKI
                     LFEMEGVAPDVAAEAMRLAANKLPLKTRFVQRHTVQAVVAAK"
     gene            complement(3164..3835)
                     /gene="rpsC"
                     /locus_tag="VFA02_10720"
     CDS             complement(3164..3835)
                     /gene="rpsC"
                     /locus_tag="VFA02_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZU10361.1"
                     /translation="MGQKVHPYGFRIGINKPWRSRWFVERDYDKLLVEDVQLKKELKD
                     KLKAAGVSSVEIERPGNKLRIIIRTARPGIIIGRKGAEIEKLKVDLQKRTNREVFIDI
                     LEVNKPELDAQLVAESIALQLEKRVGFRRAMRKAVDSALRFGCKGIKVRVSGRLNGAE
                     IARSEWYLQGRLPLHTLRADIDYGFAEARTTYGVIGVKTWIYRGDIYQQKRREPQAAV
                     GTSVF"
     gene            complement(3852..4373)
                     /gene="rplV"
                     /locus_tag="VFA02_10725"
     CDS             complement(3852..4373)
                     /gene="rplV"
                     /locus_tag="VFA02_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZU10362.1"
                     /translation="MQLDTREFRAEARFQRVSPQKARLVLDLIKGRGVEEALNTVTFT
                     KKAVAPMVEKVLRSAIQNANYLSQEQGLDVDVDNLYVKQAIANEGPRMKRIRPAPMGR
                     AYRYQRRLSHIIISVAERRQDGLVQTVEAEAAPAAPAKKAAKKAAARKTAAKKAPAKK
                     AARKTTSKKKATE"
     gene            complement(4411..4698)
                     /gene="rpsS"
                     /locus_tag="VFA02_10730"
     CDS             complement(4411..4698)
                     /gene="rpsS"
                     /locus_tag="VFA02_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZU10363.1"
                     /translation="MARSTKKGPFADGHLVEKIEALNRANDKKVVRTWSRRSTIHPEF
                     VGHTIAVHNGKKFVPVYVTENMVGHKLGEFAATRTFKGHSAKAAETTAKAK"
     gene            complement(4832..5656)
                     /gene="rplB"
                     /locus_tag="VFA02_10735"
     CDS             complement(4832..5656)
                     /gene="rplB"
                     /locus_tag="VFA02_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZU10364.1"
                     /translation="MPIKTYRPITPTLRFQTALVNDDITTDQPYKPLVTIRKRTGGRR
                     NSGDLTIRHHGGGHKRKLRLIDFKRDKFGVPGKVATIEYDPNRSARIALISYADGEKR
                     YILQPVGLKVGQTVMSGPEADILIGNALPLRNIPAGTTVHNVELRPGKGAQMARSAGA
                     QVQLVAKEGDYALLKLPSGETRKVLVDCMATIGQVGNTDHENVSIGKAGRNRWKGIRP
                     TNRGVSMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRNNKRTDAFIVSRRTK"
     gene            complement(5787..6080)
                     /locus_tag="VFA02_10740"
     CDS             complement(5787..6080)
                     /locus_tag="VFA02_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZU10365.1"
                     /translation="MPTTYTVIRRPLITERALGAKETEGTLVFEVAPKATKTEIKQAV
                     EALFKVKVSAVRTANYPGKERRRGRFAGHRPDWKKAYVRLKAGEKMPEYIDNL"
     gene            complement(6080..6748)
                     /gene="rplD"
                     /locus_tag="VFA02_10745"
     CDS             complement(6080..6748)
                     /gene="rplD"
                     /locus_tag="VFA02_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZU10366.1"
                     /translation="MANIDVFNLGGQKVGSLELADEVFAPSNVNEALLWEAVKHYRAS
                     LRQGTHATKNRKLVSGSGKKLWKQKGTGRARIGSVRSPLWRHGGTVHGPQPRSYDYAF
                     PKKKLLGALRAALAAKLQDGQITVVESLDLKEPKAKLYRQALNKLEAKKTALLVENSK
                     ELSPNTILGSRNLKGVELVLNNEVHPYDLLRYERAIFSKAALEQMQDALKKTVSRRRK
                     AEVA"
     gene            complement(6821..7570)
                     /gene="rplC"
                     /locus_tag="VFA02_10750"
     CDS             complement(6821..7570)
                     /gene="rplC"
                     /locus_tag="VFA02_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZU10367.1"
                     /translation="MAVTGIIGKKIGMTQVFDEKGEVHPITVLQAGPCVITQLKTAAK
                     DGYDAAQIGLIEFVKASKITKPMQGHFAKNNVPPVKMVKEVPVELAAAKKAEDGQESG
                     NGLKAGDKVLVDIFADEKYVDVVGTSKGRGFAGVVRRHHFGGGPKSHGHMFQVQGSIG
                     ASSFPSRVFPGQRMPGHMGVDRVTVRNLRIRGIDLDENLIMVEGAVPGPRDGYVVISK
                     AKAPPRERRGFAGAGTVDPLKASKKASAKKK"
     gene            complement(7678..7995)
                     /gene="rpsJ"
                     /locus_tag="VFA02_10755"
     CDS             complement(7678..7995)
                     /gene="rpsJ"
                     /locus_tag="VFA02_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZU10368.1"
                     /translation="MVGQRIRIRLKAYDYRVLDTSTGEIVDTAKRTGAQVAGPIPLPT
                     VKNKYCVLRSPHVDKKSREAFEIRTHKRLIDILEPTQQTVDALMKLDLPAGVDVEIKA
                     FEK"
BASE COUNT         1455 a         2674 c         2087 g         1963 t
ORIGIN      
        1 gggcgcgata cgcgcgtaag gtcacgaatg gcggcctcat tgttcgcacc gtacttcagg
       61 tacacacgca gaacagcgcg gccgttctcg tccgaggtct tatagttggc gatatagccc
      121 tcttccttca ggatgcgggc aatctcggtc ttcagcttcg aggccgggac atccagcttc
      181 tggtgacgcg cgcggattgc attgcgcagg cgcgtcagaa aatctgctac tggatcggta
      241 aagctcatcg tttctccttc atcccccgtt cgctctgctt ttcagagaac ctgcgggaat
      301 ggttgttgtg cgccaaaggc gcgtttgcct gacgcggcca gcgtcaccaa cttgatttca
      361 ccactccggg aatttctccc ttgagcgcca gttggcggaa gcacagacgg cacatgccga
      421 acttacgcag gtaagcccgc ggacgtccgc acagcttgca gcggttgtgt ctgcgggaag
      481 aaaacttcgg tttgcgtgcg tctttgactc gctttgcagt ggtagacata tctggtcctc
      541 aatcttcctt acgccgtttg acggaacggc attccgaact gccgcagcag ggcgcgcgcc
      601 gagttgtcat ccctcgccgt tgtcacaatc gtgacattca tgcccttcag cttctcaacc
      661 ttcgcgtaat caatctcggc aaaaatcaac tggtcgcgca gaccaagcgt gtagttgccg
      721 cgtccatcaa agctgcgcgt ggaaacaccg cggaagtcac gcacgcgagg cagcgcaaca
      781 gtaatcaggc ggtcgagaaa ttcatacatc gtatcgccgc gcagcgtcac catcgcgcca
      841 atcgccatac ccgagcgcac tttaaacgcc gcaatggact ttttggcctt cgtcaacacc
      901 ggcttctgtc cagtgatggc cgccaggtcg gcaaccagcg ggtcaatcag cttgttgttc
      961 tgcgtggcct cgcccagtcc catgttcagg acgatcttct cgatcctggg caccgccatt
     1021 gggttttcaa gccccagctc cttctggagc gccggcttga tctccttctg gtacttctct
     1081 ttgagtcttg ctgccatttt tgcttcttct cttttcttct ccggtctcgg agtggccaga
     1141 ggccgtgtac cgttacggag gaactggcca tccaggcaat cgcgccttcg gcgcagccag
     1201 acggctattt cttcttcgcc accagcgtct gtccgcactt gcggcagaca cggactttgg
     1261 tttcgccttc aaacttgtgg ccaacgcgtg tcggaccgca gctcggacag accagaagca
     1321 cgttggaaat gtggatcggc gcttcctgct cggcaatgcc acctttgata ttacgctgcg
     1381 ggttcggacg cacgttcttc tttacaacgc gcacgtgctc gaccagaatg cggtcattgt
     1441 ccgggaaaac gcgcagcacg cggccgcgct tgcccttgtc actgcccgcg atcacttcga
     1501 cctggtcatt gcgctgaata ttcaaactca taaaggtcct ctttatctca tctgccctgg
     1561 actaaaggac ttcaggggca agcgaaacaa tcttcaaaaa cttcttctca cgcagctcgc
     1621 gggccaccgg gccaaagacg cgcgttccaa ccggctcgcc tgcttcgtta atcagcacgg
     1681 cggcattcga atcaaagcgg atataggtcc catcgcggcg gcgcgtctct ttgcgcgtgc
     1741 gaacgatcac ggcctttacc acttttccct tcttcacctg accatcgggt gaagcttctt
     1801 tcacggccgc ggtaatcaca tcgcccaggc cggccttgct gccggtcgag ccgcccagcg
     1861 gcaggatcat ctgcagcttg cgcgcgccgg agttgtcggc gacctcaagc atggtcctca
     1921 tttgcactgc catcgtcgta tctccttatt tcttactcag cggcgggcgc ttcttctacc
     1981 tgcgcaagcg ccgagcggcg gatgatctct tccagttgcc agcgcttcag cttgctcagc
     2041 ggacgcgtct cacgaatgcg gaccatgtcg cccacacgcg ccgagttctg ctcatcatgc
     2101 gcgtaaaatt tcttgctctg gcggacgata cgcttgtatt tggggtgcgc cttgcgcatt
     2161 tccacttcga ccacgatcgt cttctgcatt ttggtcgaaa ccaccgtgcc gaccttctcc
     2221 tgacggcgtg attccttgcg gggctcgctc tgtgttgttg ctgtggccat ggtttagcgg
     2281 gcctccttct tcgtagactt tttcttcgct ggcttcgcag gcgcgctcga ctcggccttc
     2341 ggagcggcac catgcaggcc cagctcgcgc tcgcgggcaa tggtcttgat gcgcgcgatg
     2401 tctttcttca actcgcgcag tttcttcacg ccctcgttct ggccgagctt tatctgaaag
     2461 cgcagacgga acagttgttc agccgccttc tgctcctcga cgcgcagctc gctgtcgctc
     2521 aggttacgga tcttttccag ttccatttct cttcgtcttc tctccgggac aacggcccac
     2581 cgtaggccgc gtctcccaaa atctttactt tgctgccacg acagcctgca ccgtatggcg
     2641 ctgcacaaaa cgggtcttga gcggcagctt gttggcggcc agacgcatcg cttctgcggc
     2701 gacgtccggc gctacgcctt ccatctcaaa caaaatcttg cccggacgca caacggcgac
     2761 ccagtggtcg agcggcccct tgccggatcc cattcggact tccgccggct tcttggtgat
     2821 gggcttgtcc ggaaagatcc ggatccagac cttgccgcca cgcttgataa agcgcgtcat
     2881 cgcaatacgg cttgcttcaa tctggcggtc ggtgatgtaa ccgcactcca tcaccttcag
     2941 gccgtagtcg ccaaatgaca gctccgagcc gcgccacgcc ttgccgcgca tcttgccctt
     3001 ctgctgcttg cgatacttga ccttctttgg catcaacata atgaaaccct cgatagcttt
     3061 tagctattag ctgctagctt ctagccgaat tcttaactcg ccaatcccct ccgctctgct
     3121 taacagtgtg ctaaaagcta agagctagag gctaaaagct gctttaaaat accgacgttc
     3181 ccaccgccgc ctgcggttca cgccgcttct gctggtagat atcgccgcga taaatccacg
     3241 tcttgacgcc aatcacgcca tacgtggtgc gcgcttcggc gaatccgtag tcaatgtcag
     3301 cgcgcagcgt gtgcagcggc agacggcctt gcaggtacca ctcggagcgg gcgatttctg
     3361 cgccgttcag acggcccgaa acacgcacct tgatcccctt gcagccgaag cgcagcgccg
     3421 agtccaccgc cttgcgcatc gcgcggcgga aacccacgcg tttctcaagc tggagagcga
     3481 tgctctcggc aaccagttgc gcatccagct ccggcttgtt cacctcgagg atgtcaatga
     3541 agacctcgcg gttggtgcgc ttctgaaggt ctaccttcag cttctcaatc tccgcgccct
     3601 tgcgcccgat aatgatgcca ggacgcgcgg tgcggatgat aatgcgcagc ttgttgcccg
     3661 gacgctcaat ctccactgag ctgacccccg cggccttcag cttgtccttc aactctttct
     3721 tgagttgaac atcttcgacg agcagcttgt cataatcgcg ctcgacaaac cagcgtgaac
     3781 gccacggctt gttaatgccg atccgaaatc cgtaaggatg gactttctgt cccataattc
     3841 cctctcgccc cctactcagt ggccttcttt ttggaggtag tcttccttgc tgccttcttt
     3901 gccggtgcct tcttcgcagc agtctttctg gccgccgcct tcttggccgc tttcttcgcc
     3961 ggagcagccg gggccgcttc tgcctcgact gtctgcacca ggccgtcctg cctgcgctcc
     4021 gccaccgata tgatgatgtg cgacagacgg cgctgataac ggtaggctcg acccatcgga
     4081 gcggggcgaa tacgcttcat gcgcggtcct tcgttcgcaa tcgcctgctt gacatagagg
     4141 ttgtccacat ctacatcaag cccctgctcc tggctcagat agttcgcgtt ctggatggcc
     4201 gaacgcagca ccttctccac catcggggcc actgccttct tcgtgaaggt caccgtattc
     4261 agcgcctctt cgacgccgcg gcccttaatc agatcgagca ccagccgcgc cttctgcggc
     4321 gatacccgct ggaagcgcgc ttcagcccgg aattcccgtg tgtccaattg cattggcttg
     4381 cctcaaacct tcttcaatct ctgccaaaag ttacttcgcc ttggctgtcg tctccgcagc
     4441 cttcgccgag tgtcccttaa acgtgcgcgt tgccgcgaac tcacccagct tgtgacccac
     4501 catgttctcc gtcacataca ccggcacaaa tttcttgccg ttgtggaccg caatcgtgtg
     4561 ccccacaaac tcaggatgga tggtcgagcg gcgcgaccag gtgcggacga ccttcttgtc
     4621 attcgcccgg ttcagcgctt cgatcttctc caccagatgc ccatctgcaa acgggccttt
     4681 ttttgttgac cgtgccataa aacctctttt agctctcagc tactagccat ttgcttccag
     4741 ccgaatccgg ccagtactta tttcttgtca ctcaacaacc tgctcccgcc acattgagct
     4801 aaaagctggt agccaggagc tgaaagctgc cttattttgt tctgcgactg acgataaatg
     4861 cgtccgtccg cttgttgttg cgcgtcttgt atccgcgtgt cggttggccc cacggcgtta
     4921 ccggatgacg tccacctgag gtcttgccct caccaccgcc gtgcgggtgg tctaccgggt
     4981 tcatcgagac accgcggttc gtcggacgaa tgcccttcca gcggttcctt cccgccttgc
     5041 caatcgaaac attttcgtgg tccgtgttgc ccacctgtcc aatggtggcc atgcagtcca
     5101 caagcacttt ccgtgtttcg ccggaaggca gcttcagcag ggcataatca ccctctttgg
     5161 ccaccaattg cacctgcgcg ccagccgagc gtgccatctg cgcgcctttg cccgggcgca
     5221 gttccacgtt atgcaccgtc gtaccggccg gaatgttacg cagcggcagc gcattcccta
     5281 ttaggatgtc ggcctcaggc ccactcatca ctgtctgccc gaccttcagg ccgacaggct
     5341 gcagaatgta gcgcttctca ccatcggcat agctgatcag ggcaatacgc gcagaacggt
     5401 tcggatcgta ctcaatcgtg gcgacctttc ccggcacgcc gaacttatcg cgcttgaaat
     5461 caatcaggcg cagcttgcgc ttatgtccgc cgccatgatg acggatggtg aggtcgcccg
     5521 agttgcggcg tccgcccgtg cgcttacgga tggtaaccag cggcttatac ggctggtctg
     5581 tcgtgatgtc gtcattgacc agcgccgtct ggaaccgcag cgttggtgtg atcggtctgt
     5641 atgtcttgat cggcatcgtt tagctcttag cttctagctg ccagcctctg gctttgcttg
     5701 cagctcgttc tacttactca atttcaacca ggtttctcat ccagcgctcc agctagaagc
     5761 gaaaagctaa tagctggtga ccgcttttac aggttgtcaa tgtactccgg catcttctcg
     5821 cctgccttca gacgcacata cgccttcttc cagtcaggac gatgtccggc aaaccgccca
     5881 cggcggcgtt ccttgcccgg atagttcgcg gtccgaacag cggagacctt cactttaaag
     5941 agcgcctcta cggcctgctt gatctccgtc ttggttgcct tcggcgcgac ctcaaacacc
     6001 aaagtgcctt cggtttcttt tgcgccaagc gcccgttcgg taatcagcgg gcggcgaatc
     6061 actgtatatg ttgtcggcat taggccacct ccgcctttct gcgccgggaa actgtcttct
     6121 tcagcgcgtc ctgcatctgc tccagggccg cctttgaaaa aatcgcacgc tcgtaccgaa
     6181 gcaggtcata aggatgcact tcattgttga gcaccagctc cacacccttc aggttgcgag
     6241 aacccagaat cgtgttcggg ctaagctctt tgctgttctc gaccagcagc gctgtcttct
     6301 ttgcttccag cttgttcagc gcctggcggt acagctttgc cttcggttcc ttcagatcaa
     6361 ggctttcgac cacggtaatc tggccgtcct gcagcttcgc ggccagcgca gcgcgcagcg
     6421 cgcccaacag cttcttcttc gggaaagcgt agtcatacga ccggggctgc ggtccgtgca
     6481 ccgtaccgcc atgccgccaa agaggtgagc ggacggaacc gatacgcgcg cggcctgttc
     6541 ccttctgctt ccagagcttc ttgcccgagc cggaaaccag cttgcggttc ttggtagcgt
     6601 gcgtaccctg gcgcagcgaa gcgcgatagt gcttcaccgc ttcccacaga agcgcctcat
     6661 ttacattact cggcgcaaag acttcatcgg ccagctccag ggagcctacc ttctgtccac
     6721 cgagattgaa aacgtcaatg tttgccattg tcttaactct ttcagctacc aactcctggc
     6781 tggccgcttt tccgctgacc gcttttcgct atcagctgcc ctactttttc tttgccgaag
     6841 ccttcttcga tgccttcagc ggatccaccg tgcccgctcc tgcaaatcca cggcgttcgc
     6901 gcggcggagc tttggccttt gagatcacta cgtagccatc acgcggcccc ggcactgcac
     6961 cctcgaccat aatcaggttc tcgtcgagat caattccgcg aatgcgcagg ttgcgtactg
     7021 tcacccggtc cacgcccatg tgcccaggca tacgctgtcc cggaaaaaca cgcgacggaa
     7081 atgaagacgc gccaatcgag ccttgcacct ggaacatgtg tccatgcgac ttgggaccgc
     7141 cgccgaagtg atgacggcga acaacaccgg caaagccacg gcccttgctc gttcccacca
     7201 cgtccacgta cttctcatcc gcgaagatgt ccaccaggac cttgtcgccg gccttcaggc
     7261 cgttgccgct ttcctgcccg tcttcagcct tctttgcagc tgccagctcc accgggactt
     7321 ccttcaccat cttcaccgga ggaacattgt tcttcgcaaa atgcccctgc atgggcttcg
     7381 taatcttgga ggccttcaca aattcaatca ggccgatctg cgcagcgtca tagccgtctt
     7441 ttgctgccgt cttcaactgc gtaatcacgc agggaccggc ctgcaggacg gtgatcgggt
     7501 gaacctcgcc cttctcatcg aagacctgcg tcatgccgat ctttttgcca ataattcctg
     7561 taactgccat cgtcttctct cctcatcatg cgccggaaac acagacgcac tgccgggttg
     7621 gctttcagct tttagctctt ggcctccggc gaaaagccag gggccagcag cgaatagcta
     7681 cttctcgaac gccttgatct ccacatccac gccggctgga agatcgagct tcatcagagc
     7741 gtccacggtc tgctgggtcg gctccagaat gtcaatcagg cgcttgtgtg tgcggatctc
     7801 aaaggcctcg cgcgatttct tgtccacgtg cggcgaacgc agcacgcaat acttgttctt
     7861 caccgtcggc aacggaatcg gcccggcaac ctgcgcgccc gtacgcttcg cggtgtctac
     7921 aatctcgccc gtcgaagtat cgagcacccg gtaatcatag gccttcagcc gaatgcgtat
     7981 tctctgtcct accatctcgt tccaatcttc ctgcgggcca gcagttcatt ccgccggccc
     8041 gccactgcta acttctcccc gcgccgagcg cgcgtcgccc tggacggcca gtcctttact
     8101 gcaggatctc ggtaatcgtc ccggcgccta ccgtgcgtcc gccttcccgg atcgcaaacc
     8161 gcagcccctt ctccatcgc
//
LOCUS       DASRBV010000291         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_480704, whole genome shotgun sequence.
ACCESSION   DASRBV010000291 DASRBV010000000
VERSION     DASRBV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480704"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..657)
                     /gene="alkB"
                     /locus_tag="VFA02_10760"
     CDS             complement(7..657)
                     /gene="alkB"
                     /locus_tag="VFA02_10760"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HZU10369.1"
                     /translation="MSLSLFDPNCSELQKEEIVPGAIFLHCFALSGEKAILQEMGEVA
                     ASAPFRNMITPGGHRMSVAMTNCGELGWISDKSGYRYSPVDPESGKKWPAMPESFLRL
                     ATSAAREAGFPDFLPDACLINRYEAGTKLSLHQDRDERDFSQPIVSVSLGIPAIFLFG
                     GLKRTDKTERIPVQHGDVIVWGGPARLRFHGILPLKAAQHPVLGGHRINLTFRKAG"
     gene            794..928
                     /locus_tag="VFA02_10765"
     CDS             794..928
                     /locus_tag="VFA02_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10370.1"
                     /translation="MPNTLGILLLFALVFLAAILWATHGHTADAEGMGDVTGHPGKDS
                     "
     gene            995..2254
                     /locus_tag="VFA02_10770"
     CDS             995..2254
                     /locus_tag="VFA02_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZU10371.1"
                     /translation="MNTTTEEIRQLEPPAIEQGPRPHSQEDNEISLLDLLIVLAERKG
                     TIARITFGLALIALIVSLFLPKSYTATTIVLPPQQSSSLSSALMSQLGGLGSLGALAG
                     SSMGLKNPNDMYVAMFKSRTVEDAMVQRFDLMKEYHQKYLSTARKVFEDHAKVESGTK
                     DNLITISVEDRDPKRAADMANAYVDEYRHLSQHLAIGEAAQRRLFFEQQLEQAKDNLA
                     NAEEALKETQQKTGMIQLDSQARALIESAASLRAQIVAKEVQLQAIKSYATDQNAELV
                     QTQKQLDALRAQLAQLGGDTEEGSGLIVPKGKVPQVGLEYIRKYRDVKYYETIFELLA
                     RQYELAKLDEAREGALIQVVDPAVVPDRKSFPKRGLITIIAAFAGLIIGIFVALVQEG
                     LSRLKEDPEQAQKLNALQAALRKKKTA"
     gene            2300..2845
                     /locus_tag="VFA02_10775"
     CDS             2300..2845
                     /locus_tag="VFA02_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HZU10372.1"
                     /translation="MEPDEVHELHEHHEQAEKDRSLRPVTFTMSLLAVLVAICTVLGH
                     RTHSEAILAQTKASDKWSEYQAKKIRQNETQLTSDMLSTLAAHDPNAQRLVESYKQHA
                     EKWTADLNDEQNEAHKLEAEVDRAEQRGSRFDFGEVLLEVALVITSITLLTRQRSFWY
                     AGLVLGAIGVVICASAYFLPG"
     gene            2948..3682
                     /locus_tag="VFA02_10780"
     CDS             2948..3682
                     /locus_tag="VFA02_10780"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU10373.1"
                     /translation="MKKPDNFHRSGRADKTREKILRSAIKEFSARGLSGARMDAIAKT
                     SRVNKALLYYYFKSKEALYTAALKEVSGRVSETALAAITHPSSPGERLLRLALDHFDR
                     ILTQRDFQNLMQQEMVRFRLGESSALPILVKSAFQPIHEKVEGAVLEGITTGELCDMD
                     PLQVMYSMFGANVFYFLSAPIMRLIVPFDPFSVDAVKHRREALLKFLGQALFQDRRHG
                     AELAKQVLANVPVPEIKPFSARRKRL"
     gene            3679..4743
                     /locus_tag="VFA02_10785"
     CDS             3679..4743
                     /locus_tag="VFA02_10785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10374.1"
                     /translation="MNARNKILVLMGILLIVAVGWYYFSTQRTSDLQLIGTVDANEVV
                     VSSRIPGRIHQLFVDEGDDVKAGQLIATIESEDLAAAKKAAAATALGQRYRLAGAADS
                     ARQTLQGTRDQVASAEAQVNAAKAALAQAQANAARSQTDLQRYTALVQKDVISKQQYD
                     QALAAAKADQAQVESAKANVTAAEAALKVAQANTIQAEVAEKTVAATRSEMQNAQALL
                     NQAQTQLDYAQVVTPISGKVNVRAARQGEVVSAGTPIVTITDLTQTWVYAPLPETEAD
                     AVQVGDMLRVVMPSGATVQGKVIEKSAEGDFATQRDVSRRKRDIKTIRLKLLIENPGM
                     RYVPGMTAEVYIPKDKLVHK"
     gene            4740..5747
                     /locus_tag="VFA02_10790"
     CDS             4740..5747
                     /locus_tag="VFA02_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU10375.1"
                     /translation="MSDSRPAIAVENIVKRYGDFEAVGGITFEVADGEIFGLLGPNGA
                     GKSTLIRMMTTLVPLTEGKACISGHDVSREPDAVRRLIGVIPQALTSDLDLTVEENLS
                     IYAKLYEVPRAARQRNIKELLEAVDLLKWRNAQTKTLSGGMRRRLEIARGLVHNPKIF
                     FLDEPTTGLDPVSRVAVWEMLQKLKNSRNLTILLTTHYMEEADRLCDRIAIVDHGKLV
                     ALDTPMALKASVPGTNVVEAQFATDSPEWPSRLERLQDVTSVEAQGSGMYRILTSNGP
                     QTTTQLVAMAASEGTAIKSLNVQSTTLDDVFVHYTGRQLRDEQVKAFGFMMPQRPGVQ
                     P"
     gene            5744..>6528
                     /locus_tag="VFA02_10795"
     CDS             5744..>6528
                     /locus_tag="VFA02_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10376.1"
                     /translation="MKRTWAIVEREMRKFFRSPALMMVSMTLPLVQLVILGNAFGGKI
                     RGARMGVVDYDHGSQALKIHEAFNAIAANIRTFTTVPYDDEKKAQEDVRTGKIDGAVI
                     IPAQYSRRVLAGDSPTIGLVVDNTDQTMSNALEAEMQSLVNALNAPLIQPRVVQQITL
                     DIVELYPYVEYMKYLLAGSITLAMYVAVMIGGGMLYIDDKARGVHEGYLVTPITRLEL
                     VLGLNLAGALKAVISGVSLTVIGTLLAGLGVIFHPITLLQLSLL"
BASE COUNT         1578 a         1767 c         1833 g         1350 t
ORIGIN      
        1 ccggtgctat ccggctttgc gaaaagtaag attgatgcgg tggcccccca acacaggatg
       61 ttgcgcagct ttcagtggca gaattccatg aaaccgcagc cgcgccggac cgccccagac
      121 aatgacgtcc ccatgctgga cgggaatgcg ttcggtttta tcagtgcgtt taaggccgcc
      181 aaagaggaag atagcaggaa taccgagcga aaccgaaacg atcggctggc tgaagtcacg
      241 ctcatcccgg tcctggtgga gagacagctt cgtccctgcc tcatagcggt tgatgaggca
      301 ggcatcaggc agaaaatccg gaaatcctgc ttcgcgggca gcgcttgtag caagacgcag
      361 aaaggactcc ggcatggctg gccacttttt gccgctttcc ggatctacag ggctgtagcg
      421 atagcctgat ttgtcggata tccagccaag ttctccacag ttcgtcatgg ccacagacat
      481 ccggtgccct ccaggcgtga tcatgttgcg aaatggcgca gatgcagcga cttcgcccat
      541 ctcctggaga atggccttct caccagacag cgcaaagcaa tgaagaaata tcgcgccggg
      601 cacaatctcc tctttttgca gttccgaaca gtttgggtca aagaggctga gactcatggc
      661 ttgctatagt ttgacgcgaa attcttttcc gcagcatttt ttatctggta aattcggcgt
      721 ctccaaaatg agatacgcta ctgcaaccag aatgcttctg cacagattct catctctgca
      781 ggaggtaagc aatatgccaa atacgcttgg aatcctcctc ctcttcgcac tggtttttct
      841 ggcggccatt ctgtgggcca cgcacggaca tacggcagac gcagagggca tgggcgacgt
      901 aaccggacac cccggtaagg attcctgagg cgtgttcttc ttcgcccggt ttctggtagc
      961 ctgaaagaag caccatagcc cgaactggca gcgaatgaac acaaccaccg aagaaatcag
     1021 gcaactggag ccgccagcca tcgaacaagg ccccaggccc cactctcagg aggacaatga
     1081 gatatccctc ctcgatctgc tgattgtcct tgcagaacgg aaagggacga ttgccagaat
     1141 tacattcggt cttgcactga ttgcgttgat tgtctctctc tttcttccaa agagctacac
     1201 cgccacaacc atcgtactgc cgccacagca gagttcctcg ctgtcctcgg cactgatgtc
     1261 acagcttggc ggacttggct cgctgggtgc ccttgcagga tccagtatgg ggttgaaaaa
     1321 tcccaacgat atgtacgtag cgatgttcaa aagccgcacg gtggaagatg ccatggtgca
     1381 gcgctttgat ctgatgaagg agtaccacca gaaatacctt tccactgcgc gtaaagtatt
     1441 tgaagaccat gccaaagtgg agtccggcac aaaggacaac ctcattacca tctccgtgga
     1501 agaccgcgac ccgaaacgcg cagcagacat ggccaatgcc tatgttgatg aataccggca
     1561 cctttcacag cacctggcta ttggagaagc agcgcagcgg cgtttgtttt tcgagcagca
     1621 actggagcag gccaaagaca atcttgccaa tgcagaagaa gcgctgaagg aaacgcagca
     1681 gaaaacgggc atgattcagc tcgacagcca ggcgcgcgcg cttattgagt cagccgccag
     1741 tctgcgtgcc cagatcgtgg ccaaagaagt acagttgcag gccatcaaaa gctatgcgac
     1801 ggaccagaat gccgagctgg tccagacgca gaaacaattg gatgcccttc gcgcgcaatt
     1861 ggcgcagctt ggcggtgata cagaggaagg cagtggcctg attgttccta aaggaaaggt
     1921 gccacaggtt ggcctcgaat atatccgcaa gtatcgcgat gtaaagtatt acgagactat
     1981 tttcgagctt ttggcgcggc agtatgaact ggcaaaactg gatgaagccc gggaaggcgc
     2041 attgattcag gttgtggacc cggccgttgt tccggaccgc aagtcttttc ccaagcgcgg
     2101 cctcattaca atcattgctg ccttcgctgg tcttatcatc ggcatctttg ttgcgttggt
     2161 gcaggaaggt ctttcacgtc tgaaggaaga ccctgagcag gcccaaaagc tgaacgcctt
     2221 gcaggctgct ctccgaaaaa agaagactgc atgatgcgcc aacagtcctg cacgcctgtt
     2281 gctgtaaagt aagaacgcaa tggaacccga cgaagtacac gaactgcacg aacatcatga
     2341 acaggctgag aaggaccgca gcctgcgccc ggtgaccttt actatgagcc tgctggctgt
     2401 tctggtggcc atctgcaccg tcctgggcca tcggacgcac tccgaagcaa tcctggcaca
     2461 aaccaaagcc agcgacaaat ggagtgaata tcaggccaag aaaatccgac agaacgaaac
     2521 acaactgacg tcagacatgc tttccactct ggccgcccac gaccctaacg cgcagaggct
     2581 tgtggagagt tacaaacagc atgccgaaaa gtggacagca gacctcaatg atgagcagaa
     2641 tgaggcgcac aaattagaag ctgaagtgga ccgcgccgag cagcgcggca gccgttttga
     2701 ttttggcgaa gtgctgctgg aggtcgcgct cgtgattacg tctattacgc tgctgactcg
     2761 acagcgctcc ttctggtacg caggtctggt ccttggcgcc attggcgtgg ttatatgcgc
     2821 aagcgcttac ttcctgccag gctgagaaag gtccccaccg aatcttttat attcgccggc
     2881 tcctttttag ggaagtggtt tgtgttttgc cctcttcccc gtctatgatt aactaaccgg
     2941 ttaatcaatg aagaaaccag acaacttcca tcgaagtggg cgcgccgaca aaacgcggga
     3001 gaagatcctg cgctctgcga tcaaagaatt cagcgcccgg ggcctgtccg gggcacgcat
     3061 ggatgcgatt gcaaaaacgt ccagggtcaa taaagcgctg ctctattact acttcaagag
     3121 caaggaagcc ctctatacgg ctgcgctcaa agaggtttca ggtcgtgttt cagagaccgc
     3181 actcgctgca attacgcacc cttcaagtcc tggagaaaga cttctccgcc tggcgctgga
     3241 ccactttgac cgcatcctca cgcagcggga cttccagaat cttatgcagc aggagatggt
     3301 gcgttttcgt ttgggcgaaa gctccgcgct gcccatattg gtgaagtctg catttcagcc
     3361 catccacgaa aaagtggaag gcgctgtgct tgagggcatc acgaccggcg agctatgcga
     3421 catggacccg ttgcaggtga tgtattccat gttcggggcg aatgtctttt attttctgag
     3481 cgccccgatc atgcggttga ttgtaccttt tgaccccttc agtgtggacg cggtaaaaca
     3541 ccgccgcgag gcgctgctga agtttctggg gcaggcattg tttcaggacc gcaggcatgg
     3601 cgcagagctg gccaagcagg tgctggccaa tgttcctgtg cctgaaatca aaccattttc
     3661 tgcccggaga aagcgtttat gaatgcgcgt aacaaaattc tggtactgat gggaattttg
     3721 ctgatcgtcg ccgtcggttg gtattacttt tccacgcagc ggacaagcga tctgcaattg
     3781 atcggcaccg tggatgccaa cgaagtcgtc gtcagctcgc gcattcccgg acgcatccac
     3841 cagctatttg tagatgaggg tgatgacgtc aaagcaggac aactgatcgc cacaatcgag
     3901 agtgaggacc tggccgcagc aaaaaaagca gcagcagcaa cggccctggg acagaggtat
     3961 cgccttgcag gcgcggccga tagtgcgcgc cagacgctgc agggaacaag agaccaggtc
     4021 gccagtgccg aagcccaggt caacgccgca aaggcggccc ttgcacaggc gcaggccaat
     4081 gcagccagat cgcaaacgga ccttcagcga tataccgcgc tggtccagaa agatgtgatc
     4141 tcaaaacaac agtatgacca ggccctggcc gccgccaaag ctgatcaggc gcaggtggaa
     4201 tcagcaaagg cgaatgtgac cgcggcagaa gcagcgctca aggttgcaca ggcgaacaca
     4261 atccaggcgg aggtggcaga gaaaaccgtg gcggccacga gaagcgagat gcagaatgcc
     4321 caggcattgc tgaatcaggc gcagacacag cttgattacg cgcaggtagt tacgcccatc
     4381 tcagggaaag taaatgtaag agcagcgcgt cagggcgaag tggtttctgc aggaacaccc
     4441 attgtgacca tcactgacct gacgcaaacc tgggtctatg ctcccctgcc ggaaaccgaa
     4501 gctgatgctg tgcaggttgg agatatgctg cgggtcgtga tgccaagcgg cgcgaccgtt
     4561 caaggcaagg tcattgaaaa atctgccgaa ggcgattttg ctacgcagcg cgacgtaagc
     4621 cgccgcaagc gcgacattaa aaccattcgc ctgaagttgc tcatcgagaa ccccggcatg
     4681 cgttatgtgc ccggaatgac agccgaagtg tacatcccca aagacaagct ggtgcacaaa
     4741 tgagtgattc tcggccagct attgctgttg aaaatatcgt gaaacgctat ggcgattttg
     4801 aagcggtcgg tggcattacg tttgaggtgg cggacgggga aatctttggt ctgcttgggc
     4861 cgaacggcgc gggcaagagc acgctcattc gcatgatgac caccctcgtt ccgttgacgg
     4921 aaggaaaagc ctgcatcagc gggcatgacg tatcgcgcga accggatgca gtgcgacggt
     4981 tgattggggt catcccgcag gcgctcacca gcgacctgga tttgaccgtc gaggaaaatc
     5041 tttccatcta cgccaagctt tatgaagttc cgagggcagc gcggcagcga aatatcaagg
     5101 agctgctgga agccgtagac ctgctgaagt ggcggaatgc gcagaccaaa acgctctccg
     5161 gaggcatgag gcggcggctg gaaattgctc gtgggctggt ccacaatccg aaaatctttt
     5221 ttctcgatga accaaccacc gggctggatc ctgtctcgcg cgtggccgtg tgggagatgc
     5281 tgcaaaaact caagaacagc cgtaacctga ccatcctgct gacgacccac tatatggaag
     5341 aagcagaccg cctgtgtgac cgcatcgcca ttgtggacca cggaaagctg gtagctctgg
     5401 acacgcccat ggccctcaag gccagcgtgc ccgggaccaa tgtggtagag gcgcaattcg
     5461 caaccgattc gcctgaatgg ccttcgcgtc tggaacgtct gcaagacgtg acttccgtag
     5521 aagcgcaagg atctggcatg tatcgcattc ttacgtcgaa tgggccgcaa acgacgacac
     5581 aattagttgc aatggcagcc agcgaaggaa cagcgattaa atcgctcaac gtgcaaagca
     5641 ccacgctgga cgacgtcttt gtccattaca cagggcgaca actacgcgat gaacaagtga
     5701 aggcatttgg atttatgatg ccgcagcggc cgggagtgca gccatgaaac ggacttgggc
     5761 gattgtagag cgcgagatgc ggaagttctt ccgttcacct gcgctgatga tggtttccat
     5821 gaccctgccg ctggtgcaat tggtgattct aggcaatgcc tttggcggca agattcgtgg
     5881 cgcgcgcatg ggcgtggtgg attacgacca cggatctcag gcgctgaaaa tccacgaggc
     5941 gttcaatgca attgcagcca acatccgcac ctttacaact gttccgtatg atgacgagaa
     6001 gaaagcgcag gaagacgtgc gcaccggcaa gattgatggc gctgtgatta ttcctgcgca
     6061 gtattcgcgc cgcgtccttg ccggcgactc tccaaccatt ggccttgtcg tggacaacac
     6121 cgaccagacg atgagcaatg cgcttgaagc agagatgcag tcgctggtga atgctctgaa
     6181 cgcgcctctg atccagccca gagtcgtgca gcagatcacg ctggatatcg ttgaacttta
     6241 cccctatgtc gagtacatga aataccttct ggccggatcc atcacactcg ccatgtatgt
     6301 ggccgtcatg atcgggggag gcatgctcta cattgatgac aaggcccgcg gcgtccatga
     6361 aggatatctg gtcaccccca tcacccggct tgagttggtg cttggcctga atctcgccgg
     6421 cgctttgaag gccgtaatct ccggcgtaag ccttactgtg atcggcacgc tgctcgcagg
     6481 gctgggagtc atcttccatc ccattacgct tctgcaactc tcgcttct
//
LOCUS       DASRBV010000292        31227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_732570, whole genome shotgun sequence.
ACCESSION   DASRBV010000292 DASRBV010000000
VERSION     DASRBV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 31227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31227
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732570"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFA02_10800"
     CDS             complement(<1..810)
                     /locus_tag="VFA02_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10377.1"
                     /translation="MFRRSSFFVFAAALFALGFAGLLGHAQDYRATITGTVTDSSKAV
                     IADATVSVRDLDTNQTVTVHTNSAGVYVVPYLTPGQRLEVSAEAPGFKKMTYPPVVLT
                     VSQKQTADFVLQVGSATQEVTVNSESYQIGLDTEKADRGTVIDNKTITQLPLNGRNPL
                     SLMDYIPGVTNEAGPGLESPPNNMYNISFFTVNGTPAQNTEYQIDGMPNNANPWYSNG
                     PSTVPSVDAIQEFKVVTNPYDAEYGRTSSGVVSMELKSGTNQLHGSAYEFAK"
     gene            1142..2368
                     /locus_tag="VFA02_10805"
     CDS             1142..2368
                     /locus_tag="VFA02_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZU10378.1"
                     /translation="MLKMEEVDGLRDALSALLLLPEHEKQARGLTDTPREIQQQPGTW
                     LGTLERLKAVQTSTREFLESCGLLNGLPGQPDVILVGAGTSDYIGRALVRVLRQQWRC
                     HVSAVPSTELLTNMEDHVLPGRQYLMISFSRSGQSSEGIAVMEQALQCCSGHVHHLVV
                     TCNESGPMAKFPGVFTITLDDAVNDRGLAMTSSFSNMVVAGQYLAYLRSPERYGPLLK
                     NMVSMGADLLPEAANLAAGLAKKKFSRVCFLGSGALQAVAHESALKVMELNAGKIATL
                     AESFLGLRHGPMSFLNQETLVTAFLSGNEDRVLYELDLLEEIRNKHLAGDILIVAPRV
                     TSRIQQLTEHILPLHASVGFPDACRPPVDVIAGQLLALFFAIENGITPDTPSDGAISR
                     VVSHVKIYSPAGRTGK"
     gene            2365..4350
                     /locus_tag="VFA02_10810"
     CDS             2365..4350
                     /locus_tag="VFA02_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HZU10379.1"
                     /translation="MTKVKCAPVCAVFLAASLYAQDAGKAIASVHGNSYRLSNHEVEA
                     SWNITDGKLDGITVTDRLNGEVIRLADPFAVLLKDGTIYNAGNVRVDGTPEMHALKPQ
                     PDASRYSDRIAGEEFDLPLVSQDGNLHLKWAVVLRDGSNYMRQLLTLTAGGHDLPITR
                     VQLMDVDLPGARVDGSVKGSPIVAGTVFLGFEHPLSESKVVKDRATAYLDRELPLRAA
                     QPVTYSSVIGVTRPGQLRRDFLNYIERERAHPYRTFLHYNSWYDLGYFNPYDEAGALD
                     RINTFGRELHDKRGVTLDSYLFDDGWDDHSSLWSFNKGFPNGFTPVRAAAEKYGAEPG
                     VWMSPWGGYDGPKKERVKFGKEQGYEIVDNGFALSGPKYYARFRDVCMEMIRKYGVNQ
                     FKFDGTGNADSVFPGSAFDSDFSAAIHLIGELREARPDIYINLTTGTYPSPFWLLYAD
                     SIWRGGWDDNVAGVGPYRERWITYRDADTYDEIVKGGPLYPLNSLMLHGIIYAQHHKH
                     LNADPGNDFRNEVRDYFGTGTQLQEMYITPSLLTQQNWDDLAEAAKWSRANADVLKDT
                     HWIGGDPAWLEVYGWASWTPKKGILVLRNPDDKPQSIRLDVQDAFELPSGAPQTYSAK
                     SPWKDDAAKPAIELRAHTPHEFQLAPFEVLTLDVTPR"
     gene            complement(4420..6591)
                     /locus_tag="VFA02_10815"
     CDS             complement(4420..6591)
                     /locus_tag="VFA02_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HZU10380.1"
                     /translation="MAKTAVSTVEREKAAPSGLAPDRLIEMYRLMYLSRRTDDREIML
                     KRQQKIFFQISGAGHEALLVAAGMALRPGYDWFFPYYRDRALCLTLGVTVEDMLLQAV
                     GAAADPSSGGRQMPSHWGSRKLNIVSQSSATATQILHAVGCAEAGRYFSRHPEAAEKH
                     EGDYRAFKDISFYGDEVVYASLGEGSTSQGEFWEAINTASNEKLPVLFVVEDNGYAIS
                     VPVEVNTPGGNISKLVANFPNFYFAEVDGTDPIESFRVFEKAVAHCRSGAGPAFVHGH
                     VIRPYSHSLSDDDRLYRPKSELEADALRDPIHKLQMYLLREGILDADAINRLEKQVDE
                     EVRLAAERALRAPFPEPSTIPLHVYSEDYDPTRSGLETKPAQTADPQERTMADLINAC
                     LRDEMRRDPRIVIFGEDVADCSREEYLKEGLVKGKGGVFKLTAGLQAEFGSDRVFNSP
                     LAEANIVGRAVGQAVRGVKPVVEIQFFDYIWPATHQMRNELSVMRWRSNNAFSAPAVI
                     RVAIGGYLTGGAIYHSQCGESIFTHTPGIRVVFPSNALDANGLLRTAIRCDDPVLFLE
                     HKRLYRETFGRAPYPGPEYMVPFGKAKTVREGKHLTIVTYGAVVPRALQAAQKAQREH
                     SIETEILDLRTLSPYDWEAIAASVKKTSRVIVAHEDMLSWGYGAEIAARIADELFEDL
                     DAPVKRVAALDTFVAYQPLLEDVILPQPERIYQAITELARF"
     gene            6928..9192
                     /locus_tag="VFA02_10820"
     CDS             6928..9192
                     /locus_tag="VFA02_10820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZU10381.1"
                     /translation="MSFSLLVTACFLQANDVSTSSSDSLYLWVSLVVGVVGLLAAFGF
                     ARSVLGSDSGSPEMQRISNAIRQGAEAFMKRQYGTIAMIAVVLAIILFIGYRLSPFTA
                     PYANKVVISFLIGAACSAISGQTGMWVSIRSNIRTAAAARTSFSRALQIALRGGAVTG
                     LIVVALSLLGVGVLFLLFGGLHDPQQAPYQLVGFGFGASLVALFAQLGGGIYTKAADV
                     GADLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGRGADIFESTAAENVGAMILGAA
                     LYPVFGIKGILFPLIVQAINLIASIVGVAVVRSKEDEEPMHALNRGYYVTTILALIGF
                     AAAIHFMLQGRWWLLGCGICGIVTSFLFVRITEYYTETRFRPVRSIALASTTGPATNI
                     ISGLAVGMETPAVPVIVISAALLLSYYFGTLGLADVGTISSYAKGIYGTAIATMGMLS
                     SAAYILAMDTFGPITDNAGGIVEMSNQPDNVREKTDKLDAAGNTTKALTKGYAIGSAS
                     LAAFLLFSAYLESVHDIVSQRAAAAGDALSSTWSFSNVNLASVPVFVGALLGAMLVYL
                     FSSLAIKAVGRAAQAVIKDVHQQFRENPGIMEGTSQPDYGRCVGIVTKSALKEMVLPG
                     ALAVLMPLAVGVIFRHFSTSFRLSEAASAPVINGHVLNLGGAEAVAALLMVGTISGIL
                     MAMLMNNGGGAWDNAKKFIETGQYGGKRSEAHKAAVVGDTVGDPFKDTAGPSLHVLIK
                     LLATVTLVLAPLFV"
     gene            9292..10476
                     /locus_tag="VFA02_10825"
     CDS             9292..10476
                     /locus_tag="VFA02_10825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZU10382.1"
                     /translation="MDAGRKIPWHQRRRESFRYYRSVPFSRLWSLFGAVFCLFGVVGV
                     FNDLMNLGRGPYVILTVGIVLSGLTAVIYLFIAVRLKAYFYIIVSALQVPFWIAFGYF
                     EPIIVRRFRLHENAPEDGVRVAAIAMFALVIASYVLFIRFLRGEGRESVRIRNELELA
                     HGIQKTLVPTISLHTAQFELYGRSDPSDKVGGDLVDTVQLSDGRVVAYLADIAGHGLQ
                     AGILMGMLKTATRTALLDAHAAEPCQVLSSMMKRLNQVLPDVKEPQMYATFAGFQLRD
                     DGIVEYALAAHPPVLLYRGRSGVEQLSSEQFPLGLLSVDGFFSESTVMNSGDLLIAVT
                     DGILEACNELEEEFGLERLQSLVASRAKDTLPALGNAILGTVRAFGRQTDDQTLLLIR
                     KL"
     gene            complement(10473..11030)
                     /locus_tag="VFA02_10830"
     CDS             complement(10473..11030)
                     /locus_tag="VFA02_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HZU10383.1"
                     /translation="MSADPVRNALKRGTTLGIIWAVILVLCGIAALALPVVAGISAAI
                     FLAILILISGVIHLTTAPVAGGFGGYLWRTLVGIVYIVGGIWIFMHPVLGLVSFTLVL
                     GWMFLFEGILYLLSYFQARRTGGAGWLLVDGIVTLVLALLILVHWPSSSAWAIATIVG
                     VNLLISGFTRLMALSAVRAALNATD"
     gene            complement(11091..11498)
                     /locus_tag="VFA02_10835"
     CDS             complement(11091..11498)
                     /locus_tag="VFA02_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10384.1"
                     /translation="MRYFHASFGWQALTLSLVLLTGDFAVLPAQQQQQQIPQVPTVSR
                     PGLPGQPASPDADTGPDPLARRIMLEQAKKRNQQRQQDIVNDTNKLLQLAQQLKTEVD
                     KSNQDQLSLSVIKKAEEIEKLAKSVKEKMKGNS"
     gene            11601..12518
                     /locus_tag="VFA02_10840"
     CDS             11601..12518
                     /locus_tag="VFA02_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZU10385.1"
                     /translation="MDDLLNFAYTALPKIVVTIIAAWIFARILKAFTHRMVRITERHA
                     ASASRIAQVKTLASVIRATGIGFIIFVAALEVLPLFGFKLEPLLASAGVAGVAIGLAA
                     QTIVKDCLNGVLILIEDQYNVGDKVRIAGVAGIVESLSLRKTLVRDGDGTLYVVPNSQ
                     ITTVANLTRDYSVATINVSVDFSADPDKVMKLLKDVAMSVRNDPEYKDIFLADPTLLG
                     VDTIKGSQVIYPVQLKTIADKQWAAMRETQRRIRIALEKNGLLPGDPLRVYGTGAAGQ
                     HLPNNEAIVAADPTTAKANEVNPFTGEGM"
     gene            complement(12499..13011)
                     /gene="coaD"
                     /locus_tag="VFA02_10845"
     CDS             complement(12499..13011)
                     /gene="coaD"
                     /locus_tag="VFA02_10845"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZU10386.1"
                     /translation="MNTVKAIYPGSFDPVTNGHLDLIARGSKIFDHLVVAILRNSGKN
                     PLFSVEERVEMLSEALAGFHNVSVATFNGLLVDFVREQHAQAVMRGIRAISDYEYELQ
                     MALMNRRLAPDLETVFLMPDGKYSFVSSRLVKEVFTLGGSIEGLVPQFVIERLKARVP
                     NGHEPTSLPR"
     gene            complement(13309..13890)
                     /locus_tag="VFA02_10850"
     CDS             complement(13309..13890)
                     /locus_tag="VFA02_10850"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZU10387.1"
                     /translation="MPYTIESIRNASSYVWISLCAVWLLLSFSTKQTVRKQTAESRIR
                     YVVLLVLGCFLIFYKGPANGWFDTRLFPVTPATALVGFIMVCLGIAFSIWARLILGRN
                     WSGVVTIKEDHSLIRRGPYSIVRHPIYTGILLGMLGSAIQYGLLRSLLGVALLGFGFW
                     IKSLTEEHFMVQQFGQEYLRYREQVRALVPFVF"
     gene            complement(14003..15385)
                     /locus_tag="VFA02_10855"
     CDS             complement(14003..15385)
                     /locus_tag="VFA02_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZU10388.1"
                     /translation="MTTLDDLHTAAEDIFRHALEACDIGKSFDRHLHFEDATLVRHPA
                     ALLKPVSIDLRNYKKVVVIALGKAALTMLDTLLQRLPKKIHLRGVCSAPEIPKKRNWR
                     IRYFTGGHPLPNKHSFDAAEAALKLLRRARKDTFVFFLISGGGSAMFELPRDPRISLE
                     DTIAFHETLIASGATITEINTVRKYFSAVKGGRLALAAPEAEKLSLLLADVPMKDLGA
                     VASSPTLPDQSTLEECREILARYQLLEKFPSAVRDYFETLSPEIVAETHNTAAAEAFR
                     HTQFDTLLSNHDFVNAARDRAHALGFRVVIDNTCDDWDYADAARYLLKRLHDLRAEHP
                     RLCLLSSGEVTVQLGASPGCGGRNQQFALAAALDLAKHEGQPLVVLSAGSDGIDGNSP
                     AAGAIADTTTVSRARAYHFNPEDNLARFDSCPLFTALGDTIITGPTGNNLRDLRILLA
                     VREETTQAAS"
     gene            complement(15382..16047)
                     /locus_tag="VFA02_10860"
     CDS             complement(15382..16047)
                     /locus_tag="VFA02_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU10389.1"
                     /translation="MTTIHSGTATLRTVDDLRGPAGKLEALLNAGAPDAQYAALVCHP
                     HPLGGGTMHNKVVYHAMKALQALGLPVLRFNFRGTGLSEGKHDEGRGEQDDVRTALDW
                     LEREFRLPVLFAGFSFGAAVGLRACCGDARVKGLIALGLPTHAEGRDYQYHFLASCTQ
                     PKLFISGTRDQYAPIEQLEEAFGIAAHPAEIVWIDGAEHFFAGKLDEVQLAIRNWVQA
                     HFS"
     gene            16077..17078
                     /locus_tag="VFA02_10865"
     CDS             16077..17078
                     /locus_tag="VFA02_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU10390.1"
                     /translation="MTFDPVQFTRRLIDIESITYNEGAAGAFLADFLAQRGFSIEKMP
                     VESPERFNVYACVKGQTPDIVLSTHMDTVPPFIPSSEDDEFIYGRGACDAKGIIAAQI
                     GAAERLRDSGVKVGLLFLVGEERDSAGAKVANTQPKGSRFLINGEPTDNRLAIASKGA
                     LRAAVRAKGKMAHSAYPELGESAVHKLVEALNRVLEIRLPVTEDIGPSTLNIGLLEGG
                     RAPNVIADHAEAQVLVRLVGSSEETRKALEEAVAGLAEVEFTLEIPFMRMRALDGFPT
                     MVAAFTTDIPALTNWGEPVLLGPGSIHVAHTPHEKLAKRELLEAVDLYIEVAKRLVG"
     gene            17087..17563
                     /locus_tag="VFA02_10870"
     CDS             17087..17563
                     /locus_tag="VFA02_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10391.1"
                     /translation="MMTRAARWRKVTRAVLLGWTVAFLATSPFQICEVWRNSGRSLTL
                     LVSALGYGFAVWFLLTLAGALLTWICIATPIAIFIHPQRLLRRRGLVMGLSIAAALLV
                     VAYQLHIWTHWYHDGVGLMNFAIYALFATVFSGTAAYAYLRSLESSWTRSQASDHS"
     gene            17582..19690
                     /locus_tag="VFA02_10875"
     CDS             17582..19690
                     /locus_tag="VFA02_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU10392.1"
                     /translation="MVQLNALEHAASSYLRSAMHQPVQWHEWSPEAFALAAKEDKPIL
                     LDIGAVWCHWCHVMDRESYEDPTLAQVINEHYVAIKVDRDERPDVDSRYQAAVQAMSG
                     QGGWPLTAILTPDGKPFFGGTYFPRDDRYGRPGFERVLRTLADVWKMKRADALETAQS
                     VIAAIEHGETFSGRASSLTPELIDKLVVSSVSQFDPRYGGFGSQPKFPHPAAMDLLLD
                     VAARGNEGAKKVATFTLEKMAAGGIYDQLAGGFHRYSVDERWVVPHFEKMLYDNSELL
                     KNYVHAFQSFVQPEFARVAKDIMRWMDEWLTDRERGGFYASQDADISLDDDGDYFTWT
                     RKEAAAVLSSDELEVAGLYYDIGEMGDMHHNPEKNVLHVEYSLEQVAQKLSRDVKDVE
                     RDLVSAKKKLHAARLQRPAPFIDKTIYTGWNALAVTAYLEAAHVLHLPAARVFALKTL
                     DRILDEGWNTQEGLTHVIAYADGVAAKQRVAGVLDDYAFLIHACVDAWQATGVLRYYT
                     AAMEIAGVMVTRFYDATGGGFFDTEHSKDEAIGALSARRKPLQDTPTPAGNPAAAAAL
                     LRLEALSGRSDFREKAEDTLEHFGGIVEHFGLFAGTYGLALERLLLPPVQVVVIGNDE
                     QAQNLSAMATARYAVNKSIIALRQEQVRAENLPPVLAETLPEIPNLQSGGSFAVVCKG
                     NACQPPTNDAEELLRELSEA"
     gene            19730..20590
                     /locus_tag="VFA02_10880"
     CDS             19730..20590
                     /locus_tag="VFA02_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZU10393.1"
                     /translation="MIVRLVRKAILCCLLVPVSLGAMAYAESSVEQALRQAVHGTPAV
                     AVVLDVKNGRTIAAVGANEGAMLRTAPGSTLKPMFLSYALGHRFIQPQTTAFCHRGLR
                     IAGRNLDCTHPQSEKVFNAELALAYSCNNYFAEMARRFTPEQAEDALREFGLTGQIES
                     GASIEKLQFFVLGLEGIRVSPDTLARAYRKLAAQWNAGLPDAVKRGLEDSVQYGMAHN
                     AYVPGVRVAGKTGTASETGQPWTHGWFAGIVPADHPKIVIVVYLPRGNGADAAHLAQH
                     FFSAYKDAPR"
     gene            20587..22284
                     /locus_tag="VFA02_10885"
     CDS             20587..22284
                     /locus_tag="VFA02_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZU10394.1"
                     /translation="MKAVLIYILLIAGGACAQNTNGPAALTVRLFSSHRADAVTFVPL
                     SINSKIKTCAKCADRPLHGPVTLKNTQMKPGVRVVLRGAFRASIDNAEQSETAAGEWT
                     VTPAKNGLRVLLTTTAEHYVMAALSGEAAPDEPLESLKAMAVAARTFAIVDQHRHEAE
                     GFDLCDSTHCQALRFGHVRPEIEQAVRATAGETLWFGAHRANVYMTQHCGGETEDASS
                     VWPAVRAPYLRAHADPYCLRRSQAKWYADIQVPQLARILYGEGWKIPAQIDSVQVVKR
                     TAAGRAQMLQITGQGESTRISASSFRFAINRSLGWNQLRSDWYEITRNNNMLHFEGKG
                     YGHGVGLCQAGAFEMAKEGHNYREILGFYFPGTQVRVTAKDQGWKQVGGQGWTLMALG
                     GVDIVNAGNAAWTKAQAVFSPKSSVHPVVYLMPSTELFRQTTDEPGWMLASTRGTDVY
                     LQPVAIMRAHRGASSVLLHEFLHVLVEQEATAKAPLWLREGLVEALAGDIHAREVLAP
                     KAAMLDAGLAHPVSLAESQRAHFAAGELVQKLIQQYGLNTVRGWLQGGVPAAVVAEN"
     gene            22301..22660
                     /locus_tag="VFA02_10890"
     CDS             22301..22660
                     /locus_tag="VFA02_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10395.1"
                     /translation="MRVRTIFAAVMVVLLIAGMTTSAMCEMTCLPKAQTAACCPHHAQ
                     QMVMHCQQHSETASFVNAHTCEHPQDSAAVAACIVAAPSVISGVIIGTNLQRAPVFYA
                     HSSSTPILKPLHPPLRI"
     gene            22733..23980
                     /locus_tag="VFA02_10895"
     CDS             22733..23980
                     /locus_tag="VFA02_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10396.1"
                     /translation="MTRVFVLLLVLCGVAAAQDMSEMMDRGNHSFTQQIVHHATSGTS
                     AEPNSTPVPMWMRMQGDWMLMFHANAFVMDEQQTSPRGGDKFFSTNWFMPMAQRQMGP
                     GYLTLRGMFSLEPATITQRRYPLLFQQGETAYGKAIVDGQHPHNFLMELAAIYDWKIS
                     EKTLLTFYAAPVGDPAIGPTAYPHRASAFENPVGTLGHHQEDSTHIADDVVTAGFTHG
                     LARIEASGFHGREPNEHRWQVQQGAMDSWSTRLTLQPGQNWSGQYSYGRIHSPEALFP
                     SEDQERMTASVMYNRPLKNGNWASTLAWGRTRALTDNVIQNSYLLESTLQFAKTNYVW
                     TRMENVDRTTELLHDAGPEEPAGRVQAYTFGYDRDFRTWPHWATALGAQVSVYSPGSR
                     LQPVYGTDPMGVVVFLRLRPSGK"
     gene            24024..25349
                     /locus_tag="VFA02_10900"
     CDS             24024..25349
                     /locus_tag="VFA02_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10397.1"
                     /translation="MAIANQQKKTSIKDFSHAWRALRHRNFRLFFGGQSISLIGTWMT
                     RIATSWLVYRLTKSSLLLGTVSFAGQIPTFLLAPFAGVLVDRMDRRKVLVWTQVLAMI
                     QSLALAGLTLAHMINIREVLVLSVFQGMINAFDMPGRQAFMVQMVEDRADLSNAIAIN
                     SSMVNTARLVGPSLAGLVIAATNEGWCFLIDGISYIAVIASLLIMRMRAGQEKRAAAS
                     MIEQLKEGWAYVSSFVPIRMILLLFALISLMGMPFVVLMPVFAAQVLHGGPHTLGFLM
                     GALGVGALISALSLVVRKSVRGLSKMIPIAAAVFGAGLIAFGFSTNQWLSLLLMLFTG
                     FGMMQGMTASNTIIQTLVPEDKRGRVMSYYTIAFVGMAPFGSLLAGGLAHAIGAQETV
                     VISGIACMAGALWFWSHLKDVRKEMRPIYEQLGILQPKVPIVVEEEVSR"
     gene            complement(25409..26296)
                     /locus_tag="VFA02_10905"
     CDS             complement(25409..26296)
                     /locus_tag="VFA02_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU10398.1"
                     /translation="MTATKTISGAAAGTLTLGGDLTIHRMGYGAMRITGHGIWGPPAD
                     KAAALATLRRAVELDVNFIDTADSYGPNVSEELIAEALYPYPKGLVIATKGGWLRVGP
                     GQWIHNASPKHLIEAAEGSLKRLRLDRIDVYQLHVPDPAVSFDASMEALARLREQGKV
                     RHVALSNVTLEHIERARKIVPIVSVQNRYSFADREWDYVLDFCERNGIAFLPWAPLGQ
                     GRKAHEAIEKVAAELGASTRQVALAWLLKRSSVMVPIPGTSSVAHLEENIAAASLELP
                     EDKFAGLSAVNPPPASLRG"
     gene            complement(26313..26975)
                     /locus_tag="VFA02_10910"
     CDS             complement(26313..26975)
                     /locus_tag="VFA02_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZU10399.1"
                     /translation="MSDQILSAEDRARRIKIILFDVDGVLTDGGIWIFPAPAPSQTTA
                     AHAQEMESKGGYAIHSANMVEAKGFNAHDGSGVSLARLGGMKCGIITKRISETVALRA
                     RDLRLEYVYMGQAFKMQAVREIMAKESVTLEEIAYVGDDVIDLPVMREVGLAIAVANA
                     RKQVKDAAHYITPNFGGHGAGRDAVEFILEAKGILEEVIEKYIDERNPIAKSMDIGQG
                     GM"
     gene            27223..27435
                     /locus_tag="VFA02_10915"
     CDS             27223..27435
                     /locus_tag="VFA02_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HZU10400.1"
                     /translation="MKIIGILLIALGVIALIYGGITYTTHKKVIDMGPIQAEKKETHN
                     VPLPPVLGVIAIVGGGALLVFGAKAR"
     gene            27575..30583
                     /gene="secA"
                     /locus_tag="VFA02_10920"
     CDS             27575..30583
                     /gene="secA"
                     /locus_tag="VFA02_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZU10401.1"
                     /translation="MIGSALTKVFGTSNERAVKRLMPVVQAINAMEPDVQRLSDGELR
                     AKTDEFKVRIAKALEGIEDAEERKAAEKRALDEVLPEAFAVVREAGRRVLNMRHFDVQ
                     LIGGMVLHQGKIAEMKTGEGKTLVATLPCYLNALAGHGVHVVTVNDYLAKRDAEWMGK
                     IYEFLGLTVGVIVHDLDDNQRRQAYSADITYGTNNEFGFDYLRDNMKFELADCVQRGH
                     YFAIVDEVDSILIDEARTPLIISGPTDQTTDKYVRVDRIIPQLVQGEEIEQGVDKEKI
                     LTGDYVVDEKHKTISVTDEGWEKIEKLLGIGNIADPENWEYKHHVETAIKAHSLYKRD
                     VQYVVKDGPQGKEVIIVDEFTGRLMPGRRWSDGLHQAIEAKEGVNIRREDQTLATITF
                     QNYFRLYQKLAGMTGTAETEAAEFDKIYKLEIVVIPTNKPLLRIENPDVVYRTAKEKY
                     FAVADEIARLHEKGQPVLVGTTSIEKSELLSQILQRKGVKHVVLNAKYHEREAEIVAQ
                     AGRLGMVTIATNMAGRGTDILLGGNAEFVAKQELVKKGLARAISVAEGALNPMAAPGM
                     LRFYYQGQEFETSQENWDKTYAVHAAKAQEEHDAVVAAGGLHIIGTERHESRRIDNQL
                     RGRAGRQGDPGASRFYLSLEDDLMRIFAKEWVGTLLQRLGMEEGVPIESRMISKRIEA
                     AQKAVEAQNFEARKHLLEYDDVMNKQREAVYGLRHQLLEGLDQKELIIEDYVATILSN
                     LLDEHTPEKIHPEQWDLKGLKEKLISHFGFDPEAEGINLGEMTRHEIGETIFERLKER
                     YEAKEQIIGDKQMRFHERMIMLSVLDGLWKDHLYNMDHLKEGIGLRGYGQQDPLVAYK
                     KESFDMFEGMMARFQEDTVRFLFHMQIVGPDGRPLQVPSRPRPSVPAAPPVASAEAVP
                     QHVSSAVAVAEPPAVPIPVRQPSTTIDEIEREFQRKKQRELEHARMAGTGGDSAAPVQ
                     RRTGDKIGRNDPCPCGSGKKYKKCHGAGEG"
     gene            complement(30711..>31227)
                     /locus_tag="VFA02_10925"
     CDS             complement(30711..>31227)
                     /locus_tag="VFA02_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZU10402.1"
                     /translation="GSSAMPHKRNPITSEQISGLARVVRSNAQAAYENVALWHERDIS
                     HSSVERVIFPDSTILTDYLLAKTTNLLENLLVYPQRMLKNLESTGGLIFSGQLLLDLA
                     EAGMLREDAYRLVQKHAMRAWKEDLVFRDLVKTDPEITAKLAPEKLEKTFDLSRQLRN
                     VDAIFDRVLAS"
BASE COUNT         6724 a         8238 c         9219 g         7046 t
ORIGIN      
        1 cttggcgaac tcataagcag aaccatgaag ctggttggtg ccggacttga gttccatact
       61 gacaacgccg ctggatgtac ggccatattc cgcgtcatag ggattcgtga cgaccttgaa
      121 ttcctgaatg gcatctacgg aagggaccgt tgatggtcca ttggaatacc acgggtttgc
      181 attgttgggc ataccgtcaa tctgatattc agtgttctgc gccggagtgc cgttcacggt
      241 aaaaaacgag atgttgtaca tgttgttcgg tggcgattcc aggcccgggc ctgcctcatt
      301 tgtgacgccg gggatgtagt ccatcagaga cagagggttc cggccattca gcggcagttg
      361 cgttatggtt ttgttatcaa tcactgtgcc gcggtctgct ttttcggtgt ccaggcctat
      421 ctggtagctc tcactgttta cggtcacttc ctgggttgca gaaccgacct gcaaaacgaa
      481 gtccgcggtc tgtttttgcg aaacagtgag aacaaccgga ggataagtca ttttcttaaa
      541 gcctggagct tctgcagaaa cttccagcct ttgacccggc gtgagatagg gcacgacgta
      601 aacaccggca ctgttcgtgt gtacagtgac ggtctgattg gtgtcgaggt cgcgcacgga
      661 aacagtcgcg tctgcaatga ccgccttgct ggaatcggtc actgtgcccg taatggttgc
      721 gcggtaatcc tgggcgtggc ccaacaggcc ggcaaagcca agcgcaaaga gcgctgccgc
      781 gaatacgaag aaactgctac gccgaaacat acttttgcct ccagagtctt gctgaaaccg
      841 ccgagggtat ttccgggaaa ttgctgaatt tccatgaaaa gaccaattac tttcacatct
      901 tttggagcaa aatagaaagt aaaaaataat taaacaaata aaacagtata gtcaatggaa
      961 attttcggaa attatccgga gaatgaaaac ctttgatagg agagggtttc tacacgcgag
     1021 ggataatgag gatgccgagt acagagtgcg attggaggtg aacttcgcct taaaaggcca
     1081 aattctatgt ttctttttat ttcgcttgat tacggtatcc aatatcgtgt aatacaatca
     1141 aatgctgaag atggaagaag tagatggttt gcgggatgcg ctctctgccc tgctgttatt
     1201 gcccgaacac gagaaacagg cccgtggtct gacagacaca ccgcgcgaaa tccagcagca
     1261 gccgggaaca tggctcggta ctctggaacg gctgaaggcc gtccagacct cgaccaggga
     1321 atttctggaa tcctgtggtc tgttaaacgg gctgccaggc cagccggacg tgattctggt
     1381 gggggcgggg acttcggact acattggcag ggcgctggtc cgcgttctgc ggcagcagtg
     1441 gcgttgtcat gtttcagcgg tgcccagcac ggagctgctg accaatatgg aggaccacgt
     1501 ccttcccggc aggcaatacc tgatgatctc cttctctcgt tcaggccaga gctcggaagg
     1561 cattgcagtg atggagcagg cgcttcagtg ctgctcaggg catgtgcatc atcttgtggt
     1621 tacctgcaat gaatccggcc cgatggcgaa gtttcccggc gtgtttacca tcactctgga
     1681 tgacgcggta aacgatcgcg gccttgctat gaccagctcg ttctcaaaca tggtagtagc
     1741 agggcaatac ctggcttatc ttcgctcacc ggaaagatat gggccgctcc tgaagaacat
     1801 ggtgagcatg ggagcagacc tgttgccaga ggctgcgaat ctggctgccg ggttggcgaa
     1861 aaagaagttc tctcgcgtgt gttttctggg cagcggggcc ttgcaggcgg ttgcgcatga
     1921 gtcggcgctg aaggtgatgg aactgaatgc gggtaaaatc gccacgctgg ccgagtcgtt
     1981 tctgggcctg cgccacgggc cgatgtcttt cctgaaccag gaaacactgg tgacggcatt
     2041 tctttccggc aatgaggacc gggtgcttta cgagcttgat ctgctagagg aaatccgcaa
     2101 caaacatctt gccggagaca ttcttatcgt cgctccacgt gtgacgagcc gcattcagca
     2161 actgacagag cacatcctgc cgcttcatgc cagtgtgggg tttccggatg cgtgccgtcc
     2221 tcctgtggac gtgatagcag ggcaactgct ggcgctcttt ttcgccattg aaaacggcat
     2281 tactccggac acacccagcg atggggccat cagccgtgtt gtttcccacg taaaaatcta
     2341 ttctcccgcc ggaaggaccg gaaaatgacg aaagtgaagt gcgcgccggt gtgcgcggtg
     2401 tttcttgctg cctctctcta tgcgcaggat gccggaaaag cgattgcctc cgtccatgga
     2461 aacagctaca ggctctccaa tcatgaagtg gaagcaagct ggaacattac agatggaaag
     2521 ctggacggaa ttaccgtcac agacagattg aatggcgaag tcatacgcct ggctgatccg
     2581 tttgcggtcc tgctgaaaga cgggaccatc tataacgccg gcaacgtgcg cgtggatggc
     2641 acccctgaga tgcatgcact taagccgcag ccggatgcat cgcgctactc agaccgcata
     2701 gccggtgagg aatttgacct gccgctggtg agccaagacg gcaatcttca cctgaaatgg
     2761 gcagtcgtgc tgcgcgatgg ctcaaattat atgcgccagt tgctgaccct cacggctggc
     2821 ggacatgatc tgccgattac ccgcgttcaa ttgatggatg tggacctccc cggcgctcgt
     2881 gtggacggct ctgtaaaagg ctcgcccatt gttgcgggaa cggtcttttt gggattcgag
     2941 catcccctct cagagagcaa agtagtcaaa gaccgggcaa cggcctacct agaccgcgag
     3001 cttccgctca gagctgcgca gccggttacg tattcttctg tcatcggagt gacgcgtcca
     3061 ggccagttgc gccgcgattt tctgaattac attgagcgcg agcgcgcgca tccgtaccgc
     3121 acattcctgc attacaactc ctggtatgat ctgggctatt tcaaccccta tgacgaagcc
     3181 ggggcgctgg accgcatcaa cacgttcgga cgcgaactgc atgacaagcg cggcgtcacg
     3241 ctcgattcgt atctctttga cgatgggtgg gacgatcatt cctctttgtg gagctttaac
     3301 aaaggctttc ccaacgggtt tacgccagtg cgcgcagcag cggaaaagta cggcgcagaa
     3361 ccaggcgtgt ggatgtctcc ctggggcggg tatgatggcc cgaaaaaaga gcgcgtcaag
     3421 ttcggcaaag agcagggcta tgagattgta gacaacggtt ttgcactctc cggacctaag
     3481 tattatgccc ggttccgcga tgtttgtatg gagatgatcc gcaaatatgg cgtaaaccag
     3541 ttcaagtttg atggcacggg gaatgccgat tctgtgtttc cggggagtgc ctttgacagc
     3601 gacttttccg cggcgatcca cctgattggc gagttgcgcg aagccaggcc agacatttac
     3661 atcaacctga cgacgggcac atatccttcg cccttctggc tgctctatgc tgattcgatc
     3721 tggcgcggtg gctgggacga caatgttgcg ggagtcggcc cgtatcgtga gcgctggatt
     3781 acctaccggg atgcggacac ctacgatgaa atcgtgaaag gcggaccact gtatccgctc
     3841 aattcgctta tgctgcacgg cattatttat gcgcagcacc ataagcacct gaatgccgat
     3901 ccgggcaatg acttccgcaa tgaagtgcgc gattactttg gcacggggac tcagttgcag
     3961 gagatgtaca tcactccttc gttgttgacg cagcagaact gggacgatct ggcggaagcc
     4021 gcaaaatggt cacgtgccaa cgcggatgtg cttaaagaca cgcattggat cggaggcgat
     4081 ccggcgtggc tcgaagtcta cgggtgggcc tcatggaccc cgaagaaggg gattctggtt
     4141 ctccgcaatc cagacgacaa gccgcagagc attcgccttg atgtgcagga cgcgtttgaa
     4201 ctgccatccg gagcgccgca gacctattcc gcgaaaagtc cctggaagga tgatgcggcc
     4261 aagcctgcga ttgaactacg cgcgcatact ccgcacgagt tccaactcgc tccgtttgaa
     4321 gtgctgacgc tggatgttac gccgcgataa ggtgcggcat tctcacagac gggtgtggac
     4381 caccccggtc cccatgacct gtgaaaatgc cgccaggaac taaaatctcg ccaactcggt
     4441 aattgcctga tagatacgct ccggctgcgg cagaatcacg tcttccagca ggggctggta
     4501 ggccacgaaa gtgtccaggg cagccacgcg ctttaccgga gcatcgaggt cttcaaacag
     4561 ctcatcggcg atgcgggcgg cgatttccgc gccatatccc caactcagca tgtcttcatg
     4621 cgccacgatg acgcggctgg tcttctttac cgatgcggca atcgcttccc agtcgtaggg
     4681 gctgagggtg cgcaggtcga gaatttccgt ctcaatgctg tgctcgcgct gcgccttctg
     4741 tgctgcctgc aatgcgcgcg gcacaacggc gccgtaggtc acgatggtga ggtgcttgcc
     4801 ttcgcgcacg gtctttgctt tgccgaaggg gaccatgtat tccgggccag gatacggagc
     4861 gcgtccaaag gtttcgcggt agagccgttt gtgttcaaga aagagcacag ggtcatcgca
     4921 gcggatggcg gtgcggagaa gaccattggc gtccagggcg ttggaaggaa agacgacgcg
     4981 gattcccggt gtatgcgtga atatgctctc gccgcactga gaatggtaaa tcgcgcctcc
     5041 agtcaggtaa ccgccgatgg cgacacgaat gacggcaggc gcggagaatg cattattcga
     5101 gcgccagcgc atgacgctca gctcattgcg catctggtga gtggcgggcc agatgtagtc
     5161 aaagaactgg atttcgacga ccggcttcac tccgcgcacg gcctgcccaa cggcgcggcc
     5221 aacgatgttg gcctcggcca gtggggaatt gaacacgcgg tcgctgccga actcggcctg
     5281 gaggcctgct gtgagcttga aaacgcctcc tttgcccttt accagccctt ctttcagata
     5341 ctcctcacgg ctgcaatcgg ccacatcttc gccaaagatg acgatgcgtg gatcgcggcg
     5401 catttcgtcg cgcaggcagg cgttgataag gtcggccatg gtgcgctcct gcggatcggc
     5461 ggtctgggcg ggcttcgttt ccagcccgct gcgcgtcggg tcatagtcct ctgaatacac
     5521 atggagcgga atcgtagatg gctcaggaaa cggcgcacgc agtgcgcgtt cggcggcgag
     5581 gcggacctct tcgtctacct gtttttcgag ccggttgatt gcatctgcgt ccagaatacc
     5641 ttcgcggagc agatacatct gcaatttatg gatgggatcg cgcagtgcat cggcctccag
     5701 ctcgcttttg gggcggtaga ggcggtcgtc atcggagagc gagtgggaat aaggacggat
     5761 cacgtggcca tgcacaaagg ccggccctgc tcccgagcgg cagtgggcca cggccttctc
     5821 aaaaacacga aagctctcaa tagggtcggt gccgtccact tctgcaaagt agaaattcgg
     5881 gaaattggcg acaagtttcg agatgtttcc gccgggcgta ttgacttcaa cagggacaga
     5941 gatagcgtag ccattatctt cgaccacaaa gaggaccggt agtttttcgt tggaggcggt
     6001 gttgatggcc tcccagaact ctccctggct ggtagaacct tctccgaggg acgcatagac
     6061 gacttcgtcg ccgtagaagg agatgtcctt gaaggcgcgg taatcccctt cgtgcttttc
     6121 agcggcttcg ggatggcggg agaagtagcg tcctgcttcg gcgcagccaa cggcgtgaag
     6181 gatctgggtg gctgttgcgg aggactgcga aacaatgttc agcttcctgc tgccccagtg
     6241 tgagggcatc tggcgtccgc cgctggaggg gtcggcggct gccccaacgg cctggagaag
     6301 catatcttcc acggtgacgc cgagtgtaag gcaaagggcg cggtcacggt agtaagggaa
     6361 aaaccagtcg tatccgggcc tgagggccat cccggcagcg accaggaggg cctcatggcc
     6421 ggcgcctgat atctggaaaa agattttctg ctgccgcttg agcatgatct cgcggtcgtc
     6481 tgtccggcgc gaaagataca tgaggcggta catctcgatc aggcggtctg gagcaagtcc
     6541 ggaaggcgct gccttttccc gctccacagt agatactgcc gtcttggcca ttctcaaaaa
     6601 acctcttcag gggctaccat gatcgccagc tttttctcag gcgacaggag attgtacatc
     6661 tttgccgtgc ggttgtcttt gcacagtcaa gccgcacgga accgaccgtt ttatgatgcc
     6721 ttgacctctt tgaatgcagc ggagggtttt gtattcctat tgtaaaagtg tgggcaaaca
     6781 ggcatccaat tgactatcgt tttctccgcg tgtttgaatg cagagttttg tgggcttcct
     6841 gcaagcattt cccaaaggac ggtctgcttt gatacgtttg tcgtttcggg agcaagctgg
     6901 ttccgcatct tttctggagg acattgaatg tcgttttcct tactggtgac tgcatgtttt
     6961 ttgcaggcca atgatgtttc cacctcatcc tctgacagcc tttatctgtg ggtttcgctt
     7021 gtggtcggag tagtgggtct gctggcggcc tttggttttg cccgctcggt gctcggctca
     7081 gactccggct cgccggagat gcagcgtatt tccaacgcca tccgccaggg cgctgaggcc
     7141 tttatgaaga ggcagtacgg aacgattgcg atgattgcgg tggtccttgc cattattctt
     7201 tttattggat atcgcttgtc tccgttcact gctccatatg caaataaggt tgttatcagc
     7261 tttctgattg gcgcggcctg ttcggccatc tccggacaga cgggcatgtg ggtctccatc
     7321 cggtcgaaca tccgtacggc ggcggcggcg cgtaccagct tcagccgtgc gttacagatt
     7381 gctctgcgtg gcggtgctgt gacgggcctc atcgtggttg cgctttctct gcttggcgtg
     7441 ggcgtcctct tccttctgtt tggagggctg cacgatccac agcaggcacc ttatcaattg
     7501 gtgggctttg gttttggggc gtcgctggtg gcgctttttg cccagcttgg cggcggcatt
     7561 tacacgaagg ccgctgacgt gggcgcagac ctcgtaggca aggtcgaagc cgggattccg
     7621 gaggacgatc cgcgcaaccc ggccgtgatt gccgacctgg tgggcgacaa cgtgggcgac
     7681 tgcgccggcc gtggcgcaga cattttcgag tccaccgcgg cggaaaacgt gggcgcgatg
     7741 attctgggcg ccgcgctcta tccggtcttc ggcatcaaag gcattctgtt tccgctgatt
     7801 gttcaggcga ttaatctgat tgccagcatc gtcggcgtag cggtggtgcg cagcaaggaa
     7861 gacgaagagc cgatgcatgc gctgaaccgc ggatactacg tcacaacgat tctggcgctg
     7921 attggctttg ccgcagccat tcattttatg ctgcaagggc gctggtggct gctgggatgc
     7981 ggcatctgcg gcatcgtcac ttcattcctg tttgtccgca tcacggagta ctacacggaa
     8041 acgcgcttcc gacccgtgcg ctcaattgca ctggcctcga ccaccggccc tgcaacaaat
     8101 atcatcagcg gtctggccgt aggcatggag acacccgcgg tccctgtcat tgtgatcagc
     8161 gcggcgctgt tgctgagcta ctacttcgga acgctcggcc tggcggatgt ggggacgatt
     8221 tcctcctatg cgaagggtat ttatggtacg gccattgcca ccatgggcat gctgtcttca
     8281 gcggcctaca ttctggccat ggacaccttc gggcctatta cagacaacgc tggcggaatc
     8341 gtggagatga gcaaccagcc ggacaatgtc cgcgaaaaga cggacaagct ggacgctgcg
     8401 gggaacacga cgaaggcgct caccaagggc tatgccatcg gatcggcatc actggcggcg
     8461 tttctgctgt tctcggctta tctggaaagc gtacatgata ttgtgagcca gagggcagcg
     8521 gccgcaggcg atgctttgtc ttccacctgg agcttctcga atgtgaatct ggcttccgtg
     8581 cctgtgtttg tgggcgcgct gttgggtgcg atgctggtgt atctgttctc atcactggcc
     8641 atcaaggccg tgggacgtgc ggcgcaggca gtcatcaagg acgtgcatca gcagttccgc
     8701 gagaatccgg gcattatgga aggcacatcg cagccggatt atggccgttg cgtaggcatc
     8761 gtgaccaagt ctgcccttaa ggagatggtg ttgccgggtg cgctggcggt gctgatgccg
     8821 ctggccgtgg gtgtcatctt ccgccacttc agcaccagtt tccggctgag tgaggcggcg
     8881 agtgctccgg tcatcaacgg acatgtgctg aatctgggtg gggccgaggc ggtggccgcg
     8941 ctgctgatgg tcgggaccat ctccggcatt ctgatggcaa tgctgatgaa caatggcggc
     9001 ggcgcatggg acaacgccaa gaagttcatc gaaaccgggc aatacggcgg caagcggtct
     9061 gaagcacaca aggctgccgt ggttggcgat acggtcggcg atccgttcaa ggacaccgct
     9121 ggaccatcgc tgcacgtgct gattaagctg ctggccaccg tgaccctggt gctggctccg
     9181 ctgttcgttt agtcaagata gagacaccca catcctgaac agatgtgggt gtctttattc
     9241 cctccagatt gtctttctct tttctgccgg gcgtctgcta tcgttctggc aatggacgca
     9301 ggcaggaaaa tcccctggca tcagcgcagg cgcgaatcat ttcgctacta tcggtcggtc
     9361 ccattcagca gactatggtc attgttcggc gctgtcttct gcctgtttgg tgtagttggc
     9421 gttttcaatg atctgatgaa tctggggcgg gggccttatg tcattcttac ggtgggcatt
     9481 gtcctcagcg gacttactgc ggtcatttat ctcttcatcg ccgttcgact taaggcgtac
     9541 ttctatatca tcgtttctgc tttacaggtc ccattctgga ttgcgtttgg ctattttgaa
     9601 cccataattg tccgccggtt ccggcttcat gagaacgctc cagaggatgg tgttcgagtt
     9661 gccgccattg cgatgttcgc acttgtcatt gcttcgtatg tgctgttcat acgctttctc
     9721 cgtggagagg gccgcgagtc ggtccgcatc cgtaacgagc ttgaactggc gcacggtatt
     9781 cagaagacac tggtgcctac gatttctcta catactgcgc aattcgagct gtatggacgc
     9841 tccgatccaa gtgacaaagt gggcggcgat cttgtggaca cggtgcagtt aagcgatggc
     9901 agagtagtgg cgtatctggc agacattgcg ggacatggtc ttcaggccgg gattctaatg
     9961 ggaatgctga aaactgctac gcggacggcc ctgctggatg cgcacgcggc ggagccatgt
    10021 caggtgttgt cctcaatgat gaaacggttg aaccaggtat tgcctgatgt gaaggaaccg
    10081 cagatgtacg ccacatttgc gggttttcaa ctgagagacg acggtatcgt ggaatatgcg
    10141 ctggctgcgc atcctccggt cctgctttac cgggggaggt cgggcgtgga gcagctttca
    10201 agcgaacagt ttccgttggg gctgttgtca gtagatggct ttttctctga atccacagtg
    10261 atgaattcag gtgacctgct gattgctgtt acagatggaa tccttgaggc gtgcaatgag
    10321 ctggaagaag agtttggcct ggagcggtta cagagtctgg ttgcaagccg cgcaaaggat
    10381 acgcttccag ccttgggcaa cgccatactt ggaacagtac gcgcctttgg caggcagaca
    10441 gacgaccaga cacttttgct catccggaaa ctttaatccg ttgcattcaa tgctgcgcga
    10501 acggcagata gcgccatcag tcgcgtgaat ccgctgataa gcagatttac gccgacaatc
    10561 gtggcaatgg cccacgccga actggaaggc cagtgaacaa ggatcagaag cgccagaacc
    10621 agcgttacga tgccatccac cagcaaccac cctgcgccgc cagtccggcg ggcctgaaag
    10681 tagctaagca gatacaagat gccctcaaac aggaacatcc atcccagcac aagcgtgaaa
    10741 gaaaccaaac caagcactgg atgcatgaag atccagatgc cgccaacaat gtaaacaatg
    10801 ccgaccagcg tccgccacaa atacccgcca aaacctccgg caactggagc cgtcgtcaga
    10861 tggattacac cggagataag aatgaggatg gcgagaaaga ttgcggcact gattccggcg
    10921 acgacaggca gtgcaagcgc tgcaattccg cagaggacaa gaatgaccgc ccagatgata
    10981 ccgagagtcg tcccgcgctt cagggcattg cggacagggt ctgctgacat gcgattcctc
    11041 ctcaggagga taataaccac gtaggacggt tttaaggggc ctcctggccg ttaactattt
    11101 cccttcatct tctctttgac cgatttggcc agtttttcga tctcttctgc tttcttgatc
    11161 acggagagcg agagctggtc ctgattgctc ttgtctactt ctgttttgag ttgctgcgca
    11221 agctggagca gtttgttcgt gtcgttcacg atgtcctgct gtcgctgctg gttgcgcttt
    11281 tttgcctgtt ccagcataat gcggcgggcc aatggatccg gtccagtgtc cgcatcgggc
    11341 gaagcaggtt gtccaggcag accgggccgc gacaccgtag ggacctgggg aatttgctgt
    11401 tgttgctgct gcgcaggcag cacggcaaaa tcgccggtga gaaggaccag cgaaagagtc
    11461 agggcctgcc atccgaaact ggcatgaaag taccgcatca gttcattata ggaatgttca
    11521 ctatggtttt aacagatgca aattctctgt tctcatttgc cagtgattgc agccgcctcg
    11581 cccggttcaa tgccgaattc atggacgatc ttttaaattt tgcctatacg gcgcttccca
    11641 agattgttgt caccatcatc gcagcatgga tttttgcgcg gattttgaag gcgtttacgc
    11701 accgcatggt gcggattacg gagcgccacg cggccagtgc gtcgcgcatt gcgcaggtga
    11761 aaacgctggc ttccgtcata cgcgccactg gtattggctt catcattttt gtggctgcat
    11821 tggaagtcct gccgctgttc ggttttaagc tggaacctct actggccagc gcaggagtgg
    11881 caggcgttgc cattggtctt gcggcacaaa cgatcgtgaa agactgcctg aacggtgttc
    11941 ttatcctgat cgaagaccag tacaacgtag gcgataaggt gcgtattgcc ggagtggccg
    12001 gcattgtcga gtcactttcg ttgcgtaaaa cgctggtgcg cgatggagac ggcacccttt
    12061 acgtcgtccc taattcacaa attacgactg tggccaatct gacgcgcgat tactctgtgg
    12121 cgacgatcaa tgtttcggta gatttctctg ccgatccgga caaagtcatg aagctgctga
    12181 aagacgtggc catgagcgtg cgcaacgatc cggaatacaa ggacatcttt cttgccgacc
    12241 cgacactgct gggcgtggac actattaaag gctcgcaggt gatttatccg gtgcagttga
    12301 agacgatcgc tgacaagcag tgggctgcca tgcgcgaaac gcaacgccgg atccgcattg
    12361 cgcttgaaaa gaacggtctg ctgccgggcg acccgcttcg tgtttacggc actggcgcag
    12421 ccggtcaaca tttgcccaac aatgaggcca ttgtcgcggc tgatccaacg accgctaaag
    12481 ccaatgaggt gaacccgttt accggggaag ggatgtaggt tcatgcccgt tggggacacg
    12541 ggctttcagc cgttcaatta caaattgcgg aacgagtcct tcaatcgaac cgccgagggt
    12601 aaatacttct ttgaccaggc gcgagctgac gaaggagtat ttcccatcgg gcataaggaa
    12661 gacggtttcc aggtcgggag cgaggcggcg gttcatcagg gccatttgca gctcgtactc
    12721 ataatcgctg atggcgcgga tgccgcgcat cacggcttgc gcatgctgtt cgcgcacaaa
    12781 atctacaagc agtccgttga aggttgctac agaaacattg tggaagcctg ccagcgcttc
    12841 agagagcatt tccacgcgct cttccacgga gaacagaggg ttcttgcctg aatttctcag
    12901 gatggcgaca accagatggt caaatatctt cgatccgcgc gcaatcaggt cgaggtgacc
    12961 attcgtgaca gggtcaaagc tgccgggata gatggctttt acggtattca cgccaaaggc
    13021 attgtatcgc cttgtgcttt ttgctgcatc agtgaccgct ttcaggtttg cgggtagccc
    13081 acagaatgca aacagcaaaa accgttacaa agctgaaaat ggaaaaaatt gccaaagtat
    13141 aactcctgaa tgaaaaatca ctgacagcat agcaggagaa ttattgtttc gtcagtagtt
    13201 tttccagtat ggacaacccc ttcagaacga cccgtccttc atttccattt agcaggcaag
    13261 ccctgaaagg gtttatttga attgctgcgc caggtcaacg ggtcaccgtt aaaagacaaa
    13321 tggcacaagc gcgcgcacct gctcgcgata gcgcagatat tcctggccaa actgttgcac
    13381 catgaagtgc tcttccgtaa gggatttgat ccagaagcca aacccgagga gcgcaacgcc
    13441 gagcaggctt cgcaacagcc catattggat ggccgagcca agcattccaa gcaggatacc
    13501 ggtatagatg ggatgccgca cgatactata gggaccgcgc cttatcagcg agtggtcttc
    13561 cttaatggtg acgacgccgc tccagttccg gcccagaatg agccgtgccc agatactgaa
    13621 tgcaatgccc aggcacacca tgatgaaacc gaccagggcc gtggcgggag tgacagggaa
    13681 aaggcgcgta tcaaaccagc cgttggcagg acctttgtaa aaaatgagaa aacaacccag
    13741 aaccagcagg acaacatacc ggattcgcga ttctgcagtt tgctttcgca cagtctgctt
    13801 ggtgctgaag ctgagcagga gccagactgc acatagcgat atccacacat aggaagatgc
    13861 gttacggata gattcgatgg tgtagggcac gggccgtctt tctctttttc tttctatgat
    13921 ttcttcatat aggacgtatc ctatatggca caacgaaatg ctcaggcaaa gaggctgtca
    13981 agaagcttgt gaaaggatga aatcaagatg ctgcctgcgt cgtctcttcc cgcactgcaa
    14041 gcagaatgcg aaggtcgcgc aggttattgc ctgtgggccc ggtgatgatt gtgtctccta
    14101 atgcggtgaa cagagggcaa ctgtcgaagc gggccagatt atcttcaggg ttgaagtgat
    14161 aggcgcgcgc acgcgagacc gtagttgtat ctgcgatggc gccggcagcg ggactgtttc
    14221 cgtcaatgcc atctgatcct gcgctgagaa caaccaaagg ctggccctca tgctttgcga
    14281 gatcgagcgc cgcggcgagt gcaaactgct ggttgcgccc gccgcatccc ggagaagcgc
    14341 caagttgtac tgtgacttct ccgcttgaaa gcagacaaag gcgcggatgc tccgcgcgca
    14401 ggtcatggag gcgtttgagc agatagcgcg cggcgtcggc gtagtcccag tcatcgcatg
    14461 tgttgtcaat cacaacgcga aagccgagcg catgggcgcg gtcgcgcgct gcattcacaa
    14521 aatcgtggtt cgacaagagc gtatcaaatt gcgtgtgcct gaatgcctct gctgccgcgg
    14581 tgttgtgggt ctctgcaacg atttcgggcg acagcgtctc gaaatagtcg cgcaccgcgg
    14641 aaggaaattt ttccagcaac tgataacgcg cgaggatttc gcggcattct tccagagtgg
    14701 attgatcagg caaggttgga gacgacgcaa ctgcgcccag gtccttcatg ggtacgtcgg
    14761 ccaacagaag cgacagtttt tctgcttccg gcgcagccag cgcaaggcgg ccacctttga
    14821 ctgcggaaaa atatttgcgg acagtgttaa tttccgtgat ggttgcgcca gaggcgatca
    14881 gcgtttcgtg aaaggcaatc gtgtcttcga gagaaattct cggatcgcgg ggtagttcga
    14941 acatcgccga tcctccgccg ctgatgagga agaagacgaa tgtgtccttg cgggcgcgtc
    15001 tcagcaactt gagagcggct tcagcagcgt cgaacgaatg cttgtttggc agcggatgcc
    15061 cgcctgtgaa gtaacggatt cgccagttgc gttttttggg tatttcagga gcagagcaga
    15121 cgccgcgcag gtggattttc tttggcagtc gttggagcag cgtatcgagc atggtcaaag
    15181 ccgcttttcc taaggcgatg acgacgacct tcttatagtt cctgagatct attgagaccg
    15241 gcttaaggag tgccgcggga tgacgcacca gcgttgcatc ttcaaagtgc agatgacggt
    15301 caaaggactt gccaatgtcg caggcttcaa gcgcgtgtcg aaaaatgtct tcggctgctg
    15361 tgtgaagatc atcgagcgtg gtcatgagaa atgcgcctgt acccagttgc gaatggccag
    15421 ttggacctcg tccagtttac ccgcgaagaa gtgttctgcg ccgtctatcc atacaatttc
    15481 cgcaggatgt gcggcgatgc cgaaggcttc ttccagttgc tctatggggg cgtattggtc
    15541 gcgtgttccg ctgataaaga gcttgggctg cgtgcaggaa gcaaggaagt gatactggta
    15601 atcgcggcct tctgcgtgag ttggcaggcc cagcgcgatc agtcccttca cacgcgcatc
    15661 gccgcagcag gcgcgcagcc ccacggctgc tccaaaggaa aatcctgcaa acagtaccgg
    15721 aagccggaac tcccgctcca gccaatcgag cgctgtgcgg acatcgtcct gctcgccgcg
    15781 tccttcatcg tgtttgcctt cgcttaatcc ggtcccgcga aaattaaacc tgagcacagg
    15841 gagtcccagt gcctggagcg ccttcatcgc atggtagacc actttgttgt gcatcgtgcc
    15901 accgcccagc ggatgcggat ggcagaccag agccgcatac tgtgcgtcag gagcgcctgc
    15961 attcagaagg gcctccagtt ttcccgcagg cccgcggagg tcatcaacgg tccggagcgt
    16021 cgctgtgccg gaatgaatcg ttgtcattgt ccaacaacat tgtattctga gactcgatga
    16081 cgtttgatcc agtccaattc acccggcggc tcattgacat tgagtccatc acgtataacg
    16141 aaggcgcggc aggtgcgttt ctggccgatt ttctggcgca gcgcggcttt tccatcgaga
    16201 agatgccggt ggaatcgccc gagcgtttta acgtttatgc ctgtgtgaaa gggcaaacgc
    16261 cggacatcgt gctttcgacc cacatggaca ccgtgccgcc gttcatcccc agcagtgaag
    16321 atgatgagtt tatctatgga cgcggcgcct gcgatgcaaa gggcataatc gccgctcaga
    16381 ttggagctgc tgaacggctg cgcgattccg gcgtcaaggt gggcctgctg tttcttgttg
    16441 gtgaagagcg cgactcagcg ggagcaaagg tggcgaatac gcaaccgaag ggcagccgtt
    16501 ttctgatcaa tggcgagcct acggacaatc gtctggcgat tgcctcaaaa ggggccttgc
    16561 gcgctgcggt ccgcgcaaag gggaagatgg cgcattcggc ttaccctgag ctgggtgaga
    16621 gcgctgtcca taaacttgtt gaagcactga accgcgttct tgaaatcagg ttgccggtca
    16681 ctgaggatat cggcccgagc acgttgaata tcgggctgct ggaaggcggc cgcgcgccta
    16741 atgtgattgc cgatcatgcc gaagcacagg tcctggtgcg gctggtgggg tcctcggaag
    16801 aaacgcgtaa ggcactcgaa gaggcggttg ctggcctggc agaggttgag ttcacgctgg
    16861 agattccttt catgcgtatg cgtgccctcg atggttttcc gactatggtt gcggccttta
    16921 ccacggacat tcctgcgctc acaaactggg gtgagcctgt tttgctcgga ccgggttcta
    16981 tccatgtagc gcatactccg catgagaagc tcgcgaaacg cgagctactg gaagccgtgg
    17041 acctctatat cgaagttgcg aagcgcctgg tggggtaggt tccggaatga tgacgcgcgc
    17101 agcacgatgg cgcaaagtaa cccgggcggt tttgctggga tggaccgttg ctttccttgc
    17161 gacgtctcca ttccagatct gtgaagtatg gcgcaacagc ggccgcagcc ttacgctgct
    17221 ggtctctgct ctggggtatg gttttgccgt ctggttccta ctcacactgg ctggcgcgct
    17281 gcttacctgg atttgcatcg caacgcccat cgcaattttt atccacccgc agaggctcct
    17341 ccgccggcgc ggtcttgtga tgggcctcag catcgcggct gcattgctgg tggtggctta
    17401 tcaattgcac atctggacac attggtatca cgacggcgtt gggttgatga acttcgccat
    17461 atacgcgctg tttgctacgg tcttttccgg cactgcggcg tatgcgtatc tgcgttcgct
    17521 tgaaagctcc tggacccgct cgcaggcatc cgatcattca taacgcgata cactgcggaa
    17581 aatggtccaa cttaacgcac tcgaacatgc cgcatcgtct tatctgcgct cagccatgca
    17641 tcagccagtg caatggcatg agtggtcgcc ggaggccttc gcgctcgcag ccaaagaaga
    17701 caagcctata ctgctggaca tcggcgcggt gtggtgccac tggtgtcacg tgatggaccg
    17761 cgaatcctat gaagatccca cgttggcgca ggtcattaac gaacactatg tcgccatcaa
    17821 agtagaccgc gacgagcgtc ctgatgtgga cagccgctat caggccgccg ttcaggccat
    17881 gtccggccag ggtggatggc cgcttactgc gattcttact ccggacggca agccgttctt
    17941 tggggggacg tactttccac gcgacgaccg ttacggacgg cccgggttcg aacgcgtatt
    18001 gcgcacactg gctgatgtct ggaagatgaa acgcgccgat gcattggaaa cagcgcagag
    18061 cgtcattgct gccattgagc acggcgagac tttctccggg cgtgcatcca gcctcacgcc
    18121 tgagcttata gacaagctgg ttgtgagcag cgtgagtcag ttcgatccgc gttacggtgg
    18181 attcggctcg cagcccaagt tcccgcatcc ggctgcgatg gacctgcttc tcgacgtggc
    18241 tgcgcgcgga aacgaaggcg caaaaaaggt cgcaaccttt acgctggaaa aaatggctgc
    18301 cggcggcatt tatgaccagc tcgccggagg cttccatcgc tattccgtgg atgaacgctg
    18361 ggtcgttccg cactttgaga agatgctgta cgacaactct gaactgctga agaattatgt
    18421 tcacgccttc cagagtttcg tgcagccaga gttcgcgcgc gtcgcaaagg acatcatgcg
    18481 ctggatggat gagtggctca ctgaccgcga acggggcggc ttttatgcat cgcaggacgc
    18541 cgacatctcg ctcgacgatg acggcgacta ctttacctgg acgcgcaaag aagcagccgc
    18601 tgtactgagc agtgatgagc tggaagtcgc cgggttgtat tacgacattg gcgagatggg
    18661 cgacatgcac cacaatcctg aaaagaacgt gctgcatgtg gagtactcgc ttgaacaggt
    18721 cgcgcagaag ctttcgcgcg atgtaaagga tgtagagcgc gatcttgttt ccgcgaaaaa
    18781 gaagctgcac gcagcgcgat tgcagcgtcc tgcaccgttc attgataaga ccatctatac
    18841 cggatggaat gcgctggcgg ttacagctta tctggaagca gcgcacgtgc tgcatctgcc
    18901 tgcggcgcga gtattcgcgc tcaaaaccct ggatcgaatc ctggacgagg gctggaatac
    18961 gcaggagggc ctgacccacg tcattgctta cgccgatgga gttgcagcaa aacagcgtgt
    19021 cgcaggtgtc ctcgatgact atgcatttct catccatgcc tgcgtggatg cgtggcaggc
    19081 cacgggcgtt ttgcgttatt acaccgccgc gatggaaatt gccggcgtca tggtcacacg
    19141 tttttatgac gcaactggcg gcggattctt tgatacagaa catagcaagg acgaagcaat
    19201 cggcgctctc tcagcgcgtc gcaagccctt gcaggacacg ccaacccctg ccggaaatcc
    19261 tgcggcggca gctgctcttc tacgtcttga agccctcagc ggacgcagcg atttccgcga
    19321 aaaggccgaa gacacgctgg aacactttgg aggaatcgtc gaacatttcg gcctctttgc
    19381 cgggacatac ggccttgcac ttgaacggct cctgttgccg cctgtgcagg tggtcgtaat
    19441 tggcaatgac gagcaggcgc agaacctttc cgcgatggcc acagcacgct atgcggtgaa
    19501 caaatccatc attgcgctgc ggcaggagca ggtgagggcg gaaaacctgc ctccagtgct
    19561 ggctgagacc ctgcccgaga ttccaaatct gcaatctggc gggagctttg ccgtcgtctg
    19621 caagggaaat gcctgtcagc cgccaaccaa tgatgcggaa gagctgcttc gagaattgag
    19681 tgaagcctga actcgaagac atgcggcact ctgtgatgaa atctgtcttg tgatcgtgcg
    19741 cttggtaaga aaggcaattc tttgctgtct gcttgttccg gtttctctgg gcgcgatggc
    19801 ttatgctgag tcatccgttg aacaggcgct cagacaggca gtacacggaa caccggcagt
    19861 cgctgttgtc ctcgatgtaa aaaatggcag gacaattgcc gctgtcgggg ccaatgaagg
    19921 ggcaatgctg cgcactgctc ccggctcaac actgaagccg atgtttctgt cctatgccct
    19981 tggacacaga ttcattcagc cgcagacaac agccttttgc catcggggac tgcgtattgc
    20041 cgggcgcaat ctggactgca cacatcctca gagcgagaag gtattcaatg cagagctggc
    20101 gctggcgtat tcctgcaaca actattttgc agagatggcc aggcgcttca ctccagagca
    20161 ggctgaagat gcattgcgcg agtttggcct tacgggtcag atcgagagcg gggcttctat
    20221 agagaaattg cagttttttg ttttgggact ggaaggtatt cgtgtatcac cagacactct
    20281 ggcgcgcgca tatcgtaaac ttgcggcgca atggaatgcc ggtttgcccg atgcagtaaa
    20341 gcgcgggctt gaggattcag tgcaatacgg catggcgcac aatgcgtatg tgccaggcgt
    20401 gagggttgca ggaaagaccg gaacggcgag cgaaacggga cagccgtgga cgcatggatg
    20461 gttcgctggc attgttccgg cagaccatcc aaagatcgtg atcgtcgtct atctgccgcg
    20521 cgggaatggg gccgatgcag cgcatcttgc ccagcacttc ttttctgctt ataaggacgc
    20581 tccgcgatga aggccgttct gatttatatt ttgctgatcg ccggcggggc ctgcgcgcaa
    20641 aacacgaatg ggcctgccgc gcttactgtg cggctgtttt cgtcgcaccg ggcggatgca
    20701 gtcacatttg ttccgttgag tataaatagc aaaataaaaa cctgcgcgaa atgcgcagac
    20761 aggccgctgc atgggccagt cactctgaag aatactcaga tgaagccggg tgtgagggtt
    20821 gtccttcggg gagcatttcg cgcaagcatt gacaatgctg aacaaagcga aacagccgct
    20881 ggggaatgga cggtcactcc agcgaagaac ggcctgcggg tgctgctaac taccacagcg
    20941 gaacattatg tgatggctgc gctcagtgga gaagctgctc cggatgaacc actggaatcg
    21001 ctcaaagcga tggcagttgc ggcccgcaca ttcgcgattg tggaccagca ccgtcacgag
    21061 gcggaaggtt ttgatctgtg tgacagcacg cattgtcagg ctttgcgttt tggccatgtc
    21121 cgtccggaga ttgaacaggc agtgcgcgcg accgcagggg agacgctgtg gtttggcgcg
    21181 caccgggcaa acgtatatat gacacagcat tgtggtggcg aaacagaaga tgcgtcgagc
    21241 gtgtggcctg cggtacgtgc accatatctg cgcgctcatg cagatccgta ttgtctgcgg
    21301 cgctcccagg caaaatggta cgccgatatt caagtgccgc aactggcccg catcctgtat
    21361 ggagagggat ggaagatacc tgcgcagatt gattccgtgc aggtggtgaa gcggacagca
    21421 gcaggaagag cacagatgtt gcaaatcaca ggtcagggcg agagcacgcg catatcggcc
    21481 agcagcttcc ggtttgcgat aaaccgctcc cttggctgga accaattacg cagcgactgg
    21541 tatgaaatca cacgcaacaa taacatgctg cattttgaag gcaagggcta tggtcatggc
    21601 gttggccttt gtcaggcagg cgcatttgaa atggcgaagg aagggcacaa ctatcgcgag
    21661 attctcggct tttattttcc cggaacccag gtacgggtga cagcgaagga ccaaggatgg
    21721 aagcaggttg gtggtcaggg ctggaccttg atggcgctgg gcggcgtaga cattgtgaat
    21781 gccggaaatg ctgcatggac gaaggcgcag gcggtgtttt caccgaaatc ttcagtccat
    21841 cctgttgtgt atctgatgcc ctccactgag ttatttcgcc agactacaga tgagccgggg
    21901 tggatgctgg cttccactcg cggaacggat gtttatctgc agcctgtcgc cattatgcgg
    21961 gcgcacaggg gagcatcttc tgtgctgttg catgagtttc ttcatgttct cgttgaacag
    22021 gaggccacag caaaggcccc tttgtggctg cgtgagggac tggtggaggc actggctgga
    22081 gatattcacg cgcgagaggt attggcgccg aaggcagcaa tgctggatgc cggattggcg
    22141 catcccgtaa gtttggcaga gtcgcagagg gcgcattttg ctgcgggcga gctggtgcaa
    22201 aagctcatac agcaatatgg cctaaataca gtgcgaggct ggctgcaggg cggagttcct
    22261 gccgcggttg tagctgaaaa ttagctgcta aaatctatat atgcgggtgc gtaccatctt
    22321 cgcggcggtc atggttgttc tgctgatagc gggaatgacg acctcggcca tgtgtgagat
    22381 gacgtgcctt ccgaaggccc agaccgcggc ctgttgtccg caccatgcgc agcagatggt
    22441 gatgcattgt cagcagcatt cagagacagc ttcattcgtg aatgctcata cttgtgaaca
    22501 tccgcaggac agtgccgctg ttgcagcctg tatcgtagcc gcgccatctg ttatatcggg
    22561 cgtcatcatt ggtacaaacc tccagcgtgc gcctgttttt tacgcacatt cgtcttcaac
    22621 tcccattctt aaaccattgc atcctcctct tcgcatctga ttgcctccac tcttcgtgtc
    22681 tgtacggcgg aaatcttagt gcgcactttt ctattcatga tgaggagttc ttatgaccag
    22741 agtttttgtt cttttgctgg ttttgtgcgg tgttgcggct gcgcaggaca tgtctgaaat
    22801 gatggaccgc ggcaatcaca gctttacgca acagattgtg catcatgcta cgtcaggcac
    22861 cagcgccgag ccgaattcta ccccagtgcc catgtggatg aggatgcagg gtgactggat
    22921 gctgatgttc cacgccaatg catttgtgat ggatgagcag cagacaagtc cgcggggtgg
    22981 ggacaagttt ttctcaacga actggtttat gcctatggcg caacgccaga tggggccagg
    23041 atatcttaca ttgcgcggaa tgttcagcct ggaaccggcg acgattacac agcggcgcta
    23101 tccgctgctt tttcagcagg gcgaaacagc ttatggcaag gcgattgtag acggtcagca
    23161 tccgcataat tttctgatgg agctggcggc catttacgac tggaagatca gcgaaaaaac
    23221 tttgcttacg ttttatgctg cgcctgtagg tgatccggcg attggaccta cggcgtatcc
    23281 gcaccgcgct tctgcatttg aaaatcctgt agggacatta ggtcatcatc aggaagattc
    23341 gacgcacatt gctgatgatg tggtgacggc cggatttacg catggtctgg cgcgcatcga
    23401 ggccagcgga tttcatgggc gagagccaaa tgaacatcgc tggcaggtgc agcaaggggc
    23461 gatggattca tggtctacgc gtctgacctt gcaacccgga caaaactgga gcgggcaata
    23521 ctcgtatggg cgcattcaca gcccggaggc gcttttcccg tctgaagacc aggagcgcat
    23581 gacggcatcg gtgatgtaca accgtccgct gaagaatggc aactgggcca gcaccttggc
    23641 gtggggcagg acgcgcgcac tgacagacaa cgtaattcag aacagctatc tgctcgagtc
    23701 cacgctgcaa tttgcaaaga caaattacgt atggacgcgc atggaaaatg tagaccggac
    23761 cactgagctt ttgcatgacg caggcccaga ggagccggcg ggacgcgttc aggcttatac
    23821 gtttggttat gaccgtgatt tcaggacatg gccgcactgg gcgactgcat taggggcgca
    23881 ggtgtcggtg tacagtcctg gatcccgtct tcaacctgtc tacggaactg acccgatggg
    23941 ggtagttgtg tttctgcgtc tgcggcccag cgggaaatga agccctcatg tggactgcgg
    24001 catcgaaata acagaaccca ttgatggcaa ttgcaaatca acagaaaaaa acatccataa
    24061 aagatttttc ccatgcgtgg cgcgcgctgc gccaccgcaa tttccgcctg ttctttggcg
    24121 gtcagagcat ttcgctcatt ggcacctgga tgacgcgcat tgcgacgtcg tggctggtgt
    24181 accggctgac gaaatcgtct ctgctgctgg gcacggtcag cttcgcgggg cagattccta
    24241 cctttctgct tgcgcctttt gcaggtgttc tggtggaccg gatggaccgc cgtaaagtgc
    24301 tggtctggac gcaggtgctg gcgatgatac agtctctggc gctggctggg cttacgctgg
    24361 cacacatgat caatattcgc gaagtgctgg tgctgagtgt ctttcaggga atgattaacg
    24421 cgtttgatat gccaggccgc caggccttta tggttcagat ggtggaagac cgcgcggacc
    24481 tttccaacgc aattgctatc aactcgtcca tggtgaatac ggcgcgcctg gtcgggcctt
    24541 cgctggctgg gttggtcatc gcggctacaa acgaaggctg gtgttttctg atagatggca
    24601 tcagctatat cgcggtgatt gcttcgctgc tgattatgcg catgcgcgca ggccaggaga
    24661 agcgagcggc tgcttcgatg attgagcaat taaaagaagg ctgggcgtat gtgtccagct
    24721 tcgtgcccat tcgcatgatt ttgcttctgt ttgctcttat cagcctgatg gggatgcctt
    24781 ttgttgttct tatgcccgtc tttgcagcgc aggtactgca tggagggccg cacacgctgg
    24841 gctttctgat gggcgcgctc ggcgttggag ccttaatttc tgcgctctcg cttgtagtgc
    24901 gcaaatctgt acgtggtctg tcgaagatga ttccgattgc tgctgcggtc tttggcgcgg
    24961 ggttgatcgc cttcggcttt tccacgaacc aatggctctc tctgttgctg atgctgttca
    25021 ccggtttcgg catgatgcag ggtatgacag ccagcaacac catcattcag actctcgtgc
    25081 ccgaagacaa gcgtggccgc gtgatgagct actacacgat tgcgtttgtt ggcatggctc
    25141 ctttcggaag cctgcttgct ggaggactgg cccatgcaat cggagcacag gagacggtcg
    25201 ttatcagcgg cattgcctgc atggcagggg cgctgtggtt ctggtcgcat ctgaaggacg
    25261 tccggaaaga gatgcggcca atttatgaac agcttggcat tcttcaaccg aaggtgccca
    25321 ttgtggtgga agaggaagtg agccgttgag atcgcatctt tccaaaacta ctccacacat
    25381 aatgaggcac tcaatcaccc atattcttct aaccgcgcag actggctggg ggtggattca
    25441 cggctgaaag cccggcgaat ttgtcctccg gcaactcgag acttgcggcg gcaatgtttt
    25501 cttcgaggtg cgcaaccgac gaagttcccg gaatcgggac catgacgctg gagcgtttca
    25561 gcaaccaggc caaggcaacc tgccgtgtgc ttgctccgag ttcggcggca actttctcaa
    25621 tggcttcatg cgctttgcgg ccttgtccta atggcgccca gggaaggaaa gcgatgccat
    25681 tccgttcgca gaagtcgagg acgtaatccc actcccggtc ggcaaagctg tagcggttct
    25741 gtacagacac gatgggcaca atcttccgcg cgcgttcgat atgttccagc gtgacgttgg
    25801 agagcgcaac gtggcggact ttaccctgtt ctcgcaggcg ggcgagtgcc tccattgagg
    25861 cgtcaaaaga aactgcggga tcaggcacat gaagctggta cacatctata cgatccagac
    25921 gaaggcgctt cagactgcct tcagcagctt cgatgaggtg cttcgggctg gcgttgtgga
    25981 tccactggcc tggaccgacc cggagccatc cgcctttggt ggcgatgacc agacctttgg
    26041 gatagggata cagtgcttca gcaatcaatt cttcagagac attggggccg taagagtcgg
    26101 ctgtatctat gaagttgacg tccagctcaa ccgcgcggcg aagcgtcgcc agggcggctg
    26161 ctttgtccgc tggaggtccc catatcccgt gtccggtaat gcgcatggcg ccatagccca
    26221 tgcgatggat ggtgagatcg cctcctaaag taagcgtccc ggcagcggca ccagagatgg
    26281 ttttggtcgc agtcatcgga tgtcctcctc ggttacattc cgccttggcc tatgtccatg
    26341 cttttggcga tggggttacg ctcgtcaata tacttttcga tgacttcttc cagaattcct
    26401 tttgcttcaa gaataaattc aactgcatcg cgtccggcgc catgtccgcc aaaatttggc
    26461 gtgatgtagt gggccgcatc ctttacctgc ttgcgggcat tggccacggc aatcgccagc
    26521 ccgacttcac gcatgacggg caggtcaatt acatcgtcac ccacgtaagc aatttcttcg
    26581 agcgtgacgg attcctttgc catgatttcg cgtacggcct gcatcttaaa tgcctgtccc
    26641 atatagacgt attccagccg caggtcgcgg gcacgaaggg caactgtttc agagatgcgc
    26701 ttggtgataa tgccgcattt cattccgccc aggcgggcga gcgaaacccc ggagccatcg
    26761 tgcgcgttga agcccttggc ttcgaccata ttggcggagt ggatggcgta tccacctttg
    26821 ctttccattt cctgagcatg ggcagcagtt gtctggctgg gtgctggagc agggaagatc
    26881 cagataccgc cgtctgtgag cactccgtct acgtcaaaaa ggataatttt gatacgtctg
    26941 gcgcggtctt cagcagagag aatttgatct gacatattcg cattgtaatc aggggacaga
    27001 gcagctaact tttgaacctc atatttgttg tcgggcccgc cggcagatgc ttttcaaacg
    27061 gaacgcgcac gagttggctg cgcgtccgca ccccgacttt ctgaagtaaa tcctgaaatc
    27121 aacagaagca ttccggaaat aaggctttcg ccaaaataga acagagcgta caacgcattt
    27181 cattgttccg ggactctgat aaagagttat gcggagggta ctttgaaaat cattggcata
    27241 cttctgattg ctcttggagt gatcgctctg atttatggcg gcattaccta cacaacccac
    27301 aaaaaagtga tagacatggg cccgattcag gctgaaaaga aagaaacgca caacgtcccg
    27361 ctgccacccg tgcttggcgt tatcgctatc gttggtggag gcgcgctgct ggtgttcggc
    27421 gccaaagcca ggtaaggccg tcttcctgcc atgcccgttt tctggtcccg gaactttgct
    27481 tagggcaaac tctgtgaaat cacacaggca ggcggcggtt tgtccggcac ccttcctcca
    27541 aaccatttac actataggtt gaggaaaccc tgctttgatc ggtagtgctc ttacaaaagt
    27601 ctttgggacc agcaatgaac gcgcggtaaa gcgcctgatg cctgttgtcc aggccattaa
    27661 cgccatggag ccggacgtgc agcggctctc agatggcgag cttcgcgcca aaacagacga
    27721 gttcaaggtg cgcattgcca aggccctgga aggcatcgaa gatgccgagg aacgaaaggc
    27781 cgctgagaag cgggcacttg atgaagtcct gcccgaagct tttgctgtag tgcgcgaagc
    27841 aggccgccgc gttctgaata tgcgccactt cgatgtgcag ctcatcggcg gaatggtgct
    27901 gcaccagggc aagatcgcag aaatgaagac cggcgaaggc aagacgctgg ttgccacgct
    27961 gccttgctat ctgaatgccc tcgccggaca tggcgtccac gttgtcacgg taaacgacta
    28021 cctggccaag cgcgacgccg agtggatggg caaaatctat gagttccttg gcctcaccgt
    28081 cggcgtcatc gtgcatgatc tcgatgacaa tcagcgccgg caggcctact ccgccgatat
    28141 cacctacggc acgaacaacg agtttggctt tgactatctg cgcgacaaca tgaagttcga
    28201 gttggccgat tgcgtgcagc gcggccatta tttcgccatc gtggacgaag tggactcgat
    28261 tctgattgac gaggcacgta cgccgctcat catctccggc cctactgacc agacgaccga
    28321 caagtatgtc cgcgtagacc gcattatccc tcagcttgtg caaggcgaag aaatcgagca
    28381 gggcgtagat aaggaaaaaa tcctcaccgg cgactacgta gtggacgaga agcacaaaac
    28441 catcagcgtg accgacgagg gctgggaaaa aatcgagaag ctgctcggca tcggcaatat
    28501 tgccgacccg gaaaactggg aatacaagca ccacgtcgag acagccatca aggcgcactc
    28561 gctttacaag cgcgacgtgc agtacgtagt gaaggatggt ccgcagggca aggaagtcat
    28621 catcgtagac gagtttacgg gccgccttat gcctggacgc cgctggtctg acggtctgca
    28681 tcaggccatc gaagccaagg aaggggtgaa catccgccgc gaagaccaga cgctggcgac
    28741 catcaccttc cagaactatt ttcgcctcta ccagaagctc gccggcatga ccggaacggc
    28801 ggaaacagaa gcggccgagt ttgacaaaat ctacaagctg gaaatcgtcg ttattccaac
    28861 gaacaagccg ctcctgcgca ttgagaatcc ggacgtggtt taccgcactg caaaagaaaa
    28921 gtactttgcc gtggcggacg aaatcgcgcg cctgcatgaa aaaggccagc ccgtgctggt
    28981 gggcaccact tcgattgaaa agtcagaact gctctcgcag atcctccaac gcaaaggtgt
    29041 aaagcatgtc gtcctgaatg cgaaatatca cgaacgtgaa gccgagatcg tggcgcaggc
    29101 tggacggctg ggcatggtca cgatcgccac gaacatggca ggccgtggca cggacatttt
    29161 gctcggcggc aatgcggaat ttgtagccaa acaggagctg gtgaaaaaag gcctggcccg
    29221 cgccatcagc gtggcagaag gcgcgctgaa ccccatggcc gctcccggta tgctgcgctt
    29281 ctactatcag ggccaggagt tcgagacttc ccaggaaaac tgggacaaga cttacgcggt
    29341 ccacgctgca aaggcccagg aagaacacga tgccgtcgtc gccgcgggcg gtctgcacat
    29401 tatcggcacc gagcggcacg agtcgcgacg cattgacaac cagcttcgcg gacgcgccgg
    29461 acgtcagggt gacccggggg catcccgctt ctatctctcg ctcgaagatg acctgatgcg
    29521 tatcttcgcc aaagagtggg tagggacgct tctgcagcgg ctcggcatgg aagaaggggt
    29581 gccgattgag tcgcgcatga tctccaaacg tatcgaggcg gcacagaagg ccgtcgaagc
    29641 acagaacttt gaggcccgca agcacctcct cgaatacgac gacgtgatga acaagcagcg
    29701 cgaggccgtc tatgggctgc gccatcagtt gcttgaggga ctagaccaga aagagctcat
    29761 tattgaggac tacgttgcca ccatcctcag taatttactg gatgagcaca cgccggaaaa
    29821 gatccatccc gagcagtggg acctgaaggg tctgaaagaa aagctcatca gccacttcgg
    29881 cttcgatcca gaggccgagg gtatcaatct cggcgagatg acgcgtcacg agatcggcga
    29941 gaccatcttc gagcgcctga aagagcgcta cgaggccaaa gagcaaatca tcggcgacaa
    30001 gcagatgcgt ttccacgagc gcatgattat gctcagcgtg ctggatggtc tgtggaagga
    30061 ccatctctac aacatggacc acctgaagga aggcatcgga ctgcgcggct acggccagca
    30121 ggacccgctg gtggcctata agaaggagtc cttcgatatg ttcgagggga tgatggcgcg
    30181 tttccaggaa gacaccgtgc ggttcctctt ccatatgcag atcgtcggcc cggatggccg
    30241 cccgttgcag gtgccatcgc gtccgcgtcc gtctgttccg gcagcgcctc cggtggcctc
    30301 ggcagaagca gttccgcaac acgtatcttc agcggtcgcg gtagcagagc ctccggcagt
    30361 gcccattccg gtgcgtcagc cttctactac gattgacgag atcgagcgcg aattccaacg
    30421 caaaaagcag cgcgaactcg aacatgcccg catggccgga actggcggcg atagtgccgc
    30481 tcccgtacaa cgcagaacag gcgacaagat cggacgcaat gacccatgcc cctgtggttc
    30541 cggtaaaaaa tacaagaaat gccacggagc aggcgagggt taaacctggt ccgcttctga
    30601 gggctatggc attttcacag tgccgtgccg cccggtctct gttggcggcg acgacttagt
    30661 tttctggcat cgttcagact gtctgtgaaa atgctatagc tgaaaaatga tcaactcgcc
    30721 agcacgcgat caaaaatcgc gtccacattt ctaagctggc ggcttaaatc aaaagttttt
    30781 tcgagctttt ccggggcaag cttcgccgtt atctccgggt cggttttcac cagatcacga
    30841 aagaccaggt cttctttcca agcgcgcatg gcgtgcttct gtaccaggcg gtaggcgtct
    30901 tcgcgcagca ttccggcctc ggcaagatcg agcagcaact gtccgctgaa gatcaggccg
    30961 ccggtacttt caagattttt caacatgcgc tgcggataaa ccagcaggtt ctcgagcagg
    31021 ttcgtggttt tggcaagcag atagtcggtc aggatggttg aatcggggaa gatgacgcgc
    31081 tcgactgatg aatgcgagat gtcgcgctca tgccaaagcg ctacattttc atacgcagcc
    31141 tgggcgttgg agcgtacaac acgggcaagc ccgctgattt gctcagaagt aatcggattg
    31201 cgcttgtgag gcatcgccga cgagcct
//
LOCUS       DASRBV010000293         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_687140, whole genome shotgun sequence.
ACCESSION   DASRBV010000293 DASRBV010000000
VERSION     DASRBV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687140"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFA02_10930"
     CDS             complement(<1..515)
                     /locus_tag="VFA02_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZU10403.1"
                     /translation="MWKPNQTGNTSPTPSEPVRPAAPATPSYESNVRSTPAAQSASAD
                     QATIGKSLIIKGEVTGSESLYIDGKVEGSINLPGNRVTVGRNGQVTANISAREIVVLG
                     KVRGNVNASDRVDIRSEGSLSGDVVAQRISIEDGAFFKGGIDIRKPGNEKAEVKPNSG
                     ATQTETAKAVTV"
     gene            773..3301
                     /locus_tag="VFA02_10935"
     CDS             773..3301
                     /locus_tag="VFA02_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HZU10404.1"
                     /translation="MTDRELLRQIERSAGQKAGYKQLVREFGLGGGRDRRLLLEQLAR
                     LTARGELTKLDRDHWGIPRAPAQRDNLVAGRIDLHRDGYAFVRPQDRSRTGDEDIFIP
                     PNELSGAMQSDQVLVELLPPRADGRRLGRVLRVLTRKNPTVVGVFHYARGERAQGHLV
                     TPFDERMTQPILIPFGMELPQKKQTVRTPHRVLGEEAKQRSYDDLEGLIVDVEITEWP
                     TMSRAARGRVIEVLGAEDDFGVDVEIIIRKHHLPHVFPEKVLEEARSVAFLDPEVVAG
                     RRDFRHLPIVTIDGETARDFDDAVLVRENGDGTFELQVHIADVAHYVREGTALDLEAR
                     LRGNSVYFPDRAIPMLPQELSTDICSLRPDEDRLVLSCIMQIDARGDVLGYEVTEGVI
                     RSARRMTYTQVHAILEGDEDTREQFREFVPEFERMHQLAQVLNKKRARRGSIDFDLPE
                     PIIEFDEFGAMKSVSRSERNWAHRLIEEFMLAANECIASWIEQQGVASIYRIHEKPEP
                     KRVVEFEETAAAFGYSLGVGNLPVRKFQMKSERREQHGRGRNPRQHEIPEDIHVSPRM
                     YQRLTAKIAGKPEERILSYLMLRSLKQARYSERNEGHFALAAPCYTHFTSPIRRYPDL
                     IVHRITKALLAEGVSGAGKLLPGEPHAATDRKSPRQNPAQERAARGGLDGQSIANVAQ
                     ETSETERRAADAERELVEWKKVKFMQDRVGEDFSAMVLTPTKYGLFVELDDLFIEGLV
                     PIQSLGQMDGDYYTYRENTREIIGQRWGRKFTFGQNVRVVLDRVDARERRLQFSVLDE
                     QTVPTTKGGDRTTEKKPRRQKIQKRSTRQNKKAKKSSGKNKRKK"
     gene            3363..3539
                     /locus_tag="VFA02_10940"
     CDS             3363..3539
                     /locus_tag="VFA02_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10405.1"
                     /translation="MKLLAYLTEMFISTFGITRPRPDQQRQANLVIGGSLLMFLLLVF
                     SVFGFLIYQIVGSK"
BASE COUNT          792 a         1062 c         1046 g          781 t
ORIGIN      
        1 accgtaacgg ccttggctgt ttccgtttgc gtagcgccag agttcggctt gacttcggcc
       61 ttctcatttc cgggcttgcg gatatcaatg ccgcccttaa agaaggcgcc atcttcaatc
      121 gaaatgcgct gtgctacgac atcgcccgag agcgagcctt cgctgcggat gtccactcgg
      181 tcgctggcgt tgacgttgcc gcgcactttg ccgaggacga caatttcacg ggccgagatg
      241 ttcgcggtca cctgtccatt gcgccccacg gtcacacgat tgccgggaag attgatggac
      301 ccttctactt tgccatcaat gtacagcgac tcggaaccag tgacttcgcc cttgataatc
      361 aggcttttgc caatcgtggc ctggtcggcg gaggcagatt gcgctgcggg cgtgctgcgc
      421 acgtttgatt catagctggg ggttgccgga gcggccgggc gaactggctc acttggggtt
      481 ggggaagtat ttccggtctg gtttggtttc cacatctgac taaagtttcc tttcttctcc
      541 tctgaagctc actccgggca tccctcgccc atttttgttc agggctcccc ggaggtattg
      601 aacgtcgaac cttgtcccat tcgaggaatc ctcgaaatcc ccgaatccgc gcagtcggcg
      661 ctgctgtctg catcttaaac agggttgggt aaaaaatgca ccccattctc aatctttttc
      721 tcgaaaaatg tgctgagaat gtcctgaggt caaaaggcgc tactctcatt gtatgaccga
      781 ccgcgaactc ctgcgccaga tcgaacgctc tgctggccaa aaggccggat acaaacagct
      841 tgtgcgcgag ttcggcctgg gtggagggcg cgatcgccgg ctgctgctcg aacaactggc
      901 gcggctgact gcgcgcggcg agctgactaa actcgaccgc gaccactggg gcattcctcg
      961 cgctccagcg cagcgcgaca acctcgtcgc gggccgcatt gatttgcacc gcgatggata
     1021 cgcctttgtc cgcccgcagg accgcagccg cacgggcgat gaggacatct tcatccctcc
     1081 caatgaattg agcggcgcca tgcagagcga ccaggtgctg gtggaactgc ttcctccccg
     1141 cgccgatgga cgcaggctgg gacgcgttct gcgcgtgctc acacgaaaga acccgactgt
     1201 cgtcggcgtc ttccactatg cgcgcggtga gcgggcacag ggacatctcg ttacgccttt
     1261 cgatgagcgc atgactcagc ccatcctcat tccttttggg atggaactac ctcagaaaaa
     1321 acagacggtg cgcacaccgc atcgcgttct gggcgaagaa gcaaagcagc gcagctacga
     1381 tgatctggaa ggcctgatcg tagatgttga gatcaccgaa tggcccacga tgtcgcgcgc
     1441 ggcccgcggg cgcgtcattg aagtgctggg cgcggaagac gatttcggtg tggatgtcga
     1501 gattatcatc cgcaaacatc atctgccgca tgtctttccg gaaaaggtgc tcgaagaagc
     1561 gcgcagcgtt gcttttcttg atccggaagt ggtggccggg cggcgcgact ttcgacatct
     1621 gcctatcgtg accattgacg gcgagacagc ccgcgatttc gatgatgccg tcctggtgcg
     1681 tgaaaacggc gacggcacct ttgagctaca ggtgcatatt gccgacgtgg cccattatgt
     1741 ccgcgaaggg acggcgctcg acctcgaagc gcgtctgcgc ggtaactccg tgtatttccc
     1801 tgaccgcgcc attcccatgc ttccgcagga gctttccact gatatttgca gcctgcgtcc
     1861 ggatgaagac cgcctcgttc tctcctgcat catgcagatt gacgcgcgcg gcgatgtgct
     1921 tggctatgag gttaccgaag gcgtcattcg ctcagcgcga cgcatgacct acacccaggt
     1981 ccatgccatt ctcgaaggcg acgaggacac acgcgagcag ttccgcgagt ttgtaccgga
     2041 gttcgagcgt atgcatcagc tcgcgcaagt cctcaacaaa aagcgcgcgc ggcgcggctc
     2101 gattgacttc gaccttccgg agccgatcat tgaattcgac gagttcggcg cgatgaagag
     2161 cgtctcgcgc tcagagcgca actgggcgca ccgcctgatt gaagagttca tgctcgcggc
     2221 caacgagtgc atcgcctcat ggatcgagca gcagggcgtc gcttccatct atcgcatcca
     2281 cgaaaagccg gaaccgaagc gtgtggtcga atttgaagaa acagccgcgg cctttggcta
     2341 ttcgctggga gtaggaaacc tgcccgtgcg caaatttcag atgaagtccg agcgccgcga
     2401 gcagcatggg cgcgggcgca accctcgtca gcatgaaata ccggaagaca tccacgtttc
     2461 gccgcgcatg tatcagcggt tgacggcaaa gatcgcaggc aagccagagg agcgcatcct
     2521 ctcctatctg atgctgcgct cgctcaaaca ggcccgctac agcgaaagga atgaaggcca
     2581 tttcgcgctg gctgcgccct gctacacgca tttcacatcg cccatccgcc gctatccgga
     2641 tttgattgtg catcgcatca cgaaggcgct gctggcggaa ggcgtaagcg gcgcgggaaa
     2701 gctcctcccg ggcgagccac atgctgcaac tgaccggaaa agtccgcggc agaatcccgc
     2761 acaggagcgt gctgcgcgtg ggggcctgga cggccagtcc attgcaaacg tagcgcagga
     2821 aaccagcgag accgagcgcc gcgctgctga tgccgagcgt gagctggtcg agtggaagaa
     2881 ggtcaaattc atgcaggacc gcgttggaga agacttcagc gccatggtcc tgactccaac
     2941 caaatacggc cttttcgtcg aattagacga tctcttcatc gaaggactcg tacccatcca
     3001 gagcctgggc cagatggatg gcgattacta cacctatcgc gagaacactc gcgaaatcat
     3061 tggccagcgc tggggccgta agtttacctt cggacagaat gtgcgcgtcg tgctggatcg
     3121 tgtggacgcg cgagaacgca gattgcagtt ctccgtactg gatgaacaga ccgtgcccac
     3181 tacaaagggc ggtgaccgga caacagaaaa gaaaccgcgc aggcaaaaga tacagaaacg
     3241 aagcacacgg cagaataaaa aagcaaaaaa gtcttccggc aaaaacaagc ggaagaaata
     3301 agtggccctc tgcatcgcat agcattacac ttccccataa acaaccttgc actggctttc
     3361 ctatgaaatt gcttgcctat ctcacggaaa tgttcatctc tacttttggg attacccgcc
     3421 ccagaccgga ccagcagcgt caggcaaatt tagtgattgg cgggtctttg ctgatgttcc
     3481 tgttgcttgt tttttcggtg ttcgggttcc tgatctacca gattgtaggc agcaaatagc
     3541 gggatttttc aaagcaggcc tgagtctgcc aaccttggta accaacacac aacctgtcct
     3601 gattacaaaa gttgttattg tgtttcccgg gttgtatatt tgtcgtacaa accaataaaa
     3661 tcgcggtttc cacgctactt a
//
LOCUS       DASRBV010000294         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_687272, whole genome shotgun sequence.
ACCESSION   DASRBV010000294 DASRBV010000000
VERSION     DASRBV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687272"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFA02_10945"
     CDS             <1..517
                     /locus_tag="VFA02_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZU10406.1"
                     /translation="IETVRLLRPDLNDVEELNLIEQIEIEDKEDIQVLKGVCRILDAL
                     PQKYWTIVTSATDRLARVRLKQANVPIPDRIITGDAVTYGKPGPEPYLKGAELLGQRP
                     EDCLVIEDSASGAKAGHAAGCKVLATLFSHTVEDLKDADWIIESLDDVQVRVLNSSVE
                     IEFQPLFQKQA"
     gene            complement(514..819)
                     /locus_tag="VFA02_10950"
     CDS             complement(514..819)
                     /locus_tag="VFA02_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZU10407.1"
                     /translation="MSRYVLGKDAELDLDEIWEYIASDSIDAADRWLNKLFDAFEVIA
                     RNPRIGHHRKDLTSLPVLFWAVENYLIIYRCNDGEVQIIALTQGSRDVPMFLLREGR"
     gene            complement(816..1082)
                     /locus_tag="VFA02_10955"
     CDS             complement(816..1082)
                     /locus_tag="VFA02_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HZU10408.1"
                     /translation="MNVSLTPALERFVSGKVASGRYNSASEVVREALRLLEEQDNQRA
                     TRLEAFHRELKRRITSLDQGEKIGPDAARTRFKKKSETRRKKSA"
     gene            complement(1133..1414)
                     /locus_tag="VFA02_10960"
     CDS             complement(1133..1414)
                     /locus_tag="VFA02_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10409.1"
                     /translation="MRVLLERALVIILIALLVLLAADWLVYRIRFARGSAFDTVKINA
                     FMAVPLKNGKEELDYVSSQQVTCVRSMFPWAGDRPCWWVRRHTEKRIAM"
     gene            1507..3759
                     /locus_tag="VFA02_10965"
     CDS             1507..3759
                     /locus_tag="VFA02_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZU10410.1"
                     /translation="MANHSSRPDRLWTVFVTLLVTLAGWSSTQSVPNGPNTNPSADFY
                     VATNGNDAWPGTLSQPFRTVDRARLAVQSLKSHVSGRTIRVLIRGGVYYLPSTWTFTA
                     QDSGSSNTPVLYANYPNETPVISGGQVLTGWTQNANGWWQTTLPSGTYFTQLWVNGSR
                     RYRPRTTPYGYLYISGEYSTTGSTTTVNELSYSTHATNGVPASMANLGDVELINFEAW
                     DVPHMRIASVDTTNKRIVTTASLPKQSFFTGFIPGHRFLLENVKEALRLPGQFYLDRP
                     SSVLTYIPEKGESLSTAIIVAPHLQQILRATNLSDVTFQGLTFAYSDWHPGTNGYLSG
                     QADNGTPAALSLTNSTGVVFEADTIEHTGAYGIEFEGTGIAGGASPYLAQFRDGLITD
                     TGGGGIRVGGPATCAGSYPHTDENVPQHIYIGNNLITGGGRVAVVGFGIMIGDAHNIL
                     VEHNEISDFYSVGVGVGFNWGYSCNFAHDDVVQYNYIHDLGQGITSDFGAVYYLSGVN
                     TGNKILNNVVHDMDQDPVGYGGWGLYLDAGSTGVLVENNLVYRTTDASLHVNSWASPP
                     PSPAPPPSIFKNNILAYGAMGMMDRHNDTSFLSIVYENNIFYYDKASIQYGYWYCEGK
                     PVCTNYFQFDNNLYFDKAVSGGEPAQPFFKTPYFPANGGQQPPITRLTFQQWQNEGED
                     KQSLFADPRFVDPTPGTDNYTLQSNSPAFSIGFVAFDPKQAGRLPSATLSAPVNAPAF
                     PLLTTPITDF"
     gene            complement(3771..5081)
                     /locus_tag="VFA02_10970"
     CDS             complement(3771..5081)
                     /locus_tag="VFA02_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZU10411.1"
                     /translation="MQPPVSGLVRWLYLDLNSYFASVEQQLRPELRDRPVAVVPVEAE
                     TTCCIAASYEAKAYGIRTGTVVKEARRLCPEIVFVLARHREYARFHHLVLKAVDRCVP
                     VDRVLSVDEIACRLAGSQCKPENALALAKKIKETIREEVGEYLRCSIGLAPNELLAKI
                     ATDMQKPNGLVLLDAGDLPDLLYPLKLRDIPGIGSRMEVRLRVQGIHSVRGLLALEAK
                     QMRAVWGSLYGERYWYWLRGYDFDGPASVPQSIGHQHVLSPELRTMQKAFAVAQKLLD
                     RATARLRGRDLWARGLSVYMSFSVGREKKIWECHTRMVESQNIFSALETLKKMWAGCP
                     HGTPTFIGVELYDLVPGSQHTAVFFPEEQRRQKLAQAMDAIHAQFGEKALYLGGTHAV
                     RNAAPVAIAFASIPESESFPESKPVKNTPVEWKSAEQKTKRPPA"
     gene            complement(5219..5935)
                     /locus_tag="VFA02_10975"
     CDS             complement(5219..5935)
                     /locus_tag="VFA02_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU10412.1"
                     /translation="MSSTLAVLFTYLLFGIPAAIIGLPWTLLTSDISMLYRWAMWIAR
                     TGIKAAGIRVKAEGVERLGPRQQYIFMSNHVSNLDPPILLPLLPGRTSVFLKRSLMKI
                     PVLGYAMRLGDFIPVDRDGRVETAKQSVEKGREVLASGLHVTTFVEGTRSRDGRLLPF
                     KKGPFYLAMETGAPVVPVSIWGTESMMKKGSIRITPGTAHVVFHPPLWPRDYAAREDL
                     MAAVRASIASSLPEWMVQTL"
     gene            complement(5939..>6203)
                     /locus_tag="VFA02_10980"
     CDS             complement(5939..>6203)
                     /locus_tag="VFA02_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZU10413.1"
                     /translation="ADHIVLSGVFKQESIPENERLHPETIVAALKQSGKSAALFANAD
                     AIVRSIVPQLRSGDVVAVLSNGGFDGIYEKLPRALEQRTFVRS"
BASE COUNT         1415 a         1772 c         1622 g         1394 t
ORIGIN      
        1 gatcgaaacg gtgcggctgt tgcgccccga cctgaatgac gttgaagagt tgaatctgat
       61 tgagcagatt gaaatcgaag acaaggaaga tattcaggtg ctcaagggag tgtgccgcat
      121 cctcgatgca ctgccgcaaa agtactggac gattgtcacc tcggccacgg accggctggc
      181 gcgtgttcgc ttgaagcagg ccaatgtgcc gatacccgac cgcatcatca cgggcgatgc
      241 agtcacgtat ggcaaacctg gccccgagcc gtatctgaaa ggcgcggaac ttcttggcca
      301 gaggccggaa gactgcctcg tgattgagga ttcagcctca ggcgcaaagg ccggccatgc
      361 cgccggatgc aaagtcctgg ccaccctgtt ttcgcataca gtagaagacc ttaaggacgc
      421 cgactggatc atcgagtcac tggacgatgt tcaggttcgg gtgctgaaca gttctgtgga
      481 aattgagttt cagccgctgt ttcaaaagca ggcttagcgg ccctcacgca gaagaaacat
      541 tggaacatcg cgagaaccct gggtaagcgc gataatctgc acttctccat cattgcagcg
      601 atagatgatg agatagttct caaccgccca gaacaaaacg ggtagtgatg tcaggtcttt
      661 cctgtggtgc ccaatgcgcg gatttcgtgc aatgacctca aaggcatcaa aaagcttatt
      721 caaccagcgg tccgccgcgt caatgctatc gctggcgata tattcccaga tttcatcaag
      781 atccaactca gcatctttgc cgagaacata tctgctcaag cacttttctt cctgcgagtc
      841 tcagatttct ttttaaaacg ggtgcgtgct gcatcgggcc caatcttctc accctgatca
      901 agcgatgtta tgcgtctctt taattcacga tgaaaagctt caaggcgggt cgcccgttga
      961 ttgtcttgct cttcaagcag acgcaacgct tcgcgcacca cttcgcttgc cgagttatag
     1021 cggcccgatg cgacctttcc gctgacaaac cgctccagtg ctggggtaag cgaaacattc
     1081 atgacgcaat ttccttgcat attatgataa cgaacattgt caatctatga cattacatcg
     1141 cgattctttt ctcagtgtgc cttcttaccc accagcaagg ccggtctccg gcccagggaa
     1201 acatggagcg aacacacgtc acttgctgcg agctgacata gtcgagttcc tctttgccgt
     1261 tttttaaggg aacagccatg aatgcattga ttttcacagt gtcaaaggcg ctgccccggg
     1321 cgaaacggat gcgatagacc agccagtctg cggccaggag caccagcagt gctatgagga
     1381 tgatgacgag ggcgcgctcc agaagaactc gcatcacgcc atcatagcgt cacatctttc
     1441 atgagagaag gcgcccattg acggccattg cccggcagcg taccttcatc acgctgccgg
     1501 actcctatgg caaaccattc atcccgccct gatagactct ggaccgtttt tgtaactctc
     1561 ctggttacat tggcaggatg gtcttccacc cagtctgtcc cgaacggccc aaataccaat
     1621 ccatctgcgg atttctatgt cgcaaccaat ggaaatgacg catggcccgg aactctgtcg
     1681 caaccgttcc gcacggtaga ccgcgcacgg cttgccgtcc aatcgttaaa gtcacacgta
     1741 agtggccgca ccatcagggt gctcatccgc ggaggcgtct actatcttcc ctctacctgg
     1801 acattcactg cgcaggactc cggaagttcc aatactcctg tcttatacgc caattacccc
     1861 aatgaaacgc ccgtcatcag cggcggccag gtacttacag gctggacgca gaatgcgaat
     1921 ggctggtggc agaccacgct tccatccgga acttacttta cgcagctctg ggtgaatgga
     1981 tcgcgccgct accgcccacg caccactcct tacggatatc tgtacatctc cggtgaatac
     2041 agcacaacag gaagcaccac cacagtaaat gagctttcct attcgaccca tgccaccaat
     2101 ggcgttccgg ctagcatggc aaacctcgga gacgtggagc tgattaactt tgaggcatgg
     2161 gacgttcctc atatgcgcat cgcatcagtg gacacaacca acaaacgcat tgtgaccacc
     2221 gcttcccttc cgaaacaaag cttctttacc ggattcattc caggccaccg cttccttctg
     2281 gaaaacgtga aggaagcgct ccgcctgccc ggccagttct atctcgaccg cccctcttct
     2341 gtacttacct atattcctga gaaaggcgag tcgttaagca cggcaatcat cgtcgcccca
     2401 cacttgcagc agatactccg cgccaccaac ctgagtgatg tcacgtttca ggggctcacc
     2461 tttgcctaca gcgactggca tccgggcaca aacggctatc tgtccggaca agcggacaat
     2521 ggtacacccg ccgcgctgag cctcaccaat tccacgggtg ttgtctttga ggccgatacc
     2581 attgaacata ctggcgctta cggaatcgaa tttgaaggca ccggcattgc cgggggcgct
     2641 tcaccctatc tcgcgcagtt ccgcgatggc ctgatcaccg acacgggcgg cggaggcatc
     2701 cgcgtcggtg ggccggcaac ttgtgctggc agttatcctc atacagacga gaatgtgccg
     2761 cagcacatct atatcggcaa caacctgatt accgggggtg gccgtgttgc ggttgtgggc
     2821 tttggcatta tgattggcga tgcccacaat attctcgttg agcacaatga gatatcggat
     2881 ttctattctg tgggcgtcgg tgtcggcttc aactggggct actcctgcaa ctttgcccat
     2941 gatgacgtgg tgcagtacaa ctacatccac gatttagggc agggcatcac cagcgacttt
     3001 ggggcggtct actatctctc cggtgtgaat acgggtaata agattctgaa caacgtcgtc
     3061 catgacatgg accaggaccc tgtgggatat ggaggatggg gcctttacct tgatgctggc
     3121 tcaaccggtg ttctggttga gaacaatctg gtctaccgga cgacggacgc aagcctgcat
     3181 gtgaattcat gggccagtcc accgcccagc cctgcacctc caccaagcat cttcaaaaac
     3241 aacattctgg cgtatggcgc aatgggcatg atggaccgcc acaacgatac cagcttcctt
     3301 tccatcgttt atgaaaacaa tatcttctat tacgacaagg cctctattca gtatggctac
     3361 tggtattgcg aaggaaaacc cgtatgcaca aactacttcc agtttgataa caacctgtat
     3421 tttgacaaag cagtaagcgg cggagagcca gcacagccat ttttcaaaac gccatacttc
     3481 cccgccaatg gaggtcagca gccccccatt acgcggctga cctttcagca atggcaaaac
     3541 gagggcgaag ataagcagtc gctttttgcc gatccgcgtt ttgttgatcc aacaccggga
     3601 accgacaact atacgctgca atccaactca ccagcgttca gcattgggtt cgttgccttt
     3661 gatccgaaac aagccggacg tttgccttct gcaacattga gcgcgccggt aaatgctcct
     3721 gcatttccac tgctgacaac gccgattaca gatttttaaa caccggcgtg ctatgcgggt
     3781 gggcgttttg ttttctgttc tgctgacttc cattccacag gagtgttttt aactggcttg
     3841 ctttccggaa agctttcact ctccggaatg gaagcgaatg cgattgcgac aggcgccgca
     3901 ttgcggacgg catgcgttcc gccgagataa agcgccttct ctccaaactg cgcatgaatg
     3961 gcatccatgg cctgggccag tttttggcgg cgctgctctt cgggaaaaaa gaccgcagtg
     4021 tgctggctgc ctggaaccag atcgtaaagc tccactccga taaatgttgg cgttccgtgc
     4081 gggcagccgg cccacatttt cttcaatgtt tccagagctg aaaaaatatt ttgtgactct
     4141 accatgcgcg tgtggcactc ccagatcttt ttctcccgcc ccacgctgaa gctcatatag
     4201 acagaaagac cacgggccca gagatctctg ccacgcagac gcgctgttgc gcggtcgagc
     4261 agtttctgag caacggcaaa cgctttctgc atcgtgcgca gttccggtga aagcacatgc
     4321 tgatgtccaa tgctctgcgg gacagaagcc ggtccatcga agtcatatcc tctgagccaa
     4381 taccagtagc gttcgccata caggctgccc cagactgcgc gcatctgctt tgcctcaagg
     4441 gcgagaagtc cgcgcacgga atggatgccc tgcacacgaa ggcgtacttc catgcggctg
     4501 ccgattccgg gaatgtctcg caatttgagc ggataaagca gatcgggcaa atcacctgca
     4561 tcaagcagga ccagcccgtt tggtttttgc atgtccgttg caatttttgc cagaagttcg
     4621 ttgggggcaa gcccaatcga acagcgcagg tactcgccta cttcttccct gatggtttcc
     4681 ttgatctttt tcgccagagc aagagcattt tccggcttgc actggctgcc agccagccga
     4741 caggcgattt cgtctacgga aagcacccga tccacgggga cacagcggtc tacagccttc
     4801 agtacaagat gatggaagcg cgcgtattcc cggtgccttg ccagcacgaa tacaatttca
     4861 gggcacagcc gccgcgcctc tttgaccact gtgcctgtcc gaatgccata tgcctttgcc
     4921 tcatagctgg ccgcgataca acaggtggtt tcagcttcca cgggaacgac ggccacgggc
     4981 ctgtcccgca gttccggacg caactgctgc tcgaccgatg cgaaatagga gttcaggtcg
     5041 aggtaaagcc agcgcacaag tccggaaacg ggaggctgca tgcttccagc ataagcgaag
     5101 ataaggcgaa aatgaaagtc gatttttcga ccatctttcc ggtacttatt tccaacccct
     5161 cgcttccgcc aaaagggacg ggacgcccgc ttcgcaggta tagcagcgca ggtccctgtt
     5221 aaagcgtttg caccatccat tccggcaggc ttgaggcaat gctggcgcgg actgcggcca
     5281 tcaggtcttc gcgcgccgca taatcgcgcg gccagagcgg tggatgaaag acgacatgcg
     5341 ctgttcccgg ggtgatgcgg atgctgcctt ttttcatcat gctttccgtt ccccagatag
     5401 agaccgggac gaccggagcg ccggtttcca ttgccagata aaacggcccc tttttgaagg
     5461 gcaacaagcg gccatcgcgg gagcgtgtac cctcaacgaa agtggtcacg tgcaatcctg
     5521 aggcaagtac ttccctgcct ttctcgacgc tctgtttggc ggtctccacg cgcccgtccc
     5581 ggtctacggg aatgaagtcg ccgagacgca tcgcatatcc cagcactgga attttcatca
     5641 atgagcgctt cagaaaaacc gatgtgcgtc cgggcaggag cggcagcaga atgggcggat
     5701 caagattcga gacgtggttt gacatgaaga tgtattgctg ccgcggaccg agacgttcca
     5761 ctccttccgc ttttacacga attccggcgg ccttgatgcc ggtgcgtgcg atccacatgg
     5821 cccagcggta cagcatggag atgtcgctgg tgagcagcgt ccacggcaga ccgatgatgg
     5881 ccgccgggat gccgaacagc agataggtga agagaactgc aagggtagaa gacatggttt
     5941 atgaacgtac gaatgttctt tgttcgagcg cgcgcggcag tttctcatag attccgtcga
     6001 agccgccatt ggagaggaca gccaccacat cgccggagcg tagctgggga acgatggaac
     6061 ggacgatggc atcagcattg gcaaagagcg cggcgctttt gccggactgc ttcagcgcgg
     6121 cgacgatcgt ttccggatgc aggcgttcat tttcaggaat gctctcctgt ttaaagaccc
     6181 ctgataacac aatgtgatca gcc
//
LOCUS       DASRBV010000295        11114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_424048, whole genome shotgun sequence.
ACCESSION   DASRBV010000295 DASRBV010000000
VERSION     DASRBV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424048"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFA02_10985"
     CDS             <1..687
                     /locus_tag="VFA02_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZU10414.1"
                     /translation="PGHHAEKEEHKHPVHESPAVMTIPLIILAILSVIGGWVGVPAAL
                     GGHNEIEHFLSPVMHSQSLSYEQVSVALNTQPQTVVNPTPQETPEGSKPLELMLAIVS
                     VAVAGTGWFFAHLLYFMKPGLPKQLAAKLRGLYTLLLNKYWVDELYGALIVTPVLLIS
                     RYVLKGLLDTGVITGGSLAVAYTTQGVGSILQRWQSGNIRSYAGWLAIGAAALLLLTY
                     FGFGMHFSFR"
     gene            830..2344
                     /locus_tag="VFA02_10990"
     CDS             830..2344
                     /locus_tag="VFA02_10990"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZU10415.1"
                     /translation="MLNDSILSLITFVPTAGAFVVAFLPRRGKAIQWFTLLVALLTFV
                     LTLHLPAHFNYGQAGFQFEQDRSWISSPNIRYHLGVDGISMWLVVLVGFLGPLGVLAS
                     WNAISVRTKEFYFLFLIQQTAMLGVFVALDMFLYYAFWELSLVPMAILIAMFGRNRGP
                     QAAIKFFLYTFIPSALLLVALLWLYSKTGTFDFAETQKFLSQYGALLSPQSLWWASLA
                     FLVAFAVKVPVFPLHGWLSDVFSEAPTAMAMVVAGKLGLYSIIRFNLGLFPEQSRQIA
                     PLMIALAVIGIIYGALVALVQEDMKRLIAFSTVGHLSFVTLGIFCFTVVGLNGSVYQI
                     INHELSGAAFLILLGILYERYGTYEMSQYGGLASKLPRMATLYVITALSLVGLPILNG
                     FVGEFLILSSSYGVHPGWTTAATTGVILSAAYMLTLIQRVFYGQESVLVASRPASDVV
                     AREGFALVPAAVLMLVLGVASPYWIRAINTAVSGLAQNVAHITSTITTTLAEKR"
     gene            2345..3814
                     /locus_tag="VFA02_10995"
     CDS             2345..3814
                     /locus_tag="VFA02_10995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZU10416.1"
                     /translation="MNMQLARILPEVILTITGVIIMLAEPMLARNASRKPLGWFAVLG
                     TLAALFASMWQLHLPDGAAYFGTVQTDAYSVFFHVLIAGIVLATLLVSLDSIDRDAES
                     VGELFALICFGAVGMMLMTSAVELLLVFVGLEISSISTYIMACFRRRSAKSPESAIKY
                     FLLGSFATAFFLYGIALVFGATGTTAIAAIAGLLTVSQAPLLAVVGLAMILIGIGFKI
                     SAAPFQVWTPDVYEGAPSPVVGLMSTAPKAAAFAVLLRILYVAFPLLHNHWQPLVWWM
                     AVLSMCVGNLGALRQQNVKRMLAYSSIAHAGYLLAAFAALSQEGIAAASFYAMTYAAM
                     NVGIFAVVSHAGGFGDRLTLIQDYRGLAYRSPLLGGAMAFFLISLIGIPFTGGFFGKF
                     YVFAAALHSGMVWLTIIGLINSGIAAYYYLKLLASVYSKPIHTDLLDGIPRPTIPLLV
                     ALVLTVAATLILGIVPGQALSLARAGANTLVPFVTTAMK"
     gene            complement(3899..4639)
                     /locus_tag="VFA02_11000"
     CDS             complement(3899..4639)
                     /locus_tag="VFA02_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10417.1"
                     /translation="MRGLLIFALVAGWTSLPVNAQRTMHAGHAGGFAAPRMGGGFHFA
                     SPPSFTQRSYPLRFTGVPQYRNRTPYNSWHYPSAPDWSHGYRPPYRDRGHRYRRPYVP
                     YFYANSTYLVPGLLNSWLDWPYYDDFDHDAGTSPQNYSDQTPSADYSNEEQQQRPEYS
                     AENVPPPPPATSAPQEPLKQAAVTLVFKDGHTQQVRNYAMTATTLYVLDEVASSGRRP
                     EIPLSSLDFQATQRANHQAGVDFALPAN"
     gene            complement(4714..7425)
                     /gene="ligD"
                     /locus_tag="VFA02_11005"
     CDS             complement(4714..7425)
                     /gene="ligD"
                     /locus_tag="VFA02_11005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZU10418.1"
                     /translation="MPKRAKKQLSPAEAVDEQLARYRAMRDFSVTAEPSGGALKVYAG
                     ELPFVVQKHAATRLHYDFRLGWRGVLKSWAVAKGPSYYTGDKRLAVEVEDHPMEYGGF
                     EGTIPKGQYGGGTVMVWDQGTWEPQPGEDVDEGFKKGRLKFILHGKKLKGKWTLVRMG
                     GRAAHESKSNWLLIKEHDEYEASADDPPITETAPNSAVTDRSIEEIASQEDHVWQSKP
                     SERKQNRSRLTRTRTKTAEAPDRESALHEFPKEKMPKFISPELATSVPEPPKGDDWLH
                     ELKLDGYRIQLRVEQQPSGKRSVKVLTRKGLDWTHRMPKVAQAAAQLPVKTALLDGEV
                     VVLDEAGKTSFAELQAAFEENRQEQLKYFAFDLLHLDGHSLRGFALVRRKAMLEGLLG
                     ELDENDTLHYSEHIRGHGDKTFRNACKLGAEGIVSKLADSKYESTRGRNWVKAKCVHQ
                     QEFVIGGFTPPSDGSYGIGSLLLGYYQGGELIYAGRTGTGFTQASRRALRKRLDALHQ
                     SKAPFKAISREESKDAIWVKPELVAAVQFATWTGDHRVRQASYQGLREDKPPEEVQRE
                     EPVTMPKPARRAGKSRLSEKENGGASVPSAKPAEKTSGVLGIRLTNPDKVLDEQSGMT
                     KQQLAEYYEAVAEYMLPHIEGRPLSIVRCPEGSGKPCFFQKHTSPGLPKGVEGVEVPE
                     KDGGTQEYVTVPGKEGLVGLAQMGVLEIHPWGARNDSLETPDRIIFDLDPDPAIEWKV
                     LAESAREVRDVLKQLGLESFVKSTGGKGLHVVAPITPNYEWPEVKQFTHNVVLALAHW
                     RPDRYLTKMTKSARKGRIFLDYLRNDRGATAVAPWSPRARKGARIAIPLRWEEIGKGN
                     PEDITVANFHVWKKRLAHDPWAELVKLRQPLTKKAMRAAASLAHKLR"
     gene            complement(7429..8154)
                     /locus_tag="VFA02_11010"
     CDS             complement(7429..8154)
                     /locus_tag="VFA02_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZU10419.1"
                     /translation="MSTRIEIGRNERVFVLTGAGISAESGLPTFRDSNGLWSGYRIEE
                     VATPEAWEANPELVWQFYSMRRRDAMKAAPNPGHYALASLESQLGENFFLCTQNVDDL
                     HERAGSHQLIHMHGELFSSRCEANCGEPVFEDRNLYESAKQIPRCKCGARIRPHIVWF
                     GEYPLGMDHIMLEIDRSDVMIVIGTSGNVHPAASFVQWARQKGPHVRTYYVGPEAPLN
                     ASAFTEVIPGKAGEVLPGLFGKK"
     gene            complement(8159..9490)
                     /locus_tag="VFA02_11015"
     CDS             complement(8159..9490)
                     /locus_tag="VFA02_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HZU10420.1"
                     /translation="MNRRRFLQGCAAMGAASALRLNAKSPGADVTLTIAPMQLEIASG
                     KTIHTVAYNGQVPGPLIRWPEGKPIIIDVHNQTGTPETVHWHGLWVPSNVDGAMEEGS
                     PMIQPQGSYRYSFTPRPAGFRWYHTHTFAGHNLKKATYSGQFGCFYIEPKENPGNYDH
                     EIFLALHDWNAYMGGGGDASMDVAYDYSTINGKMLGAGEPIRVREGQRVLFHILNASA
                     TLTHWLACSGHVMQVIAMDGNPVPMVKSVQAIRLAPAERIDVVIEMKQPGIWIFGETR
                     DDYRKAGMGIVLEYAGQQGEPKWMAPPEVLWDYKPFASTKRAVEADEFIPLVFRSKFT
                     GHGDFDHWLINGKSFPKTDTVSLKEGKRYRLVMKNESMDDHPVHLHRHSFEVTNLDGV
                     PLSGLMKDVVVVRARSTTEVDFVASNPGLTLFHCHQQSHMDFGFMMLFHYV"
     gene            complement(9487..10293)
                     /locus_tag="VFA02_11020"
     CDS             complement(9487..10293)
                     /locus_tag="VFA02_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10421.1"
                     /translation="MFLLLLVVRTAMAQDNYEIQVYGSDTVAPKNTMLELHSNFTVNG
                     SKPLAGSQYTADDTYPTNHAEHETVEITQGLTSWSEVGFYIFTSERSGQGIQWVGDHI
                     RPRVRAPDSWHWPVGVSLSTELGYVRPRFSADTWTWEIRPIVDKQIGRWYFAFNPALE
                     RSFHGPGVSKGVEFSPNVKVSYDFTRVITGGLEYYASYGRLWSFDSFHNQQQQFFPAI
                     DLNVSPNWEINFGVGVGPTASTDHLIVKAILGRRFDWSGHHEPGSSASTQ"
     gene            complement(10241..10537)
                     /locus_tag="VFA02_11025"
     CDS             complement(10241..10537)
                     /locus_tag="VFA02_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10422.1"
                     /translation="MKYLPEGMLLLVRLYQQCAAASEKQRQNWSSFFHAYIDIKGHEA
                     LLFFKRTAQEFRGELIEKKLSLAKAEIRALLPCLCCGLVSAFACGPYSDGPGQL"
     gene            10543..>11114
                     /locus_tag="VFA02_11030"
     CDS             10543..>11114
                     /locus_tag="VFA02_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZU10423.1"
                     /translation="MSFRKCVSVLLIGFGLNLFLSGCNVKPAATPAPSLRTIGFMTDF
                     DVRDDAVGICKAVMEGIAPGVRIIDITHQVTPFDIAEGARFLAGSAPYFPKDAVFVVV
                     VDPGVGSTRKAIIAKSKAGQYFVLPDNGLLTLVQDRDGIEAAREITNSQWMIGAKLSS
                     TFHGRDIFSPAAAHLARGDDWTQAGPELDVS"
BASE COUNT         2236 a         3225 c         2849 g         2804 t
ORIGIN      
        1 ccgggtcacc acgcagaaaa agaagagcac aagcatcctg tacatgaaag ccctgccgtg
       61 atgaccattc cgctcatcat cctcgccatt ctttccgtca tcggcggatg ggtcggcgtt
      121 ccggctgcgc tcggcggaca caatgagatt gaacatttcc tctctcctgt catgcactcg
      181 cagtcgctct cctatgaaca ggtcagcgtg gcgctgaaca cacagccgca gactgtcgtg
      241 aacccgacac cacaggagac ccccgaaggc agcaagcctc tcgaactgat gctggcaatc
      301 gtctcagtcg cagtcgcagg caccggatgg ttcttcgcgc acctgttgta cttcatgaaa
      361 cccggtctgc cgaagcagct tgctgcgaaa ctgcgcggac tttacacact gctgctcaat
      421 aagtactggg tagatgagct atacggagca ttgattgtta cgcctgtgct actcatctcg
      481 cgctacgtat taaaagggct gctcgacact ggggtaatta ccggaggaag cctggcagta
      541 gcgtatacaa cacagggcgt tggttcaatt ttgcagcgtt ggcagtcagg caatatccgc
      601 tcctatgccg gatggcttgc catcggtgca gcagcgctat tgctgcttac gtattttgga
      661 tttggaatgc acttctcatt tcgataagtg cgtccctcac tttaatttgt cattctgaac
      721 ggagcgtagc gaagtgaaga atccaagcga taccggcctg gaaacctcgc aaagattctt
      781 tctcccgttg gtcgtcagaa tgacaacgta atacataagg aacgtattcg tgttgaacga
      841 ttccatcttg tcgctgatta cgtttgtgcc tactgcgggc gcattcgtcg tcgctttcct
      901 gccgaggcgc ggaaaggcca tccagtggtt cacgctgctg gttgcgctgc tgacgtttgt
      961 gctgacgctg catctgcccg cacacttcaa ttatgggcag gcagggttcc agtttgagca
     1021 ggaccgctcc tggatctcca gccccaacat ccgttaccac ctcggtgtgg acggcatttc
     1081 gatgtggctg gtcgtgctgg ttgggttcct cgggcctttg ggcgtgcttg cttcctggaa
     1141 tgccatctct gtccgcacaa aagaattcta tttcctcttt ctcatccagc agacggcgat
     1201 gctgggcgta tttgtggcgc tcgatatgtt cctttactac gccttctggg agctttctct
     1261 ggtgcccatg gccatcctta tcgccatgtt tggacgcaac cgcggaccac aggcggccat
     1321 caagttcttc ctgtacacat tcattccgtc ggcgctgctg ctggttgcgc tgctgtggct
     1381 ttactcgaag actggcacct tcgattttgc agaaacacaa aaattcctgt cgcaatatgg
     1441 cgcattgctt tcaccgcaat ctttgtggtg ggcctcgcta gccttcctgg tagcctttgc
     1501 agtaaaggtc cccgtctttc cgctccatgg atggctctcc gatgtttttt ccgaggcccc
     1561 cacagcaatg gcaatggtgg tggccggcaa actgggcctt tattccatca tccgcttcaa
     1621 tcttggactc ttcccggaac agtcgcggca gattgcgccg ctgatgattg cgctggccgt
     1681 catcggcatc atctacggag cgctcgttgc tctcgtgcag gaagatatga agcgcctcat
     1741 cgccttctcg accgtcgggc acctcagctt tgtaacgctt ggcatcttct gcttcaccgt
     1801 tgtcggactc aacggctcgg tctatcaaat catcaaccac gaactgtccg gagcggcctt
     1861 cctcatcctg cttggcattc tgtatgagcg ttacggcact tacgaaatgt cccagtacgg
     1921 cggccttgct tcaaaactgc cgcgcatggc gacactctat gtcatcaccg cgctctcgct
     1981 cgtcggcctg cccattttga atggtttcgt gggcgagttt ctgattctca gctccagcta
     2041 cggagtgcat cccggctgga ccaccgctgc caccacaggc gtgattctca gcgccgccta
     2101 catgctcacg ctgattcagc gcgtctttta cggacaggaa tcagtattgg ttgccagcag
     2161 gcctgcttct gatgtggtcg cgcgcgaagg ctttgcgctt gtgcctgccg ccgtcttaat
     2221 gctggtgctc ggcgtcgctt cgccttattg gattcgcgcc attaacaccg ccgtcagcgg
     2281 actggcgcag aacgttgcgc acatcacctc gaccatcaca accacactgg cggagaagcg
     2341 ataaatgaac atgcaattgg cccgtattct ccccgaagtc atcctcacca tcactggtgt
     2401 catcatcatg ctggctgagc caatgctggc aaggaatgcc agccgcaagc cgctcggatg
     2461 gttcgccgtt cttggcaccc tggcggcgct ctttgccagc atgtggcaac tgcacctgcc
     2521 cgatggcgcg gcctacttcg gcacggtgca gaccgacgct tacagcgtct tcttccatgt
     2581 cctgattgcc ggaatcgtgc tggcgactct tctcgtctcg ctggattcca ttgaccgtga
     2641 tgccgagagc gttggcgagc tttttgcact catctgcttt ggcgcagtcg gcatgatgct
     2701 gatgacctcc gccgtcgaac tgctgctggt cttcgtcggc ctcgaaattt cgtccatttc
     2761 cacctacatc atggcctgct tccgccgccg cagcgccaaa agccctgaat cggccatcaa
     2821 gtactttctg cttggctcct tcgccacagc gttctttctc tacggaattg cacttgtctt
     2881 cggcgcaacc ggcaccacag cgattgccgc cattgccgga ctgctcaccg tcagtcaggc
     2941 gccgcttctc gccgttgttg gcctggccat gatcctcatc ggaattggct tcaagatttc
     3001 tgccgccccg ttccaggtct ggacacctga cgtctacgaa ggcgcgccct cgcccgtcgt
     3061 cggactgatg tctactgcgc ccaaggccgc ggcatttgcc gtccttctgc gtatccttta
     3121 cgtcgccttc ccactgctgc ataaccactg gcagccgctg gtctggtgga tggccgtact
     3181 ctcgatgtgc gtcggcaatc tgggtgcgct gcgacagcaa aacgtcaagc gcatgctggc
     3241 ctactcctcc atcgctcacg ccggatatct gctggctgct tttgctgcgc tttcgcagga
     3301 aggtatcgct gccgccagct tctatgctat gacttacgca gcgatgaatg tgggcatctt
     3361 tgctgttgtc agtcatgccg gaggtttcgg cgaccgtctc acgctgattc aggactaccg
     3421 cggcctcgcc taccgctcgc ctctgctggg cggggcaatg gccttttttc tgatctcact
     3481 catcggaatc ccttttacag gcgggttctt tggcaagttc tatgtttttg ccgctgcgct
     3541 gcattcggga atggtctggc tgactatcat cggcctcatc aatagtggta tcgcagctta
     3601 ctattatctg aagctgctgg catcggttta ctccaaacca atacataccg acctgcttga
     3661 cggcattccc cgtccgacta tacctctgct ggttgctctg gtccttacag ttgccgctac
     3721 gctgatactc gggattgttc ccggccaggc cctttcactt gccagggcag gagccaatac
     3781 actggttcct ttcgtgacga cggcaatgaa gtaacatggg cagcgtctgg gcaataatct
     3841 ccaggcgcta ttccctgcgt ctcgtcattc ggtccactgt ggttagtgag cacacctgtt
     3901 aattcgcggg cagggcaaaa tctactccag cctgatggtt tgctctctgc gtagcttgaa
     3961 aatcaaggct gctcaaggga atctccggcc tgcggcctga agatgctacc tcatccagca
     4021 cgtataacgt cgttgcggtc atcgcataat tacgcacctg ctgggtatgt ccgtctttaa
     4081 aaaccagcgt gacagcggct tgcttcaatg gctcttgggg agcagaggta gcgggtggcg
     4141 gaggcggaac attctccgcg ctgtattccg gacgctgttg ctgctcttcg ttactgtagt
     4201 cggcagaagg agtttgatcg ctgtagttct gaggagacgt accggcatca tgatcgaaat
     4261 catcatagta gggccagtcc aaccaggaat tcagcaggcc tggaacaaga tatgtggaat
     4321 tggcatagaa atatggcacg tatggtctgc gatagcgatg gccgcgatca cgataaggtg
     4381 gcctgtatcc gtggctccag tcaggagcag atggataatg ccaactgttg tacggagtgc
     4441 gattgcgata ttgtggcacg cccgtaaacc gcaaaggata gctacgctga gtgaaagatg
     4501 gtggagaggc aaaatgaaag cctccgccca tgcggggagc agcaaatccg ccagcgtgcc
     4561 ctgcgtgcat ggtgcgttgc gcatttaccg gaagagaagt ccagccagca accagagcga
     4621 agatcagaag gccgcgcata ttcagcccct tcacctttat tagacgcctt tccagccgcg
     4681 attgttcaaa aattttgctg tcctgaaaag accctatctc aacttatggg ccagcgacgc
     4741 tgccgcccgc attgcctttt tagtgagtgg ctgcctcagt ttcacaagtt cggcccaggg
     4801 atcatgcgca agccgcttct tccacacgtg aaaattcgca acggtgatgt cttccggatt
     4861 gcctttgcca atctcctccc atcgcagcgg aatcgcgatc cgcgcacctt tccttgctcg
     4921 cggagaccac ggcgcaaccg cagttgcccc tcgatcgttc cgcagatagt caagaaaaat
     4981 acggcctttg cgcgcagatt tggtcatctt cgtcagataa cggtccgggc gccagtgcgc
     5041 caaggcaagt accacgttat gtgtgaattg cttcacctca ggccattcgt aatttggcgt
     5101 gatgggcgca accacatgca gccctttgcc tccggtggac ttcacgaatg actctagacc
     5161 aagctgcttt aaaacatcgc gcacttcacg ggcgctttcc gccagcacct tccattcaat
     5221 cgcaggatcg ggatcaaggt cgaagatgat tctgtccggc gtttccagtg aatcgttccg
     5281 cgccccccac gggtggattt cgagcacgcc catctgtgcc agtccaacca gtccttcctt
     5341 gcccggaacg gtgacatatt cctgtgttcc gccgtccttt tcggggacct caactccttc
     5401 aactcctttc ggaagaccag ggcttgtgtg cttttggaaa aagcatggtt taccgctgcc
     5461 ttccggacaa cgcacgatgc tcagtggacg accttcaatg tgcggcagca tgtactcggc
     5521 cacggcttcg tagtattcgg cgagctgctg cttggtcatg ccgctttgct cgtcgagcac
     5581 tttgtcaggg ttcgttaggc ggatgcccaa aacgccagat gttttttcag cgggtttagc
     5641 agagggcacc gaagcgccac cgttttcttt ttcagataaa cgcgatttcc ctgctcttcg
     5701 cgcaggtttt ggcatggtca cgggttcctc gcgttgtact tcttctggcg gtttgtcttc
     5761 tctgagtccc tggtaagatg cctgccttac gcggtggtcc ccggtccatg tagcaaattg
     5821 gaccgcagcg accaactctg gtttcaccca aatggcgtct ttgctctctt cgcgactgat
     5881 tgccttaaac ggtgcttttg actggtggag tgcatccaaa cgtttgcgca gagcgcgccg
     5941 cgatgcctgc gtaaatccag ttccagtgcg gcccgcgtag atcaattccc cgccctgata
     6001 gtagcccagc agcaatgagc cgattccgta actgccatcc gaaggaggcg tgaagcctcc
     6061 gataacaaat tcctgttgat ggacgcactt cgctttcacc cagtttctac cgcgtgtgga
     6121 ttcgtatttt gaatcagcca gcttggaaac aatgccttca gcacccagct tgcatgcatt
     6181 gcggaaagtc ttatcgccgt gtccgcgaat gtgctcgctg taatgcagtg tgtcgttttc
     6241 gtcgagttcg cccagcagtc cctcaagcat ggcctttcgc cgcacaagag caaacccgcg
     6301 aagactatgt ccatcgaggt ggagcaaatc aaatgcaaaa tatttaagct gctcctgcct
     6361 gttttcctca aaggccgcct gtaattcggc aaagctggtc ttaccagcct catccagcac
     6421 tacaacttct ccgtccagca atgctgtctt tactggcaat tgtgctgcgg cttgtgcgac
     6481 tttcggcatg cggtgtgtcc agtccagccc cttgcgtgtg aggaccttta cgctacgctt
     6541 gccactcggt tgctgctcta cacgaagctg aatgcggtat ccatccagct tcagttcgtg
     6601 cagccagtcg tcgcctttcg gcggctctgg tacagatgtc gccaactccg gtgaaatgaa
     6661 cttcggcatc ttttcttttg gaaactcgtg cagtgcgctc tcgcggtccg gtgcttctgc
     6721 tgtttttgtt cgtgtgcgcg ttaaacgcga ccggttctgt tttctttctg acggcttact
     6781 ctgccagacg tggtcttcct gcgatgcaat ctcttcaata ctgcgatctg taaccgcgct
     6841 gttcggggcc gtttccgtta taggtggatc atctgccgag gcctcgtact catcgtgttc
     6901 tttaatcagc agccagtttg atttggattc gtgcgcggcg cgccctccca tgcggaccag
     6961 cgtccacttg cctttcaatt tctttccatg caggatgaat ttcagccgtc cctttttgaa
     7021 tccttcatct acatcctcgc ccggctgcgg ttcccatgtc ccctggtccc agaccatcac
     7081 cgtcccgcca ccatattggc ctttgggaat cgtgccctca aaacctccgt attccatggg
     7141 gtggtcttcc acttcgacgg caaggcgctt gtctccggtg tagtaactgg ggcctttggc
     7201 aacggcccag cttttgagta cgccgcgcca tcccaggcga aagtcgtagt gcaggcgcgt
     7261 ggccgcgtgt ttctggacaa caaaaggcaa ctctcccgca tagaccttta gcgcgccgcc
     7321 gctcggctcc gcggtcacag aaaaatcgcg catggcacga taccgggcaa gctgctcgtc
     7381 cacggcttca gcaggagaaa gctgtttttt cgcgcgcttc ggcatggatt atttcttccc
     7441 aaacaaaccg ggcaatacct cgccggcttt gcccggaatg acctcggtaa acgcgcttgc
     7501 gttcagcggt gcttccgggc cgacataata tgtacgcaca tgcggacctt tttgtctggc
     7561 ccattgcaca aagctggctg ccggatgcac attgccagac gtgccaatca caatcatcac
     7621 atcgctacgg tcaatctcga gcatgatgtg gtccatcccc agagggtact cgccaaacca
     7681 gacaatgtgc ggacgtattc tcgccccgca cttgcagcgc ggaatctgtt ttgccgattc
     7741 atataaattg cggtcttcaa aaacaggctc gccacaattc gcttcgcagc gcgaggagaa
     7801 cagttctcca tgcatatgta tgagttggtg cgaacctgca cgctcatgca gatcatctac
     7861 gttctgcgtg cagagaaaaa agttctcgcc aagctgcgat tccaacgagg ccagcgcata
     7921 gtgtccgggg ttgggtgcgg ccttcattgc atcgcggcgt cgcatggaat aaaactgcca
     7981 taccaattca ggattcgcct cccatgcttc cggtgtggct acttcttcaa tgcggtaccc
     8041 gctccacagg ccattactgt cacggaaagt cggcaggcca ctctcggcac tgatgccagc
     8101 gcctgtcaaa acgaaaacgc gttcatttcg gccaatttca attctcgtac tcattggttc
     8161 aaacgtaatg gaacaacatc atgaagccaa aatccatgtg actttgctga tgacagtgaa
     8221 acagcgtcaa cccaggatta gatgcaacaa aatctacttc tgtcgtgctc cgcgccctga
     8281 ctacgactac atctttcatc aatccgctga gaggaacgcc atcaaggttt gtgacctcaa
     8341 agctgtgacg gtgcagatgc actggatgat cgtccatact ttcgttcttc atgaccaggc
     8401 gataacgctt gccctccttc aggctgactg tgtctgtttt gggaaatgat tttccattaa
     8461 tcagccagtg gtcgaaatca ccgtgccctg taaactttga gcggaagact agcggaatga
     8521 actcatcggc ctcgaccgcc ctctttgtgc tggcaaatgg cttgtagtcc cataggactt
     8581 ccggcggggc catccatttc ggctcccctt gctgtccggc atactccagt acgatgccca
     8641 ttcctgcttt gcgataatcg tcgcgcgttt cgccgaatat ccaaataccg ggctgtttca
     8701 tttcaatgac aacatctatg cgttcggccg gcgcaaggcg aatggcctga acgctcttta
     8761 ccatcggaac aggattgccg tccatcgcaa ttacctgcat aacatgaccc gagcacgcga
     8821 gccagtgcgt cagcgtggcg ctggcattca gaatatgaaa aagcacccgc tggccttcac
     8881 gcacccggat tggctcgccc gctccgagca ttttgccatt gatggtggaa tagtcatagg
     8941 ctacatccat agaggcatcg ccgcctccgc ccatgtaggc gttccagtca tgcagtgcca
     9001 ggaaaatctc atggtcgtaa tttcctgggt tctctttcgg ctcaatgtaa aagcagccaa
     9061 actggccact gtatgtcgct ttcttcagat tgtgtcccgc aaatgtatgc gtgtgatacc
     9121 agcgaaaccc agccggtctg ggcgtgaagc tgtaacgata gctgccttgc ggttgtatca
     9181 tcggcgaacc ttcttccatg gcgccatcca cgttggatgg gacccataaa ccatgccagt
     9241 gaacagtctc cggagtgccg gtctgattat gaacatcaat gatgatgggt ttgccttcag
     9301 gccagcgaat gagcggaccg ggcacttgcc cgttgtatgc gacggtgtga atggtctttc
     9361 ctgaagcaat ctccaactgc attggcgcaa tcgtcagcgt cacatcggca cccgggcttt
     9421 ttgcgttcag acgaagggcc gaagcagcgc ccatggctgc gcatccctga agaaacctgc
     9481 gtctgttcat tgcgtgctcg cagagctgcc cggttcgtgg tgaccggacc agtcaaacct
     9541 tctgccaaga atggctttta caattagatg atctgtgctc gccgttggcc cgacacccac
     9601 gccaaagttg atctcccagt ttggcgacac attaaggtca atcgcgggaa agaactgctg
     9661 ctgctgatta tgaaagctgt cgaagctcca cagtcttccg tacgaagcgt agtattccag
     9721 accgcccgta atcacgcgcg taaagtcata gctgactttg acgttcggag aaaattcaac
     9781 gcctttggaa acaccaggac catgaaacga ccgctccagt gcaggattaa atgcgaaata
     9841 ccagcgcccg atctgtttat ctacaatagg gcgtatctcc catgtccatg tatcggctga
     9901 aaagcgcggt cgcacataac cgagctctgt agaaagactg actccgacgg gccaatgcca
     9961 actgtctggc gcgcgcacgc gcggacgaat gtggtcgccg acccactgga tgccctggcc
    10021 gctgcgctcg ctggtaaaaa tgtagaaacc cacttcagac caactggtca gcccctgcgt
    10081 gatttctacc gtctcgtgct ctgcatggtt ggttggatat gtatcatcag ctgtatattg
    10141 cgatccggca agaggcttcg atccgttcac agtgaaattg ctgtgcagct ccagcatcgt
    10201 atttttcggg gccacggtat cagaaccgta gacctggatt tcataattgt cctgggccat
    10261 cgctgtacgg accacaagca aaagcagaaa caagaccgca gcataaacag ggcaacaatg
    10321 cccgtatttc agctttcgcc aaagacaatt tcttttcaat gagttctcct cgaaactctt
    10381 gtgctgttcg cttgaaaaac agaagcgctt cgtgtccttt tatgtctatg taagcatgaa
    10441 aaaacgaaga ccagttttgt cgctgttttt ccgatgcagc cgcacactgc tgataaagtc
    10501 ttaccaacaa cagcattcct tcagggagat atttcaatgt tcatgtcctt tcgcaaatgc
    10561 gtttccgttc ttcttatcgg gtttgggtta aatctctttc tctccggatg taacgttaaa
    10621 ccggcggcga cccctgcccc ttcattgcgc accatcggtt tcatgacaga tttcgacgtg
    10681 cgtgatgatg cggttggcat ttgcaaagca gtgatggaag gaatcgctcc gggagtgaga
    10741 atcattgaca tcacacatca ggtcactccg tttgacattg cagaaggagc gcgttttctt
    10801 gccggctctg cgccatactt ccccaaagat gcagtgtttg ttgttgtcgt tgatccgggc
    10861 gtgggcagca cgcgaaaggc catcattgcg aaatcaaaag cgggccagta ttttgtcctt
    10921 ccggacaacg gcctgctgac tttggtccag gaccgcgacg ggattgaggc cgcgcgcgaa
    10981 atcaccaatt cgcagtggat gattggcgca aagctctcct cgacgtttca tggacgcgac
    11041 atcttctcac ccgcagcggc ccatcttgcg cgcggtgatg actggacgca ggctggtccg
    11101 gaacttgatg tctc
//
LOCUS       DASRBV010000296         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_627872, whole genome shotgun sequence.
ACCESSION   DASRBV010000296 DASRBV010000000
VERSION     DASRBV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627872"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFA02_11035"
     CDS             <1..439
                     /locus_tag="VFA02_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZU10424.1"
                     /translation="LAIGGYSYGGYMTNWLITQTTRFKAAVTGAGAVEHAANWGNDDE
                     TYDDAWYLGGRPWDDPELYQSEAALFQFNKVKTPVHLVGGDADIRVSYLEDVLMERAL
                     DQLGIPHQFLVFPGEGHPLDKNPWHGYIKVREELKWLEKYTGQ"
     gene            697..1767
                     /locus_tag="VFA02_11040"
     CDS             697..1767
                     /locus_tag="VFA02_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU10425.1"
                     /translation="MKRKNPAIFLVLVNVFLCGLSRAQVQTIVPPVVPGAKPVTVEHI
                     KVHGASLEGNLEGDAVDRDVIVFLPPGYAKDKHRRYPVVYALHGYSIGAEQWTHEIHV
                     PQTIEGAFAQGAKEMIVVLPDAKTLHNGSMYSSSVTTGDFEKFISHDLVEYVDSHYRT
                     IPDRRSRGLVGHSMGGYGATRIGMKHSDVFGSLYIMSPCCLSPRPVRSNPEFDKSLEA
                     IRTAEDEAKLPFFARAQLATAAAWSPDPKNPPLYLDLPTKNGEVQEDVVAKWTANAPL
                     AFIDQYIDNLRQYRAIAIDVGNQDGLRFDTAKLHEVLDKYGIANSFEIYQGTHTSAVA
                     DRFQNHVMPFFSKNLCFQPECK"
     gene            1783..>3743
                     /locus_tag="VFA02_11045"
     CDS             1783..>3743
                     /locus_tag="VFA02_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HZU10426.1"
                     /translation="MMTRIVILSAAISFSCAAAFGQSGAAASSGPIAVNVDLSQTSAP
                     VSKYEFGMFIEHLGPLVYRSLWSEMLDDRKFYFPITSQAPEAPAQTPGGPPRVQLRKW
                     HPVGPDAAVVMDKERPFVGDQSPRIQLDPAIPHGIRQSGISLVKGRKYVGRIWLRGTP
                     GSKVTVALVWGEGDSGRQTLSFTGLTSQYRKFPLSFTAQADTKDAAFEITGTGSGNFH
                     VGTVSLMPADNVQGFRPDTIALLRDLHSGFWRLPGGNFISDWSWYDSVGGIDKRPPMF
                     DYAWNAMQTNDVGLDEFMTLCKLIGTEPYITVNAGFGDAHSAAEEVEYMNGAVSTRLG
                     ALRARNGHPAPYHIKYWDIGNEPYGAWQLGRTDLKYYVLKHNEFAKAMRQADPSITLL
                     ASGSMLEDMNLTGEARAKYVGNLRALEGTEEDWTGGLLAHCWGNFDGITEHWYAQPGH
                     HFDVQKAKSLPLDAPTSAGYVNVEQTPLEFARYPANIVRLKAEEWQRYQQRFPAMLQK
                     KTFLSIDEYAYFGGGRGMGPNLKLALAYAFIFNEMLRHTDFLTMSAHTMGVSTLDYNP
                     VAATMNTTGLLFKLYGEHFVGSIPVALTGSSPQPAPKYPPGGDQPRENSGSPTYPLDM
                     FAALSPDRKFLELAVVNATETPQRFDLNV"
BASE COUNT          860 a          990 c         1104 g          789 t
ORIGIN      
        1 tcttgccatt ggcggataca gctatggcgg atacatgacc aactggctca tcacgcagac
       61 tacgcgattt aaggccgccg tcaccggcgc gggcgcagtg gaacacgcag ccaactgggg
      121 caatgatgat gagacatatg acgatgcctg gtatctgggc ggaagaccct gggacgatcc
      181 cgagctatat cagtcagagg ccgccctgtt tcaattcaac aaagtaaaga cgcccgtgca
      241 tctggtgggc ggagatgcag acattcgcgt gagctatctc gaagatgtgc tgatggaacg
      301 cgctctggac cagcttggta ttccgcatca gtttctcgtg tttccaggag aaggacatcc
      361 gctggataaa aacccctggc acggatacat caaagtccgc gaagaactga agtggctgga
      421 gaaatatacc gggcagtaat ttcaagaccg acttttggat gtccgcagtg acctgacggg
      481 atggagaatt gtcctgttgc gcagcgagcg gaggcaccgg agcggaagcg ccaggagcat
      541 cggggctggc ctgagcataa agacggcacg acgccagaat aaagatacag tcccaaacat
      601 aacagcctcc ttacccttaa gaaaagcgat ttattttgtt gcaagccatg gagtcaaacg
      661 tttatattgg ctccggtttg gagaagacct gaaaagatga agagaaagaa cccggcgatt
      721 ttcttagtgt tggtaaacgt cttcttgtgc gggctgtccc gcgcgcaggt gcagacgatc
      781 gtgcccccgg ttgttcccgg ggcaaagccc gtcacagtgg aacacatcaa ggtgcatggc
      841 gccagtctcg aaggcaatct ggagggcgat gccgtagacc gcgacgtgat tgtgtttctg
      901 ccgccaggct atgcaaagga caagcaccgg cgttaccccg tggtgtatgc cctgcatggt
      961 tattccattg gggccgagca gtggacgcac gagatccatg tgccacagac catcgaaggc
     1021 gcgtttgctc agggcgcgaa agagatgatt gtggtgcttc ccgatgcaaa gaccctgcac
     1081 aacggctcga tgtattcgag ttctgtgacg acgggcgatt tcgagaaatt tatctcgcat
     1141 gatctggtgg agtatgtgga ttcgcactac cggacgattc ccgaccgcag gagccgcgga
     1201 cttgtgggcc attccatggg aggttatggc gcgacgcgca tcggaatgaa acattccgat
     1261 gtctttggca gcctctacat catgagcccg tgctgcctct cgccacggcc tgtgcgttcg
     1321 aacccggaat ttgataaaag tctggaggcg atcaggacag cagaagacga ggcaaagctg
     1381 cccttctttg cgcgcgcaca gcttgccaca gccgcggcgt ggtcgcctga tccgaaaaat
     1441 cctccgttgt atcttgacct gccaacgaaa aacggcgaag tgcaggagga tgtggtcgca
     1501 aagtggacgg cgaacgcgcc gctggccttt atagatcaat acattgacaa tctgcgtcag
     1561 tatcgcgcca ttgcgatcga tgtgggaaac caggatggtc tgcgcttcga cacggccaaa
     1621 ttgcacgaag tgctggacaa gtacggaatc gcaaacagct ttgaaatcta tcaaggcacg
     1681 cacaccagcg cagtggccga ccgttttcag aaccatgtga tgcccttctt ttcgaagaac
     1741 ctctgctttc aacctgagtg caagtaggag gcatgcaaga cgatgatgac gcgaatcgta
     1801 attctgtctg cggcaatatc attcagttgc gcagcggcat ttggccaaag cggcgcggca
     1861 gcttcttctg ggccgatcgc agtcaatgtt gatctgtcgc aaacgtctgc gccggtgtcg
     1921 aaatacgaat tcgggatgtt tatcgagcac ctgggaccgc ttgtgtatcg cagcctgtgg
     1981 tcggagatgc tggatgacag aaaattttat tttccgatca catcacaggc gccggaagcc
     2041 ccagcgcaaa cacccggagg gccgccgcgg gtgcagttgc gaaaatggca tccagtgggg
     2101 cctgatgcag ccgtggtgat ggacaaagag aggccctttg ttggcgacca gagtccgcgc
     2161 attcaactcg accctgcaat tccgcatggc attcgccagt ctggaatctc tctggtcaaa
     2221 ggcaggaaat atgtgggccg catctggctg cgcggtactc cgggaagcaa agtgacggtg
     2281 gcgctggtct ggggtgaggg cgacagcggc aggcagacgc tttcgtttac cggacttaca
     2341 agccagtacc ggaagttccc tctcagcttt accgcccagg cagacacgaa agatgccgct
     2401 ttcgaaatca ccggaacggg cagcggcaat tttcacgtcg gcactgtgtc gcttatgcct
     2461 gcagataacg tgcaggggtt ccggcctgac accatcgcgc tgctgcgcga cctccattca
     2521 ggcttctggc ggctccccgg ggggaacttc atctccgact ggagctggta cgactcggtt
     2581 ggcggcattg ataagcgtcc gccaatgttt gactatgcct ggaatgcgat gcagacgaat
     2641 gatgtgggcc tggatgagtt catgacgctc tgcaaactga taggcaccga gccatacatc
     2701 actgtgaatg cgggtttcgg agacgcgcac tcggcggcgg aagaagtcga gtatatgaat
     2761 ggcgcggtca gtacccggct gggcgcgctg cgcgccagaa atggccatcc tgcgccttac
     2821 cacatcaagt actgggacat cggtaacgaa ccttatggtg catggcaact cggccgcaca
     2881 gatttgaagt attacgtcct gaagcacaac gagttcgcaa aagcgatgcg tcaggcggac
     2941 ccctccatca ctctgctcgc atcaggctcg atgctggagg acatgaacct gacgggagaa
     3001 gcgcgtgcca aatacgttgg caatcttcgc gcactcgaag gcacagagga agactggacc
     3061 ggtggcctgc ttgcccattg ttgggggaac tttgacggaa tcactgagca ctggtacgcg
     3121 cagcctgggc accatttcga tgtgcagaaa gcgaaaagtc ttccgctgga tgcgcctaca
     3181 agtgccggtt acgtgaatgt ggaacaaacc ccgcttgagt ttgcgcggta tccggcgaac
     3241 attgtgcggc tgaaagccga ggaatggcag aggtatcagc agcgctttcc cgctatgctc
     3301 cagaagaaaa cgtttctttc gattgatgag tatgcgtact tcggcggagg cagaggcatg
     3361 gggccgaatc taaagctcgc gcttgcctat gccttcatct ttaatgaaat gcttcggcac
     3421 acggacttcc tcaccatgtc tgcccatacg atgggcgttt ccacgctgga ctacaacccg
     3481 gtggctgcaa ctatgaacac gaccggactt ctgttcaagt tatatggcga gcactttgtt
     3541 ggctccattc cggttgcgct taccggaagc tcgccgcagc ccgcgccgaa atatcctccc
     3601 ggcggggacc agcccaggga gaactcagga agcccgacgt atccgctcga catgtttgcg
     3661 gcgctgtctc cagaccgcaa atttcttgag ttggctgttg tgaatgcgac ggagactccg
     3721 cagaggtttg atctgaatgt caa
//
LOCUS       DASRBV010000297         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_226302, whole genome shotgun sequence.
ACCESSION   DASRBV010000297 DASRBV010000000
VERSION     DASRBV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226302"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..87)
                     /locus_tag="VFA02_11050"
     tRNA            complement(12..87)
                     /locus_tag="VFA02_11050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ala,seq:ggc)
     gene            265..891
                     /locus_tag="VFA02_11055"
     CDS             265..891
                     /locus_tag="VFA02_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10427.1"
                     /translation="MTIATFAQTPAAATPPAASAPRPSEILHPSLDQVKQAMADINIS
                     RWKAPGEIRASTQQNADSIQRDLSSVLPGLLAQADAAPGSASAVFPVYRNIDALYDVL
                     LRISQTANLTAPQNEAVSIVSALDKLESARNQLADSILTNSRNAESKLANLQAAVQKA
                     AAAQKQAPPRTVIVDNTAEKSTTRHRKKTTNSSNPPNPPNTGTPSSPQ"
     gene            991..1437
                     /locus_tag="VFA02_11060"
     CDS             991..1437
                     /locus_tag="VFA02_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZU10428.1"
                     /translation="MHFGDSIFIFLLALVLFGPKKLPEIGRQIGKLLAEFRRASNEFK
                     MQIDEELRAMEHEERQKQLEKAAAESAAANQLPAETEKTPAIMPPSSGVTVTAESPYV
                     NMPKSEIASEYLEALEKEEQAKQAATMNGHASETSQESEQAPIHHG"
     gene            1430..2227
                     /gene="tatC"
                     /locus_tag="VFA02_11065"
     CDS             1430..2227
                     /gene="tatC"
                     /locus_tag="VFA02_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZU10429.1"
                     /translation="MADIVDRARAAVSERAELPGMTLLEHLEELRKRVIRAALYLIGG
                     FFVAYGFHEKIYGYMEKPIIFALQRHHLPTQLNYHNPIDGFNLYLKISFMAGCIIASP
                     FVLYEVWKFISPGLYKHEKRYVTPFMISTVGLFLAGAYFGYRWVFPGSLDFLFTFNKD
                     FNPLIEINEYTDLFLTVILGLGVTFELPILVMFLALFGIVNAKFLLKNIRYAILIIFI
                     IAAIITPTPDVLTMCVFATPMLVLYLISIVVAYLVHPSRRRKKAEAA"
     gene            2224..3180
                     /locus_tag="VFA02_11070"
     CDS             2224..3180
                     /locus_tag="VFA02_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZU10430.1"
                     /translation="MTFIRSGVPRISRRYGFRIRKSSRIFSFALLLGLMLVCLNTARA
                     AEGPFNGIRALEYTRQFVSIGPRWVGSPGHAKAEAFLKKQFAKDNLEADTFVSNTPAG
                     PQQMTNYIVRFPGKKDGVIVLASHYETNYPLRNINYVGANDGGSSTGLLIEMANHLRG
                     RTLDGYSVWLAFLDGEEAFKQWSDSDSTYGSRHLAAKWQNDGTLKRIKAFIVLDMIGD
                     KDLNIQRDSNSTGWLLDLIQRAAAKYGDQSYFFEDRTSDFDDHIPFVRRGVPSADVID
                     LEYGYHNSFWHTAQDTMDKLSAKSLSIVGNTMLETIRLLDQR"
     gene            3180..4085
                     /locus_tag="VFA02_11075"
     CDS             3180..4085
                     /locus_tag="VFA02_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HZU10431.1"
                     /translation="MIAGATLSYWIWFHVFVAALLFVDLAILHRGHHSLRLRVAWAWT
                     IFMATLACGFAIFLSKTQGHQHGLEFFSGYLIEGSLSIDNLFVFLVMFRALRLDAEEQ
                     HRVLLWGVLGAIVMRALFIALGVSLLNRFAWIHYVFGVFLLYAAFRLMREKPGEEAAP
                     GVLAWLQRRNLRGIRDNGIQGVRLEMPAFFFVILAVEATDLVFALDSIPAVLAITRDP
                     FIVYTSNIFAILGLRSLYYVLAGLLDKLRFLHYGLAVVLAFVALKMLLARWIEVPVTV
                     SLVVILVTLAIFAVASKLFPAKKSL"
     gene            complement(4288..5484)
                     /locus_tag="VFA02_11080"
     CDS             complement(4288..5484)
                     /locus_tag="VFA02_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZU10432.1"
                     /translation="MLSLLSRKSWHFIYAAVFLMLSGTFALHAQSQQPDVVVFTNGDQ
                     LSGKFVRAVGDTVVFHSDILGDVNIGWDKIKELRTAQKVAVLEKGVRIRDRHLPPNFP
                     VGTASVENQEITVQSATAAIAPIPVKNAQVVVDETTFDKQFRGHPGFLQGWNGGATAG
                     ATIVSATQNQYTFSGALNLTRVVPTVSWLDPRNRTTFNYAQSYGKITQPSYRAADGTI
                     VPSTYTKSSILHADAERDEYFSPRVYALGMVAFDHNYSQSLDLQQIYGGGLGWTPIKD
                     AKQQLDLKATTQYERQTFFNVVPPAKAEINLIASTFGANYNRKLPAGMVFNQQLLYIP
                     GWNDLHAYSVTETDTLSFPTYKNLSFTVGTTDSYLNDPPTTLPPTKRNSFQFIMGMTY
                     NIKSSY"
     gene            complement(5636..>6623)
                     /locus_tag="VFA02_11085"
     CDS             complement(5636..>6623)
                     /locus_tag="VFA02_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU10433.1"
                     /translation="KAASRRVRELSLVGHALLSTRHPVMAQIVPMRRCNLSCAYCNEY
                     DEVSKPVPLEEMLRRIDHLARLGTSIITISGGEPLLHPELDQIIARIRHHGRLAGMIT
                     NGYLLVPERIQRLNKAGLDHLQISIDNVQPDEISKKSLKVLDKKLQWLSEHADFHVNI
                     NSVVGGGIKNPQDALTIGRRALELGFTSTIGIIHDGDGQLKPLGGDERAVWNEMRSWK
                     KKNYSKFNQFQEAIANGQPNDWRCRAGSRYLYICEDGLVHYCSQQRGYPGKPLAEYTK
                     EDLRREFFTEKACAPHCTISCVHQVSYIDHWRAPQRRNATPGNAAGLVNIQL"
BASE COUNT         1465 a         1884 c         1694 g         1580 t
ORIGIN      
        1 attgataaga ttggtggacc tggtcgggat cgaaccgacg acctcttcca tgccatggaa
       61 gcgcgctccc agctgcgcca caggcccaca tttcaggagg ggtcttcatt cattctcttt
      121 gacaaaaggg ttttcgtcaa accatgctgt taacagtatt ttacaaccat tgattgcgct
      181 tttgcatcgg gagttgttat gtttccccgg catggctgcc taaaatataa aacgcatctt
      241 ctttgtgctg gatgtgctct tctgttgacc atcgccactt ttgcccagac gccggcagcg
      301 gctacgcccc cggccgcttc tgcccccaga ccatctgaaa tactccatcc atcgcttgac
      361 caggtaaagc aggcaatggc agacatcaat atctcccgct ggaaggcccc cggggagata
      421 cgcgcctcaa cacagcaaaa tgctgattcc attcagcgtg acctgagttc ggtcctgccc
      481 ggtctgctgg cccaggcaga tgcagctcct ggatccgctt cagcggtgtt ccctgtgtac
      541 cggaacattg atgctcttta cgatgtgctg ctgcgtattt ctcaaaccgc caacttgact
      601 gcgccgcaaa atgaggcggt cagtatcgtc tctgcgctcg acaagttgga atcggcgcgc
      661 aaccaactgg cggattccat cctgacgaac tccagaaatg cagagagcaa actggccaat
      721 ttgcaggctg cggtccagaa agcagccgcc gcgcaaaagc aggccccgcc caggaccgtc
      781 atcgttgata acacagcaga aaaatcaacg acacgacaca ggaagaagac aacaaactca
      841 tccaatccgc caaatccacc aaacacgggc accccttctt caccacagtg agcgtataaa
      901 gtatgcgctc tgtccgtggg cgcatctttc tgagggacaa gcctccattt gtggcctgtc
      961 ccctgcgatg agtatcataa agaacaacga atgcatttcg gcgattccat cttcatcttt
     1021 ctgctggccc tggtcctgtt cggccccaaa aaattgccgg aaattggccg tcagattggc
     1081 aaattgctgg ccgagttccg ccgcgccagc aatgaattca aaatgcagat tgatgaagag
     1141 cttcgcgcca tggagcacga ggagcgccag aagcaactgg aaaaagctgc cgccgaatca
     1201 gcggccgcaa accagcttcc ggctgagaca gagaagacgc cggcaatcat gcctccatcc
     1261 agcggagtga cggtcaccgc cgaatctcct tacgtcaata tgccaaagtc agaaattgct
     1321 tcggaatacc ttgaagcgtt ggaaaaagaa gagcaggcaa agcaggctgc cactatgaac
     1381 ggccacgcct cagaaacatc tcaggaaagc gagcaggccc caatccatca tggctgatat
     1441 tgtggaccgc gcacgggctg cggtatcaga acgcgctgaa ctccccggca tgaccctctt
     1501 ggaacatctg gaggagctgc gtaagcgggt catccgcgcc gccctctatc tcattggcgg
     1561 tttttttgtc gcttacggct tccacgagaa gatttatggc tacatggaaa agccgattat
     1621 ctttgccttg cagcggcacc acctgcccac gcaactgaat tatcacaatc ccattgacgg
     1681 ctttaatctg tatctgaaaa tcagttttat ggcgggatgc atcatcgcct cgccctttgt
     1741 gctctatgag gtatggaaat tcatctcgcc cggactttac aaacatgaaa aacgctatgt
     1801 gacgcctttc atgatctcca cggttggcct ttttctggct ggggcctact tcggataccg
     1861 ctgggtcttt cccggctctc tcgattttct ctttaccttc aacaaagact ttaaccctct
     1921 gattgaaatc aatgaataca cagacctctt cctgactgtg attctcggcc tcggggtcac
     1981 ctttgagtta ccgatcctgg tcatgtttct cgcgctcttc gggatcgtga acgcgaaatt
     2041 tctgctcaaa aacattcgct atgccatcct gattatcttc atcattgcgg ccatcattac
     2101 gcccactcct gacgtgctga ccatgtgtgt gtttgcaacg ccaatgctgg tcctgtacct
     2161 catcagcatt gttgtggctt atctggtcca tccttcgcgc cgcaggaaga aggcggaagc
     2221 agcatgacat ttatacggag cggggtgccc cgcatctcca gaagatatgg gtttcgcatc
     2281 aggaaaagct cacggatttt ctcattcgcg ctgcttttgg gcctgatgct tgtctgcctg
     2341 aataccgccc gggctgctga aggtccattc aacggcatcc gcgcccttga atacacacgc
     2401 caattcgttt ccatcggccc gcgttgggtt ggaagcccgg gtcacgccaa agccgaagcc
     2461 tttctgaaaa agcaattcgc aaaagacaat ctcgaagccg acacatttgt ctccaatact
     2521 cccgctggcc cgcaacagat gaccaactat atcgtccgct ttcccggcaa aaaagatggc
     2581 gtgattgtgc tggcctcgca ttatgagaca aactatccgc tgcgcaacat caattacgtc
     2641 ggagcaaacg acggcggctc ctctacaggg cttctcattg agatggccaa ccacctccgc
     2701 ggacgcaccc tcgatggata cagcgtctgg ctcgcctttc ttgatggcga agaggccttc
     2761 aaacagtggt ctgattctga cagcacctac ggcagccggc atctggccgc aaaatggcag
     2821 aatgacggca ccctgaagcg catcaaagca tttattgtgc tcgatatgat cggcgacaaa
     2881 gacctgaaca tccagcgcga ctccaattcc accggatggc tattggacct catccagcgc
     2941 gctgctgcga aatatggcga ccagtcctat ttctttgaag accgcaccag cgacttcgac
     3001 gatcacatcc cattcgtccg gcgcggcgtt ccgtccgctg atgtgattga tctcgaatac
     3061 ggataccaca actctttctg gcacacagcg caggacacca tggacaagct cagcgcgaaa
     3121 agtctctcca ttgttggcaa tacgatgctg gaaaccatcc ggctgctgga ccagcgctga
     3181 tgattgctgg cgccaccctt tcttactgga tatggtttca tgtgtttgtt gcggcgttgc
     3241 tgtttgtgga tctcgccata ctgcaccgcg gccatcactc tctccggtta cgcgtggcct
     3301 gggcatggac catcttcatg gcgaccttgg cctgcggttt cgccatcttt ctttccaaaa
     3361 cacaaggcca tcagcacggg ctggaattct tctcaggtta tctgattgaa ggctccctca
     3421 gcattgacaa tctgtttgtc tttctcgtga tgtttcgcgc tttgcgcctg gatgccgaag
     3481 agcagcatcg cgtccttctc tggggagtgc tcggcgccat tgtcatgcgc gccctgttta
     3541 ttgccctggg cgtgtcgcta ctcaatcgct ttgcatggat ccactatgtt tttggcgtat
     3601 ttctgcttta tgctgccttc cggctgatgc gcgagaagcc tggagaggag gccgcgcccg
     3661 gagtgctggc ctggctccag cgccgcaacc tgcgcggcat ccgggacaat gggattcagg
     3721 gcgtaaggct ggaaatgccc gcgttcttct ttgtaattct tgccgttgaa gccacggacc
     3781 tcgttttcgc gctcgattcg attcccgccg tgctggccat cacgcgcgat ccgttcattg
     3841 tttacacatc gaacatcttt gccattctcg gactgcgctc cttgtactac gtgcttgccg
     3901 gacttctgga caagctccgt tttctgcatt atgggcttgc cgtggtcctc gcctttgttg
     3961 cgttgaagat gctgctcgct cgctggatcg aggtccccgt cactgtatcg ctggttgtca
     4021 tcctggtcac actggcgatt tttgcagtag caagcaagct ttttcctgca aagaaatcac
     4081 tctgagatac ttcgagcgaa tggcatagat ttcgcccgca atatcaccct ggaaaacaac
     4141 cgccccaggc gcttcgcgca gaccctttaa gttggtcgcc atcggaaaac acaatcacat
     4201 ctgcaggcaa gagtgcggaa gatgtgaaga ggggccgtaa cggtgcgctc ataaacatcg
     4261 ctgcggcccg cttcttcgca gaaagtccta atacgaagac ttgatgttat acgtcatgcc
     4321 catgatgaac tggaatgaat tccgcttcgt tggcggaagc gttgtcggcg gatcgttcag
     4381 gtatgagtct gtcgtgccaa cggtaaagct caggttctta taggtgggga agctgagcgt
     4441 atcagtctct gtcaccgagt aggcgtgcag atcattccat ccgggaatat acagaagctg
     4501 ttggttaaac accatcccgg caggcaattt ccggttatag ttggcgccaa aggtcgaagc
     4561 aatcaggttg atttccgctt ttgccggagg cacaacgttg aaaaatgtct gcctctcgta
     4621 ttgcgtcgtg gccttcagat caagctgctg ttttgcatct ttgatgggag tccagcccag
     4681 accgccgcca taaatctgct ggagatcgag actctggctg tagttgtggt caaatgccac
     4741 cattcccagc gcatacaccc gtggcgagaa gtattcatcg cgttcggcat cagcatgcag
     4801 aatcgagctc ttcgtataag tagacgggac gatcgttcca tcggcagcgc ggtaagaggg
     4861 ctgcgtgatt ttcccgtaag actgtgcgta attaaacgtg gtgcggttcc tcggatcgag
     4921 ccagctcacc gtgggaacaa cgcgggtcag gttcagagcg cctgaaaatg tgtactggtt
     4981 ctgggtggca gaaacaatcg tcgctccggc tgtggcgcct ccattccatc cctgcagaaa
     5041 tccgggatgt ccgcgaaact gcttatcaaa tgttgtttcg tccaccacca cctgcgcatt
     5101 tttcaccgga atcggcgcaa tggccgctgt ggctgactgc accgttattt cctggttttc
     5161 aaccgaggct gtaccgacag ggaagttcgg cggcagatga cggtccctga tcctcacacc
     5221 tttttccaga acagcgactt tctgcgccgt gcgcagttcc ttgattttgt cccatccaat
     5281 gttcacatcg ccaagaatgt cggaatggaa cacaaccgta tcacccacgg cgcgcacaaa
     5341 ctttcctgaa agctggtcgc cgttggtgaa aaccaccaca tctggctgtt gcgattgagc
     5401 gtggagagca aatgttccgc tgagcatcag aaaaacggct gcatagatga aatgccagct
     5461 tttgcgggaa agaagagaaa gcatactgga cctcatactc aggaaaattt cacatcggtc
     5521 ttacacacgg aaaacacaaa cgatgtgggc ctcagcaaca cgagagtacg ggcagcacag
     5581 aacagcagtt atatcagtta cgtaatattt tcgcgcacga actgctacag aaaaattaaa
     5641 gctgaatgtt caccagcccg gcagcgttgc ccggagtggc attccggcgc tgcggagcgc
     5701 gccagtggtc aatataagaa acctgatgca cgcagctgat ggtgcagtgc ggcgcgcatg
     5761 ctttttcggt aaagaattcg cgcctcaggt cttctttggt gtactcggcc agcggcttgc
     5821 ccgggtatcc acgctgctgc gagcagtagt gtaccaaccc gtcctcgcaa atgtagaggt
     5881 aacgcgatcc ggcccggcag cgccagtcat tcggctggcc attggcaatg gcttcctgaa
     5941 actggttgaa cttggagtag tttttcttct tccagcttcg catctcgttc cataccgccc
     6001 gttcatcgcc gcccaggggc ttaagctggc catcgccgtc gtgaataatg ccgatggtcg
     6061 aggtaaagcc aagctccagc gcgcgcctcc caatggtgag cgcgtcctgg gggttcttga
     6121 tgcctccgcc gaccacggaa ttgatgttca cgtggaagtc ggcatgttct gagagccact
     6181 gcagtttctt gtccaggacc ttaaggctct ttttggaaat ctcgtcaggc tgcacgttat
     6241 caatggaaat ctggaggtga tcgagtccgg ccttgttcag gcgctgaatg cgctccggaa
     6301 ccagcagata gccgtttgtg atcatccctg caagccgtcc atggtgccgg atacgcgcaa
     6361 tgatctggtc cagctctgga tgcagcagcg gctcgccgcc cgagatggtg ataatgctcg
     6421 ttcccagccg cgccagatga tcaatacgcc gcagcatctc ctccagaggc actggctttg
     6481 agacctcgtc gtactcattg cagtaggcac acgaaagatt gcaccggcgc ataggaacaa
     6541 tctgcgccat caccggatgc cttgtcgaaa gcaacgcatg acccaccaga gacaactccc
     6601 ttacccggcg gctggcggct ttc
//
LOCUS       DASRBV010000298         8736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_530876, whole genome shotgun sequence.
ACCESSION   DASRBV010000298 DASRBV010000000
VERSION     DASRBV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530876"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..889
                     /gene="modA"
                     /locus_tag="VFA02_11090"
     CDS             146..889
                     /gene="modA"
                     /locus_tag="VFA02_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZU10434.1"
                     /translation="MYRLLLCLLFAPIAAAQNLRVAAAADLQPVLPPILAEFEAKTHI
                     HVDASYQSSATLATQIENGAPFDLFLAADLSFPQHVISAGLGETDRPIPYARGTLVLW
                     ARNDSPFRDLSVDTLRNPALKTIAIANPQHAPYGRAAEASLKNLGLYDQLKSKLVIAE
                     NIAQTAQYVESGNAQVGLISLTSAVSDRLRATGHYFIMPRGSYPPILQGAVVLKSSSH
                     KSDALRLLDFLLSTPVGRELSQRGLEPVK"
     gene            1035..1667
                     /locus_tag="VFA02_11095"
     CDS             1035..1667
                     /locus_tag="VFA02_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU10435.1"
                     /translation="MQFQQFDNEYLENLRRGDPRTEEHFTHYFSELIHLKLRSRLRSR
                     EAIEDVRQETFARVFALLRGDEGIRNASALGALVNSICNHVLLEHYRSHSRSEAMEDA
                     SEMDIQSDRADVVSEMMSQDTQKIVRGILDKLSDRDRKLLKSIFLDERDKDEVCHEFG
                     VDREYLRVLLHRAKQSFKSFYIKQTASSSSSRDKSGQMMRYVWIFAALLS"
     gene            1677..2354
                     /locus_tag="VFA02_11100"
     CDS             1677..2354
                     /locus_tag="VFA02_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZU10436.1"
                     /translation="MDHTEAANQSAVEKYLLGELGPEERDAFEEHYFTCEECATDLRV
                     TASFITHAKKELSRTSSTSVPQMSRRTFSFAAIFRPAFVVPVMAVLLVEIGFQHFVTL
                     PRLKHEAAVSLQPQILPSINLVDGGSRGAEIRTIAARSGRPFLLFVDVPTEARFNSYQ
                     CLLYSPSGKMIWQLPITAVQAANTVSIQVPAESAEAGVNTLVIQGVPSDGPPVDVVKY
                     RFLLQVR"
     gene            2393..3274
                     /locus_tag="VFA02_11105"
     CDS             2393..3274
                     /locus_tag="VFA02_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10437.1"
                     /translation="MADTPVRTFYYHADANAIGGYITRPFQGFVPSHTSVSLPMVGGF
                     IQKQRTGRKWKNIVSYTSEGTHVSGSRKDEENGGPWTTQVSTTIEGLNILDVITADRI
                     VAQLSVAHPYDGGQPVISVVGSQFINLRISGTPIEPVIKYDFFTDHDGSTEGDPKKRY
                     PKRPWLEQEAFLQKVRAQKAGAKDTYAQKYDNAPIPEWIRHYFHGLDTGLKGRDFVVC
                     SLIDQLPEIPNFPGTICGHSIYVPGFGKVFFGELIVTNHQFRLSMVRAQLGSPVGGAV
                     TAATASSNGKPYPPGGP"
     gene            3334..5772
                     /locus_tag="VFA02_11110"
     CDS             3334..5772
                     /locus_tag="VFA02_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZU10438.1"
                     /translation="MADPEALYAHVEGLIIHGDLDQAHKDAQKAIAFFSHRNPEWSWK
                     FRLIDASVFLQEGKNNSALALLSEEPPRTLAGSNIALLRKLLQVFAYSSVGQQAKANQ
                     LLSEAENLFRNNHLSSNDDPSFDARLFRNWGTVYLERGDLVHAEEMFHKGLDLIRTEN
                     GSYQKPPILLNLGVVALQQEHFDAALDWSQSAATAARPIHAPVILEEAEGNIGWAYYK
                     LGNFEEALKKFQTAEKEAKQLNAASDRMEWLNDIGLCMYRLGRPEEAESYYNTALEIS
                     RTYQDVEEEISSHLALAELFQQASNLDLANLHTEKALDLAHSSGRTSDAMDALFLKSL
                     ILARQRDTTQSIQILKSLDQDPAIRPSLQWQVEGALADLYAEMNNPRSADKWYRQSIT
                     TFETQRATIRQEESKLPFSTNAEKLYDDYIRFLANHKRTDEALQWLDLGRARTLEEGL
                     GIASPDFKSATQTPLDARAIARRVHGTILVYSLAPLESYLWAITSTGTHFFKLPPQDE
                     IDAHIRKYQQAILDSRDTLADSYADGLALYNILIKPAEKFIPKNSAVYIVPDGNLATL
                     NFETLLAPGGGVHYWIEDALITEASSLKLLASFHPEAHEQAAKKLLIMGDPISPDDKF
                     PALLNAGTEVGSIASHFSAASETILTQKDAIPSAYTKIGPGQFSYIHFVAHGTSSSAQ
                     PLDSAIVLSREPSNPDSFKLYARDIIQQPLHADLVTISACYGSGSRIYGGEGLVGLSW
                     AFLRAGAHYVIGSLWEASDAAAPQLMDRMYQELAQGHSPGIALRDAKLSLLHSQGVFR
                     KPLYWATFQLYGGA"
     gene            5922..6095
                     /locus_tag="VFA02_11115"
     CDS             5922..6095
                     /locus_tag="VFA02_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10439.1"
                     /translation="MSFLGPEKEREDSLSFEGLFLWSLSPNDTVANAPKVEKELLVER
                     VLRFLEQHGYVAA"
     gene            complement(6104..7045)
                     /locus_tag="VFA02_11120"
     CDS             complement(6104..7045)
                     /locus_tag="VFA02_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10440.1"
                     /translation="MPGINHRYLWAALIAVLLACGADAQTAPEVNPQSAQMDGYEAVL
                     KNNPEDAAARKGEVETGIAEALAARKTGQNDLALAYLLRAKYWVPDDAELLRDTGVQE
                     EAMKLYRDADDTLAQAQKLQPDDLKTLYAVARVKMDLGQMQASEEAWKQYLVLRPDDA
                     SAHYGYGVLLQMLQRRDEARAQFQKSVELAPDQSESYYRLGEIAREAGDALQAKQFYE
                     KAIAHGPAHAGAWTGLGILAFQAKRYQDAEVDFEKAVQYAPDFQTARYYHGLTLAKLG
                     RKEESTKEMAVAVQLADEQNAHNGQSRRLSDQPYRPQ"
     gene            7076..8200
                     /locus_tag="VFA02_11125"
     CDS             7076..8200
                     /locus_tag="VFA02_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10441.1"
                     /translation="MDPRLSLCLLLSLTAAAVPLRAQDAQAVQYLQAGTEAMRQGKAA
                     EAEKAFRMAIAADPQFAPAHLDLGLALLREGKPEQAASALNNSIQLDPTAQGAHLFLG
                     IAQYQRGHLDDARTALKQEIAQFPKSVEALTWLGIVELAAGDAEAATTPLDQAAKLAP
                     NDVNVLDYRGRAHNLVAAESYSRMRELDPDSWHVHRALGQNYADMGNSQAAIREYQAA
                     IAKQPNNADLYEALGAEYQKLGQFTQAEQAYESELRLSPNNPVALYNLGRIQVENGDP
                     AKGVTMLRKAAPLLQEPAPGYFYLGLGLAKLGQDNEAVTWLEKSLAGNPSNFVKQGAC
                     FQLARLYQRLHRPEDAARALAQLKQLKAQQSATSAGTSVK"
     gene            8428..>8736
                     /locus_tag="VFA02_11130"
     CDS             8428..>8736
                     /locus_tag="VFA02_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZU10442.1"
                     /translation="MTNSTRIRFHDFALLFLAALLLFSPRSSPGQLDTGSLDGTVTDT
                     SGSVIAGANVTLTEVNAGTTYQGKTSSSGYYVFPSVRPGKYILTVAYSGFRDFQKTGI
                     S"
BASE COUNT         1996 a         2619 c         2132 g         1989 t
ORIGIN      
        1 gacggccttt tggcgcaggt caagctgtcc atcggtgggc agatcattca ttccatcatc
       61 actgccgagg ccgtgcgcga aatgcgcctt caggtgggag aaacggccgc tgcattaatc
      121 aagtccactg aggtcatggt gctgcgtgta tagactactt ctctgtttgc tgttcgcccc
      181 catcgccgcc gcgcaaaatc tgcgagttgc cgcggcagca gatctccagc ccgtgctgcc
      241 gcccatcctg gctgagtttg aggcaaaaac acacatccat gtagatgcct cctatcagtc
      301 ctccgccacg ctcgctacgc agattgaaaa cggcgcgccc tttgatcttt ttctcgctgc
      361 agacctctcc ttcccgcagc atgtgatctc tgctggactg ggcgagaccg acaggcccat
      421 tccttacgcc cgcggcactt tggttttatg ggcacgcaat gactcgccct tccgtgacct
      481 ttctgtagac acgctgcgaa acccggcgct caagaccata gccattgcca atccccaaca
      541 tgctccttat ggacgcgccg ccgaagcctc cctcaaaaac ctcggccttt atgaccagct
      601 caagtctaag ctcgttatcg cagaaaacat cgcgcagaca gcgcaatatg tggaatccgg
      661 caatgcccag gtcggactga tttccctcac ctctgccgtt tccgatcgcc ttcgcgccac
      721 cggacactac ttcatcatgc cccgcggttc ctatccgccc attctgcagg gagcagtcgt
      781 tttaaaatct tcttctcata aatccgatgc gctacgcctg ctggatttcc tgctttccac
      841 cccggtcggg agagaattgt cccagcgggg actggaacca gtaaagtaaa ctcaggcgct
      901 actgagacat tcttgccaca tctctgacaa tcttttatga attttgccct cagtaccacc
      961 ccatagatgt atagttttga acattggcag tccgttcgta ttgagttgaa agactttatc
     1021 tcgaggatga tcggttgcag ttccagcagt ttgataacga gtaccttgaa aatctacgca
     1081 ggggtgatcc ccgcactgaa gaacatttca cccattattt cagcgagttg atccacctta
     1141 agttgcgctc ccgcctgcgt tccagagaag cgattgaaga tgtacgtcag gaaaccttcg
     1201 cccgcgtctt tgctctgttg cgaggagacg agggcattcg caacgcttcg gcgctcgggg
     1261 cgctcgtcaa ctccatctgc aatcacgtgc ttcttgagca ctaccgctcg cactcccgca
     1321 gtgaggccat ggaagacgcc tccgaaatgg acattcagtc agaccgcgcc gacgttgtct
     1381 ctgaaatgat gtcccaggac acacagaaga tcgtccgcgg catcctggac aagctcagcg
     1441 accgggatcg caagctgctc aagagtatct ttctggacga acgcgacaag gacgaggtct
     1501 gccatgagtt tggtgtagac cgcgagtatc tgcgggtact gctgcatcgc gccaagcaat
     1561 catttaagtc tttctatatc aagcagacag cctcatccag ctcctctcgc gacaaaagcg
     1621 gccagatgat gcgatacgtt tggattttcg ctgcactatt gagttgaggc tcaaacatgg
     1681 accatacaga ggctgccaat cagtctgcgg ttgaaaaata tctgctggga gaattgggcc
     1741 cggaagagcg tgacgccttc gaggagcatt acttcacctg cgaggaatgc gccacagacc
     1801 tccgcgtaac cgcatcattc atcacgcacg cgaaaaaaga actctcgcgc acttcctcaa
     1861 catctgttcc tcaaatgtcc cggcggactt tcagttttgc ggctattttc cgcccggctt
     1921 ttgtcgtacc tgtcatggca gtattgctcg tcgagattgg attccagcac ttcgtcactt
     1981 tgccgcgcct gaagcacgag gcagccgtct ccttgcagcc gcagattcta ccttccatca
     2041 atctcgtaga cggaggcagc cgcggggccg agattcgcac catcgccgcc cgctcaggtc
     2101 ggccgtttct attgtttgta gatgtcccca cagaagcacg cttcaacagc tatcagtgcc
     2161 tgctttactc tccttcaggc aaaatgatct ggcagcttcc cataacggcc gtgcaagcag
     2221 caaacacagt ttccatccag gtcccggcag aaagcgccga agcaggcgtg aatacgctcg
     2281 ttatacaggg agttccatcg gatggccctc ccgtagacgt ggtgaaatac cggtttttat
     2341 tgcaggtgcg ataaggcacc cctttgataa caattcacag aacggaaaga taatggcaga
     2401 tacaccagta cggaccttct actaccacgc tgacgccaat gcaatcgggg gctatattac
     2461 gcgcccattt cagggctttg tcccctcgca tacctctgtt tctctgccga tggtcggcgg
     2521 ctttatccag aagcagcgca caggccgcaa atggaaaaac atcgtcagct acacgagcga
     2581 gggcacgcac gtttccggca gccgcaagga cgaggagaac ggaggcccgt ggactacgca
     2641 ggtcagcacg accattgaag ggctgaacat cctcgatgtc attaccgctg accggattgt
     2701 ggcccagctc tccgtcgccc acccgtatga cggcggccag cctgtgattt ccgtagtggg
     2761 ctcccagttt attaacctgc gcatcagcgg cacgccaatc gagcctgtca tcaagtatga
     2821 tttctttacc gatcacgacg gctcaacgga aggcgatcca aagaaacgct acccaaagcg
     2881 tccctggctg gagcaagagg cgttcctcca aaaggtacgg gcgcaaaagg ccggcgcgaa
     2941 ggacacctat gcccaaaagt acgataacgc gcccattcct gaatggatca ggcactattt
     3001 tcacggcctg gatactgggc tgaaaggcag agacttcgta gtatgttctc tgatagacca
     3061 gttgccggaa attccaaact ttcccggaac catctgcggc cacagcatct atgttcctgg
     3121 ctttggaaag gtctttttcg gagaactgat cgtcaccaac caccagttcc ggctctcgat
     3181 ggtgcgggca caattggggt ctccggttgg aggggccgtt acggcggcca cggcaagctc
     3241 gaatggaaaa ccataccctc caggagggcc atagcgaaga cactagcttc atgcctcatt
     3301 ctgatagctg ccttttccgg ctgcagaaaa aagatggcag atcctgaggc cctttatgct
     3361 catgtagagg gccttatcat tcatggtgat ctcgaccagg cccataaaga tgcccagaag
     3421 gcaatcgcct ttttttccca tcgaaatcct gaatggtcct ggaaattccg gctgattgac
     3481 gccagcgttt ttctacagga gggaaagaat aacagtgcgc tcgccctgct ttctgaagaa
     3541 ccgccgcgca ccctggctgg atcgaatatc gcgcttttaa gaaagctgct tcaggtcttt
     3601 gcttatagct cagtcggtca gcaggccaaa gcaaatcagc tcctcagcga agctgaaaat
     3661 ttgttcagaa ataatcacct ttcctcgaac gacgacccgt cttttgacgc cagactcttc
     3721 agaaactggg gcacggtcta ccttgaacgc ggcgatctgg tccatgccga agagatgttt
     3781 cataaaggcc tggatctgat acgtacagaa aacggttcct atcaaaaacc tcccatcctg
     3841 ctgaacctcg gcgtagtcgc gctgcaacag gagcatttcg atgccgcact ggactggtca
     3901 caatctgccg caacagctgc tcgccctatc cacgcacccg taatcctcga agaagctgaa
     3961 gggaatattg ggtgggccta ttacaaactg ggaaattttg aagaagcctt aaagaaattt
     4021 cagactgccg agaaagaagc caaacagcta aatgccgctt cagaccggat ggaatggctg
     4081 aatgacatag gtttgtgcat gtatcgcctg gggcggccag aggaggcaga gagttactac
     4141 aataccgcac tggagatttc cagaacgtac caggacgtag aagaggagat ttcctcacat
     4201 cttgctcttg ctgaactctt tcagcaggcc agcaatctcg atctggcaaa cctgcataca
     4261 gagaaagccc tcgatctcgc acactcctca ggccggactt ctgatgcaat ggatgctctc
     4321 tttctgaaga gcctgattct ggcccgtcag agagatacga cccaatccat acagattctg
     4381 aaaagcctcg accaggatcc tgcaatccgg ccttctctgc aatggcaggt agagggtgct
     4441 ctggcggacc tttatgctga gatgaacaat ccacggtcgg ccgataagtg gtaccgccaa
     4501 tccatcacta cttttgagac ccaacgcgcc accatcagac aggaagaatc gaaactgccc
     4561 ttctccacca atgccgaaaa gctctacgac gattacattc gttttctcgc aaatcacaag
     4621 agaacagatg aggccctcca atggctggat ctggggcggg ctcgcacgct ggaagaggga
     4681 cttggcatcg cttctccgga cttcaagtcg gccactcaaa cgccgctgga cgcgcgtgct
     4741 atcgcccggc gcgtgcacgg caccattctg gtttacagcc tcgcccccct ggagtcctat
     4801 ctgtgggcca tcacctctac cggaacgcat tttttcaagc tgccacctca ggacgaaatt
     4861 gacgcgcaca tcaggaaata tcagcaggcc attctcgatt ccagagacac actggccgac
     4921 tcatacgccg acggcctggc cctctacaac atcctgataa aacctgcgga gaaattcatc
     4981 ccaaagaact ctgctgtcta tattgtcccc gacggaaatc tggccacgtt gaactttgaa
     5041 acattactcg ctcccggtgg cggcgtccat tactggatcg aagatgctct gatcacagag
     5101 gccagctcac tcaagctgct ggcgtcgttt catcccgaag cacatgaaca ggcagcaaag
     5161 aagttgctca tcatgggcga tccaatctcg cccgatgaca aattccctgc ccttctgaat
     5221 gcgggaacag aagtgggaag cattgccagt cacttctctg ccgcatctga gaccatcctg
     5281 acccagaaag acgccatccc ttccgcatat acaaagattg gccccggaca gttctcctat
     5341 atccactttg tcgcccatgg gaccagcagc agtgcgcagc cgcttgattc agccatcgtg
     5401 ctttcacgcg aaccatccaa tcctgattct ttcaagctgt acgcgcgcga catcatccag
     5461 caaccgctcc atgctgattt ggtcacgatt tcggcctgct acggatctgg ctcccgcatc
     5521 tatggcggtg aagggctggt tggcctgtcc tgggcgtttt tacgcgcagg agcgcattac
     5581 gtcatcgggt ctttgtggga ggcaagcgac gcggccgctc cgcaattaat ggaccgcatg
     5641 tatcaggaac tggcccaggg acactctccg ggcattgctt tgcgcgatgc caagttgtct
     5701 ctgttgcatt cacaaggggt cttcagaaaa ccgctctact gggccacttt ccagctttac
     5761 ggcggggcgt aagtttctca tatcttctga tcttgtgtaa cgtcccgcag ttttgtatca
     5821 ccatcgggat aaaagaatgt tatttccccc acgaaaatct tgcttgaaac tgcctggttt
     5881 ctcttcgctg catctaacgt tattaccgct gaggaagagg aatgtcattt ttaggtccag
     5941 aaaaagaacg tgaggattcc ctgtcctttg aagggttatt tttatggagc ctgtctccaa
     6001 acgatacagt tgcgaacgct ccaaaagtcg aaaaggaatt actcgttgaa agggtccttc
     6061 gttttctcga gcagcatgga tatgtagcgg cctgattagc tggctactgt ggccgatacg
     6121 gctggtcgga gagcctgcgc gactggccgt tgtgggcgtt ctgctcatcc gccagttgca
     6181 ccgccaccgc catctccttc gtcgattcct ccttgcgtcc gagcttcgcc agcgtcagtc
     6241 cgtggtagta gcgtgccgtc tgaaaatccg gcgcatactg cacggctttt tcaaaatcca
     6301 cttccgcatc ctgataccgc ttggcctgaa atgccaaaat gcctaggcct gtccacgctc
     6361 ctgcatgagc cgggccatga gcaatcgcct tttcataaaa ctgtttcgcc tgcaaagcat
     6421 caccggcctc gcgcgcaatt tctcccagcc ggtaataaga ttcggattgg tccggcgcaa
     6481 gctcaactga cttctgaaat tgtgcgcgcg cctcgtccct gcgttgcagc atttgcagca
     6541 gcactccgta accataatgg gcgctggcgt cgtccggacg taacaccagg tactgcttcc
     6601 acgcctcttc tgaggcctgc atctggccca ggtccatctt cacgcgtgcc acggcataaa
     6661 gcgttttcaa atcatccggc tgcagcttct gcgcttgtgc aagcgtgtcg tctgcgtcac
     6721 gataaagctt catcgcctct tcctggacgc ctgtgtcccg cagcagttcc gcatcgtccg
     6781 gcacccagta ctttgcgcgc aaaagataag ccagcgccag atcgttctgt cccgttttgc
     6841 gcgctgcaag cgcctctgca atgccggtct ccacttcacc cttgcgtgcc gctgcatcct
     6901 cggggttgtt cttcaggaca gcctcatagc catccatctg cgcgctttgc ggattcacct
     6961 ccggagcagt ctgcgcatca gccccacacg ccaaaagaac agcaattaac gcggcccata
     7021 aataccggtg attgattcca ggcatgctca cagttgtttc aaatattatg tctgcgtgga
     7081 cccacgattg tctctctgcc ttctcctcag cctcactgct gccgccgtcc ccctgcgcgc
     7141 gcaggacgca caagccgtcc aatacctcca ggcagggacc gaagccatgc gccagggaaa
     7201 agctgccgaa gcagaaaagg cctttcgcat ggccatcgcc gccgatccgc agtttgctcc
     7261 cgcgcacctc gatctcggtc ttgctctact gcgcgaaggc aagccggagc aagcagcgtc
     7321 ggcattgaac aattccattc aacttgatcc tacagcgcaa ggtgcgcact tgtttctcgg
     7381 aattgcacag tatcaacgcg gccatctcga cgacgcccgt actgccctga agcaggaaat
     7441 tgcacagttt cccaaaagcg tcgaggccct cacctggctc ggcatcgtag aactggctgc
     7501 cggtgatgct gaagcggcca ccaccccgct cgaccaggcc gcaaagctcg cacccaatga
     7561 tgtgaatgta ctggattacc gcggccgcgc acacaacctt gtagcggctg aaagctactc
     7621 gcgcatgcgt gaactcgatc ccgactcctg gcacgtgcat cgcgccctcg gccaaaacta
     7681 cgcggacatg ggcaactccc aggcagctat cagggaatac caggcagcta tcgccaaaca
     7741 acccaataat gccgatctat acgaagcgct tggcgcggaa taccagaaac taggccagtt
     7801 cacacaagcc gaacaggcct atgaaagcga gctgaggctt agtccaaaca atcccgttgc
     7861 cctctataac ctgggtcgta ttcaggtgga aaatggcgac ccggccaaag gcgtgaccat
     7921 gttgcgcaaa gctgctcctt tgctgcagga accagcgccc gggtacttct atcttggcct
     7981 ggggctcgca aaactcggcc aggacaacga agctgtcacc tggcttgaaa agtccctcgc
     8041 cggcaatcct tcaaactttg tcaaacaagg cgcctgcttc caattggccc gcctctatca
     8101 gcgcctgcat cgccccgagg acgccgcacg cgctttagct caactgaagc aattgaaagc
     8161 gcagcagtcc gccacttcag caggaacttc ggtcaagtaa tcgtttacta ttgttgcgac
     8221 aacctggccc atctccaatt gctcttccct tctcatgcct gcactctcca tgccacaagg
     8281 acatttgaaa ttttcttgac aagcgttttt caggttcgat agtcttcgtt cgctggaaaa
     8341 agacgttttt atagctcatg taagaaaacg attactcata tacccaagga cttgagaatt
     8401 tccttcaaag ttttcaggag gcactatatg acaaacagta caagaatacg tttccacgac
     8461 tttgccctgc ttttcctcgc agctctgctt ctcttctctc cccgctccag cccaggccag
     8521 ctcgatacag gctccctcga cggaactgtc acagatacca gtggcagcgt catcgctggc
     8581 gcaaacgtga cactgactga agtcaatgcc ggaaccacct atcaggggaa gacctcctca
     8641 tccggatact atgtctttcc ctctgtccgg cccggcaaat acatccttac cgtcgcctac
     8701 agcggttttc gtgattttca gaagactggc atctcc
//
LOCUS       DASRBV010000299         6083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_273481, whole genome shotgun sequence.
ACCESSION   DASRBV010000299 DASRBV010000000
VERSION     DASRBV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273481"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3211)
                     /locus_tag="VFA02_11135"
     CDS             complement(<1..3211)
                     /locus_tag="VFA02_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU10443.1"
                     /translation="MAGDGHGTEEQADYRYETTARERDGRHVMLSRIIEACAHNRFLV
                     FASVLMLTLAGIWSLQHIPLDALPDISNVQVIVHTNWIGQPPDVIEDQVTYPIVTSLL
                     AAPHVKAVRAQTMFGDSYVYVVFEDGTDLYWARSRVIEYLQQISGRLPENVHPSIGPD
                     ATGAGWVYEYAVVDKSGTHSLADLRSLQDWRLRYALETVPGVAEVASIGGYVRQYQVQ
                     LDPNKLLAYGISLSTIIDRVKASTNEVGGRVLDLSGAEYMIRGLGYLASLDDLATVAV
                     GSKNGTPVLLRDLGTVSFGPDIREGVAEWNGEGETVGGIIVMRQGMNALDVINGVKKK
                     LREIAPSLPAGVVIMPGYDRSGLIDASIKTLQRDLLEEAFVVSVVIIIFLFHFRSALI
                     AILALPIAVLAAFIPMYWLGVTSNIMSLGGIALAIGVLVDASIVMVENGFRHLSERQE
                     NNAEPVSEPERRTLLIYAAKQVGPALFFSLLIIVVSFLPVFLLEAQEGRMFRPLAWTK
                     TLAVGCSSILAITLVPILMVMLIRGKLRPERTNPISRITQAVYLPVLRWCLRHRWLTI
                     AVNLIFLISTFPLATRLGSQFMPPLFEGSALYMPTALPGISIGQAKVLLQEQDRILRS
                     FPEVASVFGTVGRSDSATDNAPLDMYDTTVMLKPREQWPAGMTYEKLIQEMDDRLQFP
                     GLSNTWTMPVENRLDMELTGIKTPLGLKVQGENVDGIQKLASQIQNILSGLPEVRSVF
                     AEKVAQGFYVNVEVNRPEAARYSLTVADIQTAVASGIGGKNIAENIEGRERYPINVRY
                     QHDFRGNIDKMRGVLIGTPSGAQIPLGQVARVSFSRGPAMIRDEDGALTGYIYINLKN
                     TDYGGFVSKADKLLHDKLTLPPNYSFQWSGEYELELRAKQRLQLILPVVFFVIFLLLY
                     LVFKSVAEALVLIFPTIYAMSGGLLLQWLLKYNFSVTVAVGYIALFGIAVETGVVMVV
                     YLHESLERKLQTGKPLTNADVEEAAIEGAVHRLRPKLMTVCAVLASLVPILWESGIGS
                     DVMKPIAAPIVGGMITSTIHVLILVPVFFVMMKERALKRG"
     gene            complement(3127..4725)
                     /locus_tag="VFA02_11140"
     CDS             complement(3127..4725)
                     /locus_tag="VFA02_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10444.1"
                     /translation="MNKYVLRTSLVWFVGLAVLAGVWAYRAHWNQPRAMNMPGAADVQ
                     PVAAGPSSDMTKPDPSMPGMSIPESKDAALAPVQLTPERMQSIGVKTGTVEYKQLSDD
                     IRATGTVDIDERLLSYVQIRFPGYIRKVFANATYQYIQKGEPLFTVYSPDLVATQQEY
                     LLARQNQKTLQSSSVDGVAAGADTLSAAAEQRLAQWDVPASEIARLKETGKPVTDLTI
                     YSPVSGYITERNALPNLYVEPSTRLYTVADLSRVWINAQIFQDDIGRLKPGDAVAITV
                     DSYPGRTFTGQIEEILPQVDMATRTVRVRLEIANPGLKLKPGMFVNVDVKSNLGRQLV
                     VPASAVFQSGTRQIVFVNHGNGSLEPKEVAIGPRVGDDFIVLKGLSPHQSIVTSATFL
                     IDSESQLQSAAGSFAPPPPGAGSNAPQSEVPTATQAKIDFTTDPSPPHKGGNVFRVKL
                     TDANGAPVAGAQVMVRSYMAAMPAMGMAAMNTSAILTDKGNGLYEGSGSLGAGGTWQV
                     TVTAQKNRQTIATKQLRVNATGGM"
     gene            complement(4722..5981)
                     /locus_tag="VFA02_11145"
     CDS             complement(4722..5981)
                     /locus_tag="VFA02_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU10445.1"
                     /translation="MNVVSVAATLVLAATLSAYGQETLASAPTPLSQLLAEAATSNPQ
                     LSAADHEAKAARQMVPQATALPDPKVTYQQFSVGSPKPFAGYTNSDFAYVGVGASQEL
                     PYPGKLRLRGEIADRDADTKQAEVEVIGAGIADAVKADYLQLAYLQQALGILRQNEAV
                     LDQLIQDATAHYRVGQGMQQDVLEAQVNRTKIVREITMHYQQMGQIQAHLKGLLNRDQ
                     QSPDIVAVNLTETALKTTSDELLALVKKNNPQIQADASAIQKQDAQVAFAKREGKPDF
                     ELGYMYQNTDRKYRDYYMLTFDVRFPRRKRVNAEITEAQEKLIASQQTLNTHLAQQLS
                     QVQQLYVQASSDEEQLKEFREGLIPQSDAAYRAILSAYASNKDQFTHVLSYFTDLLNL
                     KLEYAETLLDHETALAHLESLTGVTLR"
BASE COUNT         1363 a         1682 c         1692 g         1346 t
ORIGIN      
        1 tccctcgttt cagcgcccgt tccttcatca tgacgaagaa gacaggcacg aggatgagaa
       61 cgtggatggt cgaagtgatc atgccgccga cgatgggtgc cgcgatcggc ttcataacgt
      121 cggagccgat gccggactcc cataggattg gaaccaggct ggcgaggaca gcgcaaaccg
      181 tcatgagctt tggccgcagc cggtgcaccg ctccttcgat agcagcttct tccacgtctg
      241 cattcgtcag aggctttcca gtttgcaatt tacgctcgag cgattcatga aggtagacca
      301 ccatcaccac cccagtttcc accgcgatac caaaaagcgc gatgtaacca acagcgacag
      361 tcacgctgaa gttgtacttc aatagccatt gcagcaacaa accgccgctc atggcataga
      421 tggtcgggaa gataagcacc agtgcttcag caacggattt gaacaccaag tacagcaaca
      481 ggaagatgac aaaaaagacg accggaagaa tgagctgcaa acgctgcttt gcgcgcagtt
      541 ccagctcata ctcacccgac cactggaatg agtagttcgg aggtagagtg agcttatcgt
      601 ggagcagctt gtccgccttt gagacgaacc ctccatagtc ggtgttcttg aggttgatgt
      661 agatgtagcc ggtgagcgca ccatcttcat cgcggatcat agccggtcca cggctgaatg
      721 acacacgcgc aacttgtcct aatggaatct gcgctcctga tggcgtaccg atcaaaacgc
      781 cgcgcatctt gtcgatgttg ccgcggaagt catgttgata gcgaacattg atgggatagc
      841 gttcacggcc ttcgatgttc tctgcaatgt ttttgccgcc aatgcccgaa gcaacagcag
      901 tttgaatatc cgccactgtg aggctataac gcgcagcctc tggccgattg acctcaacat
      961 tgacgtagaa gccttgcgcg accttctctg caaagactga ccgcacctcc ggcaagccgg
     1021 agagaatgtt ttgtatttgc gaagcgagct tctggatacc atctacattc tccccctgca
     1081 ccttaaggcc gagtggtgtc ttgatgccgg tcaattccat atccaaacga ttctcgacgg
     1141 gcatcgtcca ggtattcgaa aggccgggaa actggagtct gtcgtccatc tcctgaatga
     1201 gcttttcgta ggtcatgcct gcaggccact gctcgcgcgg tttaagcatc accgttgtgt
     1261 cgtacatgtc gaggggggcg ttgtctgtgg cgctgtctga gcggccaacc gttccgaaaa
     1321 cacttgctac ttccgggaag ctacgaagaa ttctgtcctg ttcctggagc aataccttgg
     1381 cctggccaat tgagatgccg ggcagcgcag tgggcatata gagcgcggag ccctcaaaga
     1441 ggggcggcat gaactgactg cccagcctgg ttgccagcgg gaatgtggag atcaggaaga
     1501 ttagattgac cgcaatggtg agccagcgat gtctcagaca ccagcgaaga accggtagat
     1561 agacggcctg tgtgatgcgt gaaatgggat tcgtccgttc cgggcgcagc ttcccgcgaa
     1621 tcagcatcac catcaggatc ggaaccagtg ttatggcaag aatggacgaa catcctaccg
     1681 ccagggtctt cgtccaggcc agagggcgaa acattcgccc ttcctgtgct tcgagcagga
     1741 agaccggcag gaaggatacg acgataatca acagcgagaa gaagagtgca ggcccgactt
     1801 gtttggctgc atagatgagg agcgtccttc gctctggttc agataccggc tccgcgttgt
     1861 tctcctgacg ctccgagaga tggcggaagc cgttctcgac catcacaatc gaggcatcga
     1921 cgagcacgcc aatcgccagt gcaataccgc ccaaggacat gatgttcgag gtgacgccaa
     1981 gccaatacat tggaatgaag gctgcgagca cggcgatggg cagggcaaga atagcaatca
     2041 gcgcagagcg aaagtgaaaa aggaagatga tgatgacgac gctgacaacg aaggcctctt
     2101 cgagcaaatc gcgttgcagc gtcttaatcg aggcatcaat caggccggag cgatcatatc
     2161 ccggcatgat aactacacca gcgggaagcg acggcgcaat ctcgcggagc ttcttcttga
     2221 cgccgttgat cacatcgagg gcattcatcc cctggcgcat cacaatgatg ccgcccaccg
     2281 tttcaccttc accattccac tccgcaaccc cttcgcggat gtcagggccg aagctcacgg
     2341 ttccaagatc gcgcagtaga accggcgtgc cgttcttgct gccaacagca accgttgcga
     2401 gatcatccaa tgaggccaag tagccaagac cacggatcat gtactccgcg ccgctcagat
     2461 cgagcacacg tccaccgact tcattggtac ttgctttcac gcggtctatg atcgtggaaa
     2521 gggaaattcc ataagctaac agtttgttgg gatcgagctg tacctgatac tggcggacat
     2581 aaccgccgat acttgcgacc tccgcaacgc cgggaacagt ttcgagcgca tagcgcaaac
     2641 gccagtcctg taaactgcgc aagtcagcga ggctgtgtgt gccactctta tcaacgacgg
     2701 catactcata aacccagccc gctcccgtcg catcgggacc aatcgaagga tgtacatttt
     2761 caggcaacct tccgctgatc tgttgcaggt attcaatgac ccgcgagcgg gcccagtaga
     2821 ggtcagtgcc atcttcaaag accacgtaga cgtaggaatc cccaaacatt gtctgcgcgc
     2881 gcactgcctt gacatgcggc gcggcaagaa ggctggttac aatggggtac gtcacttgat
     2941 cttcgatcac atcgggcggc tggcctatcc agttggtatg aacaatgacc tggacattcg
     3001 atatatccgg caatgcgtcg agtggaatat gctgcaatga ccagatgcca gccagtgtca
     3061 gcatcagaac tgaggcgaac acaaggaagc gattgtgcgc gcaagcttca ataattcggg
     3121 aaagcatcac atgcctcccg tcgcgttcac gcgcagttgt ttcgtagcga tagtctgcct
     3181 gttcttctgt gccgtgaccg tcacctgcca tgtgccgccc gcgcccagcg atccgctgcc
     3241 ttcataaagg ccattgccct tgtccgtgag tatggcactc gtattcattg cggccatacc
     3301 cattgccggc attgcagcca tataggacct gaccatgacc tgcgcaccag ccacgggcgc
     3361 gccatttgca tccgttagct tcacacgaaa gacatttcct cccttgtgcg gagggctggg
     3421 atcggtggtg aaatcaatct tcgcctgggt ggcagtaggg acttcgcttt gaggagcgtt
     3481 gctgcctgca ccgggcggcg gcggagcaaa ggagcccgcg gcagactgca actgactctc
     3541 cgagtcgata agaaaagtgg ccgaagtaac gatggattga tgaggcgata agccttttaa
     3601 cacgatgaag tcatccccta cgcgtggccc aattgcaact tccttcggtt ccaggctgcc
     3661 gttgccgtgg ttcacaaaga caatctgccg cgttcctgat tgaaagacag ccgaagcggg
     3721 aacgacgagt tggcggccca gattgctctt tacatcgaca ttgacgaaca tacccggctt
     3781 cagcttgagt ccgggattgg caatctccag acgcacccgc acggtgcgcg tcgccatgtc
     3841 cacctgcggt agaatctctt cgatctgccc agtgaaggtg cgtccaggat aggagtccac
     3901 cgtgatggca acagcatctc cgggtttcag ccgtccgatg tcgtcctgga agatttgggc
     3961 attgatccaa acgcgtgaga gatcggcaac ggtatagagc cgcgtcgatg gctcgacgta
     4021 aagattgggc agcgcgttgc gttccgtgat gtatcccgaa accggcgaat agatggtcag
     4081 gtcggtgaca ggcttgcccg tttctttgag cctggcaatt tcgcttgccg gaacatccca
     4141 ctgcgccaat cgttgttcag ccgcagccga aagcgtatct gccccagcag ctacgccatc
     4201 cacgctgctc gactgaagcg tcttctgatt ctgcctcgca agcaggtact cctgctgcgt
     4261 tgccaccaga tcgggactgt acacggtgaa cagcggctca cccttctgaa tgtattgata
     4321 tgtcgcattc gcaaagacct tgcggatata accggggaag cgtatctgca cataagagag
     4381 cagacgctca tcaatatcca ctgttccagt cgcacggata tcatcgctca attgcttata
     4441 ttcgaccgta ccagtcttca caccgatgct ctgcattcgc tccggtgtga gctgtacggg
     4501 ggcaagagcg gcatcttttg attctggtat ggacatgccc ggcattgacg ggtctggttt
     4561 ggtcatgtcg gaggatggac cggcggctac aggctgcaca tcggcggccc cgggcatatt
     4621 catcgcacgg ggttgattcc agtgagcgcg gtaggcccaa acccccgcaa gaacggccaa
     4681 ccccacgaac caaaccagag aagttctcag gacatacttg ttcatcgcag tgtcactcct
     4741 gtcagggatt caagatgggc caacgcagtc tcgtggtcga gaagcgtttc ggcatattcg
     4801 agtttcagat tgagcagatc ggtgaagtac gagagcacat gtgtgaactg gtccttgttc
     4861 gaggcatagg cactcaatat tgcccggtag gccgcgtcgg attgagggat caaaccttcg
     4921 cggaattcct tcagttgctc ttcatcgctc gatgcctgta catagagttg ctgcacctgc
     4981 gaaagctgct gtgcaagatg ggtattgagc gtctgttgcg aagcgatgag cttttcctgc
     5041 gcctcagtga tctccgcatt gacccgcttc ctgcgcggaa agcggacatc aaaggtgagc
     5101 atgtaatagt cccggtattt gcggtcggtg ttctggtaca tatagccgag ttcaaaatca
     5161 ggtttgccct cgcgtttggc gaatgccacc tgcgcgtcct gcttctggat cgcactcgcg
     5221 tccgcctgaa tttgtggatt gttcttcttc accagagcca gcaattcatc ggaggtcgtc
     5281 ttcagcgcgg tttctgtcag gttcacagca acgatgtccg gcgattgctg gtcgcggttc
     5341 agcagtcctt tgagatgggc ctgaatctgc cccatctgct gatagtgcat ggtgatctca
     5401 cgcacaatct tggtgcggtt cacttgtgct tcaagcacat cctgctgcat tccctggccg
     5461 acccggtagt gggcggttgc gtcctgaatg agttggtcga ggacagcctc gttttgcctt
     5521 agaatgccga gtgcctgttg taaataggca agctggagat aatcggcctt cacagcgtcg
     5581 gcgatgcccg caccaatcac ttcgacttcg gcctgcttcg tgtctgcatc gcggtcagct
     5641 atttctccgc gcagccgcaa tttccccgga tagggcagtt cctgtgatgc tccgacgccg
     5701 acataggcga agtcgctatt cgtgtatccc gcaaatggct taggactacc gacgctgaac
     5761 tgctggtagg tgaccttcgg atcaggcaat gcggtggctt gcggcaccat ttgcctggct
     5821 gctttcgctt catgatcggc agcagaaagc tgagggttgc tggttgctgc ctcagccaac
     5881 agttgggaca gaggtgtggg tgcactcgcc agtgtttcct gcccgtaagc agaaagtgta
     5941 gcggcaagca caagcgtggc ggccactgag accacgttca tactgactcc aatcttgatt
     6001 gcagttcaag agatcgccgt gcgcacgttt gcacgctcgg cgaagataaa ggcaagagag
     6061 gagtgggcta gattctcagg acg
//
LOCUS       DASRBV010000300         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_628047, whole genome shotgun sequence.
ACCESSION   DASRBV010000300 DASRBV010000000
VERSION     DASRBV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628047"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFA02_11150"
     CDS             <1..383
                     /locus_tag="VFA02_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU10446.1"
                     /translation="EGQMAIAGNTVTVTRVPPATEPGYTVSTFADAMQQQYLEEYRKK
                     YPRKHFTGEPPAAMQKFAAPEGYSDSYDHFHNFFNAVRNRTPVVEDPVFGFRAAGAAL
                     LANVSVERGNVVHWNPETMKMEKA"
     gene            384..1796
                     /locus_tag="VFA02_11155"
     CDS             384..1796
                     /locus_tag="VFA02_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZU10447.1"
                     /translation="MKKHFRFLLPLLVLPVLATANAQEPGKATEFRKPDPGNGPGGQP
                     RIAFLAHRLGNDHAEGISLLDMNGDGYPDLLSGAYWYENPGPQGGEWKQHQFRTVGIH
                     NEFVSDCGEWIVDVDHDGLPDLVTAGWITNGLWWYKNPGPAATKAGQMWKAEKITDSY
                     DTEGGAFADINGDGKPDVALAHYNRAGVIWVDFSKDKPAVHHLGGREQDGHGIGIADV
                     NGDGKPDVLTTHGWFEQINADKDEWKWHGDWDLGDTGFPILGHDVNHDGKLDLIYGQG
                     HSYGLYWLEQAGTPAHPLWIRHTIDESFSQSHALALSDIDGDGQPELITGKRYRGHSG
                     NDPGSYDPVVVYAYKLPTREQLAAAKAHAGKTATFSHAKGIGDTMRVSAPEADPVFTR
                     YALSVNGTASAGTQFIVADLDGDGDLDIASAGKLGVHVLENLKINKVPKDVREETQPL
                     NRKWPFPGEGPEVEQEDGPQ"
     gene            complement(1879..2073)
                     /locus_tag="VFA02_11160"
     CDS             complement(1879..2073)
                     /locus_tag="VFA02_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HZU10448.1"
                     /translation="MKSRRAYWYWLLLLPALALMFPGIYARSGPELFGFPFFYWYQVA
                     WIILTSIISGIVYLATTERP"
     gene            complement(2070..2627)
                     /locus_tag="VFA02_11165"
     CDS             complement(2070..2627)
                     /locus_tag="VFA02_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZU10449.1"
                     /translation="MLTIGHSTLPIETFLAILKDNGVKRLVDIRTIPRSRHNPQFAQE
                     NLEKSLQKAGIQYDWQKSLGGLRHVQKDSINTGWRNASFRGYADYMQTREFATALDEL
                     LKAGPHEHTAIMCAEAVPWRCHRSLVADALLVRGLEVEDIYYDAKGHGRREPHKLTSF
                     ARVKGKHLWYPAEDGLFVSGIESKA"
BASE COUNT          639 a          747 c          866 g          585 t
ORIGIN      
        1 cggagggcca gatggcgatt gccgggaata ccgttacggt cacacgcgtc cctccggcaa
       61 ccgagccggg ctacacggtc tcgacttttg ccgacgcgat gcaacagcaa tatctggagg
      121 aataccggaa gaagtatccg cggaaacatt tcacgggcga gcctccggcg gcgatgcaga
      181 agtttgccgc gccggaagga tattcggaca gctacgacca cttccacaac ttctttaacg
      241 cggtccgcaa ccgcacgcca gtagtggaag acccggtctt tggtttccgc gcagccgggg
      301 ccgcgctgct ggccaatgtg agtgttgagc ggggcaatgt cgtccactgg aaccctgaaa
      361 ccatgaagat ggagaaggca tagatgaaaa agcattttcg gtttcttttg ccattgcttg
      421 ttctgcctgt gctggctacc gcgaacgcgc aggagccggg aaaggccacg gagtttcgca
      481 agcccgatcc aggaaatggg ccgggcgggc agccgcgcat tgcctttctg gcgcaccggc
      541 tgggcaatga ccatgccgaa ggcatttctt tgctggacat gaatggcgat gggtatcctg
      601 acctgctcag cggcgcgtac tggtatgaga atcccgggcc gcagggcggc gagtggaagc
      661 agcaccagtt ccgcacggtc ggaatccaca atgaatttgt atctgattgc ggcgaatgga
      721 tcgtagatgt ggaccacgat ggtctgcctg atctggtgac cgcgggctgg atcacaaatg
      781 ggctgtggtg gtacaagaac cccggtccgg cggccaccaa agccggacag atgtggaagg
      841 cggagaagat caccgacagc tacgatacgg aaggcggcgc attcgctgat atcaatggcg
      901 atggcaagcc tgatgttgcg cttgcgcact acaaccgcgc gggcgtcatc tgggtggact
      961 tttcaaagga caagcctgcc gtgcatcatt tgggcggacg cgaacaggac ggccacggca
     1021 tcggcattgc ggacgtcaat ggcgatggca aaccggatgt tctcaccaca catggatggt
     1081 ttgagcagat caatgcagac aaagacgagt ggaaatggca tggcgactgg gacctgggcg
     1141 atacgggttt tccgattctt ggccatgacg taaaccatga cggcaagctg gacctgattt
     1201 atggacaggg gcacagctat ggcctctact ggctggagca ggctggtact cccgcccatc
     1261 cgctatggat caggcatacg attgacgagt cgttctcgca atcgcacgcg cttgcattga
     1321 gtgatattga tggcgacggt cagccggaac tcatcaccgg aaaacggtat cgtgggcact
     1381 cgggcaatga tcctggatcg tatgatccgg ttgtggtgta tgcctataag ctgcccacaa
     1441 gagagcaact ggcggcggca aaggcccatg ccggaaaaac ggccacattt tcgcacgcca
     1501 aaggcatcgg ggacacgatg cgcgtctctg ctccggaggc ggacccggtc tttacgcggt
     1561 atgcgctctc agtcaacggg acagcgagcg cgggcacgca gttcattgtc gcggacctgg
     1621 atggagatgg cgacctggac attgccagcg ccggaaaact cggtgtgcat gtgctggaga
     1681 acctgaagat caacaaggtg cccaaggacg tgcgtgaaga aacacaaccg ctcaaccgca
     1741 agtggccgtt ccctggagaa ggtcctgagg tcgagcagga agacggtccg caatagcaga
     1801 cgaagatcag ggaggagggc ctcctccctg gttgaagcgc cagacaaaaa gcccgactgt
     1861 agttggaaat cctaaccgtt acggcctttc cgttgtggcc aggtagacga ttccagagat
     1921 gatgctggtc aggatgatcc acgctacctg ataccagtag aagaagggaa agccgaacag
     1981 ctccggaccg ctgcgcgcat agattcctgg aaacatcagg gcgagagcag gaagcagaag
     2041 aagccagtac cagtacgcac ggcgtgattt catgctttgg attctatacc ggatacaaag
     2101 aggccgtcct ctgccggata ccacagatgt ttgcctttca ctcgcgcgaa agacgtcaat
     2161 ttgtgtggtt cgcggcggcc atggcctttc gcatcgtaat agatgtcctc gacttcgagg
     2221 ccccgcacca gcagcgcatc ggctacgagc gagcgatgac agcgccatgg aacggcctct
     2281 gcgcacatga tggctgtatg ctcatgcggg cccgctttta acagctcatc gagggcggtg
     2341 gcaaattccc gtgtctgcat gtagtcggca tacccgcgaa aactggcgtt ccgccagcct
     2401 gtgttgatgg aatctttctg tacgtgacgt agtccgccga gagacttctg ccaatcatac
     2461 tgaatgccgg ctttttgcag agatttctcc agattttcct gcgcaaactg agggttgtgg
     2521 cgcgatctgg ggattgtgcg gatgtccacc agccgcttta ctccgttgtc tttgaggatg
     2581 gccagaaaag tttcgatcgg aagcgttgaa tggccgatgg tcaacatttc tcaggaatgt
     2641 ttcgaacaaa ccagtatatg tttttgcctg ccggcataga agtatttgta tcaggtgcgc
     2701 ggtcacacag ttgtaatgtc aggcggggag gatgcagaat acactcgcag atatgctacg
     2761 actttctgcg ttctttcttg ctgctttgat tgcttcggct tccaacccgc agaccatcct
     2821 ggtgcatggg catcgcg
//
LOCUS       DASRBV010000301         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_120868, whole genome shotgun sequence.
ACCESSION   DASRBV010000301 DASRBV010000000
VERSION     DASRBV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120868"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFA02_11170"
     CDS             complement(<1..1326)
                     /locus_tag="VFA02_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU10450.1"
                     /translation="MDSIAPRLLRRSLAPMTLVLALCASVACAQSLDKYVGQYRDNAE
                     PEIIYSFFREGNQLALESARIAHTPLHAVSQNTFANEGNSERYIFTIEGGKVIGVHRI
                     TEGPHGKEESTATHINGQPQHNHFRPYSREEVMVPMRDGVRLHAVILRPTDTTQPLPF
                     LMQRTPYGVDGSTSDSINSRYTELAASGYIFVMEDIRGRYGSEGQFLMNRPIADHHDP
                     NSPNENWIDETTDAYDTVAWLIKNIPNNNGRVGVLGISYPGFLAIESGIDPHPAVKAI
                     SPQAPMTDIWMGDDFFHNGAFRQTYGYDYAIGLETSKENAFSKLNEDAYDYFLQAGSF
                     AGAVKKANVGILPTWKAFFDHPAYDRYWQARAVEKHLTKVNMPTLEVGGWWDQEDMWG
                     PQAEYAALKPHDTQHEVFLVLGPWNHGEWARTTRYLGDVDFGAATTDQFR"
     gene            1476..>2778
                     /locus_tag="VFA02_11175"
     CDS             1476..>2778
                     /locus_tag="VFA02_11175"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZU10451.1"
                     /translation="MRRQGIVLLVCMLLTACNRNENRNTNGASEGRMAPGQNIAGHIE
                     PAGNIVSYADTVDRVAPAVVTVRSAKRVRAPQQFPFMNDPFFNWLFGGGPQSFGGQRP
                     PVAEEHALGSGVIVRPDGYILTNDHVIDGAEEISVDLVDRRTFKAKLVGADKPSDLAV
                     LKIEANNLPVLPLGNSDQVRVGDVCLAVGNPLGIGETVTAGIISAKGRQTDLSDGSFE
                     DFLQTDAPINQGNSGGALVNTRGELIGINSQILSPNGGNIGIGFAIPSNMAKTVMEQL
                     IAHGKVSRGQLGVTVQYINSDLAASLGLKQVQGVLVSSVQAGGPADKAGIKQGDVILQ
                     FNGTAVNDVNTLRNEVASAGAGADVTLTILRDGKQQQIRVKLGEFNPNATASMQRGGE
                     GRGAPSGRLGIQVTPVTPDVASQLGIPRNTQGLAVIAVDPSG"
BASE COUNT          561 a          668 c          939 g          610 t
ORIGIN      
        1 gcggaactgg tcggtggtgg ccgcgccgaa gtctacatcg cccagatagc gggtggtgcg
       61 ggcccattcg ccgtggttcc aggggccgag gacgaggaag acttcgtgct gcgtgtcatg
      121 cggcttgagg gcggcgtatt cggcctgcgg tccccacatg tcttcctggt cccaccatcc
      181 gccgacttcg agtgtgggca tattgacttt ggtcaggtgt ttttcgacag cgcgcgcctg
      241 ccaatatcgg tcataggcag ggtggtcaaa gaaggccttc caggtgggca gaatgccgac
      301 gttggctttt ttgacggccc cggcgaagga gcctgcctgc aggaaataat cgtaggcgtc
      361 ttcattgagt ttgctgaagg cgttttcctt gctggtctca aggccgatgg cgtagtcgta
      421 tccgtaggtc tggcggaagg cgccgttatg gaagaagtcg tctcccatcc agatgtcggt
      481 catgggggcc tgcggtgaga tggccttcac ggcggggtgt ggatcaatac cggattcgat
      541 ggcgaggaag ccggggtagg agatgcccag gacgccgacg cgtccattat tgttgggaat
      601 gtttttgatg agccatgcga cggtgtcgta tgcgtcggtg gtttcgtcaa tccagttttc
      661 gttgggcgag ttggggtcgt gatggtctgc gatggggcgg ttcatcagga attggccttc
      721 ggaaccatag cggccgcgga tgtcttccat cacgaagatg tagccactgg cggcgagttc
      781 cgtgtagcgg gagttgatgg agtcagaggt tgagccatct actccgtagg gtgttcgctg
      841 catcaggaac ggcagcggct gggttgtgtc cgtgggacgc agaatgacgg cgtgcaggcg
      901 gacaccgtcg cgcataggaa ccatgacttc ttcgcggctg taggggcgga agtggttgtg
      961 ctggggctgg ccgttgatgt gggtggcggt ggattcttcc ttaccgtgtg ggccttctgt
     1021 gatgcgatgg acgccaatga ctttgccgcc ctcaatggta aagatgtagc gctcactgtt
     1081 gccttcattt gcgaatgtat tttgcgaaac ggcatggagc ggagtgtgcg cgatgcgagc
     1141 agactcgagc gcaagctgat tgccttcgcg gaagaaggag tagatgatct caggttcggc
     1201 gttgtcgcgg tactggccaa catacttgtc gagcgattgt gcgcaggcga cagaagcaca
     1261 aagcgcaagg acgagagtca taggggcgag agaacggcgc aacaggcgag gggcaatcga
     1321 atccataaag aaaaaacata gcagaatgaa ttggatcgcg gtgctctttt gatggtggaa
     1381 gaaggctgtc ttcgcctcta tatacatccc gtcgggtgga atcgggagtc taataactgg
     1441 gctgtaaaac tttcattttc agcgaggtgc cgagcatgcg ccgtcaaggc attgttctac
     1501 tcgtttgtat gcttctgact gcctgcaacc gcaatgaaaa cagaaatacc aatggtgctt
     1561 cagaagggcg aatggccccg gggcagaaca ttgccgggca catagagcct gcgggcaata
     1621 ttgtttctta tgcggacacg gtggaccgcg ttgctcccgc ggtggtgacg gtgcgttcgg
     1681 caaaacgggt acgcgcgccg cagcagtttc cttttatgaa cgatcctttc tttaactggc
     1741 tgtttggcgg agggccgcag agttttggtg gacagcgtcc gcccgttgcg gaagagcatg
     1801 cactgggatc cggcgtgatt gtgcgtccgg atggctacat tcttaccaac gaccacgtga
     1861 ttgatggcgc agaagaaatc agcgtagacc tggtggaccg caggacattc aaggcgaagc
     1921 tggtgggggc cgacaagccg agcgacctgg ccgtgctgaa aattgaggcg aacaaccttc
     1981 ctgttctgcc tctgggaaat tccgaccagg tgcgcgtggg cgacgtttgt ctggctgttg
     2041 gcaatccgct aggcatagga gagacggtca cggcgggaat tatcagtgcc aagggacgcc
     2101 agacggacct gagcgatggc agtttcgaag attttctgca gacggatgcg cccatcaatc
     2161 agggcaattc gggcggcgcg ctggtgaata cgcgcggcga gctgattggc atcaactcgc
     2221 agattctatc gcccaacggc ggcaatatcg gcattgggtt tgctattcca tcgaacatgg
     2281 cgaagacggt gatggagcaa ctgattgcac acggcaaagt ttcgcgtggt caacttggcg
     2341 tcacggtgca atacattaac tctgatctgg cggccagtct tgggctcaag caggtgcagg
     2401 gtgtgctggt gagcagtgtg caggcgggtg gtccggcgga caaggcggga atcaagcagg
     2461 gagatgtcat tcttcaattc aatggcacag cagtcaatga tgtgaacacg ctgcgaaatg
     2521 aggtggcatc tgccggagca ggcgccgatg ttacgctcac gattctgcgc gatggcaaac
     2581 agcagcagat tcgcgtgaag ttgggcgagt tcaacccgaa tgcgacggcg tccatgcagc
     2641 gcggtggaga aggccgcgga gcaccatctg gacggctcgg cattcaggtc acgccggtca
     2701 cgccggatgt ggccagccag cttggtattc cgcgcaacac gcagggactg gcggtgattg
     2761 cggtggaccc atctggtc
//
LOCUS       DASRBV010000302         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_628454, whole genome shotgun sequence.
ACCESSION   DASRBV010000302 DASRBV010000000
VERSION     DASRBV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628454"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFA02_11180"
     CDS             <1..964
                     /locus_tag="VFA02_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZU10452.1"
                     /translation="RISIGGGGTDLPSYYREFGGFVISAAINKYVYITINKSFLPGYF
                     LKYSETEHAQSYEEIRHPLFREALTLHEAPQPLEIVSIADVPAGTGLGSSGTFTVGLL
                     HALYAWQRKRVTAEKLAREAIAIEMDRLQEPVGKQDQYIAAYGGLLCQEYHSDDSVQV
                     YPLRLSDATLRELRDSLMLFFVGQTRCASSLLDTQKKRSEQRDQAMLESLHFARQLGH
                     EIRSVLEDGHVGEFGRLMHEHWLRKRERSKGMTSERVDGLYEIARKRGGATGGKLVGA
                     GGSGFLLLHTQDRNRLRSAMMEAGASEMEFSFDFDGSVVMMRNI"
     gene            977..>2059
                     /locus_tag="VFA02_11185"
     CDS             977..>2059
                     /locus_tag="VFA02_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HZU10453.1"
                     /translation="MSTAPSPIVDLRGAREEGISLGTVFTVLKQRRRAFYAVLACFLL
                     LATLYCALATPRYQASGQVQIQKENIGSFGLDESVMGDAAGQASDALDYNVTLQTEAN
                     ILSSDTLALQVIKELHLETTQDFFPPQKSSALHRALFWKKPLEPLNVPLEQAPDRRYV
                     ALKIFASHLKIEPVTGTRLIDVRYSNPDPKLAADVVNHLISALMEYSYQSRFSATAQA
                     SNWLSAQLDDLRRQTEGLQSKAMKLERDTGTYGEDESHNVVLARLEELNQALAGAEAN
                     RILKESIYRVASSGDPELISGLAGNTAGAAPSMANSLGLIQNLRAQEASIKAELDQDK
                     VRYGPNYPRIAELQAELNSVERAVRDE"
BASE COUNT          478 a          540 c          614 g          427 t
ORIGIN      
        1 gcgcatttcg attggtggcg gaggcacgga ccttccctcg tattaccgcg aatttggcgg
       61 attcgtgatt tcggcggcaa tcaataaata tgtctacatc accattaaca agagcttcct
      121 gccgggatat tttctgaaat attccgaaac ggagcacgcg cagagctatg aagagatacg
      181 gcatcctctc tttcgtgaag cgctgacgct gcacgaagcg ccgcagccgc tggagattgt
      241 cagcattgct gatgttcctg caggtacagg gcttggttct tccggaacat ttacggttgg
      301 gttgctccat gctttgtacg cgtggcaacg aaagcgcgtc acagccgaaa aacttgcgcg
      361 tgaagccatc gcgattgaaa tggaccggct gcaggaacct gtcggcaagc aggaccagta
      421 cattgctgcc tatggagggt tgctgtgcca ggagtatcac tcagatgatt cggtgcaggt
      481 ctacccctta aggcttagtg atgcaacgct gcgggaattg cgcgactcgc tgatgctttt
      541 ctttgtaggg cagacgcgct gcgcctccag cctgctggac acccagaaga aacgctcaga
      601 gcagcgggac caggccatgc tcgaaagcct gcattttgcg cggcaactgg ggcacgagat
      661 ccggagcgtc ctcgaagacg gtcacgtggg cgagttcggc aggctgatgc acgaacattg
      721 gctacggaag cgcgagcgct ccaaaggtat gacaagtgaa cgcgtagacg gactgtatga
      781 aattgcgcgc aagcgcggcg gcgcgaccgg agggaagctg gtaggtgcgg gaggcagcgg
      841 atttctgctg ctgcacacgc aggaccgcaa ccggcttcgc tcggccatga tggaagccgg
      901 tgccagcgaa atggaattca gctttgattt tgacggctcc gttgtcatga tgcggaacat
      961 ctgaaagtga gaagacatga gcacagcgcc aagcccgatt gttgacctgc gcggcgcgcg
     1021 cgaggagggc atatctttag ggacggtgtt taccgtgttg aagcagaggc gacgcgcttt
     1081 ctatgcggta ttggcctgtt ttctcctgtt ggccactttg tattgcgcac tggccacgcc
     1141 gcgctatcag gcttcaggtc aggttcagat acaaaaggag aacatcggct cctttggcct
     1201 ggatgaaagc gtgatgggcg atgccgccgg gcaggcatct gacgcgcttg attacaacgt
     1261 gacattgcaa acagaagcga acattttgtc gtcggacacg ctggcgttgc aggtcatcaa
     1321 agaactccat ctggaaacaa cacaggactt ttttcctccc caaaaaagca gtgcattgca
     1381 cagagcattg ttctggaaaa aaccactcga gcctttaaat gttccgctgg agcaggcccc
     1441 agaccgccgc tatgtcgcac tgaagatttt tgcttcccat ctgaagattg agccggtaac
     1501 cgggacgcgg ctcattgatg tgcgctattc caaccccgat ccgaagctcg ctgctgacgt
     1561 ggtcaaccac ctgatcagtg cgttgatgga gtactcctat cagtcgcggt tttcggcgac
     1621 agcgcaggcg tccaactggc tctctgcgca attggatgat ctgcgcaggc agaccgaggg
     1681 cctgcaatca aaggccatga agcttgagcg cgacaccgga acatatggcg aagatgagtc
     1741 gcacaatgta gtgctcgcac gcctggaaga gctgaaccag gcgctggccg gggccgaagc
     1801 aaaccgcatc ctgaaggaat ccatttatcg cgtcgccagc agtggcgatc ccgaactgat
     1861 ttcgggcctg gcaggaaaca ccgcaggcgc tgcgccttcg atggcgaatt ctctgggact
     1921 gattcaaaac cttcgcgcac aggaagcaag catcaaagca gagcttgacc aggacaaggt
     1981 gcgctatgga ccgaactatc cgcgaattgc agaattacag gccgagctga acagtgtgga
     2041 aagggctgta cgcgacgaa
//
LOCUS       DASRBV010000303        20682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_531592, whole genome shotgun sequence.
ACCESSION   DASRBV010000303 DASRBV010000000
VERSION     DASRBV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 20682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20682
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531592"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFA02_11190"
     CDS             <1..855
                     /locus_tag="VFA02_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HZU10454.1"
                     /translation="IKLKVPDEAETGPVRVLQNGAQSNDARVLVAGMIATDLHIVANP
                     AVDREGNIYATLSGPRGQSTPVSVFRIGADGEMRPFVTGIMNATGLAVDPEGFLYVSS
                     RNDGVVYRVSPNGIYTQFAEGMGIATGIAFDSDQNLFVGDRSGTIFKIAPDRQIFVFA
                     TLEPSVSAYHLAFGPDETLYVTGPTTSSYDCIHAIDRDGNTTVFFRGLGRPQGLAFDI
                     SGNLYVAASWQGKRGVVRITPDREASLAIAGTGIVGLAFAPGGDLILATNTAMYHLAL
                     GVEGLRPF"
     gene            877..2121
                     /locus_tag="VFA02_11195"
     CDS             877..2121
                     /locus_tag="VFA02_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZU10455.1"
                     /translation="MKCEIIAIGSELLTPWRQDTNSLYLTERLNEIGVLVAFKTIVGD
                     RRKDLTNAIRTALGRADIVITMGGIGPTEDDLTREAWADALEVKLKRNGDLVGQLYAR
                     AAERRWTLTENNLKQADMLEGAEVLENPHGSAPGQWLDTVFAGHRKLGMLLPGPPREI
                     KPMFDEQCMPRLRSILPVRHIATRTLKAAMIGESLCDSRIAPIYTQYPDVETTILAGA
                     GDIQITLMCRHSLPERAQARVDELASRIEEELDDFVYSSQNESLEQIVLYYLEMRGAT
                     LSVAESCTGGMMAQRLTSISGSSRSFVGGAVVYSNELKTEFAGVQEELIDRRGAVSKE
                     VAAALADGIRRRCSSTYGVGITGIAGPTGGTEEKPVGLVYIALSDGAQTEVIEKIFAG
                     DRERIRYYATQQALDMVRRKLM"
     gene            2142..2774
                     /gene="plsY"
                     /locus_tag="VFA02_11200"
     CDS             2142..2774
                     /gene="plsY"
                     /locus_tag="VFA02_11200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZU10456.1"
                     /translation="MLVVPYLVIALISYLLGSVPFGYVFVRVFRKEDIRAKGSGNIGA
                     TNVIRSGAKGLGALTFLLDALKGAFAVLAARCFFPSVPAQNAAALAAFFAILGHIFPV
                     WLRFKGGKGVATALGVFLALAWIPALAGLGVFIIVFAAFRYVSLASIFAAAAFPVFAF
                     LLPHQPPYNTWMIAVILIVPVIVIAKHHQNIYRLLHGTEYRFGKSKEAAA"
     gene            2771..3778
                     /locus_tag="VFA02_11205"
     CDS             2771..3778
                     /locus_tag="VFA02_11205"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZU10457.1"
                     /translation="MSRIAVMGSGAWGTAIALSLAKRGGHEITLWSHAPEVSEAIRER
                     GENSIFLPGFPVPGNVRATSGIQEALRGAEIIISVMPSHHVRASYVQFAPHLRPGQVL
                     VSATKGVEDESLLRMSEVITGVLRGFDLQLPLGVLSGPSFAQEVAAGLPAAVTIASQD
                     VDLASRIQREFGSQTLRLYTNDDVVGVELGGALKNVIAIAAGIAAGLGLGHNSVAALI
                     TRGIAETTRLAVACGGRRETLAGLSGIGDLVLTCTGALSRNRTVGIELGRGRRLPEIL
                     AGLHGKVAEGVRTTSAALGLARRYGVEMPITEQVAAILNEEKSPQNAMRELMARPGRD
                     E"
     gene            3809..4774
                     /gene="ftsY"
                     /locus_tag="VFA02_11210"
     CDS             3809..4774
                     /gene="ftsY"
                     /locus_tag="VFA02_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZU10458.1"
                     /translation="MIQTLFGSLKEAEEPEKKSIFDRMKQAVTRTRESLSERIESVMA
                     MTREVDESALEELEMSLLASDLGVTTTMEILDNLRERAKRQQIRDGAELKSLLKEQIK
                     GILDEQQRPAPFVPAPPAVIMMVGVNGTGKTTTSGKLAAFFRAQGKTVLLCAADTFRA
                     AAIEQLEVWSQRSSVEMIKGRQGGDPSAVLFDALQAAKARGNDFLIVDTAGRLHTKGG
                     LMAELDKMRRTTQRIIPDAPHEVLLVMDATTGQNGLQQARLFTESAGVTGIVLTKLDG
                     TAKGGIAVAIARELKLPVRYVGVGEKLEDILPFDSSAFVDSLLGA"
     gene            4780..5889
                     /gene="ribD"
                     /locus_tag="VFA02_11215"
     CDS             4780..5889
                     /gene="ribD"
                     /locus_tag="VFA02_11215"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZU10459.1"
                     /translation="MQDDIRWMKRALALAQQSVGVSSPNPAVGCVLVQSDGSVVGEGF
                     HKYEEFDHAEIVALKQAGEKARGATAYVTLEPCSHRGRTGPCADALIHAGIARVVAAT
                     GDPNPAVNGQGIEKLRKAGIPVDVGVLCDEARAINDGFAKYIRTGLPFVTLKAGVSLD
                     GRIAPAPGSQKAGKPYYITSEMSRFQVQQMRHACDAIITGIGTVLADDPQLTDRSGCL
                     RRQPLLRVVLDSSLRLLLNSKLVQTAKEDVLVFCTSAAPAQKQRELEARGVRVEQVPE
                     CERRVSLEHVLRRLGELQVLSAMLEGGAQLNTAAMQAQIADKLCFFYAPQLLGENAVP
                     LFAGSARIQPEMQRISWKAIGNDALFEAYLRDPWK"
     gene            5899..6567
                     /locus_tag="VFA02_11220"
     CDS             5899..6567
                     /locus_tag="VFA02_11220"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZU10460.1"
                     /translation="MFTGLIEATGKILSIEERAGTKRIAVSAPGIAGRLRTGDSVAVS
                     GTCLTALDISEEPPVFHADLAAETVERTSLARLKPGAVVNLELPTPSGTPLGGHVVQG
                     HVDATGTLLALEPVSPGGDPAATDWWLRISVPEGVARYVVEKGSIAIEGISLTVAKFD
                     GRTLTIAIIPHTYAHTNLHTLQPGDPVNLEADVMLKFFEQRRQQEEEAGFELTLEYLI
                     ANGY"
     regulatory      6622..6824
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6925..9195
                     /gene="metE"
                     /locus_tag="VFA02_11225"
     CDS             6925..9195
                     /gene="metE"
                     /locus_tag="VFA02_11225"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZU10461.1"
                     /translation="MSLKTANLGYSRMGAHRELKFALESHWQGKSDEEALLHVADTLK
                     HNHWSTQQQAGIQFIPCNDFSLYDHVLDMLVALGATPVRFGDGEVTLQRYFDMARNSQ
                     QQPPMEMTKWLDTNYHYLVPEWSNNLTWKTDPAKLLHELAAGREHELHLRPVLIGPLS
                     LLLLGKSVDGTDPLKLLPPMLESYASLLGSLHAAGVDWIQVDEPYLVTDLTPEYQQAY
                     RTAYEYLAEKPLKIMLTTYFGGLGENLDLTFSLPVDGVHLDLVRAPEQLPTALEAIQS
                     EQVLGLGCVNGRNIWAADLDKAHALAQRAVHALGEERVVVAPSCSLLHVPVDVDVEDA
                     LPDGIRPWLSFARQKLEEVRWIAGGPQMFPEVFLERRRILQERAQAASAHVPAVRKAI
                     ADLTERDFHRAAAYRERVATQRAALGLPLFPTTTIGSFPQTAEIRKVRAKWRKGEMTT
                     AEYEAYLENEMAACIHTQEEIGLDVLVHGEFERNDMVEYFGEQLAGFAFTKNGWVQSY
                     GSRCVKPPIIYGDVFRPGPMTVRWSAKAQSLTQRPMKGMLTGPVTILQWSFVRDDTSR
                     REVAFQIALALRKEVQDLETAQIRVIQIDEPALREGLPLQRAAWKEYLEWAAAAFRLA
                     TSSVKDATQIHTHMCYSEFNDIFPAIAALDADVISIESARSRMELLQAFRESGYPNEI
                     GPGVYDIHATRVPDKAEIEELLQRALEVVSPEQLWVNPDCGLKTRNWPEVIAALKNMC
                     AAARELRQQYAVLSSR"
     gene            9275..12031
                     /gene="polA"
                     /locus_tag="VFA02_11230"
     CDS             9275..12031
                     /gene="polA"
                     /locus_tag="VFA02_11230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZU10462.1"
                     /translation="MSSANTTLAPVFLLDSMSFIFRAYHAMQRQRPMSTRTGVPTAAT
                     YVFVNMLNKLRRDFHPEYLAAIFDPSTPVFRDERAKTMTTIRKFNIKTQTFDEVAYAG
                     YKATRTETPPDLLQQLPYIRRALEAFHIPILLAEGFEADDVIGTLANKAAKKGHPVFI
                     VSNDKDMLQLVNDHVKVLNPVKDNLVLDRAKVEETLGVPPEKVIDVMALRGDTIDNVP
                     GAPGIGDKGSVELIQQFGSLEAALDRASEVKRKTYRESLENNRETILLSKELVTIHCE
                     VPIELNLDEMRTQPPDVNACRQLFSELEFTTLLKELAPAAQEKEVEHILEPTKEQIAE
                     FIAMARAQGLALAVPESELAAIAEQVSEMEAESAEEQEPELKTMSLLDIAEQAERPDP
                     GKQVCSIGVAAEPDRALLVPLDAVKALLEDASVSKHVHDLKGTMRALENHGVELRGAQ
                     DDLMLYSYLVNPTHTSHRLSDIAARFSNHPLQETGEKQLTEAVHAIHALTPMLREEVD
                     TLRTREVYETIDLPLAPVLLRMEKAGVRIDSGVLQEIGERLSVEIHRIGEEIYQRSGH
                     RFNINSPKQLGDVLFNKMGLPKPLKYGKGKVVSTAQDVLEELAEHNEVPRMVLEYRQL
                     TKLKSNYVDTLPLLADSEGRVHTTFNQVGTATGRLSSTNPNLQNIPVKTELGREIRAA
                     FIAAPGRVLISADYSQIELRLMAHFSEDPLLVKAYQTGQDIHTLTASEVFSVPVDKMT
                     KDIRNRAKAVNFGIVYGISPFGLAQQLGIDQHEARLYIETYFARYQGVRAYIDRVLED
                     VRREQRVRTLFGRVRPIPDIQSRNANLRGFAERTAINTPLQGTAADLIKLAMIRIERK
                     LTEKKLKAVMTLQVHDELLFDVPEDEADGVLALVKQEMEHVIELKVPVVAECGVGLNW
                     RDLK"
     gene            complement(12201..12926)
                     /locus_tag="VFA02_11235"
     CDS             complement(12201..12926)
                     /locus_tag="VFA02_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZU10463.1"
                     /translation="MRLLSFILGSFAAVADIFGGLVLVRSNWEKRYLRYFVALGAGFM
                     LAVAFLEMLPESMNFSVRWAPLLVLVGYCIVHLLEHTIVPHFHFGEETHHDEFLSART
                     SYSVLAGLAVHALFDGIAIASGFVLSATLGWLIFIAIFLHKVPEGFTVASVMMASGRS
                     RSTAVVAAAVLATATLLGVALINVLPSWVEAGLPLSAGVAIYVAATDLVPEVNREPGI
                     RMALVFFAGVLLFLLLRTLLPTA"
     gene            13084..14016
                     /locus_tag="VFA02_11240"
     CDS             13084..14016
                     /locus_tag="VFA02_11240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10464.1"
                     /translation="MPEIKRFLKNERKHLLHVAFELGQRCKVDILPRHFYSEIPDIHD
                     LKNSRQWRAPRSFYGIPCDMDAQVDWVEQCTRNFRDKLQNFDLHKRALQLNGMDTGFG
                     QVEADFLYCFVRRMRPARILQVGCGVSTAVCLFAAEDEGYTPEIICIEPYPDEFLKGQ
                     AQAGRIQLVDKKLQDVGFMCAEWLSAGDFFFVDSSHTLAPAGEVNVIVLEMLPRLAKG
                     VYIHFHDIYFPYDYSPVTLSGALFFPHETALLYAFLLMNPGFEMMASLAMLHHLRRND
                     LLRFFPEMKPKEFTDGLGITDGHFPCSIYLHRCA"
     gene            complement(14112..15278)
                     /locus_tag="VFA02_11245"
     CDS             complement(14112..15278)
                     /locus_tag="VFA02_11245"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZU10465.1"
                     /translation="MPGTLGILRETYPQERRVALVPRQCEALKKAGLEVILEQSAGAA
                     AGFPDELYIARGARVANREDVFQQADIIAQVRCLGANPEAGRADLRHLRPGQVLIGFA
                     EPLTAIQECSDLASAGVSLLALELIPRITRAQSMDALSSMATIAGYRAVLLAATHLPK
                     MFPLLMTAAGTITPAKVFILGAGVAGLQAIATARRLGAVVSAYDVRAAVKEQVESVGA
                     KFVVLDVEAQAAEDKSGYAKALGEDFYRKQREQLTEVLREQDVVITTAAVPGRKSPVL
                     ITAEMVSAMQPGSVIVDIAAERGGNCEMTKAGETVVHNDVTILGPVNLPASIPNHASQ
                     MFSANVAAVAKLLMNEGKITLNLEDEIIREALVTHEGRIVHTRVAELAGSGVSS"
     gene            15546..16844
                     /locus_tag="VFA02_11250"
     CDS             15546..16844
                     /locus_tag="VFA02_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU10466.1"
                     /translation="MLLSRRVGKVVLAIASLSACGVYAQQTVTISLDEAIRRAEANEP
                     AFVASMADSKVAALDRGIARSALLPNVVYHNQYLYTQGGTGSTTPRFIANNAVHEYVS
                     QGAVNETLGLAGMAEVKRADAAAAFAAAQLEVARRGLVATVVNLYYSLLAADKKVAVA
                     ERAAGEADSFVDLTQKRETAREAAHADVIKAQLEQQQRHRDLEDAKVAAEKARLELGV
                     LLFPDPRTPYVEESASIRKNLPTRAEVETAASANNPDIRSALAALRQSDAEVLAARAA
                     YLPDLGLNFTYGIDAPQFAANGPDHVRNLGYSAAVTLDIPVWDWLSTQHKVKQSEIRR
                     NAARVALTSAQRRLVAELDEFYSEAQAAHNQLASLDASVQTATESLRLTKLRYTAGEA
                     TVLEVVDAQNALTLAENAREDGRVRYELALANLQTLTGTL"
     gene            16854..18116
                     /locus_tag="VFA02_11255"
     CDS             16854..18116
                     /locus_tag="VFA02_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU10467.1"
                     /translation="MLNITGKIYGSAALALGLMLATGCGKKETAPQVEVEVQAQHPEV
                     GPMAAHITADAVLAPLAQAAIAPKISAPVKKFYVQRGSHVKAGQLLAVLENRDLEAAA
                     LDNQGSYTAAEAAYATATKAQVPEDYQKAQLDFAQAKANLELNESIVKARKQLFAEGA
                     IPGRDLDTAEATLVQAQAAYDSAKKHLDSMQTVSREAALKAAQGQLESAKGKYLGAEA
                     QVSYSEIRSPIHGVVTDRPLFAGETAAAGAPLLTVMDTSALLAKLHISQLLAQQLRIG
                     DSATVTVPGMEEPVPAKVSLISPALDPGSTTIEVWVKTENKDGALKVGTPVHVAIQTK
                     TVPDAVKVPASALLTAQDGGTSVMVMGADGVAHKKPVQVGIRDGEDVQILNGITPADM
                     VITEGAYGLDDGTKVKLAAAAAKNGDEK"
     gene            18113..>20682
                     /locus_tag="VFA02_11260"
     CDS             18113..>20682
                     /locus_tag="VFA02_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU10468.1"
                     /translation="MITSPPITQPSTQSFWLARSVRTVLFFAIVLTVAGIYLAFQVPI
                     SVFPETNFPRVVIGVDNGVMPVEQMQVTITKPIEDAVNSVPGLLTVRSTTSRGSAEVS
                     LFFNWNVDMFHTLQLVDAALAKVEQTLPPTARITTNRLTFATFPILGYSLTSDTLSQT
                     QLWELATYELKPPLNRVAGVSTVTVQGGKVPEYHIVPDMARMQASGITITDLVNAVQA
                     SNIIDSPGLYEENHQLILGLVGAQVHSAAELGQLVIKTTPGGAPVRVVDVAEVKPATM
                     PVYTVVTANGKPAVLLNITRQPSSNTVAVANAVADEVQQLQKKLPPGVKLAPFYDQSE
                     LVRESISSVRDAILIGLVLACVILFLFLRDWTSSIIAGLVIPVTVAVTILFLWMIGES
                     FNLMTLGGLAAAIGLVIDDAIVVVENIVLHRDSGETRIEAVRKAFSEITKPLIGSTIT
                     PVVVFLPLISVSGVTGSFFRALAVTMTAALLTSLLLALTWTPGLGLVLLRERHDVHHA
                     GPGKVLRRAMGWHSRLLEWSLAKPLLLAVACAVLVLGTWFAYQALGTDMLPEMDEGGF
                     VLDYIMPAGSSLTETNRVLEHVERILQSTPEVESTSRRTGLQMGLAAVTEANTGDITV
                     KLKKDRDRGIDEVMNDIREKIKTTEPELDVEFTQVLQDMIGDLSNAPEPIQIKIFAND
                     QKLLNELGERVADTIGKIPGVVDVQNGVENTISGPATGFQVDPTVAARLGFTPQEVSQ
                     DATSILDGIATANPLIANNRPYTIRVRLGDASRTSLEAIENTVFNSSSGHTATLGSLA
                     QIQQLPPQNEIRRENLQQMIVVTGRLEGSDLGTAMAQVQRAVENLHLPSNVRVTYGGT
                     "
BASE COUNT         4600 a         5264 c         6295 g         4523 t
ORIGIN      
        1 atcaagctca aggtcccgga tgaagctgag actgggccag tgcgcgtgct gcagaatggc
       61 gcccagagca acgatgcccg tgtgctggtg gccgggatga ttgctacaga tttgcatatt
      121 gtggcgaatc cggccgtaga ccgtgaggga aacatctatg ccacgctttc tggtccgcgc
      181 gggcagagca ctccggtttc ggttttccgc attggtgcgg atggcgaaat gaggccgttt
      241 gtcaccggaa tcatgaacgc gaccgggctg gcggtggacc ctgaaggctt tctctatgtc
      301 tcgtcgcgga acgatggggt tgtgtaccga gtttcgccga acggcattta tacgcaattt
      361 gccgagggca tggggatcgc cacgggaatt gcatttgaca gcgaccaaaa cctgtttgtt
      421 ggcgaccgaa gcggcacaat cttcaaaatt gctccggacc gccagatttt tgtctttgcc
      481 acgctggagc cgagcgtttc tgcttatcat ctcgcctttg ggccggatga aacgctttac
      541 gttaccggcc cgacaacgtc aagttatgac tgcatccacg cgatcgaccg cgatgggaac
      601 acgacggtct tctttcgcgg gctgggacga ccacagggcc tggcgttcga catcagcgga
      661 aacctttacg tagcggcatc ctggcaagga aagcgcggtg tggtgcggat tacgcctgac
      721 cgcgaggctt cgctggccat tgccggaaca ggaattgtgg gtctggcgtt tgctcctggc
      781 ggtgacctga ttctggcaac gaacacggcc atgtatcatt tagctttggg agtggaaggg
      841 ctgcggcctt tctgagtcct tgacccctgc ctgctcatga aatgcgagat tattgctatt
      901 ggttctgaac tgctcacccc ctggcggcag gacacgaact ctctctacct gacggaacgc
      961 ctgaacgaaa tcggtgtgct cgtcgctttt aagaccattg ttggcgaccg tcggaaggac
     1021 ctaaccaacg ccatccgcac ggcgctggga cgggcagaca tcgtaattac gatgggcggc
     1081 attggtccca cggaagatga cctgacgcgc gaagcctggg ccgacgctct ggaagtcaaa
     1141 ctcaagcgga atggtgatct tgtgggccag ctttacgcac gcgcagcgga gcggcgctgg
     1201 acgctgacgg aaaacaatct gaagcaggcc gacatgctgg aaggcgcaga ggtgcttgag
     1261 aatccgcatg ggagtgcgcc cgggcaatgg ctggacacgg tatttgcggg acatcgcaag
     1321 ctgggcatgt tgttgccggg gcctccgaga gaaatcaagc caatgtttga cgagcagtgt
     1381 atgccgcgcc tgcggagtat tctgccagta cgccacattg ctacgcgcac gctgaaggcc
     1441 gccatgatcg gcgagtcgct ttgcgattcg cgcatcgccc cgatttatac gcaatatccc
     1501 gacgtggaga cgacaattct agcgggcgcc ggggacattc agataacgct gatgtgccgc
     1561 cactccctgc cggaacgtgc ccaggcacgc gtagatgagc tggccagcag gattgaagaa
     1621 gagctggatg atttcgtcta ctcctcacag aacgaatcgc tggagcagat tgttctttat
     1681 tatctggaga tgcgtggcgc gaccctctcg gtcgcggaaa gctgcacggg aggaatgatg
     1741 gcgcagcggc tgaccagcat cagtggaagc tcgcgttcgt ttgtaggcgg cgctgtagtc
     1801 tacagcaacg agctgaagac agagtttgcc ggagtccagg aagagttgat tgacaggcgc
     1861 ggtgccgtga gcaaggaagt tgccgcagca ctggcggacg gcattcgacg gcgatgctca
     1921 agtacttatg gcgtgggaat tacaggcata gccggtccaa ccgggggaac agaagagaag
     1981 cctgtggggc tggtgtacat cgcgttaagc gatggcgcgc agactgaagt catcgagaag
     2041 atctttgcgg gcgaccgtga gcgcatccgt tattatgcca cccagcaggc actcgacatg
     2101 gtacgccgca agctgatgta gctgctacac tcagaatcgc aatgctagtc gttccatacc
     2161 ttgtaattgc gttgatctcg tatctgctgg gttctgtccc tttcggatat gtgttcgtgc
     2221 gggtctttcg taaggaagac attcgcgcga agggaagcgg caatattggc gctaccaatg
     2281 tcatccgttc cggcgcgaaa ggcctgggcg cgctcacatt tctgctggat gcgctgaagg
     2341 gtgcctttgc tgttctggcc gcgcggtgtt tcttcccttc tgttcctgcg cagaatgcag
     2401 cggcacttgc cgcattcttt gccattcttg gacatatctt tccggtgtgg ctgcgcttta
     2461 agggcggcaa aggggtggcg acggcgctgg gagtctttct tgcgcttgca tggattcccg
     2521 cactggccgg acttggggta ttcatcatcg tctttgcggc atttcgttat gtctctcttg
     2581 cctcgatttt tgccgcggca gcatttccgg tgtttgcgtt tctgttgcca catcagccgc
     2641 cctataacac gtggatgatt gccgtcatcc tgattgtgcc ggtcatcgtg attgcgaaac
     2701 accatcagaa catctaccgt ttgctgcacg gcactgaata tcgctttgga aaatcgaagg
     2761 aagccgctgc atgagccgca ttgctgtgat gggcagcggg gcatggggca ctgcgattgc
     2821 gctgagtctg gcgaagcgcg gcggacacga aattacgctc tggtcccatg cgccggaagt
     2881 gagcgaggcc attcgagagc gcggcgagaa ctctattttt ctgcctggat ttccagtgcc
     2941 cgggaacgtt cgcgctactt caggcattca agaggcgctg cgtggggcgg agatcatcat
     3001 cagcgtaatg ccgtcgcacc atgtgcgcgc aagttatgtg cagtttgcgc cgcatctcag
     3061 gcctgggcag gttcttgtga gtgccacaaa gggcgttgag gatgaatcgc tgctgcgcat
     3121 gagtgaagtg attactggtg tgctgcgcgg cttcgatttg cagttgccgc ttggggtcct
     3181 tagcggaccg tcgtttgcgc aggaagtagc ggcgggtttg ccggccgcgg ttacgattgc
     3241 ttcgcaggat gttgacctgg catcgcggat ccagcgcgag ttcgggagcc agacactgcg
     3301 cctttatacg aacgatgacg tggtgggcgt ggaactgggc ggggccttga agaacgtcat
     3361 tgcgattgcg gcaggaatcg ctgcagggct tggtcttgga cacaacagcg ttgccgcgct
     3421 cattacccgg ggtattgcag agacgacccg tcttgctgtt gcctgtggcg gccgacgcga
     3481 gacactggca ggactctcgg ggattggcga tctcgtgctt acctgcaccg gtgctctctc
     3541 gcgaaaccgt accgtcggca tcgaattagg aagaggccgc agacttccgg agatccttgc
     3601 gggactgcat ggcaaggttg ccgagggtgt gcgtacaaca tcagcggcgc tgggcctggc
     3661 acggcgctat ggagtagaga tgcccattac cgagcaggtt gccgcgatcc tgaatgaaga
     3721 aaaatcaccg cagaatgcga tgcgcgagct gatggcccgt cctgggcgcg acgaatagat
     3781 tgctacactt gaagcagcgg tattttccat gattcaaaca ctctttggca gcctgaaaga
     3841 ggctgaggaa ccggaaaaga aaagtatctt cgaccggatg aagcaggcgg tcacgcgcac
     3901 ccgcgaaagc ctgagcgaac ggatcgagag cgtcatggcg atgacgcgcg aggtagacga
     3961 atcggcgctt gaagagctgg agatgagttt gcttgcctca gatctaggtg tcacaacgac
     4021 gatggagatc cttgataacc tgcgcgagcg ggcaaagcgg cagcagatac gcgatggcgc
     4081 ggagctgaag tcgctgttga aggagcagat caagggcatt ctggatgagc agcaacgccc
     4141 ggcgccgttt gttcctgcgc ctcctgcggt catcatgatg gtgggtgtga atggcaccgg
     4201 caagacgacc accagcggca agctggctgc gttttttcgc gcgcaaggga aaacggtgtt
     4261 gctttgtgca gcggacactt ttcgcgcagc agcgatcgaa caactggagg tctggagcca
     4321 gcgcagcagt gtggaaatga ttaaaggccg tcaagggggg gatccgtccg cagtgctgtt
     4381 tgacgcattg caggctgcga aggcgcgagg aaatgatttt ctgattgtag acaccgctgg
     4441 ccgcctgcat acgaaaggcg gcctgatggc ggaactggac aagatgcgcc gcacgacgca
     4501 acgaattatt cccgatgctc cgcatgaagt gctgctggtg atggacgcga ccacggggca
     4561 gaatggattg cagcaggcgc gactgtttac agagtccgcg ggggtgacag gcatcgtgct
     4621 tacaaagctt gatggcacgg caaagggcgg catcgccgtt gcaattgcgc gggaactgaa
     4681 gctgcctgtg cgttatgtag gtgtgggaga gaagctcgaa gatattctgc ctttcgatag
     4741 cagcgctttt gtggattcgt tgctgggagc ctgattcaca tgcaagatga catccgctgg
     4801 atgaagcgtg cgcttgcctt ggcgcagcag tccgttgggg tgtcctcacc gaacccagcc
     4861 gtggggtgcg ttcttgtgca gagtgatggg agcgtggtgg gggaaggctt tcataaatat
     4921 gaggaattcg accacgcgga aattgttgct ctgaaacaag ccggcgaaaa agcgcgtgga
     4981 gcaactgctt acgtaaccct ggagccctgt tctcatcgtg gacggactgg gccgtgcgcc
     5041 gatgcattga tccacgctgg tattgcgcga gtcgttgcag cgacgggaga cccgaatccg
     5101 gcggtgaatg gacagggaat cgaaaagttg cgtaaggccg gtattcctgt agatgttgga
     5161 gtgctttgcg atgaggcccg cgcaatcaat gatggttttg cgaagtacat ccgcaccggg
     5221 ctgccgttcg tcacgctcaa ggccggcgtc tcgcttgatg gccgtattgc gcccgcgccg
     5281 ggaagccaga aagccgggaa gccgtattac atcaccagcg aaatgtcgcg ctttcaggtc
     5341 cagcagatgc gccatgcctg cgatgcgatc attacaggca ttgggacggt gcttgcagac
     5401 gatccgcaat tgacagatcg cagcggatgc ctgcgccgtc agccgctgct tcgcgtggta
     5461 ctcgattcat cgctgcggct cttactcaat tcaaagctgg tacagacggc gaaggaggat
     5521 gtgctggtgt tttgcacatc tgctgcgcct gctcaaaaac agcgcgaact ggaggcacgc
     5581 ggcgttcgtg ttgaacaagt tcctgaatgc gaaagacgtg tttctttgga gcacgtcctc
     5641 agacgattgg gagagttgca ggtattaagc gccatgctgg aaggtggagc acagctaaac
     5701 accgcggcca tgcaagcaca gatcgcagac aagctctgct tcttttatgc tccgcagctt
     5761 ttaggcgaga atgcggttcc cttgtttgca ggaagtgcga gaattcaacc ggagatgcag
     5821 cgcatctcgt ggaaagcgat aggaaatgat gcgctgtttg aggcatatct gcgcgatccg
     5881 tggaagtagg agacatttat gtttaccgga ctgattgaag cgacgggaaa aattctcagt
     5941 atcgaagagc gcgcgggtac gaagcgcatt gcggtttctg cgccgggaat tgccgggcgt
     6001 ctgcgcacag gtgattcggt ggcagtaagt ggcacgtgcc tgacggcgct cgatatttct
     6061 gaagagccac ccgtgtttca cgctgacctt gcggcagaga ctgtagagcg gacttctctt
     6121 gcaaggttga aaccaggggc ggttgtgaat cttgaattgc ctacgccttc ggggactccg
     6181 ctgggtgggc atgtggtgca ggggcatgtg gacgcgaccg ggacgctgct tgcgctggaa
     6241 ccggtttctc ccggcggcga tccagcggca acagactggt ggctgcgtat ttctgttccg
     6301 gaaggcgtgg cccggtatgt ggttgaaaaa ggctcgattg cgattgaggg cattagtctt
     6361 accgttgcaa agttcgatgg caggacgctg accatcgcca tcattccaca cacctatgcg
     6421 catacaaatc tgcatacgct tcagccaggc gaccctgtga acctggaggc tgacgtgatg
     6481 ctaaagttct ttgagcagcg gcggcagcag gaggaagaag ccgggtttga gctgacgctc
     6541 gaatatctga ttgcaaacgg gtactgacag ccagacgttg ttgcattctt tctttgtttt
     6601 agtccggtct ttctttccag tgtagactca agacgtgcag gtgcagaggc cctggtggcc
     6661 cctgttaaac gggaagccgg tgggaatccg gcactgtccc gcagcggtga tgggagcgaa
     6721 aggcgcatgg ctgcgaaagc caggcactgt tcaatgatga atgggaagcc gcgctgagta
     6781 gatggcccaa agtccgaata ccggcctgtg caagcgccat cctgtgcgta tactcgtcag
     6841 acggcctcgg ggaatgggcc ttgctgacct tggtccctgc gcgcagatgg ttccattttt
     6901 tgaccggaaa gggaaccact ctttatgtct ctgaaaactg caaatctggg ctattcgcgc
     6961 atgggagcgc accgtgagct gaagtttgcg ctcgagtctc actggcaagg taaaagcgat
     7021 gaagaggcct tacttcatgt cgcagatacc ctgaagcaca atcattggtc cacacagcag
     7081 caagcgggaa tccagttcat cccatgcaat gacttttctc tttacgatca tgtgctggac
     7141 atgctggttg cgctgggggc cacacctgtt cgctttggcg atggcgaggt gacgctgcaa
     7201 cgctatttcg atatggcgcg taacagtcag caacaacctc ccatggaaat gacgaaatgg
     7261 ctggatacaa attatcacta tctggtcccc gaatggtcga acaaccttac ctggaaaacg
     7321 gacccggcaa agctgctgca tgagctggcg gcaggcaggg aacatgagct gcatctccgg
     7381 cctgtgctga ttgggcctct ctctcttttg ttgctgggca aatctgtaga tggtacagac
     7441 ccgctcaaac tgctgcctcc aatgctggaa agttatgcat cgttgcttgg ttcccttcac
     7501 gctgcgggag tggactggat ccaggtagat gaaccttatc ttgtaacgga cctcacacct
     7561 gaatatcagc aggcctatcg gaccgcgtat gagtatcttg ctgaaaaacc actgaagatc
     7621 atgctgacca cgtactttgg cggtctggga gaaaatctgg acctgacatt ttctctgccg
     7681 gtagacgggg tgcatctgga cctggtgcgt gcgccggaac aactcccaac ggcattagaa
     7741 gccatacaga gtgagcaggt gctcggtctg ggctgtgtga atggacgcaa tatctgggcc
     7801 gcagatctgg acaaggccca tgctctggcg caacgcgcgg tccacgctct gggtgaagag
     7861 cgcgttgtgg ttgcaccatc atgctctctg ttgcatgtac ctgtagatgt ggacgtggaa
     7921 gatgcgctac cggatggaat cagaccgtgg ctgagttttg ccaggcagaa actcgaggag
     7981 gtgcgatgga ttgccggtgg tccacagatg tttcctgaag tttttctgga gcggcgccgc
     8041 attttgcagg aacgcgccca ggctgcttcg gcccatgtac ccgctgtgcg caaagcgatc
     8101 gctgacttaa cagaaaggga ttttcatcgt gctgccgcat accgtgagcg tgtcgcaacg
     8161 caacgcgccg ctcttgggct gccgcttttt ccaacgacca ccatcggatc ttttccgcaa
     8221 acagcggaga tacgcaaggt cagagcgaag tggcgcaagg gcgagatgac gacggccgaa
     8281 tacgaggcct accttgaaaa cgaaatggct gcatgtatcc atactcagga agaaatcgga
     8341 ttggacgtgc ttgtacatgg agagttcgag cgcaacgata tggtggagta tttcggagag
     8401 cagcttgcgg gctttgcatt tacaaaaaat ggatgggtgc aaagttatgg atcgcgctgt
     8461 gtcaagcctc ccatcatcta tggggacgtg ttccggccag ggccgatgac agtgcgctgg
     8521 agcgcaaagg cccaatcact cacgcagaga cctatgaaag gcatgcttac cgggcctgtc
     8581 accattttgc aatggtcgtt tgtgcgggac gatacttcgc gcagagaggt cgccttccag
     8641 atcgctctgg cgctgcgtaa ggaagtacag gacctcgaaa cggcacagat acgtgtcatt
     8701 cagatagatg aacccgcatt gcgggaaggg ctgcctctgc aacgcgccgc ctggaaagaa
     8761 tatcttgagt gggctgccgc ggccttccgg ctggcaacat ccagcgtaaa agatgccaca
     8821 cagatccata cgcacatgtg ttacagcgag ttcaacgata tctttccagc gatcgcagca
     8881 cttgatgccg atgtcatctc cattgaatcc gcccgctccc gtatggaact gctccaggcc
     8941 ttcagggagt cgggttatcc taacgagatt ggcccgggtg tctacgacat tcatgccacg
     9001 cgtgtcccgg acaaggctga aatcgaagaa ttgctgcaaa gggcgcttga agttgtgtcg
     9061 ccagaacagc tttgggtcaa tccggattgc ggcctgaaga cccgcaactg gcctgaggtc
     9121 atagcggcgc tcaaaaatat gtgtgccgcg gcgcgcgaat tgcgccaaca atatgctgta
     9181 ctctcatcgc gataaatgct cttttccctg tcgcctcccg gagcgcggca gggagaatgg
     9241 cccatgccat cgctaagcgg tagactgaat cctgatgtct tctgcgaaca ccaccctagc
     9301 cccggtcttt ctgctcgatt ccatgtcgtt tatcttccgc gcataccatg cgatgcagcg
     9361 gcagaggccc atgtccacgc gtacgggagt gccgaccgca gcaacttacg tcttcgtcaa
     9421 tatgctgaac aagctgcggc gtgacttcca tcctgaatat ctggcagcga tttttgaccc
     9481 cagcactcca gtctttcgtg atgagcgcgc gaagacgatg acgaccatcc gcaagttcaa
     9541 tataaaaacg cagacatttg atgaagtggc ctatgcagga tacaaggcga cccgcacaga
     9601 aacaccgcct gacctcttac agcagctacc gtatatccgg cgagcactgg aggcgtttca
     9661 cattcctatt ctgctggccg agggttttga agccgatgat gtgataggta cgctggcgaa
     9721 caaggccgca aaaaaggggc atcccgtatt tattgtctcg aatgataagg acatgctcca
     9781 gcttgtaaac gatcatgtaa aggtgctcaa tccggtgaag gacaatcttg ttcttgaccg
     9841 cgctaaagtc gaggagacgc taggtgtgcc tccagagaag gtaattgatg tgatggcgct
     9901 gcgcggagac acgattgaca atgttcctgg tgcgccgggg atcggtgaca aaggttcggt
     9961 ggagctgatt cagcagttcg gcagcctgga agcagcgctg gaccgggcat cagaggtgaa
    10021 gcgcaagacg tatcgtgaat cgcttgaaaa taaccgcgag acaattttgc taagtaagga
    10081 actggtaacg atccattgcg aggtgccgat tgaactgaat ctggatgaga tgcgcacgca
    10141 gccgccggat gtgaatgcgt gccgccagct cttcagtgaa ctggaattta cgacgctgct
    10201 gaaggagctt gctccggctg cccaggagaa ggaagtcgag cacattcttg aacccacaaa
    10261 ggagcagatt gcagaattta ttgcgatggc gcgtgcgcag ggccttgctc tggcggtccc
    10321 tgaatctgaa cttgcggcca ttgccgagca ggtgagcgag atggaggccg aatctgccga
    10381 ggaacaagag ccggaattaa agaccatgtc tctgctggac attgcggagc aagcagagag
    10441 accggatcca ggaaaacagg tgtgcagcat tggcgttgcc gcggagcccg accgcgcgct
    10501 gctggtcccg ctcgatgcag tgaaagcgtt gctggaagac gcttcggttt ccaaacatgt
    10561 gcatgacctg aagggcacga tgcgcgccct tgaaaaccac ggtgtggaat tacgcggtgc
    10621 acaggatgac ctgatgctct actcatatct ggttaatcct acacatacct cacaccggct
    10681 tagcgatatt gctgcgcgat tcagcaatca tcccttgcag gaaactggcg agaagcagct
    10741 tacggaagcg gtccatgcga ttcatgcgtt gactccgatg ctgcgtgaag aagtggacac
    10801 tctcaggacg cgtgaagtct acgagacgat tgatctgcca ctggcgccgg tccttttgcg
    10861 aatggaaaaa gctggagtgc ggattgattc tggtgttctg caagagattg gagagcggct
    10921 ttcggttgag atccatcgca ttggcgaaga gatttatcag cggtcgggac atcgcttcaa
    10981 tatcaattcg ccaaagcagc ttggcgatgt gctctttaat aaaatggggc ttcccaagcc
    11041 tctgaaatat ggaaagggga aggtcgtttc tacagcgcag gacgtgctgg aagaacttgc
    11101 agagcacaac gaagtgccgc gcatggtgct ggaataccgt cagcttacga agctcaaatc
    11161 gaattacgta gatacgctgc cattgctggc cgatagcgaa ggccgcgtgc ataccacgtt
    11221 taatcaggtg ggcacggcga ctgggcggct ttcttcaaca aacccgaatt tgcagaacat
    11281 tcctgtcaag actgagcttg ggcgcgagat acgcgctgca tttattgctg ctcccgggcg
    11341 cgtgctgatt tctgctgact attcgcagat tgagcttcgg ttgatggcgc atttttctga
    11401 agacccattg ttggtgaagg cgtaccagac cgggcaggac atccatacac tgacggcatc
    11461 tgaagtcttt agtgtccctg tagacaagat gaccaaggac atacgcaatc gcgccaaggc
    11521 ggtgaacttc gggattgtgt atggtatttc tccctttgga ttggcgcagc agcttggaat
    11581 tgatcagcat gaggcgcggc tttatattga gacctacttt gcgcgctatc agggggtgcg
    11641 ggcctacatt gaccgcgtgc tcgaagatgt gcggcgcgag cagcgtgttc gcacactctt
    11701 tgggcgggtg cggcctattc cagacattca gagccggaac gccaatctgc gcggcttcgc
    11761 agagcgcacg gcgattaaca ctcctttgca ggggacggct gccgatttga tcaagctggc
    11821 aatgatccgc atagaacgca aacttacaga gaagaagctg aaggcagtca tgacgcttca
    11881 ggtgcacgat gagcttctct ttgatgttcc ggaagatgaa gctgatgggg tactcgcatt
    11941 ggtgaaacag gagatggagc acgtcattga gctgaaagtt ccggttgttg cagagtgtgg
    12001 cgttggattg aactggagag acctgaagtg agattgtcct ggctggtcaa aatcttaaca
    12061 gtattgatta aacccttaca tcaccaagat tcttcctgtc tgcggtcgct atagacttac
    12121 gggtactgtc agacttggtc tgatgcgtat ctgtgataca taaacagaga gcgaggaaga
    12181 tttttggatt atcacgccta ctatgctgtt ggcagcagcg tcctcaacag caggaacaat
    12241 aaaacgccag cgaagaaaac cagtgccatg cgaatgcctg gttcgcgatt gacctccgga
    12301 accagatcgg tcgctgcgac gtagatggcg actccagcag aaagtggcag gccagcttct
    12361 acccaggaag gaaggacatt gatgagggcc acgcccagca gcgtggcggt tgccaataca
    12421 gcggcggcaa caacggcagt gctgcggctg cgtcctgacg ccatcatgac ggaggcgacg
    12481 gtgaatccct ccgggacctt gtgcagaaaa atggcaataa agatcaacca ccccagcgtg
    12541 gcggacagta caaaaccaga ggcaatcgca atgccatcaa agagtgcatg cacggccagt
    12601 ccggcgagaa ccgagtagct ggtgcgggcg gaaagaaact cgtcgtgatg ggtctcttca
    12661 ccaaaatgga agtgtgggac gatggtgtgt tccagcagat ggacgatgca atagccaaca
    12721 agcaccaata acggggccca tcgcactgag aagttcatgc tttcaggaag catttcaaga
    12781 aatgctacag caagcatgaa tccggcccct aacgccacga aatagcgaag atagcgcttt
    12841 tcccaattgg agcgcaccag gaccaggccg ccgaaaatat cggcaactgc ggcaaaggaa
    12901 ccgagaatga acgaaagcag tctcatgctt aataaaatgg caattcttca tcatacctgt
    12961 tgcaaagccc agtgccgtct cgcgcgggga gttgagaaga aaagcccata gcagagacac
    13021 tttttgcgga gtagcatcag tctccaaatc gcacggccat tccccgcgtg aggtgcaata
    13081 cccatgcccg aaatcaagcg gttcctgaaa aatgaaagaa aacatctgtt gcacgtagcg
    13141 tttgaacttg gtcagcgttg caaggtggac atcctgcccc ggcacttcta ttcagagata
    13201 ccggacattc acgacctgaa aaactccagg caatggagag cgccccgaag tttttacggc
    13261 attccgtgcg atatggatgc acaggtggac tgggtagagc agtgcaccag gaatttccgg
    13321 gacaagcttc agaacttcga tctccataag agagcattgc agttgaatgg catggataca
    13381 ggtttcgggc aagtcgaagc ggattttctg tactgttttg tgcgtcgcat gcggccagcc
    13441 cgtattcttc aggtaggttg cggggtctcc actgcggtct gtctgtttgc cgcggaggac
    13501 gaaggctata cccccgaaat catatgcatc gaaccttatc ctgacgaatt tttgaagggc
    13561 caggcacagg ccgggcgtat acaactggta gataaaaagt tgcaggacgt gggctttatg
    13621 tgcgccgagt ggctgtcagc gggggatttt ttctttgttg attccagcca caccctggcg
    13681 ccggccgggg aagtgaacgt cattgtcctt gaaatgttgc ccaggcttgc gaaaggcgta
    13741 tatatccatt ttcacgacat atattttccg tatgattatt cgccggttac gctttccgga
    13801 gcgctgtttt tcccgcacga aacggcattg ctgtatgcgt tccttttgat gaatccaggg
    13861 tttgagatga tggcgtcact ggctatgctg caccacctgc gccggaacga cctgctacgg
    13921 ttcttcccgg agatgaagcc aaaagaattc accgatgggc ttggaattac agacgggcat
    13981 ttcccctgtt ctatttatct gcaccgctgt gcttagagcg ttttcgactc acttgttcac
    14041 atctctggca tcctgcattc agtagacagc agaatcgaaa acgctgtagg tggccagcag
    14101 tgcagaacgt tttaactgct gactcctgaa cctgccagct ctgcgacgcg cgtgtggacg
    14161 atgcgtcctt catgcgtaac cagagcttcg cggatgattt catcctcaag attgagcgtg
    14221 atttttcctt cattcatcag cagctttgct actgcagcta cgttagcaga aaacatctgg
    14281 ctggcatgat taggaatgct tgccggcagg ttgaccgggc cgagaatcgt cacatcgttg
    14341 tggacgacgg tttcgccggc ctttgtcatc tcgcagttgc cgccgcgttc ggcagcaata
    14401 tccacgatga cagagccggg ctgcatggca gagaccattt cagcggtaat gagcacgggc
    14461 gacttgcgtc cggggacagc ggcggtagtg atgacgacgt cctgttcgcg cagcacttcg
    14521 gtgagttgct cgcgctgctt gcggtagaag tcttcgccga gggccttggc gtatccactt
    14581 ttatcttccg ccgcctgcgc ttccacgtcc aggacgacaa atttagcgcc gacgctttca
    14641 acctgttcct tgacggctgc gcgcacgtca taggcgctta caacagcgcc gaggcggcgg
    14701 gcggtagcga tggcctggag tccggctacg cctgcaccga ggatgaaaac ttttgcggga
    14761 gtgatggtcc cggcggcagt cataagaaga ggaaacatct tgggcaagtg tgttgccgcg
    14821 agcaggacag cgcggtatcc ggcaatggtg gccatcgagg agagcgcgtc catgctttgc
    14881 gcgcgagtga tgcgcgggat gagttcaagg gccaacagag agactccagc ggatgccaga
    14941 tcggagcatt cttggatggc agtcagcggc tcagcaaagc cgatgagcac ttgtccgggg
    15001 cgaaggtgcc tgaggtcggc gcggccagct tcaggattgg cgccgagaca gcgcacctgg
    15061 gcaatgatgt ccgcctgctg aaaaacatct tcccggttgg cgacgcgcgc gccacgggcg
    15121 atgtacagct cgtcgggaaa gccggcagcg gcaccggcgg attgttcaag gatgacctca
    15181 agtccggcct tcttcagcgc ctcgcattga cggggtacga gtgctacgcg gcgttcctga
    15241 ggataggtct cacgcagaat cccaagggtt ccaggcaatc gcaaaaatct ccttggggag
    15301 ccaagttatt caatttgttt atggtttggc aagaggtttc gttgatgaga ttagaaaaat
    15361 ctttgtcgtt catagagact tgtggcccat gggagtaaag tccagcggcg tcaagtcagc
    15421 cgggagatga caaaattcaa gatggaaggc aacgggcgct cactcaaaac cgaagctttc
    15481 tgtggatggt ttccgtttta taacgttcac gatgcattaa ggttacggaa cttaacttat
    15541 aggtgatgct cttgagtcgc agggtaggaa aagtagttct ggcgatcgcg tctctgtctg
    15601 cgtgtggtgt ttatgcccag cagacggtca cgatttctct ggatgaagct attcgtcgcg
    15661 ctgaagcaaa tgagccggct ttcgtggcga gtatggcaga cagcaaagtg gctgcgctcg
    15721 accgcgggat tgcgcgttcg gcactcctgc cgaatgttgt gtaccacaat cagtatctat
    15781 atacgcaggg cggcacggga agcacgacgc cgcgatttat tgcgaacaac gctgtccacg
    15841 agtatgtgag tcagggggca gtgaatgaaa ctctgggact ggccggaatg gctgaggtaa
    15901 aacgtgccga tgctgctgct gcgtttgccg cggcgcagtt ggaagtggcc cggcgcgggc
    15961 tggtggccac agtggtcaat ctgtactaca gccttcttgc ggcagataag aaggtggcag
    16021 tggcggagcg cgcggcaggt gaagccgatt cgttcgttga tctgacgcaa aaacgtgaaa
    16081 cggcgcggga agcggcccat gccgatgtta tcaaggcgca actggagcag cagcagcgtc
    16141 accgtgatct ggaagacgcg aaggttgcag cggaaaaggc gcggctggaa ttaggtgttc
    16201 tgctctttcc tgatccgcgg acgccatatg ttgaagaaag tgcctcgatt cgaaaaaacc
    16261 tccccacacg ggcggaagtg gaaacggcgg cctcggcgaa caatcctgat atcaggagcg
    16321 cattggccgc gctgcggcaa agcgatgcag aagtgctggc ggcgcgcgcg gcctatctgc
    16381 ctgatttggg gttgaatttc acttatggca ttgatgctcc gcaatttgcg gcgaacgggc
    16441 cggaccatgt gcgcaatctt ggctattcgg cggctgtaac actggacatt ccggtgtggg
    16501 actggctttc gacgcagcac aaagtgaagc aaagcgagat ccgacgcaat gcagcgcgtg
    16561 tggcattgac gtcggcacag cggcggctcg tggcggaact cgacgaattc tattcagaag
    16621 cccaggccgc gcacaatcag cttgcttcac tcgatgcaag cgtgcagacg gccacggaaa
    16681 gcctgcgcct aacgaagctg cgctacacgg cgggcgaggc cacagtgctg gaagtagtag
    16741 acgcgcagaa tgccctgacg ctggcggaaa atgcgcggga ggacgggcgc gtccgttatg
    16801 agctggcgct tgcgaattta cagacattga caggaacact gtaagagaac gaaatgctga
    16861 acattacagg aaaaatctat ggctctgctg cgttggctct tggcctgatg ctcgcaaccg
    16921 gatgcggaaa aaaagaaaca gcgccgcagg tcgaggttga ggtgcaggca cagcatcctg
    16981 aggtgggacc gatggcagcg catatcaccg cggatgcggt gctggccccg ctggcacagg
    17041 cggcgattgc cccgaagatc agcgcgccgg tgaagaagtt ttatgtgcag cgcggatcgc
    17101 atgtgaaggc cggccagttg ctggctgttc tggaaaaccg tgatcttgaa gccgctgcgc
    17161 tcgacaatca gggcagctac acggccgcgg aagcggccta tgcaacggcg acaaaggcgc
    17221 aggtcccgga ggactaccag aaagcccagc tcgattttgc gcaggccaag gcaaatcttg
    17281 agttgaacga gagcatcgta aaggcgcgca agcagctctt tgccgaagga gcgattcccg
    17341 gccgcgatct ggataccgcg gaggccacgc ttgttcaggc acaggccgcg tatgactcgg
    17401 cgaagaagca cctggattcc atgcagaccg taagccgcga ggccgcgctg aaagccgcgc
    17461 agggacaact ggaatctgca aagggaaaat atctcggcgc agaggcgcag gtcagctatt
    17521 cagagattcg cagccctatc catggcgtcg ttacagaccg gccgcttttc gcgggcgaga
    17581 ctgcggcggc gggagctcca ttgcttacgg tgatggacac ctctgcgctg ctggcgaagc
    17641 tgcacatctc gcaattgctg gcgcagcagc taaggattgg tgattccgca acagtcacag
    17701 tgcctggaat ggaggagccc gtccctgcga aggtgtcact catcagccct gcgcttgatc
    17761 ccggcagtac gaccatcgaa gtatgggtga agaccgagaa caaagatggc gcgttgaaag
    17821 tgggcactcc tgttcatgtc gccatccaga cgaagactgt tcctgacgcc gtaaaagtcc
    17881 cggcaagcgc gctgctgact gcgcaggatg gcggtacttc cgtgatggtg atgggggccg
    17941 atggagtagc gcataaaaag cctgttcagg tggggatccg agatggagaa gatgtacaaa
    18001 tcctcaatgg catcacaccc gcagacatgg tgattaccga aggcgcctat ggtcttgacg
    18061 atggaacgaa agtgaaactg gccgctgccg ctgccaaaaa tggggacgag aagtgattac
    18121 cagtcctccc ataacgcagc cctctacaca gagcttctgg cttgcgcgct cggtgcgcac
    18181 ggtcctgttt tttgccattg tgctgactgt tgcggggatc tatctcgcct ttcaggtgcc
    18241 tatttctgtt tttcctgaga caaattttcc gcgtgtcgta attggcgtgg acaacggcgt
    18301 gatgccggtt gaacagatgc aggtgaccat caccaaaccc attgaagacg cggtgaacag
    18361 cgttcccgga ctgctgacgg tgcgctcgac tacgagccgc gggtcggctg aggtgagcct
    18421 tttctttaac tggaatgtgg acatgttcca cacgctgcaa ctggtagatg cagctttggc
    18481 caaagtcgag caaacattgc caccgacggc gcgcattacc accaaccgtc tgacctttgc
    18541 cacgtttccg atacttggct acagtctgac ttccgacacg ctttcgcaga cgcagctctg
    18601 ggagctggcg acgtatgaat tgaagcctcc gctgaaccgc gtggccggag taagcacggt
    18661 cacggtgcag ggcggaaagg tcccggaata tcacattgtt ccggacatgg cgcggatgca
    18721 ggcatcaggc atcaccatta ctgatcttgt gaatgccgtc caggcgtcga acattattga
    18781 ttcgccaggg ttatatgaag aaaatcacca attgattctg gggcttgtag gcgcgcaggt
    18841 gcatagcgcg gcagagttgg gccagcttgt cattaagaca acaccgggag gcgcgcctgt
    18901 tcgcgtagta gatgttgcgg aagtgaagcc agctacgatg ccggtctata ccgtcgtaac
    18961 ggcgaacggc aaaccggctg tcttgctgaa cattacacgg cagccttcaa gcaatactgt
    19021 agccgtggca aatgcagtgg ctgatgaggt gcaacagctt cagaagaaac ttccgcccgg
    19081 tgtgaagctg gcgccgtttt atgaccagtc ggagctggtg cgcgaaagca tttccagtgt
    19141 gcgcgacgca attctgatcg ggctggtgct ggcgtgcgtc atactctttt tgtttttgcg
    19201 agactggact tcttccatca tcgcgggact ggtcattcct gtaactgttg cggttacaat
    19261 tctttttctc tggatgatcg gcgagagctt caacctgatg acactgggcg gtcttgccgc
    19321 ggccatcggg ttggtcattg atgatgccat tgtagtggta gaaaacattg tcctgcatcg
    19381 cgattcaggt gagacgcgca ttgaagctgt gcgcaaggcg ttctctgaga ttacaaagcc
    19441 gttgatcggt tccacgatta ctccggtcgt tgtctttctt ccgcttattt ccgtctctgg
    19501 agtcacaggc agcttttttc gtgcgcttgc tgtgaccatg acggccgcgc tgctgacctc
    19561 acttttgctg gcacttacat ggacccctgg acttggcctt gttctgctgc gcgagcggca
    19621 tgatgttcat catgccgggc cgggaaaggt gttgcgccgc gcgatgggtt ggcacagccg
    19681 cctgctggaa tggtcgcttg caaagccgct gttgcttgct gttgcctgcg cagttcttgt
    19741 tctcggaacg tggttcgcct atcaagcgct cggtacggac atgcttccag aaatggacga
    19801 aggcgggttt gtgcttgact acataatgcc tgcgggcagt tcacttacag aaacaaaccg
    19861 cgtgttggag catgtggagc gcatcctgca atctacacct gaagtagaga gcacatcgcg
    19921 ccggactggt ctgcaaatgg gattggccgc ggtaacggaa gcaaacaccg gagacatcac
    19981 cgtaaagctt aaaaaagacc gggaccgcgg aattgatgaa gtgatgaacg acatccgcga
    20041 gaagatcaag acgactgaac ctgaactcga cgttgaattt acgcaggtct tgcaggacat
    20101 gatcggcgat ttgtcgaatg cgccggagcc gatccagatc aagattttcg ccaatgatca
    20161 gaaactgctt aatgagcttg gcgaacgcgt ggccgacaca atcggtaaga ttccgggcgt
    20221 ggtggatgtc caaaacggcg tagagaacac catcagtggt cctgccaccg gctttcaggt
    20281 ggacccaacc gtagccgcgc ggcttggatt tacgccgcag gaagtgtcgc aggacgcgac
    20341 gagtattctc gatggaattg cgacagcaaa tccgctgatt gcgaacaatc gcccatatac
    20401 gattcgggtg cgtctgggtg atgcatcacg cacgtcgctt gaagcgattg agaatacggt
    20461 cttcaacagc agcagcgggc atacggccac gttaggatcg ctggcgcaga ttcagcagtt
    20521 gcctccacag aatgaaatcc gaagagagaa ccttcagcag atgattgtgg tcacagggcg
    20581 gctggaaggt tcagacctgg ggacggcgat ggcgcaggtg cagcgcgcgg tcgaaaatct
    20641 tcatttgcct tcgaatgttc gtgtaaccta cggcggaaca ta
//
LOCUS       DASRBV010000304         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_482572, whole genome shotgun sequence.
ACCESSION   DASRBV010000304 DASRBV010000000
VERSION     DASRBV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482572"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..502
                     /locus_tag="VFA02_11265"
     CDS             113..502
                     /locus_tag="VFA02_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZU10469.1"
                     /translation="MPTAQRFPQRGEIWWTDFHTDPPEKGRRPVIIVSPNARNQHERA
                     TSVLVIPLSTSVHKLGPAHLLLHAGETGLRMDCSAQADNIAVVLRAHLREPEPGQRPL
                     TNTKICKLASLVKLAMGCPEPMEAGEK"
     gene            complement(728..1471)
                     /locus_tag="VFA02_11270"
     CDS             complement(728..1471)
                     /locus_tag="VFA02_11270"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZU10470.1"
                     /translation="MPYRLMCITAHPDDESGAFGGALLMAHDQGIETSVVCLTEGSAG
                     SFRGEGVSDEELARIRRAELEAASRVLGITHTELLDYPDGALDRQDFSEIVAVLVARM
                     RARRPHVVLTFGGDGGVNLHRDHTVVSLVTTAAFHWAGRAKLFPEAGAPYAPQKLYYS
                     SPPVISVFNSNEGADTARVPYSLTLELGRWKEKKIEAFRQHVTQHGVLARVGSLIDKA
                     FGYERYLLAALRNGSLAADSSLFDGVVGD"
     gene            1547..2584
                     /locus_tag="VFA02_11275"
     CDS             1547..2584
                     /locus_tag="VFA02_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HZU10471.1"
                     /translation="MKKFALFLTVFLFCSLLHAQQPKALLYLTRSADGVRSFYAHADK
                     IDILVPTWYDVDGDGLVSGGPDPAVLAAAKQHHIEVMPIVASSGFQQAPFHKLITTPA
                     AWSRMVDQLIAACRQNGYSGFQFDFENIAWTDRDTLSQAVAKAAEALHREGLKLSIAT
                     VPNAPGAPGQSAFSAWLYANWRGAYDLSALAQSVDLICLMTYDQHTRWTAPGPVGGWG
                     WTTENLDYALKVVPKEKLSLGIALYGYHWFAGPPQNDKPNPSAEYIGGQDALDLARAY
                     GGKIEWDPVDRTAWTWFYRDDMREWIFFTDEHTFRERYDLVKQRGLQGFCSWVLGQED
                     PSIWNVLPSHK"
     gene            2619..3593
                     /locus_tag="VFA02_11280"
     CDS             2619..3593
                     /locus_tag="VFA02_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU10472.1"
                     /translation="MPTATQFETRQLGNSDLYLTPIGYGAWAIGGGNWEFAWGAQDDN
                     ESIQTIERAIESGINWIDTAAVYGLGHSEEVVAQALKRVSRKPYIFTKCSMRWDADRK
                     IYRSLKAASLQEELDNSLRRLQVETIDLYQIHWPNPEGEIQEGWETLGKFKKQGKVRY
                     IGVSNFNVEQMKRVQKIAPITSLQPPYSLLNRNIEAEILPFCQKNNIGVINYSPMVSG
                     LLTGKMTRERIASLPADDWRKRSPNFNEPKLSRNLKLVELLREIGKEHNVEPGVIAIA
                     WTLRHPAITAAIVGARRPDQIDGILPAAAFRLRDDEIARIEDFFHANP"
     gene            3641..3904
                     /locus_tag="VFA02_11285"
     CDS             3641..3904
                     /locus_tag="VFA02_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10473.1"
                     /translation="MSIFHDRRDELEKYEFMMGTTRGRLAVSLDALTDALILIGQHGV
                     YCTSNRNPSRPALDLETVMGEINGAKELIQSVMQDLEQIRNQQ"
     gene            3967..4713
                     /gene="fabG"
                     /locus_tag="VFA02_11290"
     CDS             3967..4713
                     /gene="fabG"
                     /locus_tag="VFA02_11290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZU10474.1"
                     /translation="MPSLEGKTALVTGASQGIGRACAIALARAGASVALAARNESKLN
                     EVAAEITANGGAAKAFVLNVASEESIKTTAKAVLDHFGTVHILINNAGITKDTLLLRM
                     KRSDWDDVINTNLTGTFLLTQALIGSMMKARWGRIINISSVVGETGQAGQANYAASKA
                     GLIGFTKSLARELASRNITANAVAPGYIETAMTAVLDEKQRVAMLSQVPLGRAGTDED
                     IANAVRFLASDEAAYITGHVLDVNGGMFMG"
     gene            4881..>5590
                     /locus_tag="VFA02_11295"
     CDS             4881..>5590
                     /locus_tag="VFA02_11295"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HZU10475.1"
                     /translation="MNRFVLAAAVFSLSGLSLVMAAQTATKPAVLVELFTSEGCSSCP
                     PADNLLAALDREKGYHGIPVVVLSEHVDYWDHEGWRDPFSSPDWTQRQNNYNERFHLQ
                     SVYTPQMVIDGTQQVNGSDGPAIAHALEKAAVRAQHVSIEIKDAAWSGSELQATVSIP
                     ETDAKGADIYAVLADDQDMSDVKRGENAGHTLTNVAVARVLHKVGKLRGPYNGQIRIS
                     LPSGMERGKMRLIVFAQKG"
BASE COUNT         1297 a         1580 c         1542 g         1171 t
ORIGIN      
        1 ttgaagctgc ctacgcggat tactacgatt ctcttagtga ggacgaagta gctgaggaga
       61 gcgcatgggg aagattcgca gagtcgcaac tcgcaaagga agcgcgctaa ccatgccgac
      121 ggcacagcgg tttccgcagc gcggtgagat ctggtggaca gatttccata ccgatccgcc
      181 agagaaggga cgccggcccg tgattatcgt ctcgcccaat gcgcggaatc aacatgagcg
      241 tgccacctcg gttcttgtga ttccgctctc gacatcggtt cacaagctag gtcctgctca
      301 tcttttgctt catgctggag aaactggact gcgcatggat tgttccgccc aggcggacaa
      361 tatcgctgta gtccttcgcg cacacctaag ggaaccggag ccagggcagc gtccgctcac
      421 caacacaaag atttgcaaac tggcttcgct cgtcaagctg gcgatgggat gtcctgagcc
      481 catggaagca ggagaaaagt aataggtctg tttcgacgcg gcggtgtctg gtggtatgag
      541 ttctacttcg cgggtcagcg catccgcgag tccaccaaat ccacgtcgaa aactcccgct
      601 gccgtcttcc gcccgtactt ctcaaagcct gtctgcgatg ccaatgttgg ctgccgcatg
      661 cgctctcggc ctccacgtac aaacttatcc aatcagactg tgaatcgcac agtttttcag
      721 aggttcgtta atcccctacg accccgtcaa acagcgatga gtccgcagca agagatccgt
      781 tccgcaaagc tgccagcaga taccgctcgt aaccaaaggc tttgtctatc aagctgccca
      841 cgcgcgccag cacgccatgc tgagttacgt gctggcggaa tgcttctatc tttttctcct
      901 tccatctgcc aagctccagc gtgagcgaat acggcacacg cgccgtatcg gcgccttcat
      961 tggaattgaa aacagaaatg acaggcggac tggaatagta cagtttttgc ggagcgtacg
     1021 gagcgcctgc ttcgggaaag agctttgccc gccccgccca gtgaaaggcc gcagtagtaa
     1081 caagcgagac caccgtatgg tcgcggtgta ggttcacgcc gccatctccg ccaaaggtaa
     1141 gcactacatg agggcggcgc gcgcgcattc gtgcaacaag cacagccacg atctcggaaa
     1201 agtcctgccg gtccagagcg ccgtctggat aatcgagcag ttcagtgtgc gtaatgccca
     1261 gcaccctgga cgcggcttca agttcggcgc ggcgaatacg ggccaactct tcatcgctca
     1321 cgccctctcc gcgaaagctg cccgccgagc cttctgttaa gcagacgacg ctggtttcta
     1381 tgccctggtc atgggccata agcaaagcgc caccgaatgc accgctttcg tcatcggggt
     1441 gcgcagtaat gcacatcagg cgataaggca aaatgtcagc tcctcaatgt ccagtttaac
     1501 gttgaaacga gtagttttta gcctggaccg tggtatcgtt gcacctatga agaagttcgc
     1561 cttatttctc acggtttttc ttttttgttc tctattgcat gcacagcagc cgaaggcctt
     1621 gctctatctc acgcgcagcg ccgatggcgt gcgctccttt tatgcccacg cagacaagat
     1681 tgatattctc gtgcccacct ggtacgacgt ggatggcgat ggccttgttt ccggcggccc
     1741 tgatccagcg gtacttgccg cggcaaagca gcaccacatt gaggtgatgc ccattgtggc
     1801 gagcagtggt tttcagcagg cccctttcca taagctgatt accactccgg cagcatggag
     1861 caggatggta gatcaattaa ttgcggcctg caggcagaac ggatactctg gcttccagtt
     1921 cgattttgag aacatcgcgt ggaccgaccg cgacacattg agtcaggcag tggccaaggc
     1981 agcagaggcg ctccatcgtg aagggctgaa gctctccatc gcgaccgttc ccaatgctcc
     2041 cggggccccg ggacagagcg cgttttccgc atggctctat gcaaactggc gtggtgctta
     2101 cgatctgagc gcgctggcgc agtccgtaga cctgatctgc ctgatgacct atgaccagca
     2161 cacgcgctgg acagctcctg gaccggttgg cggttggggc tggacaacgg aaaatcttga
     2221 ttacgcactg aaggttgttc ccaaagaaaa gctctcgctc ggcattgcgc tctatgggta
     2281 tcactggttc gcaggtcctc cgcagaacga caagcccaac cccagcgccg aatacattgg
     2341 cggacaggac gcacttgatc ttgccagagc atacggcgga aaaattgaat gggacccggt
     2401 ggaccgcacc gcgtggacct ggttttatcg cgatgacatg cgcgaatgga tcttcttcac
     2461 cgatgaacat acgttccgcg aacgctatga tctggtgaaa caacgcggac tacaggggtt
     2521 ttgctcatgg gtcctcggac aggaagatcc gtctatctgg aatgttctgc ccagccataa
     2581 ataaagcccc cacagtgaaa cagcgcatcc ataaaattat gccaacagca acacagtttg
     2641 aaacgcgaca gcttgggaat tctgacctct atctgacccc gatcggctat ggagcctggg
     2701 ccattggcgg aggaaactgg gaatttgcct ggggagcgca ggacgacaac gaatccattc
     2761 agacgattga gcgcgccatt gagagcggta tcaactggat tgataccgcc gccgtctatg
     2821 gcctcgggca ctcggaagaa gtcgtcgccc aggcgctgaa acgtgtaagc agaaagcctt
     2881 acatcttcac caaatgctcc atgcgctggg acgcagaccg caaaatctac cgcagcctca
     2941 aggcagcctc gcttcaggaa gagctggaca attcccttcg ccgccttcag gtcgaaacaa
     3001 ttgatctcta ccagatacat tggcccaacc cggaaggtga aattcaagag ggatgggaga
     3061 cgctaggaaa attcaaaaaa cagggcaagg tgcgctacat aggtgtttcc aacttcaatg
     3121 tggagcagat gaagcgcgta cagaagattg cgcccatcac ttcgctgcaa ccgccctatt
     3181 ctctcctgaa ccggaacatc gaagctgaaa ttctgccgtt ctgccagaaa aacaacatag
     3241 gcgtcatcaa ctattcgcca atggtctccg gccttctgac aggcaagatg acgcgggaac
     3301 gcatcgccag tctgcctgcc gacgactggc ggaaacgcag cccaaacttc aatgagccga
     3361 agctctcgcg caatctgaaa ctggttgagc ttttgcggga gatcggcaaa gaacacaacg
     3421 tcgaaccggg agtcatcgcc attgcatgga ccctgcggca tcccgcgatt acagcagcca
     3481 ttgttggggc gcgccgccct gaccagatag acggcatcct tcccgcagcc gccttccgcc
     3541 tgcgcgatga tgaaattgcg cggatcgaag acttttttca cgcaaaccca taaaccagaa
     3601 agcctgcggc tgtgaagtat cctgaagttt gggagaacag atgtctatct ttcatgaccg
     3661 ccgcgatgaa ctggaaaaat acgaatttat gatgggcacg acgcgtgggc ggctcgccgt
     3721 gtcgctcgac gcgctgaccg atgcgctcat tctcattggc cagcacggag tttactgcac
     3781 gtcgaaccgc aacccgtccc ggcctgcgct tgaccttgaa acggtcatgg gggagatcaa
     3841 cggtgccaaa gaactcatcc agtctgtaat gcaggacctg gagcagatca ggaatcagca
     3901 gtaatgaccg tcccgcttct ataatttaac tttcattgca cccctcaaaa gaaagtcgag
     3961 aagatcatgc cgtcgttaga aggaaagact gcgcttgtta ccggagcttc acagggaatt
     4021 ggacgcgcct gcgccatcgc actggcccgt gccggggcca gtgtggcact ggccgcgcgt
     4081 aacgaatcga agctgaacga agttgccgcg gagattaccg caaacggcgg ggcggcgaag
     4141 gcctttgttc tcaatgttgc aagcgaagag tccattaaga caacagccaa agctgttctc
     4201 gatcattttg gcactgtgca tattctcatc aacaacgccg gcatcacgaa ggacacgctc
     4261 ttgctgcgca tgaagcgttc cgattgggac gacgtcatca acaccaacct gaccggaaca
     4321 ttcctgctga cccaggcact catcggctcc atgatgaaag cgcgatgggg acgcatcatc
     4381 aacatctcca gcgttgtagg cgaaaccggt caggcagggc aggccaatta cgcagccagc
     4441 aaggccggcc tcatcggctt tacaaagtcg ctggcacgcg agctggcctc gcgcaacatt
     4501 acggcaaacg cagtcgcacc cggatacatt gagaccgcaa tgaccgctgt gctcgatgaa
     4561 aaacaacgcg tggccatgct ctcgcaggtg ccactgggcc gcgcaggtac agatgaagat
     4621 atagcgaatg ccgtccgctt ccttgcctcc gatgaggccg cctacatcac cgggcacgtc
     4681 ctggatgtaa acggcggcat gtttatgggg tgattcggga ggggtccctc cttttttttt
     4741 cgctcctttc gcctgctgag gaaaatttta gccgtcccga acgaccagcc gtggcaagat
     4801 tgcgtcaggc cggggtgact tgccattcca ttttctgtcc aatggggcgt agcttcttgt
     4861 acagaaatgg agacgtctca atgaatcgct ttgtccttgc tgcggctgtc ttcagtctgt
     4921 ccggactatc tcttgtcatg gccgctcaga cagctacgaa acccgccgta ctcgttgagc
     4981 ttttcacttc ggaaggctgc tcgagctgtc ctccggcgga caatctgctc gcagcacttg
     5041 accgtgaaaa ggggtaccac ggcattccgg tcgtcgttct cagcgagcac gtggactact
     5101 gggaccacga ggggtggcgt gacccgttct cttcgccaga ctggacacaa agacagaaca
     5161 attacaacga gcgctttcat ctgcaaagtg tctatacccc gcagatggtg attgacggaa
     5221 cgcagcaggt caacgggagc gacgggccag cgatcgcgca tgcgcttgaa aaagcggctg
     5281 tgcgcgcgca gcacgtttcc attgaaataa aggatgcagc gtggtctggc agtgagttgc
     5341 aggcgacggt gagtattcct gaaaccgatg caaagggcgc ggacatttat gctgtgctcg
     5401 ccgatgatca ggacatgtcg gatgtaaagc gaggtgaaaa cgccggacac accctcacca
     5461 acgttgctgt tgcccgtgtg ctgcacaaag tgggtaagct tcgggggcca tacaatggtc
     5521 agattcggat ttctctcccg agtggtatgg agcgcggaaa gatgcggctg atagtctttg
     5581 cgcaaaaagg
//
LOCUS       DASRBV010000305         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_274291, whole genome shotgun sequence.
ACCESSION   DASRBV010000305 DASRBV010000000
VERSION     DASRBV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274291"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="purD"
                     /locus_tag="VFA02_11300"
     CDS             complement(<1..965)
                     /gene="purD"
                     /locus_tag="VFA02_11300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZU10476.1"
                     /translation="MKVLVIGSGGREHALAWSIRKSPRVAEVVCAPGNGGIAQVARLA
                     PANQKDINDLLRVVATENPDLTVVGPEAPLALGLVDELQRRGLFVFGPTRAAAMLETS
                     KSFAKRFMQRHKIPTANYAVCSTQAEALASLDLFHLPVVVKADGLAAGKGVLICQTKK
                     EAQEAIAGLFSGKLLGTVETSVVIEEFLEGEELSFLVLSDGKHVAPLVPAQDHKRIGE
                     GDTGPNTGGMGVYSTDAMLDPQMRDWILHHIVQPTVDGMAAEDTPFAGVLYCGLMMTA
                     RGPMVLEYNARFGDPETQAILVRLESDLVDALEACAKGQLSGTELR"
     gene            1188..2510
                     /locus_tag="VFA02_11305"
     CDS             1188..2510
                     /locus_tag="VFA02_11305"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZU10477.1"
                     /translation="MAPLQAQSTATTRQKLPGLRSSELWYYFGPAFVASIAYIDPGNF
                     ATNFDGGARFGYRLLWVLLWSNAMAILIQYLAAKLGIATGRTLPQNCRDHFSRPATIG
                     LWVAAEISALATDLAEFLGAALGFYLLFGPWLAGFGWSKTEILLAAAIVSAVCVFAIL
                     ALELYGFRKLELAIMGFVCVIGICYAVEMFFIHPDWKAIAYHTLIPTIDSRSIYIAVG
                     MLGATVMPHVVYLHSALVQPRVREETTDTPRRRYLARLRHLRFETIDVFAAMNGAWLV
                     NSAMIVVSAAAFAGRHWQNGMSIEEAHRTLGPLLGHTSAVVFAIALLCSGLSSSTVGT
                     MAGQVILEGFLDVKFSIFLRRFLTLIPAIVVIATGLDPLKILILSQVCLSFTLPFALI
                     PLLLLTGNSEVMGEFVNHRTTRSLGWIAVTVILALNGFLLWQTFWGQG"
     gene            2738..>3966
                     /locus_tag="VFA02_11310"
     CDS             2738..>3966
                     /locus_tag="VFA02_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZU10478.1"
                     /translation="MSQVGNSFGSRSELKSGNRTYEIFRLDALEKKGINLSRLPYSLR
                     ILLENLLRQEDGKSVTADDIEFLARWDPKAEPSREIAYMPARVLMQDFTGVPAIVDLA
                     AMRDAMKELGGDPQKINPLQPAELVIDHSVQVDEFGTPRSYELNTMLEFQRNRERYAF
                     LKWGQSAFRNFSAVPPGMGICHQVNLEYLARVVFTTNGDKPRAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLVPQVVGFKLTGKLREGATATDLVLTVTEMLR
                     KLGVVGKFVEFYGPGVSELPLADRATIGNMAPEYGATCGIFPVDAETLRYLKLTGRSN
                     EQIQLVETYYKEQGLFHTAHSPEAEYSQTISLDLSTVEPSVAGPKRPQDRVRLSEAGT
                     NFRKQLPNLLGPNADKNG"
BASE COUNT          794 a         1219 c         1090 g          863 t
ORIGIN      
        1 cgcagttccg tgccgctcaa ctggcctttg gcgcaggctt cgagcgcgtc cacaaggtcg
       61 ctttccagcc gcaccagaat ggcctgcgtc tccgggtcgc caaagcgtgc gttgtattca
      121 agcaccatcg ggccacgggc cgtcatcatc agcccgcagt acagcacgcc cgcgaaggga
      181 gtgtcctcgg cggccattcc gtcaacggta ggctgcacaa tgtggtgcag gatccagtca
      241 cgcatctggg gatcgagcat tgcatcggtc gaatatacgc ccatgccgcc cgtgttcggg
      301 cctgtgtccc cttcgccgat gcgcttgtgg tcctgcgcag ggaccagtgg cgcaacgtgc
      361 tttccgtcgc tcagcaccag aaacgaaagc tcctcacctt cgaggaactc ctcaatcacc
      421 acgctggttt caaccgtgcc cagcaatttt ccgctgaaga gtccggcgat cgcctcctgc
      481 gcctcctttt ttgtctggca gatgaggacc cctttacccg ctgccaaccc atcggccttg
      541 acgacaaccg gaagatgaaa gaggtcgagc gaggccagcg cctctgcctg cgtagagcac
      601 acagcatagt tcgctgtcgg aatcttgtgc cgctgcataa aacgcttggc gaaggacttg
      661 ctcgtttcaa gcatcgcggc ggcgcgcgtc gggccaaaaa cgaacagtcc gcgacgctgc
      721 aattcatcta caagaccaag cgccagtggc gcctcggggc cgaccaccgt caggtctggg
      781 ttttccgtcg caaccacgcg cagcaggtcg ttgatgtctt tctggttggc cggcgccaga
      841 cgcgccacct gcgcgatgcc cccgtttccc ggcgcgcaca ccacctcagc gacccggggc
      901 gacttgcgaa tagaccaggc cagcgcgtgc tcgcggcctc ctgaaccaat gacaaggacc
      961 ttcatctttc tcctgtgttc tgttcaaagg ctatcgcatt ttcagagaca agtgtcaggt
     1021 gctccgcatc tcggagagat gtgagggttt gcagcggatt gcccatccag ggcttcctct
     1081 tcgtcttgca cgggaaaagc aggaaaataa gaattgttaa tgaattttag ttaactatgg
     1141 tttacttatt atggaaacca tcttatactc aggaacagaa aacggcgatg gcccctctcc
     1201 aggcacaatc caccgcaaca acacggcaaa aactgcctgg ccttcgttcg tctgaactct
     1261 ggtattactt cggacctgcc tttgtcgcca gcatcgctta tattgatccg ggaaatttcg
     1321 ccaccaactt cgatggcggg gcgcgcttcg gctaccgtct gctgtgggtg ctgctctggt
     1381 cgaatgccat ggcgatcctc atccagtacc tggccgcaaa actgggcatc gccaccggca
     1441 ggaccctccc gcagaactgc cgcgaccatt tctcccggcc tgcaacgatc ggcctctggg
     1501 tggcggcaga aatctccgcc ctggccactg acctggccga gtttctgggc gccgcgctcg
     1561 gcttttatct gctgtttggc ccgtggctgg caggttttgg ctggagcaaa acagaaatcc
     1621 tgctggcagc ggccattgtt tccgctgtat gtgtcttcgc cattctcgct ctggagctat
     1681 acggcttccg caagctggag ctggccatca tgggcttcgt ctgcgtgatt ggcatctgct
     1741 acgccgtgga gatgtttttc attcatcccg actggaaggc catcgcttat cacacgctca
     1801 ttccaaccat agacagcagg agcatctaca tcgccgtcgg aatgctgggt gccacagtga
     1861 tgccgcatgt agtctatctg cattccgcgc tggtgcaacc acgcgtccgc gaagagacca
     1921 ctgacacgcc tcgccgcaga tatctggcgc gtcttcggca tcttcgcttt gagacgatag
     1981 acgtcttcgc tgccatgaat ggcgcctggc tcgtaaactc agccatgatc gtggtctccg
     2041 ctgcggcatt tgccggacgc cactggcaga acggcatgtc tattgaagag gcccatcgca
     2101 cacttgggcc gctgctcggc catacctcgg ccgtggtctt tgcgattgcc ctgctgtgct
     2161 ccggtctgtc atcttccact gtaggcacaa tggccggaca ggtcattctg gaaggttttc
     2221 tcgacgttaa gttcagcatt tttctgcggc gtttcttaac actcattcca gccattgtgg
     2281 tcatcgccac aggactcgac ccactcaaaa ttctcatcct ctcgcaggta tgcctgagct
     2341 ttacgctgcc ctttgcgctc attccgctgc tcctgctgac cggaaactcc gaggtcatgg
     2401 gcgaatttgt caaccacagg accacccgca gcctcggctg gatcgccgtt accgtgatcc
     2461 tggccctgaa cggctttctg ttgtggcaga cattttgggg acagggataa atggaagcag
     2521 ggaggacatc cggccgcgtt aaaatagtcg cagagtcatg aacctcgtcc cgccatgcct
     2581 gaagcgtttt cgtcgcggcc gtccagaccg tggacgacag cttattcgct gactaatctt
     2641 gagtgttaga agttttcccg cggaaccggg cgcagccgtg cgtgctgccg ttcgcgtgga
     2701 ctggaattcc cttctcgcaa agaaagaaag gacacctatg agccaggttg gaaacagttt
     2761 tggcagtcgg tcagaattga aatccggcaa tcgtacttac gaaatcttcc gtctcgacgc
     2821 gcttgagaaa aaaggcatca acctatcgcg tctgccctat agcctgcgca ttctgcttga
     2881 gaacctgctg cgacaggaag acggcaagag cgtcactgct gatgatattg aatttctcgc
     2941 caggtgggac cccaaagccg agccttcgcg cgaaatcgcc tacatgcctg cacgcgtcct
     3001 catgcaggac ttcaccggag ttcccgccat cgtagatctt gcagccatgc gcgatgcgat
     3061 gaaggaactg ggtggcgacc cgcagaagat caatccgctc cagcctgcgg aactggtcat
     3121 tgaccactcg gtccaggtag acgagttcgg cacgcccagg tcatacgagc tgaacaccat
     3181 gctcgagttc cagcgcaacc gcgaacgcta tgcttttctc aagtgggggc agtcggcctt
     3241 ccgtaacttc tcagctgtgc ctcccggcat gggcatctgc caccaggtca atctggagta
     3301 cttggcgcgc gtggtcttta ccaccaatgg cgacaagccg cgcgcttatc ctgacactct
     3361 ggtcggcaca gactcgcaca cgaccatgat taacggcctt ggcgtcctcg gatggggtgt
     3421 aggcggtatt gaggccgaag cggccatgct cggtcaaccg gtttccatgc tggtgccaca
     3481 ggtcgtcggg ttcaagctga caggcaaact gcgcgagggc gcaacggcga ccgaccttgt
     3541 tctgaccgtg acagagatgc tgcgcaagct gggcgttgtc ggcaagtttg tcgagttcta
     3601 cgggccggga gtcagcgagc ttccgttggc tgaccgggcc accatcggca acatggcccc
     3661 tgaatacggc gcgacgtgcg gcatcttccc cgtggatgca gaaacactgc gctacctcaa
     3721 gctcacaggc cgcagcaatg agcagattca actcgtcgaa acctattaca aggaacaggg
     3781 cctcttccac accgcccact cacctgaagc agagtattct cagaccatct cgcttgatct
     3841 ttcaacagtc gagccgagcg tcgcaggacc gaagcgtccg caggaccgcg tgcgcctgag
     3901 cgaagctgga acgaacttca ggaagcagct gcctaacctg cttggaccaa atgccgacaa
     3961 gaacgg
//
LOCUS       DASRBV010000306         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_531968, whole genome shotgun sequence.
ACCESSION   DASRBV010000306 DASRBV010000000
VERSION     DASRBV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531968"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..2616
                     /locus_tag="VFA02_11315"
     CDS             199..2616
                     /locus_tag="VFA02_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10479.1"
                     /translation="MTILLQDIRFALRQLWKHPGFSLTAIFSLALGIAATVSVFSIIN
                     RVLLHPFPYKDADHIVEYNIRAKTEFEYTPAIYREQIAQLRQARSVEDVVLMDEQWQS
                     DTTMDIPQDVDMVFLSDNAFQFFGVPAMMGRTFLPSDDHQPVAVLTYQYWKRRFNGNP
                     SVVGQTMRVEGRAYTILGVMPRNFTWWDADVYIPLDTTDPSRHSYMTVIRLKPNISKA
                     QATTELMGIFQQMLHDHPHSMIAGMVPEVISIQQRFERSIGKTLYVLFAAVLLLLVIA
                     CVNVSILLLVRGSARTHEFAVRAAVGATTPRIMRQLLTEALILGITGAVLGVIITYQV
                     RPYLAALLPFELFPRGIDIPINFPVLLFSVAVAVLTSIFFGLFPALQLARPEIREIMQ
                     STTRKAAGSVSGKRLHSVLIAGQIALALVLLTAAATAIEGFRQLAKTDLGYDPARVAN
                     YSIPIHKGSYNTWEERASYFRQLRDRISQIPGVESASLALIGPPSSEWNFPAEILGRP
                     DTGSELVNVNFVSPEYFQQLRIPLIEGHIWEPAEAARGAHLVLVNKAFVKHYFPSGDA
                     LGHSVRVSKIVGRPPSDLAVDGSDGWAQIIGIVGDARNDGLDSPARPEIYYPFTYYMV
                     NIAQILVRSYGSPQALEQDVRRAIAQINPAQQVSEPVLTLEDRIKFQPEWARSRLSAI
                     LSTVFSILALLLASIGLYSVVSYSVSQRINEFGIRMALGAQRHHILRNVLSTAGVSIG
                     SGIVIGLVLSFGLHGLLAHWVEGATSNPLIMLAISLLLLVIALLACIVPAFRASMIQP
                     MKALRVE"
     gene            3016..5430
                     /locus_tag="VFA02_11320"
     CDS             3016..5430
                     /locus_tag="VFA02_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZU10480.1"
                     /translation="MVRLSRLLLGFSFLIAVTASGFAWEKGPESAEPPLLPCNAYGEA
                     RAYHGTVTDPSGATLPSATVVFTCGEERKSVVTTGDGSFSLPLQAGTYQVSIHASGFA
                     PLSRAITLTPNTEFAPLSFSLVLDTAVNTVTVSANSGFVPGDALSATKTNTPLIETPQ
                     SVSVITADDLTMRNPQSVNDAIAYTPGVAPAVYGQDTRFDWITIRGFAAQSYGMFRDG
                     LRWQSSDTSGHIEPFGLEQIDVLKGPASVLYGQSEPGGVISFVSKRPLAVPLMELEFD
                     HGSYDRHQGRADFGGPLDRQGKVLYRMVGLLRNSSTQVDFVPDNRRFMAPSLTWNLSG
                     QTALTFLTEWQYDRTNWEQFLPAQGSLLPNPNGVIPINRYLGEPSDFYRRQQWSAGYL
                     FEHHFNHVWTIRQNFRYANISNNGDVHYGFGFGTGTDYRTLQRYASGYLMSSHIYSTD
                     TQAEAVFDTGSVSHNVLVGEDYSNAYEQILSSSGNGGVIDVFHPVYGKTVTDYVPYSW
                     PHSPSSQNGIYLQDQMKVKQHLIFTLGGREDWAAFRTRDTLVPGSYQRQNDSKFTGRA
                     GVTYLTDAGLAPYFSYSTSFVPTTGTNLNGVAFKPTTGKQYEVGAKYQPRNWNGFLTA
                     SFYNVTEQNVQTTDPANPNNTVQTGEVRSRGVELEGLTSLAFGLKLHAGYSYDDMVVT
                     KANDASLGHRPVLVPEQTVGLLLDYTLQRTSLKGMELGGGARYTGITAGAPDGSLPVP
                     GYTLYDAELGYSVHGLRFAVNATNLADKHYVAVCSTIAYCNYGAARNVIGSARWDMSR
                     LWHKSE"
     gene            5504..>6021
                     /locus_tag="VFA02_11325"
     CDS             5504..>6021
                     /locus_tag="VFA02_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZU10481.1"
                     /translation="MSGTEALMEKISRPPRRSLLWRLAHCPRETFLRRTLFQIHLWAG
                     IAVGLLATVVGISGSAIVYKHELEKRFTPALYQTSGAPGLPADVLIAIAKALHPGWSL
                     EYISTEEAGSTATSDPWIFYLAPPGSKPFEEDALVYLDPGTGRLLGETHRSTALHPAG
                     FVDWVAELHYRLL"
BASE COUNT         1246 a         1759 c         1625 g         1391 t
ORIGIN      
        1 gggatttttc aaagcaggcc tgagtctgcc aaccttggta accaacacac aacctgtcct
       61 gattacaaaa gttgttattg tgtttcccgg gttgtatatt tgtcgtacaa accaataaaa
      121 tcgcggtttc cacgctactt actcgtataa actactcctt agtctgcggt ccaattcttc
      181 ttaaaaatca gaggcgccgt gacgatactc ctccaggaca tccggtttgc attgcgacaa
      241 ttgtggaagc atccggggtt ttctcttact gctatcttct ctctggcatt aggcattgca
      301 gcgactgtct cagttttcag tatcatcaat cgtgtcctgc tgcacccgtt tccatacaaa
      361 gacgcagacc acatcgtcga gtacaacatc cgcgccaaga cggagtttga atacacgccg
      421 gcgatttacc gcgaacagat cgcgcaattg cgccaggcac gctctgtcga agatgtcgtg
      481 ctgatggatg agcagtggca gtctgacacc accatggaca ttccgcagga cgtggacatg
      541 gtgtttcttt ccgacaatgc cttccagttc ttcggcgtcc ctgcgatgat gggccgcacc
      601 tttctgccgt cggacgacca tcagcctgtg gctgtactca cataccagta ctggaagcga
      661 cgctttaatg gaaatccgtc cgtcgtggga cagaccatgc gcgtggaagg tcgcgcctac
      721 accattcttg gcgtgatgcc gcgtaatttc acctggtggg atgccgacgt ctacattcct
      781 ctggacacga cagacccaag ccgtcattca tacatgaccg tcatacgcct gaagccgaac
      841 atcagcaagg cacaggccac tacggagctt atgggcatct tccagcagat gcttcatgat
      901 cacccgcatt ccatgattgc agggatggtc ccggaggtca tcagtattca gcagcggttt
      961 gagcgctcca tcggcaagac actctatgtt ctgtttgcgg ccgtcctgct gctgcttgtc
     1021 atcgcctgcg taaatgtttc catcctgctg ctggtgcgcg gctcggcccg tacacatgag
     1081 tttgccgtgc gcgccgcagt aggggcaacc acgccgcgaa tcatgcgcca gctccttaca
     1141 gaagccctga ttctgggcat taccggggcc gtgttgggtg tcatcattac gtatcaggtc
     1201 cgtccctatc tggccgcttt actgccgttt gagctttttc cgcgtggaat tgacattccc
     1261 atcaattttc cagtgctgct cttcagcgtg gccgtcgctg tcctcaccag tattttcttc
     1321 gggcttttcc cggcattgca gcttgcgcgg cctgaaattc gtgaaatcat gcaatccact
     1381 acacgcaagg cggcgggcag cgtttccgga aagcggctgc acagtgtcct gattgccggg
     1441 caaatcgccc tggcccttgt actgctgacg gccgccgcta ctgccatcga aggcttccgt
     1501 caactggcga agaccgacct gggctacgac cctgcgcgcg ttgccaacta cagcattccc
     1561 attcacaaag gctcttacaa cacatgggaa gagcgcgcca gctacttccg ccagttgcgc
     1621 gaccgtataa gtcagatacc gggtgtggag tcggcctcgc ttgcgctgat tgggccgccg
     1681 agcagtgaat ggaattttcc cgccgaaatc ctgggccgtc ctgacactgg ctcagaactg
     1741 gtgaatgtaa actttgtcag cccggaatat ttccagcagt tgcgcattcc gctgattgag
     1801 ggccatatct gggagccggc ggaagcagca aggggcgccc atcttgtcct cgtcaacaag
     1861 gcattcgtca agcactactt tcccagcgga gatgcgcttg gtcactccgt gcgcgtttcg
     1921 aaaatcgtgg gccgtcctcc ttcggacctt gccgtagacg gcagcgatgg ctgggcgcag
     1981 atcatcggca tcgttggaga cgcccgcaat gacgggctgg acagccctgc ccgtcctgaa
     2041 atctattacc cgttcaccta ctatatggtg aacatcgccc agatactcgt ccgctcgtat
     2101 ggaagcccgc aggcgctgga gcaggacgtc cgccgcgcca tcgcccaaat caatccggcg
     2161 cagcaggttt ccgagcctgt tttgacgctg gaagaccgga ttaaatttca gccggagtgg
     2221 gcgcgcagcc gcctgagcgc tattctttcc acagtctttt ctattttggc gctgctgctg
     2281 gccagcatag gactttacag cgtagtctcc tacagtgtct cccagcgcat caatgagttc
     2341 ggcatccgta tggccctggg cgcgcaacgg caccacatac tcagaaatgt tctgtccact
     2401 gcaggggtca gcatcggctc cggcattgtg attggactgg ttctgagttt cggcctgcac
     2461 ggcctgctgg cgcactgggt ggagggggcc accagcaatc cgctgatcat gcttgccatc
     2521 agcctgcttc tgctcgtgat tgcgttgctg gcctgcatcg tgcctgcttt ccgcgcctcc
     2581 atgatccagc ccatgaaggc cctgcgcgtg gaatagcttc caaagtgtcc aatgcccgtt
     2641 ggctggaaaa caccagcttt aatccgtggc tgattctggg agtttcctgt ctgttactcg
     2701 cagggctttg ctggcctgcc tcgccccggc gcgcaaggct gtggacgttg atccaatgac
     2761 tgctctgcgc tgcagcattt cactcctcca aaatccctat tcagggagaa ttgactcctt
     2821 ttcgctcgct ttgcttgaac taggactaaa tttgtcgtaa tttagctttg taattgaatt
     2881 caaagaggaa aacccccgcg gctgtctttt ggcgaagcct ccgcgagggc tttcctgaga
     2941 tcgccatgcc gttaggctga cgactggctc cgagtataga gccgcggctg ccacggcttc
     3001 aatacaggag cggaaatggt tcgtttgtcc cgtctgttgt tgggtttttc ttttcttatt
     3061 gcagtgacag catccggatt tgcctgggaa aaaggccctg aatctgctga acctccactt
     3121 ctgccatgca acgcttacgg agaagcgcgc gcttaccatg gcactgtaac agacccttct
     3181 ggggctactc ttccttcagc aaccgtggtc ttcacctgcg gagaggagcg aaagagcgtg
     3241 gtcaccacgg gcgatggcag cttttcactt ccgcttcagg ccggaacata tcaggtgagc
     3301 atccacgctt caggatttgc gcctctttca cgcgccatca cactcacgcc gaatacggag
     3361 tttgcgcctc tcagcttcag ccttgtgctg gacacggctg taaatacggt caccgtttca
     3421 gccaatagcg gctttgtgcc gggtgatgcc ctgagcgcca ccaaaacaaa cactccgctg
     3481 attgaaacgc cccagtctgt atctgtgatc acggccgatg acctgaccat gcgcaatccg
     3541 cagagtgtga atgatgcgat tgcctacact ccgggagtcg cgccggcagt ttacggacag
     3601 gacacgcgct ttgactggat cacgattcgc gggttcgccg cgcagagcta tgggatgttc
     3661 cgcgatggtc tgcgctggca gagcagcgac acatcagggc acatcgagcc gttcggcctt
     3721 gaacagattg acgtgctcaa aggtcctgct tctgttctgt atggacagag cgagccgggc
     3781 ggcgtcatca gctttgtctc gaagcgccct ctggctgttc ctttgatgga gctcgaattt
     3841 gaccacggca gttacgaccg tcatcagggg cgcgctgatt tcggaggccc gctcgaccgt
     3901 cagggcaagg tgctctaccg catggttgga ctgctcagaa acagcagcac gcaggtagat
     3961 tttgtcccgg acaaccggcg cttcatggcc ccctccctta catggaacct cagcggccag
     4021 accgcgctta catttctgac ggaatggcaa tacgaccgca ccaactggga gcagtttctg
     4081 ccggcccagg gaagcctgct gccgaatccc aacggagtga ttcctatcaa ccgctatctg
     4141 ggcgaaccgt ctgattttta ccggcgccag caatggagcg cagggtatct gtttgaacat
     4201 catttcaatc atgtctggac aattcgccag aacttccgtt acgccaacat ctccaataac
     4261 ggcgatgtgc attatggctt cggttttggc accggcaccg attaccgcac gttgcagcgc
     4321 tacgcttcgg gatacctgat gagctcgcac atctattcga cagacacgca ggccgaggct
     4381 gtattcgata caggtagtgt ttcgcacaat gtgctggtcg gcgaagacta ctcgaacgca
     4441 tatgagcaga tactctcctc ttccggcaac ggcggcgtga tagatgtatt tcatcccgtg
     4501 tacggaaaga cagtaacgga ctatgtgccc tattcctggc cgcactcgcc ctcctcgcag
     4561 aacggcattt atcttcagga ccagatgaag gtgaagcagc acctcatctt tactcttggg
     4621 ggacgcgagg actgggccgc cttccgcacg cgcgacactc tcgtccctgg tagctatcag
     4681 cgccagaacg acagcaaatt cacaggccgc gccggcgtga catatcttac agatgcggga
     4741 cttgcgcctt acttcagcta ctcgacctca tttgtgccca ccacaggcac aaatctaaac
     4801 ggagtggctt ttaagccaac taccggaaaa cagtatgagg ttggagcaaa atatcagccg
     4861 cgcaactgga acggtttcct gactgcatcg ttctacaacg tgactgagca gaatgtgcag
     4921 acaacagacc ctgcaaaccc taataacacc gtgcagaccg gggaagtgcg ctcacgcggc
     4981 gtagagctgg aaggcctgac cagtctcgca ttcggactga agctgcacgc tggatacagc
     5041 tacgacgaca tggtggtgac caaagcgaat gatgccagcc tggggcaccg tccggtcctt
     5101 gtgccggagc agacggtggg ccttctgctc gattacacct tgcagcgcac atcgctgaag
     5161 ggcatggaac tgggcggagg tgcgcgctat accggcatca ccgcaggcgc gcctgacggc
     5221 tctctgcccg tgcccggata cacgctctat gacgcggagc tgggctacag cgttcacgga
     5281 ttgcgctttg ccgtaaatgc caccaatctg gcagacaagc attatgtcgc agtgtgcagc
     5341 acgattgcct actgtaacta tggggctgcc cgcaatgtca tcggcagcgc gagatgggac
     5401 atgtcgcggc tctggcacaa gagtgagtag acacgctgtt agggtaccca tcttgcaggt
     5461 agcgaaggat gaaagagcaa agtgaaggtt gaggaaaatt agcttgtcag gtacagaagc
     5521 tctcatggaa aaaatcagca ggcctccccg gcgaagcctg ctctggcgcc tggcgcactg
     5581 tccacgcgag acgtttcttc gacgcacgct ttttcaaatc cacctctggg caggaattgc
     5641 cgttggcctg ctcgccacag ttgttggcat ttccgggagc gccatcgtgt ataagcacga
     5701 attggagaag cgtttcacgc cagcgttgta ccagacgtct ggtgccccag gactgcccgc
     5761 agatgtcctg atcgcgatcg ccaaagcgct gcatcctgga tggagcctcg aatacatctc
     5821 cacagaagaa gccggttcaa cggcaacttc cgacccgtgg atcttttatc ttgcccctcc
     5881 gggcagcaaa ccgtttgaag aggatgccct tgtatatctc gatccgggga caggcaggtt
     5941 gttaggagaa actcatcgct cgacggcact gcatccggcg ggctttgtag attgggtcgc
     6001 cgaactccac taccgccttc t
//
LOCUS       DASRBV010000307         6153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_532048, whole genome shotgun sequence.
ACCESSION   DASRBV010000307 DASRBV010000000
VERSION     DASRBV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532048"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFA02_11330"
     CDS             <1..381
                     /locus_tag="VFA02_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZU10482.1"
                     /translation="RTLKIHGYRKHVDDLGDLWVYGSDADAVLQIAARDPQLGQPLHP
                     ALPYIGAEAVWAARQEMARTVEDVLARRTRALFLNARAASEMAHPVAQIMARELHKEE
                     SWIVQQTAEFRALAQQYSLNGEEK"
     gene            378..1109
                     /locus_tag="VFA02_11335"
     CDS             378..1109
                     /locus_tag="VFA02_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZU10483.1"
                     /translation="MIRSPFFGEFMGTLVLILLGNGVVAGVLLKRSKAENSGWMVITT
                     GWAFAVVCGVFVAVLCGSADAHLNPAITLAMAVQTGSFNKCLPYISAQLLGAFAGAVL
                     VWLHYLPHWKLTEDVNAKRATFCTIPAVRHLAANLFSEIVGTAVLVLIVGAVSSKLVL
                     STGAAAGLSPYLVGCIVWSIGLSLGGTTGYAINPARDFGPRIAHSVLPIAGKGPSDWT
                     YAFVPILGPCIGGILAGWLLRGLGA"
     gene            complement(1142..1846)
                     /locus_tag="VFA02_11340"
     CDS             complement(1142..1846)
                     /locus_tag="VFA02_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10484.1"
                     /translation="MWQQVEQSLHDSMWRVFSKVAQLLPGILAFLVALLIFLLIAWGA
                     AVIVRRVLVAVDFDNRTKSTTNALTELAPQQTPTLLVTRVVFWAFVAIGIMVGVSAFE
                     AASAEYGISAYVFAYLPHVIGAAVLLFFGTVIGRFLSRSVLITAVNLNLQYARLLATG
                     VRWLIYVLTAAMVLDHLSIGGDIVPLAFGILFGGIVLALALAVGLGSRDLVSRSLERE
                     AVRPGEPPVEEKIHHF"
     gene            complement(1860..3071)
                     /locus_tag="VFA02_11345"
     CDS             complement(1860..3071)
                     /locus_tag="VFA02_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10485.1"
                     /translation="MKEEQIRDSGQTTAAETPGATVLLGITGTVDSAILQQRVQELGA
                     AFDARIIVAAPGLSETNESSIQFCSYALPASSPENLPWIITSMAQRAICSLAVELQAD
                     VCVLLHSDLLMLRADLVQWFAQPVLEKRCDLVLPVYASGKFEGLLNSGILSPLSRSLY
                     GQRIQYPLPLDFSCSAEMCAKLSTPHPSHDLYADEMLWPATVAAVAGKQVYQFHVDVS
                     HARQTEGIELRTALASLVGSAYIEMEKYAAFWQRNRGSRPTPVFGAPAKQAAEGDGVD
                     VKPLIDSFLLGARNLQEIWGPVLPPVTLFELKKLSRVPPEEFRMPDELWVRIIYDFAL
                     AHRLRTISRSHLLGALTPLYLGWVASYVQEATASSPFAAEQRLEQLARSYEENKPYLL
                     SRWRWPDRFNP"
     gene            complement(3078..>6153)
                     /gene="treS"
                     /locus_tag="VFA02_11350"
     CDS             complement(3078..>6153)
                     /gene="treS"
                     /locus_tag="VFA02_11350"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZU10486.1"
                     /translation="FPSPLRDDGYDIADYLTVHPNYGTLDDFKRFLREAHDRGMQVMI
                     ELVINHTSDQHPWFQRARHAPPGSPERDFYVWSDTDQKYKDARIIFTDTEKSNWTWDP
                     VAQAYYWHRFFSHQPDLNYDNPMVMEEVLKILRYWLDMGVDALRLDAIPYIVEREGTN
                     CENLPETHSVIKKIRAAMDEGYANRMILAEANQWPTDVRPYFGDGDECHMAFHFPLMP
                     RIYMALRQEDRLPITEIMAQTPEIPETCQWGLFLRNHDELTLEMVTDDERDYMYLAYS
                     ADPRMRINIGIRRRLAPLVDNNRRRIELLNSILFSFPGTPIIYYGDEIGMGDNIYLGD
                     RNGVRTPMQWTADRNAGFSRANPAQLYSPVIMDPVWGYQAINVEAQHTDPSSLLNWMR
                     NMIALRKLFPVFGRGTLKFLPSANRKVLSYVRQSDGQSVLCLANLSRFPQPVLLDLAE
                     FEGMIPVEMLGYVEFPPITKAPYSLTLGPYEFLWFELQSALPPQEDDELGPERSLIEI
                     GNENDWHAILTGMNLEKLRSTVLPEFIAKQRWFGGKSKQIRQVTVSDWVVFEEGRSVY
                     LVATVKYDSGEEESYFVPLTLLFGEAASQVRSTRENAIVAPVRAAASEGVLCDALVSE
                     SACQSLLSLLRKQITWKSMAGMVRGQASSVLPALIAENAPAPPARISSAEQSNSSILF
                     GTQMIMKCFRRHEPGPNPDTEISQFLTESSGFRAIAPFGGAIEYARDGERPSTLAMLQ
                     GFVANEGDGWKWTLDKLENYYKFCAGRHDENAVRDFISKNYFEAARKLGRVTAEMHRA
                     LASSADDPDFAPETMEQHEIDELCRDFAEHAAHTLETLKANLGRLPEDALELGGLVAG
                     RRRRILGCFHGLSSVQSPVIRTRIHGDYHLGQVLRTSKGFVVLDFEGEPARPLAERRK
                     KQPPLKDVAGMLRSFSYAAWSALARYFIQTHTENQQMEQWAKFWIHAVSDEFLRAYLA
                     EMRNSALLPEKDLDLILDAYLLDKALYELAYELNNRPDWVRIPLMGIVSLLS"
BASE COUNT         1426 a         1727 c         1646 g         1354 t
ORIGIN      
        1 agaacgctga aaatccacgg ataccggaag catgtggatg atctgggtga tctgtgggtc
       61 tatggatctg atgctgatgc tgtgctgcag attgcagcgc gcgatccgca gcttgggcaa
      121 ccgcttcatc cagcgctgcc ctacatcgga gcggaggccg tatgggccgc gcggcaggag
      181 atggcccgca cagtagaaga cgtgcttgca cgacgcacgc gcgcgctctt tctgaatgcc
      241 agggccgcaa gcgaaatggc gcaccctgtc gcgcaaatca tggcccggga actacacaaa
      301 gaagaaagct ggatcgtgca gcagacagca gaattccggg cgctcgccca acagtacagc
      361 ctcaacggtg aagaaaagtg atacggtctc cattttttgg tgaatttatg ggcacgctgg
      421 tgctcattct gctcggcaac ggagtggttg ccggggtatt gctgaaacgt tcaaaggccg
      481 aaaacagcgg gtggatggtc ataaccaccg gatgggcctt tgctgttgtc tgtggtgtgt
      541 ttgttgcagt attgtgcggc agcgcagatg cccatctgaa ccccgctatt acgttggcga
      601 tggccgtaca gacaggaagc ttcaacaaat gtcttcccta catcagcgcg cagttactgg
      661 gtgcgtttgc aggggccgtc ctcgtctggc tgcattatct tcctcactgg aaattgaccg
      721 aggacgtcaa tgcgaaacgc gcaaccttct gcaccattcc ggcagtacgg catctggcag
      781 caaatctttt ttcagaaatt gttggcactg ccgttctggt acttatcgta ggagcagtaa
      841 gttctaaact cgtactttcg acgggagcag cagcgggact ctcaccctat ttggttggct
      901 gcatcgtatg gtctatcggg ctgtctctgg ggggaacgac cggatatgct attaatcctg
      961 cgcgcgattt tgggcctcgc attgcgcatt ccgttctgcc cattgccgga aaaggcccga
     1021 gtgactggac ttatgctttt gtgcccatcc tgggaccatg cattggaggc attcttgcgg
     1081 gatggctgct gcgaggattg ggcgcgtgat tacccacatc tctgcgagat gtgggcgtga
     1141 tctaaaaatg atggattttc tcctctaccg gaggctcgcc tggccttacc gcttcacgct
     1201 ccagagaacg gctgaccaga tcgcgcgaac ctagtccaac agcgagggcg agcgccagca
     1261 cgattccacc gaaaaggatt ccaaaagcca gcggaacaat gtcgccgcca atcgagagat
     1321 ggtcaagcac catggcggcc gtcaggacat agataagcca acgcactcca gttgcaagca
     1381 gccgcgcata ctgcagattg aggttgacgg ctgttatcag aacgctgcgc gagagaaaac
     1441 gaccaatgac ggtcccaaaa aacagcagca cggctgcgcc aattacatgc ggcagatatg
     1501 caaaaacata ggcagaaatc ccatactctg ccgatgcagc ctcgaatgca gacactccaa
     1561 ccatgattcc gatggcgaca aacgcccaga agacaactcg cgttaccaac agagtcggcg
     1621 tctgttgcgg ggccagttct gtcagcgcgt ttgttgttga cttcgtgcgg ttgtcaaaat
     1681 ctaccgcaac cagaacacgc cgtacaatta cagccgcgcc ccatgcgata aggaggaaaa
     1741 tcaacaacgc caccagaaag gccaatatcc ccggcaacag ttgcgcgacc ttactaaaca
     1801 cgcgccacat cgaatcgtgc aatgactgtt caacttgctg ccacatttta ctttcctttc
     1861 tacggattga atctgtcagg ccatctccag cggctcagga gatacggctt attttcttcg
     1921 tatgagcggg cgagctgctc cagacgctgc tctgctgcga acggagacga ggctgttgct
     1981 tcctggacgt aagatgcaac ccagcccaga tacagcggtg taagcgcgcc caacagatgg
     2041 cttctgctga tagttcgcag acggtgcgca agcgcaaagt catagataat gcgcacccaa
     2101 agttcgtccg gcatcctgaa ttcctccgga ggtacccggg acaacttttt caattcaaac
     2161 agcgtaaccg gaggcagcac cggcccccag atttcctgca ggttgcgcgc gcccaacagg
     2221 aaggaatcta tcagcggctt cacatccacg ccatcgcctt cagcagcttg ttttgccggg
     2281 gccccgaaaa ccggcgttgg ccgcgatcct cgattccgtt gccagaaggc ggcgtatttt
     2341 tccatttcaa tgtaggctga gccaaccagg cttgccaatg ctgtcctcag ctcaatgccc
     2401 tctgtctggc gggcgtgact tacatccaca tggaattgat agacctgctt gcctgctact
     2461 gcagccactg tggccggcca cagcatttca tctgcgtaga gatcgtgcga aggatgtgga
     2521 gtgctgagct tcgcgcacat ctccgcagag caactaaaat ccagaggcag aggatactga
     2581 atgcgttgtc cataaagcga acgcgaaagc ggcgacagaa tgccgctgtt cagcaatcct
     2641 tcaaatttac ccgaagcgta tacaggaaga acgagatcgc agcgcttttc cagcacaggc
     2701 tgggcgaacc attgcacgag gtccgcgcgg agcatcaaca aatcactgtg cagaagaaca
     2761 catacatctg cttgcaattc aactgccagc gagcagatag cgcgttgcgc cattgaagta
     2821 atgatccatg gcagattttc agggctggaa gcaggaagcg cgtaactgca gaattgaatg
     2881 ctgctctcgt tcgtttcaga gagtccaggc gcagcgacta tgatgcgcgc gtcaaaagca
     2941 gcgccaagct cctggacgcg ctgttgcagt attgctgaat ccacagtgcc ggttatgccc
     3001 agcagcactg tagcgccagg tgtttccgct gcggtggttt ggccgctgtc ccgaatttgc
     3061 tcttctttca tgactgttta cgacaacaaa gatacgatgc ccatgagtgg aatacgcacc
     3121 cagtccggac ggttgttcag ctcgtaggcc agttcataaa gcgccttgtc gagaagatac
     3181 gcatccagta tcaggtccag gtctttctcg ggcagcaggg cggaattgcg catctctgcc
     3241 agatatgccc ttagaaattc atctgaaact gcgtgtatcc agaacttcgc ccactgttcc
     3301 atctgctgat tttccgtgtg ggtctgaata aaataccttg ccaatgccga ccacgcggca
     3361 tagctgaagg agcgcagcat tccggcaaca tctttcagcg gaggctgctt tttcctgcgc
     3421 tctgccagcg gacgcgcagg ctctccttca aagtcgagca ccacaaatcc cttgctggta
     3481 cgcaacacct gccctaagtg atagtcgccg tgaatgcgcg tcctgatcac cggcgattgc
     3541 accgaggaga ggccatggaa gcaaccaaga atccggcggc ggcgtcctgc aacgagccct
     3601 cccaattcga gggcatcttc cggaaggcgg cccagatttg ccttgagtgt ttcaagcgtg
     3661 tgcgcagcat gttcggcgaa gtccctgcac aattcgtcta tctcatgttg ctccatcgtt
     3721 tccggtgcaa aatcggggtc atcggctgaa gaggccagcg cgcggtgcat ttccgcagtc
     3781 actctaccaa gtttgcgcgc agcttcaaag tagttcttgc tgatgaaatc gcggacagca
     3841 ttttcatcgt gcctgcccgc acagaatttg taatagttct ccagcttatc gagtgtccac
     3901 ttccatccat cgccttcatt cgcaacaaag ccttgcagca tggccaaagt ggagggcctt
     3961 tctccgtcgc gggcatattc aatggccccg ccaaagggag caatcgcgcg aaagcccgag
     4021 ctttctgtca aaaactggga aatctccgtg tccggattcg gcccaggctc gtgcctgcgg
     4081 aagcacttca tgatcatctg tgtgccaaac aggatggatg aattgctctg ttctgcagag
     4141 ctgatgcgcg caggaggcgc aggcgcattc tctgcaatca gcgcaggcag cacactgctt
     4201 gcttgcccgc gaaccatgcc tgccatagat ttccacgtta tctgcttacg cagcaacgaa
     4261 agcagcgact gacatgcgct ttcacttact agcgcatcac acaaaacgcc ttcgctcgct
     4321 gcggcccgca ctggagcaac gatcgcattc tcccgggtgg aacggacctg tgaagctgcc
     4381 tcaccaaata acaacgtcaa cggaacaaag taggattctt cttctccgga gtcgtacttg
     4441 accgtggcca ccagatagac cgacctgcct tcttcaaaca cgacccaatc gctaactgtg
     4501 acctgtctta tttgttttga cttgccgccg aaccaccgct gctttgcaat aaattcagga
     4561 agcacggtag accgcagctt ttcgaggttc attcctgtca gaatggcgtg ccagtcgttc
     4621 tcatttccaa tttcgatgag agagcgttcc ggccccagtt catcgtcttc ctgggggggc
     4681 agcgccgatt gcagctcaaa ccacagaaat tcatagggac cgagcgtaag cgaatatggc
     4741 gccttggtaa tgggaggaaa ctcaacataa cccagcatct ccactggaat catgccttca
     4801 aactcagcca ggtcaagcag cacgggctgc ggaaaacgcg aaagattcgc cagacagagc
     4861 acgctttgtc catcgctctg acgcacatac gacagcactt tgcgatttgc cgaaggaagg
     4921 aatttcagcg tgccgcgccc gaagaccgga aacagcttcc gcagcgcaat catgttgcgc
     4981 atccagttca ggagtgagga agggtccgtg tgctgcgcct caacgtttat ggcctgatag
     5041 ccccagaccg ggtccatgat cacagggctg taaagctgcg caggattggc gcgggaaaag
     5101 ccagcattcc ggtctgctgt ccattgcatg ggagtgcgca cgccgtttcg gtcgcccaga
     5161 taaatgttgt cgcccatccc gatttcatca ccgtagtaaa taatgggcgt gccaggaaag
     5221 gaaaagagaa tactgttcag cagttcaatg cggcggcggt tgttgtctac cagcggcgcg
     5281 agcctgcgcc gaatgccgat attgatgcgc atcctaggat cggcgctgta ggccagatac
     5341 atataatcgc gctcatcatc ggtgaccatc tcaagcgtca gctcatcgtg attgcgcagg
     5401 aagagtcccc actggcaggt ctctggaatt tccggcgttt gcgccatgat ttctgtgatc
     5461 ggcaagcggt cttcctgccg caacgccatg tagatgcgcg gcatgagcgg aaaatgaaag
     5521 gccatatggc actcatcgcc atcgccaaaa tacgggcgca catccgtcgg ccactgattg
     5581 gcttccgcaa gaatcatacg gttggcgtag ccctcatcca tagctgcacg aatctttttt
     5641 atgaccgagt gggtttcagg caggttctcg caattcgtgc cttcgcgctc cacaatgtat
     5701 ggaattgcat cgagtcgaag tgcgtctacg cccatgtcca gccaatagcg cagaatcttc
     5761 agcacttcct ccatcaccat aggattgtca taattcaaat caggctggtg cgaaaaaaag
     5821 cggtgccagt aataggcctg cgcaaccggg tcccatgtcc agtttgattt ttccgtgtct
     5881 gtaaagatga tgcgcgcgtc cttatatttc tggtccgtgt cgctccatac ataaaaatcg
     5941 cgctcaggag aacccggcgg cgcatggcgc gcacgctgaa accacggatg ctggtcagac
     6001 gtgtggttga tgaccagttc gatcatcacc tgcattccac ggtcgtgcgc ttcccgcaga
     6061 aaacgcttga agtcatccag cgtcccgtag ttcggatgca cggtcagata atcggcaata
     6121 tcgtaaccgt cgtcccgcag tggcgacggg aaa
//
LOCUS       DASRBV010000308         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_629112, whole genome shotgun sequence.
ACCESSION   DASRBV010000308 DASRBV010000000
VERSION     DASRBV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629112"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFA02_11355"
     CDS             complement(<1..524)
                     /locus_tag="VFA02_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU10487.1"
                     /translation="MVCGAGAFGLNHLRIYRDLERSGLPVHLAAIIESNEALAATLQK
                     EWSVPVYLSVGSCLKDHASGKLDIHAASVCVPTSAHFSVASAFLNAGIDVLVEKPMAA
                     TLDEADHLLDIAKKQGRILQIGHLERFNPAVTAVQQFLNAPMFFEVHRLSVFSPRSLD
                     VDVVLDLMIHDLDIV"
     gene            complement(548..772)
                     /locus_tag="VFA02_11360"
     CDS             complement(548..772)
                     /locus_tag="VFA02_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10488.1"
                     /translation="MDQPSITVNGARRNEDIALDLLKFVAMTTGVGRASAPATGFVAA
                     SAPKPEDHVNQLLELYSRCVQAVEGKGASR"
     gene            complement(1085..1864)
                     /locus_tag="VFA02_11365"
     CDS             complement(1085..1864)
                     /locus_tag="VFA02_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10489.1"
                     /translation="MAAPAQSNESMLANDLAHQVITNEIKMQDQDHSHGMYQEETVKN
                     GVKEINYTVQTPQGELSRLIAKNGQLLNEQERQAEDQRIEKYLNDPSAQRKKQQSSQE
                     DARKTRQLFQMLPDALTFSYAERNGDTVKLNFEPNPSFHPPSREAKVFHELEGQMIVN
                     VKQKRLMEIHGHLKDEVHFGILGHLNKGGTFDVRQEEVAPGLWEITLLKVNMQGKALF
                     FKTISVQQNEMRSHYRRVPDNLTLQQAKDMLDKQAGKPLTP"
     gene            complement(1942..2079)
                     /locus_tag="VFA02_11370"
     CDS             complement(1942..2079)
                     /locus_tag="VFA02_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10490.1"
                     /translation="MFGSFITAVNFVETMTLNDKGLIQRHCVYWGWKGVSVLQQNKYR
                     K"
     gene            complement(2320..2601)
                     /locus_tag="VFA02_11375"
     CDS             complement(2320..2601)
                     /locus_tag="VFA02_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10491.1"
                     /translation="MSNAMCCDAQTKCRWWRSAGGPISSGALLVLLPKCPMCIAAYLA
                     LWMGAGTAMSLVIHLRPLLEIVFAISVILLLIRWLHFRSRKYVAGWPLP"
BASE COUNT          598 a          701 c          780 g          647 t
ORIGIN      
        1 acgatgtcga ggtcgtgaat catcaggtcg aggaccacgt ccacgtcgag ggagcgcggg
       61 ctgaagacac tgaggcggtg gacctcaaag aacatagggg cattcagaaa ctgctgcact
      121 gctgtgacgg cagggttgaa ccgctccaga tggccgattt gcaggatgcg tccttgcttt
      181 ttcgcaatat caagcagatg gtcggcttcg tcgagcgtgg cggccatggg tttttctacg
      241 agaacgtcaa ttcccgcatt taagaaagca gatgctacag aaaaatgcgc gctggtcgga
      301 acgcagaccg acgcggcatg aatgtcgagt ttgccgctcg catggtcttt caggcaggaa
      361 ccaacggaga ggtagacggg aacgctccac tccttctgaa gagttgcagc aagggcttca
      421 ttggattcga taatggccgc gagatggaca ggaaggccgg agcgttccag atcgcgatag
      481 atgcgcaggt ggttgaggcc gaaggcgcct gccccgcaaa ccaccacgtt ttgtacagat
      541 gcagttgcta tcgcgatgct ccctttcctt cgacagcctg cacgcagcgc gaatagagtt
      601 cgagcaactg gttgacgtgg tcttctggtt tgggcgcgga ggcggcgaca aagcctgttg
      661 cgggtgcgga agcacgtccg accccggtgg tcatggcgac aaatttgagc agatcaagcg
      721 cgatatcttc attgcggcgt gcgccgttga ctgtaatgga tggttggtcc ataaaaacaa
      781 gtccctccga agcgatgcta tcaaatatgc gctgagcgcg tagtggaaag ccggaatgga
      841 tctacgccct cagggctgag aaacaagatg tgtggttcgt ctgtggcccg cccccgagga
      901 accggagacg gtttctacga atgacaaagg aagttcaccc acgcagtttg ccgatgggag
      961 agtatgaaca tagtgggtaa aaccatgtaa tgtcagaaag cccctactca cagcggtggg
     1021 agctggtcat ctgaaccagt cctccgcaac tcagaaaacg ggttctctga atccgccttt
     1081 tgattcatgg cgtgagcggt ttaccggcct gcttgtcgag catatctttg gcctgttgca
     1141 aagtcagatt gtcaggaacg cggcggtagt ggctgcgcat ctcgttctgc tgcacagaga
     1201 tggttttgaa gaacaaagcc ttgccctgca tgttgacttt gagcagggtg atctcccaaa
     1261 gtccgggggc cacttcttcc tggcggacat caaacgtgcc gcctttgttc aggtgtccca
     1321 gaatgccgaa gtgcacttca tctttcaggt ggccgtgtat ctccatgagc cgcttctgct
     1381 tcacatttac gatcatctgg ccttccagtt catggaacac tttggcttcg cgggaaggag
     1441 gatggaatga cggatttggc tcgaagttca gcttcacggt gtcgccgttg cgctctgcat
     1501 agctgaaggt cagtgcatcg ggcagcatct ggaagagttg tctggtcttg cgcgcgtcct
     1561 cttgcgagga ctgctgcttt tttctttgcg ctgagggatc gttcaggtat ttctcaatcc
     1621 tctggtcttc ggcctggcgc tcctgctcat tgagcagctg gccatttttc gcaatcaggc
     1681 gagaaagctc accttgcgga gtctggaccg tgtagttaat ttctttgact ccatttttga
     1741 ctgtttcctc ctgatacatc ccgtggctgt ggtcctggtc ttgcattttg atttcattgg
     1801 tgatgacctg gtgtgccaga tcgtttgcta acatgctttc attcgattga gccggggcag
     1861 ccatgccgca catgaggatg caaggcaaaa ggaccagcag ggaccatttg ttcatacgtt
     1921 tggtttgatg ctgttccccc ttcacttgcg atacttgttc tgttgcagga cgctgactcc
     1981 tttccatccc cagtaaacgc agtgccgctg aatcaggccc ttatcgttga gtgtcatggt
     2041 ttcaacaaag ttcaccgcag tgatgaatga tccaaacatc gcgcagccat ttcttggagc
     2101 caagggacca taaagcaaat gagatagaga aaatgcatca ggcatggtgt aaatcgccga
     2161 tactgcaaat tgcgctctgg accgagccgc atacgaaccc gcagctcgcg gcgaatgagt
     2221 ggtgagcggc gcttgagaag atcatgaaag ggcagttcga ttcttaccgc attcaatcag
     2281 ggccgatggg actcatgaaa aggccctgag ttgccgttct tacggtaacg gccaacctgc
     2341 cacatacttc cgggaccgga aatgcagcca tcggataagc agcagaatca ccgaaattgc
     2401 aaacacaatc tccagaagcg ggcgcagatg gatgaccaga gacatggccg tgcctgcgcc
     2461 catccagagg gccaggtatg ctgcaataca cattgggcac tttggcagca gcaccagcaa
     2521 tgcgcccgag cttatcgggc cgccggcgct gcgccaccat cggcactttg tttgtgcatc
     2581 gcagcacatt gcattactca tgggtgcagc atgcggctgc gggttttgcc gtttcgtaac
     2641 gatcgcggtg tcttacccac gccatgggcc acggcaggcc ctcttcatta cggccctttg
     2701 gtgacatatc gaggattgca tagata
//
LOCUS       DASRBV010000309         9771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_374935, whole genome shotgun sequence.
ACCESSION   DASRBV010000309 DASRBV010000000
VERSION     DASRBV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374935"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFA02_11380"
     CDS             <1..982
                     /locus_tag="VFA02_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10492.1"
                     /translation="LRGFVSISVPEKLAAMDWVHQPQQFSEALTAHLWATHQMDAFRA
                     AADEYAAAWREAKPEPEPAVPRLSIILIGQGCSSSPHPLFSKLRPYGTYFSHVDPANG
                     WDAIATTVTVRATKHQEKYQHWYIDGGEAADLPSRGVSVVSYAGLAPARSAILKRMQT
                     VINSGNGGPEALRTLLAQTHPEDIGLDVRNDATLAHFKTSILTEGSGTQIFSTTFAQW
                     TAREALRRAQPVTMLVRFAPRQRQLPMNELLSGQHLVNETDPVGSLIDADMGAYYTWI
                     NQQRLSGAEKSNFLAWFEGKNEAIAIGPSVPRGVISPSQMTIEKLIAMLG"
     gene            complement(1271..1993)
                     /locus_tag="VFA02_11385"
     CDS             complement(1271..1993)
                     /locus_tag="VFA02_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HZU10493.1"
                     /translation="MLSAGKAIKVSIYLSEGSTHHGASAYSSILDYLFFHRISGATVL
                     KGVAGFGADHHLHSAGTVEISEKLPVKIEFIESEEKVGELLPRLREMAGSGMIELQET
                     TVIKPAAPSRPQKSPAPQHVKIEGKAKLMQIFIGESDRWQGKPLHEALVQAMRAYDLA
                     GATVYRGILGYGAHRRVHKEKPFHLSHDASIMITAVDTEEKLKSFLPVVDQMVEEGLV
                     VLSDADIIKYAHRVEGESEHLP"
     gene            complement(2023..2400)
                     /gene="crcB"
                     /locus_tag="VFA02_11390"
     CDS             complement(2023..2400)
                     /gene="crcB"
                     /locus_tag="VFA02_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZU10494.1"
                     /translation="MQKYLLIAMGGALGSLARYWVGAAIVDRFGSKFPYGTFVINITA
                     CFIIGFSLTFLGERADLSPAWRFLIPIGFVGAYSTFSTFEWEIFINLQAGAFLIAALY
                     VGLSCLLGLAGVWCGVLVAKAVS"
     regulatory      complement(2505..2585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2858..6274
                     /locus_tag="VFA02_11395"
     CDS             2858..6274
                     /locus_tag="VFA02_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10495.1"
                     /translation="MNRRQLGFLTSIFTFFLFAWMAQAQENAVITGIVTDPSGAVVPQ
                     VTVTLTHMETGETRTTVTNNAGLYTFPSLHIGHYILKAEASGFKTFTRTDIVLNVAQT
                     LQENIPLALGTAGQTVTVQANALQVQSQTSEVSTLITGSQVTQLATNGRNVTALATLG
                     MGVSNTMSDYSGVNALTSNPSISFNGTRPSHNVFMIDNGEIYDRGCGGCFSVLPSIDA
                     LAEFQTLDSNYSPDYGIGSGGTITMVLKSGTHDFHGGLWEFNRNEAYDANSYFLNRNG
                     QSRPEHRLNLFGGNIGGPLWIPHVYNSDRKRTFFFVNEEWRRQIWGSAPTVTPTVPSS
                     DFPAAGQSLVYVPPASSTIIPVVPRTSDPAKLTLYAQDGLTPGQPFPNNTIPANLIDP
                     NAVLFMNTGAIPKPNVAGVNNYISSIPQTTNVREDLVRIDHTINSKLQLMGHYIHDAM
                     DQTLYPPLWSGGSYPTVGSVMQNPAWTAVIKLTQTLSSNMLNETAFNYDGNKIRLNPT
                     GIYKQPTGWSAQSFFPAANNLLSRLPEIDFGAPYNTNWRPSYFPWKNAAEDYQVRDDF
                     SWTKGVHSLKFGVSYMRFIKNQQLQANTNGVYNFNSTAFSGDAYVDFLLGFATSFNQL
                     EYMSTPHWVNNTYSLYVNDNWHVNQRLTLNLGFRYDALPHAYERYNRFANFVPADYDP
                     AQAPKFNADGSLDPSGPGFSVPPGFNSSFYTNGLKLAGVDGFPRGNVRNYWGTIMPRI
                     GFAADVLGDGKTVVRGGFGIFYERIQGNDVYNSALNPPFAFQPAANNVYFSNPHQSAI
                     TGATASTPYFPASLTNLKYDYAIPGTAQFSLGVQHELAPSTVAVVQYVGSTAWHQSDD
                     RNINTLPLNDLTDRQAVATKGANANLYRQFRGFAGITQEETTTNASYHSLQAGIRIEN
                     RHGLTLQLAYTYSHEIDIGSNDLNGISNPFNIEYDRGSGALDRRHIFNANYIYSFPFF
                     AHSQSALARSFLGGWQFSGITVAQTGVPSNVTYSTDVLGLGGGTTNRPNVSGKMRYPR
                     QQAAWFDKNLFTVPAPEWAGGNNHGFGNARKDTVVGPGRLNFNLSLFKAIPVTSNEAG
                     PRFELRFESFNTFNHTQFSGIDTGFTSGTFGQVTSTADPRVLQLGAKFLF"
     gene            complement(6352..7059)
                     /locus_tag="VFA02_11400"
     CDS             complement(6352..7059)
                     /locus_tag="VFA02_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HZU10496.1"
                     /translation="MTTVSNPSAPLTVKPQEVEKRERIVKRIARELRDGYYVNLGIGM
                     PTLVANHVPPGIEVVLQSENGILGVGPYPIAGQEDPDLINAGKETVTELPGTSFFSSA
                     DSFAMIRGGHIDLSILGAMEVDEVGNLANWMIPGKMVKGMGGAMDLVASARRVIVAME
                     HTTKDGRPKILKRCTLPITGLKVVHTIATEMAWIQVTPEGLVLEEIAPGLSVADVQAA
                     TEAKLHVSPNLKEMLLT"
     gene            7151..7372
                     /locus_tag="VFA02_11405"
     CDS             7151..7372
                     /locus_tag="VFA02_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZU10497.1"
                     /translation="MSFLLSGFPNTRPSIFLLVPTFLALYGTYDTVRCLRLRWSLYHG
                     RVLVLLYMDLMALAMIFFLLLYPYEGWLQ"
     gene            complement(7410..9161)
                     /locus_tag="VFA02_11410"
     CDS             complement(7410..9161)
                     /locus_tag="VFA02_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU10498.1"
                     /translation="MRLIAALCLLSPLAIGIFAQTSASTQPAPVYKTTVRQVVLDVVV
                     TGAKGEALPGLKKEDFSLLEDGKPQTISYFQEHTGAAPAQGTALPAMPPNVYTSFPLT
                     PETDSVNVLLLDALNTPMPDQAYVRQQMLKYLKQLPPGTRIAVFTLASRLRMVQGFTS
                     ESSQLLAALDGKKHKLLPQSSPLLDTDHTQEDFIEQLQNDPAMSNSLALSAMQQFNAD
                     TQTFRTDMRVRITLDALSELGRYLSSIPGRKNLLWFSGSFPFIINPDLDLPNPFQAMS
                     VYGDQIRETTDMLRLGQIAVYPIDARGLMVSPVYDASRRGPSNAAAFAKADMKFFQQT
                     AAEHQTMNTIAEETGGEAIYNTNGIQDAIRRVINNGSHYYTLAYTPTSPKLDGGYRHI
                     QVLLARSGYNLQYRHGYFAEDPEAAHKHTTTAPSDPFLPLMAHGLPSFSQIIYKVMMA
                     PENPQPSAMGPVVGDNKTLKGGLVRYQMDYAVAEPTLSFSTTPDGLHHGTVRFAAVAF
                     DQNGNVLNSTVQEVQLHLKPGTYSAFSQVGLQFRQELDLPKNAVFLRTGVYDPLSGRI
                     GTLEVPVQVTVLSSADK"
     gene            complement(9280..>9771)
                     /locus_tag="VFA02_11415"
     CDS             complement(9280..>9771)
                     /locus_tag="VFA02_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZU10499.1"
                     /translation="HKLSDYRGKFVVLEWTNKDCPFTRKHYESGNMQALQKEWTARGV
                     VWLTILSSAPGQQGYMTASEENAYMTKMKASPTAAILDPSGVIGHEYEAKTTPHMFVI
                     DPSGNVVYEGAIDDHPTTDPADIKVAKNYVSEALTEAMKGQRVAVSYTRPYGCSVKYA
                     GGE"
BASE COUNT         2253 a         2598 c         2614 g         2306 t
ORIGIN      
        1 tttgcgtgga tttgtatcca tatcagttcc agaaaagctg gccgcaatgg attgggtgca
       61 tcagccgcag cagttcagtg aagcgctgac tgcccacctg tgggccacgc accagatgga
      121 tgccttccgc gccgcggccg atgaatacgc tgctgcatgg cgtgaggcca agcctgagcc
      181 tgagccggcg gttccgcgac tgagcattat actcatcggt cagggatgca gcagcagccc
      241 gcacccgctc tttagcaaac tgcggcctta cggaacttat ttttcgcatg tggatccggc
      301 gaatggctgg gatgccattg caacgacagt tacagtgcgg gccacgaagc atcaggagaa
      361 ataccagcac tggtacattg acgggggcga ggcggcggac ttgccatcac gaggagtctc
      421 cgtggtctct tatgcggggc tcgcgcctgc tcgcagtgcc attcttaaac gaatgcagac
      481 cgtcattaac agtggcaacg gcggccccga ggcgctgcga acattgctgg cacagacgca
      541 cccggaggac atcgggctgg acgtgaggaa cgacgccacg ctggcccact ttaagaccag
      601 tattctgacc gaaggctccg gcacgcagat cttcagcact acttttgcgc aatggacggc
      661 ccgcgaggct ctgcgccgcg cccagccggt caccatgctg gtgcggttcg ctccgcgcca
      721 gaggcagctt cctatgaatg agttgctttc cggtcagcat ctagtaaatg aaacggatcc
      781 tgttggttca ctgatagatg ctgatatggg tgcttattac acatggatca accagcaacg
      841 cctttccggc gcggaaaaaa gcaactttct ggcatggttt gaaggtaaaa atgaagccat
      901 agcgattggg ccatccgtgc cgcgcggagt aatttctcca tcgcaaatga cgatagaaaa
      961 actgattgct atgctgggtt aaatgcgtaa ctctgcggcc atcctgggtg cttccacaaa
     1021 ctatacattg gccgtccagc gtcagccgcc tggcgcagtg cgatggtgga gctatccttt
     1081 tcattgggca gcatatcgca tctatgtttt gaagaaggat gcttctgtga aataacaaag
     1141 gcgatgggca ccattcactg tcaaaaagca tttttttcag atgcagggaa ataaattcaa
     1201 ttcagcgtga gagaagtcat ggcccctccc ggctttggca gaaatagcaa tttcatctaa
     1261 gcagacgggt ttatggcaga tgttcacttt cgccctcgac acggtgcgca tacttgatga
     1321 tgtccgcgtc agagagcacg acgagtcctt cctcgaccat ttggtctacg accggcagga
     1381 acgatttcag cttctcttcg gtatctacgg ctgtaatcat gatggaagca tcgtgtgaga
     1441 ggtgaaaagg cttttcttta tgcacgcggc gatgtgcacc gtatcccaga atgccgcggt
     1501 agactgtcgc tccggccaga tcgtatgcgc gcatcgcctg taccagcgct tcgtgcagag
     1561 gcttgccctg ccagcggtcg ctttcgccga tgaatatctg cattaatttt gctttgccct
     1621 caatttttac gtgttgcggg gcaggggact tttgcggcct tgatggcgct gctggcttga
     1681 tgacggtggt ttcctgcagc tctatcatcc cgctgcctgc catttcacgt agacgcggaa
     1741 gaagttcgcc gaccttttct tccgattcaa taaattcaat cttgaccggg agcttttcgg
     1801 aaatctcaac ggttcccgcc gagtgcagat gatggtctgc tccgaatccg gcaacacctt
     1861 tcaagacggt cgcgccggaa atgcggtgga agaaaaggta atcaaggata ctggagtaag
     1921 cagaggcccc atgatgggtt gagccttcac tcagataaat ggaaaccttt atggccttcc
     1981 cggcagacag catatcttct cccacacgaa ccatcattgc cctcacgaaa cagcttttgc
     2041 aaccagaacg ccgcaccata cgcccgccag gcccaaaagg caacttagcc caacgtagag
     2101 ggcggcgata aggaacgcac cggcttgcag gttgataaat atctcccact caaatgttga
     2161 aaaggtgctg taggcgccta caaagcctat gggtataaga aatctccatg ctggcgacag
     2221 gtcggcgcgt tcgccaagaa aggtaagaga gaacccgata ataaagcagg cggtgatgtt
     2281 gatgacgaag gtcccatacg gaaatttaga gccaaacctg tccacgatgg ccgctccaac
     2341 ccaatagcgg gccagcgaac caagcgcacc gcccattgcg atcaagagat atttctgcaa
     2401 ttcctgacgt tctcctttgg cacagaacca tccatctggc ccgtaaagtt ttcagcattt
     2461 ttttagggga aaccacgccg atggacaaag ctccacgaca gggtcccctg tcaggagtca
     2521 tcatctgtcc agccggtatt cggaagggac agcggttaag ggtgaactcc ttcaccacgc
     2581 tgagtctatg ggcaatatct taactcaaaa aacaggcatt tggatgtcac acatcgcggg
     2641 cagattttcc ggacgcctgt catttgacaa agcgttttac cgattgttaa tgtagctgcc
     2701 caggaaacat tttcatacag gaaacttggt ggattgtttc tgcaaaacaa cgagtaagga
     2761 atcagcccag caagtcccaa aagcactgca ttcagagtaa agaaaacatt ttcctcaatt
     2821 gtctgccgcg gctacagctc acttgcagga ggtcggtatg aacaggaggc agttaggatt
     2881 tctcaccagc atcttcacgt tttttctctt cgcctggatg gcgcaggcgc aggaaaacgc
     2941 agtcatcact ggtattgtca cagacccatc aggcgcagtg gttccgcagg taacagtcac
     3001 ccttactcac atggaaaccg gagaaacccg cactacggtc acaaacaatg ctgggctcta
     3061 cacatttccc agccttcaca ttggccatta catcctcaag gcagaggcaa gcggctttaa
     3121 gaccttcacc aggaccgaca tcgtgctgaa cgtggcccag acattgcagg agaacatccc
     3181 attggccctg ggcactgcgg ggcaaacggt caccgtgcag gcgaatgcct tgcaggtaca
     3241 gtcgcagacg agtgaagtta gtacgctgat tacaggaagc caggtcacgc agcttgccac
     3301 aaacggacgc aacgtcaccg cgctggccac gcttggcatg ggcgtctcga ataccatgtc
     3361 tgactatagc ggcgtgaacg cccttacctc gaacccttcc atcagcttta atggcacgcg
     3421 ccccagccat aacgtcttca tgattgacaa tggcgaaatc tatgaccgcg gctgcggcgg
     3481 atgcttcagc gtcctgcctt cgattgacgc actggccgaa ttccagacgc tggacagcaa
     3541 ttacagtccg gactatggca tcgggtccgg cggcactatc acgatggtgc tcaagtcagg
     3601 cacgcacgat tttcacggcg gactgtggga gttcaaccgc aatgaagcgt atgacgcgaa
     3661 cagctatttc ctgaaccgca atgggcaatc gcgaccagag caccgcctga atctatttgg
     3721 cggcaatatt ggtggaccgc tatggattcc gcacgtgtat aacagtgacc gtaagcgcac
     3781 cttctttttc gtcaatgagg agtggcgcag gcagatatgg ggaagtgctc ctacggtcac
     3841 gcccactgtt ccttcatccg actttccggc agcgggccaa tcgctggtct atgtgcctcc
     3901 cgccagcagt acgatcattc ccgtggttcc gaggacaagc gatccagcga agctcacact
     3961 ctacgcccag gatggtttga cgcccgggca gccttttccg aacaacacga ttcctgcaaa
     4021 cctgattgat cccaatgctg tgttgtttat gaatacaggg gcgattccga agccgaatgt
     4081 agctggcgtg aataactaca tttcctccat tccgcaaacg acgaatgttc gcgaagacct
     4141 ggtacgcata gatcatacga tcaacagcaa attgcagttg atggggcact atatccacga
     4201 tgcaatggac cagacactct atcctccgct gtggagcggc ggttcttatc cgacggtagg
     4261 cagcgtgatg cagaaccccg catggacagc ggtcatcaag ctgacgcaga cactttcctc
     4321 caacatgctg aatgagactg cgtttaatta tgacggaaac aagatccgcc tgaatcctac
     4381 gggcatttac aagcagccca cgggatggag cgcgcagagc ttctttcccg cagcaaacaa
     4441 tttgctcagc aggcttcctg aaatagattt tggcgcgccc tataacacga actggagacc
     4501 cagctatttc ccctggaaga atgccgcgga agattatcag gtgcgcgatg atttttcctg
     4561 gacgaaggga gtgcatagcc tgaagttcgg cgtgagctat atgcgcttta tcaagaacca
     4621 gcagcttcag gcgaacacaa acggcgttta taacttcaac agcacggctt tctcaggcga
     4681 tgcttatgta gattttctgc tgggctttgc cacgagcttt aaccaattgg agtatatgag
     4741 caccccacat tgggtaaaca atacgtattc tctctacgtg aacgacaact ggcatgtgaa
     4801 ccaacgtctg actctcaatc tgggcttccg ttacgacgcg ctgccgcatg cctatgagcg
     4861 ctataaccgc tttgccaatt ttgtgccggc tgattacgat ccggcccagg cgccgaaatt
     4921 caatgcagac gggagtctgg atccgagtgg ccctgggttt agtgtgcctc ctggatttaa
     4981 tagttcgttc tatacgaatg gactcaaact ggcgggcgta gatggctttc cgagagggaa
     5041 cgtaaggaac tattggggca ccatcatgcc gcgtatagga ttcgccgcgg atgtcttagg
     5101 agatggtaag accgtagtcc gtggagggtt tggaatcttc tatgagcgca ttcagggcaa
     5161 tgatgtttat aactcagcgc tgaatcctcc ttttgcattc caacccgcgg caaataatgt
     5221 ttatttctcc aacccacacc agagcgcaat tacgggcgca acggcatcta cgccgtattt
     5281 tcccgcaagc cttacaaact taaagtatga ttatgcaatt ccgggaacag cgcagttcag
     5341 tctgggagtg cagcatgagt tggcgccatc cactgtcgca gtcgtccagt atgttggctc
     5401 tacggcatgg caccagagcg atgatcgcaa catcaatacg ctgccgctga atgatctgac
     5461 cgaccgtcag gccgttgcta ccaaaggcgc aaatgcaaac ctgtaccggc agttccgtgg
     5521 ttttgccgga attactcagg aagagactac caccaacgcc agctatcact cgttacaggc
     5581 tggaatccgc atcgagaacc gccacggact gacgctgcaa ctggcctata cgtattcgca
     5641 cgaaattgat atcggcagca acgacctgaa cggcatttcc aatccgttca acattgaata
     5701 tgaccgcggc tctggcgcgc tcgaccggcg gcacatcttc aacgctaact acatctatag
     5761 cttcccgttt ttcgcacatt cgcagagtgc gctggcgcgc agttttctgg gaggatggca
     5821 attctccggc atcacagtcg cgcagaccgg agtaccttcg aatgtgacct actcaacgga
     5881 cgtgctgggg ctgggcggcg gcacgaccaa ccggcccaat gtctccggta agatgagata
     5941 tcccaggcag caagccgcat ggtttgataa aaacttgttt accgtgcctg ctccggagtg
     6001 ggcaggagga aacaatcacg gctttggaaa tgcgcgcaag gacacggtgg ttggccctgg
     6061 ccgcctgaac ttcaacctct ccttgttcaa agccatccca gtcacctcga atgaagccgg
     6121 cccgcggttc gaactgcgct tcgagtcctt taacaccttt aaccatacgc agttcagcgg
     6181 catagatacc ggttttacca gcgggacctt tggtcaggtc acctctacgg ccgatccacg
     6241 tgtcctacag cttggagcaa agttcctgtt ctgacacttt ggtgagggac gggtgtctca
     6301 tcttctcatt tcaagatgaa gagtgggaca cccgcgagga ttaagaggcc cctatgtcag
     6361 gagcatttct ttcagattcg ggctgacatg tagttttgct tccgtagccg cctgcacgtc
     6421 ggccacgctg agtcctggag caatctcttc cagtaccaga ccttcaggcg tgacctgtat
     6481 ccatgccatc tccgtagcaa tggtgtgcac aaccttcagg ccggtaatgg gtagtgtgca
     6541 gcgcttcagg attttcggac ggccgtcttt tgtggtgtgc tccatggcta cgataacgcg
     6601 ccgcgcactt gcaaccaggt ccatggcgcc gcccatacct tttaccatct tgcccgggat
     6661 catccagttg gcaaggtttc ctacttcgtc cacctccatc gcgccgagaa tgctcaggtc
     6721 tatgtgtccg ccgcgaatca tggcgaaaga gtcggcgctc gaaaaaaatg acgttcccgg
     6781 aagttcggtc accgtctctt tcccggcgtt gatgaggtcg gggtcttcct gtcctgcaat
     6841 gggatatggc ccaacgccta gtattccgtt ttcagattgc aaaactactt cgattccagg
     6901 aggaacatgg ttcgccacca gcgtaggcat ccctatgccg agatttacgt aatagccgtc
     6961 gcgcagctcg cgcgcgattc ttttgacgat acgctcacgt ttttcaactt cctgtggttt
     7021 gacggtcaat ggcgcgctgg gattcgatac agtagtcata gcggtcgttt tcggaatgcc
     7081 tccggctgtt ttcaatcttc gggcgcgttc atccttgcgc ggaactattc tagtccttgg
     7141 cggcctgatt ttgtcattcc tgctttcagg ctttcccaat acccggcctt cgattttcct
     7201 gctggtgcct acatttctcg cgctttatgg aacctacgat acggtgcgct gtctgcgttt
     7261 gcgctggagt ctttatcacg gccgtgtgct ggtgctgctg tacatggacc taatggcatt
     7321 ggcaatgatc ttcttccttt tgctttatcc ctatgaaggt tggcttcaat aacaggacgc
     7381 aaagatgcag gtatgggctt gagttttcct tatttatcag cagaagacaa gactgttact
     7441 tgtaccggga cttccagtgt gcctatacgg ccgcttagcg ggtcgtaaac tcccgtgcgc
     7501 aggaagacag cgtttttggg caaatcaagc tcctggcgaa actgcaaccc tacctgagaa
     7561 aatgcggagt aagttcccgg cttcagatgt aattggacct cctgaacagt agaattcaac
     7621 acattaccgt tctggtcgaa ggccactgcg gcgaaacgta cagtcccatg atgcagccca
     7681 tccggagtag tgctgaagga aagtgttggt tctgcaactg cataatccat ctggtagcgc
     7741 accaggccgc ctttgagtgt cttgttgtct ccaaccactg ggcccatcgc tgaaggctgc
     7801 ggattttcag gcgccatcat gactttgtag ataatctgac tgaagctggg gaggccgtgt
     7861 gccatcagcg gtaaaaatgg gtcggagggc gcagttgtcg tgtgtttgtg cgcagcttcc
     7921 gggtcttctg caaagtaacc gtgccggtat tgcaggttgt atccgctacg cgcgagcagg
     7981 acttgaatgt ggcggtagcc accatccagt ttgggactgg ttggtgtata agccagcgtg
     8041 tagtaatggg atccgttatt gatgacgcgc cgaatcgcat cctgaatgcc gttggtattg
     8101 taaatagcct cgcctccagt ctcttcagca atcgtgttca tcgtctggtg ctccgcagcg
     8161 gtctgctgaa agaatttcat atctgccttg gcaaaagccg cagcattgga agggccgcgc
     8221 cgggaggcat catacacggg tgaaaccatc agaccgcgcg catcaatagg gtagactgcg
     8281 atctggccga ggcggagcat atctgtcgtc tcgcgtatct ggtcgccata aaccgacatg
     8341 gcctgaaaag gattaggtaa atccagatcg ggattgatga tgaatgggaa agacccggaa
     8401 aaccagagaa ggttctttct gccgggaatt gaactaagat accggccaag ctccgacaga
     8461 gcatcgagcg taatgcggac acgcatgtcc gttcggaagg tctgcgtatc ggcattgaac
     8521 tgctgcattg cagaaagcgc cagcgagttg ctcattgccg gatcattctg caactgttcg
     8581 ataaagtcct cctgcgtgtg gtcagtatca agcagaggtg aagactgcgg cagtagtttg
     8641 tgcttctttc catccagcgc agccaataat tgcgaagatt cgctcgtaaa accttgcacc
     8701 atacgcaggc gcgaagccag agtgaagacg gcgatacgcg ttcctggagg aagctgcttc
     8761 agatatttca gcatctgctg gcgcacatag gcctggtcag gcatgggcgt gttcagcgca
     8821 tccagcagca acacgttcac cgaatctgtt tccggcgtga gagggaagct cgtatagaca
     8881 ttgggcggca tcgccgggag tgctgtgccc tgcgcgggag cggccccggt atgttcctga
     8941 aaataggaga tggtctgggg tttgccatct tcaagaaggc tgaagtcttc ttttttgagt
     9001 ccaggcagcg cctcgccttt tgctccggta acaactacat ccaggacgac ctggcgaacg
     9061 gtcgttttat aaacgggggc aggctgggtg gaggctgagg tttgagcaaa gataccaatc
     9121 gcaagcggag acaataggca taaagcagca atcagacgca ctcagcatct cctgtgagtg
     9181 gaaacacagc cattttgccc gaagttgtgg gcgaaggtga agaaaaatta aggctgtgtg
     9241 cccttcggag atgctatgga gattacaact ttgcgatgtt cattctccgc cggcgtactt
     9301 taccgagcat ccataaggtc gggtatagga aacggccacc cgctggcctt tcatggcttc
     9361 ggtcaaggcc tcggacacat aattttttgc cactttgata tcagccggat ctgttgtggg
     9421 atggtcgtca atggctcctt catagacgac gttgcctgaa ggatcaatga caaacatatg
     9481 tggggtggtt ttggcctcat attcatgccc gatcacgcca gatggatcga ggatggccgc
     9541 agtcggagat gctttcattt ttgtcatgta agcattctcc tcgcttgcag tcatgtatcc
     9601 ctgctggcca ggagcggagg agaggatggt gagccacact acacctctgg ccgtccattc
     9661 tttctggagc gcctgcatgt tgccgctttc gtaatgcttg cgggtgaagg ggcagtcttt
     9721 gtttgtccat tcgagaacaa caaatttgcc acggtagtca gagagcttgt g
//
LOCUS       DASRBV010000310         9608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_325591, whole genome shotgun sequence.
ACCESSION   DASRBV010000310 DASRBV010000000
VERSION     DASRBV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325591"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1699)
                     /locus_tag="VFA02_11420"
     CDS             complement(110..1699)
                     /locus_tag="VFA02_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZU10500.1"
                     /translation="MKILTADEMRACDQATSEQFSVPSLTLMENAGQAVARFVLREFP
                     HARHITVLCGKGNNGGDGFVAARALSQAGRKVKVLLLGFAADLKGDAAAMFEQMEIAP
                     ALAPDETALASEQVSELLQHADLFLDAVVGTGFKPPLRGVAAALRNRINALATPVIAV
                     DIPSGWDSDSREYQVEGAYRADAVVTFTAPKLAHVCGNLTNSASKPIVVAPIGSPDEA
                     IQSRLQLTWAGSYKKLAEAPRPAESNKGRHGHVLVIAGSYGKSGAAAMASLAALRAGA
                     GLVTAAVPQPILDSVARIAPELMTIPLRAGGHGEISSSNLDRDTLDELLDKKTVLAIG
                     PGMGQQPETEKFLLGLLENTNLPAVLDADALNVLSKHRDKISGRNRLLVLTPHPGEMA
                     RLAGLSIQQVQANREALAREFAIRHHVVLVLKGWRTLIAHPDGSVAINTTGNPGMAKG
                     GSGDILTGIIAAMVAQYPQYSDEAVNAAVYLHGLAADFAVREQDEHTLLATDTVAHLS
                     RAFRFRAEDKGGYLWIQGLPQ"
     gene            1827..2072
                     /locus_tag="VFA02_11425"
     CDS             1827..2072
                     /locus_tag="VFA02_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZU10501.1"
                     /translation="MKTYNIHHAKTHLSRLVEQAARGEPFVIAKSGKPMVKVMALEMP
                     EPSHRTRFGFLAGQIQVPDDFNTMGASEIEKMFEGED"
     gene            2069..2455
                     /locus_tag="VFA02_11430"
     CDS             2069..2455
                     /locus_tag="VFA02_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU10502.1"
                     /translation="MKLLLDTHLLLWAAMGSGRIPTRAHKCMADPVNELLFSAASLWE
                     VAIKSALRRPDFQVDVRLLRRGLLDNGYLELPVLSEHTAALDALPSIHKDPFDRILIA
                     QAIVEGVILLTNDAVMARYPAPVRLA"
     gene            complement(2468..3709)
                     /locus_tag="VFA02_11435"
     CDS             complement(2468..3709)
                     /locus_tag="VFA02_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZU10503.1"
                     /translation="MKRIAYFLLLGLLAIPVSVWAGSTTTSRHSARRSRVVESSRARQ
                     HMTRLQHTHRVAHLETASLTVRHGRVRHTSLRVHRYYERFTGNSFADMDALTAGDVTG
                     GEDPIVRQAAIDALGNMNGTVVAIDPDNGRILAMVNQKLALSSGAEPCSTIKLSVALA
                     ALDEGIVTRDTPVNLGGHYSMNLTQALAHSNNLYFETLGRRLGFERVRHYATEFGLGE
                     LAGYNIPGEHLGVYPDEPIPDSEGGVGRMCSFGEGVSMTPLQLGALVAAIANGGTLYY
                     LQHPTTPDEVLNFQPRVKRTLDISKILPEIQDGMHAAVEYGTARSLRANFNQFPILGK
                     TGTCSNHGTRYGWFASYADTQYGRIVTVFFLEGGRPTFGPKAAELTGVFYRNLWDKNY
                     FVSKPATETASAKLSSGAAAQ"
     gene            complement(3953..5263)
                     /gene="ftsZ"
                     /locus_tag="VFA02_11440"
     CDS             complement(3953..5263)
                     /gene="ftsZ"
                     /locus_tag="VFA02_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZU10504.1"
                     /translation="MNPSEEIRIHYHEQVPRGAKIKVIGVGGGGGNAVNRMIAARVEG
                     VEFIVANTDAQALQLSHAPVKIQLGLKLTSGLGAGANPDVGRRAALEDSEKIIEALEG
                     ADMVFVTAGLGGGTGTGAAPVIASLASEMGALTVAVVTRPFGFEGKRRMMQAERGMEE
                     LLESVDTMIVIPNEKLLAVAKDAGFFESFRIADDVLRQGVQGISDIITIPGIINRDFA
                     DVKTTMAGMGYAVMGTALGKGENRAVEAAQLAMSSPLLEAGAIDGAKGILINITGSSS
                     LKLSEVNEASTLIQNAAHEDANIIFGAVLDENMGDEVKITVIATGFRQEMAERRERML
                     NAALREPVSPRVPVQPVPQGSPRFMSEEEEPEILRPVIPVNVPVTAPAPVAVQPAVEP
                     APAPGPVAVAVAELPAQANTSHEAQREEQPDELDIPAFMRRGGL"
     gene            complement(5277..6494)
                     /gene="ftsA"
                     /locus_tag="VFA02_11445"
     CDS             complement(5277..6494)
                     /gene="ftsA"
                     /locus_tag="VFA02_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZU10505.1"
                     /translation="MSQKTEDLLTVLDVGSAKTRVLVAEVYDGALRYRAHGVADSKGM
                     RKGVIAELKQAARCVNDAAMMAEAMAQATIDRCVVGVGGWHVRGVNSRGGISLGTRLR
                     EITREDVRAAVDRARSVSLPNDREILHLLPQEFILDEQPGIHDPVGMIGNRLEVNLHI
                     STASASALQSIVTCANKAGLEVSESVFEAIAAAEATISADERELGVCLIDIGAGSTEL
                     VVFFEGSVAHSAVIPIGGDHFTNDLAVGLHVSVEEAEWIKCNYGHAVVTSVPSGNEIE
                     LYGMPGHGPRTVRQRFVAEVLEPRARELFQLLRDNLRQGGVLEALGAGCVLTGGGACL
                     PGLLDTAESLLRTPARIGTPVPLSRMPEELIVPENAVLVGMLLYAHRTSVTRAAEDQG
                     LRAKLRAIFAGSV"
     gene            complement(6491..7582)
                     /locus_tag="VFA02_11450"
     CDS             complement(6491..7582)
                     /locus_tag="VFA02_11450"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZU10506.1"
                     /translation="MAKIGSYQDGPAIAEHEQPDEFLRPAVKVRTRLQRPIPRMEEDG
                     PAEDEAFLRARKRVPVRKGVLPPFLTKTIPGRVVLGFSAVLVIVLGAMIVMVVRNFLD
                     HDPHFRIESATNIQIDGNSQLTRPELLSVFGADIGRNLFFVPLDQRRRELEQLPWVAH
                     ATVMRILPNQIRVSIVERVPIAFARVGNLIKLADADGVLLDMGPAALAAKHYSFPVVT
                     GINPQDPLSVRRPRMQLYQKFMNDIDTGSEKVSQQLSEIDLSDPEDVQGLVSTNGSDI
                     LLHFGQDNFLNRWNEYKEHLAEWKQQYPNLASVDLRYDRQVVLKMSKEADTVQQSPAQ
                     PVAAEQLQPAAKAARAKAHPVAHPHRKRA"
     gene            7840..8817
                     /locus_tag="VFA02_11455"
     CDS             7840..8817
                     /locus_tag="VFA02_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZU10507.1"
                     /translation="MRTFSIGVDLGGTNLRIAAFTQDWERLKAISMPTRVAAGPQAVL
                     DDMCSAIRKLVSEFEKDREFAGVGVGCPGPLALPSGRVLTPPNLPGFEGLELKTELER
                     ILECPVVVECDANAAALAECHAGAGKATGLQSLCMLTLGTGVGSGIILNGHVWHGFAG
                     MAGEAGHVPVYHDGLLCGCGSRGCLEQYASATAIARAGRKLVAAGRAGNGHDAVTARS
                     IAEAALAGDAESQEIYDEVGRALGIGLAGLVSTLNLPLYVIGGGVAAAWDLFAPAMFA
                     ALEADSYIYRLTKPADHNVPEKSKTNVVPASLGPDSGLLGAAMLPFFER"
     gene            8924..9178
                     /locus_tag="VFA02_11460"
     CDS             8924..9178
                     /locus_tag="VFA02_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10508.1"
                     /translation="MKDRRKTEPHDLEEHASTKYRPPTGVQPAPAPVEPEDAYLTGAS
                     RSLQRRLLGLNIENGQAQRHPDTPAGIHSTGSFTGKAKKS"
     gene            complement(9248..>9608)
                     /gene="rmuC"
                     /locus_tag="VFA02_11465"
     CDS             complement(9248..>9608)
                     /gene="rmuC"
                     /locus_tag="VFA02_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZU10509.1"
                     /translation="TTLIALLKAVAYGWQQLEITRNAQIIRDTGEKLYEKLATAHRYF
                     SRMGNALANAVRHYNDLIGCLEGRGSVFTHARTLHELAIGQAELPEMPRLESVTRDLK
                     ASDWKQEEVEKLFSETD"
BASE COUNT         1956 a         2676 c         2894 g         2082 t
ORIGIN      
        1 atgagaaatt tcggcggcgc tagcagtttt acaatttcgc ggccaacacc aaccgtatcg
       61 gcggcactgc tcgtttcaaa ttcgtggact cgtgttcctg tcgcctgggt cattgcggca
      121 atccttgtat ccaaagataa ccgcccttgt cttccgcgcg aaagcgaaaa gctcgtgaaa
      181 gatgggcgac ggtgtcggtg gccagcaaag tgtgctcgtc ctgctcacgg acggcgaaat
      241 cagctgcgag gccgtgcagg tagactgctg cgttgactgc ttcgtccgaa tattgcggat
      301 attgcgccac catagcggct atgatgccgg tgagaatgtc tccgctgccc cctttggcca
      361 tgcccgggtt tccggtggtg ttgatggcga cgcttccgtc gggatgggcg ataagcgtgc
      421 gccagccctt cagaacaaga acgacgtggt gcctgatggc aaactcgcgc gccagtgcct
      481 cgcgattggc ctgcacttgc tgaatgctta acccggcaag acgcgccatt tcgcctggat
      541 gcggcgtgag caccagcagg cggttacggc cgctgatctt gtcccgatgt ttcgagagga
      601 cattcagcgc gtctgcatcc agcaccgcag gaagatttgt gttttctagc aggccgagca
      661 ggaatttttc cgtttctggc tgctggccca ttccggggcc gatggcgagg actgtctttt
      721 tgtccagcag ctcatcgaga gtgtcgcggt caagattgct gctgctgatt tcgccgtgtc
      781 cacctgcgcg aagaggaatg gtcatcagtt cgggggcgat gcgcgcgact gaatcaagga
      841 tgggctgtgg gactgcggcc gtaaccagtc cggcccccgc tctgagcgca gcaagagaag
      901 ccatggctgc tgcgccggat tttccataag acccagcgat gaccagtacg tgcccatggc
      961 ggcctttgtt gctttcggcg gggcgcggcg cttctgcgag ttttttgtat gatccggccc
     1021 atgtaagttg cagcctggac tggatggctt catccggcga tccgataggc gcaacgacga
     1081 taggtttgga tgcgctgttg gtcaggttgc cgcagacgtg cgcgagttta ggtgcggtga
     1141 aggtgacaac ggcatcagcg cgataggcac cttcgacctg atattcgcgt gagtcggagt
     1201 cccatccgga agggatgtcc acggcaatca caggtgtggc gagcgcgttg atgcggttgc
     1261 gaagggcggc cgccactccg cgtaagggcg gcttaaaacc tgtgccgacg actgcatcga
     1321 gaaagaggtc agcatgttga agcagttctg agacctgttc tgatgcgagt gcggtttcgt
     1381 caggggccag cgctggggca atctccattt gctcgaacat ggccgcagca tcacctttca
     1441 aatcagcggc aaagccgagg agaagcacct tgaccttgcg tccggcctga ctcagggcgc
     1501 gggcggcgac aaagccatct ccgccattgt tgcctttgcc acagaggacg gtgatatgcc
     1561 gcgcatgagg aaattcacgc agtacaaagc gggcgactgc ctgtcctgcg ttttccatca
     1621 gagtgagcga ggggacgcta aattgctcag aagtggcctg gtcgcaggca cgcatttcgt
     1681 cagcggtgag gattttcatg gctctccacc atcattcatt cttgtaaatt cttcatatta
     1741 cgatgcttaa atatctgcta cccaatcttt gacaatagta tattttcagt tgcgaatagt
     1801 agactaagtt caatagacta agtttaatga agacttataa catccaccat gcaaagacgc
     1861 acctttccag actggtagaa caagccgcca gaggagagcc gtttgtgatt gcgaagtcgg
     1921 gcaagcccat ggtcaaagtc atggcacttg agatgccgga gccgtctcac aggacgcggt
     1981 ttggatttct ggcgggacag atccaggtac cggatgactt caataccatg ggcgcgtccg
     2041 agattgaaaa gatgttcgag ggcgaagatt gaaattactg ctggacactc atctgctgct
     2101 ctgggcggcc atgggaagcg ggcgtattcc tacccgggca cataaatgca tggctgatcc
     2161 ggtaaacgaa ctgctgttca gcgctgccag tttatgggag gtcgccatca agagcgccct
     2221 gagacgtccg gattttcagg tagacgtgcg cctgctccgg cggggactgc tggacaacgg
     2281 atatctcgaa ttaccagtcc tgagtgaaca taccgcggca ttggatgcgc taccctcaat
     2341 ccataaagat ccgttcgacc ggatactgat tgcgcaagcc attgtcgaag gggttatttt
     2401 gctgacgaat gatgcggtga tggcgcggta tcccgcgccg gtccgtttgg cgtagaaggt
     2461 ctggctgtta ttgcgctgcg gcgccggagg acagcttcgc tgaagctgtc tctgtcgcag
     2521 gcttgctgac gaagtagttc ttgtcccaca aattgcggta gaagacgccg gtcagttcgg
     2581 cggctttggg gccgaaagtc ggacgtcctc cttcgaggaa gaagacagtc acgatgcggc
     2641 catactgcgt gtcagcgtag gaggcaaacc agccgtaacg ggtgccatga ttggagcagg
     2701 tcccggtctt acccagaatc gggaactggt tgaagttcgc gcgcagcgag cgggcggtcc
     2761 cgtattccac ggcggcgtgc atgccgtcct gaatttcagg aaggatcttg gagatatcga
     2821 gggtacgctt cacgcgcggc tggaagttaa ggacttcgtc tggagtggtg gggtgctgga
     2881 gatagtagag cgttccgcca ttggcgatgg ccgccaccag cgcgcccagt tgcagggggg
     2941 tcatcgagac gccttcgcca aaggaacaca tgcggcctac gccgccctcg ctgtctggaa
     3001 tcggctcatc ggggtagact ccgagatgtt cgccaggaat gttatatcct gccagttcgc
     3061 cgagtccaaa ttcggttgcg tagtggcgga cgcgctcaaa accgagcctg cggcccaagg
     3121 tctcaaaata aagattgttg gagtgggcca gtgcctgggt aaggttcatg ctgtagtggc
     3181 cgcccaggtt cacaggggtg tccctggtaa caatgccttc gtcgagggct gcgagcgcaa
     3241 ccgaaagctt gatggtggag caaggctcgg cgccgctgga gagcgcgagc ttctggttca
     3301 ccatggccag aatgcgtcca ttgtccggat caatggcaac aaccgtgccg ttcatattac
     3361 cgagggcgtc aatggccgcc tggcggacga tcgggtcttc gccgccggtg acgtcgcccg
     3421 ccgtgagtgc atccatgtcc gcaaaagagt ttccggtgaa gcgctcataa taccgatgga
     3481 cacgcaggct ggtgtgacgt acccgtccgt ggcgtacggt aagtgaggcc gtctcaagat
     3541 gggccacacg atgagtgtgt tgcagacggg tcatgtgttg ccttgctctg gaggattcca
     3601 cgacgcgaga gcggcgtgca ctatggcggg aggtagtcgt gctaccagcc cacacggaca
     3661 cagggatcgc aagcagtccc agcagcaaaa aatatgcgat gcgcttcatt ccgtattcac
     3721 cccgaggccc agcaactgca tcgtacgcta tgaaacagcc tggaacttct gttcattcgg
     3781 catggtccgc cagtctgttc ccggaatggt catgcccttc cattctggaa cggggctact
     3841 tctatggatg caaacacatt gaatccagaa aagatgcccc ttcatctttt tgtgactttc
     3901 agataagtaa tgtgtcgggc ggataccttc actatacctg tctgaaagcg ttttacaagc
     3961 ccccacgacg cataaatgcg ggaatgtcca gttcatcggg ctgttcttcc cgctgtgctt
     4021 catgggaggt attggcctgc gcaggcagtt ccgcaaccgc aactgccact ggcccaggcg
     4081 cgggcgctgg ttctacggcc ggctggaccg cgacaggcgc aggtgctgta acaggcacgt
     4141 tgaccggaat gaccggacga aggatttccg gctcctcttc ctcgctcata aagcgtggac
     4201 taccttgtgg cacaggttgg acgggcacgc gcggcgaaac cggttcgcgc agggccgcat
     4261 tcaacatacg ctcgcggcgc tcggccatct cctggcggaa ccctgtggcg atgacagtga
     4321 ttttgacctc atcacccata ttttcatcga gtaccgcgcc gaagatgatg ttcgcatcct
     4381 catgcgcagc gttctggatg agggtcgaag cctcattgac ctcgctcagt ttgagtgaac
     4441 tggagccggt aatgttaatc aggatgccct tggccccgtc aattgcccct gcttcaagca
     4501 gcggcgagga catggccaac tgcgccgcct ctacggcgcg gttttcgcct ttgcccaggg
     4561 ccgtgcccat gaccgcatag cccatgcctg ccatggtggt ctttacatcg gcgaagtcgc
     4621 ggttgatgat acctggaatc gtaataatgt ccgagatgcc ctgcacgcct tgacgcagca
     4681 cgtcgtctgc tatgcggaag gactcgaaaa agcctgcatc tttcgctacc gcaagcagct
     4741 tttcattggg aatgacgatc atcgtgtcta ccgactcaag cagttcttcc atgccacgct
     4801 cggcctgcat catgcggcgc ttgccctcaa agccgaaggg ccgggtaact acagcgaccg
     4861 tcaacgcgcc catttcgctg gccagagagg caatgaccgg ggccgcgcca gtacccgtgc
     4921 ctccgcccag tccagccgtg acaaagacca tgtccgcgcc ctcgagtgcc tcgatgatct
     4981 tttccgaatc ttcgagagca gcgcgccggc ccacgtccgg gtttgcgccc gctccgaggc
     5041 cgctggtcag cttgagcccg agttgaattt tcaccggagc gtgggagagt tgcaacgcct
     5101 gtgcgtcagt attggcgacg ataaactcca cgccttccac gcgggcggcg atcatgcggt
     5161 tgactgcgtt gccgccgccc ccgcccacgc cgatgacctt gatcttcgcc ccgcggggca
     5221 cctgttcgtg atagtgaata cggatttctt ctgacgggtt catgggcatt tggctcctac
     5281 acgcttcctg caaaaatggc gcgcagcttg gcgcgcagtc cctggtcttc cgccgcacgg
     5341 gtcacgctgg tgcggtgggc atacaaaagc attccgacga gaacagcgtt ctcggggaca
     5401 ataagttctt ccggcatcct tgagagcggc accggtgttc cgatgcgcgc aggggtacgc
     5461 agaaggctct cggctgtatc gagaagcccg ggaaggcatg ctccgcctcc cgtcaacacg
     5521 catccggcac ccagggcctc aaggaccccg ccctggcgca ggttgtcacg cagcaactga
     5581 aagagttcac gggcgcgtgg ctcaaggact tctgccacga accgctgccg aaccgtacgg
     5641 gggccgtgac cgggcatgcc gtaaagctca atttcattgc ctgaaggcac cgaggtgacg
     5701 acggcatggc catagttaca cttgatccat tcggcctctt cgacggaaac atggaggccg
     5761 acggcaagat cgttcgtgaa gtggtccccc ccaatgggaa tgacggcgga atgggcgacg
     5821 gagccttcaa agaaaacgac cagctcggtc gagcctgctc caatgtcaat gaggcaaacg
     5881 cccagctcgc gctcatcggc ggaaattgtg gcctcagcag cggcgatggc ctcaaataca
     5941 gattcgctga cttcaagacc tgctttgttg gcgcaggtca cgatgctctg taaggcgctg
     6001 gctgaagccg tcgagatgtg cagatttact tcgagccggt tcccgatcat gccgacaggg
     6061 tcgtggatac ccggctgctc gtcgagaata aattcctgcg gcagaagatg aagaatctca
     6121 cggtcatttg gcagcgaaac ggagcgggcg cggtctacag ctgcgcgcac atcttcgcgc
     6181 gtgatttcgc gcaggcgcgt ccccaggctg atacctccac ggctgtttac gccgcgcacg
     6241 tgccacccac ctacgccaac tacgcagcgg tcaatcgttg cctgggccat ggcctcggcc
     6301 atcatagcgg cgtcattcac gcatctggcg gcctgcttca gttcggcgat cacgcccttg
     6361 cgcatccctt tggaatcggc aacgccatgg gcgcggtaac gaagcgcgcc atcgtagacc
     6421 tcggcgacca gcacacgcgt cttcgcactc cccacatcca gtactgtcag caaatcttct
     6481 gtcttctggc tcatgctctt ttcctgtgag gatgggctac ggggtgcgcc tttgctctgg
     6541 cggctttcgc tgccggctgt agttgctctg ctgcgacggg ctgggcaggg gactgctgga
     6601 ccgtgtctgc ctctttcgac atcttgagga ccacctgacg gtcatagcgc aggtctaccg
     6661 atgccagatt gggatactgc tgcttccact cggccagatg ctccttgtat tcgttccagc
     6721 ggttcagaaa attgtcctgc ccaaagtgca ggagaatgtc ggagccgttc gtggagacta
     6781 gcccctgcac atcttccggg tcggaaaggt caatttcgct caactgctgc gagacctttt
     6841 cgcttccggt atcaatgtca ttcatgaact tctgataaag ctgcatgcgc ggacgccgca
     6901 cagaaagcgg gtcctgagga ttgattcctg tcacgaccgg aaatgagtaa tgttttgctg
     6961 ccagcgcggc ggggcccatg tccagcaaaa caccatcggc atctgccagt ttgatcaggt
     7021 tgccgacgcg ggcaaaagca ataggcaccc gctccacaat ggaaacgcgg atctggttgg
     7081 gcaggatccg catcaccgtt gcatgggcca cccagggaag ttgctccagt tccctgcggc
     7141 gctgatcgag agggacaaaa aagaggttgc gtccgatgtc cgcgccgaaa acagaaagca
     7201 gctccggtcg cgtgagctgg ctgttgccgt caatctgaat gttcgtggct gactcgatgc
     7261 ggaagtgtgg atcgtgatcg agaaaattcc tgaccaccat cacgatcatg gcgcccagaa
     7321 cgatgaccag cacggcagaa aagccgagga caacgcgtcc agggatggtt ttggttaaaa
     7381 atggaggcag aacgcccttg cgtaccggga cacgcttgcg cgcgcgcagg aacgcctcgt
     7441 cctcagcagg tccgtcctct tccatacgag gaatgggccg ctggagccgc gtgcggacct
     7501 tgaccgcagg acgcagaaac tcgtccggct gttcatgttc cgcaattgcg gggccgtctt
     7561 gataggaacc gattttcgcc acgcccgagc cgagtcgtga gagtcgccat gatcactata
     7621 gcgtgccgcg tgctgtggaa agagcaaaat tccctcaggg tgaatctaag aaggcaatct
     7681 tgagacaaga cggagtttgc ccacggcaaa tgcgtattcg cgcgtcgtgg tttcgtttcg
     7741 ccaatgacag tggtgtgtaa atatatcgag ctcggcggac attacggcgc gttttagaga
     7801 acaacgatag ataacgttat catatacact gaagctgaca tgcgtacctt ctcgattggc
     7861 gttgatcttg gcggcacaaa cctgcgcatt gctgcattta ctcaggactg ggaacggctg
     7921 aaggctatct ctatgcctac gcgcgtagcc gccggaccgc aggcggtcct ggatgatatg
     7981 tgttcagcaa ttcgaaaact ggtttctgaa tttgaaaaag atcgtgaatt tgctggggtt
     8041 ggagtcggtt gtccggggcc gctggccctg ccttccggac gcgtgttgac tccgcccaat
     8101 cttcccggat ttgaagggct ggaactgaaa acggaactgg agcggatcct ggaatgcccg
     8161 gtagtcgtgg agtgcgacgc gaatgcggct gctctggccg aatgccatgc cggcgcgggg
     8221 aaagcgacag gtctccaatc actctgtatg ctgacgcttg ggaccggcgt cggtagcggc
     8281 attatcctga acgggcatgt ctggcatggc tttgccggaa tggctggcga ggccgggcac
     8341 gtgccggtgt atcacgatgg cctgctctgc ggatgtggca gccggggctg tctggaacag
     8401 tatgcttcag cgactgcgat tgcgcgtgca ggtagaaagc ttgtggccgc gggcagagcc
     8461 ggcaatggtc atgatgccgt gaccgcgcgc tcgatcgctg aggctgctct ggcgggcgat
     8521 gctgaatcgc aggaaattta tgacgaagta ggcagggcgc tcggaatcgg gctggcgggc
     8581 ctggtaagca cactgaatct tcctttatat gtgattggcg gaggggttgc cgctgcgtgg
     8641 gacttgtttg ctccggcgat gttcgcggct ctggaggcgg acagctatat ctaccgcctg
     8701 acgaagcccg cggaccacaa tgttccggaa aaatcgaaga cgaatgtggt gcctgcgagt
     8761 ctcggtccgg attcagggtt gcttggagcc gccatgctgc cgttctttga gcgctgagaa
     8821 atgaaagaca ttaccgtgaa acgcagatct ggatttttat attcagccgc gcaggcgatg
     8881 ttttatgcgc atctaactct tttgagggtt gaagaggtgc cgcatgaaag atcgcagaaa
     8941 aacagagccg catgatcttg aagaacacgc ctcaacgaaa tacagaccgc ccacaggcgt
     9001 ccagcccgcc cctgccccgg ttgagccgga agatgcttat ctgacaggcg ccagccgctc
     9061 cctgcaacgc cgcctgcttg gtttgaacat tgagaatgga caggcgcagc ggcatccgga
     9121 cacacctgcg ggaatccact cgacagggtc gttcaccgga aaagcgaaga aatcctgagc
     9181 cctatccccc gcagaagaca caacatgaag gctgaagtgg gtgaaggggt gttctccgca
     9241 ttcatcctta gtctgtttca gaaaaaagct tttccacttc ttcttgtttc cagtcgctgg
     9301 ccttcaggtc gcgcgttacg gattcaagcc tgggcatttc gggcagttcg gcctgtccga
     9361 tggccagttc atgcaatgtc cgggcatggg taaagacgct gccccgccct tccaaacacc
     9421 ctatcaggtc gttgtagtgc ctaacggcat tcgcgagggc gtttcccatc cgggagaagt
     9481 aacggtgcgc cgtcgccagc ttttcatata gcttttctcc cgtatcacgg atgatttgcg
     9541 cattcctcgt aatttcaagc tgttgccagc cgtaagcgac ggccttcagt agggcgatga
     9601 gcgtggtc
//
LOCUS       DASRBV010000311        13574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_19707,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000311 DASRBV010000000
VERSION     DASRBV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19707"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFA02_11470"
     CDS             <1..1572
                     /locus_tag="VFA02_11470"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU10510.1"
                     /translation="SVMAGQIALAFDNAFHFAELELARMRLENETARLQILLDLNNAI
                     VSDLDAATLMQEISPKMRGLMQLDAVALILPDSGDGALRLHALDFPEMKEERDVGPLH
                     QESIAAKVFRSGQPWVGNLGPMEGTGSISGLQTVCVLPLVRRNRILGVLGLGRIQKTE
                     FAQSDIDFLLQVAHQVAIAVENALAYGQVIRSNKQLAREKLYLEDEIRDHLNFKEIVG
                     ASAVLHHILRQVETVAPSETTVLICGETGTGKELVARAIHNLSAHKEAAFVKVNCAAL
                     PAGLLESELFGHEKGAFTGALTQRIGRFELANEGTIFLDEISELPLELQPKLLRVLQE
                     REYERLGSSRTLRTNARLIAATNRDLKAMIVAEKFRADLFYRLSVFPLELPPLRERPE
                     DIPLLVSHFVRYFSRNLNKQIDSVSTETMEALTGYEWPGNIRELQNVIERAVLLSDGD
                     VLRIPASELRSDKAHLFNSATMTLEEAERRHILSVLRETNWVLSGPNGAAARLGMQRS
                     TLQFRMKKLGISRPA"
     gene            1652..2083
                     /locus_tag="VFA02_11475"
     CDS             1652..2083
                     /locus_tag="VFA02_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZU10511.1"
                     /translation="MNEASVIREILDNSKTIAVVGLSDKEGRASYGVSRFMQGRGYRI
                     VPVNPLIESSLGEKAYPTLDAALAAGKIDLVNVFRAPEFVPEVVKDTIRLHIPYLWLQ
                     EGVIHDEAAQMAEKAGIKVVMDRCILKERMAAGWGGSFGAI"
     gene            2156..4267
                     /locus_tag="VFA02_11480"
     CDS             2156..4267
                     /locus_tag="VFA02_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZU10512.1"
                     /translation="MRVRNFFLLWILLLVAATSWSASTATAPHLSVQLVVPPAELYPG
                     QNFTAGLYFKLERGWHVYWTNAGDSGEPPRIQWTLPEAVSAGAMQFPTPKRLPLGPLM
                     DFGYEDEVLFPVPVQVAPGFKPPANGKAVLAAKVDWLVCREVCIPGKAQLDVGRRALS
                     APPASPSFIESDKQLITRFAGTLPKPMPAGMQAKFVPTATGFSLTVLTGKRESAAQFF
                     PFDQNQIANAAPQLITPLKNGVRIDIKKDENLQKSPEVLHGLVVLADGRAFDVQATQG
                     TLPAETVTGNGGHGAILRVVALAFVGGIILNLMPCVFPVLFIKGLSLLNSSQQERSKL
                     RAHGAVYTLGILVSFWIVVALLLLLRAGGRQLGWGFQFQSPGFVAMMALLLFFLGLSL
                     AGMFEIGLSLTSAGSGLAAKHGYSGSFFTGVLAVIVATPCTAPFMGAAIGYALAHSSV
                     VSFLVFTALALGLAAPYLLLAFQPAWTRILPRPGAWMEVLKQATSVPVFGTVIWLVWL
                     LAQTAGPNALVGLLSAFVLLAIAGWILGRWPAKVPATAAATLVVACAVALSLFSVRRL
                     GAASGSAAPIASSEWQPFTPDIVDKYRQQGRPVFVDFTAKWCLSCQVNERVILERADV
                     QQKFRSGNFALIRADWTNSDEAITKALNALGRSGVPTYAVYSGSPQEGPQVLPEVLTP
                     GVVLDALDKAQQQTQALRSRP"
     gene            4333..5700
                     /gene="fliD"
                     /locus_tag="VFA02_11485"
     CDS             4333..5700
                     /gene="fliD"
                     /locus_tag="VFA02_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZU10513.1"
                     /translation="MGTVGLSFGSPTSGQGFDVTSTVSQIVTNMQAVETPWKNQLSAL
                     QSQDTALSSIGSDLSSLSSALQSLTDFAGVLSEKQGSSSDTSVLQLTSASTSAIAGSH
                     TITVQGLATTSSYVSSTVSGSSDILTGSLTVNGQTINIDSTNNTLSSLAGAINSGSYG
                     VIANVITDSSGSRLSLVSKTSGASGQISVSSSLVDSSNGSSAVSFTQTQAGTDAQLTV
                     DGVSISSPSNTVTNAIQGVTFQLLAPSSNGEQVQVVITNSNSAVESAINSFVSAYNKV
                     VQDLNTQEGKDASGNPEPLYGSPTLTSLQEALQSALNFTQTSGAITSLSQLGMTVGSD
                     GTLSVNSDTLDSVLNTNYQDVVDFFQPSGTFTSFGGNLTSVLNNLSSSSSNGAIKLAL
                     QQNAAVEQQLNQNISNEETIISAKKTELTAQLNEANYILTSIPQQLQQIDEIYSAITG
                     YNQKQ"
     gene            5714..6145
                     /locus_tag="VFA02_11490"
     CDS             5714..6145
                     /locus_tag="VFA02_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZU10514.1"
                     /translation="MQNRTYQQQSLAGMNPVELIVALYDGMVRFLNQAIAATEAGDIR
                     ARRIAVKRVLDILMHLQSRLRMDVGGPAKSLSEFYAAIFALCLEGSRLSSPERFQEAV
                     RNVLSVREAWQQVAQDPEVRAMYTPPLAPRAEAAQVSGWTV"
     gene            complement(6142..7908)
                     /locus_tag="VFA02_11495"
     CDS             complement(6142..7908)
                     /locus_tag="VFA02_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU10515.1"
                     /translation="MLWKLQPLFPYLRRYRRGFLWGGVSVVLSNLIWIFFPQVIRIAI
                     DDLNHGVTERKIWLYASLLVGVSLAKGVFLFLTRWIIIGLSRDIEFDLRNDLFLQLEK
                     QSAAYYQQHRTGDIMARMTNDLNAVRMLLGPAIMYSANTLLFSIGALYFLIKISPFLT
                     LVALVPMPLASVLVQTMGRKIHERFERIQAMYSDISAQAQENFSGARLVRAFAQEEAQ
                     IAAFEKSNQEYIRRGLRLVQLMGMLWPTLEFILGLALAITLLVGGHEVLSHRISVGDF
                     VAFNTYMLMLTWPVIALGWVVNLVQRGTASVVRIDELLKAKPAIDDRDADCSIPEGRK
                     LRGEIEFRNLSFSYESESGRPVEVLHNISLKVPAGSSLALVGPTGSGKTTLANLIARI
                     YDAEPGSILVDGRPIREYPLDVLRANIGFVPQETFLFSETIRENIAFGVPHASEDEVL
                     RAAEIAHIRKEFEEFPRGFETVVGERGVTLSGGQKQRSSLARAILRNPGILILDDALA
                     SVDTYTEERILEGLQHVMEGRTTILISHRISTVRHADQIAVLMNGRIAELGTHEELLA
                     HNGHYANLFQKQLLEEELAVTH"
     gene            complement(7960..10275)
                     /gene="lptG"
                     /locus_tag="VFA02_11500"
     CDS             complement(7960..10275)
                     /gene="lptG"
                     /locus_tag="VFA02_11500"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZU10516.1"
                     /translation="MRILTRYILREVLSHAFLGGVLFTFVLFMKELGPLVELAVRNTA
                     SIGTVVKVFLLMMPNMLTVTIPMAVLVGVLLGLSRLAADSEITAMRASGIGIWRFVGV
                     VAVVATCAWGAGMANSLYFAPKASARLLQLEDELKNTQATYEIQPRVFYEDLKNAVLY
                     VEDVRAGTGASNWHQVFLADLTDPVSPKVTTAAEATVVSTDNQTLTMRMRNGAQHELS
                     TGKPDGYTVSTFGQTDMHLEAASAPDPHLGHSDAPVLAMSNRELLERAHKGRWYQIEM
                     QKRFAYPAACLVLMLVGVPLGISSKRGGKSAGFVLTILLVFVYYFLSSTGTALARQNK
                     LPVFPGVWGANLLFGICGIFFLRQMSTGGAAISAVASIGPWLRLKKASAPSEPAAPSE
                     KTVARRHVRGRFPLILDEYILRQFTGTFLMVLVAFVMLMLVFTFFELLGDIIRNQTPL
                     ITVGEYLINLTPNMLYNLTPLSVLIAVLVVFGLLNRTNEITAMKATGISLYRIIVPVL
                     VIASVLAFALFAFDEVYLPSANRKQEELRNIIKGKPAQTYQNLDRKWMFGKQQRGKPG
                     RIFYYEFFDAYNDRFANITVFEFDPATFSLTHRIFAASAHWDPQFNRWIFEHGWERSF
                     NGDEVSDYKTFEVSTFPEIIEPPSYFKQELRPSQEMSFNELRRYIQSLRQSGFDTMRL
                     RVQLNRKMAYPLITLIMAILAIPFALSMGRRGSLAGIAVAIGMAIAYLFVDGTFEAMG
                     NVNLLPAFLAAWSPDILFGLAGSYLLLRTHT"
     gene            complement(10517..10849)
                     /locus_tag="VFA02_11505"
     CDS             complement(10517..10849)
                     /locus_tag="VFA02_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZU10517.1"
                     /translation="MNKKQTNAHCACGDERKAVKVDPEIKAATLRRLARIAGQIRGIQ
                     KMVEDERYCADVLVQISSVQEALRGASRHLMRNHLMHCATDAIRKGPESAEMMYDELL
                     DLVYKHSR"
     gene            complement(10846..13077)
                     /locus_tag="VFA02_11510"
     CDS             complement(10846..13077)
                     /locus_tag="VFA02_11510"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZU10518.1"
                     /translation="MAGSVKTVTVPVMGMSCATCQVHIEKALRETPGVSEAQVNLLAH
                     KARVTYNPAIAQPEHLIHAIRKAGYDAALPEEHHHHEEDAGSEATLKLRAFSTLIAGV
                     IAMVLSMPLMHHAMFASLLPWLYRISHAALEYTLLAMTLAGIFWAGLPIYRGAWRATL
                     HGASNMNTLVSLGTVAAFLYSALATLAPQLFLKHGLHPDVYYESVLLILGFLLLGKWL
                     EARAKHRTLDALELFAKMQPQTARLLRNGAESEVPLAEVRPGDTLAVRPGERIPVDGV
                     ILEGTSSIDESLITGESVPVVRSKGDRIIGGSVNFDGAFIYRATSVGADSVLGQMLRL
                     MEQAQSSKAPMQQLADRVSAIFVPTVLIIAVLTFIVWAWIGSAADFSRAFAISIAVLV
                     IACPCAMGLAVPAALTVAIGRGAQLGILFKGGESVERLAHVDTVVLDKTGTLTLGKPE
                     ITAIHPANGWDERQLLAVAAALEQSSEHPLAHAVMVHARSEGIQPAPATELRAIPGKG
                     IAGTINGSSAAAGNAALMREHDITISSNGTQTATLLHIAVDGVYRGHLEAQDALRPGA
                     KEAIASLQALGMKTIMLTGDNQKAAEKVAREAGLNEVRAELLPEGKLAAIRELQQKGC
                     KVAMAGDGINDAAALAQADAGLAIGTGTDLAREAGDAILLHGDPRQIVGAIRLARQTL
                     RVMRQNLVWAMGYNLIGIPIAAGVLYPALGILLSPVIASAAMAMSSVSVLSNSLRLKG
                     FQA"
     gene            complement(13080..13286)
                     /locus_tag="VFA02_11515"
     CDS             complement(13080..13286)
                     /locus_tag="VFA02_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZU10519.1"
                     /translation="MREVTLRIDGMHCGACIRRVTQALQKIQNAEVKEVRVGAAQVLV
                     PETVPGSAVVEALAASGYSAHLEE"
     gene            complement(13334..>13574)
                     /locus_tag="VFA02_11520"
     CDS             complement(13334..>13574)
                     /locus_tag="VFA02_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU10520.1"
                     /translation="IHLTVMEAEFLRHLVQNPGRIVSRKELLEQVWHVREDTDTRAID
                     NFVVRLRRYIEDEPANPVYLKTVRGIGYRFTPHSE"
BASE COUNT         2964 a         3705 c         3955 g         2950 t
ORIGIN      
        1 tctgtgatgg cgggacagat tgcgctggct tttgacaatg ccttccattt tgcagaactt
       61 gagcttgcgc gcatgcgact ggagaatgag acggcccggc tgcagatcct acttgacctg
      121 aacaacgcca ttgtttctga cctggatgcg gccaccctga tgcaggagat ttctcccaag
      181 atgcgcgggc ttatgcagtt ggacgcggtt gcgctcattc tccctgattc aggcgatggc
      241 gctttgcggc tccatgctct ggattttccg gagatgaaag aggaaagaga cgtcgggccg
      301 ctgcatcagg aatccatcgc cgcgaaggtc ttccgttccg ggcagccgtg ggtaggtaat
      361 ctggggccaa tggagggtac cggctccata agcgggttgc agacagtgtg tgtgcttccg
      421 ctggtccggc gcaatcgcat tcttggtgtg ttgggcctgg gcaggataca gaagactgaa
      481 tttgcgcaga gtgatattga ttttttgttg caggtagcgc atcaggtggc gattgcagtt
      541 gaaaatgcgc tggcttacgg ccaggtgatt cgttccaaca aacaactcgc cagagaaaaa
      601 ctctatctgg aagatgagat tcgtgaccat ctgaacttta aagagattgt gggtgccagc
      661 gctgtgctgc accatatcct gcgacaggtg gagacggttg caccgtcgga aacaacagta
      721 ctgatttgcg gggaaaccgg aacgggcaag gaactcgtcg cccgggccat ccataacctg
      781 agcgcgcaca aagaggccgc ctttgtaaag gtgaactgcg ccgccctgcc ggcaggactg
      841 ctggaaagtg agcttttcgg tcatgaaaaa ggggctttca cgggtgctct tacgcaacgt
      901 atcgggaggt ttgagctggc gaatgaaggg acgatatttt tggatgagat cagcgaactg
      961 ccgctggagc tacagccgaa gctgctgcgc gttttgcagg agcgagaata cgagcggctg
     1021 ggcagctcgc gcacgctgcg cacaaatgcc cgcctgattg cggccacaaa ccgcgacctg
     1081 aaggccatga ttgtggcaga aaagttccgc gcagacctct tctatcggtt gagcgtcttc
     1141 ccgttggagc ttcctccgct gcgcgagcgg ccggaagaca ttccgctgct ggtaagtcat
     1201 tttgtgcgct acttctcgcg caatctgaat aaacagattg attctgtttc cactgaaacg
     1261 atggaggcgc tcactggcta tgaatggccg gggaacattc gcgagctgca aaacgtgatt
     1321 gagcgcgcgg tgctgctgtc tgacggagat gtgctcagaa taccggcatc agagcttcgc
     1381 tccgataagg cgcatctttt caacagcgcg acaatgacac tggaagaagc agaacggaga
     1441 catattttgt ctgttctcag ggagacgaac tgggtccttt caggtccgaa tggggccgcg
     1501 gcgcggctcg gaatgcagcg gtctacgctt cagttccgga tgaagaaact ggggatttcc
     1561 cggcccgcct gaaggagtga atcatgaatt cgcggcatga tcagcaccca ttcttagctt
     1621 gaagactatg tttgtggcca taataaagtg aatgaatgaa gcttctgtaa ttcgagaaat
     1681 tctggacaac tcaaaaacga tagccgttgt gggcctttct gataaagaag ggcgtgccag
     1741 ctatggagtt tcgcgcttta tgcagggacg ggggtaccgc attgtccccg tgaatccttt
     1801 gattgaaagc tctctgggtg aaaaggcata tcccacactc gatgctgcgc ttgccgccgg
     1861 aaaaattgac ctagtgaacg tcttccgcgc tccggaattt gttcctgaag tggtgaagga
     1921 caccattcgc cttcacatcc catatttatg gctgcaggaa ggcgtgattc acgacgaagc
     1981 agcgcagatg gccgagaaag ccggcattaa ggtcgtgatg gatcgctgca tcctgaaaga
     2041 aagaatggcg gcaggctggg gaggctcctt tggcgcaatc taaatcaaca acggttctgc
     2101 atgtttatct ggtaacttcg cgcatcctcg ctccgtccta caatgaaaga cagacatgcg
     2161 cgtccgaaac ttctttctgc tttggatact gctgctggta gcggcaacca gctggtctgc
     2221 ctctacggcc accgctccgc atctcagcgt gcaactggtt gtgcctccgg cagagttgta
     2281 tccgggacaa aacttcacag cgggccttta tttcaaactg gagcgcggat ggcacgtcta
     2341 ctggaccaat gccggggatt caggcgagcc tccgcgcatt caatggacgc tacctgaagc
     2401 ggtaagcgcg ggtgcgatgc agtttcccac gccgaaacgc ctgccgcttg ggccgctgat
     2461 ggatttcgga tatgaggatg aagtgctctt tcctgttccc gtccaagttg cgcccggctt
     2521 taagccgccc gcgaatggca aggccgttct ggccgcaaag gtggactggc tggtctgccg
     2581 cgaggtctgc attccgggta aagcgcagct tgatgtgggg cgcagggcgc tgtctgcccc
     2641 tcctgcctcg ccatctttta tagagagcga taaacagctt atcacgcgct ttgcaggtac
     2701 tctgccgaag ccaatgcctg cgggaatgca ggcgaagttt gttcccacag cgacaggctt
     2761 ttccctcact gtgctgacag ggaagcgcga aagcgcagcg caattctttc cattcgatca
     2821 gaaccagatt gcgaacgccg ccccgcagct catcactcct ttaaaaaacg gtgtgcgcat
     2881 tgacattaaa aaagatgaga acctgcaaaa gtctccggag gtgttgcatg gccttgtcgt
     2941 tcttgccgat ggccgcgcgt ttgatgttca ggcaacgcag ggaacgcttc cggcagaaac
     3001 cgtgaccggg aacggcggcc acggggccat cctgcgcgtc gtggcgctgg catttgttgg
     3061 cggcatcatt ctcaatctga tgccgtgcgt atttcctgtg ctgtttatta aaggactgtc
     3121 gctgctcaac tcatcgcagc aggagcgtag caaactgcgc gcgcacggag ctgtatacac
     3181 gcttggcatt ctggtgtcgt tctggattgt tgttgcgctc ctgctgctac tgcgtgctgg
     3241 tggacggcag ctcgggtggg gcttccagtt ccagtccccc ggatttgtcg caatgatggc
     3301 gctgctgctg tttttcctgg ggctttccct tgccgggatg tttgaaatag gcctttcgct
     3361 caccagcgct ggctcgggcc tggcggcgaa gcatggctat tcgggcagct tttttaccgg
     3421 agtgctggcc gtcattgtgg ctactccatg cacagcgcca tttatgggcg cggccatcgg
     3481 atacgcgctg gcgcactcat ctgtggtaag ttttctggtc tttacggcgc ttgcgctggg
     3541 actggcagcg ccgtatcttt tgctggcatt tcaacccgcg tggacgcgta ttctgccgcg
     3601 tccgggcgca tggatggagg ttcttaagca ggccacctcg gttcccgtat ttggaacggt
     3661 catctggctg gtgtggctcc tggcccagac tgcgggaccc aatgcgcttg tgggcctgct
     3721 gagcgctttc gttctgctgg ctatcgccgg atggatccta ggccgctggc cggcaaaggt
     3781 tccggcaacc gcggcagcga cgcttgttgt ggcctgcgca gtggcgttgt cacttttctc
     3841 ggtgcgcaga ctcggcgctg cttcggggtc cgcggcccca atcgctagtt cggaatggca
     3901 gccattcacg ccggacattg tagacaagta ccgtcagcaa ggcaggccag tcttcgttga
     3961 tttcacggcc aagtggtgcc taagttgcca agtgaatgag cgcgtcattc tggagcgcgc
     4021 cgacgtgcag cagaagttcc gcagcggcaa ttttgccctg atccgcgccg actggaccaa
     4081 cagcgacgag gctattacaa aagcactgaa cgcgctggga cggagtggag tgcccacata
     4141 tgcggtctat tccggatcgc cgcaggaagg gccgcaggtt cttccggaag tgctgactcc
     4201 gggcgttgtg ctggatgcgc tcgataaagc gcagcagcag acgcaggccc tccgctcccg
     4261 gccttgataa ccgtggagcc gcactgaaag ggccacgttc aagtttttcc tgatagcgcc
     4321 gataccacgg gcatgggaac agtcggcctt tcatttggat cacccaccag cgggcagggc
     4381 tttgatgtaa cttccacggt cagccagatt gtgacgaaca tgcaggcggt ggagacgccg
     4441 tggaaaaacc agctttccgc gttgcagtcg caggacacgg ccctgagcag cattggaagc
     4501 gacctcagct cgctttcctc agcattgcag tcgcttacag atttcgcggg cgtgctgtcg
     4561 gaaaaacagg gttccagctc tgacacgagc gtcttgcagt tgacctcggc ttcgacatct
     4621 gccattgccg gaagtcacac gatcacagtg cagggtctgg cgacgacgtc ctcttatgta
     4681 agcagcacgg tttccggttc cagcgatatc ctgaccggaa gcctgacggt caatggacag
     4741 acgatcaaca ttgattcgac gaacaatacg ctttccagtc tggcgggcgc aatcaattcc
     4801 ggaagctacg gcgtgattgc gaacgtgatt acagattctt ccggatcgcg gctctctctg
     4861 gtgagcaaga ccagcggcgc gagcgggcag atttccgttt ccagcagcct ggtggatagc
     4921 agcaacgggt cttcggcggt cagctttact cagacacagg ctggcaccga tgcgcaactg
     4981 accgtagatg gcgtgagtat ctcgagcccg tcgaacacgg tgacgaatgc tatccagggc
     5041 gtgaccttcc agttgctggc tccctcttcg aatggggagc aggtgcaggt cgttattacg
     5101 aacagcaatt ccgcggtaga atcggccatc aattcatttg tatcggcgta caacaaggtc
     5161 gtgcaggacc tgaacacgca ggaaggcaag gatgcaagcg gcaatcccga gccactgtat
     5221 ggcagtccga cactgacatc gttgcaggag gcgctgcaat cggcgttaaa ctttacgcag
     5281 acttccggcg cgattacgtc cttgtcgcag ctaggtatga ctgtgggcag cgatggcaca
     5341 ctctctgtca acagcgacac tctcgacagc gtgcttaaca ccaattatca ggatgtggtg
     5401 gactttttcc agccgagcgg gacgtttact tcctttggcg ggaacctgac ttctgtgctg
     5461 aataatctga gcagcagctc cagcaatggc gccatcaagc ttgctctcca gcagaatgcg
     5521 gccgtggagc agcagttgaa ccagaacatc agcaatgaag agaccattat cagcgccaaa
     5581 aaaacagagt tgacggccca gttgaatgag gcaaattaca tcctgacctc gattccacaa
     5641 cagttgcagc agattgatga aatttacagc gccatcaccg gctacaacca gaagcagtaa
     5701 ggataggggc accatgcaga acagaactta tcagcagcag tccctggcag ggatgaatcc
     5761 ggtggagttg attgtcgcgc tttatgacgg gatggtgcgc tttctgaatc aggccattgc
     5821 agccactgaa gcgggagaca ttcgtgctcg gcgcattgcg gtgaagcggg tgctcgatat
     5881 cctgatgcac ctgcaatcgc ggctgcgtat ggatgtgggt ggtccagcaa agtcgctctc
     5941 agagttttac gccgccattt ttgctttgtg ccttgaaggg tcccgtttgt cttcgcctga
     6001 gcggtttcag gaggccgtgc ggaacgttct gagcgtgcgc gaggcgtggc agcaggtggc
     6061 gcaggacccg gaggtccggg cgatgtatac tcctcctctg gccccacgtg cagaggcggc
     6121 gcaggtttcc ggctggacgg tttaatgtgt tacagcaagt tcttcttcga gaagctgttt
     6181 ctggaagagg tttgcgtaat gtccgttgtg ggccagcagt tcttcatgtg tgccaagctc
     6241 cgcaatgcgg ccgttcataa gaactgcaat ctggtcagcg tggcgcacgg ttgagatgcg
     6301 gtgggagatg aggattgtcg tgcggccttc cattacatgc tggaggcctt cgaggatccg
     6361 ttcttcggta taggtgtcca cgctggcgag ggcatcgtca agaatcagta tgcctggatt
     6421 gcgcagaatg gcgcgcgcca acgatgaacg ctgcttctgg ccaccggaaa gagtgactcc
     6481 gcgctcgccc accacagttt caaaaccacg cggaaactcc tcaaattcct tgcgaatatg
     6541 ggcgatttct gccgcgcgca gcacttcgtc ttcactcgca tgtggtacac caaaagcgat
     6601 gttctcccgg atggtttcgc tgaaaagaaa tgtctcctgc ggaacaaagc caatgttcgc
     6661 ccgcagaaca tcgagcggat actcacgaat gggacgccca tccaccagga tactgcccgg
     6721 ttcggcatca tagatacgcg caatcaggtt tgccagagtt gtctttcctg accctgtagg
     6781 ccccaccaaa gccaggctgc tgcccgcggg aactttgaga gagatattgt gcaggacctc
     6841 gaccggacgt ccactctcac tttcgtaaga gaagctgaga ttgcgaaact cgatttcccc
     6901 gcgcagcttc cggccttcag gaatggagca atccgcatcg cggtcgtcaa ttgccggctt
     6961 tgctttcagc aattcatcaa tgcgaacgac ggatgctgtc ccgcgctgga caagattcac
     7021 aacccatcca agtgcgatga caggccaggt cagcatcagc atgtacgtgt taaaggccac
     7081 aaaatcgcct acgctgatgc ggtgcgaaag cacctcgtgt ccgcccacga gcaacgtgat
     7141 ggccagagca aggcccagaa taaattccag agtaggccac aacatgccca ttaactgcac
     7201 gagccgcagt ccgcgccgga tatactcctg gttcgacttc tcaaaagcgg cgatctgggc
     7261 ttcttcctgg gcaaatgcgc gaaccaggcg cgctccggag aagttttcct gcgcctgtgc
     7321 cgagatatcg gaatacatcg cctggatacg ctcaaagcgc tcatggatct tacgccccat
     7381 ggtttgcacc agaacgcttg ccagaggcat cggcaccagc gcaaccaacg tcaggaatgg
     7441 gctgatttta atcagaaaat acagcgcgcc gatcgagaaa agcagcgtat tggcgctata
     7501 catgattgcc ggccccagca acatgcggac agcattgagg tcgttcgtca tgcgcgccat
     7561 aatgtcgccg gtgcgatgct gctggtaata agcagcggat tgcttttcca gctgcagaaa
     7621 aaggtcattg cgcaggtcga attcgatgtc gcgcgagagt ccgatgatga tccagcgggt
     7681 cagaaagaga aacacacctt ttgcaagcga gacgcctaca agcaggctgg catagagcca
     7741 gatttttctt tccgtcactc cgtgattcag atcgtcaatg gcaatgcgga tgacctgcgg
     7801 aaagaagatc cagatgaggt tactgaggac aacggaaact cccccccaga ggaatcctct
     7861 gcgatagcgg cggagataag ggaacagggg ctggagtttc caaagcatgt gttaattcta
     7921 gttctggaaa tctgattccc acagagcaga aaagtcgcat caagtgtgcg ttcttaacag
     7981 taaatagctt cccgcaaggc caaagagaat gtccggcgac caggcagcca ggaatgcagg
     8041 taacaggttg acgttgccca tggcctcaaa ggtcccgtct acgaatagat aggcaatggc
     8101 catgccaatg gcaacagcga ttccggcaag tgagcctcgc cttcccatag ataaagcaaa
     8161 gggaatcgcg agaatggcca tgatcagcgt aatcagcgga taagccatct tgcggttgag
     8221 ctggacgcgc aggcgcatgg tgtcgaaccc gctttggcgc agcgattgaa tatagcgccg
     8281 caactcatta aagctcattt cctgtgaagg acgcagttcc tgcttaaagt aagacggcgg
     8341 ttcaatgatt tcaggaaatg tgcttacttc aaatgtctta tagtcgctga cttcatctcc
     8401 attgaagctg cgctcccatc catgctcgaa gatccagcgg ttgaactgcg ggtcccaatg
     8461 cgcgcttgcg gcaaagatac ggtgcgtgag cgagaatgtt gctgggtcga actcaaagac
     8521 ggtgatgttg gcgaacctgt cattgtatgc gtcaaagaat tcgtaataaa agatgcgccc
     8581 cggtttgccg cgctgctgct tgccgaacat ccatttgcgg tcgaggtttt gatacgtctg
     8641 tgccggcttg cccttgatga tgttgcgcaa ctcctcctgc ttgcggttgg ccgagggcag
     8701 gtagacctca tcaaatgcaa agagcgcaaa cgcaagcacc gatgcaatca ccagcaccgg
     8761 gacaatgatg cggtacaggc tgatgcctgt ggccttcatc gctgtaattt cgttggtgcg
     8821 gttcagcagc ccgaatacaa cgagcaccgc aatcagcacg ctcagcggcg tgagattgta
     8881 gagcatgttt ggcgtgagat tgatcaggta ttcgcccacg gtaatcaacg gcgtctgatt
     8941 gcggatgata tcgccaagca gctcaaagaa ggtgaagacc agcatcagca ttacgaatgc
     9001 gaccagcacc atgagaaatg tgccggtaaa ctgccggaga atgtattcgt cgagaatgag
     9061 cgggaagcgc ccgcgcacat gacggcgcgc gactgttttt tctgacggag cagccggttc
     9121 tgacggagca gatgctttct tcagcctgag ccaggggccg atagaagcca cagcgctgat
     9181 ggctgcacct ccggtggaca tctgccgcag aaaaaagatg ccgcatatgc caaagagcag
     9241 gttcgctccc catactccgg gaaagaccgg caatttattc tggcgggcca gggccgttcc
     9301 ggtcgaagag aggaaatagt agacaaagac cagcagtatc gtaagcacga accccgcgct
     9361 tttgccgccg cgctttgagg agatgccgag aggcacgcca accagcatca acaccaggca
     9421 cgctgccgga taagcgaacc gcttctgcat ctcaatctgg taccacctac ctttatgggc
     9481 gcgttcaagc aattcgcggt tggacatcgc cagcactgga gcatcgctgt gccccaggtg
     9541 tggatcgggc gcggatgcag cctcaaggtg catgtctgtc tggccgaaag ttgagacggt
     9601 gtatccgtca ggcttgcccg tcgaaagctc gtgctgtgct ccattgcgca tgcgcatggt
     9661 aagggtctgg ttgtccgtgc tgacgacagt ggcctccgcc gccgtggtca cttttggtga
     9721 aacggggtcg gtaagatcag ccagaaatac ctggtgccag ttggatgctc cggtgcccgc
     9781 gcggacatct tctacgtaaa gcacggcgtt tttcaggtct tcataaaata cacgtggctg
     9841 gatttcatat gtggcctgcg tgttcttcag ctcatcttcc agttgcagca ggcgcgccga
     9901 tgcttttggc gcgaagtaca ggctgtttgc cattcctgcg ccccaggcgc aggtggccac
     9961 cactgcaaca actccaacaa accgccatat tcctattccg gatgcgcgca tagcagtgat
    10021 ttcactgtcg gctgcaagac ggctgaggcc caacagaaca cccaccagca ccgccatggg
    10081 aatggtgaca gtgagcatgt tcggcatcat aagcaggaag actttaacca ctgtgccgat
    10141 ggaggcggta ttacggaccg caagctccac taacggcccc agttccttca tgaacaggac
    10201 aaaggtgaag agcacgccgc caagaaaggc gtgtgaaagc acttcgcgca gaatataacg
    10261 ggtgagaatg cgcacggcaa aaagacttcc agctttcagt ttacagcgcg ggcggatgaa
    10321 caccacccgg gaagtatttt gctgggctgc tttatagcat cttcatatgg agagatatgg
    10381 agagccccgt cattctgacg accgaagaaa gaagaatcta gcgatgtgag gctttatcca
    10441 atcctgctaa gaattcccat gccctctcgg aatgacgatc gggcctttcg tgcatcgcag
    10501 tgaaacgaaa aatgctttag cgcgaatgct tgtagaccag gtcgagcaac tcgtcataca
    10561 tcatttcggc gctttccggg ccttttctga tcgcgtcagt cgcgcagtgc atcaggtgat
    10621 tgcgcatcag atggcggctg gccccgcgca gggcctcctg tacagaggaa atctgtacaa
    10681 ggacatcggc gcagtatcgc tcatcctcca ccattttctg gatgccgcgg atctgccctg
    10741 cgatgcgcgc cagacgacgc aaagttgcgg ccttgatttc cgggtctact ttgacggcct
    10801 tgcgctcgtc tccacaggca cagtgcgcgt tagtttgttt cttattcatg cctggaaacc
    10861 tttcaatcgt aagctgttgc tcaatacact caccgaactc atggccatgg ctgcgctggc
    10921 gatgacgggg ctgagcagga tgcccagcgc aggatacaga acacctgccg caatcgggat
    10981 gccgatgagg ttgtagccca tggcccagac caaattctgg cgcatcacgc gcagcgtttg
    11041 cctggcgagg cgaatggccc cgacaatctg gcgcggatcg ccgtgcagaa gaatggcgtc
    11101 tccggcctcg cgcgcgagat cggtgcctgt tccgatggcg agaccagcat cggcctgcgc
    11161 cagcgcagcg gcatcgttaa tgccatcgcc tgccatggca accttgcacc ccttctgttg
    11221 caattcacgg atggcagcga gcttgccttc cggaagcaac tctgcccgga cttcgttgag
    11281 tccggcctcg cgggcgactt tctctgccgc cttctggtta tctccggtga gcatgatggt
    11341 cttcataccg agcgcttgga gagatgcaat ggcctccttc gcgccagggc ggagcgcatc
    11401 ctgtgcttcc agatggccgc gataaacacc atctacagcg atgtgcagaa gcgtcgcggt
    11461 ctgcgtgccg ttagatgaaa tggtgatgtc atgctcacgc atcagcgcgg catttcctgc
    11521 tgcggctgaa gagccgttta tagtgcctgc gatgccttta ccgggaatgg cgcgcagctc
    11581 cgtggctggc gctggctgaa tcccttcaga cctcgcgtgc accatgacgg catgtgccag
    11641 aggatgctcc gacgattgct ccagcgctgc agccacggca agaagctgac gctcgtccca
    11701 tccgttggca gggtgaatgg ctgtgatttc tggtttgccc agagtgagtg tcccggtctt
    11761 gtcgagcacg acagtatcta catgcgcgag gcgctccacg ctttctccgc ctttgaaaag
    11821 aatgccaagc tgcgcgccgc gcccgatggc cacagtcagt gcagcgggaa cggcaaggcc
    11881 catcgcgcag gggcaggcaa ttacaagcac agcaatggaa atcgcaaagg cgcggctgaa
    11941 gtctgctgcc gaaccaatcc aggcccagac aatgaaggta aggactgcga tgataagcac
    12001 cgtgggcaca aagatagcgc taacccggtc ggcgagttgt tgcatcgggg ccttggaaga
    12061 ctgcgcctgc tccatcaggc gcagcatctg ccccagcacg ctgtcggctc cgacggatgt
    12121 tgcgcgatag ataaatgcgc cgtcgaaatt aacagagcct ccgatgatgc ggtcgccttt
    12181 gcttcggaca acagggacag actcgcctgt gataagcgac tcgtctatgc tgctcgtgcc
    12241 ttcgagaata acgccgtcca cgggaatgcg ctcaccagga cggacggcaa gcgtgtcgcc
    12301 ggggcggacc tcagcgagcg gaacttctga ttctgcgccg ttacgcagta gacgcgctgt
    12361 ctgcggctgc attttggcga acagttcgag cgcgtcgagg gttcgatgtt tagcgcgcgc
    12421 ctcaagccac ttgccaagca gcaggaagcc cagaatcagc aggaccgatt cgtagtagac
    12481 gtcaggatgc agcccatgct tcagaaagag ctgcggggcc agcgtggcga gcgccgaata
    12541 gagaaatgcc gccaccgtgc cgagagagac cagcgtgttc atgttgctgg cgccgtgcag
    12601 tgtcgccctc catgccccgc gataaatggg cagacccgcc cagaatatgc cagcaagcgt
    12661 catcgccagc agcgtgtatt ccagcgctgc gtgcgaaata cgatagagcc agggcagcaa
    12721 actggcaaac attgcatggt gcatcagcgg cattgagagc accatagcaa tgacgccggc
    12781 gatgagggtc gaaaaggccc gaagctttag agtggcttca ctacctgcgt cttcttcgtg
    12841 atgatgatgt tcttccggca gggccgcgtc atagcctgcc ttacggatgg catgaatcag
    12901 gtgctcaggc tgcgcaatgg ccggattgta agtaacccgc gccttgtggg ccagcagatt
    12961 cacctgcgct tcgctcacgc ctggggtttc gcgcagcgcc ttttcaatat gcacttggca
    13021 ggtagcacag ctcatgccca tcactggcac cgtaacggtt ttgacagatc cggccatggt
    13081 cactcctcca gatgcgcaga atagccggag gccgcgaggg cctcaacaac cgcggaaccg
    13141 gggacggttt ccggcacaag gacctgcgct gctcccaccc gtacctcttt cacctctgca
    13201 ttttggatct tctgcagtgc ctgcgtgacc ctgcgaatac atgctccgca gtgcattcca
    13261 tcaatgcgaa gtgtaacctc tctcatagat atacccccta tgcctattat gccacaatgc
    13321 atgaaaaggt gctttattcg ctgtggggag taaaacggta tccgatgccg cgcaccgtct
    13381 taagatagac cgggttggca ggttcatctt cgatatagcg gcgcagccgg acgacaaagt
    13441 tgtctatggc gcgcgtgtct gtgtcctcgc gcacatgcca cacctgttcg agaagctcct
    13501 tgcgggagac aatgcggccg gggttctgga caagatggcg aagaaactcg gcttccatta
    13561 cagtaagatg gatc
//
LOCUS       DASRBV010000312         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_629970, whole genome shotgun sequence.
ACCESSION   DASRBV010000312 DASRBV010000000
VERSION     DASRBV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629970"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFA02_11525"
     CDS             <1..541
                     /locus_tag="VFA02_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZU10521.1"
                     /translation="GVVAVGSGVVSGSIALIGFGVDSFIETSSGGILLWRLRAERRGR
                     NARHAERRALKMVGVSFLLLAAYVTFDAIKSLVSGDTPERSLAGITIAALSLIVMPWL
                     AHAKRKTAGNLGSAALRADSRQTSICAYLSAILLGGLLVNALLGWWWADPVAALGMIP
                     TIVKEGREALHGRPCSDCR"
     gene            755..2227
                     /locus_tag="VFA02_11530"
     CDS             755..2227
                     /locus_tag="VFA02_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZU10522.1"
                     /translation="MDELGQFAKRAGPAVIRMAGAVAGTAGITFLCFRLIQVNATTAG
                     FCYLIEVLIIATAWGLAEAVLTSLVATLCFNYFFFPPVGTLTISDPHNWVALFAFLVT
                     SLIAGQLSAHARQQTREAIDRQKELERLYALSRAILQSSPEHAPGRQIAGQIVKAFQL
                     PGVALADHACGEICVAGTGHLGDLERVLRTGPDNFIPADPGTPIIVSPVRLGDEEIGT
                     LALYRSHLSEAALQSISNLVAIGLDRARAQHTMARAEAARQSQELKSTLMDALAHEFK
                     TPLTSIKAAATALLAQTVPKAPADRELLAIIDDEAGHLSRLVTEAIQMARIEGGKLQL
                     NKGLFVPATLISAALHDMKHRTDGRRVQVNVPDDLPPVFVDAGLIQLSLLQIIDNALK
                     FSYPAGALVITACAKEGRLTIAIADEGPGIPERDISKVFEKFYRCPGHDRVAGTGIGL
                     TVARDILRAHGGDIMVQNIPGKGCEFLLTLAVAPPDEDPV"
BASE COUNT          439 a          681 c          687 g          476 t
ORIGIN      
        1 aggtgttgtc gcagtaggta gcggtgtcgt gagcggaagc attgctctga tcggcttcgg
       61 cgttgactct ttcattgaaa catcatcggg cggcatcttg ctttggcgct tgcgcgccga
      121 gcgtcgcggg cgtaacgcgc ggcacgccga gcgccgcgct ttgaagatgg tcggcgtcag
      181 ctttctactc ctggccgcgt atgtcacctt tgacgcgatc aaatcgctgg tcagcggtga
      241 caccccggag cggagcctgg cgggaattac catcgcagcg ctgtcactga tcgtgatgcc
      301 atggcttgct cacgcaaagc gaaaaaccgc cggaaacctc ggcagcgcag cgttgcgtgc
      361 cgattcgcgt cagacatcga tttgcgcata tctctccgcc atcttactcg gcggcctgct
      421 cgtgaatgcc ctgctcggct ggtggtgggc cgacccggtc gccgccctgg ggatgatccc
      481 aaccattgtg aaggagggcc gagaagccct tcatggccgc ccgtgctccg attgccgctg
      541 atttttggtt ccaacctgcc ccttccctgt ccggttgaga acgaatgcgg tgattcttcg
      601 ctccgttcag gatgacggag gcggaccgcc aagggacaca ccgccgccag gaaaatcgcc
      661 cggccgctcg cagtggggcg tgctatagga tgaaaagtca gtcaaagtga actattgtgt
      721 catttggaga cgagtttggc ggaggtctga cctattggat gagcttggac agttcgcgaa
      781 gcgcgcgggt cccgcggtga ttcgtatggc gggggcggtt gcggggacgg ctggcattac
      841 gttcctctgt tttcggctga tccaggtaaa cgctacgacg gcaggtttct gttatcttat
      901 cgaagtcctg attattgcga cggcttgggg tcttgccgag gccgtcctca cctcgctcgt
      961 agcaacgctc tgcttcaact atttcttttt cccgccggtc gggaccctca caatctccga
     1021 cccccataac tgggtggcct tatttgcgtt tcttgtgacc tcactgatcg cggggcagct
     1081 ttcggcgcac gccaggcagc aaactcgcga ggcaatcgac cgacagaagg aactggaaag
     1141 actatacgct cttagccggg ccattttgca gagcagcccg gagcacgctc ccggcagaca
     1201 gatcgccggg cagatcgtca aggcattcca actcccagga gtcgccctcg cagaccacgc
     1261 ttgcggagag atttgcgtcg cggggacagg acatctgggt gatcttgaac gggtccttcg
     1321 cacaggaccg gacaacttca ttccagcaga cccgggaaca ccgattatcg tcagccctgt
     1381 tcgattgggc gacgaggaaa tcgggaccct ggccttatat aggtcccacc tttcggaggc
     1441 tgcgttacag tcgatttcaa atctggtagc catcgggctc gaccgcgcca gagcacagca
     1501 tacaatggcg cgggcggaag ctgcgcggca gagccaggaa ctcaaatcca ctttgatgga
     1561 cgctctggcc catgagttca agacccctct gacatcgatc aaggccgccg ccacggcgct
     1621 gctggcccaa accgtgccca aggcccccgc ggaccgcgag ttgctcgcca tcattgacga
     1681 tgaggcagga catctatcgc gcctcgtgac cgaggccatc cagatggcac gcatcgaagg
     1741 cggcaagctt caattgaaca aagggctgtt tgtcccggcc actttgattt ctgcggcgct
     1801 tcacgacatg aaacaccgca cagatggacg gcgagtgcag gtgaacgttc cggacgatct
     1861 gcctccggtg tttgtggacg ccggcctgat tcagctctcc ctgctgcaga tcatcgataa
     1921 cgcgctcaag ttctcttacc ctgccggcgc gctggtaatt acggcctgtg ccaaggaggg
     1981 ccgtctgaca attgccattg cagatgaagg cccgggcatt cccgagcgcg acattagcaa
     2041 agtattcgaa aagttttacc gctgcccagg ccatgaccgc gtggcaggaa ccgggattgg
     2101 cctgacggtg gcccgcgaca tccttcgcgc ccacgggggc gacatcatgg tgcaaaatat
     2161 ccctgggaaa ggctgcgagt tcttgctgac gctcgctgtc gcgccgccag acgaggatcc
     2221 tgtatgagcg cagggcgcat cctcatcgtg gatgacgatc cacatatccg gcgcgtaatg
     2281 cga
//
LOCUS       DASRBV010000313         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_275264, whole genome shotgun sequence.
ACCESSION   DASRBV010000313 DASRBV010000000
VERSION     DASRBV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275264"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFA02_11535"
     CDS             complement(<1..581)
                     /locus_tag="VFA02_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10523.1"
                     /translation="MRNQGSMNAQWIPFTGRWAGNSFRCFVVFSGLFLLLPFYAVAQS
                     DVVGNPVALVRRASQNELAASNGNHPFRYQLHKIDDGKITTKEIVETKDGDVARLIAV
                     NDRPLDPDAEKNEIARLNNLAAHPELQAHRRQKEQEDSNRANKMIRLLPDAFVYKYEG
                     MVEGPNGPCYKLSFQPNPNFNPPDHESQVFAGMA"
     gene            759..1160
                     /locus_tag="VFA02_11540"
     CDS             759..1160
                     /locus_tag="VFA02_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZU10524.1"
                     /translation="MAGAGEGTVAAIGKSVQIRGEVKGNEDLLVDGVVEGTITLSDSR
                     LTIGANARVRANVSARDVVVNGTLDGNIQATGRVELRAGCRMNGDLQAQRLSIEENAV
                     YCGKVDLLQGTAATASGAQASTAGALFGASQ"
     gene            1288..1947
                     /locus_tag="VFA02_11545"
     CDS             1288..1947
                     /locus_tag="VFA02_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZU10525.1"
                     /translation="MSVLRTLFGRGQAEARHDKPTVLTGPRVPRHSSGWVALQKYLKS
                     EQALRVLDIGPTSPSNINYLTSLGHSVWMADLVHEALKGGWEIPAEEGGEAGFNVEGF
                     LEQNMDFGGREFDVVLLWTTLDYIPEPLVNPLVDKLYHSLHADGRVLALFHTKKTGPE
                     TAFCRYHLTESEMIEMQESEPFPIQRVYTNRNIEKLFSAYGNYRFFLAKDNLYEVMIT
                     R"
     gene            complement(2063..2482)
                     /locus_tag="VFA02_11550"
     CDS             complement(2063..2482)
                     /locus_tag="VFA02_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10526.1"
                     /translation="MFTPEVFIFIFLSVGAISMFGFMAVASWAGARQQERESYYKNDM
                     LKKLADSSSSGAAATLEYLREQERIALARRAQKKHEGYIVGGMINLAVGVALMVFLHA
                     IVREQPVFYVGLVPGLIGVALIAYALLFLPRSSAGLR"
     gene            2640..3203
                     /locus_tag="VFA02_11555"
     CDS             2640..3203
                     /locus_tag="VFA02_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU10527.1"
                     /translation="MSATEDLADVERVLAGDTASFEGIVRRWQGPLVNMAFRFCNDRG
                     RAEEMAQEAFLRAFRGLASWRRDAAFSTWLFALAANVYRSELKRIPMQSVSLDDIAEL
                     MDKRPHFSALEEADRSQIVRQAVLGLPEKYRDAVILYYFHEMDVPAAAQSLGLPEGTV
                     KARLFRGREILRSKLPQLLGAAALREA"
     gene            3200..3568
                     /locus_tag="VFA02_11560"
     CDS             3200..3568
                     /locus_tag="VFA02_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10528.1"
                     /translation="MMNSDFDEIVSQQDEIVPSSGFVASVMEAVQQETAAPAPIPFPW
                     KRALPIFGAMLAGLVMLVAGIVELFRAPEAFVSAKTAAFSIGSVLSAADKSHTGWVAL
                     ALGLAAFSIFFSLRFISGEK"
     gene            3595..>5561
                     /locus_tag="VFA02_11565"
     CDS             3595..>5561
                     /locus_tag="VFA02_11565"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HZU10529.1"
                     /translation="MIRPVVLAVLCFFSIRMFAQEQKPLNPALVAAKWHAAWIACPDA
                     PQKDEGVYFFRKEITLSAKPEHFWVHVSADNRFLLHVNGQYAGEGPARGDLFHWRFET
                     MDLAPMLHAGKNVIAATVWNFGTRAPVAQMSNRTGFMLQGDTSAEEIANTDRSWLVKQ
                     DKGRGLAAGNGVRGYYAAGPAERVDGRLTTWDWDAERLQDASQWHNAVVIGRAATREA
                     QDADNNWELVQDSLPPMEHGLVAAGKPVRVEGLNAMPEFPEQPLEVPANTHATLLLDN
                     SVLETAYPELTISGGRDAEVRLTYAEALYDAHGQKGNRNEIAGRHIEGVSDEFISDGG
                     EHRSYQPLWWRTWRYLQIDVTTKDQPLRLENLRGWSNAYPFEEKASIDGDIPDLKKLW
                     ETGWRTARLCAHETYMDAPYWEQLQYVGDTRIQALISYADTGDSRLARQAVTNIEASL
                     IPEGLTQSRYPSRLPQFIPTFSLLWVDMLHDYWMYVDDEALVREVLPRTRGVIDWYAA
                     HLRPDGLMGHVRWWEFGDWTSGYRDGVPPQEADGGSTFLTLQFIEALQDAYDMELHLG
                     SSERAASYQAMLKRASDALNRLCWDGQHGVYADTPAKKSYSQQANVLAVWQDVAPRDE
                     QQKILERVLASRENQSVQLDGSTVPPMSAMS"
BASE COUNT         1199 a         1445 c         1708 g         1209 t
ORIGIN      
        1 gccattccgg caaatacctg agattcgtgg tcgggcggat taaaattcgg gttcggctga
       61 aaactcagct tatagcacgg gccattcggg ccctcgacca tgccttcgta tttataaaca
      121 aaggcgtcgg gcagcagacg aatcatcttg ttggcgcggt tgctgtcttc ctgctctttc
      181 tgacggcgat gcgcctgtag ctccgggtgg gccgccagat tgttcaggcg cgcgatctcg
      241 tttttttcgg catcgggatc gagcggcctg tcattgaccg caatcaggcg cgcgacatcg
      301 ccgtctttgg tttcgacgat ttccttggtc gtgatttttc cgtcgtcaat tttgtgaagc
      361 tgatagcgga agggatggtt cccattgctg gcggccagct cgttctgtga ggccctgcga
      421 accagcgcca ccgggttacc gacgacatcc gattgtgcaa cggcatagaa gggcaacaga
      481 agaaacaggc cggagaaaac aacaaaacac ctgaaagaat ttcccgccca tcttccagtg
      541 aaaggtatcc attgagcgtt catactgcct tggttacgca tctatgttgt cttcccgtta
      601 gacgcgcccg ggagcagcgg agttttcccg aaaagggctt catgattgat tttagacggg
      661 tttgtggcgc agggaacagg tcttttcttg cggcattcat ttgggttgcg tatattcgtt
      721 gcaacgcacc agatacgatt tccgggggag aaatttcgat ggcaggcgct ggcgaaggca
      781 cggtagcagc aattggcaag tcggtacaga ttcgcggaga agtaaaggga aatgaagacc
      841 tccttgtaga tggcgtggta gagggcacca ttactctcag tgacagcagg ctgaccatcg
      901 gcgcgaatgc acgtgtgcgg gccaatgtgt ccgcgcgcga cgtagtggtg aacggaacgc
      961 tggacggaaa catccaggcc accgggcgcg tagagctgcg cgcgggatgc cgcatgaacg
     1021 gcgacctgca ggcgcagcgt ctttccattg aagaaaatgc cgtctactgc gggaaagtag
     1081 acctgctcca gggtacagca gctacagcgt ctggcgcaca ggcttctacc gcaggcgcac
     1141 tcttcggagc gtctcaataa agaacaatat tgctggcgat agaattgcta taggaatgcc
     1201 ggcgagcctt tagagcgtat ttccagcgcc ctttcgatga tgacaagggt ttggaggtcg
     1261 caccgtccgc cggaaatcga atccagaatg agcgtactgc gtacactatt tggccgtgga
     1321 caggctgagg cacggcatga caaacctacg gtgctcaccg gcccacgtgt gccgcggcat
     1381 tccagcggct gggtcgcgct gcagaagtat ctgaagagcg agcaggcttt gcgcgtgctc
     1441 gacattggcc cgacttcgcc ttccaacatt aattacctca ccagcctggg ccacagcgta
     1501 tggatggcag acctggtaca tgaagcgctg aagggtggct gggaaattcc ggcagaagag
     1561 ggtggcgaag ctggttttaa cgtggaaggc ttcctcgaac agaacatgga ctttggcggg
     1621 cgggaatttg atgtagtgct gctgtggacg acgctcgatt atattcccga gccgctggtg
     1681 aatccgctgg tggacaaact ttaccactcg ctgcatgcag acggcagggt gctggcgctc
     1741 tttcatacaa agaagaccgg accggaaacg gccttctgcc gctatcatct gacggaaagc
     1801 gagatgattg aaatgcagga gagcgagccg tttccgatcc agcgcgtata cacaaaccga
     1861 aacatcgaga agctcttttc agcatatggc aattaccgct tctttctcgc caaagacaat
     1921 ctttacgaag tgatgattac gcgctgaccc ttgccgcacg tatgcgatga gcgggccaga
     1981 ggggaagccc cctgaattca caatgctgat tcagcagggc aaatcccggt gtttctgccc
     2041 acgtcatttt tgatgatgtg ttctatcgca aaccggcaga ggaccgcggc aaaaataaaa
     2101 gcgcgtaggc gatgagggcc acgccaatca ggccggggac caggccaaca taaaagacag
     2161 gctgttcgcg cacaattgca tgcagaaaga ccataagcgc cacgcctacg gccagattga
     2221 tcattccacc tacgatatag ccctcgtgct tcttctgcgc gcgcctggcc agggcaatcc
     2281 gctcctgttc tctgagatat tccagggttg ccgcagcgcc gctggaactg gaatcggcca
     2341 gcttcttcag catgtcattt ttgtaatagc tctctctttc ctgctgccgc gcgcccgccc
     2401 aggatgcgac ggccataaaa ccgaacattg atatggcccc gacactcaga aaaataaaga
     2461 tgaaaacttc cggtgtaaac attagcgcat ctccttttcg tccggtaaga cattcgtttc
     2521 acaggagcgg tttcattttt tccgtgaagc atgggctgca tccttcgttt tcatgccaaa
     2581 gaaaatgcaa ccgtcatttg cattgtgagg tcatactcgg atgaggaaag ctgccgaagg
     2641 tgagcgcgac ggaagatctg gccgatgtag agcgggtgct cgcaggagat actgcgtcct
     2701 ttgagggcat cgtgcgccgg tggcaggggc ctcttgtgaa tatggccttc cgtttctgca
     2761 acgatcgcgg acgcgcagag gagatggcgc aggaggcctt tctacgcgcc tttcgcggac
     2821 tggcaagctg gcgcagagac gcggcattct ccacctggct ttttgcgctg gctgcgaacg
     2881 tctaccggtc ggagctgaaa cgcattccca tgcaatctgt ctcactggat gatattgctg
     2941 agctgatgga caagcggccc cacttttcgg cgctggaaga agcagaccgc agccagattg
     3001 tgcgccaggc tgttttgggg cttccggaaa agtaccgcga tgccgtcatt ctgtactatt
     3061 tccatgagat ggacgtgcct gccgcagcac agagcctggg actgccggaa ggcacggtga
     3121 aggcgcggct ctttcgcgga cgcgagattt tacgcagtaa attgccgcaa ctgctgggcg
     3181 ctgcggcact gagggaggca tgatgaacag cgatttcgat gaaattgttt cccaacagga
     3241 cgagatcgtt ccgtcgtccg ggttcgttgc ctctgtgatg gaagcagtgc aacaggagac
     3301 agccgcgcct gcgcctattc cctttccatg gaaacgcgct ctcccgattt ttggggcaat
     3361 gctggctggg ctggtgatgc tagttgccgg gattgtcgaa ttgttccgcg caccagaggc
     3421 gtttgtttcc gcgaagacag cggctttcag catcgggtct gtgctttcgg cggcggacaa
     3481 gagccatacc ggctgggttg cactggcgct tgggttggcg gccttctcta tttttttctc
     3541 tctgcgattt atttcagggg agaaataagc tgccgttctg atactctggg ccacatgatt
     3601 cgcccggtcg tattggctgt gctttgcttt ttttccattc ggatgtttgc gcaggagcaa
     3661 aagccgttga atcctgctct ggtggctgcg aagtggcatg cggcatggat tgcctgcccg
     3721 gatgctccgc aaaaagacga aggcgtctac ttcttccgta aagaaataac gttgtctgcg
     3781 aaacctgaac acttctgggt acatgtgagc gccgacaacc gctttctgct gcatgtgaac
     3841 gggcaatatg caggggaagg ccctgcgcgc ggcgatttgt tccactggcg atttgagacg
     3901 atggatcttg ccccgatgct tcacgccgga aagaacgtga ttgcggcgac agtgtggaac
     3961 tttggcacgc gcgctccggt cgcgcagatg agcaaccgca ccggattcat gctgcagggc
     4021 gacacttccg cagaagaaat tgcgaatact gacagatcat ggttggtgaa gcaggacaaa
     4081 gggcgtggcc tggctgcagg caatggagtg agaggctact acgcggccgg ccccgcagag
     4141 cgcgtagatg gccgcctgac aacctgggac tgggacgctg aacgactgca agatgcgagc
     4201 cagtggcaca atgctgttgt catcgggcgc gctgccacgc gcgaagcgca ggacgcagac
     4261 aataactggg agttggtcca agattcgctt ccgccaatgg agcacggtct ggttgcagcc
     4321 ggaaaaccgg tgcgcgtgga gggcctgaat gcaatgccgg aatttcctga acagccactg
     4381 gaagttccgg caaacacaca tgccacgctg ctgctggaca actctgtgct ggagactgct
     4441 tatcctgaat tgaccatcag cggcgggcgg gatgcagagg tccgtctgac atacgcagaa
     4501 gcgctttatg atgcgcacgg acagaaaggc aatcgcaacg aaattgcagg ccggcacatc
     4561 gaaggcgttt ccgatgagtt catcagcgat ggcggtgagc accgctcgta tcagccgctg
     4621 tggtggcgca cgtggcgcta tctgcaaatc gatgtgacga caaaagacca gccgctgcgg
     4681 ctggaaaatc ttcgcggttg gtccaatgcg tatccgtttg aggagaaggc cagtatagat
     4741 ggcgatatcc ctgatttgaa gaagctatgg gaaacgggct ggcgcacggc tcggctgtgc
     4801 gcgcatgaga catatatgga tgctccttac tgggaacagt tgcagtatgt gggcgacacg
     4861 cgcattcagg cgctgatttc ttatgctgac acgggggaca gcaggcttgc gcggcaggcg
     4921 gtgacgaata tcgaggcctc gctcattccc gaagggctga cgcagagccg ctatccatca
     4981 cggttgccgc agttcatccc gacattctcg ctgctgtggg tggacatgct gcatgattac
     5041 tggatgtatg tggatgatga agcgctggtg cgcgaagtgc tgccacgcac gcgcggggtg
     5101 attgactggt acgccgcgca tctgcgtcct gacgggctga tgggccacgt gcggtggtgg
     5161 gagttcggcg actggaccag tggctaccgt gacggtgtac ctccgcagga ggcggacggc
     5221 gggtcaacat ttctgacgct gcaatttatt gaggcgctgc aggatgctta cgacatggaa
     5281 ttgcacctgg gcagcagcga gcgcgcggcg agttatcagg cgatgctcaa gagggcttcc
     5341 gatgcgctga accgcctgtg ctgggacggg cagcatgggg tgtacgcgga tactccggcg
     5401 aagaagagct acagccagca ggcgaatgtt cttgctgtgt ggcaggacgt ggccccacgc
     5461 gatgagcagc agaagatcct ggaacgcgtg ctggcttccc gggaaaacca gagcgtgcag
     5521 ctcgatggaa gcacggttcc gccgatgtcg gcgatgagtt a
//
LOCUS       DASRBV010000314         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_691477, whole genome shotgun sequence.
ACCESSION   DASRBV010000314 DASRBV010000000
VERSION     DASRBV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691477"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFA02_11570"
     CDS             <1..523
                     /locus_tag="VFA02_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HZU10530.1"
                     /translation="AYTPGVMMAWVTDSPNWVNRRTTSLEYRFLSSMQGSLGVGANLN
                     NWTEEDFAAAKYLIEEYKQVRETVQHGALYRLILPADGSEYSVTESVAQDGKQAAVFA
                     FLHSSQMGYPYPRLFLRGLERNAQYRLHWITERPGGQLPESASGSYWMDHGVDLNLRG
                     DFQATAFRLDRVQ"
     gene            complement(718..2424)
                     /locus_tag="VFA02_11575"
     CDS             complement(718..2424)
                     /locus_tag="VFA02_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU10531.1"
                     /translation="MKYLLSAAVTGFFSAVVCLAQSADPNMVPGKFVEVTDQVGVHFQ
                     HVAPHTSKKYLIETMGSGVALFDYDNDGRLDIFLVNGAPFGDPTPKGTIPQKSGPQDW
                     NRLYHQKPDGTFEDVTEKAGLQGVGYGMGVAVGDYDNDGYEDLYVTAYGGNKLYHNNG
                     NGTFTDVTEKAGVGGSGWSTSAAWVDLDNDGLLDLVVLRYIQWDWDDLWCGEHREGYR
                     GYCHPDVFQPISMLVYHNNGNGTFTEEAHKLGLDKPAKALGIAIADYDHDGHIDIMVA
                     NDSMVEFLFHNKGDGTFEEVGLESEIAVDGEGRTYAGMGVDFSDYNNDGLPDLVITNL
                     ANQKYALYQNSGDDSFSYASYISGVGGMTLLHSGWGIRFMDYDNDGWKDLFIAQGHDL
                     DTIEKTFPQLHYREPMLLARNIGGKKFVDVSAISGDVFTKRWVGRGMAIGDINNDGKL
                     DAVVTENNGPAHVLLNETATDNHWLGLKLIGHKSNRDGIGAVVKIITSAGPQWETVTT
                     ASSYLSSSDPRIHFGLGKDTEAQTVEIRWPSGIVQTLKDVKGDHYVMVDEPVNPETTA
                     KK"
     gene            complement(2424..4196)
                     /locus_tag="VFA02_11580"
     CDS             complement(2424..4196)
                     /locus_tag="VFA02_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZU10532.1"
                     /translation="MGGGNATGGVHAPVHDAQNRPITAGGFVDSGPVVFKDISKESGL
                     ASWHHTMGTPEKQFIIETNGSGVCLVDYDNDGWLDIYLVNGSTYDAMQGKATPPHAAL
                     FHNNRDGTFTDVSEKAGVMNDRWGFGCAVGDFDNDGWPDLYVTNFGKNRLYRNNHDGT
                     FTDVAEKAGVTLGNWSTGPTWGDYDGDGKLDLFVPGYVHYDMSNPPVPGSKAVSYNFC
                     QFRGVSVMCGPRGLQGEHDHLFHNNGDGTFTDVSEKAGVSDPNGYYGFTGIFADVNND
                     GKVDLIVGDDSTPNYLYINKGDGTFEDDSYASGFALNQDGREIAAMGIAVGDYQNNGR
                     LDLLATDFSDDYKVLYHNDGDGNFTDVSYHAGIAQDTISFLGWGDGFFDYDNDGWLDI
                     MMINGHVYPQVDKQDWGTTFAERPLLYHNNKGQKFELVPPVKGTGLADVFPGRGAAFG
                     DLFNDGKIDVVVNVIDGTPVLLRNVNPDKHHWIAFRLIGGAKSPRDAVGAQVYLTANG
                     MKQRQDVISGGSYLSSNDMRPHFGLGDATKVDLVEIRWPDGQVEKITPPNVDRIYTVQ
                     EGKGITGELCTSCEKQPEKEAKAK"
     gene            complement(4384..5499)
                     /locus_tag="VFA02_11585"
     CDS             complement(4384..5499)
                     /locus_tag="VFA02_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZU10533.1"
                     /translation="MLRRSLFLCLLAALPLTGFPLHAQRWPEEKANAWYARQPWLVGS
                     NYIPEDAINQLEMWQAETFDPQEIDKELGWAEAIGMNTMRVFLHDLLWQQDEARFRQR
                     IDTFLTIASRHHIRPVFVLFDSCWDPDPKPGPQHPPIPGVHNSGWVQSPGDKALADKS
                     QYPRLKVYVQGVIGAFGKDDRILAWDLWNEPNNGNNSSYGKAELHNKAALVQALLPQV
                     FAWAREEHPIQPLTSGVWEGDWSSLDKMDPITRIQIEQSDILSFHNYGWPEDFEQHVQ
                     WLEQFHRPILCTEYMARGAGSTFDTILPIAKKYHVAAINWGFVAGKTQTYLPWDSWER
                     PYVLMKPPVWFHDIFYPDGRPYRQREVDLIRELTSER"
     gene            complement(5589..>5914)
                     /locus_tag="VFA02_11590"
     CDS             complement(5589..>5914)
                     /locus_tag="VFA02_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10534.1"
                     /translation="YEHLLREFGTDYATVCEQHLTAEKLRDFFAPCEMRRASFSNAQR
                     LTQEGLEGRVLSSSYMPSMEHPRYHAMRAAIADLFEKYEKGGEVCLECECVVCYGLLG
                     RSASV"
BASE COUNT         1269 a         1741 c         1553 g         1351 t
ORIGIN      
        1 cgcctacacg cccggcgtca tgatggcctg ggtcaccgat tctccgaact gggtgaaccg
       61 ccgcaccact tcgctcgaat accgttttct ctcctcgatg cagggctcgc tcggcgtcgg
      121 cgcaaacctg aacaactgga ctgaggagga cttcgccgca gcaaagtacc tcattgagga
      181 atacaagcag gtccgggaaa cagtgcagca tggggcgctc taccgcctga tcttgcctgc
      241 agacggaagc gagtattcgg tgacggaatc ggttgcccag gatggtaagc aggctgcagt
      301 tttcgccttc ctgcactcca gccagatggg ttatccctat ccgcgcctct tcttgcgcgg
      361 actcgaacgc aacgcgcaat accgcctgca ctggatcacg gagaggcccg gaggtcagct
      421 accggaatct gccagcggat cgtattggat ggaccacggt gtggatttaa atctgcgcgg
      481 cgattttcag gccaccgcat tcaggctgga ccgggtgcaa tagcaagggg acatcctgaa
      541 cccatagcat ttttcatata tgtatgtcgt ataagtagag ttcgtcattc tgacggccga
      601 agggaggaag aatcttaacg attgtgggtt taggcaaaaa tgctataact cacgaccctc
      661 ttttcgccgc aaaagcaaaa ttggttcttc agaataacaa cactcagtcg ttccgcctta
      721 cttcttcgcg gtcgtctcag gattcaccgg ctcatctacc attacataat ggtcgccctt
      781 cacatcttta agcgtctgca cgatgccgct gggccagcga atctctaccg tctgcgcttc
      841 agtatcctta cccagcccga agtgtatgcg aggatcgctc gacgatagat aacttgaagc
      901 cgtcgtgacc gtctcccact gcgggccggc agacgtgatg atcttcacca ctgcgccaat
      961 gccgtcgcgg ttgctcttgt ggccgatcag ctttagcccg agccaatgat tgtctgttgc
     1021 tgtctcgttc aggagaacgt gcgccggccc gttgttttct gttaccacag cgtcgagctt
     1081 gccatcgttg ttgatgtcgc caatggccat accgcgtccg acccagcgct tcgtgaacac
     1141 gtcaccggag atggccgaga catccacaaa cttcttgccg ccgatgttgc gcgccagcag
     1201 cattggctcg cggtagtgca actgcggaaa ggtcttctca atcgtgtcca ggtcgtgccc
     1261 ctgcgcaatg aacaggtctt tccagccgtc gttgtcgtaa tccataaagc ggatgcccca
     1321 tcccgaatgc agcagcgtca tgccacctac gcctgatata tagctggcgt agctgaatga
     1381 atcatctccg ctgttctggt aaagcgcgta cttctggttg gcaaggttcg taataacaag
     1441 gtccggcaac ccatcgttgt tgtaatccga gaagtccacg cccattccgg cgtaagtgcg
     1501 gccttcgccg tctacagcaa tttccgactc caggccaact tcttcgaatg tcccatcacc
     1561 tttgttatgg aagaggaact cgaccatcga atcgttggcg accataatgt caatgtgacc
     1621 gtcgtggtcg tagtcggcga tggcaatgcc gagcgccttc gctggcttat cgagccccag
     1681 tttgtgcgct tcttccgtaa atgtgccgtt accgttgttg tggtagacga gcatggaaat
     1741 cggctggaag acatcgggat ggcagtatcc gcgataaccc tcgcggtgct cgccgcacca
     1801 caggtcgtcc cagtcccact gaatataacg cagcacaacc agatccagca gaccgtcgtt
     1861 gtccaggtct acccatgccg cgctcgtgga ccatccactg ccacccactc ccgctttttc
     1921 tgtcacgtcc gtaaacgtgc cgttgccgtt gttgtggtag agtttgttgc cgccatatgc
     1981 ggtaacgtaa aggtcttcat agccgtcatt gtcgtaatca cccacggcca cgcccattcc
     2041 atagcccacg ccctggagtc cggccttctc tgttacgtct tcaaatgtgc catcgggctt
     2101 ctggtggtag aggcggttcc agtcctgcgg cccgctcttt tgcggaatgg tgcctttggg
     2161 tgtgggatcg ccaaaaggag cgccgttgac gagaaaaata tcgagccgtc cgtcattgtc
     2221 gtagtcaaag agcgcaacac cggaacccat cgtctcaatt aggtacttct tcgatgtgtg
     2281 cggggccacg tgctggaagt gcactccaac ctggtcagtg acttcgacaa atttgccggg
     2341 aaccatgttc gggtcggcag attgcgcaag gcagaccaca gcagaaaaaa atccggtaac
     2401 ggcagcagaa agcagatact tcattacttc gcctttgctt ctttctccgg ctgcttctcg
     2461 catgatgtgc acagctctcc ggtaatgccc ttgccttcct gcacggtgta aatgcggtct
     2521 acgttaggag gtgtgatttt ttccacctgc ccatcgggcc agcgaatctc taccaggtct
     2581 acttttgtcg cgtcgcccag cccaaagtgc ggacgcatgt cattcgatga gagatagctg
     2641 ccgccgctga tcacatcctg ccgctgcttc ataccatttg cggtgagata cacctgcgcg
     2701 cccacggcgt cgcgcggact cttcgcgcct ccaataagcc tgaaggcaat ccagtggtgc
     2761 ttgtcaggat tcacgttgcg cagcagcacc ggagtgccat ctatcacgtt caccacaaca
     2821 tctatcttgc cgtcattgaa caagtctccg aaggccgcgc cccgccccgg gaacacatcg
     2881 gctagtccgg tccccttcac aggaggcacc agctcgaatt tctggccttt gttgttgtga
     2941 tagagcaggg gacgctcggc aaaggtcgtg ccccagtcct gcttgtccac ctgcggatag
     3001 acgtggccgt tgatcatcat tatgtcgagc catccatcgt tgtcgtaatc gaagaagcca
     3061 tcgccccatc ccaggaagga aattgtgtcc tgggcgatac ccgcgtgata gctcacatcg
     3121 gtgaaatttc catcgccgtc attgtgatac agcactttgt aatcatcaga gaaatcggtc
     3181 gccagcagat caagacggcc gttgttctgg tagtctccaa cggcaatgcc catcgcggcg
     3241 atctcgcgcc cgtcctggtt cagcgcaaaa ccggaagcat agctgtcatc ttcaaatgtg
     3301 ccgtcgcctt tgttgatgta caggtaattc ggcgtcgagt catcgccgac aatcaggtcc
     3361 accttgccat cgttgtttac atcggcaaag attccggtga atccgtaata gccgttcgga
     3421 tcgctcacgc ctgccttttc gcttacgtcg gtaaatgtgc cgtcaccgtt gttatgaaag
     3481 aggtggtcgt gctcaccctg aaggccgcgc gggccgcaca tcacgctgac cccgcggaac
     3541 tggcagaaat tatatgaaac ggcctttgat ccaggcaccg gagggttgct catgtcgtaa
     3601 tgcacatatc cgggcacaaa gaggtcaagt ttgccgtcgc cgtcgtagtc gccccacgtc
     3661 ggcccggtag accagttgcc cagtgtgact ccggcttttt ccgcaacatc cgtaaaggtc
     3721 ccatcatggt tattacggta gagtcggttt ttgccgaagt tcgtgacgta gagatcgggc
     3781 cagccatcat tgtcaaaatc accgacggca cagccgaacc cccagcggtc attcattact
     3841 ccggccttct cagagacatc ggtgaatgtg ccatcgcggt tgttatggaa aagcgcagcg
     3901 tgcggcggag tggccttgcc ctgcatcgcg tcataggtcg agccatttac cagataaatg
     3961 tccagccagc catcattgtc ataatccacc agacagacgc ccgatccgtt cgtctcgatg
     4021 atgaactgct tctccggagt acccattgta tggtgccagg acgccagtcc ggactccttc
     4081 gagatgtcct tgaagaccac cgggccagag tccacaaacc cgccagcggt aatgggccgg
     4141 ttctgggcat catgaacagg agcatgcacg ccgcccgtag catttccgcc gcccatgggc
     4201 tgttgcggcg cgggggcatt cggcgcggtc tgggcgcaca gaattcccgg aatgacaaga
     4261 caaaacaagg ccgtaaacac acggccgcct gcatacaacg cattcatctg gatgaaacgg
     4321 ggcacgcgct gaatatagca gattcatatg tagacttgtc tccacctaaa gttgtagatg
     4381 gccctagcgc tcgctcgtca gctctcgaat caaatccact tcgcgctgcc ggtagggcct
     4441 tccatccgga taaaaaatgt cgtggaacca caccggcggt ttcatcaata cataaggacg
     4501 ctcccatgaa tcccagggca ggtaagtctg cgtctttcct gcaacaaagc cccagttgat
     4561 ggccgcaacg tgatattttt tcgcaattgg caaaatcgtg tcgaaggtgc tgcccgcccc
     4621 gcgcgccata tattcggtgc agaggatcgg gcgatgaaat tgctccagcc attgaacgtg
     4681 ctgctcgaag tcctctggcc atccgtagtt gtggaaggaa agaatatcgg actgctcaat
     4741 ctgaatgcgt gtgattgggt ccatcttgtc gagtgaggac cagtctccct cccatactcc
     4801 gctggtcagc ggctggatgg gatgctcctc ccgtgcccag gcaaagacct ggggcaacag
     4861 ggcctggaca agcgcagctt tgttgtgaag ttcggctttc ccataagaac tgttgttgcc
     4921 attgtttggc tcgttccaca ggtcccaggc gagaatgcgg tcgtccttac caaatgcgcc
     4981 aattaccccc tgcacataca ccttcagacg cggatactgc gacttgtccg ccagcgcctt
     5041 gtctcccgga ctctgcaccc atccggaatt gtgtacgccc ggaattggcg gatgctgcgg
     5101 accgggcttc ggatccgggt cccagcatga atcaaacagc acaaaaacag gccggatatg
     5161 gtgccggctg gcaatggtca ggaaagtatc tatgcgttgg cggaagcgcg cctcatcctg
     5221 ctgccagagc aggtcgtgca ggaacacgcg catcgtgttc attccgatgg cttctgccca
     5281 gcccagctcc ttgtctattt cctgcgggtc aaaggtctct gcctgccaca tttcaagctg
     5341 gttgatcgcg tcctccggaa tgtaattgct accaaccagc cacggttgcc gggcatacca
     5401 ggcatttgcc ttttcttccg gccagcgctg cgcgtgtagt gggaacccag tcaagggcaa
     5461 ggctgccagc aggcaaagga agagagaacg tcgtaacaaa actgcctcca gaaaaagaca
     5521 agccgatgat aaatggaagc gcttacatcg tccaatgaaa aatatgtccc cccttccagg
     5581 agcgactcct atacagaagc ggatcgcccc agaagcccat agcagaccac gcactcacat
     5641 tccaggcaga cctcgccgcc tttctcatat ttctcaaaca ggtctgcaat ggccgcgcgc
     5701 atcgcatgat agcgtggatg ttccatggac ggcatataag aggaagaaag cactcgccct
     5761 tccaggcctt cctgcgtgag ccgttgcgca tttgagaagc tggcccgcct catctcacaa
     5821 ggcgcgaaga aatccctgag cttctccgca gtcaggtgct gctcacatac tgttgcataa
     5881 tctgtgccga actcccgcag cagatgttca tatt
//
LOCUS       DASRBV010000315        13314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_735378, whole genome shotgun sequence.
ACCESSION   DASRBV010000315 DASRBV010000000
VERSION     DASRBV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735378"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFA02_11595"
     CDS             <1..1048
                     /locus_tag="VFA02_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZU10535.1"
                     /translation="NRANLGLLPEEKGRFGSFGISTIINVVAGAILILLTMAQVRQEQ
                     HRQYQTTELIFPVEQPKPYVLPAPKIKVIPPPPKIEQPKIVLPKPQPEPPKPAPVKLP
                     TPEMPKLTPAPPKAVTPPPQPKVGLFKSATPTRVANNNTAPTPKTGGFGQPNGVTPNP
                     NATRQPTIAAVGAFGNAPGPSTGAGAARQGSVHGTNFGSGVANGVPGGKDRGTVASAG
                     FSNGVVGGTGAPGSHGTVATGGFNTPVGGGSGERVLKPQEPQSTPIVVVSKPLPQYTQ
                     EARQMRIQGDVTLEVRFTASGRVEVLRVVNGLGHGLDQQAILAAEKIRFKPATKNGVP
                     VDQVSVIHITFQLA"
     gene            1160..2986
                     /locus_tag="VFA02_11600"
     CDS             1160..2986
                     /locus_tag="VFA02_11600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZU10536.1"
                     /translation="MLFRKLWIITAAVTLGALTAQAKTQKYAPANPLTPEQSALVQKA
                     VAREKATVQAIQKNTPVVQTYIQNMRPDPKLYAVPDSDQYMLGRVDFSRAFSDKEYAA
                     RGSSRGFFKGSVGFLSGLTKAFQLSYSPTGFMDMMFLDPTSFDQQHYDFSYVRREFLG
                     SVRTMVFDVHPKPNTGAGRFFGRIWVEDQDGNIVRFNGTYTNRRDVEVARYYHFDSWR
                     LNVQPDVWLPVAIYVEESEGGTGKKHAGFRAQTHFWGYSLKLPQRESEAESIVVENAQ
                     DQSENSQDVGPLGAKRQWVSQAEQNVLDRLVQAGLIAPPSDFDKILEQVTNNIIIGNK
                     LNLPGDIHCRVILTTPLESLAVGNTILLSKGLIDVLPTEEDLAAVISFQLAHIVLGHH
                     IDTRYAFNDRLLFPDEATFQRILMNHTAADNEAAAKKAIELFDGSVYHDKSASVSLFF
                     QQVVSRAKAVPALLSPRLGDPLLKADGTPWMSAFLNRGPKLNLEDMNQQLAALPLGSH
                     LKTDPWDDKVYSLNVKAEPILNPGDKMPLEVTPVYFRLQRYQPPASAATAATQPQPQA
                     QPADGNQAQPADGNQQPQGNTAAPQQQPADNTQPQQPQQNPQ"
     gene            3085..3630
                     /locus_tag="VFA02_11605"
     CDS             3085..3630
                     /locus_tag="VFA02_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU10537.1"
                     /translation="MTFFFTSVIASAQVAPATRGGGLTLSAGAEYSNFEPDWGTTRLQ
                     GVTAFFDLDHLVLNRLGVEGEARWLRLNAPHGETQDNYLLGPRYRFVHLGNFHLYGKF
                     LLGGGWMTYPDKLGSGSYFAYAPGATLDYHLTHRWTVRGDYEYQLWPSAPGTAFTAPF
                     GKKSSGLDPNGFSAGVSYRIF"
     gene            3742..4944
                     /locus_tag="VFA02_11610"
     CDS             3742..4944
                     /locus_tag="VFA02_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZU10538.1"
                     /translation="MSKGFKRAILGISVLLVAIVFIGGFGMSGVRAGTQSDGAYRQMG
                     VYEEVLHKIQSDYVVEPNLNKVTTGALHGLLESLDSDSSYLTPAEYTTYKAHENEGAT
                     AQVGLNVSKRGGYATVVSVMPGSPAEREQIQDGDVIVAIGDQPTRDMSLAMIRLLLEG
                     RPGTQVTFSLVRPGRGKYEPDKITLTRVNMTPPAMGEQEYENSSILYLKPVVLTKERI
                     DEIDSRLHAMEKNGNKKVLLDLRDVAQGDEQQAVRLANAFLKTGTIAMLEGQKVSKQT
                     FSADPGKFITNAPLVVLVNHGTSGPGELVAAAILDNKRGDVVGDRTFGEGSVQKTIEL
                     PDGAALILSVAKYESPSGKKIQDVAVTPNVVVASDLDQYQDPDQKPGPHPDDQLNKAL
                     ELLKQKNA"
     gene            5035..7248
                     /locus_tag="VFA02_11615"
     CDS             5035..7248
                     /locus_tag="VFA02_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZU10539.1"
                     /translation="MNQYTQPSPNAFHPGKGRRKVAGGVAIAVLIVLAVAVGSLSGLM
                     LVYTVNLPQIDDLMRYRPNTITELYDTHGRIFGSFALERRIPVNYNDFSPLLREAVIS
                     IEDKNFESHWGVNIFRVLGAAYHDLRSKTRAQGASTLTMQLARNLFLNNERTFGRKLQ
                     EALLSIQIEHAFTKQQIFTLYANQIYLGHGIYGFEAGAQYYFSKHAKDLTLPEAALLA
                     GLPKGPVEYSPILNPEKAFRRRNMVINAMLEDGKITAAQADVARSAPLGLHLQPPPNS
                     VAPWFVEAVRRELEQKLGPDQVHEAGLKVYSSLDLDLQQAANRAVLSGLANYERQRGW
                     RGHLLNVIAGGVGIEGFKHPDWAVPPQSGDYVHALVTGVLPYQVTARVGDHDLLLVPE
                     DWAWTKFRTADTFLKQGDIIYVHLRQPDGNLLRGTLEQDSGAEGALLAMDNATGDVLA
                     MVGGRDFYMSQFNRATQAERQTGSSFKPYVYTAAVEAGAKPDEKVLDAPTSFGSYTPH
                     NYEGNYLGNITLLKAFSDSRNIPALKLAERVGIRKVIDVAHQFGITANIPAYLPIALG
                     SVEVTLEQQVAAYSVFPNDGIRISPRLVRRVTTADGAPLLEDKPAVSEAASARVARTM
                     MVFLREVTRSGTAAAAASLNHPIGGKTGTTSNFTDAWFIGFSPSTTCGVWVGYDNRES
                     LGEKETGAKVALPIWMDFMRAAIAGKPDEAFPGDIKSPPTPAGRIAASVHAAHSE"
     gene            complement(7302..10001)
                     /gene="alaS"
                     /locus_tag="VFA02_11620"
     CDS             complement(7302..10001)
                     /gene="alaS"
                     /locus_tag="VFA02_11620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZU10540.1"
                     /translation="MQFRSGNEIREMFLRFFETKGHRRVHSSSLVPANDPTLLFTNAG
                     MNQFKDVFLGLEQRDYSRATTSQKCVRAGGKHNDLENVGFTRRHHTFFEMLGNFSFGD
                     YFKKEAIDYAWELVTSPEWFGIPKERLYVTIFKGENGVPRDEEAYNLWLAQGVPADRI
                     HEYGMKDNFWQMGDTGPCGPCSEIFYDMGIEASETGEDKPFGQDDSRYVEIWNLVFMQ
                     FDRNAQGELNLLPKPSIDTGAGLERMAAVLQGKISNFETDLFTPLIRRAAELTGQQVS
                     GSASQRASGPELTGNASLRIIADHARAATFLISDGVLPSNEGRGYVLRKILRRGIRHG
                     RLLGQDKPFMHDMVYAVRDEMQNAYPELKESADRVAKVVEAEERQFARVLEVGSRQLQ
                     AALQEGSLSGETAFHLYETYGLPLDFMMDAARDAGVHFDLEGFEQARAEEQARARASW
                     KGGAKQSASSVFRELPKTIFEGYRQLTSTGCEVLAIIKDGQGVQAANPGDTVEVVLDH
                     TSFYADSGGQIGDHGWLYSDDHNAVVAEVSGCTKPVQGVFAHKAVLRQPLRIGDKVDT
                     VVDGNFRAAVRRNHTGTHLLHAALRQVLGTHVKQAGSLVEPNRLRFDFSHFTSIADEE
                     LQDIEDLINKEVLRNTSVETIEDVPIDVAINEYHAMALFGEKYGERVRVVKIGDFSTE
                     LCGGTHTGATGEIGVVKLLGEGSVSSGVRRVEAVTGFGALNEFRRDFAVAQLASQLAP
                     NVELSPADALKQKLSAQEEELKKLRRELEQMRMKSAASAVSNAADQAIEVKGIKVLAQ
                     RVDSLDRGQMRTLVDNLRQKLGSGVIVLGSAQEEGKVALIVGVTKDLTGKVQAGKLVG
                     QIAKIVGGSGGGRPDMAEAGGKDASQLDAALQSAPKVVEELAG"
     gene            complement(10119..10862)
                     /gene="recO"
                     /locus_tag="VFA02_11625"
     CDS             complement(10119..10862)
                     /gene="recO"
                     /locus_tag="VFA02_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZU10541.1"
                     /translation="MIVHQEEAVVLRTWPVHEADLIVSLFTRGHGKIKGIAKAASKSR
                     RRFGGALEPMTHVLANYVIKPRQELVRLDSFEIVASPLSAPIDYTRAAALAFYSEVLE
                     ECMPDHDPHDAVFRLLLSVLEQTTTASVWMPVTYFALWILRLMGWMPDVLHCTAGGEA
                     FAGRPAYWHAHADGLVCAKHKRLASSALSPESQALAIRIFRHPVSAFATEEWPRERAA
                     DLRRFAIQGLERHLERKLSSAHALMKLGG"
     gene            complement(10877..11326)
                     /locus_tag="VFA02_11630"
     CDS             complement(10877..11326)
                     /locus_tag="VFA02_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZU10542.1"
                     /translation="MLTVTSLNFPDNGEIPKKFTCDGENVSPQLSISSVPMEAKSLAL
                     IVEDPDAPGGTFTHWLLFNLPAGTHDLPEGAHVNEARQGKNDFKKNGYGGPCPPPGDP
                     HHYIFHVYALDRMLDLPEGASREQADSALDGHVLGEGTLTGLYRRRQ"
     gene            complement(11483..12067)
                     /locus_tag="VFA02_11635"
     CDS             complement(11483..12067)
                     /locus_tag="VFA02_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10543.1"
                     /translation="MAIVVVGGNARGVGKTSVIAGVISHLPEYTWTAMKITSHTYGFS
                     PVRPKREPIARGTRPQIIEEHRRDSGTDTSRFLAAGAARAFLVCAPSGTLAQAMPAIR
                     NELDKAENAIIESNSILEYLKPDIYLTILDPAAPDFKASAQHFLERADAVLIKATVRP
                     VPHWRQIEGKPQFAIAPPDYMTDAVVDFVKTRLA"
     gene            complement(12071..13060)
                     /gene="lpxD"
                     /locus_tag="VFA02_11640"
     CDS             complement(12071..13060)
                     /gene="lpxD"
                     /locus_tag="VFA02_11640"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZU10544.1"
                     /translation="MNLRDLAKSIGAELRGDDHADVLRVSSISLAAKDSLVFAENEAA
                     LQEALTSNAAAVLVAEGLAAEARAAKPLLVMKQPRLGFARAARLLQAAERPEGIHPTA
                     ILAPEARLGANVSVGAYAVIEKDAEIGAETSIGAGTVIGAGAKVGEQCRIYPRVVLYP
                     GVTLGNRVAVHAGAVLGADGFGYVRDAETGEYLQFPQQGTLVIEDDVEIGANTTIDRG
                     ALEETRILRGVKLDNLVHVGHNVRIGKNVVIAAQTGVSGSSTIGDDAIVGGQVGIADH
                     VEIGEKVILGAQSGIPSRKKIRGPGIVFWGTPARPIKEYLKELATLARLSREK"
BASE COUNT         2851 a         3996 c         3627 g         2840 t
ORIGIN      
        1 gaaccgggcg aatctgggtc ttctgcctga ggaaaaaggc agatttggat catttggcat
       61 cagcaccatc attaatgtgg tagctggtgc gattctgatt ttgctcacca tggcgcaggt
      121 acgccaggaa caacaccgcc aatatcaaac cactgagctg atttttcctg tagaacaacc
      181 caagccgtac gttctcccgg ccccaaaaat caaggttatt ccacctcctc caaagatcga
      241 acagccgaaa atcgtactgc ccaagccgca gcccgaacct cctaaaccgg ccccggtaaa
      301 gcttcctact ccggagatgc caaagcttac gcctgcgccg ccaaaggcgg tcacgccgcc
      361 tccgcagccg aaggtcggcc ttttcaagag cgccacaccc acgcgcgtag ccaataacaa
      421 cactgcccct acgcctaaaa ccggaggctt cggccagcct aacggagtta ctccgaaccc
      481 gaatgcgaca cgtcagccca ccatcgccgc tgtgggtgcc tttggcaatg ccccaggccc
      541 gtccaccgga gcgggtgcgg cacgccaggg atctgtgcac ggcacgaact ttggttcggg
      601 agtcgccaac ggagttccgg gcggaaagga ccgcggaaca gtcgcctccg ccggattcag
      661 taatggggtt gtcggaggga cgggcgctcc tggaagccat ggcactgtcg ccaccggtgg
      721 attcaatacg ccagtcggcg gcggttctgg cgaacgggtc ctgaagccac aggaaccgca
      781 atccacgcca atcgtagtcg tctccaagcc gttgccgcag tacacgcagg aagccagaca
      841 gatgcgcatc cagggcgacg ttacgcttga agtcagattt acagcatctg gccgcgtgga
      901 agtgttgcgg gtggtcaatg ggttaggcca tggtctggac cagcaggcaa tcctggctgc
      961 tgaaaagatc cggtttaaac ctgccacaaa gaatggcgtt cccgtagatc aggtcagcgt
     1021 gatccatatc acattccaat tggcatagaa tcctgagatg gtttgtgaaa agattgggag
     1081 ggtgtcgctg gagtgcgacg gcaatagtag aaaagtctta ggaataacaa agatctatat
     1141 agatccagga gctaagaata tgctttttcg caagctgtgg ataattaccg cagcagtcac
     1201 gctgggagcg ctcactgcgc aggcaaaaac acaaaaatat gcgcctgcca atcctctcac
     1261 tcctgagcag tcagcgctgg tccagaaggc agtagccagg gaaaaggcca cggtccaggc
     1321 cattcagaaa aatacccccg tggtgcagac ctatatccag aatatgcgtc cggacccaaa
     1381 gctctatgca gtcccggact ccgaccagta catgctggga cgtgtggatt tttccagggc
     1441 attcagtgac aaggagtatg ccgccagagg cagttcccgg ggcttcttta aaggctcggt
     1501 cgggttcctt tcgggtttga ccaaggcctt tcaattgagc tattcgccta ccggcttcat
     1561 ggacatgatg ttcctggatc cgaccagctt cgaccagcaa cactatgact tctcctacgt
     1621 ccgccgggag tttctgggca gtgtccgcac gatggtcttc gacgtccacc ccaaaccgaa
     1681 cactggcgca ggccggttct tcgggcgcat ctgggttgag gaccaggacg gcaatatcgt
     1741 tcgctttaac ggaacgtata ccaatcgccg cgatgtcgaa gttgcccgct attaccattt
     1801 cgacagctgg cggctcaacg tccagcctga cgtctggctg cccgtagcaa tttatgtcga
     1861 agaatccgag ggagggacag gaaaaaagca tgccgggttc cgcgctcaga cccatttctg
     1921 gggctactct ctcaagctgc cgcagcggga atctgaagcc gaatccatcg tggtcgaaaa
     1981 cgcccaggac cagagtgaaa attctcagga cgtcggcccg ctcggagcaa aacgtcagtg
     2041 ggtctcgcag gcagagcaga acgtgctcga ccgcctcgtc caggccgggc tgattgctcc
     2101 tcccagcgac tttgacaaga tactggagca ggtcacgaac aacatcatta tcggcaacaa
     2161 actcaacctt cccggcgaca tccattgccg cgtgattctg actacgccgc tcgaatcact
     2221 ggccgtcggc aacacgatcc tgctcagcaa aggactgatt gacgtcctgc cgacagaaga
     2281 agaccttgct gccgtcatca gcttccagct tgctcacatc gtgctcggtc accacattga
     2341 tacgcgctat gcctttaatg atcgcctgct ctttcctgac gaggcgacct tccagcgcat
     2401 ccttatgaac cacaccgctg cggacaatga agcggcagcc aaaaaggcca ttgagctgtt
     2461 tgacggttcc gtatatcacg acaagtctgc cagcgtcagc ctcttcttcc agcaggtggt
     2521 gtccagggcc aaggccgtgc ccgccctgct gagtcctcgt cttggtgacc cgctgctgaa
     2581 ggctgacggt actccatgga tgtctgcctt cctgaatcgt ggtccgaaac tgaacctcga
     2641 agacatgaac cagcagctcg ccgcgttgcc gctggggagc catctgaaga ctgacccctg
     2701 ggacgacaag gtctactctc tgaatgtaaa ggcagagccg attctgaacc cgggtgataa
     2761 gatgccgctt gaggtaacgc ccgtatactt ccggctacag cggtatcaac cgccggcatc
     2821 tgcggccact gcggccacac agccgcagcc acaggcccag cctgctgatg gcaatcaggc
     2881 acagccagca gacggaaacc agcagccgca aggtaacacc gctgctccgc aacagcagcc
     2941 ggcggacaac actcagcccc agcaaccaca gcaaaacccg caataaggga ctgaaatgaa
     3001 gaacaagaac tgctgtactt tatttttctg ctccaagtca gatcactttt tgggagattg
     3061 tgcgtggtca aaaaaatatt tgttttgaca ttctttttca cttcagtcat tgcttccgcc
     3121 caggtggccc ctgccactcg cggaggcgga ctcaccttgt cggcaggcgc cgaatactcc
     3181 aacttcgagc ctgactgggg taccacccgg ctgcagggtg tgacggcctt ctttgatttg
     3241 gaccaccttg tgctgaaccg gctgggtgtg gaaggtgagg cccgctggct gcgcctgaac
     3301 gcgccgcatg gcgagaccca ggacaattac ctgctcgggc cgcgttaccg gtttgttcat
     3361 ctcggcaatt tccacttgta tggaaaattc ctgctcggtg gcggctggat gacctatccc
     3421 gacaaacttg gcagcggcag ttattttgct tatgctcccg gagccacact ggactaccat
     3481 ttgacacacc gttggactgt ccgcggcgat tatgaatatc agctctggcc ctctgctcca
     3541 gggactgcct tcactgctcc ttttggaaaa aagagcagcg gactggaccc caatggcttc
     3601 agcgcaggtg tttcctatcg catcttctaa gtaatgagtg gggccgtcgg catggctcgc
     3661 ggccctcctg agcaaaatca aacgtcgaat ccctagctgg agtatcatct ttttagcagc
     3721 tcctgctgtc tgagtgtgtc catgtccaaa ggtttcaagc gcgcaatttt aggaatttct
     3781 gtacttcttg tcgccatcgt ctttatcggt ggatttggga tgagcggcgt gcgcgctggc
     3841 acccagagtg atggcgctta ccgccagatg ggcgtgtacg aagaggtcct gcacaaaatt
     3901 cagagcgatt atgtcgttga accaaacctg aacaaggtca cgacaggcgc gctccatggc
     3961 cttctggagt cactcgactc cgattccagc tatctgactc ccgccgaata caccacctac
     4021 aaggcccatg aaaatgaagg tgccaccgca caggtaggcc tgaatgtctc aaagcgcggc
     4081 ggatacgcga cagtggtttc cgtcatgccc ggaagccccg ccgagcggga acagattcag
     4141 gatggagatg ttatcgtcgc catcggggac cagcctaccc gggacatgtc tctggccatg
     4201 attcggttgc tgctcgaagg cagacccggc acgcaggtca cattctcact ggtgcgcccg
     4261 ggccgcggca agtatgagcc tgacaagatc acgctgaccc gcgtcaacat gaccccgcct
     4321 gcaatgggtg agcaggaata tgagaattcc agcatcctct atctcaagcc ggtagtgctg
     4381 acgaaagagc gtatagatga gatagactcg cgccttcacg ccatggagaa aaacggcaac
     4441 aaaaaggtcc tgcttgatct gcgcgatgtc gcccaggggg acgagcaaca ggcggtccgt
     4501 ctggcaaacg ccttcctgaa gaccgggacc attgccatgc tggaagggca gaaggtcagc
     4561 aaacagacat tttccgctga cccaggcaaa ttcatcacca acgctccgct tgtggttctg
     4621 gtgaatcatg gaacatcagg cccgggagaa ctggtagctg ccgctattct ggacaataaa
     4681 cgtggggacg tggtcggcga ccgcaccttc ggcgaaggca gcgtgcagaa gaccattgaa
     4741 cttccggacg gcgcagcgct gattctttcc gtagcgaaat acgagtcgcc ttcaggcaaa
     4801 aagatccagg acgtagcggt tactccaaac gttgttgttg catctgacct cgaccagtat
     4861 caggaccctg accagaagcc tgggccgcat ccagacgacc agctcaacaa ggcgctggaa
     4921 ctgctgaagc aaaagaacgc ataacgacgt tttcagttac cagtcagtag tcctctgttt
     4981 tcaatttcag tgatgaccgg ggactgggaa caacatcttc tctggcaaaa ctgattgaac
     5041 caatataccc aaccatcgcc caacgccttt catcccggca aagggcggcg caaagttgcc
     5101 ggcggcgtcg ccatcgccgt tctcattgtc ctggctgtcg ccgttggctc cctcagcggg
     5161 ctcatgctgg tctacaccgt caatcttccc cagattgacg acctgatgcg ctaccgtccc
     5221 aacaccatca ccgagcttta cgacacgcat ggacgcatct ttggttcgtt tgccctggag
     5281 cggcgcatcc ccgtcaacta caatgatttc tctccactgc tgcgcgaagc cgtcatctct
     5341 atcgaagaca aaaatttcga gagccattgg ggcgtcaaca tcttccgcgt gctgggggcg
     5401 gcatatcacg acctcaggtc gaaaacccgc gcacagggcg cctctactct caccatgcag
     5461 cttgctcgca atctctttct gaataacgag cgcacctttg gccgcaaact gcaggaggcc
     5521 ttgctttcca ttcagattga gcatgctttt accaagcagc aaatttttac cctttacgcc
     5581 aatcagatat acctcggcca tggtatctat ggctttgagg ctggagcgca gtattacttc
     5641 agcaagcatg ccaaagacct taccctgcct gaagcggcgt tgcttgccgg actgcccaaa
     5701 ggcccggtgg aatactcgcc tattctgaac cccgagaagg cattccgccg tcgcaacatg
     5761 gtcatcaatg ccatgctgga agacggtaaa atcaccgctg cccaggccga tgtggcgcgt
     5821 tcggccccgc tcggtcttca ccttcagccc ccacccaaca gtgtggcccc ctggtttgtg
     5881 gaagcagtcc ggcgcgaatt ggaacagaag cttggccctg accaggtgca tgaagccgga
     5941 ctgaaggtct actcctctct cgatctcgat ttgcagcagg cagccaatcg ggccgtcctc
     6001 agcgggctgg ccaattacga acgccagcgc gggtggcgcg gtcatctgct gaatgtgatc
     6061 gcaggcggtg tcggcatcga aggctttaag catccggact gggccgtgcc tccgcagtct
     6121 ggcgattatg tccatgccct tgtgacaggt gttttgccct accaggtcac ggcgcgcgtg
     6181 ggcgaccacg acctgttgct cgtccctgag gactgggcct ggacgaagtt ccgtaccgct
     6241 gacacatttc tcaaacaggg tgacatcatc tatgtccacc tgcgtcagcc cgatggcaat
     6301 ctgctgcgtg gcacccttga acaggactcc ggggccgaag gcgcgcttct ggccatggac
     6361 aacgccacgg gagatgttct tgccatggtc ggcggacgcg atttctatat gtcacagttc
     6421 aaccgcgcca cgcaggccga gcggcagaca ggttcttcct tcaagcccta tgtgtataca
     6481 gcggcagtgg aggccggcgc aaagccggac gaaaaggtcc tcgacgcgcc aacctctttt
     6541 ggcagctata ccccgcataa ttacgaaggc aattatctcg gcaatattac cttgctgaag
     6601 gcgttttctg attcccgtaa tattccggcg ctgaaactgg ccgagcgtgt aggcattcgc
     6661 aaagtgattg atgtggccca tcagtttggt attacggcaa atattccggc ttatctgccc
     6721 attgctctgg gctcggtcga agtcactctg gagcagcagg tggccgccta ttccgtcttt
     6781 cccaatgatg gaatccgcat ctctccacgt ctggtaaggc gcgtgaccac tgccgacggc
     6841 gctccgcttc tcgaagacaa acctgccgtc agcgaagctg ccagtgcccg cgtcgcccgt
     6901 accatgatgg tctttctccg tgaagttact cgctctggaa cagccgctgc cgctgcctct
     6961 ctcaaccacc ccatcggtgg caaaaccggg accaccagca acttcactga cgcctggttc
     7021 atcggcttct caccatcaac aacctgcggc gtctgggtcg gctatgataa ccgcgagtct
     7081 ctgggcgaaa aggaaacagg ggccaaagtc gccctgccca tctggatgga cttcatgcgc
     7141 gccgccatcg ccggcaagcc cgacgaggct tttcccggcg acataaagtc cccgccaact
     7201 ccggctggac gcattgccgc gtcggtccac gccgcccatt ctgagtagag cctgtgcgta
     7261 cgtcgcccgg gcgatgtgta gagcagaacg gattgattgt attagcctgc caattcctcc
     7321 acaactttcg gagcactttg cagcgccgca tcaagttggc ttgcgtcttt gccgccggct
     7381 tcagccatgt cggggcgtcc gccgccggag ccgccaacga tcttcgcaat ctgcccgacg
     7441 agctttcccg cctgtacttt tccggtgagg tctttcgtca cgccgacaat cagcgcgact
     7501 ttgccttctt cctgcgccga gccgagcacg atcactccgg agccgagttt ctgtcgcaga
     7561 ttatcaacca gtgtgcgcat ctggccgcgg tcgagcgaat ccacacgctg cgcgaggact
     7621 ttgattcctt tgacttcaat ggcctggtcg gctgcgtttg agaccgccga ggccgccgac
     7681 ttcatgcgca tctgttcaag ttcccggcgg agcttcttca gctcttcttc ctgcgcggag
     7741 agcttctgct tcagcgcatc ggccggagac aactccacat tgggcgcaag ttgcgaagcg
     7801 agctgggcca ccgcgaaatc gcggcgaaat tcattcagcg cgccgaatcc ggtgaccgct
     7861 tccacgcggc gcacgccgga ggagacgctg ccttcaccga gcagcttcac cacgccgatt
     7921 tcgcccgtgg ccccggtatg cgtgccgccg caaagctcgg tcgagaagtc gccgatcttc
     7981 accacgcgca cgcgttcgcc gtacttttcg ccgaaaagtg ccatagcgtg atactcgttg
     8041 atggccacgt caatgggcac gtcttcaatg gtctcaacgc tcgtgttccg gagcacctct
     8101 ttgttgatca ggtcttcgat gtcctgcaac tcctcatcgg caatcgaagt gaaatgtgag
     8161 aagtcgaagc gcaggcgatt cggttccacc agcgatccgg cctgcttaac gtgcgtgccc
     8221 agcacctggc gcagcgcagc atgaagcagg tgcgttcccg tatgattgcg gcgcacggcc
     8281 gcgcggaagt ttccgtctac gaccgtgtct accttatcgc caattctgag cggctgccgc
     8341 aaaacggcct tgtgcgcaaa gacaccctgc acgggcttgg tgcatccgct tacttctgca
     8401 acaacggcgt tgtggtcgtc ggaatagagc cagccgtggt cgccgatctg tccgcccgag
     8461 tcggcataga agctggtgtg gtcaagcaca acttcgacag tgtctcccgg attcgccgcc
     8521 tgcacgccct gcccgtcttt aatgatggcc agcacttcgc atccggtcga ggtgagttgg
     8581 cggtagcctt cgaatatcgt cttaggcaat tcgcggaaca cagagctggc cgattgtttt
     8641 gcgccgcctt tccaggacgc gcgcgcgcgg gcctgctctt cagcacgggc ttgctcaaag
     8701 ccttcaaggt caaaatggac gccggcatct ctggccgcgt ccatcatgaa gtcaagcggt
     8761 aggccgtagg tttcgtagag gtggaaggct gtttcgccgg aaagagaacc ctcctgtagg
     8821 gcagcctgca actggcgcga tccaacctcc agcacccgcg cgaactgtcg ctcctcagcc
     8881 tcaaccacct tcgcaacgcg atccgcactt tccttcaact ctggataagc attctgcatc
     8941 tcatcgcgaa cggcatagac catatcgtgc atgaagggct tgtcttgtcc gagcaggcgg
     9001 ccatgacgaa tgcctcgacg caggatcttg cgcagcacgt agccacggcc ttcattggaa
     9061 ggaagcactc catcagaaat cagaaacgtt gccgcgcgag catggtctgc aataatacgc
     9121 agcgaagcat tccccgtaag ctcaggaccg ctggcccgct gactcgctga accgctgact
     9181 tgctgccccg tcaattccgc agcccgccga atcaatggcg taaacaaatc cgtctcgaaa
     9241 ttcgaaattt tcccttgcag cacagcggcc atgcgttcaa gccccgcgcc ggtatcaatc
     9301 gagggcttgg gcagcaggtt cagctcaccc tgcgcgttgc ggtcaaactg catgaagacc
     9361 aggttccata tctcgacgta gcgcgagtcg tcctgtccaa agggcttgtc ttctccggtt
     9421 tcgctggcct cgatgcccat gtcgtagaaa atttccgagc agggaccgca gggaccggtg
     9481 tcgcccatct gccaaaagtt gtctttcatg ccgtattcgt ggatgcggtc agcgggcact
     9541 ccctgggcaa gccagaggtt gtaggcttct tcgtcgcgtg gaacgccgtt ttcccccttg
     9601 aagatggtca cgtaaaggcg ctctttgggg atgccgaacc attccgggga agtcaccagc
     9661 tcccaggcgt agtcgatggc ctctttcttg aagtagtcgc cgaagctgaa gttgcccagc
     9721 atctcgaaaa acgtgtggtg gcggcgggtg aagccgacat tttccaggtc gttgtgcttg
     9781 cccccggcgc ggacacactt ctgcgaagtg gtggcgcggg agtagtcgcg ctgttccagc
     9841 cccagaaaga catctttgaa ctggttcatg ccggcgttgg taaacagcag agtggggtca
     9901 ttggcaggga ccagagaaga cgaatgcacg cggcggtggc ccttcgtctc aaaaaagcga
     9961 agaaacatct ctcgtatttc gttgccggaa cggaattgca tatcttgagt ctatcaactg
    10021 gatgaggcgg gcacttctgt caggtttgtt ccgggccgga cgcaggctgg caccacgggc
    10081 actcctgtgg gcaacccgcc ggacttcatc tcgtgccttt atccacccag cttcatcagt
    10141 gcgtgtgccg aggaaagctt tcgctccaga tgccgctcca gtccctgaat ggcgaagcgt
    10201 cgcagatcgg cggccctctc ccgcggccat tcttccgtcg cgaatgcaga aaccgggtgg
    10261 cgaaagatcc ttatggcgag cgcctgcgac tccggagaca aagcagagct ggccagacgc
    10321 ttgtgtttcg cgcagaccag accatcggca tgtgcgtgcc agtaggccgg acgccccgcg
    10381 aacgcctcgc cgcccgctgt gcagtgaagg acatcgggca tccagcccat cagtcgcagt
    10441 atccagagcg cgaaatatgt gaccggcatc cagacgctgg ccgttgtagt ctgctccagc
    10501 acggaaagca gcaggcgaaa gacagcatcg tgcgggtcgt ggtctggcat gcactcttcc
    10561 agtacctccg agtaaaaagc aagtgctgcg gcccgtgtgt agtctatggg agcagaaagc
    10621 ggtgaggcga cgatctcaaa agaatcgaga cggacaagct cctgccgtgg cttgatgaca
    10681 tagttggcca acacgtgcgt catcggctcc agcgcgccgc caaaacggcg gcggcttttg
    10741 ctggccgcct tcgcaatgcc ttttattttg ccgtgcccgc gcgtaaaaag agaaacgatc
    10801 aggtcggcct catgcacagg ccacgtgcgc agaacaacgg cttcttcctg atgaacaatc
    10861 aatcaagagc agaatactat tgcctgcgcc ggtatagccc ggtcagagtt ccttccccaa
    10921 gcacgtggcc gtccagggcg ctgtcagcct gttcccggct tgcgccttcg ggcaggtcga
    10981 gcatcctgtc cagcgcgtag acatggaaga tgtagtggtg cggatctcca ggaggaggac
    11041 atggcccgcc atagccattt ttcttaaagt catttttgcc ttgccttgcc tcattcacgt
    11101 gggcgccctc gggcaggtcg tgcgtccccg ccggtaagtt aaacagcagc caatgcgtga
    11161 atgttccacc gggagcgtcc gggtcttcca caatgagcgc aaggcttttt gcctccatgg
    11221 gaacggaaga aatgctgagt tgaggagaaa cattctctcc gtcgcaggta aacttttttg
    11281 ggatttcccc gttatcagga aaattcaggc tggttacagt caacatggaa acagctcctg
    11341 cctttgttgg gatgcttctg gtgcggccgc cgtgtttcca tatcgaaagc catatcacgc
    11401 ctgccacaat cacaatcaca aacaaaagaa gccatagacg ggcttgcttg ccgctggaca
    11461 tcagcgcctc tcattctggg tttcaggcaa gccgtgtttt cacaaagtca acaactgcgt
    11521 cggtcatgta gtcgggagga gcgatcgcga attgcggctt tccctcaatc tgtcgccagt
    11581 gcggaacagg tctgacggtt gctttgatca gcacagcgtc ggctcgctcg agaaaatgtt
    11641 gtgctgaggc cttgaagtct ggtgctgccg gatccaggat ggtcaggtat atgtccggct
    11701 ttaagtattc cagaatactg ttcgattcaa tgatagcgtt ctcagctttg tcaagttcat
    11761 tgcgaatggc cggcatggcc tgggcaagcg ttccggaagg tgcgcacacc agaaaggccc
    11821 gtgctgctcc tgccgccaga aaacgggacg tatctgtccc gctgtcgcgg cgatgctctt
    11881 cgatgatctg cggccgggtg ccccgtgcga taggctcgcg tttgggccga acgggcgaaa
    11941 aaccataagt atgggaagta atcttcatgg ccgtccacgt gtattcaggc agatgcgaaa
    12001 tcaccccggc aatcacagac gtcttgccaa caccgcgcgc attccctccg acaacaacga
    12061 tggccacggt ttacttctct cgcgagagcc gcgccagggt tgccagctct ttcagatatt
    12121 ccttgatagg acgggccgga gtcccccaga agacaattcc cggcccacgt atctttttgc
    12181 gggatggaat cccggactgt gcccccagaa tgaccttctc tccgatctct acatgatcgg
    12241 caattccaac ctgaccgcct acaatggcat catcgccgat cgtactgctg ccggaaacac
    12301 cggtctgtgc ggcaatgaca acgtttttgc caatccggac gttgtgcccg acatggacga
    12361 gattatcgag tttcacgcca cgcaaaatac gggtttcctc cagcgcgcca cggtcaatcg
    12421 tggtatttgc gccgatttcc acgtcatctt caatgacaag cgtcccttgt tggggaaatt
    12481 gcagatattc acccgtttca gcatcgcgga cgtatccaaa gccgtcagct cccagcactg
    12541 ctccggcatg gacggcaacg cgattgccca gcgttacgcc cgggtacaaa accacgcgcg
    12601 gataaatccg gcactgctcg cctaccttcg cgccggcgcc tatcaccgtt cctgcgccaa
    12661 tgcttgtttc cgcgccgatc tccgcatctt tctcaatcac tgcatatgcg cccacagaaa
    12721 cgtttgctcc caatcgggct tccggagcaa gaatggccgt gggatgaatc ccttcaggcc
    12781 tctctgcagc ctgaaggaga cgggcagctc gggcgaagcc aagccggggc tgcttcatga
    12841 ccagaagtgg cttcgccgct cttgcttccg ccgccagacc ctcagccact agaacagccg
    12901 cagcgttgga agtgagagcc tcctgcagcg ccgcttcatt ttcggcaaaa acaagagagt
    12961 ctttggctgc caggctgata ctggagacac gaagcacgtc tgcatgatcg tcaccgcgca
    13021 actccgcccc gatagacttt gcgagatctc ttaagttcat agttcgttca tcgttccgct
    13081 caaattgcat tctcccagat cgtgcagttg gaggagtagg gccttgcact ccgcagattc
    13141 cagaatgggg tcagaaccag acatttctgc tgtcaatccc ttaccgaagt aacaaccgaa
    13201 cgaaatactg gcctttcaat cagatacttc ctggctggcc agccgtgact tgcagccgaa
    13261 acctttatac tggaattaag ggggatattt tttcccgttt ctctgttctt tgaa
//
LOCUS       DASRBV010000316         5392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_20637,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000316 DASRBV010000000
VERSION     DASRBV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20637"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFA02_11645"
     CDS             complement(<1..837)
                     /locus_tag="VFA02_11645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU10545.1"
                     /translation="MRRLFFALLLCSVTVFAQATYTQPVDKLPPELRSKMDEAATKVL
                     EQTGVPSASIAIVQKGEIVYTHAYGKARLEPPTPAEPRMRYSIGSISKQFTAAAILFL
                     EQQGKLSVGDPVSKYIPGLTRGDEVTIRMLLSHTSGYQDYWPEDYLMPPMRKPTTAQY
                     ILDTWAKKPLDFDPGTKWQYSNTNYVIAGLIVEKVSGEPLMKFLQEHIFGPLEMKSVY
                     NTDIAKLGDTDAEGYIRYALGPLRPAPKEGAGWMFAAGELAMPAHDLALWDISVMNRS
                     LLA"
     gene            987..1949
                     /locus_tag="VFA02_11650"
     CDS             987..1949
                     /locus_tag="VFA02_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HZU10546.1"
                     /translation="MPVALDFFVKYGYFILFLWVLAEQLGVPVPSAPLLITAGTLTVT
                     HRLNLLLSIFVVVLACLLSDTVWYYMGKRYGGAVVRLLCRLAMESNTCVKKTEGYFSR
                     NGARALLLAKFIPGLSTVAAPIAGQTGMPMRFFFLYDAAGSLLWALAFILGGRFFGDF
                     LKHHPDIFPSVSHAAGVLFVLLLIGFLVYRFVRQRAFIKEMRAARVEPDELKQMLDKN
                     QQVYIVDLRHPLDYLPDPRTIPGAVMLTPDKLVERTGSIPRDRDVVLFCTCPSEATAA
                     KMALTLRKAGIYRVRPLRGGFDEWKRLGYPLQEIPTETVAQPAR"
     gene            1961..2452
                     /locus_tag="VFA02_11655"
     CDS             1961..2452
                     /locus_tag="VFA02_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZU10547.1"
                     /translation="MRKQWTLCLLAAALLCGTGATLLSQENGPVVPRVPPRPANTLVM
                     LPDGSTVHVELAKTEAEREYGLMGRTSLPQGHGMLFIHDRPGEYSYWMYHCKIGLDII
                     WMDADHRIVELSPNTPPCKDKTGNCPSYGGHATSLYVLELPVGSIKAHNLQTGQVVNF
                     NVN"
     gene            complement(2640..4175)
                     /locus_tag="VFA02_11660"
     CDS             complement(2640..4175)
                     /locus_tag="VFA02_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZU10548.1"
                     /translation="MMSKSRIFTFLWTAAVCALYAVHLFHLRADFPNYSPWMDYSKYT
                     DEGWYGSAAVRYILTGSWRVQGDFNPAAAVPMWPVLEGLVFHFTGVSIVAARALTLVV
                     FGANLLLTWALFRVQQQKTWTTLLAVTLLAGNAFLYSFSRLALLEPALIFWTLLAWLL
                     ILRAPEAAGARVRCTFLVAAGLCLCLAILTKTTGIFLLPAAFYLLWHSAEHRVRVFAR
                     YAAVTGAAAGISWSAYYFLLVHPRYLNDYYYLFAVNQMPQPVTVGGWIGAFWYAAHGA
                     LWIDKPLTVLTAVLIVFSVFFLRRVWRNGLFPAALLAIAGYVLFIGYHNNMQPRYYQV
                     IAFPLVFVLCLGLAALWERWRWAGMGALAVVLASLALNLRMVAGYARHPEYTFVNAAN
                     ELTRYIDQHSEDGNRLLLSISGADITLITHLPSICDDFGTYDLPYRIHTYHPGWYAAW
                     NELDPGSVADINTQYSLKEVAHFPAFDDPDRDDLVLYKMVPLPAEKQTYNAAEEVAEN
                     AGK"
     gene            complement(4207..4932)
                     /locus_tag="VFA02_11665"
     CDS             complement(4207..4932)
                     /locus_tag="VFA02_11665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU10549.1"
                     /translation="MNSLQGKAALVTGAAKRIGRALALALARSGANVAITYRGSEKEA
                     QQTIKDLQALGAEALAVRCDVRKPKDVEEAVAAAVRQFGRLDLLVNNAGAFETAKLEQ
                     ISVEQWDAMFETNTRGPFLMAKAAYPHLKASRGRIINIGSLGGLHPWPTHGHYCTSKA
                     ALHMLTETMSKAFAPEISVNCVAPGMIVNGEVSEDYEHFAQKTPMKRNGTQEDVAAAV
                     MFFATGPHFITGQILSVDGGLGL"
     gene            5049..>5392
                     /locus_tag="VFA02_11670"
     CDS             5049..>5392
                     /locus_tag="VFA02_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10550.1"
                     /translation="MLSQLPRIALMALVVWPLMESCSRPVHSNLLQAGAPGLQSDDTP
                     HLRREGNPNAPYLVASAKPQLVKRSDNTGNTAAETCDGKDLSVTEIAAAVNGDYHAVK
                     LAFYNQGLTACK"
BASE COUNT         1205 a         1709 c         1332 g         1146 t
ORIGIN      
        1 agccagcagc gaccgattca tcacgctgat gtcccacagc gccaggtcat gcgccggcat
       61 cgccagctca cccgccgcaa acatccaccc tgccccttcc tttggagcag gccgcagcgg
      121 ccccagcgca taccggatat acccctcggc atcggtatca cccagctttg caatgtctgt
      181 gttgtagact gacttcatct ccaacggccc gaaaatgtgt tcctggagaa acttcatcag
      241 cggctcgccg ctgaccttct ccacaatcag ccccgcaatc acgtaattgg tattggaata
      301 ctgccacttc gtgccaggat caaaatccag cggcttcttc gcccatgtgt ccagaatgta
      361 ctgtgctgtt gtcggcttcc gcatcggagg catcaggtaa tcctcaggcc agtagtcctg
      421 gtatccggaa gtatgcgaaa gcagcatccg tatggtgact tcgtcgccgc gtgtcaaccc
      481 gggtatgtac ttcgacaccg gatcacctac agacagtttg ccctgctgct caagaaacag
      541 aatggccgcc gccgtgaatt gcttcgagat agagccaatc gaataccgca tccgcggctc
      601 agccggagtc ggtggttcca ggcgcgcctt gccgtatgcg tgcgtgtaca caatctcacc
      661 cttctgcaca atggcgatcg aagccgaagg cactcctgtc tgttccagga ccttcgtcgc
      721 cgcctcatcc atcttgctgc gcagctcagg cggcagtttg tcgacaggtt gtgtgtaagt
      781 agcttgagca aaaacagtga cagaacacaa aagcagggca aaaaacagac gtctcatctg
      841 cattctccgg aaaagatgga agaatcagcc tagcacacga aacatctgtt ttatgcttcc
      901 ggattcctcc cctcaaaccc tccccttgcg cccttcgttg ccattcccgc atctaaatgg
      961 catcctgtaa tcctgccggg aaagccatgc cagtcgctct cgattttttt gtgaaatacg
     1021 gctatttcat cctgttcctc tgggtgttgg ccgagcagct tggcgttccc gtccccagcg
     1081 cgcctctttt gatcaccgca ggtacgctta ccgtcactca ccgcctcaac ctgctgcttt
     1141 caattttcgt tgtagttctt gcttgtctcc tgagtgacac cgtctggtac tacatgggca
     1201 agcgctatgg cggagcagta gtccgtctgc tctgccggct cgctatggaa agcaatacct
     1261 gcgtcaagaa gacagaaggc tatttttccc gcaacggcgc gcgcgcgctt ctgctggcga
     1321 agttcattcc cggactcagc accgtggctg ctcccatcgc cggacaaacg ggtatgccca
     1381 tgcgcttttt ctttctgtac gatgccgcag gatcgctgct atgggcactg gccttcatct
     1441 tgggcggccg cttctttggc gatttcctca agcatcatcc tgacatcttc ccgagcgtca
     1501 gtcatgcggc tggtgttctg tttgttctcc tgctcatcgg cttcctcgtc tacaggttcg
     1561 ttcgccagcg cgcctttatt aaggaaatgc gcgcggcccg cgtcgaacca gacgaactca
     1621 agcagatgct cgacaaaaat cagcaggttt atatcgtaga cctgcggcat ccgctcgatt
     1681 acctgccgga cccgcgtacc atccccggcg ctgtcatgct tacaccagac aaactcgtcg
     1741 agcgcaccgg ttccattcct cgcgaccgcg atgtggttct gttctgcacc tgtcccagcg
     1801 aagccactgc cgcaaagatg gccttgaccc tgcgcaaagc tggtatctac cgtgtgcgcc
     1861 cattgcgcgg aggcttcgat gagtggaagc gcctcgggta ccctctgcag gagattccta
     1921 cagaaacggt tgcacagcct gcccgctaaa atgaaaatca atgcgaaaac aatggaccct
     1981 ctgtctgctc gccgccgctc tgctctgcgg taccggagct acccttctct cccaggaaaa
     2041 tgggcctgtg gtccctcgcg tacctccccg ccctgcaaat acgctggtca tgctgcctga
     2101 cggctccacc gttcacgtag aactcgcaaa gaccgaagcc gagcgcgaat acggcttgat
     2161 gggacgtacc agccttcccc agggacacgg aatgctcttc atccacgacc gtccaggcga
     2221 atacagctac tggatgtatc actgcaaaat cggcctcgac atcatctgga tggacgcaga
     2281 tcaccgcatc gtagagcttt ctcccaacac cccgccttgc aaagacaaaa ccggcaactg
     2341 cccctcctat ggaggacacg ccacttctct ctacgttctt gaacttcccg taggctccat
     2401 caaggcacac aacctgcaaa ccggacaagt cgtaaacttc aacgtgaatt agggcactgg
     2461 tgtggtaagt tacaggtccg cagtataaat ctcagcatac ccgtcattct gacggccgaa
     2521 gggaggaaga atcttggcgg tgtgaggccg ggtgccccat gaaatcccct tcctccttcg
     2581 gtacttcata atatgagatg cggtagaaga aagttgctcc ccacccaact acacacgcat
     2641 cactttcctg cattctccgc cacttcttct gcagcgttat acgtctgttt ctcagccgga
     2701 agcgggacca tcttatacag gaccagatcg tctctatcag gatcatcgaa cgccgggaag
     2761 tgcgccactt ctttcagcga atattgcgtg ttgatatcgg ccaccgatcc agggtccagc
     2821 tcattccatg ccgcatacca cccggggtgg tatgtgtgaa tgcggtaagg cagatcatac
     2881 gtgccaaaat catcgcagat agacggcaga tgcgtaatca gcgtgatgtc tgctccgctg
     2941 atggaaagca acaaccggtt gccatcctca gaatgctggt caatgtagcg cgtaagctcg
     3001 ttggccgcat tcacaaacgt gtactccgga tggcgggcat aaccggccac catgcgcaga
     3061 ttcaaagcca ggctcgccag aaccacagcc agagcgccca tgcccgccca tctccaacgc
     3121 tcccacaaag cagccagccc cagacacaaa acaaagacaa gcggaaacgc aatcacctgg
     3181 taatagcgcg gctgcatgtt gttgtggtaa ccaatgaaca gaacatatcc ggctatggcc
     3241 agcagcgccg cgggaaagag cccattccgc cacactctgc gcagaaagaa caccgaaaac
     3301 acaatcaaca ccgcagtcag caccgtcagc ggcttgtcta tccacagggc gccgtgcgct
     3361 gcgtaccaga atgcgccgat ccatcctccc accgttaccg gctgcggcat ctggtttact
     3421 gcaaaaagat agtagtagtc attgagatat ctcggatgga caagcaggaa ataatacgcg
     3481 ctccaggaaa tgcccgcggc ggcgccggtc accgctgcgt atctggcgaa cacccggacg
     3541 cggtgttccg cactatgcca cagcagatag aacgctgccg ggagcagaaa gattcccgtc
     3601 gtcttggtaa gaatcgccag gcacagacac agccctgcgg ccacaaggaa cgtacagcgt
     3661 actctcgcgc ccgcagcctc aggcgcgcgc agaatcagaa gccaggccag caatgtccag
     3721 aagatcagcg ccggctccag caatgccagt cggctgaagg aataaagaaa cgcattgccc
     3781 gcaagcaatg ttaccgccag cagtgtggtc catgttttct gctgctggac acgaaacagc
     3841 gcccacgtca gcagcaggtt cgccccgaat accaccagcg tcagcgcgcg cgccgccaca
     3901 atgctcactc cggtgaagtg gaagaccaat ccttccagca ccggccacat aggtaccgca
     3961 gccgcgggat tgaagtcgcc ctgaacacgc catgatcccg taagaatgta ccggacggcc
     4021 gcgcttccat accagccctc gtccgtgtat ttggagtagt ccatccacgg cgagtagtta
     4081 ggaaaatccg ctctgaggtg aaagaggtgg acagcataaa gcgcgcagac cgcggcggtc
     4141 cataaaaaag taaatatccg ggatttgctc atcattcctt tcaacactct tgataaagcc
     4201 ttgcttctat agtcccagcc cgccatccac gcttagaatc tgcccggtaa taaaatgcgg
     4261 cccggtggca aagaacataa cagcagccgc tacgtcttcc tgcgtgccat tccgcttcat
     4321 aggggtcttc tgcgcaaagt gctcgtaatc ttctgagact tccccattca caatcatccc
     4381 cggcgccacg cagttcacac tgatttccgg cgcaaaggcc tttgacatcg tttccgtcag
     4441 catatgcaac gccgcctttg acgtgcagta atgcccatgc gtgggccacg ggtgcagtcc
     4501 gcccagtgag ccgatattga taatgcgccc acgcgaagcc ttcaggtgcg gatacgccgc
     4561 cttcgccatc agaaatggcc cccgcgtatt ggtctcaaac atcgcgtccc actgctcaac
     4621 cgaaatctgt tccagcttcg ccgtctcaaa ggccccggca ttattcacca gcaggtccag
     4681 tcgcccaaac tgccggaccg ccgctgcaac agcctcttcc acgtctttgg gcttacggac
     4741 atcgcagcgc accgccagcg cctcggcccc cagcgcctgc aaatctttga ttgtctgctg
     4801 ggcttctttc tctgagccac ggtaggtaat ggcaacgttc gcgcctgatc ttgccagcgc
     4861 cagcgccagc gcgcgcccaa tgcgtttggc cgcgcccgtg accagagcgg cctttccctg
     4921 taaactgttc atatgtcctg attttacctg tctggagaga aatgctcttc cggcagtcaa
     4981 tccacgagac tgcaacgtat tcagtgcagc cggcatctta gtaaacgaat tttctctgag
     5041 gcctttccat gctttcccag ttgccacgta ttgctcttat ggccctcgtc gtgtggcctt
     5101 tgatggaaag ctgttcccgc cctgtccaca gcaacctgct tcaggcaggt gctcctggcc
     5161 tccagagtga tgacacaccg cacctgcgcc gggaaggaaa cccgaacgcg ccctatctgg
     5221 tcgcttctgc caaaccgcaa ttggtaaaac gctccgacaa caccggaaat actgccgctg
     5281 aaacatgcga tggcaaagac ctgtccgtaa ccgaaattgc cgcggcggtc aacggcgact
     5341 accatgccgt aaagctggct ttctataatc agggacttac ggcctgcaaa at
//
LOCUS       DASRBV010000317        16587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_228956, whole genome shotgun sequence.
ACCESSION   DASRBV010000317 DASRBV010000000
VERSION     DASRBV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228956"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFA02_11675"
     CDS             <1..619
                     /locus_tag="VFA02_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coatomer subunit epsilon"
                     /protein_id="HZU10551.1"
                     /translation="IAVVVLAGLFFAGLITYNNRSERAASLFGQAMDIYSTPIAQPGQ
                     PAEPGVKTYASAAERAKAAYPLFEEVASKYGWLKEGVNARYLAGMSALDMGQTSTGEN
                     DLKKAADSSDSNVAALAKLALAGIYHQTGRDSQAVALYQQLIAKPTATVPASLAKLQL
                     AQMYESTKPDEAKKLYAEIKDQDKGTAAGQIATQKLQGAVAVPQQ"
     gene            799..1329
                     /locus_tag="VFA02_11680"
     CDS             799..1329
                     /locus_tag="VFA02_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU10552.1"
                     /translation="MRTQEETGEIITALVAEYSTMLYRVAYSVIRNAAEAEDIVQEAF
                     LRVLRHKEKLNEIRDARVWLVRIAWNLALDRKRRAKTRPETEDIDAHARTLTTAERRA
                     DQEVISSQECARILGLIDRLPKKEREALLLSAVEELSTTEIAAICSTTESSIRSRIFR
                     ARKELALLLAKERGGQ"
     gene            1326..1796
                     /locus_tag="VFA02_11685"
     CDS             1326..1796
                     /locus_tag="VFA02_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10553.1"
                     /translation="MKHAFDKKIDAVLHRIAHVQPEPGLEKRIHARLERESARMEKNP
                     AVRLRRFFLAQRLAFAAAATVAGCAVMVVGSVQHSRQKALEGVAPPAGIHLGAPESGV
                     GAASGVRISPQPMVAPENTHARSERKVTNGRATVLKDAHKPTGVAVPGSPEQKP"
     gene            complement(1831..2154)
                     /locus_tag="VFA02_11690"
     CDS             complement(1831..2154)
                     /locus_tag="VFA02_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZU10554.1"
                     /translation="MRWFLDALALLIVAWLVPGFRVLNLPSALVAVIVIGLLNVTIGF
                     LLKLITLPLSILTLGLFLLVINALILRMASAVVPGFQVNSFGAAFIGAAVLAILHMLF
                     EALSS"
     gene            2251..3090
                     /locus_tag="VFA02_11695"
     CDS             2251..3090
                     /locus_tag="VFA02_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10555.1"
                     /translation="MKNFLPTMLCACTATIFAQSAQAPLPDVPTLIRQVEEHQHKLDQ
                     VRENYTYHETAVTHELDKNGKIKKTESEEDEVFFVNTHEVHRKIKKNGKELSPDEQKK
                     EQDRVQKEVEKALKTPPGESTDKHEVSVSRLLQIMRVSNPRREQMDGRSVITFDFVGD
                     PRAKTHGDEAVLKKISGTVWIDEKDREVRRLTARFDDNFHMGFGLFSIGKGSNLTFDQ
                     KLVNNELWLPTGAQIHIVAHAIGVIGWRGDIAVTDDDYQRFHADASQQPGATVVRSSD
                     PQH"
     gene            complement(3146..3604)
                     /locus_tag="VFA02_11700"
     CDS             complement(3146..3604)
                     /locus_tag="VFA02_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10556.1"
                     /translation="MRKLFCTLALTGLLGFGVTAAIAQDASSQQQPETAPPMHGPRHQ
                     MGNPDAQLAHLTKALNLTSDQQTQIKPLLENQQQQIQQVRQDTSLSRQDKMSKVQSIH
                     EDTKGKIEAVLNDQQKQKYEAIQQRMQERHHRGQGGEAQPQSQPQSQPQQ"
     gene            complement(3744..4355)
                     /locus_tag="VFA02_11705"
     CDS             complement(3744..4355)
                     /locus_tag="VFA02_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10557.1"
                     /translation="MLKSGILFRSAFFLLPMIAVSAGSAVTHHPGIAVPLCSHISLSA
                     SYLAEAEPGKGPGFLFVIKNDTNKEIRLAKPVPTSAHWYAHVGEKWLWRASSGTGGSL
                     VDANHEKGEVFAYQPAQPPQHAEYMTVPPHSSQEWTESIKDHPAIAYRPGCAICNYPG
                     EREYRAIFAYAYVPHPQEHAEGLLPCGLRSEPVVMPPLTKNSR"
     gene            complement(4596..8252)
                     /locus_tag="VFA02_11710"
     CDS             complement(4596..8252)
                     /locus_tag="VFA02_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10558.1"
                     /translation="MTQICLWAGILTSGTAPAQTVRGTLSGTITDSSGAVIANATVQA
                     KNEKTGDISTVVSTGAGAYRFPELPLGTYDVTVSAAGFQNSVSTGILVQLQQVTTLNV
                     TLQPGAAAQTVTVNANAPTLQTESSDIGGIVASRQIVELPLALGGVGALRSPEAFVFL
                     LPGTTGPGTANSNNGIFLSKIAGGQNYGNEVLIDGASQQRSENGSSFDEEAPSVEALQ
                     EFKITTALPPAEYGRTTGGIENFVTKSGTNDFHGTAYEIFRNEDLDANTWFNNGNRAY
                     YCSGSNNTRECRKQYDTPEDKKNDYGVTLGGPVWIPKLYNGRERLFFFFSWEQLGYKL
                     GATNISTVPTIAMRNGDFSGPLIYRTDLKPVGTNPCQGNAPVYQGEIFDPLTERTVST
                     PNGPVTCRDQFPGNKIDPSRFSPVAKNLLNYYPLPTNDQQINNFAFTSAIPINNTTYT
                     VRIDANLTEKSRIWGSYSARENNRTSGGNPILPDPVDPNTWKQDFLTHFGRFGWDYTF
                     TPNLLNHLNFGSNRSNSINYAQAIFANKNWSQQLGIANADSKNFPQITNGNVDNLGNP
                     PQNDDNIDNGLRLNESVAWQHGRHSLAFGTDIRWQQYSPINGNSPTINFCGTQTSSSR
                     GIGDGYGFASQMLGLACGGSQSIIPHQSRWTSWYYGLFIQDDFKVTRDLTLNLGLRWD
                     VDMPRHEAKNFTSNFSPTANDPEFNIPGAMVFGTNCKGCNTAWADTWYKDIGPRIGFA
                     YSPSQLEGKTVFRGGAGILYGPLQYSDFGGSMNTGYRANPSFPDNGFDPSFSLDTGYP
                     AFAQPPNLDPGIFNGKPVSGSYIEKQYGRPAMVSEWTLQMQQQLATDLIMTVGYVGNK
                     AQNLHSNIQNINNIDQKYFSLGNHLSEAFAGNSLGIPEPFPGFNQLWNGADVERALRP
                     FPQYDYIDSGCCLQNVGMSSYEAMLVSLERRFHNGLNLQASYTWAKQITNSDSLLPNN
                     GVGVAQVQNVNNLHQEKAISAQDVPHTVVLSWLYDLPFGRGKTWLNHGFASYVVGGWE
                     IGAVQRYMSGQPFSFCCASGIPGFQNQIRFDRVSGSDLKSPAYHRGHINPIGATRADP
                     QTNTLFHLSTTNATQVNADGSVTIIDPHPAFYDQNDARNRGAYGAFSLGNMPRVTGEV
                     RTPAYYNEDFSILKNTPIHENIVFQLKFELLNAFNRHTWSIPDSAPNDLLFGVPGGTL
                     NNPRNVQITGRINF"
     gene            8641..9564
                     /locus_tag="VFA02_11715"
     CDS             8641..9564
                     /locus_tag="VFA02_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU10559.1"
                     /translation="MAVLSGCHEGNYRVAVIPRTTALTIWEAVHAGAEFAARPAGIQV
                     YWNAPTSEDDIQQQAALVDRVVDEHYQGLVLAPDQPLALMSPVRRAIAQGVHTVVIAS
                     PLSIPAQENLAYIVNDDEAAGRIAALRLGQVLSGHGSVAVVGINPESLSDLTILHAFE
                     AALKEHFPDITITDLRTGTHNVTEAQQIADEVLATHSGLGAIFSLSAMASNGAVTALE
                     TRGLAGKVKLIGFEQSVELGNEVRAGTMDSLIAEDTYEMGHRALSMLAQYRSSSLPGQ
                     EIRLQPSLLTRENIDSPLARRLINRDWGQKP"
     gene            9561..11690
                     /locus_tag="VFA02_11720"
     CDS             9561..11690
                     /locus_tag="VFA02_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU10560.1"
                     /translation="MIRTWFFAAVCVLAAATAVFYWRSPWRGLPYQDSFAARTSSEWK
                     PYAGNWSVVRGGIRNDSNERGAKLVTGSPFWRDYSVEADIELLGEGGDAGLMIRASDV
                     ERGVDSYDGYYAGVRTTDQSLVLGRAEHGWIEFPPAKMPGGVKPGRWYHLKLGAFGCS
                     ISASAQEMGTNNRQTVTAHDPNCFRSGKTGLRSMVAGGVWRNVRAERISSAEAALLSS
                     AKSPAQMAVYPTNQGTLPVPENVPAPVAEGPHESGPVTPIQNLRLFPVTHAAHAVVRG
                     TVILTDPLYVQDSSGGVQVDLKTKSLLRVGDEVEVEGDVYPHGLTATLRDAKVHSLGG
                     MAPKPPLSVTADQAATGAYHAMFLEVEGTLQSKAATPDGMTMLELRDGQQVFRALPAS
                     LAVSASFKKLEKNSLLHLRGVCLVNAAYTQNAVPFALLVSSSEDVKVLVGPPWWSPEH
                     LIALALIMLAAGYFVHFLYSRAEQWRLRAVIDERERLAHEIHDTLAQSFAGIGFQLRA
                     IRNRISRSNGPLDHSTLLEELNRASELVRHSHDEARRSITTLRPDATEAGGLIAALEQ
                     IARQMVGRNSVHIETSVEGEARPIPLRVLDCLFRIGQESIANAIQHGHPSRLTIRAVY
                     LPTEITLVIEDDGSGFVPRHDSDGFGLSGMRRRAESIGGELKIETAPGKGTRIVVDAP
                     APPEKHRLLRIAYDEGKHVPNPHSDSR"
     gene            11659..12444
                     /locus_tag="VFA02_11725"
     CDS             11659..12444
                     /locus_tag="VFA02_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU10561.1"
                     /translation="MSRIRILIADDHPVVRAGLASMLATYPDLDVVGSVSDGQQAIEI
                     LKAQPVDILLLDLRMPALNGEQTLRAIRQLDSPPRVIVLTSYESDEDIYGAIRAGAHG
                     YLLKASSEEEMMEAIHAVYGGGRYFPKHIAARFADRVPRANLSRQQQEILDLIAEGTT
                     DTEVAEKLGLSVSEVWERLNTIIETLDALEEARAQGGKVTIADIARKAGVSMATVSRV
                     LHNKGKHTEETRRAVMKVVREYDFQINDKAASLAMMRSSSQQD"
     gene            13578..14216
                     /locus_tag="VFA02_11730"
     CDS             13578..14216
                     /locus_tag="VFA02_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZU10562.1"
                     /translation="MNYSKIAYFFMGRFRKKRLKMFSAAFPPSTYHTVLDIGGTPHIW
                     DMLGDSYEVTLLNNDRREFEAGGGRYQHYTCVVGDGCHLQFPDKSFDLAFSNSVIEHV
                     GNWGEMERFAAEQRRVGKSFYCQTPNKWFPVEPHLGTLFFHWWPRLLRIYFVTRYLTL
                     WGLMNKPDREQVEKSLDNIHLLTRQDLERLFPGAKIVSERFFFLTKSYIVAG"
     gene            14690..15184
                     /locus_tag="VFA02_11735"
     CDS             14690..15184
                     /locus_tag="VFA02_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZU10563.1"
                     /translation="MAITRWDPFRDVLALQNRLNSLFDDFTRGQGEQDALTTAAFVPP
                     VDIYEDDHKVVLKLEVPGIRQDELDIRVENNTLTVRGERKFEKEEKQENFHRIERRYG
                     SFFRAFTVPSTVDTDSVHASYDAGVLKIEMQKKAEAKPKQIKVNVGSSPKPIEAGKAE
                     KTAA"
     gene            15367..>16587
                     /locus_tag="VFA02_11740"
     CDS             15367..>16587
                     /locus_tag="VFA02_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZU10564.1"
                     /translation="MAIRWDKFTVKSQEAVQAASSLATENGNPELLPLHLLAALLEDK
                     EGIIVPVLEKVGVPTSQLLSKAQEAIGRLPKVSGGGGQPGMSAALQKVLEQAFKEADN
                     FKDEYVSTEHLLLALTQQKNDGAQLLLAAFGATHDAILKGLTTVRGNQRVTDQNPEAK
                     YQALEKYAKDLTELARRGKLDPVIGRDEEIRRVVQVLARRTKNNPVLIGEPGVGKTAI
                     VEGLARRIVSGDVPEVLRSKRVIALDLGSMLAGAKYRGEFEDRLKAVLKEIEESSGQI
                     ILFIDELHTLVGAGAAEGAIDASNMLKPALARGELRAIGATTLNEYRKYIEKDAALER
                     RFQPVYVGEPSVEDTIAILRGLKERYEAHHNVRIKDSAIIAAAELSHRYISDRFLPDK
                     AIDLVDEAAASLAIQ"
BASE COUNT         3797 a         4228 c         4743 g         3819 t
ORIGIN      
        1 gattgcggtt gtggttcttg ccggcctctt ttttgccgga ctgattacct ataacaaccg
       61 ttctgagcgc gctgccagcc tttttggaca ggccatggac atctacagca ctccgatcgc
      121 ccagcccggt cagcccgccg agcccggagt aaagacctac gcctctgccg ccgagcgcgc
      181 gaaggccgcg tatccgctgt ttgaagaagt ggccagtaaa tatggatggc tgaaggaagg
      241 cgtaaatgcc cgttatcttg ccggaatgag tgcgctagat atgggccaga catccactgg
      301 cgaaaatgac ctgaagaagg cggccgattc gagcgacagc aatgtggctg ctctggcaaa
      361 gctggccctg gccggaattt accaccagac cgggcgtgac tcgcaggctg ttgcgttgta
      421 ccagcaactg atcgcgaagc ccacagcaac ggtcccggcc agcctggcaa agctacagct
      481 tgcgcagatg tatgagtcca ccaagccgga cgaagcaaag aagctctacg ccgagatcaa
      541 ggaccaggac aagggcacgg ctgccggaca gattgccaca cagaagctgc agggtgcggt
      601 tgcggtcccg cagcaataaa tctgccaaac agccaagttc tacagggccc acattccttc
      661 aggatgtggg cccctcgttt tatgccactg ggcatcctat ttacgtgagc attacgggcg
      721 gtctctgcgt cattcataca taagaaaatg ggtacgcttg aggcacaagc cgctgtctgg
      781 ttttacactc aacctgtctt gagaactcaa gaagaaacag gcgaaattat cactgcactc
      841 gttgccgagt actcaacgat gctctaccgc gtcgcgtatt cggtcatacg aaatgccgcc
      901 gaagcagagg acattgtgca ggaggcattt ctccgcgtcc taaggcacaa ggaaaaactg
      961 aatgagatcc gcgatgcgcg tgtatggctt gtccgcattg cctggaacct ggctctggac
     1021 aggaagcgcc gggccaagac gcgtccggaa acagaagaca tagacgccca tgcgcgcacg
     1081 ttgacgacag cagagcgccg tgctgatcag gaagtgatct cgtcacagga atgcgcacgc
     1141 attctgggac tgattgaccg gctccccaag aaggagcgcg aggcgctgct gctttccgcc
     1201 gttgaggaat tgtccacaac agagattgca gccatttgtt cgacgacgga atcttccata
     1261 cgctcccgca tcttccgtgc gcgaaaggaa ctcgcgttgt tgctggccaa agagagaggt
     1321 ggccaatgaa acatgcattc gacaaaaaga ttgatgcagt ccttcaccgg attgcccatg
     1381 tccagccgga acctggttta gaaaagcgta tccacgcgcg cctggagcgt gaatccgcgc
     1441 ggatggagaa gaaccctgct gtacggctga gaaggttctt tcttgcgcaa agacttgctt
     1501 ttgccgcggc cgcaaccgta gcagggtgcg cggtgatggt cgtgggcagc gtgcagcatt
     1561 cgcggcaaaa agcgctggag ggagtggcgc ctccagctgg aatccatctg ggcgcgcctg
     1621 agagtggcgt aggagctgcc tccggcgtgc gcatttctcc gcagcccatg gttgctcctg
     1681 aaaacactca tgcccggtca gagcgcaaag tcacaaatgg cagggccacg gtcctgaaag
     1741 atgctcataa gcccacagga gtcgcagtcc cagggtcgcc tgagcagaag ccgtaaagcc
     1801 acactctttc aggatgtggg cctctggtct ttagcttgac aacgcctcga agagcatgtg
     1861 cagaatggca agcacggctg ctccgataaa tgctgcgccg aagctgttca cctgaaatcc
     1921 aggaacaacg gcgctggcca tcctgagaat cagcgcgttg atgacaagca gaaacaggcc
     1981 cagcgtcaga atgctgagcg ggagcgtgat gagcttcagc aagaacccga tggttacgtt
     2041 cagaagaccg atcacaatga cagcaaccag cgccgacggc aggttcagta ctcgaaagcc
     2101 tgggaccagc cacgccacaa tcagcagggc aagtgcatca agaaaccatc tcaccaataa
     2161 gtgcaacatt ctgtaaaaaa cgttcctttc gctggaaggt gatgaaacct tccggtcagt
     2221 accagcgtag cgtagcataa gatgctgctc atgaagaatt ttctgcccac gatgttgtgc
     2281 gcgtgtactg caaccatctt tgcgcaatca gcccaggccc cgttgcccga tgttcccacg
     2341 ctgatccggc aggtggaaga gcaccagcac aagctcgacc aagtgcgaga gaactatacc
     2401 tatcacgaga cggccgtcac ccatgagttg gacaagaacg gcaaaatcaa gaagaccgag
     2461 agcgaggaag acgaagtctt ctttgtgaat acgcatgagg tccaccgcaa gataaaaaag
     2521 aacggcaaag agctaagccc ggacgaacag aagaaagaac aagaccgcgt tcagaaagaa
     2581 gttgagaagg cattgaagac gccccctggc gaatctacag acaaacacga ggtttcagtc
     2641 agccgcctgt tgcaaattat gagggtctcc aatccccgtc gcgagcagat ggacggaaga
     2701 agcgtgatca catttgactt cgtgggcgat cctcgcgcta aaacgcatgg cgatgaggcc
     2761 gtccttaaga agatttccgg gaccgtgtgg attgacgaga aagaccggga agtgcgacgg
     2821 ctgacggcgc gctttgacga caactttcac atgggttttg gcctgttttc cattggaaaa
     2881 ggcagtaatc tgacctttga tcagaagctg gtaaacaacg aactatggct ccctacaggc
     2941 gcgcagatcc atattgttgc ccacgcgatt ggagtgattg gatggcgggg cgacatcgct
     3001 gtgacggacg atgattatca gaggttccat gccgatgcct cgcagcaacc gggagcaacg
     3061 gtggtaaggt catctgatcc tcagcattga agtgtatgca aggagaaggc cgcttctgaa
     3121 gcggcctatc tcgcgtgagc aaagattact gctgaggctg ggactggggc tgagactggg
     3181 gctgcgcttc tccgccctga ccacgatgat ggcgttcctg catcctctgc tgaatggctt
     3241 cgtatttctg cttctgctgg tcattcagca cggcttcaat cttgcccttg gtgtcttcgt
     3301 gaatgctctg gacttttgac atcttgtcct ggcgtgagag cgaggtgtcc tgacgcacct
     3361 gctggatctg ctgctgctgg ttttcaagca gcggcttgat ctgcgtctgc tggtctgagg
     3421 tgagattgag tgctttggtc agatgggcca attgtgcatc cgggttaccc atctgatggc
     3481 gagggccgtg catgggtggc gctgtttcgg gttgctgctg agaagaggcg tcctgcgcga
     3541 ttgcggcggt cacgccgaat cccaggagtc cggtgagggc gagcgtacag aatagttttc
     3601 gcattgtgga tgattcctcc aacacatagt cagaacacaa tctgtcgcgc tgggttgcgc
     3661 tgatgtccga caggcgtatc caatgcagat agacgcagga cagccctact gagatgactg
     3721 ccaccggcga atttactgcc tatttaccga gaattttttg tgagaggagg cataacgacc
     3781 ggctccgatc tcagcccaca aggcagcaac ccttctgcat gttcttgagg atgaggcaca
     3841 taggcatagg cgaagatggc ccggtactcg cgttctccgg gatagttgca gatggcgcaa
     3901 cccggacggt acgcaatggc aggatggtct ttgatacttt cggtccattc ctgtgaagaa
     3961 tgtggaggaa cggtcatgta ttcggcgtgc tgcggcggtt gagccggctg ataagcaaat
     4021 acttcgcctt tttcatgatt ggcgtcaacc agactgccgc ctgttccgct ggaagcgcgc
     4081 cataaccatt tttccccgac atgcgcatac caatgcgcgc tggtgggcac aggtttggcg
     4141 aggcggatct ccttatttgt atcgtttttg attacaaata agaaccctgg gcctttgcct
     4201 ggctctgctt cagcaagata actggcgctc aggctgatgt gcgagcagag aggaacagca
     4261 atcccgggat gatgggtcac tgcgcttccg gcagaaacgg caatcatggg gagcagaaag
     4321 aacgcactgc gaaaaagaat tccggacttc agcatcgcct ttgattcaag actaaagcat
     4381 tttttcgttt cactgtgatg cgcgaaaaag ttgaggaaaa tcgcagcaat attggtctac
     4441 gctcacattg ctaagattct tccttcctcc ggtccgacag aatgacggac tatatacacg
     4501 aacgcgcaga ttgcatcttg cctggtctgg tttcgccttc gaaaaagaag cccaccggag
     4561 atactccgat gggcccaggg aggtgtttaa ggttgtcaga aattgattct gccagtgatc
     4621 tggacattac gcggattgtt caatgttccc cccggaacac cgaacaggag atcatttggc
     4681 gctgaatccg gaatggacca ggtatgacgg ttaaaggcat tcagcaattc gaacttcagt
     4741 tggaacacaa tgttctcgtg aatcggcgtg ttcttgagga tgctgaagtc ctcgttgtaa
     4801 tacgcaggcg tgcgcacttc ccctgtcaca cgcggcatat tgcctaaact gaatgcgcca
     4861 tatgcaccgc ggtttctcgc atcgttctgg tcataaaatg ctggatgagg gtctatgatt
     4921 gtgacactcc catccgcatt cacctgggta gcatttgtgg tggagaggtg aaacaaagta
     4981 tttgtttgcg ggtctgcgcg agtggccccg attgggttga tgtgtccgcg atggtatgcc
     5041 gggctcttca ggtcagaacc cgatacacga tcaaaacgta tctggttctg gaagcccggt
     5101 atgccagaag cgcagcagaa cgagaatggc tgtccggaca tatatcgctg taccgcgccg
     5161 atttcccatc ctccgacaac atagctggcg aatccgtggt tcagccaggt tttgccgcga
     5221 ccgaatggaa gatcgtacag ccagctcaga acaacggtgt gcggcacatc ctgcgcgctg
     5281 attgccttct cctgatgcag attgttgacg ttctgtacct gtgccacgcc cacgccgttg
     5341 ttcggtaaca aactgtctga gtttgtgatc tgcttggccc aggtgtagga cgcctgcagg
     5401 ttcaaaccat tgtggaagcg gcgttcgagt gaaaccagca tggcttcata gctggacata
     5461 ccaacgttct gcaggcagca cccgctgtca atgtagtcat attgcgggaa aggccgaagc
     5521 gccctctcta catccgcacc gttccacaat tgattaaagc cggggaaagg ttccggaatt
     5581 cccaacgaat taccagcaaa ggcttcactc aggtggttgc caagcgagaa gtatttctga
     5641 tcaatgttgt tgatgttctg aatgttggag tgcagatttt gtgctttgtt gcctacataa
     5701 ccaaccgtca tgatcaggtc cgttgcaagc tgctgctgca tctgcagggt ccattcgctg
     5761 accatggccg gacggccata ttgcttttca atgtaagaac cgctgacggg tttgccattg
     5821 aagattcccg gatcgagatt cggtggttgc gcgaatgcgg gatagcctgt atccaggcta
     5881 aaggacggat cgaagccatt gtccgggaag ctgggattgg cgcggtagcc cgtattcatg
     5941 ctgccgccaa aatcggaata ttgcagcggc ccataaagga ttccagcccc accacgaaag
     6001 acagtcttgc cttccagttg agaaggagaa taagcaaagc cgatgcgtgg accgatgtcc
     6061 ttgtaccatg tgtctgccca agccgtgttg cagcccttgc agttcgtgcc gaagaccatc
     6121 gcgccgggaa tgttgaactc tggatcgtta gcggtcggac tgaaatttga ggtgaagttt
     6181 ttcgcctcgt gccgtggcat atctacatcc caccgcaggc cgagattcag cgtcaggtcc
     6241 ctggtgacct taaagtcgtc ctgtataaag aggccgtaat accaggatgt ccagcgggac
     6301 tggtgaggaa taatgctctg gcttccaccg caggcaaggc ccaacatctg actggcaaaa
     6361 ccatatccgt cgccaattcc acgggatgaa gatgtctggg tcccacagaa attgatcgtt
     6421 gggctgtttc cgttaatggg agaatactgc tgccagcgga tgtctgtccc gaatgcaagg
     6481 ctgtgacgtc catgctgcca tgccacgctt tcattcagac gcagaccatt atcaatgttg
     6541 tcatcattct gcggcggatt ccctaggttg tccacgttgc cattcgtgat ctggggaaag
     6601 tttttggaat cggcattggc aatgcccaac tgctgcgacc agttcttgtt tgcgaagatg
     6661 gcctgagcat agttgatgct gttgctgcga ttgctgccga agttcagatg gttcagcaga
     6721 ttcggcgtaa aggtatagtc ccagccaaac cgtccaaagt gcgtaaggaa gtcctgcttc
     6781 catgtattcg ggtcaaccgg atcaggaagg attgggttcc cgcccgaagt acggttgttt
     6841 tcacgcgcgc tatacgatcc ccagattctg ctcttttctg tcaggttcgc atcaatgcgt
     6901 actgtatatg tcgtgttgtt gattggaatc gcgctggtga atgcaaaatt attaatctgt
     6961 tgatcgttcg taggcagcgg ataataattc agcaggtttt tggccacagg gctgaaacgg
     7021 ctgggatcaa tcttgtttcc agggaactgg tcgcggcatg tcaccggccc gttgggagtg
     7081 ctgactgtgc gttccgtcaa cgggtcaaaa atttcccctt gatagactgg cgcattgccc
     7141 tggcacggat tggtgcccac tggtttgagg tccgtacgat aaataagcgg acctgagaaa
     7201 tcgccattcc tcatggcaat ggttggaaca gtggagatgt tcgtcgctcc gagcttataa
     7261 ccgagctgct cccaactgaa gaaaaagaag agcctctctc tgccgttata gagctttgga
     7321 atccagacag gaccgcccag tgttacgccg taatcgttct ttttgtcctc aggagtgtca
     7381 tattgttttc tacattcgcg ggtattgttc gatccggagc aatagtaggc gcggttgccg
     7441 ttgttgaacc aagtattcgc gtccaggtct tcgttgcgga agatttcata cgcagtgccg
     7501 tggaaatcat ttgtaccgct cttggttaca aaattttcga ttccacctgt tgtgcggccg
     7561 tattcagccg gaggcaatgc agtcgtgatc ttgaattcct ggagggcctc tacggaagga
     7621 gcttcttcgt caaaagaaga gccattttca ctgcgctgct ggctggcacc gtcaatcagc
     7681 acttcgtttc catagttctg gccaccggca attttggaga ggaagatgcc gttattgcta
     7741 tttgccgtgc ccgggcctgt tgttccaggc aacaggaaca caaatgcttc cggagagcgc
     7801 aatgccccga caccaccgag tgcgagcggg agttctacaa tctgacgcga ggccacgatt
     7861 ccgcctatgt cagaggattc ggtctgaagc gtaggcgcat tcgcgtttac ggtaacagtc
     7921 tgtgccgcgg ccccgggctg gagcgttacg ttcaacgttg tcacctgctg tagctggacg
     7981 agaatgccgg tggaaacaga attctggaat cccgcagcag aaacggtaac gtcataagtg
     8041 cctaatggaa gctcaggaaa gcgatatgcg cccgctcccg tagaaacgac tgtagaaatg
     8101 tcaccggttt tttcgttttt ggcctgcacg gtggcgttgg cgatgactgc cccggaagaa
     8161 tctgtaatgg tcccggacag cgtgccgcgc accgtttgcg ccggcgctgt tccagatgta
     8221 agaatgcccg cccacaggca aatttgcgtc atgtaaatcc aaaacttttg ccgcatcctg
     8281 tcctcctgaa aatgtctttc ggtctgggtg atgtacccca aacccttgat tttctttgtt
     8341 tttcggaagc gcttacattt taagtaagcg cttccatcct gatgcatttc tttctcttct
     8401 tcggctttta aaatcctttt gtttttagcc gtggaagcga ttacatagct tcattttgat
     8461 ataagaaatg aacgacagtt gtcaatgaat tttttaaacg atatacgcaa aaccgatttt
     8521 tccataagag tggcccatcc ggtacactcc catcacagtt cagtgaaagc gagtagtcga
     8581 agtttgcgcc tttgtttgtc cggccaaaca ggtttccggt tttctttcct gctcgcgtgc
     8641 atggccgtgc tttcgggctg ccatgagggg aattatcgcg ttgcagtcat tccccggacg
     8701 acggcgctca ctatctggga ggccgtccac gccggggctg aatttgctgc ccggcctgct
     8761 ggtatccagg tgtactggaa tgctcccacc agcgaagacg atattcagca gcaggcagcg
     8821 ttagtggacc gggtggtgga tgagcattat caggggctgg tgctggcgcc ggaccagccg
     8881 cttgcgctga tgtctcctgt gcgaagggcg attgcccagg gagtacacac tgtggtcatc
     8941 gcttctccgc tgtctattcc tgcgcaggag aacctcgcat acattgtgaa tgatgatgaa
     9001 gccgcagggc gcatagctgc gctgcgtctg gggcaggtgt tgagcgggca tggaagcgtg
     9061 gccgttgtgg gaatcaatcc ggaatcgctg agcgacctga ccatactgca cgcgtttgag
     9121 gcggcgctga aagaacattt tccggacatc acgatcactg acctccgcac cgggacgcat
     9181 aatgttaccg aagcgcagca gattgcggac gaggttctgg ccacgcattc cgggctgggc
     9241 gcaatctttt ctctttctgc gatggccagc aacggcgctg ttactgcgct tgaaaccaga
     9301 ggacttgcag gtaaggtaaa gctgattggc tttgagcaga gcgtggagct gggaaatgaa
     9361 gtccgcgcgg ggacgatgga ttcactgatt gccgaggaca cttatgagat ggggcaccgt
     9421 gctctcagta tgctggcgca gtaccgttcc agcagtctgc cggggcagga aatcagactg
     9481 caaccaagtt tgctgacgcg cgagaacatt gattctccgc tggcgcggcg gctgattaac
     9541 cgcgactggg ggcagaagcc gtgattcgga cgtggttttt tgcggctgtg tgtgtgctgg
     9601 cggcagcaac tgcggtattt tattggcgga gtccgtggcg aggcctgcct tatcaggatt
     9661 cctttgcggc gagaacaagc agtgagtgga agccttatgc cggtaactgg agcgtggtgc
     9721 gtggaggcat caggaatgat tccaatgagc gcggcgcaaa actggttacg ggatcgccgt
     9781 tctggagaga ctactctgta gaagccgaca ttgagctgct gggcgaaggc ggcgatgcag
     9841 gattgatgat ccgcgcaagc gatgtggaac gcggcgtaga ttcttacgac ggctactatg
     9901 ccggtgtgcg aacaacagac cagtctttgg tgctggggcg cgcggagcac ggatggattg
     9961 aatttcctcc agcgaagatg cccggcggtg taaaaccagg cagatggtat cacctgaagc
    10021 tgggcgcctt tggttgcagc atcagcgcct cggcccagga gatgggcaca aacaacaggc
    10081 agaccgttac ggcgcatgat ccaaactgtt tccgctcagg aaagaccggg ctgcgctcca
    10141 tggtggctgg cggcgtctgg cgaaatgtgc gggcagagcg gatttccagc gcggaagcgg
    10201 cgctgttgtc ttcggcaaag tctccggcgc agatggcggt ctacccaaca aaccagggca
    10261 ctctgccagt gccggagaat gtgcctgctc cggttgcaga aggccctcac gagtccggcc
    10321 cggtaacgcc aattcagaac ctgagactgt ttcccgttac gcatgctgca catgctgttg
    10381 tgcgggggac cgtcattctg accgatccgc tgtatgtgca ggactcaagc ggcggagtgc
    10441 aggtggacct gaaaaccaag tcgctgctgc gagtggggga tgaagtagag gtggaaggcg
    10501 atgtgtatcc gcacgggttg actgcaaccc tgcgcgatgc aaaggtgcat tcgctgggag
    10561 ggatggcgcc gaagccgccc ctttcggtga ccgcggacca ggccgcgacc ggcgcgtatc
    10621 atgcgatgtt tcttgaggtg gaaggtacgc tgcaaagcaa ggcggctaca ccggacggca
    10681 tgacgatgct cgaactgagg gatgggcagc aggtctttcg ggcgctgcct gcttcccttg
    10741 ccgtttcggc atctttcaaa aaacttgaga agaacagtct gctgcatctg cgcggcgttt
    10801 gtctggtcaa tgcggcctat acgcagaatg cagttccatt tgcgctgctg gtgagttctt
    10861 ctgaagatgt gaaggtgctg gtgggaccgc cgtggtggag tccggaacac ctgattgcgc
    10921 ttgcactcat catgcttgcc gcggggtatt ttgtgcattt tctctacagt cgcgctgaac
    10981 agtggcgtct gcgcgcagtc attgatgaac gcgagcgtct ggcgcacgag atccatgaca
    11041 ctctggcgca gagctttgcc ggaatcggtt ttcaattgcg cgccattcgc aaccggattt
    11101 caaggagcaa tggccctctg gaccattcca ctctgctgga agagctgaac cgggccagcg
    11161 aactggtacg ccacagccat gacgaggcga ggcggagtat cacgacgctg cgtcctgatg
    11221 ctacggaagc aggcgggctg attgcggcac tcgaacagat tgcgcggcaa atggtggggc
    11281 gcaattccgt gcatattgaa acttcagtgg aaggagaggc gcgcccgatt cctctccgcg
    11341 tgctcgattg tctctttcgc atcgggcagg aatccattgc caacgccatc cagcatgggc
    11401 atccgagcag gctgacgata cgggccgtgt atctgcccac agagatcacg ctggtgattg
    11461 aggacgacgg ctccgggttt gtcccccggc acgattcgga tggttttggc ctttccggca
    11521 tgcgccgccg cgcagaaagt attggcggag agctgaagat tgaaacggcc ccgggaaaag
    11581 ggacgcgcat tgtcgtggac gctccggcgc ctccggaaaa acacagactg ctgcgtattg
    11641 cttatgatga aggaaagcat gtcccgaatc cgcattctga tagccgatga tcatcctgtc
    11701 gtccgggccg gactggcgag catgctggcc acgtatcctg atcttgacgt ggtgggcagc
    11761 gtttcagacg gacagcaggc gattgaaatt ctgaaggcac agccggtgga catactactg
    11821 ctcgacctgc gcatgcctgc gctgaatggc gaacagacgc tgcgcgccat acgccagctc
    11881 gattcacccc cacgcgtaat tgtgctgacc agctatgaga gcgacgagga catctacggg
    11941 gccattcgcg ccggagcaca tggatatctg ctcaaagcct cttcagaaga ggagatgatg
    12001 gaggccatcc acgctgtcta tggcggaggg cggtattttc cgaagcacat tgctgcgcgt
    12061 tttgccgacc gtgtacctcg agcaaacctc agtcggcagc aacaggagat tcttgacctg
    12121 atcgccgagg ggaccactga cacagaggtg gcggaaaagc tggggctcag cgtttccgag
    12181 gtctgggaac ggctgaacac catcattgag acactggacg ccctcgaaga agcccgggca
    12241 caaggcggca aggtgacgat tgccgacatc gcccgcaagg ccggcgtttc gatggcgacg
    12301 gtctcccgtg ttctgcataa taaaggtaag cacacggaag agacgcgccg cgcggtcatg
    12361 aaggtagtgc gggaatacga cttccagatc aacgacaagg cggcttcgct tgccatgatg
    12421 cgcagttctt ctcagcagga ctgaacggtc ttcatagcta agaagcggtc agcacatgct
    12481 tcgcaggccg tcagccaaag ccgttttcgt ttcacttgca tataccaaaa atgctaaatt
    12541 cctgaaaaca tgtgagttgc ccgtaaacaa gaggtcccgg tcgtggatgc gggtaccccc
    12601 tcccctgttt tgtacgggaa ttctttgttt tcaatcactt acaagcgggt gtctgtacgg
    12661 ggatgcttta ttttcaataa gatagctaag aagcggccag cagtcagcgc ctgcttcgca
    12721 ggccgtcagc ccaatgcttt ttcgtttcac ttcgatatac caaaaatgct aaattactga
    12781 aaacatgtga gttgtctgta aacaagaggt cccgatagtg gatgcgggta cccctcccct
    12841 gttttgtacg ggaattcttt gttttcaatc acttacaagc ggatatctgt acggggatgc
    12901 tttattttca ataagatagc taagaagcgg tcagcagtca gcacctgctt cgcacttttt
    12961 cgtttcactt cgatgctata acgctgcaag aatccatggg ggcacccaga aggccgtggg
    13021 tatacataga taaacttggt atacataaaa tcaagtgcga tttttttgta aattcttgat
    13081 aataatatag ttattaaatt atatgctctc tttttcgagt catttttcat acctagattg
    13141 tctactgatg gtcaggagaa tgggcggcag ggttttctcc agtatgttct tgaaaatgag
    13201 ctggtttttc gatgttcttg tatctcattt tcagtgtccg ctgcttccct agattgtcta
    13261 ctgattgccc agaactccgg tacctcgtct tataaagtcc catgcatcct ggtccccggc
    13321 acctgttatt tttgcccttt ctttcaggat atcaggttga gtggaagcaa ccggaccata
    13381 tccatcccct ttatcttatt gaaagcatga aagttaagat gatttgttac cttcgtaagg
    13441 gttgacatga ggtgagattc cctgaaagtg ggccattgtc tcgtgatgta ctaaactctg
    13501 caaaaccgcc tgaattgaaa ctttcactcc agtgatggtt taatttggag cggtaagtaa
    13561 gtgctgagaa caactgaatg aactactcca aaatcgctta ctttttcatg gggcggttcc
    13621 gcaaaaagcg gttgaaaatg ttctctgccg ccttcccgcc ctctacctat cacacagtat
    13681 tggatattgg aggaacgcct cacatctggg acatgttagg cgactcttat gaagtgacgc
    13741 tgcttaacaa tgacaggcgg gagtttgagg ctgggggagg acgttatcaa cactacacat
    13801 gtgtagttgg tgatggctgc catctgcaat tccctgacaa atcctttgat ctggctttct
    13861 ccaattcagt cattgagcac gttggaaact ggggagagat ggaacgtttt gccgcagagc
    13921 agcgtcgcgt tggcaagtca ttctactgtc aaacaccaaa taaatggttt cctgttgagc
    13981 cgcacctggg tactttattt tttcattggt ggccccggct cctgagaatc tatttcgtca
    14041 cccgctatct cactctttgg gggctgatga ataaacccga tcgagagcag gtagaaaaat
    14101 cccttgacaa cattcacctg cttacccggc aagatttaga gcgcctcttc cccggcgcaa
    14161 aaattgtctc tgagcgcttt ttcttcctta ccaaatcata tattgtcgcc ggttgaggct
    14221 actgcatctt atattattca gcagttacgt tcatgtctga aggaactctt gtacaaccag
    14281 cagctcctgt gggcaaagaa aacttgtccg gtcagtgccg ccccattgaa acgaccgata
    14341 ttaagctggc tggatatagc ctgcacagtt gctccaaaac aacgagcatc tactgttcca
    14401 ccagtggttg gtagtgctga aattgctcgc cctcacctgc tgcctgcacc cttctcgtgt
    14461 ggatgttcgg gagaatttat ccgggaagaa acagcaccag tcagcacaaa ctgaaccccg
    14521 cgattttaaa ctttatttca aagtcttaaa aataaataaa gtataaccgc attcccaaaa
    14581 ttgataataa atcactcata ttttatgcaa ccattatgct atagtctgaa tcagatgatg
    14641 tgtaaggcca gaccactggc ctgaaccatt gaaaaatctg gaggaaacta tggccattac
    14701 tcgttgggac ccattccgtg atgtgctggc tctacagaac cgcctgaatt cgctgtttga
    14761 cgatttcacc cgcggtcagg gtgaacagga tgctctgacg acggcggcct ttgtcccccc
    14821 ggtggacatc tatgaggacg accataaggt tgtactgaag ctggaagtcc cgggcatcag
    14881 gcaggacgag ctcgacattc gggttgaaaa caatacgctg accgtccgcg gcgagcgcaa
    14941 atttgaaaag gaagagaagc aggagaactt ccaccggatc gagcggcgtt atggcagctt
    15001 cttccgcgcc tttaccgtgc cgagcacggt ggacacggat tccgtccacg ccagctatga
    15061 tgccggcgtc ctgaaaatcg agatgcagaa gaaggcggaa gcgaagccga agcagatcaa
    15121 agtgaacgtc ggctcttcgc ctaagccaat cgaggctgga aaggcggaga aaactgctgc
    15181 ctgaggtgga acgaacatca aactatgatg gggcggggtg atttcccgcc ttttttgtag
    15241 aagcaagcta ttggttgttc gtccgcagta ttcatcgtga ttcatcacgg aagacaaagt
    15301 tgggagacca acttcgtggg acaggggacg aacatccagc aactaacaac aactggtgac
    15361 tgaattatgg caatccgttg ggacaaattt acggtgaaat cgcaggaggc ggtgcaggcg
    15421 gcaagttcgc ttgcgacaga aaacggcaat ccggagctgc tgccgctgca tctgctcgcc
    15481 gcgctgcttg aagacaagga aggcattatt gtccctgttc tggaaaaagt gggcgtgcca
    15541 acatcgcaac ttctgtcgaa agcgcaggaa gcgattggca ggttgccgaa ggtttccgga
    15601 ggaggcgggc agccgggcat gtctgccgcg ctacagaagg tgctggagca ggcattcaaa
    15661 gaggcagaca actttaagga tgagtatgtc tcgaccgaac acctgttgct ggcgctgacg
    15721 cagcagaaga atgatggcgc gcagttgctg ctggctgcct tcggtgcaac gcacgacgcc
    15781 atcttgaagg ggctgacgac cgtgcgtggc aaccagcgcg tgactgatca gaaccctgag
    15841 gcaaagtatc aggcgcttga gaagtacgca aaggacctga cggaactggc gcggcgcggc
    15901 aagctcgacc ctgtcatcgg gcgcgatgag gagattcggc gcgtggtgca ggtcctggcg
    15961 cggcgcacga aaaacaatcc ggtactgatt ggcgagccgg gcgtgggcaa gacggccatt
    16021 gtggaagggc tggcgcggcg cattgtctcc ggtgacgtgc cggaggtgct gcgcagcaag
    16081 cgtgtgattg cccttgatct tggttccatg ctggcgggag cgaagtatcg cggcgagttt
    16141 gaagaccgcc tgaaggcggt gctcaaggag attgaagaat ccagcggcca gatcattctc
    16201 ttcattgacg agctgcatac actggtgggt gcgggtgcgg ctgaaggtgc gattgatgcc
    16261 tccaacatgc tgaaacctgc gctggcacgc ggcgagctgc gcgcgattgg cgcaacgacc
    16321 ctgaatgagt atcgcaagta cattgagaag gatgctgcgc tcgagcgccg ctttcaaccg
    16381 gtgtatgtag gcgagccaag cgttgaggac accatcgcca ttctgcgcgg cctgaaggaa
    16441 cgctacgagg cgcaccacaa tgtgcgcatc aaggactcgg ccatcattgc ggcggcggag
    16501 ttgtcgcacc gctacatctc agaccgcttt ctgccggaca aggcgattga cctggtggac
    16561 gaggcggcag catcgctggc catccag
//
LOCUS       DASRBV010000318        28085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_427125, whole genome shotgun sequence.
ACCESSION   DASRBV010000318 DASRBV010000000
VERSION     DASRBV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 28085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28085
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427125"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFA02_11745"
     CDS             complement(<1..491)
                     /locus_tag="VFA02_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU10565.1"
                     /translation="MSDSEAPEHSLKKTLGPWSLTALGIGAVIGSGIFTVIGTAIAGE
                     QFDTSSILNTPVLDYLVHHTALVGRPGAGPALALSLVLVAIVCAFTGLCYAELASMIP
                     IAGSAYTYTYATLGELIAWIIGWDLILEYAFSNMAVCVGFAAHIVDLMDWLGIHPSAR
                     WISP"
     gene            complement(615..770)
                     /locus_tag="VFA02_11750"
     CDS             complement(615..770)
                     /locus_tag="VFA02_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10566.1"
                     /translation="MANTKNVESKEERKKLKRAARKKAAPKAKRTAPRGSNKKKVKKL
                     ARGQSKR"
     gene            complement(860..3481)
                     /locus_tag="VFA02_11755"
     CDS             complement(860..3481)
                     /locus_tag="VFA02_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZU10567.1"
                     /translation="MQQLLDKLNPQQRAAVEAVDGPVLILAGAGSGKTRVITHRIAYL
                     IQERGVAPDAILAVTFTNKAAAEMGERVEKLIGHSTLAKPLIATFHSFCVRMLRRDIE
                     ALRIGNEGLTKTFAIYDESDQQNLVKQIMRRMGLDDKQLTPRVVLSRISWAKNHMIDP
                     QEYYLQSADPISEKIAHIFESYRKELRKNNAMDFDDLLLEAVRLLKVSAEVREKYNRR
                     YRYLLIDEYQDTNRPQYELMKLLAGEAKNVCVVGDEDQSIYSWRGADIRNILEFEKDF
                     PSAHVIRLEQNYRSTQIILEAAGAVVARNSQRKGKRLWTAREGGAKIGYYEAPDGENE
                     ALFIADYIQKYLRKAGQDHEDIRIAVLYRTNSQSRLVEEALRRYGISYTMVGGFSFYE
                     RAEIKDLLSYLKLVQNPHDSIALQRVINVPARGIGKATLETLERIALETGTSTWTAIE
                     RALAERLLPTRACIALDSFRKLIEDARAMLTGQLAEKLTADVSGEEAASSSDEDTSFD
                     FGDEVQHGFAFGLEEELGTQGASDVSFNPFEEDSEQKGEIEEVIEERKGFEFRRPGNA
                     ATLPELIRFIIDRSGYIRALEEEGTPEAFSRVENLKELANAAQDAQERGETLDEFLDH
                     AALVSDTDQYDANSRVTLMTLHSAKGLEFPLVLLAGMEEGLFPHSRTLNDAEQMEEER
                     RLAYVGMTRAMDVLVLTRARYRRRYGNDMPEASLPSRFLEEVPPHLLEDLSGGQRPAS
                     SWGGFAASYTQQPSSFRRGQRYDEGFEGSRHYNYEDEDQRPAKQRGRGEDSFDNIAQF
                     FGARGKAVARPKVETAGPAKNTSGLKSGQRVRHPKYGEGVVFRREGDGEDAKVTVQFT
                     RFGVKKLVEKFAQLERI"
     gene            3593..4168
                     /locus_tag="VFA02_11760"
     CDS             3593..4168
                     /locus_tag="VFA02_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZU10568.1"
                     /translation="MSHPNPFRICVFCGSANGTGPAHAQAASELGHKIAVSGAGLVYG
                     GATVGLMGILADAALKGGAEVIGVMPDVLMDREIAHRGLTKLHIVRTMHERKALMAEH
                     ADAFIALPGGYGTLDEFIEIVTWKQLKIHQKPCILLNINGYFDGFLAFLDHAVQQGFI
                     KPENRALIQIAESVESALALAQQSTPQVLRS"
     gene            complement(4158..5024)
                     /locus_tag="VFA02_11765"
     CDS             complement(4158..5024)
                     /locus_tag="VFA02_11765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU10569.1"
                     /translation="MQEGVPSRTAFGVAYRRAAHQLLDERPPVLDDPVAVPILGPDTS
                     AGIIANSEKQRHPFSLAMRAFMVVRSRCAEDHLKRAVASGVRQYVVLGAGLDTFAYRN
                     PFPPERLRVFEVDHPATQEWKRELLASAKISVPETLAFAPVDFERQTLRDGLARVGFD
                     FEQPAFFSWLGVTPYLTLEAFRSTILFIASLPAGTGVTFEYALPREALCERGKLAFDL
                     IASRVKKAGEPFQLFFLPEQMRNELRGVGFGKIEELSSSEINALYFAERKDGLRLYGE
                     AARLITAWQLNF"
     gene            5126..5608
                     /locus_tag="VFA02_11770"
     CDS             5126..5608
                     /locus_tag="VFA02_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU10570.1"
                     /translation="MNFTKSVACMAVMATGLCGCKASKPNALESHAINWVKHHITIGG
                     KNEKNPFPPTAENVNDGKQAFTQYCSVCHGLDGQNTGVPFADTVSPPVPSLASAQVQA
                     YSDGQLKWIIENGIRPSGMPPSQGIFSDDDIWKMVLYIRHLPPKGSLGEPAVYGGESK
                     "
     gene            complement(5683..6078)
                     /locus_tag="VFA02_11775"
     CDS             complement(5683..6078)
                     /locus_tag="VFA02_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HZU10571.1"
                     /translation="MTTETLSGEMAQFDLAREIEGSLRQKPWQSGLYAKTLFKRDDFR
                     TVLITMERGARMKEHHTDGTISVLVIQGAVRVNAQGQTHELKTHGLFTLAPSLRHDVE
                     ALEDAAFLLTISWPPSEKLRSLEHRGYGS"
     gene            complement(6116..7312)
                     /locus_tag="VFA02_11780"
     CDS             complement(6116..7312)
                     /locus_tag="VFA02_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZU10572.1"
                     /translation="MSLRRILSALLFVLLVLSGCGRLRPKPPREYVYVVAKETFLRDR
                     VAAVSNRVANVTNGQRLEVVEHSRRFLKVKTDKGEIGWIEEHGVIDQNTYQQFADLQK
                     QHANDPVIATATLRDELYLHVKPGRQEARFYLLPENDKLQMLVRASVPKPVPPQAVPL
                     PVPKPVRTDGKVKGKPAGPPAQEGPPMEDWWLVRDAQGRVGWTLSRRLDIDVPDAIAG
                     YAEGQRMVGAYQLTTVNDPEAANAPGGQVPEYVTVLGPYKDGLPYDFDQVRVFTWNTK
                     KHRYETAFRQRDLQGYLPVHVGTQTFDKIGTVPVFSFKVATDDTVAIDPATGAAHPAH
                     TNELTYRLEGVIVRKVEPPPPPGAAPPPSPQQQESSRTAPAPAKGRKAGRRHAVSHSR
                     VRHRHH"
     gene            complement(7426..8583)
                     /locus_tag="VFA02_11785"
     CDS             complement(7426..8583)
                     /locus_tag="VFA02_11785"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZU10573.1"
                     /translation="MKPGWRGLALLPLAGVLFATSCGGKFFPKENGGGGNTSSGDYLY
                     AGNTGTSPFTIAAFSIANSKLNTLSGSPYNFGAASPSALAITPKNTFLYVGSTAGGIY
                     VYTINSNGTLTIGNSGNAVATGVFPSALKVDSTGNWLIAAQGVAGTNGSAYLFAINTS
                     TGALTAQGNAVTLDVGTPARIAMTPNNQIVYISLGTGGVDILSFSASSGSLSLSQILR
                     PKNSNNADYGLAIDSSGSYLFVTETGTVVNNTSGLRVLKIASNGGLTELSTSPVQTDL
                     GPVAVMVSTNGYVYVASRTSNVIDGFALSANGALTTLPNSPYTTGTTPVDLAEDNSHT
                     YVAVACSGGTPDLQVFKIDTSASTAGQLLSFATAATASTSPAGASVIAATN"
     gene            8721..9623
                     /locus_tag="VFA02_11790"
     CDS             8721..9623
                     /locus_tag="VFA02_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZU10574.1"
                     /translation="MRADEDFLASDVLRGRGSATRDEHIAAAYIASLFEGFGLQPGGD
                     NGSFLQKAPLPNPLPERVQKHLSQFENVPRTETWNVVGILRGSDTKTQQDAVLLTAHL
                     DHLGVGKPVNGDDIYNGADDDASGTTAVIELARVLGQGKPPKRTVIFALFGSEELGGF
                     GNQYFLEHPPVPLDHIVANLEFEMIGRPDPAVPAGNFWLTGYERSNLGPELAKHGAHI
                     VADPHPEQKFFQRSDNYALARRGIIAQTISSFNLHKDYHHPSDEISKLDFPYMTTVIS
                     SMVKPVLWLANTDWKPEWKPGGKP"
     gene            complement(9672..10223)
                     /locus_tag="VFA02_11795"
     CDS             complement(9672..10223)
                     /locus_tag="VFA02_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZU10575.1"
                     /translation="MQLDQLIDGLPSHAKDLKLNYSSLVRQNTELTPQQLWGTVVASA
                     IATRNPDLTAAALEEGAKQLSPQAMEAAKAAASVMGMNNIYYRFLHLTSNEKYSTMPA
                     RLRMNVMRSHGVDHNDFELWSLAVSAINGCGKCIDSHEKVVREKGVTEETILAVVRVA
                     SVIHAIGTVLDAERVAQSEAVTV"
     gene            complement(10305..10841)
                     /locus_tag="VFA02_11800"
     CDS             complement(10305..10841)
                     /locus_tag="VFA02_11800"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU10576.1"
                     /translation="MLGVGEKFPAFSVTATISTDKNKAFETITHESYPGKWKIYFFWP
                     KDFTFVCPTEIAAFGKLNQEFADRDAQILGGSVDSEFVHLAWRNNHEDLKDLPFPMLA
                     DVKRDLCEQLGILDPEAGVAQRATFIVDPDNVIRFVYVTDLSVGRNPQEVLRVLDALQ
                     TDELCPCNWQKGEATLTV"
     gene            complement(10888..11322)
                     /locus_tag="VFA02_11805"
     CDS             complement(10888..11322)
                     /locus_tag="VFA02_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZU10577.1"
                     /translation="MNAVESREFREVCERAGLAVTHQRQVLFEVLKNMHGHPSPEEVY
                     ARVKRRIPSISLATVYKNIHLFLESGIFREVSLHHGSLRVETNSKPHHHLVCTSCKAI
                     FDIDADDLGLSEKTGTLPGGFLAQRYAVDVLGLCAACQQKAS"
     gene            complement(11426..12280)
                     /locus_tag="VFA02_11810"
     CDS             complement(11426..12280)
                     /locus_tag="VFA02_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZU10578.1"
                     /translation="MSPQFQSALLNWNIPPVATLLLFLTAVLYLRGWLLIGHTRPQQF
                     PVWRLVCFLQGLVALFLAVASPLDTLDGQLLSAHMGQHFLFMTVAPPLLLLGLPQVPL
                     LRALPRSVIRRVLGPLFRMQWPQKTGHFLTSLKPAWLLMNLSYIGWHIPAAYELALRS
                     EKWHNLEHACFFFTSILFWWPIVRPWPDARQKLSWMLLPYLLTSDLINTIISASLCFS
                     GRVLYPTYAVTSPMLGVDPLSDQAAAGAFMWVTNSAVFLLPAFWITIRLLSPSRERRR
                     MSVPRALG"
     gene            complement(12411..13874)
                     /locus_tag="VFA02_11815"
     CDS             complement(12411..13874)
                     /locus_tag="VFA02_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZU10579.1"
                     /translation="MSRLKQIYGWFERRLQLEGPIKEAALHPVPRSTASWWYVFGSAA
                     MTLLMLQIVTGILLAIIYVPSGSEAWKTLDQLNHQLYLGWFLRAMHGWGSNFMVAVVL
                     IHMAQVFMFGAYKFPRELTWVLGVFLLLMTLGMAFTGQVLRFDQDAYWGLGIGASIMS
                     RVPFIGGPLVHLLLGGPIIAGATLSRFFALHVFVIPGLMLLFTGLHVWMVLKLGINEW
                     PMPGRIVRRETYIQEYNELAHRDGVPFVPDALWKDLFFSAGILAAVAVCAVLFGPYGP
                     GGQPDPTIIQTAPKPDFFFLWLFAVLSYLPPQLETPFVLIAPAVGILVLLALPFVAGV
                     GEKSWHRRPVAVLVLITVAVSWGVFTHLGVHTPWSPVMDAWSGDPIPQKLVHRETPLE
                     RQGAVVFQAKQCRNCHSIGGLGGQRGPALDDVATRLTEDQLIRQVLQGGGNMPAYGKN
                     LSPPEVNALVRFLETLKPANQPAAVDASQQAQVQSEK"
     gene            complement(13862..14401)
                     /locus_tag="VFA02_11820"
     CDS             complement(13862..14401)
                     /locus_tag="VFA02_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU10580.1"
                     /translation="MSDGKQVTRRTLLMKIGIAMNAVVGIAIATPVIGYLFSPVKRKG
                     AYNQWISLGSLNQFPEGQTRLAEFINPFKRAQDGDTAKTPCWVRRLSGNKFQVFAINC
                     AHLGCPVRWFPQSELFMCPCHGGVYYANGDRASGPPERGLFQYDYKVVGGELHINAGQ
                     MPTLANEAMARKEKLVCLG"
     gene            complement(14398..15144)
                     /locus_tag="VFA02_11825"
     CDS             complement(14398..15144)
                     /locus_tag="VFA02_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU10581.1"
                     /translation="MNVRQFLSGALLLGGLLLAAGCERFPGNPRVGSEALRPDEVTDF
                     SRLYAQNCSACHGADGQHGPAIALANPEYQAIVDDNTLRDVISNGEPGTLMPAFARSA
                     GGMLTDAQVDALVKGMRERWFKAGYLQGADVPPYKPDKQGEAARGQQVFQAYCSRCHS
                     SGAQPLNAKTSSVTDGSYLALISDQTLRAIIIAGRPDLGHPDWRNAQPGHAMSDQEVT
                     DLVAWMASQRQQTPGQPYPSQEGTARGKKL"
     gene            complement(15141..15989)
                     /locus_tag="VFA02_11830"
     CDS             complement(15141..15989)
                     /locus_tag="VFA02_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10582.1"
                     /translation="MESHEQHGHSGDTIHLPAPTAWPIVLAFGATLIFAGLVTHVVVS
                     LLGAVLLVTACVGWFRQVLPHEAHEDVPVTEHEITIESERTHVTRIEIKEEHRAHLPL
                     ETYPVLSGLKGGIAGGFAMLVPALLYGLIGFHSIWYPVNLLGGAGVAHWTNPTTAEIA
                     TFHPMALVIAIIIHASASILVGLLYGAMLPMLPRHPIILGGIIAPLLWTGLLHATLGI
                     INPAFDERIQWGWFLTSQITYGLVAGWVVAHQSRIRTTQSMPFVVRMGIEASGLHPEE
                     GEDHRR"
     gene            complement(16087..16680)
                     /locus_tag="VFA02_11835"
     CDS             complement(16087..16680)
                     /locus_tag="VFA02_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZU10583.1"
                     /translation="MSTLAVAQAEREQPWIQPSKGRVAMFCLILAESAIFTIFVVAYV
                     YNIGKSLYGPTPQILEVPIWNSIFLLSSSLTIWLAERSVEHGKIKAFGLWWALTFVLG
                     AIFLVGTGIEWHKLIYEDGLTISTNLFGTTFYSLVGLHASHVIIGLIALLTVLLFTIT
                     GHVRKEHAERIQVLALYWHFVDAVWVVVFTVVYVIGR"
     gene            complement(16693..18390)
                     /gene="ctaD"
                     /locus_tag="VFA02_11840"
     CDS             complement(16693..18390)
                     /gene="ctaD"
                     /locus_tag="VFA02_11840"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZU10584.1"
                     /translation="MADSTFPVPGALEAAPHRGRWSQAAFLAWLHDWVVTVDHKKLGI
                     LYMLYSIFFLLVGGVEAILIRVQLIRPDMHFLSPWTFNRVFTMHGTTMVFLVGMTYIF
                     GFANYLIPLMVGARDMAFPRLNALSFWLTFFGGLLLYYSFIGGYGLYGASSAPDTMWW
                     AYSPLTERAFSPGHAPDYWALSLIVTGFGTIGASINVIATALCMRCKGMTLMRLPLFV
                     WLNVVTSFLALLAITPLTAAQVMLLIDRYLGGHFFDTQAGGSALIWAHFFWIFGHPEV
                     YVLVLPAFAIANEVIPVFSRKAIFGYPAMAAASVGIAFVSLGVWAHHMFTIGMTSVGN
                     TFFVLSTMLVGIPTGIKIFNWVATMWGGKIRFATPMLFCTAFLFQFLIAGLTGIILSV
                     APWDWQLHNSYFVIAHFHYVLVGAIVFCIFAGIYYWYPKATGRMLSEKLGKWHFWLFV
                     IGFHVTFDTMHIPGILGMPRSIFTYEADRGWGPWNFVVSIGGFIQAIAVLVFAFNLIY
                     SYFKGRIAGNDPWDAWTLEWATPSPPPAYNFAEDPVVTSRRPLWDIKHPEDPDWRYE"
     gene            complement(18407..19456)
                     /gene="coxB"
                     /locus_tag="VFA02_11845"
     CDS             complement(18407..19456)
                     /gene="coxB"
                     /locus_tag="VFA02_11845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZU10585.1"
                     /translation="MLTEPYRCVKWSVKWFVFLFIVWGGQALFAEGPQSTPSIFAPAS
                     TPAANIYQLSLFVIAITGGIFVTVGSLIAYAAFRFRQHGQDDTSEPAQIYGSTQVELA
                     WTVVPILIVVVLFLTTARMIFAIQDAPKPKSALDVTVVGHQYWWEFNYPKLGIRAVNE
                     MHVPLSDPDHPTPTYLTLLSADVIHSFWVPQLSGKTDLVPNKVNYLWVDPHHPGVYVG
                     QCAQFCGVEHAKMLLRVYVDTPEQFAAWVKNQQQQAVNDPKVAEGKRVFETQACMNCH
                     TIAGTAATGRFGPDLTHLMSRETLASGATTNTPDHLREWIRNPDTFKPGALMPAMQLN
                     DEQLDQVTAYLSTLR"
     gene            complement(19763..20878)
                     /locus_tag="VFA02_11850"
     CDS             complement(19763..20878)
                     /locus_tag="VFA02_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HZU10586.1"
                     /translation="MKLWDAIVVGGGPAGCACAYDLATWGKSVLLLDKAEFPRKKACA
                     GGLTAKTLRALRYSVAPVVRETIQRIVIEGRDSSHILVRSPKNICVMTVREELDAYCL
                     KKTLEGGAEFQRIGAIERIAQNVSGISLFTREAEWRARFLVGADGANSQVRRLTGDTD
                     WFRKGFAIEANLRRDGAKDDLVFDFAPVLDGYGWVFPKRDHLNIGLYSGGQAQTLDRA
                     KLTEYIATRYGTDAAAENFAGQYLGFGAAGHVVSGSRIFLVGDAGGFADPLTGEGIYG
                     AVVSGQAAASAISAALDRNANAGEAFASLTAGLRGNLRIAGRVARSFYAKPESGFRAM
                     RMPFVRSAVLKSYAEGFNLFRLSGTVRRFLPAVVAEQ"
     gene            complement(20891..21667)
                     /locus_tag="VFA02_11855"
     CDS             complement(20891..21667)
                     /locus_tag="VFA02_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU10587.1"
                     /translation="MAKGRTQNQATFHPLDKNGFIPLYYQIQRALMEKIQSGELSEGD
                     PLASEEELSRHYQVSRMTARQALHGLKASGYAFSEKGRGTFVTRPKLEKNIMHLQGFT
                     EEMRKRGMKPSSRLLEQAVIIPTEDLVQKLKLDKNDKVLHLRRLRIADGIPMAIEDSH
                     IPLKQFPGLDRIDFAKHSLYQTLRERYGVRVGYADETIEALAATKEEAELLTIPRKAS
                     ILSITRVIMTTQETPVEAACSRYRGDRYRASIRVPMTAIE"
     gene            21742..22428
                     /locus_tag="VFA02_11860"
     CDS             21742..22428
                     /locus_tag="VFA02_11860"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HZU10588.1"
                     /translation="MTLTAAFPASLKNKLIVSVQAFPGGPLDDTDTLRRMAIAAVRGG
                     AGGLRANGPEHIAAFRPETSLPIIGILKRFNENGPLITPDFASAKAVAEAGSDIIGVD
                     CSAHRPASLEPWPELLARIAKDLRKPVLADIASLDEAIAAEKAGASAVATTMYGYTPE
                     TEGKRSVDWELVERLVAGLSIPVIVEGHVHTPDDARRALDLGAFAVVVGAAITQPEWI
                     TTRFVKAIEE"
     gene            22669..26037
                     /locus_tag="VFA02_11865"
     CDS             22669..26037
                     /locus_tag="VFA02_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10589.1"
                     /translation="MQKRNPHSLWLLLCTLLLVCIPSTAQTVTGSIRGTITDPGGAIV
                     AGAKVIATNQATGVNTEAVTNNAGEYSIRFLQIGSYKLTVEAAGFETASYGPFSLEID
                     QVAKIDLALKVGQASTTVTVNAEAQPILQTENATNGATFTENTINSVPLNGRDFAQIT
                     VFTPGTISTGYGNFGTSNSTERSTTDSNEPNVNGSRQQSNNYLLDGQEINENINNTIG
                     YNPSPDALEQVRVISSNANAEFGNVNGGEVVMVMKSGSNSFHGSAFGFLQNYNLDANS
                     WKNKNNVVPVPKNPYTQTIFGGTLGGPIIKDKLFFFVDYQGVREHTGGQSTFSLAPPA
                     FLKGDLSAVKNVLGIQLYDTQHLTNGQPTPYPNNQVPVNNPVALFLAAHPEAYPAPNN
                     PTSDPLGIFNNYVGPSKNFVSNDQGDVKIDWHLHNGDVISSRYSQGYAQNGTVTDPVP
                     VIFPSASNYPDHLGNITWTHSFSPAVVNYARASYARIQFNSGVTTDPSGIFGFDGNAK
                     VGIPSKAQLTQGFSQQSFSTNNTNGYPQNFGTNPTPEVFVDNVFEYADNLTWQRGRHL
                     FKFGVEFTRYQQNSFYPGNDGELGNFNYNGQYSALPGSTNLYPFADFLLDRSNDVAIG
                     NVTGLTGQRQWRDGIFAQDDFKVTPTFTLNLGVRWEYDQPIYEVNNKMANINMTTKQI
                     EYAGVDGNSRALYDPYYAQVQPRFGFAWQPSPRWVIRGGYGITSYLEGMGANLRLTQN
                     PPFHTDFEEQGTAPSGSGSSYNPGVYYSASDGFPTTTPPTTTFYVWPKDLKPSSTQEI
                     TLTTEYEVTPSSSLQVGYVGILGHHLTNPYWGNQLPAPGATAPYANIVGQSGVIKISA
                     TNAASNYNALQAVYRQRLTAGLELTANYTYAKSLTDSFGYYGVSNISGQYYQQNAYDM
                     AAEWGPAGFDIRHALSVSGEYDLPFGRGRQFGGGMNRLLDAVIGGWKLAGTNVTYTAF
                     PVTAGSPANYSSLVHAATARPNQYRPLKIVNRSLNAYFGTAVQGTACGAAPNLGPNAG
                     TCIFGAQPTDTFGNVRPMSLRGPGYEQIDMSVSKSFTLWKEHTLNFRSDFFNAFNIAS
                     YSAPDNNVTDASFGQITNTTSTERHIQFGLKYSF"
     gene            26144..27262
                     /locus_tag="VFA02_11870"
     CDS             26144..27262
                     /locus_tag="VFA02_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZU10590.1"
                     /translation="MSRSFPHAMLREIYEQPAALSSTIQNYVSDQALKPEAFAAVADA
                     LRGHQRVVIAASGSSRHAGLAGEIMLEDLAGLTVDVEYASEYCYRSTHTLQDPGVIVI
                     SQSGETADTLAALREGKSRGLSTVAITNNPNSTMAREADASLPTIAGKEKAIPATKSF
                     TTQLAVLYLLTLSMARLRGRMTAHSVSALIEQLEALPGLLERSLPAWEEQLRAVAEHT
                     RNARGFLYLGRGIHYAIAREGALKLKESAYIQAEGYPAGELKHGPNALVTKDAPLVML
                     ATQDRNDPDSVLRYEKTLQLMKDMQAQGAEILALASEGDSEVSRVARFTITVPNASEY
                     LLTILEVVPLQLFAYFTAILHGVDVDSPRNLVKAVVQE"
     gene            27537..>28085
                     /locus_tag="VFA02_11875"
     CDS             27537..>28085
                     /locus_tag="VFA02_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10591.1"
                     /translation="MKLKDLPFSISVSGAVVAALLVAGCARQPSAANDVQAARQAQQQ
                     ARAAEKAQMDAAREALDAIPLPAKSRYMAVRNTESWSNPFLIVGSENVTLRVVYPDVT
                     HNNALPSTMLKPAKARRQELTIRLSDLPDALSSLPNDEWPYGRVVAVEEDPLETRANR
                     LQVRRNIETTMQVLNNLGVVVYE"
BASE COUNT         6305 a         8292 c         7613 g         5875 t
ORIGIN      
        1 ggcgaaatcc atcgtgccga tgggtgaatg ccgagccagt ccatcaggtc cacaatatgg
       61 gcggcaaaac ctacacacac tgccatgttg gagaaggcgt attccagaat caggtcccag
      121 ccgatgatcc aggcaatcag ctcaccgagg gtggcatagg tataggtgta ggccgatcct
      181 gcaatcggaa tcatggaagc aagttcggca tagcagagtc cggtgaaggc gcagacaatc
      241 gccaccagaa caagggacaa agccagcgca ggccctgctc ccgggcgtcc caccagagcg
      301 gtgtgatgta ccagataatc cagaacggga gtattcagga tagacgaggt atcgaactgc
      361 tcgcccgcta tcgccgttcc aatgacggta aagatgcccg aaccaatgac cgcgccaata
      421 cccagcgcgg taagagacca cggccccagc gttttcttca ggctatgctc gggcgcttcc
      481 gaatcgctga tgagcttgtc tatggatttt cgggcgaaaa cttgatgcga caggggtctg
      541 gctcctgagg cgagtgtacc tgctcaaagc ctgccctgcg aagtaaattc ggcagaacag
      601 gctcgtggca agtattaccg cttgctctgg cctcgggcca gcttcttcac ctttttcttg
      661 ttcgatccgc gcggagcggt gcgctttgct ttgggggcgg ccttcttgcg cgccgcgcgc
      721 ttcaattttt tgcgttcttc cttgctttca acgttctttg tatttgccat tcttctcctt
      781 aaaacaagat ccaggcccaa caccagcccc acgttttagt catcctatgc ggagcaaagc
      841 ggagtcgcag gacctgcatc taaatccgtt caagctgggc aaatttctct accagctttt
      901 ttacaccgaa ccttgtaaat tgtaccgtga ctttggcgtc ttccccatcc ccttcgcgac
      961 ggaagaccac gccttcccca tattttggat gccggactcg ctgtccactc ttcagccccg
     1021 aggtgttttt cgccgggccg gcagtctcca cctttggcct cgccacggct ttcccgcgcg
     1081 ccccgaagaa ctgcgcgatg ttgtcaaagg aatcctcgcc acggccacgc tgttttgcag
     1141 gacgctggtc ttcgtcctca tagttatagt ggcggctgcc ttcaaagccc tcgtcgtagc
     1201 gctgtccgcg ccggaacgaa gacggttgct gcgtatagga agcagcaaat ccgccccagc
     1261 tcgaagccgg acgctgccct ccgctcaaat cttccagcag atgcggcggc acctcttcca
     1321 gaaaacgcga cggcagagag gcttccggca tgtcgtttcc ataacgcctg cggtaacgcg
     1381 cccgcgtgag gacgagcaca tccatcgcgc gcgtcatgcc tacgtaggcc agccggcgct
     1441 cctcttccat ctgctcagca tcattgagcg tgcgcgaatg cgggaagagc ccttcctcca
     1501 ttccggcaag caggaccagc ggaaactcca gtcccttcgc ggagtgcagg gtcatcagtg
     1561 tgacgcgcga gttggcatcg tactggtcag tgtcgctcac cagcgccgcg tggtccagaa
     1621 attcgtccag cgtttcgccg cgctcctgcg cgtcctgcgc ggcattggcc aattccttga
     1681 ggttctcaac acgtgagaag gcctcgggcg taccttcttc ctcgagcgcg cggatatatc
     1741 cgctgcggtc aataatgaag cggatcagtt cagggagagt ggccgcattt ccgggcctgc
     1801 ggaactcgaa gcctttcctc tcctcaatga cctcctctat ctcacctttc tgctctgaat
     1861 cttcctcaaa tggattgaag gacacatcgg acgctccctg agtgcccagt tcttcctcca
     1921 gaccaaacgc aaacccatgc tgcacttcgt cgccaaagtc aaagctcgtg tcctcgtcgg
     1981 aagaagatgc agcttcttcg cctgatacat ctgccgtgag cttctctgcc agttgcccag
     2041 tgagcatggc ccgcgcgtcc tcaatcagct tacgaaagct atcgagcgca atacaggcgc
     2101 gtgttggcag gagtctctct gcaagcgcgc gttcaatggc cgtccatgtg cttgttccgg
     2161 tttcaagcgc gatgcgctcc agcgtctcta gtgtggcttt gccgatacca cgcgccggaa
     2221 cattgatcac ccgctgcaaa gcaatcgaat catgagggtt ctgcaccagc ttcagataac
     2281 tgagcaaatc tttgatttcc gcgcgttcat agaacgaaaa cccgcccacc atcgtatagc
     2341 tgatgccata ccgccgcagc gcttcttcca caaggcgcga ctgtgaattc gtgcggtaga
     2401 gcacggcaat acgaatgtcc tcgtggtcct gccctgcctt gcgcaaatat ttctggatgt
     2461 aatcggcgat gaacagcgct tcattctcgc catccggagc ttcgtaatag ccaatttttg
     2521 cgccaccttc gcgcgccgtc cacaggcgct tgcccttgcg ttgcgagttg cgcgccacga
     2581 ctgcgcccgc cgcttccaga atgatctgcg tagagcggta attctgctcc agacggatga
     2641 catgcgcgct gggaaagtct ttctcaaact caagaatgtt gcggatgtct gcgccacgcc
     2701 aggagtagat gctctggtct tcatctccta ctacgcagac attcttcgcc tcgcccgcaa
     2761 gcagcttcat caactcgtat tgcggacggt tcgtgtcctg gtactcatca atcaacagat
     2821 aacggtagcg ccggttgtac ttttcgcgca cctcggcaga gaccttcagc agccgcaccg
     2881 cctccagcag caggtcatcg aagtccatag cgttgttctt acgcagctct tttctgtagg
     2941 actcgaagat gtgcgcgatc ttctcgctga ttggatcggc tgattgcagg tagtactcct
     3001 gcggatcaat catgtggttc ttcgcccagg aaatgcgcga aagcaccacg cgcggcgtta
     3061 actgtttatc atccagtccc atgcgccgca tgatctgctt caccaggttc tgctggtcgc
     3121 tttcgtcata gatggcgaag gtcttcgtca gcccctcatt gccaatacga agcgcttcga
     3181 tgtcgcgccg cagcattcgc acgcagaatg agtggaacgt agcaatcaga ggtttggcca
     3241 gcgtcgagtg cccgatgagc ttctccactc gctcgcccat ctcggcggcc gccttgttgg
     3301 tgaacgtgac agccagaatt gcatccgggg ccacgccgcg ctcctgaatc agatacgcaa
     3361 tgcggtgcgt gatgacacgt gtcttgcctg atcctgcgcc ggcaagaatc agcaccgggc
     3421 cgtccacggc ctctaccgcg gccctctgct gcggattcaa cttatcgagt aactgctgca
     3481 aaatggtccc tggaattgtt tcggtaggag ttctctccat ctagtgtatc gctgccgatg
     3541 tcctgcggtt gctcagaccg gcctttcagc aaaaccagtt acactttcct tgatgtcaca
     3601 ccccaatccc ttccgcatct gcgtcttctg tggctccgcc aacggtactg gtcctgccca
     3661 tgctcaggcc gcaagtgagc tgggccacaa aatcgctgtc tccggagcag gtctcgtcta
     3721 tggcggagcg accgttgggc tgatgggcat ccttgccgac gccgcgctca agggcggcgc
     3781 cgaagtcatc ggcgtcatgc ccgatgtcct catggaccgg gagatcgcgc accgcgggct
     3841 gacgaaactg cacatcgtca gaacgatgca cgagcgcaaa gcactgatgg cagaacatgc
     3901 cgatgccttc atcgctttgc ctggcggata cggcaccctt gacgagttca tcgaaatcgt
     3961 tacatggaag cagctaaaaa tccatcaaaa gccgtgcatt ctgctgaata tcaatggcta
     4021 ttttgacggg tttctggcat ttctcgacca tgccgtccag caaggcttta tcaaacctga
     4081 gaaccgcgca ttgattcaga ttgctgagag cgtggaatcg gcacttgctc tggcacagca
     4141 gtccacaccg caagtgctca gaagttgagc tgccaggccg tgatcaatct ggctgcctct
     4201 ccatagaggc gcagaccgtc tttcctttca gcaaaataaa gcgcgttgat ttccgatgaa
     4261 gaaagctctt cgattttgcc aaagcccacg ccgcgcaatt cgttgcgcat ctgttccgga
     4321 agaaaaaaga gctgaaatgg ctcgcctgct tttttcacgc gcgacgcgat caggtcaaaa
     4381 gcaagttttc ctctttcgca cagggcttcg cgcggaagcg catattcaaa tgtgacgcct
     4441 gttcctgcgg gcagcgaagc gataaaaaga attgtggaac gaaacgcctc aagagtgaga
     4501 tagggggtga ctcccaacca tgaaaagaat gctggctgct cgaaatcgaa cccaaccctc
     4561 gccaggccgt ctctgagtgt ctgccgctcg aaatccacag gcgcgaacgc gagtgtttcg
     4621 ggaacagaaa tctttgctga agccagcagt tcgcgcttcc actcctgtgt tgccggatgg
     4681 tctacttcaa agaccctgag ccgctcggga ggaaatggat tgcgataggc aaacgtatca
     4741 agcccggcgc cgagcaccac atattgccgt acgccgcttg ccacagcccg tttcagatgg
     4801 tcttccgcgc agcggctgcg gaccaccatg aaagcgcgca tggcgagcga aaaaggatgc
     4861 ctttgttttt cgctgtttgc gataatgccc gctgaggtgt ccggtccaag aatcgggaca
     4921 gccaccgggt catcgagaac cggcgggcgc tcatcgagca gttgatgcgc agcgcgccgg
     4981 tacgcaacac caaaggctgt gcggcttgga actccttcct gcatgtggcc ttgcttttga
     5041 tgctatcgca agtactttca tttcagcgcg gcaccccatg aaagaataaa aacgcggttt
     5101 tcccgtttta aattccggag gcagcgtgaa ctttacgaaa tcggtcgcct gcatggcggt
     5161 catggcaaca ggattgtgcg gctgcaaggc gtccaaaccc aatgcactgg aaagccatgc
     5221 cattaactgg gtcaagcacc acatcacaat tggcggcaaa aacgaaaaga acccatttcc
     5281 tcctacagcg gaaaatgtga atgatggcaa gcaagccttc acgcaatact gctcggtctg
     5341 ccacgggctg gacggtcaga acaccggcgt tccgtttgcc gacaccgtct cgcctccggt
     5401 gccgtcgctt gcgtccgcac aggtgcaggc ctattccgat ggacagctga agtggatcat
     5461 tgaaaatggt attcgcccct ctggcatgcc tccatcgcag gggatatttt cagatgacga
     5521 catctggaaa atggtgctat acatccggca tcttccgccc aagggcagtc tgggcgaacc
     5581 tgcagtatat ggtggagaaa gcaaataaaa gatttggcac atccggccat accctgtccg
     5641 gagttggtct aagggagatt tgcagcatcc gtgtacgcaa cttcaagagc cgtaaccgcg
     5701 atgctccagc gaacgcagct tttcacttgg cggccatgaa atggtcagca ggaatgcggc
     5761 atcttccagc gcttcgacat cgtgtcgaag ggaaggcgca agcgtgaaga gaccgtgggt
     5821 cttaagttca tgcgtctgcc cctgcgcatt gacgcgcacc gcgccctgta tcacgaggac
     5881 agagatcgtg ccgtccgtat gatgctcttt catgcgcgcg ccgcgctcca tggtgatgag
     5941 caccgtgcga aaatcgtcac gcttgaaaag tgttttggcg taaaggccgg actgccacgg
     6001 cttctgccgc aatgagcctt caatttcacg cgcaagatca aattgcgcca tctcaccgga
     6061 gagagtttct gtggtcatga gcaccgctcc ttcagaaata gagatgcggt tttccttagt
     6121 gatgtctatg ccggacccgc gaatgggata cggcgtgcct tctgcctgcc tttcgccctt
     6181 ttgcaggcgc tggagctgtc ctggacgatt cctgctgctg gggtgaagga ggaggcgcag
     6241 ctccgggtgg gggcggtggt tccactttgc gcacaattac gccttcaagc ctataggtca
     6301 gttcattcgt atgcgcgggg tgcgccgctc ccgttgccgg atcaatggcc acggtatcgt
     6361 ctgtggccac tttgaaggaa aagacgggga cagtgccgat cttatcgaac gtctgcgtac
     6421 ctacgtgaac aggcagatat ccctgcaggt cccgctgccg gaatgccgtc tcatagcggt
     6481 gcttcttcgt gttccaggta aagacgcgca cctggtcaaa atcgtagggc aggccatctt
     6541 tgtacgggcc aagaacggtc acatattcgg gcacctggcc gccgggtgca ttggctgcct
     6601 ccgggtcgtt gaccgtcgtc aactgatagg cgccaaccat ccgctggccc tcagcgtagc
     6661 cggcaatggc gtcgggtaca tcaatgtcca ggcggcggga aagcgtccat cccacacggc
     6721 cctgagcgtc acgcaccagc caccagtctt ccatcggtgg cccctcctgg gccggcggtc
     6781 cagccggttt gcccttgacc tttccatctg tcctgaccgg tttgggaaca ggcagcggaa
     6841 cggcttgagg cgggaccggc ttgggcacag aagcgcgcac caacatctgc aacttatcgt
     6901 tttccggcag aagataaaac cgtgcttcct gacgtccggg ctttacgtgc agatatagct
     6961 catcgcgcag tgttgccgtt gcaatcaccg gatcattcgc atgctgcttc tgcaaatctg
     7021 cgaattgctg atacgtgttc tggtcaatga cgccgtgctc ttcgatccag ccaatctcgc
     7081 ccttgtcagt ctttactttc agaaaacggc gggaatgctc cacaacctcc agccgctgcc
     7141 cattggttac gttggctacg cgattggaaa cagcggcaac ccggtcgcgc agaaaggtct
     7201 cttttgcgac aacgtagacg tattccctcg gcggcttagg gcggaggcgg ccgcatcctg
     7261 aaagaaccag cagaacaaaa agcaatgcag aaagaatccg gcgcaaagac atgggcaaaa
     7321 tcaggggaga ggcgcattct gcgcgcgttt cctctgttga tttgagaata gcatcgaaat
     7381 acgccgtatt ctgctcgcgt gaatgctgtt ggtaaatatc aggccctagt tcgtcgcagc
     7441 aatcactgat gcgccagcgg gagatgtgct tgccgttgcc gccgtagcaa acgagagcag
     7501 ttgcccggca gttgaagcag aagtgtctat tttaaagacc tgcaaatcag gtgtgcctcc
     7561 ggaacacgcg accgccacat aagtatgcga attgtcctca gccaggtcca cgggagttgt
     7621 gcccgtggtg tatggcgaat tgggcagcgt ggtcaaagcg ccattggctg aaagcgcgaa
     7681 tccatcaatc acgttgctcg tgcgagacgc tacataaaca tagccgttgg ttgaaaccat
     7741 cacagccact ggccccaggt cggtctgcac cggcgatgta gagagttcgg taagccctcc
     7801 attgcttgca atcttaagaa cacgcaggcc gcttgtgttg ttgactaccg tgcccgtctc
     7861 cgtaacaaag agatagctgc cgctggaatc aatcgccagc ccataatcgg cattgtttga
     7921 gtttttggga cgaagaatct gtgagaggct gagtgaccct gaacttgcac tgaagctcaa
     7981 aatatccacc cctcccgtgc ccagcgaaat gtagacgatc tggttgttcg gagtcattgc
     8041 aatacgtgcg ggagtgccaa cgtcgagggt aacggcattg ccctgtgccg tgagtgcgcc
     8101 cgtgctggtg ttgatcgcaa aaagatatgc agacccattt gttccggcga cgccctgcgc
     8161 agcaatgagc cagttcccgg ttgagtccac tttcagagcg gaaggaaaga cacctgtggc
     8221 tacagcgttt ccgctgttac ctatcgtgag agtgccgttg ctgttgatgg tgtagacata
     8281 aattccgcca gccgtgctgc cgacatacaa aaatgtgttc ttcggtgtaa tggccagtgc
     8341 cgaagggctt gccgcgccaa agttataagg cgatccggaa agagtattca gcttgctgtt
     8401 ggcgatggaa aaggccgcaa tcgtaaaggg actggtgcct gtgttgccgg cataaagata
     8461 atcgccgctg cttgtatttc cgcctccgcc gttctctttg gggaaaaact tgcctccgca
     8521 tgaagttgca aacaaaactc cagccagagg cagcaatgcc agacctctcc atccaggctt
     8581 cattctcacg gcctcactca acagatatac ttccctctat acgtatgcga atccacttcc
     8641 gccttgctct gttgatatca ctgctggtcc ctttttgtcg cgccgcgttg gctgaaggtc
     8701 cccatttctg cgacgcatgc gtgcgtgccg atgaagactt tctcgcctct gacgtgctgc
     8761 gtggccgcgg cagcgccacc cgcgatgaac atatcgccgc tgcgtacatc gcttctctct
     8821 tcgaaggctt tggccttcag ccaggcggag acaatggcag cttccttcag aaggcccctt
     8881 tgccgaatcc gctgcccgag cgcgttcaaa agcatctctc tcagtttgaa aacgtcccgc
     8941 gcacagagac ctggaacgtc gtcggcatcc tgcgcggctc cgacacaaag acgcagcagg
     9001 acgcggttct gctcacggcg cacttggacc atctgggggt gggaaagccc gtcaacggag
     9061 atgacatcta taacggcgcg gacgatgacg catcaggaac gaccgcggtc atcgaactgg
     9121 caagagtgtt gggccagggc aaacccccga agcgcactgt gattttcgcc ctcttcggct
     9181 cagaagagct gggcggcttc ggcaaccagt actttcttga acacccgcct gttccactcg
     9241 atcacatcgt cgccaacctg gaatttgaga tgattggacg gcccgatcct gccgtgcctg
     9301 ccggaaattt ctggctgacc ggatatgagc gcagcaatct cggcccggaa ctagccaagc
     9361 atggcgcgca tatcgttgcc gacccgcatc cggagcagaa attttttcag cggtcggaca
     9421 attacgcgct ggcccggcgt ggcatcatcg cccagaccat ctccagcttt aacctgcaca
     9481 aggactacca ccaccccagc gatgagataa gcaaactgga ttttccctat atgacaacgg
     9541 tgatttcatc catggtgaag ccggtcctgt ggctggcgaa tacagactgg aaaccggagt
     9601 ggaagccagg cggaaaacca tagaggtaac gcctgcccca tatctcaaag agacctgggc
     9661 tttgcccagg cctacaccgt cacagcttca ctctgcgcca cgcgctcggc gtcgagcact
     9721 gttcctatcg catgaatcac tgacgccacg cggacaacgg cgaggatggt ctcctccgtc
     9781 acgccttttt cacgcacgac cttctcgtgc gagtcaatgc acttcccgca tccgttgatg
     9841 gcggagacgg ccagcgacca cagttcgaaa tcattgtggt ctactccatg cgagcgcatc
     9901 acgttcatgc gcagacgcgc gggcatggtg gaatactttt cgttcgaggt aaggtgcaga
     9961 aagcgatagt agatattgtt catccccatc acggatgccg ccgccttcgc cgcttccatc
    10021 gcctggggag aaagctgttt tgcgccttct tcgagtgctg ctgcggtcag gtcaggattg
    10081 cgcgtagcaa tggcgctggc cacaaccgtc ccccagagct gctgcggggt cagttcggtg
    10141 ttctgccgta ccaggcttga gtagttcagc ttcaggtcct tggcatgact gggaaggccg
    10201 tcaatgagtt ggtctagttg cataaagtct ccctctgcgc gtttcagcgc ctgatagaaa
    10261 tggcggggca aaggccccgc ctcgttttcc tgaacaacaa gtctctagac cgtcagagtg
    10321 gcttcgccct tctgccagtt gcaggggcaa agttcatcgg tctgcaacgc atcgagcaca
    10381 cgcagcactt cctgcggatt gcgccccacg gaaagatcgg tgacatagac aaagcggatg
    10441 acgttgtccg ggtctacgat gaaggttgcg cgctgtgcca ctccggcttc gggatcgaga
    10501 atgccgagct gttcgcaaag gtcgcgcttc acatcggcca gcatcggaaa gggcaggtcc
    10561 ttcaggtctt cgtggttgtt gcgccacgct aggtggacga actcagaatc cacgctgccg
    10621 ccaagaatct gcgcatcgcg gtctgcgaac tcctggttca gtttgccaaa tgcggcaatt
    10681 tctgtaggac acacaaaggt gaagtctttc ggccagaaaa agtagatctt ccacttgccg
    10741 ggatagctct cgtgcgtaat ggtttcaaat gccttgttct tgtcagtgct tatagttgcg
    10801 gttacggaaa acgcaggaaa tttctcgcct acaccaagca tgtatgactc cttgaaaacg
    10861 ttaagttgtt atgaattgca gcgaaactta cgaagctttc tgctggcatg cagcgcacag
    10921 gccgagcacg tccacggcat agcgttgtgc cagaaatcca cctggcagcg tcccggtctt
    10981 ttcagaaaga cccaggtcat ctgcatcaat gtcaaagatc gccttgcatg acgtgcatac
    11041 caggtgatga tgcggtttgc tgttggtttc cacgcgaagc gagccgtgat gcaggcttac
    11101 ctcgcggaaa attccactct ctaagaagag atgaatgttt ttgtaaaccg ttgctaaaga
    11161 aatcgaaggg atcctgcgct tcacacgcgc ataaacctct tccgggctgg gatgcccgtg
    11221 catgttctta aggacttcaa agaggacctg ccgctggtgc gtcaccgcca gtccggcgcg
    11281 ctcgcagacc tctctgaact ccctgctttc aaccgcattc atcagtttct attaaacgat
    11341 aattattatt gtttgtcaaa gaggccggaa actcctcttt tcatacacac agaagccgaa
    11401 aaattgagcc ctctggccac cggccctaac ccagggcccg tggaacactc atccgtctcc
    11461 gctcacgact gggtgacagc agccggattg tgatccagaa ggcaggaagc agaaatacgg
    11521 ctgaatttgt gacccacata aacgctcccg ctgccgcctg gtccgagaga ggatccacgc
    11581 cgagcatggg cgatgtgacc gcatacgtcg gataaaggac gcgtcccgaa aagcagagcg
    11641 aggcggagat gatagtgttg ataaggtctg aggtaagaag atacggcagc agcatccagc
    11701 ttagcttctg ccgcgcatcg ggccacggac gaacaatcgg ccaccagaaa agaatactgg
    11761 tgaagaaaaa gcacgcgtgc tccaggttgt gccatttctc tgagcggagc gcaagttcat
    11821 aggcagccgg aatgtgccat ccgatatagg acaggttcat cagcagccac gcaggtttca
    11881 gactggtgag gaaatgcccg gttttttggg gccactgcat ccggaagagc ggaccgagga
    11941 cacgccggat gacggaacgc ggaagtgcgc gcagcaaggg tacctgcggc agtcccagca
    12001 gaagcaacgg cggcgccacg gtcatgaaca ggaaatgctg ccccatgtgg gccgagagta
    12061 gttggccgtc gagcgtatcg agcggcgaag ctacagccag aaacagcgca accagaccct
    12121 gaagaaaaca gaccaggcgc cagacaggaa attgctgcgg acgtgtatgc ccgatgagca
    12181 gccatccgcg aagatagagg accgcggtga ggaacagaag gagcgtggcc accggaggga
    12241 tgttccagtt gagaagcgcg gattggaact gcggtgacat ttacgttcta tgattcgccc
    12301 acatctcgaa ccaggccccc aacacgtgtt tttttggcgt gttggggtgg aaagagatgt
    12361 ggggcacccg gtgtgtcatc ctgttctgaa cgcagtggaa ccagcttttt ctacttctct
    12421 gactggacct gcgcctgctg cgaggcgtct acagcggcag gctggttcgc gggcttcagt
    12481 gtttcaagaa agcgcacgag cgcgttcact tccggcggac tcaggttctt gccataggcc
    12541 ggcatgttgc cgccgccttg cagcacctgc cggatcaact ggtcttctgt gagccgcgta
    12601 gccacatcgt cgagagcggg gccacgctgt ccgccgaggc cgccgataga gtggcagtta
    12661 cggcactgct tggcctgaaa gaccaccgcc ccctgccgct ccagaggcgt ctcacgatgg
    12721 accagttttt gcggaatcgg gtcgccgctc catgcgtcca tgacagggct ccagggtgta
    12781 tgcacgccca gatgcgtaaa gacgccccat gagaccgcga ccgtaatcag caccagcacg
    12841 gcaaccggac gtcggtgcca actcttttct ccgactccgg caacaaacgg cagcgcaaga
    12901 aggaccagaa tgccgacggc cggagcgata agcacaaacg gcgtctcaag ctgcggcggc
    12961 aggtaagaca gcacggcaaa gagccacaga aaaaagaagt ccggcttcgg agcagtctga
    13021 atgatggtcg ggtcgggctg cccgcctggt ccatacggcc cgaaaagaac ggcgcacacg
    13081 gccacagccg caagaatccc tgcggagaag aagaggtcct tccataaagc gtcgggcacg
    13141 aacgggacac cgtccctgtg ggccagctcg ttgtattcct gaatataggt ctcgcggcgc
    13201 acgatgcgtc cgggcatggg ccattcgttg atgcccagct tcagcaccat ccaaacatgc
    13261 agcccggtaa aaagcagcat caggccgggg atgacgaaga catgcagcgc aaagaagcgc
    13321 gagagcgtgg cgccggcaat gatgggaccg cccagcaaca gatgcaccag gggcccgccg
    13381 ataaacggca ctcggctcat gatggaagcg ccgatgccca gtccccagta ggcgtcctgg
    13441 tcgaagcgca ggacctgccc cgtgaaggcc atgcccagcg tcatcaacag cagaaagacg
    13501 ccgaggaccc aggtcagttc gcgcggaaat ttataggcgc caaacatgaa gacctgcgcc
    13561 atatgaatga gcacgacggc aaccatgaag ttggatcccc agccgtgcat ggcccgcaga
    13621 aaccatccga gataaagctg atggttcaac tggtcgaggg tcttccaggc ttcgctgccc
    13681 gagggcacat agatgatcgc cagcagaatg ccggtaacga tctggagcat cagcagcgtc
    13741 atggcggcgc tgccaaacac ataccaccag ctcgcagtgc tgcgcggaac aggatggagc
    13801 gcggcctctt taatagggcc ttcgagctga agacggcgct caaaccagcc gtagatttgt
    13861 ttcagccgag acataccagc ttctccttcc gggccatggc ttcattggcg agggtgggca
    13921 tctgtccggc gttgatgtgc agttcgccac caacgacctt atagtcgtac tggaaaagcc
    13981 cgcgttccgg agggcctgaa gcgcggtctc cattggcgta gtagactccg ccatggcacg
    14041 gacacatgaa caattctgac tgtgggaacc agcggaccgg gcacccgaga tgtgcgcagt
    14101 tgatggcgaa tacctgaaac ttgtttccgc tcaggcggcg tacccagcac ggggtcttcg
    14161 ccgtgtcgcc gtcctgcgcg cgcttgaatg gattgatgaa ctcggcgagc cgcgtctgtc
    14221 cttcaggaaa ctgattgagg cttccgagag aaatccactg gttgtaagcg cccttgcgtt
    14281 tcaccggcga gaacagatat ccaatgactg gggtcgcaat ggcgatgccc acaaccgcgt
    14341 tcatcgcaat gccgatcttc atcagaagcg ttcgccgtgt tacctgcttt ccgtcactca
    14401 taacttcttt cctctggcgg tcccttcctg cgatggataa ggctgtccgg gtgtctgctg
    14461 gcgctggctt gccatccagg caaccaggtc tgtaacttcc tggtcgctca tggcgtggcc
    14521 gggctgcgca ttgcgccagt ccggatggcc gagatcgggc cgccccgcga tgatgatggc
    14581 gcgcagtgtc tggtcgctga taagcgcaag atacgagccg tcggtcacag aactggtctt
    14641 cgcattcagc ggctgcgctc cgctggagtg gcaacgcgag caatatgcct gaaagacctg
    14701 ctggccgcgg gccgcttcgc cctgcttgtc cggtttgtag ggagggacgt ctgcgccctg
    14761 cagatatccg gccttgaacc agcgctctct catgcctttc accagcgcgt ccacctgtgc
    14821 gtcggtgagc atgcctccgg cgctgcgggc aaaggcgggc atcagcgtcc ctggctcacc
    14881 gttggagatc acatcgcgca gagtattgtc gtccacaatc gcctgatact caggattggc
    14941 caaagcaatg gccgggccat gctggccgtc tgcgccgtga catgcagagc agttctgcgc
    15001 atacagacgc gaaaaatcgg tgacttcatc gggccgcagg gcctcagagc cgacgcgtgg
    15061 atttccggga aaccgctcgc atccggcagc cagcaataag ccgccaagca gtaaggcccc
    15121 gctaaggaac tggcgtacat tcatcggcgg tggtcctctc cttcttcggg gtgcagtccg
    15181 gatgcttcaa tacccatgcg aacgacaaaa ggcatggact gggtagtgcg aatacggctc
    15241 tggtgcgcga ccacccatcc ggcgaccagc ccgtaagtga tttgcgatgt cagaaaccat
    15301 ccccactgaa tgcgctcatc aaaggcagga ttgataatcc cgagcgttgc atgcagcagt
    15361 cctgtccaga gcagcggtgc gatgattcca cccaggatga tcggatgccg cggcaacatc
    15421 ggcagcatgg ctccatagag cagtccaacc agaatgctcg cggatgcgtg aataatgatg
    15481 gcaatgacca gcgccatggg atggaaggtg gcaatttccg ccgtggtggg gttcgtccag
    15541 tgcgctacgc ccgcgccacc cagcagattc accggatacc agatgctatg aaatccaatc
    15601 agcccgtaga gcagcgcggg aacgagcatg gcaaaaccgc ccgcaatgcc gcccttcagc
    15661 cctgagagca caggataggt ttccagcgga aggtgcgcgc ggtgctcctc tttgatttca
    15721 atacgagtga catgcgtgcg ctcggattcg atcgtgatct cgtgctcagt cacagggacg
    15781 tcttcatgcg cctcatgcgg cagcacctga cgaaaccatc ccacacaggc agtaacgaga
    15841 agtacagcgc caagaagact gacgaccaca tgtgtcacta gtccggcaaa gatcagagtg
    15901 gccccgaagg caagcacaat cggccacgca gtaggcgcgg gcagatggat cgtatctccg
    15961 gaatgaccgt gttgttcgtg cgattccata attctttaag gctggcagaa gcaccggttt
    16021 cagcccggcg aatcaaccca gcaggtatga ggggctttga gccccggtca accagtgacc
    16081 gctcaatcag cggccaatca cgtaaaccac cgtaaagacc acaacccaga ccgcatctac
    16141 aaagtgccag tacagggcca gcacctgaat gcgctcagcg tgctctttcc ggacgtggcc
    16201 tgtaatggtg aacaacagca ccgtcaaaag tgcgatcagg ccaataatca cgtgcgaagc
    16261 gtgcagtcca accagcgaat agaacgtagt accgaacagg ttggtgctga tggtcaggcc
    16321 gtcttcataa atcagcttgt gccactcgat gcctgtccca acaagaaaaa tcgcgcccag
    16381 tacaaacgtc agcgcccacc acaggccgaa cgccttgatt ttcccgtgct ctacggagcg
    16441 ctccgccagc cagatcgtca gactgctcga cagcaggaag atactgttcc atatcggcac
    16501 ttccaggatc tgcggggtcg gcccatagag gctcttgcca atgttgtaaa cataggccac
    16561 tacgaaaatc gtgaagatgg ccgattctgc gaggatgagg cagaacatgg ccacgcgccc
    16621 tttcgagggc tggatccacg gctgctctct ttctgcttgt gctactgcaa gtgtgctcat
    16681 cgcgttacgg cctcactcgt atctccagtc ggggtcttcc ggatgcttga tgtcccatag
    16741 cggacggcgg ctggtcacta ccggatcttc ggcaaagttg taggccggcg gcggagacgg
    16801 cgtcgcccat tccagcgtcc acgcgtccca cggatcgttg cctgctatcc tgcccttaaa
    16861 gtaggagtaa atcaggttaa aggcgaagac cagcacggca atggcctgga taaatccgcc
    16921 aatggaaacc acaaaattcc acgggcccca tccacggtct gcctcatagg taaagatcga
    16981 gcgcggcatg cccagaattc cagggatgtg catcgtgtca aaggtgacat ggaagccgat
    17041 tacaaacagc cagaagtgcc atttgcccag cttttcgctc aacatgcgtc cggtggcctt
    17101 gggataccag tagtagattc cggcaaaaat gcagaacaca atcgccccca ccagcacata
    17161 gtggaagtgc gcaatcacaa aataagagtt gtgcaactgc cagtcccacg gcgcaaccga
    17221 gagaatgatt cccgtcagcc ccgcaatcag aaactgaaag agaaacgccg tgcagaagag
    17281 catgggcgta gcaaagcgga ttttgccgcc ccacatcgtc gccacccagt taaagatttt
    17341 gattccggtc ggaataccca cgagcatggt ggagagcaca aagaacgtgt tgcccaccga
    17401 agtcattccg atggtgaaca tatggtgtgc ccacacccca aggctcacaa aagcaatgcc
    17461 gaccgaagcc gcggccatcg ccggataccc gaaaatggcc ttgcgcgaga aaaccggaat
    17521 cacttcattc gcaatggcaa acgcaggaag caccagcaca tacacttcag ggtgcccgaa
    17581 gatccagaag aaatgcgccc agatcagcgc cgatcctcca gcctgcgtat cgaagaaatg
    17641 tccgccaaga tagcggtcaa tcagcagcat cacctgcgct gcggtcagcg gagtaatcgc
    17701 aagcagcgcc agaaacgatg tcactacatt cagccagaca aacagcggca gccgcatcaa
    17761 cgtcatgcct ttgcagcgca tgcacaaggc cgtggcaatc acattaatgg aagcgccaat
    17821 ggtgccgaat ccggtaacaa tcagcgagag cgcccaatag tcgggtgcgt gcccgggaga
    17881 aaaagcgcgc tccgtcagcg gcgaataagc ccaccacatg gtgtccggcg cgctgcttgc
    17941 gccatagaga ccgtatccgc caataaagct gtaataaagc aggaggccgc cgaagaacgt
    18001 cagccagaaa ctcagagcat tcagcctggg aaatgccatg tcccgcgccc cgaccattaa
    18061 gggaatcagg taattggcaa acccaaagat gtaggtcatg cccacgagga agaccatcgt
    18121 ggtgccatgc atcgtgaaga cacggttaaa ggtccagggc gagagaaagt gcatgtccgg
    18181 acggatgagc tgaacgcgaa tcagaatcgc ctcgactcca ccaacaagca gaaagaagat
    18241 ggagtagagc atgtaaagaa tgcccagctt cttgtggtct accgtgacca cccagtcatg
    18301 cagccaggca agaaatgcgg cctgcgacca ccgccctctg tgcggggccg cctccaatgc
    18361 gcccggaacc ggaaatgttg agtctgccat aaatcccttt gcgcctttat cgcagcgtgg
    18421 acaggtatgc cgtcacctgg tcgagttgct catcgtttaa ttgcattgca ggcatcagcg
    18481 cgccaggctt gaacgtgtcc ggatttctga tccactcgcg cagatgatcg ggcgtatttg
    18541 ttgttgcgcc tgaggccaga gtttcgcggc tcatcagatg tgtcagatca ggcccgaagc
    18601 gccccgttgc cgccgtgcca gctatcgtgt ggcaattcat gcaggcctgt gtttcaaata
    18661 cgcgcttccc ttccgccact ttcggatcat tcaccgcctg ttgctgctga tttttcaccc
    18721 aggctgcaaa ttgctccggc gtgtccacat acacgcgcag cagcatcttc gcgtgctcta
    18781 caccgcagaa ctgggcgcac tgccctacat acactcccgg atggtgcgga tcaacccaca
    18841 agtaattgac tttgttcgga acgaggtccg ttttgcccga caattgcggc acccagaaac
    18901 tgtgaatcac atcggcagag agcagcgtaa gataagtcgg cgtaggatgg tccgggtcgc
    18961 tcaagggaac gtgcatctca ttgactgcgc gaatcccgag cttcgggtaa ttgaactccc
    19021 accaatattg atgtcccacg acagtcacat caagcgctga ttttggctta ggggcgtcct
    19081 ggatcgcgaa gatcatgcgc gccgtggtca ggaacaacac gacaacaatc aggatgggaa
    19141 caacagtcca ggcaagctca acctgcgtcg agccgtaaat ctgcgccggt tcgctggtgt
    19201 catcctgccc atgctggcga aagcgaaagg ccgcgtaagc aatcagcgag ccaacggtaa
    19261 caaaaattcc gccggtaatg gcgatgacaa acagcgagag ctgataaata ttggctgccg
    19321 gtgttgaagc aggcgcaaag atgctcggag tggattgcgg cccttcagca aataaagcct
    19381 gccctcccca gactataaac agaaacacaa accatttgac agaccacttc acgcatctat
    19441 aaggttctgt gagcatcgtt tcttaaactt ccttcaccgc gccagatggg gagcgaagac
    19501 aagatttgaa gctgataaag gacctgctcc ttcaaacaaa ggcgaaacct ctgcgaagaa
    19561 acaaagcggc cactcgcatt ccgcaagcag caggaatcat tacttttcta tcctaacagc
    19621 aatgaagctt ttctgctttg ctcagaaaca gaaattggtc catccccgaa cttttgctgt
    19681 atcagaaggt cctgctgtaa cacatacttc acatgttctg gatgcagcat gacttcagaa
    19741 gggcgcgatt ttcttgtctc cgctactgct ccgcaaccac agcgggcaga aaacggcgca
    19801 ctgtccctga gagtctgaac agattgaacc cttctgcata gctcttcagc actgcgctgc
    19861 ggacaaacgg catccgcatc gcgcgaaatc cgctctcggg ttttgcataa aaagatcttg
    19921 ctacacgccc tgcaatacga agatttccgc gtaggcccgc agtcagggat gcaaatgcct
    19981 cgcccgcatt tgcgttgcgg tccagtgcgg cgctgatggc cgaagccgcc gcctgcccgc
    20041 tcaccaccgc gccgtagatc ccttcgcctg tcagcggatc agcaaatccg cccgcatcac
    20101 ccacaagaaa aatacggctg ccggaaacaa cgtgccctgc tgcgccgaac ccaagatatt
    20161 gcccggcgaa gttttccgcc gccgcatccg tgccgtagcg cgttgcaata tattcagtta
    20221 acttcgcgcg gtccagtgtc tgcgcctgcc cccctgaata caacccgata ttcaaatgat
    20281 cgcgcttcgg gaagacccat ccatacccat ccaatacagg agcaaaatca aacaccagat
    20341 cgtcctttgc tccgtcacgc cgaaggttgg cctcgatagc aaatcctttg cggaaccagt
    20401 ccgtatcgcc ggtcagacgg cgcacctggc tgttggcccc atccgcacca accagaaaac
    20461 gtgcgcgcca ctcggcttct cgcgtaaaaa gggaaatacc actgacgttc tgcgcaatgc
    20521 gttctatcgc gccaatcctc tggaattccg cgcctccctc cagggtcttt ttaagacagt
    20581 aggcgtccag ctcttcgcgc acggtcatca cacagatgtt cttcggactg cgcaccagaa
    20641 tgtgtgagga gtcccgcccc tcaatcacaa ttctctggat ggtctcgcgc accaccggag
    20701 cgacagagta gcgcagggcg cgcagcgttt ttgccgtcag ccctccggca caggcttttt
    20761 tgcgcggaaa ctctgcttta tccagaagca gtacggattt tccccacgtt gcaaggtcgt
    20821 aggcacaggc gcatcccgcc gggccgcccc caacgacaat ggcatcccac agcttcatct
    20881 gtgttttaga ttattcgatg gccgtcatcg gcacacggat ggaagcacga tagcggtcac
    20941 cgcgatagcg tgagcaggcg gcctccaccg gcgtctcctg tgtcgtcata atcacgcgtg
    21001 taatcgagag aatgctcgcc ttgcgcggaa tagtcagcaa ctcggcctct tcctttgtgg
    21061 cggccagtgc ttcaatggtc tcatcggcat aacctacgcg cacgccgtaa cgctctctca
    21121 gggtctggta aagggagtgc tttgcaaaat caatgcggtc aagaccagga aattgcttca
    21181 gaggaatgtg cgagtcctca atggccatcg gaatgccgtc ggcaatgcgc agccggcgca
    21241 gatgcaggac cttgtcgttt ttgtccagtt tcaatttctg gaccaggtcc tctgtaggaa
    21301 tgatcacggc ctgttccagc aatctggagc tgggcttcat accgcgtttg cgcatctcct
    21361 cagtaaatcc ctggaggtgc atgatgtttt tttcaagctt gggccgcgta acaaaggtgc
    21421 cgcggccctt ctcactgaag gcatagccgc tggccttcag tccgtgcagc gcctgacgcg
    21481 ctgtcatgcg gctcacctga tagtgccgcg aaagctcttc ttctgaggcc agtgggtcgc
    21541 cttcggaaag ctctccggac tggatcttct ccattaacgc gcgctggatc tggtaataga
    21601 gcgggataaa accgttcttg tccaatggat gaaatgttgc ctggttttgt gtacggccct
    21661 ttgccacttt cgtccctcag tttcttacag attgccagaa tggcccaatc gtttaatatg
    21721 caaacattcc cgcagctcta tatgacacta accgccgcat ttcctgccag cttaaaaaat
    21781 aagctcatcg tttccgtaca ggccttcccc ggaggtccgc tggacgacac tgacacgctc
    21841 cgccgcatgg ccatcgccgc cgtgcgcgga ggagcaggcg gtctgcgcgc caatggcccg
    21901 gagcacatcg ccgctttccg gccggagacc tccctgccca ttatcggcat cctgaagcgc
    21961 ttcaatgaaa acggcccgct gattacgcct gatttcgcat ccgcaaaggc cgtggccgag
    22021 gctggaagcg atatcatcgg cgtggattgc agcgcacacc gccccgcttc cctggagccc
    22081 tggccggaac tgctcgcccg cattgccaaa gacctgcgca aacctgttct tgccgacatt
    22141 gcctctctcg atgaggccat tgctgccgaa aaagcggggg cgagcgccgt cgccaccacc
    22201 atgtatggat acactccgga aaccgagggc aagcgttcgg tggactggga gcttgtagag
    22261 cgcctggttg caggcctcag cattcctgtc attgtggaag gccacgtcca tacgccggat
    22321 gatgcccgcc gggcgctcga cctgggcgcc tttgccgtcg tggtgggcgc tgccatcacc
    22381 cagccggaat ggatcaccac aaggtttgtc aaagccatag aagagtagcc cgttacaccg
    22441 ttttagagaa agatttggcg gatacgtatc ttattctgcc cataaatttt cacctgtttg
    22501 tcattttttg cttgcttacc agtacagttg tctatacatt aagtgaaatc tggaaatcag
    22561 tctacaagat gcggctgttt ctttcagaaa tcgctcccct gccgcgtaaa cggacgcaag
    22621 ttccgtggcg gtattccatt cacatccctt cgtcggaggt ccacatccat gcagaaacgc
    22681 aaccctcatt ccctatggct cctgctgtgt actctgctgc tggtctgcat tcccagcacg
    22741 gcacagactg ttaccggctc catccgcggc acgattactg atccgggcgg agcaattgtg
    22801 gcaggcgcca aagtcattgc tacgaaccag gcgactggcg tcaataccga ggccgtcacg
    22861 aataatgccg gagaatactc catccgcttt ctccagattg gcagctataa gctgacggtc
    22921 gaggccgcgg gttttgagac cgccagctat ggcccgtttt ctctggagat tgaccaggta
    22981 gccaagattg atctggccct gaaggtcggc caggccagca ccaccgttac cgttaacgct
    23041 gaggcgcagc caattctaca gacggaaaat gccaccaatg gcgcaacctt tactgaaaac
    23101 accatcaaca gcgtcccact gaacggacgg gattttgcgc agattacagt atttactccc
    23161 gggaccattt cgacgggcta cggcaatttc ggcacatcga actcaaccga acggtccacc
    23221 accgacagca acgaacccaa tgtgaacggc agccgccagc agtcgaataa ctatctgctg
    23281 gacgggcagg agatcaacga gaacatcaac aatacgattg gctacaaccc tagtccggat
    23341 gcactcgaac aggtccgcgt catctcatcc aatgccaatg ctgagtttgg caatgtgaat
    23401 ggtggtgagg tggtgatggt gatgaagagc ggaagcaaca gcttccatgg cagcgctttc
    23461 ggctttctgc agaactacaa tctcgatgca aatagctgga agaacaaaaa caatgtggtc
    23521 ccggtgccga agaacccgta cacccagacc atcttcggag gaacgctcgg aggccccatc
    23581 atcaaggaca agctcttctt ctttgtggat tatcagggcg tccgggaaca taccggcgga
    23641 caatccacct tcagtcttgc gccgccggca tttctcaagg gcgatctttc ggccgtaaaa
    23701 aatgttctgg ggatccagct ttatgatacg cagcatctga ccaacggaca gcccactcca
    23761 tacccgaaca accaggtccc cgtgaacaac cctgtggcgc tgttcctcgc ggcccatcca
    23821 gaggcctatc ccgcccccaa taatccgacc agtgatcctc tggggatatt taacaactat
    23881 gtcgggccca gcaaaaactt tgtcagcaac gatcagggag atgtaaagat agactggcat
    23941 ctccataacg gagatgtgat ctcttcgcgg tactcacagg gatatgcgca gaacggaacg
    24001 gtcacggacc cggtacctgt gatcttcccc agcgcatcga attaccctga ccatctgggg
    24061 aatatcacct ggacccatag cttttctccg gctgtggtca attatgcccg cgcttcatat
    24121 gcgcgcatcc agttcaacag cggtgttacg accgatccca gcgggatctt cgggttcgat
    24181 ggcaatgcca aagtgggtat ccccagtaaa gcccagttga cacagggatt cagccagcag
    24241 tctttcagca ccaataacac caatggttat ccacagaact ttggcaccaa tcctacgccc
    24301 gaggtctttg tggacaatgt atttgaatat gccgataacc tcacctggca acggggccgg
    24361 catctgttca agttcggcgt tgaatttacc cgatatcagc aaaacagctt ctaccccggc
    24421 aatgatggtg aactgggtaa ctttaactac aacggccagt actcggccct tccgggatcc
    24481 accaatcttt atccgttcgc cgattttctc ctcgaccgtt cgaatgacgt cgctattgga
    24541 aacgtcaccg ggctgaccgg ccagcgccag tggcgtgacg gcatctttgc ccaggacgat
    24601 ttcaaggtga cacctacatt cacgctgaat ctgggcgtgc gctgggagta tgaccaaccc
    24661 atctacgaag tcaacaacaa gatggccaac atcaatatga caaccaaaca gattgagtat
    24721 gccggggtgg acggcaacag ccgagccctc tatgatccct attacgcaca ggtacagccg
    24781 cgctttggct ttgcctggca gccctctccg cgctgggtca ttcgtggtgg atatggcatc
    24841 accagctatc tggaaggcat gggagcaaac ctgcgcctga cccagaaccc gccattccat
    24901 accgactttg aagagcaggg cacggccccc agcgggtcag gcagctcgta caatcccggt
    24961 gtttactaca gtgcttcgga tggctttccg acgacgacac cgccgaccac aaccttctat
    25021 gtgtggccca aggacctgaa accttcctcc acccaggaga tcactcttac cacggaatat
    25081 gaagtcacac cgtcttcctc cctgcaggtg ggatatgtgg gaatcctggg gcaccatctc
    25141 acgaatcctt actggggcaa tcagttgcct gctccgggtg caactgcgcc ttatgcgaat
    25201 atcgtgggac agagcggtgt gatcaaaatc agcgccacca atgcggccag caattacaat
    25261 gcgctgcagg ccgtctaccg gcagcgcctg acggctggtc tggagctgac agcaaactac
    25321 acctatgcaa agtctctgac tgatagcttt ggctactacg gtgtctctaa catcagcggg
    25381 caatattacc aacagaacgc ctatgatatg gcggcggagt gggggccagc gggattcgat
    25441 atccgtcatg ccctgagcgt ttccggcgaa tacgacctgc ccttcgggcg cgggagacag
    25501 ttcggcggag gcatgaaccg cctgctcgat gcagtcatcg gaggctggaa gctggcaggc
    25561 accaatgtga cctatacggc cttccctgtc acagcaggct cgccggccaa ctactccagc
    25621 ctggtgcatg cagctacggc caggccgaac cagtatcgtc cgctgaagat cgtcaatcgc
    25681 tcgctcaacg catactttgg cacggcggtc cagggaacgg cctgcggagc agccccaaat
    25741 ctcggcccca atgccggcac ctgcatcttc ggcgcgcagc ccacagacac ctttggcaat
    25801 gttcgtccca tgtccctgcg tggacccgga tatgagcaga ttgatatgtc ggtgtccaag
    25861 tccttcaccc tgtggaaaga gcacacgctc aatttccgca gcgacttctt caacgcattt
    25921 aacattgcca gttacagcgc gccggacaac aacgttaccg acgcatcgtt tggccagatt
    25981 accaatacga cctcgactga gcgccacatt cagtttggtc tcaagtactc cttctgaaca
    26041 gtctggcctt cccagggcgt ggagctttcc acgccctttc tttttcgcaa aaatttagcg
    26101 gtatagctgt ccatacaggt ttgatttaag cgaaaataaa accatgtccc gttcgtttcc
    26161 tcatgcgatg ctgcgcgaaa tttacgagca gcctgcggcc ctttcttcca ccattcagaa
    26221 ttacgtctca gaccaggcgc tcaagcccga agcattcgcc gccgtcgccg atgcgctgcg
    26281 cggacaccag cgcgtggtca tcgcggccag tggttcaagc cgccacgccg ggcttgcagg
    26341 cgagatcatg ctggaagacc tggccgggct gaccgtagac gtggaatacg ccagcgaata
    26401 ctgctatcgc tcgactcata cccttcagga ccccggcgtc atcgttattt cgcagtcggg
    26461 cgagaccgcg gacacgctcg ccgccctgcg tgaaggaaaa tcgcgcggcc tctcgaccgt
    26521 ggccatcacc aacaatccca actccaccat ggcacgcgag gccgatgctt ccctgcccac
    26581 catcgccggc aaagaaaagg ccattcccgc aaccaagagc ttcaccacac agcttgccgt
    26641 gctgtatttg ctgacacttt ccatggcgcg cctgcgcgga cgcatgacgg cccactcagt
    26701 ctctgcgctg attgagcagc tggaagcctt gccgggactg ctcgaacgct cactccccgc
    26761 atgggaagag caactgcgtg ctgtcgcaga acacaccagg aatgcgcgcg gctttctgta
    26821 tctgggtcgc ggcattcatt acgccatcgc gcgcgaaggc gccctcaagc tgaaggaatc
    26881 tgcctatatt caggccgaag gatatcctgc gggcgagctg aagcacggcc ccaatgccct
    26941 ggtcacgaaa gatgccccgc tcgtgatgct ggccacgcag gaccgcaatg atcccgattc
    27001 cgttctgcgt tatgaaaaga ccctgcagct catgaaggac atgcaggcgc aaggcgcaga
    27061 gatccttgcg cttgcttcag aaggcgacag cgaagtttcc cgggtggcgc gctttaccat
    27121 caccgttccg aacgccagcg aatatcttct gaccatcctc gaagtcgtcc cactgcaact
    27181 cttcgcttat ttcacagcca tcctgcatgg agtagacgta gacagcccgc gcaatctggt
    27241 caaggccgtc gtccaggaat aaagaaaggc ccacgctcct gatgactctg agagcgcggg
    27301 tcttttttgc tttttcgcta tagcattttc acagatagtt tggaccatgc tgcgaaacta
    27361 cagctgcctc ggccaacagg gaggcggcgg cactgcacag tgcaaatgga gtctccagaa
    27421 tctgtggaaa tgccttagtc tgggtgctcc gcgaaaaatc tgaacatgct ccctgacatg
    27481 gcactggctg gcgaaaatcg tgcccggcat cactcggcct tgatatcctt caggcagtga
    27541 agctgaaaga tctccctttt tccatttctg tatccggcgc agttgttgcg gccctccttg
    27601 tggctggctg tgcgcggcaa ccatccgcag caaacgatgt tcaagcggcc aggcaggcac
    27661 agcagcaggc acgcgctgct gagaaggcgc agatggacgc cgcgcgtgaa gcgctggacg
    27721 ccattcccct gccggcaaaa agccgttaca tggccgtccg caacacggag agctggagca
    27781 atccttttct catcgtaggc agcgaaaatg tgaccctgcg ggtcgtttat cctgacgtga
    27841 cccacaacaa cgctctgccc agcaccatgc tgaagcccgc aaaggcgcgc cgtcaggaac
    27901 tgacaatccg gctctctgat cttccggatg ccttaagctc tttgccaaac gacgaatggc
    27961 cctatggccg cgtggttgcc gtagaagaag acccgcttga aacccgcgcc aatcgccttc
    28021 aggtacgcag gaacatcgaa actaccatgc aggtgctcaa taacctgggc gtcgttgtct
    28081 atgaa
//
LOCUS       DASRBV010000319         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_692884, whole genome shotgun sequence.
ACCESSION   DASRBV010000319 DASRBV010000000
VERSION     DASRBV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692884"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      98..212
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            381..1652
                     /gene="thrC"
                     /locus_tag="VFA02_11880"
     CDS             381..1652
                     /gene="thrC"
                     /locus_tag="VFA02_11880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZU10592.1"
                     /translation="MASSYELCCRECGRKYANAPLSICDECFSPLEVQVDLEAAKKTV
                     TRETIAQGPTNMWRYQALLPVPENYVPQTPAGWTPLLKAPRLAERIGANNLYIKNDAV
                     CLPTLSFKDRVVAVALAAAQDFGFDTVGCSSTGNLANAVAAQAARLGLKTYILVPADL
                     EAAKILNTQVYGANLIRIDGNYDHVNRLCSQIADRFHWGFVNVNLRPYYAEGSKTVGY
                     EIAEQLGWRLPDNVVVPMAGGSLITKIRKAFRELVVLGLVEDKPVRFFGAQATGCSPI
                     AKAVKSGADHIEPQRPSTIARSLAIGNPADGVYAARAIRESGGWAEDVSDVEIVSAIQ
                     ELAETEGIFTETAGGVTTAVTARLYAHGRIKPGELTVSCITGNGLKTTDVLQGAYEQG
                     QAIRPRLTDFENYINEIKELNELVTGGVNVR"
     gene            1642..2022
                     /locus_tag="VFA02_11885"
     CDS             1642..2022
                     /locus_tag="VFA02_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZU10593.1"
                     /translation="MSVRVLLPNAFQKHTNGAKEIQSTAANLPALIEDIETHFPALKT
                     HLRDETGNVRRFINFYVNEEDIRFLGNEKYAFQDGDEVLVIPSIAGGSCGEDDTRLAQ
                     NVTSSRRPVLRCCSYCGQEECICY"
     gene            2125..>3350
                     /locus_tag="VFA02_11890"
     CDS             2125..>3350
                     /locus_tag="VFA02_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZU10594.1"
                     /translation="MPPSLSKFVLCAFAFPCFAWAQQTSSPFYQHPDAPIEQRVSDLL
                     ARMTVEEKVRQLDLYFGAQDLVDKRADKTHAAPDAVFLPEKAEQLWGNLGVGGIHDLY
                     PTPQQSNAIQKWVIGHNRLGIPAIFIEEGLHGFSDGTVFPAPINLAATWDPALARKTG
                     AAIAAEARATGVDMILAPVLDLAREPRWGRIEEDFGEDPYLTGQMGLAYVRGAQGDSL
                     ASDHTVITEPKHFAAHGSPEGGSNTSPVHIGERELRTVMLKSFEPAIREGHAMGVMAA
                     YHEIDGIPVTADPFLLKKILREEWGFQGFVLSDLGAIQRLYNAHYVAATPKDAACLAI
                     KSGVDMQFYDFPHDVFQKALIDCVREGTLSMADLDRAAGSVLRVKFALGLFDHPFVDT
                     NLNAKVHRSPEHLSVSL"
BASE COUNT          719 a         1005 c          941 g          685 t
ORIGIN      
        1 acctcggcac ccaggcttct tttctccctg cttgcattct cccagcccct gtggtacaaa
       61 cgaatcattc aagtttgctc cttgtgggcg agcctgaatt tcatgcagag tggctgaggg
      121 acgggccctg tgacgccacg acaaccggcc tgagcaattc aggacagcgg tgtcaactct
      181 cgctcacctc aaaagggtgg gatacatgag attcggggtg caggctgtat cgcatctcgc
      241 taaatcccag gtgttggcag tactgaagta aaagtagtag gcagtaccag agttaccgtc
      301 acttgcccgt gcataactgc accttgccgg agcgcatccc gtcgaggccc accacgccgc
      361 tctcgattgc gagggcaaag atggcttcat cttacgagtt atgttgtcgg gagtgcggca
      421 ggaaatacgc aaacgcgccg ctctccatct gcgacgagtg tttctcgccg ctggaagtac
      481 aggttgatct ggaagccgca aagaagacgg tgacccgcga gacgattgcg cagggtccca
      541 cgaatatgtg gcgctaccag gcattgctgc cggtcccgga aaattatgtc ccgcagacgc
      601 ccgcgggatg gacgcctctg ctgaaggccc cgcgtctggc cgagcgaatt ggcgcaaaca
      661 acctctacat caagaacgac gctgtatgcc tgcccacatt gagctttaaa gaccgtgtgg
      721 tggcggtggc ccttgcagcc gcgcaggact ttggctttga caccgtaggc tgctcctcga
      781 ccggcaatct cgccaacgca gtggccgcgc aggcagcccg cctcggcctg aagacctaca
      841 ttctggttcc cgctgatctg gaagcggcga agattctcaa cacgcaggtc tatggcgcaa
      901 acctcatccg cattgacggc aactatgacc atgtgaaccg cctctgctcg cagatcgctg
      961 accgcttcca ctggggattc gtgaacgtca accttcgccc ctattacgcg gaaggctcga
     1021 agacggtcgg gtacgaaatt gccgaacagc tcggctggcg cttgccggac aatgtcgtcg
     1081 tcccgatggc tggcggctcg ctcatcacca agattcgcaa agcattcagg gaactcgtcg
     1141 ttctcggcct cgtggaagac aaacccgtgc gcttctttgg agcgcaggcc acaggttgct
     1201 cgcccatcgc aaaggccgtc aaatccggcg ccgaccacat tgagccgcag cggccctcga
     1261 cgattgcgcg ctctctggcc atcggcaacc cggccgatgg tgtatacgcg gcgcgcgcca
     1321 tccgcgaaag cggcggatgg gccgaggacg tttcagacgt cgagattgtt tcagcgatcc
     1381 aggagctggc ggagaccgaa ggcatcttta ccgaaaccgc aggcggcgta acgacggctg
     1441 tgacggcgcg gctgtatgcg catggacgta ttaagcccgg cgagctgaca gtaagctgca
     1501 tcaccggaaa tggcctgaag acgaccgatg ttctgcaagg cgcgtatgag cagggtcagg
     1561 cgattcgtcc gcggctgacc gattttgaga actacatcaa cgaaatcaag gaactgaacg
     1621 agctggtaac aggaggcgta aatgtccgtt agagttttgc tgcccaatgc atttcagaag
     1681 cacaccaatg gcgcaaaaga gatccaatcc accgctgcaa acctgcccgc gctgattgaa
     1741 gacatcgaaa cgcacttccc tgctcttaaa acgcaccttc gcgacgagac aggaaatgtc
     1801 cgccgcttca ttaacttcta cgtcaatgaa gaagacatcc gcttcctggg caacgagaag
     1861 tacgcattcc aggatggcga cgaggtgctg gttattccct ccatcgccgg cggctcctgc
     1921 ggcgaagacg acacgcgcct ggcgcaaaac gtcacctcct cgcggcgtcc agtgctgcgc
     1981 tgctgctcct attgcgggca ggaagagtgt atctgctatt aaagccactt cgcatatgcc
     2041 tcactcattc cgggtggggc atttttgttt ctggttgcag atggcgggac cggcatggta
     2101 tcgtttccac ttcccggaga aaccatgcct ccctcactct ctaaatttgt tctttgcgcc
     2161 ttcgcctttc cctgcttcgc ctgggcccag caaacttcat ctccgttcta tcaacatcct
     2221 gatgctccca tcgagcagcg cgtcagcgac ctgctcgcta gaatgaccgt ggaagaaaaa
     2281 gtccgccagc tcgatctgta tttcggagcg caggacttag ttgataagcg cgcagacaaa
     2341 acccatgccg caccagacgc agtttttctc ccggaaaaag cagaacagct ctggggaaat
     2401 ctgggcgtgg gcggcattca tgacctctat cccacaccgc agcaatcgaa cgccattcag
     2461 aaatgggtaa tagggcataa ccgcttggga attccggcca tctttatcga agaaggactg
     2521 cacggcttca gcgatgggac ggtcttccct gctcccatca atctggccgc aacatgggac
     2581 cccgcactgg cccggaagac aggcgcagcc atcgccgctg aagcacgcgc caccggcgtg
     2641 gacatgattc ttgctccggt gctcgacctg gcgcgcgagc cacgctgggg tcgcatcgaa
     2701 gaggacttcg gcgaagaccc gtatctcacc ggccagatgg gtcttgccta tgtgcgcggc
     2761 gcacagggag acagtctcgc ctcagaccat accgtcatca ccgagccaaa gcactttgcc
     2821 gcgcacggct caccggaagg cggctcgaat acttcgcccg tccacattgg cgagcgcgag
     2881 ttgcgcaccg tcatgttgaa atcgtttgag ccagccattc gcgaagggca cgcgatggga
     2941 gtgatggccg cttatcacga aatagatggc atcccggtca ccgccgatcc atttcttttg
     3001 aagaaaattc tgcgcgagga atggggcttt cagggatttg tgctttccga cctgggcgcg
     3061 atccaacggc tctacaacgc gcactatgtt gcagcaacgc cgaaggacgc tgcatgtctc
     3121 gccatcaagt cgggcgtaga catgcagttt tacgactttc cgcacgatgt ctttcagaaa
     3181 gcgctgatag actgcgtgcg tgaaggcacg ctctcgatgg cagacctgga ccgcgccgcc
     3241 ggttctgtgc tgcgcgtcaa atttgcgctg gggctcttcg accatccatt tgttgacacg
     3301 aacctgaatg cgaaggtgca tcgctcgccg gaacatctgt cagtttcgct
//
LOCUS       DASRBV010000320         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_124696, whole genome shotgun sequence.
ACCESSION   DASRBV010000320 DASRBV010000000
VERSION     DASRBV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124696"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1148)
                     /gene="menC"
                     /locus_tag="VFA02_11895"
     CDS             complement(27..1148)
                     /gene="menC"
                     /locus_tag="VFA02_11895"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZU10595.1"
                     /translation="MKIDSIVLREIRMPLVQPFQTSFGTTTDRRIMLVELRAEGLTGW
                     GECVAGEHPYFSEETIDTAWLVIMQELAPALASADPEHGGKCPGIFRQVRGHRMAKAA
                     LENAVWDLEAQMRGVPLAELLGGTREVIPCGVSIGIQPTMEKQLAEVEKELAAGYQRI
                     KLKCKPGWDVEMFEAVRNRWPDILLSCDANSAYRMNDMDHLASFDAFDLLMIEQPLWS
                     DDFYFHSMLQKRIRTAICLDESIRNRRDALAAIDMGSCRIINIKNGRVGGFSEAVAVH
                     NAAQEKGVPVWCGGMLESGIGRSHNIALSSLENFSLPGDVSASKRYWKEDIIEPEVEV
                     SPQGEIRVPDSPGRGFAVRTDLIEKLTVRKEEVRALALA"
     gene            complement(1156..2019)
                     /locus_tag="VFA02_11900"
     CDS             complement(1156..2019)
                     /locus_tag="VFA02_11900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10596.1"
                     /translation="MPSTNSTTFTTPQGREILIRHCSGFEELDACVQLQIDVWGYSDG
                     DVIPRRVFTVTQKIGGQVLGAFDVTGDRGDVSEHLVGFAMALPAVRENPPPNAGEGLN
                     GAPDAGLEAYLHSHMLAVNPEYRNSGIGRRLKLAQREDAVARGFRLMEWTFDPLEIKN
                     AFLNIHRLGAIVRRYTPNFYGVSSSRLQGGLPTDRLHAEWWMQSERVKAVLAGKATEP
                     VRVEQTITVPREIYEWKSSAADHTRALEVQTGNRQLFLDAFARGLAVIGFRTDAKGNG
                     TYELGAWQEPK"
     gene            complement(2041..>2799)
                     /gene="mazG"
                     /locus_tag="VFA02_11905"
     CDS             complement(2041..>2799)
                     /gene="mazG"
                     /locus_tag="VFA02_11905"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZU10597.1"
                     /translation="PWDREQTFDSIRKYTLEETYEVLDTIERRDWSHLKEELGDLLLQ
                     VLFYAEMASEEGHFRISDVISELNRKLIRRHPHVFGDEASAQAGNIAEGLETNGIDAK
                     QVLRNWEEIKHREKSAGEEKRGRLDGIPRSLPALMEAQKLGSKAAKAGFDWPDTRGLF
                     EKLQEEIQELEAELAQAEQNHDAISLEMGDLLFTAVNLARHLKVDPEMALRETNAKFR
                     RRFAQMEAASQRPLEELPQEELEHLWNKAKRLER"
BASE COUNT          552 a          855 c          738 g          654 t
ORIGIN      
        1 ccggaaccgc tctgaggcgt cctgtgtcac gcaagcgcca gtgcccgtac ctcttcctta
       61 cgcacggtca gtttttcgat caggtcagtc cttacggcaa acccgcgtcc cggagaatcc
      121 ggcacgcgaa tttcgccctg cggcgatact tccacctccg gctcaatgat gtcttccttc
      181 cagtaacgct tcgatgcgga gacatccccc ggcagggaaa agttttcgag tgatgaaagc
      241 gcgatgttat gcgagcgtcc aatgccgctc tcgagcattc ccccgcacca tacaggaacg
      301 cctttttcct gcgcggcgtt gtgcacggca accgcttcag agaagccgcc gacgcggccg
      361 ttcttaatgt tgatgatgcg gcacgagccc atatcaatcg cagcaagagc gtcgcggcgg
      421 ttgcggatag attcgtccag acagatggct gtccggatgc gcttctgaag catggagtgg
      481 aaatagaagt cgtctgacca cagcggctgc tcgatcatca gaaggtcaaa ggcatcgaag
      541 ctggcaaggt ggtccatgtc attcatgcga tatgccgagt tggcgtcgca actcagcaga
      601 atgtctggcc agcggttccg caccgcttca aacatttcca cgtcccatcc gggtttgcac
      661 ttgagcttga tacgttggta gccggctgcc agctctttct caacttcggc aagctgtttt
      721 tccatggtcg gttgaatgcc gatggaaacc ccgcatggga ttacctcgcg cgtgccgccc
      781 agcagttcag ctaacgggac gccgcgcatc tgcgcttcca ggtcccacac ggcattttcg
      841 agcgcggctt tggccatgcg gtgtccccgc acctggcgga agatgccggg gcatttgcct
      901 ccatgttcgg ggtcagcgct ggcaagggcg ggcgcaagct cctgcatgat aacaagccag
      961 gctgtgtcta tggtttcttc actgaagtaa ggatgctcac cggcaacaca ttcgccccat
     1021 ccggtcagcc cctcggcgcg gagttccaca agcataatgc gccggtcggt ggtcgttcca
     1081 aagctggtct ggaacggctg caccagcggc attcgtattt cccgcagaac gatggaatca
     1141 atcttcatac aaatctcact tcggttcctg ccaggcgccg agttcgtagg tcccgttgcc
     1201 tttggcatct gttctaaagc caatcacggc cagcccccgt gcaaacgcat cgagaaaaag
     1261 ctggcggttt ccagtttgca cttcgagtgc ccgtgtgtgg tctgctgctg acgatttcca
     1321 ctcgtaaatc tcgcgcggga ccgtaatggt ttgctcaacc cttactggtt ccgtggcctt
     1381 tcccgcaagg actgccttaa cgcgctccga ttgcatccac cattccgcgt gcagacggtc
     1441 ggtcggcagc cctccctgga gtcgagatga ggatacacca taaaagttag gcgtatacct
     1501 gcggacaatc gcccccagcc tgtggatatt cagaaaagcg ttctttatct ccaggggatc
     1561 aaaggtccat tccataaggc ggaagccgcg ggcaaccgcg tcctccctct gggccagctt
     1621 cagcctgcgc ccgatgccgg agttccggta ttccggattt accgccagca tatgcgaatg
     1681 cagataggct tccagccccg catccggtgc cccattcaaa ccttctcccg cattgggtgg
     1741 gggattctcc cgcaccgccg gaagcgccat cgcaaaccca accaggtgtt cggaaacgtc
     1801 gcctctgtcg ccggttacat cgaacgctcc caacacctgc cctccaattt tctgagtgac
     1861 ggtaaatacg cggcgcggaa tgacgtcgcc atctgaatat ccccagacat caatctgcaa
     1921 ctggacacag gcgtccagtt cctcgaatcc ggaacagtga cgaatcagaa tttcccgtcc
     1981 ctgcggagta gtaaaggttg tgctgttcgt actcggcatt cttttctctg cggtaaggac
     2041 ctatcgttcc agcctttttg ccttgttcca aagatgttcc agttcttctt gaggaagctc
     2101 ttccagcggc ctttgactgg ctgcttccat ttgagcaaaa cggcggcgga atttggcatt
     2161 ggtttcgcgc agggccatct ctgggtctac cttcaggtgg cgcgccaggt tgactgccgt
     2221 aaacaggaga tcgcccatct caagagaaat cgcgtcgtgg ttttgttcag cctgcgccag
     2281 ttccgcttcc agctcctgaa tctcttcctg tagcttctcg aaaagaccgc gtgtgtccgg
     2341 ccagtcgaag ccggccttcg cggcctttga tcccagcttc tgcgcttcca tcaatgccgg
     2401 aagggaacgc ggaatcccgt caagcctccc ccgtttctct tctccggcag atttttccct
     2461 atgcttgatc tcttcccagt tgcgcagcac ctgctttgcg tcaatgccat tggtttcgag
     2521 gccctcggca atattgcctg cctgtgctga agcttcatcg ccaaaaacat gcggatggcg
     2581 gcggatgagt tttcgattca gttcggaaat gacatcggaa atgcggaaat gcccctcttc
     2641 cgaggccatt tccgcataaa aaagcacctg cagaagcagg tcgcccagct cttctttcaa
     2701 atgggaccaa tcgcgccgct caatcgtatc caggacttca taggtctcct ccagcgtgta
     2761 tttgcggata gagtcaaagg tctgctcgcg gtcccacgg
//
LOCUS       DASRBV010000321         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_171818, whole genome shotgun sequence.
ACCESSION   DASRBV010000321 DASRBV010000000
VERSION     DASRBV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171818"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFA02_11910"
     CDS             complement(<1..236)
                     /locus_tag="VFA02_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10598.1"
                     /translation="MPRTTSKVFWNAMSNRPVFNRLIGWCFHNFLQRGPAERKYWASF
                     AQRRGKQLSVELTEAAKQEQIIHALLLPPVFIQL"
     gene            560..2008
                     /locus_tag="VFA02_11915"
     CDS             560..2008
                     /locus_tag="VFA02_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZU10599.1"
                     /translation="MAGIETTLGDGPQRSGRVSGMPGDLVTAVISPDGTPNGQPNGVA
                     PGIVPQPLPLQIDGGGTPADAKDSKNAGSSAPVQREQVEAPPSQMAALISDGMVPPDF
                     ERPVAVSVSKDQRALQTESGGEGIYEENGMTPTTDGEVSQHAEQLQGHATELQKMLPG
                     IENQQFGPVSAVMKGNAVPLPAEPPDANPCKVHAQAGDHAVGASNHQKQETGGSRDHG
                     SHSEGSRICAEQERGTGFAIDKAESGGPCDPANDRKNGQIPASRRTDSAAPQGPAAGT
                     TPDNYVSAAVTSTAAASAQFAGQGQAQSSPAGAAPPGACLLRQPVPAHAATAGTLTAA
                     AESPAEHSGAVVSAATMLQSYGKAEMRVTMHTQSLGPLELHAVLDGGRLGASISVVNH
                     EAHTLLSNSLASLQQSLIDRNLRVDHLSVLNASAGGGTNTGNSGGCDSRGQAHPRPNA
                     PAWIFSRPAALRGTGRESAAAEVVRGRLSVHA"
     gene            2035..2346
                     /locus_tag="VFA02_11920"
     CDS             2035..2346
                     /locus_tag="VFA02_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZU10600.1"
                     /translation="MMTSFNPMTTSQEAASMAGASSAGQSSAPGIQAKQITTQDFMTL
                     LTAQLQNQDPTNPVDPTEFVTQLAQFTTLDEVSQINSLLQTLVQSAMAPTGTQAASGA
                     A"
BASE COUNT          578 a          721 c          748 g          420 t
ORIGIN      
        1 tcgagttgga tgaaaacagg cgggagcagc agcgcatgga tgatctgttc ctgcttcgcc
       61 gcttccgtca attccacgga tagttgtttc ccccgccgct gggcaaagct tgcccagtat
      121 tttctttctg caggtccacg ctgcaggaaa ttgtgaaaac accaaccgat taacctgttg
      181 aacacaggac ggttgctcat cgcattccaa aacactttcg atgtggtccg tggcatgcat
      241 cggcgtgtgt gcgaaaggcc gtcaggcgat gattgcagga gtacaagaaa cagtaaacgg
      301 tgtgcccgcg ctccccgtac aggagcattc tgccgcacac ggaggagcag gctttaaggg
      361 cttgctggat tccgtaatgc agaaggccgc ccctccggcg caactgggtt tggcacagct
      421 tggggagact cgttcttccg acaaaccgga gcaacaccga aacactctcc aatctgggac
      481 gaatttacaa acgcctgccg taggtgccgt aatgaccggc gctcccagcg cgcccgttcc
      541 cgccgcgaat caaaggcaca tggctggaat tgaaacgaca ctgggcgacg gcccgcaacg
      601 ctccggccgc gtatcgggga tgcctggcga tttggtgacc gccgtgattt cgccagacgg
      661 aacaccgaac gggcaaccga atggagttgc acccggcatc gtgccgcagc ctctccctct
      721 gcagattgac ggcggcggca ccccggcgga cgcgaaggat tccaagaatg cgggttcgtc
      781 ggctccagtg cagcgagaac aagtggaagc tccgccttcg cagatggccg cgctgatttc
      841 ggacgggatg gttccgccag attttgagcg gcctgtagcc gtgagcgtca gtaaagatca
      901 acgggcgctc cagaccgaat ccggcggaga aggcatttat gaagaaaacg ggatgacgcc
      961 gacaacagac ggcgaggtgt cgcaacacgc cgaacagctt caggggcatg cgacagaact
     1021 gcagaagatg ctgcccggca ttgagaacca acagtttggc ccagtctccg ccgtcatgaa
     1081 aggaaatgcc gtgccattgc ctgcagagcc acctgacgca aacccctgta aagtccacgc
     1141 gcaagccgga gatcatgcag tgggcgcgtc aaatcatcaa aagcaggaga cgggcgggtc
     1201 ccgagatcac ggcagtcaca gcgaagggtc ccgcatctgc gcagagcagg aaagaggaac
     1261 aggttttgcg atcgataaag cggagtcagg tgggccgtgc gacccggcaa acgacaggaa
     1321 aaatggacaa atccctgcct caaggcgaac cgactctgcg gctcctcaag gtcccgcagc
     1381 gggcaccact ccagacaact acgttagcgc ggcggtcaca agcacggctg cggcatcagc
     1441 gcaatttgct ggccaaggcc aggcgcagag ttcgcccgca ggcgcagcgc ctccgggcgc
     1501 gtgcttgttg cgtcagccgg tccctgcgca cgctgcgacg gcggggacac tgacagcagc
     1561 cgcagaaagt ccggcggagc actccggggc ggtggtcagc gcggcaacca tgctccagtc
     1621 gtatggaaaa gccgagatgc gggtcacaat gcacacccaa agtctgggtc cactggaact
     1681 ccatgcagtg ttggatggag gccgtctggg agcatcgatt tctgtggtta atcacgaagc
     1741 acacacgctg ctgagcaaca gtcttgcatc gctccagcag tccctcattg accgaaacct
     1801 tcgagttgac cacctttcag tgcttaacgc atcggccggg ggcggtacaa atacggggaa
     1861 cagcggaggg tgcgactcca gaggacaggc gcacccgaga cccaacgctc cggcatggat
     1921 tttctcccgg cctgcggcgc tccgcggtac cggcagggaa agcgcagcgg ccgaggtcgt
     1981 gcgaggaagg ctcagcgtgc acgcataaat tctggataga agaggagaat tgcgatgatg
     2041 acgtctttta atccgatgac aaccagccag gaggcggctt cgatggccgg cgcaagttcc
     2101 gcggggcaat catcagcgcc gggcatccag gcgaagcaga tcacgaccca ggattttatg
     2161 acgctgctca cggcgcagct tcagaaccag gacccgacaa accctgttga cccgacggaa
     2221 ttcgtaacgc aattggcgca gttcacaaca cttgacgagg taagccagat caacagcttg
     2281 cttcaaaccc ttgtccagag cgcaatggcg cccaccggaa cgcaagcggc ttctggagcg
     2341 gcgtgaattg ggcccacatg gacagtgtca ctctctgcgc ggcggcgagt gaacgagcaa
     2401 aacaagattg aagtgttcga ttcccagaat tcaggagaac aaccatgcca tcattttcga
     2461 tcccggt
//
LOCUS       DASRBV010000322         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_276404, whole genome shotgun sequence.
ACCESSION   DASRBV010000322 DASRBV010000000
VERSION     DASRBV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276404"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1518
                     /locus_tag="VFA02_11925"
     CDS             97..1518
                     /locus_tag="VFA02_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU10601.1"
                     /translation="MDRGFLLGGRECFDGEEVVVRSPWDGSVVGRVRQARRGEALEAV
                     CMLEKSFAVTRKLPAHERKHILLEVAAKIAAQKEELARLIVAEAGKPLKAARVEVERA
                     VLTFTTAAEEAARMGGEVLPLDLLKGSEGRWGMVRRFPAGPVFAITPFNFPFNLVAHK
                     LAPAVAVGCPVLLKPAPQTPFCALKLADFLLEAGWPADALAVMPMANEDAEHLLANDD
                     RLKVLSFTGSAKVGWALKQKAGKKRVLLELGGNAAMVVHSDWADLEGAAKKAVLAGTS
                     YAGQSCISVQRVYVERPVFDEFVQHAMRHTGLLVLGDPANEETDVGPVIRESDAERIE
                     SWIDEARAAGTTVLTGGSREGSLVEPTLLTGTRRGMKVLDEEVFAPVLAIEPYDDFGA
                     VLDEVNRSPYGLQAGLFTRDVGRIFRAYEALDVGGLIVGDTPTWRLDPMPYGGVKDSG
                     LGREGLRYTMEEMTEPKLLVMAL"
     gene            1617..>2231
                     /locus_tag="VFA02_11930"
     CDS             1617..>2231
                     /locus_tag="VFA02_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU10602.1"
                     /translation="MSNKLMQIEPQGPATELIYNDWYPAMRSDGLHGKTLKTTLLMGI
                     PMALGRRADGRIFAMRDLCPHRGIPLSYGWFDGQKLTCKYHGWSFEPCSGQCVEIPSL
                     TSRDTLDPKRIYGTAFPCEERDGYAWVYVPEPGSGRVRESDELPPVPELPKFSAKFRS
                     QHLTAELPCNVDHGIIGLMDPAHGPFVHQSWWWRKQASIHEKEKH"
BASE COUNT          461 a          538 c          784 g          448 t
ORIGIN      
        1 cggcatcgta tcgtacaggg cggcgtccgt cagtcattct gaaggagagt gtacttgaaa
       61 agagaacaaa tggcgaagag cactactgct ggtgctatgg atagaggctt tttgctgggt
      121 ggacgggagt gctttgacgg cgaagaggtt gtggtccgtt ctccatggga cggaagcgtt
      181 gttggcaggg tacggcaggc gcggcgggga gaggcccttg aggccgtgtg catgctggag
      241 aagtcgtttg ccgtaacgcg gaagctgccc gcgcacgaac gtaagcacat tctgctggaa
      301 gtcgctgcga aaattgccgc gcagaaagag gaattggcac ggctgattgt ggcggaagca
      361 ggcaagccgc tcaaagctgc acgagtcgaa gtcgagcggg ccgtgttgac ctttaccaca
      421 gcagcagaag aggccgcgcg tatgggcggc gaggtgttgc cgcttgatct tctgaaagga
      481 agcgaaggcc ggtggggaat ggtgcggcgt tttccggcgg gcccggtgtt tgcaattacg
      541 ccgtttaatt ttcccttcaa tttggtggcg cacaagctcg ctcccgcggt ggcggtgggt
      601 tgtccggtgc tgctgaagcc cgctccacag acgccgttct gcgcactgaa gctggcagat
      661 tttctgctgg aggctggctg gccggcagat gcgctggcag tcatgccaat ggccaacgaa
      721 gatgctgagc atctgctggc aaatgatgac cggctgaagg tgctgagctt taccggaagt
      781 gcaaaagtgg gctgggcgct taagcagaaa gcaggaaaga agcgcgtgct gctggagctg
      841 ggcggcaatg cggctatggt ggtccacagc gactgggcgg acctggaggg cgcggcgaag
      901 aaggccgtgc ttgcgggaac aagctacgca gggcagagct gcatctcagt gcagcgcgtg
      961 tatgtggagc gtccggtctt tgatgaattt gttcagcacg cgatgaggca cacaggactg
     1021 ctggtgctgg gcgatccggc gaatgaagaa accgatgttg gcccggtgat acgcgagtcc
     1081 gatgcagagc gcattgaaag ctggatagat gaggcacgcg cggcaggcac gacggtgctg
     1141 acaggcggat cgcgcgaagg cagcctggta gagccaacac tgctgacggg cacgcgtcgc
     1201 ggaatgaagg tactcgatga agaagtgttt gctccggtgc tggcgattga gccgtatgac
     1261 gatttcggtg ctgttctgga tgaggtgaac cgctcgccct acgggttgca ggccggactg
     1321 ttcactcgcg atgtaggacg catcttccgg gcttatgaag cgctggacgt aggtggcctg
     1381 attgtgggcg acacgccgac atggcggttg gacccgatgc cttatggggg agtgaaggat
     1441 tcaggtctgg ggcgtgaggg gcttcgctac acgatggagg aaatgaccga gccgaagctg
     1501 ttggtaatgg cgctttgaca gcgttggatc tcgaattcag tgttgcaaca tccaatgtcc
     1561 gtcatttcgg gctgatccca ggcctgaaca tcgtatagtt ttaaaattta aaaacgatgt
     1621 ccaacaaact catgcagatc gagccgcagg ggccggcgac agaacttatc tataacgact
     1681 ggtacccggc gatgcgcagc gatgggctgc acgggaagac gctcaagaca acgctgctga
     1741 tgggcattcc gatggcgctg gggcggcggg cagacgggcg catttttgcc atgcgcgacc
     1801 tttgtccgca ccgcggcatt ccgctgtcgt atggatggtt tgatggccag aagctgacct
     1861 gcaagtatca tggatggtcg tttgagccgt gcagcgggca atgcgtggag attccgtcgc
     1921 tgaccagccg cgacactctg gacccgaagc gcatttacgg gacggcgttt ccgtgcgaag
     1981 agcgtgatgg atatgcgtgg gtctatgtgc cggagccggg ttcagggcgt gtgcgggaga
     2041 gtgatgagtt gccgcctgtt ccggagttgc cgaagttcag cgccaagttc cgcagccagc
     2101 accttacggc ggagctgcct tgcaatgtgg accacggcat cattggacta atggacccgg
     2161 cgcacgggcc atttgtgcac cagtcatggt ggtggcgcaa gcaggcgagc atccacgaaa
     2221 aagaaaagca c
//
LOCUS       DASRBV010000323         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_693734, whole genome shotgun sequence.
ACCESSION   DASRBV010000323 DASRBV010000000
VERSION     DASRBV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693734"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFA02_11935"
     CDS             <1..423
                     /locus_tag="VFA02_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10603.1"
                     /translation="AELKALVPVRAPVVQERIETELRTASGITDGKGKYVAGVVLITA
                     GYSAEGKYSHFLIAQVPLKIGSAQLAPGEYLIGWKHHTEDSLNVSFYEATSGRMLGSV
                     EAVRNPSITRVESFRIWPPGEKSLMQIGRFTFPYQIEN"
     gene            506..1693
                     /locus_tag="VFA02_11940"
     CDS             506..1693
                     /locus_tag="VFA02_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZU10604.1"
                     /translation="MPDARTQKILAALEENWQAEIHGARTYQALAARENDPIRQKTLE
                     HLAQAEEEHAALWAQRIRELGGEDPVYRGSSAGDADSLANRLGGIDMVLRRLEIDESR
                     DIARYGQQLKELGDGPSIAILERVIEDERDHYQQLSSLIRRRYPRTTIQTADAKSQLE
                     ELLAKRGGQGRKAAGWIGDAIYGVNDGLGAIFGIVSGVSGATLGNSHYVLLAGIAGMI
                     ASALSMGSGAYLAAKSEREIYEAEVERERDAIRMNAPEARELLSLYYQVKGIPAEDAD
                     HIVEHLAKDQDQFLRALASERLNATEEALPKPFVSAFSGAVSTAIGAFVPVIPFFFLT
                     GYPAVITSAIVSLLAHFAVGAAKSLITVRSWWSSGMEMTLVGAVEGVVTYVIGIGLGH
                     IGA"
     gene            1774..2697
                     /locus_tag="VFA02_11945"
     CDS             1774..2697
                     /locus_tag="VFA02_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HZU10605.1"
                     /translation="MSTGRNTGLISEYLMYLRVEKGLRPLSCVAYERDLLQFAEYLER
                     ENVLLATARQEHAAGFLENLRRHGVESRSIARKLSCLRGFYKWLLLDKRIRHDPTVNL
                     ESPDAWKILPKSLAAREINSMLEQAAQAADYPQAGAVALRDRAILELLYAGGLRVSEL
                     IGLHVADLSLDAGRALVRGKGDKERIVPLGAPCIRAIQQYLERGRPQLTRNNRANELF
                     LSVRGKPLTREWVWRLVKASDSHASPHKLRHSCATHMVEHGADLRTVQTFLGHADIAT
                     TQVYTHLALGRLKAVHRQHHPRARRAGGPSS"
     gene            2699..3637
                     /locus_tag="VFA02_11950"
     CDS             2699..3637
                     /locus_tag="VFA02_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZU10606.1"
                     /translation="MSQPGPNSHLDALVSEYLSVLANERGTSPHTLRAYERELRSFTA
                     YLIKTFGGDADIRCVEHQHVRSYLGTLYAKGLSKASTARALAAVRSWFRWLAKTGRVE
                     QSPAALVATPKLPKHLPRVPTIEEMNRAVEGVSEEAAAWPNRDRVIFELLYGCGIRNA
                     ELVGLDLTDIHWANEAILVRGKGRKERYVPLGDAAAQALRTYLPEREARLQAAGKQFH
                     ALLLNAKLRGQGRLTTRSVGRIVKTLAIANGLSAEVHPHTLRHAFGTHMLEEGADLRA
                     IQELLGHERLSTTQRYTQLTTGQVAEVYDRTHPRAK"
     gene            complement(3650..3829)
                     /locus_tag="VFA02_11955"
     CDS             complement(3650..3829)
                     /locus_tag="VFA02_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU10607.1"
                     /translation="MKIIELVEAARRAWRTCGKGNHRASLNFGDCFAYALAKISGEPL
                     LAKGDNFKQTDIELC"
     gene            complement(3826..>4715)
                     /locus_tag="VFA02_11960"
     CDS             complement(3826..>4715)
                     /locus_tag="VFA02_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU10608.1"
                     /translation="VEQLKQYQASHLQSDPMLHTTMLKGFMDGSLGSRTAALKAPYSD
                     DPGNSGIPRYDQQKLNQMAIERAQAGFQMGFHAIGDRAVSMALDAFAAAQNAGPLRAN
                     MKTLHVGTRKVIHATLTGQRNRIEHSQVVDPADFARYKQLGVIASMQPNHLLTDMNWA
                     EQRLGPERSEYAYAWKSFLDAGVPLAFGTDYPVEPITPFRGLYAAVTRMNEAGTRSFH
                     PEQKISIWQALYAYTQGPAYAEFMEQKKGRLANGYFADFVVLDRDITAVAPPEILHTR
                     VLRTVMNGKTVYAAGMKSK"
BASE COUNT          980 a         1360 c         1423 g          952 t
ORIGIN      
        1 gcagagctga aggcactggt tcccgtccgc gctccggttg tgcaggagcg cattgaaacc
       61 gagcttcgca ctgcatccgg tatcacagac ggtaaaggca aatacgtcgc cggggtggtg
      121 ctgattacgg ccggttattc agcagaaggg aagtactcgc atttcctcat cgcccaggtg
      181 ccgctgaaga tcggcagcgc ccagcttgct cccggtgaat acctcatcgg ctggaaacac
      241 cataccgagg attctctgaa tgtgagcttt tatgaggcca caagtggcag aatgctgggt
      301 agtgtagagg ccgtaagaaa tccttccatc acgcgcgtag agtcattccg catctggcct
      361 ccgggggaaa agtccctgat gcagatcggc cgcttcacgt ttccatacca gattgaaaac
      421 taatccccgg aacgtggtgg aaaacacggg ccaaagcggt cgcgatttgc ctttttcctg
      481 atctgctcta aattaagaac gaccgatgcc cgacgcaagg acccaaaaaa tactcgccgc
      541 gctggaagag aactggcagg cggaaatcca cggcgcgcgc acctatcagg cgctggcggc
      601 gcgcgagaac gacccgatac ggcagaaaac tctggaacat ctggcacagg cagaagaaga
      661 gcacgctgct ttgtgggcgc agcgcatccg cgaactcggc ggagaagacc cggtgtaccg
      721 cggcagctcc gcgggtgacg ccgattccct ggccaaccgt cttggcggga tagacatggt
      781 cctccgccgc cttgaaattg acgagagccg ggacatcgct cgctatggcc agcagttgaa
      841 agaactgggt gacgggccga gtatcgccat cctggagcgc gtcattgaag atgagcgcga
      901 ccactaccag caactgagca gtctcattcg caggcggtat ccgcgaacca caattcagac
      961 cgccgatgcc aagtctcagt tggaagaact cctggcaaag cgcggcgggc aggggcggaa
     1021 agcagcagga tggatcggag atgccatcta cggcgtgaat gacggactgg gcgccatatt
     1081 cggcatagtt tccggagttt ccggcgcgac cctcggcaac agccattatg tgctgctcgc
     1141 tggtatcgcc ggaatgatcg ccagtgcgct ttcgatgggc tctggggcgt atctggcggc
     1201 caagagcgag cgcgaaattt atgaggccga ggtcgagcgc gaacgcgatg ccatccgcat
     1261 gaacgcccca gaggcccgcg aactgctgtc gctctactac caggtgaagg gcattcctgc
     1321 tgaagatgca gaccacatcg tcgagcatct tgccaaagac caagaccagt tcctacgcgc
     1381 tctggcctca gaaaggctga acgcaaccga agaggccttg ccgaaacctt ttgtctccgc
     1441 gttttccggc gcagtctcga ccgccatcgg agcatttgtt cctgtcattc cgttcttctt
     1501 tctcactgga tatccggcag tgatcacttc ggccattgtc tcgcttctgg cccactttgc
     1561 tgtgggtgca gccaagtccc tcatcaccgt ccgctcgtgg tggagcagcg gcatggaaat
     1621 gaccctggtt ggcgctgtcg aaggcgttgt cacctatgtc atcggaatcg gactgggcca
     1681 cataggtgca taaaggccgg ctgctgatga attaggttcc cgtgcagacg cgggacattc
     1741 cggggccatc atcgaacgca gataattgag atagtgagca caggacgcaa caccgggctt
     1801 atcagtgaat atctgatgta tctgcgcgtg gagaaagggc tgcgtccatt gtcatgcgtg
     1861 gcctatgagc gcgatctgct gcaattcgcc gaatatcttg aacgcgaaaa tgtgctgctg
     1921 gcgacagcgc ggcaggagca cgccgctggc tttctcgaaa atctccgccg tcatggtgtc
     1981 gagtcgcggt ctattgcacg gaagctctca tgcctgcgcg gcttttacaa gtggctgctc
     2041 ctggacaagc gtatccggca cgatccgacc gtgaatctgg agtcccccga cgcatggaaa
     2101 atcctaccga aatcgctggc tgccagggag atcaattcca tgctggagca ggctgcgcag
     2161 gcggcggact atccgcaagc cggtgcagtc gcgctccgcg accgcgccat cctggaattg
     2221 ctctatgccg gcggcctgcg tgtttctgaa ctcattggtc tgcatgtggc tgacctgtcg
     2281 ctcgatgcgg ggcgtgcttt ggtgcgcggc aaaggcgaca aagaaaggat cgttcctctg
     2341 ggcgcgccat gtatccgggc cattcagcaa tatctggaac gagggcgccc tcagttaacc
     2401 cgcaacaacc gcgccaacga gctttttctt tccgtgcgcg gcaaaccgtt aacgcgcgaa
     2461 tgggtctggc ggctcgtgaa agcaagtgac agccacgcca gtccgcacaa actgcgtcac
     2521 agttgtgcca cgcatatggt ggagcatggc gctgatctgc gcaccgtgca gacgtttctc
     2581 gggcacgccg acattgcgac cacacaggta tatacgcatc tcgcgctggg aaggctgaag
     2641 gccgtgcacc ggcagcacca cccgcgcgcg cgcagagcag gaggcccttc atcctgaaat
     2701 gtctcaaccc ggccccaact cgcatctgga tgcgctcgtc agcgaatatc tctcggtcct
     2761 tgccaatgag cgcggcacct ctccgcatac gctgcgggcc tatgagcgcg agctgcgcag
     2821 ctttactgca tatctgataa agactttcgg tggcgatgct gacatccggt gtgtggagca
     2881 ccagcatgtg cgctcgtatc tggggacatt gtatgcgaag gggttgtcga aggcttctac
     2941 agcacgggcg ctggctgcgg tccgttcctg gttcaggtgg ctggcgaaga cggggcgtgt
     3001 ggagcagagt ccggccgcgc tggtcgctac gccgaagctg ccaaagcacc tgccgcgcgt
     3061 gcccaccatt gaggagatga accgcgctgt agagggcgtg tcagaggagg cagcggcctg
     3121 gcccaaccgg gaccgcgtca tcttcgagct gctgtacggt tgcggcattc gcaatgcaga
     3181 gctggtagga ctcgacctta ccgatattca ttgggcgaat gaagccattc tcgtgcgcgg
     3241 caaaggccgc aaagaacggt atgttcctct tggagacgca gctgcgcagg cattacggac
     3301 atatcttccg gaacgcgaag cgcgattgca ggcagcaggg aagcaattcc atgcgctgct
     3361 gctgaatgca aagctgcgcg gccaaggccg cctgaccacc cgcagcgtgg gccgcattgt
     3421 aaaaacactc gcgattgcca atggattgtc agccgaggtg catccgcata cgctgcgcca
     3481 cgccttcggc acacacatgc tggaggaagg ggccgacctg cgcgccattc aggaactgct
     3541 ggggcatgaa cggctgtcta cgacgcaaag atacacgcag cttacgaccg ggcaggtggc
     3601 cgaggtatat gaccgcacgc atccacgggc gaagtaagag gcctttactt cagcacaatt
     3661 caatatcggt ctgtttaaaa ttgtcgcctt tggcgagcag cggttctcct gaaattttgg
     3721 caagggcgta agcgaagcaa tcaccgaaat tcagcgatgc acgatgattt cctttgccgc
     3781 atgtgcgcca tgccctgcgg gcggcttcaa ccagttcaat aattttcact tagacttcat
     3841 cccagccgca tacaccgtct tcccattcat caccgttcgc agcactctcg tgtggaggat
     3901 ttcgggtggg gcaacggccg ttatgtcgcg gtcaaggacg acaaaatcgg caaaatagcc
     3961 gttagccaac ctgccttttt tctgctccat gaattcggcg tacgcggggc cttgcgtgta
     4021 ggcgtaaagc gcctgccaga tgctgatctt ctgttccgga tggaagctcc tggttccggc
     4081 ttcattcatg cgggtgacgg ccgcgtagag tccgcgaaaa ggtgtgatgg gttcgaccgg
     4141 ataatccgtg ccaaaggcca gcggtacgcc cgcatccaga aacgacttcc aggcataggc
     4201 gtattccgag cgctccgggc cgagacgctg ttcggcccag ttcatgtcgg tgagcaggtg
     4261 attgggctgc atcgaagcaa tcacgccaag ctgtttgtag cgtgcgaaat ccgccgggtc
     4321 taccacctgc gagtgctcaa tgcggttgcg ctggccggta agcgtggcgt ggatgacctt
     4381 acgcgtgccg acgtggagcg tcttcatgtt tgcgcgcagc gggccagcat tttgcgcggc
     4441 ggcaaaggcg tcgagggcca tactgacggc ccggtctcca atcgcatgaa agcccatctg
     4501 gaagcctgcc tgcgcgcgct cgatggccat ctggttgagc ttctgctggt catagcgcgg
     4561 aatcccagaa ttaccgggat catcgctata cggcgctttc agcgcggccg tgcgtgaacc
     4621 aagcgagccg tccataaagc ccttgagcat cgtcgtgtgc agcatcgggt cgctttgcag
     4681 atgcgacgcc tggtactgct tcagttgttc taccg
//
LOCUS       DASRBV010000324         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_693808, whole genome shotgun sequence.
ACCESSION   DASRBV010000324 DASRBV010000000
VERSION     DASRBV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693808"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFA02_11965"
     CDS             complement(<1..209)
                     /locus_tag="VFA02_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10609.1"
                     /translation="MTKEPRPRPPLYWQSQLLLLIVVPVSCMWIFFQTVDAWERRSPM
                     LLQTVGICIAFGLIVWLTRAATTPAV"
     gene            320..1837
                     /locus_tag="VFA02_11970"
     CDS             320..1837
                     /locus_tag="VFA02_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10610.1"
                     /translation="MRSLTGRERPWFAAGALLALFAAAGCGNQYRPVVTPVTPSGPAS
                     QPQAFLVAFSQPNLVQYGQTSGLTPPNSLCPATAAAYNSAGIVTLIDFSGDSIMAQAN
                     VGAGPLTFALDPSGSTAYSVNVTEGTDSNGNPTGKCTSTLSTVPLSTNLQSNKVQNTT
                     LPAANDVATIPTNILTTSSGQYVVEPFTSDLNGAPAIGVLTGSPPSLKLEIPVAPGLI
                     NAIGTSSGQRIYAISQGNSNGGNVAWGQCDTPSSVTTNGEADGIDLATNTVTSQLQVG
                     TCPVYGLMTADGRRAFILNRGSGTITVIDSIKNALDTNSPSQYMKNATIPVGAGPVYA
                     DIYSPSALLVTANYDDDSVSIIDVSINGAVQGYTDTANFGHVLATVKLPAGSHPAAVT
                     VLQDGSRAYTANEGNGTVSVINLSSFTVEKNIQVNGNPRSIVSTFNYPTGKVYTIAQN
                     SPYVTVIRTDTDIVSSSILLQGNGVDLHTTAQYAGTSGTGANSITQSRSVGSGVP"
     gene            1931..3139
                     /locus_tag="VFA02_11975"
     CDS             1931..3139
                     /locus_tag="VFA02_11975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU10611.1"
                     /translation="MSKSENAGRKIKLAYFVSHPIQYQAPMLRRLQQEENLDVTAFFF
                     SDRSVGGYWDPGFKRTVEWDVPLLGGYRHEFLPQLLKEGMDVGGFFRPFNGGTIRALR
                     KHKFDLVWMHGYATLNSLVVMAAAKFFGAKVLLNADSSSLVNRNSRLKEMAKRAYMRV
                     VRSLVDCTFVAGQANHAYWRRYFGDKMIYQPFPYSVDNAYFRERIARVNVPEVKKRLG
                     LEDGVPVILYASKLQKRKRCVDLVLAYQEMLRRTGNEHAPYLLIAGDGEERQELEQRV
                     ADAGLSRVRFLGFQNQSQLPDLFAACDVFVLPSENEPWGLIVNEVMNAGRPVVVTDQV
                     GCHPDLVRPDENGYVYRAGDIAALSEILKKLTSDEDLRRRMGEESLKIIDQHSYEQGV
                     AGLQAFIRGL"
     gene            3191..3448
                     /locus_tag="VFA02_11980"
     CDS             3191..3448
                     /locus_tag="VFA02_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10612.1"
                     /translation="MTIPALAWSVRASSPEYDYARLFQPDKDAARGMLRPEEENPQYQ
                     TRPDTRPFTERHPALLWIALVAAVAILGSIALQTAKAGRVK"
BASE COUNT          821 a          884 c         1019 g          765 t
ORIGIN      
        1 accgccgggg tggtcgcggc gcgggtaagc cagacaatca ggccaaaggc gatgcagatt
       61 cccacagttt gcagaagcat tggactacga cgttcccacg cgtcaaccgt ctgaaagaat
      121 atccacatgc aggagacagg cactacaatc aggaggagca actgcgattg ccagtagagt
      181 ggcggacggg gacgtggttc ctttgtcatc ggtctcctga taaatgaatg cgcctgagtg
      241 atttacaatc taatttctaa caggacttct ttgtcttttt cttttaaaca ggatttgttg
      301 ctgagtgagg tatcaaagag tgcgaagtct tacagggcgt gagcgcccat ggtttgccgc
      361 cggggcgctg ttggccctgt tcgccgctgc tggatgcgga aaccagtatc gtccggtcgt
      421 gacgccggtc accccatccg gccctgcgtc acagccccag gcatttctgg tggcgttttc
      481 gcagccgaac ctggtgcagt atggccagac ttccggctta actcccccca actcactgtg
      541 cccggccacc gctgcggcct ataacagcgc cggaattgtc acgctgattg acttctcagg
      601 cgacagcatt atggcccagg ccaatgtcgg cgcaggtccc ctgacctttg cgctggatcc
      661 ctctggctcg actgcttaca gcgtcaatgt gactgaggga acggatagca atggtaatcc
      721 tacggggaag tgcacaagca cgctcagcac cgtaccgctg agcacaaatc tccaatccaa
      781 taaagtgcaa aataccacat tgccggcagc caatgatgtt gcgacaatcc cgactaacat
      841 ccttaccaca agctctggtc aatacgtggt cgagccgttt acgtctgacc tgaatggcgc
      901 gcctgcgatt ggggtgctta cgggaagtcc accatcgtta aagctggaaa ttccagtagc
      961 gccgggattg attaatgcaa tcggcacgag cagcgggcag cgcatttatg ccatcagcca
     1021 gggaaattcg aatggcggaa acgtagcgtg ggggcagtgt gatacgccct cttcagtaac
     1081 gacaaacggc gaagctgacg gaattgatct tgccaccaac acggtaacaa gccagcttca
     1141 ggtagggacc tgcccggtgt acggattgat gacagccgat gggcgcaggg ccttcattct
     1201 gaaccgtggc agcgggacga ttacggtcat agacagcatc aagaatgcgc tggatacgaa
     1261 ttcccccagc cagtacatga agaatgcaac gatacctgtc ggtgccggcc ccgtgtatgc
     1321 ggacatctat tccccctcgg cgttgctggt gactgcaaac tacgacgatg attctgtcag
     1381 cattattgat gtgagcatca atggagcggt gcagggctat accgatacgg cgaacttcgg
     1441 tcatgtgctg gccacggtga agctgcctgc ggggagtcat cctgcagcag tgactgtatt
     1501 gcaggacgga agccgcgctt atacagccaa tgaagggaat ggcacggtca gcgtcatcaa
     1561 tctgtcgagc tttacagtag agaagaacat ccaggtgaat ggtaatccac gctcgattgt
     1621 ttcaacattc aattatccaa ccggcaaggt ctatacgatt gcacagaaca gtccgtatgt
     1681 aacggtcatc cgcacagata cggacattgt ctcgtcgtct atcctgttgc agggaaatgg
     1741 cgtggacctg catacaaccg cacaatatgc aggaacgagc ggcacgggtg ccaacagcat
     1801 cacgcaaagc aggtccgtgg ggtcgggcgt tccatagaaa ccaccgatct ccgcttttga
     1861 gagggcgtcc catcgtggac gccttttctc ttgtacttta taagtaaaac cgcagacgga
     1921 gcgccaacgc gtgagcaaat ctgaaaatgc aggccgaaag atcaagctcg cttattttgt
     1981 cagccatccg atccagtacc aggcgccgat gctgaggcga ttgcagcagg aggagaacct
     2041 tgatgtgacc gcttttttct tcagtgaccg gtcagtcgga ggctactggg accctgggtt
     2101 caaacggacc gtagagtggg acgtaccgct gctgggtgga taccggcatg aatttcttcc
     2161 gcaattgctg aaagaaggca tggatgtggg cgggtttttt cgtccattca atggcggaac
     2221 catccgtgcg ctgcgaaagc acaagtttga tctggtatgg atgcatgggt atgcgacttt
     2281 gaactcgctt gtagtcatgg ctgctgccaa gttttttgga gcaaaggtgc ttctgaatgc
     2341 agactccagt tcactagtga atcgaaacag ccgcctgaaa gaaatggcaa agcgcgccta
     2401 catgagggtg gtgagaagcc ttgtggactg cacctttgtt gccggacaag ccaaccacgc
     2461 ctactggcgc agatattttg gcgacaagat gatttatcag ccatttccat attccgtgga
     2521 caatgcatac tttcgcgagc gtattgcccg ggtcaacgtt cctgaggtga aaaagcggct
     2581 gggtttggaa gatggagtac ctgtcattct ttatgcttca aagctgcaaa aacgcaagcg
     2641 gtgcgtggac ctggtcctgg cctatcagga aatgctgaga aggacgggaa atgagcacgc
     2701 gccctatctg ctgattgccg gagatggaga ggagcggcag gagttggaac agagagtagc
     2761 agatgcgggg ctgtcgcggg tccgattcct ggggtttcag aaccagagcc aattgcccga
     2821 cttgtttgct gcctgtgatg tatttgtgct gcccagcgaa aacgagcctt ggggactgat
     2881 cgtgaatgag gtcatgaatg caggccgtcc cgtcgttgta accgaccaag tggggtgcca
     2941 tcctgattta gtcaggcctg atgagaatgg ctatgtgtat cgcgcaggag atattgccgc
     3001 gttgagcgaa atactgaaaa agctgacgag cgatgaggac ctgcgccggc gcatgggaga
     3061 ggagagcctg aaaattattg accagcacag ttacgagcag ggtgttgccg gattacaggc
     3121 atttattcgt ggcttataga ggaccattcg ccgagcgggg acatctcgaa accttcgcgg
     3181 cgggcggaag atgacaattc ctgctctggc atggagcgtg cgtgcgagtt ctcctgagta
     3241 tgactatgcg cggctttttc agcctgacaa agatgcagca cggggaatgc tcaggccgga
     3301 agaggaaaat ccgcaatatc agacgcgccc ggacacgcgg ccttttacag aacggcatcc
     3361 tgcgctcctg tggattgcgc ttgtagcggc cgtagcaatc ctgggaagta ttgcgctgca
     3421 gacagcgaag gcgggccggg tgaaataagt cgaatggccg tcccactctt ttggcggccc
     3481 cgactggct
//
LOCUS       DASRBV010000325         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_631948, whole genome shotgun sequence.
ACCESSION   DASRBV010000325 DASRBV010000000
VERSION     DASRBV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631948"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFA02_11985"
     CDS             complement(<1..294)
                     /locus_tag="VFA02_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HZU10613.1"
                     /translation="MRGGAQSHLMLGSDGQPYVVKFQNNPQHVRVLANELLATKLAEM
                     AGLSVPPCDVVEVTNWLIENSKDMIMDFGRHQEQCRAGLQFGSRLVGGLMPGQV"
     gene            complement(441..1769)
                     /locus_tag="VFA02_11990"
     CDS             complement(441..1769)
                     /locus_tag="VFA02_11990"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HZU10614.1"
                     /translation="MNRAQEEFERRRGRENIVLKSRQMGISTWIAGRFFLKTITHPGT
                     LTLQIAHTQEAAEGIFRIVHRFLENLPERMRVGPLRTSHASARQIVFPEIDSEYRVES
                     AGDLNAGRGLTIQNLHCSEVARWPGDASEILQGLRAALPSTGELVLESTPNGAEGCFW
                     DEWNKAEETGMVRHFFPWWWEDAYRAEAVPEESLTEEELELMERHGLDREQIGYRRKI
                     KAGFRGLAKQEYPEDAMECFLTSGECFFDVGAVDARAKEAPEPIETRMEGKLLIWFPP
                     VRGKQYLVAADTAGGGSEGDYSVAQVLDKTNGLQCAELHARLPPLELAQSLAKLAQEY
                     NGALVVVERNNHGSGVLAYLHSVYKYEHIYQYKGQDGWPTSTTERPNMLNLVATALVE
                     NPEIFSSRRLLQECRSFVRLPNGKAGAKSGSHDDCVMAMAVGLAARQTVR"
BASE COUNT          450 a          747 c          512 g          496 t
ORIGIN      
        1 cacctgtccg ggcatcagcc cgcccaccag gcgcgatcca aactgcaggc ccgcgcggca
       61 ctgctcctga tggcgcccaa agtccatgat catgtctttg gagttttcaa tcagccagtt
      121 ggtaacttca accacatcgc acggaggaac actcaatccg gccatctccg ccagctttgt
      181 cgccagcagc tcgttggcca ggacccgcac atgctgcgga ttgttctgaa acttcaccac
      241 ataaggctgc ccgtctgagc caagcatcag atgactctgt gccccgcccc gcatcttccg
      301 tacagcctgt atcgccagaa cagccaactc gccctcgttt caaatcggat tgtaatttat
      361 gtcttgtgtc ctcagttaaa agcggtgagc gcagctgtcg gcggtgtcca ctaagagatc
      421 cgccaacgtg aagcgctgcc ctatcggacc gtctgccgcg ccgccaaccc cacagccatc
      481 gccatcacac agtcatcgtg cgacccgctt ttcgccccag cctttccatt aggaagccgc
      541 acaaaactcc ggcactcctg cagcagtctc cggctggaaa agatctccgg attttctacc
      601 agcgcggtcg ccaccagatt cagcatgttc gggcgttccg tcgtggaagt cggccatcca
      661 tcctgccctt tgtactgata aatgtgctca tatttataca cgctatgcag atacgccagt
      721 acacccgacc catgattgtt gcgttccacc acaaccagtg cgccgttgta ctcctgcgcc
      781 agcttcgcca gactctgggc aagctccagc ggaggcagcc tggcgtgtag ctcagcgcat
      841 tgcagaccgt tggttttgtc aagcacctgc gcaactgaat agtcaccctc actacctccg
      901 cctgcggtgt cagctgccac cagatactgc tttccgcgta caggaggaaa ccatatgagc
      961 aatttccctt ccatacgcgt ttcaatcggc tccggggcct ctttggcccg cgcatccact
     1021 gcgcccacat caaaaaagca ctctccgctt gtcaggaagc actccatggc atcttccgga
     1081 tattcctgct tcgccagtcc gcgaaacccc gccttgattt tgcgccggta gccgatctgc
     1141 tcgcggtcca gcccgtgccg ctccatcaac tcgagttctt cctctgtcag tgattcttcg
     1201 ggcacggcct cagcgcgata tgcgtcttcc caccaccacg gaaagaagtg ccgcaccatc
     1261 cctgtctctt cagccttgtt ccactcatcc caaaagcacc cctccgcccc attcggcgtg
     1321 gactcaagca ccagttcgcc agttgaaggc agcgcagcgc gcagtccctg cagaatctcc
     1381 gacgcatccc caggccagcg cgccacctct gagcagtgca gattctgaat cgtcagcccg
     1441 cgccctgcat tcaggtcccc tgcgctctcc acgcgatact cgctgtctat ctcaggaaaa
     1501 acaatctgcc gcgcgctggc atgagaggtc ctgagcggcc ccacgcgcat cctctccgga
     1561 aggttctcca gaaaccggtg cacaatgcga aagatgccct ctgccgcctc ctgcgtgtgc
     1621 gcaatctgta gtgtcagggt gcccggatgg gtaatcgtct tcagaaaaaa tcttcccgca
     1681 atccacgtgc tgatccccat ctgccgcgac ttcagtacga tgttctcgcg cccgcgccgt
     1741 ctttcaaact cctcttgcgc gcggttcata atcagcggta ctatctttcc ttcacgatta
     1801 cggatgcgca gccagctttc cgccaggaaa atgaaggttg gctcatgcgc cagccttctg
     1861 ttcctttcct tgaaagcctt tcccatgtcg agtaattcag catgatcctc ccctttccat
     1921 tcgctcctca tataaagaaa caatcagctc acaatgttag aaagctttga ttcaggctgg
     1981 gacttaaaaa gtacagccct catctgacag gccgggtctc ccgaatcttg aaagagatgt
     2041 gggcttacag aacttctgct gatccgctaa tacgcatacc agttgtagtc cggcgcgcag
     2101 atgagtgtcc gtttccccgc ggacgaaatc aacagcgacg tccccgtcgt cccctgataa
     2161 ttaatcgtct gcgagctgac cgtcgtcagc gtcactgcgt tcgag
//
LOCUS       DASRBV010000326        11537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_172514, whole genome shotgun sequence.
ACCESSION   DASRBV010000326 DASRBV010000000
VERSION     DASRBV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172514"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFA02_11995"
     CDS             complement(<1..1145)
                     /locus_tag="VFA02_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10615.1"
                     /translation="MTELRRISYLWKDPEAVQGYSTGVSLHSHTNQSKETLDFLANLG
                     AQYRWIRPILHAGESRSREHNVSVNYAAAYWTPPLTPRLAFDLESRQIEKLGLMPLVS
                     LTDHDNINAPMLLRTVASARHIPVSVEWSAPFGGDQSFHLGIHNLPSDSGAMWMKIFE
                     EYTANPAEKKLTGILAALHDLPNVLIVFNHPMWDLYLIGDAKHQQRVQDFMAANHEFM
                     HALELNGLRHWQENRAVKKLAQQWGKLLISGGDRHGTEPNANINLSHAATFTEFVHEV
                     RYEGRSHVLFMPQYAQPWKHRILESTLDAIRDYPEFPQGSRHWDERVFHPDCKGTIRP
                     LRELWPTGQAPFYLSMGIKAVRLMGSRPVAGGLRMAWNESHELKFALG"
     gene            1548..5189
                     /locus_tag="VFA02_12000"
     CDS             1548..5189
                     /locus_tag="VFA02_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10616.1"
                     /translation="MKQKAVVSGLVAWIVFLAGTAFGQLAGKGSIQGTVTDPSGAVVS
                     GATVTATNTATNVKSETKSTGAGDFQFSLDPGVYTVTTTAPGFKSVTQENITVNALEA
                     VTVNTVLQTGSASETVTVTAAPPVIETSNAMVGATMQQEIYSSLPVLQNGTQRRATDF
                     IALMPGVNAQPTNGGLDTNVGVVNGSGSRGAVSGIYINGVPITSVAGEGDPRFIWTSM
                     PLDSINQFQVYTAAYPAMYEGQGVVNYDVKSGSNQIHGSLFEFFRNTALDTWGYLAPA
                     AVNPLTGKATKPAEHQNEYGITLGFPIIKDKLFVFGSYDGYRYIAPPNYQYQTIPTLA
                     MRQGDFTGTGYNIYDPASTTCTTGNVCTRKQFMGVKNGVATPNVIPSNMFSHAAQYMQ
                     QFLPQPTNSAVSNNYLTGPVTGRNNWSTANRIDWHISPSHTISGVISFGRQSTTGAGG
                     GISGALNTNQNQAAPPFISYQQFFVKTKVFIFEDDYVFSPHVVNQFKYAFGRYDSTGL
                     NQDISPTWAASAAGITGLPAGQASQSFPTVSFSGNSQNMNRWAGYSGNRNVANGYVAL
                     DNVQWQLGKHTLTFGGQIAWMQYNFTNNSTGTNPLQLTFNSSETAKFKSGSTLDTATG
                     FAYASYLLGAVNSGTFTQSAAPETGARFRPISPYVQDDWKITPRLTVNLGLRWDYFPS
                     YREAEDRLSWMDPGKTNTLVNYPGALVFAGHGDGKCNCSTNVHDYYKNFGPRIGFAFQ
                     SDPKMVWRGSWGVFYTHGNNVGGSALSRQGAGLQGYSTSPKTTVTLPSPGQTGTQYWQ
                     LDTPFPSYTAPPVFDPTLGTYYTTASTAAAQSVTYADPYYGGRAPQYINWTFGFQRQL
                     TDSMALTMTYVGSEGHFVYVDSNNARGYASNQLDPKYLYLAGQLSNKATAGNLAAAGI
                     SAPFPSFSSSTGTIAQALKPFPQYSGVSDAYGEVGNTSYHALQVMVQQRLSHGLTFMA
                     NYTWSRNMDNSGSFRSGYDIPAAYATDGRFHKARSLDRSLSLSDQPQKFVFTGTYDLP
                     FGKGHLGNDNAIVRALAGGWRLSAIYQAYSGSPIGVVMSSCNTNPSQTTCEPVLNPGF
                     HGPARINGNYGHGANAQTLSSIPYIDSRVFAKTPDYMFSTLARTAPLGLRNSGNYDLD
                     ASLRRSFPLFTERAKLSIQADMFNVTNHTRFGYGASGTTINWGTSSFGTVGVLNNSRD
                     VQLAAHIDF"
     gene            complement(5296..6015)
                     /locus_tag="VFA02_12005"
     CDS             complement(5296..6015)
                     /locus_tag="VFA02_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10617.1"
                     /translation="MLAVLLAAGGCNKKSDQPAQTSPEQNPQSSESVPPPPPDASAQP
                     SSQAAPQRSASASNAGPAPAPAQAAQPLVIPAGTRIRVSLGQTLGSKISQPGESFSAT
                     VADPVVVDGVTVIPAGSPASGTVVAAHALGRFKGAAELAIRLDTVRANGNTYSIATST
                     VERVEKGKGKRTAGFIGGGAGLGALIGGLAGGGKGALIGGLAGAGAGTAGTAFTGNRQ
                     IVIPAETLLTFRLESPVTVRR"
     gene            6157..8424
                     /locus_tag="VFA02_12010"
     CDS             6157..8424
                     /locus_tag="VFA02_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU10618.1"
                     /translation="MKHKRVALAVLGTVVLLLLLLLAALNAFNLNAFFYPHSVGAIFL
                     FTALSVVVFLLFLTLLVLLSRNILKIYADRRSRVLGSRLRSRMLVGALLLSFAPATFM
                     FLFTFGLMNRSIDRWFSQPVSQLRENSTRIALELSHYVTQNARAEAESLASSESVSLN
                     YQAGNTNALLDEIRTHRITLQGGFAVIYRDETPVVEYQLPQTTHPVKVQAWLDTSSPE
                     EAPSQPLPSIIRKAAQRTDEPVIAIGDIEYALGMATTSGGGVIVVGLPLPSGLSGTVA
                     DVRKGAAQYWAMYRERRSIRSTYLLLLLLLTALTFFASSWLAWFFSKQITRPLENLAE
                     AMDQIAEGHYHQRVTISATEELGELLQSFNHMAADLEQSRLIAEQSTKQLFEANATLE
                     ERRRELETILETIPGGVITLDSELRITQANQSFADLLPHGAPHSLKGLQLQKIFPEEI
                     ASELTLLSRRAQRMGVASTEFEMRGAKGKLDLSATMAMLHLAQERRGYVLVLENITEF
                     LQAQRQMAWKEVAQRVAHEIKNPLTPIALSAERIKRHVDRNTPDSPGIIHSCGDVILS
                     SVETLRRLVDQFAALAQFPTAQPKPADLNTIVENALRLFEDRLHGIHLDVRLAPDLPP
                     VMADAEALKRALANLIDNAAEAMQEGRLRILSLTTGLADHREMAEIVVSDTGHGLTDE
                     TRERLFLPYFSTKQRGTGLGLAIAAKIVQDHQGSIRAEQNYPAGARFIIELPLAGSGG
                     SSDSSRATAANKTAS"
     gene            8421..9776
                     /locus_tag="VFA02_12015"
     CDS             8421..9776
                     /locus_tag="VFA02_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU10619.1"
                     /translation="MKHILIVDDEADIRRLLEDILREEGYAVASTGSANEALVLLRDV
                     TYDVLLLDIWLPDRDGLNVLNELRSLDSDNKPEVIIISGHGTIETAVRATKLGAFDFL
                     EKPLSLERTLIVVKNAIEAKRLRSENEEFKRQLSLHAAITGESVAAKALRQQIKLMAP
                     TNGRVLIYGESGSGKELIARAIHAESLRRERVFVELNCAAIPEDYIESELFGYRNSAV
                     PGGPAEKRGTFERADGGTLFLDEVGDMSLKTQAKVLRALDEQRFTPVGAAHAMSVDVR
                     VIAATNKNLEDEIAKGNFREDLFYRLNVIPFYVPPLRDRKEDIPLLTREFLREFGRQY
                     GRPRIDMTEDALDVLKQYHWPGNVRELKNLIERILILNPHTLRIERRHLPMLVHRASG
                     RRNEDFTTLQQAREAYERDYILKKIDECHGNISRAAETLGLERSHLYRKMRALGISVR
                     E"
     gene            complement(9787..9876)
                     /locus_tag="VFA02_12020"
     tRNA            complement(9787..9876)
                     /locus_tag="VFA02_12020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9840..9842),aa:Ser,seq:tga)
     gene            complement(10016..10588)
                     /locus_tag="VFA02_12025"
     CDS             complement(10016..10588)
                     /locus_tag="VFA02_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZU10620.1"
                     /translation="MAHGENKGRIRMLEREHVEGEIAALYDKIYQERGVVPYMFKTVS
                     HVPALALGFAALLKPLMGEGELPAWYKELVATRVAALNTCEYCISSHRYLARLRGATN
                     EQIAAIDSYEAGPFTEKEKAGLRYADKLHTSAHAIDDAAYQAVKQHFSDKEVIELTAV
                     AAAFEFFPRFVSALAIPVTPLPQEAEQAAS"
     gene            complement(10614..10886)
                     /locus_tag="VFA02_12030"
     CDS             complement(10614..10886)
                     /locus_tag="VFA02_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZU10621.1"
                     /translation="MAHTVFCARYKQQMEGLDEPPFDSDFGNKIYHNVSKKAWLEWLE
                     HQKMLLNEYRLQPWTPQAQEFLVEQMDRYFFGEGSELPKEYVPPSH"
     gene            complement(10962..>11537)
                     /locus_tag="VFA02_12035"
     CDS             complement(10962..>11537)
                     /locus_tag="VFA02_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZU10622.1"
                     /translation="IGGIPIELIDTAGLREAADEAESIGIQKSREALADADLVLIVLD
                     ASVPLREDEAELIASLEGRRALVAANKCDLNLEQSELQIPGDGLKLVRTSAITGEGID
                     ELRRVVLELVHGPGAGNENGMLTTVRHHQAVTGALDSLAAARKAVAEKIPHEMLLLDL
                     YAALRHLDSLTGETTPDDILNLIFSTFCIGK"
BASE COUNT         2484 a         3502 c         3123 g         2428 t
ORIGIN      
        1 cccagtgcaa atttcagttc gtgggactcg ttccaggcca tacgcagacc tccggcaacc
       61 gggcggctgc ccatgagccg cacggctttg atgcccatgc tcagatagaa cggcgcctgt
      121 ccagtgggcc acagttcgcg cagcgggcgg atggtgcctt tgcaatcggg atggaagacg
      181 cgctcgtccc aatgccgtga accctgcgga aactcagggt agtcacgaat tgcatcgagt
      241 gtggactcga ggatgcggtg cttccagggc tgcgcgtact gcggcataaa gagcacgtgg
      301 ctgcgtccct cgtagcgaac ctcatggacg aactcagtaa acgtggccgc atgcgaaaga
      361 ttgatgttcg cgttcggctc agtgccgtgg cggtcaccgc cggagatgag cagcttgccc
      421 cattgctgcg ccagcttctt tacggcgcgg ttttcctgcc agtgccgcag gccgttcagc
      481 tccagtgcgt gcatgaactc gtggttggcg gccataaagt cctgtacgcg ctgctggtgc
      541 tttgcatcgc caatcagata gaggtcccac atagggtgat tgaagacgat gagcacattc
      601 ggcagatcgt gcagggcggc cagaatcccg gtcagtttct tctcggcggg atttgcagtg
      661 tattcctcga agatcttcat ccacatcgcg ccggagtcgc tgggcaggtt gtgaatgcca
      721 agatgaaagg actggtcgcc cccaaacgga gcgctccact cgacggacac aggaatatgc
      781 cgggccgatg cgaccgtgcg cagcagcatg ggagcgttga tattgtcatg gtcggtcagc
      841 gaaaccagcg gcatcaaacc gagtttctca atttgcctgc tctcaagatc aaaggccaga
      901 cgtggcgtca gcggtggcgt ccagtaagcg gcagcatagt tgacagatac attgtgctcg
      961 cgtgaacggc tctctcccgc atgcagaatg ggacggatcc agcggtactg ggcgcccagg
     1021 ttggcaagaa aatccagcgt ctctttcgac tggttggtat ggctgtgcag tgaaactccg
     1081 gtggagtacc cctggactgc ttccgggtct ttccacaagt acgagatgcg ccgcagctcc
     1141 gtcatactct cctccgtgcc cgtggtcagt atccagtttg caccatgaaa gcggcattac
     1201 ggccataaaa aaggacggca aatattagaa gcagcgtatc tatctgggca ataatccctt
     1261 cttattgggc ttgaacatag gaaactggtc gtacctctgg ttcgagatcc atgtctgctt
     1321 cgcagaaaaa gcagtctctt ttgggtcatc tatagcctcg agtgttttat caatattcca
     1381 gttcgcgctt gacaatcgta ttttcatgca agcaaactgc atttcatctg gaagtggtct
     1441 gacctcatca acggacccat ttccggaatg caattctttg aagcagttgc cagataagaa
     1501 gggcccgagg ctctcaaaga agacgtaacc atctattgga ggcggatgtg aagcaaaaag
     1561 cagtcgtttc agggctggta gcctggattg tgtttctggc aggcacggcg tttggccagt
     1621 tggccggaaa aggctcgatt cagggcacag tcaccgatcc ctcaggcgca gtcgtaagcg
     1681 gcgccacagt tacggccacc aacacggcca ccaatgtaaa gtctgaaaca aaaagcaccg
     1741 gggctggcga tttccagttt tccctcgatc cgggcgtata cacggtcacc accaccgcgc
     1801 cgggttttaa atctgtcacg caggaaaaca ttaccgtgaa tgcgctggag gcagtcacgg
     1861 tcaacaccgt gcttcagacc gggtctgcct cagagaccgt gaccgttacc gcggcccctc
     1921 cggtcattga gacctcaaac gccatggtcg gcgcaaccat gcagcaggaa atctactcct
     1981 cgctgcccgt cctgcagaac gggacgcagc ggcgcgcaac cgactttatc gcgctgatgc
     2041 ccggtgtgaa cgcgcagcca accaatggcg gcctggacac gaacgtaggc gtagtgaacg
     2101 gctcgggcag tcgcggcgcg gtctcaggaa tttacatcaa cggcgtgccg attacttcgg
     2161 ttgccggtga aggtgacccg cgctttatct ggacctcgat gccgctcgac tccatcaacc
     2221 agttccaggt gtacacggct gcctatcctg ccatgtatga agggcagggc gttgtcaact
     2281 acgacgtaaa aagcggcagt aatcagattc acggcagtct ctttgagttc ttccgcaaca
     2341 ccgccctcga tacctgggga tatctcgcgc ccgcagccgt caatcctctc acaggaaagg
     2401 ccaccaagcc tgccgagcac cagaacgaat acggcatcac gctcggtttt cccattatca
     2461 aagacaagct ctttgtgttt ggaagctatg acgggtaccg ctacattgcg ccaccaaatt
     2521 accagtatca gacgattccg acgctggcca tgcgtcaggg tgatttcact gggaccggat
     2581 acaacatcta tgaccctgcg tcaaccacct gcacaacagg caacgtctgc acacgcaagc
     2641 agtttatggg agtgaaaaac ggcgtggcca cgcccaatgt cattccgtcc aatatgttct
     2701 cgcatgcagc gcagtacatg cagcagtttt tgccgcagcc gaccaacagc gctgtttcca
     2761 acaactatct caccggcccg gtcacaggaa gaaacaactg gtccacggcc aaccgcattg
     2821 actggcacat ctcgccgtcg cacaccatct ccggcgtaat ttcgtttggg cgccagtcca
     2881 ccactggagc aggcggcgga atctcgggcg cgttgaacac caaccagaac caggccgcgc
     2941 cgccgttcat ctcatatcag cagttttttg taaagacaaa ggtgtttatc tttgaggacg
     3001 attatgtctt ctcgcctcat gtggtcaacc aattcaaata cgcttttggc cggtacgatt
     3061 ccaccggact gaaccaggac atcagcccaa catgggccgc ttcagcagca ggcatcaccg
     3121 ggttgccggc tggccaggcc tcccagtctt tccccacggt gagtttcagc ggcaactcgc
     3181 agaacatgaa ccgctgggcc ggatactccg gcaaccgtaa tgtggccaat ggatatgttg
     3241 cgctggacaa cgtgcaatgg cagctcggca aacacacact caccttcggc ggccagattg
     3301 catggatgca gtacaacttc accaataaca gcaccgggac caacccgctg caactgactt
     3361 tcaattcctc ggagacagca aaattcaagt ccggcagcac gctcgacacg gccaccggat
     3421 tcgcatatgc aagttatctg ctcggcgcgg tcaacagcgg aacattcacc cagagcgccg
     3481 cgcctgagac cggagcgcgc tttcgcccca tctcgcctta tgtgcaggac gactggaaga
     3541 ttactccccg actcacggtc aatctcggcc tgcgctggga ctacttcccc agctatcgtg
     3601 aagcggaaga ccgcctctcg tggatggacc ctggcaaaac caacacgctg gtgaattacc
     3661 caggcgcgct ggtctttgcc ggacacggcg acggcaagtg caattgcagc acgaacgtgc
     3721 atgactacta taagaatttc ggtccgcgca tcggctttgc attccagagc gatcccaaaa
     3781 tggtctggcg gggaagctgg ggtgtcttct acacccacgg aaacaatgta ggcggcagcg
     3841 ccctttcacg ccagggggcc gggttgcagg gctacagcac ttcacccaag accactgtca
     3901 cgctgccttc tcccggccag acaggcacgc aatactggca gctcgatacg ccattcccaa
     3961 gctacacggc ccctccagtc ttcgatccca cgctgggcac ctattacacg acggcaagca
     4021 cggcggcagc gcaaagcgtg acctacgccg acccgtacta tggcggacgc gctccgcagt
     4081 acatcaactg gacctttggg ttccagcgcc agcttactga cagcatggcg ctgaccatga
     4141 cctacgtcgg ctcagaaggc cattttgtct acgtggactc aaacaatgcg cgcggatatg
     4201 cctcaaacca gcttgacccg aagtatctgt atctggccgg gcaactgagc aacaaagcca
     4261 cagccggaaa tcttgcagcc gcaggaatca gcgcgccgtt ccccagcttc agctcgagca
     4321 ctggaacgat tgcgcaggcg ctgaagccct ttcctcaata cagtggcgtc tccgatgcgt
     4381 acggtgaagt gggcaacacc tcctaccatg cgcttcaggt gatggtacaa cagcgcctga
     4441 gccacggcct cacctttatg gcgaattaca cctggtcgcg caatatggac aactccggaa
     4501 gctttcgctc cggttatgac atccctgccg cctatgccac cgacggacgg tttcacaaag
     4561 cacgctcgct tgaccgcagc ctttcgctca gtgaccagcc gcagaaattc gtcttcaccg
     4621 ggacctacga tctgcccttc ggcaaaggac accttggcaa tgacaatgcg attgtgcgcg
     4681 cgctggccgg cggatggcgt ctttctgcca tctaccaggc ctactccggc tcgcccatcg
     4741 gcgtcgtgat gagctcctgc aatacgaacc cttcgcagac cacctgtgag ccggtcctca
     4801 acccgggctt ccacggcccc gcgcgcatta acggcaatta cggtcatggc gcgaacgcac
     4861 agacactcag cagcatcccc tacattgatt cgcgggtctt cgccaaaacg ccggactata
     4921 tgttcagcac attggcgcgc accgccccgc tgggtctgcg caactccggc aactatgacc
     4981 tggacgccag ccttcgccgc agtttcccgc tgttcacaga gcgcgcgaag ctcagcattc
     5041 aggcagatat gttcaacgtg accaaccaca cccgtttcgg ctatggcgcc agcggcacca
     5101 ccatcaactg gggcacgtcg agcttcggga ccgtgggcgt gcttaacaat tcacgcgacg
     5161 tgcagttggc cgcgcacatt gatttctgaa agaagtgtgt ggacagatgg tctccatccc
     5221 catcacgcat ggtgttcgcg tgatgggatg gaaccagagg aaggatctca gagtgggtgg
     5281 gtcaccgggt ttctttcatc tgcgtacagt tacaggcgat tccaagcgga aggtcagcag
     5341 tgtctctgca ggaatcacaa tctgccggtt gcctgtgaat gcggtgcctg ccgtgcctgc
     5401 gccagcaccg gcaagtccgc cgatcagcgc tcctttaccg cctcccgcca gcccgccgat
     5461 cagcgctcca agaccagcgc cgccgccgat gaatccggcc gtgcgcttgc ctttcccttt
     5521 ctccacgcgt tcaaccgtgc tggtggcaat cgaataggtg ttgccgttgg cgcgaacagt
     5581 gtccagacga atggcgagtt cggctgcgcc cttgaagcgc cctaacgcat gcgcggccac
     5641 tacggtccca cttgcaggag aacctgcggg aatcaccgtc acgccatcca caacaacggg
     5701 gtctgcgacc gtagcgctga agctctctcc aggctgactg atctttgagc cgagggtttg
     5761 ccccagcgaa acacgaatgc gtgtccctgc gggaatcacc agcggttgtg ctgcctgcgc
     5821 gggagcaggt gctggccccg cattcgaagc agaagcagaa cgctgcggcg ctgcctgaga
     5881 agacggttgt gccgatgcat ccggcggtgg aggaggaaca gattccgacg attgcggatt
     5941 ttgctccggt gatgtctgtg ctggctggtc tgatttctta ttgcaaccgc ccgctgccag
     6001 gagcacagca agcataaacg gcaggatagg aagggtcttt ctgctgactt gcatagcgcc
     6061 taataagatg cggcccaggc cttgctggtt gtgccgctgt cttctttacg atagaagccg
     6121 cttccggaag cctataatct gggaatcaga atgcccatga agcacaagcg cgttgcctta
     6181 gctgttctgg gaacagtcgt cctgctgttg ctgttgttgc tggccgcgct gaatgctttt
     6241 aatctgaatg ccttttttta cccgcacagc gtgggtgcca tctttctttt tactgcgctt
     6301 tctgttgttg tctttctgct ctttctcacg ctgttggtcc tgctctcgcg caatatcctg
     6361 aaaatctacg ctgaccggcg cagccgtgtg ctcggctccc ggttgcgctc gcgcatgctg
     6421 gttggcgcgc tgctgctttc ctttgctcca gccacgttca tgttcctgtt caccttcggg
     6481 ctgatgaacc gctccataga ccgatggttc tcgcagcccg tttcgcaact gcgggagaat
     6541 tcaacacgca tcgcactgga gctgtcgcac tatgttacgc agaatgcgcg cgccgaggcc
     6601 gagtccctgg ccagctctga atctgtttca ctgaattacc aggccggaaa cacgaacgcc
     6661 ctgctcgatg aaattcgcac ccaccgcatc accctgcagg gtggatttgc cgtcatctac
     6721 cgcgacgaaa ctccggtcgt ggaataccag ttaccgcaga ccacccatcc ggtgaaagtg
     6781 caggcgtggc ttgatacatc ttctcctgaa gaggcgcctt cacagccatt gccttccatc
     6841 attcgcaaag ccgcgcagcg caccgatgaa cctgtcatcg ccatcggcga cattgaatac
     6901 gccctgggta tggcgaccac atccggtggc ggagtgatcg tagttggcct tcccttgccc
     6961 tcaggattaa gcggcacggt cgccgatgtt cgcaagggag cagcgcagta ctgggccatg
     7021 tatcgcgagc ggcgcagcat tcgcagtact tatcttttac tacttttgct cctgactgcg
     7081 ctcacgttct ttgccagcag ttggctggca tggtttttct caaagcagat cacccgtccg
     7141 ctcgaaaacc ttgccgaagc catggaccag attgccgaag gccattacca tcagcgcgtc
     7201 acaatctcgg ccacggaaga attgggtgag ctgctgcaat cattcaatca catggcggcc
     7261 gacctggagc agagccgcct gattgccgag cagtccacaa aacaattgtt tgaagccaat
     7321 gcaaccctcg aagagcgccg ccgcgagctg gagacgattc ttgaaaccat tcccggcggc
     7381 gtcatcacgc tcgattccga actgcgcatc acgcaggcaa accagtcgtt tgctgatctt
     7441 ctgccgcacg gtgcgccgca ttcgttgaag ggcctccagc tccagaagat tttcccggaa
     7501 gagattgcca gcgaactgac cctgctttcg cgccgtgccc agcgcatggg cgttgcgtct
     7561 actgaatttg aaatgcgcgg cgccaaaggc aaacttgatt tgtctgccac catggccatg
     7621 ctccatctcg cgcaggagcg ccgcggctat gtcctcgtgc ttgaaaacat cacagagttt
     7681 ctgcaggcgc agcgccagat ggcatggaaa gaggtcgcgc agcgcgtggc gcacgaaatc
     7741 aagaacccgc tcacgcccat tgcgctttcc gccgagcgca tcaagcgcca tgtagaccgc
     7801 aacacgcccg attcgcctgg catcatccat tcatgcggcg atgtgattct gagctcggtt
     7861 gaaacgctgc gcaggcttgt ggaccagttt gccgctctgg cgcagtttcc aaccgcacag
     7921 ccaaaacccg cggacctgaa caccattgtc gaaaacgctt tgcgcctgtt tgaagaccgc
     7981 cttcacggca ttcatctcga tgtgcgtctt gcccctgatt tgcccccggt gatggctgat
     8041 gcagaagcat tgaagcgtgc tctcgccaat ctgatcgaca acgccgcaga ggccatgcag
     8101 gaaggccggc tccgcattct cagcctgacc accggtcttg cggaccatcg cgaaatggcg
     8161 gagattgttg tctctgacac gggccacggc ctcacagatg aaacacgcga acggctgttc
     8221 ctcccttatt tctcgaccaa gcaacgcggc accggactcg gcctcgccat tgcggcaaaa
     8281 atcgtccagg accatcaggg ctcgattcgc gccgaacaga actatccggc gggcgcgcgg
     8341 tttatcatcg aattgccgct ggccggcagt ggcggcagca gcgattccag cagggcaaca
     8401 gcggcaaata agacagcttc atgaaacata ttctgattgt tgatgatgaa gcggacatcc
     8461 gcaggctcct tgaagacatt ctgcgcgagg aaggctacgc cgtcgccagc acaggctctg
     8521 cgaatgaagc gctcgtgctg ctgcgcgatg tgacctatga cgtgctgctg ctcgacatct
     8581 ggctgccgga ccgcgatggc ctcaatgttc tgaacgaact tcgctcgctc gattccgaca
     8641 ataagccgga agtcatcatc atctccggac acgggacgat tgaaactgcg gtccgcgcca
     8701 cgaaacttgg cgcattcgat tttcttgaga aaccgctttc actcgaacgc accctgattg
     8761 tcgtcaagaa tgccatcgaa gcaaaacggc ttcgcagcga aaatgaagaa ttcaagcgcc
     8821 agctctcatt gcatgccgcc atcacaggcg aaagcgtcgc cgccaaggcc ctgcggcagc
     8881 agatcaagct gatggccccc accaatggac gcgtcctcat ctatggcgag tccggaagcg
     8941 gcaaagaact gattgcccgc gccattcacg cggaaagcct gcgccgtgaa cgcgtctttg
     9001 tagagttgaa ctgcgccgcc atccccgagg actatatcga aagcgagctt tttggttaca
     9061 gaaacagcgc cgtccctggc ggcccggcgg aaaagcgcgg cacatttgag cgcgcggatg
     9121 gaggcacgct cttcctggac gaagtcggtg acatgagcct gaaaacgcag gccaaagtgc
     9181 tgcgtgcgct cgacgaacag cgcttcactc ccgtgggcgc ggcacatgcc atgagcgtgg
     9241 atgttcgcgt cattgccgcg acaaacaaaa atcttgaaga cgagatcgca aaaggaaact
     9301 tccgcgaaga ccttttctat cgcctgaatg tcattccgtt ttatgttccg cccctgcgcg
     9361 accgcaaaga ggacattcct ctgcttaccc gcgagttcct gcgcgaattc ggccgccaat
     9421 atggacgccc gcgcattgac atgactgaag atgctttgga cgtgctcaag cagtaccact
     9481 ggccaggcaa tgtacgcgaa ttgaagaacc tgattgagcg catcctgatc ctcaacccgc
     9541 acacattgcg catcgaacgc aggcatcttc ctatgctggt gcatcgcgca tcaggcagac
     9601 gcaatgaaga cttcaccacg ctgcaacagg cgcgcgaagc ttatgaacgg gactacatcc
     9661 tgaagaaaat tgatgaatgt cacggaaata taagccgcgc tgcggaaacc ctcggcctcg
     9721 aacgcagcca cctctataga aaaatgcgcg cgctcggaat cagcgtgagg gagtagcagg
     9781 acaggatggc ggagggagag ggattcgaac ccccgttacc ctttcgggta aagcggtttt
     9841 caagaccgcc tgtttcaacc actcacacat ccctccgcaa gggttggccg gcaacgttgt
     9901 tgccggggta tcgagcggca gggcctttgc cctacttcat tgtacgacgg ccccatcttt
     9961 atagcatttt cgtgtatgga gagtccgttg ttctgacgac caaaagggag aaatctcagc
    10021 tcgcagcctg ctccgcctcc tgcggaagtg gcgtgaccgg aattgccagc gcggaaacaa
    10081 atcgcggaaa aaactcaaaa gcggcagcca cggccgtcag ctcaatcact tctttatcgc
    10141 tgaagtgttg tttcacggct tggtaggctg catcgtctat ggcatgggct gatgtgtgca
    10201 gcttgtcggc gtagcgcagg ccggcctttt ctttctccgt gaagggcccg gcctcatagc
    10261 tgtcaatggc tgcaatctgc tcattggtgg ccccgcgcag cctggccagg tagcggtgcg
    10321 atgaaatgca atactcgcag gtattgagtg cagcgaccct cgttgctacc agctccttgt
    10381 accaggcggg cagttcgcct tctcccatca gcggcttcag aagtgccgca aatccgagcg
    10441 ccagcgctgg cacatgcgag acggtcttaa acatatacgg cacaaccccg cgctcctgat
    10501 aaatcttgtc atagagcgcg gcaatttcac cttccacgtg ctcgcgttcc agcatccgga
    10561 tgcggccctt gttctcaccg tgcgccatag ccagaggtcc ctttccgcaa aactcaatgc
    10621 gatggaggca catactcctt tggcagctct gagccttcgc cgaagaagta gcggtccatc
    10681 tgttctacca gaaactcctg ggcctgcggc gtccacggct gcaggcggta ttcattcagc
    10741 agcatcttct ggtgctccag ccactccagc caggccttct ttgagacatt gtgataaatc
    10801 ttgttgccaa aatcgctgtc aaacggcggc tcgtcgagcc cttccatctg ctgcttatag
    10861 cgcgcgcaaa atactgtgtg tgccatgctt tgtccttctg aaactattgt aagcaatcgg
    10921 atttccaggg ccaaaaaggc cgtggccccg tccttgcctg cttattttcc gatgcagaat
    10981 gtcgaaaaaa tcaggttgag aatgtcatcc ggcgtggtct cgccagttaa cgaatccaga
    11041 tgccgcaacg ccgcatacag atccagcagc agcatctcat gcgggatctt ctctgccact
    11101 gcttttcgcg ccgcggcaag cgaatccagt gcgcctgtaa ccgcctgatg gtgccggacg
    11161 gtggtcagca tcccgttttc atttcctgcg cccggcccat ggaccagttc gaggaccact
    11221 ctacgcaatt cgtctatgcc ttccccggtt atggccgatg tgcgcaccag cttcaaacca
    11281 tcaccgggaa tttgtaactc actctgttcc agattgaggt cgcatttgtt cgccgcgacc
    11341 agcgcgcgcc tgccttcaag agatgcaatc agctctgcct catcttcacg caggggaaca
    11401 gaagcgtcga gcacgatcag gaccaggtcc gcatcggcca gtgcttcgcg cgatttctga
    11461 atgcctatgg attcagcttc gtcagcagcc tcacgcagac ccgccgtatc tatcagctca
    11521 atcgggatgc cgccaat
//
LOCUS       DASRBV010000327        16381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_536776, whole genome shotgun sequence.
ACCESSION   DASRBV010000327 DASRBV010000000
VERSION     DASRBV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536776"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFA02_12040"
     CDS             complement(<1..647)
                     /locus_tag="VFA02_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10623.1"
                     /translation="MKLLSKAVLAAGLGILAGLPALAAQLSEDARAAIPKDVQQLIVV
                     DYRAMQNSDAAMNLKARILPPELKELEQALKTSGLNDNHDIDELAFAAFRSSPSVDYT
                     RIIGVAQGQFSLQDILANFRKKKVKPTKIRDNALYPMGGSGMLVCFLNPTTMVFGSSD
                     SIKYALDARDGLAPNLLSNDSMMQQMSAVDNEPIWSILDQKGTQMMMRGVLGDAS"
     gene            complement(688..1611)
                     /gene="ftcD"
                     /locus_tag="VFA02_12045"
     CDS             complement(688..1611)
                     /gene="ftcD"
                     /locus_tag="VFA02_12045"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZU10624.1"
                     /translation="MTNDALIECVPNFSEGRDARKVLSILSAMRVEGVVLLDWSMDSD
                     HNRSVVTVAGPPAAIVEAAVRAAGRAAELIDLTAQEGVHPRIGAADVVPFVPIRNISL
                     EQCALLARQAGMEIWKRHHVPVYFYEAAAARPDRALLEDVRRGQFEGLREAVRKDASR
                     RPDVGGPDLHPTAGASAVGARRYLIAYNIYLATDNVATARAIAKEIRASSGGLSGVKA
                     MGVLAHGMAQISMNITDFRQTSVASAYAAVKRQAARYRTQPARGELIGLLPEAAFEPE
                     SEWVRQLDGFHPEEKILERRLQHPLEWPAPV"
     gene            complement(1668..2723)
                     /locus_tag="VFA02_12050"
     CDS             complement(1668..2723)
                     /locus_tag="VFA02_12050"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZU10625.1"
                     /translation="MRTIHLKVSSGEIASEAARSTLEQAAAILREGGTVAFPTETVYG
                     LGANALDATAVAKIFAAKQRPSWDPLIVHVADMEMLWRVAKNVPEAAHRLAERFWPGP
                     LTLLLEKKDEVPDEVTAGRRKIGVRMPQHPVAQALIRRAGVPVAAPSANRFGRTSPTS
                     AAHVVEDLDGRIDAVLDAGETWHGLESTVIDAVETPCVVYRPGVVSLEQIRAAGVEAV
                     GFREPEELHETPPEALPSPGVGLRHYAPKAKLVLVDGEGEAQIAAFVDAVERARHQGM
                     KTGLMLPDVFAARLRTEDMAVFDWGRWERAEELAQRLFAGLRFLDQAGAEVILCPVPK
                     AEGIGIAIRDRLRKAAR"
     gene            2835..3419
                     /gene="cyaB"
                     /locus_tag="VFA02_12055"
     CDS             2835..3419
                     /gene="cyaB"
                     /locus_tag="VFA02_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HZU10626.1"
                     /translation="MSAVEIEVKFRVADGEALERKLLSIGFHKVTPRTFEKNILFDTP
                     ERSLRAQQSILRIRQYGERWIVTHKCLPQDHDAAARHKHRVETETEVRDGEAMAIIFS
                     ELGYKPAFAYEKWRTEYADHTGHCVIDETPIGLFAELEGPGEWIDSIGKKLGLDPSEM
                     ITLSYGRLFDEWRKETGSTATDLTFEAIANQKQD"
     gene            3523..3864
                     /locus_tag="VFA02_12060"
     CDS             3523..3864
                     /locus_tag="VFA02_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZU10627.1"
                     /translation="MNSRNFRKSAFGVLWRLALAFACLLIALPGHGEERRVEKRVSPV
                     YPEIAKRMRIGGVVHVEATVAADGSVTAVKAVSGNKMLTPAAEEAVKRWKFAPGDGQS
                     TVGVDINFEVN"
     gene            3902..5629
                     /locus_tag="VFA02_12065"
     CDS             3902..5629
                     /locus_tag="VFA02_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU10628.1"
                     /translation="MTFTQSRLTNKLRLSTGVLIVSILLGSSAAYLKMRQASQLAENV
                     ASEQIPALTAVRDLRVALLDSSNALKAYMLFGIDPSMASRYKAVLEKSKADANDAISR
                     ILAKRQVIDAMAGSGQVDSLLNEYRAFEQGQDHVEAMAIGQGSDATSKAYDLLQGEVA
                     NHEAACRKAGSAVVSAMMQATDASLHETVHSMRLQAIYLWLAILFGGIIGSSLAELTI
                     RRVVRSVVLVADRAQQIAEGDLTGEALHTDSQDEVGSLATSINRMQDNLREMIRTMME
                     ISNTVNSDAEKLAHSASESFRRTKEQSLQTQQAATAMQEMSISIAEVSRHSQNAAENA
                     KEASKIAKEGGSTVEQMLISMQSIADSVRNTASTVQRLGKESEQIIRIVNVIEEIAQK
                     TNLLALNAAIEAARAGEQGRGFAVVAGEVRRLAESTRNATSEIGQMIEGIQVHTRSAV
                     EAMDAGTATVNEGVETTTRAGEALQRIIHMADQVDSMIAQIAAAAMQQSEAARQSSAN
                     VDIINRLGEENAAAIPESNAIVTSVQAGARRLQEHISRFKLEEGGRTVRAAFASQGFA
                     PQTAAAYGD"
     gene            complement(5745..6893)
                     /locus_tag="VFA02_12070"
     CDS             complement(5745..6893)
                     /locus_tag="VFA02_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZU10629.1"
                     /translation="MEQSPPMKSAVKRRNFLKSMGVAGAAISTGALLGNLPLEAEERG
                     GRLDPGDAAILRFLAAAETLEADLWQQYAELGGVQKNEAPYLKGLTTGNTAYTKALTN
                     LDSDMPQYITDNTDDEFSHAAFINEYLKSKGADTVDLTPFRTLPSSKATGAQQINRLT
                     NLMELTVDTSWWTRYRSGQKNPDFGDSFPNAIAVIGTDRHTAIPRNDDEAQLDPSSPN
                     GVTDVTQFIANTAGFHFAFIEQGGTSLYAQLAQRVKSVEVLRILLSIGGTEIAHFQTW
                     QDKAGNCPALTGVHDPVSGKTVDFPDISTFEGNENLQANLIMPEPTVFLSKKFPVVSI
                     IRPTETKRAATAALAAFTADGLFRGQSPGFFAYLRDLAEDADEARRGY"
     gene            7019..7624
                     /locus_tag="VFA02_12075"
     CDS             7019..7624
                     /locus_tag="VFA02_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZU10630.1"
                     /translation="MTPDFRSTLIDFIRREAKPIDKYGHQPRLYALTRQVGEGLAYDD
                     DVVFAAVWLHDLGVFAGHRPEDPTELARWDHVRYACEHAPRILAGTGFPTEKIPAVLA
                     AIREHQPQDGPTSTEATILRDADILEQLGAIGILRAVSKVGRDTRYPTFTDAIRVLQK
                     NLEALPAKIRLESTKKLAAPRIVALREFLDAVETEAQRNLL"
     gene            7775..8941
                     /gene="dprA"
                     /locus_tag="VFA02_12080"
     CDS             7775..8941
                     /gene="dprA"
                     /locus_tag="VFA02_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZU10631.1"
                     /translation="MTASSALSESISTERLAWLALALTPGLGPRRILSAIRQTQSADE
                     ILHLPLTGLESLNFPAQAVQFIADGQALQAANKEWEAMQKSGASFLTYNDAEYPERLQ
                     QIFDPPALLWIRGDASLLSRPSIAVVGTRHPTPYGAGMAEMLSRDLAARGIIILSGMA
                     RGVDTAAHKGALAAKGQTVAVWGTGIDVIYPKENKSLAEQIIAAGGAMISELPMGTFP
                     APQNFPKRNRIISGMSVGVLVVEAGEHSGTRVTARCALEQDRDVFAVPGNVTSKNAWG
                     PNTLIKQGAKLVATWEDVWEELPSQIRLEIESAWELASKPQPGASLFEEAPLPPAESR
                     VMEVLRHDEALQLDEIMEKLEPELSSSEVFTALFELELAGRIKQLPGKNYVKSF"
     gene            9059..11656
                     /gene="topA"
                     /locus_tag="VFA02_12085"
     CDS             9059..11656
                     /gene="topA"
                     /locus_tag="VFA02_12085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZU10632.1"
                     /translation="MSKSLVIVESPAKAKTINKYLGKDYTVEATIGHIKDLPKNEIGV
                     ELKHRTFEPDLIVIPGKEKVVDRLKKLAAKSESVYLAPDPDREGEAIAAHLAEELSKV
                     VKKNAIHRVTFNEITPKAVRDAFQHARKIDSHLVDAQTTRRVLDRIVGYQVSPLLWDK
                     VRRGLSAGRVQTVAVRLIVEREREIKAFNPVEYWTIDADLEAQKSTPFTARFVGIDGQ
                     RARVETVDAPALPDQKITDEVVAQLKKAAWSVRSVERKERRRNSAAPFTTSKLQQDAS
                     RQLGFNVKRTMGVAQRLYEGVDIGGETIGLITYMRTDSTRVAPEAIGAVRNWIKSNLG
                     DNYLPESPNAFKSKKDAQDAHEAIRPTNVDLHPDEIRKYLSDEQYKLYRLIWRRFVAS
                     QMTPAVFDQTTVDIVAKAGRRYDFRVTGSVLRFDGYLKIYEEAKDKKDEDDEALSNKL
                     PALNEGQPLTLRELKPEQHFTEPPPRYNEASLVKELEERGIGRPSTYSSIINTIQDRE
                     YVQKIGGRSGRFVPTEIGTVVNDLLVKNFPYIFDTKYTARLEEELDDIEDGREKWTDL
                     LKAFYGHFEEELKDASKHMENIKRMEKPTDEKCPQCGSPLVLKWGKFGSFYACSSYDK
                     KKKGSCDYTRENFEAKPNLALNDGQENEQEEEYCENCGRVMVLRNGPWGPFMACPGYN
                     EDPPCKTVRRLHQKQQQKPPEPIDEVCPKCGKQLVKRQGQYGEFISCSGYPKCKYIKQ
                     NTIGVKCPKCHEGEIVEKKARRGNIFYGCENYPKCDFTANYKPVDQKCPECGSPYLLE
                     KTLKSGVYLVCPNRKTTADEEEGTKKRRKKGEEEPAVTCTFSKRIGDAPEPERPRPET
                     HAPLVEQAV"
     gene            complement(11723..12175)
                     /locus_tag="VFA02_12090"
     CDS             complement(11723..12175)
                     /locus_tag="VFA02_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10633.1"
                     /translation="MIVFEGQMLLRQNISLPEQVHVRGNSLSEHWLALDESAVETDKK
                     VRSAEWHFFWLTHECQGWSLALDPQQALKAALRKAAEKIDRRYNAGEVVRITKRNFLG
                     LYFCKIELAARHIQQGPILGLTETVGIIAPALVPSEKAEIKRPVQVAA"
     gene            complement(12194..13003)
                     /locus_tag="VFA02_12095"
     CDS             complement(12194..13003)
                     /locus_tag="VFA02_12095"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZU10634.1"
                     /translation="MKFWREILCTGTILGLCVYAYSADTTGALKRGYPQASLVQAARE
                     ALDRDPALALVEVTISRGTATLSGSVDHYQDKLNAEAAVRSIPGIRNVEDNVMVTATP
                     VDDATLENQIEDRLHYGWSGDTPMLPDVHVSVANGVVTLSGTVKDPVYHAMALAIAGS
                     TDGVTQVLDHLQTIPDLASDDEVKLEISKLVHTTYPGVHAVLKDGIVTLMGSVPNDQQ
                     KKQLISKINGLYGVISVNDELVVGSEKRASEMREAAMAAPLYPGCPTIQQR"
     gene            complement(13100..13582)
                     /locus_tag="VFA02_12100"
     CDS             complement(13100..13582)
                     /locus_tag="VFA02_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZU10635.1"
                     /translation="MVLTRWQPFRELSSLQDRVNRLFSEFFPEMDGDNSSLIAPWFSP
                     RTDVYEEDDHVLLEMEIPGVKQEDLNITIENNVLTVSGERKFDERRKQDRYQRNERFY
                     GSFSRTFTLPATVDPDNIEAKYENGVLYLTMPKKADARPRQIKIGAPPQKQLTGSKAA
                     "
     gene            13815..14297
                     /locus_tag="VFA02_12105"
     CDS             13815..14297
                     /locus_tag="VFA02_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZU10636.1"
                     /translation="MSADDRVLRDNLIEFLRGGSAHIELHAALKDFPPAFYGAKPKGT
                     PYTAWQLLEHIRIALHDLLEFCTVPEYVAPEWPKDYWPEAAAPSSPEQWNASLRAIQE
                     DLAAFEKLAGGPVSNLYAQIPWGDGQTLLREILLAGDHTSYHLGQLVMLRKQLERSMS
                     "
     gene            complement(14362..14943)
                     /locus_tag="VFA02_12110"
     CDS             complement(14362..14943)
                     /locus_tag="VFA02_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZU10637.1"
                     /translation="MMRRGLWIAIAIVVVIVLAGLLVFGSYVSAKNQMVAKQEAIHAQ
                     WSQVDVVLQRRADLIPNLVETVKGYAKHEETVFSDIANARAALLNAHDPKDKIEANGR
                     LDSAIGRLLALSENYPNLKADQNFLALQDQLEGTENRIAVERRRYNQTLQDYNTFIRQ
                     FPNSIWAGIAGFQPDNDYFQASEASRQAPQVKF"
     gene            complement(15009..15761)
                     /locus_tag="VFA02_12115"
     CDS             complement(15009..15761)
                     /locus_tag="VFA02_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZU10638.1"
                     /translation="MLIFPLAAWTQAVKDLPRATDYVSDFANVLSPDTRQQLDQLCGE
                     VDHQANAQIAVVTIKSLEGQAIQDYAVELWDKWKIGRKDRGVLVLLAVQDHKYWIATG
                     YGVEGILTDAQAGVIGRQMVPYLRQGDYDDAVSLAVNQIAQMIAKDAGVTLQGLQQDR
                     VARQQHAKQLSLGQLIVFGIVIFLVIVFLARAGGSGLLGFLLGMFLGGGGRGGWGGGG
                     WGGGSGGDSGGGGGFGGWGGGSTGGGGAGGSW"
     gene            15873..>16381
                     /locus_tag="VFA02_12120"
     CDS             15873..>16381
                     /locus_tag="VFA02_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10639.1"
                     /translation="MLRRLLVFALTFTPLLTAHGTAQSSKDPLNYDEVEQVREYANQP
                     NERLKLYQKFIDQRVEAITKLANDRKAQNRSAELRARIEEFTSLVDELQDNLDTYDHD
                     HADIRKALKSLSVASVKWEPALHLPEANPLYDFDRKSAVEAAKSAVDQIKQVQNEQEK
                     YFIEHKDQAG"
BASE COUNT         3682 a         5034 c         4333 g         3332 t
ORIGIN      
        1 tgggaggcgt ccccaagcac gccgcgcatc atcatctgtg tccctttctg gtccagaatg
       61 ctccagatcg gctcgttgtc caccgccgac atctgctgca tcatcgaatc attcgacagc
      121 aggtttggag caagcccatc ccgggcatca agcgcatatt tgatggaatc gctcgaccca
      181 aacaccatcg tcgtcggatt cagaaagcag accagcatcc cgctgccacc catgggataa
      241 agcgcgttgt cgcgtatctt cgtcggcttc accttctttt tgcggaaatt ggcaagaatg
      301 tcctgcagag aaaactgtcc ctgcgccacg ccgatgatac gggtgtagtc cacactggga
      361 ctgctgcgga atgcggcaaa ggccagctca tcaatatcgt ggttatcgtt cagaccggaa
      421 gtcttcagcg cctgttccag ttccttcagc tcgggcggaa gaatgcgcgc tttcaggttc
      481 attgccgcgt cggaattctg catggcgcgg taatctacaa cgatcagttg ctgcacgtct
      541 ttaggaatgg cggcccgcgc atcttcgctc aattgcgcgg ccagggctgg cagtccggcc
      601 agaattccaa gacctgcggc cagtactgct ttcgacaaaa gcttcaaatc tctacctccg
      661 ggaacataag cccctgaata ccttatttta gaccggagcc ggccactcca aaggatgttg
      721 caggcggcgt tccagaatct tctcttcagg atgaaaccca tctaattgac gcacccactc
      781 gctctccggc tcaaaagccg cctccggaag cagcccaatc agctctccgc gcgctggctg
      841 cgtgcggtat ctggccgcct gccgcttgac tgccgcatag gccgacgcaa ccgaggtctg
      901 ccggaagtcc gtgatattca tcgagatctg cgccatcccg tgggccagca cgcccatggc
      961 cttcacgccg gaaagccctc cgctcgacgc ccgtatctcc tttgcaatcg cccgcgccgt
     1021 cgccacattg tccgtagcca gataaatatt gtaggcaatc agataacgcc gcgcccctac
     1081 cgccgaagcc cctgccgtcg gatgcaggtc cggcccgccc acatccggac gtcgcgaggc
     1141 gtccttgcgc acggcctcgc gcagcccctc gaactgtccc cgccggacat cttccaacag
     1201 cgcgcggtcg ggccgggcag ccgccgcctc gtaaaaatag accggcacgt ggtggcgctt
     1261 ccagatttcc atgcccgcct gccgcgccag cagcgcacac tgctccaacg aaatgttgcg
     1321 aatgggcaca aacggcacca cgtccgcggc cccgatacgg ggatggacac cctcctgtgc
     1381 cgtcagatca atcaattcag cagcccgccc ggctgcccgc accgccgctt ccacaatcgc
     1441 agcaggaggc cccgccacgg tcaccacaga ccggttatgg tcgctgtcca tagaccagtc
     1501 cagcagcaca acaccctcca cccgcatcgc cgaaagaata gacagcacct tgcgcgcatc
     1561 acgtccttca gaaaaattcg gcacacattc aatcagggcg tcgttcgtca tcttctcgtc
     1621 tgcttgtgtt ctgcttcagt gtaacgtgtg atgaccacgc cgttgtccta tctggccgcc
     1681 ttccgtaacc ggtcgcgaat cgcaattcca atcccttccg cctttggcac agggcaaagg
     1741 atgacttctg ccccggcctg atccagaaaa cgcagcccag caaacaagcg ttgagcaagc
     1801 tcttcggccc gctcccatct gccccagtca aaaaccgcca tatcttccgt ccggaggcgc
     1861 gccgcaaaca catccggcag catcaggcct gtcttcattc cctggtgcct tgcccgctct
     1921 acggcgtcca caaaagcagc gatctgtgcc tcgccctctc catccaccag gaccaatttc
     1981 gcctttggag cataatgccg cagacccact ccgggcgaag gcagggcctc cggaggcgtc
     2041 tcgtgcaatt cctccggctc acgaaatcca accgcctcca cgcccgccgc gcgaatctgc
     2101 tccagtgaaa ccactcccgg acggtacacc acgcacggcg tctcaaccgc atctattacc
     2161 gtagattcca gcccgtgcca ggtctccccc gcgtccagaa ccgcatcaat ccgtccatcc
     2221 aggtcctcaa ccacatgcgc ggcactcgtc ggactcgtcc gcccaaaacg gttcgcgctc
     2281 ggcgcggcga caggcacccc tgccctgcga atcagggcct gtgccaccgg atgctgcggc
     2341 atccgcacgc caattttgcg ccgccccgca gtcacctcgt ccgggacctc gtcctttttt
     2401 tccagaagca gcgtcagagg tcccggccaa aaccgctctg ccagccgatg cgctgcctcc
     2461 ggaacatttt tcgccacccg ccacagcatc tccatgtccg caacatgcac aatcaacggg
     2521 tcccacgatg gtctctgctt ggccgcaaag atcttcgcca cggccgtcgc atccagcgcg
     2581 ttcgccccca gcccatagac agtctccgta ggaaacgcga ccgtgcctcc ctcgcgcaaa
     2641 atggccgcag cctgctccag ggtggaccgc gcggcttctg aagcaatttc tccggaactg
     2701 actttcaggt gaatcgtacg cacaattaca gagtacaggt ctcggaaacc ctgaacgaaa
     2761 tggactatat aggtactttt taccggtcca tcgcggcccc tgcgccccgg ccccgcgcgt
     2821 tgtaccctgt atgcatgagc gccgtcgaga tcgaggtcaa attccgtgtt gccgatggcg
     2881 aggcactgga gcggaaactg ctttccatcg gcttccacaa agtaacaccg cgtaccttcg
     2941 agaagaacat acttttcgat accccggagc gctctctgcg tgcccaacaa tccattctcc
     3001 gcatccgcca atacggcgag cgatggatcg ttacccataa atgcctgccg caggaccacg
     3061 atgctgccgc ccgccacaag caccgcgtcg aaacggaaac cgaagtcagg gacggtgagg
     3121 ccatggccat tatcttcagc gagttgggat ataaaccagc ctttgcttac gaaaaatggc
     3181 gcaccgaata cgctgaccac accggtcatt gcgtgataga tgaaacaccg atcggcttat
     3241 ttgccgaact cgaaggcccg ggcgaatgga ttgactccat tggcaagaag ctgggcctgg
     3301 acccgtctga aatgatcacc ctcagctatg gtcgtctctt cgacgaatgg cgcaaagaga
     3361 ccggcagcac agcaacggac ctgacgtttg aggccattgc aaatcagaaa caggactgaa
     3421 ggccggaaaa ccggggactg acaactgaga actggcaacc ggcccccctc aagccttttc
     3481 attttctgcc gataagcacc tcagacaagt tctgaggtcc ctatgaattc ccgaaatttt
     3541 cgtaaatctg cttttggagt attgtggcgg ctggcccttg cgtttgcctg tctgctgatt
     3601 gcactgcctg gacatggtga agagcgccgc gtagaaaagc gcgtgtcccc ggtgtatccg
     3661 gagattgcca aacggatgcg cattggcggt gtggtgcatg tggaggccac ggttgctgca
     3721 gacggcagtg tgactgcggt caaggcagta tcagggaaca agatgctgac cccagcggcg
     3781 gaagaggcgg taaagcgctg gaagtttgcg cccggagacg gccagtccac tgtgggcgta
     3841 gacatcaatt ttgaagtgaa ttagatccat tcaatcctat aaagaaggaa gtagcgaggc
     3901 catgacattc acgcagtcgc gcctgaccaa caagctgcgc ctgagcacgg gcgtattaat
     3961 agtgagcatt ctgcttggct cctcggccgc ctatctcaag atgcggcagg ccagccagct
     4021 tgccgaaaat gtggcgtcgg agcagattcc ggcgctcaca gccgtgcgcg acctgcgcgt
     4081 tgcgctgctt gactcttcga atgcgctgaa ggcgtacatg ctttttggca tagacccgag
     4141 catggcctcg cgttacaagg cagtgctgga aaagagcaag gccgacgcaa acgatgcgat
     4201 ttcgcgcata ctggcaaagc gtcaggtgat tgatgccatg gccggatccg gacaggtgga
     4261 cagtctgctc aatgaatatc gcgcattcga gcaggggcag gaccatgtgg aggcgatggc
     4321 catcggccag ggcagcgatg caacgagcaa ggcctatgat cttctgcagg gcgaagtggc
     4381 caatcacgag gcggcgtgca gaaaagccgg ctcggctgtg gtttcagcca tgatgcaggc
     4441 caccgacgcc agcctgcatg agacggtgca ttccatgcgt ctgcaggcga tttatctctg
     4501 gctggcgatt ctgtttggcg gaatcattgg ctcctcgctg gctgagctca caattcgccg
     4561 agtggtgcgc tctgtcgtcc tcgtcgcaga tcgcgcgcag cagattgcgg aaggcgacct
     4621 gacaggcgag gccctgcata ccgactcgca ggacgaagtc ggcagcctgg caacctccat
     4681 caaccggatg caggacaatc tccgcgagat gatccgcacc atgatggaga tctcgaacac
     4741 cgtcaacagt gacgcagaaa agctcgcgca ctcggcatcg gagtccttcc gccgcacgaa
     4801 agagcaaagc ctccagacgc agcaggcggc cacggcgatg caggagatgt ccatcagcat
     4861 tgcggaagtt tccaggcact ctcagaacgc ggccgaaaac gcgaaagaag cctcgaaaat
     4921 cgcaaaagaa ggcggatcaa ccgtcgaaca gatgctcatc tccatgcagt ccattgccga
     4981 ctctgtgcgc aacaccgcct cgaccgtgca gcgcctgggc aaagaatctg agcagatcat
     5041 ccgcatcgtc aacgtcatcg aagaaattgc gcaaaaaacc aacctgcttg ccctcaacgc
     5101 cgcgatcgaa gccgcgcgtg ccggagaaca gggacgcggc tttgccgtcg ttgccggaga
     5161 ggtccgccgc ctggctgaaa gcactcgcaa cgccaccagc gaaatcgggc agatgattga
     5221 aggcatccag gtgcacacac gttctgccgt ggaagcgatg gatgcaggca cagccaccgt
     5281 caacgaaggc gtggaaacca ccacccgtgc gggcgaggca ttgcagcgca tcatccacat
     5341 ggccgaccag gtggacagca tgattgcaca gattgccgct gccgccatgc agcagtctga
     5401 agccgcacgc cagtcgagcg cgaacgtgga catcatcaac cgcctcggcg aagaaaacgc
     5461 cgcggccatt ccggaaagca acgccatcgt cacttctgtt caggccgggg ccaggcgcct
     5521 gcaggagcac atctcgcgct tcaagctgga agaaggcggg cggaccgtcc gcgcggcctt
     5581 tgcttcgcag ggatttgctc cgcagaccgc tgccgcttat ggagactaac gttcagcgcg
     5641 cgccagaaaa agagggcaga gtcaaaaagg ctctgccctc tttttctgca ccaggtccag
     5701 agaaaacccc acctgcctgt ctcatggtga gaaaaattcg cttcttagta cccacgcctt
     5761 gcttcgtccg catcttcagc caggtcgcgc agataagcaa agaaccccgg tgactgcccg
     5821 cgaaacaacc catccgctgt gaatgccgcc agcgctgccg tggctgcgcg ttttgtctct
     5881 gtgggccgga tgatggaaac caccggaaat ttcttgctca gaaaaaccgt cggctccggc
     5941 atgatgaggt ttgcctggag gttttcattc ccttcaaatg tgctgatgtc cggaaagtcc
     6001 accgtctttc cgctgaccgg atcgtgcacg cccgtcaatg ccggacagtt ccccgccttg
     6061 tcctgccatg tctgaaaatg cgcgatttcc gttccgccga tgctcagcag aatgcgcagc
     6121 acctccacgc ttttcacgcg ctgcgccagt tgcgcgtaaa gactcgtccc gccctgctca
     6181 ataaacgcaa agtggaaccc ggcggtgttg gcaataaact gcgtcacatc ggtgacccca
     6241 ttcggcgaag acgggtccag ttgcgcctca tcatcgtttc ggggaatggc cgtatgccgg
     6301 tctgttccga tcaccgcaat ggcattggga aaactgtctc cgaagtcggg gttcttctgc
     6361 ccgctgcggt accgcgtcca ccacgaggtg tccacggtca gctccatcag attggtgagg
     6421 cggttgattt gttgcgctcc tgttgccttg ctgctgggca gagtgcgaaa cggggtcagg
     6481 tctacagtat ctgcgccttt cgatttcaga tactcgttta tgaaggccgc atggctgaat
     6541 tcatcatcgg tattgtcggt aatatattgc ggcatgtcgg aatcgagatt tgtgagtgct
     6601 ttggtgtagg ctgtgtttcc ggtggtcagc cctttcagat agggggcttc gttcttttgt
     6661 actcctccca gttctgcgta ttgctgccac agatcagctt caagcgtttc agccgctgcc
     6721 agaaagcgaa ggatggctgc gtcgcctgga tctagtctgc cgccgcgttc ctctgcctcc
     6781 agaggaagat tgcctagaag ggcgcctgta gaaatggccg ctccggcaac tcccatgctt
     6841 ttaaggaaat tgcgccgttt gactgcgctt ttcattgggg gactctgttc catgctgcac
     6901 actcctgaaa aaaatttggt tttacctggc ccgtgtgtag cagcttcctc cagggcggaa
     6961 tgccgtgtgt catccatccg tcatccttgt gtcatctctt tgctactctg gaatcctgat
     7021 gacacccgat ttccgctcca ccctcataga tttcatccgc cgcgaagcaa aacccataga
     7081 caaatacggc caccagcccc gtctttatgc gttgacccgg caggtcggcg aaggacttgc
     7141 ctacgacgac gatgtggtct tcgccgcagt atggctgcat gatctcggtg tgtttgctgg
     7201 acaccggcct gaagacccaa ctgagcttgc gcgctgggac catgtgcgtt atgcctgcga
     7261 gcatgctccg cgcattcttg cgggcacagg atttcccact gagaaaattc cggcggtgct
     7321 ggccgccatc cgcgagcatc agccgcagga tgggccgacc agcacagaag cgacgattct
     7381 gcgtgatgcc gatattcttg aacagctcgg cgcgattggg attctgcgcg ccgtctcgaa
     7441 ggtaggccgt gacacacgct accccacctt cacggatgca ataagagtgt tgcagaaaaa
     7501 cctggaggcg ctgccggcga aaatccggtt ggaaagcact aaaaaactgg ccgcgccgcg
     7561 catcgtcgcg ttgcgggagt ttctggatgc agtggagaca gaagcccaga gaaatctgct
     7621 ttgataacag ctacgtttgt gataaagacc tgctgaaagc tgctccgctg gaaggagttt
     7681 tgttggtttc cccctaccct tccgtaactt gacttccctc cccggccccg ctactctccc
     7741 agcaaattcc caaaatagaa caggtacacg cgcattgact gcttcttctg ccttaagcga
     7801 atccatttct actgagcgcc ttgcatggct tgcgctggcg ctcactcccg gactcggccc
     7861 tcgccgcatt ctcagtgcca ttcgccagac gcaatcggcg gatgagattc tgcatctgcc
     7921 cctcaccgga ctcgaatcac tcaactttcc cgcgcaagcc gtgcagttca tcgcggacgg
     7981 gcaggcgctt caggcagcga acaaagaatg ggaagccatg cagaagtccg gcgcgtcatt
     8041 tcttacatac aacgacgcgg aatatcctga acgcctgcaa caaatcttcg acccacctgc
     8101 gcttctctgg attcgtggcg acgcatctct gctctccagg ccttctatcg ctgtcgttgg
     8161 aacgcggcat cctactccat atggggcggg catggcagag atgctctcac gcgacctggc
     8221 agcgcgcggc atcatcattc tcagcggaat ggcgcgcggt gtagacacgg cggcgcacaa
     8281 aggtgcgctt gccgccaaag gccagaccgt tgctgtttgg ggaacaggca ttgatgtcat
     8341 ctatccaaaa gaaaacaagt ctctcgccga gcagatcatc gcagccggag gcgcaatgat
     8401 ctccgagttg ccgatgggca cctttcctgc tccgcaaaat ttccctaaac gcaaccgcat
     8461 catcagcgga atgagtgtcg gcgtccttgt ggtcgaagca ggcgaacaca gcgggacgcg
     8521 cgtaacggcc cgctgcgcgc tcgaacagga ccgcgacgtc tttgccgtgc cgggcaatgt
     8581 gaccagcaaa aacgcatggg gccctaacac attgataaaa cagggcgcta agctcgttgc
     8641 cacgtgggaa gatgtctggg aagagcttcc ttcccagatt cgccttgaaa ttgaatctgc
     8701 ctgggagctt gcatcgaaac cacaacctgg tgcatcgtta tttgaggaag ctcccttgcc
     8761 gcccgctgaa tctcgtgtga tggaagtgct tcggcatgac gaagcattgc aactggatga
     8821 gatcatggag aagctggagc cagagctgag ttcgtcagaa gtgtttaccg cgctttttga
     8881 attggagctc gcaggacgca tcaaacaact tccgggtaaa aattacgtca aaagtttttg
     8941 attcccgcgt gagtgttgcc atgcaccgtt cactggtttt ttgcgtcttt aaaaatgtcg
     9001 ctctggattc tttgcggcaa ttcgtgatag cttccagaag cattggcaaa ggaaaacaat
     9061 gagtaaatcg ctcgtgatcg ttgagtctcc ggcaaaggca aagacgatca acaaatatct
     9121 aggcaaggac tacacggtcg aagcaaccat cggtcatatt aaggacttgc ctaaaaatga
     9181 aattggcgta gaactgaagc accgcacctt tgagccggac ctgattgtga ttcccggcaa
     9241 agagaaggtc gtggaccgcc tgaagaagct cgctgcaaaa tcggaatccg tctatctggc
     9301 gcctgaccct gaccgcgaag gcgaggccat tgccgcgcac ctggccgaag aactgagcaa
     9361 agttgtaaaa aagaacgcca tccaccgcgt cacgtttaac gaaatcacac cgaaggctgt
     9421 acgcgacgcc ttccagcacg cgcgcaagat tgactcgcac cttgtagacg cgcagaccac
     9481 gcgccgcgtc cttgaccgca ttgttggcta tcaggtttct cctttattgt gggacaaagt
     9541 ccgtcgcggc ctctctgccg gacgcgtgca gaccgtcgca gttcgcctca tcgtcgagcg
     9601 cgaacgcgaa atcaaggcct tcaatccggt cgaatactgg accattgatg cagatctgga
     9661 agcgcagaaa tccacgccat tcaccgcgcg ttttgtcggc atagatggcc agcgcgcccg
     9721 tgtggaaacc gttgatgcgc ctgcactgcc cgaccagaag atcacagatg aagttgtcgc
     9781 gcagttgaag aaggccgcat ggtcggtccg ctccgtcgag cgcaaggagc gcaggcgcaa
     9841 ctcggccgcg cccttcacca ccagcaaact ccagcaggat gcatcccgcc agctcggctt
     9901 taacgtaaag cgcaccatgg gcgtggccca gcgtttgtac gaaggcgtgg acattggcgg
     9961 cgaaaccatc ggtcttatca cctacatgcg tactgactcg acgcgtgttg cgcccgaagc
    10021 catcggtgct gttcgcaact ggatcaagtc aaacctcggc gacaactatt tgccggaatc
    10081 gccaaacgct ttcaagtcta aaaaagacgc gcaggatgcg cacgaggcga ttcgtcccac
    10141 caatgtagac ctgcatccgg atgaaattcg caaatacctg agcgatgagc agtacaaact
    10201 ctaccggctc atctggcggc gctttgtcgc ctcgcagatg acgcctgcgg tcttcgacca
    10261 gaccacagta gatattgttg caaaggccgg ccgcaggtat gacttccgtg tgaccgggtc
    10321 cgtcctcagg ttcgacggct atctgaaaat ctacgaagaa gcgaaggaca agaaagatga
    10381 ggacgacgag gcgctctcaa acaagctgcc tgcgctcaat gaaggccagc cgctcacgct
    10441 gcgcgagctt aaaccagagc agcactttac cgagccgcct ccacgctata acgaagcctc
    10501 gctggtaaag gaactggaag agcgcggcat cggccgtcca tccacgtact cgtccatcat
    10561 caacaccatt caggaccgcg agtatgtgca gaaaatcggc ggacgctcag gccgctttgt
    10621 gcctacggaa atcggcaccg tagtgaatga cctgctggtg aagaacttcc cctatatctt
    10681 cgacacaaag tacacggcgc gtctggaaga agaactggac gacatcgaag atggccgcga
    10741 aaagtggacc gacctgctca aggcctttta tggccacttt gaagaagagc tgaaagacgc
    10801 cagcaagcac atggagaaca tcaagcggat ggagaagccg acggacgaaa agtgtccgca
    10861 gtgcggctcg ccgctggtgc tcaagtgggg caagttcggg tccttctacg cgtgcagctc
    10921 ttacgacaaa aagaaaaaag gaagctgtga ctacacgcgc gaaaactttg aggccaaacc
    10981 gaatcttgct ctcaatgatg gccaggagaa cgagcaggag gaagaatact gcgagaactg
    11041 cggtcgcgtg atggtgctgc gcaacggtcc ctggggcccc ttcatggcct gtcctggcta
    11101 taacgaagat cctccgtgca agaccgtgcg ccgtctgcac cagaagcagc agcagaagcc
    11161 gcccgagcca attgacgaag tctgcccgaa gtgcggaaag caattggtca agcgccaggg
    11221 ccagtacgga gagttcatct cctgctccgg ttatccaaag tgcaagtaca ttaagcagaa
    11281 cacgattggt gtaaagtgcc cgaagtgtca tgaaggcgag atcgtcgaaa agaaggcgcg
    11341 ccgtggcaac atcttctacg gatgcgagaa ctacccgaag tgcgacttca cggcaaacta
    11401 caaacctgta gaccagaagt gcccggagtg cggttcgcct tatttgcttg agaaaacgct
    11461 caagtcgggt gtctatcttg tctgcccaaa ccgcaagacc accgctgatg aggaagaggg
    11521 cacaaagaag cgccgtaaga agggcgaaga agaaccggca gtcacctgca ctttctcgaa
    11581 gcgaatcggc gacgctcccg aaccggagcg gcccagacca gaaactcacg cacctctggt
    11641 agagcaggcc gtttaaaatt caagcagcag tacctcatgc tcccatcctt tgcagcagca
    11701 aggaatggga gcagatattg ttttacgcag ccacttgtac cggcctcttt atttctgctt
    11761 tttctgacgg tacaagggcc ggagcaatga tccccaccgt ttctgtcaga ccaaggattg
    11821 gtccctgctg aatgtggcga gcggccaatt caattttgca gaaatacagg cccagaaaat
    11881 tcctttttgt tatcctgacg acctcgccgg cattgtaacg cctgtcaatc ttctccgctg
    11941 ctttgcggag cgctgccttc aatgcctgtt gcggatccaa cgctaaagac caaccctggc
    12001 attcatgcgt cagccagaag aagtgccatt cggcagaacg cactttcttg tcagtctcaa
    12061 ctgcgctctc atccagagcg agccaatgct ccgataagga gttgcctcgc acatgtacct
    12121 gctccggcag agagatgttc tgtctcaaaa gcatttgccc ctcaaagaca atcatggccc
    12181 caaccctcct tacttatcgc tgctgtatgg ttggacatcc gggatacaga ggcgcagcca
    12241 ttgcggcctc gcgcatctcc gacgcccgct tctcactgcc cacaaccagc tcgtcattca
    12301 cgctgatgac cccatacagc ccattgattt tgctgatgag ctgctttttc tgctggtcat
    12361 tcgggacaga ccccatcaga gtgacgatgc cgtccttcag cactgcgtgc acccccggat
    12421 aggttgtgtg cacaagcttg ctgatctcca gcttcacttc atcatcgctg gccaggtccg
    12481 ggattgtctg caaatgatcc aatacttggg tgacgccatc cgtgcttccg gcaatcgcca
    12541 gtgccatagc gtggtacacc ggatctttta cggttccaga cagcgtcact acgccgttcg
    12601 ccactgatac atgcacgtcg ggcagcatgg gtgtgtcgcc agaccatccg taatgcaaac
    12661 ggtcctcaat ctgattttcc agcgtagcgt catctaccgg agtcgccgtg accatcacat
    12721 tgtcttccac gttgcgtatg cccgggatgg aacgcacggc agcttctgca ttcagcttgt
    12781 cctgataatg atccactgat ccggacaggg tcgctgttcc acgggatatc gtgacctcga
    12841 ccagagccag cgccggatcg cggtccaacg cctcgcgcgc ggcctgcacc aggctggcct
    12901 gcggatagcc cctttttaaa gcacctgtcg tgtctgctga gtacgcatag acgcacaagc
    12961 ctagtatggt gcctgtacac agaatttctc tccaaaactt catcgcagcc ctccttgcgc
    13021 taaagtctca aagaaaatgc cgggatggcc gcgtccggga ggacgccgca accatccaca
    13081 catggccgtc tgagaggatt caggcggcct tcgagccggt aagctgtttc tggggtggag
    13141 cgccaatttt gatctgacgc ggtctggcat cggccttctt cggcattgtc agatacagca
    13201 ctccattttc atatttggcc tcaatattgt cgggatccac cgtggccggc aaagtgaacg
    13261 tccgggagaa gctcccgtag aagcgctcgt tccgctgata gcggtcctgt ttgcggcgct
    13321 catcgaattt gcgttcaccg ctgaccgtca gtacgttgtt ctctatcgtg atattgaggt
    13381 cttcctgctt tacgccgggt atctccattt cgagcagaac gtggtcgtct tcttcataga
    13441 cgtccgtcct gggagagaac cacggagcta tcagagatga attgtcgccg tccatctccg
    13501 gaaagaattc actgaaaagc ctgttcacgc ggtcctgcag agaggaaagc tcgcggaacg
    13561 gctgccaacg ggtcagtacc attcgcatac ctcctcacat cagggatttc tgttttctac
    13621 ccggatggat gcagaagcgc aaaaagggtt gcatgaattt gagtgcgtta ttagcagatt
    13681 ttattatcat tccttaaggg ccattttgcg aagcaacaga cgtgcaaaag caaaaccagg
    13741 aaccacggac agaccctccg caatccttgc agcctccagc tctctggcgc atcccaacag
    13801 atggggaggg ctgcatgtct gctgatgacc gtgttcttcg tgacaacctg attgaattcc
    13861 tgcgtggcgg gagcgcacac attgagcttc atgcggcgct caaagacttc cctcccgctt
    13921 tctatggggc gaaacccaaa ggtacaccct acacagcatg gcagctcctg gagcacatcc
    13981 gaatcgccct gcatgacctg ctggagttct gcactgtccc cgaatatgtc gcacctgaat
    14041 ggccaaagga ctactggccg gaagccgcgg cgccttcatc tccagagcag tggaatgcct
    14101 ccctcagggc cattcaggaa gaccttgccg ccttcgagaa actcgccgga ggacccgtat
    14161 ccaatctcta cgcgcagatc ccatggggcg atggccagac gcttctgcgc gaaatcctgc
    14221 ttgccggcga ccacaccagc taccatctgg gccagttggt catgttgcgc aagcaactgg
    14281 aaagaagcat gtcctgacag ggcggcgcgc cttctcacct gacactcgga gcagcgtgtc
    14341 aggtggaaag accatagcct gctaaaactt cacctgcggc gcctgtcgtg aagcctcgct
    14401 ggcctggaag tagtcattat caggctggaa cccggctatg cccgcccaga tactgttcgg
    14461 gaactggcgg atgaaagtgt tgtagtcctg gagcgtctga ttatagcggc ggcgctcgac
    14521 cgcaatccgg ttctcggtgc cttcaagctg gtcctgtagc gccagaaaat tctggtcggc
    14581 cttcagattc ggataatttt ctgacaaggc caacagcctt ccaatggcag aatccagccg
    14641 tccattggcc tcaatcttgt ctttaggatc atgcgcattc aacagcgcgg cacgggcgtt
    14701 ggcaatgtct gaaaagacgg tctcctcgtg cttggcgtag cccttcaccg tctcaaccag
    14761 gttcggaatc aggtcagcgc gtcgctgcaa aaccacatct acctgcgacc actgcgcatg
    14821 gatcgcctcc tgtttggcaa ccatctggtt cttcgcgctc acatagcttc caaagaccag
    14881 cagtccggcc agtacaatca ccacgacaat cgcaattgca atccacagcc cgcgtctcat
    14941 catcgggaaa atctctcctt gctcaaaaaa ccgttgttcc atccagagag tatcatgcag
    15001 ccctgctctt accagctccc gccggcgcca ccgcccccgg tggaaccacc gccccaaccg
    15061 ccgaagccgc cacctccgcc actgtcacca ccactgccac caccccagcc gccaccgccc
    15121 caaccgccgc ggcctccgcc gcccagaaac atccccagca gaaacccaag caacccagat
    15181 ccgcccgcgc gcgccaggaa aacgatgacc agaaagatga caatcccaaa aacaatcaac
    15241 tgtcccaggc tgagctgttt tgcatgctgc tgtcgggcca cacggtcctg ctgcaaacct
    15301 tgcagcgtca cgcctgcatc tttggcaatc atctgcgcaa tctggtttac cgccagagaa
    15361 accgcgtcat cgtagtctcc ctgccgcaga tacggcacca tctgccgccc gatcacgcct
    15421 gcctgggcgt ccgtcaggat accttcgacg ccatatcccg tggcgatcca gtatttatgg
    15481 tcctggacag caagcagcac cagcacgcct ctgtctttcc ggccgatctt ccacttgtcc
    15541 cagagttcaa cagcatagtc ctggatggcc tgcccctcca gcgacttgat cgttaccacg
    15601 gcaatctgcg cgttcgcctg gtggtctact tcaccgcaaa gctggtccag ttgctgtctt
    15661 gtatcaggag aaagaacatt ggcaaaatca ctgacgtagt cggtcgcccg cggaaggtct
    15721 ttcaccgcct gcgtccatgc ggccaaggga aaaatcaaca ccaggagcgc aagcagcttc
    15781 cgagctgaat gtaaaagcat cgaaacctat tctacccgcc actgtgcaaa acccatcgtg
    15841 aacatctaaa cttggcagcg aaggaggccg ttatgctccg ccgtcttctc gtctttgctc
    15901 ttaccttcac tcccctgctg accgctcatg gaaccgcaca atcgtcaaaa gacccgctca
    15961 actacgacga agtagaacag gtccgcgagt acgccaacca gcccaacgag cgtcttaagc
    16021 tgtaccagaa atttatcgat cagcgcgtcg aagccatcac aaagctggca aacgacagga
    16081 aagcgcaaaa ccgctccgcc gagcttcgcg cccgcatcga agaattcact tcgctcgtgg
    16141 acgagttaca ggacaacctc gacacctacg accacgacca tgccgatatc cgcaaggcgc
    16201 tgaagtctct ttccgtcgca agcgtaaagt gggagccggc cctgcacctg cctgaagcca
    16261 acccccttta tgacttcgac cgcaaatccg ccgtcgaggc cgcaaagagc gccgtagacc
    16321 agatcaaaca ggtccagaac gagcaggaaa aatacttcat cgaacacaaa gaccaggccg
    16381 g
//
LOCUS       DASRBV010000328         9100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_428793, whole genome shotgun sequence.
ACCESSION   DASRBV010000328 DASRBV010000000
VERSION     DASRBV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428793"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1294..2349
                     /locus_tag="VFA02_12125"
     CDS             1294..2349
                     /locus_tag="VFA02_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZU10640.1"
                     /translation="MADTIRAGVIGYGLAGRIFHTAVIAATPGLELAAVVQRTGDEAA
                     KAYPGARLYRSIQELLEDASIRLIVVATPSGSHFQVAEQCLLADRDVVVDKPFTLTSD
                     EAGRLVRLSRERRRLISVYQNRRWDGDFKTVQKMIASGKLGRLVTFESHFDRFRPHPR
                     REVWRENGGPGGGTLYDLGSHLIDQALTLFGKPDHISASVRVERENAVVDDAFDITLF
                     YPKLTAYLRATNLACTPGARFTLHGTQGSFVKFGLDPQEEALKNGATFHTPGFGHDPE
                     KDWGTLYLEKAVPQKIETEVGDYRGYYASVRDALLGRQPLAVTAEDGWRTARIIELAR
                     QSSKEGRVLSVDFTGKP"
     gene            2738..4396
                     /locus_tag="VFA02_12130"
     CDS             2738..4396
                     /locus_tag="VFA02_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10641.1"
                     /translation="MAARWKPWLRLMGQAEAVIRKFSERPVVCFFGIYVTAIVIRLAL
                     LPVEPVPRPLLHDEFSYLLAADTFVHERLTNPQPPVPAAFETFHVNVWPTYQSMYLPG
                     TGLLLALGQLLGSPWIAVLLTSALFCAMVYWAISGWLPRPYGLAAALLALAVCFNWNR
                     WFDNYFCLGFMGTAGALVMGSVPRIFHRRSAASTVPLGIGLCILMLTRPYEGCIVSLP
                     AVCVLMWALLRHTGWKQVARLASVPLLMLFFAFGWLCYYNWRGTGHALLFPYVLNYRQ
                     YHITGPFIFSHLRPLPVYHHESMQKFYTHWEMNTYRMVTRSPFQFFRLKAKSYYHFYL
                     LGNGLVLLFGCIAAFSKRGLQLALALAILSFSVEVLLMAWYPYPQYAAPSAALFFTLI
                     AVGLFTMRRMQFRWCTGANLVRGWVLAQLLIAGNIFYKHWMKSPVPPEPRYASVERQK
                     VEKLILEHPGKHLCLVQYSLDHPWVQEWVYNGADLNSQRIIWARSMDPDDDRKLIQAF
                     PNRDVWLLRPDDPAKNLMAYSPDIAYPNPNRSVLARAVPPPPPF"
     gene            complement(4393..4770)
                     /locus_tag="VFA02_12135"
     CDS             complement(4393..4770)
                     /locus_tag="VFA02_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZU10642.1"
                     /translation="MIQLKRAYDPASPHDGRRFLVERLWPRGIRKEDLLLDDWIKDAA
                     PSTELRKWFHHDPEKWKEFQRRYFTELDSRPEVLAPILKAARHGCVTLIFSSHDAEHN
                     NAVALKSYLESHSAKAGRKSIKA"
     gene            complement(4856..7228)
                     /locus_tag="VFA02_12140"
     CDS             complement(4856..7228)
                     /locus_tag="VFA02_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZU10643.1"
                     /translation="MIRKIVLLFLCSAVSLFAWTQSAEQRVVLERGPSTIVLEPYAPN
                     IIRVTLSLKKDAALAPPGYGMIASPSAAGWTHEKKDVDEVYRSSSLVVTVKGWGRGKY
                     AIPQSVMDPGKYFSGSTPGADIVFSAPDGKKLAEMDGWSMSVPNHKDGNSQILYDRRP
                     TDPDFYQVGASFTSPDDEHYYGLGQNQEGFLDHRGHVVHCWHDYNAPGGQSVCVPFMV
                     TNYGYGVVWDNPSKTTIVPGLNEHTTWTSEVGDRVSFFLIAGSTVDEIYAGYRLLTGA
                     TPMLPKAAYGYIQCKQRYRSQDEVLAVAKGYRERHLPADVIVVDWFYYTKMGQMDFIP
                     SLWPDPSAMNRQLHQMGFQTMISVWPRFEPGSRYYDFLREKGWFEHLADGTPTNGLPY
                     DKAGSDIDTTNPDAAHWYWERIRENILSQGFDSIWADETEPDLPPNGSYLHIGPGTEY
                     FNVYPLFHTAAIYNGFRRDTQHRALVLSRDAYLGAQRNGAIFWSSDITATWDAFKRQI
                     PTGLDFAASGLTYWSNDTGGWQPLPQVHHPEHPPLLDPSDARDNVYDYDDYPELYTRW
                     FEYASFLPIFRTHGSRRYNEVWSYGKQAEPVLEKYLKLRYELLPYIYSLGYKTYKTGA
                     PYMRALFMDFPNDPNVADVRDEYMFGPAFLVAPVTEQGATSRKVYLPAGTDWYNYWTN
                     KRVKGGQTITVDAPIDTLPLFVRAGSIIPLGSYIESTAQPQKIAKISIYPGADADFTL
                     FQDDGNTYAYEKGDAQITHLHWNDAAQQLTHTGAKAWSGPDSQVISVVRP"
     gene            7601..8596
                     /locus_tag="VFA02_12145"
     CDS             7601..8596
                     /locus_tag="VFA02_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10644.1"
                     /translation="MKRIRTSLRALVLLVAAVTFAQKNTPPPNAGKATCVHPTKLYVE
                     ADESASQVATIQPGREMVIAEHSGKWLRVFANTDVSIVNEQDVPVFGNQTNTPPVSGW
                     MIDKGIVTSNTPNGDEILFGAAATMELQASEPHPPPGAALSARGLYRMVVELFPQSSR
                     VPEAMWRAADIRWQLQKADAFSLPSAHEKEAYLREQIDESEMKKIIKLYPHSRWAALA
                     AFDMIDNKICGDWQGSEKCPEKEADFFVKYADDYPDSPKAPQALYQAAWRMASAGDMY
                     QADNDEKKAGDARAKAKELSARLQSKYGQSDYAARAAGLVYMMDQKIPIYGSQEE"
     gene            complement(8611..8877)
                     /locus_tag="VFA02_12150"
     CDS             complement(8611..8877)
                     /locus_tag="VFA02_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10645.1"
                     /translation="MRIIRPVEWPDGITLRHREYLDGLIEDWSQTPPSQLSLLLDQLS
                     ELSTGPLRSAGHGQCASGQIDAVTAEFFASAKSHPESVLQQDQP"
BASE COUNT         2044 a         2423 c         2418 g         2215 t
ORIGIN      
        1 gcagggcgaa attcgcgtgc cggattctcc gggacgcggg tttgccgtaa ggactgacct
       61 gatcgaaaaa ctgaccgtgc gtaaggaaga ggtacgggca ctggcgcttg cgtgacacag
      121 gacgcctcag agcggttccg gagcccaggc gagaggcaaa ttttgtttca tttttcgcgc
      181 gcaatcttcc gcgaaattat ccattccagg ggtgggaaac ggtcctggca aaaccctgta
      241 tcgccaagat tcttcctccc ttcggccgtc agaatgacgg acttgtgcat ctacgaaaag
      301 tgctaaattc ctgaaaatat ttgagttgtc cccaatagag cgaggctgtg tatcgtaggg
      361 gggtaccccc ctgttttgta cgggaatcct ttgttttcaa taagttacaa gtgagtgttt
      421 gtacggagat cctttatttt caatagctta gttagcaaag aagcggccag cagtcagcgc
      481 ctgcttcgca ggctgtcagc gagagacttc tcagtttccc ggcttccata gataagctgg
      541 gtatccacaa aaaggtcagg ggcgaaagct taagctaaaa agcgggtggc tgaaatccgg
      601 gtggctaaaa aagcggtcag caaagaagcg ggtagctgag aagcggtcag cagtcagcgc
      661 ctgcttcgca ggctgtcagc ccggagattc ttcctccctt agttaggctc tcttttccgg
      721 ggatgtgttc ctggttcctt cggcttccca tagataacct aggtatatat aaaagtcagg
      781 actaattttt ttgtaaattc ttgaatctgt gatagttagt aaagtttagg ttctcatttt
      841 ttgactcatt tttataccta gattgtctac tgatggtcaa gaaaatggcg accggctttt
      901 ctcctgtatg taattgaaaa taaatttgtt ttcgatgttc ttgtatttca tttttcggcc
      961 tcctctgctt ccctagactg tctacttatc gctaagattc ttttcttccc ttcggtcgtc
     1021 agaatgatgg tttattctct agaatgtcta ctttatgcgc ttgagcacgt cgtcaggcat
     1081 aaatgattga aaataaatta ttttaagcac ttttgcctct cgctttcaac ggatttttga
     1141 tacctggatt ctctaccgat ggctgggttc tgacttttac cctttttttc aggatatcag
     1201 tttgaacagg ggaatccgga caatacaggt ttctatgcaa gttattgaaa tataatggct
     1261 tgtaccgatt tattactttc gtaaggattg acaatggcag acacaattcg agcgggcgtt
     1321 atcggttatg gactcgcagg gcgaattttt catacagccg tgattgcagc cactccgggg
     1381 cttgaactag cggcggtcgt gcagcgcact ggagatgaag ccgcaaaagc ctacccggga
     1441 gccaggctgt accgctccat ccaagagctt ctggaagatg ccagcattcg cctcatcgtt
     1501 gtggctacgc ccagcggctc tcattttcag gtggcggagc aatgtctgct tgcggaccgc
     1561 gacgttgttg tagacaagcc tttcacgctt acatcagacg aagcagggcg ccttgttcgc
     1621 ctgtcacggg agcggagacg cctcatctct gtctaccaga accggcgatg ggacggtgat
     1681 tttaagaccg ttcaaaagat gattgccagc ggaaaactcg gacgtctggt gacgtttgaa
     1741 tcgcatttcg accgcttccg tccccatccc cggcgcgaag tctggcgcga aaatggaggc
     1801 ccgggcggcg gcacactcta tgatctgggt tcgcatttaa tagaccaggc gctcaccctg
     1861 ttcggcaaac cagaccacat ctccgcaagc gtccgcgtag aacgggaaaa cgccgtggta
     1921 gacgatgcct tcgatatcac gctgttttat ccaaagctga ccgcatatct gcgcgcaaca
     1981 aacctggcat gcactccagg ggcgcgcttt acgttacatg gcacccaggg cagcttcgtc
     2041 aaattcggcc ttgacccgca ggaagaagcg ctcaagaacg gcgcgacctt tcacacgccg
     2101 ggcttcggtc atgatccgga gaaagactgg ggcactttgt atctggaaaa agcagtaccg
     2161 cagaagatag aaaccgaagt tggggactat cgcggatatt atgccagcgt gcgcgatgcc
     2221 cttctgggaa ggcagcctct ggccgtcacc gcagaagatg gatggcgcac cgcccgcatt
     2281 atcgaactgg cccgccaaag cagcaaagaa ggccgcgttc tgtccgttga cttcaccggc
     2341 aagccttaaa caaggcagcg cttatccggg taaacacttc agccactttc cgcgtccatt
     2401 agtacaggtg cgcattatgc gtgttcacag attctgtaca ctccatgcga gttagctgga
     2461 cagcacaggg caaaccatct gtcagaacgc catagcggac tgtacgtatt gctccggcgc
     2521 acttggggca ttttcacaga tttgtgcact ccatttgcac tgtgcatggg tcgcttatca
     2581 aagtttcctt aacggtagag gcggaccatc tgtgaaaacg ctgtaaagag actgcaaatc
     2641 agttcgtcag cttcttcgcc ggagaaaatt gcaccggatg gtacagtcgt cactgcacta
     2701 tcttcttatt gaagtgttag tcatttgcgt cgcttgtttg gcggcacgct ggaagccgtg
     2761 gctgcgcctg atggggcagg cggaggcggt catcaggaag ttcagcgagc gcccggtagt
     2821 ttgttttttc ggaatttatg tcaccgccat cgtcattcgt ctcgcgcttc tgcctgtgga
     2881 gccggtcccc cgtccgctgc ttcacgacga gttcagctat cttctggcgg cagacacctt
     2941 cgtccacgaa cgacttacaa atccgcagcc tcctgtgcct gccgcttttg agaccttcca
     3001 cgtgaacgtg tggccaacct atcagtccat gtacctgcca ggcacaggtc ttctgctcgc
     3061 gttgggacaa cttctcggct ctccgtggat cgcagtgctg ctcacctctg ctctgttctg
     3121 cgccatggtc tattgggcca tctcgggttg gctgccgcgc ccttatgggc tggcggctgc
     3181 actgctcgcg ctcgccgtgt gctttaactg gaaccgctgg ttcgacaatt atttttgcct
     3241 cggctttatg ggcaccgcag gagcactcgt catgggcagt gttccgcgca tttttcatcg
     3301 caggagtgct gcatccactg tcccacttgg cattggcctt tgtatcctga tgctcacgcg
     3361 accttacgaa ggctgtatcg tttcgctacc cgcagtgtgt gtattaatgt gggctctgct
     3421 acgccatacc ggatggaagc aggtcgcaag gctggcctct gtacctctgc ttatgctctt
     3481 cttcgctttc ggatggcttt gctattacaa ctggcgtggc acaggtcatg cgctgctttt
     3541 tccatatgtg ctgaattaca ggcagtacca cattacgggg ccctttatct tttcacatct
     3601 tcgcccctta cccgtctacc accacgaatc catgcagaag ttctataccc attgggagat
     3661 gaatacctat cgtatggtca cccgttctcc ctttcaattt ttccgtctca aggccaagag
     3721 ctactatcac ttctaccttt tgggaaacgg gctggttttg ctgtttggtt gtatagccgc
     3781 gttcagcaag agaggtctgc agctagcgct ggcgctggcc atactcagct tttctgtcga
     3841 agtgctcttg atggcctggt atccgtatcc gcaatatgcc gcgccatcgg ctgctctctt
     3901 tttcacgctg attgcggtgg gattgttcac catgcgcaga atgcagtttc gctggtgcac
     3961 tggcgcaaat cttgtgcgcg gatgggtgct ggcgcagctt ctgattgcag gaaatatttt
     4021 ctataaacac tggatgaaaa gccctgtgcc gcctgaacct cgctacgctt cggttgagcg
     4081 ccagaaagtg gagaagctga ttctggaaca cccaggcaaa catctttgcc tggtgcagta
     4141 ctcgctcgat catccctggg tgcaggagtg ggtctacaac ggagcagacc tgaacagcca
     4201 gcgcatcatc tgggcgcgaa gcatggatcc ggacgacgac cgcaaactca tccaggcgtt
     4261 ccccaatcgc gatgtgtggc tgctcagacc agatgatccg gcgaaaaatc tgatggcgta
     4321 ctcgccggac atagcctatc ccaacccaaa ccgcagcgtt ctggccaggg ccgttccgcc
     4381 gcccccacca ttttaggcct tgatggattt ccggccagct ttggctgaat gcgattcaag
     4441 gtagctcttc agcgcaaccg cgttgttatg ctctgcgtca tgtgagctga agataagcgt
     4501 cacacatcca tgccgcgcgg ccttaagaat tggcgccagc acttcaggcc gtgaatccag
     4561 ttcggtgaaa taccgcctct gaaattcctt ccacttttct ggatcatgat gaaaccactt
     4621 tctcagctct gtactcggcg ccgcatcttt gatccagtca tccaagagca ggtcttcttt
     4681 cctgattccg cgcggccaca gcctttctac cagaaagcgc ctgccatcgt gaggacttgc
     4741 cggatcatat gcgcgcttca gttgaatcat ggtcctgcct gcaaaagatg cagcatgcga
     4801 ggttatggtt tgtctgaagc ttgctgcatc atcaagcaat ttcagactca ggacgctaag
     4861 gcctgactac agaaatcacc tgggaatcag gcccgctcca cgcttttgcg cctgtatgcg
     4921 tcagttgctg cgctgcatcg ttccaatgaa gatgcgtaat ttgcgcatca cctttttcat
     4981 aagcgtaagt gttgccgtca tcctggaaaa gcgtgaagtc agcatccgca ccgggataga
     5041 tgctgatttt cgcaatcttt tgcggctgcg cggtgctttc aatgtaggaa ccgagaggaa
     5101 taatggaacc agcacgaaca aagagcggca gcgtatcaat gggcgcgtcc actgtaatgg
     5161 tctggccgcc cttcacacgc ttgtttgtcc aatagttata ccagtctgtt ccggcaggca
     5221 gatagacctt gcggctggtg gccccctgct cggttaccgg ggccactaag aacgccggac
     5281 cgaacatata ttcgtcacga acatccgcta cattcgggtc gtttggaaaa tccatgaaca
     5341 gggcgcgcat gtagggtgct ccggttttgt aagttttgta tccaagtgag tagatgtaag
     5401 gcagaagttc gtatcgaagc ttcagatatt tttccaggac cggctctgcc tgctttccat
     5461 aggaccagac ctcgttgtaa cggcggcttc cgtgtgtgcg aaagatgggc agaaaactgg
     5521 catactcaaa ccaccttgta tacagctccg gataatcgtc atagtcatat acgttgtcgc
     5581 gcgcatctga agggtcaagc agcggtggat gctcaggatg atgcacttgc ggcaacggct
     5641 gccatccgcc tgtatcgttg ctccagtagg tcaggcccga cgcagcaaaa tcgaggccgg
     5701 ttggaatctg gcgtttgaat gcatcccagg tggccgtgat gtctgacgac cagaaaatgg
     5761 cgccattgcg ctgcgccccc aggtaagcat cgcgcgagag cacaagggcg cgatgctggg
     5821 tgtctctgcg gaagccatta tagatagcag ccgtgtgaaa gagcggataa acattgaaat
     5881 actctgtgcc gggaccaatg tgcaggtagc tgccatttgg aggcaggtcg ggttcggttt
     5941 catcggccca aatggagtcg aagccttggc tgagaatatt ttccctgatg cgctcccaat
     6001 accaatgggc cgcgtcagga ttcgtggtgt ctatgtcaga gccagcttta tcgtaaggca
     6061 ggccgttggt tggagtgcca tccgccagat gctcgaacca tcccttttcg cgcaggaaat
     6121 cgtagtagcg cgagcccggc tcgaagcgcg gccagacgct gatcatcgtt tgaaagccca
     6181 tctgatgaag ctgcctgttc atggccgatg gatcgggcca caggctgggg atgaaatcca
     6241 tttgccccat tttggtgtag tagaaccaat ccaccacaat tacatcggcg ggaaggtgcc
     6301 gctcacgata acctttggcc accgccagga cctcgtcctg cgagcggtaa cgctgcttgc
     6361 attggatgta tccataggcc gccttcggca acatgggagt cgcgccagtc agcaggcggt
     6421 atccggcata aatttcatcc acggtgctgc cggcaatcaa aaagaatgaa acacggtcgc
     6481 cgacttccga ggtccacgta gtatgctcgt tcaatcccgg aacaatggtg gttttggaag
     6541 ggttgtccca gacaacgcca tacccataat ttgtaaccat gaaaggcaca catacactct
     6601 gccctccggg cgcattgtag tcatgccagc aatggacaac atgcccgcga tggtcgagaa
     6661 agccctcctg gttctgaccc agtccgtaat agtgctcatc atcgggcgaa gtgaagctcg
     6721 cgcctacctg ataaaaatcc ggatcggttg gccggcggtc atagaggatt tgcgaattgc
     6781 cgtccttatg gtttggcacg gacatggacc agccatccat ttcggcgagc ttttttccat
     6841 cgggagcaga aaagacaata tcagcgcctg gagttgagcc gctgaagtac ttgcctgggt
     6901 ccatgactga ctgcggtatc gcgtattttc cgcggcccca cccttttacc gtcaccacca
     6961 ggctggatga ccggtatacc tcatccacgt ccttcttctc atgggtccag cccgcagcag
     7021 agggagaagc aatcattcca tatccgggag gggcgagcgc cgcgtctttt ttcaggctga
     7081 gcgtaacgcg aatgatgttc ggagcatatg gctcaagcac aattgtggac ggaccgcgct
     7141 caaggacgac acgctgttcc gcggattgtg tccaggcaaa aagagagact gctgagcaga
     7201 gaaaaagcaa aactatctta cgtatcattg ggcgcgctgc tccttgctga tgtgcgcccc
     7261 cattctaaat cgttttatta gtaaatgtct tttcttgccg gagttttgct gtttcctggg
     7321 gcgcctgcgt ggccctcgca aattaaccgc ggcgcaattt tcgcgcagat gtgcccattc
     7381 cgaaactgca aaggtgacca gcaaaagaaa aagactggac cggaccgcaa aatacttcat
     7441 gactgcgcct cgcaaaacgt tctattgttc ttgaaaaatc tggaccggaa tgaaaaaggc
     7501 ccacgaactg tctttgtggg cctttatcat tgttcaaccg atgatgagag caccatcagg
     7561 attgcttaaa ctattcgcag tgttataccg tccaatgaac gtgaaacgga ttcgaacttc
     7621 tctgcgcgct ttggttttac tggttgccgc ggtaactttt gcccagaaga acacgccccc
     7681 tccgaatgca ggcaaagcca cgtgtgtgca tcctacaaag ctctatgttg aggcagatga
     7741 gtccgcttct caggtagcaa ccatccagcc cggacgcgag atggtgattg ccgaacacag
     7801 cggaaaatgg ctgcgtgtct tcgcgaatac ggatgtatct atcgtgaatg agcaggatgt
     7861 gccagtcttc ggcaaccaga ccaatacgcc gcctgtttcc ggatggatga ttgacaaagg
     7921 catcgtaacg tcgaacactc ccaatggaga cgaaattcta tttggcgctg ccgccacaat
     7981 ggaattgcag gcaagcgagc cgcatcctcc gccgggagcg gcattgagtg cgcgcggact
     8041 ctaccggatg gtggtcgagc ttttcccgca gtcctcacgt gttcccgaag cgatgtggcg
     8101 tgccgccgac attcgctggc agctacaaaa ggccgatgct ttttctttac cgtccgcaca
     8161 tgagaaagaa gcttatctgc gcgagcagat tgatgaaagc gagatgaaga agatcatcaa
     8221 gctgtaccca cactcgcgct gggcagctct ggccgccttc gacatgattg acaacaaaat
     8281 ctgcggtgac tggcagggat cggaaaagtg tccggaaaaa gaggcggact ttttcgtcaa
     8341 gtatgcggac gactatccgg actcgccaaa ggcaccacag gcgctgtatc aggcggcatg
     8401 gcgtatggca tccgccggag acatgtacca ggccgacaac gacgagaaaa aagccgggga
     8461 tgcgcgagca aaggccaaag aattgtccgc gcgattgcaa agcaaatacg gccagagcga
     8521 ctacgccgcg cgcgcagcag ggctggtcta tatgatggac cagaagatcc cgatctacgg
     8581 ctctcaggag gagtgaagca gtcaaatcac ttacggctgg tcctgttgca gaacagattc
     8641 tggatgggat tttgcactcg cgaagaattc agccgtaacg gcatctatct gcccggaagc
     8701 gcattggcca tgcccggcgg aacgcagcgg gcctgtcgaa agctctgaca attggtccag
     8761 aagcagtgat agctgcgagg gaggtgtctg actccagtcc tctatcaggc cgtcaagata
     8821 ttcacgatgc cgcaaggtga tcccatcggg ccattccaca ggccggatga tacgcaaatg
     8881 tcctctgctt tttcgacccc aggggtaaat atggaccacg gccaagcaag gacattccgt
     8941 ctcgcacaat ggtgccggca gatatttgag caatacaaac cggcaccgca tctgcgacat
     9001 gcagaaagta taaaacccaa tcgcctgcct gttcccggaa taaatttcac cgcagtttta
     9061 ttttgctcca catctggcgc acatatttct cagcgtgctg
//
LOCUS       DASRBV010000329        21304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_81911,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000329 DASRBV010000000
VERSION     DASRBV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 21304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21304
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81911"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFA02_12155"
     CDS             complement(<1..290)
                     /locus_tag="VFA02_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZU10646.1"
                     /translation="MKLHVRGILFDMDGVLVSSLGSVERSWQKWAAKRGVDPAIAIRT
                     AHGRRAIETVRLLRPDLNDVEELNLIEQIEIEDKEDIQVLKGVCRILDALPQ"
     gene            complement(426..644)
                     /gene="iscX"
                     /locus_tag="VFA02_12160"
     CDS             complement(426..644)
                     /gene="iscX"
                     /locus_tag="VFA02_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HZU10647.1"
                     /translation="MAREIYWTDAEEIGIQLYEKFPDVDPLTVRFTDLHKYVTELEGF
                     ADDPAKSNEPKLEAIQMAWYEEFKEGAE"
     gene            complement(644..1081)
                     /locus_tag="VFA02_12165"
     CDS             complement(644..1081)
                     /locus_tag="VFA02_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU10648.1"
                     /translation="MSEGKTNHNIDLSTPPAPNMVRVTFQPEGKTVEFEFGTMPYDHH
                     GKPMSFLDVAENYGIFLDHACGGACACTTCHVWIKEGAEGLSEADDDELDRLDMAADL
                     QLNSRLGCQCVITRPGNYVVEIPKWNRNYVSEGKPLALAEEKK"
     gene            complement(1138..3081)
                     /gene="hscA"
                     /locus_tag="VFA02_12170"
     CDS             complement(1138..3081)
                     /gene="hscA"
                     /locus_tag="VFA02_12170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HZU10649.1"
                     /translation="MSENKERIVGIDLGTTNSLVAFMQGEKPVVIPGEDGSNLVPSVV
                     ALDSANGQRRFIVGNAARAHLVRTPERVVYSAKRLMGRGIEDVQEELRLFPFRLAAGL
                     QPGEVLRIRLGEEEFTPPEISAYVLRQLKRNAERYFGAPVTKAVITVPAYFNDAQRQA
                     TKDAGRMAGLEVLRLVNEPTAAALAYGLDRNKEGIVAVYDLGGGTFDISILKLHEGIF
                     EVIATNGDTHLGGDDIDNLLIAIALEDIKGELGLDLSSSPEAVQAIRKAVIEAKIELS
                     SSEAARISVDLPGGHKYQRMLPRAAFEEIIRPILAKTITPCSQALKDAGVTPEQVDEV
                     VLVGGSTRIPAVRQLTDEVFHLSARGKKPHTELNPDEVVALGAAVQANILAGGSKATE
                     DMLLLDVTPLSLGIEALGGVVAKIIQRNSTIPASATEHFTTGVDGQTNVAIHVVQGER
                     ELAKDCRSLARFDLKGIPPMSAGLPRIEVKFLIDANGILHVSAREQRSGKEAEIEVKP
                     AYGLTDEQVETMILDSFDHAEEDFYQRQLIEARNEADTILRAVEKAPENPAWQQLTDE
                     ERAQIALLKEELSVLKQEDDLKALRAGTDALDKATRRFAELMMDAAVSSAIKGETMDS
                     AGNKLGEGPTTPHPFAPAEITKG"
     gene            complement(3078..3497)
                     /locus_tag="VFA02_12175"
     CDS             complement(3078..3497)
                     /locus_tag="VFA02_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZU10650.1"
                     /translation="MPFVRISVSEKMPVEKRRQLPKAVYDAMRASINIPENDLFVALH
                     AHGEGELIADPTFMGMQRTDGFTVVHITLRRGRTIEMKQKLYSEIVRLAQERAGIAPD
                     DVMIVLSENDLADWSFGKGEAQYVLNPPMQQKPEVLV"
     gene            complement(3592..4302)
                     /gene="hscB"
                     /locus_tag="VFA02_12180"
     CDS             complement(3592..4302)
                     /gene="hscB"
                     /locus_tag="VFA02_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HZU10651.1"
                     /translation="MQTIEQQSVKTACWSCGSEMSSGRPFCDTCGKVQAPVSSMTYFD
                     VFGLPRKLALDLSALERAFYKLSRKLHPDVYAQACAKEQQWSLEQTSLLNDAYRTLKN
                     PISRTEYLLRLEGVEMEQDKAAANGEKEPRVPADLLEEVFELNMQLEEMRMNQKMGED
                     DAQLRGDLEKAKAQFEGQLADADTQLQSLWEKWDAALDARDNASLDDAKDRMVALLDR
                     RRYVRNLVRDVNEALELK"
     gene            complement(4369..4752)
                     /locus_tag="VFA02_12185"
     CDS             complement(4369..4752)
                     /locus_tag="VFA02_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZU10652.1"
                     /translation="MSTQLNVLNAGTDAPAQKGIQVTERALKRIRVAMVKEGISPAEG
                     GVRLGVQGGGCSGLSYNVRFDTQPRERDRVFQFEDVRVFIDPKSFIYLNGMTLDYEET
                     LIRQGFNFVNPNSTRSCGCGSSFSA"
     gene            complement(4955..5371)
                     /gene="iscU"
                     /locus_tag="VFA02_12190"
     CDS             complement(4955..5371)
                     /gene="iscU"
                     /locus_tag="VFA02_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HZU10653.1"
                     /translation="MAYSDKVIDHYNNPRNVGQLDKSSQDVGTGLVGAPECGDVMRLQ
                     IKVNPETQVIEEAKFKTFGCGSAIASSSLATEWVKGKTVSEALEIKNTDIVKELALPP
                     VKIHCSVLAEDAIRAAISDWKKKNGVAEAEKQAVAQ"
     gene            complement(5512..6768)
                     /locus_tag="VFA02_12195"
     CDS             complement(5512..6768)
                     /locus_tag="VFA02_12195"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HZU10654.1"
                     /translation="MSSAVENNIPVPAGVKLPIYLDNHATTPLDPRVLEAMMPYFTGK
                     FGNAASRNHSFGWEAEAAVEEARAQVAKTIGATAKEIIFTSGATESNNLAIKGIGEMY
                     RERGNHIITQVTEHKAVLDTCKRMEKSGFRVTYLPVKADGLIDLDDLKRAIDDKTILV
                     SIMFANNEIGVIQPVAEIGKICREKGVIFHTDAVQAVGKIPVDVNAMHIDVLSLSGHK
                     IYGPKGVGALYVRRRNPRVQISEQINGGGHERGMRSGTLNVPGIVGLGKACEIALAEM
                     QEESERLRRLRDKLKAKLEAKLDYIHVNGSMEHRLPGNLNMSFVYVEGESLLMGINDV
                     AVSSGSACTSATLEPSYVLKALGLGDDVAHSSIRFGLGRFNTEAEVDYVADKVIDVVQ
                     KLRELSPLYEMVKEGIDLTKIEWQAH"
     gene            complement(6788..7267)
                     /locus_tag="VFA02_12200"
     CDS             complement(6788..7267)
                     /locus_tag="VFA02_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU10655.1"
                     /translation="MLRLTKKADYALMALKYLAEHPESSALSAKDIAEAYHIPQQLLA
                     KILQRLAKVGMVKSHAGMNGGYVLAKEPDRISAFEVIFAIDGPLFITSCFTQEGNCDL
                     TNSCTIKEPLARVNETISSVLKNIHISDLVEEGRSPMERKLVTIRSASGEKILHHVV"
     gene            7553..7705
                     /locus_tag="VFA02_12205"
     CDS             7553..7705
                     /locus_tag="VFA02_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10656.1"
                     /translation="MTTICKHPRVRIVAREEDAEYVECLDCGEVFDSAEFQDMAIEEE
                     VAEEEE"
     gene            complement(7727..8311)
                     /locus_tag="VFA02_12210"
     CDS             complement(7727..8311)
                     /locus_tag="VFA02_12210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZU10657.1"
                     /translation="MNAFRATPNQLTFLRLCIIPFLVLAILDGYNRTAFALFLIAGLT
                     DGLDGLLARVLHQRTVLGQYLDPVADKLLLSTLFLVLHHEGAISRKVTVLVFARDLGI
                     LVVAAILYAGMGMRNFRPSLFGKANTLAQILALVSVLLSRFYASEWVLFARHWSLQAT
                     IVLTVVSGFHYAWRVARRLGAPSQSAGLKAQSPN"
     gene            8481..9404
                     /locus_tag="VFA02_12215"
     CDS             8481..9404
                     /locus_tag="VFA02_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU10658.1"
                     /translation="MKHTLGFLLLACPIVSAQTAPARPPLTGISHIAVYASDPGAAEH
                     FYTDIVGCQKGTDPEDPQGARYYVNSTQFVEVLPLKDKSSPNRLDHLAYKTTSAEELR
                     LYLKAKGVEVPERVEKAGDGSRWFNVKDPEGNTVQFVQPPARPRAVSAPDLAGHHIIH
                     VGMLVRSREKEDTFYRDILGFRPYWYGGMKPGKIDWVSQQTPESHDWLEYMLTSGPSG
                     GGIQDISQHQLGVLNHLSIGVVNMTKTYEMLKAQNRLAPPDTKTQIGRDGKWQLNVYD
                     PDGTRLEYMEFSNVQPPCCSQFTAPNPSPSE"
     gene            complement(9531..10079)
                     /locus_tag="VFA02_12220"
     CDS             complement(9531..10079)
                     /locus_tag="VFA02_12220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU10659.1"
                     /translation="MSKISLLVIAAVAVIAVVFVSRSFADDNQPMPQVGQLAPNFTLP
                     SQEGKPVSLDSFRGKWVVLYFYPKDMTSGCTIEAHNFQRDQEKFQKANAVILGVSVDS
                     VDSHKQFCAKDGLNFTLLSDADKKVVAQYGSLGNYAGFKIANRNTFLIDPEGKIVKVW
                     TKVDPSHHSEEVLSALNQLEAK"
     gene            complement(10111..10968)
                     /gene="lpxI"
                     /locus_tag="VFA02_12225"
     CDS             complement(10111..10968)
                     /gene="lpxI"
                     /locus_tag="VFA02_12225"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897704.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HZU10660.1"
                     /translation="MKLGLIAGNGRFPFLLLDAARAHGLEVVVAAIKEETDPEMNARA
                     TADSGVRVYWLSLGELSKLIETFHQEGVTRAVMAGQVKHKQIFSSIRPDWRLAKLLLS
                     LRTKNTDMLLGAVAKVLSDEGIELISSTSYLEPLLATPGVMTERAPSEHEQKDIAYGR
                     SIAHTVAGFDIGQTVVVASQACVAVEAMEGTDAAIERAGAIMKTMEAEGDASTFERSL
                     TVVKVAKPKQDMRFDVPVIGVKTIETMEHAGATCLAIEAGRTLVFDKDTIISRANAAK
                     IAIIAVEQG"
     gene            complement(10994..11449)
                     /locus_tag="VFA02_12230"
     CDS             complement(10994..11449)
                     /locus_tag="VFA02_12230"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZU10661.1"
                     /translation="MDLWRISSFHSLSGEGGLRYSARWHTAGRRIVYLAESPAGAMVE
                     VLVHLELNERSWPVHYDLMRIAVPENVEIETFKVRSRPDWRQSFEITRKIGDEWLQSQ
                     RTALARVPSAILPDTWNVLLNPEHPAARRIRIAETIRAKYDDRLFPKVR"
     gene            complement(11449..11862)
                     /locus_tag="VFA02_12235"
     CDS             complement(11449..11862)
                     /locus_tag="VFA02_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HZU10662.1"
                     /translation="MGQLHTTGRHIPFMASLHLDLDAVESGVPVSALKNFVDSSGMQF
                     KDLYEVVIPARTLKHRKAQKERLSTDESDKFARLIRVFDQAERVLGSRAKALHWFHEP
                     KRRFEGRTPIQMLRTELGGRMVEEMLGQIEYGMFA"
     gene            complement(11948..13816)
                     /locus_tag="VFA02_12240"
     CDS             complement(11948..13816)
                     /locus_tag="VFA02_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU10663.1"
                     /translation="MPLDRRAFLALSGQFGLASTLFPGALYTLAAQAQSGPDAEKFPA
                     ITPEMIDQAAALAGLTIAPEQKKMMLDGLNDQRKSYAAIRKLHLPDSVPPAYVFDPLP
                     PGAKVDTEKHPPRYSKAPAVATVPSNLEDVAFASVIELGDLVRRKKVSSVNLTEMYIA
                     RLRRYDPLLHFVITITEDRALAQAREADADIAAGRYRGPLHGLPWGAKDLLAVKGYPT
                     TWGAGGFEKQTFDEDATVVRRLDAAGAVLIAKTTLGALAMGDKWFGGRTRNPWNPKQG
                     SSGSSAGSASATAAGCVAFSVGSETLGSISSPSTRCGTTGLRPTFGFVPRTGAMTLSW
                     TMDKLGPICRAVEDCAIVLEAIYGPDGEDLAARDAAFNWNAEFDWKSLRAGYLKEDFG
                     PVKKPEPLDEKSLDGLSEEEKKKRKQYQNNMNAWRERRAYDLKYDLAALDKLRSMGVD
                     LVPLDLPDLPYDAMTPLLEAEGAAAFDELTRTGRDKLLTEQGPEDWPNLFRIARFYPA
                     VEYIQANRARTLAIREVSKLFEQVDVIVASSGGKQLTVTNLTGHPAVIVPNGIRGNDA
                     PAPPGVDTGDDDSIGGPGTPVSITFLGEHYQDARLCAFAKAYQDATGFHKLHPKLT"
     gene            complement(13870..15741)
                     /gene="aspS"
                     /locus_tag="VFA02_12245"
     CDS             complement(13870..15741)
                     /gene="aspS"
                     /locus_tag="VFA02_12245"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZU10664.1"
                     /translation="MLDFLGTLERTHTCGELRAEDAGKSVVLLGWVNRRRDHGNLIFL
                     DLRDRYGITQVVLDKELSPAAHAKAEHARPEYVIAAIGKVRLRGKDVINPKMATGEIE
                     VVAEELRILNDAKVPPFSPAEDAISNEEVRLKYRYLDLRRAEMQRNFEVRHKVTLAVR
                     DYLSQQNFFEIETPFMTRSTPEGARDYLVPSRVHPGTFYALPQSPQLFKQILMISGFD
                     RYFQIARCFRDEDLRADRQPEFTQIDLEMTFPQQETIFRVVEGFLKAAFAAVGIALPV
                     PFPQITYDEAMRQFGIDKPDLRLPGLTDVKEIFSESDLATLAIDPALPVVALRIPKVG
                     ELSRKERDDNRPLFDTRKGAKYIDDLKRLEKSFPNAVIKLRELTKAEEGDLLIIVAGD
                     AAMHIQASDTKFEGRLSEREIAVYSAAGNFRVELAKKYADRHGAFRVTEEVVASPERK
                     TGLVDGSAAFRPIWVTDFPMFEHDIETGKWMPAHHPFTSPHEEDMDRLTSDPASVRAR
                     YYDLAMNGLELGSGSIRIHRQDVQRQIFSALGMSEEEAKERFGFFLEALQYGTPPHGG
                     IALGLDRLVMILAGANSLREVIAFPKTAKAIDLMVDAPTPVSDTQLKELHIRTALKS"
     gene            complement(15801..17084)
                     /gene="hisS"
                     /locus_tag="VFA02_12250"
     CDS             complement(15801..17084)
                     /gene="hisS"
                     /locus_tag="VFA02_12250"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZU10665.1"
                     /translation="MSTLKAVRGTRDLLPPETELWNFVEETARRVFARYNFGEIRTPV
                     FEDTSLFARGVGEETDIVSKEMYTWEDRARAQSEKSQSLTLRPENTAGVVRAYIEHKL
                     GETGLLQKLYYIGPQFRRERPQKGRYRQFYQIGAEVIGPPSAGSESPLRDAEVLEMLA
                     TLLDELGIQDWKLVINSVGSSSDRPRYVAALREALKDKAPLMCADCQRRAETNPLRVL
                     DCKVPEDQPIIETLPKIADYLDEDSKAHFAAVCAALDACGVQYEINPRLVRGLDYYTR
                     TTFEFTHGGLGAQNALLGGGRYDGLSEALGGPKAPGIGFAMGEDRLILTLQAQAAQQG
                     VLADAYVAPVGEAQNAAALELARELRRAGLRIELGDGGFRLKKSFEIGNKLARKIVLL
                     GEDEVQSGILTVKDFASGEQVKVPREELAAALGAK"
     gene            complement(17111..17401)
                     /locus_tag="VFA02_12255"
     CDS             complement(17111..17401)
                     /locus_tag="VFA02_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10666.1"
                     /translation="MSRATYPLKLPISIKKAAQRLAKEDGVSLNQWIAVAVAEKVGVV
                     ETAAEFFAKRAKKGDKREFLKFLRSVPNVPPLPGDELPPEMVHRRTNRAHSR"
     gene            complement(17398..17808)
                     /locus_tag="VFA02_12260"
     CDS             complement(17398..17808)
                     /locus_tag="VFA02_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HZU10667.1"
                     /translation="MRAVLDTSVIVAGLRSSNGASYALFHEIYSGNITMLVSPPLFLE
                     YEEVLLRKEHNFSKGYVEESLLWIATFAKPVEIYFTWRPQLPDPSDEIVLETAINGMA
                     DAVVTHNMRDFAAAQKDFRLKILRPAELLRMVRV"
     gene            complement(17851..18375)
                     /locus_tag="VFA02_12265"
     CDS             complement(17851..18375)
                     /locus_tag="VFA02_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZU10668.1"
                     /translation="MIRSYQGHTPVIPENCYVDISAQVIGDVVLGHHSSVWMNAVVRG
                     DVNSIRIGAHSNVQDCAVLHGMRNLYKVIVGDYVTIGHNATVHGCTVEDACLIGMGAV
                     ILNNARIGEGSIIAAGAVIPENMVIPPRSLVAGVPGKVRRELTSDDRDLILKYARNYL
                     DYTKIYLEEMKNWQ"
     gene            complement(18451..19275)
                     /locus_tag="VFA02_12270"
     CDS             complement(18451..19275)
                     /locus_tag="VFA02_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZU10669.1"
                     /translation="MLKAISTHVFLRSRLHPGHLEIFARAGAQGVELFAARQHFDYTN
                     RAHVRELAEWFRSNPVEAFSMHAPLFSDLEMGRGGAPAVNVIHPEKSRRVEAMDEIKR
                     ALEVAEQLPFRYLILHLGEREDTWSPRSLENSLTALEHLRAFANPLGVKLLVENIQNE
                     VTQPRNLVEILNVGHFSDIGVCLDTGHAHLGDGVATVLGELRPHIRSSHLHDNKGNRD
                     EHLWPGDGTIQWEETIRELKSAPQTPAGVLEIHYTLEETPESVASKAQAAFARFES"
     gene            complement(19311..20339)
                     /locus_tag="VFA02_12275"
     CDS             complement(19311..20339)
                     /locus_tag="VFA02_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU10670.1"
                     /translation="MDRKPTMNDVARVAGVGTMTVSRVLNGSANVTEETAKRVYRAIE
                     KLGYRPNEMARALRSLKSRTIGLIVPYLYDQFFATCAHAVNTVARDQGYSVMLTTSNN
                     DPAAEYDEAQSMVQRHVEGMVIIPADIRKSRINQSEFGGVHIVTMDRPVHDDRIDSVQ
                     VQNQSGAKRAVQHLIQQHGHKRITFLGYNKNLYTVRARFEGYRRAMLEAGLEPEPTFD
                     CSSEEATAEIIIGMLQNREPATAFFTANNLTTRYALHALIHHGVKVPEEVALAGFDDF
                     ELADILHPTLTVVRQPASELGRVAANLLFDRIKRDEFPEEGSHVVLPVELVIRRSCGC
                     KGRQGRTV"
     gene            20428..>21304
                     /locus_tag="VFA02_12280"
     CDS             20428..>21304
                     /locus_tag="VFA02_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10671.1"
                     /translation="MRRLCFIIAFLLCLPAYAQDTLYAKGRAAFDTSQWNEAASLLAQ
                     AEAASPGTTDALLYEAKALLYLGRLAEAEKTLNSYLTRHPGSADALYALGLVQQRENH
                     PRDSLATMTHAAQLRTPASEDLRIVGLDYVLLNDYPDAIHWLEKAVEFDSKNTEAWYA
                     LGRADYTQSRFHDAEHAFQQALLLDPEHIRATENLGLVYSAENRLPDADETFRKAVML
                     AEKDGHTDEWPYLNYGSFLLDQGRASEAIPLLERATSINPKCAACFEKLGRALSATGK
                     LQEGVEHLEKAVSLDP"
BASE COUNT         4555 a         6456 c         5565 g         4728 t
ORIGIN      
        1 ttttgcggca gtgcatcgag gatgcggcac actcccttga gcacctgaat atcttccttg
       61 tcttcgattt caatctgctc aatcagattc aactcttcaa cgtcattcag gtcggggcgc
      121 aacagccgca ccgtttcgat cgcgcggcgt ccgtgggctg ttctgatggc gattgccggg
      181 tctactccgc gctttgcggc ccacttctgc cagctccgct cgactgagcc gagagaactg
      241 accagaacac catccatatc aaacagaatt ccgcgaacgt gcagcttcac ttgtcgtgac
      301 tcctaaatat tgggcgtgcc gaaaagactg agtggcgagg atacgaggac tccatctcgt
      361 aaataacgcg ggtagaagca gcggctttgg ccctttgaat caagaggctt cggccccggc
      421 cgggtctact ccgcgccttc tttgaattcc tcgtaccatg ccatctgaat ggcttcgagt
      481 ttcggttcat ttgactttgc cggatcatcg gcaaaacctt caagctccgt cacgtatttg
      541 tgcagatcgg taaagcggac ggtgagcggg tcaacgtcag gaaacttttc gtagagctgg
      601 atgccgatct cttccgcgtc tgtccagtag atttcacgcg ccattatttc ttctcctctg
      661 ccagggccag cggtttgcct tcagaaacat agttgcggtt ccacttggga atctcgacga
      721 cataattgcc cggcctggta atcacgcatt ggcagccaag acgcgagttg agttgcagat
      781 cggcggccat gtccagacgg tccagctcat catcgtccgc ttcgctcagt ccctcggcgc
      841 cctctttgat ccatacatgg cacgtggtgc aggcgcatgc cccaccgcag gcgtggtcaa
      901 gaaagatgcc gtagttttcc gccacgtcga gaaacgacat cggcttgccg tgatggtcat
      961 agggcatggt gccaaattca aactcgaccg tcttgccctc aggctgaaat gtcacacgca
     1021 ccatgtttgg cgcgggtggc gtgctcagat ctatgttgtg gtttgttttt ccttcgctca
     1081 tagttgccag ttgttagttc ctgggtgccc cacatcctga agggatgcgg agttatttca
     1141 tcccttcgta atctccgcag gcgcaaatgg atgcggcgtt gtcggccctt cgcccaattt
     1201 attgcctgcc gaatccatcg tttcgccctt aatggcgctc gatacagcag catccatcat
     1261 cagctccgca aagcgccgcg tggccttgtc cagggcgtcc gttccagccc gcagtgcctt
     1321 caggtcgtct tcttgtttca ggacggaaag ctcttctttc agcagcgcaa tctgtgcgcg
     1381 ctcttcatct gtcaactgct gccatgccgg attctccggg gccttctcta ccgcgcgcag
     1441 aatggtatcg gcctcgttgc gcgcttcaat cagttgtcgc tgatagaagt cctcttccgc
     1501 atggtcgaac gagtcgagaa tcatcgtctc aacctgctcg tctgtaaggc catacgccgg
     1561 ctttacttcg atttcggcct ctttgcccga gcgctgctcg cgcgccgaga cgtgcagaat
     1621 accgttcgca tcaatcagga atttcacctc gatgcgcggc agtccggcag acatcggcgg
     1681 gatgcccttc aggtcaaatc gtgccagcga gcggcagtct tttgccaact cacgctcgcc
     1741 ttgcaccacg tggatggcca cattcgtctg tccgtctacg cctgtggtga aatgctcagt
     1801 ggccgatgca ggaatggtcg aattgcgctg gatgatcttc gccaccactc cgcccagcgc
     1861 ttcaatgccc agcgaaagcg gggtcacatc cagcagcagc atgtcttccg ttgccttgga
     1921 tccgccagca agaatgttcg cctgcaccgc cgcgcccagc gcgacgactt catccggatt
     1981 cagctccgta tgtggtttct tgccgcgcgc gcttagatga aagacctcat ccgtcagctg
     2041 gcggaccgca ggaatgcgcg tcgagccgcc aaccagaacg acttcatcaa cctgctccgg
     2101 agtaacacct gcatctttca gcgcctgact gcagggtgtg atggtcttcg caagaatcgg
     2161 gcggataatc tcctcaaagg ctgcgcgagg aagcatgcgc tgatacttgt gcccgccagg
     2221 cagatccacg ctgatgcgcg cggcctcaga agaagacagc tcaatcttgg cttcaatcac
     2281 ggccttgcga atggcctgca cagcttcggg gctggaactc aggtcaaggc caagctcgcc
     2341 tttgatgtct tcaagcgcaa tcgcaatcag cagattgtca atatcatcgc cgcccaggtg
     2401 cgtatcgccg ttcgtcgcaa tgacctcgaa gatgccctcg tgcagtttaa gaatagagat
     2461 gtcgaaggtc ccgccgccca gatcgtagac ggcgacaatg ccttccttat tgcggtccag
     2521 gccataggcc agcgctgctg ccgttggctc gtttaccagg cgcagcactt cgagtccggc
     2581 catgcggccc gcgtcttttg tcgcctgacg ctgcgcgtca ttaaagtatg caggaacggt
     2641 gatgacggcc ttcgtcaccg gagcgccaaa ataccgctcg gcattgcgct ttaactggcg
     2701 caaaacatag gctgaaatct ccggtggagt aaactcttcc tcgcccagcc ggatacgcag
     2761 cacttctccc ggttgcaaac cggcagcaag acgaaacgga aagagcctca gctcttcctg
     2821 cacatcttca atgccacgcc ccattagccg ttttgccgaa tataccaccc gctccggcgt
     2881 gcggaccaga tgcgcgcgcg ccgcattgcc gacgatgaat ctgcgctggc cattggcaga
     2941 atcaagcgca actactgagg gcacaaggtt cgatccgtcc tcgccaggga tgaccacagg
     3001 cttctcgccc tgcataaaag caacaaggga attggtcgtg cccagatcaa ttccaacaat
     3061 gcgttccttg ttttcgctca caccaatacc tcaggtttct gttgcatggg aggattcaaa
     3121 acatactgtg cctcaccctt gccgaaggac cagtccgcca gatcgttctc tgaaagcaca
     3181 atcatcacat cgtccggagc gatgccggcg cgctcctgcg ccagacgcac aatttcgctg
     3241 tacagcttct gcttcatctc aatggtgcgt ccgcgccgca aagtaatgtg gacgacggtg
     3301 aagccgtccg tgcgctgcat tcccatgaat gtcgggtctg ctatcagttc gccctcaccg
     3361 tgcgcatgca acgcgacaaa caaatcgttc tccggaatgt tgatggaagc gcgcatcgca
     3421 tcgtaaacag ctttgggaag ctgccggcgc ttctccaccg gcatcttctc tgaaacagaa
     3481 atgcggacaa acggcatgaa atcctctttc ttctatgtca tcctgaggcc ggggctgccc
     3541 cccatctcgc agagatggga aggccgcaac atgcgcccat cacccaaata ctcacttcag
     3601 ttccagcgct tcattcacat cacgcaccag gttgcgcaca taccggcgcc ggtcgagcag
     3661 cgccaccata cggtccttcg catcatccag cgacgcgttg tcccgcgcat cgagcgctgc
     3721 atcccacttc tcccagagtg attgcagctg cgtgtctgca tcagcaagct gcccctcaaa
     3781 ctgcgccttg gctttttcga gatcgcccct cagttgcgca tcgtcctcgc ccatcttctg
     3841 gttcatgcgc atctcttcaa gctgcatgtt cagctcgaag acttcttcca gaagatcagc
     3901 cggtacccgc ggttctttct ccccattcgc cgcggccttg tcctgctcca tctctacgcc
     3961 ttcaagccgc agcagatatt ccgtgcgcga gatgggattt ttcagcgtgc gataagcatc
     4021 attcagcagc gaggtctgct ccaggctcca ctgctgctcc ttcgcgcagg cctgggcgta
     4081 aacgtcagga tgcagtttcc ggctcagctt atagaaggcg cgctccagcg cagatagatc
     4141 aagcgccagc ttccgcggca atccaaagac atcaaagtac gtcatgctgc tcaccggggc
     4201 ctgtaccttt ccacaggtat cgcagaacgg ccgccccgag gacatctcag aaccgcacga
     4261 ccagcacgcc gtcttcacgg attgttgttc gatagtttgc attgaaggac tctagctgaa
     4321 tcgcccgcaa ttttgcgccg taaggcgcga tcgcctggac ggcaagtcct aggctgaaaa
     4381 cgacgaccca cagccgcagg accgcgttga atttggattt acgaagttga agccctggcg
     4441 tatcagcgtc tcctcataat ccagggtcat cccgttgaga taaatgaaag acttcgggtc
     4501 tataaagaca cgcacatctt caaactgaaa cacacggtcg cgctcgcgcg gctgcgtatc
     4561 aaagcgcacg ttatacgaaa gccccgagca cccgccgccc tggacgccca gacgcacgcc
     4621 gccttctgca ggcgaaatcc cttccttcac catcgccacg cggatgcgct tcagcgcgcg
     4681 ctcagttacc tgaatgcctt tctgcgcggg agcatcggtc ccggcattca gcacattgag
     4741 ttgtgtgctc atagtaagtc cttcaacatc agctagcgca ttttcacaga tagtctggac
     4801 catgcccgcg aaacttcagt cgccgcgacc aacagggagt cggcaacact gcacagtgca
     4861 aatggagtgt acagaatctg taaaaatgcc gtagtctcca gtcccaaggt tgctggtctc
     4921 cgtttcaggt catctgagga ctggcaacga acaactactg cgcaaccgcc tgcttctccg
     4981 cctcagcaac gccgtttttc ttcttccagt cgctgatggc cgcgcgaata gcatcttccg
     5041 ccagcaccga gcagtggatc ttcaccggag gcagcgccag ctccttcacg atatcggtgt
     5101 ttttgatctc gagtgcttca gaaacggtct tgcccttcac ccattccgtc gccagtgaag
     5161 atgaagcaat tgccgacccg cagccgaatg tcttgaattt ggcttcttca atcacctgcg
     5221 tctctggatt gactttaatc tgcaaacgca tcacgtcacc gcactccggc gcgcctacaa
     5281 gtcccgtgcc tacatcctgg ctggatttat caagctgccc aacattgcgc ggattgttgt
     5341 agtggtcaat caccttgtcg ctatatgcca ttcttttctc ctcttaatct caaaaaccgg
     5401 ttgtcatcct gaccgaagcg accgaaggaa gcgcagtcga aggacctcca tgctccccgg
     5461 tcacggcctc tcaaccagcc ccagcctgct cacaacacag gggaactact ctcaatgtgc
     5521 ctgccactca atcttggtca gatcaatgcc ttccttcacc atttcgtaca gcggtgaaag
     5581 ctcgcgcagc ttctggacta cgtcaatgac tttgtcggcc acgtagtcca cttcggcttc
     5641 ggtgttaaag cgccccagtc caaagcggat ggagctgtgc gccacatcat cgcccagccc
     5701 cagagccttc aacacatacg atggttcgag tgtggccgag gtgcaggccg aaccactcga
     5761 aaccgcgaca tcgttgatgc ccatcagcag cgactcgcct tctacataaa caaagctcat
     5821 gttcagattg cccggcagac ggtgctccat cgagccgttt acgtggatgt agtcgagctt
     5881 tgcttcgagc tttgccttca gcttgtcgcg cagcctgcgc agacgctccg attcttcctg
     5941 catctctgcc agcgcaattt cgcacgcctt gcccagaccc acaatgcccg gaacattcag
     6001 cgtgcctgaa cgcattccgc gctcgtggcc gccgccattg atctgctcgg atatctgcac
     6061 gcgcgggttc cgccgccgga catacaatgc gcccacgccc tttggcccgt aaatcttgtg
     6121 cccggaaagc gagagaacgt caatgtgcat cgcattcacg tccaccggaa tcttgcccac
     6181 ggcctgcact gcatcggtgt ggaagatcac gcccttctcg cggcagattt tgccaatttc
     6241 tgccaccggc tggatgacgc caatttcatt gttggcgaac atgattgaga ccaggatggt
     6301 cttgtcgtct attgcgcgct tcaggtcatc aagatcaatc agcccatcgg ccttgactgg
     6361 caggtaggtc acgcggaagc cggacttctc catgcgctta caggtatcga gcacggcctt
     6421 gtgctcggtc acctgtgtga tgatgtggtt gccgcgctcg cggtacatct cgccaatgcc
     6481 tttaatggcc agattgttcg attcggttgc acccgaggta aagataatct cttttgccgt
     6541 cgcgccaatg gttttggcaa cctgcgcccg cgcctcttcc accgccgcct cggcctccca
     6601 tccaaaggag tggttgcggc tagcggcatt gccaaacttg cccgtgaaat agggcatcat
     6661 tgcctccagc acgcgcggat cgagcggcgt ggttgcatgg ttgtccaggt aaatcggcag
     6721 cttcacgcct gccgggacgg ggatattatt ctccactgcg ctgctcatgg ggcttttctt
     6781 ctctcctcta aactacgtgg tgcaaaatct tttcgccgct tgctgaacgg atggtcacta
     6841 acttgcgttc catcgggctg cggccttctt ctaccaggtc ggaaatatgg atgttcttca
     6901 gcacgcttga aatcgtctcg ttcacgcgcg ccagtggttc tttgatggtg caactgttgg
     6961 tcaggtcgca gtttccttcc tgcgtaaagc acgaggtgat aaacagcggc ccatcaatcg
     7021 caaaaatcac ttcaaaggca gaaatccggt caggctcctt tgccagcaca tacccgccat
     7081 tcatgccggc atgggacttg accatcccca cctttgccag ccgctgcaaa atctttgcca
     7141 gcagttgctg cggaatgtgg tatgcctcgg cgatgtcctt ggcgctcagc gcgctggact
     7201 caggatgctc cgccagatac ttcagcgcca tgagcgcata atccgccttt ttggtgagcc
     7261 ttagcattgg aaattaccgc aaaaatgggg tactttttgc ccagttgtct attgcaagta
     7321 tacgaccgct ggagtccgga ttcaactcat tcgcacacaa taataattat cttctaatag
     7381 aagttcataa aatggtcccg aagctggtgt gtttttgctc ctgctctgcg gaacgccgtc
     7441 tgcctgccgt tcgtatctct ttcctgcaag atttaccgga ttcctgtttg acaaaggaaa
     7501 caatacggat aaaattttgc cgatatcacc tgtttacggc ccagaacacc ccatgaccac
     7561 gatttgcaaa caccccagag tgcgcatcgt tgcgcgtgaa gaggatgccg aatacgtaga
     7621 atgcctcgac tgtggcgagg tattcgactc cgccgaattt caggacatgg ccatcgaaga
     7681 agaagtcgcc gaagaagagg agtaggggcg cagtaccata gtccttctaa ttggggctct
     7741 gggccttgag acctgcgctc tgtgaaggcg ccccaagtct ccgtgccact ctccacgcat
     7801 aatggaagcc ggaaaccacc gtcagcacaa tcgtcgcctg caacgaccag tgccgggcaa
     7861 acaaaaccca ttcagaagca taaaaccggc tcagcaggac tgataccagc gccagaatct
     7921 gtgccaacgt gttcgccttt ccaaacaggc tggggcggaa attacgcatc cccatgcctg
     7981 catatagaat cgccgctacc accagaattc ccaggtcccg cgcaaagacc agcaccgtca
     8041 ctttgcgtga gatcgctcct tcatggtgca gcaccagaaa aagcgtgctc agcagcagct
     8101 tgtctgcgac cgggtcaaga tactgcccca ggaccgtccg ctggtgcagc acccgcgcta
     8161 acagcccgtc cagcccgtcc gtcagccccg caatcagaaa cagcgcaaac gctgtgcggt
     8221 tgtagccatc cagaatggcc agcacaagaa agggaatgat gcacagccgc agaaacgtaa
     8281 gctggttcgg agtggctcga aatgcgttca ttcgcgcaga aaatagtgca gtatgaagct
     8341 ttgatgttac aggaaaacta cgccttgggc ccaaaggctt cgcagcaatg ctgttcctcc
     8401 tgggacccgg ctccgcaaca aagtccggcg agggggccgg ctgacaaacc gtctctgcgc
     8461 gcggtactct tgaccgtgca atgaaacaca cgcttgggtt tcttcttctc gcctgcccta
     8521 ttgtttcagc tcagaccgct ccagcgcgtc cgccgcttac cggaatctcc cacatcgccg
     8581 tgtatgccag cgaccctgga gcagcagagc atttctacac ggacattgtc ggttgtcaga
     8641 aggggactga tccggaagac ccgcagggcg cccgttatta tgtcaacagc acgcaatttg
     8701 ttgaggtgct tccgcttaaa gacaaaagct cccccaatcg tctcgaccat ctggcatata
     8761 agaccacgag tgccgaagaa ctccggctct atctcaaggc aaagggtgtg gaggtccctg
     8821 agagagtcga aaaagccggc gatggaagcc gatggttcaa tgtgaaggac cccgaaggaa
     8881 ataccgtcca gttcgtccag ccgccagcgc gtccgcgcgc agtcagtgcg ccagatctgg
     8941 ccggacacca catcatccac gtcggtatgc ttgtccgcag ccgcgaaaaa gaggacacct
     9001 tctaccgcga catcctcggc ttccgtccct actggtacgg aggcatgaag ccgggaaaaa
     9061 ttgactgggt ctcgcagcag acaccggaaa gccacgactg gctcgaatac atgcttacca
     9121 gcggcccgtc cggcggaggc attcaggaca tctcgcaaca tcaactcgga gtgctgaacc
     9181 acctttccat tggtgtggtc aacatgacga agacttatga gatgctgaag gcccagaacc
     9241 gcctcgcgcc gccggacacg aagacccaga ttggacgcga cggcaaatgg cagctcaacg
     9301 tctacgaccc cgatggaacg cgcctggaat acatggagtt ctccaatgtg cagcctccat
     9361 gttgctcgca gtttaccgcg cctaatccgt cacccagcga atgatgcctt tgccgccgaa
     9421 ccaagcatgt gtacctgttt gcgtgtccat ccgccttcgc gccacggtac agaggccaga
     9481 gtcaggcgag gcgtataccg cctcgcacct tgcaagaaac tcctgcgcgg ttacttggct
     9541 tccaattgat tgagtgccga aagcacctct tcgctgtggt gtgacgggtc caccttcgtc
     9601 cacaccttca cgatttttcc ttcaggatca atcaggaagg tattgcggtt ggcaatcttg
     9661 aagccggcat aattgccgag cgaaccatac tgcgccacca cttttttgtc tgcatcgctc
     9721 agaagggtaa agttcaggcc gtcctttgcg cagaactgct tgtggctgtc cacagaatcc
     9781 acgcttacac ccagaatgac ggcattggct ttctggaact tctcctggtc gcgctggaag
     9841 ttgtgcgctt cgatcgtgca gcccgaggtc atgtctttgg gatagaagta gagcacgacc
     9901 catttgccgc ggaagctatc gaggctgacg ggcttgcctt cctgtgaagg aagagtgaag
     9961 ttcggggcca gttggcccac ctgaggcatg ggctggttat catcggcaaa gctgcgcgat
    10021 acaaatacaa ccgcaataac ggcgacggct gcaatcacaa gcagagaaat cttagacacg
    10081 acgcaaagtc ctcctgtatg ggtgcgctta ttatccttgt tccacagcaa taatcgcaat
    10141 cttcgcggca tttgcccgcg aaatgattgt gtctttatca aaaaccagtg tgcgtcctgc
    10201 ttcaatcgct aggcaggtag cgcccgcgtg ctccatcgtt tcgatggtct ttacgccaat
    10261 caccggaaca tcaaaacgca tgtcctgttt cggcttggcg accttcacga cagtaagact
    10321 gcgttcgaat gttgaagcgt cgccttcggc ctccatcgtc ttcataatgg ctccggcacg
    10381 ctcgatggcc gcatccgtcc cttccatcgc ttcgaccgcg acacaggcct ggctggcaac
    10441 cacaacagtc tgtccaatgt cgaatccggc aacggtatgc gcaatggagc gcccataagc
    10501 gatgtctttc tgctcatgtt ctgacggcgc ccgctcagtc atcactccag gcgttgccaa
    10561 aagcggctcc agatacgaag tagacgagat cagctcgatg ccttcatcgc tgagcacttt
    10621 ggcgacagcg cccagcagca tgtcggtgtt cttcgtgcgc agagaaagca gcagctttgc
    10681 cagccgccag tccggtcgga tgcttgaaaa aatctgcttg tgcttcacct gtccagccat
    10741 cacggcgcgg gtgacgcctt cctgatggaa ggtctcgatg agcttcgaca actctccgag
    10801 agagagccag taaacacgca ccccggaatc agccgttgcg cgcgcattca tctccggatc
    10861 ggtctcttcc ttaatggcgg cgacgacaac ttcaagtcca tgcgcgcgtg ccgcatccag
    10921 cagcagaaaa gggaagcgtc cattgcctgc aatcaatccg agcttcatgg gtaatagcag
    10981 agtagcatct gcgttagcgc accttcggaa acagccggtc atcgtacttg gccctgatgg
    11041 tttctgcgat tcggattcgc cgtgccgccg ggtgctccgg attcagcagg acattccagg
    11101 tgtccggcaa aatggcggaa ggcacacgcg ccagagcagt ccgttgtgat tgcagccact
    11161 catctcctat tttgcgggtg atctcgaatg attgtctcca gtctggccgt gacctgactt
    11221 tgaatgtttc tatttccacg ttttccggca ctgcgatgcg catcagatcg tagtgcacag
    11281 gccaacttct ctcatttaat tccagatgga ccaggacctc caccatggct cctgccggag
    11341 attcggcgag atacacaatc cgtctccccg ccgtatgcca gcgggcagaa tagcgcaggc
    11401 caccttcgcc tgataacgaa tgaaagctgc tgatacgcca aagatccatc aggcaaacat
    11461 gccgtattcg atctgaccaa gcatttcttc aaccatgcgg ccgcccagtt cggttcgcag
    11521 catttgtatc ggagtacggc cttcgaaccg ccgcttgggt tcatggaacc agtgcagcgc
    11581 ctttgctctg ctgcccagca cccgctcagc ctggtcaaag acgcgaatca aacgggcgaa
    11641 cttgtcggat tcgtctgtgc taaggcgctc tttttgtgct ttgcggtgct tgagtgtgcg
    11701 cgccggaatc accacctcgt agaggtcctt gaattgcatc cccgaggaat caacaaaatt
    11761 tttgagggcg cttacaggta ctccggattc aactgcatcc agatcaagat gaagcgaggc
    11821 catgaatgga atgtgccgcc cggtggtatg cagttgtccc atgaggacat tatgccacaa
    11881 ataagtaggc agattgtcca cacctcttcc caccacatgg gcacaagttg gagaaggaaa
    11941 cggcctgcta agtcagcttc gggtgcagct tatggaaccc ggtcgcgtcc tgatatgctt
    12001 tggcaaaagc gcacagcctg gcgtcctgat aatgctcgcc caggaacgtg atgctcaccg
    12061 gagtgccagg gccgccaatg gagtcgtcat cacccgtatc cactcccggc ggagcaggtg
    12121 catcatttcc gcgtatccca ttggggacaa tcacggcagg atgcccggta agattggtga
    12181 ctgtaagctg tttacctccg gaagaagcca caatcacatc cacctgctca aataattttg
    12241 agacttcgcg aatcgccagc gtgcgcgcgc ggttcgcctg aatgtattcg accgcaggat
    12301 agaaccgggc aatacgaaag agattgggcc agtcttccgg gccttgctct gtcaacagct
    12361 tgtcacgccc tgtccgtgtg agctcgtcga atgcggcagc gccttctgct tccagcagcg
    12421 gagtcattgc gtcataaggc aagtcgggca gatcgagcgg gacaagatcc actcccatgc
    12481 tgcgcagctt gtccagcgcg gcaagatcgt atttcaggtc gtatgcgcgg cgttcccgcc
    12541 aggcattcat attgttctgg tactgcttgc gttttttctt ttcttcttcg ctcagcccat
    12601 ccagcgattt ctcatccagc ggttcaggct tcttcacagg tccaaaatct tctttgagat
    12661 atccggcccg cagtgatttc caatcgaact ccgcattcca gttgaaggcg gcatcgcgcg
    12721 ctgccaggtc ctctccatca gggccataaa tggcttcaag cacgatggcg cagtcttcta
    12781 ccgcgcggca gataggcccg agtttgtcca ttgtccagct cagggtcatg gctccggtgc
    12841 gcggaacaaa gccgaatgta ggccgcagtc ccgttgttcc gcagcgcgtt gaaggcgatg
    12901 agatagagcc gagcgtttcg gacccgacgg aaaatgctac acatcctgct gccgttgctg
    12961 aagccgatcc tgccgacgag ccactggagc cttgtttcgg gttccacgga ttgcgcgtgc
    13021 gtccgccaaa ccacttgtcg cccatggcga gcgcgcccag cgtggtcttg gcaattaaca
    13081 cggcccctgc ggcatccagg cgcctgacca cggtagcgtc ttcatcaaag gtctgcttct
    13141 cgaatccgcc cgcgccccat gtcgtggggt atccttttac cgctagcagg tccttggcgc
    13201 cccacggaag cccatgcagc ggcccgcgat atcttcccgc cgcaatgtct gcatcagctt
    13261 cacgcgcctg cgccaaagcg cggtcttccg tgatggtgat aacaaagtgc agcagcggat
    13321 catagcgcct gagccgtgca atatacattt ctgtcaggtt gactgaagaa accttcttcc
    13381 ggcgcacaag atcgccaagt tcaatcactg atgcaaaagc cacgtcttcc agattagatg
    13441 ggaccgtggc aactgccggc gctttgctgt aacgtggagg atgcttctcc gtgtccacct
    13501 tcgcgccagg aggcagcgga tcaaaaacat aggccggagg aacgctgtca ggcagatgca
    13561 gcttgcgaat cgcagcatag gacttgcgct ggtcattcag cccatcgagc atcatcttct
    13621 tctgctcagg cgcaatcgtg aggccagcca gcgctgcggc ctggtctatc atttccggtg
    13681 taatggcagg gaacttttct gcatccggtc cgctctgcgc ctgtgctgca agcgtataga
    13741 gcgccccggg aaagagggta gaggcaagcc caaactgtcc ggacaaggca agaaatgcgc
    13801 ggcggtcaag cggcatggag tcctactctc tgaattgaaa atggcagtat accctatgcc
    13861 gttataggtt caggacttca gcgctgtcct gatatgcaac tccttcagtt gcgtatcgct
    13921 gaccggggtc ggcgcatcca ccatcaggtc aatggctttt gccgtcttgg ggaaggcgat
    13981 gacctcgcgc aggctgttgg cccctgccag aatcatgacc aaccggtcca gcccaagcgc
    14041 aatgccgcca tgcggaggcg ttccgtactg taaagcctcc aggaaaaatc cgaagcgttc
    14101 ttttgcctct tcttcagaca tgcccagcgc gctgaagatc tgccgctgca catcctgccg
    14161 gtgaatacga atcgagcctg agccaagctc aaggccattc atcgccaggt cgtaataacg
    14221 tgcgcggacc gaagccggat cggatgtgag ccggtccatg tcctcctcat gcggcgaggt
    14281 gaagggatgg tgcgccggca tccatttccc cgtctcaatg tcgtgctcga acatcgggaa
    14341 gtccgtcacc cagatagggc ggaatgcagc actgccatct acgagaccgg ttttgcgttc
    14401 gggagatgca acaacctctt cggtcacgcg gaaagcaccg tgacgatcgg cgtatttttt
    14461 cgccagttcg acacggaagt ttccggctgc tgaataaacg gcaatctcgc gctcagataa
    14521 acgtccctca aacttcgtat cgctggcctg aatgtgcatg gcagcgtctc cggcaacaat
    14581 gatgagcagg tctccctctt cggccttcgt cagttcgcgc agtttgatga ctgcattcgg
    14641 aaaggacttc tccagacgct tgaggtcgtc aatgtacttc gcgcccttgc gcgtatcaaa
    14701 gagtggacgg ttgtcgtcgc gttccttgcg cgaaagctcg ccgactttgg gaatccgcag
    14761 cgccacaaca ggcagcgccg gatcaatcgc cagcgtggct agatcactct ctgagaatat
    14821 ctcttttacg tcggtcaacc cgggcaggcg tagatcaggc ttatcaatac cgaactggcg
    14881 catggcctcg tcataagtaa tttgcggaaa cggcacaggc agcgcgatgc cgacagcagc
    14941 aaaggcggcc ttcagaaagc cttccaccac acggaagatc gtttcctgct gggggaaggt
    15001 catttcaaga tcaatctgtg tgaactcggg ctggcggtcg gcacgcaggt cctcatcgcg
    15061 gaagcagcgc gcaatctgga agtagcggtc gaagcccgag atcatgagga tctgcttgaa
    15121 caactgtggc gattggggca gcgcgtaaaa cgttcctggg tgcacgcggc tcggcaccag
    15181 gtaatcgcgt gcgccttccg gcgtcgagcg cgtcatgaag ggcgtctcaa tctcaaagaa
    15241 attctgctgt gagagataat cgcgcaccgc cagcgtgacc ttgtgccgca cctcaaagtt
    15301 gcgctgcatc tcggcgcggc gcaggtcgag atagcggtac ttcaggcgca cttcttcatt
    15361 gctgatggcg tcttcggcag gcgagaacgg aggcactttc gcatcattga gtatgcgcaa
    15421 ttcttcggca acaacctcaa tctctcccgt cgccattttg ggattgataa cgtccttgcc
    15481 gcgcagccgc accttgccaa tcgcggcgat aacatactcc ggccgcgcgt gctcggcctt
    15541 cgcgtgggca gcggggctta gctccttgtc gagcaccacc tgcgtaatgc cataccggtc
    15601 gcgcagatca agaaagatga gattgccgtg gtcgcggcga cggttgaccc atccgagaag
    15661 gacaacagat tttccggcgt cttcagcgcg aagctcgcca caagtatgcg tgcgttccag
    15721 tgttcctaaa aaatccagca aagtttcttc cttagaaaat caaacggatg tcatcctgag
    15781 agaagcgaag ggcctgcttt ttattttgct ccgagcgcag cagctagttc ctcccgcgga
    15841 accttcacct gctctccgct cgcgaagtcc tttaccgtca ggataccgga ttgcacctca
    15901 tcttcaccca gcagaacaat cttccgcgct aatttgttcc caatctcgaa cgactttttc
    15961 agacggaacc cgccatcgcc cagctcaatg cgcaatccgg cgcggcgcaa ctcacgcgcc
    16021 agctccagcg cagcggcatt ctgtgcctca cctaccggag cgacatacgc atcagccagg
    16081 acgccctgct gggcggcctg cgcctgaagc gtgagaatca ggcggtcttc gcccatggca
    16141 aagccgatac ccggcgcctt tggtccgccc agcgcttcag acagtccgtc atagcgcccg
    16201 ccacccagca gtgcgttctg cgcgcccagt cccccatgcg tgaactcaaa agtagtgcgc
    16261 gtgtagtagt ccagcccgcg caccaatctc ggattgattt cgtactgcac tccacacgca
    16321 tcgagcgccg cgcagaccgc cgcaaagtgc gccttcgagt cctcgtccag ataatcggca
    16381 atcttcggca gcgtttcgat gatcggctgg tcttccggca ccttacagtc aagcacgcgc
    16441 aaaggattgg tctccgcacg gcgctggcaa tcggcgcaca tcagcggggc cttgtctttg
    16501 agcgcctcgc gcagggccgc cacatacctc ggacggtccg acgatgatcc cacagaattg
    16561 atgaccagct tccagtcctg aatccccagc tcatcgagca gagtggccag catttccagc
    16621 acctcggcat cgcgcagcgg cgattcggac ccggcactcg gcggcccgat gacctccgcg
    16681 ccaatctggt agaactgccg gtagcgcccc ttctgcggac gctcgcgccg gaactgtggg
    16741 ccaatgtaat agagcttctg caacagcccc gtctcgccca gcttgtgctc aatataagcg
    16801 cggaccaccc ccgccgtatt ctccggccgc agcgtcagcg actgcgactt ctccgactgc
    16861 gcccgcgcgc ggtcttccca cgtatacatc tctttggaga cgatgtcggt ctcttcgccc
    16921 acgccgcgcg caaaaaggct ggtgtcctca aaaacaggcg tgcggatctc accgaagttg
    16981 tagcgcgcaa agacgcgccg cgccgtctct tcgacaaaat tccagagttc agtttccggc
    17041 ggcagaaggt cccgcgtgcc gcgaacggct tttaatgtgc tcatttgctt gatttaaatt
    17101 cacttccgga ttatcggctg tgggcccggt tcgtgcggcg gtgcaccatc tcaggcggca
    17161 gttcgtcgcc aggaagcggt ggaacatttg gcacactgcg aagaaattta agaaattccc
    17221 gtttgtcgcc cttctttgca cgcttcgcaa aaaactcagc cgcggtttcc accacgccaa
    17281 ctttctccgc aacagccaca gcaatccact ggttcagaga aacaccatct tcctttgcca
    17341 atctctgcgc ggccttctta attgaaatgg gcagtttcaa gggatatgtt gctctactca
    17401 tactcgtacc atccttagca attcagccgg acgcaatatc tttaagcgaa aatctttctg
    17461 ggccgcagca aaatcccgca tattgtgagt aacaactgca tcggccatgc cgttgatagc
    17521 ggtctcaagt acaatctcgt ccgatggatc aggaagttga ggccgccacg taaaatagat
    17581 ttcgacaggc ttcgcgaacg ttgcaatcca caagagcgat tcctctacat aacccttcga
    17641 aaaattgtgc tcttttctta gcaggacttc ttcgtactcc agaaacaacg gaggacttac
    17701 caacattgta atgttgccgg aatagatctc gtgaaacaac gcatacgacg ctccattgct
    17761 gctccgcaat cctgccacga tcactgaagt atccagaaca gcccgcactt tacgagcata
    17821 gcatgagaca tcctatagga tgtcttccgt tcactgccag ttcttcatct cttccagata
    17881 aatcttcgtg taatccagat aattccgtgc gtatttcaga atcagatcac ggtcatccga
    17941 cgtcagctcg cgccgcactt tccccggaac gcccgccacc agcgacctgg gcggaatcac
    18001 catgttttca gggatcaccg cacccgcggc gataatcgaa ccctcgccaa tgcgcgcatt
    18061 gttcagaatc accgcgccca tcccaatcag gcatgcatct tcaaccgtgc agccgtgaac
    18121 agtggcattg tgtccaatcg tcacataatc gcccacaatc actttgtaaa gattgcgcat
    18181 tccatgcagc acggcgcagt cctgaacatt actgtgcgcc ccaatgcgga tcgagttcac
    18241 gtccccacgc acgacagcat tcatccacac actggagtgg tgcccgagca caacatcgcc
    18301 gatgacctgc gctgaaatgt ccacatagca gttttcagga ataactgggg tatgtccctg
    18361 ataagaacga atcatgacca gaatttcctt tgttcttcca gtgtagagcg aacgccggaa
    18421 tcaggaggta tcgagtgcga gttctgctgc tcagctctcg aatcttgcaa atgcagcctg
    18481 tgccttcgat gccacgcttt caggtgtttc ttcgagcgtg taatggatct cgagcactcc
    18541 cgccggggtc tgcggcgcag acttcagctc ccggatggtc tcctcccact ggatggtgcc
    18601 gtccccgggc cagagatgtt catcccggtt gcctttgttg tcgtgcaggt gcgaggagcg
    18661 aatgtgcgga cgtaattctc ccagcacggt cgcaacaccg tctcccaggt gggcgtggcc
    18721 tgtgtccagg cagacgccga tatcgctgaa gtgccccaca ttcagaatct ccacaagatt
    18781 gcgcggctgc gtgacctcat tctgtatgtt ttcgaccagc aacttcacac cgagcggatt
    18841 ggcaaaggcg cgcaggtgtt caagcgcggt cagggagttt tcaagcgacc gtggactcca
    18901 ggtgtcctcg cgctcaccca gatgcaggat gagatagcga aacggcaatt gttcggcaac
    18961 ttcgagggcg cgcttgatct cgtccatcgc ttctacgcga cgcgatttct caggatggat
    19021 cacattcacg gcgggtgctc cgccacggcc catctcaaga tcggaaaaca gaggcgcgtg
    19081 catggaaaat gcctcaaccg gattggaacg gaaccactcc gccagctcac gcacgtgcgc
    19141 acgattggtg tagtcaaagt gttggcgcgc tgcaaacagc tcgactccct gcgcgccggc
    19201 gcgggcaaaa atttcaagat gtcccggatg cagacggctg cgtaaaaata cgtgtgtaga
    19261 gatggccttt aacataagaa cttatctcta tcttctcatg tggaaacggg tcagacggtc
    19321 ctcccctgcc gtcctttgca accgcaggac ctgcgtatca caagttctac gggcaagacc
    19381 acatgactgc cttcttctgg aaattcatcg cgtttgatac ggtcgaagag cagattggcg
    19441 gcaacgcggc ccagttccga tgcgggctgg cgcaccaccg taagagtagg gtgcagaatg
    19501 tcggcaagct caaaatcatc aaagcccgcc agggccactt cttccggcac ctttacgcca
    19561 tgatgaatca atgcatgaag cgcgtagcgc gttgtcaggt tgtttgccgt aaaaaacgcc
    19621 gtcgcgggct ccctgttttg cagcatccca atgatgatct cggctgttgc ttcttctgag
    19681 gagcagtcaa aagtaggttc cggttcaaga ccggcttcca gcatggccct tcggtatccc
    19741 tcgaagcgcg cccggacggt gtagaggttc ttgttataac caagaaatgt gatgcgcttg
    19801 tgcccatgct gctgaatcag atgctgcacc gcccgctttg ccccgctctg gttctgcacc
    19861 tggactgaat ctatgcggtc gtcgtggaca gggcggtcca tggtgacgat atgcaccccg
    19921 ccgaactctg actggttgat ccgggacttg cgtatgtcgg caggaatgat caccatgccc
    19981 tctacgtggc gttgcaccat agattgcgcc tcgtcgtact ccgctgccgg atcgttgttg
    20041 gatgtagtga gcatcacgga gtagccctgg tcgcgcgcca cggtattcac agcatgggcg
    20101 caggtggcaa aaaactggtc atagagataa ggaacaatca gtccaatggt gcgggacttc
    20161 aggctccgca acgcgcgcgc catctcgttg gggcgatagc caagcttctc aatcgcgcga
    20221 tatacccgtt tggccgtctc ttctgtgacg ttggctgacc cgttcagcac gcgcgatacg
    20281 gtcatcgttc cgacgcctgc aacccgcgca acatcgttca tcgtaggctt gcggtccata
    20341 aactatgacc atcgcattcg cctctggggc gcgtcaataa agaagacaca tctttcgcaa
    20401 aaatgccata aacttccatt tgaaggaatg cggcgcctct gcttcatcat tgcttttctg
    20461 ctttgtctgc cggcctatgc gcaggacact ctctatgcca aaggccgcgc cgcctttgac
    20521 accagtcaat ggaacgaggc cgcatcgctg ttggcgcagg ccgaagctgc ttctccagga
    20581 acgacagatg cactgttgta tgaagccaaa gcgctgctct atctgggccg tcttgccgaa
    20641 gcagaaaaga ccctcaatag ctatctcaca cggcatcccg gttccgccga tgccctctac
    20701 gcgcttggtc ttgtccagca gcgtgaaaat cacccgcgtg attctctcgc cacaatgacg
    20761 cacgcggcgc aactgcgcac accggcatcg gaagacctgc gcatcgtggg gctcgattac
    20821 gtcctgctga acgactaccc cgacgcaatt cactggctgg aaaaggccgt cgagttcgac
    20881 agcaaaaaca cggaagcatg gtacgcgctc ggtcgcgctg attacacgca gagccgcttt
    20941 cacgatgcag agcacgcatt tcagcaggcg cttctgctcg atccggaaca catcagggcc
    21001 acagaaaacc tcggcctggt ctacagtgcg gaaaaccgcc tgcccgatgc cgatgagacc
    21061 ttccgcaagg ccgtaatgct tgctgaaaag gacggccata cagatgaatg gccatatctg
    21121 aattacggca gctttctgct ggaccagggc cgcgcatccg aagccattcc actgctggaa
    21181 cgtgcaacga gtatcaatcc aaagtgcgct gcgtgtttcg aaaagctggg ccgcgcgctc
    21241 agcgcaacag gaaaattgca ggaaggcgtg gagcatctgg aaaaggccgt ctcccttgat
    21301 ccaa
//
LOCUS       DASRBV010000330        13117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_537651, whole genome shotgun sequence.
ACCESSION   DASRBV010000330 DASRBV010000000
VERSION     DASRBV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537651"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFA02_12285"
     CDS             complement(<1..990)
                     /locus_tag="VFA02_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10672.1"
                     /translation="MSFWSRIWNAVHGERLNREIEEELQSHMEEAIASGRDPEEARRA
                     FGPPLRMYEASHGIRAAQWLESLLADVRFGWRQLCRNKITSAAAVLSLALGMGSCIAA
                     FRLMDALFWRPLPISHASRLYVLSRKMIGLYGKPIEDGHWATSEFNIMRDAVRGQANL
                     VAISDADRTDITWSTDDAMEKAHVVYVSGNMFPLFGLKPALGRLLTPADDRGLGLGPY
                     AVLSWDYWDRRFGRDPNILGRPLHINSEIFEIIGIGPREFTGTEKGTVADIFLPLNMN
                     KWATQENFDWHRVFLMLKPGVDPATALGPLSQHLSAVNHAFEAGCSTCFRGATQ"
     gene            complement(992..1321)
                     /locus_tag="VFA02_12290"
     CDS             complement(992..1321)
                     /locus_tag="VFA02_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU10673.1"
                     /translation="MARTDALQGSLDLLVLKILSRRPRLHGYAIMAAIASTSGDVLRA
                     EEGSLYPALYRMEEAGWIRAEWIRKENGRRARVYELTAAGKKQLGLEESRWQSVSLAI
                     NRVLREA"
     gene            complement(1359..1766)
                     /locus_tag="VFA02_12295"
     CDS             complement(1359..1766)
                     /locus_tag="VFA02_12295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZU10674.1"
                     /translation="MVAIQLEYQGDLHCKAVHGPSGTELTTDAPKDNQGRGESFSPTD
                     LVATALGSCMLTVMGIMARTLNIDITGTTATVEKEMATTPVRMIRSLSVKIHVPHQVS
                     EEDRKKLERAAHTCPVHKSLHPDVEKPIAFTWG"
     gene            1943..2455
                     /locus_tag="VFA02_12300"
     CDS             1943..2455
                     /locus_tag="VFA02_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opioid growth factor receptor-related protein"
                     /protein_id="HZU10675.1"
                     /translation="MGAAAILEFYRGGCDDEGRMLEDILSWPDEELEAVHNFIQWLFP
                     LPERSGANPGAPVLDEATIEAFHRSPELQGRLRQAFLRMMRFYGFVWRGGQVVPSEDF
                     SQKAQNWLHPDNHNHLRLTRILRSLRILGLETEMQALFAALKEVYNGHSGSITSRTFD
                     FWTRAADVSS"
     gene            2532..4235
                     /locus_tag="VFA02_12305"
     CDS             2532..4235
                     /locus_tag="VFA02_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU10676.1"
                     /translation="MLNFVQDEAADQHYSLAFQAASEGFWQWDLRAGIMHYSSRWQAI
                     LGEEAAEFSGTVEHWLGRVHPEDRPRLEAELKALGAGKSAGFQYEHRIRHRDGSWRWV
                     VARGLADRGRDMAGGALADHTERRTCDPLTGLPNRLSFLERLERRLREAHGQKVWNFA
                     VVSVNLERYRLVSESMGFAAGDSLLIEAARRTANALKGGHFAARVSDTEFSVLLEDTR
                     NKLEALQMASAIQQEMAQPFVLRSKKINIAAWTGVAIADASYQRVEDLLRDASLAMER
                     AKSSGRPRPVCFSVTMRESALRKLQLEAELRNAIEAGEMVLYYQPEVDLQSGRVVGLE
                     ALVRWQHPERGLVPPSDFIPIAEETGLILPLGDWVLKEVCRQMAAWRSLAGDLLDLRV
                     SVNLSAKQFSQPDLVERIAAILEDAGVAARHVGLEMTESSLMADSETAFKTMQQLRRM
                     GVGLHMDDFGTGYSSLNHLHRFPFDTLKIDRSFVQRMISEKGPQEIVSAIVHLARSLK
                     MHVVAEGIENASQMQFLQSLGCHFGQGYYFAKPLPASTINAILESQAAGQDPVMLRFV
                     N"
     gene            complement(4315..5079)
                     /locus_tag="VFA02_12310"
     CDS             complement(4315..5079)
                     /locus_tag="VFA02_12310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZU10677.1"
                     /translation="MNLTPVVLQPNPASSLEVQEAIKLQPASVIPHGKAFVHQALDEN
                     AWSLSDGKRLFDFVAATIGLVLFFPLMVLIGVAIAVTSPGPVLFRQKRMGRRGTLFTI
                     YKFRTMVDGAEKGPCITVEGDTRITRIGAILRKFKFDELPQFINVIKGDMSFVGPRPK
                     LPHHESLHMPFRPGITGAATLAFRCEEELLRRIPAQDLDFFYARTVKPLKARLDWEYM
                     QRATWWSDLGLLWCTLTACLSRQVVPAPVIEFPGGD"
     gene            complement(5459..8254)
                     /locus_tag="VFA02_12315"
     CDS             complement(5459..8254)
                     /locus_tag="VFA02_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZU10678.1"
                     /translation="MHKRLVLSLAVILLAAMPHHARTQTTTDSLSQNQGQQGQATDCS
                     DPANAMSAACSSANQGAYGLGSNLQTGESNVPAGSPLTMRTYTDLGIGATGTTAATQQ
                     RTGLVPSTVSAPPEPLTEFQKFVAGTTGEVLPIFGADLFRYVPTTFTPLEQIPVTPNY
                     IIGPGDVLRIRVWGQINFNADVRVDRTGSIYLPQVGTIHVGGLPFSALDQHLRSEIGR
                     VYRNFDLTVDVGQTRSIQVYVVGQARRPGAYTVSALSTLVNALFSSGGPSSQGSLRDI
                     QLKRNGQTVTHFDLYDLLVNGDKTKDVQLQSGDVVFIPAAGPQVALTGSVRRPGIYEL
                     QDEKTTIQQLLAYAGGASAIAADTRISVDRVAAGHAREAMEVSFDSTGLGTTLRDGDV
                     LRVLSIPAKYEKTITLRGNTANPGRFAWHEGMKLSDLIPDKDSLITRDYYWRRAQLGL
                     PTPEFLPYIAGPVQYQPSHPIDLQSRQAYQQWQMQNRFQRLPNGQYAVIAPNPAQQGQ
                     PGENKSAANEYPYAQGSVFLQGEPQYTQVLPQTQQQAGQEGISTPADLYGTGQTGQLQ
                     QPAGTMNPMQGGVLNPSQLSSSGSLGGRQVITQNTASATRKNDVRLSAPEIDWDYAVI
                     ERMDPQTLKTSLLPFNLGKLVLNHDPSQDLALQPGDVVTIFSQADLRVPQERQTKFVR
                     LEGEFASSGIYSVRPGETLRQLVIRAGGLMPDAYLYGSEFTRESTRVLQQQRLDEYVQ
                     TVELETQRGVLAGTSSAASAQDVASAQAAQASARDLLSRLHQLRATGRIVLQIKPDAT
                     GLDGLPDLPLEDGDVFIVPPRPSTVNVVGAVYDQNSFMFTPGRRVGDYLKMAGGPNRD
                     ADKKHMFIIRADGSVVSRTVNSGVWGNTFESARIYPGDTIVVPEKTFRPTILRGILDW
                     SQLFSQLALGAAAISVI"
     gene            complement(8390..8749)
                     /locus_tag="VFA02_12320"
     CDS             complement(8390..8749)
                     /locus_tag="VFA02_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZU10679.1"
                     /translation="MNQFRRYFFAILLTVLLVPCAMQAQTERTITIHAKRYKFVPAEI
                     TLVKGETVRLVLISDDVDHGLAVKGLGIRADMPKHKRVEVTVTPEQTGDFPGICSRFC
                     GAGHGSMKFVVHVVEKQ"
     gene            8936..9922
                     /locus_tag="VFA02_12325"
     CDS             8936..9922
                     /locus_tag="VFA02_12325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU10680.1"
                     /translation="MQQRRLGRQGLRVSAIGLGCMGMSDFYGQRNESESIATIHRALD
                     LGINFFDTADIYGPHTNEELVGRALKPYRDKVILATKFGIIRNPDGSRSVNGRPEYVR
                     AACDASLKRLGIDMIDLYYQHRVDPNTPIEETVGAMAELVKAGKVRYLGLSEAAAQTI
                     RRAHAVHPITALQSEYSLWSRDHEDDGVLPTIRELGIGLVAYSPLGRGFLTGQIRTPS
                     DLGPDDWRHSSPRFQGENFQKNLDLVAKIEELAEKKKVKASQLALAWVLAQGEDIVPI
                     PGTKRRNYLEENVAAAEIELTEEDLEALDAIAPLGAAAGERYAPAAMKLVNL"
     gene            10132..11877
                     /locus_tag="VFA02_12330"
     CDS             10132..11877
                     /locus_tag="VFA02_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU10681.1"
                     /translation="MKQFGCFQIDTQNEMLWCDGKQVPLAPKPFAVLRYLVENAQRLV
                     THDELLDALWPETYVQPQVLRTYILELRKVLGDDPDEPQYIVTVPKRGYRFVCKVEEV
                     HEASASGTGPEVCIVGRQAELTKLEGDLERAARGQRQLVFLSGDAGIGKTALVDAFCH
                     RAGSNSKLRIARGQAVEGFGGKEAFYPVREALGQLLAYEKTSQLLARLLPAWFDKKTD
                     APAKAPSLGELCEAVEIIAHDEPLLLVFEDLHWADTSTLDFISALARRRGAAKLMVLA
                     SCRPGEMDGQHPLKRLKEDLTTHRLCCVIPLGLLNREAVANYLTRQLKTSALPSGLPS
                     FVHQHSEGNPLFMAATLEHLQTQRFLENKNGVWQLAVPLAEIELGIPHGLLGMIDLQL
                     ERLSEADRRLLEAGSIVGAIFPAWAAAAALKTGVPDVEDEYAALARRTRLINPAGQDE
                     LPDGTRSTFYVFAHGLFREALYSRQPAARRAERHRRVAERLKAMFAGSEASIASELAR
                     HYEAAGCWKQAIEELQLAAETAAKREAEHAACELLKRALDLSANLKAEERKTIERVLR
                     EKLRKYSEWVSMEIA"
     gene            11992..12315
                     /locus_tag="VFA02_12335"
     CDS             11992..12315
                     /locus_tag="VFA02_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU10682.1"
                     /translation="MNTYLQSGCEALGDPTRFAIFSALAERPQAVGELAALFPVSRPA
                     VSQHLKVLKNAGLVTDRKSGTRRVYAVDPEGVARLRKELDRIWNDALTAFKRAVETWE
                     EPHNV"
     gene            12308..12790
                     /locus_tag="VFA02_12340"
     CDS             12308..12790
                     /locus_tag="VFA02_12340"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU10683.1"
                     /translation="MSETLAPVVKTVTVNASREKAFRIFTEFFGGWWPSTHHIGKHPF
                     ETAILEGRVGGRWYERMTNGEECNWGHVKVWDPPARVVLSWHVQPDWGYDPDPEKASE
                     VEVRFIPESETVTRVELQHRNIERHGALAGKLREGVSDGWPTVMDLYARFANEQAGEK
                     "
     gene            12790..>13117
                     /locus_tag="VFA02_12345"
     CDS             12790..>13117
                     /locus_tag="VFA02_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10684.1"
                     /translation="MKTVDRIFGWLLLIGGLLHGMGSLQGYGKNHEMLLWALCASLAV
                     LLLAAINLLRVGRPQDRALAWISFLGCIGWLVAAIAFGRLIGNIFDFRPLTHEIITIV
                     LAIFSLR"
BASE COUNT         2819 a         3664 c         3852 g         2782 t
ORIGIN      
        1 ctgggtcgcg ccgcggaaac acgtagagca tccggcctca aaggcgtgat tgactgctga
       61 gaggtgctga ctaagcggcc caagcgcagt ggccggatca acgcccggtt tcagcatcag
      121 gaaaacacgg tgccaatcga aattttcctg ggtcgcccat ttgttcatgt tcaggggcag
      181 aaagatgtct gccaccgttc ccttttcggt gcccgtgaac tcgcgagggc caattccgat
      241 aatttcgaaa atctcgctgt taatgtggag agggcggccg aggatgttgg gatcgcggcc
      301 gaaacggcgg tcccagtagt cccaggagag gaccgcataa ggcccaaggc cgaggccacg
      361 gtcgtcggca ggagtaagca gacggccgag cgctggcttg agaccgaaga gcggaaacat
      421 attgccggag acataaacga cgtgtgcctt ctccatagca tcgtcggtag accaggtgat
      481 gtctgtgcgg tccgcgtcgc tgatggcgac aaggtttgcc tggcccctga cggcatcgcg
      541 cataatgttg aactccgacg tggcccagtg gccgtcctcg atgggtttgc catacaagcc
      601 aatcatcttg cgggaaagga cataaagcct gctcgcatgg gagatgggca gcgggcgcca
      661 gaacagcgca tccatcaggc ggaaagctgc gatgcaggag cccatgccaa gcgcaagcga
      721 aagcacagca gcagccgatg taatcttatt gcggcaaagc tgccgccagc caaaacgtac
      781 atctgcgaga agcgactcaa gccattgggc agcacggatg ccgtggctag cctcgtacat
      841 gcgcagcgga ggaccgaacg cgcgccgcgc ttcctcggga tcgcgtccgg aggcaatcgc
      901 ttcctccata tgcgactgca actcttcctc aatttcgcgg ttgaggcgtt caccatggac
      961 ggcattccag atacgcgacc aaaaggacat atcatgcctc ccgaaggacc cggttgatgg
     1021 ccaagctgac ggactgccag cgagattctt ccaggcccag ttgctttttg cctgcggcag
     1081 tcagttcgta aacgcgtgcc ctgcggccgt tctctttccg gatccactca gcgcgaatcc
     1141 agcccgcttc ctccatgcgg tacaacgccg ggtaaagcga gccttcttcg gcgcgcagta
     1201 cgtcgcccga tgtgcttgcg atagcagcca taatggcgta cccgtgaagc cgcgggcgtc
     1261 gcgacagtat cttgagcacg agcagatcga gtgagccttg cagtgcgtcg gtcctggcca
     1321 tgttcggatg cctctctgca ggtcaatctg gggttttgtc atccccatgt aaatgcaatg
     1381 ggcttctcca cgtccgggtg caggctctta tgcacaggac aggtatgggc cgccctttcc
     1441 agttttttcc ggtcttcttc actgacctga tgaggcacgt gaattttgac agacaggctg
     1501 cgaatcatcc tcaccggggt tgtggccatt tccttttcca ccgtggccgt agtccccgtg
     1561 atgtcaatgt ttaaagtgcg cgccatgatg cccatgacgg tcagcatgca gcttccgagg
     1621 gcggtggcca ccaggtcggt tggtgaaaag ctttctccgc ggccctgatt gtctttcggc
     1681 gcgtcggtgg tcagctccgt cccggatgga ccatgtacag ctttgcaatg taagtctccc
     1741 tggtactcca actggatagc aaccattgtg ttcctttcgg caaatatata gacatcttta
     1801 tatagacatc taaataaaga tgtcaatggg ccgtagcgca ttttgaaaga aaccggaacc
     1861 ataccggtgc cgcaagctgt gtttctctct caccgcctgt tatggcgcgc ggcatccttc
     1921 gcctgtaagc atcaaaatgt ttatgggtgc tgcagcgatt ctcgagttct accgcggcgg
     1981 gtgtgatgat gaaggccgca tgctggagga catcctttcg tggccggatg aagagcttga
     2041 ggccgtccac aattttattc agtggctctt tccgcttccg gaaaggagcg gcgcaaaccc
     2101 cggcgctccg gtgctggacg aggccacaat agaagcgttc caccgctctc cggaattaca
     2161 ggggcgtctg cggcaggcgt ttctgcgcat gatgcgcttt tatggatttg tctggcgcgg
     2221 cggccaggta gttccctcag aggatttttc ccaaaaggca cagaactggc tccatccaga
     2281 caatcataac catctgcgtt tgacgcgcat tttgcgctcg ttgcgaattc tggggctgga
     2341 gacggagatg caggcgctct ttgcggcgct gaaagaggtg tataacggcc attccgggag
     2401 catcacctca cgcaccttcg atttctggac gcgcgcggca gatgtttcgt cataaggtac
     2461 ctcttcgtct ttcctcgcta aacgtctttc ttccttctgc cgataggcag agtggaggcg
     2521 cccgcgtcat catgctcaat tttgttcagg acgaggctgc ggatcagcac tactcgctcg
     2581 cgttccaggc cgcatccgaa gggttctggc agtgggacct gcgcgctgga atcatgcatt
     2641 actcgtcgcg ctggcaggcg attctgggcg aggaggccgc ggagttttcc ggcacggtcg
     2701 aacactggct tggccgggtg catcctgaag acaggccgcg gctggaagcc gagctgaagg
     2761 cccttggcgc aggaaaatcg gcaggcttcc agtacgagca ccgcattcgt caccgcgatg
     2821 gaagctggcg ctgggttgtg gcgcgcggcc ttgcggacag aggcagagat atggccggcg
     2881 gcgcgctggc cgaccatacc gaacgaagga cgtgtgatcc gctgacgggc cttcctaacc
     2941 gtctctcttt tctggaacgg ctggaacggc ggctgcgtga agcgcatggg cagaaggtgt
     3001 ggaactttgc cgttgtttcc gtcaatctgg agcggtacag gctggtttca gaaagcatgg
     3061 ggtttgccgc cggagactct ctgctgattg aagcggcgcg acgcacggcc aatgccctga
     3121 aaggcggcca ctttgccgca cgcgtctcgg acacggaatt ttctgtcctg ctcgaagata
     3181 ccaggaacaa gctggaggcg ctccagatgg cctcggcgat ccagcaggag atggcccaac
     3241 cctttgtctt gcggagcaaa aagatcaata ttgctgcctg gacgggagtg gccatcgcgg
     3301 atgcgagcta tcaacgtgtg gaagacctgc ttcgggacgc gagtcttgcg atggagcggg
     3361 caaaatccag tggccgtccg cgcccggtct gtttttcagt caccatgcgc gagagtgcgc
     3421 tgcgtaagct tcagttggaa gccgaactgc gcaatgccat cgaagcgggc gaaatggtcc
     3481 tctactatca gcccgaggtg gacctgcaat cgggccgcgt cgttggcctg gaggcgctgg
     3541 tgcggtggca gcatccggag cgcggcctgg ttcccccgtc cgatttcatc cccattgcag
     3601 aagagacagg attgattctt cccctgggcg actgggtact gaaagaggtg tgccgccaga
     3661 tggctgcgtg gcgttcgctt gccggagacc tgctcgacct gcgggtgagc gtcaacctct
     3721 ctgcgaagca gttcagccag cccgatctgg tagagcgcat cgccgccatt ctggaagacg
     3781 ccggagtggc cgcccgtcat gtgggccttg aaatgacgga aagcagtctg atggctgatt
     3841 ccgaaactgc attcaagacc atgcaacaac tgcgccgtat gggcgtgggc ctgcacatgg
     3901 acgatttcgg caccggctac agttcactca accacctgca ccgctttccc tttgacactc
     3961 tgaagattga ccgctccttc gttcagcgga tgatcagcga aaaggggccg caggagattg
     4021 tcagcgctat cgtccatctg gcgcgctcct taaagatgca tgtcgtggcc gagggcatcg
     4081 aaaacgccag ccagatgcag tttctgcaat cgctgggctg tcatttcgga caaggctatt
     4141 actttgcgaa accgctgccg gccagtacca tcaacgccat cctcgagtcg caggctgcgg
     4201 ggcaggaccc agtgatgctg cggttcgtga actaacgctc gtgagacatg cagcttctct
     4261 tccggttcca gaattctgcg aagactgccc actcagcgga ttttcgggac tttattagtc
     4321 cccgcccggg aactcgatga ccggagcggg gacgacctgc cgggagaggc aggcagtcag
     4381 ggtgcaccac aacaggccca gatcgctcca ccaggtcgcc cgctgcatat actcccaatc
     4441 aagcctggcc ttgagcggct tcacggtgcg cgcgtaaaag aagtccaggt cctgggcagg
     4501 gatgcgccgc agcaactctt cctcacagcg aaaggccagc gtcgctgcgc cggtaatgcc
     4561 gggccggaag ggcatgtgca ggctttcgtg gtgcggcagc ttaggacgcg gccccacaaa
     4621 gctcatgtcg cctttgatga cattgataaa ctgcggaagc tcatcgaact tgaatttgcg
     4681 aagaattgca ccaattctgg tgatccgcgt atcgccctcg acagtgatgc agggaccttt
     4741 ctcggcgccg tctaccatgg tgcggaattt atagatagtg aacagcgtcc ctcgcctgcc
     4801 catgcgcttt tgccggaaga ggaccgggcc gggagaggta acggcaattg caacaccgat
     4861 caggaccatc agcgggaaga aaagaaccaa tccaatcgta gctgccacaa agtcgaacag
     4921 gcgtttacca tccgagaggc tccaggcatt ttcatccagc gcctgatgga cgaatgcctt
     4981 gccgtgcgga atgacggacg ccggttgcag ttttatggcc tcttgcactt ccaggctgct
     5041 tgcaggatta ggctgcaaaa caacgggggt caggttcata attcgattcc tggtacggca
     5101 aattacagcg tgctcagatc ggggtagtta actatgaatt caaatttgcc aaaaagtatc
     5161 ggattgcaac ttactcttag acttaactgc aatatcgtcc gtaagtttcg aattctggaa
     5221 taatttattt ttttatgact atattgtact tcttcagggg cggcagactg aatttcacac
     5281 ccccggtttg ctgtcctgcc tcggcgccaa agcaaccatt atgctacagg ccattctctg
     5341 acttcatcaa aaatatggtc atctggcccc ggtaacgaag gtccaatgga ctccttttcg
     5401 cgttgttttc tggctgtcat tagtgggacg caatcaagcc gtccctgggt tttccctttc
     5461 aaattacgct gatggcagcc gccccaagcg caagctggga gaagagctgc gaccagtcaa
     5521 gaattccgcg caggatcgtt ggccggaagg tcttctccgg caccacaatc gtgtcgcccg
     5581 ggtaaatcct ggctgattca aaggtatttc cccatacgcc actgttgacg gtgcggctta
     5641 cgaccgaccc gtcggcccgg atgataaaca tatgcttctt gtccgcatcg cgattcggcc
     5701 cccccgccat ctttagataa tcgcccacac ggcggccagg cgtgaacatg aacgaattct
     5761 ggtcatagac cgccccaacc acattcacag tggagggcct tggcggcaca ataaatacat
     5821 cgccgtcttc caaaggcaaa tcgggaaggc cgtccaggcc cgtcgcatcg ggcttgattt
     5881 gcaggacgat gcgccctgta gcgcgaagct ggtgcaggcg ggaaagcagg tcccgcgcgc
     5941 ttgcctgggc cgcctgcgcg ctggccacgt cctgcgcgga agcagccgat gatgtcccgg
     6001 ccaggacgcc gcgctgtgtc tccagctcaa ctgtctgcac atactcatcc aggcgctgtt
     6061 gttgtaatac gcgagtggat tcgcgggtaa attcagagcc atacagatac gcgtccggca
     6121 tgagtcctcc agcacggatg acgagctgcc gcagggtctc gccgggacgg acgctgtaga
     6181 tgccggagct ggcaaattct ccctccaggc gaacgaactt tgtctgtcgc tcctggggca
     6241 cgcgcagatc ggcctgcgag aagatggtga ccacatcgcc aggttgtaag gccaggtcct
     6301 gcgacggatc gtggttgagg accagtttgc cgagattaaa cggaagcaga gaggtcttga
     6361 gcgtctgcgg gtccatgcgc tcgatgacgg cgtagtccca gtcaatttca ggagcagaga
     6421 gccgcacatc attctttcgg gttgccgatg cagtgttctg tgtaatgacc tgtctgccgc
     6481 ccagacttcc gctgctgctt aactgtgacg ggttcagcac tccgccctgc atggggttca
     6541 tggtccctgc cggctgctgt agctggccag tctgtcctgt tccataaagg tcggcaggcg
     6601 tagaaatgcc ttcctgtccg gcctgttgct gggtctgcgg aagcacctgg gtgtactgcg
     6661 gctcaccctg aagaaagaca ctgccctgcg cgtaaggata ctcattggca gccgatttgt
     6721 tctcgccagg ctgcccctgc tgcgccggat tgggtgcgat gacggcgtat tgtccattgg
     6781 gcaggcgctg aaagcggttc tgcatctgcc attgctggta cgcctgccgc gattgcagat
     6841 caatggggtg ggaaggctga tattgtaccg ggcctgcaat gtagggcaga aattccggag
     6901 taggcagacc gagttgtgcg cgccgccagt aataatcgcg cgtgatgagc gagtctttat
     6961 caggaataag atcgctcaac ttcattcctt catgccatgc gaagcgtccc gggttggcgg
     7021 tgtttccgcg cagcgtaatg gttttttcgt acttcgccgg aatggaaagc actcgcagga
     7081 catcgccgtc gcgcagagtt gtgcccagtc cggtggagtc aaaactgact tccatggcct
     7141 cgcgggcatg tccggcggca acgcggtcta ctgagattcg ggtgtctgcg gcaatggcgg
     7201 acgcgcctcc ggcgtaggca agcaattgct ggatggtggt cttctcatcc tgtaattcat
     7261 aaatgcccgg acgccgtacg ctgccagtga gggcgacctg cggcccggcg gccggaataa
     7321 agacgacatc gcctgactgg agctgcacgt ctttggtttt gtcaccattt accagcaggt
     7381 catacaggtc aaagtgggtg acggtctgtc cattgcgctt gagctgaatg tcgcgcagag
     7441 acccctggct tgatgggcca cccgaagaaa agagggcgtt caccagcgtg ctcaaagcgc
     7501 tgacggtata tgcgcccgga cgccgcgcct gccccacaac ataaacctgg atggagcggg
     7561 tctgccccac atccacggta aggtcaaaat tgcggtatac acggccaatt tcagagcgca
     7621 gatgctggtc cagagcagaa aacggcaaac cgcctacgtg gatggtccct acctgtggca
     7681 aataaatgga gcctgtgcgg tctacgcgca catcggcatt gaaattaatc tggccccaga
     7741 cgcgaatgcg gagcacatct cccgggccga tgatgtagtt cggggtaacg ggtatctgtt
     7801 caagcggagt aaaggtggta ggcacatagc gaaacaggtc cgcgccaaag atgggcagca
     7861 cctcgcctgt ggtcccggca acaaatttct gaaactcggt aagcggctcg ggaggagcag
     7921 acactgtgga aggcaccaga ccggtccgct gctgggttgc tgctgtggtt cctgtggcgc
     7981 cgatgccgag atcggtatac gtgcgcatcg tcagaggcga acccgccggg acattgctct
     8041 ctccggtctg aagattgctc cctaatccat aggcgccctg gttcgcgctg ctgcatgccg
     8101 ctgacatggc atttgccgga tcggagcaat ccgttgcctg tccctgctgt ccctggttct
     8161 gtgagagaga atccgttgtg gtttgggtac gggcatgatg cggcatggct gccagcagga
     8221 tcacggcgag agaaaggacc agtcgcttat gcataacaca tgcaacctgc acgttttatc
     8281 aggctgcgca ttcactcctt taaccggctt gcttgttctt cagttgaaag tgtagagcct
     8341 gcggggtacg aaagaaacca ctttacaggc cacaccattg atgaaagagc tactgctttt
     8401 caactacatg gacaacgaac ttcatggatc cgtgcccagc gccgcaaaac cgcgagcaga
     8461 ttcccgggaa atcgcctgtc tgctccgggg tgaccgtcac ttctacacgc ttgtgctttg
     8521 gcatgtctgc gcggatgccc aggcctttta cggccagccc gtggtccacg tcgtcggaga
     8581 taagcaccag cctgaccgtc tcacctttta ccagagtgat ctctgcagga acaaatttgt
     8641 agcgtttggc gtgaatggta atggtgcgct ctgtctgtgc ctgcatggcg caggggacga
     8701 gcaaaacagt caacaggata gcaaagaaat agcggcgaaa ctgattcaca aatccaaaac
     8761 cttattttct tctattgtac agacgcgcgc cgcctggaag aggcacaacc ctggcccccg
     8821 catacagatc cactcaacga gaaaaaagca ttgtccgccc aatagataaa actgttcacc
     8881 agcccgcatc cccgaacata cagacgaatc tgatagttca tagggagaga accacatgca
     8941 gcagcgcaga ttaggacggc aggggcttcg cgtttctgcc atagggttgg gatgcatggg
     9001 catgtccgat ttttatggcc aacggaacga gtccgagtca attgcaacca ttcaccgcgc
     9061 gctggacctc ggtatcaact tcttcgatac agccgacatt tacggtccgc acacgaatga
     9121 ggagctggtt ggccgcgcat tgaagccata ccgcgacaaa gtcattcttg ccacgaaatt
     9181 tgggatcatt cgaaatcctg atggatctcg aagcgtaaac gggcggccgg aatatgtccg
     9241 tgcggcctgc gatgcaagcc tcaagcggct gggcattgat atgattgacc tttactatca
     9301 gcaccgcgta gacccgaata ctccgattga ggagaccgtc ggggcaatgg cggaactggt
     9361 gaaggcaggt aaggtgcgct atcttggact gtctgaagcc gctgcgcaga cgatccgcag
     9421 agcgcacgcc gtgcatccca tcaccgcgct ccagagcgag tactcgctgt ggagccgcga
     9481 ccatgaggat gatggagttt tgcccacaat tcgggaactt ggcataggtc tggttgcgta
     9541 ttctcctttg ggaaggggct tccttaccgg acagattcgc acccccagcg acctggggcc
     9601 ggatgactgg cgacacagtt cgccccgttt tcagggagag aacttccaga aaaatctcga
     9661 tttagtagcg aaaattgagg agcttgccga aaagaaaaag gtgaaggcgt cacagcttgc
     9721 tctggcatgg gtcctggcac agggcgagga cattgttccg attcccggca caaaacgccg
     9781 caactatctg gaagaaaacg tggctgctgc ggagatcgaa ttaacagagg aagaccttga
     9841 ggcgctcgat gcgattgctc ctcttggagc ggcggcagga gaacggtatg ctccggccgc
     9901 aatgaaactt gtaaatctgt aacccgccgt ccggaaatgg acatccagtg tccatttccg
     9961 gacataatcc tgagcatgac gtactttcca tgcgcttaac ccgcatagaa tcatcggcaa
    10021 tttttgagtg gcggctggca cgtggcttgc taacagttta tagataaaca aagtcccagc
    10081 taataacgtt taaaagaatc aggccggcaa aaaaggtcgc tgcaagggtt tatgaagcag
    10141 tttggttgct tccagataga cacacaaaac gagatgcttt ggtgcgacgg caaacaggta
    10201 ccgctcgctc caaagccgtt tgccgtgctg cgctatctgg tggaaaatgc gcagcgcctg
    10261 gtgacacatg acgaactgct ggacgcgctg tggccggaaa cctatgtcca gccgcaggta
    10321 ctgcgcacct acattctgga actacgcaag gtgctcggcg atgatccgga cgaaccgcag
    10381 tacatcgtca cggttcctaa acgcggatat cgtttcgtct gcaaagtgga agaggtacac
    10441 gaggcttccg catcaggcac tgggccggaa gtatgtattg tgggccggca ggcggagctg
    10501 acaaagctcg aaggcgatct ggagcgggca gcccgaggcc agcggcaact tgtctttctt
    10561 tccggagacg caggcattgg caaaacggcc ctggtggatg ccttttgtca tcgggccggc
    10621 agcaatagca aactacgtat cgcccgcggg caggctgtag aaggcttcgg cggcaaagag
    10681 gcgttctatc ccgttcgtga agcgctgggc cagttgcttg cgtatgaaaa gacgagccag
    10741 cttctggcaa gactgcttcc ggcatggttc gataaaaaga cagacgctcc ggccaaagct
    10801 ccttcgttag gggagttatg tgaggcggtc gagatcatcg cacatgatga gccgctgctg
    10861 ctcgtctttg aagacttgca ctgggcggat acatccacac tcgacttcat ctcggcactg
    10921 gcgcgcagac gcggagcggc aaaactgatg gtattggcaa gctgccgccc cggagagatg
    10981 gacggccagc acccactgaa gaggctgaag gaagacctga ccacgcaccg cctctgttgc
    11041 gtcattccgc ttggcctgct caaccgtgag gcagtagcga actatctgac ccgccagctt
    11101 aagacttctg ccctgccatc cggactgccc agctttgttc atcagcactc tgagggcaat
    11161 ccgctgttca tggctgcgac cctggaacac ctccagacgc agcgttttct cgaaaacaaa
    11221 aatggcgtat ggcaattggc cgttcctctg gcagaaatcg aattgggcat tccgcacggc
    11281 cttctcggca tgattgatct gcaactggag cggctttcag aagctgaccg ccgcctgctc
    11341 gaagccggaa gcatcgtcgg tgcaatcttt cctgcctggg ccgcggctgc tgcgctcaaa
    11401 accggagttc ctgacgtcga agacgaatat gccgcactcg cgcgcaggac gcgactcatt
    11461 aatcccgcag gacaggatga actgcccgac ggcacccgct ccaccttcta tgtatttgcg
    11521 catggactct tccgcgaggc gctctattcg cgccagcctg cggcacgccg ggccgaacgt
    11581 catcgccggg ttgcagaacg gctcaaggcc atgtttgccg gaagcgaagc cagcatcgcc
    11641 tcagagcttg cccgccatta cgaggccgcc ggatgctgga agcaagccat cgaagagttg
    11701 cagctcgccg ctgaaaccgc cgcaaaacgc gaagctgaac acgccgcctg cgaactgctg
    11761 aagcgcgccc tcgacctgag cgcaaacctg aaagccgaag aacgcaaaac catcgagcgt
    11821 gttctgagag aaaaactgag aaagtactct gaatgggtct cgatggaaat tgcctgattg
    11881 aagaaattcc atctttgaga ttgtttgccg aatgggcctt tcactcacac gccattgacg
    11941 ctgactgtcc tgcttgacaa ccacgcatct gtaagtattt acttaccgta agtgaatact
    12001 taccttcagt ccggatgcga agcgctcgga gaccccacgc gctttgccat cttcagcgct
    12061 ttggcggaac gtccccaggc ggtcggagag ctggctgccc tgtttccggt gagccgtccc
    12121 gcagtttcgc aacacctgaa ggtattgaaa aatgccggat tggttacaga ccgcaaatcc
    12181 ggaacgcgtc gcgtgtatgc cgttgacccg gagggcgtgg cccgtctccg aaaagagctg
    12241 gacagaatat ggaatgacgc tttgacagcc ttcaaaaggg cggtagaaac atgggaggaa
    12301 ccgcacaatg tctgaaaccc ttgctccggt cgtaaagacc gtgaccgtga atgcttcgcg
    12361 cgaaaaggcc tttcgcatct tcaccgaatt cttcggagga tggtggccct cgacacatca
    12421 catcggcaag catccgtttg aaacggccat tctggaaggc cgggtgggcg gccgctggta
    12481 cgagcgcatg accaatggcg aggagtgcaa ctggggccac gtgaaggtgt gggaccctcc
    12541 cgcacgtgta gtgctttcct ggcacgtgca gcctgactgg ggctacgatc ccgatccgga
    12601 gaaggcgagc gaggttgagg tgcgcttcat tccagaatca gaaacggtca cgcgcgtgga
    12661 actgcagcac cgcaacatcg agcggcatgg cgcacttgcc gggaaactgc gcgaaggcgt
    12721 cagcgatggc tggccgacgg tcatggatct gtacgccagg tttgccaatg agcaggcagg
    12781 agagaaatag tgaaaactgt tgaccggatc ttcggatggc ttctgctgat cggagggctg
    12841 ctgcacggaa tgggcagcct gcagggatac ggcaaaaacc acgagatgct cctctgggcg
    12901 ttgtgcgcat cgctggccgt gcttctgctg gcagcaatca accttctgcg cgtgggacgc
    12961 ccgcaggacc gcgcactcgc ctggataagt tttctcggct gtattggatg gcttgtcgct
    13021 gcaatcgctt ttggacgatt gattggaaac atctttgact ttcgcccgct cacacacgaa
    13081 atcattacca tcgtgctggc gatcttcagc ttacgta
//
LOCUS       DASRBV010000331         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_429241, whole genome shotgun sequence.
ACCESSION   DASRBV010000331 DASRBV010000000
VERSION     DASRBV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429241"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFA02_12350"
     CDS             complement(<1..236)
                     /locus_tag="VFA02_12350"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZU10685.1"
                     /translation="MSLLRAVEIEEKLKHLQGWSQQGQEIVREFTFGNFVKAMQFVNM
                     VAEQAEAAGHHPDIDIRYNKVKLALVSHDAGGLT"
     gene            complement(263..1360)
                     /locus_tag="VFA02_12355"
     CDS             complement(263..1360)
                     /locus_tag="VFA02_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZU10686.1"
                     /translation="MASERILVVDTEQRGRAMIASTLENAHYYTVTASSGPEAIEHLR
                     LDPPYDLVLSDIMGENHGLDLLEGIRKLQPDTPVLLATDPTNARVAINALRKGAFDCL
                     LKPVEMDELLMAVRNALSYRRSLLQSSQQPSSLEQVIATRTEMLRRAVADLERSYDVT
                     LEALGDALDLKDAETEGHSKRVTAYTVALARAMGLAPSEIRIIARGAFLHDIGKMAIP
                     DAILLKPGKLTPDEQALMREHCVRGYQILSKIPYLREAAEIVYTHQEHYDGSGYPRGL
                     KGNEIPLGSRIFAVADTLDAITSDRPYRRAQSIEIARREIQRCSGTQFDPRVVDVYLS
                     MPDRLWMELRREISQHPRFSSVSLSTPEMKF"
     gene            1801..2331
                     /locus_tag="VFA02_12360"
     CDS             1801..2331
                     /locus_tag="VFA02_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU10687.1"
                     /translation="MTIDFRGLFQQTKPDVVAVRAEERRTQHVQRDIYDSHRHRIFSV
                     AFYMTGNEVHAEEILTNTFVQAFTVAPRPGRHAIDRALLSQLRSRFQISPVSTAVTVP
                     ESGQHLEKNILRTHLEEALQHLPPVERLLFLLRDVEGYPLEEVSSLTEIPPGEASRIL
                     MSARIRLRSILADMQS"
     gene            complement(2332..3744)
                     /gene="glmU"
                     /locus_tag="VFA02_12365"
     CDS             complement(2332..3744)
                     /gene="glmU"
                     /locus_tag="VFA02_12365"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZU10688.1"
                     /translation="MRIAIVIMAAGKGTRLKSKRAKVLHQIGGKALVEHVIHAAQQIV
                     PSHDIFVVVGHQAGAVQSEMAHTGVNFVIQAEQRGTGHAIQCARQAVQGYEHILVLSG
                     DVPLLDAKTIAQLRDFHIAEHAAMTILTAQPEDPFGYGRVLRKSAHSPEVAAIVEQKA
                     LTPQQEKIREINSGIYAFATKPLFDHINELTTNNAHGEYYLTDMARILVEEGQRVVAL
                     EAPSATEVLGANTLAELAALDAEMRMATARRLMANGVTIFRPETTVIDAGVEVAPDSI
                     LEPFVQLLGHTRIGEGCRIRSYSVIENSTLENHVLVRQGCIISESHIRSGALLGPYAH
                     LRPGSEIGEEAHIGNFVETKKIRMGKGSKANHLSYLGDAEIGSGVNIGAGTITCNYDG
                     INKHRTLIEDGVFVGSDSTLVAPIVIGRGSYIAAASCITEDVPEEALALGRARQTTKP
                     GWVTTHKGTHRPAKEVVKNS"
     gene            complement(3746..5290)
                     /locus_tag="VFA02_12370"
     CDS             complement(3746..5290)
                     /locus_tag="VFA02_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZU10689.1"
                     /translation="MIRYQRGFHSQNQRSKRPFKEAFLLLLGLTVLVALFSFTQAAFS
                     QQTQPWKQVPIPPLHPFHPQQPKRIKLPNGIVILLQEDHELPFVNGFIEMRGGSRDED
                     PGKAGLVSLYADTWRTSGTATHNGDQLDDLLEARAAKVESGGDTDSTSLSWSCLTKDE
                     DFVFNIVTDLLEHPVFREDKLQLARQQAAAAIVRRNDDAEGIAAREATKLVYGANSPY
                     ARQPELATIKAVTLDDLKKWHDRTVVGNNIIIGVSGDFNSGAMEKTLRDAFSSLPGGE
                     AVPPLKEDFSGPKPGVYYVDKNDVNQSNIYIVGLGTERSNPDFYALSVMNEIFSGGFG
                     SRLFQDVRTKRGLAYAVGGGYGAAYDHPGMFRVVAGTKSPTTIQAAEAMLEEIRDLKT
                     KPFTEDELRRAKDEVLNSFIFNYDTKEKVLAEAAKLEFYGYPADFLEKYRDSVEKVTV
                     ADVEHVAQKYIDPAKLAVLVVGNQKEFGAPLTSLNMGTPQPIDITIPGLPPQGQGGPG
                     NPEGEQ"
BASE COUNT         1191 a         1414 c         1460 g         1359 t
ORIGIN      
        1 tccgtgagtc ctccggcatc gtgagaaacc agcgcaagtt tgactttgtt ataacggatg
       61 tcaatgtccg ggtggtgacc tgcggcctca gcctgttcgg ccaccatatt gacaaactgc
      121 atggccttta caaaattccc aaaggtaaat tcacggacga tttcctggcc ctgctgactc
      181 cagccctgga gatgttttag tttctcttca atttccacag cgcgtagtaa agacattgtt
      241 tcccgctccg tgaggttttg attcaaaact tcatttccgg ggtgctcagg gaaacggaag
      301 aaaagcgcgg atgctggctg atttcccggc gaagttccat ccatagccga tcgggcatgc
      361 tcagatacac gtccacgact ctggggtcaa attgcgtgcc ggaacagcgc tggatttccc
      421 ttctggcaat ttcaatactt tgcgccctgc ggtacgggcg gtcggaggtg atggcatcga
      481 gcgtatccgc gaccgcaaaa atgcgtgacc ccagagggat ttcattgcct ttgagtcctc
      541 gtggataccc gctgccgtca tagtgctcct gatgggtata cacgatttcc gcagcttcgc
      601 gcagataagg aatcttggaa aggatctgat agccccgcac gcaatgttcg cgcatcaggg
      661 cctgctcgtc cggagtcagc ttaccaggtt ttaaaagaat ggcatctgga atggccattt
      721 ttcctatgtc atgcaggaag gcgccacgcg caataatacg aatctcagag ggagcaagcc
      781 ccatggcacg cgccagagca acggtatagg cggtcacacg tttggagtgt ccttctgttt
      841 ccgcatcctt taaatccagc gcgtccccca gagcctcaag tgtgacatca taggaacgct
      901 ccagatcggc aacagcgcgg cgcagcatct ctgttctggt tgcaataact tgctccaggc
      961 tggatggttg ctgggaactc tgcaggaggc ttcttctgta gctcaatgca tttcttaccg
     1021 ccatcagaag ttcgtccatt tcgacaggct tcagaaggca atcaaaagcg cctttgcgga
     1081 gggcgttgat ggcaacccgt gcgttggttg ggtcggtggc caggagtacg ggcgtgtctg
     1141 gttgcagctt ccggatgcct tccaacagat ccagaccatg gttttcgccc ataatgtcgg
     1201 aaagaacgag gtcgtaggga gggtccagcc gtaaatgttc gatggcttcc ggcccactgc
     1261 tggcagttac cgtatagtaa tgtgcatttt ccagagtaga ggcaatcatc gcccttcctc
     1321 tttgctctgt gtccaccacc agtattcgtt cggaagccat gaaatccttg ataagctaat
     1381 taatcattac aatgaattac aaaaaattac agcacagctc gtcctttttc agtagaaagg
     1441 caggcagaaa aacatctggc tgagtaactt tgggattaat tccgacttta atcgaaagat
     1501 ggtttccttt ccacgaaaat ttctcttttg tgtgaaaaat tctgaaaacc aagacttcac
     1561 caaaatggct ctggccgagc tatggttcgc gaatccattg ccttctgagt atgtgagcag
     1621 aagaccaaaa catacaatcg cttttatggc gtattttact gaaattctat ttgcgagcca
     1681 tcttattgtc tgattttcaa ccgtgacaac tgtaaatttt ctaatctgca tcttatttgt
     1741 aacattattt gtaacaatag ggataaaaat gctccatcct tcaggcagga aaggcgattc
     1801 gtgacaattg atttccgtgg gctttttcag cagacgaaac ctgatgttgt tgctgttcgc
     1861 gctgaagaac gccggaccca acatgtacag cgggacattt atgacagcca tcgccaccgg
     1921 atattctctg ttgcgtttta catgacaggg aatgaagtcc acgcggaaga gattctgacg
     1981 aacacctttg tgcaggcatt cacagttgcg cccaggccgg gccggcatgc gattgaccgg
     2041 gcattactct cccaactgcg gtcacggttt caaatttcac ctgtttcgac tgcggtcaca
     2101 gttcctgagt ctgggcagca cctggagaag aacatccttc gcacccatct ggaagaagca
     2161 ttgcagcatc ttccacctgt ggaaaggctg ctttttttat tgagagatgt agaggggtat
     2221 ccgctcgaag aagtttccag tctgaccgaa atcccacccg gagaggccag ccgcatcctc
     2281 atgtcagcca gaatccggct gcggagcatt ctggctgaca tgcaatcctg actaactgtt
     2341 cttcactact tcctttgccg ggcgatgcgt tcctttgtga gtcgtgaccc atcccggttt
     2401 agtggtctgg cgcgcacggc ccagcgcaag ggcctcttct ggaacatcct cggtaataca
     2461 tgatgcagcg gcaatatagg aaccgcgacc aatcacgatt ggggcaacca gggtagaatc
     2521 actgcctaca aacacgccat cttcaatgag agtgcgatgt ttattgatgc catcgtagtt
     2581 gcaggtaatg gtccccgccc cgatgttcac gccgctgccg atttctgcgt ctcccagata
     2641 gctgaggtga ttggccttcg agcctttgcc catacggatt ttcttcgtct cgacaaaatt
     2701 gccgatgtgt gcctcttcgc cgatttcgct gcccggacgc agatgtgcgt aaggaccaag
     2761 caatgcgccg gagcggatgt gcgattcact gataatgcag ccctgtcgca caaggacatg
     2821 gttctcaagc gtggagtttt caatgacgga ataggatcgg atgcggcagc cttcgccaat
     2881 acgggtatgt ccaagcaatt gcacgaaggg ttcgaggatg ctatcgggcg cgacctcaac
     2941 gccagcatca atcacagtcg tttccgggcg gaagatggtc acaccattgg ccatgagcct
     3001 gcgcgctgtc gccatgcgca tttcagcatc gagagcggcc agctctgcca gagtgtttgc
     3061 gccaagtact tcagttgcag acggggcttc cagcgcaacc acacgctgac cttcctcgac
     3121 cagaatgcgg gccatatccg tgaggtaata ttcgccgtgc gcgttatttg ttgtgagctc
     3181 gttaatatgg tcaaaaagtg gtttggtggc gaaagcgtaa atgcccgaat tgatctcgcg
     3241 gattttttcc tgttgtggtg tgagcgcctt ctgctcaaca atagcagcca cttctggcga
     3301 atgtgcagat ttgcgcaaaa cgcggccata gccgaagggg tcttccggct gcgcagtaag
     3361 gatggtcatc gcggcgtgct cggcgatgtg aaaatcacga agctgtgcaa tggtcttcgc
     3421 atcaagaagc gggacgtctc cagagagaac aaggatgtgt tcgtaacctt gtacagcttg
     3481 acgcgcgcat tggatggcat gtccggtgcc gcgctgttca gcttgtatga cgaaattcac
     3541 gccagtgtgc gccatttctg actgtaccgc gcccgcctga tgtccaacga caacgaagat
     3601 gtcatgtgaa ggaacaatct gctgtgcagc atgaattaca tgctccacaa gggcctttcc
     3661 tcctatctga tgcagaacct tggcgcgttt cgatttaagc cgcgtgcctt ttccggcggc
     3721 cataatgaca atggcgattc gcatgttact gctctccttc agggtttccg ggaccgcctt
     3781 gcccctgagg cggaagcccc ggaatggtga tgtcaatggg ttgcggggtg cccatgttca
     3841 ggctggtcag cggcgcgcca aactcctttt gatttcccac aaccagcacc gccaatttgg
     3901 ccggatcaat atatttctgt gcaacatgtt ctacgtcagc aaccgtcacc ttttccacac
     3961 tgtctcggta cttttcgagg aagtcagcgg gatatccata gaattcgagc ttcgctgctt
     4021 cagcaaggac cttctctttg gtgtcgtaat tgaagatgaa cgagttcagg acttcgtctt
     4081 tggcacgccg cagctcatct tcggtaaagg gtttggtctt cagatcacgg atttcttcca
     4141 gcatggcctc tgccgcctgg atggtcgttg ggctttttgt tcccgcaacg acgcggaaca
     4201 tccctggatg atcataggct gcgccatagc ctccgccaac ggcatatgca agacctcgct
     4261 ttgtgcggac gtcctgaaag aggcgtgaac caaagccgcc ggagaaaatt tcgttcatta
     4321 cagaaagcgc atagaaatcg gggttgctgc gttctgtacc cagccccacg atgtagatgt
     4381 tgctttggtt cacatcattt ttatctacgt aatagacgcc gggtttcggc ccggagaagt
     4441 cttccttaag cggagggact gcttcgccgc ctggcaggct gctgaaggca tcgcgcagcg
     4501 tcttctccat tgcgccggaa ttgaaatcgc cggagacgcc aatgatgatg ttattcccga
     4561 cgacagtacg gtcgtgccac ttcttcagat catcaagcgt gacggccttg atggtcgcca
     4621 actcgggctg ccgtgcatag gggctgtttg cgccatagac cagctttgtt gcctcgcgcg
     4681 cggcgatgcc ttcggcgtca tcattgcggc ggacaatcgc cgcagcggcc tgctgcctgg
     4741 caagctgcag cttgtcttcg cggaagaccg ggtgctcgag caggtcagtg acgatgttaa
     4801 agacgaaatc ttcgtctttg gtcaggcagg accaggagag ggaagtggaa tccgtatcgc
     4861 ctccggactc gaccttcgct gcgcgcgcct cgagcaggtc atcaagctgg tcgccgttat
     4921 gcgtggcggt gccgctggtg cgccaggtgt cggcgtacag gcttacgaga cctgctttgc
     4981 ctgggtcctc atcacgcgaa cccccgcgca tttcaataaa gccgttgacg aagggcaact
     5041 cgtggtcttc ctgaagaagg atgacgatgc cgttcggcag tttgatgcgc ttcggctgct
     5101 gcgggtggaa tggatgcagc ggtggaatgg ggacctgctt ccagggctgc gtctgctggg
     5161 aaaacgcagc ttgtgtgaaa gagaacagtg caactaaaac cgttaatcca agaagcagta
     5221 aaaaggcttc tttgaatggt cttttactgc gctggttctg agaatggaag ccgcgttgat
     5281 aacgaatcat tgcgcgcctc ccttcgtctg ctgttgtgga ggctgaaatt cgaccatagc
     5341 gtatgttctg ttggttggaa caaaaatttt gttggcgacg cggcggatgt ctgccttgct
     5401 gactgcatca atccgtttga gctg
//
LOCUS       DASRBV010000332         5795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_329348, whole genome shotgun sequence.
ACCESSION   DASRBV010000332 DASRBV010000000
VERSION     DASRBV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329348"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFA02_12375"
     CDS             complement(<1..1280)
                     /locus_tag="VFA02_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZU10690.1"
                     /translation="MPTDVIMPQMGESIFEGTITKWLKKPGDSVQRDEPLFEISTDKV
                     DAEIPSPAAGVLKEIKVAEGTTVQINTVVAVIDEAGNATASAPAPAPAASPAAEAKPE
                     APASSPAPAPAPSGNGQAKTDVIMPQMGESIFEGTITKWLKQVGDTVQRDEPLFEIST
                     DKVDAEIPSPAAGVLTEIKVPAGQTVQINTVVGVIGGEAGAAAPVSAPAAPPAKVEAP
                     AAAPAPVAAATAGVKEAPRSSPLVRKIAKDNNLDLSQVPGTGAGGRITKEDVLGFIEK
                     HGAAAPVPATPAPAPAPAAAKPAAPAAPAAPVTSGLPGELVPMSRMRAIIAQRMVDSK
                     RTNAHVHTVFKIDFTRIVKIREKEKSKYEQRNGVKLTYMPFISRAVIATLRKMPIINA
                     SMEGDAIRYHQNINLGIAVALDWGLIVPVIKQAE"
     gene            complement(1369..2154)
                     /gene="lipB"
                     /locus_tag="VFA02_12380"
     CDS             complement(1369..2154)
                     /gene="lipB"
                     /locus_tag="VFA02_12380"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZU10691.1"
                     /translation="MILSILDLGRLDYATGLALQKTLVELRHQQRIGNVLLLLEHPPV
                     LTLGRNSSRSNILASDELLAKRGVELHEINRGGDVTYHGPGQLVGYPIFDLRSFSPRL
                     GAVEYVRKLEEVLMRACGDYGIPAQRIAGRTGVWTLAGGSVQEKKMAAIGVHISRGIT
                     SHGFALNVTTDLRDFDLIVPCGIADREVTSMELETGATPGMESVMYTVARYFGQVFNA
                     QILWLESLEALLPSPADGPAQDTPVSIPKELKQLRGVDPTHFA"
     gene            complement(2352..2567)
                     /locus_tag="VFA02_12385"
     CDS             complement(2352..2567)
                     /locus_tag="VFA02_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZU10692.1"
                     /translation="MQNEFTAVIERDGEWFVAWSPEVPGANGQGKTLQECKENLAEAI
                     HLILEDRRQDGLRGLPENAICEVITVQ"
     gene            complement(2678..4099)
                     /gene="lpdA"
                     /locus_tag="VFA02_12390"
     CDS             complement(2678..4099)
                     /gene="lpdA"
                     /locus_tag="VFA02_12390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZU10693.1"
                     /translation="MAETTYDIAIIGGGPAGYTCAIRAGQFGLKTALVEASDKLGGTC
                     LHVGCIPTKALLFDAEIYDHLKHGKDYGIEIQGEAKINWKTVLERKNQIITTHTKGLD
                     YLMRKNKVTVINGYGKLTGPAKNGVHAIEVKDAAGKTSTIQTRNVVLATGSDAKMLPG
                     LTADDKILTNIEILSIPQVPKSLIVIGAGAVGVEFASIFRSFGSEITLVEYLPRLVPA
                     EDEEVSKELARAFSKRGIAAHTGAKVEKIEKTKTGVKVTFTASDGKQVVKEAEKVLVA
                     VGRAPRTENIGLEKTKIAPERGFIKVNEWMQTDEPGVYAIGDIVAGLPQLAHVGSMAG
                     MVVAAKVADKYARPVKRQRIPACTYTEPQIGSVGLTEAQAKEKGLNVKVGKFPFKGNS
                     KATIVNSHDGFIKVVSDAKYGEVLGVHIIGPQATELIAEAVTAIELEATVEDMMYIIH
                     AHPTLSEAMLDAFGSVEGKAINV"
     gene            4259..4789
                     /locus_tag="VFA02_12395"
     CDS             4259..4789
                     /locus_tag="VFA02_12395"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZU10694.1"
                     /translation="MSAIPHPIKVSVGEYLATSYRPDCDYVDGEVQERNLGEKEHSLL
                     QRALVFLFMKNSGTWQVEVYPELRVQVSPTRFRIPDITVTSAGLQWERILKAPALLFI
                     EILSPEDTMRRIRERVDDYLKFGTKNVWVIDPDLRKAYVCSEHGFMEPEDGVLAVPET
                     KIRVVLNELFAELDRG"
     gene            complement(4786..5775)
                     /gene="hemB"
                     /locus_tag="VFA02_12400"
     CDS             complement(4786..5775)
                     /gene="hemB"
                     /locus_tag="VFA02_12400"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZU10695.1"
                     /translation="MEYPVTRLRRLRTSEPMRSLVRETHLEPGALIYPLFICPGEGVR
                     NEISSMPGVFNLSIDEALKEAESVASLGIGGLLLFGLPENKDEQGSGAWSEDGIVQRA
                     LREFKSSTALKTLLMIADVCLCEYTSHGHCGVVRKQGESYEIENDPSVQLIARTAVSL
                     AEAGADIVAPSDMMDGRVAAIRDGLDEAGLENTPILSYASKFASAFYGPFREAADSAP
                     QFGDRRSYQMDGANLREAMREIELDLAEGADMVLMKPAMPYMDVIRAARERFDVPIGA
                     YQVSGEYSMLHAAFAKGWLEPQRAMMESLISIRRAGAGFIVTYFAKEAAKVLG"
BASE COUNT         1184 a         1757 c         1558 g         1296 t
ORIGIN      
        1 tcctcggcct gcttgatgac cgggacaatc aggccccaat ccagcgcgac cgcaatgccc
       61 agattgatgt tctggtgata gcgaatcgca tcgccttcca tcgaagcatt gataatcggc
      121 atcttgcgca gcgtggcaat cacagcccgc gagatgaacg gcatgtaggt tagtttcacg
      181 ccattcctct gctcgtactt tgacttctcc ttctcgcgga tcttgacaat gcgcgtgaag
      241 tcaattttga agactgtatg cacatgagca ttggtccgct tcgagtccac catgcgctgc
      301 gcgatgatgg ccctcatgcg gctcatggga accagctcgc ccggaagccc ggacgtgact
      361 ggggccgcag gagcagccgg cgctgccggt tttgccgcag ctggagcagg tgctggagcc
      421 ggggtggccg gaacaggagc agcggcaccg tgcttctcaa taaagccgag cacgtcttct
      481 ttggtgatgc gtccgcctgc gcctgttccc ggaacctggc tcaggtcaag gttgttgtcc
      541 tttgcaatct tgcggaccag cggcgaggag cgcggcgctt ccttcacgcc ggcagtagcc
      601 gcagcaactg gggcaggcgc agccgccggg gcctcaactt tcgcaggcgg cgcggcaggt
      661 gctgatacag gtgccgccgc cccggcttct ccgccaatca cgccgaccac ggtgttgatc
      721 tgcactgtct gccccgcagg caccttgatt tccgtcagca ctccggcagc aggcgatgga
      781 atctcggcat ccaccttatc ggtcgaaatc tcaaagagag gctcatcgcg ctgcactgta
      841 tcgccaacct gcttgagcca cttcgtaatg gtgccctcaa agatggattc gcccatctgg
      901 ggcatgatga cgtcggtctt tgcctgtcca ttgccggaag gcgcaggcgc cggtgcagga
      961 gagctggcag gcgcttccgg tttcgcctct gccgccggag aggccgccgg tgcaggtgca
     1021 ggtgccgatg ctgtcgcgtt ccccgcttcg tcaatcacag cgacaacggt attgatctgg
     1081 acagtcgtgc cctcggccac tttgatttcc ttcagcactc cggccgcagg cgatggaatc
     1141 tcagcgtcca ccttatcggt cgaaatctca aaaagaggct cgtcgcgctg gacgctgtct
     1201 cccggcttct tcagccattt ggtgatggtc ccttcaaaga tagattcgcc catctggggc
     1261 atgatgacat ctgtcggcat cggttccctt tccctgtttc aagcgcaaat atggattata
     1321 aattcggcaa tctggccgca aagtctggtg ctgaccaaac cttttagttt acgcgaaatg
     1381 cgttgggtcc acacctcgga gttgcttcag ttcttttgga atagacacag gagtgtcctg
     1441 cgcagggcca tctgcgggtg acggcaacag ggcttccagc gattccagcc agaggatctg
     1501 cgcattgaag acctgcccaa aatatcgggc aaccgtgtac atcacgctct ccataccggg
     1561 agttgcgcca gtttccagct ccatgcttgt cacctcgcgg tcggcaatcc cgcatggaac
     1621 gatgaggtca aagtcgcgca ggtcggtggt cacgttcagc gcgaatccat gcgatgtgat
     1681 gccgcgcgaa atatgcacgc caatcgcggc catcttcttt tcctggacac tgccgcccgc
     1741 cagcgtccag acacctgtac gcccggcaat gcgctgcgcc ggaattccat agtcgccgca
     1801 ggcgcgcatc agaacttctt ccagcttccg tacatattcc actgcgccca ggcgcggcga
     1861 aaaactgcgc aaatcaaaga tgggatagcc cacaagctgg cccggaccgt gataggtcac
     1921 atcgccgcca cggttaattt cgtgtagctc cactccgcgc ttcgcaagca gctcatcgct
     1981 ggcaagaata ttcgagcggc tggaattgcg tcccaacgtc agcaccggag gatgctccag
     2041 cagaagaaga acattgccaa tccgctgctg atggcgtaac tccaccagcg tcttttgcag
     2101 ggccagcccg gttgcgtagt caagacgccc gagatcaagg atggagagaa tcatgttcta
     2161 tggtttgcgc ccgatttcag gaacggaaag accacggcat atcttcctgg caagcagatc
     2221 ggcaatttct gtatgccggg gcacagcttc gattctgccg gtttgcggat tgcaccaaat
     2281 agaatgcgat ccgccttccc gtttcaagta acagccatac aagcgcagat gacggagaag
     2341 agtgtcgcgt ttcactgtac cgtaatgact tcgcaaatgg cattttctgg taacccacgc
     2401 agaccatcct ggcggcggtc ttccagaatc agatgaatgg cctcagccaa attctctttg
     2461 cattcctgta aggtctttcc ctgaccattt gcacccggaa cttccggcga ccaggcaaca
     2521 aaccattctc catcgcgctc aatgaccgca gtaaactcat tctgcatact ttcagtatag
     2581 tccccgtaag aagaccgttc gcggctgggg actggagact aacaaggaac aagtagtccc
     2641 ctgtttttcc ccggccacca ctgaaacgaa aactgctcta cacattgatc gccttgcctt
     2701 ccacgcttcc aaacgcatcc agcatcgctt cagacagcgt cgggtgcgcg tggataatgt
     2761 acatcatgtc ctccaccgtt gcctcaagct cgatggcagt cactgcctcg gcaatcagct
     2821 cggttgcctg cgggccgatg atgtgtacac ccagtacctc gccatacttt gcatcgctga
     2881 ctaccttgat gaagccatca tgcgagttga cgatggttgc cttcgagttg cccttaaagg
     2941 ggaacttgcc caccttcaca ttcaggccct tttctttggc ctgtgcttcc gtcagaccaa
     3001 cgctgccaat ctgcggctcg gtataagtgc aggccgggat gcgctggcgc ttcaccggcc
     3061 gcgcatactt gtccgcaacc ttcgcggcga ccaccattcc cgccatcgag cctacatgcg
     3121 caagctgggg cagtccggca acaatgtcgc cgatcgcgta aacgcccggt tcatccgtct
     3181 gcatccactc gttgaccttg atgaaacccc tctccggagc aatcttcgtc ttctccaggc
     3241 caatgttctc agtccgcgga gcgcgcccca ccgccaccag caccttttct gcctccttca
     3301 ccacctgctt gccatcggat gcagtaaagg tgacctttac gccggtcttt gtcttctcga
     3361 tcttctcaac cttcgccccg gtatgcgctg ctatgccgcg tttgctgaac gcgcgcgcca
     3421 gctccttgct gacctcttca tcttctgcgg gaacgagccg cggcaggtat tccaccagcg
     3481 tgatttcaga accaaagctg cggaaaatag aagcgaactc aacgccgaca gcacccgctc
     3541 cgataacaat cagcgatttc gggacctgcg gaatcgagag gatctcgata ttggtcagga
     3601 ttttatcgtc cgccgtcagc ccgggcagca tcttcgcgtc agaacctgtg gccagcacca
     3661 cgttcctggt ttggatggtc gaggtcttcc ccgccgcgtc cttgacttca atcgcatgga
     3721 cgccgttttt cgccgggccg gtcagcttgc catagccgtt gatgaccgtg accttgttct
     3781 tccgcatcag atagtccaga cctttggtgt gtgtggtgat gatctggttt ttgcgctcga
     3841 gtacggtctt ccagttgatt ttggcctcgc cctggatttc aatgccgtag tctttgccat
     3901 gcttcaggtg gtcgtaaatc tcggcgtcga agagcagcgc cttggttggg atgcagccta
     3961 catgcaggca ggtaccgccg agcttgtctg atgcttcgac cagggcggtc ttcagtccaa
     4021 actgtccggc gcggatggcg cacgtgtatc cagccggtcc tccgccgata attgcaatgt
     4081 catacgttgt ttctgccaag gaatgctccg ttcaaaattt aggatcagat gtgccgggcg
     4141 caaaaagaac gccgaactat tgattctatg cgagaagaaa cgattcacgc gagtcgctgg
     4201 gcagttcccc gtcgccacaa gaccaaatct gcgcgatact tacaggagag ggcagcttat
     4261 gagcgcaatc ccgcacccca tcaaggtctc cgtgggcgaa tacctggcta ccagctatcg
     4321 cccggattgc gactatgtgg acggcgaggt gcaggagagg aatttgggcg agaaagagca
     4381 ctctttactt cagcgggcgc tggttttcct ttttatgaag aacagtggga catggcaggt
     4441 cgaagtctat cccgaactgc gagtgcaggt cagcccgacc cggtttcgta ttccggatat
     4501 aaccgttaca tctgctggac tccagtggga acgcattctt aaggctccag cgctgctctt
     4561 catcgaaatt ctttcaccag aagacaccat gcgccgtatc cgcgagcgcg tggatgacta
     4621 tctgaaattt ggcacaaaaa atgtctgggt catagatccg gatctgcgca aggcatatgt
     4681 ctgttcagag cacggcttca tggagccgga agatggtgtg ctggccgttc ccgaaacgaa
     4741 aattcgtgtt gtgctgaatg agttatttgc ggaactggac cggggttagc cgagcacctt
     4801 cgccgcttct tttgcaaagt acgtcacgat aaaacccgcc ccggcacggc gaatcgaaat
     4861 cagcgactcc atcatcgccc gctgtggttc gagccagcct ttggcgaagg cggcgtgcag
     4921 catcgagtat tcccctgaaa cctgataagc gccgatcggt acatcgaagc gttcacgcgc
     4981 ggcgcggata acatccatat aaggcatcgc aggtttcatc agcaccatgt ctgcgccctc
     5041 ggccaggtcc agttcaattt cccgcatcgc ctcgcgcaaa ttcgcgccat ccatctggta
     5101 actgcggcgg tcgccgaact gcggtgctga atctgccgcc tcgcggaacg gcccatagaa
     5161 ggccgaggcg aacttggagg cgtatgaaag aatcggagtg ttttcgagtc cagcttcgtc
     5221 aagcccgtcg cggatggcag ccactcttcc gtccatcatg tcgctcggcg caacgatgtc
     5281 ggccccggcc tcagccagcg acacggccgt ccgcgcaatc agctgcacgc tcgggtcgtt
     5341 ttctatttca taggattcac cctgctttct caccacgccg caatgcccgt gcgaggtgta
     5401 ctcgcatagg caaacatcgg caatcatcag cagggtttta agcgccgtgc tgcttttgaa
     5461 ctcccgcagc gcgcgctgca caatgccatc ctcgctccat gcgccggaac cttgctcgtc
     5521 cttgttctcc ggcaaaccaa acaaaagcag cccgccaatg ccgagcgacg cgacgctttc
     5581 agcctctttc agcgcctcgt ctatggaaag attaaagacc ccaggcatgg aactgatctc
     5641 attgcgcacg ccttcacccg gacagataaa gagcggataa atcaatgcgc cgggttccag
     5701 atgggtctcg cgcacaagcg agcgcatcgg ctcgctcgtc cgcagacggc gcaggcgggt
     5761 cacagggtat tccatggatt tattttaacg aggca
//
LOCUS       DASRBV010000333         5602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_538626, whole genome shotgun sequence.
ACCESSION   DASRBV010000333 DASRBV010000000
VERSION     DASRBV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538626"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFA02_12405"
     CDS             <1..181
                     /locus_tag="VFA02_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU10696.1"
                     /translation="SLTTVRQPLREMGQQGAQILLDMIAHPENNFPEEVVMQPELIVR
                     ESTGPAAAQKSKRKK"
     gene            complement(332..3844)
                     /locus_tag="VFA02_12410"
     CDS             complement(332..3844)
                     /locus_tag="VFA02_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10697.1"
                     /translation="MQAQEYRGTISGQVTDPKGAVIPNAGITAVGPQQTYSITSGANG
                     NYVIPYVQPGTYTVTAEAPGFKKAVQQNVVIDVATKASVNFSLQIGSTSETVSVEENQ
                     LGLNLADASVGAVMDPEKVQNLPLNGRQIYMLMALTPGVRFNQTQFGATGYSGTRGWD
                     ESNNYSITGIPGAYNQFYLNGAPISQQGGGGAGTWNISPSIDAVQEFKIMTITYDAQY
                     GRVGGGAMNTILKSGGPHFHGTLYDFWRNSILDANTYQLNQQGARKPFHNMHQFGGTI
                     GGPFLKNNKGFFFFSYEGWREVLPGGIVTTVPTPDMFPDANGNVNLTNYLAAVKKNGI
                     YDPLTTTACTSGVNGCPKSGYMRQQFPNNTIPASRISPIGLNILKLFPAPNRAGYVNN
                     YVFNGSDQYRYNMPIARVDYNFTDKTRLYGIFAWWSGHEYRNTNGMSGPAIRGNINNY
                     RSSITQVLDLTHTFSPDLVGDIRLSFNRMWNLAPDGTLRAGLASLTPDQLGLSMPQIP
                     TTKHRYAPEININDGYPGIIGNTGDPVMYETYDGAPSLTQVFHQHTFHYGGEVMVYHD
                     VTGGVGQPNGNFSFGTGFTQRDPFQGNQDGASIASVLMGYPSGGSVQYALPPYESYHY
                     YAAFLQDDWKARSNLTFNLGLRWDTETSPVERHHHLLAGMCLTCINPITNMITFPPNN
                     TLPNGAKMVNPIVGGVQFSSDKLTAYENTWGIFQPKAGFSYLLNKDILLRGGYTLSKA
                     LGIELGGASAWNQSTSYNSSPDSGLHPADDFRNGNPFPFGAAPPPGNSNGLMTLVGTG
                     FGIDLRNRKIPLVHEWALGVELQAPWQILLDLTYLGNHATDMRASKQFNGLNPADFYH
                     GHDDPNYLNQLVTNPFYGVLPPTIDMGRNPTIQAKLLMVPYPQYNGNLYIYTDPKGFS
                     NYHAMLVKAEKRFSTNGGALSKGLSFLTSFTWSKLMVANGYLNNGGAGLVDAKPYYVV
                     DGTDRPWDLAFSGLYGLPIGRGGAIASGAHGLIGQIINDWQLDWIFTNAGGTPVGYPN
                     GDIYHCNGKYNIISPHKTWNSYINNTEPSCWNTFPPYTAVTALPRTTVVRNPWAQQTQ
                     IGMEKRFLLHEGTYLQFKAEAFNATNTPIFGGPNTGGPENPPSRNTSVANPNQPGAWS
                     GYGTIGSTQQNFPRQIQLSLKLLF"
     gene            complement(4277..5488)
                     /locus_tag="VFA02_12415"
     CDS             complement(4277..5488)
                     /locus_tag="VFA02_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZU10698.1"
                     /translation="MQKKTLTCAIVALASLTAFAQTPGNWVGTWAASPLPHAVNPVQV
                     APTNSTYRNVVHISIGGNAVRVQLTNEFGTEPLVVGAAHVAAGAGGGSEQQGTDHALT
                     FNGKPTAMIPAGAFILSDPVPMQVAPLSDLIVSVYLPEQHIRNMTCHSFADETNYILK
                     GDATAAPTPDNSSPINSWCFVKGVDVSTNATDHAAAIVAFGDSITDGAASTPNANHRW
                     PDVLAARLQADKRTAHLGVLNEGIGGNRVLHDGYGPSALARFDRDVIAQSGVKYLIVL
                     EGINDIGHLKRPEGPEDKPITADDLIFGLSQLVTRAHQHGIKVFGATLTPYVGAGYST
                     AEGEQIRQAYNQWIRTSGVFDGVIDFDKATQDPAHPDTFLPEYDSGDHLHPKDAGYKA
                     MGDAIDLSLFR"
BASE COUNT         1292 a         1558 c         1473 g         1279 t
ORIGIN      
        1 cagccttaca acggtccgcc agcctctccg tgaaatgggc cagcaaggcg cacagatcct
       61 tctcgacatg atcgcgcatc ctgaaaacaa cttccctgaa gaagtcgtca tgcagccgga
      121 actgattgtg cgcgaatcca cgggccccgc cgcggcgcag aaatccaaac ggaagaagta
      181 aaaagacagg gcgccggaat ttttcccaac gccctgggag gctaaagcat tttcatagac
      241 actctggacc atgccacgaa acttcagtcg ccgcgaccaa cagcgggccg gcggcactgc
      301 acaacgcaaa tggagaatct gtgaaattgc cttaaaagag caatttcagc gatagttgaa
      361 tctggcgcgg aaagttctgc tgcgttgagc cgatcgtccc atatcctgac cacgctccag
      421 gctggttcgg attcgccacg ctggtattgc gtgatggcgg attttccgga ccacccgtgt
      481 ttggccctcc aaagataggt gtattggttg cgttgaaagc ctcggccttg aattgcaggt
      541 aagtgccttc atgcagaagg aaccgcttct ccattccaat ctgtgtctgc tgcgcccatg
      601 gattgcgcac aaccgttgtc cgcggaagcg cagtcacagc cgtataaggt ggaaaggtgt
      661 tccagcacga aggctctgtg ttattgatgt agctgttcca ggtcttgtgc ggtgagatga
      721 tgttgtactt cccgttgcag tggtagatgt ctccattggg atacccgacc ggtgtcccgc
      781 ctgcattggt aaagatccag tccagttgcc agtcgttgat gatctggccg ataagtccat
      841 gagcgccgct tgcaatggcg ccgccacgtc caatcggcag accatagagg ccgctgaacg
      901 ccaggtccca cggacggtcg gtgccatcca ctacgtaata cggcttcgcg tctacaagcc
      961 ctgccccgcc attgttcaga tagccattgg ccaccatcag cttcgaccat gtaaatgacg
     1021 tcagaaagct gagtccttta cttaaggccc cgccgttggt agagaaccgc ttctcggcct
     1081 ttaccagcat ggcgtggtaa ttgctgaatc ccttcgggtc ggtatagatg tagaggtttc
     1141 cgttgtattg cggatacggc accatcagca gctttgcctg gatcgtcgga tttctcccca
     1201 tgtctatggt cggcggaagc actccataga acggattggt cacaagctgg ttcagatagt
     1261 tcggatcgtc gtgaccgtga taaaaatcag ccggattcag gccattgaac tgcttcgatg
     1321 cgcgcatgtc tgtcgcatga tttcccagat aagtaaggtc gagcaaaatt tgccagggtg
     1381 cctgcaattc aaccccaagc gcccattcat gaacaagcgg aatcttgcgg ttacgcagat
     1441 caattccaaa gcctgttcca acaagcgtca tcagtccatt ggagtttccc ggcggcggag
     1501 ctgctccaaa aggaaacggg tttccattgc gaaaatcatc cgcaggatgc aggccgctgt
     1561 ccggcgacga gttgtaactg gtgctctggt tccatgctga tgcgcctcca agctcaatcc
     1621 ccagcgcctt actgagcgta tagccgcccc gcagcagaat gtccttgttc agcagataag
     1681 agaatcctgc tttgggctga aaaattcccc atgtattttc atatgcggtg agcttgtcgc
     1741 ttgaaaactg cacgccgcct acgattggat tgaccatctt cgcgccattc ggcagcgtgt
     1801 tgttcggcgg aaacgtgatc atgttggtaa tgggattgat gcaggtcagg cacatgcccg
     1861 ccagcagatg atggtgccgc tccaccggtg aagtttccgt gtcccaccgc agcccgaggt
     1921 taaacgtcag attgctgcgc gccttccagt cgtcctggag aaaagcagcg taatagtggt
     1981 agctctcata aggaggcaga gcatactgca cgctgccgcc gctgggatat cccatcagca
     2041 cactcgcaat cgaggcgccg tcctggttcc cctggaacgg gtcgcgctgc gtgaatccgg
     2101 tgccgaatga gaagttgccg ttcggctggc ctacacctcc ggtcacatca tggtagacca
     2161 tcacttctcc gccataatgg aacgtgtgct gatggaagac ctgcgtcagc gatggcgctc
     2221 catcataggt ctcatacatg accgggtcgc cagtgttacc gataatcccc ggatacccat
     2281 cgttgatgtt gatctccggc gcatagcgat gcttcgttgt aggaatctgc ggcatagaca
     2341 atccaagctg gtccggcgtc aaactggcca gccccgcgcg gagcgtcccg tccggtgcca
     2401 ggttccacat acgattaaag gagaggcgga tgtcgcccac aaggtcagga ctgaaggtgt
     2461 gcgtcaggtc aagcacctgc gtaatgctgg agcggtagtt attgatgttg ccccggatcg
     2521 ccggtccgct catgccattc gtattgcggt attcatgacc tgaccaccag gcaaatatcc
     2581 cgtataaacg tgtcttgtcc gtgaagttat agtccacacg cgcaatcggc atgttgtagc
     2641 ggtactggtc tgatccgtta aagacataat tgttgacgta tccggcgcgg ttcggggccg
     2701 ggaaaagctt gagaatgttc aggccaatcg ggctgatgcg gctggcggga atggtgttgt
     2761 tcgggaactg ctggcgcata tagcctgact ttggacagcc atttacgccg gaggtacagg
     2821 ccgtagtcgt caacgggtca taaataccgt ttttcttcac cgcagccaga taattcgtca
     2881 ggttcacatt gccgtttgcg tccggaaaca tatcgggagt aggaacagtg gtgacaatgc
     2941 cgccgggcag cacttcgcgc catccttcat agctgaaaaa gaagaagcct ttgttgtttt
     3001 tcaggaacgg tccgccaatg gtcccgccaa actgatgcat gttgtggaag ggctttctcg
     3061 cgccctgctg gttcagctga tacgtgttcg catccagaat cgaattgcgc cagaaatcat
     3121 acagagttcc gtgaaaatgc gggccgccgc ttttcagaat cgtattcatg gcgccgccgc
     3181 ccacgcgccc atactgcgca tcgtaggtga tcgtcatgat tttgaattcc tgcacggcgt
     3241 ctatggaagg cgaaatgttc catgtacctg cgccgccccc gccctgctgc gaaatgggcg
     3301 cgccattcag ataaaactgg ttgtaggccc cgggaattcc ggtaatcgaa tagttgttcg
     3361 attcgtccca tccgcgcgtg ccggaatacc ccgtggcccc aaactgtgtc tggttaaacc
     3421 tcacgcccgg tgtgagcgcc atcagcatgt aaatctggcg gccattcagc ggaaggttct
     3481 gcaccttttc cgggtccatc accgcgccga ctgaagcatc agccagattc agaccgagct
     3541 ggttctcctc gaccgaaacc gtttcggaag tcgaaccaat ctgtaatgag aaattcacgc
     3601 tggcctttgt ggccacatca atcaccacat tctgctggac tgctttctta aatcccgggg
     3661 cttcagcggt caccgtatat gttcccggct ggacatacgg aatcacataa ttcccattcg
     3721 cgccgcttgt aatcgaatat gtctgctgtg gaccaaccgc cgtaatgcct gcattcggta
     3781 tcactgcgcc ctttggatca gtcacctggc ctgagatcgt gccgcggtat tcctgcgcct
     3841 gcaccagcga agttgtcaga aaaaatatca ggcacagcag cggtaaatgg catctgttta
     3901 aacgcttaaa tggaatcgtt tccaacctcg aacgaaagag catattacgt ttcattttgt
     3961 ctggcctcaa aatgagtggg ttttacagcg acaaggataa taacaacaaa attggaatcg
     4021 tttccaatga aaattttatt tatttgtaaa taaaagagtt agaaagggtg gtaaccaaag
     4081 tactgcgcgg gaaaccagcc gggacgggcc cacacatgca tgggagatgt tatgaccagg
     4141 aatttttcct gagtcatctc acaaaccagc acagaatccc accggcccat attctgaaag
     4201 cccgcaaagc agacgctgac tgctgaccgc ttttagctgc ttgtgtgaga tgacctcagg
     4261 gcctgaaaac aggcacttac cggaacagcg acaaatcaat cgcatcgccc atggctttat
     4321 aaccagcatc tttcggatgc agatggtcgc cggaatcata ctcaggcaaa aaagtgtccg
     4381 gatgcgcagg gtcctgcgtg gctttgtcaa aatcaatcac accatcaaac acaccgctcg
     4441 tcctgatcca ttggttataa gcctgccgaa tctgctcccc ttccgcagtg gaatagccgg
     4501 ctcccacata aggcgtcagc gttgcgccaa ataccttgat gccgtgctga tgtgctcgtg
     4561 tgacaagctg tgaaagccca aagatcagat cgtctgcagt aataggcttg tcttccgggc
     4621 cttcaggacg cttcaggtgt ccgatgtcat tgattccctc gagaacaatg agatacttca
     4681 ctccgctctg cgcaatcaca tcgcggtcaa agcgggccag ggcgctggga ccatatccat
     4741 cgtgcagaac acgattgccg ccgatccctt cgttcagtac cccaagatgc gccgtccgct
     4801 tgtccgcctg gagccgagca gccagaacat caggccagcg atgattggcg tttggagttg
     4861 acgccgcacc atcagtaatg gagtcgccaa acgcaacaat cgccgcagcg tggtctgtgg
     4921 cattcgtgct cacgtccact ccctttacaa agcaccagga attgataggg ctggaattgt
     4981 ccggagttgg agcagccgta gcatctccct tcagaatgta attcgtctca tccgcgaacg
     5041 agtggcaggt catgttccga atgtgctgct caggcagata cacactcacg atcaggtcag
     5101 acaacggcgc tacctgcatc ggcacaggat cgctcaaaat aaatgcgccg gcaggaatca
     5161 tggccgtagg cttgccattg aaagtaagcg catggtccgt tccctgctgc tcggaaccgc
     5221 cgcctgcacc cgcagcaaca tgcgccgcgc ccactaccag cggctccgtg ccgaattcat
     5281 tggtcaattg aacacgaaca gcattcccac cgatgctgat atggacaaca tttcgatacg
     5341 ttgaattcgt cggtgccacc tgcaccggat ttacggcatg gggcaacgga gaagcggccc
     5401 atgtcccgac ccagtttcca ggagtttgtg caaaagcggt gagactggct agagcgacga
     5461 tggcacacgt gagcgttttc ttctgcattc tgttcccttc tcaagatgaa aatcagagca
     5521 aagccagcat aatgcagaga catgccttcg tgcacaaaca aacccttcca ccccaaatga
     5581 ttgttgtctg gccccacgcc gg
//
LOCUS       DASRBV010000334         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_23218,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000334 DASRBV010000000
VERSION     DASRBV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23218"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="VFA02_12420"
     CDS             complement(<1..1860)
                     /locus_tag="VFA02_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZU10699.1"
                     /translation="MKHNAAKLAIVSVGFLLFLGTASLHAQSASHWFKQGQNAEAKDD
                     IETAYEDYYKAFQKKPTDLRYKTAWERTRFAAAAEHVKRGQKLRDQGNDTAAVTEFLH
                     AIEIDPSNELAQQEIQATREKMNAPPTQETSLSPNATPALEEIGGPPTLKPISNEPIT
                     IHSVEDSRVIYQTVGKLAGINVLFDPQYTSKRIQVDLTNVSLYDALRIIGTISGTFWR
                     PITDNTIFVAENNRGKRTELDEQAVQTFYLANITQQQDLNDINTALRQVFTTARIYPV
                     PSQNAIIMRGTPDELLLAQKIINDLDKAKPEVVIDVAVMEVNRDKMRNIGIQLPQTAS
                     INFQQSNANQSSSSSSSSSSSTTSSTTTSTTGSLTLNDLAHLNATNFAVTIGQAQVNL
                     LLSDSDTKLLQNPRVRASDGQQAVLKIGEKLPVATGSYQTGAATAIVSSLVNTQFQYL
                     DVGVNVDMKPTVHYDRDVTIKMKIEVSAENGTVNLGGINEPVITQRSVDQTIRLREGE
                     ASILGGILMRQENKSLSGTPGLAELPILRYIFGSESREVRDDEIVFLVIPHVVRAERI
                     SPLNTEIVDSGTMNSIELRRIQAPAPAAKPAPVPAANQEQGTVPGQTPSVAAQN"
     gene            1969..2496
                     /gene="smpB"
                     /locus_tag="VFA02_12425"
     CDS             1969..2496
                     /gene="smpB"
                     /locus_tag="VFA02_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZU10700.1"
                     /translation="MARQSSHQVAPAASQKSKPRDPVAAGTRDAAQNRAAGHNYFLLD
                     RFEAGVALRGTEVKSIREGKANLKDAYGIIKDGEAFLLNAHIGPYSHTGYTTHDALRT
                     RKLLLHQEEIRKLQGKTQQKGFTLIPTRLYFRNGRIKCELALAKGKQDWDKRETERRR
                     EADREARAAIARSRK"
     gene            complement(2521..4374)
                     /gene="dnaX"
                     /locus_tag="VFA02_12430"
     CDS             complement(2521..4374)
                     /gene="dnaX"
                     /locus_tag="VFA02_12430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZU10701.1"
                     /translation="MAYQVLARKYRPQRFSDVVGQEHVTRTLLNALEQGRIAHGYIFS
                     GHRGIGKTTIARILAMALNCRTAIGSPERPTAEPCGVCDSCTEIRAGNAVDVIEIDAA
                     TNRGIDEIRELRDAARYRPARDKYKIYILDEAHQITDAAFNALLKTLEEPPGHVVFMM
                     ATTQPEDFPQTIRSRCQHFSFHAVKFDDILGQLRAIAGHENVAAEDDALALLAEAGDG
                     SMRDALSIMDQAIASAPVVDGRARLDAAQVRKLMGTVPNTVFERLLEAVGENNTSAVI
                     EEINTLLNAGNSPQALARQFVRYLRNVLMTRIAGENSELLQISADERARAARSAVLFS
                     EEDLTRFLQIMLRTYDELNYRQEQRFHLELGLIKLVHLQRLLPIEEFLSQMPSGGKPA
                     LGGGVLPKAGAASARSSQPVGPPPKPAFSPFEADRERKMTSEISASPKLEAPTALRAL
                     TPVEITSQAVKAPVTGVAEAAIEATDGSLALAPVASSHAEAEIDRIRESICAALEEKG
                     HNAAAVFLDEGRWTVQGDTIQVEVARTKTMIGLTMTPETEKIAKSAMRAVGATQKLAV
                     IPGESGTANGNAKPRAAAGGSVHAQAIEHPLVKRTMELFNAEVRSVLDLRK"
     gene            4510..>4731
                     /locus_tag="VFA02_12435"
     CDS             4510..>4731
                     /locus_tag="VFA02_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HZU10702.1"
                     /translation="MPSEHDAGRLFEEAVNIMARLRAPGGCPWDREQTFDSIRKYTLE
                     ETYEVLDTIERRDWSHLKEELGDLLLQVLF"
BASE COUNT          987 a         1372 c         1326 g         1046 t
ORIGIN      
        1 attttgcgca gcaacagacg gcgtctgccc gggcaccgtg ccctgttcct gattggcggc
       61 gggaaccggc gctggttttg cagccggagc tggcgcctga atccgccgca gctcaatcga
      121 attcatggtg ccggagtcca ctatttctgt attcaacggg ctgattcgct cggcgcgcac
      181 cacatgcgga atcaccagaa agacaatttc atcatcacgc acttcgcgcg attccgagcc
      241 aaaaatgtag cgcagaatgg gcaactcggc cagcccgggc gtaccgctca aggatttgtt
      301 ttcctgccgc atgagaatgc cgccgagaat gctggcttcg ccttcccgca gacggatggt
      361 ctggtccact gagcgctgcg taatgactgg ttcgttgatg ccgcccagat tcacagtgcc
      421 gttttccgcc gagacttcga tcttcatctt gatcgtgaca tcgcggtcgt aatgcaccgt
      481 gggcttcatg tccacgttca cgcccacgtc aagatactgg aactgcgtat tcaccagcga
      541 gctgacaata gcggtcgctg ctccggtctg ataagacccc gtggccacgg gcaacttttc
      601 gccgatcttc agcaccgcct gctgcccgtc cgaggcccga acccgtgggt tctgcaagag
      661 ttttgtatcg gaatcgctca acagaaggtt gacctgcgcc tgcccaatgg tcaccgcaaa
      721 gtttgtcgca ttcaggtgcg ccaagtcatt cagcgtaaga ctgcctgtag tgcttgtggt
      781 tgtggaagat gtagtggacg acgacgagga tgaagatgaa gacgagctct ggttcgcgtt
      841 cgactgctga aagttaatcg aggcagtttg cggaagctga atgccgatgt tgcgcatctt
      901 gtcgcgattg acttccatca cggcaacatc aatcacaact tcaggcttgg ccttgtccaa
      961 atcattgatg atcttctgcg ccagcagaag ctcatccgga gtaccgcgca taataatcgc
     1021 attctggctg ggcacgggat agatgcgcgc ggtggtgaac acctgccgga gcgctgtatt
     1081 gatatcattc aggtcctgct gctgggtaat gtttgccagg taaaaggtct ggactgcctg
     1141 ctcatccagt tccgtgcgct tgccgcggtt gttctccgca acaaagatgg tgttatctgt
     1201 tatcgggcgc cagaatgtgc cggagatcgt tccaataatg cgcagcgcat catacaaaga
     1261 gacattcgtc aggtctacct ggatgcgctt cgacgtatat tgcgggtcaa aaagcacatt
     1321 gatgcccgcc agtttgccga cagtctggta gatgacccgg ctgtcttcta ccgaatgaat
     1381 cgtaatcggc tcattggaga tgggtttcag cgtcggcggg ccgccaatct cctccagtgc
     1441 aggggttgca tttggcgaaa gcgacgtctc ctgggtcggc ggagcattca tcttttcccg
     1501 cgtggcctgg atctcctgct gtgccagttc gttactcggg tcaatctcaa ttgcgtgcag
     1561 aaattccgtg actgcggccg tgtcattgcc ctggtcgcgc aatttctggc ctcgttttac
     1621 atgctcggcg gctgcggcaa agcgggtgcg ctcccaggcc gtcttatagc gcaggtcggt
     1681 cggctttttc tgaaatgcct tgtaatagtc ctcgtaggcc gtctcgatgt cgtctttggc
     1741 ctcggcgttc tggccctgtt taaaccagtg gctcgcggac tgtgcgtgga gcgaagcagt
     1801 gcctaaaaaa agaagaaatc cgaccgaaac gatggccagt tttgcggcgt tgtgtttcat
     1861 gcaactccag gagtcaaaat atccggggaa aagacgtaca gtcgtggccg attataacag
     1921 ggcaccctgt gccccgtgac ctggccctga gccctgtaaa ctaaatgcat ggcccgtcag
     1981 tccagtcatc aggtggcccc tgccgcttcc caaaaatcaa agccgcgaga tccggtggct
     2041 gccggcacac gggacgctgc gcagaaccgc gccgccggac acaactattt cctgctcgac
     2101 aggtttgagg ccggcgttgc tctgcgcggg accgaggtca agtccatccg cgaaggcaag
     2161 gccaatctca aagacgctta cggcatcatc aaagacggcg aggcgtttct gcttaacgcg
     2221 catatcgggc cttattccca caccggatac acgactcatg acgcgctccg cacgaggaaa
     2281 ttgctgctcc accaggaaga aattcgcaag ctacaaggca agacgcagca aaagggattt
     2341 acgctcatcc ccacacgact ttatttccgc aatggccgga taaagtgcga actggcactc
     2401 gccaaaggca aacaggactg ggacaaacgc gaaaccgaac gccgccgcga agccgaccgc
     2461 gaagcaagag ctgcgattgc cagatcacga aaatagcctc cctgcttatg tgactcggag
     2521 ctattttctc aaatccagaa cgctgcgcac ttcagcgttg aacagctcca tcgtccgttt
     2581 caccaacgga tgctcaatcg cctgggcgtg cactgaaccg ccggcggccg cgcgtggttt
     2641 cgcgtttccg ttcgctgtgc cgctctcgcc cggaatgaca gccagcttct gcgttgctcc
     2701 taccgcccgc atcgcgcttt ttgcgatttt ttctgtttcc ggagtcatcg tcaggccgat
     2761 catcgtcttt gttctagcga cctcgacctg aatcgtgtct ccctgaacgg tccagcggcc
     2821 ttcatccaga aatacagcgg cagcattatg ccctttttct tccagagcag cgcaaatgga
     2881 ttcccgaatg cggtcaatct ctgcttctgc atgggatgaa gccacagggg ccagcgccag
     2941 cgacccatcg gtcgcttcga tggccgcttc agcaacacct gtaacagggg ccttcacggc
     3001 ctgcgaagta atctccaccg gagtcagagc acgcagggcc gtaggggctt ccagcttagg
     3061 agaggcggat atttcgctgg tcatttttct ttccctgtct gcctcaaagg gggaaaacgc
     3121 gggtttaggc ggagggccaa caggttgcga agatctcgcc gaagcggcgc cagctttcgg
     3181 caaaacacct ccgcccaacg caggctttcc gccggaaggc atctggctca ggaattcctc
     3241 tatcggcagc aggcgttgca gatggacgag cttaatcagc cccagttcca ggtggaaccg
     3301 ctgctcttgc cggtaattca gctcgtcgta ggtacggagc atgatctgga gaaaacgcgt
     3361 cagatcttct tcagaaaaca gcactgccga ccgggccgcc cgcgccctct catcggctga
     3421 gatttgcaac agttcagaat tctcgcccgc aattcgtgtc atcagcacgt ttctcaggta
     3481 gcggacaaac tgccgcgcca gggcctgtgg actgtttccg gcattgagca aagtattgat
     3541 ttcctcaatg acggcgcttg tgttgttttc gcccaccgct tccagcagcc gctcaaagac
     3601 ggtgttcggc accgtgccca tcagtttgcg gacctgcgcg gcatcaagcc tggcgcgccc
     3661 gtccaccact ggggccgaag caatggcctg gtccatgatc gaaagcgcat cgcgcatcga
     3721 gccgtcccct gcctcagcca gcagcgcgag ggcgtcgtcc tcagcagcga cattttcgtg
     3781 tcctgctata gcgcggagtt ggcccagaat gtcatcaaac ttcaccgcat ggaagctgaa
     3841 gtgctggcac cgcgagcgga tagtctgggg aaagtcctcc ggctgcgtcg ttgccatcat
     3901 aaaaacaacg tggccgggag gctcttccag cgtcttcagc agcgcgttga aggctgcatc
     3961 ggtaatctgg tgcgcttcgt cgaggatgta aatcttgtac ttgtcgcgcg cagggcgata
     4021 gcgggcagca tcacgcagct cgcggatttc atcaataccg cgattggtcg cagcgtcaat
     4081 ttcgatcacg tccactgcat ttccggcgcg aatctccgta cacgagtcgc aaacaccgca
     4141 aggctcagcc gtgggccgct ccggagagcc aatggcagtc cggcaattca gcgccatggc
     4201 cagaatgcgg gcaatggtag tcttcccaat gccgcgatgg ccgctgaaga tgtatccgtg
     4261 ggcaatgcgc ccctgctcaa gggcattgag cagtgtgcgc gtgacatgct cctgcccgac
     4321 aacgtctgaa aaccgttgcg gacggtactt tcgggctaaa acctgatacg ccatgacggg
     4381 acaataatga ggtctgaaag atgattgtaa tcccagcgag ttttcacagc gtgcaaagct
     4441 ctctcaggcc acctcttttt acaatgaaag cgccagagtg gctattcgcc catcggttac
     4501 agtaaaggca tgccgtcaga gcacgatgca ggccggcttt ttgaggaagc cgtcaacatt
     4561 atggcgcgac tgcgcgcccc gggaggctgt ccgtgggacc gcgagcagac ctttgactct
     4621 atccgcaaat acacgctgga ggagacctat gaagtcctgg atacgattga gcggcgcgat
     4681 tggtcccatt tgaaagaaga gctgggcgac ctgcttctgc aggtgctttt t
//
LOCUS       DASRBV010000335        10108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_231672, whole genome shotgun sequence.
ACCESSION   DASRBV010000335 DASRBV010000000
VERSION     DASRBV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231672"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1712
                     /locus_tag="VFA02_12440"
     CDS             <1..1712
                     /locus_tag="VFA02_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HZU10703.1"
                     /translation="YVIGHTAAGVFYGAQTVKQLVSGTGGDAVLHGCVIRDWPAMKYR
                     GVHDDLSRGPVPTLEFQKKQIRTFAAYKINVYSPYFENTFQYASNPLPALPGGSMSQA
                     DAKELVEYAKQYHVTIVPEQEAFGHLHHVLTWQQYAQLAEVPVGAVLAPGEPGSLALI
                     RQWFTELAAVFPGPFLHIGADETFELGQGKTAQDVKQRGLGAVYVDFLKRIHDELAPL
                     HRRLLFWGDVAMNDPEQVKNLPKDMIAVAWHYEPEPEGFSKWLTPYTNAGMETWVAPG
                     VNNWNRVYPNNELALGNIQGFVADGQKAGSTGMLNTIWNDDGEGLFAEDWYGVLFGAA
                     AAWQQGRSDIAEFQKNYGKVFHGDASGDLDQAQLALIAAHQAMEKTGMSDLRDSLFWL
                     DPWSAEGQQVAAKIKPALADVRLDAERALTLIAEARRLGPLREADAIDAMELGARRMD
                     FIAFKFQTASDIAEGYLRLYQNQNDPEVRKHLSRDLWNLSGVNGRCQDLRDGYGYIRD
                     LYKQAWLKENRPYWLDNVLAQYDMAMQLWIARADKIRAARQQWMTEHTLPSPESLGIA
                     QAK"
     gene            1712..3190
                     /locus_tag="VFA02_12445"
     CDS             1712..3190
                     /locus_tag="VFA02_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZU10704.1"
                     /translation="MHIPLRPVDLIIVVLYLIGITWFGLRFRKDGERSIKSYFLADRN
                     IPWWAIALSIVAAETSTLTIISVPGIAFTGDFGFLQLVMGYLLGRIVICIIFLPRYFR
                     GELMTAYQVIGERFGKRLHRTTAFLFLMMRAAAEGVRVFAIAIVVGVALGTGDIASIV
                     ILSLLTLLYTFEGGMKAVIWTDVLQMFLYVLGTLVSLWSISGHIPGGAHTLYAVASSA
                     NKFTVFHFVFSVTEAYTFWAGVLGGCFLTMASHGTDQLMVQRLLAAKNLRESRIALLS
                     SGVVVFVQFSLFLLIGAGLFVYYRTTGQSPHVTSPDRIFPVYIVHQMPAGVSGLMIAA
                     ILAAAMSNLSAALNSLASTSMVDFYLPRKHGLGEAQKTKASRLMTLGWAVVLLVLAIL
                     SRSGGHVLEIGLSIASVLYGGMLGVFLLGTLTRRATETGAMIGLIAGCVLDLLLWRQS
                     APVHLGSSLIVPKIAWTWYVLVGALVTFVIGYLFSGRTRSES"
     gene            3192..5567
                     /locus_tag="VFA02_12450"
     CDS             3192..5567
                     /locus_tag="VFA02_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HZU10705.1"
                     /translation="MRAKGKSLLVVVWLCCSAAFAQQTQKFSTIDQLVGQAIAEHRLP
                     GAVVEIGHNGKVVFRKAYGERSLEPTREPMTADTIFDMASLTKCLMTATATMQLFDQG
                     KLRFNDPVSKYLPEFAANGKEDITIRQLLTHYSGLPPDLSLTEPWEGKQEAFRRAFAI
                     KPDHPAGVRFVYSDINFIVLGALVEKLSDMTEDQYALKNIIQPLGLKHTRYVPPADWL
                     PLIAPTEYEHGVMLRGIVHDPTARRMGGVAGHAGLFSTADDVAIYAQALLDKLAGRPS
                     KFPVSRATLMKMVTPEQPATGTALRGFGWDIESPYSSNRGTIFPVGSFGHTGFTGTSL
                     WMDPVSDTYVIVLANAVHPNGPKSITALRGSIANAAAIALGIAPDNGKLSARLTGYNE
                     SLSGMRRWTARNAEVKTGIDVLEAGNFAELRALAQKHGGKLRIGLLTNQTGVDFAGRR
                     DIDVLAHAEGIELKTIFSPEHGIHGAQDTTNISNSTDTQTGIPVVSLYGATEEQRRPS
                     PEMLRQLDAVVIDLQDAGVRYYTYETAMAYFLEAAGKTGTDIVVLDRPNPINGSFVQG
                     PISDDTESYVHYMPLPVRHGMTMGELARYFNHEHNLHAPLTVVRMQGWQRGDWYDSTG
                     LTWVNPSPNLRDLEEAALYPALGLIETTNISVGRGTDTPFEVFGAPWIHARELAQYLN
                     KRFLPGVRFLPIEFTPQKPYPYGGETCHGVRVLLMDRDVLDSPELGIEIAAALHKLYP
                     ENYKLDRINTLLANQHVLDMLRSGADPQHIAEDWRGPLDKFEKRREQALLY"
     gene            5696..6190
                     /locus_tag="VFA02_12455"
     CDS             5696..6190
                     /locus_tag="VFA02_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZU10706.1"
                     /translation="MSLKIMSTDLKQQFMKRAISLATDNVRNNTGGPFGAVIVKDGRI
                     VGEGVNKVTATNDATAHAEIVAIRNACRNLNTFRLDGCELYTSCEPCPMCLAASYWAH
                     ITAIYFGNRAEDAARIHFADAFLYEQLALPREKRAVPEHEMMREQAWESFQAWIESPS
                     KVEY"
     gene            complement(6307..7638)
                     /locus_tag="VFA02_12460"
     CDS             complement(6307..7638)
                     /locus_tag="VFA02_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU10707.1"
                     /translation="MPDAVQATKQYNYGTWRFQKGWTPLHIVDAEGCYIIDATGKKYL
                     DFSSQLMCVNLGHRNKAVVDAIAEQARTLAYAMPSYATTARAELSAALTEVLPDGLTK
                     FFFTTSGTEANEAAFKIARMYTGRSKIVARYRSYHGSTAASIAATGEARRWASEPSSK
                     GRDFLFAPEVNCYKCPLKHTYPECGIACVDYLEHIVDNEHDVAAVIVEPIVGTNGVLV
                     PPPEYMPRLRQICDRHGALLIADEVMTGWGRTGKWFAVDHWNVKPDILTTAKGITSAY
                     VPLGLCATTEKIAAYFDDHYFAHGHTYEAHPLTLKPAVATISEMKRLKLVERAAELAP
                     YVQTKLNALRENHPCIGDVRGMGLFWALDLVKDRTTKQPFNTMGDKISGKPLVVDQIA
                     AQMMKNGVTIQAWVSHLIVAPPLIIEREQIDTGIAALDEALKIADALVTHP"
     gene            complement(7647..9092)
                     /locus_tag="VFA02_12465"
     CDS             complement(7647..9092)
                     /locus_tag="VFA02_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZU10708.1"
                     /translation="MKDTGFSGCRLMGMILLAFSFCASPFAGQAATRHDPRLEGAYSF
                     ERNGWTYIHLQGTPEQVGYQHGYLLADKIEDALHVFRLEDEHSTGRDWTFFRNAAKTV
                     LWPHIDPEYQQEMSAIAKGAQAKGVHVDVWDIVALNGTMELAEYYVPWLNRQQHAKHA
                     PKVDAPGNCSAFIATGSYTKGGEIVMAHNNWSSYAEGERWTMVFDIEPAKGHRILMDG
                     VPGMITSNDDFGINDAGIMLTETTITQFEGWNSNGSPTFMRSRKALQYASSIDEVVAT
                     MRNGNNGGYANDWLIGDRKTGEIAYLELGLKNTPLWRTKDGYFVSSNFARDPKLIQEE
                     TSGFNYNDLSTSPNARRVRWEQLMSNYKGKIDADAAGRFLSDHEDSFLKKENPDQRSL
                     CGHVDTVKEGIPQWDWPPYYPGGAVQGKVTTSDLAARMSIIAHAGHPCGEDFLAKPFL
                     AAHPEFSWQAPILRDMKSGPWTTFTAGEKAN"
     gene            complement(9138..>10108)
                     /locus_tag="VFA02_12470"
     CDS             complement(9138..>10108)
                     /locus_tag="VFA02_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU10709.1"
                     /translation="QSTRLHLTAGKAVHVQFDYVPDGNHVLAGVGIIAVDDLVSPEAK
                     KIVSMADAALVSVGFDPSTESEGMDRTFALPWGQDELIEAVAAANKNTIVAITGGGGV
                     DMRGWIDKVPALLHNWYPGEEGGTALAEIVFGKRSPEGHLPASFERAWEDNPVHDSYY
                     APPVPEGQTPHVKYSEGVFVGYRYYTSRNKKPLFPFGFGLSYTTFRFSNLEISPKQAS
                     SDGNIMVSFDVTNTGQREGAEVAQVYVGDPSAKVERPVKELKGFEKVRLNPGEAKHVS
                     VTLDRRALAYWDTNANDWRVDPGEFKVYVGDSSENTPLSGEFTVAK"
BASE COUNT         2151 a         2800 c         2828 g         2329 t
ORIGIN      
        1 tctacgtgat tggacatacg gccgcaggcg tcttctacgg cgcgcagacc gtgaaacagc
       61 tagtttccgg aacaggcggt gacgcggtgc tgcatggatg cgtcattcgc gattggccgg
      121 caatgaaata tcgcggcgtg cacgacgatc tctcgcgcgg accggtgccg acgctggagt
      181 tccagaaaaa gcagattcgc acctttgctg catacaaaat aaacgtgtat tcaccttact
      241 ttgagaacac gtttcaatat gcttcaaacc cgctgcccgc attgcctggc ggttccatgt
      301 cgcaggccga tgcgaaagaa ctcgtggagt atgcaaagca atatcatgtg acgattgttc
      361 cggaacagga ggccttcggg catctgcatc acgtgcttac ctggcagcag tatgcgcagc
      421 tggccgaagt accggtgggg gccgtgctcg ctccgggaga gccgggctca cttgcactca
      481 tccggcagtg gtttactgaa ctggccgctg tctttcccgg accatttctg cacatcggag
      541 cagacgagac gttcgagttg gggcagggca agacggcaca ggacgtaaag cagcgcggac
      601 tgggcgcagt ctatgtagac ttcctgaagc gcattcacga tgagcttgcg ccgctccatc
      661 gcaggctgct cttctggggc gatgtggcga tgaatgatcc ggaacaggtg aaaaacctgc
      721 cgaaggacat gatcgcggtg gcctggcact atgagccaga gccggaaggc ttcagcaagt
      781 ggctaacgcc ctatacaaat gccggcatgg agacctgggt cgctcccgga gtgaacaact
      841 ggaaccgcgt ctatccgaac aacgagctgg cgctcggtaa cattcagggc tttgtggcgg
      901 acgggcagaa ggcgggaagc accggaatgc tgaacaccat ctggaacgat gatggtgagg
      961 gcctgtttgc ggaagattgg tatggggtgc tgttcggcgc agcggctgcg tggcagcagg
     1021 gaagaagcga cattgctgag ttccagaaaa actacggaaa ggtctttcat ggtgatgcct
     1081 ccggagatct tgaccaggcg cagcttgcat tgattgccgc gcatcaggca atggaaaaga
     1141 ccggcatgag tgatttgcgt gattcgctgt tctggctgga tccgtggagc gcggaaggtc
     1201 agcaggtggc agccaaaatc aaaccagcac tggcagatgt tcgtctggat gccgagcgcg
     1261 ctctgacgct gattgccgag gccaggcggc tgggcccact gcgtgaagcc gatgcaatag
     1321 acgcaatgga actgggcgcg cggcgcatgg actttatcgc attcaaattt cagacggcca
     1381 gcgatattgc cgagggctac ctgcggcttt atcagaacca gaatgatccg gaagtgcgta
     1441 agcacctctc gcgcgacctg tggaacctct ccggtgtaaa cggccgctgc caggacctgc
     1501 gcgatggcta tggctatatc cgtgatctat acaagcaggc atggctcaaa gagaaccgtc
     1561 cttactggct cgacaatgtg cttgctcagt acgatatggc catgcaactt tggattgcgc
     1621 gcgcggacaa aattcgcgct gcccgccaac aatggatgac ggaacatacc ttgccatcac
     1681 ctgaatctct tggaatagcg caggcgaaat aatgcacata ccgctgcgtc ctgttgatct
     1741 gatcattgtt gttctgtatc tcatcgggat tacctggttt ggcctgcggt tccgcaagga
     1801 cggcgagcgc tccatcaaga gctactttct tgccgaccgg aacattccct ggtgggcaat
     1861 tgcgctctcc attgttgccg ccgagaccag cacacttacg atcatcagcg tccccggcat
     1921 tgcctttacc ggcgatttcg gctttctgca actggtgatg ggctatctac tgggacggat
     1981 tgtcatctgc atcatctttt tgccgcgcta ttttcgcgga gagctgatga cggcgtatca
     2041 ggtcatcggt gagcggttcg gaaagcgcct gcaccggaca acggcgttcc tctttctcat
     2101 gatgcgcgcg gcagccgaag gggtgcgcgt ctttgcgatt gcgattgtgg ttggcgttgc
     2161 gctgggaaca ggtgacattg cctccattgt gattttgtca ctgctgacgc tcctctatac
     2221 ctttgaaggc ggcatgaagg cggtcatctg gacagatgtt ttgcagatgt ttctttatgt
     2281 attgggtacg ctcgtaagcc tgtggagcat cagcggacac attcccggcg gcgcgcacac
     2341 gctgtatgca gtagcgtcgt ctgcaaataa attcacggtc tttcattttg tcttttctgt
     2401 aactgaagcg tatacattct gggcgggcgt gctcggcggc tgcttcctta cgatggccag
     2461 ccacgggacc gaccagctga tggtacagag attgctcgcc gcaaaaaacc tgcgcgaatc
     2521 acggattgcg cttctcagta gcggcgttgt tgtctttgtg cagttctcgt tgtttctgct
     2581 gattggcgct gggctgtttg tgtattacag aacaaccggg caatcaccgc atgtaaccag
     2641 cccggaccgt attttccctg tctatattgt ccaccagatg cctgcgggag tttccggtct
     2701 gatgattgct gcgatccttg cggctgccat gtccaacctg agcgcggccc tcaattctct
     2761 ggcttccact tccatggtgg acttttatct tccccgcaaa cacggtctgg gcgaagcaca
     2821 gaaaacgaag gcctcacgcc tgatgactct gggttgggcc gtggtccttc tcgtcctggc
     2881 gattctctcc cgcagcggcg gccatgtgct tgaaattggc ctgtccattg cttcagtgct
     2941 ttatggggga atgctcgggg tatttctgct gggaacactg acgcgtcgcg caactgaaac
     3001 cggggcaatg attggcttga ttgcaggatg cgtgctggat cttttgttat ggcggcagag
     3061 tgcgcccgtc catctgggaa gctctttgat tgttcccaag attgcatgga cctggtatgt
     3121 cctggtcggt gcgctggtaa cgtttgtgat tggatatctt ttcagtggcc gtacaaggag
     3181 cgaatcgtaa gatgcgtgcg aaagggaagt ctttgctcgt ggtggtatgg ctctgttgtt
     3241 cagctgcttt tgcgcagcag acgcagaagt tttcaacgat tgatcaatta gtaggtcagg
     3301 ccatagcaga gcaccgcctt cccggcgcag ttgtcgaaat cggccacaat ggcaaagtcg
     3361 tttttcgcaa ggcctatgga gagaggtcgc ttgaacccac tcgcgaacca atgactgcgg
     3421 acaccatctt tgacatggca tcgctgacga agtgcctgat gacagcgacg gccacgatgc
     3481 agctcttcga ccagggcaaa ctgcggttca acgatccagt gtcgaagtat ctgccggagt
     3541 ttgccgcgaa cggtaaggaa gacatcacga ttcgccaact ccttacgcat tattctggcc
     3601 ttccgccaga cctttctctg acggagccgt gggaagggaa gcaggaggca ttcaggcgcg
     3661 catttgccat caagccagac catcctgcag gtgtgcgctt tgtttacagc gacatcaact
     3721 tcatcgtgct tggcgctttg gtggagaagc tgtcagacat gacggaagac caatacgcgc
     3781 tcaaaaacat tatccagccg ctggggttaa agcatacgcg ctacgtacct cctgcagatt
     3841 ggctgccgtt gattgctccc acagaatatg agcacggagt gatgttgcgt ggcattgtgc
     3901 atgatccaac cgcgcgacgc atgggcggcg tcgcagggca tgcagggctt ttcagtacag
     3961 cggacgatgt ggccatctac gcacaggcat tgctggacaa actggcaggc aggccgagta
     4021 agttcccggt ttctcgcgcc actctcatga agatggtcac gccggaacag cctgcaacag
     4081 gtacagctct gcgcggtttt ggctgggaca tcgaatctcc atactcgagc aatcgcggaa
     4141 cgatctttcc tgttgggtca tttggccaca ccggatttac ggggacttcg ctgtggatgg
     4201 acccggtctc agacacatat gtgattgttc ttgcgaacgc cgtccatccc aacggtccaa
     4261 agagcatcac agccctgcgt ggaagcattg ccaacgcggc cgcaattgca ttgggaatcg
     4321 ctccagacaa tggaaagctg tctgccaggc tgaccggata taacgaatcg ctcagcggta
     4381 tgcggcgctg gacagcacgc aatgccgaag tgaagacagg catagatgtt cttgaagccg
     4441 gcaattttgc cgaacttcgc gcgctcgcac aaaaacatgg cggaaagctg cgtattggtc
     4501 tgctcacaaa tcaaaccgga gtagactttg cgggccggcg cgatattgac gttcttgccc
     4561 atgctgaagg aatcgagcta aagacaattt tcagtcctga gcacggaatc cacggcgcgc
     4621 aggacacgac aaatatcagc aactcaaccg atacgcagac ggggattcct gtcgtaagcc
     4681 tttacggcgc aacagaagag cagcggcggc cttcgcctga gatgctgcgc cagctcgatg
     4741 ccgtcgtgat tgatttgcag gatgcgggtg tccgctacta cacctatgaa actgcgatgg
     4801 cgtatttcct ggaagccgca ggaaagacgg gaacagatat tgttgtgctc gaccgcccca
     4861 atccgatcaa cggatcattt gtgcaggggc caatctccga tgacaccgag agttacgtcc
     4921 actacatgcc tctgcccgtg cgccacggca tgacgatggg tgagctggcg cgttacttca
     4981 accacgagca caatctgcac gcgcctctga ccgtagtcag aatgcaggga tggcaacgcg
     5041 gcgactggta cgattccaca gggctgacat gggtcaatcc ttctccaaat ctgcgcgatc
     5101 tggaagaagc agcgctctat cccgctttgg ggctgataga aacgaccaat atctccgtcg
     5161 gtcgtggtac agatactcca ttcgaggtat tcggcgctcc gtggatccat gcgcgcgagc
     5221 ttgcgcaata tctgaacaag cgttttctgc ctggagtacg tttccttccc atagagttta
     5281 ccccgcaaaa gccttatcca tatggcggtg aaacatgcca tggcgtgcgt gtgctgctaa
     5341 tggaccgcga tgttctcgat tcgccggaac tgggcataga gatagctgcg gcgttgcata
     5401 aactttatcc ggaaaactac aagctcgacc ggatcaatac gctgcttgcc aatcagcatg
     5461 tgctcgatat gcttcgcagc ggggccgatc cgcagcacat tgcagaagac tggcggggtc
     5521 cgctggataa gtttgaaaaa aggcgggaac aggcattgtt atattgaccc ggcggtcttt
     5581 tgacaacaaa gcagatcgcc cttgcgagcg caaagaccat ggctgcaggc cccggaacac
     5641 gcaggacggc cacaggatat gcccgaatgt gagtgcgggc acgtaagata gttgtttgag
     5701 tctgaagatt atgtccactg atctgaagca gcaattcatg aagagggcga tttcactcgc
     5761 aactgacaat gtacgcaaca acaccggagg cccattcggt gcggtcatcg tgaaagatgg
     5821 ccggattgtc ggggaaggcg ttaataaggt gaccgcaacc aacgatgcaa ccgcgcatgc
     5881 tgagattgtg gccattcgca acgcctgccg caacctcaat acgttcagac tggacggctg
     5941 tgagctttat acgagctgtg agccttgccc gatgtgtctg gctgcatctt attgggcgca
     6001 catcacggcc atttatttcg gcaaccgcgc cgaagatgcc gcgcgcattc acttcgccga
     6061 cgcattcctg tatgagcagc ttgcgcttcc gcgtgaaaag cgcgcggttc ctgagcacga
     6121 gatgatgcga gagcaagcct gggagagttt tcaggcctgg attgaaagtc cttcaaaggt
     6181 cgaatactga ccggtcccca tcctttcgcg tacaaggcgc gaaatcagcc aaggcgctct
     6241 ccttcaggtt ttcgcttgcg tatgttctat tcagaaggag ggcggccgga aggctgatgg
     6301 tgtcttctat ggatgcgtga ccagagcatc agcaatttta agcgcctcat ccagagcggc
     6361 aatgcctgta tctatctgtt ctctttcgat aatcaatggc ggggcaacga tgagatggct
     6421 gacccacgcc tggatggtca ctccattttt catcatctgg gctgcaatct ggtctacaac
     6481 caggggctta ccgctgattt tatcgcccat agtgttaaaa ggctgcttcg tagtacggtc
     6541 ttttaccaaa tccagggccc agaagagtcc catgccacgc acatcgccaa tacacgggtg
     6601 gttttcccta agggcgttga gttttgtttg cacatagggc gccagttccg cggcgcgctc
     6661 gacaagtttc agacgcttca tctcagagat ggtcgccact gccggcttca acgtcagcgg
     6721 atgcgcttcg tacgtatgtc cgtgcgcaaa ataatgatca tcgaagtatg cggcgatttt
     6781 ctctgttgtc gcgcagaggc caagaggtac ataagcagag gtgatacctt tcgccgtggt
     6841 caggatgtcg ggtttgacgt tccaatggtc taccgcaaac cacttgccag tgcggcccca
     6901 tcccgtcatc acttcgtcgg cgataagcag cgcaccgtgg cggtcacata tctgtcgcag
     6961 acgcggcatg tattccggcg gaggaacgag caccccattg gtcccgacga taggttctac
     7021 gatgacagcc gccacatcat gctcattgtc aacgatgtgc tccagataat ccacgcatgc
     7081 aatgccgcat tccggatatg tatgcttcag cggacactta tagcagttca cttccggcgc
     7141 aaagagaaag tcgcgtcctt tactggaagg ttcggaggcc cagcggcgcg cttctcctgt
     7201 ggcagcgatt gaggcggcag ttgagccgtg ataagagcgg tagcgggcca caatcttcga
     7261 gcgtcccgtg tacatgcggg cgatcttgaa tgcagcttca ttggcttcag ttccggatgt
     7321 ggtgaagaag aactttgtca gcccatcggg caggacttcc gtcagggccg cactcagttc
     7381 cgcgcgcgcc gttgttgcat aggaaggcat ggcataagcc agtgtgcgtg cctgttcggc
     7441 aatggcgtcc accacagctt tgttcctgtg gccgagattt acgcacatca gctgcgatga
     7501 gaagtcgagg tacttctttc ccgttgcatc tatgatgtag cagccttcgg catcaacaat
     7561 gtgcagcggt gtccaccctt tctgaaaccg ccacgttccg tagttgtact gtttcgtggc
     7621 ttggacagca tcaggcatgg tttccctcag tttgcttttt ccccggcagt aaaggtggtc
     7681 cacggcccgg atttcatgtc ccgcaggatg ggcgcctgcc aggaaaattc aggatgcgca
     7741 gcgagaaatg gctttgcaag aaagtcttcc ccgcagggat gtccggcatg ggcgatgatg
     7801 ctcatcctgg ctgccagatc gctggtggtc actttgccct ggactgcgcc gcctggataa
     7861 tagggtggcc agtcccactg cggaatccct tccttcactg tatccacgtg cccgcaaaga
     7921 ctgcgctggt caggattttc cttcttcagg aaactgtctt catgatcact gaggaagcgg
     7981 cctgcggcat cggcatctat cttaccttta tagttggaca tgagctgctc ccagcgcact
     8041 cggcgcgcgt tgggtgaggt ggagaggtcg ttgtaattga aaccgctcgt ctcttcctgg
     8101 atgagcttcg ggtcgcgcgc aaaattggag ctgacaaagt agccgtcctt cgtgcgccac
     8161 aacggagtgt ttttcagtcc cagttcaaga taagcaatct ctccggtttt ccggtcacct
     8221 atgagccaat cgtttgcata tccgccattg ttgccgttgc gcatcgttgc cacaacctca
     8281 tcaatggagc tggcgtattg cagtgctttg cgtgagcgca tgaaagtggg gctgccgttg
     8341 gagttccatc cctcaaattg tgtgatggtg gtttcagtca acatgattcc ggcgtcgtta
     8401 atgccgaaat cgtcattgct ggtgatcatt ccgggaacgc cgtccatcag aatgcggtgg
     8461 ccttttgccg gctcaatgtc aaagaccatc gtccagcgct cgccttcggc atacgaactc
     8521 cagttattgt gcgccatgac gatttccccg cctttggtat agcttcctgt tgcgatgaag
     8581 gcactacaat ttcccggagc atccaccttc ggcgcgtgtt tggcgtgctg ctgtcgattg
     8641 agccagggaa cgtagtactc cgccaattcc atcgtgccat tcaacgccac gatgtcccag
     8701 acatccacat gcacgccttt ggcctgtgcg ccctttgcga tggccgacat ctcctgctga
     8761 tattccggat ctatatgcgg ccagagtacc gtcttcgccg cattgcggaa gaaggtccag
     8821 tcgcgtccgg tgctgtgctc gtcttcaagc cggaaaacgt gcagcgcgtc ctcaatttta
     8881 tcggcgagca gatagccgtg ctgatagcca acctgctccg gagtaccctg aaggtggatg
     8941 tatgtccacc cgttgcgctc aaagctgtag gccccttcca gccgaggatc atgtcgggtc
     9001 gcggcctggc ccgcaaaagg actcgcacag aaactgaatg ccagcagaat catgcccatc
     9061 agacgacagc cagaaaagcc agtgtctttc atgccaacgt tctccatgga aaagaagttg
     9121 cttacgaccc gccgagacta tttcgccacg gtaaactccc cactgagcgg agtgttctct
     9181 gaggaatccc ccacatacac cttaaactca cccgggtcta cgcgccagtc attcgcattc
     9241 gtgtcccagt aagcgagcgc gcgccggtcc agcgtcaccg aaacatgctt cgcctcacca
     9301 ggattcaacc gcaccttctc aaagcccttc agttccttta cgggacgttc caccttcgcc
     9361 gacgggtccc ccacatacac ctgtgccacc tcggccccct cgcgctgtcc cgtgttcgtc
     9421 acgtcaaagg aaaccataat gttcccatcg ctcgacgcct gcttcggaga aatctccagg
     9481 ttgctgaacc ggaacgtcgt atacgacagc ccgaatccaa acggaaacag cggtttcttg
     9541 ttccggctcg tgtagtagcg atacccaaca aacacgcctt cgctgtactt cacatgcggc
     9601 gtctgtcctt ccggaacagg cggagcgtag taggaatcat gcaccggatt gtcctcccag
     9661 gcgcgttcaa agctcgcagg caaatgtccc tctggcgacc gcttgccgaa gacgatctcc
     9721 gccagcgccg tgccgccttc ttcacccgga taccagttgt gcaacagcgc tggaacctta
     9781 tcaatccatc cacgcatgtc cacaccgcct ccgcccgtga ttgcgacaat cgtgttcttg
     9841 ttcgctgcag ccacggcctc aatcagctcg tcttggcccc agggcagcgc gaaagtgcgg
     9901 tccatgcctt cactttccgt agaaggatca aatcccaccg agaccagcgc cgcatcagcc
     9961 atagagacga tcttctttgc ctccggtgag accaggtcat ctactgcgat gatgccgact
    10021 ccagccagca catggtttcc atccggcaca taatcaaact ggacatggac cgccttgccc
    10081 gccgtcagat gcagcctggt ggactgcg
//
LOCUS       DASRBV010000336         8932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_538886, whole genome shotgun sequence.
ACCESSION   DASRBV010000336 DASRBV010000000
VERSION     DASRBV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538886"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFA02_12475"
     CDS             <1..184
                     /locus_tag="VFA02_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10710.1"
                     /translation="QDYPMPEATTAANRPAAWLEKTEGLKFQTVGQSKQTSLIPLYQL
                     LDQRYSVYWKVNTKSA"
     gene            186..1952
                     /locus_tag="VFA02_12480"
     CDS             186..1952
                     /locus_tag="VFA02_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10711.1"
                     /translation="MTDPFSRRDLLKGGVALVAATRLGASTFSQDLFAQEVKTSQITP
                     LTSTSGVYIPPRGESFFKFSYDFPEPSVEFAGLQFGFRVFTFENTYGLDAAHIRVLAV
                     SDGLEIECSQFVWAGGQMNAPGHLKAIIRKHGDDITWQASAEMDRPIKSIATVLRWVP
                     RGRISASANQFFDPKDNEVLLQYPYLGGGLTTPLVIIEKSPQQYFVLSAEEEKVRGVR
                     YYLQPGDAGYRTELIYEAAGWERSNSITSCRWRIGYASSVEAAARPHYAHLEKAFGIP
                     DWETRADVPSWMRQTDLVLALHGTHWTGYIFNDFAKMLEVLKWTNTQIPGRNVLVFLP
                     AWDGRYYWNYPIYKADPRLGGEDGLRRLVDEGHKMGFRFMPMFGTNSANKTLPDFRNF
                     SDATTDLIDGNAYDLDWVDWDNDRGMEGWSPFMNVGVASWRNWLSARISEVIEKYNVD
                     AYFLDIVGGWQNNSKADMFEGTRELIADLRRKHPGIVPVGEMHYDALMSCIPMYQVNS
                     ASLYHPGFRKYVRTYEHLSRPAPGRGSTGVHEAGFGHFYPHVDPKQFVIPTITVVDDT
                     FDKYRDVMAQVIQGAKIRAGLS"
     gene            complement(2007..3593)
                     /locus_tag="VFA02_12485"
     CDS             complement(2007..3593)
                     /locus_tag="VFA02_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HZU10712.1"
                     /translation="MLNGTSILGPKRGSKTGEVFYGINPATGEQLQPAFFSASLEDID
                     IAAKLADAAFASYSRLSNSKRAAFLRSIADALQKVSAEIIGRAHLETALPLPRLEGEL
                     ARTANQLRLFADVVEEGSWVNARIDTADPERKPLPKPDVRSVLQPLGPVAVFGASNFP
                     LAFSVAGGDTASALAAGCPVVVKAHPAHPGTSELVGNVIRECVAAAGLHKGVFSLLFD
                     SGVEVGTHLVQHPLIKAVGFTGSLKAGRALMGLCASRPDPVPCYAEMSSTNPVFVLPG
                     AINQYDKIAADLTASYTLGAGQFCTKPGMVFLPPQNESSALVEELRKRAASLPAFTML
                     TPGIAAQYKRGLMQRTESDGARILAASEASATAPAGTAAVLLEAEAEDLLNKPELAEE
                     IFGPTTLLVRYQSREQMLKVAESLQGHLTATLLGTEEDLSSHQDLIAILERKVGRVIF
                     NGYPTGVEVCHAMVHGGPYPATSDSRSTSVGTLAIYRFARPVCYQNMPQSALPEALQN
                     NNPLGLLRMVNGKMTRESVG"
     gene            complement(3720..4646)
                     /locus_tag="VFA02_12490"
     CDS             complement(3720..4646)
                     /locus_tag="VFA02_12490"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZU10713.1"
                     /translation="MNWFGVMPAMTTAFDEQLKVDHAFMQQHAQWLLDNGCTGLVMLG
                     SLGEAATLTFDEKIAILKNMVATANGKAPIVAAISALSTAEAVSLAKAAADAGCSGLM
                     VLPPYVYRGDWREMKAHIAAVFKAAPLSCMLYNNPVAYGTDFLPKHIAELAAEHSNFH
                     SVKESSTDVRRVTAIRALVGDRLKIFVGVDDAIVEGIAAGACGWIAGLVNAMPAESVA
                     LFNFAYEGKTKDAFELYRWFLPLLRMDTIPKFIQLIKFVQQELGVGSARVRPPRLEIT
                     GEELAETKRTIAEALKQRPEIKEVVFAAASAH"
     gene            complement(4643..5914)
                     /locus_tag="VFA02_12495"
     CDS             complement(4643..5914)
                     /locus_tag="VFA02_12495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZU10714.1"
                     /translation="MIQRFDIVVVGGGPAGMAAVACAAEGGRHVAVVDDNFTLGGQIW
                     RGKDTPLKTAEVWRARVAAANVVRIQGARVYAQPAPGVLRAEQNGSYVDVAYEKLILA
                     TGARERFLPFPGWTLPNVAGAGGLQALVKNGLFVEGKRIVVAGSGPLLLAVAAYLKRH
                     KAKVLAICEQAPLSRLLKFATSIVTDKEKLLQAVVYKSQTLSAPFHTGCWPVSAQGKE
                     RLESVTLRKGNRTWTIACDYLACGFHLVPNTEVPELLGCRVLDGKVAVDEWQQTSILD
                     IYCAGEPTGVGGVELALVEGQIAGYAAAGLPDNARSLFAQRARQRKFAQKLEEAFALD
                     SALKELPQDDTVICRCEDVSYLTLKDEPSWRAAKLHTRCGMGPCQGRICGAATEFLFG
                     WNAGSVRPPIFPATLSCLEAIPELLELQETR"
     gene            complement(5911..6153)
                     /locus_tag="VFA02_12500"
     CDS             complement(5911..6153)
                     /locus_tag="VFA02_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU10715.1"
                     /translation="MPERITLTINGRWCEALEGTTVAAAIMNAGAACRASATGELRGP
                     LCGMGICFECRAIVNGKPHQRTCQMLCEAGMEVNTQ"
     gene            complement(6146..7270)
                     /locus_tag="VFA02_12505"
     CDS             complement(6146..7270)
                     /locus_tag="VFA02_12505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU10716.1"
                     /translation="MISTYDAVVIGAGIVGAACACELARAGMRTAIVEASIVGGGATA
                     AGMGHIVVMDDSPAQLALTQYSQSLWNEIAPQLPANVEYDRCGTLWIAADDEEMQEVR
                     RKQAVYASVNVRTEVLDSRALSEAEPNLRRPLAGALLVPSDAVLYPPCAAVYLLECAK
                     QAGAALYIGNAVAKAERGRVTFSDGTELSAALIIHATGAGATRLMPHLPIRKRKGHLV
                     ITDRYPDFVRHQLVELGYLKSAHSITSDSVAFNVQPRRTGQLLIGSSRQYDNEGAGVD
                     QKVVSAMLARAFDYIPSMKELSSIRVWTGFRAATPDKLPLIGPTEDPSVFLATGHEGL
                     GITTSLATARLLTDHLLQRGTSIPLGPYLPERFAREVIYA"
     gene            complement(7267..8223)
                     /locus_tag="VFA02_12510"
     CDS             complement(7267..8223)
                     /locus_tag="VFA02_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HZU10717.1"
                     /translation="MLTSDTSHRVQVIDSHTAGEPTRLVLSGGPDLGSGPLSGRLERF
                     RAQHDTFRSAVVNEPRGSDVLVGALLCEPYDPSCDIGVIFFNNVGYLGMCGHGSIGVI
                     VSLAWLGRIGPGTHRIETPVGVVNATLHSSGRVTIANVPSYRSESGVTVDVPGFGKVT
                     GDIAWGGNWFYLVEDHRMPLGLQHVGELTDFTWAIRQSLNKNGITGNDGAEIDHIELF
                     GPPSNGHAHSRNFVLCPGKAYDRSPCGTGTSAKMACLYADGKLAPGDIWRQEGILDTV
                     FEGSIEVRDGLVLPSITGEAYVTAESSLVLDANDPFRMGIVP"
     gene            8349..>8932
                     /locus_tag="VFA02_12515"
     CDS             8349..>8932
                     /locus_tag="VFA02_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU10718.1"
                     /translation="MTPLKKTVSRSSKAEHGSSLRTAFHEIRELIVHGRLSPGTWIVE
                     GDLAARLGMSRTPVRGALHWLQREGYVLEHKNVSKSRMIVAPLTKEDANELYSIIGRI
                     EGLAGRRTASLPQAKRTEIAQQLTHYNHQLEEIVKARPAKTGGIFDLDHNFHRAIVEA
                     GAGPRLLALHKAIEPQTERYWRLYASSIIHDLHRS"
BASE COUNT         1982 a         2503 c         2504 g         1943 t
ORIGIN      
        1 ccaggactat cccatgcccg aagcaacaac tgcggcgaat cgtcctgcgg cgtggcttga
       61 aaaaacagaa gggctcaagt tccagaccgt aggccagagc aagcagacat cgctcatacc
      121 gctctatcaa ttgcttgatc agcggtacag cgtctactgg aaagtgaata caaaatcggc
      181 atagtatgac tgatccattt tcccgtcgtg accttttaaa gggtggggtg gccctcgtcg
      241 cggccactcg ccttggcgca tccacttttt ctcaggacct ctttgcgcaa gaagttaaaa
      301 ccagtcagat aacaccgctg acttctacca gcggcgttta cattcccccg cgcggcgaga
      361 gtttcttcaa gttcagctat gactttcccg agccgtcggt cgaatttgca ggattgcagt
      421 tcggtttccg tgtcttcact tttgaaaata catatgggct ggatgcagcg cacatccgcg
      481 tgctcgctgt gagtgatggc ttggagatcg aatgctcgca gttcgtctgg gccggcggcc
      541 agatgaacgc gcctggtcat ctgaaggcca tcattcgcaa gcatggtgat gacataacgt
      601 ggcaggccag cgcggagatg gaccgcccta tcaagtccat cgccacagtt ttgcgctggg
      661 tcccgcgcgg aaggatctca gcatcggcaa accagttctt tgatccaaag gacaatgaag
      721 tcctgctgca atatccctat ctcggcggag gactgacgac gccgctggtc atcatcgaga
      781 aatctccgca gcagtatttc gttctttcgg cagaagaaga gaaggtgcgc ggcgtgcgct
      841 attatctcca acccggagat gcaggatacc gcaccgaact gatctacgaa gcagcagggt
      901 gggagcgcag caacagcatc acaagctgcc gctggcgcat tggttatgca tcgagcgtgg
      961 aagcagcggc acgtccgcac tatgcgcatc ttgaaaaggc ctttggtatt cctgactggg
     1021 agacgcgcgc cgacgttcct tcgtggatgc ggcagacgga ccttgtgctc gccctgcatg
     1081 gcacgcactg gacgggctac atcttcaacg attttgcgaa gatgctcgaa gtcctgaagt
     1141 ggaccaacac gcagattccc ggacggaacg tgctcgtctt tctgcccgca tgggacggcc
     1201 gttattactg gaactatcca atttataagg ctgatcctcg tctgggagga gaagacggtc
     1261 ttcgccgctt agtagacgag ggccataaga tgggcttccg ctttatgccg atgtttggca
     1321 cgaattctgc gaacaagacg ctgccggatt ttcgcaactt cagcgatgcg acgaccgacc
     1381 tgattgacgg caatgcctac gatcttgatt gggtggattg ggacaatgac cgcggcatgg
     1441 aaggctggag ccccttcatg aatgttggtg tggcttcgtg gcggaactgg ctttccgcgc
     1501 gtatctccga ggtcatcgag aagtacaacg tagacgcgta tttcctggac attgttggcg
     1561 ggtggcagaa caactcgaag gctgacatgt ttgagggtac gcgcgagctg attgcagacc
     1621 tgcgtcgtaa gcaccccggc attgttccgg tgggcgaaat gcactatgac gcgctgatgt
     1681 cttgcatccc gatgtatcag gtaaactcag ccagcctgta tcatccgggc ttccgcaaat
     1741 atgtgcgcac gtatgaacac ctgagccgcc ctgctcccgg acgcggaagc accggcgtgc
     1801 atgaggccgg cttcgggcac ttctacccgc atgtagaccc gaagcagttt gtgattccga
     1861 cgattactgt ggtggacgat acatttgata agtatcgtga tgtgatggcc caggtgatcc
     1921 agggagcaaa gatcagggcc ggattaagct aaaggatagg ttgtcattct tccctgcaat
     1981 cttcagaatg acaaactgta attcgttcat ccaacgcttt ctctggtcat ctttccgttg
     2041 accatgcgca gcaggccgag cggattgttg ttctgcaaag cttcaggcag cgcagactgc
     2101 ggcatgttct ggtagcagac agggcgcgca aaacgataga ttgccagggt gccgacagaa
     2161 gtggagcggc tgtccgacgt agccggatag gggccgccgt gaaccatagc atgacagact
     2221 tctacgcccg tgggatagcc attgaagatg acgcggccga ctttccgttc cagaattgca
     2281 attaaatcct ggtgggagga aaggtcttct tccgtgccga gaagcgttgc cgtcaggtgc
     2341 ccctgcaatg attcagcaac cttaagcatc tgttcacggc tttgatagcg gacaagcaat
     2401 gttgtggggc cgaagatttc ttctgccagt tcaggcttgt tgagaaggtc ctcggcctca
     2461 gcttcgagca ggacggcggc cgtgcccgcg ggcgctgttg cgctggcttc cgatgcagca
     2521 agaatacgcg cgccatcgga ttcagttcgc tgcataagtc cgcgtttgta ctgcgccgca
     2581 atgcctggag tgagcatagt gaatgcgggc agagatgctg cgcgtttacg cagctcttcg
     2641 acgagcgcag aagattcgtt ctgcggcggg agaaagacca tgcccggttt ggtgcaaaac
     2701 tggcctgctc ccagcgtgta ggaggcagtg aggtcagcag caattttgtc gtattgattt
     2761 attgctccgg gcagaacaaa gaccgggttt gtgctgctca tctctgcata gcagggaacc
     2821 ggatctggac ggctggcgca taaacccatc agtgcgcggc ctgctttgag agagccggta
     2881 aatcctacgg ctttgatgag tgggtgttgc acaagatgcg tgcccacttc gactccggaa
     2941 tcaaaaagca gcgagaagac gcctttgtgc agtccggcag cggccacaca ttcacggatg
     3001 acattgccta caagctcact tgtacctgga tgcgctgggt gggctttgac aacgaccgga
     3061 catcccgcag caagggccga agcagtgtct ccgcctgcaa cggagaaggc cagcggaaaa
     3121 ttgctcgccc cgaagacagc cactgggcca agcggctgaa gtaccgagcg gacatctggc
     3181 ttcggcagag gcttgcgttc cgggtccgca gtatcaatgc gagcattgac ccaagaacct
     3241 tcctctacca catctgcaaa aagacgaagc tggttggccg tgcgggcaag ctctccttca
     3301 agccggggaa gaggcaaagc agtttccaga tgtgcccgcc cgatgatttc agccgagact
     3361 ttctggagag cgtcggctat gctgcgcaga aatgccgcgc gcttgctgtt cgagagcctg
     3421 ctgtagctgg cgaaggctgc atcagcgagt tttgcagcga tatctatgtc ttctaacgag
     3481 gcggagaaga atgccggttg cagttgctca cctgtggcag ggttgatgcc gtaaaaaact
     3541 tctccggttt tggagccacg ctttggtccg aggatggatg ttccattcag cataggaaat
     3601 ggtcctgatg taatgatgcg gtctcccatc ctttcgttga aaaccgcgac aaaaggatgg
     3661 ggcacccgga ttcgtgtcgc gcaaagcgaa aaagcagaat gacaaatatt agagttggtt
     3721 taatgcgcag aggctgcggc aaagacaact tcttttatct caggccgttg cttcagcgct
     3781 tccgcgatgg tcctcttcgt ctcagctagc tcttcgcctg tgatttccag tcgaggcggt
     3841 cgcacacggg cgctgcccac tcccagttcc tgctgcacaa acttaatgag ttggataaac
     3901 ttcggaatgg tgtccatgcg cagaagcggc aggaaccagc ggtaaagctc gaaggcatct
     3961 ttggtcttgc cttcataagc gaaattgaag agcgctacgg attcagcggg catggcattc
     4021 accagtccgg caatccaacc gcaggccccg gcagcaatgc cttcgacaat cgcgtcatcc
     4081 acgccgacaa aaattttgag gcgatcgccc acaagtgcgc gaatcgcagt tacccggcgc
     4141 acgtccgtgc tcgattcctt tacggaatgg aagttgctgt gttcggcagc aagctcggca
     4201 atgtgctttg ggagaaaatc agtcccgtat gcgacgggat tgttgtacaa catacaggaa
     4261 agcggcgcgg ccttaaaaac agcggcgata tgcgccttca tctcgcgcca gtctccgcga
     4321 taaacatagg gtggcagcac catcaggcca gagcagcctg catcagcagc agccttcgcc
     4381 agcgagacgg cctctgcggt tgaaagcgct gagatggcag ctacaatcgg cgccttgccg
     4441 tttgcagtag cgaccatgtt tttcaggatg gcgatcttct catcaaaggt gagtgttgcc
     4501 gcttcaccaa gcgagccaag catcaccagt ccggtgcatc cgttgtcgag cagccattgt
     4561 gcatgctgct gcatgaaggc gtggtctact tttagttgtt cgtcgaaagc ggtagtcatt
     4621 gcgggcatta cgccaaacca gttcatcgtg tctcctgtaa ttccaaaagc tcgggtattg
     4681 cttcgaggca agaaagcgtt gctggaaaga ttggcgggcg cacagaaccg gcgttccatc
     4741 caaaaagaaa ttctgtggcc gcaccgcaga tgcgcccctg gcaaggtccc atgccgcagc
     4801 gcgtgtgcag cttggctgcg cgccacgatg gttcgtcttt taatgtgaga tacgaaacgt
     4861 cctcacagcg gcagatgaca gtatcatcct gcggcagttc tttcagggct gagtctagag
     4921 caaaagcctc ttccagcttc tgcgcaaact tgcgctgtcg tgcgcgttgt gcaaagagag
     4981 agcgtgcatt gtccggcaga cccgcggctg cataccctgc aatctgacct tccaccagcg
     5041 caagctccac gccgccaact cctgttggtt ctcctgcgca gtaaatgtcc agaatggagg
     5101 tttgctgcca ttcatctaca gcaactttgc catcgagaac acggcagccg agaagttcag
     5161 gcacctctgt attcggaaca agatggaagc cgcacgcaag gtaatcgcac gcaattgtcc
     5221 aggttcgatt gcctttgcgc agcgtgacgg attcgagtct ttccttgcct tgcgccgaca
     5281 ctggccagca gcccgtatga aagggggccg aaagtgtctg gcttttatac actacggcct
     5341 gcaagagctt ttctttatct gtaactatcg aagttgcaaa ttttagaaga cgcgagagcg
     5401 gggcctgttc gcagatggcc agtacctttg ccttgtgtct cttcagatac gcggctactg
     5461 ccagcagcaa cggaccgctc cctgcgacca caatgcgctt tccttctacg aaaagcccgt
     5521 tcttcaccag ggcctgcaat ccgccagcac cagcaacatt gggcagtgtc catcccggga
     5581 aggggaggaa gcgctcgcgt gctccggtgg cgagaatcag cttttcataa gcaacatcta
     5641 catagctgcc attctgttca gcacgaagga cacccggcgc aggctgcgca tatacacgcg
     5701 ctccctgaat acgcacaacg tttgcggcag ccacacgcgc gcgccatact tccgcggtct
     5761 tcagcggcgt atctttgccg cgccaaatct gtccgcccag cgtgaagttg tcatctacaa
     5821 ctgcgacatg ccttccgccc tcagcagcac aggccacggc agccattcct gctgggcctc
     5881 caccaactac gacaatgtcg aagcgctgta tcattgcgtg tttacttcca ttcccgcttc
     5941 gcagagcatc tggcaggtgc gttgatgagg tttgccgttc acgatggcgc ggcattcaaa
     6001 acagatgccc atgccgcaga gcggcccgcg tagctctcca gtggcggagg cgcggcaggc
     6061 tgcgccagca ttcatgatgg cggcggcaac cgttgtgcct tccagggctt cgcaccacct
     6121 gccattgatg gtgagtgtaa tgcgttcagg catagatcac ctcacgcgca aatcgctccg
     6181 gcaaataagg ccccagcggt atcgaagtgc cgcgctgcag cagatggtcc gttaacaaac
     6241 gcgctgttgc cagtgatgtc gtgatcccca gaccttcgtg tcctgttgcc aggaagaccg
     6301 atggatcttc tgtcggtcca atcagcggca gcttgtccgg agtggccgcg cgaaagcccg
     6361 tccacacccg aatcgaggac agctccttca tggacggtat gtaatcaaag gccctcgcca
     6421 gcatcgcgga gacaaccttc tggtctacgc cggcaccttc gttgtcatac tggcgcgagg
     6481 agccaatcag cagctgtccg gtgcgccgcg gctgcacatt aaaggcaaca gagtcgcttg
     6541 taatcgagtg cgcacttttc agatatccga gttccaccag ctgatggcga acaaaatccg
     6601 gatagcggtc ggtaatcacc aggtgccctt ttcgtttccg aatgggaaga tgaggcatca
     6661 gccgcgtcgc gcccgctcca gtagcgtgga tgatgagtgc ggcggaaagc tctgtgccat
     6721 cgctgaaagt tacacggcct ctttcagctt ttgccactgc atttcctatg tagagagcgg
     6781 ccccggcctg ttttgcgcac tccagcaagt acacagccgc gcatggagga taaagcacag
     6841 catccgaagg cacaagcagc gcgcctgcaa gcggacggcg caggttcggc tcagcttcag
     6901 agagcgcccg tgaatcgagg acctcagtcc ggacatttac agatgcatat accgcctgct
     6961 tgcgccggac ctcctgcatc tcttcatcat ctgcggcaat ccagagtgtg ccgcagcgat
     7021 catattccac atttgcgggc agttgaggag cgatctcgtt ccagagtgat tgcgaatact
     7081 gtgtcagagc aagctgggca ggtgagtcat ccatgacaac gatgtgcccc atgcctgcgg
     7141 ctgtcgctcc gccgccgaca atggatgctt ctacaatggc cgtgcgcatt ccggcgcggg
     7201 caagttcgca ggcgcacgct gcgcctacaa ttccagcgcc aatcaccacg gcatcatagg
     7261 tcgagatcat ggaacaatgc ccatgcggaa aggatcattt gcatccagaa ccagcgagga
     7321 ctctgctgtg acgtacgctt ctccggtaat actgggtagt accaatccat cgcgtacttc
     7381 aatcgagcct tcaaagactg tgtccagaat gccttcctgt cgccagatgt ctcccggcgc
     7441 aagtttgcca tcagcataga ggcaagccat ctttgcgctt gttcccgtgc cgcagggaga
     7501 acggtcgtaa gccttgcccg ggcagaggac gaaattgcgg ctatgtgcgt gtccgttgga
     7561 tggcggcccg aagagttcaa tatggtcaat ctccgcgcca tcatttccgg tgatgccgtt
     7621 tttgttcagc gattgccgga tggcccaggt gaagtccgtc agctcgccga catgctgcaa
     7681 gccaagcggc atcctgtggt cttctaccag ataaaaccag tttccgcccc atgcaatgtc
     7741 tcctgtgact ttgccgaagc cgggaacgtc cacggtcacg ccggattcgc ttcgataact
     7801 gggcacgttg gcgatggtca ccctaccgga tgaatgcagg gtcgcattca caacgcccac
     7861 cggcgtctcg atgcggtggg tgcccggccc aatcctgccg agccatgcca ggcttacaat
     7921 cacgccaatc gagccatgtc cgcacatgcc cagatagccg acattgttaa agaagatgac
     7981 gccaatgtcg catgaaggat catagggttc acagagcaga gcgcctacca gaacatcgga
     8041 accgcgtggc tcgttcacca cagctgagcg aaacgtgtca tgctgcgcgc gaaagcgctc
     8101 caaacgccca gacagagggc ctgaacccag atccggtcct ccggaaagca caagccgtgt
     8161 aggctcgccg gcagtatggg agtcaatgac ctgaacgcgg tgggatgtgt cgctggttag
     8221 catctaaaaa tgtctacaaa attggataca tcactgagta tatctgaatc ttttcccaat
     8281 gaaagcatat tcttttcaat gaggtattca ttaaattcgg catatccagt aacctgagct
     8341 tggctgctat gactcctcta aagaaaaccg tttccagatc gtccaaagcc gaacatggtt
     8401 ccagtctgcg gaccgccttt cacgaaattc gagagcttat cgtgcatgga aggctttctc
     8461 caggtacctg gattgtcgag ggagacctgg cagcacgtct cggcatgagc cgcactccgg
     8521 tgcgcggcgc actgcactgg ctgcagcgcg aggggtatgt tctggagcac aagaacgtaa
     8581 gcaagtcgcg catgattgtc gctccgctca ccaaagagga cgcaaacgaa ctgtactcta
     8641 ttatcggacg gatcgagggc cttgcgggac gccgcaccgc ttcgttacct caggcaaagc
     8701 gaacggaaat cgcccagcag ttgacccatt acaaccatca gttggaagaa atcgtgaaag
     8761 cgcgtcctgc aaagaccggc ggcatcttcg atctcgacca caattttcat cgcgccattg
     8821 ttgaagccgg agctggtccg cggctacttg cgcttcacaa agcgattgaa ccgcagacgg
     8881 agcgttactg gcggctctat gccagctcca tcattcatga cctccaccgt tc
//
LOCUS       DASRBV010000337        44506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_538918, whole genome shotgun sequence.
ACCESSION   DASRBV010000337 DASRBV010000000
VERSION     DASRBV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 44506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44506
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538918"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VFA02_12520"
     CDS             <1..1766
                     /locus_tag="VFA02_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZU10719.1"
                     /translation="WGSHAFLLLRSNLPKQLLCPTFMLNAAQAILNKILWPEQPSSAS
                     SSHLLPRWIFLRALGLIYFSAFYSLAFQIRGLIGPDGVLPAQKYLAAITRSFPGWEHI
                     WFAPSLFWLSSNNHMLMAITITGLIASLLVVINLWPRLTLLICFLCFLSFVTAAGDFS
                     NYQSDGMLLEAGFISLFFAPRGLWPGLGASSAPSLASMFLLQWEWFRIYFESGLVKLL
                     SGDPEWRNFTAMDEYYQNGPLPTWVGWYMQHLPHWFQAAATGATLVMELVIVFLLFFP
                     RRFRLICFCIVTPWEIIVILTANYTFLNYLVLSLGFLLLDDAYLLRVVPQRFRSAQLT
                     SILHADTHPVTLDEEQRGQSAEDTHRVAPDNTSLEGNEGPAGGKHHSYLTSIRRAVSA
                     VLLFWVFYATTAELIHMLWREAPIPFFPVAALEPFRIANQYGLFAVMTRGRYEIEFQG
                     SNDGQNWTAYPFRYKPQKLDDPPRIYAPYQPRFDWNLWFASLGSWQQNSMVPLTEERL
                     LQNSRDTLSLFAGNPFSQSPPKYVRAVLWQYWFTSMAERRATGLWWKRQLLGLYAPVL
                     TINPDGKFEAVQWPEPLPPHE"
     gene            1759..3222
                     /locus_tag="VFA02_12525"
     CDS             1759..3222
                     /locus_tag="VFA02_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU10720.1"
                     /translation="MNDDTSLLIGSKHKHPLHAVVIGSGPNGLSAAIVLAQAGWQVDV
                     YEAQPTIGGASRTLPLTLPGFQHDFGSAIHAMAAGSPFFSTLPLAKHGLEWVHAPAPL
                     AHPFDDGTAITLERDLDDAERVFGSDGKRWRSIFGPLAEHWNQIAKGALRPLVHFPAH
                     PFLMARFGTFSLMSAQFFADRFLQSSRVRALFAGIAAHSIMPLDAPLSASGGVMLAAA
                     AHAVGWPMPRGGAQSTANALAGYFSSLGGSIYTHSPITSLEDFSPHTVLVCDISPRQL
                     LDLASHRLTGQFQKDLRRFRYGPGAFKLDYALSAPIPWKAAECARAGTVHLGGTMEEI
                     AEAELAVWQNRHPDRPFVLLTQPTLFDPSRAPEGKHIAWAYCHVPNGSTFDMSERIEA
                     QIERFAPGFRDCILARHVSSPSFLETLDANLIGGDINGGAISLRQLVFRPTPRHYATS
                     DPNIFLCSASTPPLGGVHGMCGYHAAQAVIRHVRHRL"
     gene            3230..3370
                     /locus_tag="VFA02_12530"
     CDS             3230..3370
                     /locus_tag="VFA02_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10721.1"
                     /translation="MILTTEVDHHHVQFSLDGGKRRKNPDSASCATQHLLSGAVHRYF
                     ID"
     gene            3464..4378
                     /locus_tag="VFA02_12535"
     CDS             3464..4378
                     /locus_tag="VFA02_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU10722.1"
                     /translation="MDFEQLKTFLEVSRLKSFSRAADKLGVTQPAISAQIRSLENEVG
                     ARLFDRDGGKVTFTAAGRLFEPFAEHCLQASNHIMVAIGELHRSPRGEISISANEATS
                     LYVLPAVFAQFKKQYTRVNLSIVRADRSKSLEAVLNREVDFGVISLPVKDPRLLVEVV
                     HKDELALVVPKTHPLATREQIKFAEMLQYPLLLPKHGRQRDQIEDLFRTHDVNPRVGM
                     EVDSSELLKRLIGAGLGMGFLPRSNVRDDERAGLLKTLKVEGVRLNRELALVFRKDKT
                     LTRAAQAFLEIATGRSRPMLAAVGHKPR"
     gene            complement(4505..4750)
                     /gene="purS"
                     /locus_tag="VFA02_12540"
     CDS             complement(4505..4750)
                     /gene="purS"
                     /locus_tag="VFA02_12540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZU10723.1"
                     /translation="MKAHVYVTLKRTVLDPQGQTIQNALKKMQYKGIKDVRQGKYFVL
                     DLDGSLDAAAAKTEVERIAKDVLTNPVIEEFTYRLEE"
     gene            4829..5377
                     /locus_tag="VFA02_12545"
     CDS             4829..5377
                     /locus_tag="VFA02_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZU10724.1"
                     /translation="MVNYLELPIGDKVPEVINSVIEIPFEGINKYEYDKQLHVFRLDR
                     NLYSPVHYPGDYGFIPSTLSDDGDPLDVLVLVDAPSFPGCVMQVRPIGLLEMMDQGVL
                     DEKVLAVGKNNPRYKDVWNYSEIYPHMLKEITHFFSIYKDLEGKRVEIRGWHDASYAR
                     DRVLRAAENFANNKKQVEVAKR"
     gene            complement(5449..7920)
                     /locus_tag="VFA02_12550"
     CDS             complement(5449..7920)
                     /locus_tag="VFA02_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZU10725.1"
                     /translation="MSETPRRPKTKLRIFLAAVVLLALAVVLPPLINLNRYQGEIAAA
                     ISRAIGRPVHLSSVTLRLLPRPGLELGDFVVEEDPAFGAEPALRAPSVDASIRLSSLW
                     RGRLEIGRISLDQASVNIVRDASGHWNLGTILVQAAHIPNAPTAQRHAGQSPRFPYIE
                     ASNTRVNFKIGAEKKPFSFFNADFAMWLENPDEWRIRLEAQPARTDIDLDLADTGTLR
                     VEGSMKRASELGDMPVSLHAEWTNAALGQISRLLLGAESGWRGNLEITGDITGSVYGP
                     QFKTRIRITDMHRQEFSPVEPFNVDATCQGGYRQTERSLRALTCFWPISDGHLLLTGN
                     VPDIEHPVPALHLHVQNVPAAFGLSALRLFRNKFADSVQISGTAQGDLDYAHQLTGDV
                     AVEHLSLRLPDMDSPLAVPTLHIVARQTAPAPRKGKKSQPKPASPAALQLENADIPLG
                     GASPLELSGNFTRRGFSLNFQGDASMERLRPIASGLHLASGIVSALAPKGEAELDLKV
                     HGPWISPVSTSDYPVPATTTEGVLHLKNAEYQAGFLSGPVEIVSAQVSLSPTQVVVSP
                     LAAVFQKIPVSLSVTIPTRCSGENCAPEFSLTAQNLDAASLQSALLGAGEHNELLQQI
                     LSRLERNKPQWPALNGTIHISTFMLGPLAVHDASGRLHIEGRAVQFLTLDGHAWNGVL
                     HATGRMDASGTPKYSFDTQLTHANAAEIASMWHGERASGAIGLRAHIEAAGYSADDLQ
                     HSAQGTFQWDWTQGSLAAAPAALEHFDHWSASGVIRNAQLVLEKSQVSRGASRSDVSG
                     EITFDRKLNLSVSEKTEQAHAAHAP"
     gene            8268..8564
                     /locus_tag="VFA02_12555"
     CDS             8268..8564
                     /locus_tag="VFA02_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZU10726.1"
                     /translation="MPVAAMTIEIRIEHAQSLKDRRQVVRSLKEKLRNGFNVSVAETD
                     KAVTWQSATISVAAVSSSRDYLSGLMQQVEDAVVRITNDLGAQVTDAWWEFLEN"
     gene            complement(8618..9619)
                     /locus_tag="VFA02_12560"
     CDS             complement(8618..9619)
                     /locus_tag="VFA02_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU10727.1"
                     /translation="MAALAQAPTPPPIPPLQYADLGNLKLENGSVIHDCKIGYRTLGT
                     LNAAKSNAVLFPSWFTGRSSDIAIQVLSGHYIDSTKYFVIAVDALGDGVSCSPSNSTT
                     QHGTDFPQFSIIDMVNAEYRLVTETLHLQHLRAVLGVSMGGMQAFQWMVSYSDFMDAV
                     IPVVGTPQQTSYDLLLWNTELKALEADPGYNGGKYTQPQPLPIVALIHNMNLATPIFR
                     ANHTTREDFPQYFEQFTKEGDRFFDANDYLRQLQAMIGQDIAHGGSLYDAAKKVRAKV
                     LIISSMQDHMVNPLPALGFAKLIHAQTLVLQSDCGHMAPGCEIGSVTAEIAKFLEQL"
     gene            9794..11758
                     /locus_tag="VFA02_12565"
     CDS             9794..11758
                     /locus_tag="VFA02_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HZU10728.1"
                     /translation="MNTLMPEPAKLTAEQGTLAITPSFSVAAPHFRDARLDGAIGRML
                     HRLEYATGVPVAKDIHKDASGASFVIDVRGPGETIQSVDEDESYSLDVTPSGATLKAN
                     TVVGALRGMETLLQLVQAQQGKYVLPAVSIQDSPRFQWRGLLIDCGRHFEPVDVIKRT
                     LDGMAAVKLNVFHWHLTEDQGFRIESKVYPRLTEMGSDGLYYTQEQAKEIIEYARERG
                     IRVVPEFDMPGHSTSWFVGYPEISSGPGPYQIRREFGISDYAMDPTRDSTYKFLDKFI
                     GEMAKLFPDPYMHIGGDETNGVQWKSNPKIVAFMQQHNMKDTAALQAYFNQRLLKILQ
                     KHHKHMVGWDEILNPALPKDVVVQSWRGVESLSAGAKQGYQGILSAPYYLDAMKPAGE
                     HYLADPLPSSSDLTPEQRKLVLGGEVCMWGEHVNERTIDSRIWPRTAAIAERLWSPEN
                     VRDVEDMYRRLDVMSVRLEAFGLTHLTQEDVGLRALAGTTDIHALRFFASVIEPVSFG
                     ERYQAQHTSQLTPLDRLIDAVRPDPPAKHRIAFIVQAFLKDPAGHQDEKREITLFFRG
                     LQVLAPKIQEQVATSPLLAEDATLRAQQLPELAQIAQDAVRYLTGETAPAGWKQSSLA
                     AMDATQKPSGMVRFVFLDPLRQLVNAVPEK"
     gene            complement(11873..12835)
                     /locus_tag="VFA02_12570"
     CDS             complement(11873..12835)
                     /locus_tag="VFA02_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10729.1"
                     /translation="MSSALQLEILDLRHFSASSLRPVLEEESRVWSERLHWDYRSSAD
                     LLLQYLDSRILPGYVAVENGRIAGYAFCVYEERKAIIGDVFTLPHVSVSVEEELLRHL
                     IELLQHSPGIERIECQLLLHTHGLHEEIFQRAGFKLFRRLFMELDLSHYTPPAVSALP
                     EGIAVYPWRESDYHPSGYLIAEAYEGHLDSYINDQYRTVSGSLRFLHNIVRFPGCGLF
                     DPLSSRTVVRTKDGALAGVLLCSRVREDVGHITQICVSRSLRGKGLGQRLIEACAENL
                     RARNFTGLTLTVTEENANAVELYRKIGFQERHSFDAMVWDRRHS"
     gene            complement(12974..14716)
                     /locus_tag="VFA02_12575"
     CDS             complement(12974..14716)
                     /locus_tag="VFA02_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU10730.1"
                     /translation="MPIPKSIKTLRTAPTTLLGRFPFLGTALETLTLFLFTAFFLFYG
                     LVPIFGGDGIGLVGADEPRYAQIAREMLARHDYITPILYGKPWLEKPALYYWRAMFAF
                     REFGVHDWSARLPSASFAFVLVVLIYLHMRRFRPGGQLDAALLTASCAGILSFARGAS
                     TDMQLAAPFCIGMLGWYAWYETDSKFWLFDLYFFVGAATLAKGPVAPFLAIVIITVFA
                     ALRKEWSIIRRSIWWPGIGLYFAMVLPWFIAVQKRNPNFLRVFFLEHNIERFATNRFE
                     HEQHFWYYIPVILLSVMPWTVIAIAALTDAIKDSVAEWKARRAKMHYMGHPRAGDAFP
                     EFLVLWALIPIVFFTFSESKLPGYILPSVPPLTILCGDYLNRMRTKGLKPWLLVLHGV
                     IVGCLTMLVLLLPRLIQHTDQLPPGNALFAAGTTGFAATIFILITTARFGLKRLRIAT
                     MVPIVILVLFLYGIGPFLGFGEVKSTKGTVQLIDYTYSARPLAKMLEQIVSSDGTVAV
                     YKVRRDMEYGLAFYRNKKTVNYDTDGIPPEQHILVVRASYVNDLRQKLEGRRYEPVFV
                     YPAQNLVVYMVAAQ"
     gene            complement(14774..15751)
                     /locus_tag="VFA02_12580"
     CDS             complement(14774..15751)
                     /locus_tag="VFA02_12580"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZU10731.1"
                     /translation="MAERHNIHAVLDAIERGQKFLVSAHARPDGDAIGSMLACATILE
                     QLGKRVEMVSCDRVPLIYRFLPCSSAIRQVSRVDSDYDAVILLECDGIERSRLHGLEG
                     RFLINIDHHVSGKAFANINWIDTEACAVAEMIYELAIVAGAHITPEMATCLYTAVLTD
                     TGQFCYDGTDAHTFELAAQLVRHGANSAAIARDVYFSRPTSKMLLLGAALSNLRREGR
                     IAWMWVTHEDMLRTNAAEEDCEGLVNYAIAIAGVDVAIFLRELPNHRVRLSLRSKGKV
                     NVAHIAEAFGGGGHPHASGCTLDGPLPDAAGMILAVAKKMLGRVVHEVA"
     gene            complement(15820..16194)
                     /gene="rbfA"
                     /locus_tag="VFA02_12585"
     CDS             complement(15820..16194)
                     /gene="rbfA"
                     /locus_tag="VFA02_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZU10732.1"
                     /translation="MPEQRARKYHQDRVAEALREEIGAMIEGELSDPRISFAYVSQVV
                     LNPGGKSALIYVAVDGGPKEEEDTLEGLMAARGYIRHELLERMGVRHVPDLVFHIDRS
                     EKMKARIDELLGRVKKRSRTPG"
     gene            complement(16389..19826)
                     /locus_tag="VFA02_12590"
     CDS             complement(16389..19826)
                     /locus_tag="VFA02_12590"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10733.1"
                     /translation="MAQTTISTGSIAGTVTDPSGAVVSDAKVVITNTGTGQALSLTTN
                     SSGAYNSGAIAPGNYKVEISAPGFSTMAQAVTVQVGNTSTVNAKLAVGQGTTTVEVQG
                     SAVTVNTEQAQVQGVLNAQQIDNLPVNGRNFLDLAQLEPGVQIQDGQNFDPTKAGYSS
                     ISFGGRFGRTARINVDGVDVSDETVGTTTEDIPSSAIQEFQLAQSSLDMSTDLTSSGA
                     VNVTTKSGTNRLHGQAFGLFRDASVGGASSPGGTNLPFQRSQFGGNLGGPVLKDKLFF
                     FGDAERIKQDSFAPVQFASPFNSYNGGFSVPFRETEVLGRMDYNVGNSGKLFYRFSYF
                     ANSLNSDFGLGYAVYDNKDYTRQHVVGYDFVAGNYSHSIRFSYLKFQNQIVDRVKGSS
                     LPFANTGVTLASVTGGFYSGPSPLAPQSTPQANTEVKYDGSRLLGAHLLRYGVMWNHI
                     QGGGFANFYGTAPRIRWQVNSATEAFAAAHGGANNPLNYPVYDVRVGNGLGFSTLEPA
                     LGFPAGGLGPDNRIGIYFGDVWKFSPNSTITLALRYDRDTGRTDSDLGPIPEVNAAFP
                     GFGNRVNLPNTNFSPQMGFAWDPFRNGKTSVRGGIGLYYENVIYNNVLFDRPLRLRTG
                     AFNQVSPACNAGTALPVPTNAGLITIDPSVCTSTVGQAIPGVLAFWQQVLAGNPLDLN
                     APNPNFIGGSSWLGAGAGIPLGFFAPKYKTPRSIQINIGVQRELRQGMVLSVDYLRNV
                     EEHTLLGIDENHVGDIGNFNLANAQAAIAATLAACGVTSIDQAIAACPGLYDQSNGAV
                     GASMSDFASHGLTADTDFGQACIQAIGVPCAFGGRNPGQAAAIFLEPRGRSIYNAMQV
                     KLVQNMKRMNFQFSYSLSRLENSGGAQASGTAGDSDQDFVIGAADYNDPNRYFGPNLL
                     DRTHQISLGGFGDLPWKFNLGLIAHFYSPLSSALVIPNFGLGPGEIFRSDFTGDGTTQ
                     DLMPGTHMGNFDRGIDAKKLTQVINNYNAKYGGQPTPAGQVLVQNGLMTVAQLQRLGT
                     NSSSATPDGVAPVLSTPPSDQANFTWLRALDLNVGWKLTIKERFTIQPSVAFFNLLNF
                     GNFNLPPNTMSGLLSGSPGAINGTGKLDNETFRVGNGTGVYALGAARQTEWGLKFTF"
     gene            complement(20157..20663)
                     /locus_tag="VFA02_12595"
     CDS             complement(20157..20663)
                     /locus_tag="VFA02_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10734.1"
                     /translation="MQLPQVFFVIFAIAVAVGVLLQAGVLLGMFFAIKKSVRQAKEVS
                     DKINQHLVPALATARNLLEDVSPKIKVATANLVETSHIVREQAHRVERTVNEVTTAAN
                     AQVQRVDEMATAVLDGVTQATAAVQHGITTPLRHVAGVLNGLRAGFDVLRKKETPLHV
                     EEDGGNFV"
     gene            complement(20716..21111)
                     /locus_tag="VFA02_12600"
     CDS             complement(20716..21111)
                     /locus_tag="VFA02_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZU10735.1"
                     /translation="MSDEKSNGFSWFLAGLGLGALLGVLYAPKSGRETREDLVNSARE
                     GGEYIRQRSREAADQVNAYVERGKAQVGEYVDRSKEYFEKGRAQWNDLISQGRQFVSE
                     QGERVSAAVEAGRQAYRSTTEAGQEAANQ"
     gene            21323..22426
                     /locus_tag="VFA02_12605"
     CDS             21323..22426
                     /locus_tag="VFA02_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZU10736.1"
                     /translation="MKHAYTIKTRGIQAALLATALVLGAGCNRQQQEQPQARTDQQVA
                     SDIQAKIAAESALNGQQIQVSVSNGVATLSGSANDEASRALAANDAGSVNGVKTVINN
                     LTVVPLPQPTQQAAAPAPAPERKTSAERKKKRSGQEVAETAPAPAPAPAPAPAPQSQA
                     QPAPPPPPPQPVVKTITIPAGIIVPVRITEGLSSQTAQPNQVFHGSLAGDLIADGMVA
                     IPHGASVVGRVVDAKDATHFSGNSLLSIELTQISAHGRQIPVVTDAYSRQGQGRGKNT
                     AMKAGGGAALGAIIGALAGGGRGAAIGAAAGGATGAGVNAITRGQQVQIQPETLINFK
                     LQSPITVTTSQKAGEVRSFDDNSNPQLQNRPQQ"
     gene            22552..24882
                     /locus_tag="VFA02_12610"
     CDS             22552..24882
                     /locus_tag="VFA02_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HZU10737.1"
                     /translation="MNRRRFLQGLAIGAAGMSAWGDTQNEHSPVVATTLVSPIDLGDQ
                     TLLCSFEHEGQSWKVYEDLRVRDGAFTFVRSDGQGKQLRKTAEATFAENGPPYLGIPL
                     KDIGLSARDLLADRLLEHGDPDPDQVRRAAPPLESAPPERGGYRLPWNTFVGTRECSD
                     TMPVFPSGNTRTYHPNQFFPDLKPGLAQRRFEGLIGGWMPAVRKVMPLSGSAYYELII
                     FGDVLARDRFIVQTWHRTALIENGKITKVEYGYSYPPFPPRRHAPQPEEFYRGLLLFA
                     AYWEDLLRDIASAHLPDATYSDMTRHAFARELMVRPGGVYPKYGAVDRDYYGSEYDGF
                     QDIFTSSVFANLDWGRFEMARQVIDNYLTRFTDDSGMINMRGPETAQFGLTLFLLARY
                     YQYTGDAALLLKHRKKIEATASLLLELHDESLRLSPSDPGHGLIHGWSESDSCLFPKP
                     DVWWQPYFSNSAFAARGLSEISSVWNSLDAPGMKALASGWRQRSDTLRRRVIESVEQS
                     VRRDMAPPYVSPLPGTKLTFRESMEQEHPSPQGWPHRAYSELLQAEVLPQQLANAVID
                     CMRAYGATTLGVVANVSRPRETGRDILGFISYGYARALLALDRIEEYLLFLYSHRYHD
                     HTPGSWVAGEVAGITGDSALFCIPAQLTIPLLVRWMLVFEDADEDRLYLGRALPRSWV
                     GSEKEISIEHAPTRWGRVSFSLKTNMTAKTAHASVTLEKQGAHEIYLRMRLPEGAHLL
                     SARVNGQPANLAGKQKDTILIQAGNERTFEVEALLA"
     gene            complement(24939..26657)
                     /locus_tag="VFA02_12615"
     CDS             complement(24939..26657)
                     /locus_tag="VFA02_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HZU10738.1"
                     /translation="MKTGRPQGYMTFTLHAHLPYVVHHGTWPHGLEWLLEAAAETYLP
                     LLRVFRRLEADGLALKCNINITPILLEQLAHPTFKKEFPQYLKRKILAACEDESYFRQ
                     NGEKHFAQTARYWLDFFEQDLKDFEELGGDIIKGFRHFNDSGQIEVITCAATHGYFPL
                     LGTDESITAQVKTAAATHERYLGKKPRGIWAPECGYRPAGRWQAPVAPAGGALWPPFE
                     RIGVEEALAEGGIEYFFVDSHLVEGPVKFTPYELKAGGPVGVAAATEIVENNGHGFYQ
                     PYLVDGPRAHQHPVAIFPRDPKTGVQVWSGEMGYPGDSNYLDFHKKRWPGGHRYWQVT
                     HPKVDLGAKTPYYPERAAERTADHARHFVSVVYDALKDSFGNGRTPILTSPFDAELFG
                     HWWYEGPLWLEHVARIMAKEEIPIALTTCGDYLEKEPPSGYLALAEGSWGKNGTNEVW
                     LNPDTAWTWTHIYPAETRVRELATEGRWRDGGTGERIMKQLCRELLLLESSDWQFLIT
                     TAAARDYAEMRFQTHVNQFKTVEKAWDEYAATGGLKPETEKHLKEIEDRDNVFHDVDP
                     GLWKKR"
     gene            26779..28035
                     /gene="glp"
                     /locus_tag="VFA02_12620"
     CDS             26779..28035
                     /gene="glp"
                     /locus_tag="VFA02_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZU10739.1"
                     /translation="MERMNPASKQATPDHVVSFAEAVRTVRQEAEKIREVRPAAEQVP
                     LLDATGRVLAEAVRADRDQPPFPRSTRDGFACIAADVQSGRMLRIIGQVRAGEAWQGG
                     PLQPGEAIEIMTGAPVPDGANCVVMVEHVQAEENQLRLKAGHSIAPGENIVPAGAEAK
                     SGTVLVPAGTRLVPSHIAAAAACGYAQLSVFRKPRIAILATGDELVSLESTPLLHQIR
                     NSNSYSLAAQIQLAGAEPVILPVAHDSQPAIEAAICGAQDCDLLLLSGGVSMGKYDFV
                     EQVLHHRGAEFFFTGAKIQPGRPVVFGRLPHQYFFGLPGNPVSTMVTFALFAHPLIAA
                     LSGEASGGSRFALAGLTHEAHCKSGLTRFLPARLDYSIHGATATVIPWQGSGDLASTA
                     NANGFAVLPAEDKRLPAGSLISVLLM"
     gene            complement(28231..29478)
                     /locus_tag="VFA02_12625"
     CDS             complement(28231..29478)
                     /locus_tag="VFA02_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HZU10740.1"
                     /translation="MLRRWRTRILLFLAVLGPGFITANVDNDPNGILTYSQAGAQFGY
                     SLLWTIIPITLALIIVQEMCARMGVVTGKGLSDLIREEFGLRMTFIIMVLLVIVNFGN
                     IIGEFAGIAGSLQLFHLSKYISVPICAALVWLLAVRGDYKSVEKIFLTASVFYIAYII
                     TGVLSGPSWREAMVATVKLPSRAAWAQHDYIYMVVGVIGTTIAPWMQFYLQSSIVEKG
                     VSVKNYAATRLDVIVGSIFTDVVAWFIIVACAATLWAHGMGSIAMPADAAEAMKPLAG
                     PYAFLLFAFGLFNASFFAASVLPLSTAYTVCEGLGFESGVDKGFRQAPFFYWLYTLLI
                     AGGTAVVLIPNFPLVQFSIFSQTLNGVLLPIVIVFMLLLINRKDLMGTHVNARWFNVA
                     AWLTAITVSVLSLIYMVQLVRHA"
     gene            29625..29996
                     /locus_tag="VFA02_12630"
     CDS             29625..29996
                     /locus_tag="VFA02_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10741.1"
                     /translation="MNDRPALSATLSSMPQFKSGSTGAIALSLIIAVASAWMGIGANG
                     IYCDRLPSMTIMATLAAFVIALCFRLFARADTRAKKAQLAMALIIAAGTLFADTRFVL
                     KYRGICGQLQQQIRQMRNPSQ"
     gene            complement(30046..30741)
                     /locus_tag="VFA02_12635"
     CDS             complement(30046..30741)
                     /locus_tag="VFA02_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZU10742.1"
                     /translation="MNKVVSSADEAVRDIPDGSTLMIGGFGLCGIPENLISALVRRGV
                     KGLHTISNNMGVDGFGMGLMLEAGMIASHTGSYVGENKLLEEKVLKGELDLKLVPQGT
                     LAERIRAGGAGIPAFYTPAGVGTLVAEGKETREFDGRPYLLERWLRADFALIKAWKGD
                     TAGNLIYRKTARNFNPMMATAAKFTIAEVEELVKPGELDPDHIVTPGIYVKRILKGEK
                     YEKRIERRTVRKA"
     gene            30918..31319
                     /gene="mscL"
                     /locus_tag="VFA02_12640"
     CDS             30918..31319
                     /gene="mscL"
                     /locus_tag="VFA02_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZU10743.1"
                     /translation="MLKGFRDFILRGNVVDLAVAVVIGAAFTQVVNSLVKNIINPVIA
                     AVAGKPDFSAFVLDVHGGKITYGSFFNDVISFLLISAAVYFLFVLPVNKLMSKFFPAK
                     AEPPKTKSCPECLSDIPVAAKRCAHCAQPVV"
     gene            31477..32211
                     /gene="cobA"
                     /locus_tag="VFA02_12645"
     CDS             31477..32211
                     /gene="cobA"
                     /locus_tag="VFA02_12645"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZU10744.1"
                     /translation="MKHAEKGKVYLVGAGPGDPELLTLRAASLMQTADCIFHDDLVAP
                     EILTLAGASALVENVGKRCGNKTITQEQINHLLVEKAKAGNSVVRLKTGDPLLFGRGG
                     EEIEALHKDSVPFEIVPGITAGFAAAAALEASLTDRRLASKVIFLTAHRAQSGEEASF
                     GPLPEDATLVIYMPGSDYARLARQLGDARVAPETPCMIVSGASTPQEQILHTSVGDLA
                     QTAKLPAPCVVLAGKALSIHRIKKTG"
     gene            complement(32251..33027)
                     /locus_tag="VFA02_12650"
     CDS             complement(32251..33027)
                     /locus_tag="VFA02_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZU10745.1"
                     /translation="MTHTGKMTLQAVSALGALSLLLATGCTTKNYVRSQTTPVIQKTN
                     ELDDAVATNNRNLHDVDQRAQAGITQAQNSANQAQQSAQSAGTAASQAQQSAQEAVNR
                     VDSLSGVVANLDNYKQVADVQVNFGFDKADLTRSDKQKLDEFAAQLNGQKSYILALTG
                     GTDSTGPADYNYALSQRRAEAVVQYLASKYNVPPHRFYLVGIGKDKAVASNSTPSGRA
                     KNRRVEIQLLSNMSGSDNQTAQSTGTVKLPQEGQVTSPQQ"
     gene            33269..35161
                     /gene="uvrC"
                     /locus_tag="VFA02_12655"
     CDS             33269..35161
                     /gene="uvrC"
                     /locus_tag="VFA02_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZU10746.1"
                     /translation="MDLQEKIRTLPARPGVYLYKNADGEIIYVGKAKDLRSRVRSYLL
                     EASQANAKTGSLMREAVDLDYILVDNEHEALALENNLIKQRKPRFNILLRDDKTYPYV
                     KLTLADRYPKVFVTRRLRKDGSAYYGPYFPGNLAHRIVDLIHRSFLIPSCKVDLSRYH
                     PRPCLQYYIKRCLGPCVEGLTTPEAYREAVRDTQLFLEGRAAELGKSIEDRMHTAAAN
                     EQYELAAKYRDLLRTVSELQEKQRIASAENDDADVFGYHFENGMLAVNLFHMRVGKIV
                     DRREFFWEDLPELLAQDGDEEQIAKQSANFQAGTFFSALLKQLYIDQQYVPRSIFVPV
                     DFTDRETLSALLSQQTGHKIEIAVPQRGEKRSLVDLAGQNAKQGYDQRFRVMQPSQKA
                     IQEALQDVLVLPELPRRIECFDISHIQGAETVASMVVWEDGAMKKSDYRKFQIKTVSG
                     VDDFAAMREVVTRRYKRVAEENKPMPSLILIDGGLGQLHAAASALEELGLTSQPLASI
                     AKREEIIYLYGQEDDPIVLDRRSPVLHLVQRIRDESHRFAIAYHRKRREMRDRDSELL
                     TIPGVGARTRTRLLEHFGSLRSVQNAGIEALTAVVPRRTAEQIYAHFHPHPASVIVSL
                     PADGHS"
     gene            35280..35870
                     /locus_tag="VFA02_12660"
     CDS             35280..35870
                     /locus_tag="VFA02_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU10747.1"
                     /translation="MFGNFTHEGGRCGHWKHFRGGRGWFGEAGEFWGRRGHRFGGDFG
                     GRLFDHGELRYVILQMIAEKPGYGYEIMKVIGERLAGIYAPSAGVVYPTLTLLEEEGL
                     ATVTSTEGGKKLYAITEAGKKELKANKALVDAIFYRMDKVGAAFGRGRSPQIMRAMHN
                     FRLALKLRFAQGDLSKEQINKIAEIIDAAAKQIESI"
     gene            35857..36015
                     /locus_tag="VFA02_12665"
     CDS             35857..36015
                     /locus_tag="VFA02_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10748.1"
                     /translation="MSPSEEDTLLERLYELEAKNLQLQTLVVELLDKNQRLRNSLARY
                     EQQVEVQN"
     gene            36057..36932
                     /locus_tag="VFA02_12670"
     CDS             36057..36932
                     /locus_tag="VFA02_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZU10749.1"
                     /translation="MREFVYHLCDVFTGTPFQGNQLAVFENAGGLTTAEMQKLANETN
                     LSETTFIMRRSAEMEANRGVRVRIFTTQEELPFAGHPTLGTASFIRRFLPEFSERELI
                     TLELNVGAVQVRFTSPRHGEMTQPNPVFGQMHEREAVAQAIGVSTDDIASDMPVQTVS
                     TGMPFCIVPFRSVDALSHLRIPGALAEAYLKNTDAKFFYCITQELQGVWRARMQFYNG
                     EDPATGSAAGCAIAYLVRHGFAASGEQLHIRQGIEIGRAGEIGVRAKFDGVSVSEVRV
                     SGSTIFVANGRFFLE"
     gene            37486..37914
                     /locus_tag="VFA02_12675"
     CDS             37486..37914
                     /locus_tag="VFA02_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU10750.1"
                     /translation="MRPRKVILCVDDNEQALSVRKFMLETRGYRVVTALSAEEALEVF
                     RSGGVDLVLSDLIMPQMDGNEMVRRMKDIAPDVPMILLSGTVKAFDRASHADAFLPKG
                     ACTPLEMLERIRIMIARKRGPRKATPRPFYQPQTAMPSIA"
     gene            complement(38072..39667)
                     /locus_tag="VFA02_12680"
     CDS             complement(38072..39667)
                     /locus_tag="VFA02_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HZU10751.1"
                     /translation="MQKQRRDFLKYATVGAAGAFAPPGIGAQPAASAPPAHGSIYDVR
                     AFGATGDGKTIDSPAINKAIEAAASAGGGTVLFPAGTYNSFSIRLKSNVALYISQGAT
                     ILAAETPSSGNEGYDPAESNAPWENYQDYGHNHWHNSLIWGEGLHDVTICGPGLIYGK
                     GLSRGLSHEEPRAEEPRVGNKAIALKNCRNVILRDFSILKGGHFGILATGVDNFTIDN
                     LKIDTNRDGMDIDCCKNVRVSNCSVNSPWDDGICPKSSYALGYARATENVTITNCYVT
                     GTYELGTLLDGTFKKFPPGQRIPHTGRIKCGTESNGGFKNITISNCVFEGCGGFALES
                     VDGALLEDIAITNITMRDIMNTPIFVRLGSRMRGPQGVPVGDARRILFSNIVSYNSAS
                     HQAVLVSGIPGHAVEDLKFSNIYLHHQGGGTAEMAAIQVPEKEDAYPDPHMFGPLPAH
                     GFYLRHVNNVELTNVEIAYTAPDARPAFVLEDVHEADFFRIRLPKDSHAPIFRLKDVS
                     DFRIFGCAKIRDKSVESTQSESF"
     gene            39796..42054
                     /locus_tag="VFA02_12685"
     CDS             39796..42054
                     /locus_tag="VFA02_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZU10752.1"
                     /translation="MRLRILFAGLCFATCLYGQVTVADYERADHLRDKYKGLALNIPE
                     EPHWIEDTDSFWYRKTVEGGHAFVLVDANALAKRPAFDHEKLASALSSASGEKYTAVT
                     LPFQRFEFVDKQTAIQFRIDRQRWRCDLAKYVCEKAGAPEPEDDDGGYDDTPKAVNSE
                     TSTIASPDGKFEAFIENYNVYVRAKGKPEKTALSWDGSEDNYYALSTIVWSPDSRHLV
                     AYRIRPGYKREVHYIESSPSDQLQPKYSSMVYPKAGDVLALPQPVLFDVQEKRAIHID
                     NALFPNPYDLSRAEWWKDSRGFTFEYNQRGHQVYRVIEVNAATGAARVLIDETSKTFI
                     DYRPLVPDQYDTGKRVRIDVHDGKEIIWASERDGWEHLYLYDGATGKVKNQITKGDWV
                     VRAIDHVDPEKRVIWFEASGMEPGKDPYYMHPYRINFDGTGLTPLSNQDADHRVVFSP
                     DGKYYAVTWSRIDLAPVMELRRADDNKTLMTVEQGDIHKLLDAGWRPPEVFTAKGRDG
                     KTDIWGVIYKPANLDPQKKYPVVEAIYAGPQGSFVPKSFGPWAQPLTELGFVVVQIDG
                     MGTNNRSKAFHDVAWQNLKDAGFPDRILWHKAVAAKYPWYDISRVGIFGTSAGGQSAM
                     GALLFHPEFYKVAVANSGCYDNRMDKIWWNEQWMGWPVGPQYAASSDVDNAWRLKGKL
                     LLIVGEMDKNVDPSSTFQVVNALIKAKKTFSLLDVPGGGHGAGGEYGQRVLEDFFVHN
                     LLNQEPPDWNAQ"
     gene            complement(42186..42626)
                     /locus_tag="VFA02_12690"
     CDS             complement(42186..42626)
                     /locus_tag="VFA02_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU10753.1"
                     /translation="MLVLIVLLISWLVFRAMGAAGVHAFASWQESARYALIVMFIFTA
                     TAHFNKMKHDLARMIPSYFPHPLLIVYITGVLELLGAVGLGLPSFRRLAAICLIALLA
                     GMFVANVNAAKQGITLRGKPPTPLWLRIPMQLLFIALLWWSTHA"
     gene            complement(42896..44494)
                     /gene="pruA"
                     /locus_tag="VFA02_12695"
     CDS             complement(42896..44494)
                     /gene="pruA"
                     /locus_tag="VFA02_12695"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZU10754.1"
                     /translation="MATAEISTVILPRSPQGEFRNEPFTDFSKHENAQAMKEALVYVG
                     DRLGHEYELIIGGERIKTPGKIESRNPARPSQIVGIHQKAGAEHAELAMQAALKAFET
                     WRFVSAEERASLLLNAAAIIRKRKFEFCAWLTYEVGKNWAEADADVGETIDFLEFYAR
                     EALRLSKATTPIQYPGERNELLYIPLGVGAVIPPWNFPFAIMAGMTSAAIVTGNTVVL
                     KPSSDSPTIAERFVDVLEEAGMPAGVVNFCPGSGATFGNAIVEHPKTRFIAFTGSKEV
                     GLDIHERAAKTKPGQIWIKRTILEMGGKDSIIVSADADLDSAVEGVVASAFGFSGQKC
                     SACSRAIVEEPIYDVFVDRVRERVAKLTVGDPALNPNMGPVINAGAMQSILEYIEIGK
                     KEGRLIAGGNAIETPEKGHFIEPTVFADIAPNARISQEEIFGPVMAIIKARDYDHALE
                     IANNTEYGLTGAVYTPDREKLNKARRLFHVGNLYFNRKCTGAMVGAHPFGGFNMSGTD
                     SKAGGPDYLYLFTQAKSVAEKLQR"
BASE COUNT         9954 a        12961 c        11939 g         9652 t
ORIGIN      
        1 gatggggttc ccatgctttt cttctattgc gcagcaacct gccaaagcag ctattatgtc
       61 ctacattcat gttgaatgca gcccaggcaa ttctgaacaa aattttgtgg ccggagcagc
      121 cttcttccgc cagctccagc cacctgctgc cgcgctggat ttttctccgc gctctgggcc
      181 tgatctattt ctcagcattt tattcgctgg ccttccagat tcgcggcctt atcggcccgg
      241 atggcgttct gcccgcgcaa aaatatcttg ctgcgattac gcgttctttc cctggatggg
      301 agcacatctg gtttgcgcct tcgctttttt ggctttcaag caacaaccat atgctgatgg
      361 ccattaccat aactggactc atcgcatcgc tgcttgtagt catcaatctg tggccgcggc
      421 ttacgctgct catctgcttt ttgtgctttt tgtcttttgt aacggccgcc ggcgatttct
      481 caaactatca atcagacgga atgcttctgg aagccggatt catctcactc ttcttcgccc
      541 cgcgcggtct ctggcccgga ctgggagcat ccagcgcgcc ttccttggcc agcatgtttc
      601 tgctgcaatg ggagtggttc cgcatctact ttgaatccgg cttagtgaag ctgctgagcg
      661 gtgaccccga atggaggaac ttcactgcca tggacgagta ctaccagaac ggcccgctgc
      721 ctacatgggt cggatggtac atgcagcacc ttccgcactg gtttcaggca gcggcaacag
      781 gagcgacgct cgtcatggaa ctggtaattg tcttcctgct gtttttcccg cgccgcttcc
      841 gcctcatctg cttctgcatc gtgaccccgt gggagatcat cgtcatcctc acggccaact
      901 acactttcct gaattatctg gtgctctcac tcggcttcct gctgctcgat gatgcatacc
      961 tgctgcgagt tgtgccacag cgtttccgtt cagcccagct cacttctatt cttcatgctg
     1021 atactcatcc cgtcaccctg gacgaagagc agcggggtca aagcgcggag gacacgcatc
     1081 gcgtcgcccc ggacaacaca agtctcgaag gaaacgaagg acccgcaggt gggaaacacc
     1141 actcttatct cacttccatc cgccgtgcag tcagcgccgt cctgcttttc tgggttttct
     1201 acgccacgac tgccgaactc atccacatgc tctggcgcga ggcacccatc cccttctttc
     1261 ccgtagctgc gctggagccg ttccgcatcg caaatcaata tgggcttttt gccgtgatga
     1321 ccagaggccg ctacgaaata gagtttcaag gctcgaacga cgggcagaac tggactgcct
     1381 atcccttccg ctacaaaccg cagaagctcg atgatcctcc gcgcatctat gcgccttacc
     1441 agccgcgctt tgactggaac ctctggttcg cttcactcgg tagctggcag cagaactcaa
     1501 tggtcccgct gaccgaagaa cgtctgctcc agaacagccg ggacacgctc tcgctctttg
     1561 ccggaaatcc attttcgcag tcgcccccga agtatgtccg cgccgtattg tggcaatatt
     1621 ggtttacttc gatggcggaa agacgcgcga ccgggctttg gtggaagcgg cagcttctgg
     1681 gtctttacgc gcctgtgctt acaatcaatc cggacggaaa atttgaggcc gtccagtggc
     1741 cggagccact tcctcctcat gaatgacgac accagcctcc tcatcggatc gaagcacaag
     1801 catccgcttc atgctgtcgt catcgggtct ggccccaacg gcctttccgc ggccatcgtg
     1861 cttgctcagg caggctggca ggtagatgtc tatgaggcac agcccaccat tggcggcgct
     1921 tcgcgcaccc tgccattgac tcttcccggc tttcagcacg atttcggttc ggctatccac
     1981 gctatggccg ctggttcgcc gttcttctct actctgccgc ttgccaagca tgggctcgaa
     2041 tgggtccacg cgcctgcacc gcttgcgcat cctttcgacg acggaacagc catcacactt
     2101 gaacgcgatc tggacgatgc cgaacgtgta ttcgggagcg atggcaagcg ctggcgaagc
     2161 atcttcggcc cgcttgcaga gcactggaac cagattgcaa aaggtgcgct ccgtccgctg
     2221 gtccactttc cggcgcatcc gtttctcatg gcgcggttcg gcacgttttc tctgatgtcc
     2281 gcgcagttct tcgctgaccg tttcttacaa tcatcgcgcg tccgcgctct ctttgccgga
     2341 attgccgcgc actccatcat gccgctggat gcgccgctca gcgcctctgg cggagtcatg
     2401 ctggccgccg ccgcccatgc cgtcggctgg ccgatgccgc gcgggggggc gcaatccact
     2461 gcgaatgctt tggcaggata cttctcctcg ctcggcggaa gcatttacac ccattcgccg
     2521 atcacctcgc ttgaagactt ttcgccacat acggtcctgg tttgcgacat cagcccgcgc
     2581 cagttgctgg accttgccag ccaccggctc acgggtcaat tccagaagga tctgcgacgt
     2641 ttccggtatg gccctggggc cttcaagttg gactacgccc tttccgctcc cattccgtgg
     2701 aaggccgctg agtgcgcccg cgccggaacg gtccatctgg gcggaacaat ggaagaaatc
     2761 gccgaagccg aacttgcagt ctggcagaac aggcatccgg accggccatt tgtcctgctc
     2821 acgcagccta cgctgttcga tccctcgcgc gcacctgagg gaaagcacat cgcgtgggcc
     2881 tactgtcacg ttcccaacgg gtccacgttt gatatgtcag agcgtatcga agcacagatc
     2941 gagcgcttcg cgcccggttt ccgcgactgc atcctggcgc gccatgtctc ttcgccctct
     3001 tttcttgaaa cgctggacgc caacctcatt ggcggggaca tcaatggagg ggccatttcc
     3061 ctgcgccagt tggtcttccg gcctacaccg cgccattatg ctacgtccga cccgaacatt
     3121 tttctttgct cggcctcgac ccctccactc ggcggagtac atggcatgtg tggatatcac
     3181 gctgcacagg cggtgatccg ccatgtaaga cacagactgt aaagccctgg tgattctgac
     3241 gaccgaagtg gaccaccatc acgtgcagtt ttcgcttgat ggtggcaagc ggaggaagaa
     3301 tcctgatagc gcatcctgcg ccacgcagca cctgctttca ggggccgttc accgttattt
     3361 cattgattaa cgttctttat gctggccccg gcggcctcat tcgagttata agctgtataa
     3421 ataggacccg ttctcactac aaaaaaaagg acgttccagc gacatggatt ttgagcagtt
     3481 aaaaactttc ctggaagtca gccggctgaa gagtttctcc cgcgcggcag acaaactggg
     3541 cgtgactcag ccggccatct cggcgcagat tcgctcgctg gagaacgaag tcggggcgcg
     3601 cctctttgac cgcgatggcg gaaaagtgac cttcacggcc gccggacgtc tctttgagcc
     3661 gtttgccgaa cactgccttc aggcctcaaa ccacatcatg gtggccatcg gcgagctgca
     3721 ccgctcgcct cgcggagaga tttccatcag tgccaacgag gccaccagcc tttatgttct
     3781 tcccgccgtt tttgcgcagt tcaagaagca gtacacacgg gtgaacctct ccattgtgcg
     3841 cgcggaccgc tctaaatcac tggaagccgt cctgaatcgc gaagtagatt tcggtgtgat
     3901 ctcgcttccg gtgaaagacc cccgtcttct ggtcgaagtt gtccataaag atgagctggc
     3961 gctggtagta cccaaaactc atccgctggc cacccgcgaa cagatcaagt ttgccgagat
     4021 gcttcagtat ccgttgctgc tgcccaagca tggacggcag cgcgatcaga tcgaagacct
     4081 cttccgtacg catgatgtga atccgcgcgt cggcatggaa gtggattcga gcgaattgct
     4141 gaaacgcctg attggggcgg ggctgggaat gggctttctg ccacgttcaa acgtgcgcga
     4201 tgacgaacgc gccgggctgc tgaagacctt gaaagtagaa ggtgttcgac tcaaccggga
     4261 gcttgcgctc gtcttcagaa aagacaagac gctgactcgt gccgcccagg cctttctgga
     4321 aattgcgacg ggaagatcgc gacccatgct ggctgcggtg gggcacaagc cacggtgaaa
     4381 ttccctcaat tcacttgtgt gttacgcgcc attctgaacg caggggaaga atacaccact
     4441 gctgtctatt gttttcttcc ttgatatttc ggttgggcgg gcgaatgacg ctgctttgat
     4501 gtttctattc ttccagccgg taggtaaatt cttcaatcac agggttggta agcacatctt
     4561 tggcgatgcg ctctacttcc gtctttgcgg cggccgcatc gagcgagccg tcaaggtcaa
     4621 ggacaaaata ttttccctgg cggacgtcct ttatcccctt atattgcatc tttttgagtg
     4681 cgttctggat ggtctgtccc tgggggtcga gtaccgtccg cttgagcgtc acatagacat
     4741 gggccttcat tgattccgat tataaaggac tgcgatttgc gccgattctc acaaaacctt
     4801 aaagccgaat tgcgtacact ggagaagaat ggttaattat ctggaacttc cgattgggga
     4861 caaagtcccc gaggtcatca acagcgtcat cgagatccct ttcgagggca ttaacaagta
     4921 tgagtacgac aagcagttgc acgtctttcg tcttgaccgg aacctgtatt cgcccgtgca
     4981 ttatcccggc gactacggct ttattccttc gacactgagc gatgacggcg acccactgga
     5041 tgtgctcgta ctggtggacg cgcccagctt tcccggatgc gtcatgcagg tgcgtccgat
     5101 tggcctgctc gaaatgatgg accagggcgt tctggacgag aaagtcctcg ctgtgggtaa
     5161 gaacaatccg cgctacaagg acgtgtggaa ctattcggag atttatccgc acatgctcaa
     5221 agagattacg cacttcttct ccatctataa agacctggaa ggcaagcgcg tcgagattcg
     5281 tggttggcac gatgcttcct acgcacgcga ccgcgtcctg cgtgccgctg agaactttgc
     5341 aaacaacaaa aagcaggtcg aagtggcaaa gagataaggg ctgctgaggg actgcccttt
     5401 tcaatccttt ctcttgggct tgagtgggga gagaatgaac cccagttttt aaggcgcatg
     5461 cgctgcgtgc gcctgttccg ttttttcgct aacagataaa ttcagcttcc ggtcgaaagt
     5521 aatctcgccc gagacatcgc tcctggatgc gcccctgctt acttgcgatt tttccagaac
     5581 aagctgcgca ttcctgatga ctccgctggc gctccagtga tcaaaatgct ccagcgcagc
     5641 aggagcagca gccagagagc cttgcgtcca gtcccattgg aaggtcccct gagcagaatg
     5701 ctgtaaatca tcggcggaat agcctgccgc ctcaatgtgc gcgcgcaacc cgattgcgcc
     5761 ggatgccctt tcgccgtgcc acatactcgc gatctccgca gcgttggcgt gcgtcagttg
     5821 tgtatcgaaa ctgtattttg gcgtgccgga ggcgtccatc ctgcccgtcg catggagaac
     5881 gccgttccac gcatgaccat ccagtgtaag aaactgcact gccctgcctt caatgtgaag
     5941 ccgtccgctg gcgtcatgca cggccagagg tccaagcata aacgtgctta tgtggattgt
     6001 tccgttaagc gcaggccatt gcggtttgtt tctttccaga cgcgagagta tctgctgcaa
     6061 cagttcgttg tgttctcctg cgcctaacag agcagactgt aacgaggccg catccagatt
     6121 ttgcgcggta agagaaaact ccggcgcgca gttttctccg gagcagcggg ttgggatagt
     6181 cacggaaaga ctcacaggaa tcttctgaaa gaccgctgcc agaggagaaa ccacgacctg
     6241 ggtgggagaa agagagacct gcgcggaaac aatctcaact ggaccggaaa gaaacccggc
     6301 ctggtactct gcgtttttca gatgtagaac gccctcggtc gtcgtggccg gaacgggata
     6361 gtcagaagtg ctgacagggg atatccaggg accgtggacc ttcaggtcca gctccgcttc
     6421 tccttttgga gcaagcgccg aaacaatacc ggatgccaga tgcagtcccg atgcaatagg
     6481 acgcaggcgt tccattgatg cgtcaccctg gaagttcagg ctgaaacccc ttcgcgtaaa
     6541 gttgccggaa agttccaggg gcgaggcccc gccaagggga atgtcggcgt tctcaagctg
     6601 taaagccgcc ggagaggctg gctttggctg agatttttta cctttgcgag gtgcaggggc
     6661 agtctggcgg gcaacgatgt gcagggtagg aactgcaagc gggctgtcca tgtccggaag
     6721 ccggagggac aggtgctcga ctgctacatc gcctgtaagc tggtgcgcgt aatccaggtc
     6781 gccctgcgct gtgcctgaaa tctgcacgga atcagcgaat ttattgcgga agagccggag
     6841 cgccgaaagt ccaaatgccg ccggaacatt ctgcacgtga aggtgcaatg caggcacggg
     6901 atgctcgatg tccggaacat tgccggtcaa caacaagtgg ccgtcactga taggccagaa
     6961 acaggtaaga gcacgcaggg agcgctctgt ctgccggtat ccgccctggc aggtcgcatc
     7021 cacattgaat ggctcgactg gtgagaactc ctggcggtgc atgtctgtga tgcggatccg
     7081 cgtcttgaac tggggcccat acacactgcc ggtgatgtcc cctgtaattt caagattgcc
     7141 gcgccagccc gattccgctc ctaaaagtag gcggctgatc tgccccagcg ccgcattggt
     7201 ccactcagca tggagactga ctggcatgtc gccaagctcg gatgctcgct tcatcgagcc
     7261 ttctacgcgc agagtaccgg tgtctgccag atcgagatca atatcagtgc gcgcgggttg
     7321 ggcctcgagc cgaatgcgcc attcatcggg attttcaagc cacatggcga aatcggcatt
     7381 gaagaatgag aacggctttt tttctgcgcc aatcttgaaa ttcacccgtg tattgctggc
     7441 ctcaatataa ggaaaacgag gcgactggcc tgcgtgccgt tgggcggttg gcgcattggg
     7501 gatgtgcgcc gcctgcacga ggatggtccc cagattccag tgccccgaag catcgcgcac
     7561 gatgttcaca ctggcctgat cgaggctgat gcgtccgatc tcaagccgcc ccctccagag
     7621 cgaactgagg cggatggaag catctacgct gggcgcccgc agggcaggct ctgcgccgaa
     7681 ggcagggtct tcttcgacga cgaaatcgcc cagctccagc cccggacgcg gcagcagccg
     7741 caacgtcacc gatgagagat gcaccggccg tccgatggcg cggctgatgg cagcagcaat
     7801 ctcgccctga taacggttca ggttaatgag cggaggcaga acaaccgcaa gcgccagcag
     7861 taccactgcg gcaagaaata tccggagctt tgttttgggc ctacgcgggg tttcgctcat
     7921 tacactcact gctgattgca acatatcatc ccgcgcgggg gaaagctgct ttacccgctc
     7981 gccttatgat cggactgccc ggatacactg ctcacgacta tatggcatca gaacggtcta
     8041 ccctcccccg ttaaaggcca tccgtgatga catggtgccc gaagcgggat caggcctcaa
     8101 aacaggcccc gcttacgctt ctcgagttat agaattcgtc catgacaacg cttcaggatg
     8161 gaggcctgaa gttgctgcac agaattcacc cagtctcgaa aaattgcttc atgccatgca
     8221 gaagcagaaa cttcccgctt ctaagctcta ccgctgaaaa tgaccttatg cctgttgccg
     8281 ccatgaccat cgaaatccgg attgaacatg cgcaatcgct caaagaccgc aggcaggtag
     8341 tccggagtct caaggaaaag ctgaggaatg ggtttaacgt ctcggtcgca gaaacagata
     8401 aagcggtcac atggcagtcc gccacgattt ctgtagctgc ggtttccagt tcgcgggact
     8461 atctttccgg gctgatgcag caagtggaag atgccgttgt gcgcatcaca aacgaccttg
     8521 gcgctcaagt gacggatgcc tggtgggaat tcctggaaaa ctagaccgcc agatcaaaat
     8581 caaagaggcc tcctttgcgc aaaagagcgc gctgtcctta aagctgctcc agaaacttcg
     8641 caatctcagc cgtaacggag ccaatttcgc accctggggc catgtggccg cagtcagatt
     8701 gcagcaccag cgtctgcgca tggatgagct ttgcgaatcc aagagcaggc agcgggttta
     8761 ccatgtggtc ctgcatggag gagatgatga ggacctttgc ccgcaccttt tttgccgcgt
     8821 catagagcga accaccgtgt gcgatgtcct gcccgatcat ggcctgtagc tggcgcagat
     8881 aatcatttgc atcgaaaaaa cggtcaccct ctttggtgaa ctgctcgaag tactgcggga
     8941 aatcctcgcg tgtggtgtgg ttggcgcgaa agatgggcgt ggccaggttc atattgtgaa
     9001 tcagcgcgac gataggcaga ggctgtggct gcgtgtattt ccctccgtta tagccgggat
     9061 cagcttcgag tgcctttaat tctgtgttcc atagcagaag atcgtacgag gtctgctgcg
     9121 gtgtccctac caccggaatg accgcatcca tgaagtcgga atagctgacc atccattgaa
     9181 atgcctgcat cccacccatc gaaacgccaa gaactgcgcg cagatgctga agatgcagcg
     9241 tttcggtgac caggcggtac tcggcattca ccatgtcaat aatggaaaac tgcggaaaat
     9301 ccgtgccgtg ctgggtcgtg ctgttggagg gcgagcagga gacaccatct cccagcgcgt
     9361 ctacggcaat gacgaagtat tttgtgctgt caatgtagtg cccggagagc acctggatgg
     9421 cgatgtcgct gctgcgtccg gtaaaccagc tgggaaagag cactgcattc gactttgcgg
     9481 cattcagagt gcccagggtg cggtagccaa tcttgcagtc gtgaatgact gacccatttt
     9541 ccagcttcag gttccccagg tcagcgtatt gcagtggggg gatcggcgga ggcgtcggcg
     9601 cctgggccag ggcggccatt gaaaacagta agaaaacgga agaaaacaac acatttcgca
     9661 ttttggttgt tattctggtg cggcggaaag aatttccacc tcggggagtg aacgtttgcg
     9721 gcatacgatt tgttctgtcc ttgtatctgc ggtcatgata ctgtcttcca gccagttgca
     9781 tgcacaatcc ttcgtgaaca cgttgatgcc cgaaccggca aagcttacag cggaacaggg
     9841 cactctggcc attacaccgt cattcagcgt agcagcgcct catttccgcg acgcccgcct
     9901 cgatggagcc atcggccgca tgctgcaccg cctcgaatac gcaaccggag tccctgttgc
     9961 aaaggacatt cataaagacg caagcggcgc ttcctttgtg atagacgtcc gggggccggg
    10021 cgagacaatc cagtctgtag atgaagacga gtcgtattcg ctcgacgtca cccccagcgg
    10081 tgccactctg aaggccaaca ccgtggttgg tgcgctgcgc ggcatggaaa cgctacttca
    10141 actggtgcag gcgcagcagg ggaaatatgt gctcccagcc gtaagcattc aggactcgcc
    10201 gcgttttcag tggcgtgggc tgctgattga ctgcggacgc cacttcgagc ctgtagatgt
    10261 catcaagcgc actctggatg gcatggctgc ggtcaagctg aatgtcttcc actggcacct
    10321 gactgaagac cagggattcc ggattgagag caaggtctat cccaggctga ccgaaatggg
    10381 ttctgacggc ctctattaca cgcaggagca ggcaaaggaa atcatcgagt atgcgcgcga
    10441 gcgcggcatc cgcgtagtac ctgagtttga tatgccagga cacagcacaa gctggtttgt
    10501 tggctatccg gaaatctcca gcggccccgg cccatatcag attcgccgcg aattcggtat
    10561 ttccgactac gccatggatc ccacgcgcga cagcacgtac aagtttctgg acaagttcat
    10621 cggagagatg gctaagctct tccctgaccc ttacatgcac attggcggag atgaaaccaa
    10681 cggcgtgcag tggaagagca atccgaagat cgtcgccttc atgcagcagc acaacatgaa
    10741 ggacaccgcc gcattgcagg cgtacttcaa tcagcgcctg cttaagattc tgcaaaagca
    10801 ccacaagcac atggtcggct gggacgagat tctcaatcct gctctgccga aagatgttgt
    10861 cgtgcagtca tggcgtggtg tcgaatcgtt aagcgcaggc gcgaaacagg gatatcaggg
    10921 aattctttcc gctccttatt acctcgatgc catgaagcct gcaggcgaac attatctggc
    10981 tgatccgctg ccgtcttcgt ctgaccttac tccagaacag cggaagctgg ttctaggcgg
    11041 agaagtctgc atgtggggtg agcacgtgaa tgagcgcacc attgattcgc gcatctggcc
    11101 acgcacggcg gcgattgccg aacgcctctg gtctcctgag aatgtgcgcg atgtggaaga
    11161 tatgtatcgc cgtctggatg tgatgtccgt ccggctggag gcatttggcc tcacacatct
    11221 cacgcaggaa gatgtgggct tgcgcgctct tgccggcaca acggacatcc atgctctgcg
    11281 cttcttcgca tctgtgatcg agccggtaag ctttggagag cgctaccagg cccagcacac
    11341 ctcgcaactt actccgctgg accggctgat tgacgccgtg cgccctgatc cgccagcgaa
    11401 gcaccgcata gcttttatcg tgcaggcgtt ccttaaagac cccgccgggc atcaggatga
    11461 aaagcgcgag atcacgcttt tcttcagagg cctgcaagta ctcgctccga agatacagga
    11521 gcaggtcgcg acatctccgc tgcttgcaga agatgcgacc ttgcgggcgc agcagttgcc
    11581 cgaactcgcg cagattgctc aggatgccgt caggtatctc actggtgaaa ccgccccggc
    11641 aggatggaag caaagcagcc tcgccgcaat ggatgcaacg cagaagccat caggcatggt
    11701 gcggtttgtc tttttggatc cgctgcggca gcttgtgaac gccgtcccgg agaaataggc
    11761 tacggcattt ttacagattc tgcacactct atttcggact gtgcggtgtc gccgcgcggc
    11821 gactgtagtt tcatggcatg gtccagacca tctgtgaaaa tgcactttcc ggtcaggagt
    11881 ggcgacggtc ccaaaccatt gcgtcgaagg aatgcctctc ctgaaagcca atttttctgt
    11941 acagttccac tgcgtttgcg ttctcttccg tcacggtcag ggtcagtccc gtgaaattgc
    12001 gcgcgcgcag attctccgcg caggcctcaa tcaggcgctg ccccaggccc tttccgcgca
    12061 ggcttcttga gacgcatatc tgcgtgatgt gtcctacatc ttcgcgcacg cgcgaacaca
    12121 gcagaacacc ggcgagagcg ccgtctttgg tgcggacgac cgtgcgcgac gacagcggat
    12181 caaacaggcc gcatccagga aaacggacaa tgttatgcag aaagcgcaac gatccactga
    12241 cggtgcggta ctggtcatta atatagctgt cgagatgccc ttcgtacgcc tccgcgatca
    12301 gataacccga ggggtgataa tcgctctccc gccatggata tacagcgatg ccctccggca
    12361 acgcagagac ggcaggtggc gtgtaatggg acagatccag ctccataaag aggcgtcgaa
    12421 agagcttgaa gcccgcccgc tgaaatattt cctcgtgcaa accatgcgtg tgcagcagta
    12481 gctggcattc gatgcgttcg attcctggcg agtgctgtaa taactcaatc agatggcgca
    12541 acagctcctc ttccacgctg acagaaacat ggggaagcgt gaatacgtcg ccgataatgg
    12601 ctttccgttc ttcgtaaaca caaaaggcgt accctgcgat gcgcccattt tctaccgcga
    12661 catagccggg cagaatgcgc gaatcaagat attgcagcag cagatcggcc gaggagcggt
    12721 agtcccagtg cagacgctca ctccagacgc ggctttcctc ttcaagcaca ggccgcaggc
    12781 tgctggccga aaaatgccgg agatcgagaa tctcaagctg tagtgcagaa ctcacgctgt
    12841 ctatacctga aaagacagtc cctggtttca ataaggtctg tcattctgaa tctcagcgat
    12901 aatggcttgg tcaaaaacac tgagagtttc gccacgtctc attcctggcc ttgagtcact
    12961 gtcctggaag ctgctactgc gcagctacca tgtagaccac cagattctga gcaggataca
    13021 cgaagacagg ctcgtatcgc ctcccctcaa gtttttgacg cagatcgttc acataggacg
    13081 cacggacgac aagaatatgt tgttccggcg ggatgccatc tgtatcgtaa tttactgttt
    13141 tcttattgcg atagaacgca aggccgtatt ccatatcgcg ccggacctta taaacagcaa
    13201 cggtcccatc ggatgaaaca atctgttcca gcatcttcgc cagcgggcgc gcggaatagg
    13261 tgtaatcaat cagctgtact gtgccttttg tagacttgac ctcgccaaat cccaggaatg
    13321 gcccaatccc ataaaggaac agcacgagaa tgacaatcgg caccatcgtg gcaatgcgca
    13381 gccgcttcag cccaaagcga gcagttgtaa tcaggatgaa gatggtggcc gcaaaaccgg
    13441 tggttccggc agcaaacagg gcattgccag gcgggagctg gtcggtatgc tgaatgagcc
    13501 gcggcaacaa aagcaccagc atggtcagac agccaacaat gactccatgc agtaccagaa
    13561 gccagggctt aagacctttg gtccgcatcc ggttcaggta atcaccgcag agaatggtga
    13621 gcggcggaac ggaaggaaga atatagcccg gcaacttcga ctcggaaaac gtgaagaaaa
    13681 caatcggaat cagggcccaa agcacaagaa attcagggaa agcgtcgccc gcgcgcggat
    13741 gtcccatgta gtgcatcttc gcgcgtcgcg ctttccactc ggcaactgag tccttgatgg
    13801 cgtccgtcag cgcagcaatg gcgatgaccg tccacggcat cacgctgaga agtatgacgg
    13861 gaatgtaata ccagaaatgc tgctcgtgct caaagcggtt cgtggcaaag cgttcgatat
    13921 tgtgttccag aaaaaagacg cgcagaaagt tggggttgcg cttctgcacg gcgatgaacc
    13981 acggcagcac catcgcaaaa tacagcccga tacccggcca ccagatgctt cgcctgataa
    14041 tggaccactc ttttcgtaaa gcagcaaaga ccgtaatgat gacgatggcg agaaacgggg
    14101 ctaccggtcc tttggccagc gttgccgctc ccacaaagaa gtacagatca aagagccaga
    14161 atttgctgtc cgtctcatac catgcgtacc agccaagcat cccgatgcag aaaggcgcgg
    14221 ccagttgcat gtctgtcgaa gcgccgcggg caaagctcag aattcctgcg caggaggccg
    14281 tcagcaaggc ggcgtcaagc tgtcctcccg gacggaagcg ccgcatgtgc agataaatca
    14341 ggacaaccag gacaaaggca aagctggccg aaggcaagcg cgccgaccag tcatgcacgc
    14401 caaattcgcg gaaggcaaac atcgcccgcc agtagtagag cgcaggtttt tccagccacg
    14461 gcttgccgta cagaatgggc gtaatgtaat cgtgacgggc cagcatctcc cgcgcaatct
    14521 gcgcgtaacg tggctcgtcg gcgcccacca ggccaatacc gtcgcctccg aagatgggga
    14581 ccaggccgta aaaaagaaag aacgcggtaa acagaaaaag ggtcaatgtc tccagcgccg
    14641 tccccagaaa cgggaaacgg cccagaagtg tcgttggcgc agttcgcagc gtcttaatgc
    14701 ttttcggaat aggcacgttc gtttttatgg atttccggcg aattgctttc gagagctagg
    14761 ctatcagatt cgctcatgca acttcatgaa ctacacgtcc gagcatcttc tttgcaacgg
    14821 ccaggatcat tcccgcagcg tcaggcagcg gcccatcgag cgtgcatcca ctggcgtgcg
    14881 gatgacctcc gccgccaaag gcctcggcaa tatgggccac attcactttg cctttgctgc
    14941 gcagggaaag ccggacgcga tggttcggca actcgcgcag aaaaatggcg acatccaccc
    15001 cggcaatggc aatcgcgtaa tttaccagcc cttcgcagtc ttcttccgcc gcgttcgtgc
    15061 gaagcatgtc ctcatgcgtc acccacatcc aggcaatgcg gccttcgcgc cgcaggttcg
    15121 agagggctgc cccaagcagc agcatctttg aggttggccg cgagaaatac acgtccctgg
    15181 caatcgcggc cgagttggcc ccatgccgca ccaactgcgc cgccagctcg aaggtgtggg
    15241 cgtccgtgcc gtcatagcag aactggcccg tatcagtaag gaccgcagta taaagacaag
    15301 tggccatctc cggagtaatg tgtgctccgg caacgatggc cagctcgtag atcatttcgg
    15361 caacggcgca ggcttccgta tcaatccagt tgatattggc aaaggccttt ccgctcacgt
    15421 gatgatcaat attgatgagg aagcggcctt ccagaccatg caggcgcgag cgctcaatgc
    15481 catcgcattc cagcagaatc acagcatcgt agtcgctgtc cacgcgcgaa acctgccgga
    15541 tggccgaaga gcagggcaga aagcggtaaa taagaggcac acgatcacac gaaaccatct
    15601 caacacgctt cccgagctgc tccagaatcg tagcgcaggc cagcatcgaa ccgatggcat
    15661 cgccatctgg ccgcgcatgg gccgacacca gaaatttctg gccgcgttca atcgcgtcga
    15721 gtactgcgtg aatgttgtgc cgttccgcca tagtctttgt cactctgccc gaaggcaaag
    15781 gattctgtat aaatatctgg ataccccggg tccgtgaagc tagcctgggg tcctgctcct
    15841 cttcttcacc cttccaagca actcatcaat ccgggccttc atcttttctg accggtcaat
    15901 gtgaaacaca agatcaggca catgccgcac gcccatgcgt tccagcaatt catgccggat
    15961 atacccgcgc gccgccataa gcccttccag cgtgtcctct tcttctttgg ggccgccgtc
    16021 tacggcgaca taaatcagcg ccgactttcc accaggattc agcacaacct ggctgacata
    16081 ggcaaaactg atgcggggat cggacaactc tccctcgatc atggccccga tctcctcgcg
    16141 cagcgcctct gccacgcggt cctgatggta tttgcgcgcc cgttgttccg gcatctccaa
    16201 tttcctttga ttaaacgatt gagaatatca gacttggcgg tactcttgca tgatggcgaa
    16261 tggaaagcag aaaaagtcct gcccgaggac gtagctggaa cagagagcac cttgcttcag
    16321 gaaaaatgga tggtacgatt ccccggcccg cgttgctccg caagccgggt tgaatcatcg
    16381 ctgctcgttc agaaggtaaa cttcaatccc cattccgtct gtcgcgcggc acccagcgca
    16441 tacactcccg tgccattgcc cacgcggaag gtttcattgt ccagctttcc cgttccattg
    16501 atggctcccg gagaaccgga aaggaggccg ctcatggtat tgggtggtag attgaagttt
    16561 ccgaaattga gaagattgaa gaaggcgacg ctcggctgga tggtaaaacg ctctttgatg
    16621 gtcagcttcc agcctacgtt cagatcaagg gcgcgcagcc aggtaaagtt cgcctgatct
    16681 gaaggaggag tactgaggac tggcgcgacg ccatccggag ttgcagacga actgtttgtc
    16741 cctagcctct gaagctgggc cacggtcatc aagccattct gcacaaggac ctgtccggca
    16801 ggcgtgggct gacctccata ctttgcgttg taattgttga tgacctgggt cagcttttta
    16861 gcgtcaatcc cgcggtcaaa gttgcccatg tgtgtacctg gcatcaagtc ctgggtcgtc
    16921 ccatcacctg taaaatcgct gcgaaaaatt tcaccgggtc ccagcccgaa gttcggtata
    16981 accagcgcgc tggaaagcgg actataaaaa tgcgcaatca gacccaaatt gaacttccag
    17041 ggtaggtctc cgaatccacc cagggatatc tgatgggtgc gatcgagcag gttggggcca
    17101 aaataccggt tcgggtcatt gtaatcagcc gctccaatca cgaagtcctg gtcgctgtct
    17161 ccggctgttc ctgacgcctg tgcgcctcca gagttctcca ggcgcgacag gctgtaagaa
    17221 aactggaaat tcatacgctt catgttctgg accagcttga cctgcattgc gttatagatg
    17281 gaacggccac gaggttccag gaagatggcc gcagcctgac ctggatttct tcctccaaat
    17341 gcacacggca cgccgattgc ctgaatacag gcctgaccaa aatctgtgtc cgcagtcaaa
    17401 ccatggctgg caaaatcgga catgctggcc ccaaccgcgc cgttgctctg gtcataaagt
    17461 ccgggacatg ccgcgatggc ttggtcaata cttgttacac cacaggcagc cagcgtagct
    17521 gcaatagcag cctgagcatt tgctaagtta aagttaccga tatcaccgac gtggttttca
    17581 tcaataccca gcagagtatg ctcttccacg ttgcgcaggt aatccacact caataccatg
    17641 ccctgccgta attcgcgctg cactccaata ttgatttgta tagaacgagg cgttttatat
    17701 ttgggggcaa aaaagcccag cggaataccc gctccggccc caagccaact gctgccacct
    17761 ataaagtttg gattgggggc attcagatca agaggattgc cggcaagcac ctgttgccaa
    17821 aaggccagca cgccgggaat tgcctgtcct actgtgctcg tgcagacgct cggatcgata
    17881 gtgatcaagc cggcgttggt aggtaccggc aacgctgttc cggcattaca agccgggcta
    17941 acctgattaa atgctccggt ccttaaccgc aaaggccgat caaagagaac attgttataa
    18001 ataacgtttt catagtaaag gccaatgcct ccgcgtactg aggtcttgcc attcctgaac
    18061 gggtcccatg caaaacccat ttgcggtgag aaattagtat tcggcaaatt aactcggttc
    18121 ccaaacccgg gaaaggctgc gttgacttct ggaattgggc ccagatcgct gtccgtgcgc
    18181 cccgtgtccc ggtcataacg caatgccaga gtgattgtgc tgtttggaga aaacttccag
    18241 acatctccga aataaatacc gatacggtta tctggtccaa gaccgcctgc cggaaagccc
    18301 aatgccggct ccagtgtgct gaaacccaac ccattgccga ctctcacgtc atagaccgga
    18361 taattcaatg gattatttgc ccctccgtgg gcagcggcaa atgcttctgt cgcgctattt
    18421 acctgccatc tgattcgagg agcggtaccg taaaaattcg caaaccctcc tccctggatg
    18481 tggttccaca tcacgccata gcggagcaga tgtgccccca gtaatctgct gccgtcatat
    18541 ttgacctcgg tatttgcctg cggagtgctt tgaggggcca gcgggctagg gccgctgtaa
    18601 aagccgccag taacggaagc cagggttaca cccgtgttcg caaaaggcag actgctgcct
    18661 ttgacccggt ctacaatttg gttctgaaat ttcagatacg agaagcgaat gctgtggctg
    18721 tagtttccag ctacaaagtc gtagccaaca acgtgctgac gggtatagtc cttgttgtca
    18781 tagactgcat atcccaggcc aaaatcagaa ttcagagagt ttgcaaagta tgagaaacgg
    18841 tagaagagct tacccgagtt cccaacattg tagtccatcc ggcccaggac ttccgtttcg
    18901 cggaaaggaa cactgaaccc tccgttataa gaattaaagg gagaggcaaa ctggaccgga
    18961 gcaaaggagt cctgtttgat gcgctccgca tctccaaaga aaaagagttt gtctttgagg
    19021 accggaccgc cgaggtttcc gccgaactgc gaacgctgga agggaaggtt tgtcccgccc
    19081 ggggatgatg ctcccccgac cgaagcgtcg cggaaaagac cgaaggcctg tccgtgaagg
    19141 cggttggtac cggacttcgt agtcacgttg accgctccgg aggaggtcaa atccgtggac
    19201 atatccagcg aggactgtgc cagttgaaat tcctgaatgg cgcttgaggg aatgtcttcc
    19261 gtcgttgttc ctacagtctc atcgctcacg tccacgccgt ctacattgat gcgcgccgtt
    19321 cgcccaaagc gcccaccgaa ggaaatggac gagtacccgg ccttagttgg gtcgaagttc
    19381 tgtccgtcct gtatctgcac accgggctca agctgcgcca gatccaggaa gttccgtcca
    19441 ttcactggta gattgtcaat ctgctgggca ttcagcacgc cctgcacctg cgcctgctcg
    19501 gtattcacgg tgaccgcaga cccctgtacc tcaactgtcg tcgtcccctg cccaacagca
    19561 agtttggcat taacagtaga tgtatttccc acctggactg tgaccgcctg ggccatcgtg
    19621 ctgaatccgg gagcagaaat ttctaccttg tagttaccag gagcaatcgc gcccgagtta
    19681 taggccccgg atgaatttgt agttaaactc agtgcctgtc cagtaccagt gttggtgatg
    19741 acgacttttg catcgctgac gaccgctccc gaagggtcgg tcactgtgcc tgcaatgctg
    19801 cctgtgctga tggttgtctg ggccacaaga acaaccgcgc atgccagcag aaatagaggc
    19861 aggaggagat acttagcttg caaataccgg aggttcttca tgcctcatac atgaggcaat
    19921 ccttgtaccc gcaacgctca gaaccagcac atagttttga tctaaataaa aaataaagaa
    19981 atctttaaca tttagttcgc gccgtgtatt ttatttctaa taatatcctg taaataactg
    20041 ttgcttgtac agtaacttca cggatataga aaatgaaaga tttgtataaa aacttatatc
    20101 tctagagaga gcgcgcacgg cgattttcgt gtaaatacaa gcgtgactta ccggattcag
    20161 acaaaattcc cgccgtcttc ttccacatgc agcggcgttt ctttctttct gaggacatcg
    20221 aatcccgcgc gcagcccatt cagcaccccg gccacatgcc gcagaggcgt ggtaattcca
    20281 tgctggaccg cagccgtcgc ctgcgtgaca ccatccagga cggccgtggc catttcatct
    20341 acccgctgca cctgggcgtt cgcagccgtc gtgacttcat tgacggtgcg ctccacgcgg
    20401 tgcgcctgct cgcgcacaat atgtgaggtt tctaccagat tggctgtggc gactttgatt
    20461 ttcggggaaa catcttccag cagatttcgg gccgtcgcca gggcgggcac cagatgctgg
    20521 ttgatcttgt cagaaacctc tttggcctgc cgtaccgatt ttttgatagc aaagaacatg
    20581 cccagcagga caccagcctg caaaaggacg ccaaccgcga cagcgattgc aaaaattacg
    20641 aagaatacct gaggtaattg catctctctt tcctgcaaac gatcagaagt tggcgcctga
    20701 ttctcttaag aacaattact ggttcgcggc ttcctggccc gcttcagttg tggaacggta
    20761 tgcctggcgt ccggcctcga ccgcggcaga aacacgttca ccctgctcac tgacaaactg
    20821 gcgaccctga cttatcaggt cgttccactg tgcgcggccc ttctcaaaat actccttgct
    20881 gcggtccacg tattcaccta cctgcgcctt gccgcgctcg acgtaggcat tcacctggtc
    20941 agcggcttcc cgtgaacgct gccggatata ctcaccaccc tcgcgggcgc tgttcacaag
    21001 atcttcgcgg gtctctcggc ccgatttcgg ggcgtacaaa acgccaagca gcgcgcccaa
    21061 acccagtcct gccaaaaacc agctaaagcc attggatttt tcgtctgaca tatactctct
    21121 cctttacccg gaaaacggat ggtatcagaa cctttcagcg tataccaacg tcaatgacat
    21181 agtagatgcc gacacggccc tttccgctgc catctctcac ggggtcaatg cataacaact
    21241 gttttaccca tttcgcgttt gaaaggacta agatatttac ctgaaacctg tgccacgcac
    21301 gcagaaggag acactctctg gcatgaaaca cgcatacacc ataaaaacaa ggggcatcca
    21361 ggccgccctg ctcgccactg cccttgtact gggagccgga tgcaaccgcc agcaacagga
    21421 gcagccacag gcacggaccg accagcaggt cgcaagcgac attcaggcaa aaattgccgc
    21481 tgaaagcgca ttgaacggcc agcagattca ggtcagcgtc tcgaacggag tcgccacgct
    21541 ctctggcagc gcaaatgatg aggcttcgcg cgcgctggcc gcaaatgatg cgggcagtgt
    21601 caatggagtg aagaccgtca tcaacaatct gactgtcgtc cctctgccac agccgacaca
    21661 acaggcagca gctcccgcgc ctgctccgga acgcaaaaca tccgcagagc gcaaaaagaa
    21721 gcgctctgga caggaagtgg ctgaaaccgc tcccgctccc gctcctgcgc cagcaccagc
    21781 gccggctccg cagtcccagg cgcagcctgc tcctccgcca ccacctccgc agcctgtagt
    21841 gaaaacgatc acgattcccg caggaataat tgtccctgtg cgcattacgg aaggactcag
    21901 cagccagacc gcgcagccga accaggtctt ccacggctcg cttgccggag acctgattgc
    21961 tgacggcatg gtcgccattc cgcacggagc ttcagtggta ggccgcgtag tagatgccaa
    22021 agatgctacg catttttccg gcaattcact gctctccatt gagcttacgc agatcagcgc
    22081 ccacggacgt cagattcctg ttgtaaccga tgcctactcc cgccagggcc agggacgcgg
    22141 caagaacacc gccatgaaag ctggaggcgg cgccgccctc ggggccatca tcggcgcact
    22201 cgccggtggc ggcagaggcg ccgccatcgg agcagcagca gggggggcca ccggggccgg
    22261 agtcaacgcc attacccgcg gccagcaagt gcagatccag ccggaaacgc tcatcaactt
    22321 caagctgcaa tcgcccatca ctgtaacaac gtcgcaaaaa gctggagaag tccgctcctt
    22381 tgacgacaac agcaacccgc aattgcagaa ccgtccgcag cagtaaaaga tagcacttat
    22441 ggaaaacggc cccgcacaaa agcggggctt tttttcattg gcagctcgtc aaattattgt
    22501 atacatttac gaatacgtta aagtgtttta aattcccgga gggcgaaaaa tatgaaccgg
    22561 cgcagatttc ttcagggact ggccatcggc gctgccggca tgtctgcatg gggagatacg
    22621 cagaacgagc attctcctgt agttgcaaca accctggttt cccccattga tttgggtgac
    22681 cagaccttgc tttgctcttt tgaacacgaa ggccaaagct ggaaagttta cgaagacctc
    22741 cgcgttcgag atggtgcgtt cactttcgtc cgctctgatg ggcaaggaaa acaactacgc
    22801 aaaactgccg aggccacctt cgccgaaaac gggccgccgt atctgggaat accgctcaaa
    22861 gatatcggcc tttccgcgcg cgacctgctg gccgaccgcc tgctggagca tggcgaccct
    22921 gaccccgatc aggtcagacg ggccgcgcct ccgctggagt ctgcacctcc ggaacgcggc
    22981 ggctacaggc tgccgtggaa cactttcgtg ggtacgcggg aatgctctga taccatgcca
    23041 gtcttcccct caggaaacac ccggacctat caccccaatc aattcttccc cgatttgaag
    23101 cccggccttg ctcagagacg ttttgaagga cttattggcg gatggatgcc tgccgtccgc
    23161 aaagtcatgc cactctcagg ttctgcgtat tacgaactca tcatcttcgg agacgtgctt
    23221 gcgcgtgacc gcttcattgt ccagacatgg caccgcacgg cgctcatcga aaacggaaaa
    23281 atcacgaaag tagagtatgg atacagttat cctccgttcc ctccgcggcg acacgcaccg
    23341 cagcctgagg agttttaccg tggcctgctc ctcttcgccg cgtattggga agacctgctg
    23401 cgggacattg cctccgcgca tttgccggat gcaacttact ctgacatgac ccggcacgcc
    23461 tttgccaggg aactcatggt gcgtcccggc ggtgtctacc caaagtacgg ggcagtagac
    23521 cgcgattact atggctccga atacgatggc tttcaggaca tcttcaccag ctctgtcttt
    23581 gccaatcttg attggggccg ctttgaaatg gcccgtcagg tcatagacaa ctacctgacc
    23641 agattcactg acgacagcgg catgatcaat atgcgcggac cggaaactgc tcagtttggt
    23701 ctgaccttgt ttctgctcgc ccgctattac cagtacacag gcgatgcggc gcttctgctt
    23761 aaacaccgaa aaaagatcga agccactgcg tcccttctgc tggaactgca cgatgagagt
    23821 ctgcgtctgt ctcccagtga ccccggccat ggactcattc acggctggag cgagtccgac
    23881 tcctgcctgt ttccaaaacc cgatgtatgg tggcagccct atttttccaa cagcgccttt
    23941 gcggcgcgcg gtctttcgga aatcagcagc gtatggaaca gtctggacgc tcccggcatg
    24001 aaggccctgg cttccggatg gcggcagcgc agcgatacgc tacgccggcg cgtgatcgaa
    24061 agtgtcgaac aaagcgtccg gcgcgacatg gccccaccct acgtcagccc tctgcccgga
    24121 acaaagctga cctttcgcga atccatggaa caggagcacc ccagcccgca aggctggcca
    24181 catcgcgcct actccgagct tttgcaggcg gaagtgctgc cgcagcagct cgcaaacgca
    24241 gtcatagact gcatgagggc ctatggcgca accaccctcg gcgtggttgc caacgtaagc
    24301 cgtccgcgtg aaacgggacg ggacattctc ggctttatct catatggata cgcacgtgcc
    24361 ctgctcgctc tcgaccgcat cgaagaatac ctgctttttc tgtattcgca ccgctatcac
    24421 gaccacacgc caggaagctg ggtcgcgggt gaagtcgcag gcattaccgg agactcagcg
    24481 ctcttttgta tacccgcgca gctcacgatt ccattactgg tgcgctggat gctggtcttt
    24541 gaagatgccg atgaagaccg tctctatctt ggcagagcgc tgccacgttc ctgggtaggg
    24601 tcagaaaagg agatttccat cgagcacgcg cccacgcgat ggggacgcgt aagtttcagc
    24661 ctgaaaacga acatgactgc gaaaacagcg catgccagcg taacactcga aaaacaagga
    24721 gcgcatgaaa tttatcttcg tatgcgcctt ccggaaggag cacacctgct ctcagccaga
    24781 gtgaacggcc agcctgcaaa tctggccgga aagcaaaaag acaccattct cattcaggca
    24841 ggcaatgaga ggacttttga agtcgaagcg ctccttgcat gagtttgttg tcggtcctcg
    24901 ctcttcgcta cgtgaaggac caggacctgc ttctttcttc agcgcttctt ccacagtccc
    24961 gggtccacat cgtgaaatac attgtcccgg tcttcaattt ccttcagatg tttttctgtc
    25021 tccggtttca acccgcctgt tgctgcatac tcgtcccagg ctttttccac tgttttgaac
    25081 tggttcacgt gcgtctggaa gcgcatctca gcgtagtccc gcgcggcggc tgtcgtaatc
    25141 aaaaactgcc agtctgaaga ttccagcagc agaagttcac ggcaaagctg tttcatgatg
    25201 cgctcgccgg ttccaccatc gcgccagcgg ccttcggtcg caagttcgcg cacacgcgtt
    25261 tctgccggat agatgtgcgt ccaggtccac gccgtgtctg gattcagcca gacctcattc
    25321 gtcccgttct tgccccatga accttccgcc agtgccagat agcccgaggg cggctctttt
    25381 tccagataat ctccgcacgt cgtcagcgca ataggaatct cttctttcgc catgatgcgc
    25441 gccacatgct ccagccacag aggcccttca taccaccagt gcccgaaaag ctcagcatca
    25501 aatggtgacg taagaatggg cgtacggccg ttgccaaagc tgtccttcag cgcgtcatac
    25561 actacgctca caaagtggcg ggcgtggtct gctgtgcgtt ctgcggcacg ttcgggatag
    25621 tacggcgttt ttgcgcccag gtccacttta ggatgggtca cctgccaata ccggtgcccg
    25681 cccggccagc gctttttatg gaagtccaga taattgctgt cacccggata gcccatctcg
    25741 ccgctccaca cctggactcc ggtcttcgga tcgcgcggga agatcgccac agggtgctgg
    25801 tgcgcccgcg ggccatccac caaatagggc tgatagaagc catggccatt attttcgaca
    25861 atctcagtcg cggccgctac gcccaccggg cctccggcct tcagctcata cggcgtaaac
    25921 ttcaccgggc cttcgaccag gtgcgaatct acaaagaaat attcaatgcc gccttccgcc
    25981 aatgcctctt ccacaccaat gcgctcaaat gggggccaca acgccccgcc tgccggggcc
    26041 accggggcct gccagcgtcc tgccggacgg taaccgcact cgggtgccca gatgccgcgc
    26101 ggcttcttcc ccagataccg ctcatgcgtg gccgccgccg tcttcacctg cgcggtaata
    26161 ctttcatctg tgcccagcaa cgggaagtat ccatgcgttg cggcgcaggt aatcacctca
    26221 atctggccgg aatcattaaa gtgccggaac cccttgatga tatctccgcc cagttcttca
    26281 aagtccttca ggtcctgctc aaaaaaatcg agccagtagc gggcggtctg cgcaaagtgc
    26341 ttctcgccat tctgccggaa gtagctctcg tcctcgcacg cagccagaat cttccgcttc
    26401 agatattgag gaaattcttt tttgaaggtc gggtgcgcca actgctccag caggatcggc
    26461 gtgatattga tgttgcactt gagcgcaaga ccgtccgcct ccagcctgcg aaagacgcgc
    26521 agcagcggca aatatgtttc tgcagccgct tccagcagcc attccagacc atgcggccat
    26581 gttccgtgat ggacgacgta aggcagatgc gcgtgcagcg taaacgtcat atagccctga
    26641 ggacggcctg tcttcataga atccctttca gatacgaaat gtttgggttg aatcctggta
    26701 acgctatagg aagacgcgaa ctgctgtctg aacggttgtt tttgctggca atttctgccg
    26761 ctttgcaaaa agtctactat ggaaaggatg aatccggctt caaaacaagc cacgccagat
    26821 cacgtcgtca gcttcgcgga ggccgtccgc accgtccggc aggaagccga aaaaatacgc
    26881 gaagtacgtc ctgccgcaga acaagtccct ttattggatg caacaggaag ggtcctcgct
    26941 gaagcagtcc gcgccgaccg cgaccagcct ccatttccac gctccacgcg cgacggcttc
    27001 gcctgcatcg cggcagatgt ccaatccggc agaatgctca gaatcatcgg ccaggtacgc
    27061 gccggggaag catggcaagg cggcccactt cagccaggag aagccatcga aatcatgact
    27121 ggagcaccag tccctgatgg ggccaactgc gttgtcatgg tcgaacacgt tcaggctgaa
    27181 gagaatcagc ttcgcctgaa agccggccat agcattgcgc ctggtgaaaa catcgttcca
    27241 gcaggagcag aggcaaaatc cggaacagtc cttgtccccg caggaacacg cttggtccca
    27301 tctcacattg cagcagcggc ggcctgtgga tacgcgcaac tctccgtttt tcgaaaacca
    27361 cgcatcgcaa ttcttgctac cggagacgag ctggtttcgc tcgaaagcac accgcttctg
    27421 caccagatac gcaactcgaa cagctattcc ctcgcggcac agatccaact ggcaggcgca
    27481 gagccggtca tactacccgt cgcgcatgac agccagccag cgatcgaggc cgccatatgc
    27541 ggcgcccagg actgcgacct gcttctgctc tccggcggcg tctcaatggg caagtatgac
    27601 ttcgtcgagc aggtcttgca tcaccgcggc gcagagtttt tctttaccgg agcaaaaatc
    27661 caacccggac gtcctgttgt tttcggacgc cttccgcatc agtatttctt cggacttccc
    27721 ggcaatccgg tctcgacaat ggtcaccttt gcactctttg cccatccgct catcgctgcg
    27781 ctttcagggg aagcctctgg cgggtcacgt ttcgcgctcg ctggccttac gcatgaggcc
    27841 cactgcaagt ccggcttgac gcgctttctc cctgcccgcc tggattacag catccacgga
    27901 gcaacggcca ccgtcatccc gtggcaagga tcgggagacc tggccagtac cgccaacgcg
    27961 aatggatttg ccgtcctccc tgcggaagac aaaaggctcc cagcaggctc tttgatatcc
    28021 gtgctgctga tgtgacagcg tatttcatac atcagatatt gatcagaatc tatgaaacac
    28081 gctcaggaag aatccaccca aaggtggact taaaaacccg tccctgggac caagacgaaa
    28141 tgtgaacggt ttcctatcct gagaatgtct cttgataaat acataacctc ctgaccccgg
    28201 ttttccgcag aaaccggggc tttctttttg ttatgcgtgc cgcacaagct ggaccatata
    28261 aatcagggac aggaccgaaa cagtgatggc cgtcagccac gccgccacat tgaaccagcg
    28321 cgcgttaacg tgtgttccca tcaggtcttt gcgattgatg agcaacagca tgaacacaat
    28381 cacaatcggc agcaggacgc cgttcagcgt ctgcgagaag atggaaaact gcaccagcgg
    28441 gaaattcgga atcaggacca cagccgtacc gccggcaatc agcagtgtgt agagccagta
    28501 aaaaaacggc gcctgccgga atcctttatc tactccagac tcaaatccca gcccctcgca
    28561 caccgtatag gccgttgaaa ggggcagcac tgaggcggca aaaaacgacg cattgaacag
    28621 cccaaaggca aacaacagaa aggcgtaggg tccggccaga ggcttcatgg cctcggcggc
    28681 atcggcgggc atggcgatgc ttcccatgcc gtgcgcccac agcgtggctg cgcaggccac
    28741 aataatgaac cacgccacca cgtcagtaaa aatggagccg acaatcacat ccagccgcgt
    28801 ggctgcataa ttctttacgc tcacgccttt ctcaacaatg gaggactgca ggtaaaactg
    28861 catccacggc gcgatggtgg tcccgatcac gcccaccacc atgtagatgt aatcgtgctg
    28921 cgcccaggcc gcgcgtgaag gcagctttac ggtggcaacc atggcctcac gccagctcgg
    28981 cccggaaagc actcccgtaa tgatgtaggc gatgtaaaac actgacgcgg taagaaatat
    29041 cttttcgacg ctcttgtaat cgccgcgcac ggcaagcagc catacaagag ctgcacaaat
    29101 gggcacggaa atatatttcg aaagatgaaa aagctgaagg ctgcccgcaa tcccggcaaa
    29161 ctcgccgata atattgccga agtttacaat caccagcagc accatgatga tgaacgtcat
    29221 gcgcaggcca aattcttcgc ggatgaggtc gctcagcccc ttgcccgtca ccacacccat
    29281 gcgggcgcac atctcctgca cgataatgag cgccagagta atgggaatga tcgtccacag
    29341 cagcgaatat ccaaactgcg cccccgcctg cgaatacgtc aggatgccgt tggggtcatt
    29401 gtccacattg gctgtaatga agcccggccc aagcacggca aggaacagca gtatccgggt
    29461 tctccagcgt cgcagcatag tcagggacca aattaaaatt cagaagatgt ttgaatcgaa
    29521 acggccagtg ttcgcgccgc ttcttcaagg cagaattctt ttctcagttt tacatcaaat
    29581 caggatttca agaagggcca acccggtgcc tttcaggggc cttgatgaat gaccggccgg
    29641 ctctctctgc tactctgtct tcgatgccgc aatttaaatc aggaagcacc ggcgcgattg
    29701 cgctttccct gatcatcgcc gtcgcgtccg catggatggg catcggcgca aacgggattt
    29761 actgcgaccg cctgccttcc atgaccatca tggccacgct ggcggctttc gtcatcgcgt
    29821 tatgttttcg cctgtttgcg cgtgctgaca cccgcgctaa aaaggcccag cttgcgatgg
    29881 ccctcatcat cgctgccggg accctcttcg cggacacccg cttcgtgctg aaataccgcg
    29941 gcatctgcgg acagctacag cagcagatcc gccagatgag aaacccatca caataaaaga
    30001 catttccggt acccgctttt ttggcctttg gacaaaggca tcggtctacg cctttcgcac
    30061 cgtcctccgc tcaatcctct tttcgtactt ctcacctttg aggatgcgct ttacatagat
    30121 tcccggcgtc acaatgtggt cagggtcaag ctctcctggt ttgaccagct cttccacttc
    30181 agcgatggtg aatttcgccg ccgtcgccat catgggattg aaattgcgcg ccgtttttcg
    30241 gtaaataaga tttccggcag tgtcgccctt ccatgctttg atgagcgcaa agtccgcccg
    30301 cagccagcgt tccagcaggt aaggtctgcc gtcgaattcg cgcgtctctt ttccttccgc
    30361 aaccagagta cccactccgg caggcgtata aaatgccggg attccggcac cgcccgcgcg
    30421 aattctttcc gccagcgtcc cctgcggaac aagtttcagg tccaattctc ctttgaggac
    30481 tttttcttcg agcagtttgt tctcgcccac atagctgccc gtgtgcgatg caatcattcc
    30541 ggcctccagc atcaggccca tgccgaagcc gtctacgccc atgttgttac tgatggtgtg
    30601 cagccctttc acgccgcggc gtacgagagc actaatcagg ttttccggaa ttccgcacag
    30661 cccaaatccc ccgatcatga gggttgagcc atcaggaatg tcgcgtaccg cttcatcagc
    30721 gctggacact accttgttca cttttgctct cctgttgcgc gtaaaagcac tgagcagaat
    30781 acttcactca tgaggtgact gcatagccac acactgaatc caaatcttat ttaaggatga
    30841 ttcggaaatc gttcatagtt cgcaaaatgc gtatcttgcg gtacgatgtg tgcggattga
    30901 catcacagga ggacctgatg ctgaaaggat tccgggactt tattcttcgc ggaaatgtag
    30961 tagacctggc agttgcggtg gttataggcg cggcatttac acaggtagtg aattcgcttg
    31021 taaaaaacat tatcaaccct gtcatcgcgg cagtggcagg aaagccggac ttctcagctt
    31081 ttgtgctgga tgtacatggt ggaaagatca cctatggcag cttcttcaat gacgtcatct
    31141 ccttcctgct catctctgcc gccgtctact ttctctttgt gctcccggta aacaagctca
    31201 tgtcaaagtt cttccccgcc aaggcagaac ctcctaaaac aaagtcctgc ccggaatgcc
    31261 tcagcgatat tcctgttgcc gcaaaacgct gcgcgcactg cgcacaacct gtcgtataga
    31321 agaatggcgc ccaggaatac ctgagcacgc ggcgaaacag agcaggataa attttcgtcg
    31381 cgcatgcgat gaaaccagag cagaccagct tccggccatc tctggcttta ccggtggccg
    31441 tcttacatcg ggccccggaa tgcgttaact taaatcatga agcacgcgga aaaaggcaaa
    31501 gtgtatcttg tgggcgcagg cccgggcgat ccggagctgc tgactctgcg tgctgcttct
    31561 ctcatgcaaa cagccgattg catctttcac gatgacttgg ttgcccctga aattctgacg
    31621 ctcgcaggcg ccagcgcgct ggtggaaaac gtcggcaaac gctgcggcaa taaaaccatt
    31681 acccaggaac agatcaatca tctgctggta gaaaaagcga aggccgggaa cagcgttgtg
    31741 cgcctgaaga caggcgaccc tctgctcttt ggacggggcg gcgaagaaat tgaggcgctt
    31801 cacaaagact cggtgccgtt tgaaattgtc ccgggaatta ccgccggatt tgcggcagcc
    31861 gcggctttgg aggcttccct caccgaccgc cggctcgcat cgaaggtcat ttttcttaca
    31921 gcacaccgcg cgcaaagcgg cgaggaagcg agcttcggcc cgctcccgga agacgctaca
    31981 ctcgtcatct atatgcccgg ttcagattac gcccgcctcg cacgtcagct tggcgatgct
    32041 cgcgttgctc cggagacgcc ctgcatgatt gtctctggcg ccagcacgcc acaggagcaa
    32101 atactccata catcggtcgg cgacctggcg cagacggcaa aattgcccgc cccctgcgtt
    32161 gtgctcgcag gaaaagcact cagtatccat cgcataaaaa agacgggctg agatatcatc
    32221 agcccgtctt catctggaag caggtcctgc ttactgctgg ggggaggtga cctgtccttc
    32281 ctgcggcaat ttcactgtgc ctgtagattg ggcggtctgg ttatcactgc cgctcatatt
    32341 ggaaagcaac tgaatttcga cgcgacggtt cttcgcgcgt ccggagggag tactgttcga
    32401 ggccaccgcc ttgtctttcc caattccaac cagatagaag cggtgcggcg gaacgttgta
    32461 ctttgacgcc agatactgca ccacagcctc tgccctgcgc tggctgagag catagttgta
    32521 gtcggctgga cccgttgagt ccgtgccgcc ggtcagagca agaatgtagc tcttctgtcc
    32581 gttcagttga gcagcaaact catccagctt ctgtttgtca gagcgggtca gatcggcctt
    32641 gtcaaagccg aagttcacct gcacgtctgc cacctgcttg tagttgtcaa ggttggctac
    32701 aactcccgat agcgaatcca cgcggttcac cgcttcctgc gccgattgct gcgcctgcga
    32761 tgccgccgtt cctgcactct gcgcagactg ttgcgcctgg ttggcgctgt tctgcgcctg
    32821 tgtgatcccg gcctgcgcac gctggtctac atcgtgcaga ttgcggttgt ttgtggccac
    32881 cgcgtcatcg agttcgttgg tcttctgaat cactggtgtg gtctggctgc gaacatagtt
    32941 cttggtggtg cagcctgtgg ccaagagcag cgacaaggct ccgagcgccg aaacagcctg
    33001 aagtgtcatt tttcctgtgt gtgtcatttt cgttctcact ccattcccag agagcaagcg
    33061 cacgcctctc cctttacctc tgtccatcag gtctgcaagc cctgtgccaa atccataaac
    33121 gccatggaag tacttttttt tcaattgttt aatatctgtt tgcattcttt taaaaacatt
    33181 catccgggct tgaaaaccgg gtagctttta cccggctccg tgcaattcag ggaaggcaaa
    33241 agcgaagaaa cggtaaactg aaattcaggt ggatttacag gagaaaattc gcacattgcc
    33301 ggcgcgtccc ggcgtgtacc tgtacaaaaa tgcagatggc gaaatcatct atgtaggcaa
    33361 ggcaaaggac cttcgctcgc gtgtccgctc ctacctgctg gaagcctcac aggccaacgc
    33421 caagaccggc tctctgatgc gcgaggccgt agacctcgac tacattctgg tagataacga
    33481 gcatgaagca ctggcgcttg aaaacaatct catcaagcag cgtaagccgc gcttcaatat
    33541 tctgctgcgc gacgacaaga cctatcccta cgtgaaacta acgctcgccg accgttaccc
    33601 gaaggtcttt gtcacccgtc gtctgcgcaa agatggcagt gcctattacg gcccgtattt
    33661 tccaggaaac ctcgcccacc gcatcgtaga cctcatccac cgcagtttcc tcattccaag
    33721 ctgtaaagta gacctctcgc gctatcatcc acgcccgtgc ctgcagtact acatcaagcg
    33781 ctgcctcggc ccgtgcgtcg aaggactcac cacgcccgaa gcctatcgcg aagccgtgcg
    33841 tgacacccag ttgttcctcg aaggccgcgc agccgaactc ggcaagtcca tcgaagaccg
    33901 catgcacacc gctgccgcaa atgagcagta cgaactcgcc gcgaaatacc gcgacctgct
    33961 gcgcaccgtc tctgaattac aggaaaagca gcgcatcgca tcggcagaga atgacgatgc
    34021 cgacgtcttc ggctatcact ttgaaaatgg aatgctcgcc gtcaatctct tccacatgcg
    34081 cgtaggcaaa atcgtggacc gccgcgaatt cttctgggaa gatttgccgg agcttctcgc
    34141 ccaggatgga gacgaagagc aaatcgcaaa gcagtctgca aactttcaag caggaacatt
    34201 cttctctgcc ctgctgaagc agctttacat tgaccagcaa tatgttccgc gcagcatctt
    34261 cgtgcccgtg gacttcaccg accgcgaaac gctgtctgcg ctcttgagcc agcaaaccgg
    34321 gcacaagatt gaaattgccg tcccgcagcg cggagaaaag cgctcgctcg ttgatcttgc
    34381 cggacaaaac gcgaaacaag gctatgacca gcgcttccgc gtcatgcagc ccagccagaa
    34441 ggccatccag gaagcattac aggacgtgct tgttcttccg gaactgccgc gccgcatcga
    34501 gtgcttcgac atttcgcata ttcagggcgc ggagacggtc gcttccatgg tcgtctggga
    34561 agacggcgca atgaaaaagt ccgattaccg caaattccag ataaagactg tctccggcgt
    34621 agatgatttt gcggccatgc gcgaagtcgt cacacggcgc tataaacgcg tggctgaaga
    34681 aaacaaaccg atgcccagtc tcattctcat tgatggcgga ctgggccagc tccatgctgc
    34741 ggcctccgcg ctcgaagaac tgggcctcac cagccagccg ctcgcatcca tcgccaagcg
    34801 cgaagaaatc atttacctct acgggcagga ggacgatccc attgtgctcg accgccgctc
    34861 gcccgtattg catctggtgc agcgcattcg cgatgaatcc caccgcttcg ccatcgcata
    34921 ccaccgcaag cgccgcgaga tgcgcgaccg cgactccgag ctgctcacca tccccggagt
    34981 cggcgcccgc acccgtaccc gcctgctcga acacttcggc agcctgcgca gcgtgcagaa
    35041 tgctggcatt gaagccctta ccgcagtagt tccacgccga acagctgaac aaatctacgc
    35101 gcattttcac cctcacccgg catccgtcat tgtttctttg ccggctgacg gtcatagctg
    35161 ataacgcgtg tgatttttcc gcgtcccgtt cttgtatcac cggatccgat atatcgaaaa
    35221 tatcttgaat ccagactgta tttagatata tcttaagaca tatcaaagga gtatttgata
    35281 tgtttggaaa ctttacccat gaaggcggcc gctgcggaca ctggaaacat ttccgtggtg
    35341 gacggggctg gttcggtgaa gccggcgaat tctggggccg tcgcggacac cgcttcggcg
    35401 gcgacttcgg cggacggctc tttgaccacg gcgaactgcg ctatgtcatc ctgcagatga
    35461 ttgccgaaaa accaggctat ggctacgaga tcatgaaggt catcggggag cggcttgcgg
    35521 gcatttatgc tccaagtgca ggcgtggtgt atccaaccct gacgctgctc gaagaagagg
    35581 gcctcgccac cgtcacctcg actgaaggcg gaaaaaaact ctacgccatt actgaagccg
    35641 gcaagaaaga actaaaggcg aacaaggcac tggtggacgc catcttttac cgcatggaca
    35701 aggtcggtgc cgcatttgga cgcggccgct caccgcagat catgcgcgcc atgcacaact
    35761 tcagactggc actgaagctg cgctttgccc agggcgacct ctcaaaagaa cagatcaaca
    35821 aaatcgcgga gattattgat gcagccgcaa agcagattga gtccatctga ggaggacaca
    35881 ctacttgagc gtctgtatga gctggaagcc aaaaatctac agctacagac gctcgtagtc
    35941 gagttactgg acaagaacca acgcctgcgc aattccctgg cccggtatga gcagcaggtt
    36001 gaggtacaaa actaaaaatc ctgcctcatc gtattgagaa cctctaggct aaggacatgc
    36061 gcgaatttgt gtatcacctt tgcgatgtct ttaccggcac tcccttccag ggcaaccagc
    36121 tcgctgtatt tgagaatgcc ggaggtctga cgacggcaga aatgcagaag ctggccaacg
    36181 aaaccaatct ctctgagacc acattcatca tgcgccgctc tgcggaaatg gaagcaaacc
    36241 gcggcgtgcg cgtgcgcatc ttcaccacgc aggaagagct tcccttcgcc ggtcatccaa
    36301 cgctcggcac ggccagtttc atccgccgct ttcttcctga attttcagag cgcgaactca
    36361 tcactctcga actgaacgtg ggcgccgtgc aggtgcgttt tacttcgcct cggcacggcg
    36421 agatgacgca gcccaatccc gtctttggac agatgcacga acgggaagct gttgcccagg
    36481 ccatcggtgt ttccacagac gatatcgctt cagacatgcc tgtgcagact gtttcaaccg
    36541 gtatgccttt ctgcattgtg ccgtttcgtt cagtggacgc gctctcacac ctgcgaattc
    36601 ccggcgccct ggcagaagcc tatttaaaaa acaccgatgc gaaattcttc tactgcatca
    36661 ctcaggaatt gcaaggtgtg tggcgcgcgc ggatgcagtt ttacaacggc gaagatccag
    36721 ctaccgggtc ggctgcgggc tgcgcgattg cctatctggt gcgccacggc ttcgccgcat
    36781 ccggcgagca attgcacatt cgccagggca tcgaaatagg ccgcgcgggt gagatcggcg
    36841 ttcgtgcaaa attcgatggt gtctccgtaa gcgaagttcg cgtttccggc agcacaattt
    36901 ttgtggcaaa cggacgcttt ttccttgaat gattcacatg ctttcaacag gcctgaggac
    36961 gcgcatcttc aagcccagag cggagaaatc aaaatacggt gaatcttcag taaaaaattc
    37021 acacctcttt cgcttcatct tccctattta cacagcgctc cgggcttcgt actctcggaa
    37081 agcgttcaga aatcagaagt ggttctgaaa cgtaaacttt cgcctatatc acacaactga
    37141 ataagggccg gagcgcgtgg catatgcgcg ctttcaagcg gcttgtgcat acgtgcctgc
    37201 tatcttcccg cagcagtaaa agggaaccgc gcaggcgtgc agaaagctgc ttctgttgct
    37261 gcgctggcaa ccatgtccgc ctccggccaa ccgcaccccg aactgaggcg ggggatgcgg
    37321 caccgcgcac acgcgtggag gaaatttgct tccccaaaaa cctccacgcc gcgcggatgc
    37381 cctctctttg tcttttttcg cgtgtggatg atgtggaaca ttgcgggcca gatatgcgcg
    37441 cccgcacaac cgctttcgcc atgttcgcga agcagtcagg atgttatgag gccccgaaaa
    37501 gtaattcttt gcgttgatga caatgaacag gccctttccg ttcgcaaatt tatgctggaa
    37561 acacgcggct atcgcgtagt cactgctctc agtgctgaag aagcgctgga ggttttccgc
    37621 tcaggtggag tagatctggt cttgagcgat ctcatcatgc cgcagatgga cggcaatgag
    37681 atggtgcgcc gcatgaaaga catcgcgccg gacgtgccca tgattctgct cagcggtacg
    37741 gtcaaggcct ttgaccgcgc cagccatgcc gacgcctttc tgcccaaggg ggcctgtacg
    37801 ccgctcgaaa tgctggagcg gatccgcatc atgatcgcgc gcaaacgcgg ccccaggaag
    37861 gccaccccgc gcccattcta tcaacctcaa accgcgatgc cttccatcgc ctgaacagat
    37921 gcaggccccg cagttctgct gcggggtata gcattttcac cgatagcctg gaccatgccc
    37981 cgaaactaca gtcgccgcga ccaacagcga gtcaggcgac actgcacagt gcaaatggag
    38041 tgtattgaat ctgtgaaaat gccgtagtct ctcagaaact ctccgactgc gtggactcca
    38101 cgctcttgtc tcttatcttc gcgcaaccaa agatgcgaaa gtccgagaca tccttcaggc
    38161 ggaagatggg cgcgtggctg tcctttggga gccggatgcg gaagaagtcc gcttcgtgga
    38221 catcttcaag cacgaaggcc ggacgggcat cgggcgcggt ataggcaatc tcaacgtttg
    38281 tcagctcaac gttgttcacg tgccgcagat agaatccgtg cgcaggcagc ggcccgaaca
    38341 tatgcggatc aggataggcg tcttctttct ccggtacctg gatcgccgcc atttccgcgg
    38401 ttcccccgcc ctggtgatgc agatagatgt tgctgaattt gaggtcttct accgcatgac
    38461 ctgggattcc gctgacaagc acggcctggt gtgacgctga attgtaactg acaatgttac
    38521 tgaaaaggat gcgccgcgca tcgcccacag gaacaccctg cggccctctc atgcggctgc
    38581 ccagacgcac gaagatgggt gtgttcatga tgtcgcgcat ggtgatgttg gtaatggcaa
    38641 tgtcttcgag caatgcccca tctacgcttt ccagcgcaaa gccgccacag ccctcaaata
    38701 cgcagttgga aatcgtgatg tttttgaacc cgccattgga ttcggtgccg cacttgatgc
    38761 gtccggtgtg cgggatgcgc tgccccggcg gaaatttttt gaaggtccca tcgagcaatg
    38821 tgcccagctc atacgtgcca gtcacgtagc agttcgtaat ggttacattc tccgtggcgc
    38881 gcgcgtatcc cagagcatac gagcttttcg ggcagatgcc gtcgtcccac ggcgaattga
    38941 cgctgcaatt tgagacgcgg acattcttgc aacaatcaat gtccatgccg tcgcggtttg
    39001 tgtcaatctt cagattgtca atagtgaagt tgtctacgcc cgtcgcgaga atgccaaagt
    39061 gtcccccttt gaggatcgag aagtcgcgca gaataacatt gcggcagttc ttgagcgcaa
    39121 tggccttgtt gccgacacgc ggttcttccg cgcgcggctc ctcgtgcgac aatccgcggc
    39181 tgagtccttt gccataaatc aggcctggtc cgcagatggt gacatcgtgc agaccttcgc
    39241 cccagataag gctgttatgc cagtgattgt ggccgtaatc ctggtagttc tcccacgggg
    39301 cattggattc ggcaggatca tagccttcgt ttccggacga tggagtttcc gccgccagaa
    39361 tcgttgcacc ctgtgagatg tatagcgcga cattgctctt caggcgaatg gaaaaagaat
    39421 tgtacgttcc ggcaggaaag agaacagtgc cgcctccggc acttgcggca gcttcaatgg
    39481 ccttgttgat ggcgggcgag tcaatggtct tgccatcccc agtggcaccg aatgcgcgca
    39541 catcatagat gcttccatgc gctggcggag cggaggcggc aggctgagct ccgatcccag
    39601 gtggcgcaaa ggccccggct gctccaaccg ttgcatattt cagaaagtcg cggcgttgtt
    39661 tctgcatctg gtacgttctc ctggtggtct gacctcgtag aaacaataca gcaaggtgga
    39721 agaatttgtc tctgttgaat ttgtatacaa atatgtatac ttagcggcaa agacctgagg
    39781 ggatttttga tcgccatgag attgcgcatt ctttttgccg gcctatgttt cgctacatgc
    39841 ctttatggac aggtcaccgt tgccgactac gagcgcgctg accatctgcg cgacaaatac
    39901 aagggcctgg ccctgaacat ccccgaagaa ccgcactgga tcgaagacac cgacagcttc
    39961 tggtatcgca agacggtcga aggcggccac gcctttgttc tggtggatgc gaatgctctg
    40021 gcaaagcgtc ccgcgttcga tcatgaaaag ctcgcttcgg ccctctcttc cgccagtggg
    40081 gagaaataca cggcggtcac gctgccattc cagcgatttg aatttgttga caagcagacg
    40141 gccatccagt tcagaataga ccgccagcgc tggcgctgtg atcttgcaaa atatgtgtgc
    40201 gagaaggccg gcgcgcccga accggaagac gacgatggcg gttatgatga cactcccaaa
    40261 gctgtaaaca gcgagaccag cacaatcgca tcgccggatg gcaagttcga ggccttcatc
    40321 gagaactaca acgtctatgt acgcgcgaag ggcaagccgg aaaagaccgc gctgagctgg
    40381 gacggttccg aggacaatta ttacgcgttg agcacaatcg tctggtcgcc ggattcacgg
    40441 catcttgtgg cgtatcgcat ccggccagga tacaaacgcg aagtgcatta cattgaatct
    40501 tctccctcag accagcttca gccgaaatat tcttccatgg tctatcccaa ggccggagac
    40561 gtgcttgccc ttcctcagcc tgttctcttt gatgtgcagg agaagcgcgc catccacata
    40621 gacaacgcac tctttcccaa tccttatgat ttgtcccgcg cagaatggtg gaaggacagt
    40681 cgtggcttca ccttcgaata caaccagcgc ggacatcagg tctatcgcgt gattgaggtg
    40741 aatgcagcga caggcgcagc ccgggtgctg attgacgaga ccagcaagac ctttattgat
    40801 taccgcccgc tggttcccga tcagtacgac actggaaaaa gggtgcgcat agatgtgcat
    40861 gacggcaagg agattatctg ggcctcagag cgcgacggat gggaacacct ctatctgtat
    40921 gatggcgcta caggcaaagt aaagaaccag atcaccaaag gcgattgggt cgtgcgcgcc
    40981 attgatcatg tggatcccga aaagcgcgtc atctggtttg aagcgagtgg tatggagcct
    41041 ggcaaagatc cgtattacat gcatccgtat cgcatcaact ttgatggaac tggcctgacg
    41101 cctctctcaa accaggatgc agaccatcgc gtggtctttt cgcccgacgg gaagtattac
    41161 gcagttacgt ggtcacggat tgaccttgct ccggttatgg aacttcgccg tgccgatgac
    41221 aacaagacgc tcatgaccgt cgaacagggt gacattcaca aactgctcga tgcaggctgg
    41281 cgtccgcccg aagttttcac agcaaagggg cgcgacggca aaacagacat ctggggcgtc
    41341 atctacaagc ctgcaaattt agatccgcag aagaagtacc cggtcgtcga ggcgatttac
    41401 gccgggccgc aagggtcctt tgtgccaaag tcgttcggcc cgtgggcgca gccactgact
    41461 gagcttggct ttgtcgtcgt ccagatagat ggcatgggca caaacaaccg ctcgaaggca
    41521 tttcacgatg ttgcatggca aaatctgaag gacgcaggct ttccagaccg cattctgtgg
    41581 cacaaggcgg tggcggcgaa gtatccctgg tatgacattt cgcgcgtcgg catcttcggc
    41641 acatcggcag gaggacaaag tgcaatgggc gcgctgctct tccatcctga gttctacaaa
    41701 gtggccgtcg ccaacagcgg atgctacgac aaccgcatgg acaaaatctg gtggaatgag
    41761 caatggatgg gctggccggt cggcccccag tacgccgcat catctgacgt ggacaatgcc
    41821 tggcggctca aaggcaagct gctgctgatt gtcggcgaaa tggacaaaaa cgtggatccc
    41881 tcgtctacgt tccaggtggt caacgcgctc atcaaagcga agaagacctt cagcctgctc
    41941 gatgttcccg gaggcggcca tggcgctggc ggagaatacg ggcaacgtgt cctcgaagat
    42001 ttcttcgtgc ataacctgct gaaccaggag cctccggact ggaatgcaca gtgatgaaat
    42061 cacggaactg gtgtgccatg tacagtgtcg ccggctccct gttggtcgcg gtgactgtag
    42121 ttgcgtggca cggttagact ctccgtgaaa atgctataga aacagccaca taagccctgg
    42181 ctggctcagg cgtgtgtgga ccaccagaga agcgcgatga ataaaagctg cattgggatc
    42241 cgcaaccaga gcggcgttgg cggcttcccg cggagagtaa tgccctgttt agccgcattg
    42301 acgttggcga caaacatccc ggcaaggagc gcgataagac aaatggccgc aagtctgcga
    42361 aatgaaggga gcccaagccc gacggctccc agaagctcta gcacgcccgt gatgtagacg
    42421 atcagcaggg gatgaggaaa gtatgatggg atcatccgtg ccaggtcgtg cttcatcttg
    42481 ttgaaatgag ccgttgcggt gaagataaac atcacaatca aggcataacg ggcggactcc
    42541 tgccacgagg caaaagcgtg aacgccagcc gcccccatgg cgcgaaagac gagccacgat
    42601 atcaacagca cgatcagtac gagcatgagt ggtcgaatcc ggcttctgaa aggtacccca
    42661 cacgccagca atcttgcatt taggcgttat gcggagatct tagcaaacca acgcccagca
    42721 agagagctcc gctacagcat tttcacagat agtctggacc atgcccccaa actatattcg
    42781 cagcgggcaa cagggagcca gcgacattgc acggtgcaaa tggagtctac agaatctgtg
    42841 aaaatgcctt agcttcagtc ggagcaagcg aaagcacgga aaaaatatgg cggtattacc
    42901 gttgtagctt ttctgcaacc gacttggcct gcgtgaacag gtagaggtag tccggaccgc
    42961 cggccttgga atcggtgccg gacatgttga atccgccaaa cggatgcgct cccaccatgg
    43021 cgcctgtgca cttgcgattg aagtacaggt tgcctacatg gaagagccgt cgcgccttgt
    43081 tcagtttttc acggtcaggg gtgtagaccg cgcccgtcag gccgtattcc gtattgttgg
    43141 caatctcaag ggcatggtca taatccctcg ccttgatgat cgccatcacc gggccaaaaa
    43201 tctcttcctg cgaaatgcgc gcattcggag caatatcggc aaagaccgtc ggttcaataa
    43261 agtggccctt ctccggcgtc tcaatggcat tgccgcccgc aatcagccgc ccttctttct
    43321 tgccaatttc aatgtattcc aggatggact gcatcgcccc cgcattgatc accggcccca
    43381 tgttcggatt cagcgccgga tcgcccaccg tcagctttgc cacccgctcg cgcacgcggt
    43441 ctacaaaaac gtcgtaaatc ggctcctcca caatcgcccg cgagcaggca gagcacttct
    43501 gcccgctgaa tccaaacgct gaagccacca cgccctcgac ggcggaatcc aggtctgcat
    43561 ctgcgctgac gataatcgag tctttgcctc ccatttcaag aatggtgcgc ttgatccata
    43621 tctgcccggg cttcgtcttc gccgcgcgct cgtgaatatc cagcccaact tccttcgatc
    43681 cggtaaaggc gatgaagcgc gttttcgggt gctcgacaat ggcgttgcca aaggtggcgc
    43741 ccgagccggg gcagaagttc actactccgg caggcattcc ggcttcttcc agaacatcca
    43801 cgaaacgctc tgcgatggtt ggcgaatcgc tcgatggctt aaggacgacg gtgtttccgg
    43861 tgacaatggc cgccgacgtc atgcccgcca taatcgcaaa ggggaagttc cacggcggaa
    43921 tcaccgcgcc cacgcccagc ggaatataca gcagctcatt gcgctcgccc ggatactgaa
    43981 tcggcgtcgt cgccttcgag agccgcagcg cctcgcgcgc atagaattcg aggaaatcaa
    44041 tcgtctcgcc cacgtcggca tcggcctcgg cccagttttt gccgacctca tacgtgagcc
    44101 acgcgcagaa ctcgaactta cgcttgcgaa taatcgcggc agcattcaac agcagcgagg
    44161 cgcgctcttc ggcagaaaca aagcgccagg tctcaaatgc cttcagggcg gcctgcatgg
    44221 ccagttcggc gtgttccgcc cctgccttct gatggatacc aacaatctgg ctgggccgcg
    44281 ccgggttgcg cgattcaatc ttgcccgggg tcttgatccg ctccccgccg atgatcagct
    44341 catactcatg cccaagcctg tcaccaacat agaccagcgc ctctttcatc gcctgggcat
    44401 tttcatgctt cgagaagtcg gtaaacggct cgttgcgaaa ctcgccctgt ggcgagcgcg
    44461 gaaggatgac agtagagatt tcggctgtag ccataaccgt ctagtg
//
LOCUS       DASRBV010000338         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_174307, whole genome shotgun sequence.
ACCESSION   DASRBV010000338 DASRBV010000000
VERSION     DASRBV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174307"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFA02_12700"
     CDS             <1..454
                     /locus_tag="VFA02_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10755.1"
                     /translation="LGLLLAILCNANLLHITTKLWDSQAARDQVNAVAQMYSCNGGPE
                     CEALRKDYDRARRTIEEDLRALPLGYDLSYTKNYWRTFNQLDTAHNETRAHHTLKLLA
                     YWLIHLFGWLLTGIAVSLGAPFWFDTLNKFVNLRIAGAKPPKVDEARS"
     gene            569..1666
                     /locus_tag="VFA02_12705"
     CDS             569..1666
                     /locus_tag="VFA02_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZU10756.1"
                     /translation="MGPIVLKKDPCQWDSISLGEVMLRLDPGEGRIHTTRQFTAWEGG
                     GEYNVARGLRRCFGLKTAVVTAFADNPVGRLVEDFILQGGVDTSLIRWVKYDGVGRTV
                     RNGLNFTERGYGVRAAVGCSDRGNTAISQTKKGDFDWDSIFGLQNGSRWFHTGGIFAA
                     LSETTPQVAAEAMDAARKHGTIVSYDLNYRESLWKAIGGKAKAQEVNRELVRKVDLLL
                     GNEEDFSAMLGVQLEGVTEDFNELPIASYEKMLRDVAAAYPNLKMVATTLRTAHTASR
                     NAWGAMALYQDEIVHVPQSDVDILDRVGGGDSFASGLIYGILAGKPVEWAVQCGVAHG
                     ALAMTTPGDTSMATLAEVERVMKGGSARIAR"
     gene            1754..2401
                     /gene="eda"
                     /locus_tag="VFA02_12710"
     CDS             1754..2401
                     /gene="eda"
                     /locus_tag="VFA02_12710"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZU10757.1"
                     /translation="MSSEIKAEIERVGLVPVLRAKSAAEGHALVEAMLAGGVTVVEVT
                     MTVPGAVELLRELKKTYAGKLLLGSGTVTAAVQAAATIEAGAEFVVSPSLHPEVIAKT
                     KELGKVSIPGALTPTEVITAWNAGADYVKVFPCSAVGGASYLKALLAPFPQLQLIPTG
                     GVTQQTAADFLKAGARALGVGADLVNAKAIAEGKPEVVTNAARAYLEIIRQARSS"
     gene            2560..>2924
                     /locus_tag="VFA02_12715"
     CDS             2560..>2924
                     /locus_tag="VFA02_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU10758.1"
                     /translation="MGILDRFRLDGKTALVTGSASGLGAAIAMALAEAGASVACHGNR
                     RSAETTAKHIRELGRESQSFSADLSLPDGPEKLFALVSSAMGAPDILVNNAGMIYRNA
                     AEEYEYEAWNTVLQVNLTSV"
BASE COUNT          686 a          691 c          951 g          596 t
ORIGIN      
        1 actggggctg ctgctagcga ttctgtgcaa tgcgaacctg ctgcatatta caacaaagct
       61 ctgggattcg caggcggcgc gggaccaagt gaatgctgtt gctcagatgt atagctgcaa
      121 cggaggtccg gagtgcgagg cactgagaaa agattatgac cgggcacggc ggaccatcga
      181 agaagactta agggcgctcc cgctgggcta tgatctgagc tacacaaaaa attactggcg
      241 cactttcaat caactggaca cagcacacaa cgaaacgcgc gcgcaccaca cactcaagct
      301 gctggcgtac tggttgattc atctgttcgg atggctgctg acgggaattg cggtttcgct
      361 gggcgcccct ttctggttcg atacgctgaa caaatttgta aacctgcgca ttgccggcgc
      421 gaagcctcca aaagtagacg aggcgcgcag ctgaatcagt ccggaaaaga atgaagagag
      481 ttgacatctg tccacaaaga caattaccaa tccttccaaa tcaagaatgg tacggcgtac
      541 cggatagagg tacgcaggga aggagagaat gggaccgatt gttctgaaga aagacccctg
      601 ccagtgggac agcatcagtc ttggtgaagt gatgctgcgg ctcgatccgg gagagggaag
      661 gatccatacg acacggcaat tcactgcgtg ggaaggcggc ggcgaatata acgtggcccg
      721 cggactgcga cgttgttttg gcctgaagac cgctgtggtg acggcatttg ccgataatcc
      781 cgtgggccgg ctggtcgaag actttattct gcagggcggt gtggacacct cgctgattcg
      841 ctgggtgaag tatgacggcg tggggcgtac cgtccgcaac ggtctcaact ttacggagcg
      901 tggatacggg gtgcgcgcgg ctgttggctg ctcggaccgc ggcaatacgg cgatttcaca
      961 gacaaagaag ggcgatttcg actgggacag catttttggc ttgcagaatg gttcgcggtg
     1021 gttccatacc ggcggcatct ttgccgcgct ttctgagacg accccgcagg tggcagcaga
     1081 agcgatggat gcggcaagaa aacatgggac gattgtttcc tacgacctga actatcgcga
     1141 atcgctatgg aaggccattg gcggaaaagc caaagcacag gaggtaaacc gcgaacttgt
     1201 tcgcaaggta gacctgctgc ttggcaacga ggaagatttt tcggccatgc tcggtgtcca
     1261 gcttgagggc gtgacggagg acttcaacga acttcccatt gccagctatg aaaagatgct
     1321 gcgcgatgtg gcggcggcct atcccaacct gaagatggtt gcgaccaccc tgcgcacggc
     1381 gcacacggcc agccgcaatg cgtggggggc catggcgctg tatcaggatg aaattgtgca
     1441 cgtgccacag agcgatgtgg acattctgga cagggtgggc gggggcgatt cgtttgcatc
     1501 tgggttgatt tacggcattc ttgcaggcaa gcctgttgaa tgggccgtac agtgcggagt
     1561 agcgcatggc gcgctggcga tgacgacgcc tggagataca agtatggcca cgcttgcaga
     1621 agtggaacgg gtgatgaagg gtggttcggc gcggattgcg cgatagagtc cggcattctt
     1681 gtgtcatccc tcacatctct gcgagatgtg aggcgcccgc aattgggaaa gcggaatttg
     1741 attacggagg attatgtcat ctgagatcaa ggcagaaatc gaacgtgttg gattggtccc
     1801 tgtgttgcgc gcaaagtccg ctgctgaagg ccatgcgctg gtggaagcga tgctggcggg
     1861 tggagtcacg gtagtggaag tgacgatgac ggtcccggga gcggtagagc tgttgcgtga
     1921 attgaagaag acgtatgcgg gcaaattgct gcttggttcc ggcacggtga ctgcggctgt
     1981 gcaggcggct gcgacgattg aagccggagc ggagtttgta gtcagtccca gcctgcatcc
     2041 ggaagtgatt gcaaaaacga aggagctggg caaggtctcc attcccggag cactgacgcc
     2101 aactgaggtg attacggcct ggaatgccgg cgcagactac gtgaaggtct ttccatgctc
     2161 ggcagttgga ggcgcgagtt acctgaaggc gctgctggcg ccgtttccgc agttgcagtt
     2221 gattcctaca ggcggagtga cgcagcagac ggccgcagat ttcctgaaag ccggggcgcg
     2281 cgcgctggga gtaggagcag acctggtgaa tgcgaaggcc attgcagaag ggaagccgga
     2341 agtggtgacg aatgcagcga gggcgtatct ggagatcatc cggcaggcgc gaagttcgta
     2401 agtgttcaca agtgctacaa atgtctgcgc tgtaccggaa tgcggtacga ttgcgacaaa
     2461 aatggaattc agtcgttttt aaggaattga caatcgtttt tctaataaaa aagaatgctt
     2521 agcgtcaaaa agaacaacaa aaacaagtag ccaaaaagaa tgggaattct ggaccgtttt
     2581 cgtttagatg gcaagactgc cttagttaca ggctcagcca gcgggttagg cgccgccatc
     2641 gccatggctc tggcggaagc gggagcgtcg gtggcctgcc atggaaaccg gcgttctgcc
     2701 gaaacaacgg caaagcacat ccgggagctg ggcagggaat cgcagagttt ttccgcggac
     2761 ctgagtttgc cggatgggcc ggaaaagctc tttgccctgg tttcatctgc aatgggcgcg
     2821 ccggacattc tggtgaacaa cgccggaatg atctaccgca atgccgcgga agagtatgaa
     2881 tacgaagcgt ggaacactgt gcttcaggtg aacctgacca gcgt
//
LOCUS       DASRBV010000339         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_633819, whole genome shotgun sequence.
ACCESSION   DASRBV010000339 DASRBV010000000
VERSION     DASRBV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633819"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..945)
                     /locus_tag="VFA02_12720"
     CDS             complement(226..945)
                     /locus_tag="VFA02_12720"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZU10759.1"
                     /translation="MKRLPLVRLLAALGIVWVVTAVPCHAQGTSNDSSAPADGVNRSY
                     ASEPFWMEHGRLTFGFQMSFGLENNIPHDISHINMIFAEPQIGIIAWDSPYSRLPVRR
                     FELISEGILGASSHPGGRLFGTALLLRFGFKPIGRVVPYFDAGSGPVHTTIDEHAPEL
                     TGHTQFLSQGGAGLQYFLKPQCALVFEYHYFHMSNAGLQAPNPGFNGNMVTIGFRWLR
                     TPRLLAFRSLHRGRRRSTSTP"
     gene            complement(1067..1813)
                     /locus_tag="VFA02_12725"
     CDS             complement(1067..1813)
                     /locus_tag="VFA02_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU10760.1"
                     /translation="MRHSFALIIPALDEVESIGILVRRLPRELFGQIIVVDNGSRDAT
                     ASVAAAAGADVVREPRRGYGQACLAGIQHLRPQITAVAFMDGDLSDDPDDLARMVQCF
                     EQDEWDMVLGSRVLGRLEGGSLTPMQRFGNWLSTRLIALAWNVDFTDLGPLRIIRRDS
                     LARLNLRDRTFGWNVEMQARAAQTGLRICEVPVNYRRRAHGHSKISGTITGSLRAGFK
                     ILWTIFRCGLVPLPASSTGHRPQPRMDPFI"
BASE COUNT          473 a          560 c          629 g          420 t
ORIGIN      
        1 gagcagcggc gactgctgga atcattggca ccagccgtcg agattgaaaa caaacctgtt
       61 gagcgcgaac attccgaaaa agtcaaagac gtcttcgggt gatcgttcgc ttgtttcttg
      121 aattattgtt ctccttcatc cgggagggct cctactgtag catacgtttc tagctcaccg
      181 cctgccataa aaccgtgctt ccccttttgg ttgcgtcgcc cgtgcctatg gagtgctggt
      241 tgaacggcga cgcccacggt gcagtgacct gaacgccaac agacggggag tccgcagcca
      301 gcggaacccg atcgtgacca tattgccgtt gaatcccgga ttgggcgcct gcaagcccgc
      361 gttcgacatg tgaaagtagt gatattcaaa aaccagggcg cactgcggtt tcaagaaata
      421 ttgaaggccg gcgccaccct gtgaaagaaa ctgcgtgtgg cccgtgagtt cgggtgcgtg
      481 ctcatcgatg gtcgtatgca cagggcccga gcccgcatca aaatagggaa cgacccgccc
      541 gatcggcttg aacccaaaac gcaggagcag ggccgtgccg aacaatcgtc cccccggatg
      601 tgaggacgcg cccagtatgc cctcgctgat aagctcaaac cgcctgacgg gaagccgcga
      661 gtaaggtgag tcccaggcaa taatgccgat ctgcggttcg gcgaagatca tgttgatgtg
      721 ggagatgtcg tggggaatat tgttttcaag accgaagctc atctggaaac cgaacgtcag
      781 ccggccgtgt tccatccaga agggctcgct ggcgtacgat ctgttaacgc cgtccgcagg
      841 ggccgaagag tcattgctgg taccctgagc atggcaggga acagctgtga cgacccagac
      901 aattcccaat gcggcgagaa gccttacgag gggcagtcgt ttcattttga gggttctccg
      961 cagtctgttc aacgcaagag agcgcagaag gctttgttac cgtattgctc ttagaaaatc
     1021 aatgaaaatg tttgcttcca gtcgcggttc gcagcctccg acttacttag atgaatggat
     1081 ccatccgtgg ttgcggtcgg tggcctgtcg aagatgcggg cagcggaacc agtccgcaac
     1141 ggaaaatggt ccacaagatt ttgaatcccg cgcggaggct gcctgtgatc gtgcccgaga
     1201 ttttcgaatg gccgtgcgcg cgacggcggt aattcacggg gacttcacaa atcctcaggc
     1261 cggtctgcgc cgcacgcgcc tgcatttcca cgttccatcc gaaggtgcga tcgcgcagat
     1321 tgaggcgggc cagggaatcg cgccgaatga tgcggagcgg gccgaggtcc gtgaaatcga
     1381 cgttccacgc cagagcaatc aggcgcgtgg aaagccagtt tccgaaccgc tgcatgggcg
     1441 taagagagcc gccttcaaga cgcccgagga cgcgcgatcc cagaaccatg tcccattcgt
     1501 cctgttcaaa gcactgcacc atgcgcgcga ggtcatcagg atcgtccgaa aggtcgccat
     1561 ccatgaaagc tacggcagtg atctggggac gaaggtgctg aataccggca aggcaggctt
     1621 gcccataccc gcgtctgggt tcgcgtacta catcggcgcc tgcagcggca gctaccgagg
     1681 ccgtcgcgtc acggctgccg ttatcgacaa caatgatctg gccgaagagt tcacgcggca
     1741 gccgtctgac cagaatgccg atcgattcga cttcatccaa tgcggggatg ataagtgcaa
     1801 aagagtggcg catacgggtt gagaggccag acgcataatt ttaccattcc gcgagcggac
     1861 accatgctgt attctgcaga ggcaggactg gggcggtggg ttttctggag cggcggaaag
     1921 cgaagacggc gaggcggaag tccagctttt gcggatggat tcgctatatg atggtgtcgg
     1981 gctcgggatt tatggtataa aattacatta cagagcgatg cagcagggat attacccaga
     2041 cgaatgcttt cctcgtgcgt ctgaagcaaa cgccagcaag ta
//
LOCUS       DASRBV010000340         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_330181, whole genome shotgun sequence.
ACCESSION   DASRBV010000340 DASRBV010000000
VERSION     DASRBV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330181"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFA02_12730"
     CDS             complement(<1..350)
                     /locus_tag="VFA02_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZU10761.1"
                     /translation="MSTSAIQPVRAKRTGSASDPLWYKDAIIYELHVRAFYDSNNDGI
                     GDFPGLIQKLDYLQDLGVTCLWLLPFFPSPLRDDGYDIADYLTVHPNYGTLDDFKRFL
                     REAHDRGMQVMIELV"
     gene            complement(357..2351)
                     /locus_tag="VFA02_12735"
     CDS             complement(357..2351)
                     /locus_tag="VFA02_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZU10762.1"
                     /translation="MVKPKDGRKRVVIEHVSPELDAGRFPIKRIVGDTVHVEGDVFGD
                     GHDHVACRLLFRHEDERNWSSAKMEALGNDRWRASFTVTKIGRYVYTIAGRIDHFDTW
                     RSDLIKRLEAAQDVTLELQTGATMVEQAATRARGKDAAQLKEWAQILKTRSDSEEART
                     TALDPALAAAMERYPDTSLETKYERELSIVVDREKARFSSWYELFPRSCSPVPGKHGT
                     FADVEARLDYVSRLGFDVLYMPPIHPIGRSFRKGKNNTVHAEPGDVGSPWAIGSEKGG
                     HTAVLPELGTLEDFRQLVKKANEHGLEIALDIAFQCSPDHPWVKEHPEWFRKRADGSI
                     QYAENPPKKYQDIYPLNFESKDWSGLWDELKHVFLFWIEQGVRIFRVDNPHTKAFAFW
                     EWVIDEIKSEYPDVLFLSEAFTRPRVMERLAKLGFSQSYTYFTWRNTKEELTEYLQQL
                     THSELREYFRPNLWPNTPDILPEHLQTGGRPAFIARLVLAATLGTNYGIYGPAFELLE
                     NAPFRQGSEEYLNSEKYQLREWDLDAPHSIAPVLEKVNRARRENAALQTSHMLTFHET
                     DNPHLICYSKQSMDGANTVLIAVNLDPNYVQSGWVNLDMERLGLHAHEPFQVEDELTG
                     NRYLWEGQRNYIELSPHAVPAHVFRVLQRVRSEKDFDYYL"
     gene            complement(2441..2950)
                     /locus_tag="VFA02_12740"
     CDS             complement(2441..2950)
                     /locus_tag="VFA02_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU10763.1"
                     /translation="MIMTADDLKSLLHLKPHPCEGGWFAETWRAEEIIPKVSLPTRYS
                     SERAAGTAIYYLLEPHTFSEVHRLASDEIFHFYLGDPVEMLQLWPDGSTQTVILGHDL
                     AAGMQVQAMVRKGVWQGSRLRAGGKFALLGCTVSPGFDYADYEAGRRNELIAQFPGAA
                     DIIRALTHE"
     gene            complement(2958..3596)
                     /gene="thiE"
                     /locus_tag="VFA02_12745"
     CDS             complement(2958..3596)
                     /gene="thiE"
                     /locus_tag="VFA02_12745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZU10764.1"
                     /translation="MPLSIPSFYPILDTSFLPSGNREERLRQLVTELADAGVSLLQYR
                     NKQSEEAEILKDAAVMYQSAGGRVRLMMNDRPGLAVRAGFDGAHVGQQDMNPEEARKI
                     LGPDRILGVSTHNRAQIEIANTQPVDYIAIGPVFATSTKANPDPVIGLEGVREARKLT
                     AKPLVAIGGITLENARQIIDAGADSVAVISALFGPDERVGEVARKFLSAVKP"
     gene            complement(3711..3787)
                     /locus_tag="VFA02_12750"
     tRNA            complement(3711..3787)
                     /locus_tag="VFA02_12750"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3751..3753),aa:Asp,seq:gtc)
     gene            4138..4596
                     /locus_tag="VFA02_12755"
     CDS             4138..4596
                     /locus_tag="VFA02_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZU10765.1"
                     /translation="MAKSVNKVILLGNVGKDPEIRATPNGTIVASFSLATTDRTKDQS
                     GNWTDKTEWHNLVAFQRTAEIIRDYVKKGSKLYVEGKIQTRSWDDKNSGEKKYRTEIV
                     VNDLSLLSGRGEGETGGYSRSSNTASFNQSAPAPDDLSQAAEISDDDIPF"
     gene            4623..>5353
                     /gene="hemQ"
                     /locus_tag="VFA02_12760"
     CDS             4623..>5353
                     /gene="hemQ"
                     /locus_tag="VFA02_12760"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HZU10766.1"
                     /translation="MPELPSVPITLEGSSVLHQMFHFDWSSWKKLPSEEKQAVISEAV
                     GLFARWEQDSAGQSAAFSLLGHKGDLLLVHFRKSFADLGRAQLGLAQTKLHDFLKASH
                     SYLSVVELGLYESSAKTYAALAEQGLEPGTPEWDAGVGEVVERQSKAMAQRLYPAIPD
                     AKYICFYPMDRKRGEQVNWYTEPMADRQSMMHEHGMIGRRYAGQVRQIITGSIGFDDW
                     EWGVDLFANDPLVFKKLIYEMRFDRV"
BASE COUNT         1260 a         1485 c         1323 g         1285 t
ORIGIN      
        1 accagttcga tcatcacctg cattccacgg tcgtgcgctt cccgcagaaa acgcttgaag
       61 tcatccagcg tcccgtagtt cggatgcacg gtcagataat cggcaatatc gtaaccgtcg
      121 tcccgcagtg gcgacgggaa aaaaggcagc agccacaggc aggtcacgcc caggtcctgc
      181 agataatcga gcttctggat cagcccggga aaatccccga taccgtcatt gttgctgtca
      241 taaaaggcgc gcacatgaag ctcataaata atcgcgtcct tataccagag cggatcgctg
      301 gcgcttcctg tgcgttttgc tctgaccggc tgaatggcag acgtgctcat ctttttctat
      361 aagtaataat cgaagtcttt ttctgaacgc acacgttgca gcacccgaaa aacatgcgcc
      421 ggtaccgcat gcggtgatag ctcaatatag ttccgctggc cttcccatag atagcggttt
      481 cccgtcagtt catcctccac ctggaaaggt tcatgcgcat gcaggccaag tctctccata
      541 tccagattga cccaccccga ctggacatag tttggatcga ggttcacagc aatcagcact
      601 gtattcgcgc catccatgct ctgtttgcta tagcaaatca gatgcggatt gtctgtctca
      661 tgaaatgtaa gcatgtggct ggtttgcaag gctgcatttt cacgcctggc ccggttcact
      721 ttttcgagaa cgggagcaat gctgtgcgga gcatccaggt cccattcgcg caattgatat
      781 ttttctgagt tcagatattc ttcgctgccc tgccgaaaag gagcgttttc cagcaactca
      841 aaggcagggc catagatgcc gtaattcgta cccagcgtgg ccgccagcac cagtcgtgca
      901 ataaatgccg gccttccgcc ggtttgcaga tgttccggca gaatatcagg cgtgttcggc
      961 cacaggtttg gacgaaaata ctcacgcaat tctgaatgcg taagctgctg gagatattcc
     1021 gtcaattctt ctttcgtgtt gcgccatgtg aagtaggtat atgactgcga aaatcccagc
     1081 ttggccagcc gctccattac gcgcggacgc gtaaaagctt ccgacagaaa cagcacatcc
     1141 ggatattctg atttgatttc gtcaatcacc cactcccaga aagcaaatgc cttggtgtgt
     1201 ggattgtcta cacggaagat gcgcaccccc tgctcaatcc agaaaagaaa gacgtgcttc
     1261 agttcatccc aaagaccgct ccagtctttg ctttcaaaat tcagcggata aatgtcctga
     1321 tacttcttgg gaggattctc cgcgtactgg atcgaaccat cggcgcgctt gcgaaaccac
     1381 tccggatgtt ctttcaccca gggatggtcc ggcgaacact gaaaggcaat gtcaagagca
     1441 atctccagcc catgctcatt tgctttcttt acaagttgcc ggaaatcttc caatgtcccg
     1501 agttccggaa ggaccgcggt atgcccgcct ttttccgagc cgattgccca gggactcccg
     1561 acatcaccgg gctctgcatg gactgtattg ttttttcctt tgcggaagct gcgtccaatc
     1621 ggatgaatcg gcggcatgta caaaacatca aaccccaacc tcgaaacata atcaagccgg
     1681 gcctcaacat cagcaaaggt gccgtgcttt ccgggcacag gcgagcacga acgcggaaac
     1741 aactcatacc agcttgaaaa ccgcgccttt tcgcggtcta cgacgatgct taattctctt
     1801 tcatactttg tttccagcga tgtgtccgga taccgctcca tcgcggcggc cagcgccgga
     1861 tcgagggccg ttgtacgggc ctcttcgctg tcgctcctgg ttttcagtat ctgtgcccac
     1921 tcttttaact gcgcggcgtc cttgccgcgc gcacgggttg ctgcctgctc taccatggtg
     1981 gcgccggttt gcagttccag tgttacgtct tgcgctgcct caagacgttt gatcaaatcg
     2041 cttcgccatg tatcgaaatg gtctatgcga cctgcaatgg tatagacgta gcggccaatt
     2101 tttgtcaccg taaatgaagc gcgccagcgg tcgttgccca acgcttccat cttcgcgctc
     2161 gaccaattcc gctcatcttc atgacgaaaa agcagccggc aagccacgtg atcatgacca
     2221 tcgccaaaga catcgccctc tacatggaca gtatcgccaa caatgcgttt gatggggaat
     2281 cggcctgcat ccagttcagg cgagacatgc tctataacga cacgctttct accgtccttt
     2341 ggttttacca tgcacgttcc ggttttgacg cagaaattct tctatcagac agatgcaaat
     2401 tcaatagaat gcgttcgtta ggggagagag aattttatat ttattcatga gtaagcgcgc
     2461 ggatgatgtc cgcagcgcct gggaactgcg caatcaattc attccgcctc ccggcctcgt
     2521 aatcggcata gtcaaaccca gggctgaccg tgcatccaag aagcgcaaat tttcctccgg
     2581 cacgcagccg cgaaccctgc cacacaccct tccggaccat cgcctgtacc tgcatcccgg
     2641 cagcaagatc gtggcctaga atgaccgtct gcgttgaacc atccggccat agctgcaaca
     2701 tctccaccgg gtcaccgaga tagaagtgaa atatctcgtc agaagccagc cggtgcactt
     2761 cagaaaaagt atgcggctca agcaaatagt agatggccgt tcctgctgcg cgctccgacg
     2821 aatatcgcgt tggcagggac acctttggga ttatttcctc cgcgcgccat gtttcagcaa
     2881 accatccgcc ctcacaggga tggggcttca gatgcagcag acttttcaaa tcatcagcag
     2941 tcataatcat gtccggatta tggtttcacg gccgaaagaa attttcgcgc cacttctccc
     3001 accctctcat cgggtccgaa cagggcagaa atcacagcca ccgaatctgc tccggcatct
     3061 atgatctgac gcgcattttc cagtgtgatg cctccaatcg caaccagagg ctttgctgtc
     3121 agctttcggg cctcgcgtac tccttccaga ccgatcaccg gatcaggatt ggccttcgta
     3181 gaagtagcaa aaacaggccc tatcgcgatg tagtccacag gctgtgtgtt ggcaatttca
     3241 atctgcgcgc ggttgtgagt cgaaacgccg agaatccggt ctggccctaa aatctttcgc
     3301 gcttcttctg gattcatgtc ttgctgcccc acgtgtgccc catcaaaacc agcacgcact
     3361 gccagcccag ggcggtcatt catcatcagc cttacgcgcc cgccagcgga ctggtacatc
     3421 acagcagcat ccttgagaat ctctgcttct tcgctctgct tattgcgata ttgcagcaga
     3481 gaaacgccgg catcagccaa ttctgtaacc aactgcctga gcctttcttc ccggtttcca
     3541 gaaggcaaaa atgaagtgtc caggatggga taaaaagaag gaatcgaaag aggcatgatc
     3601 gctcgttttt gtgccacaaa atgaaaaggt gcagaagagt tcacatcctt ttgcaccttt
     3661 ataacttgtt tattgaaaac tattaagccc gtaaattctt ttggaatcta tggcggggac
     3721 gacggggctc gaacccgcga cctctgccgt gacagggcag cattctaacc aactgaacta
     3781 cgtccccagc ttttcagcga cttgccggaa tccggctgtc tcttctgaat ataactgatt
     3841 tcccattgaa atggcagaaa agaccctaat ttatcttgtc tttcccatgc gattgcaaat
     3901 acgaaagttg tgaatgaatc gctccttcat tgtaacaaaa tacatcctgt cttgttgtct
     3961 cgcctctctc actaagcctt gcgccgccgc tcatctctgc cggaaagata ttccggatac
     4021 acatgcgaaa gctctccggt gactgctgta acttcaggca cgaaatgact ccatacgtcc
     4081 tgatgccgtt ctgttatgct tcaggattaa tatattctgc aaagaagtga aggatttatg
     4141 gcaaagagcg tcaacaaagt gattcttctt ggaaatgtag gcaaggaccc tgagattcgc
     4201 gccacgccca atggcaccat tgttgccagt ttttcattgg cgacaactga ccgcacgaaa
     4261 gaccagagcg gcaactggac ggacaaaacc gagtggcata atctggttgc ttttcagcgc
     4321 acggccgaga ttattcgcga ttatgtgaag aagggctcca agctctacgt cgagggcaag
     4381 atccagaccc gttcatggga cgacaagaac agcggagaga agaagtaccg gacagaaatc
     4441 gttgtcaatg atctttctct gctgagcgga cgcggtgagg gcgaaaccgg cggttactcc
     4501 cgctcatcca atactgcgtc ttttaaccag agcgcacctg cgccggatga tctcagccag
     4561 gccgccgaaa tcagcgacga cgatattccc ttctaagcga actgcctgca tggaggagtg
     4621 cattgcctga attgccgagt gtgcctatta cgctggaagg ctcaagcgtt ctgcaccaga
     4681 tgttccattt tgactggagt tcctggaaga aactcccatc tgaagagaag caggcggtca
     4741 tcagcgaagc tgtggggctt tttgcgcggt gggaacagga cagtgcgggc cagtccgctg
     4801 cgttttctct tctggggcat aaaggagatc tgctgctggt acacttccgc aaatcctttg
     4861 cggacctcgg acgtgcccag cttggccttg ctcaaaccaa gctgcacgat ttcctgaaag
     4921 cctctcattc ttatctttcg gtcgtagagc tggggcttta cgagtcttcc gccaaaacct
     4981 atgcagcgct ggccgaacaa ggtctggaac ccggtactcc ggaatgggat gcaggtgtgg
     5041 gcgaagtggt cgagcgccaa tcgaaagcga tggcgcagcg gctatatcct gcgattccgg
     5101 atgcaaaata catctgcttt tatcccatgg accgcaagcg cggcgagcag gtgaactggt
     5161 acaccgaacc gatggccgac cgtcagagca tgatgcatga acatggcatg atcggacgcc
     5221 gttacgccgg acaggtccgc cagatcatta ccggatccat tggatttgat gactgggaat
     5281 ggggcgttga tcttttcgcg aatgatccgc ttgtcttcaa aaagctgatt tacgagatgc
     5341 ggttcgaccg ggt
//
LOCUS       DASRBV010000341         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_592714, whole genome shotgun sequence.
ACCESSION   DASRBV010000341 DASRBV010000000
VERSION     DASRBV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592714"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..376
                     /locus_tag="VFA02_12765"
     CDS             59..376
                     /locus_tag="VFA02_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10767.1"
                     /translation="MKFSLAHATMLAAAALLFVTGCETGRTHKLHATWQAVSDETTTG
                     KPQMASAAPATGQPPVPGSPTAAGVNGLQPSRDDEARPSPGAEEGKPIPFNRQKKDPF
                     ERQ"
     gene            457..924
                     /gene="moaC"
                     /locus_tag="VFA02_12770"
     CDS             457..924
                     /gene="moaC"
                     /locus_tag="VFA02_12770"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZU10768.1"
                     /translation="MTKLSHFDESGEARMVDVSGKSPTRREAEASAFVALSPEVLTAL
                     PHNPKGNPLEIARFAGIQAAKKTSDLIPMCHPLPLSFVDVKAITTATGIAIRSTAATT
                     ASTGVEMEALTAASVAALTIYDMCKALDKGIVIEHVRLERKSGGKSGDYQRGT"
     gene            1053..1580
                     /gene="sixA"
                     /locus_tag="VFA02_12775"
     CDS             1053..1580
                     /gene="sixA"
                     /locus_tag="VFA02_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZU10769.1"
                     /translation="MNLYILRHASAGTRRKNPLIDVKRPLDKEGKQQCILVAAYLNAL
                     DVQFDLIASSPLKRALQTASLVGTELGYDQKINVTDALSPEANVAKFQEFVASLQKHE
                     NVLLVGHNPNLPQFLGSLIASPGRASIRLRKGAIARVDYTRRPGTLNWLVDPRILRGV
                     YTRVTKRSLRKTSLK"
     gene            complement(1532..3061)
                     /gene="ppx"
                     /locus_tag="VFA02_12780"
     CDS             complement(1532..3061)
                     /gene="ppx"
                     /locus_tag="VFA02_12780"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZU10770.1"
                     /translation="MQTFAAIDIGSNSCRLKIARVVQHRLKVLHEDREVTRLGGSVFE
                     TGLVSPEAMAATLGALKRFQRAVQMHGADKVRAVATAAMRDARNARAFLAWVRDETGW
                     EVEIISGLEEGRLIHRGIMSNEPGTSGRCLLIDVGGGSCEISLSEHKRIKETISVPLG
                     AVRLTQEFLHSDPPTKQDIARLKQFIARELRRAERRVQPTRVQTVIATSGTAAALSEA
                     SRTIGKQRAGTKSAITSTQSVRKLTEKLTKMNNAERMSVQGIGPRRSEIIVAGAHVFA
                     ELLEAYGLPGFRYSPMGLRDGILAQMLAAQDPRASVHQQFERERWEAVLETCRKYGVD
                     TRQAEPVRHHAAQLFHDLKPVHKLPDEYLIWLEAAAMLRDIGKFMNYQGYHRHAQYII
                     ANSEMFGFTPAQRVITSAIARYLGKSRPEPTGRTMRQVPPEEHEHVRRAVVLLRLAVA
                     LNQDRASDVLRVRVRVYPKQVLLEIFPGRTGAELEMWSLRKEAAYFKEVFRKDLFVTL
                     V"
     gene            complement(3127..4362)
                     /locus_tag="VFA02_12785"
     CDS             complement(3127..4362)
                     /locus_tag="VFA02_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU10771.1"
                     /translation="MKRVRYTKYDGSLADEMSMEDLLNALSDFLLDSGFQNPWTQFQD
                     LDQTMEALREALRQALESGELFNEQMQQKIDELAAQNKLDELIDQIIQRMEQENYISV
                     EQPRESMQTPSSTGQIGENESQARFEVTDKSLDFLGYKTLRDLLGSLGKSSFGRHDTR
                     DWATGIEASGASRPYEFGDTLNLDVVTTLSSAIAREGLSLPLNLEYNDLHVHQCEYQS
                     SCATVVMLDCSHSMILYGEDRFTPAKRVAMALSHLIRTQYPGDSLSLVLFHDSAEEMP
                     VSQLARVKVGPYYTNTREGLRLAHRILDRQRKDMKQIVMITDGKPSALTLPDGRIYKN
                     AFGLDPLVVSETLEEVSRCKRSGIMINTFMLASDFGLVQFVQRVTAMCKGKAYFTTPH
                     TLGEYLLMDYMQRKMKTIH"
     gene            4544..>5730
                     /gene="xylB"
                     /locus_tag="VFA02_12790"
     CDS             4544..>5730
                     /gene="xylB"
                     /locus_tag="VFA02_12790"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZU10772.1"
                     /translation="MAFLGMDVGTGGTRAVLVDEQGHVIASGVSEHAPFRSPQPGWAE
                     QDPEDWWRAAQEAIHAALNASGLKSSDIAAIGLTGQMHGAVMLDKEGRVLRPSLIWCD
                     QRTGPQCDWLHEKIGRERLIELTCNPALPNFTLTKLLWVRDHEPEVFARIAHVLCPKD
                     YVRYRLTGTFAIDVQEASGTLLLDVAHRRWSSEVARISGINEAWLPQVFESPEVCAKI
                     SPEAAKLTGLLAGTPVVAGAGDQGAGAVGMGILQPGSVSATIGTSGVVFAATDAPTKD
                     PQGRLHTFCHAVPGRWHVMGVTQAAGLSFRWLRDTIAPGASYDTLTAEASQVPAGSDG
                     LLWAPYLFGERTPHLDSYATAMFYGITATHTRGHLTRAVLEGVAYSLKDTFTLFSELG
                     IPV"
BASE COUNT         1219 a         1713 c         1586 g         1212 t
ORIGIN      
        1 gcagcgtatt gaatctgtga aaatgccgta aaaaatgccc gcaggaaagg acatctgaat
       61 gaagttctct ctcgcccatg caacgatgct ggccgcagca gcccttctgt ttgtcaccgg
      121 ttgcgaaaca ggacgcaccc ataaacttca cgccacatgg caggccgtat cggacgagac
      181 cacaaccgga aaaccccaga tggcaagcgc agcgcctgct acggggcagc cgcctgttcc
      241 cggctctcca accgccgccg gagtgaatgg actccagccg tccagagacg atgaggcccg
      301 ccccagcccc ggcgctgaag aaggcaaacc cattcccttc aaccgccaga agaaagaccc
      361 attcgagcgc cagtaatcag ttggccatcc cgggtgccca tccttttgcg tttcagtgca
      421 caaaaggatg ggaaaattac ggtcttaaaa tgttctatga ccaaactctc gcactttgat
      481 gaatccggcg aagcccggat ggtggatgta agcggaaaat cccccacgcg ccgcgaagct
      541 gaagcctccg cattcgtcgc gctttcgcct gaagtattga cggcactacc acacaacccc
      601 aaaggcaacc ctcttgaaat tgcacgtttc gccggaatcc aggcggccaa aaagacgtcg
      661 gatcttattc cgatgtgcca ccctctgccg ctcagttttg tggatgtaaa ggcaataaca
      721 acggccaccg gaatcgctat ccgcagcacc gctgcaacca ctgcgtccac cggggtcgaa
      781 atggaggcgc tcaccgccgc ctctgtggcc gcgcttacga tctatgacat gtgcaaagcg
      841 ctggacaaag gcatcgtgat cgaacacgtg cgtctggagc gaaaatccgg cggaaaaagt
      901 ggcgattatc aacgtggaac ctgaggacct ttcctgccat ctgacctcca gcaaaacctt
      961 ttatctctgc ataagcttca tagactgcga attgttacaa tggtgttaat tcctggggtt
     1021 tagtttttgc ccgtccagaa agaatttttc tcatgaacct ttacattctg cgtcatgcca
     1081 gcgctggaac acggcgcaag aatcctctga ttgatgtaaa aaggccgctc gacaaggaag
     1141 gcaagcagca atgcattttg gtggccgcct atctgaatgc gctcgatgtg cagtttgact
     1201 tgattgcctc cagcccattg aagcgcgcgc tccagacagc ttcattagtt ggaacagaac
     1261 tgggctatga ccagaagatt aatgtgaccg acgcgctttc tcccgaagca aatgtcgcaa
     1321 aattccagga attcgttgcc agcctgcaga agcacgaaaa cgtcctgctg gtaggacaca
     1381 accccaacct gcctcagttt cttggctcgc tcatcgcctc acccggccgc gccagcattc
     1441 gtctgcgcaa gggcgcgatt gcaagagtgg attacacgcg ccgcccgggt acgctcaact
     1501 ggctggtgga tccgcgcatc cttcgcggcg tttataccag agtcacaaag aggtctttgc
     1561 ggaagacttc tttaaaataa gcagcctctt ttcgcagcga ccacatctcc agttcagccc
     1621 ctgtgcgccc gggaaagatc tccagcaaaa cctgttttgg ataaacgcgc acccggacac
     1681 gcaagacatc gctggcgcgg tcctggttca aggcgaccgc cagacgcagc aagaccacgg
     1741 cccggcgcac atgctcgtgc tcttccggag ggacctgtcg catggtgcgc cctgtaggct
     1801 cgggacggct cttgccaaga tagcgcgcga tggcggaagt aatcacgcgc tgtgcaggcg
     1861 taaagccgaa catttctgaa ttggcaatga tgtactgcgc gtgccggtga tatccctggt
     1921 aattcatgaa tttgccgatg tcacgaagca tggcggcagc ttccagccag atcaggtatt
     1981 catctggcag cttgtggacc ggcttcaggt catgaaacaa ctgtgcggca tggtggcgaa
     2041 caggctctgc ctgacgggtg tctaccccat acttgcggca ggtttccagc accgcctccc
     2101 accgctcgcg ctcaaattgc tgatgcacag aggcacgcgg gtcctgtgcg gccagcatct
     2161 gcgcaagaat gccatcgcgc aggcccatcg gtgaatagcg aaaacccggc agcccatagg
     2221 cctccagcag ctctgcaaaa acatgcgcac cggcaacgat gatctctgaa cggcgcggcc
     2281 caatgccctg gacgctcatc cgctcggcat tgttcatctt tgtaagcttt tctgtcagct
     2341 tgcggacgct ctgcgtagat gtgatcgcgc ttttcgtgcc cgcgcgttgc tttccgatcg
     2401 tacggctggc ttccgaaagc gccgcggcag tgccggaagt ggcaatcacg gtctgcacgc
     2461 gtgtgggttg tacgcggcgc tccgcccggc gaagctcacg ggcaataaat tgcttgagcc
     2521 gggcaatgtc ctgctttgtc ggcggatcgc tatgcagaaa ctcctgcgta agccgcactg
     2581 cccccagcgg aacactgatt gtctctttga tccgtttgtg ctcggagagt gaaatctcgc
     2641 agctcccacc gcctacgtct atcagcaggc aacggcctga tgttcctggc tcgttactca
     2701 taatgccgcg atgaatcaga cggccttctt ccaggccgga gatgatctcg acctcccatc
     2761 cggtctcatc gcgtacccat gcaagaaaag cgcgggcatt gcgggcatcg cgcatcgctg
     2821 ccgtcgctac ggcgcggacc ttgtcggctc catgcatctg cacggcccgc tgaaagcgct
     2881 tgagtgcgcc aagtgttgcc gccatagctt cgggagatac cagtcccgtc tcaaacacgc
     2941 tgccgcccag gcgggtcact tcgcggtctt catgcaggac tttcagacgg tgttgaacaa
     3001 cacgcgcaat cttcaggcgg caggaattgg aaccaatatc aatggcggcg aatgtctgca
     3061 tagttcaggg gtgcttttcc ctgaaagaaa gatacacgag tgaacagaca aattttcaga
     3121 gattcgctaa tggatcgtct tcatcttgcg ctgcatgtag tccatcagca ggtattcgcc
     3181 caatgtatga ggcgtggtga agtaggcctt tcccttgcac atggccgtga cacgctgcac
     3241 aaactgcacc agtccgaaat ctgaggcaag catgaaggtg ttgatcataa tgccggagcg
     3301 tttgcagcgt gagacctctt ccagcgtttc gctcaccacc agcggatcaa gcccgaacgc
     3361 atttttgtaa atgcggccgt cgggaagcgt aagcgcagaa ggttttccat cggtaatcat
     3421 gacaatctgc ttcatgtcct tgcgctggcg gtcaaggatg cggtgcgcca ggcgcagccc
     3481 ctcacgcgtg ttggtgtaat acgggccgac cttcactcgc gccagttgcg aaaccggcat
     3541 ctcttccgct gaatcgtgaa agagcaccag cgacagcgag tctcccggat attgcgttcg
     3601 gatgaggtgc gaaagcgcca tcgcaacgcg cttggccggg gtgaagcggt cttctccgta
     3661 cagaatcatc gaatgcgagc agtccagcat gaccacagtc gcgcaggagc tttggtactc
     3721 gcactggtgc acgtgcaggt cgttgtactc aagattcagc ggcaatgaca gcccctcgcg
     3781 ggcaatggca gaactgagcg tagtaaccac atcgagattc agcgtgtcgc caaactcgta
     3841 agggcgcgat gctccgctgg cttcgatacc tgtcgcccag tcgcgcgtgt catggcgtcc
     3901 aaaactcgac ttgcccaggg agccaagcag gtcgcgcagc gtcttatagc ctaaaaaatc
     3961 gagactcttg tccgtcacct cgaatctggc ctgagattcg ttctcgccga tctgtccggt
     4021 agaagacggc gtctgcatgg actcgcgcgg ttgctccacc gaaatgtaat tctcctgctc
     4081 catgcgctgg ataatctggt caatgagttc atccagcttg ttctgtgctg ccagctcatc
     4141 aatcttttgc tgcatctgct cgttgaacag ctcgcccgat tcgagcgcct gccggagcgc
     4201 ctcgcgcagc gcttccatgg tctggtccag gtcctgaaac tgcgtccagg ggttctgaaa
     4261 gccggagtcc agcaaaaaat ccgaaagagc attcagcagg tcctccatgc tcatctcatc
     4321 tgccaggctg ccatcatatt tggtgtaccg gacgcgcttc atgcctttag tgtgcgcctg
     4381 aatacaaaag cgaagcaaat gtgtaaaaga ccgggtgtgg ggtgctcagt tgggcgcggc
     4441 cgacctggcc ttcaggattc ggggtggctg gaaatctttg tcaatccttg ccggaatgtt
     4501 acatttaatt ccgatactaa ttcgctttac ttaggatgaa gctgtggcat ttctggggat
     4561 ggatgtgggc acgggtggca cccgcgcggt gctggtagat gaacaggggc atgtgatcgc
     4621 atcgggcgtc agtgagcatg ctccctttcg cagccctcag ccaggttggg ccgagcagga
     4681 ccccgaagac tggtggcgcg cagcgcagga ggccatacat gctgctctca acgccagcgg
     4741 actgaagtcc tctgatatcg ccgccatcgg ccttaccgga caaatgcacg gcgccgtcat
     4801 gcttgacaaa gaaggccgcg tgcttcgccc gtcgctcatc tggtgcgacc agcgcaccgg
     4861 accacaatgc gactggctgc atgaaaaaat tggccgggaa cgcctcattg agttgacctg
     4921 caatcccgcg ctgccaaact ttacgctgac caagctgctg tgggtacgcg accacgagcc
     4981 ggaggtcttc gcccgaattg cgcatgttct ctgcccgaag gactatgtcc gctaccgtct
     5041 gactgggaca tttgccatag acgtgcagga ggcatccggc actctgctgc tcgatgtggc
     5101 ccatcgccgc tggtcttctg aagtcgcgcg catttccggc ataaacgaag cgtggctgcc
     5161 gcaggtcttc gagtcgcccg aggtctgcgc aaagatttca cccgaagcag cgaaactgac
     5221 aggtcttctt gccggaactc cggtcgtcgc cggagcgggc gaccagggcg ctggcgcggt
     5281 cggcatgggc attctgcaac ccggttccgt ctcggccacg attggcactt caggcgtggt
     5341 cttcgcagcc accgacgctc ccacgaaaga tccgcaggga cgcctgcaca cattctgcca
     5401 tgctgtcccc ggccgctggc atgtgatggg agtgacccag gccgccgggc tttccttccg
     5461 ctggctgcgt gataccatcg cccccggcgc aagctacgac acactgaccg ctgaggcctc
     5521 tcaagtcccg gcaggcagcg atggcctgct gtgggcgcca tatcttttcg gtgagcgcac
     5581 cccgcatctc gattcctatg cgacggcgat gttctacggc atcacggcca cacatacgcg
     5641 cggacatctt acccgcgcag tgctggaagg cgtggcctat tcgctcaagg acaccttcac
     5701 gcttttctcc gaactgggta ttccggtaaa
//
LOCUS       DASRBV010000342         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_232245, whole genome shotgun sequence.
ACCESSION   DASRBV010000342 DASRBV010000000
VERSION     DASRBV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232245"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..363
                     /locus_tag="VFA02_12795"
     CDS             55..363
                     /locus_tag="VFA02_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZU10773.1"
                     /translation="MVRVILPQHLRTLAHVNGEVLLEVEGAVTLGLVLDMLEEKYPVL
                     RGTIRDHDTKQRRAFLRFFACEEDLSHEPPDTPLPEAVTNGTEPFIILGAVAGGTAVP
                     "
     gene            441..833
                     /locus_tag="VFA02_12800"
     CDS             441..833
                     /locus_tag="VFA02_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10774.1"
                     /translation="MKVVVIIARILLGLMFFVFGLNGFLHFIPMPPMPPSDATTFSLI
                     LMRSHYIYLISGTQVVAGFLLLIGQYVPFAEVLLAAMLANIFAFHIALQHGSGIGMGV
                     IALVLWLIVAWGYRVQLLPILARKPGVE"
     gene            complement(927..>2075)
                     /locus_tag="VFA02_12805"
     CDS             complement(927..>2075)
                     /locus_tag="VFA02_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU10775.1"
                     /translation="PFSRMKTYGGHGYTVAPTWALAYPKEIFPEMLKAVPSMTQRCVS
                     VLLDRVREVTRMEQQTEKLNALGKLAGNLAHELNNPASAAQRAASGLLEELHVYGYEK
                     YRLGNLCLSEDHITQVRAWQQSVREHAKNDGHSNPAEYAAREDALLAWMREHNVESPW
                     KIAPELAEAGVEPLQIEPLTDFLDAHALTVVLSQFASSLRAESMANAMLDSTGRIFDL
                     IRAIKDYSYMDQAPIQEVDIPQGLENTLTMLQSRLQHVEVERRYAPDLPRISAYASEL
                     NQVWMALLENALDAIQDSGRITLSVQPSGDMLLIEVWDNGPGIPPELQDRIFEPFFTT
                     KAPGSGLGLGLDTVQRIVRKHRGYVRVQSEPGATCFQVRLPVEQLQAY"
BASE COUNT          402 a          581 c          619 g          473 t
ORIGIN      
        1 acaagatcat cattgccgac ctgcagagtg cgtatcaggc ggcataggtt gagcatggtc
       61 cgtgtcattc ttccacagca tctgcgcacc cttgcgcatg tgaacggcga ggttttgctt
      121 gaggtcgaag gtgcggtaac gctgggcctt gttctggata tgctcgaaga gaagtatccg
      181 gtcctgcgcg ggaccattcg cgaccatgac acgaagcagc ggcgcgcctt tctacgcttc
      241 tttgcctgcg aagaagatct ttctcacgaa ccgcccgaca cccccctgcc cgaagcagtg
      301 acaaacggaa cagaaccctt cattattctg ggggctgttg ccggaggaac agcagttccg
      361 taaaatatgc gaaccagtga aaacagtgca gcatctgctg ttgtaacaac aattctgccg
      421 tgaatggagc gtaacagcca atgaaagttg tggtgatcat cgcgcgcatt cttctggggc
      481 tgatgttttt cgtcttcggc ctgaacgggt tcctgcactt cattcctatg ccgcccatgc
      541 ctccgagcga tgctacgacc ttttctctca ttctgatgag gtcgcattac atttatttaa
      601 tttcaggcac gcaggttgtg gcaggttttc tgctgctgat tggccaatat gtgccgttcg
      661 cggaggtgct gcttgccgct atgctggcaa acatctttgc gttccatatt gcattgcagc
      721 atggcagcgg tatcggaatg ggtgttattg cgctggtgct gtggttgatt gtggcgtggg
      781 ggtaccgggt gcagcttctg ccgatcttag caagaaaacc aggagtggag tgaagctgct
      841 gattcactca ttcggtggtg aacgtagtgc agctgggcga cctgtacccg aaacgggagc
      901 gggtctgccc tgagcgctgt gctctttcaa tatgcctgca actgctcaac tggcaggcgc
      961 acctggaagc aggttgcgcc tggctcagac tgaacgcgaa catatccgcg gtgcttgcgc
     1021 acgatgcgct gcaccgtatc gaggccgagg ccgaggccac tgcccggggc cttcgttgtg
     1081 aagaacggct cgaagatgcg gtcctgcaac tcgggtggaa tgcccgggcc gttgtcccat
     1141 acttcgatga gcagcatgtc tcccgagggc tggacgctga gcgtgatacg tccgctgtcc
     1201 tggattgcgt ccagcgcatt ttcgagcagc gccatccaga cctggttcag ctcgctggca
     1261 taagcactga tgcgcggcag gtccggagca tagcggcgct ccacctccac gtgctgcagg
     1321 cgcgactgca acatggtcag tgtgttttcc aggccctgtg gaatgtccac ctcctggatt
     1381 ggcgcctggt ccatatagga gtagtccttg atggcgcgga tcaggtcgaa gatgcgtccg
     1441 gtggaatcga gcatggcatt ggccatgctc tccgcgcgca gagacgaggc gaactgcgac
     1501 aaaaccacgg tcagtgcgtg ggcatccagg aaatccgtga gcggctcaat ctgcaatggc
     1561 tccacacccg cctcggccag ttccggagca atcttccacg gcgactccac attgtgctcg
     1621 cgcatccatg ccagcagggc gtcttcgcgg gccgcatatt cggcaggatt gctgtggcca
     1681 tcgttctttg cgtgctcgcg caccgattgc tgccaggcgc gtacctgggt aatgtggtcc
     1741 tcagaaagac aaagattgcc caggcggtac ttctcgtatc cgtagacgtg cagctcttcg
     1801 agcagaccgg aggcggcgcg ctgcgcagcc gacgcaggat tgttcagttc atgcgcgaga
     1861 tttccggcga gcttgcccag agcattcagc ttctctgtct gctgctccat gcgcgtgact
     1921 tcgcgcacac ggtccagcaa tacgctgaca caacgctggg tcatggacgg gacggccttc
     1981 agcatctccg gaaaaatctc tttgggatac gccagggccc aggtaggtgc caccgtgtaa
     2041 ccatgcccgc cgtaggtttt catacgggaa aaagg
//
LOCUS       DASRBV010000343         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_697231, whole genome shotgun sequence.
ACCESSION   DASRBV010000343 DASRBV010000000
VERSION     DASRBV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697231"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFA02_12810"
     CDS             <1..807
                     /locus_tag="VFA02_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZU10776.1"
                     /translation="MLLKTASFNQVRSQREALRRDYAQLEQIAHEKEVQAASLGSLAS
                     EISALYGLRQNKLTSKAAEAGPSPLTEASDNFSEQAYAQSLDQLYALRRTALSGQVYP
                     ASVNMRRPFSVSDDILNLANAPSLWPVVGPVTSSFGERQDPFNGEGAFHAGIDISSTF
                     GTPVRATADGTVEMADRASGYGREIVIDHGYGVKTVYGHLSGFAVTDGQEVTRGQIIG
                     YVGTSGRSTGPHLHYEVRIRNTPVNPHKYLRETMQQLASTGTQSTTGGMQ"
     gene            complement(910..2232)
                     /locus_tag="VFA02_12815"
     CDS             complement(910..2232)
                     /locus_tag="VFA02_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZU10777.1"
                     /translation="MGRGFYRCAVFTAAGAFLLSASISRADGPPSGKVLTGQTAFTDY
                     SKEKPGVRRKLTVADLPAPYATKGVDNGADMVPRPNNAWPQAPQGFKVGLYATGLNQP
                     RLIRTAPNGDLFVAMSYENKVLVFRGIGPDGKARQVETFADNLNMPFGIAFYPAGPNP
                     KWVYIGNTDSVVRIPYQTGDLKARGPAQKLADLPGGGRLRGGGHWTRDIAFSKDGTKM
                     FVSVGSHSNVDDPDTHPEEFHRADVLEFTPEGKFIKVYAYGIRNCVGEAINPITGELW
                     CSTNERDMLGDNLVPDYITHIQEGGFYGWPWWYMGPHQDPRHQGKHPELENKVITPDV
                     LLQPHFASLEMLFYEGNQFPAEYKGDAFAAEHGSWNRAHRAGYEVIRVPMKDGHATGE
                     YEDFLTGFTTPDGHVWGRPVGVTTGNDGSLYVTDDGSGSIWHITYTGK"
     gene            complement(2284..>3198)
                     /locus_tag="VFA02_12820"
     CDS             complement(2284..>3198)
                     /locus_tag="VFA02_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10778.1"
                     /translation="VYLRKKAPPEAARLLPESDGIVYINLRPLRAATHFDRHPVQHDP
                     DYQKFIDETGIQFERDLDEVAFALHRMPNPAGPNGPVAFSEVFVGHFDGRRLTKFLES
                     ESNSRENYAGHIIYSIPNDGRTVRVVLLGYDMVAVSNTPTAEQIHSILDRYRSAALPF
                     SGSTLLSDHYQDVPLLSLAWGIGQIGLPFGDSGDIHILGVRLPVTFDSTFVASLRWTG
                     ALHLRIEEIAPTEEAAAASAESVQTLVSLFRAAQPNFTAPDTAGWKPLLDSIRIDHHK
                     NRAVLRATLPDALLEKLLNPETLQTPQK"
BASE COUNT          625 a          845 c         1047 g          681 t
ORIGIN      
        1 atgcttttga agacggcaag cttcaatcag gtccgttcgc agcgcgaggc gcttcgcagg
       61 gattatgctc aactggagca gatagcgcac gaaaaggaag tccaggcggc ctcgctcggc
      121 tcgctggcaa gcgaaatcag cgcactttac ggactccgcc agaacaagct gacctcgaaa
      181 gcagcagaag caggcccttc tcctcttacg gaggcttctg acaacttcag cgaacaggca
      241 tacgcacaat ctctggacca gctttacgcg ctgcgccgca cggccctctc cgggcaggtg
      301 tacccggcga gcgtcaacat gcggcgtccg ttcagtgttt cagacgacat tctgaacctg
      361 gcgaacgctc cttccctgtg gccggtggtt gggcctgtga caagctcctt tggtgagcgt
      421 caggacccgt tcaatggtga aggcgcattc catgcaggta ttgatatttc gtccacattt
      481 ggaacacctg tgcgggccac ggccgatggg acggtggaga tggccgaccg cgcttccggt
      541 tacgggcgcg agattgtcat tgaccacggt tacggcgtca agacagttta cgggcacctt
      601 tccggctttg ccgtaacgga cggccaggaa gtgacccgag gccagattat cggctatgta
      661 ggaacgagtg gccgcagcac agggccgcat cttcactacg aagtgcgcat ccgcaatact
      721 cctgtcaatc cccacaaata tctgcgtgag accatgcagc agcttgccag cacggggacg
      781 cagtccacga ccggcggtat gcagtagatt tcccgggatt tgttccagcc tcacaccgct
      841 aagattcttc cttcctttgg ccgtcagaat gacggacttt ccatacacgg aaaaggctga
      901 tatcgcacat tactttccgg tgtaggttat gtgccagatg gagcctgagc cgtcgtcggt
      961 gacgtagagt gagccgtcat ttcctgttgt gactcctacc ggacggcccc agacgtgtcc
     1021 gtcgggggtg gtaaatccgg tgaggaagtc ctcatattct ccggtggcgt ggccatcttt
     1081 catagggacg cggatgactt cgtatccggc gcggtgggcg cggttccagg agccgtgttc
     1141 ggcggcgaag gcgtcgccct tgtactcagc cgggaactga ttgccctcgt agaagagcat
     1201 ttcgagcgag gcaaagtgcg gctgcaggag gacatccggc gtgatgacct tgttttctaa
     1261 ttcaggatgt ttgccctgat ggcgcggatc ctggtgcggg cccatgtacc accatggcca
     1321 tccatagaag ccgccttcct ggatgtgggt gatgtagtcg gggacgaggt tatcgccgag
     1381 catatcgcgc tcgtttgtgg agcaccagag ttctccggtg atggggttga tggcttcgcc
     1441 gacgcagttg cggatgccgt aggcgtagac cttgatgaat ttgccttcgg gagtaaattc
     1501 gagcacatcg gcacggtgaa attcttcggg gtgggtgtcg ggatcatcca cattggagtg
     1561 tgagccgacg gagacgaaca tctttgtgcc gtctttggag aaggcgatgt cgcgggtcca
     1621 gtgaccgccg ccacgcaggc gtccgccgcc gggcagatca gcgagtttct gtgccgggcc
     1681 gcgcgccttc aggtcgcctg tctggtaggg aatgcgcacg acggaatcgg tattgccgat
     1741 gtagacccat ttcggattgg ggccggcggg gtagaaggcg ataccaaagg gcatattcag
     1801 gttgtcggcg aaggtctcaa cctgtctggc cttgccgtcg gggccgattc cgcgaaagac
     1861 gaggacttta ttttcgtagc tcatggcgac gaacaaatcg ccgttcgggg cggtgcggat
     1921 gagacgtggc tgattgaggc cggtggcgta aagcccgacc ttgaaaccct ggggggcctg
     1981 tggccatgcg ttattgggcc gcggcaccat gtctgcgccg ttgtctacgc ctttggtggc
     2041 gtaaggagcc gggaggtcgg ccactgtgag cttgcgccgg acacctggtt tttccttgct
     2101 gtaatcagta aatgcagtct ggccggtgag gaccttaccg gacggcgggc catcggcgcg
     2161 ggagatagaa gcagacagca ggaacgcacc tgccgccgta aagactgcac agcgatagaa
     2221 acctcttccc atgatttcgg tcctccagtg gtcgtaccac tccattgtag ttttaaacga
     2281 ttttcatttt tgaggcgttt gcagcgtctc aggatttaaa agtttttcaa gcagcgcatc
     2341 gggcagggta gcacgcagga cggcgcggtt tttgtggtgg tcaatcctga tgctgtcgag
     2401 caggggcttc cagccggcgg tatctggcgc ggtgaagttt ggctgtgcgg cgcggaagag
     2461 actgaccagc gtctgcacgg actcggcgga tgcggcggct gcttcttccg tgggcgcaat
     2521 ctcttcaatg cgcagatgca gcgcgccggt ccagcgcagt gatgcaacga aagtggaatc
     2581 aaaggtgacg ggcaggcgca caccgaggat gtggatgtcg ccgctgtcgc cgaagggaag
     2641 gccgatctgg ccgatgcccc aggcgagcga gagcaggggg acgtcctgat agtggtcgct
     2701 taataaagta gagccagaga aaggaagggc cgcggagcga tagcggtcga ggatggaatg
     2761 aatctgctcg gcggtgggtg tattgctgac ggcgaccatg tcgtagccga gcagcacgac
     2821 gcggacggtg cggccatcgt tggggatgct gtagatgatg tgtccagcgt agttttcccg
     2881 ggaattggat tcgctttcaa gaaattttgt gagcctgcgc ccgtcgaaat ggcctacgaa
     2941 gacctcagag aaagcgacgg ggccattggg acctgccggg ttgggcatcc gatgcagcgc
     3001 gaaggccacc tcgtctaaat cgcgctcgaa ctggatgccg gtctcgtcaa taaatttctg
     3061 gtagtcgggg tcgtgctgca ccgggtgtct gtcaaaatgc gtggctgcgc gcagcgggcg
     3121 cagattgata tagacgatgc catcggattc gggcaggaga cgggcggcct ccggcggggc
     3181 cttcttgcgc agatacac
//
LOCUS       DASRBV010000344         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_85448,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000344 DASRBV010000000
VERSION     DASRBV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85448"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFA02_12825"
     CDS             complement(<1..446)
                     /locus_tag="VFA02_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10779.1"
                     /translation="MEKMTFRTVRMIALLGAAALPLGFSAIMLAQSAAEKALITKAQK
                     LEATGHPEMAAQTWEQVLLADPNNREALLGIAKTDMQLGKTAEAKRYLDRLRANGESQ
                     EEVTKILETPRIQSRSGRLDEAGRFAQEGNYTEAMRVYREVFGDKPP"
     gene            complement(434..1573)
                     /gene="bcsZ"
                     /locus_tag="VFA02_12830"
     CDS             complement(434..1573)
                     /gene="bcsZ"
                     /locus_tag="VFA02_12830"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HZU10780.1"
                     /translation="MRQGILTAAVAMFSCFSFVAHSQSAWPFWNQYATSFISPQGRVI
                     DPDRNAITTSEGQSYALFFALVANDASTFDRLLSWTEKNLANGDIGKQLPAWNWGTKQ
                     DGAMGVLDSNSAADADLWIAYTLFEAGRLWKNPAYTQKGRALLAQIAQQEVTVVPGVG
                     TVLLPGREGFHPDKDTWFLNPSYFPLPVLLGMAHDDPQGPWKRMAEALPIWLKEASPA
                     GFAMDWVKCDAKGVSPAAQPGSSSEAAHGSYDAIRVYLWTGLAAREMPGQAQLLTALA
                     PMAKYVKTNGAPPESVAPDGHILSTAAPTGFLAATFPFLLAAGDVSTAEALLRNVKAQ
                     ILQSSGLLGTPARYYDQNLALFALGWYEKRFRFAPDGTLRPAWKK"
     gene            complement(1570..>3205)
                     /locus_tag="VFA02_12835"
     CDS             complement(1570..>3205)
                     /locus_tag="VFA02_12835"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HZU10781.1"
                     /translation="FAFASSVADPAVQQAAGIVASWFGVTAKSRPLSFPVSTGDILPK
                     GNVVLFADSFSDLPPGLGVSGEGAVVAVRTNPNDPYGKVLVLVGNNGEQLVAAARALA
                     LGNSLLQGKTARITAFQLPAPREADDAPLWLKTGTTASLWDYAGNTDLQSDGSGPLAV
                     YLRVPPDLYYGDRQDLPLHVDYRYNAISLANASTLRITANGGLVNELPLPHENNPKKT
                     LSYDAAVPLVNMRPFANTFLFNFYFQIAKMGNCQDTPPINLQGSILRSSYLDLRGLQH
                     WAAMPNLELFANAGFPFTRFADLSQTRIILPSQPSQQEMEVYLTLLAYFGEQTGYPAL
                     RVQTGDASKLGGNEDYLVLGTAADEPAFARLNGQLPVRIQEDGLTVRDTGGLFAAIKR
                     AWWQVAEMRPDWWWKLGRFDRDGLIESLGQLPDALLQGIESPWQSRRSVITITFRDND
                     AIAPFIAAFWKTSMSGDIGESVSVLHEKQFSSYRLGERYYHVGDLPLWVRIRYWLRTF
                     PWLIVVLTFVLGVFIVPWTKLWLDRRAQARLEAKNV"
BASE COUNT          687 a          988 c          879 g          651 t
ORIGIN      
        1 ggaggtttgt ctccaaagac ctcgcggtaa acgcgcatgg cctcggtgta atttccctcc
       61 tgggcgaagc gtcctgcttc atccagacgc ccggagcgcg actggatgcg aggcgtttcc
      121 aaaatttttg tgacttcctc ctggctttcc ccgttggcgc gcagccggtc cagataccgc
      181 ttcgcttcgg cggttttgcc gagctgcata tcagtctttg cgatgccgag cagcgcctcg
      241 cgattgttcg ggtcggcaag aagcacctgt tcccacgtct gcgcagccat ttccggatgc
      301 ccggtggcct ccagtttctg cgctttcgtg atcagggcct tttcagcggc agactgcgcc
      361 agcatgattg cagaaaaacc cagcggcagc gcggccgcgc ccaacagtgc gatcatccgg
      421 actgtgcgga aggtcatttt ttccatgcgg gcctcagtgt cccgtccggt gcaaaccgga
      481 agcgtttttc ataccagcca agcgcaaaca gcgccaggtt ctggtcgtaa tatcgcgcag
      541 gagttcccag cagaccagag gactgcaata tttgcgcctt cacattgcgc agcagtgcct
      601 ccgctgtaga tacatctccc gcagcaagca gaaaggggaa cgtagcagcc agaaaacccg
      661 ttggggcggc tgtgcttaaa atatggccgt cgggcgcgac gctttccggt ggcgctccat
      721 tggtcttgac gtactttgcc atgggagcaa gggccgtcag caactgcgcc tgcccgggca
      781 tttctctggc ggccagccct gtccagaggt aaacgcggat ggcatcataa ctgccgtgcg
      841 cagcctctga ggagcttcct ggctgggctg ccggagaaac accttttgca tcgcatttta
      901 cccaatccat agcaaatccg gcaggactgg cttccttcag ccagatcggc agggcttcag
      961 ccatgcgctt ccagggcccc tgcggatcat catgcgccat ccccagaagg accgggagcg
     1021 ggaaataact tggattcagg aaccatgtgt ccttgtctgg atggaagcct tctctcccgg
     1081 gaagcaggac tgtcccaaca cctggtacga cggtcacttc ctgttgcgca atctgtgcca
     1141 gcagtgctct tcccttttgc gtgtaagcag gatttttcca gagccttccg gcttcaaaaa
     1201 gcgtataggc gatccacaaa tccgcatcgg ctgctgaatt ggaatccagc acgcccatcg
     1261 ctccgtcctg ttttgtgccc cagttccatg ccggaagctg ctttccaata tcaccgttgg
     1321 ccaggttctt ttccgtccag gaaagcagcc tgtcaaaggt ggaagcgtca ttggctacaa
     1381 gcgcaaagaa cagagcgtag ctctgccctt ctgaagtcgt aatggcgttg cggtccggat
     1441 ctatgacccg tccctgcggg ctgatgaagc tggtcgcgta ctggttccag aacggccatg
     1501 cgctctgtga atgggccacg aaagagaagc aggaaaacat ggccaccgct gctgtaagaa
     1561 ttccctgcct catacgttct tcgcctcaag ccgcgcctgt gccctgcggt ccagccatag
     1621 cttggtccac ggcacaatga atacgccaag tacgaatgtc agaacaacaa tgagccaggg
     1681 aaaggtcctc agccagtacc ggatgcggac ccacaatggc aaatcaccca cgtggtagta
     1741 acgctcgccc agccggtagg aactgaactg cttttcatgc agcacactca ctgattcgcc
     1801 gatgtcgccc gacatcgagg tcttccagaa agctgctatg aaaggcgcaa tcgcgtcatt
     1861 gtccctgaaa gttatcgtga tgactgaccg gcgcgactgc cacggcgatt caatcccctg
     1921 cagcagagca tcgggaagct ggcccaggct ttcaatcagg ccatcgcgat cgaaacggcc
     1981 cagcttccac caccagtccg gacgcatttc cgccacctgc caccaggcgc gcttgatggc
     2041 tgcaaaaaga cctcccgtgt cgcgcaccgt cagaccatcc tcctgaatgc ggaccggaag
     2101 ctgcccattc aggcgcgcga aagccggctc atcggcggca gttcccaaaa caagatagtc
     2161 ctcgttgccg cccagtttcg aggcatcgcc agtctggacg cgcagcgccg gatatccggt
     2221 ctgctcaccg aaatacgcca gcagcgtaag gtacacttcc atctcctgct ggcttggctg
     2281 cgacggcaga ataattctgg tctgcgacag atcggcaaag cgggtgaaag gaaatccggc
     2341 attggcgaaa agctcgaggt tcggcatcgc cgcccagtgc tgcaaccctc tcaggtccag
     2401 ataagaactg cgcagaatcg agccctgcag attgatgggt ggggtgtcct ggcaattacc
     2461 catcttggca atctggaagt aaaagttaaa cagaaatgta ttcgcaaagg gacgcatgtt
     2521 gaccagcgga acagccgcat cataggaaag cgtcttcttc ggattgttct catgcggcag
     2581 tggaagctcg ttcaccagcc cgccattggc cgtaatacgc agcgtcgaag cgttggccag
     2641 agaaatagcg ttatagcggt agtccacatg caacggaagg tcctgtctgt ccccgtaata
     2701 aagatccgga ggaacacgca gatagactgc cagcggcccc gaaccgtcgc tttgcaaatc
     2761 ggtgtttccg gcatagtccc acagagaagc agtcgttccc gtcttcagcc acaagggcgc
     2821 gtcatcagcc tcgcgaggcg ctggaagttg aaacgcagta atcctcgccg tcttgccctg
     2881 gagcagcgag ttgcccaacg ccagcgctcg cgcggccgcc accagttgct cgccattgtt
     2941 gcctaccagc accagcacct tgccatacgg atcgttcggg ttcgtccgta ccgccaccac
     3001 cgcgccttcc ccgcttacgc ccagccccgg aggcagatcg gaaaagctgt cggcaaacag
     3061 cactacattg cccttcggca gaatgtctcc cgtgcttacc gggaaactca gcggcctcga
     3121 ttttgccgtc actccgaacc atgaggccac gatcccggcg gcctgctgca cagccgggtc
     3181 tgccacagaa gaagcaaagg caaag
//
LOCUS       DASRBV010000345        13415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_85667,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000345 DASRBV010000000
VERSION     DASRBV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85667"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFA02_12840"
     CDS             <1..279
                     /locus_tag="VFA02_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZU10782.1"
                     /translation="LHPPRKPDELRTGHFPSLRTPALFVHGTRDVFGSIAELANALRL
                     IPAAVELFPVESAGHELLTRHNRYALPLKIVETFRRFTGMACAGSTPN"
     gene            396..3071
                     /gene="aceE"
                     /locus_tag="VFA02_12845"
     CDS             396..3071
                     /gene="aceE"
                     /locus_tag="VFA02_12845"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZU10783.1"
                     /translation="MATKLASPAQADILEEVAEWIEAFDQVVVDEGPEQATELLNALR
                     QRAREAGINPPGEIRTPYLNTIPKHDEVPYPGDRQMERRVEALIRWNAMAMVHAQNKK
                     DAGIGGHISTYSSLATLLEVGFNHFFHASYGNQPGDFVYFQGHASPGVYARAYLEGRL
                     NDDHLKNFRHELRDKPGLSSYPHPWLMPEFWRFPTVSMGIGPLNAIYQARFMRYLENR
                     GLIEKTPRKVWAFVGDGETDEVDTLGAITVASREKLDNLIFVINCNLQRLDGPVRGNK
                     RIIDELEGVFRGAGWNVIKVIWGSDWDTLFERDHKGLLLKRMEECVDGEYQAFKAKGG
                     AYVRQEFFGKYPELLELVSDMTDEQLASLHRGGHDPVKIYNAYKRAVEHRGGPTVILA
                     KTVKGYGLGTTEARNAAHQEKKLTDESLTTFVKRFDIPVPEEAAKHGTPYKPADNAPE
                     LQYMHERRKALGGYMPKREVPAIHFKAPELDYFGEWTAGSKGRAVSTTMGFVSILRHL
                     MKDPEFGKLVVPIVPDEGRTFGLESAIRQVGIYTPEGQKYKPHDLDMLLYYREEKDGQ
                     ILEEGITEAGSMASFTAAGTAYANYRVPTIPFYMYYSMFGFQRVGDMIWAFADSRGKG
                     FLMAGTAGRTTMLGEGLQHQDGHSHVLASTVPTCLSYDPAFVYELAVILQDGLRRMYQ
                     ENEQVFYYITMYNEDYAMPEMPQTEGVREGIVRGIYKYKSAIKGKADAQLFGSGPILN
                     EALRAQQILAEKYGVQTDVWSVTSYNELRREALSVERWNRLHPAEPEKTPYVLQAIKG
                     AKGPIIAATDYMKSVPDQLAPWLKDRLVTLGTDGFGRSDNREYLRRHFEINAESIVAA
                     TLSKLARDGKFDAKRAQKAFEELGLDTEKVDAAKA"
     gene            complement(3118..3609)
                     /locus_tag="VFA02_12850"
     CDS             complement(3118..3609)
                     /locus_tag="VFA02_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10784.1"
                     /translation="MRHLAQIKIHRAAADEMEAAWDIVCEYYEAVGVLAREDRAGFER
                     EYFGNGAGIWLALSESDEAIGCIALRRLPRLKKAGEIKRMYVRPEHRGQGVAEALLNS
                     LEDYAAQSGYETLYLDSKKDLMPAIRFYRRHGYEFCERYNDNPQATIFMRKQISADIQ
                     QQK"
     gene            complement(3632..5020)
                     /locus_tag="VFA02_12855"
     CDS             complement(3632..5020)
                     /locus_tag="VFA02_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZU10785.1"
                     /translation="MSIKKNGSAEVEAQKETNSCTPLNSRRQFLRQIAGLSLGPPLVQ
                     WGPLFALGETRKPVVPSTQTPLSQDDEKFLDELEHQTFLFFWEQANPQTGLIKDRCNV
                     RVSDKYEAASIASTGFGLTAICIAEKRGFISYQDARLRVIATLEFLWKKLPTHRGFFF
                     HFANINTGDRIWDSEVSSIDTAILLCGILTCRQYFRDRDIIQLARAIFDRVDWSWLSE
                     DVSLLPMGWTPESGFLPYKWDYYSELMMLYLLGMGSSRHPLPPDAWNAWKRITFEYDG
                     LRYIGSFAPLFVHQYSQAWFDFRQKRDKYADYFQNSAIATEVHRRFCIELHKYFPDYG
                     DDLWGITASDSEKGYVIWGGPPAMGPIDGTVVPAAAGGSLPFLPDATMRVLKNIREHY
                     PQAWSRYGFVDAFNPLKNWYDTDIVGIDAGIILLMAENLRTGFVWETFMKNPEAQQGM
                     SKAGFKSYSPAT"
     gene            5145..6167
                     /locus_tag="VFA02_12860"
     CDS             5145..6167
                     /locus_tag="VFA02_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10786.1"
                     /translation="MKNRKIIWTTVAVAAVAAVTTLLLVRAHRLRLLSLRQVVIEGAV
                     VQNDPDVNRQLPIANVAVTATVDGKSVTTRSEASGYFKVVLQRSVLSNAPVAVSFRHP
                     SYVPFDLSLQSKRPGSLNKLYIVRMVPVQTKKMPAGPNTPAVTVISNLRVRYTINSRT
                     ETNVGSAVRTFQVVNQGNVPCNHQAPCSPDGRWKASSGSLTLDAGVDNSFENVRASCI
                     AGPCPFTRIDPGGFIHGGRKITVSALDWSDTATFLLEAEVYHSEITSNVRELYPVIFG
                     GTLNFTLPPTAEGVSLEAEVNSAPMVFPLGPDLNLSWATCTVETSAGQEKTQVYRCGL
                     KPGYKF"
     gene            complement(6234..8231)
                     /locus_tag="VFA02_12865"
     CDS             complement(6234..8231)
                     /locus_tag="VFA02_12865"
                     /inference="COORDINATES: protein motif:HMM:NF025681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP domain-containing protein"
                     /protein_id="HZU10787.1"
                     /translation="MLFTRRKFIALSGAAAGAGALNWSALAQTPNGGNLIWPRDRALP
                     VFPPALSFQAADLTSLSGDQQGLLVTLQGIVNRKLPRLYFYWGTDPTNQEWLVTSKIP
                     YKMVSDPMGLVDQYRQEISGAVIYDPNLPDTVNIATNLAGSKGGVIATADLAKSLNLP
                     ILDDLRGRFNTKLDAYTWALNNLWPRLSNRVVTAISPTSTVTVPNVQWTTLLQETRTI
                     HDGSNKALYTADLTPFLGGEAVYVRYQDANQNDGWGPSVSLVTIIADGNVIASFQPAS
                     AAEQPYLFDPDGSQTTSGWRFADHTSYFIYKFVAPAGTKQLTLQTEMWNQFLVTATNT
                     APSIQVANPNFRDYIVAVNAPVFWLDPEDTDESALFTEALKLMQPDAPYLGWFPQGHE
                     MTGVTLCGQNSSPVVAADFFYNGSALSGVYANVFSRKSCPPVPKIANKIYLTLTMVEG
                     DNIQYNQHRMRQIWDDPNRGQAPINWSVSVLLADIAPSMLHYFQQTQTENDLLVAGPS
                     GAGYTYPDVWPAATLPGFIQRSGQYMQRTGMNVLFAYNRNGSTDVPFSASIIDLYKQY
                     VPGLLGIVYNFESSSQISMMDGVPVATLLGMNDLSSSQSELANIASSWNGTSPLFIAG
                     GVESWNMTPTDVNTLVSSLGPQFVVLRGDVFFQLYKASQSS"
     gene            8439..8630
                     /locus_tag="VFA02_12870"
     CDS             8439..8630
                     /locus_tag="VFA02_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10788.1"
                     /translation="MIKFLLWCLLFFLCWPLALLALIAYPLVWLLLLPFRLIGIAVQG
                     ALNLVAAIIFFPGRLLRAI"
     gene            8716..9024
                     /locus_tag="VFA02_12875"
     CDS             8716..9024
                     /locus_tag="VFA02_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10789.1"
                     /translation="MCPTTEWNYAFDIHPENAEGSITTIAARASQSSFTKDGAPVKLE
                     VKARQLPSWMAVDNVANPAPQGPVRSAEPEKQITLVPYAAAKLRVTAFPELERSNSQS
                     "
     gene            complement(9061..11442)
                     /locus_tag="VFA02_12880"
     CDS             complement(9061..11442)
                     /locus_tag="VFA02_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZU10790.1"
                     /translation="MKPLKIVLTPTRNRRLNEMIGLMVLVVAGLLLLALFSYQPTDPS
                     FNTVVGNPAARPQNWTGLVGAYFSDLLLQMEGAAAFCLPLLMAALGWTWMRSLPAGSP
                     WAKFTGAALTMLFVPALFGLLPGHPRFLHGLPLEGLTGRLLADTLVHYLNYPGACIFA
                     IAMVAVAIYLSTTFSFNTAREWLSVRFAFLMAWRDRFANWRQARARRREEKKAAREAM
                     QREKEAAKELQMAARTEKAVPAPKKQPANPEAPAPAQAEFEPAPPQKSVWEQMPRATV
                     PDPEPEIAVKERADAEVHKVTVAPKSHNGFRLPPSTLLHRSEDTQIVREDELREEARV
                     LIEKCAEFDVHGQITQINPGPVVTTFEFKPEAGVKYSRVTGLADDLCLAMRAESILIE
                     RMAGKSTVGIQVPNRERETIWLRDVIEAENFAHSKSKLTLAMGKDINGRIVVADLATM
                     PHVLIAGSTGSGKSVAINAMIMSLLYKATPEQVRMILVDPKRVELGMYESIPHLFTPI
                     ITEPKLAANALRNAVREMERRLKLLASRSVRNIDQYNKLFENGTPSLFDNEEQEQKPL
                     PYIVIIIDELADLMMLDKANVEESITRLAQMARAVGIHLVLATQRPSVDVITGLIKAN
                     VPTRMSFRLATKVDSRTILDSNGAESLLGRGDMLFLPPGTSRLQRVHAPFVTEKEISA
                     VTEFWKKQGEAEYVQGFLESPKDEKGRELDMEDGGEDANDELYEDAVRLVLEFGKAST
                     SLLQRRLRIGYGRAAHLIDMMERDGIVGPAEGSKPREILKPPDWLSEVEQAMR"
     gene            complement(11642..12511)
                     /locus_tag="VFA02_12885"
     CDS             complement(11642..12511)
                     /locus_tag="VFA02_12885"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZU10791.1"
                     /translation="MNDYIISVLLGIVEGLTEFLPVSSTAHLRISEAFLHIDLGDPYW
                     KMYTIVIQLGAILALLLVFAGRIIEFIRTFPEGEEHDRTIWNHPLTLTLVAFVCTAIP
                     SFLLTKTIGKHLESLKVMAWSLLIGGIVMWVVDEWSTRSEPVTKHVEQMSLFQAIWIG
                     LCQVLSAVFPGTSRSMSTIAAGQIVRMDRPSALEFSFLLSIPTMIAATGYDLLKTLHP
                     KHAEGAEAIAPLVMTGHGWIVLAIGFVVSFFVALGVVEWFLQWVRRHGFVPFAVYRIL
                     VGILLLFFAARLA"
     gene            complement(12738..13112)
                     /locus_tag="VFA02_12890"
     CDS             complement(12738..13112)
                     /locus_tag="VFA02_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU10792.1"
                     /translation="MLNRGLQEHPANKIRHSRTSEKLLENSRHFLSSHPRAGYLPVTP
                     SFLSSAQPARPRDVNTRFGRKLRALRREHHWTQLQMADILGINRSYISEIERGRKSVS
                     LGMLEVIALGFEMSLAELLRDI"
BASE COUNT         3053 a         3626 c         3728 g         3008 t
ORIGIN      
        1 ctgcatccgc cacgaaagcc ggacgagttg cgaacaggac attttccatc tctccggaca
       61 cctgcgcttt ttgtccatgg gacgcgagac gtctttggct ccattgctga attggcaaac
      121 gctctcaggc tcataccggc cgcggttgaa ttgtttccgg tggaaagcgc gggacacgaa
      181 ttgcttaccc ggcacaatcg ttacgccttg cccttgaaaa tcgtggaaac gtttcgccgc
      241 ttcacaggca tggcatgtgc cggctcgacg ccaaactagc accctctgga atattctgcg
      301 aaaattgaag atgtatacag cgtgccggtc atcgccggga gctttccttg atggctccgg
      361 agaagccgaa ttttgctgtg cggaaaacga atcctatggc tacgaaactg gcaagtcccg
      421 cgcaggcgga cattttggaa gaagttgcgg agtggattga ggctttcgat caggtagtgg
      481 tggacgaagg cccggaacag gcaacagagc tgctgaatgc actgcggcag cgagcgcgtg
      541 aagcaggcat taaccctcct ggtgaaattc ggacccccta cctcaatacc attcccaaac
      601 atgatgaagt gccatatccg ggcgaccgcc agatggagcg ccgcgtcgag gccctgatcc
      661 gttggaacgc catggccatg gtccatgcac agaacaagaa ggatgccggc atcggtgggc
      721 acatctccac atattcatcg ctggccactt tgctcgaagt tggattcaac cacttcttcc
      781 atgccagcta cggcaaccag cccggcgact ttgtttattt ccagggacac gcttcccctg
      841 gcgtttacgc ccgcgcatat ctggaaggcc gtctgaacga cgaccatctg aagaacttcc
      901 gtcatgaact gcgcgacaaa cctggtctgt cctcttatcc acacccgtgg ctcatgccgg
      961 agttctggcg ctttcctacg gtctcgatgg gtatcgggcc gctgaacgct atctatcagg
     1021 cgcgctttat gcgctacctt gaaaatcgcg gtctgattga aaagaccccg cgcaaggtct
     1081 gggcctttgt tggcgacggc gaaaccgacg aagtggacac gcttggcgcc atcaccgttg
     1141 cctcgcgtga aaagctggac aacctcatct ttgtgatcaa ctgcaacctc cagcgtctcg
     1201 atggcccggt acgcggaaac aaacgcatta ttgatgagct ggaaggcgtc ttccgtggcg
     1261 ccggttggaa cgtcattaag gtcatctggg gttccgactg ggacaccctc ttcgagcgcg
     1321 accacaaggg cctgctgctc aagcgcatgg aagagtgcgt agacggtgaa taccaggcat
     1381 tcaaggccaa aggcggcgcg tatgtacgtc aggagttctt cggtaaatat cctgaactgc
     1441 tcgaactcgt cagcgacatg acagacgagc agctcgccag cctgcatcgc ggcggacacg
     1501 acccggtgaa aatctacaac gcctataagc gcgcggtaga acatcgcggc ggaccgaccg
     1561 tgattcttgc aaaaaccgta aaaggctacg gactgggcac aacagaagcc cgcaacgccg
     1621 cgcaccagga aaagaagctc acagatgaat cgttgaccac gtttgtgaag cgtttcgaca
     1681 ttcctgttcc ggaagaagcc gccaagcatg gcactccata caagccggca gacaatgctc
     1741 cggaactcca gtacatgcac gagcgccgca aggccctcgg cggatacatg ccgaagcgcg
     1801 aggtgcctgc tatccacttc aaggccccgg agcttgacta cttcggcgag tggacggcag
     1861 gctccaaagg ccgcgccgtc tccacaacga tgggctttgt cagcattctg cgccatttga
     1921 tgaaggaccc ggagttcggc aagctggtcg tgcccatcgt tccggatgaa ggccggactt
     1981 tcggccttga atcggccatc cggcaggtgg gtatctacac gcctgagggg cagaagtaca
     2041 agccgcacga tctggacatg ctgctctact accgcgaaga gaaagacggc cagatccttg
     2101 aagaaggcat tactgaagct ggttcgatgg cgtcgttcac agccgcaggt acagcctatg
     2161 cgaattatcg cgtgcctacg attccgttct acatgtatta ctcgatgttt ggcttccagc
     2221 gcgtaggcga tatgatctgg gccttcgccg attcgcgcgg caagggcttt ctgatggccg
     2281 gcaccgccgg tcgcacgacc atgcttggtg aaggtcttca gcaccaggac ggccacagcc
     2341 acgtgctcgc cagcacagtc ccgacttgcc tgagctatga ccctgccttt gtttacgagc
     2401 tggccgtcat tctgcaggac ggactccgcc gcatgtatca ggaaaacgag caggtcttct
     2461 actacatcac catgtacaac gaagactatg ccatgcctga gatgccacag acagaaggcg
     2521 tccgcgaggg catcgtgcgc ggcatttaca agtacaagtc cgccatcaag ggcaaggctg
     2581 atgcacagtt gttcggcagc ggccccatcc tgaacgaagc cctgcgcgcg cagcagattc
     2641 ttgctgaaaa atacggcgtg cagacagatg tctggagcgt gaccagctac aacgaacttc
     2701 gccgcgaagc gctgtctgta gagcgctgga accgcctgca tccggctgaa ccggagaaga
     2761 cgccatatgt cctgcaggca atcaagggcg caaaggggcc cattattgct gcaacagact
     2821 atatgaagtc agtgcctgat cagcttgcgc cgtggctgaa agaccgcctg gtcacgctgg
     2881 gcaccgacgg ctttggacgc agcgacaacc gcgaatacct gcgccgccac tttgagatca
     2941 acgccgaatc cattgttgcc gccactctct ccaagctggc ccgcgatggc aagttcgacg
     3001 caaaacgcgc ccaaaaggct tttgaagagc ttggcttgga taccgaaaag gtagacgcgg
     3061 caaaagcata accggccaca ttactgatgt aaacaaggcc cgctgcttca gcgggcctca
     3121 tttttgctgc tgtatatctg cagagatttg tttgcgcatg aatattgtgg cctgcggatt
     3181 gtcattgtag cgctcacaga actcgtatcc atgtctgcga taaaaacgga tggcaggcat
     3241 caggtctttc tttgagtcga ggtatagcgt ttcgtaaccg gattgcgctg cgtagtcttc
     3301 caggctgttc aacagtgctt ccgcaacacc ttgtccgcga tgttccggac gaacatacat
     3361 acgtttgatc tcgccggcct tcttcagtct gggcagtcgg cgcagagcga tgcagccaat
     3421 tgcctcatcg ctttctgaaa gggccagcca tatgcccgcg ccgttgccga aatactcgcg
     3481 ctcaaaaccc gcccggtctt cacgtgccag caccccgacg gcctcatagt attcacacac
     3541 gatgtcccag gcagcttcca tctcatcagc cgctgcacga tggattttga tctgggccaa
     3601 atgacgcatc cggtagtaat aactttatac gttatgtggc cggactgtaa ctcttgaacc
     3661 ctgcctttga cattccttgt tgtgcttcag gatttttcat gaaagtttcc caaacaaaac
     3721 cagtccgcag gttttctgcc atcaggagaa tgattccggc atctataccc acaatgtctg
     3781 tgtcatacca gttcttcagc ggattgaagg cgtccacgaa gccatagcgg ctccatgcct
     3841 ggggataatg ctccctgatg tttttcagga cgcgcatagt cgcatccggc aaaaatggaa
     3901 gcgagcctcc ggcggctgcc ggaacgacag ttccatcaat gggtcccatg gcaggtgggc
     3961 cgccccagat tacatagccc ttttcggaat cggaagctgt aattccccac agatcgtccc
     4021 cgtaatcggg gaaatacttg tgcaattcga tgcaaaagcg gcgatgtact tccgttgcaa
     4081 tggctgagtt ctggaagtag tcggcatatt tgtcgcgctt ctgccggaag tcgaaccagg
     4141 cttgtgagta ttggtggaca aacaatgggg cgaatgagcc gatgtagcga agaccatcat
     4201 actcaaaggt gatccgcttc catgcgttcc atgcatccgg cggcaaggga tgtctggaag
     4261 aacccattcc aagcagatag agcatcatca gctcactgta gtagtcccac ttgtagggaa
     4321 gaaaaccgga ttcaggcgtc catcccatcg gcaggagaga gacatcttct gacagccacg
     4381 accaatccac gcggtcgaaa atcgcgcgcg cgagctggat gatgtcgcgg tctcgaaaat
     4441 attggcggca ggtaagaata ccgcagagaa ggatggccgt gtcaatcgaa gagacttctg
     4501 agtcccagat cctgtcgccg gtattgatgt tggcaaagtg aaagaaaaat ccgcggtgcg
     4561 ttggcagttt cttccacaag aattccagtg tcgcaatcac gcgcaggcgc gcatcctggt
     4621 aggagatgaa tccgcgcttt tcagcaatac agatggctgt cagtccaaat ccggtagagg
     4681 caatgctggc cgcttcatat ttatcggaca cacggacatt acagcggtct tttatcagcc
     4741 ctgtttgcgg gttcgcctgc tcccagaaaa aaagaaacgt ctggtgctca agttcgtcca
     4801 ggaatttctc gtcatcctgc gaaagaggtg tttgcgtgga agggacaaca ggcttccggg
     4861 tttcgccaag tgcgaaaagc ggtccccatt gcacgagcgg tgggccaaga gaaaggcccg
     4921 cgatttgccg cagaaactgg cgccgggaat tgagaggtgt gcatgagttg gtttcttttt
     4981 gggcttctac ttcggcgctt ccatttttct ttatgctcaa tcgaaccatt tgcgctcgct
     5041 aaaaaagcgg ttttcaaata aatagatgca caacctggag caatgaatac tttgactggc
     5101 agcgtataga ttcagatgcg cgtccaatta tggtcggtgt gtgaatgaag aacaggaaga
     5161 tcatctggac aaccgttgcc gttgctgctg ttgcagcggt aaccaccttg ttactggtgc
     5221 gcgcgcaccg gcttagatta ctttctctgc gtcaggtagt gattgagggt gctgtggtcc
     5281 agaatgatcc tgatgtaaac agacaattgc ccatagccaa tgttgcagtg acggccacgg
     5341 ttgatggaaa gagcgtaaca acgcgttcag aagcgtccgg atatttcaaa gttgttttgc
     5401 aaagaagtgt gctctccaac gcgccggttg cagtcagctt tcggcatccg tcttatgtgc
     5461 cttttgattt gagtttgcaa agcaaaagac cgggaagcct gaataagcta tatattgtgc
     5521 gaatggtccc tgtgcaaacg aagaagatgc ctgccggacc gaatactccg gcggtaacag
     5581 tgatttccaa tcttcgagta agatacacga tcaattccag aactgaaacg aatgtaggca
     5641 gcgccgtcag gacctttcag gtggtcaatc agggaaatgt gccttgcaat catcaggcgc
     5701 catgttcgcc cgacggcaga tggaaagcgt cgtcaggatc tttaacgctt gatgcaggag
     5761 tagacaactc gtttgaaaat gtgagggctt cgtgcattgc agggccatgc ccatttacgc
     5821 ggatagatcc aggagggttt attcatggcg gacgtaagat taccgtttcc gcgctggact
     5881 ggtccgatac agctactttt ctgcttgagg ccgaggtgta tcacagcgag attacgtcaa
     5941 atgtccgtga attatatccg gtgattttcg gaggtacgct caatttcact ctacctccta
     6001 ctgccgaagg agtaagtctt gaagctgaag tcaacagcgc tccgatggtt tttccgttag
     6061 gccctgactt gaatctgagc tgggccacct gcacagtcga aaccagtgcc ggccaggaaa
     6121 agacgcaggt gtatcgctgt ggattaaagc cagggtataa gttttgattt gtaagcggag
     6181 cggcagggcg gtccagcatg attgcctgcc gggccgccct gcgcacgttg gacttatgag
     6241 ctttgcgagg ccttatacaa ctggaagaag acatcgccgc gcagcaccac gaactgtggc
     6301 ccgagcgagc ttaccagtgt attcacgtct gttggggtca tgttccacga ctcaactcca
     6361 cccgcaataa acaatggact tgtgccgttc cacgatgagg cgatatttgc gagttccgat
     6421 tgactgctcg acaggtcatt catgccgagc aaagttgcta ccggaacgcc atccatcatg
     6481 ctgatctggc ttgacgactc gaagttgtag acaatgccca gcagtcccgg cacgtattgt
     6541 ttgtacaggt caatgatgct tgcgctgaag ggaacgtctg tgcttccgtt gcggttatat
     6601 gcaaacagca cgttcatgcc tgtgcgttgc atatattggc cgctccgctg gatgaagccc
     6661 gggagcgtag ctgccggcca cacatccgga taggtgtaac ccgcgcctga cggccctgca
     6721 accagcaagt cgttctccgt ctgtgtttgc tggaaatagt gcaacatgct gggcgctatg
     6781 tctgcgagca gcacgctcac gctccagtta atgggcgctt gtccgcggtt tggatcgtcc
     6841 catatctggc gcatgcggtg ctggttgtac tggatgttgt cgccttccac catcgtaagt
     6901 gtgagataga ttttgttggc gatcttcgga acaggtgggc aggatttcct ggaaaacacg
     6961 ttggcataga ctccgctcaa agcagagcca ttgtagaaga agtctgctgc gacaacggga
     7021 gaactgttct gcccgcagag agtcacaccg gtcatctcat ggccctgcgg gaaccagccc
     7081 agataaggtg catcaggctg catcagcttc agcgcctcgg taaacagggc agattcatct
     7141 gtatcttccg gatcgagcca gaaaaccgga gcattcacgg caacgatgta gtcgcggaag
     7201 tttggatttg ccacctgaat gctcggagca gtattcgtgg ctgtaaccag gaactggttc
     7261 cacatttccg tttgtaatgt gagttgcttt gtgcccgcag gagcaacaaa tttgtaaatg
     7321 aaatatgagg tgtggtcggc aaaccgccag ccggaagtag tctgtgagcc atccgggtca
     7381 aacagatagg gctgttctgc tgcgctggct ggctggaacg aagcaatcac attaccatcg
     7441 gcaatgattg tgaccagcga aacagatggt ccccatccgt cattctggtt cgcatcctga
     7501 tagcgtacat aaacagcttc accgcccaga aatggagtaa ggtctgccgt atagagggcc
     7561 ttgttagagc cgtcatggat cgtgcgcgtt tcctgaagga gcgttgtcca ctgcacattg
     7621 ggtacggtca ccgtgctcgt tggactgatg gctgtgacga cacgattact taaccgtggc
     7681 cagagattgt tcaatgccca tgtgtaggcg tccagctttg tattgaagcg cccgcgcagg
     7741 tcatcgagta ttggaagatt caggcttttt gccagatcag cagtggcgat gacgccgcct
     7801 ttggatccag caagattggt cgcaatgttt acggtgtctg gaagattggg gtcgtagatc
     7861 accgcgccgg aaatttcctg gcgatactgg tctaccagac ccataggatc ggaaaccatc
     7921 ttgtatggta ttttgctggt tacaagccat tcctgattgg tggggtctgt tccccaatag
     7981 aaataaagcc ggggcagttt cctgttcaca atcccctgaa gcgtcaccag cagaccttgc
     8041 tggtcgccag agagtgaagt gaggtcggcg gcctgaaagg agagtgccgg agggaaaacg
     8101 ggaagcgcgc gatcacgcgg ccagatcagg ttgccgccgt tcggagtttg ggcaagagcg
     8161 gaccagttca aggcgccagc accagccgct gcgccactga gggcgataaa cttcctgcga
     8221 gtaaataaca tagggccttc ctttgcacag atgagagttg ggggagtctg caaagcaagc
     8281 tcagaccgca gatggtaacg attccatcgg caccgaacac aatagcgatt ccgggaaatt
     8341 gctgtcaaga gcagtgttac aaatccgtaa aatgcagcag acggcctgcg ggaactttca
     8401 gcccggccat acgtatcttt ttgaaagagg ccgtgtttat gatcaagttt cttctatggt
     8461 gtctgctgtt tttcctatgc tggccattgg cgctgctcgc attgattgct tacccattgg
     8521 tgtggctgct gctcctgccc ttcaggctga ttggcattgc agtccagggt gcgctcaacc
     8581 tggtggccgc gatcatattt ttcccgggac gccttttgcg ggccatataa cctgaattaa
     8641 ccgtgatttc ccacgccgcc tgtcgaggaa gactggctca agctgcgtac gcgcggtctg
     8701 actgccgatt ggcaggtgtg tcccaccacg gaatggaatt acgcgttcga cattcatccg
     8761 gaaaatgcag aaggatccat taccaccatc gcagcccgcg cctcacaatc ctcattcaca
     8821 aaagacggag caccggtgaa acttgaagtc aaagcgcgcc agttgccgtc ctggatggca
     8881 gtggacaatg ttgccaatcc tgcgccgcag ggtcctgtaa gaagcgctga acccgaaaaa
     8941 cagatcacgc ttgttccata cgctgccgcg aagctgcggg tcaccgcttt tcctgagctt
     9001 gagcgctcga acagccaaag ctgaacgtgc ctggagcacg ttcggagtaa cgatactggt
     9061 tcagcgcatg gcctgctcta cttcgctcag ccagtctggc ggcttcagga tttcgcgtgg
     9121 ctttgaacct tctgcggggc cgacaatgcc gtcgcgctcc atcatgtcta tcaggtgtgc
     9181 ggcccgtcca tacccaatac gaaggcggcg ctgtagcagg gaagtggagg ccttgccaaa
     9241 ctccagcaca agccggaccg catcttcata gagttcatca ttggcgtctt cgccaccatc
     9301 ttccatgtcc agttcgcggc ctttttcgtc cttcggtgat tcgaggaagc cctgcacata
     9361 ttctgcttcg ccctgctttt tccagaactc ggtgacggcg gagatttcct tctcggtgac
     9421 aaacggagcg tggacacgct gaaggcgcga tgttcccggc ggcagaaaga gcatgtcgcc
     9481 gcggcccagc aaagattcgg cgccgttgga atcgagaatc gtgcgtgagt ctacttttgt
     9541 ggccagacgg aaggacatgc gcgtgggcac attggccttg atgagtccgg tgatgacatc
     9601 cactgatgga cgctgtgttg ccagtaccag gtggatgccg acagcgcgcg ccatctgcgc
     9661 caggcgcgta atggattctt ccacattggc cttgtccagc atcatcaggt cggccagttc
     9721 gtcaatgatg atgacgatgt aaggaagcgg tttctgctcc tgctcttcat tgtcgaagag
     9781 gctcggcgtg ccgttttcaa agagcttgtt gtactggtca atattgcgca cgctgcgcga
     9841 ggccagcagc ttcaggcggc gctccatttc gcggacggca ttgcgcagcg cgtttgcggc
     9901 cagcttcggc tcggtaataa tcggcgtgaa gagatgcgga atgctctcat acatgccgag
     9961 ttcaacgcgc ttggggtcta cgaggatcat gcgcacctgc tccggcgtgg ccttgtagag
    10021 cagcgacatg atcatggcgt tgatggcgac tgacttcccg cttccggtag aacccgcaat
    10081 caggacgtgc ggcatggtgg caaggtcggc gactacgatg cggccattga tgtctttgcc
    10141 catcgccagc gtcagcttgc ttttggaatg tgcaaagttt tcagcctcga tcacgtcgcg
    10201 cagccagatg gtctcccgtt cgcggttcgg cacctgaatg ccaacagtgc tcttgcctgc
    10261 catgcgctcg atcagaatgc tttcggcgcg catcgcaagg caaagatcat cggcaaggcc
    10321 ggtaacacgg ctgtatttca cacccgcttc cggcttgaac tcaaaagttg tgaccaccgg
    10381 gcctggattg atttgcgtaa tctgaccgtg aacatcgaat tccgcgcact tttcaatgag
    10441 aacgcgcgct tcttcgcgca actcgtcttc gcggacaatc tgcgtgtcct cgctgcggtg
    10501 caagagcgtg ctcggcggca gccggaaccc gttatgcgac ttgggcgcta ccgtgacctt
    10561 gtgtacttca gcgtcggcgc gctcttttac ggcaatctcc ggttcaggat caggcacggt
    10621 ggcacgtggc atctgctccc acacgctctt ctgcggcggg gcgggttcaa actctgcctg
    10681 ggcaggtgca ggggcttcgg ggtttgcagg ttgttttttc ggagcaggca cggctttttc
    10741 cgtccgcgct gccatctgca gctcctttgc cgcctctttt tcgcgctgca tggcttcgcg
    10801 agcggccttc ttttcctcgc gacggcgggc acgcgcctga cgccagttcg caaaccggtc
    10861 acgccatgcc atcaggaagg caaagcggac ggaaagccac tcgcgcgccg tgttaaagct
    10921 gaaggtggtc gaaagataga tggcgacggc taccattgca atggcaaaga tgcaggcgcc
    10981 agggtaattc aggtagtgga cgagcgtgtc agcgagcaga cgcccagtca ggccctccag
    11041 cggcagcccg tgtagaaatc gcggatgccc gggcagcagt ccgaagagcg ccggcacaaa
    11101 aagcattgtg agggccgcgc ctgtaaactt tgcccacggg gaccctgccg gaagcgaccg
    11161 catccacgtc cagccaagag cggccatcag cagaggcagg cagaaggcgg ccgccccctc
    11221 catttgcagc agtaagtcgc tgaaataggc gcccaccagc cctgtccagt tctgcgggcg
    11281 cgcagccgga ttgccaacga ccgtattgaa tgatggatcg gttggctgat aggaaaaaag
    11341 cgcaagaagc agaaggccgg caacaaccag caccatcagc ccgatcatct cgttcaggcg
    11401 gcggttgcgc gtcggcgtaa ggacgatttt caggggcttc attgaagaca cgggttcccg
    11461 acggttctat cccccagagc agttttatta tcccagacca gaaagccggc ctggcaggaa
    11521 aagtttgatg ggggaatcca gtttggtagg gtgcgggatg cagcgctcag attcaaaatc
    11581 tggcgcctct gagtttcttc gctgaaatgt gctgcccatt ctctgcaccc tttgcaccct
    11641 gtcaggcgag ccttgccgcg aaaaacagca gcagaatccc cacaagaatc ctgtacaccg
    11701 caaacggtac aaatccgtgc ctgcgcaccc attgcaggaa ccattcgacg acacccaacg
    11761 caacaaagaa ggagacaaca aacccgatgg ccagtacaat ccagccatgc ccggtcatta
    11821 ccagaggagc gatggcctct gcgccttctg catgcttagg atggagggtc ttcagcaggt
    11881 cgtagccggt tgcggcaatc atcgttggaa ttgagagcag aaagctgaat tccaaagccg
    11941 agggccggtc cattctcaca atctgcccgg ccgcaatcgt agacatggag cgagaggtcc
    12001 cgggaaacac cgccgaaagc acctgacaca agccaatcca gatggcctga aataacgaca
    12061 tttgttcaac gtgctttgtg accggctcgc ttcgggtgct ccattcgtcc accacccaca
    12121 tcacaatgcc gccaatcagc agcgaccaag ccatgacttt cagactttcc agatgcttgc
    12181 caatggtttt ggtcaaaaga aaagagggaa tggccgtgca gacaaaggca accagtgtca
    12241 acgtcagagg atggttccat attgtgcggt catgctcctc accttcagga aaggtacgaa
    12301 tgaactcaat gatgcgtccg gcaaaaacca gcagcaatgc cagaatcgcg cccaactgga
    12361 tcacgatggt gtacatcttc caatatgggt cgccaagatc aatgtgcaga aacgcttcgc
    12421 tgatgcgtaa atgcgccgtc gagctgaccg gaaggaactc ggtcaaacct tccacaatgc
    12481 cgagcaggac agaaataatg tagtcgttca aaaatcctcc ctgttgatgt catccatttg
    12541 ccaccggtgg agttttgcat ggaaactcct gggaagcacg ccgtcttgaa gcactgcttc
    12601 tttaatagaa gcataaagaa ggcttcagta gaaggcgcag caggaatgaa aaacacacca
    12661 gacgtagctt ttgtatgatg agtgtcaggg cccggcgccg ctggttggat tgggatgatt
    12721 ttgaggcatc aaagatttta aatgtcccgc agcaattcgg ccagactcat ttcaaaccct
    12781 aatgcaatga cttccagcat tcccagagat acagattttc tgccgcgctc gatttcgctt
    12841 atgtagctgc gattgatacc gaggatgtcg gccatttgca gttgtgtcca atgatgttcg
    12901 cgccgcagtg ctcgaagttt tctgccaaaa cgcgtattta cgtcgcgagg ccgtgctggt
    12961 tgggcagagg acagaaatga gggtgttaca ggtaaataac ccgcacgcgg gtgggaacta
    13021 aggaaatgcc tgctgttttc aaggagcttt tcgcttgtgc gcgaatgcct gattttgttt
    13081 gccgggtgtt cctgaagacc tctgtttaac atttgcaaca atcctctcaa tgcttctcat
    13141 cgcccagccc ttggctttct tatcgtcagc ctttggcaaa tattcaccac ttccatctgc
    13201 tatccagacg gaagtggcgg tgcatccatt tcgttgttaa agggggaaga gaattgcaga
    13261 gagcagaatc aggtaactga tgtctgtctg gccgctggtt ccagtagcac ggcttcggga
    13321 acttcccggg ttctttgtgc tgccagggct tcctgaattc ttctgctcgc cagaatgcag
    13381 gcagtcagaa aacctatggc accaccaagg caaaa
//
LOCUS       DASRBV010000346        14293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_330812, whole genome shotgun sequence.
ACCESSION   DASRBV010000346 DASRBV010000000
VERSION     DASRBV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330812"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFA02_12895"
     CDS             complement(<1..729)
                     /locus_tag="VFA02_12895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU10793.1"
                     /translation="MAESILPGPHPRKPALLSSPARSWTLLLLVFLAVHFAALFSPSL
                     LDDADATHANAAQHMARSGDWVTLYVNGVRYLEKPPLPYWMVAVDYHIFGYNVFATHL
                     PETLGVLACAILAWFWTRRAYGDRAAFYAALGVLTSLGVFLWTRFFIPESILTFFIAL
                     ALYCFLTGLEDRRPARIYTMYAALAIAVLAKGLIAPVFFLAAVIPYLAITGEWRKWRQ
                     LRPVTGLLLFLAIAAPWHILAGIRN"
     gene            804..1781
                     /locus_tag="VFA02_12900"
     CDS             804..1781
                     /locus_tag="VFA02_12900"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZU10794.1"
                     /translation="MPAKPRPNTKSASGQHLRQLTATLRSNLEKCASDPDVDPVHDLR
                     TGTRRVQAMVEALLRERSDSALQEAADRWLAMLKKLRRAAAPVRDLDVHRKLLRKLVK
                     HGGGHTGAGQMVKTSTGVVTGPSPDVVEAPATALPVSAVEQQADHLDAWLHHARQHRA
                     TELKKKAAKWLTKFDEEAAAFEQAISTSAKTRRKTRGAAEVALEAFARLTHEMPHLSA
                     DNLHDFRKGAKKARYIAESAAGDAQAQAIAKALKKLQDDIGDWHDWLLLAEEAHDAPG
                     KHGHELIALISDERDRQLVQAMKTVERLRGKLLGEHLAITQSKKQKGHP"
     gene            complement(1808..3631)
                     /locus_tag="VFA02_12905"
     CDS             complement(1808..3631)
                     /locus_tag="VFA02_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10795.1"
                     /translation="MNTHLKALAGFALASALVAGHAQTASSAKSHRARKAAPAKPSVE
                     SQIESLRQEMNSQIEQLKQQLADRDAQLRQAQDAAAQAQAAATQAQQAAQAQQAAVTE
                     NTQAVNTLQSSVSDLKTNTQSIVTTVQEQQAQVKKQIENPDALHYKGITLSPQGSFIE
                     AATVYRNRALGADINTPFNSIPFAGSDNAKMSEFFGTGRQSRLALTAEGKLDDWILRG
                     YYEMDWLGTGVTSNNNQSNSYVLRQRQLWAQAESSNGWLFTAGQQWSLATQDKQGILN
                     KTELLPQTIDPQYNVGFVWARQYGARVVKSFFDKQFWAGISAENAQTLTPSCQATGTG
                     VSCPSNYLIGQAGNGGGLYNGGGAPGASSSAPLTTYSYNLAPDLLAKVVFEPKGFGHY
                     ELFGISRFFRNRVYPSSTSAAGAYNDATVGGGIGGSLYVPATKYFDIGLSGLWGDGVG
                     RYGSSTLPDLTVRPDGQLALLHGFSALGTAVFHASPRLDVYFHGGTDYAFRRYFAVPG
                     GTVGYGSPFISDAGCGVELLPGTSPQTGYQPTASKCGSPTKDIQEYTLGYWYDFYKGP
                     KGRFRQAIQYSYFERYGWNGTGGVPKGNDNVIETSFRYYMP"
     gene            3955..5754
                     /locus_tag="VFA02_12910"
     CDS             3955..5754
                     /locus_tag="VFA02_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU10796.1"
                     /translation="MESAWGDRIRALKNVPPVLKILWESGPAVVAWGLVLRVFVALMP
                     WAIARVAAWIMNGVEHALRHQGVPQYFWWVVGLEVSLAVLNGLLTRTIDFFDALLADR
                     YTHHVSIEVMRKAAQLDLTTYEDPVYYDRLERARVQATDRLVLIQQLGRLFQQIITTL
                     IFTGTLLYYSPWLLLLLALGVLPSFLGETHYAFLGYAKNFRQTPIKRLMDYLRQVGGS
                     KEAAKELKLFNLNEYLTNRFAGLAEQIYKENIALSRKKLIGGGLLGILSTLGYYGSYA
                     YVIWRAVHGYYSIGIFYFITNAIVQASSNLQQVFSTASGIADQALFLTDLIGFFEMKP
                     TVQSKPNALPAPRPIQRGFEFRNVSFAYPGTERKVLKNFNFTLRPGERIALIGENGQG
                     KTTVVKLITRLYDPTEGQILLDGIDLREYSLEDLHKEIGVIFQDFMRYEMTVRENISI
                     GRVEIPHTEDEIQSAAQKSLASGVVAKLQNGYDQILGRRFEGGVDLSGGEWQKIALAR
                     AYLRDAQLLILDEPTAALDARSELEVFERFAELTEGKMALLISHRFSTVRMADRIVVL
                     EGGKLVEEGNHQQLMALGGRYAEMFEMQAASYR"
     gene            5769..10238
                     /locus_tag="VFA02_12915"
     CDS             5769..10238
                     /locus_tag="VFA02_12915"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZU10797.1"
                     /translation="MTEQTTSSIETTPLPPGLREAAHRAAQSWHVTHRPQGKGGFTLR
                     MEAARDSLEQLFAHLDTYEPGPNPGPEPLLEIRENPRLFRSVVIEAFSIRRKIERLPR
                     VVTKEGEEPRVATLAAAYLDAAHSSWADEALLAFLDEVQKVDPLHLQELWALPVMLKL
                     LLLEWILSQASARLHSPNSSAAGTADTLRTRIQTLREISFAGWPGLIEQAIVFEPVLQ
                     QDPAQAYGHMDFESREQYRKRVAEIALYSEVTELEVARCALEMAKEAQQQQISDPRLY
                     LRRAHVGYYLIDKGFRELARRIHYNPRMIDKLRMAIQRNADDFYIGSIEVLTVILIAA
                     ILLPLVPTYPIFGSLAFAFLLMLLPVTQGAVDLVNNTVTSLFKAHALPKLDFSSGIPP
                     EFTTLVAVPTLLLNEKQVRELVQDLEVRFLANQDPNLHFALLTDLPDSITRPRENDTD
                     PLVDLAVSLIHELNARYKSPKHGSFLLLHRHRLFNAKQGVWMGWERKRGKLLDLNKFL
                     LGAFDAFPVKAGNLEVLKHVHYIITLDSDTQLPRGAAHSMIGAMAHPLNRAIIDPQRR
                     IVTDGYGILQPRVGVSVHSASRSRLAATYSGQTGFDIYTRAISDVYQDLYGEGIFTGK
                     GIYEVATLHAVLDRRFPRNSLLSHDLIEGAYARAGLATDIEVIDDYPSHYSAYTRRKH
                     RWVRGDWQIVQWLFSRVPDESGHYVRNPISTISRWKIFDNLRRSLVEPLTMILLVAGW
                     LGLPGGPLYWTLVTLLLLFLPTFVQLLFSAGRALFSEQQGAMREAFTGFLQGAFIALL
                     NLIFLPHQTMLTIDAIVRSLVRRFITGQRLLEWETAAEAESTAQRTTPVDRYLALIPL
                     FSIILAVIVLAVSPRALLVAAPILVLWGFSGGITYWLNQPPLDEARRLPKNDEAFLLE
                     HALRIWRYFYQFGGESHNYLIPDNVEEDGLFEAARVSPTNLGLLLNARQAAVEFGFLT
                     VPEFATLTHASFATIAKLEKFRGHLLNWYDTRTLKAFQPAFVSSVDNGNLVASLYTLR
                     SGAQSLLKEPLISQKLFHGLQVFYALLQAEVPGLVKKIPAPPPRTAKPDEWIAWTMQS
                     DAAEWGGLAVPESEDPWWAAEMQVRIRAAQSLVEDYMPWLLPEFLPLRSVPQLGLRDE
                     TIFPRLEEAAAFAEQLRGRLSRAWATGDEDGNLFLGEKLRSLLPAAEERLRNLVQSVQ
                     AVADDTSRLIEEMDFSFLLQKGRILLSIGYDVSADKLHAACYDLLASEARIASFIAIA
                     KGDVPQQVWFKMARMHTMAYGRMLLLSWTGTMFEYLMPSLWMRSYPDTLVSRTLYAAV
                     EVQQAYGREHRIPWGISESGYADKTEDGHYHYQAFGIPQLALKYDASAGPVVSPYSTF
                     LALGVDPTESIRNLRRMASYGWVGAFGFYEAADFMRSKKDPQLAREWMAHHQGMSLLA
                     LLNLLHDDKTKQWFHANPELQATELLLHEKPINEAYLKFEYKQFAPRKLKKAG"
     gene            10306..11529
                     /locus_tag="VFA02_12920"
     CDS             10306..11529
                     /locus_tag="VFA02_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10798.1"
                     /translation="MADTETIPPEQPVEMHPHHRSLLPFLGVACGVSVSSIYYNQPLL
                     LEMSRSLRAAHGSMGSVAVATQVGYSLGILAFVPLGDVVERRSLMVRLFAGVALSALL
                     CAFAPTLAVLIAASVAIGLTAAVTHVIVPIAPELAGDEERGRAVGTVMTGLLLGVLLA
                     RTVSGWIASFLGWRAVFIFAAAASFTFVFLMVRRLPKLPPHTPLPYGDALRSLWTLTR
                     TQPLLRESAVLGGLAFGAFSAFWTTLVFLLGSGHYRLGAGVAGSFGILGATGALIAPI
                     AGRVSDKHGSRAVVTLGLGLLATGFCILWIFGYHMAGLIFGVVVLDLGMQASQIANQT
                     RIFGLEPGARGRINTIYMTVYFLGGSLGSLFSTIAWAHWGWSGVCALGLIMLALAALI
                     HATGVRAGEPVSVPS"
     gene            complement(11575..12636)
                     /locus_tag="VFA02_12925"
     CDS             complement(11575..12636)
                     /locus_tag="VFA02_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU10799.1"
                     /translation="MLRRSSIFVFMAAMLFLVRSYAQEPGSPDAPPVSNAPDTTQQEN
                     TATPTFKANVNLVSIYFTVRNKQGGLVPDLTKDDCAIFEDKQPQKIKNFAPETDLPLT
                     LGILLDTSGSQQNVLPMEQQTGAAFLKRILRPKDEAFLVSFDVQVSLLQDYTSNPRLL
                     EKAMDQAVINTGGGGGAIGVPGMGQGPVPVHGDPKGTLLYDAVYQASDKLRSETGRKA
                     LILLTDGEDQGSMHKIADAIEEAQKSNTIIYALLIADRGFYYGYGMGYTGASALKKMA
                     EETGGRMIDVGNNGKKMEAAFRQIEDELRTQYLASYTPTNNKMDGSFRKLDISCKGDG
                     LKVQARKGYYAIPEGDEGQ"
     gene            12908..14221
                     /locus_tag="VFA02_12930"
     CDS             12908..14221
                     /locus_tag="VFA02_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU10800.1"
                     /translation="MQGALAVLLAFLMLGVPQQQQQQSIPDAPAPQPASSSDLSNLKG
                     QIAPGKGTSSEALQQSAPAAQQPAPNQQQPSASSSDTQSAPQPQEDNGQQQQAPYIPK
                     PGEQQAFLIRVPVTYVDVPVTVRDKNHHLVAGLTWRQFKIYEDGVRQKIAFFSVDPRP
                     LSVAFVIDQTLPSDVMQKVNQALSAVTGAFTPADSVAVFAYNSSPRIITEFTGAQGAR
                     LPAALQSAKASGRDMGVISPGGPLDSGPTINGKLVDPNVAPQRGNMGFITIPKEVHPL
                     NDAILEAAKALAQQPKGNLRVLYVISDGKESGSQASYKEVVRYLLTNNIVLYGTLVGD
                     SAMWGVGYLDKWHIPLVPTMRDNILPKYALATGGALESQYSENGIQKSFATIASSART
                     QYFLGYYTHSGIMSDKFHKITVVVEAPGLDITAKDGYYPSASMRQ"
BASE COUNT         2983 a         4219 c         4063 g         3028 t
ORIGIN      
        1 attgcggatc cccgccagaa tgtgccatgg cgcggcgatc gccaggaaca acagcagacc
       61 cgtcaccggg cgaagctgcc gccacttgcg ccactcgccg gtaatcgcaa gataagggat
      121 gaccgctgca agaaagaaca cgggcgcaat cagccctttt gccagcacag cgatggcgag
      181 cgctgcatac attgtgtaga tacgggcagg cctgcggtct tcgagtccgg tgagaaaaca
      241 gtacagtgcc aaagcaatga aaaaggtcag aatggattcc ggaataaaga agcgcgtcca
      301 gagaaagacg ccgaggctgg tcagtactcc gagagcggca taaaaagcgg cgcggtcacc
      361 gtatgcgcga cgcgtccaga accatgcgag aatggcgcag gccagcactc ccagcgtttc
      421 cggcagatgg gtggcaaata cgttgtagcc aaagatgtgg tagtccaccg cgaccatcca
      481 ataaggcagt ggaggctttt ccagatagcg gacaccattc acgtacagag ttacccagtc
      541 gccgctcctg gccatatgct gagcagcatt ggcgtgcgtg gcgtcggcgt cgtccagaag
      601 agagggcgaa aacagcgcgg cgaaatgcac tgcgagaaaa accagcagca gcaatgtcca
      661 gcttctggct ggagaagaaa gaagcgcagg tttgcgagga tggggcccag gcaagattga
      721 ttcagccatg atttttccga atctttagaa tatcagcggt gttggtgtaa tttaccgcag
      781 agggcgcagg agagaagcga atcgtgcctg caaaaccacg accgaataca aagagtgctt
      841 caggccagca tctgcggcaa ctgactgcca cgctgcgcag caacctggaa aagtgcgctt
      901 ctgacccgga cgtagaccca gtgcacgacc tgcgcaccgg aacgcgtcgc gtacaggcga
      961 tggtggaagc actgctgcgc gagcgcagcg attctgcgct tcaggaagcg gcagaccgat
     1021 ggctcgcgat gctcaagaag ctgcgccgcg cggctgcacc tgtgcgcgat ctcgacgtgc
     1081 accgtaagct tctgcgcaag ctggtgaagc acggcggcgg ccacaccggg gccgggcaga
     1141 tggtcaagac cagcaccggc gtggtcaccg ggccgtctcc ggatgtggtt gaagctcccg
     1201 cgactgcatt gccggtcagc gcagtcgagc agcaggcaga ccacctcgac gcatggctgc
     1261 atcatgcgcg gcagcaccgg gccaccgagc ttaaaaagaa agcagcaaag tggctgacaa
     1321 aattcgatga agaggccgcc gcatttgagc aggccatcag cacctcagca aaaacacggc
     1381 gcaaaacccg aggcgcggca gaggttgcgt tggaggcatt cgcccgcctg acacacgaga
     1441 tgccgcatct cagtgcagac aatcttcatg atttccgcaa aggcgcaaag aaggcccgct
     1501 atatcgccga atcagcagcg ggggatgccc aggcccaagc catcgcaaag gcactcaaaa
     1561 aattgcagga cgacattggc gactggcatg actggctgtt gctggcagaa gaggcccacg
     1621 acgccccagg taaacacggc cacgaattga tagccttgat cagcgatgaa cgcgaccgtc
     1681 agcttgttca ggcgatgaag accgttgaac ggctccgcgg aaaattattg ggcgaacatc
     1741 tggccatcac gcagagcaaa aaacaaaaag ggcacccgta gcgggtgccc tgtaagcggg
     1801 aggcaaatta cggcatatag tagcggaacg aagtctcgat gacgttgtcg ttgcccttcg
     1861 ggacaccgcc tgtaccgttc catccatagc gttcaaagta gctgtactgg atggcctggc
     1921 ggaagcgtcc tttcggtccc ttgtagaagt cgtaccagta gcccagggta tattcctgta
     1981 tgtccttggt ggggctgccg cacttggatg ctgtaggctg atacccggtc tgtggcgaag
     2041 tcccgggaag cagttccact ccacaacccg cgtcgctgat gaacggggag ccatatccca
     2101 cggtcccacc cggtacagca aagtagcgcc ggaaggcata atccgtaccg ccgtggaagt
     2161 aaacatcgag gcgtggcgaa gcatggaaga ccgcggtgcc cagagctgag aagccatgca
     2221 gcagggcaag ctgcccatcc ggacggaccg tcaggtcggg cagcgtagag gagccgtaac
     2281 ggcccacccc atcgccccac aatccgctga ggccaatatc aaagtacttc gttgccggga
     2341 catagaggct gccgccgatg ccaccgccga cggtcgcatc gttgtaagct ccggctgcgg
     2401 aagtcgagga aggatagacg cggttgcgga agaagcgcga aatgccgaac agctcgtagt
     2461 gtccgaaccc ttttggttcg aagacgactt tggccaggag atctggagca aggttgtaag
     2521 aatatgtcgt gagaggtgca gaggatgagg caccaggcgc cccgcccccg ttataaagac
     2581 ctccgccatt ccccgcctga ccgatcagat aattcgacgg gcaggagacg ccggtccctg
     2641 ttgcctggca gcttggcgta agcgtctgcg cattttctgc cgaaataccg gcccagaact
     2701 gcttgtcaaa gaagctcttt accacgcgag cgccatactg acgtgcccac acgaagccga
     2761 cgttatactg cgggtcaatg gtctggggca gcagctcggt cttgttcaga atgccctgct
     2821 tgtcctgcgt ggccagcgac cactgctgtc ctgcggtgaa gagccagcca ttggaagact
     2881 cggcctgtgc ccacaactgc cgctgccgca atacgtaact gttgctctga ttgttgttgg
     2941 aggtcacgcc cgtgcccagc cagtccatct catagtagcc gcgcaggatc cagtcatcga
     3001 gcttgccttc agccgtgagc gccagacgtg actgacggcc ggtgccaaaa aattcgctca
     3061 ttttggcatt gtctgaacct gcaaacggaa tggaattgaa gggcgtgttg atgtctgcac
     3121 ccagtgcgcg gttacggtag accgtcgcgg cttcaataaa gctgccttgc ggcgaaagcg
     3181 taattccctt gtagtgcagc gcatcagggt tctcgatctg tttcttcacc tgcgcctgct
     3241 gctcctgcac cgtggtcaca atggactggg tgttggtttt caggtcagaa acagagcttt
     3301 gcagagtatt gaccgcctgt gtgttctctg tcacggcggc ctgctgggcc tgggcggcct
     3361 gttgcgcctg ggtggcggcg gcctgtgcct gcgctgccgc atcctgcgcc tgcctgagtt
     3421 gagcatcgcg gtcggcgagc tgctgtttca actgttcaat ttgcgaattc atctcctgcc
     3481 gcaacgattc aatctgcgac tccaccgagg gctttgccgg agcagctttt ctggcccggt
     3541 ggcttttcgc agaagaagca gtctgggcat gacccgcaac cagcgccgat gcaagcgcaa
     3601 agcctgcaag cgccttcaaa tgtgtgttca tttggcctct cttcacaact tttggcactc
     3661 cagcgcacac gtgtacgtct gccttctggc aaacaaggat tgctggagtt cgtttccagc
     3721 ttcccaagtc agcgttaagg aaaagagaat agagtgtgaa tagactgcaa aaacgaatgt
     3781 caggcaggtc cattggagga tggcgggagt gcctgtgaag gtcctgttaa acaacgttcc
     3841 agcagcagtt tgcatcgaaa aacatgattc cactcaggat gtgaccgttc gccgcgcgcc
     3901 attgaagaag aacagggtct aattttgtcc atggagcaga aagaaagcga aaaaatggaa
     3961 agcgcctggg gcgaccgcat ccgcgcgctt aaaaatgtcc cccctgtcct gaagatcctt
     4021 tgggaatcag gcccggctgt tgttgcctgg ggactcgtcc tgcgcgtatt tgttgcgctc
     4081 atgccctggg ccattgcacg tgtggccgcc tggatcatga acggcgtcga gcatgcgctg
     4141 cgtcaccagg gtgttccgca gtatttctgg tgggttgtcg gccttgaggt ctcgctcgct
     4201 gttttaaacg ggcttctcac ccgcaccatt gatttctttg acgccctgct tgccgaccgc
     4261 tacacacacc acgtcagcat cgaggtaatg cgcaaggccg cgcagctcga cctgactact
     4321 tacgaagacc cggtttacta tgaccgcctg gagcgggcac gcgtgcaggc caccgacaga
     4381 ctggtgctca tccagcagct cggccgtctt ttccagcaga tcatcaccac gctcatcttt
     4441 accgggaccc ttctgtacta ttcgccgtgg ctgcttctgc tgctggcgct cggtgtgctg
     4501 ccgtcgtttc tgggtgaaac acactacgcc tttcttggat atgcgaagaa tttccgtcag
     4561 acccccatca aacgcctgat ggattatctg cgccaggtag gcggcagcaa agaagcggcc
     4621 aaagagctga agctcttcaa tctgaatgag tatctgacga accgctttgc cgggctcgcg
     4681 gagcaaattt acaaggaaaa cattgccctc tcgcgcaaga agctcatcgg cggcggactg
     4741 cttggcatac tcagcacgct tgggtattac ggctcttacg cctacgtcat ctggcgcgcc
     4801 gtgcatggat actacagcat cggcatcttc tacttcatta ccaatgccat tgtgcaggcc
     4861 agctccaacc ttcagcaggt tttctcaacg gcgtccggca tcgccgacca ggcactcttt
     4921 ttaacagacc tgatcggctt ctttgagatg aagccgaccg tgcaatcaaa gcccaatgcc
     4981 ttgcctgccc cgcggcccat ccagagaggg tttgaattcc gcaacgtatc gttcgcgtat
     5041 ccgggaacgg agcgcaaggt cctcaaaaac tttaacttca cgctgcgccc gggtgagcgc
     5101 attgccctaa ttggggaaaa tggccagggc aagaccacag tcgtcaaact gatcacgcgc
     5161 ctctacgatc cgacggaagg acagattctg ctcgacggca ttgacctgcg tgaatattcg
     5221 ctcgaagacc tgcacaaaga aatcggcgtg atctttcagg acttcatgcg ctatgaaatg
     5281 acggtgcggg agaatatttc cattgggcgg gtggaaatac cgcataccga agacgagatt
     5341 cagagtgctg cgcagaagag tcttgcatca ggcgtggttg ccaaattgca aaatgggtat
     5401 gaccagatcc ttggacgccg ctttgaaggg ggtgttgatc tttccggcgg tgaatggcag
     5461 aaaattgcgc tggcacgtgc ctatctgcgc gacgcgcagc ttttgattct ggacgagccg
     5521 accgctgcgc tcgacgcccg cagcgaactg gaagtttttg agcggtttgc ggaactgacg
     5581 gaaggcaaga tggccctgct gatctcgcac cgcttttcga ccgtccgcat ggccgaccgc
     5641 atcgtagttt tagaaggcgg caagcttgtt gaagaaggca atcatcagca actgatggcg
     5701 ctgggcgggc gctacgcaga gatgtttgaa atgcaggccg caagttaccg ataaacattg
     5761 caggacgaat gacagagcag accacatcaa gcattgaaac aacgccattg cctcccggat
     5821 tgcgtgaggc cgcgcaccgg gcagcccaaa gctggcatgt aacacatcgc ccgcagggga
     5881 aaggcggctt tacgctgcgc atggaagcgg cgcgcgattc gctggagcag ttgttcgcgc
     5941 acctggacac ctatgagccg ggccccaatc ccgggccgga gccgctgctg gaaatccgcg
     6001 agaacccgcg cctgtttcgc tctgtcgtga tcgaagcctt ttcgattcgc cgcaaaattg
     6061 agcggctgcc gcgtgtggtc acgaaagaag gcgaagagcc gcgcgtggcc actctggctg
     6121 ccgcttatct tgatgcagcg cactcttcct gggccgacga agcgctgctt gcatttcttg
     6181 acgaagtgca gaaggttgat ccgctgcacc tgcaggaact gtgggcgctt cctgtgatgc
     6241 tgaagctgtt gttgctcgaa tggattcttt cgcaggccag cgcccgcctc cattccccga
     6301 actcttctgc cgcaggtaca gctgacacgc tgcgcacccg tatccagacg ctgcgggaaa
     6361 tcagctttgc cggatggcct ggattgattg agcaggccat tgtctttgaa cccgtgcttc
     6421 agcaggaccc ggcgcaggcc tatggccata tggacttcga gagccgcgag caataccgca
     6481 agcgcgtagc cgagatcgcg ctttactccg aagttacaga actcgaggtg gcccgatgcg
     6541 cgctggaaat ggcgaaggag gcgcaacagc agcagatttc cgatccgcgt ctttatctgc
     6601 gccgcgcgca cgttggctac tacctcattg acaaaggctt ccgcgagctt gccaggcgca
     6661 ttcactacaa cccgcgcatg attgataagc tgcgcatggc catccagcgc aatgcagacg
     6721 acttctacat cggctccatt gaagtcctga cagtcattct gattgccgcc attctgctgc
     6781 cactggtgcc gacatatcct atttttggca gccttgcgtt tgcattccta ctgatgctgc
     6841 ttccggtcac gcagggcgca gtggacctgg tcaacaacac tgtcacctcg ctctttaagg
     6901 cgcacgccct tcccaagctc gatttttcaa gcggcattcc acccgagttc acaacgctcg
     6961 tcgctgttcc aacattgttg ttgaacgaaa agcaggtgcg cgagctggta caggaccttg
     7021 aagtgcgctt cctggccaat caggacccca acctgcactt cgctctgctc acggatctgc
     7081 ctgactccat cacgcgcccg cgcgaaaacg atacagaccc gctggtggac cttgctgttt
     7141 cactcatcca tgagctgaat gcgcgctaca aatcgccaaa gcatggatca tttctcctgc
     7201 tgcaccgcca ccgccttttt aatgcgaagc agggtgtatg gatgggctgg gaacgaaagc
     7261 gcggcaagtt gctcgacctg aataaattcc tgctcggggc atttgacgct ttcccggtca
     7321 aggcgggaaa tctcgaggtg ctgaagcacg ttcactacat catcacgctc gattcagata
     7381 cgcagctccc gcgcggagca gcgcactcca tgattggcgc catggcgcac ccgctgaacc
     7441 gggccatcat tgatccacaa cggcgcatcg tcaccgatgg atacggaatc ctgcaacctc
     7501 gtgtgggtgt cagcgtgcat tcggcctcgc gttcgcgcct ggcggccacg tattccggcc
     7561 agacggggtt cgacatctac acacgcgcca tctctgacgt ctaccaagac ctctacggcg
     7621 agggcatctt taccggaaaa ggcatctacg aagtcgcaac gcttcatgcg gtgcttgacc
     7681 gccgtttccc acgcaactct ctccttagcc atgacctgat tgaaggggcc tacgcccggg
     7741 ctggtcttgc cactgatatc gaagtcatag atgactatcc ttcgcactac tccgcctaca
     7801 cgcgccgcaa acaccgctgg gtgcgcggcg actggcagat tgtgcagtgg cttttttccc
     7861 gcgtgccgga tgagtccggc cattatgtgc ggaatccgat ttcgactatt tcgcgctgga
     7921 agatcttcga caatctgcgc cgctctctgg tcgagccgct cactatgatt ctccttgtcg
     7981 caggctggct cgggctgccg ggtggtccgc tttactggac gctggtcaca ctgctgctgc
     8041 ttttcctgcc tacttttgtg cagctgctct tcagcgccgg acgggccctc ttcagcgaac
     8101 agcagggcgc catgcgagag gcattcactg ggttcctgca aggcgcgttc attgcactgc
     8161 tcaacctgat tttcctgccg caccagacca tgctgaccat tgacgccatt gtccgctcgc
     8221 tggtgcgccg gtttatcacc ggacagcggc tcctggagtg ggaaacagca gcagaggccg
     8281 agagcacagc gcagcgtacc acgcctgtgg accgctacct tgctctcatt ccgctatttt
     8341 cgatcattct ggcggtcatt gttctagctg tctctccacg cgcgcttctg gtggctgctc
     8401 ccatccttgt gctgtggggc ttttccggag gcattacata ctggctcaac cagcctccac
     8461 tcgatgaagc gcggcgtctg ccgaagaacg atgaagcatt tctgctggaa catgcgctgc
     8521 gcatctggcg ctacttctat cagttcggcg gagaaagcca caattatctg attccggaca
     8581 acgtggaaga agacggccta tttgaggctg cccgggtttc gcctaccaat ctcggcctgc
     8641 tgttgaacgc gcgccaggca gccgtggagt tcggctttct caccgttccg gaatttgcca
     8701 cacttacgca cgcgagcttc gccactattg caaagctgga gaaatttcgc ggccatctcc
     8761 ttaactggta tgacacgcgc acgctcaagg catttcagcc cgcattcgtc tcttcggtgg
     8821 acaacgggaa cctggtggca tcgctctaca cgcttcgcag cggggcacag tcgctgctga
     8881 aagagccgct catctcgcaa aagctctttc atggactcca ggtcttttat gcgctgcttc
     8941 aggcagaagt ccccggactc gtaaagaaga ttcccgctcc gccgcccagg acggcaaagc
     9001 cggatgaatg gattgcgtgg accatgcaat cggacgcggc agagtggggc ggccttgctg
     9061 ttcctgaaag tgaagacccg tggtgggccg ccgagatgca ggtgcgcatc cgagcggcgc
     9121 agtctctggt ggaagattac atgccctggc ttctgccgga gtttttgccg ctgcgttctg
     9181 ttccgcagct tggtctgcgc gatgagacca tctttccacg tctggaagaa gccgccgcct
     9241 tcgccgaaca actcagggga cggctctccc gcgcatgggc aaccggagac gaagatggca
     9301 acctcttcct gggagaaaag ctgcgttcgc ttctgcctgc cgcggaagag cggctgcgca
     9361 atcttgtgca aagtgttcag gccgtcgccg atgacacgtc ccgtttgatt gaagaaatgg
     9421 acttctcctt ccttctgcag aagggacgca tcctgctttc gattggctac gatgtttcgg
     9481 cggacaagct gcatgccgcc tgctatgact tgcttgcgtc ggaagcgcgc atcgcttcct
     9541 ttatcgccat tgcaaagggc gatgtgccgc aacaggtatg gttcaagatg gcgcgcatgc
     9601 acaccatggc ctacggacgc atgttgctgc tctcctggac cggcaccatg tttgaatacc
     9661 tcatgccgtc gctctggatg cgcagctatc cggacacgct ggtttcgcgg accctgtacg
     9721 ccgcggtcga ggtacagcag gcctatggac gcgaacaccg cataccgtgg ggcatttccg
     9781 aatcaggcta tgccgacaag acagaagacg ggcactacca ctaccaggca tttggcattc
     9841 cgcaactggc gctcaagtac gatgcgagcg cgggtccagt cgtctccccc tactccacct
     9901 tccttgcgct cggagtagac cccacggagt ccatccgcaa cctgcgccgc atggccagct
     9961 atggatgggt gggcgcattc ggcttttatg aagcggcaga ctttatgcgc tcgaaaaagg
    10021 acccgcaact ggcccgtgaa tggatggcgc accaccaggg catgtcactt ctggcgctcc
    10081 tgaatctgct gcacgacgac aaaacaaagc aatggttcca cgcgaaccct gaacttcagg
    10141 caacagaact tctgctgcac gaaaaaccaa tcaacgaggc atacctcaag ttcgagtaca
    10201 aacagtttgc tccacgcaaa ctgaagaaag ccggctaagt tgaaaggatt gagcgaagga
    10261 ttgagcgagg aatggggcca tctctctcat cgaataaact aaaacttggc agacacagag
    10321 accattcctc ccgaacagcc cgtagagatg catccacatc accgcagcct tttgccattt
    10381 cttggcgtgg cctgcggcgt aagtgtttcc agtatctatt acaaccagcc cttgttattg
    10441 gaaatgtccc gcagcctgcg cgcggcgcac ggcagcatgg gctctgtggc cgttgccacg
    10501 caggtcggct actcgctcgg aatcctcgcg tttgttccac tgggagacgt ggtggaaagg
    10561 cgcagcctca tggtccgcct ctttgccggg gtcgcgcttt ctgctttgct atgtgcgttc
    10621 gcgccaacat tagcagtcct catcgccgcc agtgtcgcca tcgggctcac cgcagcggtc
    10681 acgcacgtca tcgtacccat cgcacctgaa ctggccggcg atgaagaacg cggccgggct
    10741 gtcggcacag tcatgaccgg gctgctgctg ggtgtattgc tggcacgcac tgtttccggc
    10801 tggatcgctt catttttggg gtggcgagcg gtcttcattt ttgctgccgc cgccagtttt
    10861 acctttgtgt tcctgatggt acgcaggcta cccaagctgc ctccgcacac acctctgcct
    10921 tatggcgatg cgctccgttc tctttggaca ctcacccgca cgcaaccgct cctgcgcgaa
    10981 tcggcggtgc tcggcggact ggccttcggt gccttcagcg ctttctggac gacgctggtc
    11041 tttctgctcg gctccggcca ttaccgtctg ggagccggcg tcgccggcag cttcggcatt
    11101 ctcggcgcaa ccggagcgct gatcgcgcct atcgccgggc gcgtctcaga caagcacggc
    11161 tcgcgcgccg tcgtgactct ggggctgggc ctgctggcga ccggtttctg cattctctgg
    11221 atcttcggtt accacatggc cggattaatt ttcggtgtcg ttgtgctcga tcttggaatg
    11281 caggccagcc agattgccaa tcagacgcgc atctttggac tggaaccggg cgcacgcggc
    11341 cgcatcaaca ctatatatat gacggtgtac tttctgggcg gatcgctcgg ctcacttttc
    11401 tccaccattg cctgggcgca ttggggatgg agcggtgtct gtgccttggg gctcatcatg
    11461 ctggcgctgg ctgcactgat ccacgccacg ggagtgcggg ccggcgagcc cgtttctgtt
    11521 ccttcgtgag gagttattca gaggattggc acctgcgcac aacctggcat ttatttactg
    11581 gccctcatcc ccttcaggaa ttgcataata tcccttgcgg gcctgcacct tcaggccgtc
    11641 gcctttgcag gagatgtcca gcttgcggaa actgccatcc attttgttgt tggtgggcgt
    11701 atagctggcc agatactgtg tccgcagctc atcttcaatc tgtctaaagg cggcttccat
    11761 ctttttgccg ttattgccga catcaatcat gcggcctcct gtttcctccg ccattttctt
    11821 cagggcggag gctccggtgt agcccattcc atagccataa tagaagccgc ggtcggcaat
    11881 cagcagcgca tagatgatgg tgttggattt ctgcgcctct tcaatcgcgt cggcgatttt
    11941 gtgcatgctg ccctggtctt ctccatcggt cagcaaaata agcgccttgc ggccagtctc
    12001 agaacgtagc ttgtcggatg cctgatagac ggcgtcgtac agaagagtgc ctttggggtc
    12061 gccatgtacc gggaccggcc cctgccccat ccccggtacc ccgatcgcgc cgccaccgcc
    12121 gccggtgttg atcaccgcct ggtccatggc cttttccagc agccgcggat tgctggtgta
    12181 gtcctgtaag agactcacct ggacgtcaaa tgagacgaga aatgcctcat ccttagggcg
    12241 caggatgcgc ttgagaaatg ctgcgcctgt ttgctgctcc atcggcagga cattttgctg
    12301 gctgccgctt gtatccagaa gaatgcctaa cgtaagcggc aggtccgttt ccggagcaaa
    12361 atttttgatc ttctgcggtt gcttgtcttc aaagatggcg cagtcatctt ttgtaaggtc
    12421 cgggaccagc ccaccctgtt tgttccggac cgtgaaatag atgctgacga gattgacatt
    12481 ggccttaaag gtaggcgtgg ccgtgttctc ctgctgggta gtatcgggag cattgctcac
    12541 cggaggagca tcaggagagc caggttcctg agcataggaa cgcaccagaa acagcatggc
    12601 cgccataaaa acaaagatgg aactgcgccg cagcatggtt ctactgtatt ggacgggcaa
    12661 cccggaaaaa aggtctatgg cagcgcacag tgcgcagggc acggccggaa accgaaaaac
    12721 gaacatccca gacttacacc tgaagccccg agagctgtca tttggtagtc ttaaagatac
    12781 aacaggtccc cggtggacac tcaccaaacg gggacctcaa gcatctaaga aggaagtgcg
    12841 gttgttctgc tctaattgga tgaacggcag aaggttgcgc gacagaggtt gggggttttt
    12901 ctaaacgatg caaggcgcct tggcagtatt gctggccttt cttatgctgg gtgtgcccca
    12961 gcagcaacaa cagcagagca ttccggacgc gccagcgccc cagcccgcca gctcgtctga
    13021 cctgtctaac ctcaaaggcc agatcgcgcc ggggaaaggc acgtcctctg aagctctgca
    13081 acagtctgct cccgcggccc aacagccagc gcccaaccag cagcagccat ccgcttcgtc
    13141 ttccgatact cagtctgcgc cgcagccaca agaggacaat ggtcagcagc aacaggcccc
    13201 atacatcccc aaaccgggcg aacagcaggc atttttaatc cgcgtgcctg ttacgtatgt
    13261 ggatgttcca gtcacagtgc gcgacaagaa ccatcatctt gttgctggac ttacctggcg
    13321 acagttcaaa atttacgagg acggcgtccg gcagaaaatc gccttcttct cggtggaccc
    13381 gcgtccgctt tccgtcgcct ttgtgattga ccagaccctg ccctccgacg tgatgcagaa
    13441 ggtcaatcag gccctttcag ccgtgaccgg cgctttcacg cctgcggact ccgtcgctgt
    13501 tttcgcttac aacagcagcc cacggatcat taccgagttc accggagcgc agggcgcccg
    13561 cctgccagcg gcgctgcaaa gcgccaaggc ttccggacgc gatatgggtg tgatctctcc
    13621 gggaggcccg ctcgacagcg gccctaccat caatggcaaa ctcgtagacc cgaacgtcgc
    13681 cccgcagcgc ggcaatatgg gctttatcac cattccgaag gaagtccatc cgctcaacga
    13741 cgcgattctg gaagcagcca aggcactggc gcagcaacct aagggaaatc tgcgcgtcct
    13801 ctatgtcatc agcgatggca aggagtccgg cagccaggcg tcctataaag aagtcgtccg
    13861 ttatctgctg accaacaaca tcgtgcttta cggaacactg gtcggcgatt ctgccatgtg
    13921 gggtgtgggg tatctggaca agtggcacat tccgcttgtg cctaccatgc gcgacaacat
    13981 tctgccgaaa tatgcattgg ccaccggagg cgccctcgag tcccagtact cggaaaacgg
    14041 cattcagaag agctttgcca cgatcgcttc ctcggcgcgt acgcagtact tcctgggcta
    14101 ttacacccat tccggcatca tgagcgacaa attccacaag attaccgtcg tcgtcgaagc
    14161 gccggggctg gatataacag caaaggacgg ctactacccc tcggcgtcca tgcgccaatg
    14221 agcggtcccg gtcttgccct gggcgctgcc gccgtctggg gcgcatccga tttctgcggt
    14281 ggactggtca cgc
//
LOCUS       DASRBV010000347         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_24586,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000347 DASRBV010000000
VERSION     DASRBV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24586"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFA02_12935"
     CDS             complement(<1..614)
                     /locus_tag="VFA02_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU10801.1"
                     /translation="MAKKAALSMTYIGGPTALIEFDRLKLITDPAFDPAGGEYVAGPV
                     TLWKTEGPALDAASVGKIDAVLLSHDHHFDNLDRSGRELLASAGQVITTEEGAARLGG
                     NATGLAHWQTIELPGPDGRILRVTGAPARHGPEGGDRGPVIGFVLQFTDDPENAIYVS
                     GDTVWYEGVEEVLRRFPIKIAVLFLGAARVPVSPSHVTFTAEEGV"
     gene            complement(654..1115)
                     /locus_tag="VFA02_12940"
     CDS             complement(654..1115)
                     /locus_tag="VFA02_12940"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HZU10802.1"
                     /translation="MTGRTRSVLKGALAGLVGGLAGAGAKEVAEQIFPPRTEGQTPPP
                     VALAERVTGHPLPPEKQKTAMQGIHWAFGALAGAVYGAAVEFEPSMGAWKGAAFGITL
                     NKLTHESLLPKMGLTAPTKLQPPRERLSEWATHAVYGVVTDAVRRAVRHVL"
     gene            1325..1400
                     /locus_tag="VFA02_12945"
     tRNA            1325..1400
                     /locus_tag="VFA02_12945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1358..1360,aa:Ile,seq:cat)
     gene            complement(1469..2803)
                     /locus_tag="VFA02_12950"
     CDS             complement(1469..2803)
                     /locus_tag="VFA02_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10803.1"
                     /translation="MKKVVFASVLAVTAGAVPFVPAALALDDGQGQNQGAQQGTTITI
                     KDPAEYNAYTNAVGQSSPAAKAAAIEQFLQQYPNSVVKEDMLEQLMAAYQQTGDVNKT
                     LDAAKRLLAVNPNNVRALFFVAYLEKSTANGDQSKLDDAANKAQTALNAPKPERLSDA
                     DYQKLKDTTTPILEDIIGADDVAKKDYKAAIEAYTAELKAYKDPAQTQSGPGLLATYN
                     LGNAYLQQDPKDLVNGIWFLTRAAQFAQPPYKDQIEKAAEYWYKKYHCAANDTNCQNT
                     LDGYAQIQQLAKNNLFPPPDYKPVPAPPPPSPQELAHNAVVSTPDLKTLALGDKEFIL
                     ANGSPEDAGKVWDVLKGQTVQVPGTVISATADSVQLAVSDDAKQSQKADFTINMKTPL
                     KEVPQPNTQVTYIGTFDSYTQNPLMIILKDGEPPAPAKKTPARRPTAHRPRQ"
     gene            3127..3366
                     /locus_tag="VFA02_12955"
     CDS             3127..3366
                     /locus_tag="VFA02_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZU10804.1"
                     /translation="MPENEVKITVRLNGPYRVEGPVKLVDAEGREWDLSGKPAFSLCR
                     CGGSVNKPFCDGTHSKIGFHGAEAAVAKSEGEKQG"
     gene            complement(3495..>4722)
                     /gene="lysS"
                     /locus_tag="VFA02_12960"
     CDS             complement(3495..>4722)
                     /gene="lysS"
                     /locus_tag="VFA02_12960"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZU10805.1"
                     /translation="HIGVTGYLFRTRTGELTVHVKTITFLAKAMIAMPEKYHGVSDVE
                     LRYRQRYVDLFMNSTLNEGGPDEIHVRDVFLKRAKVLRAIRNFFDARGYIEVETPMMQ
                     PIAGGAAARPFVTHHNALDIDLFLRIAPELYLKRLVVGGYDRVYEINRNFRNEGISTQ
                     HNPEFTMLEFYQAYANYHDLMDLTEEIIKHVAQEVNGTAITHFNGHEIDLAKWRRFSM
                     RKAILEFWPEEAGAKPNIADFNSAETTVALVERLRSARIHIEYKPGEPLGKTIATIFE
                     VVAEDHLIQPTIIYDFPLAVSPLSKQKPDEPDWVERFEFYIGGFELGNAFSELNDPEE
                     QRKRFEEQLKQREGGDEEAHQMDEDYVRALAYGLPPTGGEGIGIDRLTMVLTGARSIR
                     DVILFPLLRPQQNAEE"
BASE COUNT          991 a         1279 c         1355 g         1097 t
ORIGIN      
        1 acgccttctt ctgccgtaaa cgtcacatgc gaaggtgaaa ccggcacgcg cgctgcgcca
       61 agaaagagca ccgctatctt gatagggaaa cgcctgagca cttcctccac gccttcgtac
      121 cagaccgtat ctccggaaac ataaatggcg ttttcaggat catctgtaaa ctgcaggaca
      181 aaaccaatca ctggaccgcg gtcgccacct tcagggccat gccgggcagg ggccccggta
      241 acgcggagaa tgcgcccatc cggaccaggc aactcgattg tttgccagtg agcaaggcct
      301 gtcgcattgc cgccaagacg cgctgcgcct tcctcagtcg taattacctg gcccgcagaa
      361 gcaagcagtt cgcgtccgga gcggtcgaga ttgtcaaaat gatggtcgtg gctcagaagg
      421 accgcgtcta tcttgcctac agatgctgca tccagagcag gtccctcagt cttccataac
      481 gtaaccggac cagctacata ttctccgcct gccggatcaa acgcggggtc ggtgatcagc
      541 ttcaatcggt caaactcgat cagagcggtc gggccgccaa tgtaggtcat gctgagagca
      601 gctttcttgg ccatgcagaa aagatgctca ggtaaaacgg ttagattcag cagctataaa
      661 acgtggcgca ctgcgcggcg gacggcatcg gtcacaactc cataaacagc gtgcgtggcc
      721 cattcgctga ggcgctcgcg cggcggctga agtttagtgg gagcagtaag gcccatcttt
      781 ggcaacagcg attcgtgcgt cagcttattg agagtaatgc caaaggccgc gcctttccac
      841 gcacccatgg aaggttcaaa ttctacggct gctccgtaaa cggctccagc aagcgctcca
      901 aaggcccagt gaatgccctg catcgcggtt ttctgcttct ctggaggaag cggatgtcct
      961 gtcacacgtt cagccagagc gaccgggggc ggagtctgac cttccgtgcg tggaggaaaa
     1021 atctgttcgg caacttcttt cgcacctgct cccgctaatc cgccaaccaa tccagccagt
     1081 gcgcccttga gcacagatcg tgttcggcct gtcatattcg ctgagatgct atcaacaata
     1141 tgaaggaaaa cccaggataa aaaggaaaag ccggaccact tcgcggggcc aatcaccctc
     1201 gagtctatga ctctcatcat ttctgtggtt ctggatagcg ccctgctgtg caatttccac
     1261 gacgggcgtc cgctttgagt tacgctaaaa tgaataagcg ccacagcaac atatcttcca
     1321 gaacgggcct gtagctcaac ggtcagagca ggggactcat aatcccttgg ttgggggttc
     1381 gaatccctcc gggcccacca aaatcacctc atccccaaaa gcaggaaagg gcagccgtag
     1441 ctgcctcttc ctgcctgttt gaacagattt actggcgtgg acggtgtgcc gtcggacgcc
     1501 gtgccggagt cttcttggct ggcgccggcg gttcaccatc tttcaagatg atcatcagcg
     1561 ggttctgcgt ataggaatca aaggtcccga tgtaggtcac ctgagtattg ggctgcggca
     1621 cttccttaag cggggtcttc atgttgatgg tgaagtccgc cttttgcgac tgcttggcgt
     1681 catcggaaac cgcaagctgc actgagtcgg cggtagcgga gatgaccgtg cccggaacct
     1741 ggactgtctg gcctttcagc acgtcccata cctttccggc atcttccggt gagccgttgg
     1801 ccaggatgaa ttccttgtcg cccagcgcca gggtcttcag atcaggcgtg ctgacaacag
     1861 cattatgggc gagttcctgc ggagacggcg gcggcggagc aggtaccggt ttgtaatccg
     1921 gcggcgggaa gagattgttt ttggcaagct gctggatctg agcatagcca tcgagcgtgt
     1981 tctggcagtt ggtgtcattt gcggcgcaat ggtacttctt gtaccagtat tcggcagcct
     2041 tctcgatctg gtctttatac ggaggctgcg caaactgggc tgcacgggtg aggaaccaga
     2101 tgccattcac gaggtctttc gggtcctgct ggagataagc attgccgaga ttgtacgttg
     2161 ccagtaatcc agggccggac tgcgtctggg ccgggtcttt gtatgccttc aactcggccg
     2221 tgtatgcctc aatggcggct ttgtagtctt tcttggcaac gtcgtctgcg ccaatgatgt
     2281 cttccaggat aggagtagtt gtgtccttca gcttctgata atcagcatcc gacagccgct
     2341 caggcttagg agcattcaga gcggtctgtg ctttgttcgc cgcgtcgtcg agtttggact
     2401 ggtcaccatt cgcagtgctt ttttcaaggt atgcgacgaa gaaaagcgca cgaacgttgt
     2461 tcgggttgac agccaggagg cgcttggctg catccagtgt tttgttcaca tcgcctgtct
     2521 gttgataggc agccatcaac tgctccagca tgtcttcttt caccacgctg ttgggatact
     2581 gttgcagaaa ctgctcaatc gcagcggcct ttgccgcagg agatgactgg cctacagcat
     2641 tggtgtaggc gttatattcg gcagggtctt taatggtgat ggttgttccc tgctgcgcac
     2701 cctggttctg gccctgacca tcgtccaatg ccagggcagc ggggacaaat ggcactgccc
     2761 cggcagtaac ggccaaaacg gaagcaaata cgaccttctt cattcaacgc tcctaggaaa
     2821 atcctgaaat attgctgatg cggattggag ggtgctccac ttgcatggaa gcatatcatt
     2881 ctttcgtgag agagaaccca atctgtccat gcaatgtgcg aattataaaa atccaccgct
     2941 atgagtgaca agtaaacgct tttgacgctc ccgtcaatcc gaaaatgtga tgcagataca
     3001 gtgcagcgcg ttgacttgct catttatttg taaatgacac cgcagctacg tttagagtgc
     3061 cggggagttt cgtcgggttc ccgctatact caaactcgcg aacaaaattt cttacaggag
     3121 tgtttcatgc cggaaaacga agtgaagatt accgtccgtc tgaatggccc ttaccgtgtg
     3181 gaggggcctg taaagctggt ggatgccgag ggcagagaat gggacctgtc cggaaagccg
     3241 gccttttcgc tttgccgctg tggcgggtca gtgaacaagc ctttttgtga tggtacccac
     3301 tcgaaaattg gctttcacgg agcagaagcc gcagttgcca agtccgaagg tgaaaagcag
     3361 ggatagcata actgcccatc cggccatctg cctgaatggg cacccagcaa agaaaaggcg
     3421 ggtctttcgc gatgttcagg atgacatcaa taatctgtca tcctgaatcc agggtaacgg
     3481 gagtgaaggg cctgctattc ctccgcgttt tgctgcggtc tcagcagcgg gaacagaatc
     3541 acatcacgaa tggaacgcgc tccggtcagg accatcgtca ggcggtcaat gccgatgcct
     3601 tcgcctccgg tggggggcag gccgtaggcc agggcacgga cgtagtcctc gtccatctgg
     3661 tgggcctcct cgtcgccacc ttcgcgttgt ttcaactgtt cttcgaagcg cttgcgctgc
     3721 tcttcggggt cgttcagctc gctgaaggca ttgccaagct cgaacccgcc aatgtagaac
     3781 tcgaagcgct ccacccagtc cggctcatcg ggcttctgct tggagagcgg cgagacggcg
     3841 agagggaagt cgtagatgat ggtgggctgg atgaggtggt cttccgcgac cacttcgaag
     3901 attgtggcga tggtcttgcc gagcggctca cccggcttgt attcaatgtg gattctggcg
     3961 gaacgcagcc gttccaccaa agccactgtg gtttcggcgc tattgaagtc ggcgatgttt
     4021 ggctttgcac cagcctcttc cggccagaac tcaagaatcg ccttccgcat cgagaaccgc
     4081 cgccactttg ccagatcaat ctcatgcccg ttgaagtggg tgatggccgt gccgttcacc
     4141 tcctgcgcga catgcttgat gatctcttcg gtcaggtcca tcaggtcgtg atagtttgca
     4201 tacgcctgat agaactcgag catggtgaat tcagggttgt gttgtgttga gataccttcg
     4261 ttgcggaagt tgcggttaat ttcatacacg cggtcatatc cgccaacgac caggcgcttc
     4321 aggtacagct ccggcgcgat gcgcagaaac aaatcaatgt cgagggcgtt gtggtgcgtg
     4381 acaaatgggc gtgccgccgc tcctccggcg atgggctgca tcatcggcgt ctcaacctcg
     4441 atgtatccgc gcgcatcgaa gaaattgcgg atggccctga gcactttggc gcgcttgagg
     4501 aacacgtcgc gtacgtgaat ttcgtctggc ccgccttcat tcagcgtgct gttcatgaag
     4561 agatctacgt aacgctgccg gtagcgcagc tcgacatctg agacgccgtg gtacttctcc
     4621 ggcatggcaa tcatggcctt ggcgagaaac gtgatggtct tgacgtggac ggtcagttcg
     4681 ccggtgcgcg tgcggaagag gtatcctgtg actccgatgt gg
//
LOCUS       DASRBV010000348        14904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_540198, whole genome shotgun sequence.
ACCESSION   DASRBV010000348 DASRBV010000000
VERSION     DASRBV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540198"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFA02_12965"
     CDS             <1..315
                     /locus_tag="VFA02_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZU10806.1"
                     /translation="GFRASKELREKLDTLHNAAQERRVILIAYTREDGQPSVRRVHPL
                     ALYFWGGTWTLAAWCELRNDFRTFRVDRMQHLDALDEIFTPKAGQMLSDYLRKIRNER
                     PQ"
     gene            complement(332..1564)
                     /locus_tag="VFA02_12970"
     CDS             complement(332..1564)
                     /locus_tag="VFA02_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU10807.1"
                     /translation="MNHMNRRQFVQSISSAAVAGTMLRSAGAKALSANDRVRVGVIGP
                     GSRGQELIRQLLRVPDVEIAAVCDVYEPRFAQVNKLVGRQVPAYKDYRELLARKDIDA
                     VVVSTPVALHAEHVIAAVRSGRPVYGEKALGFTPDDCKNIVAEVEKSGQVFQIGHEYR
                     YAAWVQESIRRIHEGRIGEPTHVYAYWHRNNNWRRPVPQPDPGGKLEHLINWRLYRET
                     SGGLLTELGSHHIDIANWVFGEQPSNVLGTTSIVKYHDGRTVGDNVQAVFKYSKGRRL
                     VFSSLTDNASVGNQLWIYGTEGSVQLTIEDATFFYEPKTHQAVPAQGEVVQRGITTGA
                     SYQTKNEMPYRGPGEKVETPNAEDPTLTAIRSFVQSIRTKQKPLADVRAGYASAIATS
                     IGNQAIAQENALVVPKLS"
     gene            complement(1573..2658)
                     /locus_tag="VFA02_12975"
     CDS             complement(1573..2658)
                     /locus_tag="VFA02_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZU10808.1"
                     /translation="MSAEVVLPELKPRCSILLRRSCAKAWEAIFLLIVSVLISKAVAQ
                     SAPQLFTLTHPAMGTVFTLYIYAASREDADAKANPVFDEIDRLDDLLSNYKETSELSR
                     INRLAAAQTVTTDPETFEFLSDSLQWSARSDGAFDITVGELMKAWGFFRRQGRIPTGA
                     EFAKLREETGWKKVKLDAATRTVRFTSPGIELDPGGIGKGFAVDKAVVVLRSEQVTAA
                     MLSAGGSTVYALGAPPGKTGWKVEVPDPRNPKSVLSVVTLRDTSLSSANCSEKHFILN
                     GHLYCHIMDPKTLRPVEGMLQVTVTDPSATNSDALSNVLFVRRPEENVPLMESLPGDS
                     ALVITGTQQKATCHRMNWKAPVAPGHC"
     gene            complement(2648..3613)
                     /locus_tag="VFA02_12980"
     CDS             complement(2648..3613)
                     /locus_tag="VFA02_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZU10809.1"
                     /translation="MPTSRRAFLAGLAATGVMSRVPVLAQRMKSCPFRLSVINDEISQ
                     DFDHACSVAANDFGLDWIELRGMWNKNISNLNSSEIAEAQRILDKYKLRVTDIASPLY
                     KTDWPGAPVSKFSEHRDQFKASFDFKQQDELLDTCIELAKAFKTDRIRCFDFWRLDDP
                     EKYRAAINDKLREAAEKCAKHNLVLILENEMSCNTATGKEAAAVLKAIPNKNFMLNWD
                     PGNAATFPETTPYPDGYNLLPKDRIGHCHCKDVKRKPDAKYEWAPVGAGVVDWVGQFQ
                     ALKRDGYHYAVSLETHWRGAGTPEASTRISMHGLKDTLKKAGIEC"
     gene            complement(3722..7111)
                     /locus_tag="VFA02_12985"
     CDS             complement(3722..7111)
                     /locus_tag="VFA02_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10810.1"
                     /translation="MTSIRARHILLSAMVILIFGIGPISAGAQAVSATLLGSINDNTG
                     ASIAGAKVTIQEVSTGITHETTTNASGNYTFPDLAPGAYSVTAEATGFKKETRSRVDV
                     IVNTATRVDLALEPGSTTETITVTDVPPILQTDRADVSTKFEARHLEDMPLGVNRNFQ
                     GLLNLVPGTTPASFQHSQFFNAQSSLQTQVNGIPRMGNSYQIEGIDDDERTGLLQIMI
                     PPADAIQTVDISTNNFEAELGRAIGAVTNVTLKSGSNAFHGSAIEYLQNSAFDARSYF
                     SQRVGHVAYNYFGGNFSGPILKDKLFFYGDYYRTADHEANSNTLTIPPREFYTPNANG
                     YIDLSAPLKSDGTGQIYDPATGNPDGSGRTPFPNNQIPINRVNPVSLKLLQLLPAEIP
                     GSSLSSPNNNYFAILPFRKTADTYDAKIDAQLTDKDHLSGRFSYQRVNVFQAPVFGEI
                     AGGPAQGAFEGTGLQNALSTGINYDRAFSSTLLTEARFGIAHYHNNATPAGYGHDYAT
                     QIGVPGVNISQFTSGQVGIDLGSFSSPIIGYSASLPWRRGEANIDFVNHWTKIIGNHT
                     FKFGGDLRRVQDNLLQDQTFSPRGVIQFRENQTSVKGGKTNLANDMASFLLDVPSQVG
                     RDINTYSPAYRQWWLFLFAGDKWQASPKLTLDLGLRWEFYPPATPGKAGGFSNYDPTT
                     STLVIAGVGGNPSNLGMKTRYTYFAPRTGFAFRATDKTVFRGGFGISYMPFPDNTYAY
                     NYPVRANNSYQPLGAYTPAVLDNGQVATFQAGFPAPKPIQIPSNGLLPAPKNQSYVVI
                     PKNYQNPYVESWNLAIEQALAGQFTMNLSYVGNHGVRIGVSQNVNLPPALGLGPTAEP
                     EAIAFGRTAATNVYFLGFSSNYQALQAQLTRRFTNGLATETAFTWGKGLGYQDGDDGG
                     LHFWIDQRHNYAPNDFDRRLNFEESFTYELPFGRGKRWLNRGFTSVAFGGWKLSGIIS
                     IVSGTPFTVTANGGSINTPGQTQTANLVKPFHVLHGIGPGKPWFDITSFTQPVGCTGT
                     PCPVQYGTTLGSSGRNQFYGPGFIQDNISAFKTFPLWERLTLETRVDAFQLSNTPQFG
                     NPSASITSTTFGQVTSTIGSGSGVNGTGGGRALQLSGKLRF"
     gene            7447..9276
                     /locus_tag="VFA02_12990"
     CDS             7447..9276
                     /locus_tag="VFA02_12990"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZU10811.1"
                     /translation="MANTAMERWPNGKFVSDDKPWKWNYELGVLLQGMGDVWYHTGNA
                     AYYKYIKSSVDALVTADGSIPTYKPQDNELDDLALGRTLLLLYGVTQDAKYYKAATLL
                     RDQLKTHPRTSDGGFWHKQRYPNQMWLDGLYMAGPFYADYAVTFQEPQNFADITKQFV
                     LMEQHARDPKSGLLYHGWNETKQQRWANKTTGASPNFWARGMGWYMMALVDTLSYYPE
                     DDPGRAKLLAILQRLAVAVTKYQDKSTGLWYQVVDKPREKGNYFESSAACMFTYALAK
                     GVRRGYLPPSYLQNAERAWQGILKQFVKTDADGKLTLTGTVKAIGLGAGAGNDGSYEY
                     YTTQEVVSNDPKGVGAFLMAATEIESAQTAKLGGGKTVLLDAWYNSQTRKNAAGQTVL
                     YHYKWDDYANSGYSIFGHMLREYGLKTETLTHAPTLADLNKTNIYLIVSPDNAQWNKS
                     PHYMNEADAAVIAEWVRKGGMLVIMQNDPTHADIEHMNILADKFGMHFNNVQLNAETG
                     NHFEMARIDAPADGRVFHSPHVLFMKEICTISPKPPSASVWTWKGNILLAEATYGKGV
                     VVGLVDPWLYNEYTDGRKLPPPYDNFAGGKEYILWLLKQLPKH"
     gene            9306..10136
                     /gene="kduI"
                     /locus_tag="VFA02_12995"
     CDS             9306..10136
                     /gene="kduI"
                     /locus_tag="VFA02_12995"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HZU10812.1"
                     /translation="MKMHLLADPVRYPRMTTAELRESFLLENLYRPGTIQLAYVDLDR
                     AIVGFAAPEASPLPLPTDDTLKAGYFTERRELGALNIGGAGSITVDGKRYDLAHLDCL
                     YIGRGNREISFASDDELKPAAFYLLSYPAHTNFPTTLVKKEDANPTDMGSTETSNKRT
                     IYKYIHLQGARSCQLVMGVTHLASGSVWNTMPPHTHMRRSEIYMYFNLAENARVFHFM
                     GPADETRHFVMRDREIVVSPGWSIHAGAGTSAYSFCWGMGGENQDYADMDPVPVQTLL
                     "
     gene            10219..10686
                     /locus_tag="VFA02_13000"
     CDS             10219..10686
                     /locus_tag="VFA02_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10813.1"
                     /translation="MKYLLLFTTLPLFAIATYAQQQNKADYFSSQDIQKQLSDLTSKA
                     ESSGSSGTTLGDYGSHKIQLSLRTKSGGAEIHAHYDDVFIVKQGHATLTTGGTIPDAK
                     TGPDGETKGTGIIGGQTQEISAGDVVNIPAGTPHQLKIPEGVLFSSVVIKVKE"
     gene            complement(10711..12060)
                     /locus_tag="VFA02_13005"
     CDS             complement(10711..12060)
                     /locus_tag="VFA02_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZU10814.1"
                     /translation="MGQSISRRDMLKGTGASLAALGMGGAIRPVLAQEAESTQAVDQK
                     QEATRAQRMQWWHAAKFGMFIHFGVYSTIGRHEWVMEDEAWPIGPYTGHAKIFKPSKA
                     APRTWAKLAKQTGMKYMVLTTKHHEGFCNFDTKLTNYCAPKQGPGFDIVREYVEAARA
                     EGLHVGFYYSLMDWHHPDGARCAHDEAARRRFVDYTHGLIRELMTNYGKIDVLWYDVA
                     WPLDAAGWESEKMNQMVFELQPEIIVNNRNKLQGDFSTPEQKIVAEKNGRAWEACMTL
                     NDSWGYQRADDNWKSSRTVIRNLVQCVRDGGNYLLNIGPRPDGSVPEESVRILNEVGR
                     WMETNGDTIYKSDLCQVRRSNYASFTRTGNTLYMHVHFWPGEYVAISGLKAKVKSAHL
                     LKTGTEVKFTQDEFQTKFTGLPENAPDYPITTIAIECDSEPLQDTDFVRINKPRDGV"
     gene            complement(12075..12932)
                     /locus_tag="VFA02_13010"
     CDS             complement(12075..12932)
                     /locus_tag="VFA02_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU10815.1"
                     /translation="MNAVAITPSATGINKHADPYQLQSIDRVVAMLEMLSESDVPLSL
                     AEICARMGLHKSTAHRSLIVLERTSLIERTPEGRFRLGLKLYELGNRAVEQIDLRERV
                     QPFFRRLSMEVGETVHLGVLQKTRVVYLDKVEPNRRVCMTSRTGTSNPVYCTSLGKAM
                     LAFLPDEQREDIISKIKFTRFTQKTLCSREALMKSLERVKKRGFAIDDEEIETGVRCV
                     GAPIFDVNRYPIAAVSVSGPASRITVQSVPGIAEKLMRCSADISKTLGIHDKRKSRYT
                     SPFFHHYSN"
     gene            complement(12971..14023)
                     /locus_tag="VFA02_13015"
     CDS             complement(12971..14023)
                     /locus_tag="VFA02_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZU10816.1"
                     /translation="MKALQVMALVTVAVFAVLPMHAAGRLRSPWDGHPVTLTNAPYSC
                     PAIVHLSPDLTTNGFYSDSKSSIIDPEKWKAYAASSGPYKELGNRIVAAADTYRSTGS
                     RAAVECAVGHMEAAAKDGVFTGKMSSNQAYYVQGWTIGAIAIAYLKIRGSGLVSPDQQ
                     QEILHWMTQVVSQSQQYFDAHRTAGKGDAVNNHLYWAGVEVAAAGIAANDRRLFDWGI
                     ATYQVGVAQIQPDGSLPQEMRRGQRALHYHLYALAPLVYLAEFGEVNGLHLYAERDYA
                     LKKLVDLCTHGLEDNSFFVKATGIAQDTPNGAPAAEQISWAKVYLARFQDPELSKLLA
                     QAKSLSYMYLGGLPPG"
     gene            complement(14020..14367)
                     /locus_tag="VFA02_13020"
     CDS             complement(14020..14367)
                     /locus_tag="VFA02_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU10817.1"
                     /translation="MSEIKVIRQDDIAPGTPEAGLQRQVLAWNTKLMLVRHQMEKGWR
                     GALHSHPHDQLVYVIRGRLKFTGNGKSFEIAAGDSFVVPGGIEHQAEALEQSEVLDVF
                     TPYREDYSPEREA"
     gene            complement(14364..>14904)
                     /locus_tag="VFA02_13025"
     CDS             complement(14364..>14904)
                     /locus_tag="VFA02_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU10818.1"
                     /translation="KLFALVSSAMGAPDILVNNAGMIYRNAAEEYEYEAWNTVLQVNL
                     TSVFRLSQFAGKEMLKRGKGKIINIASLLSFQGGIRVPAYAASKGGVAQLTKALANEW
                     AGRGIQVNAIAPGYFRTENTEALQKDETRNRQILERIPAQRWGEPEDLAGAAVFLASA
                     ASDYVTGEVLVVDGGWMAR"
BASE COUNT         3385 a         4307 c         3803 g         3409 t
ORIGIN      
        1 ggttttcgcg cctctaaaga attacgcgaa aaactcgata ctctgcacaa tgccgcgcag
       61 gagcgtcgcg tcatcctcat tgcttacacg cgcgaggacg gccagcccag cgtgcgccgg
      121 gtgcatccac tggccctgta tttctggggc ggaacatgga cgctcgctgc atggtgtgag
      181 ctgcgcaatg acttccgtac gtttcgcgta gaccggatgc agcacctgga cgccctcgac
      241 gaaatcttca ccccgaaagc cggccagatg ttgtcagact atctgcgaaa aatacgcaac
      301 gaacgccctc aataactacg cgagtcatgg tttagctcaa cttcgggact accaaagcgt
      361 tctcctgtgc aatggcctgg tttcctatcg aagtagcaat cgccgaagca tatcctgcgc
      421 gcacatcggc aaggggcttc tgctttgtcc ttatactttg cacaaaagaa cgaattgcgg
      481 tcaacgttgg atcttcggca ttcggagttt ccaccttttc tcccggaccg cgatacggca
      541 tctcgttttt cgtctgataa gaagccccgg ttgtgattcc gcgctgcacg acttctccct
      601 gggccggcac ggcctggtgt gtcttcggct catagaaaaa agttgcatct tcaatcgtga
      661 gctgcacgct gccttctgtg ccgtaaatcc agagctggtt acctacagat gcattgtccg
      721 tcaacgaact gaagaccagc cgtctgcctt tggaatactt gaagaccgcc tgcacgttat
      781 cgcccaccgt gcggccgtcg tggtatttca caatactcgt cgtacccaaa acgttcgacg
      841 gctgttcgcc aaagacccag ttcgcaatat caatgtgatg agatccaagc tccgtgagca
      901 ggccaccgga ggtctcgcgg taaagcctcc aattgatgag gtgctccagc ttgccaccgg
      961 ggtcaggctg tggcacaggc cgcctccagt tgttattacg atgccagtag gcatagacat
     1021 gcgttggctc gccaatgcgc ccttcgtgaa tacgcctgat actttcctgc acccacgcag
     1081 cgtagcgata ttcatgaccg atctgaaaaa cctgtccgct cttttcgacc tcggcaacaa
     1141 tatttttgca gtcatccgga gtaaaaccca gcgccttttc tccataaacc ggccgtccgc
     1201 tgcggactgc cgcaatgaca tgctcagcat gaagcgcaac cggcgtagac accacgaccg
     1261 catctatatc tttgcgcgct aatagttcgc ggtagtcctt gtacgcaggg acctgcctgc
     1321 caaccagctt attcacctgt gcaaaacgtg gctcgtagac atcgcacact gctgcaattt
     1381 ctacatccgg gacgcgcaga agctggcgaa tcaactcctg tccgcggctg cccggaccga
     1441 tgactcctac acgcactcga tcattcgcgg aaagcgcttt cgctccggct gaccgcagca
     1501 tcgtaccagc aacagcagcg ctcgaaatac tctgtacaaa ttgcctgcga ttcatatggt
     1561 tcatgctcta cctcaacagt gtcccggagc aaccggggcc ttccagttca tccgatggca
     1621 agtggccttt tgctgcgttc cggtaatgac cagtgcagaa tccccaggaa gcgactccat
     1681 caaaggcaca ttctcttccg gccttctgac aaacaggaca tttgagagcg catcgctgtt
     1741 tgttgccgat gggtctgtaa ccgtcacctg caacatgcct tctaccgggc gcagcgtctt
     1801 cgggtccata atgtggcagt acaaatgtcc gttcagaatg aaatgctttt ccgaacaatt
     1861 cgcgctcgaa agcgacgtgt ctcttaatgt aactacagaa agcacgcttt tcggattgcg
     1921 cgggtccggc acctctacct tccatccggt ctttcccgga ggcgcgccta aggcatagac
     1981 tgtgctgccg ccagccgaga gcatggccgc agtaacctgc tctgaacgaa gcacgacaac
     2041 ggctttatct acagcaaaac ctttgccgat accacccgga tcaagctcga ttccaggaga
     2101 agtgaatcgc acggtgcgtg ttgctgcatc aagcttcacc ttcttccatc cagtctcctc
     2161 gcgcagcttt gcgaattcag cgcctgtggg aatccgcccc tgcctgcgga aaaatcccca
     2221 ggccttcatc agttcgccta ccgtgatgtc aaacgcgcca tcactccgcg cgctccactg
     2281 gagcgaatca gaaagaaact caaatgtttc cggatccgta gtgaccgttt gtgctgctgc
     2341 aaggcggttt atacgagaaa gctcgctggt ctctttgtag ttgctcagca gatcatcgag
     2401 ccggtcaatc tcgtcgaaca ccggattcgc cttcgcatct gcgtcttccc tgctcgcggc
     2461 ataaatgtac agggtgaaga ctgttcccat cgccgggtgc gtaagggtga aaagctgcgg
     2521 agcggattgc gccacagctt tcgagataag tacgctgacg atgagaagaa aaatagcttc
     2581 ccacgctttt gcgcaactac ggcgcaacag gatggagcac cttggtttca attccggcag
     2641 cactacttca gcactcaatt ccggctttct ttagtgtatc tttcagccca tgcatgctga
     2701 tgcgcgtcga ggcctccgga gtgcctgccc cgcgccagtg cgtctccaga ctcacggcgt
     2761 agtggtagcc atcgcgcttc agcgcctgga actgccctac ccagtccact acgcccgcac
     2821 ctaccggtgc ccattcatac ttcgcgtccg gcttgcgctt tacgtcctta caatggcagt
     2881 gcccgatgcg gtcttttggc agcagattgt agccgtcagg atagggcgtg gtctccggaa
     2941 atgttgccgc attgcccggg tcccagttca gcatgaaatt tttgttcgga atcgccttca
     3001 gaacggcggc agcttctttg cccgtcgccg tattgcacga catttcattc tcaagaatga
     3061 ggaccaaatt gtgctttgcg catttctccg ccgcttcacg cagcttatcg ttgattgccg
     3121 cacggtattt ctcaggatcg tccagtctcc agaaatcaaa gcagcggatg cggtctgttt
     3181 taaacgcctt tgccagctca atgcaggtat caagaagctc gtcctgctgt ttgaaatcaa
     3241 agctggcctt gaactggtcg cggtgctcgg aaaattttga aaccggagca ccaggccagt
     3301 cagtcttata cagtggactt gcgatgtccg tcacgcgcag cttgtattta tcgaggatgc
     3361 gctgcgcttc agcaatctca ctcgaattga gattgctgat gttcttgttc cacattccgc
     3421 gcaactcgat ccagtccagg ccaaaatcat tggctgccac cgaacaggca tgatcgaaat
     3481 cctggctgat ctcatcgtta atgacagaaa gacggaacgg acagcttttc atacgttggg
     3541 ccaaaactgg gacccgtgac atcactcctg tagcggccaa tccggccagg aacgcacggc
     3601 gagacgtggg cattcaacct cctgcattgg gaacaaattt atggtaacgc gtccatgcgg
     3661 gactccgtcg cacggactca ggtgctttca acttttttgt accggatgac ggtattaaaa
     3721 atcagaagcg taattttcca gatagctgca aggccctgcc ccctccggtc ccattcacgc
     3781 cggagccgct gccaatagtt gaagtcacct gaccaaaggt ggtgctggta atcgaagcgc
     3841 tcgggttgcc gaactgcggc gtattgctca actggaatgc atccacgcgc gtctccagcg
     3901 tcaaccgttc ccacaaagga aatgtcttga atgcagagat gttgtcctgg ataaatccag
     3961 gcccataaaa ctgattgcgc cccgagcttc ccaacgtggt cccatactga acggggcaag
     4021 gtgtacctgt gcatccaaca ggctgcgtga agctggtgat atcgaaccag ggtttgccag
     4081 gaccgattcc gtgcagcaca tggaatggct tcaccaggtt cgcggtctgc gtttgtccag
     4141 gagtattgat tgagccgcca ttggccgtca ccgtgaacgg agtgccggac acaatcgaga
     4201 tgatgccgga aagcttccat ccgccaaacg caacggaagt gaagccgcga ttcagccagc
     4261 gtttccctct tccaaacggc aattcgtatg taaagctctc ttcaaagttc aaccggcggt
     4321 caaaatcatt cggagcatag ttatggcgct gatcaatcca gaaatgcaac ccgccatcgt
     4381 caccatcctg atagccaagt cccttgcccc atgtaaatgc tgtctccgtg gccaggccat
     4441 tcgtgaaacg gcgagtcaat tgtgcctgca acgcctgata attcgatgag aatcccagga
     4501 agtacacatt ggtcgccgca gttcgtccaa aggcgatcgc ttccggctca gcggtcggcc
     4561 caagcccaag cgcgggcggc agattcacat tctgcgatac acctatgcgt actccgtgat
     4621 tgcccacata ggacaaattc atcgtgaact gtccggcaag cgcttgctca atcgccaggt
     4681 tccacgattc aacataggga ttttgataat ttttcggaat cacaacataa gactggtttt
     4741 taggtgcagg cagcaaccca ttggaaggga tctggattgg tttgggcgct ggaaatccag
     4801 cctgaaatgt tgccacctgg ccattgtcca gcaccgcagg agtatatgcg ccaagcggtt
     4861 gatagctatt gttcgcccgc accggatagt tatacgcata cgtgttatca ggaaacggca
     4921 tgtagctgat accgaatccg ccgcgaaata cagtcttgtc tgttgcgcga aaggcgaatc
     4981 ccgtgcgcgg tgcaaagtag gtataacgtg tcttcatgcc caggttcgac ggattgccgc
     5041 ctacccctgc aatgacaagc gtgctcgtgg tggggtcgta attcgagaaa ccaccagctt
     5101 tgcccggtgt cgcaggcggg tagaactccc agcgcagacc caggtccagt gtaagtttcg
     5161 gagatgcctg ccatttatct ccggcaaaaa gaaatagcca ccactgacgg tatgcgggcg
     5221 aataggtgtt gatgtcgcgc cccacctggc tgggcacatc cagcagaaag ctggccatgt
     5281 catttgcaag gttcgttttt ccgcctttta cagaagtctg attttcgcgg aactgtatca
     5341 ctccgcgcgg actgaatgtc tggtcctgga gcaggttgtc ctgcacgcgg cgcaggtccc
     5401 cgccaaactt gaatgtgtga ttgccaatga tcttcgtcca gtggtttaca aaatcaatgt
     5461 tggcctcacc gcgcctccac ggcagcgacg ctgaataacc gatgatcggg ctgctgaaag
     5521 agccaagatc aatccctacc tgaccgctgg tgaactggct gatgttcaca cccgggacac
     5581 caatttgagt agcatagtcg tgcccatatc ccgcaggcgt cgcattattg tgataatgtg
     5641 ctataccgaa gcgggcctca gtgagcaaag ttgaagaaaa tgcgcggtca taattgattc
     5701 ccgtgctgag cgcattttgc agacctgttc cctcaaatgc tccctgcgcc gggccaccgg
     5761 caatctctcc aaaaacaggc gcctgaaata cattcacacg ctgatagctg aagcgcccgc
     5821 tgagatggtc tttgtccgta agctgtgcgt caatttttgc gtcgtatgta tcagccgtct
     5881 ttcggaatgg aagaatggcg aagtagttgt tgtttggtga agacagtgaa ctgccgggaa
     5941 tctccgcagg cagaagttgc agcagcttca gcgaaactgg gtttacgcgg ttgatgggaa
     6001 tctgattgtt cgggaatggt gttctcccac tgccatcagg atttccagtc gcaggatcat
     6061 aaatctgccc cgttccatca ctcttaaggg gcgcactcag gtcaatatag ccattcgcat
     6121 tcggcgtata gaactcgcgc ggcggaatcg tcagcgtgtt ggaattcgcc tcgtggtccg
     6181 ctgtccggta atagtctcca taaaagaaca gcttgtcttt gagaatgggg ccgctgaagt
     6241 ttccaccaaa gtagttatag gccacatggc ccaccctctg gctgaaataa gaccgcgcgt
     6301 caaaggcgct gttctgcagg tattcaatcg ctgatccgtg aaatgcattg gatcccgatt
     6361 tcaaggtaac gtttgttacc gctccaatcg cacgccccag ttcggcctca aagttattgg
     6421 tcgaaatgtc cacagtctgg atcgcgtccg cgggaggaat catgatctgg agcagacccg
     6481 tgcgctcatc atcatcaatg ccttcgatct ggtagctgtt gcccatgcgt ggaataccgt
     6541 tgacctgggt ctgaagcgag ctttgtgcat tgaagaactg cgaatgctgg aagcttgcgg
     6601 gtgtggtccc gggcactaga ttcagcagtc cctgaaaatt acggttcact cccagaggca
     6661 tgtcttccaa atggcgggct tcaaattttg tgctcacgtc ggcgcggtct gtctgcagaa
     6721 tcggcggaac gtccgtgacc gtaatcgtct ctgttgtact gcccggttcc agagcgaggt
     6781 ccacacgcgt cgccgtgttc acaattacgt ctacccgcga gcgtgtctct ttcttaaagc
     6841 ccgtcgcttc cgcggtaacg gaatacgcgc ccggggccag gtcaggaaac gtatagtttc
     6901 cgctcgcatt ggtcgtggtt tcgtgagtga ttcccgtgga aacttcctgg atggtaactt
     6961 tcgccccggc gatgctcgct ccggtgttgt cgttaatgct tcccagaagc gttgctgaaa
     7021 cggcctgagc acctgctgag attggaccaa tcccgaatat taggatgacc atggccgaga
     7081 gcaatatatg gcgcgcgcga atggatgtca tgaccccctc ctcaaaagaa ctgcataggt
     7141 ttttcttcct tcgccagatc gggcctgccc gaatttcaca gcgcgaaggt aaatgattgg
     7201 aaacgttgcc aaaactaatg accaaccaca aggattgtca agccattcag aaacaaaacc
     7261 ggagtctcag atagcgagac gcgatccgcg gccatttgca gacatttact acgtctgttt
     7321 gatacacaat ctctaaaccg gacgttgccg atgcgcacta gacacatttc tcttttgctt
     7381 cttttcctgc ttaccagggt ctcgctcttc tcctgtcctg ctcaggagct tccctggtca
     7441 cagcgcatgg caaacaccgc catggagcgc tggccgaatg gaaaatttgt ctctgatgac
     7501 aaaccctgga agtggaatta tgaacttggc gtgctactcc agggcatggg cgacgtctgg
     7561 tatcacaccg gaaatgcggc ctattacaaa tacatcaaaa gctcagtgga tgctctcgtc
     7621 actgccgacg gttccatccc tacttacaag cctcaagaca atgagctgga tgacctcgcg
     7681 ctgggacgca cgctactgct tctttacgga gtaacccagg acgcgaagta ttacaaggct
     7741 gcaacattgc tccgcgacca actcaagaca catccgcgca cttctgacgg cggcttctgg
     7801 cacaagcaac ggtatcccaa ccagatgtgg cttgacggcc tttacatggc cgggcctttc
     7861 tatgctgatt atgccgtcac ctttcaggag ccgcaaaatt ttgcggacat cacgaagcag
     7921 ttcgttctga tggagcagca cgcgcgcgat ccgaagtccg gcctgctcta tcacggatgg
     7981 aacgagacga aacagcagcg ctgggccaac aaaaccaccg gagcatctcc gaacttctgg
     8041 gcgcgaggca tgggctggta catgatggcg ctggtggaca cgctgtccta ctatccggaa
     8101 gacgatccag ggcgcgcaaa gctgctcgcc attctgcaaa ggctcgctgt agcggtaacg
     8161 aaatatcagg acaaaagcac agggctctgg tatcaggtag tagacaagcc gcgagaaaaa
     8221 gggaattatt ttgagtcctc cgctgcctgc atgtttacgt acgcgctggc aaagggcgtg
     8281 cggcgtggct atttgccacc gtcctattta caaaatgcgg agcgtgcgtg gcagggcatc
     8341 ctgaagcagt ttgtaaagac tgatgcagac ggcaaactta cgcttaccgg aacggtcaaa
     8401 gcgattggtt tgggagcagg cgcaggcaat gacggcagct atgagtacta cactacacag
     8461 gaagtcgtca gcaatgatcc aaaaggcgta ggcgcatttc tgatggcagc tacagagatt
     8521 gagtctgccc agactgcgaa gctggggggc ggcaagaccg tcctgctcga cgcctggtac
     8581 aacagccaga cccgcaaaaa cgccgctgga caaaccgtgc tctaccacta caaatgggac
     8641 gactacgcca acagcggcta ctccattttc gggcacatgc tgcgcgagta cggtctgaag
     8701 acagaaacac tcactcatgc acccacactt gcagacctga acaaaacaaa tatctatctc
     8761 attgtttcgc cggacaatgc gcagtggaac aaaagtccgc actacatgaa tgaagccgac
     8821 gccgcagtga ttgctgaatg ggtgcgtaag ggcgggatgt tggtcattat gcagaatgat
     8881 ccaacgcacg cagatatcga gcacatgaac atccttgccg acaagtttgg gatgcacttc
     8941 aacaacgtgc aactcaatgc cgagaccgga aaccatttcg aaatggcacg gattgatgct
     9001 ccggcagatg gacgggtgtt ccactctccg catgtgttgt tcatgaaaga gatctgcacc
     9061 atctcgccaa agcctccctc tgcctcagta tggacgtgga aagggaacat cctgcttgca
     9121 gaagcaacat acggcaaggg cgttgtagtt gggcttgtag atccgtggct atataacgag
     9181 tacacagatg gaaggaaact tccaccacct tatgacaatt ttgcgggcgg caaggaatac
     9241 attctctggc tgctgaaaca gcttccaaaa cattaattga tttttttacc gcacaggaca
     9301 cattcatgaa gatgcacttg cttgccgatc ccgttcgata tccacgcatg actaccgctg
     9361 agctgcgcga gagttttctg cttgaaaacc tttaccggcc cggaacgatt cagcttgcgt
     9421 atgttgacct cgaccgcgcc atagtgggat ttgctgcgcc cgaagcctct cccctgccgt
     9481 tgccaaccga tgacaccctg aaagccggct actttaccga gcgccgcgag ttgggcgcgc
     9541 tgaacattgg cggcgctgga agcattacag tggatggcaa acggtacgat ctcgcccatc
     9601 tcgattgcct ctacatcggc agaggaaacc gcgagatcag ctttgcttcc gatgacgaat
     9661 tgaagccagc ggccttctat ctgctgagtt atccggccca cacaaatttt ccaaccacgc
     9721 tcgtgaaaaa agaagacgcc aacccaacag atatgggcag cacagagacc agcaacaagc
     9781 ggaccatcta taagtacatc catcttcagg gcgcgcggag ttgccagctt gtcatggggg
     9841 tcacgcatct ggcaagcggg agcgtatgga acacgatgcc tccgcacacg cacatgcgcc
     9901 gctcagaaat ctatatgtac ttcaaccttg cggaaaacgc gcgcgtcttc cactttatgg
     9961 gtccagccga cgagacccgg cacttcgtca tgcgcgaccg cgaaattgtc gtctcaccgg
    10021 gatggtctat tcacgcgggc gcaggtacca gcgcttacag cttctgctgg ggcatgggtg
    10081 gggaaaacca ggactatgct gacatggacc cggtccctgt gcaaactctg ctatagaaac
    10141 ggcctccatt tgtaaaatat ccttcgcaaa gcgaggggtg ggagacaaca attttccgct
    10201 tcttaaagga aatcacagat gaaatacctt ctactattta ccacgcttcc cttgttcgct
    10261 atcgccactt acgcgcagca acaaaacaaa gctgactact tctcctcgca ggacatccag
    10321 aaacaactct ctgatttgac atcgaaggcc gaatcttcag gcagcagcgg cacgacactc
    10381 ggcgattacg gaagccacaa gatacaactc tcgctgcgca caaagagcgg tggagcggaa
    10441 atccacgcgc actacgatga tgtctttatc gtgaagcagg gtcacgccac acttacgacg
    10501 ggcggtacca ttccggacgc aaagaccgga cccgacggag aaactaaagg cacaggcatt
    10561 atcggcggac aaacacagga aatctccgcg ggcgatgttg tcaacatccc cgcaggtact
    10621 ccacatcagt tgaaaattcc ggaaggagtg ttgttcagct ccgtcgttat caaggtgaag
    10681 gaatagcgcg gctaccggcg attttgctca ttacacacca tcacgcggct tgttgatgcg
    10741 tacaaagtcc gtgtcctgca gcggctcaga gtcgcactca atcgcaattg tagtgatagg
    10801 atagtccggc gcattttccg gaagtccggt aaactttgtc tggaattcgt cctgcgtaaa
    10861 tttcacttct gttccggtct tgagcagatg cgctgatttc acctttgcct tcaggccgct
    10921 gatggccaca tactcgcccg gccagaaatg cacgtgcata tagagagtgt tcccggtgcg
    10981 cgtaaagctg gcgtaattcg agcggcgcac ctggcaaagg tctgacttgt aaatcgtgtc
    11041 tccgttcgtc tccatccatc gccccacttc attcagaatc cgcacggact cttccgggac
    11101 agagccgtcc ggtctggggc caatgttcag caaataattt ccaccatcgc gcacacactg
    11161 gaccaaattg cgaatcaccg tgcgcgagga cttccagtta tcatccgcgc gctgataccc
    11221 ccagctatcg ttcagcgtca tgcacgcttc ccacgcgcgg ccgttcttct ccgccacaat
    11281 cttctgctcc ggcgtggaga agtctccctg cagcttattg cgattgttga cgatgatctc
    11341 cggctgaagc tcaaagacca tctggttcat cttctctgat tcccaacctg cagcatcgag
    11401 cggccatgca acgtcgtacc acagcacgtc aatcttgccg tagttggtca tcaattcgcg
    11461 aatcagcccg tgggtgtaat ccacaaagcg gcgacgtgca gcctcgtcat gagcgcagcg
    11521 cgcgccgtcc ggatggtgcc agtccatcag cgagtaatag aacccgacat gcagcccttc
    11581 ggcccgcgcc gcctccacat attcgcggac aatatcaaag cctggcccct gttttggcgc
    11641 gcagtaattc gtcagcttcg tatcaaaatt gcagaagcct tcatggtgct tggtggtgag
    11701 caccatatat ttcataccag tctgtttagc cagcttcgcc catgtacgcg gagcagcttt
    11761 tgaaggtttg aagattttag cgtgccccgt gtaagggcca atgggccatg cctcgtcttc
    11821 cattacccac tcgtggcggc cgattgtgct gtaaacgcca aagtgaataa acatgccgaa
    11881 ctttgccgca tgccaccact gcattctttg tgcgcgcgta gcttcctgtt tctgatcaac
    11941 ggcctgcgta gactctgctt cctgcgccag caccggacga attgcaccgc ccatgcccaa
    12001 agcagccaga gacgcgcctg tgcccttcag catgtcgcgc cgcgaaatgg attggcccat
    12061 tttttctctc cccttcagtt gctgtaatga tggaaaaacg gcgatgtata tcttgacttt
    12121 cttttgtcat gaatgccgag cgtctttgag atatctgccg aacaacgcat cagcttttcg
    12181 gcaatccccg gcacactctg cactgtaatg cgcgatgcag gcccggaaac gctgactgcc
    12241 gcgatgggat agcggttgac atcaaaaatc ggcgcgccca cacagcgcac accggtctcg
    12301 atctcttcat cgtcaatggc aaagccgcgc ttttttaccc gttccagcga cttcatcaac
    12361 gcctctcgcg agcaaagtgt cttctgcgta aagcgcgtga atttaatctt gctgatgatg
    12421 tcctcgcgct gctcatcggg cagaaacgcc agcatggcct ttcccagtga agtgcagtac
    12481 accggattcg atgtcccggt ccgcgaggtc atgcacaccc ggcggttcgg ttctactttg
    12541 tccaggtaga caacgcgcgt cttctgcaac acgcccagat gcacggtctc acccacttcc
    12601 atcgagagcc gccggaagaa cggctgcaca cgctcgcgca gatcaatctg ctccacggcc
    12661 ctgttgccca gctcgtaaag tttcagcccc aggcggaagc ggccttccgg cgtgcgctca
    12721 atcagcgaag tgcgctcaag cacaatcagc gagcggtgcg ccgtgctctt atgcagaccc
    12781 atgcgcgcgc agatttccgc aaggctcagt ggcacatcgc tctcgctcag catctcaagc
    12841 atggccacca cgcgatcaat gctctgtaat tgatatgggt cagcatgttt gttgattccc
    12901 gtagcagatg gcgtaattgc taccgcattc atagctcctc cttctgctga tcgcagaggt
    12961 ctactcctga tcagccgggc ggcagtccgc ccaggtacat atagctgaga gattttgcct
    13021 gagcgagcag tttcgacaac tcagggtcct gaaaccgggc aagatacacc tttgcccaac
    13081 tgatttgttc cgcggcaggt gcgccatttg gcgtgtcctg tgcgatgcct gtcgctttta
    13141 caaaaaatga attgtcttcc agaccatgcg tgcagaggtc cacgagcttt ttgagcgcat
    13201 aatcacgctc cgcatacagg tgcagtccat ttacctcgcc aaattcagcc agataaacca
    13261 gcggtgcaag cgcatacagg tgatagtgga gcgcgcgttg accacggcgc atctcctgcg
    13321 gaagcgatcc atccggctgt atctgcgcaa cgccaacctg gtatgtagcg atgccccagt
    13381 caaagagcct gcggtcattc gcggcaatac ctgctgccgc aacttccaca ccggcccagt
    13441 aaaggtgatt gttgaccgca tcgccttttc ctgctgttcg atgagcatcg aaatactgct
    13501 ggctttgcga gaccacctgc gtcatccagt gcaaaatctc ctgctgctga tccggagaaa
    13561 ccagtccgct gccgcgtatc ttcagataag cgatggcaat cgcgccaatc gtccagccct
    13621 gcacataata ggcctggttg gatgacattt tcccggtaaa cacgccgtcc ttcgctgctg
    13681 cttccatgtg ccctacggcg cattccacag cagcgcggct tcccgttgag cgatatgtat
    13741 cagcagcagc tacaattctg tttcccagtt ctttatacgg gcctgaagac gctgcgtacg
    13801 ccttccactt ttccggatca atgatggacg acttgctgtc agaataaaaa ccattcgtcg
    13861 tcagatcagg agaaagatgc acgatagcag ggcaggaata aggcgcgttt gtcagtgtga
    13921 ccggatgtcc gtcccacgga gagcgaagcc gcccggcagc gtgcattggc agtaccgcaa
    13981 agactgcgac cgtcaccagc gccatcacct gtagggcctt catgcttccc gctccggtga
    14041 gtaatcttca cgataaggag tgaatacatc aagcacttcg gattgctcca gcgcctcggc
    14101 ctggtgctca ataccacctg ggaccacgaa gctgtctccc gctgcaattt caaaactttt
    14161 gccgttgccc gtgaacttaa gccgccccct gatgacatac accaactggt catgcggatg
    14221 gctgtgcagc gctccccgcc agcctttttc catctggtgc cgcaccagca tcagcttcgt
    14281 attccaggcc agcacctgcc gctgaagccc tgcctctggc gtgccggggg caatatcatc
    14341 ctggcgaatg actttaatct cactcatcgc gccatccatc cgccatccac caccagcact
    14401 tcgccagtta cataatcgct tgcagcggaa gccagaaaca ccgcggcccc ggcaaggtct
    14461 tccggctcgc cccaacgctg cgccggaatt cgctcaagga tctgacggtt gcgcgtttca
    14521 tctttctgca gggcctccgt gttttctgtc ctgaaatacc ccggcgcaat cgcattcacc
    14581 tggatgccac gccctgccca ttcgtttgca agcgctttcg taagctgggc cactccgccc
    14641 ttggaggccg cataggcagg cacgcgaatt ccaccctgaa aagacagcaa cgaagctatg
    14701 ttgatgattt ttcctttgcc gcgcttcagc atctcttttc cggcaaactg agaaagccgg
    14761 aaaacgctgg tcaggttcac ctgaagcaca gtgttccacg cttcgtattc atactcttcc
    14821 gcggcattgc ggtagatcat tccggcgttg ttcaccagaa tgtccggcgc gcccattgca
    14881 gatgaaacca gggcaaagag cttt
//
LOCUS       DASRBV010000349        11458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_279586, whole genome shotgun sequence.
ACCESSION   DASRBV010000349 DASRBV010000000
VERSION     DASRBV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279586"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..923
                     /gene="lipA"
                     /locus_tag="VFA02_13030"
     CDS             9..923
                     /gene="lipA"
                     /locus_tag="VFA02_13030"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZU10819.1"
                     /translation="MATAAELIQIDLAPRKPAPKPEWLKARAPMGDNYHDLKKLARSL
                     NLHTVCESAHCPNIGECWNHRTATFMMLGNTCTRRCGFCAVPKGRPDAIDFDEPRRVA
                     EAVATLGLKFAVITSVNRDDDIVGGARAFAMVIDEIRKQAPGCQVEVLIPDFQGNEEA
                     IRTVVEARPEILNHNTETVPRLYRAVRSGARYERTLRLLEYSKELDSTITTKSGVMVG
                     LGEEMDELLQVYRDLAAVGTDVLTIGQYLRPSKDHLPIARYYTPEEFAFMKQEALRMG
                     FRHVESGPLVRSSYHAHEQANASGVVLQ"
     gene            complement(1972..3522)
                     /gene="guaA"
                     /locus_tag="VFA02_13035"
     CDS             complement(1972..3522)
                     /gene="guaA"
                     /locus_tag="VFA02_13035"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZU10820.1"
                     /translation="MDTSSIVILDFGSQYTQLIARRIREQNVFSVVLPCTARLEEIES
                     YKPLGIILSGGPSSVYDSDAPPADPAVLRLGKPVLGICYGLQFITHHLGGKVRSAEKR
                     EYGHAEVSVVDPATPLFAGLPSSLQVWMSHGDEALDLPPGFHLIAKTSNAVAGIAHPE
                     KNIWAVQFHPEVHHTPRGAELLRNFLFNICKAKPDWTPEHFIQSTVSAIRQKVGDGHA
                     ICALSGGVDSSVAAVLVHRAIGDRLTCVFVDNGVLRKNEFFKVQENLRGKLGLNLVAV
                     DASGRFLAKLAGVTDPEAKRKIIGNEFIAVFDDEAHRIAQQTGGVDWLVQGTLYPDVI
                     ESSSVKGPSQTIKSHHNVGGLPETMKLKLIEPLRDLFKDEVRRIGRDLRMPDDILQRQ
                     PFPGPGLAVRILGEITPERVALLQEADDIVVSEIKAAGLYQQIWQSFAVLLPVKSVGV
                     MGDQRTYAYTCAIRAVHSEDGMTADWVPLPYDVLKRISSRIVNEIRGINRVVYDITSK
                     PPGTIEWE"
     gene            3588..4604
                     /locus_tag="VFA02_13040"
     CDS             3588..4604
                     /locus_tag="VFA02_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10821.1"
                     /translation="MRTMGFTAIWQVNRISEYHWIKFVFGDLISEEIFDGKHEVVTDH
                     CFVVDQYLHAKPAAYYRSFQGKEAFLIHLSDETYEGGYEHYQHFRGVFRNYWSAVFHP
                     DHVMTFPLGFTHLPLNPDNLPKAQERNYVWSFLGQANKASRPEMLQAFRPVGPQFTYI
                     TDGQDQQQAPLSREEYQRVLLQSVFVPCPMGNVNLECWRIYEALECGAIPIVERRATL
                     DYFGNLLGPNPLPAFRKWSEARDFVQKMTTDPGRLEDFRTQCVAWWEQYKLDLKGRVR
                     DFTQKPQTASPKSVVSGVYRIPGWHGLELLRHHSLGAMQKRISLQAHRLFVQRRLRVT
                     AGRG"
     gene            complement(4545..5486)
                     /locus_tag="VFA02_13045"
     CDS             complement(4545..5486)
                     /locus_tag="VFA02_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10822.1"
                     /translation="MDATQQVRGTQSLVHTIGGCWKRPSLLLLEVAWRWLFGVPALAL
                     LGYELWRVYSATASRLEATGISQFSLQDPMRAAVIIADAFAVLWPPVLHVLLWLAPLL
                     AVGWAIASGLGRDFVLRRYDAALPKRPGSLIVLQLLRVIALAGTFTGWFAAVHWAANY
                     SLSGTDPGLVLYFALVISLSLGIFALWALLSWVFSIAPLLVLLENRSVSSSLVRSLRL
                     GPLTGKLVEVNLVMGIIKLALIVLAMVFSASPLPFATVMQGTELYAWWAVVSLLYLAA
                     SDFFQVVRLVAFIQFWQISSASGSHAQSSLHKQTVRL"
     gene            complement(5487..8114)
                     /gene="leuS"
                     /locus_tag="VFA02_13050"
     CDS             complement(5487..8114)
                     /gene="leuS"
                     /locus_tag="VFA02_13050"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZU10823.1"
                     /translation="MSSSKPETAAAPAVKEYRYNPAEIEPRWQERWAAQPGLYAVEPA
                     SSGKPKYYVLEMLPYPSGQLHMGHVRNYSIGDALARYMWMQGHNVLHPMGWDAFGLPA
                     ENAALKNNTPPREWTLHNIAAMKRQMQRIGFSYDWRNEVTTCLPDYYRWNQWFFIRMF
                     EKGLAYRKKSKVNWCPECATVLANEQVINGRCWRHEDTIVEQRDLEQWFLRITNYAQE
                     LLDDLEKLDGWPEKVRTMQRNWIGRSEGAEVDFAVEDESSAPDDEQSQRKKGTAILPA
                     RENAKTAGKITVFTTRIDTIFGATSIQLAPEHPLVKEFAATDLKLAEEVSSLLDQQKK
                     ARESGDIGEIEKHGVFTGHYAINPFNGERLPIWVANYILAEYGTGAIMSVPAHDERDY
                     DFAKKYGLEIRVVILPRRNGEPDEDGKEEAMLPFTSEDSLLINSGEFNTLGCREAQHK
                     MAAYAQEKGFGKPTVTFRLKDWGVSRQRYWGTPIPMLYCEKDGIVPVPDDQLPVLLPE
                     NVEITQQGGSPLGKLPEFVNATCPKCGGPARRETDTMDTFVDSSWYFYRYTSAKFDKG
                     PFDVTTAAYWFPIDQYIGGVEHAILHLIYSRFWTKVMRDLGLIQNDEPVTRLFTQGMV
                     IKNGAKMSKSKGNVVSPDDMVARYGADATRMYALFAAPPDRDLDWQEDGVAGVSRFLG
                     KVYRFVMRYAGTARSVTATDTIAADAPSQALLRKLHQTIGKITEDFAGRWHFNTCIAA
                     IMELVNTLTAAEEEISAGKVPAGVVKTLLENLVLLLAPFAPYLAAELWEQLGKEQSIL
                     RTPWPKFDEALAREDEIEIPVQVNGKLRAVVKVAAGADQDSMREAVKSDTKVQTATAG
                     KEIVKVIVVPGKLVNIVVK"
     gene            complement(8164..8439)
                     /locus_tag="VFA02_13055"
     CDS             complement(8164..8439)
                     /locus_tag="VFA02_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZU10824.1"
                     /translation="MKMLNPVHPGRILKLSLGSMDISEAAARLKIARPTLSKIINGRA
                     GISADMALRLEAFFGNPSEFWMDLQKQYELAQARRRKRPKIQPMKAA"
     gene            complement(8452..8739)
                     /locus_tag="VFA02_13060"
     CDS             complement(8452..8739)
                     /locus_tag="VFA02_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZU10825.1"
                     /translation="MIISFRHKGLKAFFETESRRGIHPGHAEKLRERLTVLNVIKRVS
                     DVPGNLAVAWNLHPLHGKLEGHYGLSVGGNWRITFTLKEEDVALVDYQDYH"
     gene            complement(8772..9650)
                     /locus_tag="VFA02_13065"
     CDS             complement(8772..9650)
                     /locus_tag="VFA02_13065"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZU10826.1"
                     /translation="MKKNILRIGIHLSTSGGVYMAAERAQAMGANTVQIFSSSPRMWR
                     PARIEPAHCERMRELRQKYGCWPLVIHTSYLVNLCSQSEEVRAKSIAAFHGEVERALA
                     LGAEYLVLHPGSWRGLTREEGLRLAVESIQKALEGLDWQARHFQILIENTAGAEFSLG
                     GSFEQVAVLMHRLRNVAPVGACLDTCHTHVAGYDIVTPEGYQETVRQIEATIGLDHVL
                     VWHMNDARAARGSKLDRHEHIGEGTIGAAAFRRLLQDTRFAHCAFIAETPLDQPGDDE
                     RNVAKLRSLAASVSHS"
     gene            9705..10853
                     /gene="nagA"
                     /locus_tag="VFA02_13070"
     CDS             9705..10853
                     /gene="nagA"
                     /locus_tag="VFA02_13070"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZU10827.1"
                     /translation="MRKIITAEKLFSPEDTVLHPIVVVEDRTITSIVSREEGLFPEGE
                     RYDFPGAILVPAYFDVHFHGSAGSDVMEATEGALNTIGSFLARHGVGSYFATTISAPV
                     DATLKSLSGLAKLMGKDLKGARPLGIHIEGPFLSHAKKGAHAERDLQQPSAGLFNRMW
                     EAAEGHIRLMTIAPELPGAIEVIERAVSLGVRVSLGHSNASSEDALRGVQAGATSATH
                     TFNAMRRFDHRDPGILGVVLDRNDLFAEIICDGLHVHPTVVRMFWKEKGAARAILITD
                     AMSATGMPDGNYKLGELDVRVKNGKCIIGEDTLAGSTLTLDRGVRNFAEFTGVDIATA
                     ATLATRNPARMAGLEPQAGTLAAGRPADITVLSPKGEVIETILRGERG"
     gene            complement(10876..>11458)
                     /locus_tag="VFA02_13075"
     CDS             complement(10876..>11458)
                     /locus_tag="VFA02_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZU10828.1"
                     /translation="AHSRDAQGVSALMLAVYTHQSVVRDFLLTHCTELDIFEAAAVGD
                     CARLQALIAGDAMQVRAVSPDGWTPLHLAAAFAGPEAVTLLVEHGAHAHQVSHNPLRN
                     QPLHACVALNNSVDAVRALLEAGADVNATQHGGYTPLIQAAAAGKKEMVQVLVECGAR
                     LDAKCDQGKTALDYARERGHAEIVKLLEKSLHG"
BASE COUNT         2502 a         3234 c         3287 g         2435 t
ORIGIN      
        1 aggttgccat ggccacagca gccgaactta tacagattga cctcgctccg cgcaagccag
       61 cgcctaagcc ggaatggctg aaggcccgcg cgccgatggg cgacaactac cacgacctca
      121 agaagctggc gcgcagcctg aatctgcata cagtatgcga atcggcgcac tgccccaata
      181 tcggcgagtg ctggaaccac cgcacggcca ccttcatgat gctgggcaac acctgcacgc
      241 ggcgctgtgg gttctgcgcc gttcccaaag gccgccccga cgcaattgac tttgacgagc
      301 ccaggcgagt agcggaagca gttgccactt taggactgaa gtttgctgtc atcaccagcg
      361 tcaatcgtga tgacgacatt gtgggcggcg cgcgcgcctt tgctatggtg attgatgaga
      421 tccgcaagca ggcgcccgga tgtcaggtgg aagtgctcat ccccgacttt cagggcaatg
      481 aagaagcgat tcgcaccgtt gtcgaagccc gtccagaaat cctgaaccac aatacggaaa
      541 ctgtaccgcg cctgtatcgg gctgtacgtt ccggcgcgcg ctatgagcgc acgctgcgcc
      601 tgctggaata ttcgaaagag cttgattcca cgatcaccac aaagtcaggc gtaatggttg
      661 gcctgggcga agagatggat gagttgttac aggtctatcg cgacctggcc gccgttggca
      721 ccgatgtgct tacaattggc cagtatctgc gcccctcgaa ggaccatctg ccgattgccc
      781 gctactacac tccggaggag ttcgcattca tgaagcagga agccctcagg atggggttcc
      841 gtcatgtgga atcaggcccg ctggtgcggt cgtcatacca cgcgcatgag caggcgaatg
      901 cctctggcgt tgtgctccaa tgaactttgc cggggaactg ttgctttctt caggattctt
      961 ccttcgttcc ttcggtcaga taaagggggg tacccccctg tttttgtacg ggaatccttt
     1021 attttcaata agttacaagt aggttcttgt acggagatgc tttgttttca atgagttagc
     1081 gaagaagcgg gtggcgaaga aacgggtagc tgaaaggcgg gtggctgaaa agcggtcagc
     1141 gaagaagcgg tcagcagtca gcgcctgctt cgcaggctgt cagcccagag ccttcccatg
     1201 gtgaacccgg cctccataga taaaccaagt atccataaaa gtcggggcta attttttgta
     1261 aattctagaa aatattatag ttactaaatt tatgctctca ttttcgaggc agtttatacc
     1321 tagattgtct actgatggtt ctgatccttc ataagggatt ccatggggca cccggttaac
     1381 tcagggagct atgagaggcg atctctcagc ggctaaaagc cgcatgtttg ccggatgcaa
     1441 cggtacggct ttacagccgt gccccttcaa aacccgatgg ccgggatgat tggaatcgga
     1501 tgttcgagat ggtttcaacc cgggaaaggc ggaattggct aatctttgca tcgctgggat
     1561 tcttcctccc ttcggtcggc agaatgacgg aaatatatcg gtcgccggaa ggacgggagt
     1621 atatagttgg tcggcagaat gagggactat aagtccagaa tgacggactg tacatatacg
     1681 cgggggaaat acagtagccg caaattttat gtaaattcgt gaaaatatga ggctttttac
     1741 gttctcgggc ttattttgag ggcaaaataa tacctggatt gtttacatat cccggcacgg
     1801 gtcttcggca aaaatagcct ctttctttca ggatatcagg ttgcgagggg gtgtccggac
     1861 aaaaccaggc ctatcctatc tgactgaaaa taatccgctt atcctgacct tgttactttt
     1921 gtaaggattg acagaggttt gggactgaaa tcctgaaaat tcagccccgt ttcattccca
     1981 ttcaatggtg cccggcggtt tggaagtaat gtcataaact acgcggttaa tgccgcgaat
     2041 ctcattgacg atgcggctgg agatccgctt gagcacgtca tacggcaggg gcacccagtc
     2101 ggcggtcatg ccgtcttcag aatgaacggc gcggatggcg caggtgtagg catacgtgcg
     2161 ctggtcgccc atgacaccga cgcttttgac gggaagcagt acggcgaagg actgccatat
     2221 ctgctggtag agtccggcgg ccttgatttc agacacaacg atatcatcgg cttcctgaag
     2281 cagggcaacg cgctcgggag taatttcgcc caggatgcgc acagccagcc ccggcccagg
     2341 gaagggttgg cgctggagaa tgtcgtccgg cattctgagg tcgcggccaa tgcggcggac
     2401 ttcatccttg aacaaatcac gcagcggctc gatgagcttg agcttcattg tttccggaag
     2461 gccgccgacg ttgtgatggc ttttgatggt ctgcgagggg cctttgacgg aagaggattc
     2521 aatcacgtcg ggataaagcg tgccctgcac caaccagtcc acgccgccgg tttgctgcgc
     2581 aatgcgatgg gcctcgtcat cgaagacagc gataaactcg ttgccgatga ttttgcgctt
     2641 ggcctctgga tcggtcactc cggcaagctt tgccagaaag cgtccgctgg cgtctacggc
     2701 caccagattc aggccgagct tgccgcgcag gttttcctgc actttgaaga attcgttttt
     2761 gcggagcacg ccgttgtcta cgaagacgca ggtgaggcgg tcgccaatgg cgcgatggac
     2821 aagcacagca gcgacggaag aatccacgcc gccggagagc gcgcagatgg cgtggccgtc
     2881 gccgactttc tgccgaatcg cagaaacagt ggactgaatg aagtgctccg gcgtccagtc
     2941 cggcttcgcc ttgcagatgt tgaacagaaa gttacgcagc agctctgcgc cgcgcggcgt
     3001 gtggtgcact tccggatgga actgcaccgc ccagatgttc ttttccgggt gagcaatgcc
     3061 cgccacagcg ttggatgttt ttgcgatgag atgaaagccg ggaggcaaat ccagcgcttc
     3121 gtctccgtgc gacatccaga cttgcagcga agatggaagt ccggcaaaaa gtggcgtggc
     3181 cgggtcaacg acggagactt cggcgtggcc atactcgcgc ttttccgccg aacggacttt
     3241 tccacccaag tggtgcgtga tgaattgcag tccgtagcag atgcccagaa caggcttgcc
     3301 cagcctgagc acagcggggt cagccggagg cgcatcggag tcatagacgg aactggggcc
     3361 gccggaaaga atgatgccga gcggtttgta gctctcaatc tcctcgagcc tggcagtgca
     3421 gggcaaaacg acggaaaaaa cattctgctc gcggatgcgg cgcgcgatga gttgtgtgta
     3481 ttgagaaccg aagtcgagaa tgacgatcga agacgtgtcc acagtccagt ttatctcgaa
     3541 gaaagacccg gtccacgatt cagggcgcgc gaagggccca tagtaagatg agaacgatgg
     3601 gatttactgc aatctggcag gtcaaccgca tctctgaata tcactggatc aagtttgtat
     3661 ttggcgacct gatttcagaa gaaatattcg acggcaagca tgaagtggtt acggaccact
     3721 gcttcgtcgt tgatcagtac ctgcatgcaa aacctgctgc ctattaccgc tcttttcagg
     3781 gaaaagaggc atttctcatc catctgtccg acgaaaccta tgaaggtggc tacgaacact
     3841 atcagcattt tcgcggtgta tttcgtaact attggtctgc ggtctttcat cccgaccatg
     3901 taatgacctt tcccctggga tttacacatc tgccgctgaa tcccgacaac ctgcctaaag
     3961 cgcaggagcg aaactatgtg tggagtttcc tggggcaggc caataaagcg tcccgtcctg
     4021 agatgttgca ggccttccgc cctgtcgggc cacagttcac atatattacg gacggccagg
     4081 accagcaaca ggcgccattg tcgcgtgaag aatatcagag ggtgttattg caatcggtct
     4141 ttgtcccgtg tccgatgggg aacgtcaacc tggaatgctg gcggatttat gaggcgctgg
     4201 aatgcggcgc catacccatt gtcgaacgcc gcgcgacact cgattatttt ggaaatctgc
     4261 tgggtccgaa tccattgcct gcttttcgca aatggagcga ggcccgtgat ttcgtccaga
     4321 aaatgaccac agaccccggc aggctcgaag acttccgtac ccaatgcgtt gcctggtggg
     4381 agcaatacaa gctcgacctg aaagggcggg tcagggactt cacgcaaaag ccgcaaacag
     4441 catcgccaaa gtccgtggtc tccggtgtct accgtatacc gggctggcac ggtcttgaat
     4501 tgttgcgaca tcattcgctc ggggcaatgc agaaacgtat ttcgttacag gcgcaccgtc
     4561 tgtttgtgca gcgaagactg cgcgtgactg ccggacgcgg atgaaatctg ccagaactga
     4621 atgaaggcga ccagccgcac aacctggaag aaatcagaag cagcaagata gaggagggaa
     4681 acaaccgccc accaggcgta cagctctgtt ccctgcataa ctgttgcgaa aggcaggggc
     4741 gaagctgaga agaccatcgc cagcacaatc agcgccagct tgatgattcc cattacgaga
     4801 ttcacttcca ccagcttgcc cgtcagcggg ccgagccgaa gactgcgcac aagactgctg
     4861 ctgacgctgc gattctccag caggaccagc aagggtgcga tggaaaaaac ccaactgagc
     4921 aatgcccaga gggcaaaaat tccgagcgac agagaaatga cgagcgcaaa atacagcaca
     4981 aggcccgggt ctgtgccgga aagcgagtag tttgcggccc aatggaccgc agcaaaccat
     5041 ccggtaaaag tgccggccag cgcgatgacg cgaagcagtt gcagcacaat cagcgagcca
     5101 ggccgcttcg gcagcgcagc atcatagcgg cgcagaacaa agtcccggcc caggcccgat
     5161 gcgatggccc atcccacggc cagaagcgga gcaagccaca gcaaaacatg cagcactgga
     5221 ggccaaagga cggcaaatgc gtccgcgatg atgaccgcgg cccgcatggg gtcctgaagc
     5281 gaaaactgag agatgcctgt cgcttcaagc cggctggcag ttgcagagta gacccgccac
     5341 aattcataac caagcagcgc aagcgcaggg acaccgaaga gccagcgcca cgcaacctca
     5401 agcagcagca gagagggacg tttccagcac ccgccgatgg tatggacgag cgactgagta
     5461 ccgcggacct gttgcgtggc gtccatttat ttcacaacaa tgttgaccag cttgcccgga
     5521 acgacgatga ccttgacgat ctctttcccg gcagtggccg tttgtacttt ggtgtctgat
     5581 ttgacggcct cgcgcatcga gtcctggtcg gccccggcag cgacctttac aaccgcgcgc
     5641 agcttgccgt tgacctgcac cgggatttca atttcatcct cacgggcgag tgcttcgtcg
     5701 aacttcggcc agggcgtgcg cagaatgctc tgctctttgc cgagctgctc ccagagctca
     5761 gcggcaagat acggcgcaaa gggggccagc agcaggacaa ggttttccag cagagttttt
     5821 accacgccgg ccgggacctt tccagcgctg atctcttcct cggcagcggt gagcgtgttc
     5881 accagctcca tgatggcggc gatgcaggtg ttgaagtgcc agcgtccggc gaagtcttct
     5941 gtgatcttgc cgatggtctg gtgcagcttg cgcaaaaggg cctgcgaagg cgcatcggct
     6001 gcaattgtat ctgttgcagt tacagaacgc gctgtcccgg catacctcat cacaaagcgg
     6061 tagaccttgc ccagaaagcg gctgactccg gccacaccgt cttcctgcca gtcgaggtcg
     6121 cggtccggag gcgcggcaaa cagggcgtac atgcgggttg cgtcggcgcc gtagcgggcc
     6181 accatgtcat cgggggagac tacattgccc ttggacttcg acattttcgc gccattctta
     6241 atgaccatgc cctgggtgaa gaggcgtgtg accggctcgt cgttttgaat gagaccgagg
     6301 tcgcgcatca ccttcgtcca gaagcgcgag tagatcaggt gcagaatggc gtgctcgacg
     6361 ccgccaatgt actggtcaat cggaaaccag taggctgcag tcgtgacgtc gaagggccct
     6421 ttgtcgaact tcgcgctggt gtagcggtag aagtaccagg atgagtccac aaaggtgtcc
     6481 atggtgtcgg tttcacggcg cgcggggcca ccgcacttcg gacaggtggc gttgacgaac
     6541 tccggcagct tgcccagagg cgagccgccc tgctgtgtaa tctcgacgtt ttccggcagc
     6601 aacacgggca gctgatcatc cgggacagga acgatgccgt ccttttcgca gtaaagcatc
     6661 gggatgggtg tgccccagta gcgctgacgg ctcacacccc agtccttcag gcggaaggtc
     6721 accgtcggct tcccgaagcc tttttcctgt gcataggcgg ccattttatg ctgcgcttcc
     6781 ctgcagccga gcgtgttgaa ctcgccggag ttgatgagca ggctgtcttc gctggtgaac
     6841 ggaagcatcg cttcttcttt gccgtcttcg tctggttcgc cattgcggcg cggcagaatg
     6901 acgacgcgga tttcaaggcc atacttcttt gcaaagtcgt aatcgcgctc gtcgtgcgcg
     6961 ggtacggaca tgatcgcgcc cgttccatac tcggccagaa tgtaatttgc cacccagatg
     7021 ggcagccgct cgccgttgaa gggattgatg gcatagtgtc cggtaaagac gccgtgcttt
     7081 tcaatctcgc cgatgtcacc tgattcgcgg gccttcttct gctggtctaa aagcgagctg
     7141 acctcttcgg caagtttcag gtcggtcgcg gcgaactcct tcaccagcgg atgctccggc
     7201 gccagctgga tgctggtggc accgaagatg gtatcaatgc gcgtggtgaa gacggtgatc
     7261 ttacctgctg tttttgcgtt ctcgcgcgca ggcagaatag ccgtgccctt tttgcgctgg
     7321 gactgctcgt cgtccggcgc gctggattcg tcttctaccg cgaagtcgac ctccgcgcct
     7381 tcgctgcggc caatccagtt gcgctgcatg gtgcggacct tttccggcca gccatcgagc
     7441 ttttctaaat catccagcag ctcttgtgca tagttggtaa tgcgcaggaa ccactgctca
     7501 aggtcgcgct gttcaacgat tgtgtcctca tgccgccagc agcgcccgtt gatgacctgt
     7561 tcattcgcca gcaccgttgc gcattccggg caccagttca ccttgctctt tttgcgataa
     7621 gcaagaccct tctcaaacat gcgaatgaag aaccactggt tccaacggta gtagtcaggc
     7681 aggcaggtgg tgacttcatt gcgccagtcg tagctgaaac cgatgcgctg catctgccgc
     7741 ttcatggcag cgatgttgtg cagcgtccat tcgcgcggag gcgtattgtt cttaagcgca
     7801 gcattttccg caggcaggcc gaaggcgtcc caacccatgg gatgcagcac gttatgacct
     7861 tgcatccaca tataacgggc gagcgcgtcg ccaatggagt agttgcgcac gtggcccatg
     7921 tggagctggc cggaaggata cggcagcatc tcgagcacgt agtactttgg cttaccactg
     7981 gaagcaggtt cgacggcata caggccgggc tgcgcagccc agcgctcctg ccagcgcggt
     8041 tcaatttctg ccggattgta gcggtactct tttacggccg gtgcagccgc agtctcaggc
     8101 ttgcttgacg acatgtattt attttaccgg gactgcgctg gccccttgct gaacgcgatg
     8161 ttttcaggct gctttcatcg gctggatctt cggacgcttc cgccgccttg cctgggccag
     8221 ttcatattgc ttctgtaagt ccatccagaa ctcactcgga ttgccgaaga aggcctccaa
     8281 gcgcagagcc atatcggcag agatgccagc tcttccgttg atgattttag acaatgtagg
     8341 ccgcgctatc ttaagacggg ccgccgcctc tgagatatcc atgctgccta aagacaactt
     8401 cagaatcctg ccgggatgca ccggattcag catcttcata catgcctctt ttcagtgata
     8461 atcctgataa tccacaaggg ccacatcctc ttctttaaga gtaaatgtga tcctccagtt
     8521 tccacctacc gacaggccgt agtggccctc caacttaccg tgcaagggat gcaagttcca
     8581 tgctacggca agatttcctg gaacatcaga cacgcgctta ataacattca gcaccgtgag
     8641 ccgttcccgc agcttctcgg catggccagg atgaatgcct ctgcggctct ctgtttcaaa
     8701 gaatgccttc aggcctttat gccggaagct gataatcatt ctgttatctg taacgtaaca
     8761 gattacgaat gtcaactatg actaacgctg gcggccagtg aacgcagctt cgccacattg
     8821 cgctcgtcat ctcccggctg gtcgagcggc gtctcagcga taaaggcgca atgggcaaag
     8881 cgcgtgtcct gcaaaaggcg ccggaaggcc gctgcgccta tcgttccttc gccgatatgt
     8941 tcgtggcggt cgagctttga tccgcgcgcg gccctggcat cattcatgtg ccagacgagg
     9001 acatggtcga ggccaattgt tgcctcaatc tgccggacag tctcctggta accttccgga
     9061 gtgacgatgt catatccggc aacgtgggta tggcatgtgt caagacacgc gcccaccggg
     9121 gccacgttgc gcaggcggtg catcagcacg gccacctgct caaagctgcc gcccagcgaa
     9181 aactcggccc cggcagtgtt ctcaatcaga atctgaaaat ggcgtgcctg ccaatccagt
     9241 ccttcaaggg ctttctgaat ggattcgacg gccagtcgca gaccttcttc gcgcgtcaac
     9301 ccgcgccagg aaccgggatg caacaccaga tactctgccc cgagcgccag cgcacgctcg
     9361 acttcgccgt gaaatgcggc gatggacttg gcgcgcactt cctcggactg gctgcacagg
     9421 ttcaccagat agctggtgtg aatgacgagc ggccagcagc catatttctg gcgcagttcg
     9481 cgcatccgct cgcagtgcgc aggctcaatg cgagccggac gccacatgcg cgggctggag
     9541 gaaaaaatct ggacggtgtt cgcgcccatg gcctgcgcgc gctcggcggc catgtacacg
     9601 cctcctgaag tggaaaggtg tataccaatg cgcaagatat tctttttcat ctcccaagga
     9661 aagaaaagta gtagactata cagctataca agaaagctgt tcttatgcga aagatcatta
     9721 ctgccgaaaa actgttctcg ccggaagata cggtcctcca ccccattgtg gttgtggaag
     9781 acagaaccat caccagcatt gtatcccgtg aggaaggtct cttccctgaa ggcgaacggt
     9841 atgatttccc cggggccata ctggtccccg catacttcga tgtgcatttc cacggcagcg
     9901 cgggaagcga tgtaatggaa gccaccgaag gtgcgctgaa tacaatcggc agctttcttg
     9961 cgcgtcatgg ggttggctcc tactttgcaa caacaatatc agcacccgtg gacgcgaccc
    10021 tgaaatcgct cagcggactg gcaaagctta tgggcaaaga cctgaaaggc gcaaggccac
    10081 tcggcatcca tattgaaggc ccctttctct cccatgccaa gaaaggcgca catgctgagc
    10141 gcgatttgca gcagccatca gctggcctgt tcaaccgcat gtgggaggcg gctgaaggcc
    10201 acatccgcct gatgaccatc gcgcctgagc ttcccggcgc gattgaggtc atcgaacgcg
    10261 ccgtttcgct gggcgtgcgc gtcagtctcg gacacagcaa tgcatcgagc gaagacgctc
    10321 tgcgcggcgt ccaggccggg gccacgtcag ccacgcacac cttcaatgcc atgcgccgct
    10381 ttgaccaccg cgacccgggc attctgggcg ttgtccttga ccgcaatgac ctgtttgccg
    10441 aaatcatctg cgacggactg catgtccatc ccacagttgt gcgcatgttc tggaaggaaa
    10501 agggcgcagc ccgcgccatc ctgattaccg atgctatgag cgccacggga atgcccgatg
    10561 gcaactacaa gctgggcgag ttagatgttc gtgtgaaaaa cggcaagtgc atcattggcg
    10621 aagacacgct cgccggcagc acgctcactc tggaccgcgg cgttcgcaac ttcgccgagt
    10681 ttaccggagt ggacattgcc accgccgcaa cactggccac gcgaaaccca gcccgcatgg
    10741 caggactgga accacaggcc ggcacacttg cggcgggacg tccggctgac atcaccgtgc
    10801 tctcaccaaa aggagaggtg attgagacca ttcttcgcgg agagagagga tgacaagaac
    10861 ttctgactca ccccgctatc catgcaagct cttctctaat agtttcacaa tctctgcatg
    10921 tccacgctcc cgggcataat cgagggccgt cttgccctgg tcgcacttag catcgagacg
    10981 tgctccacat tcgactaaaa cctgtaccat ctcttttttg ccggcagcgg cggcctggat
    11041 gagaggcgta tatcctccat gctgggtcgc gttgacatcg gccccggcct ccagcagagc
    11101 tcgcactgca tccacggaat tgttgagcgc cacacaggca tggagaggct ggttgcgcag
    11161 cgggttgtgt gagacctggt gcgcatgcgc tccatgctcg acgagcaatg tcacagcctc
    11221 agggcctgca aaggcagcgg ccagatgcaa cggcgtccac ccgtcaggag aaacggcgcg
    11281 cacctgcatt gcgtctccgg caatcagagc ttgcagccgg gcgcaatcgc ccaccgctgc
    11341 tgcctcaaag atatccagtt cagtacagtg ggtgaggaga aagtcccgga ccaccgactg
    11401 atgtgtgtag acggccagca tgagcgctga gactccctgc gcatcccgtg aatgcgcc
//
LOCUS       DASRBV010000350         5377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_331128, whole genome shotgun sequence.
ACCESSION   DASRBV010000350 DASRBV010000000
VERSION     DASRBV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331128"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3608
                     /locus_tag="VFA02_13080"
     CDS             <1..3608
                     /locus_tag="VFA02_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZU10829.1"
                     /translation="DGPGMVIQSSDQSPLGATGNSATSVTWNPVRKRFYAAVRYHGYY
                     ESTDGATWTRLQNQPGTGLYQAQCPANPNSTGSEQCPIFRGTVTAQPVTGDLFAITVD
                     NNNVDQGLWRDACNANSGACASSTVTFSQQIGDAAIESSGKIPQGDYNLSLAAVPALS
                     DTLLFVGAKDLYKCSLLNNCIWRNTTNTDGCASAQVAPSQHAIEATFGGNGLMYFGTD
                     GGLWRTTDAVNQQQSVCSSDDAFHFQNLNSGLGPISEVGTFSVDPQNPDVIMAALGGF
                     GTAALSGSGTSWTLAVPGEGDNNQIDPQNSQNWYATSAPAGSSPFIESCSSGSACATS
                     GQWNAAIGSGQIGDDLTGPVVPWILDPQNTANIIIGTCRVWRGLASGQSWTQGNLLSN
                     PLDNDQQNSYCNGNALIRSLAASGGSGNTEIIYAGMAGWADGGASVAGHLYAATVPAS
                     GGLVTWNDLTQSPIINGQGNTFNAGGFDISSIYVDPHDASGNTVYVTRQGFITPGVYG
                     ALVYRSVDGGASWISLATNLPDAPANSILVDPNDANTVYVATDTGVYATRNIIACTNT
                     SNSCWTPLGSGLPLSPVTQLRYVSSGSTQVLLASTYGRGIWQIPLLTSGTQPTTATLT
                     PSALTFTGQTQNTVSSPQTITVTNTGPISLSITSISANQNFLENDNCSQPVPSGSTCN
                     IQVSFAPQKVGAAQGTLTVFGNLPGGQITAMLSGTGLAPGNLVVVPGSLNFGDAVVGT
                     TTSPKDITISNTGGVSVNLQQPAMTGDFQMIANTCGTSLAANTGCTVAIAFSPAASGA
                     RNGTFSIGNGSSTQTVLLTGNGQTPATAQLSATSLNFSTPVTVGTVSSPQTTTLTNTG
                     DAALAQIEVTPGGDFLVQNNCPALLPGHTSCALVVTFAPKQAGAETGNLTISTALGVQ
                     MVALSGTGLAPAGVSLLPASIDFGGQGVKITSGKHSVTLTNNGGVPLTGLSFAVTGTD
                     FSIADSTCQSGSTLAPGSACMFDLTFTPMQTQSRNGTLMVNATQLSAPYTVPLSGTGE
                     DFSISVVGQTSQVIVNGQSAAYQVQITSVDGSSGPVQVSCSGLPQNATCTSGLAAGAT
                     LTGSASMTATVTIATGVSTSAAIRWPQTMAMAFAFLPCIFISRKRKYLLRLWTMCIVS
                     LVLLVSTACGTSASGGSGSAPGTPTGSTPSNTYTININATLDGLTKSAAVQLTVQ"
     gene            3694..4443
                     /locus_tag="VFA02_13085"
     CDS             3694..4443
                     /locus_tag="VFA02_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10830.1"
                     /translation="MKLSACAWIFSAMAMCTVYAQQQPAPDTTAGQQQTQTRTIQMAP
                     ARATLNHGLDAKKAKQGDAVTAKLQDDVKIADAPELPKNTVLMGHVDQVQPSEHKSDS
                     VIQVTFDKAQLKGGQQVPIKATVMQIALPVNAMMMGQDAGGGSSGAPMASPSMPSSAP
                     SSGGSAGSGSGTQTSSAPPPTPSPSMGSAPVPDQMQQSQQQGVPGVELKSDIHQQDSA
                     TFTSKGKNVHLPDGTQMQIAVAVVPPNTQIK"
BASE COUNT         1246 a         1490 c         1476 g         1165 t
ORIGIN      
        1 aggatgggcc gggaatggtg atccagtcct cggaccagtc accgctgggt gctacaggaa
       61 actcggcaac ctcggttacc tggaaccccg tgcgcaagcg tttctatgcg gcagtgcgtt
      121 atcacggcta ttacgaatct actgacggcg ccacctggac ccgccttcag aaccagcccg
      181 gcactgggct ttatcaggcg cagtgcccgg caaaccccaa ctcgaccggc tcagaacagt
      241 gccccatctt tcgcggaacg gtgactgcgc agcctgtgac cggagacctc tttgctatca
      301 ccgtggacaa caacaatgta gaccagggat tgtggcgaga tgcgtgtaat gcaaacagcg
      361 gtgcctgtgc ttcatctaca gttacttttt cgcaacaaat cggagatgcg gcgattgaat
      421 cgagcggcaa aattccgcag ggcgattaca atctttcgct cgccgctgtg cctgcgctct
      481 cggacacgct gctctttgtc ggcgcaaagg acctttacaa atgcagcttg ttgaacaact
      541 gtatatggcg caacactacc aatactgacg gctgcgcatc ggcgcaggtc gcgccttccc
      601 aacacgccat tgaggccacc ttcggcggga atggtctgat gtatttcggc accgatggag
      661 gactttggcg caccacggac gccgtcaacc agcagcagtc tgtttgctct tccgatgatg
      721 ctttccattt tcaaaacctg aacagcgggc tgggccctat cagcgaggta gggacgtttt
      781 ctgtagaccc gcaaaatcct gacgtgataa tggcagcgtt gggcgggttc ggaacagcgg
      841 ccctttccgg cagcggaaca tcatggacgc ttgcggtgcc cggtgaaggc gacaataacc
      901 agattgatcc gcagaattcg caaaactggt atgccacgtc agcacccgcg ggctccagcc
      961 ctttcatcga atcgtgctct tcgggaagcg cgtgcgctac gtctggccag tggaatgccg
     1021 cgataggcag tggacaaatc ggagatgatc ttacagggcc tgttgtgccg tggatccttg
     1081 acccgcaaaa tacggccaat atcatcattg gcacatgccg cgtatggcgc ggtcttgcaa
     1141 gtggacagag ctggacacag ggcaatctgc tcagtaatcc gctggacaac gatcagcaga
     1201 acagctactg caacggcaac gcgctgattc gctcgctggc ggcatccggg ggcagcggca
     1261 acacggaaat catctatgca ggcatggcgg gatgggccga cggaggcgcg tcggttgcag
     1321 gccatctcta tgcagccacc gtgcccgctt ccggcggcct ggtaacgtgg aatgacctta
     1381 cgcagtcgcc gatcatcaat ggtcagggaa atacgttcaa tgcgggtggc tttgatattt
     1441 ccagcattta cgtagaccca cacgacgcat cgggaaacac cgtgtatgtt acgcggcagg
     1501 gattcattac gccgggagtt tatggagcat tggtatatcg cagcgtggat ggaggcgcaa
     1561 gctggatttc ccttgcgacc aatctgcctg atgccccggc caacagtatt ctggtggacc
     1621 cgaacgatgc caacacggtc tatgtggcga cggatacagg cgtgtacgca acgcgcaata
     1681 tcattgcctg cacaaacaca agcaattcgt gctggacgcc tcttggctca gggctgccct
     1741 tgtctccggt tacgcagctc cgctatgttt cttcaggaag cacacaggtg cttctggctt
     1801 caacctatgg gcggggaatc tggcagattc cgctgctgac gtcaggcaca cagcccacga
     1861 cagccacgct taccccgtca gcgctcacat ttacaggcca gacacagaac actgtcagca
     1921 gcccgcaaac gattacagtc acaaacaccg ggcccatttc gctgagcatc accagcatca
     1981 gtgcgaacca gaattttctg gagaatgaca actgttcgca gcccgttcct tccggttcca
     2041 cgtgcaatat tcaggtcagt tttgctccgc agaaggttgg ggcggcccag ggcacgctta
     2101 cagttttcgg caaccttccg ggagggcaaa tcacggccat gctttcgggg acagggctgg
     2161 ccccgggaaa cctggtggta gtgcccggaa gcctgaattt cggagatgcc gttgtgggca
     2221 caaccacgtc tccgaaagac atcaccattt ccaatacggg aggtgtaagc gtcaatctgc
     2281 agcagcccgc aatgaccgga gactttcaga tgattgccaa cacctgcggc acctcgcttg
     2341 cggcgaatac aggctgcacg gttgcgattg cattttctcc ggctgcatct ggcgcgcgga
     2401 acggaacgtt cagcatcggc aatggatcca gcacgcagac ggtgctgctg actggcaatg
     2461 ggcagacacc ggccacagcg cagctttctg caacaagcct gaatttctca acgcccgtga
     2521 ccgtgggcac agtaagcagt ccccagacca ccacgctgac aaacacaggc gatgcggccc
     2581 tggcgcagat cgaagtaacg ccgggcggag attttcttgt tcagaacaac tgccctgcgc
     2641 tgcttccagg gcatacatcc tgtgcgcttg tcgtgacatt tgcgccaaag caggcaggtg
     2701 cggaaacagg aaacctgacc ataagcacag cgctcggcgt gcagatggtc gcgctttcag
     2761 gaacaggcct tgctccagcc ggagtgtcgc tgctgcctgc atccattgac tttggcggac
     2821 agggcgtcaa gattacgagc ggcaagcatt cggtgacgct cacaaataat ggcggagtgc
     2881 ctctgactgg attgagcttt gctgtgacag gcaccgattt ctccattgcc gattcgacat
     2941 gccagtcagg aagtacattg gcaccaggca gcgcctgcat gtttgatctc acatttacgc
     3001 caatgcagac acagtcgcgc aacggaactt tgatggtgaa cgccacgcag ctgtctgctc
     3061 catacactgt gccgttgtct ggaacaggcg aagacttcag tatctctgtt gtgggacaaa
     3121 cgtcacaggt aattgtgaat ggccaatcgg cggcctacca ggtgcagatt acctccgtag
     3181 atggctcaag cgggcctgtg caggtgagtt gctccggtct tccgcagaat gcaacctgca
     3241 cgtcggggct tgctgccgga gcaacgctta cgggaagcgc ctccatgacg gcgaccgtca
     3301 cgatcgctac tggcgtgagc accagcgcag ccattcgctg gccgcagacc atggccatgg
     3361 catttgcatt tctgccctgt atttttattt cacggaaacg aaaatatctg ctgcgtttgt
     3421 ggacgatgtg catcgtttct ctggttcttc tggtttccac agcctgcggt acaagcgcca
     3481 gcggagggtc ggggtctgct ccggggacac caacaggctc cacgccttca aacacttaca
     3541 ccatcaatat caatgccact ctggatggat tgacgaaaag cgcggcggtc caactgacag
     3601 tgcaataggt agaagaaaac tcaaaagtac ccgggacaag gcaacagaaa accgcgcctc
     3661 ctcttcccaa cttaaggaaa aggagaattt gctatgaagc tgagtgcatg tgcatggatt
     3721 ttcagcgcaa tggccatgtg cactgtgtat gcgcagcagc agccggcccc ggacacaacg
     3781 gcgggacagc agcagaccca gacgagaacc atccagatgg cccctgcgcg cgccacgctg
     3841 aatcatggac tggatgccaa aaaagcaaag cagggcgatg ccgtgacggc caaactgcag
     3901 gacgatgtaa agattgctga cgctccggaa ttaccaaaaa acactgttct gatggggcat
     3961 gtagaccagg tgcagccgtc tgaacacaag agcgattccg tgattcaggt cacgttcgac
     4021 aaagcgcagt tgaaaggcgg gcagcaggtc ccgattaagg cgaccgtcat gcagattgct
     4081 cttccggtga atgccatgat gatgggacag gatgcgggcg gtggaagctc tggagcgcct
     4141 atggcgagtc catctatgcc atcctctgcg ccttcttcgg gtggatcggc tggcagcggc
     4201 agcgggacgc agacttccag cgcgccccct ccaacgccgt cacccagcat gggcagcgct
     4261 cctgtgccgg accagatgca gcagtcccag cagcagggtg ttccaggcgt agagctgaag
     4321 agcgacattc accagcagga ttcggcaacc ttcacttcca agggcaagaa cgtgcatctg
     4381 cctgacggca cgcagatgca gatcgctgtg gcggtggttc ctccgaatac gcagatcaag
     4441 taacaccaca aaccacaaag agaagtgcct taacggttcc gattcgcaat tcggggccgt
     4501 taaggtattt cctgcgctat aggattttca cagatcgtct gaaccatgcc ctggggaact
     4561 acagtcgccg cgaccaacag ggagccggcg aacactgctc aatacaaatc gagtgtactc
     4621 atagcatttg aacgaccccc taccccccgt gttttgtacg ggaatccttt agtttcaata
     4681 agttacgagt gatgacttgt acgggaatcc tttgttttca ataagttagc aaagaagcgg
     4741 ccagcagtca gcgcctgctt cgcaggctgt cagccaagag acttccaaac ttctcagctt
     4801 cccatagata atctaggtat atataaaagt cagtgtgaat ttttttgtaa attcttgaaa
     4861 gtaatgtagt tatttaattt tacgctttca tttttcgaaa atttttatac ctagattgtc
     4921 tacttacgcc cggattccgg taccctcggc ccctgattat ttttgccctt tctttcagga
     4981 tatcaagtgg aagaggagaa tccggacaaa ggccggcatg gcttatctta ttgaaaaaaa
     5041 atggattcga tggatttatt actttcgtaa gggttgacaa ttcccgcggc ctcttatttg
     5101 acgggtaact accatgtttt caggacttta gcacttttgg tatataagag tccgtcaatt
     5161 ttgacgaccg aaggttggag aaatctcagc gattccggtt ccagccagtt ccgcgccgcc
     5221 cggaatgatg atcataagtg aaacgaaaaa tgcttcagtg caccttggct tcggccagcg
     5281 tggcggggat gttcagataa aagtggccgc cccatacatc gtctttgtca ttttcagctt
     5341 tgactacggg cttgccgccg gtcatatgct ggtcctc
//
LOCUS       DASRBV010000351         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_431958, whole genome shotgun sequence.
ACCESSION   DASRBV010000351 DASRBV010000000
VERSION     DASRBV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431958"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFA02_13090"
     CDS             <1..214
                     /locus_tag="VFA02_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10831.1"
                     /translation="GWERGATRVQGQGNTWVEEPTDRVLSFVYQELETDSSPEVKKWA
                     EETWRSKKAGNTEELLQKYGPAPEKL"
     gene            complement(264..2141)
                     /locus_tag="VFA02_13095"
     CDS             complement(264..2141)
                     /locus_tag="VFA02_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HZU10832.1"
                     /translation="MRRFSFCGCLIALLLASIPVFAADPITITADLSEAPRKLWHAEI
                     TIPVQPGPLTLTAAKWIPGTHMPSGPIDNITGIVFRAGGKTLPWRRDDIDLYAFHVTV
                     PEGVSTLNVHVDAIVTARVSDKLAMLEWEKVLIYPASTPVRNIEIQPSIKVPEGWGVG
                     TALTPIGTEGNTTHFAPVSLEMLEDSPILTGKYFKEIALAPDVTPKHYLDVAGDAPED
                     INLRPQFLTALNNLVHEASAMYSSHHYNSYHFLLTLSDYAGGEGLEHHQSSDNGVDEK
                     GFADDQHELIEADLLCHEFTHSWNGKYRRPAGLATPDYATPMKGELLWVYEGMTQYLG
                     NILAARSGFRTPEQYREALAFSAAQLDNRPGRTWRNTEDTAIAASILRGGNPAWSNLR
                     RGQDYYQEGELLWLDVDTTIRQLTHNKKSLHDFLVLFVGKGGNTGPMVVPYTFDDLVT
                     LLNQVVPNDWAGFLHERITTLSPHADLAGIEHGGYKLVYTDKPTSYERALLARRGGYD
                     GWFSAGLRLGRDGDIMDVRVNSAADQAKLFPGERIIAVNGRVFSPEVFGDVLKETKGS
                     SAPVHLVVQSDTYVYSVDLDYHDGEKYPRLERVEGIPALLDDIIKPMTNVQPIPTEKK
                     S"
     gene            2140..>2496
                     /locus_tag="VFA02_13100"
     CDS             2140..>2496
                     /locus_tag="VFA02_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10833.1"
                     /translation="MKMCPFMMRVNHLWYGRKLYCIYPTSTDISVHLESILFKTRETV
                     AERKQRVPVRELEKLAAEHTPRRFANVLSRRAQDGPAIIAELKKASPSKGLIRSEFDV
                     RALASELEQAGAAALSV"
BASE COUNT          562 a          632 c          746 g          556 t
ORIGIN      
        1 tggctgggaa cgtggcgcaa cacgggtgca gggacagggc aatacctggg ttgaagaacc
       61 aaccgaccgc gtattgagct ttgtttacca ggagttggaa accgatagct cgccggaagt
      121 aaagaagtgg gccgaagaaa catggcggtc gaagaaggcc ggcaacacgg aagagctgct
      181 tcagaaatat ggtcccgctc ctgagaaact gtagagccgt gatgtcccat ccttcacatt
      241 gcgaaggata ggacatcacg catttacgac ttcttttctg ttggaatggg ctgcacgttt
      301 gtcatcggtt tgatgatgtc atccagcaac gctggaattc cttccacccg ttcaagccga
      361 ggatatttct cgccatcgtg ataatcgaga tctacggaat aaacatacgt atcagattgc
      421 acgacaaggt ggaccggcgc gctgcttccc tttgtttctt tgagcacgtc accgaaaact
      481 tctggagaga agacccgtcc gttgacagcg ataattcttt cgccggggaa gagttttgcc
      541 tgatcggcag ccgagttcac gcggacgtcc ataatgtcgc cgtcgcggcc gagacgcagt
      601 cccgcggaaa accacccgtc atagccgcct ctgcgcgcga ggagggcgcg ctcatagctg
      661 gttggcttgt ctgtgtaaac gagtttgtag cccccgtgct cgattcccgc aagatcggca
      721 tgagggctga gcgtagtgat gcgctcatgc agaaaaccgg cccagtcatt aggaacgacc
      781 tgattcagca gagtgacaag atcatcaaag gtgtagggca caaccatggg accggtattg
      841 ccgcctttcc caacgaaaag cacaagaaaa tcatgcagcg attttttgtt gtgcgtgagc
      901 tggcggatgg tggtgtccac gtcgagccat agcagttcgc cttcctggta gtagtcctgc
      961 ccgcggcgca agttcgacca ggcgggattg cctccgcgca gaatggaagc ggcgatggcc
     1021 gtatcttcgg tattgcgcca ggtgcgtccc ggacggttat caagctgcgc agcggaaaag
     1081 gcaagcgcct cacgatattg ctccggcgtg cggaaaccgg aacgcgcagc cagaatgttt
     1141 ccgagatact gcgtcatgcc ttcatagacc cacagcaatt cgcccttcat cggagtggcg
     1201 tagtcgggtg ttgccaatcc tgcggggcga cggtatttgc cattccagga atgcgtgaat
     1261 tcatggcaga ggaggtcggc ttcaatcaat tcgtgctgat cgtcggcgaa acctttttcg
     1321 tccacgccgt tgtctgaaga ctgatggtgc tccagccctt cgccgcctgc atagtcagag
     1381 agcgtaagca ggaagtgata actgttgtag tggtgcgagc tgtacatggc gctggcttca
     1441 tgcaccagat tgttgagtgc ggtaagaaac tgcggacgca ggttgatgtc ctccggcgca
     1501 tctccggcaa cgtccagata atgcttcggc gtcacatcgg gcgccagagc aatttccttg
     1561 aaatatttgc cggtgagaat cggcgagtct tcaagcatct ccaggcttac aggtgcgaaa
     1621 tgggtcgtgt tgccctcggt gccgatgggt gtgagggcag tgcctacgcc ccatccttcg
     1681 ggaactttga tagaaggctg gatttcaatg ttgcggactg gggtgctggc gggataaatc
     1741 agcacttttt cccattcgag catggccagc ttgtcagaaa cgcgcgcggt cacgatggcg
     1801 tctacatgca cgttgagcgt ggaaacgcct tcgggcactg taacgtgaaa tgcgtacagg
     1861 tctatatcat cgcgccgcca gggcagcgtt tttccgccgg cgcggaagac aattccagtg
     1921 atgttgtcta tcgggccgga aggcatgtgc gtgccgggta tccacttggc cgcggtgagc
     1981 gtgagcggtc ccggttgtac tgggatcgta atttcagcat gccagagctt gcgcggagct
     2041 tcagagagat cggcagtgat ggtaataggg tctgcggcaa agaccggtat ggatgccagt
     2101 agaagggcga taaggcaacc acaaaaagag aatcggcgca tgaagatgtg tcctttcatg
     2161 atgagggtca accacctatg gtacgggaga aagctatact gtatctatcc cacttcaacg
     2221 gacatctcag tgcatctgga aagcattctt ttcaagaccc gcgaaactgt tgctgaacgc
     2281 aaacagcgcg ttccggtgcg cgaactcgaa aagcttgcgg ctgagcacac tcccagaagg
     2341 tttgccaatg tcctcagcag aagggcgcag gatggaccag cgatcattgc tgagttgaag
     2401 aaagcatcgc catcgaaagg cctgattcgg tctgagtttg atgttcgtgc ccttgcttct
     2461 gagttagagc aagctggcgc agcggcgctt tctgtg
//
LOCUS       DASRBV010000352         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_593848, whole genome shotgun sequence.
ACCESSION   DASRBV010000352 DASRBV010000000
VERSION     DASRBV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593848"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFA02_13105"
     CDS             <1..747
                     /locus_tag="VFA02_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HZU10834.1"
                     /translation="QPWIKPEQPGEIMRDVFLTRGPDHKFHMVWTWGWHGNSLGYAES
                     DDLIHWSAQKEVPIMQDYPDTRNVWAPEIYWDAKQQKWLVIWSSSMNSSDAGNRIYAS
                     FTRDFTTFSRPQIFFDPGYVVIDATIFHWKKTYYLVFKDQTPDPLRYQVRFATGPTLE
                     GPWNEIHPPLTESWSEGPSVVHVGDKFIVYYDHYRAPARYEAVQSTDWKHWESINDRI
                     SLPEHCKHGSFMEITPEEATRLEAWGSTEQ"
     gene            complement(800..2311)
                     /locus_tag="VFA02_13110"
     CDS             complement(800..2311)
                     /locus_tag="VFA02_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HZU10835.1"
                     /translation="MNRREVMKLSAGAALGMRSAMGDAFSAETPHVEEVEQWGTFETA
                     FHGPSAGNPFTDTKLTARFTLQHRTVEVAGFYDGEGTYRVRFMPDMQGRWSYETESNA
                     VKIHGKRGSFQCVAPSPSNHGPVQIAHQYHFQYADGTPYSPFGTTCYSLAFLGEELEN
                     ETLQTLKSAPFNKVRMCLLPKPLGSTKLFAYPFERDAAGKNDYMRPVPEYFRHIERRI
                     LDLQKLGIEADVILFHPYDAWGYKAMPAEADENYLRYAIARLSAYRNVWWSLANEYDL
                     VKTKTPSDWDRFFRIVVASDPYSHLRSIHYSGVLYDYSKPWVTHASLQNYQFEKAAEW
                     RAAWRKPIIYDEIQYEGNIARRWGNLSAQEMTRRFWLAIVAGAYATHGETYISTDGKP
                     VWSDGGTLHGTSPARIGFLRKLVEESTRVGLNEFEGPYYLSAGTANELYLWFFDYHQP
                     AEYVFPLPEKVQFQADIIDPWEMTIRPVDGRFSGKSKLQLPGKPYQAVRFRKA"
     gene            complement(2308..>2973)
                     /locus_tag="VFA02_13115"
     CDS             complement(2308..>2973)
                     /locus_tag="VFA02_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HZU10836.1"
                     /translation="ADSDSISNLTIANDAGSGKGQAVALAVTGDRAVFRNLRILGAQD
                     TLFAASRYCYGDYGPCVPARQYFRDCYIAGNVDFIFGDAIAVFDHCELYGIPGHVMYT
                     AQSRHTAEQQSAYVFDRCRLTADAGAQTVSLGRPWRPYATVVYLHTQMDAPVIPAGWT
                     EWPRFGVPSLPTAYYAEFDSTGPGANPKAREPYSHQLTATEAAQWSPRKVLAGEDGWD
                     PTK"
BASE COUNT          646 a          923 c          797 g          607 t
ORIGIN      
        1 cagccctgga tcaagccgga acaacccgga gagatcatgc gcgatgtttt cctcacccgc
       61 ggtcctgacc acaaattcca tatggtctgg acctggggat ggcacggcaa cagcctcggc
      121 tatgccgaat cggacgatct gatccactgg tccgcacaaa aagaagtccc tatcatgcag
      181 gactaccccg acacgcgcaa cgtatgggcg ccggaaatct actgggacgc gaagcagcag
      241 aagtggctcg tcatctggtc aagctcgatg aatagctccg acgcaggcaa tcgcatctat
      301 gcatccttta cgcgcgactt caccacattt tccagaccac aaatcttctt tgaccccggc
      361 tacgtcgtga ttgacgcaac catcttccac tggaagaaaa cgtactacct cgtcttcaag
      421 gaccagactc ccgacccact gcgctatcag gtgcgcttcg ccaccggacc aacgctcgaa
      481 ggcccctgga acgagatcca tccgccgctg accgaatcct ggtcggaagg cccgtccgtc
      541 gtgcatgtgg gcgacaagtt catcgtgtac tacgaccact atcgcgcccc cgcgcgctac
      601 gaggccgtcc agagcaccga ctggaaacac tgggagtcca tcaacgaccg catcagcctc
      661 cccgaacact gcaaacacgg cagctttatg gaaattactc ctgaagaagc aacgcgcctg
      721 gaagcatggg gttcgacgga acagtaaggg atatagcctg ctctcataag acacgagcag
      781 caatgtgcca gaacccaaat catgccttcc ggaaccgcac tgcctgatac ggctttccgg
      841 ggagctgcaa tttcgacttc ccggaaaatc ttccgtccac gggcctgatg gtcatctccc
      901 acgggtcaat gatatcggcc tgaaactgca ccttctccgg cagcggaaag acatattccg
      961 cgggctggtg atagtcaaag aaccacaaat acagctcatt cgccgttccc gcagaaagat
     1021 aatacgggcc ttcaaattca ttaagcccga cacgcgtgct ttcctcgacg agcttacgca
     1081 gaaagccgat gcgcgccgga ctcgttccat gcagcgtgcc accgtccgac caaaccggtt
     1141 tgccgtctgt gctgatgtag gtttctccgt gcgttgcata cgcaccagct acgatggcca
     1201 gccagaagcg ccgcgtcatc tcctgcgcag agagattgcc ccagcgccgc gcaatgttcc
     1261 cctcatactg gatttcgtca tagataatcg gcttacgcca ggctgcgcgc cactcggccg
     1321 ctttctcaaa ctggtagttt tgcaggctcg catgcgtcac ccagggtttg gagtagtcgt
     1381 agagcactcc gctgtaatgg atggaacgta gatgcgaata agggtcagag gcaacgacaa
     1441 tgcggaagaa gcggtcccag tctgacgggg tcttcgtctt gaccaggtcg tactcgttcg
     1501 ccagcgacca ccacacattt ctataggcag aaagccgcgc aatcgcataa cgcagatagt
     1561 tttcgtccgc ctcggcgggc atggccttgt atccccatgc gtcatagggg tgaaacagaa
     1621 tcacatcagc ctcgatgccc agcttttgca ggtccagaat gcgccgttca atgtgacgga
     1681 agtattccgg caccggacgc atgtagtcgt tttttcccgc ggcgtcgcgc tcaaagggat
     1741 acgcaaacag cttcgtgctc cccaaaggct tgggcaacag acacatgcgg accttattga
     1801 aaggcgctga cttcagcgtt tgcagcgtct cgttttccag ttcctctccc agaaacgcca
     1861 gcgaatagca ggtggtcccg aagggagaat aaggagtgcc gtccgcatac tggaagtgat
     1921 actggtgcgc aatctgcacc ggcccatgat tgctcggcga aggcgcaacg cactggaaac
     1981 ttccgcgctt gccatgaatt ttcaccgcat tgctttcggt ctcatagctc cacctgccct
     2041 gcatatcagg catgaagcgg acgcgatatg ttccctcgcc gtcgtagaag cccgcaactt
     2101 caaccgtccg atgctggagc gtgaagcgcg ctgtcagttt cgtgtctgta aaagggttcc
     2161 cggcagaagg cccatgaaat gccgtctcaa atgtgcccca ctgctccact tcctctacgt
     2221 gcggcgtctc tgctgaaaaa gcatccccca tcgccgagcg cattcccagc gcagcacctg
     2281 cgcttaactt catcacttca cgtctgttca ttttgttggg tcccatccgt cctcacctgc
     2341 cagcactttg cgtggcgacc actgcgccgc ttctgtagct gtaagttggt gcgaatatgg
     2401 ttcgcgcgct tttggatttg cgcccggccc ggtcgagtcg aactccgcat aataggctgt
     2461 cggcaggctg ggcacgccaa agcgcggcca ctctgtccat cctgccggga tgaccggagc
     2521 gtccatctgc gtgtgcagat ataccacggt cgcgtaaggc ctccacggac ggccaagtga
     2581 gacagtttgc gcgccagcat ccgcggtcag acggcagcgg tcgaagacat acgcgctctg
     2641 ctgctccgca gtgtgcctgc tctgtgccgt gtacatcacg tgcccgggaa ttccgtacag
     2701 ctcgcagtgg tcgaacacag cgatcgcatc gccaaagatg aagtccacgt tgcccgctat
     2761 atagcaatcg cgaaagtact gccgcgccgg aacgcatggc ccgtaatcgc cgtagcaata
     2821 gcggctggcg gcaaagagcg tgtcctgcgc gccgagaatg cgcagattgc ggaagactgc
     2881 gcggtcgcct gtaacggcaa gggccacggc ctgccccttg ccgctgcctg cgtcgttggc
     2941 gatggtcaga ttcgagatgc tgtcgctgtc ggc
//
LOCUS       DASRBV010000353         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_740476, whole genome shotgun sequence.
ACCESSION   DASRBV010000353 DASRBV010000000
VERSION     DASRBV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740476"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VFA02_13120"
     CDS             complement(<1..1619)
                     /locus_tag="VFA02_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10837.1"
                     /translation="MKPGFYRAGVCILAAALLPGKALHAEVQLPADAAECVRWAVGDL
                     GVALQQKHLSVNDAHVRVSIRAGGPTLPASISRQEQEFSLRVEGRSIAIDAGGAVGAM
                     YGLQELAEQIEQQQPLPWDMLLGRIHATDQKPFLEVRADNMFIHVYPLLLHDLAMWRD
                     YIDMLARNRYNLLDLHGAYDLESTSFPNLYPMLVHVPEYPQAGNEAEQRQNLNDLRAI
                     IVYAASRGVRVALMNYSANNGRGGAFKNEPSVTGVPPEKLADYTAKAVALLIQQLPGL
                     YMLGFRVGESTQPATFYQDAYLKGVRDAGSPNLRLYTRSWQTTKEQLMPIAQAAHNDF
                     DIEIKFNGEHLGLPYQAMQGPEYGSYSYQDYLDVPAPYRIIWQIRANGTHRFWAWENT
                     DFVRRTVRACRLGHARGFTLEPHTAYFSVYPSQYYRSALDIAVYKYIWQKHWMWYYAW
                     GRIGYDPDLPEANLVWQYKQHYGAAGMKAYQAMQQASRIVPLAYAYRCQGPDQRDFSP
                     ETETGNFDTKKGRARQDLLQFAENKPEDRRV"
     gene            complement(1621..2616)
                     /locus_tag="VFA02_13125"
     CDS             complement(1621..2616)
                     /locus_tag="VFA02_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10838.1"
                     /translation="MKAIMLLAVFMLCAFPASAQTACPRLIEFSGEQADPADLDLPDV
                     SGRVLIYYWSELEPQEGKFDFSNTDREILLWTHAGKSVVLRFSAAGWKKWKQPWSQQG
                     TPDWAFRKYHIGKVTEVDGAILPVYWDEGFLKGLEEFLNVVSMHLAEKHLLGKIAFIE
                     IAVGDGGETKPDTEQNKTPEQRAARLSLWQKAGYTNALWYHTIAAIIDIYQRVFPSVP
                     LALMPDASFLGGECALPGGECRENQIIALANQKGVLLQDNGFDRTHIYPSEWHNGHPL
                     ICEQRQSATQMNYPLRDDLDQSVKAGCASLLVFRQDLERTDFQQQVVDFYKHCGH"
     gene            complement(2625..>3084)
                     /locus_tag="VFA02_13130"
     CDS             complement(2625..>3084)
                     /locus_tag="VFA02_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10839.1"
                     /translation="WNLSGIYSIRGGEPVNVYSGTDYALTNSPNQRPAVLHNWILPGS
                     RHRLNKLALTGNTWFDPTAFGAPASGTFSTLGRNAIIGPSRITNNMSAARMFAIPGRE
                     GMTFQFRCDAFGVFNTPNLGTPANKVGSSLGQITSTSGERFLQLSGKLTF"
BASE COUNT          682 a          845 c          854 g          703 t
ORIGIN      
        1 aatacgcgcc ggtcttcagg cttgttttct gcgaattgca gaaggtcctg gcgcgcgcgg
       61 cccttctttg tatcaaagtt accagtctcc gtttccggag agaagtcgcg ctggtccggt
      121 ccctggcaac gataagcgta agcgagcggc acaatcctgc tggcttgctg catggcttga
      181 tacgccttca tgcctgccgc gccgtagtgc tgcttatatt gccagaccaa attggcctcc
      241 ggcagatcgg gatcatatcc gatgcgtccc caggcgtagt accacatcca atgtttctgc
      301 cagatgtatt tgtaaacagc gatgtcgagc gcgctgcggt aatactgtga cggatacaca
      361 ctgaagtaag cggtgtgcgg ctccagggtg aagccacgcg catggcccag cctgcaggcg
      421 cgcactgtgc gccggacaaa gtctgtgttc tcccatgccc agaagcggtg cgtgccattg
      481 gcacgtatct gccagatgat gcggtaaggt gcaggaacat cgaggtagtc ctgatagctg
      541 tagctgccgt actccgggcc ctgcattgcc tgatacggaa ggcccagatg ctcgccattg
      601 aatttaatct cgatatcgaa gtcattgtgc gcagcctgcg caattggcat aagctgctct
      661 tttgttgtct gccagcttcg tgtgtagaga cgcagattcg gactgcctgc gtcccgtact
      721 cctttcagat acgcgtcctg ataaaaagta gcgggctgcg tggattcgcc cacgcggaac
      781 cccaacatgt ataagccggg gagctgctga atcaaaagtg cgacggcctt tgcagtgtag
      841 tcagcgagct tttccggagg cactcccgtt acggaaggct catttttgaa tgcgccgccg
      901 cgtccattgt tggcgctgta gttcatcagg gccacgcgga caccacgaga tgcggcataa
      961 acaatgatcg cgcgcagatc attcaggttc tgccgctgtt cggcttcatt gcctgcctgc
     1021 ggatactccg gaacatgcac cagcatggga tagagattcg gaaagctggt ggattcgaga
     1081 tcataagcgc catgcagatc aagaaggttg taacggttgc gcgcaagcat atcaatgtag
     1141 tcgcgccaca tggcgagatc atgcaacagc aacggataca cgtggatgaa catgttgtcc
     1201 gcgcgtactt ccagaaatgg tttctggtct gtggcgtgga ttctgccgag gagcatgtcc
     1261 cacggcaacg gctgctgctg ctcaatctgc tccgccagtt cctgaaggcc atacatggca
     1321 ccaactgctc cacctgcgtc aatggcgatg gagcgccctt ctacccgaag ggaaaactcc
     1381 tgctcttgcc gggaaataga agcaggcagg gtaggccctc cagcgcgaat ggagacgcgc
     1441 acatgggcat cattcacaga aagatgtttc tgctgaagcg caacacccag atcgcccacg
     1501 gcccagcgca cacactctgc cgcatccgcc ggcagttgga cttccgcgtg caatgctttt
     1561 ccaggcagca atgcggcagc aagaatgcaa actcctgctc tgtagaaacc aggcttcatg
     1621 ttaatgacca cagtgtttgt agaagtccac aacctgttgt tgaaagtccg tgcgctccag
     1681 gtcttgacgg aagacaagca gcgaagcgca tcccgccttc acggactgat caagatcgtc
     1741 gcgcagtggg taattcatct gcgtggcaga ctggcgctgt tcgcagatga gcggatgtcc
     1801 attgtgccat tcggagggat aaatatgtgt gcggtcgaag ccattgtcct gcaaaagcac
     1861 gcctttttgg tttgcaagcg caatgatctg gttttcacgg cattcgccgc caggtaatgc
     1921 gcattctccg ccaagaaaag atgcatctgg catcagcgcc aaagggactg aaggaaatac
     1981 ccgctgataa atatctatga tggcggcaat ggtgtgatac cagagagcat tcgtatatcc
     2041 agccttttgc cagagggaca ggcgcgcggc cctttgttca ggagttttgt tctgctctgt
     2101 gtccggcttg gtttcgccgc catctccaac tgcaatttcg ataaaggcaa ttttgccgag
     2161 cagatgtttt tctgcgaggt gcatggagac aacattcaaa aactcttcca accctttcag
     2221 aaaaccttca tcccagtaga cgggcaaaat ggccccatcc acttctgtta ctttaccgat
     2281 gtggtatttt cggaaggccc aatcgggcgt cccttgttgc gaccacggct gtttccactt
     2341 cttccaccct gccgcagaga aacggaggac cacagatttc ccagcatggg tccacagcag
     2401 aatttctctg tccgtatttg aaaagtcgaa tttgccttct tgcggctcca actccgacca
     2461 gtagtaaatc agcactcgcc cggaaacatc gggcagatcc aaatccgcag ggtctgcctg
     2521 ttcgccggaa aattcaatca atcgcgggca ggcagtctgc gcagatgccg gaaaagcaca
     2581 aagcatgaag acggccagga gcataattgc tttcatctgt ttgcctagaa ggtcagtttt
     2641 ccggaaagct gcaggaaacg ctcgccgctg gtgctggtaa tctgccccag actggagccg
     2701 accttgttcg cgggtgtgcc caggttcggc gtgttgaata cgccaaaggc atcgcagcgg
     2761 aactggaacg tcatgccttc acggcccggt atcgcgaaca tcctggccgc cgacatatta
     2821 ttggtgattc tcgatggccc gatgattgca ttccgcccca gagtgctgaa tgtgcctgag
     2881 gccggtgctc caaaggcggt tgggtcgaac catgtatttc cggtcagcgc cagtttgttc
     2941 agacggtgac ggctgcccgg aagtatccag ttgtgcagca ccgccgggcg ctgattggga
     3001 gaattggtga gagcatagtc cgttccggaa taaacattca ccggctcgcc gccgcgaatg
     3061 gagtagatgc cgctcaggtt ccat
//
LOCUS       DASRBV010000354         6211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_280384, whole genome shotgun sequence.
ACCESSION   DASRBV010000354 DASRBV010000000
VERSION     DASRBV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280384"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFA02_13135"
     CDS             <1..543
                     /locus_tag="VFA02_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZU10840.1"
                     /translation="SHAGGEYATRRAEVEEGTAILRRHRPEIRLLRDATVEDLEKWGH
                     EMPSNVLKRCRHIVTDSLRAVAAADALEANDLALLGRLMAQAHISYRDDFEASCKEAD
                     IMVELAWKQPGCIGARLTGGGFGGCTVNLVESAEAQEFAAAMAEGYEKATGIKPEIYL
                     SHASAGAHAVEEPAKAGADH"
     gene            540..896
                     /locus_tag="VFA02_13140"
     CDS             540..896
                     /locus_tag="VFA02_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10841.1"
                     /translation="MIGIGPVLFAALEIQGYAVSSWIWQFCTPRETEAARDFAALLAR
                     VRAGDEVVIETESAPAVVLRMDVGPRGRLLSESIALAEAHAKELGFEPVLDTDFAADL
                     EDIIRHRRPRDTSVWD"
     gene            887..1024
                     /locus_tag="VFA02_13145"
     CDS             887..1024
                     /locus_tag="VFA02_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10842.1"
                     /translation="MGLILDSSILIAGERRRESVRQIIQRIHSIHGDQESALSVISIV
                     A"
     gene            1418..3172
                     /gene="glgP"
                     /locus_tag="VFA02_13150"
     CDS             1418..3172
                     /gene="glgP"
                     /locus_tag="VFA02_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZU10843.1"
                     /translation="MSLPFSPLHPSRNSRIAYFSMEIAVAPGMPTYSGGLGVLAGDTL
                     RSAADAGLHLSAVTLAHRKGYFRQHLDANGVQTESEQPWKPEEKLIPEEPVVAVTLEG
                     RRIAVRAWRYDVQGITGHIIPIYFLDTDLEQNDPRDRALTDHLYGGDGDYRLRQEALL
                     GIGGVRLLHALGFRAAVYHMNEGHAALLTVALMQDQLRGAPLHTASEDDVAAVRQKCV
                     FTTHTPVPAGHDRFSQEQSSRILGNEIASFLEQRGGVHDGLLNMTYVALTFSRFVNGV
                     AMQHGKVSRSMFPEYEISAITNGVHAVTWTAPAFQTIFDRHMPRWRQDNVTLRYAIDI
                     PEEEIEYAHTQSKQGLMDLVAERTGVRLDPGIFTIGFARRAATYKRADLLFTDPERLA
                     RCAREHGAMQIIYSGKAHPADGPGKEKIRRVIELAHQLSNDSLRIVYLENYEWQIAAH
                     LVSGVDVWLNTPKRPYEASGTSGMKAALNGVPSLSILDGWWIEGWIEGVTGWAIEDYE
                     DESAEAGSLYDHLEHVLLPLYYQQPQQWRRIMRSTIALNGSFFNTQRMLEQYVVNAYF
                     PESLVKQSAQLREPVLAG"
     gene            3233..3475
                     /locus_tag="VFA02_13155"
     CDS             3233..3475
                     /locus_tag="VFA02_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10844.1"
                     /translation="MAEEGKIEKFSAAELSRLRNELMHAGLDSRQAAELVTTFLAMHG
                     YGVNAELVPEILLRLEKAGCSTDCMQAELERVALVM"
     gene            3654..4211
                     /locus_tag="VFA02_13160"
     CDS             3654..4211
                     /locus_tag="VFA02_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU10845.1"
                     /translation="MQEIRQAGARRTARAAASQDRPAINKEHEQALKLYQSAVQLMQE
                     ARFDKARAAFEKLLPVAPPELVERTKVYLAACERQTKQESRSFDSLGEEYDYAISLLN
                     TGDYEDARDHLETILKKDKSADFAHYGLAVLNSMTGQAEECLEHLAAAIQLNPQNRIM
                     ARTDSDFQDMADDPRFTELLYPETA"
     gene            4347..5408
                     /gene="yvcK"
                     /locus_tag="VFA02_13165"
     CDS             4347..5408
                     /gene="yvcK"
                     /locus_tag="VFA02_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZU10846.1"
                     /translation="MKPAILAPQLDVASRHPAQAGLRVVAMGGGTGLSTLLRGLKHYV
                     PPPGGAPPEGHLNLICDLAAVVTVTDDGGSSGRLRKDFNVLPPGDLRNCMVALSEDEH
                     LLSRLFSHRFKSGAGLEGHSFGNLFVIALTELTGDFAEAVRLSAEILATRGHIYPATT
                     ANVSLAAQMDDGEIVRGETNITASRKQIIELTMDPPDAAPLPETLEAIANAHLITIGP
                     GSLYTSVITNLLVQGIPEALAASRGVRVYICNLMTQANESLHLTASEHIERIYEHAGR
                     PIFDYALINTGDVSSELRQRYAAEGAEPIQPDIERIEALGIHCITGDFAAEGDVLRHD
                     ADRVAAQVLQLALETQSPS"
     gene            5573..6082
                     /locus_tag="VFA02_13170"
     CDS             5573..6082
                     /locus_tag="VFA02_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10847.1"
                     /translation="MDLVRVAVVSVASVIAMSAAAQSFPDTVYAPLWNYNGTWKAAVS
                     GKSPDTIVNHCARTGLFFVCEQNVNGQPGALLVFVPVDAEGHYRTNPVQKDGHAYGPG
                     ELEIKGNHWTYLGKNEEGGKTIWYRTTNDFSGNSHIHFEQAKSADKVNWEVLRSGDEV
                     RVDKDNENH"
BASE COUNT         1347 a         1875 c         1722 g         1267 t
ORIGIN      
        1 tcgcacgcag gtggcgaata tgccacgcgg cgcgctgagg tggaagaagg cacggccatc
       61 ctgcgccggc accggcctga gattcggctg ctgcgggatg ctactgtcga agacctggaa
      121 aaatggggcc atgaaatgcc ctccaatgtg cttaagcgct gccgccacat cgttaccgac
      181 agcctgcggg cggtagctgc tgccgatgcg cttgaagcca acgacctcgc cttgcttggc
      241 cgcctgatgg cccaggcaca tatcagctat cgtgatgact ttgaagcaag ctgcaaagag
      301 gccgacatca tggtggaact tgcatggaag cagccgggct gcataggcgc gcgcctcacc
      361 ggaggaggtt tcggcggctg cacggtcaac ctcgtcgaat cagcagaggc gcaggagttt
      421 gccgctgcca tggccgaagg ttatgaaaaa gcgacaggta tcaaaccgga aatttatctg
      481 agccacgcat cggcgggtgc tcatgcagtc gaagaaccag cgaaggccgg cgcagatcat
      541 tgattgggat tggacctgtc ctttttgcag cactcgaaat ccagggttac gctgtatcat
      601 cttggatatg gcagttttgc acaccccggg aaactgaggc ggcgcgcgat ttcgccgcac
      661 tgctggcccg cgtgcgcgca ggtgacgagg tggtcatcga aacggaatca gcgcctgccg
      721 tcgtactgcg aatggatgtt ggaccacgcg gacgcctgtt gtcagagtcc atcgcgctcg
      781 cagaggccca tgcaaaggaa ctcggatttg aaccagtcct ggatacagat tttgctgccg
      841 accttgaaga cattatccgt caccgcaggc cccgtgatac ttcagtatgg gactaatcct
      901 tgactccagc attcttatcg ctggtgagcg gcgccgtgaa agtgtcagac agatcatcca
      961 gcgcattcac agcatccatg gcgatcagga aagcgcgctt tctgtgatca gcatcgtggc
     1021 ctgaatgtgc ttacgctctg accgtgcgca cggtgaagga ccagcgaaga ttgtcgtgag
     1081 tcagtatcat gtctcagcag tttgcaccat accaggaata cgttgttatc ctgaggaggg
     1141 cagcgatgaa ggacctgctt tttgcaattt ctgggtcatg caacaaacct tgcagtgcgg
     1201 ctataagctt gctgtggccg cattgaccat gaacaaaatg gttcatgtaa acccatgaaa
     1261 gtagctcctt tggttgccac ctcagggtgc caacctgata atgcggcctg tttttcataa
     1321 aaaccacaca gggcccttga taaaatcgtt tcagcccatc tctgtgcaga aagaaaatat
     1381 agattccata cattttcgta tgtggagttg ctcctgaatg agtcttcctt tttctcctct
     1441 tcatccttct cgcaattcga gaatcgctta tttttccatg gagattgccg tagcgcccgg
     1501 catgcctacg tacagcggcg gtctgggcgt gcttgccgga gacactcttc gctcggccgc
     1561 tgatgccggc ctgcacctgt ccgctgtcac tctggcccac cgcaaaggct atttccgcca
     1621 gcatcttgac gcgaacggcg tgcagaccga gtccgagcag ccgtggaagc cggaagagaa
     1681 actcattcct gaagaaccgg tcgttgctgt cacactcgaa ggccgccgga tcgccgtgcg
     1741 cgcatggcgt tacgatgtac agggcatcac cgggcacatc atccctatct attttctgga
     1801 tacggacctc gagcaaaatg atccccgcga ccgcgccctc acagaccatc tttacggcgg
     1861 cgatggcgac taccgccttc gccaggaggc ccttctcggc attggcggcg tgcggctgct
     1921 ccatgcgctt ggatttcgtg cagccgttta ccacatgaat gagggccacg cggcgctgtt
     1981 gaccgttgcc ttgatgcagg accagcttcg cggggccccg ctgcacacgg cgagtgagga
     2041 cgatgtggcc gccgtccgcc agaaatgcgt cttcaccacg cacacccccg tgcctgcagg
     2101 acacgaccgc ttctcgcagg agcagtcctc gcgcatcctc gggaacgaga ttgcttcgtt
     2161 cctggagcag cgcggtggcg tccacgatgg cctcctgaac atgacctacg tagcgctcac
     2221 cttctcccgc tttgtgaatg gtgtggccat gcagcacggc aaagtttccc gctcgatgtt
     2281 tccggaatac gagatttccg ccatcaccaa cggcgtccac gcagtcacct ggaccgcgcc
     2341 tgctttccag accatctttg accgccatat gccgcgctgg cggcaggaca acgttaccct
     2401 gcgttacgcc attgatattc cggaagagga aatcgagtac gcgcacacgc agtccaaaca
     2461 ggggctcatg gacctggttg ccgaacgcac aggcgtccgc cttgaccccg gcatcttcac
     2521 cattggcttt gcccgtcgcg ccgccactta caaacgcgcc gacctgctct ttaccgaccc
     2581 tgaacgcctt gcccgctgcg cgcgcgaaca cggcgccatg cagatcatct acagcggcaa
     2641 ggcacacccg gccgacggcc ccggaaaaga gaagatccgc cgcgtaattg agctggcgca
     2701 tcagctcagc aatgattcgc tgcgcattgt ctatctggaa aattatgagt ggcagattgc
     2761 cgcccatctg gtcagcggcg tagacgtttg gctgaacacg cccaagcgcc cgtatgaggc
     2821 gtccggcacc agcggtatga aggcggccct gaacggcgtc cccagcctca gcattctcga
     2881 tggatggtgg attgaaggct ggattgaagg cgtcaccgga tgggccatcg aggactacga
     2941 agatgagtct gcggaggcgg gctctctgta cgaccacctg gaacacgtgc tgctcccgct
     3001 ctattaccag cagccgcagc aatggcggcg cattatgcgc tccaccatcg ccctgaatgg
     3061 ctccttcttc aatacgcagc gcatgctgga gcagtatgtg gtaaatgcct atttccctga
     3121 aagcctggta aaacaatccg cgcagttgcg tgagccggtt ctggccggat aatcccgggc
     3181 aacaaaacat cagcggatgg cttccctatt acggtgcagt gaaaggacca caatggcaga
     3241 agaaggcaag attgaaaaat tttctgcggc tgagctttcc cgtcttcgca atgagctgat
     3301 gcatgctggt ctggattcgc gtcaggccgc cgaactggtc acgacctttc ttgccatgca
     3361 tggatatggc gtgaacgcgg aactcgtccc tgaaatcctt ctccggcttg agaaggccgg
     3421 ctgctccaca gactgcatgc aggccgaact ggagcgggtg gccctggtca tgtgacccgc
     3481 gctggccgct gcaaagaaaa ctctatgttt cgccatcttt tttcctcacc ccggtcccgg
     3541 aatttgactc gcaccccgct tatgcgtata aaaacgagtt caacgggaaa ttttccgcgt
     3601 gcgcgcgcct gtgcgcggca tgattcaaga aaaatcaggg aaaaacattg agtatgcaag
     3661 agatacgtca ggctggtgcc cgtcgcactg ctcgcgctgc cgccagtcag gaccgtccgg
     3721 caatcaacaa agaacacgag caggccctca aactgtatca gagcgcagtg caactgatgc
     3781 aggaagccag gttcgataaa gcgcgcgccg cttttgagaa acttttgccc gttgctcccc
     3841 ccgaactggt cgagcggaca aaggtctatc ttgcggcctg tgaacgccag accaaacagg
     3901 aatcgcgctc cttcgacagc ctgggagagg aatatgatta cgcaatctcc ctgctgaata
     3961 cgggtgatta cgaagatgcg cgcgaccacc ttgaaaccat cctgaaaaag gacaagagtg
     4021 ctgattttgc gcactacggc ctggccgtgc tgaacagcat gaccggacag gctgaggaat
     4081 gcctcgagca tctggccgcc gccatccagt tgaatcccca gaaccgcatt atggcgcgta
     4141 ccgactccga ctttcaggac atggctgacg atccgcgctt taccgagctg ctctatccgg
     4201 aaacggccta aagcagtctt atacagcatg gacagggctg atgccccttg atgccccttg
     4261 atgcgcgttg cggatcaccg ggcttgaaac ccggtgcttc ttgctcacgt tgtttacagg
     4321 ttgtggcccc agatgaagct gcatttatga aaccagccat cctcgcaccc cagctcgacg
     4381 tggcttcccg gcatccggcg caggctggcc tgcgagtggt tgccatgggc ggaggtacag
     4441 ggctgtccac tcttctccgc ggcctgaagc attatgtgcc gcctccaggc ggagcacctc
     4501 ccgaaggcca tctcaacctc atctgcgacc ttgcggccgt cgttaccgtg accgatgatg
     4561 gcggctccag cggccgtctc cgtaaggact ttaacgtgct ccctcccggc gacctgcgca
     4621 actgcatggt cgcgctctct gaggacgaac atctgctttc ccgcctcttc agtcaccgct
     4681 tcaagtccgg cgccggactc gaaggccaca gcttcggcaa tctttttgtc atcgctctga
     4741 ccgagttgac aggcgacttc gccgaggccg tgcggctttc ggcagagatt ctggccactc
     4801 gcggacacat ctaccccgcc accacagcca atgtctcgct tgccgcccag atggatgacg
     4861 gcgagatcgt gcgtggcgaa accaatatca ccgccagcag aaaacagatc atcgagctga
     4921 caatggaccc cccggacgct gcgcctttgc ctgaaacact ggaggccatc gccaacgccc
     4981 acctcatcac catcggcccc ggctcgctct acaccagcgt gatcacaaac cttctcgtgc
     5041 aggggattcc tgaagcgctt gccgcctcgc gcggggtgcg ggtctacatc tgcaacctga
     5101 tgacgcaggc caatgagagc cttcacctta ccgcttccga acacatcgag cgcatctacg
     5161 agcacgccgg aagacccatc ttcgactacg cgcttatcaa cacgggagat gtctccagcg
     5221 agctgcgtca gcgatatgcc gccgaaggcg ccgaacccat ccagccggac attgagcgca
     5281 tcgaagccct gggtatccac tgcatcaccg gcgatttcgc cgcagaaggc gatgtgctgc
     5341 gtcacgatgc cgacagggtt gccgcgcagg tgctccaact ggcgctcgaa acgcaatctc
     5401 cctcataaaa aagcttgcca tccccagaga caaaagcgca ttctgctatt cacccatagc
     5461 attttcacag attgtctgga ccatgctgcg aaacgacagt cgccgcggcc aacagggggc
     5521 cggcgacact gcacagaatc tgtgaaaatg ccgtggcctc tggaggacat ctatggacct
     5581 cgtccgcgtt gccgttgtga gtgtggcttc ggtgattgcc atgagcgctg ccgcgcagtc
     5641 ctttcctgat acggtctatg ctccactctg gaactataac ggcacgtgga aggcggcggt
     5701 ttccggcaaa tctccagaca caatcgtgaa ccattgcgcc cgtaccggcc tgttctttgt
     5761 ctgcgagcag aatgtgaacg gacaaccggg cgccctgctt gtctttgttc ccgtggatgc
     5821 ggagggccat taccgcacca atcctgtgca gaaggacggt cacgcctacg gccccggcga
     5881 gcttgaaatc aaaggaaacc attggaccta tctgggtaaa aatgaagaag gcggaaagac
     5941 catctggtat cgcactacaa acgacttctc cggaaacagc catattcact ttgaacaggc
     6001 aaagtccgcc gacaaagtga actgggaagt cctgcgttca ggagacgagg tgcgagtgga
     6061 caaggacaat gaaaaccatt agccaggcat ggagaccacg tatgcgcttt tgtgcaggca
     6121 gagaaggtca cgtgaacacg cgacgccgga aagttcgctt tcgctaacag catgcacccg
     6181 gctggatgca cgactccgcc aaagacatgg a
//
LOCUS       DASRBV010000355         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_381991, whole genome shotgun sequence.
ACCESSION   DASRBV010000355 DASRBV010000000
VERSION     DASRBV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381991"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1004)
                     /locus_tag="VFA02_13175"
     CDS             complement(456..1004)
                     /locus_tag="VFA02_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3'-deoxyribonucleotidase"
                     /protein_id="HZU10848.1"
                     /translation="MKRIAIDMDEVLADTLAEHIARYNRDFQESVKKSDLEGKWLWEI
                     VPSYRHEQLEGHLRTEDFFEDLPVMPESQRVVKALTQQYEIFIASAAMEFPNSFGPKY
                     RWLCRHFPFLPPTHFIFCGDKSILHADYLIDDQPRQLRRFRGEGILFSAPHNLRIAGF
                     RRVNDWLEVEKLFLTRPKVQYP"
     gene            complement(1001..1759)
                     /locus_tag="VFA02_13180"
     CDS             complement(1001..1759)
                     /locus_tag="VFA02_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HZU10849.1"
                     /translation="MDASLEQLYHLQQIDIESAQLRKAIAALPQHLAAMEEKLRTQKA
                     AVGEAEKAIQAEEAKRRRIESDIKDQQQKIAKFREQSSSVKTNEQFHALQHEISFAEN
                     EIRRLEDTELESMERSEQLEAALRSAKQELERQAAFVEQEKESARVEIAHKEERLITL
                     SKEREALRSQSEPDLLADYDRIAASRGTAVAIVKDQRCFGCQMALRPQMWNQVRQGAR
                     IHCESCGRLLYYEAVADPAGQFADDRPNWNGPAA"
     gene            1929..2852
                     /locus_tag="VFA02_13185"
     CDS             1929..2852
                     /locus_tag="VFA02_13185"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZU10850.1"
                     /translation="MSLQSRITFRKFPTAIIFVLALLLVLPQGHSQTAATRTSLSVAK
                     DGAKATLSVTVKDATGTAVSGGTVSFLSGNASLGSAFVSEAGTATLTLDSLPANTKQI
                     TAVYAGDSSHAASASGSANVQAEDTTLPDFSISASPTSLSLNPGDYAPVVITITPSAT
                     FTQSVTLTISGLPNGSTATFTPQIVTPVNGAAATSTLQIQTSGASGAKNTGHPFGGNA
                     SRIAYALALPGLLALMRRRTGLRLLGMTLLLIASTSGLTACSQRYGYLHHPPAANPGT
                     PAGSYTVTVTAYSNNGGQVTSHNLQLALTVK"
     gene            complement(3050..>3269)
                     /gene="secG"
                     /locus_tag="VFA02_13190"
     CDS             complement(3050..>3269)
                     /gene="secG"
                     /locus_tag="VFA02_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZU10851.1"
                     /translation="KSADLAGAFGGQGSQTAFGPRGAANVLTRITTWCAVIFMLTSIG
                     LTILMQKHTGGHSVLEGTKATQSAPAKK"
BASE COUNT          691 a          930 c          873 g          775 t
ORIGIN      
        1 gcgcctcttt catcgcctgg gcattttcat gcttcgagaa gtcggtaaac ggctcgttgc
       61 gaaactcgcc ctgtggcgag cgcggaagga tgacagtaga gatttcggct gtagccataa
      121 ccgtctagtg tagctcaggc agcagaatgt tttcttccgg tccaggcaac tacaacttcc
      181 gttggttctg ttggctgcaa gacctctgtc aatccttacg aaggtaatgg gttcagtcta
      241 aaagcatctc tttcaatacg atgggccagt cgtggtattg tccatactcc tgtaccccag
      301 ttgatattct gaaataagag ggtaaagata aaaatccgtg ctctgatcag tagataaact
      361 atggatatat tgcgagctaa gttgaagcct taaagtagat aacctatttt attttcaaga
      421 atttagaaaa atctgcactc acatacatag tttatctatg gatactggac ttttggccgc
      481 gtcagaaata gcttttccac ctccagccag tcatttacgc ggcgaaatcc tgcaatccgc
      541 aaattatgtg gcgcggaaaa gaggattcct tctccgcgaa agcgccgcag ttgccggggc
      601 tggtcatcaa tcagatagtc cgcatgcaga atgctcttgt cgccgcagaa aataaaatgg
      661 gtgggcggca gaaatggaaa atggcggcac aaccagcgat atttcgggcc gaatgagttg
      721 ggaaactcca tggccgcgct tgcaataaaa atttcatact gctgcgtaag tgctttgact
      781 acgcgctggc tctccggcat cacaggcagg tcttcgaaga agtcctctgt gcgcagatgg
      841 ccttccaact gctcatgccg gtatgatgga acgatctccc acaaccactt cccctcgagg
      901 tcgctctttt tcacggactc ctgaaaatcg cgattgtagc gcgcaatgtg ctcggcgagc
      961 gtgtcagcca gcacctcgtc catgtcaatg gcgatgcgct tcatgccgca ggtccgttcc
     1021 agttcgggcg gtcatctgcg aactgcccgg ctgggtcagc aacggcctca tagtagagca
     1081 agcgcccgca gctttcacaa tggatgcgcg cgccctgccg cacctggttc cacatctgcg
     1141 gacgaagggc catctggcag ccgaagcagc gctggtcttt tacaatcgcg accgccgttc
     1201 cgcgtgaggc ggcgatgcgg tcataatcag caagcaggtc aggctcggac tggctgcgaa
     1261 gcgcctcgcg ctctttactc aatgtaatga gccgctcctc tttgtgggcg atttccacgc
     1321 gcgctgattc tttttcctgt tcgacaaagg cggcctgccg ctccagttcc tgctttgccg
     1381 agcgcagtgc ggcctccagt tgctcggagc gttccatgct ttccagttca gtgtcttcca
     1441 gccgccggat ttcgttttct gcaaaggaaa tctcatgctg gagcgcgtgg aactgctcgt
     1501 tggtcttgac gctgctggac tgctcgcgga actttgcgat tttctgctgc tggtctttga
     1561 tgtcgctttc gatgcgccgc cgttttgctt cttctgcctg gatggccttt tctgcttctc
     1621 ccaccgcggc cttctgtgtg cgcagttttt cttccatggc ggcaaggtgc tggggaagag
     1681 ctgcgatggc ctttcgcaac tgcgcgcttt cgatgtctat ttgctgaaga tgataaagct
     1741 gctcaagaga tgcatccatt gtctgaccgc agtctatagc atttgagagg acacgcccaa
     1801 accagaaacc acgaaatcca gtttcccgcg tctaacaaat gcttgctggc ggcatcgccg
     1861 tttttgcgcc gcacagatac aattgggtat taaaggattt ccgctcaaaa gggtagagaa
     1921 ccggacgaat gagtttgcag tcacgtatca ctttcaggaa gttcccaacc gccatcatct
     1981 ttgtactggc gcttcttctg gttcttccgc aaggccatag ccagaccgcc gccacacgca
     2041 ccagtctttc cgttgccaaa gacggcgcga aagccacgct ctccgtgacg gtaaaggacg
     2101 ccaccggaac cgccgtttca ggcggaacgg tcagctttct gagcggtaat gcctcgctgg
     2161 gttcggcctt tgtcagcgaa gccggcacgg caacgcttac gcttgattct ctacctgcga
     2221 acacgaaaca aatcactgct gtttatgcgg gagacagcag tcatgcagcg tctgcctcag
     2281 gcagcgcaaa cgtgcaggcg gaagacacga cgttgccgga cttttccata agtgcgagtc
     2341 cgacttcgct gtcattgaat ccgggcgact atgccccggt ggtgattaca atcactccca
     2401 gcgcgacctt tacgcaatcc gttacgctca cgatctccgg cctgccgaat ggctctacag
     2461 ccaccttcac gccgcagatc gtgacaccgg tgaacggagc ggcagctacc agtaccctgc
     2521 aaatccagac ctccggggct tcgggagcaa aaaatactgg ccatcctttt ggcggaaatg
     2581 cttcccggat tgcctatgct ctggctcttc caggacttct ggcccttatg cgccgccgta
     2641 ccggattgcg gctgttgggc atgaccctgc ttctgattgc cagtacctct ggattaacgg
     2701 cctgctcgca gcgctatgga tatctgcacc atcctcctgc ggcgaatccg ggtactccgg
     2761 ctggctccta taccgtcact gtgaccgcat attccaataa cggcggccag gtcacgagtc
     2821 ataacctgca attggctctg acagtaaagt agcctggaca cgaaactacc tcgaggcccc
     2881 cggcacgagg tgtgcgcgtg gatggaagca acaggggcgg cgtcccgcac accgcaaatc
     2941 agccccgtca ttctgatggc aaccgccagg gcctcggaat gcgcaggaac aaacgcgaaa
     3001 ggcagaccct gaggtctgcc ttgttttgtc acatagggga aacctgaatc tatttctttg
     3061 ccggggccga ctgtgtggcc ttggttcctt cgagtacgga atgtccgcct gtgtgcttct
     3121 gcatcaggat cgtcagaccg atcgaggtca gcatgaaaat cacggcgcac caggtagtaa
     3181 tgcgggtgag gacgttggcc gctccgcgcg ggccaaatgc ggtctgcgaa ccctggccgc
     3241 caaaggcgcc agcaagatca gcgctcttg
//
LOCUS       DASRBV010000356         6665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_432412, whole genome shotgun sequence.
ACCESSION   DASRBV010000356 DASRBV010000000
VERSION     DASRBV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432412"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFA02_13195"
     CDS             <1..831
                     /locus_tag="VFA02_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HZU10852.1"
                     /translation="MAAFLMRDSHNTHRWIRFGILSFQPSELAKPALVLFLAWFLQNR
                     MQTIDDWRGTVLPAALPSLVFIVLILKEPDLGTALVCAGVTALMLYLAGMEMKYLGYA
                     ALASLPVLYLMLFRVKWRRDRLLAFVNPEADPLGKGFHIIQSLIAVGTGGLHGLGYME
                     GRQKLFYLPEPHTDYIFANVAEELGLIGALIVVGLFVVLGYRGMRAAILSKDPFARFL
                     AFGFTATILIQAFFNISVVLALVPTKGITLPFISYGGTSLFIMLACIGVLLNITREIE
                     "
     gene            1189..1386
                     /gene="rpmI"
                     /locus_tag="VFA02_13200"
     CDS             1189..1386
                     /gene="rpmI"
                     /locus_tag="VFA02_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZU10853.1"
                     /translation="MPKLKTHRGAAKRFKKTATGKIKRGQSKMRHILTSKEVKTKRRL
                     SAPAYVSDADHAKVARMIPYA"
     gene            1522..1890
                     /gene="rplT"
                     /locus_tag="VFA02_13205"
     CDS             1522..1890
                     /gene="rplT"
                     /locus_tag="VFA02_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZU10854.1"
                     /translation="MPRVKRGTKRTDRRKKILKRAKGYFLTKSKLYQAAQEAVERGLK
                     FAYSGRRQKKRQFRSLWIVRINAAAQQNGISYSQFINGLKKAGIELDRKVLADIAVND
                     APGFTALVEQAKAALTQAAA"
     gene            2143..2346
                     /locus_tag="VFA02_13210"
     CDS             2143..2346
                     /locus_tag="VFA02_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZU10855.1"
                     /translation="MEQGTVKWFNDAKGFGFITRDANNEDIFVHFSAINSNGFRSLQE
                     GQRVQFNVVKGAKGFQAENVQAA"
     gene            complement(2398..3711)
                     /locus_tag="VFA02_13215"
     CDS             complement(2398..3711)
                     /locus_tag="VFA02_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10856.1"
                     /translation="MREPAQRQSQTGAWEERDLIRRLCHALDIAKLTVEHLAPGGYVD
                     LHDSEKNIRAEKVLSETAVLLLAASQVAQSEVQDRVKSLIVCLAAHARSKRALIGMCV
                     EPSVAWEYALGHLCLNRLGCPDSDFDTLLLHALHAQAHAGRERVPHRMLEKEWAALGL
                     RDLAEDDRRIQMSRLSVLNCPMDLLSCRREDMYAFTHALMYVTDFGIRPVPLPRPREV
                     VLAEAEALLAYCLDEQDYDLAGEILLSWPLTGKSWSPAAAFAFRVLAHVEDKAGFLPT
                     PGTRISEIEVRSGVERTQYRLATAYHTAYVMGLLCAASLRPGHAPPASFACKDARPGG
                     TAHILRVLDADGKHPHWQEEFNQLTSHEADALAGLLVQVSLRRKTMQRDYSGLLQALE
                     VACDLDLSDMPSAVQAAELLDRLAAYSEIEQKMAATAPADCLEYA"
     gene            complement(3730..3975)
                     /locus_tag="VFA02_13220"
     CDS             complement(3730..3975)
                     /locus_tag="VFA02_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10857.1"
                     /translation="MGNIDQHRKIVRQLLDTKAVDFAAIGKAVTELGPSLAVAEEPWD
                     FFCGTNRIFIHIYRIYNPGTPVEDLGALAANAGELRQ"
     gene            complement(4152..4793)
                     /locus_tag="VFA02_13225"
     CDS             complement(4152..4793)
                     /locus_tag="VFA02_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU10858.1"
                     /translation="MGDLMLANYRSWMKSSFFFATLFGLLSVPAMQAQNPAHALHNSD
                     VSLSAFGQFTSSSNGDGVKVDPTKSIGGQVAFRHVYHPWMGFEASYGYTRFTDRYSST
                     VFPVQHNVHEFGGSYLVSAPGLLGIQPFGLAGVSALLYSPTLNGGQRASAQAKAAINF
                     GVGANLPLFTSHFGARVQYRGLYHQTPDFADPRYKTGTWRLTSEPMVGLYVKF"
     gene            4881..5744
                     /gene="ribH"
                     /locus_tag="VFA02_13230"
     CDS             4881..5744
                     /gene="ribH"
                     /locus_tag="VFA02_13230"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZU10859.1"
                     /translation="MIKAITVVRQTSTPAVYDKLAGFFSALGFEQGKGWENAQSRGSS
                     FLAPLGNIEFVDGTLSVPAEILIEVTSLDSVREVAQKWLARNSPEARITEISETYWKS
                     RLFAVEPEPGITFAFWEWLDPLQGRPVALEGDLSAEKMRFAIVVSRWNAVITDRLLQG
                     ALDALLRSGAKKEDVSIIRVPGAWEIPSAARKAAESGNFDAIITLGVLLRGETAHYEA
                     IYNEVARGIGQSQQETGVPHTFGVLTCESLEQALDRAGLKMGNKGFEAAAAAIEMVSL
                     HRKIEAGAVRK"
     gene            5741..6169
                     /gene="nusB"
                     /locus_tag="VFA02_13235"
     CDS             5741..6169
                     /gene="nusB"
                     /locus_tag="VFA02_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZU10860.1"
                     /translation="MSAPGKRRKARELAMQMLFQLDMGKQTPDQVRTTFWKAREESET
                     GPDVRGFAEDIFRIAVSREAEVDGLIEKHSANWRLDRMGSVDRNVLRMAVAEMIGFPG
                     TPAPIIINEALEIARRYSAPESIHFLNGVLDAVAKARLSA"
     gene            complement(6170..6622)
                     /locus_tag="VFA02_13240"
     CDS             complement(6170..6622)
                     /locus_tag="VFA02_13240"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HZU10861.1"
                     /translation="MSTIPIIAGSLNDQQRAVFYAQMSAVQKDEVVGVLLALFLGSFG
                     AHHFYLRRNGLGILYVLFFWTGIPAIAGFIECFFMPGRVRQYNMEQATLLAASLQGAV
                     PGPIQVSAQGVAETVSAAPTITTACSTCGAPLVAGVKFCSRCGTSVPG"
BASE COUNT         1517 a         1920 c         1818 g         1410 t
ORIGIN      
        1 atggccgcct tcctgatgcg tgattcacac aacacgcacc gctggatccg tttcggcatt
       61 ctgtcctttc agccatcgga actggccaag cctgcgctcg tgctttttct cgcatggttt
      121 ctgcagaacc gaatgcagac cattgacgac tggcgcggga ctgtcctgcc cgccgctctg
      181 cccagcctcg tctttattgt gctgatactg aaggagccgg acctgggcac ggcgctcgtc
      241 tgcgcgggag tcaccgccct gatgctctat ctcgccggaa tggagatgaa gtatctgggc
      301 tacgccgcgc tggcgtccct gcccgtgctt tatctcatgc tctttcgcgt gaagtggcgg
      361 cgcgaccgcc tgctggcatt tgtgaatccc gaggccgacc ccttaggcaa aggcttccat
      421 attattcagt cgctgattgc tgtcggcact ggaggactcc acggcctcgg ctacatggaa
      481 ggccgccaga agctctttta tttgcccgag ccgcacacgg actatatctt tgccaatgtc
      541 gccgaagaac tggggctgat aggcgccctt atcgttgttg ggctattcgt tgtgctcggc
      601 tatcgcggga tgcgcgctgc catcctctcc aaagacccct ttgcccgctt tctggccttt
      661 ggattcacag caaccatcct gattcaggcc ttcttcaata tcagcgttgt gctggccctg
      721 gtcccgacga agggaattac actgccgttc atctcctatg gcggaacatc actcttcatc
      781 atgctggcct gcatcggtgt gctgctgaat attacgcgcg aaattgaata gggatgaaca
      841 gggatacagc acgacgtcag cttttgtctg gcccttgaag gacctctttt cgacgcttga
      901 gattgatcct cagcgtgctt ccgagcgcgt tctgggccgg gagaatttgt ggcctgggtc
      961 agctcaattg cgctgctgac ggccggtgag gtcttggcct tgagggcaac tcgtatctcc
     1021 cccgccgcag ttctttggca tgagtaactg cctttacaac atctgacggc ctgagcgcgg
     1081 gccagattcg attgtggtat gacatctcaa tacctcactg gccacaacgc tccacttccc
     1141 gtatactgat atctgctgta cttatagctt tttcaagcag gacaaaccat gcccaaactg
     1201 aagacacaca ggggcgcggc caagcgcttc aagaagacgg ccacgggcaa aatcaagcgg
     1261 ggccagtcga agatgcgcca tatcctcacg tcgaaggaag tcaagaccaa gcgcaggctc
     1321 tctgcccccg cttatgtctc tgacgccgac cacgcgaagg tcgcccgcat gatcccgtat
     1381 gcctgataca ggtagctggt aaagcgttcc agccgccccg gtcgcaagac cgagccatca
     1441 ggtgagaaaa gcgagtgaag aggtcaaagc gaaagctcct gcctccagcc gcacaacgca
     1501 acgcaaaaag gagaaacaac catgccacgc gtaaaacgtg ggaccaaacg tactgatcgg
     1561 cgcaaaaaaa tcctcaagcg cgccaaaggt tacttcctca caaaatcgaa gctctatcaa
     1621 gccgcacaag aggccgtcga gcgtggactc aagttcgcct actcgggccg ccgccagaag
     1681 aagcgccagt tccgttcgct ctggatcgtg cgcatcaacg ccgctgccca gcagaatggc
     1741 atcagctact cgcagttcat caacggcctc aaaaaggctg gaatcgaact cgaccgcaag
     1801 gtgctggctg acatcgccgt caatgacgca cccggcttta ccgccctcgt cgaacaggca
     1861 aaggccgccc ttacgcaggc cgccgcataa agccgatggc cgcgaactgt agccgctata
     1921 cataggtaaa caaggaaagg taaacaagga aaaaggacgc ggaaatcgcg tcctttttcc
     1981 ttgtttttgc aatactttca acaatttcgc ccgcaaaggg cgtacaataa aaacacgctc
     2041 gattccgagt gggttcttgt aagcacggaa tgcactatcg cactaccctg cccttttacc
     2101 tccccgtttt cagcgacatc tcaactcaaa ggaatacccc aaatggaaca gggtacagta
     2161 aagtggttta acgacgccaa aggttttggc tttattacac gcgatgcaaa caacgaagat
     2221 atcttcgtgc atttttcggc gatcaactcc aatggattcc gcagcttgca ggaaggccaa
     2281 cgcgtccagt tcaacgtagt gaagggcgca aagggcttcc aggccgaaaa cgttcaggct
     2341 gcttaatcac tggttattca cacgggccct ggtcatgatg accagggtca ttcgttttta
     2401 agcatattcc aggcagtccg ccggggccgt cgccgccatc ttttgttcta tctccgaata
     2461 tgcggcaaga cggtccagca actccgctgc ctggactgca gatggcatat cagacaaatc
     2521 cagatcgcag gccacttcaa gcgcctgtag aaggccacta tagtcccgct gcattgtttt
     2581 tctgcgcagc gaaacctgta cgagaagtcc cgcaagtgca tctgcttcat gggaagtaag
     2641 ctgattgaat tcctcctgcc aatgcggatg ctttccatct gcatcgagaa cgcgaaggat
     2701 atgggcggtg cctcccggtc ttgcatcctt acatgcgaag gatgcgggcg gcgcgtgtcc
     2761 cggacgcagc gaagcggcgc acagcaggcc catcacatag gcagtgtgat atgcagtcgc
     2821 cagcctgtac tgtgtccgtt ccactcctga acgcacttca atctcactga tacgagttcc
     2881 cggagtaggc aggaagccgg ccttgtcctc aacatgcgcg agcacgcgaa aggcgaatgc
     2941 cgccgcagga ctccacgatt ttcctgtaag tggccagctt aagagaatct cgcctgcaag
     3001 atcatagtcc tgctcgtcca ggcaataggc cagcagcgct tcggcttcag ccaggaccac
     3061 ctcacgtggg cgcggcagcg gcactgggcg gatgccgaaa tcagtgacgt acatcagcgc
     3121 atgagtaaag gcgtacatgt cctcgcgcct gcatgacagc aggtccatgg gacaattcag
     3181 cacagacaat cgggacatct ggatacggcg gtcatcctct gcgagatccc ggagccccag
     3241 tgccgcccat tctttttcca acatgcggtg cggaacgcgc tcacgtccgg catgggcctg
     3301 tgcatgaagg gcgtgcagca gcaacgtatc aaaatcagaa tcagggcatc caaggcgatt
     3361 caggcaaaga tgccccaatg catattccca cgcgacagat ggctctacgc acatgccgat
     3421 cagggcgcgc ttgctgcgcg catgagcagc aagacaaaca ataaggcttt ttaccctgtc
     3481 ctgaacttca gactgcgcaa cctgagaggc tgcgagaagc agtacagcag tttcagaaag
     3541 gaccttttct gcgcgaatat ttttttcact atcatgcagg tccacgtatc caccgggggc
     3601 cagatgctcc acagtcagtt ttgcgatatc gagagcatgg cagaggcggc gaatgagatc
     3661 gcgttcctcc catgcgccag tttggctttg ccgttgagcg ggttctctca tttctgccat
     3721 gcgctcctgc tattggcgta attcgccggc attcgccgcg agcgcgccca ggtcttctac
     3781 cggagttccc ggattgtaga tacggtagat atggatgaaa atgcggttgg tgccacagaa
     3841 aaagtcccac ggctcctctg ccacggcaag cgatggacct agttcggtta cggccttgcc
     3901 gatggcagcg aagtcaacgg cctttgtatc aagcaattgc ctgacgattt ttctgtgctg
     3961 atcaatattt cccatgtgaa ttttctcctt gtctctcgcg gaattgcgta gaggggagac
     4021 cgcgactatg aggatggtgc ggggacagag aaatgtgttt catcgcaaaa aacccgccgc
     4081 aaatggcggc gggcaaataa gatgttgaaa catgctcaac tctcagcagt aagtcttcgg
     4141 gacacggaca tctaaaactt cacatacagc cccaccattg gctcgctggt cagccgccat
     4201 gtcccagtct tgtaccgcgg atccgcaaaa tccggtgtct ggtggtacag tccccggtac
     4261 tgcacgcgcg ccccaaaatg cgaggtgaat aaagggagat ttgcacccac cccgaagttg
     4321 atggcagcct ttgcctgtgc gctcgcgcgc tgcccgccat tcaatgtagg cgaatacaga
     4381 agcgcactga ctccagccag gccgaagggt tggatgccga gaaggccggg cgcgctcacc
     4441 agatacgatc cgccaaactc atgcacgtta tgctgcacgg gaaacactgt cgaagaatag
     4501 cggtcagtga agcgcgtata tccataactg gcctcaaagc ccatccacgg atggtaaaca
     4561 tgacggaatg ccacctgtcc acctatagat ttcgtgggat ccaccttcac tccatcacca
     4621 tttgacgaac tcgtgaactg gccaaaggcg ctcagcgaaa catcgctatt gtgcaaagca
     4681 tgggcgggat tttgcgcctg catcgcagga acagaaagca atccaaacag cgtcgcaaag
     4741 aaaaatgaag acttcatcca actccggtaa tttgccaaca tcaggtctcc cattgtcata
     4801 gtagacgatt gttgcgtttg aaggagagtc actgtttgcc caatttccgc tttgccgcca
     4861 tgcgctgata aagtcgaagt atgatcaagg ccattaccgt agtgcgtcag accagcactc
     4921 cggctgtata tgacaagctt gccggcttct tttccgcgct gggatttgaa caaggcaaag
     4981 ggtgggaaaa tgcccagagc cgaggcagct cttttcttgc gccgctcggc aacattgaat
     5041 ttgtagatgg aacactttct gttccagcag agattcttat tgaggtcacc agcctcgatt
     5101 ctgtccgcga ggtcgcgcag aagtggttgg cccggaactc gcctgaggcc cgtatcacgg
     5161 aaatctccga gacctactgg aaatcgcgcc tgttcgctgt ggaaccggag ccgggcatta
     5221 ctttcgcatt ctgggagtgg ctcgacccgc tccagggcag gcccgtggcg ctggagggcg
     5281 atctttccgc cgaaaagatg cgctttgcca tcgtcgtttc acggtggaac gccgtcatca
     5341 ccgaccggct gctgcaaggg gcactcgatg ctctgctgcg cagtggcgcg aagaaagaag
     5401 atgtaagcat cattcgtgtt cccggggcct gggagattcc gtcggctgcc cgcaaggccg
     5461 ccgaatcagg aaacttcgac gccattatca ccctcggtgt tctcttgcgc ggggaaacgg
     5521 cgcactacga agccatctac aacgaagtgg cgcgcggcat cggtcaatcg cagcaggaga
     5581 cgggcgttcc acatacgttt ggcgtgctta cctgcgaatc gcttgaacag gcgctcgacc
     5641 gcgccggcct gaagatgggc aacaaaggct tcgaggccgc tgcagctgcg attgagatgg
     5701 tctcgttgca ccgcaagata gaggccggag cagtgagaaa atgagcgctc ccggaaaacg
     5761 ccgcaaggca cgcgagcttg ccatgcagat gctctttcag ctcgacatgg gtaagcagac
     5821 acccgaccag gtccgcacga ctttctggaa agcgcgggaa gagtccgaga ccggtcccga
     5881 tgtgcgcggc tttgctgagg acatctttcg cattgccgtt tcgcgggaag cagaagtaga
     5941 tggcctgatc gaaaaacact cggccaactg gcggctggac cgcatgggta gtgtggaccg
     6001 caatgttctg cgcatggccg tcgccgagat gattggcttc cccgggacgc ctgcgcccat
     6061 catcatcaat gaggcgctgg aaattgcccg acgctactct gcgccggaat ccatccattt
     6121 tctgaacggc gtactggatg cagtggcaaa agcgcggctc tctgcctgat tagccgggca
     6181 cagatgtacc gcagcggctg cagaatttca ccccggcaac cagaggcgct ccgcaggtag
     6241 agcatgcagt ggtgatggta ggtgctgctg atactgtttc tgccacaccc tgggccgaaa
     6301 cctggatcgg acctgggacc gcgccttgca ggctggcggc gagcagagtg gcctgttcca
     6361 tgttgtattg gcgcacacgt ccgggcatga agaagcattc aataaaacca gcgatggctg
     6421 gaatgccggt ccagaagaag aggacgtaga ggatgcccaa tccattgcgg cgcagataaa
     6481 agtggtgcgc gccgaagctc cccagaaaca acgcaagtag cacgcccacc acttcatctt
     6541 tctgcacggc tgacatctgc gcataaaaga ccgcgcgctg ctgatcattc agacttcctg
     6601 cgatgatggg aatcgtgctc atggggccct gctccttacc ttcttatcaa cgatgataca
     6661 tacgc
//
LOCUS       DASRBV010000357         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_699506, whole genome shotgun sequence.
ACCESSION   DASRBV010000357 DASRBV010000000
VERSION     DASRBV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699506"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1458)
                     /locus_tag="VFA02_13245"
     CDS             complement(106..1458)
                     /locus_tag="VFA02_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZU10862.1"
                     /translation="MKSSNRVLFAFAFALFCAACAQALDRQSNADYHARRVALAHKLN
                     GGVAVLFAAEEPLLDFMPYRQDADFYYLTGWNEPGAALLIEAPVAAQAGHPARPYREI
                     MFLPTRNLRMELYTGRKLDAAAPDAPQITGVDEVKPMGDLAVTLNQLAAENRAAMYHI
                     WSEPDTEQAKSLVAWTATTLGMESAPPLSDVRGLIAQLRAVKDAGELALLRKASDASV
                     QAQFAMMKSVHPGQKERTVAGIILEKLMENGCERPSYAPIVGSGEKSTELHYADNSQT
                     MQDGDVVVVDAAGEYSMYASDITRTVPVNGHFTGRQREIYNIVLGAQKAAIEGFVAGK
                     SRINDPQHKYPDSLDTIAWNYMNAHGKDLHGQPLGKYFIHGIGHSVGIDVHDPYDYSQ
                     PVQKGMVFTIEPGIYIPEEKTGVRIEDVFYVNQDGKLECLTCALPKEAEAVEAEMHRK
                     "
     gene            1546..1806
                     /locus_tag="VFA02_13250"
     CDS             1546..1806
                     /locus_tag="VFA02_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZU10863.1"
                     /translation="MEINIHDAKTNLSKLIQKAENGEEVIIARAGKPAVKLVPVPARP
                     RRRIIGSAKGKVWISDDFNSPETNMEIEDLFYNSQLFPDEEK"
     gene            1803..2189
                     /locus_tag="VFA02_13255"
     CDS             1803..2189
                     /locus_tag="VFA02_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU10864.1"
                     /translation="MRALLDTHTFLWWINDDPRLSARVKRLIADPKSDFLIGYSSLWE
                     AAIKIQKGTLKVPGRSIAFLLQEMEKNAFRLLPLRTEHLLRLEALEPHHRDPFDRMLI
                     AQAIEEDIPLLTKDPHFHHYPIKIIW"
     gene            complement(2198..3823)
                     /locus_tag="VFA02_13260"
     CDS             complement(2198..3823)
                     /locus_tag="VFA02_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HZU10865.1"
                     /translation="MHSCISLLAQANQHSLSHYLRSHYGDRTFEHLYRWNWFDTSLLI
                     PYFAVMIVLAFYGLHRYQLVYLYYKHRKNAAKEPPERFEQLPRVTVQLPIFNEQFVID
                     RLIDACCNLDYPRELLEIQVLDDSTDETKEVARNLVEQYRNAGNPIVYIHREDRHGFK
                     AGALDHGLKTATGEFVAIFDADFVPPRDWLMQVIHHFAEPDVGMVQTRWTHLNRDYSF
                     LTQVEAILLDGHFVLEHGGRSRAQVFFNFNGTAGMWRRKAIEEAGGWQHDTLTEDTDL
                     SYRAQLKGWRFKYLQDVECPAEVPIEMTAFKTQQARWAKGLIQTSKKILPRVFRSDAP
                     FHTKLEAWYHLTANISYPLMVVLSTLLMPAMIIRFYQGWFQMLLIDVPLFMASTFSIS
                     SFYLVSQKELFPRTWYKTFLYLPFLMALGIGLTVTNTKAVMEALFGIQSSFKRTPKYR
                     VQKRGEKSKAAKYRKRLGIVPWIELLIGCYFALTIWYAITNENYFTVPFLFIFVMGYW
                     YTGLLSLLQGRFERWRSGANFEESSPKPYPVGV"
BASE COUNT          907 a         1064 c         1021 g          859 t
ORIGIN      
        1 caacggaggg ggcaaatttc ctcaggaccg cggctgcaat gccggaaatt ccaacgacag
       61 tgacaaatcc cgccagaatt gcgaaaaaag attgcgcgat ggccttcatt tcctgtgcat
      121 ttccgcttca acggcttcgg cttccttcgg tagcgcgcac gtaaggcact ccagtttgcc
      181 gtcctggttt acatagaaga cgtcttcaat tctcacgccg gtcttttctt cgggaatata
      241 aatccccggc tcgatggtga agaccatccc tttctgaacc ggctgggagt agtcatacgg
      301 atcatgcaca tcaatgccga cggaatgtcc aatcccatga atgaagtatt ttccaagagg
      361 ctgcccatgc aggtccttgc cgtgagcatt catgtagttc caggcgatgg tgtcgaggga
      421 gtcaggatac ttgtgctgcg gatcattgat gcgcgatttt cctgcaacaa agccttcgat
      481 ggcggccttc tgcgcgccaa gcacaatgtt gtaaatttcg cgctgacggc cggtaaagtg
      541 tccattcacc gggaccgtgc gcgtaatgtc tgacgcatac atcgaatact cgccggccgc
      601 gtccacaaca acgacatcgc cgtcctgcat cgtctgactg ttgtcggcat agtgcagttc
      661 ggtggatttt tcacccgagc caacaatggg agcatacgat ggacgctcgc agccgttttc
      721 catcagcttt tcaagaatga tgccggcgac cgtgcgctcc ttctggcccg gatgcacgga
      781 cttcatcatg gcaaactgcg cctgaacaga agcgtccgag gccttacgca gaagagccag
      841 ttcgcctgca tccttaacgg cccgcaactg agcaatcagg ccgcgcacat cactgagcgg
      901 aggggcgctt tccatgccca gcgttgtcgc cgtccatgcc accagcgact ttgcctgctc
      961 tgtgtctggt tccgaccaga tgtgatacat agccgcacgg ttttctgctg caagctgatt
     1021 gagcgtgacg gcaagatcgc ccatcggctt cacttcatcc acgcctgtta tctgcggagc
     1081 atccggcgca gcagcatcga gtttcctgcc ggtgtaaagt tccatgcgca gattgcgggt
     1141 gggcaaaaac atgatctcgc ggtagggacg cgcaggatgt cctgcctggg ccgccacggg
     1201 tgcctcgatc aggagcgccg cccctggctc attccatcct gtcaggtagt agaaatccgc
     1261 gtcctggcgg tacggcatga agtcgagcag cggctcttcg gctgcaaaga gcacagcaac
     1321 accgccattc agcttatggg ccagagccac acgccgcgcg tgataatcag cattcgattg
     1381 gcggtcgagg gcctgcgcgc aggcagcaca aaacagagca aacgcaaatg caaacagaac
     1441 acgattcgag gatttcatca ctcagtaagt ctaacgtgct gtatatctat gtgaattttg
     1501 ctataggtcc tttacctagc taaccgtatt agctaacatg aatctatgga gatcaacatc
     1561 cacgacgcca agaccaatct ctcaaagctg attcagaagg ccgagaatgg tgaagaggtc
     1621 attattgccc gtgcgggaaa gcctgcggtg aagctcgttc ctgtgccagc cagacccagg
     1681 cgccgcatca ttggctcggc gaaaggaaaa gtctggattt cagatgattt caattctcct
     1741 gaaaccaaca tggaaatcga agatttgttt tacaacagcc agctttttcc ggatgaagaa
     1801 aagtgagggc actccttgac actcacactt tcttatggtg gatcaatgat gatccacgcc
     1861 tgtcagcacg cgtcaagcgc ttgatcgcag acccgaaatc tgattttctt attggctaca
     1921 gttctctttg ggaagcagcc atcaaaatcc agaaaggcac ccttaaagta cccgggagat
     1981 ctattgcctt tcttttgcaa gaaatggaga agaacgcttt tcgtctgctg cccctacgta
     2041 cggaacacct tttacgactg gaagcattgg aaccacatca tcgcgatccc tttgaccgga
     2101 tgctgatcgc acaggcaatc gaggaagata ttcccttact caccaaggac ccccactttc
     2161 atcattatcc aataaagatc atctggtgag tttatattca cacgcccact ggatatggct
     2221 ttggcgacga ctcctcgaaa tttgcgccgg agcgccagcg ctcaaaccgc ccctgcaaca
     2281 gcgacagcag ccccgtgtac cagtagccca tcacaaaaat aaacaggaac ggcacggtga
     2341 agtagttctc attggtgatg gcgtaccaga tggtcaaagc aaagtagcag ccaatgagca
     2401 gctcaatcca cgggacaatg cccaggcgct tgcggtattt cgctgccttt gatttctcgc
     2461 cgcgcttctg cacgcggtat tttggcgtgc gcttaaacga gctttggata ccgaagagcg
     2521 cttccattac tgctttggta ttggtcaccg tcaacccgat gcccagcgcc atcagaaacg
     2581 gcaggtacag aaacgttttg taccaggtac gcgggaaaag ttccttttgc gataccagat
     2641 aaaacgacga gatcgaaaac gtggacgcca taaacagcgg cacgtcaatc agcagcatct
     2701 ggaaccatcc ctgatagaag cggatgatca tggccggcat gagaagcgtg gaaagcacca
     2761 ccatcagcgg atagctgatg tttgccgtca ggtggtacca cgcttccagc ttggtatgga
     2821 acggggcatc gcttctgaaa acacgcggca ggattttctt cgaggtctga atcaacccct
     2881 tggcccagcg cgcctgctgc gttttgaagg cggtcatctc gataggcact tctgccgggc
     2941 actcgacatc ctggagatac ttgaaccgcc agcccttcaa ctgggcgcga tagctcaggt
     3001 cagtatcttc ggtcagagta tcgtgttgcc atcctccggc ctcttcaatg gccttgcggc
     3061 gccacatccc ggctgtcccg ttgaagttga agaagacctg cgcgcgtgat cgtccgccat
     3121 gttccagcac aaagtggccg tccagcagga tggcttctac ctgcgtcaga aaactatagt
     3181 cgcggttcag gtgcgtccag cgcgtctgca ccatgcccac gtccggctcg gcaaagtggt
     3241 ggatgacctg catcagccag tcgcgcgggg gcacaaagtc ggcatcaaaa attgcgacga
     3301 attcgcctgt ggcggtcttc agtccgtggt caagcgcccc ggccttgaag ccgtgccggt
     3361 cttcgcggtg aatataaacg atgggattgc ccgcattccg gtattgctcg accaggttgc
     3421 gcgccacctc tttggtttcg tcggtcgaat cgtcgagcac ctggatttcc agtaattccc
     3481 gcggataatc gaggttgcag caggcatcta tcagccggtc aatcacaaac tgctcattga
     3541 aaatcgggag ctggacggtg acccgcggaa gctgctcgaa acgttcaggc ggttccttcg
     3601 cagcgttttt acggtgcttg taatagagat acacaagctg atagcggtgc aatccataga
     3661 acgcaagcac gatcatcacc gcgaaatatg ggatgagaag cgaggtatca aaccagttcc
     3721 agcggtaaag gtgctcgaag gtgcggtctc cgtaatggga acgaagataa tgcgacaggc
     3781 tgtgctggtt cgcctgggca agcagcgaaa tgcagctatg cattgcagga atggcagtct
     3841 gggtaagcag c
//
LOCUS       DASRBV010000358         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_234146, whole genome shotgun sequence.
ACCESSION   DASRBV010000358 DASRBV010000000
VERSION     DASRBV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234146"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VFA02_13265"
     CDS             <1..1276
                     /locus_tag="VFA02_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10866.1"
                     /translation="LRVNYSYGFPAPLGGGPYDRSASLPLTDPSKLNFFAVAGSAGTP
                     TLESAVALWNQQPSGATGLIVLPNFESFNIDLTGTNAIRLREESQLFIVAAETHSASP
                     QEITFTNSCATLTGNIEVQSTAVNSTPAGQLVFSGVWIAGQLTITGQAANVQLMDCTL
                     VPGLALSTDGEPQFPGEPSIIVSATEAALSLTRSISGPIAAAPGGTTRICSSVVDSTS
                     PCCIAYAGNDLASAGADLHVEDSTIIGKVRTRTMTMASDTIFYARLARRDPWPAPVWC
                     SRQQAGCMRFCFVPANSITPRRYKCLPVDAASEAALRPDFITRRYGDPSYMLLSGFNP
                     LAIWQGADNGSQMGVYNQLQETEAVRNVQLRAPEYLPFGLESGIFLVPSRPIVEQPLP
                     PTHAYYGPQRARTCCDDEAEDHGFTGIGANLI"
     gene            1291..3777
                     /locus_tag="VFA02_13270"
     CDS             1291..3777
                     /locus_tag="VFA02_13270"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HZU10867.1"
                     /translation="MKGDFSRIRFSPEKQYTAVIEQQGRVALDADANEQIAINEYLSR
                     AETVDVIGEYGAPANDAGFAISVNNGEIFIGTGRYYVEGLMCENTNSNLTLSTQPFLI
                     DPNLDEAGLLQELQRYKGEASIRVYLQVWQRLVTALDDPCLREPALGQADTTARLQTV
                     WRVVASLVAPANLKPGPIRQPIPGERLNRTNPVLSSVAAHPVAGIGTHPITSPVSVST
                     GVSGTSGTAPLPIHLLTCCEQMYLDATELSTGKLSAQTSGTSDTCGCQPIPAAGYRGL
                     ENQLYRVEIHTGGDETTATFKWSRENGSVVSAVLGVSGATVQVDSLGPDANLGFQANQ
                     WVEIYDDTFLFGPTPNQPGNLYQIQSIDPASNSITMYTTVAAVETWRNARIRRWDQTG
                     ASATSNGIPLSAGTWLDLENGIQISFAAGTYQSGNYWTIPARTASGQIDWPPCGSDGS
                     PWQLAKSVRVYSAPLACIHWDIKKEAFYVEDCRRLFSPLTDLTAPVTPQAMHVTQISW
                     TNDDIITLDQLVASGLTVTLDGNPSGPVNSANFVVDFEIAAPPPNTDSAKEFAAIFQR
                     AIFEGAPSTILRGLTITDSLITVNNGAILWQLPYLEAGYEQRITILYLNALLSYGARA
                     GWFARARVRLLGRTIFSGSGGNQIFLDGQSYGQAALRADGTTPRIDLQLPSGNDEKAS
                     DFESWFYVAPTLVLSSLTVNYPALTVVINANNQVTGVTAPNAAGAPQPVVPQATLTVN
                     YPAIADTTVSLALTGTSGVGTIASVPSTVTINRNTTSVSIPISVLGNPGANTTYTFQI
                     TASITSAVGLTSQLTASFTVTGVPPNIIIQ"
     gene            3798..4718
                     /locus_tag="VFA02_13275"
     CDS             3798..4718
                     /locus_tag="VFA02_13275"
                     /inference="COORDINATES: protein motif:HMM:NF042586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6734 family protein"
                     /protein_id="HZU10868.1"
                     /translation="MRAVWSFWSKPFQSAKGRTWKTHFHHLLSWGLSLRLARRHYPET
                     VLVTDRAGKALLVDHLGLSFTHVSTELERLRHADPSFWASGKLVAYSIQDQPFLHLDT
                     DVFLWARLPSSVEQSPMFAQYPEQHSTDQHYSPRTVERLFERHQLSLPAEWEWSRSRW
                     ERRFREENCGVLGGSDVAFLRYYATTALDLIMNPNHACAWLEIAEKDGFNQIIEQFWL
                     SACLDYHRFAADSPYHGVYARYIFPSHDAAFHPGLAARAGFTHLLGDAKQNAFVTKRL
                     EERVLREDPALYRQCVRVSQSHSTLAWSYV"
     gene            4715..>5240
                     /locus_tag="VFA02_13280"
     CDS             4715..>5240
                     /locus_tag="VFA02_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10869.1"
                     /translation="MKALSPLAKNQGTSLDRSRSSADKAGDKSFLPETRTFLSQASGT
                     LPNSITRIPLAPLGHPLMRKLAIGETNDPLEAEADRMAERVLRMTDPAHAASAPSHTA
                     PTLRRKCSCEGSGTKCKECEEAERGKKLHRKPQTTSAPAEAPPIVHDVLRSPGQPLDT
                     ATRAFFEPRFGADFS"
BASE COUNT         1132 a         1670 c         1318 g         1120 t
ORIGIN      
        1 gctacgcgtg aactactcct atggatttcc agcgccgctt ggcggcggcc cgtatgaccg
       61 ctccgccagt ctgccgttga cagacccttc aaagctgaat ttctttgccg tagccggatc
      121 agccgggaca cccacacttg agtctgccgt ggccctctgg aaccagcaac catccggcgc
      181 aacaggactt atcgttctgc ctaacttcga gtcgttcaac atagacctga ccggaacgaa
      241 cgcgatccgg cttcgggaag aaagccagct ttttattgtc gcggcagaaa cgcattctgc
      301 ttcaccgcag gagatcacct ttactaactc ctgcgcgacg cttacaggaa acatcgaagt
      361 gcaaagcacc gcagtcaatt ccacgcctgc cgggcaactc gtcttcagtg gcgtctggat
      421 agcaggtcag ctcaccatca ccgggcaggc agccaatgtt cagcttatgg actgcactct
      481 tgttcccggt cttgcactca gcacggatgg cgaaccgcag ttccctggcg aaccaagtat
      541 catcgtcagc gcaacagaag cggcgctctc gctcacccgg agcatctccg gccctattgc
      601 cgcggcgccg ggaggcacaa cgcgcatctg ctccagcgtt gtggacagca cttcgccctg
      661 ctgcattgcc tatgccggaa acgacctcgc ttctgccggc gccgatctgc atgtcgagga
      721 cagcaccatc atcggcaagg tacgcacacg cacgatgaca atggcatcag acactatctt
      781 ctacgcgcgt cttgcgcgcc gcgacccgtg gcccgcgccc gtctggtgca gccgccagca
      841 agccggatgt atgcgcttct gtttcgtgcc tgcgaattcc attacacctc gccgctacaa
      901 atgcctgcct gtggatgcag ccagcgaggc cgcgctcaga ccagacttca tcacgcggcg
      961 ctatggagac ccgtcatata tgctcctgag cggattcaac ccgctcgcca tctggcaagg
     1021 cgcggacaat ggctcgcaga tgggcgtgta caaccagttg caggaaaccg aggcggtccg
     1081 caacgtgcag ttgcgcgccc cggagtatct cccctttggt ctggaaagcg gaatctttct
     1141 cgtcccctcc cgtccgatcg ttgaacaacc tctacctcca actcatgcgt attacgggcc
     1201 gcagcgcgcc aggacctgtt gcgacgacga ggccgaggac cacggcttta caggaattgg
     1261 cgcaaatctg atttgaagca ggtgcacgtt atgaaaggcg acttcagccg catccggttt
     1321 tcccccgaga agcaatacac agccgtcatc gagcaacagg gacgtgtggc ccttgacgcc
     1381 gatgccaatg aacaaattgc gatcaacgag tatctctcgc gcgcagaaac cgtggacgtt
     1441 atcggcgaat acggcgcgcc ggccaacgat gctggcttcg ccatcagcgt gaacaacggc
     1501 gaaatcttca tcggcacggg ccgctattat gttgagggac tgatgtgtga aaacacaaac
     1561 agcaatctca cgctgagcac gcagcctttc ctcatagatc ccaacctgga tgaagccggg
     1621 ctcttacaag agttgcagcg ctacaaaggc gaggcatcta ttcgcgtcta tcttcaggtc
     1681 tggcaacgcc tcgtcaccgc gcttgacgac ccttgcctgc gcgaacctgc tctgggacaa
     1741 gccgatacga ccgcgcgtct ccagaccgtt tggcgcgttg ttgcatctct ggtggcccca
     1801 gccaatctga aacccggccc gattcgccaa cccatccctg gagaacggct gaacaggacc
     1861 aacccagtac tcagttccgt tgcggcacat cctgtcgccg gcattggaac ccatcccatc
     1921 acttccccgg tcagtgtctc cacaggcgtg tccggcacct cagggactgc accacttccc
     1981 atccatttgc tcacctgctg tgagcagatg taccttgacg ccaccgagct cagcaccgga
     2041 aaactcagcg cgcagaccag cggaacctcg gacacatgcg gctgccagcc cattcctgct
     2101 gccggttatc gagggcttga aaaccaactt taccgtgttg aaatccacac cggcggagat
     2161 gaaactaccg ctactttcaa gtggtcacgc gaaaatggtt ccgttgtttc tgccgtgctt
     2221 ggtgtgtccg gagcgaccgt ccaggtagac agtctcggcc ctgacgctaa tcttggtttc
     2281 caggccaacc agtgggtaga gatttacgac gacactttcc tcttcggccc tacgccaaat
     2341 cagcccggca atttgtatca gatacagagc attgatccag cctcaaactc catcaccatg
     2401 tacacgacag tcgctgcggt agaaacatgg cgaaacgcgc gcattcgccg gtgggaccag
     2461 accggcgcct cggccacttc aaacggtatt cctctctccg ccggaacatg gcttgatctc
     2521 gaaaacggta tccagatcag tttcgctgcc ggcacttatc aatctggcaa ttactggacc
     2581 atcccggcgc gcacagcctc tggccagatt gactggccac cctgcggcag cgatggatcg
     2641 ccctggcagc ttgcgaaatc cgttcgcgta tactcagcgc cgcttgcctg cattcactgg
     2701 gacataaaaa aagaagcgtt ttatgtggaa gattgccgcc gtctcttctc accattgacc
     2761 gaccttacag ctccagtcac tccgcaggcc atgcacgtta ctcaaatcag ttggacgaat
     2821 gatgacatca tcacactcga ccagcttgtt gccagcggtc tgacggttac gctggacggc
     2881 aacccctcag ggccagtcaa cagcgcaaat tttgttgttg atttcgaaat tgctgcccct
     2941 ccaccgaata cggattctgc gaaggagttt gctgctatct ttcagcgcgc catctttgaa
     3001 ggggcaccgt ccaccattct gcgtggactt accattactg attcgctcat tacagtgaac
     3061 aatggagcca ttctgtggca attgccttat ctcgaagcag gttacgaaca gcggatcacc
     3121 attctttatc tcaatgctct gctgagttat ggcgcgcgtg ccggatggtt cgcccgcgcg
     3181 cgtgtccgtc tgctgggccg caccatcttc tccggcagcg gaggaaacca gattttcctt
     3241 gatggccaga gttacggaca ggccgccctt cgcgccgacg ggaccacgcc gcgcattgat
     3301 ttgcaactgc cttcaggaaa tgatgaaaag gccagcgatt ttgaaagctg gttctacgtt
     3361 gcacccacac tcgttctttc atcactgact gtgaattacc cggcgctgac cgtggtcatc
     3421 aacgcaaaca atcaagtgac gggagtcact gccccgaacg cagcaggagc tccgcaacct
     3481 gtcgtgccgc aggcaacgct caccgtaaac tatccagcga ttgccgatac taccgtatct
     3541 ctcgcgctta ccggcacaag cggtgttgga accatcgcca gtgtcccatc cacagtaacc
     3601 atcaaccgaa atactacaag tgtgagcatt cccatttcag tgctcggcaa tccaggagca
     3661 aatacaacat acactttcca gatcacagca tccatcactt ctgcggttgg cctgacctct
     3721 cagttgactg catcttttac cgtgaccggc gtgccgccaa acatcattat tcagtgaaac
     3781 tcaagggagc aaagcgtatg cgcgctgtct ggtcgttctg gagtaagccg tttcaatccg
     3841 ccaaaggacg gacgtggaag acccacttcc atcacttgct ctcctgggga ctctcgttac
     3901 ggctcgcgcg cagacactat ccggaaaccg tgctcgtcac ggaccgtgca gggaaagcac
     3961 tgctcgtgga ccatctgggg ctctctttta cgcacgtctc gacggaactt gaacgcctgc
     4021 gccatgccga cccgtccttc tgggcatcag gcaagctggt tgcctacagc attcaggacc
     4081 aaccgtttct tcaccttgac acagacgttt ttctctgggc acggcttccc tcctctgtcg
     4141 aacagtcccc tatgttcgcg caatatccgg aacagcactc cacagatcag cactacagcc
     4201 cgcgcacggt cgagcgctta tttgagcgtc atcagctctc attgcctgct gagtgggagt
     4261 ggtcgcgctc gcggtgggaa cgccgtttcc gcgaagaaaa ctgcggcgtt cttggcggat
     4321 cagatgttgc ttttctgcgc tattacgcca caactgctct cgacctcatc atgaatccca
     4381 atcatgcctg cgcatggctg gagattgctg aaaaagatgg cttcaatcag atcatcgaac
     4441 agttctggct ctcggcttgc cttgattacc atcgcttcgc agcagactcg ccttatcatg
     4501 gagtctacgc gcgctatatc tttccgtctc acgatgcagc ttttcatccc ggactcgccg
     4561 cccgcgccgg attcactcac cttctcggcg atgccaagca aaatgctttt gtcacgaagc
     4621 gcctggaaga acgtgtactg cgcgaagatc ctgcgcttta tcgccagtgt gttcgggtaa
     4681 gtcagtctca ttccacactc gcatggagtt acgtatgaag gcgctttctc cattggcgaa
     4741 gaaccagggt acctcattgg accggtcacg cagttcagcc gacaaggccg gagacaaatc
     4801 attcctgcca gagacccgga cctttctgtc tcaggcttcc ggcacactcc ccaactccat
     4861 cacgcggatt ccgcttgcgc ccctcggtca tccgcttatg cgcaagctcg ccatcggaga
     4921 aaccaacgac ccgcttgaag cggaagcgga ccgcatggct gagcgcgttc tgcgcatgac
     4981 agaccctgcc catgcagcat ccgcaccatc acacacagca cccacgctcc ggcggaaatg
     5041 ttcctgcgaa ggttccggca caaaatgcaa agaatgcgaa gaggccgagc gcggaaaaaa
     5101 gctccatcgc aaacctcaga ccacatccgc gcctgcagaa gccccgccga ttgtgcatga
     5161 tgttttgcgc tctccaggac agccactcga tactgccacg cgtgccttct tcgagccacg
     5221 ctttggcgca gatttcagcc
//
LOCUS       DASRBV010000359        10464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_542383, whole genome shotgun sequence.
ACCESSION   DASRBV010000359 DASRBV010000000
VERSION     DASRBV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542383"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFA02_13285"
     CDS             complement(<1..1188)
                     /locus_tag="VFA02_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HZU10870.1"
                     /translation="MLDTQSRSRTVSRLAAVAAGLFAFAAGALAQNQPEAIVPNRITQ
                     PINSDVRVTLQHNVHPLAQARFDQGAAPEAMSTGRIMLILKHSPAQEIALKQFLSDAQ
                     NPASPNYRKWLTPAQYGQRFGVSDTDLNTVTAWLQSQGFTIEKVPQARNVVIFSGNVA
                     QVEQAFHTSIHKYVVNGEMHFANATDPQIPAALAPVVAGVGPLNDFRPKPTLVKGPRG
                     HYDPESKTIKPDLTLTNSSGQFFLYVDPADAATIYDTPNPTLNPNYSGTKYDGTGVTI
                     GIVGDSNVVMQDIINYRTAYLGESTNVNTPNVVVDGNDPGIISGDAVEALLDNEVAGG
                     IAPGAKINFYTAADNDFQSGLFLAIYRALDDNQVSILNVSFGACEAAQGVGGNAQILA
                     AWQQ"
     gene            complement(1941..3755)
                     /locus_tag="VFA02_13290"
     CDS             complement(1941..3755)
                     /locus_tag="VFA02_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU10871.1"
                     /translation="MATATTSASTSVKAPVGGSFLLEERQPGEIFTPEDFSDEQRQIA
                     ETAAQFAANEVLPAAEDIEAKHFDVTRALLKKAGDLGLMAVDVPEEYGGLGMDKVTSA
                     IIADRMSVLASFSVAFSAHVGIGTLPIVWYGTDAQKQKYLPKLATGEWIAAYALSEAS
                     SGSDAMNIRTRAVLSQDGKHYILNGEKMWITNAGFADLFTVFAKITDPNDSDPKNAKF
                     SAFLIERNTPGLTVGAEEHKLGIRGSSTCPLILSDCKVPAENLLGEAGKGHHIAFNIL
                     NIGRFKLGAACVGGARTSLANGIKYAKERKAFGKSIAEFGLIQQKIADCTARIFVGES
                     MAYRTVGMIDAALAAIPEEDAHNSREIQKRIEEYAVECSILKVWGSEMLDAVVDHVVQ
                     IYAGYGYVEEYPAERAYRDSRINRIFEGTNEINRLIITGFLMKRAMNGQLPLLAAIRQ
                     IMDEVMSPPAPNFDASGDDALGREAAILANAKKIALFCAGAASQKYMNALADQQEIMA
                     DLADIIMEAYALESALLRARKSQVALHSLATRHYAAHAMNVIQAAAGRVIAAVAEGDM
                     LRTQLAILRRLLKHEPTNTVESGRALAKMVIEAGRYPG"
     gene            complement(3823..4248)
                     /locus_tag="VFA02_13295"
     CDS             complement(3823..4248)
                     /locus_tag="VFA02_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU10872.1"
                     /translation="MSAYADTSLIVSIYLTDDHSRDADRIVMAHPGIFCTPLHLAEFA
                     HSIEQNVFRKRIYARSATRIYLEFERDRQAGIWNEIKLPELTFITAADLARSHVGTLG
                     VRTLDTLHVAAALELGAERFFTFDDRQKKLARAAGLKTA"
     gene            complement(4245..4487)
                     /locus_tag="VFA02_13300"
     CDS             complement(4245..4487)
                     /locus_tag="VFA02_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10873.1"
                     /translation="MITINMRQLRDTRKVKAWLKAGESLELWDRKQYIGKLLPPSKMV
                     PKKQIEWPDFAERRKRILGDRNLNAVENFLKERHRD"
     gene            complement(4537..5721)
                     /locus_tag="VFA02_13305"
     CDS             complement(4537..5721)
                     /locus_tag="VFA02_13305"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZU10874.1"
                     /translation="MRDAVIVSAVRTAVGKAPKGTLATTRPDDLAAVVIKGALEKVPA
                     LDPKEVEDVILGCAMPEAEQGMNVARIASLRAGLPVEASAMTINRFCSSGLQAIAIAA
                     DRIRSGSAEVMIAGGTESMSMVPMGGNKVSANPWLIEHYADSYLSMGLTAERVAAKYG
                     ITREQADAFSLSSHQKALAAIQEGKFAEEIVPVTVTYSSPGKNPAKPEVKTVEFTTDE
                     GPRADTSLEALAKLKPAFHAKGTVTAGNSSQTSDGAAAVVVMSAESARASGLKPLARF
                     VSFATAGCLPEEMGLGPVFAIPKALKLAGLTLDQIDLIELNEAFAAQSLAVVQQAGLD
                     PAKVNVNGGAIALGHPLGCTGAKLTATLLHEMKRRNLRYGLVTMCVGGGMGAAGIFES
                     LN"
     gene            complement(5730..6377)
                     /locus_tag="VFA02_13310"
     CDS             complement(5730..6377)
                     /locus_tag="VFA02_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10875.1"
                     /translation="MDQLFHCHPERSAASAKSKDLAALKCAAALLFLLCAVCVRAQTS
                     EQDITGQLQTPQGARSYRIRLLPLASFPSLPQNIVAQLDQRHCMVPQTFEAQHPENVV
                     RGSFEKKGSEDWAVLCAHEGTIDLLVFFKSGPQDPTTLATHKLTERMGAETPTSELGS
                     AWGISTIPPDGLRHTPGVHQHGPFDHDGIEDDFVERYSVVHYYRNGSWLTLEGNN"
     gene            complement(6380..8818)
                     /locus_tag="VFA02_13315"
     CDS             complement(6380..8818)
                     /locus_tag="VFA02_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU10876.1"
                     /translation="MTTANSSSSLLVRRCAVLGAGTMGSRIAAHLTNAAVPVLVLDVV
                     PEGADGQGRSRIAAQAIQALLKARPAAFYDPANAALIVPGNFEDDLPQLKSCDWIIEA
                     VTEDLGIKQALLEKIAPHLRPDAIVTTNTSGLPVGTIAEKMPLEFRRRWFGTHFFNPP
                     RYMRLLEVIRTPEADAQAIAAIADFADRRLGKAIVYARDTPNFIANRIGVFAMLTAIE
                     LMQQHDLTIEDVDALTGSAIGWPRTGTFRLADMVGIDVLAHVARNFSRMKAEDAAAAK
                     LPAFLETMLERKWLGDKTGQGFYKKIPDAPDPKNNRLALDWKTLEYRPAQRAKFPALE
                     MAKNAETLPERLRLLIGSGPGKDKGSIFQWTLLTRIWNYAADCWPEMADDLASIDRAM
                     RAGFNWELGPFEMWDAVGVPQSCEMLKSRNEHVSQQAELLLASGKASWYSDDAEFDLA
                     TREYRPVPAPEGIANVAAFRSTNGVIRKNAGASLVDIGEGIACIELHSKKNAIGDDIV
                     SLVTQTLLPDSEYVRNFRGFVISGDASDFSVGANLMQLLLAMQEGEWDDVDLAVRAFQ
                     RMTATVKFCARPVVVAPFSLCLGGGVEISLHGAARQPHAELYMGLVETGVGLIPAGGG
                     TKEMVLHAVDAAAKDSGLSPLDDPRRFAFSGELQEALKQRFETIAMAKVSTSAAEARK
                     LDLLLPSDKITLNRERLLLDARETAVYLAESGHAPPAPRMQVPVPGDQVGATLKLGVH
                     LMRQAEYISDYDAKIAHHLIRILCGGSVTPGSLVSEQYLLDLEREAFLSLCGERRTQE
                     RIAYTLKTGKPLRN"
     gene            8998..>10464
                     /locus_tag="VFA02_13320"
     CDS             8998..>10464
                     /locus_tag="VFA02_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZU10877.1"
                     /translation="MKFPSAVRLLIVLWCATLAAFAQDLGSFEKRVTTKVLPNGLTII
                     ICQRHDAPVFSYFTIVDAGSANDPDGESGLAHMFEHLAFKGTKDIGTTDYTAEKVALD
                     KVENAYAAYDAESLKRVGRDPEKLKQLHAAFEQALAEAQKYVIPNQFTEIAEENGAVG
                     LNAETSLDATQYFWSMPENRLQLWAYMESDRIANPVPREFYKERDVVMEERRMRVDSN
                     PIGRMVEQFLAAAYMAHPYRRPGVGWASELEHITATEAEAFHKKYYVPSDIVIAVVGD
                     IDPVQAMPVLEKYFGAIPAGPKPKPMTTVEPPQTAEKSVIIKEPTQPLYLEGYHKPDY
                     RDPDDAVYDAIQDIFSNGRTSRLYRSLVRDQQIAVEAEGFSGFPGDKYPGLFAFFAVP
                     APGHTPQEMQTAIHKEIDKLKTTDVSDDELAMFKTRARADILRGLADNQGLAEQLATY
                     QLRYGDWRELFNQLKRIDAVSKADIRRVANKIFVPTNRT"
BASE COUNT         2257 a         3087 c         2814 g         2306 t
ORIGIN      
        1 ttgctgccac gcggccagaa tctgagcatt cccaccgact ccctgcgcgg cctcacatgc
       61 gccaaagctg acattcaaaa tgctgacctg gttatcatct aacgcccggt aaattgcaag
      121 gaaaaggcca ctttgaaagt cattgtcagc agccgtataa aaattaattt ttgcgcccgg
      181 agcaatcccg cctgcaactt cgttgtccag caaagcctcg accgcatctc ccgaaataat
      241 gccaggatcg ttgccatcta cgactacatt gggagtattt acgttggtgg attcacccag
      301 atatgccgtg cggtaattga tgatgtcctg catgaccacg ttggagtccc cgacaatacc
      361 aatcgtcaca ccagtaccgt catactttgt cccgctgtag tttggattca gggttggatt
      421 tggcgtgtca tagattgtgg cagcatctgc tgggtctaca tacaggaaaa actgccctga
      481 ggagttggta agcgttaaat ccggcttgat ggttttgctt tccggatcat agtgccccct
      541 tggtcctttc accagcgttg gcttcggcct gaagtcattc agcggcccta ctcctgccac
      601 cacaggcgcc agcgccgctg gaatttgcgg atcggtggcg ttggcgaaat gcatctcgcc
      661 attcactacg tatttatgaa tggaggtatg gaaggcctgt tctacctgcg ccacgtttcc
      721 ggaaaaaatg accacattac gcgcctgggg taccttctct atcgtgaatc cctgagattg
      781 cagccatgcc gtgaccgtgt tcaggtccgt gtcgctgacg ccaaaacgct ggccatattg
      841 tgctggagtg agccacttgc ggtagttcgg cgatgccggg ttctgtgcgt cgctcaggaa
      901 ctgcttcagg gcaatctcct gcgcggggct atgcttcaga atcagcatga tgcggccggt
      961 ggacatcgcc tccggtgccg ccccctggtc aaatcgcgcc tgcgccagcg gatgcacgtt
     1021 gtgctgaagc gtaacgcgca catccgaatt gatgggctgt gtaatgcggt tcggaacaat
     1081 tgcctcgggc tggttctggg cgagggcccc ggcagcaaaa gcgaacagtc cggcggctac
     1141 tgcggccaga cgcgaaacag tgcgtgaacg tgattgcgta tccaacatgg ccatcctgta
     1201 aagctcggtg tggagtaggt tagggttgaa attggagact gtctgcacaa tgcacctgac
     1261 aacatggtgc tggaaagcgg atttccgtga cgcgctgaca agcgtggctc cgtgtgtcct
     1321 attagaccgc atttccaggg acaagttgcg cctctacggt cctttctttt tatatttgtc
     1381 tgctgcggca gatacaagcg aatgctccac gttagcagga tgactttgtg agctgtaaca
     1441 tttacacaaa gagagtctga ccatcccaca aaacgatagg tggcctcgaa accgggttct
     1501 tgcacgatgg ggtgaaaagc gcgtaccgga cttttccact ttggccccgt tgtcaagccc
     1561 tcatgaccgc tgtccttccg caaatctctg atttttaaag atcatcgtta taacaaggat
     1621 tgtccggttc atcctgtatc cagttgtata ctgaatatag tgcgagaagt tccggaaatt
     1681 ttcgtttccc cttgtgcctc ctcgcatcaa aggaaatact gtcaactaag ttgttgaaaa
     1741 taaacgattc ccgtaccgat caccacctgc aactcattga aaacaaagga ttcccgtaca
     1801 aagtacccgg ggggagggta tcacccccgc tgatcaggca aaccctgcgt ggatagttgc
     1861 accaaatcac aacttataac cctaatcttt gcaggaattt acaaaaaagt atggggcaga
     1921 cccaccgctc accccaccgc tcaccccgga tatctcccgg cttcaataac catctttgcc
     1981 agggcgcggc ccgactcaac tgtatttgtc ggctcatgct tcagtaggcg tcgcaggatg
     2041 gccagttgcg tccgaagcat gtcgccttcc gccacggcag cgataacgcg tcctgctgcc
     2101 gcctggataa cgttcatagc atgggcggcg tagtgccggg tggccagaga atgcagcgcg
     2161 acctgagact ttctcgcacg tagcaaggcc gactccagcg cataggcttc cataatgatg
     2221 tcggccagat cggccatgat ctcctgctga tcggccagcg cattcatgta cttctggctg
     2281 gccgccccgg cacagaagag cgcaatcttc tttgcattgg caagaattgc ggcttcgcgg
     2341 ccaagcgcgt catcgccgga tgcatcaaaa ttcggcgctg gcggagacat cacctcgtcc
     2401 atgatctgcc gaatggcagc aagcagcgga agctgcccgt tcatcgcccg cttcatcagg
     2461 aacccggtaa taatcagccg gttgatctcg ttcgtcccct caaaaatccg gttgatgcgc
     2521 gaatcgcgat aggcccgttc agccggatac tcctccacat acccataccc ggcataaatc
     2581 tggacaacgt ggtccaccac tgcgtcgagc atctcagagc cccagacctt caggatggag
     2641 cactcaacgg cgtactcctc aatccgcttt tgaatttcac gggagttatg cgcgtcttct
     2701 tcagggatgg ccgccagcgc agcatcaatc atgccgacgg tgcgataagc catgctctcg
     2761 cccacaaaga tgcgcgccgt gcagtcggca atcttctgct ggataagccc aaactcggcg
     2821 atggacttgc cgaatgcctt gcgctctttc gcgtatttga ttccgttggc cagcgatgtg
     2881 cgcgcgccgc ccacgcacgc cgctcccagc ttgaagcgcc caatattcag aatattgaaa
     2941 gcaatgtggt ggcccttccc tgcctcgccc agaaggtttt cagcaggcac cttgcaatcc
     3001 gaaagaatca gcgggcaggt agacgagcca cgaatgccca gcttgtgctc ctctgccccg
     3061 accgtcagcc cgggcgtgtt gcgctcaatc aggaaggccg agaactttgc atttttcggg
     3121 tcggaatcat ttgggtccgt gattttcgca aagacggtga acagatcggc gaaacctgcg
     3181 ttggtgatcc acatcttttc gccgttcaga atgtagtgtt tgccgtcttg agaaagcacg
     3241 gcgcgcgtgc gaatgttcat cgcgtcggag ccggacgagg cctcagaaag cgcataggcg
     3301 gcaatccact cgcctgtggc cagcttcggc agatattttt gcttttgcgc atcggtccca
     3361 taccaaacga tgggcaatgt gccgatcccc acatgcgcac tgaaggccac cgaaaagctg
     3421 gctagcaccg acatgcggtc ggcgatgatg gccgacgtga ccttgtccat accaaggccg
     3481 ccgtactctt caggaacatc tacagccatc agccccaggt ctccggcctt tttcagtagc
     3541 gcgcgcgtta cgtcaaagtg cttggcttcg atgtcttcag cggcaggcag cacttcgttc
     3601 gcagcaaatt gcgcggcggt ttcagcaatc tggcgctgct cgtcagaaaa atcttccggc
     3661 gtaaaaattt caccaggctg gcgttcttct agaagaaagc tgcctccgac aggggccttg
     3721 acagaagtgg aagcggaagt ggtggctgtg gccatgagag atgctcctgt tttgctcaac
     3781 gaagcatctt acgcctcttt gatgaaaatt tgtgagatgg ggtcaggcag tcttcagacc
     3841 agcggcccgg gccagcttct tctggcgatc atcgaaagtg aaaaagcgct cagcaccaag
     3901 ttcgagggcc gccgcaacgt gcagcgtgtc gagtgtgcga acacccaatg tgccaacatg
     3961 agatcgcgca agatccgcag ctgtgataaa cgtcagctct ggcagtttga tctcattcca
     4021 tatgccagcc tggcggtcac gttcaaattc aagatagatc cgagtggcac tgcgagcata
     4081 aattcttttg cgaaagacat tctgctctat ggaatgcgca aattccgcca gatgcaacgg
     4141 agtacagaaa attcccggat gcgccatgac aatccgatca gcatctcgcg aatggtcatc
     4201 cgtcagataa atggagacta tgaggcttgt atcggcgtat gcgctcaatc ccggtggcgt
     4261 tccttcagga aattttctac ggcattcaga ttacggtcgc caaggattct cttccggcgc
     4321 tctgcaaaat caggccattc aatttgcttt ttggggacca tttttgaagg cggcaaaagc
     4381 ttgcctatgt actgttttct gtcccatagt tccaaagatt cgcctgcctt cagccacgcc
     4441 ttgactttgc gggtatcgcg aagttgccgc atattaatcg taatcatgaa taatattgtg
     4501 acacattttg tgtcacattg cgaatatacc gtactttcaa ttcaaactct cgaatatccc
     4561 ggctgctccc atgcctccgc cgacgcacat ggtgaccagc ccatagcgca gatttcgccg
     4621 cttcatctca tgcaaaaggg tcgcggtcag cttcgcgccg gtgcagccca gcggatgccc
     4681 cagcgcaatc gcgccaccgt ttacgttgac ttttgcagga tcaagcccgg cctgctggac
     4741 cacggccagg gactgcgcgg cgaaggcctc gttcagctca atcagatcta tctggtccag
     4801 tgtgagtccc gcgagtttca gggccttcgg aatggcaaac accgggccga gccccatctc
     4861 ttccgggagg catccggcag tagcaaagct gacaaagcgc gccagcggtt tcagccccga
     4921 tgcccgcgcg ctctcagcgg acatgacgac aactgcggcc gctccgtctg aggtctgcga
     4981 tgagtttcct gctgtcactg tgcctttcgc atgaaaggca ggcttaagtt ttgccagcgc
     5041 ttcaagcgag gtgtccgcac ggggaccttc atccgttgtg aactcaactg ttttcacttc
     5101 cggttttgcc ggattctttc ccggtgaaga atatgtaacc gtaacaggca caatttcctc
     5161 tgcaaatttt ccttcctgaa ttgcagccaa cgccttctga tggctactca gagaaaacgc
     5221 atcggcctgt tcgcgcgtga tgccgtattt tgctgccact cgttcggcag tcaggcccat
     5281 ggagagatag ctgtcggcgt agtgctcgat gagccacgga ttcgcactca ccttgttgcc
     5341 gcccatgggc accatgctca tggattcagt ccctccggca atcatcacct cagccgagcc
     5401 ggagcggatg cggtcggcag caatagcaat ggcctgaagc ccagaagagc agaagcgatt
     5461 gatggtcatg gccgatgcct caaccggcaa cccggcgcgc agactggcaa tacgggccac
     5521 attcattccc tgctctgctt cgggcatggc gcaacccaga attacgtctt ctacttcttt
     5581 tggatccagc gcagggacct tttcgagcgc acccttgatg acgactgccg ccagatcatc
     5641 aggacgcgta gtggccagcg ttcctttcgg cgctttgccc accgccgtgc gaacagcgct
     5701 tacaattacc gcatctctca tgatgcacct cagttgtttc cttcaagcgt tagccagctt
     5761 ccattcctgt aatagtggac gacggaatag cgctctacaa aatcatcctc aatcccgtca
     5821 tgatcaaagg ggccatgctg atgcactcct ggggtatgcc gcaacccgtc aggtggaatt
     5881 gtggagattc cccatgctga tccaagctcg cttgtaggcg tctccgcgcc catgcgttca
     5941 gtcagtttgt gtgttgccag cgtcgtggga tcctgcggac cgctcttaaa aaacaccagc
     6001 aggtcaattg ttccttcatg cgcacaaagc acagcccagt cttcgctgcc cttcttctca
     6061 aaagacccgc ggaccacatt ttcagggtgc tgtgcctcaa atgtctgcgg gaccatgcag
     6121 tggcgctggt cgagttgggc cacaatgttt tgcggcagcg atggaaatga ggccagcggc
     6181 aggaggcgta tgcgatagct tcgcgctccc tgcggagttt gcagttgacc tgtgatgtcc
     6241 tgttctgagg tttgtgcgcg aacgcatacg gcgcacagca gaaacagcag tgcggcggcg
     6301 catttaaggg cagccaggtc cttcgacttc gcgctcgcgg cgcttcgctc aggatgacaa
     6361 tggaagagtt ggtccatttt taattccgca gcggctttcc cgtcttcagc gtgtaagcga
     6421 tgcgctcctg cgttctacgc tctccgcaga gggaaagaaa tgcctcacgt tccaggtcca
     6481 acagatattg ctcgctgacc aggcttcccg gagtcacgct tcccccgcac aggatgcgta
     6541 tcaggtggtg tgcgatctta gcatcgtaat cgctgatgta ctcggcctgc cgcatcaggt
     6601 ggacgcccag cttcagcgtt gcgcctactt ggtcacctgg aacagggacc tgcatacgcg
     6661 gtgctggtgg cgcatggcct gattcggcca gatacacagc cgtctcgcgc gcatccagca
     6721 acaagcgctc gcggttcaga gtgattttgt cggatggaag cagcaggtct agtttgcgcg
     6781 cctcagcggc cgaggtcgag accttcgcca tcgcaatcgt ctcaaagcgt tgcttcagcg
     6841 cctcctgaag ttcaccggag aaggcgaacc tgcgcgggtc atccagcggg ctaagaccgg
     6901 agtcctttgc tgccgcgtcc actgcatgga gtaccatctc ctttgtgccc ccgccagcgg
     6961 gaataaggcc gaccccggtt tcgaccaggc ccatatacag ctcggcatga ggttgacgcg
     7021 cggccccgtg cagtgaaatc tccactccgc cgccaagaca cagactgaac ggcgccacca
     7081 cgaccggacg cgcgcagaac ttcaccgtcg ccgtcatgcg ctgaaaggcg cggaccgcga
     7141 ggtctacgtc atcccattca ccttcctgca tggccagcag caactgcatc aggttcgcgc
     7201 cgacagaaaa atccgaagca tctccgctga tgacgaaccc gcgaaagttg cgtacatact
     7261 cgctgtccgg aagcagcgtc tgcgtcacca gcgagacaat gtcgtcaccg attgcatttt
     7321 tctttgagtg caactcgatg caggcaatgc cttcgccaat gtccaccagc gaagcgcctg
     7381 catttttgcg gatgacgccg ttggtgctcc tgaaagcagc aacgttggca atgccttccg
     7441 gcgcggggac cggcctgtac tcgcgcgtgg ccagatcaaa ttcagcgtcg tcgctgtacc
     7501 agcttgcttt gccagaagcc agcagcagtt ctgcctgctg gctcacatgc tcgttgcgcg
     7561 atttgagcat ttcgcaggac tgcggcacgc ccacggcgtc ccacatctca aacgggccaa
     7621 gctcccagtt gaacccggca cgcatggcgc ggtctatcga ggcgaggtca tccgccattt
     7681 ccggccagca gtcggcggca tagttccaga tacgcgtcaa aagcgtccat tggaagatgc
     7741 tgcccttgtc cttgcccggg ccggagccaa tcagcagacg caggcgttcg ggcaatgtct
     7801 ccgcgttctt tgccatttcg agcgcgggaa atttcgccct ctgcgcagga cggtactcca
     7861 gcgtcttcca gtcgagggcg agccggttgt tcttcgggtc tggcgcgtcg ggaatcttct
     7921 tatagaagcc ctggcctgtt ttgtcgccca gccattttcg ttccagcatg gtttcaagaa
     7981 acgcgggcag ctttgcggct gcggcatctt ctgctttcat gcgggaaaag ttcctcgcta
     8041 catgcgccag cacatcaata ccaaccatat ctgccaggcg aaacgtgcct gtgcgaggcc
     8101 agccaatcgc agagccagtc agcgcgtcca catcctcaat cgttagatcg tgctgctgca
     8161 tcagctcaat cgcagtcagc atggcgaaca cgccgatgcg gttagcaatg aaatttggcg
     8221 tgtcacgagc gtaaacaatg gctttgccca gcctgcggtc ggcaaaatca gcaatcgcag
     8281 caatggcctg cgcatcggct tccggagtgc ggatgacctc aagcagccgc atatatcgcg
     8341 gcggattgaa gaagtgcgtc ccgaaccagc gccgccggaa ctccagcggc atcttttccg
     8401 caatcgtacc cacaggaagc ccgctggtgt tagtagtcac aatcgcatct gggcgcagat
     8461 ggggcgcaat tttttcaagc agcgcctgtt taattccgag gtcttcagta acggcctcaa
     8521 tgatccagtc gcaggatttg agttgcggca gatcgtcttc aaagtttcca ggcacaatca
     8581 gggccgcatt cgcagggtca taaaatgctg ctggcctggc cttgagcagc gcctgtatgg
     8641 cctgcgcggc aatccggctg cggccttgcc cgtccgcgcc ctcaggtacg acgtccagca
     8701 ccagcaccgg gaccgcggca ttcgttaaat gtgcggcgat gcgggaaccc atcgtgcctg
     8761 ctccaagcac ggcacagcgg cggaccagca gggaggagga agaattagca gtggtcattg
     8821 aggacgctcc gggatacggc gcagaatact aagctgttcc gcggtgggtc aagtttgaca
     8881 caccatgaga cgacaggtag cgtcaaaata gttttgggac ggattttctg tttcaattca
     8941 ttttgacacc ctgagcgaag ccgagggacc tgcttccatc aggacatcag aggccgcatg
     9001 aaatttcctt ctgctgtgcg tttgcttatc gtgctctggt gcgcgacgct ggccgctttt
     9061 gcccaggacc tgggcagctt tgaaaagcgc gtcaccacca aagttctgcc gaatgggctg
     9121 accatcatca tctgccagcg ccacgacgct ccggtcttct cctacttcac tatcgtagac
     9181 gcgggcagcg ccaatgaccc cgatggggag tcgggcctgg cgcacatgtt cgaacacctg
     9241 gccttcaagg gcacaaagga catcggcacc acggattaca ccgcggaaaa agttgcgctc
     9301 gacaaagttg agaatgccta tgccgcatat gatgccgagt ctctcaagcg tgtggggcgc
     9361 gacccggaaa agctgaagca acttcacgct gcatttgagc aggcgctggc cgaggcgcag
     9421 aaatatgtca tccccaacca gttcacggaa attgcagaag aaaacggtgc tgtcggcctc
     9481 aacgccgaaa ccagcctgga cgccacgcaa tacttctgga gcatgccaga aaaccgcctc
     9541 cagctctggg cgtatatgga aagcgaccgc atcgccaacc ctgttccgcg cgaattttac
     9601 aaagagcgcg acgtggtcat ggaagagcgc cgcatgaggg tggactcaaa ccccatcggc
     9661 cgcatggtcg agcagtttct tgcagccgcc tatatggcgc acccctaccg gcgtccagga
     9721 gtaggctggg ccagcgaact tgagcacatc accgcgaccg aggccgaggc cttccacaaa
     9781 aaatactatg tgccgtcgga cattgtgatt gccgtggttg gcgacataga ccccgtccag
     9841 gccatgcccg tcctggaaaa atatttcggc gcgattcccg cgggcccaaa gccgaagccg
     9901 atgacaacgg ttgagcctcc ccaaacggcc gagaagtccg tcattatcaa agaaccaacg
     9961 cagccattgt atcttgaggg ttatcacaaa cccgactacc gcgatcctga tgatgccgtc
    10021 tatgacgcga ttcaggacat cttctccaac ggccgcactt cgcgtctcta ccgcagcctg
    10081 gtccgtgacc agcagattgc agtggaggcc gaaggcttca gcggctttcc cggcgacaaa
    10141 taccccggtc ttttcgcctt cttcgcagtg cccgctcccg gccacacccc ccaggagatg
    10201 cagactgcca ttcacaaaga aattgacaag ctgaagacta ccgacgtttc cgacgacgaa
    10261 ttggccatgt tcaagacacg cgcccgtgcc gacatcctgc ggggcctggc cgataatcag
    10321 ggtctggccg aacagcttgc cacctaccag ttgcgctacg gagactggcg cgaactcttc
    10381 aatcagctca aacggattga tgcagtcagc aaggcagaca tccgccgcgt cgccaacaaa
    10441 atttttgttc caaccaacag aaca
//
LOCUS       DASRBV010000360         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_281639, whole genome shotgun sequence.
ACCESSION   DASRBV010000360 DASRBV010000000
VERSION     DASRBV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281639"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFA02_13325"
     CDS             complement(<1..1025)
                     /locus_tag="VFA02_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU10878.1"
                     /translation="MPITEVPTSPAGAPPEPPRNRRTQQYEHLERHELLQVMDELQDE
                     RARARLREVFWVSIIIHMVVFWFLLYGPKYVFHQVKVRDPFSVLKEREKQLTYLDLPP
                     DLQKPVKPKRSDIISDKDRVAQSRRPTLDRKTLEELEAMKRAGPPAPQPAEKPGQTPQ
                     PPAPQQQAQAAPPPPQPMPQQRQTPSQPLRQNEQAQLEAPAPKPAPTQPNFNTGGMTA
                     GQAIQQAARNAIRSGQVGGGGDMGANAPSAHPGVNGAVDILSDTMGVDFGPYIRRVIY
                     DTERSWWPIIPESARPPISKQGKVGIRFKILPDGSVKEMVLELPSGDVALDRAAWGGI
                     TGASPYPP"
     gene            1166..1663
                     /locus_tag="VFA02_13330"
     CDS             1166..1663
                     /locus_tag="VFA02_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZU10879.1"
                     /translation="MPTAAVITVSDSCFEGTRQDLSGPAVTELLTANGFTVVRQLIVA
                     DVRSSIEDALRTCCDEAALVITTGGTGVAPRDVTPEATRNVCERLLDGMAEVMRAEGR
                     RETPYAALSRAICGTRGQSLIVNLPGSPRGATTSLRAVLPLLAHALGLLAGTETHQTS
                     NAGNR"
     gene            1669..2301
                     /locus_tag="VFA02_13335"
     CDS             1669..2301
                     /locus_tag="VFA02_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZU10880.1"
                     /translation="MKHLSALFLKFSAWLVAVLKPLGFWGAGVLALLDSSTIPVPMDL
                     IISGYVWADHRHFYLYVIMAAAGSSVGGLLPFLLGRAGGELFLLKRVDRARYEQMRDQ
                     FERQEFVAVLVPSMLPPPTPWKLFVFGAGVFEMKLRDFLLAVFLGRLIRFTVEALLVI
                     RYGPQIVTLIHDLARQHLSALLIGIGVVFAVLIVFALRKGIPGTRKKTDN"
     gene            complement(2390..>2672)
                     /locus_tag="VFA02_13340"
     CDS             complement(2390..>2672)
                     /locus_tag="VFA02_13340"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZU10881.1"
                     /translation="LELPLDVRATAFQRRVWEALRRIPRGQTRTYSQIAKEIGQPTAV
                     RAVARACATNPVAVVVPCHRVIGSDGTLTGYRWGLERKKKLLELEQTAS"
BASE COUNT          469 a          782 c          836 g          585 t
ORIGIN      
        1 ggcggatagg gtgaagctcc ggtgataccg ccccaggcgg cgcggtcgag cgccacgtct
       61 ccggaaggaa gttcgaggac catctccttc acgctgccgt cgggcagaat cttaaagcgg
      121 atgccgactt tgccctgttt ggagataggt ggacgcgccg actccggaat gatgggccac
      181 catgagcgct cggtgtcgta aatcacgcgg cggatgtagg ggccgaagtc tacgcccatc
      241 gtgtcagaga gaatgtctac agcgccattg actcccggat gggccgaggg agcatttgcg
      301 cccatgtcgc cgcccccgcc aacctggccg gagcggatgg cgttccgcgc cgcctgctgg
      361 atggcctgac ctgcggtcat tcctccggtg ttgaagttgg gttgcgttgg cgccggtttg
      421 ggcgcgggag cttccagttg cgcctgttca ttctgccgta aaggctgtga cggcgtctgc
      481 cgctgctgcg gcatcggctg tggtggtgga ggagcagcct gggcctgctg ttgcggcgcc
      541 ggaggctgcg gcgtctgacc cggcttttcc gccggttgcg gagcaggcgg tccggcgcgc
      601 ttcattgcct caagctcttc gagggtcttg cggtcgagcg taggacgtct ggactgcgca
      661 acccggtctt tgtctgaaat aatgtctgac cgctttggct tgactggttt ttgcaggtcc
      721 ggcggcaggt ccaggtaggt cagttgcttt tcccgttctt tcagaacaga aaacgggtcg
      781 cgcaccttga cctgatgaaa aacgtatttt ggcccatata agaggaacca gaacaccacc
      841 atgtggatga tgatggagac ccagaagacc tcacgcagac gcgcacgcgc gcgctcgtcc
      901 tgcaactcat ccatcacctg aagcagctcg tggcgctcaa ggtgctcgta ttgctgagtt
      961 cggcgattac gcggcggctc gggcggagcg ccagcggggc tggttggaac ttctgtgatg
     1021 ggcattcgga aacggtttca ggctgccggc cggattttcc tgtatcctct tattctctca
     1081 tacagagacg aaaatcctcc ctgttggact catccttcgg tagaaacggt agttcctctg
     1141 cttagtccca ttacaatcaa aaggtatgcc aacggcagcc gtgattaccg tcagcgattc
     1201 ctgttttgag ggcacgcggc aggacctgtc cggccctgcc gtgacggaac tgctgacggc
     1261 caatggattt acagtcgtcc ggcaactcat cgtcgcggac gtgcgatctt ccatagaaga
     1321 cgcattgcgg acgtgctgcg acgaagcggc actggtgatc accaccggag gaacaggggt
     1381 ggccccacgg gacgttacgc cggaggcaac gcgcaatgtg tgcgagcgcc tgctggacgg
     1441 catggccgag gtgatgcgcg ccgagggccg cagggaaacg ccttacgccg cgctgagccg
     1501 cgcaatttgc ggaacacgcg gccagtcgct aattgtcaat ctgccgggca gcccgcgcgg
     1561 agcgacaacg tcattgcgcg ctgttttacc gctgcttgcg catgcgctcg gtctgctcgc
     1621 cgggacggaa acacatcaga cctccaacgc aggaaacaga taaccgtctt gaaacacctt
     1681 tccgcccttt ttctgaagtt cagcgcatgg cttgtggccg tgctgaagcc actgggcttc
     1741 tggggagcgg gcgtgctcgc gctgcttgat tcgagcacca tccccgtgcc catggacctc
     1801 atcatttccg gatatgtctg ggccgaccac aggcactttt atctttatgt gatcatggcc
     1861 gccgccggct cttcggttgg cggcctgctg ccgtttcttc ttggccgcgc cgggggcgaa
     1921 ctctttctgc tgaagcgcgt agaccgcgcc cgctacgaac agatgcgcga ccagtttgaa
     1981 cggcaggagt ttgtcgctgt ccttgtgccc tccatgctgc ctccgcccac gccgtggaag
     2041 ttatttgtct ttggcgcagg cgtttttgaa atgaagctgc gagactttct gctggccgtt
     2101 tttctgggac gcctcatccg gtttacagtc gaagcgctgc tcgtcatccg ctacggcccg
     2161 cagattgtca cgctgattca cgatctcgca aggcagcatc tgagcgcgct gctgattggc
     2221 attggcgtag tcttcgccgt gctgatcgtc tttgcgctgc ggaaggggat tcccgggacc
     2281 aggaagaaaa cggacaacta gattggctct atattggctc tatcctgaat cgctgattct
     2341 tcctcccttc ggtcgtagaa tgacggactg tacgtatacg aaaaatgctc taagaagcgg
     2401 tctgctccag ttccagcagt tttttcttcc gttccaggcc ccagcggtat ccggtgagcg
     2461 tgccgtcgct gccgatgacg cgatggcaag gaacgacaac ggcgaccgga ttcgtagcac
     2521 aggcacgtgc cactgcgcga acagccgtgg gctggccgat ttccttcgca atctgcgagt
     2581 aggtgcgtgt ctggccgcga ggaatgcggc gcagcgcctc ccacacacgg cgctggaagg
     2641 ccgtcgcgcg gacgtcgagc ggcagctcga ga
//
LOCUS       DASRBV010000361         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_637091, whole genome shotgun sequence.
ACCESSION   DASRBV010000361 DASRBV010000000
VERSION     DASRBV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637091"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="bshC"
                     /locus_tag="VFA02_13345"
     CDS             complement(<1..627)
                     /gene="bshC"
                     /locus_tag="VFA02_13345"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HZU10882.1"
                     /translation="MHVDCFPITVLPHLSRLFTDYAQSHTPLRFYRQSPYSREWETQP
                     SLMTAERRSAIADLLSAQNRAFGAGDRTFANIERLRNGANAVVTGQQVTLFGGPLYTL
                     LKAATAIRKAEDSGCVPIFWMATEDHDLAEADHVALPTRHELRTFRLELENYGASVGA
                     LKLGKGIRPVLDDAAEILGGSEVFEILEGCYTPDATFAQAFGCLLSRIF"
     gene            complement(788..1627)
                     /locus_tag="VFA02_13350"
     CDS             complement(788..1627)
                     /locus_tag="VFA02_13350"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HZU10883.1"
                     /translation="MAKKLDIAIIGLGNWGTSLAYGCLSARVPLKEVAVRALPEARSR
                     LPVVRLEDAKLDARVLWICVRDAEIGDVAEQIAAQRGSLEGQIVVHSSGALSATVLEA
                     AKRAGAQVGSIAPVMSFPTRAPVALNGVMFAIEAEKNIRTILELLVRTLGGDPFFIEA
                     EKKPLYHAAATMASPLLLAELAAAVAAARSAGLPEKLAAKWVGALAQTTLKNFMARGA
                     EKSFSGPFARGDVSTIRLHLQVLGEHPMLKEIYGQLARYAVEELPVQNREELSALLRK
                     VEK"
     gene            1673..2257
                     /locus_tag="VFA02_13355"
     CDS             1673..2257
                     /locus_tag="VFA02_13355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZU10884.1"
                     /translation="MPSKPVLSAKKADIEVLSSRTAFQGPLFRVDVDKVREPGNRISR
                     RDVIRHNGSVVILAVDDSSKKDPLIVMERQYRHAAGQYMWEIPAGKIEEGEDRLAAAK
                     RELREETGYIAKKWTKLTRYIPSPGFLGEWMQVFLAEGLTAGDTSPDEDEWLEVALAP
                     LSEILSKIEAGKILDGKTLIAVLLFTHLRAKRKR"
     gene            2421..2639
                     /locus_tag="VFA02_13360"
     CDS             2421..2639
                     /locus_tag="VFA02_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZU10885.1"
                     /translation="MSTYKGKVKWFNNAKGYGFIGRDDGPDVFVHYSAIQLDGYKSLK
                     EGDEVEFDIVQGQKGPQADQVIRTREVA"
     gene            2765..4516
                     /gene="polX"
                     /locus_tag="VFA02_13365"
     CDS             2765..4516
                     /gene="polX"
                     /locus_tag="VFA02_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZU10886.1"
                     /translation="MDNRSIAQLLYETADLLEISAGDPFRIRSYRRAAEAVESSTIQL
                     SQIAHDPKKLQEIPGIGKGMAANIQEIEKTGTLPLREELLTKYRPGMLDLLRLPGMGP
                     KSVALFWEALQISSIAELEAAIAAGKLGSLPRMGTKQVEKLRKGIEDYKKNSGRFLID
                     DAEIAAEKLIAYLRNFDGMDVITPAGSLRRGRETVGDLDILVTGRACAEDKVQAAVDY
                     TAAYPPIVDLIAKGQNKVSFRLRGGLQVDVRLLPENSYGAALQYFTGSKMHNVSIRQR
                     ALKRGYTLSEYSLARVEDGSFVAGATEEEIYAALGLDWIPPELRENNGEIEAAEQHRL
                     PTLIELKDIRGDVHMHTNATDGKCSIREMAEAAVQRGYEYIAITDHTKNLAMTFGLDD
                     ERALEHIRRIREVNDEMSGRIRIFAGMEVDILADGQLDLADETLAQLDVVIVSVHSLF
                     NMTEEEMTARILRAIENPYTRILGHATGRLLLRREAYKLNLPQILRRAAELGVAVEHN
                     AYPDRLDLCDRDLRLAKELGCRIVINTDSHHTSHMEKMRYGISQLRRAWLTKNDVLNT
                     LPPDEFLTALRASPAPK"
     gene            4513..5649
                     /locus_tag="VFA02_13370"
     CDS             4513..5649
                     /locus_tag="VFA02_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZU10887.1"
                     /translation="MKLAPFRSTRYSHLVGFQDFLGNETAVRHLREGLAHGRLPHALI
                     LAGPRGSGKYTLALMLAQAANCLNPQQTGGLPDFCGECRNCTRIAESMPLETRVEEAI
                     AAREELREADKKETRILVQTHPDVLVVPPDPPQLLIKLGQVRTVIREIYRIPSEAKRA
                     FYIFTSAAFMKEAANSLLKVLEEPPAHASIFLLAENTGELLPTIRSRAGIVRLGALPF
                     DQLEQLIARRRSDLRPAQRSLVARLAQGAVGAALGFNIEQYLAHRQDALILLRYATRE
                     SDHSTLFRMTETYRAGAEGQEKTEGMLRALSTLLEDLLLLQNGQPGLVRNTDIQGELA
                     SIAGTVSFEWIESTARAIQQVRSGMRRNLLRSLALDAFAAEVHV"
     gene            5845..6141
                     /locus_tag="VFA02_13375"
     CDS             5845..6141
                     /locus_tag="VFA02_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZU10888.1"
                     /translation="MPADFISNLYVFILAGFVGFELIKRVSPLLHTPLMSLTNALDAI
                     VVVAAIIITGRHGSPLTTTLGAIAVAASFSNMVGGFFITDRMVRMFKSGGSRKA"
     gene            6138..7532
                     /locus_tag="VFA02_13380"
     CDS             6138..7532
                     /locus_tag="VFA02_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZU10889.1"
                     /translation="MNAAAIQQYLDFAYIAAIVLFMLALKWLSSPVSARRGVLAGEIA
                     AGLAVLATLNNPEVVTYKWIIIALILGAGIGIPLGMVKITAVPQRTALSHAFGALSAA
                     MIGIAEYYEGLDHLTRFGMGEIALEVIIGSLTFTGSLIAAGKLQEILPQRPIVYKGQN
                     IISLSVLAAALGLAVALVIHPQQTTLFVAMVVVALVFGIMLVTPIGGADMPTVISLLN
                     SYAGFSAALLGFVLNSKMLIVAGALDGSSGFILSVIMCRAMNRSFTNVLFGAFGTVQT
                     TVTKTEARPIRSASAEEAAQILDAARKVVIIPGYGMAVAQAQHKLRELYDVLTKRGVE
                     VSFAIHPVAGRMPGHMNVLLAEADVPYDRLIEMDEINPEFPRTDVALVIGANDVVNPA
                     ARFTKGSPISGMPILNAYEARTVMVIKRSMNPGFAGIDNDLYYLDKTLMLFGDAKAFV
                     GSLIKELNAVAAGV"
     gene            7682..9022
                     /locus_tag="VFA02_13385"
     CDS             7682..9022
                     /locus_tag="VFA02_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZU10890.1"
                     /translation="MGLFSNAPGLSTTPATAPLAERMRPRTLDEYVGQEHLLGPGKPL
                     RVQIERDDASSMIFWGPPGVGKTTLAKIIAETTRASFIEYSAVLSGIKEIKQVMADAE
                     KAATYGSRTILFVDEIHRFNKAQQDAFLPYVERGSIRLIGATTENPSFEIISALLSRC
                     RVYTLRALTDRQILTLIERALSDKERGLGNLNVTADHEALELLANYANGDARRALNAL
                     EVSAGLAQVSGGRISTVTATEAIQQRVLLYDKQGEEHYNLISALHKSVRNSDADAALY
                     WLGRMLESGEDPMYVARRLVRMAVEDIGLAAPEALNLCLSARDAMDFLGSPEGELALA
                     QAAVYLALAPKSNALYTAYGQVLSDIEHTRAEPVPLHLRNAPTGFMKSLGYSKGYKYA
                     HDEAGRVADMECLPESLRGRTYYHPTNEGREKLLAQRMEEVRKIKEAKKSEKEK"
BASE COUNT         2030 a         2976 c         2248 g         1818 t
ORIGIN      
        1 aaagatgcgg gacaacaaac agccaaaggc ctgcgcaaag gtggcgtccg gtgtgtaaca
       61 gccttccaga atttcaaaga cctcgctgcc cccgagtatc tcagccgcat cgtccagcac
      121 cggacgaatg cctttgccca gctttagcgc gccaactgag gcgccgtaat tttccagttc
      181 cagcctgaag gtgcgaagct catggcgggt cggcaacgca acatgatcgg cctccgcaag
      241 atcatggtct tcggtcgcca tccagaagat gggcacacag cccgagtcct cggccttacg
      301 aatagcggta gcggccttca gcagcgtata caaaggtccg ccaaacagcg tcacttgctg
      361 tcctgtaaca actgcattgg ctccattgcg cagccgctcg atatttgcaa acgtcctgtc
      421 gcccgctccg aatgcccggt tctgcgctga aagcaaatcc gcaatggcag accgccgttc
      481 cgcagtcatc agggatggct gcgtttccca ttcacgcgaa tatggactct gccggtaaaa
      541 acgcaatggt gtgtggctct gcgcgtagtc agtgaacagg cgcgagaggt gcggcaaaac
      601 cgtaattgga aaacagtcta cgtgcaattt tttcgttctc cagcgcggga tggtgtattc
      661 agtgtctcac gtgaaaccgg aatcccggac aatctcactt gcaacccttt gcggctctcc
      721 gcggttaaaa atccatgccg aaatcgcata gctgacccgg gccttgtgga tgcagggaat
      781 gaaaccttca cttctcaacc ttcctcagca aggcacttaa ctcctcccgg ttctgcacag
      841 gcaactcttc caccgcatac cgtgcaagct ggccataaat ctctttgagc atcggatgct
      901 cgcccagtac ctgaagatgc agtcttattg tggacacgtc ccctcgcgca aatggcccgc
      961 tgaagctctt ctctgctccg cgggccataa aatttttcag tgttgtctgc gccagcgcac
     1021 ctacccattt cgctgccagc ttctccggca gccctgctga cctcgccgct gccacggctg
     1081 ccgcaagttc tgccagcagc aacggcgatg ccatcgtggc cgcggcatgg tacagcggct
     1141 tcttctccgc ttcgatgaaa aatggatcac cacccagcgt cctcacaagc aattccagga
     1201 tcgtgcggat atttttctca gcttctatcg caaacatcac accattcagc gcaaccggcg
     1261 cacgcgtggg aaaactcatc accggagcaa tcgaacccac ttgcgcgccg gcgcgtttcg
     1321 ccgcttccaa taccgtggca ctcagcgctc cgctcgaatg caccacaatc tgcccttcca
     1381 gacttccgcg ttgtgcagca atctgctctg ccacatctcc aatttctgca tcgcgcacac
     1441 agatccagag cacacgcgca tccagcttcg catcttccag gcgcacaaca ggtagcctgg
     1501 accgcgcttc aggcagcgcg cgcaccgcca cttcttttag cgggaccctt gcggacaggc
     1561 agccgtatgc aagcgaagtg ccccaatttc ccaggccaat gatggcgatg tccaactttt
     1621 ttgccatgtc tggatgatac ggtagagaaa aatgcctcac ttaaggaaga acatgccatc
     1681 caagcccgta ctttctgcaa agaaagccga catcgaagtc ctctcatcca ggaccgcatt
     1741 tcaaggcccg ctctttcgcg tggatgtaga caaggtccgc gaacccggta atcgcatttc
     1801 gcgccgtgat gtcatccgcc acaacggctc cgtggtcatt cttgccgtag atgactcgag
     1861 caaaaaagac cccctcatcg tcatggaacg ccagtaccgc cacgcggcag ggcaatatat
     1921 gtgggagatt cccgcgggaa aaatcgaaga gggagaagac cgtctcgccg ccgcaaagcg
     1981 cgaactgcgc gaagagaccg gttacatcgc aaaaaaatgg acgaaactca cgcgctacat
     2041 ccccagcccc ggatttctgg gtgaatggat gcaggtcttt ctcgctgaag gtctgaccgc
     2101 aggagataca tcgccggatg aggacgaatg gctggaagtc gcgctggccc cgctttcaga
     2161 aattctgagt aaaattgaag caggaaaaat tctcgacggc aaaacactca tcgccgtgct
     2221 gttgttcaca catctgcgcg cgaagcgtaa acgctaaatc gcgccattgt atgatctggc
     2281 cgcaaaggct caaccttgca tccaacaatt gattttcgct gaaaaaaaag gaaaaccaaa
     2341 gcgcacgaac atgcgtcttt actttcaagc aggttgtttc ccccaaccgc actctgctta
     2401 agaaggaagg acaaaggctc gtgtctacat acaaaggaaa agtgaagtgg ttcaacaacg
     2461 cgaaaggcta tggatttatc ggacgcgacg atggaccgga tgtatttgtt cactacagcg
     2521 caatccagtt ggacggatac aagagcctga aagaaggcga tgaagtagaa tttgacatcg
     2581 tgcaaggcca gaaaggcccg caggcagacc aggtaattcg cacccgcgaa gtcgcctgaa
     2641 ccaaccgcat tcattcgctc atcttttatt tgcttcgcct gatgcactgc tcgaaagtca
     2701 acaggcgaag tacgcccagg cccagccttt tggctgggtt tcctatttgc gataatctct
     2761 ctcgatggac aatcgctcaa tcgcgcagct tctctatgag accgcagacc tgctggaaat
     2821 cagcgccggc gatccttttc gtattcgttc ctatcgccgt gccgccgaag ctgtggagtc
     2881 gagcaccatc cagctcagcc agatcgctca cgaccccaaa aagcttcagg agattccggg
     2941 catcggcaag ggcatggcag ccaatatcca ggaaattgag aaaaccggga ccctgcccct
     3001 gcgcgaagag cttcttacaa aataccgccc gggaatgctc gacctcctcc ggctccccgg
     3061 catggggcca aaatccgtcg cgctcttctg ggaggccttg cagatatcct ccattgccga
     3121 acttgaagcc gccatcgccg cgggcaaact cgggtccctg ccccgcatgg gcacaaagca
     3181 ggttgaaaag ctgcgcaaag gcattgaaga ctacaaaaag aacagcggac gttttcttat
     3241 agatgacgcc gaaatcgctg cagaaaagct catcgcctac ctccgcaact ttgatggcat
     3301 ggacgtcatc acccccgcag gatcgctccg ccggggtcgc gagaccgtcg gcgatctcga
     3361 catccttgtg acaggccgcg cctgcgcaga agacaaagta caggccgcgg tggattacac
     3421 cgctgcctat ccacctatcg tggatctcat cgccaaaggt cagaacaaag tcagcttccg
     3481 cctccgcggc ggccttcagg tagatgttcg tctgctcccg gaaaattcct acggcgcggc
     3541 cctgcaatac ttcaccggat caaagatgca caacgtcagc attcgtcagc gtgctctcaa
     3601 gcgcggatac accctcagcg aatattctct ggcccgcgtc gaagacggct cattcgtcgc
     3661 cggagcaacc gaagaagaaa tctacgccgc tcttggtctc gactggattc caccggaact
     3721 gcgcgaaaac aacggtgaaa tcgaagccgc tgaacaacac cgcctcccca ctcttatcga
     3781 gttgaaagac attcgcggtg acgtccacat gcacaccaat gcaacagacg gcaaatgctc
     3841 catccgcgaa atggccgaag ccgccgtgca acgcggatac gagtacatcg ccatcacaga
     3901 ccatacaaaa aatctcgcca tgacctttgg cctcgatgac gaacgcgcgc tcgaacacat
     3961 ccggcgtatc cgtgaagtaa atgacgaaat gtcaggccgc attcgcatct tcgccggcat
     4021 ggaagtggac atcctcgccg atggtcagct cgatctcgcc gatgaaaccc ttgcacaact
     4081 cgacgtcgtc atcgtcagcg tccattctct cttcaatatg acggaagaag agatgaccgc
     4141 acgcatcctt cgcgccatcg aaaaccccta tacgcgcatc ctcggccacg ccaccgggcg
     4201 cctcctcctt cgccgcgaag catataaact caacctgccc caaatactgc gccgcgccgc
     4261 tgaactgggc gtcgccgtcg agcacaacgc ctatcctgat cgcctcgacc tgtgcgaccg
     4321 cgacctgcgc ctcgccaaag agctcggctg cagaatcgtc atcaataccg attcacacca
     4381 caccagccac atggaaaaaa tgcgttatgg aataagccag ctccgccgcg catggctgac
     4441 caaaaatgac gtgttgaaca ctctgccccc cgatgaattc ctcaccgcat tacgggcttc
     4501 tcctgcgccg aaatgaaact tgcccctttc cgcagcactc gctactctca tcttgtgggt
     4561 tttcaggatt tcctcggcaa tgaaaccgcc gtacgccacc tgcgtgaagg tctcgcgcac
     4621 ggccgcttgc cgcatgctct catccttgcc ggaccgcgtg gatcaggcaa atacactttg
     4681 gctctcatgc tggcccaggc cgcaaactgc ctgaatccac agcaaaccgg tggtcttccc
     4741 gacttctgcg gtgagtgccg taactgcacc cgcattgccg aatccatgcc tctggaaact
     4801 cgcgtggaag aggccatcgc cgcgcgcgaa gaactgcgcg aagcagacaa aaaagagacc
     4861 cgcatcctcg ttcagacgca ccctgatgtt ctggtcgtcc cgcccgatcc accgcagctt
     4921 ctcattaaat tgggacaggt acgcaccgtc atccgcgaaa tttatcgcat tccatccgag
     4981 gccaaacgcg ccttctacat cttcaccagc gccgccttta tgaaggaggc tgccaattcc
     5041 ctgctgaaag tcctcgagga gccgcccgct cacgccagca tctttctgct tgcggagaac
     5101 acaggcgagc ttcttcccac catccgctcg cgtgcgggaa ttgtgcgcct cggcgcattg
     5161 cccttcgacc agctcgaaca gttgatcgcc cgccgccgct ctgacttgcg ccccgcccag
     5221 cgttccctcg tcgcccggct ggcgcagggg gccgtcggtg ctgcgctggg ttttaacatc
     5281 gaacaatatc tggcccatcg ccaggacgcg ctcatactgc tccgctatgc cacccgcgag
     5341 tcagaccact ctaccctctt tcgcatgacg gaaacgtacc gggccggggc cgaaggccag
     5401 gaaaaaacgg aaggaatgct gcgcgctctc tccaccctgc tggaagacct gctgctgctc
     5461 caaaacggcc agccgggcct tgtccgtaat actgacatcc agggcgaatt ggcttccatc
     5521 gccggaaccg tctcttttga atggatcgaa agcaccgccc gcgccatcca gcaggtgcgc
     5581 tccggaatgc gccgcaatct cctgcgttcg ctggcattgg atgccttcgc cgccgaagtc
     5641 cacgtgtagg ccacggaatt cccccgtcat ccaaaccctg acgcaaaaaa ccacttcccc
     5701 gcgcaaacac gtctgaggta ccctgtgttc catatttcag gaagcctctg agacggccgc
     5761 agaaaagaag gacctcggcg tcatccgccc cttctcattc cctgcgttca gcctcagaaa
     5821 cgatagggtg ttccaggaga aacaatgcca gcagacttta tctccaacct ttacgtcttc
     5881 atccttgccg ggttcgtcgg ctttgagctt atcaaacgcg tttcgccatt gctgcacacg
     5941 cctttgatgt ccttgaccaa cgcccttgat gccatcgtgg tcgtggccgc catcatcatc
     6001 actggaaggc acggctctcc gctgaccacc actttggggg ccatcgctgt tgcggcctcc
     6061 ttcagcaaca tggtgggcgg gttcttcatc accgaccgca tggtccgcat gttcaaatcg
     6121 ggcgggagca ggaaagcatg aacgcggccg ccattcagca atatcttgac ttcgcctaca
     6181 tcgccgccat cgtgctcttc atgctggcac tcaagtggct cagctcgccc gtaagcgccc
     6241 gccgcggcgt ccttgccgga gaaatcgccg ctggcctcgc cgtcctggcc acgctcaaca
     6301 atcctgaagt cgtcacttac aaatggatca tcatcgccct cattcttggc gctggcatcg
     6361 gcattcctct cggcatggtc aaaatcacag ccgtcccgca gcggacggcc ctgagccacg
     6421 ccttcggcgc gctttcagcc gccatgatcg gcatcgccga atactacgaa ggactcgacc
     6481 atctcacgcg ctttggtatg ggtgagattg ccctggaagt catcatcggc tcactcacct
     6541 tcacgggcag tctcatcgcc gcgggcaaat tgcaggaaat ccttccccag cgccccatcg
     6601 tttataaagg ccagaacatc atcagtctct ccgtcctcgc agcggccctc ggactggcag
     6661 ttgcgctcgt catccacccg caacagacaa cgctctttgt cgccatggtc gtcgtagcgc
     6721 ttgtctttgg catcatgctt gtcacgccta ttggcggcgc agacatgccc accgtcatct
     6781 cactcctgaa ctcttacgcc ggtttctccg cggcgctcct aggttttgta ctcaacagca
     6841 agatgctcat cgtcgccggc gcgctcgacg gctcatcggg ttttattctc tccgtcatca
     6901 tgtgtcgcgc catgaaccgc tcctttacca atgttttgtt cggcgcattc ggcaccgtcc
     6961 agaccacggt caccaaaacc gaagcgcgcc ccatccgctc agccagcgcc gaagaggccg
     7021 cgcagatcct cgacgccgcg cgcaaggtcg tcatcatccc cggatacggc atggccgtcg
     7081 cccaggccca gcacaaactg cgcgaactct acgacgtcct cacgaagcgt ggcgtggaag
     7141 tcagcttcgc catccatccc gtcgccggac gcatgcccgg acacatgaac gtccttctgg
     7201 ctgaggccga tgtcccctac gaccgcctca tcgaaatgga cgaaatcaac cctgaatttc
     7261 cgcgcacgga cgtggccctg gtcatcggag caaacgacgt cgtaaacccg gccgctcgct
     7321 tcacaaaagg cagccccatc tctggaatgc ccattctcaa cgcctacgag gcccgcaccg
     7381 tcatggtcat caagcgcagc atgaatccag gcttcgccgg aattgacaac gacctctact
     7441 atcttgacaa gaccctgatg ctcttcggcg acgcgaaagc cttcgtcggc agcctgatta
     7501 aagagctgaa cgccgtcgcc gccggagttt aagcgtcgtc tttgcgccgg cttcgctgac
     7561 tcggtcatcc ttgtaccggg gcgaagccgt cactctttcc gggacgtagc cgaaggccga
     7621 agcacctgct ttgtctgtgt gcatgcgcgt acaaattctg tcatcatcag acagagcaaa
     7681 catgggcctt ttcagcaacg ctcccggcct ctccacaacg ccagcgaccg cccctctggc
     7741 tgaacgcatg cgtccgcgca cgctcgacga atacgttggg caggagcacc tcctcggacc
     7801 gggcaagccg ctgcgtgtcc agattgagcg cgacgatgcc tcctccatga tcttctgggg
     7861 gcctcccggt gtcggtaaga ccacgctcgc caaaatcatt gctgaaacca cacgtgccag
     7921 cttcatcgag tattccgccg tcctctccgg cattaaagaa atcaagcagg tcatggccga
     7981 cgccgaaaag gccgccacct atggctcgcg caccattctt tttgtggatg aaatccaccg
     8041 cttcaacaaa gcccagcagg acgcctttct cccttatgtg gagcgtggct ccatccgtct
     8101 catcggagca accacagaaa atccgtcctt tgagatcatc tccgctctgc tctcccgctg
     8161 ccgcgtttac accctgcgcg ccctgactga ccggcaaata ctcacgctca tcgaacgcgc
     8221 cctcagcgat aaagaacgtg gcctcggcaa tctgaatgtg actgctgatc acgaagcact
     8281 ggaacttctc gccaactacg ccaatggaga cgcccgccgc gccctcaatg ctcttgaagt
     8341 ctccgccgga ctcgcgcagg tctctggtgg ccgcatctcg accgtaacag caacagaagc
     8401 catccagcag cgcgtgctcc tttatgacaa gcagggggaa gagcactaca acctcatctc
     8461 cgctctgcat aaatccgtcc gcaacagtga tgctgacgct gcgctgtact ggctgggccg
     8521 catgctcgaa tccggtgaag acccaatgta tgtcgcacgc cgcctcgtcc gcatggctgt
     8581 cgaggacatc ggcctcgccg cacccgaggc gctgaatctt tgtctttcgg cccgcgatgc
     8641 gatggatttc ctcggctctc cggaaggcga gcttgccctc gcccaggccg ccgtttacct
     8701 tgccctcgcg cccaaatcga atgccctcta tactgcctac ggccaggtcc tctcagacat
     8761 tgaacatacc cgcgcagaac ctgttcccct gcacctgcgc aacgctccta caggattcat
     8821 gaagtccctc ggctacagca aaggttacaa atatgcgcac gatgaagcag gtcgcgtcgc
     8881 cgatatggaa tgccttccgg aaagcctgcg cggacgcaca tactaccatc ccacaaacga
     8941 aggccgcgaa aaactcctcg cccagcgcat ggaagaggtc cgcaaaatca aagaagcaaa
     9001 aaaatcagaa aaagagaagt agaccaacac aaacatcgca acccggcgtg gtctctcaga
     9061 ggctctgcac cg
//
LOCUS       DASRBV010000362         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_133232, whole genome shotgun sequence.
ACCESSION   DASRBV010000362 DASRBV010000000
VERSION     DASRBV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133232"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFA02_13390"
     CDS             complement(<1..883)
                     /locus_tag="VFA02_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exostosin family protein"
                     /protein_id="HZU10891.1"
                     /translation="MARVYLLSVYEDQAYDPNTHYALESLKHSAAIDRFRIHTLVSDA
                     AEADIILFVEMGTTGMFAEVVRAHPLYRRYPQKCFLYDWSDHIFPVIPGIYSSLKKEH
                     FSPEHTRSGFYLLPENPYIQNSPVSGKEKYLAAFVGSLKNHPVRQAFLKFGRDDIYLL
                     DSSKESYRILYHGTPEEKDLFWQRYAASIRDALFSLCPRGLGPGTIRLYESMKMGRAC
                     VILSDEWVPNDGVDWDSFAVRLPESEVHRTPEILDSLRDRAAEMGRRARQEWEDWFSE
                     PVRFHRMVELCLSMKNAR"
     gene            complement(950..1876)
                     /gene="mdh"
                     /locus_tag="VFA02_13395"
     CDS             complement(950..1876)
                     /gene="mdh"
                     /locus_tag="VFA02_13395"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZU10892.1"
                     /translation="MRKKVTIVGAGNVGATAAHWIASKELADVVLIDVVEGIPQGKGL
                     DLLEAMPIEKRDCHITGTNDYADTANSDIVVITAGIPRKPGMSRDDLLQTNYKIMSDV
                     VGKVTAVSPNCILIVVSNPLDAMAQAAFKQSGFNRERVIGMAGVLDSARFRTFIAEEL
                     KVSVENVTAFVLGGHGDTMVPLPRYSTVAGIPITELIEKTKLESLVQRTRDGGAEIVK
                     YLKTGSAYYAPSAATVEMVEAILKDKKKILPCAAYLQGEYGIHGLYVGVPCKLGARGL
                     EQIIEIKLTDDEAAALQKSADAVKELCAVIGV"
     gene            complement(1977..3377)
                     /locus_tag="VFA02_13400"
     CDS             complement(1977..3377)
                     /locus_tag="VFA02_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZU10893.1"
                     /translation="MQTTYNGKPLPQDGKPIEYVNGKYQVPDHPIIPFIEGDGTGRDI
                     WRASQRVFDAAVEKAYGGKRSVKWFEIYAGEKAFREFNTWLPDDTVNAARDLRVSIKG
                     PLTTPVGGGIRSLNVALRQILDLYACVRPVKYYQGVPSPVKHPEKLNVVIFRENTEDV
                     YAGIEFKEGSADAKKLIDFLNNEMLKGGKKKVREDSGVGIKPISITGTKRLVRFAIRY
                     AIENQRKSVTLVHKGNIQKFTEGAFREWGYEVAVQEFRDAVVTERESWILGNVEANPN
                     LTVEQNAALIEPGLEFGSEEFRKNLYAEVKGVLDSIGKSHGNGQWKKKVLINDRIADS
                     IFQQVIIRPDEYSVLACPNLNGDYISDACAAQVGGLGIAPGANIGDGYAVFEATHGTA
                     PKYADKDVINPGSVMLSGVMLFEFLGWNEAAKLIESSMEKTIQQKYVTYDFERQMQGA
                     TKVKTSEFATRMIENM"
BASE COUNT          770 a         1038 c          838 g          813 t
ORIGIN      
        1 gccgcgcgtt cttcatgctg agacacagct cgaccattct gtgaaaacgc actggttcgg
       61 aaaaccagtc ttcccactcc tgcctggcgc gccggcccat ttccgccgcg cgatcacgaa
      121 gtgaatccag tatttcaggt gtcctgtgta cttcggattc aggcaggcga acggcgaagg
      181 agtcccaatc caccccatcg tttggcaccc attcgtcaga caaaatgaca caggcccgtc
      241 ccattttcat ggactcataa agccggatgg tccccggtcc cagcccgcgc ggacaaaggc
      301 tgaacaatgc atctctgatg gacgcggcat aacgctgcca gaaaaggtct ttttcttccg
      361 gcgtgccatg gtacaggatc ctgtaacttt ccttggagga atccagaaga tagatatcgt
      421 ctcgcccgaa tttcagaaat gcttgccgta ccggatggtt tttaagggac ccgacgaagg
      481 ctgcaagata cttttccttt ccgctgaccg ggctgttctg aatgtaagga ttttccggca
      541 aaagatagaa tccggagcgc gtatgctccg gagaaaaatg ctccttcttc agagaggaat
      601 aaattccagg gatgaccgga aaaatgtggt cactccagtc ataaagaaag cacttttgcg
      661 gataacgccg gtacaaagga tgggcgcgca ccacctcggc aaacatcccc gtggtgccca
      721 tttccacaaa aagaataatg tcggcctcag ccgcatcgct tacaagagtg tggatgcgaa
      781 atctgtcaat cgctgcgctg tgcttcaatg actccagcgc ataatgcgta tttgggtcat
      841 atgcctggtc ttcataaaca gacagcaggt ataccctggc cattcgtttt tatctccaca
      901 aaggcgcgga ccatcccacg cctttgtctg aaagatattt ggtggctgct tatacgccaa
      961 tgacagcgca aagctctttc acagcgtctg cgctcttctg cagcgcggca gcttcgtcat
     1021 ccgtcagctt gatttcgatg atctgctcca gaccgcgcgc tccgagcttg cacgggaccc
     1081 cgacgtaaag cccgtgtatg ccatactcgc cctgcagata ggccgcgcac ggaaggatct
     1141 tcttcttgtc cttcagaatg gcctcgacca tttcgactgt ggcagccgac ggcgcataat
     1201 aggcgctccc agtcttcaga tacttcacaa tttcagcgcc accgtcacgg gtgcgttgga
     1261 caagagattc aagttttgtt ttttcaatca gttccgtgat aggaattcca gcaacggttg
     1321 aatatctggg cagcgggacc atcgtgtctc catgtccacc gaggacgaaa gccgtcacgt
     1381 tctcgaccga gaccttcaac tcttcggcaa taaaggtgcg gaagcgggcc gaatcaagca
     1441 ccccggccat gccaatcacg cgctcacgat tgaaccccga ttgcttgaag gccgcctgcg
     1501 ccatggcatc cagcggatta gagacgacaa tcaggataca gttgggcgaa actgccgtga
     1561 ccttcccgac cacatcggac attattttgt aattggtctg gagcagatca tcacggctca
     1621 tgcccggctt gcgcggaatg cctgccgtaa tcacaacaat gtcagagttg gccgtgtcgg
     1681 cataatcatt cgtgccggta atatggcagt cgcgcttctc aataggcatc gcttcgagca
     1741 ggtccaggcc cttgccctgc ggaatgcctt ccactacgtc aatcaaaaca acatcggcca
     1801 gctctttgga ggcaatccag tgcgcagccg tcgcgccaac gtttccggcg cctacaatcg
     1861 ttactttttt acgcattcct tctccttata aaaatcttgt catccaggac aaccgcggtc
     1921 cccgggaaca cgcggttttc gccttccggg ccccggcgaa ggacctgctc attgccctac
     1981 atattctcaa tcattcgtgt ggcaaattcg ctcgtcttca ccttcgtcgc cccctgcatc
     2041 tggcgctcaa agtcgtaggt cacatatttc tgctggatgg ttttctccat tgaagattca
     2101 atgagctttg ccgcttcatt ccagcccaga aactcaaaga gcatcacgcc ggaaagcatc
     2161 accgaccccg gattgatgac gtccttgtcc gcatatttcg gagcagttcc gtgtgtggcc
     2221 tcaaaaacgg catacccatc gccaatgttc gcacctggag cgatgcccag tcccccaacc
     2281 tgcgccgcgc aggcgtctga aatgtagtcg ccattgaggt tcgggcatgc cagtacgctg
     2341 tattcatccg gacgaataat cacctgctga aaaatcgaat cggcgatacg gtcattgatc
     2401 agcaccttct tcttccactg accgttgcca tgcgatttac cgatagaatc cagcacgccc
     2461 ttcacttccg cgtagaggtt cttgcggaac tcttccgagc caaactccaa ccctggctcg
     2521 atcagcgcgg cattttgttc gaccgtgaga ttcggattcg cttctacatt gccgagtatc
     2581 cagctctcgc gctcggtcac cacggcatcg cgaaactcct gcaccgcgac ttcataaccc
     2641 cattcgcgga atgccccttc ggtaaacttc tgaatgttgc ctttatgcac cagcgtgacc
     2701 gatttgcgct ggttctcaat cgcatatcgg atggcgaagc gcaccagccg cttggtcccg
     2761 gtaatcgaga tgggcttaat gccaaccccg gaatcttccc tgaccttctt cttaccgcct
     2821 ttcagcatct cgttgttcag aaagtcaatc agctttttag cgtccgcgct tccctcttta
     2881 aactcaatgc ccgcatagac gtcctccgtg ttctcgcgga agatcaccac attcagcttt
     2941 tccggatgct tcaccgggct gggcacaccc tggtaatact tcaccggacg cacgcacgca
     3001 tacaaatcca gaatctgccg cagggctaca ttcagcgagc ggataccgcc gcccaccggc
     3061 gtcgtcaaag ggcctttaat ggaaacgcgc aggtcgcgcg cggcgttcac cgtatcgtcc
     3121 ggcagccagg tattgaactc gcggaatgcc ttctcgcccg cgtaaatctc aaaccacttc
     3181 acggagcgct tgccgccata ggccttctcg acggccgcat caaaaacgcg ctgcgaggcg
     3241 cgccagatgt cgcggcccgt gccgtcgcct tcaataaatg ggatgatggg atggtccggg
     3301 acctgatact ttccgtttac atactcaatc ggttttccgt cctgaggcag gggcttgccg
     3361 ttatacgtcg tctgcatctt tctggctgtc tccaagcaag attaaggaaa tgagaaactg
     3421 caaaggatga tccacttctg aggctaacat ccgcgaaat
//
LOCUS       DASRBV010000363        10272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_133364, whole genome shotgun sequence.
ACCESSION   DASRBV010000363 DASRBV010000000
VERSION     DASRBV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133364"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VFA02_13405"
     CDS             <1..1311
                     /locus_tag="VFA02_13405"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU10894.1"
                     /translation="LIQNLRAQEASIKAELDQDKVRYGPNYPRIAELQAELNSVERAV
                     RDEVHRIGERARTDYEIAKQAESSARRAFEQQKNLADGLNSKAVAFALAKQEADGSRQ
                     VYENLLAKLKEAGVLEGLRSTNITVVNPARVPSPARPKSPNVPLCYAAAMAAGLFFGT
                     AAAFVRHGADNKVRSVEDAEKMIGAPLLGLVPMIESKPASKKLRSKQTATISYTGSPF
                     AEALRTLRTSLMLSRSSAPPQVLLVTSPGAGEGKSTISLELATVLAQQGAHVLLAEAD
                     LRRPVLSERTGVYHETALSAALSNEAYCPVPQKMEALPNLYVLCSGDTPPFPAELLGS
                     RRMQNLMAQWRCEYDFIVLDGPPVLPVTDAIVLAQQCDAALLVSRHGHTERKALKRSY
                     DVLLRHTPGGVVVGAVLNAVPDGSSDFYDYYGYRSNSYGAKKLA"
     gene            1289..2191
                     /locus_tag="VFA02_13410"
     CDS             1289..2191
                     /locus_tag="VFA02_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU10895.1"
                     /translation="MAQRSWRSSVWFLLLFSATVALRGQAANSNESLLISHGDMLHIT
                     VFREPDLEQHVRVKDSGEIDLDLIGPVRVAGFAPAEAAQKIAQAYVQGRYLNHPQVSV
                     LVEEYATQQVSVLGQVTKPGAVQLMAPRSLLDVLSLAGGLTDIADRHITIQRGDKSES
                     MTVFLPNNAQADLQSTVTVYPGDTVIVPKAGIVYVLGDVARPGGYIMQDDAKLTVLQA
                     IAMAAGANRSAKEDATRLLRKVNGKYQERTVLLHEIERGKLPDMELEADDVLYVPFSM
                     AKNVMLGASGIVSSTSSAAIYATR"
     gene            2178..3497
                     /locus_tag="VFA02_13415"
     CDS             2178..3497
                     /locus_tag="VFA02_13415"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZU10896.1"
                     /translation="MQLDEVRFGPAELLCVIILAFFAMQGAIPGIAPNQAAEMTNTAA
                     TPLMKVAGIGSQIIVNGLIAVLVVHQWRKLYRLRFALQWSAALALLAMASALWSQAPA
                     TTARRAVPFALATLFGLYFAGRFSLRRQLSILCFTMTIAALASAVLALAFPSIGLEAS
                     SGHFGNWQGVFTQKNACGRAMVFAIAAVLSYGKWNAVRLACFVLFAFVLAMSGSRGAW
                     VIAATILVLYVLLLVLERFTSASRLVVLCVAVVLMAAAAMMAWAYFPVFALFLGRDAT
                     LTGRTAIWQQVWAAILKHPWLGYGFSAFWQGMKGESYNVILALRFVLFHAHNGFLEVW
                     LELGAVGLLLFTLGHLRAWRRLWPLLLKRHMPECAWMFFVLALIFLYDLDENTLLTFN
                     GAFWVLYVAAVADIEILAAEQDIAHALVAAQKRASSPSLSAGAIAAR"
     gene            3494..4621
                     /locus_tag="VFA02_13420"
     CDS             3494..4621
                     /locus_tag="VFA02_13420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU10897.1"
                     /translation="MTATVQAPRAVAVRFYHPELDALRFFAFLAVYICHAIPAASARV
                     ALIADIKDAGNFGVCLFFLLSAYLITELLRRELEEFGTIAIRAFYLRRILRIWPLYFG
                     VIVSYAMGGIFFHALRMETGRMIAYFLLAGNWYIALHPWIQTPLRALWSISVEEQFYL
                     VWPLLGKFGGMRAFMLAAIVLTPFSLMTVFIVAGAPYAWNTVWLNSFAQFLFFACGAL
                     LALALKGQLPGLRTWVRAALAIMGLLAWWVSASAIQIKRPGAHVAPAQFCIGYVLVAC
                     GCICLFLSVLGIEEHFVPPWMAYLGKISFGLYVFHETAFLLVDEVQKHALRRDAAWLL
                     VLNKIVALGATVFLAWVSYRFYESPFLRLKQHYARVPSRSV"
     gene            4622..5821
                     /locus_tag="VFA02_13425"
     CDS             4622..5821
                     /locus_tag="VFA02_13425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU10898.1"
                     /translation="MRRVIIYRNELLPASETFVPAQANALQRYAPVFAGIRRVKSGLS
                     ITACPVITVSPSEHVWKKLLRRIFLRTGRARAFQNAIVSYRPDLIHAHFATDACAVLP
                     LAQQFRLPLVVTLHGYDVCCDDQAFRHWPTTRAYLRRRKALWQYASLFLCVSEHVRRQ
                     AILRGFPQEKLSVHHIGVSTRCAPVGAGQRGPATVLFVGRLVEKKGCNYLVRAMSGIV
                     REVSDAKLIIIGDGPLRNALEHEALPLGERVVFLGQQSHDQVMQWMRSARVLAAPSVC
                     ASDGDQEGLPTVLCEAQALGLPVVTFATNAVTEALPGELRNAMPYERDEAGLARAVVR
                     LIQDGAQWKHASALGRQYMEEQFDIERQTRTLEEIYDKVIADPVDKVRGSGTGRASDM
                     PEVLPVP"
     gene            complement(5828..8335)
                     /locus_tag="VFA02_13430"
     CDS             complement(5828..8335)
                     /locus_tag="VFA02_13430"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZU10899.1"
                     /translation="MSATKLEPAHSELQQATQNREQVFDAFRRWGYLQAQLDPLGQYL
                     TPEPVPDLDYEGEYAEEARSYYCGTIATEFMHIPYRERRQWIAERMESEPPAADQQRI
                     LDLLIKADIFEQVIQSRYLGTKRFSLEGVTALIPFLDELLNRAAELGAEKAVMGMSHR
                     GRLSVMVNTFGKSPADIFSKFEDVDPRSILGGGDVKYHVGATGTYETRDGKTLALHLV
                     SNPSHLEAVDPVVMGRARAKQARYGAEGIDRVLPLAIHGDAAFAGQGIWAETLNLGNV
                     EGFSVGGSIHIIVNNLLGFTADPEESNSSRFSSDIAKRLPIPIFHVNAEDPDAVVRLA
                     ALAAEYRYTFKSDVVIDLIGYRRHGHSEVDDPTITQPLRYAKIKEHPPLYEIYAKKLG
                     ADISGRVRELQTEFAEAQRTATKMKKMPTLAVLPDYWSQFKGGKYKPEYEVETGLTKD
                     EIRAIAEGISSAPESFHVHPKIQKLLEQRRKMGEGKLPFDYGMAEAVAIGSLLKSGVP
                     VRLSGQDSQRGTFNQRHSVLVDIENEQVFVPLNHLTENQAKFEVYNSVLSEAAVLGYE
                     YGYARDFPEALVMWEAQFGDFANGAQIIIDQFISSGEDKWGLLAGVVVLLPHGYEGQG
                     PEHSSARIERYLQLAAHDNMQICQPSTAAQYFHLLRRQALRKWRKPLIVFTPKSMLRH
                     PDAVSPIEELERPHFQNVLPETKIMNAQRLLVCTGKIGHELRVEREKRKDTTTGIIFV
                     EQLYPWPEAELESAIKAHPEAHEVIWVQEEPENMGALSFVMPRLKRLVRDRQVLSIKR
                     SAAASPATGSAKAHEMEQKTLIGLALGPGLREKAAQR"
     gene            8531..9775
                     /locus_tag="VFA02_13435"
     CDS             8531..9775
                     /locus_tag="VFA02_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10900.1"
                     /translation="MSSSLTASAEKVPETPSSGGLLRVLAVISFCHLLNDMVQALLPS
                     IYPILKSSFHLNFGQVGFLTLTYQITASLLQPLAGHYADRKPRPFSLAFGMGSTLCGL
                     VLLAVAPTFGWLLLAASLVGVGSAIFHPESSRVARMASGGRHGFAQSFFQVGGNTGSA
                     IGPLLAAFIVLPHGQKGIAWFAIPAVLGIFVLTRVGVWSKHHQALAAFRKSAREMATR
                     QTLSGKRVIGALTVLVALIFSKYFYLASLTSYYTFYLISHFHVSVRTSQVFLFIFLAA
                     VAAGTMIGGPVGDRYGRKYVIWVSILGVLPFTLLLPHANLLWTGILSVIIGFVISSAF
                     SAILVYAQELVPGRIGMISGVFFGFAFGMGGIGAAVLGRLADATSIVFVYKLCAYLPA
                     IGLLTGLLPDLEPGHKGVFRKA"
     gene            complement(9870..>10272)
                     /locus_tag="VFA02_13440"
     CDS             complement(9870..>10272)
                     /locus_tag="VFA02_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU10901.1"
                     /translation="ARSSDEKQERRWHLFASLTGAAAGFGLSVVLQSPWVAMSALFLA
                     AAGVWGCFGPFWALPTAMLRGTAAAGGIAIINSVGALGGFCGPYLMGRVSDATHSFHA
                     ALAASTGLLLIAALVAIRLRIQVPKKINYTS"
BASE COUNT         2031 a         2726 c         3003 g         2512 t
ORIGIN      
        1 ctgattcaaa accttcgcgc acaggaagca agcatcaaag cagagcttga ccaggacaag
       61 gtgcgctatg gaccgaacta tccgcgaatt gcagaattac aggccgagct gaacagtgtg
      121 gaaagggctg tacgcgacga agtgcatcgc attggcgagc gggcgcgtac agactacgaa
      181 attgcaaagc aggctgagtc ttctgcccgc cgggctttcg agcagcaaaa gaatctggcc
      241 gatggactga acagcaaggc cgtagcattt gctctggcga agcaggaagc agacggaagc
      301 cgccaggtct atgaaaatct tctggcaaag ctgaaagaag cgggtgtgct tgaagggttg
      361 cgctcgacga acattacggt agtgaatccg gcgcgtgtgc cttctccggc gcgtcccaaa
      421 agccccaacg tgcctctttg ctatgcagcg gccatggcag caggattgtt ttttggcact
      481 gctgctgcgt ttgtgcggca tggtgcagac aacaaagtgc gatccgttga agatgcagaa
      541 aagatgatcg gagcgccact gctggggctc gtccctatga tcgaaagcaa accggcaagc
      601 aagaagcttc gttcaaaaca gacggccacc atcagctaca caggttctcc gtttgccgag
      661 gcgctgcgga cgctgcgcac atccctgatg ctttcaagaa gcagcgcgcc gccacaggtc
      721 ctcctggtta ccagcccggg ggctggcgaa gggaaaagca caatttcact ggagctggct
      781 acggtgctgg cgcagcaagg cgcgcatgtc ctgctggcag aagcagacct gcggcgccca
      841 gtcttatcgg aacgtaccgg cgtataccat gaaacggcgc tgagcgcggc gttgtcgaac
      901 gaagcgtatt gtccagtgcc gcaaaagatg gaggcgctac cgaacctgta tgtcttgtgc
      961 agcggtgata cgccgccatt ccctgctgag ttgctcggat cgcgaagaat gcaaaatctt
     1021 atggcgcagt ggagatgcga atacgacttt attgttctgg atgggccgcc cgtgctgccc
     1081 gtgacggacg ctatcgtgct ggcgcagcaa tgcgatgctg cgttattggt ctcgcgccat
     1141 gggcacactg aaagaaaggc gctgaagcgc agctatgatg tgctcctgcg tcatactcca
     1201 ggcggtgttg tggtcggcgc agtgctgaat gccgtgcctg atggctcctc agatttttac
     1261 gattactacg gctaccggag taacagctat ggcgcaaaga agctggcgta gctctgtatg
     1321 gttcttgctg ctcttcagcg cgaccgttgc tttgcgcggg caggcagcaa acagcaatga
     1381 aagtcttctg atttcgcacg gagacatgct gcatatcaca gtctttcgtg agcctgacct
     1441 ggagcagcat gttcgcgtaa aggactccgg tgagattgat cttgatctga ttggaccagt
     1501 ccgcgtggct ggatttgctc cggcagaggc agcgcaaaag atcgcgcagg catacgtgca
     1561 gggcagatac ctgaatcatc cccaggtctc agtactggta gaagagtacg cgacgcagca
     1621 ggtttcggtg ctggggcagg tgaccaaacc aggcgctgtg cagttgatgg cgccgcgaag
     1681 tcttctggac gtgctctcgc tcgccggagg attgactgac attgcagacc gtcacatcac
     1741 tatccagcgc ggagacaaga gcgaaagcat gacggtattt ttgcccaaca atgcacaggc
     1801 cgacctgcaa agcacggtga cggtgtatcc gggagacacg gtcattgtgc ctaaggcagg
     1861 catcgtttat gtgcttggtg acgttgccag gcccggtgga tacatcatgc aggacgacgc
     1921 aaagctcacg gtgctccagg cgattgcgat ggctgcgggc gcgaaccgct cggcaaaaga
     1981 agacgcaacg cgcctgcttc gcaaagtcaa tggaaagtat caggagcgga cggtccttct
     2041 ccacgagatt gagcgcggca aactgcccga catggaatta gaagcggacg acgtccttta
     2101 tgttcccttc agcatggcga aaaacgtgat gctgggggct tcgggaattg tgtcctccac
     2161 cagttcggcg gcaatttatg caactcgatg aagtgaggtt cggcccagcc gaactcctgt
     2221 gcgtcatcat tctcgctttt tttgccatgc agggcgcgat acctggaatt gcgccgaacc
     2281 aggcggccga gatgacaaat acagcggcaa ctccactgat gaaggttgcc gggatcggct
     2341 cgcagatcat cgtgaacgga ttgattgctg tactggttgt tcatcaatgg cgcaaacttt
     2401 accgtctgcg gttcgccctg caatggagcg cagcattggc attgcttgcg atggcatcag
     2461 ctttgtggtc acaggccccc gcaacgactg cccggcgggc tgttccgttt gcgctggcaa
     2521 ccctttttgg cctgtacttt gcgggccggt tctctcttcg caggcaactc tcgatccttt
     2581 gcttcacgat gaccattgca gctttggcga gcgcggtgct ggcgctggca tttccttcca
     2641 ttggactgga ggcgtcctca gggcacttcg ggaattggca gggagtattc acgcagaaaa
     2701 atgcgtgtgg ccgcgccatg gtttttgcca tcgctgctgt tctttcctat ggcaagtgga
     2761 atgccgttcg ccttgcatgc tttgtgctgt ttgcgttcgt gctggcaatg agcggctcgc
     2821 gaggggcctg ggtcatcgcg gccacaatcc ttgtgttgta tgtgcttttg ttggtactcg
     2881 aaagattcac ctctgcaagc cgtctggttg ttctgtgtgt cgccgtcgtc ctgatggctg
     2941 ctgccgcaat gatggcgtgg gcatacttcc ctgtgttcgc cctctttctg ggcagggatg
     3001 ccacgcttac tggacgcacc gccatctggc agcaggtgtg ggcggccatc ctcaaacatc
     3061 cctggcttgg ttacgggttt tcagctttct ggcagggaat gaagggagaa tcctacaacg
     3121 tcatactagc cctgcgtttc gtgctctttc acgcgcacaa tggatttctt gaggtatggc
     3181 ttgagcttgg ggccgtcgga ttgttgttgt ttacgctggg ccatctgcgt gcctggcgaa
     3241 ggctctggcc gctgctcttg aaacggcaca tgccggaatg cgcatggatg ttcttcgttc
     3301 ttgcgctgat atttctctat gacctggatg aaaacacgct gctgaccttc aatggggcct
     3361 tctgggttct gtatgttgct gcggttgctg atattgagat actcgctgca gagcaggaca
     3421 tcgcgcatgc gctggtggca gcgcagaaac gggcatcatc gccatcactt tctgccggag
     3481 cgattgctgc aagatgacgg ccactgttca ggcgccgcgc gcggtcgctg tccgcttcta
     3541 tcatcctgag ctggacgctc tgcggttctt cgcgtttctt gctgtatata tctgccatgc
     3601 aattccggcg gcttcggccc gggtggctct gattgcggac atcaaagatg cgggaaattt
     3661 cggggtctgc ctgttttttc tgctgagcgc gtatctgatt acagagctgc tgcgccggga
     3721 gttggaagaa tttggcacga ttgccatccg cgcgttctat ctccggcgca tccttcgcat
     3781 ctggccgctc tactttgggg tcattgtctc ctatgcgatg ggcggcatct tttttcacgc
     3841 tctgcgcatg gagacgggac gaatgattgc gtactttctg ctggcaggga actggtacat
     3901 tgcgctgcat ccctggatac agacgccatt gcgggcgttg tggagcatct cggttgaaga
     3961 gcagttctat ctggtctggc cattgctggg gaagtttggc ggaatgcgtg cttttatgct
     4021 ggctgcgatt gtgctcactc cgttttctct gatgactgtg ttcatcgttg caggtgcccc
     4081 ttatgcctgg aacactgtat ggctcaacag ctttgcgcag ttcctctttt ttgcatgcgg
     4141 agcgttgctg gcgctggcgc ttaaagggca acttccagga ctaagaacgt gggtgcgggc
     4201 ggcgctggca ataatgggtt tgctggcctg gtgggtgtcc gcttctgcaa ttcagatcaa
     4261 aagacccggt gcacatgtgg cgccggcgca attttgcatt ggatatgtgc tggttgcatg
     4321 cggctgcatc tgcctgtttc tttctgtcct gggcatcgag gagcattttg tcccgccatg
     4381 gatggcctat ttgggaaaga tttcctttgg tctgtacgta tttcatgaga cggcctttct
     4441 gcttgtagat gaagtgcaga agcatgccct gcgcagagat gctgcatggc tgcttgttct
     4501 gaacaaaatc gtggcattgg gcgcgaccgt cttcctggca tgggtatcct atcgctttta
     4561 tgaatcgcct tttctgcgtc tgaagcagca ttatgctcgc gtgccatcaa ggagcgtgta
     4621 ggtgcgcagg gtcattatct atcgcaatga gcttctgcct gcttcggaaa cctttgtgcc
     4681 ggcacaggcg aatgccttgc agcgatacgc cccggtattc gcgggcatca ggcgcgtgaa
     4741 gtcaggactg agcataacgg cctgcccggt aatcacggtc tcaccgtctg agcatgtctg
     4801 gaaaaagctt ctccgcagga tttttctgcg caccggccgc gcgcgcgctt ttcagaatgc
     4861 aattgtgtcg tacagaccgg acctcatcca tgcccatttt gcaacagatg cctgcgctgt
     4921 tttgccgctt gcgcagcaat tccggctgcc tctggttgta acgctgcatg ggtatgacgt
     4981 ctgctgcgat gatcaggcat ttcgccactg gcccacgaca cgggcctatc tgcgcagaag
     5041 gaaagcattg tggcaatacg ccagcctgtt tctttgcgtt tcagagcatg tgcgcagaca
     5101 ggccattctg cgcgggtttc cgcaggaaaa actatcggtg catcacattg gagtttctac
     5161 aagatgtgcg ccagtcggcg caggtcagcg ggggcctgca accgtcctgt ttgttggcag
     5221 gctggtggaa aagaagggct gtaattatct ggtccgcgcc atgtccggaa tcgttcgaga
     5281 agtttcggat gcgaagctca tcatcatagg agacggcccg ctccgaaacg ccctggaaca
     5341 cgaggccctg ccactcggtg aaagggttgt ttttctcggg cagcaatctc acgatcaggt
     5401 aatgcaatgg atgcgcagcg cgcgcgtgct ggctgctccc agtgtttgcg cttctgatgg
     5461 cgaccaggaa ggcctgccga cggttttatg tgaagctcag gcgctggggc ttcctgtagt
     5521 gacatttgca actaatgcag ttacagaagc gcttcccggt gagttgagaa atgcaatgcc
     5581 ttacgagcgc gacgaagctg gccttgcgcg tgcggttgtt cggctcattc aggatggcgc
     5641 gcagtggaag catgcaagcg cgttgggaag acagtacatg gaagagcagt ttgatatcga
     5701 gcggcaaacg cgcactcttg aagaaatata tgacaaggtg attgcggatc cggtagataa
     5761 agtgcgaggg agtggtacag gaagggcctc agacatgcct gaggtccttc ctgtacctta
     5821 ggtccaatta tcgctgggcg gccttttcgc gaaggccggg gccgagcgca agcccgatca
     5881 gcgtcttctg ttccatctcg tgcgctttgg ctgatccggt tgccgggctg gcggctgccg
     5941 agcgcttaat actcaacacc tggcggtcgc gaacgagccg cttgaggcgc ggcatcacaa
     6001 acgacagcgc acccatgttc tccggttctt cctgcaccca gatgacctcg tgcgcttcag
     6061 gatgtgcctt gatggcggac tcaagttcgg cctcgggcca cggatagagc tgttccacaa
     6121 agataatgcc tgtggtcgtg tctttgcgtt tttcgcgttc gacgcgaagc tcgtgtccga
     6181 tttttcccgt acagacaagc aaacgctgcg cgttcataat ctttgtttcc ggcagaacat
     6241 tctggaagtg cggacgctca agttcttcaa tcggggagac cgcatccgga tggcgcaaca
     6301 tgctcttcgg cgtaaagacg atgagcggct tgcgccattt ccgcagcgcc tggcgccgca
     6361 ggaggtggaa gtactgtgct gccgttgacg gctggcagat ctgcatattg tcgtgcgccg
     6421 caagttgcag atagcgttcg atgcgcgcgc tggagtgctc aggcccctgg ccttcatatc
     6481 catggggaag cagaacaacc actcccgcaa gcagtcccca cttgtcttcg cctgaagaga
     6541 tgaactggtc aatgatgatc tgagcaccat tggcaaagtc gccaaactgc gcttcccaca
     6601 tcaccagcgc ctcgggaaag tcccgggcat agccgtattc gtagccaagt accgccgctt
     6661 cagaaagaac agagttgtag acctcgaact tcgcctggtt ctctgtcaga tgattcagcg
     6721 ggacgaatac ctgctcattt tcaatatcaa ccaggacgct gtggcgctga ttgaatgttc
     6781 cgcgctggct gtcctgcccg ctcaggcgga caggcactcc agacttaagc aatgaaccga
     6841 ttgcaactgc ttcggccatt ccgtagtcaa agggcagctt gccctcaccc atcttcctgc
     6901 gctgttcgag cagcttttga attttggggt ggacatgaaa actttcgggc gcgctggaga
     6961 ttccttctgc aatcgcccgg atttcgtcct tcgtcagccc ggtttcgact tcatactccg
     7021 gcttgtactt accgcctttg aactgtgacc aatagtcagg cagcacagcc agtgtcggca
     7081 tctttttcat ctttgtggca gtgcgctgcg cctcggcaaa ttcagtctgg agttcgcgta
     7141 cccgtccgct gatgtctgcg cccagcttct ttgcgtaaat ttcatagagt ggcggatgct
     7201 ctttgatctt cgcgtagcgc agaggttgcg tgatggtcgg gtcgtctact tcgctgtgtc
     7261 catgacggcg atagccaatc agatcaatga caacatcgct cttgaaggtg tagcggtatt
     7321 cggcggccag tgcggccagg cgaacaactg catccgggtc ttccgcattc acgtggaaga
     7381 tggggatcgg caggcgcttt gcaatatcgg aggaaaaacg cgatgaattt gattcctcgg
     7441 gatcggcggt gaatccgagc aggttattca cgatgatgtg aatgcttccg ccaaccgaga
     7501 agccctcgac attgccgagg ttgagcgttt ctgcccagat accctgtccg gcaaaggccg
     7561 cgtctccgtg gatggccaga ggcagcacgc ggtcaattcc ttcagcgcca tagcgggcct
     7621 gcttggcgcg tgcgcggccc atcacgactg ggtctaccgc ttccagatgg ctgggatttg
     7681 agaccagatg caaagcgagt gttttgccat cgcgggtttc ataggtgccg gttgcgccaa
     7741 cgtggtactt cacatcgccg ccgccaagaa tgctgcgcgg gtccacatct tcaaatttag
     7801 agaagatgtc tgccggagat ttgccgaagg tattgaccat gacgctgaga cgtccacgat
     7861 ggctcatgcc catgacggcc ttttcggcgc cgagttcagc agcgcggttc agcagttcgt
     7921 caagaaaggg aataagagcg gtcacgcctt ccagcgaaaa gcgctttgtt cccagatagc
     7981 gcgactgaat cacctgctca aaaatgtcgg ccttgatcag aaggtcgaga atacgctgct
     8041 ggtcagcagc aggtggttct gattccattc gctctgcgat ccattggcgg cgctcgcggt
     8101 aaggaatgtg catgaactca gtagcaatgg tgccgcagta atagctgcgc gcctcttcgg
     8161 catattcgcc ttcgtaatca aggtctggga ccggttcggg agtgaggtat tggccgagag
     8221 ggtcgagttg ggcctggagg tatccccagc ggcggaaagc gtcaaaaact tgttcgcggt
     8281 tttgggttgc ctgttgcagt tcagaatgtg cgggttccag ctttgtagca ctcataaata
     8341 tcgtgtccga aaatcgccgg cgtaaacaat cgaattgtcg ggataaattt gcccggaaaa
     8401 atggccagag atttcatcgt ctcttttatt ttactggttt ggagaaggtt gtgggattcg
     8461 gtgcgcaaaa gctgttctcg caaaccggaa tttgtatcga acttccgtac catagaatct
     8521 aacgaacgtc atgagctcct ctcttacggc cagtgctgag aaggttcctg aaaccccgtc
     8581 ttccggcgga cttctgcgag tattggcggt cattagcttc tgccacttgc tcaatgacat
     8641 ggtgcaggcc ctgctccctt cgatctatcc cattctgaaa agctcatttc atctgaattt
     8701 cggacaggtt gggttcctta cgctgaccta tcagattacg gcctcactcc ttcagccgct
     8761 tgccggacac tatgctgacc gcaagccgcg gccgttttcg cttgccttcg gaatgggttc
     8821 tacactctgc ggactcgtgc ttctggccgt agcgcccacc tttggctggc ttctgctggc
     8881 tgcatcgctg gttggagtag gttctgcaat ctttcatcct gagtcatccc gcgtggcccg
     8941 catggcttct ggcgggcggc atgggttcgc gcagtcgttt tttcaggttg gtggaaacac
     9001 aggatcagcc atcggcccgc tgcttgcagc ctttatcgtc ttgccgcacg gccagaaggg
     9061 aatcgcgtgg ttcgccattc ccgcagttct cggaattttc gtgctgacta gggtcggcgt
     9121 atggtcaaag caccatcagg ccctggctgc ttttagaaag agcgcccgag agatggccac
     9181 acggcagacg ctttcaggaa aacgcgttat cggcgcgctg actgtgcttg ttgcgctgat
     9241 tttttccaag tacttctatc tggcgagcct aacaagctat tacacgtttt atctcatcag
     9301 ccactttcat gtttccgtac gcacgtcaca ggtctttctc tttatctttc tggctgcggt
     9361 cgcggccggc acgatgattg gcggccctgt cggcgacagg tatggaagaa aatatgtcat
     9421 ctgggtttcg attctcggag tacttccgtt cacgcttctg ctgcctcatg caaatctcct
     9481 ctggacaggc attctcagcg tgattatcgg ttttgtgatt tcatcggcct tctcggccat
     9541 tcttgtttac gcgcaggaac tggttccagg acgcatcggc atgatctccg gggtcttttt
     9601 cggcttcgcc ttcggtatgg gcggcattgg agcagccgtg ttgggcagac tggccgacgc
     9661 aacgagcatt gtctttgtct ataaactttg tgcgtatctg cctgcgattg gcctgctgac
     9721 aggacttctt cctgacctgg aaccgggaca taaaggcgtc ttcaggaaag catgaatcct
     9781 gtgtgatgta cagcctctaa agttcatgac gtaaactatg tagacatttg ctaccttcag
     9841 cattgaaaag ttcgttcatg ttgtccatct tagcttgtat agttgatttt cttggggact
     9901 tgtatacgaa ggcggattgc caccagggct gcaatcagaa gcaacccagt ggaggcggcc
     9961 aatgcagcat ggaagctatg cgtagcgtcg ctgacacggc ccattagata tgggccgcag
    10021 aatccgccaa gcgcaccgac ggaattgatg atggcaatgc cgcctgccgc tgcggttccg
    10081 cgcagcatgg cggtgggcag tgcccagaat ggcccgaagc atccccatac gccggccgcg
    10141 gccagaaata aggcggacat ggccacccag ggactttgca gtacaacact cagaccgaat
    10201 ccggctgctg cgcccgtgag tgaagcgaaa agatgccatc ggcgctcctg cttctcatct
    10261 gaagagcgtg ca
//
LOCUS       DASRBV010000364         5833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_434146, whole genome shotgun sequence.
ACCESSION   DASRBV010000364 DASRBV010000000
VERSION     DASRBV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434146"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFA02_13445"
     CDS             complement(<1..1127)
                     /locus_tag="VFA02_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10902.1"
                     /translation="MSLNGDQLYALLPAVYRTRDAAEGGPLQSLFAVIAQQAAVIENN
                     IEQLYDDQFIETCAQWVVPYIGDLIGYNSIYDVAAANIESRSEVANTIGYRRRKGTRI
                     ALEQVAMDVSGREALAVEYFKRLITTESMRHVRPHHVATVNVRHEGTLETIATAFDTQ
                     NRTVDVRRIAPRNRIAADPDPTPLDIALHGPGRFNIPDVGIWLWRWQSWPVTNAPAFS
                     LGNGRFLFSSLGMNIPLFPLPAVRASFSHLTTRKDVPQPITRREFCKTPGAFYQNNSR
                     PGIQLFADGLPVPLEHICCCNLSTWCHVPSGMIGMDPELGRIQFASDLPLPQSLRVNY
                     SYGFPAPLGGGPYDRSASLPLTDPSKLNFFAVAGSAGTPTL"
     gene            complement(1124..3982)
                     /locus_tag="VFA02_13450"
     CDS             complement(1124..3982)
                     /locus_tag="VFA02_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HZU10903.1"
                     /translation="MNGECVIQDQQLCGCCEGMGRETPQVIYNRPALSAIVYRAGTYS
                     SFRASLLASLSDPDYPALALLRTRDSSDFTIALLDAWSVVCDILTFYQERSANEAWLR
                     TAVDQRSVFELARLVGYVPSRGVAASDILAFTLSSAPGSPDNVLIPTGTRVQSIPGPG
                     QTPQVFETSADLTAVIAWNALPAQTARPWQLSPSDTSTWIEGTANNISVGDALLFVSS
                     AAGKPPSKNIGDVHYVTHVFVDPDAKRTKITWDNTFTSELQGALDNVSVYVFRRKAAL
                     YGVQAPAMPLIPNGENSGIDATKITGYVAGGTDWDFTRTFKENSGQINLDASYSGLEA
                     GQWVVLTGLGYTSFFQITAAPSESNPNAFTLTTKTTQLTLDLGEIIYGDKALSLNEVL
                     WEFVKETRDITAYIQSVQLTPTTLPLTGWALDGSYTKQSGMLAPVEGTTLSIVGGQKI
                     ASNQPIGVSGKRVRLQVVTGASAIFTPANAAGALTVSDGQIFLIDSFPPVNDLWSVIT
                     LSGVPGSLLTDTGNVTLLPSDSGDPTVGEAAIVQSTSVQGDTTTLSLVQPLGRIYDRA
                     TVNVNANAVDATHGETVQEILGNGDATNDALEFTLKQAPLTYVSASSGNGSQSTLEVW
                     VNNLQWHEVPNFLSSGPADRVFVTRVNAAGNVVIQFGNGTNGSRTPTGQANIRAVYRK
                     GIGIAGMVQAGQLSQPLDRPQGLKSATNPSPASGAADPASSDDARTSAPLPTLTLNRI
                     VSLEDYQNYALAFAGIAKALATWTWFGNTRGVYLTVAGENGAVLHPDDDIITHLVQAL
                     RSQGNPYVPLKVVSYAPTLFTFTAKIRVDSDYSPPLVLADVWQNVSNAFSFRARQPGQ
                     NVVASEIIELMQQTPGVVAVQLQSLGLSGEPLSSIVPPVLCASSPTVRSLVPIFRFPL
                     RRFSTLTFLHPTTVNQAPQPAQMLLIDPATIGNLGVWS"
     gene            complement(3979..>5833)
                     /locus_tag="VFA02_13455"
     CDS             complement(3979..>5833)
                     /locus_tag="VFA02_13455"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HZU10904.1"
                     /translation="SYQQDAVSTEAYIGTARSRISLRRHARLVDYYLNEGCNARAWVA
                     LSVSANITLPQGTHFYARVPGLPASAKPGSTAAQQLAKTTQPIFASMADATLYPEQNE
                     MQFYTWSDTNCCLPPGATEATLAGNFTSLKKGTILVFEEVAGPQTGNSADADPTHRWA
                     VRLIHDSSVLMDPLTLKSVTRIEWAQEDALPFPLCISSTFTDADGKTVFVPAVSVAHG
                     NIIPVDHGVWLDRWEDLREVPAAPPAPNPATSCSCSGQPGPPAGRPRYYPELQNSPLT
                     FALPFDASAPASAFLAPPATAVAAQPQIQIQSDDGQFWTIPSPPDLLSSNENERVCVP
                     EIEHNGSVFLRFGDGQYGMAPEQGQHFYALYRVGNGSAGNIGRDTLAHVVTPVPGITG
                     VRNPLAAAGGTDPEDMEHIRQYAPFSFQTQLRAVMEDDYGTQAMTVAGVSEARGTLRW
                     TGSWHTAFVSVSPAKAMSPALITAVNDTLNMRRMMGVDIESEGAKIVGLRIEMEICVA
                     ADHFQSDVEEALLHLFITGNQCSGQPGLLNPKNFTFGQTVYASPLISAAQSVEGVVSA
                     TLTVFERMDDPSVDGVAQGQLTMGRLEIARCDNDPNRLDHGIFVLHMDGGK"
BASE COUNT         1278 a         1533 c         1762 g         1260 t
ORIGIN      
        1 tcaagtgtgg gtgtcccggc tgatccggct acggcaaaga aattcagctt tgaagggtct
       61 gtcaacggca gactggcgga gcggtcatac gggccgccgc caagcggcgc tggaaatcca
      121 taggagtagt tcacgcgtag cgattgtggc agtggaagat cactggcgaa ctggatgcgt
      181 ccgagttcgg gatccatgcc aatcatgcct gatggaacat ggcaccaggt gctgagattg
      241 cagcagcaga tgtgctcaag tggaacaggc agaccgtctg caaaaagctg aataccgggc
      301 cggctgttgt tttggtagaa agcaccggga gttttgcaga actcgcgccg tgtaatgggc
      361 tgtggaacat ctttgcgcgt agtgaggtga ctgaatgaag cacgaacagc aggcagcgga
      421 aacagtggaa tgttcatccc cagcgaactg aacaggaagc gtccattgcc gagagaaaag
      481 gctggggcat tcgttaccgg ccagctttgc caccgccaga gccagatgcc cacgtcggga
      541 atattgaagc ggccagggcc atgcagggcg atgtcgagcg gtgtgggatc ggggtcggct
      601 gcaatacgat ttcgcggagc gatgcggcgc acatcaaccg tgcggttctg cgtgtcgaat
      661 gcggtagcga tcgtttcaag cgtgccttcg tgtcgcacgt tgaccgtcgc aacatgatgc
      721 ggacgcacat ggcgcatgct ttcagtggtg atgagccgct tgaaatattc aacagccagg
      781 gcctcacggc ccgagacgtc catcgctacc tgctcgagcg cgatgcgtgt tcctttacgg
      841 cggcggtaac cgatcgtatt ggcgacttca gagcggcttt caatgtttgc ggctgcgaca
      901 tcgtaaatgg agttgtagcc gataagatcg ccaatatagg ggaccaccca ctgcgcgcat
      961 gtttcgatga actggtcatc gtaaagctgt tcgatgttgt tttcgatcac cgcagcctgt
     1021 tgtgcgatga cagcaaaaag cgattggagc gggcctcctt ctgctgcgtc gcgagtgcgg
     1081 tagaccgcag gcagtaaggc atacagttga tctccgttga ggctcatgac cacactccga
     1141 gattgccgat ggttgcagga tcaatcagaa gcatctgcgc aggctgcggc gcctgattga
     1201 ccgtggttgg atgcagaaag gtcaaagttg aaaaccgccg gagaggaaat ctgaaaatcg
     1261 gtacaagcga acggaccgtg ggcgaagatg cgcagagcac cggaggcacg atggacgaaa
     1321 gtggctctcc actaagccct aaggattgca gttggaccgc gacaacaccg ggtgtctgct
     1381 gcatcagttc gatgatttcg ctggcgacga cgttttgccc aggctggcgc gccctgaacg
     1441 agaaagcgtt gctgacgttc tgccacacat cagccagcac aagtggcggc gagtaatcgc
     1501 tgtctacgcg aatctttgcg gtgaacgtaa agagcgttgg tgcatacgaa acaactttca
     1561 acggcacata aggattgccc tgggaacgca gagcttgcac cagatgagtg atgatgtcgt
     1621 catcgggatg aagtaccgcg ccattttctc cggcgaccgt gaggtaaacc ccgcgtgtat
     1681 tgccgaacca tgtccatgtg gccaacgcct ttgctattcc ggcgaaggcg agcgcgtagt
     1741 tctggtagtc ctcgagcgag acgatgcggt tgagtgtaag cgtgggcagc ggcgcgctgg
     1801 tgcgggcgtc atctgatgat gcaggatcag ctgcgccgga agcgggactg gggttcgtcg
     1861 cggacttcag cccctgcgga cggtcgagcg gctgtgaaag ctggcccgcc tgtaccatgc
     1921 ctgcgatccc aatgcctttg cgatagacag cgcgaatatt ggcctgtcct gtcggtgtgc
     1981 gcgaaccgtt ggtgccattt ccaaactgga tgacgacatt tcccgcagcg ttgacgcgcg
     2041 taacgaagac gcgatctgca ggaccggatg acagaaaatt cgggacttca tgccattgaa
     2101 gattgttcac ccagacctca agcgtagact gtgaaccatt tcccgaagat gctgagacat
     2161 acgtcaacgg ggcctgcttc agcgtgaatt caagcgcatc gttcgtagcg tcgccattgc
     2221 ccaggatctc ttgtacggtt tctccgtgcg ttgcatctac tgcattcgcg ttcacattga
     2281 cggtggcacg gtcatagatg cgtcccagag gctgcacaag cgacagcgta gtagtgtctc
     2341 cctggacgct tgtactctga acaattgcgg cctcgcccac tgtgggatca cccgaatcag
     2401 acggaagcag cgtgacgttg cctgtatctg taagcaggga cccgggcacg ccgctcagtg
     2461 tgatgacgct ccagaggtca ttcactggtg gaaagctgtc aatgagaaaa atctgaccat
     2521 cagaaaccgt caatgctccg gcagcatttg caggtgtaaa gattgcgctg gctccagtga
     2581 cgacttgcag gcggacccgc tttcctgaaa ctccgatggg ctggtttgac gcaatcttct
     2641 gaccacccac aatggaaagg gttgttcctt cgacgggagc aagcattccg gattgctttg
     2701 tgtaactgcc gtcgagggcc cagcccgtaa gcggtagagt tgttggcgtc aactggacgc
     2761 tctgaatata tgcagtgatg tcgcgcgtct cttttacaaa ctcccagagg acttcgttca
     2821 acgacagggc cttgtccccg tagatgattt caccgaggtc gagcgtgagt tgtgttgttt
     2881 ttgtggtcag agtgaatgca ttgggattgg attcagacgg ggccgcggtg atttgaaaga
     2941 acgaggtata gccgagtccg gtcagaacga cccattgacc tgcttccaga ccggaatagg
     3001 aggcgtcgag gttgatctgc ccgctgtttt ctttaaatgt ccgagtgaag tcccagtcgg
     3061 taccacctgc aacgtaaccg gtgatcttcg tcgcgtctat accgctgttt tcgccatttg
     3121 ggatcaaggg catggctggc gcctgaacgc catacagcgc ggccttcctg cgaaagacat
     3181 atacgctgac gttgtccaac gcaccttgta attcgctggt gaaggtgttg tcccaggtaa
     3241 tttttgtccg ctttgcatcg ggatcaacga aaacatgagt gacgtaatgg acgtcgccta
     3301 tgtttttcga gggaggcttc cccgccgcgc tgctgacaaa gagcagtgcg tctccaacac
     3361 tgatattgtt ggccgtgccc tcgatccagg tgctggtgtc ggagggtgag agctgccacg
     3421 gccgcgcggt ctgcgctggc aatgcattcc atgcaatgac agcggtgaga tcggccgatg
     3481 tttcaaagac ctgcggcgtc tgtccggggc caggaatgct ctgcacgcgg gtacctgtgg
     3541 gaatgagcac gttgtcgggc gatccgggag cgctgctgag tgtgaaagcg agaatgtcag
     3601 aggccgcaac gccacgtgag ggaacatatc caaccagacg cgccagctca aaaacagaac
     3661 gctggtctac ggcggtgcgc agccacgctt cgtttgcgga ccgctcctga taaaaggtga
     3721 gaatatcgca gacgacagac cacgcatcga gcagagcaat cgtgaagtca gatgagtcac
     3781 gcgtgcgaag cagggcaagc gccggataat caggatcgga aagcgaagcc agcagactcg
     3841 ccctgaagct gctgtaggtc ccggcgcgat aaacaatcgc agacaaagca ggacggttgt
     3901 aaatgacctg cggggtctcg cggcccatgc cctcgcagca gccgcacagt tgttggtcct
     3961 ggatgacgca ctcgccgttc atttgccacc atccatgtgc aaaacaaaaa tgccgtggtc
     4021 cagacggttg ggatcgttgt cacaccgcgc aatctcaagc cgtcccatag tgagttgacc
     4081 ctgtgcgact ccgtcaacgg aaggatcatc catgcgttca aataccgtga gtgttgctga
     4141 cactacacct tctacggatt gcgcggcgga aatcaatgga ctggcgtaga cagtttgccc
     4201 aaaggtaaag tttttcggat tgagcagacc tggctgtcca ctgcactgat ttcccgtgat
     4261 gaacagatgc aggagcgctt cttccacatc gctttggaag tggtctgccg caacacagat
     4321 ttccatctcg atacgcaggc ctacaatctt tgcgccttct gactcgatgt ctacgcccat
     4381 catgcggcgc atattgagcg tgtcgttgac agcggtgata agtgcaggag acattgcttt
     4441 tgctggagaa acagagacaa atgccgtatg ccagcttcct gtccagcgca atgtgccgcg
     4501 cgcttcgcta acacctgcga cggtcatggc ctgggtgccg taatcatctt ccatcacggc
     4561 gcgtaattgc gtctgaaaac tgaaaggagc gtactggcga atgtgttcca tgtcttccgg
     4621 atctgttccg ccggctgcgg cgagcggatt gcgcacgcca gtgattccgg gcacaggcgt
     4681 aacaacatgc gcgagcgtat cgcgtccgat attgccggcg ctgccgttgc ccacgcgata
     4741 taaagcgtaa aaatgctggc cctgttccgg ggccattcca tattggccat cgccgaagcg
     4801 cagaaagacg cttccattgt gctcgatctc cggtacgcag acacgctcgt tctcattgct
     4861 ggaaagcagg tccggaggcg atggaatggt ccagaattgg ccatcgtcgc tttggatctg
     4921 aatctgcggc tgagcagcaa cagccgtagc aggaggcgca agaaatgccg atgcaggagc
     4981 tgaagcatcg aatggcaatg caaaggtgag cggtgaattc tgaagctcgg gataatatct
     5041 tggccgccct gccggcggac caggctgtcc tgagcaactg caactggttg cagggttcgg
     5101 cgcgggaggc gccgcgggga cttcgcgcag gtcctcccag cgatcaagcc agacgccgtg
     5161 gtcaacagga atgatgttgc catgcgctac gctgaccgct ggtacaaaga cggtcttgcc
     5221 gtcagcgtca gtaaacgtcg aagaaatgca aagaggaaat ggcaaagcat cctcctgcgc
     5281 ccactcgatg cgcgtgacag atttcagtgt caacggatcc atcagcacag aactgtcatg
     5341 gatcaggcgc accgcccagc gatgtgttgg gtccgcatcg gcgctgtttc cggtttgagg
     5401 gccagccact tcttcaaaga caagaatcgt cccttttttc aagctcgtga agtttcctgc
     5461 aagtgttgct tctgttgcac caggaggcag gcagcagttc gtatcgctcc acgtatagaa
     5521 ctgcatttcg ttttgttcag ggtagagtgt tgcgtctgcc atgctggcga agatgggctg
     5581 cgtggttttc gcaagctgtt gtgcggcggt gcttccaggt tttgctgatg cgggcaatcc
     5641 aggcacgcga gcgtaaaagt gtgttccctg aggcagggta atgtttgcgg acaccgaaag
     5701 cgcaacccac gcgcgcgcat tacatccttc gttgagatag tagtccacca gacgcgcatg
     5761 ccggcgtaat gagatgcggc tgcgcgccgt gccaatatag gcctccgtgc tgacggcgtc
     5821 ctgctggtag ctg
//
LOCUS       DASRBV010000365         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_334974, whole genome shotgun sequence.
ACCESSION   DASRBV010000365 DASRBV010000000
VERSION     DASRBV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334974"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFA02_13460"
     CDS             complement(<1..932)
                     /locus_tag="VFA02_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10905.1"
                     /translation="MKLTIDNVDYTSALAARPPLRIEHELNAPGICSFVLVPSAANLA
                     TPLRFGRVVVSDNNGTVLFTGYIATEPALELAGEGTAGPVYQAVVSAISDELLLDMQG
                     VPQLMAMYTEPAGQILQSFTATVDAARLSFNTAQATGITGRFVAEPSENWSANAGLLA
                     SHSRNAYRAIAGSVTLAPIGSITHQLSESAGTLQMGHLQASLVKTLANDVTVCGDEEP
                     SAYVTEIFQGDGITTLFELTEGPFFPSSSKAHPLVDLFQGPAVNPGLWQVQDPGQHVS
                     LTANGLTCSGGNGYDGQTFVSAISSFELGGSLMV"
     gene            complement(944..1924)
                     /locus_tag="VFA02_13465"
     CDS             complement(944..1924)
                     /locus_tag="VFA02_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10906.1"
                     /translation="MSTATVTNFMALPVVTRRVRAYFAPVNRTNQTPTIFDPSQNAQF
                     PLDVPPAPWIDLGWIEDFARKSASTFSALNTGVPATTQYQVREAVGATVSFRFKTWNK
                     LTMALACGSQHMNLLQPASGSAANGSGAKAMPAVSVQAGSTPTFIALNAGDTANFSAG
                     ALVAVDVDYAGQTGFVGSGVSAAYLRSAAAVNNDADYIRRVTFNVARVAQVTPGGLTL
                     AQPLIAGTPANAMKAQPILGFVDREGGSFFQEWSALFVMPGEQGERIFFHYPRLQAMQ
                     GAQETNDVLAAPLERVALNASFRALPVTDTNDGEQVVCFRSFLPAPVALV"
     gene            complement(1924..2409)
                     /locus_tag="VFA02_13470"
     CDS             complement(1924..2409)
                     /locus_tag="VFA02_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10907.1"
                     /translation="MQNVKDTFYITLRNRLAALNPQRVVMLRGVQRPGILVEEAEAPM
                     SQLPSDVFVLRWTVLNADTQLPSTLTQMTCEIHYATGGTQTNSGLDRGRAMAEMDAEL
                     LSILQPASAQKMDYTQTPAAQMETLVFWTAPEFSPLATSRERLMRVVKVNVFAFEEQG
                     E"
     gene            complement(2474..2920)
                     /locus_tag="VFA02_13475"
     CDS             complement(2474..2920)
                     /locus_tag="VFA02_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10908.1"
                     /translation="MSDLSVRNPNQSPALMADVLLRAAGGTTVSLLVAPATPDSSDAS
                     QIGVNAPNFQQMTLSPAIFRQVRAAMSEGQLPKYELLLSASAVQQQVGALQLTSADAL
                     FDSAAGVLINDAQYVIESRSVSEALGQVYLYHLLLRESHSLKQTLP"
     gene            complement(2924..3547)
                     /locus_tag="VFA02_13480"
     CDS             complement(2924..3547)
                     /locus_tag="VFA02_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10909.1"
                     /translation="MGYLQPTEYTSYGLAADTTDDWITLASSLMENHCRRASLNPTQY
                     IERLRLTTGVQTVRLSYLPLVTVAPATSPLISIKARYARPRRGQLIYPMQEEIAWAFA
                     LPGSWTQVDPSTVDFVPDTGELIFPMNVYGLPFNEVQVTYTAGLATIPDAVKSACALI
                     VKNAQATPGLNVKRSRVDTLQMDYFSNSLIDATVEELLKPWVANRLG"
     gene            complement(3638..>4534)
                     /locus_tag="VFA02_13485"
     CDS             complement(3638..>4534)
                     /locus_tag="VFA02_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10910.1"
                     /translation="FYQTEITDIVRRRGIFGQRIKQVPATGHPSRFFEETGIPSPGTA
                     GFVDPRNIVAPVVSPTRVERSVPLKAIVAQLNYNLFDIELGTQQKQFAYLQAKDLVDT
                     VEGVLHTHDIALWNGNDTSLSSPTTTQYYGAAAQIAAGGNTVTIGTSQSIVDGLKSTV
                     AQMVANSNFAVRPTAIYANPVLLDLIDREMKSEFNVVLNTREVAGFTVKTLSTQAGEL
                     PLIPDWALSYTGTPGSGSAVLPAYIVSEDMIEYHWLSNPNPRVFQLGVPGSLASQYVV
                     VKFGGVVVKGANYAHYQVLVDR"
BASE COUNT          976 a         1343 c         1270 g          945 t
ORIGIN      
        1 tccaccatca acgatccgcc aagctcgaag ctgctgattg cactgacaaa tgtctgtccg
       61 tcatagccgt tgccgccgga gcaggtaagc ccattcgcgg taagcgatac gtgctggcct
      121 gggtcttgta cttgccacag gcctggattc acggccggtc cctgaaataa atccacaagc
      181 ggatgtgctt ttgaagatga cggaaagaac ggcccttccg taagctcgaa gagcgtggta
      241 ataccgtccc cctgaaagat ttccgtcacg taggcagatg gctcctcatc accacagacg
      301 gtcacgtcgt tcgccagcgt ctttactaac gatgcctgta agtggcccat ctgcaaagtg
      361 cccgcagatt cgctcaactg gtgcgtaatg ctgccaatgg gtgcaagtgt gacgctgccg
      421 gcaattgcgc gatacgcatt gcgcgagtgc gatgccagaa ggccagcatt tgcagaccag
      481 ttctccgatg gttctgcgac aaatcttcct gtaatgcccg tcgcctgcgc tgtgttgaag
      541 gaaagccttg ctgcatccac cgttgcggtg aaggactgta ggatctgccc cgcaggctcg
      601 gtgtacatcg ccatcaactg tggaacgccc tgcatgtcca gcagcagctc gtcactaatg
      661 gctgaaacaa cggcctgata aaccggtccg gcagtccctt ctccggccag ctccagcgca
      721 ggctcagtgg cgatgtatcc ggtaaacagc actgttccgt tgttatcaga cacaacgacc
      781 ctgccaaagc gcagcggcgt cgcgagattc gcagccgatg gcacaagcac aaacgagcag
      841 ataccgggtg cattcaattc gtgctcaatg cgcagcggag gtcttgcggc aagcgcagag
      901 gtgtagtcta cattatcaat cgtcagcttc actcggacct cctttagacc agcgcgacag
      961 gcgctggcaa aaagctgcgg aagcacacca cctgctcgcc atcatttgtg tcggtaacag
     1021 gcagcgcgcg aaagctcgcg ttcagcgcga cacgttccag cggcgctgca agaacatcat
     1081 tcgtctcctg cgctccctgc atggcctgca atcgcggata gtggaagaaa atgcgctcac
     1141 cttgttcgcc gggcatgaca aagagcgcag accactcctg gaaaaagctc ccgccttcgc
     1201 ggtctacaaa gccgaggatg ggctgcgcct tcattgcgtt cgcgggggtg cccgcaatca
     1261 agggctgcgc cagggtgagg ccgcccggcg ttacctgagc aacgcgcgct acgttgaatg
     1321 ttacccggcg gatgtagtcg gcatcgttgt tcaccgccgc tgcgctgcga agatacgctg
     1381 ccgatactcc gctgccgacg aatcccgtct ggcctgcgta gtccacatct actgcaacaa
     1441 gagcacctgc gctgaaattt gctgtatcgc ccgcattcag cgcgatgaac gttggcgtag
     1501 agcctgcctg cacgctgacg gcaggcatcg ccttcgcgcc agatccgttt gcagcactac
     1561 cgcttgcagg ctgaagcagg ttcatgtgct gagatccgca tgcaagagcc atggtgagct
     1621 tgttccacgt cttaaaacgg aaggacacag tggcacccac ggcttcccgc acctgatact
     1681 gcgtggttgc cggaacgccc gtattcaacg cgctgaaagt gctggccgat ttgcgcgcga
     1741 agtcctcgat ccatcccaga tcaatccacg gcgcgggcgg aacgtcgagc ggaaactgcg
     1801 cattctgtga aggatcaaaa atggttggcg tctgatttgt gcggttgaca ggagcaaaat
     1861 atgcgcgtac gcgccgcgtg actacaggca gcgccatgaa atttgtaacc gtcgctgtgc
     1921 tcatcattca ccctgctctt caaatgcgaa gacattcacc tttaccaccc gcataagcct
     1981 ttcgcgcgaa gtcgcgagcg gagaaaattc cggagcagtc cagaagacca gcgtctccat
     2041 ctgcgccgct ggagtttgtg tgtagtccat cttctgcgca gaagctggtt gaaggatgga
     2101 aagcagctcc gcgtccatct cagccatcgc ccgaccgcga tcaaggccgc tatttgtctg
     2161 cgttccgcct gttgcgtagt ggatctcaca ggtcatctgt gtaagcgtgg agggcagttg
     2221 cgtgtctgcg ttgaggactg tccaacgaag cacgaagaca tcgctgggca gctgcgacat
     2281 gggggcctcg gcctcttcca ccagaattcc aggccgctgc acgccgcgca gcatcaccac
     2341 gcgctgcgga ttgagggccg ccagccggtt gcgcagcgtg atgtaaaaag tgtcttttac
     2401 gttttgcatt gatttgtcgt cctgaatgaa ctgcactccg ggtcacgaca tctccaaagg
     2461 gagatggaac ttcttaaggc agagtctgtt tcagcgagtg cgattcacgt aacagtaagt
     2521 gatataaata cacctgcccc agagcttcgg aaacagagcg cgattcaatc acgtattgcg
     2581 cgtcatttat aagcacgccc gccgctgaat caaacaatgc atcagccgac gtaagctgga
     2641 gcgcgcccac ctgctgctgc acggcggatg cagagagcaa cagctcatat ttcggaagct
     2701 gtccttcact catcgccgcg cgcacctgac ggaagatcgc gggagacaga gtcatctgtt
     2761 ggaaattggg cgcgttcaca cctatctggc tggcatcgct gctgtcgggt gtggctggag
     2821 caaccagcaa tgaaacagtc gtaccacctg ctgctcgcag caacacatcg gccatcaatg
     2881 ccggactctg gttcgggttt cttacgctga gatcactcat ggctcaccct aaccggttgg
     2941 cgacccacgg tttcagcagc tcctcgaccg tggcgtcaat taatgagttg gagaagtagt
     3001 ccatctgcag agtgtccacg cggctgcgct ttacgttcag tcccggagtg gcctgtgcgt
     3061 tcttcacaat cagcgcacac gcggacttca ctgcatcggg aatcgttgcc agccctgccg
     3121 tgtatgtcac ctgcacttca ttgaagggaa gcccgtacac attcatcgga aagatcaact
     3181 cgcccgtatc tggaacaaaa tccacagtag atgggtccac ctgcgtccag cttccaggca
     3241 gcgcaaaggc ccaggcaatc tcttcctgca tgggataaat caactgcccg cgccggggac
     3301 gcgcgtaccg ggccttaatg ctgatcaaag gagatgttgc cggggccacc gtgaccagcg
     3361 gcagataact cagccgcacg gtctgcacgc ctgttgtcag ccgcagcctc tctatatatt
     3421 gtgttggatt caggcttgcg cgcctgcaat ggttctccat cagcgaggat gcaagcgtga
     3481 tccagtcatc ggtcgtgtca gccgcaagcc cgtagcttgt gtactctgtc ggttgtaaat
     3541 aacccattgt gtctccgcgc tgccggagtg ccgcagcaaa tgttttgcaa aaaaaagccg
     3601 gccccgtcat ccagggccag ctctccagga agaaggatta gcggtctacg agcacctgat
     3661 aatgcgcata gttcgcgccc ttcactacaa caccgccaaa cttcaccacg acatactgcg
     3721 aagccagcga gccaggtacg ccgagttgga agacgcgcgg attcgggttg ctcagccagt
     3781 ggtactcgat catgtcctcg ctcacgatgt aggcgggaag cacagccgag cccgacccgg
     3841 gcgtgcccgt gtagctcagc gcccagtctg gaatcagcgg cagttcgccg gcctgcgtgg
     3901 agagcgtctt gacggtgaag cccgcgacct cgcgcgtgtt cagcaccaca ttgaactcgg
     3961 acttcatctc gcggtcaatc agatcaagca gcaccggatt ggcatagatg gctgttggac
     4021 gcacagcaaa gttgctgttc gccaccatct gcgcaacggt agacttgagg ccatccacta
     4081 tgctttgcga ggtgccgatg gtgacggtat tgccgccagc agcaatctgc gccgccgcgc
     4141 cataatattg cgtggtggtc gggctgctca gtgaagtatc attgccattc cacagcgcga
     4201 tatcatgcgt atgcaacaca ccctcgaccg tgtccaccag gtctttggcc tgtagataag
     4261 cgaactgctt ttgctgcgtg cccagctcaa tgtcgaacag gttgtagttc aactgcgcga
     4321 caatggcctt gagcgggaca ctgcgctcta cgcgcgtggg gcttaccact ggggccacga
     4381 tgttgcgcgg gtccacgaag cctgcggttc cgggagaagg aatacccgtc tcttcaaaga
     4441 agcgcgatgg atgcccagtc gccggcacct gcttaatgcg ctggccaaag atgccgcgcc
     4501 gccgcacgat gtcggtaatc tcagtctggt aaaa
//
LOCUS       DASRBV010000366        15376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_434678, whole genome shotgun sequence.
ACCESSION   DASRBV010000366 DASRBV010000000
VERSION     DASRBV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 15376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15376
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434678"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2574
                     /locus_tag="VFA02_13490"
     CDS             124..2574
                     /locus_tag="VFA02_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZU10911.1"
                     /translation="MKHRTIKHYELVRRLGAGGSGVVFLANDTLLMRPVVLKILKRGA
                     LTLEQMRTTVLREARMASAIEHPNVCAIYEVGEEGEEAFIVMQYVPGQSLDKIIAKGP
                     ASIQLVLSVGIQIADGLNAAHSLGIFHRDLKPANAILTDGGLVKLLDFGLARRISLEE
                     AEFDPAKSSRRKTGPVAATYTARGGTIAYMAPEQFVTGQSSVQSDIWALGVILYELVS
                     GRHPFARPDADEFQSIRAIQFVDPPPLSQCCPEVPPELGSVIMRCLEKNPAERYASAA
                     EVREALKTIMKALQIETGILPGEVTAQLPTPEEEKRTTGLLSMLAERFRESADTQARQ
                     NTIVVLPFRNLGPSEVAPLYGFALADAISARISRMSSLVVRPTSALMHLPLAQMDPLE
                     IGQKLLVRWVLTGNFIRSEKGFDLNWQLLDVSRQSVSSGGSITVPSFDLVAVQTEICN
                     EVFASLQGMGQFVPTSERQTPRTTLTEEISEEYLQARAVLSAFMQRTGSKGDLDHARE
                     LFERVTQSNPSFPPAWSGLGITHLQYVRHGFGGHMHVMSARKAFEQALQLDPGQVEAN
                     LYRVYMLLSRGEKESARHGIEHLLRTASNDWNVHMVAGMTLRLDGMYEEALDQFNQSL
                     KLNPSNAPILYNHRARVYQYQNQLELAEDELEKGLTLEPKHPLLRTSLAYQRMRQGDL
                     RQAIAMLEAVIRDDESMRIAFPTLAMCYVQVGERERAASLITEDSISAAEADSEMAYR
                     LATYFAVEGDESEALHWLRRAIYLGNENYPWFSKNPAWNRLHGHTDFERILEDLKKSF
                     RRNQKTWKRLLAQVPKVV"
     gene            complement(2665..5142)
                     /locus_tag="VFA02_13495"
     CDS             complement(2665..5142)
                     /locus_tag="VFA02_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10912.1"
                     /translation="MTNFLSHLRYSVRHLRKTPGFTIVCVLTLALGIGANTAVFSVMN
                     AVLLKSLPVADPQKLVYLFHTGFPDQANNTGDSNTSFSFPTYNTLRRNHPGFSDVIAY
                     VPMSFDGNVAVRFGTNPDTAEGDMVSGNFFSGLGVRPARGRGFTAQDENQHAPVAVIS
                     YGYWTRRFSRDPDVIGKTLFIKSTPFTIVGVTPEGFEGTEAGKSTDFWIPLQSRPEFN
                     AWGVPPINGKIYLDRPNWWCLHLIARIAPGVSKSRALEQIQPLFQSAAYIGIGTPKKG
                     EKPPVLSLVDTRGFPGADEYLGRPLKTLMAMVSLVLLIALSNVAMLLMARNAVRQREF
                     SLRLALGAGRGELFRQLLTEGLLLISIGGLLAWLFAEAASRALGKWAQIESSVAPDTT
                     VLLVTLMVLVISALLFALAPLRIALEGGPAHALKTTSSMANTDAGRARTGKIIVALQM
                     ALCVVLLVGAGLLVRTLRNLQNTPLGMRTEGLVVFGVNPQHVHSLAESRAFYEDLTRR
                     LRVLPGVESVGLISMRPGSGWSNNYPIYVDGKQPDVNGGQVRNNAVGPDTFHTLGVPV
                     IAGREFKDSDTASAPRVAIINETFAKMYLPNQNPIGHRLGGSKPDEQATIIGVVKDHK
                     FTGITETPTPTYWDNYLQKDRLGQMHVELRVHGDPLAVLPAARKALQQMDPDLPLMNP
                     QTQQAQFEESISQQVLFARLGGFFGLLAVVLVATGLYGTLAYRVNNRTVEIGVRMAVG
                     AKRGQIVWMVLRDSLVLTAIGLVLGIPLAAFVTKALSSALYGVKVRDVTSYMLAVAGV
                     VLVGFIASALPARKAANVDPLNALRAE"
     gene            complement(5399..6955)
                     /locus_tag="VFA02_13500"
     CDS             complement(5399..6955)
                     /locus_tag="VFA02_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HZU10913.1"
                     /translation="MAIFAAATYFSVLAPSLPAQSLDPSAILHPAPDTWPTYHGDYSG
                     RRHSALTQITPQNVGNLTLAWVFQTDQNAAIKSSPLLVDGILYFTVPDNIWAIDARSG
                     HMLWHYNKPTEEGDHIGQRGVAMYKGWLYFLSPDGHLTCLNAKNGSVRWTVQVADVSK
                     GYWTTMAPLVVRNHVIIGVSGDMDNLMGYLRSIDPETGETQWQWNSTPPAGTPNSTTG
                     GMTWITGTYDPELNLLYWGTGNPTPVLNGKPRPGDDLYTCSIVALDPDTGKLAWGFSV
                     SPHDTHDWDAVETPVLVDGDFHGKPHKMLMQASRNGYFFVLDRTNGKSLLTVPFGPVN
                     WSLGVDKDGHPIPNPEKEPAPDGRLIAPDEGGLTNFRSPSFDPKTGLFIVDAHPSYSI
                     YFAKPADGSYGWAGADYGLWGKGVIEAIDYQTGKIRWSHDVGSGGSGAGVLTTDSGLT
                     FTGDAHGNVLALDTRTGKTLWHAGAGAPMYSSPISYELDGRQYIMTSSGGVLFAWALP
                     EAYSKQSPAR"
     gene            complement(6994..8016)
                     /locus_tag="VFA02_13505"
     CDS             complement(6994..8016)
                     /locus_tag="VFA02_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU10914.1"
                     /translation="MKKLCTVTLLVAAVWSISSATGARAQAGNAEGAQSYAKHCAICH
                     GDHREGDLPGFPPLLDVSRQMNDQQIATLIQTGKGRMPAFPKLPQQELTALLRFLHSD
                     DLSAAAQGSGGASNSAEAGRHLFQRNCAFCHGRDAAGGETGPDLTRSKLALADVNGDK
                     IGEVVRNGRPEKKMPAFNFSVDEIHSIAAFIHYQQKKAAAHPGGRRGVDVSDLQTGNA
                     SAGKQYFFGAGGCSRCHSPTGDLAGIATRLEGLQLEERMLYPRDARSDVTVTLPSGEK
                     ISGVLAYRDEFTIGLRDNSGTYHSWPTSRVRYTVDTPAEAHVELFSKYTDEDIHNLMA
                     YLQTLR"
     gene            complement(8033..9427)
                     /locus_tag="VFA02_13510"
     CDS             complement(8033..9427)
                     /locus_tag="VFA02_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZU10915.1"
                     /translation="MNRRQFLSSAGVLFADKSLHALAPRSFAPAAGAAKADYSLSIRP
                     CTLEIGPGVRVKTIAYNGQVPGPTLRLREGVPVTIDVTNASENADIVHWHGLAIDSYN
                     DGAMEEGSPMIAAGHTQRYSFTPRPSGTRWYHSHASANDNLAMSTYTGQFGFLLIEGR
                     EQPAHYDQEINLAIHHWEPSFVPMVEMMRSASSNMPLTTGSDVGYKYATINSHMLGAG
                     EPIRVKQGQRVLFRLLNASATENIVLALPGHTFKVIAMDGNPVPNPKPVEVLSLAVAE
                     RIDAIVEMNSPGVWILGSTLEKARQMGLGIVIEYAGKKGAPVWKDPAPVEWDYTQFAD
                     ATVAPKPDETFTLTFRDIGPLHGSKFDTWTINDKSWPDIEPLIVQKGKRYRLVLRNGS
                     GDQHPIHLHRHTFEVTRIGDRQISGLKKDVINVMPLDAVEVDFIADNPGDTLWHCHQQ
                     LHMDYGFMQLIKYA"
     gene            complement(9465..10424)
                     /locus_tag="VFA02_13515"
     CDS             complement(9465..10424)
                     /locus_tag="VFA02_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU10916.1"
                     /translation="MRLGVFTPLLSQMPLEDALAKLKSFGISTVELGTGNYPGDAHCK
                     LSMLDDPAALRDFKKKLDDHGFSISALSCHGNPLHPGPSIAQNAQEVNRKTILLAEKL
                     GVSVVVDFSGCPGDSPNAKAPNWVTCPWPPEYLDILNWQWEKVVTPYWMERAKFAADH
                     GVRIAIEMHPGFVVYSPETLLRLREIAGPSVGCNYDPSHMFWQNIDPIAAIRVLGDAI
                     FHVHAKDTQLYPANLLRTGVLDTKPYTDERNRGWVFRTCGYGHGAEWWKEFVSTLRMF
                     GYDGVLSIEHEDSLLSAEEGLSKAAQFLNDIILKETPGTAWWV"
     gene            complement(10438..11598)
                     /locus_tag="VFA02_13520"
     CDS             complement(10438..11598)
                     /locus_tag="VFA02_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU10917.1"
                     /translation="MKKIGMGLVGPGFVAAHHLDAVRRLGDVEVIAIAGSSQESAEKK
                     ARAYKVDRAYGDYKALIADPDIQVVHNTTPNHLHLPVIMAALQAGKHVISDKPLALNA
                     AEGRKLRDAALTAKVAHIVTFNYRGNPLVQQARGMIARGETGPLSFIHGFYLQDWMTD
                     PNVYSWRSDPAKGGVTSALGDIGSHWCDIAEHISGLKITAVLADLTTVIPVRYSSGAS
                     AEAFSSTNEGERRPVQVQAEDLASVLLRFENGAKGCFTVGQVLPGHKNDLQIELNGRA
                     RSLKWRQEDQNELWIGRHNQPNCIMAKDPSLVSPDALPYVHLPGGHQEAWADAFRNLI
                     ADAYQWIREGGRPEAKPAPLPTFEDGYRSTCLIEAMLKSHAAGGVWTKVEQH"
     gene            complement(11617..12495)
                     /locus_tag="VFA02_13525"
     CDS             complement(11617..12495)
                     /locus_tag="VFA02_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZU10918.1"
                     /translation="MRHPRKFACSLVAFLFFGALSLTSPAQQSNKDLYLPAAPHAKQI
                     HLKHVLVIGQTKGFEHDSVSAAMNAIYEMGKESGLWDTEMRTDTELLTKKELGRNAKN
                     LNYFDVLVFASTTGELDMDADQKRDMMSFIKEDGKGFVGIHAALDTNYTWPEYGEMIG
                     GWFDQHPWMTFNAPIINEAPDFPAVRHFPTEFVKYDEIYQPKDWSRDKVNVLLSLDPT
                     KLNYENNPRIHRTDHDFAVAWCKMYGKGRVFYSTLGHTEEAWEDPDIRKMYFEAIKWA
                     MGLTEGSTTPHPRPTR"
     gene            complement(12600..13442)
                     /locus_tag="VFA02_13530"
     CDS             complement(12600..13442)
                     /locus_tag="VFA02_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU10919.1"
                     /translation="MPDRRTFLKSGLAAAIAAKAPLAHATQRKGRIHQSVCRWCYQHV
                     PLDQLCAYAAQIGLKGIDLLEKNDWEVPRRYGLICTMAYAGGGTISDALNRTENHDAI
                     ERAFRENISVAAKAGIPNVITFSGNRRGMSDEEGARNTIAGLNRVKKIAEDHGVTICL
                     ELLNSKRNHPDYMADHTAWGARVIHEVNSPRVKLLYDIYHMQIMEGDLIATIRENIGA
                     IAHFHTGGIPGRHEIDDTQEIQYAAVMRAIADLDFSGYVAHEFLPQRDPFTSLREAVD
                     LCDV"
     gene            14436..>15376
                     /locus_tag="VFA02_13535"
     CDS             14436..>15376
                     /locus_tag="VFA02_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10920.1"
                     /translation="MQSASDIFGQVFRAIWANKLRSFLTMFGIAWGVGSMLLLVGVGE
                     GFRSGQRRELATLGNDLIMMWGGTIPAVPNQHTGMRPYNLTLSDAEAIRGEASEVRAV
                     TAFINRNDIKQQSLYESAGGQVIGAEPNYSGVRFLPVKEGRFLRDADMRDRRRVVVLG
                     EKSAKLLFPGRPALDETVLLNGARFQVIGVAGKTGRGNNDTENQKIYIPLSTMLEIFP
                     VKGDNIPADSVTSIQYQPRVRGENAAAKQQVHEIIARRHGFDPNAQDAFNEWDTIKTE
                     QMVGKIFTAMDVFLGGVGIVTLALGAVGIINIMLVSVT"
BASE COUNT         3403 a         4191 c         4253 g         3529 t
ORIGIN      
        1 gaaggacttg gcatggaggt ctggatgaca aaatgatttc acttccggtt ttgggacacc
       61 gcattcgacg caccagttgt tctttatgct tttgcaatgc tgcgcccgct acactaacaa
      121 tagatgaagc atcgcacaat caaacattat gagctggtaa ggcggctggg cgcgggtggc
      181 agcggcgtcg tatttcttgc caatgacacg ctgctgatgc ggcccgtggt gctgaaaatc
      241 ctgaagcgcg gcgcgctcac gctcgagcag atgcgcacaa ccgtgctgcg cgaggcgcgg
      301 atggcttcgg ccattgagca tccaaatgtc tgcgccattt acgaagtggg cgaggagggc
      361 gaagaggcct tcatcgtcat gcaatatgtg cctgggcagt cgctggacaa gatcattgcc
      421 aaaggtccgg ccagtattca gctcgtgctt tctgttggca tccagattgc cgatggtctg
      481 aatgctgcgc attcgctggg catttttcac cgcgacctga agccggccaa tgccattctg
      541 acggatggcg gcctggtgaa gctgctggat tttggcctgg cgcggcgtat cagtctggag
      601 gaagcggagt ttgatcccgc gaaatcttcg cggcgcaaga ccgggccggt ggctgccact
      661 tatacggcgc gcggcggcac catcgcctac atggccccgg agcagtttgt gaccgggcag
      721 agcagcgtac agagtgacat ctgggcgctg ggcgtcattt tgtatgaact ggtcagcgga
      781 cggcatccat ttgcgcgtcc tgatgcggat gagttccaga gcattcgcgc catccagttt
      841 gtggacccac cgccgctctc gcagtgctgc ccggaggtgc ctccggagct tggctcggtc
      901 atcatgcgct gcctggagaa gaacccggca gagcgatatg ccagcgcggc agaagtccgc
      961 gaagcgctga agaccatcat gaaggcgctg cagattgaga cagggattct tcccggcgaa
     1021 gtgaccgcgc aactacctac tccggaggaa gagaaacgca caaccgggtt gctttccatg
     1081 ctggctgagc gcttccgtga gtccgctgac acgcaggccc ggcagaacac catcgtagtg
     1141 ctgccattca ggaaccttgg cccgtctgaa gtagctcctc tgtatggctt cgcgctggcc
     1201 gatgcgattt ccgcgcgcat ctcgcgcatg tcgtcgctgg tggtgcggcc cacgagcgcg
     1261 ctcatgcacc tgccgctggc acagatggac ccgcttgaaa tcggacagaa actgctggtg
     1321 cgctgggtcc tgaccgggaa ctttatccgc tcagaaaagg ggtttgacct gaactggcaa
     1381 ttgctggatg tctcgcggca aagcgtaagc agtggaggct ccatcaccgt gccctcattt
     1441 gatcttgtgg cggtacagac ggaaatctgc aatgaagtat tcgcttcgct ccagggaatg
     1501 ggccagtttg tgcccaccag tgaacggcag actccgcgca cgacgctgac ggaggaaatc
     1561 tcggaggaat atctccaggc gcgcgccgta ctttctgcct ttatgcagcg cacgggcagc
     1621 aagggcgatc tggaccacgc gcgcgaattg tttgaacgcg taacccaaag caatccctcg
     1681 tttcctccgg cgtggagtgg cctgggcatt acgcatctgc aatatgtgcg gcatggattt
     1741 ggcggacaca tgcacgtgat gtctgcgcgc aaggcctttg agcaggcgct gcagcttgat
     1801 cccggacagg tcgaggccaa tctgtatcgc gtttatatgc tgttgtcgcg cggcgaaaag
     1861 gagagcgcgc ggcacggcat cgagcacctg ttgcgcaccg cctcaaatga ctggaacgtc
     1921 cacatggttg cgggcatgac gctcaggctc gatggaatgt atgaagaagc actggaccag
     1981 ttcaaccagt ctctgaagct gaatccatcg aatgccccga ttctctataa ccaccgtgcc
     2041 cgtgtctatc aatatcaaaa ccagcttgag cttgcagaag acgaactcga aaaagggttg
     2101 acgctggagc cgaagcatcc gctgctgcgc acctcgcttg cgtatcagag gatgcgccag
     2161 ggcgacctgc gtcaggccat tgcgatgctg gaagcagtca tccgcgatga cgagagcatg
     2221 cggatcgctt ttcccacact ggccatgtgc tatgtgcagg tgggtgagcg ggagcgtgct
     2281 gcttcactga ttaccgaaga ttctatttcc gcagccgagg ccgacagcga gatggcctac
     2341 cgccttgcta cgtacttcgc tgttgaaggc gatgagagcg aggcccttca ctggctgcgg
     2401 cgtgcgattt atctgggaaa tgaaaactat ccgtggttct caaagaaccc tgcctggaac
     2461 cggctccatg gccataccga tttcgaacgc atacttgaag acctgaagaa gagcttccgc
     2521 cgtaaccaga agacatggaa gcgcctgctg gcccaggtac cgaaggtggt gtaataaagg
     2581 gcctgtatct ccggcatgca atttgcctga gtgtctatcc tgtcgcgctg cttttgcgca
     2641 acaggatggg atactcacaa ctcttcattc cgcccgcagt gcgttcagcg ggtccacatt
     2701 tgctgccttt cttgcgggaa gggcactcgc tatgaacccc accaggacca ctccggcaac
     2761 ggccagcatg tagctcgtta catcgcggac tttcactcca tacagcgcag aagacaatgc
     2821 cttcgtcaca aaggcggcca acggaatgcc gaggaccagt ccgatggcag tcagtaccag
     2881 gctgtcgcgc aacaccatcc agacaatctg cccgcgcttt gcgccaacgg ccatgcgcac
     2941 gccgatctcc acggtgcggt tgttcacacg gtaggccagc gttccgtaga gtccggtggc
     3001 caccagcacg actgcgagca ggccaaagaa ccctcccaac cgcgcaaaaa gcacctgctg
     3061 cgatatggat tcttcaaact gcgcctgctg cgtttgtggg ttcatgagcg gcaggtcggg
     3121 gtccatctgc tgaagcgctt tgcgcgcggc gggcaggacg gccagcgggt ctccgtgtac
     3181 gcgaagttcc acgtgcattt ggcccaggcg gtctttctgg agatagttgt cccagtaggt
     3241 gggagtgggt gtttctgtta tgccagtgaa cttgtggtct ttgaccacgc caatgatggt
     3301 cgcctgttca tctggtttcg agccgcccag ccggtgtcca atgggattct gattgggcag
     3361 atacattttt gcaaacgttt cattgatgat ggcgacccgc ggagctgagg cagtatcgga
     3421 gtctttgaac tcgcgtccgg cgatgaccgg cacgcccaat gtatgaaatg tgtccggtcc
     3481 aacggcgttg ttgcgcacct gtccgccatt cacatcgggc tgttttccgt ccacatagat
     3541 gggataattg tttgaccagc cagaccccgg acgcatactt atcaacccga cggactccac
     3601 cccgggcagc acacgcaggc ggcgtgtcag atcctcatag aaggccctgc tttcagccag
     3661 agagtgcaca tgctgtgggt tgactccgaa gaccaccagc ccctccgtgc gcattccgag
     3721 cggagtgttc tgcagattgc gcaaagtgcg caccagcagt cctgccccga ccagcagcac
     3781 tacgcagagc gccatttgca gagcaacgat gattttgcct gtgcgcgccc tcccggcatc
     3841 tgtatttgcc atagaggaag tggtcttcag cgcatgggca ggtccgccct caagcgcaat
     3901 gcgcagaggc gccagcgcaa acagcagggc cgagatgacc agcaccatca gtgtcaccag
     3961 caggaccgtc gtgtccggag cgacactgga ctcaatctgc gcccacttgc ccagcgcccg
     4021 ggaagcggcc tcagcaaaga gccatgccag aagaccaccg atggagatca acaaaagccc
     4081 ttctgtcagc agttgacgaa acagttctcc ccgccctgct cccagcgcaa gccgcaggct
     4141 gaattcgcgc tgacgcacgg cattgcgggc catcagcagc atggcgacgt tgctcagcgc
     4201 aatcagcagg accaggctga ccattgccat cagggtcttc agtggtctgc cgagatattc
     4261 atccgcaccc ggaaaacccc tcgtgtccac cagactcagc accggcggct tctcgccttt
     4321 cttcggcgtg ccgatgccga tataggccgc gctctgaaaa agaggctgaa tttgctcaag
     4381 ggcgcgtgac ttgctcacac ccggggcgat tcgcgcaatc agatgcagac accaccagtt
     4441 cgggcggtcc agatagattt tgccattgat cggcggaacg ccccacgcat tgaattctgg
     4501 ccgcgattgc agcggaatcc agaaatctgt tgatttgccc gcttccgttc cctcaaagcc
     4561 ttccggggta actcccacga tggtgaaagg tgtacttttg atgaaaagcg tttttccaat
     4621 cacgtctgga tcgcgcgaga agcggcgtgt ccagtagccg tagctgatga cagcaacagg
     4681 agcgtgctga ttttcgtcct gcgccgtaaa accgcgtccg cgggcgggcc gcacccccag
     4741 ccctgagaag aaattgccgc tcaccatgtc gccttcggcc gtgtcaggat tggtgccgaa
     4801 gcgtacagcg acatttccat caaaactcat cggaacgtat gcgatgacgt cagagaatcc
     4861 gggatggttc ctgcgcagcg tgttgtaagt aggaaaggaa aatgaggtgt tggaatcgcc
     4921 cgtgttgttg gcctggtccg gaaaacccgt atggaacaaa tacaccagct tctgcgggtc
     4981 cgccaccggc aacgatttga gcagcactgc attcatcacg ctgaagacgg ccgtattcgc
     5041 gcctatgccc agcgccagcg tgagcacaca gacaattgtg aatccggggg tttttctcag
     5101 atgtcgaacg ctgtaccgca gatgactcag gaagttcgtc atgcaatcgc ctctactgga
     5161 gagcttgcaa cttctgggcc attatcaagc gtagctatat cgttgaaatt aaaggttgag
     5221 ggcatcactc tgactggaga gaggtgtccg caaccgggcg gtcgtgtcca gtttcggaca
     5281 gtctggagaa cgaaaaccgc aatcagccct ggcccagatg ccagagattg tgtagtcgca
     5341 tgattcatcc tccatgcatt ccaccgcctg atgggttcga ggtcagctca ttgcgatttc
     5401 agcgcgccgg cgactgctta gagtaggctt caggcaatgc ccaggcaaag aggacgcctc
     5461 cgctgctggt cataatgtat tgacgtccat ctaattcata gctgatgggg gagctataca
     5521 tcggtgctcc cgcgccggca tgccataaag ttttaccggt ccgggtgtcg agcgcgagca
     5581 cgttgccgtg agcatctcct gtgaatgtca gcccggaatc ggtagtaaga acgcccgcgc
     5641 ctgagcctcc agagcctaca tcgtggctcc agcggatttt accggtttga taatcaatcg
     5701 cttcgatcac gcctttgccc cataacccgt aatcggcgcc ggcccatcca taactgccat
     5761 cggcgggttt tgcgaaatag atgctgtagc tcggatgagc atccacaata aacaagccgg
     5821 ttttaggatc aaagctgggc gagcggaaat tcgtgagacc accttcgtcc ggcgcgataa
     5881 ggcgtccatc cggcgctggt tctttttctg gattaggaat gggatgtcca tctttatcta
     5941 cgcccaggga ccagttcact ggcccaaagg gtaccgtcaa caggcttttt ccattggtgc
     6001 ggtccagaac aaagaaatag ccattgcgag aggcctgcat cagcatcttg tgcggcttgc
     6061 cgtgaaagtc gccatccacc agaacaggag tttctactgc gtcccaatcg tgggtatcgt
     6121 ggggagaaac agagaagccc cacgccagtt ttcctgtatc tggatcaagc gccacaatac
     6181 tgcatgtgta aagatcatct ccgggacgcg gttttccgtt caggactggg gtgggatttc
     6241 ctgtgcccca atacagaagg ttcagttcag gatcatacgt tccggtaatc cacgtcatgc
     6301 cacccgtagt gctgtttgga gtgccggcag gaggggtact gttccattgc cattgggtct
     6361 ctccggtttc aggatcaatg gaacgcagat aacccatgag gttgtccata tcgccggaga
     6421 cgccaatgat cacgtggttc cggaccacca gcggcgccat agtagtccag tagcccttgc
     6481 ttacatctgc cacttgcaca gtccaacgca cggaaccgtt tttagcgttc agacaggtca
     6541 gatgcccatc tggtgacaga aaatagagcc atcccttata catggccacg ccgcgctgtc
     6601 caatatgatc accttcttct gtgggcttgt tatagtgcca aagcatgtgt ccggaacgcg
     6661 catctatggc ccagatatta tctggaacag tgaaatagag aatgccgtct accagcagag
     6721 gtgaagattt aatggctgca ttctggtcag tctgaaagac ccatgcgaga gtaagattgc
     6781 caacattttg tggtgtgatt tgcgtaagcg cactgtgccg tcttcctgag tagtctccat
     6841 gataagtggg ccaggtgtcc ggcgcaggat ggagaatcgc agaaggatcc aggctctgcg
     6901 ccggcagaga tggcgccagg acagagaaat acgtcgctgc tgcaaaaata gccaaagtgc
     6961 gtgcaaaaaa cttcatcgta aactgtgacc tcatcagcgc agggtttgca gatatgccat
     7021 caggttatgg atgtcttcat ctgtgtattt gctgaataat tcgacgtgcg cctctgcagg
     7081 cgtatccacc gtatagcgga cgcgactagt aggccaggaa tgatatgtgc cactgttgtc
     7141 ccgcaatcca atcgtgaact catcgcggta ggcaagtact ccgctgatct tctctccaga
     7201 gggcagcgtg acggtcacat cgctcctggc atcgcgcgga tacagcatgc gttcttcgag
     7261 ttgaaggcct tccagccggg tagcaatgcc tgccagatcg cccgtgggtg aatggcaccg
     7321 ggagcagccg ccggccccga aaaagtactg cttgccggcg gatgcattgc ccgtctgaag
     7381 atcggaaaca tctacaccgc ggcggccccc gggatgggcc gctgccttct tttgctggta
     7441 gtggataaag gccgctatgc tatggatttc atcaacggaa aaattaaatg ccggcatctt
     7501 tttttccgga cgcccattgc ggacgacctc accaatcttg tctccgttca catcggccag
     7561 cgccagtttg gagcgcgtaa gatcaggccc tgtttctccg ccggccgcat ctcttccatg
     7621 gcagaaggcg cagttgcgct gaaaaaggtg cctgcctgct tccgcactgt tcgatgctcc
     7681 accagaccct tgtgctgctg cggaaagatc gtcgctatgc aagaagcgga gcagggccgt
     7741 aagttcctgt tgcggcagtt ttgggaatgc gggcattctt cctttgcccg tttgaatgag
     7801 cgtcgcaatc tgctggtcat tcatctgccg gctgacatcg agcaaaggcg ggaaccccgg
     7861 caggtcgcct tcacggtggt ctccatgaca gatggcgcaa tgtttggcat agctctgcgc
     7921 gccttctgca ttgccggctt gtgctcgcgc gccggttgct gaagagatag accacacagc
     7981 agcgaccagc agagtgactg tacagagttt cttcattctt ccaacagcta ccttatgcgt
     8041 atttgatcaa ctgcatgaag ccataatcca tgtgcagttg ttgatggcaa tgccagagcg
     8101 tatcgcccgg attatcagcg ataaaatcta cttccactgc gtcgagcggc ataacattga
     8161 tcacatcttt tttcagaccg ctgatctgcc tgtcgccgat acgggtaact tcaaaggtat
     8221 ggcggtgcag atgaatggga tgctggtcac cgcttccatt gcgtaggacg agccggtaac
     8281 gcttgccttt ctgcacgatg agcggttcga tgtctggcca cgatttatcg ttgatcgtcc
     8341 atgtatcgaa ttttgatcca tgaagcgggc caatgtcccg gaaggtcagc gtaaaagtct
     8401 cgtccggttt tggcgcaact gtagcgtccg cgaattgcgt atagtcccat tcaaccggag
     8461 cggggtcttt ccacaccgga gcacctttct ttcccgcata ttcaatgacg atgcccagtc
     8521 ccatctgacg cgctttttca agcgtggatc ccagaatcca tacaccggga gaattcatct
     8581 ccacaatcgc atctatgcgc tcggccacag cgagcgaaag gacctcgaca ggcttcggat
     8641 ttggcactgg attgccatcc attgcgatga ctttgaatgt gtgcccgggc agtgcaagaa
     8701 caatattctc tgtcgcgctg gcgttcagca atcgaaagag cacgcgctgg ccttgtttta
     8761 cgcgaatcgg ctcacctgcg ccaagcatat gcgagttgat cgtggcgtac ttgtatccga
     8821 cgtctgagcc agtggtcagc ggcatgttag acgatgctga gcgcatcatt tccaccattg
     8881 gaacaaacga tggctcccaa tgatgaatgg ccagattgat ttcctgatca taatgggcag
     8941 gttgttcgcg gccttctatc agcagaaaac caaactggcc ggtatatgtg ctcatcgcca
     9001 gattgtcatt ggcgctggca tggctgtgat accagcgcgt ccccgatggc cgcggcgtga
     9061 aggaataccg ctgcgtatgg ccagccgcaa tcatcggcga gccttcttcc atcgctccat
     9121 cgttgtagga atcaatggcg aggccgtgcc agtggacgat gtcggcattt tctgaagcat
     9181 tggttacgtc tatcgtgacc ggaacacctt cgcgcaaacg gagtgttggt cctgggacct
     9241 gtccgttata agcaatcgtt tttacccgca cacctgggcc aatttcaagc gtgcaaggcc
     9301 tgatactaag gctgtaatcg gcctttgctg ctccggcagc aggcgcaaag gagcgtgggg
     9361 ccagggcatg aagagacttg tctgcgaaca gaactcctgc tgatgaaaga aattgtcttc
     9421 gattcatgat gtagaaagcg ctttcatttc aacgtatgcc gggcttagac ccaccacgcg
     9481 gtcccgggtg tttccttgag aataatgtca ttcaaaaact gtgctgcttt ggaaagtccc
     9541 tcttcggcag aaagcagact gtcttcgtgt tcgatcgaga gcactccgtc gtagccgaac
     9601 atgcgcagcg tcgaaacaaa ttccttccac cactccgcgc catgaccata accgcaggtg
     9661 cgaaataccc atccacggtt gcgctcatct gtataaggct tagtgtccag aactccagtg
     9721 cgcagaaggt tcgctggata gagttgcgtg tcctttgcgt gcacatggaa aatcgcatcg
     9781 cccaaaacac gtatcgcggc gatgggatca atgttctgcc agaacatatg gcttgggtca
     9841 taattgcagc caacggacgg cccggcaatt tcgcgcagcc gcagcaacgt ctccggacta
     9901 tagacaacaa aacccggatg catttcaatg gcgatcctca cgccatggtc cgcagcaaac
     9961 tttgcgcgct ccatccaata aggtgtgacc accttttccc attgccagtt caaaatgtcc
    10021 agatattctg gcggccaggg gcaggttacc cagtttgggg cttttgcgtt gggcgaatcg
    10081 cccgggcagc cagagaaatc taccacaaca gaaacgccca gcttttctgc caaaaggatg
    10141 gttttgcggt tgacctcctg cgcattttgc gcgatggatg gaccaggatg cagcgggttt
    10201 ccatggcagc ttaacgcgct gatggaaaaa ccatgatcat ccagcttctt tttgaagtct
    10261 cgcaatgccg caggatcatc cagcatggaa agcttgcagt gcgcatcgcc gggataattg
    10321 ccggtgccaa gctccactgt gctgatgccg aatgatttga gctttgccag cgcatcttcc
    10381 agcggcatct gtgaaagcag aggcgtaaat actccaagtc tcatcgttat ctgctcctta
    10441 gtgctgctcc accttcgtcc atactccgcc agcggcatgg cttttgagca tggcttctat
    10501 taggcatgta gaacggtagc catcttcaaa agtaggcaga ggagcgggct ttgcttccgg
    10561 acgccctccc tcgcgaatcc actgataggc atcagcaatc agattgcgga atgcatccgc
    10621 ccacgcttcc tggtggcctc cgggaaggtg gacgtaggga agagcatcgg gcgagaccag
    10681 cgatgggtct ttcgccatga tgcagttggg ttgattgtgc cggccaatcc aaagctcgtt
    10741 ctggtcttcc tgcctccact taagcgagcg cgctcttccg ttcaattcga tttgaagatc
    10801 gttcttatgg cccgggagca cctgcccaac agtaaagcat ccctttgcgc cattctcaaa
    10861 acgaagcagc acactggcca ggtcttccgc ctgcacctgg acggggcggc gttctccttc
    10921 gttggtagag gaaaatgctt ctgcggatgc gccggaggaa tagcggacag gaatcacagt
    10981 cgtgagatca gcgagtacgg cagtgatttt caacccggaa atgtgttctg ctatgtcgca
    11041 ccagtgcgag ccgatatcgc ctagtgccga agttactccg ccctttgcag gatcagagcg
    11101 ccaggagtag acgttcgggt ctgtcatcca gtcctgaaga taaaaaccgt gaataaagct
    11161 caacggtccg gtttcgcctc gtgcaatcat gccgcgcgcc tgttgcacca gcggattgcc
    11221 gcggtagttg aatgtaacta tatgcgccac ttttgcggtc agagctgcat cacggagctt
    11281 tcgtccttct gcggcattca gcgcgagagg cttgtctgag ataacatgct tgcccgcttg
    11341 cagtgcggcc atgatgactg gaagatgaag atgattgggt gtggtgttat ggactacctg
    11401 tatgtctgga tcagcaatca gggccttgta atcgccgtat gcgcggtcaa ctttataggc
    11461 acgtgctttt ttttcggctg attcctgcga tgacccggct atggcaataa cctccacatc
    11521 gcccaggcgg cgcacagcat caagatggtg cgcagcaacg aatccagggc cgaccagccc
    11581 cattccaatc tttttcatgc agacctcaga tgtggattag cgcgttgggc gcgggtgcgg
    11641 agtggtgctg ccttccgtta gccccatggc ccacttaatg gcctcaaagt acatcttgcg
    11701 aatgtcggga tcttcccatg cttcctctgt atgaccaagc gtggagtaga agacgcgtcc
    11761 cttcccatac attttgcacc acgcgaccgc aaaatcatgg tccgtacgat gaatgcgcgg
    11821 attattttcg taattcagct tcgtcggatc gagactcagc agcacattca ccttgtcgcg
    11881 cgaccagtct tttggctggt aaatctcgtc gtacttcaca aattctgttg gaaaatgccg
    11941 gacggcagga aaatcggggg cttcgtttat gatcggcgca ttgaaagtca tccacggatg
    12001 ctggtcgaac catccgccaa tcatctctcc atattcaggc caggtgtaat tggtgtccag
    12061 cgccgcatgg attccgacaa aaccctttcc atcttctttg ataaaagaca tcatgtcgcg
    12121 cttctggtcg gcgtccatgt ccagttcgcc cgttgtactg gcaaagacaa gaacatcaaa
    12181 ataattcaga ttttttgcgt tgcggcccag ttctttcttc gtcagcaatt ctgtgtccgt
    12241 gcgcatctcc gtgtcccaca agccgctctc cttgcccatc tcgtagatgg cgttcatcgc
    12301 agcggaaaca gaatcatgct caaagccctt tgtctggcca atgaccaaca catgcttcag
    12361 atgaatttgt tttgcatggg gagcggccgg taggtacaaa tctttattgg attgctgtgc
    12421 agggctcgta agggaaagtg ccccgaagaa gagaaacgca acaagagagc aggcaaactt
    12481 cctgggatga cgcaaggaaa ctccttctgc agcagaaatg tttcttcggt tatgaagaat
    12541 atcgcttgaa cgatacttct gtgaaggggc cgatataaaa aaagacaatc gcctgtcttc
    12601 tatacatcgc acaaatctac agcttcccgc agcgaggtaa aagggtctct ctgcggaaga
    12661 aattcatgtg ctacgtagcc tgagaagtcc agatcggcaa ttgcacgcat gaccgcggcg
    12721 tactgaattt cctgagtatc gtcaatttca tgcctgcccg gaatgccccc ggtatgaaag
    12781 tgggcaatgg caccaatgtt ctcgcgaatc gtggcaatca ggtcgccttc catgatctgc
    12841 atgtgataaa tgtcataaag caatttcacg cgcggtgaat ttacttcgtg gataacacgc
    12901 gcgccccacg ccgtgtggtc ggccatgtag tcaggatggt tgcgcttgct gttcagcagc
    12961 tccaggcaga tagttacgcc atggtcttca gcaattttct ttacgcgatt cagaccggca
    13021 atggtattgc gcgcgccttc ctcatcagac atcccgcggc ggtttccaga aaatgtgatg
    13081 acgttcggta tgccggcttt tgccgccaca gagatgtttt ctcgaaacgc cctttcgatc
    13141 gcatcgtggt tttccgtgcg gttcaatgca tcggaaatcg tcccgccacc ggcataggcc
    13201 atcgtgcaga tgagtccgta acggcgcggg acctcccagt cattcttttc aagcagatca
    13261 atgcctttga ggccaatctg cgcggcgtag gcacaaagtt gatccagcgg aacatgctga
    13321 taacaccagc ggcaaacgga ctgatggatg cggcctttcc tctgagtggc atgggcaaga
    13381 ggtgcttttg cagcgatagc agcggcaagt ccggatttca gaaaagtgcg gcggtcgggc
    13441 atgataacgc catgttagtt tggcaccccg aaaactcgca aacgcaaatg gatcgttggg
    13501 ctatagcatt tccacagata gtctgaacca tgcctcgaaa ctacagtcgt tacgaccaac
    13561 agggagccgg cggcactgca cagtgcaaat ggagtgtaca gaatctgtga aaatgtctta
    13621 aacactgatc tgtgtgttcg gccaggctgg atagaacacg ccgatctaca agggactaag
    13681 tgttcaatca agttcagaaa catcaggcca aatgatcagg aactccagaa agcagagttc
    13741 ccggacaatg aaaactcgac tctctgaact tatttcctgg tctgtttctt gcgttctgcc
    13801 cagcgttttt tcatggcagc cgatatacgg cggcgggcag cagcgctcat tcgcgtgccg
    13861 cgtacacggc cgggagtgtg gccttttcta tgagggcccg ttgtgttccc ggttagcaac
    13921 gcgcgtgctt gttttagccg gtcaatttct ttgtccagcg cagcaatgat cttctttact
    13981 tccacgggcc cctccgtttg ggtcacagat ataagttccc ttaaattgac taaaaattgc
    14041 ggtgtccaga tcggtttaaa tagatatagc taacaggctt tagtgtaacc atacttatca
    14101 aagattgaca aatgtatcag tgagttgggc gtttaagtat ggtcaggcag cacgacatat
    14161 ttatagggaa actccggtta agtccgcttt tcagagcttt cttagctcat aaagttatag
    14221 acttcatttt aatttttaat gcatgaaact tcctctccat ggttttacag aaaaacatac
    14281 tccctgtagc atcaatggaa ccggccttca gaaataattc caaaatcttc ttgagaacct
    14341 gagcatagat cagagcgaca gaatgtcctg cctccgcgca cggcatctaa aagcagggaa
    14401 ctgcaataaa acattttgcg tatttccagc aggcaatgca gagcgccagc gacatcttcg
    14461 ggcaggtctt tcgcgccatc tgggccaaca agctgcgttc ttttctcacc atgtttggca
    14521 ttgcatgggg cgttggctca atgttgctct tggtgggcgt gggcgaaggg tttcgctcgg
    14581 ggcagaggcg cgagctggca acgctgggca atgatctgat tatgatgtgg ggcgggacca
    14641 tccccgccgt gccgaaccag catacgggaa tgcgtcctta taatttgacg ctctcagatg
    14701 cagaggccat ccgcggcgag gcatctgagg tacgcgctgt gacggcgttc atcaaccgca
    14761 acgacattaa gcagcagagc ctgtatgaaa gcgcaggcgg acaggtcatc ggtgccgagc
    14821 cgaactacag cggtgtgcgc tttcttcctg tcaaagaagg acgttttttg cgtgatgcag
    14881 acatgcgtga ccgccggcgt gtcgttgtgc tcggcgaaaa aagtgcgaag ttgctctttc
    14941 ctgggcgtcc ggcgctggat gagacggtgc tgctgaatgg cgcccgtttt caggtgattg
    15001 gcgttgctgg caagaccgga cgcggcaaca acgacacgga gaaccagaag atttacatcc
    15061 cgctgagcac catgctggag atttttccgg tgaagggtga caacattcct gctgactcgg
    15121 tcacgtccat ccagtaccag ccgcgggtgc gcggagaaaa tgccgcggct aaacagcagg
    15181 tgcatgaaat cattgcccgg cggcacggct ttgaccccaa tgcacaggac gcattcaacg
    15241 aatgggacac cattaagacc gaacagatgg tggggaagat atttacggcg atggatgtct
    15301 ttttgggcgg cgtgggcatt gtgacgctgg cattgggcgc agtaggcatc atcaatatca
    15361 tgcttgtctc agtcac
//
LOCUS       DASRBV010000367         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_283825, whole genome shotgun sequence.
ACCESSION   DASRBV010000367 DASRBV010000000
VERSION     DASRBV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283825"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFA02_13540"
     CDS             complement(<1..905)
                     /locus_tag="VFA02_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU10921.1"
                     /translation="MRKMKLWLALAIAVCLPVFAQEQTGKIHGHIQDPAGVAVTDGIV
                     TLSNDGGKTAKYTFKADANGDYKGDGIAPGTYTVSLRRPDTPPDKVLDQFQNVKITAG
                     EDTAQDFDMTRQDYISKLSPEQRKQLEALKKQNAEAMKENAVIKNLNADLQKAREDNK
                     NKNYAEAEQLMQKDTQAKPDASVLWLELGTAQAGEKKWPDAETSLKKAIELDSQSKKP
                     NPEIQAAANNSLGEVLANQGKIQDAQAAYEAAAKINPAQAGMYYSNEAIVMSRTGQSD
                     ATVAAADKAIAADPNKPIPYYLKGQA"
     gene            complement(995..1687)
                     /gene="queC"
                     /locus_tag="VFA02_13545"
     CDS             complement(995..1687)
                     /gene="queC"
                     /locus_tag="VFA02_13545"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZU10922.1"
                     /translation="MPSTRPKAVVSLSGGMDSCVCAALAARDYDSYAVHFSYGQRTEA
                     KELESARAVAAHLGLKDFLHLKIDLFRRIGGSALTDANIAVPNAPSGGQIGAEVPVTY
                     VPFRNAHFLSAAVSWAEVLGAKKILIGAVEQDSSGYPDCRPAYYEAFQQLIRTGTKEG
                     DIEIETPLIHLRKKEIVALGLELGAPLDLTWSCYSGEETACGVCESCVLRLRAFEEAG
                     TRDRIPYAVHRI"
     gene            1934..2701
                     /locus_tag="VFA02_13550"
     CDS             1934..2701
                     /locus_tag="VFA02_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10923.1"
                     /translation="MAALPLSATAQTSGPGVSSSSDYKAYLSEDIDGASLAAAPTPAP
                     SAKPQYGQTNSYPSYQSRWSHLAFEAGGGFNAPVGHTTNYATYGGNVTIGAGWNFNKW
                     LGTLVEYQFMSDKIPGKTLAAVGAPGGNVHVWSFTIDPILYLPSQGNFGAYITGGGGF
                     YRKVTTFTSPQPVLYCDYFGFCFEGVTNVTIGHFSSNQGGMNIGAGFTWKAFGPDSNG
                     KLFAEVRYVWVDSPSATATQQGTGSFGTLPVTFGVRW"
     gene            complement(2851..3246)
                     /locus_tag="VFA02_13555"
     CDS             complement(2851..3246)
                     /locus_tag="VFA02_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10924.1"
                     /translation="MKAIAQSFFAILAGFVTVVGISGIAAAVLRKFAPSVADENTPAD
                     PFIMAVNVGIGMATSLLGGYVTARFSQGNPLIHALVLALVVLLFSAFSAVQMKNKQPV
                     YYLLVLTVIPPLAVLCGGLLRLRQLGIQW"
BASE COUNT          651 a          948 c          913 g          766 t
ORIGIN      
        1 gcctgtccct tcaggtagta cggaatcggc ttgttcggat ctgcggcgat ggccttgtcc
       61 gcggcggcca cggttgcgtc tgactgccct gtgcggctca tcacgatggc ttcattgctg
      121 tagtacattc cagcctgtgc gggattgatc ttggcagccg cttcgtacgc cgcctgcgca
      181 tcctgtatct tgccctggtt tgccagcacc tcgccgagcg agttattggc tgcggcctgg
      241 atttccgggt tcggcttctt gctctggctg tcaagctcga tggccttctt cagtgaagtc
      301 tcggcgtcgg gccatttctt ctcgcctgcc tgggccgttc ccagctcgag ccacagaacg
      361 gaagcatcgg gctttgcctg ggtgtccttc tgcatcagct gctcggcttc tgcatagttc
      421 ttgttcttgt tgtcttcgcg ggccttctgc aggtcggcat tcaggttctt gatgaccgca
      481 ttttccttca tcgcctcagc attctgcttt ttcagcgctt ccagttgctt gcgctgctcg
      541 ggcgacagtt tggagatgta gtcctgacgg gtcatgtcga agtcctgggc cgtgtcttcg
      601 cccgcagtga tcttcacgtt ctggaactgg tccagcacct tgtcgggagg cgtgtcaggc
      661 cgccgcagag aaaccgtata ggtcccggga gcaatgccat cacccttgta atcgccattg
      721 gcatcggctt tgaaggtgta ttttgccgtc ttgccgccat cgtttgaaag cgtaacgatg
      781 ccgtcggtga cagccacgcc tgcgggatcc tgaatatgac cgtggatctt tccggtctgc
      841 tcctgcgcga acactggcag gcataccgca atcgcgagcg ccagccagag tttcatcttt
      901 ctcatgtttt tctccttaac gcgccagtgt gggcgcgcct gtttaaaatt ctgtctgccg
      961 ggcctgacct atccggaacc aggcccgttt tgtctcaaat acgatgcaca gcgtatggga
     1021 tgcggtcccg cgttccagct tcttcaaaag cgcgcagacg caacacgcag ctctcgcata
     1081 cgccgcaggc agtttcctcg cctgaatagc atgaccacgt taaatccaat ggcgcgccca
     1141 attcaaggcc gagcgcgacg atctccttct tgcggagatg gatgagcgga gtctcgattt
     1201 cgatgtctcc ctcttttgtt ccagtacgta taagttgctg aaaagcctca taataagctg
     1261 gacggcagtc cggatatccg gagctgtcct gctcgacggc gccgatcaag atcttctttg
     1321 cccccaggac ctcggcccag cttactgccg ctgacagaaa atgcgcgtta cggaaaggga
     1381 catacgttac cgggacctcg gcgcctatct ggccgccgga gggcgcattc ggcacagcga
     1441 tatttgcatc ggtcagagcc gagcctccga tacgccggaa gaggtcaatt ttcagatgca
     1501 gaaagtcctt caatcccaga tgcgcggcca cggcacgggc agattccagt tcctttgcct
     1561 cagtcctctg gccatagctg aagtggacgg cataggagtc gtagtcacgg gcagccaaag
     1621 cggcgcagac gcaggaatcc atgcctccag agaggctcac gacggctttg gggcgggtgg
     1681 agggcattca cttttatggt agcgcagcgg tgcatgaaga ggacttccgc cgggccccgg
     1741 gttggcacat ttctatggaa tgacatcgtt taccgagtca aaagcatcaa accagggtta
     1801 accagattgt cagggggccg acccacgaag cccccaatcc aaatacaaca ctgaggggaa
     1861 atctatggtg gtcagttcga gttgttcccg ttcacttttt tgccggattg ccattgcgtt
     1921 ttctgccgta gcaatggctg cgcttccact ttctgccacg gcccagacca gtgggccggg
     1981 agtgtccagt tcatcggact acaaggccta cctgagtgaa gatattgacg gggcctcgct
     2041 tgctgcggca cccactccgg ccccgtcggc caagccgcag tatggacaga caaattcgta
     2101 tccgtcttat caaagccggt ggagccatct ggcttttgaa gcaggcggcg gattcaatgc
     2161 tcctgtaggg cacacgacaa actatgcaac ctatggcgga aacgtcacta tcggggcagg
     2221 atggaatttc aacaagtggc tgggaactct ggtcgaatat caattcatga gtgacaagat
     2281 tccgggcaag accctggcag cggtcggcgc tcccggggga aacgtgcatg tctggtcgtt
     2341 tacgattgac cccattctgt acctgcccag ccaaggaaac tttggcgcgt acattaccgg
     2401 cggcggcggg ttctatcgca aggtgacgac ctttacctca ccacagcctg tgctgtattg
     2461 cgattatttc ggcttttgct ttgaaggcgt caccaacgtc accattggcc acttttccag
     2521 caaccagggc ggcatgaaca tcggcgccgg gttcacctgg aaggccttcg gtccggacag
     2581 taacggcaag ctctttgctg aggtccggta tgtatgggtt gattcgcctt cagccacagc
     2641 cacccagcaa ggtacgggca gctttggaac gcttccagtc acgtttggcg tgcgctggta
     2701 aaacgacagt tgcttttcga ccggcaccct gggtttcgca ggaaaacggg gtgcctcgtc
     2761 cttttgtaac agggtgggag gtgttcgaat caatcccact gcgggaatga tggtgggccg
     2821 gtcttccaca agcctcaagg gtcaattcgt ctaccactga atccccaact gtctcagccg
     2881 caacaagcct ccacacaata ccgccagcgg tggaattacc gtcagaacca agaggtaata
     2941 gaccggctgt ttgttcttca tctgtacggc ggaaaacgcg ctgaacagca gcaccaccag
     3001 cgccaacacg agcgcgtgaa tcagaggatt gccctgagag aaccgcgccg tcacatatcc
     3061 tcccaacagc gatgtggcca taccaatgcc cacgttcacc gccatgatga acgggtcggc
     3121 gggagtattt tcatctgcaa cggagggggc aaatttcctc aggaccgcgg ctgcaatgcc
     3181 ggaaattcca acgacagtga caaatcccgc cagaattgcg aaaaaagatt gcgcgatggc
     3241 cttcatttcc tgtgcatttc cgcttcaacg gcttcggc
//
LOCUS       DASRBV010000368         7277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_30549,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000368 DASRBV010000000
VERSION     DASRBV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30549"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1387
                     /gene="ffh"
                     /locus_tag="VFA02_13560"
     CDS             11..1387
                     /gene="ffh"
                     /locus_tag="VFA02_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZU10925.1"
                     /translation="MFENLSEKLQRAFKNLRGQGTVTEENIGEALREIRVALLEADVN
                     LNVVKDLLEHIRAKALGQEVMTALSPTEQVIKIVRDELVTLLGKDTARFNFASRPPTV
                     ILMAGLQGSGKTTTSGKLAAWLQKGGHRPMLVSVDVYRPAAREQLKVVAKSIKANIYE
                     GDTKGEPAGTALVERLAKEAKREAVISGCDTLIVDTAGRLHIDEDLMGEMESLKKLLS
                     PQEILFVADAMTGQDAVNSADQFHKRLSLTGVILTKMDGDARGGAALSIRHVTGQPVK
                     FIGVGEKPDAFEPFHPDRIVSRILGMGDIMTLIEKAEEKLDRKKSEEFARKALSGDGF
                     SLEDFRDQLRQIRKLGSLQSIMKMLPSVGPLAGMQQMADQVDEKQFVRVEAIINSMTQ
                     KERNNHEIISGSRRKRIAAGSGTTVQEVNQLLRQYAQMRKMFKNVGKGGLLQRRAMGM
                     LSGMKGFR"
     gene            1479..2144
                     /locus_tag="VFA02_13565"
     CDS             1479..2144
                     /locus_tag="VFA02_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HZU10926.1"
                     /translation="MTMIDLDHRLQDQLDEITANTRKLVQPERLAISEQAITDLFSSG
                     IEQQILPVGAKAPEFALPDSSGRTVRSADLLALAPLILNFFRGRWCPYCTTELEAWRD
                     LYPVIRDRGALLIGISPQTVRQSDFTVTQHEIPFPLLHDSHCDVAQLFGLAWKVPPFL
                     QNYYRSILVNIPFINGDESWLLPLPATYVVDQDGTVLFAEAHADFRVRPEPGEVLTHL
                     PKR"
     gene            2325..4745
                     /locus_tag="VFA02_13570"
     CDS             2325..4745
                     /locus_tag="VFA02_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU10927.1"
                     /translation="MQSFVQDFRYAIRQLKKSPGFGLTVIITLALSVGITAAVFSVLY
                     AMVVRPLPYNNPDRIVALETYSSQGYTQPASYPEYLDWRRMNHGFQALAAYNDYSNVN
                     FEGPTGPVALHAVQASDNFFDVFGVNPILGRAFAPGEDQDGRNDVVVLSYEIWQQFFG
                     AQRSVIGQKVKLDGRPYTIIGVMPAGFRYPIGKINAIYTPLHMSKQQREGRGNHWLPT
                     IGRLKSGVTLKQAEADMNSVFADLGRSIPETKGRRVKLLDIGSFILGDTRDSLHLLLY
                     AVLALLAIGCVNVAGLLLARGVKREREMALRSAVGADRLRIIRQMLTEALLFAFCGAL
                     GGVLLAYGLLRVIKMLLIAALSRGSEVGLNVPVLLAALFVSVVVTLLAALAPALRLSG
                     TSPGLALRTGGSAGASRGQHRLRAGFVITQVALALALLVVSGLLMHMLGGLKNTDLGF
                     SPDNILTAEIDLSPGRYDGRDVMADFYNPLFEKIRAIPGVRAVGMIQVLPIQNWGWNS
                     EIHVAGTPIPPPNVNTLAELRIVSPGYFDVFQVPRVSGRLLDPALDTPTSKSVIVVNE
                     AFVKKFIPAGHDPIGMHLDNDEKPIIVGVVKNVRHNITEPPRAEMDYIASQVPKEESL
                     RVLGNMTLVIRTSVPPESIIPALRRAYHDVDPTLPFRTPETMRTIIADRLTFERLENW
                     LFGAFAALAVLLAVVGLYGLISHEVELGTRDIGVRMALGATRARILANIYRRVGWMLG
                     TGVAIGLALTWAVRKYIASVVAMHLEKDAGRILAWTAALVLAGLIAALFPARKASSIE
                     PVEALREE"
     gene            4879..5079
                     /locus_tag="VFA02_13575"
     CDS             4879..5079
                     /locus_tag="VFA02_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZU10928.1"
                     /translation="MEQGTVKWFNDAKGFGFISRQNGDDVFVHYSAIVSSGFKSLQEG
                     QAVQFNVVKGPKGWQAADVQPL"
     gene            complement(5154..5930)
                     /locus_tag="VFA02_13580"
     CDS             complement(5154..5930)
                     /locus_tag="VFA02_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU10929.1"
                     /translation="MVSLPPLDRESVIPLYHQIEQRLLGQIRAGVFKLGEPLPSSQEI
                     ATALGVSPMTVRQAIKSLCELGVIYSEQGKGTFVSGIKIDKNFRQVLSFTEDMKARGS
                     VPSTKVLKFEVRHPSQDVADALRLKPDEEIFHLARLRLADSLPLGIEYCSLPRRLCPD
                     LMERFDPGTSLYEALARSYGIHIAVADEVVEVGFASKQEAQLLKIAKNNPAFLFTRIA
                     YVQSGQPVEHVRSVYRGDRYRVVNRLTRVNRELLEASLAR"
     gene            6216..>7277
                     /locus_tag="VFA02_13585"
     CDS             6216..>7277
                     /locus_tag="VFA02_13585"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HZU10930.1"
                     /translation="MSRKCSGKTLFLSLLSVPLAVLLASGCGSTVNSSPSSGSSGSSG
                     SSSNTGPAFIIATDAPRAGVTSFSVQVQGINAIDSNGNSVPLLSGSPTVDFARYNGLQ
                     TLLDMNDVPAGTYNSIVITLGSASIGYLQTQQGAAPTIQTMPAVLTQSTITENLSSPL
                     VVSQTGPVGIRLDFDLYKSIQVDSNGQITGQVTPVFDVKAIGPSDPGAYIDEFNAGVV
                     SVNASAQSFVIQGPHGHQFTVQVNGQTEWDNNEGLNQLTANSIVQISGVLDRADSTID
                     ADEVAIVSQDGFYAGGLVTYVQPSSGTASSFDLYVRGLLPDSTGLSLGQIAQINLTGN
                     EKFFIYWMHNPLTQFLFNAS"
BASE COUNT         1566 a         2154 c         1989 g         1568 t
ORIGIN      
        1 gcaagccatc atgtttgaaa atctctcaga aaagcttcag cgggccttca aaaaccttcg
       61 cggacagggc accgtcaccg aggaaaacat cggtgaggcc ctgcgggaaa tccgcgtcgc
      121 tctgctcgaa gcagacgtca atctcaacgt agtcaaagac ctgcttgagc acattcgcgc
      181 caaggccctc gggcaggaag tcatgacggc gctctcgcct accgagcagg tcatcaagat
      241 cgtccgcgac gagctggtca cccttctggg caaggacacc gcccgtttta acttcgcttc
      301 gcgtccgcca acagtcattc tgatggcggg tcttcagggt tcaggtaaga cgacaacctc
      361 gggcaaactc gcagcatggc tccagaaggg cggccatcgc cccatgctcg tctctgtgga
      421 cgtctaccgc cccgcagccc gcgagcagtt gaaggtagtt gcgaaatcca tcaaggccaa
      481 catctacgaa ggcgatacaa agggcgagcc agcaggcacg gccctggtcg agcggctggc
      541 gaaggaagcg aagcgcgaag ccgtcatctc cggttgcgac acccttatcg tggacaccgc
      601 cggacgcctc cacattgacg aagacctgat gggcgaaatg gaatcgctca agaagctgct
      661 ctcgccgcag gaaattctct tcgtcgccga tgccatgacg gggcaggacg ccgtcaactc
      721 cgccgaccag ttccataaac ggctcagcct gacaggcgtc atcctcacca agatggacgg
      781 agatgctcgc ggcggtgctg cgctctcgat ccgtcacgtg actggacaac cggtaaagtt
      841 catcggcgtg ggtgaaaagc cggatgcctt cgagccgttc catccggacc gcatcgtcag
      901 ccgcattttg ggaatgggcg acatcatgac gctcatcgaa aaggccgagg aaaaactcga
      961 ccgcaaaaag tcagaagagt tcgcgcgcaa ggcgctctcc ggcgacggct tttcattgga
     1021 agatttccgc gatcagctta ggcagattcg caagctcggc tcattgcaga gcatcatgaa
     1081 aatgctgccc tccgttgggc cgctcgcagg catgcagcag atggccgacc aggtagacga
     1141 aaagcagttc gttcgcgtcg aggccatcat caattccatg acgcagaagg agcgcaacaa
     1201 ccacgaaatc atctccggca gccggcgcaa gcgcatcgcc gccggatcgg ggaccactgt
     1261 gcaggaagtc aaccagcttc tgcgccaata cgcgcagatg cgtaaaatgt tcaaaaatgt
     1321 tggcaagggt ggactgctcc agcgccgggc catgggtatg ttgagcggca tgaaaggctt
     1381 cagataaacc tggtcacccc tgccgactca acccggcaag cgaagaccag accggccgct
     1441 ttagctgact gcgccgacag gcgcgctgac tcgctaagat gactatgatt gacctcgacc
     1501 atcgcctgca ggaccagctc gacgaaatta ccgccaacac ccgcaaactg gtgcagccgg
     1561 agcggctggc catcagcgag caggccatca ccgacctctt ttccagcggg attgagcagc
     1621 agattcttcc tgttggcgca aaggcccccg aatttgcctt gcctgactcc tccggacgca
     1681 ccgtgcgctc cgctgacctg ctagcacttg ctccactcat cctcaacttc tttcgcgggc
     1741 gctggtgtcc ctactgcaca acggaactcg aagcctggcg cgacctctat cctgtcatcc
     1801 gcgaccgtgg agcgctgctg atcggcatct cgccacagac cgtccggcag agcgacttta
     1861 cggttaccca gcacgagata ccctttccgc tgctgcatga ttcccactgc gatgtggccc
     1921 agcttttcgg cctcgcctgg aaggtcccgc cctttctcca gaactattac cgcagcattc
     1981 ttgtaaatat tcccttcatc aatggtgacg aaagttggct tcttcccctg cctgcaactt
     2041 acgtggttga ccaggacggc accgtcctct tcgccgaggc ccacgccgat tttcgcgtcc
     2101 gccccgagcc aggtgaagta ttaacacatt tgccgaagcg ttgattgttt attacccgct
     2161 acaaaaacag cacgactgtc cgtatatgga catcccacgt ccggaaatgg acacaacctc
     2221 tatgttggtt cgatcgtaaa tcctctattt cattgatttc aaagagctcc tcctgcctga
     2281 acaaatggca atttacgtgc gtgtacagga agagaggctt cgccatgcaa tcattcgtgc
     2341 aggatttccg ctacgccata cgacagttga aaaaatcgcc tggattcgga ctgaccgtta
     2401 tcatcacact ggccctcagt gtgggcatta ccgccgccgt cttcagcgtg ctgtatgcaa
     2461 tggtggtgcg tccgttgccg tacaacaatc cggacagaat cgttgcgctt gaaacctact
     2521 cttctcaggg atatacccaa ccggcctctt atccggaata ccttgactgg cggcgaatga
     2581 accacggctt ccaggcgctg gcggcctata acgactactc caatgtaaat tttgaaggtc
     2641 caacggggcc ggtcgcgctc catgccgtgc aggcttccga caacttcttt gatgtttttg
     2701 gtgtgaaccc gattctgggc cgcgcctttg ctcccggcga agaccaggac ggcaggaatg
     2761 atgtcgtagt cctcagctac gaaatctggc agcagttctt cggagcacag cgcagtgtga
     2821 ttggccaaaa ggtgaagctt gatggccggc cttataccat cattggcgtc atgcccgctg
     2881 gatttcgcta tcccattggc aagatcaatg cgatttatac gccgttacat atgtcgaaac
     2941 agcagcggga aggccgcgga aaccactggc tgccgaccat tggcagactg aaatccggcg
     3001 tcacgctgaa acaggccgaa gctgacatga acagcgtctt tgcagacctg ggacgctcta
     3061 ttcctgaaac caaaggacgg cgggtcaaac tgctcgatat tggaagtttc atccttggcg
     3121 atactcgtga ctcattgcat ctgctgcttt atgccgtact tgcgctgctt gcgattggct
     3181 gcgtcaacgt ggccggactc ctgctggccc gcggcgtcaa gcgcgagcgt gagatggcgc
     3241 tgcgctcggc tgtaggcgcg gacaggctac gcatcattcg gcagatgctg accgaagcat
     3301 tgctgtttgc tttttgcggg gcgctaggtg gcgttctgct ggcctatggt ttgctgcgcg
     3361 tcatcaagat gcttttgatt gcggcacttt cgcgcggttc ggaagtcggg ctgaatgtgc
     3421 ccgtactgct ggctgcttta tttgtatctg tggtagttac attgctggcg gcgcttgcac
     3481 ctgcgctgcg cctctccgga acatcaccgg gcctggcgct gcgtaccgga ggaagcgccg
     3541 gtgcatcacg cgggcagcac cgcctgcgtg caggctttgt gattacacag gtggccctcg
     3601 ctcttgcgct gctggtggtc tccggtttgc tgatgcacat gctgggcggc cttaaaaaca
     3661 cagacctcgg cttctctccg gacaacattc ttaccgcaga aattgatctt tcgcccgggc
     3721 gctatgatgg ccgcgatgtg atggcggact tctacaatcc actgtttgag aagatacgcg
     3781 ccatccccgg agtgcgggca gtaggcatga ttcaggtgct gcccatccag aactggggat
     3841 ggaactcaga aatccatgtt gccgggacac ctatacctcc gcccaatgtg aacacgctgg
     3901 cggagctgcg catcgtgtct cctggctatt tcgatgtctt tcaggtccct cgggtgagtg
     3961 ggcgtttgct cgatccggcc ctggacacac caacatcaaa gtctgtcatc gtcgtcaatg
     4021 aagcttttgt aaaaaagttc attcctgcag ggcacgaccc cattggcatg caccttgata
     4081 atgatgagaa gccgatcatt gtcggggtcg taaaaaatgt ccggcacaac atcacggaac
     4141 cgccacgcgc ggagatggat tatatcgcct cccaggtgcc taaggaagaa agcctgcgcg
     4201 tgttagggaa catgacactc gtcatccgca cgagcgttcc gccggagagc atcatccctg
     4261 ccctgcgccg cgcttatcac gatgtggatc cgacgctccc tttccgcaca cctgagacga
     4321 tgcgcacgat catcgccgac aggcttacct ttgaacggtt ggagaattgg ctctttggcg
     4381 cctttgccgc gctggctgtg cttctggcgg ttgtgggcct ctatgggctt atcagccatg
     4441 aggtcgagct tggaacgaga gacattggcg tgcgcatggc cctgggtgcg acacgcgcgc
     4501 ggattctggc gaatatctac cgccgcgtag gctggatgct gggcaccggc gttgccattg
     4561 gccttgcgct gacctgggcg gtccgcaaat acatcgcttc cgtagttgcc atgcatctgg
     4621 aaaaagatgc gggccggatt ctggcgtgga cagcagcact ggtccttgcc ggactgattg
     4681 cagcgctgtt ccctgcccgg aaagcgtctt ccatcgaacc tgtcgaggcg ctgcgggaag
     4741 aataaaatcc agaatctggt agactgtaaa ggcgttagaa ccggaccagg caaaagcagc
     4801 acctcgccta ccagcaagat tctgccagaa gttggctccg ttggctccag cgcaatccca
     4861 attaggaaat agcatcacat ggaacaaggt accgtaaagt ggtttaacga cgcgaagggt
     4921 ttcggcttca tttcgcggca gaatggcgat gatgtcttcg tgcattattc cgccattgtc
     4981 tcgagtggct tcaagagcct tcaggaaggc caggccgtcc agttcaacgt cgttaagggg
     5041 cccaagggct ggcaggcagc ggacgttcag ccgctctaag acgcaacaga aataaatata
     5101 agatctaata cctgatcaat gatttgaggc gggctgcggc ccgcctttgg ttttcaccgc
     5161 gccagagagg cctccagtaa ctcacggttg acgcgcgtca gccggttcac cacgcggtaa
     5221 cggtctcccc tgtaaacaga gcgcacatgc tccactggct ggccgctttg tacatacgcg
     5281 atccgcgtaa acagaaatgc cggattgttc tttgcaatct tgagcagttg cgcttcctgc
     5341 ttgctggcga aacccacctc caccacctcg tcggctactg caatgtggat tccatagctc
     5401 cgcgccagcg cttcatacag cgaagtccca ggatcgaacc gctccatcag gtctgggcac
     5461 aagcggcgcg gcagcgagca gtactcgatg cccagcggaa gcgaatcggc cagacgcagg
     5521 cgtgccaggt gaaagatctc ttcgtccggc ttcagacgca gagcatccgc cacgtcctga
     5581 gaaggatgcc gcacctcaaa cttcaacacc tttgtgctgg ggaccgagcc gcgcgccttc
     5641 atgtcctcag tgaaggacag tacctgcctg aagttcttgt ctatcttgat accggaaacg
     5701 aaagtaccct ttccctgctc gctgtaaatt acgcccagtt cgcataatga cttgatggct
     5761 tgccggaccg tcatcgggct gacgcccagc gctgtcgcaa tctcctgcga agagggcaat
     5821 ggctcaccca gcttgaatac gccagcccgt atctggccca gcagacgctg ctcaatctgg
     5881 tggtaaagcg gaatcacact ttcgcggtcg agtggtggta gggaaaccat tgttttgcgt
     5941 gtttctggct gattgactat ataacacaaa gaccagacag tttcccaaag cgaaatttcc
     6001 ggcgaaactt ttcagaacac cctgtgttac aaatggcagc gcccgttctg gcgcaatacc
     6061 aatttccagc actctcttgc tgggcaaaca tctgtaggtc tttttccttt tctccgagag
     6121 ggcacgtaac aaaaaagcgc tttggcgcgc agcgggagat gtgtgcctgc ggatgtttgg
     6181 tcagcaaaag ggctgtacaa gaagggtgac ccacgatgtc tcgcaaatgt tcaggcaaga
     6241 cgctatttct ctctctctta tcggtcccgc ttgctgttct gctggcaagc ggctgcggct
     6301 ccacagtcaa ctcctcaccg tcttccggtt ccagcggaag ctccgggtcc tcgtcgaata
     6361 ccggtccggc attcatcatt gcaaccgatg ctccgcgcgc tggcgtgacc tcgttctcgg
     6421 tgcaggtgca gggcatcaat gccattgact caaacgggaa cagcgttcca ctcctcagcg
     6481 gctctccaac ggtggatttt gcccgctaca acggcctgca aaccttgctc gatatgaatg
     6541 atgttccggc agggacctac aacagcattg tgattacgct gggcagcgcc agcatcggct
     6601 atctgcagac acagcagggc gcagccccaa cgatccagac catgcctgcg gtccttacgc
     6661 agtccaccat tacggaaaac ctgagctcgc cgctcgtggt ttcgcagacg ggcccggtcg
     6721 gcatccgcct ggactttgat ctgtacaagt ccatccaggt ggacagcaac ggccaaatca
     6781 caggccaggt cacgcctgtc ttcgatgtga aagccatagg cccgagtgat cccggcgcgt
     6841 acattgatga gttcaatgcc ggagtcgtca gcgtcaatgc ctctgcgcaa tccttcgtta
     6901 ttcagggccc acacggacat cagttcactg tccaggtgaa tggacagacc gagtgggaca
     6961 acaacgaggg cctcaaccag cttacggcca acagcattgt gcagatatcc ggcgtgctcg
     7021 accgggccga ttcaacaatt gatgcagatg aagtcgccat cgtctcacag gacggtttct
     7081 acgccggtgg tctggtcacg tatgtgcagc cctcgagcgg gacggcatcg agcttcgacc
     7141 tctatgttcg cggactgctg cccgattcca ccggactctc actcggccag attgcgcaaa
     7201 tcaatctgac aggcaatgag aagttcttca tctactggat gcacaaccct ctgacccaat
     7261 tcctgttcaa tgcaagc
//
LOCUS       DASRBV010000369         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_136809, whole genome shotgun sequence.
ACCESSION   DASRBV010000369 DASRBV010000000
VERSION     DASRBV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136809"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VFA02_13590"
     CDS             <1..1308
                     /locus_tag="VFA02_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZU10931.1"
                     /translation="DLDVFYPTQLLVTGFDILFFWVARMIMLGCHFMLDVPMPDGSKR
                     GLKDAVPFREVYIHALVRDAERQKMSKTKGNVIDPIEIVSKYGTDAVRFTLAAMASPG
                     TDIAFSEARTEGYRAFANKIWNAARFIFMNVDKAAEAGIAVDPQLLADAPASIEARWI
                     TSRLNRTAAEVNQALGEYRFHEAANLVYQFFWGDFCDWYLEIVKLRLDFSQSASRDVS
                     KSALMTLLSTFEAALRLLSPFMPFLTEELWHAVYDGKPPAKSIALSRYPQPHSDAQNF
                     TAETDMAVLQELIVTVRALRKDLDVPEREPVAIEISASEHVWQLAASNRDIVEKLARV
                     SAIEFSAAWRLGPANTRTTANFTVGTPYEKQIDVVAERERLHKKLEQYEKILANAKRQ
                     LDNEAFLAKAPEKVVSGLKKQAEEAAMLRQETLDALEKLEQFA"
     gene            1308..2192
                     /gene="nadC"
                     /locus_tag="VFA02_13595"
     CDS             1308..2192
                     /gene="nadC"
                     /locus_tag="VFA02_13595"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZU10932.1"
                     /translation="MDWKSRRIESVLEQALIEDKATHDVTTAVTIDPSLRASASIIAK
                     QNCVVSGLGCISRFLEIFARLDGRNHGRYEVVHHPEIFDGVRVTKGHALAVIRHNASV
                     ILSCERVILNLMQRMSGIATLTRQYVDAIAGTNAHILDTRKTIPGLRILDKYAVRCGG
                     GQNHRLDLSDGILIKNNHISLGGGLEKVLKRAHELRQPGQTIDVEVRTFDELKVALDN
                     GAESLLLDNMTPEEVKKAVGIVRERGLNIPIEASGGIHLENVRKYALAGVDYISVGAL
                     THSAVAVDLSMKITAEIY"
     gene            complement(2280..2720)
                     /locus_tag="VFA02_13600"
     CDS             complement(2280..2720)
                     /locus_tag="VFA02_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZU10933.1"
                     /translation="MLSFVVYMTERTTPIRVLVAKPGLDGHDRGAKVIARALRDAGME
                     VIYTGLRQTPEMIVNAAIQEDVDCVGLSILSGAHNAIVPRITSLLKERAADDILVVLG
                     GTIPEQDIPFLTSQGVSAIFGPGTSLEAVVSFIRQNVKPRGVPA"
BASE COUNT          608 a          765 c          769 g          584 t
ORIGIN      
        1 gacctcgacg tcttctatcc gacgcagttg ctggtcaccg ggttcgacat tctcttcttc
       61 tgggtggcgc gcatgatcat gctcggctgc catttcatgc ttgacgtgcc gatgcccgac
      121 ggctccaaac gcgggctgaa agatgccgtg cccttccgcg aggtctacat ccacgcgctg
      181 gtgcgcgacg ccgagcgcca gaagatgtcc aagaccaagg gcaatgtgat tgatcccatc
      241 gagatcgtca gcaaatacgg gaccgatgcc gtgcgcttca cgctggccgc gatggcctct
      301 cctgggacgg acattgcctt cagcgaggcc cgcacagaag gctatcgcgc ctttgccaat
      361 aaaatctgga atgccgcgcg cttcatcttc atgaatgtag acaaagcagc cgaggccggc
      421 attgccgttg acccgcagct tctcgccgat gcgcctgctt ccatcgaggc ccgctggatc
      481 acctcgcggc tgaatcgcac cgcggcagaa gtaaaccagg cattgggcga ataccgcttc
      541 catgaggcgg caaatctcgt ctatcagttc ttctggggag acttctgcga ctggtatctg
      601 gaaatcgtaa agctgcggct tgatttcagc cagtcagcaa gtcgggatgt cagcaaatca
      661 gccttgatga ccctcctaag tacatttgaa gccgcgctgc gtctgctctc tcccttcatg
      721 ccatttctta cggaagagct ttggcacgct gtttacgatg gaaaacctcc ggcaaagtcc
      781 atagctttgt cgcgctatcc gcagccccat tcagacgcgc agaattttac ggcagagact
      841 gacatggctg tcttacagga gcttatcgtg accgtccgcg ccttgcgcaa agacctggat
      901 gtgccggagc gcgagccggt ggcgattgaa atttcggcgt ctgaacatgt ttggcaactc
      961 gccgcttcca atcgcgacat tgtcgagaag ctggcgcgtg tttctgcgat tgaattttca
     1021 gcagcctgga ggctcggccc cgccaatacg cggaccactg cgaatttcac cgtgggtacg
     1081 ccttatgaga agcagattga tgttgtggct gaacgtgaac gcctgcacaa aaaactcgag
     1141 cagtatgaaa aaattctggc caatgcaaag cggcagttgg ataatgaagc gtttctggcc
     1201 aaggcaccgg agaaagttgt gtctggcctg aaaaagcagg cagaggaagc ggccatgctg
     1261 cggcaggaaa cactggatgc actcgaaaaa ctggagcagt ttgcataatg gattggaaga
     1321 gcaggcggat tgaaagtgtt cttgagcagg cgttgattga agacaaggca acccacgatg
     1381 tgaccacggc agtcacgatt gatccttcgc ttcgcgcttc ggcctcgatc atcgccaagc
     1441 agaactgtgt tgtttccgga ctgggctgca tctcgcgctt tctggaaatc tttgcgcggc
     1501 tcgacggccg taatcatggc cgctatgagg tcgtccatca tccggagata ttcgacggcg
     1561 tgcgcgtcac caaaggacac gcgcttgcgg tcatccgtca taatgccagc gtgatccttt
     1621 cctgcgagcg cgtcattttg aacctgatgc agcgcatgag cggcattgct actctcacgc
     1681 gccagtacgt ggacgccatc gccgggacca acgcccacat cttagacaca cgcaagacca
     1741 tccccggctt gcgcatcctg gacaaatacg ccgttcgctg cggaggaggc cagaaccatc
     1801 gtctcgatct ttccgatggc attctcatca agaacaatca catctcactc ggcggaggct
     1861 tagagaaggt cctgaagcgc gcgcatgagc tgcgtcagcc cggccagacc attgatgttg
     1921 aggtgcgcac cttcgatgaa ctgaaagtcg cgctcgacaa cggcgcggag tcgttactgc
     1981 tcgacaacat gactcctgag gaagtaaaga aggccgtggg catcgtgcgc gaacgcggcc
     2041 ttaacattcc gattgaagcc tcaggcggca tccatcttga gaatgtccgc aaatatgcgc
     2101 ttgctggcgt ggattacatt tccgtgggtg cgctcacgca ctccgcagtt gccgtagacc
     2161 tgagcatgaa gattacggct gagatttatt aggagatcgc tgaaggtctt tcctctcgtt
     2221 ggtcagtatg accacctcgc cagattttcg cgttgtaaaa agcaatgaga agaaccaaat
     2281 caggcgggga cgccacgcgg cttcacattc tgccggataa agctgacgac agcttccaga
     2341 gaggttccag ggccgaagat ggcgctgaca ccctgagacg tcagaaaagg gatgtcttgt
     2401 tccgggatgg tgccaccgag gacgacgagg atgtcatcgg cggcgcgctc cttcagcagg
     2461 gacgtaatac gcggaacaat ggcattgtgc gctcctgaaa gaatgctcag gccgacgcaa
     2521 tccacatctt cctgtatggc ggcgttgacg atcatctccg gcgtctgacg cagaccggtg
     2581 tagatgactt ccattccggc atcacgcagc gcccgggcaa tcactttcgc gccccggtcg
     2641 tggccatcca gtcctggctt ggctacaagg acgcgaattg gagtagtgcg ctcggtcata
     2701 taaacgacaa aggataacac aggtct
//
LOCUS       DASRBV010000370         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_705847, whole genome shotgun sequence.
ACCESSION   DASRBV010000370 DASRBV010000000
VERSION     DASRBV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705847"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..457
                     /gene="rpsL"
                     /locus_tag="VFA02_13605"
     CDS             56..457
                     /gene="rpsL"
                     /locus_tag="VFA02_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZU10934.1"
                     /translation="MPTFSQLVRKGRTAPKYKTASPALQACPQRRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DSVGVAKRTQSRSKYGAKRAKGGAAAGGGKK"
     gene            524..994
                     /gene="rpsG"
                     /locus_tag="VFA02_13610"
     CDS             524..994
                     /gene="rpsG"
                     /locus_tag="VFA02_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZU10935.1"
                     /translation="MPRKGHIAKREVAGDPVYSSTLVTKFINSMMWDGKKSTAQAIFY
                     TAMKNLEQKGGDEALKLFKKAVENCKPVLEVKTRRVGGANYQVPVEVNPDRRTSLAIR
                     WLITYGRQRGEKGMIDKLTNELLDAANGRGGAIKKKEDVHRMAEANKAFAHYRW"
     gene            1114..3195
                     /gene="fusA"
                     /locus_tag="VFA02_13615"
     CDS             1114..3195
                     /gene="fusA"
                     /locus_tag="VFA02_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZU10936.1"
                     /translation="MARQVPLTRCRNIGIMAHIDAGKTTTTERILFYTGITHRIGEVH
                     EGTATMDYMEQEQERGITITSAATTCFWNNYRINIIDTPGHVDFTAEVERSLRVLDGA
                     VALFDSVSGVQPQTETVWRQGDKYKVPRLCFVNKMDKAGADFEHVIETIRKRLGARPV
                     AIQIPIGAESNFKGVVDLIEMRAILWHDETMGAKYSVEPVPAELQKKAEAFRMQLIET
                     VAENDDDMLHKFLEGETPTPEELKKALRKATIDMKVFPVLCGSAFKNKGVQTLLDAVV
                     DYLPSPLDIPAVQGVDPHDTSVVLTRETSDDAPFSALAFKLINDPFGKLSFIRVYSGS
                     LKTGDTVLNPRTGKTERVGRLVKMHANKREDITEILAGDICACVGLKEIKTGDTLCVP
                     SAPIALGAIDFPEPVISVAIEPKTKGDQEKMALALAKLADEDPTFKVRTDQESGQTII
                     SGMGELHLEILVDRLKREHKVEANVGEPKVAFRETIRKSAEAEGKYIRQTGGSGNYGH
                     VKIRIEPNEPGKGYEFIDEIKGGVVPREYIKPTEQGIREASLSGVLAGYEMVDFKVTL
                     FDGSYHEVDSNEMAFKIAGSMAFKEAAKKANPVLLEPVMAVEVTVPEEHMGTIIGDIN
                     SRRGRIEGMEHVGGSQVIKAMVPLKEMFGYVNDIRSSTQGRASYTMQFARYEEAPRMI
                     SEEIIGRNQGK"
     gene            3270..>4187
                     /gene="tuf"
                     /locus_tag="VFA02_13620"
     CDS             3270..>4187
                     /gene="tuf"
                     /locus_tag="VFA02_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZU10937.1"
                     /translation="MAKEKFDRTKPHVNVGTIGHIDHGKTTLTAAITKVLSKHNPNIK
                     FRSFDTIDNAPEERERGITIATAHVEYETPNRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTKEHVLLARQVGVPYIVVFLNKCDAVEDPELIDLVEMEVRELLN
                     KYNFPGDDVPVVRGSALGALNGEAQWEAKIDELMEAVDKNVPLPQRAVDQPFLMPIED
                     IFSISGRGTVVTGRIERGKVKVGEEVEIVGFRETRKTVVTGVEMFKKQLDEGLAGDNA
                     GLLLRGTAKDEVERGMVLAKPGSITPHTKF"
BASE COUNT          939 a         1037 c         1395 g          816 t
ORIGIN      
        1 gcgtcgtgtt ttggcgtgtc cgcaggtaag tttttgagtt ggtaaggagt ctggattgcc
       61 tacattcagt cagctcgtga ggaaggggcg caccgccccg aagtataaga cggcctcgcc
      121 cgcattgcag gcctgtccgc aacggcgcgg cgtctgcacg cgtgtttaca cgcagacgcc
      181 caagaagccg aactcggcgc tgcgcaaggt ggcacgtgtc cgcctgacga acggcattga
      241 agttacgtcg tacatcccag gtgagggcca taacctgcag gagcactcaa ttgtgctcat
      301 ccgtggcggt cgtgtgaagg acctgccggg tgtgcgttat cacattgttc gcggcacgct
      361 tgactcggtc ggcgtggcca agcgcacgca gtcgcgctcg aagtatggag ccaagcgcgc
      421 caaaggtggc gctgcggctg gcggtggcaa gaagtaatca gatgtcatcc cgggcgtagt
      481 gaagggttct gtttttttga aagcttgaag gtcaaaaggt tttatgccga gaaaaggtca
      541 cattgcgaag cgtgaggttg caggcgatcc ggtctacagc tccacgctgg ttacgaaatt
      601 catcaactcg atgatgtggg acggcaagaa gtccacagcg caggcgattt tttataccgc
      661 catgaagaac cttgagcaga agggcggcga tgaggccctg aagctgttca agaaggcggt
      721 ggagaactgc aagcctgttc tggaagtgaa gacccgccgc gttggcggtg cgaactatca
      781 ggtgccggtg gaagtgaatc cggaccgccg cacttcgctg gcgattcgct ggctgattac
      841 ctatgggcgt cagcgcggtg aaaagggcat gattgacaag ctgaccaacg aattgctcga
      901 cgccgcgaat ggacgtggtg gcgcgatcaa gaagaaagaa gacgtgcacc gcatggccga
      961 ggccaacaag gcctttgccc actatcgttg gtgaggactg gccgtccggg cggacgcgcc
     1021 ttcggcgcat gaggaatgtt gggagcggaa ggttgctccc gaaagaaatt cgaaccttgg
     1081 gccaaaggcc tgagagaact agagagaatt actgtggctc gccaagtacc gttaactcgt
     1141 tgccggaaca tcggaatcat ggcgcatatt gacgccggaa agacgacgac gaccgagcgc
     1201 attctgtttt atacgggcat tacgcaccgc atcggcgaag tgcatgaagg cacggcgacg
     1261 atggactata tggagcagga gcaggagcgt ggtatcacga ttacctccgc tgcgaccacc
     1321 tgcttctgga acaactaccg gatcaacatt attgatacgc ccgggcacgt ggactttact
     1381 gccgaagtcg agcgctcgct acgcgtcctt gacggagcgg tggccctgtt tgactctgtt
     1441 tccggcgttc agccccagac ggagaccgtg tggcgccagg gcgacaagta caaagtcccc
     1501 cggctttgct ttgtcaacaa gatggacaag gcgggtgccg attttgagca cgtcattgag
     1561 accatccgca agcgtcttgg agcgcgtccg gttgctattc agattccgat tggcgccgag
     1621 tcgaatttca aaggtgttgt tgacctgatt gagatgcgcg ccattctctg gcatgacgag
     1681 accatgggcg cgaagtactc ggtcgagcca gtccctgccg agcttcagaa aaaagcggaa
     1741 gccttccgta tgcagctgat tgagacagtc gccgagaacg atgacgacat gctgcacaag
     1801 tttctggaag gcgagacccc gactcctgag gagctgaaga aggcgctgcg caaggcgacg
     1861 attgatatga aggtcttccc ggtcctgtgc ggatcggcct tcaagaacaa gggtgtacag
     1921 acgctgctcg atgcagtggt ggattatctg cccagcccgc tggacatccc ggcggtgcag
     1981 ggtgtggatc cgcacgatac ttctgtcgtc ctgacgcgcg agacgagcga tgatgctccg
     2041 ttctctgcgt tggctttcaa gctgattaac gatcctttcg gcaagctgag ctttatccgg
     2101 gtctactcgg gttcattgaa gacgggcgac accgtgctca acccgcgcac gggcaaaacg
     2161 gagcgcgtgg gccgtctggt gaagatgcat gccaacaagc gtgaggacat cactgagatt
     2221 ctggctggcg acatctgcgc ctgcgtgggt ctgaaggaaa tcaagacggg cgatacgctt
     2281 tgtgtgcctt ctgccccgat tgcgctgggt gcgattgatt ttcctgagcc ggtcatttcg
     2341 gtggcaattg agccgaagac aaaaggcgac caggagaaga tggcgctggc cctggcgaag
     2401 ctggccgatg aagatccgac attcaaggtc cgtaccgacc aggaatcggg ccagacgatc
     2461 atcagcggca tgggtgagtt gcatcttgaa attctggtgg accgcctgaa gcgcgagcat
     2521 aaggtggaag ccaacgtcgg tgagccgaag gtggccttcc gcgaaacgat ccgcaaatct
     2581 gccgaagcag aaggcaagta catccgtcag acgggtggtt cgggtaacta tgggcacgtc
     2641 aagatccgta ttgagccgaa tgagccgggc aagggctacg agttcattga tgagatcaag
     2701 ggcggcgtgg ttccccgtga gtacatcaag ccgaccgagc agggtattcg cgaagcatca
     2761 ttgagtggcg tactggccgg ctacgagatg gtggacttca aggtgacact gttcgacggg
     2821 agctatcacg aagtggactc gaacgaaatg gccttcaaga tcgccggttc gatggccttc
     2881 aaagaggcag cgaagaaggc gaacccggtg ctgctggagc cggtcatggc ggtcgaagtc
     2941 accgtgccgg aagagcacat gggcacgatc atcggcgaca tcaactcgcg ccgcggacgt
     3001 attgaaggca tggagcatgt gggcggttcg caggtgatca aggcgatggt gccgctgaag
     3061 gagatgttcg gatatgtgaa cgatatccgc tcttcaacgc agggccgcgc ttcgtacaca
     3121 atgcagtttg cccgctatga agaggccccg cgcatgattt ctgaggagat catcgggcgt
     3181 aaccagggta aataggtttt ttgagaatca tgccgggctt cgatgcgagg cccggtgaat
     3241 tttgagtcac acgagagagg aactgaggaa tggcgaagga gaaatttgac cggacgaagc
     3301 cgcacgtaaa tgtggggacg atcgggcaca ttgatcatgg gaagacgacg ttgacggcgg
     3361 cgatcacgaa ggtgttgtcg aagcacaacc cgaacatcaa gttccgctcg tttgacacga
     3421 tagacaatgc gccggaagag cgggagcgcg gaattacgat agcgacggcg cacgtggagt
     3481 atgagacgcc gaaccgtcac tatgcgcatg tggactgccc cgggcacgcg gactacatca
     3541 agaacatgat cacgggcgcg gcgcagatgg acggtgcgat tctggtggtg gcggcgaccg
     3601 atgggccgat gccgcagacc aaggagcacg tgctgttggc ccgtcaggtg ggcgtgccgt
     3661 acatcgtggt gtttctgaat aagtgcgatg cggtggaaga ccctgagctg atcgacctgg
     3721 tcgagatgga agtgcgggag cttttgaaca agtacaactt ccccggggac gatgtgccgg
     3781 tggtgcgcgg ctcggctttg ggcgcgctga acggggaagc gcagtgggaa gcgaagatag
     3841 atgagctgat ggaggcggtg gacaagaatg ttcctctgcc gcagcgggcg gtggaccagc
     3901 cgttcctgat gccgatagaa gacatcttct cgatttcagg ccgtgggact gtggtgacgg
     3961 gccgaattga gcgtggcaag gtgaaggtgg gcgaggaagt ggagattgtg ggcttccgcg
     4021 agaccagaaa gactgtagtc acgggcgtag agatgttcaa gaagcagctg gacgagggcc
     4081 tggctggaga caatgccggg ttgctgctgc gtggcacggc gaaggacgag gtggagcgcg
     4141 ggatggtgct ggccaagccc ggttcgatta cgccgcacac gaagttc
//
LOCUS       DASRBV010000371        10283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_182115, whole genome shotgun sequence.
ACCESSION   DASRBV010000371 DASRBV010000000
VERSION     DASRBV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182115"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..640)
                     /locus_tag="VFA02_13625"
     CDS             complement(38..640)
                     /locus_tag="VFA02_13625"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZU10938.1"
                     /translation="MENHLNLTVAITGASGSVFGQQLLQALEADARVDRVHLVVSESA
                     LRVIAEELGLSGRNALVEKLPGQSSSKILQHAESDIGAPIASGSYPSNGMIILPCSMG
                     TLAGIAHGLAQNLVERAADVCLKERRPLVLCVRETPFNKIHLRNMSLASDAGATIFPV
                     IPTFYNHPVDSTAMAKQFVARVLAHIGLPQADAYVWKGEE"
     gene            complement(742..1341)
                     /locus_tag="VFA02_13630"
     CDS             complement(742..1341)
                     /locus_tag="VFA02_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZU10939.1"
                     /translation="MSFTPTTIAALIIAASFAAGLNVYATVLTLGLLAHVHWIALPAG
                     LDILNNWWVIGVSGAMFVIEFFADKVPGFDMIWNALHTFVRVPVAALLAYRASEQLSP
                     EMQLLAAVAGAGIAIVSHGSKTAVRAAVTPTPEPVSNIALSTSEDVVAIGVTWLATRH
                     PFVAATIAVIFLILAVITVRWLARFIRRMWNRPKPANAA"
     gene            complement(1345..1836)
                     /locus_tag="VFA02_13635"
     CDS             complement(1345..1836)
                     /locus_tag="VFA02_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10940.1"
                     /translation="MRKERHVVRFLMFAAFSLSAALAQKDVSVPQSCTPQVNQKLGDL
                     LHANQTQAVDNVMVCGITVSSSRTQRGGPHGNHEILPLRVTFPDGSTRLVEVVTNDDL
                     DGVVTAPVNAQVYAYGQAFFPSRGHFVAGIHDVHCSTHRGADNGWVVVNGQKYPASCS
                     ALR"
     gene            complement(1867..2874)
                     /locus_tag="VFA02_13640"
     CDS             complement(1867..2874)
                     /locus_tag="VFA02_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZU10941.1"
                     /translation="MRAAVLRHTAPVEQKPLQVEEVPELQPAAGEVLLKVEACGICRT
                     DLHIVSGELPPRLPAVIPGHQIVGRVEALGRDVTQWSKGDRVGVSWLGGTDGSCLLCR
                     KGMENLCDHPVFTGYTVNGGYAEYAIARADFCIPLPENASAIELAPLLCAGIIGFRSL
                     RVADVQPGDRVGLFGFGASAHLAIEVLKHWKCEVYVSTRGTSHQQLAHELGATWVGDA
                     TQRPPVPLDRAVTFAPSGDIVIAALASLRKGGIVGINAIHLDRMPQFDYDTLLWGERQ
                     LRSVTNMTRQDAHDFAKLAGEIGIRPRATVFTLDQVNEALAAVHHDAVNGAAVIKMDD
                     A"
     gene            complement(2884..3639)
                     /locus_tag="VFA02_13645"
     CDS             complement(2884..3639)
                     /locus_tag="VFA02_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZU10942.1"
                     /translation="MAALRILIVDDEPAIRSGIRRMLSSVPDAEVIAECANAAEAIEA
                     IQHGEANLVLLDIHLPDASGLEVVRTVGPERMPPTVFVTAYDEHAIEAFELNALDYLL
                     KPLDEERLERALARAREKLASKQPAQLHQLEALLASYRPAGPQRFVVRKSNHYEFVSR
                     ESIDWVESADNYVELHCGGKTHLLNETMGRMEQLLDTKKFLRVHRRYIVNLDRIQAIH
                     PLAGGAYEIEMQHGARLTSGRNYAGAIRELLGR"
     gene            complement(3608..4786)
                     /locus_tag="VFA02_13650"
     CDS             complement(3608..4786)
                     /locus_tag="VFA02_13650"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU10943.1"
                     /translation="MADVETLSRVADVETLSRRQSGVRYPGWLALFCIWTLIGVLAFA
                     RHYLDTSAAGRHEFSLKELAEWLTCFYPWVLFAPVVFEMERRFPLNRQQWKRSVPVLF
                     VFALAVSFAVMKCTQILCLILNRKADLHAVLYTLSPGEWVLAFFLFWNVAAAGAIIRT
                     FIHLERQQRVAAELALENAKLETSLKNAELEALRLRLKPHFLFNTLENISALAEDNPK
                     LARRMLARLGELLRAAVRRDYQAEIRLDAEIALMNAYLDVERVRFGERLHVDVEVAPE
                     TETALVPSLLLQPLVENAIQHGLRNVSGAGWIEVTSRKSSEYLVLTIKDNGSGPPDEM
                     ALGIGLGSTRERLMRMYPQEHRLFIEKLQNGGTLVYIQLPFRTRNASSNGSAAHSYRG
                     "
     gene            4938..6095
                     /locus_tag="VFA02_13655"
     CDS             4938..6095
                     /locus_tag="VFA02_13655"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZU10944.1"
                     /translation="MTVAGIMSGTSADGMDVALVRVRPQKSRLKLDLLAHEAFPFPKD
                     LRAAVLAAMDAKSISTAELARLNWRLGLAYAEAVRETLKRHSYKLDLIGCHGQTIYHQ
                     GTPARFAGKKFACTWQIGEAALIAAEMHVPVISNFRPADMAAGGQGAPLVPLLDYVQF
                     ANQKRARVLQNIGGIGNLTAIPPASGIEKILAFDTGPGNMVIDALMQQLFGRPYDRGG
                     RIAASGNVLHPVVESVLRQPFFLTRPPKSAGREEFGASFAEDFLRECRKTGGSEKDAI
                     ATATALTAQSIALAFTRFVQPRLQRGAPVDYIVSGGGAKNTTLMRMLREKLGPLGCTL
                     LDSSALGLPVEAKEAAAFALLAYYTWHRRPANVPSATGAKHPAILGQITYV"
     gene            6088..7671
                     /locus_tag="VFA02_13660"
     CDS             6088..7671
                     /locus_tag="VFA02_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU10945.1"
                     /translation="MFKNSPAMPETPGAPCLKETRAIYKLLIVIVFACLLSGCNHDRP
                     PGNTVTVLIESSPTNLDPRIGVDAQSERIDSLIFDSLVRKDEHFDLQPWLAEKWDSPN
                     PLTYIFHLRDGVHFHDGRPLTSADVKYTLDSILQGKVISVKANAYQTIDHIEAPDRLT
                     AIIHLKKPDPALLWNLSDGGFGVVPAGSGRDFWRHPIGSGPFEFVSQEQDKDVIIERA
                     PHSWQTQPAIERVRFAVVPDATTRALELQKGSADAAINALPADTVYALRHERNLQIAS
                     GPGTTLMYLIFNTRDPVLQDARVRRAIALAINRPLLIHSLFRDQARLAESVLPPEHWA
                     WDGNVPPHHYDPAAANKLLDEAGYPRKSDGFRFHIGMKTSTDEGSRLLAMVIQQQLSQ
                     IGIAVDLRSYEFATFYADISRGIFQLAPSRWIGGNEAPDIFRYSFATSSFPPHGANRG
                     FYANPEIDRLLDDAASGVDQARQKEDYARVQEILARDLPTINLWYLDTVLVHSRRLQN
                     IHLSPSGNYDFLRDATLKE"
     gene            7682..8707
                     /locus_tag="VFA02_13665"
     CDS             7682..8707
                     /locus_tag="VFA02_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HZU10946.1"
                     /translation="MKALLLSQYKKLEISDLPRPVPGPGEVLVKVAACGICGSDVHGY
                     DGSSGRRIPPIVMGHEAAGVVAETGANVTGFRPGDRITFDSTVYCGECEYCRRDEVNL
                     CDNRQVVGVSCEDYRRNGAFAEYIAVPQRILYHLPDTLSFPEAAMLEAVSVALHAVRL
                     AELKGEETALVIGAGMIGLLTLQAARALGASRVIVTDVDATRLKLAKEMGADETVLSG
                     PNGNFAQIADVAFEAVGRSETIAAAIDGVRKGGTVVLIGNITPQVTLPLQKVVSRQIR
                     LQGTAASAGEYPQAIELMTNGHVRVKPLISAVAPLEEGPGWFTRLYAREPNLMKVVLT
                     PANEVTQ"
     gene            8704..9501
                     /locus_tag="VFA02_13670"
     CDS             8704..9501
                     /locus_tag="VFA02_13670"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU10947.1"
                     /translation="MSALFDLTGQVAIVTGTSRGLGQYFARALAKAGADLVLTSRKRE
                     TLADFEAEIKSLGRHVLPLELDVRDHASIERMAKEAEAAFGQIHILVNNAGCNVRKPA
                     LDVTWDDWNLVLDTNLRGSFFVAQAVARNMVRHGYGRIINIGSVTSVAGYAGLGPYTA
                     SRGGIRQLTMSLADDWGRYGVTVNCLAPGWFRTAQNKILYEDPQWVEYLTDRIPLKRP
                     GSPDDLDGAVVFLASESSRYITGQTLLVDGGISTGAVRATVSTKDKP"
     gene            9498..>10283
                     /locus_tag="VFA02_13675"
     CDS             9498..>10283
                     /locus_tag="VFA02_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU10948.1"
                     /translation="MNKARKLIVWCGLLAVSVAVTFAQNSKDLRVYVIDVEGGQATLF
                     VTPAGQSLLIDTGWPGQNGRDADRIVAAAKKAGISKIDYVLITHYHTDHVGGVPQLAE
                     RIPIGTFIDHGPNYETSDKATEEGYNAYQNLLATGKYKHIVPKPGDVLPIQGMKVLVI
                     SSNGDLIDHSLPGGGEQNSFCKVSESRPADKTENSHSLGVLVTFGKTRILDLGDLTWD
                     KEMQLMCPVNRLGHVPILIVSHHGWYQSSSPALVDAIHPQVAIM"
BASE COUNT         2352 a         3050 c         2732 g         2149 t
ORIGIN      
        1 atttattttg aagaatttac agaatttact actcacccta ctcttctccc ttccagacgt
       61 acgcatcagc ctgcggaagc ccgatgtgcg ccagcacacg ggccacaaac tgcttcgcca
      121 tggctgtgga atccaccgga tggttataga aggttggaat gacgggaaag atcgtggctc
      181 ccgcatcgga agccagagac atattgcgca gatgaatttt gttgaacggg gtctctctta
      241 cgcaaagcac cagaggccgc cgttctttca ggcaaacatc ggctgcccgc tcgacaaggt
      301 tctgggccag accatgggca atcccggcca gtgttcccat gctgcatggc agaataatca
      361 ttccgttgga tggatagctg ccgcttgcaa tcggcgctcc gatgtccgac tcagcgtgct
      421 gcaggatttt agaggaactc tgtccaggca gcttctcaac cagagcattg cgcccgctga
      481 gtccaagctc ctcggcaatg acccgcagcg cgctttctga aaccacaaga tgcacgcggt
      541 caacacgggc atctgcctcc agtgcctgca ataactgctg cccgaagacg gacccgctgg
      601 ctccggtgat ggcgacagta aggttgagat gattttccat tgttctattg tcgccatcct
      661 tgtgtctgct tcgctaaatg tttgtcatcc tgagcgaagg acctgctttt cttttcaggc
      721 tgtacgttat cttaaagcga attatgccgc attcgcaggc ttcggtctgt tccacatacg
      781 gcgtatgaaa cgcgccagcc agcgcaccgt gatgaccgcc agtatcagaa agatgacggc
      841 aatcgttgcc gctacaaaag gatgccgcgt cgccagccac gtaaccccaa tggccacgac
      901 gtcctcactc gtgctgagcg cgatattgga gaccggctcc ggcgtaggtg ttacggccgc
      961 cctcaccgca gtcttcgatc cgtgggaaac aatcgcgatg cccgctcctg caacggctgc
     1021 aagaagctgc atctccggcg atagctgttc gctggcacga tacgcaagca gcgccgcaac
     1081 aggaacgcgc acaaatgtat gcagcgcgtt ccagatcatg tcaaagccgg ggactttatc
     1141 ggcgaagaac tcgatcacaa acatcgcgcc gctcacgccg atcacccacc agttgttcag
     1201 aatgtccaga cccgcaggca atgcgatcca atgcacgtgg gccagaagcc caagcgtcag
     1261 cacagtggca tacacattca accctgcggc aaaactggct gcgataatca gggcggcgat
     1321 ggtagtgggt gtgaatgaca tgacttacct gagcgccgaa cagcttgccg gatacttctg
     1381 cccgttcacc accacccatc cattatctgc accacggtgt gtcgagcaat gaacgtcatg
     1441 aattccggca acaaaatgcc cacgcgatgg aaagaacgcc tgaccgtaag catagacttg
     1501 tgcatttaca ggagcggtaa ccacgccatc caggtcatcg tttgtcacta cctcgaccag
     1561 gcgcgtgctg ccgtcaggaa atgtcacacg cagcggaaga atctcatggt ttccgtgcgg
     1621 acctccgcgc tgcgtgcgcg aggaactgac cgtgatgccg caaaccatta cattgtccac
     1681 agcctgcgtc tgatttgcat gcagcagatc gcccaacttc tgattgacct ggggagtgca
     1741 gctctgcggc acagaaacat ccttctgcgc caacgctgct gatagcgaaa aagcagcaaa
     1801 catcaggaac cggacaacgt gtcgttcttt tcgcattcgt tgagcatact acagcagaat
     1861 gagggattac gcatcatcca ttttgattac ggcagcgccg ttgaccgcat cgtgatgcac
     1921 cgccgccaaa gcttcattca cctgatcgag agtaaaaacc gtggcccgcg gccgaatgcc
     1981 aatttctcca gccagttttg caaaatcatg cgcatcctga cgggtcatat tggtcacgct
     2041 gcgcaactga cgctcgcccc acagcagcgt gtcgtaatcg aactgcggca tgcggtcaag
     2101 atgaattgcg ttgatcccaa caatcccacc ttttcgcagt gaggccagcg cagcaataac
     2161 aatatctccg ctgggagcaa atgtcactgc acggtcaagc ggtacaggcg ggcgctgagt
     2221 ggcatcaccc acccaggttg ctccaagctc atgggcaagc tgctggtgcg atgttccgcg
     2281 agttgagacg tagacttcgc atttccagtg cttcagcact tctatcgcca gatgggccga
     2341 cgcgccaaaa ccaaagagtc ccacgcggtc tcccggctgc acatctgcca cgcgaaggct
     2401 gcgaaatccg ataattcccg cgcagagcaa tggcgcaagc tcaatggcag acgcgttttc
     2461 aggcaatgga atgcagaaat ctgcgcgggc aattgcgtac tccgcatagc cgccgtttac
     2521 ggtgtatccg gtgaagacgg gatgatcgca gaggttttcc attcctttgc ggcaaagcag
     2581 acaggaaccg tccgtgcctc caagccacga aacaccaaca cggtccccct tgctccattg
     2641 cgtaacatcc cgacccaaag cctctacccg cccgacgatc tggtgcccgg ggataacagc
     2701 aggcagccgc ggaggcaatt ctccactgac aatatgcaga tcggtgcggc agatcccgca
     2761 cgcttcaacc ttgagcagga cttcgcctgc cgcgggctgc aactccggga cttcttccac
     2821 ttggagtggc ttctgctcaa ctggtgcggt gtggcgaagg actgcggcac gcatgcgacg
     2881 attctaccgc ccgagcagct ctctgatggc accagcgtaa ttccttcctg aagtgagtcg
     2941 tgcgccatgc tgcatttcaa tttcataagc accgccggcc agcggatgaa tggcctggat
     3001 ccggtcaaga ttgacaatat accgccggtg aacccgtaga aacttcttcg tgtcgagaag
     3061 ctgttccatc cggcccatgg tctcattcag caggtgcgtt ttcccgccgc agtgcagctc
     3121 cacataattg tcagcggact ccacccaatc aatgctttct ctggagacaa attcataatg
     3181 atttgacttg cggacaacga accgctgtgg ccctgcagga cggtaagacg ccagcaatgc
     3241 ctcaagctgg tgcaactgcg ctggctgttt cgaggccagt ttttccctgg ctcgggccag
     3301 agcacgctcc agacgttctt catctaacgg tttaagaaga taatccagcg cattcaactc
     3361 aaaagcttca atggcgtgct catcgtaggc agtcacaaac accgtgggag gcatccgttc
     3421 aggcccgacg gttcggacaa cttcaagtcc ggaagcatcc ggcaagtgaa tgtcgagcag
     3481 cacaaggttg gcttctccat gctggatggc ctcgattgcc tccgccgcat tggcgcattc
     3541 tgcaatgact tcggcgtccg gcacagacga tagcattctg cggatgccgc tgcgaatcgc
     3601 cggctcatca tccacgataa gaatgcgcag cgctgccatt ggaagacgca tttcgggtac
     3661 ggaaaggaag ctggatatag acaagggtcc ctccattctg gagtttttca atgaaaagcc
     3721 ggtgttcctg cggatacatc cgcatcagcc gctcgcgcgt cgagcccagg ccaatgccca
     3781 gcgccatttc atccggaggt ccgctgccat tgtccttaat ggtcaggaca agatactcgg
     3841 aactctttct gctggtgact tcaatccacc cggccccgga aacgtttcgc aatccatgtt
     3901 gaatggcatt ttcaaccagc ggctgcaaca acaggctggg cacaagcgcg gtttccgttt
     3961 ccggagcaac ttccacgtct acatgcaggc gctcgccaaa gcggacgcgc tcgacatcaa
     4021 gataagcatt catcagcgcg atttcggcat ccagtctgat ctccgcctgg taatcacgcc
     4081 gcacggcagc tcgcagcagc tcgccgagac gcgccagcat cctgcgcgcc agtttgggat
     4141 tgtcctccgc aagcgcagag atgttctcaa gcgtattgaa aagaaaatgc ggcttcaggc
     4201 ggagccgcag cgcctccagc tctgcgttct tcaggctggt ctccagcttt gcattttcca
     4261 gtgcaagttc tgcggccacg cgctgctgcc gctccagatg gataaacgtg cgaataatgg
     4321 cccccgcggc ggccacgttc cagaagagga aaaaagcgag tacccattct cccggtgaaa
     4381 gagtatagag taccgcatgc aaatcggcct tgcggttgag aatcagacac aatatctggg
     4441 tgcatttcat aaccgcaaag gacaccgcca gcgcgaatac aaacagcaca ggcacgctgc
     4501 gcttccactg ctgccggttc aacggaaagc gccgctccat ttcaaaaaca acgggcgcaa
     4561 aaagtaccca gggatagaag caggtcagcc actcggccag ttctttcaat gaaaattcgt
     4621 gcctgcctgc tgcgcttgta tccagatagt ggcgtgcaaa ggccagcacg ccaatcagcg
     4681 tccagataca aaacagcgca agccatcccg gatagcgcac tccggactgt ctgcgtgata
     4741 aggtctctac gtccgccaca cgtgataagg tctctacgtc cgccacaata gtatcctcgg
     4801 atgattgcat cctacgccgg agcaaaattt gggccaagcg gtggatattt tgggtaaacc
     4861 gcatccagga acggagtcat tacaatgtgt tctgtagctc cgaggcattt ctattgaaac
     4921 cacttccctt ccgaaccatg actgtcgccg gcatcatgag cggtacttcc gccgatggca
     4981 tggatgttgc tctggtgcgg gtccgtccgc aaaaaagcag gctgaaactc gacttgctcg
     5041 cgcatgaggc gtttcctttc ccaaaagatt tgcgtgcagc agtactggct gcaatggatg
     5101 caaagtccat ctcaaccgca gaactggccc gtctcaactg gcgactcggg cttgcctatg
     5161 cggaagccgt tcgagaaact ctgaagcggc attcgtacaa actcgacctg attgggtgcc
     5221 acggccagac catctaccat cagggtacgc ccgcccgctt tgcaggaaag aaattcgcct
     5281 gtacatggca aatcggcgaa gccgcgctga ttgcggcaga aatgcatgtc ccggtcatca
     5341 gcaatttccg cccggccgat atggcagcag gcggacaagg cgcgccgctc gttcctctgc
     5401 tggattacgt gcagttcgcc aaccaaaaac gggcgcgcgt gttacagaac atcggcggca
     5461 ttggaaacct gacggcgatc cctccagcat ccggaataga gaaaatattg gccttcgaca
     5521 ccggcccggg gaacatggtg atagatgcgc ttatgcaaca gcttttcgga agaccttacg
     5581 accggggcgg aagaatcgct gcgagcggaa acgttctgca tccggtcgtg gaatcggtct
     5641 tgcggcaacc cttctttttg acaaggccgc caaagtccgc aggacgcgaa gagttcggcg
     5701 catcttttgc agaggatttt ttacgtgaat gcagaaagac cggcggttca gagaaagatg
     5761 ctatagccac ggcaacggcc cttaccgcgc aaagcatcgc gcttgctttc acacgtttcg
     5821 tgcagccgcg gctccaacgc ggcgctccgg tggactacat cgtctccggg ggtggcgcaa
     5881 aaaatacaac tctgatgcgg atgttgcgcg aaaagctggg accgcttggc tgcacgctgc
     5941 tcgattcgag cgcattgggc cttcctgtcg aagcaaaaga ggccgccgca ttcgctttgc
     6001 tcgcctacta cacatggcac agacgtcctg caaacgtacc ctccgcaacc ggagcaaagc
     6061 atccggctat tctggggcag atcacgtatg tttaaaaact cccccgcaat gccggagact
     6121 ccgggtgccc catgtctcaa agagacacgg gcaatttaca agctgctgat cgtcatcgtc
     6181 ttcgcctgcc tgttgtccgg ctgcaatcat gaccggccac cgggcaatac cgtcaccgta
     6241 ctgatcgaaa gcagcccgac gaatctcgac ccgcgcatcg gcgtagatgc gcaatccgaa
     6301 cgcattgatt cgcttatctt tgattcgctc gttcgtaagg atgagcactt tgacctgcaa
     6361 ccctggcttg ctgaaaagtg ggactctccc aatccgctga cttacatctt tcatctgcgc
     6421 gatggcgtgc attttcacga cggccgccca ctcacatcgg ctgatgtgaa gtacacgctt
     6481 gattcgatcc tgcaaggcaa ggtcatcagc gtgaaggcga atgcctatca gaccattgac
     6541 cacatcgaag ctccggaccg ccttacggcc atcattcacc tgaaaaaacc agacccggcc
     6601 ctgctgtgga acctgagcga tggtggattc ggcgttgtgc ccgcaggcag tggccgcgat
     6661 ttttggaggc accccatcgg cagtggtccg tttgagttcg ttagccagga gcaggacaaa
     6721 gacgtcatca tcgaacgtgc gccgcattcc tggcagacgc agcctgcaat cgaacgtgta
     6781 cgctttgccg ttgtccccga tgcaacaacc cgcgcgcttg aattgcaaaa aggctcggca
     6841 gatgcagcca tcaatgctct tcctgccgat acggtctatg ctctacggca cgagcgcaat
     6901 ttgcagatcg cctccggccc gggcactacg ttgatgtatc tcatcttcaa cacgcgcgac
     6961 cctgttcttc aggatgcccg tgtgcgccgc gccattgctc tggccatcaa ccgtcctctg
     7021 ctgattcact cgctcttccg cgaccaggcg cgtctggcag aaagcgtgct tccaccggag
     7081 cactgggcat gggacggcaa tgttcctccg catcattacg atcctgccgc agcaaacaaa
     7141 ttgctggacg aagcgggcta tccgcgcaaa agcgatggct tccgcttcca cataggcatg
     7201 aagacctcga ctgacgaagg cagccgcctg ctggctatgg tcatccagca gcagctttca
     7261 cagataggca tagcagtaga cctgcggagc tatgaattcg ctacttttta cgccgatatc
     7321 agccgcggca tctttcagct tgcgccttcg cgctggattg gcggaaatga agctccggat
     7381 atttttcgtt attcatttgc cacatcgagc tttccacctc atggcgccaa ccgcggtttc
     7441 tatgcaaatc ctgaaataga ccggctgctc gacgacgcgg cttcaggcgt agaccaggcc
     7501 cggcaaaaag aagactacgc gcgtgtgcag gagattctgg cgcgcgacct gccgaccatc
     7561 aatctttggt atctggatac agttcttgtc cacagccgca gattgcagaa catccatcta
     7621 tcaccatcgg gcaattatga ttttttgcgc gatgcgaccc tgaaggaata aaacggtagt
     7681 gatgaaggcc ctgctgcttt cccaatacaa gaaactcgaa atctctgacc ttccccgccc
     7741 tgtgcccggc cccggtgagg tgctggtcaa ggtcgcagcc tgcggcatct gcggaagcga
     7801 tgtccacggc tatgacggtt catccgggcg ccgcatccct cccattgtga tggggcacga
     7861 agccgcaggt gttgtcgcgg aaacaggcgc aaatgttact ggattcaggc ccggtgatcg
     7921 catcaccttc gactccacgg tctactgcgg cgagtgtgag tattgccgcc gggatgaagt
     7981 caatctctgt gacaatcgtc aggtcgtcgg cgtttcctgc gaagattacc gccgcaatgg
     8041 cgccttcgcc gaatacatcg ccgtcccgca acgcattctc tatcatctgc cggataccct
     8101 ctcttttcct gaagcggcga tgctcgaagc agtctccgta gcacttcatg cagtgcgtct
     8161 ggcagaactg aaaggcgaag aaactgcgct cgtcatcggt gcaggcatga tcgggctgct
     8221 cacgttacag gcagcacgcg cgctaggcgc atcacgtgtg attgtcaccg atgttgatgc
     8281 aacccgtctg aaactggcga aagaaatggg cgcggacgaa accgtgctct ccggcccgaa
     8341 tggaaacttt gcccaaattg cggatgttgc gtttgaggct gtaggccgaa gtgagacaat
     8401 tgccgcagcc attgacggag tgcgtaaagg cggtaccgtt gtcctgattg gaaacatcac
     8461 gccgcaagtc acactgccat tgcagaaagt agtctcgcgg cagattcgct tgcaaggcac
     8521 agcggcctca gcaggagaat atcctcaggc catcgagttg atgacaaacg gccatgtccg
     8581 tgtgaagccc ctgatttcag ccgtcgcccc tctcgaagaa ggccctgggt ggtttactcg
     8641 cctttatgcc cgcgagccaa atctgatgaa ggtcgtcctc actcccgcaa atgaggtcac
     8701 gcaatgagcg cattatttga tctcaccggc caggtggcca tcgtcaccgg gaccagccgc
     8761 gggcttggac agtacttcgc gcgcgcattg gcaaaggccg gagccgatct cgtcctcacc
     8821 agccggaaac gtgaaacgtt ggcagatttc gaggccgaga ttaagtctct gggccgccac
     8881 gtgctcccgc tcgagcttga cgtgcgcgac catgccagca ttgagcgtat ggcaaaagaa
     8941 gccgaagctg cttttggaca gattcacatt ctcgtcaaca acgcaggatg caacgtacgc
     9001 aaacccgcgc tcgacgtcac ctgggatgac tggaaccttg tgctggacac gaacctgcgc
     9061 ggcagctttt ttgtcgccca ggctgtcgca cgcaacatgg tccgccacgg ctacggccgt
     9121 atcatcaaca ttggttcagt cacaagcgtt gccggatacg ccggtctggg gccatatact
     9181 gccagccgcg gaggaatccg ccagcttacg atgagtcttg ctgatgactg gggcagatac
     9241 ggagtaacgg taaactgcct cgctccagga tggttcagaa cagcgcaaaa caaaattctc
     9301 tatgaagacc cgcagtgggt cgagtatctg accgaccgta ttccgctgaa gcgtcccgga
     9361 tctccggatg atctggatgg cgcggtcgtc tttctcgcct ctgaatcgag ccgctatatc
     9421 accgggcaaa cgctcctcgt agacggaggc atttcaaccg gcgccgtgcg cgcaaccgtt
     9481 tccacaaagg acaagccatg aacaaagcac gaaagctgat cgtatggtgc ggcctcctgg
     9541 ccgtaagtgt agctgtcact tttgcgcaga acagcaaaga tttgcgcgtc tacgtcatag
     9601 atgtggaagg cggacaagct actctgtttg tcacgcccgc aggccagtct ctgctgattg
     9661 acaccggttg gcccggtcaa aacggtcgcg atgccgaccg cattgttgcc gctgcaaaaa
     9721 aagccggcat cagcaaaata gattacgtgc tgattacgca ctatcacaca gaccacgtgg
     9781 gcggcgtacc gcaactggct gagcgcattc ccatcggcac gttcattgac cacggcccaa
     9841 actatgaaac atcagacaag gccacggaag aaggctacaa tgcctaccag aatctgctgg
     9901 caacaggaaa gtacaagcac atcgtcccaa agccgggaga tgtattgccc attcagggga
     9961 tgaaagtact ggtcattagt tccaatggcg atctgattga ccattctctg cccggcggcg
    10021 gagaacaaaa ctccttttgc aaagtttcag aaagccgccc tgctgataaa acagaaaact
    10081 cgcactcgct cggcgtcctt gtcactttcg ggaagacacg cattctcgac ctcggcgacc
    10141 tcacctggga caaggaaatg cagcttatgt gccctgtgaa caggcttggc catgtcccca
    10201 tcctcatcgt ctcgcaccac ggatggtatc aaagctccag cccagcgctg gtagacgcca
    10261 tccatccgca ggtcgccatc atg
//
LOCUS       DASRBV010000372         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_137985, whole genome shotgun sequence.
ACCESSION   DASRBV010000372 DASRBV010000000
VERSION     DASRBV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137985"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..402
                     /locus_tag="VFA02_13680"
     CDS             142..402
                     /locus_tag="VFA02_13680"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZU10949.1"
                     /translation="MEEVVTIATFTEPLEAEMVRLRLESAGIETFLAGENARIMEPGL
                     GPLQLQVKAADEDDARAILADRGDENSGDENASGPSTGPDMS"
     gene            431..943
                     /locus_tag="VFA02_13685"
     CDS             431..943
                     /locus_tag="VFA02_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZU10950.1"
                     /translation="MVPVTQPQAMAVSRKAPATETKGTGLSVEQFQRLAEFRFQLRRF
                     LHFSHLAAESAGVRHQQYQLLQCVYGMPGELDPTIANVAARMLLKHNSAVELVDRTIE
                     QGLLRRCPDPTDHRRILLRVTPLGERILASLADYHLQELEQAGPELIRALRRVVSMKQ
                     ISNGKRPTTQ"
     gene            940..2823
                     /locus_tag="VFA02_13690"
     CDS             940..2823
                     /locus_tag="VFA02_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZU10951.1"
                     /translation="MSAPGTTGHTDRNISAFRSHLRDFTADPRMLVLSGLALILGAAG
                     AALAYLLLHLIYVATNLFYFHRLSWQFISPAANRLHWLAIFIPVIGGIIIGFMARYGS
                     DKIRGHGMPEAIEAILINGAKVDGKVAVFKPLSAAIAIGSGGPFGAEGPIIMTAGSVG
                     SLIGQLFRLSDAERTTMLVAGAAAGMTGVFSTPLSAVLLAVELLLFEWRPRSLVPVAV
                     AATTAGVLRRHLLGPGPLFPMPLYSMTVGPRMVVAALILGVVGGVAALLLSRAVYASE
                     DFFEEHLPVHWMWWPAIGGVVIGIGGLIFPRALGTGYDVIDSLISGNTAWQLIAGVLI
                     VKSIIWAFSLGSGTSGGILAPLLMIGGALGALLGHGLPFIQVGAWPLVGMAAVLSGAI
                     GCPLTAAALSMELTHNYGLMLPLLAASVAAHAFTVLFQKRSILTERLSRRGYHLSREY
                     SVDPLEIMTVSQVMRSSVVALPVDARIADAKRWIEDRRSPDKMPEAGDMRSRRGQRLY
                     PVVEANGKLAGIVTRRMLEYFAESHPPDAPLPFTAAEVAYPDETLRAVAERMAALRVF
                     ALPVVEPGTGKVTGILSLEDMLQARFRSHERETRLERVRRLRMPFRGERIGQAEESLE
                     TTP"
     gene            complement(2970..3230)
                     /locus_tag="VFA02_13695"
     CDS             complement(2970..3230)
                     /locus_tag="VFA02_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZU10952.1"
                     /translation="MRVFLYSAAWCRDCREAKRFLEQHGISYTEIDIESVDGAAEEVF
                     RHVGKRAIPQLVIDGKWFQPYRPGQGLLYQELSEVLGITIPN"
     gene            complement(3274..3639)
                     /locus_tag="VFA02_13700"
     CDS             complement(3274..3639)
                     /locus_tag="VFA02_13700"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HZU10953.1"
                     /translation="MNDRKMESLIGQLLRFGVALAALVVFAGGVLYLHQSRGPRPDYA
                     AFHGESPALRSPSGILQELPSANSNAIIQLGLLLLIATPVARVIFAAIGFSLERDWFY
                     TAVSLIVFAVLMYSLLADH"
     gene            complement(3636..>3893)
                     /locus_tag="VFA02_13705"
     CDS             complement(3636..>3893)
                     /locus_tag="VFA02_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU10954.1"
                     /translation="PFKVSTTTSNFMIGVTAAASAGIYLSRGYISPGLAMPVMLGVLL
                     GSLAGSRILVKAKVKTLRLVFATVIVLLGMEMIYNGMTGRL"
BASE COUNT          796 a         1029 c         1188 g          880 t
ORIGIN      
        1 agtgaaggcc gcggagaaaa cctgcttcgc ttttgctttg ccaagaagga cgaagacctg
       61 ctgcgtgcct gcgagctgct gagaaagttt cagacatgaa gtggtgaata ctcgtgatga
      121 acggggacaa cggggaattc catggaagaa gttgttacga ttgctacctt tactgagccg
      181 ctggaagcgg aaatggtgag gcttcgtctt gaatctgccg gaattgaaac ctttctggcc
      241 ggcgagaacg cccgcattat ggaacccggt ctggggccgc tgcaattgca ggtaaaagcg
      301 gcggatgaag acgatgccag ggccatcctt gctgatcggg gagacgaaaa ttctggggat
      361 gagaatgctt ccgggccatc tacaggtcca gatatgtcgt aatgcgagat aatcgtgata
      421 cgatatattt atggtccctg tcacccaacc gcaggcgatg gctgtttcgc gcaaggcacc
      481 agccacagag acgaagggga ccggcctcag cgttgagcaa tttcagcgcc ttgctgagtt
      541 ccggtttcag ttgcgccgtt ttctgcactt cagccatctg gccgcggaaa gcgccggcgt
      601 ccggcaccag caatatcaat tgctccagtg cgtgtacggg atgccgggcg agcttgatcc
      661 gacgatcgcc aatgttgcgg cgcgcatgct tttgaagcac aacagcgccg tagaactggt
      721 ggaccgcacg atcgagcagg gactgctgcg ccgctgtcct gatccgacag accatcgccg
      781 cattctgctg cgcgtgactc cgctggggga gcggattctg gcttcgcttg ccgattatca
      841 cttacaggaa ctggagcagg ctgggccgga actgattcgc gccttgcgga gagtagtgag
      901 catgaaacag atttctaatg ggaaaaggcc tacaacgcaa tgagtgctcc tgggacgacc
      961 gggcatactg accgaaatat ctccgctttt cgctcgcatt tgcgcgactt taccgccgac
     1021 ccacgcatgc tggtactgag cggtctcgcc ctgatactgg gcgcggccgg cgcagcgttg
     1081 gcttatctgt tgctgcattt aatttatgtt gcgaccaacc tgttttactt ccaccgtctg
     1141 agctggcaat ttatttctcc ggcagcaaac cgcctgcatt ggcttgctat cttcattccc
     1201 gtcattggcg gtatcatcat tggctttatg gcccgctatg ggtcggacaa aatccgcggt
     1261 catggtatgc cggaggcgat cgaggccata cttatcaatg gcgcaaaagt ggatggcaag
     1321 gttgctgtat tcaagccgct ctctgctgcg attgcaatcg gttcgggtgg tccgtttggc
     1381 gccgaaggcc cgatcatcat gacggccggg tcggtcggtt cgctgatcgg gcagctcttc
     1441 aggttaagcg atgcggaacg cacgaccatg ctggttgcag gcgcggcagc aggcatgaca
     1501 ggggtctttt ctactccgtt gtccgctgtg ttgctggcgg tagaactgct tctctttgag
     1561 tggaggccgc gcagccttgt tccagtggct gtggcagcta cgactgccgg agtgctgcgg
     1621 cggcatctgc ttggcccggg gccgctcttt cccatgccct tgtatagtat gacggttggg
     1681 ccgcgcatgg ttgtagcggc gttgatatta ggggtggtgg gtggagtggc agcgttgtta
     1741 ttgagccgcg ctgtatatgc cagtgaggac ttttttgaag aacacctgcc tgtgcactgg
     1801 atgtggtggc cggccatcgg tggcgtggtg attggcattg gcggtctcat ttttccgcgc
     1861 gctctgggca cgggctacga tgtgattgat tcactcatct ccgggaacac ggcctggcaa
     1921 ctgattgcgg gcgtgctgat tgtcaagagc attatctggg cgttttcact ggggtccgga
     1981 acatctggag gcattcttgc tccgctgctc atgataggcg gcgcgcttgg cgctttgctt
     2041 ggacatggcc tgccatttat tcaagtcgga gcatggcctc tggttggaat ggcggcggtg
     2101 ctctctggtg ctattggttg tccattgacg gcggctgctc tgagcatgga gctgacgcac
     2161 aattatggcc tgatgcttcc attgctggcc gcatcggttg cggcgcatgc ctttacggtg
     2221 ctcttccaga agcgctcgat ccttacagag cgcctaagcc gccgcggata tcacctgagc
     2281 cgcgaatata gcgtagatcc actggagatt atgacggttt cgcaggtgat gcgctcaagc
     2341 gttgtggccc tcccggtaga cgcgcgcatt gcggatgcaa agcgctggat cgaagaccgg
     2401 aggtctccgg acaagatgcc ggaagctggg gacatgcgaa gccggcgtgg acagaggctc
     2461 tatcccgtag tagaggcgaa tggaaagctt gcaggtattg ttacccggcg aatgctggag
     2521 tattttgcag aatctcatcc gccggatgct ccgctgccat ttacagcggc agaggttgcg
     2581 tatccggacg agacgcttcg tgctgttgcc gagcggatgg ccgcattgcg cgtgtttgcg
     2641 cttcccgtag ttgagccggg gacaggaaaa gtaacaggta ttctttcttt ggaagatatg
     2701 ttgcaggccc gcttccgctc gcatgagcgc gaaaccaggc tggaacgtgt gcggcggctg
     2761 cgaatgccgt tccgtggaga gcgcatcggg caggccgagg aaagcctgga aaccacgcct
     2821 taatgcattt ttagtttcac cgtggtccac gaaaaggctg gtcgtcattc cgggcgaagc
     2881 agaaaacctt agcagaattg gctacggcat ttttacagat tttgtacaat ccatttgcac
     2941 tgtgcagtgc cgccggctcc ctgtgccagt caatttggga tggtgatgcc aagcacttcg
     3001 ctcagctcct ggtacaggag tccttgcccg gggcggtatg gctggaacca ttttccgtca
     3061 atcacaagct gcgggatggc gcgcttgcca acgtggcgga acacctcttc cgcggcccca
     3121 tccacgcttt caatgtcaat ttcagtatag gaaatgccgt gctgctcaag aaagcgcttg
     3181 gcctcgcggc aatcgcggca ccacgccgca gaataaagaa agacccgcat ggccgatatc
     3241 ataccgggag cggcatacat ttggcatctc cggtcaatga tctgccagga ggctgtacat
     3301 cagaacggcg aacacaatca ggctgacggc ggtgtaaaac cagtcccgct ccagcgaaaa
     3361 accgatggcc gcgaagatga cccgggcaac cggcgtggca atgagcagaa gcaagccaag
     3421 ctgaatgatg gcattgctgt tggccgaagg cagttcctga agaatacccg aagggctgcg
     3481 cagggccggc gattcaccat ggaaggcagc gtaatcgggg cgcgggccgc gcgattggtg
     3541 caggtatagc acgcctccgg caaaaacgac gagcgctgcc agggccactc caaaacggag
     3601 caactggccg ataagcgatt ccattttcct gtcgttcata gccgtcccgt cattccgttg
     3661 taaatcattt ccatgccgag aagaacaatc acagtggcaa agaccagccg cagagtcttt
     3721 acctttgcct tcaccagaat gcgagatccg gccagcgaac ccagcagcac gcccagcatg
     3781 acaggcatgg ccagtcccgg agaaatgtaa ccgcgactga gataaattcc cgcgcttgct
     3841 gctgccgtta caccgatcat gaaattgctt gtcgtagttg agactttgaa ggg
//
LOCUS       DASRBV010000373         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_600983, whole genome shotgun sequence.
ACCESSION   DASRBV010000373 DASRBV010000000
VERSION     DASRBV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600983"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VFA02_13710"
     CDS             complement(<1..1523)
                     /locus_tag="VFA02_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZU10955.1"
                     /translation="MEQTAYGPVPIRHSFVHEKGIFRFSLISGALIFLAFTGLAIWLL
                     PFSVPTQVSVLMHTLLGLAVVIPLTIWQLSHWLATRKARRSFRKICAYTGFWTMAVVS
                     ASGLVLTWQAMFGLYTSRFWDQVHLWCGIAALPFLGYHVWPRAKKAANGLNNGLNGLK
                     GNGGSVLPDFRPGRRHMWLISAVTAVVLLATVGVIAGIYRAYAPNFSHYSLPANYKMP
                     YGKDPFAPSMAKTVSGGPVAPELLAGSKSCGASGCHTEIYREWEAGAHRWSSEDVAFQ
                     GVQAALIHAEGAPAARYCAGCHDPVSLLSGYKNASTSIEAPGFKEGSSCVVCHAMRRV
                     DVQGNGNYVWGPPKPYLFEYANAGQAASITHFLIRAYPRQHDVDYDLTLDKDTASCGA
                     CHKQFIDKEINHVGWVQLQNQYDDWKHGKWNTDPNPTHRLRCQQCHMYYQEAPSVALA
                     DPYDLKSGLGLKHRNHWFAAANQWMPEVIHSPDARAQEERAIEWLQGKKYIPEIADV"
BASE COUNT          461 a          648 c          606 g          474 t
ORIGIN      
        1 cagacatcgg cgatttcagg gatatatttc ttcccttgca gccattcgat ggcgcgctct
       61 tcctgggccc tggcatcagg cgaatgaatg acctccggca tccactgatt ggccgctgca
      121 aaccagtgat tgcgatgctt aagcccgagt cccgacttca ggtcgtaagg gtcggccagg
      181 gccacgctcg gcgcttcctg atagtacatg tggcactgct gacagcgcag gcggtgcgtc
      241 ggattgggat cggtgttcca cttgccgtgc ttccagtcgt catactggtt ctggagttgt
      301 acccagccga catggttaat ttctttgtcg ataaactgct tgtggcaggc tccgcaggag
      361 gcagtgtcct tgtcaagtgt gagatcgtaa tccacgtcat gctggcgcgg gtaagcacga
      421 atcaggaaat gcgtgataga agcagcctgg ccggcgtttg cgtactcaaa taagtaaggc
      481 tttggaggcc cccagacgta gttaccgttt ccctgcacgt ccacacgccg catggcgtga
      541 caaacaacgc acgatgagcc ttctttgaaa cctggtgctt cgatgctggt actggcgttt
      601 ttatagccgg aaagcagcga gacgggatca tggcagccgg cacagtaacg ggccgcaggt
      661 gcgccttcgg catgaatgag cgcggcctgt accccttgga acgcaacgtc ttctgaggac
      721 catcggtgcg cgccggcttc ccattcccgg tagatttcag tatggcaacc tgaggcaccg
      781 cacgatttcg aacctgcgag cagttcggga gccaccggcc cgccactcac tgtcttggcc
      841 atgctgggag caaaggggtc cttgccataa ggcattttgt aattcgcagg caggctgtag
      901 tggctgaagt tcggcgcgta ggccctgtaa attcctgcga tgacgccaac cgtcgccagc
      961 agcaccaccg cagtcaccgc gctaatcagc cacatgtggc ggcggccggg gcggaagtcc
     1021 ggaagtacag agccgccatt acctttgaga ccgttcagcc cgttgttcag tccattcgct
     1081 gcctttttgg cgcgcggcca gacgtggtaa ccgagaaatg gcagcgctgc aattccgcac
     1141 cacaaatgca cctggtccca gaaacgactg gtgtacagcc cgaacatggc ctgccacgtc
     1201 agtaccaggc ccgatgccga gacaacggcc atcgtccaga atcccgtgta tgcgcaaatc
     1261 tttcggaacg accgccgagc cttgcgcgtg gcgagccaat ggctgagttg ccagatcgta
     1321 agcgggatca cgaccgcaag ccccaggagc gtgtgcatca gcaccgagac ctgggtcggc
     1381 acgctgaagg gcagcagcca gatcgccaac cccgtaaacg ccagaaagat cagcgcgccg
     1441 ctgatcagac tgaagcggaa aattcctttt tcatgaacga aagaatgcct gatgggaacc
     1501 gggccataag cagtctgttc catactgcac tcctttcttg ctctcaaacc gcaccggtag
     1561 ttggctcaac ggcgctcatc ccactctggg agttgcgcat gtcattctca gcgtcagtga
     1621 agaatctccg aggtcccgct tgcatggcat ttggaggcgt aatctttgcc attcggcgca
     1681 cagcccggtc tcgaatggga caaagcccgc agacgaatct gcgtttgctt attcttagct
     1741 gtcgaatttg cccgctctat tctccacgac aagtcatacg ctccagcgcg tgttgctttg
     1801 aaatgctatg acccgacgat ctgcccctgc tccgcatctg gctgggacaa ataaaaaagg
     1861 gcgcagccgg gtcagccatc cgctttttga gatacgaagg atgacccgct tgcgcccgca
     1921 cttccccggt ctcaaaccgg ggccgtcaga cgtttcttct atcgatcatg caacctggtc
     1981 gctacggcta ccaaaagttg ctaattatcg cggcgatttt tactcttgcg tgacctcccc
     2041 actggccgaa tcggcactga gagatgcccc gctacagaag atgagcttca cgatgattga
     2101 cgaacgctga tcaaagcttt cccgccgcat cttcggccac tagattttcc agccgtttgc
     2161 gcaagtcctc gagttggcgc gacttgaac
//
LOCUS       DASRBV010000374         4905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_601455, whole genome shotgun sequence.
ACCESSION   DASRBV010000374 DASRBV010000000
VERSION     DASRBV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601455"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2433)
                     /gene="gyrA"
                     /locus_tag="VFA02_13715"
     CDS             complement(<1..2433)
                     /gene="gyrA"
                     /locus_tag="VFA02_13715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZU10956.1"
                     /translation="MADEQNPQLPLGDGGNGDIGPGAANILPINVEEEMRRSYLDYSM
                     SVIIGRALPDVRDGFKPVHRRILYTMHEMGLQHNKKYTKCAKVVGQAMGQYHPHGDSA
                     IYDALVRLAQPFSMRYPLVDGQGNFGSVDGDPPAAMRYTECRMMRIAGELLADIDQET
                     VDFVPNYDESTYEPSVLPTRIPNLIINGANGIAVGMATNIPPHNLTEVINAAIEMVNN
                     PHAGLAEVLKHVQGPDFPTGGFIYGRSGIAQAYKTGRGRFLMRAKCAIENLTQGRQAI
                     IVSEIPYQVNKNTLIKRIAELVNDKTIDDISDVRDESDRDGMRIVIELKRGAEPQIVL
                     NQLYKHTQMQESFSMIFLAVVNGQPRELSLPDAIRHFIDHRVDVVRRRTAFQLRKARE
                     REHILIGYQIALDHLDNVIKIIRGSSSRADARENLYQFFSGKTITVRDHALKGVTLDP
                     AKYAIDPATLITPTLTLSYRQIDAILELQLYRLTQLSIDELLKELADVRERIAEYESI
                     LASEKKLRSVIVKELEAIRKEYGDVRRTEIIDEAAELKLEDLIADEQVAVTVSHQGYL
                     KRTPISTYRQQRRGGQGRTGMKTRDEDFVSQLIVESTHAYLLCFTNTGRVYWLKVYEI
                     PDVGAAGKGKSIQSLLNLQPGETVRTILGVRNLEEEGKYIFFATRYGTVKKTPLKDFS
                     NVMARGIIAIGLDEEDELVAARITDGKQVVFLATHEGMAIRFDEDDVRSMGRPARGVR
                     GIELGKNDYVVGIAVTPKERKVENGAACACLILSVTENGYGKRTDVDEYRLQSRGGKG
                     VINVKTTVRNGKV"
     gene            2722..3894
                     /locus_tag="VFA02_13720"
     CDS             2722..3894
                     /locus_tag="VFA02_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10957.1"
                     /translation="MPPSTAHSLRKFRFNRPQQIAAVLLLILLGQCLWVARHQTLTQT
                     DYEFARCGREMWEKPSPLAGYFTTCGNIHDGTLAYRAAGLPLSIQRILAGQASSTSTW
                     EMRHEVSYVLLLIRLPFIAAGLALGACLWWVTRRLYGNTGGYIALALYCFAPPIIHAC
                     TYPNNEILAAFGLFSAVYLAIGVAHAMQGPKHKWRPRIVLLTLAFGFTAAAHISAFAL
                     ALVLAIAFMAYVAEGRRSYLPTLLIIWVLGAFFLLLASYAFRPDAFSYVFRSAAGKMW
                     ISPGQSSDLFAPLQNAGITIAAIASCGLYLIFRRSRYFGNTTPLIVSLLLLVLITTGI
                     QSETWLWALPFLLTFIGGVFADVLETQYRKLFLWITGGILLAQAALCITSLPLLWQ"
     gene            3913..>4905
                     /gene="ruvB"
                     /locus_tag="VFA02_13725"
     CDS             3913..>4905
                     /gene="ruvB"
                     /locus_tag="VFA02_13725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZU10958.1"
                     /translation="MAAKAAKDDPERLVSAARAEDESSFELKLRPRWLKEFIGQTKIK
                     EQLAIALEAAKSRGEALDHVLLFGPPGLGKTTLATIIANELDVGFQQTSGPALQIQGD
                     LTAILTNLRERQVLFLDEIHRLQPVLEEKLYTALEDYKLDIIIGQGPAARTHVMDIKP
                     FTFVAATTRPGLLSSPLRSRFGILLRLEFYTDDELRYIVERSAEVLNIPIDQDGAAEI
                     AMRSRGTPRIANRLLRRVRDFAQVRGPGMIDRPTAEQALKLLEVDAHGFDELDRRLMK
                     TIIEKYDGGPVGLGTLAATLAEEEDALEEVYEPFLIQIGFLERTPRGRVATRLAY"
BASE COUNT          970 a         1486 c         1341 g         1108 t
ORIGIN      
        1 tacctttccg ttgcgcaccg tggtcttgac gttaatgacc cccttgccgc cgcgcgattg
       61 caggcggtac tcgtctacat cggtacgctt gccgtaaccg ttctccgtca cgctgaggat
      121 aaggcaggcg caggcggcgc cgttttccac cttgcgttct ttcggagtca cggcaatgcc
      181 aaccacgtag tcgtttttgc ccagctcaat gccacgcacg ccgcgcgccg gacggcccat
      241 cgagcgcaca tcgtcctcat caaagcggat ggccatgccc tcgtgcgtcg ccagaaagac
      301 aacctgcttc ccatcggtaa tgcgcgccgc caccagctca tcttcctcat ccagaccgat
      361 ggcgatgatg ccgcgcgcca tcacgttgga aaagtctttg agcggtgtct tcttcaccgt
      421 tccgtagcgc gtggcaaaga agatgtattt gccttcttct tcaaggttgc gcacgccaag
      481 aatggtgcgc accgtctccc ccggttgcag gttgagcagg ctctggatgg acttgccttt
      541 gcccgccgcg ccgacgtcgg gaatctcata gaccttgagc cagtagacgc gtcctgtatt
      601 ggtaaagcag agcaggtacg catgtgttga ttcgacgatg agctgcgaga caaaatcttc
      661 atcacgcgtc ttcatgccgg tgcggccctg tccaccacgg cgttgctggc gataagtgga
      721 gatgggcgtc cgcttcaggt atccctggtg tgagacggtg accgccacct gctcatcggc
      781 aatcaggtct tcgagcttca gttcagccgc ttcatcaatg atctccgtgc ggcgcacatc
      841 accgtattct ttgcggatgg cttcaagctc ttttacgatg accgagcgca gcttcttttc
      901 cgaggccaga atggactcat actcagcaat gcgctcacgc acatcggcca gttccttcag
      961 cagctcatca atagagagct gcgtcaggcg atagagctgc aactcgagaa tggcgtcaat
     1021 ctggcggtag ctcagcgtca gagttggtgt aatcagcgtc gccggatcaa tcgcatactt
     1081 cgccggatcg agcgtaactc ccttgagcgc atggtctcgc accgtgatcg tcttgccgga
     1141 gaagaactga tacaggttct cgcgcgcatc ggcccgggaa cttgatccgc gaatgatctt
     1201 aataacattg tccagatggt cgagcgcaat ctggtaaccg ataagaatgt gctcacgctc
     1261 acgcgccttg cgcaactgga aagcggtgcg gcggcggacc acatctacgc ggtgatcaat
     1321 gaaatggcgg atggcatctg gcagagaaag ctcgcggggc tggccattga cgaccgccag
     1381 aaagatcatc gaaaagcttt cctgcatctg cgtgtgcttg tagagttggt tgagcacaat
     1441 ctgcggctct gccccgcgct tcagctcaat gacgatgcgc attccgtcgc ggtcgctctc
     1501 atcgcgcacg tcactgatat cgtcaatcgt cttgtcattg accagctcgg cgatacgctt
     1561 gataagcgtg ttcttgttga cctgatacgg gatctcgctg acgatgatcg cctgccggcc
     1621 ctgcgtcagg ttttcgatgg cgcatttggc acgcatcagg aagcgtccac gccccgtctt
     1681 gtacgcctgc gcgatgccgc tccgcccgta gatgaagccc ccagtcggga agtccggccc
     1741 ctgaacatgc ttcagcactt cggccagccc ggcatgtgga ttgttcacca tctcaatggc
     1801 ggcattgatg acttccgtca gattgtgcgg cgggatgttg gtcgccatgc cgacggcgat
     1861 gccgttcgcg ccattaatga tgaggttcgg aatgcgcgtg ggcaggaccg acggctcata
     1921 ggtggactcg tcgtaattgg gaacgaagtc cacagtctcc tgatcaatgt cggccagcag
     1981 ctcgccggca atgcgcatca tgcggcactc ggtgtaacgc atggccgccg gagggtctcc
     2041 atctactgat ccgaagttcc cctggccgtc caccagcgga tacctcatgc tgaagggctg
     2101 cgccagacgc accagcgcat cgtagatggc tgaatcccca tgcggatggt actggcccat
     2161 ggcctgtcct acaactttgg cgcacttcgt gtacttcttg ttatgttgca gccccatctc
     2221 gtgcatcgtg tacaggatgc ggcggtgcac cggcttgaag ccatcgcgca cgtccggaag
     2281 ggcacgcccg ataatgaccg acatggagta gtcaagatac gagcgccgca tctcctcttc
     2341 cacattgatg gggaggatgt tggctgctcc ggggccgatg tcgccgttac cgccatcgcc
     2401 aagagggagt tgtgggttct gttcgtctgc catagtagat gtcacaaaat ttcacacggc
     2461 aagcaagtcg gaagtaacgg acggaagccg gatgaaatga atctatctct atgatactaa
     2521 aatagttaca agacagcaat gcagacttag cctattggat tcaggcgttt gcgggtaagc
     2581 tgccggacag ctcactaaaa tttctttcgt tatgaaattc ttaactgaaa tttttgccgg
     2641 aacttttagt tagctccttt tggttgcggg aagttttcac atctgccgtc cggtacactg
     2701 aacccagcac tttacgacac gatgccgcct tcgactgctc acagcctgcg caaattccgc
     2761 ttcaaccgcc ctcaacagat tgccgcagtg ctgctgctta tccttttggg acagtgcctt
     2821 tgggtggcgc ggcaccagac cctgacccag accgattacg aattcgcacg ctgcggccgc
     2881 gagatgtggg aaaaaccgtc tccccttgcc gggtatttca cgacctgcgg caatatccat
     2941 gatggaacgc tcgcctaccg tgccgcaggc ctgcccctaa gcatccagcg catcctcgcc
     3001 ggacaggctt cttctacctc gacctgggag atgcgccatg aagtcagcta tgtcctgctc
     3061 ctcatccggc tgccgtttat cgccgctgga ctggcgctcg gcgcatgcct ctggtgggtc
     3121 acacgcagac tctatggcaa taccggcgga tacatcgcgc tcgcgctcta ctgcttcgca
     3181 ccgcccatca ttcatgcctg tacatatccg aacaatgaga ttctggccgc gtttggactc
     3241 ttttctgccg tctatctggc cattggcgtg gcccatgcca tgcaggggcc taaacacaag
     3301 tggcgtccgc gcattgtgct gctgacgctt gcctttggat ttaccgcagc agcacatatc
     3361 tcggcctttg cgctggcact ggtgctggca attgcgttta tggcctatgt tgccgaagga
     3421 cgcaggtcgt atctgccgac cctgctcatt atctgggtgc tcggggcctt tttcctgctg
     3481 ttggcttctt acgccttccg tcccgatgcc ttcagctatg tcttccgcag tgccgccggc
     3541 aaaatgtgga tatctccagg gcagtccagc gaccttttcg cgcctcttca aaacgccgga
     3601 atcaccattg ctgccattgc ctcctgcgga ctctatctca tcttccggcg ttcgcgctat
     3661 ttcggaaaca ccactccgct gattgtctct ttgctgctgc tggtgctcat caccaccggc
     3721 atacagtcag aaacctggct ctgggcgctt ccgttcctgc tcaccttcat aggcggcgtc
     3781 ttcgccgatg tgctggaaac ccaataccga aagctctttc tctggattac cggtggaatt
     3841 ctcctcgcgc aggccgcatt gtgtatcacc agcttgccgc tgctatggca gtaactgtta
     3901 cactccggat aaatggcagc aaaagcagca aaagatgatc cggaacggct ggtaagcgcc
     3961 gcacgtgctg aggacgaaag ctcctttgaa ctgaagttgc gcccgcgctg gctgaaggaa
     4021 tttatcggac agacaaaaat caaagaacag ctcgccatcg cgctggaagc ggccaaaagc
     4081 cggggtgagg cgctggacca cgtgctgctt ttcggccccc cgggactggg caagaccaca
     4141 ctggccacca tcatcgccaa tgagctggac gttgggttcc agcagacctc cggcccggca
     4201 ctccagattc agggcgacct cacggccatt ctgaccaacc tgcgcgagcg gcaggtgctt
     4261 tttctggatg aaatccaccg cctgcagccg gtgcttgagg aaaagctcta cacggcactt
     4321 gaggactaca aactcgacat catcattgga caaggaccgg cggcccgtac ccatgtcatg
     4381 gacatcaagc cgttcacctt cgttgcggcc accacgcggc ccggcctgct ttcatcacca
     4441 ctgcgctcac gcttcggaat tctgctgcgc ctcgagttct atacagacga tgagctgcgc
     4501 tatatcgtag agcgctctgc ggaagtgctg aacatcccga ttgatcagga cggcgcagcc
     4561 gaaattgcga tgcgttcgcg cggcactccg cgcattgcca accgcctgct gcggcgcgtg
     4621 cgcgacttcg cccaggtgcg cggcccaggc atgatagacc gtcccacagc agaacaggca
     4681 ctgaagctcc tcgaagttga tgcgcatggt tttgacgaac tcgaccgccg cctgatgaag
     4741 accatcatcg aaaaatacga cggcggcccg gtcggactgg gcacgttggc agccactctg
     4801 gccgaagaag aagatgcact cgaagaggtt tacgagccgt tcctgatcca gattggtttt
     4861 ctggaacgca cgccgcgcgg acgggtggcc actcgcctcg cctat
//
LOCUS       DASRBV010000375         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_708190, whole genome shotgun sequence.
ACCESSION   DASRBV010000375 DASRBV010000000
VERSION     DASRBV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708190"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..812
                     /locus_tag="VFA02_13730"
     CDS             480..812
                     /locus_tag="VFA02_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU10959.1"
                     /translation="MTKKPDLLPGTLDMLILKTLTRGPMHGYGIAAAIKRVSDDVLTV
                     EEGSLYPALQRLLLQGWVKAEWKSSETNRRARYYTLTSAGKEQMEKETGRLAVMVEAI
                     WRVMTQEG"
     gene            complement(1055..>2188)
                     /gene="murA"
                     /locus_tag="VFA02_13735"
     CDS             complement(1055..>2188)
                     /gene="murA"
                     /locus_tag="VFA02_13735"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZU10960.1"
                     /translation="LGYGRAQHRTTISCRKLSDPVAKYEIVKTMRASALVLGPLVART
                     GLARVAMPGGCAIGGRPIDLHIKGLETMGAKISYEHGYIEARAERLKGANIVFDKITV
                     TGTEDLLMAAVLADGETVMENCAREPEVTDLAALLTAMGAKIEGAGTSTIRVQGVEKL
                     HGARHRINPDRIEAGTFLVAGAITGGDLCVSNCNPAHLGAVIRKLEEAGARVEILGKD
                     QIRVRSEGNLKAADVSTEEYPGFPTDMQAQYMALATQAEGTSIITENIFENRFMHVQE
                     LVRMGANIKVDGRTATVRGKSPLSAAAVMCSDLRASASLVLAALVADGESILDRVYHM
                     DRGYEHIEEKLRGVGAQIRRMGNIFADKREGLAIQTEPTPVNS"
BASE COUNT          442 a          574 c          710 g          462 t
ORIGIN      
        1 ttggacttac cgcatccatt ggggccgacg acgacggcaa tgccctggcc aggcagggcc
       61 acttcggtgc ggtcgcaaaa ggacttaaag cccaggatct ggattttttt cagcttcagc
      121 atgaagatgt actccttcta acctacgggc cggagagtag ctgtcagaga aaactgtagc
      181 gtccggaagg ggcagaatca acacctgtac cccaacatct tgtggataag ccgtcccaaa
      241 ctccacaaat gggagctgtc ccacagaaat tgtctcaaaa cagggctttc gcacgtagtt
      301 tttggacgat ggaacatgct gcgacggcag tcgccggaga aactaccaga tacttcgagg
      361 gtgccgcttt tttcgagtaa aaacaagcaa aaaacgcgtg aaaaggtgcc ggttttggtg
      421 cggattttcg cgtattttgc cctcgacatt ctatccatag tcaatctatg gtacttggca
      481 tgacgaaaaa acctgatttg cttcctggaa ctttagacat gctcatcctg aagactctga
      541 cgcggggacc gatgcatggg tacggaattg ctgcggcgat caagcgcgtt tccgacgacg
      601 tgctgactgt ggaggaagga tcgttgtatc cggcgttaca acggctgctt ctgcaggggt
      661 gggtgaaggc ggagtggaag agctcggaaa cgaaccggag ggcgcgatat tacacgctga
      721 cttcggcggg caaggaacaa atggagaaag agacgggaag gcttgccgtg atggtggagg
      781 cgatctggag agtgatgacg caggaaggat agccaggcca gcgcgtctgc ggcgctgtca
      841 gtaaaagggc ggtcagcgga aaggcgacca gctaagaaac ggtcagcaag ttggctggga
      901 tgcgaatggc ctgcgcccgc gccacctcgg gtgcgctagg cttcgagtcg cggatgccct
      961 gcagagcggc actgttccac tgcaagacaa acgcggtatc ctgcggagct ctcacacaag
     1021 ccttcggctt gtgcagcggc agatgagatt ccggttaaga gttgactggt gtgggttctg
     1081 tctggatggc aaggccctcg cgtttgtcgg cgaagatatt gcccatgcgg cgtatctgcg
     1141 cacccactcc tctgagcttt tcttcaatgt gttcgtagcc gcggtccatg tgatagacgc
     1201 ggtcgaggat agactcgcca tcggcgacca gcgcggccag gaccagagat gctgaagcgc
     1261 gcaggtccga gcacatcacg gccgcggctg agagcggaga cttgccgcgc acggtggcgg
     1321 tgcgtccatc tactttgatg tttgcgccca tgcggaccag ctcctgcaca tgcatgaagc
     1381 ggttctcgaa aatgttctcg gtaatgatgg aagtgccttc ggcctgcgtg gccagggcca
     1441 tgtactgcgc ctgcatgtca gtaggaaagc cgggatattc ttcagtggat acgtcggcgg
     1501 ccttcaggtt gccttcgctg cggacacgga tctggtcttt gccgaggatt tcgacgcggg
     1561 ccccggcttc ttccagcttg cggatgaccg cccccagatg cgcagggttg cagttggaga
     1621 cgcacaggtc gcctccggta atggccccgg cgacgaggaa ggtcccggct tcaatgcggt
     1681 cgggattgat gcggtgtcgg gctccgtgca gcttttccac gccttgcacg cgaatggtgg
     1741 aggtgcctgc gccttcaatc ttcgcgccca tggcggtgag cagcgcggca aggtcggtga
     1801 cctccggctc gcgcgcgcag ttttccatga cagtttcgcc atcggcaagg acggcagcca
     1861 taagcaggtc ttcagttccg gtcacggtga ttttgtcaaa gacgatgttt gcgcctttca
     1921 gccgttcggc gcgggcctcg atgtatccat gttcgtagct gatttttgcg cccatggttt
     1981 ccagtccctt gatgtgaaga tcaatggggc gtccgccgat ggcgcagccg ccgggcatgg
     2041 caacgcgggc aagtccggtg cgggccacga ggggaccgag gacgagcgca gaggcacgca
     2101 tggtcttgac gatttcgtat ttggcgacgg ggtcggagag cttgcggcaa ctgatggtgg
     2161 tgcggtgctg ggcgcgtccg tagccgag
//
LOCUS       DASRBV010000376         6857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_240598, whole genome shotgun sequence.
ACCESSION   DASRBV010000376 DASRBV010000000
VERSION     DASRBV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240598"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFA02_13740"
     CDS             <1..1174
                     /locus_tag="VFA02_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10961.1"
                     /translation="ECGTAISWCFPFFCAESIYNRTRDLAWLRQLYPRLSAFLRWTLR
                     HRTDSGGFVIGKCSWETGMDTSKRFQIQQPTGGELVEFLRLVELQAATSQAGAILARF
                     ADLISDQKSIPEWQRIQKNFAAKTQQLWEGDWFHDFDTRSMKLVNVPERDPSQAAPAF
                     CGIATESQKKQLLPTLRKMYEDMLAQAQIPESSADNALNWSSFVLPFLESAWSSGDSE
                     LASKTVENICERIYASMDRRVLRTDAKAPRLGWPGTSCEIWGAHGAFGGEVYGWGAVM
                     PAHIIRNLIGFRETEAVDQIILSPGFSPLLSGTGKQYGISRLPYGRQWISLHFTFEND
                     RNLRAEIELPQDVRVLSVTEIDGQPKRVEREGGRWQFKAANFGRYRVKFAGLSRLE"
     gene            1371..4661
                     /locus_tag="VFA02_13745"
     CDS             1371..4661
                     /locus_tag="VFA02_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU10962.1"
                     /translation="MQAFAQFSSSVQGNVLDSKGAAIPKATVTLVNLDTQVTAVKLSD
                     DTGAYRFVSIAPGHYEVSASASGFTTSKVDFTLETAENREISLTLAVGQVAATVSVTA
                     QAPLLDTSDSRNQLTIDTQAIQSLPLPARNPTSLITLTPGVTGLGAAGYNNFFTENAD
                     YSANGRGNNGNQYVLDGLDINIDVNPGVLTLVPNADALSEISVQTNTYTVDFNKTSSM
                     QTVMTTKSGTDQYHGFGSIYYTYQGLNAKGEFFPAGVKIQPFHTTNMSFGIGGPVIPK
                     KQFFFFFSIEPYLALTSNGNSVLTYEDPAFVSFAQQARPNSPEVQLMAKYPPSAATTT
                     GVAATAQDAFGPQDLTNNTGCGTPSTDNIPCSLPVFDNGVFNSSSYENAKQYNIRIDK
                     YFSKDRVYGTLFRDTSTSGGPSVRPAFNTSSPSSVIALQGSETHTFSANTLNQAFFGY
                     NYLQSTYAQKGLFSVPVVNVNNLGIGWGIGFADSQYAEHNYHWRDVLTHIHGEHAISV
                     GAEGWHGDDLALFAPAYAQPNFQYNSMIDLINDKPYSEGSLSYNPVTGKPMPGQYEYA
                     QSTFGIFAQDTWRVTHRLTINYGLRYDNYGNPYPTAGTPLANFHLGSGSTMAERVANG
                     VNRQQNHVYNQDMNWIFSPRAGVAWDPTGSGKWVVRGGFGIYRDWVTLGNAENNLKGN
                     PPGFVLPTFFNNGSTAPPIFGYGTSNTYPFGFPYPAFQGTPLDAKGGIPGQQIAVGGV
                     DGNLKSPYTETWSVGVERQLTNNMVAGVRYVGQHSGDLQYGGGNSFGNAFAGDVNVYE
                     GDLLQHLTCTPVSPPNGQQATCFGTQTRLNSSFGAINYTFNGPWSNYSGLILALKGRF
                     AQRGFVTASYTRSVSKDNSENYPIEYPLDRFYGPSPWDVPNRFSLGWSYQLPSLHSGH
                     GFLGRVASGWEISSLTVLQSGQPFSVFSSAALDVAKDVNGNLVYQPDSGDFNADGDNG
                     DFPDVSSYHISTSRKSYIQGVFPRCSGTNLDGCGPFSLPQIGQEGNERLNQFRNPGFA
                     QVDATFKKITNITEKVKLDLRVDFFNLFNRVNLNGVNSDAASGSAFGTSTSTQIPRQG
                     QIGARVEF"
     gene            5085..>6857
                     /locus_tag="VFA02_13750"
     CDS             5085..>6857
                     /locus_tag="VFA02_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZU10963.1"
                     /translation="MIQANGLAGHLDETWPDVGPNSGWLGGTGESWERGPYYLDGLVP
                     LAYLLNDARLKAKAQKFLDWTLDNTSPDGMIGPKSNNDWWPRMVMAKVLTQYHEASGD
                     PRVIPALSRYFAYQSDTLSSRPLRDWGKYRWHDEALSVIWLYNRTGDAKLLDLFRLLQ
                     QQGFDWQAEFADLKYTQRTTPEAIGLAPNTPLTDRAMQTHGVNVAMAIKMSPVRWLLS
                     QDATDRRAIFHMLEELDRYHGLPNGIFSADEHLAGRNPSQGTELCTVVEAMFSLEQSL
                     AITGEAALGDRLEKIAFNALPGAFTDDMWAHQYNQQPNQITVSLQKEPWTTDGPESNL
                     YGLDPNFGCCTANFGQGWPKFTASLFMLSNDNGLAATAYAPCEVHTVIRNTPVHVQEE
                     TEYPFRETVRLTINPASPLAFPLLLRIPAWATNASVKVNGKTLDNRPQPGMFARIERT
                     WSKGDSVEIVFPMVPRASRGYHDSISLERGPLVFSYSIGEDWLKLRTRGMTADWQVYP
                     TTEWNYALAIDAENAAESISAVETRVSEAPFTRADAPVRLQVKARQLPSWMGVDNVAN
                     PVPESPVKSTEPEKQITLIPYAAAK"
BASE COUNT         1654 a         1833 c         1746 g         1624 t
ORIGIN      
        1 cgaatgcggc acggcgattt cctggtgctt tccgttcttc tgcgctgaat ccatctataa
       61 ccgcacgcgc gacctcgcct ggttgcgcca gctttatcca cgcctgagcg cttttctgcg
      121 ctggacactc aggcaccgca cggattccgg cggcttcgtc attggcaaat gcagttggga
      181 aacaggcatg gatacgtcga aacgatttca gatacagcaa ccgactggag gcgagctggt
      241 ggaatttctg cgccttgtgg aattgcaggc tgcgacatcg caggcagggg ccattcttgc
      301 gcgttttgcg gaccttattt ccgaccagaa gagcattccg gaatggcagc gcattcagaa
      361 aaacttcgct gcaaagacgc agcagctctg ggaaggcgat tggttccacg acttcgatac
      421 ccgttcaatg aaactggtga atgttccgga acgcgaccca tcgcaggctg ctccggcatt
      481 ttgcggaatc gccacagaga gccagaagaa gcagctcttg cccacgcttc gcaaaatgta
      541 cgaggacatg ctggcacagg cgcaaattcc tgaatcatca gcagacaacg ctctgaactg
      601 gtcttctttt gttctgccgt ttcttgagtc cgcgtggtct tctggagaca gcgagcttgc
      661 ttccaaaacc gtggaaaata tctgcgagcg tatttacgca agcatggacc gtcgtgtgct
      721 tcgtacagac gcgaaagctc ctcgcctcgg atggccggga acaagctgcg aaatatgggg
      781 cgcgcatggc gcctttggcg gagaggtcta cggttgggga gcagtcatgc ccgcccatat
      841 tattcgcaac ctgattggtt ttcgtgagac tgaggcagtg gaccagatca tcctcagtcc
      901 cggattcagc cctctgctct ccggtacagg caagcagtac ggcatctccc gtctgccata
      961 cggacggcag tggatcagcc tgcacttcac atttgaaaat gatcgcaatc tgcgcgctga
     1021 aatcgaattg ccgcaggatg tccgtgtcct ttctgtaacg gaaatagatg ggcagccaaa
     1081 gcgagtagaa cgcgagggag gaagatggca gttcaaggcc gcaaatttcg gcagatatag
     1141 ggtgaaattc gcaggcctct ccaggttgga ataaggcagt ccatttgtac tatccggaag
     1201 gggttggagt ggttcatgca aactgcatac gacaacagct actctcgctg aattcagggc
     1261 agagagcgca aaaaacattc ctgttccaga ttctctactg ccgatttcag gagacagtga
     1321 tcatgcgtaa attttcaggt ctgcttccat ggtgtacttt attgatttca atgcaagcgt
     1381 ttgcacaatt ctcttcgagt gtgcagggca atgttctgga ttcaaaaggc gcggcgattc
     1441 caaaggcgac agtcacactt gtcaaccttg atacacaagt gacggctgta aagttgtccg
     1501 acgatacagg cgcatatcgc tttgtgagca tcgccccggg acactatgaa gtttcagcat
     1561 cagcttccgg gttcaccact tccaaagtag acttcacgct ggaaactgcg gaaaaccgcg
     1621 agatatctct gacgcttgcc gtcgggcagg ttgctgccac tgtttccgtg acagcacagg
     1681 caccattact tgacacttcg gacagccgca atcaactgac aattgataca caggccattc
     1741 agtcgctccc gctgccggca agaaatccta cttcgttaat tactttgact cccggagtga
     1801 ccggacttgg cgcagcgggc tacaacaact tttttactga aaatgccgac tacagcgcga
     1861 atgggcgagg caacaatggc aatcagtacg ttcttgatgg gctggacatc aatattgatg
     1921 tgaatccggg cgttctgaca ctggtgccca atgccgacgc actttcggaa atcagtgtcc
     1981 agacaaatac ctacaccgta gacttcaaca agaccagctc catgcagacg gtcatgacaa
     2041 caaaatcggg aaccgaccag tatcacgggt tcggcagcat ctattacacc tatcagggat
     2101 tgaacgccaa aggtgagttc tttccagcag gtgtgaaaat tcagccattt cacacgacga
     2161 acatgtcatt cggaatcggc ggcccggtga ttccgaaaaa acaattcttt ttctttttct
     2221 cgattgagcc atacctcgca ttgacttcca acggcaacag cgtcctgact tatgaggacc
     2281 cggcctttgt ttcttttgcg cagcaggcga ggccgaattc gccggaagtg cagttgatgg
     2341 cgaaatatcc tccgagcgct gcaacaacta cgggcgtggc cgcgactgcg caggatgcat
     2401 tcggtccgca ggacctgacc aacaataccg gatgcggaac ccctagtaca gacaacattc
     2461 cctgctcttt gcctgttttt gacaatggcg tctttaattc gtcgagctac gaaaatgcaa
     2521 aacagtacaa cattcgcatt gataaatatt tcagcaaaga ccgggtttac ggaacacttt
     2581 tccgcgacac aagcacttcg ggtggcccat cggtccgtcc ggcattcaat acttcaagcc
     2641 catcctcggt gattgcgctc caggggagcg aaacacatac tttttcggcc aacacgctga
     2701 accaggcatt ctttggctat aactatctgc aatccacata tgcgcagaaa gggctgtttt
     2761 ctgttccggt cgtaaatgtc aataatctgg gaattggctg gggaatcggt tttgctgatt
     2821 cccaatatgc cgaacacaat tatcactggc gcgatgttct cactcacatt catggagaac
     2881 atgccatcag cgttggggct gaaggctggc acggcgacga tctcgcgctc tttgctccgg
     2941 cctacgctca accaaacttc cagtacaaca gcatgattga cctgatcaat gacaagccat
     3001 attcggaagg cagtctttct tataacccgg ttacgggcaa gccgatgccc gggcagtatg
     3061 agtatgccca aagcactttt ggcatttttg cgcaggacac atggcgcgtg acccaccggc
     3121 tcacgattaa ctatggcctt cgttacgata actatggcaa tccttatcca acggctggca
     3181 cgcctcttgc taattttcat cttggctccg gctcaaccat ggccgaacgt gtggcaaacg
     3241 gcgtcaaccg gcagcagaac cacgtctata accaggacat gaactggatc ttcagcccac
     3301 gcgcaggtgt agcatgggac ccgacaggaa gcggtaaatg ggtcgtacgc ggaggcttcg
     3361 gaatctatcg tgactgggtc acattgggta atgcggaaaa taatctgaaa ggaaatccgc
     3421 ctggatttgt tcttcctacg ttcttcaata acggctcgac tgcaccaccc atttttgggt
     3481 acggcacatc aaatacgtat ccctttggct tcccatatcc tgcatttcag ggcactcctc
     3541 tggatgcgaa aggcggtatt cccggccagc aaattgcagt tggtggcgta gatggcaatc
     3601 tgaaatctcc ttacaccgaa acatggtctg tgggtgtaga acgccagctc acgaacaata
     3661 tggtggcagg cgtgagatat gtcggacaac attccggtga tttgcaatat ggcggaggca
     3721 attcctttgg caatgctttc gcaggagacg tcaacgtcta tgaaggtgac ttgttgcagc
     3781 accttacctg cactcctgtc agtcctccaa acggccagca agccacctgc ttcggcacgc
     3841 agactcggct caactcaagt tttggcgcaa ttaactatac ctttaacgga ccgtggtcca
     3901 actactccgg cctgattctc gcgctgaaag gacgtttcgc gcagaggggg tttgttaccg
     3961 cttcttatac ccgttcggta tcgaaggata attcggaaaa ttatccgatt gagtatccgc
     4021 tggacagatt ctatggccca tctccgtggg acgtgccgaa ccggttctcg ctcggatgga
     4081 gctatcaatt gccatctttg catagtgggc atggtttcct gggaagagtg gcctctggat
     4141 gggaaatatc ttcgcttact gtgctgcaat ctggacagcc attcagtgtt ttctcaagcg
     4201 ccgcattgga tgtagcgaaa gatgtaaatg gcaacctggt ttatcagccg gactcaggcg
     4261 actttaacgc tgatggggat aatggagatt ttccggacgt ttcttcgtat cacatctcca
     4321 ctagccgcaa aagctacatt cagggggtct tcccgcgctg ttctggaaca aacctggatg
     4381 gctgcggtcc attctctttg ccgcaaatcg ggcaggaggg aaatgagcgg ctgaatcaat
     4441 tccgtaatcc cgggtttgct caggtagacg ccactttcaa aaagatcacc aatattaccg
     4501 agaaggtcaa actggattta cgtgtagatt tcttcaatct gttcaatcgc gtaaatctca
     4561 acggcgtcaa ttccgatgcg gccagtggat cagccttcgg aacatctacc agcacacaga
     4621 ttccccgtca gggacagatt ggggcaagag tggaatttta aagaaatcgt ccgaaatata
     4681 tgaacctgga ggcgtgcaga ctgaattttg tacgcttcca attccttgag ttttctgccg
     4741 ctctacttaa cacaactttg tatgcgcaat agaccattca taccatgtgc tgcatgtgat
     4801 gaagtcatta acatagacaa tagttcgcgg agattcgttc tatcatgtgt tttcctgggc
     4861 cttctcattc tcatttttcc cgccgccgtt ttctcgtttt tcttggagca gccggcattg
     4921 cttcaatttc aggtgatgct tttggaactg ccacccaagc tacaggtgag gcgggccgta
     4981 agagtgcggc accgataaaa aatcgcgcac cgctggcaca gaatactttt tataccttgc
     5041 cgcttgggtc catccgccca acaggatggc tgcgcgatca gcttttgatt caagctaatg
     5101 gactcgcagg acatcttgat gaaacatggc ccgatgtcgg ccccaacagc gggtggcttg
     5161 gcggaacagg agaatcctgg gagcgcggtc cttactatct tgatggactc gtgccgctgg
     5221 cttatctgct gaacgacgct cgcctgaaag caaaagcaca aaaatttctt gactggacac
     5281 tcgacaatac ttcacctgac gggatgattg ggccaaagtc caacaatgac tggtggccgc
     5341 gcatggtgat ggcgaaggtg cttacccaat accatgaagc ctctggcgac cctcgcgtca
     5401 tccctgcatt atcaagatat tttgcctatc agagtgatac cctgtcttcc cgtccgctcc
     5461 gcgactgggg aaaatatcgc tggcacgatg aggccctctc agtcatctgg ctgtacaacc
     5521 gcaccggaga tgcaaagctg cttgatctgt tccggctctt acagcagcag ggcttcgact
     5581 ggcaggctga gtttgcggac ctcaaataca cgcaacgcac tacaccggag gcaatcggtc
     5641 tggcgccgaa tactccgctc actgaccgcg caatgcagac acacggtgtc aatgtggcca
     5701 tggccatcaa gatgtcgcct gtgcgctggc tgctttcaca ggacgctacc gaccgccgtg
     5761 ccatctttca catgctggaa gaactcgacc gctatcacgg cctgccaaac ggtattttct
     5821 ccgctgatga gcatcttgcc gggcgtaatc cgtctcaggg aactgagtta tgcacggtgg
     5881 ttgaagccat gttttctctg gagcagtctc ttgccatcac aggagaagca gccctgggag
     5941 accgcctgga aaagatagcc ttcaatgctc ttcccggcgc atttactgac gacatgtggg
     6001 cgcatcaata caatcagcag cccaaccaga tcaccgtaag ccttcaaaaa gaaccctgga
     6061 ccacggacgg cccggaatcg aacctctatg ggctggatcc gaatttcggc tgctgtactg
     6121 caaattttgg ccagggatgg cccaaattta ctgcaagcct cttcatgctc tcgaatgaca
     6181 atggccttgc tgccacggcg tatgctccgt gcgaagtcca cacggtaatc cgcaacacgc
     6241 ctgttcatgt gcaggaagag acagagtatc catttcgcga aactgtgcgc ctgaccataa
     6301 atccggcatc gccactggca tttccactct tgctgcgaat tccggcctgg gcaaccaatg
     6361 cgagtgtcaa agtaaatggg aaaacactgg acaacagacc gcagcctgga atgtttgctc
     6421 gcatcgaacg cacatggagc aagggagaca gtgttgaaat agtgttcccg atggttccac
     6481 gcgcatctcg cggatatcac gactctatca gcctggagcg tggacctctg gtattttcct
     6541 acagcattgg agaggactgg ctcaaactgc gcacacgtgg catgaccgcc gactggcagg
     6601 tctatcccac aacggaatgg aactatgcgc ttgcaattga tgcggaaaac gccgcagagt
     6661 caatctcagc cgtggaaacc cgagtttcgg aagctccatt taccagggct gatgcgcctg
     6721 tcaggttaca ggtaaaggcg cgccagcttc cttcctggat gggcgtggac aatgtcgcca
     6781 acccagtacc agaaagtccg gtaaagagca ccgaaccgga aaaacagata acacttattc
     6841 cttatgctgc tgcaaaa
//
LOCUS       DASRBV010000377         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_287528, whole genome shotgun sequence.
ACCESSION   DASRBV010000377 DASRBV010000000
VERSION     DASRBV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287528"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFA02_13755"
     CDS             <1..290
                     /locus_tag="VFA02_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10964.1"
                     /translation="KALTALSLLMGIFQYQPSPFCVLDEVDAPLDETNVGRLAEMLRS
                     MATDTQFVMVTHSKRMMTAADLIYGVTMQEPGVSKVVSVRLGGQEKQRATA"
     gene            503..2797
                     /locus_tag="VFA02_13760"
     CDS             503..2797
                     /locus_tag="VFA02_13760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZU10965.1"
                     /translation="MPHPYQAGGRTLLLRISAGVLMLVLCSPAYAAHAPLSPYEKAQR
                     LHEALEGRPEQQRTRRDYERVLNAYRAVYHGDPASPKADASISAVAGLLAEQGRLFQD
                     EKSLHDAIGQYEFLRRQYPGSRFRYSALLTEGEIYLHDLDDRDSARQTFQQFLKLYPK
                     STLADEARQELSNIRKQEIAERRGKHTVTQAKSTPGPKPSAPPAKTAETNSASAAKPM
                     EAPREQSSAFVEPAHAQEPLEGPKLSEAAMNAPPPPPRKGRLPLVTGIRHWSTPVYTR
                     VAIDLQDEAQYEASRVPNPDRIFFDLHGTRLAPELIGKSVDVTDDGFLKRVRAAQFSN
                     DVTRIVLDVSDVSEYSAFLLPNPWRLIIDVHGKKPGTPPQQQTASTQPQPSPQPRQET
                     SPPAPQTATQKKPVAPQITQATPAQEQSASASTPNNTGTQSSSLKDIAQLDRQPNRVK
                     ATRHPTTAPVVQTVAGTKPEEQPNELEALNNIPPPVPAPVQKKSSKPTRVVDADAPPV
                     HEATPTATGERSMVRALGLKIGRIVVDAGHGGHDSGTLGPGGIEEKDVVLDVALRLGK
                     LLRQRLGADVIYTRDDDTFIPLETRTAIANKAQADLFISIHANSSSDPSARGVETYYL
                     NFTTSADALEVAARENAVSDQSIHQLSDLVKKIALQDKISESREFASDVQQSLYSGLE
                     NGNPGLKDRGVKKAPFVVLIGANMPSILAEISFLTNPDDARELRDPDYRQRIAESLYR
                     GVARYVGSLSGVRIAQNSGRSSGK"
     gene            2997..>4402
                     /gene="cysS"
                     /locus_tag="VFA02_13765"
     CDS             2997..>4402
                     /gene="cysS"
                     /locus_tag="VFA02_13765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZU10966.1"
                     /translation="MTIRLFNTLTSHIEELRPLDGETLRIYACGPTVYDYGHIGNFRT
                     FLHVDVLRRVLELNGVKVRHVMNITDVDDKIIRNATAAGVPIGEYTRRYEKAFFEDLD
                     TLSVEHPEIIARATEHIADMVSLIQRLAAQNLAYKTDDGSWYFRIAAFPEYGKLSKKD
                     FAGITDGARVDVDEYDKDSARDFALWKAPKPGEFFWETELGPGRPGWHIECSAMAMRY
                     LGESFDLHAGGEDLMFPHHENEIAQSESATHKPFAHHWFHVRFLLVEGRKMSKSEGNF
                     YTLRDLLLKGHKASAIRFLLISVPYRQQLNFTFEGLAAETGAVERLRTFHQRLHSVAP
                     AQQTNQHLAEETARASQAFRSALAHDLNTAEARAAVFELVRHANAAMDARTLGAENIA
                     QIQGLLADFDKIFAVLEDRDAEWTRFALEWAEREGKLDQVAPEVRAQLSLTDAQIEAL
                     IEERNQARRVRNFARADQ"
BASE COUNT          967 a         1425 c         1160 g          850 t
ORIGIN      
        1 aaaaggcact caccgcttta tcacttctga tgggcatctt ccagtaccag cccagcccct
       61 tctgtgtgct cgacgaggtg gatgctccgc tcgacgaaac gaacgtgggc cgtctggcag
      121 agatgctacg cagcatggcc actgacacgc agtttgtcat ggtcacgcac tccaagcgca
      181 tgatgaccgc cgctgatctc atctatggag tgacaatgca ggagcctggc gtctctaaag
      241 tggtgagcgt gcggcttggc ggacaggaaa agcagcgggc gaccgcgtga gagattcgtt
      301 attttccagc caatgcgcaa agcgcatcag tctgctgcgt tccatgatga agccccaaac
      361 tcatggaaac gcagcagaaa catccttcca ttttggtttc catatgagtt tccctcccgt
      421 ttttccggct tttctgaatt gcccgcattt cctgcgcacc ctataatcaa gatgtctctg
      481 gcgtgtgcct ctgaatctgt ttatgccgca tccttaccaa gctggaggac gcacgctttt
      541 gttgcgcatc agcgccggtg tgctgatgct tgtcttgtgc tcccccgcat atgcggcgca
      601 cgcgcccctt tctccttatg aaaaggccca gcggttgcat gaagccctcg aaggccgtcc
      661 cgaacagcag cgcacacgtc gtgattacga gcgggtcctg aatgcatatc gcgctgttta
      721 tcacggagac ccggcatcgc caaaggccga cgccagcatt tctgccgtcg ccggtcttct
      781 ggctgaacag ggccgcctct tccaggatga aaagtctctg catgacgcca ttgggcagta
      841 tgaatttctg cgcagacaat atccgggcag ccgttttcgc tacagcgccc tgctgacaga
      901 aggtgaaatc taccttcacg atctggatga tcgcgacagt gccagacaaa cctttcaaca
      961 atttctgaag ctctatccca aaagcacgct cgctgacgag gcccgccagg aactgagcaa
     1021 catccgcaaa caggaaattg cagaaaggcg cggaaagcat accgtcaccc aggcaaaaag
     1081 cacaccaggg ccaaagccat ccgcaccgcc tgcaaaaacg gctgaaacaa actctgcttc
     1141 tgccgcaaag cccatggaag ctcccaggga gcaatcgtcc gcgttcgtgg aacctgctca
     1201 cgcgcaggaa ccgctcgaag gaccaaagct gagtgaagca gccatgaatg ctcctccgcc
     1261 cccgccgcgt aagggcaggc tgcctttggt gactggcatt cgccactggt ctactccggt
     1321 ctacacccgc gtcgccatag atttgcagga cgaagcacag tacgaggcct cgcgtgtccc
     1381 caatcctgac cgcatcttct ttgacctgca tgggaccagg ctcgctccgg aactcatcgg
     1441 caagtctgtg gatgtgaccg acgatggttt cctgaagcgc gtgcgtgcgg cgcagttttc
     1501 aaacgacgtc acccgtatcg tgctcgacgt cagcgatgtg agcgagtact ccgcgtttct
     1561 gcttccgaat ccgtggcgtc tgattattga tgtgcatggc aaaaagccgg gaaccccgcc
     1621 acagcagcag accgcctcca cccagcctca accatccccg caaccgcggc aggagaccag
     1681 ccctccggca ccgcagacgg ctacccagaa gaagcccgtc gcgccgcaga taacccaggc
     1741 gacgcctgca caagagcaat ctgccagtgc gtcgactccc aacaataccg gtacacaaag
     1801 ttcatcgttg aaagacattg cgcagcttga ccgccagccc aaccgggtca aggcgacacg
     1861 ccatccgacc acggccccgg ttgttcaaac tgtcgccggc acaaagccgg aagagcagcc
     1921 aaacgagttg gaggcgctca acaacatacc gccgcccgtg cctgctccgg tgcagaagaa
     1981 aagcagcaaa cccacacgcg tggtggatgc agatgctcct cctgtccatg aagccactcc
     2041 taccgctacc ggagagcgtt cgatggtgcg tgctctcggc ctcaaaatcg gccgcatcgt
     2101 tgtagacgcc ggacatggag gacatgattc cggcacgctg ggcccgggtg gaatcgaaga
     2161 gaaagacgtt gtgctcgatg tagcgcttcg cctgggcaag ctgctcaggc agcgcctcgg
     2221 cgcagatgtc atttacaccc gcgacgatga caccttcatc ccgcttgaaa cacgcaccgc
     2281 gattgccaat aaggcgcagg ccgatctttt tatctccatc catgccaact ccagctctga
     2341 cccgtcagct cgtggagtgg aaacgtacta cctgaacttc accacctcgg ccgacgcgct
     2401 cgaagtggca gcgcgcgaaa acgctgtctc cgaccagtcc atccaccagc tttctgacct
     2461 ggtcaaaaag atcgccctgc aggacaaaat cagtgagtcg cgcgagttcg cctccgacgt
     2521 gcagcaaagc ctctattcag gacttgaaaa tggcaacccg ggactgaaag accgcggcgt
     2581 aaagaaggcg ccgttcgtcg tgctcattgg cgccaacatg ccgtctattc tggccgaaat
     2641 ttcattcctc accaacccgg acgacgcccg cgaactccgc gaccctgatt atcgccagcg
     2701 catcgccgaa tcgctctatc gcggagtcgc ccgctatgtc ggcagtctca gcggagtgcg
     2761 catcgcccag aacagcggcc gctcctcggg caagtaggcc tgttccaatg gatgtctctt
     2821 cctgtgtttt caagacggtt gagtgtacaa ccagattgcg gcctgtgctt cccataaatt
     2881 tttaattttg cgcacacaac gtccgggctt gttcaagctg ctgccgccag ccttccttcg
     2941 cgccgcctgg gcggtgaatc caactgatac aatcgcaagt gaaccgtagc gcacgaatga
     3001 ccatccgtct tttcaatacc ctcaccagtc atatcgaaga actcaggccg ctggacggcg
     3061 aaaccctgcg catctatgcc tgcgggccta cggtctatga ctacggacac atcggcaact
     3121 tccgcacttt tctgcatgtg gacgtgctgc gccgtgtgct cgaactgaat ggggtcaaag
     3181 tcagacacgt catgaatatc accgatgtgg acgacaagat catccgcaat gccacggccg
     3241 ccggtgtgcc catcggcgaa tatacacgcc gctacgaaaa ggcattcttt gaggacctgg
     3301 acacgctttc cgtcgagcac ccggaaatca tcgcgcgcgc caccgagcac atagcggaca
     3361 tggtctcgct catccagcgc ctggccgcgc aaaatctggc ctacaaaacg gacgatggct
     3421 catggtactt ccgcatcgcc gcattcccgg aatacggcaa gctctcaaag aaggacttcg
     3481 ccggcatcac cgacggcgcg cgtgtagacg tggatgagta cgacaaggat tcggcccgcg
     3541 acttcgccct ctggaaggcc ccgaaacccg gcgaattctt ctgggaaacc gaactcggtc
     3601 ccggacgccc cggctggcac atcgaatgct ctgccatggc gatgcgctac ctcggtgaat
     3661 ctttcgacct gcacgcaggc ggcgaagacc tgatgttccc gcaccacgaa aatgagattg
     3721 cgcagtccga atcggccacg cacaaaccct tcgcgcacca ctggtttcac gtgcgttttc
     3781 tgctggtcga aggccgcaag atgtccaagt ccgagggcaa tttctatacg ctccgcgacc
     3841 ttctgctcaa gggccataag gcctcggcca tccgctttct gctgatttca gtcccctatc
     3901 gccaacagct caatttcacc ttcgagggac tggctgccga gaccggggcc gtcgagcgcc
     3961 tgcgcacctt ccaccaacgc ctgcactccg tcgcaccggc gcagcagaca aaccagcatc
     4021 ttgccgaaga aactgcacgc gcctcgcagg cttttcgctc cgctctcgcc catgacctga
     4081 acacagccga ggcccgcgcg gccgtctttg aacttgtccg ccatgccaat gccgccatgg
     4141 acgcccgcac cctgggggca gagaacattg cgcagataca agggcttctc gccgactttg
     4201 acaaaatttt tgctgtcctg gaagaccgcg atgccgagtg gacccgcttt gccctcgagt
     4261 gggccgaacg cgagggcaaa ctcgaccagg tcgcgcctga agtccgggcg cagctctcac
     4321 tgaccgatgc gcagattgag gccctgatcg aagaacgcaa tcaggcgcgc cgcgtccgca
     4381 acttcgcccg tgccgaccag at
//
LOCUS       DASRBV010000378         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_340592, whole genome shotgun sequence.
ACCESSION   DASRBV010000378 DASRBV010000000
VERSION     DASRBV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340592"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFA02_13770"
     CDS             complement(<1..324)
                     /locus_tag="VFA02_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HZU10967.1"
                     /translation="MARTKANTQEIPKIEAVVPTAAIPGGEVELLGESLGPVAQRQPI
                     VMVGQLAASVLLSRSRRIKLKVPDEAETGPVRVLQNGAQSNDARVLVAGMIATDLHIV
                     ANPAVD"
     gene            complement(356..835)
                     /locus_tag="VFA02_13775"
     CDS             complement(356..835)
                     /locus_tag="VFA02_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZU10968.1"
                     /translation="MTKNKTFWAWVTATFFGAGFGKPGPGTWGSVAAALLWFLVARIA
                     HLSQAGVAALSVTGALAALATGIPAGTIVARESGREDPGHVVLDEVCGQWIALAACPP
                     EWGYALLSLILFRVFDITKPWPARRLERLHGGAGIMLDDVAAGIYALVVFVVIHHWW"
     gene            complement(822..1052)
                     /gene="yacG"
                     /locus_tag="VFA02_13780"
     CDS             complement(822..1052)
                     /gene="yacG"
                     /locus_tag="VFA02_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZU10969.1"
                     /translation="MSSQSSIKNKVLRCPTCGTIVTPSDENFPFCSERCQLIDLGKWA
                     SGAYRISSPVLDPEVLEDIESAPRPRKDDDEE"
     gene            complement(1335..>2280)
                     /gene="rimO"
                     /locus_tag="VFA02_13785"
     CDS             complement(1335..>2280)
                     /gene="rimO"
                     /locus_tag="VFA02_13785"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZU10970.1"
                     /translation="DHTTPRLLATRGPSAYIKIAEGCDHPCSFCVIPNLRGKFRSRHF
                     ESVVAEAERLVAQGVEEITLIGQDTTCYGEDLGLKDGLALLLDKLAGIEGLRWLRFLY
                     AYPNKVTAPLLDAIARHENICKYLDVPLQHASGPVLKTMKRGANAEIFLKMIEKVRAA
                     VPGIALRTSFIVGFPGETDADFEALQDFVREAKFDWLGVFSYSDEEGSGAFSLADKVP
                     AKVIEQRRRALMRLQKGISKKAKQQWVGRTMTVLSEGESEETPLLWQGRSQFHAPEID
                     GTVYINDFGPYEVIEAGKFYQCEITEAHDYDVVARVVE"
BASE COUNT          513 a          751 c          538 g          478 t
ORIGIN      
        1 gtctacggcc ggattcgcca caatatgcaa atctgtagca atcatcccgg ccaccagcac
       61 acgggcatcg ttgctctggg cgccattctg cagcacgcgc actggcccag tctcagcttc
      121 atccgggacc ttgagcttga tccggcggct gcggcttaaa agaacagaag cagccagttg
      181 gcccaccatc actatcggct gccgctgcgc caccggcccc agactctccc ctagcagctc
      241 tacctcgccc cccggaatgg ctgccgttgg cacaactgct tctattttcg gtatttcctg
      301 cgtatttgct tttgtcctag ccatgtcctg aaaccagcgg cgcattgccc agtccctacc
      361 accagtgatg tattacaaca aataccacca gtgcatagat tccggcagcc acatcatcga
      421 gcataatacc tgcgccgcca tgcaggcgtt ccagccttcg cgctggccac ggttttgtga
      481 tgtcaaaaac ccgaaaaagt atcaaagaca gcagcgcata cccccactct ggcgggcaag
      541 cagccagcgc tatccattgt ccgcagacct catccaacac cacatgcccc ggatcttcgc
      601 gcccgctctc ccgcgctacg atggtccccg cgggaatgcc ggtcgccagc gccgccagcg
      661 cgcctgtgac agaaagtgct gcaactcctg cttgtgaaag atgcgcaatt cgcgccacaa
      721 gaaaccagag cagcgctgca gccaccgaac cccaggtccc cggtccaggc ttaccaaatc
      781 ccgccccaaa aaaagtcgcc gtcacccacg cccagaacgt cttattcttc gtcatcgtcc
      841 ttccgtggcc gcggggccga ttcaatgtct tccagcactt cagggtcgag caccggcgag
      901 gaaatccgat aggccccact cgcccactta cccagatcaa tcaactggca gcgctcgctg
      961 caaaaaggaa agttctcatc actgggagtc acaattgttc cacaggtggg gcagcggagg
     1021 accttgttct taattgaaga ctgactgctc ataactgatt tgatgaaaac gccttgtatc
     1081 tcgtctctga tgagagtcta gcaagcagaa tgagttttta tcccacctca cctcaaagga
     1141 attagggctt atgtgcagca gctgaatcac tatgcatttt ccgtatattt acggtccgtc
     1201 attctgacga ccgaagggag gaagaatctt agcgatgtaa gcttttagcc aatcctgcta
     1261 agatttccgc tccgcccgga acgacgttcc ctcttttcgc agatcgaagt gacacgaaag
     1321 atgcactcac ccactcactc cactacccgc gccaccacgt cgtaatcatg cgcttcggtg
     1381 atctcgcact ggtagaactt ccctgcctca atcacctcat atggtccaaa atcattgatg
     1441 tagaccgtgc cgtcaatctc cggcgcgtga aactgcgatc ttccctgcca tagcagcgga
     1501 gtctcctctg actcgccttc cgacagaaca gtcatcgttc gtcctaccca ttgctgcttc
     1561 gcctttttac tgatgccctt ctgcaaacgc attagggcac gcctgcgctg ctcaatgacc
     1621 ttcgccggaa ctttatcagc tagtgaaaag gctccggaac cttcctcgtc cgaatagctg
     1681 aacaccccca accagtcgaa ctttgcttcg cgcacaaaat cctgcaacgc ctcaaagtcc
     1741 gcatcggtct cacccggaaa cccgacaata aatgaagtcc gcagcgcaat cccgggaaca
     1801 gccgcgcgca ccttctcaat catcttgagg aaaatctcgg cattcgcgcc tcgcttcatg
     1861 gtcttgagca caggcccgga agcgtgctga agcggaacgt cgagatactt gcagatgttc
     1921 tcgtgcctgg cgatggcatc aagcagcggc gctgtcacct tgttcggata cgcatagaga
     1981 aagcgcagcc agcgcaagcc ctcaatgcca gccagcttgt caagcagcag cgccaggccg
     2041 tccttcaggc cgaggtcctc gccgtagcag gtcgtgtcct gcccaatcaa cgtgatctcc
     2101 tcaacaccct gcgcgaccag cctttccgcc tcggcaacca ccgactcaaa atgccgcgag
     2161 cggaacttgc cccgcaggtt cggaatcacg cagaagctgc atgggtggtc gcatccctcg
     2221 gcaatcttga tatatgcaga aggcccgcgc gtcgccagta gtcgcggagt ggtgtggtca
//
LOCUS       DASRBV010000379        21583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_184648, whole genome shotgun sequence.
ACCESSION   DASRBV010000379 DASRBV010000000
VERSION     DASRBV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 21583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21583
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184648"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..437)
                     /locus_tag="VFA02_13790"
     CDS             complement(177..437)
                     /locus_tag="VFA02_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10971.1"
                     /translation="MPKFQMYFDTVRGETRYNVDIDEDETLSEVLEEILFDLRERGDV
                     LAGEGEPQVIWNGQSLDFSIPLYQQGVRPNDVLRISTIATNG"
     gene            complement(440..1159)
                     /locus_tag="VFA02_13795"
     CDS             complement(440..1159)
                     /locus_tag="VFA02_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10972.1"
                     /translation="MTARSGTVAQVAGLLAIAFTVLLIAGIAGNVPLIVVMLLVGIAT
                     PFVASLCDVQFGQWWMLPAVLTATFCVSLAVLTAARHTGTVTWTLPLVLFAVISLAAL
                     LSRMTKRRCALCHRRLGPHAITFGCPRCGLEVCDEFCWNFEHRRCRMCEENRVPILPA
                     QSQWWDRQLGPRVQHGRCQLCMASPEQSDLRSCGHCHRPQCRDCWDQTNGECSRCGWT
                     MPNLPKSLETVAVHLPVHMER"
     gene            complement(1169..2254)
                     /locus_tag="VFA02_13800"
     CDS             complement(1169..2254)
                     /locus_tag="VFA02_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZU10973.1"
                     /translation="MGLSKLKRTSYQSCSIETLSVALVGLGALGSEIARLLGVLGIGT
                     VALVDNDKVESVNSLQSIFFRNSDALGCYKAEVIANHGLTYFPRTRWIPLVAEIADTG
                     FKGLKNCALMISATDSALSRVETAYVSRKLRIPMLDAGLSGPAYWRGRVAWFPAETNA
                     ACYCCQLSENRRAEILSLTCSPVLSCRWAEENRDMPSTPTMASVVAGMAVDLAFRHGL
                     FAKEESSFAWEIDLGNPPGLVRHVLSHSSTCPFHDLSSSNTLVELAYNEPFQNSLTSL
                     DADAFELDWPIAIHATCLRCHHRWQPLRRMAWVRRYGRCQQCRESARIRYEAIDKITR
                     NSGEAQFSPADLSYAEDHLYTPVYHTR"
     gene            2471..3367
                     /locus_tag="VFA02_13805"
     CDS             2471..3367
                     /locus_tag="VFA02_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZU10974.1"
                     /translation="MAWGVNAMIGAIVETPSAAWMADAILLGLVAVLLIPFLLAQLDR
                     YSGRSFRWGTLWIGVLIAILSALIASLLLWFLRSNIAVESPFLYRLAGWSLAASILGL
                     GIGSRWLTSNKLRILHTYTGGLAGGLLGGLLFVGFGGHQPELCQAAGLMLTGAGIGFG
                     AGLAPLLIRDGAVQFISSADARAQSKYGRANKFWELQHEETYVVGNLATSQSETRFQP
                     GAEIFIPDASMAARHAVLFSKEGRFYIARHPDAGGPAGIAKYVLRVRGKTVTTSQELT
                     EADDILIGRTALRFTTRKQGEP"
     gene            3364..4803
                     /locus_tag="VFA02_13810"
     CDS             3364..4803
                     /locus_tag="VFA02_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZU10975.1"
                     /translation="MRSKLLICCCLVLAAASAAAATPASLRQTGPATLHDCSPVTLVP
                     CFTVGVGPVDQNGAPVGVLLPPQDRLTQAVSIQTSNGAIAPFYVKAAAGGDAVARPNV
                     VLIEIDISGSMNETIAPGTSRFAAAKQAIQNYLAMMQDGVDEVAIVPFESHHVVPTIQ
                     AAVFTSKRDQAVAQLNALPEPGPKNNTALYQAVFTGVDTLNAEIARLEKKGTPRGNVT
                     PQLIVMTDGKNEVFHGDDPVLLDGPLGLEQASAKVRDAGYEVIGIGFGNASDIDAEAL
                     KKISTRYFLASDPAQLAQVFRSSTPVHPSELEIAFLAPVTDRHSLAARNANFVVSLHL
                     LDGTVLTSPAISYVPPALGFPLYETHLDREALQALAAVEPPAISGWTIVLRGILVFCG
                     LGVILLLLWFWVPRIVWRGGIPAAGTRRRWSKESTVQASGVQVRDTPNGFDTEAAASS
                     PQRTAAQVTQVRLRTDLTNAAPQSGNKGQ"
     gene            4903..5334
                     /locus_tag="VFA02_13815"
     CDS             4903..5334
                     /locus_tag="VFA02_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10976.1"
                     /translation="MMLPMTPERIGAEAASENEEPVKIEDLNDAFRKFQPKLKFTGHA
                     GEDEAEFKAEFRFRSVKDFDPENLQKKQEIRKEDGEVEYLRNDIADLKTNIDLLYRLK
                     DRWKLPTVRRAWANEAQRKEIIAALGKLRSELERVANGGKQ"
     gene            5334..6692
                     /locus_tag="VFA02_13820"
     CDS             5334..6692
                     /locus_tag="VFA02_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10977.1"
                     /translation="MADTQTITRTKTTEELIADSPARELLQDIFADLHPALTDEKREL
                     ASIFTPQSAKEFLSQLTNFDQLLDEKDSYNDSVQKLQQQIEKAESVRDELLRQVFEQI
                     RPIERSYRQLQLFFENSKVMDGKVRKPVEVYVLNADPTAMKDIFSMSVAAVENFVKSR
                     NDNFNFRDDICNLVVPGFYPQSIREKFEQIANQWGMLLISDLDDEKSFRNVERNFLPG
                     GKYEFLKRPEDQAAADVCLMGYLQLRAPYWFEKDQSNDGLYAPPSVAFAGAVARADDA
                     QGLAQGPVGSRFGRVHGVEKARIEPLIGEMEHLSMERQLIPVIRDADNHLCFFGCRTQ
                     ADDPYGVLKFFTSYRILRYLERCCRHYLLQVAGQVLTRDFMDQQIEAPLKRLLDEQVE
                     QGTILSYDLFVDKDSNKRMQGICDITLSVMPTGPAETFVLKIDVPEFSKPTNPEAKQA
                     NA"
     gene            6719..7156
                     /locus_tag="VFA02_13825"
     CDS             6719..7156
                     /locus_tag="VFA02_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10978.1"
                     /translation="MSLGPHKKSIEPGDSARRAEQGPHPAPFRRIDGGTAARDAEQRS
                     YDTSIGRTVHGFTLSARVGKPFKFENGVPAAAGEAMPAESPASAPEIQIKKTNQTQNE
                     KPMNVAAPEKPHVADSSAISFPGSFLRALFRRRFPSASPENDT"
     gene            7186..7665
                     /locus_tag="VFA02_13830"
     CDS             7186..7665
                     /locus_tag="VFA02_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10979.1"
                     /translation="MAASPYRTTVTIDGTKFDAISTSVRFSTEKDRSGVPQMGSLATS
                     VRVWADFHDDQNIPFSTVQKLFNLANVVTRDKIRSIKIEYWKDDSKQDALVSYSFNGW
                     IRRFETSNPLDFVRATSTSSDEDNFVSGIYSNTNHLLVLDIEPALNQQNFQNVQVSN"
     gene            7682..8131
                     /locus_tag="VFA02_13835"
     CDS             7682..8131
                     /locus_tag="VFA02_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10980.1"
                     /translation="MAASPNRSTLTIDGNKFDCVSVSVALQTEKDRAGMPQMGSLSTS
                     IKAYVDFHDDQNLPFSTLKKLFDLSNVVTRDKIRDVKIEFWKDDAKQDALVSYNFKGW
                     ISSFHTLNPYQNGQPSDNDHIGVNHMLVLELEPVLNQQNFKDVTISN"
     gene            8251..8523
                     /locus_tag="VFA02_13840"
     CDS             8251..8523
                     /locus_tag="VFA02_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10981.1"
                     /translation="MARTPGGSWKACPSFGIRDLLEDARLRADAPRLALERANAALQE
                     LGFTEFTVEEIVREVSERRDYDVYSVTLVSGNERYTTTVQSEISEQ"
     gene            8520..10226
                     /locus_tag="VFA02_13845"
     CDS             8520..10226
                     /locus_tag="VFA02_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10982.1"
                     /translation="MSFQTPGILEIDHKLREDFRRRLKDFGITVDVLDPVLAVLFRTF
                     AHQIESVYANTGRIREKLLDELIAGIKLPGRHAHPAQTVVRFISSGPYAKTLRAGTEL
                     NAKAATGERLSFATDVSIELSPAQIAMALVYQEQQLQLLPGVDISEQFAAYRPSYDPV
                     RVNLGPQPAIFLAVENYGPSHLSHHGLFFDLSAGAYSIQQGLATEPWWFFGAEGDLSD
                     KGLMRPHRIHRGVRQLDWQVSSSSRARREENLPDLPDGFYAGRVFVFPEITEERKLLC
                     GVPRLLDVPLSRLAGHDLSSWFATPRIWIRIPLPSNVPALHTAIHGIILHATTASNVF
                     CLNQTVHFEQDGYSIPITRDHHGLRQMLVAPLSVSGLDNEIYHSAPGSAANPAEGWFE
                     ITKERITLHPGLKPDGSRQTGANVRLWLTNGGLGNLVAPGDITGFASSATFDQIRVNH
                     LTAAAGGTDDEDYSDARRRFSNALLTRGRIVTRSDLESTALSFDRRILAVDVRSEIER
                     GEHGLHRVERLWITLDADGFTRPEIELPILQEDLKRLLTARMLHGTHLDIQFEWKQGV
                     LA"
     gene            10223..11143
                     /locus_tag="VFA02_13850"
     CDS             10223..11143
                     /locus_tag="VFA02_13850"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZU10983.1"
                     /translation="MSTSALSRVMPDFVSGTHGFQHNLHSALACLARLQIPVSRIHIR
                     PVGWHSVPSGTVVGQSPQPGDPLEGIIQLQIAGPAFHHSLPVAMWDSGGEAEPGTREL
                     LEALDDPLVKLTHWMYEGASLLRLSPDNLAACARWLTLFGVSPEQWPRSLWYKLALLL
                     LELPSIAGSRQAVSIALDRVLDLPVKSSEFRSSLAVLKHPTRTRLGEKASQLGLNFIV
                     GDAFYDMAHLRVEIGPVSLPTYEKYLRAEGRELLDRLLTLVLPVHLDYELRWIVLDPE
                     QPPRLGIAEKNCRLQINSYLGKMHTFPDKL"
     gene            11201..12922
                     /gene="tssK"
                     /locus_tag="VFA02_13855"
     CDS             11201..12922
                     /gene="tssK"
                     /locus_tag="VFA02_13855"
                     /inference="COORDINATES: protein motif:HMM:NF017723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HZU10984.1"
                     /translation="MLLTPEHFLRLEQYFESLFYWGLRYMTSGHGLIGGGVRLPAPDL
                     GAVRLDPIVYLDENSEALNISVTQCRGLTPGGFLIDIDENGAIAERFDKESLAGVAET
                     EIYIVVDPSQRIKVDGVADEFNPQMRSERASSYRIALQVTAAERERSLAIARIRRPAS
                     GAHFEKDPVFIPPCMSLSAHSELMAGWRRIVESIQRLAQGYAELHRAMREFMVLFTER
                     GIETELDRDATQFAERMVLAFEEIAYSILDRTQSPQSFFTQVRRLLHQAATFFSLAPN
                     MQQYYETLRETGETELVSLIEGQKRHLDVTRTLKLADDLSLEVRSSLQSLAALEKLER
                     ALEGKYIDFRISPSLDGMNFIFDRGGKVLYKLAAKPSRVQGVADELTIFFSQLRLEGR
                     DRYRLILVGERDAPFSRGTTISAEIRLNEGTGFRREAIILSAEAKLDEQYNFEFDFEA
                     PDVPTITDLRVTVQAYHPVRTALLFIRHRFYASRTQEQTARNLEPEYERARPGNIPAP
                     MDTYTPPPAPAGNGRFSSVKPTETSQPSPVTEPVEETLPPWVPRDKRPTPTPEEPLTR
                     PRRRRLE"
     gene            12933..15593
                     /locus_tag="VFA02_13860"
     CDS             12933..15593
                     /locus_tag="VFA02_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU10985.1"
                     /translation="MQLDLSALSDAMEQALEQARLLAAERQQALIQPEHLLYTLLDHE
                     SSLYALLERNGIAVGPLLDALATRLNKATSGRLEPGRRPVASRALRNLLERSFALAAE
                     RNSPSVEPIDAVLAAVDQSMSDLRDDVRKDLREAGVTKEAVEKIEGTQRTLKETFQPE
                     KQPRASSAPVTEKVLERYGRDLTAEAAAGKIDPVIGRDDEIRSIIQTLLRKTKNNPVL
                     VGDPGVGKTAVVEGLALRIAAGDVPDSLRACRLYALDLPALVAGAKYRGEFEERIKSV
                     VEEVRSRKGEIILFMDELHQLVGAGGTEGGMDAANILKPALARGELRCIGATTFDEYR
                     ERIEKDGALARRFEQIQVKEPTDEAMVSILRGLRERYEQFHGVRLSDEALHAAVKLSR
                     RYIRDRFLPDKAIDVIDAATSRLRMQKESTPTHIDQQARLLTRKRAELEALRNGPQSA
                     QQKKAIPALEKEIAELAPQVEALTEAWQRARHASTELQKTVAAIEEQNRMLMQAEAAG
                     DVTKAAEIRYGSLKYLEQQRADLEAQASAASENPMVPTEVTAEHIAEVIGERTGIPIQ
                     RLLESERERLLHLEDRLSSRVFGQAAAVSAVSEAARRMRADLELRQTPNSFLFVGPTG
                     VGKTELAKALAEALFDDEHALIRIDMGEYKDKSSVSGLIGSRPGLVGSDEGGFLTEQV
                     RRSPFSVVLFDEVEKGHPEILDLLLGVLDEGRLTDAKGRFCDFSNTIVIFTSNLGVRE
                     AMASSEDPEERKNIILEIVRASLRPELYNRIGQIVAFDPLSEAELERIVKLHLSKLER
                     KLEEDHQLTLGVSPEAIAYLARHAYDPEYGARPAQRTIQQLVLSPLASLLLSEEPQPG
                     GKVAIALEEEDSLTFSLVGADQAEEVARTN"
     gene            15596..17734
                     /locus_tag="VFA02_13865"
     CDS             15596..17734
                     /locus_tag="VFA02_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HZU10986.1"
                     /translation="MRARERWKKWPGIAALVIGILLCSKYSLFSEPLTPAEAEEGWLR
                     LFDGTLTGWNANGNWQTKEEVLTSDMGDTRSIFTAYPLSDFFLKFDYRINATPSGAAL
                     RIRASHDGQPADSGYRIPLSDSAKDWPAGSIVRRSPAQAASIALNTWHSVTVEANGNH
                     IKVEIDGRPTADIHDDSSRAGYIGFESTRGAALDLRNLRARPLNVKQIFDGSDLSGWK
                     NVPYQHQAGKGLFHVFGGSGKTHSAEWSVKSGAIHGEKGPGAIETESQYDNFILQFKA
                     TADVDPNKKDVYPALSVRNQAGSIGTGYPLGISRHIGEIGSLAKPRKPVDNSKGDVLE
                     TVIVNGRSIFIFLNGALSTVYHDTRPEAASLKQGARVGSGVLSLEIPEDAHAVDIKSL
                     AAVSIPSGIGGLVKDQPPPQAAAPKTQTAPVVPADTAAQAAQLAAVLGVPTPQVRSRS
                     AELISRAVKSNDPQEQMDLYDQVIKMDPSNAAAIQGYKEAQQKLAAQQAQQEQQQQAQ
                     QAETQKESTNEQQLQNSLASAEASFLGGHIPEADRSLRIAERIAPSNPVVNDLRRRID
                     NALGLRRRLLFAGSAAGMAALLGGSVFFFRRRRLSRHPVLEVVQGLDQGRKYPLDRDV
                     VRIGAIAQDGGQKNDIVVRDVEHMVSRFHCEVVKKNGYLMLKDMNSSNGTKVDGKAVP
                     PGTLVPLGRSSRIDLGGTVVLRLGYERKKA"
     gene            17739..18452
                     /locus_tag="VFA02_13870"
     CDS             17739..18452
                     /locus_tag="VFA02_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZU10987.1"
                     /translation="MEIRIRAAGESHPGRRRRMNEDAFGFDLDRGLFVVCDGVGGNQA
                     GEVASRLALDTVLDHFHPGLNGSNGGSSLHRVVQKANHALRQAATREAAYSGMCTTIV
                     SAHLDASRMWIAHVGDSRAYLLRYGALHRLTEDHSLLAARLRSGLRTEAPELDGILTQ
                     ALGAGDFVVPEVTSVQAKKGDCFLLATDGLTRMLSNSEILSILVASLSPEHACHQLVE
                     AANRAGGFDNITCLVVAID"
     gene            18466..20700
                     /locus_tag="VFA02_13875"
     CDS             18466..20700
                     /locus_tag="VFA02_13875"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HZU10988.1"
                     /translation="MPNAGYIHVQIGEEDIDLALLAEVTVLQKLNAHWTAQVIFRSLH
                     DAQPDVAQMLGQTLKVSGYDLLGVEHTLFSGPVRDVELVYEVNGSYGARVDAISASWL
                     MDQSQRHAYFLKQSPSAIAQTLAGKYGLALKGPLEGDAGLSRVQWSESDWGFLLRLVD
                     DGEAWLRPTENGLETQTSFQPGPTLPWREGEYGLMEFSGAAKMRPVLQSGAHYDHRAA
                     QSQTYSGIKSEAAHYDPWSGLRGKTLAAGEALPAATVPGRHRATTLDSYQQQLQKESR
                     RQAVNATLCSGISRSPEVLAGNEVTLSGVDQDVDGTYGVIECLHHWTPRGYESHFKAT
                     TAKRWFAPERPARPQAEGVFPARVTANHDPQNLGRVQVQFYWQEENATTWARLITGHA
                     GAGRGLLITPEIGDEVAVRFEEADPERPYILGSVWNGVHLAPTQGLRGDEAPNNDIKR
                     LVTKSGHRLQISDRAGVETIALSTPTSNRLVMTEKANETGRPAVVLHTDGDIILAAPK
                     GRIHLQSMLQSREVGSTDGSDTTLKPGTSTGGQSGDTPRGRLQLPLPSHKPKTCKDAP
                     ETIHIPKHINRQMNDLWHQSFPGGNAQEHGGIIVKDSKGVYQMINIGSGQSGSFMPNR
                     NIPSGMTEVGLFHTHPYSQAEGGYTGVSFSGADIQIAATLNEPSYVQSGNRQFLIMPT
                     EETPTNLNYQQMNNAQNARIAELMEQGKTFQEAARIAANETAKTYKMAYYQGSNGVLH
                     RVSC"
     gene            20747..21370
                     /locus_tag="VFA02_13880"
     CDS             20747..21370
                     /locus_tag="VFA02_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10989.1"
                     /translation="MLRKATYTGTNDGYRVEIYAEGSGSLKARFVDKKTGFTESWQID
                     TDKQNWERLEALAKQASQLPAKTDGTSVSDEWFFDFNDGKSVRIASTHPEQDPSYLSF
                     REKAEQIFAAQYVVAKGFLDAGQRSASLQHWKVSAGQFKEGIAALGDSYVDTNALDDS
                     GMRLVLAQSDEKKGEVMRASRVYETVLLTRLHLYANKENIKDHSSIK"
BASE COUNT         5156 a         6115 c         5712 g         4600 t
ORIGIN      
        1 atcgttttcg tgttcagaag gctggcagat aagcgctggc atctttgcca ccgtaattaa
       61 aaatgcttcg tcgccgcctt gctccagagt taacagcttg tctggagatt cggcctgcaa
      121 ctgctgcaaa gcttcccatt catgagtaac tctgcgctgg cgaagcgtcg gcatattcac
      181 ccattggttg caatcgttga aatacgcaga acgtcattcg gtcgcactcc ctgttgatac
      241 aagggaatgg agaaatccag gctctggcca ttccagatga cctgtggttc cccttctcca
      301 gcgagcacat cgccgcgctc gcgcagatca aacaaaatct cttccagcac ctcggaaagt
      361 gtttcgtcct cgtcaatgtc aacgttatag cgtgtttccc cgcggacagt atcaaaatac
      421 atctggaatt tcggcatgat tatcgctcca tgtggaccgg cagatgcacg gcgacggtct
      481 cgagagattt tggcaggttc ggcatggtcc agccacagcg gctgcattcg ccgttggtct
      541 ggtcccagca gtcccggcat tgtggacgat ggcaatgtcc gcaactgcgc aagtcagact
      601 gctcaggtga ggccatacat aactggcagc gtccatgttg tacgcgcggc cccagttgtc
      661 ggtcccacca ctggctttga gcaggcagaa tgggaacgcg gttttcctcg cacatgcggc
      721 agcgcctgtg ttcaaaattc cagcagaact catcgcatac ttcaaggccg caacgcgggc
      781 atccaaacgt gattgcatgt ggccccagcc gtctgtggca caaggcgcag cggcgttttg
      841 tcatccggct taacagagcg gcaagagaaa tcactgcaaa cagaacaagg ggaagcgtcc
      901 acgtaactgt gccggtatgc cgggcagcag taagcacggc cagagagacg cagaaggttg
      961 cagtgagcac cgccggcagc atccaccatt gtccgaattg gacgtcgcac agagaggcaa
     1021 caaacggcgt ggcaattccg accaacagca ttaccacaat cagcgggaca ttgccggcaa
     1081 ttccggcaat caaaagcacc gtgaatgcga ttgccagcaa tccggcaact tgtgcaactg
     1141 ttcctgaacg tgccgtcata attcttttct accgggtatg gtaaaccggc gtgtacaaat
     1201 ggtcttcagc gtatgacaaa tctgccggag aaaattgcgc ttctccactg tttcttgtga
     1261 ttttatctat tgcttcgtat cggatccggg cagattctcg acactgctga catctgccat
     1321 atcgccttac ccaggccatc cggcgcaatg gttgccacct atggtgacac ctcaggcatg
     1381 ttgcatggat ggcaatgggc cagtcaagtt caaaggcatc tgcgtcaagc gacgtaaggg
     1441 aattctggaa tggttcattg taagcaagct ccaccagagt gttgctcgaa gaaaggtcat
     1501 gaaatgggca ggtagaagag tgcgataaaa cgtgccggac cagtccaggc ggatttccca
     1561 ggtctatctc ccatgcaaag gaactctctt ccttcgcgaa caggccatga cgaaatgcaa
     1621 gatctactgc catgcctgcc actacggatg ccatggttgg agtacttggc atatcacggt
     1681 tttcctccgc ccaccggcag ctcaaaacag gtgaacaggt cagggaaagt atttctgcac
     1741 ggcggttttc gcttagctgg cagcagtaac aggccgcatt tgtttctgcc ggaaaccatg
     1801 ctacccttcc gcgccagtat gccggaccgg acaagccggc atccaacata gggatacgta
     1861 gctttctgct tacataggcg gtttctaccc gcgacaaagc gctgtctgtg gcgctgatca
     1921 tcagcgcgca attcttcagg cccttaaaac ctgtatctgc aatttcggct acaagaggga
     1981 tccaccgggt acgcggaaaa taggtaagtc catgatttgc aattacttca gccttatagc
     2041 atcctaaagc gtccgagttg cggaaaaata tactttgcag actattgacg gattctactt
     2101 tgtcattgtc aactaatgcc acggttccga tcccgaggac cccaaggaga cgtgcaattt
     2161 cgctccccag ggcgccaagg ccaactaaag ccacggacag ggtttctatg ctgcaagact
     2221 ggtaactggt tcgctttagc tttgaaagac ccatttcttt cgcacaatac gcataacaac
     2281 aagattacag cggtctggtt tgtagaaggc taagtagttg atattttccg gtgaagacgc
     2341 ctgaaacgaa ttttaagttc gtttcacaac tatatttagt tctttatggc ctaatgaaat
     2401 tttgacagaa cgtatggatt tctttcgtct gatctatttt tcctccctct caggcgcgtt
     2461 ggccgggctc atggcatggg gcgttaacgc catgatcggt gcgattgtcg aaaccccatc
     2521 tgctgcatgg atggcagatg ccatactcct ggggttggtc gctgtcctgc tcattccatt
     2581 tcttctggca caactggacc gttattcggg gcgctctttt cgctggggga ctttatggat
     2641 tggcgtcctg attgccatcc tttcggcgct tattgcttcc ctgctgctat ggtttctccg
     2701 ctccaacatc gctgttgaat cgccattcct gtatcgcctt gccggttggt ctcttgccgc
     2761 ctccattctt ggcctcggga ttggctcccg ctggcttaca tcgaacaaac taagaatcct
     2821 gcacacctat acgggtggtc ttgccggggg tttattgggc gggttattat tcgtgggttt
     2881 tggcgggcac cagccagaac tttgccaggc ggctggcctt atgttgactg gagctggtat
     2941 tggcttcgga gcaggactgg ctccattatt gattcgggac ggcgcagtgc aattcatcag
     3001 cagcgcagat gcgcgggcgc aaagtaaata tggtcgcgcc aataaattct gggaattgca
     3061 gcacgaagaa acatacgtgg tcggaaacct tgccacatcg caatctgaaa cccgcttcca
     3121 gccaggagcg gaaattttca taccggacgc ctctatggcc gcccgtcatg ccgtgctctt
     3181 ttcaaaagaa ggacgctttt atattgccag acacccggat gcaggcggtc cggcaggcat
     3241 tgcgaaatac gtactcagag tacgtggcaa aactgtaaca acctcgcagg aactgacaga
     3301 agccgatgac attcttattg gccgcacagc gctgcgcttt actaccagaa agcagggaga
     3361 accatgagaa gcaagctgct catctgctgc tgtctcgttc ttgctgctgc aagcgcggct
     3421 gccgcgacac cggcatcttt gcgccagaca ggacctgcta ctttgcatga ctgctcgccg
     3481 gtcacccttg tcccctgctt caccgtgggc gtgggtcccg tagaccagaa cggcgctccc
     3541 gtcggggtcc ttcttccgcc tcaggaccgc ttgacgcagg ccgtttccat tcagacatcg
     3601 aacggggcga tcgcgccctt ttatgtaaag gcggcagctg gcggggacgc agtcgcgcgc
     3661 cccaatgtag tgctgattga aatagatatc agcgggagca tgaatgagac catcgctccc
     3721 ggcaccagcc gctttgctgc cgcaaagcag gccattcaga actacctcgc aatgatgcaa
     3781 gatggcgtgg atgaagtggc gattgttccc tttgagagcc atcatgttgt gcccaccatt
     3841 caggcggcgg tctttacttc caaacgcgac caggccgtgg cccagctcaa tgctctgccc
     3901 gaaccaggcc ctaagaacaa cacagcctta tatcaggcag tatttaccgg ggtagacacg
     3961 ctgaatgcgg agattgcgcg attggaaaag aaaggaacgc cgcgcggcaa tgttactccg
     4021 cagctcatcg tcatgacgga tggcaaaaac gaggtctttc atggcgatga tccagtgctg
     4081 ctcgatggtc cgctcggact ggagcaggct tcggcaaaag tccgtgatgc cggttacgaa
     4141 gtcataggaa ttggctttgg caatgcatcc gatattgatg cagaagcctt aaaaaagatt
     4201 tcaaccaggt atttcctggc gtcagacccg gcacagcttg ctcaggtctt tcgttcttcc
     4261 acaccggtcc acccctccga gcttgaaatt gcatttctcg cacctgtaac tgatcggcac
     4321 tcgctcgcgg cgcgcaatgc caatttcgtt gtaagtctcc atttgctgga cggaacagtg
     4381 ctgaccagcc cggcaatttc ctatgtcccg ccagccttgg gctttcctct gtatgaaacc
     4441 catcttgacc gcgaggccct ccaggcgctg gccgctgtag aaccccctgc catctcaggt
     4501 tggacaatcg ttctgcgggg catactggta ttttgcggtc tcggggtaat attgttgctg
     4561 ctatggttct gggtgccgcg tatcgtctgg aggggcggca ttcccgccgc aggcacccgc
     4621 cgccgctgga gcaaggaatc taccgtgcag gcttcgggag tacaggtaag agacaccccc
     4681 aatgggtttg acacggaagc tgctgccagt tccccgcagc gcaccgcggc ccaggtgact
     4741 caggtcaggc tccgcacgga cctgacaaat gcagctccac agagtggaaa caaaggtcaa
     4801 tgaggtttaa aggaaaagga gaataattcg atgccgctga ttaaagttgt tcgaaaggga
     4861 gtaacgccgc atgtaagaaa agtaccaact tctacgtgcg ccatgatgct tccaatgaca
     4921 cccgagcgta ttggagcaga agcggcttcc gaaaatgaag agccggtcaa aatcgaagac
     4981 ctgaatgatg cgttccgtaa attccagcca aagctgaaat ttaccggcca tgcaggagaa
     5041 gatgaggccg aattcaaggc cgagttcagg ttccgcagcg tcaaggactt cgacccggaa
     5101 aacctccaga aaaagcagga aattcgcaag gaagatggcg aagtggaata tctgcgcaac
     5161 gatattgcag acctcaagac caatatagat ctgctttatc gcctgaaaga ccgctggaaa
     5221 cttccgaccg tccggcgggc atgggccaat gaggcgcaac gtaaggaaat catcgccgcg
     5281 ctgggcaagt tgcgcagcga actggagcgg gtagcaaacg ggggtaaaca ataatggcag
     5341 acacgcagac cataacacgc acaaagacca cggaagaact tattgcagat tctcccgccc
     5401 gcgaactgtt gcaggacatc tttgcagacc tgcaccctgc gctgactgat gaaaagcgcg
     5461 agctggccag catctttact ccacagtctg ccaaagaatt tttgtcacaa ctgacaaact
     5521 tcgaccagct tctggatgaa aaagacagct ataacgactc ggtccagaaa ctacaacagc
     5581 agattgaaaa ggctgaatcc gtccgcgacg aattattgcg tcaggtgttt gagcagattc
     5641 gccccatcga acgcagctac cggcaactgc aactgttttt cgaaaacagt aaggtaatgg
     5701 acggcaaagt ccgcaaaccg gtcgaagttt acgttctgaa tgccgatccc acggccatga
     5761 aggacatctt cagcatgagc gttgcggcgg ttgaaaattt cgtgaaatct cgcaacgaca
     5821 acttcaattt ccgcgacgat atctgcaatc tggttgtacc gggcttttat ccgcagagca
     5881 tccgggaaaa atttgagcaa attgcaaacc aatggggcat gttgctgatc agcgatctcg
     5941 atgacgaaaa gtccttcagg aacgtggaga ggaacttcct tcccggcggg aaatacgaat
     6001 ttctgaagcg tcctgaggac caggccgcag ccgatgtgtg cctcatggga tatctgcagc
     6061 ttcgcgcccc ctattggttt gagaaagacc aatcgaatga tggcctgtat gctccaccat
     6121 ccgtagcatt tgccggcgct gttgcgcgtg cggatgacgc gcaggggctg gcgcaaggcc
     6181 cggttggctc ccgctttggc cgggtacacg gagtggaaaa ggcccgcatc gaaccgctga
     6241 ttggcgaaat ggagcacctc agcatggagc ggcagcttat tcccgtaatt cgcgacgccg
     6301 acaaccatct gtgcttcttc ggctgccgca cgcaggcgga cgacccatac ggtgtgctga
     6361 aatttttcac ctcctatcga attttgcgat accttgagcg gtgctgccgt cactatcttt
     6421 tgcaggtggc tgggcaggtg ctcacgcggg actttatgga ccagcagatt gaggcgccac
     6481 tgaagcgcct gctggatgaa caggtcgagc aggggaccat tctcagctat gacctgtttg
     6541 tagacaaaga ttcaaacaaa cgtatgcaag gcatctgtga catcacattg agcgtgatgc
     6601 caacaggccc tgcagagact tttgtgctga agattgatgt tccggaattc agtaaaccca
     6661 cgaatcctga agcaaaacag gcaaacgcat agaaatgttg gaaagctgaa gattcactat
     6721 gtctttgggg ccgcacaaga agagcatcga gccaggagac agcgcgcgca gggccgaaca
     6781 ggggccgcat cctgcgccat tccgccgtat tgacggaggg acggcagcgc gcgatgccga
     6841 gcagcgcagt tacgacacct ccattgggcg gaccgttcac gggtttactt tgtctgctag
     6901 agtaggcaag ccattcaaat tcgagaatgg tgtgcctgcg gctgccggag aagcgatgcc
     6961 tgcggagtca ccggcatccg ctccggagat ccagatcaaa aagacaaatc aaacacaaaa
     7021 tgaaaaacca atgaatgttg ctgctcctga aaagccgcat gtagcagatt catctgcgat
     7081 ttccttccct ggttcttttt tgagagcact cttccggcgg cggtttccgt ccgcatcgcc
     7141 agaaaatgat acgtagttcc aattctgtga gtaggagaga acagcgtggc agcatcacca
     7201 tatcgcacga cagttacgat tgatggcaca aagtttgacg ccatctctac gagtgtccgg
     7261 ttcagtacgg aaaaagaccg gtcgggtgta ccgcaaatgg gcagtctggc aacatcggtc
     7321 cgagtatggg ccgattttca cgatgatcaa aacattcctt tcagcaccgt ccagaagctg
     7381 tttaatctgg caaacgttgt gacgcgcgac aagatccgct ccatcaaaat tgaatactgg
     7441 aaagacgact ccaagcagga cgccctcgtc agctacagtt tcaatggctg gatccgccgc
     7501 tttgaaacca gcaatccgct cgactttgtc cgggccactt ccaccagcag cgatgaagat
     7561 aacttcgtca gcggcatcta cagcaacacc aaccatctgc ttgttctgga tatcgaacct
     7621 gctctcaatc agcagaactt ccagaacgtt caggtcagca actaggaaag gagacacaca
     7681 catggccgct tcaccaaacc gcagcacact tacgattgat gggaataagt ttgactgtgt
     7741 ttccgtctcc gttgcgctcc agactgaaaa agaccgcgct ggcatgcccc aaatgggaag
     7801 cctcagtacg tccatcaaag catatgttga tttccacgat gatcagaatc tgcctttcag
     7861 caccctgaag aagctctttg atctttccaa tgtagttacg cgcgacaaaa tccgtgacgt
     7921 aaaaattgaa ttctggaaag acgatgccaa acaggatgcc ttggtgagtt acaactttaa
     7981 gggttggatt tccagcttcc atactttaaa tccataccag aatggtcaac catccgataa
     8041 cgaccatatc ggagtgaacc atatgctggt gctggaactg gagcctgtcc tgaatcagca
     8101 gaactttaag gacgtcacca tcagcaacta agcccggtat aagaagaaac ggggacgcag
     8161 ttgtttctct cttttccatt gcgagtagag gaaggcggat ttctccacag atcggacagg
     8221 tccgccgcta tcctctttct catgcagttg atggcaagga cccccggcgg ctcctggaaa
     8281 gcgtgcccaa gctttggtat tcgcgacctg ctggaggacg cgagattacg cgccgatgcc
     8341 ccacgccttg ctctggagcg cgcaaatgcc gcacttcagg agcttgggtt tacagagttc
     8401 acggtagagg agattgtacg ggaagtctcc gaacgccggg actacgatgt ttattccgta
     8461 acgctcgttt caggcaatga acgctatacc acaaccgtgc aatcagaaat ttccgagcaa
     8521 tgagttttca aacgccaggc attcttgaga ttgatcacaa gctacgggaa gactttcgcc
     8581 gccgcctgaa ggattttggc attaccgtag atgtcctcga tccagtgctt gcggtcctct
     8641 tcaggacttt tgcccatcag atagagtcgg tatacgcgaa tacaggccgc attcgtgaaa
     8701 agctgttgga cgaactgatt gcaggcatca aattgcccgg acgtcatgct catcctgcgc
     8761 aaacagttgt gcgttttata tccagcgggc cttatgccaa gacccttcgt gccggcactg
     8821 agctgaatgc aaaagctgca acaggcgagc gcctgagctt tgctactgat gtcagtatcg
     8881 aattatcacc cgcgcaaatc gccatggccc tggtctatca ggagcagcaa ttacaattgc
     8941 tgcccggcgt tgatatttcc gagcagtttg ctgcttaccg accctcctat gatccagtca
     9001 gagtcaatct cgggcctcag ccagcgattt tccttgctgt cgaaaattac gggccgtcac
     9061 acctcagcca tcacgggctt ttctttgatt tgagcgctgg cgcttattcc attcagcaag
     9121 ggctggcaac agaaccctgg tggttttttg gtgccgaagg agacctcagc gataaaggac
     9181 tgatgcgtcc tcaccgcatc catcgcggag tgcggcaact ggattggcag gtgtcatctt
     9241 cctcacgcgc gcgtagagaa gaaaatctcc ccgacttgcc agatggtttc tatgcaggca
     9301 gggtttttgt tttccccgaa attaccgagg agagaaagct cctctgcggt gtacccagac
     9361 ttctggacgt cccattgtcc aggcttgccg gacacgactt gagttcctgg tttgcaacac
     9421 cccggatatg gatccggatt ccacttccct ccaatgtgcc agcactgcat accgccatac
     9481 acggcatcat ccttcatgcc acgactgcat cgaatgtgtt ttgcctgaac cagactgtcc
     9541 attttgaaca ggatggttat tccattccca tcacccgtga ccatcacggc ttgcgtcaaa
     9601 tgctggttgc gcctctttcc gtttccggcc tggataacga gatataccac tctgctcccg
     9661 gaagcgcggc aaatccggca gagggctggt ttgaaattac gaaggagaga atcacgctgc
     9721 atcctgggct taaaccagat ggctccagac agacaggcgc aaatgtgcgc ctttggctca
     9781 cgaatggtgg ccttggcaat ctcgtagctc ccggagacat caccgggttt gccagcagcg
     9841 caaccttcga ccagatacga gtcaatcatc tgactgccgc cgcaggagga acagatgatg
     9901 aagactactc cgatgcacgc cgccgctttt ctaatgccct gctcacacgt ggccgcattg
     9961 tcacccggag cgaccttgag tccacggcct taagttttga tagacgcatt cttgcagtag
    10021 atgtaagaag tgaaattgaa cgcggagagc atgggctgca tcgcgtggaa cggttgtgga
    10081 tcacgctcga tgcagacgga tttacccgtc ctgagattga attgcccatt ttgcaggaag
    10141 atctgaagcg ccttttaacg gcacgtatgc tccatggcac tcatctcgac atccagtttg
    10201 agtggaaaca gggggtgctt gcatgagcac ctctgccctt tcccgtgtca tgcctgattt
    10261 tgtttcaggc acacatggat tccagcacaa cctgcattcc gcccttgcct gtctcgcccg
    10321 tttgcagatc cctgtctcgc gtatccatat ccggcccgtc ggatggcaca gtgttccgtc
    10381 tggtactgtg gttggacagt ctccccagcc aggagatccg ctggaaggaa tcattcagct
    10441 tcagattgcc gggcctgcct ttcaccacag tcttcccgtc gccatgtggg acagcggagg
    10501 cgaagcagaa ccagggaccc gcgaactgct cgaagcccta gacgatcctc tcgtaaagct
    10561 gacccattgg atgtatgagg gagcatcgct gctgcgcctt tcgcctgata atctggcggc
    10621 gtgcgcgcgc tggctcaccc tgtttggcgt ttctcctgag caatggccac gttctctctg
    10681 gtataaactg gcccttctgc tcctggaatt gccttcgatt gcaggaagcc ggcaggctgt
    10741 aagcatcgcg ctggaccggg tactggatct gccggtgaaa tcatctgaat ttcgcagctc
    10801 gcttgccgtg ctgaagcacc ccaccaggac ccgcctgggc gagaaagcat cgcaactggg
    10861 cctgaatttt atcgtcggcg acgcatttta tgacatggcc cacctgagag tagaaatcgg
    10921 gcctgtgtcc ctgcccactt atgaaaagta cctgagggct gaagggcgcg aattactcga
    10981 ccgcctgctg acgctcgtgc tccctgtcca cttggattac gagttgcgct ggatcgtgtt
    11041 ggaccctgaa cagcccccgc ggttggggat tgctgaaaag aactgccgct tgcagatcaa
    11101 tagttatctc ggcaaaatgc atacctttcc agacaagttg taagaaatcg aggaagagat
    11161 ggacccgctt cgtttgagat cagtcaattg ggaacacgga atgctcctga caccggaaca
    11221 tttcctgcgc ctggaacaat attttgaatc gctgttctac tggggactgc gttatatgac
    11281 ctcaggtcac ggtctgattg gcggtggcgt gcggctccct gcccccgatc ttggcgcagt
    11341 caggctcgac cccattgtct atctggacga aaacagcgag gccttaaaca tctccgtcac
    11401 ccagtgccgc gggcttacgc ctggcggttt tctgattgat atagacgaaa atggggccat
    11461 cgcggaaagg ttcgataaag aatctcttgc cggcgtcgct gaaacagaaa tttacatcgt
    11521 cgttgatccg tcccaacgaa tcaaggtgga tggcgtcgcg gatgaattta atccacagat
    11581 gcgtagcgaa cgtgccagca gttaccgcat cgcattgcaa gtgacggctg cagagcgtga
    11641 acgctcactc gccatcgccc gcattcgcag acctgccagc ggggcgcact ttgaaaaaga
    11701 cccagtattt atcccaccct gcatgagcct ttccgcgcac agcgagttga tggcgggatg
    11761 gcgcagaatt gtggaatcca tccagaggct ggcacaaggc tacgcagagt tgcaccgcgc
    11821 catgcgcgag ttcatggtgc tatttactga gcgtggcatc gagactgaat tggatcgcga
    11881 tgcgactcaa tttgccgagc gcatggtcct ggcctttgaa gagattgcct atagcattct
    11941 cgaccgtacg caatcgccac agagtttctt cacgcaggta cgcagactgc tgcatcaggc
    12001 agcaacgttc ttcagccttg ctccgaacat gcagcaatac tacgaaaccc tgcgtgagac
    12061 cggagagaca gaactcgttt ctctcatcga aggccagaaa cggcacctgg atgtgacccg
    12121 gacgctaaag ctggccgacg acctcagtct ggaagtgcgt tcgtcgctcc agtcgcttgc
    12181 ggcactcgaa aaactcgaac gcgctctcga aggaaaatac attgacttcc gcatcagccc
    12241 atcgctcgat ggcatgaact ttatcttcga ccgcggcggc aaagtactct acaaactggc
    12301 tgcaaaacca agccgcgtgc agggtgttgc agacgaatta accattttct tctctcagtt
    12361 gcgcctggaa ggccgcgacc gttatcgtct cattcttgtc ggagaaagag atgcgccgtt
    12421 ctcgcgtggc acaaccatca gcgccgagat tcgcctgaac gagggtacag gtttccgccg
    12481 cgaagccatc attctttccg cagaagcgaa gctcgatgag caatacaact tcgagtttga
    12541 ttttgaagca cctgatgttc cgaccatcac cgatttacgc gtgactgtgc aggcctatca
    12601 tcccgtccgg acggcgcttc tgtttatccg gcaccggttc tatgcgagcc gcacgcagga
    12661 acagacggcg cgtaatcttg agcctgagta tgaaagggcc cggcccggca acattcctgc
    12721 cccgatggac acttatactc ctcctccggc accggcaggc aacggccgct tctcatccgt
    12781 aaaaccgacc gagacatcgc aaccctcccc ggtcaccgaa ccagtggaag aaactttgcc
    12841 tccgtgggtc ccgcgagaca agcgcccaac gcccacgcct gaagaaccat tgacacgccc
    12901 acgacgcagg cgcctggagt aacggagcac tcatgcagct agacctcagt gcattatcgg
    12961 atgcgatgga acaagcgctc gaacaggcgc gactacttgc cgccgaacgg cagcaggcgc
    13021 tgattcagcc tgaacatctc ctctacacct tgctcgatca tgagagcagt ctctatgctt
    13081 tgctggagcg caatggcatc gcagtcggcc ccttgctgga tgcgctggca acgcggctga
    13141 acaaagccac ttctggcagg ctcgaaccag gacgccgccc tgttgcttcc agggccctgc
    13201 gaaatctcct ggagcgcagc tttgctctag cagccgaacg caactctccc tccgtagaac
    13261 ccattgatgc cgtgctggcc gccgtggacc agagcatgag cgatctgcgc gacgatgtgc
    13321 gcaaggacct gcgcgaagcc ggagtcacca aagaagcagt agaaaaaatc gaaggcacac
    13381 agcgcacttt gaaagagacc tttcagccgg aaaaacaacc gcgcgcctcc agtgcccccg
    13441 tcacagaaaa agttctcgaa cgctacgggc gcgacctgac cgcggaagcg gccgcaggaa
    13501 aaattgatcc ggtcattggg cgcgatgatg aaatccgttc gatcatacag acccttctac
    13561 gtaaaacgaa gaacaatcct gtcctggtag gcgaccccgg cgtcggcaaa acggctgtcg
    13621 tcgaaggact ggcgctgcgc attgccgcag gagatgtgcc ggatagcctg cgtgcctgcc
    13681 gcttatacgc attggatttg ccggcattgg tggctggcgc gaaatatcgc ggcgaatttg
    13741 aagagcgcat taaatccgta gtggaagaag tacgttcgcg caaaggcgaa atcatcctat
    13801 tcatggacga gctgcaccag ttagtcggcg cgggcggtac cgaaggcggt atggatgctg
    13861 ccaatatcct caagcctgcc cttgcccgtg gcgagctgcg ctgcataggg gccacgacat
    13921 tcgatgaata ccgtgagcgc atcgagaaag atggtgcgct ggcccgccgt tttgagcaga
    13981 tacaggtcaa ggaaccaacc gacgaagcca tggtctctat cctgcgtgga ttgagagagc
    14041 gctatgaaca atttcatggt gttcgccttt ctgatgaggc cctgcatgcg gcagtaaaat
    14101 tatcgcgccg ttacatacgc gaccgcttcc ttcccgacaa ggccatagac gtaattgacg
    14161 ctgctacctc ccggctgcgg atgcagaaag aatccacgcc aacacacatt gaccagcagg
    14221 cgcgtctgct cacgcgcaag cgcgccgagc ttgaggccct gcgcaacggt ccgcagagcg
    14281 cgcagcagaa aaaagcaatt ccagcacttg aaaaagaaat tgctgagctt gctcctcagg
    14341 tagaagcgct gaccgaagcc tggcagcggg cgagacatgc ttccaccgaa ctacagaaga
    14401 ccgtggccgc catcgaagag cagaacagga tgcttatgca ggccgaagcc gcgggtgatg
    14461 taactaaggc cgcggaaatc cgttacggca gcctgaaata tcttgagcag cagcgcgccg
    14521 atctggaggc tcaggcgagc gccgctagcg agaaccccat ggttcccacc gaggtcaccg
    14581 ccgaacacat cgcagaagtc atcggcgagc gcaccggcat tcccattcag cgccttctcg
    14641 aaagcgagcg cgaacgtctg ctgcacctgg aagaccgcct ctcttcccgc gtattcggac
    14701 aggccgcggc cgtcagcgcc gtatcggaag cagcgcgccg tatgcgtgct gacctggagt
    14761 tgcgccagac acctaattcc ttcctctttg ttggcccaac tggtgtcggc aaaaccgaat
    14821 tggccaaggc cctcgcagaa gcactgtttg atgacgaaca cgccctgatt cgcattgaca
    14881 tgggcgaata caaagataaa tcctccgtct ccggtctcat tggctctcgt cctggcctgg
    14941 ttggttctga tgaaggtggt ttcctcaccg aacaggtgcg ccggtcgcct ttctccgtcg
    15001 tgctgttcga cgaagtagaa aaaggccatc cggaaattct cgatcttttg ctgggcgtgc
    15061 tcgacgaagg tcgcctcaca gacgcaaagg ggcgcttctg tgatttttcc aacaccatcg
    15121 tcatcttcac ctcgaacctg ggtgtccgtg aagccatggc cagcagcgaa gatcctgaag
    15181 agcgcaagaa catcatcctg gaaattgttc gcgcaagcct gcgcccggaa ctttataacc
    15241 gcatcggaca aatcgttgct ttcgatccgc tttctgaagc tgagctggaa cgcattgtga
    15301 agctgcacct gagcaagctg gagcgcaaac tcgaagaaga ccaccagttg actctgggcg
    15361 taagcccgga agccattgcc tatcttgcca gacatgcgta tgaccctgaa tatggcgcac
    15421 gccctgcaca gcggaccata cagcagcttg tgctctctcc gcttgccagc ctgttgcttt
    15481 cagaagaacc gcagcctggc ggcaaggtcg ccattgcgct tgaagaggaa gacagtttga
    15541 ctttcagcct ggttggagcc gatcaggctg aagaagtagc gaggacaaat tagccgtgcg
    15601 tgcaagagaa cgctggaaaa aatggccggg catcgctgcc ctggtaatcg gcatattgct
    15661 gtgctcaaag tattcacttt tttctgaacc gcttacgccc gctgaagcag aagagggctg
    15721 gctgcgtctt tttgacggca ccctcactgg ctggaacgca aacggaaact ggcagacaaa
    15781 agaagaggtg ctgacttccg acatgggaga tacccgcagc atcttcaccg cctaccctct
    15841 cagtgatttc tttctcaagt tcgattaccg catcaatgcc acgccctccg gcgcggccct
    15901 gcgcattcgc gccagccatg atggccaacc cgcagacagc ggctaccgta tccctctttc
    15961 cgattcagca aaggactggc ccgcgggcag catcgtcagg cgcagcccgg cccaggctgc
    16021 ttctatcgcg ctgaatacat ggcacagcgt tacggttgaa gcgaacggca atcatatcaa
    16081 agttgaaatt gacggccgtc caaccgccga cattcatgat gactcctctc gcgccggata
    16141 catcggcttt gaatccactc gtggcgctgc actcgacctg agaaacctcc gcgccagacc
    16201 tctgaatgta aaacaaatct ttgatggttc cgacctctcc ggctggaaaa atgtccccta
    16261 tcagcatcag gccggcaaag ggctgttcca cgtcttcggc ggatcaggca aaacacatag
    16321 tgctgaatgg agcgtaaagt ccggcgccat tcacggcgaa aaaggccccg gcgccatcga
    16381 aaccgaatca cagtacgaca atttcattct ccagttcaaa gcaacagccg atgttgatcc
    16441 gaacaagaaa gatgtttacc ccgcactctc tgttcgcaat caggccggaa gcatcgggac
    16501 cggatatcca cttggaatca gcaggcatat tggggagatt ggttctctag ccaagcctcg
    16561 caagcctgtg gacaacagca agggcgacgt tcttgaaaca gtcatcgtaa atggccgctc
    16621 catatttatc tttctgaacg gcgcgctctc caccgtctat cacgatacgc gtcctgaagc
    16681 agcttcttta aaacaaggtg cgcgcgtagg cagtggcgtg ctcagccttg aaatcccgga
    16741 agacgcccac gccgtagaca taaagtctct tgccgctgtt tcgatccctt ccggtattgg
    16801 cggcctcgtg aaagaccaac ccccgccgca ggctgctgca cccaaaaccc agactgctcc
    16861 agttgtccct gctgacacag ctgcgcaggc agcgcaattg gccgcagtgc ttggagttcc
    16921 cactccccag gtgcgctcac gctctgctga acttatcagc cgcgcggtaa agagcaatga
    16981 tccacaagaa cagatggatc tttatgatca ggtgatcaag atggatccgt ccaacgctgc
    17041 cgctattcag ggctacaagg aagcacaaca gaagctcgcg gcgcaacagg cccagcagga
    17101 acagcagcaa caagcccagc aggctgaaac acagaaagaa agtaccaacg aacagcaatt
    17161 gcaaaacagc ctcgcctccg ctgaagccag cttcctcggt ggccacatcc ccgaagccga
    17221 ccgctcctta cgcatcgcgg aaagaatcgc cccctccaat ccagtagtaa atgacctgcg
    17281 ccgccgcatt gataatgccc tcggcctgcg ccgcaggctc ctctttgccg ggagcgccgc
    17341 aggcatggcc gcgctgctcg gcggctctgt cttcttcttc cgccgccgca gactcagccg
    17401 gcaccctgtt cttgaagtcg tccagggcct tgaccagggc cgcaaatacc cgctcgaccg
    17461 cgacgtggtc cgcatcggtg ccatcgcgca ggatggtgga cagaagaacg acattgtggt
    17521 ccgcgatgtg gaacacatgg tctcgcgctt ccattgcgag gtcgtgaaaa aaaatggata
    17581 tctcatgctg aaagacatga acagctccaa tggaacgaag gtggacggca aagcagtgcc
    17641 gcccggtacc ctcgttccac tcggcagaag ttcccgcatt gacctgggtg gcaccgttgt
    17701 gctgcgcctc ggctacgaac gcaaaaaggc atagctctat ggagatccgt atccgtgcgg
    17761 ctggcgaaag ccatccagga cgcagacggc gaatgaatga agacgccttt ggctttgatc
    17821 tcgaccgcgg cctctttgtc gtatgtgacg gggtcggcgg caaccaggcc ggtgaggtcg
    17881 caagcagact cgccctcgat accgtcctcg accactttca ccccggactg aatggcagca
    17941 atggaggctc ttccctgcac cgcgtcgtcc agaaagcaaa ccacgctctg cgtcaggccg
    18001 ccacgcgcga agccgcctac tccggcatgt gtaccaccat tgtttctgcg caccttgacg
    18061 ccagccgcat gtggatcgcc catgtgggag acagccgcgc ctacctgctt cgttatggcg
    18121 cgttgcaccg cctcacagaa gaccattccc tgctcgccgc gcgccttcgc agcggattgc
    18181 gcaccgaagc gcccgagctt gacggcattc tcacacaggc actcggcgca ggagatttcg
    18241 ttgttccgga agttacatcc gttcaggcaa agaaaggcga ctgcttttta cttgccaccg
    18301 atggcctgac cagaatgctc tccaattccg aaatcctgag catcctggtc gcttcccttt
    18361 ctcctgaaca cgcctgccat caattggtgg aagcagccaa tcgcgcaggt ggtttcgaca
    18421 acattacgtg cctcgtagtt gcgatagatt gacggaggac atccagtgcc gaacgctggc
    18481 tacatacacg tacagattgg cgaagaagac atagacctcg ccctgctcgc agaagtgacg
    18541 gtcttgcaga agctcaatgc gcactggact gcgcaggtca tcttccgctc cttgcacgac
    18601 gcgcagccgg acgtggccca gatgctcggc cagaccctga aggtctccgg ctatgacctc
    18661 ctcggcgttg aacataccct gttcagcgga ccggtgcgcg acgtcgagct tgtctatgaa
    18721 gtcaacggca gctacggcgc ccgtgttgac gccatcagcg caagctggct catggaccag
    18781 agtcagcgcc acgcctattt cctcaaacag tctccctccg ccatcgcaca gacactcgct
    18841 ggaaagtacg gcctggccct caaaggccct ctcgaaggcg atgccggcct ctcgcgcgtg
    18901 caatggagcg agagtgactg gggctttctg ctgcgtctcg tagatgatgg cgaagcctgg
    18961 ctccgcccca cagaaaacgg tctggagacg cagaccagct tccagcccgg cccaacgctt
    19021 ccgtggcgag aaggcgaata cgggctgatg gagttctccg gcgctgccaa aatgcgtcct
    19081 gtcctgcagt ccggagccca ctatgaccac cgcgccgccc agagccagac ctactcgggc
    19141 atcaaaagcg aagcagcaca ctacgacccg tggagcggcc tccggggcaa gaccctggcc
    19201 gcgggcgagg cccttcctgc agccaccgtc cccggacgcc atcgtgccac cacgctcgat
    19261 tcgtatcagc aacagttaca aaaagaatcg cgccgccagg ccgtcaacgc aaccctctgt
    19321 tccggcatca gccgctcgcc ggaagtcctg gccggaaatg aagtcaccct ctccggcgta
    19381 gaccaggacg tggacggcac ctacggcgtc atcgagtgcc tgcaccactg gacgccgcgc
    19441 ggctacgaaa gccacttcaa ggccaccacg gccaaacgct ggtttgctcc cgaacgcccc
    19501 gcccgtccgc aggccgaagg cgtcttcccg gcccgcgtca cagccaacca cgacccgcag
    19561 aacctcggcc gcgtgcaggt gcagttctac tggcaggagg aaaacgcaac cacctgggca
    19621 cgcctcatca ccggccatgc cggagcaggc cgcggacttc ttattacgcc tgaaatcggt
    19681 gacgaggtcg ccgtgcgctt tgaggaagcc gaccccgagc gcccctacat cctcggctca
    19741 gtctggaacg gagtacatct ggcccctaca cagggcctgc gcggagatga ggcaccgaac
    19801 aacgacatca agcgcctggt aaccaaaagc ggccatcgcc tgcagatcag cgacagagcg
    19861 ggagtagaaa ccatcgccct gtccaccccg acgagcaacc gcctggtaat gaccgaaaaa
    19921 gccaatgaaa caggccgccc cgccgtagtg ctccacaccg acggcgacat catcctcgcc
    19981 gccccgaagg gcagaatcca cctgcaaagt atgttgcagt ccagggaagt gggttccacc
    20041 gatggcagtg acacaactct aaaaccgggc acatcaactg gaggacaatc gggagacaca
    20101 ccacgaggaa gactccaact tccgttgcct tcgcataaac caaaaacatg caaagatgct
    20161 ccggaaacaa ttcatattcc caagcacata aaccgccaga tgaatgatct ttggcaccag
    20221 agcttcccag gcggaaacgc tcaggaacat ggtggaataa ttgtgaagga ttcgaaaggt
    20281 gtgtaccaga tgattaatat aggttctggg caatcaggaa gctttatgcc gaaccgtaat
    20341 attccttcag gaatgactga agttggcctt tttcacacac atccatattc gcaagcagaa
    20401 ggtggatata cgggcgtctc ctttagtgga gcagacattc aaatagcagc tactctcaat
    20461 gagccttcgt atgttcaaag cggaaatcgc caatttttaa taatgccaac tgaagaaaca
    20521 ccaacgaacc taaactacca acaaatgaat aatgcacaga acgcaagaat cgcggaattg
    20581 atggaacaag ggaaaacatt ccaggaggca gctcgtattg cagcgaatga aaccgcaaag
    20641 acatacaaaa tggcatatta ccagggcagc aacggtgttc ttcatcgcgt tagctgctga
    20701 gattatcgct ccgaattttt catactcttt aacggagaac gggactatgc tgcgcaaagc
    20761 aacctacaca ggaactaatg atggttatcg cgtcgaaatt tatgccgaag gatccggaag
    20821 tctcaaggcc agatttgtag acaagaaaac tgggtttaca gagtcttggc agattgacac
    20881 cgataaacaa aactgggaac gattggaggc tctcgctaaa caagcttcgc agctgcctgc
    20941 caagacggac gggacttccg taagtgatga atggtttttc gacttcaatg atggtaaatc
    21001 cgtacgaata gcatccaccc atccagaaca agacccatct tatctttcct tccgtgaaaa
    21061 agctgagcag atttttgcag cgcaatacgt tgtagcaaaa ggctttttag acgccggaca
    21121 aagaagtgct tctcttcaac attggaaagt ttcagcgggt caattcaagg aaggaatcgc
    21181 agctttaggc gattcttatg ttgacacaaa tgcacttgat gattcgggaa tgaggctagt
    21241 gctggctcaa tcagatgaga aaaaaggtga ggtcatgcgg gcgtcaagag tatacgaaac
    21301 agttttgcta acacgtcttc atttatacgc aaataaagaa aatattaagg atcatagttc
    21361 aataaaatag cccttcaggg tcgccagaaa agatggagcg ggccataggt atggcggata
    21421 tacctcaggc actactagtc ttctgagtca taagtaattt gcataaatgt gagggatgat
    21481 ctaagttgag gtatggagtc tggtcgtggg cgtccgaaga agcaggaact gattgttaca
    21541 gaaaaacagc ggagtgatct cagtcgtctg acggcgcaag cgc
//
LOCUS       DASRBV010000380         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_184752, whole genome shotgun sequence.
ACCESSION   DASRBV010000380 DASRBV010000000
VERSION     DASRBV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184752"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFA02_13885"
     CDS             <1..1153
                     /locus_tag="VFA02_13885"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZU10990.1"
                     /translation="QMPGLDAFDRWSIDRDHHILNARSVQYVGRGVVGYDDLDDYGSW
                     ADTPEYGPVWYPASVAVDWVPYRYGHWVWIGPWGWTWVEEEPWGYAPFHYGRWALIGS
                     RWGWVPGPVVAVPVWAPAFVVFAGGDGFGAGISAWFPLGPGEPFYPWYHCSPAYIRNI
                     NITNINITRIRNVTVVNNYNYFINHVNNVNNVHINYANRERAFTAVNSSAFASARPVH
                     ENLVHVDPQQINRAQVIPHPMVSPTLEAKVPHPVRAVAAPVARPMVQVHARTAVPIQN
                     PVQTPAPHPQPVAPVARGPQRTLTTTNQPPRPVEPRPLIGRTPPPPQGPSFEQRQQAM
                     QAHPGRPLEPQQIENLRQGRPAGQMQDREWPPHPQPMARPEARPQPPRR"
     gene            complement(1203..2618)
                     /locus_tag="VFA02_13890"
     CDS             complement(1203..2618)
                     /locus_tag="VFA02_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZU10991.1"
                     /translation="MSRSRITRRDLLRNAGASGVLALGLPAAGQNQPQPPPVKPGQQP
                     QTEEQAAQQYQRGVGVFINRPLTQVSKQADALLDDLEGRACDYFYNEAHPKTGLVRDR
                     APVAGRSLGRVASIAATGFGLTALCIASKRNYLPRLQCEERVEKTLAFLLEDCPHEHG
                     FLYHFIDMESGQRMFGSELSSIDTALLLCGVLTCRQYFSGNARIEALATTFYRRIDWQ
                     WMLNGGTTLSMGWLPDLGFLKHRWDIYSELMTMYLLAIGSPTHPIPSETWNALQRPLV
                     QFGGINYISGLAPLFIHQYAHAWCDYRNQRDLHTNYFTNSIAATRVHQLWCMTLGGKF
                     SWIDQDLWGISASDSREGYRVWGGPPAIGPLDGTVVPCACAGSLPFLPAECSHVLLTM
                     REKYGEKVWTRYGFVDAFNPKANWYAPDILGIDQGISVLMAENLRTGFVWEQFMKNRE
                     VAHAMREVQFHPDPDVNSQVL"
     gene            2763..>3049
                     /locus_tag="VFA02_13895"
     CDS             2763..>3049
                     /locus_tag="VFA02_13895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU10992.1"
                     /translation="MISIRSSTLKDADVVWEILEPVIRDGETYTLPRDLTREQALQYW
                     YSPQHEVFVAGKDGEVLGTYYLRANQQGGGSHVANCGYMTASRARGQGVASA"
BASE COUNT          638 a          841 c          942 g          628 t
ORIGIN      
        1 gcagatgccc ggtctcgatg cgtttgaccg ctggagtatt gaccgcgacc accatattct
       61 gaatgcgcgc tctgtgcagt atgtaggccg gggcgtggtt ggatacgacg atctggatga
      121 ttacggaagc tgggccgata ccccggagta tggaccggtg tggtatccgg catcggttgc
      181 ggtggactgg gtgccgtatc gctatggtca ttgggtgtgg attggcccct ggggatggac
      241 gtgggttgaa gaagagccct ggggctatgc gccatttcac tatggacgct gggccctgat
      301 tggttcgcgt tggggatggg tgcccgggcc tgttgtcgct gttccggtat gggcgcctgc
      361 atttgtggtc tttgcaggag gcgatggttt tggagcgggc atttccgcgt ggtttccgct
      421 tgggccgggc gagccgttct atccgtggta tcactgcagc cctgcgtaca ttcgcaatat
      481 caatattacg aacatcaaca tcacgcgcat tcgcaatgtg actgtggtga acaactacaa
      541 ctacttcatc aatcatgtga acaacgtgaa caatgttcac atcaattatg cgaaccggga
      601 gcgcgcattc acggctgtga acagttcggc gtttgcgtcg gcgcgtcccg tgcatgaaaa
      661 cctggtccac gtagacccgc agcagatcaa tcgcgcacag gtcattccgc atccaatggt
      721 ttcgccgacg cttgaagcga aggtgccgca tcctgtgcgt gcagtggccg ctcctgtggc
      781 ccgtcctatg gtgcaggtgc atgcgcggac tgcggtccca atccagaacc cggtacagac
      841 cccggcgccg catccgcagc cggtcgctcc agtagcgcgc ggaccgcagc ggaccttgac
      901 gacaacgaac cagccgccac ggcccgtgga gccgcgaccg ctgattggac ggacgccacc
      961 gccaccgcag ggaccttcct ttgagcagcg gcagcaggca atgcaggcgc atccagggcg
     1021 tccgctggag ccgcagcaga tcgaaaacct gcggcagggc cgtcctgcgg gacagatgca
     1081 ggaccgcgag tggcctccgc atccgcagcc catggcgcgg cctgaagcgc ggcctcagcc
     1141 accgcggaga taaatcctga ctattccagc ggaagaagct gaatcattac ggatttgctg
     1201 gcttacagga cctgcgaatt cacgtccgga tcggggtgga actggacttc acgcatcgcg
     1261 tgggccactt cacggttctt catgaactgt tcccagacaa agcccgtgcg caggttttcg
     1321 gccatcagga cgctgatgcc ctggtctatg ccgagaatgt ctggagcgta ccagttcgct
     1381 ttgggattga aagcatccac aaagccgtag cgcgtccaga ccttttcacc atatttttcg
     1441 cgcatggtca acaggacgtg cgagcattca gcgggcagaa acggcagcga cccggcgcag
     1501 gcacatggaa ccacggtgcc gtcgagcggg ccaatcgccg gagggccgcc ccatacgcga
     1561 tatccctcgc gtgaatcgga tgcagagatg ccccacaggt cctgatcaat ccaggagaat
     1621 ttcccgccca gagtcatgca ccagagctga tgcacgcgcg tagcggcaat ggaatttgtg
     1681 aagtagttgg tatgcaaatc gcgctgattg cggtagtcgc accaggcgtg cgcgtactgg
     1741 tgaatgaaca gaggcgcgag tccgctgatg tagttgatgc cgccgaactg taccagcggg
     1801 cgttgcaggg cattccatgt ttctgacggg atgggatgcg tgggcgagcc aatcgccagc
     1861 aaatacatgg tcatcagttc ggaatagatg tcccagcggt gtttcaggaa cccaagatcg
     1921 ggcagccatc ccatggagag cgtagtgccg ccattgagca tccattgcca gtcaatgcgg
     1981 cggtagaacg tcgtagccag cgcctcgatg cgcgcattcc cggaaaagta ttgtctgcag
     2041 gtgagtacgc cgcagagcag caatgcagta tcaatagaag aaagctcgct gccgaacatg
     2101 cgctggccgc tttccatgtc aataaagtgg tagagaaagc cgtgttcgtg cgggcagtct
     2161 tccagcagga aagcgagcgt cttttcgacg cgctcttcgc attgcagcct gggcaggtag
     2221 ttgcgtttcg atgcgatgca cagggcggta aggccgaatc cggtggcggc aatgctggcc
     2281 acacgtccca aagaacggcc tgcaacggga gcgcggtcgc gcaccaggcc tgtttttgga
     2341 tgcgcttcat tgtagaagta atcgcaggcg cgaccttcca gatcatcgag caaggcatcg
     2401 gcctgttttg atacctgggt cagcgggcga ttgatgaaga cacccactcc gcgctgatac
     2461 tgttgcgcag cctgttcttc tgtctgcggc tgctggcctg gttttaccgg aggcggctgc
     2521 ggctggttct gcccggcagc aggaaggccg agcgccagaa caccagaggc gccggcattg
     2581 cgcagaagat cgcggcgggt tatacgagaa cgtgacaagc gcaggaaatc tctaacagat
     2641 gaaatgaccg gaaagtttga ttgtattcac gccgcggtat tcgctcctcc tgtgcctgaa
     2701 agaaaatttt tacagatgac gaaagtacag tccagcatgg atattctaag tttctgattt
     2761 ttatgatctc gatacggtct tcaactttga aggatgcaga tgtggtgtgg gagatccttg
     2821 aaccagtcat ccgcgacgga gaaacgtata cacttccgcg cgaccttacc cgcgaacagg
     2881 ccctccagta ttggtattcg cctcaacacg aggtgttcgt tgccgggaaa gatggcgagg
     2941 tcctcggaac atactatctg cgcgccaacc agcagggcgg aggcagccat gtcgccaatt
     3001 gcggatatat gaccgcttct cgcgcaagag gccagggtgt ggccagcgc
//
LOCUS       DASRBV010000381         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_341109, whole genome shotgun sequence.
ACCESSION   DASRBV010000381 DASRBV010000000
VERSION     DASRBV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341109"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFA02_13900"
     CDS             <1..475
                     /locus_tag="VFA02_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10993.1"
                     /translation="RLLPDAFVYKYEGMVEGPNGPCYKLSFQPNPNFNPPDHESQVFA
                     GMAGELWIDKAQERMVRLDAHLIADVNFGWGILGKLYKGGSILVEQADVGEHHWETTH
                     MKLSLTGKALMIKPLSFQTTEDASHFEPVPPDMGYQKAIEMLLSNNTSPQVAQRR"
     gene            630..1331
                     /locus_tag="VFA02_13905"
     CDS             630..1331
                     /locus_tag="VFA02_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZU10994.1"
                     /translation="MSQQEIVLKIFEFVILLFALSLHEAAHAWMASRLGDPTARMLGR
                     VTLNPIKHIDPLGTVLIPLAMLFFSHGFLFGWAKPTPVTTRNFRKIVRDDILTTLAGP
                     LSNVLAAVVSLVLLAVVAKATPTGNLMIKELVVLGGPTQELMAASPVVAPLVVLLYLS
                     ILLNLFLAAFNLIPLPPLDGSHIIRHLLPYSALRVYDSLGMISLVLILFFGGPVVNAI
                     ISPALYVFNQVLLHL"
     gene            1376..2425
                     /gene="trpS"
                     /locus_tag="VFA02_13910"
     CDS             1376..2425
                     /gene="trpS"
                     /locus_tag="VFA02_13910"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZU10995.1"
                     /translation="MSNSSSALNKRVLSGMRPTGKLHLGNYMGALRNWVRLQNEYSCY
                     FFIADWHALTTDYADPSQVKQNTLDVALDFLAAGLDPEKCVIFVQSHVKQHAELHLLF
                     SMITPLGWLERVPTYKEQQENISGKDLSTYGFLGYPLLQAADILVYQPDYVPVGQDQV
                     AHVELTREVARRFNQFYKAQDTGAEILPEPQVLLTPSPKLPGTDGRKMSKSYGNTILL
                     SDPEPVVRQKIKPMVTDPARIRRTDPGNPDVCPVGDLHKVFSGQETITKVYAGCTTAG
                     IGCIECKSWVADSIVRELAPIQERRSKYEQNPQLVQDILHAGSKKAATRAEQTMQEVR
                     TAMGLAENLLDVAVQ"
     gene            2422..>3207
                     /locus_tag="VFA02_13915"
     CDS             2422..>3207
                     /locus_tag="VFA02_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZU10996.1"
                     /translation="MTGKLKPENQDAPSFPSERTQVAQDAPPPQKEKEKEDSAFPFSV
                     TVGKVYDGPLDLLLDLIRKQDIDIYDIPIAKITAQFLSYVEHLKATDVDAAGEFIYMA
                     ALLIHIKSKMLLPRSPVETAEGEPEDPRRELVERLLEHERFKNAAQMLSQKQIIEEST
                     WSNPTLKDFRDDEGTEPEIAADTLDLARVFREILDRMRQRPVLDVEEDTVTVGQMIDY
                     VRRRLITEDKPVSLRKLLANAKSERTLIALFLALLELVRLQAIL"
BASE COUNT          717 a          984 c          857 g          649 t
ORIGIN      
        1 tcgtctgctg cccgacgcct ttgtttataa atacgaaggc atggtcgagg gcccgaatgg
       61 cccgtgctat aagctgagtt ttcagccgaa cccgaatttt aatccgcccg accacgaatc
      121 tcaggtattt gccggaatgg caggggagct ttggatagac aaggcccagg agcgcatggt
      181 gcggctcgac gcgcacctga tagccgacgt aaatttcgga tggggcatcc tcggcaagct
      241 gtataaagga ggcagtatcc tggtggaaca ggccgacgta ggcgagcacc actgggaaac
      301 cacacacatg aagctgagtc ttaccggaaa ggccctgatg atcaaacccc tcagcttcca
      361 gaccacagaa gacgccagcc acttcgagcc tgtcccgcca gacatgggct accagaaagc
      421 gatcgaaatg ctcctgagca acaacaccag cccgcaggtg gcgcaacgaa ggtaggggcc
      481 atgccttctt gaatggtcat cctgagcaaa gcgccgttga tccccaccgg agttgcatag
      541 aacctgcatg ttgcgatgcc ggatcgagca acggacttcc tgaaagcatc cctccacccc
      601 gctctccctg ataccatcaa gactgttgca tgagtcagca agagattgtt ctcaagattt
      661 tcgaatttgt tattctgctc tttgcgttga gcctgcatga ggccgcccat gcctggatgg
      721 cctctcgtct cggcgacccg acggcgcgca tgctggggcg cgtcaccctg aatcccataa
      781 agcatattga cccgctgggc acagtactca ttccgctggc catgctcttt ttttctcatg
      841 gcttcctctt cggatgggcc aaacccaccc cggtcacgac gcgcaatttc aggaaaatcg
      901 tgcgtgatga cattctcacc accctcgccg gaccattgag caacgtgctg gccgcggtcg
      961 tctcgttggt tttgctggcc gtagtcgcaa aagccacacc cactggaaac ctgatgatta
     1021 aggaactggt agtgctcggc ggccccacgc aggaactgat ggccgccagc cctgtcgtcg
     1081 cgccgctggt cgttctgctc tatctcagca ttctgctgaa cttgtttctg gcggcattta
     1141 acctgattcc cctgcctccg ctcgatggct cgcacatcat ccggcatctg ctgccttaca
     1201 gcgcgctgcg cgtgtatgac tcgctgggaa tgatcagcct ggtgctgatt ctcttttttg
     1261 gcggcccggt ggtgaatgcc ataatcagcc ctgcgcttta tgtattcaac caggtgctgc
     1321 tgcatctgtg actcccgtac aataagacaa gcaatttcct tacgcgaagg acacggtgtc
     1381 gaattcctca tctgctctga acaagcgcgt gctcagcggc atgcgtccaa ccggaaagct
     1441 gcatctgggc aactacatgg gagcgctcag gaactgggtg cgcctccaga acgaatacag
     1501 ttgctacttc ttcatcgccg actggcacgc cctcacgacc gattacgctg atccatcgca
     1561 ggtgaagcag aacacgctgg atgtggcgct cgattttctt gccgccggac ttgaccccga
     1621 aaagtgcgtc atctttgtgc agagccacgt aaaacaacat gccgagctgc atttgctttt
     1681 ctccatgatt acgccgctgg gctggctgga gcgcgttcct acctataaag agcagcagga
     1741 aaacataagc ggcaaagacc tttcgaccta tgggttcctc ggctatccgc tgctgcaagc
     1801 ggcagacatt ctggtttacc agccagatta cgtccccgta ggccaggacc aggtggcaca
     1861 cgtggaactc acgcgcgagg tggcgcgccg gtttaaccag ttttataaag cgcaggatac
     1921 aggtgcagag attctgcctg agccgcaggt gcttctgacg ccatccccga agctccccgg
     1981 taccgacgga cgcaagatgt ccaagagcta cgggaacacg attctgcttt ccgatcccga
     2041 acctgtcgtg cgccagaaga tcaagcccat ggtcactgac ccggcgcgta ttcggcgcac
     2101 cgaccccggc aaccccgacg tatgcccggt gggcgacctg cacaaggtct tttccggtca
     2161 ggaaaccata acgaaggtct atgcaggctg caccacggcc ggcatcggct gcatcgaatg
     2221 caaaagctgg gtggcagatt ccatcgtgcg cgagcttgct cccattcagg agcggcgcag
     2281 caaatacgag cagaatccgc agttggtgca ggacattctg catgcaggct caaaaaaggc
     2341 cgccacccgc gccgaacaga ctatgcagga agtccgcaca gcgatgggac tggcagagaa
     2401 tctcctggat gtggcagtgc aatgactggc aagttgaaac ccgaaaacca ggatgccccg
     2461 tcctttcctt cggaaaggac gcaggttgcg caggatgccc ctccacccca aaaagaaaag
     2521 gagaaggagg acagtgcctt ccccttctcc gtcactgtcg gcaaggtcta cgacggcccg
     2581 ctcgacctgc tgctcgacct catccgtaag caggacatag acatctacga catccccatc
     2641 gccaaaatca cggcgcagtt cctctcctat gtcgagcacc tgaaggccac cgatgtagac
     2701 gcagcgggcg agttcatcta catggccgcc ctgctcatcc acatcaaaag caaaatgctg
     2761 ctgccgcgct ctcctgtgga aacagccgaa ggcgagccgg aagacccgcg gcgcgaactg
     2821 gtcgaacgtc tgcttgaaca cgagcgcttt aagaacgccg cgcagatgct ttcccagaag
     2881 cagatcatcg aagagtccac ctggtcgaac ccgaccctca aagatttccg cgatgacgaa
     2941 ggcacggagc cggagattgc cgccgacaca ctcgaccttg cgcgtgtctt tcgcgaaatc
     3001 ctcgaccgca tgcgccagcg tcctgtcctc gatgtagagg aagacaccgt caccgtcggc
     3061 cagatgattg actacgtccg ccgcagattg attacagaag acaagcccgt ctcattgcgc
     3121 aagctgttgg ccaacgcaaa atccgagcgc acgctcatcg cgctcttcct cgccctgctg
     3181 gaactggtgc gcttgcaggc cattctg
//
LOCUS       DASRBV010000382        10986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_390635, whole genome shotgun sequence.
ACCESSION   DASRBV010000382 DASRBV010000000
VERSION     DASRBV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390635"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFA02_13920"
     CDS             <1..357
                     /locus_tag="VFA02_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU10997.1"
                     /translation="QMEDRPILSVSVPFAFTVENTRMPAGEYIVSVIYRDHLWKLSTV
                     DHKANVLLRVSPDFGNGTLPDTQLIFLHYPTDYVLSQISDRDMDVIATVYAGKRARQL
                     ASFSTSELVMIKGKAF"
     gene            685..3099
                     /locus_tag="VFA02_13925"
     CDS             685..3099
                     /locus_tag="VFA02_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZU10998.1"
                     /translation="MAVLAPQYRWIEGQGNAFGAPGLEPRWTSSAKDAVGTAYSSSSR
                     AWYTVSHGILNEVYYPTIDRPQIRDMELLVTDGETFFHEERRDLKHDFEYMDADALAV
                     RVCNRDCEGRYRITKEIINDPHYPVVLTHVRIEGDPGLLPRLKVYVLLSPHIEGGGMG
                     NNARVADVAGERVLIAWKNHTSLAVAADCGFSRASCGYVGASDGWQDLKQNFQMDWEF
                     GSALDGNVAVMGEVYLCAGKSESIEFTVALGFGDGHHAALAVTMAALAKPFDEHLKRF
                     IEQWHRAASPRELAASSTDGGRLMQISHNIILAHEDKTYGGAFIASASIPWGYAKSDD
                     DLGGYHLVWTRDMVQSATALLACGRIETARRALVYLACTQKPDGGFAQNFWIDGTPYW
                     TGVQLDEVAFPILLAWRLWKLNGLGKFDVVPFVERAAGFLVRHAPVTQQERWEEAAGY
                     SPSTLAAVISALVCAADIARAHDSVELGQFLEEHADWLQSHLEDWTVTNNGVLHPEVK
                     RHYMRIRPPECGEPYAHEECGTEMVRIANGKPGEKNEYEAREIIDAGFLELVRYGVRR
                     ADDPLIVDSLKVVDHVLKVETPKGPCWYRYNHDGYGQREDGGPFLGHGKGRPWPLLVG
                     ERAHYELAAGSDVKPLIATMERYASEGGMLPEQIWEELDRYGLVCGGPAGSAMPLVWA
                     HAEYLKLLRSVTDGRVFDRISIVEERYREKRESHIEVFKLRRPIRQITAGKTLRITAQ
                     ERFKVLWSTDGWKTQQSGMSSTVGYAGSYADIPTDKNLSGTVSFTLFWTERQQWEGRN
                     FEVKLE"
     gene            3333..5351
                     /gene="tkt"
                     /locus_tag="VFA02_13930"
     CDS             3333..5351
                     /gene="tkt"
                     /locus_tag="VFA02_13930"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZU10999.1"
                     /translation="MSDIDQLSINALRFLAVDAVEKAKSGHPGAPLGDAPMAYLLFHK
                     YMRHNPANSHWANRDRFVLSNGHASALLYGVLHLSGYKVSIEDLQQFRQWGSHTPGHP
                     ERGDTDGVEVTTGPLGQGFAMGVGLAIAEKHLSAVYNRPGFDIVDHYTYGICSDGDLM
                     EGISHETASLAGTLGLGKLIYLYDDNLISLDGPTDLSYTEDVLKRFEAYHWHVQRVND
                     GNDLDALSKAIEAAKAVKDRPSLIAVRTIIGYGSPLAGTNKVHGEAMGPDNTKKTKEA
                     LGWPADQTFYVPEEAKKNWLTIVDRGKKAEAEWNALFAEYRKQYPELATEFERTQAGK
                     LKEGWEKTIPVFPTDKPVATRNAGNVVMNAFAKEVPELIGGAADLTASTKTIVKDGGN
                     FHLDAKGVNIWYGVREFGMCAIVNGMAAHGGVIPYGSTFFVFTDYAKPALRMAAIMRV
                     HSIFVFTHDSIALGEDGPTHQPIEHLMALRAVPHLTDFRPADANETAAAWRLALERNS
                     PSWMALSRQDIPVLDAAQHNIYEGVKRGAYIVEKGGDSPDLLIVATGAELWPSLEAAA
                     ALKKDGITARVVSMPSWKLFEEQSEEYKTSIFPDHLPKLAVEAGSPIGWWKYVGRHGD
                     VIGLERFGASAPGKTVLEKLGFNAANIASRGKALVERVRKTEAVMAGK"
     gene            5354..5842
                     /gene="rpiB"
                     /locus_tag="VFA02_13935"
     CDS             5354..5842
                     /gene="rpiB"
                     /locus_tag="VFA02_13935"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZU11000.1"
                     /translation="MKLVIAADHAGFPLKEEVRGYLERLGHEVIDLGAYNTEPSDYPD
                     FAEAVGKALAAGRAERGILICGSGVGVCVAANKMPGIRAAMCHDTYSAHQGVEHDDMN
                     VLVLGARIIGPALAFDLVSSYLNARFQKQEERFVRRLNKVKAIEARNMPEAAKLESHT
                     AK"
     gene            5887..6513
                     /locus_tag="VFA02_13940"
     CDS             5887..6513
                     /locus_tag="VFA02_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZU11001.1"
                     /translation="MATISTILWDVGGVLLTNGWDHHERAAVLSHFGIDRDAFEQRHP
                     EANDAWEKGLIPVEEYLARTVFYEQRNFTPQNFVDAMKAQSKVLDDSALGILGELAAS
                     DSVMLGMLNNEARELNDFRIEKFRLTDYFDVFLSSCYVGLRKPDARMFRLACDVLQRK
                     PGEIIFVDDRSGNVQAAKDAGMLGIVYEGAEKLKSELAEAGVLQASLG"
     gene            6687..7601
                     /gene="gnd"
                     /locus_tag="VFA02_13945"
     CDS             6687..7601
                     /gene="gnd"
                     /locus_tag="VFA02_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZU11002.1"
                     /translation="MELGIIGLGKMGGNMAERLREAGHKIVGFDFNADAVKRLTDAGS
                     VGVSSLEDLVKNLSAPRAIWIMVPSGDPVDQTIAKLEPLMQKGDTFIDGGNSNYKDSQ
                     RRYAALKAKGFHFVDVGTSGGVWGLKEGYSMMIGGDKEPVERLRPIFEALAPAKDKGW
                     GHVGPAGAGHFVKMIHNGIEYGMMQAYAEGFSIMQHKAEMDLDLTQIAEIWRYGSVVR
                     SWLLDLTAEALKKNPTLDGLEAYVADSGEGRWTVFEAIDLNVSAPVITESLIRRIRSR
                     EENNFTDRMLAIMRNAFGGHAVKTEKQG"
     gene            7605..9149
                     /gene="zwf"
                     /locus_tag="VFA02_13950"
     CDS             7605..9149
                     /gene="zwf"
                     /locus_tag="VFA02_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZU11003.1"
                     /translation="MATATEVRVAPEDVKAAAQRSERIPDPAVVVIFGASGDLTKRKL
                     LPALFHLEQQGLLPAEFAIVGVARRDLKSTFAADMKDGILKYGGGKDGDGTIDSFIGK
                     VDYHAMNFDDDAGYIELKSLLEKIDEERGTQGNRLFYLAVAPEYFADIINRLGEHGMA
                     HPEKGYARVIIEKPFGHDLKSAKELNDDVNRVFDEDQIFRIDHYLGKETVQNILVFRF
                     ANGIFEPIWNRNYIDHVQITAAESIGIEGRGPFYEKAGALRDVVQNHMMELLSFVAME
                     PPVSFEANAVRGEKVKVWRAVKPISIENTVRGQYALGMVDGQQVQGYREEDRVDPNST
                     TETFAALKLEIENWRWAGVPFYLRAGKRLAKRVTEIMIQFKQPPLLLFDRSGGGPCSE
                     IQPNILTIRIQPDEGISLRFGAKVPGPDTNVCPVVMDFQYATAFGVNSANGYERLLLD
                     AMLGDQTLFAHRDGVEATWALFTPVLEAWAKSKPKDFPNYVSGSWGPKAADGLIGRDG
                     RKWHRI"
     gene            9261..10058
                     /gene="pgl"
                     /locus_tag="VFA02_13955"
     CDS             9261..10058
                     /gene="pgl"
                     /locus_tag="VFA02_13955"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZU11004.1"
                     /translation="MPRKIVVEYRVYDGPDALAHAAAEHFLETAQQAIAHRGKARVAI
                     SGGSTPKRTFELLADPQEKFLKAMPWDKLELYWVDERTVPPTDKDSNYRMTRESLLEK
                     VPLKPEQVFRMEGELDPEEAAARYESLIRNNFRLEGAEVPQFDAVQLGMGDDGHTASL
                     FPHTQAIHELGRIVVANHVPQKDTWRITLTWPVLIEASEVFFLIGGKDKADPLHRVLQ
                     GPYDPETLPSQLIRPKSGKLLLLLDKDAAAQLPAPGADGRGRLEIER"
     gene            10055..>10986
                     /gene="glk"
                     /locus_tag="VFA02_13960"
     CDS             10055..>10986
                     /gene="glk"
                     /locus_tag="VFA02_13960"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZU11005.1"
                     /translation="MILAGDVGGTKVHLALYGFEKGELTHVRDEKFPAQEYAGLEVIV
                     RRFLEESGEPEVTAACFGVPGPVRQGRLKLTNLPWILDSRELSKNLDIQHLFLINDLE
                     ANGYGIPELHPEQIYVLSEGDPAAVGNRGLVSAGTGLGEAILVWDGRTHVPIASEGGH
                     CDFAPRNELEIELLRYLQRRLNGRVSYERVVSGIGLQNIYSFLRDEKKMDEPAWLRER
                     MKEEDPNAVIGQLGESGENELCVRTLDIFASAYGAEAGNLALKALAIGGMYLGGGIAP
                     KILKKMKDGAFMKAFTDKGRLSDLLVHTPVRIIL"
BASE COUNT         2465 a         2669 c         3510 g         2342 t
ORIGIN      
        1 cagatggagg accggcccat tctttctgtt tcggttccat ttgcctttac ggtggaaaat
       61 acgcgaatgc cagccggcga atacatcgtt tcagtcatct accgcgacca cctttggaaa
      121 ctcagcacgg tagaccacaa agccaatgta ctgttgcgtg tcagtccgga cttcggtaat
      181 gggacgcttc ccgacacgca gctgattttt ctccattacc cgacagatta cgttttgtcc
      241 cagatcagcg accgggatat ggatgtgatt gcgacagtct acgcggggaa acgggccagg
      301 caactggcca gtttcagcac gtcagaactg gtaatgatca agggcaaggc tttttgattg
      361 cggatctgtt tcggtgagct atcagccagc ttttcctata gcggtttttc gcataactat
      421 acccaataag tccggactgt ttttgaggcc ctttagccag gcgaaggaga tgaagaatcg
      481 ccagcgttag ccgttaccca acctcctttc gggcgtgctt aaaaacacgc cctacttttt
      541 tgaataaaac cccctcactt ttaaattcgt tcggagtgga aatccacaac ataaaaattc
      601 ttgtatcgag caaatttata cttccatcca acgttttgga gtacattgca atttctgttc
      661 ttttacgaag ggagtggtct ctctatggct gtgctggcgc cgcagtaccg gtggatcgaa
      721 gggcagggaa atgcatttgg agcgccggga ttggagccgc gctggacctc aagcgccaaa
      781 gatgcggtcg ggacggcgta ctcttcttcg agccgcgcct ggtacacggt ttcacacggc
      841 attctgaacg aagtctatta ccccaccatt gaccgcccgc agatccgtga tatggaactc
      901 cttgtcaccg atggcgagac gttttttcac gaagagcgcc gcgacctgaa gcatgatttc
      961 gagtacatgg atgcggatgc gctggcggta cgagtctgca atcgtgactg cgagggccgt
     1021 tacagaatca caaaagaaat catcaatgat ccgcattatc cggtggtgct gacccatgtc
     1081 cggattgagg gcgatcccgg cctgttgccg cggctgaagg tgtatgtgct gctttcgccg
     1141 catatcgaag gcggaggcat gggtaacaac gcgcgcgttg cggatgtggc gggggaacgc
     1201 gtcctgattg cgtggaagaa ccatacatct ctcgccgttg cggccgattg tggattttcc
     1261 cgagcgagct gcggttatgt tggcgcgagc gatggctggc aggacctgaa gcagaatttc
     1321 cagatggatt gggagttcgg atccgcactc gacggaaatg ttgcagtgat gggcgaggtg
     1381 tatctttgtg ccggaaaatc tgaatccata gagttcacgg tggcgctggg gtttggcgat
     1441 gggcatcatg cggcccttgc cgtcaccatg gctgcgctcg ccaagccctt tgacgaacac
     1501 ctgaagcggt tcatcgagca gtggcaccgg gcggcgagtc cgcgggagtt ggcggccagc
     1561 tcaaccgatg gcggcaggct gatgcagatc agccacaaca ttattctcgc gcatgaggac
     1621 aagacctacg gcggcgcgtt tattgcgtcg gcctcgattc cctggggata tgcaaagagc
     1681 gatgacgatt tgggtggata ccacctggtc tggacgcgcg acatggtgca gagcgcgacc
     1741 gctttgctgg cctgcgggcg cattgagacg gcccggcgcg cgcttgtgta tctggcctgc
     1801 acgcaaaaac cagacggcgg gttcgcgcag aatttttgga ttgacggcac tccctactgg
     1861 actggcgtgc aactggacga ggtggcattc cccatccttc tggcgtggcg attgtggaag
     1921 ctgaatgggt tgggcaagtt tgatgtagtt ccatttgtgg agcgcgcggc tggatttttg
     1981 gtacggcatg ctccggtgac acagcaggag cgatgggaag aagcagcagg ctactcgcct
     2041 tcgacgctgg ccgcggtgat ttcagccctg gtgtgcgcgg ctgacattgc acgggcgcat
     2101 gattcagtgg agctggggca atttctggaa gagcacgccg attggcttca aagtcatctg
     2161 gaggactgga cggttaccaa caatggtgta ctgcatccgg aggtgaagcg gcactatatg
     2221 cgcattcgtc cgccggaatg tggggagccg tatgcgcatg aagagtgcgg cacggagatg
     2281 gtgcgtatcg cgaatggcaa accgggcgag aaaaatgagt acgaggcgcg cgaaatcatt
     2341 gatgcgggct ttctggagct ggtgcggtat ggggtgcgaa gggcagacga tccgttaatc
     2401 gtggattcat taaaagttgt agaccatgtg ctaaaggtag agactccgaa gggaccgtgc
     2461 tggtaccgct acaaccatga tggatatggg cagcgggaag acggcgggcc gtttcttggg
     2521 catggaaagg ggcgtccatg gccgctactt gtgggcgagc gcgcgcacta tgaattggca
     2581 gcagggagtg acgtgaagcc gctgattgcg acgatggagc ggtatgcgtc agaaggtgga
     2641 atgcttccgg agcagatatg ggaggagctg gaccggtatg ggctggtgtg cgggggtccc
     2701 gcggggtcag cgatgccact ggtgtgggcg catgcggaat atctgaagtt attgcgctcg
     2761 gtcaccgacg ggcgggtgtt cgaccgcatc tctatagtag aggaacggta cagggagaaa
     2821 cgcgaaagtc atattgaagt tttcaagctg cggcggccaa ttcggcagat tactgccggc
     2881 aaaacgctca ggattacagc acaggagcgg ttcaaagtgc tgtggagcac ggatgggtgg
     2941 aagacacagc agtccggaat gagttccact gtgggctatg cggggtctta tgcggacatt
     3001 ccgacggaca agaatctgag tgggacggtt tcgtttacgt tgttctggac cgaacggcag
     3061 cagtgggaag gaaggaattt tgaggtgaag ctggagtagt cagattgtgc ggacccattc
     3121 ccgcaaaacc ggcgcgaaag gatggatcac ccggtattct gctgaggcgg aaccgttgtc
     3181 ataagtctgg gtgatatccg ggataaaatt cgctgatttt tcgttttgcg cggcatgggt
     3241 gagaataggg agaaggggcg gaaacttctg ctgcgccgat tgttattgtc caggtcataa
     3301 aaagaaattt tctgggagcg tgaataaagg ttatgagcga tattgatcaa ctttccatca
     3361 atgcactccg ttttctggcg gtggatgccg tagagaaggc gaagagcggg catccgggcg
     3421 cgccgctggg cgatgcaccg atggcgtatc tgctgttcca taagtacatg cggcacaatc
     3481 cggcgaactc gcactgggca aaccgcgacc gctttgtgct ctcgaacgga cacgcttccg
     3541 cgctgctcta tggggtgctg cacctttccg gatacaaagt cagcattgag gacctgcagc
     3601 agttccgtca gtggggatcg catacgccgg ggcacccgga gcgcggtgat acagatggtg
     3661 tggaagtcac gactgggccg ctggggcagg gttttgccat gggggttggt ctggccattg
     3721 cagaaaaaca cctgtctgcc gtctacaacc ggcctggctt cgatattgtt gaccactaca
     3781 cctacggaat ctgttccgac ggcgacctga tggaaggcat ctcgcacgag acggcctcgc
     3841 tggctgggac gctgggcctg ggcaagctga tttacctcta cgacgacaac cttatctctc
     3901 tcgacggtcc tacggacctc tcctatacag aagacgttct gaagcgcttt gaggcgtacc
     3961 actggcatgt acagcgcgtg aacgacggca atgacctcga cgcgctttcc aaagccattg
     4021 aagcggcaaa ggcagtcaag gacaggccat cgctgattgc cgtgcgcacc ataatcggtt
     4081 acggaagtcc gctggcgggg acgaacaagg tccacggcga ggcgatgggg ccagacaaca
     4141 cgaagaagac caaagaggcg ctgggctggc ccgcagacca gacattctat gttcctgaag
     4201 aggcaaagaa gaactggctg acgattgtgg accgcggtaa aaaggccgag gcggaatgga
     4261 acgcgctctt tgccgagtac cggaagcagt atccggagct ggccacggaa tttgaacgca
     4321 cgcaggcggg caagctgaag gaaggctggg agaagaccat tcctgttttc ccaaccgata
     4381 agcctgtggc gacgcgcaac gccgggaatg tggtcatgaa tgcctttgcg aaggaggtgc
     4441 cggaactgat tggcggcgcg gcagacctga cggcctcgac caagacgatt gtgaaggacg
     4501 gcggtaactt ccatctggat gcgaagggcg tgaacatctg gtatggcgtg cgcgagttcg
     4561 gaatgtgcgc catcgtaaac ggaatggcgg cgcatggcgg ggtaattccg tatggttcga
     4621 ccttcttcgt cttcacagac tacgcgaagc ctgctctgcg catggcggcg atcatgcgcg
     4681 tgcattcgat attcgtgttc acgcatgatt cgattgcgct gggcgaggat gggccgacgc
     4741 accagccgat tgagcatttg atggcgctgc gggcggtgcc gcatctgacg gattttcgtc
     4801 cggcagacgc gaacgaaaca gcagcggcat ggcgtctggc gctggagcgt aacagtccat
     4861 catggatggc gctttcgcgg caggacatcc cagtgctcga tgcagcacaa cacaacattt
     4921 atgaaggtgt gaagcgtggc gcatacatcg tagagaaggg tggcgattcg cctgatctgc
     4981 tgattgtagc gacgggcgcc gaactgtggc cttcgctgga agctgcggct gccctgaaga
     5041 aagacggcat tacggcgcgt gttgtctcca tgccaagctg gaagcttttt gaagagcaga
     5101 gcgaagagta caaaacttcc atcttccctg accatctgcc aaagctggcc gtcgaagccg
     5161 gtagcccgat tggctggtgg aagtatgtgg gccgccacgg agacgtgatt ggtctggaac
     5221 gctttggcgc atcggccccg ggtaagactg ttctggagaa gctgggcttc aacgcagcga
     5281 acattgccag ccgtggcaag gcgctggtgg aacgcgtccg caagaccgaa gctgtcatgg
     5341 cggggaaata gccatgaagc tggtgattgc agccgatcac gcgggctttc ctcttaaaga
     5401 ggaggtccgc ggctacctgg agcgtctggg ccatgaggtc attgacctgg gcgcttacaa
     5461 cactgagccg tccgactatc ctgattttgc tgaagctgtg ggcaaggcgc tggcagcagg
     5521 gcgggccgaa cgcggcatcc tgatctgcgg cagcggcgta ggagtttgcg ttgcagccaa
     5581 caagatgccg ggcatccgcg cggccatgtg ccatgacaca tactccgcgc atcaaggcgt
     5641 cgagcacgat gatatgaatg tcctggtgct gggtgcgaga atcattggtc cggcgctggc
     5701 gttcgatctg gtctcgtctt acctgaacgc gcgtttccag aaacaggaag agcgttttgt
     5761 gcgcaggctg aacaaggtaa aagccatcga ggcccgcaat atgcctgagg cggcgaaact
     5821 ggaaagccac acggcgaaat aatgattctg tcgaaataac aaaagcactg ctgcgcagga
     5881 gcatggatgg caaccataag cacgattttg tgggatgttg gcggggtcct gctgaccaat
     5941 ggctgggacc atcacgagcg cgcggcggtg ctttcgcatt ttggaataga ccgcgatgct
     6001 ttcgagcagc gccatcctga agcgaatgac gcctgggaaa aagggctcat ccccgtcgaa
     6061 gagtatctgg cgcgaacagt cttttacgag cagcgcaatt ttactccgca gaactttgtg
     6121 gatgcgatga aagcgcaatc gaaggtgctg gatgacagcg ccctcggaat cctcggagaa
     6181 ctggcggcat cagactcagt gatgctggga atgctgaaca acgaagcgcg cgagctgaat
     6241 gatttccgga ttgagaagtt ccgtctcacg gattatttcg atgtatttct cagctcgtgc
     6301 tatgtgggcc ttcgcaaacc ggatgcgcgg atgttccggc tggcctgcga tgtattacag
     6361 cgcaaaccgg gcgagataat ttttgtggat gaccgctccg gaaatgtgca ggcggcgaaa
     6421 gatgctggga tgcttggcat cgtgtatgaa ggcgccgaga agctgaagtc ggagttggcg
     6481 gaagcaggtg ttcttcaggc gagtttgggt tgattccagg tagaagtgcc ggtctttaga
     6541 ccggtgaaag gctgaaatca acggctttag ccgcggttac aggaataaag gttttagagg
     6601 gtgcttcgat atttgtggtc tccaggccca tcgtgcaaca acggggccca tagggagtat
     6661 cggaaacaaa aaactttgga ggatgtatgg aacttggcat cattggtcta ggaaagatgg
     6721 gcggcaatat ggcggagcgt ctgcgcgaag ccggacacaa gattgtcggc tttgacttta
     6781 atgcggatgc ggtaaagagg ctgacagatg ccggttctgt gggcgtttct tcgcttgaag
     6841 acctggtgaa aaacctgtct gcgccgcgcg ccatctggat catggtcccc tcgggcgatc
     6901 ccgtagacca gaccatcgcc aaacttgagc cactgatgca gaagggtgac acatttattg
     6961 acggcggcaa ctccaattac aaggattcac agcgccgtta cgccgcgctg aaggccaagg
     7021 gctttcattt tgtagacgta ggcacctcgg gcggtgtgtg gggcctgaaa gagggctaca
     7081 gcatgatgat cggcggcgac aaggagccgg tcgagcggct gcgccccatc tttgaagcgc
     7141 tggccccggc aaaagacaag ggttggggac acgtcggccc cgccggcgct ggtcactttg
     7201 tcaagatgat ccacaatggt attgagtatg gaatgatgca ggcgtatgca gaaggttttt
     7261 ccatcatgca gcacaaggca gaaatggatc tggacctgac gcagattgca gaaatctggc
     7321 gctacggcag cgtagtgcgg tcgtggctgc tcgaccttac agcagaagcg ctcaaaaaga
     7381 atcccacgct cgatggtctg gaggcctatg ttgcagactc gggcgaaggc cgctggacgg
     7441 tattcgaggc cattgatctg aacgtctctg cgccggtgat taccgagtcg ctgatccggc
     7501 gcattcgatc gcgcgaagag aacaatttca ccgaccgtat gctggcgatc atgcgcaacg
     7561 cctttggtgg acacgcggtc aagaccgaga aacaggggta agttatggcg acagcaactg
     7621 aagtcagggt tgcacctgag gacgtaaagg cagcagcaca gcgcagcgag cgcattcctg
     7681 atcccgcagt ggtggtcatc tttggggcat cgggcgattt gaccaaacgg aagctgctgc
     7741 ctgcgctgtt ccatctggaa cagcaggggc tgcttccggc agagtttgcg atagtgggcg
     7801 tggcccggcg tgatctgaag tctacttttg ccgctgatat gaaagacggg attctgaaat
     7861 acggcggcgg caaggatgga gacggcacga ttgacagctt cattggcaaa gtggactacc
     7921 acgcaatgaa ctttgatgat gatgcaggct atatcgagct gaagtcgctg ctcgaaaaga
     7981 tagatgaaga gcgtggcacg cagggcaacc ggctctttta tctcgcagtc gcacctgaat
     8041 attttgccga tatcatcaac cggctgggcg agcacggcat ggcgcatccg gagaagggat
     8101 atgcgcgcgt cattattgaa aagccctttg ggcacgatct gaagtcggcg aaagagctga
     8161 atgatgatgt aaaccgtgtg tttgacgagg accagatctt ccgcattgat cactatctgg
     8221 gcaaggaaac ggtacagaac atcctggtct tccgttttgc caatgggatt tttgagccga
     8281 tctggaaccg caattacatt gatcatgtac agatcacggc ggcggaaagc attgggattg
     8341 aaggccgtgg accgttttat gaaaaggccg gtgcgctgcg cgacgtcgtc cagaaccaca
     8401 tgatggagct gctttcattc gtggcgatgg agcctccggt ttcttttgag gccaatgcag
     8461 tgcgcggtga aaaagtaaaa gtatggcgcg ccgtcaagcc gatttccatt gaaaataccg
     8521 tgcgcggcca gtatgcgctg ggtatggtgg atggccagca ggtgcaaggg taccgggaag
     8581 aggaccgggt ggacccgaat tccacgacgg aaacttttgc tgcgctgaag ctggaaattg
     8641 aaaactggcg gtgggccggg gtcccgtttt atctgcgagc gggcaagcgc ctggccaaac
     8701 gtgtgacgga gatcatgatc cagttcaagc aacctcctct gttgctgttt gaccgctcag
     8761 gtggagggcc gtgcagtgaa attcagccca atatcctgac cattcgcatt cagccggatg
     8821 aaggcatttc gctgcgcttt ggcgcaaagg tgcccgggcc ggacacaaat gtttgcccgg
     8881 tggtgatgga cttccagtat gcgacggcgt ttggcgtgaa ttctgcaaac gggtatgagc
     8941 gtctgctgct cgatgccatg cttggcgacc agacgctctt tgcccatcgc gatggagtgg
     9001 aagcgacctg ggcgttgttt actccggtgc tggaggcatg ggcaaaatcg aagccgaagg
     9061 actttccgaa ttatgtaagt ggtagctggg ggccgaaggc cgcggatgga ttgattggac
     9121 gcgacggaag gaagtggcac cggatttaag gaaagccagg tacctggtta tcgtcattct
     9181 gaagaatatg ggcgattttg gctgaaattt ttcgttgctg tgctcctcgg aacgacatac
     9241 cgacaatttt cagaatacag atgccaagaa aaattgtagt tgaatatcgc gtatatgatg
     9301 ggccggatgc tttggcccat gcggcggcag agcatttcct tgagacggcg cagcaggcca
     9361 ttgcccatcg gggcaaagcg cgggttgcca tctctggcgg cagtacaccg aagcgcacat
     9421 ttgagctgct tgcggacccg caggagaagt ttctgaaggc catgccgtgg gacaagctcg
     9481 aactctattg ggtggatgag cgcacagtac ctcccacaga caaggacagc aactatcgca
     9541 tgacgcgcga atcgctgctg gaaaaagtgc cgctcaagcc ggagcaggtt tttcgcatgg
     9601 aaggcgaact ggatccggaa gaagctgcgg cgcgatatga gtcgctgatt cgtaacaact
     9661 tccggcttga aggagcggag gtgccgcagt ttgatgccgt ccagcttggc atgggggatg
     9721 acgggcacac tgcatcgctg tttccgcata cacaggccat ccacgagctt gggcgcattg
     9781 tggtggcgaa ccatgttccg cagaaagata cgtggcgcat cacgctgacg tggcctgtgc
     9841 tgattgaggc gagcgaggtc ttctttctta tcggcggcaa ggacaaagcg gacccgctgc
     9901 atcgcgtgtt gcaggggccg tatgatccgg agacattgcc atcgcaattg attcgcccga
     9961 agagcggcaa gctgctactg cttttggaca aggacgcagc agcgcaactg cctgctcctg
    10021 gcgcagatgg acggggaaga ctggagatag aacgatgatt ctggccggag acgtgggcgg
    10081 aacaaaagtc cacctggcgc tgtatggatt tgaaaaaggt gaattgacgc acgtccggga
    10141 cgagaagttt cctgcgcagg aatatgcagg gctggaagtg atcgtgcgca ggttccttga
    10201 agaaagcggc gagccggaag taacggcagc atgtttcggc gtgccgggac cagtccgcca
    10261 gggaaggctg aagcttacca atctgccatg gattctcgac agccgcgaac tctccaaaaa
    10321 cctcgatatt cagcacctgt ttctcatcaa tgatcttgaa gccaatggct atgggattcc
    10381 tgagctgcat ccggagcaga tttatgtgct gagcgagggg gacccggcag cagtcggcaa
    10441 ccgcgggctg gtttctgcgg gtacagggct gggcgaggcg attcttgtgt gggacgggcg
    10501 cacgcatgtg cccatagcat cagaaggcgg acactgtgat tttgctccgc gcaacgagct
    10561 tgagatcgag ttgctgcgtt atcttcagcg cagactgaat ggccgcgtga gctatgagcg
    10621 ggtggtctcc ggaattggct tgcagaacat ttattccttc ctgcgggatg agaaaaagat
    10681 ggacgaacca gcatggctcc gcgagcgcat gaaggaagaa gaccccaacg cggtcatcgg
    10741 ccagttgggc gagagcggcg aaaatgagct gtgcgtcagg acactggaca tctttgcatc
    10801 agcttatggg gcagaggccg ggaacctggc actgaaagcg ctggcgatag gcggaatgta
    10861 tcttggtggc ggaattgctc cgaagatcct gaagaaaatg aaggacggcg ctttcatgaa
    10921 ggcatttacg gacaagggcc gcctgagcga tctgctggtg catactccgg tacgcatcat
    10981 tcttga
//
LOCUS       DASRBV010000383         9562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_341386, whole genome shotgun sequence.
ACCESSION   DASRBV010000383 DASRBV010000000
VERSION     DASRBV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341386"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFA02_13965"
     CDS             complement(<1..716)
                     /locus_tag="VFA02_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11006.1"
                     /translation="MTYLAKRILFAAFAVVFSTALLHADSGDPSVRVARLHAVQGNVS
                     IQPSDEQQWTQAANNYPISTGDRIYSDQNSRAEIQMGNLVARVWQTTDLTMTNLADQL
                     TQMGLAQGSLRVHTFSLNPGEQVEVDTPNGTLMVTQPGDFRVDSYTGDGGTIVTVNSG
                     QLEVTGPGVSQYVGAGQSVQLTGTNPIQLVSQQMPGLDAFDRWSIDRDHHILNARSVQ
                     YVGRGVVGYDDLDDYGSWADT"
     gene            1052..1660
                     /locus_tag="VFA02_13970"
     CDS             1052..1660
                     /locus_tag="VFA02_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11007.1"
                     /translation="MKTLFLSALAASLLAVAGCRIQVNKAKNGEDKTVKIDTPMGGLH
                     VRTNEMSAADVGLPVYPGAQVVTDSKEKDDGKNKSVDVHMGFGKWQLRVKVINYQTSD
                     SQDNVLAFYRKALGRFGDVIECNNNTPVGTPARTHEGLTCSEHAKNSNVHEADDGRSL
                     RAGSPHHQHIVSIDKNSTAGKTQFALIELELPEGAEISDDSQ"
     gene            1734..2402
                     /locus_tag="VFA02_13975"
     CDS             1734..2402
                     /locus_tag="VFA02_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZU11008.1"
                     /translation="MDPVCHRCGNSLSSSEIFCPHCGAPQLRFEPSDEQPVQRPQTVG
                     APFRIIQRVEWRATVQAALMLALPVGLLSSLWDFGILWMLAGGLCTVALYRKRTMNTV
                     NAGIGWRIGMLTGLLSAYCASLIDGLGMVLERYGLHHGARIDQRLAAMTQQMTEQMAR
                     QNPDAAQQIPWFLHFWLSPDGHAAMVLLMSSFAAVMMIAFSAIGGVLGARIFGARHRL
                     STNL"
     gene            2402..3139
                     /locus_tag="VFA02_13980"
     CDS             2402..3139
                     /locus_tag="VFA02_13980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU11009.1"
                     /translation="MQEYNCSTYGDRIAHLYDDLRQFTPEQTQTEVDALAALAKGGPV
                     LELGIGTGRVALPLKEKGLEVHGIDASPKMIEELRRKPGGSQIPVAIGDFSSVPVEGR
                     FSLIFVTFNTFFALTTQEAQVRCFRSVAAHLAPDGLFVIEAFVPDPSRFTRNQNIVVS
                     RVDLDEVRFDVSQHDPLHQRVVAQHVVITQGNTQLFPVQIRYAWPSELDLMAQLAGMK
                     LKDRWSTWQGAPFTSGSTAHVSVYELM"
     gene            3245..4948
                     /locus_tag="VFA02_13985"
     CDS             3245..4948
                     /locus_tag="VFA02_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU11010.1"
                     /translation="MKRFLLSILGLWLLLYGSFTLVRPPLFDGVDATHAEIAREILVT
                     NDWVTFHLNGVPFFEAPPLLPWCIAVSFKIFGLHDWAARLPLAFSALALFLVVFALGR
                     RCFGNSKAGFWSAACLLTASGIFGFMHMLLPESLFCLWLTLSIYFFRRSLEYEQGQPS
                     ITSACGFAICCALGVLSLGLVGIIFPVAIVLIYLGATKDLGHIFRWHPWVGLLVFLVI
                     ALPWHVRAALRNPGFLWAYFVNGHFLRYLGRHTPASEDPVPLWIFWLLLLIFIAPWCI
                     FAIKMLARLRSVRQSTLLLPAIWAGVVMLFFTFSFRQEYYLLPALPPLALLAGGWLAE
                     DEDSPSPAGRITAWVLLIAGTIAAIASAIFAVTFKPLPIGTDVSAFLQEVSRSHRLFF
                     GHFFDLTLRALGAFRVPLTITTAALLVGVTANLCFRLRKRTGLANAFLLGTVVAFLIA
                     AHLALNTLSPAISSQILANAILPEMDSDDVVIVNGAFENASSLVFYLRRQAKILNGHN
                     RQSQIDQTSLDQLWSGQERVFLWTPNDQVPLLPQPTFVIGRSGGKEILSNQPNSRGAE
                     F"
     gene            complement(5062..6021)
                     /gene="cysK"
                     /locus_tag="VFA02_13990"
     CDS             complement(5062..6021)
                     /gene="cysK"
                     /locus_tag="VFA02_13990"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZU11011.1"
                     /translation="MNAPTTLQPVRALRIAENVTELMGQTPMLRLGRIVTPEMADVYA
                     KLEFLNPGGSVKDRAALGMILDAEARGILKPGSTIVEATAGNTGVGLALVGVNRGYKV
                     VLFVPEGYAEEKCMLMRGFGAKVVRTPEAEGMSGAIREALALAKTDPNIFPAMQFDNQ
                     ANPQFHYETTAQELWEQMEGRIDAFVAGVGTGGTFSGVARLLKEKDARILTVAVETEG
                     SVLQGGEPGPHKVEGIGVSFVPKTFDRSVADEIVRVMDAEAFAMVRRLAAEEGVLAGS
                     SAGANVHAALNVAKRLGQGKRIATIVPDSAERYLSKKIFECAD"
     gene            6242..7876
                     /locus_tag="VFA02_13995"
     CDS             6242..7876
                     /locus_tag="VFA02_13995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZU11012.1"
                     /translation="MGSVVFLTAAAFFVYHTTKDLLSARDWEEHSQEVLNTLQLTSQR
                     LDRLDLTSRLYFAEKNRDDLGAAQTVAVVLVAGVSHLTGLVGDNPAQEALAQALQSCT
                     SELKQIADKTSPADVAGQSIVFGQKVLDCRDYVVRMQADENMLLRQRRDESQRGAYRS
                     LSAGAVFLLVSLVTVLVLFGLLVRDAAQQYKAKQEVFDANARLASTVQLMEQRAVEAG
                     LLTATQEELQLCTNVAQAYQVTVRRCSQLLPSASIALLMINNSRQMLELVAASGGLTQ
                     VVEGFSLDACCGLRGGQIRWRKRGQSEIDCEHFVGAPPENYLCMPLTAHGDTLGILYV
                     ECSPDEDMPHIEAKMEALKSIVKVAAVFFGSLNLRARLENQSIRDGLTNLFNRHFMEI
                     SLDREIRRAARSRTELAVLMIDVDHFKHFNDTFGHEAGDYVLREVAEVFRNTVRVEDI
                     ICRYGGEEFVIIFPETGAESAKTRAEEIRRQIQRLQLRFRGENLRETTVSIGIAVYPE
                     AGNSLVELLRNADRALYAAKNRGRNCVVTSLTEVTA"
     gene            complement(7881..9101)
                     /locus_tag="VFA02_14000"
     CDS             complement(7881..9101)
                     /locus_tag="VFA02_14000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU11013.1"
                     /translation="MRGESRLTGDCLSAQPHIAETRAAVLDPPAATPPPSSKPAKPVS
                     KKISKPKLPALSGLRAFAALNIVFFHFSNPKWFGPFAPIVDNGYTSVSFFLLMSGFIL
                     AYNYSERAQHGQLKATHFWMARLSRLYPVYLFALLVSIGVLIEEWHVRTRGEFFLGAT
                     LTPLLLQGWIPKLATFWNTPAWTMCTEAFFYLIFPAVIVWKRPKHWRGLLGLLAVLWV
                     AGMFLPALYTILHPDGPHPGRYTDGFWIRALKFSPPPHVPAFLFGIVLADVDALIARE
                     ARIRLWLGALGVAGLYIILYYGDHMPYAMMHDGLLMPLFALAILGLAGRNLIAHFFGF
                     APFVAVGQASYCLYILHFNLWDIIHRSHVLESTRLAAFDPWISYFLLIAAALLTMKYI
                     ERPAQFWIRRRIPE"
     gene            complement(9065..>9562)
                     /locus_tag="VFA02_14005"
     CDS             complement(9065..>9562)
                     /locus_tag="VFA02_14005"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HZU11014.1"
                     /translation="DHMGMLATVINSLAIQDALEKKGVHARVMSAIEMPQVCEPYIRR
                     RAIRHLEKGRVVIFAAGTGNPYFSTDTAASLRAMEIKADVLLKATKVEGIYDADPVIK
                     TDAVKFEQITYMEILRLGLKVMDTTAVSLCKDNNLPMIIFNLNQPGNIRRVICGEKVG
                     SLVTA"
BASE COUNT         1998 a         2760 c         2660 g         2144 t
ORIGIN      
        1 gtatcggccc agcttccgta atcatccaga tcgtcgtatc caaccacgcc ccggcctaca
       61 tactgcacag agcgcgcatt cagaatatgg tggtcgcggt caatactcca gcggtcaaac
      121 gcatcgagac cgggcatctg ctgcgacaca agctggatgg gattggtgcc cgtgagttgc
      181 accgactggc cagcgccgac gtactgcgaa actcccgggc ctgtgacctc taattgaccg
      241 ctgttgacag tgactatagt tccgccatcg cctgtatatg aatccacgcg gaaatctccg
      301 ggctgcgtga ccatcagtgt gccgttgggc gtgtccactt caacctgctc accgggattc
      361 agcgagaaag tatgcacgcg aagactgccc tgcgcaaggc ccatctgggt caactggtcg
      421 gccaggttcg tcattgtcag gtctgtggtc tgccagacgc gcgccacaag attgcccatc
      481 tgaatctcgg cgcgtgaatt ctggtcggag taaatgcggt ctccagtaga aatgggatag
      541 ttgttcgcag cctgcgtcca ttgctgctcg tcagaaggct gaatggaaac attcccctgg
      601 accgcatgaa ggcgcgcgac gcggaccgag gggtctcccg agtcggcgtg aagaagcgct
      661 gtgctgaaca caacagcaaa agccgcaaag agaatgcgtt tggccaggta cgtcatcttt
      721 ccctcctggt atggtccggc ccaatctgtt ttaacacgta agttaccggg atgatgcggg
      781 cgacaaaaag aggcttcgca cggcgggaag cctcttgaaa attgctccgt atggatggct
      841 gccatctgcc gacgcaatct ttgcctccgt ttgcggtccc tccctaaatg gaccttcgga
      901 attgcgtctg gactactaat agaagcaaat ccagcagccg tcctatatgt tgtatccgct
      961 atgcagatat gtcaacaaaa atctgcacaa aaaaatgcgg cggaacatta gacctctccg
     1021 cggcgtatgt ttttgttaga aagaaggtct tatgaagacg ttgtttcttt ctgctttggc
     1081 agcttcactg ctggctgttg ccggatgccg catccaggtg aacaaagcaa aaaatgggga
     1141 agacaagacc gtaaagattg acacgcccat gggcggactt cacgtccgca caaacgaaat
     1201 gagcgccgcc gatgtgggtt tgcctgttta tccaggcgcg caagtggtca cggacagtaa
     1261 agaaaaagat gacggcaaaa acaaatcagt ggacgtccac atgggtttcg ggaaatggca
     1321 gctccgcgtg aaggtcatca attatcagac ctccgactcg caggacaacg ttctcgcctt
     1381 ctaccgcaag gccctgggcc gctttggcga cgtgatcgag tgcaataaca atactccggt
     1441 cggcacaccg gccagaactc atgaaggact cacctgctca gagcatgcaa agaactccaa
     1501 cgtgcatgaa gctgacgacg gccggagtct gcgtgctgga tcgccacacc accagcacat
     1561 tgtgagcatt gacaagaaca gtacggccgg aaaaacacaa ttcgcgttaa ttgagctgga
     1621 gctgcccgaa ggcgctgaga tttctgatga cagccagtaa tttcttccgg cccggtggtc
     1681 tttcttaaat cgggtgtttg cgaattcctt cctcagtacg atagggtcga agtatggatc
     1741 cggtttgcca ccgctgcggc aactcgctca gttcgtctga aattttctgc ccgcattgcg
     1801 gagcgccgca gttgcgcttt gagccttctg atgaacagcc ggtccaacgc ccccagaccg
     1861 tcggcgcacc cttccgtatc atccagcgtg tggagtggcg tgcgaccgta caggcagcgc
     1921 tgatgctcgc cctgccagtg ggactgctca gctcactgtg ggacttcgga attttatgga
     1981 tgcttgccgg aggcctctgc accgtcgcac tttaccgcaa gcgaaccatg aacaccgtga
     2041 atgccggcat cggctggcgc atcggcatgt tgaccggact attgtcggca tactgcgcaa
     2101 gcctgataga tggcctcggg atggtgctcg aacgctatgg cctgcatcac ggcgcgcgca
     2161 ttgaccagcg cctggctgcc atgacacagc agatgaccga acagatggcc cgccagaacc
     2221 ccgacgccgc acagcagatt ccctggtttc tgcacttctg gctttcgccc gatggacacg
     2281 ccgcgatggt cctgctcatg tccagctttg cggccgtcat gatgatcgct ttttcagcca
     2341 ttggcggagt gctcggggcg cgcatattcg gcgcgcggca tcgcctgtcc acgaatttgt
     2401 gatgcaggaa tacaactgct ctacatacgg tgacaggatt gcgcatctct acgatgacct
     2461 gaggcaattc acgcctgaac agacacagac cgaagtggac gctctggccg ccctggcaaa
     2521 gggcgggcct gtgctggaac tgggcattgg gacaggacgg gttgcccttc cgctcaaaga
     2581 aaagggcctc gaagtccacg gcatagatgc ctccccgaaa atgattgaag agctgcgcag
     2641 gaagcccggt ggcagtcaaa ttcctgttgc catcggcgat ttcagcagcg tgcccgtcga
     2701 agggcgattc agcctcatct ttgtcacatt taatacgttc ttcgctctga ccacccagga
     2761 agcccaggtt cgatgctttc gcagtgttgc cgcgcatctc gcgcctgacg gcctgtttgt
     2821 aattgaagct tttgtgcccg atccttcacg ctttacccgc aaccaaaaca ttgtcgtgtc
     2881 aagagtggac ctggacgagg tacgcttcga tgtttcacaa catgatcctc tgcaccagcg
     2941 cgtagttgcg cagcacgtcg tgattacaca gggaaacacg caactctttc ccgtgcagat
     3001 acgatacgca tggcccagcg aactcgacct gatggcacaa cttgccggga tgaaactgaa
     3061 agatcgctgg agcacctggc agggggcacc atttacatcg ggcagtacag cgcatgtttc
     3121 tgtgtatgag ctgatgtaga gatcgcagag actgcacggt cacgccgcgt tcctgtacat
     3181 cctccacctg tcagctgcgc gccctgtacg cttctctctg taagctgtac cctgtacgca
     3241 acagatgaaa cgcttcctgc tctccattct cggactctgg ctcctgctct acggctcctt
     3301 tactcttgtc cgtccgccgc tgtttgatgg agtagatgca acacacgccg aaatcgcgcg
     3361 ggaaattctt gtcaccaatg actgggtcac attccacctg aacggagtgc ctttttttga
     3421 agcgccgccc ctgcttccct ggtgcatcgc tgtcagcttt aagatcttcg gattacatga
     3481 ctgggccgcg cggctgccgc ttgctttctc cgcgctggcg ttgtttctgg ttgtatttgc
     3541 gttgggccgc cgctgcttcg ggaacagcaa ggccggtttc tggtctgccg cttgcctgtt
     3601 gacggccagc ggcatcttcg gcttcatgca catgctgctt ccggaatccc tgttctgcct
     3661 ctggctgacg ctgagcatct attttttccg gaggtccctt gaatacgaac aggggcagcc
     3721 ttccatcacc tcggcctgcg ggtttgccat ctgctgcgcc ctcggcgtcc tcagcctggg
     3781 actcgtcggc atcattttcc ctgtcgccat cgttcttatc tatctcgggg ccacaaagga
     3841 cctgggccac atctttcgct ggcatccctg ggtgggactg cttgtcttcc tcgtcattgc
     3901 gctgccgtgg cacgttcggg ctgcgctcag aaaccctggc ttcctttggg catacttcgt
     3961 caacggccac tttctccgct atcttggccg tcacaccccg gccagtgaag atcccgtgcc
     4021 gctctggata ttctggcttc tgctgctgat atttatcgcg ccctggtgca tctttgccat
     4081 caaaatgctg gcgcgcctgc gctctgtccg gcagagcacg cttctgctcc cggccatctg
     4141 ggccggagtt gtcatgctct tttttacctt ctccttccgg caggaatact atcttctgcc
     4201 agctttgccg ccgctggcat tgctcgccgg cggatggctg gccgaagatg aagattctcc
     4261 ttcgcctgcg ggaagaatca ctgcctgggt cttattgatt gccggaacca ttgcggccat
     4321 cgcatcggcc atctttgccg tcacctttaa gcctttgccc atcgggacag acgtttcggc
     4381 ttttctgcag gaagtctcgc gcagccatcg cctcttcttc gggcatttct ttgacctcac
     4441 actgcgcgcg ctcggggcct tccgcgtgcc gctcaccatc acgaccgcag cgctgcttgt
     4501 aggcgtgacc gcgaatctct gcttccgtct cagaaagaga acaggccttg caaacgcgtt
     4561 cctgctgggg accgtcgttg cattcctcat cgcagcgcat ctggcgctga acacgctctc
     4621 tccggccatc tcttcgcaga ttctcgccaa cgccatcctg cctgagatgg attcagacga
     4681 tgtggtcatc gtcaacggcg cgtttgaaaa tgcgtcctcg ctcgtctttt atctgcgaag
     4741 gcaggcaaaa attctgaatg gacataaccg tcaaagccag atagaccaga cctccctcga
     4801 ccaactgtgg agcggccagg agcgtgtttt tctctggaca ccgaacgacc aggttccact
     4861 cctgccgcag ccgacgttcg taataggccg cagcggcgga aaagaaatcc tgagcaacca
     4921 gcccaactcc agaggggcgg agttttaagg caggtttctg tcccgtcctt tttcgcacat
     4981 aggcggcgcg aaaaaagcat ggtgcacccg gggattttcc ccacgcagac tcgcttgagt
     5041 gggccacccg ccacactggc atcagtctgc gcactcgaaa atcttctttg acagatatcg
     5101 ctccgctgaa tccggaacaa tcgtggcaat tcgttttcct tggccgagtc gtttggctac
     5161 gttcagagcg gcgtggacgt ttgccccggc acttgaccct gccagcacgc cttcttctgc
     5221 ggcgagccgt ctcaccatcg cgaatgcttc ggcatccatg acgcgtacaa tctcgtcggc
     5281 cacgctgcgg tcgaatgttt taggcacaaa ggaaacacca atgccttcca ctttgtgcgg
     5341 acctggctcg cccccctgaa gcactgaacc ttccgtctcg acggccacgg tcaggatgcg
     5401 cgcgtctttt tctttcagca gacgcgccac tccggagaag gtcccgcccg tgccgactcc
     5461 cgcaacgaag gcatctatgc ggccttccat ctgctcccaa agctcctggg ccgttgtttc
     5521 gtagtgaaat tgtggattgg cctgattatc aaactgcatg gcggggaaga tattcgggtc
     5581 tgttttggcc agcgcaagtg cttctcgaat ggccccactc atgccttcgg cttccggggt
     5641 gcgcacgacc ttcgcgccga atccgcgcat gagcatgcat ttttcttcgg cgtagccctc
     5701 cgggacgaag agcacaacct tatacccacg attgacgccg acgagcgcca gccctactcc
     5761 ggtatttcct gccgttgcct ctacgatggt tgagccgggc ttcagaatgc cgcgcgcctc
     5821 tgcatccaga atcatgccca gagcggcgcg gtctttcacg cttccccccg gattcaggaa
     5881 ctcgagcttg gcgtaaacat ctgccatttc aggagttacg atccggccaa ggcgcagcat
     5941 gggtgtttgt cccatgagtt cggtgacgtt ctctgcaatg cgcagggcgc gtacgggctg
     6001 aagggtggtg ggtgcgttca taacaaaact tctcagtgta gcagggaagc aggcagctgc
     6061 gttctgtccc gatcgggttt tacctcaaag aaaacaatgg tcatctgtaa acgtgtttat
     6121 tttctggcat atgactgcga atgtttatac actgaactgc aactaagcga ctttggagaa
     6181 tttaagggta tcgccgttaa agtccagaaa caggacactg ttccttctgg ccctgggggc
     6241 tgtggggtct gttgtcttcc tcacggcggc ggcgttcttt gtctatcaca ccacgaaaga
     6301 cctgctctcc gcgcgcgatt gggaagagca ttcccaggaa gttctgaata cgctccaact
     6361 gacctcgcag cgtctggatc gtctggatct gaccagcagg ctctattttg ctgaaaagaa
     6421 ccgtgatgac ttgggtgccg ctcagacggt ggccgtggtg ctggtcgcag gagtcagtca
     6481 tctgaccggc ctggtggggg acaatcctgc acaggaggcg ctggcccagg cgctgcagtc
     6541 ctgcacaagc gaactaaaac aaatcgcgga caagacttct ccggcagatg tcgccgggca
     6601 gagcattgtc ttcgggcaga aggtcctgga ttgcagagat tacgtcgtcc gaatgcaggc
     6661 cgatgaaaat atgctcctca ggcagcgcag ggacgagtcg cagcgagggg cctaccgcag
     6721 cctgagcgcg ggagctgttt tcctgcttgt ttcgctggtg accgttcttg tgctgtttgg
     6781 ccttctggtc cgggatgcgg cgcagcagta taaagcaaaa caggaagtct tcgatgcaaa
     6841 tgcgcggctg gcgtccaccg ttcaactcat ggagcagcgg gccgtggaag caggcctgct
     6901 tacggccacc caggaagagc tgcaactgtg caccaatgtg gcccaggcct accaggtcac
     6961 agtgcggcgg tgttcccagt tgctgccttc agcaagtatt gccttgctga tgatcaacaa
     7021 ctcgcgccag atgttggaac tggtcgcagc atcaggcggt ctcacccagg ttgtggaggg
     7081 tttttcgctg gatgcctgct gcggtctccg cggcggccag attcgctggc gcaaacgggg
     7141 gcagtcggag attgactgcg aacattttgt gggcgcgccg ccggaaaatt atctgtgtat
     7201 gccgctaacg gctcatgggg acacactcgg gatcttgtat gttgagtgct cacctgacga
     7261 agacatgcct cacattgaag cgaaaatgga ggcgttgaaa agcatcgtga aagtggcggc
     7321 ggtgttcttt ggcagcctga atcttcgcgc ccggcttgag aaccaatcca ttcgcgatgg
     7381 cctgaccaat ctcttcaatc gccacttcat ggagatatct ctcgaccgcg aaatacgccg
     7441 tgccgccaga agccgcaccg aactggccgt gctgatgatt gatgtggacc acttcaagca
     7501 ctttaatgac acatttggtc acgaggccgg cgattacgtc ctgcgcgaag tagcagaggt
     7561 attcaggaac actgtccggg ttgaagacat catctgtcgg tatggtggtg aggaatttgt
     7621 catcattttt cccgaaaccg gggccgaaag tgcaaagacg cgcgctgaag aaatccgcag
     7681 gcagatccag cggcttcaat tgcgttttcg tggagaaaat ctgcgcgaga ccacggtctc
     7741 catcggcatt gccgtctatc cggaagccgg aaactccctg gtggagctgc tgcgcaatgc
     7801 ggaccgtgcg ctctatgccg ctaagaatcg cggccgcaac tgtgttgtga cttcgttgac
     7861 tgaggtgacc gcgtaggctt ctactccgga atcctgcggc ggatccagaa ctgtgcaggg
     7921 cgttcaatgt acttcatcgt aagcaaggcc gcggcaatca acagaaaata gctgatccag
     7981 gggtcgaacg ctgccagccg tgtgctttcc agaacatgcg atcggtgaat aatgtcccac
     8041 agattgaagt gcagaatata gaggcaataa ctggcctgtc ctacggcgac aaacggggca
     8101 aacccaaaga aatgggcgat caggttgcgt ccggcaagac cgagaatcgc cagcgcaaac
     8161 agcggcatca gcaggccatc gtgcatcatg gcatagggca tgtggtcgcc gtaataaagg
     8221 atgatgtaaa gtccggcaac gcccaaagct ccaagccata aacgaatccg cgcctcacgc
     8281 gcaatcagag catccacatc cgccagcaca atgccaaaca ggaatgctgg cacatgcggc
     8341 ggaggagaga acttcagcgc acgtatccag aagccgtctg tgtaacgtcc agggtgcgga
     8401 ccatcgggat gaaggatggt gtatagcgct ggcagaaaca tccctgccac ccatagcacg
     8461 gcaagcagac cgagcagacc tcgccagtgc ttcggccttt tccagacaat gactgccgga
     8521 aagatgagat agaagaaggc ctccgtacac atcgtccagg ccggagtgtt ccagaatgta
     8581 gcgagcttcg gtatccatcc ctgcagaagc agtggggtga gtgtcgcgcc gaggaaaaat
     8641 tcgccccgtg tgcgcacgtg ccactcctca atcagcacgc caatcgaaac cagcagcgca
     8701 aacagataca ccggatacag acgcgaaagg cgcgccatcc agaagtgggt ggccttcagt
     8761 tggccatgct gtgcccgctc gctgtagttg taagccagga tgaacccgga catcagtaga
     8821 aagaaactga ccgatgtata gccgttgtcc acaataggcg cgaacggacc gaaccacttt
     8881 ggattcgaga aatggaagaa gactatattc agtgcagcaa aggcacgcag gccggagagc
     8941 gccggcagct tcggtttgct gatttttttt gaaactggct ttgctggttt cgaggacggt
     9001 gggggcgtgg cagcaggcgg atccagcacc gcagcccgcg tctccgcgat gtgcggttgt
     9061 gcgctcaggc agtcaccagt gagccgactt tctccccgca tatcacgcgg cggatgttcc
     9121 ccggctggtt gaggttgaag ataatcatgg gcaaattgtt gtctttgcac aggcttaccg
     9181 cggtggtgtc cattaccttt aaccccagcc gcagaatttc catgtacgta atctgctcaa
     9241 atttcactgc atcggttttg atgaccgggt cggcatcata gatgccctcg accttggtcg
     9301 ctttcagtaa gacgtcggcc ttgatctcca tggcgcgcaa ggaggccgcc gtatctgtgg
     9361 aaaagtatgg atttcccgtt ccagcggcaa aaatcaccac gcggcccttt tccaggtgcc
     9421 gaatggctcg gcgccggatg tacggctcgc acacttgcgg catctcaatg gcggacatca
     9481 cgcgcgcatg gacacccttc ttttcgagtg cgtcctgaat ggccagcgaa ttaatgacgg
     9541 tcgccagcat tcccatgtgg tc
//
LOCUS       DASRBV010000384         4738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_440857, whole genome shotgun sequence.
ACCESSION   DASRBV010000384 DASRBV010000000
VERSION     DASRBV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440857"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..764
                     /gene="phoU"
                     /locus_tag="VFA02_14010"
     CDS             93..764
                     /gene="phoU"
                     /locus_tag="VFA02_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZU11015.1"
                     /translation="MPRIHFQQQLAELKDKILAMAALSQQALEASVDAYLQRDPGLCQ
                     FVRDNEKAINAAQRELDEMAYDLLAKEQPMAIDLRFILAVIKINGDLERIGDQAMSIV
                     LRSAEMIEGDPVDLPLDIDGMRIHASRMIRTALQSLLDADAQVAESVLAMDDEVDRMN
                     RIAHQDLTKFIQQHPDLSQQALQAIIISRNLERIADHATNIAMDVIFWVRGADIRHQL
                     SAAVD"
     gene            complement(966..2678)
                     /locus_tag="VFA02_14015"
     CDS             complement(966..2678)
                     /locus_tag="VFA02_14015"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZU11016.1"
                     /translation="MAKVIRTKLSGFNLLNNPRLNKGTAFTERERDIFSLHGLLPPHI
                     GTLEDQIQRRKQVLDGLATNFEKYSYMRDLQDTNETLFYALLSRHIEQMLPIVYTPTV
                     GEGCQRFSEIWRKPRGLFLSYPNRHRIEQILSHPRYDCIRCIVVSDGERILGLGDQGA
                     GGMGIPIGKMALYTALGGIHPEHCLPVLLDVGTDNEERLNSPIYVGWRHKRVRGEEYD
                     GFVDAFVSAVKKRWPHILLQWEDFAGANASRLLTRYRDQLCTFNDDIQGTAAVATATL
                     LSAVNVTGIPLKDQKIIVLGFGTAGIGIANLLLAEMQEEGLSKEEAWQRFYAIDRLGL
                     LLEGGKDIRPEQQPFVRRKEEIQRWKLSNLNEIGLTDVVRNSGATVLIGVSGQQGAFT
                     EEAIRAIAKNSERPILFPLSNPTSRSEATPQQLLEWTEGRAIIGTGSPFPPVQIRGKQ
                     INIDQTNNSYIFPGLALGLIASRAKRVSDGMIMAAAKALAGLSPARRDKRASLLPALA
                     ESRKVGRIVAEAVGRQALAEKLAGVADEAAFLHQLEENIWEPEYLPYEREAEPEQPRI
                     QGKL"
     gene            complement(2915..>4738)
                     /locus_tag="VFA02_14020"
     CDS             complement(2915..>4738)
                     /locus_tag="VFA02_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZU11017.1"
                     /translation="AETILSAFVQKHPDSIFVSSIPVLQANLFLQEGDPQAALRILDA
                     HKGEAIANHADYQLALAKAYQMAGRSSDAAQMFRRVFLGFPLSNEAGVAKTQLAATGA
                     TSSLTVEERSRHADALYKAGRYSDAADEYRALANDPSVMGGQKNSLLVAAAHCDFKLK
                     RLSKEDLDRIPDSNDETGARRLYLLIEYARDKDDGETQRNLVLQMEQRFPQSQWLAEA
                     LYSSGNMYLLRKDYPTAITYYGELARRFPRHPYAPSAHWRAAWLNYRIRQYSEAARLM
                     DEQIAVYHGGKEIPSALYWRGRIYQDQEHRPEMAVAYYRTVARVYRHYYYAAIAQQRL
                     QELGSVKPATLAFLDSMQPETIPTLSDDIPDDDPHVVRAHLLANAGLNEYIAPEIQAA
                     DGSDEWGALAEAKIYASYGETWRAMRAMKRAIPFYTSAPVDALPMEYWRILFPQAYWS
                     TIKAESEKNRLDPYMVASLIRQESEFNPNAISRANAYGLMQLLPSVGRSMAKEEGIRH
                     FSTNDLLDPVTNIRLGTRYLRQTLDKFNNQPEYTFAAYNAGDNRVTDWQSIGSYTGID
                     EFVESIPFTETREYVQAILRNEEIYRELDHAQTAQAKSAPK"
BASE COUNT          990 a         1334 c         1338 g         1076 t
ORIGIN      
        1 gctgttccgg aatgggagca agtttggttc ccgtgagcaa cttttctcag cattgttaca
       61 tatctattgc atactgaaag ttggaggagt ccttgccccg catccacttt cagcaacagc
      121 tggccgaact gaaggacaaa attctggcga tggctgcgct ttcgcagcag gcgcttgagg
      181 cgtcggtaga cgcttatctc caacgcgacc caggactgtg ccagtttgtg cgtgacaacg
      241 aaaaggccat taatgcggca caacgggagc ttgatgagat ggcctatgat ctgctcgcca
      301 aagagcagcc catggccatt gacctgcgct ttattctcgc cgtcatcaaa atcaacggcg
      361 acctggagcg gattggcgac caggccatga gcattgtcct ccgttccgcc gagatgattg
      421 aaggcgatcc ggtagacctg ccgctggaca ttgatggaat gcgcatccat gcttcgcgca
      481 tgatccgcac ggcattgcag tcgctgctgg atgccgatgc gcaggtggcc gaatccgtcc
      541 tggccatgga tgatgaggtg gaccgcatga accgcatcgc ccaccaggac ctgacaaagt
      601 tcattcagca gcatccggat ctaagccagc aggcgctgca ggccatcatc atctcgcgca
      661 acctcgaacg aatcgccgac catgccacca atatcgccat ggatgtcatc ttctgggtgc
      721 gcggagccga catcagacac cagttgagcg cagccgtgga ttagttagag cgcttctcgc
      781 agcaggtatc gtccgtcgcc aggtttcagg aaaagaaggt catgattgag gcagggcggt
      841 acttccacct cataataaaa taaaggctta tagatagaga gtccgtcatt ctgacgactg
      901 cagggagtaa gaatctcagc aatgtgaggg ttttgccaat acaactaaga tttccgcgcc
      961 cgcaatcaaa gctttccctg gatccgcggc tgctctggtt ccgcttcccg ttcgtaggga
     1021 agatactctg gctcccaaat attttcttca agctgatgta ggaatgcggc ttcgtccgca
     1081 acgcctgcca gcttttctgc cagtgcctgc ctgcctacgg cctctgccac aatgcggccc
     1141 accttgcgcg actctgcgag tgctggcagc agacttgctc ttttatctct gcgtgcagga
     1201 gacaagccgg ccagcgcctt tgccgcggcc ataatcattc catctgacac acgctttgcg
     1261 cgcgaggcaa tgagcccaag cgccagtccg ggaaagatgt aggagttgtt ggtctggtca
     1321 atgttgatct gcttgccgcg tatctggact gggggaaatg ggcttcctgt tccgatgatc
     1381 gcgcggccct ctgtccactc cagcaactgc tgcggagtgg cttcgctgcg cgaagtgggg
     1441 ttcgataggg ggaaaagaat ggggcgttca gagttcttcg caatcgcccg gatggcctct
     1501 tccgtaaacg cgccctgctg cccggaaacc ccgatcagaa cggttgcccc gctgttgcgg
     1561 acaacgtctg tgagaccaat ttcgttcagg ttcgacagct tccagcgctg gatttcttct
     1621 ttcctgcgca caaatggctg ctgctcgggg cgaatgtcct tgccgccttc caggagcagg
     1681 cccaagcgat caatggcata gaaccgttgc caggcttctt ctttgctcag gccctcctcc
     1741 tgcatctctg ccagcagcag gttcgcaatt ccgatgcccg cggttccgaa tcccagcacg
     1801 atgatcttct ggtctttcag tggaatcccg gtgacattga ccgccgaaag cagggtcgcg
     1861 gtggcgaccg ccgctgtgcc ctgaatgtca tcattaaagg tgcagagctg gtcgcgatag
     1921 cgggtgagca aacgcgatgc gttggctccg gcaaagtcct cccactgaag aagaatgtgc
     1981 ggccagcgct ttttgactgc cgagacaaag gcatccacaa aaccgtcgta ttcctctccg
     2041 cgtacgcgct tgtgccgcca gcccacgtaa atcgggctgt tcaaccgctc ttcgttgtcc
     2101 gttccaacgt cgagcagaac aggcaggcaa tgctcgggat ggatgccgcc cagcgcagta
     2161 taaagagcca tcttgccgat ggggatgccc atgcctcccg cgccctggtc gcccagaccg
     2221 agaatgcgct cgccatcgct cacaacgatg caccgtatgc agtcataacg cggatgcgag
     2281 agtatctgct cgatgcggtg cctgttcgga tagctcagaa aaagaccgcg cggtttgcgc
     2341 cagatctcgc taaagcgctg gcagccttcg cccacagtcg gcgtgtagac aatcggcagc
     2401 atctgttcga tatggcgcga caacagggca tagaagagtg tctcattcgt gtcctgcagg
     2461 tcgcgcatgt agctgtactt ctcgaaattc gtggccagac catccaatac ctgcttgcgg
     2521 cgctgtatct ggtcttcgag cgtgccgata tgaggcggca gaagcccgtg gaggctgaag
     2581 atgtcgcgtt cgcgctcagt gaaagcagta cctttgttca gacgtggatt gttcagcagg
     2641 ttgaagccgg agagtttggt gcgaatcact ttggccatga gaaatacagc actaaggcat
     2701 tttcacagat tctgtagact ccatttgacc cgtgcagtgt cgccgactcc tgttggtcgc
     2761 ggcgactgta gtttcgaggc agggttcaga ctatctgtga aaatgctata ccccataagc
     2821 ataactgttg gttccatcag gaaaaattac aggtctgtct actgcgcgcc tgaccgcact
     2881 ttgcccaagc tgaaaaggca aatttatttt ctctttattt tggcgcgctt ttcgcctgtg
     2941 cggtctgtgc gtggtccagt tcacggtaaa tttcctcatt gcgaagaatg gcctgcacat
     3001 actcgcgcgt ttccgtgaac gggatcgatt cgacaaactc atctattccg gtatagctgc
     3061 cgatggattg ccaatcggtc acacgattgt ctccggcgtt gtaggcagca aaggtatatt
     3121 cgggttgatt gttgaatttg tccagcgtct gccgcagata gcgcgtaccc aggcggatat
     3181 tggtgaccgg atcaagcaaa tcattcgtcg aaaagtgccg gatgccttct tcctttgcca
     3241 tgctgcgtcc aactgaggga agaagctgca tcaatccata ggcattagcg cgggaaatcg
     3301 cattggggtt gaactccgat tcctgccgga tgagcgaagc gaccatatac ggatcaagcc
     3361 tgttcttttc cgactccgcc ttgatggtgg accaatacgc ctgcgggaag aggatgcgcc
     3421 agtactccat cggcagagcg tctaccggtg cggaggtata aaacggaatg gcgcgcttca
     3481 tggcacgcat ggcccgccag gtttctccat aagaagcgta aattttcgcc tccgccagag
     3541 caccccattc gtcgctgcca tcggctgcct gtatctccgg cgcgatatat tcgttcaacc
     3601 cggcattggc cagcaggtgc gcacgaacaa catgaggatc atcgtccgga atgtcgtccg
     3661 agagcgtggg aatcgtttcc ggctgcatgg aatcgaggaa agccaacgtt gccggcttta
     3721 cgcttcccag ttcctgaagc cgctgctgtg ctatcgccgc atagtaatag tggcgataca
     3781 cccgggccac ggtgcggtag tacgcgaccg ccatctccgg gcggtgctcc tggtcctggt
     3841 aaatacgccc gcgccagtac agcgccgagg gaatttcctt gcctccgtga taaacagcaa
     3901 tctgttcatc catcaggcgc gcggcctcgg aatattgccg aatgcggtaa ttaagccagg
     3961 cagcacgcca gtgcgcgctg ggagcatacg gatgacgggg aaagcggcgc gccagttcac
     4021 catagtatgt gatggccgtc gggtagtcct tgcgtagtag atacatattg ccgctcgaat
     4081 acagggcctc ggccaaccac tggctttgcg gaaagcgttg ctccatctga aggacgagat
     4141 tgcgttgcgt ctcgccatca tctttgtcgc gcgcgtactc tatcagcaaa tacaggcggc
     4201 gcgctccggt ttcgtcattg ctgtccggga tgcggtcgag atcctctttg ctgaggcgct
     4261 ttaacttgaa gtcacaatgc gcggctgcga caagcagcga atttttctga ccgcccataa
     4321 ccgatggatc gtttgccagc gcccggtatt catctgccgc atcgctgtag cgtcctgcct
     4381 tgtaaagagc atcggcgtgg cggctgcgct cttctacggt aagcgacgat gtagcccccg
     4441 tcgccgcaag ctgtgtcttg gcgaccccag cctcgttgct gagagggaat ccgagaaaga
     4501 cacggcgaaa catctgggcg gcatcactgg agcggccggc catctgatac gccttcgcga
     4561 gcgcaagctg gtaatcggcg tggttggcaa tagcctcgcc tttatgtgca tcgagaattc
     4621 gcaatgcggc ctgcgggtcg ccttcctgca gaaagaggtt tgcctgaagc accggaatac
     4681 tggaaacgaa aatgctgtcc gggtgcttct gcacaaaggc gctcaggatg gtctcggc
//
LOCUS       DASRBV010000385        10429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_550943, whole genome shotgun sequence.
ACCESSION   DASRBV010000385 DASRBV010000000
VERSION     DASRBV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550943"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="VFA02_14025"
     CDS             complement(<1..2285)
                     /locus_tag="VFA02_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU11018.1"
                     /translation="MLRTVLLSALLACPLCYASVTGPASAGASAAVATVTGTVTDTTG
                     AIIPGAQVQLMDATGALVTSATTDSTGHFRIQPPRTGNYTLTISLEGFQTATQMVHVG
                     ATAGPPMSVSLAVAAAVTQVEVNGNNNVDLTASEENADTAVMTADDLKQMPIFDNDYV
                     SAMSNFLDSGEGSTAGTGLMVDGVEANRAMVSPSAVEEVHINEDPYSARYYRPGRGQI
                     EIITKQAADAYHGQLNFFFRDASMNAQNAFAPNKPPEQRRIYEGSVTGPLWHAKNWAF
                     LGSFNRAEEDLNAIVNANVAPTPENPTGKYNVTVPAPTRDTEFSMRVGHQLTDKHSAS
                     LMYAFQDATNRNQGVGNQTLPEAGYNTETREDDVIFHDDYIVSPSILNQASIVLERSY
                     DPVSNVTEQPRINVRGYFTGGSAQNDQLRTEYNLRMNEIVSWVKGRNNLKFGINLPHL
                     GRRVLEDRTNSAGSYTYGPTYAPDGTVAVTAIQNYVAGNPSGFSIQQGKTRFVYHQQE
                     VGAFLQDQIKILPFFSLTPGLRYDWQNFLGGDTNNFSPRLSFALVLDQKNEMVLRGGG
                     GIYYDRIGSGPLADMARYEHADLRLLQVSSNQQPLCNPIEDCADLSALPPSLVEVAPE
                     IKTPYQIDYGISLDRKVGEKGVISIGGRMNRGIDLFRSVDVNAPLPPYYNERPDPEIS
                     QLRQIQSAGTQNGSALDINYRGRFNKYFTGFAWYTWSHYGNNTSGIYWFPENQYAPDA
                     EWGPADWDQRHHFGFYGMFNP"
     gene            complement(2381..3064)
                     /locus_tag="VFA02_14030"
     CDS             complement(2381..3064)
                     /locus_tag="VFA02_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZU11019.1"
                     /translation="MVQPIPAEKIRLIVFDLDGTLIDSRRDLANSINAMLTEFHRDPL
                     PEEIIAEYIGDGAGMLVRRALGDPADEKLVEDALASFLNHYRKHKLDYTYVYDGVFEA
                     LEALKPGRTLAVLTNKPVRPAQEICDALGLGPYFFRIYGGNSFATKKPDPEGLNTLIR
                     EAEVSPSETVMVGDSDVDILTARRAKTWAIGCRYGLSSHTVESIPSDSLADSPKDWIS
                     ALSAAKIEK"
     gene            complement(3209..4627)
                     /locus_tag="VFA02_14035"
     CDS             complement(3209..4627)
                     /locus_tag="VFA02_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZU11020.1"
                     /translation="MSTTPQSTTAAQLSLWASVREALRGSHQDYTAGSLNRAILLLAI
                     PMVLEMVLESLFAVVDVFWVGRLGADAVATVGLTESLLSLIFAIGIGLALSTTAMVAR
                     RIGEKDRDGAAVASVQAIFLGLFISLAIGVPCYIFAPRLLALMGGSPAIIATGTGYAR
                     IALGGGGVILMLFLNNAIFRGAGDAAIAMRLLWVSNILNLILDPCLIFGLGPFPRLGV
                     TGAALATFSGRSIGVLYQFYRLLRGTERIHVLMRHVRLQLSVLWRLLRVSLTGILQFA
                     IANTSWIAMVKIISHYGADALAGYTVAIRILIFIILPSWGLSNAAATLVGQNLGAKRP
                     DRAEQAVWQTGFYNMIFLGLIGVFFVFFATPVVRLFVTDSGVVPIAASALRLLSYGNI
                     GYAFAMVMLQAFNGAGDTVTPTIVNFFGFWMFQIPLALWLAFHTKMQAQGVFLAIVLA
                     EAAIAAASMLIFRRGKWKEQKI"
     gene            complement(4747..5487)
                     /locus_tag="VFA02_14040"
     CDS             complement(4747..5487)
                     /locus_tag="VFA02_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU11021.1"
                     /translation="MATQKVAFITGGNRGIGLETARQLGKIAGIHVVIGSRDLAKGKA
                     AADSLRADGADVEAIRFDITKSADYKEAYDFFDKKFGKLDILINNAGIAKENFFGGNN
                     TSKVSPAILRETFDTNFFGTVELTQTLLPLIKKAPEGRIVNLSSILGSLTLHADPNSP
                     IYNGKVLAYDASKAALNVFTIHLAHELRNTKIKVNSAHPGWVKTDMGTEAAPMEIPEG
                     AKTSVALATLPADGPTGAYIHLGKPLPW"
     gene            5568..8135
                     /gene="priA"
                     /locus_tag="VFA02_14045"
     CDS             5568..8135
                     /gene="priA"
                     /locus_tag="VFA02_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZU11022.1"
                     /translation="MPAFCDVALPVPLDRTFTYAVNGEVPDVGCRVLVPFRNEKMSGV
                     VVRVHDEAPPVEAKPLMTVLDHEPVISSVLLELALWIAQYYIAPIGEVLRGMLPLMAE
                     VRHVTLFRITDIGRQVLHEGAEKGSSRRSKLPPEEQKVEYAVLNYLENGEAAWASRLR
                     TATGASVALLQGMVRKKWIVRETEADARDAQRVVRYAVMVGVPLQNAGPSTPSPRDAG
                     RNELEFTDQDGKLRGDPLEGKRLPKLNENQQVILAELAGAGGELPVAELRKLDVPQST
                     LTTLVKRGLVVIEERPAEFHLTRLGVKPPEYELNFQQKAAFDEMTAAVASGEFRPHLL
                     HGVTGSGKTAVYLAAMRYALDAGKAAILLVPEIGLTPAMAAQLHHAFGEQVALLHSAL
                     TPQERAEQWHRIRRGEARIVVGTRSAVFAPVKDLGLIVVDEEHDSSYKQEETPRYHAR
                     DVAVMRAKLSSATVVLGSATPSLESWHNAERGRYAKIEMRERVNQRPLPVVELVDMRR
                     EFQETGQEHLFSRALIEETKATLERGEQAIILLNRRGYSFVVMCRACGHKLQCENCAI
                     SLTYHKAIGEDARAHVGQRLECHYCGYKRTVPRVCPECESEHLYFLGAGSQQGEERLQ
                     EIFPGARIGRMDRDTVRGRHDLERLLVRLHSGEINLLVGTQMIAKGHDIHGVTLVGVV
                     GCDHALSLPDFRAAERVFQLMTQVSGRAGRGELPGKVIVQTYYPDHYAIECSMKHDFH
                     GFAAKEMKYRRWMHYPPFNALANVLIQSPHLEEAAGWAATLGRWVQQTQLTGVRALGP
                     ATAPISRIKRIYRFHLILKSEKRQFLAQALRAMLAHAESQGIPRKNLVVDVDAVSLM"
     gene            complement(8164..8433)
                     /locus_tag="VFA02_14050"
     CDS             complement(8164..8433)
                     /locus_tag="VFA02_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZU11023.1"
                     /translation="MIKQDIVHHVIERTGLPRNKAEAAVDKVFDSLKKALTAGDRIEL
                     RGFGVFTVRPRKTGIGRNPRTGAEVTITPGKAVRFKPGKELHLTD"
     gene            8597..9151
                     /gene="dcd"
                     /locus_tag="VFA02_14055"
     CDS             8597..9151
                     /gene="dcd"
                     /locus_tag="VFA02_14055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZU11024.1"
                     /translation="MSIKSDRWIREMATKHGMIHPFSEKQVKEGVVSYGLSSYGYDLR
                     VSDEFKIFTNVNSAIIDPKQFDERSFVTVQAESVIIPPNSFALARSVEYFKIPRDVLT
                     ICVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPARVYANEGLCQILFFQSDE
                     VCEISYADRRGKYQKQQGIVLPKL"
     gene            9162..9824
                     /locus_tag="VFA02_14060"
     CDS             9162..9824
                     /locus_tag="VFA02_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU11025.1"
                     /translation="MDDQRQYGNGLRIGLFAVEPIRRAGLRSILEEMRSVELVETDAD
                     GLAQMDSLDVAVVSLTWPEEVLPIIRSLRSARPLMRIVVMTSECDDESMIAAISAGAK
                     GYLMDSATPEEVIKAIEIVASGSIWAPRHTLSMMVDRILAHGTLSAHSVPQFTQRELE
                     VLQLLVSARSNREIAQALGIEVRTVKSYIGRMMKKAGVGNRTALSVHAARNALVSPDR
                     SF"
     gene            10143..>10429
                     /locus_tag="VFA02_14065"
     CDS             10143..>10429
                     /locus_tag="VFA02_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZU11026.1"
                     /translation="MKRILTLLCIAGISLPVFAASDKAALDERLENSRTVIQEIMQTP
                     DKGIPDSIVKQATCIAVVPSLKKAAFIVGGQYGQGVVTCRTGRGWSGPVFIR"
BASE COUNT         2352 a         2613 c         3106 g         2358 t
ORIGIN      
        1 tccgggttaa acatgccata gaacccaaaa tgatggcgct ggtcccagtc ggcaggcccc
       61 cattccgcat caggagcata ttggttttcc ggaaaccagt aaatgccgct ggtgttgttc
      121 ccgtagtggc tccaggtata ccaggcaaag ccggtgaagt acttattgaa gcgtccacgg
      181 taattgatgt cgagtgcgct gccattctgg gtgccagcgg attgaatctg ccttagttgc
      241 gaaatctccg gatccggacg ctcgttatag taaggcggca acggagcatt tacgtctacc
      301 gaccggaaga ggtcaatacc acgattcatg cgtcctccaa tagaaatcac gcctttctcg
      361 ccaactttgc ggtcgagcga aattccgtaa tcaatctgat acggagtttt gatttcaggt
      421 gccacttcca ccagcgaagg cggaagcgca gaaagatcgg cgcagtcctc aataggattg
      481 cagaggggct gctggtttga agaaacctgt agcaggcgca ggtcagcgtg ttcgtatcgt
      541 gccatgtccg ccagcgggcc ggagccgatg cggtcgtagt agatgccgcc acctccgcgc
      601 agcaccattt cgttcttctg atccagaacc agcgcgaagg aaaggcgagg cgaaaagtta
      661 tttgtatctc cgcccaggaa gttctgccag tcatagcgaa ggccgggggt aagagaaaag
      721 aagggcagta ttttgatctg gtcctgaaga aacgcgccca cctcctgctg gtgatagaca
      781 aagcgtgtct taccttgctg aatggagaat cctgaaggat ttcccgccac gtaattctgg
      841 atggccgtta ctgccactgt gccatccgga gcatacgtag gcccgtaagt atagcttcca
      901 gcggagttgg tgcggtcttc cagaacgcgg cggcctaggt gcggaagatt gattccaaac
      961 ttgaggttgt tccgtccttt tacccaggac acaatctcat tcatgcgtag gttatattct
     1021 gtacgcagct gatcgttctg ggcactcccg cctgtgaagt agcctctcac attgatgcgc
     1081 ggctgctccg tcacattgga aacaggatcg tagcttcgct ccagcacaat tgaagcctgg
     1141 ttcagaatcg aaggcgagac gatgtaatca tcatggaaga tgacatcatc ttcgcgggtc
     1201 tccgtgttgt atccggcttc aggaagagtc tggttgccca caccctggtt gcggtttgtg
     1261 gcgtcctgaa acgcatacat cagcgaagca gaatgcttgt cagtcaattg atgaccgacg
     1321 cgcatggaaa attccgtatc gcgcgtagga gcaggtaccg tcacattgta tttgccggtt
     1381 ggattttccg gagtgggcgc tacattcgca ttcacaattg cgttcaggtc ttcttctgcg
     1441 cggttaaacg agccaagaaa cgcccagttc tttgcatgcc ataacggtcc cgttacgctg
     1501 ccttcataaa tgcggcgttg ttccggcggc ttattaggcg caaaggcgtt ctgagcgttc
     1561 atggatgcgt cccggaaaaa gaaattcagt tgtccatgat acgcatctgc ggcctgcttc
     1621 gtgatgatct caatctgccc gcgtccgggg cgatagtaac gcgccgaata agggtcttca
     1681 ttgatatgca cttcctccac ggcagaagga gaaaccatcg cccggttggc ttccacgcca
     1741 tctaccatca ggcctgtccc tgcagtggac ccctctccgg aatcaaggaa gttgctcatc
     1801 gccgaaacgt aatcattgtc gaagattggc atctgcttca ggtcatctgc ggtcatcacg
     1861 gccgtgtctg cattttcttc tgaagcggtc aggtcaacgt tgttgtttcc attgacttca
     1921 acttgcgtaa ctgccgctgc aaccgcaaga gagacactca tcggagggcc ggcagtcgcg
     1981 ccaacatgta ccatctgtgt tgccgtctgg aagccttcaa ggctgatggt cagcgtgtaa
     2041 ttgcctgtgc gtggaggctg aatgcgaaaa tgcccagtgc tgtctgtggt ggccgatgtt
     2101 acaagcgcgc ctgtcgcatc cattagttgg acttgagcgc cgggaatgat cgctccggtt
     2161 gtgtccgtaa ctgtgccggt aacggtggca acagcggcgc tggctcccgc agaagctggt
     2221 ccggtcacgg atgcatagca taatgggcag gcaagaaggg cagaaagcag aacagtgcgc
     2281 agcaatgcac ggtctccttt tcgaaaagtt tttaaaaatt tgcgatgttt ggccttaccg
     2341 gaaaaccagg aactccgaaa ttgttctttt tataaacgtt ttatttttcg attttcgcag
     2401 cacttaacgc agaaatccag tcttttggtg aatccgccaa agaatcagat ggtatggatt
     2461 ccactgtatg cgaagagagg ccataacggc aaccaatggc ccaggttttt gcgcggcgcg
     2521 ccgtcagaat gtccacatct gaatcgccga ccatgacggt ctcgctgggc gagacttcgg
     2581 cctcgcggat gagggtgttc agcccttccg ggtccggctt tttcgttgca aagctgttgc
     2641 cgccataaat acggaaaaaa taaggaccca ggccgagagc atcgcagatt tcctgggccg
     2701 ggcgtactgg cttgttggtc agcacggcga gtgtgcgtcc aggcttcagc gcttcgagcg
     2761 cctcaaagac gccatcatac acgtaggtgt agtccagctt gtgcttgcgg tagtgattca
     2821 gaaaagaggc cagggcgtct tctaccagct tttcatccgc tggatcaccg agcgcgcgcc
     2881 gcacgagcat tccggcacca tcgccaatgt actcggcgat gatttcttcg ggcagcggat
     2941 cgcgatgaaa ctcagtcagc atggcattga tggagttggc gaggtctctg cgcgagtcaa
     3001 tcagtgtgcc gtcgagatca aaaacaataa ggcggatttt ttcagcgggg atgggctgca
     3061 ccatggttcc agtgtaacga gagagcatgc actgattcga tcgcttgccc ggtcctgttt
     3121 acagaccctg attccctgaa cttgaatgtg ccaaaaagga cgtccaaggt agaggaagct
     3181 tgctacatct gcagccaggt taaagttttc agatcttctg ttctttccac tttcctctgc
     3241 ggaagatcag catactggct gctgcaatgg ctgcctcagc caacacaatc gccagaaaaa
     3301 cgccttgtgc ctgcattttt gtatggaagg cgagccacaa cgccagcgga atctgaaaca
     3361 tccagaagcc gaagaagttc acaatcgttg gtgtgaccgt atcacctgcg ccgttaaatg
     3421 cctggagcat gaccatagca aatgcgtagc caatgtttcc atagctgagc aggcgcaagg
     3481 ctgacgcggc tatcggcaca acaccggaat ccgtaacgaa gagcctgacc actggcgtcg
     3541 caaagaagac aaagaacacg ccaatcaatc caagaaagat catgttgtaa aagcctgttt
     3601 gccagacggc ctgttccgcg cggtccggac gcttcgcgcc caggttctgt ccaaccagcg
     3661 tggcagcggc attactcagc ccccatgaag gcagaatgat aaagatgaga atgcgaatgg
     3721 cgacggtgta tcctgccaga gcatctgcgc cataatgcga aatgatcttg accatggcga
     3781 tccagctcgt gttcgcaatg gcgaactgca atatcccggt gagcgacacg cgcagcaaac
     3841 gccagaggac actcagttgc aggcgaacat gtcgcatcag aacatgaatg cgctccgttc
     3901 cgcgcagaag acggtaaaac tgatacagca cgccaatgct gcgtccgctg aaggttgcca
     3961 gtgccgctcc agtgacgcca agtctgggaa acggcccgag gccgaagatg aggcaggggt
     4021 cgagtatcag attaagaatg tttgaaaccc acagcagccg catcgcaata gctgcatcac
     4081 cagctccgcg aaagatggca ttgttcagaa agagcatcag aatcacgccg cctcctccaa
     4141 gagcaatccg cgcatagccg gttccggttg cgatgattgc aggcgagcca cccatcagcg
     4201 ccaggagtcg cggcgcgaag atataacagg gaactccgat agcaagcgag atgaacaggc
     4261 caagaaaaat ggcctgtaca gaagccaccg ccgcgccatc ccggtctttt tctccaatac
     4321 ggcgtgccac cattgccgtc gtggacaggg ccaggccaat gccgatggca aagatgagcg
     4381 acaggagaga ttcggtaagc ccaactgtgg ccacagcatc tgcgccaagg cgtcctaccc
     4441 agaaaacgtc caccacagca aagagcgatt cgagcaccat ctcaagcacc atgggaatgg
     4501 ccagcagcag aatcgcgcgg ttcaggcttc ctgctgtgta gtcctggtgt gagccgcgga
     4561 gtgcctcgcg cacagaggcc cacagagata gttgtgcagc agttgtagat tggggcgttg
     4621 tactcataaa gacaggtttc agaagtgtac agggaagggc gcagggtttg cgtgaagaag
     4681 aagtggaatt ccgggcgcca tcctggcgca ctctttgccg cgataggata aacgccggac
     4741 cttcatttac caggggagag gcttgcccag gtggatgtaa gcccctgtcg ggccatcggc
     4801 tggaagagtg gccagggcga cgctggtttt tgcaccttcg ggaatttcca tgggggcggc
     4861 ctctgtaccc atgtccgtct tgacccaacc tggatgcgcc gagttgactt tgatcttcgt
     4921 attgcgcagt tcgtgtgcca ggtggatggt gaaaacattc agcgcggctt tcgacgcgtc
     4981 atacgccaat actttgccat tgtaaatggg agaatttgga tcagcgtgca acgtgagtga
     5041 tccaagaatg ctggaaaggt ttacgatgcg gccttccggg gcctttttaa taagcggcag
     5101 aagagtctga gttaattcca ccgtaccaaa gaagtttgta tcaaatgtct cgcgaaggat
     5161 ggcaggggaa actttgctgg tgttgttgcc gccaaagaag ttttctttcg caatgcctgc
     5221 gttgttgatg aggatgtcga gttttccaaa ttttttgtcg aagaaatcgt aggcttcctt
     5281 ataatcagcg gatttggtga tgtcgaagcg gatggcttcg acgtcggcgc catctgcgcg
     5341 cagcgaatct gcggcggctt tgcctttggc caggtcgcgt gagccgatca cgacgtgaat
     5401 gcctgcgatt ttgccaagct gccgggcggt ttcgaggcca atgccacggt ttccgccggt
     5461 gatgaatgca actttttgtg tagccacggg tcttcctgga ttcgttgaat tcgatgttgt
     5521 gatggcagag gaggattcat ggtccggatt tttgaaagaa tagtgccatg ccggcattct
     5581 gcgacgtagc cctgcctgtc ccgctggacc gcacctttac ttatgcggtg aatggcgaag
     5641 tcccggatgt gggctgccgg gtacttgttc ctttccgcaa tgaaaagatg agtggagtgg
     5701 tagtgcgggt gcatgatgag gcgccgcccg tagaagcaaa gccgttgatg actgtgctcg
     5761 accatgagcc ggtgatatcc agtgttctgc tggaactagc gctgtggatt gcgcagtact
     5821 atatcgctcc aatcggtgag gtgctgcgtg gaatgctgcc gctgatggct gaggtacgcc
     5881 atgtaacgct gttccgcatc acggacattg gacgacaggt gctccatgaa ggtgcggaga
     5941 agggatcatc aagacgctcg aagttgcctc cggaagagca gaaggtcgag tatgctgttc
     6001 tgaattatct ggaaaacgga gaagcggcgt gggcctcgcg cttgcgaacg gctacgggag
     6061 caagcgttgc gttgcttcag ggaatggtgc gcaagaagtg gatcgtgcgt gagactgaag
     6121 ctgatgcgcg ggatgcgcaa agagtggtgc gctatgcagt gatggttggt gtgccactgc
     6181 aaaatgcagg tccttccact ccatcgccgc gcgatgccgg ccggaacgag ctggaattca
     6241 ctgatcagga tggcaaatta cgcggagatc cgctcgaagg gaagcggctg ccgaagctga
     6301 atgaaaatca gcaggtgatt ctggcggagc tggctggcgc cggcggcgaa cttccggttg
     6361 cggagctacg caaactggat gttccgcagt caacacttac gacgcttgtg aagcgtggtc
     6421 tggttgtgat cgaggagcgt ccagcagaat ttcatctgac gcggctgggt gtgaaaccgc
     6481 cggaatatga attgaatttc cagcagaaag cagcgttcga tgagatgacg gcagcggttg
     6541 cttcgggaga gtttcgtccg catttactgc atggcgtgac cggatccggg aagacggccg
     6601 tgtatctggc ggcgatgcgg tatgcgcttg atgcgggcaa ggctgcgatt ctgctggtgc
     6661 cggagattgg tctgactcca gcgatggcgg cgcaactgca ccatgctttt ggtgagcagg
     6721 tggcgctgct gcactcggcg ctgactccgc aggagcgggc cgagcagtgg caccgcattc
     6781 gccgaggcga ggcacggatt gtcgttggta cacgctctgc ggtttttgct ccggtgaagg
     6841 acctgggtct gattgtggtg gatgaggagc acgattccag ctacaagcag gaagaaacgc
     6901 cgcgctatca tgcgcgcgat gtggcggtga tgcgggcgaa attatcatct gcaacggtgg
     6961 tgctggggtc ggccacgcca tcgctggaat catggcacaa tgcggagcgt ggacgttacg
     7021 cgaagattga gatgcgtgag cgcgtgaacc agcgtccgct gcctgttgtt gaactggtgg
     7081 acatgcgtcg cgagttccag gagacagggc aggagcatct gttttcgcgc gcgctgatag
     7141 aagaaacgaa ggccactctg gagcgcggcg agcaggcaat cattttattg aaccggcgcg
     7201 gatactcgtt cgttgtgatg tgccgcgcgt gcgggcacaa gctccagtgc gagaactgcg
     7261 cgatttcgct gacctatcat aaagccattg gagaggacgc gcgggcgcat gtgggccaac
     7321 ggctggaatg tcactattgc ggctacaaaa gaacggtgcc gcgtgtttgt ccggagtgcg
     7381 agagcgaaca cctttacttc ctgggcgcag gctcgcagca gggcgaagag aggttgcagg
     7441 agatctttcc cggagcgcgt attgggcgta tggaccgcga cactgtacgg gggcggcatg
     7501 atctggagcg gctgctggtg cggctgcatt ctggggagat caatctgctg gtcggtacgc
     7561 agatgatcgc caaagggcac gatattcatg gggtcacgct ggtaggtgtg gtgggatgtg
     7621 accacgcgct gagtctgcca gattttcgcg cagccgagcg tgtctttcag ttgatgacgc
     7681 aagtctcggg ccgcgccgga cgcggagagc ttccgggcaa agtcattgtg cagacgtatt
     7741 acccggacca ttacgcgatt gagtgctcga tgaagcacga ctttcatggg tttgcagcaa
     7801 aagagatgaa gtatcgcagg tggatgcact atccgccctt caatgcgctg gcgaatgtgc
     7861 tgattcagag tccgcatctg gaggaggctg ccggatgggc cgccacactt ggccgatggg
     7921 tgcagcagac ccagttgacg ggcgtgcgag cgctgggtcc ggcaacggct ccgatatcaa
     7981 gaatcaagcg catttaccgg tttcatctga ttctgaaatc ggaaaagcgg cagttcctgg
     8041 cacaggcgtt gagggcgatg ctggcgcatg cggagagcca ggggattccg cgtaaaaacc
     8101 ttgtagtaga tgtggacgct gtaagcctga tgtgaacagg ccgcagcggc gccgcggtca
     8161 ggttcaatcc gtcagatgca gttccttgcc cggtttgaag cggacggcct tgcctggagt
     8221 aatggtcact tctgctccgg tgcgcgggtt gcgtccaatt ccggttttgc gagggcgaac
     8281 ggtgaaaact ccgaagccgc gcaattcaat gcggtcgccg gcagtcaggg cctttttgag
     8341 gctgtcaaag accttgtcaa cagccgcctc cgccttgttt cgtggcagcc cggtgcgttc
     8401 aatcacatga tggacaatat cctgctttat caaggcgttt ctcctttttc ttcaaggtcc
     8461 tggtgcaaaa tttccagact actgtggatg gtagaaaggt tctctggcat tgtcaacgcg
     8521 cctgttgtca cataagatgc acatttcagg caaattatct gtgatacaca taaaaagaag
     8581 gcaggcaggg agttcgatgt ccattaagag tgaccgctgg atccgggaaa tggccacgaa
     8641 acatgggatg atccatcctt tcagcgaaaa gcaggtgaag gaaggggttg tgtcctatgg
     8701 attgtcgtct tacgggtacg atttacgggt ttccgatgaa tttaagatat ttacgaacgt
     8761 aaatagcgct attattgacc ctaagcagtt cgacgagcgg tcttttgtga ccgtgcaggc
     8821 cgaatcggtg attattccgc cgaattcatt tgcattggcg cgctcggtcg agtattttaa
     8881 gattccccgt gatgtgttga cgatctgcgt agggaaatcc acctatgcgc gttgcggaat
     8941 catcgtaaat gtgactccct ttgagcccga atgggaaggg tatgtcacgc tggagatatc
     9001 aaacacgacg cctttaccgg cgcgtgtgta cgcaaatgag ggactttgcc agatcctctt
     9061 tttccagtcg gatgaagtct gtgaaatcag ttacgccgac cgcaggggaa aataccagaa
     9121 gcagcaggga attgtgctgc cgaagttata gcaagtagtg catggacgac cagcgccaat
     9181 acgggaacgg tttacgaatc gggctttttg ctgttgagcc aatacgccgc gccggactac
     9241 gaagcattct ggaagagatg cgcagcgttg agcttgtgga aacagatgcg gatggcctgg
     9301 cgcaaatgga ttcgctcgac gtggctgttg taagcctcac ctggccggaa gaggtgctgc
     9361 cgatcatccg tagcctgcgt tcggcgcggc ctttgatgcg tattgtggtc atgacctccg
     9421 aatgcgacga tgagagcatg attgccgcga tttcggccgg ggccaaaggg tatctgatgg
     9481 actcggcgac acccgaggaa gtcataaagg ccattgagat tgtggccagc ggctcgatct
     9541 gggccccaag gcatactctt tccatgatgg tggacaggat actggcgcat ggaacactga
     9601 gcgcgcattc ggttccacag tttacgcaga gagaactgga agtcctgcaa ttgctggtct
     9661 cggcgcgttc caatcgtgaa attgctcagg cgctcgggat tgaagtccgc accgtgaagt
     9721 cttacattgg ccgcatgatg aaaaaggccg gagtgggcaa ccgcactgct ctttccgtcc
     9781 acgcggcaag gaacgccctc gtatctccag accggagttt ctgaccccat aacgtttcag
     9841 tcagtggcag gtgacgaagg ttggcatacc ttcgttatct tgcgtagtaa tcagtattag
     9901 cttacaaacg taatcagcaa caacgcagat ctggggaacg gggctggcta gttcagcccc
     9961 cctttttttt gttcctgcgc cgaggtctcc cgcaagttac tacaaatcgt ctttccttgt
    10021 ctcaaccctc gcaacccggt tggcatccca tgtgtcagaa gttgaaacga cgggcggcgc
    10081 atctggaatg gcccgttata aaagaaaagg aattaaaaac agcatttgga gaaagaaaac
    10141 agatgaaaag aattctgact ttgctatgta tagcgggaat ttctcttccc gtgtttgccg
    10201 cgagtgataa agccgctctt gacgagcggt tggaaaattc ccggaccgtc attcaagaga
    10261 ttatgcagac cccggacaag ggtattccgg acagtatcgt gaaacaagcc acctgcatcg
    10321 ctgttgtgcc cagtctgaag aaggcggcct ttattgttgg cggacaatac gggcagggcg
    10381 tggttacctg ccgcacaggg cggggctgga gcggcccagt gtttatccg
//
LOCUS       DASRBV010000386         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_551170, whole genome shotgun sequence.
ACCESSION   DASRBV010000386 DASRBV010000000
VERSION     DASRBV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551170"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFA02_14070"
     CDS             complement(<1..894)
                     /locus_tag="VFA02_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU11027.1"
                     /translation="MATQTLAEPKLVAMERWRPRANPWAIAATVALAAFMEVLDTSIA
                     NVALPHIAGSLGASQDESTWVLTAYLVSNAVVLPMGGWAASIMGRKNFFMFCITVFTL
                     SSFLCGIAPTLGFLLIARVLQGFGGGGLQPMAQAIMADSFEPSKRGLAFALYGIVAIL
                     APSIGPTLGGWITDNYSWHWIFYINIPVGILALTLVQRMVDDPPWIKADRSRLRHADK
                     AGIALLILSMAALQITLDKGEEKDWFQSDFIRFFGITFAISFTALLVWEWFAKNPIME
                     LRLFKNRNFATCCFLMLFTGGL"
     gene            complement(1006..2016)
                     /locus_tag="VFA02_14075"
     CDS             complement(1006..2016)
                     /locus_tag="VFA02_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU11028.1"
                     /translation="MALVCVAQEPFPGGGSAQAPQQQPDQQPPPTIRVNTSVVLVPTL
                     VEKSSGEIVYGLTQKDFTLEDNGIKQNIHVDDDLDAQPVSLVVCIQRGRSAGLEFDKY
                     ARLGPLLDLFTAGGRGEAALVVFDSKPVYLEGFTRDTSTITDTLRQLRPGDGGAAILD
                     AVGYSVDLLEHQPPDHRRILLLISESRDHGSKRYSAQDLVERIGTSNTLVLGLTFSPT
                     KAGLMDWGKGNAQGGPSINVLAPLMMAVAAVKKNAARELAVMSGGEYAAFTKEKGFED
                     RIAELASHARNRYILSFRPSDLTPGMHRIEVKLNNDYNAHVIARASYWAVNDAAGGNS
                     MR"
     gene            2087..>3420
                     /locus_tag="VFA02_14080"
     CDS             2087..>3420
                     /locus_tag="VFA02_14080"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZU11029.1"
                     /translation="MRTALQSGNAAGTETELLVVFTTDVAAEKDAKPEIKLLTGEAAI
                     QQAASPFLSTGEFSSNSNEILLLHAPQGLKAKRLLLVGLGKAAKATVNHVRRAAGTAV
                     RFAKPRKLRDIAILAPADGLLTEATERAIAEGAILADFDPDTYRSDRKDQSVQSLTVI
                     IPAGANQKGAEEGLRAGTIIAESQNFTRSLVNEPGNRLTPTIFGQRAAEMAREFGLKC
                     EVYGAEKLHELKMGAFWSVAQGSDEPPALIVLTYDPEGAPDKPVLGLVGKGITFDTGG
                     ISIKPSDNMEKMKYDMAGGAAMIGAMRAIAQLKPRIKVIAVVCATENMPSGKAQKPGD
                     VQIAMSGKSIEIINTDAEGRLVLADGLAYAKQLGATHLIDAATLTGAVAVALGQINAG
                     AFSNDEDTWQEFSKAVAVAGERFWRLPLDEEYRDLIRSDIADIKNTGGRYGGA"
BASE COUNT          831 a         1023 c          897 g          669 t
ORIGIN      
        1 cagcccgcca gtaaacaaca tcagaaagca gcatgtagcg aagttcctgt tcttaaacag
       61 gcgcaactcc atgatggggt tctttgcaaa ccactcccac accagcaacg ccgtaaagga
      121 aatcgcaaag gtaataccaa agaaacgaat gaagtctgac tggaaccagt ctttctcttc
      181 gcctttgtcc agcgtaatct gcagcgccgc catcgagaga atcagcagcg caatgccggc
      241 cttatctgca tggcgcaggc gactgcggtc ggccttgatc cacggcggat catccaccat
      301 gcgttgcacc agagtcagcg ccagaatgcc aacgggaata ttgatgtaaa aaatccaatg
      361 ccagctatag ttgtccgtaa tccatccgcc cagcgttggg ccaatcgaag gagcaagaat
      421 ggcaacaatc ccatagagcg cgaaggccag gccacgcttc gacggttcaa acgagtcggc
      481 cataatggcc tgggccatcg gttgcaaacc gccgccgcca aacccttgaa gcacacgcgc
      541 aatcagcagg aaccccagcg ttggcgcaat gccacaaaga aacgagctta gcgtaaagac
      601 cgtaatgcag aacatgaaga agttcttgcg gcccataatg ctggctgccc atccgcccat
      661 gggcagaacg actgcattcg aaaccaggta agcagtcagt acccaggtgc tctcatcctg
      721 gcttgctccc aggctgcctg caatgtgcgg cagcgccaca ttcgcaatcg aggtgtccag
      781 cacctccatg aacgccgcaa gggccacagt ggcagcaatg gcccagggat tggcgcgcgg
      841 acgccatctc tccattgcga ctaatttggg ttctgcaagt gtttgcgtcg ccattctttc
      901 gcttttcgtt attgaatcag aacttcaata gatgcgaaat cctgctgcaa tgacgcaaag
      961 catggcctaa aaacagacgg atttgcaaat atttttcgag aaaattcagc gcatcgaatt
     1021 cccgcccgct gcatcattca ccgcccaata acttgcgcgt gcaatcacat gcgcgttgta
     1081 atcattgtta agcttcactt cgatgcgatg catccccgga gtaaggtctg acggacgaaa
     1141 actgagaata taacggttac gggcgtggct cgccagttct gcaatccggt cttcaaagcc
     1201 tttttcttta gtgaaagcgg cgtattctcc gccgctcatc acggccagct cccgcgctgc
     1261 attctttttc acggcggcaa ccgccatcat gagcggtgca agcacgttta tagacggtcc
     1321 gccctgggca tttccctttc cccagtccat aaggccggcc ttcgttggag aaaatgtgag
     1381 gcccaatacc agtgtattgc tcgttccgat gcgttccacc aggtcctgcg cgctgtaccg
     1441 tttgctgccg tggtcgcgcg attcgctgat gagcagcaga atgcgccgat ggtccggcgg
     1501 ctgatgctcc agcaggtcca ccgaatatcc caccgcatcc agaatggcgg cgcctccatc
     1561 tcccggacgt agctgtcgca atgtgtccgt aatcgtgctg gtgtcgcgcg tgaagccttc
     1621 cagatacaca ggttttgaat cgaaaacaac cagggcagct tcgccgcgcc ctcctgccgt
     1681 aaaaagatcg agcagcggtc caagcctcgc atatttatcg aactcaagcc ctgccgacct
     1741 cccgcgctgg atacaaacca ccagtgatac aggttgcgca tccagatcat cgtccacatg
     1801 gatgttctgc tttatgccgt tgtcttccag cgtgaagtct ttctgcgtaa gtccatagac
     1861 gatttcgcca gaggattttt caaccagcgt aggcacaagc acgacgctgg tattcacgcg
     1921 aatcgtggga ggcggctgct gatccggctg ctgttgtggt gcctgtgccg acccaccacc
     1981 cggaaatggc tcctgggcca cacaaaccaa tgccaccaca cagagacagc aacacagaca
     2041 tgcacggatg aggtaagaaa gcatctgctc cccttacact aaagctatga gaacagcact
     2101 gcaatccggc aatgccgccg gaacagaaac cgaactcctc gttgtcttta ccactgacgt
     2161 tgctgcggaa aaagatgcga agcccgaaat taagcttctt accggcgaag cagccatcca
     2221 gcaggcagcc tccccattcc tcagcaccgg agaattttcg agcaacagca atgagatcct
     2281 gctcctgcac gctccgcagg gactcaaggc gaagcgcctg cttctggttg gactgggcaa
     2341 ggccgcgaaa gcaacggtga accatgttcg cagggccgcc ggaaccgccg tccgctttgc
     2401 gaaaccgcgc aaactgcgag acatagccat tctcgccccc gcggatggcc tattgactga
     2461 agccaccgag cgcgccatag ccgaaggcgc gattcttgcc gacttcgacc cggacaccta
     2521 ccgcagcgac cgcaaagacc agagcgtgca atcgctcact gtcattattc ctgctggggc
     2581 caaccagaaa ggtgctgaag agggtctgcg cgcgggcacc atcattgccg aatcgcaaaa
     2641 cttcacgcgc tcacttgtca acgagccagg aaaccgcctg actcccacaa tttttggtca
     2701 aagagcagcg gaaatggcgc gcgaatttgg cctgaagtgc gaggtctacg gcgcggaaaa
     2761 actgcacgag ctgaagatgg gcgccttctg gagcgtcgcc caggggtctg atgagcctcc
     2821 cgcgctcatt gtgctgacct atgatcctga aggcgctccg gacaaacccg tattagggct
     2881 agtaggcaaa ggcatcacct tcgatactgg gggaatctcc atcaagcctt cagacaacat
     2941 ggagaagatg aagtacgaca tggctggagg agcagcgatg atcggcgcca tgcgtgccat
     3001 tgcgcaactg aagcccagga tcaaggtcat cgccgttgtc tgcgccacag agaacatgcc
     3061 ttccggcaaa gcgcagaaac ccggcgacgt acagatcgcc atgtcgggca aaagcatcga
     3121 aattatcaac acggacgccg aaggccgcct tgttctcgcc gacggcctcg catatgcaaa
     3181 acaacttggc gcaacacacc tgatagatgc agctacgctc acgggtgcgg tcgccgttgc
     3241 cctggggcag atcaatgccg gagcattttc aaatgatgaa gatacctggc aggagttcag
     3301 caaggctgtg gccgtcgcag gcgagcgctt ctggcgcctc ccgctcgatg aagagtaccg
     3361 cgacctgatc cgctccgata tcgctgacat caaaaacact ggcggacgct atggcggcgc
//
LOCUS       DASRBV010000387         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_441962, whole genome shotgun sequence.
ACCESSION   DASRBV010000387 DASRBV010000000
VERSION     DASRBV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441962"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1435)
                     /gene="xseA"
                     /locus_tag="VFA02_14085"
     CDS             complement(50..1435)
                     /gene="xseA"
                     /locus_tag="VFA02_14085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZU11030.1"
                     /translation="MSQAAQLGFTFEQSRHIWKVSELLGEVCAHLERQYADVWIEGEI
                     SNLRPASSGHIYFTLKDDEAQLGAVIFRRQASLLRFRPEDGLQVLVRGRVSVYEQRGQ
                     MQVIAEFMEPVGAGSLQIAFEQLKAKLQQEGLFDHERKKPLPAYPRCVGIVTSPTGAV
                     IQDFLNIVNRRHAALDVLLYPALVQGESAAQEIVSGIAYFNQARNVDVIVIARGGGSL
                     EDLAPFNSEPLARAIAASELPVVSAVGHETDFTIADFVADLRAPTPSAAAELITSALH
                     KVEEHVGLLALRLDRACRYRLLHARDLLARLNADAALARLREGFSRRQQHLDELRFRL
                     EGTVAGVLRRATVRLHEVSARLHRQDATHKLQFWRERVVALENRLAHALEARIRYGSA
                     TLDALTRHLHALSPLAVLQRGYALVYDENGTLVKQVARLSAGQQMTARLADGRVVGRV
                     TEIFPERTTTK"
     gene            complement(1447..1671)
                     /locus_tag="VFA02_14090"
     CDS             complement(1447..1671)
                     /locus_tag="VFA02_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HZU11031.1"
                     /translation="MSEMGRMFIGLGLMLLILGVVVLLLGKTGIPLGRLPGDISYRGK
                     NFSFYFPLGTCILISVVLSVLFYLFSRFRR"
     gene            complement(1686..2081)
                     /locus_tag="VFA02_14095"
     CDS             complement(1686..2081)
                     /locus_tag="VFA02_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU11032.1"
                     /translation="MEIKRAGSQASNKGPSEWFTGAVRIDPLFAAPAPARVQGASVTF
                     EPGARTAWHTHPLGQTLIVTAGCGWAQREGGPVEEIRPGDVVWFAPGEKHWHGATPTT
                     GMTHIAVQESLEGKVVEWLEHVSQEQYRK"
     gene            complement(2098..>2429)
                     /locus_tag="VFA02_14100"
     CDS             complement(2098..>2429)
                     /locus_tag="VFA02_14100"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HZU11033.1"
                     /translation="ISPFREDGVTYGTPTWIWSVVVDDALYARAYNGKKSRWYQAALQ
                     QKAGRITAAGMTKEVTFEPVDGPDSRQLQERIDDAYRKKYKGSPYLEPMIGVQARSAT
                     VRIVLRD"
BASE COUNT          529 a          805 c          614 g          481 t
ORIGIN      
        1 ggcgcttctc ctgctacgcc gcgaattcca tccgtaccaa atagtcttct catttcgtag
       61 ttgttctttc cggaaaaatt tctgtcacgc ggcccaccac acgcccatcg gccagacggg
      121 ccgtcatctg ctgtcctgca ctcaatcgtg caacctgctt caccagcgtt ccattttcgt
      181 cgtacacaag cgcatatccg cgctgcagga cggcgagcgg cgaaagcgca tgaaggtgcc
      241 gggtaagcgc atcgagtgtg gccgaaccat agcgtatccg ggcttccagc gcgtgtgcca
      301 gacggttctc gagcgcaacc actctctcgc gccagaattg cagcttgtgg gtcgcgtcct
      361 gccggtgcag ccgcgcagaa acttcatgca accgcaccgt ggcccggcgc agcaccccgg
      421 ccactgtccc ttccaggcgg aagcgcagct cgtccagatg ctgctggcgg cggctgaagc
      481 cctcccgcaa gcgggccagc gccgcatctg cattcaatcg tgccagcaga tcacgcgcat
      541 gaagcagccg gtagcggcag gcacggtcca ggcgcagtgc aagcaggccc acatgctctt
      601 ccaccttatg cagcgcagat gtaatcagct ccgccgcggc cgacggagta ggcgcgcgca
      661 ggtcggccac aaaatcggca atcgtaaagt ccgtctcgtg cccgacagcg gaaaccacag
      721 gaagctccga tgccgcaatc gcccgcgcca gtggctcaga attgaatggc gcaagatctt
      781 ccagcgaacc gccaccacgc gcaatcacaa ttacatctac attgcgggcc tggttgaaat
      841 atgcaatgcc agatacaatc tcctgcgcag cggactcgcc ctgcaccagt gccggataca
      901 acaaaacatc gagcgcagca tgcctgcggt tcacgatgtt cagaaagtcc tgaatgaccg
      961 ccccggtcgg cgaagtcaca atccccacgc agcgcggata cgcaggcagc ggttttttgc
     1021 gttcatggtc aaacagacct tcctgttgca attttgcttt cagttgctcg aaggcaattt
     1081 gcagcgaccc ggcgccaacc ggctccataa actccgcgat gacctgcatc tgcccgcgct
     1141 gctcgtaaac agaaacgcgc ccgcgcacca gcacctgtaa gccgtcttca ggccgaaatc
     1201 tcagcagcga tgcttgcctg cggaaaatca cggccccaag ctgcgcttcg tcatccttta
     1261 atgtgaaata aatgtgcccg gaagaagcgg gccgcaggtt ggatatttcg ccttcaatcc
     1321 agacgtcagc atactggcgt tcaaggtgcg cgcacacctc gcccagcagc tcagagacct
     1381 tccatatatg ccgcgactgt tcaaacgtaa agcccagttg tgccgcctga ctcacaattt
     1441 cttagtctac cgccggaaac gggagaacag atagaacaag acggaaagca caacgctgat
     1501 cagaatgcag gtgccaagcg gaaagtagaa ggagaaattc ttgccacggt aagagatgtc
     1561 ccccggcaga cggcccagcg gaatgcccgt cttgccaagc agaagaacaa cgacaccgag
     1621 aatgagcagc atcagcccca aaccaatgaa catgcggccc atctcactca tgaccacagt
     1681 ttaatttact tccggtattg ctcctggctg acgtgttcca accactccac caccttgccc
     1741 tctaaacttt cctggacggc aatgtgtgtc atgcccgtcg taggcgttgc tccgtgccaa
     1801 tgcttttccc ccggcgcgaa ccaaaccaca tcgccgggac ggatttcctc caccggacca
     1861 ccttctctct gtgcccatcc gcaaccagcc gtaacaatca gcgtctgccc cagcggatgc
     1921 gtatgccagg ctgtccgcgc tcccggctca aacgtcacac ttgctccctg tacccgcgcc
     1981 ggagcaggag cagcgaatag tggatcaatc cgcactgcgc cagtgaacca ctccgatggg
     2041 cctttgtttg atgcctgcga gccggcccgt ttgatttcca tctcttgttt ctccttatta
     2101 atcacgcagc acaatcctga ccgtcgcaga gcgcgcttgc acgccgatca tcggctccag
     2161 gtacggactg cctttgtatt tttttctgta agcatcatcg atgcgctctt gtaactgcct
     2221 gctgtccggc ccgtccaccg gctcaaacgt cacctctttc gtcatgccag ccgccgtaat
     2281 gcgtcccgcc ttctgctgca gggccgcctg ataccagcgt gacttcttcc cgttataagc
     2341 gcgggcataa agcgcatcat ccaccacaac ggaccatatc cacgtcggcg ttccgtaggt
     2401 cacgccgtcc tcccgaaaag gcgagatgt
//
LOCUS       DASRBV010000388         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_243871, whole genome shotgun sequence.
ACCESSION   DASRBV010000388 DASRBV010000000
VERSION     DASRBV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243871"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..453
                     /gene="groES"
                     /locus_tag="VFA02_14105"
     CDS             136..453
                     /gene="groES"
                     /locus_tag="VFA02_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZU11034.1"
                     /translation="MATATASVATTFTPLHDRILVRRVEESETVRGGIIIPDTAKEKP
                     QEGEVIAVGKGKQNEEGKVFPLDVKAGDRVLFGKYSGTEIKIDGEEFLIMREDEVLGI
                     LKK"
     gene            553..2217
                     /gene="groL"
                     /locus_tag="VFA02_14110"
     CDS             553..2217
                     /gene="groL"
                     /locus_tag="VFA02_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521902.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZU11035.1"
                     /translation="MAKQILHGEDSRQAILRGVNTLADAVKVTLGPKGRNVVIEKKFG
                     SPTITKDGVTVAKEIELKDALENMGAQMVREVASKTSDVAGDGTTTATVLAQAIYREG
                     VKTVAAGANPMALKRGIDKAVEAIVGKRDENGVVKGGALSKFSKPVSGDMIAQVGTIS
                     ANSDATIGNIIAEAMKKVGKDGVITVEESKTLETQLEVVEGMQFDRGYLSPYFVTDPD
                     RMEAVLEDPYILIYEKKISSMKDLLPLLEQIARNGKPLVIIAEDVEGEALATLVVNKL
                     RGTLNVAAVKAPGFGDRRKAMLGDIAILTGGKAITEDLGIKLENIKIEDLGRAKRVTI
                     DKDNTTIVEGRGDSKAIEGRVKEIRSQIEKTTSDYDREKLQERLAKLVGGVAVIKVGA
                     ATETEMKEKKARVEDAMHATRAAVEEGIVPGGGVALVRCAKDVDELIAKLEGDEKIGA
                     QIVRRAIEEPLRQIVGNAGEEGAVVVGKISENKDPNFGYNAGTGVYEDLVKAGVIDPT
                     KVTRTALQNAASIAGLMLTTEAMVSEIPEPKAAPAAPGHGGMGDMY"
     gene            2453..3838
                     /locus_tag="VFA02_14115"
     CDS             2453..3838
                     /locus_tag="VFA02_14115"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZU11036.1"
                     /translation="MIINLARRAHNHNWELDPITRSLLDTDFYKLLMLQFIWKHFPKT
                     KASFSFINRSAHVRLAETINMHELREQLEHTRRLRFHKSELIWLAGNTFYGQRSIFEP
                     AFLDWLEHGFRLSDYELSQKDGQIVLNFQGLWTETTMWEIYALTQVSELKTRASLKRL
                     SEFDLDILYARAKTRLWEKIARMRGVPCLSVSDFGTRRRHSFLWQEYVVKAMSDVLGD
                     SFKGTSNTYLAYKHDLEAIGTNAHELPMALAAMASSDEELKASQFRLLDLWQQTYQGE
                     LLVMLPDTFGTTQFLRNAPDWVADWTGQRADSKDPFIAGDEYIKWLEERGRDPRRKLF
                     IASDALDVDSILALHAYFSGSIQNGYAPSSFRSASDFLDQRKWKAERRIRFSAGWGTL
                     LTNDFRNCNPQGHDGFDPLSLVCKINDADGRPTVKLSDNYEKALGPAEEVERYRRVFG
                     TAGLTHLPLVV"
     gene            3970..5286
                     /gene="serS"
                     /locus_tag="VFA02_14120"
     CDS             3970..5286
                     /gene="serS"
                     /locus_tag="VFA02_14120"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZU11037.1"
                     /translation="MLDLAFVRANLPLVEEKLRARGQDPATLLGDFHVIDALRRERIT
                     EVEQLKSQRNKLSEEVGKLKKSGQDATSLMEQTRELKQKIDTLEASATQAEEQLRSIL
                     QRIPNLPHGSVPAGRSEHDNVEIKRWGKQPEFAFPPKPHWELGEALGILDLERAAKIS
                     GARFAVYWAEGARLERALINFMLDVHTREHGYTEVLPPFMVNSKSLFGTGQLPKFAED
                     LFRCGDEQGYKEAEYRDSDHWLIPTAEVPLTNLYRDEVLDEAKLTISLTAYTPCFRSE
                     AGSYGKDVRGIIRQHQFQKVELVKFAKPEESYNELEKLTANAEAILEKLELPYRRVLL
                     CTGDMGFGSAKTYDIEVWLPGQGLYREISSCSNTESFQARRANIRYRPQGKNKAEYVH
                     TLNGSGLAVGRTWLAILENYQQADGSVRVPDALVPYMGGQTVIAPR"
     gene            5429..5782
                     /locus_tag="VFA02_14125"
     CDS             5429..5782
                     /locus_tag="VFA02_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11038.1"
                     /translation="MGRILKSYFFWTYERGSFHYDVMVTLILAFIFVTPHLWNYGDHP
                     VPGNFAPTEVLVRADGRGGFTYVVPADQVQGGSLNTRLEAVIERISGPMVVDHYDTLR
                     DASGKVTAYKVWAHR"
     gene            5827..>6176
                     /locus_tag="VFA02_14130"
     CDS             5827..>6176
                     /locus_tag="VFA02_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HZU11039.1"
                     /translation="MRRFKTVFLSAAFAALIFAAASAHSQDDLSKVLSQMDAASQKFQ
                     SAQADFNWDQYTAVVQSHETQTGTIAFRRSGKNVEMVAHVKTDNGQPAPKDILYKNGQ
                     LTFYQPAIKQQTIFS"
BASE COUNT         1471 a         1832 c         1678 g         1195 t
ORIGIN      
        1 tctacattag cagtcggaga gtggaagtgc taaacctcta tcgagcacct cgtctgctct
       61 cgagaactta agtaattcct gaatttcagt actcaaaaac cgcgagcaaa tctcgcgcga
      121 aggagaagca ccgcaatggc aacagcaact gctagcgtag ccactacctt tactccgctg
      181 catgaccgca tcttggtccg gcgtgtggaa gagtccgaga ccgtacgggg cggcatcatc
      241 atcccggaca cagccaagga aaagccgcag gaaggcgaag tgatcgctgt gggcaaggga
      301 aaacagaacg aggaaggcaa ggtcttcccg cttgatgtga aggcgggcga ccgcgtcctg
      361 tttggcaaat actccggcac cgaaatcaag attgatggtg aggagttctt gatcatgcgt
      421 gaggacgaag tcctcggcat cctgaagaag taggactggc gtccaggcgg acgcggcttc
      481 gccgcaaaag cccggaaccg caatgtgagt tttaacggag catccagctc cgcctatgaa
      541 ggagaaacgg aaatggcaaa gcagattctg catggagaag attcacgtca ggccatcctg
      601 cgcggcgtga acactcttgc cgacgcagta aaggtgacgc tcggccccaa gggccgtaac
      661 gtcgtgattg agaagaaatt cggttcgccc accatcacta aagacggcgt gaccgttgcc
      721 aaggaaatcg aactgaagga cgcgctggaa aacatgggcg cacaaatggt ccgcgaggtc
      781 gcatccaaga cctccgatgt tgccggtgac ggcaccacca ccgcgaccgt tctggcccag
      841 gccatctatc gcgaaggcgt aaagacggtc gccgctggcg caaacccgat ggcgctgaag
      901 cgcggcattg acaaggccgt tgaggccatc gtcggcaagc gcgatgagaa cggcgtagtg
      961 aagggcgggg ccctctcgaa attctcaaag ccggtttccg gtgacatgat cgcccaggtc
     1021 ggcaccatct ccgccaacag cgacgcaacc attggcaaca tcattgccga ggccatgaag
     1081 aaggtcggca aggacggcgt cattaccgtc gaagagtcga agaccctcga aacccagctc
     1141 gaggtcgtgg aaggcatgca gtttgaccgc ggctatctga gcccgtattt cgtcactgat
     1201 ccggaccgca tggaagctgt cctcgaagat ccttacatcc tgatctatga gaagaagatt
     1261 agctcgatga aagatctgct tccgctgctg gagcagattg cccgtaatgg caagccactc
     1321 gtcatcattg cagaggacgt ggaaggcgaa gcgctcgcga cgctcgtcgt caataagctg
     1381 cgtggcaccc tgaacgttgc cgcggtgaag gcccctggct tcggcgaccg ccgcaaggcc
     1441 atgcttggcg acatcgccat cctcactggc ggcaaagcca tcaccgaaga cctgggcatc
     1501 aagcttgaga acatcaagat tgaggacctg ggccgtgcaa agcgcgtcac gattgacaag
     1561 gacaacacca cgatcgtcga aggccgtggc gattccaagg caatcgaagg ccgcgtgaag
     1621 gagatccgca gccagattga gaagacgacc tctgactatg accgcgagaa actccaggag
     1681 cgccttgcga agctggtcgg cggcgttgcc gtcatcaagg tcggtgcagc cactgagacc
     1741 gagatgaagg agaagaaggc ccgcgttgag gacgcaatgc acgcaacccg tgctgccgtg
     1801 gaagaaggca tcgttccggg tggcggtgtg gcgctggttc gctgcgcgaa agacgtggat
     1861 gagctgatcg cgaagctcga aggtgatgag aagatcggtg cccagatcgt ccgccgcgcc
     1921 attgaagagc cgctgcgcca gattgttggc aatgctggcg aagaaggcgc tgtggtcgta
     1981 ggcaagatca gcgaaaacaa ggacccgaac ttcggctaca acgccgggac tggtgtctat
     2041 gaagacctgg tgaaggcagg cgtcattgac ccgacaaagg tgacccgcac tgctctgcag
     2101 aatgcggcct ccattgccgg actgatgctc accaccgaag ccatggtttc ggagatcccc
     2161 gagccgaagg ctgctccggc agctcccgga cacggcggca tgggcgacat gtactaagga
     2221 accgccgcca ggcagacgcg gcatcgctgc aaaaaggccc tcgtctcgtg cgagggcctt
     2281 ttattgcaga aaattcctgg tttctaccct atcctccttc gtgcgctgtt tgcgtagtga
     2341 acacgaatct caacggggca atgtttcgtc cggttcgcac gcatttccat gcccaggact
     2401 gctttctctc tcaccctccc ctgccctgaa tttgaattaa agtaatttca tcgtgatcat
     2461 caatcttgcg cggcgcgccc ataaccataa ctgggaactc gaccctatca cccgctccct
     2521 gctcgacaca gatttttaca agctgctgat gctccagttc atctggaagc atttcccaaa
     2581 aacaaaggca tcgttctcct tcatcaaccg ttcggcgcat gtccgcctgg cagaaaccat
     2641 caacatgcac gaactgcgcg agcagttgga gcacacgcgc aggttgcgct ttcacaaatc
     2701 agaactcatc tggctcgccg gcaacacctt ctacggacag cgcagtatct ttgagccggc
     2761 gtttctcgac tggctggagc acggcttccg tctctccgat tacgagcttt cgcaaaaaga
     2821 cggacagatc gtcctcaact tccagggcct gtggaccgaa accacgatgt gggagatcta
     2881 cgctctcacc caggtaagtg aactgaaaac acgcgccagc cttaagcgcc tcagcgaatt
     2941 cgatctggac atcctctacg cccgtgccaa gacccgtctc tgggagaaaa ttgcgcgcat
     3001 gcgcggagtt ccctgcctga gtgtcagcga cttcggcacg cgccgccgtc acagctttct
     3061 ctggcaggag tacgtcgtta aggccatgag tgatgttctg ggcgacagct tcaaaggcac
     3121 gtcgaacaca tacctggcct acaagcacga cctcgaagcc attggcacaa acgcccatga
     3181 attgccgatg gctttggctg cgatggcctc ttctgatgaa gagctgaagg catcgcaatt
     3241 ccgcctcctt gatttgtggc aacagaccta tcagggcgag cttctcgtca tgctgccaga
     3301 caccttcggc acaacgcagt ttttgcgcaa tgctcccgat tgggtggccg actggaccgg
     3361 gcagcgcgcc gacagcaaag acccgttcat cgcaggcgat gaatacatca aatggctcga
     3421 agaacgcggt cgcgacccgc gcagaaagct cttcatcgca tctgatgcac tcgatgtaga
     3481 ctctatcctc gcgctgcacg cgtacttctc cggaagcatc cagaacggat acgccccttc
     3541 cagctttcgg agcgccagcg attttctcga tcaaagaaaa tggaaggccg agcgccgtat
     3601 ccgcttcagc gccggatggg gcactctgct caccaacgat ttccgcaatt gcaatcccca
     3661 gggacacgac ggcttcgacc cgctcagcct cgtttgcaag atcaacgacg ccgacggcag
     3721 gccaaccgtg aagctctcag acaactacga aaaagcactc ggccccgcag aagaagtcga
     3781 acgttatcgc cgtgtctttg gcacagcggg cctgacacac ctcccgctcg tggtctaagc
     3841 cgcatttcag aattttttgc caccctgagc gtagcgggaa gcgcgcagtc gaaggacctg
     3901 cttttctctg ttcctggaat accacatccc gaaaggatgg cgttcaccag atttagtaat
     3961 ctaaaagcta tgcttgatct tgcatttgtg agggccaacc tgccgttggt cgaagaaaaa
     4021 ctgcgtgcgc gcggacagga cccggccacg ctgctcggtg attttcacgt cattgacgca
     4081 ctgcgccgcg agcgcattac ggaagttgaa caattgaagt cgcagcgcaa caagctgagc
     4141 gaagaagtcg gaaagctgaa gaaatccggg caggatgcta cctccctgat ggaacaaacg
     4201 cgcgagctga agcagaaaat tgataccctt gaagcctcag ccacccaggc cgaagagcag
     4261 cttcgcagca ttcttcagcg catcccaaat ctgccgcacg ggtccgtccc cgcaggcagg
     4321 tctgagcatg acaacgtgga aatcaagcgc tggggcaaac agccggaatt tgccttccca
     4381 cccaaaccgc attgggagtt gggcgaggcc ctcggtattc tcgacctcga acgcgctgca
     4441 aaaatctccg gcgcgcgctt cgccgtctac tgggcagaag gcgcccgctt ggaacgcgcc
     4501 ctcatcaact tcatgctcga cgtgcacacg cgcgagcatg gctataccga agtgctgcct
     4561 cctttcatgg tcaacagcaa gtcgcttttc ggcaccggac agctgccaaa gtttgctgaa
     4621 gacctcttcc gttgtggaga cgagcaggga tataaagaag ccgagtaccg cgacagcgac
     4681 cactggctta ttcccacggc agaagtcccg cttacaaatc tttatcgcga tgaagtcctg
     4741 gatgaagcaa aactgactat ctcgctcacc gcctatactc cctgctttcg ctccgaggcc
     4801 ggatcttacg gaaaagacgt acgcggtatt atccgccagc atcagtttca gaaagtcgaa
     4861 ctggtcaagt tcgcaaagcc agaagagagc tacaacgaac tcgaaaaact gaccgcgaat
     4921 gctgaagcca ttctggaaaa actggaattg ccctatcgcc gcgtgctgct ctgtaccgga
     4981 gacatgggct tcggttcggc gaagacctac gatatcgaag tctggctccc aggacagggt
     5041 ctctatcgcg aaatctcttc ctgctcaaat acggagtcct tccaggcgcg ccgtgcaaac
     5101 atccgctacc gcccacaggg caaaaacaag gccgagtatg ttcacacact gaacggcagc
     5161 ggactcgctg taggccgcac ttggctggcc attctcgaaa actaccagca ggccgacggc
     5221 tccgtgcgcg tgccggacgc tctggttcct tacatgggtg ggcaaacagt aatcgctccc
     5281 agatgaaaca ttgttggtca tcccactctg cccctgaacg tggtgaaggg gcagtggaga
     5341 gacccgcatt tgccttacag ggctgaaaga aagcccacag caggtttagc aaagaaactc
     5401 cgagccacag taaaatgaag tttgccttat gggccgtatt ctcaaaagct atttcttctg
     5461 gacgtatgag cgcggcagct tccattacga cgtaatggtc acgctgatcc tcgctttcat
     5521 cttcgtcacg cctcaccttt ggaactacgg tgaccatccc gtgcctggga atttcgcgcc
     5581 cactgaagtt cttgtacgcg ccgacggacg cggcgggttc acttatgtcg ttcccgccga
     5641 ccaggtgcag ggcggctcat tgaacaccag gctcgaagcc gtcatcgagc gcatctctgg
     5701 ccccatggtt gtagaccact acgacactct cagggacgcc agcggtaaag tgaccgccta
     5761 caaagtctgg gcgcaccgat aataaacgca actgcatcta atagttccgt gtttctaact
     5821 ttgactatgc gcagattcaa gaccgtcttt ctgtccgccg ccttcgccgc cctcatcttt
     5881 gccgctgcct ctgcccactc gcaggacgac ctcagcaaag tcctcagcca gatggacgcc
     5941 gcctcccaga aattccagag cgcccaagcc gacttcaact gggaccagta cactgctgtc
     6001 gtccagagcc atgagacgca gaccgggacc atcgccttcc gccgttccgg caaaaacgtg
     6061 gagatggtcg ctcatgtgaa aaccgacaac ggccaacccg cgccgaagga catcctctat
     6121 aaaaacggtc agcttacctt ctatcagccg gccattaagc agcagaccat cttctc
//
LOCUS       DASRBV010000389         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_343580, whole genome shotgun sequence.
ACCESSION   DASRBV010000389 DASRBV010000000
VERSION     DASRBV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343580"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VFA02_14135"
     CDS             complement(<1..1294)
                     /locus_tag="VFA02_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZU11040.1"
                     /translation="MITRRTFMASAGLVLADSALRPMTKATSLDPSTLPAFAEPLPVP
                     AIAQFDGMRPSPDNPKEQIPYYQIAMREAMVKIHRDLPPTRMWTLGGTFPGPTIETQT
                     AKGMFVEWQNRLPARHFLPVDYSLHGAGKDVPEVRGVIHLHGGRTPPESDGYPEDWVV
                     PGKSQTCYYPSSQHAALLFYHDHTMGINRLNTYAGMMGMFLIRDPREDFLHLPRGRYE
                     IPLLICDRMLRADGQLEYPVSEKPGQVWVPEVFGNAILVNGKLLPYCDVEPRLYRFRV
                     MNGSNGRFFRFSLENGAEFYQIGSDQGLLVAPVKLKRVTMAPGERADLLIDFSSMSGE
                     QVNFVSDSFTVLQFRVRKQPAGERGVVPEKLHAFERLSEASAVKTRRLTLSENMDDVQ
                     RSMGMLLNNTPWHAPVTEKPVLNSTEIWELVNLTDDSHP"
     gene            complement(1365..>2600)
                     /locus_tag="VFA02_14140"
     CDS             complement(1365..>2600)
                     /locus_tag="VFA02_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU11041.1"
                     /translation="LMDAFPEYTGTPGPPPNGQTTNALVMGYYDGNTVTALWNYAQRY
                     AMNDNSYGTTFGPSTPGALNLIAGQTNGVSDQVNAGGDVIEDGAGGFTLISDADPLGD
                     VCSTTTGALVQMSGQNIGDLLNAKGITWGFFEGGFDLTRKNPNGTTGCNRSHTSAITN
                     VNKKDYIPHHQPFQYYKTTANPKHLRPSSVYAIGKTDQANHQYDLDDFYAAVKAGNMP
                     AVSFLKAAGYQDAHAGYSDPLDEQAFLVQVINFLETRPEWKTTAVVIAYDDSDGWYDH
                     QMGPIVNQSQTAADALNAPGQCGSSSATALAGVNADHVQGRCGYGPRLPLLVISPWAK
                     RNFVDHTTTDQSSVLRFIEDNWLGGQRITGSFDAIAGKLDGMFDFRAPRNRGLYLLDQ
                     NSGLVVVNQNGDHDPDWRW"
BASE COUNT          538 a          762 c          732 g          568 t
ORIGIN      
        1 tcgggtgcga atcatccgtc aggttcacca gctcccagat ttcggtcgaa ttcagcaccg
       61 gcttctctgt aaccggagca tgccagggcg tattgttcag cagcatgccc atggagcgct
      121 gcacatcgtc catgttctcg cttagggtca ggcggcgcgt cttcacagcg ctcgcttcag
      181 agagtcgctc aaaggcatgc agcttttccg ggactactcc gcgctctcct gccggctgtt
      241 tgcgcacgcg aaactgaaga acggtaaagc tgtcactgac gaagttcacc tgctcgccgg
      301 acatggaact gaaatcaatg agaaggtcag cgcgctcacc cggcgccatt gtcacccgct
      361 tgagttttac cggagcaaca agcagtccct ggtcggagcc gatctggtaa aactcggccc
      421 cattctccag tgaaaaccgg aagaagcgcc cgttcgagcc attcatgacc cgaaaacgat
      481 acagccgtgg ctccacatcg caatacggca gcagtttccc gttcaccaga atcgcgttgc
      541 cgaacacctc cgggacccac acctggccag gtttttctga tacgggatac tccagttgac
      601 catccgccct taacatgcgg tcgcagatga gcaggggtat ttcatacctg ccacgtggca
      661 ggtgtaagaa atcctcgcgc gggtcgcgga tgagaaacat ccccatcatg cccgcatatg
      721 tgttcagccg gttaataccc atggtgtggt catggtaaaa aagcagcgct gcgtgctggg
      781 atgaagggta atagcaggtc tgagatttgc ccgggaccac ccagtcttct ggataaccgt
      841 cgctttccgg aggcgtcctg ccaccgtgca gatgaattac gccgcgcacc tccggcacat
      901 cttttccagc gccgtgcagc gagtaatcca caggcagaaa atgcctcgca ggaagcctgt
      961 tttgccattc aacgaacatg ccctttgctg tctgcgtctc aatggtaggt ccgggaaatg
     1021 ttccacccag cgtccacatg cgcgttggag gcaagtcgcg atgtattttg accatcgctt
     1081 cgcgcatggc gatctggtaa tacgggatct gctctttcgg gttgtccgga ctcggacgca
     1141 ttccatcaaa ctgcgcaatg gccggaacag gcagcggctc ggcaaatgca ggcagcgtgg
     1201 aggggtccag cgatgtagcc ttcgtcatcg gccttaaggc cgaatcggcc agcacgagtc
     1261 cggcgctggc cataaacgtt cgtcgggtaa tcaaaattta ttcctttaaa ggaaaagggt
     1321 agcagctggc ggctgccacc ctcgatgaag gggatcacag tgagctacca gcgccagtcc
     1381 ggatcgtggt caccattctg attgaccaca accagaccgc tgttctggtc cagcagatac
     1441 aggccccggt tgcgtggcgc acgaaagtcg aacatgccgt ccagctttcc tgcaatcgcg
     1501 tcaaacgatc cggtgatgcg ctgcccgcca agccagttgt cctcaataaa gcgaaggacc
     1561 gagctttggt cggtggtggt atggtctacg aaattgcgct tcgcccaggg cgaaatcacc
     1621 agcagcggaa gccgcgggcc atagccgcag cgcccctgta catgatcggc attcacacct
     1681 gccagggcgg tggccgagct gctgccacac tgtcccggag cgttcagggc atcggccgcg
     1741 gtttgtgact ggttcacgat gggccccatt tggtggtcat accagccgtc cgaatcatca
     1801 taggcaatta caacagccgt cgttttccat tcaggccgcg tttcaaggaa gttgatgacc
     1861 tgcaccaaaa atgcctgctc atcaagcggg tccgaatatc cagcatgggc atcctggtag
     1921 ccagcggctt tcagaaagct cacagcgggc atgttcccgg cctttaccgc cgcatagaag
     1981 tcgtccaggt cgtactgatg gttcgcctgg tccgtcttgc cgatcgcgta cactgagctg
     2041 ggccgcaggt gcttcgggtt tgccgtcgtt ttgtagtact ggaacggctg atgatgagga
     2101 atatagtctt tcttgttcac attcgtaatg gccgatgtgt gggagcggtt gcagccggtt
     2161 gtgccattcg ggttcttcct ggtcagatca aatccgcctt caaagaagcc ccaggtgatg
     2221 cctttggcat tcaacagatc accgatgttc tgcccgctca tctgcaccag tgcaccagtc
     2281 gtcgttgagc aaacgtcacc aagagggtcg gcatcgctga tgagcgtgaa cccgcctgcg
     2341 ccgtcttcaa tcacatcgcc gccagcgttg acctggtcgc tgacaccgtt cgtctgtccg
     2401 gcaatcagat tgagtgcgcc cggcgtcgaa ggcccgaacg tggtgccgta ggagttgtca
     2461 ttcatcgcat agcgctgtgc atagttccag agcgccgtca cagtattgcc gtcgtagtag
     2521 cccatcacca gggcgttggt cgtctgcccg tttggcggag gacccggcgt tcccgtgtat
     2581 tcaggaaagg catccatcag
//
LOCUS       DASRBV010000390        19154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_442836, whole genome shotgun sequence.
ACCESSION   DASRBV010000390 DASRBV010000000
VERSION     DASRBV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442836"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..524)
                     /locus_tag="VFA02_14145"
     CDS             complement(174..524)
                     /locus_tag="VFA02_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU11042.1"
                     /translation="MESAFEIIAEPNRRAILGLLISSERSVGEIERQLQMSQPAVSKH
                     LRVLREAGFVESTVDAQRRLYRLRPEPLQEVDAWLAPFRQLWSARVDALERYLDRMEM
                     PVSTPPKKKTRRKR"
     gene            complement(622..2022)
                     /gene="dnaB"
                     /locus_tag="VFA02_14150"
     CDS             complement(622..2022)
                     /gene="dnaB"
                     /locus_tag="VFA02_14150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZU11043.1"
                     /translation="MATSPDIEFERGLPASPDSERSILGSVLLDNHLYNEAAEKLKSD
                     DFSLDSHRRIFARMAEMIDAGRAVDIITLSEELSKRKELEAIGGVAYLASLTEGLPRR
                     PSIEEYVRVVKDKSLLRQLINICSTAITQAADQSEDALEVLNAAESGLLEVTERGITR
                     GFASIPEIVRDSFGTIDNLYKEGREVTGLATHFTDFDRMTSGLQASELIIIAARPSMG
                     KTAWAINIAQNAAVYGGKVVAIFSLEMSKESLLRRMLASQALVDSQKIQKGFLLREDQ
                     QKLTHALEQLAESRLFIDDTPGISLTEMRAKARRLRQMQGGLDLVVVDYLQLMTASAP
                     GPGAKRYENRTQEVSAISRGLKALAKELQVPVIALSQLSRASEQRGGDKKPMLSDLRE
                     SGSIEQDADVVAFIHREAYYNRDENGNPDPESEGKAEIIIAKQRNGPTGSVHLAYLSK
                     CTRFENLAFGGDGGEY"
     gene            2204..2821
                     /locus_tag="VFA02_14155"
     CDS             2204..2821
                     /locus_tag="VFA02_14155"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU11044.1"
                     /translation="MFARLALLLAAFCACTSLLQAQSATVTQATANPAQNAEIKEMQK
                     LEDQWSDAVVKRDQYGLELLLSPLYVGISSNGEVTTRNQQIATLFQRSTDPLSMEQRV
                     VSVRMLGDVAVVNGTYIVRHRENGNPVEERGIFTHVFQRVRSNWLCINAQRTLVAEET
                     PGKQRTEKKKSDAELPFHIPFLHRGEEPASQPGQTAVQQDQQQPR"
     gene            2835..3377
                     /locus_tag="VFA02_14160"
     CDS             2835..3377
                     /locus_tag="VFA02_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZU11045.1"
                     /translation="MPFGQKPRKPKPLEEPALYEYAIGALARRMRTVAELKRLMRNRV
                     EEGEKGEAKISAVIAKLKDQRYLNDTNFATEYARLRQDNSAFGKRRVRQDLIQKGVHA
                     DVIARTLDSAYENVDEEALARQHLARKRVRKPANDKEAARVMRMLVRAGFSSGTIFKV
                     LKQWEVDDEALSGLDSIEED"
     gene            complement(3436..4989)
                     /locus_tag="VFA02_14165"
     CDS             complement(3436..4989)
                     /locus_tag="VFA02_14165"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU11046.1"
                     /translation="MSKFRWNDKVCLFALSLMFFALTQLCIFAQDAPSQSTASTTTGS
                     TPAQSTQGRGFWTRLGHAYLDDWTASSGNLPAAPDPPRRGTPAPLNSPPFPSADWPIG
                     GTVVIGAPDYQTYMLMQAINKNESRFKIYGWFDIGYNASTSNKGKYANAPAAYDAIPN
                     SVQLDQAALYFERLPDTVQKEHFDWGFRLASIYGMDYRFTTAYGVFSQQLLQKNNTYG
                     FDPVMYYLDFYLPHIGEGTDVRVGRYISLPDIEAQLAPNNYTYSHSLLYTYDCYTQHG
                     INVTTKINNHWTVQEGVSAGCEAAPWSKYAKLTGNACAAYTWSNGENNLYFCANSINS
                     GKYSYNNLSGYYLTYYHKFNATWHTDTEYWYQYERDVPNLCYGLDPCISYGPHANIGA
                     NSGSITSPALINGANGAQCNPAATHCYAPDWAIVNYIEHEWNNHHTSLTIRNEFFDDL
                     KGQRTGTATKYTEHLVGLNYWIGSTVTFRPELRLEHSYDKPAYDAPTGGAPTKSTQLT
                     FAGDLIWHF"
     gene            complement(5146..5535)
                     /locus_tag="VFA02_14170"
     CDS             complement(5146..5535)
                     /locus_tag="VFA02_14170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZU11047.1"
                     /translation="MPESTQYNHALDLCVSIYQLTSTFPAEETDGLSRQLRQAVVSLA
                     ARTAPGGEQKSAARGLLLEIEIMLRVADRLGFAASEKIAEVKNLCEKIAEKLADLNPP
                     AATVSQSGADPDKLPKRSSVGGIITPQ"
     gene            5610..6512
                     /locus_tag="VFA02_14175"
     CDS             5610..6512
                     /locus_tag="VFA02_14175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HZU11048.1"
                     /translation="MADSPARNTSPIKLIAIDIDGTLLPSAGMAISERNKRALRLAES
                     AGMEIVIATGRRQAYAVPLLEPLELKPDTVLITSNGTVTRTLAGEHIERALLPVETAR
                     GLCGVLRQFGGTAVFTFDRDGKGELVIESLEQLHTRIAAWVEANRPWIEEVRPLERAF
                     DAGEPPVQGMVCGTVEEMDRAEKWLASSPYAKLIEMHQTQYPKRNLSILDILPPGWSK
                     GVALRRLANRLGLQKEEIMAIGDNFNDLEMLEFAGNPVLMANASPELLAIGKQRGWRI
                     APANDDDGVAAILEETLCHVSVVE"
     gene            6626..7303
                     /locus_tag="VFA02_14180"
     CDS             6626..7303
                     /locus_tag="VFA02_14180"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZU11049.1"
                     /translation="MFIALCFRADAAEHITLKNGFDLICDHRQPDGDHVRLYLDSGSA
                     NFVDVAVNEIVSSEYVDLPKAPANTQPATANTKLTTADVHELLTRAGARHDLDVDLLA
                     SIVKAESGGNAHAVSRSGARGLMQLMPKTAAGLGVNDSFAAEQNVNGGTAYLDALLKK
                     YHDNLALALAAYNAGPAAVERWHGIPPYRETRAYVARVIHEFNRRYSQRISAQKQLAS
                     MQGSSGN"
     gene            7372..8439
                     /locus_tag="VFA02_14185"
     CDS             7372..8439
                     /locus_tag="VFA02_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZU11050.1"
                     /translation="MNKKNWLLWIVVLAALAVFALWARHRIHFQWHVFVEQLRLADWK
                     RISIAIGLIWMAYGLRALRWSIFLRPTKRVSPFALIGPMVIGFTGVALFGRLADLVRP
                     YLVSRRTQIPLSAQVAVYTVERMFDMGSMALIFSTVLLFSPDRHSLPRPDLINKAALG
                     GLLVAVFLALFTAFVRASGKAVARLLGRLLGALSQNLGHQVEDKILNFRDGLNAIGSM
                     GDLLLVVIISLVHWSMITLAYLETTRAFVADPILSHMRLSQCMLLMAASMGSSLITLP
                     VLGWFTQIGLTTAAMQAFFRVSWEPALGCGSVLLLVTFLSVIPLGLAWARFEQVSLRK
                     VARESEHAGEEIAAQHEQAQA"
     gene            8462..9082
                     /gene="rdgB"
                     /locus_tag="VFA02_14190"
     CDS             8462..9082
                     /gene="rdgB"
                     /locus_tag="VFA02_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZU11051.1"
                     /translation="MHQTLYVASGNPGKLRDFAVAAELFRITIAPLPNLTDIPAPEEN
                     GTTFEENARIKAVYYSHFAPGRFIIADDSGLEVDALGGAPGVRSARYAEDTGFEAPGS
                     TDERNNLCLLQNLSSRKAGKNTARYRCVIALARDEQVLLTAEGSVEGEVLPAPRGTGG
                     FGYDPLFYLPHLRRTMAEIDLPQKQKLSHRGKALLKLLEQLTQRPI"
     gene            9201..12470
                     /locus_tag="VFA02_14195"
     CDS             9201..12470
                     /locus_tag="VFA02_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU11052.1"
                     /translation="MRRLSLSILAIAVAFTCSARPVFAQFSGSITGEVLDPSGSVVPG
                     ATLVLTRTDTGEKHTATTNASGVYLFVSLAPGAYQLTASASGFGETHNNLVLQTGQVL
                     NFPVRLAVAGATQTVQVTTETPVLDTADTRLQETLSTQTLSTLPLSGRNMISLVTLAP
                     GVTGTGVTSNGSPGSGRDNYSTETQVDASANGQGAVGNMYVIDGLDVTSSIRPGVLNM
                     TPNPDSIAETSIQTNTYNVDYGRASSIQMTMTTKSGGNSYHGNASDYFTYQNFFAGTE
                     FTHKYAPFHSNNISATIGGPIVPRRNAGFFFFSIEPLRSSAAVSSTTSFEDPAFTAFA
                     QSAFPNTIGTKLLTSYPVSRVANVTVSSTAASLYPATCGTPATNNLPCSMAIIDTGNF
                     TAAQYRNGLQYNFRLDKDFASDRLYGTFYRTTLNTNGVSPRSAFDTSSTFSQYAIQVN
                     ETHTFSPDTLNEAAFAAMRVEGIQPQKGLFSVPVVNVTGIGTGFGAGFAQGDFIQHNY
                     HWRDVLTHTYKSHVLRFGYEGLFGDDVEIFNGPYDQPTFQFNGLLDLAQDNVYTETGL
                     AYSPLSGQRDQWNWNAAGVTHGVFAEDTWKATPRLTLNFGLRWDDFGNPWSRSQNTAF
                     SNFYYGPGQSLQEQIANGILVRHHHALNRSITDVFSPRGGVAWDVAGDGKWLIKGGAG
                     FFHNWPTLANVQEEFRGNPPGDIFPTFYGGQTPAPVFGFGTSNQKPFNFPAPSIAAYS
                     LNAKGGINGLQFGVGGIDPNLRSPVAYVYSASLEHQLLQNLVAGISYSGSKGRDLLSG
                     GGQVYNVSYGQDINALPGDLIIHNSTTPTRLNTSFGAVSYTQNDRLSDYNALILSLRG
                     RFSRAFFTASYTYSSSKDDTQVYPTYINPHQWYGPSVWDAPNRFSLAWNYEFPAYNSG
                     LGLLGRVASGWQLSGTTIIQDGNPFTVSTNAAFRPLTDSSGKFTGYAPGSGDFNADGD
                     NFDFPDVVNYHYSTGRHNYLQGLFAPGNFPAPSTFGSEGNERYSAFRGPGFAQWDTAL
                     LKKTAITEAVSLQLRFEFYNLFNRPNLTGMDTNLPDANFGKATNQYTPRFLQIGGNIV
                     F"
     gene            12525..13934
                     /locus_tag="VFA02_14200"
     CDS             12525..13934
                     /locus_tag="VFA02_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU11053.1"
                     /translation="MLLTVPAMPQADTQFAVAMRDMQQRNFSAALHSANDALKAAPRD
                     CRWLTVKGMALDNLGERQNALAFFQQAVNNCPRFLPALEGLAQIQYAQHSPQAAHTLE
                     TILTLRPDDETTHAMLAVLDWQKGDCQHAVEHFAKGGRQVDANPAAQTEYGFCLLQTG
                     DTQHAEDVFQHLMEQSNSPQDRMRFAFAAWKNKDHTRALDALAPLISATAPDAMALRL
                     AAQIAEDKGDTPDAVKWLRQAIVADPKNAEGYLIFAGISYTHQSWPVGIDMLNAGLTQ
                     LPNDARLYLARGVLRVQLSQLDLAIADFESAHRLDPKLSLAEDAIGMMQSQKHQNAAA
                     LETFRNQAMQHKDDALLQYLYAEALSQQDNTGPQQTAEAIAALRRAVAIEPDYRPAHD
                     LLSMLYLRANDLPLAISHAERALQLDPNDDTAVYQELMAYRRLGKKEQVDALVARLKE
                     IKQTQQNAKTKYLLQEAQP"
     gene            14040..15800
                     /locus_tag="VFA02_14205"
     CDS             14040..15800
                     /locus_tag="VFA02_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZU11054.1"
                     /translation="MFAAGTDAIVPQLSQFNYGDVQLLDGPMLDQFNANHAFFLAIDE
                     DKLLKPFRKRAGLPAPGEDMGGWYNFSDDFDPPRNMTGFVPGHSFGQYLSSLARAYAV
                     TGSKPTQEKVHRLVRGFAATVSEKFYVDYPLPAYTFDKINCGLMDAHQFAADPIALSV
                     LNKETDAVLPFLPEKALNRREQAQRPHKNIAFTWDETYTLPENLYLAYKRGAGGRYKQ
                     LAARFLEDDTYFDPLAEGQNVLPGEHAYSHVNAFSSAMQAYLVTGNEKYLRAARNGFE
                     FVRTTQSFATGGWGPDETFRSPDSGEMGESLTRTHASFETPCGAYGHFKITRYLLRVT
                     GDSRYGDSMERVLYNTILGARPLLEDGTSFYYSDYNNNASKFYHQDKWPCCSGTFPQI
                     TADYGISSYFRHPHGIYVNLYVPSKVSWTQNGARCELRQRTSYPLVPETEMEVNPERP
                     AKFVVAVRIPSWAGPKTSVAINGKRIGPEIRPGTFFPVEREWKKGDRIEIFFDMPGVL
                     EAVDSKHPNTVALVHGPLALFAIEPLNVQMKRAQLLSAAQLSRGSDHWIVESDAGRIT
                     MKPFASISNEKYRLYHDVNA"
     gene            complement(15866..19150)
                     /gene="carB"
                     /locus_tag="VFA02_14210"
     CDS             complement(15866..19150)
                     /gene="carB"
                     /locus_tag="VFA02_14210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZU11055.1"
                     /translation="MPRRNDITKILVIGSGPIVIGQSAEFDYSGTQACKALKQEGYEV
                     VLVNSNPATIMTDPELADRTYVEPLTREYVEEIIRLESQMLKDEGRKGQFALLPTVGG
                     QTALNLAVELADSGVLEKYNVELIGAKLDAIKKAEDRLLFKDAMTRIGLDVPKSALVN
                     NIRDGLEFVTKIGFPAIIRPSFTLGGSGGGIAYNREELMEILARGLDLSPVHECLIEE
                     SVLGWKEYELEVMRDLADNVIIVCSIENMDPMGVHTGDSITIAPAQTLTDREYQKMRT
                     AAIAVMREIGVETGGSNVQFAVNPLTGRMTVIEMNPRVSRSSALASKATGFPIAKIAA
                     KLAVGYTLDEIPNDITKMTPACFEPTIDYVVTKIPKWQFEKFPGADENLGPQMKSVGE
                     VMAIGRTFKESLMKALRSLDTGKKVGAEILDPRRLTQMLVTPKPGRIDYIRFAFRRGL
                     SVREVARMTSIDPWFLYHIKEVTDMIASLADYNPDTVSAETLRKAKRMGISDERIAEV
                     WGLSGSEGVKQVRQLRNRHGIQPVYKLVDTCAAEFESMTPYLYSCYDEEDEAIPTSKH
                     KVIILGAGPNRIGQGIEFDYCCCHAAFALKEDGYETIMVNCNPETVSTDYDTSDRLYF
                     EPLTLEDVLAVYEHEAKSGAEIGMIVQFGGQTPLNLALRLKEAGVPILGTSPESIDLA
                     EDRKRFGKLLEELNIPQPPGAMATSVEEALAGAERIGYPVLVRPSYVLGGRAMTIAFD
                     SDAVARYMKEAVEYSQERPVLIDHFLEDAVEVDVDALCDGTDVVIAGIMQHIEEAGIH
                     SGDSSCVLPPVDLSAEVLNTIRTYTRKLALALNVVGLVNLQFAIQRDKVYVIEVNPRA
                     SRTVPYVSKATGVPLAKIASRLMTGRRLRDFLSEYVASGRDLETGTHYFVKSPVFPWN
                     KFPGVDTVLGPEMKSTGEVMGVADNFGEAFAKAQLSAGQHLPLKGTVFFSVNDRDKAQ
                     VVDVARQYVELGFHLVATEGTAQVLEQAGMVVERVYKVKEGRPNVVDLIKGDRIQLIV
                     NTPHGQDPFFDEKAIRRAAVMQRIPTITTLAAARAAAEGIQARQKHTTTVNALQQLHA
                     AQAVAK"
BASE COUNT         4347 a         5567 c         4995 g         4245 t
ORIGIN      
        1 ccggtccact tcaaatgggg cccattcgcg cagatgcgcc ggatcagtca gtgccagcca
       61 gaccttttcc ggcgggtgcc gcagttctct tgtaagaatg agcgtccact tgccttcgcc
      121 cttttgcacc tgtgctccgc cggcctcacc cggcacatat tcttcacgcc tggtcatcgt
      181 tttctccgtg tcttcttttt ggggggtgtg gataccggca tctccatgcg gtcaagatag
      241 cgttcgagcg cgtccacacg ggccgaccac aactgccgaa acggggccag ccacgcatcc
      301 acttcctgaa gcggttctgg cctgagccgg tagagacggc gttgcgcatc cacagtggac
      361 tccacaaaac cggcctcgcg cagcactcgc agatgcttgg ataccgccgg ctgcgacatc
      421 tgcaattgac gctcaatctc gcctacagac cgctcggacg aaatcagaag acccagaatc
      481 gcccggcggt tcggctctgc aatgatttca aaagcagatt ccatgtcaat ttatatactt
      541 tattcgttat ataactgtca aggcatatac atgagcgaaa acagacagaa tcgcagaaag
      601 gacagggaag atgacggaga atcagtattc tccgccatcc cctccgaatg ccaggttttc
      661 aaagcgggtg catttcgaga gataggccag atgcactgaa ccggtcggac cattacgctg
      721 ctttgcaatg atgatttcgg ccttgccttc gctctctgga tcaggattac cattctcatc
      781 gcggttgtaa tacgcctcgc gatggataaa ggcaacaacg tcagcgtctt gctcaatcga
      841 gccggattcg cgcaggtcag agagcatcgg ctttttatcg ccgccgcgct gctctgaggc
      901 gcggctcaac tgcgaaagcg caatcaccgg gacctgtaac tcctttgcca gcgccttcaa
      961 cccgcgggaa attgccgaga cttcctgcgt gcggttctca taacgcttcg ctcccggacc
     1021 aggcgcactt gccgtcatca attgcaggta gtccactacc accaggtcca gcccgccctg
     1081 catctgccgc agccgccggg cttttgcgcg catctcggtc agcgaaatgc cgggcgtgtc
     1141 atcaatgaac aacctcgatt ctgcaagctg ttccagtgca tgcgtcaatt tctgctggtc
     1201 ttcacgcagc agaaagcctt tctgtatctt ctgcgaatcc accaacgcct gcgaggccag
     1261 catacggcgc agcagcgact ccttcgacat ttcgagcgaa aagatggcga ccactttccc
     1321 gccatagacc gccgcgttct gagcaatgtt gatggcccac gccgtttttc ccatcgaagg
     1381 acgcgccgca ataatgatca gttcggaagc ctgcaggccg ctggtcatcc ggtcgaaatc
     1441 cgtaaaatgc gtggcgagtc cagtgacctc gcggccctct ttatagaggt tgtcaatggt
     1501 cccgaatgag tcgcggacaa tctccggaat gctggcaaac ccgcgcgtaa tgccgcgttc
     1561 agtgacttcc agcagcccgc tctcggcggc attcaggacc tcaagcgcgt cttcgctttg
     1621 atccgccgcc tgggtaatcg cggtggagca gatattgata agttgccgca ggagtgactt
     1681 gtcttttaca acgcggacat actcttcaat cgaaggccgc cttggcagtc cttctgtgag
     1741 cgacgcaagg taggccacgc caccgatggc ctccagttcc ttgcgcttgc tcaactcttc
     1801 tgagagtgtg atgatgtcca cggcacggcc cgcatcaatc atctctgcca tgcgcgcaaa
     1861 gatccgccgg tgcgaatcga gcgagaaatc atcagacttc agcttttccg cggcttcgtt
     1921 gtaaagatga ttatccagaa ggacagagcc gagaatagaa cgctcagaat caggacttgc
     1981 cggaagcccc cgctcaaatt cgatatcagg agaagtagcc atgactctta ttaagtgtag
     2041 ggtttttacg gcggcgcagg cgcctcagac cgaaaatgtt cctgtgaatg ccataggaaa
     2101 tttcggaaaa cgtggatttg agcgttgcat cccgtgcctt cctttcgtct aatccctcaa
     2161 aggcgatatc atcaaaacag cccccggata tcttatgaaa ccgatgtttg cccgtcttgc
     2221 cctcttgctg gccgcattct gtgcctgcac ttccctgctt caggcccagt ccgctacagt
     2281 gacgcaggcc acggccaatc ccgcccaaaa cgctgaaatc aaagagatgc agaagcttga
     2341 agaccaatgg agcgatgccg tcgtcaagcg cgaccagtac gggctggaac tgctgctttc
     2401 acccctctac gttggcattt catccaatgg cgaagtcaca acgcgcaacc agcagatcgc
     2461 cacgcttttc cagcggagca cggaccctct cagcatggaa cagcgcgtgg tcagcgtccg
     2521 gatgctgggc gacgtggcgg tcgtaaacgg aacttatatc gtgcgccacc gggagaatgg
     2581 caatcctgtc gaagaacggg gcatctttac acacgtcttc cagcgcgtgc gctctaactg
     2641 gctctgcatt aacgcgcagc ggacgttggt agcagaggaa acccccggga aacagcggac
     2701 agagaagaaa aagagtgacg cggaactgcc attccacatc cctttccttc acaggggtga
     2761 ggaacctgca tcacaacccg ggcaaactgc cgttcaacaa gaccagcagc agccgcggta
     2821 acggaaggca agatatgcct ttcggtcaaa agccccgcaa accaaagccg ctcgaagaac
     2881 cagcgcttta cgaatatgcc atcggtgcgc tggcccgccg catgcgtacc gttgccgaac
     2941 tcaagcgatt gatgcgcaat cgtgtcgaag agggcgaaaa aggcgaagca aagatcagcg
     3001 cggtcatcgc aaagctgaaa gaccagcgtt atctcaacga cacaaatttt gctacagaat
     3061 acgcccgcct gcgccaggat aactccgcct ttggcaaacg ccgcgtacgt caggatttga
     3121 tacaaaaagg cgttcacgcc gatgtgattg cacgcacact cgactctgct tacgaaaacg
     3181 tggacgaaga agcccttgcc cgtcagcacc ttgcccgcaa gcgcgtccgc aagcctgcga
     3241 acgacaaaga ggctgcgcgt gtgatgcgga tgctcgtgcg ggcagggttt tcatccggga
     3301 cgattttcaa agtcctgaag caatgggaag ttgacgatga ggcgctctcc ggactcgatt
     3361 cgatcgaaga agactgagcg cgcacccttt cgcgttccat gccaaagaat gcgacgccct
     3421 ggtctattgc cactcttaaa aatgccagat caggtccccg gcaaacgtca gttgtgttga
     3481 tttcgtagga gcgccgccgg ttggagcatc atacgctggc ttatcatacg agtgctccag
     3541 ccgcagttca ggccgaaacg taacagtact gccaatccaa tagttcagcc ccacgagatg
     3601 ttcggtgtac ttcgtcgctg ttcccgtgcg ctggcctttc aaatcatcga agaattcatt
     3661 gcgaatggta agcgacgtat gatggttgtt ccattcgtgc tcaatatagt tcacaatggc
     3721 ccagtccgga gcgtagcagt gtgttgccgc cggattacac tgcgccccat tggccccgtt
     3781 tatcagcgca ggtgaagtga tgctgccgct gttggcgccg atatttgcat ggggcccata
     3841 actgatacac ggatctaacc cataacatag gttaggcacg tcgcgttcgt actgatacca
     3901 gtattcggtg tctgtatgcc aggtagcatt aaatttgtga taatacgtca gataataacc
     3961 agacagattg ttataagagt acttgccgga gttgatcgaa ttcgcgcaga aatataagtt
     4021 gttttcgccg ttgctccacg tataggccgc acaggcgtta ccggtaagct ttgcatactt
     4081 gctccacgga gcagcttcgc atcccgccga aacaccttcc tgcaccgtcc agtgattatt
     4141 gatcttggtg gttacattga tgccgtgctg cgtatagcaa tcgtaggtgt aaagcagcga
     4201 atgactatag gtgtaattgt ttggggccag ttgcgcttca atgtctggca gagagatgta
     4261 gcgccctacg cgaacatctg ttccttcgcc aatgtgcggc agataaaaat ccaggtaata
     4321 catcactgga tcaaagccat aagtattgtt cttctggagc aattgctgac tgaagacgcc
     4381 atatgctgtc gtaaagcggt aatccatccc ataaatgctg gccaggcgaa aaccccagtc
     4441 aaaatgctct ttctgcacag tgtccggcag ccgttcaaaa tagagtgccg cctgatcaag
     4501 ctgcactgaa tttggaatcg cgtcataagc tgccggagca ttggcatact tgcccttgtt
     4561 cgacgtgctg gcgttatagc caatatcaaa ccacccataa atcttgaagc ggctttcgtt
     4621 tttgttgatg gcctgcatga gcatataagt ctggtagtca ggcgcgccaa tcacaaccgt
     4681 gccgccaatc ggccagtctg ctgaagggaa cgggggagaa ttcagcggag cgggtgttcc
     4741 cctgcgcggc ggatcaggtg cagcaggaag attgccggag cttgccgtcc agtcgtcaag
     4801 ataagcgtgg cccagacgcg tccagaatcc gcggccctgt gtggactgcg ctggtgtgct
     4861 gccggtagta gtggatgcag tgctctgaga gggcgcgtcc tgagcaaaaa tacagagttg
     4921 cgtcagggca aaaaacatca ggctcaaagc aaaaagacaa actttatcgt tccatcgaaa
     4981 cttgctcatg ggtgtccgtt cgattgccta aaacaggctg atttgcaatc taacggacca
     5041 tccataagcc ccggataagt gagccataag gtgttcgtaa aggtccttta tgggggcgta
     5101 aggaaaggag ctgcctgcaa cagatctctc cgaccagacc tggttttact gtggagtgat
     5161 aattccaccc accgaactgc gctttgggag cttgtctgga tctgccccgg attgagagac
     5221 ggttgcagca ggcggattca ggtcagccag cttttctgca attttctcac agagattttt
     5281 gacctcagca atcttttccg aagcagcaaa acctagcctg tcagccacac gaagcatgat
     5341 ttcaatttcc agcaaaaggc cccgggcagc gcttttttgc tccccgcctg gcgcagttct
     5401 ggcagccagc gataccaccg cctggcgaag ctggcgcgac aatccgtctg tctcctcggc
     5461 tggaaaagtg ctggtcaact gatagatact cacacacaga tccaaagcat ggttgtattg
     5521 tgtactttcc ggcaactcat tctcctctgt tccatccctg aacagcattt tagagtgtcg
     5581 gcgcctgtcg tccctgtaaa ctataaggaa tggccgattc tcccgcccgg aacacctctc
     5641 caatcaaact cattgccata gacattgacg gtacactgct gccttcggct ggaatggcca
     5701 tcagtgagcg aaataagcgc gcactgcgtc tggcagagag tgcagggatg gaaatcgtca
     5761 tcgctacagg cagacgccag gcctatgccg tgcctctgct tgagccgctg gaactgaagc
     5821 cggatacagt cctgattacc tccaatggca ccgttacgcg cacccttgcc ggcgagcaca
     5881 ttgagcgggc gctgcttcct gtcgagaccg cgcgcggact ttgcggcgtt ttacggcagt
     5941 ttggaggaac ggccgtcttc accttcgacc gcgatggtaa aggcgagttg gtcatcgaat
     6001 cgctggagca gctccacacc cgcattgccg catgggtcga ggcaaaccgt ccatggatcg
     6061 aggaggtccg cccgctggag cgcgctttcg atgcgggtga accgcccgtg cagggaatgg
     6121 tttgcggcac tgtagaggag atggaccgtg ctgaaaagtg gcttgcctcc agcccctatg
     6181 caaagctcat agagatgcat cagacccagt atccaaaacg caacctcagt attctggaca
     6241 tcctgccacc gggatggtca aaaggcgtgg ccctgcgcag gctcgccaac cgcctcggcc
     6301 tgcaaaaaga agaaattatg gcgattggcg acaattttaa tgacctcgaa atgctcgaat
     6361 ttgctggaaa tccagtactt atggccaatg cctcgccgga gttgctggca ataggaaagc
     6421 agcgcggatg gcgcatcgct cctgcgaacg atgatgacgg cgttgcggca atacttgaag
     6481 aaaccctttg tcatgtgagc gtggtagagt gaccttcatg cggcattttg ggcaactctg
     6541 ccagcgcgtt tcaggcacag aggacgcaaa gtattcgagg tgttccccgg catacaatac
     6601 ttttcggacc ttcgcagcag cgctgttgtt tattgctctc tgttttcggg ccgatgccgc
     6661 agaacatatc actctgaaaa atggctttga tctgatctgc gaccatcgcc agcccgacgg
     6721 cgatcatgtc cggctctatc tcgattccgg cagcgcgaac tttgttgatg tggccgtaaa
     6781 tgaaattgtc agttcagagt acgtggacct cccgaaggcg ccagcgaaca cacaaccggc
     6841 aacggcaaac acaaagctga ctacagccga tgttcacgag cttctgacgc gcgccggggc
     6901 ccggcacgat ctggacgtag acctgcttgc aagcatcgta aaagcagaga gcggcggaaa
     6961 tgcccatgcg gtaagccgct ccggagcacg tgggctgatg cagcttatgc cgaagacggc
     7021 ggcagggctc ggcgtgaatg acagctttgc tgctgagcag aatgtgaacg gcggcacggc
     7081 ttatcttgat gcgctgctga agaaatatca tgacaatctt gcactggccc ttgcggcata
     7141 taacgccgga cccgcagccg tggaacgctg gcacggaatt ccaccttacc gggaaactcg
     7201 ggcctatgta gcacgagtga tccatgaatt caaccgcagg tattctcagc gaatatcggc
     7261 ccaaaagcag ctggcgagca tgcaaggctc atccggcaat tgattcccat cctgcgcagc
     7321 cgggtgctcc atacaagtcc ggagttgggt gtggaacagt aagggtatct cttgaataag
     7381 aagaactggc ttctgtggat tgtggtactg gccgcacttg cagtttttgc gctgtgggcc
     7441 cggcaccgca tccattttca atggcatgta tttgttgaac agctcaggct ggctgactgg
     7501 aagcgcatca gcatcgccat cggtctgatc tggatggcct atggtctgcg cgctctacgc
     7561 tggtccatct ttctccggcc caccaaacgc gtttctcctt ttgctctgat tggccctatg
     7621 gtcatcgggt tcacaggtgt cgcgctcttc gggagactcg ctgaccttgt gcgcccttat
     7681 ctggtctcgc ggcgcacgca gattccgctc agcgcgcaag tcgccgtcta cactgtggag
     7741 cgcatgttcg atatgggctc catggcgctt atcttttcca ctgttctgct gttttctcca
     7801 gaccgccatt ccctgccccg gcccgacctc atcaacaaag ccgcactggg aggcctgctt
     7861 gtggccgtct ttctggcatt gtttacggcc tttgtacgcg cttcaggaaa agctgtcgcg
     7921 cggctgctgg ggcggctcct gggagcactc tcgcaaaacc tgggccatca agtcgaagat
     7981 aaaattctca atttccgtga cgggctgaac gccatcggct ccatgggcga tcttctcctc
     8041 gtagtcatca tctcgctcgt ccactggagc atgattactt tggcatatct tgagacaacg
     8101 cgcgcctttg ttgccgaccc cattctctcg cacatgcgcc tttcccaatg catgttgctg
     8161 atggcggcca gcatgggcag ctcccttatc actcttcccg tgcttggatg gtttacacaa
     8221 atcggcctga ccacagcagc catgcaggcc ttcttcagag tgtcgtggga accggcgctc
     8281 ggctgcgggt ctgttctgct gctggtgaca ttcctcagcg taattcccct gggactcgca
     8341 tgggcgcgct ttgagcaggt ttctctacga aaagtcgcgc gcgaaagcga acatgctggt
     8401 gaagaaatcg cggcccagca cgaacaggca caagcgtaaa acgaacctgt acgctgtata
     8461 tatgcaccaa acgctctatg ttgcttcagg caatccggga aagctccgcg attttgctgt
     8521 cgctgccgaa ctctttcgta tcaccatcgc tccgctgcca aaccttacag acattcccgc
     8581 tccagaggaa aacggcacta cctttgaaga aaacgcgcgc atcaaggccg tatactacag
     8641 ccattttgct cccggccgtt tcatcatagc cgatgattcg ggcctggaag tagatgcgct
     8701 cggcggcgct cccggtgtgc gctccgcgcg ctatgcagag gacactggat tcgaagctcc
     8761 cggatccaca gacgagcgta acaacctctg cctgttgcaa aatctttctt cacgcaaggc
     8821 cggaaaaaat acggcgcgtt atcgttgcgt aatcgccctt gcccgcgatg aacaggtcct
     8881 gctcacggcc gaaggttcag tggaaggaga agtcctcccg gcaccgcggg gcacaggtgg
     8941 gttcggctac gacccgttgt tttatctgcc tcatttgcgc aggacgatgg cagaaataga
     9001 cctgcctcag aaacaaaagc tgagccatcg tggaaaagcg cttttgaaat tgctggaaca
     9061 actgacccaa aggccgattt gatagcgtcg cttatcagtg gaaaatgcaa ttgacaatgt
     9121 atgagcggtt cgactacatt aatcgccgtt gagaaaacgt tttccatcaa gagactcaaa
     9181 aatatgagga ggttagatgc atgagacgcc tgtccctctc cattcttgcg atcgccgttg
     9241 cctttacttg ctctgcgaga ccggttttcg ctcaattcag tggatccatc accggagaag
     9301 tcctcgaccc ctccggatca gtcgtgcctg gcgcaaccct ggtcctgaca aggactgata
     9361 ccggagaaaa acacactgca accaccaatg cttccggcgt ctatctgttc gtcagccttg
     9421 cccccggcgc ttatcaatta actgcaagtg cttcgggctt tggtgaaacg cacaataacc
     9481 tcgtcctgca gacaggtcag gtacttaatt ttcctgttag actggccgtt gctggcgcga
     9541 cccagaccgt tcaggtcact accgaaacac ctgttctgga taccgcagat acgcgcctgc
     9601 aggaaacgtt gtctacacaa accctgtcca cgctccctct ctccggacgc aacatgatct
     9661 ccctggttac gcttgctccc ggagtgaccg ggactggtgt caccagcaac ggcagtcccg
     9721 gatcaggccg cgacaattac tcgactgaaa cacaagtaga tgccagcgca aacggacaag
     9781 gcgcggtggg caatatgtat gtaatagatg gcctggatgt aaccagttcg attcgacctg
     9841 gcgttctgaa catgacgccg aatccggatt cgattgccga aaccagcatt cagaccaaca
     9901 catacaacgt ggattatggg cgcgccagtt ctatccagat gaccatgacg acaaagtcgg
     9961 gaggcaactc ctatcacggc aacgcgagcg actacttcac ctatcaaaac ttttttgcgg
    10021 gcactgaatt cacccataag tacgcgccgt tccatagcaa taacatctca gctaccatcg
    10081 gcggccccat cgttcctcgt cgtaacgctg gtttcttttt ctttagtatt gagccgttgc
    10141 gctcgtcggc ggcggtaagt tcgactacgt cttttgaaga tccggcattt accgcatttg
    10201 cgcaatcagc atttccgaac actattggca caaagttatt aacaagctat cccgtcagcc
    10261 gcgttgcaaa tgtgacggta tcttcaaccg cagcatctct ttacccagcc acctgcggaa
    10321 caccagccac aaacaatctg ccctgcagca tggcgatcat tgataccggg aatttcaccg
    10381 ccgcgcaata tcgcaacgga ttgcagtaca acttccgcct cgacaaggat tttgccagtg
    10441 accggctcta tggcaccttc tacaggacca ctctgaatac gaatggcgta agtccacgat
    10501 cagcattcga tacatccagc acgttcagtc agtatgccat tcaggtgaat gaaacacaca
    10561 ccttcagtcc ggatactctg aacgaagctg cttttgctgc gatgcgtgtc gagggcatcc
    10621 agccgcagaa aggtctcttc agtgttcctg tggtgaacgt cactggaatt ggcacgggct
    10681 tcggagcagg gttcgcgcag ggcgatttca ttcagcataa ctaccactgg cgcgatgtac
    10741 tgacgcacac ctacaaatca cacgttctaa ggtttggata cgaaggcttg tttggggatg
    10801 acgttgaaat cttcaatggc ccgtatgacc agccaacttt tcaatttaat ggcctgctcg
    10861 acctggcaca ggacaacgtc tataccgaaa cagggctggc ttatagtcca ctgtctggcc
    10921 agagagacca atggaactgg aatgcggcag gcgtaacgca cggtgtattc gctgaagata
    10981 cctggaaggc cactcccagg ctcacactaa acttcggcct ccgttgggac gacttcggaa
    11041 atccctggtc gcgcagccag aacacggcct tctctaactt ctactacggc ccaggtcaat
    11101 cattgcagga acagattgcc aacggcatcc ttgttcggca ccaccatgcc ctcaaccgtt
    11161 ccatcacaga tgtcttcagt cctcgcggag gcgttgcatg ggacgtagca ggcgatggaa
    11221 aatggctgat caaaggcggg gctggcttct ttcacaactg gccgactctc gctaacgttc
    11281 aggaggagtt ccgcggaaat cctccaggag atatcttccc caccttctat ggcggccaga
    11341 caccggcacc cgtttttggc tttgggacct ccaaccagaa gcctttcaac tttcccgctc
    11401 cctcgattgc agcttattcc ctcaatgcaa agggaggaat caacgggttg caatttggag
    11461 ttggcggcat agatcctaac ttgcgctctc ctgtggctta tgtgtattca gcctcattag
    11521 agcaccagct cttgcaaaat cttgtggccg gaatctccta ttccggatcg aagggcagag
    11581 atcttctatc aggaggaggt caggtctata acgtgagtta tgggcaggac atcaatgctc
    11641 ttcccggcga tctcatcatc cacaacagca cgaccccaac gcgcctgaat accagcttcg
    11701 gcgcagtcag ttatacacag aacgatcgtc tttctgacta caacgcgctg attctttccc
    11761 tgcgagggcg cttctcgcgg gccttcttta ccgcttccta cacctattca tcttccaaag
    11821 atgacacgca ggtctatccc acctatatca atccgcatca gtggtatggg ccatctgtat
    11881 gggacgcgcc caaccgtttt tcgctggcct ggaactatga gtttccggct tacaacagcg
    11941 ggcttggtct cctgggccgc gttgccagcg gatggcaatt gagcggcaca accattattc
    12001 aggacggcaa tccgtttacc gtatccacaa acgccgcctt caggccgttg acggacagca
    12061 gcggcaagtt cactggatat gcgcccggaa gcggcgattt caacgcagac ggcgacaact
    12121 tcgattttcc tgacgttgtg aactaccact attcgactgg gcggcataac taccttcagg
    12181 gattgttcgc tcccggtaac tttcctgcgc catccacatt tggctccgaa ggcaatgaac
    12241 ggtacagcgc attccgcggc cccggctttg ctcaatggga taccgctctg ctgaaaaaga
    12301 cggccatcac agaagcagtc tctctgcaat tgcggtttga gttttacaac ctcttcaaca
    12361 gaccaaacct tacgggtatg gacacaaacc tgccagacgc gaatttcggt aaagcaacaa
    12421 atcaatacac gccccggttc ctgcagattg gaggaaacat cgtcttttag aatgcaatcg
    12481 tggccttgtg gaacaaatat tgcttctatg gaatggctgc ctgtatgttg ctgacggtcc
    12541 cggcaatgcc acaggcagac acccagttcg ccgttgcgat gcgcgacatg cagcagcgaa
    12601 atttctctgc ggcgctgcat tcagccaatg atgcgctaaa ggcagcgcca cgcgattgcc
    12661 gctggcttac ggtaaagggt atggcactcg acaatcttgg tgagcgacag aatgcgcttg
    12721 cattctttca gcaggcggtc aacaattgtc cgcgctttct gccagcgctc gaaggcctgg
    12781 cgcagatcca gtacgcccag cattcaccac aggcagcgca cacactggaa accattctca
    12841 ctctgcgccc tgacgacgaa accacgcatg ccatgctggc tgttctggac tggcaaaagg
    12901 gcgactgcca gcacgctgtc gagcattttg caaaaggagg gcggcaggtg gacgccaatc
    12961 cggcggcaca gacagaatat ggcttctgcc tgcttcaaac cggcgatacg cagcacgcag
    13021 aagacgtctt tcagcatttg atggagcaga gcaacagtcc acaagaccgc atgaggttcg
    13081 cattcgcggc atggaaaaac aaagaccaca cccgcgcgct ggatgcgctg gccccattga
    13141 tttcagccac agcccctgac gcaatggccc tgcggctcgc tgcacagatt gctgaagaca
    13201 agggtgacac tccggacgca gtcaaatggc tgagacaggc gatcgtagct gatcccaaaa
    13261 atgccgaagg ctacctgatt tttgccggca tttcctatac acaccagtcc tggccagtcg
    13321 gcattgatat gctgaacgcc gggctgacac agttgcccaa cgatgcgcgg ctttatcttg
    13381 cgcgcggagt attgcgggtg cagctaagcc agcttgatct tgccattgct gatttcgaaa
    13441 gcgcccaccg gctcgatccg aaattgtctc tcgccgagga tgctattggc atgatgcaga
    13501 gccagaagca ccagaatgcc gctgctctgg agacgttccg caaccaggcc atgcagcata
    13561 aggatgatgc cctgcttcaa tacctgtacg ccgaggccct gtcgcagcag gacaacaccg
    13621 gcccgcagca gacagccgag gccattgccg cgctcaggcg cgccgtggcc atcgagccgg
    13681 actaccgtcc tgcgcacgat ttgctcagta tgctgtacct gcgcgcaaat gatctgcctt
    13741 tggccatcag ccatgcagaa agagcactgc agttagaccc gaatgacgat acggctgtgt
    13801 atcaggaact gatggcgtac cgccgtcttg gtaaaaaaga gcaggtagac gctttggtag
    13861 cacgtctcaa ggaaatcaag caaactcagc agaatgcaaa gacgaagtac cttcttcagg
    13921 aagcccagcc ataaattcgc agaaagaggt gcatgatgta cagaccttcg agaagaaagt
    13981 tcatccagac gggcatagcc gcagcgggat cagccgcgat acggcctcgc gcatttgcca
    14041 tgttcgccgc aggcacagat gcgattgtcc cgcaactatc gcaatttaac tatggcgatg
    14101 tgcagttact cgacggccct atgctcgacc agttcaatgc aaaccatgcc ttctttctgg
    14161 caatagatga agacaagctg ctgaagccgt tccgcaaacg cgcgggtctc ccggcccccg
    14221 gtgaagacat gggaggctgg tacaacttct cagatgattt tgatccgcca cgcaatatga
    14281 ctggcttcgt tcccggccac agctttgggc agtatctctc ttcgttggcc cgcgcatacg
    14341 cggtgaccgg atcaaaaccg acgcaggaaa aagtacaccg tctcgtgcgt ggattcgctg
    14401 ctaccgtcag cgagaagttt tatgtggact acccgcttcc tgcctatact ttcgacaaga
    14461 tcaactgcgg cctgatggat gcacaccagt tcgctgccga tcccattgcg ctgtctgttt
    14521 tgaataaaga aacagatgcc gtccttccat tcctgccgga aaaagcgctg aaccgtcgcg
    14581 agcaggcgca gcgtccgcac aaaaacattg cctttacatg ggatgaaact tacaccctgc
    14641 cggaaaattt gtatctcgct tacaaacgcg gcgcaggcgg ccgctataag caactggctg
    14701 cccgcttcct cgaagacgac acttacttcg atccacttgc tgaaggacag aacgttttgc
    14761 caggagaaca cgcctacagc catgtaaatg cgttttcttc cgcaatgcag gcctatcttg
    14821 tcacaggaaa cgagaagtat ctgcgtgctg cgcgcaatgg ctttgaattt gtgcgcacta
    14881 cacagagctt cgccaccgga ggctgggggc cggatgaaac cttccgttcg ccagacagcg
    14941 gcgagatggg cgagagcctc accaggaccc atgccagctt tgaaacccca tgcggcgctt
    15001 atgggcattt caagattacg cgctacctgc tccgcgttac cggagacagc cgctatggcg
    15061 atagcatgga gagggtcctt tacaacacaa tcctcggcgc caggccgctt ctggaggatg
    15121 gtacgagctt ctattactcg gactataaca acaatgcttc aaagttctat catcaggaca
    15181 aatggccgtg ctgctccggc acattcccgc aaattacagc agactacgga attagttcct
    15241 acttccggca tcctcacgga atctatgtca atctgtatgt accgtcgaaa gtaagttgga
    15301 cacagaatgg tgcgcgctgc gagcttcgtc agcgcacgtc ctacccgctt gttccggaaa
    15361 cagagatgga agtcaatccg gaacgcccgg caaagttcgt agtagctgtg cgaattccgt
    15421 catgggcggg accaaaaaca tccgttgcaa tcaacggcaa gcgtatcgga cccgaaatca
    15481 ggcccggaac gttttttcct gtcgaacgcg aatggaaaaa aggcgaccga attgagattt
    15541 tcttcgatat gccaggcgtt ctggaagctg tggattcaaa gcatccaaat accgtggctt
    15601 tggtacatgg cccgcttgca ctcttcgcaa tcgagcccct gaatgtccag atgaaacgcg
    15661 cacagctact gtccgctgcg cagttgtccc gcggatcaga ccactggatt gtcgaaagcg
    15721 atgcaggacg catcactatg aagcccttcg cttccatctc aaatgagaag tacaggctct
    15781 accatgatgt aaatgcataa gctggagggt tgggtgtccc catcttcaag gacaccaaac
    15841 ctccagatca acaatatagg ccgaattact tcgccacagc ctgcgcagca tgaagttgtt
    15901 gcaacgcgtt caccgtggtt gtatgtttct gccgcgcctg aataccttcc gcggcagcgc
    15961 gcgctgccgc aagcgttgtg atcgtaggaa tgcgctgcat caccgccgcg cgccggattg
    16021 ccttctcgtc aaagaacggg tcttgaccat gcggcgtatt cacaatcaac tggatacgat
    16081 cgcccttgat caggtccacc acatttggac ggccttcctt caccttgtaa acgcgctcga
    16141 ccaccatccc ggcctgttca agcacttggg ctgtgccttc ggtagcaacc aggtggaagc
    16201 ccaactcgac atattgccgc gccacatcca ccacctgcgc cttgtcgcgg tcattcacgc
    16261 tgaagaagac cgtgcctttc agcggcagat gctgtccggc agaaagttgt gcctttgcaa
    16321 atgcttcacc gaagttgtcc gccacgccca tcacttcgcc cgttgacttc atctccggac
    16381 caagtacggt atctactccc ggaaatttgt tccacggaaa gaccggagac ttcacaaagt
    16441 agtgtgttcc ggtttcgagg tcccttccgg aagctacata ctcagacaga aagtcgcgca
    16501 gcctgcggcc cgtcatcaga cgcgaagcaa tcttcgccaa cggcacgccg gtggccttcg
    16561 acacatacgg cacagtgcgc gaggcgcgcg gattgacttc gatcacgtag accttgtcgc
    16621 gctggatcgc aaattgcagg ttcaccagac cgacaacatt cagtgccagc gccagtttgc
    16681 gtgtataggt gcggatggtg ttgagcacct ccgctgagag atctaccggc ggcagcacac
    16741 acgaagagtc gccggagtgg atacccgcct cttcgatgtg ctgcatgatt ccggcgatga
    16801 cgacatccgt gccgtcgcag agcgcgtcta cgtccacctc gactgcatct tccaggaagt
    16861 ggtcaatcaa cacaggccgc tcctgcgagt actcaacggc ctccttcatg tagcgcgcga
    16921 ccgcatcgct gtcaaaagcg atggtcatgg cgcgcccgcc gagcacatac gatggccgca
    16981 ccagcaccgg ataaccaatc cgctccgcgc ctgcaagcgc ctcttccacg ctggtggcca
    17041 tagcgcccgg cggctgcgga atgttcaatt cttcaagcag tttgccaaag cgcttgcggt
    17101 cttcggccag gtcaatggac tccggagaag tcccgaggat cggcacaccg gcctctttca
    17161 gccgcagcgc caggttcagc ggggtctgcc cgccaaactg cacaatcatg ccaatctctg
    17221 cgccgctctt cgcctcgtgc tcatacacag cgagcacatc ttcgagcgtc agcggttcaa
    17281 agtagagacg gtcgctggta tcgtagtcgg tcgaaaccgt ctcaggattg cagttgacca
    17341 tgatggtctc gtagccgtct tccttcagcg caaacgcagc atggcagcag cagtaatcaa
    17401 attcaattcc ctgcccgatg cggttcggcc cggcgcccag aatgatgacc ttgtgcttgc
    17461 tggtcgggat ggcctcatct tcctcgtcgt agcaggagta gagatacggc gtcatgctct
    17521 cgaattcagc cgcgcatgtg tccaccagtt tgtagacagg ctgaatgcca tgccggtttc
    17581 gcagttgccg cacctgcttc acaccttcgc tgccggaaag cccccagacc tcggcgatac
    17641 gctcatcgga aatccccatg cgcttggctt tgcgcaatgt ttctgctgag accgtgtccg
    17701 gattgtagtc ggccagcgat gcgatcatgt ccgtgacttc cttgatgtgg tacaggaacc
    17761 acggatcaat cgaagtcatg cgcgccactt cgcgcacgct cagaccgcgg cggaaggcaa
    17821 agcgaatata gtcaatgcgc ccgggcttgg gcgtaaccag catctgtgtc agacgccgcg
    17881 ggtcgagtat ctccgcgccg actttctttc ctgtatccag cgagcgcagc gccttcatca
    17941 gcgattcctt gaaggtgcgg ccaatcgcca tgacctcacc caccgatttc atctgcggcc
    18001 cgagattttc atctgctccc gggaacttct caaactgcca cttcgggatc ttcgtaacga
    18061 cataatcaat cgtcggttca aagcacgccg gggtcatctt cgtaatgtca ttcggaatct
    18121 catccagcgt gtagcccacg gccagctttg ccgcaatctt cgcaatcggg aagcccgtcg
    18181 ccttcgaggc cagcgccgag gagcgcgata cacgcgggtt catctcgatg accgtcatgc
    18241 ggcccgtcag cggattcacc gcaaactgca cattcgaccc gcccgtctct acgccgattt
    18301 cgcgcatcac cgcaatcgca gccgtgcgca tcttctggta ttcacgatcg gtaagtgtct
    18361 gcgctggagc aatcgtgatg gaatcgccgg tatggacgcc catcgggtcc atgttctcaa
    18421 tcgagcagac gataatgacg ttgtccgcca ggtcccgcat cacctccagc tcgtactctt
    18481 tccagccgag aaccgattct tcaatcaggc actcatgcac cggcgagagg tccagtccgc
    18541 gggcgagaat ttccattagc tcttcgcggt tgtaagcgat gccgccgcct gatccgccta
    18601 gagtgaacga aggccggatg atcgccggga aaccgatctt ggtaacaaac tccagcccat
    18661 cgcggatatt gttcaccagc gccgatttcg gcacgtcgag accgatgcgc gtcatcgcgt
    18721 ctttaaacaa gaggcggtct tctgccttct tgatggcatc cagcttggcg ccaatcagct
    18781 caacgttata tttttcgagt acacccgaat cggccagttc tactgcaagg ttcaacgccg
    18841 tctggccacc gacagtgggc agcagagcaa actggccttt gcggccttcg tccttgagca
    18901 tctgcgattc aaggcgaatg atctcttcca catactcgcg cgtcagtggc tcaacatagg
    18961 tacggtcagc cagctcagga tcggtcatga tggtggccgg gttggaattc accagcacca
    19021 cttcataacc ttcctgcttg agcgccttgc acgcctgcgt acctgagtag tcaaactcag
    19081 ccgactgccc aatcacaatc ggccccgagc cgatcacgag aatcttggtg atgtcattcc
    19141 tgcgtggcat ctat
//
LOCUS       DASRBV010000391         6728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_189520, whole genome shotgun sequence.
ACCESSION   DASRBV010000391 DASRBV010000000
VERSION     DASRBV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189520"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFA02_14215"
     CDS             complement(<1..791)
                     /locus_tag="VFA02_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU11056.1"
                     /translation="MAIYLPGTAEDQELALEELTPREIVAELDKYVVGQKAAKRAVAI
                     ALRNRMRRQKLPPDLAEDIMPKNIIMIGPTGVGKTEIARRLAKLTNSPFLKVEASKFT
                     EVGYVGRDVESMVRDLVEIAIDMVREEKLEEVEDKAELNAEERLLDLLLPPPAPAPAG
                     QSTNQSSSTAISTDSHQRTREKLRQQFREGKLDERMVEIDVRERNAPSFEIISSQGIE
                     EMDINIKDMLPNIFGQRTKKRKMKVAEAFEYLVQEEEGRLVDMDQV"
     gene            complement(824..1405)
                     /gene="hslV"
                     /locus_tag="VFA02_14220"
     CDS             complement(824..1405)
                     /gene="hslV"
                     /locus_tag="VFA02_14220"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZU11057.1"
                     /translation="MSKQRPSLYSVSGSSPRIRSTTVVCVRRNGSVVMAADGQVTLGE
                     SVIKHSARKIRRLYQDKILAGFAGSTADAFSLFSRFESKLEQYAGNVGRAAVELAKDW
                     RTDKILRNLQALLIVSDPHQTFLISGSGDVIEPDEGIAAIGSGGSYALAAARALYQNT
                     ELSAREIAEKSLRIAGQICIYTNDQITIEELHT"
     gene            1669..3276
                     /locus_tag="VFA02_14225"
     CDS             1669..3276
                     /locus_tag="VFA02_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HZU11058.1"
                     /translation="MKMMRSFACFFSLLALFGWMALPFRAVAQNDGASAPKLIEKDGR
                     YALLVDGRPYLILGGQINNSSAWPATLPEVWPLIEKMHANTVEAPVYWEQMEPRPGTF
                     DFTNVDALITQARQHNVHLVLLWFGTWKNGKMHYVPEWVKTDTQHYPRMVNERGEPID
                     VLSPNSAANLEADRRAFTALMRHVRETDGSQHTVLLVQVENESGSLGAVRDFSPAAQK
                     QFEGTVPAELVKALGKHAGTWKEVFGPDADETFAAYSVAHYIDQVAQAGKAEYPLPMY
                     CNVWLGSRQQIPGVNYPSGGAVPHMLDVWKATVHSIDMIGPDIYIDDSAMYRQVLDAY
                     GRRDNAMWVPETGGGDDYARYFYYALAHGAIGFSPFGMDQTGWTYGRDEYPKLHSDNY
                     ALLGPMDREIAEWNFEGKLKTAVEEPGQPRTTLNFGKWKATVSFGLPQWGEARAMGNK
                     EHDGRALIVQIAEDEFLVTGFGARVQFQLVDQNTREHMQFLRVEEGRYENGTWKTSRL
                     WNGDQTDYGLNFSRQGAKVVRAYLGTY"
     gene            complement(3342..4115)
                     /locus_tag="VFA02_14230"
     CDS             complement(3342..4115)
                     /locus_tag="VFA02_14230"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZU11059.1"
                     /translation="MSVMVSPMRIQEFARRTGTTVRALHHYDRLGLLRPSRTSAGYRL
                     YGERELVRLQQITTLKFIGCSLKEIKAVLNGPAPSLDEILALQHDALERKRKAVDRAL
                     SAVERAQKLFIRTGKADWEALKQIIEVIQMEQDKEWMKKYFTTEQLNAIKARGDADPE
                     AVRKGQEAWAKLIPDVEAAAQKGLDPASEGARSLAQQWVELLAGFTGGDPEIQQGLNR
                     LYADKQNWPQSFKRPYSEEAGQFIRAAMKAHGLSCLSPL"
     gene            4265..4642
                     /locus_tag="VFA02_14235"
     CDS             4265..4642
                     /locus_tag="VFA02_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11060.1"
                     /translation="MRRLLAISMLVLLGLPLLAPVFAPDAAEASIPACCRRGGRHHCA
                     MSEMAAQPSSGHAISVMAEKCPYAPAVAPVLHFSAFAPPSDAAIFAQIVSHPAVYAQT
                     EARYRISFDRARQKRGPPVTGLL"
     gene            4821..>6728
                     /locus_tag="VFA02_14240"
     CDS             4821..>6728
                     /locus_tag="VFA02_14240"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU11061.1"
                     /translation="MRRVLLLFVLACMGLGQHARATVFGQVEGIVHDPQHRPIAGAQV
                     KLRSTTSDFSLTAQSNQDGEFHFAAVPVGNYTVSVQQQGFSPVKKNIVVVSNASPVLH
                     FELSIAGAHETTVVHEAEQSANVDTATTTTLVTREDIARAPGAGRTNGFQMITNYVPG
                     SYETHDMLHIRGGHQVSWLIDGVQIPNTNIGSNLGAQIDPKDIDYLEVQRGSYAADVG
                     DRTYAVFNVVPRTGFEGNNQGELVLSAGNFYQTNDQLNLGSHSEKFAYYLSLNGNRSN
                     LGLQPPIGKAYHDAVNGYGGFGSFIYNRTPQDQFRLVTQLRADYFQVPYDPDPNSWEN
                     QQYNSSGLRDGQHETDGLVAFTWAHTYNAQSLLQISPFYHYNSANYEPKPYDFPVATA
                     GRRASNYAGLQGSFGTEIARNTLQAGIYTFGQHDANTFGTIFNDGSYQNFSVHDAASG
                     GLIEEYISDNYKITPWLALIGGLRQSWFRGGFTEKATSPRVGAALQVPKLGWVFRGFY
                     GHYYQPPPLLTASGPVLGYANATNTSFLPLHGERDEEHQFGVAIPFRGWMLDADTFRT
                     RAKNFLDHSTLGESSLYYPVTIDGALIRGWELTLRSPRVWRYGQAHLAYSNQIAEQRG
                     ALTGGLVCNPET"
BASE COUNT         1358 a         1870 c         2003 g         1497 t
ORIGIN      
        1 acctggtcca tgtccaccag ccggccctct tcttcctgca ccagatattc gaaggcctcg
       61 gccaccttca tcttgcgctt ctttgtgcgc tggccaaaga tgttcggcag catgtctttg
      121 atattgatgt ccatctcctc aatgccctgg ctggagatga tctcaaatga aggagcattg
      181 cgctcccgca catcaatttc aaccatgcgc tcatcgagct tgccttcccg gaactgctgg
      241 cgcagctttt cgcgcgtgcg ctggtgcgag tcagtggaga tggccgtgct gctgctctgg
      301 tttgtgctct gcccggcagg cgccggcgcg ggtggaggaa gcagcaggtc cagcagacgc
      361 tcctcggcgt tcagttcggc cttgtcttct acttcttcga gcttctcttc gcgcaccatg
      421 tcaatcgcga tctcgaccag gtcgcgcacc atggattcga catcgcggcc cacgtaaccg
      481 acttcagtaa atttcgaggc ctcgaccttc agaaagggcg agttggtcag cttcgccagc
      541 cgccgtgcaa tctcggtttt gcccacgccc gttggcccaa tcatgatgat gttcttgggc
      601 ataatgtctt cggcgaggtc gggcggaagc ttctggcggc gcatgcggtt gcgcagcgct
      661 atggcgacgg cgcgcttggc ggccttctgg ccgacgacgt atttgtccag ctcggcgaca
      721 atctcgcgcg gagtcagttc ttcaagcgcg agttcctggt cttccgctgt cccgggtaaa
      781 taaatggcca tgtgcccgct cctttctgag aggcgcgtaa caattaagtg tgtaattctt
      841 cgatggtgat ctggtcattg gtgtagatac agatctgtcc ggcaatgcgc agcgattttt
      901 cagcgatctc gcgcgctgac agctctgtat tctgatatag cgcgcgcgct gctgccagag
      961 catacgatcc gccgctccca atggcagcaa taccttcgtc cggctcaatc acatcgccag
     1021 aaccgctgat cagaaacgtc tggtgcggat cagagacgat aaggagtgcc tgcaggttac
     1081 gcagaatctt gtctgtgcgc cagtcttttg caagctcgac ggctgcgcgc cctacgtttc
     1141 cggcatactg ctccagcttg gactcgaagc ggctgaacag agaaaaggcg tcggccgtag
     1201 atccggcaaa tccggcaagg atcttgtcct gatacaggcg gcggatcttc cgggccgaat
     1261 gcttgatgac cgattcgccc agagtcacct gtccgtcagc agccatcaca acgctgccat
     1321 tgcgccggac acagacgaca gtagttgaac ggatgcgcgg agacgacccc gaaacggagt
     1381 aaagggaagg tctttgcttc gacatgctta aagcccagta tatcgtagcg ctccagaaga
     1441 tggcaccgag ggaagtcagt gaaagcttat ggtcttttcg tatatggatc atccgtcatt
     1501 ccggcggcag aaggcaggaa gaatcttagc gatgtgaggg tcaagatagc gtcacgattg
     1561 aaatcgtgca ttccggcgaa actctctcca ggctggaaac agggcctgag acattcaggt
     1621 ggcttttcaa tagcacagct tgcctttgac tgatgtagtc tgttgcgcat gaagatgatg
     1681 cgctcgtttg cctgcttctt ttcacttctt gcactgttcg gctggatggc tctccccttt
     1741 cgcgccgtcg cgcaaaacga cggggcatcc gcgccgaagc tcatcgaaaa agatgggcgg
     1801 tatgcgttgc tggtagatgg caggccgtat ctgattcttg gcgggcagat caataactcc
     1861 agcgcgtggc ctgcaacgct gcccgaagtc tggccgctga ttgagaagat gcacgccaac
     1921 accgttgagg caccggttta ctgggagcag atggagccca ggccaggcac gttcgacttc
     1981 accaatgtgg acgcgctcat cacacaggcg cggcagcaca acgtgcatct ggtgctgctg
     2041 tggtttggca catggaagaa cggtaagatg cactatgttc cggaatgggt gaagacggac
     2101 acgcagcact atccgcgcat ggtcaatgaa cgcggcgagc cgattgatgt tctttcgccg
     2161 aactccgcag cgaaccttga ggccgacagg cgcgccttca ccgcgttgat gcgccatgtg
     2221 cgcgaaaccg atggaagcca gcacacggtc ctgctggtac aggtggagaa tgagtccggc
     2281 tcgcttggag cggtgcgcga tttttcgcct gcggcgcaga agcagtttga aggaactgta
     2341 ccagccgaac tggtgaaggc cctcggaaaa cacgcaggca cgtggaaaga ggtctttggg
     2401 ccggatgcgg acgagacctt tgcagcgtac tccgtagcac attacattga tcaggtggcg
     2461 caggcaggca aagcggaata tccgctgcct atgtattgca atgtgtggct ggggtcgcgg
     2521 cagcagattc cgggagtgaa ttatccgagc ggcggtgctg tgccgcatat gctcgacgta
     2581 tggaaggcaa cagttcactc catagatatg attgggccgg acatctacat tgacgactct
     2641 gccatgtacc ggcaggtgct cgatgcttac ggccgcaggg acaatgcgat gtgggttccg
     2701 gaaaccggcg gcggcgatga ctacgcgcgc tacttctatt atgcgctggc gcatggcgca
     2761 atcggctttt ctccattcgg aatggaccag acgggctgga catatggccg ggatgagtat
     2821 ccaaaactgc acagcgataa ttacgcgctg ctggggccca tggaccgcga aattgccgag
     2881 tggaactttg agggcaagct gaagacggcg gtggaagaac ccgggcagcc gcgcacgacc
     2941 ctcaatttcg gcaagtggaa ggcgaccgtg agcttcgggc ttccgcaatg gggcgaggcg
     3001 cgggcgatgg gcaacaagga gcacgatggg cgcgcgctga tcgtgcagat tgccgaagat
     3061 gaatttctgg taacgggctt tggcgcgcgg gtacagttcc aacttgtgga ccagaacacg
     3121 cgcgagcaca tgcagtttct gcgcgtggaa gaaggccgct atgaaaatgg cacgtggaag
     3181 acatcgcgcc tgtggaacgg cgaccagacg gactatgggt tgaacttcag ccgtcagggg
     3241 gcaaaggtgg tgcgggcgta tttgggaacg tattgaactc cggcgctgga agggtaagca
     3301 catgcggcct tctgtcaaga cagaaggccg gcatgtttac ctcacagggg cgaaaggcaa
     3361 ctcagtccat gtgctttcat agcagcacga atgaactgac cggcctcctc gctgtaagga
     3421 cgcttgaacg attgtggcca gttctgcttg tcggcataaa ggcggttcag gccctgctga
     3481 atttcagggt cgcctcctgt aaagcctgcc agcagttcca cccattgttg cgccagggac
     3541 ctggcgcctt cgcttgcagg atcaaggccc ttctgggctg ccgcttccac atcaggaatc
     3601 agctttgccc atgcctcctg tcctttccgc accgcctcag ggtccgcatc gccgcgggcc
     3661 ttgatggcgt tcaactgttc cgttgtaaag tactttttca tccattcctt gtcctgttcc
     3721 atctggatca cctcaatgat ttgtttcaag gcttcccaat cggcctttcc cgtccggatg
     3781 aagagcttct gcgcacgctc gacagcagag agggcgcgat ctactgcttt tcgttttctc
     3841 tcaagcgcgt cgtgctgcag agcaagaatt tcgtccagac tgggagcagg accgttcagg
     3901 accgctttga tttccttcag agagcagcca atgaacttca gcgttgtgat ctgttgcaac
     3961 cgcaccagtt cgcgttcgcc gtacaagcga tacccagcgg acgtccgcga aggccgcagc
     4021 agccccaggc ggtcatagtg gtgcaacgcg cgcaccgtag ttccggtacg gcgtgcaaac
     4081 tcctggatcc gcatgggaga aaccataacg ctcattttct gcctccagga acgatggtca
     4141 gggatgacgc aacgtcaggg tcaagtagtt atttttatat tttctaccgg gcctggttta
     4201 cagtgtgccg ccgcgttcat ctggtccaga gacttccacg caggattttc agatacactg
     4261 aagcgtgcgg cgactgcttg ccatttcgat gctggtgctt ctggggctgc ctctgcttgc
     4321 gcctgtgttc gcgccggatg cagccgaagc gagcattcct gcatgttgcc gaagaggcgg
     4381 caggcaccac tgcgccatgt cggaaatggc tgcgcagcca tcgtcgggtc acgccatcag
     4441 cgtgatggct gaaaaatgcc cgtatgctcc ggcggtcgct ccggtgcttc atttcagcgc
     4501 atttgcgcca ccctctgatg cggccatctt tgcgcaaatt gtcagccatc ctgctgttta
     4561 tgcgcagacg gaagcacggt atcgcatttc gtttgacagg gcgcggcaga agcgcggacc
     4621 tcctgtaact ggtctcctct agccgtgcgc tgcatcaggg agattgtttc cagttgaggt
     4681 ccttcccctt tctcttcact atgttcagag tcagggccag aatggcaatt catttgtgat
     4741 gcgttcttcc caggcagcgc acgcactcct cttttcaatt acagctctta ccttagctat
     4801 ccgaggaggc atctgccgaa atgcgcagag ttctattgct gtttgttctt gcgtgcatgg
     4861 gtctgggcca gcacgcgcgc gccactgtct ttggacaggt ggagggcatt gttcatgacc
     4921 cgcagcaccg gcccattgcc ggcgcgcagg tcaagctcag gtccacaaca tccgattttt
     4981 cgcttactgc acagtccaat caggacggag agtttcattt tgctgctgtt ccggtgggga
     5041 attacacggt gagtgtccag cagcaggggt tcagcccggt caaaaagaac atcgtggtgg
     5101 tctccaatgc gtcacctgtg ctgcattttg aactgagcat tgccggcgcg catgagacca
     5161 cggtggtcca cgaggcggaa cagagcgcga atgtggatac ggccacgacg accacgctgg
     5221 tgacgcgcga agacattgcg cgtgctccgg gggcgggccg gaccaacggc ttccagatga
     5281 ttacgaatta cgtccccggc tcctacgaga cgcacgacat gctgcatatt cgcggcggcc
     5341 atcaggtgag ctggctgata gacggcgtgc agattcccaa caccaacatc ggcagcaatc
     5401 tcggcgcgca gattgacccg aaggacattg attatcttga agtgcagcgc ggaagctatg
     5461 cggccgacgt gggcgatcgt acctatgcgg tcttcaatgt ggtgccgcgt actggatttg
     5521 agggaaacaa tcagggtgag ctggtgctga gcgcgggaaa cttctaccag acgaatgatc
     5581 agcttaatct gggcagccac tcggagaagt ttgcttacta tctcagcctg aatggaaacc
     5641 gcagcaatct gggcctgcaa ccccccattg gcaaggccta ccacgatgcg gtgaatgggt
     5701 atggcggctt cgggtcattt atctataaca ggacacccca ggaccagttc cgtctggtga
     5761 cacagttgcg cgcggactat ttccaggtgc cgtatgatcc tgatccgaat agctgggaaa
     5821 accagcagta caactcgagc ggtttgcgcg acgggcagca tgagaccgac gggctggttg
     5881 cattcacctg ggcgcatacg tataacgctc agtcactgtt acaaatttcg cccttctacc
     5941 actacaacag cgcgaactac gagccgaagc cgtacgactt tcccgtggct acggcgggcc
     6001 gtcgggcctc aaactacgca ggcctgcagg gctcttttgg cacggagatt gcgcggaaca
     6061 cgttacaggc tgggatttac accttcggtc agcatgatgc caacacgttc gggaccatct
     6121 tcaatgacgg gagctatcag aatttctcgg tgcatgatgc ggcttcgggt ggcttaatcg
     6181 aggaatatat ctcagacaat tacaagatca ccccgtggct tgcgctgatt ggcggcctgc
     6241 ggcagtcgtg gttccgcggc ggttttactg agaaggcgac gagtccgcga gtgggggcgg
     6301 cgttgcaggt gccaaagctg ggctgggtct ttcgcggatt ttacgggcat tactatcagc
     6361 ctccgccgct gctgacggcc tctggcccgg tgctcggcta tgcgaatgcg acgaacactt
     6421 cctttctgcc gctgcatggc gagcgcgatg aggagcacca gttcggcgtc gcgattccgt
     6481 ttcgcggatg gatgctggat gcggacacct tccgcacccg ggcaaagaac ttcctcgacc
     6541 acagcactct cggtgagtcg agcttgtact atccggtgac gatagatgga gcgctgattc
     6601 gcggatggga gctgacgctg cgctcgccgc gtgtatggcg gtatgggcag gcgcatcttg
     6661 cctattcgaa ccagattgcc gaacagcgcg gggccttgac cggcggcctg gtctgcaatc
     6721 ctgagaca
//
LOCUS       DASRBV010000392         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_502546, whole genome shotgun sequence.
ACCESSION   DASRBV010000392 DASRBV010000000
VERSION     DASRBV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502546"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VFA02_14245"
     CDS             complement(<1..1727)
                     /locus_tag="VFA02_14245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZU11062.1"
                     /translation="MHRWSRLFVPTLREAPADAEVPSHKFLVRAGYIRQLGAGIYSYL
                     FLGQRSIQKIIGIVREEMDKIGQEFLLPTLHPAELWQESGRWTGMGQNMFRLQDRKGA
                     WLCLGMTHEEVMTSIARNELRSYKQLPQIWYQIQTKFRDEPRPKSGLLRVRQFIMKDS
                     YSFDLDAAGLDKSYDLHDQVYRTIFTRCGLNFVAVEADSGAMGGSQSQEFMVYTDAGE
                     DFIASCAKCHYAANLEKAASRLAPVTELAPTGDGKPELVHTPGIAAVADVAKFFGISE
                     AQDIKCVAYMAEKKAAKGESSWHPVAVFLRGDHQVNETKLLGLIKATDLRPMQADELD
                     QYIHGPAGYLGPIGVTPAKSLDEDELVIVLDRALEGRQNMVCGANKLDYHYRNVTPGR
                     DFSWTTVADVRNVVEGEGCPQCGNPLRVAKAVEVGHIFKLGYKYSESMGARVLDQNGK
                     EVTPIMGSYGIGIERILTACIEQNHDANGFWLPRSIAPFDVVVTITNAADANLVSIGE
                     RIAADLEAAGFDVLLDDRDERAGVKFKDSDLIGVPYRVNVGKKAAEGKAELVTRATAS
                     SEDVQISELV"
     gene            complement(1801..2943)
                     /locus_tag="VFA02_14250"
     CDS             complement(1801..2943)
                     /locus_tag="VFA02_14250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU11063.1"
                     /translation="MQLLHVIPSTDPATGGPAQGLRCILKGHERLGCSAEVLSNDAPA
                     SQHDTDIQSPVHHLGPGKGVYGYNTRLLFWLRENYARFDGVIAHGIWQYPALAVRRTV
                     LGKRPYVVFTHGMLDPWFRKAYPLKHLKKYLYWLLALYPVLRDAKRVLFTSPLEQELA
                     PKSFSPYKCHGEVVPYGTLPPEDDPELSKKAFHRLCPSVKGHRYLLFIARLHEKKGCE
                     LLLQAFANVCKDQPDFDLVMAGPDNVGLKTKLQSLAMELGIAERVHWPGMLNGDAKWG
                     AFRNCEAFILPSHQENFGIAVAEAMACARPVLITNKVNIWPDIVQSHSGLVEDDTLEG
                     TGSLLQRFLRLDEAERTSMGENGMAFFHAHYNSLETAAAIQKIFSI"
     gene            complement(2971..>3312)
                     /locus_tag="VFA02_14255"
     CDS             complement(2971..>3312)
                     /locus_tag="VFA02_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZU11064.1"
                     /translation="HKNLSEADLRERERRPVPFHVIINPKIELLTEPDVAYHEGCLSL
                     PGFMAVVPRARKVRVTCLDERGTQKIIEASGWYARILQHEIDHLNGRLYIDRMKSETF
                     STLENYQRCHR"
BASE COUNT          714 a          886 c          941 g          771 t
ORIGIN      
        1 acgagttcgc tgatctgcac atcttcgctg gatgcagtgg cgcgggtcac cagctctgct
       61 ttgccttcag cggccttttt gcccacattg acgcgataag gcacgccgat gaggtcggaa
      121 tctttgaatt tgaccccagc gcgttcgtcg cggtcatcga ggagaacgtc gaatccggct
      181 gcctcaagat cagcggcaat gcgctcgccg atgctgacaa ggtttgcgtc ggcggcgttg
      241 gtgatggtca cgacgacatc aaatggggca atggagcgcg gcagccagaa tccgtttgcg
      301 tcgtggttct gctcgatgca ggcggtaagg atgcgctcga taccaatgcc atagctgccc
      361 atgatgggcg tcacttcctt gccgttctgg tcgagcacgc gggcgcccat gctctctgaa
      421 tacttgtagc caagcttgaa gatgtgcccc acttcgactg ccttggcgac cctcagcgga
      481 ttaccgcact gcggacagcc ttcgccttcg accacgttgc gcacatcggc gacggtggtc
      541 caggagaagt cgcggcccgg agttacgttg cggtagtgat agtcgagctt gttggccccg
      601 cagaccatgt tctggcggcc ttcaagtgcg cggtcgagga cgatgacgag ttcgtcttca
      661 tccagcgact ttgccggagt tactccaatc gggccgagat atcctgccgg tccgtgaatg
      721 tactggtcga gttcgtcggc ctgcatcggg cgcagatcgg ttgccttgat aagcccgagc
      781 agtttcgttt cattcacctg atggtcgccg cgcagaaaga ccgctaccgg atgccaggaa
      841 gactcgcctt tggcggcctt tttctcagcc atataggcca cgcacttgat gtcctgcgct
      901 tcagagatac caaagaactt tgctacatct gcgactgcgg caatgccggg agtatgtaca
      961 agctccggct tgccgtcgcc tgtgggagca agttccgtga ccggagccag gcgcgaggcc
     1021 gccttctcaa ggtttgcggc gtaatggcat ttggcgcagc ttgcgatgaa gtcctcgcct
     1081 gcgtcggtgt agaccatgaa ctcctgcgac tgcgagccgc ccatcgcgcc ggagtcggcc
     1141 tcgacggcga caaagttcag gccgcagcgc gtgaagatgg tgcgataaac ctgatcgtgc
     1201 aggtcgtagc tcttgtccag tccggcggcg tcgagatcaa aggaatagct gtccttcatg
     1261 atgaactggc gcacgcgcaa caggccggac ttcgggcgcg gctcatcgcg gaacttggtc
     1321 tgaatctgat accagatctg cgggagctgc ttgtagctgc gcagttcgtt gcgcgcgatg
     1381 gaggtcatga cctcttcgtg cgtcatgccc agacaaagcc atgcgccttt gcggtcctgc
     1441 agacggaaca tgttctgccc catgccggtc cagcggccgc tctcctgcca aagttcggcg
     1501 ggatgcagcg tcggcagcaa aaattcctgt ccgattttgt ccatctcttc gcgcacgatg
     1561 ccaatgatct tttggatgga gcgctgccca agaaacagat aggagtaaat gccggcgccg
     1621 agctggcgga tgtatccggc gcggacgagg aacttgtggc tcggtacctc ggcatctgcc
     1681 ggcgcttcgc gcagtgtggg aacaaaaagc ctggaccaac ggtgcataat gtgcgctttc
     1741 cgaagatgaa taggtaaaac tctattttag ctggcttttg aatttacagc ccattgcagg
     1801 ctatatggag aagattttct gaatcgccgc cgccgtttcc agtgaattgt aatgagcatg
     1861 gaagaatgcc atcccgtttt cacccatgct ggtgcgttca gcttcatcca gtcgcagaaa
     1921 gcgctgcaaa agagaccctg ttccttccag cgtgtcatct tccacgaggc cggaatggct
     1981 ttgaacgatg tccggccaga tattgacttt gttggtgata agaacagggc gtgcgcaggc
     2041 cattgcttca gcaacagcta ttccgaaatt ttcctgatgc gagggaagga tgaatgcctc
     2101 gcaattgcga aaggcacccc atttggcatc accgttcagc attcctggcc aatggacacg
     2161 ctcagcaatt ccaagttcca ttgccagaga ttggagtttg gtttttaacc caacattgtc
     2221 agggcctgcc atcacaaggt caaagtctgg ctgatctttg cagacattgg cgaatgcctg
     2281 tagcagcaat tcacagccct tcttttcgtg cagacgggcg atgaagagca gatagcgatg
     2341 tccttttacc gaagggcaaa gcctatggaa tgccttcttt gaaagctccg gatcatcttc
     2401 aggtggaagc gtgccgtaag ggaccacctc gccatggcac ttgtatgggc tgaaggattt
     2461 tggcgcaagc tcctgctcaa gtggagatgt aaataacacg cgctttgcgt cacgcagcac
     2521 tggatatagg gcgagaagcc agtaaagata ttttttcaga tgcttcaacg gatatgcttt
     2581 ccggaaccat ggatcaagca tgccatgcgt aaagacgacg taaggacgct tgcccagaac
     2641 tgttcgtcgc accgccaaag ccggatattg ccatattcca tgtgcgatga ctccatcaaa
     2701 acgagcataa ttttcgcgca gccagaaaag gagacgagtg ttgtaaccat aaacaccctt
     2761 tcccggccca agatgatgca ccggcgattg aatatcagta tcatgctggc ttgcgggagc
     2821 atcgttcgag aggacttcag cgctgcaacc taatcgctca tgacctttta aaatgcacct
     2881 gagtccctgg gcagggccgc cggtcgcagg atcggtgctt ggaattacat gaagtaattg
     2941 catatggagg aagatgatat gttgaatgct tcatctatgg catcgttgat aattctccag
     3001 agttgaaaag gtctcacttt tcatgcgatc aatgtaaagc cggccgttga gatggtcaat
     3061 ttcatgttgc aggatgcgcg cataccagcc gctggcttcg ataattttct gcgtgccacg
     3121 ttcgtccaga caggtgacgc gtactttgcg ggcgcgcggc acaacagcca taaatccggg
     3181 caggctgagg cagccttcgt gataagccac atccggctcc gtgagcaatt caatctttgg
     3241 attgatgatg acgtgaaaag ggacaggcct gcgctcacgc tcgcgcagat cggcttcgct
     3301 cagatttttg tg
//
LOCUS       DASRBV010000393         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_647477, whole genome shotgun sequence.
ACCESSION   DASRBV010000393 DASRBV010000000
VERSION     DASRBV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647477"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFA02_14260"
     CDS             <1..480
                     /locus_tag="VFA02_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11065.1"
                     /translation="REVGDHLGQIYEKQGRKQEAANIYALAAASGRITPDPAGRTTME
                     DRMDRLKEQGIHASYPDPGRELGEQRSVYLPKLTKKYASAEFFIALSPDKVEDVQFIR
                     GDEALKSAGDALRKSNFHMPFPKDSKAKLVRRGILACTPAMEKCQFTLLLPQTTTTQ"
     gene            578..1990
                     /locus_tag="VFA02_14265"
     CDS             578..1990
                     /locus_tag="VFA02_14265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZU11066.1"
                     /translation="MISQTITSGAGQNYSWVYDRYGNRWQENPNGAQMVFNKANNQIT
                     TSGYSYDAAGNLISDGVNPYSYDAEGNLVQEITGSGTVKFSYNALNQQVRTDWLSSGS
                     AAEVAFNLSGQLASLWWASDGSLLLGKAYLGSIPLESYLPRTNMAYFQHRDWENTERV
                     ITDASGNVTNIRISQPFGDGAANTTGSRDNTYDGFTGLWDGAASSTNHAQYREYSNIE
                     GRWLSPDPYDGSYDLTNPQSFNRYSYVGNNPLGFVDPSGLDGKSSTITTGGCTGAIMS
                     GGAAWGADLACLFGLLHLFGGPSFHGTLRPRPEGTGNPDWANVDGSLGETLGLPSDIN
                     WSKINPGIAGALGLPDAGCEFGSCGDIGSIASSFTQGAAIAAPLTWCGEHPTICTAGA
                     DLAAFVRAIPAVTASVLLLRMEGDGPATAPGVDRAACYEQYERDLDVCRKNHKRSCYA
                     QAMERYSACLAGKSAPPFNF"
     gene            2002..2319
                     /locus_tag="VFA02_14270"
     CDS             2002..2319
                     /locus_tag="VFA02_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11067.1"
                     /translation="MEHTLEVIAERNLTLDSDGIERVVTVQIGRPYVEPDGAWFCPFN
                     VLGLAPAGTRRAGGVDAIQALQLALVMIGAELSASSKSLKWDGEPSTGFPASIHDPVL
                     GPE"
     gene            complement(3615..5402)
                     /locus_tag="VFA02_14275"
     CDS             complement(3615..5402)
                     /locus_tag="VFA02_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZU11068.1"
                     /translation="MLQLKTINDILFLAASNANPRAVLYMDADGNWQPFTGTQIYQRV
                     RTLAEALGGWGIRKGDRIALLAENRWEWAVTDFAALAMGAVDVPIYPTLTAEQIGGLL
                     ADSGSRVAVVSSKQQYEKVAAVREKTALEHIILMDDEGVPDAVLMSSLLKGADARGPE
                     RDAAFDRAAYDVGPDDLATLIYTSGTTGEPKGVMLTHGNIAQNVNYSLAGFEISQQDS
                     CISFLPLSHITARHLDYAIYTRQATVAYCPSFEKLPGALQSIRPTVLVAVPRVFEKVR
                     QEAERQAAQSGWKKKIFHWAVKIGQSHREEILRGEAPSSLGWKLANKLVFSKIQHGFG
                     GRVRYYIAGGAPLGMDTAGWFAGVGIRILEGYGLTETSPVIAINTTAAYRIGSVGKPL
                     PNVECRIAEDGELLVRGPNVFSGYWHKPDETAEAFDAEGWFRTGDIGHIDGDGFLYIT
                     DRKKELLKTSGGKMVAPQPIENKLKANLLVGQAAVVGDKHKFIAALISPNFQALEEWT
                     RQQGITAPTRRELVEHPAVVARYQEIVNEVNSSLAHFETMKRFRIVPEEWSLASGELT
                     PSLKLKRRVIYEKYAKEIAGFYPDESVAG"
     gene            5511..5951
                     /locus_tag="VFA02_14280"
     CDS             5511..5951
                     /locus_tag="VFA02_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU11069.1"
                     /translation="MKQLFATSNDFTLTIIRLVLGIVFFIHGAQIGLGWFGGFGWHGT
                     MHFFTSQLGIPAFFAALAILAQLLGGIGLIVGLLSRIAAFGIGIDMLVAIFKVHLHVG
                     FFMNWYGMQKGEGYEYHLLALALCFLVLVKGAGAISLDRLIARD"
     gene            6034..7146
                     /locus_tag="VFA02_14285"
     CDS             6034..7146
                     /locus_tag="VFA02_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11070.1"
                     /translation="MPQSPRRPLSGRPAIAILSLVFSGMLPAFAQHDFNDRDNILIAD
                     QFNNRVIEIERSTHKIVWQFGNGSDKPGPNSIVGTNDAERFGPFTLISGTGIPASNPP
                     LPGCSDPVNGCPDNRVIIVDPHGHILWQYGQDGGVSGSGPDQLNTPVQAVFLSGFPGH
                     PGFFVMITDQANQRVIVVDLFKNIVWQYGTTGVSGSGPNQLNNPNSAELLENGHVLIA
                     DENNNRVIEVTPAGKIVKTFTAGGTISGAAFASRLSNGHTLITDSNNNRAVEVDANDK
                     IVWQYVTNTQPGSNSNPLPTRAIRLRNGDTLISDQFNDRVIEVTMGGRIVFTQGHLNT
                     PGDGFNQLNGPYDAKAIGDFTGLTPPFDFDNDKDDH"
     gene            7288..7569
                     /locus_tag="VFA02_14290"
     CDS             7288..7569
                     /locus_tag="VFA02_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZU11071.1"
                     /translation="MVRHYLVKGRVQGVGFRWYVHREAAAIGLRGWVRNTEDGHVEVV
                     AAGSPEDLDTLRDALRKGSRGSRVDAVIEHELDESEASGLGPFQIEGAW"
     gene            7577..>8028
                     /locus_tag="VFA02_14295"
     CDS             7577..>8028
                     /locus_tag="VFA02_14295"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZU11072.1"
                     /translation="MKHSLDCEPLKALIRTVPDFPKPGILFYDITTLLKDKIGFAQLI
                     DALAAHYIGKEVDLVLGIEARGFIFGPALAYRLNAGFVPVRKPKKLPAQVARVTYDLE
                     YGTDSLEIHLDAIEPGQNVVIVDDLLATGGTMEATVKLVQQLGGHIAG"
BASE COUNT         1794 a         2446 c         2130 g         1658 t
ORIGIN      
        1 cgcgaggtcg gtgaccatct cggacaaatc tacgaaaaac aaggcagaaa gcaggaagct
       61 gcgaatatct acgctttggc cgctgcttcc ggacgcatca cgcctgaccc tgcaggacgc
      121 accacgatgg aagaccgcat ggaccgcctc aaagagcagg gaattcacgc ttcctatcct
      181 gaccctggac gcgaactggg cgagcagcgg tccgtctatc tcccaaaact cacaaagaaa
      241 tacgcttcgg cagaattttt tattgccctc tctccagaca aagtagaaga cgtgcaattc
      301 atccgcggcg acgaagcatt aaaaagcgca ggagacgctc tgcgcaagtc caatttccat
      361 atgccgtttc cgaaagactc aaaggcaaag ctcgtgcgtc gagggattct ggcctgcacg
      421 ccggccatgg aaaagtgcca gttcacgctg ctgctgccac agacaacgac cacgcaatag
      481 tatttttcgc aaatgggtgg tccgtcattc tgacgaccgt agggaggaag aatcttagcg
      541 atgcaaggct ttagctagtt acgatgattt caaccgcttg atctcccaga ccatcacttc
      601 cggtgcgggc cagaattatt cctgggtcta tgaccgttat ggcaaccgct ggcaggagaa
      661 cccgaacggc gcccaaatgg tgttcaacaa agccaataac caaatcacga cgtcgggtta
      721 tagctacgat gcggccggaa acctgatctc cgatggcgtg aatccataca gttatgatgc
      781 ggaaggcaat ctggtgcagg agatcacagg gtcaggcacg gtgaagttca gctacaatgc
      841 tctcaaccag caggtgcgga ccgactggct gagcagcggg tcggcggccg aagtggcctt
      901 caatttgtcg ggccagttgg cctctctgtg gtgggccagc gacggcagcc tgcttttagg
      961 caaggcgtat ctggggagca taccgctgga atcgtatctg ccgcggacaa acatggcata
     1021 tttccagcac cgggactggg agaacacaga gcgcgtgatc acggatgcga gtggaaatgt
     1081 gaccaacatt cgcatttcgc aaccctttgg cgatggcgct gcaaacacga ccgggagccg
     1141 tgacaatacg tatgatggct tcaccggact gtgggacgga gcagccagct ccaccaacca
     1201 tgcccagtat cgggaatact cgaacatcga aggccgctgg ctgtcgcctg atccctacga
     1261 cggcagctat gatctcacca atccccagag cttcaaccgc tacagctatg tggggaacaa
     1321 tccgctgggg tttgtggatc cgagtgggct ggatggaaag tcctcaacga tcaccacagg
     1381 gggatgcaca ggcgcgatta tgtctggcgg cgctgcatgg ggtgctgatt tggcatgttt
     1441 gtttggctta ctccatctgt ttggagggcc gagcttccac ggaacactaa gaccgcgtcc
     1501 ggaaggcact ggaaatccgg actgggccaa cgtggatggc agtctgggag agacactggg
     1561 gttgcccagc gacataaact ggagcaaaat caatccagga atcgcaggcg cgctcggcct
     1621 gcctgacgct ggctgcgagt tcggaagctg cggcgatatc ggctctattg cctcatcatt
     1681 cacgcagggg gcagctatcg cagcgccgtt gacctggtgc ggggagcatc cgaccatctg
     1741 caccgcagga gccgatctag cagccttcgt tcgggcgata cctgccgtaa ccgcaagcgt
     1801 tcttttgctc aggatggaag gagatgggcc agcaactgct ccgggagtcg atagagcagc
     1861 gtgctacgag caatacgagc gcgatctgga tgtttgcagg aaaaaccaca agaggtcgtg
     1921 ctacgcccag gctatggagc gctactcagc gtgcctcgcg gggaagtctg cgccaccgtt
     1981 caatttctga aaggaatgac gatggagcat acactcgaag tcattgccga acgcaatctc
     2041 acgctggact cagatggaat cgaaagggtt gttacggtcc aaattggtcg tccctacgtg
     2101 gagcctgacg gggcgtggtt ctgtcccttc aatgtgcttg gccttgctcc ggcagggacg
     2161 aggagggcgg gaggggttga tgcaattcaa gcactccagc ttgctcttgt catgattggt
     2221 gccgaactat ctgcctcttc gaaatcgctt aaatgggatg gggagccttc caccggtttt
     2281 ccggcaagca tccacgatcc ggtattaggg cccgagtagc tgcaagaggc tggggcccgc
     2341 ccgacgcccc tgttgtggat gcgtcccaag aactgcgccg cctgcggcgt cggcagtagg
     2401 gcgtcgcggt agggatacgg ctttcgccgt accccccgca cagatccgga cgggcggatt
     2461 tcccgcatcc ggctcttacc tcaggtggtg acgcgcagac ggcgcgaagg ataaggagag
     2521 acactggggt tgcccagcga cataaactgg agcaaaatta atctaggcat cgcaggtgcc
     2581 ctcggcctgc ctgacgctgg ctgcgagttc ggtgcctgcg gcgcggggcc atccgcgctt
     2641 acgccggggc aaacggcttc acttgccggg ccgatcctga tcccgccatc atggttgctt
     2701 tccgtagtca atctctttca cttcccctat gcggactctt ccgatcctaa ccataggctc
     2761 tttggcacgc attactgtgg gccgggtggc ggtggtgctt cgacaggcgg cctagatcaa
     2821 ttgtgtgcag ctcacgatgc ctgctatcgc gcgaacggca atacccatca gacgtacaac
     2881 atatccctga catgccactg attatgaagc tgcgaattcg cttaatcctg ctgacgctga
     2941 cgtgctgcgc tctcgctggg tgtcatgcca agcagccacc ggttacacgt gaggctcttg
     3001 ttggaagcta tacctacatg tcgaaagacc ctgcaagcac gccgacaaac cacaatcttg
     3061 accacttggt cttgcggaca gatgggacat acgacctcat cgaaggagga acgacgaagg
     3121 ccgtatccga aaagaagggt acgtggcgga tagagcccgg aactcccgcc gatcatgtag
     3181 acgtcgtcct cgacaactct ggctacccgg ttgagatcaa ggggcaagag gtgcggcttt
     3241 tgatcgactt ggacactgga atatggtggg tcaagactaa gtgaggtgcc agcggaggcc
     3301 gccttccggt ggcctgcccc cattctccgc acactgataa tacttagcca gctgcagccg
     3361 tagccatgga aaagtgccag tttacgctgc tgctgccaca gacaacgacc acgcaatagt
     3421 attttttgca aatgtgcagt ccgttattct gacggccgta gggaggaaga atcttagcga
     3481 tgtaaggctt taggccaata ctgctaagat tttccacgcc ccggaatgac gatcattctt
     3541 tttcacagat cacagtgaaa caaaaattgc tttatgtagt ccttatgcag agttcccaac
     3601 ctgtgactcc gcctttaccc ggcaacgctc tcatccggat aaaacccggc aatctccttc
     3661 gcatactttt catagatcac gcgccgcttc agcttcaggc tgggagtaag ttctcctgaa
     3721 gccagcgacc actcttccgg cacaatcctg aagcgcttca tcgtctcaaa atgcgcaagc
     3781 gatgagttca cttcgtttac gatttcctga tagcgcgcca ccaccgcagg atgctccacc
     3841 agttcgcgtc gtgtaggagc agtaatccct tgctggcgtg tccactcttc cagcgcctgg
     3901 aagttcggtg aaatcagcgc ggcaataaac ttgtgtttgt ctcccaccac tgccgcctgc
     3961 ccaaccagca ggttggcctt cagcttgttc tcaatcggct gcggggccac catcttgccg
     4021 cctgaagtct tcagcagctc tttcttccgg tcagtaatgt agagaaatcc gtccccgtct
     4081 atgtgtccaa tgtctccggt gcggaaccat ccttctgcat cgaacgcctc cgcggtttca
     4141 tccggcttat gccaatatcc tgaaaagaca ttcggcccgc gcaccagcag ctcgccatct
     4201 tctgcaatgc ggcattccac attcggcaaa ggcttgccca cagagccgat ccggtacgcc
     4261 gccgtagtgt tgatggcaat cacaggcgaa gtctccgtca atccatagcc ttccaggatc
     4321 cgaatgccca cgcccgcaaa ccagcccgct gtgtccatgc ccagtggagc gccgcccgca
     4381 atgtagtagc gcacgcgtcc gccaaatcca tgctggatct tactgaacac cagcttgttc
     4441 gcaagcttcc agcccagcga agacggcgct tcgccgcgca gaatctcttc gcgatggctc
     4501 tgtccgatct tcactgccca gtgaaagatt ttcttcttcc atccggactg cgccgcctgc
     4561 cgctccgcct cttgccgcac cttttcaaac acgcgcggca ccgccaccag caccgttggg
     4621 cggatgctct gaagtgcgcc gggcagcttc tcaaagctgg ggcaatatgc tactgtcgct
     4681 tgccgcgtgt aaatggcgta atccaggtgc cgcgctgtaa tgtgcgaaag cggcagaaaa
     4741 gaaatgcacg agtcctgctg cgaaatctca aatcctgcca gcgagtagtt cacattctgc
     4801 gcgatgtttc catgcgtcag catcacgccc ttcggctcgc ccgttgtgcc tgaagtgtag
     4861 ataagcgtcg ccagatcatc tggcccgaca tcataggccg cgcgatcaaa agcagcatcg
     4921 cgctccgggc cgcgtgcatc cgcgcccttc agcagcgacg acatcagcac cgcatccgga
     4981 acaccttcat catccataag aatgatgtgc tccagcgctg tcttctcgcg caccgctgcc
     5041 accttctcat actgctgctt cgatgaaacc acggccacgc gcgagcccga atccgccagc
     5101 agtccgccaa tctgctctgc cgtcagcgtg ggataaattg gcacatccac agcacccatg
     5161 gccagcgcag caaagtccgt gacggcccac tcccagcggt tctcagcaag caacgcaatg
     5221 cggtcgccct tccggatgcc ccatccgccc agcgcctcag ccagcgtccg cacgcgctga
     5281 taaatctgcg tcccggtaaa aggttgccag ttgccgtccg catccatata gagcaccgcg
     5341 cgcggattcg cgttcgacgc ggccaaaaac aaaatatcgt tgatcgtttt aagctgaagc
     5401 atccctgctc gaccaatcta tcggagccgc aggcaattgc caagcatcac aaaccccttg
     5461 catttcgcgg cctggtgagc gcactctgtt tcatacctgg agggacaccg atgaagcagc
     5521 tctttgccac gtccaacgac ttcacgctca ccatcatccg gctcgtactc ggaatcgtct
     5581 tctttattca cggtgcgcag attggcctcg gatggtttgg tggcttcggc tggcacggga
     5641 ccatgcattt cttcacctcg cagcttggca tcccggcatt ctttgccgcg ctggccatcc
     5701 tcgcacaact gcttggcgga atcggcctga ttgtcggcct cctcagccgt atcgctgcct
     5761 tcggcattgg tattgacatg ctggtggcca tcttcaaagt ccacctccac gtgggctttt
     5821 tcatgaactg gtacggaatg cagaaaggtg aaggctatga gtatcacctg ctcgcgcttg
     5881 ccctctgctt tctggttctg gtcaaaggcg caggcgcaat ttcactggac cgcctcatcg
     5941 ccagagactg aaatgacaaa gcagtgacaa tcccctgaca aacagtctgg gccgcagagc
     6001 tactcttttg gggcgcgaaa aggagatgtc cgtatgcctc aaagcccacg cagacccctc
     6061 tccggaagac ccgccatcgc cattctcagc ctggtctttt ccggaatgct tccagccttt
     6121 gcccaacacg acttcaacga ccgcgacaac atcctcatcg ccgaccagtt caacaaccgc
     6181 gtcattgaaa tcgaacgcag cacccacaaa attgtctggc agttcggcaa tggatcagac
     6241 aagcccggcc ccaacagcat tgttggcact aatgacgcgg aacgcttcgg tcctttcact
     6301 ctcatctccg gcaccggaat ccccgccagc aatccgccgc tccccggctg ctctgatcca
     6361 gtcaatggat gccccgacaa ccgcgtcatc atcgttgacc cgcatggcca tatcctctgg
     6421 caatatggac aggacggcgg cgtctccggc tccggcccgg accagctcaa cactcccgtg
     6481 caggctgttt ttctctccgg attccccggt catcccggtt tcttcgtcat gatcaccgac
     6541 caggcgaacc agcgtgtcat cgtagtggat ctgttcaaga acatcgtctg gcaatatgga
     6601 acaacaggcg taagcggcag cggcccaaac cagctcaaca atccaaacag cgcagagctg
     6661 cttgagaacg gacacgtcct catcgccgat gaaaacaaca accgcgtcat cgaagtcact
     6721 cctgcaggaa agatcgtaaa gaccttcacc gccggaggca ccataagcgg cgccgccttt
     6781 gccagccgcc tgtccaatgg ccacacgctc attaccgatt ccaataacaa tcgcgctgta
     6841 gaagtagacg ccaacgacaa aatcgtctgg cagtatgtca caaatacgca gcctggcagt
     6901 aacagcaatc cattgcccac gcgcgctatt cgcctgcgta acggagacac cctcatcagc
     6961 gaccagttca atgaccgggt gatcgaagtg accatgggcg gcaggatcgt cttcacacag
     7021 ggccacttaa atacacccgg cgacggattc aaccaactca acggccccta cgacgcaaag
     7081 gccataggag acttcaccgg gctgacacca ccctttgatt tcgacaacga caaagacgac
     7141 cactaagaaa aacacgctgt catcgttgaa gagcgaataa aaaaacgcag gtgccccaca
     7201 tcttcaaaag atgtggggtc gcatgcgccc acggcttgca tccacgccca tcatctgaaa
     7261 aactggcatc ggttccccgt gacttctatg gtccgtcatt accttgtcaa aggccgtgtg
     7321 cagggagtag gttttcgctg gtacgtgcac cgtgaggctg ctgccatcgg tctgcgcgga
     7381 tgggtgcgga acactgaaga tggtcatgtt gaagtcgttg ccgcaggcag ccccgaagac
     7441 ctggacacac ttcgtgatgc cctccggaaa ggctcccgcg gcagccgtgt agatgccgtc
     7501 atcgaacacg aactcgacga gtcagaagcc agcggcctgg gaccatttca gattgaagga
     7561 gcctggtaaa accagcatga aacattctct tgattgcgag ccattgaagg cgctcattcg
     7621 caccgtgcct gacttcccca agccggggat ccttttttac gacatcacca ccttgctcaa
     7681 agacaaaatc ggctttgccc aactcattga cgcgctcgcc gcgcactaca tcggcaagga
     7741 agtagatctg gtcctcggca tcgaggcccg cggatttatc ttcggtcccg cgctcgccta
     7801 ccgtctgaac gcaggcttcg tgcccgtgcg caagccaaag aaacttcccg cgcaggtcgc
     7861 ccgcgtcacc tacgatctcg aatacggcac ggactcgctc gaaatccacc tcgacgccat
     7921 cgaaccgggc cagaacgtcg tcatcgtaga cgacctcctc gccactggag gcaccatgga
     7981 agctaccgtt aagctcgtgc agcagttggg tggccatatc gccgggtt
//
LOCUS       DASRBV010000394         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_753169, whole genome shotgun sequence.
ACCESSION   DASRBV010000394 DASRBV010000000
VERSION     DASRBV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753169"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFA02_14300"
     CDS             complement(<1..506)
                     /locus_tag="VFA02_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU11073.1"
                     /translation="MGTEITLAVLRKQGCRSRRSVHALEWLNFFLADVQTGLGPFLAA
                     YLAASGWSPGRVGLALSLGGIVTVVLQTPAGGVVDAVRRKRATLAIGVGALATGALVL
                     ATNTSVVSVCLAQLLIGGAAPFLAPMLAAMTLGIVGPRCFDRQFGRNQGFNAAGNVFT
                     ALLVAIVSR"
     gene            782..1513
                     /locus_tag="VFA02_14305"
     CDS             782..1513
                     /locus_tag="VFA02_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11074.1"
                     /translation="MFRGMVLAAVLIALQIGDMAILQAQLQPGPDPRPGPHHPATPRQ
                     EAADAIGRAYDALGRDLALTPSTARNVSGVLMQSRDTYQQALSLYQAGDFTGARETAM
                     ASADMAHAVEQLVMADSPGAAPVPAPPQPPAPPGRADTQAAHAYRDLARVRERSARLN
                     NDLDQRASASVAAQVKMLIREAAQLEQRAQSVVANKPEQAGALARAADALLAAADHYV
                     RWAWIANGLVPPAPPPPRPKGPPPP"
     gene            1583..3133
                     /locus_tag="VFA02_14310"
     CDS             1583..3133
                     /locus_tag="VFA02_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU11075.1"
                     /translation="MRRIFPFQANSIKARIVLLAVLTCLLFGAAALGLFLLLRNSEAS
                     VISASERHLTALAQTLALDYANHTQYEAERGVESALSTPGEHGSDEVLSLMTTVVLRR
                     EIGIEGGFYSSKNDDLLGYAFPTHEGPGVKKDIPVTERPTITELARKAASTGQIQTQR
                     YSGARDVIVFVAAPVRVNGSIAGSVWMMQHMQGINTGRSVQILVGTVAFAGAAFACVL
                     LAFLITTRIQSGVSAINTRLNHLQQDLSGPRSAQPQPAEFETVLAGIDTLSMTLREKI
                     ARERELENRLRHQERLSALGQFAAGIAHELRNPLATMRLRAQMTQRADNNAATQKNLD
                     VVLEEISRLDTMIERLLYFSRPIHLSMQPTDLRAFCETILQSWSARFHEAAVDVQCVG
                     AEHLECLVDQDKFRQVFENLISNSLEALRESPNQPGRMTIRFSAEDHFVQVRVEDNGP
                     GLNAETQSKAFDPFFTTKDKGTGLGLSIAYEIVKAHEGELHLENGPSSGATATVRLPR
                     SGYTGTEV"
     gene            3136..4542
                     /locus_tag="VFA02_14315"
     CDS             3136..4542
                     /locus_tag="VFA02_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU11076.1"
                     /translation="MRERILVVDDDNNLRWTLREILEAAGYEVAEAESGRRAFAQLEE
                     KQPDAILCDWKMPEQGGEELLRALMARNEAYHLPVIIMTAHGTGPNAITAMQLGAYDF
                     VTKPFDLDEVLATVKRALQHVCLQREVERLSKQNAGDVSETYERNRSRMVGSSRAMVE
                     VFKAIGRVSRTDVTVLLLGETGTGKELVARTIHENSPRSHRPFVVVNCAALPAELLES
                     ELFGYEKGAFTGALNRKIGKFEHAAGGTVFLDEIGELPLALQPKLLRVLQEHTFERVG
                     GTESIHADFRLIAATNRVLEDEVEEKQFRSDLYYRLKVFSIQLPALRERRSDILPLAE
                     HFLAQFNERNQQRVPGFTEDAIILLQMYSYPGNIRELEHIVERAAVQAAGRAITRELV
                     QQALPSAVSPATDGSLSELLRLPFHASVAAWEKRLIETALRESGGNKADAARKLQIHR
                     RLLYEKMEQLGIDKESPD"
     gene            5068..>5490
                     /locus_tag="VFA02_14320"
     CDS             5068..>5490
                     /locus_tag="VFA02_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11077.1"
                     /translation="MFLPKVLLISATLSLVCVAGCARQAVAPETPPGPSASATLTPPN
                     PAGSPAPQFQPANYTAEGRIRQLNYAEDGRVDGFLLDNGTLIYLPANFSGAIPPLRTR
                     VQVSGSLHPSVSERTVVTAQLVVPTTGSRPGTVTASASA"
BASE COUNT         1238 a         1603 c         1521 g         1128 t
ORIGIN      
        1 cggcttacta tcgccacaag aagcgcagtg aagacatttc ctgcagcgtt gaatccctga
       61 tttctgccga actgccggtc gaagcagcgc ggccccacaa tgccgagggt cattgcggcc
      121 agcatgggtg ccagaaacgg agctgcgcca ccgatcagaa gctgagcaag gcacacagag
      181 actaccgagg tatttgtcgc caacaccaga gcgcctgtgg cgagcgcacc aactccaatg
      241 gcgagcgtgg cgcgcttccg gcgcaccgca tccacaacac cgcctgcagg agtctgtaaa
      301 accacggtca cgattccacc caggctgagg gcaagaccga cacggccggg actccatcca
      361 ctggcagcaa gataggctgc gagaaagggg ccgagtcctg tctgcacatc ggcaaggaag
      421 aagttcagcc actccagagc gtggacgctg cggcgcgacc tgcatccttg cttacgaaga
      481 acagcgagag tgatttccgt gcccacgcac aaggaatggc aacttcatgg ccatttggct
      541 ttggataagc gtctacacag atgcaaagac ttagcatgga gatggaagag cattctgtac
      601 ggaagccgcg cactcaggtg tccgcaaagc gtacaaagac ggtttcagat ttgtcactcc
      661 cagagaggcg gcattcgtgc tgtgctgctg cattttcagg agaatggatg atctccattg
      721 cgaattgccg tctggcagcg tagttgctct tattgaattg gattcgctca agaggagcag
      781 catgtttcga ggaatggtcc tggccgcagt tcttatcgcg cttcagattg gagacatggc
      841 gattttgcaa gcccaactgc aaccaggtcc cgatcctcga ccaggcccgc atcatcctgc
      901 cacgcctcgg caggaggcgg cggatgccat cgggcgcgca tatgatgctt tgggaagaga
      961 cctggcgctg acaccgagta cggcacgaaa cgtcagtgga gtactcatgc aaagccgtga
     1021 cacgtaccag caggcgttga gtttgtatca ggcaggcgat ttcacgggag cacgcgaaac
     1081 cgcaatggcc tctgccgata tggcccatgc tgtggaacaa ctggtgatgg ctgattcgcc
     1141 cggcgcagcg ccggttcctg caccacccca accaccggcg ccgcctggtc gagctgatac
     1201 gcaggccgcg catgcttaca gagacctggc acgcgtccgg gaacgctccg cccggctgaa
     1261 caatgatctt gaccagagag cgtctgcttc cgttgccgcc caggtgaaga tgcttatacg
     1321 cgaggccgcg cagttggagc aacgggcaca aagcgtcgtg gcaaacaaac cagaacaagc
     1381 aggggccctg gcgcgcgcag ccgatgcatt attggccgcg gcggaccatt atgtaaggtg
     1441 ggcctggatt gcgaacggac ttgtgccacc ggcaccgcct cctcctcggc cgaagggacc
     1501 accaccaccg taggagtgca gcccattacc agcaccatgc agatgcggca aaaagcagga
     1561 ggacaaacgc caggccacaa ccatgcgcag gattttccca tttcaagcaa acagtattaa
     1621 ggcgcggatc gtgctgcttg ctgttctcac ctgccttttg tttggcgcgg cagcgctggg
     1681 gcttttcctg ttgctgcgca actcagaagc atctgttatt tccgcttcgg aacgtcacct
     1741 cacggcgctc gcgcagacat tggccctcga ctatgccaac cacactcagt acgaggcaga
     1801 acggggagtt gaatccgcac tctcaactcc cggagaacac ggttccgatg aagtcctttc
     1861 cctgatgacg acggttgtcc ttagacgcga gattggcatc gagggcggct tctactcgtc
     1921 aaaaaacgac gaccttctcg gttacgcttt tccgacgcat gaaggaccgg gagtgaagaa
     1981 agacatcccc gtaactgagc ggcccacgat taccgagctt gcccgcaagg ccgcctcaac
     2041 cggccagata cagacgcaac gctactccgg cgctcgtgac gtgattgtct tcgttgcggc
     2101 ccctgttcgg gtcaacggaa gcattgcagg cagcgtctgg atgatgcagc acatgcaagg
     2161 catcaacacg ggaagaagcg tgcagatcct ggtgggcaca gttgccttcg caggagcagc
     2221 ttttgcctgc gtcctgctgg ccttcctcat tacgacgcgt atccagtcgg gcgtaagtgc
     2281 aatcaacacg cggctgaatc atttacaaca agacttgtcg ggaccgcgtt ccgcgcagcc
     2341 gcagcctgcc gagtttgaaa ccgtgctcgc cgggattgac acactctcca tgaccctgcg
     2401 cgagaagatc gcccgggagc gcgagcttga aaacagattg cggcatcagg aacggctctc
     2461 cgcactcggc cagtttgccg cgggcattgc ccatgaactc cgtaatccac tggcaaccat
     2521 gcgtctgcgg gcccagatga cccagcgggc tgacaacaac gctgcaaccc aaaagaacct
     2581 tgacgtggtc ctcgaagaaa tctcacggct cgataccatg attgaacggc tgttgtattt
     2641 ttcccggccc attcatctgt ccatgcagcc gacggacctc cgtgccttct gcgaaactat
     2701 actgcaatcg tggtcagcgc gttttcacga ggccgctgtg gacgtgcaat gtgttggcgc
     2761 ggaacatctc gagtgcctgg tggaccagga caaattccgg caggtatttg aaaatctgat
     2821 ttcgaactct ctggaagccc tccgtgaaag cccgaatcag ccaggaagaa tgacaattcg
     2881 cttttccgcg gaagaccatt ttgttcaggt ccgggtggaa gacaatggac ccgggctgaa
     2941 cgcagagacc caatcgaagg cattcgaccc gttcttcaca accaaggaca agggaacggg
     3001 tctagggctc tcgattgctt atgagattgt gaaggcccat gagggagaac ttcacctcga
     3061 aaacgggccg agcagtggcg cgacagctac cgtgcgcctg ccccggagcg ggtatacagg
     3121 gaccgaggtt tagccatgcg cgaacgaatt cttgtcgtgg atgacgataa caatcttcgc
     3181 tggacactca gagaaatact cgaagctgcc gggtacgaag tagccgaagc agaaagcgga
     3241 cggcgggcgt ttgctcaact ggaagagaag caacccgacg caattttgtg cgactggaaa
     3301 atgcctgagc agggcggcga agaactcctg cgggcactca tggcgcgaaa cgaagcatac
     3361 cacctgccag taatcattat gacggcccac ggcaccggac ccaatgccat cactgcgatg
     3421 cagttaggcg catacgactt cgtcaccaaa ccgttcgatc tggatgaagt gctggccacc
     3481 gtgaagcgtg ccctgcaaca tgtctgtcta cagcgcgaag tcgagcggtt gagcaagcag
     3541 aacgctggtg acgtgtcgga gacctatgag cggaacagaa gccgcatggt cgggtcttcc
     3601 cgcgccatgg tggaggtatt caaagcaatt ggccgtgttt caaggacgga tgtcaccgtg
     3661 ctcctgctcg gagagaccgg caccggtaaa gaactcgtcg cgcgcaccat tcacgaaaat
     3721 agccctcgca gtcatcgtcc ctttgtcgtt gtgaactgcg ctgcacttcc tgctgaattg
     3781 ttggagtcgg agctattcgg ctatgagaaa ggtgcattta ccggcgccct caatcgaaaa
     3841 atcggcaaat ttgaacacgc cgctggcggg actgtttttc tcgacgagat cggcgaattg
     3901 ccgctcgcgc tgcaaccgaa actgctgcgc gtactccagg agcacacgtt tgagcgagtc
     3961 ggcggtacag aatccattca tgcggatttt cgtctcatcg cggcaacaaa ccgggtcctt
     4021 gaagatgagg tcgaggaaaa acagttccgt tccgacctct attaccggct taaggtcttc
     4081 agcattcaat tgcctgcact gcgcgagagg cggtcggaca ttctgccgct tgcagaacat
     4141 tttctcgctc aattcaacga gcgaaaccag caacgcgtcc caggtttcac tgaagacgca
     4201 atcattctgc ttcagatgta ttcctatcct ggcaatatac gggaactcga acacattgtg
     4261 gagcgcgccg ctgtccaggc cgcgggccgg gccatcacac gcgagctggt gcaacaggcg
     4321 ctgccttcgg ctgtttctcc tgcaaccgat ggcagcctgt cggaactatt acggttgccc
     4381 tttcacgcat ccgttgccgc gtgggaaaag cgcctgatcg aaaccgcact cagggagagc
     4441 ggcggaaaca aggcggacgc ggccagaaaa ctccagattc atcgcagact cctttacgag
     4501 aagatggagc aattgggaat cgataaggaa tcacctgatt agtcctgtca ccgcatcctt
     4561 tatctgatcg aaatcctggc aaatggccat ttcggcgatc accgccgtct gccggcccct
     4621 cgtccgcttt tctcacactc gcatgttcgc ttaaaggtcg tctggtggtc tgtctttccc
     4681 ggcctgcctc tatatctctt tgattgacca atctttcacg tttggaaccg gccatgccat
     4741 gtccgacttt cgctgattca gccgattgca catcttcgcc agcgcgatat gtaaccacac
     4801 gctgcggctg cctggatacc agccctatcg gaacgactga aaagatcatt ccctatgcct
     4861 tggcaccgga ccatcgaatt tcgccctcaa tctgcacgtc agcaaggtat tagtgttcgc
     4921 ttttgtcacg cgtcctgttc gcttagcggt cggagttttc ggtcactttc tcacgcaatg
     4981 ctcgtatcct gttgaattgc tgatattgca ttgcgctgct ggtttggcaa ttgccgtgcc
     5041 tctcaatgga tgaatggagt ggcacacatg ttcttaccga aagttcttct catatccgca
     5101 actctcagtc tggtctgcgt cgcagggtgc gcaaggcaag cggttgctcc cgaaacaccg
     5161 ccagggccat ccgcaagcgc cacgctaaca ccgccaaatc cggctggttc ccctgctccg
     5221 caatttcagc ctgcaaacta cactgcagaa gggcgcattc ggcagttgaa ctacgccgaa
     5281 gatggacgag tagatggctt tctgctcgac aatggaacgc tgatttacct cccggccaat
     5341 ttctcaggtg cgattcctcc attacgaaca cgtgtgcagg tttccggatc actgcatccg
     5401 tctgtttcgg aacgaaccgt cgtgacggct caacttgtcg taccaacaac cgggagcaga
     5461 ccgggcacag tgacggcttc cgcctcagcc
//
LOCUS       DASRBV010000395         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_394521, whole genome shotgun sequence.
ACCESSION   DASRBV010000395 DASRBV010000000
VERSION     DASRBV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394521"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFA02_14325"
     CDS             complement(<1..1082)
                     /locus_tag="VFA02_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZU11078.1"
                     /translation="MKKRSLLLFLTLFCILFSAAAQSGINQVAFERAQHLRRGINASM
                     WFAQSPGDYSVERLRTYMTPDDIALIHQLGFDHVRLSIDAQPLLEWLRNPDTDTPFMN
                     ELDHAVQTMLAQKLAVIIDVHPESSFKAQLRQGTESVENFARLWRALAKHFASTDPNL
                     VFFEILNEPEQDDPYRWQGIENYIAEQIRLSAPQHTIIATGAHWSGLDDLLMLGPLAL
                     PNIIYTFHDYEPFPFTHQGATWTMSQVRPLRAVPYPSTPGNVQPNLDQEPTLAGQYWL
                     AEYGLDQWNSQRIDATLAFAEKWSALHHAPVYCGEFGVLRDYADPVMRAQWLHDMRVA
                     LDKHHIGWAMWDYQANFGIVTKKNGKA"
     gene            1142..1708
                     /locus_tag="VFA02_14330"
     CDS             1142..1708
                     /locus_tag="VFA02_14330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZU11079.1"
                     /translation="MAEYSLKLMSIKTLSSREVYRNPWLRLREDEIERNNGMRGIYGV
                     VDKDDCAVIIPIEEDHVYLVGQFRYTVQEHCLELPQGGWETADVKPEELARGELREET
                     GFDAEEMTYLGTMYIAYGFANQKQHVFLARGLKHVGTDFDPEEHDLTLHSFTIAEFEQ
                     MLLEGVIRDCCTLAAWGLYKVWREKQEK"
     gene            complement(1779..>2014)
                     /locus_tag="VFA02_14335"
     CDS             complement(1779..>2014)
                     /locus_tag="VFA02_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZU11080.1"
                     /translation="HGIRAVAATIENGTPAHETDFTQPTALFIGNEGNGLPENILRAC
                     DARITIRCPGPVESLNAAIATSILLYEASRQRG"
BASE COUNT          474 a          510 c          587 g          443 t
ORIGIN      
        1 gctttgccat tctttttcgt aacgatgcca aagtttgcct ggtagtccca catggcccat
       61 ccgatatggt gtttgtccag ggccacgcgc atatcgtgca gccattgcgc gcgcattacg
      121 ggatcggcat agtcgcgcag cactccgaac tcgccacaat ataccggagc gtggtgtagc
      181 gcggaccact tttcagcaaa agcgagggtt gcatctatgc gttgcgaatt ccattggtcc
      241 aggccatatt ccgcaagcca gtactgacct gccagcgtcg gttcctggtc aaggtttggc
      301 tggacatttc cgggcgtcga gggatagggg acagcacgca agggacggac ctgcgacatg
      361 gtccacgtcg caccctgatg cgtgaatgga aatggctcat aatcgtggaa ggtataaatg
      421 atattgggga gtgcaagcgg ccccagcatc aataaatcgt caagtccgga ccagtgcgct
      481 cccgtggcaa tgatggtgtg ctgcggagct gaaaggcgga tctgctctgc aatatagttc
      541 tcgattccct gccagcgata agggtcgtcc tgctccggct cgttcaggat ttcaaaaaag
      601 accagattcg ggtccgtaga cgcaaagtgc tttgccagcg cacgccaaag ccgggcaaaa
      661 ttctctacgc tctctgtgcc ttggcgaagc tgcgctttga agctactctc aggatgaaca
      721 tcaataatga cagccagctt ctgagcgagc atggtttgga ccgcatgatc gagttcattc
      781 atgaatggag tgtctgtatc agggttgcgc agccactcaa gaagcggctg tgcgtcaatg
      841 ctcagacgga catgatcgaa gccaagttga tggatgaggg caatgtcgtc gggagtcatg
      901 taggtgcgca gccgctcgac agagtaatcc ccgggcgact gtgcaaacca catactcgcg
      961 ttaataccac gccgcagatg ctgggcgcgt tcaaaggcaa cctggttaat accggattgg
     1021 gccgcagcag aaaacaagat gcaaaacagc gtcaaaaaca gcagaagact tctctttttc
     1081 atcagagatt gctccatttt gggccagagt ttgtggtggt tcttcgcaga ttcaatcttg
     1141 catggcagaa tacagtctga aactcatgag catcaagaca ctcagcagcc gtgaagtcta
     1201 ccgcaatccc tggctccgcc tgcgcgaaga tgaaattgag cgcaataacg gcatgcgcgg
     1261 tatttacgga gtggtagaca aagatgattg cgccgtgatc atccccattg aggaagacca
     1321 cgtttatctt gtgggccagt tccggtatac agtccaggag cactgtctgg agcttccaca
     1381 agggggttgg gaaacagcgg atgtaaaacc cgaggagctg gcgcgggggg aactgcgcga
     1441 ggaaaccggc ttcgatgctg aagaaatgac ttatctcggc acgatgtata tcgcctatgg
     1501 ttttgcaaac cagaagcagc atgtttttct tgcgcgcggg ctgaagcatg taggaacaga
     1561 cttcgatccc gaagagcacg acctgacgct gcactccttt accattgccg aatttgaaca
     1621 aatgttgctg gaaggtgtca tccgcgactg ctgcacgctt gcagcctggg gattgtacaa
     1681 ggtctggaga gaaaagcagg aaaagtgatc cggtcaacgg cctgagagaa agtggactgc
     1741 tgctccgcac tacccgggcc aggtgtgcgg ccgtccggtc agccccgctg gcgcgaagct
     1801 tcgtacagca ggatgcttgt tgctatggca gcattcagcg attcaaccgg gccggggcag
     1861 cggatagtaa tgcgcgcatc gcaggcgcgg agaatattct ccggcagacc attgccttcg
     1921 tttcctataa acagggccgt tggttgggta aaatccgttt cgtgtgccgg ggtgccattc
     1981 tcgatggtgg cagcgacggc gcgaattcca tgat
//
LOCUS       DASRBV010000396        12595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_143662, whole genome shotgun sequence.
ACCESSION   DASRBV010000396 DASRBV010000000
VERSION     DASRBV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143662"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFA02_14340"
     CDS             complement(<1..320)
                     /locus_tag="VFA02_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11081.1"
                     /translation="MRSRLFALVLLFCVSPAIHAVELRISAQALEHTLKTQLFNGPDG
                     RYYIKGDIHSACYVYADSPKVSFSGDRIFVHVHTSARLGTHIRGACIGIGLSPESDVS
                     LLPDA"
     gene            complement(370..729)
                     /locus_tag="VFA02_14345"
     CDS             complement(370..729)
                     /locus_tag="VFA02_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11082.1"
                     /translation="MKKQRSYQPKFARVSEEMRRLAVLLEEEVLRWPDVSVKPMFGMR
                     AIYRNKTIFGLLPETKMINRAGAIAYKFADKPQEKRQGHQWQLFDIEDESQISEALQI
                     LDEAYRKAKVHPSGGRK"
     gene            complement(792..2222)
                     /locus_tag="VFA02_14350"
     CDS             complement(792..2222)
                     /locus_tag="VFA02_14350"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZU11083.1"
                     /translation="MSNELRSFLSLSYAELEEKNLQAKEQRKKRVDPGKIQEERLKYL
                     TDTKEIKAVTVLFSDLEGRLHMLDYDKKFLIKSYDNLTFDGSSIRGFTAQRESDLRLG
                     IDWGSFYWAPADVFGPGKVLVFGDVIDKGGTPYAADVRGLLKGFAEGLHAKHGYTLNA
                     ANEIEGFIFAGTDAERRFPETGRFDYVNTGGYYHSLPGDPLRTFIDTTAEVLRAMGFE
                     NEKDHPEVAPSQFEINYGYGEVLGAADQIQLYKLVCRQVATKLGMTASFLPKPVVGVN
                     GSGMHTNVSISKDGKNLFWDPKGEEKLSKFGWDFVDRILTHGNDICLLLNSSVNAYRR
                     LDPHFEAPNQIKASATDRGSMVRIPIGNEKSMRVEVRSVAPDANPYLVLYSVFKSGLE
                     GETASIANLRQAERYLPDNIYTAIDNFRNATWTTKLLGEDVKARYADLKQASADRCPR
                     LLGTFVKAPEVQFHHEVYNQFLWNQF"
     gene            2495..3463
                     /locus_tag="VFA02_14355"
     CDS             2495..3463
                     /locus_tag="VFA02_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZU11084.1"
                     /translation="MKSKLLIFMMLLVLPRLFAQAAPEKVILDTDIGDDIYDAFAVTL
                     ALASPELKVVGITSAWGDTELRSRMLDRLLCEAGRSDVPVLTGVKTESKTPFSQEAWA
                     HAGEARPHHNAVDFLLDEIRKNPGEITLIAIAPLTNIGAAMDRDPATFRKLKRVVMMG
                     GSIRRGYGDLGFAPAHGPDAEYNIAMDVAAARKLFQSGVPVYMMPLDSTQLAFDETKR
                     EMLSMVSTPLTDSLQVLTAEWQRVTHQITPTLFDVMAVAYAIKPELCPATAMHIDVDG
                     AGFTREVPGTPNANVCLESDSDRFFQFLMPRLLGQRLQGSSSCVGK"
     gene            complement(3522..5522)
                     /locus_tag="VFA02_14360"
     CDS             complement(3522..5522)
                     /locus_tag="VFA02_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZU11085.1"
                     /translation="MPPISACLRILAVALLPCGPMFAQNTPHHKTATKTDSSRPLSNE
                     EKAQHILDRFTFGPRPGEVSVVTKEGWEAWFERQLNPDSIPDSELDKRLAAYPALNMT
                     PTEIAASFPDGQMLRQIEMGKQSMPKDPILAGAFEVALAKYDQRQAKKNDNQAPPPID
                     MMKPEQPMGRQEQFASAQMLASQILGYPKNMRMQAIMKLPVEQRIVLCRDITEPQRSM
                     LLNDFTPHEREIFYMMAGGGGGGLGVVQNEMMQAKLLRAILSERQLQEVMTDFWFNHF
                     NVFMHKNGFETYYLPDYERNAIRAHALGKFKDLLLATAEHPAMLDYLDNWTSIGPDSP
                     AAQNQRRRGKQAERGLNENYGREVMELHTVSVNGGYTQADVTNLAKIFTGWTIDHPQL
                     GGPFVFDERRHEPGPKKWFGHTVKEDGFNEGVQALMWLAAQPQTAHFISYKLAQRFVA
                     DDPPPALVDRMAKTYLSSDGDIKEVLRTMVHSPEFWSRTYYRNKVKTPLEFVASALRA
                     TDTNPTNPGALVGTIARMGEPLYQMQPPTGYPMTADHWMNSAALVDRLNFSVALANSR
                     LGGMKFDAPHLLAAGLLTHSDATREIPHTEPVSLMQSTTHASGEDEALALMEQLLVAG
                     DVSAKTNTVIKQELAQSQQQDPAQTLDTMTAMILGSPEFQMR"
     gene            complement(5562..6215)
                     /locus_tag="VFA02_14365"
     CDS             complement(5562..6215)
                     /locus_tag="VFA02_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZU11086.1"
                     /translation="MSKRLIIFLKVLVHIACLAPITWLVFQFWRASTTVPDALGPDPI
                     AAVTFFTGKGTLRLLVITLAITPIRKFFPKLGWTISFRRMLGLYAFFYACLHLTTYVW
                     LYSNFSLSTMLDDIGQRRFITAGLAAWLLLLPLALTSTAWAIRKLGGKRWRMLHSLIY
                     VAAIAGVVHYWWGVKTGVRTPLAITVVLGILLLIRVVFHFVSRRNKPKPQMAQPAQL"
     gene            complement(6295..7236)
                     /gene="msrP"
                     /locus_tag="VFA02_14370"
     CDS             complement(6295..7236)
                     /gene="msrP"
                     /locus_tag="VFA02_14370"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZU11087.1"
                     /translation="MLIRKPNDIPSSEITPKTVYLNRRQFMAAATAAAAGAAFTKAGA
                     ETTKLTTIKSPLSTTGEDLTPFDYITHYNNFYEFGVEKGDPAKNAWRLQTRPWTVTVE
                     GLVNKPRTFGIDDLLKLRPLEERVYRMRCVEAWSMVIPWVGYSLSELIKQCEPLGKAK
                     YVQFLSLADRKQMPGIGSVNLNWPYSEGLRMDEAMHPLTLLCFGLYGQVLPNQDGAPV
                     RVVVPWKYGFKSAKSIVKIRFVEKQPHTAWNDQAPDEYGFYSNVNPNVDHPRWSQKYE
                     RRIGEPIFKQRRPTLMFNGYADQVASLYNGMDLRRYY"
     gene            complement(7406..7846)
                     /locus_tag="VFA02_14375"
     CDS             complement(7406..7846)
                     /locus_tag="VFA02_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11088.1"
                     /translation="MLFVLVSRVYDRWAEHLTGLPTTHIAKILPPFGRQNDGLYIARQ
                     NERTLHIQKVLKIRELSLLISHGVRIHFLFLAKLSPAPIALIPCPLQDAGNTPHDRVF
                     PVGTFPFFLNTRARLRARGQNGFALSAAQPGQWPAKVSCQGQPG"
     gene            complement(7827..9209)
                     /locus_tag="VFA02_14380"
     CDS             complement(7827..9209)
                     /locus_tag="VFA02_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU11089.1"
                     /translation="MTSQLKRQLGLGNAISLNMMNMIGVGPFITLPLVVAAMGGPQAM
                     LGWVLGALIALCDGLVWAELGAAMPEAGGSYAFLREMYRREGAGRWLSFLYIWQLGFS
                     APLSIASGCIGLAQYAAYLWPSLNNAVPLLPQLRYASLAAAGTCLFVLALLYRNIRAI
                     ARLAWVLWAGVLFTIGSVILAGTTHFHASQLAIPQGAFHLTPAFFQGLGAATLIATYD
                     YWGYYTVCFLGGEARDPGRTIPRAVLLSIAIVAALYLAMNISVLGVIPWQELVKQGDG
                     RYAVVAVLMQRTFGAAAARILAVLVMWTAFASVFSLLLAYSRAPYAAALDGNYFRSFA
                     RLHPRHGFPNVSLLALGIVATVFCFFDLSRVIAALVAIRIILQYVLQQTGVILLRLRR
                     PEMPRPFRLWLYPLPPLFALAGFLFILFSRRDASREFIYAGVIAVTGSILYFARAYKR
                     REWPFALRSG"
     gene            9276..12116
                     /locus_tag="VFA02_14385"
     CDS             9276..12116
                     /locus_tag="VFA02_14385"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZU11090.1"
                     /translation="MQTARRRLKQIATITLATALTVQAQTVPWATQIAATPDAQTLPE
                     DRGADGLWQTLRKLQTWASMMMIVAHPDDEDGGMLTLESRGAGARVAMLTLTRGEGGQ
                     NAMSSETYDALGLVRTNELLRADEYSGTEQYWTRVADYGFSKTIDEAFQQWGHDRVLY
                     DVVRAVRLNRPLVVTSVFTGNVTDGHGHHQVSGEMAQEVFKAAGDPNVFPDQIAEGLR
                     PWSPLKVYARVPTFSISGEGANKRMFDYATGKSAPLHLRDYVNDQWMDDVPETNVEIP
                     EGAFDPVLGRSYLQMAREGWALQKSQNGGGNPPLPGPFNVAYHRYGSRVQTGEHESSF
                     FDGIDVSLMGIASLAHGDTQFLKDGLREINSDVTRALYSYIPSDPSKILPALHDGYLK
                     TMQLIAAVDASSLSADDKANVNHELQIKLTQFNTALAEALGLQVNALVTPSLQSDRNP
                     FFGSAAQMTFLHATPGMDFHVRLHVSSAGPWTPAGKLRLAKTWLVTPEGENWPVERMA
                     GPTTPATSDADILFKVHVPRNASVTRPYFSRPSIEQPYYNINDKRWLNRSFAPYPVEG
                     WAEFDYDGVPVRVGQVVQTVHWEHGPGAVYQPLVVLPQISVSFGTAAGVIPLGEKSFP
                     LHVTLTNEQQDSADGELHLELPAGWTTGPATAKFHLPANDPVTVTFTVYPTALSSDAA
                     YNIRAVARSGNYEFSESVQQVGYPGLRPYYYYRPSTYRARAVDVKVAPGLHVGYIMGT
                     GDEVPEALEQIGIDPHLLTSDEILHGDLSQYDAIVLGIRTYAARPELAQATGRLLDYV
                     KNGGTVIVQYNSPEYDHNYGPYPYTLGRSPEKVVDEKSQVTILDPSHPLMTWPNHITE
                     ADFNGWVEERGHDFMESWDPHYTALTETHDPGQDPQKGGLLYAHYGKGAYIYVAYALY
                     RQMAEAVPGAYRIFANLVSAGKDHMAQRSARP"
     gene            complement(12149..>12595)
                     /locus_tag="VFA02_14390"
     CDS             complement(12149..>12595)
                     /locus_tag="VFA02_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HZU11091.1"
                     /translation="EPQWAVVGDTFPVGCAWADEIVFREYFGANPDSKVPEYQTEYGI
                     YEPHCGLDNVHLSWGHDEYIYHVAKPYLPEPALYMLRYHSFYPAHKHGAYKHLMNEHD
                     EEMFEWVRKFNPYDLYSKGHTKPNLKELKPYYDDLIAEYFPEKIDW"
BASE COUNT         2854 a         3393 c         3607 g         2741 t
ORIGIN      
        1 gcatccggca gcagggaaac gtcggactca ggcgacaggc cgattccaat acaggctccc
       61 cgaatgtgtg tgccgagcct ggcgctcgtg tgtacgtgga cgaagatgcg gtcgccggag
      121 aaagagactt tcggcgaatc agcataaaca tagcaggcag aatggatgtc gcccttgatg
      181 taatagcgtc cgtcgggccc gttaaaaagt tgcgtcttga gtgtgtgctc aagggcctgg
      241 gctgaaattc tcagttcaac agcatgaatg gcaggagaaa cacaaaacag caggaccagt
      301 gcaaacagac ggcttctcat aaatgatttt attttaggga tttctgcgcc cctggaatgc
      361 aaaaaacggc tacttcctgc cgcctgaagg atgcactttt gccttcctgt aggcttcatc
      421 gaggatttgc aacgcttctg agatctggct ttcgtcttct atatcgaaaa gttgccactg
      481 atggccttgc cgcttttcct gcggcttgtc ggcgaattta taagcgattg cgccagcacg
      541 gttgatcatc ttcgtctccg gcagaagccc aaagatggtc ttgttgcgat agatggcccg
      601 catcccaaac atcggtttta ctgaaacatc aggccagcgc aggacttctt cttccagcaa
      661 cacagcaagg cggcgcatct cctctgagac gcgggcaaac tttggctgat aggatcgctg
      721 tttcttcata aaaacattgc cccggcagta taccggggca gaaagcaaca acgcagtcta
      781 ttgtggttta gttagaactg gttccagagg aactggttat agacctcatg gtggaactgg
      841 acttccggcg ctttgacgaa ggtcccaagc agacgcgggc agcggtcggc tgaggcctgc
      901 ttcaggtcgg cgtagcgggc ctttacgtct tcgcccagca gcttcgtggt ccaggtggcg
      961 ttccggaagt tgtctatggc tgtataaatg ttgtccggca gatatcgctc tgcctgacgc
     1021 agattggcaa tgctcgcggt ctcgccttcg agtccggact tgaagaccga gtaaagcacc
     1081 aggtagggat ttgcgtccgg agcaaccgag cgcacctcga cacgcatgct cttttcgttg
     1141 ccgataggga tacgcaccat ggagccgcgg tctgtggccg acgccttgat ctggttcggg
     1201 gcctcgaagt gcggatcgag acggcgatag gcattgacgc tcgaattcag cagcaggcaa
     1261 atgtcattgc cgtgcgtaag aatgcggtcc acaaagtccc atccaaactt ggagagcttc
     1321 tcttcgccct tggggtccca gaagaggttc ttgccgtctt tgctgatgga gacattcgtg
     1381 tgcatgccgc ttccgttgac gccaaccacc ggcttaggca ggaacgatgc agtcatgccg
     1441 agtttggtgg ccacctggcg acagaccagc ttgtaaagct gtatctgatc ggcagcgccc
     1501 agcacttcac cgtaaccgta gttgatctca aactgcgaag gcgcaacttc ggggtggtct
     1561 ttctcgttct caaagcccat ggcgcgcagt acttcagccg tcgtatcaat aaaggtgcgg
     1621 agcgggtcgc cggggagcga gtggtagtaa ccgcctgtat ttacatagtc aaacctgccg
     1681 gtctcaggga agcggcgctc ggcatcggtc ccggcaaaga tgaagccttc aatttcgttg
     1741 gcggcgttga gtgtgtatcc atgctttgca tggaggccct cagcaaagcc cttcaaaaga
     1801 ccgcgcacgt cggcggcata gggtgttccg cccttatcaa tcacatcgcc aaaaacgagc
     1861 accttaccag ggccgaagac atctgcgggt gcccagtaaa aggaacccca gtcaattccc
     1921 agacgcaggt cgctctcccg ctgcgcggta aagccgcgaa tcgaggagcc gtcaaaagtc
     1981 agattgtcgt agctcttgat caggaacttc ttgtcgtagt cgagcatgtg caggcggcct
     2041 tcgaggtcgc tgaacagcac agtgacggcc ttgatttctt ttgtgtcggt gagatacttg
     2101 aggcgctctt cctggatttt tccggggtct acgcgctttt tgcgctgttc tttggcttgc
     2161 aggttttttt cttccagctc cgcatatgag agcgagagga aactacgaag ttcgttggac
     2221 atgcttctcc tctggatggg gttcaaccga agcccaccgt ggcccaacct gcccggaaca
     2281 ggcaggcaag ctcgacctgg ctcgatggtt tttctctgcc aacagggtac cgcgagcagt
     2341 ataagaaatt tttaatatca gaaaaagtga tagcgaggat agaaaaactt tattaagttg
     2401 gccagcccgc gcccgaaaat atgaagatgt cgaagtcaaa gcaagataag attgggcccc
     2461 tgccccagga cccttccctt agaagaaagg aaccatgaaa agtaagttgc ttatctttat
     2521 gatgctgctg gtgttacctc gtctgtttgc acaagctgcg ccggagaagg tcatcctcga
     2581 tacagacatc ggtgacgaca tttatgatgc ctttgcggtg acgctggcgc tcgcgagccc
     2641 ggaattaaag gtcgtcggga ttacgtcggc ctggggcgat acagaactgc gcagccgtat
     2701 gctggaccgt ctgttatgcg aggcgggacg cagcgatgtc ccagtgctta ccggcgtaaa
     2761 gacggagagc aaaacgccat tttcccagga ggcttgggca catgccggag aagcacgtcc
     2821 gcatcacaac gcggtagatt ttctcctcga tgaaatcaga aaaaatccag gagagattac
     2881 cttgattgcg attgctcctc tgacgaatat tggcgcggcg atggaccgcg accccgcgac
     2941 tttcaggaag ctgaaacgtg tggtgatgat gggcggctct atccggcgcg gatatggcga
     3001 cctcggattt gctccagcgc atggacccga cgccgaatac aacattgcca tggatgttgc
     3061 tgcggcacgg aaattgtttc aatcaggcgt acctgtctac atgatgcctc tggattcgac
     3121 ccaactggcg tttgatgaaa cgaagcgcga gatgctgagc atggtaagca ctccgctgac
     3181 ggactcactc caggtactga ccgccgaatg gcagcgggtg acccatcaaa ttaccccgac
     3241 actgttcgac gtgatggctg ttgcgtatgc cataaagcct gagttgtgtc cggcgactgc
     3301 gatgcacatt gatgtggatg gagcaggatt tacccgggaa gttccgggca caccgaatgc
     3361 gaatgtatgt cttgagtcgg attcagaccg gttctttcag tttttaatgc cgagattact
     3421 gggacagcgg ttgcagggaa gttcgtcatg cgtggggaaa tgaagtgtct ggagcgggca
     3481 cccgccatcc tgcaatccaa agtctcgcag cagaagtcta ttcatctcat ctgaaattct
     3541 ggcgagccaa gaatcatcgc agtcatcgta tccagtgttt gcgctgggtc ttgctgttgc
     3601 gattgcgcaa gctcctgttt gatgactgtg ttggttttgg ctgagacgtc tccggcgaca
     3661 agcaactgct ccatcagagc gagcgcttca tcttcaccgc ttgcgtgggt cgtggactgc
     3721 ataagagaga ctggttccgt gtgtggtatc tcccgagtgg cgtcagaatg cgtgaggagt
     3781 cccgcggcca gcagatgcgg cgcgtcaaat ttcatgccgc cgagtctgct gttcgcaagc
     3841 gccacggaaa aattcaggcg gtctaccaga gcagcagagt tcatccagtg gtcggcggtc
     3901 atcggatacc cggtgggcgg ctgcatctgg tagagaggct cgcccatgcg cgcaatggtt
     3961 ccaaccaaag caccgggatt ggtcgggttc gtatcggttg cgcggagtgc cgaagcgacg
     4021 aattccagcg gcgtcttcac tttattgcga tagtacgtgc gcgaccaaaa ctccggcgaa
     4081 tgcaccatgg ttcgcaggac ttctttgatg tctccgtccg aagagagata cgttttcgcc
     4141 atgcggtcta ccagcgcagg tggaggatcg tcggcaacaa agcgctgcgc cagcttgtag
     4201 ctgatgaagt gcgcagtttg tggctgcgcg gcgagccaca tcagcgcctg gaccccttcg
     4261 ttgaagccat cttcttttac cgtgtggcca aaccattttt tcgggccggg ttcatgcctg
     4321 cgctcgtcaa agacgaatgg gccgccaagc tgcgggtgat caatggtcca tccggtgaat
     4381 atttttgcca gattggtaac gtcggcctgt gtgtatccgc catttacgct gacggtatgc
     4441 agttccatga cttcgcgccc gtagttctca ttcagcccgc gttcggcctg ctttccacga
     4501 cggcgctggt tctgtgcggc gggtgagtct ggtccgatgc tggtccagtt gtcgagatag
     4561 tcgagcatgg ccgggtgctc ggccgtggcc agcagaaggt ctttaaactt gcccaaggcg
     4621 tgtgcccgaa tggcattgcg ttcgtaatca ggcaggtaat atgtctcaaa gccgttcttg
     4681 tgcatgaaga cattgaagtg gttgaaccag aagtcggtca tcacctcttg aagctggcgc
     4741 tcactcagaa tggcgcgcag aagcttcgcc tgcatcattt cattctgcac aacaccgagg
     4801 ccgccgcctc caccacctgc catcatgtaa aagatttcgc gttcgtgcgg agtgaagtcg
     4861 ttaagcaaca tgctgcgctg tggctcggtg atgtcgcggc aaagtacgat gcgctgctcg
     4921 acaggcagtt tcatgatggc ctgcatgcgc atgttctttg gatagccgag aatctggctg
     4981 gcgagcatct gcgcagaagc gaattgttcc tgacggccca tcggctgctc aggcttcatc
     5041 atgtcaattg gaggaggggc ctggttgtca ttcttttttg cctgccgctg gtcgtatttt
     5101 gcgagtgcca cttcaaaggc cccggcgagg atggggtctt ttggcatgga ctgcttgccc
     5161 atctcaatct ggcgcagcat ctgcccgtct gggaagctgg ctgcgatttc tgtaggagtc
     5221 atgttcaggg ccggataagc ggcgaggcgt ttgtctaact cgctatcagg aatgctgtca
     5281 ggattgagtt gccgttcaaa ccacgcttcc catccttctt tggtgactac ggacacttcg
     5341 ccaggacgtg ggccaaaagt gaagcggtcg agaatgtgct gcgccttttc ctcattggag
     5401 agcgggcggg acgagtctgt tttcgtggcc gttttatggt gcggtgtgtt ctgcgcgaac
     5461 attgggccgc agggaagaag cgcaacagca agaatccgca aacaggcaga gataggcggc
     5521 ataacttact cctgacacta ctgtagaccg cagtttacct ctcagagttg cgctggttgg
     5581 gccatttgtg gcttgggttt gttccgtctt gagacaaaat ggaacaccac ccttatcaaa
     5641 agcagaatgc cgagcacaac agtgatcgca aggggcgtac ggactcctgt tttcacgccc
     5701 caccaataat gcacaacgcc tgcaatggcc gcaacataga tgaggctgtg cagcatacgc
     5761 cagcgcttgc cccccagctt gcggatggcc catgccgtcg aggtcaacgc aagcggcaga
     5821 agcagtaacc atgcggccaa tccggcggta atgaagcgac gctggccgat gtcatccaac
     5881 atcgtgctga ggctgaaatt ggagtagagc cagacatatg tcgtcaggtg caggcaggca
     5941 taaaaaaagg cataaaggcc aagcatccgg cggaagctga tcgtccagcc cagttttgga
     6001 aaaaatttgc gaatgggcgt gatggccagc gtgatgacca gcagacgcag cgtgcctttt
     6061 cctgtaaaaa aagtgaccgc cgcaatggga tcagggccaa gcgcgtccgg gaccgtagtg
     6121 ctcgcgcgcc agaattgaaa cacaagccat gtaatgggag caaggcacgc gatgtggaca
     6181 aggactttta gaaagatgat gagccgtttt gacatataaa agcaggtcct tcactccctc
     6241 ttcactacgc tcagggtccg tttcatggag ggcaattctg aaaagcgatg ctgcttagta
     6301 gtaccttcgc aggtccatac cgttgtagag actggccacc tggtctgcgt atccattgaa
     6361 catgagtgta ggacgccgtt gcttgaaaat cggctcgcca atgcggcgct cgtatttctg
     6421 gctccagcgt ggatggtcta cattgggatt cacgttcgag tagaagccgt attcatcggg
     6481 cgcctggtcg ttccaggccg tgtgcggctg cttttccaca aaacggattt tcacaatgga
     6541 ctttgccgat ttgaatccgt atttccatgg caccacgacg cggacaggcg cgccgtcctg
     6601 attgggcagc acctgaccat acaggccgaa gcagagcagc gtgagcgggt gcatcgcttc
     6661 gtccatgcgc aaaccttctg agtagggcca attgagattg accgagccga ttccgggcat
     6721 ctgtttacga tcggccagcg agaggaactg tacatacttt gctttgccca gcggctcgca
     6781 ttgcttaata agttcagaga gggaatagcc gacccacgga atgaccatgc tccacgcctc
     6841 aacgcagcgc atccggtaaa cgcgctcttc cagtgggcgc agtttcagca aatcatcaat
     6901 gccaaacgtt ctgggcttgt tgaccaggcc ctcaacggtc accgtccacg gacgtgtctg
     6961 caagcgccat gcgttcttgg ccgggtcgcc cttctcaacg ccaaactcat agaaattgtt
     7021 gtagtgcgta atgtaatcga agggcgtaag gtcttcgccc gtggttgaaa gcgggctttt
     7081 gatcgtggtg agctttgtcg tttcagctcc ggcctttgtg aatgctgctc cggctgctgc
     7141 cgcagtggcc gctgccataa actgccgcct gttcaggtaa acagtcttcg gagtgatctc
     7201 tgatgaggga atatcgttgg gcttacggat gagcatcgct tgacctgctt ttcttcttac
     7261 agattaaagt acgcttctgg tagcaagcat ggattttcca tgtgctaaaa ccagactctt
     7321 acggccggac tctactcata agacagaaac gctgcggaaa aggttacatt tttccaggca
     7381 ggagtaacaa tcaacaatat ggaaactatc caggttgtcc ttggcaagaa acttttgcag
     7441 gccattgacc gggctgcgcg gcagacaaag caaaaccgtt ctgccctcgt gcgcgaagcc
     7501 ttgcgcgagt atttaaaaaa aatggaaatg tgcccactgg aaagacgcga tcatgagggg
     7561 tattcccggc atcctgcaac ggacacggaa tcaaggctat gggagcggga gatagtttgg
     7621 ccagaaacaa aaaatggatg cgtaccccat gggaaataag caaagaaagt tcacgaattt
     7681 ttagcacttt ttgtatatgc agagtccgtt cattctgacg agcgatatat agtccgtcat
     7741 tctgacgacc aaagggggga agaatcttag cgatgtgcgt ggttggcaat cccgtaagat
     7801 gctccgccca gcggtcatag acacgactaa ccagaacgaa gagcaaacgg ccactccctg
     7861 cgcttgtaag cccgcgcgaa atagaggatg ctccctgtta ccgcgatcac gcccgcatag
     7921 atgaattcgc gcgaagcatc acgtcttgaa aagagaatga aaagaaatcc agccagcgca
     7981 aacagcgggg gcaggggata gagccaaagc cgaaaagggc ggggcatctc cggccggcgc
     8041 agacgcagca ggatcacgcc agtctgttgc agtacatatt gcagaatgat gcggatggcg
     8101 accagcgcag caatcacacg cgagagatcg aagaagcaga aaacagtggc cacaattccg
     8161 agtgccagca gggaaacatt cgggaaccca tgccgtgggt gcagccttgc aaacgagcgg
     8221 aagtaattgc cgtccagcgc tgctgcataa ggcgcccgcg aatatgccag cagcagcgaa
     8281 aaaactgaag cgaaggccgt ccacatcacc agcacagcca gtatccgcgc ggccgctgct
     8341 ccaaaggtcc gctgcataag aacagcgacg acggcatagc ggccatctcc ctgcttcacc
     8401 agttcctgcc acgggatgac gccgagcacg ctgatgttca tggcgagata aagcgctgca
     8461 acaatggcaa tggaaagcag cacggcacgg ggaatcgtgc gtccggggtc gcgtgcttct
     8521 ccgccaagaa agcacaccgt gtaatagccc cagtaatcgt aggtcgcaat cagagtggcc
     8581 gcgccgagtc cctgaaaaaa tgcgggtgta agatgaaatg ctccctgcgg tatagcaagc
     8641 tgagatgcat ggaaatgcgt agttccggca agaatgacgg acccgatcgt gaacaggacg
     8701 ccggcccata agacccatgc caggcgcgcg atggccctga tgttgcgata gagcagcgct
     8761 agcacgaaga ggcatgttcc cgcagcggca aggcttgcat atcgcaattg cggaagcagc
     8821 ggcacggcgt tgttgagcga aggccagaga taggccgcgt actgcgccag cccaatgcat
     8881 ccggaggcaa tggaaagcgg cgcgctgaat ccaagctgcc agatgtagag aaacgagagc
     8941 cagcggcctg cgccttcgcg tctatacatt tcgcgcagaa aggcgtagga gcctccggcc
     9001 tcgggcatcg ccgcgcccag ttcggcccat accaatccat cgcagagggc gatcagcgcg
     9061 ccgagtaccc atccgagcat ggcctgtggc ccgcccatcg cggccacaac cagcggcagc
     9121 gtaatgaatg gtcctacgcc gatcatgttc atcatgttca gcgagatggc gttgcccagg
     9181 ccaagctggc gtttcagttg cgacgtcatg gttcgatatt catctaagaa agaacacagt
     9241 ggatggattg tacactggaa acactagccg aatagatgca gacagccaga cgacgactca
     9301 agcagattgc taccatcacg cttgccacgg ccctcaccgt gcaggcgcag acggtcccct
     9361 gggccacgca gattgccgcc actcctgatg cgcagacttt gccggaagat cgaggcgcag
     9421 atggattatg gcagacactg cgcaaactgc aaacctgggc gagcatgatg atgatcgtag
     9481 cgcaccctga cgacgaagac ggcgggatgc tgacgctcga atcgcgtgga gccggtgcgc
     9541 gcgttgccat gctgacgctc acacgcggcg aaggcggaca gaacgccatg tccagcgaga
     9601 cgtatgatgc actgggtttg gtccgcacca atgagttgct gcgcgccgat gaatacagcg
     9661 gaacagaaca gtactggacg cgtgtcgccg attacggatt ttccaaaacc atagatgagg
     9721 cctttcagca atggggtcat gatcgcgtcc tctacgacgt agtgcgcgcc gtgcgcctga
     9781 atcgcccgct tgtcgttaca tcggtcttta ccgggaatgt taccgatggg cacgggcacc
     9841 atcaggtatc gggcgaaatg gcgcaggagg tctttaaggc cgctggtgac ccgaatgttt
     9901 tccctgacca gattgccgaa ggtctgcggc cgtggtcgcc cctgaaagtg tatgcgcgtg
     9961 tgcccacctt ctccatcagc ggcgaaggcg caaacaagcg catgttcgac tatgcaacag
    10021 ggaagtctgc gccgctccat cttcgtgact atgtgaatga ccaatggatg gacgatgttc
    10081 cggaaacgaa tgtggaaatt cctgagggcg cgttcgatcc cgtgctgggc cgttcctatc
    10141 tgcaaatggc gcgcgaggga tgggcactgc agaaatcgca gaatggcggc ggaaaccctc
    10201 cgctcccggg gccattcaac gttgcttatc accgctatgg ctcgcgggta caaaccggcg
    10261 aacacgagtc gtccttcttt gatggcatag atgtttcgct gatggggatt gcctcgcttg
    10321 cccacggcga cacgcagttt ctaaaagatg gcttgcgcga aatcaacagc gatgtgacac
    10381 gcgctttgta ttcgtacatt ccttccgacc catcaaaaat attgcccgcc ctgcacgacg
    10441 gttatctgaa gacgatgcaa ctgattgccg ctgtggacgc aagttccctc agcgcggacg
    10501 acaaggcaaa tgtcaatcac gaacttcaaa tcaagctgac gcagttcaac accgcactgg
    10561 cggaagcact tgggctgcag gtgaatgcgc tggtcacgcc cagtctgcaa tctgaccgca
    10621 acccattctt cggctctgcc gcgcagatga cctttctcca cgcaactcca ggcatggact
    10681 ttcatgtacg cctgcatgta tcgagtgcag ggccgtggac acctgcgggc aaactccgtc
    10741 tggccaaaac ctggcttgtc actcccgaag gcgagaactg gccggtggag cggatggccg
    10801 ggcccaccac tccagccacg tcagacgcgg acatcctctt caaagtccat gtgccacgca
    10861 acgcttccgt gacacgcccc tatttcagcc ggccttccat cgagcagccc tactacaaca
    10921 tcaacgacaa gcgctggctc aaccgctcct ttgctccgta cccggtcgaa gggtgggccg
    10981 agttcgatta tgacggtgtt ccagtgcgtg tggggcaggt agtgcagacc gtgcactggg
    11041 agcatggacc gggcgcggtg tatcagccgc tcgtcgttct gccgcagatt tccgtcagct
    11101 tcggtactgc cgccggagtc atccctctgg gagaaaagtc ctttcctctg catgtgaccc
    11161 ttacgaatga acagcaggat tctgccgatg gcgaactcca tctggagctt cccgccggat
    11221 ggactacagg gccggcaaca gcgaagtttc atctcccggc aaatgatcct gtcacggtta
    11281 catttactgt gtaccccaca gccttgagca gcgacgcggc ttacaacatc agggcagtcg
    11341 cccgcagcgg caattacgaa ttcagcgaga gcgtgcagca ggtgggttat cccggactgc
    11401 gtccgtatta ctactaccgc ccatccacat atcgcgcgcg cgcagtggat gtgaaggtcg
    11461 cacccgggct gcatgtcggc tacatcatgg gtacgggcga tgaagttcca gaggcgcttg
    11521 aacagatagg tattgatccg catctgctga cgtcagatga aattctgcac ggcgatttaa
    11581 gccagtatga tgcgattgtc ctcggtatcc gcacgtatgc tgcccgtccg gaactggccc
    11641 aggccactgg ccgtttgctg gattacgtaa aaaatggcgg gacagtcata gtccaataca
    11701 acagccccga gtatgaccat aattacgggc cgtatccata cacattgggg cgctctccgg
    11761 agaaggttgt ggatgagaaa tcgcaggtca cgattctcga tcccagccat cctttaatga
    11821 cctggccgaa ccacattacc gaagccgact ttaacggatg ggtagaagag cgcgggcacg
    11881 acttcatgga atcctgggac ccacattaca ccgcgctgac agaaacgcac gatcccgggc
    11941 aggacccgca gaagggtggc ctgctctatg cgcactatgg caaaggtgcc tatatctacg
    12001 tggcctacgc actctaccgg cagatggccg aagccgtccc gggagcatac agaatatttg
    12061 cgaatttagt cagtgctgga aaagaccata tggcccaacg ctccgcccgc ccataggaac
    12121 atgtgtcgtg gcatggataa gctagtctct accagtcaat cttctccggg aaatattccg
    12181 caatcagatc gtcgtagtag ggcttcagtt ctttcaaatt aggtttggtg tggcccttgg
    12241 agtagaggtc gtaagggttg aacttgcgca cccactcaaa catctcttcg tcgtgctcgt
    12301 tcatcaggtg tttgtaggcc ccatgcttgt gcgccggata gaacgagtga tagcgcagca
    12361 tatagagcgc cggttccggc aaataaggtt ttgccacgtg atagatgtat tcgtcgtgtc
    12421 cccatgacaa atggacgttg tccagaccgc agtgcggctc atagatgccg tattccgttt
    12481 ggtattcggg gactttgctg tccgggttcg cgccgaagta ttcgcggaag acaatctcat
    12541 cggcccatgc gcagcccacc ggaaaggtat cgcccaccac ggcccattgc ggttc
//
LOCUS       DASRBV010000397         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_294275, whole genome shotgun sequence.
ACCESSION   DASRBV010000397 DASRBV010000000
VERSION     DASRBV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294275"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..606
                     /locus_tag="VFA02_14395"
     CDS             100..606
                     /locus_tag="VFA02_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZU11092.1"
                     /translation="MGIRSMLDRFTTGDVLDLTGVTARQLQWWDEKGVVVPKREGRNR
                     IYSPEDLVEIIVIEELRRRRISLQQVRRVLRFLRKEVHARLADIVQGREEHHLLLDDR
                     HVYFESDSQQIIDLMRNTKQPMLLICLTDAMRRLKVDLKEILAQAQKKPVSTARAGRR
                     ATETQRTA"
     gene            663..926
                     /locus_tag="VFA02_14400"
     CDS             663..926
                     /locus_tag="VFA02_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11093.1"
                     /translation="MYAELQLLEEWIPEQMEPGTLFVLENAGNMGDARNPYWAVLACP
                     RCGALGLITKAQFIGLESIICGANNCSAEYFLRDDEIEYRKPH"
     gene            complement(1063..1413)
                     /locus_tag="VFA02_14405"
     CDS             complement(1063..1413)
                     /locus_tag="VFA02_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZU11094.1"
                     /translation="MSMKLNTRQVDGVTILDLSGRITLGEGSVQLRDAIRDLLAKGQK
                     QILLNLADVNYIDSSGIGELVSAYTTVRNQGGELKLLNLTKKVHDLLQITKLYTVFDI
                     KDDEASAIASYARA"
     gene            complement(1433..1903)
                     /locus_tag="VFA02_14410"
     CDS             complement(1433..1903)
                     /locus_tag="VFA02_14410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU11095.1"
                     /translation="MSRHYGGEDHLTGSKASRITYTLESSLDSVNRVEQTAEQMAKKA
                     GMEEDEIFRVAMAVREAAVNAVLHGNSYDPEKRITASFENTGNSLVIRIADQGKGLDP
                     ETLPDPLAPENLLRGSGRGIFLIRSFMDEVHFKQLHPGTELTLIKHLGSAHTGS"
     gene            complement(2116..2874)
                     /locus_tag="VFA02_14415"
     CDS             complement(2116..2874)
                     /locus_tag="VFA02_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZU11096.1"
                     /translation="MPATFTRSTTDIERKDTGIGGKPPVDRRPTGGGGGDDNWDHHPN
                     GRRGPRELLSRYRLGLFFALAGDLVFFITLVSAFFVRQNAGHFDVRDNYVSDWHPLAI
                     PPILWINTAVLLLSSLTMEMARRHLFHEIDVMEEWLGLGRPAVRRAAPWLIGTAVLGA
                     LFLTGQWMAWQQLASQGLFFATSQNSHFFYLVTATHGLHLLLGIIALAVALCALFLLR
                     KVELRQIAVDCTAWYWHTMGAFWIFIFVLLTYFQ"
BASE COUNT          696 a          735 c          863 g          637 t
ORIGIN      
        1 tgtgaacata agaaattact gtatgcgcaa aatccaggct tgacagaagg gccggagagg
       61 agaagaatcg catcaaacat ttcttacagg aaatgtttga tgggcatccg atccatgctc
      121 gaccgcttca ccaccggcga cgtccttgat ttgacaggag tgactgcgcg ccagttgcag
      181 tggtgggatg aaaaaggcgt tgtggtcccg aagcgggaag gccgcaaccg catctactcg
      241 ccggaagacc tggtagaaat cattgtcatt gaggagcttc gccgcaggcg catttcgttg
      301 cagcaggtgc ggcgtgttct gcgttttctc cgtaaagaag tgcatgctcg gctggcggac
      361 atcgtgcagg gcagggaaga acatcatctt ctgctggatg accggcatgt ctactttgag
      421 tcggattcgc agcagatcat tgaccttatg cgcaacacaa aacagccgat gctgttgata
      481 tgtttaacgg acgcgatgag gcgtctcaaa gtggatttga aggaaattct ggcccaggcg
      541 cagaagaagc ctgtgagtac agccagagcc ggaaggaggg caacggaaac acaacgaaca
      601 gcgtaatttc caggcaatcc ccacaaacaa attcaggaag ccaaccagaa aaggaggcga
      661 ggatgtacgc agaactgcag ttgcttgagg aatggattcc ggaacagatg gagccgggaa
      721 cattatttgt gctggaaaac gctggaaata tgggcgatgc gcgcaatcct tactgggcgg
      781 tgctggcatg tccgcgttgt ggcgccctcg gcctgattac caaagcgcag ttcatcggtc
      841 tggagtctat tatttgcggt gcaaacaact gttcagcgga atattttctg cgcgatgatg
      901 agatcgagta caggaaacca cattagggtg cgaacaggcc ctgatgactg tcctcttggc
      961 aggatcgcga caaaggccta aaagggctgg tactccattg aatccaggat ttcagaaaaa
     1021 aaggccgcga aaggcggcct ttcctgcata gaaaaaagca ttttaagcgc gtgcatagga
     1081 cgcgatggcg ctggcttcgt cgtccttgat atcgaagacg gtgtaaagtt ttgtgatctg
     1141 cagcaggtcg tggaccttct tggtcagatt caggagcttc agttcgcctc cctggttacg
     1201 gacagtcgta taggcgctca cgagctcgcc gatacccgag ctgtcaatat agttcacgtc
     1261 agccaggttc agcagtatct gcttttgccc tttggccaga aggtcacgga tggcgtcgcg
     1321 caactggacg ctgccttcgc cgagagtgat gcggccgctg agatcgagaa tagttacgcc
     1381 gtcaacctga cgggtgttca acttcatgct cacgaaagat tcctccttaa aactaactgc
     1441 cggtgtgggc gctacccaga tgcttaatga gcgtcagctc ggttccagga tgcaattgct
     1501 tgaagtgtac ctcatccatg aaggagcgga taagaaagat tccccttcct gaacctcgca
     1561 agagattttc gggggcgaga gggtcgggaa gagtctccgg atccaggccc ttgccctgat
     1621 cggcgatgcg aattaccagc gaattacctg tattttcaaa agatgccgta attcttttct
     1681 ccgggtcgta agaattgccg tggaggactg cattcactgc tgcttcccgg acggccatgg
     1741 ccacccggaa aatctcgtct tcctccattc ctgctttctt cgccatctgt tcggcggttt
     1801 gttccacccg gttcacgctg tccagcgagg attcgagtgt gtaggtgatc cggcttgctt
     1861 tcgatcctgt caaatggtct tccccgccat agtgcctgga catccgtgaa attttcctat
     1921 tgagggcagt ttgcttgctt ggaacaacaa tgtcaagcgg agtgatgaaa atgcgtgaaa
     1981 gaaagaggcg ccgggcttcg gcgcctcgct gagaagaatg gttgttagaa caggattttc
     2041 agcgacagtc gaagctggcg cgggaagttc tgcgtaggac cgattgtgcc gtgtccggac
     2101 caggcccccg gctgattact ggaaatacgt cagcagcacg aaaatgaaga tccagaaagc
     2161 gcccatggtg tgccagtacc aggcggtgca gtccacggca atctggcgca gttctacttt
     2221 gcgcagcagg aacagggcgc agagcgcaac cgctaaagca atgatgccca ataacagatg
     2281 gaggccgtgg gttgcagtca cgagatagaa gaagtggctg ttctggctgg tggcgaaaaa
     2341 cagtccctgg gaagcgagct gctgccatgc catccactgg ccagtcagga aaagcgcgcc
     2401 caggacggct gtcccaatga gccacggagc agcgcggcga acagcggggc gtcccaggcc
     2461 gagccattct tccattacgt caatttcatg aaataaatgc cgcctggcca tttccatggt
     2521 cagactgcta aggagcagga cggccgtatt gatccagaga atgggcggaa tcgccagcgg
     2581 atgccagtcg gaaacgtagt tgtcgcgaac atcgaagtgt ccggcattct ggcggacaaa
     2641 gaaggcactc accagagtga taaagaagac cagatcgcct gcgagagcga agaaaaggcc
     2701 gagccggtag cgggagagga gttcacgcgg gccgcgccgt ccgtttgggt ggtggtccca
     2761 attgtcgtcg ccgcctccgc cgcctgtagg ccttcgatct acgggcggtt tgcccccgat
     2821 tccagtgtct ttccgctcga tatcggtcgt ggaacgggtg aaggtagcgg gcatggcgca
     2881 attccctccg ccaacagctt actcctgttt ttgttggatg gaacactgca t
//
LOCUS       DASRBV010000398        11916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_554584, whole genome shotgun sequence.
ACCESSION   DASRBV010000398 DASRBV010000000
VERSION     DASRBV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554584"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3462
                     /locus_tag="VFA02_14420"
     CDS             <1..3462
                     /locus_tag="VFA02_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU11097.1"
                     /translation="LKPGKYSVTVETAGFATEQKTNIELQVGQTATIDFVLKVGAATE
                     TVNVSSEALLNVSKADRGEVIENTRVTELPLNGRDPGMLSILVAGASWTGSPQWQRPF
                     DDTQANLAVNGGQPGNVALLMDGVTNSSTPINNSGQSQISYVAPVDSVQEFKILTNPY
                     DAQYGLMAGAVEDVVLKSGTNTLHGDVYEYARRTWLDANTWSNDYYIGMHRNDPTFDT
                     TPFRTPKMKWDQYGAELDGPVYLPKIYDGRNKSFFTLQYENWHELEPNTITDSVPSPQ
                     WANGDFTNLVYWNGNDQKYEPITLYDPLNIHQDANGTWKRVPFGPSDTINPTPAPNII
                     PASRINPMAQKIIKMYPAPNTTPPTGSNPFANNYVVAGADEDRYRNALGKFDQNLSSK
                     DRMTLIYGYWERVEVRSYDGFTTPGTRGQLPHGERSHTFTLTETHTFTPNLLFDFRAR
                     VGVRADYSYNGPRYDPTNLGWSAQQVAAMGPAALSVFPYLDISEFNSLGTDSNGQSVK
                     NSLSLLPSVTWIKGKHTIHAGLDARFWQIGYNVIGGGNNFWIDRTWTQYNCGSCGSWD
                     PASGNSIASLLLGNPTSGSDTIGVKTYWSSHYWAPFFQDDWKLTRKLTLNLGLRWDFV
                     EPATERHNYSNGGFDATAVNPISSQVTVPGYSQLLGGVTFLGVNGYSRTPYPLSKWDI
                     QPRVGVAYALNDKTVLSAGFGESTRTPQNAPNNIGYSAQTSYQANDPNWPGATHPNLA
                     NQLDNPYTTIIQPTGSSLGMLTALGTGPWTINPKYIIPTFWNYSVGVERQLSRNTVIN
                     LAYVGSRLYNGDCNNNTFRGSCPNINHVSAAAMTNCNPENGGRYENCDNNNVTNPFKG
                     VSAFQGTSYYSSSTINFLNLTRPFPEFTDMTMWQANTAHTWYNSLQLTGTHRMNALTL
                     HGTWTWSKMMDEGGVLDTTYLTPFRQIDGGDITHRITISGVYMLPVGRGRLLLSHTNR
                     ILDTALGGWELGGLYVYQSGNPWLLPGNPSEIYLRSAWVKPHIQKDSGFIRLVAPCVE
                     QWQENNGVYSIVKLQSYDYDGSCAQGSAFRQVPRYAPVPNNIDTGIRHLANQQTDVNL
                     SKNFALYERLNLQLRIEAFNVFNHPLWSMDPDDSTNDSTFGMIQRGPTGQSNLPRQMQ
                     LSAKIRW"
     gene            complement(3533..7027)
                     /locus_tag="VFA02_14425"
     CDS             complement(3533..7027)
                     /locus_tag="VFA02_14425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU11098.1"
                     /translation="MKQIFYDPQRKRWKRLRRVLDVSAVTLTVVLAVFIFSVWKRQSL
                     PELLLPTQKRNYKALKPPELHKTAQRPARRRTHRRASEIPFNADEGLRAAFYVNDEPS
                     YSSLKAHIHQIDMLFPDWLHVTRPDGKLQGATSLFPVHVYDVVDSSGVHGVDEQNKVA
                     RVIAAAKEDTEIFPMLSNYDVIAGTWNGAAIGEMLENPTARESLHVQLDKFLAANPSY
                     RGICLDFEEVPEKDRKLYADWIGELYNDFKAKNLRIYVNVEVSAPDFLLKALAKNNDG
                     IILMNYDQHETSSGPGPIAAENWFESNLTRVLKLVPKEKIICAIGNYGYDWSLPLPEK
                     GKKPSNKVLDAEDLTVQEVWQRAAESDADVHLEGDELNPHFVYDDEDEHVRHQVWFLD
                     GVTVLNELRAARSMGLRTFALWALGKEDGSLWAIWDHPSAKDAPQLLKDVPPGNDVNT
                     EGEGDILRVVSRPQRGSRTISMDADNFTITDEKMTELPRSYTLHYYGYQPKKVALSFD
                     DGPDPVWTPKILDILKQYQVKATFLVIGEEAADHTSLLKRYVREGHEVGNHTFTHPDI
                     SEISQRQLDLELNLTERLFAAELGVQPLYFRPPYSIDQEPDTNDQAAPVDHIQQMGYI
                     ILGNKIDTEDWNEHPRKSPQEIVDTVLQQLEQMKTRPDFRGSVILMHDGGGDRTVTVA
                     ALPLLITTLRAKGYEFVPASELMGKTTAEVMPPIAPNLRWQARIDAVAFLIYGFFSHF
                     VVFVFFAGDVLMSARLVLIGVFALIDRVRKRKIHGPADFAPRVAVLIPAYNEEKVIVR
                     TVRSVLNSTYHNLHVVVIDDGSRDRTFDVVREAYPKEIAEGRLLVLTKPNAGKAEALN
                     FGLQHIEEEFYVGIDADTVIAADAIEKLVVHFADGRVGAVAGNAKVGNKVNLLTRWQA
                     LEYITSQNFERRALDLFNVVTVVPGAIGAWRTAAVIKGGGYPVNTVAEDADLTMNLLE
                     QNYKVIYEDRALAFTEAPTTVNGLMRQRFRWSFGILQAVFKHKHAFRTNRAMGLFALP
                     NIVVFQILLPLVSPFIDIMFAAGALQYWWNKHFHPAAASAASFEKLLVYFLAFLVIDF
                     VTSALAFALERRHPANKGDGWLLFHIWLQRFSYRQIFSLVLFRTLKRAIDGRPFNWDK
                     LERTAKMSQHTEKIAAGG"
     gene            7300..8661
                     /locus_tag="VFA02_14430"
     CDS             7300..8661
                     /locus_tag="VFA02_14430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU11099.1"
                     /translation="MLVVLPRRWWIALFALGAGAVLRLWFIHAYPEIQGDPLVYGDIA
                     KNWMQHGIYGLSWAQGIRPTLIRLPGYPLFLMLCFRLFGMEHYHAVMYAQVAIDLASC
                     LLIAGAAAKMASRRAGWAALWLAALCPFTANYTATPLTETLELFCISLALFSFVRLLE
                     NPQWRWLFLLAFAFSYAALLRPDGALLAVAFCPALVFYGAKLWGTRLMLRMASVCALL
                     SILPFVPWTIRNWHTFRVFQPLAPRYANDPGEPTFPGFNRWTRTVCVDLTCTWEIYWN
                     ANADELHIENLPSRAFDSTAQYLETKQLFDDYNQTTTISSDIDARFARLARERIHDDP
                     VRYYFALPMARLLDMWLRPRTELLWIELRWWEYSKHHAETEFAAAYAALNLAYLLVAC
                     IGFYRWPRFSGAILAFILLRCALLATIEAPEPRYTLECFPMLIALAGVAFERKAMFLR
                     LPV"
     gene            complement(8841..11411)
                     /locus_tag="VFA02_14435"
     CDS             complement(8841..11411)
                     /locus_tag="VFA02_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU11100.1"
                     /translation="METEQKPALGWTTAAKIAWRELHASRTKFIFVILSVAIGVAALT
                     GVRGFSESFQRALLNQARSIMAADLSARMFRIITPQENAKLDALAQAKAIRRTWVTEM
                     VSMASRQGDPVPLLVSLKVVDPAVYPFYGKVVLNPAGDLRSALTDSTVVVDDNLLVRL
                     RTGVGAQLRIGGQNFRIAAVIQKEPDRLTAGVGMGPRVMMTRAAVEKTGLLQAGSRAT
                     ERYLFKMGPQSGRVQDVRAEIEKILPDAQISDFRETSPAITEGLDHATGLLSLICLVA
                     MVLGAIGVAMAMRAHLQQRIDTLAIMKSIGARSQDILRIYLFQTLLLGLAGSVLGIVL
                     GVAVEWALPSLFGQLLPLHPPLRLPLRSVLAALGTGILTTLLFCLPPLLDVRKIRPSV
                     VLRKTVEGDAEKPGWATRLRENKAQWISSIAVLLALGAIAAGLADSALVGKWFTAALA
                     GILIVVLALAAFMLRTLRAFLNKTRLHLHSALRHGLANLYRPGNQSAAVLAALGVGVM
                     LILSVFLMQNAIINRLNSDSTTGAPNIFLIDIGSNELSGVKDLLEHQPGIKGRIETIP
                     IVTARISAVDGVDAAHLNLKNYPRRMLSSVPVTWSDAIPEGVKVLQGKWWQKSDADGL
                     AVVDRVARRLNLHPGSEVDFAAGEKQIRTHVAVIYKPEGEHAFARSEFVLPSGQLQTL
                     PAVWYATVHIQPNQIGAMERALFAAYPTVTVINVADILETVQGVIHQITIVIRFLAAF
                     SILSGAVILASSVASTRFRRIREVVVLKTLGATRNRIASVFSIEFTLLGLLAGLVGTA
                     FANLLTAVLLRKMEVAYHPEVFASAVSVVATAVLAVITGWIASFRILGQKPLEVLREE
                     "
     gene            complement(11401..>11916)
                     /locus_tag="VFA02_14440"
     CDS             complement(11401..>11916)
                     /locus_tag="VFA02_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU11101.1"
                     /translation="DAPTEGEVLIDGTEISRLPEDKLAQVRGKKIGFVFQSYQLIPTL
                     TALENVLLPYELNANGDGKHRAISLLETVGLADRLHHYPVQLSGGEQQRVALARAFVL
                     GPPIVMADEPTGNLDSKNGQHVLDLLTERQREAKTTLVMVTHDKQIADRADRRIMMKD
                     GRIIADETNGN"
BASE COUNT         2630 a         3681 c         3032 g         2573 t
ORIGIN      
        1 ctcaaaccag gaaaatattc cgtcacagtc gaaactgctg gttttgctac cgagcagaag
       61 accaacatcg agctccaggt ggggcagacc gcaaccattg actttgtgct gaaggtcggc
      121 gcggccactg aaacggtgaa tgtttcgtca gaggcgctcc tgaacgtcag caaggctgac
      181 cgcggcgagg tcatcgaaaa tacccgagtc actgaacttc ccctgaacgg acgcgatccg
      241 gggatgttat ctatcctggt cgcgggcgcc tcctggaccg gcagcccgca atggcagcgt
      301 ccttttgacg acacgcaggc caacctcgcc gtcaacggag gccagccggg aaacgtcgcc
      361 cttctgatgg acggagtcac caactcctcg accccaatta acaattccgg ccagtctcag
      421 atttcgtatg tcgctcccgt ggactccgtg caggagttta aaatcctcac caatccttac
      481 gacgcccagt atggacttat ggcaggggcc gttgaggacg tcgtcctcaa gtccgggacc
      541 aatacacttc atggcgatgt gtatgaatat gcacgccgca cctggctcga cgccaatacc
      601 tggtcaaacg attactacat cgggatgcac agaaacgacc ccacctttga cacaacgccg
      661 ttccgcaccc caaagatgaa gtgggaccag tatggagctg aactggatgg ccccgtttac
      721 ctgccaaaaa tctacgatgg caggaacaag tcatttttta ctttgcaata tgagaactgg
      781 cacgagctgg aaccgaacac aatcacggat tccgtaccca gcccgcaatg ggccaatgga
      841 gacttcacga acctcgttta ttggaacgga aacgaccaga agtacgaacc catcacgctc
      901 tatgacccgc tgaacattca tcaggacgcc aacggcacat ggaagagagt gcctttcggc
      961 ccttcagata cgatcaatcc cacgcctgcg ccaaacatca ttcctgcttc gcgcatcaat
     1021 cctatggcgc agaagatcat caaaatgtac cccgcgccaa acaccacgcc tcctacaggg
     1081 agcaatccat ttgcaaacaa ctacgtggtt gctggggccg atgaagaccg ttaccgtaac
     1141 gccctcggca agtttgacca gaacctttct tcaaaagatc gcatgaccct catctatggc
     1201 tactgggagc gcgtagaagt ccggagttat gacggcttta ccaccccagg cacccgcggt
     1261 cagttaccac acggcgaacg cagtcatacc tttacgctga ctgagaccca cacttttaca
     1321 cccaatcttc tcttcgattt ccgggcgcgc gttggtgtcc gcgcagacta cagctacaat
     1381 ggtccgcgct atgacccgac aaatctcggc tggtcggccc agcaggtggc cgcgatggga
     1441 cctgctgcct tatctgtgtt tccatatctc gacatcagtg aatttaactc tcttggaaca
     1501 gacagtaacg gccaaagtgt gaaaaattcg ctttctctgc ttcccagtgt cacctggatc
     1561 aaaggcaaac acaccatcca cgcgggtctg gacgcccgct tctggcagat tggttacaac
     1621 gtcattggcg gcggcaacaa cttctggatt gaccgcacct ggacacagta caactgcggg
     1681 agctgcggct cctgggaccc ggccagcggc aactccattg cttctctgct gctcggcaat
     1741 cccacttccg gctccgacac catcggcgtc aaaacctact ggagctccca ttactgggcg
     1801 cccttcttcc aggacgactg gaagctcacc cgcaagctca cgctgaacct cggcctgcgc
     1861 tgggacttcg tcgaacccgc aaccgagcgc cataactatt ccaatggcgg ctttgatgca
     1921 accgccgtta accccatcag ctctcaggtg accgtacccg gttacagtca gctcctcggc
     1981 ggcgtgacct tcctgggagt gaatggctac agccggacac cttatccgct ttcaaagtgg
     2041 gacattcagc cccgcgttgg cgttgcttat gctctgaacg acaagaccgt cctcagcgca
     2101 ggctttggcg agtccacccg cacaccgcaa aacgccccga ataatatcgg ctatagcgcc
     2161 cagacctcct accaggccaa cgatccgaac tggcccggtg caacccaccc caatttggca
     2221 aaccagcttg ataatcccta caccaccatc attcagccta caggttccag tctggggatg
     2281 ctgaccgcgc ttgggaccgg accctggacc atcaacccca aatacatcat tcccaccttc
     2341 tggaactact cggtcggcgt agaacggcag ctttcgagaa ataccgtcat caacctcgcc
     2401 tatgtcggca gccgcctcta taacggagac tgcaataaca ataccttccg tggctcctgc
     2461 ccgaacatta accacgtcag cgcggccgcg atgaccaact gcaaccccga aaatggcgga
     2521 cgctacgaaa actgtgacaa caacaacgtc acaaatccct tcaaaggcgt aagcgccttt
     2581 cagggaacca gctattattc gtcatccacc attaatttcc tcaacctgac ccgcccattt
     2641 cctgaattta ctgacatgac catgtggcag gccaataccg cccatacgtg gtacaactcc
     2701 cttcaactaa cgggcactca caggatgaat gcgctcacgt tgcatgggac ctggacctgg
     2761 tcaaagatga tggacgaagg cggcgtgctg gatacgactt atctcactcc gttccgccag
     2821 attgatggcg gcgacatcac ccaccgcatc accatttccg gcgtctacat gctgcctgta
     2881 ggacgcggac gcctgctcct ctctcatacc aaccgcattc tggacaccgc gcttggcgga
     2941 tgggagttgg gcggcctcta cgtctatcag tcgggcaatc cctggctatt gcccggaaac
     3001 cccagtgaaa tctatctgcg cagcgcatgg gtcaagccgc atattcagaa agacagcgga
     3061 tttattcgcc tcgtggcccc ctgtgtggag cagtggcagg aaaacaacgg tgtctattcg
     3121 attgtcaaac tacaatcgta tgactacgac ggaagctgcg cacaaggctc ggccttccgc
     3181 caggtcccgc gctatgctcc agtccccaac aatattgaca ccggcatccg tcatcttgcc
     3241 aaccagcaga cagacgtgaa cctgtcgaaa aactttgcgc tctatgaacg cctgaacctc
     3301 cagttgcgca ttgaggcgtt caatgtcttc aatcacccgc tgtggtccat ggacccggac
     3361 gacagcacca atgactccac ctttggcatg attcagcgtg gcccgacagg acagagcaat
     3421 ctgccccgcc agatgcagct atcggccaag atccggtggt aagacaggca cagcatcccc
     3481 ttaatcccca aagagaccgc tttcgagcgg tctctttgtg tctgcgaaaa gatcatcctc
     3541 cggcagcaat cttttccgtg tgctgtgaca tctttgccgt gcgctccagc ttgtcccagt
     3601 tgaagggacg cccatctatg gcgcgcttca gcgtcctgaa caacaccagc gagaaaatct
     3661 gtcggtacga aaatcgctgt agccagatgt ggaacagcag ccatccatcg cccttgttcg
     3721 ccggatggcg gcgttccagc gcaaaggcca gcgccgaggt gacaaagtca atcaccagaa
     3781 acgcgaggaa gtaaaccagc agcttctcaa agctcgccgc gctggccgcc gcaggatgaa
     3841 agtgcttgtt ccaccagtac tgcagcgcgc ctgccgcaaa catgatgtca ataaagggcg
     3901 agaccagcgg cagcaaaatc tggaaaacaa caatatttgg cagtgcaaac aggcccatcg
     3961 cgcgattcgt gcggaaggcg tgcttgtgct tgaagacagc ctgcaggatc ccgaaagacc
     4021 agcggaagcg ctgccgcatc agaccattga ccgtcgtggg cgcttcagta aatgccagcg
     4081 cgcggtcttc ataaatgacc ttgtaattct gctccagcag gttcatcgtc aggtccgcat
     4141 cttccgctac cgtattgacg ggatatcctc cacccttgat caccgctgct gtgcgccaag
     4201 ccccaattgc acctggaacg actgtgacta cgttgaacag gtccagcgcc cggcgctcaa
     4261 agttctggct ggtgatgtac tcgagcgcct gccagcgcgt aagcaggttc accttgttgc
     4321 cgacctttgc gtttccggca accgcgccca ctcgcccgtc cgcaaaatgc acaaccagct
     4381 tttctatcgc gtcagcggca atgaccgtat cagcatcaat accgacgtaa aattcttcct
     4441 caatgtgctg caggccaaaa ttcagtgcct cagccttgcc cgcatttggc ttcgtcagga
     4501 ccagcagcct gccctcagca atctccttcg gataagcctc acgaacaacg tcaaacgtcc
     4561 ggtctctcga cccgtcatca atcacaacaa cgtgcaggtt gtgatacgtc gagttcagca
     4621 ccgagcgcac tgtgcggacg atgaccttct cctcgttata agcaggaatc agcacagcca
     4681 cacgtggcgc gaagtctgca gggccatgaa ttttgcgctt acgcacgcgg tcaatcagcg
     4741 caaagacgcc aatcagcacg agacgcgcgc tcatcagcac gtcgcccgca aaaaacacaa
     4801 agacgacgaa gtggctgaag aacccataaa tcagaaacgc aacggcatca atccgcgcct
     4861 gccagcgaag gttcggagca atcggcggca tcacctccgc cgtggtcttg cccatcagct
     4921 ctgaagcagg aacaaactcg tatcccttcg cgcgtaatgt ggttatcagc aaaggcagcg
     4981 ccgctactgt cactgtgcgg tccccgccgc catcgtgcat cagaatcacg ctgccgcgaa
     5041 aatcgggtct ggtcttcatc tgctccaatt gctggagcac tgtgtctaca atttcctgcg
     5101 gcgacttgcg tggatgctca ttccagtcct cggtatcaat cttgttaccg agaatgatgt
     5161 agcccatctg ctggatgtgg tctacaggcg cggcctgatc gttcgtgtcc ggctcctggt
     5221 caatcgagta tggaggccgg aagtaaagcg gctgcacacc aagctcggca gcgaacaacc
     5281 gctccgtcag gttcagttcc agatcaagct gccgctgcga aatttcactg atgtcaggat
     5341 gcgtaaaggt atgattgcca acctcgtgcc cttcgcgcac atagcgtttc agcaggctgg
     5401 tatggtctgc tgcctcttct ccgatgacca gaaacgttgc cttgacctga tactgtttca
     5461 gaatatcgag tatcttcggt gtccataccg ggtccggacc atcgtcaaat gacagcgcga
     5521 ccttctttgg ctgatagccg taatagtgca gcgtgtaaga acgcggaagc tccgtcatct
     5581 tctcatcggt gatggtaaag ttgtccgcat ccatgctgat ggtgcgcgaa ccacgctgcg
     5641 ggcgcgacac aacacgcaga atgtcgccct cgccctccgt gttgacgtca ttgcccggtg
     5701 gaacatcctt cagaagctga ggcgcgtctt ttgcgctggg atggtcccag atggcccaca
     5761 gcgagccgtc ctcttttccc agtgcccaga gcgcgaaggt cctcaggccc atgctccgag
     5821 cagcacgaag ctcattcaaa acagtcacgc catccagaaa ccacacctga tgacgcacat
     5881 gctcatcttc atcgtcgtag acaaagtgcg gattcagctc gtcgccttcc agatgcacgt
     5941 cggcatccga ttctgccgcc cgctgccaca cctcctgcac tgtcaggtct tcggcatcca
     6001 ggaccttgtt gctgggcttt ttgcctttct ccggcaaagg aagagaccag tcatatccat
     6061 agttcccgat ggcgcagatg atcttctctt tgggcaccag cttcagcacg cgagtcagat
     6121 tgctctcaaa ccagttctcc gccgcaatcg ggccaggccc gcttgaagtc tcatgctggt
     6181 catagttcat caggatgatg ccatcgttgt tcttcgccag cgccttcagc aggaaatcag
     6241 gcgcgcttac ctccacgttg acgtagatgc gcaggttttt cgctttgaaa tcgttataaa
     6301 gttcaccgat ccaatcggcg tagagcttac ggtccttttc cggtacctct tcaaaatcaa
     6361 ggcatatacc gcgataactg gggttggctg cgagaaactt atcaagctgg acatgcaggc
     6421 tctcgcgtgc cgtggggttt tccagcattt caccgatagc agcaccgttc cacgtgccgg
     6481 caatcacatc gtaattgctc agcatcggga agatttccgt gtcttctttt gctgcggcaa
     6541 tcacacgcgc cactttgttc tgctcatcta ctccgtggac gccggaagag tccaccacgt
     6601 cgtaaacatg cacaggaaag agcgatgtcg ccccctgaag ttttccatct ggcctcgtaa
     6661 cgtgcagcca gtcgggaaag agcatatcaa tctggtggat gtgcgccttg agcgacgaat
     6721 agctgggctc atcattcaca tagaaagctg cccgcagacc ttcatccgca ttgaaaggaa
     6781 tctcggaagc gcgccggtgt gtcctgcgcc gcgcaggacg ctgtgcagtc ttgtgcaact
     6841 ctggcggctt cagcgccttg taattgcgct tctgtgttgg cagcagcagc tcaggtaacg
     6901 actgccgctt ccacacactg aaaataaaca cggccagcac taccgtcaat gtaacggcgg
     6961 agacgtctag tacgcgcctc aggcgtttcc agcgcttccg ctgtggatca tagaagattt
     7021 gtttcattca aatccggttg ttcttcttcc ttctacctat tgtatgtgtg ctcgccattg
     7081 ctgattccaa gtacttagtt gtaacttctt gtaaacggct tcaggcgcag gcccgcgcca
     7141 tcggcttgga ccggaaagcc gcatctttcg ggcgaagcct tccattctgc tgcgcccttc
     7201 acaccagcac gaccctctgg gttccgctga ttcaggaggt gctgaaaaga ttagatctac
     7261 agccctaaaa ttcctaccta ccctgtatga ttcatttgca tgctcgttgt gcttcctcgc
     7321 aggtggtgga tagctctctt cgcgcttggc gccggggccg ttctccggct ctggttcatt
     7381 catgcctatc ccgaaatcca gggagaccct ctggtttacg gcgacatcgc caaaaactgg
     7441 atgcagcatg gcatctacgg cttgtcgtgg gcacagggca ttcgtccaac actgatccgc
     7501 ctgcccggat atccactctt cctgatgctc tgcttccgtc tctttggaat ggagcattac
     7561 cacgccgtta tgtatgccca ggttgcgatt gacctcgcca gttgtctgct gattgcaggg
     7621 gccgctgcga aaatggcttc gcgtcgtgcc ggatgggccg ccctctggct ggctgcactc
     7681 tgccctttta cggcaaacta caccgccact ccactcactg agacccttga gctgttctgt
     7741 atttccctgg cgctgttcag ctttgtgcgg ctgctcgaaa atcctcagtg gcgatggctt
     7801 ttcctgctgg cctttgcctt cagttatgcc gcgttgcttc gccccgacgg agcactcctc
     7861 gcggtcgctt tctgtcccgc ccttgtcttt tatggagcaa agctttgggg tacccggctg
     7921 atgctgcgca tggcgtccgt ctgcgcattg ctctccatct tgccatttgt tccctggacc
     7981 atccgcaact ggcatacttt tcgcgttttt cagccgctcg ctccgcgata cgcaaatgac
     8041 cccggcgaac ccaccttccc aggcttcaat cgctggacca ggaccgtctg cgtagacctt
     8101 acctgcacct gggaaattta ctggaacgcc aatgcagacg agctgcatat cgaaaatctt
     8161 ccctcacgcg cttttgactc gaccgcgcag tacctggaaa caaagcagct ctttgacgat
     8221 tacaaccaga ccacaaccat cagttcagac atagatgccc gctttgcccg gcttgccaga
     8281 gaacgcatcc atgatgatcc ggttcggtat tacttcgccc ttcccatggc gcggctgctc
     8341 gatatgtggc tccgcccacg cacggaactg ctctggatcg agttgcgctg gtgggagtac
     8401 agcaaacatc acgcagaaac cgaatttgcc gccgcttatg ccgcgctgaa ccttgcctat
     8461 cttcttgtgg cctgcatcgg tttttaccgg tggcccagat tttccggggc catacttgcc
     8521 ttcatcctgc tgcgctgcgc cctgctcgcc accattgaag cccctgaacc caggtatacg
     8581 ctcgaatgtt tccccatgct gattgcgctc gcaggcgtgg ccttcgagcg caaggcaatg
     8641 ttcctgcggc tccctgtctg aaggttcatt ccctgcaata acacacactt ttcttacagg
     8701 gaggtgtgcc ctctgcgatc cttatggcct ttttcgtata caaagtccgt cattctgacg
     8761 accgacctat aaactccgtc attctgacga ccaaagggag gaagaatcta tgcattggcg
     8821 gcccaggaac ggctctctcc ctactcctcc cgcaggactt ccagcggctt ctggcccagg
     8881 atgcgaaagc tggcaatcca tccggttatc acggcgagca cggcagttgc gaccacagaa
     8941 acagcgctgg cgaaaacttc cggatggtag gccacttcca ttttgcgcag cagaactgct
     9001 gtcagaagat tggcaaacgc tgtgccgaca agaccggcca gcagtcccag cagagtaaat
     9061 tcgatgctga agacgctggc aatgcggttg cgggtcgcgc ccagcgtctt aagaacgacg
     9121 acttctcgaa tgcgccggaa gcgagtgctg gctacactcg atgccagaat caccgctccg
     9181 gaaagaatcg aaaatgcggc cagaaagcgg atcacgattg tgatctggtg aatgacgccc
     9241 tgcaccgttt ccagaatgtc cgcgacatta atgaccgtca ccgtgggata tgcagcaaac
     9301 aaggcccgct ccatcgcgcc aatctggttc ggctggatat gcactgtggc gtaccagacc
     9361 gccggtagcg tttgcaactg tccggatggc aacacaaact cgctgcgtgc aaaggcgtgt
     9421 tcgccttcgg gcttgtagat gacagcaacg tgcgtgcgta tctgcttttc accagctgcg
     9481 aagtccacct ccgatcctgg atgcagattc aaccttcgtg ccacgcggtc tacaacagca
     9541 agcccatcgg catcgctctt ttgccaccat ttgccctgaa ggaccttcac tccttccggg
     9601 atggcatctg accatgtaac tggcactgac gatagcatcc ggcgcggata gtttttcaga
     9661 ttcaggtgcg ccgcatctac tccgtctacg gcagagatgc gggctgtcac gataggaatc
     9721 gtttcaatcc tgcctttgat accaggctgg tgctcaagca ggtctttcac acctgaaagc
     9781 tcattcgacc caatgtcaat cagaaaaatg ttcggagcgc cggtggtcga atctgaattc
     9841 aggcggttga tgatggcgtt ctgcatgaga aagacgctca gaatcagcat cacacctacg
     9901 ccgagcgcgg ccagcaccgc tgccgactgg ttccccggac ggtacaggtt tgccagtcca
     9961 tgccgcagag ccgaatgcag atgcagccgc gttttattca gaaatgcccg cagggtccga
    10021 agcatgaatg ctgccagcgc caacaccaca atcagaatgc cggccagcgc cgccgtgaac
    10081 cacttgccaa cgagtgcgga atcggccagc cctgcggcga ttgccccgag tgcaagtaat
    10141 acggcaatag aagaaatcca ctgtgctttg ttttcgcgca gccgcgtggc ccaccccggt
    10201 ttctctgcat cgccttccac agtcttgcgc agaacaacgc tgggcctgat cttccgcaca
    10261 tccagcagtg gaggcaggca gaacagcagc gtcgtcagaa tgccagtgcc aagcgcagcc
    10321 agcaccgaac gcaacggcag ccgcagtggt ggatgcagcg gcagcaactg cccgaacaac
    10381 gaaggcaacg cccattcgac cgccacgccc agcacgattc cgagcacaga gccggcaaga
    10441 ccaagcagca gcgtctgaaa gagatagatg cgcagaatgt cctgcgagcg cgcaccgata
    10501 gacttcataa tggcaagcgt gtctatgcgc tgctgcaaat gcgcgcgcat cgccatggcc
    10561 acgccgatgg ccccgagcac catcgcaacc aggcagatca ggctcaacag cccggtggcg
    10621 tggtccagcc cttcagtaat ggcggggctg gtctcgcgga agtcggatat ctgcgcatca
    10681 ggcaggatct tttcaatctc tgcgcgaacg tcctggactc tcccactctg cggccccatc
    10741 ttgaacagat aacgctccgt ggcgcggctt ccggcctgca gcaacccggt cttctctacg
    10801 gcggcgcgtg tcatcattac gcgcggcccc atgcccacgc ccgcagtcag acggtccggc
    10861 tctttctgaa tcacagcggc aatccggaaa ttctgcccgc caatccgtag ctgggcacca
    10921 acacctgtac gcaggcgtac cagaaggttg tcgtccacta cgacggttga gtctgtcaaa
    10981 gcagaccgca aatctccggc aggattcagc acgaccttcc cataaaacgg atacaccgcc
    11041 gggtccacaa ctttgagcga gaccagcaaa ggcaccgggt caccctgccg tgaggccatc
    11101 gagaccatct ccgtcaccca ggtacgccga atggcctttg cctgggcaag tgcatcgagt
    11161 tttgcgttct cctgcggggt aatgatgcgg aacatacgcg cggaaaggtc tgccgccata
    11221 atgctgcgcg cctggttgag caatgctctc tgaaatgatt cgctgaagcc gcgcacgccc
    11281 gtaagcgcag ccacgccaat ggcgacagaa agaatgacga agatgaattt tgtccgcgag
    11341 gcatgcagtt cacgccaggc aatctttgcg gcggtcgtcc agccgagggc aggtttctgc
    11401 tcagtttcca ttggtttcat ccgcgataat gcgcccgtcc ttcatcatga tgcggcggtc
    11461 ggcccggtcg gcaatctgct tatcgtgtgt caccattacc agggtggttt tagcttcacg
    11521 ctggcgctcg gtgagaagat cgagcacgtg ctgtccgttc tttgagtcga gatttccggt
    11581 cggctcatcg gccattacaa tcggcggccc aagaacaaag gcgcgggcga gcgcaacacg
    11641 ctgctgctct ccgccggata gctggactgg ataatgatgc agccggtctg caaggcctac
    11701 ggtctcaagc aatgagatgg cgcgatgctt gccatcgcca ttggcgttga gttcataggg
    11761 cagcagaaca ttttcaagcg ccgtcagcgt aggaatcagt tggtacgact gaaagacaaa
    11821 accgatcttc tttccgcgca cctgtgcgag tttgtcctcc ggtaaacggc tgatttccgt
    11881 gccatcaatc aacacttcgc cttccgtggg agcatc
//
LOCUS       DASRBV010000399         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_346656, whole genome shotgun sequence.
ACCESSION   DASRBV010000399 DASRBV010000000
VERSION     DASRBV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346656"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="ispH"
                     /locus_tag="VFA02_14445"
     CDS             <1..667
                     /gene="ispH"
                     /locus_tag="VFA02_14445"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZU11102.1"
                     /translation="TKVHVEAVKFAKQGYSLILVGHRDHDEIEGTFGEAPDVTQIVSS
                     VEEVEALQVPDPNRVAYLTQTTLSLDEARDIIHALKAKFPNIVGPHSQDICYATENRQ
                     TAVKNVAHNADLVLVVGSNNSSNSNRLVEVSRNLGTNGYLIDNSAAIHPEWLEGVSTV
                     AVTAGASAPEILVEDVVNYLRQKGFNSVEEVEVMPENVRFGLPPEIVQAIAAAPAPAQ
                     ASV"
     gene            741..2747
                     /gene="shc"
                     /locus_tag="VFA02_14450"
     CDS             741..2747
                     /gene="shc"
                     /locus_tag="VFA02_14450"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZU11103.1"
                     /translation="MSTVTGSAQPGIPRFGRIDAALDAVQAAIARSRDFIFSIQHHEG
                     FWCGELEADSMLEADYIFMHRFLGTGDEARLKRALTEMLRFQNEDGSWSIYPGGPGNI
                     SLSVKCYFACKLMGMTQDEPILVKAREWILAHGGVVQCNTFTKIYLCALGQYDYDAVP
                     AVPPEIVLFPRWFYFNIYEISSWSRAILVPLSIAYAKKPFKKIPPEQGIDELFAGGRE
                     NADLRLQWDSRNKISWRNFFLLLDRMMHWAERVHIRPLRKIALKKAEKWMLERFEMSD
                     GLGAIYPAMLNAIIALRCLGYSLDDPQVIRAMDEFEKLGIDAPGGTPDYPEPTFRMQP
                     CMSPVWDTAQAVFALGEAGVPRSDARMLKAADWLLSKEVRHKGDWAMKVHADPGGWYF
                     EFNNEFYPDVDDTAQVLLALNKVDNPRERYQYDVCKRAIDWIFAMQCKNGGWASFDKD
                     NTKMIFQYIPFADHNAMLDPPTVDITGRILEMLAAYGYTRKDKRIEKAIKFIYSEQEP
                     DGSWFGRWGVNYLYGTFLVLRGLEAIGVWNHEPQIQQAAEWVRSVQNADGGWGESCGS
                     YDDPTSRGVGISTPSQTAWAILGLLAAGDTRSDSVAKGVRWLLEHQQEDGGWDESIGV
                     GRQRQAIYTGTGFPRVFYLAYHQYRNYFPLLALTTYKRAMEREE"
     gene            2790..>3958
                     /gene="hpnH"
                     /locus_tag="VFA02_14455"
     CDS             2790..>3958
                     /gene="hpnH"
                     /locus_tag="VFA02_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZU11104.1"
                     /translation="MAVPISQAWTVATYVLKQKLRGRKRFPLVTMLEPLFRCNLACAG
                     CGKIQYPAHILKQQLTPEECFRAVEESGTPMVSIPGGEPLLHPEIDKIVEGLVARKKY
                     VYMCTNALLLKEKLHLFRPSKYLSFSVHVDGQREHHDFSVCREGGYDAAIEGIKAAVA
                     AGFRVTTNTTLFDGADPNSVRAFFDEMMALGVEGMMVAPGYTYEKAPDQKHFLGRARS
                     RRLFRAILSNRKKAWKFNASPMYMEFLMGRREFSCTPWGMPAYSVFGWQKPCYLLQDG
                     YADTFQELIETTDWSSYGTESGNPKCANCMVHSGYEASGVYYTFGSLRGFLDTAIATL
                     FDKYEDKEALKLLNEPVRPVHSYNPLVQIETKINVVETHAAHNPDELEVAEGRER"
BASE COUNT          876 a          998 c         1210 g          874 t
ORIGIN      
        1 gaccaaggtc catgtggaag cagtgaagtt tgccaagcag ggctattcgc tgattctggt
       61 gggtcatcgc gaccacgatg aaatcgaggg tacgtttggt gaagctccgg atgtaacgca
      121 gattgtttca agtgtggaag aggtagaggc attacaggtg ccggacccca accgcgttgc
      181 gtatctgacg cagaccactc tgtcactgga tgaagcgcgg gacatcattc atgcgctgaa
      241 ggcgaagttt cccaatatcg ttggtccgca ttcgcaggac atttgctacg caacggaaaa
      301 ccgccagacg gctgtgaaga atgtggcgca caatgccgat ctggtgctgg ttgttggttc
      361 gaataacagt tccaactcga accgtcttgt ggaggtttcg cgcaatctcg ggaccaatgg
      421 ttatctgata gataattctg cggcaatcca tcctgaatgg ctggaaggcg tttctacggt
      481 tgcggtcacc gccggtgctt cggcccctga gattctggtg gaagatgtgg taaattactt
      541 gcgccaaaag ggatttaaca gcgtcgaaga agtggaagtg atgccagaga atgtacgttt
      601 tggccttcca ccggagattg tgcaggcaat tgctgcggct ccggcgcctg cacaggcatc
      661 tgtataaaaa gcaagcaaat ttgaatccga tctcatacag agatttgagg ttttaacgag
      721 ggcagttctt tagcgacagc atgagtacag tcactggatc ggcacaaccc ggcattccac
      781 ggttcggcag aattgatgct gccctggatg ccgtccaggc tgccatcgcc cgttcccgcg
      841 atttcatctt ttccattcag catcatgagg gcttctggtg cggtgagctg gaagccgatt
      901 ccatgctgga agccgattac atcttcatgc accgttttct gggtacagga gatgaagcgc
      961 gtttgaagcg cgcgcttacg gagatgctgc ggttccagaa tgaggatgga agctggagca
     1021 tctatcctgg cggcccggga aacatcagcc tttccgtcaa gtgctatttt gcctgcaagc
     1081 tgatgggcat gacgcaggac gagccgatac tggtcaaggc gcgggaatgg attctggccc
     1141 atggcggcgt ggtgcagtgc aataccttta ccaagattta tctctgcgcg ctgggacagt
     1201 atgactacga tgctgtgcct gctgttccgc cggagatcgt gctttttccg cgctggtttt
     1261 atttcaatat ctacgagatt tcttcgtggt cgcgcgcgat cctggtaccg ctttcgatcg
     1321 catatgcgaa gaagccattc aagaagatcc caccggagca ggggattgat gagctttttg
     1381 caggcggcag agagaatgcc gaccttcggc tccagtggga cagcaggaac aaaatcagtt
     1441 ggcgcaactt tttcctgctt ctggacagaa tgatgcattg ggccgagcgc gtccacattc
     1501 ggccattgcg caagatcgcg ctgaagaagg cagagaagtg gatgctggag cgctttgaga
     1561 tgtccgatgg gctgggcgcg atttatccgg cgatgctgaa tgcgattatc gcgctgcgct
     1621 gtctgggata ttcgctggat gacccgcagg tcatccgcgc gatggatgaa tttgagaagc
     1681 tcggaattga tgcgcctgga ggcactccgg attatccgga gcctacattc cggatgcagc
     1741 cctgtatgtc tccggtatgg gacacggcgc aggcggtctt tgcgctgggt gaagcgggtg
     1801 ttccgcgttc ggatgcgcgc atgttaaagg cggcggactg gttgctatcc aaagaagtac
     1861 ggcacaaagg tgactgggcc atgaaagtac atgcagaccc gggcggctgg tacttcgagt
     1921 tcaacaatga attttatccg gacgttgacg acacggccca ggtgttgctg gcgcttaaca
     1981 aggtggacaa tccacgcgag cgctaccagt atgacgtgtg caagcgcgcc attgactgga
     2041 tcttcgcgat gcagtgtaag aacggcgggt gggccagctt cgataaagac aacacgaaga
     2101 tgatcttcca gtacatcccg ttcgctgatc ataacgcgat gctggatccg ccgacggtgg
     2161 acattacggg gcgcattctg gagatgctgg ccgcttacgg ttacacgcga aaagacaagc
     2221 gcatcgaaaa ggccatcaaa tttatataca gcgagcagga gccggacggg agctggtttg
     2281 gccgctgggg cgtgaactat ctttacggca cgtttctggt gctccgcggg ctggaagcaa
     2341 tcggggtatg gaaccacgag ccacagattc agcaggcggc ggagtgggtc cgctcggtgc
     2401 agaatgcgga tggcggatgg ggcgagagtt gcggcagcta cgatgatcca acttcgcgcg
     2461 gcgtcggcat cagcacgccg tcgcagactg cctgggcgat tctgggactg ctcgctgctg
     2521 gtgatacgcg cagcgacagc gtagccaagg gcgtccgctg gctgcttgaa catcagcagg
     2581 aagatggcgg ttgggacgag agtataggcg ttggccgcca gcgccaggcg atttataccg
     2641 gaactgggtt cccacgggtg ttttatctgg cgtatcacca gtaccggaat tacttcccgc
     2701 ttctggcgct cacgacgtac aaacgcgcaa tggaacgcga agagtaaaag accgaggatg
     2761 acagcagagg tttagaacag gggaggaaca tggctgtacc catatctcag gcgtggacgg
     2821 tagccactta cgtcctgaag cagaagctca gggggcgcaa gcgatttccg ctggtcacga
     2881 tgctggagcc gctcttccgc tgcaatctgg cgtgcgccgg atgcggaaag atccagtacc
     2941 ctgcgcatat tctgaagcag caactgacgc cggaagagtg cttccgcgca gtagaagaat
     3001 cgggcacgcc catggtctcc attccaggcg gcgaacccct gctgcaccct gaaattgaca
     3061 agattgttga aggactggtt gcgcgcaaga agtacgtata tatgtgcacg aacgcgctgc
     3121 ttcttaaaga aaaacttcat cttttcaggc caagcaagta tctgtcgttt tccgttcatg
     3181 tagacgggca gcgcgagcac cacgatttct ctgtctgccg cgaaggcggc tatgatgcgg
     3241 cgatcgaagg catcaaggcg gctgtggctg cgggcttccg ggtaaccacg aacaccacgc
     3301 tcttcgatgg cgcagacccc aattccgtcc gcgccttctt tgacgagatg atggcgctgg
     3361 gcgtggaagg catgatggtt gcgcctggct acacctatga gaaggccccg gaccagaagc
     3421 actttctggg acgtgcccgt tcgcgccgcc tcttccgcgc cattctttcc aatcgcaaga
     3481 aggcgtggaa gttcaacgct tcgccgatgt atatggagtt cctgatggga cggcgcgaat
     3541 tctcgtgcac gccatgggga atgcctgctt acagcgtctt cggctggcag aagccctgct
     3601 atcttctgca ggacggctat gccgatacct tccaggaatt gattgagacg accgactggt
     3661 caagttatgg gaccgagtcg ggcaatccca agtgcgccaa ctgcatggtg cattcgggat
     3721 atgaagcatc gggcgtctat tacactttcg gctcactgcg cggttttctg gacacggcca
     3781 ttgccacgct ctttgacaaa tacgaagaca aagaggcgct gaagctgttg aatgagcctg
     3841 ttcgtccggt gcatagctac aatccgctgg tccagattga aacgaagatc aatgtcgttg
     3901 agacgcatgc tgctcataac ccggacgaac tggaagtggc agaagggcgt gagcgcga
//
LOCUS       DASRBV010000400         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_649299, whole genome shotgun sequence.
ACCESSION   DASRBV010000400 DASRBV010000000
VERSION     DASRBV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649299"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFA02_14460"
     CDS             <1..468
                     /locus_tag="VFA02_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11105.1"
                     /translation="AQMEQALGQLQQQFEQMPKYLPSVPVAQDESEDHATEAATLFSW
                     MQGPEGRSLRKAASREPEGGPNWKKWTNCYLHWQGHMQMKAKLAAQNAPQMPPKMTIS
                     IPVDKMHGDAQAQLLQKAGIQVSGPNPAPHEVEEEARIYTPTSEIVRKQKRRL"
     gene            465..1085
                     /locus_tag="VFA02_14465"
     CDS             465..1085
                     /locus_tag="VFA02_14465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZU11106.1"
                     /translation="MKEHLVGYILRHGETELNRNNCFRSWLDVPLDENGLRQAKQAAE
                     YLKDKPLKHVISSPLLRAFVTADMAAAPHRLQVYQHRGLFPWRLGIFSGLEKEENQDA
                     LRLFVKNPAVTIPGGESLLDFEARQFAFWEAALKLARVQGLTLFVAHTSNVVALQNFT
                     QDGEDLEPEDADTVKPGGIAAIYWNGKEHRVEPVFGQAEEAVFGGS"
     gene            1131..2408
                     /locus_tag="VFA02_14470"
     CDS             1131..2408
                     /locus_tag="VFA02_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11107.1"
                     /translation="MADAVIDLAALGDDTGTGSATVATDHSAANETQEASQGAEQDGS
                     QQESQQQSDAEKLDGRRGPQNIRNSIKAAAEALPDHAQAFKELGNSYFREQAYKQVFP
                     TVQEATTAKNLIDSVGGLDGISQLQERDQLYTAQDDMLREGNPEVLDDFFEDFPEGAA
                     ALAPHYLDRLAKMNPEAFSSAVVPYVVGMLENAGIGNYLASILQEQDPARAKGMVEQL
                     ARWYQQQVQGAAQLKTAAPKFPAQDRLKQEQTKIEQERENLFMEGVRTKVNASVQPEL
                     DRTIEQYTKQYKLNDTQKKYFQDIVQRRVVEELNNDATYKKQVDLRKASKSRTHDTVA
                     SYISGEFNRRLKDAAFTVAKELYGAPKAAVSQNTGVVKAGTPKSTPSGGPIYVSQRPP
                     DAQLDLSRPGAELLLIKGQAYTKDGRFITWRKQ"
     gene            2409..2483
                     /locus_tag="VFA02_14475"
     tRNA            2409..2483
                     /locus_tag="VFA02_14475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2442..2444,aa:Arg,seq:tct)
     gene            2618..3934
                     /locus_tag="VFA02_14480"
     CDS             2618..3934
                     /locus_tag="VFA02_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11108.1"
                     /translation="MAIGAEAAVEAVEIEAFATEIPSLIPLSKTLYSLAQERFQKVPV
                     AFQTQGGATTRPSFRVPFRVQGGAAIAQGTGDGDSLGRGNASVWQDFVLAPVWHYAVN
                     EITHLAQLATNGKKRGLISLKAEELKNSLDSAMAGIEGIMYGDGSGAITQIPTTATVS
                     SGSGTGNQTSFITGVRAMAFTDNQVVQIFPSEGGVARGTATISINDPVTSTLWFSTAL
                     PAGTTTGDYIMVKGSSGAVGSGVIGTTGWINSATSGTVAGINRATYPSRISSPSINLN
                     GGAITASLSQRIEALLGRAMGDSNKTKDSGIYIFGEDQALAVAQTNYYNRQITQNSDP
                     DKNKVPDTSRKYFQGTFGGRDVHISYVQPLGRVDMLLTDDWYIGELVPLQLYDYGGGN
                     TVMPVPDPAGNGWLTSNQFAYEISFNLACSAPRHQLYVYNAAVPTI"
BASE COUNT         1033 a         1067 c         1267 g          755 t
ORIGIN      
        1 gcgcagatgg aacaggcgct ggggcaattg cagcagcagt tcgagcagat gccgaagtat
       61 ctgcccagtg ttcctgtagc gcaggacgag tcagaagacc atgctaccga ggctgcgacg
      121 ctgttttcat ggatgcaggg gccggagggc cggagtctgc gcaaggcggc atcgcgggag
      181 ccggagggcg ggccaaactg gaaaaaatgg acaaattgct acctgcactg gcaggggcat
      241 atgcagatga aggcgaagct ggcagcgcag aatgcgccgc agatgccgcc gaagatgacc
      301 atttcgatcc cggtggacaa gatgcacggc gatgcgcagg cgcaattgct gcaaaaggcc
      361 ggtattcagg tgtctgggcc gaatccagcg ccgcatgagg tggaggaaga ggcgcggatt
      421 tatacgccga catcggagat tgtgcgcaaa cagaagaggc ggctgtgaaa gagcatctgg
      481 tgggctacat tctccggcat ggggaaacag aactgaaccg gaacaactgc tttcgctcat
      541 ggctggacgt gcctttggat gagaacggcc tgagacaggc aaaacaggcg gcggagtatc
      601 tgaaagacaa gccattgaag catgtgattt cgtcgccttt gctgcgggca tttgtaacgg
      661 cggacatggc agcagcgccg cacagattgc aggtctatca gcatcggggc ctgttcccgt
      721 ggcgtttggg catcttcagt ggactcgaaa aagaagaaaa tcaggacgca ttgcggctgt
      781 ttgtgaagaa ccctgcggtg acgattcctg gcggagaatc gctgctggat tttgaggcgc
      841 ggcaattcgc tttctgggaa gcggcgctga agctggcgcg agtccagggc ctgacgctgt
      901 ttgtggcgca cacgagcaat gtggtggcat tgcagaattt tactcaggat ggcgaagacc
      961 ttgagccaga ggatgcagac acggtgaagc caggcggcat tgcagccatt tactggaatg
     1021 gcaaagagca cagagttgaa cctgtcttcg gacaggccga agaagcagta tttggcggaa
     1081 gttagtagac gaagcggaac ggacttgttc cgcaggaaca ggagagacca atggcagatg
     1141 cagtgattga tcttgcagcg cttggagatg acacaggaac aggtagtgcg acggtcgcaa
     1201 cagaccatag cgcggcgaat gagacgcagg aggcatctca gggcgcagag caggatggca
     1261 gccagcagga aagtcagcag caatccgatg ctgagaagct cgacggcagg cgtgggccgc
     1321 aaaacatccg caactcgatc aaggccgctg ccgaagcatt gccagatcat gcgcaggcgt
     1381 tcaaagagct tggcaactca tatttccggg agcaagctta taagcaggtc ttccccacag
     1441 tgcaggaagc gaccacggca aagaacctga ttgattccgt gggcggcctg gacggaattt
     1501 cgcagcttca ggaacgcgat cagctttaca cagcgcagga tgatatgctg cgcgagggca
     1561 atccggaagt attggatgac ttctttgagg actttccgga gggagctgcc gcacttgcac
     1621 cgcattatct ggaccggctc gcgaaaatga atccggaagc gttctccagc gccgtggttc
     1681 cgtatgtggt gggaatgctc gaaaatgccg gcattggcaa ttatctggcc agcatcctcc
     1741 aagaacagga cccggcgcgc gccaaaggca tggtggaaca gcttgcgcgc tggtatcagc
     1801 aacaggtgca gggcgcggca caattgaaga cagcggcccc gaagttcccg gcacaggaca
     1861 gactgaaaca ggagcagacg aagattgagc aggagcggga aaacctgttc atggaaggcg
     1921 tccgcacgaa ggtgaatgct tctgtgcagc cggagcttga ccgcacgatt gagcaatata
     1981 cgaagcaata caagctgaat gacacacaga aaaagtactt tcaggacatt gtgcagcggc
     2041 gcgtggttga ggaactgaac aatgatgcga cctacaaaaa gcaggtagac ctgcgcaagg
     2101 cgagcaagtc gcgcactcat gacacggtgg ccagctatat cagcggggag tttaaccgca
     2161 ggctgaaaga tgcggcgttt accgtggcca aagagttgta tggagcgcca aaggcagcag
     2221 tttctcaaaa taccggagtg gtaaaggcgg gaacaccaaa gtccactcca tcaggagggc
     2281 cgatttatgt atcacaaagg ccgcccgatg ctcaactgga tttgtcccgt cccggagcag
     2341 agttgctgct catcaaagga caggcataca cgaaagacgg caggtttatt acctggcgaa
     2401 agcagtagtg cggcatagct caaaggaaag agcagcggcc ttctaagccg taggtgatgg
     2461 ttcaagtcca tctgccgcaa ccataggaac ttctttaagg catttcgtct cgtggacgtt
     2521 aagcacgctc aagcctgaaa gcgaagttct cccaaaccga gagacctgaa caagccaaac
     2581 atcacagcgc acgggtgtgc gcagggagaa attcatcatg gcaattggag cagaggccgc
     2641 tgtagaggcg gtcgaaatcg aagcatttgc gacagaaatc ccgtcgctga ttccactaag
     2701 caaaacactg tattcactgg cgcaggagcg cttccagaag gtgcctgtag cgttccagac
     2761 ccagggcggg gccacaacac ggccatcctt ccgcgtgccg ttccgcgtgc agggcggcgc
     2821 agccattgcg cagggcaccg gagacggtga ttctctggga cgcggcaatg ccagcgtgtg
     2881 gcaggacttt gttctggccc cggtctggca ttatgcagtc aacgaaatca cgcatctcgc
     2941 gcagcttgcc accaatggca aaaagcgcgg tctgatttca ctgaaggccg aagagctgaa
     3001 aaattctctg gattcggcca tggcaggaat cgaaggcatc atgtacgggg acggttcggg
     3061 cgcaattacc cagattccga ccacggccac agtgtcaagc ggttccggca cgggcaacca
     3121 gaccagtttc atcaccggag tgcgggccat ggccttcacg gacaatcagg tagtgcaaat
     3181 cttcccgtcc gagggcggcg tggcacgcgg cacagcaacc atctccatca acgatccggt
     3241 gaccagcact ttatggttct caacggccct gcctgcaggc acgaccactg gagactacat
     3301 catggtcaaa ggatcgtccg gcgctgttgg ttccggcgtc atcgggacaa ccgggtggat
     3361 caattcggcc acatcgggga cggtggcagg catcaaccgc gcaacctatc cttcccgcat
     3421 ctcttcgcca tccatcaacc tgaatggcgg ggcgatcaca gcgtcgcttt cgcagcgcat
     3481 tgaagcgttg ctgggacgcg cgatgggcga cagcaacaag accaaagact ccgggattta
     3541 catcttcggc gaagaccagg cattggcagt agcgcagacc aactactaca accgccagat
     3601 cacacagaac tcagacccgg acaaaaacaa agtgccggac acttcgcgga agtatttcca
     3661 gggcacgttt ggcgggcgcg atgtgcatat cagctatgtg cagccgctgg gccgggtaga
     3721 catgctgctg accgatgact ggtatatcgg agagctggtg ccgttgcagc tttatgatta
     3781 cggcggcggc aatacagtca tgcctgtgcc tgatccggcg ggcaacggct ggctgacctc
     3841 caatcagttc gcctatgaaa tcagcttcaa tctggcctgc tctgcgccgc gtcaccagtt
     3901 gtatgtgtac aacgccgcag tgccgaccat ctgattcatc ctcctgcgag ggccatgctg
     3961 gttgacatgg ccctttttct taaagaaaga gagaccaatg gaaatccacg acaagcgtaa
     4021 gttcgatggc gaaggcaaca tgattgccat cgcagaggaa cctgtgagcg cggaaaagct
     4081 gggccaactg cttgcagaga ttccgcgtgt atatgaagac cc
//
LOCUS       DASRBV010000401         6239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_295632, whole genome shotgun sequence.
ACCESSION   DASRBV010000401 DASRBV010000000
VERSION     DASRBV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295632"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /locus_tag="VFA02_14485"
     CDS             complement(<1..3026)
                     /locus_tag="VFA02_14485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZU11109.1"
                     /translation="MRRLRILLAFVCLAALPALAQKITGAISGTVTDATGAAVPNATV
                     TITNTATNKVYTVTTDSQGGYTIPELPESSYTVDVKAPNFKESVTKNVVVHVATTTSI
                     NPTLELGSVSESVTVQANAIQVQTDSASLGEVVDGTQVRELPLNGRNFVELTQLQPGV
                     SAANNFQNTGKGLNGGVDMSINGNPTTNNLFLVDGVNNNDVGSNRTILIYPSDDAIAE
                     FKMLTNSYGPEYGQASGGVISIVTRSGSNAFHGTAFYSGRNDALDAYDYFTAPNAGKG
                     LPLDGKAKDRRNDWGYSLGGPIVKDKLFFFWSEEWNHWIKGRQVSACVPTAAERAGDF
                     STLGCNTAGFPDFSKVPASDLAGPNKLNSIDPATATMLQMLPLPNTPGGALNSNGQNW
                     SASLPTGLFWRQENVRVDYDLNHSNHIMGRYTQDTWSNPAFNAGYWGDTVYPALNSNW
                     AQPSKSITAHWTSTISSTLVNDAAFNYSNNRIVITPGGTNPGLLAQVSAAIPTLYSES
                     LKHAKQGVPQVNFGSYGGTTSMQAPWQNELDLYSFRDDLTWVKGQHTLKFGAFLGFNG
                     KNEDVNSASGERPNVGGPATANGGIDTGDSLANLELPGNVFNIGETSTNIRAQLRWRD
                     YEFYAGDTWKLTPKLTLSYGVRYSLLLYPYQPNDQLTSFQPFLYDPSKPASDACNGLW
                     VAPGKDPCGAANAQFGTNFSHGTPGPDRALVKNGYHNFAPRIGFIYDPFGTGKTVFRV
                     GGGQFYQRERVSEYTLVANAPFALTTSAYPRTLGGATPSSISGTASPTGGKDPSPIIP
                     NSWQWNFTVEQQIARNTTFQVGYVGNRGIHLTNVYDVNSIAPQNWLAATFANSGTATN
                     LLRPYPAGGNNGTLTYWSHGGSSNYNGLQVMLQSRVGNALQLGAAYTWSHALADVQID
                     DSSGGIGSQARTYFTNPRLDYGTSDVNRPNMFIANVTYFLPKLQGANVMERGILGGWE
                     ATSIIAAQNGNNFTIFQGAHENVSNLAPNTSSQLNQ"
     gene            3585..4160
                     /gene="pyrR"
                     /locus_tag="VFA02_14490"
     CDS             3585..4160
                     /gene="pyrR"
                     /locus_tag="VFA02_14490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZU11110.1"
                     /translation="MPETQTSQTPVLREKGRIMSASEIERTLVRLAHEIIEKNNGSEN
                     LGLVGVKRRGVPLAQRLGALISNIEKQPIDVGVLDISFYRDDLSTKDVRPVVNPGALG
                     FDVNGRDVVLVDDVLYTGRTVRAAMDALFDHGRPRRVQLLVLIDRGHRELPIEASFVG
                     RTIQTTDREIVEVKLREVDNDEQVVLVERVD"
     gene            complement(4330..4935)
                     /locus_tag="VFA02_14495"
     CDS             complement(4330..4935)
                     /locus_tag="VFA02_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZU11111.1"
                     /translation="MQWLRRWLIAVMFLPVVSHLSAQQVVLGNWQLPFAVTSDTFRDG
                     ATLPLSMIYNSVSNGVNACTADGSAGGNASPQLTWTPYPGARTYAVVLFDETANFTHW
                     GLYNLPPTTISLPADAGIANNNYGPQITNDFGNAEYDGPCPPPGLVHKYVFTVYAVDK
                     KLDLPQSQTFPANAETLFRSLIGHVLAKTSIAGYYSTTPQQ"
     gene            5041..6156
                     /locus_tag="VFA02_14500"
     CDS             5041..6156
                     /locus_tag="VFA02_14500"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZU11112.1"
                     /translation="MGVAIGAPGERVGACRLVKETIRQITINAAHRMTKAKPKPRQTP
                     SSKEQSFSHHPGSLLSITDLSLDAISEILKLATVLEKEDPLKRMKRLFKRRVSLLFYE
                     SSTRTRTSFELAAKQLGADTVLVSALSSSIEKGESLKDTGITLRALGAECIILRHPNS
                     GAPYVLSRVTGLPILNAGDGMHEHPSQALLDLRTILAHVRGAKAAASVSETILKGVTI
                     TICGDIFHSRVARSNMLLLPRLGAHVVLCGPPVLLPETAAAAGPGITVERDFERALKQ
                     SQVVMMLRIQAERLAGLNLDLEEYKLRYQANAERMAAHAPKALIMHPGPIIRGLEITS
                     EVADGPQSAIAEQVRNGVAIRAALLARALTSKGKASR"
BASE COUNT         1353 a         1649 c         1864 g         1373 t
ORIGIN      
        1 ttctggttga gctggctgct ggtgtttggg gccaggttgc ttacgttttc atgcgcaccc
       61 tggaagatgg tgaagttgtt gccgttctgg gcggcgatga tgctggtggc ttcccatccg
      121 ccgagaatgc cgcgttccat cacgttcgca ccctgcagct tcggcagaaa gtaggtcacg
      181 ttggcgatga acatattggg acggttgacg tcagacgttc cgtaatcaag acgagggttt
      241 gtaaagtagg tccgtgcctg tgagccgatg ccgccgctgg agtcgtcaat ctggacatca
      301 gcgagagcat gcgaccacgt ataagctgca cccaactgca gtgcattgcc aactcggctc
      361 tgcagcatca cctggaggcc gttgtagttg gagctgcctc catgcgacca gtaggtaagg
      421 gtgccattgt ttccgcccgc cggataaggc cgcaggaggt tggtggcagt accgctgttt
      481 gcaaaggtgg ccgccagcca gttctgcggg gcgatggaat tcacgtcgta gacattggtg
      541 agatggatgc cgcggtttcc aacgtaaccg acctgaaagg tcgtgttgcg ggcaatctgc
      601 tgttccacag tgaagttcca ctgccatgaa ttgggaataa tgggactcgg gtctttgccg
      661 ccggtggggc tggctgtacc cgaaatactg gaaggtgtgg cgccccccag tgtgcgggga
      721 taagcactgg tggttagcgc gaaaggcgca ttggccacca aggtgtactc ggagacccgc
      781 tcacgctgat agaactgccc cccaccgacg cggaagacag ttttccccgt accgaagggg
      841 tcatagatga agccgatgcg aggagcgaag ttgtgatagc cgtttttgac gagcgcgcgg
      901 tccggtccgg gcgtgccatg actgaagttg gtcccgaact gggcattcgc tgcaccgcag
      961 ggatccttgc cgggagcgac ccagaggccg ttgcaggcat cggaagctgg cttgctcgga
     1021 tcataaagga acggctggaa gctggtgagc tggtcgtttg gctggtaagg atagagcaac
     1081 aacgaataac gcacgccata actcaatgtg agttttgggg tgagcttcca ggtgtcgccc
     1141 gcgtagaact cataatcacg ccagcgtaac tgggcgcgaa tattggttga ggtttcgccg
     1201 atattaaaga cgtttcctgg caattcaagg tttgccagcg aatcgcccgt atcaatgccg
     1261 ccattggcag tggcagggcc tccgacattc ggacgctcgc cactggcgct gttcacgtct
     1321 tcattctttc cgttgaagcc gaggaaagcg ccgaacttca gcgtgtgctg tcctttgacc
     1381 caggtgagat catcgcggaa gctgtagagg tccagttcgt tctgccacgg ggcctgcata
     1441 ctcgtcgtgc cgccgtagga gccgaagttc acctgaggca caccttgctt ggcatgcttg
     1501 agcgattcag agtaaagcgt gggaatcgct gctgagacct gggccagaag acctgggttg
     1561 gttcctccgg gggtgatcac gatgcggttg ttggagtaat tgaatgctgc atcattcacc
     1621 agcgtgctgc tgatggtgga ggtccagtgc gcggtgatgg actttgaggg ctgcgcccag
     1681 ttgctgttca gcgccggata aacagtatcg ccccagtagc cggcattgaa ggcagggtta
     1741 gaccatgtgt cctgtgtgta acggcccata atatggttcg agtggttcag atcatagtca
     1801 acacgaacat tttcctggcg ccagaaaagc ccggtcggca atgaggctga ccagttttgg
     1861 ccattggagt tgagcgcgcc gcccggggta tttggcaacg gaagcatctg aagcatcgtt
     1921 gctgtcgccg gatctattga attcagtttg tttgggcctg ccagatcgga agccgggact
     1981 ttgctgaagt ctggaaaacc tgctgtattg catcccaggg tcgagaagtc tccggcccgt
     2041 tcagctgcag taggcacaca ggcactcacc tgacggcctt tgatccagtg gttccactcc
     2101 tctgaccaga agaagaagag cttgtctttc acgatgggtc cgccgaggct gtaaccccag
     2161 tcattgcggc ggtctttcgc tttgccgtcc aggggcaaac ctttgccggc gttgggagcg
     2221 gtgaagtagt cataggcgtc cagggcgtca ttgcgtccgc tgtagaaggc ggtgccatga
     2281 aacgcgttgc ttccgctgcg ggtaacaatg ctgatgacgc cgcccgatgc ctgtccatat
     2341 tcagggccgt agctgttggt cagcatcttg aactcggcaa tggcgtcatc ggatgggtaa
     2401 atcaggatgg tacggttaga gccaacgtcg ttgttgttga cgccgtccac caggaacagg
     2461 ttgttggtgg tggggttgcc gttgatggac atgtccacgc cgccgttcag gccctttccg
     2521 gtgttctgga agttattggc cgcagaaacg ccgggctgga gctgtgtgag ctcgacgaag
     2581 ttgcgcccgt tcagcggaag ctcgcgcacc tgggtgccgt ctactacttc acccagcgag
     2641 gcgctgtccg tttgcacctg gatggcattg gcctggacgg tgaccgattc actgacgctg
     2701 cccagttcaa gcgtgggatt gatgctggtg gtggtggcca cgtggaccac gacatttttt
     2761 gtgaccgatt ctttaaagtt tggcgccttt acgtccacgg tatagctgga ctcgggcagt
     2821 tcgggaatgg tatagccgcc ctgcgaatcg gtggtgacgg tatagacctt gttggttgcc
     2881 gtattggtaa tggtgacggt ggcgttggga acggccgcgc cggtggcgtc cgtcacggtg
     2941 ccgctgattg caccagtgat cttctgtgca agcgccggaa gggcagcaag acagacgaac
     3001 gcaagcaaaa tgcgaagtcg tctcattgtg tatggcctcc tgaagttggt tcaaaagaca
     3061 gccagatgcc ggttagcatg ggtcctcctg aagatgttta cctgtataga cttacggcct
     3121 ggttaacttc ttgaaacccg caagtcagct gactggggaa atcacccgta aaaacttcat
     3181 gcactgcatg aaatatattt agtaaacggt ttgagtagtg tttttatcgt gaaacaaacc
     3241 gcgctgatta ttaagctcgt ttgatgattc gcttgtcaag cagaaagctg tctcaggggc
     3301 cttaagaatg aacaccaaag gcgggtaaac gcatgattat tctttaatta atcgttttat
     3361 ctcgaaaatg tgccgtaaag tgcgatgtac tttcttgtaa acgattgcaa aatatgcctg
     3421 ctcgaacttg tggggacaga gagcactcaa attccgccca ggatggcaaa acatcgcaaa
     3481 gccgacagag ggacgccgcc ataggtgatt ttttgtccgg gcctggatct gcggcgaggc
     3541 ttgctctgcc ctgccgcgtc ctcgtatcat cggcaagagc ctttatgcca gagacacaga
     3601 caagccagac acccgtgctg cgggaaaagg gccgcatcat gtccgcctca gagattgagc
     3661 ggacgcttgt gcggctggcc cacgagatca ttgagaaaaa caatggcagc gagaacctcg
     3721 gtcttgtcgg cgtgaaacgc cgcggagttc cgctggccca acgcctggga gcgctcatca
     3781 gcaacattga gaagcaaccc atagatgtgg gagtgcttga catcagcttt taccgcgatg
     3841 atctttcaac gaaagacgtg cgccctgtcg taaatcccgg cgccctcggc tttgacgtga
     3901 acggacgcga cgtggtgctg gtggatgacg tcctctacac cggacgcacc gtgcgcgccg
     3961 ccatggatgc cctctttgac cacggaaggc cgcgccgcgt gcagcttctt gttctgattg
     4021 accgtggcca ccgcgagctt ccgatagaag ccagcttcgt cggaaggacc atccagacca
     4081 ctgaccgcga gattgtggaa gtgaagctgc gcgaagtgga caacgatgag caggtagtgc
     4141 tggtggaacg cgtggattaa agcggctact gcgaagatct gaagaaaaat gaccccagag
     4201 gcactcagag agagaacgcc ccggcaagcc ataggaacag gcaccctgct gacaaatgca
     4261 ggcttgtttt tctctgttct tttcgcgcaa tcccagcgcg aaaagaacag agaatcagct
     4321 tctgggcacc tactgttgtg gcgtggttga gtagtagccc gcaatgctgg ttttggccaa
     4381 aacatggcca ataagcgaac gaaacaacgt ttcggcattg gcggggaaag tctgcgactg
     4441 cggtaaatcc agcttcttgt ctaccgcata gaccgtaaag acatacttat gcaccaggcc
     4501 gggaggcggg catgggccgt catactcggc attgccgaaa tcgttggtaa tctgcggccc
     4561 gtaattatta ttggcaatac ctgcatcagc cgggagagag attgtggttg ggggcagatt
     4621 gtagagtccc caatgggtaa agttggcagt ttcatcgaag agcaccacgg cgtatgtcct
     4681 ggctccggga tacggtgtcc acgtaagctg cggagaggca ttgccacctg cgctgccatc
     4741 ggccgtgcag gcgtttacgc cattgctcac ggagttatag atcatactga gaggcagcgt
     4801 ggccccatcg cggaacgtat cacttgtaac tgcaaacggt aactgccagt ttcccaacac
     4861 gacctgctgg gcggaaaggt gcgaaacaac aggaaggaac atgactgcaa tcaaccaacg
     4921 gcgcaaccac tgcatggaaa atgcctcctg agtaagatga agtgcgttca acatagcacg
     4981 cgaaggggcg tgcggtttca aacacggatg aaagtctgcc aagccgcatc ttgagtgtca
     5041 atgggcgtgg caatcggtgc gcccggggag cgtgtcgggg cctgccggct ggtgaaagaa
     5101 accatccggc agattacaat caatgcagcc catcgcatga cgaaagccaa accgaagccg
     5161 cgccagacgc cgtccagcaa agaacaatct ttttcccatc atccaggatc actgctctcc
     5221 atcaccgacc tttcgctgga tgccatttcc gaaatcctga agctcgccac tgttcttgaa
     5281 aaagaagacc cgctgaagcg catgaagcgc ctcttcaagc gccgcgtctc tttgctcttt
     5341 tatgagtcga gcacgcgcac gcgcacctca ttcgagcttg ctgccaagca gctcggcgca
     5401 gatactgtgc tcgtcagcgc gctctcatcg agcattgaaa agggcgagtc actcaaggac
     5461 acgggcatta ccctgcgcgc gctcggggcc gaatgcatca ttctgcggca tcccaactct
     5521 ggcgcaccct atgtgctttc gcgcgtcaca gggctgccca tcctgaatgc aggcgatgga
     5581 atgcacgaac atccatcaca ggctttgctt gacctgcgca ccattctcgc gcatgttcgc
     5641 ggagcgaaag ccgccgcatc tgtttctgag acgatactca aaggcgtgac catcaccatc
     5701 tgcggagaca tcttccacag ccgggttgca cgctcgaaca tgctcctgct gccgcgcctg
     5761 ggagcacacg tggttctctg cggaccgccg gtgctcctgc ccgaaaccgc cgctgctgcg
     5821 gggcctggaa tcacagtgga gcgcgacttt gaaagagcgc tgaaacaatc gcaggttgtc
     5881 atgatgctgc gcatccaggc cgagcggctg gcgggattga atctcgatct tgaggaatac
     5941 aagctgcgct atcaggcaaa tgcagagcgc atggcagcgc atgcgcctaa ggcgctcatc
     6001 atgcacccgg ggcccattat tcgtggactt gaaattacca gcgaagtcgc cgacggtccg
     6061 cagtctgcaa tcgcagaaca agtacggaac ggcgttgcca ttcgcgcagc gcttttagcg
     6121 cgtgccctta catctaaagg aaaagcatca cgatgaaacc aattcttatc cgcggaggcc
     6181 atctgattga tccttcttcg ggcgtggacg ctccgcgcga tctgctgttg aaagatggc
//
LOCUS       DASRBV010000402        11518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_40995,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000402 DASRBV010000000
VERSION     DASRBV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40995"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFA02_14505"
     CDS             <1..1095
                     /locus_tag="VFA02_14505"
                     /inference="COORDINATES: protein motif:HMM:NF027792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HZU11113.1"
                     /translation="DVALLRSLLLEPEHTWGTDTKTWLDFDHYLPKDLSAMLDTKNYK
                     VVEFSWQEKRQDLFDGINRLPASLKTEALDAVRSLDAQPPQLAHGTPHAAEKDIETRH
                     FILGIDPKTGAIRRLRNQATGREWASANHLLALFSYQTLSQQDYAHFFSNYIISNADW
                     AKKDFGKPNIERFGAKSQEWLPSLTELQVSKDAQGHRVLAQLAMHDADASSTGRAAFP
                     KKLFMELFLPNAAPVIYLNFYCFDKPATRMPEALWLTFHPAVSDAKSWVMDKSGEQVS
                     PFDVVEAGSRHLHAVSSEIFCKDRDQSFHIGPLDAPLFAFGEKSPLNFSLAQPDLDKG
                     VHCSLFNNAWGTNYIMWYGENMRFRFILRT"
     gene            1167..1817
                     /gene="pgsA"
                     /locus_tag="VFA02_14510"
     CDS             1167..1817
                     /gene="pgsA"
                     /locus_tag="VFA02_14510"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZU11114.1"
                     /translation="MNLPNSITMGRILCVPLLIWMLSPHFPLRGPNGEQELFTSLFFI
                     LVSISDGVDGYLARRRGQITTIGMLLDPLADKLLITAAFICLVQYTPDIMKPWIAVLV
                     IGREFLVSGLRSIASTEGFTIEASDLGKLKTVIQIVSVVAIILAHRWDYWHFGWFLMP
                     VHPVAVTAVYFMVLVSTISAIDYFWAFWRKIDRATAVRRKRDNDFVLSRRKKNIPA"
     gene            complement(1833..2912)
                     /gene="recA"
                     /locus_tag="VFA02_14515"
     CDS             complement(1833..2912)
                     /gene="recA"
                     /locus_tag="VFA02_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZU11115.1"
                     /translation="MAADDRTRAVELALSQIEKQFGKGSIMRLGSKEAVAPVSTISTG
                     SISFDAALGVGGVPRGRVIEIFGPESSGKTTVTLQIIAEAQKLGGMAAFVDAEHALDP
                     VYAKKLGVDVDNLLVSQPDYGEQALEITEALVRSGAIDVLVVDSVAALVPKAELDGEM
                     GDSHVGLQARLMSQALRKLTGTVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALKF
                     YSSVRIDIRRISAVKEGESVVGARTKVKIVKNKVAAPFREAEFDILYGEGISREGDVL
                     DLAVANNIVEKSGAWYSYAGERIGQGRENVRQYLRDNKETFAKIDAELRQKLKIGAPA
                     PAEVPPVPVNGAAAAQEAVRSVRRQ"
     gene            3143..4117
                     /locus_tag="VFA02_14520"
     CDS             3143..4117
                     /locus_tag="VFA02_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZU11116.1"
                     /translation="MTDIIQPSKVDLVGVGLNATDTLIPLSHYPAPGTKASFRSTTIL
                     PGGQVASTVIACQQWGLQTRYVGKLGDDEAAALHRREFSRAGVEAQIITVPGAASLQS
                     LILLDNNGERTVLNRRDERLALQPEELRREWIIQARALHVDGYDTPAAIRAAQWARDA
                     GIPVIADLDELYPGVETLIENIDYLIVSRDFPCRLMQNDDLEQALRQIQRRFGCLLTA
                     ATLGEDGVLAWDGKQFHYTPAYWVEVADTTGAGDIFHAGFIYGLLQDWPLDRQLDYAC
                     AAAALNCTAIGARGGIRTVQEIEALMATGERHPPAYQAQELKIVTPHL"
     gene            complement(4597..5025)
                     /locus_tag="VFA02_14525"
     CDS             complement(4597..5025)
                     /locus_tag="VFA02_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11117.1"
                     /translation="MLQESAHLRHISAQRIIISSLLPICSHMVAQLMHISAHMEQTLP
                     HMGDMRIMHRMAVWHMSMQSSIMHIISIVMEPFFMHIIMVSMHMLMHMEQSSIHRCMD
                     AGRSIIIADISILLFGMVARSGNLSPTSLLQVSGKQRLVL"
     gene            complement(5111..5374)
                     /locus_tag="VFA02_14530"
     CDS             complement(5111..5374)
                     /locus_tag="VFA02_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11118.1"
                     /translation="MKSRHYAVMTSRNQSTTRLGWKSPSTDEMYCGICMRGMILPEAG
                     AVCPSCGGEVVRTFEVIEGGNLLQKKFSIAVAAEQTGRIASSS"
     gene            complement(5580..6050)
                     /locus_tag="VFA02_14535"
     CDS             complement(5580..6050)
                     /locus_tag="VFA02_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11119.1"
                     /translation="MFAKKSFLMLMLFCLVCSMLRGETPNEKRPVRSPMKIYLRACLT
                     AVKATLAANKDAEVPKSCEAADPAMKKPDLVASSFVVSDGNQVMVLANSTQGEQYAIS
                     TQQDVMKEPAPFQFPEENKPRPRMMGEGCQWFWCCHNEFCCFWIGSGSPICHGI"
     gene            6171..6779
                     /locus_tag="VFA02_14540"
     CDS             6171..6779
                     /locus_tag="VFA02_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZU11120.1"
                     /translation="MSTAKLLLLAGDYVEDYEIMVPFQALQVLGHTVHAVCPDKKAGQ
                     TVRTAIHDFEGDQTYSEKRGHNFALNATFSEVKAEDYDGLVVPGGRAPEYLRLNTKVL
                     EIVRHFANEDKPIAAICHGAQLLAAAGVIQGKRVSAYPACSPEVQLAGGEYVELPMTG
                     AIADGNLVTAPAWPAHPAWLGQFQKVLEAYLAENAQPAGAGR"
     gene            complement(6952..7632)
                     /gene="phoU"
                     /locus_tag="VFA02_14545"
     CDS             complement(6952..7632)
                     /gene="phoU"
                     /locus_tag="VFA02_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZU11121.1"
                     /translation="MTRLRFHKSLDELKEKLLVVAGMAEQSIQRAVEAYRTRDLSICD
                     LVDRGEMAINRLEREIDQMALDLLAMEQPMAIDLRFILSVIKINADLERVGDSAVNIV
                     ERVRDLQAYPVVELPIDIPRMANLAAAMVRKALQAFIEADAKMAESVLAMDDSVDKMN
                     ETIHYALLNVMRETPQHTPQALDALVIARTLERVADHATNIAEDVIFWVQGHDVRHHM
                     TVSDGARK"
     gene            complement(7629..8387)
                     /gene="pstB"
                     /locus_tag="VFA02_14550"
     CDS             complement(7629..8387)
                     /gene="pstB"
                     /locus_tag="VFA02_14550"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZU11122.1"
                     /translation="MGVGIEVSDLNAWYGNNHTLQDINLHIPANQATALIGPSGCGKS
                     TFVRCLNRMHETNPIAKVEGTVRIGGLDVYNDASPVEIRRRVGMVFQRPNPFPTMSIY
                     DNVASGLRLNGFRNRRVLDEVVERSLKHAALWDEVKDDLKKKSGASLSGGQQQRLCIA
                     RALAVDPEVLLMDEPASALDPVSTAKIEDLIFELKSQYTIVIVTHNMQQAARVAERTG
                     FFLSGKLVEFDTTHKIFTNPGDKRTEDYITGRFG"
     gene            complement(8388..9263)
                     /gene="pstA"
                     /locus_tag="VFA02_14555"
     CDS             complement(8388..9263)
                     /gene="pstA"
                     /locus_tag="VFA02_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZU11123.1"
                     /translation="MTARYKAGKVNQWRRFFANHLATGLAVLSTIVVVAPLVAIFIYL
                     LYKGASSLNLNFFTQIPRPVGETGGGMANAIVGSAVLLGLGSLLGVPLGIAAGIYLAE
                     FGRGGKLANFVRFTADVLNGVPSIVMGISVYALIVLPQKHFSAFAGGVALGIMMVPTI
                     TRTTEEMLLMVPNAIREAALGLGVPNWRSVLSITLKTASPGVITGCMLAFARVAGETA
                     PLLFTAFGNNFWSTSVNEPIAALPLQIYVYAISPYDEWHRLAWAGALVLILLIVVSVA
                     LVRYVTTRGVLKGAS"
     gene            complement(9265..10317)
                     /gene="pstC"
                     /locus_tag="VFA02_14560"
     CDS             complement(9265..10317)
                     /gene="pstC"
                     /locus_tag="VFA02_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZU11124.1"
                     /translation="MNLKVQEPRVSGLPVASDPVSRVPPAPNEKQPLSAVRSFLMSRG
                     NSAWSDRAFKGLMLICALSVFFIVALIAWQLIVRSQLTISKFGLSFFFKSAWDPVSGD
                     FGALPFIYGTLVSSLVALVIAVPLAIGVAVFLTEMCPKFLRGPLSFLTELLAAIPSVV
                     YGLWAVFVLVPILREHVNPFLMKTLGWTGVFAGPNFGIGLLAAGVILAIMILPIISSL
                     TREVMTAVPHSQREAVLALGATRWEMIRMGVLRNARIGIVGAIILGLGRALGETMAVT
                     MVIGNRPEISKSLLSLGYSMSSVIANEFSEASDDLYLSALIEIGLALFIVTIVVNILA
                     RLLVWAVTRGAPARVV"
     gene            complement(10519..11490)
                     /gene="pstS"
                     /locus_tag="VFA02_14565"
     CDS             complement(10519..11490)
                     /gene="pstS"
                     /locus_tag="VFA02_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZU11125.1"
                     /translation="MCSAVAGHAQKINGAGATFPYPIYSKWFSEYSAQHPGVQINYQP
                     IGSGGGIRQVTSGTVDFGASDGPMSDEQLKASKVKIVHIPTVLGAVVPIYNLPGVNAE
                     LKFSPDVLADIYLGKITNWSDARIAKDNPGVKLPSTDIIVAHRSDGSGTTYIFTDYLS
                     KVSNDWKNSVGRNTAVAWPKGIGGKGNEGVAGLVRQMPGAIGYVELIYALQNKIPYGY
                     VKNAAGNWVKASIEGVTEAAASVKQMPADYRVSITNAPGKDAYPIASFTWLLIPTPAP
                     NAENGKVIKDFLNWMLDHGQSETSSLYYAPLPENVKAKVKATVAGLK"
BASE COUNT         2566 a         3348 c         3174 g         2430 t
ORIGIN      
        1 gatgttgcgc tattgcgcag ccttctgctg gagccggagc acacttgggg cacagacaca
       61 aaaacatggc ttgactttga ccactatctg ccgaaagacc tctccgccat gctcgacaca
      121 aagaactaca aggtcgtcga gttcagttgg caggaaaagc gtcaggacct cttcgatggc
      181 attaacagat tgcctgcgtc actgaagacg gaagcactcg acgcggtccg cagtctcgat
      241 gcacaaccgc cgcagcttgc ccacggaaca ccacacgccg cagagaaaga catcgaaacc
      301 cggcatttta ttcttggaat agacccgaaa acgggggcca tccgccggct gcgcaatcaa
      361 gccactggcc gcgaatgggc ctcggcaaac cacctgctgg cattgttctc gtatcagaca
      421 ctctcccaac aggactacgc ccacttcttc tcgaactaca tcatcagcaa cgcggactgg
      481 gcaaagaaag attttggcaa gccaaacatt gagcgcttcg gcgcaaaaag tcaggagtgg
      541 ctgccctctc ttacagaact ccaggtcagc aaagatgcac aaggccatcg cgtgctcgct
      601 caactggcca tgcacgatgc cgatgcctcc tccaccgggc gcgcggcgtt tcccaaaaag
      661 ctgttcatgg aattatttct gccgaatgcc gcgcctgtga tttatctgaa tttctactgt
      721 ttcgataaac ctgcgacccg catgcccgag gccctatggc ttaccttcca tccggctgtt
      781 tccgatgcaa agagctgggt aatggacaag tccggagaac aggtttcacc ctttgatgta
      841 gtcgaagcag gaagccggca cctgcacgcc gtttcttctg aaattttctg caaagatcgc
      901 gatcagtcct tccacatcgg accgctggat gcgcctttgt tcgcttttgg agaaaaatcg
      961 ccgctgaatt tttcgcttgc ccagccagac cttgacaaag gagtgcattg cagccttttc
     1021 aacaatgcct ggggcacgaa ttacatcatg tggtatggcg aaaatatgcg cttccgcttc
     1081 attttgcgga cgtgaattcg gggtatgcca tgaatctggg ctgtggtgca taattgaagg
     1141 agagcgcact tctcgccggg cgtgccgtga atcttcccaa ttccattacg atgggccgca
     1201 tcttgtgcgt gccgctgctt atctggatgc tctcgccgca cttccctctg cgcggaccga
     1261 acggcgagca ggaactgttc acatccctct tcttcattct ggtttcgatt tccgacggcg
     1321 tagacggcta tcttgcgcgg cgccgcggac agattacaac catcggaatg ctgctcgatc
     1381 cccttgcaga caaactgctg attaccgcgg ccttcatctg tctcgtccaa tacacgccgg
     1441 acatcatgaa accctggatc gctgtactgg tgattggccg cgaatttctg gtgagcggac
     1501 tgcgcagcat cgccagcacc gaaggcttta cgattgaggc tagcgacctg ggcaagctga
     1561 agaccgtcat ccagattgtc tccgttgtgg ccatcatcct ggcgcaccgc tgggactact
     1621 ggcacttcgg atggttcctg atgccggtgc atccggtggc ggtaacagct gtctacttca
     1681 tggtgctggt ctcgaccatt tccgcgattg actacttctg ggccttctgg cgcaagattg
     1741 accgcgctac cgctgtgcgc cgcaagcgag acaatgactt cgtcctgagc cgcaggaaaa
     1801 agaacattcc cgcctgaatg cgctgctgaa tcctactgcc gtcttacgct ccgcacggct
     1861 tcctgcgctg cggccgctcc gttgaccgga acaggcggga cctccgccgg agcaggagcg
     1921 ccgatcttca gcttctggcg cagttcggca tcaattttgg cgaaagtttc cttattgtcc
     1981 ctgagatact ggcgaacgtt ctcacggccc tgaccaatgc gctcgccggc atagctgtac
     2041 caggcgccgg atttctcgac gatattgttc gccaccgcaa ggtccagcac atcgccttca
     2101 cgcgagatgc cttcgccgta cagtatgtcg aactcggctt cgcggaaggg cgcagccacc
     2161 ttgttcttta caatcttgac cttcgtacgc gcgccgacaa cactttcgcc ttcctttacc
     2221 gccgagatgc gccggatatc aatgcgcacg gatgaataaa acttaagcgc acggccaccg
     2281 gtggtcgttt ccggattgcc gaacatcacg ccaatttttt cgcggatttg attaataaag
     2341 atgaggcagg tgcgcgattt tgaaacagtt cccgtgagtt tgcgcagtgc ctgcgacatc
     2401 aaacgggcct gcaaacctac gtgcgaatcg cccatctcgc cgtccagttc tgcttttggg
     2461 acgagcgcgg caaccgagtc cacaaccagc acatcaattg cgccggatcg caccagagcc
     2521 tcagtaattt ccagcgcctg ctcgccgtaa tcaggctggg aaaccagcag gttgtccacg
     2581 tctacgccaa gctttttcgc atagaccgga tcgagcgcat gttcggcatc cacgaaagca
     2641 gccatgccgc ccagcttctg cgcttcggcg atgatttgaa gcgtaaccgt ggttttgccc
     2701 gaagattcag ggccgaaaat ctcaatcaca cggccgcgcg gcactccacc tacgccaagc
     2761 gcagcgtcaa aggagatcga gccggtggaa atagttgaaa cgggggccac ggcctctttg
     2821 ctgccgaggc gcataattga gcctttgcca aactgcttct cgatctgcga aagcgccagt
     2881 tctactgcgc gggtacggtc atctgctgcc acaatacttc tccagttccg gatttttggc
     2941 gcatttcacg ccggaatgcg ccgctctcgt tctgtaaatt gcacggattt gaggcgattt
     3001 tagcactcat cttcgcacgg gcgaataaaa atcaaaaact atagtaacgt ctggcccatg
     3061 gccgggtcta ttgccgcccg caaaattctg aggaaaacag gacaaaaacc atcgtttcct
     3121 ttgagcatgt acattaggaa aagtgacaga catcatccag ccatccaaag tggacctggt
     3181 gggcgttggg ctgaacgcca ccgacacgct gattcctctc tcacactatc ctgctccagg
     3241 gactaaagca tcttttcgtt ccaccaccat tctgcctggc ggacaggttg ccagtaccgt
     3301 catcgcctgc cagcagtggg gactgcaaac gcgctatgtc ggcaagctgg gcgatgacga
     3361 agccgcggcg ctgcaccggc gtgaattttc ccgcgccgga gtggaagcgc agattatcac
     3421 ggtcccaggg gccgccagcc tgcaatcgct gattctgctg gacaacaacg gcgagcgcac
     3481 cgttctgaac cggcgcgatg agcggcttgc gctgcaaccg gaagagcttc gccgcgagtg
     3541 gatcatccag gcccgcgccc ttcatgtaga cggatacgat acgcctgcgg ccattcgtgc
     3601 tgcgcaatgg gcgcgcgatg ccggaatccc ggtgattgcc gatctggatg agctgtatcc
     3661 cggcgtcgaa acgctcatcg aaaatattga ttacctaatc gtaagccggg acttcccttg
     3721 ccgcctgatg cagaatgatg atctcgaaca ggcccttcgt cagattcagc gccgcttcgg
     3781 ctgcctgctg accgctgcca ctctgggaga agatggcgta ttggcctggg acggcaaaca
     3841 gttccactac actcccgcct attgggtcga agtcgccgat accaccggcg caggagacat
     3901 cttccatgcc ggatttattt atggcctgct gcaggactgg cccctcgacc gccagttaga
     3961 ctacgcctgc gcagccgctg ccctgaactg caccgccatt ggcgcccggg gcggcatccg
     4021 gactgtgcag gaaatcgagg ccctcatggc tacaggagag cggcatccac cggcatatca
     4081 ggcgcaggaa ctgaaaatcg tgactccgca tttgtgacgt gtgaaaagcg aaggggcgat
     4141 ttctcttgcg agagaccgcc ccttctatcc gattttctga agacctggct cggcatccag
     4201 gttacaactc actgccgctc cgggagggcc ggaaccagca gaccttgcgg cccgcttgtc
     4261 tcaatgcctt tccagtgcgc cattcgttgc acaggctttc cggatccatc cgcaggcgat
     4321 cagtttcctg attgctttca cgagagccgt ttccggtact caaaggatgc ccttactcgc
     4381 cctcttctcg ctggaagatt tctcttccgg tgatttgaag catactcctg ttcagtccga
     4441 atgcaagggg tctgagaaga ttttgttctg tggaaatagc ggtcattctg tggagaacgc
     4501 aaccactctg gtgaatctgt tcggtttacg cttttctgcc tgcacaatgt tttgtgcttg
     4561 tttcgcaggt ggcctcggca gattgacccc cacaaatcac aaaacaagtc gctgtttgcc
     4621 tgatacctgg agtaagctgg taggcgagag attgccggat ctcgcaacca ttccaaatag
     4681 gagaatcgag atgtctgcaa tgatgatgga acgccctgcg tccatgcagc gatgtatgga
     4741 tgattgttcc atgtgcatga gcatgtgcat ggaaaccatg atgatgtgca tgaaaaaagg
     4801 ctccatgact atggagatga tgtgcatgat ggaagactgc atggacatgt gccagactgc
     4861 catgcggtgc atgatgcgca tgtcgcccat gtgcggcaag gtctgctcca tgtgcgctga
     4921 gatgtgcatg agctgcgcaa ccatgtgcga gcagatggga agcaaagacg agatgatgat
     4981 gcgctgcgcc gaaatgtgcc ggaggtgcgc ggactcctgc aacatgatga tgaaggaaat
     5041 gaaagcggct taatatccga gtctgggcag gtgccggcct gcccagaccg gcagatggcc
     5101 tgatggccgc ctacgaggac gacgcaatcc tgccagtctg ctccgctgca acagcaattg
     5161 agaatttctt ctggagcaga ttccctccct ctatcacttc aaaagtgcgg accacctcgc
     5221 ctccgcagga cgggcacact gctccggctt ccggcaaaat catccctctc atgcagatgc
     5281 cgcagtacat ctcgtccgtg gatggagact tccatcccag acgggtagta gactggttgc
     5341 ggctggtcat gactgcataa tggcgtgact tcatgttgtg atttctccgg aatctgcatg
     5401 aaaaaataaa acggcccacg atctgctcgt gggccgttga ggaaccttat gcgttggaaa
     5461 atcagctaca cggtcctctg cgcccacatg catcacaaca catgcacatc aaacacatgg
     5521 cgctgagggt gctgaaccgc atattgataa gataccctcc gagggctgaa gttgcaagat
     5581 tatattccgt ggcagatggg cgagcctgac ccgatccaga agcagcagaa ttcattgtgg
     5641 cagcaccaga accactggca gccctcgccc atcatccggg gccggggctt gttctcttcc
     5701 ggaaactgga aaggcgccgg ctctttcatt acgtcctgct gtgtggaaat agcatactgt
     5761 tcgccctgtg tgctattggc cagcaccatg acctgattgc cgtcggaaac tacaaacgag
     5821 ctggccacta ggtctggctt tttcatcgca gggtcggctg cttcgcagct cttcggtact
     5881 tccgcgtctt tgttggcggc cagggtcgcc ttcacggccg tcaggcacgc acgcaggtag
     5941 attttcatcg gtgagcggac gggccgcttt tcattcggcg tttcgccgcg aagcatggaa
     6001 cacacaagac agaacaacat cagcatcaga aatgactttt tagcaaacat gctccccctc
     6061 aggtgcgata cggcgtatca cggcgttatg gtgagagaag gcccggagcg tgacaacttt
     6121 ttccggacaa gcgtcataag aatacagacc acggtttgga ggattcagta atgagcacag
     6181 caaaattgct gttgttggca ggcgactacg ttgaggatta cgagatcatg gtgccgttcc
     6241 aggcactgca ggtgttggga cataccgtcc acgccgtctg tccggacaag aaagccggac
     6301 agaccgtgcg cacggccatc cacgactttg aaggcgacca gacgtattca gaaaaacgcg
     6361 ggcacaactt cgccctgaat gctacgtttt ctgaagtcaa ggcagaggac tacgatgggc
     6421 tggtcgtccc cggaggccgc gcgcctgagt acctgcgtct gaacacaaag gtgctggaga
     6481 tcgtgcggca cttcgcaaac gaagacaagc ccatcgctgc catctgccac ggagcgcagt
     6541 tgctggctgc cgccggagtc atccagggaa aacgagtgag cgcgtatcct gcgtgctcgc
     6601 ctgaagtgca gctggcaggc ggagaatatg tcgagctgcc catgaccggt gccattgccg
     6661 acggaaacct ggtgactgcc cctgcatggc ccgcgcatcc ggcatggctg ggccagtttc
     6721 agaaagtcct cgaagcgtat ctggcggaga acgcacagcc ggccggagca ggaagataac
     6781 gagctgagtg accggacgcc cgggagggtt ccaatggcct tttgcaaagt ctggcccccg
     6841 ttccatcaaa aaaggggaga gcggcagtca gcgatgcgaa aggcccgcat caggctggaa
     6901 ttccatattt gaatgtggct tccggaccaa tacggagcaa ggtcaccccg gttacttcct
     6961 tgcgccgtcg cttacggtca tgtggtggcg cacatcgtgt ccctgcaccc agaaaatcac
     7021 gtcttcggcg atgttcgtgg catggtcggc tacgcgctcc agggtgcggg caatcaccag
     7081 cgcatcaagc gcctgcggag tgtgttgagg cgtctcgcgc atcacattca gcagcgcgta
     7141 gtggatggtt tcgttcatct tgtctacgct gtcatccatt gccagcacag attcggccat
     7201 cttcgcgtca gcttcaataa aggcttgcag ggccttgcgc accatggcgg cggccagatt
     7261 ggccatgcgc ggaatgtcta tgggaagctc gacgaccgga tacgcctgga gatcgcgcac
     7321 gcgctcgacg atgttgaccg cggaatcccc gacacgctct aggtccgcgt tgattttgat
     7381 gacggagagg ataaaccgca ggtcaatggc catgggctgt tccatggcga gcaggtcgag
     7441 cgccatctga tcaatctcgc gctccaggcg gttgatggcc atctcgccgc ggtccaccag
     7501 gtcgcagatg gaaagatcgc gcgtgcggta tgcctcgacg gcgcgctgaa tggactgctc
     7561 ggccattccg gccacgacca gcagcttctc cttcaactca tcgaggcttt tgtggaaacg
     7621 taggcgcgtc atccgaacct gcctgtgatg taatcttccg tcctcttgtc gccgggattt
     7681 gtgaaaatct tgtgggtagt atcaaactca acaagtttcc cgctcaaaaa gaaaccagtg
     7741 cgctcggcca cacgcgctgc ctgctgcatg ttatgggtca caatcacaat cgtgtattgg
     7801 gacttcaact cgaagatgag gtcttcaatt tttgccgtgg aaacagggtc gagggccgat
     7861 gctggctcgt ccatgagcag cacttccggg tctacggcaa gcgcgcgggc aatgcagagg
     7921 cgctgctgct gtccaccaga aaggcttgcg ccggacttct tcttcaggtc gtctttcact
     7981 tcgtcccaca gggcagcgtg tttgagagag cgctcgacga cttcatcgag aacgcgccgg
     8041 ttgcgaaagc cgttcagccg caggcccgac gcaacgttgt cgtaaattga catcgtggga
     8101 aacgggttcg gacgctggaa aaccatgccg acgcggcggc ggatttcaac gggagaagcg
     8161 tcgttataaa catccagacc gccaatccgt accgtgcctt cgactttggc gatggggttg
     8221 gtctcatgca tgcggttcag gcagcgcaca aaggtggact tgccacatcc cgaagggccg
     8281 atgagcgccg tcgcctgatt cgctggaata tgcagattga tgtcctgcaa cgtatggttg
     8341 tttccatacc aggcgttcag gtccgatact tcaattccaa cacccactta acttgctccc
     8401 ttcaaaacgc cgcgcgtggt gacgtaacgc accagcgcaa cggagacgac aatcaaaaga
     8461 attaaaacca atgcgcctgc ccaggcaagg cggtgccact cgtcataggg cgaaatggcg
     8521 tagacgtaaa tctggagcgg cagcgcggca atcggctcat ttacgctggt gctccagaaa
     8581 ttgttgccga atgcggtgaa caacaaaggc gcggtttctc cggcaacgcg ggcaaacgcg
     8641 agcatgcagc cggtaatcac accgggcgag gccgttttca gcgtgatgga gagcaccgag
     8701 cgccagttgg gcacgcccag ccccagggcc gcttcgcgga tggcattcgg caccatcagc
     8761 agcatctctt ctgtggtgcg cgtgatggtc gggaccatca tgatacccag cgccactccg
     8821 ccggcaaagg ctgaaaaatg cttctggggc agaacgatga gtgcgtagac ggaaatgccc
     8881 atcacgatgg atggcacacc gttgagcacg tcggcagtga agcggacgaa gttggccagc
     8941 ttgccgccgc ggccaaactc cgccagataa attccggcag caatgcccag aggaacaccc
     9001 agcaggctac ccagtcccag cagaacagcc gagccaacga tggcattcgc catgccgccg
     9061 ccggtttcac ccacaggcct gggaatctgc gtgaagaaat tcaaattcag cgagcttgcc
     9121 cccttgtaga gcaggtagat gaagatggcc accagagggg caacgacgac aatggtgctc
     9181 agaacagcaa gcccggtcgc cagatgattg gcgaagaagc gccgccactg gttcactttt
     9241 ccggctttat agcgcgctgt catgctacac caccctcgcc ggagcgccac gcgtcaccgc
     9301 ccacaccagc aggcgggcga ggatgttcac gacaatcgtc acaatgaaga gcgccagtcc
     9361 aatttcaatc agcgcgctta gataaaggtc gtcggacgcc tcgctgaact cgttggctat
     9421 cacgctggac atgctgtatc caagcgacag cagcgacttt gaaatctccg gccggttacc
     9481 aatgaccatg gtgacggcca tcgtctcgcc gagggcgcgt cccagcccga gaataatggc
     9541 gccaacgatg ccgatacgcg cattgcgcag cacgcccatc cggatcatct cccaccgggt
     9601 ggcgcccagc gccagcaccg cttcgcgctg cgaatgcggg acggcagtca tgacttcccg
     9661 cgtgagcgag gagatgatag gcagaatcat aatggcgaga atcaccccgg cggcaagcag
     9721 gccaatgccg aagttcggcc cggcgaacac acccgtccag cccagtgtct tcatcagaaa
     9781 tgggttgacg tgttcgcgca gaatcggcac cagcacaaag acggcccaca gtccgtagac
     9841 gacgctggga atggcggcca gcagctctgt caggaacgac aagggcccgc gcaggaactt
     9901 cgggcacatt tcagttagaa agaccgctac gccaatcgcc aacggcacgg ctatgaccag
     9961 cgcgaccagc gaggagacca gcgtcccgta aataaacggc agcgcgccaa aatcgccgga
    10021 aaccgggtcc catgcgctct taaaaaagaa gctcaggcca aactttgaaa tggtaagctg
    10081 cgaacgtacg ataagctgcc atgcaatgag cgcgacaata aagaagacgc tcagcgcaca
    10141 gatcagcatc agccccttga aagcgcgatc gctccatgcc gagttccccc gcgacatcag
    10201 aaatgaacgc acggccgaca gcggctgttt ttcattcggc gcaggaggta ctctcgacac
    10261 aggatcggaa gctacaggca gaccggacac acgaggttcc tggactttaa gattcaccac
    10321 agaatacgct aaatgtcttt tttgaatatc acgttaaacg ctttgaaaaa taagaagtaa
    10381 atcttatcga aaatgggggt tttgccaccc ggaaagcctg aaatgaatga atggagtacg
    10441 cagaatctgt gaaactcggc aggatggccg tttcctttcc tgcggaaacg gccctttccg
    10501 gccatccggt tccaggactt acttcaaccc ggctacagtc gcttttacct ttgcctttac
    10561 gttttcgggc agcggagcgt aatagagcga gctggtttct gactggccat ggtcgagcat
    10621 ccagttcagg aagtctttga tcacctttcc attctctgcg ttcggcgctg gggtcggaat
    10681 gagtagccag gtgaaactgg caatcggata agcatccttt cccggagcat tggtgatgga
    10741 aacccgataa tcggccggca tctgcttcac gctggcagcc gcctcggtca cgccttcaat
    10801 gctggccttc acccagttcc cggcggcatt cttcacataa ccatagggaa ttttgttctg
    10861 cagggcataa atcagttcga cataaccgat cgcccccggc atctggcgga caagtccagc
    10921 cacgccttca ttgcccttgc caccgattcc tttcggccag gccacagccg tattgcgtcc
    10981 tacggagttc ttccagtcat tgctgacctt tgacagatag tcagtaaaaa tataggtcgt
    11041 gccgctgcca tccgaacgat gggcaacaat aatgtctgtg ctcggcagct ttacgcccgg
    11101 attgtccttg gcaatgcgcg cgtcgctcca gttcgtgatt tttccaagat agatgtccgc
    11161 cagaacatca ggcgaaaact tcagttcggc attcactccg ggcaggttat aaatcggcac
    11221 aactgcgccg agcaccgttg gaatgtggac gatcttcacc ttcgacgcct taagctgctc
    11281 atcgctcatc ggcccgtcgc tggcgccaaa gtccaccgtg ccgctggtca cctggcggat
    11341 accgccgccg cttccaatgg gctggtagtt aatctggacc ccgggatgct gggcgctgta
    11401 ttcgctgaac cattttgagt aaatcggata cggaaaggtc gcgccggccc cgttgatctt
    11461 ctgggcatga ccggcaaccg ctgaacacac aaccaacgca gcagccaata acgtgctt
//
LOCUS       DASRBV010000403         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_557143, whole genome shotgun sequence.
ACCESSION   DASRBV010000403 DASRBV010000000
VERSION     DASRBV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557143"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFA02_14570"
     CDS             <1..609
                     /locus_tag="VFA02_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZU11126.1"
                     /translation="YWFKWGTVCGLPNTLIARTGYTGEDGFEIYIPSDEATSKRVWEE
                     VLEAGREFGILPCGLGARNTLRLEAALSLYGHEISETINVFEAGLDRFCKLEKGPFIG
                     SDALQKVLAGGGPERRLVGLEVVERGIARDGYCVFNADGKQIGAVASGSPAPFLKKNI
                     ALAFVPREYAAPDTEVFVQVRSAMVKAKVVPTPFYRRPKKQQ"
     gene            640..1029
                     /gene="gcvH"
                     /locus_tag="VFA02_14575"
     CDS             640..1029
                     /gene="gcvH"
                     /locus_tag="VFA02_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZU11127.1"
                     /translation="MAYPANYRYTREHEWIEADGKTGRVGITDYAQNSLGDIVFVESP
                     KVGASVKKGEVFGSVESVKAVSDLYSPVTGTVTEVNEELASAPEKINSDAHSAWIMKV
                     ELSDPAELESLLSAADYEKYIAEETGH"
     gene            1131..2477
                     /gene="gcvPA"
                     /locus_tag="VFA02_14580"
     CDS             1131..2477
                     /gene="gcvPA"
                     /locus_tag="VFA02_14580"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZU11128.1"
                     /translation="MRYLPKSPAERAEMLREIGASSIDDLFSVIPEEYRLQRDLKIPR
                     QMGESEIVDHFKNAAAHNAVGYTSFLGAGVYRHYRPVIIDSLVQRGEFLTSYTPYQAE
                     ITQGTLQAIFEFQTMICELTGMDIANASMYDGSTGAAEAVMMAVRITGRDGVVVARTV
                     HPEYREVMETYSQHQEHSAQEVGYDADGRVDLAALDQAVTEKTACVLVQSPNFFGIVE
                     DIPAIAEIAHKKGALLIVSIAEAVSLGIVRPPVEADIVSLEAQSFGVASSYGGPFCGV
                     LAAKETYLRQMPGRLVGETKDKDGRRGFVLTLSTREQHIRREKATSNICTNQALVALM
                     ATIFMTVYGREGIKELALQNLAKAQYAARKLGESGKLLFAGSPRFNEFVIETSEAPEQ
                     MNARLLENKIIGGVPLKKWYPELGNATLWCATELTAKEQIDQAAAVLAETAVATTA"
     gene            2569..>3746
                     /gene="gcvPB"
                     /locus_tag="VFA02_14585"
     CDS             2569..>3746
                     /gene="gcvPB"
                     /locus_tag="VFA02_14585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZU11129.1"
                     /translation="MATTEQPFTGTPRKVTAHITQNEKLSFELSSPGKKAYKLPPLDV
                     PEADPAQVLGEAARESTGALPELSEIEIIRHFTRLSTWNYAIDLGMYPLGSCTMKYNP
                     RVNEFVSRLEGIAEAHPYQPEPLAQGSLQIIKLLQDCLIEITGMDAITLQPAAGAHGE
                     FTGILLIRAYHKSKGNTRKNILIPDSAHGTNPATAAVCGYQVVNIKSNAQGGIDVAAL
                     EQIVDEDTAALMLTNPSTLGVFENEIHKIADILHAKGALLYMDGANMNALCGKTRPGD
                     FGVDVMHLNLHKTFSTPHGGGGPGSGPVACKKILEPFLPVPVVEQGSDGQLHWNYDRP
                     ESVGRVRAFYGNFGMFVRALAYILANGPDGLRQTTEDAVLNANYIRKKLEDVYELPYK
                     T"
BASE COUNT          835 a          951 c         1187 g          773 t
ORIGIN      
        1 tactggttta agtgggggac ggtctgcggc ctgcccaata cgctgattgc ccgcacggga
       61 tacacgggcg aagatggctt tgagatttac atcccttcgg atgaagcgac gagtaagcgc
      121 gtatgggaag aggtgctgga agcgggcagg gaattcggca ttctgccgtg cggcctgggg
      181 gcgcggaaca cgcttcgtct ggaggcggcg ctgtcgcttt atgggcatga gatttctgag
      241 acgatcaacg tctttgaagc cgggctggac cgcttctgca agctggaaaa ggggccgttt
      301 atcgggtcgg acgcgttgca gaaggtgctg gctggcggcg gcccggagcg caggcttgtc
      361 gggctggaag tggttgagcg cggtattgcg cgtgatggtt actgcgtatt taacgcggac
      421 ggcaaacaga ttggcgcagt ggccagcggc tcgcctgcgc ctttcttaaa aaagaacatt
      481 gcgctggcct ttgttccgcg cgagtacgcg gcgccggaca cggaagtttt tgttcaagtg
      541 cgcagcgcga tggtaaaggc gaaggtcgtg cccacgccgt tttaccgccg gcccaagaaa
      601 cagcaataac agaccgtaca atagaaagag aaaacctaca tggcatatcc tgcgaactac
      661 cgctataccc gtgaacacga gtggattgaa gctgatggca agacaggcag ggtcggcatt
      721 accgattacg cccagaactc gctcggcgat attgtttttg tggaatcgcc gaaggtggga
      781 gcgtcagtga aaaaaggcga ggtctttggc agcgtggagt cggtgaaggc cgtttctgat
      841 ttgtattcgc ctgtgaccgg gaccgtgact gaagtgaatg aagaactggc ctccgccccg
      901 gaaaagatca acagtgatgc tcactcggcc tggatcatga aggttgagct gagcgatccg
      961 gcagagctgg aatcacttct gagcgcggcg gactacgaga agtacatcgc ggaagaaacg
     1021 ggacactagt ttcgttgtca gttgtcagtc cccagtcttc ggtgagtacg ctcctgggga
     1081 ctggagactg accactggtc ttgctgggga cttttttacc aaggaacatc atgcgttatt
     1141 tacctaaatc gcctgctgaa cgcgcggaga tgctgcgcga gataggcgcg tcttcgattg
     1201 acgatctctt ttcggtcatt ccagaggaat accgtctcca gcgcgacctg aagattccgc
     1261 gccagatggg cgaatcggaa atcgtagacc actttaaaaa cgcggctgcg cacaatgctg
     1321 tgggctacac cagctttctt ggtgcgggtg tgtatcgtca ctaccgcccg gtcatcattg
     1381 attcgctggt gcagcgcggc gagtttctga cttcgtatac gccctatcag gccgagatta
     1441 cgcagggcac gcttcaggcc atcttcgagt tccagacgat gatctgcgag ctgaccggaa
     1501 tggacattgc caacgcttcg atgtatgacg gctcgaccgg cgcggcggaa gcggtgatga
     1561 tggcggtccg cattaccgga cgggacggcg tcgtggtggc gcggacggtg catccggagt
     1621 accgcgaggt gatggagacc tactcgcagc accaggagca ttcagcgcag gaagttggct
     1681 atgacgcaga cggacgcgtg gatttagcgg cgctcgacca ggcggtaacg gagaaaacgg
     1741 catgtgttct ggtgcagtcg ccaaatttct ttgggattgt cgaagacatt ccggcgattg
     1801 ccgaaatcgc acacaaaaaa ggcgcactgc tgattgtttc gattgccgag gcggtatcgc
     1861 tggggattgt gcgtcctccg gtggaggcag acatcgtttc tctggaggcg cagtcatttg
     1921 gtgtggcgtc cagctatggc gggccattct gcggagtgct tgcggcgaag gaaacgtatc
     1981 tgcggcagat gcccgggcgg cttgtcggag agaccaaaga caaagacgga cggcgtggat
     2041 ttgtgctgac gctttcaacg cgcgagcagc acatccggcg cgaaaaagcg acctcgaaca
     2101 tctgtacgaa ccaggcactc gtggcgctga tggcgaccat ctttatgacg gtctacggca
     2161 gagagggaat caaagagctt gccttgcaga acctcgccaa agcgcaatat gcggccagga
     2221 aacttgggga aagcggcaag ctgctctttg ccgggtcgcc gcgcttcaac gaatttgtga
     2281 ttgagaccag cgaagcgccg gagcagatga atgcacgcct gctcgaaaac aagatcattg
     2341 gcggagtgcc gctgaagaag tggtatccgg agctgggcaa cgcgacctta tggtgcgcga
     2401 cggagctgac ggccaaagag cagattgacc aggcagcggc agtgctggcc gaaacggctg
     2461 tggcgactac ggcttaagag acaacccgca agaacgcggg cgggcagcag agttttgcag
     2521 cgatcatttg ctgctttttc gggtctttca cgattgacag gaattttgat ggcaacaacg
     2581 gaacaaccat ttacgggaac gccgcgcaag gtcacggcgc acatcacgca gaacgagaag
     2641 ctctcgtttg aattgtcttc tccgggaaag aaggcctata agctgcctcc gctggatgtc
     2701 ccggaggcag acccggcgca ggtgctgggc gaagcggcgc gtgagagcac aggcgctttg
     2761 ccggagctga gcgagatcga gatcatccgt cacttcacgc ggctctcaac gtggaactac
     2821 gccattgatt tggggatgta tccgctgggc agttgcacga tgaagtacaa cccgcgcgtg
     2881 aatgagtttg tctcgcgcct tgagggcatt gccgaggcgc atccgtatca gccggagccg
     2941 ctcgcgcaag gctctctgca gattatcaag ctgttgcagg actgcctgat tgaaattacc
     3001 gggatggatg cgattacgct ccagccggca gcaggggcgc atggcgagtt taccggaatc
     3061 ctgctgatac gcgcatacca caaatcaaaa ggcaatacgc gcaagaatat tctgattcct
     3121 gactcggcgc atgggacgaa ccctgcaact gctgcggtct gtggctatca ggtagtgaac
     3181 atcaagtcga acgcgcaggg cggcattgat gtggccgccc ttgaacagat agtggatgaa
     3241 gataccgccg cgctgatgct taccaaccca tctacgcttg gtgttttcga aaacgaaatt
     3301 cacaagattg ctgatattct ccatgccaaa ggcgcgctgc tgtatatgga cggcgcaaac
     3361 atgaacgcgc tctgcggcaa gacacgtccg ggtgatttcg gcgtagatgt gatgcacctg
     3421 aacctgcaca agacattttc aacgccgcat ggcgggggag ggcctggctc cggtccggtc
     3481 gcctgcaaga agattctgga gccgtttctg cctgtgcctg ttgtggagca aggaagcgat
     3541 ggccagttgc attggaacta tgaccggcca gagtccgttg gacgtgtgcg cgccttctac
     3601 ggcaacttcg ggatgtttgt gcgcgcgctt gcgtacattc tggcgaacgg cccggatggt
     3661 ctgcgccaga ccacagaaga cgcagtgctg aatgcaaact atatccgcaa aaagctcgaa
     3721 gatgtttacg aactgcccta taagac
//
LOCUS       DASRBV010000404         8691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_297101, whole genome shotgun sequence.
ACCESSION   DASRBV010000404 DASRBV010000000
VERSION     DASRBV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297101"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..3326
                     /gene="bamA"
                     /locus_tag="VFA02_14590"
     CDS             471..3326
                     /gene="bamA"
                     /locus_tag="VFA02_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZU11130.1"
                     /translation="MPESAGSPVKTSKLRRMLKPLAFILLCFTVFDVWAQSSQTITQI
                     RVIGNRRIPKETILARLFSHEGEQFDPTTVERDFNSLWNTGYFEDVRIEREDNPKGGI
                     ILDIYVREKPTIREINYKGLNSVTQSDVLDRLKKEKVGFSVESQYDPTKIARVETVLK
                     ELLSEHGRQFATVKADVKTIPPASVQVNFIIKEGPKVKVGNISFTGNQHIPSRDLRRA
                     MKNLRPIGIPHSIFLEDLFAKTYDASKLEEDAERVRQAYRDKGYFRASTGDPQTHLRN
                     EGGLSFFTFRPRKGKRIDIRIPVEEGARYRLGYIHFTGSKLQNLNVNALRAQFPLKDG
                     EWFNFTAFGKGLQNLQKAYSTLGYINFTANPIPKIDDDKKIVSFDVELDEGKQFYVSR
                     IEFQGNTVTRDFVIRRELYLEEGQVYNSHLWELSLLRLNQLDYFDPLKVDQDTEVHQN
                     PETGTVDLLLKVHEKGKNSIGLNGGVSGLSGTFLGLNYQTNNFLGLGETLSVQASAGN
                     LSRNLLFSFNEPYIRNKPINAGFSIYSQKYDYNASKAYNLSGADTNTQQAVNSLVQNY
                     NQSTTGFTVSAAYPLRHSFKRLGFTYALNRSSVTTFSQASQNLFQTLAFRSGIQGQNS
                     LEGIVNSMASFSFSWNKLDSVYMPRSGSELNTIFQLAGLWGNVRYFSPIVEFKQFKPI
                     KWFRFNNDGRNVFGYRIQANYIQGFSGDVAPPFNRFYGGGEADVRGFDIRTLSPYGFV
                     PTRVQFNLTNPDGNCVPQDPTNPQTNQCIKVPIPVYGISSIGGDTQIVGNMEYRIPLV
                     GRTAALELFNDFGMVMAVNNSQLKQSPQGYDQLNAPLYGCPNYVNGACVGGQQIQFDR
                     YIRPIPGTNYVPRDSIGAQISVMVPIINAPFRIYYAYNPLRLHTQFNGQNLITRSMFP
                     AGGAGDYSYAQAIQLYGSIYQLREPTKTFRLTVSTTF"
     gene            complement(3396..4088)
                     /locus_tag="VFA02_14595"
     CDS             complement(3396..4088)
                     /locus_tag="VFA02_14595"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HZU11131.1"
                     /translation="MTLRGEIGEDGQGRTNPIRQPMLVAGTLIGVAGIGLQFHFMMQV
                     PELSTTEAVARFFSYFTILTNLLVLMGYAFPLLAPGSSIGKFFSRAPMRGATLVYIVM
                     VGVVYSLLLRNLYHPAGWAKAADVLVHDAAPVYYAVFWFLCAEKSGLRPSHAAQWLLY
                     PLAYIVYTLLRGVVTDWYPYPFVDVRVLGYSRTFIHAGVLALVFLIAGLGVVAAARWL
                     SARRAAAEQAGA"
     gene            4325..5398
                     /locus_tag="VFA02_14600"
     CDS             4325..5398
                     /locus_tag="VFA02_14600"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HZU11132.1"
                     /translation="MALPRFALSLLLTLFLASATLAQADHPGYLQQAKDGIQTLQSWY
                     TQSSGLYQTTGWWNSANAITVLADYSRVAHTKDYLPVFSNTFTQAQSSHAGFINDYYD
                     DEGWWALAWVAAYDLTHQPQYLSMASSIFSDMANGWDSTCGGGIWWSKAKTYKNAIAN
                     ELFLSVAAHLARRVPIDQRAQYLDWAQKEWAWFKNSGMINSQHLINDGLNLSTCQNNQ
                     QNTWTYNQGVILGGLTELNKVAPDPSLPATAQAIANAALTHLTDANGILHDTCEPNCG
                     ADGVQFKGIFVRNLMALYEAFPLPQYKKFADTNAESIWQHSQGPNYEFGQVWSGPFDA
                     ANAGTQSSALDAIVAAATMQSQH"
     gene            complement(5619..6503)
                     /locus_tag="VFA02_14605"
     CDS             complement(5619..6503)
                     /locus_tag="VFA02_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU11133.1"
                     /translation="MRVFVTGATGFIGSALVPELVRAGHEVLGLARSDAGVQALTAAG
                     VQAHRGDLTDIESLRSGAAAADAVIHLAFVHDFSKFRENCETERKAIEAMGAALKGPD
                     CPLIVTSGLGVLAKGRPATEDDAPLPLSDQYPRASEHTAVALAEKGVKAMVVRLPQVH
                     DTRKQGLISSAIQIAREKGVAAYVGEGQNRWAAVHVSDAARLYRLVLEKGRAGARYHA
                     VAEEGFSAREIAEVIGRGLKVPVVSLKPEEAAAHFGWFARFAQWDLTGSSEKTRKELN
                     WSPTGPGLITDLENMRYF"
     gene            6624..7208
                     /locus_tag="VFA02_14610"
     CDS             6624..7208
                     /locus_tag="VFA02_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU11134.1"
                     /translation="MPQKRPQPAPRKLRTDAQRNRERILEVAKEAFTRSGAGASLDDI
                     ARQAGVGPGTLYRHFPTRDALIEAVYRNEVEKLARAAYSFAETMPPVEALRAWMLLFI
                     EYIAAKHIIAPALNSVGGGPSRLYEGTRPLVQDAIDMLAKRAIRSGEFRKDIEPFDLL
                     RALIGVSLIGMAPDWQKSARRLVDVLIAGSRPAQ"
     gene            7237..8004
                     /locus_tag="VFA02_14615"
     CDS             7237..8004
                     /locus_tag="VFA02_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU11135.1"
                     /translation="MEKFARYPSLNGKVVLITGGGTGIGASLVEHFAHQGAKVSFLDV
                     AEEASKEWVSRIGKSTGHAPLFFPCDLTDISALQAAIQEAEAALGPIDVLVNNAANDE
                     RHKWEEVTPEYWDRMMDVNLRHQFFAIQAVAPGMKARGSGSIINLSSISWIIPGTGLP
                     AYIAAKAAIVGLTRTMSRELGPSGVRVNCVLPGAILTEKQRRLWWTPDYEQKIMSSQC
                     LKRHLMPEDVARLVLFLASDDSAAITGQSHIVDGGWV"
     gene            complement(8008..8664)
                     /locus_tag="VFA02_14620"
     CDS             complement(8008..8664)
                     /locus_tag="VFA02_14620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZU11136.1"
                     /translation="MSKKKWAAVDNYIDSILGATDPALDAALEAVRAAGLPQISVTAS
                     QGKLLHLLAKLQGAKRILEIGTLGGYSTIWMARALPKNGKLVTIEADAKHARVAAENI
                     ARAGLSEKVELRKGKALSVLPELEGPFDLIFIDADKRNNPRYFEWALKLARKGSLIIV
                     DNVVREGEILDADSADPDIRGTRKLFELMAAEPRVMAMAQQTVGSKGHDGFAIALVTG
                     "
BASE COUNT         1953 a         2757 c         2207 g         1774 t
ORIGIN      
        1 ccaaacgacg aatggcccta tggccgcgtg gttgccgtag aagaagaccc gcttgaaacc
       61 cgcgccaatc gccttcaggt acgcaggaac atcgaaacta ccatgcaggt gctcaataac
      121 ctgggcgtcg ttgtctatga atggccctca ggcagccccg gcaacagata gacttctact
      181 ccacatcccc aaaatccctt ctatccgata gactaagaac ggatttccaa atgccaagac
      241 accggccagg gaagcagcag gccggagtgt ggcggttttt gtggagattg tatggtttac
      301 tgcaaaccaa atcttctctg aaccgtcgga atggtgatgg ctccagaatg actgggaagg
      361 cggtctaaag atgctccggg gttgggaaca ctgcctccgg tcacttataa tcgctttttc
      421 ctcaatattt gatctgactt tgcagtgagg gtgtgcctat ttttcccgtc atgccagaga
      481 gcgcaggctc tccagtaaaa accagcaagc tccgccgcat gttgaagcca ttggccttca
      541 ttctcctttg cttcacggtc tttgatgttt gggcgcagtc gtcgcagacg atcacacaga
      601 ttcgcgtgat cggcaaccgt cgtattccca aggaaaccat cctcgcccgc ctcttcagcc
      661 atgaaggcga acagtttgac cccactacgg tggaacgcga ctttaactcc ctctggaaca
      721 ccggatactt cgaagatgtc cgcatcgaac gcgaagacaa tcccaagggc ggcatcattc
      781 tggacatcta tgtccgggaa aaacccacca tccgtgagat caactacaaa ggactgaatt
      841 cggtcacgca gtccgacgtc ctcgaccggc tcaagaaaga aaaggtcggc ttctctgtcg
      901 aaagccagta cgaccccacc aaaattgccc gggtcgaaac agtgctgaag gagctgcttt
      961 ccgagcatgg ccgccagttc gccacagtca aagccgatgt aaagaccatc cctccggcct
     1021 ccgtgcaggt gaacttcatc attaaggaag gcccgaaggt caaagtcggc aacatcagct
     1081 ttaccggcaa ccagcacatt ccgtcacgcg atctgcgccg ggcgatgaag aacctgcggc
     1141 cgattggcat cccccattcc atctttcttg aggacctctt cgcgaagacc tatgatgcca
     1201 gcaagctcga agaagacgcc gagcgtgtcc gccaggccta tcgcgacaag ggttatttcc
     1261 gcgcctccac cggagaccca cagacacatc ttcgcaatga aggcggcctg tcgttcttta
     1321 ccttccgtcc gcgcaaaggc aagcgtattg acatccgcat cccggtagag gagggtgcgc
     1381 gctatcgtct gggctatatc cacttcacag gaagcaagct ccagaacctg aatgtgaacg
     1441 ccctgcgcgc ccagtttccg cttaaggatg gcgagtggtt caatttcacc gcctttggca
     1501 agggcctcca gaacctgcag aaggcctaca gcaccctggg ctacatcaac ttcaccgcca
     1561 atccgattcc gaaaattgat gacgataaga aaatcgtttc cttcgacgta gagctggatg
     1621 aaggcaagca gttctatgtc tcgcggattg agttccaggg caataccgtc acccgcgact
     1681 ttgtcatccg ccgcgaactg tatcttgagg aaggccaggt ctacaacagc catctgtggg
     1741 agctgagcct cctgcgcctg aaccagctcg attacttcga cccgctcaag gtagaccagg
     1801 acaccgaagt ccaccagaac ccggaaacag gcacggtaga cctgctgctc aaagtgcatg
     1861 aaaaaggcaa gaactccatc ggcctgaatg gtggtgtcag cggcctgtcc ggaaccttcc
     1921 tcggcctgaa ttaccagacc aataacttcc tcggcctcgg tgaaacgctg agcgtgcagg
     1981 ccagcgccgg aaacctctcg cgcaacctgc ttttcagctt taacgagccg tatatccgta
     2041 ataagcccat caacgcgggc ttttccattt attcgcagaa gtacgattac aacgcgtcga
     2101 aagcatataa cctgtcgggt gcggacacga atactcagca ggcggtgaac tcgctcgtcc
     2161 agaactacaa ccagtccaca accggtttca cagtttctgc ggcatacccc ctccgccact
     2221 cgttcaaacg cctcggcttc acctatgcgc tgaaccgctc atcggtcacc accttcagcc
     2281 aggcctcgca gaacctcttc cagacactgg ccttccgcag cggcatccag ggtcagaact
     2341 cgcttgaagg cattgtgaac agcatggcct ccttcagctt ctcctggaac aagctggatt
     2401 cggtctacat gccgcgctcc ggctcggagt tgaacaccat cttccaactt gccggactct
     2461 ggggcaatgt tcgctacttc agccccattg tcgaattcaa gcagttcaaa cccatcaagt
     2521 ggttccgctt taacaacgac ggccgcaacg tcttcggata tcgcatccag gcaaactaca
     2581 tccagggctt cagcggagac gtggctcctc ccttcaaccg cttctatggc ggcggcgaag
     2641 ccgatgtgcg cggattcgac atccgcacgc tctcgcctta cgggttcgtt cccacgcgcg
     2701 tccaattcaa cctcaccaac ccggatggca actgcgtccc gcaggacccg accaatccgc
     2761 agaccaatca gtgcatcaag gtgccgattc cggtctacgg catcagctcg attggcggcg
     2821 acacccagat tgtcggcaac atggaatacc gtattcccct tgtgggccgc accgccgcgc
     2881 tggagctgtt caacgacttt ggaatggtca tggccgtcaa caacagccag ttgaagcaga
     2941 gcccgcaggg ctacgaccag ctcaatgctc cgctctatgg ttgccccaac tacgtcaacg
     3001 gggcctgcgt cggcggacag cagatccagt tcgaccgcta catccgcccc atccccggca
     3061 caaactacgt gccgcgtgat tccatcggcg cgcagatttc ggtcatggtg cccatcatca
     3121 acgcgccatt ccgcatctac tacgcctaca acccgctgcg ccttcacacg cagttcaatg
     3181 gccaaaacct gattacccgc agcatgttcc ccgccggcgg agcaggcgat tacagctatg
     3241 cccaggccat ccagttgtac ggttcgatct atcagttgcg cgaacctacc aagaccttcc
     3301 gcctgacggt aagcactacg ttctgacgga atatgcgcaa tctctacgcg gtgccttcaa
     3361 aaaaggcgcc gcgtttttat ttgccgggaa cgggatcagg cacccgcctg ctccgccgca
     3421 gcccggcgcg cagacaacca cctggccgcc gcaaccacgc cgagccctgc tatcagaaaa
     3481 acaagcgcga gcacgcctgc gtggataaag gtccgtgagt agccgagtac cctgacatcc
     3541 acaaaaggat agggatacca atctgtcaca acaccgcgca gcagggtgta cacgatatag
     3601 gccagcggat agagcagcca ttgcgccgcg tggctgggtc gaagaccgga tttctctgca
     3661 cacaaaaacc agaagacagc gtaataaaca ggggctgcat catgcaccag aacatccgct
     3721 gccttcgccc atccggcggg atgatagagg ttccgcaata gaagcgaata caccacgccc
     3781 accatcacga tatagaccag cgtagctccg cgcatcggcg cgcgcgaaaa gaacttgcct
     3841 atagaggagc cgggcgccag cagcggaaac gcgtacccca taagcaccag caggttcgtc
     3901 agaatcgtga agtaactgaa gaagcgcgcc acggcctccg ttgtcgaaag ctcggggacc
     3961 tgcatcatga agtgaaactg tagaccaatc cccgcaacac caatcagggt gcctgcgacg
     4021 agcatgggct gtctgatggg attcgtcctg ccctgcccat cttctccaat ttctccccga
     4081 agtgtcactt tccctgcatg ttacagcatt cgtatatgca gagtcctctc attctgacga
     4141 ccgaagggag gaagaatctc agcgatgtga gggttgagcc actcctgctt tctcgcgcct
     4201 ccccggaata atgatcaatc tttttcgtgg attcctgcaa aacgaaaact ggaagaattg
     4261 acatcctgca atactctgtc ctaccctgtc tccgctgatg gaaacgttcc cacaggagac
     4321 atgcatggcc ctcccccgct tcgccctctc gctcttgctc acactgttcc ttgcgtcggc
     4381 cacgctcgcg caggcagatc atcccggcta tcttcagcag gccaaagacg gcatccagac
     4441 cttgcaatcg tggtacacgc aaagcagcgg cctttatcag acaaccggat ggtggaactc
     4501 cgccaacgcc atcaccgtgc tggctgacta ttcgcgcgtg gcccatacaa aggactatct
     4561 gccggtattc tccaacacgt ttactcaggc gcagagcagc catgcaggct tcattaacga
     4621 ctactacgac gatgaaggct ggtgggccct ggcctgggta gctgcctacg acctgaccca
     4681 tcagccgcag tacctctcca tggcgtcctc catcttttct gatatggcga atgggtggga
     4741 ctccacttgc ggaggcggca tctggtggag caaagcaaag acctataaga acgccatcgc
     4801 caacgagctt tttctctctg ttgccgcgca cctggcgcgc cgcgtgccaa tagatcaacg
     4861 cgcgcagtat ctggactggg cgcaaaaaga gtgggcgtgg tttaaaaact ccggcatgat
     4921 caactcgcaa cacctcatca atgatggact gaatctctct acctgccaga acaatcaaca
     4981 gaacacctgg acctacaacc agggcgttat cctcggcggc ctgacagagt tgaacaaagt
     5041 cgcgcccgat ccttcccttc ctgcaacagc acaggccatt gccaacgccg cgctcacgca
     5101 tctgaccgat gccaacggca ttctgcatga cacctgcgag cccaactgcg gcgccgacgg
     5161 tgtgcaattt aagggcatct ttgtgcgaaa tctgatggcc ctctacgagg cgtttccgct
     5221 tccgcagtac aaaaaatttg cagacacaaa cgctgagagt atctggcagc attcccaggg
     5281 accgaattat gaatttggcc aggtgtggtc aggccccttc gacgccgcca atgcaggaac
     5341 ccaaagctcg gcactcgatg ccattgtggc cgcggccaca atgcaaagtc agcattgaga
     5401 acaacccatc gtcggccctg aaatttacgg atgctccaga ggcgtccctc ttttccgtcc
     5461 cgttgtgcgc ggtgtggaga gttcgtgatt ctgacggccg aaggagggaa gaaccctcag
     5521 cgatgtcagg ccttaaccaa tcctgcagaa ctcttccgca ccgcccggaa cggcgatgag
     5581 acttttcgtg aaacaaaaaa atgttcaggc cttcttattc agaaatagcg catattctca
     5641 agatcggtga tcaatcccgg cccagtcggg ctccaattca gttcttttcg tgtcttctcg
     5701 ctggaacccg taaggtccca ctgcgcgaac cgtgcgaacc agccgaaatg cgctgcggcc
     5761 tcttcaggct tcagagaaac cactggcacc ttcagcccgc gtccgatcac ctcggcaatc
     5821 tcccgtgccg aaaagccttc ttcggccacc gcgtggtagc gcgccccggc gcggcccttc
     5881 tccagaacga gccggtagag ccgggccgcg tcgctcacat ggaccgcggc ccagcggttt
     5941 tgtccttcgc caacataggc cgcaacgcct ttttcgcggg caatctggat ggcggagcta
     6001 atcagtccct gcttcctcgt atcgtggacc tgcggaagac gtacaaccat cgccttcacg
     6061 cctttttctg ccaacgcaac cgcggtgtgc tcagaggcgc gcggatactg atcagaaaga
     6121 ggaagaggcg catcgtcctc cgtggcagga cgtcccttcg caagcactcc aagaccgctg
     6181 gtaacaatca atggacagtc tgggcctttc agcgcggccc ccatggcctc gatggccttc
     6241 ctctctgtct cacagttctc cctgaacttt gaaaaatcgt gaacaaacgc aagatggatc
     6301 accgcatctg ctgccgctgc tccactgcga agactctcga tgtccgtcag atcgccacgg
     6361 tgtgcctgca ctcccgcagc ggtcaatgcc tggacgcctg catcgctgcg cgccaggccc
     6421 aacacctcat gtcctgcgcg cacgagttca ggcaccagag cagaaccgat aaaacctgtc
     6481 gctcctgtta caaaaacacg catatgtccc tccttttgct gctgttcgtt gaaagccttt
     6541 gcctgtcgcc gcatacaata tatgaggaga cagtctccgg ttgaatagat aaacggagac
     6601 tgtctccggt tgcagatttt tttatgcctc aaaaacgccc acaacccgct ccgcgaaaac
     6661 tgcgcaccga tgcccagcgc aaccgcgagc gcattctgga ggtcgcaaag gaggcattca
     6721 cccgttccgg cgccggtgcc agcctggacg atatcgcgcg gcaagccggc gtggggcctg
     6781 gaaccttgta ccgccacttt cccacacgcg acgctttgat tgaggccgta taccggaatg
     6841 aagtcgaaaa gctcgcccgc gccgcctaca gcttcgctga aaccatgcct ccggtcgagg
     6901 cgctgcgggc ctggatgctg ctgttcattg aatacatcgc cgccaagcac atcattgctc
     6961 ccgccctcaa cagcgtcggc ggtggccctt cccggcttta cgaaggcacg cgccccctgg
     7021 tccaggatgc gatagacatg ctggcaaagc gcgccatcag gagtggcgag tttcgcaaag
     7081 acatcgagcc gtttgatctg ctgcgggctt taatcggagt ctctctgatt ggcatggcac
     7141 ctgattggca gaagagcgcc aggcggctgg tggacgttct catcgcaggc tcccgtccgg
     7201 cgcaataaag aagcattcct gtaccatgct tcggagatgg aaaagttcgc gcgctacccc
     7261 agtcttaacg gcaaagttgt cctcatcact ggaggcggga ccggcatcgg tgcctctctt
     7321 gtcgaacatt tcgcccatca gggcgcgaaa gtgtcctttc tggatgttgc cgaggaggcc
     7381 tcaaaagaat gggtgtctcg aattggaaaa agcaccggac acgctccgct ctttttcccc
     7441 tgcgacctga cagacatctc cgccctgcaa gctgccattc aggaggcaga agcggcgctt
     7501 ggcccaatag atgttctcgt caacaacgcc gccaacgatg aacgccacaa atgggaagaa
     7561 gtaacacccg aatattggga ccgcatgatg gatgtcaacc tgcgccatca attctttgcc
     7621 atccaggcgg tcgcgccggg aatgaaggcg cgcggcagcg gttccatcat caatctcagc
     7681 tccatttcat ggatcatccc cggcaccgga cttcccgcct acatcgccgc caaggccgcc
     7741 atcgtcggcc tcacgcgcac catgtcgcgc gagctgggcc catcgggtgt ccgcgtcaat
     7801 tgtgtgctgc cgggcgcaat cctcacagaa aagcagcgcc gcttgtggtg gaccccggac
     7861 tacgaacaga aaatcatgtc cagccagtgc ctcaagcgcc atcttatgcc tgaagatgtg
     7921 gcccggctgg tcctctttct ggcatcggac gattctgcgg ccatcaccgg ccagagccat
     7981 atcgtggacg gaggctgggt atgaacgtca gccagtcacc agcgcaatcg caaagccatc
     8041 gtgtcccttg ctgccgaccg tttgctgtgc catcgccatc acgcgaggct cggcagccat
     8101 caactcaaac agctttctgg taccacggat gtccgggtct gcgctgtctg catccagaat
     8161 ctctccttcg cgcacaacat tgtccacgat aatgaggctt cccttgcgcg caagcttcag
     8221 cgcccactca aaataccgcg gattgttgcg cttgtccgca tcaataaaga tgaggtcgaa
     8281 aggcccttcc agttcaggca ggacgctcag cgccttcccc ttacgcagtt ccaccttttc
     8341 agacagtccg gcgcgcgcaa tgttctcagc cgccacacgc gcgtgtttcg catccgcttc
     8401 gatggtgacc agctttccgt tcttcggcaa agcgcgggcc atccagatcg tgctgtagcc
     8461 gcccagtgtc ccaatttcca gaatgcgctt tgcgccttgc agcttcgcca gcagatgcag
     8521 cagcttaccc tggctcgcag tcacgctgat ctgcggcagc cctgccgccc ggacagcttc
     8581 gagcgccgca tccaatgcag ggtctgtagc ccccagtatg gaatcaatat agttgtctac
     8641 tgccgcccat tttttctttg acataccgca tcattctccc acagcctgct c
//
LOCUS       DASRBV010000405         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_349774, whole genome shotgun sequence.
ACCESSION   DASRBV010000405 DASRBV010000000
VERSION     DASRBV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349774"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFA02_14625"
     CDS             complement(<1..219)
                     /locus_tag="VFA02_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU11137.1"
                     /translation="MNKLVLGNVLHRPLRTIIGVFAVAVEVIMILSIVGIMIGQVSNA
                     RNQTGGIGADMIVRPPNASFVTGVGGAPV"
     gene            517..1056
                     /locus_tag="VFA02_14630"
     CDS             517..1056
                     /locus_tag="VFA02_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU11138.1"
                     /translation="MGQFGEDLRKERESRGVALETITDATKISSRHLLALEQEHFDIL
                     PGGVFNKGIVRNYARVVGLDEDFWVERFMSAYQQSGLLKDDDANWIEFAENVRKNRPE
                     DTARPGQRLRWTGVVVLLVLLSVLGWFVWRYVSKRLAASNEHPAPSESAALLVPGPLF
                     PQLASQALKRSNHVLRPAL"
     gene            complement(991..3156)
                     /locus_tag="VFA02_14635"
     CDS             complement(991..3156)
                     /locus_tag="VFA02_14635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZU11139.1"
                     /translation="MKILSNWLRSYLPELGVTDRQLADDLTLRGIAVEGVFDLGPNGS
                     LFEMDITTNRVDAMNHYGIAREAATIYGLELKKLDTTLPQGKPTAKPFPVSIEEPALC
                     GRFTARVLRNVKITPSAGIVAERFRLLEQKPISNAVDATNYVTLAMGHPTHAFDLDKL
                     EGGIILRRARKGEKLRTLDGIERTLDPDDLVVADEKKALAIAGVMGGWDSMITPETKN
                     ILVEAAWFDPWTVRRSSRRHLLHTDASHRFERGADFNAPPVASALVSSIILESGGEVE
                     GDLVDVIIPEAEARTAKRPAISFALSEAHRILGATEDPHGITAETAETILAGLGCKLK
                     KNGTPHVVPAQDLDPSRAVSTEAAPVRAEYEVTLPSWRLDLEREIDLIEEIARVYGYN
                     RFRNTLPSFAGAVVELPTAEKEAVVRRTLLALGWTEAISSTFCSATDAVTFAPQPNSA
                     VPMDNPLSEEAGMLRTSLAPGMLSMLALNLNRDVEDVMLFEMGAVFSGSTDRVEERPA
                     LAIGATGSLPLEGPHQPARALDFYDIKGCVEELLSKFAAKLLYFDRFPSGSGLMPSWL
                     HPGRSARAVIDGATIGYFGEVHPAEAQRRKLRQTVYIGEFSLDRLYKQDLRQPAMREL
                     SRFQAVRRDFSFIFADAVSYAQIAAALASLNIAELQRFEPKEVLRDREGKRIPAGHFS
                     LLLRTVFQAQDRTLQEEELQGWSQDVIAALQGLGGKLRE"
     gene            complement(3187..3600)
                     /locus_tag="VFA02_14640"
     CDS             complement(3187..3600)
                     /locus_tag="VFA02_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZU11140.1"
                     /translation="MKQVSALQASQPVSPKTGARTRHYRDLLVWQKAMQLARNVYSHT
                     TGFPKSEVFGITSQMRRASLSIPSNIAEGHGRLSDGSFRVFLAHARGSLYELETQIEL
                     ARDLTYLQADHAKELLEQCQEIGRMLNGLIGVLES"
     gene            complement(3597..>4640)
                     /gene="pheS"
                     /locus_tag="VFA02_14645"
     CDS             complement(3597..>4640)
                     /gene="pheS"
                     /locus_tag="VFA02_14645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZU11141.1"
                     /translation="FAELAREVESSAQALHSPESVEQFRLQWLGRKQGRLKLISDAWL
                     KSSPAEAKKPLGQRFNQLKAQIEERLERASGASAQFALDAEAIDVTLPGTRRALGAEH
                     PLIKTMHEMVAVFKRMGYSAGVGPEVETDYYNFESLNFPPNHPARDTQDTLVIANQER
                     RPLRDRLLMRTHTSPVQIRTMEQQPPPVRIVIPGKVHRNDEADATHSPIFHQIEGLCV
                     DTNITFSDLKGTLDHAMKEFFGSSVKTRFFPSFFPFTEPSADVQISCPFCGGKGCRKC
                     KHSGWIELLGCGMVDPAVFGFVQQKQPAYDPKKISGFAFGMGVDRIAMMKYGISDIGL
                     LYSGDTRFLEQFA"
BASE COUNT          934 a         1261 c         1469 g          976 t
ORIGIN      
        1 tacgggcgcg cctccaacgc cggtgacaaa gctggcgttg ggcgggcgca cgatcatgtc
       61 agcgccgatg cctccggtct ggtttctggc attggagacc tgcccgatca tgatgccgac
      121 gatggagagg atcatgatga cttctacggc gacggcgaag acgccgatga tggtacgcaa
      181 ggggcggtgc agcacattac cgaggacaag cttgttcatg ggagtcctga aaagaggaag
      241 cccaccgttc catcctggga cagcggcgaa tctctataaa atttgagtgt aacaaagtga
      301 aaactgctgc cgataatcga aaaaacggct gaagggccat ttcgagaccc gaaaaagtgc
      361 cagtttcagg cagaaatggc ctgatttggc tttgactttg gacagcggga gttttatggt
      421 ttgcactaga ggttgggcaa gagtccggcc ttgtatgacg gatgatttga agtgaaattt
      481 tgagatccca gagagtacct gcatcgagac agtggaatgg gccagttcgg cgaagattta
      541 cgcaaagaac gggaatcgcg cggcgtcgcc cttgaaacga taacggatgc cacaaagatc
      601 agcagccgcc acctgctggc tctggagcag gagcactttg acatcctgcc cgggggcgtc
      661 ttcaacaagg gaattgtgcg taattatgcc cgtgttgtcg gcctggacga ggatttctgg
      721 gtggagcggt tcatgtctgc gtaccagcaa agcgggcttc tgaaagatga tgacgccaac
      781 tggattgaat ttgcagaaaa tgtgagaaaa aaccgtccgg aggacacggc acgcccggga
      841 cagcgccttc gctggactgg ggtggtggtg ttgctggtgc tgctctctgt cctgggatgg
      901 ttcgtgtggc gctatgtcag caagcgcctc gctgcttcaa acgaacatcc ggccccgtct
      961 gaatctgctg ctcttctggt tcccggtccg ctattcccgc agcttgcctc ccaggccctg
     1021 aagcgcagca atcacgtctt gcgaccagcc ctgtaattcc tcttcctgca aggtacggtc
     1081 ctgcgcctgg aaaacggtgc gcagcagcag tgagaaatgt cctgccggaa tgcgcttgcc
     1141 ttctctgtcg cgcaggacct ccttcggttc gaagcgttgc agttcggcga tgttgagcga
     1201 tgcgagcgca gcggcgatct gcgcgtaaga gactgcatcg gcaaagatga aggagaagtc
     1261 gcgccggacg gcctggaagc gcgacagctc acgcatggca ggctggcgca ggtcctgttt
     1321 gtagaggcgg tcgagagaga actcgccgat gtagacggtc tgcctgagct tgcggcgctg
     1381 tgcttctgcc ggatgcacct cgccgaagta accgatggtg gccccatcta tcacggcgcg
     1441 cgcggagcgg cctggatgca gccaggacgg catcagacca gagccggatg gaaaacggtc
     1501 aaagtagagc aattttgcag cgaacttgct cagcagttct tccacgcagc ccttgatgtc
     1561 gtagaagtca agcgcgcgcg caggctggtg cgggccttcg agcggcaagc tgcccgtggc
     1621 gccgatggca agcgcgggcc tttcctccac tctgtccgtt gagccggaga agacggcgcc
     1681 catctcaaag agcatgacgt cttccacatc gcggttcagg ttgagtgcga gcatcgaaag
     1741 cattcccgga gcgagtgatg tacgcaacat tccggcctct tcgctcagcg gattgtccat
     1801 gggtacagcg gaatttggct gtggggcaaa tgtaactgca tcggttgcag aacagaaggt
     1861 gctggagatg gcctccgtcc agcccagtgc caggagggtg cgacgcacga ccgcttcttt
     1921 ctccgcagtg ggcaactcga cgacggcccc ggcgaaggaa ggaagcgtgt tccggaagcg
     1981 gttgtatcca taaacgcggg cgatttcttc gatcaaatca atctcgcgtt caaggtcgag
     2041 ccgccagctt ggcagcgtga cttcatactc cgcgcgcacg ggcgctgctt cggtggaaac
     2101 ggcacgcgaa ggatcaaggt cctgtgccgg gacaacgtgc ggtgttccat tcttcttcag
     2161 cttgcagcca agcccggcaa ggatggtctc agcggtttcc gcggtgatgc cgtgcgggtc
     2221 ttccgtcgcg ccgaggatgc gatgggcctc gctcagagcg aagctgatgg ccggacgctt
     2281 cgcggtgcgt gcttcggctt caggaatgat gacatctacg agatcgcctt cgacttcgcc
     2341 gccgctttca agaatgatgc tgctgaccag cgctgaagca accggcggag cgttgaagtc
     2401 ggccccacgc tcgaagcgat gcgaagcatc ggtatgcaga aggtggcgcc tggagctgcg
     2461 gcgcaccgtc cagggatcga accacgcagc ttcaacgaga atgttctttg tctcgggcgt
     2521 aatcatcgag tcccacccgc ccatgacgcc ggcgatggcg agcgcttttt tttcgtcggc
     2581 cacgacgaga tcgtccgggt cgagcgtgcg ttcaatgccg tccagggtgc gcagcttctc
     2641 gcccttgcgc gcgcggcgca ggatgatgcc gccttcgagc ttgtcgaggt caaaggcatg
     2701 cgtgggatgg cccatcgcca gcgtgacgta gttggttgcg tccactgcgt tggagatggg
     2761 cttctgttca agcaggcgaa agcgctcggc gacgatgccg gcagatggcg tgatctttac
     2821 attgcgcagc acgcgggcag tgaagcgtcc gcagagggcg ggttcttcga tggagacggg
     2881 gaacggtttt gccgtgggtt tcccctgcgg caatgtggtg tcgagcttct tcagttccag
     2941 cccataaatg gtcgcggctt cacgtgcgat gccgtagtgg ttcatggcat ccacgcggtt
     3001 ggtggtgatg tccatctcga agagagagcc attcgggccg agatcgaaga caccttcgac
     3061 agcaatgcca cgcagcgtga gatcgtcggc gagttggcgg tcggtcacgc cgagttcggg
     3121 caaataggag cggagccagt tggagagaat tttcatagcg agtcagcggg tcagcgggtc
     3181 agcgagtcag ctttccagaa ctccaatgag tccgttcagc attctgccga tttcctgaca
     3241 ttgctctagt aattcctttg catgatcagc ctgaagataa gttaagtcac gggccagttc
     3301 aatttgtgtt tccaattcat atagcgagcc gcgtgcgtga gccagaaaga cacggaaact
     3361 cccatcagaa agacgtccgt gtccttcagc aatattgctg ggtattgaca aggatgcgcg
     3421 acgcatttgg ctcgttatcc caaatacttc agatttgggg aaaccagtcg tatgagagta
     3481 aacatttctg gcgagttgca tagctttctg ccatacaagc aaatcccgat aatgacgagt
     3541 tctcgctcca gtttttggac tgactggctg acttgcttgc aaagcgctga cttgcttcat
     3601 gcgaactgct ccaggaaccg ggtatcccct gaatacaaca aaccaatgtc gctgatgccg
     3661 tacttcatca tcgcgatgcg gtctactccc atgccaaagg cgaagccgga gatcttcttc
     3721 gggtcgtagg ccggctgttt ttgctgcaca aatccgaaca ccgccgggtc aaccatgcca
     3781 cagcccaaca actcgatcca gccggagtgc ttgcatttgc ggcagccttt gcctccgcag
     3841 aaggggcaac tgatctgcac gtcggcgctt ggctccgtga acgggaagaa cgatgggaaa
     3901 aatcgcgtct tgacgctgga gccgaagaac tccttcatcg cgtggtcgag ggtgcccttc
     3961 aggtcagaga aggtgatgtt ggtgtccacg cagagaccct caatctggtg aaagatgggc
     4021 gagtgcgtgg cgtcggcctc gtcgttacgg tgcaccttgc ccggaatcac gatgcgcacc
     4081 ggaggcggct gctgctccat ggtgcggatc tgcaccggag acgtgtgcgt gcgcatcagc
     4141 aggcggtcgc gcagaggccg gcgttcctga ttagcgatga cgagcgtgtc ctgcgtgtcg
     4201 cgcgccggat ggttcggggg gaaattcagc gactcgaaat tgtagtagtc ggtttcgacc
     4261 tcggggccta cgccggctga gtatcccatg cgcttgaaga cggccaccat ctcgtgcatg
     4321 gtcttgatga gcggatgttc ggcgccgagg gcgcggcggg tccccgggag ggtgacgtca
     4381 atcgcttcgg catcgagcgc gaactgcgca ctggctccgg aggcccgctc caggcgctct
     4441 tcaatctgcg ccttgagctg gttaaagcgc tggccgaggg gctttttcgc ctccgcaggc
     4501 gatgatttga gccacgcatc gctgatgagt ttgaggcggc cctgtttgcg cccaagccat
     4561 tgcaggcgga actgctcgac gctttcgggt gagtggagcg cctgcgctga ggactcgacc
     4621 tcgcgcgcga gttcggcaaa
//
LOCUS       DASRBV010000406         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_252648, whole genome shotgun sequence.
ACCESSION   DASRBV010000406 DASRBV010000000
VERSION     DASRBV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252648"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1705
                     /gene="ggt"
                     /locus_tag="VFA02_14650"
     CDS             26..1705
                     /gene="ggt"
                     /locus_tag="VFA02_14650"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZU11142.1"
                     /translation="MQAQPVQTTQHETPVRMKHAMVVSIHHLATDAGVEVLKEGGNAV
                     DAAVATGFALAVVHPAAGNIGGGGFMLIHLHDGKSTFLDYREKAPLAASVNMYLDGKG
                     NVIPDMSIVGHKAIGVPGSVAGMTYAEKKYGKLTLARVMEPAIRLASEGFVLTEEEAQ
                     ELHESGLAEFSESRRIFQRDGNYYHAGEIFRQPELAATLRRIAKDPDDFYHGQMARRL
                     AADIQKGGGLITPKDLASYEVKEREPLTGTYKDYTIISAPPPSSGGVALLETLNILEG
                     YNLSRFGDRTPSEMHLITEAFRRAYMDRSDYLGDPDYVKIPIEQLLDKKYAAAWRASI
                     QEDQATPSASLKRPAGFLPPPPTMDDVRHESPETTHYSVMDTDGNAVAVTTTLNNSFG
                     SYVTATGLGFLLNDEMDDFASKQGAPNMFGLIQGPANSIAPGKRPLSSMTPTIVLKDG
                     KVAMVLGSPGGGRIITTVANIFLSVADQGLNIQEAVDAPRFHHQYLPDILYLEPGFPD
                     ATVNALRALGYQVKVSKGHWSDGECIAVDPQTGELEGGQDHRHHYGKAGGY"
     gene            complement(1776..>2487)
                     /locus_tag="VFA02_14655"
     CDS             complement(1776..>2487)
                     /locus_tag="VFA02_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU11143.1"
                     /translation="AVEKLITPRTKAIILNSPSNPTGSVIAPTDLERIVKLAHERGIY
                     LLLDECYVYLQYTGALVSGGSFTEAKEHVLILGSLSKTYAMTGWRAGYALGPKPIIAA
                     MSKLQSQSTSSTAHFVQKAAVAALNSSQECVVEMRADYLKLRDRILGRLAEIPGITCT
                     KPEGAFYVYPNVSAYLGKPGAQNALELANRLLHEAHVVTVPGEAFGTTQHIRLSYAVS
                     HDDIEEGLKRMKEFFAKL"
BASE COUNT          566 a          692 c          697 g          532 t
ORIGIN      
        1 ggttttctat ctttgttctt tttcgttgca ggcccagccc gtccagacga cgcagcatga
       61 aactcctgtc cggatgaagc acgcaatggt ggtcagcatc caccaccttg cgaccgacgc
      121 gggcgtggaa gttctgaaag aaggcggcaa tgccgtggat gctgctgtgg caacgggatt
      181 tgcgcttgca gtcgtccatc ctgcggcagg caacatcggc ggcggcggat tcatgctcat
      241 ccatcttcat gatggaaaat ctacttttct tgattaccgc gagaaggcac cgctcgccgc
      301 atcggtaaat atgtatctcg atggcaaagg taatgtcatc cccgatatga gcattgtggg
      361 acacaaagcc atcggtgttc ccggatctgt agcgggcatg acgtatgcgg aaaagaagta
      421 tggcaagctg acgctggcca gagtcatgga gcccgcgatt cgcctcgcat cggaaggctt
      481 tgtgcttacg gaagaagagg cgcaggagct gcatgaatcc gggcttgcgg agttttcgga
      541 atccaggcgc atcttccagc gcgacggtaa ttactaccat gccggcgaaa tcttcaggca
      601 accggaactt gccgccacgc tccgtcgcat tgccaaagac ccggacgatt tctaccacgg
      661 ccagatggca agacgacttg ccgcagacat ccagaaaggc ggcggcctga ttaccccaaa
      721 agaccttgcc tcctatgaag taaaagaacg cgagcctctc actgggacct acaaagatta
      781 cacaatcatc tctgctccgc ctccgtcttc aggcggagtt gccctgctgg aaactctgaa
      841 cattctggaa ggctacaatc tcagccgctt tggagaccgc acgccttctg aaatgcacct
      901 cattaccgaa gctttccggc gggcctacat ggaccgctcc gattatctcg gtgatccgga
      961 ttacgtgaaa attcccattg agcaactcct agataaaaaa tatgcagccg catggcgcgc
     1021 cagcattcag gaagaccagg ccactccgtc agcaagcctg aaacgacctg ccgggttcct
     1081 gccgcccccg ccgacaatgg acgatgtgcg ccatgagtca cctgaaacga cgcactactc
     1141 cgtcatggat actgatggca acgcagtcgc agttacgaca acgctgaata acagcttcgg
     1201 ttcttatgtc accgccactg gattaggttt tttgctcaat gacgaaatgg acgattttgc
     1261 ctcaaaacaa ggtgctccca atatgtttgg cctgattcag gggcctgcca actcgattgc
     1321 tccgggcaag cgcccgcttt cgagcatgac tccgaccatt gttctgaaag atggcaaagt
     1381 ggcaatggtg cttgggtcac ctggaggagg gcgcattatt actactgttg ccaatatctt
     1441 tctgagcgtt gctgaccagg gactgaatat tcaggaagct gtggatgcgc cccgctttca
     1501 tcatcaatac ctgccggaca ttctttatct ggaacctggg ttccccgatg ccacggtaaa
     1561 cgcattgcgg gcacttggat atcaggtaaa ggtcagcaaa ggacactgga gcgatggaga
     1621 gtgcatcgcc gtagatccac aaaccggaga actcgaagga gggcaggacc accggcatca
     1681 ttacgggaag gccgggggat actgaaccca attgcaaaaa gaaccagggc cagaggcttt
     1741 atgcccctgg cccgaaagat ctgaagctct tatctctaca attttgcgaa gaactccttc
     1801 atccgcttga gcccctcttc gatgtcatcg tgcgagaccg cataagacag gcggatgtgc
     1861 tgcgttgtac caaaggcctc gccgggaacg gtgaccacgt gggcctcatg tagcaggcgg
     1921 ttggcaagtt cgagagcgtt ttgcgcgcct ggtttgccca gatacgctga cacattcgga
     1981 tacacataga aggcgccttc gggtttggtg caggtgatgc cgggaatctc agccagacgc
     2041 cccagaatgc ggtcgcgcag cttgagataa tcggcgcgca tctcaactac gcactcctgc
     2101 gagctgttca acgccgccac ggcggccttc tgcacaaaat gcgctgtgga cgaggtggac
     2161 tggctttgca gcttgctcat cgcagcgatg atgggctttg gaccgagcgc gtagccggcg
     2221 cgccatccgg tcatggcata ggttttggag agcgagccga gaatcagcac gtgctctttc
     2281 gcttccgtaa acgagccgcc gctgaccagc gctccggtgt attgcagata gacgtagcac
     2341 tcgtccagca gcagatagat gccccgctcg tgcgccagct ttacaatgcg ctcaaggtcc
     2401 gttggggcga tgaccgagcc ggtcgggttc gagggcgagt tgaggatgat ggccttggtg
     2461 cgaggagtaa tcagcttctc gaccgct
//
LOCUS       DASRBV010000407         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_148440, whole genome shotgun sequence.
ACCESSION   DASRBV010000407 DASRBV010000000
VERSION     DASRBV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148440"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1434)
                     /locus_tag="VFA02_14660"
     CDS             complement(466..1434)
                     /locus_tag="VFA02_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU11144.1"
                     /translation="MARYLVTGCAGFIGSWIAQALVERGESVRGFDNFETGYKENLSG
                     LLDRIDFIQGDLRDADAVARACDGMDVIFHQGALPSVPRSVEDPLTSHTANIEGTFHV
                     LQAARAAKVRRVVYAASSSAYGNQDASPSVETLVPMPITPYAVQKVTGELYMRSYWQV
                     YGLETVSLRYFNIFGPRQDPSSPYSGVIAKFILLMLKGEQPTIFGDGEQGRDFTYVEN
                     AVSANLLAAAAPAEKVAGRVFNIACGQRHTLNKTYKTLAELTGFTQPPAYGPPRTGDI
                     QNSLADISAAREAFGYEPLVSFEEGLRRTVEWYKQQMKEPVYQHRD"
     gene            complement(1572..2513)
                     /locus_tag="VFA02_14665"
     CDS             complement(1572..2513)
                     /locus_tag="VFA02_14665"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZU11145.1"
                     /translation="MPIQPTSKIWHNGNLIPWEKAQIHVMSHVVHYGSSVFEGIRCYG
                     QPQGAAVFRLPEHMQRLIDSAKIYRMKLPYSLDQLCSATVELIEANSVAPCYIRPIAF
                     RGYGEVGVNPKNSPIEVYIANYPWGKYVGGDAGADVCISSWNRLAPNTMPSLAKAGAN
                     YMNSQLIRMEADTNGYAEGIALDSNGYVSEGSGENIFVVRNGVIYTPPLANSVLAGIT
                     RDSVLTLARHLGVPVVEQALPRELLYIADEVFFTGTAAEVTHIRSVDRIQVADGNIGP
                     ITKQLADEFFGIANGLKPDRFGWLTPVNVNAEQAVTV"
     gene            2728..>4682
                     /locus_tag="VFA02_14670"
     CDS             2728..>4682
                     /locus_tag="VFA02_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZU11146.1"
                     /translation="MRGFEETISQRLMFGVDYYPDQTPESLWEEDARGIAEAGLNTVR
                     IAEFAWALMEPSEGKFDFTWLHRAVDILHKRGIGVILGTPSAAPPPWLTEKYPEVFEV
                     NDHGMPLGPEGRRFTCPTNKTYRRLSVNMATEMAKSFASTPGVIGWQIDNELTLGSSG
                     RCYCKYCREGFQEWLRAKYGSLDKLNQAWGTAFWSQIYTDFAQVPVPLPSGAPPNPGM
                     ALDYDRYQSYANTSFLEEQLAVLRRLCPQHFITTNNVPLVDTLNQRELYANLDFVASD
                     NYPGFAAMYMEGATALQVVPSISFGHDYGRSIKDGRPFYIMEEQVGKAGQPTFSPQPE
                     KGQVRLWSYQAVAHGAMGVQYFRWDTATFGAEEYWHGMLNHDRSKSPAFDEIVQTVRE
                     LKALGPEALYASYASDVAIVFDNDADWAVSIQPGHPKLKYMNEVMTWYGAASAGHYGI
                     DVVDATRPLSRYKVVLAPFMYVVSEKQAENIRQFVRNGGVFITGYRLGVKDPASRIVT
                     TPLPGLLRDVMGVTVKEYVPVYSEKQKVKLAQTFGGAEGDCQLWYDVLAPEKADVLAT
                     YTSGRYAGDAAITSHSFGKGKAVYIGAGLDSASLGRVLNTLAASAGAKAELDAPYGVE
                     VTVRESGGKRWVYVLNHSGEQQTVPLK"
BASE COUNT         1011 a         1213 c         1435 g         1023 t
ORIGIN      
        1 ttgcgatgat gacggctggt cctgagcggc agaaaagaga gcaaccagca gcagcgctga
       61 agaaacgagc aaaggcttca acacagcggc ctccagagat gtaaggtgaa gtcgccgtga
      121 atagtacacc tcaagagaaa caaaggggaa gatgtttatt catccggtga aagaggattg
      181 cccgtttccg gacacaagat tccggaaatg ggcaccgcac gaggtgtcct ctttcggtcc
      241 cttcaactaa gtttctggag attcaacaac tttccatacg ccgcttttgg cgaggcaaat
      301 gccgtgtctt tctttaggtt gggaaaggcc gctatgagga acctttctca ggacattcgt
      361 tatgcgctgc ggcagttgcg gcgctctccc gggtatgtag ccacggttgt gctggctgtc
      421 acaatggcca ttggcgcaaa cgtgattgtg tttggcgtaa gtggttcaat cacggtgctg
      481 atagacgggt tccttcatct gctgtttgta ccactctacg gtgcgacgga ggccttcttc
      541 aaaactgacc aatggttcat agccaaaggc ttcgcgtgcc gcgctgatgt cggccagtga
      601 gttctggatg tctcctgtgc gcggggggcc gtatgctggc ggttgagtaa agccggtgag
      661 ttcagcgagg gttttgtagg tcttgttcag agtgtggcgc tggccgcagg cgatgttgaa
      721 gacgcgtccg gcaacttttt ctgcgggcgc ggctgcggcc agcaaattgg ccgaaactgc
      781 attttctacg taggtaaaat cgcggccctg ttcaccgtcg ccaaagatgg tgggctgctc
      841 gcctttgagc atcagcagaa tgaattttgc gatgactccg ctataggggg aactcgggtc
      901 ttgccgcggg ccaaagatgt tgaagtagcg caggctcacc gtctccagtc catagacctg
      961 ccagtaactg cgcatgtata gctcgcccgt tactttctgc acggcatacg gagtaatcgg
     1021 catgggaacg agtgtttcca cgctgggaga tgcgtcctgg tttccatagg ctgaagatga
     1081 cgctgcgtac acaacccgcc gcacctttgc tgcgcgcgca gcctgaagca catgaaatgt
     1141 gccttcgata tttgcagtat gactggtgag cggatcttct accgagcgcg ggacggaagg
     1201 caaagcgccc tgatggaaga tcacgtccat accgtcgcag gcgcgggcaa cagcgtctgc
     1261 atcacgcaaa tcgccctgaa tgaagtcaat tcgatcaaga aggccggaga ggttttcttt
     1321 gtagcctgtt tcaaaattgt caaagccacg gacagattct ccgcgttcga caagcgcctg
     1381 ggcaatccag gagcctataa atcctgcgca gccggtgact agatatctcg ccatattttc
     1441 tctatggtat tgcacccgac ggctcgcgat gagagcacag gccccaccct ttgctttcga
     1501 aaggatgggc cgcccatgta ctcacacaag cccagtagga ggccgtacgt atcgtccgga
     1561 atggatgctc tttatactgt tacagcctgt tcggcgttca catttacagg cgtgagccag
     1621 ccgaagcggt cgggcttaag gccgttcgcg atgccgaaga actcatcggc gagttgcttt
     1681 gtgatcggcc cgatattgcc atcggccacc tggatgcggt ccaccgagcg gatatgcgtg
     1741 acttcggcgg cggtcccggt aaagaagacc tcatcggcga tgtagagcaa ttcgcgtggc
     1801 agcgcctgct caactacggg gacaccgaga tggcgggcca gcgtcagcac agagtcgcgg
     1861 gtgattccgg cgagcacgga gttggccaga ggcggagtgt agatgactcc attgcgcaca
     1921 acgaagatgt tttcgcccga accttctgac acatagccat tgctgtcaag cgcgatgccc
     1981 tcagcgtagc cgttggtgtc ggcctccatg cggatgagct gcgagttcat gtaatttgct
     2041 ccggccttgg cgagcgaagg catggtgttc ggagcgaggc ggttccatga agagatgcag
     2101 acatcagcac cggcatctcc gccaacgtat ttcccccagg gatagttggc gatatagacc
     2161 tcaatgggag aattcttcgg gttaacaccg acctcgccat acccacgaaa cgcgatagga
     2221 cggatgtagc agggggccac gctgttggct tcaatcagct cgacagttgc ggagcagagc
     2281 tggtcaagcg agtagggcag cttcatgcga taaatcttgg ccgagtcaat caggcgctgc
     2341 atgtgctcgg gcagacggaa cacggccgcg ccctgcggct gtccatagca gcggatccct
     2401 tcaaagaccg atgagccgta gtggacgacg tggctcatga cgtggatctg ggccttttcc
     2461 cagggaataa gattgccgtt gtgccaaatc ttgctggtgg gctggatggg catggtgaag
     2521 ctgcgctcct ttttcttgtg aacctttgat tatagctgtg tcagacagca ttttgctcgg
     2581 gttttcacat gagagtggcg catcctttct gatttgggta caaaggtaca cacagaaagg
     2641 aaattccttc cgatggatag aagaaatttt ttaaaaagtg ccggagcagc cacagggttg
     2701 tggctggcgc ggcctctatc aggcgcaatg cgcggttttg aggaaacgat ttctcaacgg
     2761 ctgatgtttg gcgtggacta ctatcctgac cagacgcctg agtcactgtg ggaagaggac
     2821 gcgcgcggaa tcgccgaggc aggactgaac accgtgcgca tcgctgagtt tgcctgggca
     2881 ctgatggagc catcggaggg gaagttcgat ttcacctggc tccatcgcgc ggtagacatt
     2941 ctgcataagc gcggcattgg cgtgatcctg gggacacctt cggcggcgcc tccgccgtgg
     3001 ctgaccgaga agtatccgga ggtgtttgag gtcaatgatc acggaatgcc gcttgggccg
     3061 gagggaaggc gcttcacctg tccgacaaac aagacataca ggcggctttc ggtgaacatg
     3121 gccacggaga tggcaaaaag cttcgccagc actccgggcg tcattggctg gcagattgac
     3181 aatgagctga cgctcggcag ttctggccgc tgttattgca aatattgccg cgaagggttt
     3241 caagagtggc tgcgcgcgaa gtatggctcg ctcgacaaac tgaaccaggc ctggggcacg
     3301 gccttctgga gccagattta taccgacttc gcgcaggtcc cggtgccgct gccttccggt
     3361 gcgcctccga atccaggcat ggcgctcgat tacgaccgct atcaaagcta tgccaacacc
     3421 agtttcctcg aagagcagtt ggcagtgttg cgcaggctgt gtccacagca cttcattacg
     3481 acaaacaatg tgcctctggt ggacacgctc aatcagcgcg agctttacgc gaacctggac
     3541 tttgttgcct ccgacaatta ccccggattc gccgctatgt acatggaagg cgccacggcg
     3601 ctgcaagtgg tcccctccat ctctttcggt catgattatg ggcgcagcat caaggacggc
     3661 agaccgttct acatcatgga agagcaggtg ggcaaagcgg ggcagccgac cttctctccg
     3721 cagccggaga agggccaggt gcgtttgtgg tcttatcagg cagtggcgca tggagcgatg
     3781 ggcgtgcagt acttccggtg ggatacagcg accttcggcg ccgaagaata ctggcacgga
     3841 atgctgaacc acgaccgctc gaagagcccc gcttttgacg agattgtgca aacggtgcgc
     3901 gaattgaagg cgctggggcc ggaggcgctc tatgcatcgt atgcatcgga cgtggcaatc
     3961 gtctttgaca atgatgcaga ctgggcggtc agcattcagc cgggccatcc aaagctgaag
     4021 tacatgaacg aggtgatgac atggtatggg gcggcatccg ccggacatta cggcatagac
     4081 gtagtggatg cgacgcgtcc actttcccgt tataaagtcg tgctggctcc gttcatgtat
     4141 gtagtttcag aaaagcaggc ggaaaatatc cgccagttcg tgcgcaatgg aggcgtattt
     4201 atcaccggct accgtctcgg agtgaaggac ccggccagcc gcattgtgac cactccatta
     4261 cccgggctgc tccgcgatgt gatgggcgtc acagtgaagg agtatgtgcc ggtctattca
     4321 gaaaaacaga aagtgaagct tgcacagaca ttcggcgggg ctgagggaga ttgccagctc
     4381 tggtatgacg tccttgctcc ggaaaaggcc gacgtgcttg ccacgtacac atctggccgt
     4441 tatgcgggag atgcggccat caccagtcat tcatttggga aaggcaaggc tgtttacatc
     4501 ggcgcggggc ttgattctgc gagcctgggg cgggtgttga acacgctcgc tgcctcagcg
     4561 ggagcaaagg ccgagctgga tgctccgtat ggtgtggaag tcaccgtacg cgagtcgggt
     4621 gggaagcgtt gggtctacgt gctgaaccat tctggagagc agcagacggt gccattgaag
     4681 aa
//
LOCUS       DASRBV010000408         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_298395, whole genome shotgun sequence.
ACCESSION   DASRBV010000408 DASRBV010000000
VERSION     DASRBV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298395"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFA02_14675"
     CDS             <1..220
                     /locus_tag="VFA02_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU11147.1"
                     /translation="KQGKVGIRFKILPDGSVKEMVLELPSGDVALDRAAWGGITGASP
                     YPPLPKEFKGPFLELRFLFLYNIKPGEE"
     gene            235..516
                     /locus_tag="VFA02_14680"
     CDS             235..516
                     /locus_tag="VFA02_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11148.1"
                     /translation="MALRLVHLPRYRIWLSPFQQSIFCLFACAAGALTAFSLTAGRLF
                     ANLNTVPLSTEFRKRQIFGLLIIAAAVLVIALLRAPAHTIFPHGWWHVW"
     gene            498..1463
                     /gene="miaA"
                     /locus_tag="VFA02_14685"
     CDS             498..1463
                     /gene="miaA"
                     /locus_tag="VFA02_14685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZU11149.1"
                     /translation="MVARLVDPLLIVLPGPTASGKTSLSLHLAERYNGEVVSCDSVAV
                     YRDMEIGTAKPVKADRERLPHHLIDVVAPTEHYTAGDYSRDARRALKEIAARGHVPIV
                     TGGTGFYLRALLDGLFAGPQRSESLRERLKLSGKKHGRGWLYKVLMRLDPRSAERIHP
                     NDTPKLIRAIEVCIAARQPMSEAWKEGRDPLRGFRILRIGLEPDRERLYDRINKRAAA
                     MFRNGLVEETRMLLEKYGDARPFDSLGYKQAKALLRGEIREADAIALAQQGHRNYAKR
                     QLTWFRREPDIFWLHGFGDEGRAQRKVTEMVEEALAKEHSLRQGG"
     gene            complement(1443..2600)
                     /gene="rlmN"
                     /locus_tag="VFA02_14690"
     CDS             complement(1443..2600)
                     /gene="rlmN"
                     /locus_tag="VFA02_14690"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZU11150.1"
                     /translation="MPPGRKNSVSLILDAPAASATQTLFGLNHQQLTSLVQEFSVPEY
                     RARQLSDALYRQWVEDLDEVSTWPKSLRESLDAAGYRVGRPRIVEAFRSVDGTERYLI
                     ASSDGQTVETVWMPEGDDGEEGDGSEAQNDFHRATICVSSQIGCAVNCQFCLTAKLGI
                     VRNLTAGEIAGQVVAVLKRQKFEVGKQRINLVFMGMGEPFLNYDEFMAAVRLLVEEVG
                     ISESRMTVSTSGIVPGILRFAQEKTRPKLAISLNAPNDAIRSGIMPITRKWKINEVIE
                     AVRQVPLRPKEKITFEYVLLGGVNDRVSDADEVVRLARKANLPLKVNLIVWNPGPGMD
                     FHMSDAETVAKFQQRLRSSGIPAYIRRPRGRDIYAACGQLKRMTELTTLAQ"
     gene            2670..3944
                     /locus_tag="VFA02_14695"
     CDS             2670..3944
                     /locus_tag="VFA02_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZU11151.1"
                     /translation="MDNERNIQRTTLPNGLTILTEKMGHIRSVAMGVWIRTGSRHETP
                     EINGISHFVEHMVFKGTKSRSAQLIAREVDAIGGNLDAFTGKETVCFNIKVLDEHVPV
                     ALDILSDLVLNPVFATDEISRERGVILEEIKMDEDNPDYLVHEIFTQNFWKDHPLGRP
                     ILGTRETVRRFERDTLFDFYGQRFHGSNMIFSAAGNIDHDSFVEQVTRRFESLPAGTP
                     VTAQAPPKTAARIILKNKKALEQVQLCLGVPAPPIADESRYTTLVLNTILGGGMSSRL
                     FQTVREERGLAYSIYSDLNPYRDTGSLCVYAGTSADRALQVVQLVMEQFRRLKTEPLP
                     ADELRRAKDQLKGNLLLSLESSMSRMSNLARQQMYFDHFFGMQEILDRVEAVSEDQVM
                     AMANHLFQPEMVAVTLLGRLDGVKLNRSQLEC"
BASE COUNT          873 a         1324 c         1131 g          828 t
ORIGIN      
        1 caaacagggc aaagtcggca tccgctttaa gattctgccc gacggcagcg tgaaggagat
       61 ggtcctcgaa cttccttccg gagacgtggc gctcgaccgc gccgcctggg gcggtatcac
      121 cggagcttca ccctatccgc cgctgccgaa agagttcaaa gggccgttcc tggaactgcg
      181 cttcctcttc ctgtacaaca tcaagcccgg ggaagagtag cagtcagcga aaagatggct
      241 cttcgccttg tacatcttcc gcgttatcgc atctggctct ctccgtttca gcaatccatc
      301 ttttgccttt ttgcttgcgc cgcaggcgcg ctgaccgctt tttcgctgac tgctggccgc
      361 ctcttcgcta atctaaacac agtgcccctc tccaccgaat ttcgcaaacg ccagatcttc
      421 ggcctgctca ttatcgccgc ggccgtgctt gtaattgccc tgctgcgcgc gcctgcgcac
      481 accattttcc cgcacggatg gtggcacgtt tggtagaccc gctcctcatc gttctgccgg
      541 ggcctacggc cagcggcaag acgtcgctct cacttcatct cgccgaacgg tataacggag
      601 aagtcgtgag ctgcgattcc gtcgccgtct accgcgacat ggagatcggc acagccaagc
      661 ccgtcaaggc cgaccgtgag cgccttccgc accacttgat tgacgttgtc gcccccactg
      721 aacactacac cgcgggcgac tacagccgcg atgcgcgacg cgcgctgaaa gaaattgccg
      781 cgcgcggcca tgttcccatc gttaccggag ggactggctt ctaccttcgc gctctgctgg
      841 acggcctctt tgccggcccg cagcgctccg agtcgctgcg cgagcggctc aagctgagcg
      901 gtaaaaagca cgggcgcgga tggctctaca aagtcctcat gcggctcgac cccaggtccg
      961 ctgaacgtat ccatcccaac gacacgccaa agctgattcg cgccattgag gtctgcattg
     1021 ccgcgcgaca gcccatgtcg gaagcatgga aagaaggccg cgacccactg cgaggcttcc
     1081 gtatcctgcg catcggtctg gagccggacc gcgaacgcct gtacgaccgc atcaataaac
     1141 gcgccgcggc aatgttccgg aacggcctgg tcgaagagac ccgaatgctg ttggaaaaat
     1201 acggcgatgc acggccattc gattcgctgg gatacaaaca ggccaaagcg cttctgcgcg
     1261 gagaaattcg tgaagctgac gccattgccc tggcccagca gggccatcgc aactacgcta
     1321 agcgccagct gacatggttc cgccgcgagc ctgacatctt ctggctccac ggctttggcg
     1381 acgaggggcg agcgcaaaga aaagtcactg agatggtaga agaggccctc gctaaggagc
     1441 attcattgcg ccagggtggt taattctgtc atgcgcttca actgcccgca ggcggcgtag
     1501 atgtcgcgtc cgcgaggccg gcgaatatac gccggaatgc cactcgaccg gagccgctgt
     1561 tggaattttg cgaccgtctc tgcatcagac atatgaaagt ccatcccagg cccggggttc
     1621 cagacaatca gattcacctt aagcggaaga ttggccttcc gcgccagccg gacaacttcg
     1681 tccgcatcgg aaacgcgatc gttcactccg cccagcagca catattcgaa ggtgatcttt
     1741 tccttcggac gcagcggcac ctgccgcact gcttcaatca cctcgttgat cttccatttg
     1801 cgcgtgatgg gcatgattcc ggaacgaatt gcatcgttgg gcgcgttgag tgagatggcc
     1861 agcttgggcc tggtcttttc ctgcgcaaac cgcaaaattc ccggcacaat gcctgaagtc
     1921 gagaccgtca tccgcgactc ggagatcccg acttcctcga ccaggaggcg cacggccgcc
     1981 ataaactcgt catagttcag gaagggctcg cccattccca taaagaccag gttgatgcgc
     2041 tgcttcccaa cttcaaactt ctgccgcttc agcacggcaa ccacctggcc tgcgatttcc
     2101 ccagccgtaa gattgcggac gatacccagc ttcgccgtca aacagaactg gcaattcacg
     2161 gcgcagccaa tctggctgga gacgcagatg gtggcgcgat ggaaatcgtt ctgcgcctca
     2221 gacccgtcac cctcttcgcc atcgtcgcct tccggcatcc agacggtctc aaccgtctgg
     2281 ccgtcactgc tcgcaatcag atatcgctcc gttccatcta cagaccggaa cgcctccaca
     2341 atgcgcggcc gtccgacgcg atatccggcg gcgtcaaggg actcgcgcag cgattttggc
     2401 caggtggaca cctcgtccag atcctcgacc cactgccggt aaagggcgtc agaaagctgc
     2461 ctcgcccggt actccgggac cgaaaattct tgtactagcg acgtaagttg ttgatgatta
     2521 aggccaaaaa gagtctgggt cgcagaggcg gccggagcgt ctaaaataag agagacagaa
     2581 tttttcctgc ccggcggcat atagagacag cataacgcaa tctttcctca atctacctga
     2641 cgccccggca gcatccatgt aaaagcacga tggacaacga acggaacatc caaaggacga
     2701 ccctgcccaa tgggctgacg attcttaccg agaaaatggg ccacatccgg tctgtcgcca
     2761 tgggcgtgtg gatacgcacc gggtcgcggc atgaaacccc ggaaatcaac ggcatctccc
     2821 attttgtaga gcatatggtc ttcaaaggca ccaagtcgcg ctccgcgcaa ctcatcgccc
     2881 gcgaagtgga cgccattggc ggcaacctcg acgcctttac aggcaaggag accgtctgct
     2941 tcaacatcaa ggtcctggac gaacacgtcc ccgtagcgct ggacatcctt tccgacctgg
     3001 tgctgaaccc ggtctttgcc accgatgaaa tctcccgcga gcgcggggtc attctggaag
     3061 aaatcaagat ggacgaggac aatccggatt acctcgtgca cgaaattttt acccagaatt
     3121 tttggaagga ccatcctttg ggccgcccca ttctgggcac acgcgaaacg gtgcgccgct
     3181 ttgagcgcga cacgctcttt gacttttacg gacagcgctt ccacggcagc aacatgattt
     3241 tttccgccgc cggaaacatt gaccacgatt catttgtcga acaggtgacc cgccgcttcg
     3301 agtccctgcc tgccggaact ccggtaacgg cacaggcacc gccgaagacg gctgcgcgca
     3361 tcatcctcaa gaacaagaag gctctcgaac aggtgcagct ttgcctgggc gtgcccgctc
     3421 ctcccattgc cgacgagtcg cgctatacga ccctggtgct gaacacaatt cttggcggag
     3481 gcatgagttc ccgcctcttc cagaccgtgc gcgaagagcg cggactggca tactccattt
     3541 acagcgacct gaatccttac cgtgatacag gttcgctctg tgtctatgcc ggcacctcgg
     3601 cagaccgcgc cttgcaggtg gtccagttgg tcatggagca gttccggcgc ctgaagaccg
     3661 aaccgctgcc ggccgacgag ctgcgccgtg ccaaagacca gctcaagggc aacctcctgc
     3721 tctcgctcga aagctcgatg tcgcgcatgt ccaacctggc gcggcagcag atgtacttcg
     3781 accacttctt cgggatgcag gagatcctcg accgtgtgga ggccgtcagc gaggaccagg
     3841 tcatggcaat ggccaaccat ctcttccagc cggaaatggt ggccgtgacc ctgctcgggc
     3901 gtctcgatgg agtaaagctg aatcgcagtc agttggagtg ctgaaaacag ccgcagggcc
     3961 ggcttcgaag cattcgtata tcgagacccc gccattctga cgaccacccc atttccgtca
     4021 ttctgacgac cgaagggagg aagaatctca gcgatgcaag cttagccaat ccccgccttt
     4081 cccggcgtta aatcatccgg ccatccggtt ttgaaggtgc acggctttag ccgccggggg
     4141 atcgcctttc atctct
//
LOCUS       DASRBV010000409         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_43228,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000409 DASRBV010000000
VERSION     DASRBV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43228"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VFA02_14700"
     CDS             complement(<1..2045)
                     /locus_tag="VFA02_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HZU11152.1"
                     /translation="MTSRRSFFAFAFFALWISASASAQEPITARTPTTPLVLHDPYFS
                     VWSFDDALTDGPTRHWTGTPQQMAGLIRVDGKVYRFMGGERGDDGVPALKQVSRAIWP
                     TRTIYDFEDAGIHLTLTFFTPALPQDLDVLSRPVTYISWDVHSTDSQEHAVSLYFDAT
                     SQMATNTGEDTVVWGHALIGDMQTLRVGTNTQPVLEKSGDNLRIDWGWLYVAVPPQQG
                     VATSATNADARQQFAKDGALPKSDDLDMPRAVRHGLPLLAVRFDLGSVGSTPASRRLM
                     IAYDDRFSIEYLNRRLRAYWRRNGMTTAEMLEKAEADEPELRRKGEEFDRELTSDLER
                     EGGAHYAQLATLAYRQTIAAHKLVADVDGTPFLFSKENFSNGCIDTVDVTYPSSPFFL
                     LLNPKLLQAQLEPMMQYASLPRWKWPFAPHDLGTYPLANGQVYGGGERTEDDQMPVEE
                     SGNLIILTNALAHAVNSAAFAQKYWPLLTKWAEYLKEKGMDPENQLSTDDFAGHLPHN
                     TNLSIKAIEALGSYAQLAQMLGKTKEALAYRKLAQQMAAKWKDMAFDQDHYKLAFDQT
                     GTWSQKYNLVWDRILGLHLFSPQIIEQETAFYLKHQNTFGLPLDNRKTYTKLDWIVWT
                     ATLSGKQEDFVALTDPLYKYMTESPSRVPLSDWFETTDGKQVGFQARSVVGGVYIKML
                     "
     gene            complement(2142..>2429)
                     /locus_tag="VFA02_14705"
     CDS             complement(2142..>2429)
                     /locus_tag="VFA02_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HZU11153.1"
                     /translation="QTWGYDGRGIWVDRGCRADFFVGNSFGGGRGGNTVTCSSDNGGR
                     QYCPADTRGGVQLQKQRSGSPCIQGQTWGYDRNGIWVDRGCRADFVTGLSR"
BASE COUNT          552 a          714 c          618 g          545 t
ORIGIN      
        1 agcatcttga tatacacgcc accgactaca gaccgcgcct gaaatccaac ctgcttgcca
       61 tcagtggtct caaaccagtc gctcagggga acgcgtgatg gcgattcggt catgtattta
      121 tagagcgggt ccgtcaacgc aacgaagtcc tcctgcttgc cactgagggt tgctgtccag
      181 acgatccaat cgagcttggt gtaagttttg cgattatcga gcggcagccc aaaggtgttc
      241 tggtgcttca gatagaacgc cgtctcctgt tcgataatct gcggcgagaa cagatgcagc
      301 cccagaatac ggtcccacac aaggttatac ttctggctcc aggtaccagt ctgatcaaag
      361 gccagcttgt aatggtcctg atcgaaggcc atgtctttcc acttcgcggc catctgctgg
      421 gccaatttgc gataagcgag cgcctccttt gttttgccca acatctgcgc aagctgcgca
      481 tagctgccca aagcttcaat ggccttgatg gaaagattcg tgttgtgcgg caggtgtccg
      541 gcaaaatcat ctgtgctcaa ttgattttcc ggatccatgc ctttttcttt cagatactcg
      601 gcccattttg tcaacaacgg ccagtatttc tgtgcaaaag ctgcggaatt tacggcatgg
      661 gccagagcat tcgtcagaat aatcagattg ccgctctctt ctactggcat ctggtcgtcc
      721 tcggtgcgct cgccgccacc gtacacctgt ccattcgcca acggatacgt tcccagatca
      781 tgcggagcaa acggccattt ccagcgcgga aggctggcat actgcatcat cggctcgagc
      841 tgcgcctgca gcaactttgg attcaaaagt agaaaaaagg gcgatgatgg ataggtcacg
      901 tctacggtat caatgcagcc gttgctgaag ttttcttttg aaaaaaggaa gggcgtgccg
      961 tctacatcgg ccaccagctt gtgcgctgca atcgtctgcc gataggcaag cgtcgccagc
     1021 tgcgcatagt gcgctccgcc ttcgcgctca aggtctgatg tcaattctct gtcgaactcc
     1081 tcgccttttc tgcgcagctc cggctcgtcg gcctcggcct tttccagcat ttccgcggtg
     1141 gtcatcccgt tgcgacgcca gtatgcacgc aaacggcgat tcagatactc aatcgagaaa
     1201 cggtcatcat aagcgatcat caaccgccgc gacgccggag tgcttccaac actgcccaga
     1261 tcaaaccgca cagccagcag cggcaagcca tgtcttacag cgcgcggcat gtcgagatcg
     1321 tcgctcttcg gcaatgcccc gtcttttgcg aactgctgcc gcgcatcagc attggtggcg
     1381 gaagtcgcga ctccttgctg gggaggaaca gcaacataga gccatcccca gtcaatacga
     1441 agattatcgc cggacttctc cagaacaggc tgcgtattcg ttcccacacg cagggtctgc
     1501 atatcgccaa tcaatgcatg cccccacacg accgtgtcct cgccggtatt tgtagccatc
     1561 tgcgaagtcg catcgaagta gagcgaaact gcgtgctcct ggctgtctgt ggaatgcacg
     1621 tcccagctta tgtaagtaac cggtcgcgat agcacatcaa ggtcctgtgg taaggccggt
     1681 gtgaagaatg taagggtaag gtgaatacca gcgtcttcaa aatcgtagat ggtacgcgtc
     1741 ggccagatcg cgcgcgaaac ctgctttagc gcagggacgc catcatcgcc ccgctccccg
     1801 cccataaagc gatacacctt gccatccacg cggatgagtc cagccatctg ttgcggagtc
     1861 ccagtccagt gtcgcgtcgg accatcggtg agagcatcgt caaacgacca cacgctgaaa
     1921 taaggatcat gcagcaccag cggagtcgtc ggtgtgcggg ccgtgatggg ctcctgggct
     1981 gaagccgaag ctgaaatcca caaggcgaaa aaagcaaagg caaaaaacga gcgtcgtgaa
     2041 gtcattccct tttttattac aggatttctg atttcgtgta aacgctaaca ctgtgaaatg
     2101 gttcataaca accagacaaa ccagcgtacc ccgattgaaa gctacctact gaggccagtc
     2161 acaaaatctg cccggcaccc gcggtccacc cagatgccat tgcggtcgta tccccaggtt
     2221 tgcccctgaa tacagggaga gccactccgt tgtttttgta attgcacacc accgcgtgta
     2281 tctgcgggac aatactgccg tcctccgttg tctgaagagc aagtgactgt attgcctccc
     2341 cgccctccgc caaaactgtt tcccacaaag aaatcggccc tgcatccccg gtctacccag
     2401 atgccgcgtc catcgtaacc ccaggtctg
//
LOCUS       DASRBV010000410         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_150238, whole genome shotgun sequence.
ACCESSION   DASRBV010000410 DASRBV010000000
VERSION     DASRBV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150238"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFA02_14710"
     CDS             complement(<1..582)
                     /locus_tag="VFA02_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU11154.1"
                     /translation="MIRHPLGPRPKKLPLANRLFSNCIQAPAFFTATAFFGSLSLLAS
                     LFEKNGRMQHRIAQTWARVSVWISAAPVTIIGAEKLHRHKVAVYACNHLSYMDTPVIF
                     SSLPFQFRIVARHDLWKLPFIGWHLRRSGQVAVNVDNPRASISSLGSAVKTLKSGMPL
                     FIFPEGGRSESGHLQPFLNGPAFMAIRAQVPVVP"
     gene            complement(579..1889)
                     /locus_tag="VFA02_14715"
     CDS             complement(579..1889)
                     /locus_tag="VFA02_14715"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZU11155.1"
                     /translation="MSYAAAIESLFALAGELHSAPGQTRRKFDLEHMRVLAGALGHPE
                     RMFPSVLIAGTNGKGSTSATLASILHTAGLRTGLYTSPHLVRVNERVKINGRDISDDD
                     FARLYFYVDDVARKLVTEGRLPNHPSFFETMTAVAFTAFAEANLDMAVLEVGMGGRLD
                     ATNIVGPLVSVITDISLDHTEWLGPTISAISREKAGILRPNGVMVTLPQHPEANQVLG
                     EVATSLNVRGVNAAEYIPSRSTAGEYYPLQVMGETIEIEPPLAGQHQHRNIALAIATA
                     VELRNHHGYKITPADIAAGIRNTRWPGRMERFTSKNGAQVLLDVGHNPAGAWALRSVL
                     AEMSEQPRVLIFGCLRDKAVQEMAQILFPLFDRIFLTPVDSPRSASIEDLEAAAIATG
                     AVYEIMTDAHAALQRAQETVPSQGLIVGTGSVYLVGRLRAELTS"
     gene            complement(1913..2827)
                     /locus_tag="VFA02_14720"
     CDS             complement(1913..2827)
                     /locus_tag="VFA02_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZU11156.1"
                     /translation="MSISKLLAYAAIYILWGGSFLAIRVIVAEVPPFFAAGFRFTLAG
                     LLLIAYSHLRGEVACPKRALFNAAVLGFIMFTIMYAALFWGEIHVSSGIAAVVSAMIP
                     VWIFAGELTVLRTQKATVLSIAGILLGLTGVVVLAGGTRSTAGQTSGFALAALVGATI
                     CWSGGTLLSRRLTLPAPQTDSAGWQMASGGVLLLALSLVSGELRRLPASGILFSAKIL
                     MSMAYLVIAASILAYTAYVWLIAHEPATRVSSYAYVNPVIALILGTLLAGERLSAFQM
                     TGVALVLAGVVATLAAKRPVAEAKKVAA"
     gene            complement(3001..>4491)
                     /locus_tag="VFA02_14725"
     CDS             complement(3001..>4491)
                     /locus_tag="VFA02_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZU11157.1"
                     /translation="YSGIKTNEDHFHTVGLPDAAVRESRDRVRAAIKNSGFDVPSTHI
                     TINLAPADIRKEGSGFDLPMAIGILGAYGALQLRDLSQFLLVGELGLDGALRAVPGIL
                     PIAVAARSRGISNLVLPTANAREAGVVEGVNVYPVESLNQARELLNAAANGGIHTQPF
                     RMAATEMLGELQHFHLDYADVRGQQTAKRALEVAAAGGHNILMIGPPGSGKTMLAKRL
                     PSILAPLTFEEALETTKIHSVAGVLDAEAGLVTQRPFRAPHHTISDAGLIGGGAVPRP
                     GEVSLAHNGVLFLDELPEFPRNVLEVMRQPLEDRQVVIARASMSLTFPSAFMLAAAMN
                     PCPCGYFNDKSRECSCTPPMIQRYVSKVSGPLLDRIDIHIEVPAVQYRELRAGAASES
                     SSDIRARVLKAREIQHSRFSRAREKIYSNAQMTTRQIRAFCELGPDAERLLERAMQQQ
                     GLTARAHDRILKVARTIADLEGAASITVPHIAEAIQYRTLDRSYWS"
BASE COUNT          956 a         1258 c         1442 g          835 t
ORIGIN      
        1 gggaaccact gggacctggg cgcggatggc cataaatgcc ggcccgttca gaaaaggctg
       61 gaggtgcccg ctctcgctgc ggcctccttc aggaaagata aacagcggca ttcctgactt
      121 cagtgttttt accgcgctcc ccagactcga aatagaggcc cgcggattgt ccacattcac
      181 ggccacctgt ccggaccggc gcagatgcca gccgataaaa ggcagcttcc agagatcatg
      241 ccgagccacg atccgaaact gaaacggcag tgagctgaag atgaccggcg tgtccatgta
      301 agaaagatgg ttgcaggcat agaccgcgac tttgtgcctg tgcagctttt ccgccccgat
      361 gatggtcacg ggagcggcgc tgatccacac cgaaacccgc gcccaggtct gggcaatacg
      421 gtgctgcatg cgcccatttt tttcaaacag cgacgccagc agagacaggc tgccaaaaaa
      481 cgcagtcgcg gtaaagaacg caggggcctg gatgcaattg gagaaaaggc gattggccag
      541 aggcagcttc ttcggcctcg gccccagagg atgccggatc acgatgtcag ctccgcgcgt
      601 agtctgccga cgaggtagac cgaacctgtc cccacgatca acccctgcga tggaactgtt
      661 tcctgcgccc gttggagcgc agcgtgggca tccgtcatga tctcgtacac cgcgcctgtg
      721 gcaatggccg ctgcttccag gtcctctatg ctcgctgaac ggggtgaatc cacaggggtg
      781 aggaaaatgc ggtcaaagag aggaaagagt atttgcgcca tctcctgcac tgctttatcg
      841 cgcagacagc cgaagatgag cacgcgcggc tgctcactca tctctgccag aaccgaacgc
      901 agcgcccagg cacctgccgg attgtggccg acgtcgagga gaacctgcgc tccattttta
      961 gatgtaaatc tctccatgcg gccaggccat cgcgtattgc gaattcctgc agcgatgtct
     1021 gccggagtga ttttgtaacc atgatggtta cgtaattcta cggccgtagc aatggcaagc
     1081 gcgatattgc gatgctggtg ctgtccggcg agcggaggtt caatctcaat ggtttcgccc
     1141 atcacctgca atggataata ttcgcccgcg gtggagcgcg aaggaatgta ctccgccgca
     1201 tttacgccgc gaacgttgag cgatgtcgcc acttctccca gcacctggtt cgcttccgga
     1261 tgctgcggca gcgtgaccat gactccgttg ggacgcagaa tgccggcttt ctcgcgcgag
     1321 atggcgctga tggtcggccc gagccattcg gtgtggtcaa gcgaaatatc tgtgataaca
     1381 gagaccagcg gcccgacgat attggttgcg tccaggcgtc cgcccatccc gacttcgagc
     1441 acggccatgt ccagattcgc ttcggcgaaa gcagtaaacg cgacggccgt catggtctca
     1501 aaaaagcttg ggtggtttgg caggcggccc tctgttacca gcttgcgcgc cacatcgtct
     1561 acatagaaat agagccgcgc aaaatcgtca tcactgatgt ctctgccgtt gatcttcacg
     1621 cgctcgttca cgcgcaccag atgcggagag gtatagaggc cagtacgaag tcctgctgta
     1681 tgcagaatgg aggccagcgt ggcagaggtc gagccttttc cgttggtccc ggcaatcaga
     1741 acggagggaa acatcctctc cggatgtccg agcgcgccgg cgagcacgcg catgtgttcc
     1801 aggtcgaact tgcgccgggt ctggccgggc gcagaatgca actctcctgc aagcgcaaac
     1861 aggctttcaa tggccgcagc ataagacatg gtttgatttt agtggatgaa actcacgcag
     1921 cgaccttctt tgcttctgca accggccgtt tggcggcgag ggtggccaca actccggcaa
     1981 ggacaagcgc aacgcctgtc atctggaagg cggaaaggcg ctctccggcc agaagcgtgc
     2041 ccaggatgag cgcgatgacc ggattcacat aggcgtagct ggagacgcgg gtggcaggct
     2101 cgtgcgcaat cagccagaca taggcagtat aggcaagaat agaagcggcg atgacgagat
     2161 aggccatgct catcagtatc tttgcgctga ataaaatgcc gctggcgggc aggcggcgca
     2221 gttctcctga aaccagggac aaggccagca gaagcacgcc tccggaggcc atctgccatc
     2281 ctgcgctgtc ggtctgaggt gcaggaaggg tgagtctgcg cgacagcagt gttccgccgg
     2341 accagcagat ggtggcgccg acgagcgcag caagggcaaa gcctgaggtc tgtcctgcgg
     2401 tggagcgggt accgcctgcg agcaccacga ctccggtaag cccaagcaaa ataccggcga
     2461 tcgagagcac ggtggccttc tgtgtacgca ggacagttag ttctccggcg aaaatccaga
     2521 ccgggatcat ggccgacacg accgcggcga tgcccgagga gacgtgaatc tcgccccaga
     2581 agagcgccgc gtacatgatg gtgaacatga tgaagccgag cacggcggcg ttgaacaagg
     2641 cccgcttcgg acaggcgacc tctccgcgaa gatggctata ggcaatcagc agcaatccgg
     2701 cgagggtaaa gcgaaagccc gcggcgaaga agggagggac ttcggccaca atcacccgaa
     2761 tggcgagaaa tgatccgccc cagagaatgt agatggcagc atacgccagg agcttgctga
     2821 tagacatagc tcaggataac gcgagaagaa gagacagaaa aaatgtggag tcagcgaaaa
     2881 tatgctgtta ggaggcgtaa caagattcgc ggtgttggct gaccctcaca tcgctgacct
     2941 cgttccggtt gtcagaatga cgggccatcc atacacgaaa aaatggtata gagtgggccg
     3001 tcaactccag tagcttcggt cgagggtcct gtattggatg gcctcggcga tgtgcggcac
     3061 ggtaatggag gcggcccctt cgaggtcggc gatggtgcgg gccactttca ggatgcggtc
     3121 gtgggcgcgc gcggtgagtc cctgctgctg catggcgcgt tcaagcaggc gttcggcgtc
     3181 ggggcccagc tcgcagaagg cgcggatctg gcgcgtggtc atctgcgcgt tggagtagat
     3241 tttttccctg gccctggaaa aacggctgtg ctggatttcg cgcgctttca ggacccttgc
     3301 acgaatatcc gatgaactct cactggctgc tcctgcgcgc agttcgcggt actgaacggc
     3361 gggcacttcg atgtgaatgt caatgcggtc gagcagcggg ccggagacct tcgagacata
     3421 acgctggatc attggcggcg tgcaggagca ctcgcgggac ttgtcattga agtatccgca
     3481 ggggcacggg ttcatcgcgg ctgccaacat gaaggccgaa ggaaaggtca gcgacatgga
     3541 ggcgcgcgcg atgaccacct ggcggtcttc cagcggctgg cgcatcactt caagcacatt
     3601 gcgcgggaac tcgggcagct catcgagaaa gagcacgccg ttgtgcgcga gcgacacttc
     3661 gcccggacgc ggcactgccc ctccgccgat gagtccggca tcggagatgg tgtggtgtgg
     3721 cgcacggaat ggccgctggg tcacgagtcc ggcttcggca tcgagaactc cggcgacgga
     3781 atggatcttc gtggtttcga gcgcctcttc aaacgtcagt ggagcgagaa tcgaaggcag
     3841 tcgcttggcc agcatggtct tgccggagcc aggagggcct atcatcagga tgttgtgtcc
     3901 acctgcggct gcaacttcga gtgcgcgctt ggctgtctgc tggccgcgca cgtcagcgta
     3961 gtcgagatga aagtgctgca actcgccaag catttccgtc gccgccatgc ggaaaggctg
     4021 tgtgtgaatg cctccattgg cggcggcatt gagcagctcg cgcgcctggt tcagggactc
     4081 gaccgggtag acattcacgc cttcgacgac cccggcttcg cgcgcgttgg ccgtgggcaa
     4141 aaccaggttg gaaatgccac gtgagcgcgc ggccacggca atgggaagga tgccggggac
     4201 tgcgcgcaga gcgccatcaa gacccagttc gcccactagg agaaactgac tgaggtcgcg
     4261 caactggagc gcgccatacg ctccgagaat gccgatggcc ataggaaggt cgaagcccga
     4321 gccttccttt ctgatgtctg caggggcgag attgatggtg atgtgcgtgg aaggcacgtc
     4381 gaagccggag ttcttgatag cggcgcggac gcggtcgcgg ctctcgcgca cagcggcatc
     4441 ggggaggccg acggtgtgaa agtggtcttc gttggtcttg atgccggagt a
//
LOCUS       DASRBV010000411         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_401887, whole genome shotgun sequence.
ACCESSION   DASRBV010000411 DASRBV010000000
VERSION     DASRBV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401887"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFA02_14730"
     CDS             complement(<1..582)
                     /locus_tag="VFA02_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZU11158.1"
                     /translation="MSTAVAAKGLEGIVAANSGICWIDGDAGVLAYRGIDIHDLAEKS
                     TFEETTYLLWNGKLPTASELDAFKKKLSDARQLPPDIIRLLKEFPKTATPMEVLRTAV
                     SALSFYDENEKAVDHDSNLRKSFALTAQIAMIVAAFDRIRKGKPVVEADKSLSHAGNF
                     LWMLNGEKPSETATKAFDVALILHADHELNASTF"
     gene            complement(775..1821)
                     /gene="thiO"
                     /locus_tag="VFA02_14735"
     CDS             complement(775..1821)
                     /gene="thiO"
                     /locus_tag="VFA02_14735"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HZU11159.1"
                     /translation="MTASDVIIAGAGIIGLSTALELAAKGMKVTVLERQSAMSEASWA
                     AAGMLAAHDPENPPQLQPLSNLSISLYPEFLAKIEHLSGLRVPLRTTGTLQESPQSKD
                     TLSQNEIAGRVPALHSAGRQFIWLEEHSLDPRDLCAALPKAAKAAGVHIHENSPVTVV
                     HTTSTGVEAVTPNATYSAGHFVNCCGAWAGTIANHPAIGPRKGQMVVIKLPSNLRLDC
                     VIRTPELYLVPRGDDRIVIGASVEQAGFDKTVHETTIQELLRQAASLWPPVREAEIVE
                     TWAGLRPGSSDGLPLIDASGEQNCWVASGHFRNGILLAPGTARILRQLIFHEPLCIDL
                     SPFHCDRFTEAVVS"
     gene            1919..2743
                     /locus_tag="VFA02_14740"
     CDS             1919..2743
                     /locus_tag="VFA02_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11160.1"
                     /translation="MNEVSETGGEQRSFSGVFLGLLVLAVLAAAGGLIWSYLLNNRLA
                     RAEAQLLEAQQERDKIASDLRETNAKLKITSDSVGLTQKQLEARAQELMRRQESDAKR
                     LESEQAATKQQIGAVSSDVSSVKTDVGGVKTDVAQTKTELQSAEAQLQSMKGDLGIQS
                     GLIAKNHDELEVLKHKGDRNYYEFTLHKGQRQAVSTVGLELRKADTKHNRYTLNVYAD
                     DKKIEKKDRGLNEPVQFYTGKDNYLYELVVNNISKNEIQGYISTPKNAPVPVNPGH"
     gene            complement(2754..3311)
                     /locus_tag="VFA02_14745"
     CDS             complement(2754..3311)
                     /locus_tag="VFA02_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11161.1"
                     /translation="MDSTKRTLLIILAAIVIVVVVTGLYVYTGQKPPAATGEIVNLEI
                     FPVHSQLRVGAGAQGIQGGMETYDQLLVLARIKIHNQAKIPLFVRDVSANLDLNNGDS
                     QHSTAASRSDFQNVFTAYPQLASFKGEPLPWDLTLNPGQTVEGLAVFHYPITKDQWDA
                     RRSFHGVISFQYQQDLLLPWPPGKK"
     gene            3447..>4102
                     /locus_tag="VFA02_14750"
     CDS             3447..>4102
                     /locus_tag="VFA02_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11162.1"
                     /translation="MEQVLGVAIGIALVCLVLSIIASHVQEILASFVSSRARTLEIAI
                     LKMLDDPKLYEIFFEHPLIQNISFRPPKFLKLKFLSEDRPRPTYIPSELFSRVLLASL
                     VIRHDAVVTDFPGLVAIMPESSLKTKLQTLMTGILHDANACRLAVEQWYDNTMDRVNG
                     FYKRQTQWALFGLGLLLAILCNANLLHITTKLWDSQAARDQVNAVAQMYSCNGGPECE
                     A"
BASE COUNT          917 a          968 c         1271 g          946 t
ORIGIN      
        1 gaaggtggag gcgttcagtt cgtggtcggc gtggaggatg agggcaacgt cgaaggcctt
       61 cgttgcggtt tccgacggct tttcgccatt gagcatccag agaaaattgc ctgcgtgcga
      121 aagcgacttg tctgcctcga caacaggctt gcccttgcga atgcggtcaa atgcggcgac
      181 gatcatggca atctgcgcgg tgagtgcgaa gctcttgcgc aggtttgaat cgtggtctac
      241 ggccttttcg ttttcgtcat agaagctcaa cgcggaaacg gccgtgcgca gtacttccat
      301 cggggtcgcc gttttgggaa attctttgag gagcctgatg atgtctggag gaagctggcg
      361 ggcatcggag agttttttct taaaggcgtc gagttcggat gcggtaggaa gcttgccatt
      421 ccagaggaga taagtcgtct cctcaaatgt tgatttttcg gcaaggtcgt gaatgtcaat
      481 accgcggtag gcgagcaccc cggcatctcc atcaatccag caaatgccag agttggctgc
      541 aacaatccct tcgagtcctt tagctgctac tgcggttgac atgctgagta agattcctcc
      601 agtacagaat gaacattgtt tgatgtgctt cccgggcggg gggaagcagg aatggcgacc
      661 agaaaccaga aatcgcgcag gaagcccaac tattttttat atcagcgcag agattggatt
      721 gtcactttac gggcctgtgt tccgaatgct ttcagagctg ctgaaacatg attttcatga
      781 gacaacagct tcagtgaagc ggtcgcagtg aaaaggcgac aaatctatgc acagaggttc
      841 gtgaaaaatg agctggcgca ggatgcgcgc tgttccaggg gccagcagga tgccgttgcg
      901 gaagtgtccg ctggcgaccc agcagttttg ttcaccgctt gcgtctatga ggggaagtcc
      961 atcggaggag cctgggcgga gccctgccca ggtctcgaca atttcggctt cccggacagg
     1021 cggccacaga ctggctgcct ggcgcagaag ctcctggatg gtggtttcgt gtacggtttt
     1081 gtcaaaaccg gcttgttcga cgctggcgcc gatgacgatg cggtcatctc cgcgtggcac
     1141 aaggtagagt tccggcgtgc ggatgacgca atcgagacgc aggtttgaag gcagcttgat
     1201 gaccaccatc tggcctttgc ggggccctat cgcaggatgg ttggcaatgg tcccggccca
     1261 tgcgccgcaa cagttgacga agtgtccggc ggagtatgtt gcgtttggcg tgacggcttc
     1321 cactcctgtg gaggtggtgt ggaccacggt cacaggagaa ttttcatgga tatggactcc
     1381 tgcggccttc gctgcctttg gcagggcggc gcaaagatcg cgcggatcaa ggctgtgctc
     1441 ttccagccag ataaactgcc gcccagcaga atggagagcg gggacacggc ctgcaatttc
     1501 attctgcgaa agtgtgtctt tgctttgagg agattcctga agcgttccag tggtgcgcag
     1561 agggacacgg agtccggaaa ggtgctcgat ttttgccaga aactccggat ataaagagat
     1621 gctgaggttg gaaaggggct gcaattgcgg tggattttcc gggtcgtggg cggccagcat
     1681 tccggctgct gcccaggatg cctctgacat ggcgctttgc cgttccagca ctgtgacctt
     1741 catgcccttt gcggcaagtt ccagtgcagt ggaaagcccg atgatcccgg ctccggcaat
     1801 gatgacgtca gatgcggtca tggttctatt ttatggcgtt gtgaagtaaa aaatatgttt
     1861 gccaccatct atagagtcgc gtagtctcgg catacagttt tgaaaacaga ggggaaatat
     1921 gaacgaggtt tctgaaacag gcggggagca gcgcagcttc tccggagttt tcctgggact
     1981 gcttgtactt gcggtgctgg ccgcggccgg aggactgatc tggagctatt tgttgaacaa
     2041 caggctggcc agagcagaag cgcagctttt agaggcgcaa caggagcggg acaagattgc
     2101 gtcagatctg cgggagacaa atgcgaagct gaaaattaca tcggattcgg ttgggttgac
     2161 ccagaaacag ctcgaagcgc gtgcccagga actgatgcgg cgtcaggagt cagatgcaaa
     2221 acggctggaa agtgagcagg cggcaaccaa gcaacagatt ggcgccgtat ccagcgatgt
     2281 ttccagcgtg aagacggacg tgggtggcgt gaagaccgat gttgcacaga cgaagaccga
     2341 gctgcagagc gcagaagccc agctacagag catgaaggga gatctcggca tccagagcgg
     2401 actgattgcg aagaaccatg atgagctgga ggtcctgaag cacaaaggcg accgcaacta
     2461 ttacgagttc acattgcata aaggccagag gcaggccgtt tctactgtag gactggagct
     2521 tcgcaaggcc gatacgaagc ataatcgcta tactttgaac gtttatgctg acgataaaaa
     2581 gatcgagaag aaagaccgcg gactgaatga gccggtgcag ttctatacgg gcaaagacaa
     2641 ttatctctat gagctggtag tgaacaatat cagcaagaat gaaatacagg ggtatatttc
     2701 cacgcccaaa aatgccccgg tgccggtgaa tccggggcat tgagctccgg aagctatttc
     2761 tttcccggtg gccaggggag caggaggtct tgctggtatt ggaaagaaat gactccatgg
     2821 aagctgcggc gcgcgtccca ctggtctttc gtgattgggt agtgaaagac tgcgagtcct
     2881 tctaccgtct ggcctggatt cagggtaaga tcccagggca gcggttcgcc cttaaaggat
     2941 gcaagctgag gataggcggt gaagacattc tggaaatcgc ttcggctggc agccgtgctg
     3001 tgctggctgt ctccgttgtt caggtccaga ttggctgaaa cgtcgcgcac aaaaagcggg
     3061 atttttgcct gattgtggat tttgatccgg gccagcacaa ggagctggtc gtaggtttcc
     3121 atgccgccct ggattccctg ggcgcctgcg cccacgcgca actggctgtg aaccgggaat
     3181 atttcaaggt ttacgatttc gccagtggcg gccggaggct tttgccccgt gtacacataa
     3241 aggccggtga ccaccacaac aatgacgatg gcggcaagaa taatcagaag tgtacgcttg
     3301 gttgaatcca ctttctttgc gcctgtgacg cattctacga cattttcaca gattctatag
     3361 atttcatcgg gccgtgaaaa tgatgtgtcc ccgcaggcga tgggccgaca tatactggct
     3421 gcatttgatt gaggaggtct tgcgaaatgg agcaggtcct tggggtagcc attggtatcg
     3481 cgctggtttg tctagtgttg agcatcattg cttcgcacgt gcaggagata cttgcgtcct
     3541 ttgtgtcgag ccgcgcacgg acgctggaga ttgcaatcct gaagatgctg gatgatccga
     3601 agctttacga gatttttttt gagcatcctc tgattcagaa catttccttt cgtcctccga
     3661 aatttctgaa gctgaagttt ctttcagaag acaggccgcg gcccacgtac attccgtctg
     3721 agctgttcag cagggtgctg ctggcctcgc tggtgatccg gcacgatgct gtggtgaccg
     3781 atttcccggg gctggtggcc atcatgccgg agtcatcgct gaaaacaaag ctgcaaacgc
     3841 ttatgacggg aattctgcat gatgcgaatg cgtgcaggct ggctgttgag cagtggtatg
     3901 acaacacaat ggaccgtgtg aatggcttct ataagcggca gacgcagtgg gcgctgtttg
     3961 gactggggct gctgctagcg attctgtgca atgcgaacct gctgcatatt acaacaaagc
     4021 tctgggattc gcaggcggcg cgggaccaag tgaatgctgt tgctcagatg tatagctgca
     4081 acggaggtcc ggagtgcgag gc
//
LOCUS       DASRBV010000412         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936
            k141_300196, whole genome shotgun sequence.
ACCESSION   DASRBV010000412 DASRBV010000000
VERSION     DASRBV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300196"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFA02_14755"
     CDS             <1..390
                     /locus_tag="VFA02_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU11163.1"
                     /translation="TWDKEMQLMCPVNRLGHVPILIVSHHGWYQSSSPALVDAIHPQV
                     AIMDNGATKGGSKPTLETFQKDAGLETLWQLHYSNEGGADNTNEKYIANLQGPDTGND
                     IELTLTPAGALTVLNSRTGAKFSYTTK"
     gene            complement(446..823)
                     /locus_tag="VFA02_14760"
     CDS             complement(446..823)
                     /locus_tag="VFA02_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZU11164.1"
                     /translation="MILDTSALSALADGDVGLERSLRSTVQLAIPVIVLGEYRYGISQ
                     SRYRKQYEQWLSECLQKFRVLEVDEQTSAFYSAIRAELKKSGRPIPANDVWIAGLCRQ
                     HSLPLLSRDAHFDYVKGIRRVEW"
     gene            complement(820..1071)
                     /locus_tag="VFA02_14765"
     CDS             complement(820..1071)
                     /locus_tag="VFA02_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11165.1"
                     /translation="MKTTLEIPDSIFRRAKSAAAHRGIPFREYVTEAVKDKLTAEFRS
                     QEKPWTEVFGKLRHLRNETARINRMIAEEFEQIEAEDRL"
     gene            complement(1119..2426)
                     /locus_tag="VFA02_14770"
     CDS             complement(1119..2426)
                     /locus_tag="VFA02_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU11166.1"
                     /translation="MPADASLTTGTVPRRRWRIALLLAVGVLVNYLDRVNLSVSHNAL
                     YAAFGISDVMFGYLQSAYNWTYALCQLPIGVVLDKFGVRRVGRISTFLWSMASFAAAI
                     TPNIGGFFAARFLLGIGEAPTFPANAKATGYWFPPKERSFATALFDAQAKFSSAIGVP
                     LIGILLLHVGWRLSFAATGLISFCYFLLFYWIYRDPKDDPGLTQIERDYIANANAAEE
                     SDAAAEYASLAYLLRKKKVIGLALGFGSYNYVFYLLLTWLPSYLSRSLHIDLLQSFLY
                     TGVPWVFATITDLMIGGWLVDALVQRGWNADRVRRTVLISGTALGLGIFGAAYAHTPA
                     QALFWISLSIGGLAAAAPVGWSVPSLIAPRGSVGTLGGILNFSNQISGIAAPIVTGYL
                     VSAQHSFALAFGVAAAYLVAGILGYIFLLGKIEPIPGEHYLKT"
     gene            2489..>3232
                     /locus_tag="VFA02_14775"
     CDS             2489..>3232
                     /locus_tag="VFA02_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HZU11167.1"
                     /translation="MQSALQQFISQEATKAGFSAAGIASVPEPGSPEDLHERENFAQW
                     IEQGNAGEMEYLKRRNEAGDLVRSSLRVAVPWARSVIVCAATYHSAQPLSVHPAPEGS
                     GWIARYAWTGRPSGNGGLVPSDYHNVLLKRLEKLDIALKEHLGAFESKCYVDTGPLVE
                     RVYARYAGIGWTGKNTCILNQNLGSWIFLGVILTSLEVPEDHRATLAADRCGSCTRCI
                     DACPTGALTGPRQMDATRCISYLTIEKRGP"
BASE COUNT          814 a          975 c          785 g          658 t
ORIGIN      
        1 acctgggaca aggaaatgca gcttatgtgc cctgtgaaca ggcttggcca tgtccccatc
       61 ctcatcgtct cgcaccacgg atggtatcaa agctccagcc cagcgctggt agacgccatc
      121 catccgcagg tcgccatcat ggacaacggc gcaaccaaag gcggctctaa gccaaccctt
      181 gaaaccttcc aaaaagatgc cggcctcgaa acattatggc aactgcatta ctccaacgaa
      241 ggtggcgctg ataacacgaa cgagaaatac atcgccaatc ttcagggccc ggatactggc
      301 aatgacattg agcttaccct gactcccgca ggcgctctga ccgtgctgaa ttcgcgcaca
      361 ggcgcgaagt ttagttacac aaccaagtga gtgccagaac tagagtggcc ttcttcatac
      421 gtcatttcgc agaaggccaa caaaatcacc actccactct gcgaatacct tttacgtaat
      481 caaaatgagc gtcgcggctc aaaagcggga gcgaatgctg gcggcaaagt cctgcaatcc
      541 aaacatcatt cgccgggatg ggcctaccgg atttctttaa ctctgcgcga attgcgctat
      601 agaaggccga agtttgctcg tctacttcaa gaacacggaa tttctgcaaa cactcactta
      661 gccattgttc atactgcttg cggtagcgcg attgcgaaat tccgtaccgg tattcgccga
      721 ggacgatcac gggaatggcg agctgcaccg tgcttcgcaa agaacgctcc agccccacat
      781 cgccatcagc cagggctgag agggcactcg tatccagaat cacaaccggt cctcggcctc
      841 gatctgctca aattcttccg caatcatgcg attgattctt gctgtttcat tgcgaagatg
      901 gcgcagcttt ccaaaaactt ccgtccacgg tttctcctgc gacctgaact ctgcggtcaa
      961 cttgtccttt actgcttcag tgacgtattc acgaaaaggg atcccgcgat gggccgcggc
     1021 cgattttgcg cggcgaaaaa tgctgtccgg aatctccagt gtcgtcttca tagattcata
     1081 ctcccatatt tatgggatgc atctcaatgg gatgttcttt atgtttttaa ataatgctcg
     1141 ccgggaatcg gctcaatctt ccccaataga aagatatagc ccaggatacc ggcaaccaga
     1201 tacgcggcgg caactccaaa cgcaagcgca aacgagtgct gcgccgatac cagatatccc
     1261 gtcacaatcg gcgcggcaat gcctgaaatc tggttggaaa agttcaggat gccgcccaat
     1321 gtcccgacgc tgccgcgtgg cgcaatcaat gagggtacgg accagccaac cggcgcagcc
     1381 gccgccagcc cgccaatcga aaggctgatc cagaaaagtg cctgcgcagg cgtgtgggcg
     1441 tatgccgctc caaaaattcc cagtcccagt gcggtccccg atatcagtac cgtccggcgg
     1501 acccggtctg cattccatcc acgctggaca agcgcatcca ccagccatcc accgatcatc
     1561 agatcagtta tcgttgcaaa tacccacggc actccagtgt aaagaaacga ttgcagcaga
     1621 tcgatgtgca atgagcgcga aaggtaactt ggcagccacg tcagcaaaag ataaaagaca
     1681 tagttgtaag aaccaaatcc caacgccaga ccaatgactt tcttctttcg cagcagatac
     1741 gccaatgatg catactcagc cgcagcatcg ctttcctctg ccgcattcgc atttgcaatg
     1801 taatctcgtt cgatttgcgt caggccggga tcgtctttcg ggtcccggta aatccaatag
     1861 aacagcagaa aataacagaa actgatcagt ccggtagccg caaagctcaa cctccagccc
     1921 acatgcaaca gcaaaatgcc tatcagcggc actccgatgg ccgaagaaaa ttttgcctgc
     1981 gcatcaaaca gggccgtcgc aaagctgcgc tctttcggcg gaaaccagta acccgttgcc
     2041 tttgcgtttg ccggaaatgt aggcgcttca ccaataccaa gcaggaaccg cgcagcaaaa
     2101 aatcccccga tattcggtgt aatcgcagcc gcaaaggaag ccatgctcca gaggaatgtg
     2161 ctgatgcgcc ccacgcgccg cactccaaac ttgtccagca ccacgccgat aggaagctgg
     2221 cagagtgcgt aggtccagtt atacgcgctc tgcaaatagc caaacatgac atctgaaatt
     2281 ccaaacgcag cataaagcgc gttgtgcgat acagacagat tcacgcggtc gaggtaattc
     2341 accaacacgc ccactgcaag tagcagcgca atcctccagc ggcgtcgcgg aacagttcca
     2401 gtagtcaggc ttgcatctgc cggcaatgta ctttcgctcc tcaactactc cctcgtttct
     2461 caactaccat agagtagagc atttcttcat gcagtccgca cttcaacagt tcatttcgca
     2521 ggaagcaaca aaagccggct tttctgccgc cggaatcgcg tccgtgcccg agccaggctc
     2581 acctgaggac cttcacgagc gcgaaaactt cgcccaatgg atagaacaag gcaatgcagg
     2641 agaaatggag tatctcaaac gccgcaatga ggcaggcgat ctggtgcggt catctctgcg
     2701 cgtagctgtt ccttgggcca ggtccgtcat cgtctgcgca gccacctacc attctgcgca
     2761 accgctttcg gtccaccccg cgccggaagg ctccggatgg atcgcccgtt acgcatggac
     2821 agggcgccca tccggtaatg gcgggctggt tcccagtgac taccataacg tcctgctcaa
     2881 acggcttgaa aagctggaca tcgcgctcaa agagcacctc ggtgcattcg agtcaaaatg
     2941 ctatgtagat accggcccgc tggtggagcg cgtctacgcc cgctacgcgg gaataggatg
     3001 gacaggcaaa aacacctgca tcctaaacca gaacctcggt tcatggattt tcctcggagt
     3061 cattctcaca tcgcttgagg ttccggaaga tcatcgcgcc acacttgccg ccgaccgttg
     3121 tggaagctgc acccgctgca tagatgcctg ccctacaggc gctcttaccg gcccgcgtca
     3181 gatggacgcc acccgctgca tctcgtacct cacaattgaa aaacgcggac cc
//
LOCUS       DASRBV010000413         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_45554,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000413 DASRBV010000000
VERSION     DASRBV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45554"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFA02_14780"
     CDS             <1..753
                     /locus_tag="VFA02_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU11168.1"
                     /translation="LTITLHRPERRNALNPEMIQELTHALEAAEHSHIGAVVLTGAGT
                     AFCAGLDLDHLQTLHARTMEEHRVDSENIARVLRTLYDLSKPTIAAVNGAAIAGGMGL
                     ATVCDFTFAVPEAKFGYTEVKIGFIPAIVSAFLVRQVGDKVARDLLLSGRLFKADEAH
                     QLGLVNRVVSEQDLMPEARQLAQCLTKNSPEAMRATKRLLNQYTKERLDIELEAAISA
                     NVDARATEDFKEGIRAFLEKRKPEWPSKKITV"
     gene            complement(750..1376)
                     /locus_tag="VFA02_14785"
     CDS             complement(750..1376)
                     /locus_tag="VFA02_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU11169.1"
                     /translation="MNAVLFGATGMIGGRILQELLSRGHKVTAVVRNPEKVPANPAVK
                     AVSGDLLNTSQSAGLIQGADAVISAYSPGNEPAMIVDATRSLIAAAQQAGVKRVIMVG
                     GAGSLLVAPNLRLVDTPNFPAEWKPIATAHTDAFEVLQKSDLDWTYFSPAALIQPGKR
                     TGKFRLGTDNLITDAQGNSSISAEDYAIALVDELETPKHIRQRFTVGY"
     gene            complement(1523..2569)
                     /gene="ltaE"
                     /locus_tag="VFA02_14790"
     CDS             complement(1523..2569)
                     /gene="ltaE"
                     /locus_tag="VFA02_14790"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HZU11170.1"
                     /translation="MNEDRNMIDLRSDTVTRPTAAMRSAMAAAEVGDDVYGEDPTVNM
                     LEASAARIFGREAALFVPTGTMGNQIAIKVHTHPGQEIICESRAHIVDWEMAMAAAFS
                     GCQLRPVFAERGILTWKHIESVIKHPSTHNAPTGLIALENTHNMAGGTVTPLHVFGEI
                     WNGAREYGIPLHLDGARIFNASVALRVPVSELTSGFNTVMFCLSKGLCAPVGSMLVGS
                     RKTIERARMVRKALGGGMRQAGVLAAAGLIALEEMPKRLHQDHANARLIAEQIAGLPQ
                     IELDLATVQTNIVIFTPKTEGKASELVARLKQRGVLSGTVNRNSVRFVTHHDVDRAAC
                     ETAAKITAEEIAKL"
     gene            complement(2566..3222)
                     /gene="ccmA"
                     /locus_tag="VFA02_14795"
     CDS             complement(2566..3222)
                     /gene="ccmA"
                     /locus_tag="VFA02_14795"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZU11171.1"
                     /translation="MPTTVQLENVSRLFGTFAALRKISLTLDAGRCYVLLGENGAGKS
                     TLLRVMAGLLRPSYGTVRVLGQDPEQVRDRIGYMGHAPMLYDEYTAMENLQYFASLYR
                     TRTCLSGEEALRSVGLDPTLGRPIGQYSQGMRQRTSLARVLLSQPELLLLDEPFSNMD
                     IGSAYQMLALLEKIKGQQRTIVLTTHQRELAAPLADAMITLVAGSVDSIQSAGPAQKL
                     "
     gene            complement(3257..4099)
                     /locus_tag="VFA02_14800"
     CDS             complement(3257..4099)
                     /locus_tag="VFA02_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HZU11172.1"
                     /translation="MGLFRIAAINFLNPAPLMWDFEHGPRMRELAQRYVIHQTTPARC
                     ADELVQGTSDIGLVPVAAYASAPSWKIIPGCTIASLGDIRSIVLIVRHPDGVSGVRKV
                     ALDTSSRTSATYTRILFHRFWNREAKFIPHPPDLEAMLKVADAALLIGDPALFALEDR
                     EARRQRTGETLQYLDLGREWRKLTGSAWVSAFWAVRDIPVNAAVVVRDFQQSRDNGLA
                     HIEDLVREWSARMPVPASTIRAYLTSNIHYHLDEDCLEGLRLFYRYGAESGALPGLPE
                     LRFL"
     gene            4198..>5293
                     /locus_tag="VFA02_14805"
     CDS             4198..>5293
                     /locus_tag="VFA02_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU11173.1"
                     /translation="MLDRRTFMKFAAATATTTYLKPIFAQAQSPAPRSPNDNIHLALI
                     GAGIQGQFDTGVAVQVPGVKLVAVADCYDGRLTHAKELWGQDVFTTRDYREILTRSDV
                     DAVLIATPDHWHKQAAVDAMKAGKDVYLEKPMIHLYSDGPEIIETARKTNRILQVGSQ
                     RVSSVVYAKAKELLASGIIGKLNMVSAYWDRNSAIGAWDYSIPLDASEQTCDWPRFLG
                     TAPKIPWNAERFFQWRKWKDYGSGVAGDLFVHLFSGTHFITGANGPTRAMATGGLRYW
                     KDGRDAYDVLLALFDYPQEFNLALRVNFVDGGEESEGFLFTGSEGQMAIAGNTVTVTR
                     VPPATEPGYTVSTFADAMQQQYLEEYRKKYP"
BASE COUNT         1151 a         1434 c         1556 g         1152 t
ORIGIN      
        1 ctcaccatta ccctgcaccg acctgaacgg cgcaatgcac tgaatcctga gatgattcag
       61 gaactgacgc atgcgctgga ggccgcggaa cattcgcata ttggcgcggt ggtgcttacc
      121 ggggcgggta cggcattctg cgccgggctt gatcttgacc atctgcagac gcttcatgcc
      181 agaacgatgg aggagcaccg tgttgactcc gagaacattg cccgtgtgtt gcgcacgctg
      241 tatgacctga gcaagcccac aatcgcggca gtcaacggag cggccattgc aggaggcatg
      301 gggctggcca cggtctgcga tttcaccttt gctgttcctg aagcaaaatt tggttatacg
      361 gaagtgaaga ttggatttat tccagccatc gtttccgcat ttcttgtccg gcaggttggg
      421 gacaaggttg cccgcgacct gctgctctca ggccggttgt tcaaggccga tgaggcccat
      481 cagcttgggc tggtaaaccg ggttgtaagt gaacaggacc tgatgcccga agcgcgacaa
      541 ctggcgcagt gtctgacgaa gaacagtcct gaagccatgc gcgcgaccaa acgattgctc
      601 aaccaataca cgaaggaacg tctggatatc gaactggaag cagctatttc cgcgaatgtg
      661 gatgcgcgcg ccacggagga ctttaaagaa ggcattcgtg cctttctgga aaagcgaaag
      721 ccagagtggc cgagcaagaa gatcacagtt tagtatccga cagtaaagcg ttgacggata
      781 tgctttggcg tttcgagctc atctaccaaa gcaattgcat aatcttccgc ggagatgctg
      841 ctgtttccct gggcatcggt gatgaggttg tctgttccga ggcggaattt acccgtgcgt
      901 tttccgggct gaatcaaggc cgcgggacta aagtaggtcc agtcgagatc ggatttttgc
      961 agcacttcga aggcatcggt atgagcagtg gcgatgggct tccattcagc ggggaaattg
     1021 ggcgtatcta cgaggcgcag attcggagca accaaaaggc tgccggcgcc gcccaccatg
     1081 atgacgcgtt tcactccggc ctgctgtgcg gcggcgataa gcgagcgggt ggcatccacg
     1141 atcatggcag gctcgtttcc agggctatag gcgctgatga cagcatctgc tccctgtatg
     1201 agccctgcgc tttgcgaggt gtttagcaaa tcgccgctga cggcctttac ggcagggttt
     1261 gccggaactt tttccgggtt gcgaacgact gccgttacct tgtgtccacg agacaacaat
     1321 tcctgcagga tgcggccacc gatcattccg gtggcgccga ataagacggc attcataggg
     1381 ctactcctgt gcaagctatg tatgtgatgc tatgacgccg atgaaggttg aatttatttg
     1441 ttataacgga ttttattggc aagcgcctcg aaccaccaac gtccttcgtc ccttcggtcg
     1501 aatgctataa caccgggctt cgctaaagct ttgcaatctc ttctgccgtg atcttcgctg
     1561 ctgtttcaca ggcggcgcgg tccacatcgt gatgcgtaac aaagcggact gagtttctgt
     1621 tcacggtccc ggagagcaca ccgcgctgct tcagacgcgc tacgagttcg ctggccttcc
     1681 cttccgtctt cggggtaaag atgacaatgt tcgtctgcac tgtggccagg tcgagctcga
     1741 tctgcggcaa tccagcgatt tgttcggcga tgaggcgcgc gttagcgtgg tcctggtgca
     1801 ggcgtttggg catttcctca agcgcgataa ggccggccgc ggccagaaca cctgcctggc
     1861 gcatgcctcc gccgagcgct ttgcgcacca tgcgggcgcg ctcaatggtc ttccggctgc
     1921 ccacgagcat agagcctacc ggcgcgcaca gacctttcga caagcagaac attacagtgt
     1981 taaagccact ggtcagttca gaaaccggga cacgcagcgc aaccgaagca ttgaagatgc
     2041 gggccccatc cagatgcagc ggaatgccgt actcacgcgc gccgttccag atttcgccaa
     2101 agacgtggag gggcgtaacc gttccgccag ccatattgtg tgtattttca agagcaatca
     2161 ggccggtagg ggcattgtgc gtgctggggt gcttaatgac ggattcaatg tgtttccatg
     2221 tgagaatgcc gcgttcggca aagacgggac gcaattgaca gccggaaaat gctgccgcca
     2281 ttgccatttc ccagtccaca atatgcgcgc gtgactcgca gatgatttcc tgcccggggt
     2341 gtgtgtggac tttgatggct atctggttgc ccatcgttcc tgtaggaacg aagagcgcgg
     2401 cttcgcggcc aaagatgcgg gcggcgctgg cctccagcat gtttaccgta ggatcttcgc
     2461 cgtaaacgtc atcgccgact tcagcagcgg ccatggcgga acgcatggcc gcagtgggtc
     2521 tggttacggt atcgctgcga agatcaatca tgtttctgtc ttcattcata gcttttgcgc
     2581 aggccctgcg ctctgtatgg aatccacact accggccacc agtgtaatca tggcatctgc
     2641 cagaggtgcg gccagttcac gctggtgtgt tgtcaaaaca atcgtgcgct gctgtccttt
     2701 gattttttca agtaatgcga gcatctggta cgcgctgccg atgtccatat ttgagaacgg
     2761 ctcgtccaga agcaagagct cgggctgcga cagaagcacc cgcgccaggg atgtccgttg
     2821 ccgcatgccc tgcgaatact gcccgatggg gcgcccgagc gtgggatcga gtccaacgga
     2881 gcgtaatgct tcttctccgc tgaggcaggt ccgcgtgcga tacaggctgg caaagtattg
     2941 caggttttcc atggccgtgt attcgtcata gagcatgggc gcatggccca tgtatccgat
     3001 gcggtcgcgt acttgttccg ggtcctgacc taaaactctc accgttccat aagagggccg
     3061 aagcaagccg gccatcacgc gcaacagcgt agatttccct gccccgtttt cgcccagcag
     3121 aacatagcag cgtcctgcat cgagagtgag tgaaatcttt cgcaatgcgg caaatgttcc
     3181 aaagagcctg gagacattct caagctggac agttgtgggc atcggcagaa atcagtttac
     3241 aggggggaga tgtgaatcag aggaaccgca attcaggaag gcccggcagc gccccacttt
     3301 ctgcaccgta acggtaaaaa agccgaagcc cttcgagaca gtcttcatcg agatggtaat
     3361 gaatgttgct cgtaagatac gcccggatgg tcgaagccgg taccggcatt cgcgctgacc
     3421 attcgcgcac taaatcctcg atgtgcgcca ggccgttgtc gcgcgattgc tgaaagtcgc
     3481 gcacgacaac cgccgcattc accggaatat cgcgcacggc ccagaaggca gagacccagg
     3541 ctgatccggt cagtttgcgc cactcgcggc ccagatcgag atattgcaac gtctcgcctg
     3601 tgcgctgtct gcgggcttcg cggtcttcga gcgcaaacag tgccggatcg ccaataagga
     3661 gagcggcatc ggcgaccttc agcatggctt ccagatcggg cgggtgcgga atgaatttcg
     3721 cttccctgtt ccaaaacctg tgaaaaagaa tgcgagtgta tgtggccgat gtgcgcgagg
     3781 aagtatcaag ggcaactttg cggacaccag acactccgtc aggatgacga actatcaata
     3841 cgatcgaacg gatgtcgccg agtgacgcga tggtgcatcc tggaatgatc ttccatgaag
     3901 gagccgacgc atacgcggca accgggacca gcccaatgtc ggaagtgccc tgcaccagtt
     3961 catcggcaca gcgggcggga gtggtttgat gaattacata gcgctgtgcg agttcgcgca
     4021 tgcgcggtcc atgttcgaag tcccacatga gcggcgcagg attcagaaag ttgatggcag
     4081 caatgcgaaa aaggcccact taccagtgta gcaatgcgtc gcaaaatgga agcgtttcca
     4141 caaatcagat acaattgccg acgaatttaa ttttcgctta aaagtgaggc gttactgatg
     4201 cttgaccgca gaacgttcat gaagtttgct gccgcaactg ccaccacaac ctatctgaag
     4261 cccatctttg cgcaggcgca atcccctgct ccgcgctcgc caaatgacaa tatccatctg
     4321 gcgttgattg gagcgggcat tcagggccag tttgataccg gcgtcgcagt gcaggtgccg
     4381 ggagtgaaac tggttgccgt ggctgactgt tatgatggcc gccttacgca tgcgaaggag
     4441 ctctggggac aggatgtgtt cactacgcgc gactaccgcg agattcttac gcgctccgat
     4501 gtggacgctg ttctgattgc tactcccgac cattggcaca agcaggccgc ggtagacgcg
     4561 atgaaggcgg gcaaggacgt ttatctggaa aagcccatga ttcatctcta cagcgatggg
     4621 ccggagatca ttgaaacagc gcgcaagacc aaccgtatac tccaggtagg cagtcagcgt
     4681 gtaagttctg tggtctatgc aaaggcgaag gaactgcttg cttcgggtat tatcggcaag
     4741 ctgaatatgg tcagcgcata ctgggaccgc aactctgcga ttggcgcgtg ggactactcg
     4801 ataccgcttg acgcctcaga acaaacctgc gactggccgc gttttctggg tactgcgcct
     4861 aagattccct ggaacgcgga acgcttcttc caatggcgta agtggaagga ctacggctcc
     4921 ggggttgccg gcgacctgtt tgtgcatctt ttcagcggca cgcatttcat caccggggcg
     4981 aatggcccta cacgcgcgat ggccacaggc gggctgcgct actggaaaga tggccgagat
     5041 gcctacgacg tactgcttgc actgttcgat tatccgcagg aattcaacct ggccctgcgc
     5101 gtgaactttg tagacggcgg cgaagagagc gaaggctttc tctttaccgg atcggagggc
     5161 cagatggcga ttgccgggaa taccgttacg gtcacacgcg tccctccggc aaccgagccg
     5221 ggctacacgg tctcgacttt tgccgacgcg atgcaacagc aatatctgga ggaataccgg
     5281 aagaagtatc cgc
//
LOCUS       DASRBV010000414         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_45686,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000414 DASRBV010000000
VERSION     DASRBV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45686"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VFA02_14810"
     CDS             <1..1480
                     /locus_tag="VFA02_14810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZU11174.1"
                     /translation="TRIGPTSPEQTISVSVIVRRKNPLNLNELKGRRLSHEEFNAQYA
                     ADPSDFDRIRTFAQNHNLAVAEAASSLARRTIVLRGTASNMEKAFGVALHDYEGDKHK
                     KRFHCFEGAITLPEDISGIVEAVLGLDARPIAQPHFRKLDASAQATPQPLTAPQVAQL
                     YSFPANLDGTGQTIGILELGGGYTDSDLQTYFQGLGLNTPTVTAVSVDGATNSPGDPS
                     GADAEVELDIQVAGAVAPGASIAVYFAPNSDQGFIDAITTAVHDTANKPSTLSISWGG
                     PESSWSQSSMTALDNACQSAAALGVTITVASGDSGSSDGTSTNTVDFPASSPHVLSCG
                     GTELLANGNQISQEVVWNDQSSGGGATGGGFSATFSVPSWQTSAVPSGSTGRGVPDVA
                     ANAAPATGYNVVVDGQQETVGGTSAAAPLWAGLIALINQQLVQQGKQTAGFINPTLYQ
                     NGSDFHDITQGNNGAYSAGPGWDACTGLGSPNGTALLSALSQ"
     gene            complement(1643..2086)
                     /gene="rplK"
                     /locus_tag="VFA02_14815"
     CDS             complement(1643..2086)
                     /gene="rplK"
                     /locus_tag="VFA02_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZU11175.1"
                     /translation="MPPATGKKVQTQVKLQIEAGKATPAPPVGPALGQAQVNIMEFCK
                     QFNARTQNKEMAGLIIPVVITVYQDRSFTFVTKTPPASVLLKKAANLAKGSGTPNKNK
                     VGKVTEAQVLEIARQKMPDLNAASVEAAVKSIKGTARSMGIEVTA"
     gene            complement(2280..2882)
                     /gene="nusG"
                     /locus_tag="VFA02_14820"
     CDS             complement(2280..2882)
                     /gene="nusG"
                     /locus_tag="VFA02_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZU11176.1"
                     /translation="MAEDIQNGNTGGEAPGQLEPPANERFKWYIIHAYSGFERKVKES
                     IETRVQAFGLQNRIGRVMIPTEPVTEIVNGKKRTVERVFLPGYVLVEMDLDNDLWHVI
                     KNTPRVTGFLGTGDSPVALSEEEVSSILFRTEVSKEKPRLKVKFEKNESVRITEGPFA
                     NFNGIVDEVNEDRETLKVMVTIFGRSTPVELEFNKVEKVV"
     gene            complement(2902..3165)
                     /gene="secE"
                     /locus_tag="VFA02_14825"
     CDS             complement(2902..3165)
                     /gene="secE"
                     /locus_tag="VFA02_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZU11177.1"
                     /translation="MAKAAALATNEGGEPNRVTGYLTRATDFLKDVRNEMRKVVTPSR
                     SEVQSTTAVVIVTVFVFAAYFYVIDSVFGNALKWLLHWLGGTQ"
     gene            complement(3238..3313)
                     /locus_tag="VFA02_14830"
     tRNA            complement(3238..3313)
                     /locus_tag="VFA02_14830"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3277..3279),aa:Trp,seq:cca)
BASE COUNT          730 a         1022 c          842 g          744 t
ORIGIN      
        1 aacccgcatt ggccctacat cgccggaaca gacgatcagc gtctccgtca ttgtgcggcg
       61 aaagaaccca ttgaacctga acgaactcaa aggccgccgc ctctcgcacg aagaattcaa
      121 tgctcaatac gcagcagacc catcggactt tgatcgcatc cgcacctttg cgcagaatca
      181 caatcttgca gtagctgaag ctgcatcgag ccttgcacga aggacaattg tgctgcgcgg
      241 cacggcttcc aacatggaaa aggcctttgg cgtggccctg catgattatg agggtgacaa
      301 acacaagaag cggtttcatt gctttgaagg ggccattact cttcctgaag atatatcggg
      361 aatcgttgaa gcggtgctcg ggcttgacgc acgtcccatt gctcaaccac attttcggaa
      421 attggacgca agcgcgcagg ccactccgca accgttgact gcaccgcagg tggcgcagct
      481 ttacagtttc cctgccaatc tggacggtac aggccagacc atcggtattc ttgaactcgg
      541 cggcggctat acagacagcg acctgcaaac gtatttccag ggactcggcc tgaacactcc
      601 cacagtaact gctgtttccg tagatggcgc aaccaattct cctggtgacc caagcggcgc
      661 cgacgccgag gtcgaactgg atattcaggt ggccggggcc gttgctcctg gcgcaagtat
      721 cgctgtctat tttgctccca acagcgacca gggatttatt gacgccatca ccactgccgt
      781 ccacgacacg gcgaacaagc catctacact ttccatcagc tggggcgggc cggaatcaag
      841 ctggtcgcag tcttccatga ccgcgctcga caacgcctgc cagtccgccg ccgctctcgg
      901 cgtcaccatc accgttgcat ccggtgacag tggttcaagc gacggcacca gtaccaacac
      961 ggtggacttc cctgcatcca gcccgcatgt gctctcttgc ggcggaacag agctgctcgc
     1021 caacggcaac cagatttcgc aggaggtggt ctggaacgac cagtcctccg gcggaggcgc
     1081 aacaggaggc ggctttagcg ctaccttctc agtaccctca tggcagacca gcgccgtgcc
     1141 ttcaggaagc acaggacgcg gtgtccctga tgtcgccgca aatgcggccc ccgccaccgg
     1201 gtacaacgtt gtcgttgacg gccagcagga aactgtagga ggcaccagcg ctgctgctcc
     1261 gctgtgggcg ggactgattg cgctcatcaa tcagcagctt gtacaacaag gaaagcagac
     1321 cgcaggtttc ataaacccca cactctacca aaacgggagc gacttccacg acatcacaca
     1381 gggcaacaac ggggcctact ccgctggccc cggctgggac gcctgcaccg gactcggctc
     1441 tcccaatgga acagcgcttc tatcagcact gagtcaataa tccacagaaa gtgataacga
     1501 agagtgtctc tgcgaatcca gcacgagcgg ccatgaaatc gagcgcaaaa aagccaaacg
     1561 gcttccggaa actccggaag ccgcgactca cttccatgaa gcagacggaa tgacgaacat
     1621 tccgctttct cctttaaatt cgttacgccg taacttcaat gcccatagag cgcgccgtac
     1681 ctttgatgct cttcacggcc gcttcaactg aagctgcatt caagtcaggc attttctgcc
     1741 tggcgatctc gagaacctgg gcctcggtca ccttgccgac cttgttcttg ttcggagtac
     1801 ccgatccctt cgccaggttc gccgccttct tgagcagcac cgatgccgga ggcgtcttgg
     1861 taacgaacgt gaagctgcgg tcctgataca cggtgatgac gaccggaatg atgaggcccg
     1921 ccatctcctt gttctgagta cgcgcgttga actgcttgca gaactccata atgttgacct
     1981 gggcctggcc aagcgccggt cccaccggag gcgctggcgt cgctttcccc gcttcaatct
     2041 ggagctttac ttgtgtctgg actttctttc ctgttgctgg aggcatttct gaacctttct
     2101 acggcagcta ctagcatcta gctaataact tttagccaaa ttcacgtatt tgcagcagag
     2161 catctcaaga ggtagaaggc tctttgattt gcgtctgcta ttcgtgggcc aaagcccact
     2221 caatcagcag cccttaaact gaaagctggt agctaacagc cagaagctaa aagctgctct
     2281 taaaccacct tttccacctt gttgaactca agctcaacag gggtggagcg tccgaaaatc
     2341 gtcaccatca ccttgagggt ctcacggtct tcattgacct cgtccacaat gccattgaaa
     2401 ttggcaaatg gcccttcagt gatgcgtacc gactcgttct tctcaaactt gaccttaagc
     2461 cgcggcttct ctttcgaaac ctcggtgcgg aagaggatcg agctgacctc ttcttcgctc
     2521 aacgccacag ggctgtcacc cgttcccaga aagcccgtta cgcgcggcgt gttcttaatt
     2581 acgtgccaca ggtcgttgtc gaggtccatc tcgaccagca catatccggg caggaatacc
     2641 ctctcgacgg tgcgcttctt cccgttgaca atctcagtca ccggctcggt gggaatcatc
     2701 acgcggccaa ttctgttctg caaaccgaat gcctgcacac gcgtctcgat agattccttt
     2761 accttgcgtt caaagccgga gtacgcgtgg atgatgtacc acttgaaacg ctcgttggct
     2821 ggtggctcga gttgtccggg agcttcgcca ccggtatttc cattctggat atcttccgcc
     2881 atgttccttc tttcaacccg cctattgcgt gccacccagc cagtgcaaca gccacttcag
     2941 tgcgttgcca aaaaccgaat caatcacgta aaaatatgcc gcgaagacaa acaccgtcac
     3001 aatcaccacg gccgtagtgg actggacttc tgaacgcgag ggtgtcacca ctttgcgcat
     3061 ctcattgcgc acgtctttca gaaaatcagt ggcgcgcgtc aggtaacctg tcacgcgatt
     3121 tggttcgccg ccttcgtttg tggccaacgc agctgccttt gccatggact ttttcttttc
     3181 tgctacgggc atctcgccca acctctgcgg acacgccgca tctttctcat tcaaatctgg
     3241 caggggcgga gggattcgaa cccccaagtc cggttttgga gaccggcagt ttaaccgttg
     3301 agcttacgcc cctgttcgag ttgcctgctt gtcagtcc
//
LOCUS       DASRBV010000415         6795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_49678,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000415 DASRBV010000000
VERSION     DASRBV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49678"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFA02_14835"
     CDS             complement(<1..726)
                     /locus_tag="VFA02_14835"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU11178.1"
                     /translation="MQPFPDTMFFAVEITGKRRRLSFWQLQAGGWLAYFIVQLLSFLP
                     SKELREELLYKTVFCAICFFASFAQRIVCRRLWREQALWPRIVRRTAISCYLLGWVCG
                     TLLLWLDATYMMPSWEKFEWRATFANAISPAFVLALWSALYFIIKYYRAQSDERERLL
                     HMESLVRQAELKALQYQIHPHFLFNTLNSISTLVYEHDTATANRMIARLADFLRATLD
                     DDGAYEVPLRDELDITNLYLEIEK"
     gene            904..1956
                     /locus_tag="VFA02_14840"
     CDS             904..1956
                     /locus_tag="VFA02_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HZU11179.1"
                     /translation="MYTINRIACICMALFLGTSFSVAQTTSGPLTANTFSNPLLHTGP
                     DPWVVWWKGFYYYSNSTGTNLTLRKTADITDLEHAETKVVWTPGPGHPWSHDLWAPEL
                     HRWGRKWYIYFAADAGDNATHRIYVVENENDDPIKGTWNLKGQVKDPSDKWAIDATVF
                     QLRGQHYMVWSGWKGDTDGEQDLFIAHMSNPWTIDSPRTLISAPTYPWETHGDLPGRH
                     VSVNEGPEALVHDGKIFIVYSASGCWTDFYSLGVLEADIQADLLDARSWKKFDHPFLS
                     TDAEAHAFGPGHNGFFTSPDGRQNWIIYHANPGPDEGCGDLRSPRIQPFTWNPDGSPN
                     FGKPVPLGRPLEKPAR"
     gene            complement(1960..2469)
                     /gene="def"
                     /locus_tag="VFA02_14845"
     CDS             complement(1960..2469)
                     /gene="def"
                     /locus_tag="VFA02_14845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZU11180.1"
                     /translation="MIREIVKYPDPVLQRPTEPVTEFNDELRKLVDDMFESMYAAQGI
                     GLAAPQIGVSKRLTVIDLSFQKDPKEKIVLINPEIIHKEGRQVEEEGCLSLPEIREKV
                     SRAHKVRVRAQDVEGKWFEIDGEELLARAFQHEIDHLDGVLFIFRVSALKRDLLLRKI
                     RKMQRAGEW"
     gene            complement(2502..3683)
                     /gene="aroC"
                     /locus_tag="VFA02_14850"
     CDS             complement(2502..3683)
                     /gene="aroC"
                     /locus_tag="VFA02_14850"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZU11181.1"
                     /translation="MLRFSTAGESHGESLIALVSGLPAGIEVDLSFINRELWRRQQGY
                     GRGGRMRIEQDTAHILSGVRHGKTIGSPVAIEIENRDWKNWTEVLPVEKGDPAKHKAV
                     ASPRPGHADLAGALKYDFPDARYVLERASARESTARVAAGALAKLLLLSLGIEIGSHV
                     IRVGHAELERDAAWEEIAALRERDEVLLGCVDAEAEARMKEEVDKVLRTGDTVGGVFE
                     VVIHNAPAGIGTHANWDERLDGILAQAVMSLQAVKAVEIGRGVTAAESFGSEVHDAIG
                     YASELPDGPRTRFTRERNNAGGVEGGISNGEDIIVRGYLKPISTLRRPLQSVRFDTRE
                     TTKAAYERSDVCVVPAAGVAAEAMVALAFARLVLEKFGGDSLRELKRNYDGYVEQIRA
                     F"
     gene            4179..5009
                     /locus_tag="VFA02_14855"
     CDS             4179..5009
                     /locus_tag="VFA02_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11182.1"
                     /translation="MKYLPLFAAAFFTFGIHAQTASTAAGTQSTVQTAPSSQVSGSTA
                     SQANAVVEGQKASAAQATNLSAELTRKVDTKNAKVGDEVVARTTSAAQLSDGTKLPKG
                     TRLLGKVTDVQAKSAEQKESRLAFTFDRAVLRSGRTLPIHATVMSLSAPVSAAAMAEN
                     DDSMSAGPAPAPVATSAGGRASGGGLLGGGALRGAGSALGGVTSTAGNTLHTTTALGS
                     QAVHQTTDTVREQVTNMPGVNFTSEAGASNSAELNAQGKNISLVTGTQMMLNVSASKQ
                     "
     gene            complement(5072..6196)
                     /gene="hppD"
                     /locus_tag="VFA02_14860"
     CDS             complement(5072..6196)
                     /gene="hppD"
                     /locus_tag="VFA02_14860"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZU11183.1"
                     /translation="MSTLAHPEVQTQKDFLPLNGTDYVEFYVGNARQAAYYYRLAFGM
                     RLVAYAGPETGVRDRASYVLQQGKIRFVLTTPLRPDGEIAEHVHRHGDGVHDIALWVD
                     DAKYAWKETTKRGARSIREPFELRDEHGVVRLSTIATYGDTVHTFVERSEYKGVFMPG
                     YRPVEEDKLARPVGLLHVDHMVGNVGWNEMNRWVDFYADVMGFSLYQHFDDKDISTEY
                     SALMSKVMANGNGRVKFPINEPAEGRRKSQVEEYLEFYHGPGVQHIAMATNDILHTVS
                     QLQAQGVEFLKVPHTYYTELEKRVGKIDEPIQELEKLGILADRDDEGYMLQIFTRPVE
                     DRPTLFYEIIQRKGSRSFGKGNFKALFEAIEREQAARGNL"
     gene            6311..>6795
                     /gene="pncA"
                     /locus_tag="VFA02_14865"
     CDS             6311..>6795
                     /gene="pncA"
                     /locus_tag="VFA02_14865"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HZU11184.1"
                     /translation="MSFKTAGHDVLLIIDVQNDFCPGGALAVPHGDEIVPVINQLAQQ
                     FAHVILTQDWHPKGHLSFASRHPGKSPFDVVEVSYGPQTLWPDHCIQASRGAEFHPDL
                     KVPHAELVLRKGFRPEIDSYSAFQENDRKTKTGLEGYLRERGFQRVFLAGLAYDYCVR
                     YS"
BASE COUNT         1577 a         1965 c         1735 g         1518 t
ORIGIN      
        1 cttttcaatc tccagataaa gattggtaat gtccaactca tcacgcaatg ggacttcgta
       61 ggcgccatca tcatcgagtg tagcgcgcag gaaatccgca agccgtgcaa tcatgcggtt
      121 cgctgttgcc gtgtcatgct cgtagaccag ggtggaaatc gagttcagcg tgttgaagag
      181 aaaatgcgga tggatttgat attgcagggc ctttaattcc gcctgccgca ccagcgattc
      241 catgtgcaac agccgctctc gctcatcgct ttgcgcgcgg taatacttga tgatgaagta
      301 gagcgcgctc cagagcgcca gcacaaatgc aggactgatg gcgttggcga atgtcgccct
      361 ccattcaaac ttctcccacg atggcatcat gtacgtcgcg tccagccaca acaggagcgt
      421 tccgcagacc catcccagca ggtagcaact gatcgcagtc cttctgacga tccgcggcca
      481 tagtgcctgt tcgcgccaca aacgcctgca aacaatgcgc tgtgcaaagc tggcaaaaaa
      541 gcagatggcg caaaagaccg ttttatagag cagctcttcg cgcagttctt tcgacggcaa
      601 aaagctcagt agctggacaa tgaaataggc cagccagcct ccggcctgga gctgccagaa
      661 ggagagtcgc cgccgtttgc cagtaatttc cacagcaaaa aacatggtat ccggaaaagg
      721 ctgcataagc atatttccgt cagtgccgcg cgacgcaaaa tttgcacggt tgaacgcgac
      781 gcaagcgttt gcattgtttc ctgcaccctg aaatggcacg aaaagatgaa tcgtgcgaaa
      841 agaacattga ccctgaaggc ataaaatctt tcccaggtgc atcttttgcc ctataagacc
      901 gccatgtaca cgatcaaccg tattgcctgc atctgcatgg cactgttttt gggcacatca
      961 ttcagcgttg cccagaccac atccggcccc ctcaccgcca acacatttag caatcctctg
     1021 ctccataccg gtcctgatcc gtgggtggtc tggtggaagg gcttttacta ttactcgaat
     1081 tccacaggca ccaatctgac tcttcgcaaa acggccgaca tcacagatct tgaacatgca
     1141 gagacgaaag tggtttggac acctggtcct ggacatccgt ggtcgcatga tctgtgggcg
     1201 cctgagcttc atcgctgggg caggaaatgg tatatctact tcgccgccga tgctggcgac
     1261 aatgccaccc accggatcta cgtcgtcgag aacgagaatg acgatccgat caagggcacc
     1321 tggaacctca aaggccaggt aaaagaccca agtgacaagt gggccattga tgccacagta
     1381 ttccaactgc gtggacagca ctatatggtc tggtcgggct ggaaaggcga tacggacggc
     1441 gaacaggacc tctttattgc ccacatgagc aacccctgga cgattgactc gccgcgaacg
     1501 ctgatttcag ccccaaccta cccctgggaa acgcacggcg atctaccggg caggcacgta
     1561 agcgtaaatg aaggcccgga agcattggtg catgatggaa aaatctttat tgtctattcg
     1621 gcatccggct gctggacgga cttctacagt ctgggtgtgc tggaggcaga cattcaggct
     1681 gacctgctcg atgcccgaag ctggaagaaa ttcgatcatc ctttcctgag cacagatgca
     1741 gaagcccatg cctttggacc ggggcacaac ggcttcttca cttcccccga tggcaggcag
     1801 aactggatca tctatcacgc caatcccgga cctgacgaag gctgcgggga ccttcggtcg
     1861 cctcgcattc agcctttcac ctggaaccct gatggttctc cgaacttcgg caaacccgtt
     1921 cccctgggca gaccgcttga aaaacctgcg cgctaaactc taccactcac ccgcacgctg
     1981 cattttgcga attttgcgca gcagcaggtc ccgcttcagc gcgctgacac gaaaaatgaa
     2041 aaggacccca tcaaggtggt caatttcgtg ctggaaggcc cgggccagaa gctcttcccc
     2101 gtctatctca aaccactttc cttcaacgtc ctgtgcgcgc acgcggactt tatgggcacg
     2161 ggagaccttt tcgcggattt ccggaagact caggcaaccc tcttcttcca cctgcctgcc
     2221 ttctttgtgg atgatttccg gattaataag gacgattttt tctttgggat ctttctggaa
     2281 actcagatca atgactgtta gccgctttga cacgccaatc tggggcgcgg ccaggccgat
     2341 tccctgagcg gcatacatgg attcaaacat gtcgtccaca agtttccgga gctcatcgtt
     2401 gaactcggtg acgggttccg tgggacgctg aagaaccggg tctggatatt tgacgatttc
     2461 tcggatcatt tacttctgtt cgatttatgg tttggccctg ttcaaaaagc gcgaatctgc
     2521 tccacgtaac catcatagtt acgtttcaac tcgcgcagtg agtcgccgcc gaatttctcc
     2581 agcaccagcc gggcaaaggc cagcgccacc atggcctctg cggcaactcc cgccgccggg
     2641 acaacacaca catcgctgcg ttcgtaggcc gcctttgtgg tctcccgcgt gtcgaagcgc
     2701 actgattgca gtgggcggcg caaggttgaa atcggtttta aatatccacg cacgataatg
     2761 tcttctccgt tggagatgcc gccttccaca ccgccggcat tgtttcgctc gcgagtaaag
     2821 cgcgtgcgcg ggccatctgg cagttcactc gcgtatccaa tagcatcgtg cacctcggag
     2881 ccgaaggact ccgccgctgt aacacctcgc ccaatttcga ctgctttgac tgcctgcaga
     2941 gacatgacag cctgggcaag aattccgtcc aggcgctcgt cccaattcgc atgggtgccg
     3001 atacccgcgg gcgcgttgtg aatcactacc tcaaagaccc cgcccacggt atcacccgtg
     3061 cgcaggacct tgtctacttc ttctttcatg cgcgcctcgg cttccgcatc cacgcagccc
     3121 agcagcactt cgtccctctc ccgcaaagcc gcgatttctt cccaagcggc gtcgcgttca
     3181 agttcggcat gacccacgcg gatgacgtgg cttccaatct ctattcccag cgaaagcaga
     3241 agcagcttcg ccagggcccc ggcagccacg cgcgcagtag attcacgggc cgaagcgcgt
     3301 tccagcacat aacgggcatc aggaaaatcg tacttgagag caccggcaag gtcggcgtga
     3361 cccgggcgcg gcgaggcgac agctttgtgc ttcgcagggt cgcccttctc taccggcagc
     3421 acttcagtcc agtttttcca gtcacggttt tcgatttcga tagcaacggg cgagccgatg
     3481 gtcttgccgt ggcgcactcc ggagagaata tgtgccgtgt cctgctcaat gcgcatgcgt
     3541 ccgccgcggc catagccctg ctggcgccgc cataactcac ggttgataaa acttaagtca
     3601 acttcaatac cggccggaag cccggaaacc aaagcaatca gactctcgcc gtgcgactca
     3661 cctgcggtag aaaaacgtaa catttgcgtg atttaccaat tttgattgta gcgcagcagc
     3721 catctcactt tctttctgca tctcgaacgg cccaggacca tggatacagg cgatacggca
     3781 cttttgttcc tgtcctgatg cttcgccggg acgatccggg gaaacattga tgacctccac
     3841 aatttccgcg ccttgcagaa catactttat tggaagttct tatctctccc tgacgctgtt
     3901 cttacggagt ggagcatttt cgaagcgcga cactctttaa cggaaacaga tagatgtctt
     3961 tctggtaaac acgaagaatt tactcaatga aatgagaaat ctgcgcatct gtgcatccag
     4021 gcacctcttt tcccgcggaa gaaagccggc ttctaaatac tttaattttt atagatttat
     4081 agcatcggac atctttatgg ctttttgttt gcatctgtga gaaacgcaac tgatgcgaga
     4141 cttgcgtgtt cagttgaaat gcaagtgagg aaaggcccat gaaatatctt ccgttgtttg
     4201 cagcagcatt tttcacgttc ggcatacatg cacaaacggc ttctaccgcg gcaggcactc
     4261 agagcaccgt acaaaccgcg ccttccagcc aggtgagtgg ctcaacagcg tcacaggcga
     4321 atgccgtggt cgagggacag aaggcatctg ccgctcaggc cacaaatctg tccgccgagc
     4381 tgaccaggaa agtagatacg aaaaacgcaa aagtcggcga cgaagtagtt gcgcggacca
     4441 ccagtgcagc acaactgagc gacgggacaa aattgcctaa gggaacacgc ctgttaggaa
     4501 aagtgacgga tgttcaggcg aagtcggcgg aacagaaaga atcacggctg gcatttacat
     4561 ttgatcgcgc tgtgctgcga agcggccgca cgctgccaat ccacgctact gtgatgtcgt
     4621 tgagcgctcc tgtatcagcc gcagccatgg cggaaaatga tgattccatg agtgccggcc
     4681 ctgcgcccgc tcctgtcgca acatcagcag gtggacgcgc ttcgggcggt ggtcttctgg
     4741 gcggaggcgc actgcgtgga gccgggagcg cgctaggtgg cgtaacatcc actgcaggca
     4801 atactctgca cacaacgaca gctttaggat cacaggctgt acatcagacc accgataccg
     4861 tcagggaaca ggtgacaaac atgcctggag tcaatttcac ctctgaagcc ggcgccagca
     4921 acagcgcaga gctgaatgct cagggaaaga acatttctct ggtgacagga acgcagatga
     4981 tgctgaatgt ttctgcgtcg aagcagtagc accaatctgc cttgcacgac aaaagcggtc
     5041 cgcagtgggc cgcttttgct ttattcaggg gttataaatt cccgcgagca gcctgttcgc
     5101 gctcgatggc ttcaaacagc gccttgaagt tgcctttccc aaagctgcgg ctgcctttgc
     5161 gctggatgat ctcatagaag agtgtcgggc ggtcttctac cggacgcgta aagatctgca
     5221 acatatatcc ttcatcatcg cggtctgcaa ggattccaag cttttccagc tcctgaatcg
     5281 gctcgtcaat tttcccgact cgcttttcca gctccgtgta atacgtatgc ggtaccttga
     5341 ggaattcaac tccctgtgcc tggagctgcg aaaccgtgtg cagaatatcg ttcgttgcca
     5401 tggcaatgtg ctgcacgccc gggccgtgat aaaactccag atactcttct acctgcgatt
     5461 tcctgcggcc ttcggctggt tcgttgatgg ggaacttcac gcgtccattg ccattcgcca
     5521 tgactttcga cataagcgca gagtattccg tcgaaatgtc cttatcgtca aaatgctgat
     5581 aaagcgagaa ccccatcaca tccgcataga aatccaccca gcggttcatc tcattccagc
     5641 cgacgtttcc caccatatgg tcaacgtgca gcagcccgac cggacgcgca agtttgtctt
     5701 cttccactgg gcgatatccc ggcataaaga cgcctttgta ctcactgcgc tcaacaaagg
     5761 tatggacagt atcgccatag gtggcaattg tggaaagcct tacgacgcca tgctcatcgc
     5821 gcaactcaaa cggttcacga atgctccgcg cgccgcgctt tgtcgtctcc ttccatgcat
     5881 atttcgcatc atctacccac agcgcgatgt cgtgtacacc atccccatgc cgatgcacat
     5941 gctcagcgat ctcaccgtca ggccgaagcg gagtcgtaag cacaaaacga atcttgccct
     6001 gttgcaaaac ataagaagcg cggtcacgaa caccggtttc aggcccggca taagcaacga
     6061 gcctcattcc gaaagcgagc cggtaataat aggcagcctg ccgcgcattg ccaacataga
     6121 actcaacata atcggtcccg ttgagcggga gaaaatcttt ttgcgtttgc acttccggat
     6181 gcgcaagagt ggacatcgaa gttactccat cgaatgaatt agaagcgcca gccaacggtt
     6241 cctgctccta tggcgaccta ttaacatata ccgttgagag cacagttgga aagggcagga
     6301 gtgtgcatcc atgtcattca aaactgcggg ccatgatgtc ctgctgatca ttgacgttca
     6361 gaacgatttc tgccccggcg gcgctctcgc cgtcccccac ggagacgaga ttgtcccagt
     6421 catcaaccag cttgcgcaac agttcgccca cgtcattctt acgcaggact ggcacccaaa
     6481 aggccatctg tcttttgctt cacgccatcc tggaaagtcc ccttttgatg tggtcgaagt
     6541 ttcctatgga ccgcagacgc tctggcccga ccactgcata caggcaagtc gcggagcaga
     6601 gtttcacccc gacctgaagg tcccgcatgc ggaactggtc ctgcgcaaag gcttccggcc
     6661 cgaaattgat tcgtactcgg cctttcagga aaacgacagg aaaacaaaaa ccggtcttga
     6721 aggttatctg cgcgagcgcg gatttcaacg ggtatttctc gcggggcttg cctatgacta
     6781 ttgcgtgcgg tattc
//
LOCUS       DASRBV010000416         5354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_50132,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000416 DASRBV010000000
VERSION     DASRBV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50132"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFA02_14870"
     CDS             complement(<1..438)
                     /locus_tag="VFA02_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU11185.1"
                     /translation="MLHEILPVGLLQCNCSVVADDEIREAIVIDPGDEIERVQKILAR
                     HDLTVKYIIATHAHIDHVGGLKKLRELTGAPVLMHEADLPLYQNLATQAAWLGVEAPG
                     MVEVDQFLKEGDRLRWGSLSLEVLHTPGHSPGSLSLHLPGEHAR"
     gene            633..863
                     /locus_tag="VFA02_14875"
     CDS             633..863
                     /locus_tag="VFA02_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11186.1"
                     /translation="MALGATGLGISFRGKTALLRFALRGLDVPDDGAYFSYMTPAVLA
                     LSFAGLQMAQIFDGQVRGASPESARHARHDKS"
     gene            942..1232
                     /locus_tag="VFA02_14880"
     CDS             942..1232
                     /locus_tag="VFA02_14880"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZU11187.1"
                     /translation="MAALSKVEIQQKLKDMRGWSHMGKAIQKRYTLKSFMPAISLVNK
                     IAELAEKAGHHPDITINYNVVGISLSTHSEGGVTQKDFDLARQIDQLAEAQS"
     gene            1311..1730
                     /locus_tag="VFA02_14885"
     CDS             1311..1730
                     /locus_tag="VFA02_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11188.1"
                     /translation="MATFAQWVHLSAAVIGVGGMAFMLFVLFPSADVLDEERRNGLLK
                     AVFSRFRWVSWTVILLLLASGLYNVKLVWLVPWGPYWKFLTAKIVLALLIFIIVLCLT
                     IPLSIFNGFRRRRALWLSIAFGLAMAVILISAYLRRG"
     gene            complement(1832..2725)
                     /locus_tag="VFA02_14890"
     CDS             complement(1832..2725)
                     /locus_tag="VFA02_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU11189.1"
                     /translation="MGDYSSHFDQGFYVTPRGDVFALVQACPYKDGCKPPHFPVNLIV
                     KYNHDASVDSIIKLTPPSDLPFLAFKFAVFLDGNVLVTGLEGAAEEDEAGRSHYRPSK
                     PFTGIFDRDGAYVGPLSLPNDITPPPNAAAETAPKKGSPAGATGATGPEQSVATAKPG
                     TFWAQGVIGSVLMGGLDGNVYLLRASYPPLLYSISPDGRVLDERTMTLSQPGMWPTLG
                     SFTQDGRLFVEFSQAPTAPRNERLHPIFAVIDPQTGSILETYDVPSTAGIPACMDPQN
                     ELLFLRSDKTGQLEVAKFVAH"
     gene            complement(3765..4793)
                     /locus_tag="VFA02_14895"
     CDS             complement(3765..4793)
                     /locus_tag="VFA02_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZU11190.1"
                     /translation="MKAVRFHEHGGLDVLKYEDAPEPQIQANEVLVRVKACALNHLDL
                     WLRTGVRAWQLPMPHIVGSDISGEVAQAGNLVPHVKPGDRVLLAPGISCGLCEACWKG
                     LDSACRSYTLFGVMVDGGYAEYVKSPAANVIPIPGDLTFDEAAAVPLVFLTAWHMLFT
                     RARLQPGEDVLVIGAGSGVGSAALQIAKLFGCRVIATAGTDAKLAKARELGADDGINH
                     SKQSISEEVSRLTAKRGVDVIVEHVGQAVWDECFNSLATYGRLVTCGVTTGGEVKLNV
                     QALFGRQRAVLGSFMGGKGELTDVLKLVGQRRLKAVIDSVFPLSEAREAQRKMESRDF
                     FGKILLRP"
     gene            5044..>5354
                     /locus_tag="VFA02_14900"
     CDS             5044..>5354
                     /locus_tag="VFA02_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZU11191.1"
                     /translation="MSDVFSEIVKLRKEGRRGALATIIQVQGSIPSYECSKILIRDDG
                     SIVGTVGGGCVEADVWAAAQDVMREEKPRRLHFNLNANPEYDTGLVCGGSLDIFIEPI
                     LA"
BASE COUNT         1165 a         1530 c         1496 g         1163 t
ORIGIN      
        1 acgagcatgc tctccgggga ggtgaagcga aaggctgccc ggcgagtgcc ccggcgtgtg
       61 caggacctcc agagaaagcg atccccatcg cagccggtcg ccttccttta aaaattgatc
      121 cacttccacc atgccgggcg cttccacgcc gagccatgcg gcctgcgtgg caaggttctg
      181 atagagcggc aggtcggctt cgtgcatcaa taccggcgcg cctgtcagtt cgcggagctt
      241 cttcagccca ccgacgtggt caatgtgcgc gtgcgtggcg atgatgtact ttacggtgag
      301 atcgtgccgc gcgagaatct tctgcacacg ctcgatctca tcgccgggat cgatgacaat
      361 tgcctccctg atctcgtcat cggccaccac cgagcaatta cactgaagca ggccaacggg
      421 aagaatttcg tgaagcatag caactgaaat tctagtccag tgcgtcaaag gcgtgcacac
      481 ttatttcaag ggcatggtgc tctaccgttg caatgcgccg ccggggctta tctcgccatg
      541 cgtccacgtg aaggtggacg cgatgtgaac cgatattgct gacgcactga actggtgtag
      601 cgtagcaaaa gaagctagac aaagtctatg gattggcact cggagcgacg ggcctcggca
      661 tttcttttcg aggaaaaacc gcgctgcttc gttttgctct gcgtggtctt gatgtgcctg
      721 atgacggtgc ttatttcagt tacatgacac cggcggtctt ggcgctctct tttgcgggat
      781 tgcaaatggc acagattttc gatggtcaag ttcgcggtgc aagtccggag tcggccaggc
      841 atgctcgcca cgataagtca tgaaagcaaa cgaccgcccg ttgacttgtc tccgagggct
      901 ttttatactc aagccggaat gagcaaggca aaggaggggt tgtggcagca ctcagcaagg
      961 ttgaaattca gcagaagctg aaagacatgc gcggctggtc gcacatggga aaagccatcc
     1021 agaagcggta cacgctgaag tccttcatgc ccgccatctc actggtgaac aagatcgccg
     1081 aactcgctga aaaggccgga caccatcccg acatcacaat caactacaac gtggtgggca
     1141 tctctctttc cacgcacagc gaaggcggcg tcacccagaa ggatttcgac ctcgcgcgac
     1201 agatcgacca gttggcggaa gcccaatcgt aggatggcct gcttttcgcc gcattcggaa
     1261 caaaagggac ccgcgggcag cagatccatc cacgacataa ggaaaacgta atggcaacat
     1321 ttgcgcagtg ggtccacctg agcgccgcgg tgatcggcgt gggaggaatg gccttcatgc
     1381 ttttcgttct tttcccctcg gccgacgttc tcgacgaaga gcggcgcaac ggtctgctga
     1441 aggccgtatt cagccggttc cgctgggttt cgtggacagt catcctgctg ttgctggctt
     1501 cggggcttta caacgtcaaa ctggtctggc ttgtcccctg ggggccttac tggaaattcc
     1561 tgaccgccaa aatcgtgctt gcgttgctca tcttcatcat cgttttgtgc ctcaccatcc
     1621 ctctgagcat cttcaatggg ttccgtcgcc gccgcgccct gtggctctcg atcgccttcg
     1681 gactcgccat ggcagtgatc ctgatctcgg cctacctgcg ccgagggtga tccgtcagga
     1741 aatacgggaa ggtagcctgc tgcctccgac cgctttgccc tgtacgttac ggacttgcgc
     1801 cttccagacg gcaagaactg catttactct gttaatgcgc cacaaacttt gcaacttcga
     1861 gttgccccgt cttgtctgat ctcaggaaca gcaattcgtt ctgtggatcc atgcaagctg
     1921 gaatgccagc cgtagatggc acgtcgtacg tctcgaggat cgacccagtc tgcgggtcaa
     1981 tcaccgcaaa aatcggatgg agtcgctcat tccggggcgc cgttggtgcc tgagagaatt
     2041 ctacgaacag tcttccatcc tgcgtaaagc tccccagtgt gggccacatg ccgggttggg
     2101 aaagtgtcat ggtgcgctcg tcaagaactc tgccgtcggg tgagatcgag tagagcaggg
     2161 gcgggtagct cgcacgcagc agatagacgt ttccatccaa cccacccatc aacacgcttc
     2221 cgataacgcc ctgtgcccaa aaggtccccg gcttggccgt ggcgaccgat tgctccgggc
     2281 cagttgcgcc ggtagctccg gccgggctcc ctttcttagg tgccgtctca gccgccgcat
     2341 tgggaggtgg cgtaatatcg ttcggtaaac tgagcggccc gacgtaggct ccgtcacggt
     2401 caaaaatacc cgtaaacggc tttgaaggcc ggtaatgaga gcgccctgct tcatcttcct
     2461 ctgccgctcc ctccagaccc gtcacgagca cattcccatc aagaaacact gcaaacttaa
     2521 acgccaggaa cggcagatca gaaggggggg tgagtttgat gatggaatcc acagaagcat
     2581 cgtgattgta cttgacaatc agattaaccg gaaagtgcgg aggcttgcag ccgtctttgt
     2641 aagggcaagc ttggacaagc gcgaaaacat ctccgcgggg cgtcacataa aagccctggt
     2701 caaagtgcga ggaatagtct cccaatggag ggtattgaaa ccgggtcgca ctttttgact
     2761 ccatggaaag tttggtgagc ggaggacggt tgcccgatcg agcgttttgc tcgataaact
     2821 ctaaagacgg ggtcgattcg aaatatacat tcccatcggc atcgcactgc gtccctgcga
     2881 aaacagcgac tggccccgga acgccaaagt cggctgtgcc ggcagactcg agctcccgta
     2941 ctggcgggga ctcggcgcgt tgtttagttt gtcgttcttt ttggacctca ccttgcgagg
     3001 tacgcaggcg tcccgctgtc agggagaatc ccgtcaggat catcacgaaa acaagcccgg
     3061 aatgccttcg acaagacatg acgttcttcc tcctttctcc aacggctttg ccgaggcgct
     3121 tcaggcaata agacctgaaa cgccccagtc tctaagcgca aatcatgtta gcggtccttt
     3181 gctatctcca taaacgagta attcccatcg cacccgcgca cgtaagcgta aagcggaggg
     3241 cggtacgcgg attaggctga gggaggctgc ttcgtgggtg acgctaaact gcagtttgtg
     3301 ccggtgaaaa gccagtctgt gtaagtgccg cactcacaaa ccacgcactg gctttggatg
     3361 gtttcgccgg tgtagtagct gtagccgcaa acgtggaaca aacaggagcc tgacgggcaa
     3421 ttaacttgaa catcgtccag ggaaagacag cagccgtact ggccatatgt gcacgaggcc
     3481 acgaactgag agcttaatat tagcgcggcc ccaatggtta aaattacccc aaaccaacac
     3541 ttatttttgg gtctttgggg gtcatgaatc acctccacgc gcaaaggttg gaatgttgaa
     3601 gagattctat tcagcggatg ttaccatgtc aatgccttgt tacaagtttg tataacaagc
     3661 tgtagagtcg atattgacaa tctgtttaac tcttccaccg ctttaaggga gacccgctgc
     3721 aagggccggc cccaatctga tggatagtgc catctccata ggtcttacgg gcgcagaagg
     3781 atcttgccga agaaatcacg gctctccatc ttcctctgtg cttcgcgtgc ctccgacagt
     3841 ggaaacacgg agtcaatgac cgccttcagc ctgcgctgcc cgaccagttt gaggacgtcc
     3901 gttaattctc ctttgccgcc catgaatgaa ccgaggaccg cgcgttgccg tccgaacagc
     3961 gcctgaacat tgagcttgac ctcgccgccg gtcgtcacgc cgcaggtcac cagtcgcccg
     4021 taggtcgcca gcgaattgaa acattcgtcc caaacggcct ggccgacgtg ctcgacaatc
     4081 acgtccacgc cacgcttcgc ggtcagacgc gagacttctt cggagatgga ttgcttcgag
     4141 tggttgatgc catcatccgc gcccagttcg cgcgctttcg caagttttgc atcggtcccg
     4201 gcggttgcaa tcacgcggca cccaaaaagt ttcgcgatct gtagggctgc gctgccgacg
     4261 cccgagcctg ctccgatcac cagcacatcc tcaccgggtt gcagcctcgc acgcgtgaac
     4321 agcatgtgcc acgccgtcag gaagacgagc ggtacggcag ccgcctcatc gaaggtcagg
     4381 tcgccgggaa ttggaatcac attggctgcg ggggacttta cgtattccgc gtagccgccg
     4441 tccaccatca ccccgaaaag cgtgtagctg cggcaggcgc tgtcgagccc tttccagcag
     4501 gcctcgcaca atccgcaact gattccggga gccagcagga ccctgtcccc cggcttgacg
     4561 tgaggtacca gatttcccgc ttgtgcaact tcgcctgaaa tgtcgctgcc cacaatgtgc
     4621 ggcatcggaa gctgccaggc gcgcacgccc gtgcgcagcc aaagatcaag atggttcagc
     4681 gcgcacgcct tgaccctcac cagcacttcg tttgcctgga tttgcggctc gggcgcgtct
     4741 tcgtatttca aaacatccaa cccgccgtgc tcatggaacc ggaccgcttt cattcaaaac
     4801 ctcctgattc agatgctggt atgttaacaa atcttgacag gtccaccgac cggggcattg
     4861 tcacttgagc gacggaaaga agcacccgcg caatattccg aagggaacaa ggccggcgca
     4921 gaaaatctta cgcgtcctcc cttttggcgt ctttgcacac cggcgtgatg ctttttgtca
     4981 tgaaatgaaa gcagagtgct gattgacaac aataccgtgc cagattaacc tgaaaataat
     5041 tttatgagcg acgttttcag cgaaattgtg aagctgcgga aagagggtcg caggggagcg
     5101 ctcgccacga ttattcaggt gcaaggttca atccccagtt acgagtgctc caagattctg
     5161 attcgcgatg acggttcgat tgtcggaacg gtgggtggcg gatgcgtcga ggccgatgtc
     5221 tgggcggcag cgcaggatgt gatgcgggag gagaagcctc gccgcctgca cttcaacctg
     5281 aacgccaatc cggagtatga cacgggactg gtttgcggcg gctcgctcga tatttttatc
     5341 gagccgatcc tggc
//
LOCUS       DASRBV010000417         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudacidobacterium sp. isolate SMAG_U9936 k141_50544,
            whole genome shotgun sequence.
ACCESSION   DASRBV010000417 DASRBV010000000
VERSION     DASRBV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963465
            Sequence Read Archive: SRR5579884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudacidobacterium sp. (soil metagenome)
  ORGANISM  Pseudacidobacterium sp.
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae; Pseudacidobacterium.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 18:57:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,946
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Pseudacidobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50544"
                     /isolate="SMAG_U9936"
                     /isolation_source="soil"
                     /db_xref="taxon:2952004"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1248)
                     /gene="rapZ"
                     /locus_tag="VFA02_14905"
     CDS             complement(316..1248)
                     /gene="rapZ"
                     /locus_tag="VFA02_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZU11192.1"
                     /translation="MPSKSARKTAGGPAKKKKDSPALVPVESRVLVIITGLSGSGKLS
                     ALKAFEDMGYYAVDNMPIELVPRFAELIGESATTTRAALVVDAREGRGLERFPQMLTH
                     IRHILPATVVYLEASEPVLLRRFSETRRPHPLGRQEMVASAIRSERKLLDPVRNVADV
                     IIDTSNFNVHELRAYIQDKFAGTGDGKDLLISSISFGYKNGIPLEADLVFDVRFLPNP
                     HFIPEFRPLTGKHPKLVAYLRKFPQTREFLDRVTKLLLYLLPHYIHEGKSYLTIAFGC
                     TGGQHRSVMIAEEIGKRLGKEGYRVKTAHRDMPR"
     gene            complement(1415..2071)
                     /gene="raiA"
                     /locus_tag="VFA02_14910"
     CDS             complement(1415..2071)
                     /gene="raiA"
                     /locus_tag="VFA02_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZU11193.1"
                     /translation="MQVEYTGRQVTVTPALRAIADEFIARIAKILGKTTGAHVVLTAE
                     KYRQFAEVTITTRLQNIVAMSESNNMETALREALDKAETQAIRCKKKIQAIKRQPKEE
                     KLAAEPQLARPRRAARIPPLAEEAKEPPAPKKPNGNGHTRQMVPVTVHSFPARVPVHE
                     PHIVRSADSVAIRPMTLEEAVKEAEFRDRDVFVFRDHEGQVKVLHRKRDGKMELIEAP
                     "
     gene            complement(2145..3812)
                     /gene="rpoN"
                     /locus_tag="VFA02_14915"
     CDS             complement(2145..3812)
                     /gene="rpoN"
                     /locus_tag="VFA02_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZU11194.1"
                     /translation="MALLQPKLSLKVSQRQILTPGLMQMVSVLALNKLELKEMIQSEI
                     AENPILEEMEENVPLLDDVASREEQLAQPAETEFGDAALKKADPFDEIDFGSYFQEYL
                     DPGFRSPDSFEVTEKPSIENFLSKPSTLTDHLFWQLGSLNLSPAVRDAAEYVIGNLNE
                     DGYLTATDEELLEGYLREQLEPEPENSHSPQEHSVLHFAEVPEAMRVRARGHLAAALD
                     VVRTLDPLGVGARDLRECLLLQLDAQRHEFDLIYKRQKNNGHAPALPEIVELLEHDAH
                     ALNGNGMAGRIAVFEAAREIADKHLLLLQRRDMREIAKAAGCSPEIAQQAVDLIRTLD
                     PRPGQRYNRSDARLIEPDVAFVKRGDEYVVMMNDEDMPTLRLNQGYRRMITQDGTEKD
                     VKDYVKERYRSALQLMRNIEQRKNTILRTCEAIVRRQSEFLEKGVDALKPMMIKEVAE
                     EIGVHPSTVSRAVSNKYVHTPQGVYELRFFFSEGVNGPEGAGTPLMLLKRKVKKLIEE
                     EDPRKPLTDDQIANILQSQGISVTRRTVAKYREDMRIPSTHQRRVRS"
     gene            complement(3934..4662)
                     /gene="lptB"
                     /locus_tag="VFA02_14920"
     CDS             complement(3934..4662)
                     /gene="lptB"
                     /locus_tag="VFA02_14920"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZU11195.1"
                     /translation="MQKLSTDEIGKSYKGRQVVRGVSLEISQGEVVGLLGPNGAGKTT
                     SFYMIVGLVPPDSGRILADGEDITRVPMYLRARQYGISYLPQEPSVFRKLTVEENILA
                     VLEVQPLTPEMRRARTEKLIDQLNLGHIRKTRGYALSGGERRRVEIARCLCIQPSFIL
                     LDEPFSGIDPIAVLDLQEIIYDLKASGIGVLITDHNVRETLSVTDRAYIINEGRIFRH
                     GTPEQLGSDPEVRRVYLGEGFSLN"
     gene            complement(4671..6986)
                     /gene="lptC"
                     /locus_tag="VFA02_14925"
     CDS             complement(4671..6986)
                     /gene="lptC"
                     /locus_tag="VFA02_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZU11196.1"
                     /translation="MTITIKRIRAVIVALACLLLVVVGGFLVYARYRIRHIGKDLPGK
                     LGVNIQRTANGFTYSQSQKGHTLFTIHASRLVQFSSDGHATLHDVAITLYGPPGTNRE
                     DRVSGSDFDYDQKNGIATAMGEVQMDLEAPQNSSDAGNAEKNTIHVKASGVVFNQKTG
                     TATTDQFVEFHLPGATGQAIGATYDSKQGILVLDRQVAITSSTNGGPAILHAAHAQLL
                     RDSRQAFLLQAESEFRNARSTADQAIVYFRKDGSAEKIEAKGNVHTVMDNGAQSNCQA
                     TTIYLDEKSRPMQALLGGGINFVDNDPLRTMHGNAVEGTLTFGANSELRHAQFRNAVS
                     FVDQQKLTNDPNGSATRQIAASRLDIDFAPGPDKSSVAQKVLATGNAVATLRTIPSKG
                     APQSTTIYGDQLLATLEGGREIRQLDGSGHTKIMDTAKDGAANVTTSDMLRITFANQK
                     TPKNIQESQIDTAIQEGNVTLTQTPAKSAKAASPFHAWASRAEYHASDQVVHLTGSPR
                     VNNGEVDLTAQTIAYQRDSGDATATGDVRATYLQAKDQAQHGAMFGGQGATHIIASQA
                     ELHHSSSDSIFRGDARMWQQSGNTVTAPVIELSRTQQTLKAFGGNVDTSIASIMGPKK
                     QQSVVRIRSRMLVYSDKDRRGDFSGGVLAVAPNGTIRSDQAQFFLGPAKKPPSATAAV
                     TPSSQIEKIIASGHVLLTQPGRKGEGEQLVYTAADGRYVLTGTPAAPPYVMDAVKGTT
                     TGAALIFNDQDDSVEVSGGQSAAVTKTRAPK"
BASE COUNT         1410 a         1876 c         2009 g         1731 t
ORIGIN      
        1 aggactacgc gcgaatcttt tcatcactga tttcatccaa aagaggaaac cgggagatga
       61 aacgatggcg gctttttgtg catgttgcgg agcagaaatt acacctaagg cagaggcttg
      121 tgcagtatgc ggcactccgc aacacgggag cctgcctgtg aaattttcag aaacattggg
      181 tggtcgcaag accctctcaa cgggacctgg agataccgcc tcaggtgagc gcgcaagtgt
      241 ttccgggggc cctgactgaa gcggtctgac ttcaaaaagc aggttctgcg cttctccgag
      301 gatgacaatt gttggtcagc gtggcatgtc gcggtgcgct gttttcacgc gatagccttc
      361 tttccccaga cgcttcccga tttcttcggc aatcatcaca gagcggtgct ggcctccggt
      421 gcatccaaag gcaatggtca gataactctt tccttcatgg atgtagtgcg gaaggagata
      481 gagtagcagt tttgttacgc gatcgagaaa ctcgcgcgtt tgcggaaact tgcgaagata
      541 tgcaaccagt tttggatgtt tgccggtgag ggggcggaac tccggtataa agtgcggatt
      601 cggcaggaag cgcacgtcaa agaccagatc agcttcgagc ggaattccgt tcttatatcc
      661 aaaactgatg gaggagataa gaaggtcttt gccatcgcct gttcctgcaa atttgtcctg
      721 aatataagca cgcaattcgt ggacattaaa attcgaagta tcaatgatta catctgcaac
      781 gttgcgtacc ggatcgagca gcttgcgctc agagcggatt gcagaggcca ccatctcctg
      841 ccggccaagc ggatgcggac gccgcgtttc agaaaagcgg cggagaagca ctggttcaga
      901 cgcttcaaga taaaccaccg ttgcgggcag gatgtgccgg atgtgcgtga gcatctgcgg
      961 aaagcgttca aggcccctgc cttcgcgcgc gtccactacc agagcggccc tggtggttgt
     1021 ggccgattct ccaatcagtt cggcaaaacg tgggacaagc tcgatcggca tgttgtccac
     1081 cgcgtaatag cccatgtctt caaacgcttt gagcgcagaa agctttcctg agccggaaag
     1141 gccagtgata atgacgagca cgcgggactc aaccggcacc agagccggag agtctttctt
     1201 cttttttgcg ggaccacctg ctgttttgcg cgcagatttg gacggcatgt gcccatcgta
     1261 ccattggttt caccggttag tagcgggttg ttagctccca gttcccagtt gttagtcccc
     1321 agtttccaga tgtcagtcct cagttggtgg catattggag actatcaact gaaaactgtg
     1381 gaccaataac tggcggctga ggactggtga ctactcacgg tgcttcgatc agttccattt
     1441 tgccgtcacg tttgcgatgc agcaccttta cctggccttc atggtcgcgg aagacgaaca
     1501 cgtcacggtc gcggaactct gcttctttga cggcttcctc cagggtcatg ggccggatgg
     1561 ctacggaatc cgctgatcgc acgatgtgcg gttcgtgcac gggaacacgc gcagggaagg
     1621 aatgtacagt aacgggcacc atctgtctgg tatggccgtt tccgtttggt tttttcgggg
     1681 cagggggttc cttggcctct tctgccaaag gggggatacg cgcagcacga cgcggacgcg
     1741 caagctgcgg ttcagcagcc agtttttcct ctttcggctg gcgtttgatt gcctggattt
     1801 tctttttgca gcggatggcc tgtgtttcgg ccttgtccag tgcctcgcgc agcgccgtct
     1861 ccatgttgtt cgattccgac atggcgacga tgttttgcag acgcgttgtg atggtgactt
     1921 cagcaaattg acggtatttt tctgccgtca gcacgacatg ggcgccagtt gttttcccaa
     1981 ggatttttgc gatgcgcgcg atgaattcat cagcaattgc gcgaagagcg ggggtgacgg
     2041 tcacctgcct gccagtgtac tcaacctgca tggcgaaagc tcccttccca aagattttaa
     2101 cgccgaagat gtgttgtcgc tccggcgggt tcagtgattc tacatcaact ccgtacacgc
     2161 cgctggtgcg tgcttggaat tctcatgtcc tcacggtatt tggccacagt gcggcgggtc
     2221 acactgattc cctgtgactg gagaatattc gcaatttgat catctgtgag tggtttgcgg
     2281 ggatcttcct cctcgatcag ttttttcact ttgcgcttca gcagcatcag cggagtgccg
     2341 gcgccttccg gcccatttac tccttctgag aagaagaagc gcagctcata cactccctgg
     2401 ggcgtatgca cgtatttgtt agatacggct cggctcacag tggatggatg cacgccgatt
     2461 tcttctgcaa cttccttaat catcatcggc ttcagagcat ctacgccttt ttcaaggaac
     2521 tcactttgcc tgcgaacaat ggcctcacat gtgcgcagaa tggtgttttt acgctgctca
     2581 atgttgcgca tgagctggag cgcagagcgg taacgctctt tcacgtagtc ctttacgtct
     2641 ttttccgtgc cgtcctgagt aatcatgcgc cggtagccct ggttcaggcg cagcgtgggc
     2701 atgtcctcat cattcatcat gaccacatat tcatcgccgc gctttacaaa ggcgacgtca
     2761 ggctcaatga ggcgcgcatc tgagcggtta taacgctgtc cgggacgtgg gtcgagagtg
     2821 cgaatcaggt ccactgcctg ctgtgcgatc tctggtgagc agccagcggc ctttgcaatt
     2881 tcacgcatat cgcgccgctg aaggagcaga aggtgtttgt ctgcaatttc gcgcgctgct
     2941 tcaaaaacag caatgcgccc ggccattccg ttgccgttca gagcatgagc atcatgttca
     3001 agaagttcga caatttcggg gagggcaggg gcatggccgt tatttttctg ccgtttgtag
     3061 atcaggtcga attcatgacg ctgggcatca agctgcaaca gcaggcactc gcgtaggtcg
     3121 cgcgcaccta ctcccagagg atcgagggtc cggacgacat ccaatgccgc ggccaggtgg
     3181 cccctggccc ggacgcgcat ggcttcgggc acttcagcaa aatggaggac cgaatgctct
     3241 tgcggagagt ggctgttttc cggttccggc tccaactgtt cgcgcaggta accttcaagc
     3301 agttcttcat ctgtagctgt taaatagccg tcttcattga ggttgccgat aacgtactct
     3361 gcggcatccc gaaccgcggg cgacagattc agggaaccaa gctgccagaa gagatggtcc
     3421 gtgagcgtgc tcggcttgga gagaaagttt tcaattgagg gtttttctgt aacttcgaag
     3481 ctgtccgggg aacggaagcc ggggtcgaga tactcctgaa agtaggagcc gaaatcaatt
     3541 tcatcgaagg ggtcggcttt tttgagcgcc gcatcgccga attcggtttc ggcgggctgg
     3601 gccaattgct cctcgcggct ggcaacgtca tccagcagtg gaacgttttc ttccatctct
     3661 tcaaggatgg ggttttcagc aatttcagac tgaatcatct ccttcaactc aagcttgttc
     3721 agggcgagca cgctgaccat ctgcatcagg ccgggcgtga gtatctgccg ctgagatacc
     3781 tttaagctga gtttgggttg aagaagcgcc attctttacc tatatgtctg ctctcgtggg
     3841 ggagataacc acagtctaca cgcttcaatg gggccactgg taggccggaa ctcagtacaa
     3901 gcggcagttt cagtgatacg gaagtcatct ggtttaattc agcgaaaacc cttcgcccag
     3961 gtagacgcgc cgcacctccg ggtcactgcc caactgttcc ggcgtcccgt ggcggaagat
     4021 gcgtccttca ttgatgatgt acgcacggtc cgtcaccgaa agcgtttcac gcacgttatg
     4081 atctgtaatt aacacgccaa tgccgcttgc tttcaggtca tagataattt cctgaaggtc
     4141 gagtaccgca atgggatcaa ttcccgaaaa gggctcgtcc agcaaaataa aagatggctg
     4201 gatacacaga caacgggcaa tctctacacg gcgccgttca ccaccggaaa gcgcatagcc
     4261 gcgcgtcttg cgaatatggc cgagatttaa ctggtctatc agcttttccg tgcgcgcgcg
     4321 ccgcatctcg ggtgtaagcg gctgcacttc cagcactgcc agaatgtttt cttcgacagt
     4381 gagcttgcga aagacagacg gttcctgtgg gagatagctg atgccatact gccgagcgcg
     4441 cagatacatg ggaacgcgcg taatgtcttc gccatcagca aggatgcgtc cggaatctgg
     4501 cggaaccagg cccacaatca tgtagaagga ggtggttttt cctgcgccgt tcggaccgag
     4561 aagcccgaca acctctccct gcgaaatttc aaggctcacg ccgcggacga cctgacggcc
     4621 tttgtaagac ttcccgattt catcggttga aagtttttgc atttacaaat ttacttagga
     4681 gcgcgcgtct ttgtgacggc cgcggactgc cccccactca cctcgacgct atcatcctgg
     4741 tcattgaaaa tcaatgcggc gcctgttgtt gtgcctttga cggcatccat gacataaggc
     4801 ggggccgcag gggtccctgt gagcacgtat ctgccatcgg ccgcggtgta gacgagttgt
     4861 tctccttctc cttttctgcc cggctgcgtg agtaagacgt ggccggaggc aatgattttt
     4921 tcaatctgcg aggagggtgt gacggcagcc gtggcggacg gcggcttttt cgcaggcccg
     4981 aggaagaact gtgcctggtc agagcggatg gtgccattcg gagccacggc cagtacgcct
     5041 ccgctgaagt cgccacgacg gtctttgtca gaatagacga gcatccggct cctgatgcgc
     5101 acgacgctct gctgtttttt cgggcccatg atggaagcga tggacgtatc tacatttcct
     5161 ccgaaggcct tcagtgtctg ttgtgtgcgt gaaagctcga tgactggagc agtgactgtg
     5221 ttgccggact gctgccacat gcgggcgtct ccgcgaaaga tgctgtcgct gctcgagtga
     5281 tgcaactctg cctgcgatgc aatgatgtgg gtcgcgccct gcccgccaaa cattgccccg
     5341 tgctgcgctt gatctttggc ctgcagatag gttgcccgca catcgccagt tgctgttgcg
     5401 tcaccggagt cacgctgata ggcgattgtc tgtgctgtaa ggtccacctc accgttattg
     5461 acgcgaggac ttccggtcag atgcaccacc tgatccgaag catgatattc cgcccggctg
     5521 gcccatgcgt ggaagggtga agctgctttc gcgctttttg caggagtctg cgtcagggtc
     5581 acattgcctt cctgaatggc ggtgtcaatc tgcgactcct gtatgttttt cggcgtcttc
     5641 tgattggcaa acgtgatgcg cagcatgtcg cttgtggtca cgttggcagc accgtctttg
     5701 gctgtatcca tgatctttgt gtgcccagag ccatcaagct gacggatttc ccgtccgcct
     5761 tccagagtgg ccaatagctg gtctccatag attgtcgtgc tctgtggagc acctttcgat
     5821 ggaatggtgc gcagggtggc cacagcgttt ccggtagcca ggaccttctg tgcaacggac
     5881 gatttgtctg ggccgggcgc gaaatcaata tccagccggg aagctgcaat ctgccgcgtg
     5941 gctgaaccat tcggatcatt cgtcagtttc tgctggtcta caaaactgac ggcattgcga
     6001 aattgtgcgt gcctgagttc ggaatttgcg ccgaatgtca aggtgccttc cacggcattt
     6061 ccatgcatcg tccgtaatgg gtcgttatcc acgaaattaa taccgccgcc aagcagtgcc
     6121 tgcatgggcc ggcttttttc gtcgaggtaa atcgtcgtgg cctggcagtt ggactgcgct
     6181 ccattgtcca tgacggtgtg aacattgcct ttggcttcaa tcttctctgc cgagccatct
     6241 ttgcggaagt agacaattgc ctggtcggca gtgctgcgtg cattgcggaa ctcgctttct
     6301 gcctgaagca gaaatgcctg tcgtgagtcg cgcaggagtt gcgcgtgggc cgcgtggaga
     6361 atggcagggc cgccattggt gctcgacgtt atcgccacct gcctgtcgag cacgagaatg
     6421 ccctgcttag aatcataggt cgcgccgatg gcctgtcccg tggcgccggg cagatggaac
     6481 tctacaaact gatctgttgt agcggtcccg gtcttctgat tgaagaccac tccgctggcc
     6541 tttacatgga tcgtgttttt ctcagcgttt cctgcatcgc tgctgttctg aggggcttca
     6601 agatccatct gcacctcgcc catggctgtg gcaataccgt tcttctggtc gtaatcaaaa
     6661 tcgctgccag agacgcggtc ttcgcggttg gtcccgggag ggccgtacag cgtaatcgct
     6721 acatcgtgca gagtcgcgtg gccatcgctg ctgaactgta caagcctgga ggcgtgaatg
     6781 gtgaaaagcg tgtgtccctt ttgcgactgc gaataagtga atccgttcgc cgttcgctgg
     6841 atattcacgc ccagctttcc cgggaggtcc ttaccgatgt gccggatgcg gtaacgcgcg
     6901 tagaccagaa atccgccgac cactaccagc agcaggcagg cgagcgccac gatcactgcc
     6961 cgaatccgtt tgatggtgat ggtcatgaaa ttgattatcg cagcaagtca gcaggccagc
     7021 gagtcg
//
